LOCUS       DASRSC010000001        12953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_655052,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000001 DASRSC010000000
VERSION     DASRSC010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12953
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655052"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VFE39_00005"
     CDS             <1..672
                     /locus_tag="VFE39_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET6257010.1"
                     /translation="AVDLPADDVAACVAEVGIGFCFAPVFHPSFRHTAGPRRELGVPT
                     AMNVLGPLTNPAQPPAALVGCADARLAPVLAEVFASRGSSALVVRGDDGLDELTTTTT
                     STVWVAAGGEVRTETVDPAAFGIVPATREDLRGGSAEVNAEAFRALVAGDRGPVRAAV
                     LLNAAGALIAFDGPPANLADAFPAALERVTAAIDSGAAERLLERWAEFSTSRRLRPLG
                     GSGSP"
     gene            complement(669..947)
                     /locus_tag="VFE39_00010"
     CDS             complement(669..947)
                     /locus_tag="VFE39_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HET6257011.1"
                     /translation="MITAIVLVHTAADRIPETAQAIADLDGVTEVYSCAGEVDLIAVV
                     KVAQHDQLADVIAGGLSKVEGVRRTDTHIAFRSYSRADTEATFSVGLD"
     gene            complement(986..2701)
                     /locus_tag="VFE39_00015"
     CDS             complement(986..2701)
                     /locus_tag="VFE39_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HET6257012.1"
                     /translation="MPGPSPQLSFDELGTPLREVTFVVLDLETTGGSARNDAITEIGA
                     VKVRGGERIGELATLVDPGTGVPPGIVALTGITTAMITGAPRLSAVLPTLLEFLSGAV
                     LVAHNAPFDAGFLRAACERHGRAWPRPPVLCTARLARAVLSPDEAPSVRLGALAQLFG
                     TTTKPTHRALADARATVEVLHHLLERVGNLGVQSLEELLGLARESAQHRPTQQQRKKR
                     VLAAGVPAAPGVYLFRGARDEVLYVGTSGDLQRRVRSYFTSGERRRRVRDMVALAERV
                     DTVVCAHALEAAVRELRLIAAHQPRYNRRSRRPHRGWWVTPTQEAFPRLSVITTPRDG
                     ALGPFGSRRAAAAAVDTVLDVVPLRPCTQRIPARGASAAPCALHELGRCSAPCAGLQS
                     PEEYAPAVQALHDLVGGRDDAALRTLAARVDALAGRERFEAAARARDQLAALIVGLER
                     AQRLATLAALPEVVGARPDGRGGWEIAVLRFGRLAAAGVARRGVAPMPVIDALRLGAQ
                     TVLAGPGPLEGAPVEEVQLLHRWLTTGGTRLVHAEPAWAEPARGAARWSRWAERARPI
                     EVDVD"
     gene            2808..4172
                     /locus_tag="VFE39_00020"
     CDS             2808..4172
                     /locus_tag="VFE39_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005448519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET6257013.1"
                     /translation="MSGPDGTAWPPESGGTGLAAAGGAGASEPVNWERLPDAVRARLA
                     DMAAAAVGAMPAADVPVSLRRLARFTPAKRAKLGGQALTAELAASTAFRTAVVAWWEE
                     HRPGELTVAAHDVLTAAAAAVLADDPCAPEVVAAAARRGEAVELRAERDAALSRVDKL
                     TVELDRLRGELTEARAQARLAGEQRDAEYQQLRRRVSEQGAKLRAVVDQRVTAEQAAE
                     ELRRSAESDLAVARAERDRERGRAEVERRRADRAAAEVAVARQAAREARKADEVRLEL
                     LIETLGGAVKGLRRELALGGGGPRPADLIAGAQAARSGAAIDGLAGLDALLAVPSVHL
                     IVDGYNVSKTGYPELPLADQRTRLVGQLAALAARTGVEITVVFDGAGVVTAPHRGSRG
                     VRVLFSDRGVLADDVIRDLAAAEPEGRPVLVATSDRAVVGSVRRFGAYSVPSAVLLGR
                     LARG"
     gene            4994..5884
                     /locus_tag="VFE39_00025"
     CDS             4994..5884
                     /locus_tag="VFE39_00025"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET6257014.1"
                     /translation="MQHDAEALTEEWHAAQDTVTARQAELQTLQAAVAPAKAAVDTAK
                     ASEEQFRQQVDALAMGTFESGNLDQFNALLASGSPQDFLDQMSALETLSADYKDALAQ
                     LQAVVDKTAKAQSDADDAVARAQTAADEAAKAEQDVAARKRDAEMRIDQAEQLLKQLS
                     PQQRRARNGPDVSAPSGPITGTGVGVEALRAAATQLGKPYKWGAEGPNSYDCSGLTSW
                     AFKRAGVTLPRSSSQQARVGSSVSWDNMRPGDLVFYYSPVSHVGIYAGGGKMIDAPQT
                     GDVVKYQTVSSSAFSGARRL"
     gene            6002..7150
                     /locus_tag="VFE39_00030"
     CDS             6002..7150
                     /locus_tag="VFE39_00030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6257015.1"
                     /translation="MPSTLLVTNDFPPRTGGIQSYLQAFADRLPPGDLAVYAPAWPGA
                     AHFDAARPYPVHRHPTSLMVPTPGVARRAAALAREHGASTIWFGASAPLALLGPGLRR
                     KAGIARLVASTHGHEVGWSMLPGARQALRRIGSHTDALTTISQYTRGRVAAAFGPCAA
                     LEVVPSGIDAEVFRPDTAARVALRRRYGLGDAPVVSCVSRLVARKGQDALIRALPRVR
                     SQVPGARLLLVGSGPYADRLRRLAVDAGVAEHVVFTGAVPAAELPAHHAVGDVFALPC
                     RTRGGGLDVEGLGIVLLEAAAAGLPVVAGDSGGAPETVQDGRTGHVVGGRDLDALVEA
                     VAGLLAHPDRARAMGAAGREWMLRDWTWPARVARLSALLTGSPISAPA"
     gene            complement(7296..9134)
                     /locus_tag="VFE39_00035"
     CDS             complement(7296..9134)
                     /locus_tag="VFE39_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET6257016.1"
                     /translation="MREYSVPATFRVADEDNLVGAVYDNAAQDASAPAFRRRSSSSDG
                     TWSEVTAGQFADEVTAVARGLITAGVEPGDRVALLSRTRFEWAVIDYAVLAIGGVTVP
                     IYETSSAEQINWILTDSEAVAVVVETDKHRALVESVSADIPALRHVWQIDGAAAGPET
                     PPAIEQLAALGSDTPADEVHARRRAVRADDLASLIYTSGTTGRPKGCELTHRNLLFEA
                     KGLAQDFPQVLTAGASFLLFLPLAHVFGRALQYCAVATRTVIGHTPDVSTLLPDLQAF
                     SPTFLLAVPRVFEKVYNGARQRAHDDGKGRIFDAAADTAIAWSRARDTGGPGLGLQLR
                     HALFDKLVYGKLRAAVGGSVVAAGSGSAPLGDRLGHFFRGIGLPILEGYGLTETSAAI
                     TINTLTAQRIGSVGRPVSGHSVRIADDGEILLKGPVVFHRYWKNETATAEALEDGWFH
                     SGDLGEVDDAGFLSITGRKKEIIVTAGGKNVAPAVLEDRLRAHPLVSQCMVVGDAQPF
                     IAALVTIDGDALPGWRERNGKPAGDGAADLVDDPDLRAEIGNAVAEANKAVSRAEQIR
                     EFRILPVDFTEAGGEMTPTMKVKRAVVAKTFAGDIASIYSTTKSPT"
     gene            9247..9639
                     /locus_tag="VFE39_00040"
     CDS             9247..9639
                     /locus_tag="VFE39_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide cyclase / dehydrase and lipid
                     transport"
                     /protein_id="HET6257017.1"
                     /translation="MPSVDVVDETFLAVAPEVVAAEFAQPVRWPQLWPDLRLEVMADR
                     GVQGVRWTVQGALVGSMEVWLEPVLDGTVLHYFLRADPAGPDGTPCPASARRGAAEAR
                     RRQRAAKAIAFACKDRLEAGRAPGEPPT"
     gene            9650..10438
                     /locus_tag="VFE39_00045"
     CDS             9650..10438
                     /locus_tag="VFE39_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6257018.1"
                     /translation="MRVHVVSDVHGNVEALARAGDGADALVVLGDLVDYVDYGAPEAG
                     ILGRVLGPDVSRRFAQLRTNGAPGELVDYVRGAWSRVEDAAVVVEQAVREQYDRLFAA
                     MTAPTYAIPGNVDLPRLWPGYARPGLCLPDGQVVDVGGLRFGFVGGAPLPPGQAPRRG
                     GPWTPHLRLAEEFAAAVHGLGPVDVLCSHVPPAVPELAFDVVSRRPEAAAPALLDIIH
                     RDRPRAALFGHVHQPLAARARVGRTECINVGHFRRTGVPYVLRW"
     gene            10502..10942
                     /locus_tag="VFE39_00050"
     CDS             10502..10942
                     /locus_tag="VFE39_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6257019.1"
                     /translation="MADATTSTITIAAAPERVMAVIADFPAYPEWASQVKSVEVLGSD
                     AQGRAERVKFTMDAGPIKDSYTLDYTWASDARSVSWTLVKGQIQKAQTGSYTVEGTGS
                     ETTVTYSLAVDLNIPMIGMLRRKAEKVIIDTALKGLKRRVESAP"
     gene            10979..12205
                     /locus_tag="VFE39_00055"
     CDS             10979..12205
                     /locus_tag="VFE39_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HET6257020.1"
                     /translation="MRVVLFTGKGGVGKTTLAAATATMLARSGRKVLAVSTDPAHSLG
                     DALEADLGGEPVELEAGLCAAHIDTRALLDDSWGRLQVHLRTMLSGAGVDELVADELT
                     VLPGVEELLALGEVRRAAETGLWEVVVVDCGPTAETLRLLALPEALSGYLERLFPAHR
                     RAVRGLLAGLAGAGRDAQPAWDRTIDALDGLAEQLTGLRSMLADHTRTSIRLVLTPER
                     VVVAETRRTLTALALHGLHVDALVANRVLPGPPPSLRGPAARWLRERHAEQQVVLAEL
                     GAAPVPLRTVAYTAAEPTGVAALLAVAQSLYGSTDPAPPAGPATAPLLQVRRTAGSGT
                     SLDSEFELVLRLPGVAAGSLDLARVEDELAVTAGGTRRMVALPSVLRRCTVTGARLSG
                     DDLCVVFTPDPSVWLR"
     gene            12202..12570
                     /locus_tag="VFE39_00060"
     CDS             12202..12570
                     /locus_tag="VFE39_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257021.1"
                     /translation="MTATPGGCGEHAGLSAELRALALSALDRLEPVLERMRTEPTART
                     AETCTACPVCVLIAALRGEHPDLAGRLAEHATGLVAVLRAALEEGVPASESSPAPEPE
                     PAAPARQVQHIPIDRGPAVG"
     gene            12612..>12953
                     /locus_tag="VFE39_00065"
     CDS             12612..>12953
                     /locus_tag="VFE39_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6257022.1"
                     /translation="MLTVGVDVGGTSVRAGVVDAEGNVHDTARTPTPRSEAALEAALA
                     GVINELAARHRIGAVGLALAGFVTPDRRGVRFAPHLSWRDAPVADRIAERVGLPVVVE
                     HDANAAALAERH"
BASE COUNT         1675 a         4668 c         4827 g         1783 t
ORIGIN      
        1 gccgtcgacc tgccggccga cgatgtcgcc gcgtgcgtcg ccgaggtcgg gatcgggttc
       61 tgcttcgcac ccgttttcca cccgagcttc cggcacacag ccggtccgcg ccgggagctg
      121 ggcgtgccca cagcgatgaa cgtgctcggc ccgctgacga acccggcgca gcccccggcg
      181 gccctggtcg gctgcgccga cgcccggctc gctcccgtgc tcgccgaggt gttcgcgagc
      241 cgcgggtcgt cggcactcgt cgtgcgcggc gacgacgggc tcgatgagct gaccactacg
      301 accaccagca cggtctgggt ggcggccggt ggcgaggtgc gcaccgagac ggtcgatccg
      361 gctgcgttcg gcatcgtccc cgccacccgg gaggacctgc ggggaggctc ggccgaggtc
      421 aacgccgagg cattccgtgc actggtcgcg ggagatcgcg gcccggtgcg cgccgcggtg
      481 ctgctcaacg cagcgggcgc cctgatcgcg ttcgacgggc cacccgccaa cctggccgac
      541 gccttccccg ctgcactgga gcgcgtgacg gcggcgatcg actcgggagc ggccgagcgg
      601 ctgctcgagc gctgggccga gttctcgacc tcccggcgcc tgaggccgct cggcggctcc
      661 gggtcgccct agtccaggcc gaccgagaag gtggcctcgg tgtcggcgcg ggagtacgag
      721 cggaaggcga tgtgggtgtc ggtgcgtcgc acgccctcga ccttgctgag ccccccggcg
      781 atcacgtcgg ccaactggtc gtgctgggcc accttgacca cggcgatgag gtcgacctca
      841 ccggcgcagg agtagacctc ggtgactccg tcgaggtcgg cgattgcctg agccgtctcc
      901 gggatccggt cggcggcggt gtgcaccagg acgatcgcgg tgatcacgtc ggctcctcgg
      961 ggtaggacgg ctgacgggcc ggacattagt ccacgtcgac ctcgatcggc cgcgcccgct
     1021 ccgcccagcg gctccagcgc gccgcgcccc gagcgggctc cgcccatgcc ggctcggcgt
     1081 ggaccaggcg ggtgccgccc gtggtgagcc agcggtgcag cagctgtacc tcctcgacgg
     1141 gtgctccctc caacggtcca gggccggcga gcacggtctg cgcgccgaga cgcagtgcgt
     1201 cgatcaccgg catgggagcg acgccgcggc gggcgacccc ggcggcggcg agccggccga
     1261 accggagcac cgcgatctcc cagccgccgc gaccgtcggg tctggcgccg accacctcgg
     1321 gcagcgcggc gagcgtggcg agtcgctgcg cgcgctcgag cccgacgatc agggcggcca
     1381 actggtcgcg ggcgcgcgcg gcagcctcga agcgctcccg cccggcgagc gcgtcgaccc
     1441 gggcggccaa ggtgcgcagc gcggcgtcgt cgcggccgcc gaccaggtcg tgcagtgcct
     1501 gaacggcggg ggcgtactcc tccggtgact gcagccccgc gcacggcgcg gagcaccggc
     1561 cgagctcgtg cagcgcgcag ggcgctgcgg acgccccgcg ggccgggatc cgctgcgtgc
     1621 acggccgcag gggtacgacg tcgagcactg tgtcgaccgc cgctgccgcc gcacgccggg
     1681 acccgaacgg gcccagggcc ccgtcgcggg gcgtggtgat caccgagagc cgcgggaacg
     1741 cctcctgggt gggggtgacc caccacccgc ggtgcggacg ccgggaccgg cggttgtagc
     1801 gcggctggtg cgccgcgatc agccgcagct cccgcaccgc cgcctcgagg gcgtgggcgc
     1861 agacgacggt gtccacccgt tcggcgagcg ccaccatgtc gcgcacccgg cggcgacgct
     1921 ccccggaggt gaagtagctg cgtacccgcc gctgcaggtc gccgctggtg ccgacgtaga
     1981 gcacctcgtc gcgcgcgcct cggaacaggt acacccccgg cgccgccggc acgccggccg
     2041 ccagcacgcg cttcttccgc tgctgctggg tcggccggtg ctgcgccgac tcgcgggcca
     2101 gcccgaggag ctcctcgagg ctctgcacgc cgaggttgcc cacccgttcg agcagatggt
     2161 gcagcacctc gacggtggcg cgggcgtccg caagcgcgcg gtgggtgggc ttcgtggtcg
     2221 taccgaacag ctgagccagc gcgccgagcc ggacgctcgg tgcctcgtcg ggggaaagga
     2281 cggcgcgggc caggcgtgcg gtgcagagca ccggagggcg gggccatgcc cggccatggc
     2341 gctcacacgc ggcgcggagg aagccggcgt cgaagggggc attgtgggcg accagcacag
     2401 caccgctcag gaactccagc agggtgggca ggactgcgga cagccggggt gcgccggtga
     2461 tcatggcagt ggtgatgccg gtgagcgcca cgatgcccgg cggtacgccg gtgccggggt
     2521 cgacgagcgt ggcgagctcg ccgatccgct cgccgccgcg caccttcacg gcgccgatct
     2581 cggtgatcgc gtcgttgcgt gccgagccac cggtggtctc caggtcgagc acgacgaacg
     2641 tcacctcacg cagtggcgtg ccgagctcgt cgaaggacag ctggggggag ggaccgggca
     2701 cggcggcgga cgctagtggc gatcaccgac agaaatccga ctcagatctt gcgcgctccg
     2761 ccaccacatt gtgggtggtg ccgaggtaac gcggataacc tggagcgatg tcagggcccg
     2821 acggtaccgc ctggccgccg gagagcggcg ggaccgggct ggcggctgcc ggtggggcgg
     2881 gcgcgtccga gcccgtcaac tgggagcggc tcccggatgc cgtccgtgcc cggctcgccg
     2941 acatggcggc cgccgcggtg ggggcgatgc ccgctgccga cgtgccggtg tcgctgcgcc
     3001 gtctggcccg cttcactccg gccaagcgcg ccaagctggg tggccaggcc ctgacagccg
     3061 agctggcggc gtccacggcg ttccgcacgg cggtcgtcgc gtggtgggag gagcaccggc
     3121 ccggtgagct gaccgtggcc gcgcacgatg tgctcaccgc ggcggctgcc gcggtgcttg
     3181 ccgacgaccc gtgcgcaccc gaggtcgtgg ctgcggccgc ccgacgcggc gaggccgtcg
     3241 agctgcgtgc tgagcgggat gcggccctct cccgggtgga caagctgacc gtcgagctcg
     3301 accggctgcg cggcgagctg acggaggcgc gggcgcaggc gcgcctggcg ggggagcagc
     3361 gcgacgccga gtaccagcaa ctacggcggc gcgtctcgga acagggtgcg aagctgcggg
     3421 ctgtcgtcga ccagcgggta actgccgagc aggccgcgga ggagctgcgt cgctcggcag
     3481 agagcgacct cgcggttgcc agggcggagc gcgatcgcga gcgcggcagg gccgaggtgg
     3541 agcgcagacg ggccgaccgc gccgcggccg aggtggcggt ggcgcggcag gccgcccgcg
     3601 aggcccgcaa ggccgacgag gtccgtctgg agctgctcat cgagaccctc ggcggtgccg
     3661 tgaagggact acgccgcgag ctggccctcg gcgggggcgg gccgcggcct gccgacctga
     3721 tcgccggtgc ccaggccgcg cgctccgggg cggcgatcga cgggctcgcc gggctcgacg
     3781 cgctgcttgc cgtgccgtcg gtccacctga tcgtcgacgg ctacaacgtc agcaagacgg
     3841 gctatccgga gctgccgctg gccgaccagc gcacccggct cgtcgggcag ctcgctgccc
     3901 tcgccgcgcg cacgggggtc gagatcacgg tcgtgttcga cggggcgggc gtcgtcaccg
     3961 ccccgcaccg ggggtcgcgc ggggtgcgcg tgctgttcag cgaccgcgga gtgctggccg
     4021 acgacgtgat ccgggacctg gcggcggcgg agcctgaggg gcgccctgtg ctcgtcgcca
     4081 cgtcggaccg tgcggtggtc ggatcggtgc gccggttcgg ggcctactcc gtgccatccg
     4141 cggtcctact ggggcggttg gcccggggct gatcagaact gcaggagaaa acttgtcggt
     4201 gggtgctcct aacgtccgcg gcatcgggaa cgtccgaccg cgagggagca gaaatgatca
     4261 tcgattgtga cagctgcgag gtccgcggca acgcctgcgg cgactgcgtg atcggcgttc
     4321 tgctcggcac gccgggcccg cgaacggccg gagtcgagga accggaactg ccgtccggcg
     4381 cgtccatcgt gcaactcgac gcaccggaac ggcgtgcctt ggatgtactc gcggatcaag
     4441 gcctcgtgcc gagattgcgg ttggtggaca ccggcgcgcg ccgagcgtcc acctgttcgg
     4501 gcgacagcgg gcctgctcgt gacgctgtgt gacatcacag cgttgtagaa ctcatttgac
     4561 tgcgctttgc gttcggttgg cgagccgacc cccgcgcgac acgccatgac ccgccgcttg
     4621 gccgcgcttg gcctcctccg taatctccgc gagaccttgt ggttcttcag ccattcgaca
     4681 ggaatggcga cgcaaggtca ccgcacaatc gtccgatcgg tcgctgaata cccccgatcg
     4741 gggagcgtgc gggtggtgag aggagaccat ccgcgtggtg tcgcctcggc gtaccccatc
     4801 cggtcgcggg atccggcgga ttgagttccg gtccatgctt cgccggcgtt gcgcgccatc
     4861 ggccggagcc ctcgtcctcg cctcgctcct cgccctcgtc gtcggtgtcg cgccggccag
     4921 tgcccagtcc gcacccccgc caccgccccc acctccgacc aatgcagccg atgctgccgc
     4981 gcaactcaat caggtgcagc acgacgcgga ggccctcacc gaggagtggc acgcggccca
     5041 ggacacggtg accgcccgcc aggccgagct ccagacgctg caggccgctg tcgcgcccgc
     5101 gaaggccgcc gtcgatacgg ccaaggccag cgaggagcag ttccgccagc aggtcgacgc
     5161 gctcgcgatg ggcacgttcg agagcggcaa cctcgaccag ttcaacgcgc tgctcgcgag
     5221 cgggtcaccg caggacttcc tggaccagat gtcggctctg gagacactct cggccgacta
     5281 caaggacgcg ctcgcgcagc tgcaggccgt cgtcgacaag acagccaagg cgcagtccga
     5341 cgccgacgac gccgtcgccc gcgcacagac cgctgctgac gaggctgcca aggccgagca
     5401 ggacgtcgcc gctcgcaagc gcgatgcgga gatgcgtatc gaccaggccg agcagctcct
     5461 caagcagctg tcaccccagc agcggcgcgc ccgcaacggc ccggacgtga gcgctccgag
     5521 cggtcccatc accggcactg gcgtcggggt cgaggccctc cgtgcggcgg caacccagct
     5581 cggcaagccc tacaagtggg gcgccgaggg ccccaatagc tacgactgct ccggcctcac
     5641 gagctgggcg ttcaagcggg ccggtgtcac gctcccgcgg tcgagcagcc agcaggcgcg
     5701 ggtaggcagc tccgtgtcgt gggacaacat gcggcccggc gacctcgtct tctactactc
     5761 gccggtgagc cacgtcggca tctatgccgg cggcgggaag atgatcgacg cgccgcagac
     5821 cggcgacgtc gtgaagtacc agacggtgtc gagcagcgcc ttcagtggcg cccgccgcct
     5881 ctagaggacc tcgtccgctc cccgaaacgg gcagcggccg gccgggcgac cggccggccg
     5941 ccgcccgacc tccacgccgg ccggacgtcc gtccacccga gcctggacta gcgtcgctcg
     6001 ggtgccgtcc acgttgctgg tgacgaacga ctttccgccc cgcaccggcg gcatccagag
     6061 ctatctccag gcgttcgccg atcggctccc gcccggtgac ctcgccgtgt acgcgccggc
     6121 ctggccgggc gctgcgcact tcgacgccgc ccgcccgtac cccgtgcacc ggcacccgac
     6181 ctcgctgatg gtgcccaccc ccggcgtcgc ccggcgagca gctgcgctgg cgcgcgagca
     6241 cggtgcgagc accatctggt tcggcgcatc ggccccgctg gcgctgctgg gtccggggct
     6301 gcgccgcaag gccgggatcg cgcgcctcgt ggccagcacc cacggccacg aggtcggctg
     6361 gtcgatgctg cccggcgccc ggcaggcact ccgccggatc ggctcgcaca ccgacgcgct
     6421 caccacgatc tcgcagtaca cccgcggccg ggtcgcggca gcgttcggcc cgtgcgccgc
     6481 gctggaggtc gtgccgtcgg ggatcgacgc cgaagtcttc cggcccgaca ccgccgctcg
     6541 tgtcgcgctg cgccgccgct atggcctcgg tgacgctccg gtcgtcagct gcgtgtcccg
     6601 gctcgtcgcg cggaagggac aggacgcgtt gatcagggcg ctgccccggg tgcgcagcca
     6661 ggtgccggga gctcgtctgt tgctggtcgg aagcgggccc tacgccgacc ggctgcgccg
     6721 gctggcggtc gacgccggcg tggccgagca cgtcgttttc accggcgccg tgccggcagc
     6781 ggagctcccg gcacaccacg ccgtcgggga cgtgttcgct ctgccgtgcc gcacgcgggg
     6841 cggcgggctc gacgtggaag ggctgggcat cgtcctcctg gaagcggccg ctgcagggtt
     6901 gccggtggtg gccggagact cgggcggtgc tcccgagacc gtgcaggacg gccgcaccgg
     6961 tcacgtcgtc ggcggccggg acctcgacgc gctggtcgaa gccgtggccg gcctgctcgc
     7021 ccacccggac cgggcgcggg cgatgggcgc ggcgggccgg gagtggatgc tgcgcgactg
     7081 gacctggccc gcacgggttg cccggctgag cgccctgctc acgggctccc ccatatcggc
     7141 accggcctga ctgccagccc cgacagcccc cacccccgcc ggcacccgcc gcgcacgaga
     7201 tggacacatg tcgctgttcg gcggcggcct cgatccacga cacgtatcca tgtcgcgccc
     7261 gtgacgccgg cccgggaggg gcgtgggaat gctggtcagg tgggggactt cgttgtgctg
     7321 tagatgctcg cgatgtcgcc cgcgaaggtc ttcgccacca cggcgcgctt gaccttcatc
     7381 gtcggggtca tctcgccgcc tgcctcggtg aagtcgacgg gcaggatgcg gaactcgcgg
     7441 atctgctcgg cacgggagac ggccttgttg gcctccgcga cggcgttgcc gatctcggcg
     7501 cgcaggtcgg ggtcgtccac gaggtcggcc gcgccgtcgc ccgccggctt gccgttgcgt
     7561 tcgcgccagc cgggcagggc gtcgccgtcg atcgtcacca gtgccgcgat gaacggctgc
     7621 gcgtccccga ccaccatgca ctggctgacc agcggatgcg cgcgcagccg gtcctcgagt
     7681 actgcgggcg cgacgttctt gccgcccgcc gtcacgatga tctccttctt gcggccggtg
     7741 atcgagagga agccggcgtc gtcgacctcg ccgaggtcgc cgctgtggaa ccagccgtcc
     7801 tcgagcgcct ctgcggtggc ggtctcgttc ttccagtacc gatggaagac gaccgggccc
     7861 ttcagcagga tctcgccgtc gtcggcgatg cgtacggagt ggccggacac gggacgcccg
     7921 accgagccga tccgctgagc cgtgagtgtg ttgatcgtga tcgccgcgct ggtctccgtg
     7981 aggccgtagc cctcgaggat gggcaggccg atcccccgga agaaatgtcc gagccgatcg
     8041 ccgagcggtg cgcttccgga acccgccgcc acgacgctgc cgccgacggc ggcgcgcagc
     8101 ttgccgtaga cgagcttgtc gaacagcgcg tgccggagct ggaggccgag ccccgggcca
     8161 cccgtgtcgc gagcccggct ccacgcgatc gcggtgtcgg cggccgcgtc gaagatgcgt
     8221 cccttgccgt cgtcgtgggc gcgctggcgg gccccgttgt acaccttctc gaacacgcgc
     8281 gggacggcga gcaggaacgt cggactgaac gcctgcaggt cgggcagcag ggtggacacg
     8341 tccggcgtgt ggccgatgac ggtgcgggtg gcgacggcgc agtactggag cgcgcggccg
     8401 aacacgtgcg cgagcggcag gaacagcagg aacgacgcgc ccgccgtgag tacctgcggg
     8461 aaatcctgcg ccaacccctt ggcctcgaag agcaggttgc ggtgggtgag ctcgcagccc
     8521 ttgggacggc ccgtggtgcc ggaggtgtag atcaggctgg ccaggtcgtc ggcgcgtacg
     8581 gcacgccgcc gggcgtgcac ctcgtcggcc ggggtgtcgc tgcccagagc ggcgagctgc
     8641 tcgatcgccg ggggtgtctc cgggccggcg gcagccccgt cgatctgcca cacgtgccgc
     8701 agtgccggga tgtcggccga gacgctctcc accagcgccc ggtgcttgtc cgtctcgacg
     8761 acgacggcga cggcctcgga gtcggtgagg atccagttga tctgttccgc agaggacgtc
     8821 tcgtagatcg gcacggtgac gccgccaatg gccagcacgg cgtagtcgat cacggcccac
     8881 tcgaagcggg tgcgggacag gagggcgacg cggtcgccgg gctcgacccc ggccgtgatc
     8941 agcccacgcg ccactgcagt gacctcgtcg gcgaactgcc ccgcggtcac ctcggaccat
     9001 gtgccgtcgc tgctgctgct ccggcggcgg aaggccggcg ccgacgcgtc ctgggcggca
     9061 ttgtcataga cggcgccgac gaggttgtcc tcgtcggcga cacggaacgt tgcaggcacg
     9121 ctgtactcgc gcacggtcat gacctcctgg ccagcgctgg ctgtggttca gcgcaaccta
     9181 ccgtgcagta cggagtgcag cgagtgccga ctatgccggg ccctcccgct ccggcaggct
     9241 gtctccatgc cgtcggtgga cgtggtggac gagacctttc tcgccgtggc gcccgaggtg
     9301 gtcgccgccg agttcgcgca acccgtgcgg tggccgcagc tgtggcctga cctgcgcctc
     9361 gaggtcatgg ccgatcgtgg ggtccagggc gtgcgctgga cggtgcaggg ggcgctcgtc
     9421 gggagcatgg aggtctggct ggagcccgtg ctcgacggga cggtgctgca ctacttcctg
     9481 cgggccgacc cggccggacc ggacggcacc ccgtgccccg catcggcgcg gcgcggtgcg
     9541 gcggaggcgc gtcgacggca gcgtgcggcg aaggccattg cattcgcatg caaggaccgg
     9601 ttggaggcgg ggcgcgcgcc cggcgagcca ccgacgtaaa ttgatgacca tgcgtgtgca
     9661 cgtggtctcc gacgtgcacg gcaacgtcga agcgctggcc cgggccggcg atggcgcgga
     9721 cgcgctggtg gtgctgggcg acctcgtcga ctacgtcgac tacggcgctc ccgaggccgg
     9781 catcctcgga cgggtcctcg gtcccgacgt cagcaggcgt ttcgcgcagc tgcggaccaa
     9841 cggggcaccc ggtgagctcg tcgactacgt ccgcggggcc tggtcacggg tggaggacgc
     9901 ggcggtcgtc gtcgagcagg ccgtccggga gcagtacgac cggctgttcg cggcgatgac
     9961 cgctccgacc tacgcgatcc cgggcaacgt cgacctcccg cggctgtggc ccgggtacgc
    10021 gcggcccgga ctctgcctgc ccgacggcca ggtcgtcgac gtgggcgggc tgcggttcgg
    10081 cttcgtcggc ggggcaccac tgcctcctgg gcaggcgccg cgccgcggcg gtccctggac
    10141 gccccacctg cgtctcgccg aggagttcgc cgccgcggtg cacggtctcg gcccggtcga
    10201 cgtgctgtgc agccacgtgc cgcccgcggt cccggaactg gccttcgacg tggtgtcgcg
    10261 gcgcccggag gcggccgcgc ccgcgctgct cgacatcatc caccgggacc ggccgcgggc
    10321 ggccctgttc gggcacgtcc accagccact ggccgcccgg gcgcgggtgg ggcgaaccga
    10381 gtgcatcaac gtcgggcact tccgccggac gggcgtgccc tacgtgctgc gctggtgatc
    10441 catcgatccc ggagggtgtc gatgttcggg cctgcaaccg acccttcggt aacctgccgc
    10501 catggccgac gctacgacct ccacgatcac catcgccgcg gcgccggagc gcgtgatggc
    10561 ggtgatcgcc gacttccctg cctacccgga gtgggcctcg caggtgaaga gcgtcgaggt
    10621 gctcggcagc gacgcccagg gccgcgccga gcgggtgaag ttcaccatgg acgcgggccc
    10681 gatcaaggac tcctacacgc tcgactacac ctgggcctcc gatgcgaggt cggtgagctg
    10741 gacgctggtg aagggccaga tccagaaggc gcagaccggg tcatacacgg tggagggcac
    10801 cgggtcggag accaccgtca cttacagcct ggcggtcgac ctgaacatcc cgatgatcgg
    10861 gatgctgcgg cgcaaggcgg agaaggtgat catcgacact gcactcaagg ggctgaagcg
    10921 acgtgtcgag agcgccccct gacccatcgt ccctgatccc gcgtctaccc tccgcgtcgt
    10981 gcgcgtcgtc cttttcaccg gcaagggagg cgtcgggaag acaactctcg ccgcggccac
    11041 ggccacgatg ctcgcgcgct cgggccgcaa agtgctggcg gtctccaccg atcccgccca
    11101 ctccctcggc gacgcgctgg aggcggatct cggcggcgag ccagtggagc tcgaggctgg
    11161 gctctgtgcc gcgcacatcg acaccagggc gctgctcgac gactcgtggg gacggctgca
    11221 ggtgcacctc cgcacgatgc tgtccggcgc aggcgtcgac gagctggtgg ccgacgagct
    11281 gacggtgctg cccggcgtcg aggagctgct cgcgctgggt gaggtgcgcc gagccgccga
    11341 gacggggctc tgggaggtcg tggtcgtcga ctgcgggccc acggccgaga cgctgcggct
    11401 gctggcgctg ccggaggctt tgtcgggcta cctggagcgg ctgttccccg cccatcggcg
    11461 cgccgtgcgc ggcctgctcg ccggtctggc cggagccgga cgcgatgcgc agcctgcctg
    11521 ggaccgtacg atcgacgcgc tcgacgggct ggccgagcag ctcaccggtc tccgctcgat
    11581 gctcgccgac cacaccagga cctcgatccg gctcgtgctc accccggagc gggtggtggt
    11641 cgccgagacc cggcgcaccc tcaccgcgct cgccctgcac gggctgcacg tcgacgcgct
    11701 ggtcgccaac cgggtgctac ccgggccgcc gccctcgctg cgaggccccg cagccaggtg
    11761 gctgcgcgaa cggcacgccg agcaacaggt cgtgctcgcg gagctgggcg cggcaccggt
    11821 gccgctgcgc accgtcgcct acaccgccgc cgagccgacc ggcgtcgcgg cgctgctggc
    11881 ggtggcgcag tcgctgtacg gctccaccga ccccgctcca ccggcaggcc ccgcgaccgc
    11941 cccgctgctg caggtgcggc gaaccgccgg cagcggtacc tcgctcgact ccgagttcga
    12001 gctcgtgctg cggctccccg gcgtcgctgc cgggtcgctg gacctcgccc gtgtcgagga
    12061 cgagctggcc gtcaccgcgg gcggcacgcg ccgcatggtt gcgctgccgt cggtgctgcg
    12121 ccgctgcacg gtgaccggcg cccgcctgtc gggcgacgac ctctgtgtgg tgttcacccc
    12181 ggacccgtcg gtctggctcc ggtgacggcg accccgggag gctgtggcga gcacgccggg
    12241 ctcagcgcgg agctgcgcgc gctcgccctg agcgcgctcg accggctcga acccgtgctc
    12301 gagcggatgc gtaccgagcc gaccgcgcgg accgccgaga cctgcacggc gtgccccgtg
    12361 tgtgtgctga tcgcggcgct acgcggggag caccccgacc tcgccggccg gctcgccgag
    12421 cacgccaccg ggctcgtggc ggtgctgcgg gctgcgctgg aggagggcgt acccgcgagc
    12481 gagagctccc cggcgccgga acccgaaccg gccgcgcctg cacgtcaggt ccagcacatc
    12541 ccgatcgacc gtgggccggc ggtcggatga ccgccagcag gggggcctcc cggttggcaa
    12601 catctccccc cgtgctgacc gtgggagtcg atgtcggggg cacgagcgtg cgcgccggcg
    12661 tcgtcgacgc ggaggggaac gtccacgaca cggcccgcac cccgacgcca cgcagcgagg
    12721 ccgcgctgga ggccgccctc gcgggcgtca tcaacgagct ggccgcccgg caccggatcg
    12781 gtgcggtcgg gctcgccctg gccggcttcg tcactcccga ccggcgcggg gtccggttcg
    12841 ccccgcacct gtcctggcgc gatgcccccg tcgccgaccg gatcgccgag cgtgtcgggc
    12901 tgcccgtcgt cgtcgagcac gacgccaacg cggcggcgct cgccgagcgt cac
//
LOCUS       DASRSC010000002         4076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2130539,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000002 DASRSC010000000
VERSION     DASRSC010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2130539"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="VFE39_00070"
     CDS             <1..1155
                     /locus_tag="VFE39_00070"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET6257023.1"
                     /translation="AQMATPELVSFMVRYTSGYICVALTEEDCDRLDLPPMYHSNGDA
                     FRTAFTVTVDAKAGITTGISATDRAHTIRLLADPTAGPADLVRPGHVLPLRAREGGVL
                     RRPGHTEAAVDLARLAGLSAAGALCEIVSQKNEGEMARGDELRVFAEEHDLTLITICD
                     LVAYRRRFEKHVVRVAQARIPTAHGDFIAYGYDSLLDGIEHIAMVRGDVATPESTGED
                     VLVRVHSECLTGDVLGSMRCDCGPQLEAALAAVAAEGRGIVLYMRGHEGRGIGLLHKL
                     QAYQLQESGVDTVDANLALGLPADARDYGIGAQILVDLGVKSMRLLTNNPDKRAGLEG
                     YGLTITGRVSLPVRASRENLRYLRTKRDRMGHDLPGLDDTGPIVADGTGT"
     gene            1172..1654
                     /gene="ribH"
                     /locus_tag="VFE39_00075"
     CDS             1172..1654
                     /gene="ribH"
                     /locus_tag="VFE39_00075"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET6257024.1"
                     /translation="MSGEGRPEHQEIPDGSGLRVAVAAATWHADIVDALLERALETAG
                     KAGVENPTVVRVPGTVELPVVCQELAGSHDAVVALGVVIRGGTPHFEYVCDAVTAGLT
                     RVALDERTPVGNGVLTCDTRAQAVDRSGGQGSTEDKGAEACLAALHTAVVLRDLRSRS
                     "
     gene            1738..2163
                     /locus_tag="VFE39_00080"
     CDS             1738..2163
                     /locus_tag="VFE39_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET6257025.1"
                     /translation="MVVRPRKVLIAAWVGSVAVVVLFVVVAVLLRNTQTGVYFRFADQ
                     VALVLMGLLVGGGVLLLARPRVRADAEGIEVRNILTTKRLPWGLVRRVAFPDGASWAR
                     LDLPDDEYLPVLAIQAVDGFRAVEGIAKVRALHAAARTS"
     gene            2212..3243
                     /locus_tag="VFE39_00085"
     CDS             2212..3243
                     /locus_tag="VFE39_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET6257026.1"
                     /translation="MLRARDQVDQDPSGDESVRLGDIVLRNRLVTSSSLLGYGVSNSP
                     LVPYGMSPVSLFVPLAEFGAVTTRTLTVEPREGHFSTREDYALHELPGLLKRYGTVLR
                     GTDGGWLNAFGWCNVGIDAYLRDYYPRTRDQRVIISLGGFSAEEFVTLVDRVNAAVPA
                     GDIAAVEFNVSCHNVNFDFSTILQDVLDEAVPRSNHPVILKLSPDYDYLRNADQAAKA
                     GVSALTAINTVKALRLDPRTGKPWLANRYGGLSGRAIKPIGLRVVSELREAGVTLPII
                     GTGGIRTVDDCREYFWAGADAVSLGSATWLASYPGYALAPLRALHVRRVLKAVRRYRA
                     LPSRSSVSR"
     gene            3428..>4076
                     /locus_tag="VFE39_00090"
     CDS             3428..>4076
                     /locus_tag="VFE39_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET6257027.1"
                     /translation="MEGGNQLDRVRAPDRRPRVVLIDVFETMLQVDALRARFVDVGRP
                     EHEWELFFTRTLRDGMALTLAGGVRPFADVARAALRTTSHHTLSEEALDHVLAGFREL
                     PPHPDVEPALRALARARIPSYAFTHGSAEVASDALDRAGLRTYLRGVLSTEEIRSFKP
                     PARAYNWACQQVDAPGDRVALVAVHSWDVHGAVRAGLVGGFSTRLEGVVPDVVARP"
BASE COUNT          577 a         1402 c         1502 g          595 t
ORIGIN      
        1 gcgcagatgg cgacgccgga gctggtgtcg ttcatggtgc gctacacctc cggctacatc
       61 tgtgtcgcgc tgaccgagga ggactgcgac cggctcgatc tgccgccgat gtaccactcc
      121 aacggcgacg ccttccgcac cgcgttcacc gtgacggtgg acgcgaaggc gggcatcacc
      181 accggcatct cggccacgga ccgggcgcac acgatccggc tgctcgccga cccgacagca
      241 ggtcccgccg acctggtgcg gcccggtcac gtcctgccgt tgcgcgcccg tgaaggcggc
      301 gtgctccgca ggccaggaca caccgaggcc gccgtggacc tggcccggct cgcgggcctg
      361 tcggccgcgg gtgcgctgtg cgagatcgtg tcgcagaaga acgagggcga gatggcgcgc
      421 ggcgatgagc tgcgggtgtt cgccgaggag cacgacctga ccctgatcac catctgtgac
      481 ctggtcgcgt accgccgccg tttcgagaag cacgtggtgc gcgtggcgca ggctcgcatc
      541 cctaccgccc acggcgactt catcgcctac ggctacgact ctctgctcga cggcatcgag
      601 cacatcgcga tggtgcgcgg cgatgtggcg acgccggaga gcacgggcga agatgtgctc
      661 gtgcgcgtgc actccgagtg cctgaccggc gacgtgctgg gctcgatgcg ctgcgactgc
      721 gggccgcagc tcgaggccgc gctcgccgcg gtggccgcgg agggccgggg gatcgtgctc
      781 tacatgcggg ggcacgaggg caggggcatc ggcctgctgc acaagctgca ggcctaccag
      841 ctgcaggagt cgggcgtcga caccgtggac gccaacctcg cactcggctt gcctgcggac
      901 gcgcgcgact acggcatcgg tgcgcagatc ctggtggatc tcggcgtgaa gtcgatgcgg
      961 ttgctcacga acaacccgga caagcgcgcc ggcctggagg gctacggcct gaccatcacc
     1021 gggcgcgtgt cgcttccggt ccgcgcgagc cgtgagaacc tgcggtacct gcgtaccaag
     1081 cgcgaccgga tgggccacga cctgccgggt ctggacgaca ccggcccgat cgtggcggac
     1141 gggacgggaa catgaacgcg gtgggcacga ggtgagcggc gaggggcggc cggagcacca
     1201 ggagatcccg gacggcagcg gattgcgcgt ggcggtggcc gcagcgacct ggcacgccga
     1261 catcgtcgac gccctgctgg agcgggcgtt ggagaccgcg gggaaggctg gtgtggagaa
     1321 cccgacggtc gtgcgcgtgc cgggcaccgt cgagctcccc gtggtgtgcc aggagctcgc
     1381 cgggagccat gacgccgtgg tggcactcgg cgtggtgatc cggggcggca ccccgcactt
     1441 cgagtacgta tgcgacgccg tgaccgccgg gctcacacgc gtggcgctcg acgagcgcac
     1501 gccggtgggc aacggagtgc tcacctgcga cacccgtgcc caggccgttg accgctccgg
     1561 cggccaggga tcgacagagg acaagggcgc ggaagcctgc ctggcagcgc tgcacaccgc
     1621 tgtcgtgctc cgcgacctgc ggagcagatc gtgaccgggc cccctgatgg caccgaccaa
     1681 ggcactggga accgggcgga taccggcacg gggaccggac cggccggcgg cacgagggtg
     1741 gtggtgcgcc cgcgcaaggt gctgatcgcg gcgtgggtgg gttccgtggc ggtcgtggtg
     1801 ctgttcgtcg tggtcgcggt gctgctgcgc aacacccaga ccggcgtgta cttccggttc
     1861 gccgaccagg tcgccctcgt gctgatgggt ctcttggtgg ggggcggagt gctcctcctc
     1921 gcgcgcccgc gggtacgggc cgacgccgag ggcatcgagg tgcgcaacat cctcacgacc
     1981 aagaggctcc cgtggggcct cgtgcggcgt gtggcgttcc cggacggggc gtcctgggcg
     2041 cggctggacc tgcccgacga cgagtacctg ccggtgctgg ccatccaggc cgtcgacggc
     2101 ttccgcgccg tggagggaat cgccaaggtg cgggcgctgc acgccgccgc ccgcacctcc
     2161 tgaaccccgc ggaccggcat cccgcgccgg gccgcacgta gcatcggggc cgtgctgcgt
     2221 gcccgtgatc aggtagacca ggatccctcc ggcgacgaga gcgtccgcct gggggacatc
     2281 gtgctgcgca accggctggt cacgtcctcg agcctgctgg gctacggggt gtcgaactcc
     2341 ccgctcgtgc cctacggcat gagccccgtg tcgctgttcg tgccgctcgc cgagttcggt
     2401 gccgtgacga ccaggacgct gaccgtcgag ccccgcgagg ggcacttcag cacgcgtgag
     2461 gactacgccc tccacgagct ccccggcctg ctgaagcgct acgggacggt gctgcgcggc
     2521 accgacggcg gctggctcaa cgcgttcggc tggtgcaacg tggggatcga tgcctacctg
     2581 cgcgactact acccgcgcac ccgcgaccag cgcgtgatca tctccctcgg cggcttctcc
     2641 gccgaggagt tcgtgaccct cgtggaccgc gtcaatgccg ccgtgccggc cggtgacatc
     2701 gcggcggtcg agttcaacgt ctcctgccac aacgtcaact tcgacttctc cacgatcctg
     2761 caggacgtgc tcgacgaggc ggtgccccgc agcaaccacc ccgtgatcct gaagctgtcg
     2821 cccgactacg actacctccg caacgccgat caggccgcca aggcgggggt gtcggcactg
     2881 accgcgatca acaccgtcaa ggcactgcgg ctggacccgc ggaccgggaa gccgtggctg
     2941 gcgaaccgct acgggggcct ctccgggcgg gcgatcaagc cgatcgggct gcgtgtggtg
     3001 agcgagctcc gcgaggccgg ggtgacgctg ccgatcatcg gcacgggcgg gattcgcacc
     3061 gtggacgact gccgcgagta cttctgggcg ggcgccgacg cggtgagtct cggaagcgcg
     3121 acatggttgg cgagctaccc cggctacgcg ctcgcgccgc tgcgggcgct gcacgttcgg
     3181 cgcgtgctga aggctgttcg gcgctacaga gcgcttccgt cccgctcctc tgtgtcgcgc
     3241 tgagcgacga tcttcacccg taagattcgg gcagtgcccc agttggagcc ggcccgattt
     3301 cccgagtgga agccgatggc ctcagcgcga tgagtgacag cacgatgaac ggcaccagga
     3361 gcgacagcac ggtgaacccg gcccggtccg acggacaggg catgacagac agggcacgat
     3421 gaccccggtg gagggcggaa accagctgga ccgcgtgcgg gcgccggacc gcagaccccg
     3481 tgtggtgctc atcgacgtgt tcgagacgat gctgcaggtg gacgcgctgc gcgcgcgctt
     3541 cgtggacgtg gggcggcccg agcacgagtg ggagctgttc ttcacccgga cgctgcgtga
     3601 cggcatggcc ctcacgctcg ccggcggcgt tcggccgttc gccgacgtcg ccagggctgc
     3661 gctgcgcacc acctcgcacc acacgctgtc ggaggaagcg ctcgaccacg tgttggccgg
     3721 cttccgcgag ctgcccccgc acccggacgt ggagccggct ctgcgggcgc tcgcacgggc
     3781 gcggatcccg agctacgcct tcacccacgg ttcggcggag gtggcgtcgg acgcgctgga
     3841 ccgtgccggg ctgcgcacct acctgcgggg cgtgctctcc acggaggaga tccgctcgtt
     3901 caagccgcct gcccgcgcct acaactgggc gtgtcagcag gtggatgctc cgggagaccg
     3961 ggtggcgctc gtggcggtgc actcctggga cgtgcacggc gccgtgcgcg cagggctcgt
     4021 gggcggcttc tcgacccggc tcgagggcgt ggtgcccgac gtcgtggccc ggcccc
//
LOCUS       DASRSC010000003         5420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4914537,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000003 DASRSC010000000
VERSION     DASRSC010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4914537"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFE39_00095"
     CDS             <1..735
                     /locus_tag="VFE39_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET6257028.1"
                     /translation="QLLGPRDGSPQEPPEDPEADDPEGWAADVDVLLAERAAARARPT
                     VALPPQLSVTQLVELAADPAGLAARLRRPLPLPPNPHARRGTAFHAWLEQRFGAAQLL
                     DLDELPGAADEGAAPDDALTELQDAFVTSAWAERRPVEIEVPFETVVAGVGVRGRMDA
                     VYADPDGGFTVVDWKTGALPDETRMPALAVQLAAYRLAWAALAGCAPEQVRAAFHFVR
                     HDITLRPADLLDAAGLRELVMSVPVA"
     gene            complement(745..1278)
                     /locus_tag="VFE39_00100"
     CDS             complement(745..1278)
                     /locus_tag="VFE39_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET6257029.1"
                     /translation="MPRYVALLRGINVGRAKRVAMADLRELLGELGYTAVRTHLNSGN
                     VVFTGDDAAPVEHRERIEAAITDRLGLDVRCVVLTSDDLCAIVDGHPFADVATDGSRM
                     MAHVLAEAPDPALLAEYDPIALDPDNSRLGERVIYQWCPDGVLAAPPVGGFAQKHLGV
                     PVTGRNWNTMTKLAQLI"
     gene            1388..2500
                     /locus_tag="VFE39_00105"
     CDS             1388..2500
                     /locus_tag="VFE39_00105"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HET6257030.1"
                     /translation="MSARIVLFGATGYTGGRTAEAMAARGLQPVLAGRDPARLAAAAE
                     RLGGLETARADVTDEGSVAALVGPGDVLVSTVGPFVKLGRPAVSAAVDAGAIYLDSTG
                     EPPFIREVFERRGPAAERTGATLLTAFGNDYVPGVLAGALALRAAGEGATRVDVGYFI
                     TGAGRGQAFSRGTLRSVIGVATEQVYAWRDGALRTEPAGARLRTFDVAGRPRPGVTIG
                     GTEQFALPRLAPGLRTADVYLGWFGAASHAVHLGSRVTPWLGRVPGTSQALAAMAGLL
                     TRTATEAPSAEALAGMRSHFVAEVFDDTGTLLARTRLTAPEAYTITADLLAWGAGRAA
                     EHGVRGSGALDPVAAFGLDELSAGAAAAGIVPADGS"
     gene            2706..3704
                     /locus_tag="VFE39_00110"
     CDS             2706..3704
                     /locus_tag="VFE39_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HET6257031.1"
                     /translation="MPDSSASPLVSLLRRLAIAFGALAAAALVVYFGGNGYVDNNSNT
                     PISLGDAFYYATVSLSTTGYGDIVPVTPEARLLTTVVITPLRLLFLIVLVGTTVELLT
                     ERSRQGVRIERWRSRVRNHTIVVGYGTKGRAAVETLLGDGAAPTDIVVVDTDRTQLDA
                     ASALGLVTVTGNATRSSVLRIAGLQQAAAIVVATNRDDTAVLVTLTARELAPDVRIVA
                     SVRESENVHLLRQSGANSVIVSAETAGRLLGMATTTPSVVEVVEDLLTPDAGFAISER
                     PVEDSEVGGSPRHLADIVLGVVRGDELFRVDASAVDALERGDRLLYVRKASLPDED"
     gene            complement(3853..5241)
                     /locus_tag="VFE39_00115"
     CDS             complement(3853..5241)
                     /locus_tag="VFE39_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6257032.1"
                     /translation="MRSAGRLRTVSLRRRVTALSMLVLAAVLVLVGVLTDVVFATQTR
                     AHLHDELTVRAALADRLVTEGLPTPEVVRRVEAPGIRAVVVAADGAVYDGGEPERAPG
                     PPWRQGPGDYTGRDGWGDGSGAGPGRSDQVLQQPLVNGARLTLVADGAQVTAAQRLLR
                     RTLLLLSIAALAVAGVVTLLTTRVALAPLDAMTALARSITRGDRGRRLAPSRTDTELG
                     RTASAFDAMLDELEGAEASARASEARTRQFVADAAHELRTPLAGVQAAAEAAIAPGLD
                     AEERDRLHLLLLRESHRAGRLVDDLLALARIDAGIELRHEPVDLLELARAEAERVRLL
                     APNRTVEVRGDSAVVPGDPARLGQVLGNLLDNARRYTPSGGRITVGVSVRGAATLLVT
                     DTGPGVPPADRERIFDRLVQLDEARTADGGGAGLGLAIARGIARAHGGDLRCVDPPDG
                     TGAAFALILPIS"
     gene            complement(5238..>5420)
                     /locus_tag="VFE39_00120"
     CDS             complement(5238..>5420)
                     /locus_tag="VFE39_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HET6257033.1"
                     /translation="VSKGQILTRVWGYTDYDPNLVEVHVSALRRKLGEPRLVHTVRGL
                     GYVLRAGGDGGPETPA"
BASE COUNT          695 a         2002 c         1980 g          743 t
ORIGIN      
        1 cagctgctgg gtccccggga cggatcgccg caggagccac cggaggaccc ggaggccgac
       61 gaccccgagg gctgggccgc ggacgtcgac gtgctgctcg ccgagcgcgc cgcggccagg
      121 gccaggccga ccgtcgcgct gccgccgcag ctctcggtca cccagctcgt cgagctcgcg
      181 gcggaccccg ccgggctcgc cgcccggctg cgccgcccgc tcccgctgcc gcccaacccg
      241 cacgcccgtc gcggtaccgc cttccatgcc tggctggagc agcggttcgg tgcggcgcag
      301 ctgctcgacc tcgacgagct gcccggagcc gccgacgagg gcgccgctcc cgacgacgct
      361 ctcaccgagc tgcaggacgc cttcgtcacc tcggcgtggg ccgagcggcg acccgtcgag
      421 atcgaggtgc cgttcgagac cgtggtcgcg ggtgtcgggg tgcggggccg gatggacgcg
      481 gtgtacgcgg acccggacgg cggcttcacg gtggtcgact ggaagaccgg cgcgctgccc
      541 gacgagacgc ggatgccggc tctcgcggtg cagctggcgg cctaccggct ggcctgggca
      601 gcgctggcgg ggtgcgcgcc ggagcaggtc agggcggcgt tccacttcgt gcggcacgac
      661 atcacgctgc ggccggccga cctgctcgac gcggcagggc tgcgggagct cgtgatgtcc
      721 gttcccgtgg cgtagtgccg aggcctagat cagctgggcg agcttcgtca tcgtgttcca
      781 gttgcggccg gtgaccggga cgccgaggtg cttctgcgcg aacccgccca ccggcggtgc
      841 agcgagcacg ccgtcgggac accactggta gatcacacgc tcacccagcc gcgagttgtc
      901 cgggtccagc gcgatcgggt cgtactcggc gagcagcgcg ggatccggcg cctcggcaag
      961 aacgtgcgcc atcatccgcg acccgtccgt cgccacgtcc gcgaatgggt gcccgtcgac
     1021 gatcgcgcag aggtcgtcgc tggtcaggac gacgcaccga acgtcgagcc cgagcctgtc
     1081 ggtgatggcg gcctcgatgc gttccctgtg ctccaccggg gcggcgtcat cgccggtgaa
     1141 caccacgttc ccgctgttga gatgcgtgcg gaccgccgtg tagcccagct cgccgagcag
     1201 ctcacgcagg tcggccatcg ccacacgctt ggcgcgtccg acattgatcc cgcgcagaag
     1261 tgcgacatac cggggcacgc gggcacggta ccggtgatcg tcgcgagaac cctcgatccg
     1321 ccctcggaag ggcggttgtc gggatctcgg cgcgatcttc gcccttgctg gtggatagtg
     1381 tcctggtgtg agcgcgagga tcgtcctgtt cggtgccacc gggtacaccg gtgggcgtac
     1441 ggccgaggcg atggcggcgc ggggcctgca acccgtgctc gccggccggg accccgcccg
     1501 gttggctgct gccgcggagc ggctcggcgg tctcgagacg gcccgggcgg acgtcaccga
     1561 cgagggttcg gtcgccgcgc tcgtcgggcc gggggacgtg ctggtcagca ccgtcggacc
     1621 gttcgtgaag ctcggccgcc ccgcggtgtc cgccgctgtc gacgcgggcg ccatctacct
     1681 ggactccacc ggcgagcccc ccttcatccg ggaggtgttc gagcggcgcg gacccgctgc
     1741 ggagcgaaca ggggcaacgc tgctcaccgc gttcggcaac gactacgtgc ccggtgtgct
     1801 ggcgggcgcg ctggcgctgc gggcggcggg ggagggcgcc acgcgggtcg acgtcggcta
     1861 cttcatcacg ggtgcgggca ggggccaggc gttctcgcgc gggacactcc gttcggtgat
     1921 cggcgtggcc acggagcagg tctacgcctg gcgcgacggt gcactgcgca cggagccggc
     1981 cggcgcccgg ctccgcacct tcgacgtggc cggcaggccg cgcccgggtg tgacgatcgg
     2041 cggcaccgag cagttcgcgc tgccgcggct cgcgccgggg ctgcgcacag cggacgtcta
     2101 cctcggttgg ttcggcgcgg cgagccatgc ggtgcacctc gggtcgcggg tcacgccatg
     2161 gctcggccgg gtgccgggca cctcgcaggc gctggccgcg atggccgggc tcctgacccg
     2221 cacggcgacg gaggcgccgt cggccgaggc gctggcgggg atgcggtccc acttcgtggc
     2281 cgaggtgttc gacgacaccg ggaccctgct cgcccgtacc cgcctgaccg cccccgaggc
     2341 gtacacgatc accgcggacc tgctcgcctg gggggcgggc agggcggcgg aacacggtgt
     2401 ccggggcagc ggagccctgg acccggtggc ggccttcggt ctcgacgagc tgtcggcagg
     2461 cgccgcggca gcggggatcg tcccggccga cgggtcctag caagcgccag cgagctcaca
     2521 gtcggtcgcg gcacccctgg cgtcgttccg tcaccggccg atcacgtggt gacggcggat
     2581 cgacggtgag ctgccgtaga ggcggtcggc gagcgtgcat gcaccggcgg cggctactct
     2641 cacgcgccgt gacgaccgat cagcgccggc cgctggccga cccctcgctg gtcggcctca
     2701 tccggatgcc ggactcctcg gcgagccctc tggtatcgct gctgcgcagg ctcgcgatcg
     2761 cgttcggcgc gctggccgcc gccgccctgg tcgtctactt cggtggcaac ggctacgtcg
     2821 acaacaacag caacacaccg atctcgttgg gtgatgcgtt ctactacgcc accgtgtcgc
     2881 tgtcgacgac cggctacggg gacatcgtcc ccgtcacgcc ggaggcgcgc ctcctcacta
     2941 cggtggtcat cacgccgctg cggttgctgt tcctcatcgt tctggtcggg accacggtcg
     3001 agctgctcac cgagcgttcc cggcagggag tccgcatcga gcgctggagg tcccgggtgc
     3061 gcaaccacac catcgtcgtc ggatacggca cgaaaggccg tgccgccgtc gagacgctcc
     3121 tgggggacgg ggccgcgccc accgacatcg tcgtcgtcga caccgaccgc acccagctcg
     3181 acgccgcgtc cgcgctcggg ctggtgacgg ttacgggcaa cgcgaccagg tccagtgtcc
     3241 tgcggatcgc cggcctgcag caggccgccg cgatcgtggt ggccaccaac cgcgacgaca
     3301 cggcggtgct ggtcactctc acggcccgcg agctcgcgcc ggacgtgcgg atcgtggcgt
     3361 cggtacgcga gtcggagaac gtgcacctcc tgcggcagtc cggcgccaac tcggtgatcg
     3421 tctcggcgga gacggcgggc cgcctgctgg gcatggcgac caccaccccc agcgtggtcg
     3481 aggtggtcga ggacctgctc acccccgacg cgggattcgc gatcagcgag cggccggtgg
     3541 aggactcgga ggtgggcggg tcgccgcgcc acctcgccga catcgttctc ggcgtcgtgc
     3601 gcggtgacga gctcttccgc gtggacgcct cggccgtcga cgccctcgag cgcggtgacc
     3661 ggctcctcta cgtgcgcaag gcgagcctgc ccgacgagga ctgatgccac cgtgtcgacc
     3721 gtcaaggcga cgatgtccag cgcactcacg aggctcgacc tggcgaaccg gatccagctg
     3781 gccatcgtcg cccacgagtc acgtcaaggc ccgccctcgg cgggatgaca gggctccgtc
     3841 cgcgtctccg tctcaggaga tcggcaggat cagcgcaaag gcagcgcccg taccgtcagg
     3901 ggggtcgacg cagcgcaggt cgccgccgtg cgcccgggcg atgccccgtg cgatcgcgag
     3961 tccgagcccg gcgccgccgc cgtcggccgt gcgggcctcg tccagctgca cgaggcggtc
     4021 gaagatccgt tcccggtcgg cgggcggcac ccctggcccg gtgtccgtca ccagcagcgt
     4081 cgccgctccg cgcaccgaca cgccgaccgt gatccggcca ccgctcggcg tgtagcgccg
     4141 tgcgttgtcg agcaggttgc ccagcacctg gccgagccgt gccgggtcgc ccgggacgac
     4201 ggcggagtcg ccgcggacct cgaccgtccg gttcggcgcg agcaaccgca cccgctccgc
     4261 ctcggcacgg gcgagctcca ggaggtccac cggctcatga cgcagctcga tgcccgcgtc
     4321 gatccgcgcg agcgcgagaa ggtcgtccac caaccggccc gcccggtggc tctcccgcaa
     4381 cagcaacagg tgcagccggt cgcgctcctc ggcgtccagc cccggggcga tggcggcctc
     4441 tgccgcggcc tgcaccccgg ccagcggggt gcgcagctcg tgcgcggcat cggcgacgaa
     4501 ctgcctggtc cgcgcctccg acgcccgggc cgacgcctcg gcgccctcca gctcgtcgag
     4561 catcgcgtcg aacgccgacg cggtacgacc gagctccgtg tcggtccggg acggtgcgag
     4621 cctgcggccg cggtcgccgc gggtgatcga gcgggccagc gcagtcatgg cgtcgagcgg
     4681 ggcgagcgcg acgcgagtgg tgagcagcgt gaccaccccg gcaacggcca gcgccgcgat
     4741 gctgagcagc aacagcgtgc gccgtagcaa ccgctgtgca gcggtcacct gggcgccgtc
     4801 ggcaacgagg gtgagccggg cgccattgac cagtggctgc tgtaacacct ggtcgctccg
     4861 ccccgggccc gcacccgagc cgtcacccca gccatcccgg cctgtgtagt ccccaggacc
     4921 ctggcgccac ggtggcccgg gcgcgcgttc gggctcgccg ccgtcgtaca ccgcgccgtc
     4981 ggctgcgacg acgaccgcgc ggatccccgg cgcctccacc cgccgcacca cttccggggt
     5041 cggcagcccc tctgtcacga gcctgtcggc cagcgccgcc cggacggtga gctcgtcatg
     5101 caggtgggcg cgggtctggg tagcgaacac gacgtcggtc agtactccga cgagcaccag
     5161 aaccgcggcc agcacgagca tgctcagcgc cgtgacccgg cggcgcaggg acacggtccg
     5221 cagccggcct gcgctcctca cgcaggagtc tccggaccgc cgtcgccgcc ggcgcgcagc
     5281 acgtacccca gcccgcgcac ggtgtgcacc agcctcggct cgccgagctt gcgccgcagc
     5341 gccgagacat gcacctccac caggttgggg tcgtagtcgg tgtagcccca cacgcgcgtg
     5401 aggatctgcc ccttgctgac
//
LOCUS       DASRSC010000004         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2130936,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000004 DASRSC010000000
VERSION     DASRSC010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2130936"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFE39_00125"
     CDS             complement(<1..713)
                     /locus_tag="VFE39_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET6257034.1"
                     /translation="MSGTEGPRVGSSNDTGPISEGDTAAMAELGLHPGEFVSADDDQD
                     VDAGEQGEPGALPPAPVLAVVGRPNVGKSTLVNRLIGRREAVVQDIPGVTRDRIAYDA
                     LWNGRRFTLVDTGGWEPDATGLQAAVAAQADMAIRTADAVLLVVDASVGATTTDEAVA
                     RVLRRSDRPVLLAATKVDDDRLTSDTAALWRLGLGEPHPVSGLHGRGSGDLLDAVLAA
                     LPESPRDLTGGGAAGPRRVA"
     gene            complement(710..1351)
                     /locus_tag="VFE39_00130"
     CDS             complement(710..1351)
                     /locus_tag="VFE39_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6257035.1"
                     /translation="MNADLPPGSWPWLHELARWVGAWLFRPFYRMRVHHRARIPATGP
                     VVLVANHSAFVDGPLLFGLLGRRAVFLTKQEMFVGPIGWWLPRIGQLAVRRGEPDRRP
                     LLVAVDVLRAGGLIGVFPEGTRGSGEVAAAQHGAAWLARTTGAVVVPVVCRGTRRPDG
                     TGRRWRPRVDVLVGEPLTIPSGGGRAGLITATETVRAALAGMVRELDDVRSRT"
     gene            complement(1348..2019)
                     /gene="cmk"
                     /locus_tag="VFE39_00135"
     CDS             complement(1348..2019)
                     /gene="cmk"
                     /locus_tag="VFE39_00135"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET6257036.1"
                     /translation="MDGPSGTGKSTVARRLAASCDAAYLDTGAMYRALTLAVLRAGLD
                     GSDPAAEGVALGAVADLRSVVDPTAPRILLLGEDVEAEIRGPEVTAAVSAVSAVPAVR
                     AALVERQRVIVADAVAAGGIVAEGRDIGSVVVPDAPLKVYLTASEHVRAARRGAQDRK
                     AGRNGDVGTVLADVRRRDRLDSTRRTSPLHAAADAVVLDTDDLSVDAVLAELLRLVRE
                     RGLLL"
     gene            complement(2198..2545)
                     /gene="aroH"
                     /locus_tag="VFE39_00140"
     CDS             complement(2198..2545)
                     /gene="aroH"
                     /locus_tag="VFE39_00140"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET6257037.1"
                     /translation="MRGAIQVDGDTRQEILDGSAELVRNVLDRNGLSADDIISIVFTA
                     TPDLTAEFPAYAARLLGLTDVPLLCASEIAVPGALPRTLRLLAHVETERSRAELRHVY
                     LRGAAALRTDLPQ"
     gene            complement(2563..>2734)
                     /locus_tag="VFE39_00145"
     CDS             complement(2563..>2734)
                     /locus_tag="VFE39_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET6257038.1"
                     /translation="EGRKHVVRRLHDEVGHPVQRLVRTAIGDVRLGDQRPGRIRPLTR
                     AEVGSLYRSVGM"
BASE COUNT          391 a         1058 c          943 g          342 t
ORIGIN      
        1 gcgacacggc gcgggccggc ggctccgccg cccgtgaggt cgcgcgggct ctccggcagc
       61 gcggcgagca cggcgtcgag caggtcgccg gaaccgcgcc cgtgcagtcc gctcaccggg
      121 tgcggttcgc cgagcccgag tcgccacagg gcggcggtgt cggacgtcag ccggtcgtcg
      181 tcgaccttcg tggcggcgag caggaccggg cggtccgagc ggcgaagcac cctggccacg
      241 gcctcgtcgg tggtggtggc gccgacgctc gcgtcgacca ccagcagcac cgcgtcggcc
      301 gtccgtatcg ccatgtccgc ctgagcggcg acggcagcct gcaacccggt ggcatcgggc
      361 tcccagcccc cggtgtcgac cagggtgaaa cggcgcccat tccacagggc gtcgtaggcg
      421 atccggtcgc gggtgacacc cgggatgtcc tgcacgaccg cctcgcgccg cccgatcagc
      481 cggttgacca gagtcgactt gccgacgttc ggtcgcccga cgacggcgag gaccggcgcg
      541 gggggcagcg cacctggctc gccctgctca ccggcgtcca cgtcctggtc atcgtcggcg
      601 ctcacgaact cgccggggtg gagccccagc tcggccatcg cggccgtgtc cccttcgctg
      661 atcggcccgg tgtcattgct cgaccccaca cgcgggcctt cggtaccact cacgtcctgc
      721 tccgaacatc atcgagttct cggaccatcc cggccagtgc ggcccggacg gtctcggtgg
      781 cggtgatcag gccggcccgg ccaccgccgc tggggatggt cagcggctct cccacgagca
      841 cgtccacccg cggtcgccag cgcctaccgg tcccgtcggg tcgacgggta ccacggcaga
      901 ccaccggaac aaccaccgca ccggtggtgc gcgccagcca tgccgccccg tgttgggcag
      961 cggccacctc gccgctcccg cgggtgccct ccgggaacac cccgatcaga ccacctgcgc
     1021 ggagcacatc caccgcgacc agcaacggcc gccggtcggg ctccccgcgg cgcaccgcca
     1081 gctgcccgat ccgcggcaac caccagccga tcggacccac gaacatctcc tgcttcgtga
     1141 ggaacaccgc gcgccgtccc aatagtccga acagcagcgg accgtcgacg aacgcgctgt
     1201 ggttggcgac gagcacgaca gggcccgtgg ccgggatgcg agcgcggtga tgcacccgca
     1261 tccggtagaa cgggcggaac agccacgcac cgacccagcg ggccagctcg tgcagccacg
     1321 gccacgaacc cggtggcagg tccgcgttca caacagcagt ccccgctccc gcaccagtcg
     1381 caacagctcg gccagcacgg cgtcaacgct gaggtcgtcg gtgtcgagca cgaccgcgtc
     1441 ggcggcggcg tgcagcggtg aggtcctgcg tgtggagtcg agacggtcac ggcgccggac
     1501 gtcggcaagc acagtaccga cgtccccgtt tcggcccgcc ttgcggtcct gggcgccgcg
     1561 gcgggcggcc cggacgtgtt ccgacgccgt gaggtacacc ttgagcgggg cgtccggcac
     1621 cacgacgctg ccgatgtcgc ggccctccgc cacgatcccg cccgctgcca ccgcgtccgc
     1681 gacgatcacg cgctgccgct ccacgagcgc cgcgcgcact gcgggtactg cggagactgc
     1741 gctcaccgcg gccgtgacct ccgggccgcg gatctcggcc tcgacgtcct cgcccagcaa
     1801 caggatccgc ggtgcggtgg ggtcgactac cgatcgcaga tcagcgacgg caccgagcgc
     1861 gacgccttcg gcggcagggt ccgagccgtc gaggcccgcg cgcaacaccg cgagggtgag
     1921 tgcgcggtac atggcaccag tatcgaggta ggcggcgtca caggaggcag ccagccgccg
     1981 cgcgaccgtg gacttcccgg tgcccgacgg gccgtccatc gccacgaccc cgtgcaaccg
     2041 ccctgccacc gttcgcccct gtccgggcca gcggcccacc acctgccgcg cgccgaccgc
     2101 ggacgccgac catcatgccg tgcggcccaa cggtgatctc agctcggggc accgaccggc
     2161 tcggtggggc cccaggccgg ctgctgtggc cgtccgacta ttgcggcaga tcggtgcgca
     2221 gcgccgccgc accccgcaga tacacgtggc gcagctccgc gcgggaccgc tcggtctcga
     2281 cgtgcgccag cagccggagc gtgcgcggca gcgcccccgg gacggcgatc tccgacgcac
     2341 agagcagcgg tacgtccgtc agcccgagca ggcgtgcggc gtaggcggga aactcggcgg
     2401 tgagatccgg ggtcgccgtg aacacgatcg agatgatgtc gtcggcggag agcccgttcc
     2461 ggtcgagcac gttcctgacg agctcggccg aaccgtccag gatctcctga cgggtgtcgc
     2521 cgtccacctg gatcgcgccc cgcaccgccc gcaccgccac tgctacatac cgaccgaccg
     2581 gtagagcgaa cccacctcgg cccgggtcag gggacggatc cggcccggtc gttggtcacc
     2641 gagccgtacg tcgccgatcg cggtgcgcac caggcgctgc acggggtgcc cgacctcgtc
     2701 gtgcaacctg cgcaccacgt gcttgcgccc ctcg
//
LOCUS       DASRSC010000005        12557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1639613,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000005 DASRSC010000000
VERSION     DASRSC010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12557
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1639613"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1524
                     /locus_tag="VFE39_00150"
     CDS             <1..1524
                     /locus_tag="VFE39_00150"
                     /inference="COORDINATES: protein motif:HMM:NF026246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HET6257039.1"
                     /translation="LVALVWFAVIAVAARAREVAVPTPLWLAGSTTALAFAALWLIPS
                     KWTNLFGALAGVGPAFLALLLVMAVPLVRQVLQGAKLPIGALVAAAASFVVAIALGWH
                     GPNQWAYAWLEGVHRPELPPAVKNIALDRPLLWALVVVVAGLVGGAGARRARLIGRGD
                     VRLGALRAVPVVVLISLVGTTVYTVGTFGAAAAQAAPRESIWAQGLADPTGAQCGAAG
                     VVRVLDPSTASTLGIARGLPAPAAPIGFVPGAGFYEGDRPQGPAGDEVWGSLDFRNGR
                     GANGEMTTGWYTLPSVLVDGAATTVLAAGTLTDGNRLTAIYGRSSGASVAPVGEEPLT
                     DTDTEPSWRTFTLQPPAGADVVRLEAVDTTSMVDGWLAFSAPAVQRPVVLSEYLPAAA
                     PVALAWPLAFAYPCQRQPTMVDGITEAPEYAVLWGDGPLSGFADGTWVPFRGGAFAHV
                     RRTQSVQQLSVLRGVDPHIQVYAFRTPLARRAYTVTETTRTVFGASTGTGTGPATQR"
     gene            1567..4596
                     /locus_tag="VFE39_00155"
     CDS             1567..4596
                     /locus_tag="VFE39_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052636 - arabinosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071766 - Actinobacterium-type cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase domain-containing protein"
                     /protein_id="HET6257040.1"
                     /translation="MLSPARSAPPPEPDSPREPHRSGRDSHGDHPSTPTARGPRRAVL
                     VLGLLAALAAIAFPFAPVHQPEVRYTWTASDGSAAIPLMPYQPVDLTVTTSCAAIRGT
                     GVPGTARPDRVVLSTVPLRPDPTADRLHGLRLTATGDAVRVASAGVELGTVPLPSGPC
                     SFSLVSDPEHTAVLVNGVSALTHDSDVRPDVAGAFSDVPTGVDMALTADTRFQTTITP
                     LKTALGVVCVLALLGMLVALRRADRAVTAPVRLLPRRWWVPRPVDAAVVALFGVWWIV
                     GGITVDDGYIAGIVRSIGSNGFIGNVYRWLNAPEAPFSWFYDVYYAWSLVSPSTLWMR
                     LPSTLLGLLCWWLLSRLVLPRLGRFAARRSVPWLAALGLATWWVPLNLGLRPEPWVAV
                     GGLVVFLAVERAVAVRTVTPLAVGLVVAGATTAVTPAGLMAFTPFLAAALPVLRLLRA
                     RRDLHVLPLLAALVAAPASAVLLMVPDQSLAGMLEATRVRTLIGGGMEWYQEYERYST
                     LLDAASFEGSIGRRAAVLLTLLAAVGVLWRLHRARTGIATGPARRLVLGFLLSLATLT
                     ASPTKWTQHFGDVAGYGAAVLVLGMVAWSAGGSTSTVRERPGKLMAGLGAVTVVGALV
                     LAGYNTWPYASNWFTPSFSTLPPQLGNVPLATVAIAVGGLLLFGLLARSAWLRAGGAA
                     EVPVPSWVPAPAPLVAVLMVAVLGLQVLSFVRVAAEHRGSYTLASDSIATMRGEPCGL
                     QRLLSVETDPAAGLLPIASGPPTPRTLPVDVGGRTLPGVAVTGRMTTSWFRLDPEQRS
                     DTLPVVVTTSGETQPGDALFIEFGAGERVLERRLVAVNDPGPRDDRQLAPAGATTVRL
                     AVDAPNPGPRSPAVASLPRVPRLTPMTDVLPVGTSAILDWPVAFLFPCVVPEPLPLGT
                     AGLAQWRVGPPAGDPAAGITYAAGFGGPFAASRLLVTEQRMATYLTGDPTRDAAELYR
                     WIPLEPLTRPQPVVTDRTVSGWHRDGHARVPGLDRVS"
     gene            4700..6601
                     /locus_tag="VFE39_00160"
     CDS             4700..6601
                     /locus_tag="VFE39_00160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6257041.1"
                     /translation="MATEPPADAAGNSVPEPSEGATRPRLVVQRGLFFGPSALVPEDL
                     YSIVEKGSASRERAQVALEPNTRVNTNTYFGRFHATYWQRWTDVGEIEVDAVVTGTGR
                     VRLIASDTNKVWRIIEAQDVRDAEGSRIRLTGPVDRFVDGGGMWLELTTETGHLTVAD
                     VRWAVSDARPGPRTDVVICTYNRVDDCLNTLQAMVADPEALSCIGTVQVVDQGSDPLE
                     SRPRFAEVAAGLGERLRYVRQPNLGGAGGFTRGLFDATAGEPDDHADVLLMDDDVLLD
                     PEILIRLTGFAASTRHPMIVGGQMLNLLHPAHLHISAEYADPELLQVGMPVPGALKEA
                     YLIGLDERQLPINQERRVDTEYNGWWSCLIPAAIVRAIGYPLPLFFQWDDIEYGYRAR
                     AHGFPTVALPGAGVWHADFGWKDWDEWHRYFNMRNGLITAALHTGFSVRRIAGRLGQL
                     LSQYLISMQYGLSATLLQAMEDFLEGPEILRDGSAGAAAEIRRIRARYAETAMHPVSD
                     IVDDFRELQVVRAANPPGHERLTWLKRAAYLLTNRSVHRSGFVPAGDAHWWHVSTFEH
                     AVVTDMSEQGIRVRTRDKAVALQLAKRAARTFRRFLAEGPTAARRYRNEMGHLTSRAN
                     WARLYGLDD"
     gene            complement(6656..8653)
                     /locus_tag="VFE39_00165"
     CDS             complement(6656..8653)
                     /locus_tag="VFE39_00165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6257042.1"
                     /translation="MTQTVVTAEETATPAGEAAASSLLQRVILPRSGDPMSVRSLYLD
                     ERTGLRMTTVPEPTGAPPGPKVDLTGTAMGVRRLRVTSRISAVVPEQTEVSFGAYFNA
                     FAAGYWRRWSRLTELHLRLVVEGTGRVDVYRTKSDGSQIFVRGIVITGPGLQELDLDL
                     DLRPFEDGGWYWFDLTTDSSELTLHSGGWYAAEAATGRAAVTIGMPTFNRPADCVATL
                     TAIGEDPIVLDAVTAVILPDQGTKKVRDEPGFEAAAAVLGDKLRIIDQPNLGGSGGYA
                     RVMYEALGSTDCEQILFMDDDILLEPDSVLRAVAFSRFSREPMLVGGQMLSLQARSQL
                     STMGEVVDRNTFLWRNAPGTEPHHDLADRTLRQTPWLHRRTDVDYNAWWMCLIPRAVA
                     EDLGLPLPLFIKWDDAEYGLRARSRGYRTATVPGIAIWHMSFLEKDDTSDWQAYFHYR
                     NRFVAAALHGPDNPKALLRDTIKRTLRHLLLMEYSAVALQAMALRDFLAGPEALFPKL
                     ATVLGEVRAERAKYDDGRPLDSATQVPLSELDALAAQVFPPPPTRKIPIAKGLARSVL
                     HNLRAPNPDHQAVPQRNIPSRQAQWFVLSQLDSATVSTPDGRGVTFRRRNPELFRSMI
                     KNSVAQHREIAKAWPELRRRYRAALPELTSHEAWKREVFDR"
     gene            complement(8679..9875)
                     /gene="glf"
                     /locus_tag="VFE39_00170"
     CDS             complement(8679..9875)
                     /gene="glf"
                     /locus_tag="VFE39_00170"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HET6257043.1"
                     /translation="MSYPGYDLVIVGSGFFGLTVAERSASELDKRVLVLERRSHIGGN
                     AYSEPEPETGIEIHRYGAHLFHTSNERVWEYVNRFTDFTDYQHRVFARVGEQVYAFPM
                     NLALINQFFGRSHTPDEARALIAEQAGEIDTKDAKNLEEKAVSLVGRPLYEAFVKGYT
                     AKQWQTDPTKLDPSIIARLPVRYTFNNRYFNDTYEGLPVDGYTAWLEKMADHPNVEVR
                     LDVDYFDVRDQIPTGTPTVYTGALDRYFDYAEGELGWRTLDFEQEVRHDTGDFQGTPV
                     MNYNDEDVPFTRIHEFRHFHPERAYPTDKTVIVREYSRFAESGDEPYYPINTPDDRVK
                     LQRYRDLARKETANANVLFGGRLGTYKYLDMHMAIGSALSMFDNRIAPYFTDGRALAG
                     TESSED"
     gene            10030..12006
                     /locus_tag="VFE39_00175"
     CDS             10030..12006
                     /locus_tag="VFE39_00175"
                     /inference="COORDINATES: protein motif:HMM:NF042625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6541 family protein"
                     /protein_id="HET6257044.1"
                     /translation="MEAGAAGAGIHVVALYVVVLWVPGLLLGWLAGMRGWTLAAGAPL
                     LTYTVAGLAGPAFSAARISWTPASAGVLLAILTAGAAALRWSARRRNGATEDDVEAHW
                     AGRVHLAVAAALTAVVVFGAVVIWTGIGRLSAVPQDWDAAFHANGIRWIADTGDSSLV
                     GMGSVNWYEDGVHVYYPNAYHLVAATVLRLTSAGIPTVLNAQTVLLPGICALVVVALV
                     HRLRGRAVLAVAAAACSVSITSFYDMLWRGPLLPFVTGAALMPLVAVLVLDLLDAGTG
                     RSRMAPGLLFASGLVGLICLNPATLFSAVLFVVPALGQRWASRPSLLRREPLVILGAG
                     VVAAVLALPQLLASLGSAEGAPFDWPATLSQLEAVGQLLTLSHGGLPPQWCLVVAAAV
                     GLARYGNLSQLRWIGVAGAAFGALFVLSASSDAFWVNAITRPWWNDRWRLIGLCVVPI
                     AVVAGHGLAELQRSTAAALGAAARMSRGDSKVTAGVSRAAPIVAVGIVGALFVGASND
                     LYVDRNVARMRLSAPDGPVVSSLEVDAMHALAAVVPAGQRVLNDRGDGSAWMYAIAGV
                     LPVAGHYNASRIGPDAQLLADRFNRYPDDPAVQAAIERLNISYVMLDRGFVRSSWSRA
                     PGLVGLDGASWLDKVYENPDAVIYRIRSGIPSRP"
     gene            12045..>12557
                     /locus_tag="VFE39_00180"
     CDS             12045..>12557
                     /locus_tag="VFE39_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257045.1"
                     /translation="MSGNWSVGTFADEPGARPLEHVSAEPSPTSRPASTGGRAGLRRL
                     AVRAGEAAAALGAAVVAVQLSRSIMVDPLDRVGQVSGVAGLDLRFVVLGLLVLAACAA
                     ATRRPAAWAIVSRFACAAVAGLATGLVAGGILLALRGTQWPLYANWGDSGQLIRWADD
                     LLAGRPVPADY"
BASE COUNT         1623 a         4480 c         4454 g         2000 t
ORIGIN      
        1 ctggtcgcgc tcgtctggtt cgcggtgatc gcggtcgcag cacgcgcccg tgaggtcgcg
       61 gtgccgacac cgttgtggct ggccggctcg accaccgcgc ttgcgttcgc cgcgctctgg
      121 ctcatcccgt ccaaatggac caacctcttc ggggcgctcg ccggtgtcgg cccggcgttc
      181 ctggcgctgc tgctggtgat ggcggtgccg ctcgtgcggc aggtgctgca gggcgcgaag
      241 ctgccgatcg gcgcgctcgt cgccgccgcg gcctcgttcg tcgtggcgat cgcgctcgga
      301 tggcacgggc cgaaccagtg ggcctatgcg tggctcgagg gggtccacag gccggaactg
      361 ccgcctgcgg tgaagaacat cgcgctggac cgaccactcc tctgggcgct ggtcgtggtc
      421 gtcgccgggc tggtcggcgg cgccggcgcg cgacgcgcgc gcctgatcgg gaggggggac
      481 gtccggctgg gtgcgctgcg cgcggtgccc gtcgtcgtgc tgatctcgct cgtgggcacc
      541 accgtctaca ccgtcggcac gttcggagcg gccgccgccc aggccgcacc gcgcgagtcg
      601 atctgggcgc agggcctcgc ggacccgacg ggcgcgcagt gcggtgcggc cggggtggtg
      661 cgggtgctcg acccctccac cgcgtcgacg ctggggatcg cgcgaggtct gcccgcaccg
      721 gctgcgccca tcggcttcgt acccggcgcc ggcttctacg agggcgaccg tccgcaggga
      781 ccggcggggg acgaggtctg gggcagcctc gatttccgca acggccgcgg cgccaacggc
      841 gagatgacca ccggctggta cacgctgccg tccgtgctgg tggacggtgc tgccaccacc
      901 gtgctggccg cgggcacgct cacggacggc aaccgcctca ccgcgatcta cggccgcagc
      961 tccggggcga gtgtcgctcc cgtcggcgaa gagccgctca ccgacaccga caccgaaccc
     1021 tcgtggcgca ccttcaccct gcagccgccc gccggtgccg acgtcgtgcg gctcgaggcc
     1081 gtcgatacca cgagcatggt cgacggctgg ttggccttca gcgcacccgc cgtccagcgg
     1141 cccgtggtgc tgtccgagta cctgccggcc gccgcaccgg tggcactggc gtggccgctc
     1201 gcgttcgcct atccctgcca gcgccagccg accatggtcg acggcatcac cgaggcaccg
     1261 gagtacgccg tgctgtgggg cgatgggcca ctgtccggct tcgccgacgg cacctgggtg
     1321 cccttccgtg gcggcgcgtt cgcccatgtg cgtcgcaccc agtcagtgca gcagctctcc
     1381 gtgctccgcg gggtcgaccc gcacatccag gtgtatgcgt tccgcacccc gctggcccga
     1441 agggcctaca cggtgacaga gacgacccgc acggttttcg gagcgtcgac cggcaccggc
     1501 acaggcccgg ctacgcagcg gtaaccccca ccctggcgtg gtgtggtgtg gacctaaggt
     1561 gacggagtgc tctctcccgc acggtcggcc cccccgcccg agccggacag ccctcgggaa
     1621 ccgcaccgca gcggtcgcga cagtcacggc gaccatccgt ccactccgac cgcgcgcggc
     1681 ccccgccggg ccgtcctcgt gctcgggctg ctcgccgcgc tcgccgctat cgcgttcccg
     1741 ttcgccccgg tgcaccaacc ggaggtccgt tacacctgga cggcctccga cgggtcggcg
     1801 gcgatcccgc tcatgcccta ccagcccgtc gacctcacgg tgacgacgag ctgcgcggcg
     1861 atccgcggca ccggggtccc gggcacggcg cgcccggacc gcgtggtgct ctccaccgtc
     1921 ccgctgcggc ccgatcccac ggccgaccgg ctgcacggcc tgcgcctcac ggccacgggc
     1981 gacgcggtgc gcgtcgcgag cgcgggcgtg gagctcggca cggtgccgct gccctcgggt
     2041 ccgtgcagct tctcgctcgt ctccgacccg gagcacacgg cggtactggt caacggcgtt
     2101 tctgccctca cccacgacag cgacgtgcga cccgacgtgg ccggggcctt cagcgacgtg
     2161 cccacgggcg tcgacatggc cctcaccgcc gacacccgct tccagaccac catcaccccg
     2221 ctcaagaccg cgctgggcgt ggtgtgcgtg ctcgccctcc tcgggatgct cgtggcgctg
     2281 cgccgggccg accgcgcggt caccgcgccc gtgcggttgc tcccccgccg ctggtgggtc
     2341 ccgcgtcccg tcgacgccgc ggtggtcgcg ctgttcgggg tctggtggat cgtcggcggg
     2401 atcaccgtgg acgacggcta catcgccggc atcgtgcgca gcatcgggag caacgggttc
     2461 atcggcaacg tctaccgctg gctcaacgcg cccgaggccc cgttcagctg gttctacgac
     2521 gtctactacg cctggtcgct cgtctcgccc tcgacgctgt ggatgcgact gccgtcgacg
     2581 ctgctcgggc tgctgtgctg gtggctgctc tcccggctcg tactgccccg gctgggccgg
     2641 ttcgccgcgc ggcggtcggt gccgtggctg gccgcactcg gcctggccac ctggtgggtg
     2701 ccgctcaacc tcgggttgcg tccggagccg tgggtggcgg tcggcgggct cgtggttttc
     2761 ctcgccgtcg agcgcgcggt ggccgtccgc acggtcacac cgcttgccgt cgggctcgtg
     2821 gtcgccggcg cgaccaccgc ggtgaccccg gccggactga tggccttcac cccgttcctc
     2881 gccgccgcac tgcccgtgct gcggctgctg cgcgcccgcc gcgacctgca cgtactgccg
     2941 ctgctcgccg cgctggtggc cgccccggcc tccgcggtcc tgctgatggt gcccgatcag
     3001 agcctcgccg gaatgctcga ggccaccagg gtgcgcaccc tgatcggcgg cgggatggag
     3061 tggtaccagg agtacgagcg gtactccacg ctgctggacg cggcatcgtt cgagggttcg
     3121 atcggacgcc gggccgcggt gctcctcacg ctgctcgcgg ccgtcggcgt gctgtggcgg
     3181 ctgcaccgcg cgcgcaccgg gatcgccacc ggccccgccc ggcggctggt gctcggtttc
     3241 ctgctctcgc tggcgacact gaccgccagc cccacgaagt ggacgcaaca cttcggcgac
     3301 gtcgccggct acggcgccgc cgtactggtg ctcggcatgg tggcctggtc ggcaggcggg
     3361 agcacatcga ccgtgcgtga gcggcccggc aagctgatgg ccgggctcgg cgcggtgacg
     3421 gtcgtcgggg ctctcgtgct ggccggctac aacacctggc cgtacgcgag caactggttc
     3481 acgccaagct tcagcacact cccgccccag ctggggaacg tgccgctggc caccgtcgcc
     3541 atcgccgtcg gcggcctcct cctgttcggg ctgctcgccc ggtcagcctg gctacgcgcc
     3601 ggcggggccg ctgaggtccc cgtgcccagc tgggtgcccg ctccggcgcc gctcgtggct
     3661 gtcctcatgg tggccgtgct ggggctgcag gtgctgagct tcgtgcgcgt cgccgccgag
     3721 caccgaggca gctacacgct cgcctccgac agcatcgcca cgatgcgggg cgagccgtgc
     3781 gggctgcagc ggctgctgtc cgtcgagacc gatccggcag cgggtctgct gccgatcgcg
     3841 tccggcccgc cgaccccccg cacgctgccg gtcgacgtcg gtggtcggac actgcccggc
     3901 gtggcggtca ctggtcggat gaccacgtcg tggttccggc tcgacccgga gcagcgctcg
     3961 gacaccctcc ccgtggtcgt cacaacctcc ggtgagaccc agccgggtga tgcgctgttc
     4021 atcgagttcg gtgcgggcga gcgggtgctc gaacgccgcc tcgtcgccgt caacgacccc
     4081 gggccgcgcg acgaccgcca gctcgccccg gccggtgcca ccacggtgcg gctcgcggtc
     4141 gacgcgccca acccgggccc gcggtctccc gccgtcgcgt cgctcccgcg ggtgccccgg
     4201 ctgaccccga tgaccgacgt gctgccggtg ggtaccagcg cgatcctcga ctggcccgta
     4261 gcgttcctct tcccctgcgt cgtccccgaa ccgctgccgc tgggcaccgc cgggctggcg
     4321 cagtggcggg tggggccgcc ggcgggcgac cccgcggccg gcatcaccta cgcggccggc
     4381 ttcggcgggc ctttcgccgc atcgcggctg ctggtcaccg agcagcgaat ggcgacgtac
     4441 ctgaccggcg accccacacg cgacgcggcg gagctctacc gctggatacc cctcgaaccg
     4501 ctcacccgtc cgcagcccgt ggtcacggat cgcaccgtct ccgggtggca tcgggacggg
     4561 cacgcccgcg tcccgggact cgatcgggtg agctgacccc atcggtgcac aacggtctgc
     4621 ttaccccaca atggacatcc aacccagcac ccgacagagg acggccgtct gcatgcttcg
     4681 cataacagaa agcacggaca tggcaacgga gcctccggcc gacgctgcgg ggaacagcgt
     4741 gccggagccg tcggaggggg ccacccggcc gcgcctcgtc gtgcagcgcg ggctgttctt
     4801 cggaccgtcg gcgctggtac cggaggacct gtactccatc gtcgagaagg gcagcgccag
     4861 ccgcgaacga gcgcaggtgg cgctggaacc gaatacccgc gtcaacacca acacctactt
     4921 cggccggttc cacgccacct actggcagcg ctggacggac gtcggggaga tcgaggtcga
     4981 cgccgtcgtc accggcaccg gccgggtgcg cctgatcgcg tcagacacca acaaggtgtg
     5041 gcgcatcatc gaggcccagg acgtccggga cgccgagggc agcaggatcc gcctgacggg
     5101 gcctgtcgac cgcttcgtcg acggcggcgg catgtggctc gagctcacca ccgagacagg
     5161 ccacctgacc gtcgccgacg tgcggtgggc cgtctccgat gcccgtcccg gtccgcgcac
     5221 cgacgtcgtg atctgcacgt acaaccgggt cgacgactgc ctcaacacgc tgcaggcgat
     5281 ggtcgccgat ccggaggcac tgagctgcat cggcacggtg caggtcgtcg accagggcag
     5341 cgaccccctg gagtcgcggc cgcgcttcgc ggaggtcgcg gccgggctcg gtgagcggct
     5401 gcgctacgtc cgccagccca acctgggcgg tgctggaggg ttcacccgcg ggctgttcga
     5461 cgccacggcg ggcgagcccg acgaccacgc cgacgtcctg ctcatggacg acgacgtgct
     5521 gctcgacccc gagatcctca tccggctcac cggcttcgcc gcgagcaccc gccacccgat
     5581 gatcgtcggc gggcagatgc tcaacctgct gcaccccgcg cacctgcaca tctccgccga
     5641 gtacgccgac cccgaactgc tccaggtcgg catgcccgtg cccggcgcgc tgaaggaggc
     5701 ctacctgatc gggctcgacg agcggcagct gccgatcaac caggagcgtc gcgtcgacac
     5761 cgagtacaac ggctggtggt cctgcctgat cccggccgca atcgtgcggg cgatcggcta
     5821 cccgcttccg ctgttcttcc agtgggacga catcgagtac ggataccggg cgcgtgcaca
     5881 cggcttcccg accgtcgccc tgcccggagc cggtgtgtgg cacgccgact tcggctggaa
     5941 ggactgggac gagtggcacc ggtacttcaa catgcgcaac ggcctgatca ccgcggcact
     6001 gcacaccggc ttctccgtgc ggcggatcgc cgggcggctc gggcagttgc tgtcgcagta
     6061 cctgatcagc atgcagtacg ggctgtcagc caccttgttg caggccatgg aggacttcct
     6121 cgagggcccg gagatccttc gcgacggctc ggccggtgcc gccgccgaga tccggcggat
     6181 ccgggcccgg tacgcggaga ccgccatgca ccccgtgtcc gacatcgttg acgacttccg
     6241 cgagttgcag gtcgtgcggg ccgccaaccc ccccggccac gagcggctca cgtggctcaa
     6301 gcgcgccgcg tatctgctca ccaaccgatc ggtgcaccgc tccggattcg ttcccgcagg
     6361 cgacgcgcac tggtggcacg tctcgacctt cgagcacgcc gtggtcacgg acatgtcgga
     6421 gcagggcatc cgcgtccgta cgcgggacaa ggcggtggcc ctgcaactcg cgaagcgcgc
     6481 cgcccggacg ttccgccggt tcctggcaga gggcccgacc gcggcccggc gctaccgcaa
     6541 cgagatgggc cacctcacca gccgggccaa ctgggcccgg ctgtacggcc tcgacgactg
     6601 agcgggttga ccgcactgcg gtccggacgg ctggttgcgg tccggaccgc ggcggtcaac
     6661 ggtcgaagac ctcacgcttc caggcctcgt ggctcgtgag ttcagggagt gccgcccggt
     6721 agcgccggcg cagctccggc catgccttgg cgatctcgcg gtgctgcgcc accgagttct
     6781 tgatcatgga gcggaacagc tccggattcc ggcgacggaa ggtcactccg cgcccgtcgg
     6841 gcgtggagac cgtggccgag tccagctggg acagcacgaa ccactgcgcc tgccgggacg
     6901 ggatgttgcg ctggggcacc gcctggtggt cggggttggg agcccgcagg ttgtgcagga
     6961 cgctgcgggc gaggcccttc gcgatcggga tcttgcgcgt gggcggcggc gggaacacct
     7021 gcgccgcgag tgcgtcgagc tccgacaacg gaacctgcgt ggccgagtcg agcggcctac
     7081 cgtcgtcgta cttggcgcgc tcggcacgca cctcgcccag caccgtcgcc agcttcggga
     7141 acagcgcctc cggcccggcg aggaagtcgc gcagcgccat cgcctgcagc gcgacagccg
     7201 agtactccat cagcaacagg tgccgcagcg tgcgcttgat cgtgtcgcgc agcaacgcct
     7261 tcgggttgtc cgggccgtgc aacgcggccg cgacgaaccg gttgcggtag tggaagtagg
     7321 cctgccagtc actcgtgtcg tccttctcga ggaacgacat gtgccagatc gcgatacccg
     7381 gcaccgtggc cgtccggtag ccgcgcgagc gggcccggag gccgtactcg gcgtcgtccc
     7441 acttaatgaa caggggcagc ggcagcccga ggtcctcggc gacggcacgc gggatcaggc
     7501 acatccacca cgcgttgtag tcgacgtccg tacgccggtg cagccagggg gtctggcgca
     7561 gcgtgcggtc ggccaggtca tggtgcggct ccgtgccagg ggcattgcgc cagaggaagg
     7621 tgttgcggtc gacgacctcg cccatggtgg acagctgcga gcgggcctgc agcgacagca
     7681 tctgcccgcc gacgagcatc ggttcgcggg agaaccggga gaacgcgacg gcccgcagca
     7741 cagagtcggg ttcgagcagg atgtcgtcgt ccatgaagag gatctgctcg cagtcggtgc
     7801 tgccgagcgc ctcgtacatg acgcgtgcgt atccgcccga gccgccgaga ttgggctggt
     7861 cgatgatgcg gagcttgtcg ccgagcaccg cggccgcagc ttcgaagccg ggctcgtcac
     7921 gcaccttctt ggtgccctgg tcgggcagaa tcaccgcggt gacagcgtcg agcacgatcg
     7981 ggtcctcgcc gatggcggtg agggtggcga cgcagtccgc aggccggttg aacgtcggca
     8041 tgccgatcgt gacggcggcc cgcccggtgg ccgcctccgc cgcgtaccac ccgcccgagt
     8101 gcagggtgag ctcgctcgag tcggtggtga ggtcgaacca gtaccacccg ccgtcctcga
     8161 acgggcgcag gtccaggtcg aggtcgagtt cctggagtcc cgggccggtg atcacgatgc
     8221 cgcgcacgaa gatctgggag ccgtcggact tcgtccgata gacgtcgacg cggcccgtgc
     8281 cctcgactac cagccgcagg tgcagctccg tcagccggct ccagcgccgc cagtagcccg
     8341 ctgcgaacgc gttgaagtac gccccgaatg acacctcggt ctgctccggc accacggctg
     8401 agatgcgcga ggtgacccgc agccgccgga cacccatggc cgtgccggtg agatcgacct
     8461 tcgggccggg gggcgccccg gtcggctccg gcacggtggt catccggagc cccgtgcgct
     8521 catccaggta gagggagcga acgctcatcg ggtcacccga acgcgggagg atcacccgct
     8581 gcagcaggct gctggcggcc gcctccccgg cgggggtggc ggtctcctcg gcggtgacga
     8641 cggtctgggt catcgagtca ggccctgcat ggctgagttc agtcctcgct gctctcggtg
     8701 ccggccagcg cgcggccgtc ggtgaagtag ggggcgatgc gattgtcgaa catgctgagc
     8761 gccgatccga tcgccatgtg catgtcgaga tacttgtacg ttcccagcct gccgccgaac
     8821 aggacgttcg cgttggcggt ctccttgcgg gccagatcgc ggtagcgctg cagcttgacg
     8881 cggtcatccg gcgtgttgat cgggtagtac ggctcgtccc ccgactcggc gaaccgggag
     8941 tactcccgca cgatcactgt cttgtccgtc gggtaggcgc gctccgggtg gaagtggcgg
     9001 aactcatgga tccgcgtgaa cgggacgtcc tcgtcgttgt agttcatgac cggcgtgccc
     9061 tggaagtcgc cggtgtcgtg gcggacctcc tgctcgaagt ccagggtccg ccagccgagc
     9121 tcaccctcgg cgtagtcgaa gtaacggtcc aacgctcccg tgtagaccgt cggcgtgccg
     9181 gtcgggatct ggtcgcgcac gtcgaagtag tcgacgtcga gccgaacctc gacattcggg
     9241 tggtcggcca tcttctcgag ccacgctgta tagccgtcga ccggcaaacc ctcgtaggtg
     9301 tcgttgaagt accggttgtt gaacgtgtac cgcaccggca accgcgcgat gatcgacggg
     9361 tcgagcttgg tcgggtcggt ttgccactgc ttcgcggtgt agcccttgac gaaggcctcg
     9421 tagagcggac gccccaccag tgagacggcc ttctcctcga ggttcttcgc gtccttggtg
     9481 tcgatctcgc ccgcctgctc cgcgatcagg gcacgggcct cgtccggcgt gtgcgaccgg
     9541 ccgaagaact ggttgatcag cgccaggttc atcgggaacg cgtagacctg ctccccgacc
     9601 cgcgcgaaca cccggtgctg gtagtcggtg aagtccgtga agcggttgac gtactcccac
     9661 acccgctcgt tcgaggtgtg gaacaggtgc gccccgtaac gatggatctc gatgccggtc
     9721 tcgggttcgg gctcggagta ggcgttgccg ccgatgtgcg aacgccgctc gagcaccagg
     9781 acgcgcttgt ccagctcgga ggcggaccgc tcggcgacgg tcaggccgaa gaagcccgat
     9841 cccacgatga cgaggtcgta tcccgggtaa ctcacgactg ccgagggtag cggcggctgc
     9901 ctgacgacgc gggcgcaccc cgcctacgtg acatcgcgac gccgttgctg ggtggctgca
     9961 gacggcgcgt gtaacttcgc caggctgcga cgagtcgccg acggcggcga tgcggggggt
    10021 gtccgaaacg tggaagcagg agcggcgggc gccgggatcc atgtcgtggc gctctacgtc
    10081 gtcgtgctct gggtgccggg cctgctcctc ggctggctcg ccggaatgcg cggctggact
    10141 ctcgccgcgg gcgccccgct gctgacctac accgtcgccg gtctcgccgg tccggcgttc
    10201 tccgcagcca ggatctcctg gacgccggcg agtgcaggcg tcctgctggc catcctgacc
    10261 gcgggggcag cggccctgcg ctggtccgcg cgacggcgca acggtgcaac ggaggacgac
    10321 gtcgaagccc actgggcagg ccgggtccac ctcgcggttg ccgcggcgct gaccgcggtc
    10381 gtcgtcttcg gcgccgtcgt catctggacc ggtatcggcc gcctgtctgc cgtgccgcag
    10441 gactgggatg cggccttcca cgccaacggc atccggtgga tcgccgacac gggcgacagc
    10501 agtctcgtgg ggatgggcag cgtcaactgg tacgaggacg gcgtccacgt ctactacccc
    10561 aacgcctatc acctggtggc ggccacggtc ctccggttga cctccgccgg cataccgacc
    10621 gtcctcaacg cccagaccgt gcttctgccc ggaatctgcg cactcgtcgt cgtcgcgctc
    10681 gtgcaccggt tgcgtggccg ggcggtgctc gcggtggccg ccgcggcgtg cagcgtgtcc
    10741 atcacctcgt tctacgacat gctgtggcgg ggccctctac tgccgttcgt caccggcgcc
    10801 gcgctgatgc cgctggtcgc cgtgctggtg ctcgatctgc tcgacgccgg caccgggcgg
    10861 tcccgaatgg cgcccggcct gctgttcgcg tcagggttgg tcgggttgat ctgcttgaac
    10921 ccggcgacct tgttcagtgc ggtcctgttc gtggtgccgg cgctgggaca gcgttgggca
    10981 agccggccga gcctgctacg cagggagccg ctcgtcatcc tcggcgcagg tgtcgtggcg
    11041 gcggtgctgg cgctcccgca gctgctggcc tcactgggca gcgcggaggg ggcgccgttc
    11101 gattggcctg ccaccctgtc gcagttggag gccgtgggtc agctgctcac gctgtcccac
    11161 ggcgggctgc ccccccagtg gtgtctggtg gtcgcggcgg cagtggggct cgcgaggtac
    11221 gggaacctct cgcagctgcg atggatcggt gttgcgggcg ctgccttcgg ggcgctgttc
    11281 gtcctgtcgg catcctcgga cgcgttctgg gtgaacgcca tcacgcggcc gtggtggaac
    11341 gaccgctggc ggttgatcgg actgtgcgtg gttccgatcg cggtggtggc cggccatggt
    11401 ctggccgagc tgcagcgatc gaccgccgcc gcgctgggcg cggccgcgcg gatgtcgcga
    11461 ggcgatagca aggtgacggc gggtgtcagc agagcggcac ccatcgttgc ggtgggaatc
    11521 gtcggcgctt tgttcgtcgg cgcatcgaat gacctgtacg tcgaccggaa cgtcgcgcgc
    11581 atgcgtctga gcgcaccgga tggacctgtc gtgtcgtccc tggaggtcga tgccatgcat
    11641 gcgctcgcgg cagttgtgcc tgccgggcag cgggtgctca acgaccgcgg cgatggctcg
    11701 gcctggatgt acgcgatcgc gggagtgctc ccggttgcgg ggcactacaa cgcgtcgcgg
    11761 atcggccccg atgcgcagct gctcgccgac cggttcaacc gttatcccga cgatcccgcc
    11821 gtccaggcgg cgatcgagcg gctcaacatc tcttacgtga tgctcgacag gggcttcgtc
    11881 cggtcgagct ggtcgcgagc tcctggcctg gtcggtctgg acggcgccag ctggctggac
    11941 aaggtctacg aaaacccgga cgccgtcatc taccggatcc ggtcgggcat ccccagtcgt
    12001 ccctagtccg acccgacgct cacctgaggc tagggttccg cccgatgtcc gggaactggt
    12061 ccgtgggaac gttcgccgac gaacccggag cgaggccgct cgagcacgtg tccgccgagc
    12121 cgtcgcccac ctcgaggccg gcgagcaccg gtggacgcgc cggactccgg cggctcgccg
    12181 tccgggcggg cgaggcggca gctgccctgg gtgcggcggt cgtcgcggtg cagctcagcc
    12241 ggtccatcat ggtcgatccc ctggaccgcg tcgggcaggt cagcggggtt gccggtctcg
    12301 acctgcgctt cgtcgtgctc gggcttctgg tcctggcggc ctgtgccgct gcgacccgaa
    12361 ggccggcggc atgggcgatc gtcagccggt tcgcgtgcgc cgcggttgcc ggcctcgcca
    12421 ccggcctcgt cgcgggcggc atcctgctcg cgctgcgcgg aacgcagtgg ccgctgtacg
    12481 ccaactgggg cgactccgga cagctgatca ggtgggccga cgacctcctc gcaggacggc
    12541 cggtcccggc cgactac
//
LOCUS       DASRSC010000006         5753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1967255,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000006 DASRSC010000000
VERSION     DASRSC010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1967255"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..890
                     /locus_tag="VFE39_00185"
     CDS             15..890
                     /locus_tag="VFE39_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HET6257046.1"
                     /translation="MRDDLLRDLIHSRVHRPQAVAEAAARRRRPGSLLAEHGKLMMIA
                     ADHPARGALRAGGQVLAMADRGDLLDRLCLALSRPGVNGVLGTPDVLEDLLLLGALDG
                     KVVVGSMNRGGLAGTAFEIDDRFTAYDAASIAASGFEGGKMLIRIDPEDPATVATLEA
                     GARAVGQLAQHRLMAMVEPFISHRVDGRVRNELTAEAMIRAACVAAGLGATSAYTWLK
                     VPIVDEMERAMAATTLPALILGGEVTEDQDAAFASWSKALALPTVQGLVIGRSLLYPP
                     GDDVAGAVDAAVGLL"
     gene            926..1795
                     /gene="iolB"
                     /locus_tag="VFE39_00190"
     CDS             926..1795
                     /gene="iolB"
                     /locus_tag="VFE39_00190"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HET6257047.1"
                     /translation="MVRRGETADGTFSLVITPETAGWGYSSLRVLELPAGGSAAFDTG
                     EDEMVVLPLSGACAVECDGERFELAGRASVFSRVTDFAYVPRDAQVTVSSTAGGRFAL
                     PGARATRRLTARYGPADGVAVELRGAGQASRQVNNFCTPEAFDADKLIAVEVLTPAAN
                     WSSYPPHKHDEEREGEAQLEEVYYFEVAGGPAGEGVAYQRVYSSGPGREIDVCAEVSS
                     GDTVLIPHGWHGPSMAAPGYDLYYLNVMAGPSPDRAWLICDDPAHAWVRGTWGDQEID
                     PRLPLTTTEERSR"
     gene            1792..3729
                     /gene="iolD"
                     /locus_tag="VFE39_00195"
     CDS             1792..3729
                     /gene="iolD"
                     /locus_tag="VFE39_00195"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HET6257048.1"
                     /translation="MTIRLTVAQALVRFLANQYSERDGVEQRLIPGCFGIFGHGNVAG
                     VAQALLEAALQDGIGSAALPYHLARNEQGMVHSAVGYSRMRNRLQAMACTASIGPGST
                     NMLTGAALATTNRIPVLLLPSDIFATRVASPVLQELEQPWGYDVSVNDAFRPLSRFFD
                     RVWRPEQLPSALVGAMRVLTDPAETGAVTIALPQDVQAEAYDWPEELFARRVWHIPRP
                     VPEPAALARAAAVIRSARRPLLVAGGGVHYSEAAEALRRFAEATGIPVADTQAGKGAL
                     LWDHPQAVGGVGSTGSPVANALARDADVVIGVGTRYSDFTTASRTAFQNQDIHFVNIN
                     IAAFDAAKHSGTMVVADARSALDALVDAVEGYRVDDAYTAGHRAHAADWNQVVDESFH
                     LGHQPLPAQTEVLGALNEELAARDVVVQAAGSMPGDLQMLWRARDAKQYHVEYAYSCM
                     GYEIAGALGIKMAARSDPSGDREVFALVGDGSYLMMSQELVTAVSEGIKLNVVIVENR
                     GFASIGALSESVGSQRFGTAYRYRNPESGQLDGEVLPVDLAANAASLGADVIRVRTIE
                     EFRAALARSRASDRTTAIHIETDPLAPVPSSESWWDVPVSEVARLDSTNQARKTYEAH
                     KAEQRPLLRPSAEPRSEEGSR"
     gene            3726..5222
                     /locus_tag="VFE39_00200"
     CDS             3726..5222
                     /locus_tag="VFE39_00200"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016335519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET6257049.1"
                     /translation="MRTITHWIGGKPEPGTSTRTSPVWNPATGEQQAEVTLASVADVD
                     AAVRSAAVAFETWSQSSLSTRTTVLFAFRELVNARTQELAEIISDEHGKVVSDARGEV
                     QRGLEVVEFACGIPTLLKGDFSDQVSGGVDVFSFRQPLGVCAGITPFNFPAMVPMWMH
                     PVAIACGNTFVLKPSERDPSASMLVAELWAEAGLPDGVFTVLHGDKEAVDGLLDHPDV
                     AAVSFVGSTPIARYIHERGTANGKRVQALGGAKNHAVILPDAPLDYASDHLVAAAFGS
                     AGERCMAISAAVAVGPAADDLVAAVSEKARAVKVGPGRETDSEMGPVVTAAARDRIVG
                     LIGSGAEQGATLAVDGRGLTVPGHEKGFFVGPTVIDNVTREMDVYREEIFGPVLSVVR
                     ADSVDEAIALINANPYGNGTAIFTSSGEAARRFQRGVNVGMIGINVPIPVPMAYYSFG
                     GWKDSLFGDKHVHGPEGVSFYTRAKVVTARWPHVEAVSGASYHFPTAN"
     gene            5234..>5753
                     /locus_tag="VFE39_00205"
     CDS             5234..>5753
                     /locus_tag="VFE39_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET6257050.1"
                     /translation="MTEHVRERSAGRGLSRLRLGTAPDSWGVWFADDPHQVSWKQYLD
                     EIAQAGYLYTELGPAGFLPQDPAQLRDELSARGLTLCGGTIFAGLHRGAEALDKAIED
                     CGRESRLLTALGAKHLVLLPEQYTDMHTGTATESGSIDPEQWKNLTTGYSQLGRILRE
                     EFGVELVFHPHAD"
BASE COUNT          821 a         2086 c         2033 g          813 t
ORIGIN      
        1 gggggagacc atccgtgcgt gacgacctgc tccgcgacct gatccacagc cgcgtgcacc
       61 ggccccaggc cgtcgccgag gccgcggccc ggcggcgcag gccgggctcc ctgctcgccg
      121 agcacgggaa gctgatgatg atcgccgcgg accaccccgc caggggcgcg ctgcgggccg
      181 gtggtcaagt gctggccatg gccgaccgtg gcgacctgct cgaccggctg tgcctcgccc
      241 tgtcccggcc aggggtcaac ggcgtgctgg gcacaccgga cgtcctggag gacctcctgc
      301 ttctcggcgc cctcgacggc aaggttgtcg tcggctcgat gaaccgcggc gggctggccg
      361 gcaccgcatt cgagatcgac gatcgcttca ccgcctacga cgcggcgagc atcgccgcat
      421 ccgggttcga gggcggcaag atgctcatcc ggatcgaccc cgaggatccc gcgacggtcg
      481 ccacgctcga ggccggggcc cgagccgtcg gccagctggc gcagcaccga ctgatggcga
      541 tggtcgagcc gttcatctcc caccgagtgg acgggcgggt gcgcaacgag ctcaccgccg
      601 aggccatgat ccgtgcagcc tgcgttgcgg cagggctggg agcgacgtcc gcctacacgt
      661 ggctgaaggt gccgatcgtc gacgagatgg aacgggccat ggctgcgacg acgctgcccg
      721 cgctgatcct cggtggcgag gtgaccgagg accaggacgc cgcgttcgcg agctggagca
      781 aggcgctggc cctgcccacc gtccaggggc tggtgatcgg ccgttccctg ttgtacccac
      841 cgggtgacga cgtggcaggt gcggtcgacg cggcggtggg cctcctgtga gcgagctgct
      901 gagcgagacg ctgacggtag gccccgtggt ccgcaggggc gagacggccg acggcacctt
      961 ctccctcgtc atcactccgg agacggcggg ctgggggtac agcagcctgc gcgtgctcga
     1021 gctgcccgcc ggcgggagtg ccgctttcga cacgggcgag gacgagatgg tcgtcctccc
     1081 gctgtcgggt gcgtgcgccg tggagtgcga cggcgagcgt ttcgagctgg ccgggcgcgc
     1141 gagcgtgttc tcccgggtca ccgacttcgc ctacgtcccg cgggacgcgc aggtcaccgt
     1201 gtcgtcgacg gccggcggcc ggttcgcgct gcccggggcc cgcgccaccc gccggctcac
     1261 cgctcggtac gggcctgccg acggcgtcgc cgtggagctg cgcggggccg ggcaggccag
     1321 ccggcaggtc aacaacttct gcacgccgga ggcgttcgac gccgacaagc tcatcgccgt
     1381 cgaggtgctc acacccgccg ccaactggtc gtcctaccca ccccacaagc acgacgagga
     1441 gcgcgagggc gaggcgcagc tcgaggaggt ctactacttc gaggtcgcgg gcggcccggc
     1501 cggcgaaggt gtcgcctacc agcgcgtcta ctcctccggg ccgggacgcg agatcgacgt
     1561 gtgcgcggag gtcagcagcg gcgacaccgt gctcatcccg cacggctggc acgggccgtc
     1621 gatggccgcg cccggctacg acctctacta cctcaacgtc atggccggcc cgagcccgga
     1681 ccgggcctgg ctcatctgcg acgacccggc ccacgcctgg gtccgcggca catggggcga
     1741 ccaggagatc gacccccgcc tgccgctgac gacgaccgag gagcgctcgc gatgaccatc
     1801 cggttgaccg tcgcccaggc actggtccgg ttcctggcga accagtacag cgagcgtgac
     1861 ggtgtggagc agcggctgat ccccggctgc ttcggcatct tcggccacgg caacgtggcc
     1921 ggcgtcgcgc aggcgctgct cgaggccgcc ctgcaggacg ggatcggctc cgccgccctc
     1981 ccgtaccacc tggcgcgcaa cgagcagggc atggtccact ccgcagtcgg ctactcccgg
     2041 atgcgcaacc ggctccaggc gatggcctgc acggcgtcga tcgggccggg gtcgacgaac
     2101 atgctcaccg gcgcggcgct cgccacgacc aaccgcatcc cggtgctgct gctgccctcc
     2161 gacatcttcg ccacgcgggt tgccagcccg gtgctgcagg agctggagca gccctggggc
     2221 tacgacgtct cggtgaacga cgcgttccgg ccgctgtcgc ggttcttcga tcgggtctgg
     2281 cgccccgagc agcttccgtc ggcgctcgtc ggggcgatgc gggtgctcac cgacccggcc
     2341 gagacgggcg ccgtgacgat cgcgctgccg caggacgtgc aggccgaggc gtacgactgg
     2401 cccgaggagc tgttcgcgag gcgggtctgg cacatcccgc gtccggtgcc ggagcctgcc
     2461 gcgctggccc gggccgccgc cgtcatccgg tcggcccgcc gtcccctgct cgtcgcaggt
     2521 ggcggcgtgc actacagcga ggccgccgag gcgctgcgcc gcttcgccga ggccaccggt
     2581 atccccgtcg ccgacacgca ggccggcaag ggcgctctgc tgtgggacca cccgcaggcc
     2641 gtcggcggtg tgggctccac cggttcgccg gtcgccaacg cgctggcccg cgacgccgac
     2701 gtcgtgatcg gcgtcggcac ccggtacagc gacttcacca ccgcatcgcg gacggcgttc
     2761 cagaatcagg acatccattt cgtcaacatc aacatcgccg ccttcgacgc ggcgaagcac
     2821 tccggcacga tggtcgtcgc cgacgcccgg tcggcactcg acgcgctcgt cgacgcggtg
     2881 gaggggtacc gcgtcgacga cgcgtacacc gcgggccacc gggcgcacgc cgcggactgg
     2941 aaccaggtcg tggacgagtc cttccatctg ggtcaccagc ccctcccggc ccagaccgag
     3001 gtgctcggcg cgctcaacga ggagctcgca gctcgcgacg tcgtggtgca ggcagccggc
     3061 tcgatgcccg gcgacctgca gatgctctgg cgggcacgcg acgcgaagca gtaccacgtc
     3121 gagtacgcct actcctgtat gggctacgag atcgccgggg ccctcgggat caagatggcc
     3181 gctcggtccg atccttctgg ggatcgcgag gtgttcgccc tggtcggcga cggttcctac
     3241 ctgatgatgt cgcaggagct ggtcaccgcg gtgtccgagg ggatcaagct caacgtcgtc
     3301 atcgtggaga accgcgggtt cgcctcgatc ggcgcgctct cggagtcggt gggctcccag
     3361 cgattcggca ccgcctaccg ctaccgcaac ccggagtccg ggcagctgga cggcgaggtg
     3421 ctcccggtcg acctcgccgc caacgccgcg agcctcggtg ccgacgtcat ccgggtccgg
     3481 acgatcgagg agttccgcgc cgctctggcg cgttcccgcg cgtccgaccg caccaccgcg
     3541 atccacatcg agaccgaccc gctcgcgccc gtcccgtcgt cggagagctg gtgggacgtg
     3601 ccggtgtccg aggtcgcccg gctcgacagc accaaccagg cccgcaagac ctacgaggcg
     3661 cacaaggccg agcagagacc gctgctgagg ccgtccgccg aaccccgttc cgaggaggga
     3721 tcgcgttgag gaccatcacg cactggatcg gcggcaagcc ggagccgggc acgtccaccc
     3781 gcaccagccc ggtgtggaac cccgccaccg gggagcagca ggccgaggtg acgctggcga
     3841 gcgtggcgga cgtcgacgcg gccgtccggt ctgcggccgt cgccttcgag acgtggtcac
     3901 agtcctcgct gtcgacccgt accacggtgc tcttcgcgtt ccgggagctg gtgaacgctc
     3961 gcacgcagga gcttgccgag atcatctccg acgagcacgg caaggtggtc tccgacgctc
     4021 gcggtgaggt gcagcgcggc ttggaggtcg tcgagttcgc ctgcgggatc ccgaccctgc
     4081 tcaagggcga cttctccgac caggtctccg gcggcgtcga cgtcttctcg ttccgccagc
     4141 cgctcggcgt ctgtgccggc atcaccccgt tcaacttccc ggccatggtg cccatgtgga
     4201 tgcacccggt ggccatcgcc tgcggcaaca cgttcgtgct caagcccagc gagcgcgacc
     4261 cgagcgcgtc gatgctggtg gcggagctgt gggccgaggc aggcctgccc gacggcgtgt
     4321 tcaccgtgct gcacggcgac aaggaggccg tcgacgggct gctcgaccac ccggacgtcg
     4381 ccgcggtgtc gttcgtgggg tcgacgccga tcgcgcggta catccacgag cgcggcacgg
     4441 cgaacgggaa gcgcgtgcag gccctcggcg gcgcgaagaa ccacgcggtc atcctcccgg
     4501 acgccccgct cgactacgcc tcggaccacc tcgtcgcggc ggcgttcggc tcggcgggcg
     4561 agcgctgcat ggcgatctcc gcggccgtcg ccgtcggccc cgcagccgac gacctcgtcg
     4621 ctgccgtgag cgagaaggcc cgcgcggtca aggtcgggcc aggacgcgag accgacagcg
     4681 agatgggccc tgtcgtcacc gccgcggccc gcgaccgcat cgtcgggctg atcggcagcg
     4741 gcgcggagca gggcgccacg ctcgccgtcg acggccgcgg cctcacggtt cccggccacg
     4801 agaagggctt cttcgtcggc cccaccgtga tcgacaacgt cacgcgggag atggacgtct
     4861 accgcgagga gatcttcggc ccggtgctct cggtcgtgcg cgccgactcc gtggacgagg
     4921 cgatcgcgct gatcaacgcc aacccgtacg gcaacggcac cgccatcttc acctccagcg
     4981 gggaggcggc ccggcggttc cagcgcgggg tgaacgtcgg catgatcggc atcaacgtcc
     5041 cgatccccgt gccgatggcc tactactcct tcgggggttg gaaggactcg ctcttcggcg
     5101 acaagcacgt ccacggcccc gaaggggtgt cgttctacac ccgcgcgaag gtcgtcaccg
     5161 cgaggtggcc gcacgtcgag gcagtcagcg gtgccagcta ccacttcccc acggccaact
     5221 gaggatcccg gccatgaccg agcatgtacg ggaacgatca gcagggcgcg ggctctcccg
     5281 gctccggctc gggacggcac ccgactcgtg gggcgtctgg tttgccgacg acccccacca
     5341 ggtctcgtgg aagcagtacc tcgacgagat cgcgcaggcc ggctacctct acaccgagct
     5401 cggaccggcg gggttcctgc cgcaggaccc ggcacagctg cgcgacgagc tgtcggcgcg
     5461 cgggctgacg ctgtgcggcg gcacgatctt tgccggtctg caccgcggcg ccgaggcgct
     5521 ggacaaggcg atcgaggact gcggccggga gtcccggctg ctcacagccc tcggtgcgaa
     5581 gcacctggtc ctgctcccgg agcagtacac cgacatgcac accggcaccg ccaccgagtc
     5641 cggctccatc gaccctgagc agtggaagaa cctgaccacg ggctactcgc agctcggccg
     5701 gatcctgcgc gaggagttcg gggtggagct cgtcttccac ccgcacgccg aca
//
LOCUS       DASRSC010000007         5826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3932885,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000007 DASRSC010000000
VERSION     DASRSC010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5826
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3932885"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..361)
                     /locus_tag="VFE39_00210"
     CDS             complement(<1..361)
                     /locus_tag="VFE39_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET6257051.1"
                     /translation="MRDDLLRDLIHSRVHRPHAVAEAAARRRRPGSLLAEHGKLMMIA
                     ADHPARGALRAGGQALAMADRGDLLDRLCLALSRPGVNGVLGTPDVLEDLLLLGALDG
                     KVVVGSMNRGGLAGTAFE"
     gene            complement(354..1346)
                     /gene="iolC"
                     /locus_tag="VFE39_00215"
     CDS             complement(354..1346)
                     /gene="iolC"
                     /locus_tag="VFE39_00215"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047590 - 5-dehydro-2-deoxygluconokinase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HET6257052.1"
                     /translation="MTPGDRTGHGPQAVRSALEVLTMGRIGVDVYPLQVGVSLREVST
                     FGKYLGGSASNVAVAAARHGRRSAVITRTGQDPFGEFLHDALRGFGVDDRFVTAVPGL
                     PTPVTFCEIFPPDDFPLYFYRLPKAPDLQIRADELDMDAVRDADIFWVTGTGLSQEPS
                     REATLAALRARGRRGITVLDLDYRPMFWSSREEAHRFMQVALPLVTVAVGNLDECETA
                     VGVREPHAAALALHDAGVELAVVKQGPAGVLASDGTREVEVPPVPVEVVNGLGAGDAF
                     GGALCHALLSGWDLERAMRFCNAAGAIVAGRLACADAMPTTDEVEAELGETIRA"
     gene            1507..2226
                     /locus_tag="VFE39_00220"
     CDS             1507..2226
                     /locus_tag="VFE39_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6257053.1"
                     /translation="MALDRSSPVPLYFQVASRLQQLIESGVMPVGGRLENEVELADRL
                     AVSRPTIRRAVQYLVERGVLVRKRGVGTQIVHPKVRRPVELSSLYDDLTKSGQTPRTE
                     IRTLHVGAPADHVAEALGIGEDAEVTWIERLRFADGEPLAIMHNAVPVDVLPLRREDL
                     EERGLYELLRAAGYAPRMANQVVGARVATSAEARLLDDVRGAPLLTMTRTAWDASGRA
                     VEYGSHIYRASRYSFELTLST"
     gene            complement(2358..2564)
                     /locus_tag="VFE39_00225"
     CDS             complement(2358..2564)
                     /locus_tag="VFE39_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3073 domain-containing protein"
                     /protein_id="HET6257054.1"
                     /translation="MGRGRAKAKQTKVARELKYSSPTMDLDALQREIGSGTAPRNGGA
                     EDDYVDPYAPEADDEDEAPSRRYR"
     gene            complement(2809..4005)
                     /locus_tag="VFE39_00230"
     CDS             complement(2809..4005)
                     /locus_tag="VFE39_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6257055.1"
                     /translation="METGTRYRWVVLAVGTAAQAATATYFLGLAAVTPALRDHFGLGL
                     AAVGTLIGAISVGLVATLIAWGGTADRFGERGVMTVGLVGAAVALGGAALVRDPVVAG
                     MLLLLAGASGASVNAASGRAVLTWFPAERRGMAMAVRQTSVPVGAALAAVALPPIAEA
                     SGVPAVFATLALTCLVAAGAVAIWVREPPGRPARGTRPATSALTVLADRRLLRLSLAG
                     LLLVVPQFLGSVFLVEVLHEGAGLPLGLAGGLLALTQVLGALGRLGNGVWSDRVASRL
                     GPLRVVAVASAVGFAVAAALEPGPAPLLAAVLVPAAALAISWNGLVFTAAGELAPPDR
                     AATAMAVSNTANYAAAAVTPALGGLVAEVAGWPAMLAMGAVAALGAFVALIRLAEPVA
                     QRSASA"
     gene            complement(4101..5189)
                     /locus_tag="VFE39_00235"
     CDS             complement(4101..5189)
                     /locus_tag="VFE39_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HET6257056.1"
                     /translation="MSGPGQTLPGADSDPQVPAHRGDPLAEQRAMARAAAVVDRGHRG
                     VIAVTGDDRLSWLHLLLTQHVSELPADTGTEALVLDINGRVLHHMVVGHTRDTVYLDT
                     EPGEVPELLGYLQKMVFWSKVEPRDATGELAVLSVVGPETADVLAAAGVDVPAAPHGA
                     LALPDGGVARCMAWPGTDAADLLVPREAQAAWWARLTEAGARPAGTMAFEALRVEAMR
                     PRLHVDTDDRTIPHEVGWIGSAVHLTKGCYRGQETVARVANLGRPPRRLVLLHFDGGD
                     EHLPMPGDAVLREGRPVGRVGTVALHHELGPVALALVKRSVPVDAELVAGADDRATPA
                     AIDPTSVPPEDGAPPPGRAAQIRGGLRG"
     gene            complement(5186..5635)
                     /locus_tag="VFE39_00240"
     CDS             complement(5186..5635)
                     /locus_tag="VFE39_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET6257057.1"
                     /translation="MLDTARESSTLRRTLHQRGLRMTPQRQLVLDAVRALGHATPEQI
                     CAQVQRAAPAVNITTVYRTLDLMERIGLVRHTHLGHGAPTYSEQEHRHVHLVCHSCGA
                     VTETPTDLMDDLADRLRTETDFELDPSHVALSGICRACRDATPDELT"
BASE COUNT          833 a         2111 c         2091 g          791 t
ORIGIN      
        1 tctcgaacgc ggtgccggcc agcccgccgc ggttcatcga gccgacgaca accttgccgt
       61 cgagggcgcc gagcagcagg aggtcctcca ggacgtccgg tgtgcccagc actccgttga
      121 cccccggccg ggacagggcg aggcacagcc ggtcgaggag gtcgccacgg tcggccatgg
      181 ccagcgcctg gccaccggcc cgcagcgcgc cccgggccgg gtggtccgcg gcgatcatca
      241 tcagcttgcc gtgctcggcg agcagggagc ccggtctgcg ccgccgggcc gcggcctcgg
      301 cgacggcatg gggccggtgc acgcggctgt ggatcaggtc gcggagcagg tcgtcacgca
      361 cggatggtct cccccagctc ggcctcgacc tcgtcggtgg tcggcatggc gtcggcacag
      421 gcgagccgac ccgccacgat ggcgcccgca gcgttgcaga agcgcatcgc ccgttcgagg
      481 tcccacccgg acaggagcgc gtggcacagc gcgccaccga atgcgtcgcc cgccccgagg
      541 ccgttcacca cctcgaccgg caccggcggc acctccacct ctcgcgtgcc gtcggaggcc
      601 agcacgcctg ccgggccctg cttgacgacg gccagttcga cgccggcgtc gtgcaacgcc
      661 agcgcggcgg cgtgcggctc gcggaccccc accgccgtct cgcactcgtc gaggttgccc
      721 accgccaccg tcacgagtgg gagcgccacc tgcatgaagc ggtgcgcctc ctcccgggag
      781 gaccagaaca tcggccggta gtcgaggtcg aggacggtga tgccgcgcct gccccgtgct
      841 cgcagggcgg cgagcgtcgc ctcgcggctc ggctcctgcg agagcccggt gccggtcacc
      901 cagaagatgt cggcgtcgcg cacggcgtcc atgtccagtt cgtcggcgcg aatctgcagg
      961 tcaggcgcct tcgggaggcg gtagaagtag agcgggaagt cgtcgggcgg gaagatctcg
     1021 cagaacgtga ccggggtcgg caacccgggc acggccgtga cgaaccggtc gtcaacgccg
     1081 aagccgcgca gggcgtcgtg caggaactcg ccgaacgggt cctggccggt ccgcgtgatc
     1141 accgcgctcc gcctgccgtg gcgggcggcg gcgacggcca cgttggatgc gctgccgccg
     1201 aggtacttgc cgaacgtgct cacctcgcgg agcgacaccc cgacctgcag cggatagacg
     1261 tccaccccga tgcggcccat cgtgagcacc tccagggcgc tccggaccgc ctgcggcccg
     1321 tgcccggtgc ggtcccccgg cgtcatcgct ctcctcccgt tcgggtcatg cgctccggaa
     1381 gtgaccccgg acaccgagac agatgtactc tggcccgcgg aacctgtcaa cactttgtcc
     1441 tgacatagcc cctgtggagg agtgcaagcg ttggagcgtt cgactccccg gggtctggtc
     1501 ccggaggtgg cgttggaccg cagcagcccg gtcccgcttt acttccaggt cgcgagcagg
     1561 ctgcagcagc tgatcgagag cggcgtgatg ccggtcggtg gccggctcga gaacgaggtg
     1621 gagctggccg atcgcctcgc cgtctcgcgg ccgacgatcc ggcgtgccgt ccagtacctc
     1681 gtcgagcgtg gcgtgctggt ccgcaagcgg ggggtcggca cgcagatcgt ccacccgaag
     1741 gtccgcagac ctgtcgagct gtcgagcctc tacgacgacc tgaccaagtc ggggcagaca
     1801 ccgcgtaccg aaatccggac gctgcacgtg ggggcgccgg cggaccacgt ggcggaggcg
     1861 ctcggcatcg gcgaggacgc cgaggtgacg tggatcgagc ggctgcgttt cgcggacggc
     1921 gagccgctcg cgatcatgca caacgcggtg ccggtcgacg tgctgccact gcgccgcgag
     1981 gacctcgagg agcgcgggct ctatgagctg ctccgagcgg ccgggtacgc gccgcgtatg
     2041 gccaaccagg tcgtgggagc aagggttgcc acctcggccg aggccaggct gctcgacgac
     2101 gtccggggtg caccgttgct cacgatgacg cgcacggcgt gggacgccag tggcagggcc
     2161 gtggagtacg gctcgcacat ctaccgcgcg agccgctact ccttcgagct gaccctgtcc
     2221 acctgacgca tgccgcctac cgggtcagat cccccgttac ggaggctcga cccgctgccg
     2281 gaccacgtcc tcgacccgat gcagccgagg gagcggcccc ggactgactg acggtgcgat
     2341 ggcccggaaa ccaggcgtca ccggtaccgg cggctcgggg cttcgtcctc gtcgtccgcc
     2401 tcgggggcgt acgggtcgac atagtcgtcc tcggccccac cgttgcgggg tgccgtccca
     2461 ctcccgatct cgcgctgcag cgcgtcgaga tccatggtgg gggagctgta cttgagctct
     2521 cgggccacct tcgtctgctt ggccttcgct cgtccgcgcc ccatggctgg tcccccttac
     2581 aacagggaag gggcggccgg ataactcggc ggccccattc acatctcatc aatgtcctgg
     2641 tgaccaccgt accttgctca cggcctcgtg tgcgatgtgg caccagttgt gtgacttcca
     2701 acgcctcggg gcctgtcggc ggtgctggca ccgctgcttc cggtacccgc aacggcctct
     2761 gccaagtccg caggtgaagc ccctgacgcg cggtccggcg gcccctcctc atgcactggc
     2821 gctccgctgc gccacgggct cggccaaccg gatcagcgcg acgaacgccc ccagtgcggc
     2881 gacggcgccc atcgccagca tcgcgggcca gccggcgacc tcggcgacca gtccgcccag
     2941 cgcaggcgtc accgccgcgg cggcgtagtt cgccgtgttg gacacggcca tcgcggtggc
     3001 ggcacggtcg ggcggggcga gctcgcccgc tgcggtgaag acgaggccgt tccagctgat
     3061 cgcgagcgcg gcggcgggca cgaggacggc ggcgagcagt ggtgccggtc ccggttccag
     3121 cgccgcggcc accgcgaacc cgacggccga cgcgaccgcg acgacgcgca gtggccccag
     3181 cctgctcgcc acgcggtcgg accacacgcc gttgccgagc cggcccagtg ctcccagtac
     3241 ctgggtgagc gccagcagcc cgcccgccag gccgagcggc aggccagcac cctcgtgcag
     3301 aacctcgacg aggaacacgg agccgaggaa ctgcgggacg acgagcagga gcccggcgag
     3361 gctcagtcgc agcagtcgcc ggtcggcgag gacggtcaag gcgctggtgg cgggccgggt
     3421 gccacgggcg ggccggccgg gcggttcgcg cacccagatc gccacggcgc ccgcggcgac
     3481 gagacaggtg agggcgagcg tggcgaacac cgccgggacg cccgatgcct cggcgatcgg
     3541 cgggagggcg acggccgcga gcgcggcccc gaccggcacc gacgtctgtc gcacggccat
     3601 cgccatcccc cggcgctccg cggggaacca ggtgagcaca gcccgaccgc tggccgcgtt
     3661 gacgctcgct ccggaggccc cggcgagcag cagcagcatg ccggcgacga ccgggtcgcg
     3721 gacgagcgca gcgccgccca gcgccacggc tgccccgacc agtcccacgg tcatcacgcc
     3781 gcgctcaccg aaccggtccg cagtgccacc ccaggcgatc agggtggcca ccagaccgac
     3841 ggagatcgct ccgatcagcg tcccgacggc ggcgaggccg agcccgaagt ggtcacgcaa
     3901 cgccggtgtg acggcggcca gaccgaggaa gtaggtggcc gtggcggcct gcgccgcggt
     3961 gcccaccgcg agcacgaccc accggtaccg agtgcccgtc tccacgtgat cgaccgtacg
     4021 tcgccgtctc gccaggcggg agcattatcc caaaaagtgg gacaatggac gcggtggcgt
     4081 tcatgagccg gaccgcgaaa tcagccccgc aggccgccgc ggatctgggc cgcgcgtccc
     4141 ggcggcggcg cgccgtcctc cggcggtacc gaggtggggt cgatcgcagc gggcgtcgcc
     4201 cggtcgtcgg ccccggccac caactcggcg tccaccggca ccgaccgctt gaccagcgcc
     4261 aatgccaccg gcccgagctc gtgatgcagt gccacagtgc cgaccctgcc gacgggacgg
     4321 ccctcgcgca gcaccgcgtc gcccggcatc ggcaggtgtt cgtcgccgcc gtcgaagtgc
     4381 agcagcacga gtcgacgcgg cgggcgtccg aggttggcca cccgtgcgac cgtctcctgt
     4441 ccgcggtagc accccttcgt caggtgcacg gccgagccga tccagcccac ctcgtgcggg
     4501 atggtgcggt cgtcggtgtc cacgtgcagc cggggccgca tggcctccac acgaagggcc
     4561 tcgaacgcca tcgtgcccgc cggccgcgcg cctgcctccg tcaaccgggc ccaccacgcg
     4621 gcttgggcct cccggggcac cagcaggtcg gcggcatcgg tgcccggcca cgccatgcac
     4681 cgcgccacgc cgccgtccgg caacgcgagc gcaccgtgcg gtgccgccgg cacatccaca
     4741 cctgccgcag cgagcacgtc ggccgtctcc ggcccgacca cgctcagcac cgccagctcg
     4801 cccgtggcgt cgcggggctc gaccttcgac cagaacacca tcttctgcag gtagccgagc
     4861 agctccggga cctcaccggg ctccgtgtcg agatacaccg tgtcccgggt gtgcccgacc
     4921 accatgtggt gcagcacgcg cccgttgatg tcgagcacga gtgcctcggt gccggtgtcg
     4981 gccggcagct cgctgacgtg ctgggtgagc aacaggtgta gccagctcaa ccggtcgtcg
     5041 cccgtgacgg cgatgacgcc acggtgcccg cggtcgacca ccgcggcggc acgggccatc
     5101 gccctctgct cggccaacgg gtcgccgcgg tgggcgggta cctggggatc ggagtcggca
     5161 ccggggagtg tctgtcctgg cccgctcatg tcagctcgtc cggggtggcg tcgcggcagg
     5221 cacggcagat cccggagagg gcgacgtggg aggggtcgag ctcgaagtcc gtctcggtgc
     5281 gcagtcggtc ggccaggtcg tccatcaggt cggtgggcgt ctccgtgacc gcgccgcagg
     5341 agtggcagac gaggtggacg tgccggtgtt cctgctcgga gtaagtgggc gcgccatggc
     5401 cgaggtgggt gtgccgcacg agcccgatgc gttccatgag atcgagggtt cggtagaccg
     5461 tggtgatgtt gacagcgggc gcggctcgct gcacctgcgc gcagatctgc tccggcgtcg
     5521 cgtggccgag cgcgcggacg gcgtcgagga ccagctgccg ctgtggggtc atgcgcaggc
     5581 cgcgctggtg caatgttcgt cgtagcgtcg aggattcacg cgcggtgtcg agcatcgacg
     5641 gagtctccac cggttcgatg gtaggccgtc ccgtctccgc tgtccgaatg ctggtgggac
     5701 cggtacccgc gatccggcga cgagtgaaac cgtccactat cggtatgctc cgtcagggtg
     5761 aacgaacagg cttgaccgcg acgatctacc taccctcggc gatatgcacg gtgacagtac
     5821 gctcgg
//
LOCUS       DASRSC010000008        11460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2415, whole
            genome shotgun sequence.
ACCESSION   DASRSC010000008 DASRSC010000000
VERSION     DASRSC010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11460
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2415"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VFE39_00245"
     CDS             complement(<1..1059)
                     /locus_tag="VFE39_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HET6257058.1"
                     /translation="MTGRGALIIDGKSLSCQDVVHASQRSTSVSLAPDAEQAAVRANQ
                     VADELSRIRPMYGRTTGVGANRDAVVISSAAGDHDLRLLLSHAPGVGEPLDEISVRAA
                     MLVRLNQLAAAGSGIHPHFLHALEQALQTDAVPTVHDAGAIGTGDLTALAEIALTLSG
                     HLPWASGHVDPIGLEPGDALAFMSSNAVTLARAVLVREELRRLLDAAHLVTALSYCAM
                     GGSAEAFAEEVHARRPHPGAVRSARTMRELLWHEGDLSAGRRIQDPFGLRAFCQVHGP
                     ALDSTEALERVLEIEVNAAAENPLVDVAGRAVFHHGQFSTAYPALALDQLRAAVHHVA
                     ELSAARLADLVEPDFTGLT"
     gene            complement(1056..2285)
                     /gene="hutI"
                     /locus_tag="VFE39_00250"
     CDS             complement(1056..2285)
                     /gene="hutI"
                     /locus_tag="VFE39_00250"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HET6257059.1"
                     /translation="MTVLYTDIGELVTCDPARGDGSPLGVISDAAVIVDDSFIVWTGP
                     ARHAPAADHRVRCHGQTLIPGFVDSHAHLVFAGHRAEEFAARANGRAYAAGGIHSTVE
                     ATRAATDAELAATVDGLAGEMLAAGITTFECKSGYGLTVRDELRALQIATRATPETTY
                     LGAHVVPTEYQGRRDAYIDLVRGPMLDACAPHARWIDVFCEQGAFDVEESRAILEAGR
                     AHGLRARLHSGQLGFGGGVQLAVELGAASVDHATFLTDDDVAALAAGSTVATLLPGVE
                     FSTRTAWADGRRLLDAGVTVALATDCNPGSSYTSSMAFCLALAVRHQGLTPAEALHAA
                     TVGGASALDRHDVGAIAVGHRADLVRLDAPTYVHLVYRPGVPLVREVVRRGVRYDVHR
                     RPTRLPWPRRTSEGAPS"
     gene            complement(2282..3658)
                     /locus_tag="VFE39_00255"
     CDS             complement(2282..3658)
                     /locus_tag="VFE39_00255"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HET6257060.1"
                     /translation="MSGQWWCEHAWTGPGATRRGVLVAVEHGRITRVVADVDHPPDGV
                     EVLRGLVVPGLANAHSHAFHRLLRSRTQRDRGSFWTWRELMYRAAHQLDPTTYYELAA
                     AVFTEMALAGITTVGEFHYLHHGRDGTAYSDPNAMGAALVDAARTAGIRLTLLDTCYL
                     SSEVDRTPLRPAQRRFGDGSGDAWAARVDTLHQRFGAADDVVIGAALHSVRAVPLEHI
                     PAVTAWAQAHSAPLHVHVAEQTAEVECCRAVHGCSPVALLRDAGVLGPRTTAVHATHL
                     NPADIVDLRASGTGVCFCPTTERDLGDGVGPAPALLTAPSGPVSLGSDSHAVVDLFEE
                     ARAVELDERLWRHQRGVIDADTLLRCATEHGHRALGRTDAGTITPGARADLIAIDLAT
                     VRTAGGGATAETAVFAATAADVSDVVIDGRVVVRDRRHLTRPEPGRDLARATAALLDD
                     PGPWECVS"
     gene            complement(3655..4872)
                     /locus_tag="VFE39_00260"
     CDS             complement(3655..4872)
                     /locus_tag="VFE39_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HET6257061.1"
                     /translation="MNTFDALWADIAEVGRDPHTGGYRRFVLGEADLMLREWFIGCAT
                     TRGMDVERDHNGNLWAWWMPEGSSGDPRGAFVTGSHLDSVPDGGAFDGPLGIVAAFAA
                     IDALRGEGHRPRVPVGVVAFSEEEGARFGVACVGSRLSTGALAPARALALRDAGGRTL
                     AEVAAGAGIDPDRMGRDDRVADRAAVFVELHIEQGRALDRLGQPVAVASSIWPHGRWQ
                     LTFRGQADHAGTTLLPDRHDPMVPYAATVTAARDGAFAHGGVATFGKVQVAPNGANAI
                     ASAVTAILDARAPSQIVLEAMTAELFDVATHHADHEGTELTVSTESLSRRVDFPEAPR
                     DRILAALGDLPVLPTAAGHDAGVLATEVPTAMLFVRNPTGVSHSPREWAEHADCLSGV
                     AALTGAMREWTKP"
     gene            complement(4869..6533)
                     /gene="hutU"
                     /locus_tag="VFE39_00265"
     CDS             complement(4869..6533)
                     /gene="hutU"
                     /locus_tag="VFE39_00265"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HET6257062.1"
                     /translation="MSESPPRPAIRAQRGPVLRCRGWQQEAALRMLENNLDPEVAEHP
                     EELVVYGGSGRAARSWAAFDAIVDCLHQLADDETLLVQSGKPVGVLQTHEWAPRVLLA
                     NANLVGDWADWTQFRRLEAAGLTMYGQMTAGSWIYIGSQGILQGTFETFAAVARKRFG
                     GSLAGTITLTAGLGGMGGAQPLAVTMNEGVAICIDSDPTRIDRRISHRYLDVRADDLD
                     DALRRALEARDARRPVSIGVVGNAAAVLPELLRREAPIDVVTDQTPAHDPLAYLPLGV
                     EVGEMGAAAAADPAGYTERSRASMATHVEAMIGFLDRGAEVFDYGNSIRAEARHGGAK
                     RAFDIPGFVPAYIRPQFEEGRGPFRWAALSGDPADIAATDRAVLELFPGDEALQRWIT
                     IAGERVAFEGLPARICWLGYGERHRAGLRFNGMVASGELSAPVVIGRDHLDAGSVASP
                     YRETEAMLDGSDAIADWPLLNALTNTSSGASWVSIHHGGGVGIGRSQHAGQVCVADGT
                     ELAARKLDCVLRNDPATGVLRHVDAGYPHARQVARERGLRIPMEGR"
     gene            6639..7454
                     /locus_tag="VFE39_00270"
     CDS             6639..7454
                     /locus_tag="VFE39_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET6257063.1"
                     /translation="MPSSASPAVSRTLDVLLHLASRPRPIPGAALIRDLGLPRSTAYH
                     LLTVLTERGFVVHYPDQHAYGLGVASFEIGSAYLRQGSLEHLGRPLARRLARLSGTIA
                     HLGILHGADSLYLVKERPASRTRPPSLVTEVGVRLPASLTANGRSILAHVGSAQRRAL
                     FPAPGSLVLRTDSGPATLGELEDLLDSERDRGWSWEHGMITEGMESVGACAFDHTGLA
                     VAAFSVTRRADPAAPRPDDLAALVRSSAARLTRTLSGSAPPGWFRTGGRPGEP"
     gene            7667..9205
                     /gene="hutH"
                     /locus_tag="VFE39_00275"
     CDS             7667..9205
                     /gene="hutH"
                     /locus_tag="VFE39_00275"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HET6257064.1"
                     /translation="MRDVVIGVGPVGPQELVAVARDNAVVRLSGEAVAAIGKSREVVE
                     SLAEETRTHYGVSTGFGALATRHIPATLRSQLQAGVVRSHAAGSGSEVEPEVVRALML
                     LRLSTLATGRTGVRVETAQAYAELLNAGLSPVVGEYGSLGCSGDLAPLAHCALAVMGE
                     GEVRDRAGHRLPAAEALAAHGIEPVVLAVKEGLALINGTDGMLGMLVLACHDLRGLLW
                     TADVAAAMSVEALLGTDAAFAADLQNLRPHPGQADSAANLVALLAGSPIMASHRGPEC
                     TRVQDAYSMRCAPQVHGAARDTLAHAERVAAVELASAIDNPVVTSDGRVASNGNFHGA
                     PLAYVLDFLAIAVADVASIAERRTDRLLDVARNHGLPAFLADDPGVDSGHMIAQYTQA
                     AIVSELKRLAAPASVDSIPSSAMQEDHVSMGWSAARKLRRALDGLGRVLAIEALTAAR
                     ALDLRAPLTPAPATAAAVARLRSEVPGPGADRHLAPEIESARGQVISGALVAAAESVT
                     GPLR"
     gene            complement(9662..9865)
                     /locus_tag="VFE39_00280"
     CDS             complement(9662..9865)
                     /locus_tag="VFE39_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257065.1"
                     /translation="MLRITAIGAGAVDYLLRGCDDPAHDHRELADRADSAELGADRYF
                     AKVMAGPDLVGRRERRGLSDPGA"
     gene            9983..10462
                     /locus_tag="VFE39_00285"
     CDS             9983..10462
                     /locus_tag="VFE39_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257066.1"
                     /translation="MHAAINRARAELVRPAAEHQRDLAGDHLDALTARAWAVVDDPGP
                     VVSAGKVVLDAEGRSIRDRRVLLEALKILVMIDERRARLVGLDAPRRQAVEVVTNEVV
                     DAEIAELTQKLANREAARGRPGRDRERAAVVTGVRIELGQHQSLAGDGLLELPVLRR"
     gene            10759..10914
                     /locus_tag="VFE39_00290"
     CDS             10759..10914
                     /locus_tag="VFE39_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257067.1"
                     /translation="MTIARSVVDLARLPAAGRHHVPAAPDRSPQQLAALLVGPVDKAR
                     QRLRAAM"
     gene            complement(11167..>11460)
                     /locus_tag="VFE39_00295"
     CDS             complement(11167..>11460)
                     /locus_tag="VFE39_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007035253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HET6257068.1"
                     /translation="PGPVPGPDQVLAGHLATAAATGIVHHRAQREVGTVNEQLKHALA
                     SRIIIEQAKGVLAERHRLDLAVAFLRLRSHARSNNQRLANVAQAVVEGTLDMQ"
BASE COUNT         1555 a         4010 c         4297 g         1598 t
ORIGIN      
        1 cgtcaggccg gtgaagtcgg gctcgacgag gtcggccagg cgagcggccg agagctcggc
       61 cacgtggtgc accgcggcgc gcagttggtc gagggccaag gccgggtagg cggtggagaa
      121 ctggccgtgg tggaagactg cccgtccggc gacgtcgacg agggggttct cggccgccgc
      181 attgacctcg atctcgagca cccgttccag cgcctcggtg ctgtccagtg cggggccgtg
      241 gacctggcag aacgcccgga gcccgaacgg gtcctggatg cgacgaccgg cgctcaggtc
      301 gccctcgtgc cagagcagct cgcgcatcgt ccgggctgag cgcaccgcac cggggtgtgg
      361 acgacgggcg tggacctctt cagcgaatgc ctccgcggag ccgcccatgg cgcagtacga
      421 gagtgcggtc acgaggtgcg cggcgtcgag caatcgacga agttcctccc tgaccagtac
      481 ggcccgcgcg agggtgaccg cgttggagga catgaacgcg agcgcgtcgc cgggctcgag
      541 gccgatcggg tcgacgtgcc cagaggccca cggcaggtgg ccgctcagcg tgagtgcgat
      601 ctcggccagc gccgtgaggt cgccggtgcc gatggcgccg gcgtcgtgca cggtgggcac
      661 cgcatcggtc tgcagcgcct gctcgagcgc gtgcaggaaa tggggatgga taccgctgcc
      721 ggcggccgcg agctggttga gtcgcaccag catcgctgcc cgcaccgaga tctcgtccag
      781 cggctccccg acccccggag cgtggctcag cagcaggcgc aggtcgtggt caccggctgc
      841 gctgctgatc actaccgcgt cgcggttggc gccaaccccg gtggtgcggc cgtacatcgg
      901 ccggatccgg ctcagctcgt ccgcaacctg attcgcgcgg acggcggcct gttcggcgtc
      961 cggagctaac gacaccgacg tggaccgttg ggaggcgtgg accacgtcct ggcagctgag
     1021 cgactttcca tcgatgataa gtgcgcctcg gccggtcatg acggtgctcc ttccgacgtt
     1081 cggcggggcc acggaagccg ggtcggccgg cggtggacgt cgtatcggac ccctcgacgc
     1141 accacctcgc gcaccagtgg gacaccgggt cggtagacca ggtggacata ggtcggggcg
     1201 tctagccgca caagatcagc tcggtgcccc acagcgatcg cgccgacgtc gtgacgatcc
     1261 agtgccgagg cgccgccgac ggtcgcggcg tgcagtgcct cggcgggggt taggccctgg
     1321 tgacgcacgg cgagggccag gcagaaggcc atgctcgagg tgtaagacga tcctggattg
     1381 cagtcggtgg ccagcgccac ggtcacgccg gcgtcgagca gccggcggcc gtcggcccag
     1441 gcggtgcggg tgctgaactc gacgccgggc aacagggtgg ccacggtcga tccggcggcc
     1501 agtgcggcga cgtcgtcgtc ggtcaggaag gtcgcgtgat ccaccgacgc cgcgccgagc
     1561 tcgaccgcca gctggacgcc gccgccgaac ccgagctggc cggagtgcag tcgggcacgc
     1621 agcccgtgcg cccgtcccgc ctcgaggatg gcccgggact cctcgacgtc gaatgcgccc
     1681 tgctcgcaga acacgtcgat ccagcgggcg tggggggcgc atgcgtcgag catggggccg
     1741 cggacgaggt cgatgtaggc gtcgcgccgc ccctggtact cggtgggcac gacgtgggca
     1801 ccgaggtagg tggtctccgg cgtcgcccgg gtcgcgatct gcagcgcgcg gagttcgtcc
     1861 cgcacggtga ggccgtagcc gctcttgcac tcgaaggtgg tgatcccggc ggcgagcatt
     1921 tcgccggcga ggccgtcgac tgtcgccgcg agctccgcgt cggtcgcggc gcgcgttgcc
     1981 tcgacagtgc tgtggatgcc accggccgcg taggcgcggc cgttcgcgcg ggcggcgaac
     2041 tcctcagcgc ggtggcctgc gaacacgagg tgagcgtggc tgtcgacgaa tccggggatc
     2101 agggtctgtc cgtgacaccg gacgcggtgg tcggccgccg gtgcgtgacg cgcgggtccg
     2161 gtccagacga tgaagctgtc gtcgacgatc accgccgcgt cggagatgac gccgagcggg
     2221 ctgccgtcgc cgcgcgcggg atcgcaggtg acgagctcgc cgatgtcggt gtagagcacg
     2281 gtcacgacac gcactcccaa ggcccggggt cgtcgagcag tgccgcagtc gcacgggcca
     2341 ggtcgcggcc cggctcgggg cgggtgaggt gtcgtcgatc gcggactacc acgcggccgt
     2401 cgatgacgac gtcgctgacg tcggcggccg tggcggcgaa gacggcggtt tcggctgtgg
     2461 caccgccgcc ggcggtccgg accgtcgcca gatcgatcgc gatcagatcc gctcgcgcgc
     2521 cgggggtgat cgtcccggcg tcggtgcggc cgagcgcacg gtgcccatgc tcagtcgcgc
     2581 accggagcag ggtgtcggcg tcgatgacgc cgcgttggtg gcgccacagc cgttcgtcga
     2641 gctcgaccgc gcgggcctcc tcgaagagat cgacgacggc gtggctgtcc gagcccaggc
     2701 tgaccggacc ggatggcgca gtgagcaggg cgggcgccgg gccgacgccg tcgcccaggt
     2761 cgcgctccgt ggttgggcag aagcagacgc cggtgcccga ggcgcgcagg tcgacgatgt
     2821 cggcggggtt caggtgggtc gcgtgcacgg cggtcgtgcg ggggcccagg acgcccgcgt
     2881 cacgcagcag cgccacggga ctgcatccat gcaccgcgcg gcagcactcg acctccgcgg
     2941 tctgctcggc gacgtgtacg tgcagcggag ccgagtgcgc ctgcgcccac gcggtcaccg
     3001 ccgggatgtg ctcgagcggc acggcgcgca ccgaatggag ggccgcgccg atcaccacgt
     3061 cgtcggcagc gccgaaccgc tggtgcaggg tgtcgacgcg cgcggcccag gcgtcgccgg
     3121 agccgtcgcc gaagcgccgt tgggcgggcc gtagcggtgt gcggtccacc tcggaactca
     3181 gatagcaggt gtcgagcagg gtgagccgga ttccggcggt ccgggcggcg tcgaccaagg
     3241 cggcgcccat cgcgttgggg tcgctgtacg cggtgccgtc gcggccgtgg tggaggtagt
     3301 ggaattcgcc gaccgtggtg atcccggcga gtgccatctc ggtgaacacc gccgcggcga
     3361 gttcgtagta ggtcgtcggg tcgagctggt gggcagcgcg gtacatgagc tcgcgccagg
     3421 tccagaacga gccgcggtcg cgttgggtcc gggagcgcag cagccggtgg aaggcgtgcg
     3481 agtgcgcgtt ggccagaccg ggcacgacca gcccgcgcag cacctcgacg ccatcgggtg
     3541 ggtggtcgac gtcggcgacc acccgggtga tgcggccgtg ctcgacggcg acgagtacgc
     3601 cgcggcgggt cgccccggga ccggtccagg cgtgctcgca ccaccactgg ccgctcacgg
     3661 tttcgtccac tcgcgcatcg cgccggtcag ggcagccacg ccggacagac agtccgcgtg
     3721 ctcggcccat tcccgcgggg agtgcgagac gccggttgga ttgcgcacga acagcatggc
     3781 ggtggggacc tcggtggcca gcaccccggc gtcgtggccg gcggcggtgg gcagcacggg
     3841 caggtcgccg agcgcggcga ggatgcggtc gcgcggggcc tccgggaagt cgacccgccg
     3901 ggagagcgac tcggtgctga ccgtgagctc ggtgccctcg tggtcggcgt ggtgggtcgc
     3961 gacgtcgaag agctcggcgg tcatcgcctc gagcacgatc tgactcgggg cgcgggcgtc
     4021 gaggatcgcg gtcaccgcgg aggcgatggc gttggcgccg ttcggggcca cctgcacctt
     4081 gccgaaggtc gcgacaccgc cgtgcgcgaa cgcgccgtcc cgtgcggcgg tgacggtcgc
     4141 cgcgtagggc accatcgggt cgtgccggtc ggggagcagg gtcgtgccgg cgtggtcggc
     4201 ctggccccgg aacgtcagct gccaccgacc gtgcggccag atcgaggaag ccaccgccac
     4261 cggctggccc agccggtcga gcgcgcggcc ctgctcgatg tggagctcga cgaacaccgc
     4321 ggcgcggtcc gcgacccgat catcgcggcc catccggtcc gggtcgatcc cggcgcccgc
     4381 cgcgacctcg gcgagcgtcc gccctccggc gtcgcgtagg gccagcgctc gcgccggggc
     4441 cagcgcaccc gtcgagagcc gggaaccgac acaagcgacc ccgaagcggg cgccttcctc
     4501 ctcgctgaat gcgacgactc cgacgggcac ccggggccgg tgaccctcgc cgcgcaacgc
     4561 gtcgatcgcc gcgaacgccg cgacgatccc cagggggccg tcgaaggcgc cgccatcggg
     4621 caccgagtcc aggtgcgatc cggtgacgaa cgccccccgc ggatcaccgg acgagccctc
     4681 gggcatccac catgcccaca ggttgccgtt gtgatcgcgc tcgacgtcca tcccacgggt
     4741 cgtggcgcaa ccgatgaacc actcgcgaag catcaggtcg gcctcgccaa gcacgaagcg
     4801 gcggtacccg ccggtgtgcg ggtcgcggcc cacctcggcg atgtccgccc agagggcgtc
     4861 gaaggtgttc accgaccctc catggggatc cggaggccgc gctcccgggc gacctgccgg
     4921 gcgtgtggat agccggcgtc cacgtggcgc aggaccccgg tcgccgggtc gttgcgcagc
     4981 acgcagtcga gcttgcgggc ggcgagctcg gtgccgtcgg cgacgcagac ctgacctgca
     5041 tgctgggagc ggccgatacc gaccccgccg ccgtgatgga tcgacaccca cgacgctcct
     5101 gacgaggtgt tggtgagcgc attgagcagc ggccagtcgg cgatcgcgtc ggagccgtcg
     5161 agcatcgcct cggtctcccg atacggcgag gccaccgacc ctgcatcgag atggtcgcgc
     5221 ccgatcacca cgggggcgct gagttctccc gatgcaacca tcccgttgaa ccgcagtccg
     5281 gcccggtgcc gctcgccgta cccgagccaa cagatgcgcg cgggcaggcc ctcgaatgcg
     5341 acccgctcgc cggcgatggt gatccagcgc tgcagcgcct cgtcgcccgg gaacagctcg
     5401 agcaccgccc ggtcggtggc ggcgatgtcg gccgggtcgc cggagagcgc cgcccagcgg
     5461 aacgggccgc ggccctcctc gaactgcggg cggatgtagg cgggcacgaa acccggaatg
     5521 tcgaacgctc gtttcgcgcc gccgtggcgg gcctcggcgc ggatggagtt gccgtagtcg
     5581 aacacctcgg cgccgcggtc gaggaagccg atcatcgcct cgacgtgggt ggccatcgac
     5641 gcgcgtgaac gctcggtgta gccggccggg tcggcggccg ccgcggcgcc catctcgccc
     5701 acctcgacgc cgaggggcag gtaggccagt gggtcgtggg cgggtgtctg gtcggtgacg
     5761 acgtcgatgg gtgcctcccg gcgcagcagc tcggggagca cggccgcagc gttgccgacc
     5821 acgccgatcg acaccggacg gcgggcgtcg cgcgcttcca gggcacggcg cagggcgtcg
     5881 tcgaggtcgt cggcgcgcac gtcgaggtag cggtgcgaga tccggcggtc gatacgggtg
     5941 ggatcggagt cgatgcagat cgcgacgccc tcgttcatgg tcaccgcgag cggctgggca
     6001 ccgcccatgc cgccgagacc cgcggtcagg gtgatggtgc cggcaagcga gccgccgaac
     6061 cgcttgcggg cgaccgccgc gaaggtctcg aaggtgccct gcaggatgcc ctggctgccg
     6121 atgtagatcc acgagcccgc cgtcatctgg ccgtacatcg tcaggccggc ggcctcgagt
     6181 cgacggaact gggtccagtc cgcccagtcg ccgaccaggt tcgcgttggc cagcagcacc
     6241 cggggagccc actcgtgggt ctgcagcacc ccgaccggtt tgcccgactg gacgagcagt
     6301 gtctcgtcgt cggcgagctg gtgcaggcag tcgacgatgg cgtcgaacgc cgcccagctc
     6361 cgggccgcgc gcccggagcc cccgtacacc actagctctt cggggtgctc ggcgacctcg
     6421 ggatcgaggt tgttctccag catccgaagc gcagcttctt gctgccagcc acggcagcgc
     6481 agcacggggc cgcgttgggc cctgatggcg ggacgcggcg gggactcgga catcgctact
     6541 cctgggcttc cggcgaacac cagtccatcg ccgggtcggt ggccagcgcc agggtcgcgg
     6601 cggagttagc gtctcggttc cgagactttg gagtcgtcgt gcccagcagc gcctcacccg
     6661 cggtcagtcg cacgctcgac gtgctgcttc acctcgccag ccggccccgg ccgatcccgg
     6721 gggccgcgct gatccgcgac ctcggactgc cccgctcaac ggcgtaccac ctgctcacgg
     6781 tcctcaccga gcggggtttc gtcgtgcact accccgacca gcacgcctac gggctgggtg
     6841 tcgcctcctt cgagatcggc agcgcctacc tcaggcaggg ctcgttggaa cacctcggcc
     6901 gtccgctcgc acgccggctg gcccgcctca gcggcaccat cgcgcacctc ggaatcctgc
     6961 acggagccga ctcgctgtac ctggtcaagg agcggccggc gagccgtacc cgcccgccgt
     7021 cactggtcac cgaggtcggt gtgcggctcc cggcctcgct caccgccaac ggtcgatcga
     7081 tcctggccca cgtgggctcc gcgcagcggc gcgccttgtt tccggccccg gggtcgctgg
     7141 tactgcgcac ggactccgga ccggccacgc tgggcgagct ggaggacctg ttggacagcg
     7201 agcgcgaccg cggctggtca tgggagcacg gaatgatcac ggaggggatg gagtcggtcg
     7261 gggcgtgcgc cttcgaccac accggcctcg cggtcgccgc gttcagcgtt actcgccgtg
     7321 ccgatccggc cgcgccccgg cccgacgacc tcgccgccct ggtgcgctcc agcgccgccc
     7381 ggctcacccg gactctgtcc ggctcggcgc caccgggctg gttccgtacg ggcggccgcc
     7441 ccggtgagcc gtgaggcgct cgtgcttcgc tggtcccggg cgtgcacatc agcggctctt
     7501 cgtcgcgaag cgcggaagcg gcctcgtaac atcgggggtg cgggagtgtt ccaggcgggt
     7561 cgagcaggtc atcggcctcg ctcgaggcga gcccacgtcg cggagccaag acgagattgc
     7621 gcctggggat gtggttgcgg gcggctccgt ggggtgtgct ggaggcatgc gtgacgtagt
     7681 catcggagtg ggacctgtcg gcccccagga gctcgtcgct gtcgcacggg acaacgccgt
     7741 tgtacgcctc tccggcgaag cggtcgccgc gatcggcaag agccgcgagg tggtcgagtc
     7801 gctggctgag gagacccgga cccactacgg ggtctcgacc ggcttcggag cgctggccac
     7861 tcgccacatc cctgcgacgc tgcgctcgca gctgcaggcc ggcgtggtcc gttcgcacgc
     7921 cgccggctct ggctcggagg tggagcccga ggtcgtccgg gccctgatgc tgttgcggct
     7981 gtccacactg gcgaccgggc ggacgggggt ccgggtggag acggcccagg catacgccga
     8041 gctgctcaac gcgggtctgt ccccggtggt cggcgagtac ggctcgctgg gctgctccgg
     8101 agatctggca ccgctggcgc actgcgcgct ggccgtcatg ggcgagggcg aggtccgtga
     8161 ccgcgcaggg caccggctac cggccgccga agcgctcgcg gcccacggga tcgagccggt
     8221 cgtgctcgcc gtgaaggaag ggctcgcgct gatcaacggc accgacggca tgctgggcat
     8281 gctggtgctg gcctgccatg acttgcgcgg gctgctatgg actgcggacg tggccgccgc
     8341 gatgagcgtc gaggcactgc tcggcaccga cgcggcgttc gcggccgatc tgcagaatct
     8401 gcgcccccat cccgggcagg ccgactccgc ggcgaacctg gtcgcgctgc tggccggctc
     8461 tcccatcatg gccagccacc gcggcccgga gtgcacccgc gtacaggacg cctactcgat
     8521 gcgctgcgcc ccgcaggtcc acggcgcggc ccgggacacc ctggcccacg ccgagcgggt
     8581 cgccgcggtt gagctggcgt cggccatcga caacccggtg gtcaccagcg acggtcgcgt
     8641 ggcgtccaac ggcaacttcc acggcgcccc gctggcctac gtgctcgatt tcctggcgat
     8701 cgcggtggcc gacgtggcct cgatcgccga gcgacgcacc gaccggttgc tcgacgtggc
     8761 ccgtaaccat ggcctgcctg cgttcctggc cgacgacccc ggcgtggact ccgggcacat
     8821 gatcgcccag tacacacagg cagccatcgt ctccgagctc aagcgtctgg ccgctccggc
     8881 ctcggtcgat tcgatcccgt ccagcgcgat gcaggaggac cacgtctcga tgggctggtc
     8941 ggccgcccgc aagctgcgcc gcgcgctcga cgggctggga cgagtgctgg cgatagaggc
     9001 gctgaccgcg gcccgcgcgc tcgaccttcg cgccccgctg accccggcac cggcgactgc
     9061 ggcggcggtg gcccgcctgc gcagcgaggt gcccggcccg ggcgccgacc gccacctcgc
     9121 acccgagatc gagtccgccc gcggccaggt gatctcagga gccctggtgg ccgccgccga
     9181 gtcggtcacc ggccccctgc gctgaactac gcagctcgag gggaaaggac accgacatgc
     9241 ccgacgggcg cgcctcacgc gccagcgaac ggcaacgcga ccgtagtctg aaggccccgc
     9301 tgcgggtgct gcagaacaac ctcgaccccg aggtcgcgga cgcccggaaa ttagctaccg
     9361 cggcacgtgc cgacgcggcc ggtccagggg gagccgccgg ctgttcggca cagcccggtc
     9421 accgcatggt cgaagatctt gccagctgac gaccacggca aacacgggac agcggagccc
     9481 ctctcacccg cgaacatgtg cgccggcgcc gccggcgtgg ccaccgcgtc cgcctgtcgc
     9541 gtgccatgag gctgggaccg gctgatccga aggtcgcgca gcgcccgtgt cgtcatcgcc
     9601 ccgggacggg ccagtcggct gctgtcgtga cagaagacct cgcggtcaca gtgcggccca
     9661 gtcaagctcc ggggtccgac agtcctcgcc gctcgcggcg tccgaccagg tcggggcccg
     9721 ccatcacctt cgcgaagtac cggtcggcgc cgagctcggc ggagtcggcc cgatcggcga
     9781 gctcgcggtg atcgtgtgcg gggtcgtcgc acccgcgtag gaggtagtcc acggccccgg
     9841 cgccgatggc ggtgatccgg agcatgaggc gacggtagat cctcgccagc cacagcgcga
     9901 cgcccgcgtc tactctctcc gcctccgcgg ttggacgtgg cgggagatcg gcgacgggct
     9961 gggtttctcg tcgatctccg gggtgcacgc cgcgatcaac agggcgcggg cggagctggt
    10021 ccgcccggct gcggaacatc agcgcgacct cgctggtgac cacctcgatg cgctcacggc
    10081 gcgcgcctgg gctgtcgtgg acgatccggg ccccgtggtg tcggcgggga aggtggtgct
    10141 ggacgcggaa ggccggtcga tccgagatcg gcgggtgctg ctggaagcac tcaagatcct
    10201 cgtgatgatc gacgagcggc gggcgcgctt ggtagggctc gacgcgccgc ggcggcaggc
    10261 cgtcgaggtg gtcaccaacg aggtggtgga cgccgagatc gccgagctga ctcagaagct
    10321 cgccaaccgc gaggccgcgc gcggtcggcc cggccgagat cgagagcggg cggccgtcgt
    10381 gacgggcgtc cggatagaac tgggacagca ccagtccctg gcgggcgatg gtctcctgga
    10441 gctcccggtt ctccgccggt agtacttccg tataccggtt ccgatgacgg ccacttaagc
    10501 aggcgcgcca catcgacgac atccccgcga ccgagcgcga cgaactcctc caggtaggcc
    10561 gcgaaccact gctgcacatt gccgtcgtcc acggctcgga tggtggcccg acggtggcgg
    10621 cgcgcgccag gacctgcctc gactggcgcc ttgcggaggt gcggcgctaa ctcacctcca
    10681 gcccgcgcag ggcgccgccg ccgaggacga cgacgtggac ggcgagctcg gcgggggtgt
    10741 gcgtccagtg cggccgcggt gaccatcgca cgcagcgtcg tcgacctggc ccgactgccg
    10801 gccgccggcc gccaccacgt gcccgccgcg ccggacaggt ccccacagca gctcgcggcg
    10861 ctgctggtag ggccggtcga caaggcccgc caacgcttgc gtgccgccat gtgaggccca
    10921 cacgacgcgt cgcgctccgt taaatggctt cagccgttcc ggcggtgtcc ggctaaaaga
    10981 tccgcaaaaa tgggtgaccg ctggcctgcc cctcatgacc gcgagcccct cgggcgtcga
    11041 cgaggacggc cgcccgaggg gctcgcgctg gggatgctcc ggcggtcgcg tcggcctcgt
    11101 cggcgggtgt gcgcagtcaa tgaaccggcg gacgctgcgc cgggcggctc gggcgggggc
    11161 tgggccctat tgcatgtcga gcgttccctc gacgacggcc tgggcgacgt tggccaggcg
    11221 ctggttgttg ctcctggcgt ggctgcgcag ccggaggaac gcgacggcga ggtccaggcg
    11281 gtggcgctcg gcgaggacgc ccttggcctg ctcgatgatg atccggctgg ccagcgcgtg
    11341 cttgagctgc tcgttgacgg tcccgacctc gcgctgagcg cggtggtgga cgatcccggt
    11401 cgccgcagcg gtcgcgaggt gaccggcgag cacctggtcg ggcccgggca cggggccggg
//
LOCUS       DASRSC010000009         8260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_821935,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000009 DASRSC010000000
VERSION     DASRSC010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8260
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_821935"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VFE39_00300"
     CDS             <1..712
                     /locus_tag="VFE39_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toluene hydroxylase"
                     /protein_id="HET6257069.1"
                     /translation="WVRFVERNVGAWMHIEHILGLYVFAACNRSGPTNMHNTAMVVNS
                     EHKIRFAQDLALYNLTLSEEIDGFDGAAHIDAWNNDAEWQGARNVCEALTAVQDDWGE
                     SIFATNIVFEPLVGELFRSNLVQQIAAGNGDFVTPTLVGAGEYDYAQRDLRWSMACFG
                     PLTQDWEFADHNKQLMQGWLAKWVPQCLEAARTLQPIWSQVDAKPPRFEDSLDWAKSR
                     FAGIVSDLGLETPKELKQ"
     gene            709..1080
                     /locus_tag="VFE39_00305"
     CDS             709..1080
                     /locus_tag="VFE39_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmoB/DmpM family protein"
                     /protein_id="HET6257070.1"
                     /translation="MTSYRTAESPYKSNNTASNMCGFTLMNNQVGALIANVVGRLDNV
                     TVTPLPSMIRVDAQGRFDTVYADIDEEAGEEEGWFDAAEFEENMSTHYGRMVHLDDRT
                     IMFANPEDAAEYLDFDLKPLT"
     gene            1199..2887
                     /gene="groL"
                     /locus_tag="VFE39_00310"
     CDS             1199..2887
                     /gene="groL"
                     /locus_tag="VFE39_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636926.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET6257071.1"
                     /translation="MAKELRFGADARHLLQAGVDQLAEAVKSTLGPKGRNVILEKITG
                     TPEVTNDGVTIAREIHLRDPFENMGAQLLKEAAIKTNDIVGDGTTTATVLAQAIVRQG
                     MKAIESGGNPVLVKRGIDVAVGALVARLAQVAHPVSTEEDYSRVAAISANDDDAIGRV
                     VARALHTVGNDGTVTVEDSPTIGLEVSFVEDFEFDNGYVSPYMVTDQGRLEAVLEDAY
                     LLFSAEKIKDVRSLMPVLEQVMRADRRPLVVIAETVEGTALQMLVHNHVNGVFQNVAI
                     KAPGFGEKRIHRLEDMAALCGGKVHSKSSSFSIEQVTIEHLGRAAQVRVTSDHTTIIG
                     GAGSREAVELRLTQLRAELERASIGTDEDFLAERIARLSGKAAIIWVGAPTNAEAKEV
                     RHRVDDSLQATRAAIAEGIVAGGGSALLHAEPALDELEVTGDYQIGVEIVRGALTEPV
                     HLIASNAGYDGDQVVKQVTAMAVDEGFDALEGRYGNMVEMGIIDPLRVVRSALQNGAS
                     VAGLILTTNSLVAEEVTPWNKALMTEFGPLDDGIPQPSPDSSTPQSLGLGPSVG"
     gene            2898..4016
                     /locus_tag="VFE39_00315"
     CDS             2898..4016
                     /locus_tag="VFE39_00315"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6257072.1"
                     /translation="MYTVGGQRILVVDAQVHVWDASPHNQAIPDGEAYATDLLRRHRE
                     LDGAGVPRHEVERVSEQSLTRDVFEAGYVDRAVLQPVVLGDLFVLGFSPLEWHAEMAG
                     WMPGRFVLSGELDPGGGQPGARGLGPRVRRFDLRGLTLCERRRPAGWQRLGDPWLRRT
                     LARCGAAGADVVHIGVGPSTLPAPWRREQAGAPAPPPGAARDRPPRVPRWAEPVRAGS
                     ALPARTRAAERVAPAGFDAGQFRTLARALPDIRFVLGAGCLPGPRLCRLAELPNVHVL
                     LTEILPWIGSPDFAHAFGQLLAAYGPERLLFGSGYPLVRPGRLVRELGAYRFPDELRH
                     RYPRFDTAAKRAVLGGNAARLYRIGRPVPTAPAVAAGA"
     gene            complement(4080..5426)
                     /locus_tag="VFE39_00320"
     CDS             complement(4080..5426)
                     /locus_tag="VFE39_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6257073.1"
                     /translation="MSVEGKRESVGQADDRTVRRAVAGAAMGNAVEWFDYAIYSYLVT
                     AIGLNFFPEADPTAQTMLVFAGIAIPFVLRPFGGVLLGPLGDKFGRRRVLAFTIIMMS
                     GATFCIGLIPSYHSIGIAAPVLLLLARLVQGFSTGGEYGGAATFIAEYAPDHRRGFYG
                     SFLEFGTLAGYVLGASLATAVQLGLPQEALLSWGWRIPFLIAGPLGLIGFYLRYRLED
                     TPAFRDAENAGETARAPFRECMQAVWPQILNLIGFVILLNVAVYTLLTYMPTYLTQVL
                     QISESESLLIIIGVMLGMMAVIAPVGALSDRIGRKPLLLASAIGFLLLSYPAIALIGM
                     RDPLPVTVGMVILALLLVLMLGTIGSALPAMFPTRNRYGGFSIGYSTSTAAFGGTAPL
                     IITALIDATGNTAIPAFYLMAAAAIAIVPILLMPETARVSISHPTAIPGTEPVPKR"
     gene            complement(5515..6564)
                     /locus_tag="VFE39_00325"
     CDS             complement(5515..6564)
                     /locus_tag="VFE39_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HET6257074.1"
                     /translation="MSWPDADFPADPYPGAVPPSSFLHTDEVSYRLRPDPRAGWLLGD
                     VPLDTWLAGHGSAPAAARVPVLTYGSNRCPSKITWLRRELGLGADPVVVLRATTRDLT
                     AVWAAGLRHRDGQRPAVLAASAGTVEDHAVWLATPEQIAVLDRCEGRDDRFRLARLRT
                     GEVRLEDGTRVDAPWCYLGHAATRRPLLVDGRAVRCVDLPQAAAAELTGDAAPDDGLD
                     ADTVHGEPHPDEWPAALFAYGLLQPGCPSWHRVAAHADGEPQRGAVDGTVLDTGFGYP
                     ALLPGTGGRAPGWVVPLRNPAAVLLALDDYEGVEYRRVRLAAAPGRQSCWAYAWSGPS
                     GGMTPLAAGWPPDPS"
     gene            6696..7670
                     /gene="mtnA"
                     /locus_tag="VFE39_00330"
     CDS             6696..7670
                     /gene="mtnA"
                     /locus_tag="VFE39_00330"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HET6257075.1"
                     /translation="MPRTIDWLDGRIALVDQTALPELRMVEIATVDELVDAIQRLVVR
                     GAPALGVAGALGVALAARTVAGAELAAVAARLAAARPTAANLAVGVRRAMAAATGGPE
                     AVLAAALAVLDEDVAACRAIGTRGAALLAELCGPAPLRLHTHCNAGALACVEWGTALG
                     VVRSLHEVGGVAHVVADETRPLLQGSRITAVELAAMGVPFRVVVDGAGASIIARGLVD
                     AVVVGADRVAANGDVANKIGTYPLAHAAARAGVPFVVAAPESTLDPDTPDGDAIEIEE
                     RAASEVLAFGGSAAAPAGSEVWNPAFDVTPADLVTAIVTEERVWRPRG"
     gene            7709..>8260
                     /locus_tag="VFE39_00335"
     CDS             7709..>8260
                     /locus_tag="VFE39_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6257076.1"
                     /translation="MLGATVLGFSGYSLLLPVVPLWAARGGAGALGAGATTAVLMLTT
                     VVTQLAVPWLLVRIGHRWVLGLGVALLGAPAPLLALSAHLGPVLAVSAVRGIGFGLAT
                     VAGSALVAELVPPAQHGRAVARYGLAVGLPQLALLAAGVAVVEQLGFAVVFVAAGVGP
                     MLGALLVPAIRVPRGEGRGVPTAV"
BASE COUNT         1220 a         2870 c         3022 g         1148 t
ORIGIN      
        1 ctgggtgcgc ttcgtcgagc gcaacgtcgg cgcctggatg cacatcgagc acatcctcgg
       61 gctgtacgtc ttcgccgcgt gcaaccgctc gggcccgacc aacatgcaca acaccgcgat
      121 ggtcgtgaac agcgagcaca agatccgctt cgcgcaggac ctcgccctct acaacctcac
      181 gctctccgag gagatcgacg gcttcgacgg cgccgcccac atcgacgcgt ggaacaacga
      241 cgccgagtgg cagggtgccc gcaacgtctg tgaggcgctc accgccgtac aggacgactg
      301 gggtgagtcg atcttcgcca ccaacatcgt gttcgagccg ctggtcgggg aactcttccg
      361 cagcaacctg gtgcagcaga tcgcggccgg caacggcgac ttcgtgacgc cgaccctcgt
      421 cggcgccggg gagtacgact acgcccagcg tgacctgcgc tggagtatgg cgtgcttcgg
      481 cccgctcacc caggactggg agttcgccga ccacaacaag cagctcatgc agggctggct
      541 ggcgaagtgg gtcccgcagt gcctggaggc cgcgcgcacg ctccagccga tctggtcgca
      601 ggtcgacgcc aagccaccgc ggttcgagga cagcctcgac tgggccaaga gccgtttcgc
      661 cggaatcgtg tccgatctcg gcctggagac cccgaaggag ctgaagcagt gacctcgtac
      721 aggacggctg agagccccta caaatcgaac aacaccgcgt cgaacatgtg cggtttcact
      781 ctgatgaaca accaggtcgg ggcgctgatt gccaacgtgg tgggccggct ggacaacgtc
      841 accgtcaccc cccttccgtc aatgatccgg gttgacgccc agggccgctt cgacacggtc
      901 tacgccgaca tcgacgagga agcgggcgag gaggagggct ggttcgacgc ggccgagttc
      961 gaggagaaca tgtccaccca ctacgggcgc atggtccacc tcgacgaccg cacgatcatg
     1021 ttcgccaatc ccgaggacgc ggccgagtac ctcgacttcg acctcaaacc cctcacctag
     1081 caccacctct cccgggtcgc cgcccgcaga tcagtctcgg gcggcggccc ggattcgccg
     1141 ttctgcaccc ccgcgcagcc gaccgacgaa acccgcgaac cgtccaggag gaccacccat
     1201 ggcgaaggaa ctgcgattcg gagcggacgc ccggcatctg ctccaagccg gcgtcgacca
     1261 actcgccgag gccgtgaaga gcacgctcgg gcccaagggc cgcaacgtca tcctggagaa
     1321 gatcaccggt acacccgagg tgaccaacga cggcgtcacg atcgcgcgcg agatccacct
     1381 gcgcgacccg ttcgagaaca tgggcgccca gctgctcaaa gaagccgcga tcaagacgaa
     1441 cgacatcgtc ggcgacggca ccactacagc caccgtgctt gcgcaggcca ttgtgcgcca
     1501 gggcatgaag gcgatcgaga gcggcgggaa cccggtgctg gtcaagcggg ggatcgacgt
     1561 cgcggtcggg gcactggtcg cccgcctcgc ccaggtcgcc cacccggtgt cgaccgagga
     1621 ggactactcc cgcgtggcgg cgatctccgc caacgacgac gacgccatcg gccgggtcgt
     1681 cgcgcgcgcc ctgcacaccg tcggcaacga cgggacggtc accgtggagg actccccgac
     1741 gatcgggctc gaggtgtcct tcgtcgagga cttcgagttc gacaacggct acgtctcccc
     1801 gtacatggtc accgatcaag gtcggctcga ggccgttctc gaggacgcct acctgctgtt
     1861 ttccgccgag aagatcaagg acgtccggtc cctgatgccg gtgctggagc aggtcatgcg
     1921 cgccgaccgc aggccgctcg tggtgatcgc cgagaccgtc gagggcacgg ccctgcagat
     1981 gctcgtgcac aaccacgtca acggggtgtt ccagaacgtc gcgatcaagg cgcccgggtt
     2041 cggggagaag cggatccacc ggctggagga catggccgcg ctctgcggcg gcaaggtgca
     2101 cagtaagagc tcgtcgttct cgatcgagca ggtgacgatc gagcacctgg gccgggctgc
     2161 gcaggtgcgc gtcacgagcg accacaccac gatcatcggt ggagccggga gccgggaggc
     2221 tgtcgagctg cggctcaccc agctgcgggc cgagctggaa cgagcgtcga tcggcaccga
     2281 cgaggacttc ctcgccgaac ggatcgcccg gttgtcgggt aaggcggcga tcatctgggt
     2341 cggcgctccg accaacgcgg aggccaagga ggtgcggcac cgcgtcgacg actcgttgca
     2401 ggccacccgc gccgcgatcg ccgagggcat cgtggcgggc ggtgggtccg ccctgctgca
     2461 cgcggagccg gcgctggacg agctcgaggt caccggcgac taccagatcg gcgtcgagat
     2521 cgtccggggc gcgctcaccg agccggtgca cctgatcgcc tccaacgcgg gctacgacgg
     2581 cgaccaggtt gtcaagcagg tcacggccat ggccgtcgac gaaggcttcg acgcgctcga
     2641 gggccgctac gggaacatgg tcgagatggg gatcatcgac ccgctgcggg tggtgcgctc
     2701 cgcgctgcag aacggcgcat cggtagccgg cctgatcctc accacgaact cgctggtggc
     2761 cgaggaggtc acgccctgga acaaggctct gatgaccgag ttcggaccgc tcgacgacgg
     2821 catcccgcag ccctcgcccg actccagcac gccgcagtcc ctcggcctcg ggccgtcggt
     2881 cggctagtac cggcgccgtg tacaccgtcg ggggtcagcg gatcctcgtc gtcgacgccc
     2941 aggtgcacgt gtgggacgcg agcccgcaca accaggcgat cccggacggc gaggcctacg
     3001 ccacggacct gctgcgcagg caccgggagc tcgacggtgc cggcgtaccg cggcacgagg
     3061 tggagcgcgt ctccgagcag agcctgacgc gcgacgtgtt cgaggccggg tacgtcgacc
     3121 gggcggtgct gcagccggtg gtgctgggcg acctgttcgt gctcggcttc agcccgctgg
     3181 agtggcacgc ggagatggcg ggctggatgc cgggccgctt cgtgctgagc ggcgaactcg
     3241 atcccggagg tgggcagccg ggtgctcgcg gtctcgggcc gcgagtgcgg cggttcgacc
     3301 tgcgtgggct cacgctctgc gagcggcgcc ggccggccgg ttggcagcgg ctgggggacc
     3361 cgtggctgcg ccgcaccctc gcgcgctgcg gcgcggccgg cgcggatgtc gtgcacatcg
     3421 gcgtcgggcc gtcgaccctg cctgcgccgt ggcggcgcga acaggccggt gcccccgccc
     3481 caccacccgg ggctgcgcgg gaccgcccgc cccgggtacc gagatgggcg gagcccgtgc
     3541 gggcgggctc cgcgctcccg gcccgcacca gggcggccga acgtgtggcg cccgccggat
     3601 tcgacgcggg gcagttccgc acactggccc gcgccctgcc ggacatccgg ttcgtgctgg
     3661 gtgcggggtg cctgcccggc ccacggctct gccggttggc ggagctgccc aacgtgcacg
     3721 tcctgctcac cgagatcctg ccgtggatcg gctcgccgga cttcgcgcac gccttcgggc
     3781 agctcctggc ggcgtacggg ccggagcggc tgctgttcgg cagcggctac ccgctggtgc
     3841 ggcccggacg gctggtgcgc gagctcggtg cctaccggtt cccggacgag ctgcgtcacc
     3901 gctacccgcg cttcgacacg gcagccaagc gagccgtgct cggcggcaac gcggcgcggc
     3961 tctaccgcat cgggaggccg gtccctaccg cacccgccgt ggccgctggg gcctagtaca
     4021 cctccccccg tgcgcggata cgagtgctag agcactcgta tccgcgcacg ggccctgtat
     4081 caccttttgg gcaccggctc cgtgccgggg atggccgtcg ggtgggagat cgagaccctg
     4141 gccgtctccg gcatcagcag gatcgggacg atcgcgatcg ccgcagcggc catgaggtag
     4201 aaggcgggga tggcggtgtt gccggtggcg tcgatcaggg cggtgatgat cagtggggcg
     4261 gtgccgccga acgccgcggt ggacgtggag tagccgatgg agaagccccc gtaacggttg
     4321 cgcgtcggga acatcgccgg caacgccgat ccgatcgtgc ccagcatcag caccagcagc
     4381 agcgcgagga tcaccatgcc gacggtcacc ggcagcgggt cgcgcatacc gatcagcgcg
     4441 atggccgggt agctgagcag caagaagccg atcgccgagg cgagcagcag cggcttgcgc
     4501 ccgatccggt cggacaatgc accgaccggt gcgatcaccg ccatcatccc gagcatcacg
     4561 ccgatgatga tcagcagcga ctcgctctcg ctgatctgca gcacctgcgt cagataggtc
     4621 ggcatgtacg tcagcagcgt gtatacggcg acattgagga ggatcacgaa gccgatcagg
     4681 ttgaggatct gcggccagac ggcctgcatg cactcgcgga acggggcccg cgcggtctcg
     4741 cccgcgttct ccgcgtctcg gaacgccggg gtgtcctcga gccggtaccg caggtagaag
     4801 ccgatgaggc cgagcggccc ggcgatcagg aacgggatgc gccaccccca gctgagcagc
     4861 gcctcctggg gcagcccgag ctggaccgcg gtggcgaggc tcgcgcccag cacgtaaccg
     4921 gcgagcgtgc cgaactcgag aaagctgccg tagaagccgc gcctgtggtc gggcgcgtac
     4981 tcggcgatga acgtggccgc gcccccgtac tcgccgccgg tggagaagcc ctggacgagg
     5041 cgtgcgagca gcagcaggac cggcgccgcg atgccgatcg agtggtagct cgggatgagg
     5101 ccgatacaga acgtggcccc cgacatcatg atgatcgtga aggccagcac ccggcggcgg
     5161 ccgaacttgt cgccgagtgg gccgagcagc accccgccga acggccgcag cacgaacggg
     5221 atcgcgattc cggcgaagac cagcatggtc tgcgctgtgg gatcggcctc cgggaagaag
     5281 ttgagtccga tggccgtgac gaggtagctg tagatcgcgt agtcgaacca ctcgacggcg
     5341 ttgcccatgg ctgcgccggc cacggcgcgc cgcaccgtgc ggtcgtcggc ctgcccgacc
     5401 gactctcgtt tcccttcgac gctcatgatc gacgaccccc attcggcgtt tgcccggcga
     5461 cgctagcccg gaggggtggt cggcgcaggt cggagaaacc gccggggagg tcggtcagga
     5521 gggatcagga ggccagcccg ccgccaacgg cgtcataccc cccgatggac cggaccaggc
     5581 ataggcccag caggactgcc gtccgggcgc cgccgcgagc cgcacccgcc ggtactcgac
     5641 gccctcgtag tcgtcgaggg cgaggagaac ggccgccggg ttgcgtagcg ggaccaccca
     5701 gccgggtgcg cggccgccgg tgccgggcag cagcgcgggg taaccgaacc cggtgtcgag
     5761 caccgtgccg tcgacagcgc cgcgctgtgg ctcaccgtcc gcgtgcgctg cgacccggtg
     5821 ccacgacggg cagcccggtt gcagcagccc gtaggcgaac agcgctgcgg gccattcgtc
     5881 gggatgcggt tcaccgtgca cggtgtcggc gtcgaggccg tcgtcaggtg cggcatcccc
     5941 cgtcagctcg gcggcggcgg cctggggcag gtcgacgcac cgcaccgcgc ggccgtcgac
     6001 gagcagcggg cggcgggtgg ccgcgtgccc gaggtaacac cacggggcgt cgacccgggt
     6061 gccgtcctcg agccgcacct ccccggtgcg cagcctggcc agccggaacc ggtcgtcgcg
     6121 gccctcacag cgatccagca cggcgatctg ctctggagtg gcgagccaca cggcgtggtc
     6181 ctcgacggtg ccggcggagg cggccagcac cgcggggcgc tgtccgtcgc ggtgccgcag
     6241 cccggcggcc cacaccgcgg tcaggtcgcg ggtggtcgca cgtagcacca ccacggggtc
     6301 ggcgcccagc cccagctcgc gccgcaacca ggtgatcttg ctggggcagc ggttggagcc
     6361 gtaggtcagc acggggacgc gcgccgcggc aggtgcggag ccgtgcccgg cgagccaggt
     6421 gtcgagcggg acgtcaccga ggagccatcc tgcccgcggg tcagggcgca gcctgtacga
     6481 gacctcgtcg gtgtgcagga acgaggacgg cgggacggcg ccgggatagg gatcggccgg
     6541 gaagtcggca tcgggccagc tcacgtctcg acggtaacgc gatcaccatg gagccgaaca
     6601 gtgtcgagga aatgaggtcg gcttacccgc ctccggcgcc caggcggcgc ctcggggcag
     6661 gaacctcgtt tccacgacac taggctcggc ccgtgctgcc gcggacgatc gactggttgg
     6721 acggccggat cgcactggtg gaccagacgg ctctgccgga gctgcggatg gtcgagatcg
     6781 ccaccgtgga cgagctcgtc gacgcgatcc agcggctcgt cgtgcggggc gccccggccc
     6841 tcggcgtggc gggtgccctc ggggtcgctc ttgctgccag gaccgtggcc ggtgccgagc
     6901 ttgccgccgt cgctgcccgg ctggccgcgg cgcggccgac ggcggccaac ctcgccgtcg
     6961 gggtgcgtcg cgccatggca gccgcgaccg gcggcccgga ggcggtgctc gccgcggcgc
     7021 tggccgtgct cgacgaggac gtggccgcgt gccgcgcgat cggcaccagg ggtgctgccc
     7081 tgctcgccga gctgtgtggc ccggcgccgc tccggctgca cacgcactgc aacgccggcg
     7141 cactggcctg cgtcgagtgg ggcacggcgc tgggcgtcgt acggtcgttg cacgaggtgg
     7201 gcggggtggc ccatgtcgtc gccgacgaga cccgcccgct cctgcagggc tcgcggatca
     7261 cagccgtcga gctggccgcc atgggcgttc cgttccgcgt ggtggtggac ggcgccggcg
     7321 cgtcgatcat cgctcgcggg ctcgtggacg ccgtcgtcgt cggtgccgac cgggtggccg
     7381 ccaatggcga cgtcgccaac aagatcggca cctaccccct cgcccacgcc gcggcgcggg
     7441 caggcgtccc gttcgtggtg gccgcaccgg agtcgacgct cgacccggac acccccgacg
     7501 gcgacgcgat cgagatcgag gagcgcgcgg cctccgaggt gctcgccttc ggcgggtcgg
     7561 cggcggcacc ggcggggagc gaggtctgga acccggcgtt cgacgtgacc ccggccgacc
     7621 tcgtcacagc gatcgtgacg gaggagcggg tctggcggcc ccgagggtga cgaccacggc
     7681 cggggtatcc ggcttgccat tccggctgat gctcggtgcg accgtgctcg ggttcagcgg
     7741 gtactccctg ctgctgccgg tcgtgccgct ctgggcggcg cgcggaggcg cgggtgcgtt
     7801 gggggctggc gcgacgacag cggtgctcat gctgaccacg gtggtcaccc agctggccgt
     7861 gccgtggctg ctcgtgcgta tcggccaccg ctgggttctc ggcctcggcg tcgcgctgct
     7921 cggtgcgcct gcgccgctgc tcgcactctc ggcccacctc ggcccggtac tggcggtctc
     7981 cgcggtgcgc ggcatcgggt tcggactggc cacggtggcc gggagcgcgt tggtcgccga
     8041 gctcgtaccg cctgcgcagc acgggcgcgc cgtggcccgg tacggcctcg ccgtcgggct
     8101 gccgcagctc gcgctgcttg ccgcgggcgt tgcggtcgtc gagcagctgg gcttcgctgt
     8161 ggtgttcgtc gccgctgggg tgggaccgat gctcggggcg ctcctcgtgc ccgcgatccg
     8221 ggtgccgagg ggcgagggcc gcggcgtccc caccgcggtc
//
LOCUS       DASRSC010000010        14716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2788408,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000010 DASRSC010000000
VERSION     DASRSC010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 14716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14716
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2788408"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..440)
                     /locus_tag="VFE39_00340"
     CDS             complement(147..440)
                     /locus_tag="VFE39_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257077.1"
                     /translation="MSTENTVADRLLQDARRRTAKQESPKLGKVALIVGVLALIISPI
                     SILGWIGGATTLGLGVAAVRRPACAKQAKIAMYLGAAAILVAVFFFSLAIATL"
     gene            709..1617
                     /locus_tag="VFE39_00345"
     CDS             709..1617
                     /locus_tag="VFE39_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257078.1"
                     /translation="MNEHAGPPSAHGEQDDHRSRRMWRALEPVHAVTYFAPESQAACE
                     ALGTKGYWMSYFALRAAPLGAAPPELVAALFYNFHPGLVARAVPAVWELADPERFLAV
                     RLDAVDAALHRLLGEEMLASADMAEAAGIARDAALAAPTAGRALAAANAALDWPDAPH
                     LVLWQAQTVLREHRGDGHVAALLTARLDPVEALVLFAADNAMDVDWLRKRRGWSESEW
                     ADATSRLADRGLLDADGLTTAGRALRAEVEAHTDALAEPPLTAIGPAAADRLVELAAP
                     LVTAIVAADGFLPANPMGLRPLVPTR"
     gene            1787..2701
                     /locus_tag="VFE39_00350"
     CDS             1787..2701
                     /locus_tag="VFE39_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6257079.1"
                     /translation="MAGAVDLSALKARSEVAARPQQASAGAEGAAGSFVIDATEQSFQ
                     ADVLERSLEVPVVVDLWATWCGPCKQLSPVLERLAQAGNGAWILAKVDVDANPRIAQA
                     FGVQSIPMVVAVVGGQPVDAFNGALPEPQVREWIAALLDALRDRMPAIAQAEAGAGAG
                     EAPPEEEPEDPRFTAAEDALERGDYAAAEEAYQQILAAEPANEQAAAALSQVRFLARA
                     ENVDPTAVERADAAPDDVDAQLAAADAEVATDSVDAAFNRLVSTVGRTSGDERDRARE
                     HLIGLFELFPADDSRVTAARRALARALF"
     gene            complement(2769..4139)
                     /locus_tag="VFE39_00355"
     CDS             complement(2769..4139)
                     /locus_tag="VFE39_00355"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HET6257080.1"
                     /translation="MRVPSRHRPRAAAALAVALGVLVTGCAQVVPGRASAAGQQSTAG
                     AAAPSRASGALPLSADETAATATAALQEIWRNDFPAAFARDWTDVAVFAPVDTADPGA
                     PAPPCVARAADLSDQAFYCPAEDAVVWDADGLLPDLHHRFGTAGVVVALAHEMGHAVQ
                     TRLGLDEAQARDPLRYPTILLETMADCYAGAAFARLAERPVAGLTTDLDARDDALLAL
                     VEFRDPLGIEPGNDSAHGNAFDRVSAFQDGFLDGAARCAAMSTDNRVFTQRRFGSAAD
                     RARGGDLPLPELLAAVEQDAAGWFGALAPGWRVPPIAATSCPDHELAPQGPVWFCTSD
                     GVVSVDPAGLTALYREVGDYAVATLVAGRYGVALQGALGADTAGPAAGAAALCLAGAY
                     TGVLVEPDGGFTLSPGDLDEAVQVLLTHDWAARDAEGGVDVREHGFDRVGTFRAGLLG
                     GADTCQ"
     gene            4368..5129
                     /locus_tag="VFE39_00360"
                     /pseudo
     CDS             4368..5129
                     /locus_tag="VFE39_00360"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
     gene            complement(5330..7915)
                     /gene="glgB"
                     /locus_tag="VFE39_00365"
     CDS             complement(5330..7915)
                     /gene="glgB"
                     /locus_tag="VFE39_00365"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET6257081.1"
                     /translation="MNPATEPFAPDPQAVDRVLGGAHHDPHSVLGAHPHPDGMVVRAL
                     RPHADEVHVVREGGEGYPLVKVHDAGLFSGVVPGPPADYRLAVRYGERVDIVDDPYRW
                     LPTLGEIDLHLIGEGRHERLWDVLGAHVRRYDTPAGPVTGTSFAVWAPTAQGVRVTGD
                     FDGWTGWAHPMRVLGSSGVWELFVPGIGAGVRYKFRILGKDGRWRDKADPMAFRTEIP
                     PATASVVDVSTHEWGDEEWMAARGLRQAHAEPMSIYEVHLGSWRQGLTYRELAHELVD
                     YLDEMGFTHVELLPVAEHPFGGSWGYQVTSYYAPTSRFGEPDDFRYFVDHLHRNGYGV
                     IVDWVPAHFPRDEWALAKFDGSPLYEHADPRRGEQPDWGTLVFDFGRTEVRNFLVANA
                     LYWLEEFHIDGLRVDAVASMLYLDYSRPEGQWLPNVHGGRENLDAVAFLQEMNATVYR
                     KHPGVVTIAEESTAWPGVSRPTHLGGLGFGFKWNMGWMHDTLDYIGRDPIYRGYHHNQ
                     MTFSLMYAFSENFVLPLSHDEVVHGKGSLWTRMPGDPWNKSAGLRALLAYMWAHPGKQ
                     LLFQGGEFGQEREWSEQRSLDWHLLEDPLHRGVKTLVGDLNRIYRANPALWTKDTTPE
                     GFSWIDANDASGNVLSFLRHGVDADGKPTVLACVANFSGTPKPDYRVGLPFAGRWREV
                     LNTDAQIYGGSGVGNLGAVEAEQTMWHGRPASVVLQLPPSGVLWLVPEPFAGAARRRA
                     VAAAPADAAPPVTLAGDAVPREEDVVDQAPAAPVTDDAPTEAPPVPEIGDVVAGKPQI
                     VGQEPDEWADGAPTGADFTEPSEITEPSDVAEPSDVAESSEPRAAEPSDGGERNNTER
                     NNTAQ"
     gene            8051..8323
                     /locus_tag="VFE39_00370"
     CDS             8051..8323
                     /locus_tag="VFE39_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HET6257082.1"
                     /translation="MSEVPVRELNQDTAGVLARVKRGEEIDVTERGVVIARLVPAQAH
                     PLADLIAAGVLHPPRRSGPIPRPTGPVSTDSEAGAVLRAMRDDERY"
     gene            8423..8728
                     /locus_tag="VFE39_00375"
     CDS             8423..8728
                     /locus_tag="VFE39_00375"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET6257083.1"
                     /translation="MTSTLSEVELPRALRRIDPTLLAGAPALLERLARQEIDDVVRAT
                     AAAYASAELRFLDAIHLATAQVVIGSRLTAFVTYDRRLLAAATDLSLAVASPGAPTG"
     gene            complement(8742..10139)
                     /locus_tag="VFE39_00380"
     CDS             complement(8742..10139)
                     /locus_tag="VFE39_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HET6257084.1"
                     /translation="MSLAEELPTLLPHWLPQQRWFAAKGRPVREVTVATVTPLLTEGE
                     PLLDLVLVAVTFDDGSPVQHYQILVARREHLRGELEHVVIGQVGSCLAYDGLWDPVVT
                     EWVLDAVRNGLTVGDVRFVPEPGAKIAEGATGRVLGVEQSNTSVSWGDQSILKLFRRV
                     APGLNPDLELHRALRSVGSNEVAALQGAIEGVLDGAPVTLGMLQDFAGNSADGWAMAL
                     ASVRDLLAEGDLRADEVGGDFAAEASRLGETVAVVHAELREALGAGVGDPRALAATWN
                     ERLSTVASEVPALNDYVDPIREVYEAVAALEGSIPTQRVHGDLHLGQTLRTPFGWLVI
                     DFEGEPAAPLAERVKPDSALRDIAGMLRSFDYAAFHQLSKWEPASDWTDPDHDSQMVW
                     RAKEWAERNRSAFCDGYSLRDGADPREQATLLRAFELDKAVYEVLYETRSRPSWAQIP
                     LASIQRLTSETGAHH"
     gene            complement(10136..11884)
                     /gene="treS"
                     /locus_tag="VFE39_00385"
     CDS             complement(10136..11884)
                     /gene="treS"
                     /locus_tag="VFE39_00385"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HET6257085.1"
                     /translation="MSIDPSAAAPLNADDQLPENSSDEYGHARTLDVDRDWFKRAVFY
                     EVLVRAFADSNRDGTGDLRGLTEKLDYLQWLGVDCLWLPPFYDSPLRDGGYDIRDFRA
                     VLPEFGTVEDFVVLLDEAHKRGIRVITDLVMNHTSDTHPWFEESRRNPEGPYGDYYVW
                     SDDDSRYADARIIFVDTETSNWTYDPVRGQFYWHRFFSHQPDLNYDNPAVQEAMIEVL
                     RFWLDLGIDGFRLDAVPYLFEREDTNCENLAESHAFLKRCRKVMDDEYPGRVLLAEAN
                     QWPSDVVEYFGDAEVGGDECHMAFHFPLMPRIFMAVRRENRFPISEILAQTPAIPSGA
                     QWGIFLRNHDELTLEMVTDEERDYMYAEYAKDPRMKANIGIRRRLAPLLENDRNQLEL
                     FTALLLSLPGSPVLYYGDEIGMGDNIWLGDRDAVRSPMQWTPDRNAGFSTCDPGRMYL
                     PIIMDPLYGYQAVNIEAQVNSTTSLLHWNRHMIEVRKRHPAFGLGEYHELGGTNPSVL
                     AYVRSHGDDVVLCVNNMSRFPQPVELDLSQWQGCAPHELTGGVPFPRIGELPYLLTLP
                     GHGFYWFSITPAEESA"
     gene            complement(12275..14245)
                     /locus_tag="VFE39_00390"
     CDS             complement(12275..14245)
                     /locus_tag="VFE39_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HET6257086.1"
                     /translation="MTGRLGIDDISPSVSGGRHPSKAVVGEVVPLGATVWREGHDAVA
                     ANVVWKKVGSRGPAHHVRMTPTGTGTDRFTAVVVADEPGLWTFRVDAWSDPWSTWRHA
                     VTVKLEAGQSAEELANDLEVGARLLQRVGRRPGERGHRDLLFGAANALRDTAMPLGAR
                     VAPALFPAVQTIMTERPVRELVTRGRAQQIYVDRERALFGSWYEFFPRSTGGRDETGR
                     AVHGSFVTAAKQLDRVAAMGFDVVYLPPIHPIGTMHRKGRNNSVNAGPGDVGSPWAIG
                     SAEGGHDAIDPELGTLDDFDAFVARTQDLGMEVALDFALQCAPDHPWVAEHPEWFTVR
                     PDGSIAYAENPPKKYQDIYPINFDNDPAGIYAESLRVVLHWVEHGVRIFRVDNPHTKP
                     PNFWHWLIWQVKARYPDVLFLAEAFTRPARLFGLARLGFTQSYSYFTWRTTKAELTEF
                     ALMHAERADEARPNLFVNTPDILHESLQAGGPGAFAIRATLAATMAPTWGVYSGYELY
                     EWEPAKPGSEEYLNSEKYELRPRDFEGAAASGNSLEPYITRLNEIRRAHPALQQLRDI
                     HFHHVDNDAIIAYSKTDPATGDTVLVVCTLEPHTAQEGNTALDMPALGVDWDDTITVH
                     DEVSGEVYQWGQFNFVRLEPWSHVAHIFRVER"
     gene            14567..>14716
                     /locus_tag="VFE39_00395"
     CDS             14567..>14716
                     /locus_tag="VFE39_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HET6257087.1"
                     /translation="MRALRRFTVRAQLPASLAPLQTLATNLRWSWHPPTQDLFAALDP
                     EAWERA"
BASE COUNT         2097 a         5090 c         5301 g         2228 t
ORIGIN      
        1 tcaaggccga catccggccg ggtcacaccg ggcagctgac ggccaaggtg gagcgggtcg
       61 aggccgcgct cgagaagggc tgacccagcc ccgggtcgag gggttgatgc ccgtggcgcc
      121 agcccctcga cccggagggc cgggcgtcag agcgtggcga tggccaggct gaagaagaag
      181 acggcgacca ggatggccgc cgcaccgaga tacatggcga tcttcgcctg cttcgcgcac
      241 gccgggcggc gcacggcggc gacgccgagg ccgagggtgg tggcgccgcc gatccacccg
      301 aggatcgaga tcgggctgat gatcagggcg agcacgccca cgatcagggc gaccttgccc
      361 agcttcgggc tctcctgctt ggcggtgcgc ctgcgggcgt cctgaagcag gcggtcggcg
      421 acggtgttct ccgtgctcat gcgggttcct tcgagtggga ggccgcgtac cgcggcgagc
      481 gaacttctac gcagagcgac cgttcgatca ctcatacata ctcaacgagt gccatgtccg
      541 cggcggtgtt ctgccagtcc ggtgatcggc tcagccccac accggtcaga tggcggccgt
      601 tgactgcggg cagtgaccgt ttatggccag accgagtgaa tctcgtggtg gtagaccaaa
      661 cgttctactg ttcgggggac cgtcaccgcg ggtgcacgag ggagtgcgat gaacgagcat
      721 gccggcccac catcggccca tggcgaacag gacgatcacc ggtcgcgtcg gatgtggcgg
      781 gcgctcgagc ccgtgcacgc ggtcacctac ttcgcgccgg agtcccaggc ggcatgcgag
      841 gcgctgggca cgaagggcta ctggatgagc tacttcgcgc tgcgggccgc cccgctcgga
      901 gctgcgcccc ccgagctcgt ggcggccctg ttctacaact tccatccggg gctcgtcgcc
      961 agggcggtgc ctgccgtgtg ggagctggcc gaccccgagc gcttcctggc cgtgcgcctc
     1021 gatgcggtcg acgcggcact gcatcggctg ctcggcgagg agatgctcgc ctccgcggat
     1081 atggccgagg ccgccgggat cgccagggac gcggcgctcg ccgcccctac cgctggtcgg
     1141 gccctcgctg ccgccaacgc cgctctggac tggccggacg caccccacct cgtgctctgg
     1201 caggcccaga ccgtgctccg tgagcaccgg ggggacgggc acgtcgccgc gctgctgacg
     1261 gcacgactgg atccggtcga ggcgctggtt ctcttcgcgg ccgacaacgc gatggacgtc
     1321 gactggctgc gcaagcgccg gggctggtcg gagagcgaat gggcggatgc cacgagcagg
     1381 ctcgccgacc gcggcctgct cgacgcggac gggctgacga cggccgggcg cgcccttcgt
     1441 gccgaggtcg aggcccacac cgacgcactc gcggagcctc cgctgaccgc gatcggtccc
     1501 gctgcggccg accggctggt ggagttggcc gcgccgctgg tcacagccat cgtcgccgcc
     1561 gatgggttcc tccccgccaa cccgatgggc ctacgcccgc tcgtgccgac gaggtgagcg
     1621 gcggcagcgc tccgccgcac cccgtcaagt ggccggctgc tcctgcccgc gctcgggagc
     1681 cgggccaccg gtcgaagcgg acgggagacg gcaccggtgg caggatgaac ccgtgtcccg
     1741 accagatcct cgtcaggcgg ccagaacggc cgcgttgtcg tccgccatgg caggtgcagt
     1801 cgacctctcc gccctgaagg cccgctcgga ggtcgccgcc cgaccgcagc aggcgtccgc
     1861 gggagcggag ggtgctgccg gctcgttcgt catcgacgcc accgaacaga gcttccaggc
     1921 cgacgtgctc gagcggtcgc tcgaggtccc ggtcgtggtc gacctgtggg ccacctggtg
     1981 cggcccgtgc aaacagctct cccccgtgct ggagcggctc gcccaggccg gcaacggtgc
     2041 ctggatcctg gccaaggtcg acgtcgacgc caacccgcgg atcgcgcagg cgttcggtgt
     2101 tcagtcgatc cccatggtcg tggccgtcgt cgggggccag ccggtggacg cgttcaacgg
     2161 tgcgctgccg gagccgcagg ttcgcgagtg gatcgccgcg ctgctcgacg cgctgcgcga
     2221 ccggatgccc gccatcgccc aggcggaggc cggtgcaggt gccggggagg caccgccgga
     2281 ggaggagccg gaagacccgc gtttcaccgc ggccgaggac gcgctcgagc gcggtgacta
     2341 tgcggctgcg gaggaggcct accagcagat cctcgccgcc gagccggcga acgaacaggc
     2401 cgcagcggcg ttgtcgcagg tgcggttcct ggccagggcg gagaacgtcg acccgacggc
     2461 cgtcgagcgc gcggacgccg cgcccgacga cgtcgacgca caactcgccg cggcggacgc
     2521 cgaggtggcg acggacagcg tcgacgcggc gttcaaccgg ctcgtgtcca cggtcgggcg
     2581 cacatcgggc gacgagcgcg accgcgcccg cgagcacctc atcgggctgt tcgagctgtt
     2641 cccggccgac gactcccgcg tcaccgcggc ccggcgagca ctcgcccgcg cgctgttctg
     2701 agctctgcct gtcaggcggc ggttcgtgcg cggatacggg tgctccagca ctcgtatccg
     2761 cgcacgggtc attgacaggt gtccgccccg ccgagcagcc cggcgcggaa cgtgccgacg
     2821 cggtcgaacc cgtgctcacg gacgtcgacg ccgccctcgg cgtcccgcgc ggcccagtcg
     2881 tgggtcagca gtacctgtac ggcttcgtcg aggtcgccgg gggagagcgt gaacccaccg
     2941 tcgggctcga ccagcacgcc cgtgtaagcc cctgccaggc acagtgcggc tgcaccggcc
     3001 gccggcccag cggtgtcggc gccgagcgcg ccctgtaggg ctacgccgta gcggccggcc
     3061 acgagggtgg ctacggcgta gtcgccgacc tcgcggtaga gggcggtgag gccggcgggg
     3121 tcgaccgaca ccactccgtc ggacgtgcag aaccacaccg gcccttgggg cgccagctcg
     3181 tggtccgggc agctcgtcgc cgcgatcggc gggacccgcc agccgggcgc cagcgcgccg
     3241 aaccatccgg cggcgtcctg ctccacagcg gcgagcagct ccggcagcgg gaggtccccg
     3301 ccacgggcgc ggtcggccgc cgagccgaac cgccgctgcg tgaacacgcg gttgtccgtg
     3361 ctcatcgcgg cgcagcgggc ggccccgtcc aggaagccgt cctggaacgc cgagacgcgg
     3421 tcgaacgcgt tgccgtgagc gctgtcgttg ccgggctcga tgccgagcgg gtcgcggaac
     3481 tcgacgagcg ccagcagcgc gtcgtcgcgc gcatcgaggt cggtggtgag cccggcgacg
     3541 ggccgctcgg ccaggcgcgc gaaagcggca cccgcgtaac agtcggccat cgtctcgagc
     3601 aggatcgtcg gatagcgcag tgggtcgcgg gcctgcgcct cgtcgagccc gaggcgggtc
     3661 tgcaccgcat ggcccatctc atgggccagc gcgaccacga cccctgcggt gccgaaccga
     3721 tggtgcagat cgggcaggag gccgtctgcg tcccagacga cggcatcctc ggcggggcag
     3781 tagaacgcct ggtcggagag gtcggcggcg cgggcgacgc agggcggcgc gggtgcgccc
     3841 ggatcggcgg tgtcgaccgg cgcgaagact gcgacgtcgg tccagtcccg ggcgaacgcg
     3901 gcggggaagt cgttgcgcca gatctcctgc agggctgcgg tggccgtggc cgcggtctcg
     3961 tcggcggaca gcgggagtgc tccggatgcg cgactgggcg ccgcggcgcc ggcggtggac
     4021 tgttgccctg ccgcgctcgc ccggccaggg acgacctgcg cacaccccgt gaccaggacg
     4081 cccagcgcga cggcgagcgc ggctgcggcc cggggccggt gccgggacgg gacacgcacg
     4141 ggagaatcat cgcccgtcgc cacgctccgg gggagcggtt cggccgaaca cgaccgggcg
     4201 ggatagcgtc aggcaccgtg tcgttgatga gggacctcca cgccggccgg accgggattg
     4261 tgcggtgggg aatcgccggg ctactcgtgg tggcgtcgat cgtcacgacg gtcgtgctgt
     4321 acgtgctgca tcccgacgcg ctggtgacct tgctgccttc gaccgacatg cgtgagctgc
     4381 acgtcgactt cgacacgttc tggcactcgg ccgtcgcgct gacccagggc gccgacatct
     4441 accacacctc tgccaagctc acgaacctca acccgccgct gctcacggtg ctgctcacgc
     4501 cactcacgct gttcgactcg ctcaccgcgt accgggtctt cgcggtgctc acgctgctga
     4561 tcgtcgtggg cgcggtactg gccgtggccc gcgagctgcg cctgcaccgg gctgccacgg
     4621 cgctggccgt gctggccgtg ctggcgtcat cgccgctgca cggcaccctc gtgctcggcc
     4681 agatctaccc gttgctgctc gccggcctgg tcgcgggctg gattgcggaa cggcgcggcc
     4741 ggccggtgct cgccgccgtg ctcttcgggg tcacggtggc gctcaagccg tcgctggccc
     4801 cgctgctgct gctccccgcg gtgcagcggc ggtgggtctc gctgtggacc gggctgggtg
     4861 ccgcgacggc cgccactgtc gtcggggtac tcgtcgccgg gccgtcgagc gggttcgagt
     4921 ggctccacat cgcggtcacc gagcccgtgc cggacaccgt cgacaacgcc tcgctgcccg
     4981 ggctcgccgt gcggttcggc gtgccgtcgg tactgggcac gttgctcggg gcggtcgtgc
     5041 tgatcagcac actggtgtgg tgcgggcgcc accggcaccg catcgacccg gccggtgctg
     5101 ccccgtgggc cgtgctcgcc gcgggactgc tcgtctcccc gatctcgtgg cacaactacc
     5161 tgatgctgct ctggccgggt gtgctcgtgc tgatcacgct cgggcgtggc gcggccgctg
     5221 cagtcgcgct cgcggtgatc gtgataccgg tgtcctggaa cgcgggctgg ccgcccgagg
     5281 gcttcgggcc ggacgtcgcg cggtcgctct actgcgccat cctgatcggc tactgggcgg
     5341 tgttgttgcg ctcggtgttg ttgcgctcgc cgccgtccga aggctcggct gccctcggct
     5401 ccgacgactc ggcgacgtcc gacggctcgg cgacgtccga cggctcggtg atctccgacg
     5461 gctcggtgaa gtccgcgccg gtcggtgcgc cgtccgccca ctcgtccggc tcctgaccca
     5521 cgatctgggg cttcccggcc acgacgtccc cgatctccgg cacgggcggt gcctccgtgg
     5581 gggcgtcgtc ggtgacggga gccgccgggg cctggtcgac cacgtcctcc tcccggggga
     5641 cggcgtcgcc ggcgagggtg acgggcggtg ccgcgtcggc cggggccgcg gcgaccgcac
     5701 gcctgcgggc cgcacccgcg aacggctcgg gaaccagcca caacacaccc gagggcggca
     5761 gctgcagcac cactgacgcc ggcctcccgt gccacatcgt ctgctcggcc tccaccgcgc
     5821 cgaggttgcc caccccggag ccgccataga tctgcgcgtc ggtgttgagc acttcgcgcc
     5881 agcgcccagc gaagggcaag ccgacccggt agtccggctt cggggtaccc gagaagttcg
     5941 ccacgcaggc gagcaccgtt ggcttgccgt cggcgtcgac cccgtgccgc aggaagctca
     6001 ggacgttgcc ggaggcgtcg ttggcgtcga tccaggagaa cccttcaggg gtggtgtcct
     6061 tcgtccacag ggcggggttg gcgcggtaga tccggttgag gtcgccgacg agcgtcttga
     6121 cgccgcggtg cagggggtcc tccagcaggt gccagtccag cgaccgctgc tcggaccact
     6181 cccgctcctg gccgaactcg ccgccctgga acaacagctg cttgcccggg tgggcccaca
     6241 tgtaggccag aagcgcgcgc agtcctgccg acttgttcca cgggtcgccg ggcatccggg
     6301 tccacagcga gcccttgccg tgcaccacct cgtcgtgcga cagcggcagc acgaaattct
     6361 cgctgaacgc gtacatgagc gagaacgtca tctggttgtg gtggtagccc cggtagatgg
     6421 ggtcgcgccc gatgtagtcg agcgtgtcgt gcatccagcc catgttccac ttgaagccga
     6481 atcccaggcc accgaggtgg gtggggcggg agacgcccgg ccaggccgtg gactcctcgg
     6541 cgatcgtgac gacgcccggg tgtttgcggt acacggtcgc gttcatctcc tgcaggaacg
     6601 cgacggcgtc gaggttctcc cggccaccgt gcacgttggg cagccactga ccctcggggc
     6661 gggagtagtc gaggtagagc atgctggcca cggcgtccac ccgcaggccg tcgatgtgga
     6721 actcctccag ccagtagagc gcgttcgcga cgaggaagtt gcgcacctcc gtgcgcccga
     6781 agtcgaacac gagcgtgccc cagtcgggct gctcgccgcg gcgcgggtcg gcgtgctcgt
     6841 agagcgggga tccgtcgaac ttcgccagcg cccactcgtc acgcgggaag tgcgccggca
     6901 cccagtcgac gatcacgccg tagccgttgc ggtgcaggtg gtcgacgaag taccggaagt
     6961 cgtcgggttc gccgaaccgc gatgtcggcg cgtagtacga cgtcacctgg tagccccacg
     7021 acccgccgaa cgggtgctcg gccacgggta gcagctcgac gtgggtgaag cccatctcgt
     7081 cgaggtagtc gaccagctcg tgcgccagct cccggtaggt gaggccctgg cgccacgaac
     7141 cgaggtgcac ctcgtagatg ctcatcggct cggcgtgcgc ctggcgcagc ccccgagccg
     7201 ccatccactc ctcgtcgccc cactcgtggg tcgagacgtc gacgaccgac gccgtggccg
     7261 gcgggatctc ggtacggaac gccatcgggt cggccttgtc gcgccaccgg ccgtccttgc
     7321 cgaggatgcg gaacttgtag cggacgcccg cgccgatgcc cgggacgaac agctcccaca
     7381 ccccggagct gcccagcacg cgcatcgggt gtgcccagcc cgtccagccg tcgaagtcgc
     7441 cggtcacccg tacgccttgc gcggtgggtg cccagacggc gaagctcgtg cccgtgacgg
     7501 ggccggcggg cgtgtcgtag cggcgcacgt gtgcgccgag cacgtcccac agccgctcgt
     7561 ggcggccctc tccgatcagg tgcaggtcga tctcgcccaa cgtgggcagc cagcggtacg
     7621 ggtcgtcgac gatgtccacg cgctcgccgt accggaccgc aagccggtag tccgcgggcg
     7681 ggccgggcac gacgccggag aacagcccgg cgtcgtgcac cttcacgagg ggatacccct
     7741 cgccgccctc acggaccacg tgcacctcgt cggcgtgcgg gcgcagtgcc cggacgacca
     7801 tgccgtccgg gtgcgggtgc gcgccgagca cggagtgcgg gtcgtggtgc gctcccccga
     7861 gcacgcggtc gaccgcctgc gggtccggtg cgaacggctc cgtcgcaggg ttgatcgtct
     7921 gggtggggtt ctcctcgcga tccactcgcg caccctattg ccaacaggtg ctcgtcgccg
     7981 accctgggca cgcggacgga cgacggcgaa cgcacgtagg tgtactacgg tttcctagta
     8041 ccctgctgcc gtgagtgagg ttccggtccg cgagctgaac caggacacgg caggcgtgct
     8101 ggctcgggtc aagcggggcg aggagatcga cgtgaccgag cggggggtcg tcatcgcgcg
     8161 gctcgttccg gcccaggctc acccgcttgc tgacctgatc gccgccgggg tactgcatcc
     8221 gcccaggcgg agtggcccga tccctcgtcc gaccggtccc gtgagcaccg acagcgaggc
     8281 cggggccgtc ctccgcgcga tgcgtgacga cgaacggtac tgatgatcta cctcgacacg
     8341 gctgcgctgg tgaactgctt cgtcgcgaac gggagaggac gcatttgtcg actggctcga
     8401 cgacaggccg gacacgctcc tcgtgacgtc gacactctcc gaggtggagt tgcctcgcgc
     8461 actgaggcgc atcgacccga cgctgctggc aggcgcgcct gcgctgctcg aacggctggc
     8521 gcggcaggag atcgacgacg tcgtgcgtgc gaccgccgcc gcgtacgcga gcgcggagct
     8581 gcgcttcctc gacgccatcc acctcgccac cgcacaggtc gtcatcggct cgcgcctcac
     8641 ggcgttcgtg acctacgacc ggcgcctgct cgctgccgcg accgatctgt cgctcgcggt
     8701 ggccagcccg ggcgcgccaa cggggtgagg cttgcgctga atcagtggtg tgcgcccgtc
     8761 tcgctggtca ggcgctggat cgaggcgagc gggatctgtg cccacgacgg acggctgcgg
     8821 gtctcgtaca gcacctcgta cacggccttg tcgagctcga acgcccggag cagggtggcc
     8881 tgctcccgcg ggtcggcgcc gtcgcggagc gaatagccgt cgcagaaggc ggaccggttg
     8941 cgctcggccc actccttcgc gcgccacacc atctgggagt cgtggtccgg gtcggtccag
     9001 tcgctcgcag gctcccactt ggacagctgg tggaacgccg catagtcgaa cgagcgcaac
     9061 atcccggcga tgtcacgcag cgccgaatcg ggcttgacgc gctccgcgag cggcgcagcg
     9121 ggctctccct cgaagtcgat caccagccag ccgaaggggg tgcggagcgt ctggccgagg
     9181 tgcaggtcgc cgtgcacgcg ctgggtgggg atcgagccct cgagtgcggc gacggcctcg
     9241 tagacctccc ggatcgggtc cacgtagtcg ttcagcgcgg gcacctcact ggccaccgtg
     9301 gagagccgct cgttccacgt ggccgccagc gccctggggt cgcccacgcc cgcgccgagg
     9361 gcctcgcgca gctcggcgtg caccaccgcg accgtctcgc ccaaccgcga cgcctccgcc
     9421 gcgaagtcgc cacccacctc gtcggcgcgc agatcgccct cggccagcag gtcgcgcacg
     9481 ctcgccagtg ccatcgccca gccatccgcc gagttgcctg cgaagtcctg cagcatcccg
     9541 agcgtgaccg gcgcgccgtc cagtacgccc tcgatggcac cctgcagggc cgcgacctcg
     9601 ttgctgccca ccgagcgcag cgcgcggtgc agctccagat cggggttcag gccgggggcc
     9661 acccgtcgga acagcttgag gatcgactga tcgccccacg acacggacgt gttggactgc
     9721 tcgacaccga gaacacggcc ggtggcgcct tccgcgatct tcgcaccggg ctcgggaacg
     9781 aaccggacgt cgccgacggt gagcccgttg cgcacggcgt ccaggaccca ctcggtgacc
     9841 accggatccc acagcccgtc gtatgcgaga cagctaccga cctgtccgat cacgacgtgc
     9901 tccagctcgc cgcgcaggtg ctccctgcgg gcgacgagga tctggtaatg ctgcaccggg
     9961 gagccgtcgt cgaatgtgac ggcgaccaga acgagatcga gaagcggttc accctcggtc
    10021 agcagcggtg tcacggtggc caccgtgacc tcgcggaccg gacggccctt ggcggcgaac
    10081 cagcgttgct ggggcagcca gtggggcagc agggttggaa gctcctcggc cagactcacg
    10141 cagactcctc cgcaggggtg atcgagaacc aatagaagcc atgccccggc agcgtcagca
    10201 ggtagggcag ctcaccgatt cgcgggaagg gcactcctcc ggtgagttca tggggtgcac
    10261 aaccctgcca ctgcgacagg tcgagctcca cgggctgcgg gaacctcgac atgttgttga
    10321 cgcacagcac gacgtcgtcg ccgtggctgc gcacgtatgc cagcacggac gggttcgtac
    10381 cgccgagctc gtggtactcg cccaggccga acgcggggtg gcgcttgcgg acctcgatca
    10441 tgtgccggtt ccagtgcagc agtgacgtcg tggagttgac ctgcgcctcg atgttcacgg
    10501 cctgatagcc gtagagcggg tccatgatga tcggcaagta catccggccc gggtcgcagg
    10561 tggagaaccc ggcattgcgg tcgggcgtcc actgcatcgg gctacgcact gcgtcgcggt
    10621 cgccgagcca gatgttgtct cccatgccga tctcgtcacc gtagtacaga accggtgaac
    10681 ctggcagcga gagcagcagc gcggtgaaca gttccagctg gttgcggtcg ttctccagca
    10741 ggggggccag ccggcggcgg atcccgatgt tggccttcat ccgcgggtcc ttggcgtact
    10801 ccgcgtacat gtagtcgcgc tcttcgtcgg tgaccatctc gagcgtcagc tcgtcgtggt
    10861 tgcgcaggaa gatgccccac tgcgcgcccg acgggatcgc cggcgtctgg gccaggatct
    10921 ccgagatcgg gaagcggttc tcgcggcgca ccgccatgaa gatgcgcggc atcagcggga
    10981 agtggaaggc catgtggcac tcgtcgccgc cgacctcggc gtcgccgaag tactccacga
    11041 cgtccgacgg ccactggttg gcctccgcga ggagcacccg gccggggtac tcgtcgtcca
    11101 tcaccttccg gcaccgcttg aggaaggcgt gcgactcggc gaggttctcg cagttggtgt
    11161 cctcgcgctc gaacaggtac ggcacggcat cgagccggaa accgtcgatg cccaggtcga
    11221 gccagaagcg caggacctcg atcatcgctt cctgcactgc cgggttgtcg tagttcaggt
    11281 cgggctggtg ggagaagaag cggtgccagt agaactggcc gcgcaccggg tcgtaggtcc
    11341 agttcgacgt ctcggtgtcg acgaagatga tccgtgcgtc ggcgtagcgg gagtcgtcgt
    11401 cggaccagac gtagtagtcg ccgtagggac cctcggggtt gcggcgggac tcctcgaacc
    11461 acgggtgggt gtcggaggtg tggttcatga ccaggtccgt gatgacccgg ataccccgct
    11521 tgtgtgcctc gtcgagcagg actacgaagt cctcgaccgt gccgaactcg ggcagcacgg
    11581 cccggaagtc gcggatgtcg tagccaccgt cgcgcagcgg ggagtcgtag aagggcggca
    11641 gccagaggca gtcgacgccg agccactgca ggtagtcgag cttctcggtc agaccgcgaa
    11701 ggtcgccggt gccgtcgcgg ttggagtcgg caaacgctct gaccagcacc tcgtaaaaca
    11761 cggcccgttt gaaccagtcg cgatcgacat ccagtgtgcg cgcatggccg tactcgtcgg
    11821 aggagttctc gggcagctgg tcgtcggcgt tgagcggggc ggcggccgag gggtcgatcg
    11881 acataggggt atcgcgttcc taccgggtgg ggatggatgg cggaaggggc accccaccag
    11941 tcttcccggg cgattgcccg agcgcatgac gtggctcgag gagctgcccc gcagcgtagc
    12001 cgccgttctc gtccggacca cgacagctcg gccgcggtga gacacgcatc gcacgaaaga
    12061 gggagaggat tcactacccg gagccgacag ccgatggcgt cgtccagtgc acgggcccgg
    12121 ccgctccggc cggcggagca ggtgggagag cacggtgccg ccccgcaagc ggtgagcagg
    12181 gcacagccag cctgcggccg cagtacagat gtcggtgagc agaacctgct cggatgcctc
    12241 gcggcgctcg cgcgtcaggg cgccgctttc cgccctaccg ctccacccgg aagatgtgcg
    12301 ccacgtggct ccagggctcc agccggacga agttgaactg gccccactgg tagacctcgc
    12361 cgctcacctc gtcgtgcacg gtgatggtgt cgtcccagtc cacgccgagc gccggcatgt
    12421 cgagcgcggt gttgccctcc tgtgcggtgt gcggttcgag cgtgcacacc accaggaccg
    12481 tgtcgcccgt ggccgggtcg gtcttcgagt aggcgatgat cgcgtcgttg tccacgtggt
    12541 ggaagtggat gtcgcgcagt tgctgcagcg ccgggtgcgc gcgccggatc tcgttgagcc
    12601 gcgtgatgta gggctcgagc gagttgccgc tggccgcggc gccctcgaag tcgcgcggac
    12661 gcagctcgta cttctcggag ttgaggtact cctcgctgcc cggcttggcg ggctcccact
    12721 cgtacagctc gtagcccgag tagacgcccc acgtgggcgc catcgtggcg gcgagggtgg
    12781 cccggatggc gaacgctccc gggccgccgg cctgaagcga ctcgtggagg atgtccggcg
    12841 tgttgacgaa caggttgggc ctggcctcgt cggcgcgttc ggcgtgcatc agcgcgaact
    12901 cggtgagctc cgccttcgtg gtgcgccagg tgaagtagct gtaggactgg gtgaacccga
    12961 gccgggccag cccgaacagg cgggccgggc gggtgaacgc ctcggccagg aacagcacgt
    13021 ccggataccg ggctttcacc tgccagatca gccagtgcca gaagttcggc ggcttggtgt
    13081 gggggttgtc gacgcggaag atccggacgc cgtgctcgac ccagtgcagt accacgcgga
    13141 gcgactcggc gtagatcccg gccgggtcgt tgtcgaagtt gatgggatag atgtcctggt
    13201 acttcttcgg cgggttctcg gcgtaggcga tggagccgtc ggggcggacg gtgaaccact
    13261 ccggatgctc ggccacccac gggtggtcgg gcgcgcattg caatgcgaaa tcgagcgcca
    13321 cctccatgcc gagatcctgc gtgcgcgcaa cgaacgcgtc gaagtcgtcg agggtgccga
    13381 gttcggggtc gatcgcgtcg tgaccgccct cggcggagcc gatcgcccac ggcgagccga
    13441 catcgccggg acccgcattc accgagttgt tgcgcccctt gcggtgcatc gtgccgatcg
    13501 gatggatcgg cgggagataa acgacgtcga agcccatcgc cgcgacccgg tcgagctgct
    13561 tggctgccgt gacgaacgac ccgtgcactg cgcggccggt ttcgtcgcgg ccgcccgtgg
    13621 agcgggggaa gaactcgtac caggagccga agagcgcgcg ctcgcggtcg acgtagatct
    13681 gctgggcgcg gccgcgggtg accagctccc ggaccgggcg ctcggtcatg atcgtctgca
    13741 cggcggggaa cagcgccggc gcgacgcggg cgcccagcgg catcgccgtg tcgcgcaggg
    13801 cgttggcggc gccgaagagc aggtcgcgat gcccgcgctc accgggccga cggccgaccc
    13861 gctggagcag ccgggccccg acctcgaggt cgttggccaa ctcctcggcg ctctgcccgg
    13921 cttcgagctt gacggtgacc gcgtgtcgcc aggtggacca cgggtcgctc caggcgtcga
    13981 cccggaaggt ccacaggccc ggctcgtcgg cgaccacgac ggcggtgaac cggtcggtgc
    14041 cggtgccggt gggcgtcatg cggacatggt gggcggggcc gcggctgccg accttcttcc
    14101 agaccacgtt ggcggcgacg gcgtcgtggc cctcgcgcca caccgtggcg ccgagcggga
    14161 ccacctcgcc cacgacggcc ttgctgggat ggcggccgcc actgaccgag gggctgatgt
    14221 catcgattcc cagccgaccg gtcatcggct cacccccagc tgcatgcccg tcgcggaagt
    14281 cacaaaccta tctacccgtt gtgcgcctga tcagaacggg ccgccgccgt tcacgtgatc
    14341 gtgaccagat gtccgccgtc cggatgtaaa ccagggcccg cgccgccccg aaggagtaca
    14401 ggcagtggca tcgaggaggc tgcggcaccc gctggccgct gatcaaggcg cagacgcggt
    14461 tcgcggagat cattgaacga cacctgtgtc ttgatcgcca ggggagcagg gatatgggct
    14521 cctccacttg accgatcacg ggatccattc atctatggtc cccgccgtga gagctctccg
    14581 gcggttcacc gttcgtgctc agctgcctgc gtccctggca ccgctgcaga cgctggccac
    14641 caacctgcgc tggagctggc atcccccgac gcaggacctg ttcgcagccc tcgaccctga
    14701 ggcatgggaa cgggcc
//
LOCUS       DASRSC010000011         1646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4262071,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000011 DASRSC010000000
VERSION     DASRSC010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4262071"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VFE39_00400"
     CDS             <1..1129
                     /locus_tag="VFE39_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6257088.1"
                     /translation="TLVGMFRDIELAEDVAQEAFAIAAERWPRDGEPANPTAWLITTA
                     RNRAIDQIRRQQVLAEKTRLIAHELTAHLEPTMDEPTTFRDERLELIFTCCHPALSRE
                     AQVAMTLRTLGGLSTEEIARAFLTPFDTMSKRLTRAKHKIRDARIPFAVPEDHQLPDR
                     LAAVLVIVYLVFNQGWSDGRVDLAAEAIRLGRALAELMPDESEALGLLALMLLNDARR
                     EARVREGVLVPLDEQDRSLWDEQQILEGRELLERAVARQSAGSYVIQAAIADLHLQQP
                     RDWQQIAALYGTLAKRTGSPVVELNRAVALAELDGPDAGLALLDTLTLDGYRYYHSTR
                     AELLRRAGRTGDARAAYARALELAETDPERQFLARRLEELT"
     gene            complement(1197..1361)
                     /locus_tag="VFE39_00405"
     CDS             complement(1197..1361)
                     /locus_tag="VFE39_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257089.1"
                     /translation="MRTTSRPAPIPRRDVDLASYGLVSMLPQGPGSGYQGLGWRPRLA
                     FEAMAELKAG"
BASE COUNT          255 a          593 c          553 g          245 t
ORIGIN      
        1 gacgctggtc gggatgttcc gggacatcga gctggccgag gacgtggccc aggaggcctt
       61 cgcgatcgcg gcagagcgct ggccccggga cggcgagccc gccaacccga ccgcctggct
      121 gatcacgacg gcacggaacc gggcgatcga ccagatccgc cggcagcagg tgctcgccga
      181 gaagacccgg ttgatcgccc acgagctcac tgcgcacctg gaacccacga tggacgaacc
      241 gacgaccttc cgggacgagc gtctcgagct gatcttcacc tgctgccacc cggcgctgtc
      301 ccgtgaggcg caggtggcga tgaccctccg gaccttgggc ggcctgtcga ccgaggaaat
      361 cgcgcgcgcg tttctgaccc cgttcgacac gatgagcaag cgcctcaccc gcgccaagca
      421 caagatccgc gacgcgcgca tcccgttcgc cgtgccggaa gaccaccagt tgcccgaccg
      481 gctcgcggcc gtcctcgtga tcgtctacct cgtcttcaac cagggctgga gcgatggtcg
      541 ggtcgacctg gccgccgagg cgatccgact gggccgggcg ttggccgagc tcatgcccga
      601 tgagtcggag gccctcgggc tgttggccct gatgctgctg aacgacgccc gccgcgaagc
      661 ccgagtgcgc gagggcgtgc tcgtcccgct ggacgagcag gaccggtcgt tgtgggacga
      721 gcagcagatc ctggagggtc gcgagcttct cgagcgggcg gtcgcccggc agagcgccgg
      781 gagctacgtc atccaggcag ccatcgccga cctgcacctg cagcaacccc gtgactggca
      841 gcagatcgcc gcgctctacg ggacgctcgc caagcgcacc ggatcaccgg tggtagagct
      901 caaccgcgcc gtggccctcg cagagttgga cggcccggac gcgggtcttg ccctcctcga
      961 caccctcacc ctcgacggct accgctacta ccactcgacc cgggccgagc tcctccgtcg
     1021 cgccgggcga acgggcgacg ctcgggctgc gtatgcgcgc gccctcgagc tcgccgagac
     1081 cgaccccgag cgtcagttcc tcgcgcgtcg actcgaggaa ctgacctgac cgatcgcact
     1141 ggaacggcgt gacgcgccgc gctctgccct gcggacccgg gggctggccg acgctgtcag
     1201 cccgccttga gctcggccat agcctcaaag gccagcctgg gccgccagcc caggccctga
     1261 tagccgctgc ccggtccctg cggcagcatc gacaccaggc cgtagctggc caggtcgacg
     1321 tcccggcggg ggatcggtgc tggtcggctg gtggtacgca tagcttgctg tatttcgctt
     1381 ctgtacggag gggcagacgc tacaggaaca agccttgatc tccacagggt ctccacaagt
     1441 cctgcacccg gcctccgcac gccatgcatc tgctggtgcc cggcccgcga cgcgtgagcg
     1501 tcgagagcga gaggtacgac gatgttcctg gatctcctag cgggcggcgg gctcctcgct
     1561 gccggttata tcgtgggcta cctcaccgcg cggaccgttc atcggcgctc ggcagggcag
     1621 gaagactgca cttgcgggca ccccgt
//
LOCUS       DASRSC010000012         4837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3606939,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000012 DASRSC010000000
VERSION     DASRSC010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3606939"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="VFE39_00410"
     CDS             <1..589
                     /locus_tag="VFE39_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6257090.1"
                     /translation="ADLLADSAAAVLALDYDPVTAAHVARRYPRVGSARANLVALPVR
                     DGRCDAVVSLQVIEHLWEQERFLRECHRVLRPGAALLLSTPNRLTFSPGRDTPLNPFH
                     TQELSGTELAGLVRGAGFVDVEILGLHHGRRLRELDARHGGSIVDAQVAVAVAGAQWP
                     PALLGDVASVTCDDFDLTAGDLDASLDLVALAVRP"
     gene            586..2058
                     /locus_tag="VFE39_00415"
     CDS             586..2058
                     /locus_tag="VFE39_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HET6257091.1"
                     /translation="MSEPAGTFCLVLHSHLPWLAHHGRWPVGEEWLYQSWAHAYLPVL
                     DLVERLAADGYRDLLTLGVTPVLAAQLDDPHCLRGMHDWLGGWLLRAHGAASRLPDLA
                     SHEHRAATAALDLFESRWRHGASPMLRRLADAGAVELLGGPATHPFAPLLLPEVRAFS
                     LETGLADAALRLGSRPAGIWAPECGYAPGMEHGYAAAGVRRFLVDGPALRGDTALARP
                     VGTSDVLAFGRDLQVTYRVWSPRSGYPGGRDYRDFHTFDHPSGLKPARVTGRSVAPED
                     KRPYEPARAAAAVARDAADFVAVVRDRLLRLRERLGRPALTVAAFDTELFGHWWHEGP
                     AWLEAVLRALPAAGVRVTTLRGAVDAGLVGEPVELPPSSWGSGKDWRVWAGEQVADLV
                     ALNEQVQKTLSGAVAPDVARDPLRDALAQEALLALSSDWAFMVSKDSAAGYARDRAHG
                     HAGRVEELARLLAADRRPAAARRVASWPPAPFGHVDARTL"
     gene            2449..3702
                     /locus_tag="VFE39_00420"
     CDS             2449..3702
                     /locus_tag="VFE39_00420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6257092.1"
                     /translation="MRVLMVSWEYPPVVIGGLGRHVHALAEELAAAGHEVVVLSRQPA
                     GTDAATHPTLDELVGGVRVLRVAEDPAHLEFEHDMVAWTLAMGHALLRATLTRLTDWR
                     PDVVHAHDWLVAHPAIALADVLGVPLVATIHATEAGRYAGWLSAPLSRQVHSVEWWLA
                     NRADAVITCSSAMRDEVAELFELDPGRIAVLHNGIVPRRWRTSRARVTSARNRFAPGG
                     GPLLLYFGRLEYEKGVQDLIAALPSIRRAHPGTRALVAGTGTAAEMLVESARRHRVRR
                     SVDFLGHLPDEDLVALLAAADAVVLPSRYEPFGIVALEAAAAGAPLVASTAGGLGEVV
                     VDGETGMSFAPGDVEALARAVGSVLTDPKAAERRADAARTRLAVDFDWARITAGTAAV
                     YAATHAGGAIESGRPKIPTGNVFGR"
     gene            complement(3719..3877)
                     /locus_tag="VFE39_00425"
     CDS             complement(3719..3877)
                     /locus_tag="VFE39_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257093.1"
                     /translation="MKDDTVRRLNALDDEYTAAVNAAVAEDRDDLIQQLVSEYPDAAL
                     EIMNDEAA"
     gene            complement(3986..4762)
                     /locus_tag="VFE39_00430"
     CDS             complement(3986..4762)
                     /locus_tag="VFE39_00430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET6257094.1"
                     /translation="MTSMWGAPLRDRWGRAFRRDPRQARFLTIASLRWVLRNRAYTPW
                     YLVRYWRLLRFRLANPHVILRGMVFLGRRVEVHARPGYGRLEIGRWVHIGDGNSLRCH
                     EGSLRIGDKVVFGKDNTVNCYLDVEIGASTIVADWVYVTDFDHRIEDVRVPIKDQGIV
                     KTPVRIGPDCWIGVKVSVLRGTRVGRGSVLGAHAVVRGDIPEYSVAVGAPARVVRNRL
                     ADFDAAADRRAALADIARKTEAAVRERVEQTSGSRIPKES"
BASE COUNT          601 a         1770 c         1745 g          721 t
ORIGIN      
        1 cgccgacctg ctggccgaca gcgcagccgc cgtgctggcg ctggactacg accccgtcac
       61 cgccgcgcac gtggcacggc gctatccgcg cgtcgggtct gcacgtgcca acctcgtggc
      121 actccctgtg cgggacggcc gctgcgacgc ggtggtctcg ctgcaggtga tcgagcacct
      181 ctgggagcag gagcggttcc tgcgcgagtg ccaccgggtg ctgcgccccg gcgcagcgct
      241 gctgctctcc acgcccaacc gcctgacgtt ctcccccggc cgcgacaccc cgctcaaccc
      301 gttccatacc caagagctct cgggaaccga gctggcgggg ctggtccggg gcgccggatt
      361 cgtcgacgtc gagatcctcg ggttgcacca cggcaggcgg ctgcgcgagc tcgacgcccg
      421 gcacggcggc tcgatcgtcg acgcgcaggt cgccgtggcc gtcgcgggag cgcagtggcc
      481 gccggccctg ctgggcgacg tcgcctcggt gacgtgtgac gacttcgatc tcaccgcggg
      541 cgacctcgac gccagcctgg atctcgtcgc cctcgccgtg cgcccgtgag cgagcccgcc
      601 ggaacgttct gcctggtcct gcacagccac ctgccgtggc tcgcacacca cggacgctgg
      661 ccggtcggcg aggagtggct ctaccagtcg tgggcacacg cctacctccc ggtgctcgac
      721 ctcgtggagc gcctcgccgc cgacgggtac cgcgacctgc tgacgctcgg cgtcacgccg
      781 gtgctcgccg cgcagctcga cgacccgcac tgcctgcgcg gcatgcacga ctggctcggc
      841 ggctggctgc tgcgcgccca cggcgccgca tcccggctcc cggacctcgc atcgcacgaa
      901 catcgcgccg cgaccgctgc gctcgacctg ttcgagtcac gctggcgaca cggcgcctcc
      961 ccgatgctgc gccgcctcgc cgacgcgggg gcggtcgagc tgctcggtgg gcccgcgacg
     1021 cacccgttcg ccccgctgct gctccccgag gtccgtgcgt tctcactcga gacgggcctc
     1081 gccgacgcgg cgctcaggct cggctcgcgg cctgcgggca tctgggcgcc cgagtgcggc
     1141 tacgcgcccg ggatggagca cgggtacgcg gccgccggtg tccggcggtt cctcgtcgac
     1201 ggtcccgcgc tgcgcggtga caccgcactc gcccgtcccg tcgggacctc ggacgtcctg
     1261 gccttcggcc gcgacctgca ggtcacctac cgcgtgtggt caccgcgctc gggctacccc
     1321 ggcggccgcg actaccgcga cttccacacg ttcgaccacc cgtccgggct caagccggcc
     1381 cgcgtcaccg ggcggtccgt ggctccggag gacaagcgcc cctacgagcc cgcccgcgcc
     1441 gcggcggcgg tggcccgcga cgccgccgac ttcgtggcgg tcgtccgcga ccggctcctc
     1501 cggctgcggg agcggctcgg ccggcccgcg ctgaccgttg ccgctttcga cacggagctg
     1561 ttcgggcact ggtggcacga gggccccgcc tggctggagg cggtgctgcg ggcgctgccg
     1621 gcggccggcg tccgggtgac gacgctgcgc ggggccgtcg acgcggggct ggtcggcgaa
     1681 ccggtcgagc tgccgccgtc gtcctggggg tcgggcaagg actggcgggt gtgggccggg
     1741 gagcaggtgg ccgacctggt ggcactcaac gagcaggtac agaagacgct gtcgggggca
     1801 gtcgcaccgg acgttgcgcg cgacccgctt cgcgacgcgc tcgcgcagga ggcgttgctc
     1861 gcactgtcca gtgactgggc cttcatggtc agcaaggact ccgccgccgg ctacgcgcgc
     1921 gaccgcgcgc acgggcacgc cggccgcgtc gaggagctgg cccgcctgct cgccgccgac
     1981 cggcgtcccg cagcggcccg ccgggtggcc tcctggcctc cggccccctt cggccacgtc
     2041 gacgcccgca ccctctgacg gttcggagaa cttctgcgcg cccggccgac gctgctcggc
     2101 cggggtaagc gcggccccgg cggggcacgt cgcctgctgt gcagccggcc gcccagcagg
     2161 ctcgcgccgc tgtcgggcca gtcgggcttc gcgtcgaacg ccatgatcac cgagtcgccg
     2221 atcctgactt ccgcgtggcc gatcgtgccg tcctggttca ggacgcgcag ggccgtgtct
     2281 gtgtcgaagg tctgctcgac gaagtcgagg aacgtcgccg ctcccttggc ggccacgtac
     2341 ggcgtcaccg tggacttgcc accggggatg gtcgttgttg cgtgtcgggc acgttcacat
     2401 cccactcctg ccggctgcgc ccggctaccg ccgggtaggt tgcgggggat gcgggtgctg
     2461 atggtgtcct gggagtaccc cccggtagtg atcgggggac tggggcggca cgtacacgcg
     2521 ctcgcggagg agctggcggc cgcagggcac gaggtcgtcg tgctctcccg gcagcccgcg
     2581 ggcaccgacg ccgcgaccca cccgacgctc gacgagctcg tcggcggcgt acgcgtgctg
     2641 cgcgttgcgg aggacccggc gcacctggag ttcgaacacg acatggtggc gtggacgctc
     2701 gcgatggggc acgcgctgct gcgcgccaca ctcacgcggc tcacggattg gcggccggac
     2761 gtcgtgcacg cccacgactg gctcgtggca catccggcga tcgcgctggc cgacgtgctc
     2821 ggggtgccgt tggtcgccac gatccatgcc accgaggccg gccggtacgc gggctggctg
     2881 tccgcaccgc tgagccggca ggtgcactcc gtcgaatggt ggctcgcgaa ccgcgccgac
     2941 gccgtgatca cgtgctcgtc cgcgatgcgg gacgaggttg ccgagctctt cgagctcgac
     3001 cccggccgga tcgcggtgct gcacaacggg atcgtgccgc gccgctggcg cacgagccgg
     3061 gcgcgcgtca cgagcgcccg gaaccggttc gcgccgggcg gcggcccgct cctgctgtac
     3121 ttcgggcggc tcgagtacga gaagggcgtg caggacctga ttgcggccct gcccagcatc
     3181 cgtagggcgc atcccggcac acgggcattg gtcgcgggca ccgggacagc tgcggagatg
     3241 ctggtggagt cggcgcgaag gcaccgcgtc cgtcgcagcg tcgactttct cggccatcta
     3301 cccgacgagg atctggtcgc actgctcgcg gcggccgatg cagtggtact gcccagccgc
     3361 tacgagccgt tcgggatcgt ggcgctggag gcggccgcgg caggggcgcc tctcgtggcg
     3421 tccacggcgg gcgggctcgg cgaggtcgtg gtcgatgggg agaccggaat gtcgttcgcc
     3481 ccgggggacg tggaagcgct ggcgcgcgcc gtcggctccg tgctcacgga cccgaaggcc
     3541 gcggagcggc gggccgacgc ggcgcgcacc cgactggcgg tcgacttcga ctgggcccgc
     3601 atcaccgcag gtacggcggc tgtgtacgcc gcaacccacg ccggcggggc catcgagtcg
     3661 ggccggccga agattccgac cggcaatgtg ttcggacgct gagcgccctg ggtggtgatc
     3721 aggctgcttc gtcgttcatg atctccagcg cggcgtcggg gtactccgac acgagctgct
     3781 ggatgaggtc gtcgcggtcc tccgcgacag ctgcgttcac ggcggccgtg tactcgtcgt
     3841 ctagggcgtt gagacggcga acggtgtcgt ccttcatggg gcacacacct tcctggctgg
     3901 cattatttct tccgacagct attgaacgcg actgagcgcg ccgtcgttcc ccctagttgt
     3961 gccaggtcac atcgattcag ctgcgttaac tctccttggg gatccgacta ccggacgttt
     4021 gctctacgcg ttcccgcacg gcggcctcgg tcttgcgcgc gatatcggcg agggcggccc
     4081 ggcgatcggc ggctgcatcg aagtcggcga gccggttccg caccacccgg gccggagcgc
     4141 cgacggccac cgagtactcc gggatgtcac cgcgtaccac cgcgtgcgcc ccgagcaccg
     4201 aaccccggcc gacccgggtg ccgcgcagca ccgacacctt cacgccgatc cagcagtcgg
     4261 gcccgatccg caccggcgtc ttgacgatgc cctggtcctt gatcgggacg cgtacgtcct
     4321 cgatgcggtg gtcgaagtcg gtgacgtaca cccagtccgc gacgatcgtg gacgcgccga
     4381 tctcgacgtc gaggtagcag ttgaccgtgt tgtccttgcc gaacacgacc ttgtcgccga
     4441 tccgcagcga cccttcgtgg caccgcagcg agttgccgtc gccgatgtgc acccaccgcc
     4501 cgatctcgag acgtccgtag cccggccggg cgtgcacctc cacccgcctg ccgaggaaca
     4561 ccatgccgcg caggatcacg tgggggttcg ccagccggaa ccgcagcagc cgccagtatc
     4621 ggacgaggta ccagggggtg taagcgcggt tgcggagcac ccagcgcagc gaggcgatgg
     4681 tgaggaaccg ggcctgccgg gggtcgcggc gaaaggcccg tccccaacgg tcacgcaggg
     4741 gcgctcccca catgctggtc atggatcgac gatagggcgg gagcgtttca ccggcgctcc
     4801 cacccgggta gcccgctccc gtgaccacgg agagacg
//
LOCUS       DASRSC010000013         6974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3934711,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000013 DASRSC010000000
VERSION     DASRSC010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6974
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3934711"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..940)
                     /locus_tag="VFE39_00435"
     CDS             complement(107..940)
                     /locus_tag="VFE39_00435"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET6257095.1"
                     /translation="MSRQDVLVTADWAEKNLGTEGIVFLEVDEDTTAYDGGHLPGAVK
                     VNWTTELQDPVRRDIVNKEQFEQLLSSKGIGNDDTVVLYGGNNNWFAAYAYWEFKLYG
                     HGSVKLLDGGRKKWELDGRTLTTDAAQRPATTYTAQEPDLSIRAFRDEVVEAIDNKNL
                     IDVRSPDEFSGKILAPAHLPQEQSQRPGHIPSAINIPWSKAANEDGTFKSDEDLKELY
                     GEAGYDEGKPTIAYCRIGERSSHTWVVLHELLGYSDVKNYDGSWTEYGSLIGVPIELG
                     D"
     gene            complement(937..1401)
                     /locus_tag="VFE39_00440"
     CDS             complement(937..1401)
                     /locus_tag="VFE39_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4395 domain-containing protein"
                     /protein_id="HET6257096.1"
                     /translation="MSTSAASARRDPPVDPRGVRFSASVTTVVLALVLITDSGLLLAA
                     QTVVFAFGSIAGLRFAPYSVLFRVLVAPRLAPPTQREDAAPVRFSQTVGLVFAVVGTA
                     GYLGGLTVLGVVATAFALAAAFLNAAFGFCLGCEMYALIARFRKTNQQGATT"
     gene            complement(1571..2014)
                     /locus_tag="VFE39_00445"
     CDS             complement(1571..2014)
                     /locus_tag="VFE39_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET6257097.1"
                     /translation="MISQPGLAVLAGTLVVATVVGWVLRARNGRLRTDGAATGGWVLA
                     GATPTGLDRVLLLQLSSPVCTPCRQTAALLTEFSARRPGVVHREVDAADRPEVARELF
                     VMRTPTVVAFDRDGAELLRVSGVPRLPELDAGIAPALIGTVGETS"
     gene            complement(2066..2848)
                     /locus_tag="VFE39_00450"
     CDS             complement(2066..2848)
                     /locus_tag="VFE39_00450"
                     /inference="COORDINATES: protein motif:HMM:NF022655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2993 domain-containing protein"
                     /protein_id="HET6257098.1"
                     /translation="MRRVVIGLLVLVGLLVAVDYGSAALAESAVSRQMREQIGLADDP
                     SVRINGFPFLTQAVSGHYRSVDIDAPRIHVGQLQELDVSAQLRDVTAPLSMLLGSGPK
                     TLRVQEAEGTVRVSASDVQRLLPGIEGLGIDSLDKKALQQAVKDGGQASLAQLDPAKV
                     ARFVGTGTVLGQQVQVSVIALLQLTDGQAEIVPQDIQLGDNAPALPAAVQRTLRQLFT
                     VRIDPGSLPLKVTPTALRANNGVLEISGVAQNLTLGATTPTS"
     gene            complement(2845..3588)
                     /locus_tag="VFE39_00455"
     CDS             complement(2845..3588)
                     /locus_tag="VFE39_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6257099.1"
                     /translation="MCHGFTHHTRHPVTRPVLDAMGASAPVVALDMRGHGRSGGRSTV
                     GDRELLDLDAAVKWARDAGYAHVATVGFSLGAAVALRQAATGAARPDAVVAVSGPARW
                     YSRETPAMRRVHWLLEQPHGRAIARLLGVRLDSSWEVVPPSPVEVVAAVAPVPLLLVH
                     FAGDHYFSAAHAQALVTASGGHAQLWTEPGAGHGESGTDPGLAARIARWAVAAAATGA
                     SPGDATSGDGSTVRLLTPADRRAPGSEVG"
     gene            3855..4634
                     /locus_tag="VFE39_00460"
     CDS             3855..4634
                     /locus_tag="VFE39_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6257100.1"
                     /translation="MELLLLTADPDPGTVLPALTLLPHGVRTAAPEVAALLDAGPHDA
                     VLVDARTDLVAARSLCRLLGSTGMDVPVIAVLTEGGLVAVSGEWVVDEILLPGAGPAE
                     VDARLRLLKSRPGADTGRDGGALVLGELVIDEATYAARLRGRLLELTYKEFELLKYLA
                     QHAGRVFTRAQLLQEVWGYDFFGGTRTVDVHVRRLRAKLGTEHEQMIGTVRNVGYKFV
                     RPSRGGLGAPAVGIVEDDGGNDDGEEGDRVPGRVSGVAGMA"
     gene            4720..5601
                     /gene="mshD"
                     /locus_tag="VFE39_00465"
     CDS             4720..5601
                     /gene="mshD"
                     /locus_tag="VFE39_00465"
                     /EC_number="2.3.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035447 - mycothiol synthase activity
                     [Evidence IEA];
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol synthase"
                     /protein_id="HET6257101.1"
                     /translation="MDETAIAEVTALASAATDADGTAPLGEHVLLHLSDDTVVHLLAH
                     DPDGALVGFAHLDLTGPPPDGAAELVVHPAHRRGGVGTRIVEALLDRTAGGHLRVWAH
                     GQHPGAVALAARLAFTPVRVLWQMRRSLLTPLAEPHLPDGVRLRSFVVGGDEPEFLRV
                     NNAAFDWHPEQGGWDVDQVKLRESESWFDPAGFLLAVDGTDRLLGYHWTKVHDESDAG
                     EHGPEPIGEVYVLGVDPAARGRRLGAALTLAGLQYLRDRGLSQAMLYVEADNSAAVRV
                     YENLGFALWGTDVSYAR"
     gene            5834..6952
                     /gene="pstS"
                     /locus_tag="VFE39_00470"
     CDS             5834..6952
                     /gene="pstS"
                     /locus_tag="VFE39_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET6257102.1"
                     /translation="MKTVRHGARARIAAVGIATSGVLLIAGCGAANESAPSGGAGGSA
                     APSVSGTIAGAGSSAQQAAQEAWIATFTDQNANATITYDPSGSGAGRTQFLGGGVAFA
                     GSDVYLTSAELTQAKTRCGGDVVEVPDYVSPIAVIYNVPGVSNLQLAPATIAQIFDRK
                     ITKWNDPAIAADNPGATLPDLAITPVNRSDKSGTTQNFADYLKVAAGPAWPYAVSDTW
                     PVQGGEAAQGTSGVVGAVKAGNGSIGYADESQARDMGQAKVKVGSEYVGATPEAAAAV
                     LDASPRVDGQGQYAFAYKLARDTTASGTYPVVLVSYLLACSKYADAPTANLVKAYLNY
                     VVSADGQSLAASKAGAAPLSDTLRGQITPAINAIAAGS"
BASE COUNT         1042 a         2432 c         2460 g         1040 t
ORIGIN      
        1 ggcgacgggg ttgccgccca gccggacgcg gccgccgatc acggtctcct tgctcacgtc
       61 gacgccctca agcgagacgc cgccggcgag cggggcacag cccacgtcag tcgcccagct
      121 cgatcggcac accgatcagc gagccgtact cggtccagct gccgtcgtag ttcttgacgt
      181 cgctgtagcc gagcagctcg tgcagcacga cccatgtgtg ggagctgcgc tcgccgatgc
      241 ggcagtaggc gatggtcggc ttgccctcgt cgtagcctgc ctcgccgtag agctccttga
      301 ggtcctcgtc ggacttgaag gtgccgtcct cgttggccgc cttgctccac gggatgttga
      361 tcgcggaggg gatgtgcccg gggcgctggg actgctcctg gggcaggtgt gccggagcga
      421 ggatcttgcc ggagaactcg tcgggcgagc gcacgtcgat caggttcttg ttgtcgatcg
      481 cctcgacgac ctcgtcccgg aaggcccgga tcgacaggtc gggctcctgc gccgtgtagg
      541 tggtggccgg ccgctgggcg gcgtcggtgg tgagcgtgcg gccgtcgagc tcccacttct
      601 tgcgcccgcc gtcgagcagc ttgacggatc cgtggccgta gagcttgaac tcccagtacg
      661 cgtacgcggc aaaccagttg ttgttaccgc cgtagagcac gacggtgtcg tcgttgccga
      721 tgcccttcga cgacagcagt tgctcgaact gctccttgtt gacgatgtcg cggcgcaccg
      781 ggtcctggag ctcggtcgtc cagttgacct tgactgctcc ggggaggtgc ccaccgtcgt
      841 aggcggtggt gtcctcgtcg acctcgagga agacgatccc ctcggttccg aggttcttct
      901 cggcccaatc tgctgtgacc aggacgtcct gacggctcat gtggtggctc cctgctggtt
      961 ggtcttcctg aaacgggcga tcagggcgta catctcgcag cccaggcaga agccgaaggc
     1021 ggcgttgagg aatgcggcgg ccagtgcgaa tgcggtggcg accacgccga ggacggtcag
     1081 gccccccaga tacccggccg tcccgacgac ggcgaacacg aggccgaccg tctgggagaa
     1141 ccgcaccggg gcggcatcct cccgttgtgt gggtggtgcg agccgcggcg cgacgagcac
     1201 ccggaacaga accgagtacg gcgcgaagcg gagcccggcg atcgagccga acgcgaacac
     1261 gacggtctga gcggcgagta acaagccgct gtcggtgatg agcacgagtg cgagcacgac
     1321 ggtggtgacc gatgccgaga agcggacacc gcgcggatcg accggcggat ctcggcgcgc
     1381 ggacgcggcg gaagtagaca tggagatgct cctgagaacg aggtgacggg acggggatct
     1441 caggaggtcg gacagaggca gctgccgacg cggcacaggt caactgcgcg tcgtcgggtc
     1501 agcggccagc acacggtggc gagggtaatg gaggatccgg cgtttcggga ggtccgtcgg
     1561 tccggaccgg tcatgatgtt tccccgaccg tcccgatcag ggcaggcgca atcccggcgt
     1621 ccagctcggg gagccgtggc accccggaca cgcgcagcag ctcggcgccg tcgcggtcga
     1681 acgccaccac cgtcggcgtc cgcatcacga agagctcgcg cgcgacctcc ggccggtcgg
     1741 cggcgtcgac ctcgcggtgc acgacgccgg ggcggcgtgc ggagaactcg gtcagcagcg
     1801 cggccgtctg gcggcacggc gtgcagacgg gggaggagag ctgcagcaag agcacccggt
     1861 ccaacccggt tggggtcgct ccggccagta cccatccgcc ggtcgcggcc ccgtcggtgc
     1921 gcaaccgccc gttgcgggcg cgcagcaccc agccgaccac cgtggcgacc accagcgtgc
     1981 ccgccagcac cgctagtccc ggctgactga tcatggctgc tccaacgcgc ttgcggagtc
     2041 cgttactccc ggccgtcacc gggattcagg aggtgggtgt cgtggcacca agggtgaggt
     2101 tctgggcgac accgctgatc tccagtacgc cgttgttcgc ccggagcgcc gtgggggtga
     2161 ccttcagcgg cagcgaaccg ggatcgatgc gcacggtgaa cagctgccgg agcgtccgct
     2221 gcaccgccgc cgggagcgcc ggcgcattgt ccccgagctg gatgtcctgt gggacgatct
     2281 cggcttggcc gtccgtcagc tggagtaggg cgatcactga tacctggacc tgctggccca
     2341 agaccgtgcc cgtacccacg aaccgtgcga ccttggcggg atcgagctgc gccaacgacg
     2401 cctggccacc gtccttgacg gcctgctgca gggccttctt gtcgagcgag tcgatcccca
     2461 ggccctcgat gccgggaagg aggcgctgca cgtcgctggc ggacacccgc acagtgccct
     2521 cggcctcctg cacgcgaagc gtcttcgggc cagagccgag cagcatcgac agcggggccg
     2581 tcacgtcccg cagctgcgcc gagacgtcga gctcctgcag ttgcccgacg tggatccgcg
     2641 gcgcatcgat gtcgacgctc cggtagtggc cggacacggc ctgggtgagg aacgggaaac
     2701 cgttgatccg cacggagggg tcgtcggcga gcccgatctg ctcccgcatc tggcgtgaca
     2761 ccgccgactc ggcgagggcg gcggatccgt agtccacggc cacgagcagc cccacgagca
     2821 cgagcagccc gatgaccacg cgcctcatcc gacctccgat cccggtgccc ggcgatctgc
     2881 cggggtgagg agtctgaccg tagagccgtc acctgaggtc gcgtcgccgg ggctcgcacc
     2941 cgtggccgcg gcggcgaccg cccacctcgc gattcgcgcg gcaaggccgg gatcggtgcc
     3001 gctctcgccg tggccggcgc ccggctcggt ccacagctgt gcgtgcccgc ccgacgccgt
     3061 cacgagcgcc tgcgcgtgcg ccgcggagaa gtagtggtcg ccggcgaagt ggacgagtag
     3121 caggggcacg ggtgccaccg ccgcgacaac ctccaccggg gacggcggga ccacctccca
     3181 cgacgagtcc aggcgcacgc cgagcaggcg ggcgatggcg cggccgtgcg gctgctccag
     3241 caaccagtgc acgcgccgca tcgccggggt ctcgcgggag taccagcggg caggtccgct
     3301 gacggcgacg acggcgtcgg ggcgggcggc tcccgtggcg gcctgccgca atgccaccgc
     3361 ggcccccagc gagaacccga ccgtcgccac gtgcgcgtat ccggcgtcgc gggcccactt
     3421 cacggccgca tcgaggtcga gtagctcacg gtcccccacc gtgctgcgcc cgccggagcg
     3481 cccgtgtcca cgcatgtcga gggccacgac gggggcgctc gcgcccatcg cgtcgagcac
     3541 cggccgggtg acgggatggc gggtgtgatg ggtgaagcca tgacacacag cgatgatggt
     3601 gtccccgccc ggccccgaat ggtgtgatcc ggacaaaggc acaccgtccg aactggtcac
     3661 gttcaccgaa cagggcgtga cctccaccgc ggcgctcgtg ccttgggccc tcacccggtt
     3721 atcctgcatt gatccgcgtg gtggtgttcc aaccgggtca cgtgggtgtg aacggcgtca
     3781 gcgtcgcccg ccgtgatcgg acgagggacg ccgaaccggg cgccccggct cccggtgtgg
     3841 aaaggaggca ccacgtggaa ctccttctgc tgaccgccga tcccgatccg gggaccgtgc
     3901 tgccggctct gacgcttctc ccgcacgggg tccgcacggc tgctcccgag gttgccgcac
     3961 tgctcgacgc cggtccgcac gacgccgtgc tggtcgacgc gcgcaccgat cttgtggcag
     4021 cccgcagcct gtgccgcctg ctcggcagca cggggatgga cgtgccggtc atcgcggtgc
     4081 ttaccgaggg cggactggtg gcggtatccg gcgagtgggt cgtggacgag atcctcctgc
     4141 ccggcgccgg tcccgccgag gtcgatgcgc ggctgcgcct gctcaagtcc cgtcccggtg
     4201 ccgacacggg ccgtgacggc ggagccctgg tgctcggcga gctcgtgatc gacgaggcca
     4261 cctacgccgc gcgcctccgc ggcaggctcc tcgagctcac gtacaaggag ttcgagctgc
     4321 tcaagtacct cgcgcagcac gccggccggg tgttcacccg cgcccagctg ctgcaggagg
     4381 tctggggcta cgacttcttc ggcggcacgc gcactgtgga cgtccacgtg cgacggttgc
     4441 gcgccaagct cggtacggag cacgagcaga tgatcggcac cgtgcgcaac gtcggctata
     4501 agttcgtccg gcccagccgt ggcgggctgg gagcgcctgc cgtcggcatc gtcgaggacg
     4561 acggcggcaa cgacgacggc gaggagggcg accgggtccc gggccgagtc tcgggcgtcg
     4621 ccgggatggc gtgaccacca cctccgcgcc gggtacgcga ccgtccggtg ctccaggggt
     4681 accgtcgatc gtgtgaccga gttgtcgtgg cgcgccgggt tggacgagac cgccatcgcc
     4741 gaagtcacgg ctctcgcatc agccgccact gacgccgacg gcacggcgcc gctgggtgag
     4801 cacgtgctgc tgcacctgtc cgacgacacc gtcgtccacc tgctcgccca tgaccccgac
     4861 ggcgccctcg tcgggttcgc gcacctcgat ctgaccggcc caccgcccga tggcgcggca
     4921 gagttggtcg tccatcccgc acatcgccgc ggcggtgtgg gcactcgcat cgtcgaggcg
     4981 ctgctggatc gcacggccgg cgggcacctg cgcgtgtggg cccacggaca gcaccccgga
     5041 gcggtcgccc tcgccgcgcg cctcgctttc accccggtgc gggtgctctg gcaaatgcgc
     5101 cggagcctgc tcaccccgct cgccgagccc cacctgccgg acggcgtgcg gttgcgttcg
     5161 ttcgtcgtgg gcggggacga gcccgagttc ctgcgcgtca acaatgcggc gttcgactgg
     5221 catcccgagc agggcggctg ggacgtcgac caggtcaagc tgcgggaatc cgagtcgtgg
     5281 ttcgatcccg caggcttttt gctggccgtg gacggcacgg accggctgct cggttaccac
     5341 tggacgaagg tgcacgacga gtcggacgcc ggggagcacg gaccggaacc gatcggcgag
     5401 gtctacgtgc tcggcgtcga cccggcggcg cgcggcagac gcctcggtgc ggccctcacc
     5461 ctcgccgggc tgcagtacct gcgagaccgt ggcctcagcc aggcgatgct ctacgtcgag
     5521 gccgacaaca gcgctgcggt gcgggtctac gagaacctcg gcttcgccct ctggggcacg
     5581 gatgtttctt acgcgcggtg acaaccgttc atcccccggc aaccatggcg gcaccagagg
     5641 cgatcacatc gccgcaggtc acgtctaggt cacggcgccc ttcttgggga tgtcatggcc
     5701 gacatcaggt gagctgttca tttcgcgtta acgggatcgg gggggtgtgg ccaccccgct
     5761 tacctaccgt cgccgtcgtg cggccacccc gagccgcgta cccggttacg ggacacatga
     5821 cggaggaaca cacgtgaaga ccgtccggca cggcgctcgt gcccgaatcg ccgcggtggg
     5881 catcgccacc agcggcgtac tgctgatcgc cggctgtgga gcagccaacg agtcggctcc
     5941 gtcgggtggg gccggtggat ccgcggcacc gagcgtctcc ggaacgatcg ccggcgccgg
     6001 ttccagtgct cagcaagcgg cccaggaggc gtggatcgcg accttcacgg accagaacgc
     6061 caacgcgacc atcacgtacg accccagcgg atccggggcc gggcgcactc agttcctcgg
     6121 cggcggcgtc gcgttcgccg gtagcgacgt ctacctcacg tccgcagagc tcacccaggc
     6181 gaaaacgcgc tgcggcggcg acgtcgtcga ggttccggac tacgtctcgc cgatcgcggt
     6241 gatctacaac gttccgggcg tgagcaacct gcagttggcc ccggccacga tcgcgcagat
     6301 cttcgaccgg aagatcacca agtggaacga cccggccatc gccgccgaca atcctggcgc
     6361 cacgctgccg gacctcgcga tcaccccggt gaaccgctct gacaagtccg gcaccacgca
     6421 gaacttcgcc gactacctga aggtggctgc cggcccggcg tggccgtacg ccgtcagcga
     6481 cacctggccc gtacagggcg gggaagccgc ccagggcacc tcgggtgtcg tcggcgcggt
     6541 gaaggccggc aacggctcga tcggctacgc cgacgagagc caggcccgcg acatgggcca
     6601 ggccaaggtc aaggtgggct cggagtacgt gggagccacc cctgaggccg cagcggccgt
     6661 gctcgacgcc tctccccggg tcgacgggca gggccagtac gccttcgcct acaagctcgc
     6721 ccgggacacc accgcgtcgg gtacctaccc ggtggtgctc gtgtcctacc tcctggcctg
     6781 ctcgaagtac gcggatgctc ccaccgcgaa cctggtgaag gcctacctga actacgtcgt
     6841 cagtgctgac ggccagtccc tcgccgcgag caaggccgga gcggctccgc tcagcgacac
     6901 gctccgcggc cagatcaccc cggccatcaa tgccatcgcc gcgggttctt gagatttcca
     6961 tcgtctagct gtcg
//
LOCUS       DASRSC010000014         6662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2951887,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000014 DASRSC010000000
VERSION     DASRSC010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6662
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2951887"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFE39_00475"
     CDS             complement(<1..488)
                     /locus_tag="VFE39_00475"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257103.1"
                     /translation="MTGATIGAVTTGAATTGAATTGAATTGAATVHLVPHTHWDREWY
                     EPFQRFRLRLVDLLDEVLEWAEADRRFRFTLDGQMAAVDDYLEVRGENRGRIAALVRR
                     GQLAVGPWQILLDEFLVSGENIIRNLELGRARADELGGAMPVGYLPDEFGHCAQMPQI
                     LAA"
     gene            complement(586..1314)
                     /locus_tag="VFE39_00480"
     CDS             complement(586..1314)
                     /locus_tag="VFE39_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HET6257104.1"
                     /translation="MTLTPGGTTHRRTLRWQTARVLTVRDETPRARTFRLALPEPRLH
                     LAGQHYVLRLTAPDGYTASRSYSVASAPDGTGEIELTVERLDGGEVSEFLHDVVQPGD
                     ELEVRGPIGGFFVWPGDTPALLVGGGSGVVPLMAMLRQARASGHPDLLRLVVSARSPG
                     ELYYADELPGPETVVVYTRQAPPGAGRTAGRIAVADVAPLVRGGEDVYVCGSPGFADA
                     ATAVLLEAGVPVHRIRVERFGPSG"
     gene            complement(1329..1937)
                     /locus_tag="VFE39_00485"
     CDS             complement(1329..1937)
                     /locus_tag="VFE39_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HET6257105.1"
                     /translation="MAGITRGFLGRRGERDPRLPPGQYDARDQWPVLHAEATPRISTD
                     TWTFTVEGLVEHEVSWTWDEIHALPRARYDGAIHCVTTWSKFDMTFDGVSVDTLLEAA
                     RPLPSTTHVMAVSHTGYTTNLPLADVTDGKAWVVWDADGAPLTVEHGGPARLLVPHLY
                     FWKSAKWVSGLRLVDHDEPGFWERNGYHDRGDPWAEQRYQGD"
     gene            complement(1966..2562)
                     /locus_tag="VFE39_00490"
     CDS             complement(1966..2562)
                     /locus_tag="VFE39_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET6257106.1"
                     /translation="MSAVRLSPTSYVILGMIALRGPSTPYDLKRAVGHSVGYFWNFPH
                     AQLYSEPDRLADLGLLALSVEDSGRRRKTYSLTDLGRAALRAWLAAPTDEHFQMRDIA
                     ELKLFFNEVGDPDNVPSLAAEQIKQHEERIAVYEGMVERFGDDPASRPRMITLELGLE
                     MEHAALRFWTALAEGDLDRLRRARMGTTAGTTSATTAE"
     gene            2665..4173
                     /locus_tag="VFE39_00495"
     CDS             2665..4173
                     /locus_tag="VFE39_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6257107.1"
                     /translation="MDLATALGWAAQRHPDRRAVGGAGRHLTYRQWDAHTNQLARALA
                     GVGVRPGDRVALSLAGGEPLASLHLAAQKLGAVSVPLSTRFGPDELGYCLGDAAAVLL
                     VTDATTAERAAGAAGEIPVRDAGELDARGADEPDGALSEVPAEDTVSVMLYTSGTTGR
                     PKGVPRTHRAEHTAAVAHAIQTQQRQGEVVLGVMPMFHTMGLRTLLASVVVGGTWVGQ
                     PDFDPEGSLELMRAENVTSLYLVPTIYWSLLHSGRLGEARTLDRLAYAGAAMTPTLAE
                     QLVDTVAPTAFVNHFGSTEIYTFTIGPDVAAKPGSAGRAGIFSQVRLVSPEPGAPPDA
                     LVGPGEQGQVIVSMASPEAFGGYWQRPDADEKAIRDGWYYTGDLATTDDDGDLWVAGR
                     VDDMINSGGENIYPDEIEAALVRCAAVADVCVVGLPDERWGHAVTAFVVPARGTDPAA
                     AVSATAAHARSVLPSLKRPKRVIAVEAIPRSGVGKTLRRTLVAGEFEALADV"
     gene            4265..>6662
                     /locus_tag="VFE39_00500"
     CDS             4265..>6662
                     /locus_tag="VFE39_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET6257108.1"
                     /translation="MPSRIEDGPLVAGRGRFLDDMDPLPGTLVAAVVRSTQPHARLRS
                     VDLSRARSYPGVAAVVGPDEVRAALRPFPLSTGTAMPYYPTGVDKVRFVGEPIAVVVA
                     TDRFTAEDAAELVRVDYEPLDVVVGARAALAPGAPLLHEAAGSNVATDRTFRFGPVQE
                     AFASAARGGGHVVEGEYDFPRYSSVPMECYSVIANWQHTDSGPGVEAWANFHGPFTMV
                     PVMAGALGLPTSAVRLHVPADIGGSFGIKAGIYPYVVLMALASKHAGTPVRWTEDRVE
                     HLLASSSGSDRAMRFAAAVTQSGEVTALRVDLVDNVGAYLRPPEPSTLYRCFGNITGP
                     YRIGAVEIRARAVVTNSMPTGLNRGFGGQQLYFGLERLMDAVAGATGLDVLEVRRRNL
                     VDTFPYESPTGGCYDSGDYARALELAVRAADLDELRARQTAARAEGLHYGIGVGLVVD
                     PSGTNIGYVGLATPAEERRPGRDKSGSTEHVRISVDMQGIVTVMLGSVPQGQGHATVA
                     RQVAADRLGLPVEQVRVVVDMDTATTPWTVTSGSYSSRFAPLVTSAVVQAADRIAATL
                     RAAASVMLDLPPDELSLVDGTLRHPSGPSVAFRHAAGVVHWDPASLPAGTSARLYEEA
                     AFTPHETSAATADDRINSSLCYGFVAEIVALRIDPDTLEIAIDRVSSVHDAGTVLNQQ
                     LLDGQVHGALVHALGGAQYEEFTYAPSGQPTSATFLDYLCPTSAETAYDLRTDHLVSP
                     SPLTPLGAKGCGEGSSMSFPVAFANAVADALAPSGVEITRLPLHGEVLHQLMEKNEKE
                     T"
BASE COUNT          898 a         2343 c         2474 g          947 t
ORIGIN      
        1 gcggcgagga tctgcggcat ctgcgcgcag tggccgaact cgtcgggcag gtaccccacc
       61 ggcatcgccc cgccgagctc atcggcccgc gcgcggccca gctccaggtt ccggatgatg
      121 ttctcgccgg agaccaggaa ctcgtcgagc aggatctgcc acgggccgac ggcgagctgg
      181 ccacgccgta caagcgccgc gatccggccg cggttctcgc cgcgcacctc gagatagtcg
      241 tcgaccgcgg ccatctgacc gtccagggtg aagcggaagc gccggtccgc ctcggcccac
      301 tcgagcacct cgtcgagcag gtcgaccagc cggagccgga agcgctggaa cggctcgtac
      361 cattcccggt cccaatgcgt gtggggaacc aggtggacgg tcgccgctcc agttgtcgcc
      421 gctccagttg tcgccgcccc ggttgtcgcc gctcctgttg tcacggcccc gatcgtcgct
      481 ccggtcatgc gctggctgcc tcgcttcgat cagggtgccg gacctgttgg tcctgacaac
      541 ctaacgatca ctagccagta tgtcgacgtc ggtcggtttg tcccgctaac cggaggggcc
      601 gaaccgctcc acccggatcc ggtggaccgg tacgcctgcc tccagcagca cggccgtggc
      661 cgcgtcggcg aagccggggg agccgcacac gtacacgtcc tcgccaccac ggaccagtgg
      721 cgcgacgtcg gcgacggcga tgcggcccgc ggtgcggcct gcaccgggcg gggcctgccg
      781 ggtgtacacg accaccgtct cgggaccggg gagctcgtcg gcgtagtaca gctcgccggg
      841 ggagcgggcc gacaccacga ggcgcaggag atccgggtgg cccgaggccc gcgcctggcg
      901 cagcatcgcc atcagcggca ccacgccgga gccgccgccg acgagcaggg caggcgtgtc
      961 gccgggccag acgaagaaac cgccgatcgg tccgcggacc tccaactcgt cgccgggttg
     1021 gaccacgtcg tgcaggaact ccgagacctc gccgccgtcc agtcgctcga ctgtcagctc
     1081 gatctcgccg gtcccgtcgg gcgccgaggc gaccgagtac gagcgggagg ccgtgtagcc
     1141 gtccggcgcg gtgagccgca ggacgtagtg ctgcccggcg aggtgcaggc gtggttccgg
     1201 cagcgcgagc cggaaggtgc gcgcccgggg cgtctcgtcg cggacggtga gcacccgggc
     1261 ggtctgccag cgcagggtcc tgcggtgggt ggtgccaccg ggggtgaggg tcatcgacgg
     1321 cccaccggtc agtcgccctg gtagcgctgc tcggcccagg ggtcgccgcg gtcgtggtag
     1381 ccgttgcgct cccagaagcc cggctcgtcg tggtccacca ggcgcaggcc cgacacccac
     1441 ttcgcgctct tccagaagta gaggtgcggc accagtagcc gggcggggcc gccgtgttcg
     1501 acggtgagcg gggcgccgtc ggcgtcccag accacccacg ccttgccgtc ggtgacgtcg
     1561 gcgagcggca ggttcgtggt gtagcccgtg tgcgacacag ccatcacgtg cgtggtgctg
     1621 ggcagcggac gcgccgcctc gagcagcgtg tcgaccgaga cgccgtcgaa ggtcatgtcg
     1681 aacttcgacc aggtggtgac gcagtggatg gcaccgtcgt agcgggcgcg gggcagcgcg
     1741 tggatctcgt cccacgtcca gctcacctcg tgctcgacca gtccctccac ggtgaacgtc
     1801 caggtgtcgg tggagatccg cggcgtggcc tcggcgtgca gcacgggcca ctggtcgcgt
     1861 gcgtcatact ggccaggcgg aaggcggggg tcgcgttcgc cgcgccgtcc caggaaaccg
     1921 cgtgtgatgc ccgccatggt ctgctgaagc ctccgggagc ccgtttcatt ccgccgtggt
     1981 ggcgctcgtc gtgccagccg tcgtccccat gcgtgcccgg cgcaagcggt cgaggtcgcc
     2041 ctcggcgagt gccgtccaga accgtagcgc ggcgtgctcc atctcgagcc ccagctccag
     2101 ggtgatcatc cggggccggc tcgccggatc gtcgccgaag cgctcgacca tcccctcgta
     2161 caccgcgatg cgctcctcgt gctgcttgat ctgctcggcg gcgaggctcg ggacgttgtc
     2221 cgggtcgccc acctcgttga agaacagctt gagctccgcg atgtcgcgca tctggaagtg
     2281 ctcgtcggtc ggagcggcga gccatgcccg cagggcggca cggccgaggt cggtgaggga
     2341 gtaggtcttg cgccgccgcc cgctgtcctc gacggacagg gcgagcagcc cgaggtcggc
     2401 gaggcggtcg ggttccgagt acagctgtgc gtgcgggaag ttccagaagt agccgaccga
     2461 atgccccacg gcgcgcttga ggtcgtaggg agtggacggg ccgcggaggg cgatcatccc
     2521 gaggatgacg tacgacgtcg gggacagccg gaccgcggac actcgcgaga tcctagccga
     2581 acggtccggg taggaccgtc cggagttgcc ggttgaacct ctcgacagta ccgtccgaga
     2641 cggatggttg accgagggag acccatggat ctggccacgg cactgggctg ggcggcgcag
     2701 cgccatcccg accgccgtgc ggtcggcggg gcggggcggc acctgacata tcggcagtgg
     2761 gacgcccaca cgaaccagct cgccagggcg ctggccggtg tgggcgtgcg gccgggcgac
     2821 cgcgtggccc tctcgctcgc cggtggggaa ccgctggcca gcctccacct ggccgctcag
     2881 aagctcggtg cggtgtcggt gcccttgtcc acccggttcg gccctgacga gctgggctac
     2941 tgcctcggcg acgcggccgc ggtgctgctg gtgaccgacg ccaccacggc ggagcgggcg
     3001 gccggcgccg cgggtgagat ccccgtccgc gacgcggggg agctcgacgc ccgcggcgcc
     3061 gacgaaccgg acggcgcgct gtcggaggtt cccgccgagg acacggtcag cgtgatgctc
     3121 tacacctccg gcaccaccgg gcggccgaag ggcgtccctc gcactcaccg cgccgagcac
     3181 accgctgcgg tggcgcacgc gatccagacc cagcaacgac agggcgaggt ggtgctcggc
     3241 gtgatgccga tgttccacac gatgggtctg cgcaccctgc tggccagcgt cgtcgtcggc
     3301 ggcacctggg tcggtcagcc cgacttcgac cccgaaggct cgctggagct gatgcgcgcc
     3361 gagaacgtga cctctctgta cctggtgccg acgatctact ggtcgctgct gcacagcggg
     3421 cgcctcggcg aggcgcggac cctggaccgg ctcgcctacg cgggcgcggc gatgaccccg
     3481 acccttgccg agcagctcgt cgacacagtg gcgcccacgg cgttcgtcaa ccacttcggc
     3541 agcactgaga tctacacgtt cacgatcggg ccggacgtgg cggccaagcc gggttcggcc
     3601 gggcgggccg ggatcttctc gcaggtccgg ctggtctccc ccgaacccgg tgcgcccccg
     3661 gacgcgctgg tcgggccggg ggagcagggg caggtgatcg tgtcgatggc gagcccggag
     3721 gcgttcggcg ggtactggca gcggccggac gcggacgaga aggcgatccg ggacggctgg
     3781 tactacaccg gtgacctggc gacgaccgac gacgacggcg acctgtgggt ggccggccgg
     3841 gtcgacgaca tgatcaactc gggcggcgag aacatctacc ccgacgagat cgaggcggcg
     3901 ctcgtgcgct gtgccgccgt tgccgacgtg tgcgtcgtgg ggctgcccga cgagcggtgg
     3961 gggcacgctg tcaccgcgtt cgtcgtgccg gcgcggggca ccgacccggc ggcggccgtg
     4021 tcggcgaccg cagcgcacgc ccgctcggtg ctgccgtcgc tgaagcgccc gaagcgggtg
     4081 atcgcggtcg aggcgatccc ccggtccggg gtgggcaaga cactgcggcg cactctcgtg
     4141 gccggtgagt tcgaggcgct ggccgatgtc tgagtcggcc caggggaggg cggcccaggg
     4201 gagggcggcc caggggaggg cggcgcaggg gagggcggcg caggggggag cggcacagcg
     4261 ctgggtgccg tcccggatcg aggacggccc gctggttgca ggtcgcggcc ggttcctcga
     4321 cgacatggac ccgctgcccg gcacgctcgt cgccgcggtg gtccgctcca cgcagccgca
     4381 cgcccggttg cgctcggtcg acctgtcccg ggcgcggtcg tatccgggtg tcgccgcggt
     4441 ggtcgggccg gacgaggtgc gcgccgcgct gcggcccttc ccgctgtcca ccggcaccgc
     4501 catgccctac tacccgaccg gagtcgacaa ggtgcgcttc gtcggggagc cgatcgcggt
     4561 cgtcgtcgcc acggaccggt tcaccgccga ggacgccgcc gagctcgtgc gggtggacta
     4621 cgagccgctc gacgtcgtgg tcggcgctcg cgcggccctg gcgcccggtg cgccactgct
     4681 gcacgaggcg gcgggctcga acgtcgcgac cgaccggacc ttccggttcg gcccggtgca
     4741 ggaggcgttc gcgagcgcgg cgcgaggcgg cgggcacgtc gtcgaaggcg agtacgactt
     4801 cccgcgctac tcgtccgtgc ccatggagtg ctacagcgtc atcgcgaact ggcagcacac
     4861 cgacagcggg ccgggtgtcg aggcgtgggc caacttccac ggcccgttca cgatggtccc
     4921 ggtgatggcc ggggcgctgg gccttccgac gtccgcggtt cggctgcacg tgccggccga
     4981 catcggcggg agcttcggca tcaaggccgg gatctacccg tacgtggtgc tgatggcgct
     5041 ggcctcgaag cacgcgggca cgcccgtgcg ctggaccgag gaccgtgtcg agcacctgct
     5101 ggccagctcg tcgggatcgg accgggcgat gcgcttcgcg gcggcggtca cgcagtccgg
     5161 cgaggtgacg gcgctgcggg tggatctcgt cgacaacgtc ggcgcctacc tgcgtccgcc
     5221 ggagccgtcc acgctgtacc gctgcttcgg caacatcacc gggccgtacc ggatcggggc
     5281 ggtcgagatc agggcccgcg ccgtggtcac caactccatg ccgacggggc tcaaccgcgg
     5341 gttcggcgga cagcagttgt acttcgggct ggaacgcctc atggacgcgg tggccggggc
     5401 gaccgggctc gacgtgctgg aggtccggcg gcgcaacctc gtcgacacct tcccctacga
     5461 gtcgcccacc ggcggctgct acgactcggg cgactacgcg cgtgcgctcg aacttgcggt
     5521 gcgggccgcc gatctcgacg agctgcgggc tcggcagacg gcggcccggg ccgaggggct
     5581 gcactacggc atcggggtcg ggctggtcgt cgacccctct ggcacgaaca tcggctatgt
     5641 cgggctcgcg acgcccgccg aggagcgccg accgggacgg gacaagtccg gctccaccga
     5701 gcacgtgcgg atctcggtcg acatgcaggg gatcgtcacg gtgatgctcg gctcggtgcc
     5761 ccagggccag ggccacgcca ctgtggcccg ccaggtggcg gccgaccgcc tcgggctgcc
     5821 cgtcgagcag gtgcgggtcg tcgtcgacat ggacaccgcc accacgccgt ggacggtgac
     5881 gtcggggagc tattcgtcgc gattcgcgcc actggtcacc agcgccgtcg tgcaggcggc
     5941 cgaccggatc gcagcgacgc tgcgggcggc ggcgtcggtg atgctcgacc tgccccccga
     6001 cgagctgtcg ctggtggacg gcaccctccg gcacccctcc ggcccgagcg tggcgttccg
     6061 gcacgcggcc ggggtggtgc actgggaccc ggcgtcgctg ccggcgggca cctcggcccg
     6121 gctctacgag gaggcggcgt tcaccccgca cgagaccagc gccgccaccg ccgacgaccg
     6181 catcaactcc tcgctctgct acggcttcgt cgccgagatc gtcgccttgc ggatcgaccc
     6241 cgacaccctc gagatcgcga tcgaccgcgt ctcgtcggta cacgacgccg ggactgtgct
     6301 caaccagcag ctcctcgacg gtcaggtgca cggcgcgctg gtgcacgcgc tcggcggggc
     6361 gcagtacgag gagttcacct acgcgccctc cgggcagccc acctcggcga cgttcctcga
     6421 ctacctctgt cccaccagcg ccgagaccgc ctacgacctg cgtaccgacc acctcgtcag
     6481 tccgtcgccg ctcaccccgc tcggggcgaa gggctgcggc gagggctcgt cgatgagctt
     6541 cccggtcgcg ttcgccaacg ccgtggcgga tgcgctcgcg ccgtccggcg tggagatcac
     6601 ccggctgccg ctgcacggag aggtgctgca ccagctcatg gagaagaacg agaaggagac
     6661 cc
//
LOCUS       DASRSC010000015        14679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1642206,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000015 DASRSC010000000
VERSION     DASRSC010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 14679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14679
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642206"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VFE39_00505"
     CDS             <1..258
                     /locus_tag="VFE39_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HET6257109.1"
                     /translation="GYQLATGNSVMPVGGYNGTDPSPTLQQFQQLVAERQIHWFIVGG
                     MMGGPSASGSDQSQQISAWVAATFTPVTVDGVTLYDLSAAS"
     gene            348..1613
                     /locus_tag="VFE39_00510"
     CDS             348..1613
                     /locus_tag="VFE39_00510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6257110.1"
                     /translation="MSVDTAVQPTDTGRSERAVLDVVVPVYNEENDLEPSVRRLHAHL
                     SGQFPYPFRITIADNASTDATPQIASRLAAELPGVTALRLEQKGRGRALRTAWSASDA
                     QVLAYCDVDLSTDLAALLPLVAPLISGHSDLAIGTRLGRGSRVVRGAKREIISRSYNL
                     LLRGALGAGFSDAQCGFKAVRADVAHRLLPLVEDTGWFFDTELLVLAERSGLRIHEVP
                     VDWVDDPDSRVDIVATALADLKGIVRVGRALATGSLPLAELRSQLGRGDLPTPDVAGV
                     PSGLPRQLVRFAAIGVVSTLAYVVLYTLLRGAVGPFAANLVALVLTAVANTAANRRLT
                     FGVRGGAGAGTHHFQGLIVFGLGLALTTGALAALAAWAPGASGAVELLVLVLANALAT
                     VLRFVAFRSWIFRGHRARGTHAGARRTAV"
     gene            1684..3723
                     /locus_tag="VFE39_00515"
     CDS             1684..3723
                     /locus_tag="VFE39_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6257111.1"
                     /translation="MSATLAPPRAAHALATKPRRSRGELAALAVLLVGTAVLYLWNLG
                     ASGWANSYYAAAVQSMTKNWTAFFFGSVDAGNIVTVDKPPGSLWVMALSARVFGFSSW
                     SMLVPQALMGVATVALLYVAVRRVSGPVAGLLAGVAMALTPVAVLMFRFNNPDALLVL
                     LLVAAAYATLRAIENASTRWLVFAGALIGAAFLTKMMQAFVVVPALALAYLVAAPTGF
                     WRRVRQLLAAGVAIVVAPGWWVAIAELWPASSRPYIGGSQGNSVLELTFGYNGLGRIF
                     GGEGGRGGFGRGAPGGAAPAGGPGGGAPGGGLERALPGAGAHPGGGAPGAAPGGGAYG
                     GGAFPGGGFPGGGGAGRGGGGLAGGFGGQPGITRMFGDSVGTQAAWLLPSALLLLVAG
                     LWLVRRAHRTDRLRASLLLWGTWTVVTALVFSFAQGIFHPYYTVALAPGIAALVGIGG
                     MQLWSRRATWPGRVALAVVIAVAAVWAAVLLARTPDFLPWLRWVVLAVGAVAVLTLLV
                     GSRWKSWTAATALAVLLTGLAAPAAYAVQTAATPHTGSIPKAGPASGAGGGRGGLGGS
                     GREASTAPEVVALLQAAGTKWSAATIGAQGSAALALASNTTVMGIGGFSGSDPAPTLQ
                     QFQALVAAGEVHYFVAGGFHGLGGGNSPIAAWVGQSFTSTTIGGATVYDLTRPAS"
     gene            4122..4478
                     /locus_tag="VFE39_00520"
     CDS             4122..4478
                     /locus_tag="VFE39_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HET6257112.1"
                     /translation="MEVLTVPWLFLVGAILTEVAATTALKLSEGFTRLAPSIVVVVGY
                     VTAFGMLSQALLTRGMGIGVAYGVWAAAGMALVTIIGAVFLGENMTWVQVGGIAIIIA
                     GVLTLELGAHHPGAQQ"
     gene            4475..5032
                     /locus_tag="VFE39_00525"
     CDS             4475..5032
                     /locus_tag="VFE39_00525"
                     /inference="COORDINATES: protein motif:HMM:NF037009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET6257113.1"
                     /translation="MNAPEPTDGRRRKGERRRRLLIEATMRVIERDGMGAVSQRGVAQ
                     EAGVPPSTVTYYFPAVDDLLVAALTACNDDYLERLAECAAAPDPLAQVARLISESTGA
                     RRVHVAAEYELFLMAGRRPALRPEAARWADALDAFLAPHVADPVERAGVTAAVDGLFL
                     RCFCAPDPPGAHEVHAVLARLIRSG"
     gene            complement(5066..5440)
                     /locus_tag="VFE39_00530"
     CDS             complement(5066..5440)
                     /locus_tag="VFE39_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6257114.1"
                     /translation="MVGPEQACPIAPVVDIVFSRWTTPILWALNEYGRQRFMELEHRI
                     ATITPKVLTQRLRQLERDGLVVRTYHPEVPPRVEYEISELGRSLAPLFATLAEWSAVN
                     LDKVDLARRDYDTTERPPRHRV"
     gene            5570..6415
                     /locus_tag="VFE39_00535"
     CDS             5570..6415
                     /locus_tag="VFE39_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET6257115.1"
                     /translation="MIVVTGATGNVGRPLVQALAAAGEQVTAVSRGASGAAVAEGVRH
                     RQADLAAPETLRPVFDGADALFLHDTGAGAHLLSPRDILDIAKAGGVGRVVLLSSQGV
                     ATRPQSSSHGGVGRSIEDAVRQAGMDWTILRPGGFDSNAYAWVESVRARRTIAAPFGD
                     VGLPTVDPADIAEVAAAALREDGHAGQVYELTGPALSTPRKRAEAIGDALGEPIRFIE
                     QTRDEAHAQMLQFMPEPVVETTLAILGEPTPAEQRISPDVEQVLGRPPRTFAEWAVRN
                     IAAFE"
     gene            6645..7160
                     /gene="msrA"
                     /locus_tag="VFE39_00540"
     CDS             6645..7160
                     /gene="msrA"
                     /locus_tag="VFE39_00540"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET6257116.1"
                     /translation="MTTETAILAGGCFWGAQELLRKRPGVISTRVGYSGGDTPNATYR
                     NHGDHAEAVEIVFDPDVIPFRDVLEFFFQIHDPSTENRQGNDVGRSYRSAIFYTSEEQ
                     ERVALDTIADVDASELWPGKVVTEVTAAGPFWPAEEEHQDYLQKYPAGYTCHFVRPGW
                     RLPHRQAQSAR"
     gene            7674..8126
                     /locus_tag="VFE39_00545"
     CDS             7674..8126
                     /locus_tag="VFE39_00545"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET6257117.1"
                     /translation="MTAQTPVETRTQVHVTGRRIVATLIDTALLSGLYVLTAIAFGSI
                     IDLGSPQWVVPAFATSATVVFVLVALLYYILLEGYLGRTLGKMLAGIRVVGETSGAAP
                     GPAAATVRTLLRFVDGLGFYLVAFIVVLASPRRQRLGDMAAHTLVVRS"
     gene            8168..8455
                     /locus_tag="VFE39_00550"
     CDS             8168..8455
                     /locus_tag="VFE39_00550"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HET6257118.1"
                     /translation="MEVRVLNDGERHRLEALERQMGLEEPGLAAALRHRDASANGAMG
                     WRAIALLVFAPFVFALLIVAFGVLATIITVVAVFAIAAASRRRQHNRGRGG"
     gene            complement(8520..8717)
                     /locus_tag="VFE39_00555"
     CDS             complement(8520..8717)
                     /locus_tag="VFE39_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257119.1"
                     /translation="MLTPEDLAHFEESRSRAYELLTDAAAELSPGSGLGSATGTVAAA
                     ARAEALKHIAAAQDALDRMSG"
     gene            complement(8856..9038)
                     /locus_tag="VFE39_00560"
     CDS             complement(8856..9038)
                     /locus_tag="VFE39_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET6257120.1"
                     /translation="MELLLHQHGQIHDLFAEVRASEADARRHVFGRLVRLLAMHETAE
                     EMIVHPVARTVLHQAA"
     gene            9396..9668
                     /locus_tag="VFE39_00565"
     CDS             9396..9668
                     /locus_tag="VFE39_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257121.1"
                     /translation="MKKIRHHRIVLEVAGECSGEPAARLERALRRYCELRIQGAERQS
                     EVIWRSGMPSLVSGSLLFIVGVVLSFEFTRPGAGLPRPAEVGTAER"
     gene            9710..10021
                     /locus_tag="VFE39_00570"
                     /pseudo
     CDS             9710..10021
                     /locus_tag="VFE39_00570"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
     gene            10298..13711
                     /locus_tag="VFE39_00575"
     CDS             10298..13711
                     /locus_tag="VFE39_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6257122.1"
                     /translation="MTWRRLAEPASVAAVAVAAVAAIAETLAAVVVGRVADQPTAWLV
                     GLLACLLFGSTLLDTAGRTAFSGVVGRAEGRLRADLLHAALHQPLPALEELAVGEVLD
                     RVDDDARQVGVLLRRTGWQLGRAALRSVLAWVIAGFAWWPAWIGFPLVGLLAVAVVRS
                     LTPIVAERKLAEEVAWSDHSAQLEEAVAGRDDVRSSLGQPHVVRQYTRLAQQVLRRVR
                     ATCSASTQVGLRTGLVVHAALAAIAVSGAALVGAGHLGVAGLVTLWLLVTTFAGQLSQ
                     ITNHLPEVQAGLGALQRIHGLLSAPQEPAGGAPVPSGPAAVELRNLTVGYPGGFALRS
                     VDLTVAAGTTCALVGRTGAGKSTLAKLLSRALEPPPCTVFVAGQDVTATSVEHLRRAV
                     GVVTQRTEILAATLEKNITLFADVPPAGVKDALEALGLTEWVDSLPHGLETCLGPGGS
                     TLSAGEEQLVAFARLLVRDVAVVVLDEATARMDPQTERLVTRAADRLLTGRTGIVIAH
                     RMSTTRRCDSVAVLDRGRVVQQGPRARLAAEPGPFRELLAAAGEAAPTLDAGVLVSRG
                     ERREPRPTPAPVRPRLARTVLRMLLAHPRWGVVGALGFLASSLLGAYGAVTGWPWGLV
                     VTALEGGTTPWAEAGALTGSLLLAPVAIALAFRTYPLWWSAVTLRLRLTVLRGQTMQR
                     RLVRTPPGEVVARALDSDRLVIYADRWVDVVNAMVIVLVTAVVGRSLPAGAVIGGILV
                     VSAATSALGAPLAGAAGREAGDARARFGTSLVSVLDAARTVKLAAATSAVQAHLAQVD
                     AGRVTAVVREHRVRTLLDGVPGLLVQLGVVVAWTAHLTGVWDLATALLVSTAVAGSGW
                     YGTVFGAAVTEAPVARRWLQAVSDLSGGADLVAVPPGVDLVTGTAPAPAAVGRVPLKR
                     LSLEGVTAVHDDGTVGTDGVTLDIDAGSLVLLTGRVGSGKSSLLASLAGLVDHEGSIR
                     WNDVEIDDPQLFLRPGQVAYVGQVPRVLSGSFVDNIALDHDRRLHEAVADARMQADVE
                     EAGGHGALVGHRGVRLSGGQVQRLALARALATEAELLVADDVSSALDARTEVELWEAL
                     RRRGVTVLGCSSKQSALSRADFVVVLEEGRVAASGPWSQLSDRWAHLAG"
     gene            complement(13798..14412)
                     /locus_tag="VFE39_00580"
     CDS             complement(13798..14412)
                     /locus_tag="VFE39_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6257123.1"
                     /translation="MVRKRLTREESRAQTRERLLEAARELFTERGVNGSSVEQIAERA
                     GYTRGAFYGNFTDKQELVAELLEQRTRSEVAEVSALQREAVSFGDMVEALRQWNRERG
                     EHLPEWMALRLELVLHALRNPDALPTLAQRETLARSTIQTAIEQRLAEHGGTPPADPA
                     HLALIVHALEDGLLIQRLLFPDEIADDVVVDSVNVLLRSWMSSV"
     gene            complement(14468..>14679)
                     /locus_tag="VFE39_00585"
     CDS             complement(14468..>14679)
                     /locus_tag="VFE39_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257124.1"
                     /translation="IRAMRLCTATTFVGVPAVAVPTGVADGQPLGVQVIGPQYREDLC
                     FQAAAALESRFGTLAPMEPAVDAAA"
BASE COUNT         1919 a         5169 c         5278 g         2313 t
ORIGIN      
        1 ggctaccagc tggcgaccgg caattcggtg atgcccgtcg gcggctacaa cggcaccgac
       61 ccctccccga cgctccagca gttccagcag ctcgtcgcgg aacggcagat ccactggttc
      121 atcgtcggcg gcatgatggg cggcccctcg gcgagcggca gcgaccagtc ccagcagatc
      181 tccgcctggg tggccgccac attcacgccg gtcaccgtcg acggcgtcac gctctacgac
      241 ctctcggcgg cgtcgtgacc accctcacgc ccgaccacga gcgacgcaca gctagtccac
      301 acgatcgcca caacggtggg acagcgggtg ccgagaccgt tgaaactatg agcgtcgaca
      361 ctgcggtgca acccaccgac accggacggt ccgagcgcgc ggtgctcgac gtcgtcgttc
      421 ccgtctacaa cgaggagaac gacctcgagc cgtcggtccg gcggttgcac gctcacctga
      481 gcgggcagtt cccttacccg ttccggatca cgatcgcgga caacgcgagc accgatgcga
      541 caccccagat cgcctcgcga ctggcggcgg agctgcccgg cgtcacggca ctgcggctgg
      601 agcagaaggg ccgcgggcgg gcgctgcgca ccgcatggtc ggcgtcggac gcgcaggtgc
      661 tggcctactg cgacgtggac ctgtccaccg acctggccgc cctgctcccg ctcgtggccc
      721 cgctgatctc cgggcactcc gatctcgcga tcggcacacg cctcggtcgc ggctcccggg
      781 tggtgcgggg cgccaagcgg gagatcatct cccggtccta caacctgctc ctgcgcgggg
      841 cgctgggcgc gggcttctcc gacgcgcagt gcggcttcaa ggccgtgcga gccgatgtgg
      901 cccaccgcct gctcccgctg gtcgaggaca ccgggtggtt cttcgacacc gagctgctcg
      961 tgctcgccga acgctccggc ctgcggatcc acgaggtgcc cgtcgactgg gtggacgacc
     1021 cggacagccg ggtcgacatc gtcgcgacgg cgctcgccga cctcaagggc atcgtgcggg
     1081 tcggccgtgc cctcgccacc gggagcctgc ccctcgccga gctgcgcagc cagctcggcc
     1141 gcggcgacct cccgaccccg gacgtggcgg gcgtgccgag cggcctgccg cggcagctcg
     1201 tgcggtttgc ggccatcggc gtggtcagca cgctggcgta cgtggtgctg tacacgctgc
     1261 tgcgtggcgc ggtgggtccg ttcgcggcca acctcgtcgc actcgtcctg acggccgtgg
     1321 ccaacaccgc cgccaaccgg aggctgacgt tcggcgtgcg gggcggggcc ggcgcgggca
     1381 ctcaccactt ccagggcctg atcgtgttcg ggctcgggct cgccctcacc accggtgctc
     1441 tcgccgcgct cgccgcgtgg gcgcccgggg cgagcggggc tgtggagctg ctcgtgctcg
     1501 tcctcgcgaa cgcgctggcg accgtgctgc gatttgtcgc ctttcgttcg tggatcttcc
     1561 gcggccaccg cgcccggggt acccacgccg gcgcccggag gactgctgta tgagcgagct
     1621 tgcgagcgaa tcgtggagac agtgcctgcg cgaagcgccg gccgagcgca gcgaggtcgt
     1681 gcaatgagcg caacgctggc accaccgcgt gcggcgcacg ccctcgccac aaaaccacgc
     1741 cggagccggg gcgagctggc ggcgctcgca gtgctgctgg tcggcaccgc ggtgctctac
     1801 ctgtggaacc tcggggcgtc cggctgggcc aacagctact acgccgccgc cgtccagtcg
     1861 atgacgaaga actggaccgc gttcttcttc ggctccgtcg acgccggcaa catcgtcacg
     1921 gtcgacaagc cgcccgggtc gctgtgggtg atggcactgt ccgcgcgggt tttcgggttc
     1981 tcctcgtgga gcatgctcgt gccgcaggcg ctgatgggtg tcgcgacggt ggcgctgctg
     2041 tacgtcgcgg tgcggcgcgt atcggggccg gtcgccgggc tgctcgcggg cgtcgcgatg
     2101 gcgctgacgc cggtcgcggt gctgatgttc cggttcaaca acccggacgc cctgctcgtg
     2161 ctgctgctgg tcgccgcggc gtacgcgacg ctgcgcgcga tcgagaacgc ctcaacccgc
     2221 tggctcgtgt tcgccggggc gctcatcggc gccgcgttcc tgacgaagat gatgcaggcg
     2281 ttcgtcgtgg tcccggcgct cgcgctggcc tacctcgtgg cggcgccgac cgggttctgg
     2341 cggcgggtcc ggcagctgct cgctgcgggg gtcgcgatcg tggtggcgcc gggttggtgg
     2401 gtcgcgatcg ccgagctgtg gcccgcctcg agccggccgt acatcggcgg ctcgcagggg
     2461 aacagcgtgc tggagctgac cttcggctac aacggcctcg ggcggatctt cggcggtgag
     2521 gggggccgcg gggggttcgg gcgcggcgcc cccggcggtg cggccccagc aggcggtccg
     2581 ggcggcggcg cccccggcgg tgggctggag cgcgcccttc ccggggccgg tgctcacccg
     2641 ggcggcgggg cacccggcgc cgcgccgggt gggggcgcgt acggcggggg tgcattcccc
     2701 ggcggggggt tcccgggggg gggtggcgcg ggccgcgggg gcggtggcct cgcgggcggt
     2761 ttcggcgggc agcccgggat cacgcgcatg ttcggtgact cggtcggtac gcaggcggcg
     2821 tggctgctgc cctcggccct cctgctcctg gtcgccgggc tgtggctggt gcgccgcgcc
     2881 caccgcaccg accggctccg cgcctcgctc ctgctctggg gcacctggac cgtcgtcacg
     2941 gcgctggtct tcagcttcgc ccaggggatc ttccacccgt actacacggt ggcgctggcc
     3001 ccggggatcg cagcgctcgt cgggatcggc gggatgcagt tgtggagccg gcgcgcgacc
     3061 tggccgggac gggtggcgct cgcagtggtc atcgccgtgg cggcggtgtg ggcagcggtg
     3121 ctgctcgccc ggaccccgga cttcctgccc tggctgcggt gggtggtgct cgcggtcggg
     3181 gcggtcgccg tgcttaccct cctggtgggg tcgcgctgga agagctggac cgcggcgacc
     3241 gccctcgcgg tgctgctcac ggggctcgcc gcgcccgcgg cctacgccgt acagacggcg
     3301 gcgaccccgc acacgggcag cattccgaag gcgggcccgg caagcggcgc gggtggcggc
     3361 cgaggaggac tgggcggatc gggccgggag gcgagcacgg ctcccgaggt cgtcgccctc
     3421 ctgcaggcag ccggcacgaa gtggtcggcc gcgacgatcg gggcacaggg aagcgctgcg
     3481 ctcgcgctgg ccagcaacac gaccgtgatg ggcatcggcg gcttcagtgg ctcggatccg
     3541 gcgccgacgc ttcagcagtt ccaggccctc gtcgcggcgg gtgaggtgca ctacttcgtt
     3601 gctggtggct tccatggcct ggggggcggg aactcgccga tcgcggcgtg ggtggggcag
     3661 agcttcacct ccaccacgat cggcggcgcg acggtctacg acctgacgcg gcccgcctcg
     3721 tagccggggc tggcgagcca cgttcccgca tcgtgcacgg cgggaacgtg gctcctgcac
     3781 ctcagacagt gatcgtcgtg gttgtacccc gtcgatcctg aagctatctg cttcccccgc
     3841 ccgtcgatcg aggtcggtgg agggaagtcg tggacgtcga ccgccagcac ggccgttgga
     3901 tcggccggca cgggtgcgcc cagactgcag aagagccccg gaccctgctc gggtaccggg
     3961 gctccgaaca atgcacctcc gatgtctccc tcaagggagg tgtgatcacc gtggaccgcg
     4021 cggtgagcgg cactgcgctc gtgctcagcc acagcggagc aagaaacctg agcgagcgct
     4081 ccactagttg ctcatctgga gcggtcgctc atgttgtcct cgtggaggtg ctcacagtgc
     4141 cctggctgtt cctcgtcggc gcgatcctca cggaggtcgc cgccaccacg gcgctgaagc
     4201 tctcggaggg cttcacgcgc ctcgccccct cgatcgtcgt ggtggtcggc tacgtcacgg
     4261 cgttcgggat gctgtcccag gcactactca cccgtgggat ggggatcggc gtcgcctacg
     4321 gcgtctgggc ggctgcgggc atggcgctcg tgacgatcat cggcgccgtg ttcctcggcg
     4381 agaacatgac gtgggtgcag gtcggcggca tcgcgatcat catcgccggg gtgctcaccc
     4441 tggagctcgg cgcccaccac ccgggagcac agcagtgaac gcgccggaac ccaccgacgg
     4501 ccgccgccgc aagggtgagc gcaggcgccg cctgctgatc gaggcgacga tgcgggtgat
     4561 cgagcgcgat gggatgggtg ctgtgagcca gcgcggggtc gcgcaggagg ccggcgtgcc
     4621 gcccagcacc gtcacctatt acttccccgc cgtcgacgac ctgctcgttg cggccctcac
     4681 cgcctgcaac gacgactacc tcgagcggct cgccgagtgc gcggccgccc cggacccact
     4741 ggctcaggtc gcccgtctga tctccgagag caccggcgcg cgtcgcgtcc acgtcgcggc
     4801 cgagtacgag ctgttcctca tggccggtcg gcggcccgcc ctccggccgg aggccgcccg
     4861 ctgggccgat gccctggacg cgttcctggc cccgcacgtg gccgacccgg tcgaacgcgc
     4921 cggcgtgacc gcagccgtcg acgggctctt cctgcgttgc ttctgcgctc ccgacccacc
     4981 gggcgcgcac gaggtgcatg ccgtgctcgc ccggctgatc cggtccggat aatcgtcggg
     5041 ccggtgcccg ccgcctcccg tgtggtcaca cccggtgcct cggcggtcgc tccgtggtgt
     5101 cgtaatcacg tcgggccaga tcgaccttgt ccagattgac ggcggaccac tcggccagtg
     5161 ttgcgaacag cggtgccagg ctgcgcccca gttcactgat ttcgtactcc actcgcggtg
     5221 gcacttcggg gtggtaggtg cgcacgacga ggccgtcgcg ctccagctgc cgtaaccgct
     5281 gggtcagcac cttgggcgtg atcgtggcga tgcggtgttc cagctccatg aaccgctgcc
     5341 gcccgtactc gttgagcgcc cacaggatcg gggtggtcca gcggctgaag acgatgtcaa
     5401 ccaccggcgc gatcgggcaa gcctgttcgg ggccgaccac cgcgcggtcc gtccagcctg
     5461 tgttgtcgac cacagcgaac cttcccccca ttactttctt ctaggtacct actctatcca
     5521 gcgcagtagc ttcgccgagt ccacagagaa ctcggaattg ggagcgaaca tgatcgtggt
     5581 gactggagcg accggcaacg ttggtcgtcc gctggtgcag gcgctcgcgg cagccggcga
     5641 gcaggtgaca gcggtgtccc gaggggcctc gggcgcggcc gtggcggagg gtgtccggca
     5701 ccggcaggcg gacctggccg ccccggagac ccttcggccc gttttcgacg gcgctgatgc
     5761 gctgttcctc cacgacaccg gtgccggtgc gcacctgttg agcccacggg acatcctcga
     5821 catcgcgaaa gccggcggag tcggccgggt cgtgttgttg tcctcccagg gagtcgcaac
     5881 ccgaccgcag tcgtcctccc atggaggcgt gggacggtcc atcgaggacg ccgtccggca
     5941 ggcgggcatg gactggacga tcctgcggcc tggtggcttc gactccaacg cctacgcctg
     6001 ggtcgagtcg gtccgtgccc gtcggaccat cgccgcgccg ttcggtgacg tcggcctgcc
     6061 gaccgtcgac ccggcagaca tcgccgaggt cgcagccgca gccctgcgcg aggacggtca
     6121 cgccgggcag gtctacgagt tgaccgggcc cgccctcagc acgccccgca aacgagccga
     6181 ggcgatcggc gacgcgctcg gcgagccgat ccggttcatc gagcagaccc gcgacgaggc
     6241 gcacgcacag atgctgcagt tcatgcccga gccagtggtc gagaccaccc tcgccatcct
     6301 gggcgagccc acccccgccg agcagcggat cagccccgac gtcgagcagg tcctcggccg
     6361 cccgccccgc accttcgccg agtgggcggt gcgcaacatc gccgccttcg agtgacctca
     6421 gcacacctga cgccgatcca cgtatcacac ccacgtatca ctccgccgcc cggctggact
     6481 ccggcatggc ctgcgcccac gcctcctgct gctggacgtg atcccgccga ttccctgcct
     6541 ccatgtgccg accctgggag actggaacat cggctgagac cggagtgttt gtggacgacg
     6601 tcagcgacca gcacgcatct gttcgagacc gaggaggcca cacgatgaca actgagacag
     6661 cgatcctcgc aggcggctgc ttctgggggg cacaggagtt gctacgcaag cgtccggggg
     6721 tgatctcgac ccgcgtcgga tactccgggg gagacactcc gaacgccacc taccgcaacc
     6781 acggcgacca cgccgaggca gtcgagatcg tcttcgatcc cgacgtcatc ccgttccggg
     6841 atgtgctgga gttcttcttc cagatccacg acccatccac ggagaaccgc cagggcaatg
     6901 acgtgggccg gagctaccgc tccgcgatct tttacacgag cgaggagcag gagcgcgtcg
     6961 ctctcgacac gattgcggat gtggacgcct cggagctgtg gccgggcaag gtcgtcaccg
     7021 aggtgaccgc ggccgggccg ttctggccgg ccgaggagga gcatcaggac tacctgcaga
     7081 agtaccccgc cggctacacc tgccactttg ttcggcccgg ctggagactc ccgcaccgcc
     7141 aggcgcaatc ggcgcgataa aggagaccgt cgcatcccgg cggtctcacc tggtgagctc
     7201 caacgcctcc gcgtggccag ccagctgcgc gccgggctgt ttggcgtcct ctacgtgctg
     7261 gacgagccgt ccgccggcct gcgtgcgtgt gagggaagag ttgtcggtga tccgcgcgca
     7321 caaagacgac gatcggccag cccgaccgcg accgcgactg gcggctgccc cgcctcttcc
     7381 aacgccacat aacagcgacg gccggtgatc acagcccgac ggccgcttgc cgtcctcagc
     7441 aggcgtatcg gggctggggt tggctcacgc tctgcccctg acgggtcaaa gttgcatctg
     7501 aaccttgggg ttctcaggtg gaggcgcgcg gcggcctgcc cgggcacggt ggttcatgcg
     7561 gtccggatca ccggcggcat gccgtgtgac cgaccacgcc ggagcgcgct gttcgggctc
     7621 gaccgacaaa gggcgagggc agaagcgcgg gtgctgggag agggaagggc gagatgaccg
     7681 cgcagacacc cgtggagacg aggactcagg ttcatgtcac cggccggagg atcgtcgcga
     7741 ctctgatcga caccgcgctg ctcagtggac tgtacgtgct gacggccatc gccttcgggt
     7801 cgatcatcga tctcggctca ccgcagtggg tcgtccccgc gttcgcgacg tcggcgaccg
     7861 tggtcttcgt cctcgtcgct ctcctctact acatcctctt ggagggctac ctggggcgga
     7921 ccctggggaa gatgctggcc gggatcaggg tcgtcggtga gacgagtggc gccgcccccg
     7981 ggccggctgc cgccacggtg cgcacgctgc tgcggttcgt ggacggcctc ggtttttatc
     8041 tggtggcctt catcgtggtg ctggcctcac cccgccggca acgcctcggc gacatggccg
     8101 cccacacgct cgtggtccgc agctgaggac cgggcccgca cggaggcgga gcagggttcc
     8161 tatctccgtg gaggtacgtg tgctcaacga cggcgagcgc caccggctcg aggcgctcga
     8221 gcgccagatg gggctcgagg agccgggcct ggccgccgcc ctgcgccacc gtgacgcctc
     8281 ggccaacggc gccatgggct ggcgggcgat cgcgttgctc gtgttcgcac cgttcgtgtt
     8341 cgcactgctc atcgtggcct tcggcgtcct cgccaccatc atcaccgtgg tcgcggtctt
     8401 cgcgatcgcc gctgcgtcac ggcgccgcca acacaaccgc ggccgcggcg gctgaacggc
     8461 cgggcgtgtt ccgccgtctg tgctgagggt gagcctcggg gcgaacagga cttcagtgtt
     8521 cagcccgaca ttcggtcgag cgcgtcctgg gcggccgcga tgtgcttgag ggcttcggca
     8581 cgcgcggccg cggcgaccgt gccggtagcg ctgcccagcc cactccccgg gctcagctcg
     8641 gcagcggcat cggtcaggag ctcatatgcc cgactacgtg attcctcgaa gtgggccaag
     8701 tcttccgggg tcaacatctg cccttcctct ttcaagcgcg accggggtta cccactgcca
     8761 aagccctgaa ccgccggcct gtatgcggac ccgcggatag gtgatctggc ggttccgccc
     8821 tggccagctg cgctagcgtc gccgggtcca tcacctcacg cagcttgatg aagcacggtc
     8881 cgcgccaccg gatggacgat catctcctcg gcggtctcgt gcatcgccaa caaacgcacg
     8941 agccggccga acacatgccg ccgcgcgtcg gcctcggacg cgcgcacctc cgcgaacagg
     9001 tcgtggatct gtccatgctg atgcagcagc aactccacaa cgccgtcctg caccaccgtg
     9061 gcttggtccg cctgctggtg ttgctcacac taggagcgcg cccgccagga ccagctgacc
     9121 gaacggcccc ggcgacggta gggggctggc gatggatgct cgtgagcctc gcgggtcgaa
     9181 cgcggcagcg gccactcgtg ccgcggaccg acgaccacac ccgcctggcg ccggtggcgg
     9241 tactccagag gggcagcagg atggggtcga cctctggtgg agtaggtccc gggcgcgctc
     9301 ggcgaattgt cgcctcacga ggtgtggccc ctcgcctggc gagttacccc gcgcgttccg
     9361 tacgtcccgc cctcatggtg gaccggtttc tcgccatgaa gaagatccgc caccaccgca
     9421 tcgtgctcga ggtcgcgggc gaatgctccg gcgagccggc agcgcgcctg gagcgggccc
     9481 ttcgccgcta ctgcgaactg cggatccagg gggcagaacg acagagcgag gtgatctggc
     9541 gaagcggcat gccgtcgctg gtgagcgggt cgcttctgtt cattgtgggt gtcgtgctgt
     9601 ccttcgaatt cacccggcca ggggcagggc tgccgcgtcc tgcggaggtt gggacagccg
     9661 agcgttgaca ctccggtggt gcgataagct gtagacgttc aacgatggcg attcggacgc
     9721 cctcgatcgt gtgtacgagc ccgagggcgt gttggtacct cgctcgggct atccggtgac
     9781 aggttctgca cggtcggccg cgaacgccta cctgctcggg tttggcctgc ccatgcgtgc
     9841 acatctgagg catgtctacg tcgctggaga catcgcactg ctcatcgtcg actggtccct
     9901 tcacggcacg gcacgcgacg gcagcgcggt cgatctccgc ggcacggcga gcgatgtcac
     9961 ccgttgcggc agggacggtg tctggcgcta cgtcatcgac aatccgtccg gaaccgcatg
    10021 accgtcctcc ttgctgcgat cggcacccct tcgccgcgcg aggggcgccg aggtacggct
    10081 caccggtgat cgcggtcggc gggtgacagt gatcgcggtg aaccgcactc cggcgccccg
    10141 gccagggcgc tgatgtgcgg ctcgggccaa tcacgaacgg cggaaaaccc gaggcggaca
    10201 gcggtgctcg gacgctagtt tccctgatca gcgcacatca tcaacggtct cggtagccgg
    10261 gcgggtgcgg ggacggggga acgtgcgcgc tcagccgatg acctggcgac gcctggccga
    10321 gccagcctcg gtcgccgccg ttgccgtggc cgccgtcgcc gcgatcgccg agacactggc
    10381 cgccgtggtg gtcggccggg tcgccgacca acctaccgcc tggctggtcg ggctgctcgc
    10441 ctgcctgttg ttcggatcca ccctgctcga caccgccggg cgcaccgcct tctcgggggt
    10501 cgtcggccgc gccgagggcc ggctgcgcgc cgacctgctg cacgccgccc tgcatcagcc
    10561 gctgccggcg ctcgaggagt tggcggtggg cgaggtgctc gaccgcgttg acgacgacgc
    10621 ccgccaggtc ggtgtcctgc tgcggcgcac tggatggcag ctgggccgcg ctgcgctgcg
    10681 ctcggtactg gcctgggtga tcgccgggtt tgcgtggtgg ccggcctgga tcgggttccc
    10741 gctggtgggg ctgctcgccg tggccgtggt gcgctcgctc accccgatcg tggccgagcg
    10801 caagctcgcg gaggaagtcg catggtccga ccactccgcc cagctcgagg aggccgtcgc
    10861 ggggcgtgac gacgtgcgct cctccctcgg ccagccgcac gtcgtacgcc agtacacgcg
    10921 actggcgcag caggttctgc gtcgcgtccg cgccacctgc tccgcctcca cgcaggtcgg
    10981 cctgcgcacc ggcctggtgg tgcacgcggc gctcgccgcg atcgcggtga gcggcgccgc
    11041 gctggtcggc gcgggccacc tcggggtggc cgggctggtc accctgtggt tgctggtcac
    11101 gacattcgcc gggcagctca gccagatcac caaccacctg ccggaggtgc aggccggcct
    11161 gggcgcgctg caaaggatcc acggcctgct gtccgcgccg caggagccgg ccggcggcgc
    11221 gcccgtaccg tcggggccgg ccgccgtcga gctccggaac ctgaccgtgg gttatccggg
    11281 cggcttcgcc ctgcggtctg tggacctcac agtggcggcg ggaacgacgt gcgcgctggt
    11341 ggggcgcacc ggcgccggca agtcgacgct ggccaaactg ctgtcgcggg cgctggagcc
    11401 accgccctgc accgtgttcg tcgccggcca ggacgtcacg gccacttcgg tcgagcacct
    11461 gcgtcgcgcg gtgggcgtgg tcacgcagcg caccgagatt ctcgccgcga ccctcgagaa
    11521 gaacatcacc ctgttcgccg acgtcccgcc cgcgggcgtc aaagacgccc tcgaggccct
    11581 tgggctgacc gagtgggtgg actccctgcc gcacggtctc gagacgtgcc tgggtcccgg
    11641 cggcagcacg ctgtcggcgg gggaggagca gctcgtcgcg ttcgcccggc tgctggtccg
    11701 cgacgttgcg gtcgtcgtac tggacgaggc caccgcgcgg atggacccgc agaccgagcg
    11761 ccttgtcacc cgggctgccg accggttgct caccgggcgc accggcatcg tcatcgcaca
    11821 ccggatgtcc accacgcgcc gatgcgactc cgtggcggtg ctcgaccggg ggcgcgtcgt
    11881 acagcagggc ccgcgcgccc ggctggccgc cgagcccggg cccttccgcg agctgctggc
    11941 cgccgccggg gaggccgcgc cgaccctgga cgccggggtg ctggtcagca ggggcgagcg
    12001 ccgggagccc cggccgacac ccgcccccgt ccggccccgc ctcgcccgga cggtgctgag
    12061 gatgctgctc gcccacccgc gctggggtgt ggtcggagcg ctcgggttcc tcgcctcgtc
    12121 cctgctgggc gcctacggcg cggtcaccgg atggccgtgg ggcctggtgg tcaccgccct
    12181 cgagggcgga accacgccat gggccgaggc cggcgccctc acggggtcgc tcctgttggc
    12241 ccccgtcgcc atcgccctgg ccttccgcac ctacccgctg tggtggtcgg cggtgacgtt
    12301 gcgcctgcgt ctcaccgtgc tgcgcgggca gacgatgcaa cgtcgtctcg tccgtacgcc
    12361 acccggcgag gtcgtcgccc gcgccctcga ctcggaccgg ctggtgatct acgccgaccg
    12421 gtgggtagat gtggtcaacg cgatggtcat cgtgctggtc acggccgtcg tggggcgcag
    12481 tctgccggcc ggagcggtga tcggcggcat cctggtcgtg tcagctgcga cctcggcgct
    12541 gggcgctccc ctcgccgggg cggcgggccg tgaggccggc gacgcccgcg ctcggttcgg
    12601 cacctcgctg gtctcggttc tggacgccgc ccgcacggtc aagctggccg cggcgacgtc
    12661 cgcggtgcag gcccacctgg cccaggtcga cgccggccgg gtcacggcgg tggtgcgcga
    12721 gcaccgggta cgcacgctgc tcgacggcgt gccgggactg ctggttcagc tgggtgtggt
    12781 ggtggcatgg accgcgcacc tgaccggcgt gtgggacctc gccaccgcgc tcctggtgag
    12841 cacggctgtg gccggctcgg gctggtacgg caccgtgttc ggcgctgcgg tcaccgaggc
    12901 gcccgtggcc cggcgctggc tgcaagcggt gtcggacctg tccgggggcg ccgacctggt
    12961 cgccgtgccg ccgggggtgg acctcgtcac cggcacggcg cccgcaccgg ccgcggtcgg
    13021 ccgggtgccg ctgaagcggc tcagcctgga gggggtgacc gccgtccatg acgacggcac
    13081 tgtcgggacg gacggcgtca ccctggacat cgacgcgggc tccctcgtcc tgctgaccgg
    13141 tcgggtgggc tcggggaagt cgagcctgct cgccagcctg gctggactcg tcgatcacga
    13201 gggcagcatc cggtggaacg acgtcgagat cgacgaccca cagctcttcc tccgcccggg
    13261 ccaggtcgcc tacgtcggtc aggtgccgcg ggtgttgtcg gggtccttcg tcgacaacat
    13321 cgctctcgac cacgaccgcc ggttgcacga agccgttgca gacgcccgga tgcaggccga
    13381 cgtggaggaa gccggaggcc atggcgcctt ggtcggccac cgcggggtgc gtctctccgg
    13441 cggccaggtc cagcggctgg cactcgcccg agccctggcg accgaggccg aactcctggt
    13501 cgccgacgac gtctcctcag ccctggacgc gcgcaccgag gtggagctgt gggaggcgct
    13561 gcgccggcgg ggcgtgacgg tcctggggtg cagctccaag cagtccgcct tgtcccgcgc
    13621 cgacttcgtg gtcgtcctgg aggagggacg agtggccgcg agcgggccct ggtcccagct
    13681 gtccgaccgg tgggcccacc tggcgggctg acgcaggacc cggatgtgcg acatggacac
    13741 ctgaggtgga acgggagtgc tttcgaacag tgatacgtgt ccatgtcgcc gcaggagtca
    13801 gacgctcgac atccatgagc gcagcagaac gttgacggaa tcgaccacga cgtcgtcggc
    13861 gatctcgtca ggaaacagca ggcgctggat gagcaggccg tcctcgaggg cgtgcacgat
    13921 cagggccagg tgcgcaggat cagcaggtgg ggtgccgccg tgctcggcga gccgttgctc
    13981 gatggccgtc tggatcgtgc tgcgcgccag cgtctcgcgc tgggcgaggg tgggcagcgc
    14041 atcagggttg cgcagggcgt gtagcacgag ctcgaggcgc agggccatcc actccgggag
    14101 gtgttcgccg cgctcgcggt tccactgccg cagcgcctcc accatgtcgc cgaacgacac
    14161 agcttcccgc tgcagagcgc tgacctccgc gacctcggac cgcgtgcgtt gttcgagcag
    14221 ctcggccaca agctcctgct tgtcggtgaa gttgccgtag aaggcaccgc gggtgtagcc
    14281 ggcgcgctcc gcgatttgtt cgacggagct gccgttcacg ccacgctcgg tgaacagctc
    14341 gcgcgcggcc tcgagcaggc gctcacgagt ctgtgcccgg ctctcctcac gggtcaggcg
    14401 cttcctcacc acggccgccg aatctacagg cgtcagctga gggttggtcg cttcgtccac
    14461 ggcacgtcta cgctgccgca tcgacagccg gctccatcgg agcgagcgtc ccgaaccggg
    14521 actccagtgc cgcagcggct tggaagcaca ggtcctcccg atactgcggg ccgatcacct
    14581 gaacgcccag cggctgcccg tcggccacgc cggtcggcac ggcgacggcc ggcacgccga
    14641 cgaaggtcgt ggcggtacac aggcgcatgg cgcggatga
//
LOCUS       DASRSC010000016         4948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4917495,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000016 DASRSC010000000
VERSION     DASRSC010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4917495"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1157
                     /locus_tag="VFE39_00590"
     CDS             <1..1157
                     /locus_tag="VFE39_00590"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HET6257125.1"
                     /translation="LAMAILLNGMDAEEIARWTQAMIDSGDVLSLGAVGRPLVDKHST
                     GGVGDKITLPLAPLVAACGAAVPQLSGRGLGHTGGTLDKLESIPGWRASLSLDEIAAQ
                     LSDVGAVVCATTPTLAPADRKLYALRDVTGTVESIPLIASSIMSKKIAEGTGGLVLDV
                     KVGSGAFMKTRERARELAETMVRIGSAHGLATTALLTDMSVPLGRAVGNALEVAESVE
                     VLRGGGPADVVELTVALAREMLALAGITDVDPAEVLSSGAAYPVWERMIAAQGGDPSA
                     PLPVATHVEPVLAERSGVLTGCDAMAVGVAAWRLGAGRARKEDAVQAAAGVLLLARPG
                     DEVDAGQPLLELHTDTPDAVQGARAALADGLVISDEPAARGPLLVDEIRP"
     gene            complement(1187..1450)
                     /locus_tag="VFE39_00595"
     CDS             complement(1187..1450)
                     /locus_tag="VFE39_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257126.1"
                     /translation="MLRVDVVTGADAETVHSAFERTCTQNGGRVTDSVAAVGARVCQR
                     DGTVVEGTVSGSGRTVDVYFVNADTTTATRLTQSFDEILGAVR"
     gene            complement(1833..2897)
                     /locus_tag="VFE39_00600"
     CDS             complement(1833..2897)
                     /locus_tag="VFE39_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA containing protein"
                     /protein_id="HET6257127.1"
                     /translation="MSNDSTLSFDRMRGMLMRAAEIRDSEQQQIFDSLDEIHARLAAL
                     DALGTVRKRLTELPDRTEVSVLAERLDETVAKLDAQDTSIAAVLRAVEALPDKMAKPI
                     AQLDGRLDGMAGRLEGVSGRMDGLDDRLGGLHKRLDDLDNRLDRHEMRLDAMPSSVGT
                     PIKERIDGIERTVREQLDAAVRAMDESDEGMRNLLGDTSIGLHRRLEDLAARPAVDPT
                     EPLDGLAQRLESLATRLDAVSGRLDSLEEGLSGRLGSLGGEIEQKLGKLDAALVDRPD
                     TDSLRTLVEQANEDSERRNAGQLDEAMATFAELILGRGAAVQSPPPPPRPTQRRARKV
                     TVKSPNGASVSDTVTDDPDD"
     gene            complement(3164..3922)
                     /locus_tag="VFE39_00605"
     CDS             complement(3164..3922)
                     /locus_tag="VFE39_00605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6257128.1"
                     /translation="MIFDGDVVLVTGGSRGIGRAVALEFAAGGATVAIQYRQDRRAAE
                     EVLAALPPGPHLALQADVADAVQVQGLVQTVVDRLGRVDVLVNNAGIFVQHPVLTADY
                     ATWQEAWRQTIDTNLIGPANLVHCVVPHMVAAGGGRIVNVTSRGAFRGEPEHTAYGAS
                     KAGLNSLSQSLAQELAPQGIYVTAVAPGFVETDMTTAYLDGPGGAAIRAQSPLGRTAT
                     PQEIARVVVFLATPGTESAIGTIVDVNGASYLRS"
     gene            4126..>4948
                     /locus_tag="VFE39_00610"
     CDS             4126..>4948
                     /locus_tag="VFE39_00610"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET6257129.1"
                     /translation="MPAPLNVDSVRDAPKVLLHDHLDGGLRPATVIELADAAGYAGLP
                     SADAEQLGGWFLAASHSGSLELYLETFGHTVGVMQSADALVRVAAECAEDLATDGVVY
                     AEVRFAPELHVENGLALDAVVEAVLEGFRVGSARAAERGRRIRVGCLLTAMRHAARSR
                     EIAELAVRYRDLGVVGFDIAGAEAGFPPTRHLDAFEYLRQQNAHFTIHAGEAFGLPSI
                     WEALQWCGADRLGHGVRITDDIAVLDDGTVRLGRLAAYVRDKRIPLELCPTSNLQT"
BASE COUNT          640 a         1732 c         1845 g          731 t
ORIGIN      
        1 cgctcgcgat ggccatcctg ctcaacggca tggacgccga ggagatcgcg cgctggacgc
       61 aggcgatgat cgactccggg gacgtgctgt cgctgggggc cgtcggccga ccgctggtgg
      121 acaagcactc cacaggcggg gtaggcgaca agatcacgct cccgctggcc cctctcgtcg
      181 ccgcatgcgg cgcggccgtg ccgcagctgt cgggccgcgg gctcggccac acgggcggca
      241 ccctcgacaa gctggagtcc attccgggct ggcgggcgtc gctgtcgctg gacgagatcg
      301 cggcgcagct ctccgacgtg ggggcggtgg tgtgcgcgac cacgccgacg ctggcgcctg
      361 ccgaccgcaa gctctatgcg ctgcgcgacg tcaccggcac cgtcgagtcg atcccgctga
      421 tcgcgagctc gatcatgagc aagaagatcg ccgagggcac gggtgggctg gtgctcgacg
      481 tcaaggtcgg gtccggggcg ttcatgaaga ctcgggaacg ggcgcgcgag ctcgcggaga
      541 cgatggtgcg gatcggcagc gcacacggac tggccaccac cgcactgctc accgacatgt
      601 cggtgccgct cgggcgggcg gtcggcaacg cgctcgaggt ggccgagtcg gtggaggtgc
      661 tgcgcggagg aggcccggcc gacgtcgtcg agctgaccgt ggcgctcgcc cgggagatgc
      721 tggcactggc cggcatcacc gacgtcgacc cggccgaggt gctctcctcc ggtgccgcct
      781 accccgtgtg ggagcggatg atcgcggcgc agggcggcga cccgtcggcg cccctaccgg
      841 tggcgacgca cgtcgaaccg gtgctcgccg agcgttcggg cgtgctgacc gggtgcgacg
      901 ccatggccgt cggggtggcg gcgtggcggc tgggggccgg gcgcgcccgc aaggaggacg
      961 cggtgcaggc cgcggccggg gtgctcctgc tcgctcggcc cggggacgag gtcgacgcgg
     1021 gccagccgct gctggagttg cacaccgaca cgcccgacgc ggtgcaaggc gcgcgtgccg
     1081 ccctggccga cgggctggtg atctccgacg agccggccgc tcgcggcccc ctgctcgtgg
     1141 acgagatccg cccctaggag acagtgcgct ccgagggagt acgccgttac cgcaccgcgc
     1201 cgaggatctc gtcgaacgac tgcgtgagcc tcgtcgcggt cgtggtgtcg gcgttgacga
     1261 agtacacgtc gaccgtccgc ccgctgccgg agacggtgcc ctcgaccacc gtcccgtcgc
     1321 gctgacagac ccgcgcgccc actgcggcca ccgagtcggt cactctccca ccgttctgcg
     1381 tgcacgtgcg ctcgaacgca ctgtggaccg tctccgcatc cgccccggtg accacgtcca
     1441 cccgcagcac cagcggatca ccaccgctct gcgggtggtc gggcgccgtg aagaggcacg
     1501 tcgtccctgt gaccgcgtcc tgggtctcca ggggatggtc cgttctcgtg tcgctgtgat
     1561 cccaggacag ggaggtggcg ctcgagagat catccgcgct gagcgggcag cccgcaccag
     1621 cagaatccgc ggccggcgcc gaggccgcgg gcgggaccga tgccgggccg gcgccctctg
     1681 gctgctgcgc cgccgagccg caagctgtga cggccgcgca gacagcagcg atcgccagcg
     1741 atcctcgagt tcgaggcatg gttccccatc cgaagggtgc gggtggcgga gtgtcccatc
     1801 ccggcgaccg tgctggcagg gtggagcgct ggtcagtcgt cggggtcgtc agtgacggtg
     1861 tcgctgaccg atgccccgtt gggtgacttc actgtcacct tgcgtgcccg acgctgggtc
     1921 gggcgcggcg ggggaggggg cgactggacg gcggcaccgc ggcccaggat cagctccgcg
     1981 aaggtggcca tggcctcatc gagctgaccg gcgttgcgcc gctcggagtc ctcgttggcc
     2041 tgctccacca gggtcctcag cgagtcggtg tcgggccggt ccaccagcgc ggcgtcgagc
     2101 ttgcccaact tctgctcgat ctcgccgccc agcgagccga ggcgcccgga cagtccctcc
     2161 tcgagggagt ccaaccggcc actgacggcg tccagccgcg tggccagcga ctccaaccgc
     2221 tgcgcgaggc cgtccagcgg ctccgtcggg tcgacggcag ggcgagcggc caggtcctcg
     2281 agccgccggt gcagcccgat gctggtgtcg ccgagcaggt tgcgcatgcc ctcgtccgac
     2341 tcgtccatgg ctcggacggc cgcatcgagc tgctcgcgga ccgtgcgctc gatgccgtcg
     2401 atccgctcct tgatcggggt gccgaccgac gacggcatgg cgtcgagccg catctcgtgg
     2461 cggtcgaggc ggttgtcgag gtcgtcgagg cgcttgtgca ggccgccgag ccggtcgtcg
     2521 agcccgtcca tccggcccga gacgccctcc agccgcccgg ccatgccgtc cagccgcccg
     2581 tcgagctggg cgatcggctt ggccatcttg tccggcaacg cctcgacggc ccgcaggacg
     2641 gccgcgatgg aggtgtcctg ggcgtcgagc ttggcgaccg tctcgtcgag gcgctcggcc
     2701 agcacgctga cctcggtgcg gtcgggcagc tcggtgagcc gcttgcggac cgtgccgagc
     2761 gcatcgagcg cggcgagccg ggcatggatc tcgtcgagcg agtcgaagat ctgctgctgc
     2821 tcgctgtcgc ggatctccgc ggcccgcatc agcatgccgc gcatacggtc gaaagagagc
     2881 gtggagtcat tgctcatggc ggcggggagc ttcctttgag ccggacaacg ggaagtgccc
     2941 acgcagcgta gcgagccgta tcgctcagcc gaccgcggct ccccgttcgg gggtgtttag
     3001 cctgattgga gcagcgcgcg gtccggctgc gtgaccgagc gcagccgtgc aactgcctac
     3061 gccggggagg cgtccgtcgt cgagcgtggg gccggccaca gcgtcgcggc cggcccctgc
     3121 cacggcttgc ccggcggtca gcacgccgtt cgcggcggtg ggttcagctt cggaggtacg
     3181 acgcgccgtt gacgtcgacg atcgtgccga tggccgactc cgtcccgggg gtggcgagaa
     3241 agacgacgac gcgggcgatc tcttgcggcg tcgcggtccg tccgagcggg ctctgcgcgc
     3301 ggatcgcggc gccgccgggg ccgtcgaggt aagccgtcgt catgtcggtc tcgacgaacc
     3361 cgggcgccac tgcggtgacg tagatgccct gcggtgccag ctcctgcgcc agcgactggc
     3421 tgaggctgtt cagcccggcc ttgctcgccc cgtacgccgt gtgctccggt tcgccgcgaa
     3481 aggcgccccg ggaggtgacg ttcacgatcc gtccgccgcc ggccgcgacc atgtggggca
     3541 ccacgcagtg cacgaggttc gccggcccga tcaggttggt gtcgatcgtc tgccgccatg
     3601 cctcctgcca cgtcgcgtag tccgcggtca ggaccgggtg ctgcacgaag atgccggcgt
     3661 tgttgacgag cacgtcgacc cgcccgaggc ggtcgaccac cgtctgcacg aggccctgca
     3721 cctgcaccgc gtcggccacg tcggcctgga gcgcgagatg gggaccaggc gggagcgccg
     3781 cgagcacctc ctcggccgcg cggcggtcct gccggtactg gatcgcaacc gtcgcgcccc
     3841 cggctgcgaa ctccagggcc accgcccgac cgataccgcg tgagccgccc gtgacaagca
     3901 cgacgtcgcc atcgaagatc atatgatcag catgtcagcc cgggttacgg cggggagagc
     3961 cggtcgtccg gcagcgtccg caggcgaccc gacgagctcg gcggcgcgct ccgcgatcgc
     4021 gtagaccgcc acgtcggtgt tgccgggggg atggagagga tgaccgagcc gtcggcaccc
     4081 ggtgttgttc gcttccgacg gccagttgtg ttctagcgtg tcggggtgcc cgcgccgctg
     4141 aacgtcgact ctgtccggga cgcaccgaag gtgctgctcc acgaccacct cgacggcggg
     4201 ctgcggcctg ccaccgtcat cgagctcgcc gatgccgcgg gctacgcggg gctccccagc
     4261 gccgatgccg agcagttggg cggatggttc ctggccgcct cgcactccgg ttccctcgag
     4321 ctctacctgg agacgttcgg gcacacggtc ggggtgatgc agagcgccga cgcgctggtc
     4381 cgggtggctg cggagtgcgc cgaggaccta gccaccgacg gcgtggtcta cgccgaggtg
     4441 cggttcgccc ccgagttgca cgtggagaac gggctcgcgc tcgacgccgt ggtggaggcc
     4501 gtgctcgagg gcttccgcgt cggctcggcc cgggcggccg agcgcggcag gaggattcgc
     4561 gtcggctgcc tgctcaccgc gatgcggcac gccgcgcggt cacgggagat cgcggagctg
     4621 gcggtgcgct accgggacct cggggtggtc gggttcgaca tcgccggtgc cgaggccggt
     4681 ttcccgccca cccgccacct ggacgcgttc gagtacctgc gccagcagaa cgcgcacttc
     4741 accatccacg cgggcgaggc gttcgggctc ccgtcgatct gggaggccct gcagtggtgc
     4801 ggcgccgacc ggctcgggca cggggtgcgg atcaccgacg acatcgccgt gctcgacgac
     4861 gggacggtgc ggctgggccg gctggccgcc tatgtccggg acaagcggat cccgctcgag
     4921 ctgtgcccga cgtcgaacct gcagaccg
//
LOCUS       DASRSC010000017        10487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2460952,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000017 DASRSC010000000
VERSION     DASRSC010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10487
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2460952"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFE39_00615"
     CDS             complement(<1..314)
                     /locus_tag="VFE39_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6257130.1"
                     /translation="MTSAAEQTTNGATTPARTVLELDGVTMRFGGVVALRDARLQVRE
                     SEIFALIGPNGAGKTTVFNIVTGVFHPTEGAVRFEGKQISGMKRFRITKSGIARTFQN
                     IRL"
     gene            complement(311..1459)
                     /locus_tag="VFE39_00620"
     CDS             complement(311..1459)
                     /locus_tag="VFE39_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020629775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6257131.1"
                     /translation="MNTHRKAGGTGNPLTALRAATGDVSDRVRDWWADASPWQRWGIY
                     GLVLLLAIVAPHPIIASFMSPYTDWTSLLFNPIGTYVLLAIGLNIVVGHAGLLDLGFV
                     AFYGIGAYTLAYLGTTVGWAFWPTVIMGVFLAAMSGVILGAPTLRLRGDYLAIVTLGF
                     GEIVRITAVNTKALGGARGITPIPHPPNLFGVEFLLDPLPYYYLILAVIVVAVIFSIR
                     LQRSRVGRAWTAIREDEDAAELMGVPTFRFKLLAFAIGAMVGGLAGTVFASKVIFIAP
                     TNFPFILSATILAAVLLGGAGNLPGVIIGAFLIAWLPERFRGFADYRILVFGAALVLM
                     MALRPQGLLPSRQRSAEIKEGTGGMGSLGAEVPRPDTDPDTAAVEVAR"
     gene            complement(1456..2418)
                     /locus_tag="VFE39_00625"
     CDS             complement(1456..2418)
                     /locus_tag="VFE39_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6257132.1"
                     /translation="MFNDLTQQFLPSTIGGLTIGAIYALVALGYTMVYGVLRLINFAH
                     SEIFMIGSYASLFVIISVGVTAPLTGIALLGLLILLIVVSAAVAGGSAILLEVVAYRP
                     LRRRGAPRLAALISAIGASLFLQELFALFIIPDVFGRPGRIDTAVPRIVDRDVLFTIG
                     NAQVRTDQVIVLLAAVIVMILLDQLVARTRIGRGIRATAQDPEAAVLMGVNIDTIVRL
                     TFLLGGAMAGVAGVLYAMEFENLRFNIGFILGIKAFTAAVLGGIGNIRGALLGGIALG
                     LIENWGAIFFGSQWKDVIAFVILVVVLLFRPTGILGESLQKARA"
     gene            complement(2510..3679)
                     /locus_tag="VFE39_00630"
     CDS             complement(2510..3679)
                     /locus_tag="VFE39_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HET6257133.1"
                     /translation="MSRKRMVVTAAMLAGALALTACGGGGSPSGGGGGGETLKIGFMG
                     DLTGENSGIVIPPRNGAKLAIDEYNATNPAVKLQLVEYDSQGSADQAVPLTQQAIQQD
                     KIVGLVGPAFSGESKAVGPILEQAKIPSISASATNPGLAQNGWKYWHRVVANDADQGP
                     GIVDFLVRAAAPKKAYVISDDQEYSVGLADAAFGAFQAKGVQVERDQFAQAASDYSST
                     VAKVNAFKPDVIVYGGYYSQAGRLLKQLRDAGVTATFASGDGSLDPQIIQGAGAPAAE
                     GAVLACPCNIPFGATTGALADFTARYKAAVKADPAIYSTEGYDAATLFVDAVKAGNTT
                     AEAINTYVSGAKLDGVSKKIAFKPNGEPDVNAIFIYQVKNGALTLLGQSTEAKLG"
     gene            complement(3868..4476)
                     /locus_tag="VFE39_00635"
     CDS             complement(3868..4476)
                     /locus_tag="VFE39_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6257134.1"
                     /translation="MTADETGQGIGLRVLVVEDEALIRLDLAEMLTEEGYTVAGEAGD
                     GEQAVALARELRPDLVIMDVKMPKVDGITAAASIVEERIAPVVMLTAFSQRDLIEQAR
                     DAGAMAYLVKPFARHELVPAIELAVSRFAEKRALEDEVATLTERLETRKIVDRAKGLL
                     MTRQSMSEPEAFRWIQRTAMDRRTTMKAVAEAVVDGLNPPKS"
     gene            4652..4725
                     /locus_tag="VFE39_00640"
     tRNA            4652..4725
                     /locus_tag="VFE39_00640"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4686..4688,aa:Leu,seq:caa)
     gene            5515..6183
                     /gene="nthB"
                     /locus_tag="VFE39_00645"
     CDS             5515..6183
                     /gene="nthB"
                     /locus_tag="VFE39_00645"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HET6257135.1"
                     /translation="MDGIADMGGTDGWGAVMVPARDEPVFAEPWEGRAFALALLTIGR
                     VSGRNLDAFRHALQRLDRTAYLDDGYYGRWLNAAELMLVDSAILAPGAVDARAHAMRG
                     EQVEEPAVPEPSKPDYSATGPGSLREVPEPPRFAHGDRVRAHDISPAGHTRLPRYVRG
                     HVGTVELVQPGHLLPDTHAHFQGENPQHVFTVRFDSRELWGAEAEPFVLTIELFDSYL
                     EPAS"
     gene            6180..6770
                     /gene="nthA"
                     /locus_tag="VFE39_00650"
     CDS             6180..6770
                     /gene="nthA"
                     /locus_tag="VFE39_00650"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HET6257136.1"
                     /translation="MSTSAERLPPSLRTEALEQLLTERGLVDPAVMDGFIANYEENVG
                     PLNGAKVVARAWTDPEYKKRLLADGTAAIKELGFGGPQGEHIVVVENRPTRHNVTVCT
                     LCSCYPWPVLGLPPSWYKDPAYRARVVREPRVVLSEMGLDLADDVEIVVHDSSAEVRW
                     LVLPERPAGTEQLSEEELVPLITRDALVGVAKVVAP"
     gene            6767..7141
                     /locus_tag="VFE39_00655"
     CDS             6767..7141
                     /locus_tag="VFE39_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HET6257137.1"
                     /translation="MTTAPLAYDGPAAPPRSNGELDFAQPWESRAFGMAVSLHDAGAF
                     AWPVFQSALIRRISAWEATHPDGAGWSYYQHWLGALEDVLTADGTVTSAEVDARAEEL
                     AARPAGHDHARSSTGHGHGHGH"
     gene            7215..7799
                     /locus_tag="VFE39_00660"
     CDS             7215..7799
                     /locus_tag="VFE39_00660"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET6257138.1"
                     /translation="MSTDPLAAPDTTRDASAGWKDVVGSIGPKVRALRRERGLTLQQL
                     AHAADVSTASVHKVERGDMVPTVTTLLKIAGALGVPVRLFVEDSEPSQAVHTRFGDAV
                     RAGPVVITGPPDRFRSSGTVARLEPGDERPGLRRAGEILLVVLEGRLDVEVAAARYVV
                     AAGEALHFPSWLEHRCHNSGETTAEVVAIDVPDA"
     gene            7917..8312
                     /locus_tag="VFE39_00665"
     CDS             7917..8312
                     /locus_tag="VFE39_00665"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET6257139.1"
                     /translation="MPQVLDHHGQRCRRSAALGPTGRDDRPRIEDVRQIDVREPPAAP
                     GVRHGGLVEHRPGDRAHSAAKRGISVTDLRVEVTGYDDGPRFTAFRIVVHTDVPPGAK
                     LDAVIAAARRVCYVTRTLANPPEIDVVLG"
     gene            complement(8315..9445)
                     /locus_tag="VFE39_00670"
     CDS             complement(8315..9445)
                     /locus_tag="VFE39_00670"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET6257140.1"
                     /translation="MDDRALDLLTRTPLVDGHNDLPWALRRLAGPDPADAVAGTDLLA
                     GLPALQTDLPRLRAGRVGVQFWSVYVPCSFTGADAVTAVLEQVELVHRLTERYPDDFG
                     LATTADEAEAAFAAGRVASLLGAEGGHCIDSSLGVLRALRRLGVRYLTLTHNLNTDWA
                     DSATDEPVHGGLSHFGRDVVREMNRIGMIVDLSHVAATTMRDALATTAAPVLFTHSSC
                     RTVTDHPRNVPDDVLGALAGNGGVCMVTFVPRFVSGAAAGWDTQLQAAMTDAGLDHRD
                     LRARNTFAESWDGLPCPAATLADVVTHLEHAREVAGVDHIGLGGDFDGTRELPVGLED
                     VSRYPALFAALLERGWSEDDCAKLAGRNALRVLRAADDVATA"
     gene            9504..9998
                     /locus_tag="VFE39_00675"
     CDS             9504..9998
                     /locus_tag="VFE39_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HET6257141.1"
                     /translation="MTTPRDRDAAGRARNARPRDEAGRPLPHGAQGVERVPEDLQLTG
                     DGAVTEAQRLLDAGQPFAAHEVLEGAWKAAPEPERELWRALAQLAVGLTHAQRGNARG
                     AVALLDRAAGHLATWDGAAPAALAISDVRAHAGALARRIERDGVADLADDDLRPRLRG
                     ATRG"
BASE COUNT         1602 a         3747 c         3634 g         1504 t
ORIGIN      
        1 aggcggatgt tctggaacgt gcgcgcgata cccgacttcg tgatgcggaa gcgcttcata
       61 ccgctgatct gcttgccttc gaagcgaacg gcaccctcag tggggtggaa gacgcccgtc
      121 acgatgttga agacggtggt cttccccgcc ccgttcgggc cgatcagggc gaagatctcg
      181 ctctcccgga cctgcagccg ggcgtcccgt agggcgacga cgccgccgaa ccgcatcgtg
      241 acgccgtcca gctcgaggac ggtgcgcgcc ggggtcgtcg cgccgttggt cgtctgctcg
      301 gcagcggagg tcatcgcgcc acctccaccg cggcagtgtc cggatccgtg tccggccgcg
      361 ggacttcggc acccaagctg cccatgccgc ccgtgccttc cttgatctcc gcgctgcgct
      421 gtcgcgacgg cagcagaccc tgcggccgca gcgccatcat cagcacgagc gcggcaccga
      481 acacgaggat gcggtagtcg gcgaagccgc ggaaccgctc gggcaaccac gcgatgagga
      541 acgccccgat gatcacgccg ggaaggttgc ccgccccgcc gagcagcacc gcggcgagga
      601 tcgtcgccga caggatgaac gggaagttcg tcggcgcgat gaagatgacc ttgctggcga
      661 acaccgtgcc ggccagtcca ccgaccattg cgccgatcgc gaaggcgagc agcttgaagc
      721 ggaacgtcgg gacgcccatc agctcggcag cgtcctcgtc ctcccggatc gccgtccatg
      781 cccggccgac ccggctgcgt tgcagccgta tcgagaagat gacggccacg acgatcaccg
      841 caaggatcag gtagtagtac gggaggggat cgagcaggaa ctccacgccg aacaggttcg
      901 gcgggtgcgg aatgggtgtg atgccccggg caccgcccag cgccttggtg ttgaccgcgg
      961 tgatgcggac gatctccccg aagccgaggg tgacgatcgc gaggtagtcg ccacggagcc
     1021 gcagggtcgg ggccccgagg atcaccccgg acatcgcggc caggaagacc cccatgatca
     1081 cggtgggcca gaacgcccat cccacggtgg tgccgaggta ggcgagcgtg taggcgccga
     1141 tgccgtagaa cgcgacgaaa cccaggtcga ggagcccggc atgcccgacc acgatgttga
     1201 ggccgatcgc gagcaggacg tacgtgccga tcgggttgaa cagcagcgac gtccagtcgg
     1261 tgtacgggga catgaacgag gcgatgatcg ggtgtggcgc gacgatcgcc agcagcagga
     1321 ccagcccgta gatgccccag cgctgccagg gcgaagcatc ggcccaccag tcccggacgc
     1381 ggtcggacac gtcgcccgtc gcggcccgga gcgcggtgag gggattgccg gtgccgccgg
     1441 cctttctgtg cgtgttcatg cgcgagcctt ctgcagggat tcaccgagga tgccggtcgg
     1501 ccggaacagc agcaccacga ccaggatgac gaaggcgatg acgtccttcc attgggaccc
     1561 gaagaagatc gccccccagt tctcgatcag cccgagcgcg atgccgccga gcagcgcccc
     1621 gcggatgttg ccgattccgc cgagaaccgc ggctgtgaac gccttgatgc cgaggatgaa
     1681 gccgatgttg aaccgcaggt tctcgaactc catcgcatag agcacgccgg ccacgcccgc
     1741 catcgcacca ccgagcagga acgtcaggcg gacgatggtg tcgatgttga cgcccatcag
     1801 cacggccgct tccggatcct gtgccgtggc gcggatgccg cggccgatcc gcgtgcgggc
     1861 caccagctgg tcgagcagga tcatcacgat caccgcagcg agcaggacga tgacctggtc
     1921 ggtgcggacc tgggcgttgc cgatcgtgaa cagcacgtcg cggtcgacga tgcgtgggac
     1981 cgcggtgtcg atgcggccgg gccggccgaa aacgtccggg atgatgaaca gcgcgaacag
     2041 ctcctgcagg aacagggaag cgccgatcgc ggagatgagg gcggccagcc ggggagcgcc
     2101 gcggcgtcgc aacgggcggt aggccaccac ctcgagcagt atcgccgaac cgccggcgac
     2161 cgcggcggac acgacgatca gtaggataag cagcccgagg agggcgatgc cggtcagcgg
     2221 agcggtcacg ccgaccgaga tgatgacgaa cagggacgcg tagctcccga tcatgaagat
     2281 ctcggagtgc gcgaagttga tcagtcgtag tacaccgtag accatcgtgt agcccagagc
     2341 gacgagcgcg tagatcgcgc cgatcgtcag accgccgatg gtgctcggca ggaactgctg
     2401 ggtcaagtcg ttgaacacag accttcagcc ttctcggggg cggacctcaa ccggcacacg
     2461 gcacggggag cggacgcccc tgcgtccgcc ccccgtgtcg tgagcagcgt cagcccagct
     2521 tcgcctcggt cgactggccg agcagcgtca gggcgccgtt cttcacctgg tagatgaaga
     2581 tcgcgttgac gtccggctcg ccgttcggct tgaaggcgat cttcttcgac accccgtcca
     2641 gcttggcgcc cgagacgtag gtgttgatcg cctcggcggt ggtgttgccc gccttgaccg
     2701 cgtctacgaa cagtgtggcc gcgtcgtagc cctcggtgga gtagatcgcc gggtcggcct
     2761 tcaccgccgc cttgtaccgg gcggtgaagt ccgcgagcgc gccggtggtg gcgccgaacg
     2821 ggatgttgca cgggcaggcc agcacggcgc cctcggcggc gggtgcgcca gcgccctgaa
     2881 tgatctgcgg gtcgagcgaa ccgtcgccgc tggcgaaggt ggcggtgacg ccggcgtcgc
     2941 gcagctgctt gagcaggcgg cccgcctggg agtagtaccc gccgtagacg atcacgtccg
     3001 gcttgaatgc gttgaccttg gcgaccgtgg acgagtagtc cgaggctgcc tgggcgaact
     3061 ggtcgcgctc gacctgcacg cccttggcct ggaaggcccc gaacgccgca tcggccagac
     3121 cgacgctgta ctcctggtcg tcgctgatca cgtaggcctt cttcggggcc gcggccctga
     3181 cgaggaagtc gacgatcccg gggccctggt cggcgtcgtt cgcgaccacg cggtgccagt
     3241 acttccaacc gttctgcgcc agacccgggt tcgtcgccga ggccgagatg ctgggaatct
     3301 tcgcctgctc caggatcgga ccgaccgcct tggactcacc cgagaaggcg ggcccgacga
     3361 gtccgacgat cttgtcctgc tggatcgcct gctgggtgag ggggaccgcc tggtcggcgc
     3421 tgccctggct gtcgtactcg accaactgga gcttcaccgc ggggttcgtg gcgttgtact
     3481 cgtcgatcgc gagcttcgcg ccgttgcgcg gcgggatcac gatgccggag ttctcgccgg
     3541 tcaggtcacc catgaacccg atcttcagag tctcgccgcc gccacctcca cccgaggggc
     3601 ttcccccgcc gccacatgcc gtgagcgcga gcgctccggc gagcatcgcg gcggtgacga
     3661 ccatccgctt ccgactcatg atcgaatcct tccgtactgc tcgatctgcc tcaggcaacc
     3721 ccccgcgatc gccccttggc tcccgcttac cgggagactc gcggacggta agggacagcc
     3781 catgagtgcg tcaccattcc gggcggctcc tttaccggac cgcaacgtcc ggacgcggaa
     3841 ggtgactatc gagcagcaca gggtgattca ggacttgggc ggattgagcc cgtcgacgac
     3901 ggcctccgcg acggccttca tggtcgtcct gcggtccatc gcggtgcgct ggatccagcg
     3961 gaaggcctct ggctccgaca tgctctgccg ggtcatcagc agccccttgg cgcggtcgac
     4021 gatcttgcgg gtctcgaggc gttcggtgag cgtggcgacc tcgtcctcca gcgcgcgctt
     4081 ctccgcgaac ctcgagacgg ccagctcgat ggccggcacc agctcgtgcc gcgcgaacgg
     4141 cttcaccagg taggccatcg cgccggcgtc gcgcgcctgc tcgatcaggt cgcgctggct
     4201 gaacgccgtg agcatgacca caggtgcgat ccgctcctcg acgatcgacg ccgccgccgt
     4261 gatgccgtcc accttcggca tcttgacgtc catgatcacg agatcggggc gcagctcgcg
     4321 agccagcgca acggcctgct cgccgtcgcc tgcctcaccg gcgacggtgt agccctcctc
     4381 cgtcagcatc tcggcgaggt ccaggcggat cagcgcctcg tcctccacca cgagcacccg
     4441 cagcccgatg ccctgtccgg tctcgtccgc ggtcatgtcc tgctctgaaa cctgctgcgt
     4501 cacgagggct tctctccttc gccggagggt cgtaccggaa ggattcagag taccgggcgc
     4561 cacgcggtgc acggctgtga tctcggtgga cgtgggtcgg ggggtgggca ggcagcgccg
     4621 agcgcccccg ctaggatctc ctccgcaacc tgcccccgta gcccaattgg cagaggcaac
     4681 ggattcaagt cccgttcagt gtgcgttcga gtcgcaccgg gggcacagcg tcggcacgaa
     4741 cggtcgtggt gccgctcagg ccgggtggcg accgatctgt agcagcggcg ccgaggtcgc
     4801 atccggggtt gccggcccgc agagcgatgc cgaagcggcc cgctgcacga ggttcagcga
     4861 cgagcatgcc tggcatcggg cacaccctgc gacggcggtg tccgccgcga gcttgacgcc
     4921 gtcgcgatag atgctctggt cgtcgtcggc gcggagccgg tagggcgagg ttgagctcgg
     4981 aggcacgtca cggggacgcc ctcgatccac aagatcccgg agtccgacgt ccccgcccca
     5041 ccccttcggg tcgtctcgag ttccgtgtcg atcctccgac ccgccgcctg cagatccatg
     5101 atcagctcgg ccacacccgc gatgtcggtc agctcgatcc ggacggattc cgcgtcgttc
     5161 ggagcttcgg ccacggttct actatagtag aacgatgccg gtcacacacc gtggacaaac
     5221 ggagagcccg cgcaggatcg ccgtcgatgc gccactctga ggaccgccga gaggcactgc
     5281 gatgacggga gctgaccctt cccgacacga tcgcggtggt gaccatcggc ctgctgatgt
     5341 tcggcgacgc gatccgccgg tcaggtcgga gcaggggtcg ccaccgcgcc cctcgggtgc
     5401 ttcaccgccg ccctgctcga cctcgcggaa cggctcgccg actgacgagc accgccgcca
     5461 ccggccatac actatggtag aaattcttcc tgtatcgcga cgagaggagc ctccatggac
     5521 ggcatcgccg acatgggtgg aaccgacggc tggggagccg tcatggtgcc ggcgcgcgac
     5581 gagccggtct tcgccgaacc gtgggaggga cgcgccttcg cgttggcgct cctcacgatc
     5641 ggccgggtct caggccggaa cctcgacgcg ttccggcacg cgctgcaacg cctcgaccgc
     5701 accgcatacc tggacgacgg ctactacggg cgctggctca atgccgcgga gctcatgctc
     5761 gtcgacagcg cgatcctcgc ccccggcgcg gtcgacgccc gcgcccacgc aatgcgcggt
     5821 gagcaggtcg aggaacccgc ggtgcccgag ccctcgaaac ccgactactc cgccaccggc
     5881 cccggctcgc tgcgcgaggt gcccgagccg ccgcgcttcg cccacggcga ccgggtgcgg
     5941 gcccacgaca tctccccggc cggccacacg aggctgccgc ggtacgtgcg cgggcacgtc
     6001 ggcaccgtcg agctcgtgca gcccggccac ctgctgccgg atacacacgc ccacttccag
     6061 ggcgagaacc cccagcacgt cttcaccgtc cgcttcgact cccgcgagct gtggggcgcg
     6121 gaagcagagc ccttcgtcct cacgatcgag ctcttcgaca gctacctgga gcccgcctca
     6181 tgagcacatc cgccgagcgc cttcccccct cccttcgcac cgaggccctc gaacagctgc
     6241 tcaccgagcg ggggctcgtc gaccccgccg tgatggacgg cttcatcgcc aactacgagg
     6301 agaacgtcgg cccgctcaac ggtgccaagg tcgtcgcaag ggcctggacc gacccggagt
     6361 acaagaagcg cctgctcgcc gacggcaccg ccgcgatcaa ggagctgggg ttcggcgggc
     6421 cgcagggcga gcacatcgtc gtcgtggaga acaggcccac ccgccacaac gtgacggtct
     6481 gcacgctctg ctcctgctac ccctggcccg tgctggggct gccgccgtcg tggtacaagg
     6541 accccgccta ccgggcgcgc gtggtccgcg aaccgcgcgt ggtcctctcc gagatgggcc
     6601 tcgacctcgc cgacgacgtc gagatcgtcg tgcacgactc gagcgccgag gtgcgctggc
     6661 tcgtcctgcc ggaacgtccg gccgggaccg agcagctctc cgaggaggag ctggtgcctc
     6721 tcatcacccg cgacgccctg gtcggcgtcg cgaaggtcgt ggcgccgtga ccaccgcgcc
     6781 gctggcctat gacggtccgg ccgcgccccc gcggtccaac ggcgagctcg acttcgccca
     6841 gccgtgggag tcacgcgcct tcggcatggc ggtcagcctg cacgatgccg gcgccttcgc
     6901 gtggccggtg ttccagtcgg cactcatccg gcggatctcc gcatgggagg ccacgcatcc
     6961 ggacggcgcg ggctggagct actaccagca ctggctcggg gccctggagg atgtcctcac
     7021 agccgacggc accgtcacct cggcggaggt cgacgctcgg gccgaggagc tggccgcgcg
     7081 gcccgccggg cacgaccacg cgcggtcgtc cacgggccac gggcacgggc acgggcactg
     7141 acgacggaac gcactatggg aaacaaaatt ctacgatatt cacaagcaat gccctgtcaa
     7201 accgtggagg acccatgtcc accgacccgc tcgccgctcc cgacaccacg cgggacgcgt
     7261 cagccgggtg gaaggacgtg gtgggctcca tcggccccaa ggtgcgggcc ctgcgccggg
     7321 agcgcggcct gacgctccag cagctcgcgc acgcggccga cgtgtccacg gcctccgtgc
     7381 acaaggtcga gcgtggcgac atggtgccca ctgtcaccac gcttctcaag atcgccggag
     7441 cactcggggt cccggtgcgg ctcttcgtcg aggacagcga gccgagccaa gccgtgcaca
     7501 cccggttcgg cgacgcggtc cgggccgggc ccgtcgtcat caccggcccg cccgatcgtt
     7561 tccggtcgag cggcaccgtc gcgcggctcg aacccggcga cgagcgccct ggcctgcgcc
     7621 gggcgggcga gatcctgctc gtggtcctcg agggtcggct cgacgtggag gtcgccgcgg
     7681 ctcgctacgt cgtcgcggcg ggcgaggcac tgcacttccc ctcatggctc gagcatcggt
     7741 gccacaactc cggtgagacg acggcggagg tcgtcgcgat cgacgtgccg gacgcctgag
     7801 cggtcaaccg gccggttccg ccgccgccga cgcgcgcgcg ggtcaggagc ccggcctccg
     7861 gccgcggcga agcccgcggt ccgctggtcg ccggccgccc cgagctccac gagcacgtgc
     7921 cgcaggttct cgatcatcac ggccagcggt gccggcgcag cgccgccctc ggcccgacgg
     7981 gacgcgacga tcgaccgcgg atcgaggacg tccgtcagat cgacgtccgc gagcccccag
     8041 ctgcgcccgg cgtgcgccat ggcggcctcg tcgagcatcg ccccggtgat cgcgcccaca
     8101 gcgcggcaaa acgcgggatc tccgtcaccg acctgcgggt cgaggtgacc ggctacgacg
     8161 acgggccgcg gttcacggcg ttccggatcg tcgtgcacac cgacgtcccg cccggcgcga
     8221 agctcgacgc ggtgatcgcg gcggcgaggc gcgtctgcta cgtcacgcgg accctcgcga
     8281 accccccgga gatcgacgtg gtgctcggct gagctcaggc cgtcgcgacg tcatcggccg
     8341 cccgcagcac gcgcagggcg ttgcgcccgg ccagcttggc gcagtcgtcc tcgctccagc
     8401 cgcgttccag cagagcggcg aacagcgccg ggtaccggga gacgtcctcc agcccgaccg
     8461 ggagctcgcg cgtgccgtcg aagtccccgc ccaggccgat gtggtcgaca cccgccacct
     8521 cgcgggcatg ctcgaggtgc gtcacgacgt ccgcgagggt ggcagccgga cacggcaggc
     8581 cgtcccacga ctcagcgaag gtgttgcggg cccgcaggtc gcggtggtcg agcccggcgt
     8641 cggtcatggc ggcctgcagc tgcgtgtccc acccggcggc agcccctgag acgaagcgcg
     8701 ggacgaaggt caccatgcag accccgccgt tgccggcgag cgctcccagc acgtcgtccg
     8761 ggacattgcg cgggtggtcg gtcaccgtgc ggcacgacga gtgggtgaac agcaccggcg
     8821 ccgcggtggt ggcgagcgca tcgcgcatgg tcgtcgccgc cacgtgggag aggtcgacga
     8881 tcatgccgat ccggttcatc tcccgcacca cgtcgcgacc gaaatggctc aggccgccgt
     8941 gcacgggctc gtcggtggcc gagtcggccc agtcggtgtt caggttgtgg gtgagggtca
     9001 gatagcggac gcccagccgg cgtagggccc gcagcacgcc gagcgagctg tcgatgcagt
     9061 gcccgccctc cgcgccgagc agcgacgcca cccgcccggc cgcgaaagcg gcctcagctt
     9121 cgtctgcggt ggtcgcgagc ccgaagtcat ccgggtaccg ctcggtgagc cggtgcacca
     9181 gctccacctg ctccagcacc gccgtcaccg cgtcggctcc cgtgaacgag cacggcacgt
     9241 agaccgacca gaactggacg ccgacgcggc cggcacgcag tcgcggcaga tcggtctgca
     9301 gcgcgggcag cccggcgagg aggtcggttc ccgccacggc atcggcggga tcgggcccgg
     9361 cgaggcgccg cagcgcccat ggcaggtcgt tgtgcccatc gacgagtggt gtgcgggtga
     9421 gcaggtcgag cgcgcggtcg tccacggccc gagtattgcg ccccgccgca cgttgtcgat
     9481 ccgatcgtgg cacggtgcac gttgtgacca ccccgcgcga tcgcgacgcc gcaggacggg
     9541 cccgcaatgc ccgtccccgc gacgaggccg gccgccccct cccccatggc gcgcagggcg
     9601 tcgagcgggt gccagaggac ctgcagctca ccggcgacgg ggccgtgacc gaggcgcagc
     9661 gactcctcga cgccggtcag ccgttcgccg cgcacgaggt gctggagggg gcctggaagg
     9721 cagccccgga gccggagcgt gagctgtggc gcgcgctcgc ccagctcgcc gtcggcctga
     9781 cccacgccca gcgcggcaac gcccgcggtg ctgtggcgct cctcgaccgc gccgccggcc
     9841 acctcgccac ctgggacggt gcggcgcctg ccgcgctcgc gatctccgac gtccgggccc
     9901 acgccggtgc gctggcccgc cggatcgagc gcgatggcgt ggcggatctc gcggacgacg
     9961 acctgcgccc gcgcctgcgg ggcgcgacac ggggctgacg ccctccctcc gtcaacctgc
    10021 tcgccacggt cgcgcgctcc ggggagcatg cactgctgtg ccctctgacc cacccgtact
    10081 gaccgacggc gtcgtgacgc tgcgccccca caccaccgcc gacatcgacg ggttggtcgc
    10141 gatgggctcc gacccggaga tggcgcgctg gacgagcgtg ccggtgccct acgagcgcca
    10201 ccacgcggaa cgctgggtcc acgaggtggc gccgaccgga tgtcgggacg gagcggcctt
    10261 cggctgggcg atcgaggcgc tcgacggggg cacgcgccgg ttcgcgggca acgtcgacat
    10321 ccagccggta ctggctggcc accctgcccc acccgtacac ggagcagacc gctcgagagt
    10381 tcgtgcggag ccggcggctc ggcgagtcgc tcgggcagtc ggtcacctgg gtggtggccg
    10441 accgcgagga cgaccgcctg ctcgccaacg tgaccgtgtt ccggctc
//
LOCUS       DASRSC010000018         4665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2133797,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000018 DASRSC010000000
VERSION     DASRSC010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2133797"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..1986
                     /locus_tag="VFE39_00680"
     CDS             121..1986
                     /locus_tag="VFE39_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6257142.1"
                     /translation="MSEHPAAPVPDRLFDDEREQRWRARFTAPRMSRPSWARHAPDRS
                     VYTSNASGTTEVYAWDRATDTHRQVTDRRSGTHSATLPPDGETVWWFADTDGDEFGHW
                     VVEPFAGLPAGTTAEPAVSGVEDGYPAGLEIGRNVVALGTSTDGGTKIWLSRGQGPAE
                     IVYTHAEDAGVGSLSEDETLLAISHSEHGDSRHPAVRVVCVSDGAAVAEKSDGKGKGL
                     TPLAFAPVPGDGRLLLLHERRGREELLVWDVLTDTETELALDLPGEVVADFYPDGRSL
                     LVWHTHAARTRLHRYDLETGELTELPVAPGCVGSAEVRPDGTVEYTCSSAAEPSAVRA
                     LHPDGTDRVLVAPPGERAPGSVPVEDLWVDGPGGRVHALVARPAGVEGPAPTVVTLHG
                     GPHAADEDRFDPGRAAWVDAGFLVVEVNYRGSTGYGSTWRDAIEGRPGLTELEDVAAV
                     LDHCVAAGLADADRCIVEGWSWGGYLALLAVGTQPERWAAGLAGVPVADYLTAYADEM
                     EQLRAFDRALFGGSPQERPEAYRASSPLTYVNEVRTPVLVLAGENDPRCPIQQVDNYL
                     DALAARSGGGTGTGAARRTGLYEVSRFDAGHGSLVVDETLRHVATEIGFALRAVA"
     gene            2117..3154
                     /locus_tag="VFE39_00685"
     CDS             2117..3154
                     /locus_tag="VFE39_00685"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6257143.1"
                     /translation="MALWRVHVQLEDRPGRLGELATAVGAAGCNIISLHVVGEPADDG
                     SITDELLVKVPESADPATLVDAVESAGIPCTLLVRADATELSDPATTALALARMVAAD
                     PGSGPRAVATMLRARLVDPAAENHGGHVHVIRVGAQQLRLGRAWPFTATELSRAAALL
                     ELATQLAMRAPIRETSPERILLLRDGSEVRMRPAASADAPLVAALHARCAPDSRRSRF
                     LSPTRQLAPGELEALLGGASGSGQAVLAVTADGGSAVGIANLDSVWTGSARAAVLVED
                     SWQGRGLGTALLRRIADMATEQGMAELTGIARPDDLGVTRLLRRAGMRPSAEIVDGEV
                     RLRAPLPAPVA"
     gene            complement(3204..4289)
                     /gene="ugpC"
                     /locus_tag="VFE39_00690"
     CDS             complement(3204..4289)
                     /gene="ugpC"
                     /locus_tag="VFE39_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET6257144.1"
                     /translation="MAAVTYDSASRIYPGSTTPAVDSLDLEVADGEFLVLVGPSGSGK
                     STALRMLAGLEDIDKGSIHIGGRDVTRVPPKDRDIAMVFQNYALYPHMTVAENMGFAL
                     KIAGQSKVMIRERVLEAAKILDLVDYLERRPKALSGGQRQRVAMGRAIVRQPQVFCMD
                     EPLSNLDAKLRVSTRTQIASLQRRLGTTTVYVTHDQVEAMTMGDRVAVLKDGILQQVG
                     TPREMYDAPRNVFVAGFIGSPAMNLLPVDVDGDVLHFGGADIRVPRQAADAVGATRSV
                     TVGVRPEDMDIVESGAGLLTEVDVVEELGADAYVYGSTEVGGERRPVIARVDGRTPPS
                     KGAVLHLAPREGALHLFAGDTGERISL"
BASE COUNT          638 a         1668 c         1706 g          653 t
ORIGIN      
        1 cctcggctgc cgaagatggt cccgttcccc cgagtccgca ctccaggatt caaccggcgg
       61 acggtggacg gcgtggcggc gcccccggcg accggtcgcc cgtgcggcag gatctcggat
      121 gtgagcgaac accctgccgc ccccgtcccc gaccgactct tcgacgacga gcgtgagcag
      181 cggtggcggg ccaggttcac cgcgccgcgg atgtcccgac cgagctgggc gcgtcatgcc
      241 cctgaccggt cggtgtacac gtcgaacgcc agcgggacga ccgaggtcta cgcctgggac
      301 cgggcgacgg acacgcaccg gcaggtgacc gaccgtcgca gcggcacgca ttcggcgacc
      361 ctgccgccgg acggcgagac cgtgtggtgg ttcgccgaca ccgacggcga cgagttcggg
      421 cactgggtgg tcgagccgtt cgccggactc cccgccggca ccaccgccga acccgcggtc
      481 tccggggtcg aggacggcta cccggccggg ctggagatcg gccggaacgt ggtggccctc
      541 ggcacctcga ccgacggcgg cacgaagatc tggctgagcc gcgggcaggg cccggccgag
      601 atcgtctaca cgcacgcgga ggacgccggc gtcggctccc tgtccgagga cgagacgctg
      661 ctcgcgatca gccactcgga gcacggcgac tcccgccacc cggcggtgcg ggtggtctgc
      721 gtgtccgacg gcgcggcggt ggccgagaag tccgacggca agggcaaggg cctgaccccg
      781 ctcgcgttcg cgccggtgcc aggcgacggc cgactgctcc tgctgcacga gcgccgcggc
      841 cgcgaggagt tgctggtgtg ggacgtgctc accgacacgg agacggagct cgccctcgac
      901 ctgccgggcg aggtggtcgc cgacttctac ccggacggac ggagcctgct cgtctggcac
      961 acccacgccg cccgcacccg gctgcaccgc tacgacctgg agacggggga actgacggaa
     1021 ctacccgtgg cgccggggtg cgtcggttcg gccgaggtcc ggccggacgg caccgtcgag
     1081 tacacctgct cgtcggccgc cgagccctcg gccgtgcgag ccctgcaccc cgacggcacc
     1141 gaccgcgtgc tcgtcgcgcc tcccggcgag cgcgccccag ggtcggtgcc cgtcgaggac
     1201 ctgtgggtcg acgggcccgg cgggcgggtc cacgcgctcg tcgcccgtcc ggccggagtg
     1261 gagggaccgg cgcccaccgt ggtgaccctg cacgggggcc cgcacgcggc cgacgaggac
     1321 cggttcgatc ccgggcgggc cgcctgggtg gacgccgggt ttctcgtcgt cgaggtgaac
     1381 taccgcggct cgacgggcta cggctcgaca tggcgcgacg ccatcgaggg caggccgggc
     1441 ctgaccgagc tggaggacgt ggccgcggtg ctggaccact gtgtcgcggc gggactcgcg
     1501 gacgccgacc ggtgcatcgt cgagggctgg tcgtggggcg gctatctggc actgctggcc
     1561 gtcggtaccc agccggaacg atgggcggcc gggctcgcgg gcgttccggt ggccgactac
     1621 ctcaccgcct acgccgacga gatggagcag ctccgtgcgt tcgaccgggc cttgttcggc
     1681 gggtcgccgc aggagcgacc ggaggcgtac cgcgcctcgt cgccgctgac ctacgtgaac
     1741 gaggttcgga cgccggtgct cgtgctggcg ggcgagaacg acccgcggtg cccgatccag
     1801 caggtggaca actacctcga cgccctcgcc gcgcgaagcg gcggcggaac gggcaccggc
     1861 gccgcccgcc gcactgggct ttacgaggtg agccgcttcg atgccggcca cggttctctg
     1921 gtcgtggacg agacactgcg gcacgtcgcc acggagatcg gcttcgccct acgcgctgtg
     1981 gcgtgacgcc gctcgtgcgc ggatacgggt gccggagcac ccgtatccgc gcacgacgcc
     2041 cccccgtcat ggtccggtag ccgaccggaa acatcccgag gggacgctgt agacgatccc
     2101 cgaggaggtg gaccacgtgg cactgtggcg ggtacatgtc cagctggagg accggcccgg
     2161 ccggctcggt gagctggcca cggcggtcgg cgcggccgga tgcaacatca tttccctaca
     2221 tgtcgtcggc gagccggccg acgacggttc gatcaccgac gagctcctcg tcaaggtgcc
     2281 ggagtcggcc gatcccgcca ccctcgtcga cgcggtggag agcgccggca tcccgtgcac
     2341 cctgctggtg cgggccgacg ccaccgagct gtccgatccg gccaccaccg cactcgcgct
     2401 ggcccggatg gtcgcggctg atcccggcag tggaccgcgc gcagtggcca cgatgctccg
     2461 cgcgcgactg gtcgacccgg ccgccgagaa ccacggcggg cacgtccacg tcatccgcgt
     2521 cggagctcag cagctgcgcc tcggccgggc ctggccgttc accgccacgg agctgtcacg
     2581 ggcggccgca ctgctggagc tcgccacgca gctcgcgatg cgcgctccca tccgggagac
     2641 gtcaccggag cggatcctgc tgctgcgcga cggctccgag gtgcgcatgc gacctgccgc
     2701 ctcggcggat gcccccctcg tggccgcgct gcacgcccgc tgcgcccccg attcgcggcg
     2761 atcccgcttc ctcagcccca cgcgtcagct cgcccccggc gagctggagg ccctcctcgg
     2821 aggggcgtcc ggctccggtc aggccgtgct cgcagtgacc gccgacggag gcagcgccgt
     2881 cggcatcgcg aacctcgaca gcgtgtggac gggctccgcc cgggccgctg ttctggtgga
     2941 ggactcctgg cagggccgag ggctgggcac cgcgctactg cggaggattg cggacatggc
     3001 caccgaacag gggatggcgg agctgaccgg catcgcccgc ccggatgacc tgggggtcac
     3061 ccggctgctg cgccgggcgg gcatgcgccc gtcggccgag atcgtggacg gcgaggtgcg
     3121 gctgcgcgcc ccgctccccg ccccggtcgc ctgaccgccg aacggcccgg gagcgcgtgg
     3181 tcccgggtcg ctcggcgacg ctgtcagagc gagatacgtt cccctgtgtc gccggcgaac
     3241 aggtgcaggg cgccttcacg gggggcgagg tgcagcaccg cgcccttgct gggcggcgta
     3301 cgcccgtcga cacgcgcgat gaccgggcgg cgctcgccgc ccacctcggt gctgccgtag
     3361 acgtaggcgt cggcgccgag ctcctcgacg acgtccacct ccgtgagcag gccggcgccg
     3421 ctctcgacga tgtccatgtc ctcgggacgg acgccgaccg tgaccgaccg ggtggccccg
     3481 acggcgtcgg cggcctgccg cggcacgcgg atgtcggcgc cgccgaagtg cagcacgtcg
     3541 ccgtcgacgt ccaccgggag caggttcatc gcaggtgagc cgatgaaccc cgcgacgaac
     3601 acgttgcgcg gcgcgtcgta catctcgcgc ggcgtgccga cctgctgcag gatcccgtcc
     3661 ttgagcactg cgacgcggtc acccatcgtc atggcctcca cctggtcgtg ggtgacgtag
     3721 acggtggtgg tgccgagcct gcgctgcaac gacgcgatct gcgtgcgggt cgagacgcgc
     3781 agcttggcgt ccaggttgga caatggctcg tccatgcaga acacctgcgg ctggcgcacg
     3841 atcgcgcggc ccatggcgac gcgctggcgc tgaccaccgg acagcgcctt ggggcgccgc
     3901 tccaggtagt ccacgaggtc gaggatcttc gccgcctcca gcacccgctc ccggatcatg
     3961 accttggact gcccggcgat cttgagcgcg aagcccatgt tctcggcgac ggtcatgtgc
     4021 gggtagagcg cgtagttctg gaaaaccatt gcgatgtcgc ggtccttcgg cggcacgcgc
     4081 gtgacgtcac gccccccgat gtggatggag cccttgtcga tgtcctccag cccggcgagc
     4141 atccgaaggg ccgtggactt gccggaaccc gagggaccca ccaggacgag gaactccccg
     4201 tcggcgacct ccaggtcgag cgagtcgacc gcgggagtgg tggagccggg gtagatccga
     4261 ctcgcgctgt cataggtcac agcagccatg ctgtgctcct tccaccggca ggaacgtgcc
     4321 ggacgatccg agtgtgaggg agtagcgctc gaagactgcc atgccgcgtg caatccgcac
     4381 aggactacta ctcactgtga cggtgaaaca cgctgcgtgg ggttaccgga tcgttactgc
     4441 tcgctgttgc cccctcaccg cgagagcgct tgcatgtcct gatcacgcgg tgcgggtgat
     4501 ttgcaccgtg tccttcccga tgaagcggag atgtgatgcc tgctgtgcca tcgaggaggc
     4561 ggtcgcgcac gaccggtctc gtgagcggcg ctctcggact gtgtctcgtg ctcgccggat
     4621 gcggcgggag caacgtcgcg ggcggaggtg gcggccaggc cggca
//
LOCUS       DASRSC010000019        22722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1642737,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000019 DASRSC010000000
VERSION     DASRSC010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 22722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22722
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1642737"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..147)
                     /locus_tag="VFE39_00695"
     CDS             complement(<1..147)
                     /locus_tag="VFE39_00695"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6257145.1"
                     /translation="MSHSFGGSYSRLVPGFIQSIERAAAVLRLLGTTDRPLGLKEIAA
                     ALDLP"
     gene            325..1128
                     /locus_tag="VFE39_00700"
     CDS             325..1128
                     /locus_tag="VFE39_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HET6257146.1"
                     /translation="MTKNSYIGELAAEFLGTMILLLFGAGVVAQVVAANLGNHDSIGW
                     AWGIGVILGVYVSGRISGGHINPAVTIALAAFQGFPWRKVLPYVVAQTAGAFVAALIV
                     RFAYADWINKVDPGHTYKTQIIFSTLPGNGEPGISLTTAFFDQIIGTAILVFVVFALV
                     NARNNPPLANLAPVVIGLLVVGIGMAWGANAGYAINPARDFGPRLASYITGYSTAWTD
                     QNGTPYWWLPIVAPIIGGLIGGALFQYTINRYLPADETEPTPVGRDIPR"
     gene            1146..2663
                     /gene="glpK"
                     /locus_tag="VFE39_00705"
     CDS             1146..2663
                     /gene="glpK"
                     /locus_tag="VFE39_00705"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET6257147.1"
                     /translation="MAEFVGAIDQGTTSTRFMIFDHSGNEVGRHQLEHQQILPQAGWV
                     EHNPVEIWDRTSSVLQTALVKTGLSSSDLVALGLTNQRETTVVWDKRTGRPLYNAIVW
                     QDTRTDRIASALEREGKGDVIRQKAGLPPATYFSGGKIQWILDNVDGAREAAESGNAI
                     FGNTDTWLLWNLTGGKDGGVHVTDPTNASRTMLMNLETLEWDDELLGFFGIPRSMLPE
                     IKPSSCPECYGTTLLRGPLSGEVVLTGDLGDQQAATVGQVCFSPGEAKNTYGTGNFML
                     LNTGTELVRSKAGLLTTVCYQFGDERPVYALEGSIAVTGSAVQWLRDQLGIISGASES
                     ESLARQVEDNGGVYFVPAFSGLFAPYWRSDARGAIVGLSRYNTNAHLARATLEAICYQ
                     SRDVAEAMEKDSGVHLEVLKVDGGVTANELCMQIQADVLGVDVSRPVVAETTALGAAY
                     AAGLAVGFWKNTDELRANWNEGKRWQPQWSDEQRSTGYERWKKAVTRTLDWVDVD"
     gene            2814..4472
                     /locus_tag="VFE39_00710"
     CDS             2814..4472
                     /locus_tag="VFE39_00710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET6257148.1"
                     /translation="MSSTELDILVVGGGVVGSGVALDAATRGLTVGLVEARDFASGTS
                     SRSSKLIHGGLRYLEMLDFRLVAEALQERGLLIEKLAPHLVRPVPFIYPLKHHVWERF
                     YAGAGVALYDTLGLLSGRARGVPHHRHLTRRGARKIMPSLRKDALVGALQYYDAQVDD
                     ARHTMFLARTAAAYGAHVASRARVVGLLREGERVTGAEVHDLESGRTIKVRARQVVNA
                     TGVWTDETQSLAGERGQFHVRASKGIHLVVPHDRIRGDSGLILRTEKSVLFVIPWGRH
                     WIIGTTDTDWTLGLAHPAASAADIDYLLDHVNEVLEQPLTHADVEGVYAGLRPLLSGE
                     SESTSMLSREHAVAHAVPGLVVVAGGKYTTYRVMAKDAVDAAVHGLYAKVPPSVTEDV
                     PLLGAEGFAALKNSSHVLAERYGLHEARIEHLLGRYGSLIHEVLDLVAQDPSLGEPLT
                     GAPDHLRAEIVYAASHEGARHLDDVLARRTRISIETFDRGLGSVEEVARLVAPVLGWS
                     DDQINREVEHYRKRVEAERESQKMPDDETADAARMGAPEVVPLR"
     gene            complement(4488..5375)
                     /locus_tag="VFE39_00715"
     CDS             complement(4488..5375)
                     /locus_tag="VFE39_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET6257149.1"
                     /translation="MSTAAADAARTAGRRFVVTGASSGLGLATARELARNGARVVLAV
                     RDVTAGERIAADLGGSTKVRALDLADLSSVRRFAESWDGDLDVLINNAGVGNLPKRRV
                     TADGFELQIGTNHLGHFALTNLLLPRITDRVVTVASGAHRFGGLDLADLNWESRPYRL
                     FASYGQSKLANLLFTLELQRRLVASGSPVRALAAHPGLAASNFGRHLGPVLGVVTKAV
                     GTVVQQSAEAGAAPTLFAATRDLPGASYVGPDGFGEVRGQPTLVGRSTQAADPDLARR
                     LWTLSEELTGVTFPLRAST"
     gene            complement(5413..5895)
                     /locus_tag="VFE39_00720"
     CDS             complement(5413..5895)
                     /locus_tag="VFE39_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257150.1"
                     /translation="MGLADRRRGWWGVPVLCASRMGLLSTRYEISRDGTTLSTWKPSP
                     FFGGGFFVLDGCRYDVARGGWSWRRYRLLDEAGELVALADGVGRSEWTLETGGVTHTF
                     ERPSLFRSEQVLISEGSTVGTVRRTSAWRGEAEADLPDLPLPVQVFVLVSLLGVWDED
                     "
     gene            5948..6949
                     /locus_tag="VFE39_00725"
     CDS             5948..6949
                     /locus_tag="VFE39_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HET6257151.1"
                     /translation="MSDLIGIDDITAAAERIAGHVLRTPTVPSPGLTAHLGAPVTLKL
                     ELLQRSGSFKPRGAFHKLLGLTPAERAVGVVAVSGGNHGLALADVAGSLGIAATVVMP
                     ESAPQRSIAACRASGATVRLTPDMPGAFSLLDELVAGGLTLVHPFDDPIVAAAQGTVG
                     AEFAADAPDVTDVLVSIGGGGLISGVATAFHAHVPGVRVWGVETEGADAMTRALAAGT
                     PAAITPTSIASTLSAPHVSQLTLDHVRVLVEDVIVVSDADAVRGTLTLAEEAKVWAEP
                     AAGCLVPAAVQVLDRVGPDARLGLVICGGNVTTADVGGWVQRFSATPLPRRPAPAVR"
     gene            6990..8048
                     /locus_tag="VFE39_00730"
     CDS             6990..8048
                     /locus_tag="VFE39_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET6257152.1"
                     /translation="MDVDVVVIGAGQAGLSAAYGLRRAGLEPDAGFVVLDGEHGPGGA
                     WRHRWPTLRVETTHRVSDLPGLPFAPADRSRPAAEVVPAYFDAYERAFDLRVHRPVRV
                     AAVRDAAPDLLVETDAGTWTTRALINATGTWTRPFRPHYPGQEIFRGRQLHVADYRGP
                     AEFTGLRVLVVGGGTSAVQLLMEIATVAAATTWVTRRSPQFHDGPFGDEVGRAAVARV
                     EERVRAGLPPQSVVGVTGLMLTPQIRAAQESGVLHRLPMFDRITPDGVAWADASTAAA
                     DVILWATGWRAALGHLAPLRLRGPGGGIAVDGTRVRSDPRIHLVGYGPSASTIGANRA
                     GRAAAHEVVSLLQEPLTA"
     gene            complement(8038..9189)
                     /locus_tag="VFE39_00735"
     CDS             complement(8038..9189)
                     /locus_tag="VFE39_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HET6257153.1"
                     /translation="MLPLLPVLLAVLAAVVACAGRAPPAVAAPPMAAAAPVADQLPGM
                     PPVLDTQNIYSAAGATMLSDAAKAARPLVYVPHTKSGDVWVIDPATFTVVAKHRLGGE
                     LQHVVPSYDMTTLYATDDIRHKVTPFDPRTGEPGASIPVIDPYNMYFTPDGRSAISVA
                     EARRQLVFYDPHTWQLQGTVWTPDCAGIDHADFTADGRTAVFSCEWAGRVAVVDVASR
                     RLLRMIDMPVRNTKMGPQDIRLAPDGSVFYIADSQQNGLWVLDGAATTVLRHIPTGHG
                     AHGLYLSRDAKRLFVTNRHEGSISVLDAYTGAALAKWTVPGGSPDMGNITADGSQLWL
                     SGRYDRAVYVLDTADGRLIHKFDVGRGPHGLCVWPQPGRYSLGHTGITR"
     gene            complement(9385..10119)
                     /locus_tag="VFE39_00740"
     CDS             complement(9385..10119)
                     /locus_tag="VFE39_00740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6257154.1"
                     /translation="MRILITGGTSGLGLAMARALADDGHRIVITGRAADRAAEVAATL
                     SGEVHGIGMDVRDSRSVRSGRDRTIDLLGGLDVLVNNAGIGMRTVNPGFLTEPQPFWQ
                     VPESGFRDVIDTNLTGYFLVASAVVPDLLASPGGQIINVTMNHATMTRSGFVPYGPSR
                     AGAESLSRIMAADLAGTGVRVNLLLPGGATATGMIPDDLADTSGLLPPEIMAVPVRWL
                     CSPAAAEIHDQRIVATEFHDGLATHG"
     gene            10483..11241
                     /locus_tag="VFE39_00745"
     CDS             10483..11241
                     /locus_tag="VFE39_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6257155.1"
                     /translation="MHVLYAGSLVNLMERDIGPKFAAASGYSYQGEGGGSAGLANEIK
                     GKVKQADVFISAGPSVNSSLEGAANGNQVSWYVTFASSPLVIGYNPHSRFAADLTSKP
                     WQQVLAEPGFRLGRTDPTTDPKGALAVQALNQEGLTSLAADQSEVFPETELVGRLQSG
                     QLDAGFFYSAEAIPDKIPTIRIPPLSLGAAYTVTVLNSAPDRAGAIAFVNYLLGHDGS
                     STLTADGFQLTSPPAVSGDAGAVPAGLHPLIPAP"
     gene            complement(11252..13192)
                     /locus_tag="VFE39_00750"
     CDS             complement(11252..13192)
                     /locus_tag="VFE39_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6257156.1"
                     /translation="MAAACSADSTGDDGAPMGVTRERVAAGMFGTPLPWLAAVLVAYL
                     LVPIGAFLVRLARASSDQLTVPGIGAALVVSVVTASIATVVISAAGIPLGYLLSRAHG
                     RPAELLGVAVQLPLALPPLVSGLLLVYVIGPYTALGQLFDGQLTDDLTGVVLAQIFVA
                     APFLIVAARSAFAALDPELADVAATLGHSRWSRFARVALPGAWAGIRAGMLLAWLRAF
                     GEFGATVVLAYHPYSLPVFTYVEFGSSGVDTTMIPVAAALVAAALVLALSSIAGRRHR
                     RHPARAIPPPPREPTAQAPVPLNFDLDARSGGFHLRLGHAARGRHLAILGPSGAGKSL
                     TLKLLAGLAPLREGQVRLGDRDLTTLAPESRGVGYVPQDSCLLPHLPVWRQITFGVDS
                     DPAVAAHWLGRLRLDGLEDRLPHQLSGGQRRRVALARALARQPRLLLLDEPFTGLDTP
                     VRDELRRELRRVQRDTAVTTVLVTHDPEEAALLADEVLLITDGGLLQGGSQHEVFARP
                     AGPAAARLLGIRNLHEGRMRDAETLDSCGTAITAGPVGLPAGADVVWCIRPEHVELHP
                     APPRTCGEHQATIVDTVHLGAVAELLVVLDGGCELTVVAADDAVRASGTRCRVSLPPE
                     RLIVWPVPTLSESQDRAAVARS"
     gene            complement(13306..14754)
                     /gene="gndA"
                     /locus_tag="VFE39_00755"
     CDS             complement(13306..14754)
                     /gene="gndA"
                     /locus_tag="VFE39_00755"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET6257157.1"
                     /translation="MTVADAKSRIGVTGLAVMGANLARNIARRGTPVAVHNRTTARTR
                     EFMEAYGDEGAFTATESVEDFVAALERPRRIIVMVKAGAPVDGVIEELTPLLDEGDII
                     IDAGNSHFPDTKRRTEACAERGLRFMGVGVSGGEEGALLGPSIMPGGDPAAYAEVEEI
                     FTGIAAVVDGTPCCVHVGPDGAGHYVKMVHNGIEYADIQLIAEAYDLLTHVAGLDAPA
                     IAKIFEEWNSGDLESFLIEITAKVLAKTDERTGGPLVDVILDQAEQKGTGRWTAIDAL
                     DLGVPLTGITEAVFARTLSSLSTERKAASGTLAGPVPGAGEDRTDLVDDIRQALYASK
                     VVAYAQGFAQMRAASKANDWDLDLGAMATIWRGGCIIRAQFLNRIRDAYAEHGDIENL
                     LMVPYFTEAVANAQDAWRRVVVTATQQGVAIPAFASSLSYYDAYRRERGPANLIQGLR
                     DFFGAHTYRRTDTEGSFHTRWGQDGTEVRTDA"
     gene            complement(14904..15776)
                     /locus_tag="VFE39_00760"
     CDS             complement(14904..15776)
                     /locus_tag="VFE39_00760"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET6257158.1"
                     /translation="MEIGLHIADFTYPAGPAGLAEDLTRIATAAEEAGFARVSVMDHV
                     WQISHIGPPENDMLEAYTTLGYLAARTSRVQLLAWVTAVTYREPGMLAKLVSTVDVLS
                     GGRAWLGIGAAWNGDEARGLGLPFPPTAERFERLEEALQICLQMWGPDDGPYEGKHYR
                     LERTLNVPQPLRRPHPPILIGGGGERKTLRLVAQYAQACNLFANADLEHKLDVLRGHC
                     EAVGRPYDDIEKTVMFPLDPGEKGENIDAILEQLQRFAGLGISEVHGRVDRAWEIEPL
                     KLIGREIVPAAAKL"
     gene            complement(15874..16668)
                     /locus_tag="VFE39_00765"
     CDS             complement(15874..16668)
                     /locus_tag="VFE39_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HET6257159.1"
                     /translation="MEPRDPAVPGLLRRGRIEITGRLVDASNATLFGTVTTNGLEAQC
                     VYKPVRGERPLWDFPDGTLAGREVATYLVSEAAGFDVVPPTVLRDGPFGPGMVQIWVD
                     TDDERELVDVRPPSDVPPGWLGVLRARGDRGKPAVLVHADTPELQTMAAFDVVTNNAD
                     RKGGHVLAGVDGNVYGVDHGLCLHTEEKLRTVLWGWVGRPLPEHVVEALQRLRSELED
                     GLCDALSEHITRREMRTLSARVDGLLDDPCYPEPSGYGPAIPWPAF"
     gene            complement(16692..17258)
                     /locus_tag="VFE39_00770"
     CDS             complement(16692..17258)
                     /locus_tag="VFE39_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HET6257160.1"
                     /translation="MSRVIHVFRQPERFVAGTVGEPGDRSFFLQAIEDARTISVLLEK
                     QQVSVLADRISALLHEVARRFGDEVPDEAQGSDLDPLAVPLEEEFRVGTMGLGWDADS
                     RSIVVELLAVTDEEVDESVVLDDTEEGPDALRVFLSPVQARAFADRAERVVSAGRPPC
                     PLCAEPLDPEGHVCVRLNGYHERSPVGD"
     gene            complement(17309..18013)
                     /locus_tag="VFE39_00775"
     CDS             complement(17309..18013)
                     /locus_tag="VFE39_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6257161.1"
                     /translation="MTTLILLRHGRSAANAAGVLAGRSPGVELDEAGLAQAQKVVERL
                     AGVPIAEIVCSPMVRCEQTVAPLAKDRGLTPVTEPELAEVDYGSWTGAALKTLFKEPL
                     WKIVQAHPSAAVFPGGEGLAQMQARAVAAVRRHGARIAADHGPTAVWLACSHGDVIKS
                     VLADALATHLDNFQRIVVDPSSISVVHYTDTRPFVARVNDLGGDVAGLIPPKPKRRRK
                     AAPRSSDAVVGGTTGS"
     gene            complement(18042..18863)
                     /locus_tag="VFE39_00780"
     CDS             complement(18042..18863)
                     /locus_tag="VFE39_00780"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET6257162.1"
                     /translation="MTWLEVLVLGVVQGLTEFLPISSSAHLRIVSEVFFGRDAGAAFT
                     AVTQLGTEAAVIVFFARDIGRLTATWFRGIRNAAVRATPDYRIAWLVIIGTIPIGLLG
                     VVFEEQIQTAARNLWLIATTLVLFGLLLGLAERVGRQRLELEQMKPVDGILLGFAQAM
                     ALIPGVSRSGGTITAGLFLGFTRPAAVRYSFLLAIPAVVASGLFQIPDVFSGDGPSPL
                     QMIVATVIAFAIGYGSIAWLLRYVERHSVYLFVWYRVALGALLFVALGAGWIAAT"
     gene            18914..19858
                     /locus_tag="VFE39_00785"
     CDS             18914..19858
                     /locus_tag="VFE39_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6257163.1"
                     /translation="MRRRRVGSSGLEVSQIGLGTMTWGADTDVDGATDQLTAFVDAGG
                     TLVETADGYGDGAAQETLGAVLGAAVPRDHLVIAGRAMLPGGALGDGAARGALLAGLD
                     ATLKRIGTDHLDLWQLPGFDADVPLEETLSAVQVAVMAGKARYAGLVAPKGWQLATVA
                     ERGRAMGGITVPVSAQVEYSLLSRDVEDDLLPAARHHGVGLLAWAPLGRGVLTGKYAD
                     GTPADSRAASPTLAPYVEARRNERSARIVQAVLTAADGLGTSPLSVALAWVRDRPGVS
                     AAIVGARDAAQLTASMAADSVVLPAEIRSALDDVSGPG"
     gene            19981..20220
                     /locus_tag="VFE39_00790"
     CDS             19981..20220
                     /locus_tag="VFE39_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5703 family protein"
                     /protein_id="HET6257164.1"
                     /translation="MDRSGPTGTVEGDSIDGEWEYRPVQLPGDVSRMTAAVRLAIQAE
                     FGGWELSRVRLYPGGVRKVWLRRRRSSRMLPELSV"
     gene            complement(20241..21014)
                     /locus_tag="VFE39_00795"
     CDS             complement(20241..21014)
                     /locus_tag="VFE39_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6257165.1"
                     /translation="MDLTGKAALVTGGASGIGAAAVRRLAAAGAVVAVADRDEAGARA
                     LAEEVGGLALPGDASDPDIMTMAVDVAEDAFGRLNIVLLNAGVTAGQSGVENLDLAAY
                     RRIVGVNVDHVVFGLTAAVPALRRAGGGTIVATASLAGLVAMPGDALYTMTKHAVVGY
                     VRAAAPTLVADGIRVNAVCPGFADTPLIANAKSRFGDFPLLAADDVAAAIETILERGE
                     PGECWFVQPGREPAPYGFRGVPGPKDGTPPPTGVLWEHH"
     gene            complement(21014..22318)
                     /locus_tag="VFE39_00800"
     CDS             complement(21014..22318)
                     /locus_tag="VFE39_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET6257166.1"
                     /translation="MAAEDEVVELASELIRIDTTNTGDPDTVVGEREAAEYVAAKLSD
                     VGYEVEVVESGAPRRDNVICRLKGADPDRGALLVHGHLDVVPADPTEWSVHPFSGAVQ
                     DGYVWGRGAVDMKDMVAMTIAVARRFKRDGVVPPRDIVFAFVADEEAGGKDGAQWLAA
                     NRPDLFEGCTEAVGEVGGFSLTLAEDRRVYLIEAAEKGLSWMRLRARGKPGHGSFLHD
                     DNAVTKVAEAVARLGNHTFPLVLTDTVRAFLEQMAELTGLQFPEDDLEGSLAKLGPLS
                     RIVGATIRDTANPTMLSAGYKANVIPSSAEAVLDCRVLPGREDAFLREVDELLGPDVG
                     REWITHLPAIETPFGGPLTDAMAAALRTEDPGAATVPYMLSGGTDAKSFADLGMNCYG
                     FAPLRLPPDLDFASLFHGIDERVPIDALKFGCRVLDTFLRSC"
     gene            22437..22521
                     /locus_tag="VFE39_00805"
     tRNA            22437..22521
                     /locus_tag="VFE39_00805"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22470..22472,aa:Leu,seq:gag)
BASE COUNT         3349 a         8105 c         8047 g         3221 t
ORIGIN      
        1 cggcaggtcg agggccgctg cgatctcctt cagcccgagc ggccggtccg tggtcccgag
       61 cagccgcagc accgctgctg ctcgctcgat ggactggatg aaccccggca cgagccgact
      121 gtacgacccg ccgaacgagt gagacacgca gcgctacgcg ccttcacgca cagcgagacc
      181 cccgcccccg gaggggaagc atgtgatgtt cgtccgatta gtagttcgac aatgccgaac
      241 gatgaccgtt gaccgcaggt cacgggcgtt tctagcgtgc gaggaccgcc cgtccgagcg
      301 gtatcccatt cctggagggc ttcgatgacg aagaactcgt acatcgggga gctggcggcc
      361 gagtttctcg gcaccatgat cctcttgtta ttcggcgccg gtgtggtggc gcaggtcgtg
      421 gccgccaacc tggggaacca cgactccatc ggctgggcct ggggcatcgg cgtgatactc
      481 ggcgtctacg tgtcgggccg gatcagcggc ggccacatca acccggccgt gacgatcgcg
      541 ctggctgcgt tccagggctt cccgtggcgg aaggtcttgc cctatgtggt cgcccagaca
      601 gcaggggcgt tcgtcgcggc cctcatcgtc cggttcgcct acgccgactg gatcaacaag
      661 gtcgaccccg gccacacgta caagacgcag atcatcttct ccacactccc gggtaacggc
      721 gaacccggga tcagcctcac gaccgcgttc ttcgaccaga tcatcggcac cgcgatcctg
      781 gtcttcgtgg tcttcgcgct cgtcaacgca cggaacaacc cgccactggc caacctggca
      841 ccggtggtca tcggtttgct cgtggtgggg atcggcatgg cctggggggc gaacgcgggc
      901 tacgcgatca accctgcccg tgacttcggt ccgcggctcg cgtcgtacat caccgggtac
      961 tccacggcct ggacagacca gaacggcacg ccctattggt ggctacccat tgtggcaccg
     1021 atcatcggtg ggctcatcgg cggcgcgctg ttccagtaca ccatcaaccg ctatctcccc
     1081 gccgacgaaa ccgagcccac tcccgtgggc cgcgacatcc cccgataacc gagcggagac
     1141 aactcatggc cgagttcgtc ggtgccatag accagggcac caccagcacc cgattcatga
     1201 tcttcgatca ctcgggtaac gaggtgggtc gccaccagct cgagcaccag cagatcctgc
     1261 cgcaggccgg ctgggtggaa cacaaccccg tcgagatctg ggaccgcacg tcatcggtgc
     1321 tgcagaccgc gctcgtcaag accgggctct cgtcgtcgga cctcgtggcg ttgggcctca
     1381 ccaaccagcg cgagaccacc gtcgtgtggg acaagcgcac cgggcgcccg ctctacaacg
     1441 cgatcgtctg gcaggacacc cgcaccgacc gcatcgcctc cgccctcgaa cgcgagggta
     1501 agggcgacgt catccggcag aaggccggtc tcccgcccgc cacgtacttc tccggcggaa
     1561 agatccagtg gatcctggat aacgtcgacg gcgcccggga ggccgcggag agcggcaacg
     1621 cgatcttcgg caacaccgac acctggctgc tgtggaacct cacgggcggc aaggacggcg
     1681 gcgtacacgt caccgacccg acgaatgcca gccgcacgat gctcatgaac ctcgagacgc
     1741 tcgaatggga cgacgagctg ctcggcttct tcggcatccc gcggtcgatg ctgcccgaga
     1801 tcaagccgag ctcgtgcccg gagtgctatg gcaccacgct gctgaggggg ccgctgagcg
     1861 gcgaggtcgt gctcaccggc gacctcggtg accagcaggc cgccaccgtc ggccaggtgt
     1921 gcttctcgcc cggcgaggcg aagaacacct acggcaccgg caacttcatg ctgctcaaca
     1981 cgggcacgga actcgtgcgg tcgaaggcgg ggctgctgac caccgtctgc taccagttcg
     2041 gcgacgagcg tccggtctac gcattggagg gctcgatcgc cgtcacgggg tcggccgtgc
     2101 aatggctgcg tgaccagctg gggatcatct ccggcgcctc ggagagcgag tcgctggccc
     2161 gccaggtgga ggacaacggc ggcgtgtact tcgtcccggc attctccggg ctgttcgccc
     2221 cgtactggcg ttccgacgcg cgcggcgcga tcgtcgggct ctcccggtac aacacgaacg
     2281 cccacctcgc gcgggcgacg ctggaggcga tctgctacca gagccgtgac gtggccgagg
     2341 cgatggagaa ggactcgggc gtgcacctgg aggtgctcaa ggtcgacggc ggcgtcactg
     2401 ccaacgagct gtgcatgcag atccaggccg acgtcctcgg cgtggacgtg agccggcccg
     2461 tcgtcgcgga gaccacggcg ctcggcgcgg cgtacgcggc ggggctcgcc gtggggttct
     2521 ggaagaacac cgacgagctg cgggccaact ggaacgaagg caagcgctgg cagccccaat
     2581 ggagcgacga gcagcgctcg accgggtacg agcgctggaa gaaggcggta accaggacct
     2641 tggactgggt agacgtcgac tgagtaggca cctgtacagc gggacgacat cgccgacgtc
     2701 gaaacctcag cgatgtcgcc ccgctgaacc gcccggcacg acaccgatga gaggaattac
     2761 ccatgagatc ggtcgccctc tctccgcagg cccgcgaggc cgccctggat gccatgagct
     2821 cgaccgaact cgacatcctg gtcgtcgggg gcggtgtcgt gggcagcggg gttgccctcg
     2881 acgccgcgac ccgcggcctc acggtcgggc tcgtcgaggc gcgggacttc gcctcgggta
     2941 cgtcgagcag gtcgagcaag ctcatccacg gcggcctgcg ttacctggag atgctggact
     3001 tccggctcgt cgccgaggcc ctgcaggaac gcgggttgct gatcgagaag ctcgcgccgc
     3061 acctcgtgcg gcccgtgccc ttcatctacc cgctcaagca ccacgtgtgg gagcggttct
     3121 acgccggtgc cggcgtcgcg ctctacgaca cgctcgggct gctgtccggg cgggcgcgcg
     3181 gcgtgccgca ccaccgccac ctgacgcggc gcggcgctcg caagatcatg ccgtccctgc
     3241 gcaaggacgc gctggtgggc gccctgcagt actacgacgc gcaggtcgac gacgcccggc
     3301 acacgatgtt cctcgcgcgc accgccgccg cctacggcgc gcacgtcgct tccagggctc
     3361 gcgtggtcgg gctgctccgc gagggcgagc gggttaccgg agcggaggtg cacgacctgg
     3421 agtccggccg cacgatcaag gtgcgcgccc ggcaggtcgt caacgccacc ggggtgtgga
     3481 cggacgagac gcagagcctg gcgggcgagc gcggccagtt ccacgtgcgg gcgagcaagg
     3541 gcatccacct cgtcgtgccg cacgaccgga tccgtggcga ctccgggctg atcctgcgca
     3601 ccgagaagtc ggtgctgttc gtcatcccgt ggggcaggca ctggatcatc ggcaccaccg
     3661 acaccgactg gaccctcggg ctggcccatc cggcggcgag cgcagccgac atcgactacc
     3721 tgctcgacca cgtcaacgag gtgctggagc agccgctcac ccacgccgac gttgagggcg
     3781 tctacgccgg gttgcggccg cttctctcag gcgagtcgga gtcgacgtcg atgctgtcgc
     3841 gcgagcatgc cgtggcgcac gcggtgccgg gactcgtggt ggtggcaggc ggcaagtaca
     3901 ccacctaccg cgtgatggcc aaggacgccg tcgacgcagc ggtgcacggg ctgtacgcca
     3961 aggtgccgcc gtccgtgacg gaggacgttc ccctgctggg ggccgagggc ttcgccgcgc
     4021 tgaagaactc ctcgcatgtc ctggccgagc ggtacgggct gcacgaggcc cgtatcgagc
     4081 acctgctggg gcggtacggg tcgctgatcc acgaggtgct cgacctcgtc gcgcaggacc
     4141 cgtcgctggg cgagccgctg accggggcgc ccgaccacct gcgggccgag atcgtctacg
     4201 cggcctcgca cgagggcgcc cggcacctcg acgacgtgct cgcgcgccgc acccggatct
     4261 cgatcgagac cttcgaccgg gggctcgggt ccgtcgagga ggtcgcgcgg ctcgtcgcac
     4321 ccgtcctggg ctggagcgac gaccagatca accgcgaggt ggagcactac cgcaagcgcg
     4381 tcgaggcgga gcgggagagc cagaagatgc ccgacgacga gacggccgac gccgcgcgga
     4441 tgggcgcacc ggaggtcgtt ccactgcgct gaggcgtacg ttcgcgttca cgtcgaggca
     4501 cgcagcggaa acgtgacgcc ggtgagctcc tcggagagcg tccacagtcg gcgggcgagg
     4561 tcggggtctg cggcctgcgt ggacctcccg acgagggtgg gctggccgcg cacctcgccg
     4621 aagccgtcgg ggccgacata gctggcaccg ggcaggtcac gggtggcggc gaacagcgtg
     4681 ggcgccgcgc cggcctccgc gctctgctgc acgaccgtgc cgacggcctt cgtgacaacg
     4741 cccaggacgg ggcccagatg gcggccgaag ttcgacgcgg ccaagccggg gtgggcggcg
     4801 agcgcgcgaa ccggcgagcc ggaggcgacc aggcgtcgct gcagttccag cgtgaacagg
     4861 aggttggcca gcttcgactg cccgtaggag gcgaagagcc ggtacggccg ggactcccag
     4921 ttgaggtcgg cgaggtcgag gccgccgaac ctgtgcgcgc cggacgcgac cgtgaccacg
     4981 cggtcggtga tgcgcggcag cagcaggttg gtcagcgcga aatgaccgag gtggttggtg
     5041 ccgatctgca attcgaaccc gtcggccgtg acgcgccgct tcggcaggtt ccccaccccc
     5101 gcgttgttga tcaggacgtc gaggtcgccg tcccacgact cggcgaaccg gcgcacagag
     5161 ctgagatcgg ccaggtccag ggcacggacc ttggtggagc cgccgaggtc cgcggcaatg
     5221 cgttcgcccg cggtcacgtc acgaacggcc agcacgaccc gggcgccgtt ccgggcgagc
     5281 tcgcgcgcgg tggcgaggcc gagcccactg ctcgcccccg tgacgacgaa acgacggccg
     5341 gcggtccgag cagcatccgc tgcagcagta ctcataacca tcaaaagtag gcaccgggcg
     5401 ggtgaggtgt cactaatcct cgtcccagac gcccagcagg ctcaccagca cgaacacctg
     5461 gaccggcagc ggcaggtcgg ggaggtcggc ctccgcctcg ccccgccacg cgctggtgcg
     5521 ccgcaccgtc cccacggtcg agccctcgct gatcagcacc tgctcgctgc ggaacagcga
     5581 cggtcgctcg aaggtatgcg tcaccccgcc ggtctcgagc gtccattccg accggcccac
     5641 cccgtcggcc agcgcgacga gctcgcccgc ctcgtcgagg agccggtagc ggcgccacga
     5701 ccacccgccg cgggccacgt cgtaacggca gccgtcgagc acgaagaaac cgccgccgaa
     5761 gaacggcgac ggcttccagg tggacagtgt ggtgccatcg cggctgatct cgtaacgggt
     5821 gctcagcagc cccatccgcg acgcgcacag cacgggcaca ccccaccacc cccgccgccg
     5881 atccgccagt cccatcgcct ggagggggag aagggccggg cgcccagcca gcgttagcgt
     5941 tggcagggtg agcgacctga tcggcatcga cgacatcacg gcggcagcgg agcggatcgc
     6001 ggggcacgtg ctacgcaccc ccactgtccc gagcccggga ctcaccgccc acctcggtgc
     6061 acccgtcacc ctcaagctgg agctgctgca gcgctccggc tcgttcaagc cgcgcggcgc
     6121 gttccacaag ctgctcgggc tcacccctgc cgagcgggcg gtcggggtgg tggcggtcag
     6181 cggcggtaac cacgggctcg cgctggccga cgtcgccggg tcgctgggca tcgcggccac
     6241 cgtcgtgatg ccggagtcgg cgccacagcg ctcgatcgcg gcctgccgcg cgtccggtgc
     6301 gaccgtgcgc ctcaccccgg acatgcccgg cgcgttctcg ttgctcgacg agctcgtggc
     6361 gggcgggctc acgctcgtgc acccgttcga cgacccgatc gtcgccgccg cccagggcac
     6421 cgtcggtgcg gagttcgccg ccgacgcgcc cgacgtcacc gacgtgctgg tcagcatcgg
     6481 cggcggcggc ctgatctcgg gtgtggccac ggcgttccac gcccacgtgc ccggcgtccg
     6541 ggtctggggc gtagagaccg agggggccga cgcgatgacg cgcgcgctcg ccgccggcac
     6601 ccccgccgcg atcacgccga cctcgatcgc ctccacgctc agcgcgccgc acgtttcaca
     6661 gctcacgctc gaccacgtcc gggtgctcgt cgaggacgtg atcgtcgtct ccgacgccga
     6721 cgccgtgcgc ggcaccctca ccctcgccga ggaggcgaag gtctgggccg agcccgcggc
     6781 aggttgcctg gttccggctg ccgtccaggt gctcgaccgg gtcggcccgg atgcccgcct
     6841 cggtctggtg atctgcggcg gcaacgtgac cacggccgac gtgggcggct gggtgcagcg
     6901 gttctcggcc acgcccctcc cccgccgccc tgctccagcg gtgcgctgac ccgcggaagc
     6961 ccggcgggcc gtccggcgtt ccaccgggcg tggatgtcga cgtcgtcgtg atcggggccg
     7021 ggcaggccgg gttgtcggcg gcgtacgggc tgcgccgcgc cggtctcgag ccggatgcgg
     7081 gattcgtggt gctggacggc gagcacggcc ccggcggggc gtggcggcac cgctggccga
     7141 cgttgcgggt cgagacgacc caccgcgtca gtgatctgcc cgggctgccg ttcgcccccg
     7201 ccgaccggtc gcggcccgca gccgaggtgg tgcccgccta cttcgacgcc tacgagcgcg
     7261 cgttcgacct acgggtgcac cgcccggtgc gcgtcgctgc cgtgcgcgac gccgcccccg
     7321 acctccttgt cgagaccgat gccggcacct ggaccacccg cgccctgatc aacgccaccg
     7381 ggacctggac gcgccccttc cgcccccact accccgggca ggagatcttc cgcggccgcc
     7441 agctgcacgt cgccgactac cgcgggcccg cggagttcac cgggctgcgg gtgctggtgg
     7501 tcggcggggg gacgtcggcc gtgcagctgc tcatggagat cgccacggtc gccgcggcca
     7561 cgacgtgggt gacgcggcgg agtccccagt tccacgacgg cccgttcggc gacgaggtgg
     7621 gacgcgccgc ggtggcccgc gtcgaggagc gggtgcgggc gggcctgccc ccgcagagcg
     7681 tcgtcggcgt gaccgggctg atgctcaccc cgcagatccg ggccgcgcag gagtcgggag
     7741 tgctgcaccg gctgcccatg ttcgaccgca tcaccccgga tggggtggcg tgggccgacg
     7801 cctctaccgc tgcggccgac gtgatcctgt gggccaccgg ctggcgcgcc gcgctgggac
     7861 acctcgcccc gttgcggctg cgcggaccgg gcggcggcat cgcggtggac ggcacccggg
     7921 tgcggtccga cccacggatc cacctggtgg gctacggccc gagcgcgagc acgatcgggg
     7981 ccaaccgggc cgggcgggcg gccgcgcacg aggtggtgtc cctcctgcaa gagccgctca
     8041 ccgcgtgatc ccggtgtggc ccagcgagta gcggccgggc tgcggccaca cgcacaaccc
     8101 gtgcggcccg cgcccgacgt cgaacttgtg aatgaggcgc ccgtcggcgg tgtccagcac
     8161 gtagaccgcg cggtcgtagc gccccgacag ccacagctgg ctgccgtcgg cggtgatgtt
     8221 gcccatgtcc gggctgccgc cggggaccgt ccacttggcg agcgcggcgc cggtgtaggc
     8281 gtccagcacg ctgatgctgc cctcgtgccg gttggtgacg aacagcctct tcgcgtcacg
     8341 ggagaggtag agcccgtgcg cgccgtgtcc ggtgggaatg tggcgcagca ccgtggtggc
     8401 ggcgccgtcg agcacccaga gcccgttctg ctggctgtcg gcgatgtaga acaccgagcc
     8461 gtccggggcg agcctgatgt cctgtgggcc catcttcgtg ttccgtaccg gcatgtcgat
     8521 catgcgcagc agccggcgcg atgccacgtc gaccaccgca accctgccgg cccactcgca
     8581 gctgaacacg gccgtgcgtc cgtcggcggt gaagtcggcg tggtcgatgc ctgcgcagtc
     8641 gggggtccac accgtgccct gcagctgcca cgtgtgcggg tcgtagaaca cgagctgcct
     8701 gcgcgcctcg gccaccgaga tcgcggaccg gccgtccggc gtgaagtaca tgttgtacgg
     8761 gtcgatcacc gggatgctcg cgcccggctc accggtcctc gggtcgaacg gcgtcacctt
     8821 gtgcctgatg tcgtctgtgg cgtagagcgt ggtcatgtcg taggacggca cgacgtgctg
     8881 gagctcgcca cccagccggt gcttcgcgac gacggtgaag gtcgccgggt cgatcaccca
     8941 gacgtcgccc gacttcgtgt gcgggacgta caccaggggc ctggctgcct tcgcggcgtc
     9001 ggagagcatc gtcgccccgg cagcggagta gatgttctga gtgtcgagga cgggcggcat
     9061 cccgggcagc tggtcggcca ccggcgcggc cgccgccatc ggcggcgccg cgacggcagg
     9121 aggcgcccgg ccggcgcagg ccaccacagc ggcgaggacg gccagcagga ccggcagcag
     9181 cggcagcacc ggggcatggc accccctcct gcaccaccac ccgccgccga tgctcgccat
     9241 tggccggaga ttaccgcccc tgacctggga attccatctc tcaccgacca cgtgggagag
     9301 ggcgttcccg ctgcgctcgc gtccatgtgc agcacctccc aggggtgccc gtgcaggccg
     9361 tccggccggc gccgcggcgg ctactcatcc gtgggtggcc agcccgtcgt ggaactcggt
     9421 ggcgacgatg cgctggtcgt gtatctcggc ggcagcggga gaacagagcc agcgcaccgg
     9481 cacggccatg atctcggggg gcagcaggcc cgaggtgtcg gcgaggtcgt cggggatcat
     9541 gccggttgcg gtggcacctc ccggcaggag caggttcacc cggacaccgg tgcctgccag
     9601 gtcggccgcc atgatgcggg acagcgactc ggcgcctgcg cgggaggggc cgtaggggac
     9661 gaacccggac cgcgtcatcg tcgcgtggtt catggtgacg ttgatgatct gtccaccggg
     9721 ggatgccagc aggtcgggca cgacagcgga ggcaaccagg aagtagccgg tcaagttggt
     9781 gtcgatgaca tcgcggaacc cggattccgg cacctgccag aacggctggg gctcggtgag
     9841 gaagcccggg ttcaccgtcc gcatgccgat gcccgcattg ttgaccagca cgtccaggcc
     9901 accgagcagg tcgatcgtac ggtcgcggcc ggaccggacc gaccggctgt cgcgcacgtc
     9961 catcccgatg ccgtgcacct caccggacag cgtcgcggcg acctccgcgg cgcggtcggc
    10021 cgcccggccg gtgatgacga tccggtgccc gtcgtcggcg agcgcccgcg ccatcgccag
    10081 gcccagaccg ctcgttcctc ccgtgatcag gattcgcatg gtgcccagtc tgcgcctgct
    10141 ccgcccacgt ccgcggtgac ggacatacgg atcgcagctc gccgcccacc gcatctgtcc
    10201 ggtaccggag gccgtgtccg aagtagcgcc tcggcctcgc gaccagttgt cccgcctgag
    10261 cagcgaccac ggctgtaact ccgtaatgtc tccgtaggta cggagaaagc aggctgctat
    10321 cgccgttatc gcggagtcat cgcggcgacg acgaactgga ggagctcgat gaggtcgtgg
    10381 cgtctcgtag gtgggctggc tgcggtggca ctcgcagtgg ccggctgttc gagcggcagc
    10441 ggtgcgagca cgagtgcctc ggcgacagcc ggaggacaac ccgtgcacgt gctctacgca
    10501 ggctcgctgg tgaacctgat ggagcgggac atcgggccga agttcgcggc cgcgagcggg
    10561 tacagctacc agggtgaggg cgggggttcc gcgggactgg ccaacgagat caagggcaag
    10621 gtcaagcaag cggacgtgtt catcagcgcc ggcccctcgg tcaactccag cctcgaggga
    10681 gcggccaacg ggaaccaggt gtcgtggtac gtgacgttcg cgtcgtcccc gctggtgatc
    10741 ggttacaacc cgcacagcag attcgccgcc gacctgacgt ccaagccgtg gcagcaggtg
    10801 ctggccgagc cgggcttccg gctggggcgc acggacccca ccaccgaccc gaagggcgca
    10861 ctggcggtgc aggcgctcaa ccaggagggc ctcacctcgc tggctgcgga ccagtcggag
    10921 gtgttccccg agacggagct ggtcggccgc ttgcagtccg gtcagctcga tgccgggttc
    10981 ttctactcgg cggaggccat tccggacaag atccccacga tccggatccc tccgctgtcg
    11041 ttgggcgccg cctatacggt cacggtgctg aactcggcgc ccgatcgggc cggagccatc
    11101 gccttcgtca actacctgct gggtcacgac ggctcctcga cgctcaccgc cgacgggttc
    11161 caactgacgt caccgcctgc ggtgtccggg gacgccgggg cagtgcccgc cggactccac
    11221 ccgctgatcc ccgccccctg acctacgggc atcagcttcg ggccacagcc gcccggtcct
    11281 ggctctccga gagcgtgggc accggccaga cgatgagccg ttccggaggt agggacaccc
    11341 ggcagcgcgt gccggacgcc cgcaccgcgt cgtcggccgc gacgacggtg agctcgcacc
    11401 caccatccag gacgaccagc agctcggcca ccgcccccag atggacggtg tcgacgatcg
    11461 tggcctgatg ctccccgcag gtgcggggcg gtgccgggtg gagctccacg tgttcgggcc
    11521 ggatgcacca cacgacgtcg gccccggccg gaaggccgac aggtcccgcc gtgatcgcgg
    11581 tgccgcagga gtcgagcgtc tcggcgtcgc gcatccggcc ctcgtgcagg ttgcggatac
    11641 ccagcaggcg cgcggccgcc gggccggccg ggcgggcgaa gacctcgtgt tggctcccgc
    11701 cctgcagcag acctccgtcg gtgatcagca ggacctcgtc ggcgagcaac gcggcctcct
    11761 cgggatcgtg ggtgaccagc accgtggtca ccgccgtgtc gcgttgcacg cgtcgcagtt
    11821 cccgtcgcag ctcgtcgcgg accggtgtgt ccagcccggt gaagggttcg tcgagaagca
    11881 gcagccgagg ttgccgggcc aaggccctgg ccagcgcgac gcggcggcgc tggccgccgg
    11941 acagctggtg cggcagccgg tcctcgaggc cgtcgaggcg caggcgcccg agccagtggg
    12001 cggccaccgc gggatcgctg tcgacgccga aggtgatctg ccgccacacc ggaaggtgcg
    12061 gaaggaggca ggaatcctgc ggcacgtagc cgacgccgcg ggactcgggg gcgagcgtgg
    12121 tcaggtcgcg gtcgccgagc cgcacctgtc cctcgcgcag cggagccagt ccggcgagca
    12181 gcttgagggt caacgacttc cccgctccgg acgggcccag gatggccagg tgccggccgc
    12241 gagcggcatg gcccagtcgg aggtggaatc cgcccgaccg ggcgtcgagg tcgaagttga
    12301 gcgggacggg ggcttgggca gtcggctccc ggggcggcgg cggtatcgcc cgggccgggt
    12361 ggcgacggtg acgacggccg gcaatgctgc tcaaggccag aaccagggcg gcggccacca
    12421 gagctgcggc caccgggatc atggtggtgt ccactccgga gctgccgaac tccacgtagg
    12481 tgaagaccgg cagcgagtag gggtggtagg ccagcaccac cgtggccccg aactccccga
    12541 acgcacgcag ccacgccaac aacatccccg cccggatccc ggcccacgcc ccgggcagcg
    12601 cgacccgcgc gaaccgtgac cagcgactgt gacccagcgt ggcggcgacg tcggccagct
    12661 cgggatccaa cgccgcgaac gccgaccgcg cggcgacgat caggaagggc gcagccacga
    12721 agatctgcgc cagcaccacg ccggtcaggt cgtcggtcag ctgaccgtcg aacagctggc
    12781 cgagcgcggt atagggcccg atcacgtaca ccagcagcag accgctgacc agcggcggaa
    12841 gggccagcgg cagttgcacc gccacaccga gcagctcggc cgggcggcca tgggcgcgtg
    12901 acagcaggta gcccagcggt atcccggccg cgctgatgac gaccgtggcg atgctggcgg
    12961 tcaccaccga cacgaccagt gcggcaccga tgcccggcac cgtcagttga tccgacgatg
    13021 cccgcgccag ccgtacgagg aaggccccga tcgggaccag caggtaggcc accaagactg
    13081 ccgcgagcca tggaagcggt gtcccgaaca tcccggccgc aacacgttcg cgcgtcacgc
    13141 ccatcggcgc cccgtcgtcc ccggtgctgt ccgcgctaca cgccgccgcc atcccttacc
    13201 acggtcggtt gcgagcacct gaccagagta tccgcgcgtg cggagaacag acggagacag
    13261 agacgcagat acggagagcc gtgccgaacg cgacaccagc cgcggtcagg cgtcggtgcg
    13321 cacctcggtt ccgtcctggc cccaccgcgt gtggaacgag ccctctgtgt cggtgcgccg
    13381 gtaggtgtgg gcgccgaaga agtcgcgcaa cccctggatg aggttcgcgg ggccgcgctc
    13441 gcgccggtag gcgtcgtagt aggacagcga cgacgcgaac gccgggatcg ccacgccctg
    13501 ctgcgtcgcg gtcacgacca cgcgccgcca ggcgtcctgc gcgttggcca cggcctcggt
    13561 gaagtagggc accatcagca ggttctcgat gtcgccgtgc tcggcgtacg cgtcgcggat
    13621 ccggttgagg aactgggcgc ggatgatgca gccgccgcgc cagatcgtgg ccatcgcgcc
    13681 caggtcgagg tcccagtcgt tcgccttcga ggcggcgcgc atctgcgcga agccctgcgc
    13741 gtacgccacg accttgctgg cgtagagcgc ctgccggatg tcgtcgacga ggtcggtgcg
    13801 gtcctccccg gcccccggga ccggcccggc cagcgtgccg gacgcggcct tgcgctccgt
    13861 gctcagcgac gacagcgtgc gggcgaacac cgcctcggtg atgccggtga gcggcacgcc
    13921 gagatcgagg gcgtcgatgg ccgtccagcg gccggtgccc ttctgctcgg cctggtcgag
    13981 gatgacgtcg acgagcgggc cgcccgtcct ctcgtcggtc ttcgcgagca ccttcgcggt
    14041 gatctcgatg aggaacgact ccaggtcgcc ggagttccac tcctcgaaga tcttcgcgat
    14101 cgccggcgcg tccaggccgg ccacgtgggt gagcaggtcg taggcctcgg cgatgagctg
    14161 gatgtcggcg tactcgatgc cgttgtgcac catcttcacg tagtgccctg caccgtccgg
    14221 cccgacgtgg acgcagcagg gtgtgccgtc gaccacggcg gcgatgcccg tgaagatctc
    14281 ctcaacctcc gcgtacgcgg cggggtcgcc cccgggcatg atgctcggcc cgagcagcgc
    14341 gccctcctcg ccgcccgaca ctccgacccc catgaaccgc agcccgcgct cggcgcaggc
    14401 ctcggtgcgt ctcttcgtgt cggggaagtg tgagttgccg gcgtcgatga tgatgtcgcc
    14461 ctcgtcgagc agcggcgtga gctcctcgat caccccgtcc accggggcac cggccttgac
    14521 catgacgatg atccgacgcg gccgctccag cgcggcgacg aagtcttcca cgctctccgt
    14581 ggccgtgaag gcaccctcgt cgccgtaggc ctccatgaac tcgcgtgtgc gcgccgtcgt
    14641 ccggttgtgc acggcgacgg gggtgccgcg ccgggcgatg ttgcgggcga ggttggcccc
    14701 catcaccgcc agcccggtga ccccgatgcg gctcttggcg tctgccacgg tcatcgctcg
    14761 ctctccctcg gtaggcccgc gcgacgttac ccgcggagcg ggtcgctcgg ctcgtcgccc
    14821 gcaccatcac ccacgctcaa cgtcctggca ttccccccgc cgtgagcaca tccggaagga
    14881 cgccccggcg aggcggccgg gcgtcagagc ttcgcggcag ccggcacgat ctcgcggccg
    14941 atgagcttca gcggctcgat ctcccacgcc cggtcgaccc gcccgtggac ctcgctgatg
    15001 ccgaggccgg cgaagcgctg gagctgctcg aggatcgcgt cgatgttctc gcccttctca
    15061 ccggggtcga gggggaacat gacggtcttc tcgatgtcgt cgtatgggcg gccgaccgcc
    15121 tcgcagtgcc cccgcagaac gtcgagcttg tgctcgaggt cggcgttggc gaacaggttg
    15181 caggcctgcg cgtactgggc gaccagccgc agtgtcttcc gctccccacc gccgccgatg
    15241 aggatgggcg ggtgcgggcg gcgcagcggc tgcgggacgt tcaatgtcct ctcgagccgg
    15301 tagtgcttcc cctcgtaggg gccgtcgtcg ggcccccaca tctgcaggca gatctgcagg
    15361 gcctcctcca ggcgctcgaa ccgctcggcc gtgggcggga acggcagccc gagcccgcgc
    15421 gcctcgtcgc cgttccaggc ggcgccgatc cccagccagg cacgcccgcc cgacagcacg
    15481 tcgacggtac tgaccagctt cgcgagcatc ccgggctcgc ggtaggtgac ggccgtgacc
    15541 caggcaagca gctggacccg cgacgtgcgc gccgcgaggt atcccagcgt ggtgtaggcc
    15601 tccagcatgt cgttctcggg cggcccgatg tgcgagatct gccagacgtg gtccatgacg
    15661 ctcacccgcg cgaaaccggc ttcctcggcc gcggttgcaa tccgggtgag gtcctcggcg
    15721 agcccggcgg ggcccgccgg ataggtgaag tccgcgatgt gcaggccgat ctccacgatg
    15781 tctctctccc tctaccgtgc gtcccagtac gtcagcgacg ctagtcccgt cgctacccgg
    15841 catgccttgc cggtgtctgg cggcacaagc ccgctagaag gccggccagg ggatggcagg
    15901 gccgtagccg gagggctcgg ggtagcacgg gtcgtccagc agcccgtcga cccgcgcaga
    15961 cagggtccgc atctcgcgcc gggtgatgtg ctcgctcagc gcgtcacaga gcccgtcctc
    16021 gagctcggag cgcagccttt gcagcgcctc cactacgtgc tcgggcaggg gcctgcccac
    16081 ccagccccac agcacggtgc ggagcttctc ctcggtgtgc aggcacaacc cgtggtcgac
    16141 gccgtagacg ttgccgtcga cgcccgcgag gacgtgcccg cctttgcggt cggcgttgtt
    16201 cgtcacgacg tcgaatgcgg ccatcgtctg cagctccggg gtgtcggcgt gcacgagcac
    16261 cgcgggcttg ccgcggtcgc cgcgcgctcg cagcaccccg agccaccccg gcggcacgtc
    16321 cgacggtggc cggacgtcca cgagctctcg ctcgtcgtcg gtgtccaccc agatctgcac
    16381 catccccggt ccgaacgggc cgtcgcgcag cacggtcggc ggcacgacgt cgaaacccgc
    16441 ggcctccgag accaggtacg tcgccacctc gcgcccggcg agcgtgccgt ccgggaagtc
    16501 ccatagcgga cgctcaccgc gcacgggctt gtagacgcat tgcgcctcga ggccgttggt
    16561 ggtcacggtg ccgaacaggg tggcgttcga ggcgtcgacg agtcgtccgg tgatctctat
    16621 ccggcctcgc cggagcagtc ccggcacagc cgggtcacga ggttccacgt gcgtcagcgt
    16681 cggtctcttc gtcagtcccc gaccggggaa cgctcgtggt agccgttcaa ccgcacacac
    16741 acgtggccct ccgggtcgag cggctcggcg cagagcgggc acggtggccg cccggcggag
    16801 acgacccgct ccgcccggtc ggcgaacgcg cgggcctgca ccggcgagag gaacacgcga
    16861 agggcgtcgg gcccctcctc ggtgtcgtcg aggacgacgg actcgtcgac ctcctcatcg
    16921 gtgacggcga gcagctcgac gacgatggag cgcgagtcgg cgtcccagcc caggcccatc
    16981 gtgccgaccc ggaactcctc ctccaacggc acggcgagcg ggtcgaggtc actgccttgg
    17041 gcctcgtcgg gaacctcgtc gccgaagcgg cgggccactt cgtggagcaa cgcgctgatt
    17101 cggtcggcga gcacggagac ctgctgtttc tccagcagga cactgatggt gcgagcgtcc
    17161 tcgatggcct gcaggaagaa cgagcggtcg ccgggttcac cgacggtccc ggcgacgaag
    17221 cgctcgggct ggcggaagac gtggatgacg cgtgacatgg cgctactcag aataggccac
    17281 ctcggcgccc acggcacctc ctcagcggtc agctgcccgt ggtcccgccc accacggcgt
    17341 cggagctgcg gggcgcggcc ttgcgcctgc gcttgggctt gggcgggatc agcccggcga
    17401 cgtcgccgcc gaggtcgttc acccgcgcga cgaacggccg ggtgtcggtg tagtgcacga
    17461 cgctgatgga gctcgggtcg acgacgatgc gctggaagtt gtccaggtga gtggccagcg
    17521 catcggccag cacggacttg atcacatcac cgtggctgca ggccagccag accgccgtcg
    17581 gcccgtgatc ggcggcgatg cgcgccccgt gcctgcgcac ggcggccacg gcgcgggcct
    17641 gcatctgcgc cagtccctca ccgccgggga acaccgcggc ggacgggtgc gcctgcacga
    17701 tcttccacag cggctccttg aacagcgtct tcagggcagc cccggtccag gagccgtagt
    17761 cgacctcggc gagctcaggc tccgtcaccg gcgtcagtcc gcggtccttc gccagcggcg
    17821 ccacggtctg ctcgcagcgc accatcggcg agcagacgat ctccgcgatg ggaacgcccg
    17881 cgagccgctc cacgaccttc tgcgcctgcg ccaaccccgc ctcgtccagc tcgacgccgg
    17941 ggctgcgccc ggcgaggaca ccggccgcgt tggctgcgga ccgcccgtgg cggagcagga
    18001 tgagggtcgt cacgatcacc gagcctacgt ggagcagccg ctcaggtcgc tgcgatccat
    18061 ccggcgccca gcgcgacgaa cagcagcgca cccagtgcca cccggtacca gacgaacagg
    18121 tacacgctgt gccgctcgac gtagcgcagc agccaggcga tcgagccata gccgatggcg
    18181 aacgcgatga ccgtcgcgac gatcatctgc agcgggctcg gcccgtcacc ggagaagacg
    18241 tcgggaatct ggaagagacc cgacgcgacg acggccggga tcgcgagcag gaacgagtag
    18301 cgcacggccg cggggcgggt gaagccgagg aagagtccgg cggtgatcgt gccgccggag
    18361 cgggagaccc cggggatcag tgccatcgcc tgcgcgaacc cgaggaggat gccgtccacc
    18421 ggcttcatct gctccagctc gaggcgctgg cggcccaccc gctcggccag cccgagcagc
    18481 agaccgaaca ggaccagcgt ggtcgcgatg agccagaggt tgcgggccgc ggtctggatc
    18541 tgctcctcga agaccacccc gagcaggccg atcgggatcg tgccgatgat cactagccac
    18601 gcgatccggt agtccggcgt ggcgcggacg gccgcgttgc ggatgccgcg gaaccaggtg
    18661 gcggtcaggc ggccgatgtc ccgggcgaag aacacgatca ccgcggcctc ggtgccgagc
    18721 tgggtgaccg ctgtgaacgc ggcacccgcg tcccgcccga agaacacctc cgacacgatc
    18781 cgcaggtgag ccgacgagga gatcggcaag aactccgtca ggccctgtac gacaccgaga
    18841 acgagcactt ccagccaagt cacggcgcgg accgtaccga ctgcgaccgg gcgctcacgc
    18901 ctaggctctt cctgtgcgac gtcggcgggt cgggagcagc gggctggagg tgtcgcagat
    18961 cggcctcggc accatgacgt ggggcgcgga caccgacgtc gacggcgcca ccgaccagct
    19021 caccgcgttc gtcgacgccg ggggcaccct cgtcgagacg gccgacggct acggcgacgg
    19081 cgccgcacag gagaccctcg gcgcagtgct cggcgcggcc gtgccgcgcg accacctggt
    19141 catcgcaggc cgtgcgatgt tgcccggtgg cgcactcggc gacggtgccg ctcgcggcgc
    19201 gctgctcgcc gggctcgacg ccacgctcaa gcgcatcggc accgaccacc tcgacctgtg
    19261 gcagctgccc ggttttgatg ccgacgtccc gctggaggag acgctgtccg cggtgcaggt
    19321 ggccgtcatg gcggggaagg cccgctacgc ggggctcgtc gcgccgaagg ggtggcagct
    19381 cgccacggtc gcggagcgcg ggagggcgat gggcggcatc accgtgccgg tgtccgcgca
    19441 ggtggagtac tcgctgctgt cccgggacgt cgaggacgac ctgctcccgg ccgcgcggca
    19501 ccacggcgtc gggctgctgg cctgggcgcc actcgggcgg ggcgtcctca ccggcaagta
    19561 cgcggacggc accccggccg actcgcgggc ggcatcgccg acgctggcgc cctacgtcga
    19621 ggcgcggcgc aacgagcgct ccgcccggat cgtgcaggcc gtgctcacgg cggccgacgg
    19681 gctgggcacc tcaccgctgt ccgtcgcact ggcgtgggtg cgcgaccggc ccggcgtgtc
    19741 cgcagccatc gtcggggccc gcgatgccgc acagctgacg gcgtcgatgg cggccgattc
    19801 ggtggtgctg ccggcggaga tccgctctgc tctcgacgac gtcagcggcc cgggatgaca
    19861 atcgaggcgg ccgcccgcat ctggcaccgc ggtgcgaccg cgcctatggt gttggggatt
    19921 ccacccggcg ttcaccgcta tgtggggtgg caggtggacg agctggcggg aggtgagccg
    19981 gtggaccgaa gtggcccgac cggcacggtc gagggggatt cgatcgacgg ggaatgggag
    20041 taccgcccgg tccagctacc cggtgatgtg tcccggatga ccgcggcggt gcgactggcg
    20101 atccaggccg agttcggcgg ctgggagctc tcgcgcgtgc ggctctaccc gggtggcgtc
    20161 cgcaaggtgt ggctgcgccg gcgtcgcagc tcgcgaatgc tcccggagct ctccgtctag
    20221 tcctgctagt cctggtctcg tcagtggtgc tcccacaaca ccccggttgg cggaggtgtg
    20281 ccgtccttcg ggccgggcac cccgcggaaa ccgtagggcg ccggttcacg gccgggctgc
    20341 acgaaccagc actcccccgg ttccccgcgc tcgagaatcg tctcgatcgc cgcggcgacg
    20401 tcgtccgcgg cgagcagcgg gaagtcgccg aaccgggact tcgcgttcgc gatcagcggt
    20461 gtgtcggcga agccggggca cacggcgttc acccggatcc cgtccgcaac cagcgtcggc
    20521 gccgccgcgc ggacgtaccc caccacggcg tgcttcgtca tcgtgtacag cgcgtcacca
    20581 ggcatggcga ccagcccggc cagcgaggcc gtcgcgacga tcgtgccgcc acctgccctc
    20641 cgcaacgccg gcacggccgc cgtcaggccg aacaccacgt ggtcgacgtt gacgcccacg
    20701 atcctgcggt acgcggcaag gtcgaggttc tcgacgcccg actggcctgc ggtgaccccg
    20761 gcgttcagca gcacgatgtt gagccgcccg aacgcatcct cggccacgtc cacggccatc
    20821 gtcatgatgt cgggatccga ggcgtctccg ggcagcgcga gcccgccgac ctcctcggcg
    20881 agcgcccggg cgcccgcctc gtcccggtcg gcgacggcca cgacggctcc tgcggcggcg
    20941 agccgccgca ccgcggccgc cccgatcccc gatgcccctc cggtcaccag tgctgctttg
    21001 cccgtgagat ccatcaacag ctcctcagga atgtgtcgag cacgcggcaa ccgaacttca
    21061 gggcgtcgat gggcacgcgc tcgtcgatcc cgtggaacag ggacgcgaag tccaggtcgg
    21121 gcggcagccg cagcggcgcg aagccgtagc agttcatgcc gaggtcggcg aaggacttcg
    21181 cgtcggtgcc gccggagagc atgtacggca ctgtcgccgc cccggggtcc tccgtgcgca
    21241 acgcggcggc catcgcgtcg gtgagcggcc cgccgaacgg cgtctcgatc gcgggcaggt
    21301 gcgtgatcca ctcccggccg acgtccgggc cgagcagctc gtccacctcc cggaggaacg
    21361 catcctcgcg ccccggcagg acgcggcagt cgagcaccgc ctccgcggac gagggaatga
    21421 cgttcgcctt gtagcccgcc gagagcatcg tggggttcgc ggtgtcgcgg atggtggcgc
    21481 cgacgatccg cgagagcggc ccgagcttgg cgagtgaccc ctcgaggtcg tcctccggga
    21541 actgcagtcc ggtgagctcg gccatctgct cgaggaacgc gcggacggtg tcggtgagca
    21601 ccagggggaa cgtgtggttg cccaggcgcg cgacggcctc ggcgaccttg gtgacggcgt
    21661 tgtcgtcgtg gaggaacgac ccgtggcccg gcttcccgcg agcgcgcagc cgcatccacg
    21721 agaggccctt ttccgcggcc tcgatgaggt agacgcgacg gtcctcggca agcgtcaggg
    21781 agaacccgcc cacctcgccg acggcctcgg tgcacccctc gaacaggtcg ggccggttgg
    21841 cggcgagcca ctgggcgccg tccttgccgc ccgcctcctc gtcggccacg aaggcgaaga
    21901 cgatgtcccg gggcggcacg acgccgtcgc gcttgaaccg acgcgcgacc gcgatcgtca
    21961 tcgcgaccat gtccttcatg tcgaccgcgc cgcggcccca cacgtacccg tcctgcacgg
    22021 cacccgagaa cgggtgcacc gaccactcgg tcgggtcggc gggcaccacg tcgaggtggc
    22081 cgtgcaccag caacgctccc cggtccgggt cggcgccctt cagccggcag atcacgttgt
    22141 cgcgccgcgg cgcgccggac tcgaccacct cgacctcgta gccgacgtcg gagagcttgg
    22201 ccgccacgta ctccgcggcc tcgcgctccc ccaccacggt gtcggggtcg ccggtgttgg
    22261 tcgtgtcgat ccggatgagc tccgaagcca gctcgacgac ctcgtcctcg gctgccacgc
    22321 ttcgtcccat cgcatccctg gtcacgccga tcttcctacc accggaggtc ccggcgcggg
    22381 cgcccggcgc gcgtcggcta tcctcatctc cgcacacccg agagggtgcg cagcatgtcc
    22441 gagtggcgga atggcagacg cgctagcttg aggtgctagt gccctttaac gggcgtgggg
    22501 gttcaagtcc cccctcggac accgggagaa ggccctgttc aggggtcttt tttccgtttc
    22561 cgggcttgtc ggtgcagggc tacgcttcag gagtcgtacg ccggccggga gggccggttt
    22621 cccatctccg cctcgggagc ccgcatgacg tccacccacg gctcgactcc tgccaccgac
    22681 taccccgaga acatcgacgc ggccgtcctc aagaccgctg gc
//
LOCUS       DASRSC010000020         7959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1970250,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000020 DASRSC010000000
VERSION     DASRSC010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1970250"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1082)
                     /locus_tag="VFE39_00810"
     CDS             complement(<1..1082)
                     /locus_tag="VFE39_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6257167.1"
                     /translation="MTGATLDAARTSLDYPVVPVGALLAGSARRFGDRTAVHFAGREL
                     SYAQLYERACAFANALGAAGVGRGDVVAVHLPNCPQYAIAYYGILLAGATFSPTNPLL
                     PPTDLAAQLADCGAVAAVSWAPAAPALAAVRHSTAIRLILVTDREQALDPAHRVALDG
                     IADALDFEAFHAEAPVTDPAVAVDVYRDLAHLAYTGGTTGRSKGVQVPHRNVVVNSLQ
                     YACWGTGSVPVLDDRGGVVLDQQAPAEEHPVRLGNSVSINLTPWFHAMGTIGGLNVPV
                     LGGGTTVLHERFDPGAYLVDAEKFRVTGMGGAPPLFGALLRHPEFATRDLSSVRGISS
                     GAAPMPVEMIEALHRRFGDDVVIVEG"
     gene            complement(1079..2368)
                     /locus_tag="VFE39_00815"
     CDS             complement(1079..2368)
                     /locus_tag="VFE39_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6257168.1"
                     /translation="MAPTSTAAEGSTWIGRPDLSDIPGFWTLPIAERAAAFAALRERE
                     PVPFFAEPEQSFLPRGAGYWALTKLDDLLVASRNPAVFTSGKGSTSIADFPPEFIEFF
                     GSMIGMDDPRHARLRRIVSRGFTPKRLSALTNEVEQTARSIVDDVIDAGECDAVPAIS
                     ARLPLKIVCDMMGVPESQYEFVYSRSNIILGASDPDYVPDADNFVTALLQAAGELADL
                     MRDLGRLRTANPRDDVTSALVNAEVDGERLTPDELASFFILLVVAGNETTRNAISWGL
                     QLLTDHPDQRAAWLADIDGVTPTAVEEIVRLASPVIFMRRTLASDAVLGGQQMSAGDK
                     VLLFYWAANRDPAHFTDPDRFDVRRSPNPHVGFGGPGPHFCLGAHLARREMSVMFREL
                     LTRVPDIHATAPPERLRSMFINGIKRLPVAFTPGGAR"
     gene            complement(2434..5505)
                     /locus_tag="VFE39_00820"
     CDS             complement(2434..5505)
                     /locus_tag="VFE39_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018681081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6257169.1"
                     /translation="MTGVARLGVGIGLVGRRHEVSALTAALDRAVACRPTGVLLSGDA
                     GVGKSRLVTEAVERARTAGFTVLLGRCLDAAESSLPYLPFTEIVGALAAVHPELVGEH
                     VALRHLLPGGYPRGPQSGEDRDLGQLRVFDAVLSVLDELTTTAPVLLVVEDLHWADRS
                     SRDLLIFLLSRLSGQRLVVLATYRSDDLHRRHPLRPVLSELVRLPAVERVEVAPLDAE
                     RALELVRLLADGNLPEPMLHNLARRSEGNAFFAEELVSASSDGLPHGLAEVLLARIEG
                     LSAATQRVLRIASVAGRRVQHDRLAVVSGLPVDELEQALREAVTHHVLVAAGSEPPGI
                     STDDAYVFRHALLREAIYHELLPGERSRLHAAYAALLADQSRGGPERAAEPGHAAELA
                     HHAMAGHDLSLALAASVQAANEANDREAPAEMLLHAERAIELWPAVPDAEAVAGVDEG
                     TITRWAAWGASATGDPDRGIALGRRALELADQRGDPMRTAAIARRYALRLLDLSGREQ
                     EALEVARRALQLVADAPPSATLAWGHAVLARVLGTLDHFTEAVLQAETALEVARRLPP
                     DDLSAVGATADALVTLAVCEEYGGNPVRARQRLGEAKPLARRSGNLSVELRAYFAVGI
                     SLLDEGRLAEAGDEFAAGEERAAATGTTWGGYGLECRVAHVIARFLRGDWDDAEAAAE
                     LAGESVSATVASRLTAAGLLTSVARGRFDAAARRLVELGDSSPVDDQVIMLMGEAGAE
                     AALWQSRPAEAAEHVTAALAGINSGPHAGQAPHLGGIMLAALGVSAQADLAAAGQVPV
                     AEAAEAARMLVATAEHTEAHGLPRAGTLGPEGRAWLCRARAELTRIAGPPDPSAWDSL
                     VTAFGYETGAHPGEPPAAGSYGYRQAYALLRRAEALLEGGAPHGQVEPDLRAALRTAE
                     RLSARPLAEAVRTLAARAGVRLDPTHVRPEPVGPGPLTPRERSVLALVAAGHTNRQVG
                     AELFISEKTVSVHLSRVMAKLGASSRTEAVSVAYTRGLLAPADREMARADP"
     gene            complement(5502..5741)
                     /locus_tag="VFE39_00825"
     CDS             complement(5502..5741)
                     /locus_tag="VFE39_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257170.1"
                     /translation="MLINFVHTAADADHHRRALLADAANFRLAKLAKAARRARRAERS
                     PMGTGSAPGADPSYRARQNPAQRNDDAERRCTVSP"
     gene            6002..6166
                     /gene="rpmG"
                     /locus_tag="VFE39_00830"
     CDS             6002..6166
                     /gene="rpmG"
                     /locus_tag="VFE39_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET6257171.1"
                     /translation="MARNQLRPIVRMRSTAGTGTTYVTRKNRRNDPDRMVLRKYDPAA
                     RRHVEFREER"
     gene            6290..6362
                     /locus_tag="VFE39_00835"
     tRNA            6290..6362
                     /locus_tag="VFE39_00835"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6323..6325,aa:Thr,seq:ggt)
     gene            6391..6463
                     /locus_tag="VFE39_00840"
     tRNA            6391..6463
                     /locus_tag="VFE39_00840"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6424..6426,aa:Met,seq:cat)
     gene            6498..6665
                     /gene="rpmG"
                     /locus_tag="VFE39_00845"
     CDS             6498..6665
                     /gene="rpmG"
                     /locus_tag="VFE39_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018598675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET6257172.1"
                     /translation="MAKATDVRPKITLACEQCKHRNYITKKNRRNDPDRLSIKKFCPN
                     CGVHREHRETR"
     gene            6673..7119
                     /locus_tag="VFE39_00850"
     CDS             6673..7119
                     /locus_tag="VFE39_00850"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HET6257173.1"
                     /translation="MPDVSFVGRALPLGVPYRVGREKIREFALAIGEGASVCHDVAAA
                     QAAGHPDLVAPPTFTVTFTMPAIEVFLRDPAFGWDYARMVHGDQSITLHRPIHAGDDL
                     VTTIHVEDLTTRGGSHMLRLRCEVADSEGAPVATTRALLVTQAETS"
     gene            7116..7553
                     /locus_tag="VFE39_00855"
     CDS             7116..7553
                     /locus_tag="VFE39_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET6257174.1"
                     /translation="MTDTGAVAVGDELPPLSLRVTRADLVRYAGASGDFNPIHWSDRV
                     ATGVGLPGVIAHGMLTMALAGRLVTEWVGDPGAICTYGVRFTRPVVVPDDDEGALVEL
                     SGTVSELTEADDGGQVARLTITARFDGRTVLSRAVAEVHLPSR"
     gene            7596..7898
                     /locus_tag="VFE39_00860"
     CDS             7596..7898
                     /locus_tag="VFE39_00860"
                     /inference="COORDINATES: protein motif:HMM:NF040083.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6400 family protein"
                     /protein_id="HET6257175.1"
                     /translation="MPAPRVLLDRMNPTNEGEPGQLERLRPLPLPPGDGLASHTFDLG
                     AHEQRRLAAVLAATRDLDPGAVLAAEAAAHRMLYSGLDAEQQATYRMLVDAGVLDA"
BASE COUNT         1140 a         2834 c         2921 g         1064 t
ORIGIN      
        1 tagccctcga cgatcacgac atcgtcgccg aagcggcggt gcagcgcctc gatcatctcc
       61 accggcatcg gcgccgcgcc cgaggagatg cctcgcaccg acgacaggtc gcgcgtggcg
      121 aactcgggat ggcgcagcag cgcgccgaac agcggcggcg ctccgcccat gccggtgacg
      181 cggaacttct cggcgtcgac caggtaggcg cccgggtcga accgctcgtg cagcaccgtg
      241 gtgcctccgc ccagcacggg aacgttgagg ccgccgatcg tgcccatggc gtggaaccag
      301 ggcgtgaggt tgatgctcac gctgttgccc agccgcacgg ggtgctcctc ggcaggcgcc
      361 tgctggtcga gcacgacgcc gccgcggtcg tccaggacag ggaccgagcc ggttccccag
      421 catgcgtact ggagcgagtt gaccacgacg ttgcggtgcg gcacctgcac acccttggac
      481 cggccggtcg tgccgccggt gtaggccagg tgggccagat cgcggtagac gtcgacggca
      541 acggcagggt cggtgacggg cgcctcggca tggaaggcct cgaagtccag tgcgtccgcg
      601 atgccgtcga gcgccacccg gtgcgccggg tcgagcgcct gctcgcggtc ggtgaccagg
      661 atcagcctga tcgccgtgct gtgccgcacc gcggcgagcg cgggagctgc cggcgcccag
      721 ctgaccgcgg cgaccgcgcc gcagtcggcc agctgtgcgg cgaggtcggt gggcgggagc
      781 agcgggttcg tcggcgagaa cgtggctccg gccagcagga tgccgtagta ggcgatggcg
      841 tactggggac agttcgggag gtgcacggcc accacatcgc cgcgcccgac gccggccgcc
      901 cccagtgcgt tcgcgaaggc gcacgcccgc tcgtagagct gcgcgtacga cagctcacgg
      961 ccggcgaaat gcaccgcggt gcggtcgccg aagcgacgtg cggagcctgc gagcaacgca
     1021 ccgaccggga ccacggggta gtccagcgag gtgcgggcgg catcgagggt ggcgccggtc
     1081 atcgggcgcc accgggggtg aacgcgaccg gcaggcgctt gatgccgttg atgaacatcg
     1141 accgcagccg ttccggtggc gccgtggcgt ggatgtcggg gacgcgggtg agcaactcgc
     1201 ggaacatcac ggacatctcg cggcgcgcca gatgcgcccc aagacagaag tgtgggccgg
     1261 ggccgccgaa cccgacgtgc ggattgggcg agcgccggac gtcgaacctg tcgggatcgg
     1321 tgaagtgcgc ggggtcgcgg ttggccgccc agtagaacag cagcaccttg tcgcctgcgc
     1381 tcatctgctg gccgccgagc accgcatcgg acgccagggt gcggcgcatg aagatcaccg
     1441 gggaggccag gcggacgatc tcctccaccg ccgtgggggt gacgccgtcg atgtcggcga
     1501 gccacgcggc gcgctggtcg gggtggtcgg tcagcagctg caggccccag ctgatggcgt
     1561 tgcgggtggt ctcgttgccc gccaccacga ggaggatgaa gaaggaggcg agctcgtcgg
     1621 gcgtgagccg ctcgccgtcg acctcggcat tgaccagcgc cgaggtgacg tcgtccctgg
     1681 ggttggcggt gcgcagcctg cccaggtcgc gcatcaggtc ggctagctcg cctgctgcct
     1741 gcaggagcgc cgtgacgaag ttgtcggcgt cgggcacgta gtcggggtcg ctggcgccga
     1801 ggatgatgtt ggaacggctg tagacgaact cgtactggct ctccggcacc cccatcatgt
     1861 cgcagacgat cttcagtggc agccgggcgg agatcgccgg gacggcgtcg cactcgcccg
     1921 cgtcgatgac gtcgtcgacg atggagcgcg cggtctgctc cacctcgttg gtgagcgcgc
     1981 tgagccgctt cggggtgaat ccgcgggaga cgatccgccg gagccgggcg tggcgcgggt
     2041 cgtccatgcc gatcatcgac ccgaagaact cgatgaactc gggcgggaag tcggcgatcg
     2101 aggtggagcc cttgcccgag gtgaagaccg cggggttacg gctggccacc aggaggtcat
     2161 cgagcttggt cagtgcccag tagccggccc cccgcgggag gaagctctgc tccggctcgg
     2221 cgaagaacgg aaccggctcc cgctcccgca gcgccgcaaa ggccgcggcc cgctctgcga
     2281 tcggcagggt ccagaagccg gggatgtcgg acaggtccgg ccggccgatc caggtgctgc
     2341 cctcggcggc ggtgctcgtg ggtgccatcg ctgagcctcc ttgcgggtgg ggtgaacccg
     2401 atcacccaca ccctagagat caccgggatc ggctcaaggg tccgcccgcg ccatctcgcg
     2461 gtccgccggg gccagcaggc cgcgggtgta cgcgacgctg accgcctcgg tacggctgct
     2521 cgctcccagc ttcgccatga cccgggacag gtggacgctc acggtcttct cgctgatgaa
     2581 cagctcggcg ccgacctggc ggttggtgtg cccggccgcc accagcgcga gtacggaccg
     2641 ctcccgcggc gtgagcggcc cgggcccgac cggctccgga cgcacgtgcg tggggtcgag
     2701 tcgtaccccg gccctcgcgg ccagtgtgcg gacggcctcg gcgagcggcc gtgccgagag
     2761 ccgttctgcg gtgcggagcg cggcacggag atcgggctcg acctgcccat gcggggcgcc
     2821 cccctccagc agggcctcgg cccggcgcag cagcgcgtag gcctgccggt agccgtagct
     2881 ccccgcggcc ggcggttcgc cggggtgcgc gcccgtctcg tagccgaagg cggtgacgag
     2941 gctgtcccac gccgacgggt cgggcggccc cgcaatccgg gtgagctccg cccgcgcccg
     3001 gcacagccag gccctgccct ccggaccgag ggtgcccgca cgcggaagac cgtgggcctc
     3061 cgtgtgctcg gcggtggcga cgagcatgcg ggccgcctcg gccgcctcgg caaccggaac
     3121 ctggcctgcg gcggcgaggt cggcctgcgc ggacacaccc aacgcggcca gcatgatccc
     3181 gcccaggtgc ggcgcctgcc cggcgtgcgg cccactgttg atcccggcga gcgccgccgt
     3241 gacatgctcc gccgcctcgg caggcctgct ctgccagagc gcggcctccg cccctgcctc
     3301 gcccatcaac atgatcacct ggtcgtcgac gggcgacgag tcgcccagct cgaccagccg
     3361 ccttgcggca gcatcgaagc gaccacgcgc cacggaggtg agcaggcccg cagccgtcag
     3421 cctgctcgcg acggtggccg acaccgactc gcccgcgagc tcggccgcgg cctccgcgtc
     3481 gtcccagtcg ccgcgcagga accgggcgat gacgtgagcg acccggcact ccaggccata
     3541 gccgccccat gtggtgccgg tggcggcggc gcgctcctcg cctgctgcga actcgtcgcc
     3601 cgcctcggcc agcctgccct cgtcgagcag cgagatgcct acggcgaaat aggcgcgcag
     3661 ctccacgctg aggttaccgg agcgtctggc tagcggcttg gcctcgccga gccgctggcg
     3721 agcccgcacg gggttgccgc cgtactcctc gcacaccgcg agcgtgacga gcgcgtcggc
     3781 ggtggctccg actgcgctga gatcgtcggg cggcaggcgg cgcgcgacct ccagagcagt
     3841 ttcggcctgc agcacggcct cggtgaagtg gtcgagcgtg cccagcacgc gtgccagcac
     3901 cgcgtgcccc caggcgaggg tggccgacgg cggggcgtcg gcgaccagct ggagggcgcg
     3961 ccgggccacc tcgagcgcct cctgctcccg gccgctcagg tccagcaggc gcagggcgta
     4021 ccggcgcgcg atcgccgcgg tgcgcatcgg gtcgccgcgc tggtcggcga gctccagggc
     4081 gcgccgtccg agggcgattc cgcgatccgg atcgccggtg gcgctcgcac cccatgcggc
     4141 ccagcgagtg atggtgccct cgtcgacccc ggcgaccgcc tcggcatcag gtacggcagg
     4201 ccacagctcg atcgcacgct ccgcgtgcag cagcatctcc gcaggcgcct cgcggtcgtt
     4261 ggcctcgttg gccgcctgca cggacgcggc gagcgcgagc gagaggtcgt gccccgccat
     4321 cgcgtggtgg gcgagctcgg cggcgtgacc gggctcggct gcccgctcgg ggccgccgcg
     4381 gctctggtcg gcgagcagtg ccgcgtaggc cgcgtgcaga cggctgcgct cgccgggcag
     4441 cagctcgtgg tagatcgcct cgcgcagcag cgcgtggcgg aacacgtagg cgtcgtcggt
     4501 gctgatcccc gggggttcgc tgcccgcggc caccagaacg tggtgggtga cggcctcgcg
     4561 cagcgcctgc tccagctcgt cgaccgggag ccccgacacc acggccagcc ggtcgtgctg
     4621 gacccgtcgc cccgcgaccg acgcgatgcg cagcacccgc tgggtggcgg cggagaggcc
     4681 ctcgatgcgc gccagcagga cctccgcgag gccgtgcggc agcccgtcgg agctcgccga
     4741 caccagctcc tcggcgaaga acgcgttgcc ctcgctacgg cgcgcgaggt tgtggagcat
     4801 cggctcgggc aggttgccgt cggcgagcag ccggacgagt tcgagggccc gctccgcgtc
     4861 gagcggggcg acctcgacac gctccacggc cggcagccgc accagctccg acagcacagg
     4921 gcgcagcggg tggcgccggt gcaggtcgtc gctgcggtag gtggccagca cgacgaggcg
     4981 ctgcccggag agccgggaca gcaggaagat cagcaggtcg cggctcgagc ggtcggccca
     5041 gtggaggtcc tccaccacga gcaggaccgg tgcggtggtg gtgagctcgt cgagcaccga
     5101 cagcaccgcg tcgaacaccc gcagctggcc caggtcgcgg tcctcgccgc tctgcggacc
     5161 gcgcgggtag ccgcctggca acaggtggcg cagggccacg tgctcgccga cgagctcggg
     5221 gtgcacggcc gcgagcgcgc cgacgatctc ggtgaacggc aggtacggca gcgacgactc
     5281 ggcggcgtcg aggcagcggc cgagcagcac ggtgaacccc gccgtccgcg cgcgctcgac
     5341 cgcctccgtc accagccggg acttgcccac accggcatcc cccgacaaca gcaccccggt
     5401 gggccggcag gcaacggcgc ggtcgagcgc ggcggtgagc gccgaaacct cgtgccggcg
     5461 tcccaccagc ccgatcccga cccccagccg cgccacgccg gtcatggtga cacggtgcac
     5521 cgacgttctg cgtcgtcatt gcgctgcgca ggattctgcc gagcgcgata cgacggatcg
     5581 gctcccggtg cggatcccgt gcccatcggt gacctctcgg cccgtcgggc gcggcgcgcg
     5641 gccttggcca gcttggcgag gcggaagttc gcggcgtcgg cgagcagggc ccggcggtgg
     5701 tggtcggcgt ctgctgcggt gtgcacgaag ttgatgagca tgagtagagc ctcgggctga
     5761 ggtggggggc gcggcatcgg acgatcacgt cgtctcggga ccgcggtgcg cctgccccat
     5821 ggggtaaggc cccgcaccct tactccgcga tcgcagcctc caccccaggc gtgtgcagcc
     5881 cggtgagtac caggagctcg cgtcgggtca cgggcgtccc acatgggtcg acggggatgg
     5941 tttcgcggtg cgccagcgtt agctcaacgc aaatcgatgt cgtcaaggac aaggagtggg
     6001 catggctcgc aaccagctgc ggccaatcgt gcggatgcgg tcgaccgcag gcaccggcac
     6061 gacgtacgtg acccgcaaga accggcgcaa cgacccggac cggatggtgc tgcgcaagta
     6121 cgacccggct gcccggcgcc acgtcgagtt ccgcgaggag cgctgacgtg tccggaaagc
     6181 cgcagcgcac gttcgtccag cccgatggcg ggaccggtgt acaggcacgc acgggcgttg
     6241 tgtagcatcg tctccggccg ttcgggaccg accgaccgcg cggcaagccg cccctttagc
     6301 tcagtcggca gagcgtctcc atggtaagga gaaggtctac ggttcgattc cgtaaagggg
     6361 ctcagcgagc tggtgtgacc agcttcgcga agcggtgtag ctcagtcggt agagcaagcg
     6421 gctcataatc gctgtgtcgc cggttcaagt ccggccaccg ctacagaccg accagagaaa
     6481 cgcaggaggc acccgcgatg gccaaggcca ccgacgtccg gccgaagatc acgctggcgt
     6541 gcgagcagtg caagcaccgg aactacatca ccaagaagaa ccggcgcaac gaccccgatc
     6601 ggctcagcat caagaagttc tgcccgaact gcggggtcca ccgcgagcac cgcgagaccc
     6661 gctgagctga gcgtgccgga cgtctcgttc gtcggccggg cgttgcccct aggggttccg
     6721 taccgcgtcg gtcgggagaa gatcagggag ttcgccctcg ccatcggcga gggcgcttct
     6781 gtgtgtcacg acgtggccgc ggcacaggcg gccggccacc cggacctcgt cgccccgccc
     6841 acgttcaccg tcaccttcac gatgccggcg atcgaggtgt tcctgcgcga cccggctttc
     6901 ggctgggact acgcccggat ggtgcacggt gaccagtcga tcacgctgca ccgcccgatc
     6961 cacgcaggtg acgacctcgt cacgacgatc cacgtcgagg acctcaccac gcgcggtggc
     7021 agccacatgc tgaggctgcg ctgcgaggtg gccgacagcg agggcgcccc ggtggccacc
     7081 acccgcgccc tgctcgtcac ccaggcggag acgtcatgac cgacacgggc gctgtcgccg
     7141 tcggcgacga gctgccgccc ctgtcgctgc gggtcacccg cgccgacctg gtgcgctatg
     7201 caggcgcgtc cggcgacttc aaccccatcc actggagcga ccgcgtcgcc accggcgtcg
     7261 ggctgccggg cgtcatcgcg cacgggatgc tgacgatggc cctggccggc aggctcgtca
     7321 ccgagtgggt gggcgatccc ggcgcgatct gcacctacgg agtgcgcttc acccgtcccg
     7381 tcgtcgttcc tgacgacgac gagggagcgc tggtcgagct gtccggcacc gtctccgagc
     7441 tcaccgaggc cgacgacggc ggtcaggtcg cccggctgac catcaccgcg cggttcgacg
     7501 gtcggacggt gctgagccgg gctgtcgcgg aggttcacct cccgtcgcgg tgatgcacct
     7561 cacccgcgtg aggtaaatcc cgaatcctgg ggagcgtgcc cgcccctcgg gtactcctgg
     7621 accggatgaa cccgacgaac gaaggcgagc ccggccagct ggagcggctc cgtcccctcc
     7681 cgctcccacc cggcgacggt ctcgcgtcgc acacgttcga tctcggcgcg cacgagcagc
     7741 gcaggctcgc cgcggtactc gcggcgaccc gggacctgga ccccggtgcc gtgctggccg
     7801 ccgaggcggc ggcccaccgc atgctctaca gcggcctcga cgccgagcag caggcgacct
     7861 accggatgct cgtcgacgcc ggggtcctcg atgcgtgacg cccaggccga catcggccgc
     7921 cgggtgtgga cgacgtgccc gcgctgcatc gacccgagc
//
LOCUS       DASRSC010000021         2660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3281386,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000021 DASRSC010000000
VERSION     DASRSC010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3281386"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1276
                     /gene="purF"
                     /locus_tag="VFE39_00865"
     CDS             <1..1276
                     /gene="purF"
                     /locus_tag="VFE39_00865"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET6257176.1"
                     /translation="STTWENAQPSFRTTPAGTGIALGHNGNLVNTAELAGRHRADPPR
                     NDGRRPVTATTDSDIVTGLLADSAADLGVEEAALRLLPDLKGAFSLVFTDETTLYAAR
                     DRHGVRPLVLGRLDRGWVVASETAALDIVGASFVREVEPGELIAVDAEGLRSSRFAPP
                     EPKGCVFEYVYLARPDTTIAGRGVHSARVEIGRRLAVEHPSEADLVIPVPESGTPAAI
                     GYAEGSGLPYGQGLVKNAYVGRTFIQPSQTIRQLGIRLKLNPLREIIRGKRLVVVDDS
                     IVRGNTQRSLIRMLREAGALEVHVRIASPPVRWPCFYGIDFASRAELIANGLDDEGIR
                     RSIGADSLGYISADGLVAATEQPRSRLCTACFSGEYPIPLPDEAMVGKHLLENVTGAS
                     CGDGGRLASPVVHEGVSVAAPGYGAEDALSRP"
     gene            1392..2528
                     /gene="purM"
                     /locus_tag="VFE39_00870"
     CDS             1392..2528
                     /gene="purM"
                     /locus_tag="VFE39_00870"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET6257177.1"
                     /translation="MSTDAGSEPSPPAELPSTGPASYAAAGVDVEAGERAVDRLKPLA
                     ASTTRPEVLGGLGGFAGLFALRAGRYREPVLAASTDGVGTKVAVAQAMDRHDTVGLDL
                     VAMVVDDLVVCGAEPLFLQDYLAIGKLVPEKVEQIVAGIAEGCRQAGCALLGGETAEH
                     PGVMEPHHYDLSATGVGIVEADRTLGPDRVRPGDTVVAMGSSGLHSNGYSLARQVLLQ
                     IGRMSLDGQVEEFGRTLGEELLEPTRIYARDCLALAAETDVRTFAHVTGGGLCANLAR
                     VLPPGLVAELDRTTWTPAAVFKLIATRGRVTRDEMERTFNMGVGMVAILPPEDVDRAM
                     AVLTARHVPSWVIGEVRRPGPEEALPEPAPRAGAAVLRGDHPRF"
BASE COUNT          344 a         1013 c          952 g          351 t
ORIGIN      
        1 gtccacgacc tgggagaacg cgcagccgag tttccgcacc acgccggccg gcaccgggat
       61 cgccctgggg cacaacggca acctcgtcaa caccgccgag ctggccgggc ggcaccgggc
      121 cgacccgccc cgcaacgacg gccgccggcc cgtcaccgcg accaccgact ccgacatcgt
      181 cacgggcctg ctcgccgact ccgccgccga tctcggcgtg gaggaggcgg cgctgcggct
      241 gctgcccgac ctgaagggcg cgttcagcct ggtcttcacc gacgagacca cgctgtacgc
      301 cgcccgcgac cgccacggcg tgcgcccgct ggtgctgggc cggctcgacc gcggctgggt
      361 ggtggcctcg gagaccgcgg cgctcgacat cgtcggtgcc tcgttcgtcc gcgaggtcga
      421 accgggtgag ctgatcgccg tcgacgccga ggggctgcgc agctcgcggt tcgcgccccc
      481 ggaacccaag ggctgcgtgt tcgagtacgt ctacctcgcc cgccccgaca cgacgatcgc
      541 cggccgcggc gtgcactcgg cgcgggtgga gatcgggcgc cggctcgccg tcgagcaccc
      601 gtcggaggcc gacctggtca tccccgtccc ggagtcgggg acgccggcgg cgatcgggta
      661 cgccgaggga tccgggctcc cgtacggcca gggactggtg aagaacgcct acgtcggccg
      721 gacgttcatc cagccgtcgc agaccatccg gcagctcggc atccggctca agctcaaccc
      781 gctgcgcgag atcatccgcg gcaagcgcct cgtcgtggtc gacgactcga tcgtccgcgg
      841 caacacgcag cgctcgctga tccggatgct ccgcgaggcg ggggcgctcg aggtgcacgt
      901 gcggatcgcc agcccgcccg tgcgctggcc ctgcttctac ggcatcgact tcgcctcgcg
      961 cgccgagctg atcgcgaacg gcctcgacga cgagggcatc cgccgctcga tcggtgccga
     1021 ctcgctgggc tacatctccg ccgacggcct cgtcgcggcc accgagcagc cccgctcgcg
     1081 gctgtgcacg gcctgcttct ccggcgagta cccgatcccg ctgcccgacg aggcgatggt
     1141 gggcaagcac ctcctcgaga acgtcaccgg cgcgagctgc ggcgacgggg gccgcctggc
     1201 gtcgccggtg gtgcacgaag gggtatcggt ggccgcgccg ggatacggtg ccgaggacgc
     1261 gctgagccgt ccctgacggc acgacgtcac cgggccacgc cgcccacccg tcatccggtc
     1321 acccgacccg acccgagccg ccccgggccg ccgcgcggtc cgacgcaccc accttcgagg
     1381 actccccacc gatgagcacc gacgccggtt ccgagccgtc ccccccggct gagctcccct
     1441 cgaccggtcc ggccagctac gccgccgccg gcgtggacgt cgaggcgggg gagcgcgcgg
     1501 tcgaccggct caagccgttg gcggcctcga cgacccgtcc cgaggtgctg ggcggcctcg
     1561 gcggcttcgc ggggctcttc gcgctgcggg cgggccgcta ccgggagccg gtgctggccg
     1621 cgtccaccga cggcgtcggc accaaggtcg cggtggcgca ggcgatggac cgccacgaca
     1681 cggtcggtct cgacctggtg gccatggtcg tcgacgacct cgtggtgtgc ggggccgaac
     1741 cgctgttcct gcaggactac ctggccatcg ggaagctcgt gccggagaag gtcgagcaga
     1801 tcgtcgcggg catcgccgag ggctgccgtc aggcgggatg tgcgctgctc ggtggcgaga
     1861 ccgcggagca ccccggcgtg atggagccgc accactacga cctctccgcg accggcgtcg
     1921 gcatcgtcga ggccgaccgc acgctggggc ccgaccgcgt gcgccccggt gacactgtcg
     1981 tcgcgatggg ctcgtccggc ctgcactcca acggctactc gctggcccgc caggtgctgc
     2041 tgcagatcgg gcgcatgtcg ctcgacgggc aggtcgagga gttcggccgc acgctcggcg
     2101 aggagctgct cgagcccacc cgcatctacg cgcgcgactg cctggcgctc gccgccgaga
     2161 ccgatgtccg cacattcgcg cacgtcaccg gcggtggcct gtgcgccaac ctggcgcggg
     2221 tgctgccccc gggcctcgtc gccgagctcg atcgcaccac ctggaccccg gcggcggtgt
     2281 tcaagctcat cgcgacccgg ggacgggtga cgcgcgacga gatggagcgc accttcaaca
     2341 tgggcgtcgg gatggtggcg atcctcccgc cggaggacgt cgaccgtgcc atggccgtgc
     2401 tcaccgcgcg ccacgtgccg tcgtgggtca tcggcgaggt gcgtcggccg ggccccgagg
     2461 aggccctccc cgagcctgcg ccacgggccg gtgcggccgt cctgcggggc gaccacccga
     2521 ggttctagtc cggcatcagc gtgctgcggg gcgtgagcac cgcccggccg tcggcctgcg
     2581 tcaccacgga ggccgcgtcg cccctgcggg acgccgtcgt ggatacagca agcacccgct
     2641 ctcctcgatc acgtgagaaa
//
LOCUS       DASRSC010000022         8876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2953584,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000022 DASRSC010000000
VERSION     DASRSC010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8876
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2953584"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VFE39_00875"
     CDS             complement(<1..488)
                     /locus_tag="VFE39_00875"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET6257178.1"
                     /translation="MPIATPEKYNEMLDKAKSGEYAYPAINVTSTETLNAALRGFAEA
                     ESDGIVQISTGGAEFLSGTKVKDMVTGAVGLAEFAHVVAAKYPINVALHTDHCPKDKL
                     DSYVRPLIAISQERVDRGENPLFQSHMWDGSATELHENLRIAAELLDQAVKAKIILEV
                     EIG"
     gene            593..1177
                     /locus_tag="VFE39_00880"
     CDS             593..1177
                     /locus_tag="VFE39_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HET6257179.1"
                     /translation="MADDGGGNAVEQTLSRLRRIDTRTEPAPAPGAPRTLSDLYRDHR
                     MRMVRLAVLLVDDPSTAEDVVQEAFTGLHRHWSGLRDEAAAIGYLRTAVVNGSRSVLR
                     RRRTARDYVPPHQVTARSAESLALLSAEHQAVVDALATLPPRQREVLVLRYYGGLSEA
                     EIADATGISRGTVKSTASRALEAVARVMSTGGAR"
     gene            1183..1407
                     /locus_tag="VFE39_00885"
     CDS             1183..1407
                     /locus_tag="VFE39_00885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257180.1"
                     /translation="MSTSDDSERRVAEALRARASGAGRAGARRTAPPMPPAARGVQTA
                     LLIGLLGGIVLGIVLALLSLLTPGVLPALG"
     gene            1436..2080
                     /locus_tag="VFE39_00890"
     CDS             1436..2080
                     /locus_tag="VFE39_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET6257181.1"
                     /translation="MTALALGPEWLQPDSIIAWLGPWALVGLALIVFAECGLLLGFFM
                     PGDSLLFTAGLFVAQGAIQVPLWLVCAILVVAAFAGNTAGYWIGRAAGPAVFDKPKSR
                     LFKPQHVRKTQDFFDKYGNRAIVLGRFVPVVRTFITVMAGVGRMEPKRYLTYSLIGGV
                     AWAAGVTVLGFWLGQFVFVRANIELMLILIVLVSVLPILVELVRARRSGGAHAK"
     gene            2129..2827
                     /locus_tag="VFE39_00895"
     CDS             2129..2827
                     /locus_tag="VFE39_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET6257182.1"
                     /translation="MSPTSTLDHAVGPLAEAGPVTVWIVVLAFVFLECAFIVGMFLPG
                     DTLLLAAGVVLAQSEHEAGAWTLGMVAAAVAVAGNQVGYLVGRYAGTRMLARREGRIL
                     NRANLARAAAFFDRWGFWAIVVARWVPWVRTLAPMIAGAARMDNRRYIVANAVGAICW
                     VPTLIMLGYYGAGLLDVVPWLKTATTIAAVAFFVVGTGYGLYRYHQEMRRPVDDSPAD
                     PVPAGEDQDLRDAG"
     gene            complement(2846..3913)
                     /locus_tag="VFE39_00900"
     CDS             complement(2846..3913)
                     /locus_tag="VFE39_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 76 protein"
                     /protein_id="HET6257183.1"
                     /translation="MAAAEVDWSERADIAERAVVRRHLRRVARVVPGTRIGRVRWPRP
                     LPAPLAPWHYWWQAHLLDCLLDAQQRSPAQRRAHAITALVRGVHLRNAGSWLNRYYDD
                     IAWLGLAVQRAGPLAGRSGPPALAAIMNRLDDGWTEGAGGGIWWRRDDDFKNAPANGP
                     AAILLARDGQLAFAAAIADWMAETLVDPDSGLVQDGVRLASDGSIRAVEPYIYTYCQG
                     VHLGACVELAERDGHQRFTDRAIAIVDAMTHRLADPRGVLPGSGGGDGGLFAGITARY
                     LADAALRVPELAGPAARIVQAGALAVWNGRAEVRGRPVFSAEWGTPAFEPRRGVPEAD
                     LSVQLSGWMVLEAAARLQRSG"
     gene            3992..4987
                     /locus_tag="VFE39_00905"
     CDS             3992..4987
                     /locus_tag="VFE39_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6257184.1"
                     /translation="MPEHDVLIPVFDGIQPLDAAGPHEVLMGATRLLALIGSEPGYRV
                     TFVAAAAAGTVTSDSGMRLCADGPLPESGQIGTLLVPGGSGARGMTPDDPFVGWLRRA
                     ATRSARVVSVCTGAFPLAAAGLLDGLSATTHWRYAHALATAYPAVDVQPDPIYLRQGR
                     IWTAAGVTAGVDLALALVEADHGVDVAQFIARELVVFLRRPGGQSQFAGPVWSPPARQ
                     PSIRAAQDLIHGDPAADLRVPVLAARVGMSERHFSREFTRAIGRPPGDYVEQVRVDVA
                     KRLLESEPVLVTVAAARSGFGTAETMRRAFLRRVGVAPDQYRRYFATASHTAERT"
     gene            4990..5628
                     /locus_tag="VFE39_00910"
     CDS             4990..5628
                     /locus_tag="VFE39_00910"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET6257185.1"
                     /translation="MQVAIALFPRLTALDAIGPYEVLQRVPSLDIVFVGERRGEVRTE
                     NGFLGLTVDATFDEVPAPDVVLVPGGIGSRELLESGKFLDWVRSAHAGTRFTTSVCSG
                     SLALAAAGLLDGLTATTHWGAMEHLGKLGAVPVTQRVVEHLPERVITAAGVSAGIDMG
                     IRLVELLVDAVAAQAAQVYIEYDPQPPFDAGHPSRVTTEVMERVHEHRQVRA"
     gene            complement(6013..6702)
                     /locus_tag="VFE39_00915"
     CDS             complement(6013..6702)
                     /locus_tag="VFE39_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET6257186.1"
                     /translation="MPSRQTLVFDADDTLWENNVLFERVIDAYLDWLAHPTLDRAATR
                     AVLDEVERANVAVHGYGSASFLRSLGDAFERLNERPATPTEVRRIADLAAALVNHEVE
                     LVPGAAETLADLGTRHDLLLLTKGDPAEQQRKIDASSLAHHFRSVHIVAEKRVTTYRE
                     LVAEQGLAPSSTWMIGNSPRSDILPARAAGLGAVFIPNQHTWALEKDELDPDDGGVLH
                     LQRFPELVEHF"
     gene            6790..7233
                     /locus_tag="VFE39_00920"
     CDS             6790..7233
                     /locus_tag="VFE39_00920"
                     /inference="COORDINATES: protein motif:HMM:NF025447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HET6257187.1"
                     /translation="MDTPERFIHQIDQYDAWQRETIRRYGWALQAVLGDGEGPPFVYT
                     VGLSGFGHPELILFATSQATAATVLNDLGELVRAGHGLAAGELIRLRSGDVHLLAFPE
                     SAAWLLAANDLYRIFDGPPVPALLVVPADERVPTPGDGQPCVFCS"
     gene            complement(7245..8045)
                     /locus_tag="VFE39_00925"
     CDS             complement(7245..8045)
                     /locus_tag="VFE39_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET6257188.1"
                     /translation="MSEDPTAAAGNGPAAKGASTRGGRLDQPDWATRAPGAGDSRNGS
                     EGGEGEGAGPSEWAVPGQVGVGPWPGGRDAWPRDEQYDPELLEHGDARNVADAYRYWR
                     RDAVAADLARRAHPFHVAIENFGHDHNIGTVVRTANAFAAAGVHVVGRRRWNRRGAMV
                     TDRYLQVHHHETLSGLVAFAAGHGLAVVAVDNTSGAQRLETADLPRHCVLLFGQEGPG
                     VTGEAHAAATMTVSIAQFGSTRSINAGVAAGIAMHAWIRQHADLSGAW"
     gene            complement(8136..8360)
                     /locus_tag="VFE39_00930"
     CDS             complement(8136..8360)
                     /locus_tag="VFE39_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257189.1"
                     /translation="MRDLFRPVSAPARQRGAANPASPFSGFGLMSDEVLTPGDGVRGV
                     RVVYRYRIAGGPTQGLVLRRHRRREPPRPR"
     gene            complement(8360..8617)
                     /locus_tag="VFE39_00935"
     CDS             complement(8360..8617)
                     /locus_tag="VFE39_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257190.1"
                     /translation="MTNSAARIYPKVPNWWRPIHARGADDAIGLDPSLIESQRGFWLA
                     GYDADARPSPAHMLGPHSPAPAAFIGVRTSRLWLVARSRST"
BASE COUNT         1164 a         3265 c         3098 g         1349 t
ORIGIN      
        1 ccgatctcga cctcgaggat gatcttcgcc ttgacggcct ggtcgagcag ctccgcggcg
       61 atccgcaggt tctcgtgcag ctcggtggcc gacccgtccc acatgtgcga ctggaacagc
      121 gggttctccc cgcggtcgac ccgctcctgg ctgatcgcga tcagcggccg gacgtagctg
      181 tcgagcttgt ccttcgggca gtggtcggtg tgcagcgcga cgttgatcgg gtacttcgca
      241 gcgacgacat gcgcgaactc ggcgagcccg acggcgccgg tcaccatgtc cttcaccttg
      301 gtgccggaca ggaactcggc cccgcccgtc gagatctgca cgatcccgtc actctccgcc
      361 tcggcgaagc cccgcaacgc agcattgagc gtctccgtgc tcgtcacgtt gatcgccggg
      421 tacgcatact cgccgctctt ggccttgtcc agcatctcgt tgtacttctc gggggtggcg
      481 atcggcacgg tctggccctc ctcgacgtcc gcggcttccg gcccgcagta tcccgcactc
      541 ggagggcgac gtccccgtca gcggacaagt gcccactact aggctgcgtc gcatggccga
      601 cgacggtggg gggaacgccg tcgagcagac cctgagcagg ttgcgccgca tcgacacacg
      661 caccgagcct gccccggctc cgggcgcccc gcgcacgctg tccgacctct accgggacca
      721 ccggatgcgc atggtgcgcc tcgccgtcct gctggtggac gacccgagca ccgcagagga
      781 cgtcgtgcag gaggcgttca ccggcctgca ccggcactgg tcggggctgc gcgacgaggc
      841 ggccgccatc ggctacctgc gcacggctgt ggtgaacggc tcgcggtcgg tgctgcggcg
      901 caggcgcacg gcgcgcgact acgtcccgcc gcaccaggtc accgcccgtt ccgcggagtc
      961 gctcgcgttg ctgtccgccg aacaccaggc cgtggtggac gcgctcgcca ccctgccgcc
     1021 gcggcagcgc gaggtgctcg tgctgcgcta ctacggcggg ctgtccgagg ccgagatcgc
     1081 cgacgcgacg ggcatcagcc gcggcaccgt gaagtcgacg gcgagccggg cactcgaggc
     1141 cgtcgcgcgc gtgatgagca caggtggggc acgatgacat ccgtgagcac cagcgacgat
     1201 tccgagcgac gggtcgccga agcactgcgg gccagggcgt cgggcgcggg gcgcgccggt
     1261 gctcgtcgca cggcgccgcc gatgccgccg gccgcacggg gcgtgcaaac ggccctgctg
     1321 atcggactgc tcggtggcat cgtgctggga atcgtgctcg cgctgctctc cctcctgacg
     1381 ccgggagtcc tacccgctct cgggtgaccc gggcaggccg gtaccgtcct tcctcgtgac
     1441 tgcgctcgcg ctcggccccg agtggctcca gcccgattcg atcatcgcgt ggctcggtcc
     1501 gtgggcactc gtcggactgg ccctgatcgt cttcgccgag tgcggcctgt tgctcggttt
     1561 cttcatgccg ggcgactccc tgctgttcac cgcaggcctg ttcgtcgcac agggggcgat
     1621 ccaggtcccg ttgtggctgg tgtgcgcgat tctcgtggtc gcagccttcg ccggtaacac
     1681 cgcgggttac tggatcgggc gcgcagccgg ccccgcggtg ttcgacaagc cgaagtcgcg
     1741 gctgttcaag ccgcagcacg tgcgcaagac gcaggacttc ttcgacaagt acggcaaccg
     1801 cgccatcgtg ctcggccggt tcgtgccggt cgtgcgcacc tttatcacgg tgatggcagg
     1861 tgtcggccgg atggagccga agcgctacct cacctactcg ttgatcggcg gcgtcgcctg
     1921 ggctgcaggc gtcaccgttc tcggcttctg gctcggccag ttcgtgttcg tgcgcgcgaa
     1981 catcgagctg atgctcatcc tcatcgtgct cgtctcggtg ctgccgatcc tcgtcgagct
     2041 ggtacgcgcc cgccggtcgg gcggagcaca cgccaagtaa caccgctccg gacccccgcg
     2101 aggccaggag tacctaacct ggagcctcgt gagtcccacg tccacgctgg atcacgctgt
     2161 cggtccgctc gccgaggccg gccccgtcac cgtgtggatc gtcgtcctcg cgttcgtctt
     2221 cctcgagtgt gcgttcatcg tcgggatgtt cctgcccggt gacacgctgc tgcttgctgc
     2281 aggcgtcgta ctcgcccaga gcgaacacga ggccggcgca tggaccctcg ggatggtggc
     2341 cgcggccgtc gccgtggccg gcaaccaggt cggttacctc gtcggccgct acgcgggcac
     2401 ccggatgctc gcccgccgcg agggccggat actcaatcgc gccaacctgg cacgggccgc
     2461 tgccttcttc gaccgctggg ggttctgggc gatcgtcgtg gcccgttggg tcccctgggt
     2521 gcgcacactc gcgccgatga tcgccggcgc cgcccgcatg gacaaccgcc gttacatcgt
     2581 cgccaacgcc gtcggcgcga tctgctgggt gcccacgctg atcatgctcg ggtactacgg
     2641 cgcggggctg ctcgacgtcg tgccgtggct caagaccgcg acgaccatcg ccgcggtcgc
     2701 cttcttcgtg gtcggcacgg gctacgggct ctaccgctac caccaggaga tgcgccgccc
     2761 ggtcgacgac tcgcctgcgg accctgtgcc tgccggggag gaccaggacc tgcgcgacgc
     2821 cggctgagcc accgggtcga gcgcgtcaac cggaacgctg cagcctcgcc gcggcctcca
     2881 gcaccatcca tcccgagagc tggaccgaca ggtcggcctc cggcacccct cgtcgtggtt
     2941 cgaaggcagg cgtgccccac tccgcggaga acacgggccg gccgcgaacc tccgcccggc
     3001 cgttccacac ggcgagagcg cccgcctgca cgatccgtgc cgccggtccg gccagctcgg
     3061 gcacgcgcag cgcggcatcg gcgaggtagc gggcggtgat gccggcgaac aggccaccgt
     3121 caccgccacc tgatccgggc agcactccgc gtggatcagc cagccggtgc gtcatcgcgt
     3181 cgacgatcgc gatggcgcgg tcggtgaagc gttggtgccc gtcccgctcg gccagctcca
     3241 cgcaggcacc gaggtggaca ccctggcagt aggtgtagat gtacggctcg accgcgcgga
     3301 tgctcccgtc ggacgccagc cggacgccgt cctggacgag cccggagtcg gggtccacca
     3361 gcgtctcggc catccagtcg gcaatggcgg cggcgaaggc cagctggccg tctcgggcca
     3421 gcaggatcgc ggccggtccg ttggccgggg cgttcttgaa gtcgtcgtcg cgccgccacc
     3481 agataccgcc gcctgccccc tccgtccagc cgtcgtccag ccggttcatg atcgcggcga
     3541 gcgccggcgg accggagcgg ccggcaaggg ggcccgcgcg ttgcaccgcg agcccgagcc
     3601 acgcgatgtc gtcgtagtag cggttgagcc acgagccggc gttgcgcagg tggacgccgc
     3661 ggaccagtgc cgtgatcgcg tgagcccggc gctgcgccgg cgaccgctgc tgggcgtcga
     3721 gcaggcagtc caggaggtgc gcctgccacc agtagtgcca cggcgccagg ggcgcgggca
     3781 gcggccgcgg ccaccgcacg cgcccgatgc gggtgcccgg cacgacccgg gcaacccgtc
     3841 gcaggtgccg gcgcacaacc gcccgctcgg cgatgtccgc tcgctccgac cagtcgacct
     3901 ccgcggcagc cacggatcca gtctgcccga tggccgaatg cgtgggtttc ctgtcgttga
     3961 cgccaacacc tgcgaccgac accgtgagta cgtgcccgaa cacgatgtcc tcatcccggt
     4021 cttcgacggc atccagccgc tcgacgccgc gggcccgcac gaggtgctga tgggtgctac
     4081 gagactgctc gcactgatcg gctcagagcc gggctaccgg gtgacgttcg tagcggccgc
     4141 tgccgcggga acggtcacgt ccgacagcgg gatgcggctg tgcgcggacg gtccgctgcc
     4201 ggagtcgggg cagatcggca ccctgctggt ccccggcgga tcaggtgcgc ggggcatgac
     4261 ccccgacgac ccgttcgtcg gctggcttcg gcgcgcggcc acccgctcgg cgcgggtggt
     4321 ctcggtgtgc accggcgcat tcccgctcgc cgcagctggg ctgctcgacg gcctgtcggc
     4381 caccacgcac tggcgctacg cgcacgcgct cgccaccgcc tacccggcag tcgacgtgca
     4441 gcccgacccg atatacctac ggcagggccg catctggacg gccgcaggcg tcaccgcggg
     4501 cgtggacctc gccctcgcac tcgtcgaggc cgaccacggc gtcgacgtcg cacagttcat
     4561 cgcgcgcgaa ctcgtggtgt tcctgcgcag gccgggcggg cagagccagt tcgccgggcc
     4621 ggtctggtcg ccgcccgcgc ggcagccctc gatccgggcc gcgcaggacc tgatccacgg
     4681 cgacccggct gccgacctgc gggtaccggt cctcgcggcg cgcgtcggca tgagcgagcg
     4741 ccacttcagc cgcgagttca cgcgcgcgat cggccggccc cctggcgact acgtcgagca
     4801 ggtccgcgtc gacgtcgcca aacgcctgct cgagtccgag ccggtgctcg tcaccgtggc
     4861 cgcggcgcgg tccggcttcg gcaccgccga gacgatgcgg cgggcgttcc tgcgccgcgt
     4921 cggcgtcgca cccgatcagt accggcggta tttcgccacc gcctcccaca ccgccgaaag
     4981 gacgtgaccg tgcaggtcgc catcgccctg ttcccccgcc tgaccgcgct cgacgcgatc
     5041 gggccctacg aggtgctgca acgggtgccc tcgctcgaca tcgtgttcgt cggcgagcgg
     5101 cgcggcgagg tccgcaccga gaacggcttc ctcgggctca ccgtggacgc gacgttcgac
     5161 gaggtgccgg cgccggacgt cgtcctcgtc ccgggcggga tcggctcccg cgagctgctc
     5221 gaaagcggga agttcctcga ctgggtgcgg tcggcccacg cgggcacccg cttcacgaca
     5281 tcggtgtgca gcggctcgct cgcactcgcg gccgccgggc tgctcgacgg gctcaccgcc
     5341 accacccact ggggtgccat ggagcacctg gggaagctcg gggcggtacc ggtgacacag
     5401 cgggtggtcg agcacctgcc cgagcgggtg atcaccgcgg cgggggtgtc ggccggcatc
     5461 gacatgggga tccggctcgt cgagctgctc gtcgacgcgg tagcggcgca ggcggcgcag
     5521 gtgtacatcg agtacgaccc acagccgccg ttcgatgccg ggcacccgag ccgggtgacc
     5581 accgaggtga tggaacgggt gcacgagcac cgacaggtgc gggcatgagc acaccggccc
     5641 ccaccgcacg cgccgtcctc agccactgca ggttctggcg ccacttcctc gagatgctcg
     5701 tcgcgatggt cgtcgggatg gtggcgctcg ggccgctgtg gccggaccca gggcggcacc
     5761 gagctccacg tcctcgtcat ggcgacgaac atgacgatcg gcataaccgt ctggatggcc
     5821 gtccgccggc accggcgggt ggacatcgcc gagatgggcc tggcgatgta gctgcccttc
     5881 gtggtgctgt tcccgccgtt ctgggccggc tggctgtccg caggcgcgct gtccaccctc
     5941 ggccacgtgc tgatgctgcc cgcgatggtc ctggccatgc tgcgcacgcg gccgagtaca
     6001 cctgctagcc cgtcagaagt gctccacgag ctccgggaat cgctgcaggt gcagcacgcc
     6061 gccatcgtcg gggtcgagct cgtccttctc gagcgcccag gtgtgctggt tcgggatgaa
     6121 caccgcaccc agcccggccg cgcgggcggg caggatgtcc gagcgcggcg agttcccgat
     6181 catccacgtc gacgacggag cgagcccctg ctccgcgacg agctcgcggt aggtcgtcac
     6241 ccgcttctcc gccacgatgt gcacgctgcg gaagtggtgc gcgaggctcg acgcgtcgat
     6301 cttgcgctgc tgctcggccg ggtctccctt ggtgagcagg aggaggtcgt ggcgggtgcc
     6361 gagatcggcg agtgtctcgg ccgcccctgg gacgagctcg acctcgtggt tcacgagcgc
     6421 cgcggcgaga tcggcgatcc ggcggacctc cgtcggcgtc gcggggcgct cgttgagccg
     6481 ctcgaaggcg tcaccgaggc tgcgcaggaa gctggcgctg ccgtacccgt ggacggcgac
     6541 attggcgcgc tccacctcgt ccaacaccgc gcgggtggcc gcccggtcga gcgtggggtg
     6601 cgcgagccag tcgaggtacg cgtcgatcac gcgctcgaac aggacgttgt tctcccacag
     6661 cgtgtcgtcg gcgtcgaaca cgagggtctg gcggctgggc acgcccggag tctaggaacc
     6721 ctggggcccg gtcctcgtcc tgttttccgg gccgggttcc gtcggtccca ccgcgtagct
     6781 tcccgccaca tggacacccc cgagcgcttc atccaccaga tcgaccagta cgacgcctgg
     6841 cagcgggaga ccatccggcg gtacggctgg gcgctgcagg ccgtgctcgg tgacggggag
     6901 gggccaccgt tcgtctacac ggtcgggctc tccggcttcg gtcaccccga gctcatcctg
     6961 ttcgccacct cgcaggccac cgcagccacg gtcctcaacg acctcggcga gctcgtccgc
     7021 gcaggccacg ggctcgcggc aggcgagctg atacgactgc gcagcggcga cgtgcacctc
     7081 ctggcgttcc ccgagtcggc ggcgtggctg ctcgcggcca acgacctgta ccgcatattc
     7141 gacgggcctc ccgtgcccgc gctgctggtc gttcccgccg acgagagggt gcccaccccc
     7201 ggcgacggcc agccctgcgt cttctgctcc tgagcgcggt ccgctcacca cgcgcccgac
     7261 aggtccgcgt gttggcggat ccacgcgtgc atcgcgatgc cggcggccac tcccgcgttg
     7321 atcgagcggg tgctcccgaa ctgtgcaatc gacactgtca tcgtcgcggc agcgtgtgcc
     7381 tcaccggtca ctcccgggcc ttcctggccg aacagcagca cgcaatggcg tggcaggtcc
     7441 gcggtctcca ggcgctgcgc gcccgacgtg ttgtccaccg ccaccacagc cagcccatgg
     7501 cccgccgcga acgccacgag gccgctcagc gtctcgtggt ggtgcacctg gaggtaacgg
     7561 tcggtcacca ttgcgccgcg ccggttccat cgccgccgcc cgacgacgtg gacgccggcc
     7621 gcggcgaacg cgttggcggt gcgcacgacc gtgccgatgt tgtggtcgtg tccgaagttc
     7681 tcgatcgcca catggaaggg gtgcgcccgc cgagcgaggt cggcggccac ggcgtcacgg
     7741 cgccagtagc ggtaggcgtc cgccacgttg cgcgcgtcgc cgtgctcgag cagttcgggg
     7801 tcgtactgct catcccgggg ccacgcgtcc cgaccgcccg gccacggccc cacccccacc
     7861 tgaccgggca cggcccactc actcggcccc gccccctccc cctccccacc ctcgctgccg
     7921 ttccgcgagt cgcccgctcc gggtgcgcga gtcgcccagt ccggctggtc gagtcgcccg
     7981 ccccgggtgg acgcgccctt cgctgctggc ccgttcccgg ccgctgcagt cgggtcctcg
     8041 ctcatgaccg catggtctcg cgccggcccg ctccgctcga gcccgggagc cctcctcgtc
     8101 ccggaggtgc gactcgcgca cgcagggcgc cggaactacc ggggccgtgg gggctcgcgg
     8161 cggcggtgtc ggcggagtac gagtccctgg gtcgggccgc ccgcgatgcg gtatcggtag
     8221 acgacccgca cgccgcgcac gccgtcgccg ggcgtcagaa cctcgtcgct catcaacccg
     8281 aacccgctga agggggacgc ggggttggcc gccccgcgtt gcctggccgg agccgaaacc
     8341 ggccggaaca ggtcgcgcat caggtcgagc gagacctggc cacgagccac aggcgggatg
     8401 tgcgcacacc gatgaaggcc gcaggcgccg gcgagtgcgg cccgagcatg tgcgcgggcg
     8461 acggtcgggc gtcggcgtcg tacccggcga gccagaaccc acgctggctc tcgatcagcg
     8521 acgggtcgag cccgatggca tcgtcagcgc ccctggcgtg gatgggccgc caccagttgg
     8581 gcaccttcgg gtagatgcgg gcggccgagt tggtcacgca ggtgaactca gggcccgcgc
     8641 acgcgcacgg cagtgcagcc gcggcggaca gaagcacacc actgccgtct gacgacggtc
     8701 ccgaccagcc cggaccgtca tccgacctcc taacgcgatc agtgattaca ccgtgatatg
     8761 tgtacggggc acacgcaagc gcggcggatg ggctgcatca gtgcgaccat gtgtcggaac
     8821 gcacgcctga ccggctgacg ccccgcccgt cgcgccggcg gccccgtccg cccggg
//
LOCUS       DASRSC010000023         4318 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4919797,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000023 DASRSC010000000
VERSION     DASRSC010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4318
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4919797"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="VFE39_00940"
     CDS             <1..570
                     /locus_tag="VFE39_00940"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6257191.1"
                     /translation="QIERDLHDGAQQRLVTLGLELSLLQSAAHPDLDELRSGIGRVSA
                     ELTEVLDELREMSRGIHPAILSEGGLCPALRALARRSAILVELDLQTQARFPGPVEVA
                     AYYVVSEALTNMTKHADASYVTVVMEERYGSMRLSVQDDGVGGADPARGSGLVGIRDR
                     VEALGGTFEVSSPVGEGTLIQVALPLETG"
     gene            complement(580..1224)
                     /locus_tag="VFE39_00945"
     CDS             complement(580..1224)
                     /locus_tag="VFE39_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6257192.1"
                     /translation="MRVVIAEDDVLLREGLASLLERTGFQVVGQAGDAARLLELVRET
                     APELVVVDIRMPPTQTNEGLEAAREIRREQPGTGILVLSAHIEVEHAMELLASGQGVG
                     YLLKSRVTEVAEFIDTLERIARGGSVVDPALVQELVAARKRNDPLAVVSARERDVLTL
                     MAEGRSNAGIARRLWISEGTVEKHVRSILTKLSLPETDDSHRRVLAVLTFLESR"
     gene            1655..2596
                     /locus_tag="VFE39_00950"
     CDS             1655..2596
                     /locus_tag="VFE39_00950"
                     /inference="COORDINATES: protein motif:HMM:NF020919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HET6257193.1"
                     /translation="MLSLTLMLAGCGTPGTSEHSGHSGVAPVNYIDIGQVAVVPPAPV
                     VRPDGSTGTWRINCGRNERGLYNSDNLVASPGVAGGAHHFHDYVGNVSTNAFSTDQSL
                     AAADTTCPDDDRSTYYWPVLREPGPGGAAQHDNSADGNMGRILVPASVLLEFRGSPVS
                     SVVEMPRFMRATTGNPHGFSTGGVNTEHVQWTCSGARDRITKEYPRCAPGQQVVRIFD
                     MPSCWNGTTTDSADHRTHLVFPDSSGACRSGTFPVPQLHMEIAYSVTPDSDYTIDSFP
                     EEQHSPVSDHADFVNVMPDSLMDTVVSCINSGQHCGS"
     gene            3139..3687
                     /locus_tag="VFE39_00955"
     CDS             3139..3687
                     /locus_tag="VFE39_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6257194.1"
                     /translation="MAGPKARDRRNMADEALVRSLFNEHGKAMLGYATRLTNDRAAAE
                     DVVQEALLRAWRSPDSLVNSKGSVRGWLLTVVRNIIIDRARARNARPTEVAESPSTVP
                     VESDHADRVVDTMVVLDALEHLSSEHREVLEQMYLQGRTVGETAEVLGIPTGTVKSRS
                     YYALRALRDFFPKRPTGMEGAA"
     gene            3687..>4318
                     /locus_tag="VFE39_00960"
     CDS             3687..>4318
                     /locus_tag="VFE39_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6257195.1"
                     /translation="MRTSDEHVDIDGYIVGDLDADRARAVEAHLDECPECREEVHTLR
                     ELQEFLVTVPPEALLDGPPDDADLLLQRTLRQVRTERSGTSGRRTRWAAAASAVVAAA
                     AVCVGVVVGRGTVDTQQVIPAPHASSPSASSPEVPGTRFASAVNPSTGARLTVRVVPA
                     AGWVRINAAVTGIPAGQRCHLVVVGRDGHRETAGSWLVSETAAHQGVNLEG"
BASE COUNT          684 a         1431 c         1529 g          674 t
ORIGIN      
        1 cagatcgagc gggatctgca cgacggtgcg cagcagcggc tcgtcacctt ggggctcgaa
       61 ctgagcctct tgcagagcgc ggcgcatccg gacctggacg agcttcgttc cgggatcggc
      121 cgggtctccg ctgagctcac cgaggtgctc gacgagctgc gcgagatgtc gcgcgggatc
      181 caccccgcga tcctctccga gggagggctc tgccccgccc tgcgggcgct cgcccggcgt
      241 tcggcgatcc tggtcgagct cgatctccag acgcaggccc gcttccccgg gccggtcgag
      301 gtggcggcgt actacgtcgt ctccgaggcg ctgacgaaca tgacgaagca tgctgacgcc
      361 tcgtacgtga cggtcgtcat ggaggagcgg tacgggtcca tgcggctctc ggtgcaggac
      421 gacggcgtcg gcggtgccga tcccgcacgc ggctcgggac tggtcggcat ccgggaccgg
      481 gtggaggccc tcggcgggac gttcgaggtc agcagccccg tgggtgaggg cacgctgatc
      541 caggtcgccc ttccgctcga gacgggatga ccgtcgcact caccgcgatt ccaggaacgt
      601 cagcaccgcg agcacgcggc ggtggctgtc gtcggtctcg gggaggctca gtttggtgag
      661 gatgctgcgc acgtgcttct cgacagtccc ctcactgatc cacaggcggc gggcgattcc
      721 ggcattggag cggccctccg ccatcagcgt caggacgtcc cgttcgcggg cgctgaccac
      781 ggcgagcggg tcgttgcgct tgcgggcggc caccagctcc tgcacgagag cggggtcgac
      841 gacagaaccc ccgcgggcga tccgttcgag tgtgtcgatg aactcggcca cctcggtgac
      901 gcggctcttg agcaggtacc cgaccccttg gccgctggcc agcagctcca tggcgtgctc
      961 gacctcgatg tgagccgaca gcacgaggat gcccgtaccg ggctgctccc ggcggatctc
     1021 cctggccgcc tccagcccct cgttggtctg ggtcggcggc atccggatgt cgaccaccac
     1081 cagttcgggt gccgtctcgc ggacgagctc gaggagacgc gcggcatcgc ctgcctgccc
     1141 gacgacctgg aagcccgtgc gttcgagcag gctcgcgagg ccttcgcgga gcaggacgtc
     1201 gtcctcggcg atgaccaccc gcagcggcgt catggccgca cccgcgccag ccctgcgcgg
     1261 gcgagggcga gcgggtacgg cgcagtactc atggaaggga ttatgtcgac gtttcgagcc
     1321 cgtagcgacc cgacggcagc actggccccg ttcggacgcc tcctgatgcg actgcccgga
     1381 cgtaccgtgc tcgtccgcgc cgaccaccac ccggtcgcgg acggaaccgg ggacgccggc
     1441 cggcgtgcgc acctcgtcac catcggcgtc acgagcggcc gacgcatttc ttgacgtcgc
     1501 ccctgcgatg cagtccggcg ccgcggtaga gatctgcaca tccaggcgaa atcgcataat
     1561 ccgacgcgcg cccgcgagat gcctctgcga caatgcgacg gtgcacggga tcggggttcg
     1621 gtgctcgttg gccgggtgga ttgtcagggt cgcggtgctg tctctgaccc tgatgttggc
     1681 cggctgcggt acgccgggaa catcggaaca ttcgggacat tccggagttg cgcccgtaaa
     1741 ttatatcgac atcggtcagg tcgccgtcgt gccgcccgcg cccgtagtgc ggccggacgg
     1801 ttcgaccggc acctggcgca tcaactgcgg acgcaatgaa cgaggacttt acaattcgga
     1861 caacctggtc gcaagtccag gtgtcgccgg cggcgcgcac cacttccacg attatgtggg
     1921 caatgtctcc accaacgcct tctccacgga tcagagcctg gccgcagccg acacgacgtg
     1981 tcccgatgac gatcggtcca cgtactactg gccggtgttg cgggagcccg gaccgggcgg
     2041 cgccgcccag cacgacaaca gtgccgacgg caacatgggc cggatcctcg tgccggcttc
     2101 ggtgctgctc gagttccgcg gtagtccggt gagttccgtg gtggagatgc cgcgattcat
     2161 gcgggccacc actggaaacc cgcacgggtt ctccaccggc ggggtcaaca ccgagcacgt
     2221 gcaatggacg tgctcgggag cccgggaccg catcaccaaa gagtatccgc ggtgtgcgcc
     2281 gggtcagcag gtggtcagga ttttcgacat gccgagctgc tggaacggca cgaccaccga
     2341 cagtgcagat catcgaacgc atctcgtgtt cccggattcc agcggagcct gccggagcgg
     2401 cacgttcccg gtgccgcagc tacacatgga gatcgcctat tcggtcacgc cggactcgga
     2461 ctacactatc gacagctttc ccgaggaaca gcacagccct gtttccgatc acgctgactt
     2521 cgtcaatgtg atgccggact cgttgatgga tacggtggtc tcctgcatca actcgggaca
     2581 gcactgcggg tcgtgaggct cgggatggcc cgctcaggcg ggccggcgtc ctgggctcct
     2641 gcggctcgac gccgagcggc ggatcgaact cttccgaaaa tgggtgaacc ggtccgctgt
     2701 ccgcagccgt gtcccaggcg atgcggtcga cactccgccc gctcgacggt ggcgaagggc
     2761 gaaaacgatg gaggcggcca cgtgcagcga cggacgggcg ggatgtgctc ggtcgacgag
     2821 cgtggacagc ggccaccggc cgacgtctgt tcagctcgcc ggccacgtga accagcagct
     2881 gacggtcgga ggccgtcccg cctcccggtt ccagaacgac caggtgtccg gtcggaacga
     2941 gggccagggc gtcggcggga tgtggatcgt cctcggtcca ggtggctgaa gatcatgcac
     3001 cggaacaccg gggtcgtgaa tgcccgatcg gccgacaggc attgctctat cgcggtggtg
     3061 gcaatccgag ctgccatcgc cgcgcgtcct ccagacacga ctcgagtggt ccgcatcgcg
     3121 gtggaaggtg gtagggggat ggccgggccg aaagctcgcg accggcggaa catggcggac
     3181 gaggcgctcg tccgctcact gttcaacgaa cacggcaagg cgatgctcgg gtacgccacc
     3241 cggctgacca acgaccgcgc cgcagcggag gacgtcgtcc aggaagcact gctcagggca
     3301 tggcgcagcc cggacagcct cgtcaacagc aagggttcgg tgcgcggctg gctcctcacc
     3361 gtcgtccgca acatcatcat cgaccgcgcg cgggcccgga acgccaggcc gacggaggtg
     3421 gccgagtctc cgagcaccgt cccggtggag agcgaccacg ccgaccgcgt ggtcgacacg
     3481 atggtggtgc tggacgcgct ggagcacctg tcgagcgagc accgcgaggt actcgagcag
     3541 atgtatctgc agggacgcac ggtcggggag acggccgagg ttctcggcat cccgaccggc
     3601 acggtgaagt cgcggtccta ctacgcgctg cgggctcttc gtgatttctt ccccaagcgc
     3661 ccgaccggaa tggaaggtgc cgcgtgatga ggacgtcgga cgagcacgtg gacatcgacg
     3721 gctacatcgt cggcgacctg gacgcggaca gggcccgggc ggtcgaggca cacctcgacg
     3781 agtgccccga gtgccgggag gaggtgcaca cgctccgcga gctccaggag tttctcgtga
     3841 cggtcccgcc agaggcattg ctcgacggcc ctccggatga tgcggatctc ctgttgcagc
     3901 ggacgctgcg ccaggtgcgc accgaacggt ccgggacaag tggccgccgc acgaggtggg
     3961 ccgccgcggc ttcagcggtc gtggccgccg ccgcggtctg cgtgggcgtc gtcgtcggcc
     4021 ggggcacggt cgacacccag caggtgatcc ccgcgcccca tgcgtcgtca ccgtcggcga
     4081 gctccccgga ggtgcccgga acacggttcg cgagtgcagt gaacccgagc accggggcgc
     4141 ggttgacggt gcgtgtggtg ccggcggccg gctgggtgcg gatcaacgct gcggtgaccg
     4201 gaatcccggc aggtcagcgg tgccacctgg tcgtggtggg tcgggacgga caccgtgaga
     4261 cggcgggaag ctggctcgtc tccgagacgg ccgcgcacca gggcgtcaac ctggaggg
//
LOCUS       DASRSC010000024        27377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3609409,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000024 DASRSC010000000
VERSION     DASRSC010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 27377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27377
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3609409"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..580
                     /locus_tag="VFE39_00965"
     CDS             248..580
                     /locus_tag="VFE39_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HET6257196.1"
                     /translation="MSTTGERSTGTVYEQVQASPEFADLRSRLRRFVFPMSVAFLAWY
                     LAYVLLASYARGFMAIKVFGNINVGLIIGLLQFVSTFAITTIYVRYADKHLDPAAEKI
                     RQQIESAE"
     gene            577..2217
                     /locus_tag="VFE39_00970"
     CDS             577..2217
                     /locus_tag="VFE39_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HET6257197.1"
                     /translation="MSMVAQAPAPPAATAVGSVPLNIGIFVAFVAVTLVFVYRASRTN
                     KTASDYYAAGRAFTGPQNGVAIAGDYLSAASFLGIAGAIALNGYDGFLYSIGFLVAWL
                     VALLLVAELLRNTGKFTMGDVLAFRMRQRPVRAAAATSTLVVSFFYLLAQMAGAGGLI
                     ALLLQIPTSNRVGQGAVIAVVGILMIVYVLVGGMKGTTYVQIIKAVLLIIGAAVMTVW
                     VLGIVGFNLSALLGDAAAASPKGEALLAPGLQYTNPIDLVSLGLALVLGTAGLPHILM
                     RFYTVPTAKEARRSVVWAIWLIGLFYLFTLVLGYGAAALLPPGTITSATQNNAAPLLA
                     YEVGGELLLGIISAVAFATILAVVAGLTITASASFAHDVYANVLKRGAATPDSEVRVA
                     RITALVIGVVAIVGGIIANGQNIAFLVALAFAVAASANLPTILYSLFWKRFNTTGALF
                     SIYGGLAICIILIVFSPAVSGKPVNPATGRSASMLQGINFHWFPLDNPGLVSIPASFL
                     LGIIGTYVGGRRPIEEGKFAEMEVRALTGAGSEKAIAH"
     gene            complement(2276..2644)
                     /locus_tag="VFE39_00975"
     CDS             complement(2276..2644)
                     /locus_tag="VFE39_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257198.1"
                     /translation="MDYAGVTGQWAVTVARRLHCRGIETPPPSIDETWVGTDLALYLR
                     YRVGAHKLAARFRLDVDPSSGAWPTDADQFATAVFHALPAEPEPDPFVDERGYRWWSA
                     AVPHIGLAAAVQSRRIVTIS"
     gene            2740..3072
                     /locus_tag="VFE39_00980"
     CDS             2740..3072
                     /locus_tag="VFE39_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6257199.1"
                     /translation="MTGPAVPAVPVADLPPDAALLDVREIDEWNAGHAPGARHLPMSE
                     LTARIAELPDHDPLYVVCRSGGRSARVVAYLAGQGYPVVNVEGGMQSWAAQGRELVAD
                     GGSGPEII"
     gene            3078..4046
                     /locus_tag="VFE39_00985"
     CDS             3078..4046
                     /locus_tag="VFE39_00985"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HET6257200.1"
                     /translation="MLPTAPSPGAARLGPPCPRCGRRAPAGGGPFCPYCGRYLAALRW
                     VAEPPPSTTKPAPARVRTRYTGPPRYRSLPRWGFPPRGWEPPDPASPPRSAPLDAARS
                     LSGSAVPLLWATAAVALLAAFAEVWRYVLLLASRDGALSADAVAASDALVASAGTIAP
                     ILTLGTGGLLVLWSVRASQAAADRAGVRPSRSARAIVLGWLVPGLNLTVPGSVLSEIE
                     HSALGLPADRRPRPSRLLLIWWGLWATGVVLAAVVLLWSLRDGVQARADGVVLHAVLD
                     LLAAATAGVTARLVTWHMRLLGPPRPRRRELVRSITPPAAARAARA"
     gene            complement(4009..5181)
                     /locus_tag="VFE39_00990"
     CDS             complement(4009..5181)
                     /locus_tag="VFE39_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6257201.1"
                     /translation="MNPFDPGDPRFLADPYPIFATLRALGPVHAHPLLGTPVAVSHAA
                     CSELLRGRELGRIWVDAEPAADFPAFNLLHRNSLLEREGPAHARLRGLVAGAFNRGHT
                     ARLEPWVRGLAHRLVGELAERIDADGAADLMSGVAARLPVEVIAELLGVPEPERSALR
                     GWSNAVVTMYEPDPGQERRRAAEQAAADFVDVLRGLVAHRRTHPGDDLVTHLVEADVD
                     ADELVGTAALLLMAGHEATVNVIGNGVLALLRHPTQWQRLVADPGLDATAVEELIRYD
                     PPLQLFERTAVVDTTVAGFPVSAGTRIAALLGAAARDPQAFDRPDELDVGRRRNPHLG
                     FGAGVHYCLGAPLARLEVVAALDALRTRLPGMRLADEPHRRPDFVMRGLRELRLAA"
     gene            5286..6260
                     /locus_tag="VFE39_00995"
     CDS             5286..6260
                     /locus_tag="VFE39_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5926 family protein"
                     /protein_id="HET6257202.1"
                     /translation="MAKKTRARREGTTKDSASAATSAGENPRRPCPCGSGKRYKACHG
                     AGDDVIVLRPFEGLAAECDLVAMREFLPSATAPLPLREPLDVSVTLATVLPGASAALV
                     RPDGTIMLGMQVQTRSGDLSADLAKALRWAQAAESGTALPVVGPGIGDGELVRLQDVL
                     DPAATLDVTLHADFGWWVVPAEDGAEPSADVTAGLERANAVIMPTEPVLAEGVRAAYW
                     VDTGTKAHLRWVRPEPEEKLLDALARLHARGELGLAEDARFAGAFRAHGLLVPVWDLD
                     RELHPKEWASPVATFATRLDAALADETPLTDAERRARDALAGKQVTLR"
     gene            6320..7237
                     /locus_tag="VFE39_01000"
     CDS             6320..7237
                     /locus_tag="VFE39_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6257203.1"
                     /translation="MGLLDGNVIVVTGAGRGIGRGEALECAAQGAAVVVGEFDPDAGK
                     AVADEITAAGGRAVAVIGDVADAAVADSLVATAVGEFGALDALVNNAGILRDRTVAKL
                     SDEEWDDVIRVHLRGHFLPTRAAVRYWKEAKRPGHVVHTASTSGLLGNFGQANYGAAK
                     AGIAAFSTIVAMEGARGGITSNAIAPTALTRMTLDLMPEEFRAEREASIARGEFDFFA
                     PENVAPLVAFLCSDASSHISGKVFGVQGDSVEIFQPFTSVAEIRNDGKRWDPEDLPGR
                     IDELFDAAGVQAGPENMMERMRYQILAGR"
     gene            complement(7243..8028)
                     /locus_tag="VFE39_01005"
     CDS             complement(7243..8028)
                     /locus_tag="VFE39_01005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HET6257204.1"
                     /translation="MSPPSPVAAPVTDGRTRRLVGTEVLVVLTVTLGLSALRSALSLL
                     DALLQTVPLNKQQVALNAPEAQAALVDLALQLTRVLQLVGWGALGAYLLIRAGIALRD
                     VGLDGRRPGRDALGAVGLAALIGVPGLGLYLVARAIGVSLTIAPSTLGDTWWRLPVLI
                     LSAAANSWAEEVVMIGYLITRLRQLGWSENRSLVAQALLRGSYHLYQGLGGFIGNLVM
                     GLVFGRVWQRTNRLWMLVGAHALIDVVAFTGYAVLAGKVGWLP"
     gene            8100..8576
                     /locus_tag="VFE39_01010"
     CDS             8100..8576
                     /locus_tag="VFE39_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257205.1"
                     /translation="MRQLVFSPRWMLWHVLTLGAMATCGWLAAWQWQRAGSAMGSALN
                     VGYGLQWPVFAVFFGVMWWRMLRMEAAKLQEGEADVAGEAPAPAGGGSAPVVAGQARS
                     AGPRSAEPRPRAASDAPSPFGPRPASVLPRVAAEDDPELAEYNQMLAHLAARDSTD"
     gene            8589..8906
                     /locus_tag="VFE39_01015"
     CDS             8589..8906
                     /locus_tag="VFE39_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3817 domain-containing protein"
                     /protein_id="HET6257206.1"
                     /translation="MSIETALRNYRVIAWVTGVGLLVLVLVAMPLKYFFGQPQLVALV
                     GMTHGFLYMIYIVCTLLLAERCRWKPLDALLILVAGTVPFASFVAERRVTHRVRAGRS
                     PVS"
     gene            complement(8903..9727)
                     /locus_tag="VFE39_01020"
     CDS             complement(8903..9727)
                     /locus_tag="VFE39_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2470 domain-containing protein"
                     /protein_id="HET6257207.1"
                     /translation="MTSLESTRLRRPPAPTDAERARSVVARGGTALLVGTGAPRTAPL
                     VHHVRADASAVLLLPDDDPVLDRIREAPDGTSSAMLELADHAPVDLRQPVRALLWITG
                     TLRLPHLEEARRLAVHVADVRPHPDLLQLGHGATLVRLDPGSAVLSDAEGSAALAPIE
                     LAAARPDPFCRYEQRWLAHLEEAHPEVLAALARHLPPPLLGLRGTRMRPLGADRCGLR
                     LRIEVPDGVENPGRDHDVRLAWQDTPTTVDELRVQMGLLVGCAGQRHGRGSARRNR"
     gene            9838..10722
                     /locus_tag="VFE39_01025"
     CDS             9838..10722
                     /locus_tag="VFE39_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257208.1"
                     /translation="MTTAAGVNSSWAIPPLFAHLVDDTSLLQPRSVAVGIDKVVSRYL
                     SARDGRYGNLVGQLVCPVSRLPALVAELARAVPSRPVELSLVVDTGLGGVPKALSTVF
                     SRSSLLTAHTVETAAPPDVDAVWLERVAEFVPEDVVAVVEPRRPSVAEPVGTGAWLDA
                     VRRVAEHGCSPKLRCGGPRASDVPSVTDIEEFVRVVVGAGRGFTTLGLRQVVHTDDGT
                     VPSQHGLLNLLVAVARALAGGDVRGALETTDGAALAVEIGGLSEQAVTGVRGLLSRCG
                     ADPEPVPAAQLAGLGLLA"
     gene            complement(10738..11253)
                     /locus_tag="VFE39_01030"
     CDS             complement(10738..11253)
                     /locus_tag="VFE39_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HET6257209.1"
                     /translation="MAAAWRTLAGTRPGLVVHEGSITDVDADAVVSPANSLGVMGGGI
                     DGVYARWLPRISDRVRAASGADRGGELPVGDAVIVPTGVARPGWLISAPTMRRPGELL
                     PADGGAARAAASAVLRLWRDGALPGNGTPVREAIRTIALPGLGTGVGGLDPDVCAHQV
                     GAALDEVLGPA"
     gene            11320..12267
                     /gene="pheA"
                     /locus_tag="VFE39_01035"
     CDS             11320..12267
                     /gene="pheA"
                     /locus_tag="VFE39_01035"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET6257210.1"
                     /translation="MPRLAYLGPRATFTEQALRSLPESSGAELVPCAGSPAVLAAVRD
                     GSVDAGCVPIENTVEGAVPAVLDGLVADPPLVIVREALVAVRFAVLVRPGTRAAGIRT
                     IASHTHAIAQTRGWIAAQLPDAEVLLSTSTAEAAAQVARAEFDAAIAAPLAAEQHGLE
                     VLVDDVADNPGAVTRFALLAPPGPPPAPTGQDRTTLAATTQNRPGALLGLLTELAVRG
                     IDLTRIESRPIKDRHAEYWFHLDCSGHVAEPAMGEALAALRRRCDRVRFLGSYPRAGN
                     GPQQTCGSDAPPVPVGGAGAAEFAAAQEWLAGLRAGSAT"
     gene            12264..12911
                     /locus_tag="VFE39_01040"
     CDS             12264..12911
                     /locus_tag="VFE39_01040"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6257211.1"
                     /translation="MTPGPLRLVLVRHGQTDANVRRELDSVPPGAPLNELGLTQAAAL
                     GERLSTFPVTRVHASRATRAQQTAAPVAAAHGVDVAVLDGVHEVFVGDLEGRSDRPAR
                     EQFNVVYGAWWNGDLDARLPGGESAKDLRARYLPVVKQITDGAHGDVVLVSHGAAIRL
                     AAAALLGDTAETLYVPNTGLVVLAADPSAPSGWALEHWDSAVPVPGDVTAGGTAG"
     gene            complement(12926..13264)
                     /locus_tag="VFE39_01045"
     CDS             complement(12926..13264)
                     /locus_tag="VFE39_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET6257212.1"
                     /translation="MGRRRFEEAVADALDEIPPQLTAAMDNVVVLVADRNAEEPDLLG
                     LYVGVALTERTTSYGGVLPDRITIYKDAILDVCDDVDEVVREVAITVVHEVAHHFGIN
                     EETLRELGWG"
     gene            complement(13281..14384)
                     /locus_tag="VFE39_01050"
     CDS             complement(13281..14384)
                     /locus_tag="VFE39_01050"
                     /inference="COORDINATES: protein motif:HMM:NF025215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation family protein"
                     /protein_id="HET6257213.1"
                     /translation="MATLDTFAGATVPVLGSLAALPGIAGEPDVVEAPAPPATPGTCL
                     NWTRPDAADTKVVDCAQPHLFEQAASVTLTDQTALPDDRQWRQLVNERCNQPVLDYLG
                     GKFDPDGRYRVGALKPSPTKWAEGDRELRCGLQSASRSGALYPLAGRVADSDQSAVHD
                     PGTCLAIDGRTIGDPVDCAGPHAVETVGIVDLSGKFPGAFPAVGDQDPFLQQECGKIA
                     NDYAGGADVIAAKKLTVYWDNLTEDSWKAGTRKVNCNLAALLPDRSGFAPVTGPVRGA
                     VVVGDTAAPPAPHTTGAGVPAPTTTTTPSPEPTDGPPAEQIPGPVDEPPAGEQPEPSG
                     GVVPPGPPARPSINPPTPPSFTPPAPPTPPAGA"
     gene            14869..15492
                     /locus_tag="VFE39_01055"
     CDS             14869..15492
                     /locus_tag="VFE39_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257214.1"
                     /translation="MPTDDDRPGGDEPGDRREPPTGRMRFQDESTTPREPTLAERRAR
                     EQAARREEEAERQQAEDQERSRKKRKRILIGAGATVGVVALIAVGYSVAQPDNEVQAQ
                     CVDEQTKVVVDDANCVTPASSGGSYYGGAGIVPYPIFLGGGGRQYRYNYGGSGNIGQP
                     VSGGTSVVPKAGTRVTTPSGKSVAGTSGSGSVSRGGLGVSNSGSSGS"
     gene            15515..16681
                     /locus_tag="VFE39_01060"
     CDS             15515..16681
                     /locus_tag="VFE39_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HET6257215.1"
                     /translation="MERRRGEPRPDWETTVRDQGMVYGTPGRGEGGAPRPYWDESVHY
                     VFSMDEILSLEADVEVVHSMCLEAVDHVVTTERFRDFALPEWSWEGVAASWRRSDPHV
                     YGRFDLRYDGGGPAKLLEYNADTPTTLLEASILQWNWLKDTHEGDDQWNSLHEKLVER
                     WELIKARLPGDEVHFTWSSGDSTGEDHVTVGYLQETAAEAGLNTVGLAIEDIGWDAAL
                     DRFVDLEEAPMAAVFKLYPWEWVLTDQFGKHVLRSLPETLWVEPLWKTLLSNKALLAV
                     LWEMYPGHPNLLPAYLDQPGLLTEYVRKPRLGREGANIAIVAPGMEHQTGGVYGEEGY
                     VYQLFDPLPEFDGYRPALGAWIVGDTAAGLGIRETVGLVTDDGAAFVPHRIPEP"
     gene            16681..17151
                     /locus_tag="VFE39_01065"
     CDS             16681..17151
                     /locus_tag="VFE39_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HET6257216.1"
                     /translation="MLPLEVSVTLAQIDPGFFSVIGRGIGAILLYAVLGVLLMLLGFY
                     AVDLTTPGKLNRMVRDGLPNAVLVTAAGMVSMAFIVVTAIYSSSGALLEGLLASLIFG
                     LVGIIAQVGGVRLLEWVTGIDIGAVLAAERVQPQAYVVSAAHLALGLVVAVAIL"
     gene            17244..18266
                     /locus_tag="VFE39_01070"
     CDS             17244..18266
                     /locus_tag="VFE39_01070"
                     /inference="COORDINATES: protein motif:HMM:NF014786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6257217.1"
                     /translation="MESLRGIGRMSRESGLAVSALRFYDGAGVLVPAHVDARTGYRSY
                     APDQVLAARLVAVLRRVGMPIAGIRGVLACRHDPAAVDELLAAHLHRLEQGLVDARRE
                     LSSVRSLIEQEEHPVSQTTVTVRGHELSDALRAVRFAVGADPELPVLAGVLLDVTAEA
                     VTLVATDRYRLAVSSAPVLTLDGPPVRAVVPAGLADRIPDAGVVSIAVDGTTITVLSD
                     DDVLSGALILDDFPDYRRLLGDVGRPVPVGRALRAALAAGPVRTMRREQDGAEFAVAV
                     LGPTPNGGITVDPVGDGVGVNREFLLEALDAGGEGQLLLSLDGPIAPLVIRSSRSVSL
                     LMPVRL"
     gene            18387..19196
                     /locus_tag="VFE39_01075"
     CDS             18387..19196
                     /locus_tag="VFE39_01075"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005460928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET6257218.1"
                     /translation="MQAPRLVATDVDGTLLDPDDRVSRRAAAVIGRAVAAGVHFVLVT
                     GRPPRWIPPVVDQLGVARIAVCANGSVLYDALEDRVLSSHTLDPATLAELAEAAAVAL
                     PGCGLAVERVGAGAFDDSAPFVAEPNYLHAWPDADNAVAVRSELLSRPAVKLLVRCPQ
                     LSSDAMVAALAPVVGDAADLTFSHPRGLVEISPHNVTKATGLAGVVHGHGLTAADVIA
                     FGDMPNDLEMLRWAGHGVAMGNAHPALLDVADEVTASNGDDGLALVLERWF"
     gene            19532..19981
                     /locus_tag="VFE39_01080"
     CDS             19532..19981
                     /locus_tag="VFE39_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET6257219.1"
                     /translation="MSLFGEHAAGWLLFGALGAMIDRRRRRDWVAATAAVAVAHGASV
                     GVKRVVRRPRPDNPRVQVLVGTPSKLSFPSAHATSTTAAAVVFGALLRKPLAAALVPP
                     MALSRMVLGVHYPTDVLAGSVLGAAVAVAARRLARRPTALRAQEGNE"
     gene            19978..20898
                     /locus_tag="VFE39_01085"
     CDS             19978..20898
                     /locus_tag="VFE39_01085"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HET6257220.1"
                     /translation="MTSTDVPTNQAASPRTPVSVTRGVLKTMRPRQWVKNVLVLAAPF
                     VAGDLFTAGIAIHLAVAFVAFSLAASGIYLVNDANDVAADRAHPTKRNRPIAAGIVAP
                     RLALSVAVVLLLGALALSLLASVQLVIVLGVYIAVQLAYCFWLKHQPVLDICVVASGF
                     LLRAIAGGAATGIALSQWFLLVAGFGSLFMVAGKRYAEMMLAERTGAKIRKSLESYSA
                     SYLRFVWALSATVLITTYCLWAFEIRAERHNTVWSVISIVPFVIAVLRYSVDVDSGNG
                     GEPEELALADHVLQVLALLWIGTLVLAVYA"
     gene            20984..22513
                     /locus_tag="VFE39_01090"
     CDS             20984..22513
                     /locus_tag="VFE39_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257221.1"
                     /translation="MTSARVIDPSSVRVAGRRVATVVPDLVMLVGVALAASAAFLLVH
                     RALIDDAYITMTYARNLAFHLHWGLITDETANSATSPLNVILLGAITFVLRNAQIAVG
                     VLFVASVVLATRWLTTLTTLLGLSRATPYLGVALLLLNPLLLSTVGLESYLAATLVIG
                     LLRFGAEGRSIAFGVVSGLAFLTRPDLAVVVLIAAVGFPGIRRRVVRAFAVAVLVAGP
                     WHLWAWFALGSALPDTLIMKAGSPTWSGFGFWDGALMYWGGAPIVTALAFLPVLLGAA
                     ALLAVLVARLFGHRDAAHRTAAVAGVGAGAHYLAYCVLSTPPYHWYYAPLISGMTVCA
                     VLVLGRVLAHRREVRLPVLAAVGFVVAVVLGFAMAWFDVDHGTPWQRAPISTNWATAA
                     QYERIGDDLRGIVGAGTVESPGEIGTLAYYCDCRIVDPFSDRGRVLGAIAAREEQSGW
                     LARTLIRLNYLHLDRTQQPRPVDARLVFQRGETPVGALQWPAYQWADGPGRMILLPGA
                     G"
     gene            22567..23460
                     /gene="rfbA"
                     /locus_tag="VFE39_01095"
     CDS             22567..23460
                     /gene="rfbA"
                     /locus_tag="VFE39_01095"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET6257222.1"
                     /translation="MKGIILAGGSGTRLWPITRAVSKQLLPVYDKPMIYYPLSVLMLA
                     GVREILLISTPHDVPQFRRLLGTGEELGLRIDYAEQSQPNGLAEAFVIGAEHVGEQPA
                     ALVLGDNIFHGPGFSGMLQNAIGDITAGRAGCALFGYPVRDPQRYGVAEADEDGRLLS
                     IEEKPEHPRSNHAVTGLYLYDNDVVEIARGLRPSARGELEITDVNRVYLERGTARLLD
                     LGRGFAWLDTGTHESLLEAGEYVRVLENRQGIRIACIEEIAMRMGYIDPAACYALGER
                     QATSGYGEYVMDVATSFKPRH"
     gene            23510..24490
                     /gene="rfbB"
                     /locus_tag="VFE39_01100"
     CDS             23510..24490
                     /gene="rfbB"
                     /locus_tag="VFE39_01100"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET6257223.1"
                     /translation="MQVLVTGGAGFIGSHYVRSLLSDAYPAYADADVVVLDLLTYAGT
                     LTNVAACADSPRLRFVHGDIRNADTVAEVMAGTDVVVHFAAESHVDRSIKGAAEFVST
                     NIVGTQVLLQAALEARVTRFVQVSTDEVYGSIDDGAWPETHALQPNSPYSASKASADL
                     LARSYHRTHGLPVCITRCSNNYGPHQFPEKVIPLFVTNLIDGKKVPLYGDGMNVRDWL
                     HVDDHCRGIQLVAEKGRDGEIYNIGGGTELTNRELTHRLIDAVGADASMIVSVPDRKG
                     HDRRYCVDWSKIADELGYAPRVPFDEGLAATVRWYADNRSWWEPLKKGQR"
     gene            complement(24471..25316)
                     /locus_tag="VFE39_01105"
     CDS             complement(24471..25316)
                     /locus_tag="VFE39_01105"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6257224.1"
                     /translation="MRALIIGAGGQVGRALAAALPQATALSRRGLDITEAAGADIDWS
                     SYDTLVNAAAFTDVDGAETTDGRVAAWQANAVGPASLAHVASEHNLTLVHLSTEYVFD
                     GRADEPYHEDAPIAPLSAYGASKAAGDLAVGTVERHYLMRPTWVMGEGRNFVRTMLAL
                     AARGVEPTVVGDQIGRPTFAQDIAAAIVHLVSKGAPYGTYHVTGSGEPASWAEVARAT
                     YALSGHGDLAITETSTEAYFADKPQAARRPLNSVLDLTRAERAGVALPNWRDRLADYV
                     SAALS"
     gene            complement(25317..25868)
                     /locus_tag="VFE39_01110"
     CDS             complement(25317..25868)
                     /locus_tag="VFE39_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET6257225.1"
                     /translation="MSGTLRVDETSIPGLLRIDLVVHQDGRGWFKEGFQRAKLEAQGF
                     PPFEVVQNNVSFNAEVGVTRGIHAEPWDKYISLAHGRAFAAIVDLRAGPTFGRSETFE
                     LHPGNSIFVPRGCGNSYQTLTPDVVYTYLVNEHWSPDASYTLVQAFDPALGIDWPIDE
                     EATIRSDKDRGHPPLADVMPMRV"
     gene            complement(25865..26857)
                     /locus_tag="VFE39_01115"
     CDS             complement(25865..26857)
                     /locus_tag="VFE39_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6257226.1"
                     /translation="MRAAVTGGAGFVGSNLVDALIADGAGVLVIDDFSHGSLLNLSDA
                     VGRGAELAELDVRDGAGVHRAMLAFRPDVVFHLAAQIDVRTSMLEPAVDAAVNVLGSV
                     NVFAAALAAGTRRVVNTSTGGAIYGETGLVPTPETTPADPISAYGLSKQTAERYGRWF
                     RRSHGLDVVTLRYGNVYGPRQDPRGDAGVVAIFCDHVLSGSRPRVFGDGSQTRDYVFV
                     GDIVAANRAVVAATDLSHDTYNVGTGKEIDVLTLVAAVAEAAGVEPTTFVPEFRPARA
                     GEVRRSCLDVSRARDELGLTASTDLMDGLRATLGWVRTMSPGEPTDVPPRGRAI"
     gene            26991..>27377
                     /locus_tag="VFE39_01120"
     CDS             26991..>27377
                     /locus_tag="VFE39_01120"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6257227.1"
                     /translation="MTAPEGRPRTDAPDYAERLQRLEQAHWKKWLDVQAPYRWNVRRL
                     DLGRVLDVGCGIGRNLMHLGGSGVGVDHNAASVAVARERGLEAYTSEEFLGGPQARKG
                     SFDSLLAAHVVEHMSEPDAVRVLQTYL"
BASE COUNT         3726 a         9651 c         9834 g         4166 t
ORIGIN      
        1 atcgagtgac ctatctgtca cgttggtaac gctctgtcaa cttttctacc gttcgtagac
       61 cgctcgtcct ggtgaggcga ccgttgatcg agcccgttgt cgcgcgcgta ggactacccg
      121 ctcagtggcc ccgctggagt attccgacgg gcgagtgtga ctgatctatc gttcgctgcg
      181 ttgtggtacc cggaaggcgc agcagcagga gtgcggtgcg ccgacggaca ggaacggaga
      241 ggacgccgtg agtacgacag gcgagcgatc tacaggcact gtatacgagc aagtgcaggc
      301 aagtccggag ttcgccgacc tacgctccag gctacggcgg ttcgtgttcc cgatgagtgt
      361 cgccttcctc gcatggtact tggcgtacgt gctgctcgcc tcgtacgcgc ggggcttcat
      421 ggcgatcaag gtgttcggca acatcaacgt cggcctgatc atcggactgc tgcagttcgt
      481 cagcacgttc gcgatcacca ccatctacgt ccgctacgcg gacaagcacc tcgacccggc
      541 ggccgagaag atccggcagc agatcgagag cgcggaatga gcatggtcgc gcaggccccg
      601 gccccgccgg cagcaaccgc cgtcggcagc gtccccctga acatcgggat cttcgtcgcg
      661 ttcgtcgcgg tcaccctggt gttcgtctac cgcgccagcc gcacgaacaa gaccgcgtcc
      721 gactactacg ccgcgggccg tgcgttcacc ggaccgcaga acggcgtggc gatcgcgggc
      781 gactacctct ccgccgcgtc gttcctcggc atcgccgggg ccatcgcgct caacggctac
      841 gacggtttcc tctactcgat cggctttctg gtggcgtggc tcgtggcgct gctgctggtg
      901 gccgagttac tgcgcaacac cggcaagttc accatgggcg acgtcctcgc gttccggatg
      961 cggcagcgtc ccgtgcgtgc cgcagcggcc acgtccacgc tggtggtgtc gttcttctac
     1021 ctgctcgcgc agatggcggg cgcgggcggc ctgatcgcac tgctgctgca gattcccacc
     1081 agcaaccggg tcggccaggg tgccgtgatc gccgtcgtcg gcatcctgat gatcgtctac
     1141 gtgctcgtcg gcggcatgaa gggcaccacg tacgtccaga tcatcaaggc ggtgctgctg
     1201 atcatcggtg ccgccgtgat gacggtgtgg gtgctcggca tcgtgggctt caacctctcg
     1261 gcgctgctcg gcgacgccgc cgccgcgagc cccaagggcg aggcgttgct cgcgcccgga
     1321 ctgcagtaca ccaacccgat cgacctggtg tcgctcggcc tggcgctggt gctcggcacc
     1381 gcgggcctgc cgcacatcct gatgcggttc tacacggtgc cgacggcgaa ggaggcccgt
     1441 cgctcggtgg tctgggcgat ctggctgatc gggctgttct acctgttcac gctggtgttg
     1501 ggctacggtg cggcagcgct actgcctcct ggcaccatca ccagcgccac ccagaacaac
     1561 gccgcgccac tgctggcgta cgaggtcggc ggcgagctcc tgctcggcat catctcggcc
     1621 gtcgcgttcg cgacgatcct ggccgtcgtg gccggtctga cgatcaccgc gtcggcgtcg
     1681 ttcgcgcatg acgtctacgc caacgtcctc aagcggggtg cggccacccc ggactccgag
     1741 gtgcgggtcg cccgcatcac ggcgctggtg atcggggtcg tcgcgatcgt gggcggcatc
     1801 atcgccaacg ggcagaacat cgccttcctg gtggcgctgg ccttcgcggt cgcggcatcg
     1861 gcgaacctgc cgacgatcct ctactcgctg ttctggaaga ggttcaacac caccggcgcg
     1921 ctgttcagca tctacggcgg gttggcgatc tgcatcatcc tgatcgtctt ctcgcccgcg
     1981 gtctccggca agccggtcaa cccggcaacg ggccgcagcg cgtcgatgct ccagggcatc
     2041 aacttccact ggttcccgct ggacaacccg ggtctcgtct cgatcccggc gtcgttcctc
     2101 ctgggcatca tcggcaccta cgtcggtggg cgacggccca tcgaggaggg caagttcgcc
     2161 gagatggagg tcagggcgct caccggagcc ggctcagaga aggcgatcgc gcactagttc
     2221 ccgaccggcg tcgacacgga acggggcggg cccgcgggct cgccccgttc gcgcgtcagg
     2281 agatcgtcac gatccgtcgg gactgcacgg cggcggccaa cccgatgtgc ggaacagccg
     2341 cgctccacca ccggtagccg cgctcgtcca cgaacgggtc cggctccggt tcggcgggga
     2401 gggcgtggaa caccgcggtc gcgaactggt cggcgtccgt cggccaggcc cccgagctcg
     2461 gatcgacgtc gaggcggaag cgggcggcga gcttgtgagc gcccacccgg taccggagat
     2521 agagcgccag gtccgtgccg acccatgtct cgtcgatcga tggcgggggc gtctcgatgc
     2581 cacggcagtg gaggcgacgc gccacggtca ccgcccattg ccccgtcaca ccggcgtagt
     2641 ccacagggtc cgaccgtagt ggtgacggca cccgttcggg tcatcgtcgt cgcggccgct
     2701 caccggctcg tcacgcggac gcgtggcagg atgccgtaca tgactggtcc agccgtcccg
     2761 gcggtcccgg tggcggatct gccgcccgac gccgcgctgc tcgacgtccg cgagatcgac
     2821 gaatggaatg cgggtcacgc gccaggggcc cggcacctgc ccatgtccga gctcacggcc
     2881 cggatcgccg aactgcctga ccacgacccg ctctatgtcg tgtgccgctc gggcggtcgc
     2941 tcggcgcgtg tggtcgccta tctcgcaggg cagggttacc ccgtagtgaa cgtcgaaggc
     3001 ggcatgcagt cgtgggccgc gcagggacgc gagctcgtcg ccgacggcgg cagcgggcca
     3061 gagatcatct agcaaccgtg ctccccaccg caccctcgcc aggggcggcc cggctcgggc
     3121 cgccctgccc ccgttgtggt cgacgggcac cggcgggggg cggtccgttc tgcccgtact
     3181 gcggtcgcta cctcgccgcg ctgcgatggg tcgccgagcc cccgccgtcc acgacgaagc
     3241 ccgcgcccgc gcgcgtccgc acccggtaca cgggtccgcc gcggtaccgc tcgctgcccc
     3301 gctggggctt cccaccgcgc gggtgggagc cgccggaccc ggccagcccg ccgcgatccg
     3361 cccccctgga cgccgctcgc tcgctctccg gcagtgcggt gccgctgctc tgggccacgg
     3421 ccgcggtggc gctgctcgcg gcttttgccg aggtatggcg ttatgtcctg ctgctggcca
     3481 gccgggacgg tgcgctgtcc gcagatgccg tggccgcgtc ggacgccctc gtcgcgtccg
     3541 ccggtacgat cgcgccgatc ctcacgctgg gcaccggcgg gctgctcgtc ctctggtccg
     3601 tgcgcgcgtc gcaggccgct gccgaccgtg ccggcgtccg cccgtcccgc tcggcgcgcg
     3661 ccatcgtgct cggctggctg gtgccgggac tcaacctcac ggtgccgggt tcggtcctct
     3721 ccgagatcga gcacagcgct ctgggtctgc ctgccgaccg gcgtccccgt ccgtcccgcc
     3781 tgctgctgat ctggtggggc ctgtgggcca ccggggtggt gctcgccgcc gtggtgctgc
     3841 tgtggtcgct gcgcgacggg gtgcaggccc gcgcggacgg ggtcgtgctg cacgccgtgc
     3901 tggacctgtt ggcggcggcc accgcaggcg tcacggcccg gctcgtcacc tggcacatgc
     3961 ggttgctggg gccgccccgg ccacgtcgcc gagaactggt gcggagcatc acgccgccag
     4021 ccgcagctcg cgcagcccgc gcatgacgaa gtccgggcgg cggtgcggtt cgtcggccag
     4081 ccgcatcccg ggcagccgcg tgcgcagcgc gtcgagcgca gcaaccacct ccagtcgggc
     4141 cagcggggcg ccgaggcagt agtgcacccc ggcgccgaag ccgaggtggg ggttgcgccg
     4201 cctgcccacg tccagctcgt cgggccggtc gaatgcctgt gggtcacgag ccgccgcccc
     4261 gagcagcgcc gcgatccgcg tgccggcgga caccgggaac ccggccaccg tcgtgtcgac
     4321 cacggcagtg cgctcgaaca gctggagcgg agggtcgtag cggatcagct cctcgaccgc
     4381 cgtggcgtcg agtccagggt cggccaccag ccgctgccac tgcgtggggt gacgcaggag
     4441 ggccagcacc ccgttgccga tcacgttgac ggtggcctcg tgcccggcca tgagcagcag
     4501 cgccgccgtt ccgaccagct cgtccgcgtc gacgtcggcc tccacgaggt gggtcaccag
     4561 gtcgtcgccc gggtgggtcc ggcgatgggc gacgagcccg cgcagcacgt ccacgaagtc
     4621 ggcggcggcc tgctccgccg cccgccggcg ctcctgcccc ggatccggct cgtacatcgt
     4681 gacgacggcg ttcgaccagc cgcgcaacgc cgagcgctcc ggctccggaa caccgagcag
     4741 ttcggcgatc acctcgaccg ggagcctcgc cgccactccg gacatgaggt cagcggcgcc
     4801 gtcggcgtcg atccgctcgg cgagctcgcc gaccagccgg tgcgccagcc cccgaaccca
     4861 cggctccagc cgcgcggtgt gaccccggtt gaacgcacct gccacgaggc cgcgcagccg
     4921 ggcgtgcgcc ggcccctccc gctcgagcag ggaattgcgg tgcagcaggt tgaacgccgg
     4981 gaagtcggcc gcgggctcgg cgtccaccca gatccgcccg agttcacggc cgcgcagcaa
     5041 ctcggagcag gccgcgtgcg acacggcgac cggcgtgccc agcagcgggt gagcgtgtac
     5101 cgggccgagc gcccgcagcg tggcgaagat cggatacggg tcggcgagaa agcgcgggtc
     5161 gccgggatcg aacggattca gcacaacctc cagtagcgac ggccggggcc acggcctacc
     5221 tgagccgact cggtcggcac agtgcgcgat ccccgtcggg tcgtacccgc tagcgtctgc
     5281 acaccatggc caagaagaca cgtgcccgtc gtgagggcac caccaaggac agcgcaagtg
     5341 ccgcgacgtc cgcgggcgag aacccccgtc gcccgtgccc ctgcggctct ggcaagcgct
     5401 acaaggcctg ccacggggcc ggcgacgacg tcatcgtcct ccggccgttc gagggcctgg
     5461 cggcggagtg cgacctggtg gcgatgcggg agttcctccc gtccgccacc gcgccgctgc
     5521 cgctgcgcga accgctcgac gtgtcggtga cgctcgcgac cgtgctgccg ggggcgtcgg
     5581 ccgcgctcgt ccgccccgac gggacgatca tgctgggcat gcaggtgcag acccggtcgg
     5641 gcgacctgtc ggcggatctc gccaaggccc tgcggtgggc acaggcggcc gagtcgggca
     5701 ccgcgctgcc cgtcgtcggg cccgggatcg gcgacggcga gctcgtccgg ctccaggacg
     5761 tgctcgatcc cgctgccacc ctggacgtca cgctgcacgc cgacttcggc tggtgggtcg
     5821 tgccggccga ggacggcgcc gagccttcgg ccgacgtgac ggccggcctc gagcgcgcca
     5881 acgccgtcat catgccgacg gagccggtgc tcgccgaggg ggtgcgggcc gcctactggg
     5941 tggacaccgg gacaaaggcg cacctgcgct gggtgcgtcc ggagcccgag gagaagctgc
     6001 tcgatgcact cgccaggctg cacgcccgcg gcgagctcgg cctcgccgag gatgcgcgct
     6061 tcgccggcgc tttccgggcc cacgggctgc tggtgccggt ttgggacctc gaccgggagc
     6121 tgcatcccaa ggagtgggcg tcgccggtgg ccacgttcgc cacgcggctc gatgccgcgc
     6181 tggccgacga gaccccgctc actgacgcag agcggcgtgc ccgcgatgcg ctcgccggca
     6241 agcaggtcac gctgcggtag gcgttggtcg aactcgaccg gtagcggctc gcgccgagtc
     6301 cgagtcatat agtccgatca tgggacttct cgatggcaat gtgatcgtcg tcaccggtgc
     6361 gggccgcggc atcggtcgtg gtgaggcgct ggagtgtgcg gcccaggggg ccgctgtcgt
     6421 ggtcggtgag ttcgacccgg acgcgggcaa ggcggtggcc gacgagatca cggcagccgg
     6481 cggccgggcc gtcgccgtga tcggggatgt cgccgacgct gccgtggccg actcgctcgt
     6541 cgctactgcc gtcggcgagt tcggtgccct cgacgccctg gtcaacaacg ccgggatcct
     6601 gcgcgaccgg acggtcgcca agctctccga cgaggaatgg gacgacgtca tccgcgtgca
     6661 cctgcgcgga cacttcctgc ccacccgggc cgccgtgcgg tactggaagg aggcgaagcg
     6721 gccgggccac gtcgtgcaca cggcctcgac ctctggcctg ctcggcaact tcgggcaggc
     6781 caactacggc gcggcaaagg ccggcatcgc cgcgttctcc acgatcgtcg cgatggaggg
     6841 tgcgcgcggc ggcatcacga gcaacgcgat cgcgccgact gccctcaccc ggatgacgct
     6901 cgacctgatg cccgaggagt tccgggcgga gcgcgaggcc tcgatcgcgc gcggcgagtt
     6961 cgacttcttc gccccggaga acgtcgcgcc gttggtggcg ttcctgtgtt cggacgcctc
     7021 gagccacatc agcggcaagg tgttcggcgt gcagggcgac agcgtcgaga tcttccagcc
     7081 gttcaccagc gttgcggaga tccggaacga cgggaagcgc tgggacccgg aggacctgcc
     7141 gggccggatc gacgagctgt tcgacgcagc gggcgtccag gcgggcccgg agaacatgat
     7201 ggagcggatg cgctaccaga tcctcgccgg gcgctgagtc gttcagggca gccagcccac
     7261 cttcccggcc agcacggcgt agccggtgaa ggcgacgacg tcgatcaggg cgtgcgcgcc
     7321 gaccagcatc cacaggcggt tggtgcgctg ccacacccgg ccgaacacca gccccatcac
     7381 gaggttgccg atgaacccac cgaggccctg gtagaggtgg taggagccgc gcagcaacgc
     7441 ctgggccacg agcgagcggt tctccgacca tccgagctgg cgcaggcggg tgatcaggta
     7501 gccgatcatg accacctcct cggcccagct gttggcagcg gccgagagga tcagaacggg
     7561 cagccgccac cacgtgtcgc cgagcgtgga cggcgcgatc gtcagactca ccccgatcgc
     7621 tcgcgccacc agatacagac cgaggccggg cacgccgatc agcgcggcca gcccgaccgc
     7681 accgagcgcg tcccgacctg ggcggcggcc gtccagtccg acatcgcgca gggcgatccc
     7741 cgccctgatc agcaggtacg cgcccagcgc tccccagccc acgagctgca gcacacgcgt
     7801 gagctgcagc gccaggtcga cgagtgcggc ctgcgcttcg ggagcgttga gcgccacctg
     7861 ttgcttgttg agcggcaccg tctggagcag ggcatcgagc aggctcaggg cgctgcgtag
     7921 cgcggacagc ccgagggtca ccgtgaggac gacgagcacc tcggtgccga cgaggcgccg
     7981 ggtgcgcccg tcggtgaccg gcgcggccac gggtgacggc ggcgacagcc attgcctggc
     8041 cgtcgtcgag cggaggctgg acacgatggc ggacggtacc cggcgagtaa gctgtcggcg
     8101 tgcgacagct ggtcttctcc ccccgctgga tgctgtggca cgtgctcacc ctcggggcca
     8161 tggccacctg cggctggctg gcggcctggc agtggcaacg tgccggatcg gccatggggt
     8221 cggcgctcaa cgtcggatac ggcctgcaat ggccggtgtt cgccgtcttc ttcggcgtga
     8281 tgtggtggcg catgctccgg atggaggcgg cgaagctgca ggagggcgag gcggacgtgg
     8341 cgggcgaagc ccctgcgcct gccgggggag gaagcgctcc ggtcgtggcc ggacaggccc
     8401 gttccgccgg gccccgatca gccgagcccc gcccgcgtgc cgcatccgac gcaccgtcgc
     8461 cgttcgggcc acgtcccgca tcggtgctgc cccgggtcgc cgcggaggac gacccggagc
     8521 tggccgagta caaccagatg ctggcccacc tggccgcccg tgattccacc gactgacgaa
     8581 ggacgtccgt gtcgatcgag accgctctgc ggaactaccg cgtaatcgcg tgggtcaccg
     8641 gtgtgggcct gctcgtgctc gtgctggtgg cgatgccgct caagtatttc ttcggtcagc
     8701 cgcagctcgt cgcgctggtc ggcatgacgc acggcttcct gtacatgatc tacatcgtct
     8761 gcaccttgct gctggccgag cgctgccggt ggaagccgct cgacgcgctg ctcatcctgg
     8821 tcgcgggcac ggttcccttt gcgtcgttcg tggccgagcg ccgggtgacg caccgggtgc
     8881 gggcgggccg ttctccggtc agctagcggt tccgtcgagc ggacccgcgt ccgtgccgtt
     8941 gaccggcgca gccgaccagc agccccatct gcacgcgcag ctcgtccacc gtggttggag
     9001 tgtcctgcca ggcgaggcgg acgtcgtggt ccctgcccgg gttctcgaca ccgtccggga
     9061 cctcgatccg caaccgcagc ccacaacggt ccgcaccgag tgggcgcatt cgtgtgcccc
     9121 gaaggccgag cagcgggggc ggcaggtgcc gggccagtgc ggcgagcacc tcggggtgtg
     9181 cctcctcgag gtgggcgagc cagcgctgct cgtagcggca gaaggggtcg ggacgcgccg
     9241 ccgccagctc gatcggagcg agtgcggcgc tgccctcggc gtcggacagc acggcggagc
     9301 cgggatcgag ccgcacgagc gtggcgccgt ggccgagctg cagcaggtcg ggatgcggcc
     9361 gcacgtcggc cacgtgcacg gcgagcctgc gggcctcctc caggtgcggg agtcggagcg
     9421 tgccggtgat ccagagcagt gcgcgcaccg gttgcctgag gtcgaccggt gcgtggtctg
     9481 cgagctcgag catcgccgag gacgtgccgt caggtgcctc cctgatccgg tcgaggaccg
     9541 ggtcgtcgtc ggggagcagc agcacagcgg acgcgtcggc gcgcacgtgg tgcaccagtg
     9601 gtgcggtccg gggcgctccg gtgccgacaa ggagggccgt gccaccgcgg gcgaccacgc
     9661 tgcgagcacg ctcggcatcg gtgggcgccg ggggtcgtcg cagccgggtg gattccaacg
     9721 aagtcacctc cgaattaggt tagcctaagc tatatccacc cgttcggaac tgtccagcac
     9781 tcgggaaccg gcagccgttg tgaagcgctc cgaggcgacc cgttagcgtc tgggaccgtg
     9841 acgacggcgg caggtgtgaa ctcctcctgg gccatcccac cgctcttcgc ccatctcgtc
     9901 gacgacacga gtctgctgca gccccgttcg gtcgcggtcg gcatcgacaa ggtggtctcg
     9961 cggtacctca gcgccaggga cggtcgctac ggcaatctcg tcggacagct cgtctgcccg
    10021 gtgtcgcggc tgcccgcgct cgtggccgag ctggcgcgcg cagtcccgtc ccgtcccgtc
    10081 gagctgtcgc tggtcgtgga caccgggctc ggcggggtac cgaaggcgct gtccaccgtc
    10141 ttctcccgat cgagcctgct caccgcgcac accgtcgaga cggcggcgcc tcccgacgtc
    10201 gacgcggtct ggctggagcg cgtggccgag ttcgtgcccg aggacgtcgt tgccgtcgtc
    10261 gagccccgca ggccgtccgt cgccgagccg gtcggcaccg gcgcgtggct ggacgcggtg
    10321 cgccgggtgg cggagcacgg ctgttcgccg aagctccgtt gcggtggtcc gcgggcctcg
    10381 gacgttccgt ccgtcaccga catcgaggag ttcgtgcgcg tcgtggtcgg ggcagggcgt
    10441 gggttcacca cgctcggcct gcgccaggtc gtccacacgg acgacggcac ggtcccctcc
    10501 cagcacggcc tcctcaacct gctcgtcgcc gtcgcgaggg cgctggcggg cggtgacgtc
    10561 cgcggggccc tggagactac cgacggtgct gcgctcgcgg tggagatcgg cggactgtcc
    10621 gagcaggcgg tcacgggtgt ccggggtctg ttgtcgcgct gcggcgccga ccccgagccg
    10681 gttccggccg cgcagctggc aggcctgggc ctcctcgcct gagtcgcgtc gcggcggtca
    10741 ggcggggccc agcacctcgt ccagtgcggc gccgacctgg tgcgcgcaga cgtctgggtc
    10801 caggccgccc actccggtgc cgaggccggg cagggcgatc gtgcggatcg cctcgcggac
    10861 cggggtgccg ttcccgggca gggccccgtc ccgccacagc cggagcaccg ccgatgcggc
    10921 cgcccgtgcc gcgcctccgt cggcggggag cagctcgccg ggccggcgca tcgtcggagc
    10981 gctgatcaac caccccgggc gggcaacgcc ggtcggcacg atcaccgcat cgccgacggg
    11041 gagctcgccg ccccggtccg ccccggacgc ggcgcgcacg cggtcggaga tccgggggag
    11101 ccagcgcgcg tacaccccgt cgatgccgcc gcccatgacg ccgagggagt tggccgggct
    11161 gaccacggca tcggcgtcca catcggtgat cgagccctcg tgtaccacca gtcccggccg
    11221 agtgccggcg agcgtgcgcc acgccgccgc catcggctcg tcgacggcca cgagaaccag
    11281 cgtcaggtgg gtcacagcgc cacagtaggg tggtcatccg tgccccgcct cgcgtacctc
    11341 gggccgcgtg ccaccttcac cgagcaggca ttgcggtcgc tccccgagtc ctccggtgcc
    11401 gagctggtcc cctgcgccgg gagccccgca gtgctggccg ccgtgcggga cggatcggtc
    11461 gacgccggct gtgtcccgat cgagaacacc gtggagggag cggtgccggc ggtgctggac
    11521 ggcctggtcg ccgacccacc gctggtgatc gtccgcgagg cgctggtggc cgtccggttc
    11581 gcggtactgg tccgcccggg cacccgggcc gccggtatcc gcaccatcgc ctcgcacact
    11641 cacgccatcg cgcagacccg cggctggatc gccgcccagc tgcccgacgc cgaggtgctc
    11701 ctgtccacct ccacggcgga agccgcggcc caggtggccc gcgcggagtt cgacgccgcg
    11761 atcgccgcgc cgctcgccgc ggagcagcac gggctcgagg tgctcgtcga cgacgtcgcc
    11821 gacaatcccg gcgctgtcac caggttcgcg ctgctggccc cgccgggtcc gccgcccgca
    11881 cccaccggtc aggaccgcac gacgctggcg gccaccacgc agaaccggcc cggtgcgctg
    11941 ctgggcctgc tcaccgagct ggcggtgcgc ggcatcgacc tgacgcggat cgagtcgcga
    12001 ccgatcaagg accggcacgc ggagtactgg ttccacctcg actgctcggg ccatgtcgcg
    12061 gagcccgcca tgggggaagc actcgccgcg ctacgccggc gctgcgaccg ggtccgcttc
    12121 ctcggctcct atccgcgggc cggaaacggc cctcagcaga cgtgcggctc cgacgctccg
    12181 cccgtgccgg tcgggggcgc gggcgcagcg gagttcgctg cagcacagga gtggttggcc
    12241 gggctgcgcg ccgggagcgc cacgtgaccc ccggtccgct gcgcctggtg ctcgtcaggc
    12301 acgggcagac cgatgccaac gtgcgccgcg agctcgactc cgtgcccccg ggcgcaccgc
    12361 tcaacgagct cggtctcacc caggccgcgg cattggggga gcggttgtcg acgttcccgg
    12421 tcacccgggt gcacgcctcg cgggccaccc gcgcgcaaca gaccgcggca ccggtggcgg
    12481 cggcgcacgg ggtcgacgtc gccgtgctcg acggggtgca cgaggtattc gtcggcgatc
    12541 tggagggccg ctcggaccga ccggcgcggg agcagttcaa cgtcgtctac ggggcgtggt
    12601 ggaacggtga tctggacgcg cggctgccgg gcggggagtc ggccaaggac ctgcgggcac
    12661 ggtacctgcc ggtcgtcaag cagatcacgg acggcgcgca cggagacgtc gtgctcgtca
    12721 gccacggtgc ggcaatccgc ctcgcggcgg cggcgctgct gggcgacacg gcggagacgc
    12781 tgtacgtacc gaacaccggc ctcgtggtgc tggccgcgga tccgtcggcg cccagcggat
    12841 gggcgctcga acactgggac agcgcggtgc ccgttcccgg tgacgtcacg gcaggcggga
    12901 cggcgggctg accgccccgg ggcggtcagc cccagcccag ctcgcgcagt gtctcctcgt
    12961 tgatcccgaa gtggtgggcg acctcgtgca ccacggtgat cgccacctcg cggacgacct
    13021 cgtccacgtc gtcgcacacg tccaggatcg cgtccttgta gatcgtgatc cgatccggca
    13081 gcaccccgcc gtaggacgtg gtgcgctcgg tgagtgcgac acctacgtag aggcccagca
    13141 ggtcgggctc ctccgcgttg cggtcggcca ccagcaccac gacgttgtcc atcgcggcgg
    13201 tcagctgtgg tgggatctcg tccagcgcat ccgccacggc ctcctcgaac cgccgccggc
    13261 ccatctccat gtcgaggacg tcaggccccg gcgggcggcg tcggcggcgc tggtggggtg
    13321 aacgacggcg gcgtcggcgg gttgatggac ggcctagccg gcgggccagg aggcaccacc
    13381 ccgccggacg gctccggttg ctcgcccgca ggcggctcgt cgacggggcc ggggatctgc
    13441 tcggcaggcg gaccgtcggt cggctcgggg gagggggtgg tggtggtggt cggcgccggg
    13501 acgcctgcgc ccgtggtgtg gggcgcgggc ggagccgctg tgtcgcccac caccacggca
    13561 ccccgcacgg gaccggtgac gggcgcgaat ccgctgcggt ccggcaggag cgccgccagg
    13621 ttgcagttga ccttgcgggt accggccttc caggagtcct cggtgaggtt gtcccagtac
    13681 accgtgagct tcttggccgc gatcacgtcg gccccaccgg cgtagtcgtt ggcgatcttc
    13741 ccgcactcct gctggaggaa cggatcctgg tcgccgacgg cgggaaacgc gccggggaac
    13801 ttgccggaga ggtcgacgat cccgaccgtc tcgacggcat gcggcccggc gcagtccacc
    13861 gggtcgccga tcgtgcggcc atcgatggcc aggcaggtgc cgggatcgtg caccgcggac
    13921 tggtcggagt cggcgacccg cccggccagc gggtacagcg caccggagcg cgacgcgctc
    13981 tgcagtccac agcgcagctc ccggtcgccc tccgcccact tcgtgggcga cggcttgagc
    14041 gcgcccaccc ggtagcggcc gtcggggtcg aacttcccgc ccaggtagtc gagtacgggc
    14101 tggttgcacc gctcgttgac gagctggcgc cactgccggt cgtcgggcag cgcggtctgg
    14161 tcggtgagcg tcaccgaggc ggcctgttcg aaaaggtgcg gctgggcgca gtcgacgacc
    14221 ttggtgtcgg ccgcgtcggg acgagtccag ttgaggcacg tgccgggggt ggccggcggt
    14281 gccggcgcct ccaccacgtc gggctcaccc gctatgccgg gcagcgccgc aagggacccg
    14341 aggacgggca ccgtggctcc ggcgaacgtg tccagcgtgg ccaccccgag catcgtcagt
    14401 gcgcccacga ggacgcccaa aacgatccgg cgggccgggg gctgcttcgg ggagcggccg
    14461 ccgcttccag gcggtggcgg ccggtcggcc ggagcgggct gcggccggcg gggagacccg
    14521 tgacgagcgg cgcgcggggg agcgccgatg cccgccgggg cggcggccgg tcggctcggg
    14581 ttccacgagg gtgcgacgcg cggcccggcg gtgcttcgcg gggcagcggg gcgcacggca
    14641 ccagcgccat gggtggcggg ccggcgggga gcggcgagga gtcggtccat gtcggccgtc
    14701 atcatgccgt tacctctggt cggaccagga ggcgagacgg cgaccgcgcg accatcggtc
    14761 gtcggcgtgc tgcgaacgtc agcctttgcc ggtttgcgta ccccaggtac ggttccgccg
    14821 gcagggtgga cctgtgaacg gccgaccggt gcagccgagg gggatcgagt gccaaccgac
    14881 gacgaccggc ccggcggcga cgagcccggc gaccggcgcg agccacccac cgggcggatg
    14941 cgcttccagg acgagtcgac cacgccgcgc gagcccacac tcgccgagcg ccgcgcgcgc
    15001 gagcaggcgg cgcgccgcga ggaggaggcg gagcgccagc aggccgagga ccaggagcgg
    15061 tcgcgcaaga agcgcaagcg gatcctcatc ggcgcggggg ccacggtggg cgtcgtcgcg
    15121 ctgatcgccg tcgggtactc cgtggcgcag cccgacaacg aggtgcaggc gcaatgcgtc
    15181 gacgagcaga cgaaagtcgt cgtcgacgac gccaactgcg tgacgccggc gagctccggc
    15241 ggcagctact acggcggggc cggcatcgtc ccgtacccga tcttcctcgg cggcggtggt
    15301 cgtcagtacc gctacaacta cggtggcagc gggaacatcg gccagccggt cagtggcggc
    15361 acctcggtgg tgcccaaggc cggtacccgg gtgaccactc cctccggcaa gagcgtcgcc
    15421 ggtacgtcgg gcagcggctc ggtctcacgt ggtggcctcg gcgtgtccaa cagcggctcc
    15481 agcggctcgt agacggatgg aacaaccctg agacatggaa cgacgtaggg gggagccccg
    15541 gccggactgg gagaccacgg tccgcgacca gggaatggtg tacggcacgc ccggccgggg
    15601 ggagggcggc gcgccacggc cgtactggga cgagtccgtg cattacgtct tctcgatgga
    15661 cgagatcctg tccctcgagg ccgacgtcga ggtggtccac tcgatgtgtc tcgaggccgt
    15721 cgaccacgtc gtcaccaccg agcggttccg tgacttcgcg ctgccggagt ggtcctggga
    15781 gggcgtcgcc gcctcgtggc gacgcagcga cccgcacgtc tacggccgct tcgacctgcg
    15841 ctacgacggg ggtgggccgg ccaagctgct ggagtacaac gccgacacgc ccaccacgct
    15901 gctcgaggcg tcgatcctgc agtggaactg gctgaaggac actcacgagg gcgacgacca
    15961 gtggaactcg ctgcacgaga agctcgtgga gcggtgggag ctgatcaagg cgcggctgcc
    16021 cggtgacgag gtgcacttca cctggtcgag cggcgactcg accggcgagg accacgtgac
    16081 cgtcggttac ctgcaggaga ccgcagccga ggccgggctg aacaccgtcg ggctcgcgat
    16141 cgaggacatc ggctgggacg ccgcgctcga ccgcttcgtc gatctcgagg aagcgcccat
    16201 ggcggcggtg ttcaagctct atccctggga gtgggtgctc accgaccagt tcggcaagca
    16261 cgtgttgcgc agcctccccg agacgctctg ggtcgagccg ctctggaaga cattgctgtc
    16321 gaacaaggcg ttgctcgccg tgctgtggga gatgtacccc gggcacccca acctgctgcc
    16381 tgcctacctc gaccagcccg gcctgctcac cgagtacgtc cgcaagccac gcctgggccg
    16441 tgagggcgcg aacatcgcga tcgtggctcc gggtatggag caccagacgg gtggcgtgta
    16501 cggcgaggag ggatacgtct accagctctt cgatccgctg cccgagttcg acggctaccg
    16561 tcccgcgctg ggcgcctgga tcgtcggcga caccgcggcg gggctcggca tccgcgagac
    16621 cgtcggcctg gtcaccgacg acggcgcggc gttcgtcccc catcgcatcc ccgaaccctg
    16681 atgctccctc tggaggtttc cgtgacgctg gcccagatcg acccggggtt cttctcggtg
    16741 atcggacgcg ggatcggcgc gatcctgctc tatgccgtgc tcggtgtgct gctcatgctc
    16801 ctcggcttct acgccgtgga cctgacgaca ccgggcaagc tgaaccgcat ggtccgtgac
    16861 ggcctgccca acgccgtcct cgtcaccgcg gccggcatgg tcagcatggc gttcatcgtc
    16921 gtcacggcga tctactcgtc gtcgggagcg ctcctcgagg gactgctggc ctcgctgatc
    16981 ttcggactgg tcggcatcat cgcccaggtc ggcggcgtgc ggctgctgga gtgggtcacg
    17041 ggcatcgaca tcggcgcggt gctggccgcc gagcgcgtgc agccccaggc gtacgtggtc
    17101 tccgccgcgc acctggcact gggcctcgtg gtggcggtgg ccatcctctg accctcgtgg
    17161 cttcggccac cgagcggctc gtagagcgga cgtcggcatc acggttgact ctcgaccgtg
    17221 tcgagacacg agggtcgctg atcgtggaga gcttgcgcgg gatcgggcgg atgtcccggg
    17281 agagcggcct tgccgtcagc gccctgcggt tctacgacgg cgccggcgtg ctcgtaccag
    17341 cccatgtgga cgcgcggacg ggctaccgct cctacgcgcc ggatcaggtg ctcgcggccc
    17401 ggctcgtcgc cgtgctgcgc cgggtgggca tgccgatcgc cgggatccgc ggggtgctgg
    17461 catgccgaca cgatcccgct gccgtcgacg agttgctggc cgcgcacctg catcgcctgg
    17521 agcaggggct tgtcgacgcc cgccgcgagc tctcctccgt caggtccctg atcgagcagg
    17581 aggaacatcc cgtgtcccag accaccgtca ccgtccgcgg ccacgaactg tcggacgcgc
    17641 tgcgcgccgt ccggttcgcc gtcggagccg atcccgagct gcccgtgctg gccggcgtgc
    17701 tgctggacgt caccgcagag gcggtcacgc tcgtcgccac cgaccgatac cggctcgcgg
    17761 tctcgtccgc gcccgtgctc acgctcgacg ggccaccggt cagggccgtc gtacctgccg
    17821 ggttggccga ccggattccc gacgcaggcg tcgtgtcgat cgccgtcgac ggaaccacca
    17881 tcacggtgct gtccgacgac gacgtgctca gcggggcgct catcctcgac gacttcccgg
    17941 actaccgcag actgctcggc gacgtgggcc ggccggtgcc ggtcgggcgc gcgctgcgag
    18001 cggcgctcgc agcgggaccg gtccggacga tgcgccgcga gcaggacggg gcggagttcg
    18061 cggtggcggt gctcgggccg accccgaacg gcgggatcac cgtcgacccg gtcggtgacg
    18121 gcgtcggggt gaaccgcgag ttcctgctcg aggcactcga cgcgggcggc gagggccagc
    18181 tgctgctctc cctggacggg ccgatcgcac cgctggtcat ccgctcgtcg cggtcggtgt
    18241 cactgctgat gccggtgcgg ctgtgaccgc gccggacccg gggacatgat ccggggcggc
    18301 atccgccggc tgcggacgga actcggagcg ggctgaccgc tctccaccag cgtcggggcc
    18361 ggcggcgcag accgtaggct ggtgttgtgc aagccccccg cctggtcgcc acggatgtcg
    18421 acggcacgct gctcgacccc gacgaccggg tgagcaggcg cgccgccgcg gtgatcggca
    18481 gggcggttgc ggctggagtg catttcgtgc tggtcaccgg tcgtccaccc cggtggattc
    18541 cgccggtggt ggatcagctg ggggtcgcgc gcatcgcggt gtgcgccaac ggcagcgtgc
    18601 tctacgacgc cctcgaggac cgggtgctct cgtcgcacac gctcgacccg gccaccctcg
    18661 cggaactcgc ggaggccgcc gcggtggcgc tgcccggctg cgggctggcc gtcgagcgcg
    18721 tcggggcggg ggcgttcgac gactccgcac cgttcgtcgc cgagccgaac tacctgcacg
    18781 cctggccgga tgccgacaac gccgtggccg tgcggtcgga gctgctgtcg cggcctgcgg
    18841 tcaagctgct cgtccggtgt ccgcagctgt cgagcgacgc gatggtcgcg gcattggccc
    18901 cggtggtcgg cgatgcagcc gacctgacgt tctcgcaccc ccgcgggctc gtcgagatct
    18961 cgccgcacaa cgtcaccaag gcgaccgggc tcgccggggt ggtccacggg cacgggctca
    19021 ccgccgccga cgtcatcgcc ttcggggaca tgccgaacga tctggagatg ctgcgctggg
    19081 ccgggcacgg cgtggcgatg ggtaatgcac acccggccct gctcgacgtg gccgacgagg
    19141 tcacggcgag caacggcgac gacgggttgg cactggtgct cgaacgctgg ttctgaccac
    19201 gcgaggcgac gtacggtgtc ggatcgcacc gctcccggct actcctggtg accgggcgta
    19261 gcatcagggc ccaacacagc gcatcgcacc gagcggcggg cactcgcccg tgacaccacg
    19321 ccgcaccgac cggcgcgggc cagccccgcc acgccacaat ggaggccccc gcaccgtggc
    19381 cgagctcctc gaccgccctg gcccgacgcg cgtccccgcc gtcgccccgg agagcactgt
    19441 cgacgtccca cccgccgccc tcgaggtgcg cgtcctcgag gccgtgcagg cccggatcgg
    19501 cagcccgccg gtcgtgcggg tcgcacgcgg gatgtccctg ttcggcgagc atgccgcggg
    19561 ctggctcctg ttcggcgctc tcggcgcgat gatcgaccgg cgccggcggc gcgactgggt
    19621 tgccgccacc gcggccgtcg ccgtggcaca cggggcgtcc gtcggggtca agcgtgtggt
    19681 gcgcaggccc cggccggaca acccccgcgt ccaggtgctc gtcggcaccc cgagcaagtt
    19741 gagcttcccg tctgcgcacg ccacctccac cacggcggcg gccgtcgtgt tcggcgccct
    19801 gctacgcaag ccgctggcgg cggcgctggt gccgccgatg gccctgtcgc gcatggtgct
    19861 gggcgtgcac taccccaccg atgtccttgc gggctccgta ctcggtgccg cggtagcggt
    19921 cgccgcccgg cggctggcgc ggcgtcccac tgcactacgc gcccaggagg ggaacgaatg
    19981 accagcacgg acgtgccgac caatcaggcg gcatcgcccc gcaccccggt gagcgtcacc
    20041 cgcggcgtcc tcaagacgat gcggccgcgg cagtgggtca agaacgtcct ggtgctcgcg
    20101 gcgcccttcg tcgccggcga cctgttcacc gcgggtatcg cgatccacct cgcggtcgcg
    20161 ttcgtggcct tctcactggc cgcctcgggc atctacctcg tgaacgacgc aaacgacgtc
    20221 gccgcagacc gggcgcaccc gaccaagcgc aaccgcccga tcgcagcggg catcgtggct
    20281 cctcggctgg ccctgtcggt ggccgtcgtg ctgctgctcg gtgcgctggc gctgagcctg
    20341 ctggcgagcg tgcagctggt gatcgtgctc ggcgtctaca tcgcggtaca gctggcgtac
    20401 tgcttctggc tcaagcacca gccggtgctc gacatctgcg tcgtcgcgtc gggcttcctg
    20461 ctccgggcga tcgcgggcgg cgccgccacc gggatcgcgc tgtcgcagtg gttcctgctg
    20521 gtggcgggct tcgggtcact gttcatggtg gccggcaagc ggtacgcgga gatgatgctg
    20581 gccgaacgca ccggcgcgaa gatccgcaag tcgttggaga gctactccgc gtcgtacctg
    20641 cggttcgtct gggcgctgtc ggccaccgtg ctgatcacga cgtactgcct ctgggcgttc
    20701 gagatccgcg cggagcggca caacaccgtc tggtcggtga tctccatcgt gccgttcgtg
    20761 atcgcggtgc tgcgctactc cgtggacgtc gactccggca acggcggcga gccggaggag
    20821 ctcgcgctgg ccgaccacgt cctgcaggtg ctggcgctgc tctggatcgg cacgctcgtc
    20881 ctcgccgtct acgcctgacc cgagggcacg gccctcccag cggtccgcca cagtgccgtg
    20941 gcacggaccg gtcctacgtc cccgtccgcc gaggaggtgc tggatgacat ccgctcgcgt
    21001 gatcgacccg agttcggtgc gtgtagcggg ccgccgtgtc gcgactgtcg tcccggacct
    21061 cgtgatgctg gtgggtgtag ccctcgcggc gagcgccgcc ttcctgctcg tccatcgcgc
    21121 cctcatcgat gacgcgtaca tcacgatgac gtacgcccgg aacctcgcat tccatctcca
    21181 ctggggactg atcaccgacg agacggccaa cagcgccacc tctcccctga acgtgatcct
    21241 gctcggggcg atcaccttcg tcctgcggaa cgcacagatc gcggtcggcg tgctgttcgt
    21301 cgcctccgtc gtgctcgcga cgcggtggct caccactctc acgacgctgc tcgggctgag
    21361 ccgtgcaacc ccgtacctgg gcgtggctct gctcctgctc aatccgctcc tgctctccac
    21421 agtggggctg gagtcctacc ttgcggccac gcttgtcatc ggcctgctcc ggttcggagc
    21481 cgagggccgg agcatcgcct tcggggtggt gagcgggctg gctttcctca cccgcccgga
    21541 cctcgccgtc gtcgtcctga tcgcagccgt cgggtttccg ggcatccgcc gtcgggtcgt
    21601 ccgtgcgttc gcggtggcgg tgctggttgc gggtccttgg cacctgtggg cctggttcgc
    21661 gctgggctcg gcactgcccg atacgctgat catgaaagcc ggctcgccga cgtggtccgg
    21721 cttcggcttc tgggacggcg cgctcatgta ctggggcggc gctcccatcg tcacggcact
    21781 ggccttccta cccgtcctgc tcggagccgc cgcgctgcta gcggtgctgg tggcgcgact
    21841 tttcggccac cgggacgcgg cgcatcgcac cgcagccgtg gccggtgtcg gtgcaggggc
    21901 ccactacctc gcctactgcg tcctgagcac tccgccctac cactggtact acgcacccct
    21961 catcagcgga atgacggtgt gcgcagttct cgtcctgggc cgggtgctcg cccatcggcg
    22021 ggaggttcgg ctccccgtgc tggcggcggt aggtttcgtc gtcgcggtcg tgctcgggtt
    22081 cgctatggcc tggttcgacg tggaccacgg aactccatgg cagcgagcac cgatctcgac
    22141 gaactgggcc accgcggcgc agtacgagcg catcggcgac gacctgcgcg gcattgtcgg
    22201 agcaggcacc gtcgagagcc cgggggagat cgggacgctg gcctactact gcgactgtcg
    22261 catcgtcgac cccttctccg accgcggccg ggtgctcggg gcgatcgcgg cgcgggagga
    22321 gcagagcggc tggctggcgc gcaccctgat caggctgaac tacctgcatc tcgatcggac
    22381 ccagcagccg cgcccggtcg atgcccggct ggtgttccag cgcggggaga cgccggtggg
    22441 tgccctgcaa tggcccgcct accagtgggc cgatgggccg ggccggatga tcttgctgcc
    22501 cggcgccggg tgatcgcact ccccgcacga cagcgtgatg ggcgcggcgt gacacgatct
    22561 gcggcgatga agggcatcat cctggccggg ggctccggaa cgcgcctgtg gccgatcacg
    22621 cgcgccgtgt cgaagcagct cctgccggtg tatgacaagc cgatgatcta ctacccgctc
    22681 tcggtgctga tgctcgcggg cgttcgggag atcctgctca tctccacgcc gcacgacgtg
    22741 ccgcagttcc ggaggttgct cggcaccggt gaggaactgg gtctgcgaat cgactacgcc
    22801 gagcagtcgc agcccaacgg cctggccgag gcgttcgtca tcggtgcgga gcacgtgggt
    22861 gaacagcccg cggcgctcgt cctcggcgac aacatcttcc atggccccgg attttccggg
    22921 atgctgcaga acgccatcgg cgacatcacc gccggccgcg ctggatgcgc cctgttcggc
    22981 taccccgtac gcgaccccca gcgctacggt gtggcggaag ccgacgagga tggacggctg
    23041 ttgtcgatcg aggagaagcc ggagcatccg cgctccaacc atgccgtcac ggggctctac
    23101 ctctacgaca acgacgtggt cgagatcgcc cgcgggttgc gaccctccgc gcggggtgag
    23161 ctggagatca cggacgtcaa ccgggtctac ctcgagcgcg gcaccgcccg cctgctggat
    23221 ctcgggcgtg ggttcgcctg gctcgacacc ggcacgcacg agtcgctgct ggaggcgggt
    23281 gagtacgtac gggtgctcga gaaccggcag ggtatccgga tcgcttgcat cgaggagatc
    23341 gcgatgcgga tgggctacat cgaccctgcg gcctgctacg cgctgggcga gcggcaggcg
    23401 acgtcgggct acggcgagta cgtcatggac gtagccacat cgttcaagcc acgacattga
    23461 gcaagacgcg tcgagtccct cggagcgggg gacttacgaa aggagcctcg tgcaggttct
    23521 cgtcaccggc ggtgcgggct tcatcgggtc gcactatgtg cggtcgctcc tgagcgacgc
    23581 gtacccggcc tacgccgacg cggacgtggt cgtgctcgac ctgctcacct acgccggcac
    23641 gctgaccaat gtggcggcgt gcgccgactc cccgcggcta cggttcgtgc atggcgacat
    23701 tcgcaatgcc gacacggtcg ccgaggtcat ggcgggcacc gacgtcgtcg tgcacttcgc
    23761 ggccgagtcg cacgtggatc gctcgatcaa gggcgccgcc gagttcgtct ccaccaacat
    23821 cgtgggcacg caggtgctgc tgcaggccgc gctggaggcg agggtgaccc gcttcgttca
    23881 ggtgtccacc gacgaggtgt acggctcgat cgacgacggt gcatggcccg agacacacgc
    23941 actgcagccg aactcgccgt actcggcatc gaaggcgtcg gcggacctgc tcgcgcgctc
    24001 ctaccaccgc acccacgggc tgccggtgtg catcacgcgc tgctccaaca attacgggcc
    24061 gcaccagttc ccggaaaagg tcatccccct gttcgtgacc aatctgatcg acggaaagaa
    24121 ggtcccgctc tacggcgacg ggatgaacgt gcgcgactgg ctgcatgtcg atgaccactg
    24181 ccgcggcatc cagctggtgg ccgagaaggg tcgcgacggc gaaatctaca acatcggcgg
    24241 cggcacggag ctcacgaacc gcgagctgac gcaccggctg atcgacgcgg tgggcgctga
    24301 cgcctcgatg atcgtgtctg tccccgaccg taagggccac gaccggcgct actgcgtgga
    24361 ctggtcgaag atcgccgacg agctcggata cgccccgcgg gttccgttcg acgagggcct
    24421 ggccgcgacg gttcggtggt acgccgacaa ccggtcatgg tgggagccgc tcaagaaagg
    24481 gcagcgctga cgtagtccgc cagccggtcg cgccaattcg gcagcgccac accggcccgc
    24541 tccgccctgg tcaggtccag gacgctgttg agcggtcgcc gggcagcctg cggcttgtcg
    24601 gcgaagtagg cctcggtgga ggtctcggtg atggccagat cgccgtgccc ggacagggcg
    24661 taggtcgccc tcgcgacctc ggcccacgat gccggttccc cgctgcccgt cacgtggtag
    24721 gtcccgtacg gggcaccctt cgagacgaga tgcacgatcg ctgcggcgat atcctgcgcg
    24781 aacgtcgggc gaccgatctg gtcgcccacc accgtcggct cgacgcccct ggcggccagg
    24841 gcgagcatgg tgcggacgaa attacggccc tcgcccatca cccaggtcgg acgcatcaga
    24901 tagtgacgct ccacggtgcc gacggcgagg tcgcccgccg ccttcgacgc gccgtatgcg
    24961 ctcagcgggg cgatcggagc gtcctcgtgg tagggctcgt cggctcggcc gtcgaagacg
    25021 tactcggtgg agaggtggac cagcgtcagg ttgtgctccg acgccacgtg cgcgagggag
    25081 gccggcccca cggcgttggc ctgccacgcc gcgacccgcc cgtccgtggt ctccgcgccg
    25141 tcgacgtcgg tgaacgcggc ggcgttgacg agggtgtcgt agctggacca gtcgatgtcc
    25201 gcgccggccg cttcggtgat gtcgagcccc ctccgggaca gcgcggtggc ctgcggcagc
    25261 gccgcggcca gtgcgcggcc gacctgcccg ccggctccga tgatcagtgc ccgcacttac
    25321 accctcatcg gcatgacgtc ggccagcggc ggatgcccgc ggtccttgtc cgagcggatg
    25381 gtcgcctcct cgtcgatcgg ccaatcgata cccagcgccg gatcgaacgc ctgcacgagg
    25441 gtgtacgagg catccggcga ccagtgctca ttgacgaggt aggtgtagac gacgtcgggt
    25501 gtgagggtct ggtaagagtt gccgcacccc cggggcacga agatcgaatt gcctgggtgc
    25561 agctcgaatg tctccgatcg gccgaacgtg ggtccggctc gcaggtcaac gatcgcggcg
    25621 aacgctcggc cgtgcgcaag cgagatgtac ttgtcccacg gctcggcgtg tataccgcgg
    25681 gtgacgccga cctcggcatt gaaggagacg ttgttctgca cgacctcgaa gggcggaaaa
    25741 ccctgcgcct cgagcttcgc gcgctggaac ccttccttga accagccgcg cccgtcctgg
    25801 tgcaccacga ggtcgatgcg cagcagaccc ggtattgacg tctcgtccac acggagcgtc
    25861 ccgctcatat cgcccgtcct cgaggaggca cgtcggtcgg ctccccgggc gacatcgtgc
    25921 ggacccagcc gagcgtcgcc cgcagtccgt ccatgaggtc ggtcgacgcg gtgaggccga
    25981 gctcgtcacg cgcacgcgat acgtccagac agctgcgtcg cacctcgccc gcccgggccg
    26041 gccggaactc ggggacgaac gtggtgggtt ccacccccgc tgcctccgct acggccgcca
    26101 ccagggtgag tacgtcgatc tccttgcccg tgccgacgtt gtacgtgtcg tgcgacaggt
    26161 cggtggccgc caccaccgcc cggttcgccg ccacgatgtc gccgacgaac acgtagtcgc
    26221 gggtctggct gccgtctccg aacaccctgg gtcgactgcc ggagagcacg tggtcgcaga
    26281 agatcgcgac gacacccgcg tcaccccggg ggtcctggcg cgggccatag acgttcccgt
    26341 accgcagggt cacgacatcg agaccgtggc tgcgccggaa ccatcgaccg tagcgctcgg
    26401 ctgtctgctt gctcaggccg taggccgaga tcggatcagc gggcgtggtc tccggcgtgg
    26461 ggacgaggcc ggtctctccg tagatggcgc cacctgtcga ggtattgacc acgcggcggg
    26521 ttcctgcggc aagcgctgca gcgaagacgt tcaccgagcc gagcacgttg accgccgcgt
    26581 ccacagccgg ctcgagcatc gaggtcctga cgtcgatctg ggctgcgagg tgaaacacca
    26641 catcgggtcg gaacgcgagc atggcgcggt gcaccccggc gccgtcacga acgtcgagct
    26701 cggcgagttc ggctccccgg cctaccgcgt cggacaggtt gagtagggac ccgtggctga
    26761 aatcgtcgat gacgagaacg cctgcaccgt ctgcgatcag ggcgtcgacc aggttcgatc
    26821 cgacgaagcc cgctccaccg gtcactgccg ctcgcactgc gaaatcctag cgccggaccg
    26881 gtgccggccg acgtcgctct gccgaaccga attctgaggc gcgacccggg tgcgggaatg
    26941 gtggttcggc gggtttgccg acccacgagt gtgacgacac actgaccgtc gtgaccgctc
    27001 cagagggtcg gccgcgcacc gacgcgcccg actacgccga acgtctgcag aggcttgagc
    27061 aggcccattg gaagaaatgg ctcgacgtgc aggcgccgta ccggtggaac gttcggcgcc
    27121 tggacctcgg ccgcgtgctg gacgtcggtt gcggtatcgg tcggaatctg atgcacctcg
    27181 gtggttccgg agtgggcgtc gaccacaatg cggcgtccgt cgccgtggcg cgcgagcgcg
    27241 gcctcgaggc ctacacatcc gaggagttcc tcggcggccc gcaggcgcgg aagggttcgt
    27301 tcgacagcct gctcgccgcg catgtcgtcg agcacatgtc cgagccggac gcagttcgtg
    27361 tgctgcagac ctacctg
//
LOCUS       DASRSC010000025         3495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1480933,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000025 DASRSC010000000
VERSION     DASRSC010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1480933"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VFE39_01125"
     CDS             complement(<1..570)
                     /locus_tag="VFE39_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET6257228.1"
                     /translation="MTTIPLDPDELLTTTRSVRKRLDLDRPVPIELVRECLEVALQAP
                     TGSNQQGWHWLVVTDAGLRAKIGEYYRGSFEAYRASPGYGGHAGQSAERQAVQSRVSG
                     SAEYLAERMGDVPVHVIACITAAEELPAGNQAGLWGSLLPAAWSFQIAARARGLGSAW
                     TTLHLRYEKEIAELLGIPPNVRQGVLLPTA"
     gene            complement(604..795)
                     /locus_tag="VFE39_01130"
     CDS             complement(604..795)
                     /locus_tag="VFE39_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257229.1"
                     /translation="MDRSGVDAARTAQACPRTQELLDSVRAPLTAQRIWDNLTGAWER
                     TSYRYPRDPRPAATCGTAG"
     gene            complement(776..1324)
                     /locus_tag="VFE39_01135"
     CDS             complement(776..1324)
                     /locus_tag="VFE39_01135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257230.1"
                     /translation="MERRAVRRDAGRLEAVRADEVGRAWPVAFVLGLAPLVRPDLALF
                     GLLVAVALVVLEARRGWLRLVGLAVVAAAVPLAYEVFRAGYYGLLVPSTALAKEASTA
                     RWRQGGYYLLDLVTPYLLWIPVSVLLVGVLALLGGRRRTGVKVSRRACLATVAVVAVP
                     VHPLDADRLRHAGPELDGPKRR"
     gene            1669..>3495
                     /locus_tag="VFE39_01140"
     CDS             1669..>3495
                     /locus_tag="VFE39_01140"
                     /inference="COORDINATES: protein motif:HMM:NF016484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052636 - arabinosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071766 - Actinobacterium-type cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinosyltransferase domain-containing protein"
                     /protein_id="HET6257231.1"
                     /translation="MTDVSDAVRDNAAAPDGEADDRPVRPPGPPPPIGGRGRPTALLA
                     GLTALVAVVCGALLPFAPVSVSEPTVSWPLDPAHPESTLLSLTAYRPLILDVRFTCDA
                     GRAAQANGGVVVSTAAPEPPAPGTTGLIVTVRGDRVQIGALDRLLLDEPLPVGACEYR
                     ITGDSRGLPSFVRPASDPADPDAPDLASFAQPDNAELTISRDGTELVDTTALQLPDVD
                     VLATDATDLPSGGLIVRMRVDDEFTSSPTRLKSVLIWTLVTAVLATALLLLRLDRMTP
                     RVPHMWRVGWPRIVDVLVPAVIVFWMFVAPATDDDGYYAAIARNSVLSGDVGNYFQLY
                     DQNFTPFTWFYQSLGWWQQLAGNAPVMQRIPAVVLGILTWLALRRFAVAAMDEWAPHD
                     RGVRMTAHAALAVTFLSWWVPQNMGVRPETVVAVCGSATMLAVLAAGRRRRLGLAWLA
                     CALAGLGFTAHPTGFTLFAPLLAGLPLLLPVMRVAGDRAGTALRALAVASGGMVAPLL
                     AFADAGLRDFLRGQAIVLSLQDQESWTTEIQRYAFLFSQSPMGNYAKRAVVLVCLVAL
                     VWFAVIAVAARAREVAVPTPLWLAGSTTALAFAALWLIPSKWT"
BASE COUNT          467 a         1278 c         1223 g          527 t
ORIGIN      
        1 cgcggtgggc aggagtacgc cctgccggac gttcgggggg atcccgagca gctcggcgat
       61 ctccttctcg taacgcaggt gcagggtcgt ccaggcgctg ccgagtccgc gggcgcgagc
      121 ggcgatctgg aagctccacg cagccggcag cagggaaccc cacagccccg cctggttgcc
      181 cgccggcagc tcctcggcgg ccgtgatgca cgcgatcacg tgtacgggga catcacccat
      241 tcgttccgcg aggtactccg ccgagccgga gacccgggac tggacggcct gccgctccgc
      301 gctctggccg gcatgtcccc cgtaacccgg tgaggcacgg taggcctcga acgacccccg
      361 gtagtactcc ccgatcttcg cgcgcagtcc ggcatcggtc accaccaacc agtgccagcc
      421 ctgctggttg ctgccggtcg gtgcctgcag cgccacctcc aggcactcac gcacgagctc
      481 gatcggcacc ggccgatcga ggtcgagccg cttgcgcacc gagcgggtgg tggtgagcag
      541 ctcatcagga tcgaggggga tggttgtcat accgcaatca ttcccctaac ggagccgggg
      601 cgatcagccg gccgtgccgc aggtagcggc cgggcgcggg tcgcgcgggt accggtagga
      661 ggtgcgctcc catgcccccg tcaggttgtc ccagatccgc tgcgcggtga gcggtgctcg
      721 tacggagtcg agcagctcct gggttcgcgg gcaggcctgt gccgtgcggg ctgcgtcaac
      781 gccgcttcgg tccatccagt tcgggcccag cgtgtcgtag gcggtccgca tcgagaggat
      841 gcacgggcac ggcaaccacc gcgacagtgg cgaggcaggc ccgtcgggac accttcaccc
      901 cggtgcgcct gcgcccgccc agcagtgcga gcaccccgac gagcaggacc gacaccggga
      961 tccacagcag gtacggcgtc accaggtcga gcaggtagta cccgccctgc cgccagcggg
     1021 ccgtgctcgc ctccttggcc agcgcggtgg agggcaccag caggccgtag tagccggccc
     1081 ggaacacctc ataggccagc ggcaccgcag cggccaccac tgcgaggccg accaggcgaa
     1141 gccacccccg acgggcctcc aggactacca gcgcgacagc cacgagcagc ccgaagagcg
     1201 ccaggtcggg gcggacgagc ggggcgaggc cgaggacgaa cgcgaccggc cacgcgcggc
     1261 cgacctcgtc ggcgcggacg gcttcgagtc gaccagcgtc acggcgaaca gcgcgacgtt
     1321 ccatggggag cgggacgagg gagtcgacct gcgagccggc tcggcaaccg ggtacgcctc
     1381 cacgacagtg ctgatcggac ttctccctcg tggtcgtggc gcccgacacc cgccgagcgg
     1441 gtacatcgtg ccgggcagca ccggaggcct gctatttggt ccggctcccg ccggccgacg
     1501 agcgggcctt ccccgccaca tccggcatga cgatcagtgg cgagcggggc gtccgcgggc
     1561 cgcagcagca ccgccccctg gcaccgcaca cgttcctgtc atcccgtcgt gtgaacgtgg
     1621 ctcctctgca atcgttctca tggcgcctct ccgacagcat gaccgattgt gactgacgtg
     1681 tcggacgccg tgcgggacaa cgccgcggcg cctgacggag aggcggatga ccgccccgtc
     1741 cgcccgcccg gtccgccgcc gcccatcggt gggcggggtc gccccaccgc cctgctcgcc
     1801 gggctcaccg cgctggtggc agtcgtgtgc ggggctctgc tgccgttcgc tccggtctcg
     1861 gtctcggagc ccacggtgag ctggccgctg gacccggcgc acccggagtc gaccctgctc
     1921 tccctcacgg cctaccggcc gttgatcctc gacgtacgct tcacctgcga cgccggccgc
     1981 gccgcgcagg cgaatggcgg ggtcgtggtg tcgaccgcgg cgccggagcc accggcgccc
     2041 ggcaccaccg gcttgatcgt caccgtccgc ggtgaccgcg tgcagatcgg cgcgctggat
     2101 cggctgctgc tcgacgaacc gctgcccgtc ggcgcgtgcg agtatcggat cacgggcgac
     2161 agccggggcc tcccgagttt cgtccgacct gcgtccgacc ctgccgatcc ggatgcgccc
     2221 gacctcgcct cgttcgccca gccggacaac gccgagctga ccatcagccg cgacggcacg
     2281 gagctcgtgg acaccactgc gctgcaactc cccgacgtcg acgtgctggc caccgacgcg
     2341 acggacctcc cgtccggcgg gctgatcgtc cggatgcggg tggacgacga gttcaccagc
     2401 agccccaccc ggctcaagtc cgtgctgatc tggacgctgg tgacggccgt actcgccacc
     2461 gccctgctgc tgctgcgcct ggaccggatg acgccgaggg tgccgcacat gtggcgggtg
     2521 ggatggccgc ggatcgtgga cgtcctggtt cccgcggtga tcgtcttctg gatgttcgtg
     2581 gctcccgcga ccgacgacga cggctactac gcggcgatcg cgcgcaactc ggtgctctcc
     2641 ggcgacgtcg gcaactactt ccagctctac gaccagaact tcaccccgtt cacgtggttc
     2701 taccagtccc tgggctggtg gcagcagctc gcgggcaatg cgccggtgat gcagcggatc
     2761 cccgccgtgg tactcgggat cctcacctgg ctggcactgc gccggttcgc cgtggccgcg
     2821 atggacgagt gggctccgca cgaccgcggc gtgcggatga ccgcgcacgc cgcgctcgcg
     2881 gtgacgttcc tgtcgtggtg ggtgccgcag aacatgggcg tgcgacccga gacggttgtg
     2941 gcggtgtgtg gctcggccac gatgctcgcc gttctggctg cgggccgcag gcgccggctc
     3001 gggctcgcgt ggctcgcctg cgcgctcgcc gggctcggct tcacggcgca tcccacgggc
     3061 ttcacgctgt tcgcaccgct gctcgccggg ctgccgctgc tcttgcccgt catgcgggtg
     3121 gccggcgacc gcgcgggcac ggcgctgcgc gcgctggccg tcgcgtcggg cggcatggtc
     3181 gcaccgctgc tggccttcgc cgacgctggt ctgcgcgact tcctgcgcgg acaggcgatc
     3241 gtcctttccc tgcaggacca ggagagctgg accaccgaga tccagcgcta cgcgttcttg
     3301 ttctcgcaga gcccgatggg caactacgcg aaacgggccg tggtgctggt gtgcctggtc
     3361 gcgctcgtct ggttcgcggt gatcgcggtc gcagcacgcg cccgtgaggt cgcggtgccg
     3421 acaccgttgt ggctggccgg ctcgaccacc gcgcttgcgt tcgccgcgct ctggctcatc
     3481 ccgtccaaat ggacc
//
LOCUS       DASRSC010000026         7036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_498391,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000026 DASRSC010000000
VERSION     DASRSC010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7036
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498391"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VFE39_01145"
     CDS             <1..1028
                     /locus_tag="VFE39_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HET6257232.1"
                     /translation="VLFDELNMPKTKRTKTGYTTDADALQTLYEQTGHEFLSHLLLHR
                     DATRLKVTVDGLLKSVADDGRIHTTYSQTIAATGRLSSTEPNLQNVPIRTAAGRRIRE
                     TFVVGPGYSELMTADYSQIEMRIMAHLSEDTALIEAFRSQHDFHAETAARVFGCEPTE
                     VSPEQRAKIKAMNYGLAYGLSAFGLAAQLRISTEEARGLMDEYFKGFGGVRDYLRSVV
                     DIARKDGFTATIMGRRRYLPDLTSDNRQRREMAERMALNAPIQGSAADIIKVAMLGVH
                     RALTGEGLRSRMLLQVHDELVLEVAEGERATVETLVRREMAAAAQLSVPLEVSVGYGH
                     SWDDAAH"
     gene            1177..1746
                     /locus_tag="VFE39_01150"
     CDS             1177..1746
                     /locus_tag="VFE39_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6257233.1"
                     /translation="MQLLGSDDPLPHRPRRMLVAGCSGSGKSTVAAALAALLGVPHRE
                     LDALHHGPAWVPRPEFAADVAAFAATEEWVAEWQYSTVRPLLLARADLLVWLDLPRWR
                     VFDQLLRRTLRRRLHRVELWNGNIEPPLWTVFTDRDHVLRWAWQAYPRSRQRMRAVLE
                     ADSPVVVVVRLRTRRDVHAWLAGPVTAVR"
     gene            complement(1837..2424)
                     /locus_tag="VFE39_01155"
     CDS             complement(1837..2424)
                     /locus_tag="VFE39_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6257234.1"
                     /translation="MGDDRILLREFLRAPTRVATVTASSDALVAAMIAPLAIGDETVV
                     VELGAGTGRVTEALQRRLGGRGRHVAIEINAVLAERLATRHPAVTVVCADAAELPVVL
                     RTHGVAKADAISSLLPWVAYAGAPIPAIAAAALAPTGTFTQASLWLTHLLPPARRQER
                     DLRAAFGDVSVSPVVWRNLPPARVLAARSPAPLSV"
     gene            complement(2585..3076)
                     /locus_tag="VFE39_01160"
     CDS             complement(2585..3076)
                     /locus_tag="VFE39_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257235.1"
                     /translation="MSATDRLGIYLNDHLAGASAGVEMARRVQEAVQGDPAAEVLGPI
                     ATEIEEDLETLRRLVEVLGIGKNPVKQAAGWLTEKVHRLGVDERLTRSPDLTLLLQAE
                     SLSLGIEGKLALWLALMEVVSAHPQLSAVDLPAMAERARNQRRRVEAVRLGAARRAFA
                     TVD"
     gene            3243..3848
                     /gene="ruvC"
                     /locus_tag="VFE39_01165"
     CDS             3243..3848
                     /gene="ruvC"
                     /locus_tag="VFE39_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HET6257236.1"
                     /translation="MRVLGVDPGLTRCGLGVVDGAGGRTVRCVDVDVVRTPADAPVAE
                     RLLAVGAVVERWIERHRPDVVAIERVFSQHNVRTVMGTAQASGVVALLAARAGLPVAF
                     HTPSEVKAAVTGEGRAGKQQVTTMVTRLLGLDETPRPADAADALALAICHCWRAPMLD
                     RMAQAQARSAELARAHRARLAAAAKVAAGAKAAARAAEEAS"
     gene            3845..4447
                     /gene="ruvA"
                     /locus_tag="VFE39_01170"
     CDS             3845..4447
                     /gene="ruvA"
                     /locus_tag="VFE39_01170"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET6257237.1"
                     /translation="MISSVRGEVLSIGLDHAVVEVGGVGLAVHATPTTLAGLRRGESS
                     RLATALVVREDSLTLFGFAHDEERELFGLLQTVSGIGPRTALATLAVLTPDVLRRALV
                     DGDLTTITRVPGIGRKGAERLVLELRDKVDAPSAAPATLPVTPNGANPRREQVVEALV
                     GLGFTTKPAEQAVDAVLAGDGDGDASTLLRAALTRLGKSR"
     gene            4557..5588
                     /gene="ruvB"
                     /locus_tag="VFE39_01175"
     CDS             4557..5588
                     /gene="ruvB"
                     /locus_tag="VFE39_01175"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET6257238.1"
                     /translation="MSDVALTPEPEPDDLEVDATLRPRNLTEFIGQPRVREQLELVLE
                     GARRRGDPPDHILLSGPPGLGKTSLALIVAAEMGAAIRLTSGPALERAGDLAAMLSNL
                     EPGDVMFIDEIHRIARPAEEMLYLAMEDFRVDVVVGKGPGATSIPLDIAPFTLVGATT
                     RSGALTGPLRDRFGFTAHMEFYEPAELELVLRRAAVILDVDLRRDGGEEIASRSRGTP
                     RIANRLLRRVRDFAQVRADGAVTRQVARDALAVYDVDELGLDRLDRAVLGALVRSFGG
                     GPVGVSTLAVAVGEEPGTVEEVCEPYLVRAGMLARTPRGRVATAAAWSHLGIAPPPDV
                     TRGGPAALF"
     gene            5640..6068
                     /gene="yajC"
                     /locus_tag="VFE39_01180"
     CDS             5640..6068
                     /gene="yajC"
                     /locus_tag="VFE39_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET6257239.1"
                     /translation="MDLSLLFPLLILLLFIPIFLSGRRQRRQVAETQKLQAALDAGDV
                     VITTSGLRATVVDASYEDTLDLEIADGVVTTWLRAAIREKVNPVVDEAIVVDEAETPA
                     TAPVEPVEPVDKAPSLDKAPSLDKAPSLEKDDSANGSSRN"
     gene            6104..>7036
                     /locus_tag="VFE39_01185"
     CDS             6104..>7036
                     /locus_tag="VFE39_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HET6257240.1"
                     /translation="MATTPGRIRPWRYLAAFAGVVIVLYALVFFTGDGRAAPKLGIDL
                     QGGTRVTLQARTETGAVPPQEQLVQAQQIIEERVNGLGVSGAEVVLDGSNLTITVPGQ
                     EGDQARSLGQTAQLRFRQVIGGPVAAAPAAPPAGAPGAPAPTPGAPGAAPGPAAPPAA
                     PPSPAQQPQGAADPAAPVERGVAPVAYTTPLQNPTPPPTPPAPPAAPAPGSATPAGPA
                     DPQLAAAINAARQTRQSTDPAVQQMALATLDCKAQDPLRGYDDPTLPLVTCSQDGTEK
                     YVLGPSFLEGTQIANAVAQQNTQGAGWVISLTFKS"
BASE COUNT          992 a         2485 c         2601 g          958 t
ORIGIN      
        1 ccgtgctgtt cgacgagctc aacatgccga agaccaagcg caccaagacc ggctacacca
       61 ccgacgccga cgcgctgcag acgctgtacg agcagacggg gcacgagttc ctctcccacc
      121 tgctgctgca ccgcgacgcc acccggctca aggtcacggt cgacgggctg ctcaagtccg
      181 tggccgacga cgggcggatc cacaccacct acagccagac gatcgcggcc accgggcggc
      241 tgagctccac ggagcccaac ctgcagaacg tgccgatccg cacggcggcg ggccggcgga
      301 tccgcgagac gttcgtggtg ggcccggggt actccgagct gatgacggcc gactacagcc
      361 agatcgagat gcggatcatg gcgcacctgt cggaggacac cgcgctgatc gaggcgttcc
      421 ggtcgcagca cgatttccac gccgagaccg ccgcgcgggt cttcggctgc gagcccacgg
      481 aagtgtcgcc ggagcagcgc gccaagatca aggcgatgaa ctacggcctg gcctacgggc
      541 tctcggcgtt cgggctcgcc gcgcagctgc ggatctccac cgaggaggcc cgcggactga
      601 tggacgagta cttcaagggc ttcggtggag tgcgtgacta cctgcgcagc gtcgtcgaca
      661 tcgcccgcaa ggacgggttc accgccacga tcatgggtcg ccgccgctac ctgcccgacc
      721 tcaccagcga caaccgccag cgccgcgaga tggccgagcg gatggcgctc aacgcaccca
      781 tccagggcag cgccgccgac atcatcaagg tcgccatgct cggtgtgcac cgcgcgctca
      841 ccggggaggg cctgcgcagc cggatgctgc tgcaggtgca tgacgagctg gtgctcgagg
      901 tggccgaggg cgagcgggcc accgtggaga cgctcgtgcg ccgcgagatg gccgcggcgg
      961 cgcagctgag cgtgccgctg gaggtctcgg tcggctacgg ccacagctgg gacgacgcgg
     1021 cccactaggg ggacgaacgg cactttcgca cgtgaggtgt gtggacggga gtgctgttcg
     1081 tccgctcgac aacctgcccg acgatctcca ccggcaccgt cgctgccgtg accaccccca
     1141 tcggcccgac gccaccggcg ggtcgacgat ggccggatgc agctactcgg gtcggacgat
     1201 ccgttgccac accggccgcg acggatgctc gtcgcggggt gctccgggtc gggcaagagc
     1261 acggtggccg cggcgctggc ggcgctgctc ggcgtgcccc accgggagct cgacgcgttg
     1321 caccacggcc cggcgtgggt gccgcgtcct gagttcgccg cggacgtcgc ggccttcgcg
     1381 gccacggagg agtgggtggc ggagtggcag tacagcacgg tccggccgct actcctggcg
     1441 cgggcggacc tgctcgtctg gttggacctt ccgcgttggc gggtgttcga ccagctcctg
     1501 cggcgcacgc tgcggcggcg gctgcaccgc gtcgagttgt ggaacggaaa catcgaaccg
     1561 cccttgtgga cggtgttcac ggatcgcgat catgtcctgc gatgggcgtg gcaggcgtac
     1621 ccgcgcagtc gacagcggat gcgggcggta ctggaggcgg actccccggt ggtggtggtg
     1681 gtgcgcctgc gcacccggcg cgacgtccac gcctggcttg ccggaccggt gaccgccgtg
     1741 cggtagcgac aacccactgg gccgctctgc gggccctggt cctcgcagac ccggtggacc
     1801 ctgcgatgac ccgaacgggc gcgcggagcc gccggctcag acgctcaacg gagcggggga
     1861 gcgggccgcg aggacgcggg ccgggggcag gttgcgccac accaccgggc tgacggagac
     1921 gtcgccgaag gccgcgcgga ggtcgcgttc ctggcgacgg gccgggggca ggagatgggt
     1981 cagccagagc gaggcctgag tgaacgtgcc ggtgggagcc agtgccgcag ccgcgatggc
     2041 cgggatgggt gccccggcgt aggccaccca gggcagcagg ctggagatcg cgtcggcctt
     2101 cgcgaccccg tgcgtccgca ggactaccgg gagctcggcg gcgtcggcgc acacgaccgt
     2161 gacggcgggg tggcgcgtgg cgagccgttc ggccagtacg gcgttgatct cgatggcgac
     2221 gtgccggccg cggccgccca gccggcgttg cagggcttcg gtgacccggc cggtcccggc
     2281 cccgagctcg acgaccacgg tctcgtcccc gatcgccagc ggcgcgatca tcgcggcgac
     2341 gagcgcatcg gagctcgccg tgacggtggc gacacgcgtc ggtgcacgga ggaactcacg
     2401 cagcaggatc cggtcgtcgc ccaccgtcgg aggatagggc ggccggcccc gcatgaaccc
     2461 ggaccgacga gccggcgcct accgcgcgac catgcggttg ccgtgcggtg acgggtgcac
     2521 gagaagatgc cgtgcggaac ggtgcggact ccggctgacc ggcgtgggag cgatgcggcc
     2581 gggattagtc gacggtggcg aatgccctgc gggccgcgcc cagccgcaca gcctccaccc
     2641 tgcggcgctg gttccgcgcg cgctcggcca tggcgggcag gtcgaccgcg ctgagctgtg
     2701 ggtgggcgga caccacttcc atcagggcca gccagagcgc gagcttcccc tcgatgccga
     2761 gcgagaggct ctccgcctgc agcagcagcg tcaggtcggg actgcgggtc agccgctcgt
     2821 cgacgcccag ccggtgaacc ttctcggtga gccagcctgc ggcctgcttc accgggttct
     2881 tgccgatccc gagcacctcg acgaggcgtc gcagcgtctc caggtcctcc tcgatctccg
     2941 tggcgatagg cccgaggacc tcggctgctg gatcgccctg gacggcttcc tggacccgcc
     3001 gggccatctc gacaccggct gacgcacccg cgaggtggtc gttgaggtag atgccgagac
     3061 ggtctgttgc ggacatgctg actcctcggt gcggactgcg cacagtgccg atggtgcatg
     3121 ggctgttggt gcacagggtc ctacggaagt ggcgtccccg ccgacggcgc gccccgtatg
     3181 gggccggata cggcgccggt agcctgccct ctcgaacggc tgttcgcgcg agaggagacg
     3241 gtgtgcgggt gctcggcgtc gacccgggac tgacccggtg cgggctcggc gtcgtcgacg
     3301 gtgccggtgg gcgcaccgtg cggtgcgtgg acgtcgacgt cgtgcgtacc cccgcggacg
     3361 cgccggttgc ggagcggctc ctggccgtcg gtgccgtcgt cgagcggtgg atcgagcggc
     3421 accggcccga cgtcgtggcg atcgaacggg tcttcagcca gcacaacgtc cggacggtga
     3481 tgggcacggc gcaggccagc ggggtggtcg cgctgctcgc ggcgcgggcc gggctgcccg
     3541 tcgcgttcca cacgcccagc gaggtgaaag cggccgtcac gggtgagggc cgggcgggca
     3601 agcagcaggt caccaccatg gtcacccgcc tgctcgggct cgacgagaca ccccggcccg
     3661 ccgacgcggc cgacgcgctg gccctcgcga tctgccactg ttggcgggca cccatgctgg
     3721 atcggatggc acaggcgcag gcccggtcgg cggagctggc gcgtgcccac agggcgcggc
     3781 tggccgcggc ggcgaaggtg gcggcaggag cgaaggcggc ggcgcgggcc gcggaggagg
     3841 cgtcgtgatc agttcggtgc gcggtgaggt gctgtcgatc gggctggacc acgccgtcgt
     3901 cgaggtgggc ggcgtgggcc tggccgtcca tgccacgccc accacgctgg ccgggctgcg
     3961 gcgcggcgag tcgagcaggc tcgccacggc gctcgtggtg cgggaggact cgctgaccct
     4021 gttcggcttc gcccacgacg aggagcgtga gctgttcggg ttgttgcaga cggtcagtgg
     4081 catcggcccg cgcacggcgc tggccaccct tgcggtgctc acacccgacg tactgcgccg
     4141 cgcgctcgtc gacggcgacc tgaccacaat cacccgggtg cccgggatcg gacgcaaggg
     4201 cgcggagcgc ctcgtgctgg agctgcggga caaggtcgac gcgccgtcgg ccgccccggc
     4261 cacgctcccg gtcacaccga acggcgcgaa tccgcgccgc gagcaggtcg tggaggccct
     4321 cgtcggcctg gggttcacga caaagcccgc cgagcaggcc gtggacgcgg tgctggccgg
     4381 cgacggcgac ggcgacgcga gcacgctcct gcgcgccgcc ctgacccgcc tgggcaaatc
     4441 ccgatgaccg cgacccgatc gtgggcccgg tccccgacat ggacacctgt tgctgttcga
     4501 tgcgcgtgcg gaacagcggt atgtgtccat gtcgcgcctg ggggtggggg cggcgcatga
     4561 gcgatgttgc gctcacgccg gagcccgagc ccgacgacct cgaggtcgac gcgaccctgc
     4621 ggccccgcaa cctcaccgag ttcatcggcc agccgcgggt ccgggagcag ctcgagctgg
     4681 ttctggaggg cgcgcggcgg cgcggtgacc cacccgacca catcctgtta tcgggtccgc
     4741 cggggctggg caagacgagc ctcgcgctca tcgtggccgc cgagatgggc gcagcgatcc
     4801 ggctgacgtc gggcccggcg ctggaacggg cgggcgacct cgccgcgatg ctgtccaacc
     4861 tcgagcctgg cgacgtgatg ttcatcgatg agatccaccg gatcgcccgc cccgccgagg
     4921 agatgctcta cctcgcgatg gaggacttcc gggtcgacgt ggtggtgggc aaggggccgg
     4981 gcgccacgtc catcccgctc gacatcgcgc cgttcacgct ggtcggcgcg acgacgcggt
     5041 ccggggcgct caccggaccg cttcgcgacc ggttcggttt caccgcgcac atggagttct
     5101 acgagcccgc tgagctggag ctcgtgctgc gccgcgccgc cgtcatcctc gacgtcgacc
     5161 tgcgccggga cggcggcgag gagatcgcgt cgcggtcccg ggggaccccg cgtatcgcga
     5221 accgcctgct gcgccgagta cgcgacttcg cccaggtgcg cgccgacggt gccgtcaccc
     5281 gtcaggtggc ccgcgacgcg ctggccgtct acgacgtcga cgagctcggg ctcgaccggc
     5341 tggaccgggc cgtgctgggg gcgctcgtgc ggtcgttcgg gggcggcccg gtgggcgttt
     5401 ccacgctcgc tgtcgcagtc ggcgaggaac ccggtaccgt cgaagaggtc tgcgagccgt
     5461 acctggtgcg ggccggaatg ctggcgcgca ccccccgggg ccgggtggcc acagcggcag
     5521 cctggtccca tctgggcatc gctccgccac cggacgttac ccgaggtggc cctgccgcgt
     5581 tgttctgagc gggggctggc agactcaggt ggctctcgac gtgcggagat acggagacca
     5641 tggacctctc gctgctgttc ccactgctca tcttgctact gttcatcccg atcttcctgt
     5701 cgggccggcg ccagcgccgt caggttgcgg aaacgcagaa gctgcaggcg gcactcgatg
     5761 ccggtgacgt ggtgatcacc acgtcgggcc tgcgtgccac ggtcgtcgac gcctcctacg
     5821 aggacaccct cgacctcgag atcgcggacg gcgtcgtcac cacatggttg cgggcagcca
     5881 tccgcgagaa ggtcaacccg gtcgtcgacg aggcgatcgt cgtcgacgag gcggagaccc
     5941 cggccaccgc cccggtggag cccgtggaac cagtggacaa ggcgccgtcg ttggacaagg
     6001 caccgtcgtt ggacaaggca ccgtcgctgg agaaggacga ctccgcgaac ggctcctccc
     6061 gcaactgacg cgggcgttac ctgacaggaa gaagatctca gcagtggcaa ccaccccggg
     6121 gcggatccgt ccctggcgct atctggcggc gttcgccggt gtcgtcatcg tgctctacgc
     6181 actggtgttc ttcaccggtg acggccgcgc cgcccccaaa ctgggcatcg acctgcaggg
     6241 cggcacccgt gtcaccctgc aggcgcgtac cgagaccggt gcggtcccgc cgcaggaaca
     6301 gctcgtgcag gcgcagcaga tcatcgagga acgggtcaac ggcctcggcg tcagcggcgc
     6361 ggaggtggtg ctcgacggga gcaacctgac gatcaccgtg cccgggcagg agggcgacca
     6421 ggcccgctcg ctggggcaga ccgcccagct gcggttccgc caggtgatcg gcggaccggt
     6481 ggcggctgct ccggccgcac cacccgccgg cgccccggga gcgccggctc ccaccccggg
     6541 cgcccccggt gccgctcccg gcccggctgc tcctcctgcc gctcctccgt ctccggccca
     6601 gcagccgcag ggcgcggccg acccggcggc tccggtggag cgcggggtgg ctccggtggc
     6661 ctacacgaca ccactgcaga accccacgcc cccgccgacg ccacccgcgc ccccggccgc
     6721 cccggccccg ggctcggcga ccccggccgg gccggccgac ccgcagctcg ccgccgcgat
     6781 caacgcggcc cggcagaccc gacagagcac tgatcccgcg gtgcagcaga tggcgctggc
     6841 gaccctcgac tgcaaagcgc aggacccgct gcgtggctac gacgacccca cgctcccact
     6901 ggtcacctgc agccaggacg gcaccgagaa gtacgtcctc ggaccgagct tcctcgaggg
     6961 cacacagatc gccaacgcgg tggcgcagca gaacacgcag ggcgccggct gggtcatcag
     7021 cctcacgttc aagagc
//
LOCUS       DASRSC010000027        12113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3119360,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000027 DASRSC010000000
VERSION     DASRSC010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3119360"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            446..739
                     /locus_tag="VFE39_01190"
     CDS             446..739
                     /locus_tag="VFE39_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET6257241.1"
                     /translation="MSDVSRLPGPMDHAWQWQRLGSCRGMDSAVFFHPDGERNPSRAR
                     RTAQAKAVCHRCPVIEQCREFALQTREPFGVWGGLAESERRVILEQRGVPLAS"
     gene            complement(803..1249)
                     /locus_tag="VFE39_01195"
     CDS             complement(803..1249)
                     /locus_tag="VFE39_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257242.1"
                     /translation="MELLRPESDLARLVEAFEHVDVLVVGDDEPVVCVVDVLNTHGDT
                     YTDGRHEVLRGEVARLGHSDLHLHRLGLRPPVGRGAEHDLVAAMSELVGFDPEPGVYC
                     LAPAPAPADPGRTAANRAAQRIAQVYGIPLLRYRCLELSVVGDAAP"
     gene            complement(1552..3768)
                     /locus_tag="VFE39_01200"
     CDS             complement(1552..3768)
                     /locus_tag="VFE39_01200"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET6257243.1"
                     /translation="MEDGGGTAGAPRVGTAANLPQVMDGAPVAILVIDLEGRQVVHAN
                     AAAVELTGDSVRLPVDIDDWGDAAGLTDLGGRRMSSTRSPLSLVASGVPVAGEPVAVH
                     DAARRGSGVTCVQRAAREGRLLWVTGFSLSGMADGVVPAGLAPGTNARALVALLPLSG
                     TEHADRRRLEILRDRAVLATDMSFSITDPQQDDNPLIWVNPSFSKLTGYGYEEVVGRN
                     CRLLQGPNTDAAAVQRIHEALRRREPVTEVLVNYRRDGTAFWNQVAISPVFDGDGDLV
                     NFVGVQNDVTERVMVEQERRSALAEAEEARSQLRLLAEATTQMTGALDVTDACQRLAR
                     SLVPELADLCAVDLLERPGVGVPQRIAVAAREPADEARLRELGRLRNYRAGSGSDTGS
                     VLAGELPVLVPELPERGADRYPDDPAAAVVYDTLRLRSVMVVPVRARGRVLGALTLVT
                     QHPYGRRYGSRDLHLATDIAGRAGLTVDNARLYEVEHAAAVTLQRSLLPAVPEVAGVQ
                     VAARYLVGVDGNQVGGDWYDVLNLPDGALGLAVGDVVGHDLRAAAAMGQLRGVVRSYA
                     WDGGPPGSVLDRCDQLVQGLEMAAMATAVYARIEPLDHTGERLLRYANAGHPAPLLLG
                     PDGKLVRLDEHSSPMIGAVRSHGRPAGPGRTEAALRCRPGSTLLLYTDGLTDVAGEDA
                     DERTELLERTLAAMAPGSGPETVVEQVLAACLPQRLRDDVALLAVRLDDQPAAFER"
     gene            complement(3829..5232)
                     /locus_tag="VFE39_01205"
     CDS             complement(3829..5232)
                     /locus_tag="VFE39_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET6257244.1"
                     /translation="MSATPETSRTDPYTLTPDGIKDPPVGWRASFRYLGPGLILSASI
                     VGSGELIATTTLGAEVGFALLWLVIFSTFVKVAVQVELARWTIATGQPALTGYNRVPP
                     RIGRMGWVNVLWMLLAVTKLLQLGGIIGGTAVAFSILMPIGGDPLGYTSLLIWTVFLA
                     VGSIALLYSNSYTLIERGAVLLVVVFTFVTVLIALGLPLTPFSYGAGDIASGLTFLIP
                     AGALGAALAMFGITGVGADEITFYTYWCVEKGYARWAGPNDGSEAWVRRANGWIRVMY
                     KDAFISWVIYTFGTLAFYLMGAAVLNPQGLVPEGNGMITTLSRIYTDTLGDWAGIVFL
                     IGAIAVLGSTMWAAVPSWSRMYVNLLATMGVLDWQDPVARLRWIRIFTVALPILWAVA
                     YLTIQSPVVMVQIGGVMTGIFLVATVIAVWYLRRTETDPRLYGGSVFNTLLVVSSVAI
                     GLLGVYTLLQVFGVGIG"
     gene            complement(5716..7512)
                     /locus_tag="VFE39_01210"
     CDS             complement(5716..7512)
                     /locus_tag="VFE39_01210"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HET6257245.1"
                     /translation="MNADEPRRLRSQAWFDNPDNPGMTALYIERYMNWGITREELQSG
                     KPIIGIAQTGSDLAPCNRHHLQLADRVREGIREMGGIAFEFPVHPIQETGKRPTAALD
                     RNLAYLGLVEVLYGYPIDGVVLTTGCDKTTPACLMAAATVNIPAIVLSGGPMLNGWYN
                     GERTGSGTIVWKARELLAAGEIDQATFVEMVASSAPSPGHCNTMGTASTMNALAEALG
                     MSLPGCAAIPAPHRDRARIAYRTGRRIVEMVHEDLKPSDILTRQAFENAIVVNSAIGG
                     STNAPIHINAIARHIGVPLVMDDWERIGLTVPLIVDLQPAGKYLGEEFHRAGGVPAVV
                     HELIAHGLVHEDAPTVNGRTLGENCRENAPSDRDVIRTFDDALVGDAGFIVLSGNLFD
                     SAIMKTSVISPEFRERYLCDPEHPNVLQGRAVVFDGPEDYHARIDDPELDIDEECLLF
                     VRGAGPLGYPGAAEVVNMQPPAALIKRGVHALPCIGDGRQSGTSGSPSILNASPEAAA
                     GGGLALLRTGDMVRIDLGKGTADILLPADELVERRRQLDDAGGYPIPPSQTPWQEIQR
                     SMVDQLSDGMVLRPAVEYQRVAQSKGVPRDNH"
     gene            complement(7535..8332)
                     /locus_tag="VFE39_01215"
     CDS             complement(7535..8332)
                     /locus_tag="VFE39_01215"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6257246.1"
                     /translation="MAARGGHVMGNEMLDFQEQVVLVTGASSGIGAAVAEGFARQGAR
                     VVVHYNRNSDGAEKVVTAIQEAGGTATSAAADLAQPAEAAGLVDDVVSRHGRLDVLVN
                     NAGDLLGRVPVTEMSDEDFRRVMDVNMTSVFAMSRQAVPVMRRQASGSIINVTSVAAR
                     TGGGGGSVAYATSKGAVSTFTRGLAKELAPEGIRVNAVAPGVITTPFHERNTTDAQMD
                     AMVSSVPMGRPGRPDECVGAVLFLASPAMSGYITGQVLEINGGQLTP"
     gene            complement(8308..9843)
                     /locus_tag="VFE39_01220"
     CDS             complement(8308..9843)
                     /locus_tag="VFE39_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase family protein"
                     /protein_id="HET6257247.1"
                     /translation="MSVAPSFDEIGVRLHAADNVVVASRDLEEGTAAGGLTLRHAVPR
                     GHKVATAPIAAGEPVRKYNQIIGFAAVAIEPGDHVHTHNVEFKAFERDYAAGVDATVT
                     ELVPGGERATFEGIVRADGRVATRNYLGILTSVNCSATTAKLIADQFRRPGLLDDFPN
                     VDGVVALTHGAGCGMASEGEGFDVLQRTLTGYAEHPNFGGFLLLGLGCEVNQVASLAE
                     KWTLDATKAVRTMTIQEEGGTAATVRAGTAALLEMLPAANEVSRTTVPASELVLGLNC
                     GGSDAYSGITANPALGAAVDLLVRHGGTAVLGETPEIYGAEHLLTRRAVSQEVADRLV
                     ERIRWWEKYTADNNGSMDNNPSPGNKAGGLTTILEKSLGAVAKGGTTNLTAVYRYAEK
                     VTDRGFTFMDTPGYDPVSVTGIVAGGATVMCFTTGRGSVLGCKPTPSLKLATNTEMYE
                     RMSEDMDLNCGVIVDGSASVQEVGRELFQLILDTASGRRTRSEELGFGQEEFVPWQLG
                     AVM"
     gene            complement(9843..11180)
                     /locus_tag="VFE39_01225"
     CDS             complement(9843..11180)
                     /locus_tag="VFE39_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257248.1"
                     /translation="MTTKDLGPFEAVRGLRFDAPFPRLLPVRQDFDTTRIDDVAAATV
                     AALEPLRSRVAPGMSVAITVGSRGIHDKREVVRAAGEWLRSVGAEPFVVPAMGSHGGA
                     TADGQVQLLANLGMTEESLGMPIHATMETVELERVPGGPAVHLDAHAAKADAILAVNR
                     VKAHTDFSGEVESGLAKIVAIGLGKQRGAEGIHRFGPANLGVWVPQVARRIVQTGKVL
                     GGLAIIENAGDRAARIELVGAEDIGGPVETALLADAKRRMATLPFDDIDVAVVDVMGK
                     NFSGSGMDTNVIGRMMIRGSAEFERPRITNIAVLDVSDASHGNAVGLGLADFVPFRLL
                     EKLDLQAVYINAMTSGLGGPQRAQVPMAMPTDRSTVAAAILTCGRPDLERVTLVRMRN
                     TLDLEQLWVSESLRERVLADPKLEITGDAVPMTFDDHGHLLPWAYAPTTTGES"
     gene            complement(11177..>12113)
                     /gene="pdxA"
                     /locus_tag="VFE39_01230"
     CDS             complement(11177..>12113)
                     /gene="pdxA"
                     /locus_tag="VFE39_01230"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HET6257249.1"
                     /translation="DPRVDVARLVAIGSLRALRSAAAVVPDAPALREVSAPGEARFEP
                     GTLDVLTVPSDVAVPIGKVSAEAGQIAFEGVERAVALALAGEIDGIVTAPLNKEALAL
                     AGHPYPGHTEILADLTGTTDFAMMLTALKLRVVHVTTHVGIRQMLDRVTTERVLTTIR
                     LAHRALRDMGIDEPSVGVAGLNPHAGEGGLFGDEEARVITPAIDRAKAEGIAASGPWP
                     ADTLFGRAVAGDFDVAIAMFHDQGHIAVKVLGFETGVNATIGLPIVRTSVDHGTAFDI
                     AGTGRAGADSLVEAVTVGAAMAQARAAARACGEPL"
BASE COUNT         1756 a         4348 c         4227 g         1782 t
ORIGIN      
        1 tcgccgtcct catcgaggtg cgcgaggctg cccacaaggc cggggtcgag ctgcgcctgg
       61 cgtgcaccgc ccgccgggtg ctccgcccgt tgaccatcgc cgggctcgtc ccgctcttcg
      121 atatccacga caccgtcgac caggcgatgg ccgccactta ggcgcacgga cagcaagcgc
      181 ctggtgtgat gtacatcaca gccgttcagg ctggtgagtg accgacaggc ggtccggacg
      241 tacggggagc ccccgaaagg ggctgtgagc tgctcgaacg gttgctcgcg ccgcctgtcg
      301 tcgatccgct gcgctgccgc gactcaacgg ccgtcgggct atcggcgctg cggcatgccc
      361 gattcttggc gtttggcccg tcaatcggca gggaacgagc acctcgatcg cccgatacgt
      421 cgggcacgaa cgacaagagg tgacgatgag cgacgtttcc cggctacccg gcccgatgga
      481 ccacgcctgg cagtggcagc gcctggggtc ctgtcgcggg atggacagcg ccgtgttctt
      541 ccacccggac ggtgaacgca atccttccag ggctcgccgg accgcgcagg ccaaagccgt
      601 ctgccaccgc tgccccgtca tcgagcagtg ccgggagttt gctctgcaga cgcgcgagcc
      661 gttcggtgtc tggggtggcc tcgccgagtc cgagcggcgc gtgatcctgg agcagcgggg
      721 agtaccgctc gccagctgag cgggcaagca cgaacggacg aatggggcgg tggcccgaac
      781 cgggccgccc ccttcgtcgt cttcagggcg cggcgtcccc gacgacggac agttcgaggc
      841 accgataacg cagcaacggg atgccataca cctgcgcaat gcgctgggcg gcccggttgg
      901 cggcggtacg accgggatcc gccggggcgg gggcaggcgc caagcagtag acgcccggct
      961 ccggatcgaa accgaccagc tcgctcatcg cggccaccag atcgtgctcg gcgccccgac
     1021 cgaccggcgg acgcagcccc aggcggtgca ggtggaggtc ggagtgaccg aggcgggcca
     1081 cctcaccgcg gaggacctcg tgccggccgt cggtgtacgt gtcgccatgg gtgttcagca
     1141 cgtcgacgac gcagaccacc ggctcgtcgt caccgacgac gagcacatcc acgtgctcga
     1201 acgcctcgac gagccgtgcg aggtcggatt cgggtcgcag cagctccatc ggtgtgacgg
     1261 tgacggcgac tgcgcgccgc cggtgcagga aaaccggcga tgaacctgtg acgggtgccg
     1321 ccaccggcgc ggagtacgac atctccggaa ccgcgaccat cgagaacgtc cccctagacg
     1381 catgccgacc cctcgccccc gaggcgcctc aactgcctgt tctgttgaga attgaaccgc
     1441 tacgcgagag tgggcgctca cgtcacgcga cgtcgaacgg caagcggcgc gccggggtcc
     1501 gccaacggcc tcctaccacc gggtaacagt gtggtggcgc tgggacgccg ctcagcgctc
     1561 gaacgccgcc ggttgatcgt cgaggcggac cgcgagcagc gccacgtcgt cgcggagacg
     1621 ctgcgggaga cacgccgcca gcacctgctc gaccaccgtc tcgggacccg agccgggtgc
     1681 catcgcggcc agggtgcgct ccaggagctc ggtgcgctcg tcggcatcct caccagcaac
     1741 gtcggtgagc ccgtcggtgt agagcagcag ggtcgagccg ggccgacagc gcagcgcggc
     1801 ctcggtgcgg ccagggcctg cgggccgccc gtggctccgg acggccccga tcatcggcga
     1861 gctgtgctcg tcgagccgca cgagcttgcc gtccggaccg agcagcaagg gcgcggggtg
     1921 cccagcattg gcgtaccgca ggagccgctc gcccgtgtgg tccagaggtt cgatccgtgc
     1981 gtacaccgcg gtggccatgg ccgccatctc caggccctgt acgagctggt cgcagcggtc
     2041 gagcaccgaa ccgggcggcc cgccgtccca cgcgtaggac cgcacgacac cgcgcagctg
     2101 ccccattgcg gccgcggccc tcaggtcgtg cccgaccacg tccccgacgg ccagtccgag
     2161 cgcaccgtcg ggcaggttca gcacgtcgta ccagtccccg cccacctggt tgccatcgac
     2221 gcccaccagg taacgcgccg ccacctgcac gccggcgacc tcgggcacgg ccggcaggag
     2281 gctccgctgc agcgtgaccg cggccgcgtg ctccacctcg tacagccgcg cgttgtcgac
     2341 cgtcagtccg gccctaccgg cgatgtcggt ggccagatgc aggtcccggg atccgtaccg
     2401 gcgtccgtag gggtgctggg tcacgagggt gagcgccccg agcacccggc cccgggcccg
     2461 gaccggcacg accatcaccg accgcagccg cagggtgtcg tacacgacgg ctgcagccgg
     2521 gtcgtcggga tagcggtcgg cgccgcgctc gggcagctcc ggcaccagga cgggaagctc
     2581 ccccgcgagc acactgccgg tgtcgctgcc gctgcccgca cggtagttgc gcagcctgcc
     2641 cagctcgcgc aggcgcgcct cgtcggcggg ttcccgtgcc gccacggcga tgcgctgcgg
     2701 caccccgacg cccggccgct ccagcaggtc gacggcacac aggtcggcca gctctgggac
     2761 caggctgcgg gcgagccgct ggcatgcgtc ggtcacatcc agcgcgccgg tcatctgggt
     2821 tgtcgcctcc gcgaggaggc gcagctggct gcgggcctcc tcggcctcgg ccagcgcgct
     2881 cctgcgctcc tgctcgacca tcacccgctc ggtgacgtcg ttctgcacgc ccacgaagtt
     2941 gaccaggtcg ccgtccccgt cgaacaccgg ggagatcgcc acctggttcc agaacgcggt
     3001 gccgtcgcgc cggtagttga ccagcacctc ggtgaccggc tcgcggcgcc ggagggcttc
     3061 gtgaatccgc tgcaccgccg ccgcgtcggt gttcggcccc tgcagcagcc ggcagttgcg
     3121 cccgaccacc tcctcgtacc cgtagcccgt gagcttcgag aacgacgggt tgacccagat
     3181 cagcgggttg tcgtcctgct gtgggtcggt gatggagaac gacatgtcgg tggcgaggac
     3241 ggcgcggtcc cgcagaatct ccagccgccg ccggtcggcg tgctcggtgc cggagagcgg
     3301 aagtaacgcg accagcgcac gggcgttcgt gcccggggcg aggccggccg gcaccacgcc
     3361 gtcggccatg ccggaaaggc tgaacccggt gacccagagc agcctgccct ccctggcggc
     3421 ccgctgtacg caggtgacgc cggagccacg ccgcgccgcg tcgtgcaccg ccaccggctc
     3481 ccccgccacc ggcacgccgc tcgccacgag cgacagcggc gagcgggtgc tgctcatccg
     3541 cctgccgccg aggtcggtga gcccggccgc gtccccccag tcgtcgatgt cgacgggcag
     3601 ccgcacactg tcgccggtga gctcgacggc cgccgcgttc gcgtggacga cctgccgacc
     3661 ctcgaggtcg atcaccagaa tcgcgacggg ggcgccgtcc atcacctgtg ggaggttcgc
     3721 ggcggtgccc accctcgggg cgcccgcagt ccctccgccg tcctccacgc gacccagcct
     3781 aagggcaggc cgtgcaacgt gcgccgtcat gacgggacgg tcaccgcctc agccgatccc
     3841 caccccgaag acctgcagca gcgtatagac gccgagcagc ccgatcgcga cgctgctgac
     3901 gacgagcagc gtgttgaaga cggacccgcc gtagagccgc ggatctgtct cggtgcgccg
     3961 gaggtaccag accgcgatga ccgtcgccac caggaagatg ccggtcatga cgccgccgat
     4021 ctgcaccatg accaccggcg actggatggt caggtaggcc acggcccaga ggatcggcag
     4081 agcgaccgtg aagatgcgga tccaccgcag ccgagccacg gggtcctgcc agtcgagcac
     4141 gcccatcgtc gccagcaggt tcacatacat ccgggaccag ctggggacgg cggcccacat
     4201 cgtcgaaccg agcaccgcga tggcaccgat caggaagacg atcccggccc agtcgccgag
     4261 ggtgtcggtg tagatgcgcg acagcgtggt gatcatcccg ttgccctcgg gcacgaggcc
     4321 ctgggggttc agcaccgccg cgcccatcag gtagaaggcg agcgtgccga acgtgtagat
     4381 gacccaggag atgaacgcgt ccttgtacat cacccggatc cagccgttcg cccggcgcac
     4441 ccaggcctcg ctgccgtcgt tcgggccggc ccagcgcgca tagcccttct cgacgcacca
     4501 gtacgtgtag aaggtgatct cgtcggcgcc gacgccggtg ataccgaaca tcgcgagcgc
     4561 cgcgccgaga gcacccgcgg ggatgaggaa cgtcagcccg ctcgcgatgt cgcccgcgcc
     4621 gtaggagaac ggtgtcagcg ggaggccgag ggcgatgagg accgtgacga aggtgaacac
     4681 caccacgagg agcaccgcgc cccgctcgat caacgtgtac gagttcgagt acaggagcgc
     4741 gatgctgccc acggcgagga acaccgtcca gatgagcagc gacgtgtagc cgagcggatc
     4801 gcccccgatc ggcatcagga tgctgaacgc cacggcagtg ccgccgatga tcccgcccaa
     4861 ctggagcagc ttggtcaccg ccagcagcat ccacaggacg ttgacccagc ccatccggcc
     4921 gatcctcggc ggcaccctgt tgtagccggt gagcgcgggc tgcccggtcg cgatggtcca
     4981 gcgcgcgagc tcgacctgca ccgcgacctt cacgaacgtg ctgaagatga ccagccagag
     5041 cagcgcgaag cccacctccg cgccgagcgt tgtggtggcg atgagctctc ccgaccccac
     5101 gatcgacgcg ctgaggatca gaccggggcc gaggtaccgg aaactggccc tccacccgac
     5161 cggtgggtcc ttgattccat cgggggtcag cgtgtagggg tctgtgcgag aggtctcggg
     5221 tgtggcggac atgctcggct cctttgccgc tggccatgtt cccggtcggt tctgccacgt
     5281 gcacgtgatg atggacattc gacacgaaac acgtcaatgc cccatcgctc gtcgcaggtc
     5341 agaaggcacg agggcgtcgt ggccggtgac gatcggcccc gtaccggctg cggaccaacc
     5401 gacatggacc gacccgggga gcgcctaccg tcgccgcgcg gtgtcgccgg ccggtggcgg
     5461 cccgctcgag ctcgtcgagt tcgaactgcc ccggggcacc gagctctcct acgccgccga
     5521 cacctacgcc ttcgtccacc agcagatctg gttctggacg gccgtctgct cttccacgag
     5581 ggcgacaccg agcacgagct cgaccggcga cagcctccag ctcggccccc ggcaccgtcc
     5641 tcgttcgtcg acccgaccgg tgcctgccgc tacctcgtcg cactcacgaa gcgcagctga
     5701 gcttggagcg cgagctcagt ggttgtcgcg cggcaccccc ttgctctggg ccacgcgctg
     5761 gtactcgacg gcgggccgga gcaccatgcc gtcggacagc tggtcgacca tcgagcgctg
     5821 gatctcctgc cagggggtct gcgacggcgg gatcgggtag ccgccggcgt cgtcgagctg
     5881 tcggcgacgc tccaccagct cgtcggccgg gaggaggatg tcggcggtcc ccttgccgag
     5941 gtcgatgcgg accatgtccc cggtacgcag cagcgccagc ccgccgcctg cggcagcctc
     6001 gggcgaggcg ttcaggatcg acggcgatcc ggacgtgccc gactgccggc cgtcaccgat
     6061 acacgggagc gcgtgcacgc ccctcttgat cagcgctgcc ggcggctgca tgttgacgac
     6121 ctcggccgcg ccggggtacc cgagtggccc ggcgccccgg acgaacagca ggcactcctc
     6181 gtcgatgtcg agctccgggt cgtcgatgcg ggcgtggtag tcctccggcc cgtcgaagac
     6241 cacggcacgg ccctgcagga cgttcgggtg ctccgggtcg cacaggtagc gctcgcggaa
     6301 ctccggggag atcacgctgg tcttcatgat ggcgctgtcg aacaggttgc cgctcagcac
     6361 gatgaagccc gcgtccccca ccagcgcatc gtcgaacgtg cggatgacgt cacggtcgga
     6421 cggggcgttc tcccggcagt tctcacccag ggtccggccg ttcacggtcg gcgcgtcctc
     6481 gtggacgagc ccgtgcgcga tcagctcgtg gaccacggcg gggaccccac ctgcccggtg
     6541 gaactcctcg ccgaggtact tgccggccgg ctgcaggtcg acgatcagcg ggacggtgag
     6601 gccgatgcgc tcccagtcgt ccatcacgag cggcacgccg atgtgtcgcg cgatcgcgtt
     6661 gatgtggatg ggagcgttgg tggagccgcc gatcgcggag ttcaccacga tcgcgttctc
     6721 gaacgcctgg cgggtgagga tgtccgacgg cttgaggtcc tcgtgcacca tctcgacgat
     6781 ccgccgaccg gtccggtagg cgatccgggc gcggtcgcgg tgcggggccg ggatcgcggc
     6841 gcagccgggc aggctcatgc cgagcgcctc ggcgagcgcg ttcatcgtgg acgccgtgcc
     6901 catcgtgttg cagtgccccg gcgaaggggc ggaggaggcc accatctcca cgaacgtcgc
     6961 ctggtcgatc tcgcctgcgg ccagcagctc cctggccttc cagacgatcg tgccggagcc
     7021 cgtccgctcg ccgttgtacc agccgttcag catcgggccg ccggacagca cgatcgccgg
     7081 gatgttcacc gtcgccgccg ccatcaggca cgccggcgtg gtcttgtcgc agccggtggt
     7141 cagcaccacg ccgtcaatcg ggtagccgta gagcacctcg accagcccga ggtaggccag
     7201 gttccggtcg agggccgcgg tggggcgctt accggtctcc tggatggggt gcacggggaa
     7261 ctcgaacgcg atgccgccca tctcgcggat gccctcgcgc acccggtcgg cgagctgcag
     7321 gtggtgacgg ttgcacgggg cgaggtcgga accggtctgc gcgattccga tgatcggctt
     7381 accggactgc agctcctccc gcgtgatgcc ccagttcatg taccgctcga tgtacagcgc
     7441 cgtcatcccg gggttgtccg ggttgtcgaa ccacgcctgg ctgcggaggc gtctcggctc
     7501 gtcggcgttc atgcaggggt ctccgatcgt gtcgtcatgg ggtgagctgg ccgccgttga
     7561 tctcgagcac ctggcccgtg atgtagccac tcatggcggg cgacgcgagg aacaacaccg
     7621 cgccgacgca ttcgtcgggc cggccgggcc ggcccatcgg gacactgctg accatggcgt
     7681 ccatctgcgc gtccgtggtg ttccgttcgt ggaacggggt ggtgatgaca ccgggcgcca
     7741 ccgcgttgac ccggatgccc tctggcgcga gctccttggc cagaccgcgg gtgaacgtgc
     7801 tcaccgcgcc cttcgacgtg gcgtacgcga ccgagccgcc ccctccgccc gtgcgcgcag
     7861 cgacggacgt gacgttgatg atcgagccgg acgcctgacg gcgcatcacc ggaacggcct
     7921 gccggctcat cgcgaagacc gacgtcatgt tcacgtccat cacgcggcgg aagtcctcgt
     7981 cggacatctc ggtcaccggg actcggccca ggaggtcgcc cgcgttgttg acgaggacgt
     8041 ccaggcggcc gtgccgggag acgacgtcgt ccacgaggcc ggccgcttcg gccggctgcg
     8101 cgaggtcggc ggctgcgctc gtggccgtcc cgcccgcctc ctggatcgcg gtcacgacct
     8161 tctcggcccc gtcgctgttg cggttgtagt gcaccaccac acgggcgccc tgacgcgcga
     8221 atccctcggc gacggcagcg ccgatcccac tgctcgcccc cgtgacgagg acgacctgct
     8281 cctggaagtc gagcatctcg ttccccatca catgaccgcc ccgagctgcc acggcacgaa
     8341 ttcctcctga ccgaagccca gctcctcgct gcgcgtccgg cgcccggacg cggtgtccag
     8401 gatgagttgg aacagctccc ggcccacctc ctgcaccgac gccgaaccgt ccacgatcac
     8461 cccgcagttg aggtccatgt cctcggacat ccgctcgtac atctccgtgt tcgtggcgag
     8521 cttcaggctg ggggtgggct tgcagcccag caccgagccg cgcccggtgg tgaagcacat
     8581 caccgtggcg ccgcccgcga cgatgccggt gaccgatacc gggtcgtagc ccggcgtgtc
     8641 catgaatgtg aaaccccggt ccgtgacctt ctcggcgtag cggtacacgg cggtcaggtt
     8701 cgtggtcccg cccttcgcca cggcgccgag cgacttctcc aggatcgtgg tgagcccgcc
     8761 cgccttgttg cccggcgagg ggttgttgtc catcgacccg ttgttgtcgg cggtgtactt
     8821 ctcccaccac cggatccgct cgacgagccg gtcggcgacc tcctggctca cggcccgccg
     8881 cgtcagcagg tgctcggcac cgtagatctc cggcgtctcg ccgaggacgg ccgtgccgcc
     8941 gtggcgcacc agcaggtcga cggcagcgcc cagcgccggg ttggcggtga tgccggagta
     9001 cgcgtcggag cccccgcagt tgaggccgag cacgagctcg ctcgcgggca ccgtggtgcg
     9061 gctcacctcg ttggcggccg ggagcatctc gagcagcgcg gcggtgccgg cgcgcacggt
     9121 ggccgcggtg ccaccctcct cctggatcgt catggtccgg acggcctttg tggcgtcgag
     9181 cgtccacttc tcggcgaggc tcgccacctg gttcacctcg cagcccagac cgagcagcag
     9241 gaagccgccg aagttggggt gctcggcgta tccggtgagg gtgcgctgca ggacgtcgaa
     9301 cccctcccct tcgctcgcca tcccgcaccc ggcgccgtgg gtgagggcga cgacaccgtc
     9361 gacgttcggg aagtcgtcga gcaggccggg ccggcggaac tggtcggcga tcagcttcgc
     9421 cgtcgtcgcc gagcagttca ccgaggtgag gatgcccagg tagttgcgcg tggcgaccct
     9481 gccgtcggcc cgcacgatgc cctcgaacgt ggcccgctca cccccgggca ccagctcggt
     9541 caccgtggcg tcgaccccgg cggcgtagtc gcgctcgaac gccttgaact cgacgttgtg
     9601 ggtgtgcacg tggtcgccgg gctcgatggc gacggcggcg aacccgatga tctggttgta
     9661 cttgcggacc ggctcgcccg ccgcgatcgg ggcggtcgcg accttgtgcc cgcggggcac
     9721 ggcatggcgc agggtgagcc cgcccgcggc cgtgccctcc tcgaggtcgc gggaggcgac
     9781 cacgacgttg tcggcggcgt gcaggcgcac gccgatctcg tcgaaggatg gtgccacgct
     9841 catcagctct ctcccgtggt ggtcggggcg taggcccagg gcagcaggtg cccgtgatcg
     9901 tcgaaggtca tcgggacggc gtcgcccgtg atttccagct tggggtcggc cagcacccgc
     9961 tcgcgcaggg actccgacac ccacagctgc tcgaggtcga gggtgttgcg catccggacc
    10021 aacgtgaccc tctcgaggtc ggggcgcccg caggtgagga tcgccgcggc gacggtgctg
    10081 cggtccgtcg gcatcgccat cgggacctgg gcacgctgcg gaccaccgag gcccgaggtc
    10141 atcgcgttga tatagacggc ctgcaggtcg agcttctcca gcaggcggaa cggcacgaag
    10201 tcggcgaggc ccaggccgac ggcgttgccg tgggaggcgt cactgacgtc cagcaccgcg
    10261 atgttggtga tgcgcggccg ctcgaactcg gcactcccgc ggatcatcat ccggccgatg
    10321 acgttggtgt ccatgccgga gccggagaag ttcttgccca tgacgtcgac cacggccacg
    10381 tcgatgtcgt cgaagggcag ggtcgccatc cggcgcttgg cgtcggcgag cagcgcggtc
    10441 tcgaccggcc cgccgatgtc ctcggctccc accagttcga tcctcgccgc ccggtcgccc
    10501 gcgttctcga tgatcgccaa gccaccgagg accttgccgg tctggacgat ccgccgggcg
    10561 acctgcggca cccatacacc cagattcgcg gggccgaacc ggtggatgcc ctcggcgccg
    10621 cgctgcttcc ccagcccgat cgcgacgatc ttggcgagcc cgctctcgac ctcgcccgag
    10681 aagtcggtgt gggccttcac gcggttgacg gcaaggatgg cgtccgcctt ggccgcgtgg
    10741 gcgtcgaggt gcacggcggg gccgccgggc acccgctcca gctcgaccgt ctccatcgtc
    10801 gcgtggatcg gcatgccgag ggactcctcg gtcatcccga ggtttgccag cagctggacc
    10861 tggccgtcgg cggtggcccc gccgtgcgag cccatcgcgg ggacgacgaa cggctcggcg
    10921 ccgaccgagc gcagccactc ccccgcggcc cgcaccacct cgcgcttgtc gtgaataccg
    10981 cggctaccga ccgtgatcgc cacgctcatg ccgggtgcca cccgcgagcg cagcggctcc
    11041 agcgccgcga cggttgccgc ggcgacgtcg tcgatgcgcg tggtgtcgaa gtcctgccgg
    11101 acggggagca gccgggggaa cggcgcatcg aagcgcaggc cgcgcacggc ctcgaacgga
    11161 ccgagatcct tcgtcgtcac agcggttccc cgcacgcccg ggcagcggcg cgggcctgcg
    11221 ccatcgccgc cccgaccgtg acggcctcga cgaggctgtc agcacctgca cgcccggtgc
    11281 cggcgatgtc gaaggcggtg ccgtggtcca cgctcgtgcg cacgatcggc agtccgatcg
    11341 tcgcgttgac cccggtctcg aagccgagga ccttgacggc gatgtggccc tggtcgtgga
    11401 acatcgcgat cgccacgtcg aagtcgcccg cgacggcccg cccgaacagc gtgtcggcag
    11461 gccacggccc gctggcggcg atcccctctg ccttcgcccg atcgatggcg ggggtgatca
    11521 cccgcgcctc ctcgtcgccg aacaggccgc cctcaccggc gtgcgggttg aggcccgcga
    11581 ccccgacgga cggctcgtcg atgcccatgt cgcgcagcgc cctgtgggcc agcctgatcg
    11641 tggtcagtac ccgttcggtg gtgacgcggt cgagcatctg ccggatgccg acgtgggtgg
    11701 tgacgtgcac gactcggagc ttcagcgcgg tgagcatcat cgcgaagtcc gtggtgccgg
    11761 tgaggtcggc gaggatctcg gtgtggccgg ggtaggggtg gccggcgagc gcgagggcct
    11821 ccttgttcag gggagcggtg acgatgccgt cgatctcacc ggcgagcgcg agggccaccg
    11881 cccgctcgac gccctcgaag gcgatctgcc ccgcctcggc ggacaccttc ccgatgggga
    11941 cagcgacgtc ggacggcacg gtgagcacgt cgagcgtccc cggctcgaag cgcgcctcac
    12001 ccggtgcgga cacctcgcgc agcgcgggtg cgtccgggac gaccgcagcg gcggatcgca
    12061 gcgcccggag gctgccgatc gcgaccagcc tcgcgacgtc gacccgcggg tcg
//
LOCUS       DASRSC010000028         9372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_826437,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000028 DASRSC010000000
VERSION     DASRSC010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826437"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1082
                     /gene="sigJ"
                     /locus_tag="VFE39_01235"
     CDS             195..1082
                     /gene="sigJ"
                     /locus_tag="VFE39_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HET6257250.1"
                     /translation="MDADAERFEAHRAHLIRIGYRITGSLADAEDAVQEAWLRLAGLD
                     DKTRGELRDERAWLTTVVGRLCLDRLRSAAARRERYVGPWLPEPLLTTGEADEPLAAV
                     VRDEGVRMAAMVVLERLSPPQRVAFVLHDALSLSFAQIAEVLRCTEATARQHAARARR
                     IVVAADPPPRVHTAEQRAVIGRFAEAMARADGPALLALLHPDAVWVTDSDGRTKAARR
                     TIVGADKVSRLLLGLLERYGPGLTTGWSPVEVNGDLGVRSPAYGQTGPGVNVFTVRDG
                     RIAAMYTVVNPKKLERLPA"
     gene            1228..1590
                     /locus_tag="VFE39_01240"
     CDS             1228..1590
                     /locus_tag="VFE39_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257251.1"
                     /translation="MATLVSCSWCVDFGTMLQHLDGLDVERVQHIDEYPTHRTHPLYE
                     PAERLVLAYADAMTSRALPATVTDAQVAELEAELGRAGLVELTHSIALENMRAPAYHA
                     LGIVDQGFDAACQVRLQG"
     gene            complement(1780..3069)
                     /locus_tag="VFE39_01245"
     CDS             complement(1780..3069)
                     /locus_tag="VFE39_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HET6257252.1"
                     /translation="MAGRHPGGGRTEGGFATGGYTEHDRARLSPEPPKGSEGGERRSS
                     FSRDRARVLHSAALRRLAGKTQVVGPGEGAEISGVPRTRLTHSLEVAQIGRGIAEELG
                     CDPDVVDTAGLAHDIGHPPFGHNGERALDEFGVACGGFEGNAQTLRILTRLEPKISDS
                     GLNLTRASLDASCKYPWVRRPGDRKYGAYGVDAEVFAWVRDGAPGHRTCIEAQVMDWS
                     DDVAYSVHDVEDGIVSGRIDLAALGSPAERSAVVELAATHFGASADALAEAADGLLAL
                     PVVAAVRGFSSATATASAHVALKRLTSELVGRFVLAATDATRAAYGPGPLRRYAADLV
                     VPLRAGAEVALLKAVALRYVMSDPDRLAMQARQRELLAELGSALLAKAPDALDPVFAE
                     DWAGATDDAARLRVVLDQVAVLTDQQAIARHAALSRH"
     gene            complement(3073..3744)
                     /locus_tag="VFE39_01250"
     CDS             complement(3073..3744)
                     /locus_tag="VFE39_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HET6257253.1"
                     /translation="MRGLQAPAPLPVAERGRRGRWVVRLLVGGLLIGVVILGGTAFRV
                     WQVARVDDRRPVDAVVVLGAAQYDGDPSSIFAARLQHAEALYKDGLAPRIITTGGGRP
                     GDVYTEAEAGRRFLIDRGVPAKDVIAVGVGSDTLRSLQAVAARAEQDGWHSAEIVSDP
                     WHSLRARTMARDSGLTAWTSPTRTGPVVQTRETQFRYIVRETGALLYYRLTHASVDTE
                     TSGLG"
     gene            complement(3822..4871)
                     /locus_tag="VFE39_01255"
     CDS             complement(3822..4871)
                     /locus_tag="VFE39_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HET6257254.1"
                     /translation="MSDVTTDSGTGTTPPGSSPLRDLGVALREWATAGVGVVRVLDRH
                     GFGTVESGQLVAGTAGGSVAGALYRGTLDDAVGPLAAAAVGGPRTEEAHVSEAAALDA
                     GLACSGGATLLGHPLPAEPAAALGEALERELPAALVSTTAGAGALVLTGPELGDVHGG
                     LGSPGLDAAAVEAARGLLRRGATVTERVAAEGGELLLDLWVPVPSVLVAGAGAIGGAL
                     IAQAELLGWTARVETELAAVRAAVAAFTDADVLVLLDHAPAFDVVLVEGLRRGRGFIG
                     ALGSRRTQSARRERLLAAGLTEAELAALRGPVGLDLGARTPAETAVSIVAQVVAARSG
                     RAGTALAATDGRIGG"
     gene            complement(4931..5899)
                     /locus_tag="VFE39_01260"
     CDS             complement(4931..5899)
                     /locus_tag="VFE39_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6257255.1"
                     /translation="MRAVRFHAYGGPEVLTVEETPVPRPAEGELLVEVAAVGVTLPVV
                     RLTRGGPDGAGVPLPHVPGGDVVGRVAAVGAGVADWEVGRRVAALAFTGAYAEYVLVP
                     AALAAPVPGGVADEAAVQLVRGGQVALGVLRAAGLQSGESVLVTAAAGGVGHLVVQLA
                     RILGATRVVAAIGRAAGAAKADALRALGADQVITYDELSAGRVQPVDVVLDGVGGDVQ
                     RACLEALAPLGRLVAFSGAGATVDVNELRMHSRTIIGFAMAHFAGGRPEVYTRHRREL
                     WDLHLQGRLRPLVHRVLPLERSPDAHRIVEGRENVGKVLLRPDAGC"
     gene            6079..6702
                     /locus_tag="VFE39_01265"
     CDS             6079..6702
                     /locus_tag="VFE39_01265"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET6257256.1"
                     /translation="MRLTRDRVVAVALDLLDEVGMENLTMRRLADALDVRNGATYWHF
                     RSKQALLEAMADALLAGLPAGLPAGQPWDELVAEMARRLRQALLSRRDGARLFSGSFF
                     PLPNALAYGETMVGLLVGAGLDHCAATWATDTLTYYVVAHVTEEQLAAALPDGGQAAR
                     VRLDAALDPDRHPHLLAARDDLAAPHPIAHFDHGLGLVITGIRSSRG"
     gene            complement(6716..7321)
                     /locus_tag="VFE39_01270"
     CDS             complement(6716..7321)
                     /locus_tag="VFE39_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6257257.1"
                     /translation="MIKVLLADDQALVRGALAAMLGLEPDIEVVAQVGSGAEVVPAAL
                     QAAPDVALLDVQMPGGDGLDAATALRAALPTCRVVVLTTFGRPGYLARAMRAGAAGFV
                     VKDAPPEQLADAVRRVHAGLRVVDPVLAAESLSTGTSPLTDRERDVLLAAADGATVSD
                     VAARLHLSEGTVRNHLSSAIGKTSARTRAEAVRLADERGWL"
     gene            complement(7318..8475)
                     /locus_tag="VFE39_01275"
     CDS             complement(7318..8475)
                     /locus_tag="VFE39_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6257258.1"
                     /translation="MSSITGDADEPVRDPTRLRTKRWYVMAGGWSLLLTPWLYVGATS
                     DHPLVVRVLLVAAVLAYIACYVHGLYVAMYRKQPAFSAALILVMAGLWVAIAAGFGPQ
                     DGAVYTVSYLLVTVIALVPSPWTTVAAVLAFGSAVGIGWLVGDGLTGPDVFGMAAISV
                     AMRGQFWLIRANSQLRDAREELARLAVTAERERMARDLHDVLGHSLTTITVKAGLARR
                     LLEGGDVGRAAEEVTDVEQLGRQALAEVRSTVSANRVASLGRELAGAREALRAAGIEA
                     DLPAAVDDVPADRQQAFAYVVREGVTNTIRHSGASRCTVRLTPTSVEVVDDGVGSPPG
                     TPAGNGLSGLIERLAAVGGWVDAGPTPTGGYRLRAEVVARSAPARRTEPTG"
     gene            complement(8510..9235)
                     /locus_tag="VFE39_01280"
     CDS             complement(8510..9235)
                     /locus_tag="VFE39_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6257259.1"
                     /translation="MGALLLAEVRRLYRSPRFLIFTVGFPVVYFVVFTGIFATGPDAA
                     AASVVLMVNMAAFGAISASISTGGRVAVERSIGWNRQLRLTPLPAWGYVATKAAVAML
                     VALPALLLVFAVAGVAKGVDLDPLTWVRVFLAAWLGVLPFAAIGLLLGLVATPDSAQG
                     MSTVTMLVFSLLGGVLIPAEVLPPGMVSVAHLLPSYWLTAIAQGQAAGDAVPIEGVAV
                     VLAWLLVAGAGVAVRYRRDALRV"
BASE COUNT         1335 a         3615 c         3296 g         1126 t
ORIGIN      
        1 gggtggtctt gcccgcgccg ttgggtccga ccacagcggc cgcccgacct gcgtcgaggg
       61 tgaagccgac accggccagc accactgcgg aatcgaactg cctgaccagg ccgttcacga
      121 ccagcacgcc cgtccacccc cgtttcgacg ctagcaggcc gcaccaggcg cctgacgcca
      181 cgtaggttcg tgccgtggac gcggacgcgg agcgtttcga agcacaccgg gcacacctga
      241 tccgcatcgg ctaccgcatc accggcagcc tcgcagacgc cgaggacgcc gtgcaggagg
      301 cctggttgcg cctcgccggg ctggacgaca agacccgcgg cgagctgcgc gacgagcggg
      361 cctggctgac caccgtcgtg gggcggctgt gtctcgaccg gctgcggtcg gccgccgcac
      421 ggcgcgaacg ctatgtgggt ccgtggttgc cagaacccct gctgaccacc ggcgaggccg
      481 acgagccgct cgcggccgtg gtgcgcgacg agggcgtgcg gatggcggcg atggtcgtcc
      541 tcgagcgact gtcgccgccg caacgggtgg cgttcgtcct gcacgacgcg ctctcgctgt
      601 ccttcgccca gattgccgag gtgctcaggt gcaccgaggc cacagcccgc cagcacgccg
      661 ctcgtgcacg gcgcatcgtc gtggcggccg acccgccacc gcgggtgcac accgccgagc
      721 agcgcgcggt gatcggccgc ttcgccgagg cgatggcccg cgccgacggc cccgcgctgc
      781 tcgccctgct gcaccccgac gccgtgtggg tcaccgactc ggacgggcgg acgaaggcgg
      841 cccgccgcac gatcgtcggc gccgacaagg tgtcgcgcct gctgctcggc ctgctcgagc
      901 gttacggtcc cggcctgacc accgggtggt cgccggtcga ggtcaacggg gacctcggcg
      961 tccggtcccc cgcctacggg cagaccgggc ccggggtcaa cgtcttcacc gtccgcgacg
     1021 gccgcatcgc ggcgatgtac accgtcgtca acccgaagaa actggaacgc ctaccagcct
     1081 gacgagcgtg ccaaattccg cccacgagtc gccccacccc acccgcgagt cgcccctttc
     1141 ccgcccccga gtcgcctcac cccgacccgc gagtcgcctg tccgagtcgg ccaacctgcg
     1201 cgactccggg agctcacggt attccgcgtg gcgacgcttg tgagctgctc gtggtgcgtc
     1261 gacttcggta cgatgctgca gcaccttgac ggcctcgacg tcgagcgagt ccagcacatc
     1321 gacgagtacc cgacgcaccg cacgcacccg ctctatgagc cagccgagcg gctcgtgctc
     1381 gcctacgccg acgcgatgac gtcccgggcc ctcccggcga cggtgaccga cgcccaggtt
     1441 gccgagttgg aggccgagct ggggcgtgcc gggttggtcg agctcaccca ctccatcgcg
     1501 ctcgagaaca tgcgggcacc cgcttaccac gcactcggaa tcgtcgacca gggattcgac
     1561 gcggcatgcc aggtgcgcct gcagggctga ccccggcgga cgaggaccgg cctcattcac
     1621 tggcgtacgc ccgattcagt acgggcgact cccgggccgg aaacggccgg cctgcaacca
     1681 ggagcaggag actcgtggac gcaaaatggg caactcgcgg acccgaaacg ggcgactcgc
     1741 ggacccgaaa cgggcgactc gcggggcggg cctcggtgct cagtggcggg acagcgccgc
     1801 gtgccgggcg atggcctgct ggtcggtcag caccgcgacc tgatccagca ccacccgcag
     1861 ccgcgcggcg tcgtccgtgg cgccggccca gtcctcggcg aataccgggt ccagcgcgtc
     1921 cggggccttc gccagcagcg ccgagccgag ctcggcgagg agctcgcgct ggcgggcctg
     1981 catcgcgagc cggtccgggt cgctcatgac gtaccgcaac gcgacggcct tgagcagggc
     2041 gacctccgcc cctgcgcgca ggggaaccac caggtcggcg gcatagcggc ggagcggccc
     2101 gggcccgtac gccgcgcggg tggcgtcggt ggcggccagc acgaaccggc cgacgagctc
     2161 gctggtgagc cgcttgagcg ccacgtgtgc tgacgccgtg gccgttgccg aggagaaacc
     2221 gcgcaccgcg gcgaccacgg ggagggcgag cagcccgtcg gcggcttccg cgagtgcgtc
     2281 cgcggaagcg ccgaagtgcg tggctgccag ctccaccacg gccgaccgct ccgcggggga
     2341 accgagcgca gccaggtcga tgcgcccgct cacgatgccg tcctcgacgt cgtgcaccga
     2401 gtacgcgacg tcgtccgacc agtccatgac ctgcgcctcg atgcacgtcc gatgtccagg
     2461 cgcgccgtcc cggacccacg cgaacacctc cgcgtcgacg ccgtacgcgc cgtacttgcg
     2521 gtcgccgggc cggcgcaccc aggggtactt gcacgacgcg tccagtgacg cgcgggtcag
     2581 gttcagccca ctgtcgctga tcttcggctc cagccgcgtg aggatccgca gcgtctgcgc
     2641 gttgccctcg aatcccccgc acgccacccc gaactcatcc agcgcacgct ccccgttgtg
     2701 cccgaacggt gggtgcccga tgtcgtgtgc gagcccggcc gtgtcgacga cgtcgggatc
     2761 gcagcccagc tcctccgcga tcccgcggcc gatctgcgcg acctccagcg agtgcgtcag
     2821 gcgggtgcgt ggcacaccgc tgatctccgc accctcgccg ggaccgacta cctgcgtctt
     2881 gcctgccagc ctgcgtagcg cggccgagtg cagcacccgt gcccgatccc gggagaacga
     2941 gctccgccgt tcgccccctt cgctgccctt cggcggctcg ggactgagcc gggcccggtc
     3001 gtgctcggtg tacccgccgg tggcgaaccc gccctcggtc cgtccgcccc ccgggtgtcg
     3061 tccggccacc ggtcagccga gcccgctggt ctcggtgtcg acagaggcgt gggtgagccg
     3121 gtagtacagc agggccccgg tctcgcgcac gatgtagcgg aactgcgtct cgcgggtctg
     3181 caccacaggc ccggtgcggg tgggggacgt ccacgccgtg agcccgctgt cgcgggccat
     3241 cgtccgtgcg cgcagggagt gccacgggtc gctgacgatc tccgcgctgt gccagccgtc
     3301 ctgctccgca cgggccgcga cggcctgcag gctgcgcagg gtgtccgagc ccaccccgac
     3361 cgcgatgacg tccttcgccg gcacgccacg gtcgatcaga aaacgccgcc ccgcctcggc
     3421 ctcggtgtag acgtcgccgg ggcgtccgcc gcccgtggtg atgatccgcg gcgccagccc
     3481 atccttgtag agcgcctcgg cgtgctgcag acgtgcggcg aagatcgagc tgggatcgcc
     3541 gtcgtactgg gcagcgccga gcaccacgac ggcgtcgacc ggtcggcggt cgtcgacccg
     3601 agccacttgc cacacgcgga acgcggtgcc gccgagaatc acgacgccga tcagcagccc
     3661 cccgacgagc agccggacga cccagcgacc gcgcctgccg cgttcggcaa cgggcagcgg
     3721 agcgggcgcc tgcagaccgc gcatcggctc ggtcggcggc tcgtggggcg ggttgggccc
     3781 gtgcggaagg ttcgacggcg ccggaccgat gatccgcccc gtcatccgcc gatccggccg
     3841 tccgtggcgg cgagggccgt gccggcgcgc ccggagcggg ccgccaccac ctgagcaacg
     3901 atcgacacgg cggtctcggc cggggtgcgg gcgcccaggt cgagtcccac cggaccgcgc
     3961 agcgcagcga gctcggcctc ggtgagcccg gccgccagca gccgttctcg acgcgccgac
     4021 tgcgtgcgcc tcgagccgag cgccccgatg aacccgcgcc cgcggcgcag cccctccacg
     4081 agcacgacgt cgaaggccgg ggcgtggtcg agcagcacga gcacgtcggc gtcggtgaag
     4141 gcggcgacgg ccgcgcgcac ggcggcgagc tcggtctcga cgcgggcggt ccagccgagc
     4201 agctcggcct gggcgatcag cgccccaccg atcgcgccgg ccccggccac cagcacggac
     4261 ggcaccggca cccacaggtc cagcagcagc tcgccgccct cggccgccac acgctcggtg
     4321 accgtggcac cgcgccgcag cagcccgcgc gcggcctcca ccgcagcggc gtcgaggccg
     4381 ggcgagccga gcccgccgtg cacatcgccg agctcggggc cggtgagcac cagcgctccg
     4441 gcgcccgcgg tggtggaaac cagcgcggcg ggcagctcgc gctccagcgc ctccccgagc
     4501 gcggcggcgg gttccgcggg cagcgggtgg ccgagcagtg tggcgccacc gctgcacgcg
     4561 aggccggcgt cgagcgctgc ggcctccgag acgtgggcct cctcggtgcg cggcccaccc
     4621 accgcggcgg cagccagcgg accgacggca tcgtccaggg tgccgcggta cagggcgccg
     4681 gccacggagc ccccggcggt gcccgccacc agctgcccgc tctcgaccgt gccgaaaccg
     4741 tggcgatcca gcacccggac gacccccaca cccgcggtcg cccattcgcg cagcgccaca
     4801 ccaaggtccc gtagtggcga agatcccggt ggcgtcgttc ccgtcccgga atcggtagtc
     4861 acgtcgctca cgatcgccag ggtagccatc gatcaggttg tagcggcggc acgtggcggc
     4921 gcgtcagtgg tcagcagccg gcgtccggcc ggagcagcac cttaccgacg ttctcccggc
     4981 cctcgacgat gcggtgggcg tcgggcgacc gctccagggg aagcacccgg tggacgagcg
     5041 ggcggaggcg gccctggagg tgcaggtccc acagctcccg gcggtgccgg gtgtagacct
     5101 cgggccgacc gccggcgaag tgggccatgg cgaacccgat gatcgtcctg gagtgcatgc
     5161 gcagctcgtt gacgtcgacg gtggcgcctg cgccgctgaa ggcgacgagc ctgccgagtg
     5221 gggcgagggc ctcgaggcag gccctctgga catcgccgcc gacgccgtcg aggacgacgt
     5281 cgaccggctg cacacggccg gcggacagct cgtcgtaggt gatcacctgg tcggcgccga
     5341 gcgcccgtag cgcgtcggcc ttggcggcac cggcggcacg cccgatcgcc gcgaccacgc
     5401 gggtggcccc gaggatccgg gccagctgca ccaccagatg cccgacgccg ccggcggccg
     5461 cggtgaccag gacgctctcc ccgctctgca gcccggccgc acgcagcacg ccgagcgcca
     5521 cctgtccacc gcgtaccagc tgcacggccg cctcgtcggc gaccccgccg ggaaccgggg
     5581 ccgcgagcgc agcgggcacc agtacgtact cggcgtaggc gccggtgaag gcgagggccg
     5641 ccacccgccg gcccacctcc cagtcggcca cgccggcgcc gacggccgcc accctcccga
     5701 cgacgtcacc accgggcaca tgcggcagcg ggacgccggc gccgtccggg ccaccccggg
     5761 tgagccgtac gaccgggagg gtgacgccga ccgccgcgac ctcgaccagg agctctccct
     5821 ccgcgggccg gggaaccggt gtctcctcga cggtcagcac ctccggcccg ccgtaggcgt
     5881 ggaaccgcac cgcgcgcatg tggcctcctc gtgatcgtcg gaacgggacc tggccgcgcc
     5941 cggggagggc gtggcccgca gacgtcacgt cgtgttgcgg atgagcactc gtcctccttc
     6001 ttcaacactg ttgaacttca acgccggtga agaatgcggc acactccacg gtccggcaag
     6061 cagatttcgg gaggccacgt gcggctcacc cgcgaccgtg tggtcgccgt cgccctcgac
     6121 ctcctggacg aggtcggcat ggagaacctg accatgcgca ggctcgccga cgcactggac
     6181 gtccggaacg gggccacgta ctggcacttc cgcagcaagc aggccctgct ggaggcgatg
     6241 gccgacgcgc tgctcgccgg gctccccgcc ggcctccccg cgggccagcc gtgggacgag
     6301 ctcgtcgccg agatggcacg tcggctacgc caggccctgc tgtcccgccg cgacggcgcc
     6361 cgcctgttct ccggaagctt ctttcccctg cccaacgccc tcgcctacgg cgagacgatg
     6421 gtggggctgc tggtgggagc cgggctcgac cactgcgcgg cgacctgggc caccgacacc
     6481 ctcacgtact acgtcgtcgc ccacgtcacc gaggagcagc tcgccgccgc tctccccgac
     6541 ggcgggcagg ccgcccgcgt ccggctcgac gcggcgctcg atcccgaccg ccatccgcac
     6601 ctgctcgctg cgcgggacga cctcgccgcc ccacacccca tcgcgcactt cgaccacggg
     6661 ctggggctcg tcatcaccgg gatccgcagc agccggggct gatccaggag tcgggctaca
     6721 accagccgcg ctcgtccgcc agccggacgg cctcggcgcg ggtgcgggcc gaggtcttgc
     6781 cgatcgccga ggagaggtgg ttgcgcaccg tgccctccga caggtgcagc cgcgccgcca
     6841 cgtccgagac ggtggcgccg tcggccgcgg cgagcaggac gtcgcgctcc cggtcggtca
     6901 gcgggctcgt gccggtggag agcgactccg ccgcgagcac cgggtccacc acccggagcc
     6961 ctgcgtgtac ccggcgcacg gcgtcggcga gctgctcggg cggggcgtcc ttcaccacga
     7021 accccgccgc cccggcccgc atggcccggg cgaggtagcc gggcctgccg aacgtggtga
     7081 gcacgacgac gcggcaggtc gggagggccg cgcgcagcgc cgtggcggcg tcgagaccgt
     7141 cgccgccggg catctggacg tcgaggagcg cgacgtccgg ggcggcctgc agagcggcgg
     7201 gcaccacctc ggcgcccgaa ccgacctgcg ccaccacctc gatgtcgggc tccaggccga
     7261 gcatcgcggc cagcgccccc cggacgagcg cctggtcgtc ggcgagcagc accttgatca
     7321 tccagtcggc tccgtccgcc gggccggggc gctgcgcgcc acgacctcgg cccgcaggcg
     7381 gtagcccccg gtgggcgtcg gtccggcgtc gacccagccg ccgacggcgg cgagccgctc
     7441 gatgagcccg ctgagcccgt tgcccgcggg cgtaccgggc ggactgccaa caccgtcgtc
     7501 gacgacctcc accgaggtcg gtgtgaggcg caccgtgcac cggctcgccc cactgtgccg
     7561 gatggtgttg gtgacgccct cccgcaccac gtacgcgaag gcctgctgac gatcagctgg
     7621 cacatcgtcc acggcggcgg gcaggtcggc ctcgatgccc gcggctcgca gggcctcccg
     7681 ggcaccggcc agctcgcgcc cgagcgaggc cacgcggttc gccgacaccg tggagcgcac
     7741 ctcggccagc gcctgcctgc cgagctgctc gacgtcggtc acctcctcgg ccgcccgccc
     7801 gacgtcgccc ccctccagca gccgccgggc cagcccggcc ttgacggtga tcgtggtgag
     7861 gctgtgtccg agcacgtcgt gcaggtcgcg ggccatccgc tcacgctcgg cggtgacggc
     7921 cagccgggcc agctcctcac gggcgtcgcg cagctgggag ttcgcccgga tgagccagaa
     7981 ctgtccccgc atcgcgacgc tgatcgcggc catgccgaag acgtccgggc cggtcaaccc
     8041 gtcgccgacc agccagccga tcccgaccgc gctcccgaac gcgagcacgg ccgccacagt
     8101 ggtccacggt gacgggacca gcgcgatgac cgtcacgagg aggtagctga cggtgtagac
     8161 cgcgccgtcc tgcggcccga acccggcagc gatcgccacc cacagtccgg ccatcacgag
     8221 gatgagcgct gcggagaagg ccggctgctt gcggtacatc gccacgtaca gcccatgcac
     8281 gtagcaggcg atgtaggcaa ggaccgccgc caccagcagc acgcggacga cgagcgggtg
     8341 gtcggacgtg gcgccgacgt acagccacgg cgtgagcagg agcgaccacc cgcctgccat
     8401 cacgtaccac cgcttggtgc gcagccgtgt ggggtcgcgg actggctcgt ccgcgtcccc
     8461 ggtgatcgag gacatgccga tcaccgtaac ccggtcgccg cctccggatt cagacacgga
     8521 gcgcgtcacg ccggtaccgg accgcgacac cggcccccgc gaccaggagc cacgccagca
     8581 cgacggccac gccctcgatc ggcacggcgt cgccggccgc ctgcccctgc gcgatggccg
     8641 tgagccagta gctgggcagc agatgggcca cggagaccat ccccggcggc agcacctccg
     8701 ccgggatcag caccccgccc agcaggctga acaccagcat cgtcaccgtc gacatgccct
     8761 gcgccgagtc cggcgtggcg accaggccga gcagcagccc gatcgcggcg aacggcagca
     8821 ccccgagcca cgcggcgagg aacacccgaa cccaggtgag cgggtcgagg tccaccccct
     8881 tcgccacgcc cgccaccgcg aacaccagca ggagcgcggg cagcgccacc agcatcgcga
     8941 cggccgcctt ggtggcgacg tacccccatg cggggagcgg ggtcagccgc agctggcggt
     9001 tccagccgat gctgcgctcc accgccaccc gtccgccggt gctgatcgac gccgagatcg
     9061 caccgaacgc agccatgttc accatcagca ccaccgaggc ggccgccgcg tccggtccgg
     9121 tcgcgaagat cccggtgaag acgacgaagt agacgacggg aaagcccacc gtgaagatca
     9181 ggaaccgcgg tgaccgatag agccggcgca cctcggcgag cagcagcgca cccatggtca
     9241 gttcccctcc gatgcggtca gcgcgacgaa cgcgtcctcg agaccgtgtg ccgagacctc
     9301 gatgtcgcgg gcctgcggat atccgacgac cagtgcgcgc agcgcagcgt cggagtcgga
     9361 acagtcgagc cg
//
LOCUS       DASRSC010000029        13772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4266684,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000029 DASRSC010000000
VERSION     DASRSC010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 13772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13772
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4266684"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFE39_01285"
     CDS             complement(<1..193)
                     /locus_tag="VFE39_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsI"
                     /protein_id="HET6257260.1"
                     /translation="MVALAVATVKLVAIQTVQAGALSAASARQSTTDILLPAERGEIL
                     DRNGSPLAFSVDARALVTNP"
     gene            complement(331..1335)
                     /locus_tag="VFE39_01290"
     CDS             complement(331..1335)
                     /locus_tag="VFE39_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257261.1"
                     /translation="MSAPVTERTRSASRGATRTRSTTIPAQRGAPAAMPRAWEGEPAP
                     AKGTQRSARKAYARRDDRLRRVVGGRPARTGAPTRRAQFVLLIMVLLAVGLVATLWLS
                     TAAAAGSYHLQDARTAALQLSQQSQRLHREVADLESAPELARRAQALGMVPVQDAARL
                     VVNPDGSVTVVGEPRAAVPPPPPAPPASAPGAPPGAPVPPAAGVAPLDGTTPPVDPQQ
                     LADIAPVAPGDLVAQHTGTEPRTGTGQGAAAGQGAAAGQGAAAGQGAAAGQGAAAGQG
                     AAAGQGNGTAQGAGAGAGAAQQGAGTAQGAGTAQQGTGTAAGQGAGTGQSTAAGAGTD
                     "
     gene            complement(1554..2537)
                     /gene="rsmH"
                     /locus_tag="VFE39_01295"
     CDS             complement(1554..2537)
                     /gene="rsmH"
                     /locus_tag="VFE39_01295"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET6257262.1"
                     /translation="MGQHDHENEPAGARHVPVALPRVAELFRPALADRPAVVVDATLG
                     LGGHAAALLATHPQLTLVGLDRDPEALALAGRRLVAHADRVHLVHAVYDRIADVLGEL
                     GLPRVDGVLFDLGVSSLQLDAEERGFSYSRDADLDMRMDPTTGLTAAEILNTYSVPQL
                     ARVLREYGEERFAGRIAAAVARRRAQGALRRSAELVELLYEAVPAASRRTGGHPAKRT
                     FQALRIEVNGELDALQTALPAALDSLAVGGRIVVLAYHSLEDRIVKRELTARSSSRTP
                     VDLPVELPGHGPEFRLLTRGSEQASEDEINDNPRAASVRLRAAERIREVAA"
     gene            complement(2701..3132)
                     /gene="mraZ"
                     /locus_tag="VFE39_01300"
     CDS             complement(2701..3132)
                     /gene="mraZ"
                     /locus_tag="VFE39_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HET6257263.1"
                     /translation="MFLGTYTPKLDDKGRLTLPAKFRDELRGGLMITKGQDHCLYVFT
                     REAFTELASKVASAPLTSESARVFQRNLFSGTDEQNPDGQGRIAITPELRRYAGLSKD
                     CVVIGAFTRAEIWDAEAWQRYQDEHEDTYAQAQEEVLPGLL"
     gene            3575..4543
                     /locus_tag="VFE39_01305"
     CDS             3575..4543
                     /locus_tag="VFE39_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6257264.1"
                     /translation="MEGDAAGLADIADLGGRIAANIQRVIVGKPEVVRVALVTLLAEG
                     HLLVEDVPGVGKTSLAKALARSIDCSVSRVQFTPDLLPGDITGMSIYNRQTTEFEFRP
                     GPVFANIVIADEINRASPKTQSALLECMAEHQVTVDGATYGLASPFMVVATQNPIEMD
                     GTYPLPEAQRDRFTTRVSVGYPDLAAELAMVDEHAASDPLETLRPVVDARLVRSVIEG
                     VRRVHVSPEVRQYCVELVSTTRRLPELRLGASPRATLQLVRAARAQAALAGRGFVVPD
                     DVQAVAVPVLAHRLLLAPDALASRRSAVDIVRSLLGRHAVPARPGV"
     gene            4587..5855
                     /locus_tag="VFE39_01310"
     CDS             4587..5855
                     /locus_tag="VFE39_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6257265.1"
                     /translation="MSEPAGRFSGLTTRGRCLLAGGVATGASAIALNERDLLRVGVFV
                     ALLPLLALLLAARARRTIHVERELTPARVPVGAAVTVGLRLQAGSLLGALRLADTAPD
                     AAGPQATAPPRFTVHRLSTRGGALLTYPLRPVLRGVHRVGPLRGTAGDPLGLAEFERE
                     LAPADRLLVLPRVVPLRGLPPAIGAGEGTPGAALAHQGQGASDVLIRPYRQGDELRRV
                     HWRSTARHDELMVRLEERPWRGGMTVLLDRRDSAHRGRGADSSLEFAVSLTASICTHL
                     LARGEPLVLVTEDGVQLSGPGSGTGPEPLLDALAALRPSARPDLSGPELRSGTDVLAV
                     LGALGPGQVEALLGRRTGGGNAVLLDTTTWDPAVRDHAGGGSVTAANAATLRKAGWHV
                     TVATAGSSPDRVWDNMVAGSAPDGLRPGVS"
     gene            5944..8220
                     /locus_tag="VFE39_01315"
     CDS             5944..8220
                     /locus_tag="VFE39_01315"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HET6257266.1"
                     /translation="MTTSVRVPPAAPPSAAPPPVTRGGRLPWVATLASGLAVLLAAGP
                     MSAIVQGSGWFGHAATVVTVVVAAGLLLHRLGPIVVAAGQAAAVLVLLTVQFADHGLL
                     GVLPGPAAFGEFGGLLSGAGQQIDTGTAPVPATPEILFLVTAAFGLLAIGVHLAAVSA
                     AAPAAAGVPLLAVFAVPAALTDVLLPWWAMAAAAAGFGLLLVAETGIRRRIPGGIALV
                     AVAVVLAVGVGAAASFVGTAGRFDNGSGSGGSGGSIGLSPFTALRGQLEQTTPTELFR
                     VKGLTRPTYLRALTLRQYVPDAGWQATRPEPGVGLPGPIQAQPGIAGDLADVEIENVA
                     FRDYWLPLYGEPLDVGGLPEGPWLYDKSSDTGYTGRPRQENGWREQAFLPAPTVTQLR
                     QSEGSAGVGPEYLDVTGVDPRVGEIAKQVVAGRNNNFDRAMALQDYFTGPNSQFTYSL
                     QTAPGAGDDALVEFLTVGRAGYCEQFASAMAVMLRTVGVPARVAVGFTAGIDDGQYRS
                     ISTSDAHAWVEAWFPGVGWTIFDPTPLTDGRTIAPPYVQEAQGDNPGAGKAPAEQDLL
                     PGQEAGQEPLPAPAEPEQLPPPPVAPSATEGGGLPLWPIAVVLLVAVAALVPAGLRAL
                     ARRRRMAAVGAGGPGAAGAGWAEVLAESADRGVTYPPSDTVRGAARRLVREHKLDGDA
                     QQALRQVVGAVESSWYGDVHPAPGELQEPVRAVRAGIASESALSLRGRLLPRSVVHRT
                     PSEQLARAAAREPADHRS"
     gene            complement(8272..8655)
                     /locus_tag="VFE39_01320"
     CDS             complement(8272..8655)
                     /locus_tag="VFE39_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014467437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HET6257267.1"
                     /translation="MPLSEHEQRLLEQIERALYAEDPKFASTVRGGRLRKPTRRRRLQ
                     GVGLFVLGLVLLVVGVAVPQLWLASSFPVLSVVGFLVMLGGAVLAVTSVGSGGQPGQP
                     KDAEPDKNRFTGRMEERFRRRFEQE"
     gene            complement(9058..9825)
                     /locus_tag="VFE39_01325"
     CDS             complement(9058..9825)
                     /locus_tag="VFE39_01325"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6257268.1"
                     /translation="MVSGATADTAGAVGGGSVHRVLVAEVARVRAVRPDRPPRVLDVG
                     GGSGAWAVPLARSGCAVTVVDNSPNALAALHRRAREAGVHDLVTPLQGDVDALADVVP
                     AGGADLVLGHGLLEVVDDAAAAVSALTTATVAGGAVSVLVVGRFAAVLARTLSGKLAE
                     ARAVLTDPHGRFGTDDALRRRLDAATLRSLLEDGGALRVEVLQGDGVLEGWVPSAVRD
                     GGPAAARAVAELEDLAASAAPLVEMAARLHVLARRAG"
     gene            9880..10785
                     /locus_tag="VFE39_01330"
     CDS             9880..10785
                     /locus_tag="VFE39_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HET6257269.1"
                     /translation="MQVVATLSLKGGVGKTTVALGLAGAALHHGVRALVVDLDPQANS
                     TIALDVGTTTATVADVLDDPRPSVVRRAIAPSGWGDGLDVLVGAEQTERHNHPDPGPE
                     QLIRLARALEQLDDVEQVEGRARRGESNGQAAANGNPGDGHAHDEPYRLVIIDCPPSL
                     GQLTRSALAAAHRAVLVTDPTIFAVSGVQRAFDAIQTERQRGNPDLQPLGVLINRVRP
                     RNTEHEFRIHELRELFGPLVFTGVLPDRSAIQQAQGACMPIQRWDTPGAREASAVFTL
                     LLGRVLRSERTQRVVGATTAPPATR"
     gene            complement(10828..11256)
                     /locus_tag="VFE39_01335"
     CDS             complement(10828..11256)
                     /locus_tag="VFE39_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAV_6107 family HEPN domain-containing protein"
                     /protein_id="HET6257270.1"
                     /translation="MTITTDRVPYPRAPVRPAAPTSRAALGLLRQAAAGLVEAHRTEE
                     PLLRYPAAYLAALRAAAAVLAVHARPQPRRGSTRNVWHLLAEVAPELQEWAAFFASCS
                     DTRAAAEAGIARLVGRRDADDLLRQAEQFVGIVGASVPLR"
     gene            complement(11240..11593)
                     /locus_tag="VFE39_01340"
     CDS             complement(11240..11593)
                     /locus_tag="VFE39_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257271.1"
                     /translation="MSAFGRVFGSRQPTPIQVRWQDGEPIEFQRRPVRRGPRYLVTDV
                     RERWIEESPWPFPSPMPVGGDRLRCWRVVARSAGEPEAPEREFVLRMRAGPGVWDLVR
                     VAERESPGGHDDHYH"
     gene            11780..12355
                     /locus_tag="VFE39_01345"
     CDS             11780..12355
                     /locus_tag="VFE39_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6257272.1"
                     /translation="MTRAPARQLVQLGPGDLADRLTEALHVYVSAMGYPTSTARHRRT
                     LWLDHTRRPGWRAAGWIDAAGTLLGIAYGYTGGPGQWWFEEVRRGLSAAGPEAQGWIR
                     DYFELTELHVRPDAQGHGLGEGLLRALLDGTDRPRVLLSTPEYGAQQPGRAWRLYRRT
                     GFRDVLRNHRFTGDSRPFAVLGRELPLGSAP"
     gene            12403..13086
                     /locus_tag="VFE39_01350"
     CDS             12403..13086
                     /locus_tag="VFE39_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3153 domain-containing protein"
                     /protein_id="HET6257273.1"
                     /translation="MSGVPTPTASRRRVLPLLLLILLVAGTALGGCARVRTALAVQPD
                     DSVTGEMILATPEKGPDDTGPAVTVPPELKSDVAVSPYRQDGYTGSVLRFSGLTFAEV
                     ATLSRVAPPAADAVQFELRRAGNRVLVSGAVDLTTVSVDKADFQLKIGFPGQVLDSNG
                     DADSSTVSWTFTPGQVGDINAVVAYDDPNAPSAVNWTLLLGIVVALAAGAVLVLAHRT
                     RNPPVSPGR"
     gene            complement(13070..>13772)
                     /locus_tag="VFE39_01355"
     CDS             complement(13070..>13772)
                     /locus_tag="VFE39_01355"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET6257274.1"
                     /translation="DTTLVPMAHLTAVSHSVAELRQVIGAYAAAGISNILAVRGDPPG
                     DPLGEWVPHPQGLAYADELVSLVRRLGDFCVGVAAFPYGHPRSPDAEFDLARLLAKIR
                     AGADFAISQLFLEPEGFLRLRDRLAAAGIDVPLLPGIMPLTTVRTLHRGPELSGAPLP
                     PALVERLQPYSDDPAAFRAAGMDVTAEMCARLLDEGVRGLHFYTLNRSTATVDLVRRL
                     GLAARRPAPAVSAPG"
BASE COUNT         1836 a         5110 c         5010 g         1816 t
ORIGIN      
        1 gcgggttggt caccagcgcc cgtgcgtcga cactgaacgc cagcgggctg ccgttccggt
       61 ccaggatctc gccacgctcg gccggcaaca ggatgtccgt cgtcgactgg cgcgccgagg
      121 ccgcgctcag ggcccctgcc tggacggtct ggatggcgac gagcttgacg gtggccacgg
      181 cgagcgcgac cacgagcagg atccggccga tcttcaggcg ccgggagtag ggggtacccc
      241 cggtcgcgcg accacggggt gccgggggcc gcggcggcgg ggtcctccgg accgcccctg
      301 cggcccgggg ccggggcggg gccgtagtca tcagtcggtg cccgccccgg ccgcggtgct
      361 ctgccccgtg cctgcgccct gcccggccgc ggtgccggtg ccctgctgcg ccgtgcctgc
      421 accctgagcg gtgcctgcac cctgctgagc agcgccggcg ccggcgccgg cgccttgcgc
      481 ggtgccattg ccctgtccag ctgcggcgcc ctgtccagct gcggcgccct gtccagctgc
      541 ggcgccctgt ccagctgcgg cgccctgtcc agcagcggcg ccctgtccag cagcggcgcc
      601 ctgtccggtg ccggtacggg gctcggtgcc ggtgtgctgc gcgaccaggt cgcccggtgc
      661 gaccggtgcg atatctgcga gctgctgcgg gtcgacgggc ggtgtcgtgc cgtccagagg
      721 tgccacgccg gcggccggcg gcaccggggc accgggagga gcacccggag cggaggcagg
      781 cggggcgggc gggggcggtg ggaccgccgc ccgcggctcg ccgacgacgg tgacggaacc
      841 gtccggattg accaccaggc gcgcggcgtc ctgcaccggc accatgccga gtgcttgggc
      901 gcgccgagcc agctccggtg ccgactccag atcggccacc tcgcggtgca ggcgctggct
      961 ctgctggctc agctgcagcg ccgccgtcct ggcgtcctgc aggtggtacg agccggcggc
     1021 ggccgcggtg gacagccaca gcgtcgccac cagcccgacg gcaagcagca ccatgatcaa
     1081 caggacgaac tgcgcccgcc gcgtgggagc gccggtacgc gccggacgac cgccgacgac
     1141 acgacggagc cggtcgtcgc gccgggcgta ggccttccgc gccgaccgct gggtgccctt
     1201 cgccggggcc ggctcaccct cccaggcgcg tggcatggcg gcgggtgcgc cgcgctgggc
     1261 cgggatggtc gtggagcggg tgcgcgtggc accccgggac gcactgcgcg tgcgctcggt
     1321 gaccggcgcg ctcacaacgc acccccgctg agcccgatcg gcgcttcacc caggccggga
     1381 gatccgctcg cgagcaccgt cgtcgatccc ccgtccacgc gcgccgatcc cgttcgccat
     1441 cccgttcggc gcacatcgac ctgcatgtcg cccccttcct cgtcgacgct cgtgagcgcc
     1501 gttcgccgtg accggtaggg cgacgacgtg tcgcgacggt tcctccacca ccgtcacgcc
     1561 gccacctccc ggatgcgttc cgcagcccgc aggcgcaccg acgcggcccg cgggttgtcg
     1621 ttgatctcgt cttcgctcgc ctgctcggac ccacgggtca gcagccgaaa ctccgggcca
     1681 tgccccggca gctcgacggg aaggtccacc ggcgtccggg agctcgaccg tgccgtcagc
     1741 tcgcgcttga cgatgcggtc ctccagggag tggtaggcga gcaccacgat ccgcccgccg
     1801 acggcgagcg agtccagagc ggcgggcagc gcggtctgca gtgcgtccag ctcgccgttg
     1861 acctcgatcc gcagcgcctg gaacgtccgc ttcgcggggt gtccgcccgt gcgccgggac
     1921 gccgcgggca cggcctcgta gagcagctcg accagctcgg cgctgcgccg cagggcgccc
     1981 tgcgcccgcc gacgggcgac ggccgcggcg atccgccctg cgaagcgctc ctcgccgtac
     2041 tcccgcagca cccgcgcgag ctgcggcacg gagtaggtgt tgaggatctc ggccgcagtg
     2101 aggccggtgg tggggtccat ccgcatgtcg aggtcagcgt cgcgggaata ggagaagccg
     2161 cgctcctcgg cgtcgagctg gagcgaggac acaccgaggt cgaacagcac gccgtcgacc
     2221 cggggaagcc cgagctcccc gagaacgtcg gcgatgcggt cgtagacggc gtgcaccagg
     2281 tggacgcggt cggcatgggc caccaacctg cggccggcaa gcgccagcgc ttccgggtca
     2341 cggtcgagcc cgaccagggt gagctgtggg tgcgtcgcga gcagggctgc ggcgtgcccg
     2401 ccgagtccga gcgtggcgtc caccacgacc gcggggcgat cagccagcgc cggccggaac
     2461 agctcggcga cccggggcag ggccacggga acgtgcctgg ccccagccgg ctcgttctcg
     2521 tggtcgtgct gccccaccgc acacctccct gccccgtgtc gtcgagcacc gcaggccagc
     2581 gctccccagc gggcccggcg gcgatgtccc cacccggcag acctggcacc ggggaaggtg
     2641 cgccagggct gccgggtggg aacaccgccg agttgatctc cacgccgcgt ccgtcccgga
     2701 tcagagaagt ccgggaagca cctcctcctg tgcctgggcg taggtgtcct cgtgctcgtc
     2761 ctggtaccgc tgccatgcct cggcatccca gatctcggcg cgcgtaaacg caccgatcac
     2821 cacgcagtcc ttgctgagcc cggcatagcg gcgcagctcg ggggtgatgg cgatgcgccc
     2881 ctggccgtcg gggttctgct cgtcggtgcc ggagaacagg ttgcgctgga agacgcgggc
     2941 cgactcactc gtgagcggcg cggaagccac cttgctggcc agctcggtga aggcctcgcg
     3001 ggtgaacacg tacaggcagt ggtcctgccc cttcgtgatc atcagcccgc ctcgcagctc
     3061 gtcgcggaat ttcgcgggca acgtgagccg ccccttgtcg tccagtttgg gggtgtaggt
     3121 gccgaggaac atcggcccca cctccacctg tggccccgtc gtgccccagc gcgcaccaca
     3181 ttaccccact ctccaccacc gtcaaccgga acgatgggag tgcattcgcg gaaagggaac
     3241 ggaaagcgca ggtcaagtgg ggtgggggaa ggtggggtgt cctgagtggt caccgccggt
     3301 gcgtgctcga tgcgccggca atttccgtca ctgaccgtga tcaaaaggtg cggagatact
     3361 cctggcggcc ccctgatggc cccctggaac cgccgatcgg ggtgccatgg ggaacgcatc
     3421 cgtcggcagt ggggcacagt ggggaactgg tcggttaccc catcgggcaa agttctcggc
     3481 ggtcgtttga cagactgatg ccgcgtctcg acagatcggg accacacgac gagcgcgagg
     3541 aggtcgggtg accgcgacga gcagccgcgc cgggatggag ggtgacgccg ccggcctcgc
     3601 cgatatcgcc gatctcggcg gtcggatcgc agccaacatc caacgtgtaa tcgtgggcaa
     3661 acccgaggtc gtgcgggtcg cgctggtcac cctgctcgcc gaggggcacc ttctggtcga
     3721 ggacgtgccg ggcgtcggta agacctcgct ggccaaggcg ctcgccaggt ccatcgactg
     3781 ctcggtcagc cgcgtccagt tcacccccga cctgctgccc ggcgacatca ccggcatgtc
     3841 gatctacaac cggcagacca ccgagttcga gttccggccc ggcccggtgt tcgccaacat
     3901 cgtgatcgcc gacgagatca accgcgcctc cccgaagacg cagtcggcgc tgctcgagtg
     3961 catggcggag caccaggtca cggtcgacgg cgccacctac ggcctggcga gcccgttcat
     4021 ggtcgtcgcg acgcagaacc cgatcgagat ggacggcacc tacccgctgc cggaggcgca
     4081 gcgcgaccgc ttcaccaccc gcgtgagcgt cgggtacccc gacctcgctg ccgagctggc
     4141 gatggtcgac gagcacgccg cctccgaccc cctcgagacc ctccgcccgg tggtcgacgc
     4201 acggctggtg cggtcggtca tcgaaggcgt gcgccgggtc cacgtgagcc ccgaggtgcg
     4261 gcagtactgc gtcgaactcg tcagcaccac ccgcaggctg cccgagcttc gcctgggcgc
     4321 ctccccccgg gccacgctgc agctggtgcg tgcagccagg gcgcaggcgg cgctggccgg
     4381 gcgcggcttc gtggtgcccg acgacgtgca ggcggtggcc gtacccgtgc tggcccaccg
     4441 gctgttgctc gccccggacg ccctggcctc ccgccgttcg gccgtcgaca tcgtccgcag
     4501 cctcctcggg cggcacgccg tgccggctcg cccgggcgtc tgacgggcgt gggccggacc
     4561 atggcgggcg ggggcggagc cgcctcgtga gcgagccggc gggccggttc tccggcctca
     4621 ccacccgagg gcgctgcctg ctcgcgggcg gggtggccac cggagcctcc gccatcgccc
     4681 tcaacgagcg cgacctgctg cgcgtcgggg tgttcgtcgc gctgctaccg ctactggcac
     4741 tgctcctcgc ggcccgcgcc cgccggacga tccacgtcga gcgcgagctc accccggcca
     4801 gggtgccggt cggcgcagcg gtgaccgtcg ggctccggtt gcaggccggc tcgctgctcg
     4861 gagcgctccg gctggccgac accgcacccg atgccgcagg cccgcaggcc accgctcccc
     4921 cgcggttcac ggtgcaccgc ctgtccaccc ggggcggcgc cctgctcacc taccccctcc
     4981 gcccggtgct ccgcggagtg caccgcgtcg gcccgctccg cggcacggcg ggcgacccgc
     5041 tcgggctcgc ggagttcgag cgcgagctcg cccctgccga tcggctgctc gtcctgcccc
     5101 gcgtggtccc gctgcggggc ctgccccccg ccatcggcgc aggcgagggc acgcccggag
     5161 cggcgctcgc ccaccagggc cagggcgcgt cggacgtgct gatccgcccc taccggcaag
     5221 gcgacgagct gcgcagggtg cactggcgca gcacggcccg ccacgacgag ctgatggtgc
     5281 ggctggagga acggccgtgg cgcggtggga tgacggttct gctcgaccgt cgtgactcag
     5341 cccaccgcgg gcgcggtgcc gactccagcc tcgagttcgc ggtgagcctc acggccagca
     5401 tctgcaccca tcttctggca cggggtgaac cactcgtcct cgtcaccgag gacggcgtgc
     5461 agctcagcgg acccggctcg ggcacgggtc cggagccgct gctcgacgct ctcgccgcgc
     5521 tgcgcccctc tgcgcgcccc gacctcagcg gcccggagct ccgatcgggc accgacgtgt
     5581 tggccgtgct cggggcgctc gggcccggtc aggtcgaggc actgctgggc cgccgcaccg
     5641 gcggcggcaa cgcagtgctg ctggacacca ccacgtggga cccggccgtc cgagaccacg
     5701 cggggggtgg cagcgtgacg gccgcaaacg cggcgacgct gcgcaaggcc ggatggcacg
     5761 tcaccgtcgc gaccgccggc tcgtcaccag atcgggtctg ggacaacatg gtggccgggt
     5821 cggcacccga cggcctgcgg ccgggcgtct cgtgagcgcg ccggctcccg gccgcgcggg
     5881 caccacgcgc gcgaccaccc ggagcgccgc cgaccgaacc gcgaccgcga cccaggagac
     5941 tccgtgacca cgtccgtccg ggttccgccg gccgcaccgc cctccgctgc gccgccccca
     6001 gtgacacgag gcgggcggct gccgtgggtg gccacgctcg cgtccgggct ggccgtgctg
     6061 ctcgcggccg gccccatgtc agccatcgtg cagggcagcg gctggttcgg ccatgccgcg
     6121 actgtggtca ccgtcgtcgt cgccgccggc ttgctgctgc accgcctcgg ccccatcgtg
     6181 gtcgccgccg ggcaggctgc cgcggtcctc gttctgctca ctgtgcagtt cgccgaccac
     6241 gggctactcg gcgtcctgcc cggcccagcg gcgttcggcg agttcggggg gctcctgtcc
     6301 ggggcgggcc aacagatcga cacgggtacc gcgcccgtgc cggcgacgcc cgagatcctg
     6361 ttcctcgtca ccgccgcatt cgggctgctg gccatcgggg tgcacctcgc ggcggtgagc
     6421 gcggccgcgc cggctgcggc cggcgtgccg ctccttgccg tgttcgccgt cccggcggcc
     6481 ctgaccgacg tcctcctgcc gtggtgggcg atggctgcgg cggccgccgg gttcgggctg
     6541 ctcctggtcg ccgagaccgg gatccggcgc cggatccccg gcggcattgc actcgtggcg
     6601 gtcgcagtgg tgctggcggt cggcgtcgga gcggccgcca gcttcgtcgg aacggcgggc
     6661 cggttcgaca acggcagcgg ttcgggcggg tccggcggct cgatcggtct cagcccgttc
     6721 acggcgctgc ggggccagct cgagcagacg acccccaccg agctgttccg ggtgaagggc
     6781 ctcacccggc ccacctacct ccgggcactg accctgcgtc agtacgtgcc ggacgcaggc
     6841 tggcaagcga cgcggccgga gccaggggtg ggcctgcccg gcccgatcca ggcgcagccc
     6901 ggcatcgccg gtgatctcgc cgacgtggag atcgagaacg tcgccttccg cgactactgg
     6961 ctgccgctct acggggaacc gctcgacgtc ggcgggttgc ccgaggggcc gtggctctac
     7021 gacaagagca gcgacaccgg ctacacgggc cggccgcggc aggagaacgg ttggcgagag
     7081 caggcgttcc tccccgcgcc gaccgtcacg caactgcgac agtccgaagg gtcggccggg
     7141 gtggggccgg agtacctcga tgtcacgggt gtggacccga gggtgggcga gatcgccaag
     7201 caggtcgtgg cgggccggaa caacaacttc gaccgggcga tggcgctgca ggactacttc
     7261 accgggccca actcccagtt cacctacagc ctgcagacgg cgccgggggc gggcgacgat
     7321 gccctcgtcg agttcctcac cgtcggccgg gccggctact gcgagcagtt cgcgtccgcc
     7381 atggccgtga tgctgcggac ggtgggggtc ccggcgcggg tggccgtggg gttcacggcc
     7441 ggtatcgacg acggacagta ccggtcgatc agcacctccg acgcgcatgc ctgggtcgag
     7501 gcatggttcc ccggcgtcgg ctggacgatc ttcgacccca cacccctcac cgacggccgg
     7561 acgatcgcac cgccgtatgt gcaggaagcc cagggcgaca accccggagc tgggaaggct
     7621 cctgccgagc aggatctcct ccccggccag gaagcgggcc aggagccgct acccgcgccc
     7681 gccgaacccg agcagctccc tccgccaccc gtcgccccgt cggcgaccga gggcggcgga
     7741 ctgccgctct ggccgatcgc cgtggtgctg ctcgtggccg tggctgccct ggtgccggcc
     7801 ggcctacggg ctctcgcgag gcggcgccgg atggccgcgg tcggtgcggg cggcccgggt
     7861 gcggcaggag cgggttgggc ggaggtgctc gccgagtccg ctgatcgcgg ggtcacctac
     7921 ccgccgagcg acacggtgcg cggggcggcc cgccgcctcg tgcgcgaaca caagctcgat
     7981 ggcgatgcac agcaggcgct gcggcaggtg gtcggggcgg tggagtcgag ctggtacggc
     8041 gacgtgcacc ccgcccccgg cgagctgcag gagccggtgc gggcggtgcg ggccggcatc
     8101 gcctcggaga gcgcgctgtc actgcgcgga cggctgctac cgaggtcggt ggtccaccgg
     8161 acgccgtcag agcaactcgc acgagcagcc gcacgcgagc ccgccgacca ccgaagctga
     8221 tcggcggacg gcgggtcggt cgtggatggg atggccgggc ggccgggtgt gctattcctg
     8281 ctcgaaccgg cggcggaagc gctcttccat gcggccggtg aagcggttct tgtccggctc
     8341 ggcgtccttg ggctggccgg gctggccacc tgagccgacg gacgtcactg caagcacggc
     8401 tccgccgagc atcaccagga atccgacgac gctcagcacc gggaagctgg acgccagcca
     8461 gagctggggc acggcgacgc cgacgacgag cagcaccagg ccgagaacga acaaaccgac
     8521 gccctgcagc cgtcggcggc gagtgggctt acgcaaacgg ccaccgcgaa cagtggacgc
     8581 gaacttcgga tcctcggcgt agagagcgcg ctcgatctgt tcgagcagcc gctgctcgtg
     8641 ctcggagagg ggcacggctt cacctccggc actggtcatc ctgtcgggtc atcttccatg
     8701 gtgccggaac gacgcgacga gcgcccaggg ctgggcgtct atgggaagga tacgagcctc
     8761 acgcctcgcc gactagccag actcgtcaag cggatgggtc gatcttccgc gatgtgcggc
     8821 gcaggggctc tgagcaggta cgccggtgac gcaacggcgc ccccgtcgcg gcgccgccgc
     8881 gagcgattcg tccatgtttg cgcccgtctg ccccactcgg gatgcccgcg gacaccccgg
     8941 gtgcgcgttc catcacaccc ggttggaccc acccgtcgag ccgcgaacgg tccagagagc
     9001 cgtggcggac ggaccacaag cggcaccgat atctgcgcga tccgcgcaga ccccgcctca
     9061 cccggcgcgg cgggcgagca cgtgcagccg ggcggccatc tccaccagcg gtgcggcaga
     9121 ggcggcgagg tcctccagct ccgcaacggc tcgcgcggcc gccgggccgc cgtcgcgcac
     9181 cgcgctgggt acccacccct cgagcactcc gtcgccctgc agcacctcga cccgcagcgc
     9241 cccgccgtcc tcgagcagtg accgcagggt cgccgcgtcg agccgcctgc gcagcgcgtc
     9301 gtcagtaccg aaccggccgt gtggatcggt gaggacggct cgcgcctcgg cgagcttgcc
     9361 gctgagggtg cgcgccagca cagcggcgaa gcggccgaca acgagcaccg ataccgctcc
     9421 acccgccacc gtggcggtcg tcagcgccga cacggccgcc gcggcgtcat cgacgacctc
     9481 cagcagcccg tgcccgagca cgaggtcggc tccaccggcg gggacgacat cagcgagcgc
     9541 gtcgacatcg ccctgcaggg gcgtgaccag atcgtgcacc ccagcctcgc gcgcccgccg
     9601 gtgtagggcc gcgagcgcgt tggggctgtt gtccacgacg gtgaccgcgc atcccgaccg
     9661 tgcgagcggc accgcccacg ctccgctgcc gccaccgacg tcgagcacgc ggggcggacg
     9721 gtccggccgc acagcgcgca ccctggcaac ctcggcgacc agtacccggt gcaccgagcc
     9781 cccgcccacc gcaccggctg tgtccgccgt cgcaccgctc accatccgag cctcacaacg
     9841 gcagactagt gcgctccggg gaagggtagg ttgatcaccg tgcaggtcgt cgccacgttg
     9901 agcctcaagg gtggggtggg taagacgact gtggcgctcg gattggccgg cgcggcgctg
     9961 caccacgggg tgcgagccct ggtggtggac ctcgatccgc aggccaactc cacgatcgcg
    10021 ctggacgtgg gcaccaccac cgccacggtg gccgacgtgc tcgacgaccc gcgcccgtcg
    10081 gtggtgcgac gcgcgatcgc tcccagcggc tggggcgacg gtctcgacgt gctggtgggc
    10141 gccgagcaga ccgagcggca caaccacccc gaccccggtc cggagcagct gatccggttg
    10201 gcccgggcgc tggagcagct cgacgacgtg gaacaggtcg aggggcgggc gcgccggggt
    10261 gagtccaacg ggcaggcagc ggcgaacggc aaccccggcg acggacacgc ccacgacgag
    10321 ccctaccggc tcgtgatcat cgactgcccg ccgtcgctcg gacagctcac gcgcagcgct
    10381 ctggccgcgg cgcaccgggc tgtgctcgtg acggacccca cgatcttcgc cgtgtcgggc
    10441 gtgcagcgcg cgttcgacgc gatccagacc gagcggcagc gcggcaaccc cgatctgcag
    10501 ccactcggcg tgctcatcaa ccgggtgcgt ccgcgcaaca ccgagcacga gttccggatc
    10561 cacgagctgc gtgagctgtt cggtccgctg gtgttcaccg gggtgcttcc ggaccgctcg
    10621 gcgatccagc aggcgcaagg cgcctgcatg cccatccaac ggtgggacac cccgggagca
    10681 cgcgaggcgt cggctgtgtt cacgctgctc ctgggccggg tgctgcgcag cgagcgaacg
    10741 cagcgtgtgg tcggagcaac gacggcgccg cccgccacgc gctgaccgtc gcaagccgac
    10801 gtcccgtcgt ccggcaggcg agcgccgtca ccgcagcggt accgacgccc cgacgatccc
    10861 cacgaactgc tcggcctgcc gcaacaggtc gtcggcgtcg cggcgtccga ccagccgggc
    10921 gatgccggct tctgccgccg ccctcgtgtc ggagcacgag gcgaagaacg ccgcccactc
    10981 ctgcagctcg ggagcgacct cggcgagcag gtgccagacg ttgcgcgtgg acccgcgccg
    11041 cggttgcggg cgggcgtgga ccgcgagcac ggctgctgcg gcgcggagtg cggccaggta
    11101 ggccgccggg tagcggagca gtggttcttc cgtgcgatgg gcctcgacaa ggccggcggc
    11161 ggcctgccgc agcagcccca gagcggctcg tgaggtcggc gcagccgggc ggaccggtgc
    11221 cctcggatac gggacccggt cagtggtaat ggtcatcgtg gcctcctgga gactctcgtt
    11281 ccgcaacccg caccaggtcc cacacgcctg gtccggcgcg catccgcagc acgaactccc
    11341 gctccggcgc ctccggctcc cccgcggagc gcgcaacaac ccgccagcac cgcagccgat
    11401 cgccaccgac cggcatcggc gacgggaacg gccacggtga ctcctcgatc cagcgctccc
    11461 ggacgtcggt gacgaggtag cgcggccccc gccgaacggg acggcgctgg aactcgatcg
    11521 gctcgccgtc ctgccaccgc acctggatcg gggtgggctg gcgcgatccg aacacccggc
    11581 cgaaggcact catgcgaatt cgctcctccc cgatccgcac cggggcaccc ggtggcgggg
    11641 cgcttccccg ccccaccacc ggagctccgt gatcgaacgc atgttcgatc tgtctccagt
    11701 agaacgctcc ggcacgccct cgtcaagtcg gggttgcacc ggggcggcgc gcaccccgcc
    11761 cgcatggtgt gatggtcggg tgacgagggc cccggcacgc cagctggtcc agctcggtcc
    11821 gggcgatctc gccgaccggc tgacggaggc gctgcacgtg tatgtgagcg ccatgggcta
    11881 ccccaccagc accgcacggc accgtcgcac cctctggctc gaccacaccc gcaggccggg
    11941 ttggcgcgcc gccgggtgga tcgatgcggc cggcaccttg ctcggcatcg cctacggcta
    12001 cacgggcgga cccgggcagt ggtggttcga ggaggtgcgc cgcggcctga gcgctgccgg
    12061 tccggaagcc caggggtgga tccgcgacta cttcgagctc acggagctgc acgtccgccc
    12121 ggatgcgcag gggcatgggc tcggggaggg tctgctgaga gcgctcctcg acggcaccga
    12181 ccgaccgcgg gtgctgctgt ccacacccga gtacggcgca cagcagcccg gccgggcgtg
    12241 gcggctctac cgccgcaccg gcttccgcga cgtcctgcgc aaccatcggt tcaccgggga
    12301 ctcccgcccg ttcgccgtgc tcggtcgcga gctcccgctg gggtccgcgc catagaggcg
    12361 cgccgacagg gctgcccggc accgtcgggg ccggatggca cgatgtccgg cgtgccgacg
    12421 ccgaccgcct cccgccgccg cgtcctcccg ctgctgctgc tcatcctgct cgtggccggc
    12481 acggcgctgg gcgggtgcgc gcgcgtgcgc acggctctcg ccgtgcagcc cgacgactcg
    12541 gtcaccggcg agatgatcct cgccaccccg gagaagggcc ccgacgacac gggaccagcg
    12601 gtcacggtgc caccggagct gaagtccgac gtcgcggtgt cgccgtaccg ccaggacggt
    12661 tacacggggt ccgtgctgcg cttctcgggg ctgaccttcg cggaggtcgc gaccctgagc
    12721 cgggtggcgc ccccggcggc cgatgccgtg cagttcgagc tgcgcagggc cggcaaccgg
    12781 gtgctcgtca gcggtgccgt cgacctcacc accgtgtctg tcgacaaggc cgacttccag
    12841 ctcaagatcg gctttcccgg ccaggtgctg gactccaacg gcgacgccga ctccagcacc
    12901 gtgagctgga cgttcactcc gggccaagtc ggcgacatca acgccgtggt cgcctacgac
    12961 gaccccaacg cgccctctgc ggtgaactgg acgctgctcc tcggcatcgt ggtcgcgctg
    13021 gcggccgggg cggtgctggt cctggcccac cgcacccgca acccgcccgt cagcccgggg
    13081 cgctgacggc gggcgcgggc cgccgggccg ccagcccgag ccgccgcacc aggtcgaccg
    13141 tggccgtgga gcggttcagc gtgtagaagt gcaggccgcg cacgccctcg tcgagcaggc
    13201 gggcgcacat ctcggccgtc acgtccatgc cggctgcgcg gaaggccgct gggtcgtcgg
    13261 agtagggctg gaggcgctcg accagggccg gggggagcgg cgccccggag agctccgggc
    13321 cgcggtgcag ggtgcgcacc gtggtcagcg gcatgatgcc ggggagcagc gggacgtcga
    13381 tgcctgcggc cgccagccgg tcacgcagcc ggaggaagcc ctcgggttcc aggaacagct
    13441 ggctgatcgc gaagtcggcg ccggcccgga tcttcgccag cagccgggca aggtcgaact
    13501 cggcgtccgg tgaccggggg tggccgtagg ggaacgcggc aacgcccacg cagaagtcgc
    13561 cgaggcgacg aaccagcgag accagctcgt ccgcgtaggc gagcccctgg ggatggggga
    13621 cccactcacc cagcgggtcg cccggagggt cgccgcgcac cgcgaggatg ttggagatcc
    13681 cggcggcggc gtaggccccg atcacctggc gcagctcggc caccgagtgc gacaccgcgg
    13741 tgaggtgggc catcggcacg agagtggtgt cc
//
LOCUS       DASRSC010000030        22695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_828120,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000030 DASRSC010000000
VERSION     DASRSC010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 22695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22695
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828120"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFE39_01360"
     CDS             complement(<1..205)
                     /locus_tag="VFE39_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257275.1"
                     /translation="MTAAPRATRHDTTVPPTVRLSVLLWLAAIAAGAVEALVWLALPD
                     PPTPTQLGLRFSIYTVLTVLVLAL"
     gene            complement(281..1360)
                     /locus_tag="VFE39_01365"
     CDS             complement(281..1360)
                     /locus_tag="VFE39_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET6257276.1"
                     /translation="MAQDETNPGDASGGAGGESTLTVLVATAVNALIAVLKFVAGALT
                     GSAAMFAEAAHSVADTATELLLLTALRQSARRPDRRHPFGYGKARFFWALIAAVSIFV
                     SGAVFAFFEGIRAMLGSDAEQTLAWVAYAVLGVSFLLESISWLQAVRQVRRESAREDV
                     GFRAWLHTTDDPTVKSVFFEDSAALIGLLLAFAGVGLHQLTGSGFWDGLASVLIGALL
                     TGVAFILGRTNAGLLIGRQADLRTVYAFRDALAARPEVDQVVDLLTMTVGTDEVLLCA
                     RLDFDDALDVAALERACVEIDADLHARFPDLQEVFLEPVPRADPTMRQRVLERYGGAG
                     AQRLRDGLGQDRGRGVGDGWGAPPS"
     gene            complement(1388..2215)
                     /locus_tag="VFE39_01370"
     CDS             complement(1388..2215)
                     /locus_tag="VFE39_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6257277.1"
                     /translation="MSDRTFRFGAVATPTGGPDEWTGTARRIEELGYSSLLMPDGLQL
                     LAPFPSLAVAASATKALRVGTFVLASPLRPPLAAAWEGHSLSVLTGGRFEFGIGTGRP
                     AVREFAERLGLPFGTAGERLQQVAESIDHLRELDGAERHTPVLVAAGGPRALAVAAEK
                     ADIVTLAAAPLAPRDEVAGMARTLRDAAGDRADDIELAMNLFVVGSEVPPWTREFIGA
                     DAATLIAHDSLVMLRGSTTEMVDELQRRRETLGVSYVSVNSAFVDALAPVVERLTGR"
     gene            2298..2774
                     /locus_tag="VFE39_01375"
     CDS             2298..2774
                     /locus_tag="VFE39_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6257278.1"
                     /translation="MAASARRLEDPPGSAFLLAALGAHAAARFAERIAALDVTPAQTG
                     LLRAIAATPGQSQQVLAQLLGTPPSRLVGLVDSLADRGLVERRRNPTDRRLHALHLTA
                     DGATLVERIETIGREHDDAILRSLKDSERARLRSLLARIAEDQGLTPGVHPSYRRL"
     gene            2907..3146
                     /locus_tag="VFE39_01380"
     CDS             2907..3146
                     /locus_tag="VFE39_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257279.1"
                     /translation="MATDSENGCSPVPDTAMGTGNDEGEPGERLGDGTLDPEEQAVLD
                     ELRQGGGDVVVSEAEEDAPAVERAVEDDTPVPGGG"
     gene            3252..4013
                     /locus_tag="VFE39_01385"
     CDS             3252..4013
                     /locus_tag="VFE39_01385"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HET6257280.1"
                     /translation="MTPTTPVLADRTALVTGAASGIGAAIVRRLVDDGAAVLAVDRDE
                     AGLKALAAAVPRVEALLCDLADLDAVDKLPAAVDVLVNNAGLQHVAPVPEFDPERFSL
                     ILRVMLEAPFRLARRTLPHMYERGWGRVVNISSVHGLRASPFKSAYVSAKHGLEGLSK
                     VIALEGAGRGVTSNCINPAYVRTPLVERQIADQARTHGIDEDAVLEQVMLGPVAVKRL
                     VEPDEVAELAAVLYGPASASISGSSLSMDGGWTAH"
     gene            4032..5372
                     /locus_tag="VFE39_01390"
     CDS             4032..5372
                     /locus_tag="VFE39_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6257281.1"
                     /translation="MVDKPQPRSNIITVVAASMAGTTVEWYDFFLYGVAAAVVFPAVF
                     FPSSDPAVGTLLALGTFAIGFIARPIGGLVFGHYGDKIGRKTLLVVSLVMMGVATFAI
                     GLLPGYATIGLAAPLLLVLLRLIQGFALGGEWGGAVLIVSEHGDAKRRGYWASWPQAG
                     VPFGQLLANGLLFLLSAVQSQDDFNSWGWRIPFLLSAVLVLIGLYVRLSVEESPVFKA
                     AQARAAEQAAAGEKQTMPIIEVFRRYPREVFTAMGARFAENVAYYIFTIVITTYVTKR
                     LGLPSSFVLGAVLIGAAFHLVTIPIWGALSDRFGRKPIYLFGAAGVGIWAFVFIALVD
                     TRNFGLTVLAVIGGLLFHGAMYGPQAAFLSELFGTKVRYSGVSIGYQLASVVAGGLAP
                     VIAVALYTAFDSGYAVSVYVAISAAVTILAVATYGETRDRDLAEDPALPRSAVR"
     gene            5405..7258
                     /locus_tag="VFE39_01395"
     CDS             5405..7258
                     /locus_tag="VFE39_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6257282.1"
                     /translation="MSETSLELLRLLAQDAPAERIDEQARALAAVDPQSGAVARELAL
                     RVRSGIDAHRRREAELSALVDIARDLASRPDPGGVLDAIVRRARSLLSTDVAYLTLAD
                     AERGDTFMRATAGSVSARFQALRLPLGAGLGGLVAQTRRPYWTADYPGDERYAHTYEI
                     DAAVDEEGLVAICGTPLLVDGEFVGVLFAANRTRRPFSRSEVALLGSLAALAAVSLVQ
                     SRRAAETAEALAALSSAHAGIEQAAAAHDRFAGVVLSGGGVDDIAAALGELLGCWVAV
                     LDVDDQRIATHGPAPDPGTDPDPLAAAVAVRRSAASGRLAEADGLSAAVVSAAGQRLG
                     TLVLGGRQPLDSGQGRTVERAAMVTALVLLFRLRAAEADQRVRTDLLADLLARPDGQV
                     DGALVERGRLLGLRLQTPHVVAVCRGARRGVHLAAATAGDGRGLAGDHNGEVVAVVPG
                     RDPSAIAAAMARRLSADDVQVTVGATGPVLPAAGVRDAHAEARRVTDALVAMGISAGS
                     ARDLGFAGLVAGGPADVRRYLDRILGPVAEYDTRRGSDLLGTLEAYFAAGNSPRHAAG
                     ALHVHVNTVAQRLERVASLLGEDWHCPDRALEIQLALRLRKLQAGQAGLDG"
     gene            complement(7255..8079)
                     /locus_tag="VFE39_01400"
     CDS             complement(7255..8079)
                     /locus_tag="VFE39_01400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6257283.1"
                     /translation="MTVDREKITLTGAQETLLATLYGRALDSRSPRSILHDDGAEQAV
                     RRIDYDFRKAGVNATSAAGIALRAKQLDSWTAEFLADHQEATVLHLACGLDTRVQRLD
                     HPPSVRWFDVDHSEVIALRARLLPAPPGDYRMIGTSVTADAWLDAVPDDRPIVAVFEG
                     LSMYLRKEDGTRLIERICGRFASGQLLFDCYGSLGIRLQKLVPAVRNAGATLHWAIDD
                     PREIEGWHDGLQCLDALRSVDMPGLDQMPASGRTSMWVLAHLPGFRDVGRILRYRF"
     gene            complement(8123..9229)
                     /locus_tag="VFE39_01405"
     CDS             complement(8123..9229)
                     /locus_tag="VFE39_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6257284.1"
                     /translation="MSDRASAPAAATRLVEQVRERATAGEAPRRRAGWRSAVAPVAVL
                     VVLAALPYASVPLPGVLPGLVNSPGSMQLLALCLVFGGVALSYDLLFGRTGLLSFGHA
                     LFVAAGSYTAALALARPGVGLAAAVGAAVVVGVLLPLLVGAVALRVTGIAFAMVTLAF
                     AQAGSILVLRNPGGLTGGEEGLPLNREGLPNLLVGVVNAPYRYWLALAYLALCWAVVA
                     WLVRSRVGHIWAAARENPERVAVLGLSVYRAQLLAVLVGGLLGTLGGVAHLLVVGGAT
                     PSLTTSELTLGLLVMVVLGGAGSRWGAVLGGVLYTYLDNRLLELAGSHAVAGLPAVLR
                     VPLSEPTFVLGALFVAVVFFAPGGLVGLLRRRIR"
     gene            complement(9226..10116)
                     /locus_tag="VFE39_01410"
     CDS             complement(9226..10116)
                     /locus_tag="VFE39_01410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6257285.1"
                     /translation="MSTFVLLLATGLGLGALYFLVASGLSLIYGLMGVLNFAHGAFLA
                     AGAYAAWWAAAGGLPGAGGDGWGFVLATAFGVAVGAVIGASVELLLIRPLYRRHIEQV
                     LVTVGLGLVGVALLRGIWGADPLPFPRPEVAAGVTTIGGAAIPNDRFVLLVAAIAVLL
                     GLLALLRFTRIGLIIRAGVENRSMVEALGIDVRRTFTLVFALGGAVAALGGVLGSVYL
                     GSVSPGQGTSLLIFAFIVVVIGGLGSLTGSAVAAVVVGLVQQLANYYTASGVGDLSVV
                     LLLALVLLVRPGGLSGRAAA"
     gene            complement(10181..10900)
                     /locus_tag="VFE39_01415"
     CDS             complement(10181..10900)
                     /locus_tag="VFE39_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6257286.1"
                     /translation="MRAISTEPVLELADVHVRIGGSHVLQGVSLAVAPTGVTALLGRN
                     GVGKTTTLRAVVGLVPRTGSIRLAGDEIGRGRTPWVVRQGVGYVPEDREVFATLTVAE
                     NLRLACPDPEPERAALVHELFPELAERSRQRAGTLSGGQQQMVALARALLPPSRLLLI
                     DEPTKGLAPRLVTEVAAALERVAQVTPVLLVEQNLPVVRRLAGDVVVLDAGRVVHAGR
                     ADELLADAGRTRELLGVGSKS"
     gene            complement(10897..11661)
                     /locus_tag="VFE39_01420"
     CDS             complement(10897..11661)
                     /locus_tag="VFE39_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6257287.1"
                     /translation="MSATATAAPVLAARDLGWVVGGAVILADVHLDVRDGELLAVIGP
                     NGAGKSTLLHLLSGVRRPTSGTVELGGRDVTRASAAARARHGLGRTFQTSSLFGGLSA
                     RENVRLAVQSRRARPLSVLRGAQGREELAEADALLEQVRMAHRATATAGALAHGDKRK
                     LEIAIALARRPSVLLLDEPMAGVSVEDVPPLVELIGGLRAAGVSVGMVEHHMDVVLGL
                     ADRVAVLHHGRLLAVGSPAEVTADATVQQAYLGEEL"
     gene            complement(11667..12875)
                     /locus_tag="VFE39_01425"
     CDS             complement(11667..12875)
                     /locus_tag="VFE39_01425"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6257288.1"
                     /translation="MLQRSGRSPAALLIAVLAVLLAGCGAPGSGGSDAGGNSATAPFR
                     VGLVHSTTGPLASYGKQYSAGFRAGLDYATKGTGQVNGRQVQITEADDAGDPAKAVAA
                     ATDLIGQGYKVIAGSTASGVALQVAPLAEENKVLFVSGPAATDGITGVNKYTFRSGRQ
                     TYQDVLTAGSIIGDPAGRSVTVFAQDSAFGQANAQAVTAVLGAKGARVTPVLVPAGAT
                     DLTPFARQAVDAKPNLLFVAWAGANATQMWQSLQQQGAFEASTVVTGLDIRSTYPIFG
                     AAADKIQFLSHFFPGATDNEAYKALEAGLAKQGAEVDLFTNDGFVAAQMVVHALQEGG
                     DDTAAMVKALEGWAFTGPKGESTIRAQDHALLQPMFTARLTQTASGFAPQLVATLPPD
                     AVAPPVAPAK"
     gene            complement(13200..13640)
                     /locus_tag="VFE39_01430"
     CDS             complement(13200..13640)
                     /locus_tag="VFE39_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET6257289.1"
                     /translation="MSSARYVRATRGTDVFNDIVAWLTRMGLSIWGSRLLAVRGRKSG
                     EWRTVPVNLLAYDGVRYLVAPRGHTQWVRNLRVAGGGELRVGRRVEEFTATELADDDK
                     PAVLREYLRRWKFEVGVFFDGVDASATDEQLRAMAPGYPVFRLV"
     gene            complement(13754..14011)
                     /locus_tag="VFE39_01435"
     CDS             complement(13754..14011)
                     /locus_tag="VFE39_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257290.1"
                     /translation="MREQRWVAHVGEDNASWLSTESRTARLASEYRPRDVGAGRIELS
                     SLALGASRELGEEEDGYITDDGDGIRVWIADDAFELLLVED"
     gene            complement(14030..14665)
                     /locus_tag="VFE39_01440"
     CDS             complement(14030..14665)
                     /locus_tag="VFE39_01440"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET6257291.1"
                     /translation="MPEVLAVCDAVLYSPWLLPVLVVLIALDGPFPVLPSETLLMTAA
                     AVAFGSHDVTGVVGLFVAALVGSAAGDLLVFGLGRSSHRVLAKTIDVECGLSSWVRRH
                     LLPRPGIALVGARFVPGGRLVSTAAAGRYGLGLHRFLFWSLVSSGAWSLYMLLIGLLL
                     GPITGGSPVLSLLAGLVMAVLTALAFAIGRRVQVWRAVRRPEPLAELVTPR"
     gene            14913..15737
                     /locus_tag="VFE39_01445"
     CDS             14913..15737
                     /locus_tag="VFE39_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET6257292.1"
                     /translation="MLHAVAALVAAAVLGVAGPASAAAAGPAVPGQGSSGEPISPGIL
                     MLSPVSESAANACTASFVFKSGGTTYLGYAAHCAGTSLTGLSGCREKTLPLGSTVLIE
                     RGNGASSTGRLAYNSWATMQRRGETDQSLCMFNDFALVALDPSDAGEVDPSVPILGGP
                     TALDTDGLRSGEPVFSYQPNNGGTVVKEGKTVSDSGGGRSHRVNTTPPGRPGDSGSGY
                     LDGNGMAFGVLSTQFLDGTGTNGLTDLAMALTYANQYGGIGRVALVPGTEPFAPPG"
     gene            complement(16204..17820)
                     /locus_tag="VFE39_01450"
     CDS             complement(16204..17820)
                     /locus_tag="VFE39_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET6257293.1"
                     /translation="MSETARTVRDATFDVLRRHGLTTIFANPGSTEVAFLTDLPDDVD
                     FVLALHEGSVVGIATGWATALDQPAFVVLHTTAGFGNAVGALATARVNRVPLVVLVGQ
                     QDRRHLALQPFLAGELEGLAGRYPVWVTTPPRPQDVPGAVARAWHEARHGRGPAVVIV
                     PSDDWAAPVDDTPLGAPAVLYRASAVDPAAVEALADMISRSESPALVVGADADDAETW
                     AALVELAERLRCPVWQESFAARAGFPQDHPQFWGHLPPGRARLRQALAGHDVVLAVGA
                     PVFRQYPFEPGPLVEDGTKVALVTDDVEDAHHSPVDLAVVARPAPVCRQLAARVPVRD
                     DEPVSPPDPIPPPRRPRAGEPLRAAHVMVALAERIPVETVVVEETPSTRPDLHRLVPA
                     RRPMGFLSAAMGGLGFAMPAAIGVRMARPDDPVVAVVGDGSSLYAIQSLWSAAHYGCG
                     VLFVVLANGRYAIMDRLAEQHGSGKAPWPAFEEVSVHRLAQALGCPAERVDSYDGLVA
                     ALDAVVPTLAGRSEPLLLEVAVEPEPTFEA"
     gene            complement(17817..19085)
                     /locus_tag="VFE39_01455"
     CDS             complement(17817..19085)
                     /locus_tag="VFE39_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid/H+ symport family MFS transporter"
                     /protein_id="HET6257294.1"
                     /translation="MSTDSPVNPQNPREQYAGWVAPLCWSAVALEGFDLVVLGVVLPP
                     LLRYPDWGLTPGTASLISVVGLLGVMIGALAIGPVTDIIGRRRTMLLTVVGFSLLTLL
                     CAFAPNPWIFGLLRFLAGLGLGGVLPVALALVNEYAREGRSGSATTTLMTGYHVGAVV
                     TALLGIVVIPAFGWRPMFVLGALPALVLVPLLLRYLPESTSYLRERALGATPGSTGPE
                     RNPVTILFRGGYLRATLAFWVTSFMGLLLVYGLNTWLPEIMRSAGYALGAALALLLVL
                     NLGAVLGLLVAGQVANRFGTRRSTIAWFTAAALFLALLSVRLPGIGVYVSVLMAGVFV
                     FSAQVLVYAYVSQLYPVSARGTALGAASGVGRLGAISGPLIGGALLSAGIAYPWGFYI
                     FATVAAIGAVSVALVARTAEPAAVERTTAG"
     gene            19245..19649
                     /locus_tag="VFE39_01460"
     CDS             19245..19649
                     /locus_tag="VFE39_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET6257295.1"
                     /translation="MTSTTTSTNRVTNRVLWGIQILVGIFFIVASAAPKFFGEAYAVE
                     IFTQIGAGQWFRYLVAVLELAGGIGLMTTRLAAPAALGLMGLMIGAAFTQAVVLDAPA
                     MVLTPAILFVVLAAIAWGRRAQITAQLPSQAG"
     gene            complement(19656..20441)
                     /locus_tag="VFE39_01465"
     CDS             complement(19656..20441)
                     /locus_tag="VFE39_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6257296.1"
                     /translation="MSGGMRELDGRRAVVTGGTRGGIGAAVTARLTDAGARVLTTARS
                     TPDDVVHPELFVASDVGTAEGVARVAEAALNRLGGVDIVVHVVGGSRQEPGGVLALSD
                     DDWQDAFAVNLFASVRLDRALLPSMIDQGRGAIVHVTSIQRRVPLPTTVPYAAAKAAL
                     TSYSKNLATQMAPKGVRVNTVAPGFVETAGARGMVSALAAADGVDENTSRRRIMDSIG
                     GIPLGRPARPEEVAELIAFLVSDRASAITGAEHVIDGGSLRTI"
     gene            complement(20438..20767)
                     /locus_tag="VFE39_01470"
     CDS             complement(20438..20767)
                     /locus_tag="VFE39_01470"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6257297.1"
                     /translation="MSTNLPAPVAAYLEAKDDDHVEAVLESFTDDAIVHDEGKDHVGI
                     AAIRDWAAAISTTYQLERTVLETRRTGAATVMLVRVAGNFPGSPVDLHHHFTDSGDRI
                     AALTIWP"
     gene            complement(20828..21676)
                     /locus_tag="VFE39_01475"
     CDS             complement(20828..21676)
                     /locus_tag="VFE39_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6257298.1"
                     /translation="MDRRELADFLRRRREQLRPADVGLAAGMRRRTPGLRREEVAQLA
                     GVSADYCMRLEQQRSSQPSTQVLAALARALRLTADERDHLYVLAGHPAPAGRLAGDHV
                     RPGLMHLLDALPATPAQILGDLGDLLAQNEMARYLFGAVCSEREESRNVVVRWFTDPG
                     VRAAHPPEEHEYLSRVHVADLRAAVARRGNDAAAAGLVRRLRATTAEFAELWDRHEVA
                     VRRRSRMRVLHPRIGLIELDSETLLTPSEDQKLLVFTPPPGSETAEKLGLLRVIGDQD
                     LTTTGR"
     gene            21807..>22695
                     /locus_tag="VFE39_01480"
     CDS             21807..>22695
                     /locus_tag="VFE39_01480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CE1758 family FMN-dependent luciferase-like
                     monooxygenase"
                     /protein_id="HET6257299.1"
                     /translation="MQLGIFSVGDVTPDPTNGRTPTEAERIKAMVAIALKAEEVGLDV
                     FATGEHHNPPFVPSSPTTMLGFIAARTERLQLSTATTLITTNDPVKIAEDYAMLQHLA
                     DGRVDLMMGRGNTGPVYPWFGQDIRNGIALAVENYALLRRLWREDVVDWQGKLRTPLQ
                     SFTSTPRPLDGVPPFVWHASIRSPEIAEQAAFYGDGFFHNNIFWNKEHVQRMVELYRR
                     RFEHYGHGAADQAIVGLGGQVFIRKNSQDAVREFRPYFDNAPVYGHGPSLEEFTDLTA
                     LTVGSPQQVIEKTLSFREMA"
BASE COUNT         3441 a         8293 c         7967 g         2994 t
ORIGIN      
        1 gcagggccag caccagcacg gtgaggacgg tgtagatcga gaaccggagc ccgagctgcg
       61 tcggagtggg cgggtcgggc agcgccagcc agacgagggc ctcgaccgcc cctgcggcga
      121 tggcggcgag ccagagcagg accgagagcc ggaccgtagg gggaacggtg gtgtcgtgcc
      181 tcgtggcgcg cggggcagcg gtcacggtgt acctcctcgg tggacgtgcc accgagtctt
      241 tgccacagcc gctgaagagt cgggccggtg cgcccccgga tcaggatggg ggcgcgcccc
      301 acccgtcgcc gacgccccgc cctcggtcct gccccagccc gtcccgcagc cgctgtgccc
      361 ccgctccccc gtagcgctcg agcacgcgct gccgcatggt cggatcggcg cgcggtaccg
      421 gctcgaggaa gacctcctgc aggtcgggga agcgggcgtg caggtcggcg tcgatctcca
      481 cgcaggcccg ttccaacgcc gccacgtcga gcgcgtcgtc gaagtccagg cgggcgcaga
      541 gcagcacctc gtcggtgccc acggtcatcg tgagcaggtc gaccacctgg tcgacctcgg
      601 gccgggcggc gagcgcatca cggaacgcgt agacggtgcg caggtcggcc tgccggccga
      661 tgagcagccc ggcgttcgtc cgtccgagga tgaacgccac gcccgtgagc agtgcaccga
      721 tgagcacgga ggccagaccg tcccagaacc ccgagccggt gagctggtgc agtcccacgc
      781 cggcgaaggc gagcaggagg ccgatgagcg ccgcactgtc ctcgaagaag acgctcttca
      841 ccgtcgggtc gtcggtggtg tgcagccagg cccggaagcc gacgtcctcg cgcgcggact
      901 ccctgcgcac ctggcgcacc gcctgcagcc aggagatgga ctcgagcagg aacgacacgc
      961 cgagcacggc ataggccacc cacgccaggg tctgctcggc gtcgctgccg agcatcgcgc
     1021 ggatcccctc gaagaacgcg aacacggccc cggagacgaa gatgctcacg gccgcgatca
     1081 gcgcccagaa gaagcgggcc ttgccgtagc cgaaggggtg gcgtcgatcc ggccgccggg
     1141 ccgactgccg caacgcggtg agcagcaaca gctccgtggc ggtgtccgcg acgctgtgcg
     1201 ctgcctcggc gaacatcgcg gccgacccgg tgagcgcccc cgccacgaac ttcagtaccg
     1261 cgatgagggc gttgaccgcc gtcgcgacca ggacggtgag cgtgctctca cccccagcac
     1321 ccccgctcgc gtctcccggg tttgtctcgt cctgggccac ggggacgagg ctaggcggcc
     1381 ggtgggatca gcgccctgtg agcctctcga ccaccggggc gagggcgtcg acgaaggccg
     1441 agttgacgct gacgtaggac acgccgaggg tttcgcggcg ccgctgcagc tcgtcgacca
     1501 tctcggtggt actgccgcgg agcatcacca gcgagtcgtg cgcgatgagc gtggccgcgt
     1561 cggcgccgat gaactccctg gtccacggcg gaacctcgct acccacgacg aagaggttca
     1621 tcgccagctc gatgtcgtcg gcacggtcac ccgccgcgtc ccgcagcgtc cgcgccatgc
     1681 ccgccacctc gtcccgcggg gccagcggtg cggcggccag cgtgacgatg tcggccttct
     1741 ccgcggcgac cgcgagcgcc ctcgggccgc ccgcggcgac cagcaccggg gtgtgccgct
     1801 cggcgccgtc gagctcgcgc aggtggtcga tgctctcggc cacctgctgg agccgctccc
     1861 ctgccgtgcc gaacgggagg ccgaggcgct ccgcgaactc ccgcacggca ggacggccgg
     1921 tcccgatacc gaactcgaag cggccgccgg tgaggaccga gaggctgtga ccctcccacg
     1981 ctgcggccag gggtgggcgc agcgggctcg ccagcacgaa ggtgccgacg cggagagcct
     2041 tcgtggcgct cgccgccacg gcgagcgagg ggaacggcgc cagcaactgc aagccgtcgg
     2101 gcatcaggag ggaggagtag cccagctcct cgatccgccg ggcggtgccg gtccactcgt
     2161 ccgggccacc ggtgggcgtg gcaacggccc cgaaccggaa cgttctgtcg ctcatctgtc
     2221 cctcctcatc ccaataacga ttacaatcct gaacggttca ccacttgaca tgatttgtca
     2281 actgatacgg tcttccgatg gctgcgtccg cccgacgtct cgaggaccct cccgggtccg
     2341 ccttcctgct cgccgcgctc ggcgcgcacg ccgcagcccg cttcgccgag cggatcgcag
     2401 cgctggacgt gacgcccgcg cagaccggtc tgctgcgggc gatcgccgcc accccgggcc
     2461 agagccagca ggtgttggcg cagctgctgg gcactccacc gagccggctg gtgggactgg
     2521 tcgactccct cgccgaccgc ggactcgtcg agcgccgccg caacccgacc gaccggcggc
     2581 tgcacgccct gcacctgacc gccgacggcg caacgctcgt cgagcggatc gagaccatcg
     2641 gccgggaaca cgacgacgcg atcctccgca gcctgaagga ctcggagcgg gcccggctgc
     2701 gctccctgct cgcccgcatc gccgaggacc agggcctcac accgggcgtg cacccgagct
     2761 accggcgcct gtgagcggtc cgaccccggg catgatcatc gtcctggcgc ggaagcaacc
     2821 ggatccggcc ggtatctgcg ccgaggcggt gatcgtggtg tgtggcgtcg gttcaaggcc
     2881 cgccacagcg gggtaccggc cccggtatgg caacggattc cgagaacggg tgtagtcccg
     2941 tccctgacac ggcgatgggg accggcaacg acgagggcga gccgggcgag cgcctcggcg
     3001 acggcacgct cgaccccgag gagcaggcgg tgctcgatga gctccgtcaa ggcggcggcg
     3061 acgtggtggt gagcgaggcg gaggaggacg cacccgcggt ggagcgagca gtcgaggacg
     3121 acacgccggt gcccggcggg ggctgactcc cggtcgcagc gggatgtgtg tcccaccaac
     3181 acatcgggcg acgcatccgt ggtgtctccg caccgcgcgg atccgacgct acggacgtac
     3241 cgtcctccgg catgacccca accacgccgg tgctggccga ccgcaccgcg ctcgtcaccg
     3301 gtgctgcgtc gggcatcggc gccgcgatcg tgcggcggct ggtggacgac ggtgcggcgg
     3361 tgctcgcggt cgaccgtgac gaggccggtc tgaaggcact cgccgccgcc gtgccccgcg
     3421 tcgaagccct gctctgcgac ctcgccgacc tcgatgcggt cgacaagctg cccgccgccg
     3481 tcgacgtcct ggtcaacaac gccggcctgc agcacgtggc cccggtgccc gaattcgacc
     3541 ccgagcgctt ctcgctgatc ctgcgcgtga tgctggaagc accgttccgg ctggcccggc
     3601 gcacgttgcc gcacatgtat gagcgcggct ggggccgggt cgtcaacatc tccagcgtgc
     3661 acggcctgcg cgccagcccg ttcaagtccg cctacgtcag cgcgaagcac ggcctggaag
     3721 ggctgtccaa ggtgatcgcg ctggagggcg caggacgcgg cgtcaccagc aactgcatca
     3781 accccgccta cgtccgcacg ccgctcgtcg agcggcagat cgcggaccag gcccgaacgc
     3841 acggaatcga cgaggacgcc gtgctcgagc aggtgatgct cggcccggtg gccgtgaaac
     3901 ggctcgtcga acccgacgaa gtggccgagc tcgccgcggt tctctatggg cccgcctcgg
     3961 cgtccatctc cggatcatcg ctgtcgatgg acggcggctg gactgcccac taggaacaac
     4021 cgggagacaa cgtggtcgac aaacctcagc ctcgcagcaa catcatcacg gtggtcgcgg
     4081 ccagcatggc cggtaccacc gtggagtggt acgacttctt cctctacggc gtggccgcgg
     4141 ccgtcgtctt ccctgcggtg ttcttcccgt cgagcgaccc cgccgtgggc acgctgctcg
     4201 cgctgggcac gttcgcgatc gggttcatcg cacggccgat cggcgggctg gtcttcggcc
     4261 actacggcga caagatcggc cgcaagacgc tcctggtggt cagcctcgtg atgatggggg
     4321 tcgccacctt cgcgatcggg ctgcttccgg ggtacgcgac gatcgggctg gcggcaccgc
     4381 tcctcctggt gctcctgcgt ctcatacagg gcttcgcgct cggcggtgag tggggcgggg
     4441 cggtgctcat cgtctccgag cacggcgatg ccaagcggcg cggctactgg gcgagctggc
     4501 cgcaggccgg ggttccgttc ggtcagctcc tcgccaatgg cctgctgttc ctgctgtcgg
     4561 cggtgcagag ccaggacgac ttcaactcgt ggggctggcg gatcccgttc ctgctctccg
     4621 cggtgctggt gctgatcggg ctctacgtgc ggctctcggt ggaggagtcg ccggtgttca
     4681 aggcggccca ggcccgcgct gcggagcagg ccgcggccgg ggagaagcag acgatgccga
     4741 tcatcgaggt cttccgccgc tatccgcgcg aagtgttcac cgcgatgggt gcgcggttcg
     4801 ccgagaacgt cgcctactac atcttcacca tcgtcatcac gacgtacgtg accaagcggc
     4861 tcggcctgcc gagctcgttc gtcctcgggg ccgtgctgat cggcgcggcc ttccacctgg
     4921 tgacgatccc gatctggggg gcgctgtcgg accgcttcgg gcgcaagccg atctacctgt
     4981 tcggggccgc gggcgtcggt atctgggcgt tcgtgttcat cgcgctcgtc gacacccgca
     5041 acttcgggct caccgtgctc gccgtgatcg gtggcctcct cttccacgga gcgatgtacg
     5101 ggccgcaggc cgcgttcctg tccgagctgt tcggtacgaa ggtgcgctac tccggcgtct
     5161 cgatcggcta ccagctcgcg tcggtcgtcg cgggcgggct ggctccggtg atcgcggtgg
     5221 ccctgtacac cgcgttcgac agcgggtacg cggtctcggt gtacgtggcg atcagcgccg
     5281 cggtgacgat cctcgcggtc gccacctacg gcgagacgcg ggaccgtgac ctcgccgagg
     5341 accccgcact cccacgcagc gcggtccgct gagcacccac cgtggcggtg gcatgatcgc
     5401 cgccgtgtcc gaaacctcgc tggagctcct gcgcctgctg gcgcaggacg ctcccgcaga
     5461 gcggatcgac gagcaggcgc gggcgctcgc agcggtcgac ccgcagagcg gggcggtggc
     5521 tcgggagctc gcgctgcggg tgcgcagtgg catcgacgca caccgcagac gggaggcgga
     5581 gctgtccgcg ctcgtcgaca tcgcccggga cctggcgtcc cggcccgacc ccggcggggt
     5641 gctcgacgcg atcgtgcgcc gcgcccgcag cctgctcagc actgacgtcg cctacctgac
     5701 actggccgac gccgagcgtg gcgacacgtt catgcgggcg acggccggct cggtgtcggc
     5761 acggttccag gcgttgcggt tgcccctggg cgccgggctc ggcgggctgg tggcgcagac
     5821 ccggcggccg tactggaccg ccgactaccc gggcgacgag cgctacgcgc acacgtacga
     5881 gatcgacgcc gcggtggacg aggaggggct ggtcgcgatc tgcgggacgc cgctgttggt
     5941 ggacggcgag ttcgtcgggg tgctgttcgc cgccaaccgc acccggcggc cgttcagccg
     6001 cagcgaggtg gcgctgctcg gctcgctggc cgcgctcgcc gccgtctcgc tcgtgcagtc
     6061 caggcgcgcc gccgagaccg ccgaggcgct ggccgcgctg tcgagcgcgc acgccgggat
     6121 cgagcaggcc gcggcggcgc acgaccggtt cgccggcgtc gtcctgtccg gaggcggggt
     6181 ggacgacatc gccgccgccc tcggcgaact gctcgggtgc tgggtggcgg tgctcgacgt
     6241 cgacgaccag cggatcgcaa cgcacggacc cgcccccgac ccgggaaccg acccggaccc
     6301 gctcgcggcg gcggtcgccg tccggcggtc cgcggcgtcg ggccggctcg cggaggccga
     6361 cgggctgtcg gcggcggtgg tgagcgcggc cgggcagcgg ctgggcacgc tcgtgctcgg
     6421 cggccgacaa ccgctggaca gcgggcaggg ccgcaccgtg gagcgggcgg cgatggtgac
     6481 cgcgctcgtg ctgctgttcc gcctccgtgc tgcggaggcc gaccagcggg tgcgcaccga
     6541 cctgctcgcc gacctcctgg cccgcccgga cggccaggtc gacggcgcgc tcgtcgagcg
     6601 cgggcggctg ctcgggctgc ggctgcagac cccgcacgtg gtggcggtgt gccgtggcgc
     6661 gcggcggggc gtgcacctgg ccgcggccac cgcaggggac ggccgcgggc tcgccgggga
     6721 ccacaacggc gaggtcgtcg ccgtggtacc cgggcgtgac ccttcggcca tcgccgcagc
     6781 catggcgcgt cggctgagcg ccgacgacgt tcaggtcacc gtcggtgcga ctggcccggt
     6841 cctacccgct gccggcgtgc gcgacgccca cgccgaagcc cgccgcgtca cagacgcgct
     6901 ggtggcgatg gggatatctg ccgggtcggc ccgcgacctg ggtttcgccg gcctggtggc
     6961 gggcggaccc gccgatgtcc gccgctacct cgaccggatc ctcggcccgg ttgcggagta
     7021 cgacacgcgc cgcgggagcg acctgctcgg cacgctggag gcctacttcg cggcaggcaa
     7081 cagcccgcgg cacgcggcgg gcgcgctgca cgtccacgtc aacacggtgg cgcagcgact
     7141 cgagcgggtt gcgtcgctcc ttggcgaaga ctggcactgc ccggaccggg cgctggagat
     7201 ccagctggcc ctgcggctgc ggaagctgca agccggccag gccggcctgg atggctagaa
     7261 ccggtagcgc aggatccggc cgacgtcccg gaagccgggg aggtgcgcca gcacccacat
     7321 gctcgtccgg cccgaggccg gcatctggtc caggcccggc atgtcgacgc tgcgcagcgc
     7381 gtcgaggcac tgcagcccgt cgtgccagcc ctcgatctcg cgcgggtcgt cgatagccca
     7441 gtgcagggtg gcgccggcgt tgcgcaccgc gggcacgagc ttctgcagcc ggatgcccag
     7501 cgagccgtag cagtcgaaga gcagctggcc gctggcgaac cggccgcaga tccgctcgat
     7561 cagccgcgtg ccgtcctcct tgcgcaggta catcgacagg ccctcgaaga cggccacgat
     7621 cggacggtcg tcgggcacgg cgtcgagcca tgcgtcggcg gtcaccgacg taccgatcat
     7681 tcggtagtcc ccggggggtg ccggcagcag gcgcgcgcgg agggcgatga cctcggagtg
     7741 gtcgacgtcg aaccagcgaa ccgacggcgg gtggtcgagc cgctggacgc gcgtgtccag
     7801 gccgcaggcg aggtgcagca ccgtggcctc ctggtggtcg gccaggaact cggcggtcca
     7861 gctgtcgagc tgcttggcgc ggagcgcgat cccggccgcg ctcgtggcgt tcaccccggc
     7921 cttgcggaag tcgtagtcga tccggcgcac tgcctgctcg gccccgtcgt cgtgcaggat
     7981 ggaccgcggc gatcgactgt cgagcgccct gccgtacagc gtggcgagca gagtttcctg
     8041 cgcgccggtg agggtgatct tctcgcggtc gacggtcacc agcagaggat ccggcagcac
     8101 caccgcgccg tccaaagcgg gatcaccgaa tccgccgccg cagcagcccg accaggccgc
     8161 cgggggcgaa gaacaccacc gcgacgaaca gcgcgcccag tacgaacgtc ggctcggaga
     8221 gcggcacgcg cagcacggcc ggcagcccgg ccaccgcatg cgagccggcc agctccagca
     8281 accggttgtc gaggtaggtg tagagcactc cgccgagcac cgcgccccac ctgctgccgg
     8341 cgccgccgag caccaccatc accagcagcc cgagcgtcag ctcactggtg gtgagcgagg
     8401 gggtcgcccc gcccaccacc aacaggtggg ccacgccgcc gagggtcccg agcagcccgc
     8461 ccaccagcac ggcgagcagc tgcgcccggt acaccgacag gcccagcacc gccacccgct
     8521 cggggttctc ccgcgcagcc gcccagatgt gtcccacgcg tgagcgcacc agccacgcga
     8581 ccaccgccca gcacagcgcc aggtacgcca gtgcgagcca gtaccggtac ggcgcgttca
     8641 ccacaccgac cagcaggttc ggcaggccct cgcggttcag cggcaggccc tcctcaccgc
     8701 ccgtgaggcc gccggggttg cgcagcacga gaatcgaccc ggcctgggcg aacgccagcg
     8761 tcaccatcgc gaaggcgatg cccgtgacgc gcagcgcgac cgcgccgacg agcagtggca
     8821 gcagcactcc cacgaccacc gcggccccga cggcagcggc cagcccgact ccggggcggg
     8881 ccagcgccag cgctgcggtg taggaccccg ccgcgacgaa gagcgcgtgt ccgaacgaca
     8941 gcagcccggt gcgcccgaag agcaggtcgt aggacagcgc gacgccgccg aacaccaggc
     9001 agagtgccag cagctgcatc gaccccgggc tgttcaccag cccgggcagc accccgggca
     9061 gcggcaccga ggcgtacgga agggccgcga gcacgacgag gacggccacc ggcgcgacgg
     9121 ccgagcgcca ccctgcgcgg cgccgcgggg cctcgcccgc cgtcgcccgc tcccgcacct
     9181 gctccacgag cctcgtcgcg gccgccggtg cactcgcacg atcactcacg ctgctgccct
     9241 ccccgacaga ccgcccggcc gcacgagcag caccagcgcg agcagcagca ccaccgacag
     9301 gtcgcccacc cccgacgccg tgtagtagtt ggcgagctgc tgcaccagtc cgacgacgac
     9361 ggcggccacc gccgaaccgg tcagcgaccc cagcccgccg atcacgacga cgatgaacgc
     9421 gaaaatcagc agcgacgttc cctggcctgg cgacacgctg cccaggtaca ccgacccgag
     9481 cacaccgccg agcgccgcga ccgccccgcc gagggcgaag acgagcgtga acgtccggcg
     9541 gacgtcgatg ccgagcgcct cgaccatcga ccggttctcc acccccgccc tgatgatcaa
     9601 cccgatgcgg gtgaaccgga gcaacgccaa gagcccgagc agcacggcga tggcggccac
     9661 cagcaggacg aaccggtcgt tcgggatcgc ggccccaccg atcgtcgtga cccccgccgc
     9721 gacctccgga cgcgggaacg gcagcgggtc ggcgccccag atgccgcgca ggagtgccac
     9781 accgaccagt cccagcccca ccgtgaccag cacctgctcg atgtggcggc ggtagagcgg
     9841 gcggatcagg agcagctcaa cgctggcacc gatcacggcc ccgaccgcga cgccgaacgc
     9901 ggtggccagt acgaaacccc acccgtcacc gccggcaccg ggcagcccgc ctgctgcggc
     9961 ccaccaggcc gcataggcgc ctgccgcgag gaaggcgccg tgcgcgaagt tcagcacgcc
    10021 catcagcccg tagatcaggc tcagcccgga ggccacgagg aaatacagcg ccccgagccc
    10081 gaggccggtg gccaacaaca gcacgaaagt actcatcgca cggcctcgct gcgctcggcc
    10141 ggcgcttcgc agggacgctg tatcaatgat tcgagctcgc tcatgacttc gaccccaccc
    10201 cgagcagctc ccgcgtacgg ccggcgtcgg cgagcagctc gtcggcgcgt cccgcgtgca
    10261 cgacccggcc tgcgtcgagt accacgacgt ccccggcgag gcggcgcacc accggcaggt
    10321 tctgctccac cagcagcacc ggcgtcacct gcgccacccg ttccagcgcg gccgccacct
    10381 cagtgacgag ccgcggggcg aggccctttg tcggctcgtc gatcagcagg agccggctcg
    10441 gtgggagcag cgcccgcgcg agtgccacca tctgctgctg gccgccggag agcgtcccgg
    10501 cccgctgccg gctgcgctcg gccagctcgg ggaacagctc gtgcaccagc gccgcccgtt
    10561 ccggctcggg atcggggcac gccagccgca ggttctccgc cacggtcagc gtcgcgaaca
    10621 cctcgcggtc ctcgggcacg tatccgacgc cctggcgcac cacccacggg gtccgtccac
    10681 ggccgatctc gtcccctgcc agccggatcg agccggtgcg cggcaccagc ccgacgacgg
    10741 cacgcaatgt ggtggtcttg ccgacgccgt tgcggccgag cagcgcggtc acgccggtcg
    10801 gcgcgacggc cagcgacact ccctgcagga cgtgcgaacc accgatgcgc acgtgcacgt
    10861 cggccagctc gagaaccggc tcggtgctga tggctctcac agctcctccc ccaggtacgc
    10921 ctgctgcacg gtggcgtccg cggtgacctc ggcaggtgag ccgaccgcga gcagcctgcc
    10981 gtggtgcagc accgccaccc ggtcggccag cccgagcacg acgtccatgt ggtgttcgac
    11041 catgcccacg ctcaccccgg cagcgcgcag cccgccgatg agctcgacga ggggcggcac
    11101 gtcctccacg gacacgccgg ccatcggctc gtcgagcagc agcaccgacg gccgccgcgc
    11161 cagcgcgatg gcgatttcga gcttgcgctt gtcgccgtgg gcgagcgcac ctgccgtcgc
    11221 agtggcgcgg tgggccatcc ggacctgctc gagcagcgcg tcggcctcgg ccagctcctc
    11281 gcgcccctgc gccccgcgga gcacggacag cgggcgcgcg cggcggctct gcaccgccaa
    11341 ccggacattc tcccgcgcgg agagcccgcc gaacaggctg gaggtctgaa acgtccggcc
    11401 gagcccgtgc cgggcgcggg cggcggccga cgcgcgggtc acgtcccgcc cgcccaactc
    11461 gaccgtgccg ctcgtaggac gccggacgcc ggagagcagg tgcaggagcg tcgacttgcc
    11521 cgcgccgttg gggccgatca cggcgagcag ctcaccgtca cggacatcca ggtgcacatc
    11581 cgcgaggatc actgcaccac cgacgaccca ccccagatcg cgcgctgcga ggaccggtgc
    11641 cgccgtggcg gtggcgctca ccgggctcac ttggcgggtg cgacgggcgg cgccaccgcg
    11701 tccggcggaa gcgtcgcgac caactgcggg gcgaagccgc tcgccgtctg cgtgagccgg
    11761 gcggtgaaca tcggctgcag gagcgcgtgg tcctgcgccc tgatcgtcga ctcccccttc
    11821 gggccggtga acgcccagcc ctccagtgcc ttgaccatcg cagcggtgtc gtcgccgccc
    11881 tcctgcaggg cgtgcaccac catctgggcg gccacgaacc cgtcgttggt gaagaggtcg
    11941 acctcggcgc cctgcttcgc gaggcccgcc tccagtgcct tgtaggcctc gttgtccgtg
    12001 gcgccgggga agaagtgcga caggaactgg atcttgtcgg cagcagcgcc gaagatcggg
    12061 taggtcgagc ggatgtccag ccccgtcacc acggtggacg cctcgaacgc gccctgctgc
    12121 tgcaacgact gccacatctg ggtggcgttg gcgcctgccc atgccacgaa cagcaggttc
    12181 ggtttggcgt ccacggcctg ccgggcgaac ggggtgaggt cggtagcgcc cgcgggcacc
    12241 agcaccgggg tgacccgcgc ccccttcgcc ccgagcacgg cggtcacggc ctgcgcgttg
    12301 gcctgtccga aggcgctgtc ctgcgcgaag acggtcacgg aacggcccgc cgggtcgccg
    12361 atgatcgagc ccgcggtgag cacgtcctgg taggtctggc gccccgagcg gaacgtgtac
    12421 ttgttcacgc cggtgatccc gtcggtcgcg gcgggtccgg acacgaacag caccttgttc
    12481 tcctcggcga gcggcgccac ctgcaacgcg acgccggatg ccgtcgaccc ggcgatcacc
    12541 ttgtagccct ggccgatgag gtcggttgcg gcggcgacag ccttcgccgg gtcgcccgcg
    12601 tcgtcggcct cggtgatctg gacctgacga ccgttgacct gccccgtgcc cttggtggcg
    12661 tagtccaggc cggcgcggaa tccggcggag tactgcttgc cgtagctcgc gagcggaccg
    12721 gtggtggaat gcaccaggcc cacccggaac ggcgcggtcg cgctgttgcc accggcatcc
    12781 gaaccgccgg agccgggagc gccgcagccg gcgagcagca cggccaggac agcgatgagc
    12841 aaggccgctg gggaacgacc ggaacgttgg agcatgactc ctcctcgagc acgcgcgggg
    12901 ctgcgaggac gctacgaagc acggggcccg tcgaccatgg gtgcgaacgc catagcggtt
    12961 gcctccgcaa tgtggccagc gcactccccg cagagactcg cccgttcagc cctgccagat
    13021 cgccgctcag cgctcagaag cacctatcgg acgtcacatc gatcacagac cgtattcccc
    13081 cactcgacgg agagtacaga tcgatcgaaa ctccgcggaa ggtcgtgcgt tgtgccgagt
    13141 gaccggggcc ggcgggcaat ggggagcctc cgccggcacc ggttgatccc ccccggcggt
    13201 cagaccagcc ggaagaccgg ataccccggc gccatggccc gcagttgctc atcggttgcc
    13261 gaggcatcga cgccgtcgaa gaacaccccg acctcgaact tccagcgccg caggtactct
    13321 cgcaggacag cgggcttgtc gtcgtcggcg agctccgtgg ccgtgaactc ctcgacccgg
    13381 cggccgacac gcagctctcc tccgccggcg acgcgcaggt tgcgcaccca ttgggtgtgt
    13441 ccgcgcgggg ccaccaggta gcgcacaccg tcgtaggcga gcaggttgac ggggaccgtg
    13501 cgccactcgc cgctcttccg accacgaacg gccagcagcc gggaacccca gatgctgagg
    13561 cccatgcggg tgagccaggc gacgatgtcg ttgaagacgt cggtgccacg ggtggcgcgg
    13621 acgtagcggg cactgctcat ggtgatctct cctccgggat ccgatgcgcg agagcagtgc
    13681 tctcgcgcat gagcagtgaa ccagtaccgg tgagcacagt caagagcggt gctcttgttt
    13741 gagtttgccg ctctcaatcc tcgacgagca gcagctcgaa ggcgtcgtcc gcgatccaga
    13801 cccggatccc gtcgccgtca tcggtgatgt agccgtcctc ctcctcgccg agctcgcgag
    13861 aagcacccag ggcgaggcta ctgagttcga tgcgacccgc gccgacgtcg cgtggccggt
    13921 actcggacgc gagccgcgcc gtgcggctct cggtggagag ccagctggca ttgtcctcgc
    13981 cgacgtgggc cacccaccgc tgttcgcgca cgggccgagt atggcgtcgt cagcggggcg
    14041 tcaccagctc ggcgagcggt tcgggccggc ggacggcccg ccacacctga acgcgccggc
    14101 cgatggcgaa cgccagcgcc gtcaagacgg ccatcacgag accggcgagc aggctgagca
    14161 cgggactccc accggtgatc ggcccgagca gcagaccgat cagcagcatg tacaggctcc
    14221 aggccccgga gctgaccagc gaccagaaca ggaaccggtg cagccccagc ccgtagcggc
    14281 cggcggctgc cgtgctcacc aaccgcccgc cgggcacgaa ccgggctccc accaatgcga
    14341 tcccgggtcg cggcaggagg tgccggcgca cccagctcga caacccgcac tcgacgtcga
    14401 tcgtcttggc cagcacccgg tgcgaggagc gcccgaggcc gaacaccagg agatcgccgg
    14461 cggccgagcc gaccagcgcg gcgacgaaca ggccgaccac acccgtcacg tcgtggctcc
    14521 cgaacgctac tgcggcagcc gtcatcagca gtgtctcgct gggcaacacc gggaagggtc
    14581 cgtcgagcgc gatcaggacc acgagcacgg gtagcagcca cgggctgtac aggaccgcgt
    14641 cacacacggc caggacttcc ggcacgagag tcctctcggt tgcggtcgcc gcaggcgcga
    14701 ggcccacacg ggagagcccc cagcgtgtca gatggggaga ctgcggccag gggtggcacc
    14761 tggatagccc ggaagggggg tggcctcacc ctcgctgtga cgacactgct ccggatgcaa
    14821 ccaccccgtc gctcggcggc tcggctgcgc ctccgccctt acagtcgtgg cagcgcgctc
    14881 acgagggggt tcctgtgaat cgtcgtcgca ccgtgctcca cgccgtcgcg gcgctggtgg
    14941 ctgcagcagt actgggcgtg gccggaccag cgtcggctgc cgcggccgga ccggccgtac
    15001 caggacaggg gtcatcgggc gagcccatct cccccggcat cctcatgctc agccccgtca
    15061 gcgagagcgc cgccaacgcc tgcacggctt cgttcgtgtt caagagcggc ggcacgacct
    15121 acctcggata cgccgcccac tgcgccggaa ccagcctgac gggcctgagc ggttgccggg
    15181 agaagacgct gccactcggc agtaccgtgc tgatcgaacg cgggaacggc gccagcagca
    15241 ccggccggct cgcgtacaac tcgtgggcga cgatgcagcg gcggggcgag accgatcagt
    15301 cgctgtgcat gttcaacgac ttcgctctcg tcgcgctcga cccttcggac gccggagaag
    15361 tcgacccgag cgtgccgata ctgggcggcc cgaccgccct ggacacggac ggcttgcgga
    15421 gcggggaacc ggtcttcagc taccagccga acaacggcgg caccgtggtc aaggagggca
    15481 aaaccgtcag cgatagcggc ggcgggcggt cgcaccgcgt gaacaccacc ccaccaggtc
    15541 gccccggcga ctccggcagc ggctacctcg acggcaacgg catggcgttc ggcgtgctca
    15601 gcacgcagtt cctcgacggc accggcacca acgggctcac ggacctcgcg atggcgctga
    15661 cctacgccaa ccagtacggc ggcatcggcc gggtcgcgct cgtacccggg acggaaccgt
    15721 tcgctccacc tggatgaccc tcgatcgtcg actccacaag atgcagacgg tgatcggcgc
    15781 cgggccgcat gcgctgccct cctcctcaac ggaactggtc gacgttcgcc gcggcccact
    15841 cggcgaacgt cgtgcccggc cgcccggtga tctcggcgac cgtcggaacg gggcgctgcg
    15901 ggtgctgagc caacacggcc atcccttcga cgtaccactg ccccttcgac gtaccactgc
    15961 gcatactcac ccatggccgg cgtcagctgc tcgaccgcct gctcgtgggt cagctcgacg
    16021 tacgggatct cacgcccgag tccccgcccg atctgccgga ccatctcggc ccgggtgatc
    16081 gtctcgggac cggtgagttc ataggcggca ccggcgaggt gcgtccagtc gcgacctcga
    16141 cccccgacgg cagcgctgcc ttcaccggat tgttcgtcag cgccctgact cccaccagac
    16201 cggctaggcc tcgaacgtcg gctccggctc caccgccacc tccagtaaca gcggctccga
    16261 gcgcccggca agcgtgggga cgaccgcgtc gagggccgcg acgagcccgt cgtagctgtc
    16321 cacacgctcg gccgggcagc cgagggcctg cgccaaccgg tgcacgctga cctcctcgaa
    16381 cgccggccac ggcgccttcc cgctcccgtg ctgctcggcc agcctgtcca tgatggcgta
    16441 gcggccgttg gcgaggacga cgaacagcac gccgcagccg tagtgggccg cgctccacag
    16501 tgactggatc gcatagagcg acgacccatc cccgaccacg gcgaccaccg gatcgtccgg
    16561 tcgggccatc cgcaccccga tcgcagcggg catcgcgaac cccagcccgc ccatcgccgc
    16621 gctgaggaaa cccatcgggc gccgcgccgg aaccagccgg tgcaggtcgg gacgggtcga
    16681 cggcgtctcc tcgacgacga cggtctcgac cggtatccgc tcggcgagtg ccaccatcac
    16741 atgggcggcg cgcagcggct cccccgcccg cggtcgacgc ggcggcggga tgggatcggg
    16801 cggcgaaaca ggctcgtcgt cccgcacggg cacgcgcgcg gcgagctgcc ggcagacggg
    16861 cgcaggccgc gccaccacgg cgagatcgac agggctgtga tgggcgtctt cgacatcgtc
    16921 ggtgaccagt gcgaccttcg tcccgtcctc gaccagcggg cctggctcga acgggtactg
    16981 ccggaacacc ggagccccga cggcgagcac gacgtcgtgg ccggccagcg cctgccgcag
    17041 gcgggcccgg ccagggggaa ggtgccccca gaactgcggg tggtcctgcg ggaatccggc
    17101 gcgcgcggcg aacgattcct gccacaccgg gcagcgcaga cgctccgcga gctcgaccag
    17161 ggcagcccac gtctcggcgt cgtcggcgtc ggcacccacg acgagtgccg gtgactcgga
    17221 gcggctgatc atgtcggcga gcgcctcgac cgccgccggg tcgacggccg acgcgcggta
    17281 cagcaccgcg ggtgcgccca gcggggtgtc gtcgacagga gctgcccagt cgtcgctcgg
    17341 cacgatcacc actgccggac cgcggccgtg ccgtgcctcg tgccaggcac gggccaccgc
    17401 gccgggcacg tcctgcgggc gggggggtgt cgtgacccag accgggtacc ggccggccag
    17461 cccctcgagc tcaccggcga ggaacggctg caacgccaga tggcggcggt cctgctgacc
    17521 gacgagcacc acgagcggca cgcggttgac ccgggccgta gccagcgcgc cgacggcgtt
    17581 gccgaaacct gcggtggtgt gcaggacgac gaaagcgggc tggtccagtg ccgtcgccca
    17641 tcccgtcgcg atcccgacga ccgagccctc gtggagcgcg agcacgaagt cgacgtcgtc
    17701 gggcaggtcg gtcaggaacg ccacctcggt ggacccgggg ttggcgaaga tcgtggtcag
    17761 accgtggcga cgcaggacgt cgaacgtcgc gtcgcggacg gtgcgggcag tctcactcat
    17821 cctgcggttg tcctctcgac ggcggcgggt tcagcggtgc gggccaccag cgcgacggag
    17881 accgcaccga tcgcggccac cgtggcgaag atgtagaagc cccacgggta ggcgatcccc
    17941 gcgctgagca gcgcgccgcc gatgagcggc ccggagatcg cgccgagcct gccgaccccg
    18001 ctggcggcac cgagcgccgt accccgggcc gacaccgggt acaactggct cacgtaggcg
    18061 tagaccagca cctgagcgct gaacacgaac accccggcca tcagcacgct gacgtacacc
    18121 ccgatgccag ggagccggac gctcaacagc gccaggaaga gtgcggcggc cgtgaaccaa
    18181 gcgatcgtgg accgcctggt gccgaaccgg ttcgccacct gcccggcgac aagcagcccg
    18241 agcaccgcgc cgaggttgag cacgagcagc agtgccagcg cagcaccgag ggcgtacccg
    18301 gccgagcgca tgatctccgg cagccacgtg ttcaggccgt agaccaacag caaacccatg
    18361 aacgaggtca cccagaacgc gagcgtggcg cgcaggtacc cgccccggaa aaggatggtg
    18421 acggggttgc gctccggtcc ggtgctgccc ggcgttgctc cgagagcccg ctcgcgcagg
    18481 taggacgtcg actcgggcag gtaccggagc agcaacggca cgagcaccag cgcgggcagg
    18541 gccccgagca cgaacatcgg ccgccagccg aaggccggga tgaccacgat cccgagcagc
    18601 gcggtgacga ccgctccgac gtggtagccg gtcatcagcg tggtggtcgc gctgccactg
    18661 cggccctcgc gggcgtactc gttgacgagc gcgagcgcga ccggcagcac cccgccgaga
    18721 ccgaggcctg cgaggaagcg cagcaacccg aagatccacg ggttcggggc gaacgcgcac
    18781 aacagcgtga ggagcgagaa gccgacgacg gtcagcagca tcgtccggcg acgcccgatg
    18841 atgtccgtga cgggcccgat ggcgagcgcc ccgatcatca ccccgagtag gccgaccact
    18901 gagatcagcg atgccgtgcc cggcgtcagt ccccagtctg ggtaccgcag cagcggcggc
    18961 agcacgacgc cgagtacgac caggtcgaag ccctcgagcg caacggccga ccagcacagc
    19021 ggggccaccc agccggcgta ctgctcgcgg gggttctgcg ggttcaccgg tgaatccgta
    19081 gacatttcat cacctttctc ctggttccgc gaagtatcga ggtcacgtct cacgccgtca
    19141 gcaccctctt ccactcagcg gatgcttgcc ggccgatgag ttccgttggg caggacggtc
    19201 ctaccgccga ccatccacgc atgcctggcc ggaaggaaga tccgatgacc tccaccacca
    19261 cctccaccaa ccgcgtcacc aaccgcgtcc tgtggggtat ccaaatcctg gtcggaatct
    19321 tcttcatcgt cgcctcagcc gccccgaagt tcttcggtga ggcctacgcg gtggagatct
    19381 tcacgcagat cggggccggt cagtggttcc ggtacctcgt agcggtgctg gaactggccg
    19441 gcggaatcgg gctcatgacc acacggctcg cagctccggc cgcgctcgga ctcatggggc
    19501 tcatgatcgg ggccgcgttc acacaggcgg tggtgctcga cgcacccgcc atggtcctga
    19561 cgcccgcgat cctcttcgtg gtgctcgccg cgatcgcctg gggccgccgg gcgcagatca
    19621 ccgcgcagct cccctcccag gcgggctgac cggcctcaga tcgtccgcag gcttccgccg
    19681 tcgatgacgt gctccgcgcc cgtgatcgcc gacgcccgat cggacacgag gaaggcgatc
    19741 agctcggcca cctcctccgg gcgggccggg cggccgagcg ggatcccgcc gatcgagtcc
    19801 atgatccgcc ggcgggaggt gttctcgtcc acgccgtccg cggccgcgag cgcgctgacc
    19861 atcccgcgcg ctcccgccgt ctcgacgaac ccgggcgcaa ccgtattgac ccgcaccccc
    19921 ttcggggcca tctgggtggc gaggttcttg ctgtagctcg tcaacgcggc cttggccgcc
    19981 gcatagggga cggtcgtggg tagcggtacc cggcgctgga tcgaggtgac atggacgatc
    20041 gctccccggc cctggtcgat catcgacggg agcagggcgc ggtccaggcg caccgaggcg
    20101 aacaggttga cggcgaaggc gtcctgccag tcgtcgtcgg acagcgcgag aacaccgccg
    20161 ggctcctggc gcgatccgcc gaccacgtgg accacgatgt ccacgccacc cagccggttc
    20221 agggccgcct cggcgacccg ggccaccccc tccgccgtgc cgacgtcgga cgccacgaac
    20281 agctcggggt ggaccacgtc gtcaggggtc gatcgcgcgg tggtgagcac gcgcgcgccg
    20341 gcgtcggtga gccgggcggt gaccgcggcc ccgataccgc cgcgggtgcc accggtgacc
    20401 acggcccggc gcccgtcgag ttccctcatc ccgccgctca cggccagatc gtgagggccg
    20461 cgatccggtc cccggagtcg gtgaaatggt ggtgcaggtc caccgggctc cccgggaagt
    20521 tcccggcgac ccggacgagc atcaccgtgg ccgcgccggt gcggcgcgtc tcgagcactg
    20581 tgcgctcgag ctggtaggtc gtggagatcg cggcggccca gtcacggatc gccgcgatgc
    20641 cgacgtggtc cttgccctcg tcgtggacga tcgcgtcgtc ggtgaagctc tcgagtaccg
    20701 cctcgacgtg gtcgtcgtcc ttcgcctcca ggtatgcggc gacgggcgcg ggaaggttcg
    20761 tggacataag tgtctcctga atcgtggtgc gaacgtgagt gcgtgggcgg ccgaggcgcc
    20821 gcggcggtca gcgtcctgtg gtggtgaggt cctggtcgcc gatgacgcgc agcagcccga
    20881 gcttctcggc ggtctcgctg cccggtggcg gggtgaagac gagcagcttc tggtcctcgg
    20941 aaggggtcag cagggtctcg ctgtcgagct cgatcagccc gatccgggga tgcaggacgc
    21001 gcattcggct gcgcctgcgg acggccacct cgtgccggtc ccagagctcc gcgaactccg
    21061 ccgtggtcgc ccgcagccgg cggaccagcc cggctgcggc ggcatcgttg ccgcgacggg
    21121 cgacagcggc gcgcaggtcg gcgacgtgga cccggctgag gtactcgtgc tcctcgggcg
    21181 gatgcgctgc gcgtacaccg gggtcggtga accaccgcac cacgacgttg cgggactcct
    21241 cacgctccga gcacaccgct ccgaacaggt acctggccat ctcgttctgg gcgagcaggt
    21301 cgccgaggtc gcccaggatc tgcgcaggtg tggccggcag cgcgtcgagc aggtgcatca
    21361 gcccgggccg gacgtggtcg cccgcgagcc gccccgccgg tgcagggtga cctgccagga
    21421 cgtagaggtg gtcgcgctcg tcggcggtga gccgcagcgc gcgggccagc gccgcgagca
    21481 cctgcgtgga gggctgcgag ctgcgctgct gctccaggcg catgcagtag tcggcggaca
    21541 cccccgccag ctgcgccacc tcctcccgac gcaggccggg ggtgcgccgc cgcatcccgg
    21601 cagcgagccc gacgtccgcc gggcgcagct gctcccggcg gcggcgcagg aagtcggcga
    21661 gctcgcggcg gtccatccac ccgagcatca cccgtttccc cgccctgtgc cacggagagc
    21721 ggctcctagc ctgcgggcat aaccccgtgc gtgcatgcgt tgacgagctc gtactacttg
    21781 cagttgcaac aactggaggc gagacaatgc agctcggcat cttctcggtc ggcgacgtga
    21841 cgcccgaccc gacgaacggc cgcacgccga ccgaggcgga gcggatcaag gcgatggtcg
    21901 cgatcgcgct gaaggccgag gaggtcggcc tggacgtgtt cgcgaccggc gagcaccaca
    21961 acccgccctt cgtgccgtcg tcgccgacta ccatgctcgg tttcatcgcc gcgcgtaccg
    22021 aacgcctgca gctctccacc gcgacgacgc tgatcaccac gaacgacccg gtgaagatcg
    22081 ccgaggacta cgcgatgctg cagcacctcg cggacggccg ggtggacctc atgatgggcc
    22141 gtggcaacac cgggcccgtc tacccgtggt tcgggcagga catccgcaac gggatcgcgc
    22201 tggccgtcga gaactacgcg ctgctgcgca ggctgtggcg cgaggacgtc gtcgactggc
    22261 agggcaagtt gcgcacgccc ctgcagagct tcacctcgac gccgcgaccc ctcgacggcg
    22321 tgcccccgtt cgtctggcac gcctcgatcc gcagcccgga gatcgcggag caggcggcct
    22381 tctacggcga cggcttcttc cacaacaaca tcttctggaa caaggagcac gtgcagcgga
    22441 tggtggagct gtaccgcagg cgcttcgagc actacggcca cggcgctgcc gaccaggcga
    22501 tcgtcgggct gggcggccag gtgttcatac ggaagaactc ccaggacgcc gtccgcgagt
    22561 tccgcccgta cttcgacaac gcgcccgtgt acggccacgg cccctcgctc gaggagttca
    22621 cggacctgac agcgctcacg gtgggcagcc cgcagcaggt gatcgagaag acgctgtcct
    22681 tccgcgagat ggccg
//
LOCUS       DASRSC010000031         2620 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4103576,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000031 DASRSC010000000
VERSION     DASRSC010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4103576"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VFE39_01485"
     CDS             complement(<1..637)
                     /locus_tag="VFE39_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HET6257300.1"
                     /translation="MDRVFSAADVTAAATRLTGVARQTPLHHNDRLTAATGVQVWLKR
                     EDLQVGRSYKLRGAYNLLAQLDDAERAAGAVCASAGNHGQGVAYACRQLGIRGRVHVP
                     STTPRQKRERIAALGGDAVELVVGGDTYDEAAAAAADYAVRTGATVVPAFDDLRTIAG
                     QGTVGVEIVTQLGRAPDVVVVPVGGGGLLAGVGGWLAAHHPDVRIVGVEPAG"
     gene            complement(686..1687)
                     /locus_tag="VFE39_01490"
     CDS             complement(686..1687)
                     /locus_tag="VFE39_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6257301.1"
                     /translation="MEPLRVGVLGAARIAAAAIVEPAQLTGTRLVAVAARDRARAEAF
                     ATEHDVERVLGSYADVLADPEVEAIYNPLANGLHGPWNLAAVAAGKHVLTEKPSASDA
                     TEAREVRDAAERAGVVVMEGFHYLYHPVARRLLDVLASGEIGELRRVEIDMVIPPPPP
                     GDLRWSFPLAGGALMDVGCYGLHMHRALTPSAGGAPRVVAARAGERAGSPGVDEWLDA
                     DLEFPGGATGTVRCSMAAESTRMTCRIVGSRGEVSAPQFVLPHRDDRVLVTAGGSDRV
                     EELGRRPSYAYQLEAFALHVRGGEPVPTGADDAVATAELIDDCYRAAGLPLRPRTHL"
     gene            complement(1739..2086)
                     /locus_tag="VFE39_01495"
     CDS             complement(1739..2086)
                     /locus_tag="VFE39_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HET6257302.1"
                     /translation="MSASNGIEPAVAPSGLGCVECDEVGGWWVHLRRCARCGHVGCCD
                     TSPAQHATAHFRGTGHPYVRSFEPGESWYWDYRSSEMLDGPELADPQHHPLDQPVPGP
                     AGRVPSDWRRHIH"
     gene            2206..>2620
                     /locus_tag="VFE39_01500"
     CDS             2206..>2620
                     /locus_tag="VFE39_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6257303.1"
                     /translation="MAAKPFASSSDLAAKEQTLEVLADGVYALTAEGDPNIGAVEGED
                     FVVCFEALATPVAAQKWLARLREYTDKPVRYLVLSHYHAVRVLGASAFDDEVIVAHEN
                     TRALVAERGKEDWESEFARMPRLADGAESVAGLTWP"
BASE COUNT          353 a          952 c          970 g          345 t
ORIGIN      
        1 cgccggcggg ctccacgccc acgatccgga cgtccgggtg gtgtgccgcc agccagccgc
       61 ccaccccggc gagcagcccg cccccgccga ccggcaccac gacgacgtcc ggcgcgcggc
      121 ccagctgcgt cacgatctcg acgccgacag tgccctgccc ggcgatggtg cgcagatcgt
      181 cgaaggcggg gacgacggtg gcgccggtgc ggacggcgta gtcggccgcg gccgcggccg
      241 cctcgtcgta ggtgtcaccg cccaccacga gctcgacggc gtcgccgccc agcgccgcga
      301 tccgttcgcg cttctgccgc ggcgtggtgc tcgggacatg cacgcgacca cgaatgccga
      361 gctgccggca ggcgtacgca accccctggc catggttacc cgcgctggcg cagactgcgc
      421 ccgcggcgcg ctcggcgtcg tcgagctgag ccaggaggtt gtacgcaccg cgcagcttgt
      481 acgagcgccc gacctgcaga tcctcacgct tgagccacac ctggacgccg gtggcggcgg
      541 tcaggcggtc gttgtgatgc agcggggtct ggcgagcgac gccggtgagc cgggtggcag
      601 cggcggtgac gtcagcggca gagaacaccc tgtccatcct ccaccttccg cggccctgcc
      661 cgccgcgagg gtcgcggggc ggccgtcaca ggtgggtgcg tggacgcagc ggtagcccgg
      721 ccgcgcggta acagtcatcg atgagttcgg ccgtcgccac ggcgtcgtcg gcgccggtgg
      781 gcaccggctc gcccccgcgg acgtgcaggg cgaaggcctc cagctggtag gcgtaggacg
      841 gacggcggcc cagctcctcg acgcgatcgg agccgcccgc cgtcaccagc acgcggtcgt
      901 cgcgatgcgg cagcacgaac tggggtgcac tgacctcccc gcggctgccg acgatgcggc
      961 aggtcatccg cgtgctctcg gccgccatcg agcaccgcac cgtgcccgtc gctccgccgg
     1021 ggaactccag atccgcgtcg agccactcgt ccacgccagg actgccggcc cgttcgccgg
     1081 cccgggcggc cacgacccgg ggcgcaccgc ccgccgacgg cgtcaacgcg cggtgcatgt
     1141 gcaggccgta gcacccgaca tccatcagag cgccgcccgc cagcgggaac gaccagcgca
     1201 ggtcaccggg agggggcggg gggatcacca tgtcgatctc gacgcggcgc agctcgccga
     1261 tctccccgga cgcgagtacg tcgagcagcc ggcgcgccac cgggtggtag aggtagtgaa
     1321 agccttccat gacgacgacg ccggcccgtt cggccgcgtc gcgtacctcg cgggcctcgg
     1381 tcgcgtcgct ggccgagggc ttctcggtga gtacgtgctt gcccgcagcc accgcggcga
     1441 ggttccacgg accgtgcagt ccgttcgcga gcgggttgta gatcgcctcc acctcggggt
     1501 cggcgagcac gtcggcgtag gagcccagta cccgctcgac gtcgtgctcg gtggcgaagg
     1561 cctcggccct ggcccggtca cgggcggcaa ccgccacgag ccgggtgccg gtgagctggg
     1621 caggttcgac gatcgcggcc gcggcgatcc gtgcggcgcc gaggacgcct actcgaaggg
     1681 gttccacggc ttgatcctgc ctgcgggccg tccgcgggcc gcgctccgcc cccggcgatc
     1741 agtggatgtg ccggcgccag tcgctcggca cccggcctgc cggcccgggc acgggctggt
     1801 cgagcgggtg gtgctggggg tcggcgagct cgggaccgtc gagcatctct gagctgcggt
     1861 agtcccagta ccaggactcg ccgggctcga aactgcggac gtacgggtgc ccggtgccgc
     1921 ggaagtgggc cgtggcgtgc tgggccggtg aggtgtcaca gcagccgacg tggccacacc
     1981 gggcgcaccg gcgcaggtgc acccaccagc cgccgacctc gtcgcactcg acgcagccca
     2041 ggccgctcgg tgcgacggcg ggctcgatcc cgttcgaagc ggacatgcgc atctcctcct
     2101 gggtgctcga ctgggcccat catggacgcg cgaagggttc ccgcgcggga cggtcccggg
     2161 taccgtcccg gttggtcaaa cctttgatcg aagacggagg cgttgatggc agcgaagccg
     2221 ttcgcatcgt ccagcgacct ggccgcgaag gagcagacgc tcgaggtgct cgccgacggc
     2281 gtctacgcgc tgacggcgga gggcgatccc aacatcggcg ccgtcgaggg cgaggacttc
     2341 gtcgtctgct tcgaggcgct cgccactccc gtcgcggcgc agaagtggct ggcccggctg
     2401 cgggagtaca ccgacaagcc cgtgcgctac ctcgtgctca gccactacca cgccgtccgg
     2461 gtgctcggcg cctccgcgtt cgacgacgag gtgatcgtgg cgcacgagaa caccagggcg
     2521 ctggtcgcgg agcggggcaa ggaggactgg gagagcgagt tcgcccggat gccgcgcctg
     2581 gccgacggcg ccgagtcggt cgccggcctc acctggccga
//
LOCUS       DASRSC010000032         8292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_337194,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000032 DASRSC010000000
VERSION     DASRSC010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8292
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337194"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1777
                     /locus_tag="VFE39_01505"
     CDS             <1..1777
                     /locus_tag="VFE39_01505"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6257304.1"
                     /translation="PRFSDLPLRAELLAIVDELGYTHPSPIQAESIASLVEGRDLLGQ
                     AATGTGKTAAFALPMLERLAEQRPERARGDAPFGLILAPTRELALQVSEAVTRYGSRL
                     GARVLTVYGGAPVGPQWKALQNGVDIVVATPGRAIDLMNRGALHLDDLETVVLDEADE
                     MLDMGFVEDIETLLDATPDTRQAVLFSATMPRRIETLAQKYLREPVTVRIRREAVPAG
                     EAPKVRQTAYLVPRTHTTAALGRVLEAERPAAAIVFCRTRLDVDAVTETLTGRGLRAE
                     ALHGGMDQEHRTRVVERLRSGRTELLVATDVAARGLDIDLLTHVVNHDVPQSAEAYVH
                     RIGRVGRAGREGVAITLVPPSKVHALRSVERLTGQPIAFAPVPTAADLRAARLERTSA
                     ALRERLVADLVGGSDTVDTDTVDIHTVDADVASTPDRDGVRAMLAGLAAEFDAVDVAA
                     AAVRLMQETAGSPEDGEDIPVVTAPRSSGTREPRDTRGRGGAAGIRPPKAGTTRLFVN
                     AGRASGVRPQDIVGALANESNLSGRDIGAIQIHERHALVEIPEHVADDVLRSLRGSTT
                     LKGRRANVRRDRGFASAGAGRARRD"
     gene            complement(1853..2173)
                     /locus_tag="VFE39_01510"
     CDS             complement(1853..2173)
                     /locus_tag="VFE39_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257305.1"
                     /translation="MEPEERFAALVDELAAEPGVIRPGADGGRGFGSAALKVNGSIFA
                     MLTRERLVVKLPAARVAQLVAAGAGDPFDAGAGRPMRQWLTVGAEQDWQALAREALTF
                     AGRR"
     gene            2288..3643
                     /locus_tag="VFE39_01515"
     CDS             2288..3643
                     /locus_tag="VFE39_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6257306.1"
                     /translation="MAEALAIKDPVVEGAVRRARWRLLPFLGLCYLLNYVDRVNVGFA
                     SLTMNSDLGLSAAAYGLGAGLFFIGYFFFEVPSNVILHRVGARLWIARIMITWGVVAS
                     ATAFVQGEISFYIVRFLLGIAEAGFFPGIILYLTYWFPRAQRAKVVALFFLAVPLSSV
                     IGAPISSLLVQNGAGVLGFDAGWRFMFFVEGIPAMLVGVLVLVLLPDRPSRARWLSGR
                     EQHALEQRIADEDAAQVPQETGLWAGLRDGRVVALSIVYFGVVFGLYVLAFFLPQVVS
                     GLQEQFDVRFSLVEIGLITAVPYAVASVAMVLNARHSDRTGERRLHVAVPAFVGAAGV
                     AVALYLDSPYLVIAAMTVCAVGVFSAIPVIWQLPSAFLTGVGAAAGIALINSFGNLSG
                     FAGPYLTGWLQDLTGSFRAGMFVVAGFMVLAGVIVLFVGRDSRPAEDTGDATTAQAQR
                     G"
     gene            3691..5373
                     /gene="ilvD"
                     /locus_tag="VFE39_01520"
     CDS             3691..5373
                     /gene="ilvD"
                     /locus_tag="VFE39_01520"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET6257307.1"
                     /translation="MTQTQDLQPRSRDVTQGLERAAARGMLRAVGMRDEDFVKPQIGI
                     ASSWNEITPCNLSLQRLGIAAKEGVHRGGGFPMEFGTISVSDGISMGHIGMHWSLVSR
                     EIIADSVETVVQAERLDGTVLLAGCDKSLPGMLMAAARLDLAAVFVYAGSILPGYVDG
                     REVNIADAFEAVGACARGLISREEVDAIERAICPGEGACGGMYTANTMASAAEALGMA
                     LPGSASPPAVDRRRDGIARASGEAVVGMIEQGITARQILTREAFENAIAVVMAFGGST
                     NAVLHLLAIAWEAGVPLELDDFNRIGDRVPHLADVKPFGSHVMSTVDQVGGVPVVMKA
                     LLGAGLLHGDVLTVTGKTVAENLAAIDPPDLDGSILRSLDNPIHPTGGITILRGSLAP
                     EGAVVKSAGFDSSRFEGTARVFDGEQGAMTAVEDGTLQPGDVVVIRYEGPRGGPGMRE
                     MLAVTGAIKGAGLGKEVLLLTDGRFSGATTGLCVGHVAPEATDGGPIALVCDGDTVVL
                     DMERRTLDLLVDDAELARRRAEWSAPAPTLRTGVLGKYAKLVGSAAQGAVCG"
     gene            complement(5451..6158)
                     /locus_tag="VFE39_01525"
     CDS             complement(5451..6158)
                     /locus_tag="VFE39_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005162903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HET6257308.1"
                     /translation="MKVEIWSDVVCPWCAIGKRRFEMALAQFAHRDEVEVTWRSFELD
                     PGAPRERAGDLREHLATKYGVSQEQADGMHRRMTETAAADGWEFHFERARGGNTFDAH
                     RLIHLAGERGIQDAVKERLFTAYLTDGERIGDPETLVRIGTEAGLDADEARAVLDSDR
                     FADEVRADERQAQAFGITGVPFFVIDRTYGVSGAQPAEALLEVLDKAWAETHPLQVLT
                     PAGGAAGDACVDGSCSV"
     gene            complement(6166..7539)
                     /locus_tag="VFE39_01530"
     CDS             complement(6166..7539)
                     /locus_tag="VFE39_01530"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HET6257309.1"
                     /translation="MTVHRSVLDAVGGTPLIQLNRITAGIAAAVHVKVEFANPGGSVK
                     DRAALAMVREAERLGHLQPGGVIVEGSSGNTGVGLAMVAAQHGHRAIVVVPDTIAREK
                     VALLRAYGAQVVQTVGKLPREHPEHVNNLARRIAAETPGGWHANQYDNPANPRVHELT
                     TGPEIWEQTDGRVTHLVAGVGTGGTISGTGRYLKQHGVTVVAADPLTSVYSGGDGSPY
                     FVESIGHYLHPSTVDDVWPQAFDQGVIDRFERIPDRESLAVARRLAREEGLLVGGSAG
                     TAVAAALRVAAELGPEHVVVAILPDSGRSYLSKYFDDDWMTQLGFLDAPHGPFVRDVV
                     QEHGLAVAHAGHSLAAVLDIHSDGLVAVVTPRDSDTPTRSAPEVIGVVEPAALRALVA
                     TDPARSDDDVTAHAAPAPVAVGAGENLAAARARLAGDAPVLVLVDGRAVTVVARSALE
                     QVPVTSR"
     gene            7637..8215
                     /locus_tag="VFE39_01535"
     CDS             7637..8215
                     /locus_tag="VFE39_01535"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6257310.1"
                     /translation="MPDLPRDWNQWREQIDLDRYDERWRRMAAAGGNPHGEADLIESY
                     QPGSVLDGGCGTGRVGIELARRGIAVLGVDADAAMIAAAQAKAPDLEWVAVDLAELAR
                     PERFDLVALAGNVVPYVAPDRRRDAVAACAGHLRPGGRLVAGFQLQDGWPTLGDYDGW
                     CRAAALNLEDRFATWDREPYAGGAYAVSVHRL"
BASE COUNT         1084 a         3028 c         2967 g         1213 t
ORIGIN      
        1 cccccgattc tccgatctcc ccctccgcgc cgagctgctg gccatcgtcg acgagctcgg
       61 ctacacccac cccagtccga tccaggccga gtccatcgcc tccctcgtcg aggggcgaga
      121 cctcctgggc caggcggcca ccggcaccgg caagacggcg gcgttcgcgc tgcccatgct
      181 ggagcgcctg gccgagcagc gccccgagcg ggcgcgcggc gacgcgccgt tcgggctgat
      241 cctcgcgccc acccgcgagc tggcactgca ggtctccgag gccgtcaccc gctacgggtc
      301 gcggctgggc gcccgcgtcc tcacggtgta cggcggcgca cccgtcggcc cccagtggaa
      361 ggcgctgcag aacggcgtcg acatcgtggt ggccacccct ggccgcgcca tcgacctgat
      421 gaaccgcggt gccctgcacc tggacgacct cgagaccgtg gtgctcgacg aggccgacga
      481 gatgctcgac atgggtttcg tggaggacat cgagaccctg ctcgacgcca cccccgacac
      541 gcggcaggcc gtgctgttct cggccaccat gccgcggcgc atcgagaccc tcgcgcagaa
      601 gtacctccgc gagccggtca ccgtccggat ccgccgcgag gccgtgccgg ccggcgaggc
      661 acccaaggtt cgccagaccg cgtacctcgt cccgcgcaca cacacgaccg cggcactggg
      721 tcgtgtgctg gaggccgagc ggcccgccgc cgccatcgtc ttctgccgta cccggctcga
      781 tgtcgacgcc gtcaccgaga cgctcaccgg ccgtggcctg cgcgccgagg ccctgcacgg
      841 cggcatggac caggagcacc gcacgcgcgt ggtcgagcgg ctgcgcagcg ggcgcaccga
      901 gctgctggtc gccactgacg tggctgctcg cggcctcgac atcgacctgc tcacccacgt
      961 ggtcaaccac gacgtgccgc agtcggcgga ggcctacgtt caccgcatcg gccgcgtcgg
     1021 gcgggccgga cgtgagggcg tcgcgatcac actggtgccg ccgtcgaagg tgcacgcgct
     1081 gcgctccgtc gagcgcctca ccgggcagcc gatcgcgttc gccccggtgc ccaccgccgc
     1141 cgacctgcgg gccgcccggc tggagcggac ctccgcggcg ctgcgcgagc ggctggtcgc
     1201 cgacctcgtc ggcggctcgg acaccgttga caccgacacg gttgacatcc acacggttga
     1261 cgccgacgtc gccagcacgc ccgaccgcga cggggtccgc gcgatgctcg ccgggctcgc
     1321 cgccgagttc gacgccgtgg acgtcgccgc cgcggcggtc cgcctgatgc aggagaccgc
     1381 gggctcaccg gaggacggcg aggacatccc ggtcgtcacc gcgccgcgct ccagcggaac
     1441 ccgtgagccg cgcgacacgc gcggccgcgg cggcgcggcc ggcatccgcc cgccgaaggc
     1501 cggtaccacc cgcctgttcg tcaacgcggg ccgcgccagc ggcgtccgcc cccaggacat
     1561 cgtgggtgcg ctcgccaacg agtcgaacct gtccggccgc gacatcggcg cgatccagat
     1621 ccacgagcgg cacgcgctgg tggagatccc cgagcacgta gcggacgacg tgctgcgcag
     1681 cctgcgtggc agcaccacgc tgaagggccg gcgggccaac gtccgccgcg accgcggctt
     1741 cgcttcggcg ggtgccggcc gcgcccgccg cgactgaccg acggcaaccg cgtcgcggag
     1801 ccgtatcgct gcctggatgc cagtaccagg tgcggtacgg ctccgtggtg cgtcagcggc
     1861 ggcccgcgaa ggtcagtgct tccctggcga gggcctgcca gtcctgctcc gcccccaccg
     1921 tcagccactg gcgcatcggg cgtcccgcgc ccgcatcgaa cgggtcgccc gcgccggcgg
     1981 cgaccaactg tgcaacacgg gcggcgggca gcttcaccac cagccgctcg cgcgtgagca
     2041 tggcgaagat cgagccgttg accttcagcg cagcggagcc gaacccgcga ccgccgtccg
     2101 cgccgggccg gatgacgccg ggctcggcgg cgagctcgtc caccagcgca gcgaaccgct
     2161 cctcgggttc cacgccaccg atccaagcgc agctcccggc tctgccgcca tgcccacacg
     2221 gcgccaccgg ttgtcccgtg gccgccgcgc cggtaggagt tgacctccac gaactcgagg
     2281 gggagtcatg gcggaggcgc tggcaatcaa ggacccggtg gtcgaggggg ccgtgcgccg
     2341 agccaggtgg cggctgctgc cgtttctcgg cctctgctat ctcctgaact acgtcgaccg
     2401 cgtcaacgtc gggttcgcct cgctcacgat gaactccgac ctcgggctct ccgcggcggc
     2461 gtacggcctc ggcgccgggc tgttcttcat cggctatttc ttcttcgagg tgccgagcaa
     2521 cgtcatcctg caccgcgtcg gcgcccgcct gtggatcgcc cgcatcatga tcacctgggg
     2581 tgtcgtcgcg tcggccacgg cgttcgtgca gggcgagatc agcttctaca tcgtgcggtt
     2641 cctgctcggc atcgccgagg cgggcttctt ccctggcatc atcctgtacc tgacgtactg
     2701 gttcccgcgg gcgcagcggg cgaaggtcgt cgcgctgttc ttcctcgccg tcccgctgtc
     2761 gtcggtaatc ggcgcgccga tctcctcgct gctcgttcag aacggcgccg gggtgttggg
     2821 cttcgacgcc gggtggcggt tcatgttctt cgtggagggc ataccggcaa tgctcgtggg
     2881 cgtgctcgtt ctggtgctgc tcccggaccg gccgagccgg gcccgttggc tctcggggcg
     2941 cgagcagcat gccctggagc agcggatcgc ggacgaggac gccgcgcagg tgccccagga
     3001 gaccgggctg tgggcaggct tgcgggacgg gcgggtcgtc gcgctgtcga tcgtctactt
     3061 cggtgtcgtg ttcggtctgt acgtgctggc gttcttcctg ccgcaggtgg tgtcgggcct
     3121 gcaggagcag ttcgacgtcc ggttttccct cgtcgagatc gggctgatca ccgccgtgcc
     3181 ctacgccgtc gcgtcggtgg cgatggtgct caatgcccga cactccgacc gcaccggcga
     3241 gcgacggctg cacgtcgccg tgcccgcgtt cgtcggggcc gcgggcgtcg cagtggcgct
     3301 gtacctcgac tcgccctacc tcgtgatcgc ggcgatgacg gtgtgcgcgg tgggtgtctt
     3361 ctcggcgatc cccgtgatct ggcaactgcc gagcgcgttc ctcacggggg tcggtgcggc
     3421 ggccgggatc gcgctgatca actcgttcgg caacctcagc ggcttcgccg gcccgtatct
     3481 gaccggctgg ctgcaggacc tcaccggcag cttccgagcc ggaatgttcg tcgtggcggg
     3541 gttcatggtg ctggccgggg tgatcgtcct gttcgtcggg cgcgactcgc ggccggccga
     3601 ggacactggg gatgcgacga cagcacaggc acagcgagga tgacccgctc gcgagccacc
     3661 aggcccaccg cagctcggga ggaaccaccg atgacccaga cgcaggatct ccagccacgc
     3721 agccgcgatg tcacgcaggg gctcgagcgc gccgccgcgc gcgggatgct gcgggccgtt
     3781 ggcatgcgcg acgaggactt cgtcaaaccg cagatcggga tcgcgtcgag ctggaacgag
     3841 atcacgccct gcaacctctc gctgcaacgc ctcggtattg cggcgaagga gggcgtgcac
     3901 cggggcggcg gcttcccgat ggagttcggc accatctcgg tctccgacgg catctccatg
     3961 ggccacatcg gcatgcactg gtcgctggtc agccgggaga tcatcgccga ctccgtcgag
     4021 accgtggtgc aggccgagcg gctcgacggc accgtgctgc tggccggctg cgacaagagc
     4081 ctgcccggga tgctgatggc agccgcgcgg ctcgacctcg ccgcggtgtt cgtctacgca
     4141 ggctcgatcc tgcccggcta cgtggacggc cgcgaggtca acatcgccga cgcgttcgag
     4201 gccgtcgggg cgtgcgccag aggcctcatc tcccgggagg aggtcgacgc gatcgaacgc
     4261 gcgatctgcc cgggcgaggg cgcctgtggc gggatgtaca ccgcgaacac catggcttcg
     4321 gccgcggagg cgctcgggat ggccctgccc gggtcggcga gcccgcccgc tgtcgaccgg
     4381 cgccgcgacg gcatcgcccg cgcgtccggg gaggccgtcg tcgggatgat cgagcaggga
     4441 atcacggccc ggcagatcct caccagggag gcgttcgaga acgcgatcgc cgtcgtgatg
     4501 gccttcggcg gctcgacgaa cgccgtgttg cacctgctcg cgatcgcgtg ggaggcaggc
     4561 gtgccgctgg agctcgacga cttcaaccgg atcggcgacc gcgtgccgca cctggccgac
     4621 gtcaagccgt tcgggtcgca cgtcatgtcc acggtggacc aggtcggcgg cgtgccggtg
     4681 gtgatgaagg cgctgctggg cgccgggctg ctgcacggcg acgtactcac cgtcacaggc
     4741 aagacggtcg cggagaacct ggccgcgatc gacccgcctg acctcgacgg cagcatcctg
     4801 cggtcgctcg acaatccgat ccaccccacc ggcggcatca cgatcctgcg cggctcgctc
     4861 gcccccgagg gggccgtcgt gaagagcgcg ggcttcgact cgtcgcggtt cgagggcacc
     4921 gcgcgagtgt tcgacggcga gcagggcgcg atgacggccg tcgaggacgg caccctccag
     4981 cccggcgacg tcgtcgtgat ccgctacgag ggcccgcgcg gcggcccggg catgcgggag
     5041 atgctggccg tcacgggcgc gatcaagggc gcggggctgg ggaaggaggt cctgctgctg
     5101 accgacggcc ggttctccgg ggccaccacc gggctctgcg tcgggcacgt ggcgccggag
     5161 gccaccgacg gcggcccgat cgcgctggtc tgcgacggcg acaccgtcgt gctcgacatg
     5221 gagcgccgca cgctcgacct gctggtggac gacgcggagc tcgcccgccg ccgggccgag
     5281 tggtcggccc cggccccgac gctacggacc ggcgtgctgg ggaagtacgc caaactcgtc
     5341 ggctccgcgg cgcagggggc ggtctgcggc tgaccccgcc ccccaacccc cgcgagtcgc
     5401 ccgtttcccg cccgcgagtc gccgggtttc ggcccgcgag tcgcccgttc tcagacggag
     5461 caggagccgt cgacgcaggc atcgcccgcg gcgccccctg ctggcgtcag tacctgcagg
     5521 gggtgcgtct ccgcccaggc cttgtccagc acctcgagca gggcctcggc cggctgcgcg
     5581 ccggacaccc cgtacgtgcg gtcgatcacg aagaacggga cgccggtgat gccgaacgcc
     5641 tgggcctgcc gctcgtcggc acgcacctcg tcggcgaagc ggtcggagtc cagtaccgcg
     5701 cgggcctcgt cggcgtccag accggcctcg gtgccgatcc gcaccagcgt ctcggggtcg
     5761 ccgatgcgct cgccgtcggt caggtaggcc gtgaagaggc gttccttcac cgcgtcctgg
     5821 atgccgcgct cgcctgcgag gtggatcagc cggtgcgcgt cgaacgtgtt gccaccgcgt
     5881 gcgcgctcga agtggaactc ccagccgtcg gcggcggccg tctcggtcat gcgacggtgc
     5941 atgccgtcgg cctgctcctg cgagacgccg tacttcgtgg ccaggtgctc acgcaggtcg
     6001 cccgcccgct cccgcggcgc acccgggtcg agctcgaagc tccgccaggt cacctcgacc
     6061 tcgtcccggt gcgcgaactg tgcaagcgcc atctcgaacc ggcgctttcc gatcgcgcac
     6121 cacgggcaga cgacgtccga ccagatctcc accttcatgt ggacctcaac ggctcgtcac
     6181 ggggacttgt tccagtgcac tccgcgcaac gacggtcacc gcccggccgt ccaccagcac
     6241 cagtaccggc gcgtccccgg cgagccgggc cctggccgca gcgaggttct cacccgcgcc
     6301 taccgccacg ggcgcgggcg ccgcgtgggc ggtcacgtcg tcgtcggagc gggcggggtc
     6361 ggtggccacg agcgcgcgca gtgcagcggg ctcgaccacc ccgatcacct cgggtgccga
     6421 ccgggtgggc gtgtcggagt cgcgcggcgt gacgacggcg accaacccgt cggagtggat
     6481 gtcgagcacc gccgcgaggc tatgcccggc gtgcgcgacg gcgagcccgt gctcctgcac
     6541 cacgtcccgc acgaacgggc cgtgcggagc gtcgaggaag cccagctgcg tcatccagtc
     6601 gtcgtcgaag tacttggaca gatagctgcg tccggagtcg ggcaggatcg cgaccacgac
     6661 gtgctcgggg ccgagctcgg ccgctacccg cagcgccgcg gccaccgccg tgccggccga
     6721 gccgccgacg agcagcccct cctcgcgggc gagcctgcgg gccacggcca gcgactcccg
     6781 gtccgggatg cgctcgaacc ggtcgatcac gccctggtcg aacgcctgcg gccacacgtc
     6841 gtccacggtg gagggatgca ggtagtgccc gatgctctcc acgaagtacg ggctgccgtc
     6901 acccccggag tacaccgagg tgagcggatc ggccgcgaca acggtgacgc cgtgctgctt
     6961 gaggtaccgc ccggtgccgc tgatcgtgcc gccggtaccg acgcccgcca ccaggtgcgt
     7021 gacccgtccg tcggtctgct cccagatctc cggcccggtc gtcagctcgt gcacacgcgg
     7081 gttggccggg ttgtcgtatt ggtttgcgtg ccacccgccg ggtgtctcgg cggcgatgcg
     7141 gcgggcgagg ttgttcacgt gctcgggatg ttcgcgcggc agctttccca cagtctggac
     7201 gacctgcgca ccgtaggccc ggagcagggc gaccttctcc cgcgcgatcg tgtcgggcac
     7261 caccacgatc gcccggtgcc cgtgctgggc cgccaccatc gcgagcccca cgcccgtgtt
     7321 gcccgacgac ccctcgacga tcacgccgcc cggctgtagg tggccgagac gctcggcctc
     7381 tcggaccatc gccagagcgg cgcggtcctt gacactgccg ccggggttgg cgaactcgac
     7441 cttgacgtgg acggctgccg cgattcctgc ggtgatccgg ttgagctgga tgagcggggt
     7501 gccgccgacc gcgtcgagca cggatctgtg aactgtcacg gtgctccatc ctcgcccatc
     7561 cggccgtgcg gttccgggct ctgtgatccc cggctgacaa tccggtaccg catcggcggc
     7621 tcgtagcctg gcacccgtgc ccgacctacc ccgcgattgg aaccagtggc gtgaacagat
     7681 cgacctcgac cgctacgacg agcgctggcg gcggatggca gctgcgggcg gaaacccgca
     7741 cggcgaggcg gatctcatcg agtcctacca gccgggctcc gtgctcgacg ggggctgcgg
     7801 cacgggacgc gtcggcatcg agctcgcccg tcgcgggatc gcggtgctgg gcgtggacgc
     7861 cgacgcggcc atgatcgccg ctgcgcaggc caaggcgccc gacctggagt gggtggccgt
     7921 cgacctggcg gagctcgcac gaccggagcg gttcgacctc gtcgcgctcg ccggcaacgt
     7981 cgtcccgtat gtggcgccgg accggcgccg cgatgccgtt gcggcctgtg ccgggcacct
     8041 ccggccgggc ggccggctcg tcgcggggtt ccagctgcag gacgggtggc cgacgctcgg
     8101 cgactacgac ggctggtgca gggcggcggc gctgaacctg gaggatcggt tcgcgacctg
     8161 ggaccgggag ccgtatgcgg gcggcgctta cgcggtctcg gtgcacaggc tctgagcgag
     8221 ggcctgcggc gcatcggttg acctgcgggt gacggacacg gccacgtcta cccgggcgac
     8281 tcgcgggcgg at
//
LOCUS       DASRSC010000033        12203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5250829,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000033 DASRSC010000000
VERSION     DASRSC010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12203
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5250829"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VFE39_01540"
     CDS             <1..804
                     /locus_tag="VFE39_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HET6257311.1"
                     /translation="PPRAFARDHYQAQASPRTLLAVNARHPPADPGGNNRDSLISGPV
                     HGPLPILLLVLTCATGLVDAVSVLGLGRVFVANMTGNVVFLGFAAAGVPGFALAASLA
                     ALAGFLIGALAGGRLVARRRSRRGTLLRDSAVIELVLLLLGVALLAITPGTPGSAVAS
                     MVAALAAAALGLQNTTVRHLAVPDLTTTVLTMTLTGIAADIRSARRPVVTRRILAVGT
                     MFLGAVAGGLLVLHVRLAWALAPVPVLLAAVSSVLTVLLRGHPSWQDAP"
     gene            complement(928..1206)
                     /locus_tag="VFE39_01545"
     CDS             complement(928..1206)
                     /locus_tag="VFE39_01545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HET6257312.1"
                     /translation="MTPIMYQAASDSLAVFATKGGADTHPDWYRNLLAHPDVTVEVGT
                     DTVRVHARDLPAAERAPIWEKQKSSYPGFADYEAKTDRTIPVVLLERR"
     gene            complement(1325..1726)
                     /locus_tag="VFE39_01550"
     CDS             complement(1325..1726)
                     /locus_tag="VFE39_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257313.1"
                     /translation="MLGSGHQHWATVHVADLADLFRRVVENDRARGRYVAADGQNPTV
                     AALTEATAVAVQAPGAVAGSDDEARARLGEYLAEVLLLDQATTATRARSEIDWSPSRQ
                     GLVEEFRSGSYRARETNVHDDPPRVQNGRSE"
     gene            complement(1816..2271)
                     /locus_tag="VFE39_01555"
     CDS             complement(1816..2271)
                     /locus_tag="VFE39_01555"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET6257314.1"
                     /translation="MRIALTGATGFIGSHVLTELQEHGHEVTALVRDEARADLVKARG
                     ATATVVDLHDRAAVVNLLRTADGAIHTASPGDATSAEVDSAVVDAAATAYEGTGKPYL
                     HTSGIWIYGNNSSISEESPLRAPTLVAWKEPIERRVLDLRDARGTPGES"
     gene            2560..5202
                     /locus_tag="VFE39_01560"
     CDS             2560..5202
                     /locus_tag="VFE39_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6257315.1"
                     /translation="MVAPATPGLIDREDLVAALDRAATRRVTTISAPAGSGKTSLLRA
                     WAARAGRGPVVLVQVPRGQPDAQAFWLSVQGALHGARAPSGGAPAPVGTPEFDGDAMV
                     ERVLAELADLTNAGGEVTVILDDLHELGPPEASAQLRHLLGDLPPGVHAIVATRRDLR
                     LGLHRMRLDGELGELRATDLQFTDPEVRRMLAAAGIRLSDAGVARLRQRTEGWAAGLR
                     LAVISLTGDPDPERFVADFSGTDRTVAEYLLAEMLERQPADVQQVLLRTSILERVNGE
                     LADLLTGRPGAERVLLDLEDANAFVVSLDPARTWFRYHHLFADLLRLELRHTLPEQVP
                     ALHRGAAEWFVEHGQIAEAVRHTQAAGDWDAAARLLADHAFSLTLDGQAQTIEALLLA
                     FPPGASVHRADLAIAQAASDLAQGRLDQAADYLTLAENHVATTPLERRSRRRVAIASV
                     KVSLARRRGSLTGVREQVDVLTGPMSRQSNDDLALSTDLRAAALMNLGIVEAWSTGTP
                     EAERHLREGAELARENGRPYLEVACLAQLAFASKIHSFATTRRLCHEAIALAERYGWG
                     GEPIVAPALLTLAVAMAWTGEFAASAQWLDRTERALRSDTGPGIGLLRHIGTGMLHAG
                     RGDLLRALEEFGAAEHLRAQLEGSHALASQVTGWLIATQARLGTTGQARSTLGELDDE
                     RAQSGEVQNARVAISLAEGDPAAALLAVTPVLDGSAPVVGYLTVIEAHLLAAVAHREL
                     GDHRAANQAAETALALAEPDRVILPFVTTAAGDVLEALPRHETAHAALVNDILDVVHG
                     TAVAPPEPDSADTEHLSPTELKVLRYLPTNLSRPEIADELSVSVNTVNTHIRNIYAKL
                     HSGDRSTAVRRARQQRLLGAGRPR"
     gene            5262..5507
                     /locus_tag="VFE39_01565"
     CDS             5262..5507
                     /locus_tag="VFE39_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257316.1"
                     /translation="MEAVPVPYVIEINGHLGATVLAAFPGVTSHRRESHTVLVGLLDH
                     SALHGLLAQVDALGLELLELRRFAPDAGLDREAGLDG"
     gene            complement(5556..5891)
                     /locus_tag="VFE39_01570"
     CDS             complement(5556..5891)
                     /locus_tag="VFE39_01570"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6257317.1"
                     /translation="MWPACPTSTAPCDAPTSPWSITAPLQVSLICRSSRWRWARWPDP
                     AALAQVATELSATPAQVALAWLLMRSPWLLPIPGTSDPGHVEENVAAGALHLPEWARR
                     RLNEAGRPV"
     gene            complement(5830..6423)
                     /locus_tag="VFE39_01575"
                     /pseudo
     CDS             complement(5830..6423)
                     /locus_tag="VFE39_01575"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(6719..6933)
                     /locus_tag="VFE39_01580"
                     /pseudo
     CDS             complement(6719..6933)
                     /locus_tag="VFE39_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636539.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6902..7804
                     /locus_tag="VFE39_01585"
     CDS             6902..7804
                     /locus_tag="VFE39_01585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6257318.1"
                     /translation="MTTSARRRVFIGGLLLFSAASLLGGFATSQWWLLAARAIQGAGG
                     AVIAPTALALITSNFPEGRPRNRAFAVYSAMAGAGGAVGLLLGGVLTTYVSWRWVLFV
                     NLPIGLVVAAAAPRVLAASPRRPGRIDVAGAVTGTVGLALLVFGLSEAATGADGVSHW
                     GDAAVLTSLAAAAVLLALFVLIEMHSTHPLLPLRILADRNRSGAYLVMLCIGTATFGL
                     FFFLSLFVQTVLGYSPIQAGIAYLPLSVGIVAASGLASRLVTRTGPRPVILVGAAAVA
                     GGTFWFSRMTEQAEYSASCSARCC"
     gene            7795..8268
                     /locus_tag="VFE39_01590"
     CDS             7795..8268
                     /locus_tag="VFE39_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257319.1"
                     /translation="MLLTSCGLGLVFVPLSLVALHNVADRDSGVAASVLNTGQQVGGA
                     IGLAALGTIAWTTVADSVRTTSAAIAAGAQPAQAPAQVAPHALATGFSRGFLAATGIA
                     LLALLIAIATIRVSRQELSGAVPEPRQPATPQPATMQHDEDRAALAVAIRPCRYC"
     gene            8474..9391
                     /locus_tag="VFE39_01595"
     CDS             8474..9391
                     /locus_tag="VFE39_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6257320.1"
                     /translation="MSTQQQQDLDAILRQSALPVGSNVDEQRRLLRELTSAQPLPDDV
                     TVTPGMLGGVPTAEVTIDSIDPRQVVLYFHGGVYVIGDAFLAAELASQLGRRTRARVV
                     SVDYRLAPEHPYPAAVDDALAAYEALLRDGTAPSDIAFAGESAGAGLAVATLVNAREH
                     GLPLPASALAMSPYVDLTLAGRTLDTKRAVDPLLSRELLRPRVEDYTSGHDAALGLIS
                     PIFADLSGLPPLMIQAGSHEVLLDDAVRLARQAATVDVDVTLAITPRVPHVFQAYHTI
                     LDEAVAALDRAGRFLAARLAGAEPVAVPR"
     gene            9435..10361
                     /locus_tag="VFE39_01600"
     CDS             9435..10361
                     /locus_tag="VFE39_01600"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HET6257321.1"
                     /translation="MTTRDATSRRPIAVVGATGLQGGATIRALLGVDVPARALARRAD
                     SDPARALTELGADVVTADLDDPGSLRAAFRGVNGIFAMTTPGYEQRTDLEVAHGHAIA
                     DAAAAVGVPHVVYSSVGGAERHTGIPHFDSKRDVEEYLVARGLSATFVRPVFFMDNFA
                     QFATPAVEDGTLVVRLPLPAGIPLQMIAAEDIGAVAAAAMLDPDRVAGGSIEIAGDEL
                     TGEQIAEAYQHRYDIPARYEPLPIDVLGDNADQRAMFAWFAHPPAFQADIAATRELAP
                     RTKTLQQWLAGRPGLGLTKSVPTPGSVTGGPA"
     gene            11637..11810
                     /locus_tag="VFE39_01605"
     CDS             11637..11810
                     /locus_tag="VFE39_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257322.1"
                     /translation="MSDDACRYGPRLYGLCDGLGDELSADNDQGEDVREGPTLEEIIE
                     RMRLNGLDDPEQP"
BASE COUNT         1747 a         4415 c         4243 g         1798 t
ORIGIN      
        1 ccgccgcggg ccttcgcgcg tgatcactac caggcccagg cttccccgag gacactgctc
       61 gcggtgaacg ctcgacatcc accggccgat ccgggaggca acaaccggga ctcgttgatc
      121 tcgggacccg tccacggtcc gctgccgatc ctgctgctgg tcttgacgtg tgcgaccggc
      181 ctcgtcgacg cggtgagcgt cctcgggctt ggccgggtgt tcgtggccaa catgaccggc
      241 aacgtggtct tcctcgggtt cgcggccgca ggggtgcccg gcttcgcgct cgccgcctcg
      301 ctcgcggccc tggcgggctt cctcatcgga gcgctggcgg gcggccggct cgtggcccga
      361 cgccggtctc ggcggggaac cctgctgcgc gacagcgcgg tgatcgagct ggtgctgctc
      421 ctgctcggag tggccctgct ggccatcacc cccggaacgc ccggtagcgc ggtggcttcg
      481 atggtcgccg cgctcgccgc cgccgccctc gggctgcaga acaccacggt gcgccacctg
      541 gcggtccccg atctgaccac caccgtgctc accatgaccc tcaccggtat cgccgccgat
      601 atccgcagcg cccggcgccc cgtcgtcacc cgccggatcc tggccgtcgg cacgatgttc
      661 ctcggcgcgg tcgcgggtgg tctcctcgtg ctgcacgttc ggttggcgtg ggccttggcg
      721 ccggtaccgg tgctgctcgc ggcggtctcg agcgtgctca cagtgctgct ccgtggccac
      781 ccgtcctggc aggacgcgcc ctgacgcaca gcagcgccga ccctgctccc gggagcgggg
      841 cgcgggagga cggcctggtg attaccgtgc tcgagcatgt cccccgcccg gggcggcacc
      901 ggcgccgcgc aagggttcgg ttgcgcgtca gcgccgttcg agcagcacca ccgggatcgt
      961 gcggtcggtc ttcgcctcgt agtcggcgaa gcccggatag ctggacttct gcttctccca
     1021 gatcggcgcg cgctcggcgg ccggcaggtc gcgggcgtgg acgcggacgg tgtcggtgcc
     1081 gacctcgacg gtgacgtccg ggtgcgcgag caggttgcga taccagtcgg ggtgcgtgtc
     1141 cgctccaccc ttcgtggcga acacggccag cgagtcgctg gcggcttgat acatgatcgg
     1201 tgtcacgcgt tcggtgccct accggcggcc gatgtggtgg aggagcagca gcggcgcgcc
     1261 ttcgaagacg cccccgacac gtccaccatt cgcccggaac tcgtcgatcg tctgctggtt
     1321 ccagtcactc actgcgacca ttctgcaccc tcggcggatc gtcgtggacg ttcgtttctc
     1381 gtgcgcggta gcttcccgag cgaaattcct cgacgagccc ctggcgggac ggggaccaat
     1441 cgatttcgct ccgggccctg gtcgccgtgg tggcctggtc gagcaggagg acttcggcga
     1501 ggtactcgcc caggcgcgcg cgagcttcgt cgtcggagcc cgcgaccgct ccaggcgcct
     1561 ggactgcgac ggcggtcgct tcggtgagtg cggcgacggt ggggttttgt ccgtcagcgg
     1621 cgacgtagcg gccccgtgcc cgatcgttct ccaccacgcg ccggaagagg tcagcgagat
     1681 cggcgacgtg gacggtggcc cagtgctggt ggccggagcc gagcatgatc aggttgcccg
     1741 ttgcatcacg gggcgagccg aggagagcgc caggcgtgct gcctccggcg tcgccgtagg
     1801 cggcaccgga gacgatcacg actccccggg cgtcccacgg gcgtccctca agtcgagcac
     1861 tcggcgttcg atcggttcct tccaggcgac gagcgtgggt gctcgcaggg gggattcctc
     1921 gctgatcgat gagttgtttc cgtagatcca tatcccgctg gtgtgcaggt agggcttgcc
     1981 ggtgccctcg taagcggtgg cggctgcgtc gaccacggcg gagtccacct cggcgctcgt
     2041 ggcatcgccg gggctggcgg tgtggatggc tccgtccgca gtgcggagca ggttcaccac
     2101 cgcggcccgg tcgtggagat cgaccacggt ggctgtggct ccgcgggcct tgacgagatc
     2161 ggcgcgcgcc tcgtcacgaa cgagtgcggt cacctcgtgg ccgtgctcct gcagttcggt
     2221 gagcacgtgc gatccgatga acccggtcgc accggtgaga gcgattctca tggccgaggc
     2281 cctcctgttg tgaacgtggg gacgtggtgg accggctccc ggtcagcgtg accagctcgg
     2341 cgacgtccct cgtccgagcc tgggcaccga ggcttcgctc cgggtcggtg agagttcgtc
     2401 accggatccc ggtgattcgg gccgtgccgc gggcacgccg cttctgcgac cctgagggac
     2461 gacctcacac cggcccggta gcggcgaggt gcgctgcggc tgccggtgcg agggcacgcg
     2521 agacggatgc cgggcaggcg gacgcgaggt agacccgtga tggtggctcc agcgacaccc
     2581 gggttgatcg accgagaaga cctcgtcgcg gccctcgatc gcgccgccac gcgacgggtg
     2641 acgacgatct cggcgccggc cggtagcggg aagacctcgt tgctgcgcgc gtgggccgct
     2701 cgggcaggtc gcggccccgt cgtactcgtc caggtgccgc gcggtcagcc cgacgcccag
     2761 gcgttctggc tctcggtgca gggcgcgctc catggggccc gcgctccgag cgggggcgcg
     2821 ccggccccgg tgggtacacc cgagttcgac ggggacgcga tggtcgagcg ggtgctggcc
     2881 gagctcgccg acctcaccaa cgccggcggc gaggtcaccg tgatcctcga cgacctgcac
     2941 gagctgggtc caccggaagc ctccgcacaa ctccggcatc tcttggggga tcttccaccg
     3001 ggtgtgcacg cgatcgtcgc cacccgccgg gatctgcgac tgggcctgca ccggatgcgc
     3061 ctcgacggtg aactgggcga actacgcgcg acggacctgc agttcaccga ccccgaggta
     3121 cgccgcatgc tggccgccgc cggcatccgg ctctccgacg ccggtgttgc ccggttgcgg
     3181 cagcgaaccg aaggatgggc cgcgggactg cggctcgccg tgatctccct gaccggcgac
     3241 cccgatccgg agcgcttcgt cgccgacttc tccggtaccg atcggacggt cgcggagtac
     3301 ctgctcgccg agatgctgga acgccagccc gccgacgtgc aacaggtgct gctgcgaaca
     3361 tcgatcctcg aacgggtcaa cggcgagctg gctgacctgc tcaccgggcg accaggcgcg
     3421 gagcgcgtcc tgctggacct cgaggacgcg aacgcgttcg tcgtctcgct cgaccccgca
     3481 cgcacgtggt tcaggtacca ccacctgttc gccgatctcc tgcggctcga gctgcgccac
     3541 accctgcccg agcaggtgcc cgccctgcac cggggggccg ccgagtggtt cgtcgagcac
     3601 ggacagatcg cggaggcggt caggcacacc caagcggcgg gtgactggga cgccgcggcc
     3661 cggctcctgg ccgaccatgc gttcagcctc accctcgacg ggcaggcgca gaccatcgag
     3721 gcattgctcc ttgccttccc cccgggagcg tcggtgcacc gcgcggacct ggccatcgcg
     3781 caggcggcgt ccgacctcgc ccagggacgt ctggaccagg cggcggacta cctgacactc
     3841 gccgagaacc atgtggcgac cacaccgctc gagcgccgca gccgtcgccg ggtggccatc
     3901 gcgtcagtga aggtgtcgct ggcccgacgt cgtggctccc tgaccggcgt tcgcgagcag
     3961 gtcgacgtgc tcaccgggcc gatgagcagg cagtccaacg acgacctcgc gctcagcact
     4021 gacctgcgcg ccgccgcctt gatgaacctc ggcatcgtcg aggcgtggtc gacgggaacc
     4081 ccggaggcag aacgtcatct ccgtgagggc gccgagctgg cccgggagaa cggtcggccg
     4141 tacctggagg tcgcctgtct cgcccagctc gccttcgcct cgaagattca ttctttcgcg
     4201 accacccgcc ggctctgcca cgaagccatc gcgctcgcgg agcgctacgg gtggggtggc
     4261 gagcccatcg tcgcgcccgc gctgttgaca ctggccgtcg cgatggcctg gacgggcgag
     4321 ttcgccgcgt cggcgcagtg gctggaccgg accgagcggg ccctccgctc ggacaccgga
     4381 ccgggtatcg ggctgctgcg ccacatcggg accgggatgc tccacgcggg tcggggtgac
     4441 cttctccggg cgctcgagga gttcggggct gcggagcacc tccgcgcgca gttggagggc
     4501 tcgcacgccc tggcgagtca ggtgacgggc tggctgatcg ccacccaggc ccggctcggg
     4561 acgaccgggc aggcacggtc cacactcggt gagctcgacg acgagcgggc acagtcaggt
     4621 gaggtgcaga acgcccgtgt cgcgatctcc ctcgccgaag gcgatccggc cgcggccctc
     4681 ctggccgtca cgcccgtcct cgacggcagc gcaccggtcg tcggttacct cacggtcatc
     4741 gaggctcacc tgctggcggc cgtggcgcac cgtgagctcg gggatcaccg cgccgcgaac
     4801 caggccgccg agactgcact cgccctggcc gagcccgaca gagtgatcct cccgttcgtg
     4861 acgacggccg ccggcgacgt gctcgaggcg ctgcctcgtc acgagaccgc ccacgccgcc
     4921 ctggtcaacg acatcctcga cgtcgtgcac ggcaccgccg tcgccccgcc cgagccggac
     4981 tccgcggaca ccgagcatct cagcccgacc gaactcaagg tcctgcgcta tctccccacc
     5041 aacctctcgc ggcccgagat cgccgacgaa ctatccgtct cggtcaacac ggtcaacacc
     5101 cacatccgca acatctacgc caagctccac tcaggcgacc gctcgacggc cgtacgtcga
     5161 gctcgacagc agcggctgct cggggccggc cgaccgcgct gatccatctc accagatcct
     5221 ggtgatccgg attcgccggt cggcgcccga cgatgtcagc catggaagcg gtgccagtcc
     5281 cctacgtgat cgagatcaac ggacatctcg gcgcgacggt cctggccgcg ttccccggcg
     5341 tgacgtcgca ccgacgggag agccacaccg tgctcgtcgg cctgctcgac cattcggcgc
     5401 tgcacggtct cctcgcgcag gtcgacgccc tcggcctcga gttgctcgag ctccgccggt
     5461 tcgcaccgga cgccggcctc gaccgcgagg cgggcctcga cggataggac gtcccgtgcg
     5521 ggatgctccg gccgaaccgg cgtcggcgga ggagatcaga cggggcggcc ggcctcgttc
     5581 agtcggcgac gggcccactc cggcaggtgc agtgcaccgg cggcgacgtt ctcctccacg
     5641 tgtccgggat cgctcgtgcc cgggatcggg aggagccacg gcgaacgcat gagcagccac
     5701 gcgagggcga cctgcgccgg ggtggcgctg agctcggtcg cgacctgcgc gagcgctgcg
     5761 gggtcgggcc acctcgccca ccgccagcgg gaagaacggc agataaggga tacctgcagc
     5821 ggtgcagtga tcgaccacgg gctcgtcggc gcgtcgcaag gcgctgtaga ggttggacac
     5881 gctggccaga tcggtcatcg actgcgcgaa acggatctgc gcgaggtcga cgttgctgag
     5941 cccgatgtgc acgatcttcc cctcgtcgcg cagctcgagc atggtgccca acgcctcggc
     6001 gaacggcacc tccgtgccgt cggggtccgt gacccagcgc aggtgcacga cggggacggc
     6061 ctcgagacca agcacccgca ggtcccgctc gcagccctcg cgcagccttt ccggacgcag
     6121 ccccgggacc accccgccgt cgctagtgcg ccaggctccg agcttggtga cgaagatcag
     6181 gtcgtcgggg taggggcgga gcgcggcggc caggatctcg ttcgcgacgt cctcgccgta
     6241 gtaccaagcg gtgtcgatga cccggacccc gagctcgacc gcccgctggc agaccgccag
     6301 cgccagctcc cgatcaggcg gaggccccca cacgcccggc cccggcaggc gcatcgctcc
     6361 gaagcccatg cgcggtacga cgcggcccgc gatctcgacg acgcccgcgt cgacggcgtc
     6421 cattcccgga tgctaccgac accgtgttcg gagtatccag aacccgtagc gtccgtgcga
     6481 ttagccagga tccgacgacg acggctcacc tgacccggcg gaacggtggg tagctcgatc
     6541 accgactcgg ggaggatggc tcacatcgag cacgacgagc agcttcaacc ccgacgaggg
     6601 gccgtccgaa ggcgccggtc acgtccgccg aaggacaggg agccacgatg acctcggagc
     6661 gatccacgac cggacgcgac ggctcgccag ggctcccggc cggcggccca cagcgtcgtc
     6721 tccgtcggcc gaggatgtca ccggcgcggc cgccgagcag cagcagcccc cgaaggtgag
     6781 ggcgtaggcg ttgacgaccc actccagccc ggtgcccgag aagccgagcg cccgctggat
     6841 gtaggggagg gcgacgttga cgatggtccc gtcgagaacc accatgagct gcgcggtggc
     6901 gatgaccacc agtgccaggc gacgggtgtt catcggcggg ctgttgctgt tctcggccgc
     6961 gtctctgctc ggcgggttcg ccaccagcca gtggtggctg ctggccgcga gggcgatcca
     7021 gggagccggc ggtgcggtga tcgccccgac cgcgctggcg ctgatcacca gcaatttccc
     7081 cgaaggtcgg ccgcgtaacc gggcgttcgc cgtctactcg gcgatggccg gcgccggagg
     7141 cgcggtgggc ctgttgctgg gcggcgtcct gaccacctat gtgtcctggc ggtgggtgtt
     7201 gttcgtcaac ctcccgatcg ggctggtggt ggcggccgcc gcgccacgcg tgctcgcggc
     7261 atcgccccgc cgacccggcc ggatcgacgt ggccggcgcg gtcaccggca ccgtcggcct
     7321 cgcgctgctc gtcttcggcc tgtccgaggc cgccaccggg gccgacggcg tatcgcactg
     7381 gggtgacgct gcggtgctga catcgctggc agcggcggcg gtgctcctcg ccctgttcgt
     7441 gctgatcgag atgcacagca cgcacccgct gcttcccctg cggatactcg ccgaccgcaa
     7501 caggtcgggt gcctacctgg tcatgctgtg catcggcacg gcgaccttcg gcttgttctt
     7561 cttcctgtcg ctcttcgtgc agacagtgct cggctacagc ccgatccagg cgggcatcgc
     7621 ctacctgccg ttgtccgtcg ggatcgtggc cgcctcgggg ctggcctccc ggctggtgac
     7681 gcggaccggg ccacggccgg tgatcctggt cggcgcggcg gcggtcgccg gcgggacgtt
     7741 ctggttctcc aggatgaccg agcaggccga atattcggcc agctgctcgg cccgatgctg
     7801 ctgacctcct gcgggctcgg cctggtgttc gtcccgctgt cgctggtcgc tctgcacaac
     7861 gtggccgacc gggactccgg agtcgccgcg agcgtgctca acaccggtca gcaggtcggg
     7921 ggcgcgatcg ggctggcagc gctcggcacg atcgcgtgga ccacggtggc cgacagcgtc
     7981 aggactacca gcgcagccat cgccgccggc gcccagcccg cccaggcccc cgcacaggtt
     8041 gcgccacacg ccttggccac cggattctcc cgcgggtttc tcgccgccac cgggatcgcg
     8101 ctgctcgccc tgctgatcgc gatcgccacc atccgggtca gccgccagga actgtccggt
     8161 gcggtgcccg agccgcgtca gccggcgacc ccgcaacccg cgaccatgca gcacgacgag
     8221 gaccgagccg ccctcgccgt agcgatccgt ccgtgccggt actgctagcc gacaccgcac
     8281 gacctgaccg cctcctgccg gacccgggtc cggggccgac cacggcggaa tcaccagcac
     8341 taggcgagac ctcctcacct gtccggcgac gacactccat gcggcatgca tcggccgccg
     8401 gaatgccgca gcggaggtgc ggggagcgcc ccgcagcact gccacaccaa ccccgagggg
     8461 agggagctcc gccgtgagca cccagcagca acaggacctc gacgccatcc tccgacagtc
     8521 cgccctaccg gtcggcagca acgtcgacga gcaacggcga ctgctccggg agctgacgtc
     8581 ggcgcagccg ctgcccgacg acgtgacggt gaccccaggc atgctgggcg gcgtcccgac
     8641 ggccgaggtc acgatcgaca gcatcgatcc tcgccaggtc gtcctgtact tccacggcgg
     8701 cgtgtacgtg atcggggacg ccttcctcgc tgcggagctg gcctcacagc tcggcaggcg
     8761 gacgcgcgcc cgggtggtct cggtggacta ccggctcgcc cccgagcacc cgtacccggc
     8821 tgcggtcgac gacgccctcg ccgcctacga agccctcctc cgcgacggca cggccccatc
     8881 ggacatcgcc ttcgccgggg agtcggccgg cgccggcctc gccgtcgcga ccctggtcaa
     8941 cgcccgcgag cacgggttac cgctgcccgc ctccgccctc gccatgtcgc cctacgtcga
     9001 tctcaccctg gcgggcagga ccttggacac caagcgagcg gtcgacccgc tgctcagccg
     9061 ggagctcctc cgaccgcggg tcgaggacta cacctcgggc cacgacgccg ccctcggcct
     9121 gatcagcccg atcttcgctg acctctccgg cctgccgcca ctgatgatcc aggcgggaag
     9181 tcacgaggtc ctcctcgatg acgcggtccg cctggcgcgc caggccgcga ccgtcgacgt
     9241 cgacgtcacc ctcgccatca ccccgcgggt gccccacgtg ttccaggcct atcacacgat
     9301 cctcgacgaa gcggtcgccg cgctggaccg agccgggcgg ttcctggcgg cgcgtctcgc
     9361 cggcgcggaa cccgtcgccg tcccgcgatg agcaggcccc ccgaatgacg acggacccga
     9421 cggacggagg aatcatgaca acccgggacg cgacatcgcg gagaccgatc gcggtcgtcg
     9481 gtgcgaccgg cctgcagggc ggggccacca tccgcgccct tctgggcgtc gacgtgccgg
     9541 cccgggcgct cgcgcgtcgc gccgactccg acccggcccg ggctctgacc gagctcggtg
     9601 ccgacgtcgt cacggccgac ctcgacgacc ccgggagtct ccgcgcggcc ttccgcggcg
     9661 taaacgggat cttcgccatg accacccccg ggtacgagca gcgcaccgac ctcgaggtgg
     9721 cccacgggca cgcgatcgcc gacgcggcgg cagcggtcgg ggtcccgcac gtggtgtaca
     9781 gctcggtcgg cggagcggag cgccacaccg gcattcccca cttcgacagc aaacgcgacg
     9841 tcgaggaata cctggtcgcg aggggtctgt ccgccacctt cgtgcgaccg gtgttcttca
     9901 tggacaactt cgcacagttc gcgactccgg ccgtcgaaga cgggacgctg gtggtccgct
     9961 tgcccctacc ggccggaatc cccctccaga tgatcgccgc ggaggacatc ggcgccgtcg
    10021 cggcggctgc gatgctcgac cccgaccggg tcgcgggcgg gtcgatagag atcgccgggg
    10081 acgaactgac cggcgagcag atcgccgagg cctaccagca ccgctacgac atcccggcac
    10141 gatacgaacc gctgccgatc gacgtgctcg gcgacaacgc cgaccagcga gcgatgttcg
    10201 catggttcgc ccacccaccg gcgttccagg ccgatatcgc cgccaccagg gagctcgcac
    10261 cccggacgaa gaccctgcaa cagtggctcg cgggccgtcc cggtctgggg ctcacgaaaa
    10321 gcgtgccgac cccgggatcg gtgaccggtg gtccggcatg agcagcagtc caattccgac
    10381 tcgtacggcg gcaccgacgt cctcgacgtg cgcgacctgc cccgcactct tccgggagtc
    10441 ggtgaagtcc tcgccgaggt cagggcgacc gatgcaacat cagtaaggcg gtcctccgga
    10501 gcggcgcgct gcacgatctg ttgccgatga cgttcccgtc cggccaaggc agcgccgatc
    10561 gcgggcgtct tcggcccgga ccgcgcgcgg gaacgtctac cggcagctcg agcggcgccg
    10621 cacccgcgca agctggtccc gcgagggtga gtgctactcg atccgagcat ggcgcgggcc
    10681 ccgcccctcg ggcccggtcg accggtccga cgaagacccc gccgaccagc caaggccgga
    10741 cggcgggtga ccctgccgtg gccggtcccg ggaccgtcgg cggcgcggtt cgttgccttg
    10801 cgccggtggg cggcgagcgg gccctgtgcg cggcgtgcgg tgtcactggc gttgctgggt
    10861 cacgagacgg cagacagtcc gaagcagcgc tcgaagctga ccgctcgacc acaagccggc
    10921 gcggagtggg cgattgtgcc ggcggtgccg gggtgagcct cggatctcgg cggtacgaac
    10981 gatagcgtcg gccacgatct gggcaggcgg cgacgtaagc tgcagcatcg ggacccggga
    11041 atgttgacaa tgaaccggtg taggaacctt catcatcagc ttctcggggt ccgccctacc
    11101 gtagcgatac gaccccacct ccccggccgg tcacgcactc agctgcactc caattccgga
    11161 agggcctgac aactcgctca tcgcgtcata cctggcacat cctgatcaac gcgcgtcccc
    11221 cagttcgatg acaacctcgt ctcgaactgg ggctgtgcag ccgacgaccg ccggtgggtc
    11281 cctctttcgg gcgccgacaa ccacctggtt gccagctcaa ctactgggat aggcagcgcg
    11341 ctttggcgag atactcgtgt gcgtctcggt cgcatggcat ggctagacgc tgcgaccggg
    11401 cagcgggccc cgcgctctga tccgcccccc ggaccgggag cggggcccgt ccactcctcc
    11461 tgcaacagca acagcaccac cggccagacc acgactacga gcccggtgac caacggcggc
    11521 gggaaccagg cgcccgggca gcagccataa accgccaccg acccagaggc agccaaacca
    11581 accggcgggc ttccatcgcg gagcgaggta ccgatgcaca cgtccgaggg cgcaggatga
    11641 gcgacgacgc gtgtcgatac gggccgcggc tctacggact gtgtgacggc ctcggggacg
    11701 agctgtcggc cgacaacgac cagggcgagg acgtgcgcga gggcccgacg ctcgaggaga
    11761 tcatcgagcg gatgcggcta aacgggctcg acgaccccga gcagccttag cgcgaaggac
    11821 gacgccggcc agcggtacgc gcgtcgattt ggtggtcgag agttcgtcgt tgatacgtac
    11881 gccagtgagc ctccgacgcg gacagcgtgt gacgctccgt gcgccgcggt cgtgcgcgtc
    11941 gccagtactt cacccgattg gtcgcagagc ggcccacgca gggcgagcac actcttgaca
    12001 cggatcagac ggggccgtcg gggggcgccg ctacggcagg gagggccgtc ggagtggcga
    12061 cgacgcgggc ttggcgaagg gctggctggg cccgccggct aggacgtgtc tcctgattca
    12121 tgcgcgggtc cagaggatga ggctggcgag taggacgcct ccgcggtagt tgcgggcggt
    12181 cttgtcgtag cgagtggcga tcc
//
LOCUS       DASRSC010000034         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4596135,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000034 DASRSC010000000
VERSION     DASRSC010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4596135"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="VFE39_01610"
     CDS             complement(<1..586)
                     /locus_tag="VFE39_01610"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HET6257323.1"
                     /translation="MSDRVARLRSLVGEGRSDAPGRLVDVLGRLGLAGYGVVHLLVAW
                     LALEVAFGVPDAPADAEGAIGTIARTPGGLLALGLAAVGLFAFALWQVSAAAVGFRWV
                     HGGDRIRKRVGAVAKAIAMSALAILIADYLLDLGSTGGTTAQSVAAGVLALPAGRVLL
                     ALGAGTILVIAGAMTYTGMRRTFMGDLDVRHLGPA"
     gene            686..1312
                     /locus_tag="VFE39_01615"
     CDS             686..1312
                     /locus_tag="VFE39_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HET6257324.1"
                     /translation="MTETAIDPHGPEIVTEGTGWHEQLGDRAELDASFTAFAKTRADA
                     VRDLGHRVAAAGRAMELPGLLVRNRRFWVHNEWRGNRVVGCRAGEDLALTVTDVAALE
                     DVLSALVGAEPTDLQGPRWVLSDPAAAQREAQHRAVEDARQRAEGYAAALSGRLGTLR
                     RLSDAPTHAGPVAFASRESVGPDVRSLGLEPEPVRVTARCTASWTLLT"
     gene            1379..1945
                     /locus_tag="VFE39_01620"
     CDS             1379..1945
                     /locus_tag="VFE39_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257325.1"
                     /translation="MEIRTPRDADEPIGPPVRTTDLARRALHSAALVLGVLAVGMAVL
                     VGTAAAGHQAPAPAPGTVIVQAELLEPSPLPEVAMVNADLPTRARWTAPSGVTRTGLL
                     RAEAGLARGAQIPISVDAAGLVVDARIHIADPQLTGLTAGLSTLLACWTVLALIGAVC
                     RARLEAVDAQQWAAEWARVEPDWSGRAG"
     gene            complement(1942..2436)
                     /locus_tag="VFE39_01625"
     CDS             complement(1942..2436)
                     /locus_tag="VFE39_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HET6257326.1"
                     /translation="MRYWCMAFVLLYLVVEIVALVALGSVIGLGWTLLVLLAGSILGL
                     WLARREGLRAAQALADAVGNRRVPTAEVTDGMLVAAGGVLLFVPGLVTDLAGLLLLLP
                     PTRSLVRRRLVSAAERRAPGLRTARIRSQGPVVDGDVVDEPAPSDGHLVIESRVVPGP
                     GEAR"
     gene            complement(2521..>2738)
                     /locus_tag="VFE39_01630"
     CDS             complement(2521..>2738)
                     /locus_tag="VFE39_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET6257327.1"
                     /translation="GTPILNPGEAAILAVGAIKPTPWVVDGQLAVRTVCHLALSFDHR
                     LVDGEQGSRFLADVGALLTDPGLALTW"
BASE COUNT          422 a         1011 c          953 g          352 t
ORIGIN      
        1 cggcggggcc gaggtgccgc acgtcgaggt cgcccatgaa agtccgccgc atccccgtat
       61 aggtcatggc gcccgcgatc accaggatcg tgcccgcgcc gagcgccagc aggacccgcc
      121 ccgccgggag cgcgagcacg ccggccgcca ccgactgtgc ggtcgtccca ccggtggagc
      181 ccaggtccag caggtagtcg gcgatgagga ttgccagcgc cgacatcgcg atcgccttcg
      241 cgaccgctcc cacccgcttg cggatgcggt cgccgccgtg aacccacctg aaccccacgg
      301 cggccgccga cacctgccag agcgcgaacg cgaacagtcc cacggcggcg agcccgagtg
      361 cgagcagccc gcctggggtg cgggcgatcg tcccgatggc gccctcggcg tcggcggggg
      421 cgtccggcac gccgaaggcg acctccagcg cgagccacgc cacgagcagg tgcaccaccc
      481 cgtacccggc gagtccgagg cgaccgagga cgtcgacgag gcggcccggc gcatcggacc
      541 ggccttcgcc caccaggctg cgcaggcgcg ctacgcgatc agacatgtgc tctcagtatc
      601 cagggatggc tcatcgcaca ctcttccgca ttattgttct tttgctagat gaagagatac
      661 agcgcgatct ggtttcctgt cggtcgtgac cgagacggcg atcgacccgc acggcccgga
      721 gatcgtgacc gagggaaccg gctggcacga gcagttgggc gaccgggccg agctggacgc
      781 gagcttcacc gcgttcgcta agacgcgcgc cgacgcggta cgtgacctcg ggcatcgggt
      841 cgcggccgcg ggacgggcca tggagctgcc cgggctcctc gtccgcaacc ggcggttctg
      901 ggtgcacaac gaatggcggg gcaaccgggt ggtcggctgc cgggcggggg aggacctggc
      961 cctgacggtc accgacgtcg cggcgctgga ggacgtcctc tcggcgctcg tcggcgccga
     1021 gccgaccgat ctgcagggcc cccgttgggt gctctcggat ccggcggccg cacagcgcga
     1081 ggcccaacac cgtgcggtcg aggacgcccg gcagcgggcc gagggttacg cagctgcgct
     1141 gagcggccgg ctgggcacgc tccgcaggct gtccgacgca cccacacacg ccggtccggt
     1201 cgcgttcgca tcgcgggagt cggtcggccc ggacgtccgc tccctcggtc tggagccaga
     1261 acccgtacgg gtgaccgccc ggtgcaccgc cagctggaca cttctcacct gaggtagtcg
     1321 gggaacctct ttcgggtgct gcttaccgac gatcggctgc ataccggaca atgacgcggt
     1381 ggaaatccgc acgccgcgtg acgccgacga accgatcgga ccacccgtcc gtaccaccga
     1441 cctcgcccgc cgtgcgttgc acagcgctgc gctcgtgctc ggagtgctgg ccgtgggcat
     1501 ggccgtgctg gtcggcaccg cggcggctgg tcaccaggct cccgctcccg cccccggcac
     1561 cgtgatcgtg caggccgaac tgctcgaacc gtcgccgtta ccggaggtgg ccatggtgaa
     1621 cgcggacctg ccgaccaggg cacgctggac ggctccgagc ggtgtgaccc gcaccggcct
     1681 gctccgcgcc gaggccgggc tcgccagggg tgcgcagatc cccatctcag tggacgccgc
     1741 gggcctggtc gtcgatgcgc gtatccacat cgccgaccca cagctcaccg gtctgacggc
     1801 tgggctcagc accctgctcg cctgctggac ggtgcttgcc ctcatcggtg ccgtgtgtcg
     1861 tgcccggctc gaagccgtgg acgcccagca gtgggccgcc gagtgggcca gggtcgaacc
     1921 ggactggagc ggccgagccg gttagcgcgc ctcgcccggg cccggcacga cccgactctc
     1981 gatcaccagg tgcccgtcgg agggggccgg ctcatccacc acgtcgccgt ccacgaccgg
     2041 tccctgcgac cggatgcgcg cggtccgcag ccctggagcg cgccgctccg cggcgctgac
     2101 cagtctccta cgcaccagcg accgcgtggg cggcagcagg agcagcaacc ccgccaggtc
     2161 ggtgaccagg cccgggacga acagcagcac accgcccgcg gcgaccagca tcccgtcggt
     2221 gacctcggct gtcggcaccc gccggttgcc gacggcgtcg gcgagcgcct gcgccgcgcg
     2281 cagtccctcc cgacgggcga gccacaggcc caggatcgat ccggccagca ggacgagcag
     2341 tgtccagccc aacccgatca cagatccgag cgcgacgagc gcgacgatct cgacgacgag
     2401 gtacagcagg acgaacgcca tacaccagta acgcacgaga tggcctccgt gctcccgaag
     2461 acgtggtcac ggatcggtta cccgtgcgac gggcaccaca tcctcgcgga gcacggcaat
     2521 tcaccaggtg agagcgagcc ccggatcggt gagcagcgcg ccgacgtcgg ccaggaaccg
     2581 ggagccctgc tcaccgtcga ccagacggtg gtcgaacgac agcgcgagat ggcagaccgt
     2641 gcggaccgcc agttgaccgt ccaccaccca cggcgtgggc ttgatcgcac ccacggccag
     2701 gatcgccgcc tctcccgggt tgaggatcgg tgtgccgg
//
LOCUS       DASRSC010000035        12074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2958531,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000035 DASRSC010000000
VERSION     DASRSC010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12074
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2958531"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VFE39_01635"
     CDS             complement(<1..263)
                     /locus_tag="VFE39_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019807325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HET6257328.1"
                     /translation="MLFAFSIAPAGGDTGTDSVGDVVAEAVRVVRESGLPNETTSMFT
                     TIEAETWDEGMDVVKRAVEAVQAHAPRVSLVLKADIRPGHTGQL"
     gene            337..840
                     /locus_tag="VFE39_01640"
     CDS             337..840
                     /locus_tag="VFE39_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6257329.1"
                     /translation="MATRDPLPFDPILRAAAIWDERIGPSRTMAAVTSVMRVQQILLS
                     AVDGALRPHGLTFARYEALVLLTFSRTGRLPMRVMGERLQLHPTSVTNIVDRLQADGL
                     VRRIPHPTDRRATLVEITDTGSDLLEKATKSVTAIDFGLTGLSPEQETELTELLGKVR
                     RAAGDFD"
     gene            1218..2132
                     /locus_tag="VFE39_01645"
     CDS             1218..2132
                     /locus_tag="VFE39_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET6257330.1"
                     /translation="MRGRILLATSVLAALLVTACTTVVLLLVEPTGSKAEAIKTGGLA
                     GGAVVALYALWLNDRRRRTEEARHELESEKVADERFARSVELLGNEADQVRVGALHAL
                     AWLAESSPRYKQTVLDVLCAYLRRPFIHPSYTLREDDPDQAPTEDAPDADLERQVRLT
                     AQRLITDLLPWGMNRDPRAYHLDLTGAHLEYFRLEGRRIGRLTARRARFYGITKLGGV
                     EASKPALFTGAAFLGPVNMHDSRFDGGISFQHTAFCGEVALGGASVATFIDVDTTPPE
                     QHSGALTVAEGSRVAGDPARWPLACPPS"
     gene            2105..3250
                     /locus_tag="VFE39_01650"
     CDS             2105..3250
                     /locus_tag="VFE39_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6257331.1"
                     /translation="MAARLPTVMTGGVGPTGLRAARAARGLSQADAAGELAALGRATG
                     TPVAAATSLKTLLSRWENGHALPEPHYRSLLAELYGRTPTDLGIAGPQEAEGTAPAPA
                     RLRAALAAAAAVNDTGVELWWEQLTVAGRLDDELGVAGAGELVRALVEQLDETLTHTL
                     VTARRADVATVLAGAAALAGAQELDRGRPDQAWRRYDRARTAAREAGLSEAVAVAGQA
                     AVLVDVGEPGAAVELLDRAGPATSDPAQVRLDAARAVARAEAGDAAAARGAIAAAGRR
                     LQRTPVDVVAPRGGPVVELADLHRWHGRTLVALGDTAAVEPLEQALAAAPRSARHRAT
                     VHADLALALAADRPTDAAEHARRARELALRIGSAQVSARLATLGGRS"
     gene            complement(3550..4551)
                     /gene="meaB"
                     /locus_tag="VFE39_01655"
     CDS             complement(3550..4551)
                     /gene="meaB"
                     /locus_tag="VFE39_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET6257332.1"
                     /translation="MARRAGVPDLVDRARRGEQLAVARLISLVEDGAGMRFATPAGRE
                     PDVLREVAAALAPYTGRAHVVGLTGPPGVGKSTSTSALVRALRARDRRVGVLAVDPSS
                     PFSGGALLGDRVRMGEHTTDDGVFIRSMATRGHLGGLAWSTPQALRVLDAAGCDVVLI
                     ETVGVGQSEVEVVELADSTVVLLAPGTGDGIQAAKAGILEVADVLVVNKADRDGADQA
                     IRDLRHMLSLGERPAEGAWTRPVVRTVAASGEGVDDLVAALDDHRAWLDAGGERDRRR
                     IARAAAEIQAISLEQVRTRIGDLRGAHGLRELAQRVLAGELDPYRAADELMASVERG"
     gene            complement(4562..5752)
                     /locus_tag="VFE39_01660"
     CDS             complement(4562..5752)
                     /locus_tag="VFE39_01660"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET6257333.1"
                     /translation="MSGSVIVAGARTPMGRLLGGLKDFSGAQLGSFAIKAALERAGVA
                     PEQVQYVIMGQVLTAGAGQIPARQAAVGAGIPMDVPALTINKVCLSGIDAIALADQLI
                     RAGEFDIVVAGGQESMTQAPHLLPKSRAGFKYGDVTMLDHMAHDGLFCAFDQVAMGAS
                     TEKFNSRYDLTREEQDVFAARSHQLAAKASANGTFAEEITPVTVPQRKGDPTVVDTDE
                     GIRGDATAEGLGKLRPAFSSDGTITAGSASPISDGAAAVVVMSRAKAEELGLTWLAEI
                     GAHGVVAGPDASLHEQPARAIARACAKEGIDVADLDLVEINEAFAAVGLVSTKQLGID
                     PEKVNVNGGAIALGHPIGMSGARIALHLALELKRRGGGVGAAALCGGGGQGDALIIRV
                     PAAS"
     gene            5891..6328
                     /gene="mce"
                     /locus_tag="VFE39_01665"
     CDS             5891..6328
                     /gene="mce"
                     /locus_tag="VFE39_01665"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HET6257334.1"
                     /translation="MGEHTVIAVDHVGIAVADLDEAIDWYATTFGLVATHVETNEEQG
                     VREAMLSAPGDAGAAVQLLAPLRPDSAIGKFLDRSGPGIQQMAYRVADIEATCAALRE
                     KGMRLLYDEPRRGTADSRINFVHPKDAGGVLVELVQPATDNGH"
     gene            6427..7758
                     /gene="ccrA"
                     /locus_tag="VFE39_01670"
     CDS             6427..7758
                     /gene="ccrA"
                     /locus_tag="VFE39_01670"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HET6257335.1"
                     /translation="MQEILDAILADQHDALGGLDVPEHYRGVTVHADETEMFAGIPTR
                     EKDPRKSLHLDEVPTPEPGPGEALVAVMASAVNYNTVWTSIFEPMPTFGFLQRYGRLS
                     PLTKRHDLPYHVVGSDLAGVVLRTGPGVNLWKPGDQVVAHCLSVELESPDGHDDTMMD
                     PQQRIWGFETNFGGLAELALVKSSQLLPKPRHLTWEEAASPGLVNSTAYRQLVSRNGA
                     NMKQGDTVLIWGASGGLGSYATQFTLNGGGIPVCVVSSPAKAAICRKMGAELIIDRSA
                     EGYRFWKDEHSQDAKEWKRLGARIRELTGGDDPDIVFEHPGRETFGASVYVARRGGTI
                     VTCASTSGYEHTFDNRYLWMNLKRIIGSHFANYRESYEANRLIDKGLIHPTLSKVYPL
                     AETGQAVTDVHTNSHQGKVGVLALAPAEGLGVSDPEKRAKHLDAITRFRGV"
     gene            7845..9017
                     /locus_tag="VFE39_01675"
     CDS             7845..9017
                     /locus_tag="VFE39_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257336.1"
                     /translation="MAGDPILPSTDTGFEVVRRGYDQGQVETHLRTLDAHISILVTDR
                     DAALEQSAQLARDLQEARVSTEKLRAQVRTLVSPGQTVQGMSERMRAMLRLAQDEVAE
                     MLSRADTEASRRITEAEGQEAQIISAARTEAAAIVAKAKAEAERMAHDLAQARTELNV
                     ERTMAEEQLAAERREAEQAAAAEAAKAEQDRARAVAEAEAHRALVEAEAHQALVEAEA
                     HRALVEAEAHQALAEAEAHRALVEEDFTLAMEQRRAEAMAAISAERQAARREVEELRE
                     AAAARAREEDARAKERGRAIVADAERRLAELVEARKRISEQLGDARVVLDRALTSLSG
                     PHDHLAGNGNVPTPRDHGSDDGAAFDEARSSDTGEVTDASRPHPRRRRVRASVGRR"
     gene            complement(9028..9234)
                     /locus_tag="VFE39_01680"
     CDS             complement(9028..9234)
                     /locus_tag="VFE39_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257337.1"
                     /translation="MLITVAVAGLAVLVAIALIVGRVDVLARNGAWKRIAAARHAVWL
                     DEQQLRALLGQPRCVDCPMNQIDR"
     gene            complement(9713..9913)
                     /locus_tag="VFE39_01685"
     CDS             complement(9713..9913)
                     /locus_tag="VFE39_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257338.1"
                     /translation="MVRNVPGSSSAKTYRCPGCDHLIPAGTPHVVAWPAAEFGSVEER
                     RHWHAACWSARDRRGPGRRRWR"
     gene            complement(10039..11823)
                     /locus_tag="VFE39_01690"
     CDS             complement(10039..11823)
                     /locus_tag="VFE39_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HET6257339.1"
                     /translation="MARVFRTVGVVGLGTMGAGIVEVFARNGLNVVAAEVDLEAVERG
                     RTHLETSTARAVGRGKLTREDADALLARIRTTTSLADLADADLVVEAVPESLDLKATL
                     FAELDKVCRPETILASNTSSLSVTKLAVRTGRPGKVIGMHFFNPAPVQKLVEVVRTVV
                     TEQDVVDDVKAFAATLDKVPVVIGDRAGFIANALLFGYLNHAARMYEARYASREDLDA
                     AMRYGCGYPMGPLALLDLIGLDTSFEILDTMYRESRNLMHAPAPVLRQMSTAGLLGRK
                     TGRGFYTYAKPHSSEVVPDALTPSGALPEEVAVRDVQRVGVVGTGTMASGIVEVFARS
                     GLDVIVRGRSISKAEGSVAAIRKSLEKGVVRGRLTESERDETLARITATTRLEDLADV
                     DLVVEAVAEELDVKRAVFAALDEICKPGAILATTTSSLPVVECAAATSRPHDVVGLHF
                     FNPAPLMKLVEVVSTITTAPEVTAAARALCERLGKVPVSCGDRAGFIVNALLFPYLND
                     AVKMLEVHYATADDIDAAMKTGCALPMGPFELLDVVGLDVSLAIQRSLYQEFRLSGLA
                     PAPLLEHLVTAGRLGRKSGHGFRDYAAR"
BASE COUNT         1850 a         4443 c         4180 g         1601 t
ORIGIN      
        1 agctgcccgg tgtgacccgg ccggatgtcg gccttgagca ccaggctcac ccgcggagca
       61 tgggcctgca ccgcctcgac ggcgcgtttc accacgtcca ttccctcgtc ccaggtctca
      121 gcctcgatgg tggtgaacat cgaggtggtc tcgttgggca acccggactc gcggacgacg
      181 cggaccgcct ccgcgacgac gtcgcccacg ctgtcggtgc cggtgtcgcc ccctgccggg
      241 gcgatgctga atgcaaagag catggtcgac agcctgccgc agggaacgtg gcaaccgcga
      301 ccccgcccgg gcacgtcacc gctagcatcg ccggtcatgg ccacccgcga cccgcttccg
      361 ttcgacccga tcctccgggc ggcggcgatc tgggacgagc ggatcgggcc ctcacgcacg
      421 atggccgccg tgaccagcgt gatgcgggtg caacagatcc tcctctcggc ggtggacggc
      481 gccctgcgcc cacacggcct caccttcgcc cgctacgagg cgctcgtgct gctgaccttc
      541 tcccgcaccg gccggctgcc catgcgggtg atgggcgagc ggctgcaact gcaccccacc
      601 agcgtgacca acatcgtcga tcggctccag gccgacgggc tcgtgcgccg catcccgcac
      661 ccgacggacc ggcgggccac cctggtggag atcaccgaca ccggctctga cctcctggag
      721 aaggcgacga agtcggtgac cgcgatcgac ttcggcctca ccgggctcag cccggagcag
      781 gagaccgagc tgaccgaact gctggggaag gtgcgccggg cggccggcga cttcgactga
      841 cgcgcggccc tcccgccacg tgcgactcgt cagggtggcg gtgggcccac cgcccctccg
      901 gcgtcgccgc cccggccgcc acccggccgc ctccgagcgc gcctgcgccc gcccggccac
      961 gagcgcatgg tgcggcggcg cgtggaccgt gtggccgccc gccgccccga tcggcccctg
     1021 ccccgccccc cggccgcgca gtagccgctg accgctccac cggccgaccc agccagcacg
     1081 cagaccgagc acgtcgtcag atccatctcc gcccctctcg accgctgttg acgcatgttg
     1141 cggtaacagc gttgacagta ggcaccggcc ccgacactct gaacgacgac ggggagatca
     1201 acgggcgagg agctgacgtg cgaggccgga tcctgctggc gaccagcgtg ctcgcggccc
     1261 tcctcgtcac cgcgtgcacc acggtggtgc tactactggt cgagcccacg gggtccaagg
     1321 ccgaggcgat caagacgggc ggtctcgccg gcggcgcggt cgtggcgctg tacgcgctgt
     1381 ggctcaacga ccgcaggcga cgcaccgagg aggcgcgcca cgagctcgag agcgagaagg
     1441 tcgccgacga gcggttcgcg cggtcggtcg aactcctggg caacgaggcc gaccaggtgc
     1501 gcgtcggcgc tctgcacgcg ctcgcgtggc tcgccgagtc cagtccccgc tacaagcaga
     1561 cggtgctcga cgtgctgtgc gcgtacctgc gcaggccctt catacatccc agctacacgc
     1621 tgcgggagga cgaccccgac caggcgccca ccgaggatgc accggatgcc gatctggagc
     1681 gccaggtgcg gctcaccgcc caacggctga tcaccgatct gctcccgtgg gggatgaacc
     1741 gcgacccacg cgcctaccac ctcgatctca ccggcgcgca tctggagtac ttccggctgg
     1801 agggaaggag gatcggccgc ctcaccgcgc ggcgcgcgcg gttctacggc atcaccaagc
     1861 tcggcggggt cgaggcgagc aaacccgcgt tgttcaccgg cgccgccttc ctgggcccgg
     1921 tgaacatgca cgactcccgg ttcgacggtg ggatctcctt ccagcacacc gccttctgcg
     1981 gcgaggtggc cctcggtggc gcgtccgtgg ccaccttcat cgacgtggac accaccccac
     2041 cggaacaaca ctccggtgcc ctcaccgtcg cggagggcag cagggtggcc ggcgacccgg
     2101 cgaggtggcc gctcgcctgc ccaccgtcat gaccggcggg gtggggccca cgggcctgcg
     2161 cgccgccagg gcagcacgcg gcttgtccca ggccgacgcg gccggggagc tggcggcgct
     2221 gggccgcgcc acgggcacac ccgtcgcggc cgccacgagc ctgaagacac tcctctcgcg
     2281 ctgggagaac ggccatgccc tgccggagcc gcactaccgg tcactcctcg cggagctgta
     2341 cgggcgcacg ccgaccgacc tcggaatcgc ggggccgcaa gaggccgaag gcacggcccc
     2401 ggcgccagcc cggctgcggg cagcgctcgc ggcggccgct gccgtcaacg acaccggcgt
     2461 cgagctgtgg tgggagcagc tgactgttgc cggcaggctc gacgacgagc tgggcgtggc
     2521 cggggccggc gagctggtgc gcgcactggt cgagcagctc gacgagacgc tcacccacac
     2581 cctggtgacc gcccgccggg cggatgtggc gaccgtgctg gccggggccg ccgcgctggc
     2641 gggagcccag gagctcgacc gcggacgccc cgaccaggcc tggcgccgtt acgaccgcgc
     2701 ccgtaccgcc gcgcgggagg ccggcctttc ggaggccgtc gccgttgccg ggcaggctgc
     2761 cgtgctggtg gacgtcggtg agcccggggc cgccgtcgag ttgctcgacc gggccgggcc
     2821 cgcgacctcc gatcccgcgc aggtgcgact ggacgccgcg cgcgccgtcg cccgcgccga
     2881 ggcaggcgac gccgcagctg cccgcggggc catcgccgcg gccgggcggc ggctacagcg
     2941 cacgccggtc gacgtcgtcg ctccacgggg tggcccggtc gtcgagctgg ccgatctgca
     3001 ccgctggcac ggccgcacgc tggtcgcgct cggcgacacg gcagcggtcg agccactgga
     3061 gcaggcactt gccgccgctc cccgatctgc ccgccaccgg gctaccgtcc acgccgacct
     3121 cgcgctcgcg ctcgcggccg accggcccac cgacgcggcc gagcacgcac gccgggccag
     3181 ggagctcgcc ctacgcatcg gctcggcgca ggtgagcgcc cggcttgcca cgctcggcgg
     3241 ccggtcctga cccgaagccc gcgctgctgc gcgcccggcg tggtcttcgc agacccacgg
     3301 gccctgcgac ggccaaggct cgcgcggctg ccggatcatg gccccggtcg atcgccgggc
     3361 gggacgacga tcaccggtcg gaacgcgaag gcgtgcccag tccggctgcc gaccccacaa
     3421 cactgatcac ggcaccggtc aactacccgc ccgggacgat gatcaccagc gcggaccgcg
     3481 caggggcgcc caccgcggct accggtctca aaacgatcac ggtcgcgagt cgttccccgg
     3541 cgtttcggct cagccccgtt ccacgctggc catcagctcg tcggcggcgc ggtacgggtc
     3601 gagttcgccg gccagtaccc gctgggccag ctcgcggagt ccgtgcgccc cgcggaggtc
     3661 gccgatccgg gtccgcacct gctcgaggct gatcgcctgg atctccgccg cggccctggc
     3721 gatccgccgc cggtcgcgct cgccgcccgc atcgagccac gcacgatggt cgtcgagcgc
     3781 cgccaccagg tcgtccacgc cttcgccgct cgcggccacc gtgcgcacca ccggccgcgt
     3841 ccaggcaccc tcggccgggc gctccccgag cgagagcatg tgccgcaggt cgcggatcgc
     3901 ctggtcggcg ccgtcgcggt cggccttgtt cacgacgagg acatcggcca cctcgaggat
     3961 ccccgccttg gccgcctgga tgccgtcgcc ggtgccgggg gccagcagca ccacggtgct
     4021 gtcggcgagc tccaccacct ccacctcgga ctgccccacc ccgacggtct cgatgagcac
     4081 gacgtcgcag cccgcggcgt ccagcacgcg caacgcctgc ggcgtcgacc acgccagccc
     4141 gccgaggtgc ccgcgcgtgg ccatcgagcg gatgaacacc ccgtcgtcgg tggtgtgctc
     4201 acccatccgc accctgtcgc cgagcagcgc gccgcccgag aacggtgatg acgggtccac
     4261 ggcgagcacc ccgacccgcc ggtcccgcgc gcgcagggcc cggaccagcg ctgacgtcga
     4321 cgtcgacttg ccgacaccgg gcggccccgt cagcccgacg acgtgggccc ggcccgtgta
     4381 cggggcgagc gccgccgcca cctcgcgcag cacgtccggc tcacggcctg cgggggtggc
     4441 aaagcgcata ccggcgccgt cctccaccag cgagatcagc cgcgcgacgg ccagctgctc
     4501 accgcgccgg gcgcggtcga ccaggtcggg aactcccgcc cgccgcgcca tgtcggtggg
     4561 gtcagctcgc cgccggcacg cggatgatca gagcgtcgcc ctgacccccg ccgccgcaga
     4621 gcgctgccgc gccgacgccg ccgccacggc gcttgagctc cagcgccagg tgcagcgcga
     4681 tgcgggcgcc ggacatcccg atcgggtggc cgagcgcgat cgcgcccccg ttgacgttca
     4741 ccttctcggg gtcgatgccc agctgcttcg tggacaccag gccgaccgcg gcgaacgcct
     4801 cgttgatctc gacgaggtcg aggtccgcga cgtcgatgcc ctccttggcg cacgccctcg
     4861 cgatggccct ggccggctgc tcgtgcaggc tcgcgtccgg tccggcgacg actccgtgcg
     4921 caccgatctc ggccagccac gtgagcccga gctcctcggc cttggccctg ctcatgacca
     4981 ccactgcggc ggcgccgtcg gagatcggtg acgccgagcc cgcggtgatc gtgccgtccg
     5041 aggagaacgc ggggcgcagc ttgccgaggc cctcggctgt ggcgtcaccc cggatgccct
     5101 cgtcggtgtc gacgaccgtg gggtcgccct tccgctgagg caccgtgacg ggggtgatct
     5161 cctcggcgaa cgtcccgttg gccgacgcct tggccgcgag ctggtgcgag cgggcggcga
     5221 acacgtcctg ctcctcgcgg gtgaggtcgt agcgggagtt gaacttctcg gtggaggcgc
     5281 ccatcgcgac ctggtcgaaa gcgcagaaca ggccgtcgtg ggccatgtgg tcgagcatcg
     5341 tgacgtcgcc gtacttgaac ccggcgcgcg atttcggcag cagatgcggg gcctgcgtca
     5401 tcgattcctg gccgcccgcc accacgatgt cgaactctcc cgctcggatg agctggtcgg
     5461 ccaacgcgat ggcgtcgatc cccgacaagc acaccttgtt gatcgtgagc gctggaacgt
     5521 ccatggggat gccggcgccg accgccgcct gccgggccgg aatctggccg gcgcccgcgg
     5581 tgagtacctg acccatgatc acgtactgga cctgttcggg agcgacaccg gcccgttcca
     5641 gcgccgcctt gatcgcgaac gagccgagct gggccccgct gaagtccttc agaccgccca
     5701 acaacctccc catcggggtg cgggctccag cgacgatcac ggatccggac acgatgacct
     5761 ccagcggctg cggtggcagg ttcaatgacg atacccgcgg cgctaccgaa ggtctcggct
     5821 ccgcacccat gggattggtg atgcctcgca caagcagccc tgcacgcagc atcccgttta
     5881 cattcccagg atgggagaac acaccgtcat cgccgtggat cacgtcggga tcgcggtcgc
     5941 ggacctggac gaggccatcg actggtacgc cacgacgttc gggctcgtcg ccacgcacgt
     6001 cgagacgaac gaggaacaag gggtgcgcga ggcgatgctg tcagcgcccg gcgacgccgg
     6061 ggccgcggtc cagctgctcg cgccccttcg cccggactcg gcgatcggga agtttctcga
     6121 tcgcagcggc ccggggatcc agcagatggc gtaccgcgtc gcggacatcg aggccacctg
     6181 cgcggcgctg cgggagaagg gcatgcgcct gctctacgac gagccgcgac ggggcaccgc
     6241 cgactcccgc atcaacttcg tgcacccgaa ggacgcgggc ggcgtgctcg tcgagctcgt
     6301 gcaaccggcg acggacaacg gccactgacg agtggcgctg gtcactctca gcacgtacga
     6361 gtaccatacc gtgcagtaac tttcgggata ccaagcaatc acgactcgag ccagcggagg
     6421 ccagccgtgc aggagatcct cgacgccatc cttgccgatc agcacgatgc cctgggtggg
     6481 ctcgacgtac cggagcacta ccggggcgtc accgtgcacg cggacgagac ggagatgttc
     6541 gccgggatcc ccacgcggga gaaggacccc cgcaagagcc tgcacctgga cgaggtgccc
     6601 acgccggaac caggccccgg cgaggcgctc gtggccgtga tggcgagcgc tgtcaactac
     6661 aacacggtgt ggacgtcgat cttcgagccg atgccgacgt tcggcttcct gcagcgctac
     6721 ggcaggctct ccccgctcac gaagcgccat gacctcccgt accacgtcgt cggctccgac
     6781 ctggccggtg tcgtgctgcg gacgggcccc ggggtcaacc tgtggaaacc cggcgaccag
     6841 gtcgtcgccc actgcctgtc ggtggagctg gagagccccg atgggcacga cgacacgatg
     6901 atggaccccc agcagcgcat ctggggcttt gagacgaact tcggcgggct cgcggagctc
     6961 gcgctcgtga agtcgagcca gctgctgccg aagccgaggc acctgacgtg ggaggaagcg
     7021 gcgagccccg gcctcgtcaa ctcgacggcc taccggcagc tcgtgtcccg caacggcgcc
     7081 aacatgaagc agggcgacac ggtgctgatc tggggtgcgt ccggcgggct cggctcgtat
     7141 gccacgcagt tcaccctgaa cgggggcggg atcccggtct gcgtcgtctc ctcgcctgcg
     7201 aaggccgcga tctgccgcaa gatgggggcg gagctgatca tcgaccgaag cgccgagggg
     7261 taccggttct ggaaggacga gcacagccag gacgcgaagg agtggaagcg tctcggggcg
     7321 aggatccgcg agctgaccgg cggggacgat cccgacatcg tgttcgagca ccccggccgg
     7381 gagacgttcg gcgcgagcgt ctacgtcgcc cgtcggggcg gcacgatcgt cacctgcgcg
     7441 tcgacgtccg gctacgagca caccttcgac aaccggtacc tgtggatgaa tctcaagcgg
     7501 atcatcggct cgcacttcgc caactaccgg gagtcgtacg aggccaaccg gctgatcgac
     7561 aagggcctca tccacccgac cctgtcaaag gtgtacccgc tcgcggagac cgggcaggcc
     7621 gtgacggacg tgcacaccaa cagccaccag ggcaaggtcg gcgtgctggc gctcgccccc
     7681 gcggaagggc tcggcgtgtc cgacccggag aaacgcgcga aacacctcga cgccatcacg
     7741 cgctttcgcg gcgtatgact gaatgtcccc cggagggtga ggccgttcac caccgagcgt
     7801 gcacatcacc aaggtccaac tcgatgcccg gtacggtgag gcccatggcc ggagacccca
     7861 ttttgccctc aaccgacacc ggcttcgagg tcgtccgcag gggctacgac cagggccagg
     7921 tcgagaccca cctgcgcacc ctcgacgccc acatcagcat cctcgtcacg gatcgggacg
     7981 cggcactgga gcagtcggcc cagctcgccc gtgaccttca ggaagcccgg gtaagcaccg
     8041 agaagctgcg cgcacaggtc cgcacgctgg tgagccccgg gcagaccgtg cagggcatga
     8101 gcgagcggat gcgcgccatg ctgcggctgg cccaggacga ggtggccgag atgctcagcc
     8161 gcgccgacac ggaggccagc cggcgcatca cagaggccga ggggcaggag gcgcagatca
     8221 tctccgccgc ccggaccgag gccgcggcca tcgtggccaa ggccaaggcc gaggcggagc
     8281 ggatggcaca cgacctcgct caggcccgta ccgagctcaa cgtcgaacgc acgatggccg
     8341 aggagcagtt ggccgcggag cggcgcgagg cagagcaggc ggccgccgcc gaggccgcga
     8401 aggccgagca ggacagggcc cgcgccgtgg ccgaggcgga ggcgcaccgg gcactggtcg
     8461 aggcggaggc gcaccaggca ctggtcgagg ctgaggcgca ccgggcactg gtcgaggcgg
     8521 aggcgcacca ggcactggcg gaggctgagg cgcaccgggc actggtcgag gaggacttca
     8581 cgctcgccat ggagcagcgc cgcgccgagg cgatggccgc aatcagcgcg gagcggcagg
     8641 cggcccgccg cgaggtggag gagctgcgtg aggcggcggc tgctcgcgcc cgcgaggagg
     8701 acgcgcgggc gaaggagcgc ggccgcgcca tcgtcgccga cgccgaacgc cgcctcgccg
     8761 aactcgtcga ggcccgcaaa cggatcagtg agcaactggg ggacgcccga gtcgtcctcg
     8821 accgcgccct tacttcgctg tcggggccgc acgaccacct cgccggcaac ggcaacgtgc
     8881 ccacgccacg tgaccacggc agcgacgatg gtgccgcatt cgacgaggcc cggtcctcgg
     8941 acaccggcga ggtcacggac gccagccgcc cgcatccccg gcgccgccgc gtccgggcct
     9001 cagtcggccg ccgttgagcg gctgacgtca gcggtcgatc tggttcatcg ggcagtcgac
     9061 gcacctcggc tgcccgagca aggcccgaag ctgctgttcg tcgagccaga cggcatggcg
     9121 ggcggccgcg atcctcttcc aggccccgtt ccgcgccagg acatccaccc ggccgacgat
     9181 caaggcgatc gcgaccagca cggccagccc ggcgaccgcc accgtgatca acatagttcg
     9241 ctgcccatga catgccccct tcgtcgaggg gcggctccgt tgtgtccctg ctaagggacg
     9301 tgggtcgcag ttccagattc cctggcagat ctgcctgccg caagcatttc tcgaaatcac
     9361 gcgcagttcc acctggacgg ctcatccttg ctgtaacgaa ttgctggaaa cctaagaaga
     9421 atgtaccgaa acggcgtagt cgccgctggt aacggacgca cgcacgcccc ctacctccgc
     9481 caggacacct cgccggggcc gactcgaaca gatctggagg ctgcacgaga tcggcagttc
     9541 cggccggagc acccgtctga tcgccggtaa cgatgagccg gcgccgcacg ggtgatcgaa
     9601 tccgatcgat aacgcgtaca acccgatcga tcaagcgatc tatccggccc ggagaaattg
     9661 atctcagcag atccaaaaat gaaatagccg aggcgttcgc ggcatcgccc gttcagcgcc
     9721 accgacgtcg gcccgggccg cgacggtccc gggccgacca gcaggcggcg tgccagtgcc
     9781 tgcgctcctc caccgagccg aactcagccg cgggccacgc tacgacgtgc ggcgtcccgg
     9841 ccgggatcag gtggtcgcat cccgggcagc ggtatgtctt cgccgacgac gaaccgggca
     9901 cgttgcgcac catccagtcg ccgtcggctc cgctctccac tcgggtgtgg gacgccgagc
     9961 gcagcgggac gtgctcgtcg cgccgacggc cgggccgtcg cggccggtgg gatctgggca
    10021 cggtggacgg aaacgacgtc agcgcgccgc gtagtcccgg aagccgtggc cgctcttgcg
    10081 gcccagcctg cccgccgtca ccagatgctc caacaggggc gccggcgcga gaccggagag
    10141 ccggaactcc tggtagagcg agcgctggat agccagcgag acatccagcc cgaccacgtc
    10201 gagcagctcg aacggtccca tcggcagcgc acagccggtc ttcatggccg cgtcgatgtc
    10261 gtcggcggtg gcgtagtgca cctcgagcat cttcaccgcg tcgttgaggt acgggaacag
    10321 cagcgcgttg acgatgaagc cggcccggtc accacaggac accggcacct tgcccagccg
    10381 ctcgcacagc gcccgggccg cagcggtgac ctcaggcgcg gtggtgatcg tggagaccac
    10441 ctcgaccagc ttcatcagcg gcgcggggtt gaagaagtgc agcccgacga catcgtgcgg
    10501 cctggacgtg gcagcggcgc actccacgac ggggagcgag gacgtggtgg tggcaagtat
    10561 cgcgcccggc ttgcagatct cgtccagcgc cgcgaacacg gcccgcttca cgtcgagctc
    10621 ctcggcgacg gcctccacca cgaggtcgac gtcggcgagg tcctccaggc gggtggtggc
    10681 cgtgatccgg gccagcgtct cgtcgcgctc ggactcggtg aggcggccgc gcaccacgcc
    10741 cttctccagg gacttcctga tggcggcgac cgagccctct gccttggaaa tggagcgccc
    10801 gcgcacgatc acgtccaggc cgctcctggc gaacacctcg acgatcccgg aggccatggt
    10861 gccggtaccg accacaccca cccgctgcac gtcgcgcacc gccacctcct cgggcagggc
    10921 acccgacggg gtgagcgcgt cgggcacgac ctcggagctg tgcggcttgg cgtaggtgta
    10981 gaagccacgt ccggtcttgc gacccagcag gcccgcggtg ctcatctgcc ggagcaccgg
    11041 cgccggcgcg tgcatcaggt tgcgcgactc ccggtacatc gtgtcgagga tctcgaagga
    11101 cgtgtcgagc ccgatcaggt cgagcagggc cagcgggccc atcgggtagc cgcagccgta
    11161 ccgcatggcg gcgtcgaggt cctcgcggct cgcgtaccgc gcctcgtaca tccgcgcggc
    11221 gtggttgagg tagccgaaga gcagcgcgtt ggcgatgaaa ccggcgcggt ccccgatcac
    11281 gaccgggacc ttgtcgagcg tggcagcgaa ggccttcacg tcgtcgacga cgtcctgctc
    11341 ggtgacgacg gtgcgcacca cctccacgag cttctggacg ggcgccgggt tgaagaagtg
    11401 catcccgatg accttgccgg gacgcccggt gcgcaccgcc agcttggtga ccgacaggga
    11461 cgaggtgttg gacgccagga tcgtctcggg acggcagacc ttgtccagct ccgcgaacag
    11521 cgtggccttc aggtccaggc tctccggcac cgcctcgacc acgaggtccg cgtcggcgag
    11581 gtcggccagc gatgtcgtcg tgcggatccg ggcgaggagc gcgtcggcgt cctcccgggt
    11641 cagcttgccg cggccgaccg cgcgtgcggt ggacgtctcc aggtgcgtgc ggccccgctc
    11701 gaccgcttcg aggtccacct cggcggcgac cacgttcagc ccgttgcggg cgaacacctc
    11761 gacgatgccc gcgcccatcg tgcccagacc gaccacgccg accgtccgga atacgcgtgc
    11821 cacgtgtcca cctcccgcta ccagggacat tcttcggatc ctcgcattcc ggcggcccac
    11881 gcgggcgggc gtgtgactgc gccaaccctt cgtgcagcca ccggcctacc gaccgtgtcg
    11941 cagccgcacg accccgagca gcgccttcat ccctcgtgag gtgcgccgga agctggtgac
    12001 ggcgatgggc agggcgtacc tctgacgtgc cacggcctgc gggcgggtga actcgcggag
    12061 cccgtcctcg ccgt
//
LOCUS       DASRSC010000036        13365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4596424,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000036 DASRSC010000000
VERSION     DASRSC010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 13365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13365
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4596424"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..518
                     /locus_tag="VFE39_01695"
     CDS             45..518
                     /locus_tag="VFE39_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257340.1"
                     /translation="MSEADGDGVAGEHRAPYRAVGGHEAAGEHKAGRGYKAGREYKEV
                     LAGITAAVDALRERDRVRAAGHARELVSLEDDMLRAGERAALTRLGVELHWEAALEAL
                     WMESWMKLRPRPDPDPRAGSGAGSADLAALDAEVEQRSAELQDAVRRRRFGFPRR"
     gene            complement(610..2526)
                     /locus_tag="VFE39_01700"
     CDS             complement(610..2526)
                     /locus_tag="VFE39_01700"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET6257341.1"
                     /translation="MRERYRSRRWPFVVILVMGMAALGLVMPSSAVAPPVGMTAPALP
                     GHPVAMEPVSPTEPQVPSAPADPRTEVSRSPESAPLPASTVSRRPKLAGVNFGRTGPL
                     VLGLTEADLPARSAGPAPPAAAAPLPAVRTPALPRITTTAKPGTAAAAAIAFAIAQRG
                     LPYVWGGDGPQSGEAGFDCSGLTTASYDYAGISLPRTAHTQYYRGPHLPAGAPLQPGD
                     LVFYGVPEAVHHVGLYIGGGRMINAPTFGEPVRTAYVRYPGDDYLGATRPAAGRDAPG
                     VLAKPDLPAEVPPVPAPDAPRAPTEFRAPPAPDVPQIAPQLAPAPVRGGEQAAPPSLA
                     GGSETPGSTAPGSTATGTMTAATPTDDPTLTAAAPSSTPTSSARSGSTPTSSTPISSA
                     DAATNSAPTSNTPADPTGSAQTGTAPTGSAPPDTTPSGNAPANSPPADSAPTDHDSAP
                     TGSAPAASAPAAGAPGDNASTSSAPARTAPTTSAPTRSAPASSASAPPPSTRASAPKG
                     AGSRPSVPQPDAAPTPTSITLPDGEVLQVVSSKMGGDGLPGPPTTPGTATLAHAGRTT
                     VVTLPQTVGTVGLATGSTIQVTNAGGDRAALDVRSRRTVDTSTAAGIASAGGHRLVLV
                     LPRDDGEVQVVVAS"
     gene            2675..4105
                     /locus_tag="VFE39_01705"
     CDS             2675..4105
                     /locus_tag="VFE39_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET6257342.1"
                     /translation="MIFTDARELARMLRAKEISAREVLDAHLAQIERCNPDVNAIVTL
                     VADRAADEAAAADERLAHGDEVGPLHGLPIAHKDTHDTAGIRTTYGSPLLADNVPTRD
                     ALVVERIRAAGAITIGKTNVPEFAAGSHTINPVFGPTRNPYAPSRSPGGSSGGAAAAL
                     ACGMHPLADGSDMGGSLRNPASYCNVVGLRPSPGRVPAWPDAAPWSTLAVPGPMARTV
                     SDVALLLGVLTGPDPRDPTALDTPGAVFAAEPPTDVRGLRVAVAPDLGGAVPVDPAVK
                     VVVERAARVLEELGCVVEPACPDLTGADEAFRTLRAWQFELAYSAMLDAHPDDLKATL
                     RDNILAGRTLTGPDVGRAHVLQAALYHRVREFFGRYDALLAPVSQVPPFPIEWEYPTE
                     VAGVPMHDYLEWMGSAYLISATGCPAISVPAGFTPDGLPVGLQIVGRHRGELGLLRIG
                     RAFEQATGHGRTRPRGAAPGDTSTSS"
     gene            complement(4343..4525)
                     /locus_tag="VFE39_01710"
     CDS             complement(4343..4525)
                     /locus_tag="VFE39_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019889383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6104 family protein"
                     /protein_id="HET6257343.1"
                     /translation="MYFTDRGIEELTERRGEEQVSVEWLADRLRTFVDQNPSFEDAVE
                     RLASFLARDDSDDLDE"
     gene            complement(4537..8409)
                     /locus_tag="VFE39_01715"
     CDS             complement(4537..8409)
                     /locus_tag="VFE39_01715"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HET6257344.1"
                     /translation="MSSSSTSNPAGQFGPNEWLVEEMYQRFLDDPESVDPAWHEFFAD
                     YRPADDTSAPTGNGVATRTAPRSSAPTSPAPTPTAEPAESTERRRVGDRKAMTPPPTA
                     SSRTGTASPNRTTSNGTTSNGTTSNGTATATGGRTAAEKTPATSSAAPTGTSDKPAAG
                     TTSQAAKPSPVSSEGRATPLRGAAGAVVKNMNASLTVPTATSVRAVPAKLLADNRVVI
                     NNQLTRTRGGKISFTHLIGFAVVQALADFPVMNRHFVETDGKPTVVQPEHVNLGLAID
                     LPGKNGQRSLVVVSIKGCEAMNFAQFWSAYEDIVRKARGGNLQSEDFAGTTISLTNPG
                     TLGTNHSVPRLMTGQGTIIGVGAMEYPAEFQGASDERLAEIGISKIITLTSTYDHRII
                     QGAESGDFLRRVHALLLGEDAFYDDIFRSLRVPYEPVRWVQDIPDGAVDKTARVIELI
                     DAYRTRGHLMADTDPLNYRQRRHPDLDVLSHGLTLWDLDREFAVGGFGGKERMKLRDV
                     LGLLRDSYCRTIGTEYMHIADPEQRNWLQERIEVPHQKPNPAEQKYVLSKLNAAEAFE
                     TFLQTKYVGQKRFSLEGGETVIPLLDAVLDKAAEHELDEVVIGMPHRGRLNVLANIVG
                     KPISQIFREFEGNLDPGQAHGSGDVKYHLGAEGKYFRMFGDGETVVSLASNPSHLEAV
                     DPVLEGIVRAKQDLLNKGEGGFSVLPLMMHGDAAFAGQGVVAETLNLAKLRGYRTGGT
                     VHVVVNNQVGFTTAPEHARSSQYSTDVAKMIDAPVFHVNGDDPEACVWVAKLAVEYRE
                     RWNNDVVIDMICYRRRGHNEGDDPSMTQPAMYDVIDAKRSVRKIYTESLIGRGDISME
                     EAEHALRDFSNQLEHVFNEVRELEKTPAAPSPSVEQEQSVPTDLDTSVPLEVVHRVGD
                     AHVELPEGFTVHQRVKPVLQRRYQMSREGNVDWAFAELIALGSLAMDGKLVRLSGQDT
                     RRGTFVQRHSVVIDRKTGAEYYPLRNLSQDQERFLPYDSALSEFAAVGFEYGYSVANP
                     EAIVAWEAQFGDFVNGAQSIIDEFISSGEAKWGQTADIALLLPHGLEGQGPDHSSGRI
                     ERFLQLCAEGSMTVALPSEPANYFHLLRRHAMDGVRRPLIVFTPKSMLRNRAVVSPLS
                     DFTGGRFRSVIDDPRFRESDGPASGVRKLLLCSGKIYYELAAARDKREIDDTAIVRIE
                     QLYPVPDRQLAAVLERYPNAEDVRWVQEEPANQGAWPFFGLVLPEKLPRLVGIQRVSR
                     RRMAAPAPGSSKVHEVEQAALIDTALTD"
     gene            complement(8536..9318)
                     /locus_tag="VFE39_01720"
     CDS             complement(8536..9318)
                     /locus_tag="VFE39_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257345.1"
                     /translation="MLDLAIAVSDRPMAPDQYRYVETHAWWLGTFGPHQHITEHRLRQ
                     WVPARPEREWVLDRELTGAQTWLTGSAHEAADDGFDLRDVAPVGRFRAQYGEFRTDGS
                     AESDHLCGPVTPRRGSWQSPTPQFIQELPLDSAALLARLREDNPGSWFGPFAAAVTTL
                     RTALVPAGLRAAFYCALTGLEGVDVDERVPNVDGHGCLAIIHDAGRTRTELLIDPADG
                     QFAGERDTLRSASRCGLPEGTVISTTAVRTAVVDAPGELPRS"
     gene            complement(9400..10431)
                     /locus_tag="VFE39_01725"
     CDS             complement(9400..10431)
                     /locus_tag="VFE39_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET6257346.1"
                     /translation="MLLSTQLQYSDDPIRNADAVVAMEQAGLDVVWVAEAYSFDAVSM
                     MGYLAAKTERVQIGSGILPIYSRTPTLTAMTAAGLDALSGGRAILGLGASGPQVIEGF
                     HGVPYDKPVARTREIIEICRKVWRRERLTNEGLYPIPLPEGQGTGLGKPLKLINHPQR
                     PDIPIWVASLGDKNVEMTAELADGWLPTVLMPEKLREVFGPALDAGTAKRAPELGPLQ
                     ITGGGILALEEEMFEPARQLARSMHALYIGGMGARGRNFYNTVFRRQGYADEAKLVQD
                     LYLDGKKEEAAAALPDDFIEKVTLVGTPSHVQERIAALRAAGVTHLHVSPVGTDPLKL
                     LAQVKEWIA"
     gene            complement(10529..11245)
                     /locus_tag="VFE39_01730"
     CDS             complement(10529..11245)
                     /locus_tag="VFE39_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6257347.1"
                     /translation="MVSSHYEKITAADGGVFDAFCAVPEAGPAPGVLIFQEIFGINDN
                     IRGLAERLAAAGYLALAPDMFWRIEPRFERKDESGMADGMERGGQLDLDLAGADITST
                     FAHLRAMPDCNGRVGGVGFCLGGTLAYLFAATSRVDGHGPTAVVSYYGSGIKGMLGLA
                     ERIECPILFHYGDRDPFITGEDIDAVERAVAGRQDATVLHYDAGHAFSNWDAPSLYDE
                     TSARVAWDRTIEFFDEHLRS"
     gene            complement(11348..12787)
                     /locus_tag="VFE39_01735"
                     /pseudo
     CDS             complement(11348..12787)
                     /locus_tag="VFE39_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_039638715.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            12935..>13365
                     /locus_tag="VFE39_01740"
     CDS             12935..>13365
                     /locus_tag="VFE39_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6257348.1"
                     /translation="MRFRVVPAAYVLPLRRVGRTDEVLLQLRCGTGYMDGHWAAAAAG
                     HVEADESVHAAACREAEEELGIGLVPADLVPLTVMHRTHGNHRAIDERVDFFFSCRTW
                     TGEPRRVEQAKSADLRWCSLDDLPEPVVPHELFVLAGLRRG"
BASE COUNT         1753 a         4544 c         5018 g         2050 t
ORIGIN      
        1 atcctggatc aacaaccagg ctcggacaca aggagggtgc accagtgagc gaggctgacg
       61 gcgacggcgt ggcgggggag caccgggcgc cctaccgggc ggtcggtggg cacgaggccg
      121 ccggtgagca caaggccggt cgtggctaca aggccggtcg tgagtacaag gaggtgctcg
      181 ccggcatcac tgcggccgtc gacgcgctgc gcgagcgcga ccgcgtccgg gccgcagggc
      241 acgcgaggga gctcgtgtcg ctcgaggacg acatgctgcg ggccggtgag cgcgccgcgc
      301 tgacccggct gggcgtcgag ctgcattggg aagcggcgct cgaggcgctc tggatggagt
      361 cgtggatgaa gctgcgaccc cgtcccgacc ccgacccccg cgccgggtcc ggcgctgggt
      421 ccgccgacct cgccgcactg gacgccgagg tcgagcagcg ctccgccgag ctgcaggacg
      481 cggtgcgccg caggcggttc gggttcccgc gccgctgacg cggtagcgcc gagatcgacg
      541 agggagtggc cggaccacgc tcgtggccat gctcatgtcg gactcgacgc cgaccagccg
      601 gtatgcggct cagctcgcga cgaccacctg cacctcgccg tcgtcccggg gcagcacgag
      661 cacgagtcgg tggccgcccg cggaggcgat gccggcggcc gtcgatgtgt cgaccgtgcg
      721 ccgggaccgg acgtcgagag ctgcccggtc gcctcctgca ttcgtgacct ggatggtgga
      781 gcctgtcgcg agtcccacgg tgccgacggt ctggggcagg gtgacgacgg tggtgcggcc
      841 ggcgtgtgcc agcgtcgcgg tgccgggtgt ggtcggtggg ccgggtagcc cgtcccctcc
      901 catcttcgag gagaccacct gcaacacctc gccgtccggc agcgtgatgc ttgtgggagt
      961 cggggcggcg tccggctggg gcaccgaggg gcgggagccg gccccctttg gcgcgctcgc
     1021 ccgcgtgctg ggcggtggcg ccgacgcgct gctcgccgga gcgctgcggg tgggggcgct
     1081 ggtggtgggc gcggtgcggg ccggtgcgct gctggtggag gcgttgtcgc ctggggcgcc
     1141 ggccgctggc gcgctcgcgg ctggtgcgct gccggtcggt gcgctgtcgt ggtcggtggg
     1201 tgcgctgtcg gctggtgggc tgttggccgg tgcattgccg gatggggtgg tgtcgggtgg
     1261 tgcgctgccg gttggggcgg taccggtttg agcgctgccg gtggggtcgg ctggggtgtt
     1321 gctggtgggg gcgctgttgg tggccgcgtc tgcggaactg atcggggtgc tgctcgtcgg
     1381 ggtgctgccg gatcgggcgc tgctcgtcgg ggtgctgctg ggtgcggcgg cggtgagtgt
     1441 ggggtcgtcc gtcggggtgg cagcggtcat ggtgccggtg gctgtgctgc caggtgccgt
     1501 gctgccgggt gtctcgctgc ccccggcgag gctgggcggg gcggcctgct cgccgccgcg
     1561 gacgggggca ggggcgagct gaggtgcgat ctgtgggacg tccggtgccg gtggcgcgcg
     1621 gaactcggtg ggcgcgcggg gcgcatcggg agcgggtacc ggggggacct ccgccggcag
     1681 gtccggcttg gccagcaccc cgggcgcgtc gcgacctgcg gcgggacggg tggcgcccag
     1741 gtagtcgtcg ccgggatagc ggacgtaggc cgtgcggacc ggctccccga acgtcggggc
     1801 gttgatcatg cgacctcccc cgatgtacag gccgacgtgg tgaacggcct cgggtacccc
     1861 gtagaagacg aggtcgccgg gctgtagcgg ggccccggcc ggcaggtgcg gccccctgta
     1921 gtactgcgtg tgagcggtgc ggggcaggga gatgccggcg tagtcgtagg acgccgtggt
     1981 caggccggag cagtcgaagc cggcctcacc tgactgcggg ccgtcaccgc cccacacgta
     2041 gggcaggccg cgctgggcga tcgcgaaggc gatcgccgcc gcggccgcgg tgccgggctt
     2101 cgcggtggtg gtgatcctcg gcagtgccgg cgtgcgcacg gcggggagcg gcgcggccgc
     2161 tgcgggcggg gcaggtccgg ccgagcgtgc tggcaggtcc gcctcggtca ggccgagcac
     2221 gagcgggccg gtgcggccga agttgacccc ggcgagtttc ggccggcggg agacggtgct
     2281 cgccggcagg ggcgcggatt ccggtgaccg cgagacctcc gtcctcgggt ccgcgggcgc
     2341 gctcgggacc tggggctccg tcgggctgac gggctccatc gcgaccgggt gccccggcag
     2401 ggcgggcgct gtcatcccga cagggggtgc caccgcggag ctgggcatga ccagccccag
     2461 ggcggccata cccatgacga ggatcacgac gaaaggccac cggcgcgagc ggtagcgctc
     2521 ccgcatctcg cctcccgctc cgagggctgc ctgctcacac cccccgatcg tgcccccggc
     2581 tgggggtgcg cgccagatag agcgggttcc gtcgcacact ctcagctgcg cgtcagccgc
     2641 gacacgagac ggttaacggg cgcacgatgg tggcatgatc ttcaccgacg cgcgcgaact
     2701 ggcccggatg ctgagggcga aggagatctc cgcccgggag gtgctcgacg cgcacctggc
     2761 gcagatcgag cgctgcaacc cggacgtgaa cgccatcgtg acgctcgtcg ccgaccgcgc
     2821 agcggacgag gcggcggccg ccgacgagcg actggcccac ggcgacgagg tcgggccgct
     2881 gcacgggttg cccatcgcgc acaaggacac gcacgacacc gccgggatcc gcaccacgta
     2941 cggctcacca ctgctggcgg acaatgtgcc gacgcgggac gcgctggtcg tggaacggat
     3001 acgggcggcg ggtgcgatca cgatcggcaa gaccaacgtg ccggagttcg ccgccggcag
     3061 ccacaccatc aaccccgtct tcggaccgac gcgcaacccc tacgccccca gccggtcgcc
     3121 cggcggaagc agtggtggcg ccgcggccgc gctcgcctgc ggcatgcacc cgctcgccga
     3181 cggcagcgac atgggcgggt cgctgcgcaa ccccgcctcg tactgcaacg tggtcgggct
     3241 gcgcccctcg ccgggccggg tccccgcgtg gccggatgcg gcgccgtggt cgaccctcgc
     3301 cgtgcccggc ccgatggccc gcacggtctc cgacgtcgcg ctcctgctcg gcgtgctgac
     3361 cggacccgac ccgcgcgacc ccacggcgct cgacaccccc ggggcggtgt tcgccgccga
     3421 gccgcccacc gacgtccgcg gcctgcgcgt cgccgtggca cctgacctcg gcggagccgt
     3481 gccggtggac ccggccgtca aagtcgtggt cgagcgggct gcgcgcgtgc tggaggaact
     3541 gggctgcgtg gtcgagcccg cctgtccgga cctgaccggg gcggacgagg cgttccggac
     3601 actgcgggcg tggcagttcg agctcgcgta ctccgcgatg ctcgacgcgc accctgacga
     3661 cttgaaggcg acgcttcgcg acaacatcct cgccggtcga actctcaccg gaccggacgt
     3721 cggccgtgcg catgtcctgc aggcggcgct gtaccaccgg gtgcgcgagt tcttcggccg
     3781 ttacgacgcg ctgctcgcgc cggtgagcca ggttccgccc ttcccgatcg agtgggagta
     3841 cccgaccgag gtggccggcg tgccgatgca cgactatctc gagtggatgg gctcggccta
     3901 cctgatctcg gcgacgggct gcccggcgat ctcggtgccg gccggcttca ccccggacgg
     3961 cctgccggtc ggcctccaga tcgtcggccg acaccggggc gagctcgggc tgttgcggat
     4021 cggcagggcg ttcgagcagg cgaccggcca cggccgaacc cgccccaggg gggcggcacc
     4081 aggtgacacg tcgaccagtt cttgatcatt ggtccggcgc cgcgagcggc gtgctgcaca
     4141 gctccgcgtt ccgaaatggt gatcttggtg cggtccccgg tggtgcggcc gcggtctgat
     4201 cggcggttcg gtaccgggaa cggcgcgcag cacgggtccc ggtacccgaa caatgatcgg
     4261 agttgccggc ggcggacggc gactcccacg aaccccggaa ccggccgcga acggccggtt
     4321 ccggggcagg acgggcgtcc agctactcgt cgaggtcgtc gctgtcgtcg cgggccagga
     4381 agctcgccag acgttccacg gcatcctcga acgacgggtt ctggtcgacg aaggtccgca
     4441 agcgatcggc cagccactcg acgctgacct gctcctcgcc ccgccgctcg gtgagctcct
     4501 cgatgccgcg gtcggtgaag tacacggcct gtcgccctag tcggtgaggg ccgtgtcgat
     4561 cagcgctgcc tgctccacct cgtgcacctt ggacgacccc ggggccgggg cggccatccg
     4621 gcgccgcgac acccgttgga tcccgacgag ccggggcagc ttctccggca gcacgagccc
     4681 gaagaacggc caggcgccct ggttggccgg ctcctcctgc acccagcgca cgtcctcggc
     4741 gttggggtag cgctccagca cggcggcgag ctgacggtcc ggcaccgggt agagctgctc
     4801 gatgcgcacg atcgcggtgt cgtcgatctc ccgcttgtcg cgcgcggccg ccagctcgta
     4861 gtagatcttg ccgctgcaca gcaacagctt gcgcacgccc gaggcgggac cgtcgctctc
     4921 ccggaagcgc gggtcgtcga tcacggagcg gaagcggccg ccggtgaagt ccgacaacgg
     4981 gctcaccacg gcccggttgc gcagcatcga cttcggcgtg aagacgatca gcgggcggcg
     5041 cacgccgtcc atggcgtgcc ggcgcagcag gtggaagtag ttggcgggct ccgacgggag
     5101 cgccaccgtc atcgatccct ccgcgcacag ctgcaggaac cgctcgatgc ggccggacga
     5161 gtggtccggg ccctggccct ccagcccgtg cggcaggagc agcgcgatgt cggcggtctg
     5221 gccccacttg gcctcgcccg acgagatgaa ctcgtcgatg atcgactgcg caccgttgac
     5281 gaagtcgccg aactgcgcct cccaggccac gatcgcctcg gggttggcga cggagtagcc
     5341 gtactcgaag ccgacggctg cgaactccga cagcgccgag tcgtacggga ggaagcgctc
     5401 ctggtcctgg gagaggttgc gcagcgggta gtactctgca ccggtcttgc ggtcgatcac
     5461 cacggagtgc cgctgcacga acgtgccccg tcgggtgtcc tgccccgaca gccgcacgag
     5521 cttgccgtcc atcgccaggg agcccagggc gatcagctcg gcgaacgccc agtcgacgtt
     5581 gccctcacgc gacatctggt agcgccgctg cagcaccggc ttgacccgct ggtgcacggt
     5641 gaagccctcg gggagctcga cgtgcgcatc accgacgcgg tgcacgacct ccaggggcac
     5701 cgaggtgtcg aggtcggtgg ggaccgactg ctcctgctcc acggaggggc tcggggccgc
     5761 cggagtcttc tccagctccc tgacctcgtt gaacacgtgc tcgagctggt tggagaagtc
     5821 gcgcagcgcg tgctcggcct cctccatgga gatgtcgccg cggccgatca gcgactcggt
     5881 gtagatcttg cggacgctgc gcttggcgtc gatcacgtcg tacatcgcag gctgcgtcat
     5941 cgacgggtcg tcgccctcgt tgtgaccgcg cctgcggtag cagatcatgt cgatcacgac
     6001 gtcgttgttc cagcgctcgc ggtactcgac ggccagcttg gccacccaga cgcaggcctc
     6061 cgggtcgtcg ccgttcacgt ggaagacggg cgcgtcgatc atcttcgcga cgtcggtgga
     6121 gtactgcgac gaccgcgcgt gctccggggc ggtggtgaac ccgacctggt tgttgaccac
     6181 cacgtgcacg gtgccgccgg tgcggtagcc gcgcagcttc gccaggttca gcgtctcggc
     6241 caccacgccc tgaccggcga acgccgcatc gccgtgcatc atcagcggca ggacgctgaa
     6301 gccgccctcg cccttgttga gcaggtcctg cttggcccgg acgatgccct ccagcaccgg
     6361 gtcgactgcc tccaggtgcg aggggttcga cgcgagggag acgacggtct cgccgtcgcc
     6421 gaacatccgg aagtacttcc cctcggcgcc caggtggtac ttgacgtcac cggagccgtg
     6481 ggcctggccg gggtcgagat tgccctcgaa ctcgcggaag atctggctga tcggcttgcc
     6541 gacgatgttg gccagcacgt tgagcctgcc gcggtgcggc atgccgatga ccacctcgtc
     6601 gagctcgtgc tcggctgcct tgtccagcac ggcgtccagc agcgggatga cggtctcgcc
     6661 gccttccagg gagaaccgct tctgcccgac gtacttggtc tgcaggaagg tctcgaacgc
     6721 ctcggcggcg ttgagcttgc tcagcacgta cttctgctcg gccgggttcg gcttctggtg
     6781 cggcacctcg atgcgctcct gcagccagtt gcgctgctcg gggtcggcga tgtgcatgta
     6841 ctccgtgccg atggtgcggc agtaggagtc gcgcagcagg ccgagcacgt cgcgcagctt
     6901 catccgctcc ttgccgccga agccgccgac ggcgaactcg cggtcgaggt cccacagcgt
     6961 gaggccgtgc gagagcacgt cgaggtctgg gtggcggcgc tggcggtagt tcagcgggtc
     7021 ggtgtcggcc atcaggtgcc cgcgtgtgcg gtaggcgtcg atcagctcga tgacgcgggc
     7081 ggtcttgtcg acggcgccgt ccgggatgtc ctgcacccag cggaccggct cgtacggcac
     7141 ccgcagcgag cggaagatgt cgtcgtagaa ggcgtcctcg ccgagcagca gcgcgtgcac
     7201 ccggcgcagg aagtcgcccg actccgcgcc ctggatgatg cgatggtcgt aggtggaggt
     7261 cagcgtgatg atcttgctga taccgatctc ggcgagccgc tcgtcgctgg cgccctggaa
     7321 ctccgccggg tactccatcg cgccgacgcc gatgatcgtg ccctggcccg tcatgagccg
     7381 cggcaccgag tggttggtgc ccagcgtgcc ggggttggtg aggctgatcg tggtgccggc
     7441 gaagtcctcg gactgcaggt tgccgcccct ggccttgcgc acgatgtcct cgtaagccga
     7501 ccagaactgg gcgaagttca tcgcctcgca gcccttgatc gagacgacga cgagcgagcg
     7561 ctgcccgttc ttgcccggca ggtcgatcgc gagcccgagg ttgacgtgct cgggctggac
     7621 gaccgtgggc ttgccgtcgg tctcgacgaa gtgccggttc atcaccggga agtcggcgag
     7681 cgcctgcacc acggcgaagc cgatgaggtg ggtgaaggag atcttgccgc cgcgggtgcg
     7741 ggtgagctgg ttgttgatca ccacgcggtt gtcggcgagc agcttcgccg gcaccgcgcg
     7801 gacgctcgtg gccgtgggta cggtgagcga ggcgttcatg ttcttgacca cggcaccggc
     7861 ggcgccgcgc agcggtgtgg ccctgccctc gctgctcacc gggctgggct tcgcggcctg
     7921 ggaggtggtg ccggcggccg gcttgtcgga ggtgccggtc ggcgcagcgg agctcgtggc
     7981 gggggtcttc tccgcggccg tgcggccacc ggtcgcggtg gccgtgccgt tggaggtggt
     8041 gccgttggag gtggtgccgt tggaggtggt gcggttcggg gaggcggtgc ccgtgcggga
     8101 ggaggcggtg gggggcggtg tcatcgcctt gcggtcgccg acacggcgac gctcggtgga
     8161 ctcggcaggc tcggcggtgg gggtgggcgc cggcgacgtg ggggcggacg atcgcggggc
     8221 ggtgcgggtg gccacaccgt tgccggtggg agcactggtg tcgtcggcag gacggtagtc
     8281 ggcgaagaac tcgtgccacg ccgggtcgac cgactcaggg tcgtcgagaa agcgctggta
     8341 catctcctcg acgagccatt cgttggggcc gaactggcct gctggattcg aggtactgct
     8401 gctggacacg ctgaagaccg ccttgatcgg atcgcttcgt catcgccgag aactgcaccc
     8461 caggttagtc ctcccggatc aggagcgggt accgcgcgct gggccgggcg tagcatgtcc
     8521 tgccgctacc cgtgctcagc tgcgcggaag ctcgcccggg gcgtcgacca cggccgtgcg
     8581 taccgccgtg gtgctgatca cggtgccttc gggcagtccg cagcgggagg ccgagcgcag
     8641 ggtgtcgcgc tcgcccgcga actggccgtc ggccgggtcg atcagcagtt cggtgcgggt
     8701 gcggccagcg tcgtggatga tggcgaggca cccgtgcccg tcgacgttcg gcacccgctc
     8761 gtccacgtcc acgccctcca ggccggtcag tgcgcagtag aacgccgctc gcagaccggc
     8821 cgggaccagc gccgtgcgca gcgttgtgac agccgcggcg aacggcccga accagctccc
     8881 cgggttgtcc tcccgcaggc gcgcgagcag cgcggccgag tcgagtggaa gctcctggat
     8941 gaactgcggt gtcggcgact gccagctgcc ccggcgtggt gtgaccgggc cgcagaggtg
     9001 gtcggactcc gcgctgccgt cggtccggaa ctcgccgtac tgcgcccgga accggccgac
     9061 gggcgccacg tcccgcaggt cgaagccgtc gtccgccgcc tcgtgcgccg agcccgtcaa
     9121 ccaggtctgg gcgcccgtca actcccgatc cagcacccac tcgcgctcgg gacgggcggg
     9181 cacccactgc cggagccggt gctcggtgat gtgctggtgc ggaccgaagg tccccagcca
     9241 ccaggcgtgc gtctcgacgt accggtactg gtcgggtgcc atcggtcggt cggacacggc
     9301 gatggcaagg tcgagcaacg cacgctcctg cggtcgtgag gctggtcacc tcagagtgtg
     9361 gcttgatgac cctccgtcac tcacagggcc ccccctcgct caggcgatcc attccttgac
     9421 ctgggcgaga agcttcagcg ggtcggtgcc gaccgggctc acgtgcagat gggtcacgcc
     9481 ggccgcgcgc agcgccgcga tgcgctcctg tacgtgcgac ggggtgccga cgagcgtgac
     9541 cttctcgatg aagtcgtccg gcagcgccgc ggcggcctcc tccttcttgc cgtcgaggta
     9601 gaggtcctgc acgagcttcg cctcgtcggc gtaaccctgg cggcggaaga cggtgttgta
     9661 gaagttgcgg ccgcgcgcgc ccatcccgcc gatgtagagg gcgtgcatgc tgcgggcgag
     9721 ctgccgcgcg ggctcgaaca tctcctcctc gagcgcgagg atcccgccgc cggtgatctg
     9781 cagcggaccg agctcggggg cgcgcttggc ggtgcccgcg tccagtgccg gcccgaagac
     9841 ctcgcgcagc ttctccggca tcaggacggt cggcagccag ccgtccgcga gttctgccgt
     9901 catctcgacg ttcttgtcgc cgagcgatgc cacccagatg gggatgtcgg gccgctgcgg
     9961 gtggttgatc agcttgagcg gcttgcccag tccggtgccc tggccctcgg gcaacgggat
    10021 cgggtaaagg ccctcgttcg tcagccgctc gcgacgccac accttccggc agatctcgat
    10081 gatctcgcgg gtgcgggcga ccggcttgtc gtaggggacg ccgtggaacc cctcgatgac
    10141 ctgcgggccg gacgcaccga ggccgaggat ggcccgaccg ccggagagcg cgtcgagacc
    10201 ggccgccgtc atcgccgtca gggtgggggt gcggctgtag atcggcagga tgcccgagcc
    10261 gatctgcacg cgctcggtct tcgccgccag gtatcccatc atggacaccg cgtcgaagct
    10321 gtaggcctcg gccacccaca cgacgtccag gcccgcttgc tccatggcga ccaccgcatc
    10381 cgcgttccgg atcgggtcgt cgctgtactg gagttgtgtc gagagcagca tcgggctagc
    10441 caacacaggg gttactggtg agtcaagcac gctgattcga tcgctcctgc caagggattc
    10501 gagcccgtcc gaatcgggcc gtccctgatc agctgcgcag gtgctcgtcg aagaactcga
    10561 tcgtccggtc ccaggcgacc cgcgccgagg tctcgtcgta caacgagggg gcgtcccagt
    10621 tggagaaggc gtgcccggcg tcgtagtgga gcacggtggc gtcctgtcgc ccggccacgg
    10681 cccgctcgac ggcgtcgatg tcctccccgg tgatgaacgg gtcgcggtcg ccgtagtgga
    10741 agaggatcgg gcactcgatc cgctccgcca gcccgagcat gcccttgatc ccggagccgt
    10801 agtaggagac cacggcggtc ggcccgtggc cgtccacgcg ggaggtggcg gcgaagaggt
    10861 aggcgagcgt gccgccgagg cagaacccca cgccgcccac ccggccgttg cagtcgggca
    10921 tcgcgcgcag gtgcgcgaag gtggaggtga tgtcggcacc cgcgaggtcg aggtcgagct
    10981 gcccgccgcg ctccatgccg tcggccatgc cggactcgtc cttgcgctcg aaccggggct
    11041 cgatccgcca gaacatgtcg ggggccagcg ccaggtatcc ggcggcggcg agccgctccg
    11101 ccaggccgcg aatgttgtcg ttgatgccga agatctcctg gaagatcagc acgccgggcg
    11161 cgggcccggc ctccgggacc gcgcagaacg cgtcgaagac acccccgtcc gcggcggtga
    11221 tcttctcgta gtgggagctg accatggtgc gcagcctagc tacttcgacc tgaagaaggc
    11281 cgatcgctcg aatcaggcgg ctggtagtcg acccggacca gggtgttgat ctccgttcgg
    11341 gggtcgatca cgcagcgagg gtcgagatct tgacgaggtt gtgggccagg accccgtgtc
    11401 cggcccagat ctgggcccct tccaggtcat ccaggcgcgt gcggtcccac ccgtagcctc
    11461 gtttgagggt gctgatgcgg gcttcgctgc cggtgcgcca cttgacggtt ctgcggaacg
    11521 cgcggcggtg ttcgtgggct tggcgggcct tgccgggtct gcccttgcgg gggatgacca
    11581 cggtgcgcac cccgaggtcg tgcagctcgt tctcgacctt ggcttcgccg tagccgcggt
    11641 cggcggtgac ggtgcgcggc cgccgcccgg tgcggcggat gacccggccg accgcgggag
    11701 cgagttgcgg cgcatcgggc gggttgccgc gttcgacggt gtggtcgacg acgatgccgt
    11761 cgacgttgtc gaggacctgg gccttgtagc cgaactcgac cggccggccg agccggcctt
    11821 tcgcgatcgg gcgggcatcg gcgtcgtgca ggctcacccg gcgggtcgcc ccgtccgggg
    11881 tggtgccaga caggcgttgg cgggtctgcg cggtgatccg gcgggtcgcg tccagcaggg
    11941 cggcgaggtc gttcacggcc cggcggagcc ggccgcggcg gcgcccggcg gccgggtcac
    12001 gagcatcggc ggcggcgagc tgggcggcct tggcgtcggc gcgtcgcagc gcccggcggg
    12061 cattgaccag caactgctcg gcctcagcgg cggccttccc ggccagccct gccagctcgc
    12121 cggtgatccg gcgaaccacg gcctgggctt cgtcgcgggt ctacgcgcta cgcagccgca
    12181 gcttcgcccc gatctcccgg gcccgccgac cggccgagcg gctccgatca cggaccttgg
    12241 tgcgggtggc tccgcccgcg gcctgcaccc gacgcccggc cgcggcgatc cgccgaaccg
    12301 ccttggccag caacccggag tcggtcggat aggccacatc ggccgggacc acggtggtgt
    12361 ccgcgcgcag ccgcgaggtg cgcagcagct tcgcctcggc ggccttggcc agcagcgcct
    12421 cgttgcagcc ctcgacagcg gccgtcccgc agcgggtggt gagcttcatc agcgtggtcg
    12481 agtgcggcac ccgcccgtcg atcgggatcc ggcaaaaccg ccgccaggtg atcgagtcgg
    12541 cgacctctcg gcacagcgac tcgtagccga gcttgtagcg gaacttgagg aacatcagcc
    12601 gcaggtagac ctccatcggc gtggacggcc ggcccagccg cgggtcgaac aacgatacga
    12661 acggggcgaa gaacgccgga tcgtccagca gcgcgtcgac ccgggccagc tcctcgggca
    12721 gccgcagcac ctcgtcgggc agcaccgcct cccacagact cacctcgagt gcgcgcgtcc
    12781 gcagcatcag gccccgtttc cccagctcac caccctgccg agccattctc ccgttcatcc
    12841 gacggtgatc gcgggaggcg cgccgacaca tcagcgcagg tcagaagccc gacacgcccc
    12901 tacttcttca ggtcgaagta gctaggctgc cgacgtgagg ttccgcgtcg tcccggccgc
    12961 gtacgtcctc cccctccggc gcgtgggacg gaccgacgag gtgctcctgc agctgcgatg
    13021 cggcacgggc tacatggacg ggcactgggc ggcggccgcg gcgggtcacg tcgaggccga
    13081 cgagtcggtg cacgcagccg cgtgccgcga agccgaggag gagctgggca tcggcctcgt
    13141 ccccgccgac ctcgtgccgc tcaccgtgat gcaccgcacg cacggcaacc accgcgcgat
    13201 cgacgaacgg gtcgacttct tcttctcatg ccgcacctgg acgggggagc ctcggcgcgt
    13261 ggagcaggcg aagtcggcgg atctgcgctg gtgcagcctc gacgacctgc cggagccggt
    13321 ggtgccgcac gagcttttcg tgctggcggg gctgcggaga gggga
//
LOCUS       DASRSC010000037         8949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1813425,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000037 DASRSC010000000
VERSION     DASRSC010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8949
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1813425"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFE39_01745"
     CDS             complement(<1..314)
                     /locus_tag="VFE39_01745"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET6257349.1"
                     /translation="MPEQAGSAVGFVGLGRMGDPMVRRLAAAGVVVRAHDVAAAPRKA
                     LADVVTVVDKPAEVVPDVGGDLSVVVLMLPDSKVVEAVLTDGLLDALVPGTLLVDMGS
                     SEP"
     gene            complement(307..708)
                     /gene="pcaC"
                     /locus_tag="VFE39_01750"
     CDS             complement(307..708)
                     /gene="pcaC"
                     /locus_tag="VFE39_01750"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HET6257350.1"
                     /translation="MTDDRYQRGLAIRREVLGEAHVDRSLAGVSDFSRPVQEFVTSAA
                     WGEIWSRPGLDRRTRSLLNLVMLTALNRNHELGVHVRGAVTNGCTETEIQEALLQAAV
                     YCGAPAALESFRVAERVLGELAAERTGVSHA"
     gene            complement(705..1115)
                     /locus_tag="VFE39_01755"
     CDS             complement(705..1115)
                     /locus_tag="VFE39_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6257351.1"
                     /translation="MQIVRGRAPDSTPTQARGDTFTGTVWGDPVLPTTPEGNTINSVT
                     FTPGARTYWHRHSRGQILVVTAGRGWVCTHGELPQVIRAGDVVWVPPDERHWHGGTVD
                     TVMTHLAVSLGPAVWLDEVDEDEYRAALAPEGER"
     gene            complement(1360..2199)
                     /locus_tag="VFE39_01760"
     CDS             complement(1360..2199)
                     /locus_tag="VFE39_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6257352.1"
                     /translation="MPVQSLSGRVAVVTGAAGGIGSAIASRLSRDGASVVLVDLDGDR
                     ASALAGRLGGEAIGVQADVSHEEDVERYMDAAVERFGRVDLHHLNAGIPGSAAALPDL
                     GAEEFDRLIAMNLRGTFLGLRAAFRQYFLQGSGGAIVVTGSIATLRGRSDLLPDHASK
                     HGVLGLMRNAAAYGGPLGVRVNAIAPGLVPTALFGPGRGPGGGGDMVKRASTTPMRKV
                     ANPEEIASVVAFLLSDDAAYTTGEVISVDGGASIVVNSVRPSGGAGAWDTTVQDARIA
                     ARS"
     gene            complement(2349..3077)
                     /locus_tag="VFE39_01765"
     CDS             complement(2349..3077)
                     /locus_tag="VFE39_01765"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6257353.1"
                     /translation="MSEASSGQVASLASQIGRVAAPLRSQVLEVLREAILSFRFAPGQ
                     RLIERELIDMTGVSRTTIREVLRELAAEGLVKTIPQKGAVVVSLTPEEAEELYEIREV
                     LEGHLAKRFVERATDAQVVALRRAFTQLEGVVERQEGTVAVLRAKDVLYEVLLTGAGN
                     SALRATLSQLHARVSLLRASSLASGPDRPSEAVGELRELVEAVEARDARDAADAMVAH
                     VGAAAGAGIEAVRSSGHALATAAN"
     gene            complement(3481..5007)
                     /locus_tag="VFE39_01770"
     CDS             complement(3481..5007)
                     /locus_tag="VFE39_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005152768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET6257354.1"
                     /translation="MTNAVPTIAKGIGLRSERGPVLIAVMLSTGLVALDSTIIATAVP
                     SIVSNLGGFEQFPWLFSIYLLTQAVTVPLYGKFADVLGRKPVMFFGIGVFLLGSVLCG
                     AAWSMPVLIGARAIQGIGAGAVMPISLTMVGDMYTVEERARVQGYLASVWAIASVVGP
                     TLGGVFAEYLSWRWIFFVNLPLGAIAVLTLAAKFTERVVRRKHHIDYLGATLLTLGCS
                     LVILGLLEGGVAWQWGSVQSVLVFALSVVMLVGFVLVERRAAEPVLPLWVFSRRTLVG
                     GNLVSLVVGALLIGLSSYLPTFVEGVIGTGALVAGFTLAALTIGWPIAASVAGPIYMR
                     IGFRDTGLIGSVFIILGAVLISLLPIDAAVWEAAAASFVLGIGLGAAASPTLVAVQST
                     VGWDRRGVVTGTNMFSRSMGSAVGAAVFGAIANATLADRFAHPPAEVAGHLPPSADAT
                     SLVLGGHTDPTSPTAVFVRSALYDATHHVFLWMVGVAALSVVGLLLMPRRTTELTFSP
                     "
     gene            complement(5285..5848)
                     /locus_tag="VFE39_01775"
     CDS             complement(5285..5848)
                     /locus_tag="VFE39_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257355.1"
                     /translation="MTLDLMTARPRQAQPAEHRLPWRERMRERRRLAHQDRLAAHLAE
                     LHHIRSLVAGARTVVRSGWVQDSWFAYRDEQGQERAVTAQNVQRMEGRPVTGACLVGA
                     IVHAGGGLAALHSQPVQRALDLTWHTLYGDEREPVRWCPAPPVRTAHVRDLTRWNDDP
                     GRSPREVTALLHTVDRAAAAEIERVRA"
     gene            complement(6059..7438)
                     /locus_tag="VFE39_01780"
     CDS             complement(6059..7438)
                     /locus_tag="VFE39_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HET6257356.1"
                     /translation="MSTSTAPIYLAGHLAPVPDEIDARELPVTGALPAELTGRYLRNG
                     PNPMPGEDPGHWFTGHGMLHGIRLRDGRADWYRNRWVRTGALAGRPFIRLDGTLDRTA
                     VVANTHVIGHAGKILALVENGFPYEVTDELDTVGPCDFGGALTTAMTAHPKTDPTTGE
                     LHFFGYGFTPPYVTYHRLAADGQLVETVPIEVPGPTMMHDFAVTEHHAVFLDLPMTFQ
                     LRKGMPFDWSDEYGARLGVMRLDRPGEVRWFEIDPCYVFHVGNAHEDAAGRIVLDVAR
                     YARADIVALWGPLGGAEATDPAGPAATAAASGLARLHRFTLDPVSGAVTETPLDERGI
                     EFPTVDDDRAGRAGRYLYAVGDAGLASGDNAIIRFDAQHGTTSAHELGPDHVAGEAVF
                     VPAAAPGRAEDEGWLLSIVTRRDGSASELLVLDATDVAGAPVATVTLPRGVPSGFHGS
                     WIPDAAPER"
     gene            7532..8086
                     /locus_tag="VFE39_01785"
     CDS             7532..8086
                     /locus_tag="VFE39_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET6257357.1"
                     /translation="MSLPHALLGLLAVEPASGYALTKAFETDLGRYAWHAGHTSIYPE
                     LGRLAEQGLVEVTAEGPRGSRTYAVTPAGRAELRSWLLDDPAGHSRRVRNEQVLRMFL
                     LSALDPADARIALARIAENTAAGVAELRRVRTAHADKPLIGREGFGQLAAEYGLRQYQ
                     AAHDWAIWAMTQLDENTESVGLER"
     gene            complement(8349..8738)
                     /locus_tag="VFE39_01790"
     CDS             complement(8349..8738)
                     /locus_tag="VFE39_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257358.1"
                     /translation="MEIAAVVILLVTGLANGLPIVGVVGARRLAGLYGVPITDPGLLV
                     LMRHRAVLLGLLGAALVGAAFVPSWRLPAMVAGLASMLAFVALASTTSGPKIRTTAVV
                     DVVLSVALAVALVLHLLADGTAATTTR"
BASE COUNT         1393 a         3190 c         3160 g         1206 t
ORIGIN      
        1 ggctccgagg agcccatgtc caccagcagc gtgcccggga cgagcgcgtc gagcagtccg
       61 tcggtcagca cggcctcgac caccttcgag tccggcagca tcagcaccac gaccgacagg
      121 tccccgccga cgtccggaac gacctcggcc ggcttgtcga cgaccgtcac gacgtccgcg
      181 agcgccttcc gcggcgcggc ggccacgtcg tgggcgcgca ccaccacccc ggctgcggcg
      241 agccgccgga ccatcgggtc tcccatccgc cccagcccga cgaacccgac ggcactccca
      301 gcctgctcag gcatggctca ccccggtgcg ctccgcggcg agctcgccga gcacccgctc
      361 ggccacccgg aacgactcga gcgccgcggg cgcaccgcaa tagaccgcgg cctgcaggag
      421 cgcctcctgg atctccgtct cggtgcatcc gttggtcacg gcgccccgca cgtgcacacc
      481 cagctcgtgg ttgcggttca gcgccgtgag catcacgagg ttgaggaggc tgcgggtgcg
      541 ccggtcgagg cccggccgac tccagatctc gccccaggcc gcactggtga cgaactcctg
      601 caccggacgg gagaagtcgc tcaccccagc cagcgaccgg tcgacgtggg cctcgcccag
      661 gacctcccgg cggatcgcca gtcctcgctg gtaccgatcg tcggtcatcg ttcaccctcc
      721 ggcgccagcg cggcgcggta ctcgtcctcg tcgacctcgt ccagccagac ggccgggccc
      781 agggacacgg cgaggtgcgt catcacggtg tcgaccgttc cgccgtgcca gtgccgctcg
      841 tccggcggca cccacaccac gtcgcctgcc cggatcacct gtggcaattc accatgcgtg
      901 cacacccagc cccggccggc ggtcaccaca aggatctggc cccgcgagtg gcggtgccag
      961 taggtgcgcg cgccgggggt gaaggtcacc gagttgatcg tgttgccctc aggggtggtg
     1021 ggcagcaccg ggtcgcccca caccgtgccg gtgaacgtgt caccgcgggc ctgcgtgggg
     1081 gtggagtcgg gggcgcggcc ccggactatc tgcatcgttc atcccttcgt tgtgtccgtc
     1141 gacgctggtg ccccccgcac atcaccgatc gcgtccagta cgcggttgcc catctggtcg
     1201 ccataaccga agttctctcc acccccaagc tcgcgagcgg gccccgcggc attgaggagc
     1261 ttgcctgcgc ctgccccggc agcaccccgg cttcgtcgcg cacgaccgag tcgacgaaca
     1321 acattgcgat caacgatgct ggccggagcg ccgtctccgt caggagcgcg ccgcgatgcg
     1381 ggcgtcttgc acggtggtgt cccacgcccc cgctccgccg gagggacgga cgctgttgac
     1441 gacgatcgag gcgcccccgt cgaccgagat gacctcgccg gtcgtgtacg cggcatcgtc
     1501 gctcagcagg aaggcgacca ccgacgcgat ctcctcgggg tttgccacct tgcgcatcgg
     1561 ggtggtcgag gcgcgcttca ccatgtcacc ccctccaccc gggccgcgcc ctggtccgaa
     1621 gagcgcggtc ggcacgaggc cgggcgcgat ggcgttcacc cgcaccccga gcggtccgcc
     1681 ataggctgcc gcattgcgca tcaagccgag cacgccgtgc ttcgaggcgt ggtctgggag
     1741 gaggtccgac ctgccccgca gggtcgcgat ggaaccggtg acgacgatcg cgccaccgct
     1801 gccctgcagg aagtactggc ggaacgcggc ccgcaggccg aggaacgtcc cccgcaggtt
     1861 catcgcgatc aggcggtcga actcctcggc gccgaggtcc ggcagggcag cggcggagcc
     1921 ggggatcccc gcgttgaggt ggtgcaggtc gacgcggccg aaccgctcca ccgcggcgtc
     1981 catgtaccgc tcgacgtcct cctcgtggga cacgtccgcc tgcacgccga tcgcctcgcc
     2041 cccgagccgg ccggccagcg ccgaggcgcg atcgccgtcg aggtcgacga gcacgacgga
     2101 ggcaccgtcg cgcgacaacc gggatgcaat ggcgctaccg atcccgccgg cggcaccggt
     2161 gactaccgcg actcgacccg acagggactg gaccggcata ccgactcctc acttcgcaac
     2221 acgcttgccc gactgtatga caatcagaca ggctttgcca cactgggagc gggcctaggg
     2281 tttgccggcc taccagcggc cgtcgggcca ggtggatcgg ctctagcttg tcgaggagcc
     2341 ggcgaaggtc agttggcggc agtggcgagc gcgtggcccg agctgcgcac cgcttcgatg
     2401 cccgcgccgg cggcggcgcc gacgtgggcc accatggcgt cggctgcgtc gcgtgcgtcc
     2461 cgcgcctcga cggcctcgac cagctcgcgc aactcgccga ccgcctccga cggccggtca
     2521 gggccggacg ccagcgaact cgcacgcagc aggctcacgc gggcgtgcag ctgcgagagg
     2581 gtggcccgca gagcagagtt gcccgccccg gtgagcagca cctcgtacag cacatccttc
     2641 gccctgagca cggccaccgt gccctcctgc cgctcgacca cgccctcgag ttgcgtgaac
     2701 gcgcggcgta gagccaccac ctgtgcgtcc gtcgcgcgct ccacgaatct ctttgcgaga
     2761 tgcccctcga ggacctcgcg gatctcgtag agctcctcgg cctcctcggg cgtgagcgag
     2821 acgacgacgg cgcccttctg cggaatcgtc ttgaccagac cctcggccgc cagctcgcgc
     2881 agcacctcac ggatggtcgt gcgggagacc ccggtcatgt cgatcagctc gcgctcgatc
     2941 aggcgctgcc ccggcgcgaa gcgaaagctc aggatggctt cacgcagaac ctcgagcacc
     3001 tggctccgca acggcgctgc cacgcggccg atctgcgagg cgagactcgc tacctgtccg
     3061 ctactggctt cgctcaccgg cttagcgtac ggcatgatcg tcatacgagc agcggattct
     3121 gcgcgagaac gtatcggacg aactatcgcg gaccgggcac cgacctaccc tagcgccggc
     3181 gagatcgtga tggggatcgc gatgatcgag acgagccgcg ccgccgcacc ccgccgtagt
     3241 catgcccgga ccggggtgga gccggttgcg gcttgcgtcg gcgagcagca cctgcgcttc
     3301 ggcgtcgcag gcgaaaggtt cggccgtggc ctgtgggatc tcggcgcggg ccgccggggt
     3361 gagccggccg ggaggagttg cgccccacca acctccggaa ctcggcggcg aacaggaccg
     3421 ctgtgccacc gcgcctcgga gacgagagtg caaccccgag cacctcagcc gccaccgacc
     3481 tcagggagag aaggtgagct ccgtcgtccg gcggggcatc agcagcaggc ccaccacgct
     3541 cagggcggcg acgccgacca tccacagaaa gacgtggtgg gtggcgtcgt agagggcgct
     3601 gcggacgaac accgcggtgg gcgacgtcgg gtccgtgtgg ccgccgagga cgaggctggt
     3661 ggcgtcggcg ctcggcggga ggtgtcccgc gacctcggcg ggagggtgtg cgaatcggtc
     3721 ggcgagggtg gcgttggcga tggcgccgaa caccgcagcg cccacggcgc tgcccatcga
     3781 gcggctgaac atgttcgtgc cggtcacgac gcctcggcgg tcccagccga cggtcgactg
     3841 cacggccacg agcgtggggc tcgcggcggc accgagaccg atgccgagca cgaaggacgc
     3901 ggcagcggcc tcccacacgg ccgcgtcgat cggcagcagg ctgatcagca cggctccgag
     3961 gatgatgaag acgctgccga tcagcccggt gtcccggaag ccgatccgca tgtagatggg
     4021 gccagcgacg gacgccgcga tgggccatcc gatggtcagt gccgcgagcg tgaagcccgc
     4081 cacgagcgcg ccagtaccga tgacgccttc gacgaaggtg ggcagatagg agctcaggcc
     4141 gatcagcagg gcgccgacga ccagggagac caggttcccg cctacgaggg tgcggcgact
     4201 gaacacccac aacggcagca cgggctcggc cgcccggcgc tcgacgagga cgaaaccgac
     4261 cagcatcacc acactcaggg cgaagaccag cacgctctgc accgagcccc actgccaggc
     4321 cacaccgcct tcgagcaggc cgaggatcac cagtgagcag ccgagggtga gcagtgtggc
     4381 gcccaggtag tcgatgtggt gcttgcgccg cacgacgcgt tccgtgaact tcgcggccag
     4441 cgtcagtacc gcgatcgcgc cgagcgggag gttcacgaag aagatccagc gccacgacag
     4501 gtactccgcg aagaccccgc cgagggtcgg gccgacgacc gacgcgatcg cccacacgct
     4561 ggcgagataa ccctgcacgc gggcgcgttc ctcgaccgtg tacatgtcac cgaccatcgt
     4621 caggctgatc ggcatcacag ctccggcacc gatgccctgg atcgcccgcg ccccgatgag
     4681 caccggcatg ctccacgccg cgccgcagag caccgaaccg agcaggaaca ccccgatgcc
     4741 gaagaacatc accggcttgc ggcccagtac gtcagcgaac ttgccgtaca gcggcacggt
     4801 caccgcctgc gtgagcaggt agatcgagaa cagccagggg aactgctcga acccgccgag
     4861 gttgctgaca atcgacggca ccgcagtggc gatgatcgtg ctgtcgagag ccaccagccc
     4921 ggtactcagc atgaccgcga tgagaactgg cccgcgttcc gagcggaggc cgattccctt
     4981 cgcaatggtc gggaccgcgt tggtcacggt ggggcactcc ttgagagctg cgggggcatc
     5041 gcgactactt cgcaatgcta gatattttac gctgctccgc cccgtgcccc ggtgatacag
     5101 cgggcgtgat ggtcgaaaga actctcacat ctcgctgccc agcggtcccc cggccgagcg
     5161 tcgcgcgcag cccgcgtgct cgaccgcctc gtgccgccac gacgagtcgg ctggtgcccg
     5221 caccgacgcg aggatcgcaa cgcccgccat accggccgcg cccatcgagc gcacaccgcg
     5281 tcgatcaggc gcgaactcgt tcgatctccg cggcagcggc ccggtcgacg gtgtgcagca
     5341 gtgccgtcac ctcccggggg ctgcggccgg ggtcgtcgtt ccagcgggtg aggtcacgga
     5401 cgtgggcggt gcggacgggc ggggccgggc accagcgcac gggctcgcgc tcgtcgccgt
     5461 agagcgtgtg ccaggtcagg tcgagcgccc gctgcacggg ttgggagtgc agtgcggcaa
     5521 gccccccgcc cgcgtgcacg atcgctccga ccaggcaggc gccggtcacc ggccggccct
     5581 ccatccgctg cacgttctgc gcggtgaccg cccgctcctg accctgctcg tcgcggtagg
     5641 cgaaccagct gtcctggacc caaccggacc ggaccacggt gcgtgcgccg gcaacgaggg
     5701 atcggatgtg gtggagctcc gccaggtgtg cggcgaggcg atcctggtgg gcgaggcgcc
     5761 gccgttcacg catccgctcc cgccacggga gccgatgctc cgcgggttgg gcttgccggg
     5821 gtcgtgcggt catgaggtcg agcgtcacaa cggtctcctt ccgacgggat caggccagta
     5881 aatatccagg tggcaaggtc ctcaaccgac agattcggca ccacccctcg gcagcgggag
     5941 cgatgagaga tcacgagcag gggctccaac ccgggcagcc cgcggtcgtg ctgggcctcg
     6001 gcacgatcag gctgcaggtc aggcaggtcc tgccggcgac ccggacggct gagtcggttc
     6061 agcgctccgg ggcggcgtcg gggatccacg atccgtggaa gcccgacggc acccctcggg
     6121 gcagggtgac ggtggcgacc ggcgccccgg ccacgtcggt ggcatcgagc acgagcagct
     6181 cggacgcgct gccgtcgcgg cgggtgacga tcgacagcag ccagccctcg tcctcggccc
     6241 ggccgggtgc ggcagcgggc acgaagaccg cctcgcccgc tacgtggtcg ggcccgagct
     6301 cgtgggccga cgtggtgcca tgctgcgcgt cgaagcggat gatcgcgttg tcgccgctcg
     6361 ccaggccggc gtcgcccacc gcgtacaggt agcgcccggc ccgtccggcg cggtcgtcgt
     6421 cgactgtggg gaactcgatg ccgcgctcgt cgagaggcgt ctcggtcacc gccccggaca
     6481 ccgggtcgag agtgaagcgg tgcagccgcg cgaggcctga ggcggcagca gtcgcggcgg
     6541 ggcctgccgg gtcggtggct tcggcaccac cgagcggtcc ccacagggcg acgatgtcgg
     6601 cgcgtgcgta gcgggcgacg tcgagcacga tccgcccggc cgcgtcctcg tgcgcgttgc
     6661 ccacgtggaa gacgtaacag gggtcgatct cgaaccagcg cacctcaccg ggacggtcca
     6721 gccgcatgac gccgagccgg gcgccgtact cgtcgctcca gtcgaagggc atgcccttgc
     6781 gcagctggaa cgtcatgggc aggtcgagga acaccgcgtg gtgctcggtg accgcgaagt
     6841 cgtgcatcat cgtcgggccc ggtacctcga tcgggacggt ctcgacgagc tgcccgtcgg
     6901 ctgcgagccg gtgataggtg acgtagggcg gcgtgaagcc gtagccgaag aagtgcagct
     6961 cgccggtggt cgggtcggtc ttcgggtgcg cggtcatcgc cgtggtgagc gcgcccccga
     7021 agtcgcaggg tccgacggtg tccagctcgt ccgtgacctc gtaggggaag ccgttctcga
     7081 ccagcgcgag gatcttcccg gcgtgcccga tcacgtgcgt gttggcgacg accgccgtgc
     7141 ggtcgagcgt gccgtcgagc cggatgaagg ggcgcccggc gagcgcgccg gtgcggaccc
     7201 aacggttgcg gtaccagtcc gcacgaccgt cgcgcagccg gatgccgtgc agcatgccgt
     7261 ggccggtgaa ccagtgcccc gggtcctcgc cgggcatcgg gttcgggccg ttccgcaggt
     7321 agcgacccgt cagctcggcc ggcagggcac cggtgaccgg cagctcgcgg gcgtcgatct
     7381 cgtcggggac cggagcgagg tgtccggcca ggtagatcgg agcggtgctg gtgctcatcg
     7441 ggaatccctt cgtcgcgaga acccgactgt ccccctcgtg gtcgaaacat gtcaatagtg
     7501 acaggtcgaa atcgtcatat aagatctcct catgtcgttg ccgcacgcgc tgctcgggtt
     7561 gctcgccgtc gaacccgcga gcggctatgc actcacgaag gcgttcgaga cggacctggg
     7621 ccgttacgcc tggcacgcgg gccacacgag catctacccc gagctcggcc ggctcgccga
     7681 gcagggactg gtcgaggtca cggccgaagg cccgcggggc agccggacct atgccgtcac
     7741 tcccgcgggg cgtgccgagc tgcggtcctg gctactggac gacccggcgg ggcacagccg
     7801 gagggtgcgc aacgagcagg tgctccggat gttcctgctg tcggcgctcg accccgccga
     7861 cgcacgcatc gcactcgcca ggatcgcgga gaacaccgcg gcaggcgtgg cggagctccg
     7921 ccgggtcagg acggcacatg ccgacaagcc cctcatcggc cgggaggggt tcgggcaact
     7981 ggccgcggag tacgggctgc gccagtacca ggccgcgcac gactgggcga tctgggccat
     8041 gacgcaactc gacgagaaca cggagtcggt cggcctggag cggtaggcca ggtcggtgtc
     8101 cgagccacgg ctggcactgt ccggctggtc acccgttcct ccgcctaacg atccccggcc
     8161 cggcggcccg gcgggcgccc gccggcgcgg ctggaccaag gacgaggagg tcccgtcggt
     8221 ggtcgagttc gagccggcgc cggatcaccg ccctctggtg acctacctcg gctccgcgcg
     8281 tgaatccgcg ccgtaggccg gcgccctccg cgggagcgag cgggaaatca aaccgtgaca
     8341 gcgtgtggtc agcgcgtcgt agtcgccgcc gtgccgtcgg cgagcaggtg gaggacaagt
     8401 gcgacggcga gcgctacgga gagcacgacg tccacgaccg ccgtggtcct gatcttcggg
     8461 cccgacgtgg tcgaggcgag agcgacgaac gcgagcatgc tcgccagtcc ggcgaccatg
     8521 gcggggagcc gccacgacgg gacgaacgcc gcgcccacga gagcggcacc gagcaacccg
     8581 agcagcaccg cgcggtggcg catgagcacg agcagaccgg gatcggtgat cgggacgccg
     8641 tacaggccgg cgagtcggcg ggccccgacg acgcccacga tggggagccc attggccagg
     8701 ccggtgacga gcaggatgac aaccgccgcg atctccacgc cgatcatcgt gggcgctcgt
     8761 ggcgcgaccg atcaatgatc tccgaggtca tggagagccg gtgggctcac gagccgagca
     8821 gctccgcccg cagcatcgcg gcggcgtcgg ccatggcccg catccagccc tccgcgccgg
     8881 cgcggggcag gcacctcccc gatcacgacg tgggcggcgt ccggtccgct ccgcccaccc
     8941 ggaggtccc
//
LOCUS       DASRSC010000038        24982 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3942345,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000038 DASRSC010000000
VERSION     DASRSC010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 24982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24982
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3942345"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..649
                     /locus_tag="VFE39_01795"
     CDS             26..649
                     /locus_tag="VFE39_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HET6257359.1"
                     /translation="MTRKRYEDLTRAGRNRVVTATLLRAVASAALLVLVYYFAPLDAA
                     LNADTVVWFVFALVLVGAVIAWQVRAILRSDVPRLRAIQAVAIGIPMLLLVFAAVYLL
                     IDTDRPGSFTEPLSRTDALYFTLTVFTTVGFGDITPKSELARIITMTQMVVGLIAVGL
                     VARILFSAVQIAVARRNGAGPLGPAAPDHDDARQPDPSPDTDAAGSG"
     gene            830..2239
                     /locus_tag="VFE39_01800"
     CDS             830..2239
                     /locus_tag="VFE39_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HET6257360.1"
                     /translation="MSQPTVDPTFYRSPAEAAAAPVEELAYVAAFDRGGTTNDAMTVV
                     DLNPDSDTYGKVVGWTDMPTVGDELHHFGWNACSSALKHEGHDMDGLARRYLLVPGLR
                     SSNIHVLDTGPDPRNPTHVKTIDRADLSAKAGYSRPHTLHCGPDGVFLTCLGGPEGND
                     DGPGGIALLDHETFDVVRAWETDRGPQHFHYDAWWHLNQNVLISSEWGSPSMIEDGLV
                     PELLLGQKYGHAIHFWDLATGRHQQRVDLGAQHQMALEVRPAHDPDATWGFVGVVVST
                     EDLSGSVWRWFRDGDEWKVEKVITIPAEPADPDVLPPALKPFGAVPPLVTDIDLSVDD
                     RFLYVSCWGIGEMKQFDVSDPAHPRQTGSVRLGGIVDRAPHPARPDVPIAGAPQMVEV
                     SRDGRRVYFTNSLYGAWDDQFYPDGVGAWMAKLDADPDGGLSIDENFFPHGEEFRGRR
                     VHQIRLQGGDASSDSYCYR"
     gene            complement(2271..2948)
                     /locus_tag="VFE39_01805"
     CDS             complement(2271..2948)
                     /locus_tag="VFE39_01805"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HET6257361.1"
                     /translation="MPQRARSVSVQLAAHFERLIATGVLRPGERLPPERELAATLSVS
                     RGSLREAMHELEAKNLVERRTGRGTIVAEQTESVTELYSELDGTDVTLDDATELRLIV
                     EPRIASAAAVRSGPSNLVQLEDVLTRSALHPDADMSLQLDVEFHLLLAHAAQNPLLVA
                     LCTMTSRWTETLRRHSHATPEGRLRSLEGHQAIYQAVLARDADAAAAAMNEHLSAIRD
                     VIARRTS"
     gene            3176..4093
                     /locus_tag="VFE39_01810"
     CDS             3176..4093
                     /locus_tag="VFE39_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6257362.1"
                     /translation="MLNRLLRPAALLASAGAITLLATACGGGSPATSGNAPAYPALAS
                     SPTCSQLRTQNPDLAGKTLTNAINPHTPGYESISVSDPTKYEGFDIDLGEAIGECLGF
                     KVSYLPVAFDAMLPTLQSGQANFIISDIYATKERAASADFITYSKVNDGVLVAAGNPK
                     KLTGIDDSLCGTTTALNKGFVEVPLVDAQSAKCQAAGKPPAQTALFDNNADCVQAILS
                     GRADSYINDVNTVARFVKDQPDKLARATAVTLPYSIGIAVPKGQLPIRNAFTAALTEI
                     QKSGLQTQLSKKWELDESAIEAPSLVTAS"
     gene            4102..5667
                     /locus_tag="VFE39_01815"
     CDS             4102..5667
                     /locus_tag="VFE39_01815"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease/ATP-binding
                     protein"
                     /protein_id="HET6257363.1"
                     /translation="MESFLHYLSMPYLLTGILITLQATALGFFGGFALGLLLAGMALS
                     RNRVVSGVARTYSVIFRGTPLVLQLVFVFTALPHAGVVLSPIMAAGVALAANEATFFA
                     EIFRSAVLGVDKGQTDAARAVGLRPGTVLRRIIGPQAARAAIPAIGSEAVSTMKNSAL
                     ASIIAVPELTLRSNQLASATFDYFSIFFASGVMYLILTGAITGLQLTIERALDLSRVG
                     RRGRGRPAVPSTVNGASVIETPLATKELTATRKVNGAVSRAEAVGRARVALTINDVHK
                     AFADHEVLRGIDLEIRKGEVVALLGPSGSGKSTLLRLINHLETLDTGKISFDGIEVGY
                     RDAGVPLPEREVARQRVDAGVGMVFQHFNLFGHLTARENVAVPLRWVHRVPKDEALAA
                     ADHLLGRVGLSARGHALPRQLSGGQQQRVGIARALAGRPQVLLLDEPTSALDPELVQE
                     VLEVVRSLAVDDGLTMLIATHQLGFARDVADRVVFMADGTVLEQGPPQQVLDNPKHPT
                     AVRFLRAVHTVEV"
     gene            5670..6092
                     /locus_tag="VFE39_01820"
     CDS             5670..6092
                     /locus_tag="VFE39_01820"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HET6257364.1"
                     /translation="MNPHRQSFSFTPEQGVPPAVAPFSHVTQWGSMLFVTGQMPTDPA
                     TGALVAGGLVPQAEQVRRNLLACLAPFGANLDHALMVRVYLTDFTEFEAFNALYRTWF
                     TTALPSRTCVGSTALAVGAEVEIDLIVGLPGHNIGASA"
     gene            6089..7168
                     /locus_tag="VFE39_01825"
     CDS             6089..7168
                     /locus_tag="VFE39_01825"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET6257365.1"
                     /translation="MNLTLLQPGEGNIPGRHLLASTPETVSWGWLPNEKSTPCLTVGS
                     GETVTIDTLSHEGILDDQGRDPVEFLGRYGVEAADVLHDAKAIAASTIPNEPDDGPHV
                     VTGPIEIAGAEPGDVLKIDVLELLPRTPYGFVSSRHGYGALPGELPEGAQSVFQFCRV
                     GVIDGRMVSWMAATDGRQACFPLDPFLGLMGVAPATSEPVHSVPPGDFGGNIDVKWAQ
                     VGTSLYLPVTVPGALFYTGDPHYAQGNGEVALTALEASLRATLRLTVLSGAEASTAMG
                     SLRNPLLETPGYWIPTGMDEDLREAMKKCVRHALTFLIERFGFDRSTAMAYLSAAGDF
                     EISQVVDVTKGVHCMIRKADFAQWY"
     gene            complement(7676..8296)
                     /locus_tag="VFE39_01830"
     CDS             complement(7676..8296)
                     /locus_tag="VFE39_01830"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET6257366.1"
                     /translation="MRRSRPAVVVFDVGGVLAEPEGGIAALAAEARVDVTGFTTAYWR
                     YRDGYDLGGAVDEYWTQVGADVGRTLTPAEVRRLDRGDAQRWAELAPRLVKLVVRVAH
                     SGVRMALFSNAPHSLAAEVRSSGWGALFPTKVFSCETGVAKPRSGAYEAVEDALGLSG
                     GELMFFDDRPANVAAARERGWSAHQWQGPWQCVADLAAGGVRILPG"
     gene            8545..8829
                     /locus_tag="VFE39_01835"
     CDS             8545..8829
                     /locus_tag="VFE39_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257367.1"
                     /translation="MATLTVWEFDTADGAQNALTTLERLQKEELIRVVDVAVVTWPAD
                     RKKPRTQDAVTDRVVENFRGTRAELISTNLSADQEAKLREAFEDADHAPV"
     gene            8990..9457
                     /locus_tag="VFE39_01840"
     CDS             8990..9457
                     /locus_tag="VFE39_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257368.1"
                     /translation="MSTSPQATPGNATGYGDAAGRAAAETSPWKTGFVVFAGTIMIVA
                     GVFQALAGLVALFRNEVYVVGLNYVFSFDVITWGWIHLIIGIVVAVAGAAVLTGQLWG
                     RVFGVGIAAVSMLTNFMFIPYYPVWSLLIIALDVFVIWALCTYTRDRSEAVTR"
     gene            9524..9697
                     /locus_tag="VFE39_01845"
     CDS             9524..9697
                     /locus_tag="VFE39_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257369.1"
                     /translation="MMIGILMVAFLPPLIIAVVLLLERVEESLGATGHDPRPASPNPG
                     AAGHLGSAGSRTD"
     gene            9781..10677
                     /locus_tag="VFE39_01850"
     CDS             9781..10677
                     /locus_tag="VFE39_01850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET6257370.1"
                     /translation="MRIGFGASVSGAWAGPENLAAFAERAEELGYDSLWTFQRLLTPA
                     DPEALDVVYRSVLDPMVALSYVAARTSRIRLGVAVVNLPFVSPAYLAKQASTLDVLSG
                     GRLDLGLGIGWQREEFAASGVTPERRGARAEEYLEALHTLFHDEVSAFDGEFYTLLPS
                     RMAPKPVGPAVLLGGAAEAALRRAGRLADGWMSRSATDLTKISGQIAVVREAAERAGK
                     DPAGVRIVVRGVVRAGGDSDQRLAGSYDKIREDTAWLGEQGVTEVFYDLNWDPQIGSP
                     DVDPAAATARAEEILHELSPGR"
     gene            complement(11276..11734)
                     /locus_tag="VFE39_01855"
     CDS             complement(11276..11734)
                     /locus_tag="VFE39_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6257371.1"
                     /translation="MDHTHLDRFGAQTWTAVMGHSSRGAFTITEEPDEPLDVRLHAPS
                     PDTVIVWVAGPLRRSTAPLLMLRVRQQLHRASHVILDLSSVGCLDAHAATELRTLRAQ
                     ADSCGTQLHIAGVSIDAIADLLRHVDPDQHLATGPADAVLASLAITPHAG"
     gene            complement(11742..12155)
                     /locus_tag="VFE39_01860"
     CDS             complement(11742..12155)
                     /locus_tag="VFE39_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6257372.1"
                     /translation="MTITSPLGRLTASASPPPAAPAIRSVQPDRDHGVLEVAGALCQD
                     NAAELRQRVEGLLVAGVRYLLVDLAEADGCDPAVMGVLADAARLLEGRQGWLRINPNG
                     TLPVAAKLDEATLPDLFAIYRASAGTSRPGGRHAR"
     gene            complement(13040..13726)
                     /locus_tag="VFE39_01865"
     CDS             complement(13040..13726)
                     /locus_tag="VFE39_01865"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET6257373.1"
                     /translation="MTIIDLSQDIYEGMKVYPGHLKTVQFEHVTHEETAPRFDSGFSF
                     QTTGFMLNDNGPTHVDSFSHLDPDPSAETIEKMSLDLFYGTAVCLDVSDFKPQTDITA
                     EDLDRVEAESPVDVRRGDIVLFHTATWNRYAGDKRYLSEFPGLGESASEWIVRRGVKT
                     FGVDSPTPDNPTSTSYPCHMMCRREHITHYENLANLDRVVNVRFTFVGFPLKLQGAHG
                     GPTRAVALVD"
     gene            complement(13752..14522)
                     /locus_tag="VFE39_01870"
     CDS             complement(13752..14522)
                     /locus_tag="VFE39_01870"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6257374.1"
                     /translation="MNARVAVVTGANRGIGRAIAVALATDGFDVAVTARDPATLADTL
                     AEVEQAGGTAVALPCDVREEGSVAAMAEAVAAIGPVHTVVANAGVAGPTAPLHEISLD
                     DWRDCVATDLDGVFLTFRAFVPSMINAREGSLIAISSMTGKRPLTGRTPYAASKMGVI
                     GLVRTLALELGPYGIRVNAVCPGAVAGPRIEDVVRKQAAARGISEDEALAVFTGSSPL
                     GRLVEADEVARTCSFLASAGAAAITGEDVNVTAGVVMY"
     gene            complement(14566..17001)
                     /locus_tag="VFE39_01875"
     CDS             complement(14566..17001)
                     /locus_tag="VFE39_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET6257375.1"
                     /translation="MTAVDRPSAPPVRTHAETRKWVGKSIRRVEDPKFLRGRGGYIAD
                     LVTPGTLHAAVLRSPHPHARIVSMDTAAAAALDGVHAVITGAQAAELTDPLPDFGPDP
                     ANHPWRCLAVDKVRYVGEGVAVAVADSRYLAEDALGLIDVEYEPLPPVVDPEAALADG
                     APLVHETLGTNCAYERTFDFGEVDRDFAEADVIVKDRLRWHRSGGQPLETVGAIADFD
                     YATGELTVHTNSLSFTSYLFMAAATLKIPANKLDVRQVPAGGSFGSKLFVTKPLVIAG
                     MCSRVVGQPVVFLEDRVDNMSNCDHHGSDRVYEVELAMMRDGTMRGLKIDTIDDYGAY
                     IQFGVGHHGNALAQVVGPYMIGSVRYRVRAVLTNKNQQGAYRGFGSEVNNWMLEQMVD
                     KAARELDLDPVEIRRRNFIREFPHFIPTGNVYDSGDYDRVLDKALALSEYDHWRAEQA
                     KAREEGRHIGIGLITAQERSVFSATEFWFWFDEPGAPVTSMPESVTLKVDATGGITAT
                     LYSCAFWGNSPETMVAQLVAEEFDCDPHDVAIVYQGSKNGLPATGPGGSRTTVMLAGA
                     VEGATAKIKAKALRAAAHLLEADPDDLEWVDGGYQIKGVPEQRKSLADIAVMLHLFKH
                     SFPDDMESGLEDSKVFDHPYTTMPSADRKDLGVFYPFMGHACHVPVVEVDTETGGVRI
                     LSYAAVHDCGTLVNPRSLAGHIVGGTAQGIGTAFYEEFVYDDDGQLLSSSYLDYLIPS
                     AMEVPELAIGHVETPSPYTPHGIKGGGEGGRMMAPAALNAAVNDALAPLGVRLTELPM
                     TPERILAALRAAR"
     gene            complement(16998..17534)
                     /locus_tag="VFE39_01880"
     CDS             complement(16998..17534)
                     /locus_tag="VFE39_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET6257376.1"
                     /translation="MTEKHRITVTVNGRAHRAEVEPRTLLVDFLRDELGLKGTHIGCE
                     HGVCGTCTVLLDGRSIRSCITFAVQADQAQIRTVEGLASGPDLHPLQDEFWHKQGLQC
                     GYCTPGMLMRACEILEQNPDPTPEQVREGIASNLCRCTGYQFITEAVVAAAGRMRNEH
                     PWDPTRPTAATTVSEERP"
     gene            complement(17541..18401)
                     /locus_tag="VFE39_01885"
     CDS             complement(17541..18401)
                     /locus_tag="VFE39_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET6257377.1"
                     /translation="MKPPPFRYAVPTTVAEAVGLLVEHADAEPRVLAGGQSLVPLMNF
                     RLAQPGHLIDLRRVPELDGIRLDGDTLVIGAMTRQSAVEDSPEVALVAPLLAEAVGFV
                     AHRPIRNSGTIGGSIAHADPAAELPAVALALDAEIVAVGPDGTRSIPAAEFFRGPFST
                     ALAPDELLTEVRLPHWRGGHAFVEFARTHGSFAIVAVAALVEMADGRVARASIALTGV
                     APVPIRAAAAERALEGAVPDAAAAADAAVEGLAPASDVHAGSASRVQLARVHVRRAIE
                     RAVSRAQDRR"
     gene            complement(18583..20487)
                     /locus_tag="VFE39_01890"
     CDS             complement(18583..20487)
                     /locus_tag="VFE39_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6257378.1"
                     /translation="MVATEVQASLRSWMSAVSEIARAVNAAEPLHDVLTRVAAQACAL
                     IGFEYCAVMLTDPDGEWLQVAGWCGLSPDYLAQLSAEGSLQVHPSGSLLDTPAARACR
                     EGRTVTVPDVRTATDYGRLQLLAPTQGYRSLVAAPLPAAEDDPPASPAGGAGVAGVLV
                     GYSVTAREFEPPEVELIELLAGQAALALETSRLRTAQQDVIGELSGANDELRRGRAVL
                     DWAEQQHRELMQLVLDDVGLAGLVASLAGTLAASVTVEDADGRVLARAPDDGYRPPPT
                     AAARRRSPAREALEALTRRYEVVQIPVLAPGGPVVRGAPAPTRREMAWVAPVVLGQEL
                     VGRLWVTAPPAVPAPVQLRVIERFALVVALELLKARHLVAAEGRLSGDLIADLLRPDG
                     PGRPHAVLDRAAALGHDLARPHVVAVLTLDGPIPPAVRLPELVRAAAGPDARPLVGSY
                     EDDWVVLLPAEPDPGDVLRRVHAHTAHAAGSGCVVTLAAGPTACAPEDFAAAYGVARG
                     AVQLRRATRPGGLVDVRQLGLSALLLETGAPDALRRFAQTLLRPLAAHDAQRGGDLVA
                     TLRAWLGSGCSTVATAEALTVHPNTVGYRLGRIEALTGRSLRGVDTRLELQLALTVRD
                     IVQLGDPDHR"
     gene            20574..21755
                     /locus_tag="VFE39_01895"
     CDS             20574..21755
                     /locus_tag="VFE39_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HET6257379.1"
                     /translation="MTTGASPVRTRTDRTATAERRAADRMAAAAAAWLEALDAEQRAL
                     AHWPAPGADAETEAERLQWYYTPTDHGGVPIGAMHPRQQSLAMQLVAAGLSTPGYVTV
                     ATVMGLENVLDHVEDWGVDWGRERGRDPGLYYLRVFGDPGGQAPWGWRFGGHHVSLNN
                     LVVSRAVRSTTPCFLGADPAMSPLLGGTSLHPLGAVEDLARELVRSLAPEQAARAVLL
                     PRAPSDIIGGNRARITQGDEMLRLPDIWRGHFREQRLTDRLSEMDIRAESSSGFDATD
                     HSTLALTVVPKGLPAAELEVDQRALLRALLDTYLGRVPDGLAPGPLDDPALDAVHLAW
                     AGGTEPGEPHYYRLQGPRFLAEYDNTQREVNHAHSVWRDPEADFGVDTLAAHRAIHHH
                     G"
     gene            complement(21743..22834)
                     /locus_tag="VFE39_01900"
     CDS             complement(21743..22834)
                     /locus_tag="VFE39_01900"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6257380.1"
                     /translation="MTATLLLAADTVVTDGLVGPGWVEVAGERISAVGAGPSPRTPDV
                     NLGDAVLVPGFVDMHVHGGAGAAFPDGDPEEAMRAVRFHRAHGSTTMVASLVAAAPAE
                     LLRAVDALAELVADDELAGVHLEGPWLAEARCGAHDPRQLRDADPGELDRLLRAGQGA
                     VRMVTLAEGSLPAGQWMEWNRPIRIRASALVVHAAMDVPWSAPMTARPALRAACAGRQ
                     VLPSGLVRATGLGRAILRIVAAPGRRREVDHMRAKVIVLACATLLITSCGGTVGGGTG
                     GGGGAGSTEPVKRHARAGARGLHVVDHSRLPDGRNRHDHGDSHAAVHRPHLLTRVGRV
                     DGDPGPEPPGEPGRLGRHDPRNCAAPQPW"
     gene            complement(22831..23901)
                     /locus_tag="VFE39_01905"
     CDS             complement(22831..23901)
                     /locus_tag="VFE39_01905"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET6257381.1"
                     /translation="MTTVAAAQRPGRLMAAEIAEQPAAWRRLLVDGHAAGLDAAAALI
                     RSRAPRTVLFVARGTSDHAALYAKYLAEIRLGLPAGLVSPSTMTAYGARPDLRDVLMI
                     GISQSGGSPDLVRSIEVARAQGALTVAVTNNADSALARAAHAHVDVLAGAERSVAATK
                     SYTAQLLALYLLLEQVRDGRDGGAGPAAALPDLGDALLARSAEIAAIAQRYRFAQRMI
                     TTARGYSYPTAREAALKLMETAYVSAQAFSGADLLHGPLALVDPQVPVLAVVAEGVGG
                     AAMEHVLPRLAERRADVCCVGHPDAVARVAVGFSLPAGVPEALSPLLEIIPFQLLALH
                     VAVDRGGDPDAPRGLSKITETL"
     gene            complement(23898..24851)
                     /locus_tag="VFE39_01910"
     CDS             complement(23898..24851)
                     /locus_tag="VFE39_01910"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HET6257382.1"
                     /translation="MQLRSVIGIDIGGSKTHGVRSADGVRVAEAVAGSANIAAVGVAE
                     AGRQLDALLADLDPAGVDAVCAGAAGADGTASVGSLERLLADRVPGARVLVVHDARLV
                     LAAAGLDDGVALISGTGSVAWGSRSDGTEARAGGWGHLLGDEGSGYRIALDAVRHALD
                     RMDAGLPVDTLTAALLEHCGLTTREQLIDHVYDTQDRRYWAERAGAVAELADRGDAAA
                     TAILEVAADALAALATRVLDRLDGHGPVVLGGGVAVHQPRLQRAVRDRLPDVEVRVLT
                     EAPVTGAVRLAAALADPEQGSHPAQEGAVPELARTGREPRA"
BASE COUNT         3717 a         8976 c         8711 g         3578 t
ORIGIN      
        1 ccgacggcgt ggtgcaggag gtccgatgac gaggaagcgg tacgaggacc tgacccgggc
       61 gggccgcaac cgcgtggtga cagcgaccct gctgcgggcc gtcgcatcgg ccgcgctgct
      121 ggtgctggtg tactacttcg ctccgctgga cgcggcgctc aacgccgaca ccgtggtctg
      181 gttcgtgttc gcgctggtgc tcgtcggcgc ggtcatcgcc tggcaggtgc gggccatcct
      241 ccggtccgac gtcccacggc tgcgcgcgat ccaggccgtg gcgatcggca tcccgatgct
      301 cctgctggtg ttcgccgcgg tgtacctgct gatcgacacc gaccggccgg gcagcttcac
      361 ggagccgctg agccgcaccg atgcgctcta cttcactctc accgtcttca ccacggtcgg
      421 tttcggggac atcaccccga agagcgagct cgcccggatc atcaccatga cccagatggt
      481 cgtggggctg atcgccgtcg ggctcgtcgc gaggatcctg ttcagcgcgg tgcagatcgc
      541 ggtggcgcgg cgaaacggcg ctggtccgct cgggccggcc gccccggacc acgatgacgc
      601 ccgacagccg gacccttccc ccgacaccga cgccgcggga agcggatgag cccgagcgac
      661 cctgacgaac gtgcctgtag cacttcacct ttggtcggtg atgcgcctac cgtcgccacg
      721 gcgggcgccc agagagtcga ggcgtcgcgg gccggcgacg tgaccgagag tcttcgcccc
      781 gtcggcaccg accgaccgca tggatctggt ccaacaggga ggtcctgacg tgagtcagcc
      841 aacggtggat ccaacgttct acaggtcccc ggccgaggcc gcggctgcgc cggtcgagga
      901 gctcgcgtac gtggcggcat tcgaccgcgg cggcacgacg aacgacgcga tgaccgtggt
      961 cgatctgaac ccggactcgg acacgtacgg gaaggtcgtc ggctggaccg acatgcccac
     1021 ggtgggcgac gagctgcacc acttcggctg gaacgcctgc agcagcgcgc tcaagcacga
     1081 gggccacgac atggacggcc tcgcccgccg gtacctgctc gtccccggcc tgcggtcgtc
     1141 caacatccac gtcctcgaca ccggcccgga cccgcgcaac cccactcatg tcaagaccat
     1201 cgacagggcg gacctctcgg ccaaggccgg ctactcccgc ccgcacaccc tgcactgcgg
     1261 gcccgacggg gtgttcctca cctgtctggg cggcccggag gggaacgacg acgggccggg
     1321 tggcatcgct ctgctcgacc acgagacgtt cgacgtggtg cgggcgtggg aaacggatcg
     1381 cggcccgcag catttccact acgacgcctg gtggcacctc aaccagaacg tgctgatctc
     1441 cagcgagtgg ggcagcccct cgatgatcga ggacgggctc gtccccgaac tcctcctggg
     1501 tcagaagtac ggccacgcca tccatttctg ggacctcgca acgggccggc accagcaacg
     1561 ggtcgacctg ggtgcccagc accagatggc gctggaggtc cgccccgcgc acgacccgga
     1621 cgccacctgg ggattcgtcg gggtggtggt ctccaccgag gacctgtccg gatcggtctg
     1681 gcgctggttc cgtgacggcg acgaatggaa ggtggaaaag gtcatcacca tcccggccga
     1741 accggccgac ccggacgtgc tgccccctgc actgaagccg ttcggcgcgg tgcccccgct
     1801 cgtcaccgac atcgatctgt ccgtcgacga ccgctttctc tatgtctcct gctgggggat
     1861 cggtgagatg aagcaattcg acgtcagcga ccccgcccac ccgaggcaga ccgggtccgt
     1921 ccggctcggt ggcatcgtcg accgcgcacc acaccccgcg cggccggacg taccgatcgc
     1981 gggcgcaccc cagatggtgg aggtcagccg cgacgggcgc cgcgtctact tcacgaactc
     2041 gctctacggc gcgtgggacg accagttcta ccccgacggc gtcggggcgt ggatggccaa
     2101 gctcgacgcc gatcccgacg gcggcctgag catcgacgag aacttcttcc cgcacggcga
     2161 ggagttccgc ggtcggcggg tgcaccagat ccggctgcag ggcggcgacg catcgtcgga
     2221 ctcctactgc taccgctgac taccggagag cggatggcag gcgtgccgcc tcagctggtg
     2281 cggcgcgcga tcacgtcacg gatcgcgctc agatgctcgt tcatcgcggc cgccgcagcg
     2341 tccgcgtcgc gggccagtac tgcctggtag atcgcctgat ggccctcaag cgagcgcagc
     2401 ctgccctccg gcgtggcgtg cgagtgcctg cgcaacgtct cggtccaccg gctcgtcatc
     2461 gtgcacagcg ctacgagcag cgggttctgt gccgcgtgcg ccagcagcag gtggaactcg
     2521 acgtccagct gcagggacat gtccgcatca gggtgcagcg ccgagcgggt cagcacgtcc
     2581 tccagctgca cgagattgga cggccctgac cgtaccgcag ccgccgaggc gatccggggc
     2641 tcgacgatca gccgcagctc tgtggcgtcg tccagcgtga cgtcggtgcc gtccagctcg
     2701 gagtacaact cggtgaccga ctccgtctgc tcggcgacga tcgtgccccg ccccgtgcgc
     2761 cgctccacca ggttcttggc ttccagctcg tgcatcgcct cgcgcagcga accccgggac
     2821 accgacagtg tcgcggccag ctcccgctcc gggggcagcc gctcccccgg cctcagcacg
     2881 cccgtcgcga tcagccgctc gaaatgcgcc gcaagctgga cgctgacgct gcgggcgcgt
     2941 tggggcatgg ggggcaccga ggctggctgc acggtctgag catagagcca gggcaacccc
     3001 atatggtccc ttcgtcagac cagtgttcca ccactgtccg tcgcgtttct gcgcggtaaa
     3061 tatcaggtca gatccctgat catgcccgat ttcgcggtgg agaccgccat acagtgcctt
     3121 ggtccgacca acggactaat gatccgctgt cgccacctcg aaggactcct ccgccatgtt
     3181 gaaccggctg ctccggcccg ccgcgctcct cgcctcggcc ggtgccatca ccctgctggc
     3241 gaccgcctgc ggtggcggct cacccgccac ctccggcaac gcccccgcct accccgcgct
     3301 cgcgtcgtcg ccgacctgct cccagctgcg cacgcagaat cccgacctgg ccggcaagac
     3361 gctgacgaac gcgatcaacc cgcacacgcc ggggtacgag tcgatctcgg tgtccgaccc
     3421 cacgaagtac gagggcttcg acatcgacct cggcgaggcc atcggtgagt gcctcggctt
     3481 caaggtcagc tacctgccgg tggccttcga cgcgatgctg ccgaccctgc agagcgggca
     3541 ggccaacttc atcatctccg acatctacgc gaccaaggaa cgggccgcct cggcggactt
     3601 catcacctac tcgaaggtca acgacggcgt gctggtcgcc gccggcaacc cgaagaagct
     3661 caccggcatc gacgactcgc tctgcggcac caccaccgcg ctgaacaagg gcttcgtcga
     3721 ggtgccgttg gtggatgcgc agagcgccaa gtgccaggcg gccggcaaac cgcccgcgca
     3781 gactgcattg ttcgacaaca acgccgactg cgtgcaggcg atcctgtccg gccgggcgga
     3841 ctcctacatc aacgacgtca acacggtggc ccgattcgtc aaggaccagc ccgacaagct
     3901 ggccagggcc accgccgtga ccctgcccta ctcgatcggc atcgccgtgc cgaagggcca
     3961 gctcccgatc cgcaacgcct tcaccgccgc actgaccgag atccagaaga gcggcctgca
     4021 gactcagctg tccaagaagt gggagctgga cgagtccgcg atcgaggcgc cgtcgctcgt
     4081 caccgcgagc tgatcgagct gatggagagc ttcctgcact acctgtccat gccctacctg
     4141 ctgaccggga tcctgatcac cctgcaggcg acggcactgg gcttcttcgg cgggttcgcc
     4201 ctgggcctgc tgctcgcagg catggcgctg agcaggaacc gcgtggtctc cggggtcgcg
     4261 cgcacttaca gcgtgatctt ccggggcacc ccgctggtgc tccagctcgt gttcgtgttc
     4321 accgcgttgc cgcacgcagg cgtcgtgctg agcccgatca tggcggccgg ggtcgccctt
     4381 gccgcgaacg aggccacctt cttcgccgag atcttccgct ccgcggtgct cggcgtggac
     4441 aaggggcaga ccgacgcggc ccgcgcggtc gggctgcggc ccgggaccgt gctgcgccgg
     4501 atcatcgggc cgcaggcggc gcgtgcggcc atccccgcga tcggcagcga agcggtctcc
     4561 accatgaaga actccgcact ggcctcgatc atcgccgtcc cggagctgac cctgcgcagc
     4621 aaccagctgg cgtcggccac gttcgactac ttcagcatct tcttcgcgtc cggcgtgatg
     4681 tacctgatcc tcaccggtgc gatcaccggc ctgcagctga ccatcgagcg tgctctcgac
     4741 ctcagccgcg tcgggagacg cggccgcggg cgcccggcag taccgagcac ggtcaacggt
     4801 gcctccgtca tcgagacgcc actcgccacg aaagagctga ccgccacaag aaaggtgaac
     4861 ggcgccgtgt cgcgggccga ggccgtcggc cgggccaggg tcgcgctgac catcaacgat
     4921 gtccacaagg cgttcgccga ccacgaggtg ctacgcggta tcgacctcga gatccgcaag
     4981 ggcgaggtcg tcgcgttgct cggtcccagc ggatcgggca agagcacgct gctgcgtctg
     5041 atcaaccacc tggagaccct ggacacgggc aagatctcgt tcgacggcat cgaggtcggc
     5101 taccgggacg ccggcgtgcc gctgccggag cgggaggtcg cgcggcagcg ggtggacgcc
     5161 ggcgtcggca tggtgttcca gcatttcaac ctgttcgggc acctcacagc ccgggagaac
     5221 gtcgcggtac cgctgcgctg ggtgcatcgg gtgcccaagg acgaggcact ggccgcggcg
     5281 gaccacctgc tgggcagggt ggggctcagc gccagggggc acgcgttgcc ccgccagctc
     5341 tccggcggcc agcagcagcg cgtcggcatc gcccgagcac tcgccggtcg accccaggtt
     5401 ctgctgctcg acgagccgac ctcggcgctc gacccggagc tggtccagga ggtgctggag
     5461 gtagtccgct cgctcgctgt ggacgacggg ctcacgatgc tgatcgccac ccaccagctg
     5521 ggcttcgcca gggacgtcgc cgaccgggtg gtcttcatgg ccgatggcac ggtgctcgag
     5581 cagggaccgc cccaacaggt gctggacaac ccgaagcacc cgaccgcagt ccggttcctc
     5641 cgggccgtac acaccgtgga ggtgtaggcg tgaacccaca caggcagtcc ttcagcttca
     5701 cacccgaaca gggcgtgcca cccgcggtcg ccccgttctc gcacgtgacc cagtggggat
     5761 cgatgctctt cgtcaccggg cagatgccca ccgacccggc caccggcgcg ctggtggccg
     5821 gcggcctggt cccccaggcc gagcaggtcc ggcgcaacct actcgcctgc ctcgccccgt
     5881 tcggggcgaa cctcgaccac gcgctcatgg tgcgcgtcta cctcaccgac ttcaccgagt
     5941 tcgaggcgtt caacgccctg taccgcacct ggttcaccac tgccctgccc agtcgcacct
     6001 gcgtcggcag caccgcactc gccgtgggcg ccgaggtcga gatcgacctg atcgtcggcc
     6061 tgcccgggca caacattgga gcctccgcat gaacctgacc ttgctgcagc cgggcgaggg
     6121 caacatcccc ggccgacacc tgctcgcctc gacaccggag acggtgagct ggggctggct
     6181 gccaaacgag aagtccactc cctgcttgac cgtcggctct ggcgagacgg tcaccatcga
     6241 cacgctcagc cacgagggca tcctggacga ccagggccgc gacccggtcg agttcctggg
     6301 ccgctacggc gtcgaggccg ccgacgtgtt gcacgatgcg aaggcgatcg ccgccagcac
     6361 cattcccaac gaacccgacg acggcccgca cgtggtcacc gggccgatcg agatcgccgg
     6421 cgccgagccg ggcgacgtcc tgaagatcga cgtcctcgaa ctgctgcccc gcacgcccta
     6481 cggcttcgtg agcagccggc acggctacgg cgccctgccc ggcgagctgc ccgagggcgc
     6541 gcagagcgtg ttccagttct gccgggtcgg cgtgatcgac gggcgaatgg tcagctggat
     6601 ggccgccacc gacggacggc aggcctgctt cccgctggat cccttcctcg gtctgatggg
     6661 tgtggccccg gccaccagtg aaccggtgca ctcggtgccg cccggtgact tcggcggcaa
     6721 catcgacgtg aagtgggcgc aggtcggcac ctcgctctac ctgccggtca cggtgcccgg
     6781 cgccctcttc tacaccggcg acccgcacta tgcgcagggc aacggcgaag tcgcgttgac
     6841 cgcgctggag gcatcgctgc gcgccaccct ccggctcacg gtgctctcgg gtgcggaggc
     6901 gagcacggcg atgggcagcc tgcgcaaccc gctgctggag acgccgggct actggatccc
     6961 gacggggatg gacgaggacc tccgcgaggc gatgaagaaa tgcgtgcgcc acgcgctcac
     7021 gttcctgatc gagcggttcg gcttcgaccg gtcgaccgcg atggcctacc tgtctgcggc
     7081 gggcgacttc gagatctcgc aggtcgtgga cgtgaccaag ggcgtgcact gcatgatccg
     7141 caaggccgac ttcgcgcagt ggtactgagg ccccgctgat cggagaaccc gcatcggctg
     7201 acgcttcagc ccgccgtccg ccacctgccg gccacccagc ccccgaccgt ccgagccgac
     7261 tggagcgccc gcgccacggc ctcccgatga cgctggtcca tcgtgtccct gagccgtagc
     7321 gcagcgagtc ggtccgtgtc ccggcggcag gtgcagtcag caggaatgaa ccacccacag
     7381 cgtccgcaga tcatcgctcg tctccttcac agccatcaga tcggacagca caggcacggg
     7441 gcaggaacgc caccgcctgc ggttcagccg cccacacccg caggcgagca gcgggatgcg
     7501 gccgagccca ttacgcgccg cgaggatcgg ggcgagtgtg cagaatctct ctcacgctcg
     7561 cctccatccc tcgttgccca gtcagcgtcg cgggaatagg tgtggcacgc atcaccgcca
     7621 gcggccagac gttctggggg ctgacgggaa gagtggggcg cgccacccgc tgcgctcagc
     7681 cggggaggat cctgacgccg cccgcagcga ggtcggccac gcactgccac ggtccctgcc
     7741 actggtgcgc cgaccagccg cgctcacgtg ccgcggccac gttcgcgggc cggtcgtcga
     7801 agaacatcag ctcaccgccg gacagcccga gggcgtcctc gaccgcctcg taggcgccgg
     7861 accgcggttt cgcgacgccg gtttcgcagg agaacacttt cgtcgggaac aacgctcccc
     7921 atccggaact gcgcacttct gccgcaagcg agtgcggcgc gttggagaac aacgccatcc
     7981 gaacgccgga gtgcgcgacg cgcacgacga gcttcaccag gcgaggcgcg agctccgccc
     8041 agcgctgcgc gtccccccgg tccaagcgcc gcacctcggc cggggtgagc gttctcccga
     8101 cgtccgcccc gacctgtgtc cagtactcgt cgacggcgcc gccgaggtcg tagccatccc
     8161 ggtaccgcca gtaggccgtc gtgaatccgg tgacgtcgac gcgggcttcc gccgccagcg
     8221 cggcgatgcc gccctccggc tcggcgagga cgccaccgac gtcgaagacg acgaccgcgg
     8281 gccgggaacg tcgcacaccc gcagggtagg acagcagccc cgtcgaactg cgtgccacgc
     8341 cgagtgccct acaagcgatc acccgccagg gagaaccggc acggccgagg cgccgccgac
     8401 ctgggcggtc tggcccgtcg cacgagacgg gacggcgctg cgggcgtgcc cgactgtggc
     8461 ccggctcacc caacgagggt gatgcccacg gcgggcggag tccgcagagt agccgccgac
     8521 gatctgtcca gaggagggaa cccgatggcg acgttgacgg tgtgggagtt cgacaccgcg
     8581 gacggcgcgc agaacgcgct cacaactctc gagcggctgc agaaggagga actgatccgc
     8641 gtagtcgacg tcgcggtggt gacctggcct gccgaccgca agaagccgcg gacccaggac
     8701 gccgtcaccg accgcgtcgt cgagaacttc cggggcaccc gtgccgagct gatcagcacc
     8761 aacctctcgg ccgatcagga ggcgaagctc cgagaggcct tcgaggacgc cgaccacgcg
     8821 ccggtctgac ctccgcgggg cagtcaggac ggcggacgcc gttggcggcg tccgccgttc
     8881 tggccctcga ccagtgggca cccgcagcga ctcacccgtc acgggtgagg ggtgaatggc
     8941 tgcggcgagc aacgatcgaa accaacatgc acgatgagga ggagttccga tgtcgacatc
     9001 gccccaggcc actcccggca atgcgaccgg ctacggcgac gccgccgggc gcgcggccgc
     9061 cgaaacctcg ccctggaaga ccggattcgt cgtcttcgcc ggcaccatca tgatcgtcgc
     9121 aggcgtattc caggccctcg ccggactggt agcactcttc cgcaacgagg tctatgtcgt
     9181 cgggctgaac tacgtgttct ccttcgacgt cattacctgg ggttggattc acctgatcat
     9241 cggcatcgtg gtcgcggtgg ccggcgccgc ggttctcacg ggccaattgt ggggacgcgt
     9301 cttcggggtg ggcatcgccg ccgtgagcat gctcacgaac ttcatgttca tcccctacta
     9361 ccccgtgtgg tcgctcctga tcatcgctct cgacgtcttc gtgatctggg cgctgtgcac
     9421 gtacacccgc gaccgcagcg aagccgtcac acgctgatca gcacgcactg gccgagctgc
     9481 cgcccccacc cggggcccgc gacgatcctt caggagctgc gcgatgatga tcgggatact
     9541 catggtcgcg ttcctgccac cgttgatcat cgccgtggta ctcctgctgg agcgggtcga
     9601 ggagagcctc ggcgcgaccg gccacgatcc gcgcccggct tcgcccaacc cgggtgcggc
     9661 cggccacctc ggatccgcgg gatccaggac cgattgacgg cccgctcgag ggcggcaccg
     9721 gactcgtgag ttctgatgtc cagaagcgac gaggttttcc cgatcggcga cgatggacgt
     9781 atgaggatcg gattcggcgc atcggtgtca ggggcctggg cgggcccgga gaatcttgcg
     9841 gccttcgcgg agcgggccga ggagctgggc tacgactcgc tgtggacgtt ccagcggctg
     9901 ctcacgcccg cggacccgga agcgctcgat gtggtctacc gcagcgtgct cgacccgatg
     9961 gtggcactgt cctacgtggc cgcgcggacg tcgcggatcc ggctcggcgt cgccgtggtc
    10021 aacctcccgt tcgtcagccc ggcgtacctc gcgaagcagg cctcgacgct cgacgtgctg
    10081 tcgggcggcc gactcgacct cgggctcggc atcggctggc agcgcgagga gttcgcggcc
    10141 agcggggtga ccccggagcg ccgcggcgcc cgggccgagg agtacctcga ggcgctgcac
    10201 acgctcttcc acgacgaggt cagcgcgttc gacggcgagt tctacacgtt gctgccgagc
    10261 cggatggcgc ccaagccggt cggcccggcg gtgctgctgg gtggcgcggc ggaggcggcg
    10321 ttgcgacggg ccgggcggct ggccgacggt tggatgagcc gcagcgccac cgatctgacg
    10381 aagatcagcg ggcagatcgc ggtcgtccgg gaggcggcgg agagggcggg caaagacccg
    10441 gctggcgtcc ggatcgtcgt ccgcggggtg gtccgggccg gcggcgactc tgaccagcgg
    10501 ctggccggct cgtacgacaa gatccgcgag gacaccgcct ggctcggcga gcagggcgtc
    10561 accgaggtgt tctacgacct gaactgggat ccgcagatcg ggtcaccgga cgtcgacccc
    10621 gccgccgcca ccgcgcgcgc cgaggagatc ttgcacgagc tcagcccggg ccggtgaccg
    10681 gtcgagcggc gggaccgcgg tggtgacagc gtgcgcctac tcggcccagc tgactgcatg
    10741 atctcctagc ggagggcccg gccgcggcgg tcatccatcg tcatggcctc caccagcgca
    10801 tgcggatcga gcaggcccgc ccgtaccgga ccagtcaggt cccccgcctc ccgcagcgcc
    10861 gtgaccgcgc ctccacgacg acggtcgaac gacacaccgt cgtcgcgtcg acctcgcccg
    10921 cctccggctc gtgcgacccc acggcgacga cggtggtgcg cgagccagca gggctccgtc
    10981 gaacaggggt gtgcgcgcgg tcgtggcgca gacgaccacg tccgcggccg ccacgtcggc
    11041 gctcggcgag atcgctcaca gctggaaacc ctcggggaag gggtcgtcgg tgtccaggaa
    11101 aactgcgcgg tgccggtgag ccacacgcgc aacaacgcgt gaccgccacg accgctggca
    11161 gctctgcgac agccgtcttc tcgacgacgc ggtagatgaa gtgggtgccg aggtgctcag
    11221 cgcggggatc tcgacaccgg tcgcgcgggc cgacgcccgg tgcaccgttc aggcatcacc
    11281 cggcgtgtgg cgtgatggcc aggctggcga gaacggcgtc ggctgggccg gtggcgaggt
    11341 gctggtcggg atcgacgtga cggagcaggt cggcgatcgc gtcgatgctg acgccggcaa
    11401 tgtggagctg ggtgccgcag ctgtcggcct gggcacgcag tgtgcgcagc tcggtcgcgg
    11461 cgtgggcgtc caggcagccg accgaggaca ggtcgaggat gacgtgcgac gcgcggtgga
    11521 gctgctggcg gacccgtaac atcagcagcg gcgcagttga gcgacgcaac gggccggcga
    11581 cccacacgat gaccgtgtcc gggctcggcg catgtagccg gacatccagc ggctcgtccg
    11641 gttcctccgt gatcgtgaaa gccccccgcg aggagtggcc catcaccgcc gtccacgtct
    11701 gcgccccgaa ccgatcaagg tgggtgtggt ccacgtcggt gtcacctggc atgccgaccg
    11761 cccggccgag aggtgccggc tgacgcgcgg tagatcgcga acaggtcggg caacgtcgcc
    11821 tcgtccagct tggcggcgac gggcaaagtt ccgttcgggt tgatccgcag ccatccctgc
    11881 cggccctcca ggagtcgggc ggcatcggcg agcacgccca tcaccgcggg gtcacagcca
    11941 tcggcctcgg cgaggtccac cagcaggtag cgcaccccgg caaccagcag tccctccacc
    12001 cgctgccgca gctcagcagc attgtcctgg cacagcgcgc cggccacctc gagcacgccg
    12061 tggtcccggt ccggctgaac cgacctgatc gctggggcag ccgggggcgg gctcgccgac
    12121 gctgtgaggc gcccgagcgg gctcgtgatg gtcattcggg tccccaatcc ccattgttca
    12181 ggatctcgac tctcatgaat tccaaatttg acattacgac gggaaacaac ccggcgcaag
    12241 cccccgtgcg ggcctatagc tgcccttccc ggctgaactt caccctgttc cacagcaccg
    12301 ttccccgcgg ccgcgggatg ttgactgtcc acactgtcga tgtcgatgat ccttctcgaa
    12361 gacccgggac gaaagaccat tgcggagcgg gttgtgcacg gggtaagtgt tttaccactg
    12421 cgtcacccgc tccggcggtt agcgcaatcg ctggggcaac tgggggtgag cattggtcca
    12481 gacggtggtt cgagtcgtcc ttcctggtgc cggtgccggc cctggagtgg acggatcgac
    12541 cgctcgaggc gaatgtcaac gccgagagcg aagatcgtgc cgcccgcgac cgaggcgacc
    12601 cggtgatggc ccgggacgga aacgtacgcc ggacctggga cagcgtgcag cggacatatg
    12661 agggtgtgcg tctcgaccac gaccagccct gatcgagtga gggccgcccc cacctatttc
    12721 cggtcaggag cagtttcact tcggcgagga gttcctggcg atccggtgat gcgagaagac
    12781 cgttacccag atgcccgact cgggtgcgcg ggagaccgtt tcccgataga ccaggccgcc
    12841 ggccgggacc gggaccggga ccgggaccgg gaccgggcgg agctgccccg ggcttgagga
    12901 atcgccggtc gcaccccgtg gcgacgcccg tattgtatgc ctcgctcgtc gcgagcagga
    12961 caggggacgt catgcccacg accgtcgtcc accggccgcc accggcgccg agcctgccag
    13021 aacgggccga cgcactccat cagtcgacga gcgcgaccgc ccgggtgggg ccgccgtggg
    13081 cgccctgcag cttcaggggg aacccgacga acgtgaagcg gacgttcacc acccggtcca
    13141 ggttcgccag gttctcgtag tgggtgatgt gctcgcggcg gcacatcatg tggcaggggt
    13201 agctggtgct cgtcgggttg tccggggtcg ggctgtccac gccgaacgtt ttcaccccac
    13261 gccgcacgat ccactcactg gccgactcgc cgagcccggg gaactcgctg aggtagcgct
    13321 tgtctcccgc gtaccggttc caggtcgcgg tgtggaacag cacgatgtca cccctgcgca
    13381 cgtcgacggg cgactccgcc tccacccggt cgagatcctc ggcggtgatg tcggtctggg
    13441 gcttgaaatc gctgacgtcc aggcacaccg cagtgccgta gaacaggtca agggacatct
    13501 tctcgatcgt ctcggcagac ggatcggggt ccaggtgcga gaacgagtcg acgtgggtcg
    13561 gcccgttgtc gttgagcatg aagccggtgg tctggaacga gaagccgctg tcgaaccggg
    13621 gggccgtctc ctcgtgtgtg acgtgctcga actgcacagt cttgaggtgg cccgggtaga
    13681 ccttcatgcc ctcgtagatg tcctgcgaca ggtcgatgat tgtcaattcc actcccacgc
    13741 ccaatgagcg actagtacat gacgacgccg gcggtgacgt tcacgtcctc gccggtgatt
    13801 gccgcggcac ccgcggaggc gaggaaggaa cacgtccgcg ccacctcgtc ggcctccacc
    13861 agccggccca gcggggacga tccggtgaac acggccagtg cctcgtcctc gctgatgccg
    13921 cgcgcggcgg cctgcttgcg caccacgtcc tcgatccgcg ggccggcgac cgcgccgggg
    13981 cagaccgcgt tcacccggat gccgtacgga ccgagctcca gggccagggt gcggaccagc
    14041 ccgatgaccc ccatcttcga cgcggcgtaa ggggtgcgcc cggtcagcgg acgcttccct
    14101 gtcatcgacg agatcgcgat cagactcccc tcccgagcgt tgatcatcga cgggacgaac
    14161 gcccggaagg tgaggaagac cccgtccagg tcggtggcca cgcagtcgcg ccagtcgtcg
    14221 agggagatct cgtgcagcgg cgccgtgggg cccgccaccc cggcattggc gacgacggtg
    14281 tgcaccggcc cgatcgcggc gaccgcctcc gccatcgccg ccaccgaccc ctcctcccgg
    14341 acgtcgcagg ggagcgcgac cgccgtgccg ccggcctgct cgacctcggc gagggtgtcg
    14401 gcgagcgtcg ccgggtcgcg ggcggtgacg gccacgtcga acccgtcggt ggcgagtgcc
    14461 acagctatcg ctcgtccgat cccccggttc gcccccgtga cgacggcgac ccgcgcgttc
    14521 acctgaccac cccgacacgg gccccgctcg ataccgacct gtgggtcatc gggctgcccg
    14581 cagggccgcg aggatgcgct cgggggtcat cggcaactcc gtcaggcgga cgccgagcgg
    14641 ggccagcgca tcgttcaccg cggcgttgag cgcggcgggc gccatcatcc ggccgccctc
    14701 gccgccgccc ttgatgccgt gcggggtgta cggcgacggc gtctcgacgt ggccgatcgc
    14761 gagctccggc acctccatgg cggacgggat gaggtagtcc aggtagctcg aggacagcaa
    14821 ctgcccgtcg tcgtcgtaga cgaactcctc gtagaacgcc gtgccgatgc cctgtgcggt
    14881 gccgccgacg atgtggccgg cgagcgagcg cgggttgacc agggtgccgc agtcgtgcac
    14941 cgcggcgtag gagaggatcc ggacgcctcc ggtctccgta tcgacctcga ccaccggcac
    15001 gtggcaggca tggcccatga acgggtagaa gacgcccagg tccttgcggt cggccgaggg
    15061 catcgtggtg taggggtggt cgaagacctt gctgtcctcc agacccgact ccatgtcgtc
    15121 ggggaagctg tgcttgaaca ggtgcagcat caccgcgatg tcggcgaggc tcttgcgctg
    15181 ctccggcacg cccttgatct ggtagccgcc gtcgacccat tcgagatcgt ccgggtcggc
    15241 ctccagcagg tgcgcagccg cacgcagcgc cttggccttg atcttcgccg tagcgccctc
    15301 cacggctccg gccagcatca cggtggtccg ggagccgccg gggccggtgg ccggcagccc
    15361 gttcttgctg ccctggtaga cgatcgcgac gtcgtgcggg tcgcagtcga actcctcggc
    15421 gaccagctga gccaccatgg tctccgggct gttgccccag aacgcgcacg agtagagcgt
    15481 ggcggtgatg ccgccggtgg cgtcgacctt gagggtgacg ctctcgggca tgctcgtgac
    15541 gggcgcgccg ggttcgtcga accagaacca gaactcggtg gcggagaaga cgctgcgctc
    15601 ctgggcggtg atcaggccga tgccgatgtg gcgaccctcc tcgcgggcct tcgcctgttc
    15661 ggcgcgccaa tggtcgtact ccgacagcgc cagtgccttg tcgagcaccc tgtcgtagtc
    15721 acccgagtcg tagacgttcc ccgtcgggat gaagtgcggg aactcgcgga tgaagtttcg
    15781 cctgcggatc tccaccgggt cgaggtcgag ctcgcgcgcg gccttgtcca ccatctgctc
    15841 gagcatccag ttgttgacct ccgagccgaa gccccggtag gcgccctgct ggttcttgtt
    15901 cgtcagcacc gcacgtaccc ggtaccgcac gctgccgatc atgtaggggc ctacgacctg
    15961 ggcgagcgcg ttgccgtggt gcccgacacc gaactggatg taggcgccgt agtcgtcgat
    16021 cgtgtcgatc ttgaggccgc gcatcgtgcc gtcgcgcatc atcgccagct cgacctcgta
    16081 cacgcggtcg gagccgtggt ggtcgcagtt ggacatgttg tcgacgcggt cctcgaggaa
    16141 gaccacgggc tgcccgacga cgcgcgagca catccccgcg atgaccagcg gcttggtgac
    16201 gaacagtttc gagccgaagc tgcctcccgc gggcacctga cggacgtcga gcttgttggc
    16261 cgggatcttc agcgtggccg cggccatgaa caggtagctc gtgaagctca acgagttggt
    16321 gtgcacggtg agctcgccgg tggcgtagtc gaagtcggcg atcgcaccga cggtttccag
    16381 gggctggccg ccggagcggt gccagcggag gcggtccttg acgatcacgt ccgcttcggc
    16441 gaagtcgcgg tcgacctcgc cgaagtcgaa ggtgcgctcg taggcgcagt tcgtgccgag
    16501 cgtctcgtgc accagtggcg caccgtcggc cagcgcggcc tcggggtcca ccacgggcgg
    16561 cagcggctcg tactcgacgt cgataagccc gagcgcgtcc tcggccaggt accggctgtc
    16621 ggcgaccgcg acggccacgc cctcgccgac gtagcgcacc ttgtccacgg cgaggcagcg
    16681 ccaggggtgg ttggccgggt ccggtccgaa gtcgggcagc gggtcggtga gctcggcggc
    16741 ctgtgcaccg gtgatgacgg cgtgcacgcc gtcgagggcg gccgcggccg cggtgtccat
    16801 cgagacgatc cgggcgtgcg ggtgcgggct gcgcaggacg gcggcgtgca gcgtgccggg
    16861 cgtgacgagg tcggcgatgt agcccccacg gccgcgcaag aacttgggat cctcgacccg
    16921 gcggatcgac ttaccgaccc acttacgggt ctcggcatga gtgcggacag gtggtgcgga
    16981 cggacggtcg acggcagtca cggcctctcc tcgctcacgg tcgtcgccgc ggtcggcctg
    17041 gtcgggtccc acgggtgctc gttgcgcatg cgcccggcgg cggccaccac ggcctcagtg
    17101 atgaactggt agcccgtgca acggcagagg ttgctcgcga tcccctcgcg cacctgctcc
    17161 ggcgtcgggt ccgggttctg ctcgaggatc tcgcaggcgc gcatgagcat ccccggcgtg
    17221 cagtacccgc actgcagacc ctgcttgtgc cagaactcgt cctgcaacgg atgcaggtca
    17281 ggtccggacg ccagcccctc gaccgtccgg atctgcgcct ggtcggcctg cacggcgaac
    17341 gtgatgcagg accggatgct ccggccgtcc agcagcacgg tgcaggtgcc gcagacgccg
    17401 tgttcacatc cgatgtgcgt gcccttgagt cccagctcgt cacgcaggaa gtcgacgagc
    17461 agcgtgcgcg gctcgacctc ggccctgtgc gcgcggccgt tcacggtcac ggtgatgcgg
    17521 tgcttctcgg tcatctcggc tcacctccgg tcctgggcac gcgataccgc ccgttcgatc
    17581 gctctgcgga catggaccct ggccagctgc acccggctgg cggagcccgc gtgcacgtca
    17641 ctggccgggg cgagtccctc caccgcggca tcggccgcgg ctgccgcgtc cggtaccgcg
    17701 ccctcgagag cgcgttcggc agcagcggcg cggatcggga cgggcgcgac cccggtcaac
    17761 gcgatcgacg cccgggcaac gcgaccgtcg gccatctcga cgagcgcggc caccgcgacg
    17821 atcgcgaagc tgccgtgcgt ccgcgcgaac tcgacgaacg cgtgcccgcc ccgccagtgc
    17881 ggcagccgca cctcggtgag gagctcgtcc ggcgccagtg cggtgctgaa ggggccgcgg
    17941 aagaactcgg cagcggggat gctgcgagtg ccgtcggggc cgacggcgac gatctcggcg
    18001 tccagggcga gcgccaccgc gggcagctcg gcggccgggt cggcgtgggc gatgctgccc
    18061 ccgatcgtgc cgctgttgcg gatcggccgg tgagcgacga accccaccgc ctcggccagc
    18121 aacggtgcga ccagcgcgac ctcgggcgag tcctcgaccg ccgactggcg ggtcatcgcc
    18181 ccgatcacga gcgtgtcgcc gtcgagccgg atcccgtcca gctcggggac ccggcgcagg
    18241 tcgatcaggt gcccgggctg ggcgagccgg aagttcatca gcgggacgag gctctgccca
    18301 ccggcgagga cccgcggctc ggcgtcggcg tgctcgacga gcagccccac ggcctcagcc
    18361 acggtggtcg gtacggcgta ccggaacggc gggggtttca cgacggtctc cgaagcacag
    18421 cggcctcctc gacgcagtgg ggacaggtgg gcgcgcagcg gactgtgagc gtcgtcacgc
    18481 gctaacaccg atcctgcgcg ggactgcccc ggccagccat gtgtgccacc ccaggaaccg
    18541 cacgggaacg ctgggttcgc ccacaacacg ccgggccggg aactaccggt ggtccgggtc
    18601 gccgagctgc acgatgtccc ggaccgtgag cgcaagctgc agctccaagc gagtgtcgac
    18661 gccccgcagg ctgcggccgg tcagcgcctc gatgcgcccg agccggtacc cgacagtgtt
    18721 ggggtggacc gtgagcgcct cagcggtggc cacggtggag cagccggaac ccagccacgc
    18781 gcgcagcgtg gccaccaggt ccccgccgcg ctgcgcgtcg tgcgcggcga gcggccgcaa
    18841 cagcgtctgc gcgaagcggc gcagtgcgtc cggcgccccg gtctccagga gcagggccga
    18901 caggccgagc tgccggacgt ccaccagacc gccgggccgg gtcgccctcc gcagctgcac
    18961 cgctccgcgg gccaccccgt aggccgcggc gaagtcctcc ggtgcgcacg cagtcggccc
    19021 ggcagcgagt gtgaccacgc agccgctgcc cgcggcgtgg gcggtgtgcg cgtgcaccct
    19081 gcggagcacg tcgcccggat cgggttcggc gggcagcagc accacccagt cgtcctcgta
    19141 ggagccgacc agtgggcggg cgtccgggcc ggccgcggcg cggaccagct cggggagccg
    19201 gacggccggc ggtatcgggc cgtcgagggt cagcaccgcc accacgtgcg gccgggcgag
    19261 gtcatgcccg agtgccgcag cgcggtccag caccgcgtgg gggcgccccg ggccgtccgg
    19321 acgcagcagg tcggcgatca ggtcaccgga cagccgcccc tccgccgcca ccagatgccg
    19381 cgccttgagc agctccagtg ccacgaccag cgcgaaccgt tcgatcacgc gcagctgcac
    19441 cggcgccgga accgcgggcg gcgcagtgac ccacagcctg cccaccagtt cctgcccgag
    19501 cactaccggc gcgacccacg ccatctccct ccgcgtcggg gctggtgctc cccggacgac
    19561 cggccccccg ggtgccagta cggggatctg cacgacctcg tagcggcggg tcagcgcctc
    19621 cagcgcctcc ctcgcgggcg aacgcctgcg ggcggcggcg gtcgggggcg gacggtagcc
    19681 gtcgtccggc gcgcgggcca gcacccggcc gtccgcgtcc tccaccgtga ccgacgccgc
    19741 gagcgtcccg gcgagggagg cgacgagccc ggcgagcccc acgtcgtcga gcaccagctg
    19801 catcagctcg cgatgctgct gctcggccca gtcgagcacc gcacgaccac ggcgcagctc
    19861 gtcgttggcc ccggagagct cgccgatgac gtcctgctgc gcggtccgaa gccgggaggt
    19921 ctccagggcc agcgcagcct gcccggcgag cagctcgatc agctcgacct ccgggggctc
    19981 gaactcccgc gccgtcaccg agtagccgac cagcacgccg gcgacgcccg ctccccctgc
    20041 ggggctcgcg ggcgggtcgt cctccgccgc aggcagcggt gcagccacca ggctgcggta
    20101 gccctgcgtg ggcgcgagga gctgcagccg gccgtagtcg gtggccgtcc gcacgtccgg
    20161 caccgtgacg gtgcgaccct cgcggcacgc ccgtgcggcg ggggtgtcca gcagcgaccc
    20221 cgacgggtgc acctgcaggg aaccctctgc gctcagctgc gccagatagt cgggactcag
    20281 cccgcaccat ccggcgacct gcaaccactc gccgtccgga tcggtcagca tcaccgcgca
    20341 gtactcgaac ccgatcaacg cgcaggcctg cgcggcgacg cgggtgagca cgtcatggag
    20401 cggctcagcg gcgttgacgg cgcgcgcgat ctcgctgacg gccgacatcc acgaccgcag
    20461 cgaggcctgc acctccgtcg cgaccacctg tgcactgtcg ccatccgagg ggacgcaggc
    20521 aagacccgtc ggggtgctcc ctcggcgcgc ggagcgtcgt agtgtgcagc gtcatgacca
    20581 ccggcgcgtc cccggtccgc accagaaccg accggacggc gacggccgag cgccgcgccg
    20641 ctgaccggat ggccgccgca gcggcagcct ggctggaggc gctggacgcc gagcagcgcg
    20701 ccctcgccca ctggcccgcg cccggcgcgg acgcggagac cgaggcggag cggctgcagt
    20761 ggtactacac ccccaccgac cacggcggcg tgcccatcgg ggcgatgcac ccgcgccagc
    20821 agagcctcgc gatgcagctg gtggccgccg gtctgtccac ccccggctac gtcaccgtgg
    20881 ccacggtgat gggcctggag aacgtgctgg accacgtcga ggactggggc gtcgactggg
    20941 gccgggaacg cggccgcgac ccagggctgt actacctgcg ggtgttcggc gacccaggcg
    21001 ggcaggcgcc gtggggatgg cggttcggtg ggcaccacgt gtcgctgaac aatctcgtcg
    21061 tctctcgcgc ggtgcgctcg acgacgccgt gcttcctggg tgccgacccg gcgatgtcgc
    21121 cgctgctggg cggcacctcg ctgcatccgc tcggtgcggt cgaggacctc gcacgagaac
    21181 tggtccggtc gctggcccct gagcaggcgg cccgtgcggt actgctcccc cgggccccgt
    21241 ccgacatcat cgggggcaac cgggcgagga tcacccaggg cgacgagatg ctgcgcctgc
    21301 ccgacatctg gcgcggccac ttccgggaac agcggctgac cgaccggctg tcggagatgg
    21361 acatccgcgc cgagagcagc tccggcttcg acgcgacaga ccacagcacg ctcgcgctga
    21421 ccgtggtgcc caaggggcta ccggcagccg agctcgaggt cgaccagcgc gcgcttcttc
    21481 gggccctgct cgacacctac ctcggacgcg tgcccgacgg cctcgcgccc ggtcccctcg
    21541 acgacccggc gctcgacgcc gtacacctgg cctgggcagg cggcacagag cccggcgagc
    21601 cgcactacta ccggctgcag ggccctcgtt tcctcgccga gtacgacaac acccagcgag
    21661 aggtcaacca cgcgcactcg gtctggcgcg acccggaggc cgacttcggc gtcgacaccc
    21721 tggctgcgca ccgggccatc catcaccacg gttgaggcgc cgcacagttc cgcgggtcgt
    21781 gacgtcccag cctgccgggc tctccgggtg gctccggtcc cggatctcca tccacccgtc
    21841 cgacccgagt aagcaagtgc ggccgatgaa cggcggcgtg agaatcgccg tgatcgtggc
    21901 ggttgcgccc atcgggaagc cgagagtgat cgacaacgtg tagtcctcgc gcacctgctc
    21961 gggcgtgccg cttcaccggc tcggtggacc cggcgccgcc cccgcccccg gtgccaccgc
    22021 ccaccgtgcc gccgcacgat gtgatcaaca gcgtcgcgca cgccaggacg atcaccttgg
    22081 cccgcatgtg atccacctct cgccgccgac caggtgcggc gacaatacga aggatcgcgc
    22141 gccctaggcc cgtcgcgcgg acaagtccgg acggcagaac ttgtctgccc gcacaagctg
    22201 ctcgcagggc cggtcgtgct gtcatcggcg cggaccacgg cacatccatc gcagcatgga
    22261 cgacgagagc tgaggcacgg atgcggatcg gtctgttcca ctccatccac tggccggccg
    22321 gcagcgaccc ctcggccagc gtcaccatcc gcaccgcacc ctggcccgcg cggagcagcc
    22381 ggtccagctc gccggggtcg gcgtcacgca gctgccgggg gtcatgggca ccgcagcggg
    22441 cctcggccag ccacggcccc tccaggtgca ccccggcgag ctcgtcgtcg gccacgagct
    22501 cggccagcgc gtcgacggcc cggagcagtt cagcgggtgc tgccgcgacg aggctcgcca
    22561 ccatggtcgt gctgccatgc gcccggtgga accgtacggc gcgcatcgcc tcctcggggt
    22621 cgccgtcggg aaaagcggca ccggcaccgc cgtggacgtg catgtcgacg aagccgggga
    22681 cgagtacggc gtcgcccagg ttgacgtccg gtgtgcgcgg cgacggtccg gcaccgaccg
    22741 cgctgatgcg ctcgcccgcc acctccaccc accccggtcc gaccaggccg tcggtgacga
    22801 cggtgtcggc cgcgagcagc agtgtcgcgg tcacagcgtc tccgtgatct tgctcaggcc
    22861 gcgcggcgcg tcggggtcgc cgccacggtc gacggcgacg tgcagggcga gcagctggaa
    22921 cgggatgatc tccagcagcg gggagagcgc ctcgggcaca ccggccggga gcgagaaccc
    22981 gaccgccacc cgcgccaccg cgtccgggtg ccccacgcag cacacgtcgg ccctgcgctc
    23041 ggcgagccgg ggcaacacat gctccatcgc cgccccaccg acgccctcgg ccaccacggc
    23101 gaggaccggc acctgggggt cgacgagcgc gagggggccg tgcagcaggt cggcgcccga
    23161 gaacgcctgc gccgagacgt aggcggtctc catcagcttc agcgcggcct cccgcgccgt
    23221 ggggtaggag tatccgcgtg cggtggtgat catccgctgg gcgaaccggt agcgctgcgc
    23281 gatcgcggcg atctctgcgg agcgggccag cagtgcgtca ccgaggtcgg gcagggccgc
    23341 tgcgggcccg gctccgccgt cccggccgtc gcgcacctgc tccagcagca ggtacagcgc
    23401 cagtagctgc gcggtgtagg acttggtcgc ggcgaccgac cgttcggccc cggccagtac
    23461 gtcgacgtgg gcgtgggcgg ctcgggcgag tgccgagtcg gcgttgttgg tcacggcgac
    23521 cgtcagcgcg ccctgggcgc gggcgacctc gatcgagcgg acgaggtcgg gcgagccgcc
    23581 ggactggctg atgccgatca tcaggacgtc gcgcaggtcg ggccgcgccc cgtacgccgt
    23641 catcgtcgac ggcgagacga gcccggcggg gagcccgagc cggatctccg cgaggtactt
    23701 ggcgtagagc gcggcgtggt cgctggtgcc acgggccacg aacaggacgg tgcgcggtgc
    23761 ccgactgcgg atcaacgcgg ccgcggcgtc gagcccggcg gcatgcccgt cgacgagcag
    23821 ccggcgccag gcggcgggct gctccgcgat ctccgccgcc atcaaccggc ccggccgctg
    23881 tgctgctgcc acggtcgtca tgcccgcggc tcgcggccgg tccttgcgag ttcgggtacg
    23941 gccccctcct gcgcagggtg gctgccctgc tcggggtcgg cgagcgccgc cgcgagccgg
    24001 acagcgccgg tgaccggtgc ctcggtgagc acacggacct cgacgtcggg gagccggtcg
    24061 cgcaccgccc gctgcagccg cggctggtgc accgcgacgc cgccaccgag caccacgggg
    24121 ccgtggccgt cgagccggtc gagcacccgc gtggccaggg cggccagcgc gtcggcggcc
    24181 acctcgagga tcgctgtggc ggcggcgtca ccgcgatcgg cgagttcggc gacggcgccg
    24241 gcccgctccg cccagtaccg ccggtcctgc gtgtcgtaga cgtggtcgat cagctgctcg
    24301 cgggtcgtga ggccacagtg ctccagcagt gcggcggtga gcgtgtcgac cgggaggccg
    24361 gcgtccatcc ggtccagcgc gtgtcgcacc gcgtcgagcg cgatgcggta tccgctgccc
    24421 tcgtcaccga gaaggtgccc ccagccgccg gcccgggcct ccgtgccgtc gctgcgggac
    24481 ccccaggcca ccgaccctgt ccccgagatc agggcgacgc cgtcgtcgag ccccgcggcg
    24541 gcgagcacga gccgggcgtc gtgcacgacg agcaccctgg cgccgggcac ccggtcggcg
    24601 aggaggcgtt ccagcgaacc caccgacgcg gtcccgtcgg cgccggcggc gcccgcgcag
    24661 acggcgtcga caccggcggg gtcgaggtcg gcgagcagcg cgtcgagctg ccggccagct
    24721 tcggcgacgc cgaccgcggc gatgttcgcg ctcccggcga cggcttcggc cacccggacc
    24781 ccgtcggcgg agcggacacc gtgggtcttg gagccgccga tgtcaatgcc gatgacgctc
    24841 cgcaactgca cccctacctg gctagatgtt tggtctagac gacttgcctc agattgtcag
    24901 acggtgtcca tgggagcaag cgtgcatgct gggcgcgtgg agggctgaca tcgctgctcc
    24961 gcagtgagtg aggcgaacga cg
//
LOCUS       DASRSC010000039         4057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_831333,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000039 DASRSC010000000
VERSION     DASRSC010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831333"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..148
                     /locus_tag="VFE39_01915"
     CDS             <1..148
                     /locus_tag="VFE39_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HET6257383.1"
                     /translation="HHYYIAVHALDVESLGIPQDATPAFLGFNLFSHTLARAVIVATY
                     ENQG"
     gene            complement(197..589)
                     /locus_tag="VFE39_01920"
     CDS             complement(197..589)
                     /locus_tag="VFE39_01920"
                     /inference="COORDINATES: protein motif:HMM:NF022963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3224 domain-containing protein"
                     /protein_id="HET6257384.1"
                     /translation="MTSTTGRFDLTRWDEEVIDDAAGAKLVRVSNSKTFEGGISGTSD
                     AELLQALAQEGSAAYVGIERVRGEIDGRKGTFVLRHSASGSVSGGDFRVDVVPDSATG
                     DLTGLRGALSIVKSPDGEHTYTFDYDLP"
     gene            complement(586..1560)
                     /locus_tag="VFE39_01925"
     CDS             complement(586..1560)
                     /locus_tag="VFE39_01925"
                     /inference="COORDINATES: protein motif:HMM:NF022517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HET6257385.1"
                     /translation="MVRNVHERLVPAPLTEVGRLLDRLGGPEDVLWPSPAWAPMVLDG
                     PLAVGVVGGHGPIRYRVTAYEPGRRVEFAFDPRTGLHGTHEFTAEAADPDSTMLRHVL
                     AGRLSGVARLMWPLAIRWLHDAVLEELLDNAGRAVGAPPARPAQWSPWVRLMRRFEAA
                     RARAVPVPATPLLVDALPRVDWTDAYAVPRRPGTPTDPQAWADAAFRTPPLWVAVVVG
                     VREALVGLVGIARGGSSSFDTVRRRADEVLLGTDERHLDFRASVLVEPDRVVLSTVVQ
                     VHNARGRAYSALVRRVHPVVVRAMLARAARLLSRSSNPSAATSTNIGV"
     gene            1612..2184
                     /locus_tag="VFE39_01930"
     CDS             1612..2184
                     /locus_tag="VFE39_01930"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6257386.1"
                     /translation="MAERKRLSAQDWAAAALDALSEGGVAAVSVEPIAARLGTTKGSF
                     YWHFSGRDALLEAALLRWEETHTGEVIALVEAEPDMRRRLRLLLTVALGGDAGHPGDR
                     VELALQPTAGHPLVAPVLARVTRRRLDYITGLFTALGFPAEQARHRSLLVYTAYLGHA
                     QLAHATPDATPRGPELPGYVDAVIATLTSA"
     gene            complement(2171..2935)
                     /locus_tag="VFE39_01935"
     CDS             complement(2171..2935)
                     /locus_tag="VFE39_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET6257387.1"
                     /translation="MIERDDHGGGDDRVAVLRLAHGPVSAMDLELCQAIGEQFRALAD
                     DPARAVVVTGTGRTFSAGADLKRYLHEGAPYVERFLPALSEAFRAVFELSKPVVAAVN
                     GHAIAGGCVLAACADVRLMAEGGGRIGVPEGRVGPPFPRIAIEVLRHAVGDVTARRLM
                     VGALTYPPAEALAMALVDRVLPPEELLPQAVATARAMATEVPSDTFALTKAQLNRDAL
                     ERTARYADEDAAVTGLWSRRAEDGWTARYLESLTQR"
     gene            3015..>4057
                     /locus_tag="VFE39_01940"
     CDS             3015..>4057
                     /locus_tag="VFE39_01940"
                     /EC_number="1.1.1.306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050607 - mycothiol-dependent formaldehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010127 - mycothiol-dependent
                     detoxification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)mycothiol dehydrogenase"
                     /protein_id="HET6257388.1"
                     /translation="MPQQVRGVVAHSKGKPVSLETITVPDPGPGEAVVQVQACGVCHT
                     DLHYREGGINDEFPFLLGHEAAGIVESVGDGVTEVAPGDFVILNWRAVDGTCRACRRG
                     EPWYCFSTHNAAQKMTLESGQELTPALGIGAFAEKTLVHSGQCTKVNPAVKPEVAGLL
                     GCGVMAGIGAAINTGKVGRGDSIAVIGCGGVGNAAIAGARLAGATTIIAIDVDDRKLQ
                     WAKEFGATHTINSREQDPVEGVQALTDGNGANVVVDAVGRPETFKQAFYARDLAGTVV
                     LVGVPTPDLTIDLPLIDVFGRGGATKSSWYGDCLPSRDFPMLVDLHLQGRLPLDKFVS
                     ETIGLEDVEAAFE"
BASE COUNT          608 a         1498 c         1423 g          528 t
ORIGIN      
        1 gcaccactac tacatcgcgg tgcacgcctt ggacgtcgag agcctgggga taccgcagga
       61 cgcgacgccg gcgttcctcg gcttcaacct gttcagccac acccttgccc gcgccgtgat
      121 cgtcgccacc tacgagaacc agggctgacg cgcccggtcc agtacgcggc gcgcggatct
      181 cagggcaggt cggatctcag ggcaggtcgt agtcgaacgt gtaggtgtgc tcgccatcgg
      241 gcgacttgac gatcgagagc gcgccgcgca ggccggtgag atcgccggtg gcggagtcgg
      301 gcaccacgtc cacacggaag tcgccgccgc tcacgctccc ggacgcggag tggcgcagca
      361 cgaaggtgcc cttccggccg tcgatctcgc cgcgcacccg ctcgatgccg acgtaggccg
      421 ccgagccctc ctgcgcaagg gcctgcagca actccgcgtc gctcgtgccc gagatgccac
      481 cctcgaacgt cttcgagttg ctcacccgca cgagcttcgc gcccgcggcg tcgtcgatga
      541 cctcctcgtc ccagcgcgtg aggtcgaacc ggcccgtcgt gcttgtcata ccccgatgtt
      601 cgtggatgtg gccgccgacg gattggacga acgcgacagg agccgggcgg cccgggcgag
      661 catcgcgcgc acgaccaccg ggtgcacgcg gcggacgagc gcggagtacg cccggccgcg
      721 ggcgttgtgg acctgcacga cggtgctcag caccacccgg tccggctcga cgagcacgga
      781 cgcgcggaag tcgaggtggc gctcgtcggt gccgaggagc acctcgtcgg cccgtcgccg
      841 gacggtgtcg aacgacgacg atccgccgcg ggcgatgccc acgaggccga cgagcgcctc
      901 ccgcacgccg accacgaccg ccacccacag cggcggggta cggaacgcgg catcggccca
      961 ggcctgcggg tcggtggggg tgcccggcct ccgtggcacg gcgtaggcgt ccgtccagtc
     1021 gacccgcggg agggcgtcca cgagcagggg cgttgcgggc accggaacgg ctcgggcgcg
     1081 ggccgcctcg aacctgcgca tgagccggac ccacggcgac cactgagctg ggcgcgccgg
     1141 cggtgcgccg accgcacgcc cggcgttgtc gaggagctcc tcgagcactg cgtcgtgcaa
     1201 ccagcggata gcgagcggcc acatcagcct ggcgacaccg ctcagacggc ccgccaggac
     1261 gtggcgcagc atcgtgctgt ccggatccgc ggcttcggcg gtgaattcgt gcgtgccgtg
     1321 caggcccgtc cgcgggtcga acgcgaactc cacccgcctg cccggctcgt acgccgtgac
     1381 gcggtaccgg atcgggccgt ggccgcccac caccccgact gcgaggggac cgtcgagcac
     1441 catcggcgcc caggccggcg acggccacag cacgtcctcc ggaccgccga gccggtcgag
     1501 cagccggccg acctcggtga gcggtgcggg caccagccgt tcatggacgt tgcggaccat
     1561 ccccgacctc cgtacgctac cgtatggtga accgtacggt agcgtatgga cgtggcggag
     1621 cggaagcggt tgtccgcgca ggactgggcg gcggccgccc tcgacgccct ctcggagggc
     1681 ggagtggccg cggtctcggt cgagcccatc gccgcgcggc tgggcaccac gaaaggcagc
     1741 ttctactggc acttcagcgg ccgcgacgcg ctgctggaag cggcgctgct gcgctgggag
     1801 gagacccaca ccggcgaggt cattgcgctc gtcgaggccg agcctgacat gcggcgcagg
     1861 ctccgcctcc tgctcaccgt ggcgctcggg ggcgacgccg gccaccccgg cgaccgcgtg
     1921 gaactcgccc tccagcccac ggcaggccat ccgctggtgg cacccgtgct cgcccgcgtc
     1981 acgcgccggc ggctcgacta catcaccggc ctgttcaccg cgctcggctt ccccgccgag
     2041 caggcccggc accggagcct gctggtctac acggcctacc tcgggcatgc gcagctcgcg
     2101 cacgccaccc ccgacgccac accccggggc cccgagctgc ccggctacgt ggacgccgtg
     2161 atcgccactc tcacctctgc gtgagggact ccaggtaccg cgccgtccag ccgtcctcgg
     2221 cgcgccggct ccacagcccc gtcaccgccg cgtcctcgtc ggcataccgg gccgtgcgct
     2281 ccagggcgtc gcggttcagc tgggccttcg tcagggcgaa cgtgtcggac ggcacctcgg
     2341 tggccatcgc cctcgccgtg gcgactgcct gcgggagcag ctcctccggc ggcagcaccc
     2401 ggtcgacgag cgccatggcc agcgcctcgg ccgggggata ggtcagggca ccgaccatca
     2461 gtcggcgtgc ggtcacgtcg cccacggcgt gccgcagcac ctcgatcgcg atccgcggga
     2521 acggcgggcc tacccgaccc tccggaacgc cgatccgccc accgccctcg gccatcagcc
     2581 tgacgtccgc acatgcggcc agcacgcagc ccccggcgat cgcgtgcccg ttgaccgcgg
     2641 cgaccacggg cttcgacagc tcgaacaccg cccggaacgc ctccgacaac gccggcagga
     2701 accgctcgac gtacggcgcc ccctcatgga ggtagcgctt cagatcggca cctgccgaaa
     2761 aggtacggcc cgttccggtg acgacgacgg cacgggcagg gtcgtcggcg agggcgcgga
     2821 actgctcgcc gatcgcctgg cacagctcga gatccatggc gctgaccggc ccgtgcgcaa
     2881 gccgcagcac cgcgacgcgg tcgtcgccac cgccatggtc atcccgttcg atcatgagcc
     2941 ggaacctacc cggcgcgtca tctcatggca ccctgcgcag gggagagctc agaagagaga
     3001 gttggggaag agtcatgccg caacaggtcc gcggggtcgt cgcgcacagc aagggcaagc
     3061 cggtctcgct ggagacgatc accgtgccgg atcccggacc cggcgaggcg gtcgtgcagg
     3121 tacaggcctg cggggtctgc cacaccgacc tgcactaccg cgagggcgga atcaacgacg
     3181 agttcccgtt cctgctcggc cacgaggcgg ccgggatcgt ggagtcggtg ggtgatggcg
     3241 tcaccgaggt ggcgcccggc gacttcgtga tcctcaactg gcgggccgtc gacggcacct
     3301 gccgggcctg ccgccgtggg gagccctggt actgcttctc cacgcacaac gccgcgcaga
     3361 agatgacgct ggagtcgggc caggagctga ccccggcgct cggcatcggc gcgttcgccg
     3421 agaagacact ggtgcactcc gggcagtgca cgaaggtgaa cccagcggtc aagccggagg
     3481 tggccggcct gctcggctgc ggcgtgatgg ccggcatcgg tgcggcgatc aacaccggca
     3541 aggtcgggcg cggtgactcc atcgccgtga tcggctgcgg cggcgtgggc aacgccgcga
     3601 tcgccggcgc aaggctcgcc ggcgccacca cgatcatcgc catcgacgtg gacgaccgga
     3661 agctgcagtg ggcgaaggag ttcggcgcca cccacacgat caactcccgc gagcaggacc
     3721 cggtcgaggg cgtgcaggcg ctgaccgacg gcaacggcgc caacgtcgtg gtcgacgcgg
     3781 tcggcaggcc cgagacgttc aagcaggcgt tctacgcccg ggacctggcc ggcaccgtcg
     3841 tgctcgtcgg cgtgccgacg ccggacttga cgatcgacct gccgctcatc gacgtcttcg
     3901 gccgcggtgg cgccaccaaa tcgtcctggt acggcgactg cctgcccagc cgcgacttcc
     3961 cgatgctcgt cgacctgcac ctgcagggca ggctgccgct cgacaagttc gtcagcgaga
     4021 cgatcgggct cgaggacgtc gaggccgcgt tcgagaa
//
LOCUS       DASRSC010000040         7641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4597798,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000040 DASRSC010000000
VERSION     DASRSC010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7641
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4597798"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFE39_01945"
     CDS             <1..213
                     /locus_tag="VFE39_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET6257389.1"
                     /translation="VESVLETPEVLGVERVGPEGVTLRMTVKVKPGQQFAVQRALNAA
                     ITNALDHAGVPRPGVVSPPVARPSVP"
     gene            247..630
                     /locus_tag="VFE39_01950"
     CDS             247..630
                     /locus_tag="VFE39_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HET6257390.1"
                     /translation="MSDPQNFYAEAGGAPVFDKIVSRFYEQVSEDEVLRPIYPEEDLG
                     PAEVRLRMFLEQYWGGPRTYSEQRGHPRLRMRHAPFKVGPIERDAWLRCMRVAVDEAG
                     LDDEHRAQLWQYLTYAAASMVNSDF"
     gene            709..2274
                     /locus_tag="VFE39_01955"
     CDS             709..2274
                     /locus_tag="VFE39_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HET6257391.1"
                     /translation="MYQIYVRSFSDGDGDGIGDLQGIRSRLGYLELLGADALWLTPFF
                     TSPMVDHGYDVADPRSVDPVFGDLDTFDGLIADAHEHGLRVAIDLVPNHTSSEHEWFR
                     AALDSTPGSPERARYHFRDGRGPDGAEPPNNWTSIVGGPAWTRVQDPDSDGEWNPGEW
                     YLHLLTPDQPDVNWTNPEVWADLEKTLRFWLDHGVDGFRIGIADGMSKPEGLPDIEER
                     PEGAGATAIRLLAPVDDDPRFDDAGVHDVHRMIRAVLDHYPGAMVVGEVRIGDNERFA
                     RYLWPDELHLAFNFRLVQAPFDANDVRTAIEQSLAAVEKVGAPPTWTLSNHDVSRPVT
                     RYGGGPAGRARARAMVLVELALPGVVYLYNGEELGLPDGELPDAVLQDPQWERSGHTE
                     RGRDGCRVPVPWEGGAPGYGFTSGQPWLPMPPEYGRLTVAGQLEDTTSTLSLIRRAVE
                     LRKTHPGFDGRSVEWFGAPVGCLAFRRAGSTLVCALNASSDLVPLPPGDVLLASGPLE
                     GDLLPPDTAAWLA"
     gene            complement(2316..2963)
                     /locus_tag="VFE39_01960"
     CDS             complement(2316..2963)
                     /locus_tag="VFE39_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257392.1"
                     /translation="MTSLTVRDAAERGDLGAFVARVVRLDQRATVRLRASGGRVTAWA
                     STPFDVLATRSVHGLLEPGDVTVPATGLLTALAVERAETVDPGAGGLWQGELPPDEGW
                     APVDDVPAGELDALTERGLVLARENAGPLGPPASLLDQIVLTVTATSGPAIKIPLRCL
                     FALSGMGFLGDTGAGGAGGAVGAVRVSATGTWLRLDARYGAVVRRRVTALPLLLA"
     gene            complement(2960..3376)
                     /locus_tag="VFE39_01965"
     CDS             complement(2960..3376)
                     /locus_tag="VFE39_01965"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HET6257393.1"
                     /translation="MPRFVAQVPLRWTDQDSYRHVNHARAVTLLEEARIGLFFDTAAA
                     EGIQGFTGGLLVAGLNVDYRRQIPYRSHPLRVGMWVDEVRAASFRICYEMHDGPAAAD
                     PVAVVAWTRMAIFDLDAQRPRRLTADERAFLGRWAA"
     gene            complement(3449..7624)
                     /locus_tag="VFE39_01970"
     CDS             complement(3449..7624)
                     /locus_tag="VFE39_01970"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HET6257394.1"
                     /translation="MTLSARPAVSGDSRPAEVADLLRWLADEHFTFLGYRRYAVPEGA
                     ADLRAEPGSGLGVLRRENGITATFAPQLDAAERPRELLVLTRANTLSPLRPVHPYYLA
                     VRTVDATGRLVGEHRFLGTLTVPALYENVLDIPVLERRVRDAIHRAGFPLESYSGQQM
                     LEVISALPREELFSSSEQRLHDTAVGVLAVAGRRAVRLFLRPDPYRRFISCLVYLPRD
                     RYTTASRLRMADVLQRRLGGRSVQHTARVSESNLALVHFTVHTDAGAPAFGDVDVPEL
                     QDELTEAVRIWDDRLLSQPQAAGVAALLPGVPEAYKAAVAPQHAVEDLRRVAALPGPG
                     AFDVRLYSAPEPASRSDERRFSLYLAGAPATLTAVLPLLQQLGVDVLDERPSEFVRAD
                     GLRCWLYDFGLRMDDATRAVLAERTDDAIERGFCSAFSAAWRGDAETDRFSALVLRAG
                     LPWREVAVLRAYAHYARQLGAPYGAQYMAETLLAHHAVARALVALFRVRFDPAIEERE
                     REILAALTEVRGLIDAVTGLDADRILRGYLAMITATLRTNWFRDRPFFSFKIDPSAVP
                     DMPAPRPKFEIFVYSPRVEGVHLRFGPVARGGLRYSDRPQDYRTEILGLVKAQAVKNA
                     VIVPVGAKGGFIVRRGDAGPDELEYCYRTFISGLLDVTDNLVNGTTVPPPDVVRHDGD
                     DSYLVVAADKGTARFSDTANEVAASYGFWLGDAFASGGSVGYDHKAMGITAKGAWESV
                     KRHFRELGIDTQRQEFTVVGIGDMSGDVFGNGMLLSPHIRLLAAFDHRHVFVDPTPDP
                     ARSFAERQRMFALPRSSWDDYDRSVISPGGGVWPRTAKSVPIGPEIRAALGIGEGPET
                     LSPPELIAAILRAPADLLWNGGIGTYVKASSESHADAGDKANDAVRADGAELRVKVVG
                     EGGNLGLTQRGRIEFARRGGKINTDAIDNSAGVDCSDHEVNIKVLLDRLVVAGELDRA
                     GRDALLVEMTDDVADLVLDDNRDQNAVLGIARAHAPAMVNVHGRLTTDLVARRGLDRK
                     LEVLPDEAGFAALEDAGGGLASPELATLLAHTKLDLTAQVLATDLPESAAFAARLPGY
                     FPRPLRDRFPGAVAGHPLRREIVITQLVNEMVDGAGMTYAFRLGEELSAAPTDAVRAY
                     AVTTAVFDLPGLWEELRAPQIPTAVSDEIVLESRRLLDRASRWFLTNRPQPLAVGAET
                     ARFAAAVRELRAELPRLLRGRELAAVEMRAAQLRSSGVAADCAMRSAALIYGYGLLDV
                     VELTELAERDREPREPGEVAALYYGLSEHLGIDLALTSVSALERGDRWHALARLALRD
                     DLYGSLRAITLDALREDAPGTPVETAIAQWEKANASRLLRARAALHEVGTAGQLDLAT
                     LSVVSRQLRGLAR"
BASE COUNT         1144 a         2778 c         2698 g         1021 t
ORIGIN      
        1 gtcgagtccg tgctggagac gccggaggtg ctgggcgtcg aacgggtcgg accggagggc
       61 gtgacgctgc ggatgacggt gaaggtcaag ccgggccagc agttcgccgt gcagcgcgcc
      121 ctgaacgcgg cgatcaccaa cgcgctcgac cacgctggtg tgccgcgccc cggtgtggta
      181 tccccacccg tggcccgtcc gtcggtgccc tgaaccgcac ccgcccgggc ggaggatgat
      241 gaacctgtga gcgatcctca gaacttctac gccgaagccg gcggagcacc cgtcttcgac
      301 aagatcgtgt ctcgtttcta cgagcaggtc agcgaggacg aggtgctccg cccgatctac
      361 ccggaggagg acctcggtcc ggccgaggtc cggctgcgga tgttcctcga gcagtattgg
      421 ggcggcccac gcacctactc cgagcagcgc gggcacccgc ggctgcggat gcggcacgcg
      481 ccgttcaagg tcggcccgat cgagcgcgac gcgtggctgc gctgcatgcg ggtcgccgtc
      541 gacgaggccg ggctggacga cgagcaccgc gcgcagctgt ggcagtacct cacctacgcc
      601 gccgctagca tggtcaacag cgacttctga agccgaacgg gcggttcgcg ctcatactgc
      661 tccggatttg gcaagatctc ccgggtgcag tggtggcggg acgccgtgat gtaccagatc
      721 tacgtccggt cgttctccga cggcgacggg gacggcatcg gtgatctgca gggcatccgg
      781 tcccggctgg gctacctgga actgctgggc gccgacgccc tgtggctgac gccgttcttc
      841 acctcaccca tggtcgacca cggctatgac gtggccgacc cgcgcagcgt cgatccggtg
      901 ttcggcgact tggacacgtt cgacgggctc attgccgacg cgcacgagca cggcctgcgg
      961 gtcgcgatcg acctggtgcc caaccacacg tcgagcgagc acgaatggtt ccgggcagcg
     1021 ctggactcca ccccgggcag ccccgagcgg gcgcgctacc acttccgtga tggccgcggg
     1081 cccgacggtg ccgaaccgcc gaacaactgg acgagcatcg tcggcggccc ggcctggaca
     1141 cgcgtacagg atcccgactc cgacggggaa tggaaccccg gcgagtggta cctgcacctc
     1201 ctcacgcccg accagcccga cgtcaactgg accaaccccg aggtgtgggc cgacctggag
     1261 aagacgctgc ggttctggct ggaccacggc gtcgacggct tccgcatcgg tatcgccgac
     1321 ggcatgagca agccggaagg gctgcccgac atcgaggagc gcccggaggg cgcaggcgcc
     1381 accgccatcc ggctgctggc tcccgtcgac gacgacccgc gcttcgacga tgccggcgtg
     1441 cacgacgtcc atcggatgat ccgcgccgtg ctcgaccact acccaggagc gatggtggtg
     1501 ggcgaggtca ggatcggtga caatgagcgg ttcgcccgct acctgtggcc cgacgagttg
     1561 cacctcgcct tcaacttccg gctcgtgcag gccccgttcg acgccaacga tgtacggacg
     1621 gcgatcgagc agtcgctcgc cgctgtggag aaggtgggcg ccccaccgac gtggacgctg
     1681 tccaaccacg acgtgtcccg gccggtcacc cgctacggcg gcggcccggc cgggcgggcc
     1741 agggcgcggg cgatggtatt ggtcgagctc gccctgcccg gcgtggtgta cctctacaac
     1801 ggcgaggagc tcggcctgcc cgacggcgag ctgcctgacg cggtgttgca ggacccgcag
     1861 tgggaacgct cgggtcacac cgaacgcggg cgtgacggct gccgcgtgcc ggtgccgtgg
     1921 gaggggggcg caccgggcta cgggttcacg agcggccaac cgtggctgcc gatgccaccc
     1981 gagtacggtc ggctcaccgt ggccggccag ctcgaggaca ccacgtcgac gctgtccctg
     2041 atccgccggg ccgtggagct gcgcaagacc caccccggat tcgacggccg gagcgtcgag
     2101 tggttcggag ctccggtggg atgcctggcg ttccgccgag ccggatccac cctggtgtgc
     2161 gccctcaacg cctcttccga tctcgtcccg ctcccgccgg gcgacgtcct tctggccagc
     2221 ggtccgctcg agggcgacct gctgcccccg gacacggcgg cctggctcgc ctgacgctcg
     2281 gtggcggcca tcggagcccg tgacggcggc gcccgtcagg cgaggagcag cggtagcgcc
     2341 gtgaccctgc gccggaccac ggcgccgtag cgggcgtcga gccgcagcca ggtgccggtg
     2401 gcggacacgc gcaccgcgcc gaccgcaccg cccgcaccgc ccgcaccggt gtcaccgagg
     2461 aaccccatcc cggacagcgc gaacaggcag cgcagcggaa tcttgatcgc cggccccgac
     2521 gtggccgtca cggtgagcac gatctggtcc agcaacgacg caggcggacc gagcggcccc
     2581 gcattctccc tggccaggac gagcccccgc tcggtgagag cgtcaagctc cccggccggc
     2641 acgtcgtcca ccggagccca gccctcgtcc ggcggcagct cgccctgcca gagtccaccg
     2701 gcgccagggt cgaccgtctc ggcgcgttcc acggcgagcg ccgtgagcag ccccgtcgcc
     2761 ggcacggtga cgtcaccggg ctccagcagg ccgtgcacag agcgcgtggc cagcacgtcg
     2821 aacggggtcg acgcccacgc ggtgactctg ccgccgctcg cccgcagccg cacggtcgcc
     2881 ctctggtcca gccgcaccac ccgcgccacg aaggcgccga ggtcgccgcg ctcggcagcg
     2941 tctcgcacgg tcagcgatgt catgccgccc acctgccgag gaacgcacgc tcgtcagcgg
     3001 tcagccggcg tggccgctgc gcgtcgaggt cgaagatggc catccgggtc caggccacga
     3061 cggcgacagg gtcggccgcc gccggaccgt cgtgcatctc gtaacagatc cggaacgacg
     3121 ccgcgcgcac ctcgtccacc cacatcccca cccgcagcgg atgcgaccgg tacgggatct
     3181 ggcgccgata gtccacgttc agaccggcga cgagcagacc accggtgaag ccctggatcc
     3241 cctccgccgc ggcggtgtcg aagaacaggc cgatccgggc ctcctccagc aacgtcaccg
     3301 cgcgggcgtg gttgacatgc cggtaggagt cctggtcggt ccagcgcagg gggacctgcg
     3361 caacgaagcg aggcacgtcg cccaccctct caggatcgtg ccgtcggccg gacagggtgt
     3421 ccggccccgg gtcgggtcga tgcgcgggtc agcgcgcgag cccacgcagc tgccgcgaga
     3481 ccaccgacag ggtcgcgagg tcgagctgcc ccgcggtgcc gacctcgtgc agcgcggccc
     3541 gggctcgcag caggcgcgag gcgttcgcct tctcccactg cgcgatggcc gtctccaccg
     3601 gcgtgcccgg tgcgtcctcg cgcagcgcgt cgagggtgat ggcccgcagc gacccgtaca
     3661 ggtcgtcgcg cagtgccagc ctggccaggg cgtgccagcg gtcgccgcgt tccagtgcac
     3721 tcaccgaggt gagcgcgagg tcgatgccca gatgctcgga caggccgtag tacagcgcgg
     3781 ccacctcgcc gggttctcgc ggctcgcggt cgcgttcggc cagttccgtc agctcgacga
     3841 cgtcgagcaa cccgtagccg tatatcagcg ccgccgagcg catcgcacag tccgcagcga
     3901 ccccggacga ccgcagctgc gccgcccgca tctcgacggc ggcgagctcc cggccgcgca
     3961 gcagccgcgg cagctcggcc cgcagctccc ggacggcagc ggcgaagcgc gctgtctccg
     4021 cgccgacggc cagcggctgc ggccggttgg tgaggaacca gcgcgacgcg cggtcgagca
     4081 ggcgacgcga ctccagcacg atctcatcgg acaccgcggt cgggatctgc ggcgcgcgca
     4141 gctcctccca caggccgggc aggtcgaaca ccgcggtggt cacggcgtag gcgcgcaccg
     4201 catcggtggg cgcggcggag agctcctcgc cgagccggaa tgcataggtc atcccagcac
     4261 cgtcgaccat ctcgttgacc agctgcgtga tcacgatctc cctgcgcagc gggtggccgg
     4321 cgaccgctcc cgggaagcgg tcacgcagcg gacgcgggaa ataccccggc agccgggcgg
     4381 cgaaggccgc actctcgggc aggtcggtgg ccagcacctg cgccgtgagg tcgagcttcg
     4441 tgtgtgcgag caacgtggcg agctcggggc tggcgagtcc cccgccggcg tcctccaacg
     4501 cggcgaagcc tgcctcgtcg ggcagcactt ccagcttccg gtccagcccg cggcgggcga
     4561 cgaggtccgt ggtgagcctg ccgtgcacgt tcaccatcgc cggagcgtgc gcccgggcga
     4621 tgcccagcac cgcgttctgg tcacggttgt cgtccagcac gagatcggca acgtcatcgg
     4681 tcatctcgac aagcagcgcg tcgcggcctg cgcggtcgag ctcgcccgcg accaccagcc
     4741 ggtcgagcag gaccttgatg ttgacctcgt ggtcggagca gtccacaccc gccgagttgt
     4801 cgatcgcgtc agtgttgatc ttcccgcccc gacgcgcgaa ctcgatgcgc ccccgctggg
     4861 tcagtccgag gttgccgccc tcgcccacga ccttcacccg cagctccgcg ccgtcggccc
     4921 gcacggcgtc gttcgccttg tccccggcgt cggcgtgcga ctcgctcgac gccttgacgt
     4981 acgtaccgat cccgccgttc cacagcaggt cggccggagc gcgcaggatc gccgcgatca
     5041 gctcgggcgg gctcagcgtc tccgggccct ccccgatccc cagcgcggcc cggatctccg
     5101 gcccgatcgg cacggacttt gcggtgcgcg gccacacgcc accacccggg ctgatcaccg
     5161 agcggtcgta gtcgtcccac gacgagcggg gcagcgcgaa catccgctgc cgctcggcga
     5221 agctgcgggc cggatccggt gtcgggtcga cgaagacgtg ccggtggtcg aacgccgcga
     5281 gcagccggat gtgtggcgac agcagcatcc cgttgccgaa cacgtcgccg gacatgtcgc
     5341 cgataccgac gaccgtgaac tcctggcgct gtgtgtcgat gcccagctcg cggaagtggc
     5401 gcttgacgct ttcccacgcg ccctttgccg tgatgcccat ggccttgtgg tcgtagccga
     5461 cggagccgcc ggaggcgaac gcgtcaccga gccagaagcc gtaggacgcg gccacctcgt
     5521 tggcggtgtc ggagaaccgg gccgtgccct tgtccgccgc gaccaccaga taggagtcgt
     5581 ccccgtcatg acggacgacg tcgggtggtg gcaccgtcgt gccgttcacg aggttgtcgg
     5641 tgacgtccag cagacccgag atgaacgtcc ggtagcagta ctcgagctcg tcggggcccg
     5701 cgtcgccgcg gcgaacgatg aagcccccct tggcgccgac gggcacgatc accgcattct
     5761 tgacggcctg cgccttgacc agcccgagga tctccgtgcg gtagtcctgc ggccggtcgg
     5821 agtagcgcag gccaccccgc gcgaccggcc cgaaccgcag gtgcacaccc tcgacccggg
     5881 gcgagtacac gaagatctcg aacttcggcc gcggcgccgg catgtcgggc accgcggaag
     5941 gatcgatctt gaacgagaag aacggccgat cgcggaacca gttcgtgcgc agcgtcgcgg
     6001 tgatcatcgc caggtacccg cggaggatgc ggtcggcatc gagccccgtc acggcgtcga
     6061 tcaggccccg cacctcggtc agcgccgcga ggatctcccg ctcgcgctcc tcgatagccg
     6121 gatcgaaccg gacccggaac aacgcgacga gtgcgcgcgc gactgcatga tgcgcaagca
     6181 gcgtctcggc catgtactgc gcgccgtagg gggcgccgag ctgccgggcg tagtgggcgt
     6241 acgcgcgcag caccgcgacc tcccgccacg gcaacccggc ccgcagcacg agtgcggaga
     6301 acctgtcggt ctcggcgtca ccacgccacg cggcgctgaa cgccgagcag aagccgcgct
     6361 cgatggcatc gtcggtgcgc tcggccagca cggcccgggt ggcgtcgtcc atccgcaggc
     6421 cgaagtcgta aagccagcag cggagcccgt cggcacgcac gaactccgac ggtcgctcgt
     6481 cgagcacatc cacgccgagc tgctggagca acggaagtac cgcggtcaat gtggcgggcg
     6541 ccccggcgag gtagaggctg aaccgccgct cgtcggacct cgatgccggt tcgggcgccg
     6601 agtagagccg gacgtcgaac gccccgggac cgggcagcgc ggcgacccgg cgcaggtcct
     6661 cgacggcatg ctggggagcg acggccgcct tgtacgcctc cggcacaccg ggcagcagcg
     6721 cagccacgcc tgctgcctgc ggctggctca gcaaccggtc gtcccagatg cggacggcct
     6781 cggtcagctc gtcctgcagc tccggcacgt cgacgtcgcc gaacgccggc gcacccgcgt
     6841 cggtgtgcac cgtgaagtgc accaatgcga ggttcgactc cgagacgcgc gcggtgtgct
     6901 gcacgctgcg cccgccgagg cggcgctgca gcacgtcggc catccgcagg cgggacgccg
     6961 tggtgtaccg gtcgcggggc aggtacacca ggcacgagat gaaccggcgg tacgggtcgg
     7021 gacgcaggaa cagccgcacc gcgcgtcgcc cggccacggc gagcacaccc acggcggtgt
     7081 cgtgcaggcg ctgttcgctc gagctgaaca gctcctcccg gggcagcgcg gagatcacct
     7141 cgagcatctg ctgaccggag taggactcca gcgggaaccc ggcgcggtgg atcgcgtcgc
     7201 gcaccctgcg ctcgaggaca gggatgtcga ggacgttctc gtagagcgcg ggcacggtca
     7261 gcgtgccgag gaaccggtgc tcgccgacga gccggcccgt ggcgtcgacc gtccgcacgg
     7321 cgaggtagta cggatgcacg ggacgcagcg ggctgagcgt gttggcgcgg gtgagcacga
     7381 gcagctcgcg gggcctctcc gccgcgtcga gctgcggtgc gaacgtggcg gtgatgccgt
     7441 tctcgcggcg cagcacgccc aggccggaac cgggctcggc gcgcaggtcc gcagcccctt
     7501 cgggcaccgc gtagcggcgg tagccgagga acgtgaagtg ctcgtcggcc agccagcgca
     7561 gcaggtccgc gacctccgcc ggccggctgt caccggacac agcgggccgc gccgacagcg
     7621 tcacggccag ttccctggcc g
//
LOCUS       DASRSC010000041         9397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5743996,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000041 DASRSC010000000
VERSION     DASRSC010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5743996"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            624..2063
                     /locus_tag="VFE39_01975"
     CDS             624..2063
                     /locus_tag="VFE39_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_030063052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HET6257395.1"
                     /translation="MLRTRAPEVSLWEAVLPDEVLRLPEELARVDALLDDPVFFAPFA
                     PFFDPRLGRPSTPMEVYLRLMFLKFRYRLGYETLCREVADSITWRRFCRIPIDGRVPH
                     PTTLMKLTTRCGTTAVDGCNEALLAKAAQARLLRTTRLRADTTVVPADVAYPTDSGLL
                     AKAVRRIAAAGRRVQAAGGATRTRVRDRSRSAGARAREIGAKLRLRAAQTRDEAQAVV
                     KRITGELAGLAERAAAEAEALLGNARRALRRAQTKAAQLAAAGEQDPDAGRRRGRLHR
                     AVNDLTDLLAATRRIAAQTRQRLSGTTPEGATRRVSLHDGDARPIAKGRLGRPVEFGY
                     KGQVVDNDDGVVLDHTLERGNPADAPQLAPAVGRVIGRTGRRPRTVTADRGYGEAGVE
                     NDLHDLGVRTVVIPRKGRPGNARQAHEHRRAFRRTVKWRTGSEARISTLKRQHGWDRT
                     RLDHLDGARIWTGHGVLAHNLVKIAALAG"
     gene            2165..2665
                     /locus_tag="VFE39_01980"
     CDS             2165..2665
                     /locus_tag="VFE39_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6257396.1"
                     /translation="MRFRVVPAAYVLPLRRVGRTDEVLLQLRRGTGYMDGHWAAAAAG
                     HVEADESVHAAACREAEEELGIGIVTADLVPLTVMHRTHGNHRAIDERVDFFFSCRTW
                     TGDPRRVEQAKSADLRWCSLDDLPEPVVPHELFVLAGLRRGDLPAIVSFGFDAATSPD
                     ARAPRR"
     gene            complement(2675..4234)
                     /locus_tag="VFE39_01985"
     CDS             complement(2675..4234)
                     /locus_tag="VFE39_01985"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HET6257397.1"
                     /translation="MTTQLTDFTSALARWEAERPDAVALRFGDTARTWSELTRRVRQN
                     AAAQRAEGLLPGDRVAVLDLNHPSCLELTLACAQVGTANAVVNFRLAPPEIVYVINDA
                     AARILFVGPEFAAAVEQVRDKLPTVERVIRVGGADDEYEAWLAARQPDPEVYPADRED
                     CFVQLYTSGTTGFPKGAMLTHRSMLAHAENVATDFELGPDARVQVAMPMYHVGGTSYA
                     LLAISAGAQIMMLRLPDPVAILKVLESERITHTFLVPALMAAMAQVPGAADRDFSSLL
                     ALSYGASPMPLPVMRACLKLFPGVMHQVYGMTEASGVVSSLGPEDHDDPAVAHRLISA
                     GTSIHGVEIEIRDPATGDPVPTGEAGEIWVRTEQLMGGYWGKPDATAAAVTPDGWLRS
                     GDGGHIDADGYVYVTDRIKDMIISGGENIYPAEIERVLAEHPTVADVAVIGVPDERWG
                     EVAKAIVVAAPGVEFDGDALLAYCREQLAAFKCPKSVDVLDELPRNPTGKVLKKDLRA
                     PYWEGKERRVV"
     gene            complement(4349..4945)
                     /gene="rfbC"
                     /locus_tag="VFE39_01990"
     CDS             complement(4349..4945)
                     /gene="rfbC"
                     /locus_tag="VFE39_01990"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET6257398.1"
                     /translation="MQVHRTELAGVLLFEPKPHHDDRGWFSRTFESSAARSAGIDPGT
                     FVQDSQSRSRKGVIRGLHGRFGKGEAKFVRCSSGALFDVVIDARTDSPTFGRWAAFRL
                     DDERMLGLYIPRGFLHGFQALTETADICYRIDAEHDPTEDIAVRWDDPHLTIDWPLAP
                     TLISERDRQAGTWTELQSFLRGARKNSALTGSRGRGRA"
     gene            5304..6065
                     /locus_tag="VFE39_01995"
     CDS             5304..6065
                     /locus_tag="VFE39_01995"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6257399.1"
                     /translation="MSSPLQVPRMRATARRVKAGGERFLGGVLPGASARRLRERAFAD
                     DWAAANLDARQATGPLWVVLGDSVSQGIGATTREVGYVGVVHEMLRRRDAWRVVNLSR
                     AGAGVADVLARQLPELAALTAAEPAALVSCIIGAEDLARRTPGLDTSLRQVIAALPTG
                     SVIGTLSRGGRGGPAAALDAVIREEAARHQLRIAQLPSRAGDPGRVRDHTLLSDVGHA
                     AWATAVLKAIDGPPAEIASTTDPALPVVPAVPDTD"
     gene            6103..6993
                     /locus_tag="VFE39_02000"
     CDS             6103..6993
                     /locus_tag="VFE39_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6257400.1"
                     /translation="MVRELSKRFGSITAVDGVTFSVEPGAVTGFLGPNGSGKTTTLRM
                     ILGLVTPTSGGAHVNGVPFAELANPARVVGAVLEAQGFHPGRTARKHLLVCAAAIGVP
                     DAAVDHVLGSVGLAHAADRGVGGYSLGMRQRLALAVALLGDPQVLILDEPANGLDPEG
                     IAWLRAFLRGFAGQGRSVLVSSHLLAEVEQTADHLVVISRGRCVFQGSLAQLRGSRQT
                     RVLVQCPAPARLADALARTGLLEIDTLPDGRLGVAGTDPVHVGDVALAAGVAIHGLVE
                     EKIDLEQLFFQLTSGPWRAG"
     gene            6978..7808
                     /locus_tag="VFE39_02005"
     CDS             6978..7808
                     /locus_tag="VFE39_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257401.1"
                     /translation="MAGRLMGRLLRAEVRKTFSTKLWWALLVPVALLSIVINLFGGLF
                     AAAIPNKGAGLPLLLGSLAYALGLTGVFAACYGVVAMAAEFRHRTITTTYLTEPRRGR
                     VLLAKMATSAAVGALYAAVVALVGVLAGLAGQGGAAPAVDQLLAVSAIGMAVAALWSA
                     LGSALGTVISNQVSGLVGILVYLLLGELLISALLNSANSEAVPRLTAYLPGNAGDVAI
                     YDIPAHVLAGSDLGPTVVETLAGVTAPPPWWGALLVLAAWVAAVAAAGWVVGARRDVT
                     "
     gene            7859..>9397
                     /locus_tag="VFE39_02010"
     CDS             7859..>9397
                     /locus_tag="VFE39_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6257402.1"
                     /translation="MPDVVAPDVAPPGWIRRLVRACFRHRRTAVGALVVSVLGVSLDA
                     VGPLLTRVAVDDAVAGSTAVLGTVVAAFLVLALIRFVAAFLRRFLAGRLALDVQHDLR
                     RQVFAAVQRLDGQRQDSMRTGQVVSRAITDLQLVQSLLQLVPVALGGVVLVVVSIGAM
                     LWLSPMLTLVALVLLPGATWITLRTRTTLFPATWSAQQRAADIAQQVEETVTGVRVVK
                     GFGQESREVGALERGALRLFAERMRAARLTARLNPTLLALPTLGQVGVIGFGGWLALG
                     GSISLGTFLAFTTYVAQLVGPARLLASLVVSAQLARAGVQRVHDLVDSRPDVVDPAEP
                     VALPRGPLSVELHHVSFGYGSREPVLDDVSLRVEPGETLALVGTAGSGKSTVALLLPR
                     FYDPRAGELRLGGVALPRLRLADLRHELGVVFEEAFLFSDTIRANIAYGRPDASDEEV
                     RAAARAAQVEPFVEPLPDGYDTMVGERGLTLSGGQRQRIALARAILTDPRVLVLDDAT
                     SAVDT"
BASE COUNT         1259 a         3319 c         3378 g         1441 t
ORIGIN      
        1 cggatgcacc cgctgaagtg cggatgatgc gggcgcctga aagaccaagc gccctctgag
       61 cagggacaat gcggttgccg acgccacacg ttccccggcc cggaaggcac ctggtagatg
      121 cgagcttccc acaacctcag cagtgtgtgc ctcagcagcg tgtcggtgag ctgcgacgag
      181 ccgagtctcg tgtcgggccc ggggttgttg cccgcggtgg tgctggcggg cggcatagcg
      241 cttcttccag ccgatcaccg tgggctggga aaccccgacc cgctcaacga tctccttcac
      301 cggcaccgca tccgcggcca ggagcacgaa tccacgcccg gcgcgccagc cccgctggca
      361 acgtcggcgc acgcagccac gactctagaa cctcgcggtc acgcgccgac acttccacgg
      421 tcacagtcaa caccacgaac catgaccgag aaccgatcgc caggtaaagc tgcctaatca
      481 ccgcgacact agctacttcg ccctgaagaa gtagcgaccg ttgagcgtct gacctgcgtg
      541 ttctgtcggc gcgcctcctc tgtacacgtt cgaatgaacg ggagaatgac gcgtagggcg
      601 gtgagctggg gaaacggggt ctgatgctgc ggacacgagc acccgaggtg agtctgtggg
      661 aggcggtgct cccggacgag gtgctgcgcc tgccggagga gctggcccgg gtcgacgcgt
      721 tgttggacga tccggtgttc ttcgccccgt tcgcaccgtt cttcgacccg cggttgggcc
      781 gtccctccac gccgatggag gtctacctgc ggttgatgtt cctcaagttc cgctaccggc
      841 tcggctacga aaccctgtgc cgcgaggtcg ccgattcgat cacgtggcgg cggttctgtc
      901 ggatcccgat cgatggtcgg gtgccgcacc cgaccacgct gatgaagctc accacccgct
      961 gcggcactac ggcggtcgac gggtgcaacg aggcgttgct ggccaaggcg gcgcaggcga
     1021 ggctgctgcg cactacccgg ctgcgcgccg acaccacggt cgtcccggct gatgtcgcct
     1081 acccgaccga ttcgggattg ttggccaagg cggtgcggcg tatcgccgcg gccgggcgtc
     1141 gggtgcaggc tgcgggcgga gcgacccgca cccgggttcg ggaccgtagc cgctcggcgg
     1201 gggctcgggc ccgggagatc ggggcgaagc tgcggctgcg cgccgcgcag acccgcgacg
     1261 aggcccaggc cgtggtcaag cggatcaccg gcgagctggc tgggctggcc gaacgggctg
     1321 cggcggaggc cgaggcgctg ctgggcaacg cccgccgggc attgcgccgc gcgcagacca
     1381 aggccgcaca gctcgccgcg gccggtgaac aggatccgga cgctggacgg cggcgagggc
     1441 gactgcaccg ggccgtgaac gacctgaccg acctgctggc cgcgacccga cggatcgccg
     1501 cccagacccg tcaacggctc tcaggaacca cgcccgaggg cgcgacccgg cgggtcagcc
     1561 tgcatgacgg ggacgcccgc ccgatcgcga agggccgatt ggggcggccg gtcgagttcg
     1621 gctacaaggg ccaggtcgtc gacaacgatg acggtgtggt gctcgaccac accctcgaac
     1681 gcggcaaccc cgccgacgca ccgcagctcg cccccgcggt cgggcgggtc atcggccgga
     1741 ctgggcggcg cccgcggacg gtcaccgctg atcgtggcta cggcgaggcc ggcgtcgaga
     1801 acgatctgca tgatctcggg gtccggaccg tggtgatccc ccgcaagggc agacccggca
     1861 acgcccgcca agcccacgag caccgtcgag cgttccgcag aaccgtcaaa tggcgcaccg
     1921 gcagcgaggc ccggatcagc accctcaagc ggcagcacgg gtgggaccgc acccgcctcg
     1981 atcacctcga cggagcccgg atctggaccg gacacggggt cctggcccac aacctggtca
     2041 agatcgcggc cctggccggt tgatcggccg cccaacagag atcaacacaa ccagaccgcg
     2101 gccaggatcg accccacatc cgcgatctcg gcctatttca gggcgaagta gctaggctgc
     2161 cgccgtgagg ttccgcgtcg tcccggccgc gtacgtcctc cccctccggc gcgtcggacg
     2221 gaccgacgag gtgctcctgc agctgcgacg cggcacgggc tacatggacg ggcattgggc
     2281 ggcggccgcc gcgggtcacg tcgaggccga cgagtcggtg cacgcagccg catgccgcga
     2341 agccgaggag gagctgggca tcggcatcgt caccgccgac ctcgtgccgc tcaccgtgat
     2401 gcaccgcacg cacggcaacc accgcgcgat cgacgaacgt gtcgacttct tcttctcgtg
     2461 ccgcacctgg acgggggacc ctcgccgggt ggagcaggcg aagtcggcgg atctgcgctg
     2521 gtgcagcctc gacgacctgc cggagccggt ggtgccgcac gagcttttcg tgctggcggg
     2581 gctgcggaga ggggacctgc cggcgatcgt cagcttcggc ttcgacgcgg ccacctcccc
     2641 cgacgcccgt gcgccccgcc ggtaggacgg cgcgctacac cacccgacgc tccttgccct
     2701 cccagtacgg cgcccggagg tccttcttga ggacctttcc ggtggggttg cgcggcagct
     2761 cgtccagcac gtccaccgac ttcgggcact tgaacgcggc cagctgctcg cggcagtagg
     2821 ccagcagcgc gtcgccgtcg aactccacgc cgggcgcagc cacgacgatc gccttggcca
     2881 cctcgcccca ccgctcgtcc ggcaccccga tcacggcgac gtcggcgacg gtcgggtgct
     2941 cagccagcac ccgctcgatc tcggcagggt agatgttctc cccgccgctg atgatcatgt
     3001 ccttgatccg gtcggtgacg tagacgtagc cgtcggcgtc gatgtgcccg ccgtcgccgg
     3061 accgcagcca gccgtcgggg gtgacggcgg cagccgtggc gtccggcttg ccccagtaac
     3121 cgcccatcag ctgctcggtg cgcacccaga tctcgcccgc ctcacccgtg ggcaccgggt
     3181 cgcccgtggc cgggtcgcgg atctcgatct cgactccgtg gatcgacgtt ccggcggaga
     3241 tcaggcggtg ggcgacggcc gggtcgtcgt ggtcctcggg ccccagcgag ctgacgacgc
     3301 cgctggcctc ggtcatgccg tagacctggt gcatcacgcc ggggaagagc ttcaggcagg
     3361 cccgcatcac cggcaggggc atcggggagg ccccgtagga cagcgccagc agtgacgaga
     3421 agtcgcgatc ggcggcccca ggcacctggg ccatcgctgc catcagtgcc ggcacgagga
     3481 acgtgtgggt gatcctctcg gactcgagaa ccttcaggat tgcgaccggg tcaggcagcc
     3541 gcagcatcat gatctgcgcg ccggcagaga tcgcgagcag cgcgtagctg gtgcccccga
     3601 cgtggtacat cggcattgcc acctgcacgc gcgcgtccgg acccagctcg aagtcggtgg
     3661 cgacgttctc ggcatgggcg agcatgctgc ggtgggtgag catggcgccc ttcgggaagc
     3721 cggtggtccc cgacgtgtag agctggacga agcagtcctc ccggtcggcg ggatacacct
     3781 cggggtcggg ttgccgcgcg gccagccagg cctcgtactc gtcgtccgcg cccccgaccc
     3841 ggatcacccg ctcgacggtc ggcagcttgt cgcgcacctg ctccaccgcg gccgcgaact
     3901 ccgggccgac gaacaggatc cgggccgcgg cgtcgttgat gacgtagacg atctccggag
     3961 gcgccagccg gaagttgacc acggcgttgg ccgtgccgac ctgggcgcag gccagcgtca
     4021 gctcgagaca ggacgggtgg ttgaggtcga ggaccgccac ccggtcaccc ggcaggagcc
     4081 cttccgcgcg ctgagcggcc gcgttctgcc gcacccggcg cgtgagctcc gaccaggtcc
     4141 gggcagtgtc gccgaagcgc agcgcgaccg cgtccggccg ttcggcctcc cagcgcgcga
     4201 gcgccgacgt gaagtccgtg agctgggtgg tcatgcaggc ctccggcgac gggcgggtgc
     4261 gaggtggtgt ggcgcagcgc acacaaggga acccgaatcg taaccagtag gtggggctcg
     4321 ggcgatcgtc gagttccggt actcgagatc acgccctgcc gcgaccgcga ctaccggtca
     4381 gcgccgagtt cttccgagcc cctctgagga acgactgcag ctcggtccag gtgcccgcct
     4441 gcctgtcgcg ctcgctgatc agcgtgggtg cgagcggcca gtcgatggtg aggtgggggt
     4501 cgtcccagcg gacggcgatg tcctccgtcg ggtcgtgttc ggcgtcgatg cggtagcaga
     4561 tgtcggcggt ctcggtgagg gcctggaatc cgtggaggaa cccccggggg atgtagagcc
     4621 ccagcatccg ctcgtcgtcg agccggaacg cggcccagcg gccgaaggtc ggcgagtcgg
     4681 tccgcgcgtc gatgactacg tcgaacaggg cgccggagga gcacctcaca aatttggcct
     4741 cgcccttgcc gaaccggccg tggagtccgc ggatcactcc cttccggcta cgggactggg
     4801 agtcctggac gaaagtgccc ggatcgatgc ccgcggagcg ggcggcggat gattcgaagg
     4861 tcctgctgaa ccagcctcgg tcgtcgtggt gtggcttcgg ctcgaagagc agaacgccgg
     4921 ctagctcggt tcggtggacc tgcacgatcg cgactcccct atcgatctga tcgctaaact
     4981 taccttaacg aaaaggtaga gttcggggac agatcggcct gagtggccgc cttgtcggtg
     5041 atttcggagc tggttgtacc tgcacgcctt cgaccctact gtcctgtgat ggaagctttg
     5101 tccgcttctc agcggggctt caggaagcct ggtgaggctt cgacggaaag ggtggatttc
     5161 cgtgaggttg ccgcccgggc tcgttcgtgc cggaggtgcc ggacgtgggg tacccccgca
     5221 ccgggcgacc cgtgcaactg gctacggtga ctgcgctcgc cccatgccgc catcagcagg
     5281 cgccgaccgc tcggagtccc cacatgagtt ctcccctgca ggtcccccgg atgcgtgcga
     5341 ctgcgcggcg ggtcaaggcg gggggtgagc ggttcctcgg tggtgtgctg ccgggagcgt
     5401 cagcgcggag gctgcgggag cgcgccttcg ccgacgactg ggcggccgcc aacctcgacg
     5461 cccggcaggc caccggtccg ctgtgggtcg tgctcggtga ctcggtcagc caggggatcg
     5521 gcgccaccac ccgcgaggtg ggctacgtcg gcgtcgttca cgagatgctg cgccgtcggg
     5581 acgcgtggcg cgtggtcaac ctgtcgcgag cgggcgcagg cgtggccgac gtcctcgcgc
     5641 gccagctccc tgagctggct gcgctcaccg ccgcggaacc tgccgcactc gtcagctgca
     5701 tcatcggcgc cgaggacctg gcccgtcgca ctcccggcct ggacacttcg ctgcgtcagg
     5761 tgatcgccgc cctgcccacc ggctccgtca tcggcaccct ctcccggggc ggtcgtggtg
     5821 ggccggccgc ggcactcgac gccgtgatcc gcgaggaggc cgcccgccac cagctgcgca
     5881 tcgcccagct gccgtcccgc gccggcgatc ccgggcgcgt ccgcgaccac acgctcctca
     5941 gcgatgtcgg gcatgccgcg tgggccaccg cggtgctgaa ggcgatcgac gggccgcccg
     6001 ccgagatcgc ctccaccacc gatcccgctc ttcccgtcgt accggcagtg cccgacaccg
     6061 attagtccgt cggaccgcat ggacgatcgg agcggacgtg tcatggtccg cgagctgagc
     6121 aagcggttcg ggtcgatcac cgcggtggac ggggtgacgt tctccgtgga gcccggcgcc
     6181 gtcaccgggt tcctcgggcc caacggctcg ggcaagacaa cgaccctgcg gatgatcctc
     6241 gggctggtga cgccgacgtc gggcggcgcg catgtcaacg gggtgccgtt cgccgagctg
     6301 gccaatcccg cgagggtggt tggcgcggtg ctcgaggcgc aggggttcca cccgggccgc
     6361 acggcacgca agcacctgct ggtctgcgcg gccgcgatcg gtgtgcccga cgccgccgtc
     6421 gaccacgtgc tcgggtccgt cgggctggct cacgccgccg accgcggcgt cggtggctac
     6481 tcactgggaa tgcggcagcg gctggccctc gccgtggctc tgctgggcga cccgcaggtg
     6541 ctgatcctcg acgagccggc caacgggctc gaccccgagg gcatcgcctg gctacgcgct
     6601 ttcctgcgcg gcttcgccgg acagggccgc agtgtgctcg tgtccagcca cctgctcgcc
     6661 gaggtggagc agaccgccga ccacctggtc gtgatcagcc ggggccggtg tgtcttccag
     6721 gggtcgctcg cgcagctgcg cggttcgcgg cagacgcggg tgctggtgca gtgccccgcg
     6781 cccgcgcgcc tggccgacgc gttggcccgc accggcctcc tggagatcga cacgctcccc
     6841 gacgggcggc tcggcgtggc cggcaccgat cccgtccacg tcggggacgt ggctctggcc
     6901 gcaggcgtgg cgatccacgg gttggtggag gagaagatcg atctggagca gctgttcttc
     6961 cagctcacca gcggaccatg gcgggccggc tgatgggccg gctgctgcgc gccgaggtgc
     7021 gcaagacgtt ctccacgaag ctctggtggg cgctgctcgt gcccgtggcc ctgctgtcga
     7081 tcgtcatcaa cctgttcggc gggctgttcg ctgcggcgat ccccaacaag ggcgcgggcc
     7141 tgccgctgct gctgggttcg ctggcctacg cgctgggcct gaccggggtg ttcgcggcct
     7201 gctacggcgt cgtcgccatg gctgccgagt tccgccaccg caccatcacc acgacctacc
     7261 tgacggagcc tcggcgcgga cgggtgctgc tcgccaagat ggcgaccagt gccgccgtcg
     7321 gggccctcta cgccgcggtg gtcgcgctcg tgggcgtact ggccggcctc gcgggtcagg
     7381 gcggcgccgc ccccgccgtt gaccagctgc tcgcggtcag cgcgatcggg atggcggtgg
     7441 cggcgctgtg gtccgcgctg ggatccgcgc tgggcaccgt gatcagcaac caggtgagcg
     7501 ggctcgtcgg tatcctggtg tacctgctgc tcggggagct gctgatctcc gcgctgctca
     7561 acagcgccaa ctcggaggct gtcccgcggc tcaccgccta cctcccgggc aacgcgggtg
     7621 acgtggcgat ctacgacatt cccgcgcacg tgctcgcggg ctccgacctc gggccgacgg
     7681 tggtcgagac gctcgccggt gtcaccgcgc cgccgccgtg gtggggcgcg ctgctcgtgc
     7741 tcgcggcctg ggtcgccgcg gtggccgcgg ccgggtgggt ggtgggcgcg cgccgcgacg
     7801 tgacctgaca tgccggagcg gccggacgaa tgtctgagcc gcaacatacg gtgtcgttgt
     7861 gcccgacgta gttgcgcccg acgttgctcc acccggttgg atccgccggc tcgtccgcgc
     7921 ctgcttccgg caccgccgca ccgccgtcgg cgcgctcgtg gtgtcggtgc tcggcgtcag
     7981 cctcgacgcc gtcgggccgc tgctcacccg agtggcggtc gacgacgccg tggcgggttc
     8041 cactgcggtg ctcggcacgg tggtggccgc cttcctcgtg ctggcgctga tccgcttcgt
     8101 cgcggccttc ctgcgccgct tcctggccgg caggctcgcc ctggatgtgc agcacgacct
     8161 gcgccggcag gtgttcgccg ctgtgcagcg cctcgacggg cagcgtcagg actcgatgcg
     8221 caccggccag gtcgtctccc gggcgatcac cgacctgcag ctcgtgcagt cactgctgca
     8281 gttggtgccg gtggcgctcg gcggcgtggt gctcgtggtg gtgtcgatcg gcgcgatgct
     8341 gtggctctcc cccatgctca cgctggtggc gctggtgctg ctgcccggcg ccacctggat
     8401 caccctgcgc accaggacca cgctgttccc cgccacctgg tccgcgcagc agcgggccgc
     8461 cgacatcgcg cagcaggtcg aggagacggt caccggcgtc cgggtcgtca agggtttcgg
     8521 acaggagagc cgcgaggtcg gcgcgctgga gcggggcgcg ctccggctct tcgccgagcg
     8581 catgcgggcc gcccggctca ccgcccggct caaccccacg ctgctcgcgc tgcccacgct
     8641 cgggcaggtc ggggtgatcg ggttcggcgg ctggctcgca ctgggcggtt cgatcagtct
     8701 gggcacgttc ctggccttca cgacctacgt cgcgcagctc gtgggccctg cgcggctgct
     8761 cgcctcgctc gtggtgagcg cgcagctcgc cagggccggg gtccagcgcg tgcacgacct
     8821 ggtcgactcc cgacccgatg tcgtcgaccc ggcggagccc gtcgcgctgc cgcgcggccc
     8881 cctgtcggtg gagctccacc acgtcagctt cggctacggc agccgggagc cggtgctgga
     8941 cgacgtctcg ctgcgcgtgg agcccggtga gacgctggcg ctggtgggca cggccgggtc
     9001 cgggaagtcc acggtcgcgc tgctgctgcc gcgcttctac gacccacggg cgggcgagct
     9061 ccggctgggc ggtgtggcgc tgcctcggct gcggctggcc gacctgcgcc acgagctcgg
     9121 cgtcgtgttc gaggaggcgt tcctgttctc ggacaccatc cgcgccaaca tcgcctacgg
     9181 ccgccctgac gcgagcgacg aggaggtgcg ggcggcggcg cgagcggcac aggtggagcc
     9241 cttcgtcgag ccactgcccg acggctacga cacgatggtc ggggagcgcg gcctcacgct
     9301 gtcgggtggg cagcgccagc gcatcgccct cgccagggcg atcctcaccg acccgcgggt
     9361 cctcgttctc gacgacgcca cctccgcggt cgacacc
//
LOCUS       DASRSC010000042         6161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_832122,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000042 DASRSC010000000
VERSION     DASRSC010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832122"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2399
                     /locus_tag="VFE39_02015"
     CDS             <1..2399
                     /locus_tag="VFE39_02015"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6257403.1"
                     /translation="LSQKITVDARGEILELKSTVNTMVDQLSSFADEVTRVAREVGTE
                     GMLGGQAQVRGVSGTWRDLTENVNQLASTLTTQLRAISAVSTSVASGDLTQQIKVAAF
                     GEVAELKDNINQMIVALRETTEENAAQGWLDSNLVRIGGLLQGQRDLGEVCQMIMNEV
                     APLVKAQVGAFFLAAEPASASGAARWVMSGGYAMTPGDPPLSFGSGDGLVGQAAATRQ
                     ILRVDEVPADYLMIRSGVGTTAPRAVVVLPVQFEGESLGVIELGSVSPFSALHLTFLE
                     RLVATIGVAITTIRANRRTEELLAQSQGLAMELQDQSAELQRTNAELEEKAEQLSEQN
                     RNVEIKNMEIDAARRGVEEKAQQLALASQYKSEFLANMSHELRTPLNSLLLLSRLLAD
                     NPNNNLTDKQTEFASTIYSAGSDLLRLIDDILDLSKIEAGRVDVDPAPVDLTQVRSYV
                     EQAFRPQAEDKGLELRVRTDPELPETIISDAQRLQQILRNLLANAVKFTDAGHVELSI
                     GIVPPGMLHGVPTLDAARTVIAFAVRDTGIGIPREKLDMIFEAFQQADGTTSRKYGGT
                     GLGLSISKELARMLGGKIEVTSGPDEGSVFTLLLPDELPPVTAAAARMTPRRGEELSI
                     PERPAPTGPPILRSTPGGPAGGRSTPELSGVTVLIVDDDVRNVFALTSALELHGLAVL
                     YADNGTDGIRLLTQHPEVDVVLMDSMMPDIDGNETTRRIRALPQGRELPVVFLTAKAM
                     PGDRESSLAAGATEYVTKPVDLDELLSLMAAWVHPRPGSAVATARANGAQAGAGGSGE
                     "
     gene            2396..2935
                     /locus_tag="VFE39_02020"
     CDS             2396..2935
                     /locus_tag="VFE39_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6257404.1"
                     /translation="MTTRQPARVLAVDDRRDNLLALQAILEGLPIEIVSVTSGEDALK
                     RLLVEDYAVILLDAHMPGMDGFETAGHVKQRERTRHIPILFLTAVDYDPHLAFRGYQA
                     GAVDYITKPFDPWVLRSKVAVFVDLWTMHTQLAERAAECGSLRAAVDDALELLDSSDP
                     TATERARERLAAVRSARVR"
     gene            complement(2952..4055)
                     /locus_tag="VFE39_02025"
     CDS             complement(2952..4055)
                     /locus_tag="VFE39_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6257405.1"
                     /translation="MTVPGGCDRALVVESPGVAAVREVPTTTGPVALATICTGVSAGT
                     ELSFLTGMHPALHAGLDTELGLFRDDLPAIPYPVTRLGYMEVARVVADDVGAGPAPGS
                     IVAATYGHRTGYRIDPLAERVVILPADLDPLLGVYVAHMGPICANGLLHAAADLFGAD
                     VRALGDGVRGRRVAVVGGGVVGLLTGLFARRHGAAEVVVLDPTQARRAAATGLGLEAL
                     DPGERDPAVLLKTRWRHGPGDRGADVVFQCRGRTAALALALRLLRPQGTVVDLAFYTD
                     DGTALRLGAEFHHNGLTVRCAQIGRVPRGTDHVWDRERLSAETIDLLLADGAAVREHM
                     ITDVLPLEAGPALLAELAARRRHVIQAVLTVDA"
     gene            complement(4052..5035)
                     /locus_tag="VFE39_02030"
     CDS             complement(4052..5035)
                     /locus_tag="VFE39_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6257406.1"
                     /translation="MAVRAHGHSAVCRIGFVGTGAVARRHARVLTGFPDVELVAAMDV
                     DPDRAAAFTEAHGGRPVPDLGALLTAGLDAVYVCVPPYAHGPAEVQAAAAGMALFVEK
                     PLAADEATAEWVGRRIATAGVVTRVGHHWRCAEPVRRARQLLSGRRIRLVSGVWLDRV
                     PPVPWWSDRARSGGPLVEQAVHVLDLARFLVGEVTEVHAATAGSLPGGADLATGALLC
                     FRNGAVGTLATTCVLDAKHRTGLEIVADGLFVGVGEDWLDIRDGDSGHRADYDVMAPR
                     IAVDRAFIDAVLGRPVDHDRDAPDHADALRSHRLACALARSSESGRAETVR"
     gene            5088..>6161
                     /locus_tag="VFE39_02035"
     CDS             5088..>6161
                     /locus_tag="VFE39_02035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6257407.1"
                     /translation="MTMTVLVNAGPWLAVPPAGYGGIEKVVAALVPELRRRGVRVVLA
                     TVGSSTLQADDLVSVFDDGQFAQLQRPYNRVMGVAAAHLHRVVSELRRRDDVVLVHDH
                     QEAFGLTVLSALGSGAPPVLHTLHWDLAKHPELYGRFDGGGNVWVNGVSATQLATAPP
                     TLRAVSVGHVHLATPLAVGADRRPYPRKAGHLVQVGRITPSKGQHVAARVAHRIRRDL
                     VLAGPVGPYHHAAALLHADEGNPDVRYWREQVEPLVDGDRVRWAGTLQGTELDRLVAT
                     ASAAAFPIDWEEPGGTAVVEALALGTPVVGFRRGCLPELVEHGRTGLLVDPGDEYEFA
                     GALEVADALDPRESRRVAARRFTP"
BASE COUNT          918 a         2233 c         2204 g          806 t
ORIGIN      
        1 acctgtcgca gaagatcacg gtggatgccc ggggtgagat cctggagctg aagtcgacgg
       61 tgaacacgat ggtggatcag ctgtcgtcgt tcgccgacga ggtcacgcgc gtggcgcgtg
      121 aggtaggcac agagggcatg ctcggcggcc aggcccaggt ccgcggcgtg tcgggcacgt
      181 ggcgcgacct caccgagaac gtcaaccagc tcgcctccac gctcaccacg cagctgcggg
      241 cgatctcggc cgtgtcgacc tcggtggcca gcggcgacct gacccagcag atcaaggtgg
      301 ccgcgttcgg ggaggtcgcc gagctcaagg acaacatcaa ccagatgatc gtggcgctgc
      361 gcgagaccac cgaggagaac gccgcgcagg gctggctgga ctccaacctg gtccgcatcg
      421 gcggcctgct acagggccag cgcgacctcg gcgaggtctg ccagatgatc atgaatgagg
      481 tcgcgccgct ggtgaaagcg caggtcgggg cgttcttcct ggccgccgag cccgccagcg
      541 cctccggcgc cgcgcgctgg gtgatgagcg gtgggtacgc gatgacgccg ggcgatccgc
      601 cgctgtcctt cgggtcgggg gacggcctcg tcggccaggc tgccgccacg cgccagatcc
      661 tgcgggtcga cgaggtgcct gccgactacc tcatgatccg ctccggcgtg ggaaccactg
      721 cgccccgcgc cgtggtcgtg ctcccggttc agttcgaggg cgagtccctc ggggtgatcg
      781 agctggggtc cgtgtcgccg ttctcggccc tgcacctgac gttcctcgag cgcctcgtcg
      841 ccaccatcgg cgtcgcgatc accaccatcc gggccaaccg gcgcaccgag gagctgctcg
      901 cgcagtccca gggcctggcg atggagctgc aggaccagtc ggcggagctg cagcgaacca
      961 atgccgagct ggaggagaag gccgagcagc tctccgagca gaaccgcaac gtcgagatca
     1021 agaacatgga gatcgacgcc gcccgccgtg gcgtcgagga gaaggcccag cagctcgcgc
     1081 tggccagcca gtacaaatcg gagttcctgg cgaacatgag ccacgagctg cgcacacccc
     1141 tcaactcgct gctgcttctc tcccgcctgc tcgccgacaa cccgaacaac aacctcaccg
     1201 acaagcagac cgagttcgcg agcacgatct acagcgccgg ctccgacctg ttgcggctga
     1261 tcgacgacat cctcgacctc tcgaagatcg aggccggccg ggtggacgtc gacccggccc
     1321 cggtggatct cacgcaggtg cgcagctacg tcgagcaggc gttccgcccc caggccgagg
     1381 acaagggcct ggagctgcgg gtcagaaccg atccggagct gccggagacg atcatcagcg
     1441 atgcccagcg gctgcagcag atcctgcgca acctcctcgc caacgccgtg aagttcaccg
     1501 acgccggcca cgtggagctc tccatcggca tcgtcccgcc cggcatgctg cacggtgtac
     1561 caacgctcga cgcggcgcgc acggtgatcg ccttcgccgt ccgggatacg gggatcggga
     1621 ttccgcgcga gaaactggac atgatcttcg aggcgttcca gcaggccgac ggtacgacga
     1681 gccgcaagta cggcggtacc ggcctcgggc tctcgatctc taaggagctg gcccgcatgc
     1741 tggggggcaa gatcgaggtg acttccgggc ccgacgaagg atccgtgttc accctgctgc
     1801 tgcccgacga gctcccgccc gtcacggcgg cggcagcccg gatgacaccc cggcgcggcg
     1861 aggagctgtc cataccggag cggcccgcgc ccaccggtcc cccgatcctg cgcagcaccc
     1921 ccggcggccc ggcgggcggg cggtccacgc ccgagctctc cggcgtcacc gtcctgatcg
     1981 tggacgacga cgtgcgcaac gtgttcgcgc tgaccagtgc gctggagctg cacgggctgg
     2041 cggtgctcta cgccgacaac ggcaccgacg gcatccggct gctcacccag catcccgagg
     2101 tggacgtggt cctgatggac tcgatgatgc ccgacatcga cggcaacgag acgacgcgcc
     2161 ggatccgggc gctgccccag ggccgcgagc tgcccgtcgt gttcctcacc gcgaaggcca
     2221 tgcccggtga ccgggagtcc agcctcgcgg ccggggccac cgagtacgtc accaagccgg
     2281 tggatctcga cgagctgctc tccctgatgg ccgcctgggt gcacccgagg ccgggttccg
     2341 cggtggcgac cgcacgggcg aatggtgcgc aggccggtgc cggggggtcg ggcgaatgac
     2401 caccaggcag ccggccaggg tgctggccgt ggacgaccgg cgtgacaacc tgctggcgtt
     2461 gcaggcgatc ctggaggggc tgccgatcga gatcgtgtcg gtcaccagcg gcgaggacgc
     2521 actcaagcga ctgctggtgg aggactacgc ggtgatcctg ctcgacgccc acatgcccgg
     2581 catggacggc ttcgagaccg cgggccacgt caagcagcgc gagcggacgc ggcacatccc
     2641 gatcctgttc ctcacggccg tggactacga cccgcacctg gcgttccgcg ggtatcaggc
     2701 cggtgcggtc gactacatca cgaagccgtt cgacccctgg gtgctgcgct cgaaggtcgc
     2761 ggtgttcgtg gacctctgga cgatgcacac ccagctcgcc gagcgggcgg cggagtgcgg
     2821 gtcgctgcgc gccgccgtgg acgacgcgct ggaactgctc gacagcagtg atccgactgc
     2881 gaccgagcgg gcgagggagc gactcgccgc ggtgcgttcg gcgcgggtcc gatagcagcc
     2941 tggcggacca cctacgcgtc gacggtgagc acggcctgga tcacgtgccg gcgccgggcg
     3001 gcgagctcgg cgagcagcgc gggtcccgcc tccagcggca gcacgtcggt gatcatgtgt
     3061 tcgcgcaccg cggcgccgtc cgcgagcagc aggtcgatgg tctcggcgga gaggcgctcg
     3121 cggtcccaca cgtggtcggt gccgcgcggc acccggccga tctgggcgca gcggaccgtc
     3181 aggccgttgt ggtggaactc cgccccgagc cgcagcgccg tgccgtcgtc ggtgtagaag
     3241 gccaggtcca ccacggtgcc ctgcggccgc aacaggcgca gggccagcgc gagcgccgcc
     3301 gtccgccccc ggcactggaa cacgacgtcg gctccacggt caccgggacc gtgccgccag
     3361 cgggtcttga gcagcacggc ggggtcgcgc tcgccgggat cgagtgcctc gagtccgagc
     3421 ccggtggcgg ccgcccgccg ggcctgcgtc ggatcgagca ccaccacctc cgccgcgccg
     3481 tgccggcggg cgaacaggcc cgtgagcagc ccgaccaccc cgccaccgac gaccgccacc
     3541 cgtcggccgc gcacgccgtc gcccagcgcc cggacgtcgg caccgaacag gtcggcggcg
     3601 gcgtgcagca gcccgttggc gcagatcggg cccatgtgcg cgacgtacac ccccagcagc
     3661 gggtcgaggt cggcgggcag aatcaccacc cgctcggcga gtgggtcgat gcggtagccg
     3721 gtgcggtgcc catacgtcgc ggccacgatc gagccgggag cggggccggc gcctacgtcg
     3781 tcggccacca cccgcgccac ctccatgtat cccagccggg tcaccgggta ggggatggcg
     3841 gggaggtcgt cgcggaacag acccagctcg gtgtcgaggc ccgcgtgcag cgcggggtgc
     3901 atgccggtga ggaagctgag ctcggtcccg gccgacacgc ctgtgcagat ggtcgcgagc
     3961 gcgaccggcc cggtcgtcgt cggcacctcg cgcacggcgg ccacgcccgg cgactcgacc
     4021 accagcgcgc gatcgcagcc gccgggaacg gtcatcgcac cgtctcggcc cggcccgact
     4081 ccgacgaccg cgccagagcg caggccagcc gatggctgcg cagcgcgtcg gcgtggtcgg
     4141 gcgcgtcccg gtcgtggtcg accgggcggc ccagcacggc gtcgatgaac gcccggtcta
     4201 cagcgatgcg gggcgccatc acgtcgtagt cggcacggtg cccgctgtca ccgtcccgga
     4261 tatcgagcca gtcctcgccg accccgacga acaggccgtc ggccacgatc tcgagccccg
     4321 tgcggtgctt cgcgtcgagc acgcaggtgg tggccagagt gccgacggcg ccgttcctga
     4381 agcacaacag cgcacctgtc gccaagtcgg caccacccgg gagcgagccc gccgtcgcgg
     4441 catgcacctc cgtcacctcg ccgacgagga agcgggccag gtcgagaacg tgcacagcct
     4501 gttcgacgag cggccccccg gaccgcgccc gatcggacca ccacggcacg ggcggcacgc
     4561 ggtccagcca cacgccgctg accagccgga tccggcgtcc ggatagcagc tgccgggccc
     4621 gccgcaccgg ctcggcgcac cgccagtggt gccccaccct ggtcaccacg cccgcggtgg
     4681 cgatgcgtct gcccacccac tcggcggtgg cctcgtccgc cgcgagcggc ttctcgacga
     4741 acagcgccat cccggctgcg gcggcctgaa cctccgccgg gccgtgtgcg taaggcggca
     4801 cgcagacgta gacggcgtcg agccccgcgg tgagaagcgc gccgaggtcc ggcaccggcc
     4861 gcccgccgtg cgcctcggtg aaggctgccg cccggtcggg gtcgacgtcc atcgcggcca
     4921 ccagctccac gtcgggaaaa ccggtcagca cgcgcgcgtg ccgccgggca acggcgcccg
     4981 ttccgacgaa gccgatgcgg caaactgcgg aatgaccgtg cgcccggacc gccatgcaag
     5041 gcaaagtacc tgggttcggg cccgttgaaa cgggcactcg atcgaccatg acgatgacag
     5101 tgctggtgaa cgccggcccc tggctggccg tgccgcccgc tggctacggc gggatcgaga
     5161 aggtcgtcgc cgcactggtc cccgagctgc gccggcgcgg cgtgcgggtg gtgctggcga
     5221 ccgtcgggtc gtcgacgctc caggccgacg acctggtctc cgtattcgac gacgggcagt
     5281 tcgcccagct acagcgcccc tacaaccggg tgatgggcgt ggccgcagcg cacctgcacc
     5341 gagtcgtctc cgagctccgc cgccgcgacg acgtcgtgct cgtgcacgac caccaggagg
     5401 ccttcgggct caccgtgctg agtgcgctcg ggtccggcgc cccgccggtg ctgcacacgt
     5461 tgcactggga cctcgccaag cacccggagc tgtacggcag gttcgacggc ggcggcaacg
     5521 tgtgggtgaa cggcgtctcg gccacgcagc ttgccacggc gccgcccacc ctgcgggcgg
     5581 tgagcgtcgg gcacgttcac ctggccacgc ccctcgcggt gggcgcggac cgccgcccct
     5641 acccacgcaa agccgggcac ctggtgcagg tcggtcggat cacgccgagc aagggtcagc
     5701 acgtggccgc gcgggtggcg caccgcatcc gccgcgacct cgtgctcgcg ggccctgtcg
     5761 ggccgtacca ccacgctgcg gcgctgctgc acgcggacga gggcaacccc gacgtccggt
     5821 actggcgcga gcaggtggag ccgctggtcg acggtgaccg cgtccggtgg gcgggcacgc
     5881 tgcaggggac ggagctcgac cggctcgtcg cgaccgcctc agccgcggcg ttcccgatcg
     5941 actgggagga gccaggtggg actgccgtcg tcgaggcact cgcgctgggc acgccggtcg
     6001 tcgggttccg ccgcgggtgc cttcccgagc tcgtagagca cggccggacg gggctgctgg
     6061 tggacccggg tgacgagtac gaattcgccg gtgccctcga ggtggccgac gcgctcgatc
     6121 cgcgggagtc ccggcgtgtg gctgcacgtc gcttcacccc c
//
LOCUS       DASRSC010000043         2997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_177856,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000043 DASRSC010000000
VERSION     DASRSC010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177856"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFE39_02040"
     CDS             complement(<1..330)
                     /locus_tag="VFE39_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6257408.1"
                     /translation="MKVRIGIGSLPLASGPGPGPELAELIEELETHGVDSLWLADTAS
                     APTIDPIVGIAFAAGRTSSLKLGTGVLVLPGRNPALVAAQLASLAALAPRRILPAFGV
                     RSVASAER"
     gene            complement(370..1569)
                     /locus_tag="VFE39_02045"
     CDS             complement(370..1569)
                     /locus_tag="VFE39_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005450832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6257409.1"
                     /translation="MPAHPTAHLATLAVHAGNAVDAGSGAIRRPLVMANSYALPDDPS
                     ELSWSGTGTPLYTRNGGANQGWLEEKLAALDGGTAAVALASGMAAQHAVFFAALRSGD
                     HVVSSDVTYVGTYRLLSELLPQKYGITATLVDSADLDAVRAAIRPKTRLVHVETPANP
                     TTRITDVEGVAEIAHAAGALLSVDATFATPVLQRPLALGADVVVHSLTKYINGHGDAM
                     GGAVIGGGPAGTALVEDVRREAMVNVGGVISPFNAWLITRGSVTLPMRMRAHCAGAMA
                     VAEFLEQHPRVAYVAYPGLPSHPQHALAARTLHGGFGGMLAFAVRGDAAAQNRFVAAL
                     RLITSAVSLGHDESLIVHVAGDDERARFYPEEFRRYGHLRFSVGLEDPRDLIADLDRA
                     LTETLEP"
     gene            complement(1598..2422)
                     /locus_tag="VFE39_02050"
     CDS             complement(1598..2422)
                     /locus_tag="VFE39_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HET6257410.1"
                     /translation="MPEPDPFWWRDTEPARPRRVEGGIQINSTRGQVARSWWSQRFIA
                     VLESIGVGGRLSRGRSYARAGQIVSLDVDVGGAVAQVQGSRPQPYRVRIGIPAFGKTE
                     WAAVAQALTDDASYAAALLNGEMPREIEAVFEAVGLTLFPASTRDLAMDCSCPDHAVP
                     CKHLAAVFYVLAERFDADPFQILALRGRDRETLLEDIRARRAAAHAAGPANGAPALAD
                     VIDTFFVAGPGLADRLAGPRTPSDALLDQVPQFAIEVRGGPLTDLLRPAYRALGDE"
     gene            complement(2415..>2997)
                     /locus_tag="VFE39_02055"
     CDS             complement(2415..>2997)
                     /locus_tag="VFE39_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HET6257411.1"
                     /translation="RSGKVVRLEELLAEILDEGDRVLCFTQFTEFGHLLVPHLSARFD
                     TDVAFLHGGTSKKRRDEMVTRFQAGEDGPPIMLLSLKAGGTGLTLTAANHVIHLDRWW
                     NPAVENQATDRAFRIGQRRNVQVRKFVCPGTVEERIDEMITKKKALSQMVVGDGESWL
                     TELSTDTLRELFSLGTEALDDGALDDGEGAGDA"
BASE COUNT          410 a         1049 c         1121 g          417 t
ORIGIN      
        1 gcgctcggcg gaggcgacgg accgcacccc gaaggcgggc aggatgcggc ggggagcgag
       61 cgcggcgagc gacgccagtt gggcggcgac gagcgcggga ttgcggcccg gaagcaccag
      121 cacgcccgtg ccgagcttca ggctgctggt gcgtcccgcg gcgaacgcga tgcccacgat
      181 ggggtcgatc gtcggggcgg acgccgtgtc ggccagccag agtgagtcga cgccatgggt
      241 ctcgagctcc tcgatcagct ccgcgagctc gggtcccggc ccgggaccgg atgcaagagg
      301 aagcgaaccg atgccgatgc ggaccttcac gtgcccgacg ctacgtgagc ggctggtgcg
      361 gccgccgcat tagggctcga gggtctcggt cagggctctg tccaggtcgg ctatcaggtc
      421 gcgggggtcc tccaggccca ccgagaagcg caggtgcccg tagcgccgga actcctccgg
      481 gtagaagcgg gcgcgttcgt cgtcgcccgc gacgtgcacg atcagcgact cgtcgtggcc
      541 gagcgagacc gccgaggtga tcagccggag cgcggcgacg aaccggttct gggccgccgc
      601 gtccccccgc accgcgaacg cgagcatgcc gccgaacccc ccgtgaaggg tgcgggcagc
      661 cagcgcgtgc tgtgggtggg aaggcaggcc gggataggcg acgtaggcca cccgcgggtg
      721 ctgctccagg aactccgcca ctgccatcgc ccccgcgcag tgcgcccgca tccgcatcgg
      781 aagggtcacc gagccgcgcg tgatcagcca ggcgttgaac gggctgatca caccaccgac
      841 gttgaccatc gcctcccgcc ggacgtcctc gacgagcgcg gtgcctgccg gccccccgcc
      901 gatgacggcg ccccccatcg cgtcgccgtg gccgttgatg tacttcgtga gcgagtgaac
      961 gacgacgtcg gcgccgagtg cgagcggccg ctgcagcacc ggggtggcga aggtggcgtc
     1021 cacgctcagc agggcgcccg cggcatgggc gatctcggcg acaccttcga cgtcggtgat
     1081 gcgggtggtg gggttggctg gggtctcgac gtgcaccagg cgggtcttgg gccggatcgc
     1141 cgcccggacg gcgtcgaggt ctgctgagtc gaccagcgtg gccgtgattc cgtacttctg
     1201 cggcagcagt tcgctgagca gccggtaggt gccgacgtag gtgacgtcgg agctcacgac
     1261 atgatcgccg gaccgcagtg ccgcgaagaa cacagcgtgc tgcgcggcca tcccgctggc
     1321 gagcgccacc gcggcggtgc ccccgtcgag cgccgcgagc ttctcctcga gccagccctg
     1381 gttggccccg ccgttgcgcg tgtagagcgg tgtgcccgtg cccgaccagc tcagctcgga
     1441 cgggtcgtcg ggcagtgcgt aggagttggc catgacgagc ggacggcgga tcgccccgct
     1501 gccggcgtcg acggcgttgc cggcgtggac ggcgagggtg gccaggtggg cggtggggtg
     1561 cgcaggcatg tcgggcactc tgccttcgta cccgtcccta ctcgtcgccg agcgcgcggt
     1621 aggcgggccg cagcaggtcg gtgagcggcc cgccgcgcac ctcgatggcg aactggggaa
     1681 cctggtcgag cagcgcgtcg gacggcgtgc gcggcccggc gagccggtcg gcgaggccgg
     1741 ggcccgccac gaagaacgtg tcgatgacgt cggcaagggc cggcgccccg ttggccgggc
     1801 cggcagcgtg ggcggcggcc cggcgggcac gtatgtcctc cagcagcgtc tcgcggtcgc
     1861 gcccgcgcag agcgaggatc tggaacgggt cggcgtcgaa ccgctccgcg agcacgtaga
     1921 acaccgcggc gaggtgcttg cacggcacgg cgtggtcggg gcagctgcag tccatggcga
     1981 ggtcgcgcgt gctggccggg aacagggtga gaccgacggc ctcgaacacc gcctcgatct
     2041 cccgcggcat ctctccgttg aggagcgccg ccgcgtagga ggcgtcgtcg gtcagcgcct
     2101 gggccaccgc tgcccactcg gtcttgccga acgccgggat gccgatccgc acccggtagg
     2161 gctgcggccg cgagccctgc acctgcgcca ccgctccgcc cacgtcgacg tcgagggaga
     2221 cgatctgccc ggcccgggcg tagctgcggc cgcgggagag cctgccgccg accccgatcg
     2281 actccagcac cgcgatgaac cgctgcgacc accagctgcg cgccacctga ccccgggtgc
     2341 tgttgatctg gatgccgccc tcgacccggc gtgggcgggc gggctcggtg tcgcgccacc
     2401 agaacgggtc gggctcaggc atccccggcc ccctcgccgt cgtccagtgc cccgtcgtcc
     2461 agcgcctcgg tgccgaggct gaacagctcg cgcagcgtgt cggtggacag ctcggtgagc
     2521 cagctctcgc cgtcgccgac caccatctgc gacagcgcct tcttcttcgt gatcatctcg
     2581 tcgatgcgct cctcgaccgt gccggggcag acgaacttgc gcacctgcac gttgcgccgc
     2641 tgcccgatcc ggaaagcgcg gtcggtggcc tggttctcca cggccgggtt ccaccagcgg
     2701 tccaggtgga tcacgtggtt ggctgccgtg agggtgagcc cggtgccgcc ggccttgagc
     2761 gagagcagca tgatcggcgg gccgtcctcc ccggcctgga acctggtgac catctcgtcg
     2821 cggcgcttct tggacgtgcc gccgtgcagg aacgcgacgt cggtgtcgaa ccgggccgac
     2881 aggtgcggta cgagcaggtg cccgaactcg gtgaactgcg tgaagcacag cacccggtcg
     2941 ccctcgtcga ggatctcggc cagcagctcc tccagccgga cgaccttgcc cgagcgc
//
LOCUS       DASRSC010000044        11281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_178023,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000044 DASRSC010000000
VERSION     DASRSC010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11281
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178023"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1518)
                     /gene="guaB"
                     /locus_tag="VFE39_02060"
     CDS             complement(1..1518)
                     /gene="guaB"
                     /locus_tag="VFE39_02060"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010316094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET6257412.1"
                     /translation="MTSEAAGLPPKFAKLGLTFDDVLLLPAESDVVPSAVDTSTQVTR
                     RLRIRVPLVSSPMDTVTESRMAIAMARAGGLGVLHRNLSPEEQAAQVEVVKRSEAGMV
                     TDPVTCAPNATLADVDALCAKFRISGVPVTDAGGHLVGIITNRDMRFEMDHSRPVHQV
                     MTHAPLITARVGVTAEAALGLLRRHKLEKLPIIDGHGILRGLITIKDFNKTEKYPLAT
                     KDPDGRLVVAAAVGVGEDAYARAMHLVDAGVDVLMVDTAHGHSRRVLETVAKLRAEAG
                     HTVDVVGGNVATKAGAEALVEAGADAVKVGVGPGSICTTRVVAGVGAPQITAIYEAAL
                     ACAPAGVPVIGDGGIQYSGDISKAIAAGASTVMLGSLLAGTAESPGDLILVGGNQYKT
                     YRGMGSLGAMRSRGGENGPRKSYSKDRYAQDDVLSEDKLVPEGIEGRIPFRGPLGQVV
                     HQLVGGLRSGMGYAGAQTIEQLQQAQLVRITAAGLKESHPHDITMTAEAPNYVAR"
     gene            1713..2090
                     /locus_tag="VFE39_02065"
     CDS             1713..2090
                     /locus_tag="VFE39_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 domain-containing protein"
                     /protein_id="HET6257413.1"
                     /translation="MSPDALPPDPFAGDPHDPALSLDGPDGGDQDGLSEAERDEVVAD
                     LADLAVYQALLEARGVRGIVVDCGDCGEQHFHEWSLLRASLQQLLDEGQMRPHEPAFD
                     PDPTSYVTWEYCRGYADAVLSDS"
     gene            complement(2154..3359)
                     /locus_tag="VFE39_02070"
     CDS             complement(2154..3359)
                     /locus_tag="VFE39_02070"
                     /inference="COORDINATES: protein motif:HMM:NF028061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-D factor RsdA"
                     /protein_id="HET6257414.1"
                     /translation="MGDDDHRFGGNPFEHRPRRSNGSNGHRPDGMKTRSISLDEVGEP
                     LDLVAVQADDELINALAAGMTVSSPGLTGYGPDDRVAAILAAWKADVDVRPIPELVDV
                     DTAVSTVLAARRSARRPRRLAPVAAAAAVVVLALGGLSLGSYNAQPNDTLWAVSKVLY
                     SERAQSVEAAARVEQRITNAKQALVAGKPVVAAQELAQAQADLAGVRPEEGRTELAQV
                     QEFLVAKAAETPPGTPTDPGTPLSTQPSRPVPPGAAVEAPGAAGTGHPLPWRPSGPEV
                     GPSGTPTPEATPDPSAPWRSQQGRDPRDSTSDPRRQRAPAAPSGVESPGSGTSVPPSA
                     PPTSVGSPSNPEPTTGPTTPPATAERSHDPVTTAPAQSMGGTPSSNQGTEGSTGATAA
                     GSATQQPTS"
     gene            complement(3360..3932)
                     /locus_tag="VFE39_02075"
     CDS             complement(3360..3932)
                     /locus_tag="VFE39_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6257415.1"
                     /translation="MSETTDVPDELVTRAMAGDPQAVGHVIAIIRPMVVRYCRARLGR
                     MDRSSVSADDVAQEVCLAVLTALPGYRVQGRPFLAFVYGIASHKVIDAHRAATRNRSE
                     PVADVPDSVESADGPEQRALRVELSGEMSRLLDTLPEKQREILVLRVVVGLSAEETAE
                     AVGSTPGAVRVAQHRALARLRKSMPEEEVV"
     gene            complement(4279..4875)
                     /locus_tag="VFE39_02080"
     CDS             complement(4279..4875)
                     /locus_tag="VFE39_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6257416.1"
                     /translation="MTTVLICDDRRSVREGLTRVMSAVPGVHRIDCVAHGDELLARFS
                     RQPVDVVLVGTQRAVPTGVEATRRLVAAHPQANVIVFGAPDDAGSIAAAIAGGARGYL
                     RWDASRPELVAALAHTLASTAVPTPRAPTDPGVQLTERELQVLRGMSQGKSNGQIGRE
                     LYLSEDTVKTHARRLFRKLGVRDRAQAVAHGFRRGLVS"
     gene            complement(5042..6040)
                     /locus_tag="VFE39_02085"
     CDS             complement(5042..6040)
                     /locus_tag="VFE39_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257417.1"
                     /translation="MIDLLRADAPSEALAAAFGAEPGAPLRVRPGARAVERWYVAVAL
                     GGQGRYAAAAAVLEPLLVRPGVPVAVAAHAAVTLASHRRQLGGHAAARRYDALGLRLA
                     CSVPGDETFAADPHGSGLLAARVDALVGLAADAVGTSAPDTARRMLDAAQRALVAAAG
                     DADGARPSWRPTVRLEWVRAELALARGDAAAAVGPARRALELACGGGSARHVVKSRIV
                     LAVAAAAAGGDPVAAVTELDAAADEALRQEMLPLVWPARLAAADLLGRIPETARNAND
                     GALAMSQESVSGQVSDAARRRHAALATLSVIERRCHPVGRRLVGVATGVAAQRPVM"
     gene            complement(6037..7095)
                     /locus_tag="VFE39_02090"
     CDS             complement(6037..7095)
                     /locus_tag="VFE39_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6257418.1"
                     /translation="MQPEAPAGTGRSTPVLPPSPATDAAEARLTVAAAARRLGIAPAT
                     LRTWDRRYGIGPSEHTPGRHRRYSPEDLARLELMHRALVRGASPADAAGYALSARVDR
                     SVDGAPFRGPDRGGPGGAGEPDDPLLPEPNDGGGRVRVGGRVLRLPGTGRTARGLGRA
                     ALALDAPAVHRLLEASVAAAGVEAAWEDVVRPVLAAIGERWAHNGAGVEIEHLVSECV
                     HPVFGARAAALRPVADARPVLLAAMPDELHTLPLVVLSAALAERGVACRSLGANVPAD
                     ALGAAIRRTAPVAVVLWAQVPGTADANVLLALPHTRPRFRTYAAGPGWADVALPERIA
                     QLASLPAATATISEAVLV"
     gene            7229..7558
                     /locus_tag="VFE39_02095"
     CDS             7229..7558
                     /locus_tag="VFE39_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET6257419.1"
                     /translation="MADTRRLPVPITEIWDWQMHGACRGMDSAYFFHPEGERGPARAN
                     REARAKEVCRRCPVLEPCRRHALSAQEPYGVWGGLSESERDDIIRGHDRTLRMAGVRP
                     RVRDLST"
     gene            complement(7625..9247)
                     /gene="groL"
                     /locus_tag="VFE39_02100"
     CDS             complement(7625..9247)
                     /gene="groL"
                     /locus_tag="VFE39_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943352.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET6257420.1"
                     /translation="MPKQITFDETARRALERGVNQLADAVKVTLGPRGRHVVLDKKFG
                     GPTVTNDGVTIAREIDLEDPFENLGAQLAKSVATKTNDVAGDGTTTATVLAQAMVAAG
                     LRNVAAGANPTALGVGISKAADAVCELLAKKAIPVDDKEHIAQVGTIASREAEIGNLI
                     SEALKTVGKDGVITIEEGSTLATELEITEGLQFDKGYLSPYFVSDPESMEAVLEDTYV
                     LLHRDKISAIADLLPLLEKVLADGKPLLIVAEDLEGEALSTLVVNSIRKTIKVAAVKS
                     PFFGDRRTAFMDDLAVATGGTVINPEVGLKLSEAGLDLLGTVRRAVITKDTTTLVEGG
                     GSKEAIEARIAQIKAEIEASDSDWDREKLQERLAKLSGGVAVIKVGAATETALKERKH
                     RIEDAVSATRAAVEEGIVPGGGSALVHAAVALDGNLGLTGDEATGVAVVRSALRAPLF
                     WIAANGGLEGAVVVGKVADQEWGHGFNAATLTYGDLMADGVVDPVKVTRSAVVNAASI
                     ARMVLTTESAIVDKPEPPEPAAAGGHGHGHGH"
     gene            complement(9343..9633)
                     /gene="groES"
                     /locus_tag="VFE39_02105"
     CDS             complement(9343..9633)
                     /gene="groES"
                     /locus_tag="VFE39_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET6257421.1"
                     /translation="MANIKPLEDKIVVQASEAETTTASGIVIPDTAKEKPQEGKVLAV
                     GPGRVTDTGVRVPIDVAVGDVVIYSKYGGTEVKYNGEEYLILSARDVLAVVN"
     gene            9977..>11281
                     /locus_tag="VFE39_02110"
     CDS             9977..>11281
                     /locus_tag="VFE39_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HET6257422.1"
                     /translation="MTADDPSATSTLSRRSVLRAGLTASAVLGVPGSVLTGLAGSASA
                     APAASPGNPFTLGVASGEPAPDGMVLWTRLATDPLADDGLGGMGDRTTEVEWELASDE
                     GFNKIVRRGVETTGAELGHSVHVELAGLNAGREYFYRFRAGKDLSPAGRTRTAPPASA
                     MQSLAMCFTSCSQYEHGFFTAYARLAEEEPELILHLGDYQYEYTAGAYVAPGGNVRDH
                     IGPETETLANYRQRYAQYKTDADLQAAHAVAPWLVVLDDHEVDNNWADEVPEKPAVPQ
                     PNFLARREAALRAYWENMPLRQAQRPNGIDMQLYRTVSWGRMANFHMLDTRQYRGDQP
                     CNDTFNSDCTGGTDPARSLPGSEQEKWIAEEFRRSQARWDVLGQQVFFAQVDFTPGAG
                     RGFNPDAWDGYPGSRDRVVDSWTASKARNLLVLTGDVHAHWAA"
BASE COUNT         1578 a         4056 c         4007 g         1640 t
ORIGIN      
        1 ctagcgggcc acgtagttgg gcgcctcggc cgtcatcgtg atgtcgtgcg ggtggctctc
       61 cttgagcccc gcggcggtga tccggaccag ctgcgcctgc tgcagctgct cgatcgtctg
      121 cgctcccgcg tagcccatgc cggaccggag cccgccgacg agctggtgca cgacctggcc
      181 gagcggaccg cggaacggga tgcggccctc gatgccctcg ggaacgagct tgtcctcgct
      241 cagcacgtcg tcctgggcgt agcggtcctt ggagtaggac tttctcggcc cgttctcccc
      301 gccgcgcgac cgcatcgccc cgagcgaccc catcccgcga taggtcttgt actgattgcc
      361 gcccaccagg atcaggtcgc cgggcgactc ggcagtgcct gcgagcaggc tgccgagcat
      421 cacggtggaa gcgcctgcgg cgatcgcctt ggagatgtca ccggaatact ggatgccgcc
      481 gtcgccgatc accggaacgc ctgcgggggc acacgcgagt gcggcctcgt agatcgcggt
      541 gatctgcggc gcgccgacgc cggcgacgac ccgggtggtg cagatcgaac ccggcccgac
      601 gccgaccttc acggcgtccg caccggcctc gacgagtgcc tccgcgcctg ccttcgtggc
      661 cacgttgccg cccaccacgt cgacggtgtg gcctgcctcg gcccgcagct tggcgacggt
      721 ctccagcacg cgccgggagt gcccgtgggc agtgtccacc atcagcacgt ccacccccgc
      781 gtcgacgagg tgcatcgcgc gggcgtaagc gtcctccccg actccgacag ccgcggcgac
      841 gacgagtcgg ccgtccgggt ccttggtcgc gagagggtac ttctcggtct tgttgaagtc
      901 cttgatcgtg atgaggccgc gcaggatgcc gtggccgtcg atgatcggca gcttctccag
      961 cttgtgccgc cgcagcaggc cgagcgccgc ctccgcggtg accccgaccc gcgcagtgat
     1021 cagcggggcg tgcgtcatca cctggtggac gggccggctg tggtccatct cgaaccgcat
     1081 gtcccggttg gtgatgattc ccacgaggtg cccgcccgcg tcggtgacgg gaaccccgga
     1141 gatgcggaac tttgcgcaca gcgcgtcgac gtcggcgagg gtggcgttcg gtgcacaggt
     1201 gaccgggtcg gtgaccatcc cggcctcgga gcgcttgacg acctccacct gggcggcctg
     1261 ctcctcgggg gacaggttgc ggtgcagcac cccgagacca cctgcccggg ccatggcgat
     1321 ggccatccgg gactccgtga cggtgtccat cggcgacgac accagcggca cgcggatccg
     1381 caaccggcgc gtgacctgcg tgctcgtgtc gaccgcgctg ggcaccacgt cggactcggc
     1441 gggcagcagc aacacgtcgt cgaacgtcag gcccagcttc gcgaacttcg ggggcagtcc
     1501 tgcggcctcg ctcgtcatcg ccaccatcct gtcgtcccct acccgtcgtc ctccacagtc
     1561 cgtcgtccga ccgcggggag atttcccgac atggtatctg cgccaccgcg ggggagggca
     1621 tggccgcggc gagtccatca ccacaatgcc gctccgcgct gaacccggtg cgctcggttc
     1681 cccggtcttc cggtgcgggt atcgtcgatg gcgtgtcacc agatgccctt ccgccggacc
     1741 cgttcgccgg tgatccgcat gatcccgcgc tgtccctcga cggcccggac ggcggcgacc
     1801 aggacgggct gagcgaggcc gagcgcgacg aggtggtggc cgatctcgcc gatctggccg
     1861 tctaccaggc tctgctcgag gcccgcggtg tccgcggcat cgtcgtcgac tgtggcgact
     1921 gcggcgagca gcacttccac gagtggagcc tgctgcgggc gagcctccag cagctgctgg
     1981 acgagggcca gatgcggccc cacgagcccg ccttcgaccc cgaccccacg agctacgtca
     2041 cctgggagta ctgccgcggc tacgcagatg cggtgctgtc cgactcctga ccccgttcga
     2101 cgacggcgac ggcccccgat ccgagtggat cgggggccgt cgccgttact gggtcagctg
     2161 gtgggctgct gcgtggccga accggccgcg gtggcgccgg tgctgccctc cgtgccctgg
     2221 ttcgagctgg gcgtgccgcc catgctctgc gccggtgcgg tcgtcactgg gtcatgactg
     2281 cgctccgcgg tggcgggtgg ggtggtcggg ccggtggtcg gttcgggatt actcggtgac
     2341 ccgacgctgg tcggcggcgc agacggcggc accgaggtgc cggagcccgg gctctcgacc
     2401 ccactcggag ccgccggggc ccgctgccga cgcggatcag atgtggagtc cctcgggtcg
     2461 cgcccctgct gactccgcca cggcgctgag gggtcgggag tagcctccgg agtgggcgta
     2521 cccgacggcc cgacctctgg gccggacggg cgccacggga gcgggtgccc agtcccagct
     2581 gctccgggtg cctccaccgc ggcaccagga ggcaccggcc gggagggctg ggtggacaac
     2641 ggggtgccgg gatcggtcgg cgtgcccgga ggggtctctg ccgccttggc gacaaggaac
     2701 tcctgaacct gggcgagctc cgtcctgccc tcctcgggtc ggacgccggc gaggtcggcc
     2761 tgagcctgcg cgagctcctg cgccgcgacc acgggcttcc cggcgacgag ggcctgcttc
     2821 gcgttggtga tgcgctgctc cacgcgggcc gcggcctcga cggactgggc ccgctcgctg
     2881 tagagcacct tggacacagc ccacagcgtg tcgttcggct gcgcgttgta agagccgagg
     2941 gagaggccac ccaaagccag cacgacgacc gcggctgccg cggccacggg ggccagccgt
     3001 cggggacgcc gggccgacct gcgggcggcc aagacggtgg acacggcggt gtcgacatcg
     3061 acgagctcgg ggatcggcct gacgtcgacg tcggccttcc atgcggccag gatcgccgcg
     3121 acgcggtcgt cggggccgta gccggtcagg ccgggcgagg acaccgtcat gccggccgcg
     3181 agggcgttga tcagctcgtc gtcggcctgg accgcgacga ggtcgagcgg ttcgccgacc
     3241 tcgtcgagag agatcgagcg ggtcttcata ccgtcggggc ggtgaccgtt gctgccgttg
     3301 gaccgccggg ggcggtgctc gaacgggtta ccgccgaagc ggtggtcgtc gtcacccact
     3361 cacaccacct cctcctctgg catcgattta cgcaggcggg ccaacgcccg gtgctgggcc
     3421 acccggacgg cgcccggtgt ggacccgacc gcttccgccg tctcctcggc ggacaacccg
     3481 accactacac gcagtacaag tatctctcgt tgtttttcgg ggagcgtgtc gagcagccgg
     3541 ctcatctcgc cggacagctc gacacgaagt gcacgctgct cgggcccgtc ggccgactcc
     3601 accgaatccg gcacgtcagc gaccggttcc gaccggttgc gggtggcggc ccggtgggcg
     3661 tcgatcacct tgtgtgacgc gatgccgtag acgaacgcca ggaacggtcg gccctggaca
     3721 cggtacccgg gcaaggccgt gagcacggcc aaacacacct cctgcgcgac gtcgtcggcc
     3781 gagaccgacg accggtccat ccggccgagt cgcgcccggc agtagcgcac gaccatgggc
     3841 cggatgatgg cgatcacgtg cccgactgct tgcgggtctc ccgccatagc gcgggtgacc
     3901 agctcgtcgg gaacgtctgt cgtctcactc atcgcggact attggccagg cgttactgaa
     3961 catgttcgtc atcatccgtt cgtgaggctg accacggtca gtgctcgacc gtgtcctcag
     4021 tgcggaccac cgtagcgaag gggcaccgcg ctccggggaa ggcatcctcc ccgcgggagg
     4081 agacgcgcca gaacgggcgg acgttgcgaa ctcagagtgg gcaccacccg aatggcggcg
     4141 gccggcgcgc agcgggacgg acaccgcggc gggagggcgc gtggctgcgc tgatggcagc
     4201 cacgtcgcct ggtgtgaccc gggcttacgc ccgcgtggtg ctgtctacct gggagggact
     4261 acagacgcca gatcgggctc aggagacgag accgcgacgg aacccgtggg ccaccgcctg
     4321 ggcgcggtcg cgcactccca acttgcggaa cagccgccgg gcgtgggtct tcaccgtgtc
     4381 ctcggagagg tacagctccc gcccgatctg cccgttgctc ttaccctggc tcatgccgcg
     4441 cagcacctgg agctcacgct cggtgagctg cacaccgggg tccgtcggag cccggggggt
     4501 gggcaccgct gtgctcgcga gggtgtgcgc gagcgcggcg accagttcag gacgggaagc
     4561 atcccaacgg aggtaccccc gagccccacc tgcaatggcg gcggcgatgc tgcccgcgtc
     4621 gtcgggggct ccgaacacga tgacgttcgc ttgcgggtgg gcggcgacca gccgccgcgt
     4681 ggcctcgacc ccggtgggca ccgcccgttg ggttccaacc aggaccacgt ccacgggctg
     4741 ccgggagaac cgggccagca actcgtcccc atgcgcaacg caatcgattc ggtgcacccc
     4801 cggcaccgcg gacatcacgc gggtcaggcc ttcgcggacg ctgcgacgat cgtcgcagat
     4861 gagtaccgtc gtcacgacga ttccttcctg cagcttcctg cagccgggtg atgtgtcacc
     4921 cccctcatcg gcgggtgaca agctcacctt gacacgatca gggccggttt gtgccctcca
     4981 ttcgggatag ttgcgccaag tggcactcgt catgtgggtg acctaggacc gaatggagta
     5041 attacattac cgggcgctga gcagctaccc ccgttgccac ccccaccagg cgcctaccga
     5101 ccgggtggca ccgccgctcg atgacgctca gcgtggcgag cgcggcgtgt cgtcggcgcg
     5161 ccgcgtcgct gacctggcca ctcaccgatt cctgggacat cgcgagtgcg ccgtcgtttg
     5221 cgtttcgcgc cgtttcgggg atgcgtccga gaagatccgc cgcggcgagc cgcgccggcc
     5281 acacgagcgg gagcatttcc tgccgcaacg cctcgtccgc cgccgcgtcg agctcggtga
     5341 cggcggctac cgggtcgccg ccggcggcag ccgcggcgac ggccagcacg atgcgggact
     5401 tgaccacgtg cctcgccgac cccccaccac acgcgagctc cagagcccgt cgcgccggcc
     5461 ccacggccgc cgcggcatcc ccccgcgcca gcgccagctc ggcccgcacc cactccaacc
     5521 gtacggtggg ccgccaggac gggcgagccc cgtccgcgtc ccccgcggca gccacgaggg
     5581 cccgctgggc ggcgtcgagc atccgtcgcg cggtgtccgg cgcggaggtg ccgaccgcgt
     5641 ccgctgccaa ccccaccagg gcgtcgacac gcgcggccag caggccgctt ccgtgcggat
     5701 ccgccgcgaa cgtttcgtca cccgggaccg agcaggcgag gcgcagaccg agcgcgtcgt
     5761 accgccgcgc cgcggcgtgc ccgccgagct gacgacggtg ggaagcgagg gtgacagcgg
     5821 cgtgtgccgc gaccgcgacc gggacgcccg ggcgcacgag cagaggttcc agcacggccg
     5881 ccgccgcggc gtagcgcccc tgcccgccga gcgcgaccgc cacgtaccag cgctccacgg
     5941 cccgcgcacc cggccggacc cgcagcggcg caccgggctc ggcaccgaac gccgccgcga
     6001 gggcttcgga cggtgcgtcc gcgcgaagca gatcgatcac accagaaccg cctcgctgat
     6061 cgtcgccgtc gccgcgggca gtgacgccag ctgggcgatg cgctccggca gcgcgacatc
     6121 ggcccagccc ggcccggcgg cgtaggtgcg aaaccgtggc cgggtgtgcg gcaacgccag
     6181 cagcacgttc gcgtcggccg tcccagggac ctgggcccac agcacgacgg cgacgggtgc
     6241 cgtgcgccgg atcgccgcgc cgagcgcgtc cgcgggcacg ttggcgccca gggaacggca
     6301 cgccacaccg cgctcggcca gcgcggcgga gagcaccacc aggggcaggg tgtgcagctc
     6361 gtcgggcatt gccgccagca gcaccggacg tgcgtccgcg actgggcgca gagccgcggc
     6421 acgcgcgccg aacacgggat gaacgcactc gctgacgagg tgctcgatct cgacgccggc
     6481 gccgttgtgg gcccaccgct cgccgatggc agccagcacg ggccggacga cgtcctccca
     6541 cgcggcctcg acacccgccg cggcaaccga cgcctcgagc agccggtgga ccgcgggtgc
     6601 gtcgagggca agcgccgccc tgccgagccc gcgcgccgtg cgccccgtac cgggcaggcg
     6661 caggacacgg ccgcccacgc ggacgcggcc gccgccgtcg ttcggctcgg ggaggagcgg
     6721 gtcgtccggc tcgccggccc caccgggccc tcctcggtcc ggaccccgaa acggggcgcc
     6781 gtcgacggac cggtcgacgc gcgcggacag cgcgtagccc gccgcgtcgg ccggggacgc
     6841 gccgcgcacg agcgcgcggt gcatcagctc gagcctggcg aggtcctccg gcgagtagcg
     6901 ccggtgccgg ccaggggtgt gctcggaggg accgatgccg tagcggcggt cccaggtccg
     6961 gagcgtggca ggcgcgatgc cgagcctgcg agcggccgct gccacggtca gccgggcctc
     7021 ggcggcgtcg gttgcagggg acgggggcag cacgggcgtg ctccggcccg tgccggccgg
     7081 cgcttcgggc tgcaccggct catcgtgcgg gtccggcgcc gaggaggcaa gccaaagttc
     7141 tcttcatgaa caatgtttgg ccggttatca tgtgggttac ctcggcggtg tcgaggcgag
     7201 caccacagaa cagagcgggg tgagggacgt ggcagacacc cgaaggcttc cggtcccgat
     7261 cacggagatc tgggactggc agatgcacgg ggcctgccgc gggatggaca gcgcgtactt
     7321 cttccatccc gagggcgagc gcggcccggc gcgggccaac cgcgaggcgc gggccaagga
     7381 ggtctgccgg aggtgcccgg tcctggagcc gtgccgccgg cacgcgctgt ccgctcagga
     7441 gccctacggc gtgtggggcg ggctgtcgga gtccgagcgc gacgacatca tccgcggcca
     7501 cgaccggacc ctgcggatgg ccggggtgcg gccccgggta cgcgacctgt cgacctgagc
     7561 cggaacgacg aacggcgggc cgaccccgga aagggtcggc ccgccgtctg gtcggaaggc
     7621 tcgatcagtg cccgtgcccg tgaccgtgtc caccggcagc agccggctcc ggcggctccg
     7681 gcttgtcgac gatcgcgctc tcggtggtga gcaccatccg cgcgatcgac gcggcgttga
     7741 ccaccgcgga ccgggtgacc ttgaccgggt cgacgacccc gtcggccatc aggtcgccgt
     7801 aggtcagggt ggcggcgttg aagccgtggc cccactcctg gtcggcgacc ttgccgacga
     7861 cgaccgctcc ttcgagaccg ccgttggcgg cgatccagaa cagcggagcc cgcagggcgc
     7921 tgcgcacgac cgcgacgccg gttgcctcgt cgccggtcag gccgaggttg ccgtccagcg
     7981 cgacagcggc gtgcaccagt gcggaaccgc cgccgggcac gatgccctcc tcgaccgcgg
     8041 ccctggtggc cgacaccgca tcctcgatgc ggtgcttgcg ctccttgagc gcggtctcgg
     8101 tggcagcacc gaccttgatg acggcgacgc caccggacag cttggccagc cgctcctgca
     8161 gcttctcgcg gtcccagtcg gagtcggagg cctcgatctc ggccttgatc tgcgcgatcc
     8221 gggcctcgat ggcctccttc gaaccgccgc cctcgacgag cgtggtcgtg tccttcgtga
     8281 tgacggcgcg gcgcaccgtg cccagcaggt ccagcccggc ctcggacagc ttgagcccga
     8341 cctccgggtt gatgaccgtg ccgccggtgg cgacggccag gtcgtccatg aatgccgtgc
     8401 ggcggtcgcc gaagaagggc gacttgaccg cagcgacctt gatggtcttg cggatcgagt
     8461 tgaccaccag cgtggacagc gcctcgccct cgaggtcctc ggccacgatc agcagcggct
     8521 tgccgtcggc gagcaccttc tccagcagcg ggaggaggtc ggcgatcgcg ctgatcttgt
     8581 cgcggtgcag caggacgtag gtgtcctcga gcacggcctc catcgactcc gggtcggaga
     8641 cgaagtacgg cgagaggtag cccttgtcga actgcaggcc ctcggtgatc tccagctcgg
     8701 tggcgagcgt ggagccctcc tcgatggtga tcacaccgtc cttgccgacg gtcttgagcg
     8761 cctcgctgat gaggttgccg atctcggcct cacgggaggc gatcgtgccg acctgggcga
     8821 tgtgctcctt gtcgtcgacc gggatggcct tcttcgcgag cagctcgcag actgcgtccg
     8881 ctgccttcga gatgcccacg ccgagcgcgg tggggttggc gccggccgcg acgttgcgca
     8941 ggccggcggc caccatcgcc tgggcgagca ccgtggcggt ggtggtgccg tcaccggcga
     9001 cgtcgttcgt cttggtggcg acggacttgg ccagctgcgc cccgaggttc tcgaacgggt
     9061 cctcgaggtc gatctcgcgc gcgatggtga cgccgtcgtt ggtgacggtc ggcccaccga
     9121 acttcttgtc cagcacaacg tggcggcccc gcgggccgag ggtcaccttg accgcgtcgg
     9181 ccagctggtt caccccgcgc tccagcgcgc ggcgcgccgt ctcgtcgaag gtgatctgct
     9241 tcggcatgtc ctgcctctct tgattctcgg gggaaaacac accgccccgg tggcccgctc
     9301 ggtcgaggga ccaccggggc ggcggttaca gaacagcctg cgtcagttga cgaccgcgag
     9361 gacgtcgcgc gcggagagga tgaggtactc ctcgccgttg tacttgacct cggtgccgcc
     9421 gtacttcgag tagatgacga cgtcgccgac ggccacatcg atcggcacac ggacgccggt
     9481 gtcggtgacc cggcccgggc ctacggccag gaccttgccc tcctggggct tctccttggc
     9541 cgtgtccggg atgacgatgc cggacgcggt cgtggtctcc gcctcgctgg cctggacgac
     9601 gatcttgtcc tcgagcggct tgatgtttgc cacgccgatg acctcccctt cctggggtac
     9661 gtgccttcgc ctacatgatc tgggatgacg gccctggggc gtcccgtcgt cgcgggtgcc
     9721 ggaacgccat ggccgtgccg ttggcactct acccacgaga gtgccaacgc ctcaacgccg
     9781 gtcaccgcta tgacccgaag cggtacgagc aggtgacgac gacgtccgcg gtccaccgtg
     9841 cggccggtac cgtggggtac gacaaggagc cgccaaccgg cgaaacgtcc acatcgggcg
     9901 aaagctcgcc caccgttcat ccggtggagc ggcttgcgcc atccgccgca tgaatgggtg
     9961 acgaacgctg cgctgcgtga ccgcagatga tccctccgcc acctcgaccc tcagccgacg
    10021 cagtgtgctt cgggccggcc tgaccgcctc ggccgtgctc ggcgtccccg gcagcgtgct
    10081 caccggactc gccggctcgg cgtccgccgc tcccgccgca tctcccggca acccgttcac
    10141 cctcggtgtc gcatcggggg agcccgcgcc ggacgggatg gtgctctgga cgcggctcgc
    10201 caccgacccg ctcgccgatg acggcctcgg cgggatgggc gaccggacga cggaggtgga
    10261 gtgggagctg gcctccgacg aggggttcaa caagatcgtc cgcaggggcg tggagaccac
    10321 cggcgccgag ctggggcaca gcgtgcacgt cgagctggcc gggttgaacg cgggacggga
    10381 atacttctac cggttccgcg ccggaaagga tctgtctccc gccggccgta cgcgcaccgc
    10441 gccgcccgca tcggcgatgc agtcgctcgc catgtgcttc acgtcgtgct cccagtacga
    10501 gcacggcttc ttcaccgcct acgcccgcct cgcggaggag gagcccgagc tgatcctgca
    10561 cctcggtgac taccagtacg agtacaccgc aggtgcctac gtggctccgg gtgggaacgt
    10621 ccgcgaccac atcggaccgg agaccgagac cctggcgaac taccggcagc gctacgccca
    10681 gtacaagacc gacgccgacc tgcaggccgc gcacgccgtc gcgccgtggc tcgtcgtcct
    10741 cgacgaccac gaggtcgaca acaactgggc cgacgaggtg cccgagaagc cggcggtgcc
    10801 gcagccgaac ttcctcgccc ggcgggaggc ggcgttgcgc gcctactggg agaacatgcc
    10861 gctgcgacag gcccagcggc ccaacggcat cgacatgcag ctctaccgca cggtctcgtg
    10921 gggcaggatg gcgaacttcc acatgctcga cacccgccag taccgcggcg accagccgtg
    10981 caacgacacg ttcaactccg actgcaccgg agggaccgac cctgctcgct cactgcccgg
    11041 ctcggagcag gagaagtgga tcgccgagga gttccggcgc tcccaggccc ggtgggacgt
    11101 gctcggccag caggtgttct tcgcccaggt cgacttcact cccggcgccg gacgcggctt
    11161 caaccccgac gcatgggacg gctaccccgg ttcgcgggac cgagtggtgg actcctggac
    11221 cgcgtccaag gcacgtaacc tgctggtgct gaccggcgac gtgcacgcgc actgggccgc
    11281 c
//
LOCUS       DASRSC010000045        22250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2798887,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000045 DASRSC010000000
VERSION     DASRSC010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 22250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22250
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2798887"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFE39_02115"
     CDS             <1..519
                     /locus_tag="VFE39_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HET6257423.1"
                     /translation="ARWAVGCRAMAERHLSGETPSVELAAIGRRVCEQEHDLLLALGL
                     PAEPAEYGPPAPGSDLHGRPASAELVGAVVHFLREELMPATEGRLNFLARVAANALDG
                     VGRELRLGPEQERRHAERLARFGVTDQAALAAAIRSREIDADDPALLAAVRAAVTDRL
                     AVANPRYLSHPG"
     gene            861..1205
                     /locus_tag="VFE39_02120"
     CDS             861..1205
                     /locus_tag="VFE39_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6257424.1"
                     /translation="MPFSPSRLRGHREGQPLEFHQLAEISGLTPGRIEEIERGQSTPP
                     TDYETKCLMQALDLAPSELRAQRPTPWGDYCHAVLTYAPRLTPEEIESVASVIRNIRA
                     RRRAEQAAEGAA"
     gene            1202..1474
                     /locus_tag="VFE39_02125"
     CDS             1202..1474
                     /locus_tag="VFE39_02125"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6257425.1"
                     /translation="MTSRIEFDDLVLMDVANAAEVLGSEPARIKELIRVGKVAAVMHG
                     GRWLIAEEEVERVRSLATEGSDLLGPRPNKRRFMPPPRDRDQLPLF"
     gene            1946..2215
                     /locus_tag="VFE39_02130"
     CDS             1946..2215
                     /locus_tag="VFE39_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257426.1"
                     /translation="MNGEPVVRAKKIEGLRFDTMRFKGDYPDVYEAYRKPYAGRERDA
                     QRQLLTELRQPTNRAQVDEAYAEYFAVLDQADRERDPHPLPDIAC"
     gene            complement(2394..2873)
                     /locus_tag="VFE39_02135"
     CDS             complement(2394..2873)
                     /locus_tag="VFE39_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257427.1"
                     /translation="MIAKRFITGLVGAGLVAAATTGVLAGTASAAPAAANVQATSNVK
                     PAEPRNDDRSNANDGRNEGRNDNRSNGPRIDAKAIDSNRNGKINQVTVKNTGRNRISV
                     RVVVGRVSKTQNVDPGRTITVRDPQDRANTATVTASENRGRAITQRLTVPAPRGGRH"
     gene            2976..3797
                     /locus_tag="VFE39_02140"
     CDS             2976..3797
                     /locus_tag="VFE39_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET6257428.1"
                     /translation="MATSQNGYSANDRTMIASYTIPGTTLKIALRKGDVSVVILEYLR
                     RYHVEVESLYNNPQDLWGYAERTIIGSSTTLSNHASGTAADARATRHVLGKRGTFTAR
                     ELQSLNRLLAYFEGVLRHGKDYSGRVDEMHVEINKGPAEVARIANKIRQGAAAPAPTP
                     EDDLTPDQDRLLRVIFDQITGNNFTGWPTWPDGTGESLSLVDYARRDNVEVRQLHVNA
                     NALHQKVDALAERVGQLSSGDGSVAPTTGPASLTDADVNRIATRVIDLLSERTRS"
     gene            3794..4141
                     /locus_tag="VFE39_02145"
     CDS             3794..4141
                     /locus_tag="VFE39_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257429.1"
                     /translation="MTYAKFVAQIVATVASAAIVALTGDGTFSDVELINIANAGVAAF
                     GVLYVGNATTSPIAKTVVAALSAVLTLLVNLVADGVTLSEWLQLVVAALGALGVYAFA
                     NRPATTSPVTPSA"
     gene            4244..4525
                     /locus_tag="VFE39_02150"
     CDS             4244..4525
                     /locus_tag="VFE39_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257430.1"
                     /translation="MVEEVRHHQPGVAAAAVVGAPDDVWGERVVAVVEPVAGQEPTEE
                     EIREFCRQQLAGYKCPRQVVLVPELPRNTVGKVLKRELRELSAVTLTTP"
     gene            4591..5550
                     /locus_tag="VFE39_02155"
     CDS             4591..5550
                     /locus_tag="VFE39_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HET6257431.1"
                     /translation="MVSADPTTAELRLVASGLQFPEGPIVLRDGRVLLVEIQRGTLSR
                     VAPDGAVSVVAELGGGPNGAAIGPDGAAYVCNNGGFEWHDRGGITAPGHQPADYIGGR
                     IQRVVLDGPEAGTVTDLYTECDGRPLRGPNDIVFDAQGGFWFTDLGKSRARDNDTGSL
                     YYALPDGSSITEVVHPLVQPNGVGLSPDGSRVYVAETGPGRVWCWDVAGPGRVRPADG
                     AMGPGGAALLHGFAGYQLLDSLAVDGEGNVCVATLVTGAVSVIAPDGTLLDQIPVPEP
                     DPFVTNICFAGADSRTAYVTSSGRGFLYVTQWHTTGLDLAFSA"
     gene            5547..7295
                     /locus_tag="VFE39_02160"
     CDS             5547..7295
                     /locus_tag="VFE39_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HET6257432.1"
                     /translation="MTTADSAVSAVGRRAALAARFTAPAAEFEVVERLVRGIPTRVYS
                     GGPQTLRELLLAGAAFGERDFLVYGTERWTYAQHLRQVAGLARCLLEEYGLRKGDRVA
                     VTMRNHPEWAPIFYATQVAGLVLVPLNAWWTAPELRYALLDSGARFVVADAERTALIA
                     PHLDELGGIPLVEVRGGDKPAPGARRWADLLASLDPDAAVPDVDVAPDDDATILYTSG
                     TTGRPKGAIGTHRNHCTNIRNTQLGAFIGATIANGGEPPVPDPDAPQPGSLCTFPLFH
                     IAGITSICYSVLVGAKLATQYKWDRVEALELVRRERLTGVSGVPTVMRQLVEDATADP
                     GRVPTLAAISMGGTPIPPDLIGRIDSGFSSLVAPANGYGLTETTSAVVSNSGADYVTH
                     PDSVGRCAPGADVRVVDPVNGADLPDGEVGELWFRGPNIVRGYWNNPEATAAAFTDGW
                     FHTGDLGRVQDGWIYVVDRMKDVVIRGGENIYCAEVEAALFEHPAVGDVAIVGVPHGS
                     LGEEAVAVVHLRPGTQPGPATADDLRRHVGTRLASFKVPAHVVFRDEPLPRTPSGKVL
                     KRDLRGPVAAELGVAR"
     gene            7296..8060
                     /locus_tag="VFE39_02165"
     CDS             7296..8060
                     /locus_tag="VFE39_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6257433.1"
                     /translation="MQPDVRYDVDGHVGVVTLDRPHVHNALRRQTYDELTELVRTTSA
                     RVLVITGAGSSFCSGDDVRELMNGGEGSSPRPAPRLTPAAGALLLSDVPVIAAVNGPA
                     VGWGMEMALMADFRIAGRRARFGELFVKRGLCSDVAGIARLAQLVGRERAAELLFTGE
                     VIDAERAERIGLVGRVVDDEQLLPAALELAGRIAANPPLAVAALKRGLRRALDPDWTD
                     LGMWVSSTLGELFTTEDHREGVRSFLEKREPQYVGR"
     gene            8097..8732
                     /locus_tag="VFE39_02170"
                     /pseudo
     CDS             8097..8732
                     /locus_tag="VFE39_02170"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
     gene            complement(8921..10057)
                     /locus_tag="VFE39_02175"
     CDS             complement(8921..10057)
                     /locus_tag="VFE39_02175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6257434.1"
                     /translation="MVEFVFSEEQQDLRRGVRQFLAERSDEAAVRRLMDTDIGYDPAI
                     WSRLGGELGVLGLAVPEALGGVGGTLVDQAVVAEEFGAALTPGPVFGTVSLAIPALVS
                     LGGADDLLTALVEGTRTAALAVPDRAGRFDAGLVTVTESDGALTGEVRQVVDGSADVL
                     LVAARTGSGVGLFAVEGGERIPLSTLDSTRRQATVRLHAAEGRLLATGAGAERAITHA
                     FRVGAALLATEQVGGAQKLVDLTVAYAGTRLQFGRPIGSFQAVKHRCADMLVLVEHAR
                     STAYHAVWTLQDASDGSAGDDPDLAVSIAQATCSEAYQRVALDTVQLHGGIGFTWEHP
                     THLYVKRSVTDAALLGSAAAHREHIAATVLDTAVSDGALVVAAG"
     gene            complement(10050..11234)
                     /locus_tag="VFE39_02180"
     CDS             complement(10050..11234)
                     /locus_tag="VFE39_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6257435.1"
                     /translation="MDDHSELIEKTAREVVETHDPQSTPPREFLGAVYDAGLAWVHFP
                     VGLGGLGVSRGLQAHADEVLQGAGGPIAFSLNPIGQGMAAPTLLEHAQPGLAAELLRP
                     LATTEHIWCQLFSEPGAGSDLAGLATSAVQDGEEWVVNGQKVWTSLAHLARYALLLAR
                     TDPEQPKHRGLTYFVLDMQAPGVETRPLRQMTGQAEFNEVYLSDARIPDTHRLGAVGN
                     GWRVAMTTLMNERSAIGGGSSVRGAGAIADAVELWARRPELHTAVLRDKLAALWTRAE
                     AQRLTSARAGRAATSGSPGPEGSIAKLVSAELNQHVYEFCMELLGPEAALYGSYELHD
                     VKRGEDYRGPVQQRFLRSRANTIEGGTSEVLRNILGERILGLPGDLRADSGKAWKDIP
                     RG"
     gene            complement(11779..11916)
                     /locus_tag="VFE39_02185"
     CDS             complement(11779..11916)
                     /locus_tag="VFE39_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257436.1"
                     /translation="MDSSPTPSMSRTCPWCASASVIAFSEEGLVRALTRHLEACQGEP
                     E"
     gene            12064..12198
                     /locus_tag="VFE39_02190"
     CDS             12064..12198
                     /locus_tag="VFE39_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257437.1"
                     /translation="MSTVTPDRVAEPGPCLTTAPWGGRLISLLFTATVLLFLLWLFAL
                     "
     gene            12267..13481
                     /locus_tag="VFE39_02195"
     CDS             12267..13481
                     /locus_tag="VFE39_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HET6257438.1"
                     /translation="MNINTQARVPLGRVLALGGLSSFGPLALDLNLPALPQLAEDLRA
                     PVAYAQLSLSVCMIGLALGQLLVGPLADRVGRRRPLLIGVASFAVTAGLCAVAPSIEA
                     LLVLRLLGGLAGGAGLVIARAMVRDLYGGAAAARVFALLMLVSGTAPIVGPVLGGQLL
                     RVTDWRGVFAALALIGAVLFVAALTQRETLPAADRRAGGLRATAAALGDVLVDRTFLM
                     PALVQGLGMCAMFTYIAMGSFVLQDGRALDGALNPQTYAVVFAVNGLGIVLAGRVSAV
                     VVGRVGPRRLLGVGVVLALVAALALLAGALASRSVWALLVPLLLLVSCTGLFMPNATA
                     LALAGQGGRAGTASALLGLLQFTFAAAVPPLASLRGVTATVMAVTILATALAAALVFL
                     AVLRTPVPAPVG"
     gene            complement(13450..14730)
                     /locus_tag="VFE39_02200"
     CDS             complement(13450..14730)
                     /locus_tag="VFE39_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6257439.1"
                     /translation="MAGSTARGDDLARAALVRLVRDEGRRVLATLVRLTGDLQLAEDA
                     VQDATVRALETWSRDGVPEEPRAWLTLTARRRAIDLIRREAARDGKEAGTVVPPEPST
                     PEPPEVLRDDLLRLVFTCCHPSLAVETQVALALRTLCGLSTAEVARALLLPEATMNKR
                     LTRAKQKIARAAIPYRVPEDHELPDRLRGVVATVYLLFNEGYAATAGDDPLRPALVDE
                     AVRLTRLLRELMPGEPSVLGLLALELLQDSRRDARYARDGRPVLLADQDRGLWRHDLV
                     EEGVVLVGEGLRRSPGRPDPYVVQAAIAACHALAPSYPDTDWSAVLSWYDVLLTVQDT
                     AVVRLNRAVAVGEQQGPGAGLAEVDAVTGLEEYALWHAARAELLARLARTDEARAAYA
                     AALALPQNAAQHAHLRQRLRAVDGINPPGPAPGS"
     gene            complement(14731..15084)
                     /locus_tag="VFE39_02205"
     CDS             complement(14731..15084)
                     /locus_tag="VFE39_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6257440.1"
                     /translation="MPSYIALIYNADVDWSAAEHSETMAEYGEFGRAAAAVIRGGAAL
                     YPTSTATTVRVHGGKGGDVVTTDGPYAETKEALTGFYLFECADLDEAVATAARIPGAR
                     DGAIEVRPVIPPMQR"
     gene            15181..16065
                     /locus_tag="VFE39_02210"
     CDS             15181..16065
                     /locus_tag="VFE39_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6257441.1"
                     /translation="MRIASLLPAATEMVAALGRADDLVAVTFECDEPAGVRERAVVVV
                     DTVLPPGLTAAAIDAVVRDRSARGLPLYELDRDALAASSPDLLITQDLCRVCALPAGA
                     VDEALAAIGCDAAVLSIDPHSVDEVFAAITAVGHHIEAPEAAAALVGSLRDRLGAVHD
                     AVAGLPRPRVLVLEWVDPPFLPGHWVPELVRFAGGEPVGGADGGRSVQASWDAIAALP
                     SDVVVVAPCGFGLGAAAEQAATVRDRFPGRPVVAIDSASYVVRAGPRLVDGVEALAWS
                     LHPEAVREPPPARVAVLR"
     gene            16167..16727
                     /locus_tag="VFE39_02215"
     CDS             16167..16727
                     /locus_tag="VFE39_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET6257442.1"
                     /translation="MTGATADLDVIDELMSDHREVTSLLDRIDTTTDPADKRELADIA
                     ITELVRHAVAEEMYVYPAMRENLPDGEEVVKHDTEEHKELERTMKELEGVEATDPRFE
                     QLVGELRRELQHHVEEEESEQFPKFRQRVPHDERVQLREKVEKAKKLAPTRPHPNAPK
                     SPLFHKVVGPGVGLVDRVRDKLTGRA"
     gene            16782..18227
                     /locus_tag="VFE39_02220"
     CDS             16782..18227
                     /locus_tag="VFE39_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HET6257443.1"
                     /translation="MTGPADLGEVRAHTVLADADGTPYSSLRQRPVARAERYALGKAL
                     RKRVPRSSLGEWKPPSDRPDPVQLIRESHRGRVERLVPIRVGRMVASPYGFLRGTAVV
                     MAEDVAQLPSTGITPVVCGDAHLGNFVFYASPERDLVIDLNDFDEAHPGGWEWDLRRL
                     VASIWVAGRHNGASEDGCAAAVSSCVSAYREELRHLADQPLMSRSYERLDVDRLEQET
                     AGPLRAEVERAARRARQRTSDRALPRFTQQVGGRRRIVEEPPLITRVSDSDAELLGVA
                     LDGYLQTLAPHWRRVLGGYTLVDIAHKVVGVGSVGLRAYVALLEGSSPEDVVFLQLKQ
                     ARRSVLARYVHGESAWHRHQGQRVVEYQQALQTVSDPLLGWATVSGRDDDVHQYYVRQ
                     FRNMKGTVALDAIDAAALADYAGVVGHLLAKGHARTSGASMIAGYVGGSAKVDDALCG
                     FARAYADQTEADHTALVAAVARGLLPVERGV"
     gene            complement(18252..18581)
                     /locus_tag="VFE39_02225"
     CDS             complement(18252..18581)
                     /locus_tag="VFE39_02225"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HET6257444.1"
                     /translation="MKRCVLWVLPGMLADQPHGRRLTASERIRLDELERQLGNDDPQL
                     AEALRQGVPPGSPRRAVVAGTAMAAALLVLLAALAGGTTGAASMVLILIVLLGVWALT
                     WHRRARR"
     gene            18624..19454
                     /locus_tag="VFE39_02230"
     CDS             18624..19454
                     /locus_tag="VFE39_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6257445.1"
                     /translation="MAIELTAARPRDRLRRSVRRLTRALGPAMGLSTPALMRHPVWTT
                     PAVEGGGTGVVVVPGFGGVDASMVVLRHWLRRRGYVPVGARLGVNLGCTDALVARLER
                     RVEELVRRTGGPVVLLGHSRGGMLGRLVAVRRPDLVCGLAMLGSPVLDPLDARGMAQH
                     LLRVLVRLSAFGVRGLLEQDCLTGACRDTTVEGLVAPLRVPAVAIYSREDGVVGWRSC
                     RDPEAEWFEVTSSHTGMGTDPVLYDALATRLARWVADRAGGPGDHGPSSCTRPESVVS
                     "
     gene            complement(19409..20326)
                     /locus_tag="VFE39_02235"
     CDS             complement(19409..20326)
                     /locus_tag="VFE39_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6257446.1"
                     /translation="MTRYREICSDLAGRIRSGELTEGSELPGVRELASRFGTTASTVS
                     RAQRALADAGVILTSDRRRSRVAPDGAVAARRFLHSELVFRLAGSDDPALDVVTRALG
                     RAVQAIGAEGSFGGLRAVRQGKADGAAVHLLHHTGVYNAPFARGLLRGWRPHLIHLWR
                     REQGIVVPPGNPRGLTTAADLAGLRVAKRRQGSGTRVLFDRLLLAADLDPDAVAGPET
                     GSHLETGLTVASGVVDAGLGVRSVARDLDLDFVSLVDEDYDVVLGAAALDAAAPLVAT
                     LHDPAVRATITELGGYDTTDSGRVHELGP"
     gene            20365..21192
                     /locus_tag="VFE39_02240"
     CDS             20365..21192
                     /locus_tag="VFE39_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6257447.1"
                     /translation="MRSVVAALSLLLLVACGGPAAAPSAERSLILATTTSTQDSGLLD
                     DLLPAFTADTGWQVKTVAVGSGQAIELGRRGEADVLLVHSPAAEEKFVAEGTAGRRLL
                     VMHNDFVLLGPAADPAGIRGTGVADAMTRIANSGAVFVSRGDDSGTAAREKALWQQAG
                     VIPGGGWYQETGQGMGATLRVAAEKGGYTLSDRATYLSQSDGLTLLNEGDPGLLNVYH
                     VIEMTTKAGDRVQPDAAAAFADWITGPAAQQRIGEFGRAQFAQPLFVPDAGKPEPVP"
     gene            21192..21896
                     /locus_tag="VFE39_02245"
     CDS             21192..21896
                     /locus_tag="VFE39_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6257448.1"
                     /translation="MDVLLDGFVQAVQLLLGGDAATWAITGLTLRISLTATVIAAIVG
                     VPLGAAVALTSFRGRRVVLAAANTGMGLPPVVVGLFVTVLLWRSGPLGAFGLLYTQTA
                     MIIAQAVIATPIVVALVAAAVQQMDPDFLVQMQGLGATRTRALAALLGEARLPLLAAA
                     MAAFGAVVSEVGAAQMVGGNLAGETRVLTTAAVLATSRGQFALAIAFGLILLMIAFAV
                     NLVLTLVGQRQAVPSR"
     gene            21893..>22250
                     /locus_tag="VFE39_02250"
     CDS             21893..>22250
                     /locus_tag="VFE39_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6257449.1"
                     /translation="MTFRIGCRDLRVVVRGRELLHVDRLDVAAGAVLAVLGPNGAGKS
                     TLLRALARLGRHHVSGEVLLDGSPAGPREMRPAVAAVLQRPILRRGTVTANAAAGLRL
                     RGVGRSDAVRRSVPWLD"
BASE COUNT         3028 a         7869 c         8078 g         3275 t
ORIGIN      
        1 gcgcgctggg ctgtgggttg ccgggcgatg gccgagcggc acctgtcggg cgagacgccc
       61 tcggtggagc tggccgccat cggccgccgt gtctgcgagc aggagcacga cctgctgctc
      121 gccctcgggc tgccggccga acccgcggag tacgggccgc ccgcgcccgg atccgacctg
      181 cacggcaggc ccgcctccgc agagctggtc ggcgccgtcg tgcacttcct gcgcgaggag
      241 ctgatgccgg ccaccgaggg ccggctgaac ttcctcgcgc gggtggctgc caacgcgctc
      301 gacggcgtcg gccgtgagct gcggctcggg ccggagcagg agcgcaggca cgccgagcgg
      361 ctcgcccggt tcggcgtcac cgaccaggcc gcgctggccg cggccatccg atcccgcgag
      421 atcgacgcgg atgacccggc gctgctcgcc gccgtccggg ccgcggtgac cgaccggctc
      481 gccgtggcga acccgcgcta cctgtcccat cccggctgat cgggaaggac tgccggaaca
      541 ccgaccgaac aagcggagtg gatcgatgag gatcttttcc ttcgcgtcca tgctggaggg
      601 acggccggtg accgagcccg ctcccccgac caggacgccc cggcttggct gctctacacg
      661 tccggcacga ccggccaagc gaagggcgca gcacgctcgc tgggacgtcg atcaggtcag
      721 ggagcagctg cgggtcatgg cggacaagtt ccgggaagcc aaggactgag gcccccttcc
      781 cgcggccgtc cggctcagcc cgttccaaga tcagataaat ctgagacggc atgcgacggt
      841 agggcgatca ccctggtccc atgcccttca gcccctccag gctgcgcgga caccgtgagg
      901 gccagcctct cgaattccac cagctcgccg agatctcggg cctgaccccg ggccggatcg
      961 aggagatcga gcgcggccag tccacgccgc ccaccgacta cgagaccaag tgcctgatgc
     1021 aggccctgga tctcgcaccc tctgaactac gcgcccagcg acccaccccc tggggcgact
     1081 actgccatgc ggtcctgacc tacgcccctc gcctcactcc cgaggagatc gagagcgtgg
     1141 cgtcggtgat ccgcaacatc cgagcccggc ggcgcgcgga gcaggccgcc gagggagcag
     1201 catgaccagc aggatcgagt tcgacgacct cgtgctcatg gacgtggcca acgcggctga
     1261 ggtcctgggt agcgagccgg cccggatcaa ggagctgatc cgggtcggga aggtggcggc
     1321 ggtgatgcac ggtggccgct ggctcatcgc cgaggaggag gtcgagcggg tccgctccct
     1381 cgccacagag ggtagcgacc ttctcggtcc ccgcccgaac aagcggcggt tcatgccccc
     1441 gccccgggac cgtgaccagc ttcccttgtt ctgacaggta gctgcgctac cccgtaatcg
     1501 ctccgtgatg gggtagtgga gctaccaacc gttcaccgaa ccgttcgtcg cagatttgtc
     1561 tcatgtttct cgtcttcctc gacggaatgg gcccgaacca cattgatgtg acacgctctg
     1621 accagtgccg atggccaggc aaggaccagt cggttcgcac cccacaacgg atgggggtag
     1681 tagtgctacc cgaatcctgc atagtcgccc ttcgttggta ggactgagaa cgatcttcgg
     1741 cccacagccc tagatcaaga tctgactcta gttctactaa ggggtgacct atgcccaaaa
     1801 tcggggccct gcgatccgtc ggcgctgagc acgcactcgt ccaaaacaag gagtccgcca
     1861 cgggtgggat ctctgtggaa gagttcctca gtgcacagga agtggcacgc aagcttcaga
     1921 gttgggctac gcctctatcg ggactgtgaa cggggagcca gttgtccggg ccaagaagat
     1981 cgagggcctg cgcttcgaca cgatgcgctt taagggcgac taccccgatg tgtacgaggc
     2041 ataccgaaag ccctacgccg gccgtgagcg cgacgcccag cgccagctcc tcaccgagct
     2101 gcgccagccc accaatcgcg cccaggtcga cgaggcctac gccgagtact tcgccgtcct
     2161 cgatcaggcc gaccgcgagc gcgaccccca cccgctgccc gacatcgcgt gctgacgacg
     2221 caggccgcga cgaggggctc aaacgcagtt gcccctgctc gcccctcgtc ggggcctggt
     2281 cgctctcgca ccacccgaat ggttccgggc ccgcctggcc ccgccgcgcc cccggcatga
     2341 cgaagggccc gccccggcat tgccaggacg ggcccatctg atggagctac ggctcagtgc
     2401 cgcccgcctc ggggggcggg caccgtgagc cgctgggtga tcgcgcgacc ccggttctca
     2461 gaggccgtca ccgtggcggt gttcgcccgg tcctgcgggt cccgcaccgt gatcgtcctg
     2521 cccgggtcga cgttctgggt cttgctcacg cgacctacga cgacccgaac cgagatccgg
     2581 ttccggccgg tgttcttcac cgtgacctga ttgatcttgc cgttgcggtt ggagtcgatc
     2641 gccttggcgt cgatccgggg gccgttcgac ctgttgtcgt tccgtccctc gttccggccg
     2701 tcgttcgcgt tcgaccggtc gtcgttccgc ggctcggccg gtttgacgtt gctcgtcgcc
     2761 tgcacgttcg cagccgccgg cgccgccgag gccgttcccg cgagcacgcc ggttgtggcc
     2821 gcagccacga gaccagctcc cacgagcccg gtgatgaagc gcttcgcgat cacttatctg
     2881 tctccccttc ggtccccgcg ggtcctgttc gcccgcgcag tgacctatcg ctgccagtaa
     2941 ccgggtcgtt acagcgcgtt taacaaggag gccacgtggc cacatcgcag aacggctact
     3001 cggccaacga ccgaacgatg atcgcgagct acaccatccc cggcaccacg ttgaagatcg
     3061 ccctgcgcaa gggcgacgtc agcgtggtga tcttggagta cttgcggcgc taccacgtcg
     3121 aggtcgagtc gctctacaac aacccgcagg acctctgggg ctacgccgag cgaacgatca
     3181 tcggcagctc cacgaccctc tccaatcacg cctcgggcac ggccgcggac gcgcgggcta
     3241 cccgccatgt tctcggtaag cgcggcacgt tcaccgcccg cgagctgcaa tcgctcaacc
     3301 gcctcctcgc ctacttcgag ggggtgttga ggcacggcaa ggactactcg ggccgtgtcg
     3361 acgagatgca cgtcgagatc aacaagggcc cggccgaggt cgcgcgcatc gccaacaaga
     3421 tccgccaggg cgccgctgcg cccgcgccca caccggagga cgacttgacg cctgaccagg
     3481 accggctgct gcgcgtgatc ttcgatcaga tcaccggcaa caacttcacc ggctggccga
     3541 cctggccgga cggcacgggc gagtcgctgt cccttgtgga ctacgcccgc cgcgacaacg
     3601 tcgaggtccg gcagctgcac gtcaacgcca atgcgctgca ccagaaggtc gacgccctcg
     3661 ccgagcgggt cggccagctc tccagcggcg acggctcggt ggccccgacc accggccctg
     3721 cctccctcac cgacgcagac gtgaaccgca tcgccacgcg cgtcatcgac ctgctgtccg
     3781 agaggactcg atcatgacct acgccaagtt cgtcgcgcag atcgttgcca cagtggcttc
     3841 ggccgcgatc gtcgccctga ccggcgacgg cacgttctcc gacgtcgagc tgatcaacat
     3901 cgccaacgcc ggggtcgccg cgttcggggt cctctacgtg ggcaacgcaa cgacctcccc
     3961 gatcgccaag accgtggtcg cggccctgtc cgcggtgctc acgctcctgg tcaacctcgt
     4021 cgcggacggc gtgaccttga gcgagtggct ccagctggtc gtcgcggccc tcggcgccct
     4081 cggtgtctac gccttcgcca accgcccggc caccaccagc ccggtcacgc ccagcgccta
     4141 gccgcacatc cggccacgac ctgacctgag aaggagggcc gtggtgtgac gctgcggcac
     4201 gacgtcaaga cgaggatcgg cgacagctgg gagtcgccca cgtgtggtcg aggaggtgcg
     4261 gcaccaccag ccgggggtcg ccgccgcggc ggtggtcggg gcgccggacg acgtgtgggg
     4321 cgagcgggtg gtcgccgtgg tcgagccggt ggccggccag gagccgaccg aggaggagat
     4381 ccgcgagttc tgccgacagc agctggccgg ctacaagtgc ccgcggcagg tggtgctcgt
     4441 gcccgagctg ccgcgcaaca cagtgggcaa ggtgctcaag agagagctgc gggagctgtc
     4501 tgcggtgacg ttgacaacac cctgacccgc tcgtagcgtc ttaacgaacg ttcagccaat
     4561 taatcgtttc cttcgacgaa gggctggaac atggtcagcg ctgaccccac gaccgccgag
     4621 ctgcgccttg tcgcgtccgg actgcagttc ccggagggcc ccatcgtgct gcgggacggg
     4681 cgcgtcctgc tcgtcgagat ccagcggggc accctgtcgc gggtcgcccc ggacggcgcg
     4741 gtctcggtcg ttgccgagct cggcggcggt cccaacggcg cggcgatcgg ccccgacggc
     4801 gccgcgtacg tctgcaacaa cggcggcttc gagtggcacg atcggggcgg gatcaccgcg
     4861 cccgggcacc agccggccga ctacatcggc gggcgcatcc agcgcgtggt cctcgacggg
     4921 ccggaggccg gaaccgtcac cgacctgtac accgagtgcg acggccggcc gctgcgtggc
     4981 cccaacgaca tcgtgttcga cgcccagggc gggttctggt tcacggacct cggcaaaagc
     5041 cgcgcccgcg acaacgacac cggctcgctc tactacgcac tgcccgacgg ttcgtcgatc
     5101 acggaggtgg tgcacccgct cgtacagccg aacggcgtgg gcctctcgcc ggacggctca
     5161 cgggtgtacg tggccgagac cggaccgggc cgggtctggt gctgggatgt cgcggggccc
     5221 gggcgcgtgc gcccggcgga cggggcgatg ggccctggcg gcgcggcgtt gctgcacggg
     5281 ttcgccggtt accagctgct cgactcgctc gccgtcgatg gcgagggcaa cgtctgcgtc
     5341 gccacgctgg tcaccggggc ggtcagcgtg atcgccccgg acggcacgct gctggaccag
     5401 ataccggtgc ccgagcccga cccgttcgtc accaacatct gcttcgcggg agccgactcg
     5461 cgcacggcgt acgtcacctc gtccggacgc gggttcctct acgtcacgca gtggcacacc
     5521 accggtctcg acctcgcgtt ctcggcatga ccacggctga cagcgcggtc agcgccgtcg
     5581 gcaggcgggc ggcgctcgcg gcccggttca cggcaccggc cgcggagttc gaggtcgtcg
     5641 agcggctcgt gcgggggatt cccacgcgcg tctactcggg cggcccgcag acgctgcgcg
     5701 agctcctgct cgccggcgcg gcgttcggcg agcgcgactt cctcgtgtac ggcacggagc
     5761 ggtggaccta cgcgcagcac ctgcggcagg tggccgggct cgcccgctgc ctgctggagg
     5821 agtacggcct gcgtaagggc gaccgggtcg ccgtcacgat gcgcaaccac ccggagtggg
     5881 ccccgatctt ctacgccacg caggtggcgg ggctcgtgct ggtgccgctc aacgcgtggt
     5941 ggaccgcgcc cgagctgcgg tacgcgctcc tcgactcagg tgcgcggttc gtcgtggccg
     6001 acgcggagcg cactgcgctc atcgcgccgc acctcgacga gctcggtggc atcccgctcg
     6061 tggaggtgcg cggcggggac aagccggcgc cgggggctcg gcggtgggcc gacctgctgg
     6121 cgtcgctcga cccggacgcg gccgtcccgg acgtcgacgt ggctcccgac gacgacgcga
     6181 cgatcctcta cacctcgggc accaccggcc ggccgaaggg cgcgatcggg acgcaccgca
     6241 accactgcac gaacatccgc aacacccagc tcggggcctt catcggcgcc acgatcgcga
     6301 acggcggcga gccgccggtg cccgatccgg acgcgccgca gccggggagc ctgtgcacct
     6361 tcccgctctt ccacatcgcc gggatcacca gcatctgcta ctcggtgctg gtcggcgcga
     6421 agctcgccac gcagtacaag tgggaccggg tcgaggccct cgagctggtg cggcgcgagc
     6481 ggctcaccgg ggtgtcgggg gtgcccaccg tcatgcgcca gctcgtcgag gacgccacgg
     6541 ccgatccggg gcgggtcccg acgctcgccg cgatcagcat gggcggcacg cccatcccgc
     6601 ccgacctgat cggcaggatc gacagcggct tctcctcgct ggtggcaccg gccaacggct
     6661 acgggctgac cgagacgacc tcggcggtcg tctccaacag cggcgccgac tacgtgacgc
     6721 accccgacag cgtcgggcgc tgcgcgccgg gggccgatgt gcgggtggtc gaccccgtca
     6781 atggggcgga cctgcccgac ggcgaggtcg gggagctgtg gttccgcggg ccgaacatcg
     6841 tccgcgggta ctggaacaac ccggaggcca ctgcggcggc cttcaccgac ggctggttcc
     6901 acaccggtga tctcggccgg gtccaggacg ggtggatcta cgtcgtcgac cgcatgaagg
     6961 acgtggtgat ccggggcggg gagaacatct actgcgccga ggtcgaggcc gcgctgttcg
     7021 agcacccggc cgtcggggac gtggcgatcg tcggggtgcc gcacgggtcg ctcggcgagg
     7081 aggccgtggc cgtcgtgcac ctgcgtccgg gtacgcagcc cggcccggct acggcagatg
     7141 acctgcgccg gcatgtcggg acgcggctcg cgtcgttcaa ggtgcccgcg cacgtcgtgt
     7201 tccgcgacga gccgttgccg cgcacgccca gtgggaaggt gctcaagcgc gacctgcgcg
     7261 gccccgtcgc ggcggagctg ggggtggcgc gctagatgca gccggatgtc aggtacgacg
     7321 tcgacgggca cgtcggggtc gtgactctcg accggccgca cgtgcacaac gcgctgcgcc
     7381 ggcagaccta cgacgagctg accgagctcg tgcgcaccac ctccgcccgg gtgctggtga
     7441 tcaccggtgc gggttcgtcg ttctgctccg gcgacgacgt ccgggagctg atgaacggcg
     7501 gcgagggcag ttcgccgcgt cccgcgcccc ggctcactcc cgcggccggc gcgttgctgc
     7561 tctccgacgt cccggtgatc gccgccgtga acggcccggc cgtcggctgg ggcatggaga
     7621 tggcgctgat ggccgacttc cgcatcgcgg ggcggcgggc gcggttcggc gagctgttcg
     7681 tcaagcgcgg cctgtgcagc gacgtcgccg gtatcgcccg gctcgcccag ctcgtggggc
     7741 gggaacgtgc tgcggagctg ttgttcaccg gcgaggtgat cgacgccgag cgggccgagc
     7801 ggatcggcct cgtcggccgg gtcgtcgacg acgaacagct gctcccggcg gccctcgagc
     7861 tggccggccg catcgcggcg aacccaccgc tggcggtcgc ggcgctcaag cgcgggctgc
     7921 ggcgcgcgct cgatccggac tggaccgacc tcgggatgtg ggtgagcagc acgctcggtg
     7981 agctgttcac cactgaggac catcgcgagg gcgtccggtc gttcctggag aagcgcgagc
     8041 cccagtacgt aggcaggtga gcatgtcggt gactgagtcg cggctcgccg gcatcgatgc
     8101 ggtccggccg gggcaggacc tacgcgctgc gtcgaccacc catgggcggc atgttgagca
     8161 ccgcgcacga catggatcga gagtggcggt tcatctcggg gctcgcagtg acggaagtcc
     8221 cggtggcgcc accgctggcc ttctgtgccg acaccgatgt caccggggcc gagttctacg
     8281 tcatgggctt cgtcgacggg ctcgtcctcg ccgaccgggt cgcggtcgtg tcgctgaccc
     8341 gcgcggctcg cgtccgggcg ggggagcacc tggtcgaggt gctcgtcgcg ctgcacgcgc
     8401 tcgacatacg aggtcgggct gggcgagatg gtgcgctcca ccgggtacgt cgagcggcag
     8461 ctgcggcgct ggcaccgtca ggtgcaccag tcgggcgccg acgacctgat tctgctcgac
     8521 gaggtgcacg acctgctcgc gcacacgtcc ctgcgtaggg cacggggatc gtgcacggcg
     8581 actaccggcc ggggaacatg gccttcgcac ctgacggcgc ggtgcgggcg gtgtacgact
     8641 gggagctggc gaccctcggc gaccccggcc gacacctcgg gcgccgcggg tgcggcgaag
     8701 cgcccctctg ctgggaactg ctgctccggt gaacctgtct caagcgatct tgacagagag
     8761 gggatgtccg ggaagcggac ggccctgcag gtgcccagaa ctcgggccca cgttgtcagg
     8821 atcgcgggac ggcacatgac tgccaacgcc tcggcatcga ggtcgaggtg ctgcggacgc
     8881 ggaggcctcc cctcctgctc ggcgacgacg attccgggtg tcagccggca gccactacca
     8941 gtgcaccgtc ggagacggcg gtgtcgagca cggtcgccgc gatgtgctca cggtgcgccg
     9001 ccgcgctgcc cagcagggct gcgtcggtga cagagcgctt gacgtacagg tgtgtgggat
     9061 gttcccaggt gaagccgatg ccgccgtgca gctgcaccgt gtccagggcc acccgctggt
     9121 aggcctcgga gcaggtggcc tgtgcgatgc tgacggccag gtctgggtcg tcgcctgcgc
     9181 tgccgtccga ggcgtcctgc agcgtccaca ccgcgtggta ggccgtggag cgggcgtgct
     9241 cgacgagcac gagcatgtcg gcacaccggt gcttgacggc ctggaaggat ccgatcgggc
     9301 ggccgaactg caggcgggtg cccgcatagg cgacggtgag gtcgaccagc ttctgcgccc
     9361 cgcctacctg ctcggtggcg agcagtgccg cgccgacccg gaacgcgtgc gtgatggcgc
     9421 gctcggcgcc ggcgccggtg gcgagcaggc gcccctccgc ggcgtgcagc cggaccgtgg
     9481 cctggcgccg ggtcgagtcc aacgtggaca acggaatccg ctcaccgccc tcgacggcga
     9541 acaggccgac gccggagccg gtgcgcgcgg ccacgagcag cacgtccgcg ctgccgtcga
     9601 cgacctggcg gacctcaccg gtcagcgccc cgtccgactc cgtgacggtg accagacccg
     9661 cgtcgaagcg tccggccctg tcgggcaccg cgagcgccgc ggtccgggtg ccctccacca
     9721 gtgcagtgag caggtcgtcg gcaccgccca gcgaaaccaa ggctgggatc gccagcgaga
     9781 cggtgccgaa caccgggccc ggcgtcagcg ccgccccgaa ctcctcggcg acgacagctt
     9841 ggtcgacgag cgtgccgccc acgccaccca atgcctcggg cacggcgagt cccagcaccc
     9901 cgagctcacc gcccaggcgc gaccagatgg ccgggtcgta gccgatgtct gtgtccatca
     9961 atcgccggac ggccgcctca tccgagcgtt cggcaaggaa ctgtcgtacc cctcgccgca
    10021 ggtcctgctg ctcctcggag aacacgaact caaccacggg ggatgtcctt ccacgccttg
    10081 ccggaatctg ctcgaaggtc gcccggaagc ccgaggatcc gctcgcccag gatgttgcgc
    10141 agcacctcgg acgtgccgcc ctcgatggtg ttggcccgcg agcgaaggaa ccgctgctgg
    10201 acgggacctc tgtagtcctc cccgcgcttg acgtcgtgca gttcgtagga gccgtacagc
    10261 gcggcctcag gcccgagcag ctccatgcag aactcgtaga cgtgctggtt gagctccgcg
    10321 gacacgagct tggcgatcga gccctccggg ccgggcgagc cgctagtggc cgcgcggccc
    10381 gcgcgggcgg aggtgagccg ctgcgcctcc gcccgcgtcc acagcgcggc gagcttgtcg
    10441 cgcaacaccg cggtgtgcag ctctggtcgt cgcgcccaca gctccacggc gtcggcgatg
    10501 gcacccgcac cccgcaccga cgagccaccg ccgatcgcgg aacgctcgtt catcagcgtg
    10561 gtcatcgcga cgcgccagcc gttgcccacg gcacccagcc ggtgcgtgtc ggggatgcgg
    10621 gcgtccgata gatagacctc gttgaactcg gcctgccccg tcatctgccg caaaggccgt
    10681 gtctcgacgc ccggtgcctg catgtcgagc acgaagtacg tcaatcctct gtgcttgggc
    10741 tgttcgggat cggtccgggc cagcagcagg gcgtaacggg ccaggtgcgc gaggctggtc
    10801 cagaccttct ggccgttgac cacccactct tccccatcct ggacggctga cgtcgctagt
    10861 ccggctaggt cggagcccgc accgggctca ctgaacagct ggcaccagat gtgttcggtg
    10921 gttgccagcg ggcgcaacag ctcggccgcc agcccaggct gggcgtgctc cagtagcgtg
    10981 ggcgcagcca tgccctggcc aatcggattg agagaaaaag cgataggccc gccggcgcct
    11041 tgcagcacct cgtcggcatg ggcctgtagc ccgcgcgaga ccccgagccc accaagcccg
    11101 acggggaagt gcacccacgc cagccccgcg tcgtagaccg cgccgaggaa ctcgcgcggc
    11161 ggggtcgatt gcgggtcgtg cgtctccact acctcccgag cggtcttctc gatcagctcg
    11221 ctgtgatcgt ccatgtcgcc tgatcccttc ctcgccgccg tcgccgagac agtcagggct
    11281 gaccgtatgg tatctgggcg ggactcggcc aggtctgctg cccgtttcac cagaccctca
    11341 gcggcaacaa caccaccgac agctcatgca gctgcaactg ggtccgccat gcggggccgg
    11401 ccgtttgtcg ggctcggcct ccacatcgac gtgagcgaac gtctcgcggc tcctgcaccc
    11461 cccgcgaagc gggcttcgac tcggtcctca tccctctcct cgccgcccag ttcgcccaca
    11521 tgcgcagact tcggcaggcc gtcggcggcc ggaacgcctc tcgacgcaga atgacgacaa
    11581 gtgcgagacc gcgttcttcc tcaaccccat cgggcacggc atggcggcgc ccacggtgct
    11641 cgagcacggg tcaccgcagc tggccaggga gctgctgcac ccacttgctc gaccgctacc
    11701 tgaacgatgc ccgcatcccc gatgcgcacc ggctcggcgc cgtccgcaac gggtggcgcg
    11761 tcgcgatgac caccagggtt actccggctc gccctggcag gcctcgaggt gccgggtgag
    11821 cgcccggacc aggccctcct cgctgaacgc gatgacgctc gctgaggcgc accaggggca
    11881 ggtgcggctc atgctcggcg ttggggagct gtccatctga tgaactttac ggattggccg
    11941 gccgcggggt cacgtgacgt aggcgagcgt cacaccggcg ctcaggacag cgcggcgctc
    12001 catcggcact gccgagacac ccgtcgacgg taactccggt gctccctcgc gcgatgagcg
    12061 ggcatgtcca cagtcactcc ggaccgcgtc gcggagccgg gtccctgcct caccaccgcg
    12121 ccgtggggtg gccggctcat cagcctgctg ttcaccgcga ccgtcctgct gttcctgctc
    12181 tggctgttcg cgctctgatc ccggcgcggg tgtgagcccg gcctcggact gaggtgtaga
    12241 cgggaagcgg gtcgtagtaa gtaccggtga acatcaacac gcaggcacga gtaccactcg
    12301 ggcgggtgct cgccctcggc ggcctgtcgt cgttcggtcc gctcgcgctc gacctgaatc
    12361 tgccggccct gccgcagcta gccgaggacc tgcgggcgcc ggtggcgtac gcgcagctgt
    12421 cgctgtcggt gtgcatgatc ggcctggcgc tgggccagct gctggtcggc ccgctggccg
    12481 accgcgtggg ccggcggcgc ccgctcctga tcggagtcgc atcgttcgcg gtcacggcgg
    12541 ggctgtgcgc ggtggccccg tcgatcgagg cactgctcgt cctgcggctg ctcggtggac
    12601 tggcgggcgg cgccgggctg gtgatcgcca gggccatggt tcgcgacctc tacggcggcg
    12661 cggcggcggc gcgggtgttc gcgctgctca tgctggtcag cggcacggct ccgatcgtcg
    12721 gtcccgtgct gggcgggcag ctgctgcggg tgaccgactg gcgcggggtg ttcgctgctc
    12781 tcgcgctgat cggcgcggtg ctgttcgtgg cggcgctgac gcagcgcgag acgctgccgg
    12841 cagcggatcg gcgcgcgggt gggctgcgtg cgacggccgc ggcgctgggc gacgtgctcg
    12901 tggaccggac gttcctgatg ccggcgctcg tgcagggcct ggggatgtgc gcgatgttca
    12961 cctacatcgc gatgggcagc ttcgtcctgc aggacggtcg ggcgctcgac ggggcgctga
    13021 acccgcagac ctacgccgtc gtgttcgcgg tgaacgggct cgggatcgtg ctcgccggac
    13081 gggtgagcgc agtcgtcgtc ggccgggtgg ggccgcgccg gctgctcggt gtgggcgtgg
    13141 tgctcgcgct cgtcgcggcg ctggcgctgc tcgccggggc gctcgcctcg cggtcggtgt
    13201 gggcgctgct ggtgccgttg ctgctgctgg tgtcgtgcac cgggctgttc atgccgaacg
    13261 cgaccgcact cgcgctggcc ggccagggcg gccgcgccgg caccgcctcg gcgctgctcg
    13321 gcctgctgca gttcaccttc gccgcggctg tgccgccgct ggcgtcgctg cgtggggtaa
    13381 cggcgacggt gatggccgtg acgatcctcg cgacggcgct ggccgccgcg ctggtgttcc
    13441 tcgcggtgct caggaccccg gtgccggccc cggtgggttg atcccgtcca ctgcgcggag
    13501 ccgctgccgc aggtgtgcgt gctgtgcagc gttctgcggc agcgcgaggg cggcggcgta
    13561 cgcggcccga gcctcgtcgg tccgcgcgag cctggcgaga agctcggccc gggcggcgtg
    13621 ccacagggcg tactcctcga gcccggtcac cgcatcgacc tcggcgagcc ccgcaccagg
    13681 tccctgctgt tccccgacgg caaccgcccg gttgagccgc acgaccgccg tgtcctgcac
    13741 ggtcagcagg acgtcgtacc aggacaggac ggcggaccag tccgtgtcgg gataggacgg
    13801 ggcgagcgcg tggcacgccg cgatcgccgc ctgcacgaca taggggtcgg gccggccggg
    13861 ggagcgccgc aggccctcgc cgaccagcac gaccccctcc tccacgaggt cgtgccgcca
    13921 cagcccgcgg tcctggtcgg ccagcagcac gggacggccg tcccgggcgt agcgcgcgtc
    13981 ccggcgggag tcctgcaaca gctccagcgc cagcaaaccc aggaccgacg gttcgcccgg
    14041 catcagctcc cgcagcagcc gggtgagccg cacggcctcg tcgacgagcg cgggccgcag
    14101 cgggtcgtcc ccggccgttg ccgcgtatcc ctcgttgaac agcaggtaca cggtggcgac
    14161 aacgccgcgg agccggtcgg gaagctcgtg gtcctcgggc acccggtacg ggatggcggc
    14221 gcgggcgatc ttctgcttcg cacgggtgag gcgcttgttc atggtcgctt cgggtagcaa
    14281 cagtgcgcgt gccacctccg ccgtggagag cccgcagagc gtccgcagtg ccagcgccac
    14341 ctgcgtctcg acagccaggc tcgggtggca gcaggtgaac accagccgca ggaggtcgtc
    14401 gcgcagcacc tcggggggct cgggcgtcga cggttcgggt gggaccacgg tgccggcctc
    14461 cttcccgtcc cgcgcggctt cccggcggat gagatcgatc gcccggcggc gggcagtgag
    14521 ggtgagccac gcccgcggct cctccgggac gccgtcgcgc gaccaggtct ccagcgcacg
    14581 cacggtggcg tcctgcaccg cgtcctccgc gagctggagg tcgccggtga gccgcaccag
    14641 ggtggccagc acgcgccggc cctcgtcgcg cacgaggcgg acgagggcgg cgcgcgcgag
    14701 atcgtcgccg cgggcggtcg atccggccat tcagcgctgc atcgggggga tcaccggccg
    14761 gacctcgatc gctccgtccc gcgcgcccgg aatcctcgcg gccgtggcga cggcctcgtc
    14821 gaggtcggcg cactcgaaca ggtagaagcc ggtgagcgcc tccttcgtct ccgcgtacgg
    14881 cccgtcggtg gtgacgacgt cgccgccctt gccgccgtgc acccggacgg tggtggcggt
    14941 gctggtcggg tagagcgccg cgccaccgcg gatcaccgcc gcagcggcgc gcccgaactc
    15001 gccgtactcg gccatcgtct cggagtgctc ggccgccgac cagtcgacgt cggcgttgta
    15061 gatcagagcg atgtagctgg gcatgtgtgc tcctcggggt gcgtccggcc ggccctgtcg
    15121 gccggcgtca ccctagggac gaacggcgcg gccgcggaag gacacaccta ctctcatcga
    15181 atgcggatcg cctcgttgct gccggccgcc accgagatgg tcgcggcgct gggccgggcg
    15241 gacgacctgg tcgctgtgac gttcgagtgc gacgagccgg ccggcgtccg ggagcgggcc
    15301 gtcgtggttg tcgacaccgt cctgccgccg ggcctgaccg ccgccgcgat cgacgccgtg
    15361 gtccgcgacc ggagcgcgcg cggcctgccc ctgtacgagc tggaccgcga cgcactcgct
    15421 gcgtcgtccc cggacctcct catcacccag gacctgtgcc gcgtgtgcgc actccccgcc
    15481 ggtgccgtgg acgaggcgct ggcggcgatc gggtgcgacg cggcggtgct ctcgatcgac
    15541 ccgcacagtg tggacgaggt gttcgccgcg atcaccgcgg tcggccacca tatcgaggcc
    15601 ccggaagcgg ccgcggcgct ggtcggctcg ctgcgcgacc gcctgggagc ggtgcacgac
    15661 gccgtggccg ggctgccgcg gccgcgcgtg ctggtgctgg agtgggtcga cccgcccttc
    15721 ctgcccggac actgggtgcc ggagctggtt cggttcgccg gcggcgaacc cgtgggcggt
    15781 gccgacgggg gccgctcggt gcaggcgagc tgggacgcaa tcgccgcgct tcccagcgac
    15841 gtcgtggtgg tcgcgccgtg cgggttcggc ctcggtgcgg cggcggaaca ggccgcgacc
    15901 gtacgcgacc ggtttcccgg ccgcccggtg gtggcgatcg actccgcgtc ctacgtcgtg
    15961 cgggccggac cgcggctcgt ggacggcgtc gaggcactgg cctggtcgct gcacccggag
    16021 gcggtccggg agccacctcc ggcacgggtg gcggtactgc ggtgagcctc ggcaggttca
    16081 gacaggatca gcgcccggct gcgggggtag cgcacggggg ggtggggtaa cccgaagcgc
    16141 gaccgacccg accctgggag gaagagatga ccggagccac cgccgacctc gacgtgatcg
    16201 acgagctgat gtccgaccac cgtgaggtga cctcgctgct cgaccgtatc gacaccacca
    16261 ccgaccccgc cgacaagcgc gagctcgccg acatcgcgat caccgagctc gtccggcacg
    16321 ccgtcgcgga ggagatgtac gtctacccgg cgatgcgcga gaacctgccc gacggtgagg
    16381 aggtggtgaa gcacgacacg gaggagcaca aggaactgga gcggacgatg aaggagctcg
    16441 agggcgtgga ggcgacggat ccgcgcttcg agcagctcgt cggcgagctg cgccgcgagc
    16501 tccagcacca tgtggaggaa gaggagagcg agcagtttcc caagttccgg cagcgcgttc
    16561 cgcacgacga gcgggtccag ttgcgggaga aggtcgagaa ggcgaagaag ctggccccga
    16621 cccgtccgca ccccaatgcc cccaaaagcc cgctgttcca caaggtcgtc ggacccggcg
    16681 tcgggctcgt cgaccgcgtg cgcgacaagc tcacgggccg cgcctgaggt gccgtccgtc
    16741 gtcaggagcc gggcggggcg gccgggcgta gcatcctggc cgtgaccggc cccgctgacc
    16801 tcggagaggt ccgcgcgcac accgtgctcg ccgacgccga cggcactccg tactcgtcgc
    16861 tgcggcagcg gcccgtggct cgggccgagc gttatgccct cggcaaggcg ctgcgcaagc
    16921 gggtgccgag gtcgtccctc ggcgagtgga agccgccgtc ggaccggccc gacccggtgc
    16981 agctgatcag ggagagccac cgcggccggg tggagcggct ggtgcccatc cgggtcggtc
    17041 gcatggtcgc ctcgccctac ggtttcctcc gcggcactgc cgtcgtcatg gccgaggacg
    17101 tcgcgcagct cccgtccacc ggaatcaccc cggtcgtgtg cggcgacgcg cacctgggca
    17161 acttcgtttt ctacgcctcg ccggagcgcg acctcgtgat cgacctcaac gacttcgacg
    17221 aggcccaccc gggtggctgg gagtgggacc tgcgccgcct cgtcgcgagc atctgggtcg
    17281 ccggacggca caacggcgcg agcgaggacg gctgcgcggc cgccgtgtcg tcctgcgtgt
    17341 cggcctaccg cgaggagctg cgccatcttg ccgaccagcc gctgatgtca cgctcctacg
    17401 agcgccttga cgtcgaccgg ctcgagcagg agacggcggg accgttgcgc gccgaggtcg
    17461 aacgggccgc ccggcgggcc cggcagcgca cgagcgaccg cgcgctgccg cgcttcaccc
    17521 agcaggtcgg gggccgccgc cggatcgtcg aggagccgcc gctgatcacc cgggtgtccg
    17581 acagcgacgc ggagctgctc ggcgtcgcgc tcgacgggta cctgcaaacg ctcgcgccgc
    17641 actggcgcag ggtgctgggc ggctacacgc tggtcgacat cgcgcacaag gtggtcggcg
    17701 tcggcagtgt ggggttgcgt gcctatgtcg ccctgctgga gggctcctcg ccggaggacg
    17761 tggtgttcct gcagctcaag caggcccgcc ggtcagtgct ggcgcggtac gtgcacggcg
    17821 agtcggcgtg gcaccgccac caggggcagc gggtggtgga gtaccaacag gccctgcaga
    17881 ccgtcagcga cccgctgctg ggctgggcga ccgtctcggg tcgtgacgac gacgtgcacc
    17941 agtactacgt ccgccagttc aggaacatga agggcacggt cgcgctcgac gcgatcgacg
    18001 ccgccgcgct cgccgactac gcaggggtcg tcgggcacct gctcgcgaaa ggccacgccc
    18061 ggaccagcgg cgcgtcgatg atcgccggtt acgtcggggg ctcggccaag gtcgacgacg
    18121 cgttgtgcgg gttcgcccgc gcctatgccg accagacgga ggccgaccac accgcgctcg
    18181 tcgcggccgt tgctcggggc ctgctgccgg tcgagcgcgg ggtataggag tgcgccgcac
    18241 gcgtgggcga tctagcggcg ggcgcggcga tgccaggtca gtgcccacac accgagcagg
    18301 acgatcagga tcagcaccat gctcgccgcg ccggtggttc cgccggcgag cgcggcgagc
    18361 agcacgagca gggcggccgc catcgcggtt cctgcgacga cggcgcgccg gggcgaacca
    18421 ggcgggacgc cctgccgcag tgcctcggcc aactggggat cgtcgttgcc gagctgtcgt
    18481 tcgagttcgt ccagccggat ccgctccgat gcggtcaggc ggcggccgtg aggttgatct
    18541 gcgagcatgc cggggagtac ccagagaacg caccgcttca cgtgtcgggg gtgtcggatc
    18601 gggatcggcg gaaggacgac cgggtggcca tcgaactgac cgcggcgaga ccgagggatc
    18661 gcctccggcg ttcggtccgg cggctcacgc gggcgctggg tccggccatg gggctgagca
    18721 cgcccgcgct gatgcgccac ccggtgtgga cgacgccggc cgtggagggc ggcggcacgg
    18781 gcgtggtggt cgttcccggg ttcggaggcg tggacgcgag catggtggtg ttgcgccact
    18841 ggctccggcg acgtggctac gtgccggtcg gagcacgact cggggtgaac ctggggtgca
    18901 cggacgcgct ggtcgcccgg ctcgagcggc gcgtggagga gctcgtccgg agaaccggcg
    18961 gcccggtggt cctactgggg cacagccgtg gcgggatgct cggcaggctc gtggcggtgc
    19021 ggcgccccga cctggtgtgc gggctcgcca tgctcggcag cccggtgctc gaccccctcg
    19081 acgcccgcgg gatggcgcag cacctcctgc gggtgctggt gcggctgtcc gcgttcggcg
    19141 tgcgtggcct gctcgagcag gactgcctca cgggtgcttg ccgggacacc acggtcgagg
    19201 gtctcgtcgc gccgctgcgg gtgccggccg tcgccatcta ttcccgggag gacggggtgg
    19261 tcggctggcg gtcgtgtcgc gacccggagg ccgagtggtt cgaggtcacg agcagtcaca
    19321 ccgggatggg caccgacccc gtgctctatg acgcgctcgc gacccggctg gcccggtggg
    19381 tggcggaccg tgccggcggg cctggagatc acgggccgag ctcgtgcacc cgccccgagt
    19441 cggtggtgtc gtagccgccc agctccgtga tcgtcgcgcg cacggccggg tcgtgcagcg
    19501 tcgccacgag cggtgcggcg gcgtccagcg cggccgcacc gaggacgacg tcgtagtcct
    19561 cgtccaccag cgacacgaag tccaggtcca ggtcgcgtgc caccgagcgg acgcccagcc
    19621 ccgcgtcgac cacaccggac gccaccgtga gccccgtctc caggtgcgat cccgtctccg
    19681 ggccggccac cgcgtccggg tccaggtccg cggcgagcag cagccggtcg aacagcaccc
    19741 gtgtgcccga gccctgcctt ctcttggcca cccgcagccc cgccaggtcc gcggccgtcg
    19801 tcagcccgcg tgggttgccc ggcggcacga cgatgccctg ttcgcggcgc cacaggtgga
    19861 tcaggtgcgg tcgccaccca cgcagcaggc cacgcgcgaa cggggcgttg tagacgccgg
    19921 tgtggtgcag caggtgcacc gcggctccgt cggccttccc ctggcgaacg gcacgcaggc
    19981 cgccgaagct gccctcggcg ccgatggcct gcaccgcacg gccgagggcg cgtgtcacga
    20041 cgtcgagcgc ggggtcgtcg ctgccggcca gccggaacac cagctcggag tgcaggaacc
    20101 ggcgcgcggc cacggcgccg tcgggcgcca ctctggagcg ccgccggtcg gaggtgagga
    20161 tcaccccggc gtcggccagc gctcgctggg cccggctcac cgtggaggcc gttgtgccga
    20221 accgcgaggc gagctcgcgc actccgggta gctcggatcc ctcggtgagc tcacctgatc
    20281 gaatccgtcc ggccaagtcg gagcagatct cccggtatcg cgtcacggct cagcacccta
    20341 ttcttccgga ctgtgctacc ccagatgaga tcagtggtcg ccgccctctc gttgttactg
    20401 ctggtcgcct gtgggggccc ggcggcggcg ccgtcggccg agcggtcgct gatcctggcc
    20461 accaccacca gcacccagga ctccggcctg ctcgacgatc tgctccccgc gttcacagcc
    20521 gacaccggct ggcaggtgaa gacggtcgcg gtgggcagcg gtcaggccat cgaactgggc
    20581 cgccgcggag aggccgatgt gctgctggtg cactcgcccg ccgccgagga gaagttcgtc
    20641 gccgagggaa cggccgggcg ccgcctgctc gtcatgcaca acgacttcgt gctcctcggc
    20701 cccgcggccg acccggcggg aatccgcggc accggcgtgg ccgacgcgat gacccggatc
    20761 gcgaactccg gggcggtctt cgtgtcccgc ggcgatgact ccggtaccgc cgcacgcgag
    20821 aaggcgctct ggcagcaggc gggagtgatc ccgggcggcg ggtggtacca ggagaccggc
    20881 cagggcatgg gcgcgaccct gagggtggcc gcggagaagg gcggctacac cttgtccgac
    20941 cgcgccacgt acctcagcca gtccgacggg ctgactctgc tgaacgaggg cgaccccggc
    21001 ctgctcaacg tctaccacgt gatcgagatg acgacgaagg ccggcgatcg ggtgcagccc
    21061 gacgccgccg ccgcgttcgc cgactggatc accgggccgg ctgcgcagca gcggatcggt
    21121 gagttcggcc gcgcgcagtt cgcgcagccg ctgttcgtgc ccgacgcggg caagcctgaa
    21181 ccggtgccct gatggatgtg ctgctcgacg gtttcgtgca ggccgtgcag ctgttgctgg
    21241 gtggtgacgc cgccacgtgg gcgatcaccg gcctgaccct gcggatctcg ctgaccgcca
    21301 ccgtgatcgc cgccatcgtc ggggtgccac tgggcgccgc ggtggcgctc acgtcgttcc
    21361 gtggccggcg tgtagtgctg gccgcggcca acaccggcat ggggctgccg cccgtggtgg
    21421 tcgggttgtt cgtcaccgtg ctgctgtggc gcagcggccc gctcggggcg ttcgggctgc
    21481 tctacaccca gacggccatg atcatcgcgc aggcggtgat cgccacgccg atcgtggtgg
    21541 cgctcgtggc agctgcggtg cagcagatgg atccggactt cctggtgcag atgcaggggc
    21601 tcggcgccac gcggaccagg gcactggctg cgctgctcgg tgaggcgcgg ctgccgttgc
    21661 tcgcagccgc gatggccgcg ttcggcgcgg tggtcagcga agtcggcgcg gcgcagatgg
    21721 tgggtgggaa cctcgcgggg gagacccggg tgctgaccac cgcggcggtg ctcgccacca
    21781 gccgcggcca gttcgcgctg gccatcgcgt tcgggttgat cctgctgatg atcgcattcg
    21841 cggtgaacct ggtgctcacg ctggtcgggc agcgccaggc ggtcccctcc cgatgacgtt
    21901 ccggatcggg tgccgggacc tgcgcgtggt cgtgcgtggc cgggagctgc tgcacgtcga
    21961 ccggctcgac gtggccgcgg gggcggtgct cgccgtgttg ggcccgaacg gggcaggcaa
    22021 gtcgacgctg ctgcgggcgc tggcgcggct gggccggcac cacgtcagcg gcgaggtgct
    22081 gctcgacggg agccccgccg ggccgcggga gatgcgcccc gcggtggcgg cagtgctgca
    22141 gcggccgatc ctgcgccgtg ggacggtgac cgccaacgcc gccgcggggc tgcgcctgcg
    22201 cggcgtcggc cggtccgacg ccgtccgccg gtccgtgccg tggctcgatg
//
LOCUS       DASRSC010000046        18593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_14376,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000046 DASRSC010000000
VERSION     DASRSC010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 18593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18593
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14376"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VFE39_02255"
     CDS             complement(<1..190)
                     /locus_tag="VFE39_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257450.1"
                     /translation="MTVTAPFTPNAEPTGALPTGLLIGGRVVEGRAAPIQVTNPATGE
                     VFAEVASASEEDIDEAVRV"
     gene            351..1025
                     /locus_tag="VFE39_02260"
     CDS             351..1025
                     /locus_tag="VFE39_02260"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6257451.1"
                     /translation="MAAAESAAGRVTRDLRRRVLSGELKPGTVLSQSRIAAEYDVSRM
                     PARDALQVLANEGLVDLGPATSVVRELSIAELQELYELREAIEPVLTRLAVPNVGRAQ
                     IAQMTSLVDVMETAPSAADWMDANARFHEHVYTSVDRPRMIELTEHLRRLTDRYLYLH
                     VGVFGDVDHLHDEHRQILAAVRAGDAAGAAELTRIHLATSHEFILRYLLEHALLLPLA
                     HGSETR"
     gene            complement(1070..2506)
                     /locus_tag="VFE39_02265"
     CDS             complement(1070..2506)
                     /locus_tag="VFE39_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6257452.1"
                     /translation="MTTTESSAPAATAVTPYDELAAALRGDLITPGDPGYDEARSVYN
                     AMIDKRPAAVARCRDVADVMTCVRFGRDHDLEIAVRGGGHNAGGLGVWDDALVIDLSL
                     LRSTTVDPENHTVRADAGCTWGDVDHATVAFGMATPSGIIASTGVGGLSLGGGIGYLA
                     RRFGLTVDNLLAADVVLADGTFVTASESSHSDLYWALRGGGGNFGVVTSFTFRCHDIG
                     EGGTIIGGPVLYDFADTAAVMRWYRELLPSLPEELNGWIGLITIPPAPPFPEELWGRR
                     SCAIVWCYTGPHDKADDVLEPIRTFGAPLLVGLHDMPYTVLQGAFDDLYPAGLQWYWR
                     ADFFDEITDAAIDVHRMYGEQLPSGHSTMHLYPIDGAASRVPDDATAFAYRDGGWAGV
                     IVGVDPDPANAGAISRWARDYWEELHPTSAGGAYVNFLMDEGQDRVRASYRGNYERLA
                     QVKKRYDPDNTFHINQNIRPARGSMPSA"
     gene            complement(2559..5768)
                     /locus_tag="VFE39_02270"
     CDS             complement(2559..5768)
                     /locus_tag="VFE39_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6257453.1"
                     /translation="MLHISLLGEQSISDDATGTVRTRSSRSLALVAYLVVHAGSPQSR
                     QRIAGLFWPDSTDAQALTNLRRELHHLRLVLDDRPPLVVTSRDLCWQDAPTCRVDVRT
                     FDVERRAALAAAAAGETDGVITHAGAATAQYRGDLLPGVYDDWLLEARSELESQCVDL
                     CDLSRDARARNGDLAGAVDAARRRIHLRPLEEAGYRSLMELQADLGDRAGAVSTYHHC
                     ASVLERELGLDPDRTTRAALQRLMARTDPATTPPLPTIDVPAARTGPAAAKLVGRSRE
                     MQVLHTLWATAAAGRPIIALVRGDAGVGKTRLVDEVAELARRQGAVVASTQCFGTSER
                     LALAPVADWLRNPAVRASMATLDPVWRVEVDRLVPSGDGRGEPGASLRAMVDAWQRHR
                     FYEGLARALLGGGRPMLLVLDNVQWCDQETLAFLTFALGLASDRPVMVAATRRDENLD
                     DEPGPADWTLRMRATGKLTELLLSPLDVADTARLAEAISGQPLLDEDANLLQAATGGF
                     PLYVVEAARANVHLGSADLGGADLGSADLLGAPLPVSDLTAVLRNRLDQVTPAAREVA
                     GLAAAVGRNFTLDLLSEASDLDAGDVVHAVDELWRRRIIREFRDGYDFSHDLLRDTAY
                     AQISPPKRWLLHRRLAQGLELLHSDDPDGVSAQLAEQYARGGRAERAVAYYRRAADVA
                     AGRFANAEAIRLHREALSILRTLPEGRDRDGQELGVLEAMAAPVNARYGYSSPRLQDT
                     LERSITLAERLGRKNSVLGGLVGLWASRFVQGRIADAHRTASRLLTMVEPDSELSGSA
                     HFAFGGSAVSLGMPEEALHHFEVAAELTRGAPSLRVGTRPAVHVRAWAAHAHWLLGHD
                     AEALSSCRDAIGQARAINHPYSLAVALAYGGITHQIRHELPELGDTVAELRELCDRYD
                     FAYYREWGLVLDGWARTDGSGSAVARRGIENLRAEGSFARMPYWLSLLAECSTREDRA
                     AAARATLDAALIAGQARDDLWWMPQVMRMRAAYDDPGAAVARLRSAAQMSRTHGSVAL
                     LRRCERDLAERGVRPPAEDVLGVPRSANAATNAAGTPGS"
     gene            6192..6605
                     /locus_tag="VFE39_02275"
     CDS             6192..6605
                     /locus_tag="VFE39_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HET6257454.1"
                     /translation="MEPFTRTGTYLGVLATAVAVAAASACAEPSAGPGTPPPAEHQMS
                     MPAPAAAASVQTVAVSAVAISGFAYVPAAITVPAGTVVTWRNNDSVRHDVSGGPLRSP
                     LLSQGATWSYTFATPGTYSYICSVHPNMTGTVTVR"
     gene            complement(6657..7679)
                     /locus_tag="VFE39_02280"
     CDS             complement(6657..7679)
                     /locus_tag="VFE39_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET6257455.1"
                     /translation="MVRTIRFHETGGPEVLRYEDVPVRTPGPGEVRIRVDAIGLNRAE
                     VNFRRGTYLDRPVLPAGLGTEASGEILEVGPGVERWSEGDAVSVVPAFSQNDYPVYAE
                     EAVVPAAALVARPDGMDAVTGAAIWMPYVTVYGMVSEIVRVRPGDRVLITAAANSIGM
                     AALQVVRHLGAVPIATTPIADQRPALLAAGAAGVIITGAHDDPDGASDLPAAVHACTD
                     GHGADVVLDAVGGPQVGQLVQACAPRATVIVHGGLSGLPTPLPGGGYAPVWLRRYRVF
                     EITTDPAALRRAEHFVRSGLATGAFAPVVDRIFDFDDIVAAHRYVDSPERGPGKPVVR
                     LQHRAR"
     gene            complement(7709..7882)
                     /locus_tag="VFE39_02285"
     CDS             complement(7709..7882)
                     /locus_tag="VFE39_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257456.1"
                     /translation="MADQSVSEEAREAVLKAIIKSAPTVGNSPAGIRDLAEAYALLER
                     DLLPNAGVVHNKT"
     gene            complement(7923..8951)
                     /locus_tag="VFE39_02290"
     CDS             complement(7923..8951)
                     /locus_tag="VFE39_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET6257457.1"
                     /translation="MTLPAVVLGLMFVGLIAYALFAGADFGAGIWDLLAGGTRRGARQ
                     RDLIEHSIGPVWEANHVWLIFVLVVLWTAFAGAFAALATTLYIPLTLAAFGMIARGAA
                     FAFRKSITTLGMRRFLGAAFAVSSLITPFFLGAVVGGVASGRVPPGIAAGDVVTSWVN
                     PTSILGGVLAVLVCAYLAAVFLCADARREGPEDLAEQFRARALGTAAVTGAVGLGGFF
                     VLRADAPLLFAGLTGRGLPVIVLSVVAGLVAIALLVLRRYASSRLASGLAVTTILIGW
                     AVAQYPYVLPPYLTIDQAAAPRTTLVATLVALIVGGAILVPALAYLYTLFQSEHAEHA
                     EVGSPQPR"
     gene            complement(8948..10357)
                     /locus_tag="VFE39_02295"
     CDS             complement(8948..10357)
                     /locus_tag="VFE39_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET6257458.1"
                     /translation="MEQIELVLSDAAENLLPARMQMAVSLGWHIIFVCFGLALPVIVV
                     FTEWRAHRRDDPALHELARAWAKAMGVLFAAGAVSGTLLSFELGILWPGLMGRFGPVF
                     GFPFVLEGFAFFIEAIFVGVYLFGWDRMSPRAHILSGIPMIVSALAGAFFVISANAWM
                     NNPTGFTMDAQGRVVDAHPVAAMFGPSTWPQWAHMYTAGFMVTGFTVASVYAVGMLRG
                     RRDRYHQIGMLIPLTIASIAAPVQIVIGDWIAGNVARNQPVKLAAMEGLYRTGTGVPL
                     SVGGIYYDDRLHYSLEIPWGLSLLVTRNPNGEVVGLEAVAPDLRPPVNVVHLAYDSMV
                     AMGSALLALAAWLGYMWWRHRRAPRTVWFLRAVAVSGVAAIIALEGGWITTEVGRQPW
                     IVYNILRTADAVSPAPGLYLGFYAMLAIYLVLTVLTVYVLRRLARTHGTAAPQEGTAA
                     QQEIDAPPSERPGQEDAVR"
     gene            10727..11026
                     /locus_tag="VFE39_02300"
     CDS             10727..11026
                     /locus_tag="VFE39_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HET6257459.1"
                     /translation="MVGVVLIAAQALVAYPERRVEAPEVSLGVVYMPGVPVVSTVWGL
                     SLGAANAVLGIAAFDLFHVQPACSSYAPIPQAPVAFVTCLSAALLLGLVAIASGW"
     gene            11126..12208
                     /locus_tag="VFE39_02305"
     CDS             11126..12208
                     /locus_tag="VFE39_02305"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6257460.1"
                     /translation="MFAAVVEEVGRLLPVDLASMCRYESDGSLTWVAAWGRAGEHFPV
                     GTRRKLGGNNLGTIVLETGRSARIDNYADSCSGAIGAVAREAGINSSLATPIMVEGRL
                     WGVIAAGSIQKQPVPADTEERLASFTELVATAIANAESRAALAASRARIVAAADAARR
                     RIERDLHDGTQQHLVSLMLELRLAESSVPAELEEARSRISRVAGELNRVIDDLREISC
                     GIHPAILSEGGLGPALRTLARRSAIPVELTSVIKHRLPEPIEVAAYYVVSEALTNAAK
                     HANASQVNMEASARDGSLRLSIRDDGVGGAVPAGGSGSGLVGIRDRVEALGGSIEIIS
                     PAGHGTHVVVQFPLELDLAIDSPLKT"
     gene            complement(12274..14352)
                     /locus_tag="VFE39_02310"
     CDS             complement(12274..14352)
                     /locus_tag="VFE39_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET6257461.1"
                     /translation="MDVPQDRPGSGPFDELFERLIGNLETTLGSAMPRPDSATGAPGT
                     RTSGTRPRTPRLDRFGRDLTAAAAQGRLDPVIARDAEIEQVLEVLARRTKNNPVLVGD
                     PGVGKTAIVEGIAQRVAAGTVPETLKDVRVVSLDLAGMVAGTRYRGDFEQRLTGVIDE
                     VVAAQRSIVLFVDELHAVVGAGAAEGGAMDAGTLLKPALARGDLQLIGATTLQEYRRH
                     IEKDAALERRFEPVRVAEPSVAATIEILRGLRGRYEEHHDVRISDAAIVAAAELSDRY
                     LTDRFLPDKAIDLIDRASARARIKAGRPDVDPPVDERLEQLRRARDVAVDAEDFERAH
                     MLTREIDAAETGPGTPAPAPAGAGPVEITRDDVAAVLAQSTGIPLARLTVTERHRLLD
                     LESLLHRRVVGQDDAVEAVADAVRAGRAGLAHPDRPIGSFLFLGPTGVGKTELARALA
                     EALFGSTDTLLRFDMSEYSDHGSAMRLVGAPPGHVGYDDAGQLTEAVRRTRYAVLLLD
                     EIEKAHPDVIATLLQVLDAGRLTDAHGRTVDFTNTVVIMTSNLGADRLLAAAEAGRPV
                     EEVRVPVLAAMRAHFRPEFLNRIDDIVLFHALDRAELRRITHLLVAETGDRLRAQGIT
                     LDLTESAVDWLAERGHQPELGARPLRRTIARELDRKLSRLIIAGDLGPGQRIVVDAAD
                     GRLVLEPRKN"
     gene            14480..15517
                     /locus_tag="VFE39_02315"
     CDS             14480..15517
                     /locus_tag="VFE39_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015102477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6257462.1"
                     /translation="MPRSVSDQVVVIAGASSGIGRASALAFAARGAKVVCAARTARAL
                     DTLVEQITSTGGSAVSVPTDVAEPGAVEALAAAAEERFGRIDTWVNAAAVGVWGRVED
                     ISSEEFDRVMRVNFLGHVHGVHAALPALRRAGGGGIIGISSLEGVRSVPLQGPYTASK
                     WALRALYDTLRMELAAEGEPIAVSTILPAAIDTPFFEHARSKVGAMPKPPPPVYAPEV
                     VADTIVYTAEHPRREVAVGGVAVGFVAGQRLSPALTDAVMSIPRLGVDEMTADRPDNG
                     VDNLDAPIDEAGQVHGNYSGTVLRRSTVTDLLRRVPRPGELLTGALSSIRRARSNGSG
                     TPAVTPAPPPG"
     gene            complement(15569..17686)
                     /gene="glgX"
                     /locus_tag="VFE39_02320"
     CDS             complement(15569..17686)
                     /gene="glgX"
                     /locus_tag="VFE39_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET6257463.1"
                     /translation="MEIWPGDAYPLGATYNGAGTNFALFSELAHRVELCLFDDEDIEH
                     RVELSEVDGFIWHCYLPDVEPGQRYGFRVHGPFDPGAGLRCNPAKLLLDPYGKSFDGP
                     VDWDESLFDYRWDRPELRNDDDSATHQTRSVVVSPFFDWGNDRPPRTRYSESVIYEAH
                     VRGLTVLHPEIPPALRGTYAGLAHPAVVEHLQQLGVTAVELMPVHQFLDDHNLVQRGL
                     ANYWGYNTVGYFAPHRRYAARGGRGQQVTEFKAMVRTLHEAGIEVILDVVYNHTSEGN
                     HLGPTLAFRGIDNPAYYRLADDPRYYADYTGTGNTLNVRHPHTLQLIMDSLRYWVLDM
                     HVDGFRFDLAAALARGLYEVDRLAAFFDLVQQDPVVSQVKLIAEPWDLGAGGYQVGNF
                     PPLWTEWNGRYRDSVRDFWCGRPGTVGEFASRLTGSSDLYESSGRRPYSSINFVTAHD
                     GFTLHDLVSYNGKHNAANGEDNRDGTHDNRSWNCGVEGPTDDPEIEELRERQKRNLLT
                     TLFLSQGVPMLLHGDELGRTQGGNNNAYCQDNRISWVDWKRAEGFRSLTDFTTRLARL
                     RREHPVFRRRRFFQGRPVRGTSLEDLAWLTPGGLPMGDAQWTDGNTRALAIFYNGDGI
                     PEPDRHGRKLRDDSFLLLVNPTPEVVAFTLPGPAYGTGWAVVLDTADPQRGAHGAPGA
                     PAARARATLAVPGHSQQLLVRVP"
     gene            complement(17747..18085)
                     /locus_tag="VFE39_02325"
     CDS             complement(17747..18085)
                     /locus_tag="VFE39_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257464.1"
                     /translation="MTEQAIELDLDETGVARDLPQPVHQRDEVQQVPYRPVQFRDDDL
                     PTALERSAAWLRRAQEWLGEPLDVIAVHLDYDDSDTGPYYDVKLLCNEEDLAGAPIAV
                     RAQQAQVATN"
     gene            18223..18483
                     /locus_tag="VFE39_02330"
     CDS             18223..18483
                     /locus_tag="VFE39_02330"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HET6257465.1"
                     /translation="MAGPAVAFVRATGAALDGRQRDSVGATLSELATLLREMGVDDAV
                     NLDGGGSSTLVYREPGPDPGRIVNDPSASPRLVPNGGGVYTT"
BASE COUNT         2688 a         6427 c         6775 g         2703 t
ORIGIN      
        1 cgacgcggac tgcctcgtcg atgtcctcct cgctcgcgct cgccacctcc gcgaacacct
       61 cgccggtcgc cgggttcgtg acctggatcg gcgcggctcg gccctcgacg acgcggccgc
      121 cgatgagtaa tccggtcggc agggcaccgg tgggctcggc gttcggagtg aacggggcag
      181 tgaccgtcat gtacaaactc ccctcgtctc gtatacacat cactagaatc ggtatacgac
      241 ttgacgcttg tcggtgccaa ccgcgtgaca aagtgcacag agacgggctg gcttcaccat
      301 gagcaggcgg catgcatact ggccgtgacg ggcgaaggca gggaggtcag atggctgcgg
      361 ccgaatcggc ggcggggcgg gtcacccgtg atctccgccg tcgcgtgctc agcggcgagc
      421 tgaagccggg caccgtgctg tcccagtcgc ggatcgcggc ggagtacgac gtgagccgca
      481 tgccggcccg cgacgcgctg caggtcctgg ccaacgaggg cctggtggac ctggggccgg
      541 ccacctccgt ggtgcgcgag ctgtcgatcg ccgagctgca ggagctctac gagctgcggg
      601 aagccatcga gccggtgctc acccggctgg ccgtgcccaa cgtcggccgc gcccagatcg
      661 cgcagatgac gtcgctcgtc gacgtgatgg agacggcgcc ctcagcggcc gactggatgg
      721 acgccaacgc gcggttccac gagcacgtat acaccagcgt cgaccggcca cggatgatcg
      781 aactgaccga gcacctcagg cggttgacgg atcgttacct gtacctgcac gtcggggtgt
      841 tcggcgacgt cgaccacctg catgacgagc accgtcagat cctcgccgcg gtccgtgccg
      901 gtgacgcggc aggcgccgcc gagctgacgc gcattcacct cgcgacgtca catgagttca
      961 tcctgcgcta cctgctggag cacgcgctgc tgttgccgct cgcgcacggc agcgagacgc
     1021 ggtgacaccg ccccgatccg cgcgagcagg tcggggcggc tgatccgggt caggccgacg
     1081 gcatcgaccc gcgggcgggg cggatgttct ggttgatgtg gaacgtgttg tccgggtcgt
     1141 agcgcttctt gacctgggcc agccgttcgt agttgccgcg gtaggacgcc ctgacccggt
     1201 cctgtccttc gtccatcagg aagttgacgt aggcgccgcc tgccgaggtc gggtgcagct
     1261 cctcccagta gtcgcgggcc cagcgcgaga tggcacccgc gttcgccggg tccggatcga
     1321 cccccacgat gacccctgcc cagccgccgt cccggtaggc gaaggctgtc gcgtcgtccg
     1381 gaacacggga ggctgcgcca tcgatcgggt acaggtgcat ggtggagtgg ccggagggca
     1441 gctgctcgcc gtacatgcgg tgcacgtcga tggcagcgtc ggtgatctcg tcgaagaagt
     1501 ctgcccgcca gtaccactgc aggcctgccg ggtacaggtc gtcgaaggcg ccctgcagca
     1561 cggtgtacgg catgtcgtgc aggccgacca gcaatggggc gccgaacgtt ctgatcggct
     1621 cgagcacgtc gtcggccttg tcgtgcggtc cggtgtagca ccagacgatg gcgcaggacc
     1681 tgcggcccca gagctcttcg gggaagggcg gggcgggcgg gatggtgatc agtccgatcc
     1741 agccgttcag ctcctccggc agtgacggga gcagctcccg gtaccagcgc atcaccgcgg
     1801 ccgtgtcggc gaagtcgtag agcaccggac cgccgatgat ggtgccgcct tccccgatgt
     1861 cgtggcagcg gaacgtgaag gacgtgacga cgccgaagtt cccgccgcca ccgcgcaggg
     1921 cccagtagag gtcgctgtgt gagctttcgc tggccgtcac gaacgtgccg tcggcgagca
     1981 ccacatcggc ggccaggagg ttgtccaccg tgagcccgaa gcgccgggcg aggtagccga
     2041 tacctccgcc gagcgatagg ccgcccacac ccgtggacgc gatgatgccc gacggggtgg
     2101 ccatgccgaa ggcgacggtc gcgtggtcga cgtcgcccca ggtgcatcct gcgtcagcgc
     2161 gcaccgtgtg gttctcgggg tcgaccgtgg tgctgcgcag cagcgagagg tcgatgacca
     2221 gggcgtcgtc ccacacaccg agcccgcccg cgttgtggcc gccaccccga acggcgatct
     2281 cgaggtcgtg gtcacggccg aaacggacac aggtcatgac gtcggcgacg tcgcggcatc
     2341 gagcgacggc cgcaggacgc ttgtcgatca tcgcgttgta gacggagcgc gcctcgtcgt
     2401 acccggggtc tcccggcgtg atcaggtcgc cgcgcagggc tgctgccagc tcgtcgtagg
     2461 gagtgacggc ggtggccgct ggtgcgctcg attcggtggt ggtcatgagg gtccccttgg
     2521 ctgtggcgct cgacgtccga gcgatccacc cgccgaagct aggagcccgg cgttcccgca
     2581 gcgttcgtcg cagcgttcgc agatcgaggg acgccgagga cgtcctcggc aggaggacgg
     2641 acaccccgct cggcgaggtc acgctcgcag cgccggagca gcgccacaga tccatgggtc
     2701 cgcgacatct gcgccgccga ccgcaaccgt gcgaccgcgg cgcccgggtc gtcgtaggcc
     2761 gccctcatcc gcatcacctg cggcatccac cacaggtcgt cccgagcctg tccggcgatg
     2821 agcgctgcgt cgagggtcgc ccgggcggca gcggcccggt cctcccgtgt cgagcactcg
     2881 gcgagcagcg acaaccagta cggcatgcgg gcgaacgagc cctccgccct gaggttctcg
     2941 atgccccgtc gggcgacggc ggagcccgac ccgtcggtgc gtgcccaccc gtcgaggacc
     3001 aacccccact cgcggtagta cgcgaagtcg taccggtcgc agagctcgcg cagctccgcg
     3061 acggtgtccc ccagctcggg cagctcgtgc cgtatctggt gggtgatacc gccgtaggcc
     3121 agtgcgacag cgaggctgta cgggtggttg atcgcccggg cctgcccgat ggcgtcacga
     3181 cagctggaca gagcctcggc gtcgtggccc aggagccagt gggcgtgcgc cgcccaggct
     3241 ctgacgtgca cagcaggccg ggtgccgacc ctcagggatg gcgcacctct cgtcagctcg
     3301 gcggcgacct cgaagtgatg cagtgcctcc tccggcatac cgaggctcac ggccgatccc
     3361 ccgaaagcga agtgggccga gccgctcagc tcggagtcgg gttcgaccat ggtgagcaag
     3421 cgggaggccg tgcggtgtgc gtcggcgatg cgcccttgga cgaaccgcga cgcccacaac
     3481 ccgacgaggc cccccagaac ggagttcttg cggccgagcc gctcagcgag ggtgatgctg
     3541 cgctccaggg tgtcctgcag cctgggggag gagtagccgt agcgggcgtt gaccggcgct
     3601 gccatggctt ccaggacgcc cagttcctgc ccgtcccggt ccctgccttc cggcagtgtg
     3661 cgcaggatcg acagcgcctc tcgatgcagg cggatcgctt cggcattggc gaacctgccc
     3721 gctgcgacgt cggccgcgcg ccggtagtag gccacggccc gctccgctcg tccgccgcgg
     3781 gcgtactgct ccgcgagctg tgccgagaca ccgtccgggt catcggagtg gagcagctcg
     3841 agcccctggg cgaggcgccg gtgcagcaac cagcgcttcg gtgggctgat ctgagcgtag
     3901 gcggtatcgc gcagcaggtc gtgggagaag tcgtagccgt cccggaactc gcggatgatc
     3961 ctgcgccgcc acagctcgtc gacggcgtgc accacgtcgc ccgcgtcgag gtcgctcgcc
     4021 tcgctgagca ggtcgagggt gaagttccgt ccgacggcgg ccgccagccc cgcgacctca
     4081 cgagcggccg gcgtcacctg gtcgagccgg ttgcgcagca ccgcggtgag gtcgctgacc
     4141 ggcagcggcg cccccaggag atcagcgctg ccgagatcag cgccgccaag atcagcgctg
     4201 ccgaggtgga cgttcgcccg tgccgcctcg acgacgtaca gcgggaaccc gcccgtggcc
     4261 gcctgcagga ggttcgcgtc ctcgtcgagg aggggctgcc ccgagatcgc ctcggcgagg
     4321 cgggcggtgt cggcgacgtc cagcgggctg agcaggagct cggtgagctt tccggtggcc
     4381 cgcatccgga gggtccagtc agcgggtccg ggttcgtcgt cgaggttttc gtcccgtcgg
     4441 gtggccgcca ccatgaccgg tcggtcggag gccagcccga gggcgaacgt gaggaacgcg
     4501 agcgtctcct ggtcgcacca ctgcacgttg tcgaggacca ggagcatcgg acgcccgccg
     4561 ccgagcagag cccgcgccag gccctcgtag aagcggtggc gctgccaggc gtcgaccatg
     4621 gctcgcaggc tggcccctgg ctctccgcga ccgtcgccgg aaggcaccag cctgtcgacc
     4681 tcgacgcgcc acaccgggtc cagcgtcgcc atcgacgccc ggactgccgg gttgcgaagc
     4741 cagtcggcca ccggcgccag cgccagccgc tccgaggtcc cgaagcactg ggtgctcgcc
     4801 accaccgcgc cctggcgccg ggccagctcg gcgacctcgt cgacgaggcg ggtcttcccg
     4861 acgccggcat cgccgcgaac gagcgcgatg atcgggcggc cggctgcggc ggtggcccac
     4921 agggtgtgga ggacctgcat ctcccgggac cgtccgacga gcttcgctgc ggcgggcccg
     4981 gtacgggccg cggggacgtc gatcgtcggc agtggaggtg ttgttgccgg gtcggtgcgg
     5041 gccatcaggc gctggagtgc cgcgcgcgtc gtccggtccg ggtcgaggcc gagttcgcgc
     5101 tccaggaccg aagcgcagtg gtggtaggtg ctcaccgccc cggctcggtc gcccaggtcg
     5161 gcctgcagct ccatcaggct ccggtagccg gcctcctcca acggtcggag gtggatgcgc
     5221 cgccgtgccg cgtcgacggc ccccgccagg tcaccgtttc gcgcccgcgc atcacggctc
     5281 aggtcgcaca ggtccacgca ctggctttcg agttcggacc tggcctcgag cagccagtcg
     5341 tcgtacacgc cgggcagcag gtcgccccgg tactgggcgg tggccgcgcc ggcgtgggtg
     5401 atcacgccgt cggtctcgcc tgccgccgcc gctgcgagcg cggccctgcg ttcgacatcg
     5461 aaggtgcgca catcgacccg gcaggtcggg gcgtcctgcc agcacaggtc cctggacgtc
     5521 acgacgagcg gaggccggtc gtccaggacg agtctcaggt ggtggagctc gcgacgcagg
     5581 ttggtcagtg cctgggcgtc ggtcgagtcg ggccagaaga gcccggcgat gcgctgccgg
     5641 gactggggag acccggcgtg gacgaccagg taggccacca gcgcaagcga ccgcgacgac
     5701 cgagtccgga cggtgccggt tgcatcgtcg gagatcgact gctccccgag gagagagatg
     5761 tgcagcatct cgcgcgccct cccgagccgt caccatagac ccggctgcgg cgtcgtcgct
     5821 cgcggttgtc ccggggcggt cccccgtcct ccactcggtg gtcgtactcg cggcggttgc
     5881 cgagtcgggc gaccggtggc tgagggcggg caggaccatt ggccctagat ctgggggatc
     5941 aggaacctcg acgatggggc ggcctcctcg aacgtggggg tgatcccgac gctcctgtgc
     6001 tggtccgccg ggtcgtatga cctgcgccct cggcccggcc gcgcgccggc gcagcgcggc
     6061 ggtagggcgg atacttggcg acccggtttg cgaaccgggt cacgcaggca ctgaggacag
     6121 cccagagccg atggccgccc agcaggagca gttcgccggt ttggggatcc gtcaccaccc
     6181 gggaggcacc tatggaaccg ttcacccgca ccggcaccta cctcggcgta ctggccacgg
     6241 ccgtcgcggt ggccgcggcc agcgcctgcg ccgagccgtc ggccggccca ggtacgccgc
     6301 cgccggcgga gcaccagatg tcgatgcccg cgcccgcagc cgctgcgtct gtccagaccg
     6361 tcgcggtcag cgccgtggca atctcgggct tcgcctacgt ccccgcggcc atcacggtcc
     6421 cggccggcac tgtggtcacc tggcggaaca acgactcggt ccggcacgac gtctccggcg
     6481 ggccgctgcg ctcgcccctt ctgagccagg gcgccacctg gagctacacc ttcgccaccc
     6541 cggggaccta cagctacatc tgctcggtcc acccgaacat gaccggcaca gtcacagtca
     6601 ggtgagcaac ggcggccgat tgcgcctacg aggatcgccg atgcggcgtt ggacgctcag
     6661 cgggcgcgat gttggagccg tacgacgggc ttgcccggac ctcgctcggg ggagtcgacg
     6721 tagcggtgcg cggcgacgat gtcatcgaag tcgaagatgc ggtccaccac cggcgcgaac
     6781 gcgccggtgg cgagcccgga gcggacgaag tgttcggcgc gacgcagcgc agccgggtcg
     6841 gtcgtgatct cgaagacgcg gtagcgccgc agccacaccg gcgcataccc gccgccgggc
     6901 agcggcgtcg gcaggccgga caggccgccg tgcacgatca cggtcgcgcg cggcgcgcag
     6961 gcctgcacca gctggcccac ctgtgggccg cccacggcgt cgaggaccac gtcggcgccg
     7021 tgcccgtcgg tgcaggcgtg gacggccgcc ggcaggtccg atgcgccgtc ggggtcgtcg
     7081 tgggcgccgg tgatgatgac gccggcggct ccggcggcga gcagggcggg gcgctggtcg
     7141 gcgatcggcg tggtggcgat ggggaccgcg cccaggtggc gcacgacctg cagcgcggcc
     7201 atgccgatgc tgttggctgc cgcggtgatc agaacgcggt cgccgggccg gacccggacg
     7261 atctccgaga ccatgccgta cacggtcacg tacggcatcc agatcgccgc tccggtgacc
     7321 gcgtccattc cgtcgggccg ggcgacgagc gccgccgccg ggacgacggc ctcctcggcg
     7381 tagacggggt agtcgttctg ggagaaggcc ggcacgacgc tcaccgcgtc gccctcggac
     7441 cagcgctcca cgccggggcc gacttcgagg atctccccgg acgcctcggt acccaggccg
     7501 gccgggagca ccggacggtc gaggtaggtg ccgcgccgga agttgacctc cgcccggttg
     7561 aggccgatcg cgtccactcg gatccgcacc tcacccggac ccggggtgcg gacgggtacg
     7621 tcctcgtagc gcagcacctc gggccccccg gtctcgtgga acctgatcgt ccgcaccatg
     7681 cacgcgaatc tacggctccc ggcagccctc aggtcttgtt gtgcaccaca ccagcgttcg
     7741 ggagcaggtc ccgttccagg agcgcgtacg cctcggcgag gtcgcgaatg cctgccgggc
     7801 tgttgcccac agtcggagca gacttgatga tcgccttgag cacggcctca cgggcctctt
     7861 cgctcacgct ctgatcggcc atgccggatg acgctacccg ggcgggggcg gctccgggga
     7921 ggtcagcgag gctgcgggga cccgacctcc gcatgttcgg cgtgctcgct ttggaacagc
     7981 gtgtacaggt acgccagcgc ggggacgagg atggctccgc cgacgatcaa ggccacgagc
     8041 gtggccacca gcgtggtccg aggtgccgct gcctggtcga tcgtcagata gggcggcagc
     8101 acgtacgggt actgggcgac cgcccacccg atcaggatgg tggtgacggc cagcccggac
     8161 gcgagccggg agctcgcgta gcggcgtagc accagcagcg cgatcgcgac gaggcccgcg
     8221 accacggaca gcacgatcac cggcaggccg cggccggtca gccccgcgaa caacagtggg
     8281 gcgtcggcgc gcagcacgaa gaaaccgccg agcccgaccg cgccggtcac cgcggcggtg
     8341 ccgagcgcac gggcgcggaa ctgttcggcc aggtcttcgg gtccttcgcg gcgggcgtcg
     8401 gcgcacagga acaccgcggc aaggtacgca cacaccagca ccgccagcac cccgcccagg
     8461 atcgaggtcg ggttcaccca gctggtgacg acgtcgcctg ccgcgattcc cggcggcacg
     8521 cgaccggagg cgacgccgcc gacgaccgcg ccgaggaaga acggcgtgat cagcgaggac
     8581 acggcgaacg cggccccgag gaatcggcgc atccccaatg tcgtgatgct cttgcggaac
     8641 gcgaaggcgg cgcctcgcgc gatcatgccg aacgcggcca gcgtcaaggg gatgtagagc
     8701 gtggtcgcga gcgcggcgaa cgccccggcg aacgcggtcc acaacaccac cagcacgaag
     8761 atcagccaga catggttggc ctcccagacc gggccgatcg agtgctcgat caggtcacgc
     8821 tgtcgggcgc cgcgccgggt accgcctgcc aggaggtccc agatcccggc cccgaagtcc
     8881 gcaccggcga acagcgcgta cgcgatgagc ccgacgaaca tcaggccgag caccacagcc
     8941 gggagcgtca tcggaccgca tcctcctggc cgggccgctc cgatggtggc gcatcgatct
     9001 cctgctgggc ggccgtgcct tcctgcgggg cggccgtgcc gtgggtgcgg gcgagccgac
     9061 gcagcacgta cacggtgagg acggtgagga ccaggtagat cgccagcatg gcgtagaagc
     9121 cgaggtacag gcccggtgcc gggctcacgg cgtcggccgt ccgcaatatg ttgtagacga
     9181 tccagggctg gcggcccacc tcggtggtga tccagccgcc ctccagcgcg atgatcgccg
     9241 cgaccccgga caccgcgacc gcgcgcagga accacaccgt gcgcggggct cgtcggtgcc
     9301 gccaccacat gtagcccagc cacgcggcga gcgcgagcag cgcggagccc attgcgacca
     9361 tggagtcgta ggcgaggtgc accacgttga ccggcgggcg caggtccggg gccacggcct
     9421 ccaggccgac gacctcgccg ttcgggttgc gggtgaccag cagcgacagg ccccacggga
     9481 tctccaggga gtagtgcagc cgatcgtcgt agtagatgcc gcccaccgac agcggtaccc
     9541 ccgttcccgt ccggtacagc ccctccatcg ccgccagctt gaccggctgg ttccgggcga
     9601 cgttcccggc gatccagtcc ccgatgacga tctggaccgg cgcggcgatc gaggcaatgg
     9661 tcagcgggat gagcatgccg atctggtggt aacggtcgcg gcggccgcgg agcatcccga
     9721 cggcgtacac cgacgcgacg gtgaacccgg tgaccatgaa cccggccgtg tacatgtgcg
     9781 cccactgcgg ccaggtggac ggcccgaaca tcgccgcgac cggatgggcg tcgaccaccc
     9841 ggccctgggc gtccatcgtg aagccggtcg ggttgttcat ccacgcgttg gcggagatga
     9901 cgaagaacgc cccggccagg gcggagacga tcatcggaat tccgctcagt atgtgcgccc
     9961 tcggcgacat gcggtcccag ccgaagaggt agaccccgac gaagatcgcc tcgatgaaga
    10021 aggcgaagcc ctccagcacg aacgggaagc cgaacaccgg gccgaagcgc cccatgaggc
    10081 cgggccacag gatgcccagt tcgaacgaca gcagggtgcc cgagaccgcg ccagccgcga
    10141 acagcacgcc catcgccttg gcccaggcgc gcgcgagctc gtgcagcgcc ggatcgtcgc
    10201 gccgatgggc gcgccattcg gtgaacacca cgatcaccgg aagcgcgagc ccgaagcaga
    10261 cgaagatgat gtgccagccg agcgacaccg ccatctgcat acgggcgggc aggaggttct
    10321 ccgcggcgtc ggagagcacg agctcgatct gttccacatc ggcctccact tccgtatcgg
    10381 gcgcgccatc ctcatgggag ttcccggaaa atcaggacgg gaacgggcac cggtcagctc
    10441 gcgcgggtgc gcgaccgacc ggccgtggcg ctccgcccga tgcgcagcaa gcgctggagc
    10501 gcatcggcca gttcgtcgag gagcgcacct cgggggccgt tgactttcgc gcgacgcgtt
    10561 cgggaggcgg gccgctgagg tcgttcagcg cgccgccgac cagtggcgcg gagatggctg
    10621 agcgagcggt ctgaatcgtg gcgaggcggg ggtacgcttg ctgcttgatg cgcgatcgtg
    10681 cgttgtcgcc gctgatcctg acgtccctcc gctggtcttg gggatcgtgg tcggcgtcgt
    10741 gttaatcgca gcgcaggcgc tcgtggcgta cccggagcgc cgggtcgagg ccccggaggt
    10801 ctcgctgggg gtggtgtaca tgccgggcgt gccggtggtc tcgacggtgt gggggctgtc
    10861 cctcggagcg gcgaatgcgg tgctcggcat cgccgcgttt gatctcttcc acgtccagcc
    10921 tgcttgcagt tcgtacgcgc cgattcctca ggcgcccgtg gcattcgtga cttgcctgtc
    10981 ggccgcgctg ctactcggct tggtcgccat cgcgagcgga tggtgagtgt ccttgagcga
    11041 tgagcagctg cgtgtgctgg ctgaggagca ggcggccttg cggcgggtcg cgacgctggt
    11101 ggcgcgtgga acgcggccgg aggaggtgtt cgctgcggtt gtcgaggagg tcgggcggct
    11161 gcttccggtc gatctggcga gcatgtgccg ctacgagtcc gacggttcgc tcacctgggt
    11221 cgcagcctgg ggaagggcag gcgagcactt tcccgttgga acccgacgga agctcggggg
    11281 gaacaacctc ggcacgatcg tgctcgagac cggccgttcc gcccggatcg acaactacgc
    11341 tgacagctgc tccggcgcga tcggtgccgt cgcccgcgaa gcgggcataa actcgtcgct
    11401 cgcgacgccg atcatggtcg agggccgcct ctggggcgtg atcgccgccg gctcgataca
    11461 gaagcagccg gtgccggcgg acaccgagga gcgcctcgcc tcgttcacgg aactggtggc
    11521 cacggcaatc gcgaatgcgg agagtcgcgc cgcgctcgcc gcctcacgcg cacgcatcgt
    11581 cgccgccgcc gatgccgcac gccggcgcat cgagcgtgac ctgcacgacg gcacccagca
    11641 gcacctggtc tcgctgatgc tcgagctgcg cctggccgag tcgagtgtgc ctgcggagct
    11701 cgaggaagcc cggagcagga tcagccgagt cgcgggcgag ctcaaccggg tcatcgacga
    11761 cctgcgggag atctcctgcg gcattcatcc ggccatcctc tccgagggag ggctcggacc
    11821 ggcgttgcgg acgcttgccc gccgttcggc gattccggtc gagctcacca gcgtcatcaa
    11881 gcatcggttg ccggagccga tcgaggttgc ggcctactac gtcgtctccg aagcgctcac
    11941 gaacgcggcc aagcacgcga acgcctccca ggtcaacatg gaggcttccg cacgagacgg
    12001 cagcctgcgg ctctcgattc gtgacgacgg cgtgggcggc gcggtgcctg cgggcggctc
    12061 gggctcgggc ctggtaggca tccgtgaccg cgtggaagcg ttgggcggat cgatcgagat
    12121 catcagcccg gccgggcacg ggactcacgt cgtggtccag tttcccctgg agctcgacct
    12181 ggccatcgac agcccgttga agacctgatc agccagaatg cgggccgatc gccgatcgcg
    12241 gccgcccacg ccggtccagg ggctctgcgc aggtcagttc ttccgcggct ccagcaccag
    12301 cctgccgtcg gccgcgtcga cgacgatccg ctgcccgggg ccgaggtcgc cggcgatgat
    12361 caggcgagag agcttgcggt cgagctcgcg tgcgatcgtg cgccggagcg gccgggcccc
    12421 gagctcgggc tggtggccgc gctcggcgag ccagtcgacc gcgctctcgg tgagatccag
    12481 cgtgatgccc tgggcacgta gccggtcgcc ggtctcggcg accagcagat gggtgatccg
    12541 gcgcagctcc gcgcggtcga gggcgtggaa cagcacgatg tcatcgatac ggttgaggaa
    12601 ctccggacgg aagtgggcgc gcatcgcggc gagcaccgga acgcgcacct cctcgacggg
    12661 ccgcccggcc tccgccgcgg caaggagccg gtcggcgccc aggttgctgg tcatgatcac
    12721 gaccgtgttc gtgaagtcga cggtgcggcc gtgggcatcg gtgaggcggc cggcgtcgag
    12781 cacctgcagc agggtggcga tgacgtcggg gtgggccttc tcgatctcgt cgagcagcag
    12841 caccgcgtac cgggtgcgcc ggacggcctc ggtgagctga ccggcgtcgt cgtatccgac
    12901 gtgcccaggg ggcgccccga ccagccgcat cgcgctgccg tggtcggagt actcgctcat
    12961 gtcgaagcgc agcagcgtgt ccgtcgaacc gaacagtgcc tccgccaggg ccctggccag
    13021 ctcggtcttg ccgacgccgg tcgggcccag gaagaggaac gagccgatcg ggcggtcggg
    13081 atgcgccagg ccggcccgtc cggcgcgcac ggcgtcggcg acggcctcga cggcgtcgtc
    13141 ctgcccgacg acgcggcggt gcagcaacga ctccaggtcg agcagccggt gccgttccgt
    13201 gacggtcagc cgcgcgagcg ggataccggt gctctgtgcg aggacagcgg cgacgtcgtc
    13261 ccgggtgatc tcgacgggac cggcaccggc cggggcaggg gcaggggtgc ccggccccgt
    13321 ctcggccgca tcgatctccc gggtgagcat gtgggcgcgc tcgaagtcct ctgcgtcgac
    13381 ggcgacgtcg cgggcgcggc gcagttgctc caggcgctcg tccaccggcg ggtcgacgtc
    13441 cgggcggccg gccttgatcc gggcgcgcgc gctcgcccgg tcaatgaggt cgatggcctt
    13501 gtcgggcagg aaccggtcgg tgaggtaccg gtccgacagc tcggcggccg cgacgatcgc
    13561 agcgtcgctg atccggacgt cgtgatgctc ctcgtagcgg ccgcgcaggc cgcgcaggat
    13621 ctcgatcgtc gcggcgaccg agggctcggc tacccggacc ggctcgaacc gccgctccag
    13681 cgccgcgtcc ttctcgatgt gacggcggta ctcctgcagc gtcgtcgcgc cgatgagctg
    13741 caggtcaccg cgggcgagtg ccggcttgag cagggtgccg gcgtccatcg cgccgccctc
    13801 ggccgccccg gcgcccacca cggcgtgcag ctcgtcgacg aacagcacga tcgaccgctg
    13861 cgccgccacg acctcgtcga tgacgcccgt gagccgctgc tcgaagtcgc cgcggtaccg
    13921 ggtgcccgcg accatgcccg ccaggtccag cgagaccacc cgcacgtcct tcagcgtctc
    13981 gggcacggtg cctgccgcca cccgctgggc gatgccctcg acgatcgccg tcttgccgac
    14041 gccggggtcg ccgaccagca ccgggttgtt cttggtccgg cgggccagca cctcgagaac
    14101 ctgctcgatc tcggcgtcac gggcgatgac cgggtcgagc ctgccctgcg cggccgcggc
    14161 cgtcaggtcc cgcccgaacc ggtccagccg aggggtgcgc ggacgggtcc ccgatgtcct
    14221 cgtgcccggt gcccccgtgg cgctgtcggg ccggggcatg gcactgccca gcgttgtctc
    14281 caggttcccg atgagacgtt cgaacagctc gtcgaagggt ccggatccgg ggcggtcctg
    14341 cggcacgtcc acctccacga gggtgcatcc gttgtcgatg gtcctccgtc caggcccggt
    14401 ccgcccagtg gcgagctccg attggggtgg ttgagccggg gtagtccgac gccgcaaccg
    14461 gggcgaggga gtggatgaga tgccgagatc agtgtcggac caggtggtcg tcatcgcggg
    14521 cgcctcgagc ggtatcgggc gggcgagcgc cctcgcgttc gccgcgcgcg gtgcgaaggt
    14581 ggtctgcgcg gcccgcactg cgcgggccct cgacactctc gtggagcaga tcacgagtac
    14641 aggcggctcg gcggtctccg tcccgaccga tgtcgcggaa cccggcgcgg tggaggcgct
    14701 cgccgcggca gccgaggagc gtttcggccg gatcgacacc tgggtcaacg ccgcggccgt
    14761 cggtgtgtgg ggccgcgtcg aggacatcag ctccgaggag ttcgaccggg tgatgcgggt
    14821 caactttctc ggccacgttc acggggtgca cgccgccctg ccggccctgc gccgtgcggg
    14881 cggcggcggg atcatcggga tctcgtcgct ggagggcgtg cggtccgtgc cgctgcaggg
    14941 cccgtacacg gcgagcaagt gggcgttgcg cgccctctac gacacgctgc ggatggagct
    15001 cgccgcggag ggcgagccga ttgcggtgag caccatcctg ccggcggcga tcgacacgcc
    15061 gttcttcgag cacgcccgca gcaaggtcgg ggccatgccg aaacccccgc cacccgtgta
    15121 tgccccggag gtcgtcgccg acacgatcgt ctacacggcc gagcatcccc ggcgcgaggt
    15181 ggccgtcgga ggcgtcgccg tcggcttcgt cgccgggcag cggctctccc cggcgctgac
    15241 ggacgcggtg atgtcgatcc cgcgcctcgg cgtcgacgag atgacggccg accgcccgga
    15301 caacggggtg gacaacctcg atgcgccgat cgacgaggcg ggccaggtgc acggcaacta
    15361 ctcggggacg gtgctgcgtc gcagcacggt caccgacctg ctcaggcgtg tgccccggcc
    15421 cggcgagctc ctcaccgggg cgctgtcgtc gatccggcgc gcccggtcga acggcagcgg
    15481 cacgcccgcc gtgacaccgg cgccaccgcc gggctgagcg accaggatcg gctgtcagcg
    15541 gcccctggag ccgaggaagc gcggctcctc acgggacgcg gaccaggagt tgctgggagt
    15601 gcccggggac cgcgagggtg gcccgggccc gtgcggcagg cgccccgggc gcgccgtggg
    15661 ccccgcgctg cgggtcggcc gtgtcgagga ccacggccca cccggtgccg tatgcggggc
    15721 cgggcagcgt gaaggcgacg acctccgggg tcgggttgac cagcaacagg aacgagtcgt
    15781 cgcgcagctt ccggccgtgc cggtccggct cggggatccc gtcgccgttg tagaagatgg
    15841 cgagtgcgcg cgtgttgccg tcggtccact gcgcgtcgcc catcggcaga ccgcccgggg
    15901 tgagccaggc gaggtcctcc aggctcgtcc ccctgaccgg cctgccctgg aagaaccgac
    15961 ggcgccggaa taccgggtgc tcgcggcgca gccgggcgag gcgggtggtg aagtcggtga
    16021 gggaccggaa gccctcggct cttttccagt ccacccagga gatccggttg tcctggcagt
    16081 aggcgttgtt gttgccgccc tgcgtgcgcc ccaactcgtc accgtgcagc agcatcggga
    16141 cgccctgcga gaggaacagg gtggtcaaca ggttgcgctt ctgccgttcg cgcagttcct
    16201 cgatctccgg gtcgtcggtg ggaccctcga cgccgcagtt ccacgaccgg ttgtcgtgcg
    16261 tgccgtcccg gttgtcctcg ccgttcgccg cgttgtgctt gccgttgtag gagaccaggt
    16321 cgtggagtgt gaagccgtcg tgggcggtga cgaagttgat cgaggagtac ggccgccgac
    16381 cgctcgactc gtacaggtcc gaggaccccg tcagccggga ggcgaactcc ccgaccgtgc
    16441 ccggccggcc gcaccagaag tcgcgcaccg agtcccgata gcggccgttc cactccgtcc
    16501 acagcggggg gaagttgccg acctggtagc cgcccgcccc gaggtcccac ggctcggcga
    16561 tgagcttcac ctgcgacacc accgggtcct gctggaccag gtcgaagaag gcagcgagcc
    16621 ggtcgacctc gtagagccct ctggccagcg cggcggccag gtcgaaccgg aacccgtcga
    16681 cgtgcatgtc cagcacccag taccgcagcg agtccatgat cagctgcagg gtgtgcgggt
    16741 ggcggacgtt cagcgtgttg ccggtgcccg tgtagtcggc gtagtagcgg gggtcgtcgg
    16801 ccagccggta gtaggcgggg ttgtcgatgc cgcggaacgc cagcgtcgga ccgaggtggt
    16861 tgccctccga cgtgtggttg tagacgacgt cgaggatcac ctcgatgccc gcctcgtgca
    16921 gggtgcgcac catcgccttg aactcggtga cctgctgccc gcgcccaccc cgtgccgcgt
    16981 agcggcggtg cggggcgaag tagccgacgg tgttgtaccc ccagtagttg gccagcccgc
    17041 gctgcacgag gttgtggtcg tcgaggaact ggtgcaccgg catcagctcc accgcggtca
    17101 cgcccagctg ctggaggtgc tcgacgaccg ctggatgtgc caacccggcg taggtgcccc
    17161 gcagcgcggg cggtatctcg ggatggagca ccgtcagtcc gcgcacgtgc gcctcgtaga
    17221 tcaccgactc gctgtagcgc gtgcgcggcg gccggtcgtt gccccagtcg aagaaggggc
    17281 tgacgaccac cgaccgggtc tggtgggtgg ctgagtcatc gtcgttgcgg agctcgggcc
    17341 gatcccaccg gtagtcgaag agtgactcgt cccaatccac cgggccgtcg aacgacttgc
    17401 cgtagggatc cagcaggagc ttcgcggggt tgcagcgcag gcccgcaccg ggatcgaacg
    17461 ggccgtgcac ccggaacccg taccgctggc cgggctccac atccggcagg tagcagtgcc
    17521 agatgaagcc gtcgacctcc gacagctcca cccggtgctc gatgtcctca tcgtcgaaca
    17581 ggcagagctc gacgcgatgg gccagttcgg agaagagggc gaagttcgtg cccgcgccgt
    17641 tgtaggtggc gcccagggga tacgcgtccc ctggccagat ctccacaaga ccatcctgca
    17701 ccggcgcggg cggcgacgct gccgtccacc ccggtgatcg tcagggtcag ttcgtcgcga
    17761 cctgcgcctg ctgcgcgcgg acggcgatgg gcgcgccggc cagatcctcc tcgttgcaga
    17821 ggagcttgac gtcgtagtag gggccggtgt cgctgtcgtc gtagtccagg tgcacggcga
    17881 tgacgtccag cggctcgccc agccactcct gggcccggcg cagccaggcc gccgatcgct
    17941 ccagggctgt cggcaggtcg tcgtcgcgga actgcacggg ccggtagggc acctgctgca
    18001 cctcgtcccg ctggtgcacg ggctgcggga gatccctcgc cacgccggtt tcgtcgagat
    18061 cgagttcgat cgcttgctcg gtcacagact ccagggtggc ctggcaggga ccctcgcggc
    18121 aagcggaccg tccgcgacac gcccatggcg cggacggtcc gtgcctgccg ttctcgctcc
    18181 tgcttctgct gagcgtctcc agccggatca ccgattggcg cggtggcggg tcccgccgtg
    18241 gccttcgtcc gcgccactgg ggcggccctc gacggccgac agcgcgacag cgtcggagcg
    18301 acattgtcgg agctggccac gcttctgcgg gagatgggcg tcgacgacgc agtcaacctc
    18361 gacggcgggg ggtcgtcgac gctggtgtac cgggaaccgg ggcccgaccc cggcaggatc
    18421 gtgaacgatc cgtcggcgtc accgcgcctg gtgcccaacg gtggcggtgt ctacacgacc
    18481 tgacccgccc cggtgccccg gagtgcgacg gatcgatcca acgggcagcg tggtggtccc
    18541 gtaccgacgt cctcgaggag agccccgtga cgagtgccga gctgctgaca gat
//
LOCUS       DASRSC010000047         7947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1816760,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000047 DASRSC010000000
VERSION     DASRSC010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1816760"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /gene="pgi"
                     /locus_tag="VFE39_02335"
     CDS             <1..1011
                     /gene="pgi"
                     /locus_tag="VFE39_02335"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010314633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET6257466.1"
                     /translation="LTNAHVARKWLLDGLGSDDEAVAKHFVAVSTNAKEVSAFGIDED
                     NMFGFWDWVGGRYSLDSAIGLSLMVAIGKEQFGEFLAGMHAIDEHFRTAPLEENLPAI
                     AGLLNVWYVNFFGTQTHAVLPYSQYLHRLPAYLQQLTMESNGKSVRGDGTPVTTATGE
                     IFWGEPGTNGQHAFYQLLHQGTRLVPADFIGFAEPIHEVPVDDHTDMHDLFMSNLFAQ
                     TSALAFGKTAEEIAAEGTPADIVPHKVMPGNRPSSTILARKLTPSVLGQLIAFYEHMT
                     FVEGTIWGIDSFDQWGVELGKVLAKQLGPVLTAAEPDTSGLDSSTAALVRRYREWRGR
                     PG"
     gene            1161..2063
                     /locus_tag="VFE39_02340"
     CDS             1161..2063
                     /locus_tag="VFE39_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET6257467.1"
                     /translation="MADVRDEGRDAVPVDGASAGAASGEVQRAGDTASVGSTPPGPAH
                     DGPAPAGAPGVAPAGLRQPLQHPPPRPGLGALVAVLLALAAAVGLYTAFTGVGPALVD
                     AALLSESLEVRSSALTTLAVLVTDIGSTVSMAVLATAVGVWCWYVGRRADALLAIGAM
                     AGAAGVFRVLKALFDRPRPPAEDQLVSAANESLPSGHATMSIVVIGTLVVLAWSGRSA
                     AARAALVAVATLWVGAIGATRIYLGVHWFSDVLAGWLVGAAWLALCVVLWSSWRAHHQ
                     PTTRLTGTDPVGSAGRDPGASDAA"
     gene            complement(2154..2960)
                     /locus_tag="VFE39_02345"
     CDS             complement(2154..2960)
                     /locus_tag="VFE39_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET6257468.1"
                     /translation="MPELPEVERARRALEGALDREIRSVDDNDEWVCRPHRPGDIARA
                     LVGGRLVAAHRRGKTMWCDTVRRDGEAGPRLGVHLGMGGRIIVTDTDGERAGGEIERP
                     DRTPPKPEWDRFTMVFADGGQLRLFDKRRLGRVRLEPDVDALGPDAEVISREDFRTRV
                     GRGQAPVKARLLDQSVLAGVGNLLADETLWQAALSPSAPAGALAGATLDRLHDALERA
                     IKAAIANGGVHTGEVIPFRHADAHCPRCGAPMAHGTVGGRSTWWCSAEQG"
     gene            3562..3771
                     /locus_tag="VFE39_02350"
     CDS             3562..3771
                     /locus_tag="VFE39_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6257469.1"
                     /translation="MPDEQEDPLIPTGEVARRLGVTPHAVGRWVARGMITPAVRTPGG
                     RYRFRWSEVSAQLQELQDRDSTENR"
     gene            3988..4182
                     /locus_tag="VFE39_02355"
     CDS             3988..4182
                     /locus_tag="VFE39_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6257470.1"
                     /translation="MLTGEYDYLTTPEDGQRTAAQIKNASFIEMKEIGHFPMSENYGV
                     FRGYLRQTLETLRKKQTAQV"
     gene            complement(4248..5183)
                     /locus_tag="VFE39_02360"
     CDS             complement(4248..5183)
                     /locus_tag="VFE39_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET6257471.1"
                     /translation="MTDHHLEPAPETTIDVFDRTTTPVLTVDPGDTVVVRSLDAIGHL
                     ERQQKPGEERPLMFNPRKGHCLTGPIEVRGAEPGSVLAVRLVSLRPGDWGWTVAAARD
                     NWLTRRLGLADGPPSWLLWELDVERGIGINDRGHSVALAPFLGVIGMPPDAAGEHSTT
                     PPRAAGGGNIDCRELVAGSTLYLPVTVPGALLHLGDGHAAQGDGEVGGTAIECPMTTE
                     VVLDLITDPAIPGIHAVTPAGRITFGFHQDLNEAMAEALDAMLVWMQALLALDGDDAK
                     ATALAVASTAVDLRVTQVANEAWGVHAVLPHGAIG"
     gene            complement(5289..5930)
                     /locus_tag="VFE39_02365"
     CDS             complement(5289..5930)
                     /locus_tag="VFE39_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6257472.1"
                     /translation="MRVVLAEDSLLLREGLVRLLDEAGASVVAAVGDGTALVEAVHEH
                     RPDVAVVDVRMPPTFTDEGLRAALEVRRTLPGTAILVLSQYVEESYASDLLTAGGGVG
                     YLLKDRVAKLADLSDALERVVDGGTVLDPEVVSALFAQRRRRDPLATLSPREREVLGL
                     MAEGRTNTAIGRSLVITQGGVEKHISSIFTKLDLPPSGDDHRRVMAVLTWLGV"
     gene            complement(5931..7256)
                     /locus_tag="VFE39_02370"
     CDS             complement(5931..7256)
                     /locus_tag="VFE39_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HET6257473.1"
                     /translation="MTGLASHPAASPVAGAVGVGRFWRGMGYLLGGLPLAIVAFVIAI
                     AGFSAGVGTLVVWVGLPVLVLTLRAVRGLAVVERRSTEWATGRPLPPHHYREARGRGI
                     PRWFNALGEPQSWRDLLHAVVAFPVRLVTFVLAVAWTVGGLGGLLYVTWQWALPDNEE
                     GGGLFWLMTGNGGRLGEIAVNMVLGAVMVATSPAVVRGLVATRAGLARGLLTNQTAAL
                     RARATQLESSRRAAVQAEAQTLRRLERDIHDGPQQRLVRLNMDLETVARRLDDDPDKA
                     RPLVVEALEQSREALSELRALSRGIAPPILADRGLGPALAAAAARCPVQVSLDVALPQ
                     GRRLAAAVENTAYFVVTEALTNVAKHAHATLCTVLVSADGERVLVQVRDDGRGGAHLG
                     KGHGLAGLADRLATVEGGLDVVSPLGGPTVLTAEIPLVGLGESGPGEDH"
     gene            7361..>7947
                     /locus_tag="VFE39_02375"
     CDS             7361..>7947
                     /locus_tag="VFE39_02375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET6257474.1"
                     /translation="MAEDVWRTVDDYFGRMLLGPDPVLDAALEANAIGGLPAIDVFPA
                     QGKLLHLLARSIGARRILEIGTLGGYSTIWLARALPPDGQLLTCEIDPRHAQVATSNL
                     AMAGFAEIVDVRVGPALDTLSTLGPDVDGRFDLTFIDADKAANTEYFEHALRLSRPGS
                     MIIVDNVVRNGRVVDAGSDDPSVLGTRRLAEVVAQ"
BASE COUNT         1163 a         2922 c         2747 g         1115 t
ORIGIN      
        1 ctgaccaacg cgcacgtcgc gcgcaagtgg ctgctggacg ggctgggcag cgacgacgag
       61 gccgtggcca agcacttcgt ggcggtctcg acgaacgcga aggaggtgag cgctttcggc
      121 atcgacgagg acaacatgtt cggcttctgg gactgggtgg gcgggcgcta ctccctcgac
      181 tcggcgatcg gcctctcgct catggtcgcc atcggcaagg agcagttcgg ggagttcctt
      241 gccgggatgc acgcgatcga cgagcacttc cgcaccgcac cgctcgagga gaacctgccg
      301 gccatcgccg ggctgctcaa tgtgtggtac gtgaacttct tcggcactca gacccacgcc
      361 gttctcccct acagccagta cctgcaccgg ctgcccgcct atctccagca gctcacgatg
      421 gagagcaacg gcaagtcggt gcgtggtgac ggcacccccg tcaccaccgc gaccggcgag
      481 atcttttggg gcgagccggg cacgaacggc cagcacgcct tctaccagct gctgcaccag
      541 ggcacgcggc tcgtccccgc cgacttcatc gggttcgccg agccgatcca cgaggtgccg
      601 gtcgacgacc acacggatat gcacgacctg ttcatgtcga acctgttcgc tcagacgtcg
      661 gcgctggcgt tcggcaagac ggccgaggag atcgcggccg agggcacccc ggcggacatc
      721 gtgccgcaca aggtgatgcc ggggaaccgg ccgtcgtcca cgatcctggc ccggaagctg
      781 acgccgtcgg tgctcggtca gctcatcgcg ttctacgagc acatgacctt cgtcgagggc
      841 acgatctggg gtatcgactc gttcgatcag tggggtgtgg agctcggcaa ggtgctggcg
      901 aagcagctcg gcccggtgct caccgcggcg gagccggaca ccagcgggct cgacagttcc
      961 acggcggcgc tggtgcgccg ctaccgggag tggcgcggcc gcccgggctg agatccgggg
     1021 caactcgtgg gcgcgaaact ggcgactcgc gggtcggatt cgggcgactc gcgggcggga
     1081 aacgggcgac tcgcggggtg gggtggacct gatcaggtgc tgcccgggcg ggtggagccg
     1141 gggcggcgca ggatggagcg atggcggacg tgcgggacga aggccgcgac gcggtcccgg
     1201 tcgacggcgc gtcggcgggc gccgcgtccg gtgaggtgca gcgagccggc gacaccgcgt
     1261 cggtgggcag cacgcccccc ggtcctgcac acgacgggcc ggcacccgcc ggggcccccg
     1321 gagtggcgcc cgctggtctc cggcagcccc tgcagcaccc gcccccgcga ccaggactcg
     1381 gcgcgctggt cgctgtgctg ctggcgctcg ccgctgcggt cgggctgtac accgcgttca
     1441 ccggtgtcgg cccggcgctg gtggacgcgg cgctgctcag cgagtcgctc gaggtccgct
     1501 ccagtgcgct caccacgctc gccgtgctcg tcacggacat cgggagcacg gtgtcgatgg
     1561 ctgtgctcgc cacggctgtg ggcgtctggt gctggtatgt cgggcggcgc gccgacgcac
     1621 tgctcgcgat cggcgcgatg gccggagccg ccggcgtgtt ccgcgtgctc aaggcactgt
     1681 tcgaccgccc acgcccgccc gccgaggacc agctggtcag cgccgcgaac gagtcactgc
     1741 cgtcggggca cgcgacaatg tcgatcgtgg tgatcggcac gctcgtggtg ctcgcctgga
     1801 gtggccggag cgcggcggcg cgggcggcgc tggtggcggt tgccaccctg tgggtgggtg
     1861 ccatcggggc gacccggatc tacctcggcg tgcactggtt cagcgacgtg ctcgcaggct
     1921 ggctcgtcgg cgcggcctgg ctagcgctgt gcgtcgttct gtggagctcg tggcgcgccc
     1981 accaccagcc aaccacgcgg ctcaccggga cggatccggt aggaagtgct gggcgtgacc
     2041 caggcgcctc ggacgcggcg tagtccaggt actggaacga cccgatcccg cggataccga
     2101 acgcggcgat cgctgttcgc tccgatgccc cacccgggcc ggaggttccc cggtcatccc
     2161 tgctccgccg agcaccacca cgtcgagcgg ccgcccacgg tgccgtgtgc catcggcgct
     2221 ccgcaccggg ggcagtgtgc gtcggcgtgc cggaagggga tcacctcgcc cgtgtgcacc
     2281 ccgccgttcg cgatcgccgc cttgatcgca cgctccagcg cgtcgtgcag tcggtccagg
     2341 gtggcgccgg ccagggcgcc cgcgggcgcc gacggagaga gcgcggcctg ccacagtgtc
     2401 tcgtcggcga gcagattgcc caccccggcc agcaccgact ggtcgagcag ccgcgccttg
     2461 accggtgcct gcccgcgccc gacgcgcgta cggaaatcct cgcggctgat cacctccgcg
     2521 tccgggccca gagcatccac gtccggttcg aggcggacgc gcccgagccg ccgcttgtcg
     2581 aacaggcgca gctgcccccc gtcggcgaag accatcgtga accggtccca ctcgggcttg
     2641 ggcggggtgc ggtcgggccg ctcgatctcg ccgccggccc gctcaccatc cgtatccgtc
     2701 acgatgatcc gcccacccat tccgagatga acgccgagcc gcggaccggc ctcgccgtcg
     2761 cggcgcaccg tgtcgcacca catcgtcttg ccgcgccggt gggccgccac gagtcgtccc
     2821 ccgacgaggg cgcgcgcgat gtcgcctggt cggtgtgggc ggcacaccca ctcgtcgttg
     2881 tcgtcgacgg accggatctc ccggtccagc gcaccttcga gggctcggcg cgcgcgctcg
     2941 acctccggca actccggcac acacccagtc tgacggcgac gcggaatcgg cgcccgccga
     3001 cgtcgcgttc agcgccgcag ccgagcccgt tgtcgtgcga ccgtcatggc ggaaccggca
     3061 cagccactcc cctgcgctgc ggctgctcca cggtcggagc ctcattaaca cgcccgagcc
     3121 gttcctgtcg atcgtgctgc tgacgatttc agcgccgttg tacgcgcgcc ggatcacctc
     3181 agcccgctca gccagccagc gcggtgcgca cggcgcctcc acgaccgtcc gaccgcccgg
     3241 agttgtgcag gccggacaga tccccgtcgg cggatgctgc gcgagcagtc ggtggatgag
     3301 cgccggccac tcggcaagcg caccggccag acgttcgtag ggcgacgtgg gcagatcggt
     3361 acggttcacg acgggtccgc tccagctagt ggatcaagga cccggctagg tgttaccgca
     3421 cctggtcggg gccgcctccg tttcggggaa ggcgcacccg caccgtaacg ctactgatcg
     3481 gactgatgct accaatgaca catttcgtac ctatgctacc gacggccccg atgtgaccgg
     3541 cgttcgctct agcgtcaagg ggtgcccgac gagcaagagg acccgctgat cccgacgggt
     3601 gaggtggccc gccgtctcgg cgtgacgcca cacgcggtcg gccgttgggt ggcgcgcggg
     3661 atgatcactc cggccgttcg cacgcccggt ggtcgctacc gtttccgctg gtcggaggtg
     3721 tcggctcagc ttcaggagct acaggaccgg gacagcaccg agaaccggta gcgcgagcac
     3781 tccacaccgc tcttccatgt ggtcgacgga ccagatctcc cggtgcagcc accttcgagg
     3841 gctccgcgcg cgccccgcac gccctcgccc gccgcgcggg tgggctcggt cgcccgtcgg
     3901 catcgtcgag caggagccga gccgaccaat tcactgagct actggtcccg ctcgttgggc
     3961 gggggttcag atgggtatcc gagctcgatg ctgaccggcg agtacgacta cctcaccaca
     4021 cccgaggacg ggcagcgcac cgcggcgcag atcaagaacg cctccttcat cgagatgaag
     4081 gagatcgggc acttcccgat gagcgagaac tacggggtct tccgcgggta cctccggcag
     4141 actctggaga cgctgcgcaa gaagcagacc gcgcaggtct gaggggatcc gccgggtcgt
     4201 gtcccgccgt gccaggagac gcggagggac acgacgaacc ggccaggtca gccgatcgcc
     4261 ccgtgtggca gcacggcgtg cacgccccag gcttcgttgg cgacctgcgt gacccgcagg
     4321 tcgaccgccg tgctggccac agccagcgcg gtcgccttgg cgtcgtcgcc gtcgagtgcg
     4381 agcagtgcct gcatccacac cagcatcgcg tcgagtgcct cggccatggc ctcgttgagg
     4441 tcctggtgga agccgaaggt gatccgcccg gcgggcgtga cggcgtgaat gcccggtatg
     4501 gccggatcgg tgatcaggtc gaggacgacc tcggtggtca tcgggcactc gatcgccgtg
     4561 ccaccgacct ccccgtcgcc ctgcgccgca tggccgtcgc cgaggtgcaa cagcgctccg
     4621 gggacagtga ccggcaggta cagcgtggaa ccggcgacga gctcccgaca gtcgatgttc
     4681 cccccacccg ccgcccgcgg cggggtcgtg gagtgctcgc ccgccgcgtc cgggggcatc
     4741 ccgatcaccc cgaggaacgg ggcgagcgcc acgctgtggc cgcggtcgtt gatgccgata
     4801 ccccgctcga cgtcgagctc ccagagcagc caggacggcg ggccgtccgc gagccccagc
     4861 ctgcgcgtca gccagttgtc cctcgctgcg gccaccgtcc agccccagtc gccgggccgc
     4921 aacgacacca ggcgcaccgc gagcacgctg cccggctcgg ccccgcgcac ctcgatcgga
     4981 ccggtgagac agtgcccctt ccgggggttg aacatgagcg gccgctcctc gcccggcttc
     5041 tgctggcgct ccaggtgacc tatggcgtcc agcgaccgca cgaccaccgt gtcgcccggg
     5101 tcgacggtga ggaccggcgt cgtggtgcgg tcgaagacgt cgatcgtcgt ctccggggca
     5161 ggctcgaggt ggtggtcggt catgcagctc ctccatgggt accgggcctc cgttgggccc
     5221 ggccgaacca gcggacggtg tccgccgttc gagacgatcg tggcaggtga tcacgacccg
     5281 ccgtcgcctc agacccccag ccaggtgagg actgccatca cgcggcggtg atcgtcaccc
     5341 gacgggggca gatcgagctt ggtgaagatc gacgagatgt gcttctcgac gccaccctgg
     5401 gtgatcacca gcgagcgtcc gatcgccgtg ttcgtccggc cttcggccat gagtcccagc
     5461 acctcgcgct cgcgaggcga caacgtggcg agcgggtcgc gacggcgccg ctgcgcgaac
     5521 agcgccgaga caacctccgg gtcgagcacc gtgcccccgt cgaccacgcg ctccagcgcg
     5581 tcggacaggt cggcgagctt ggcgacccgg tccttgagca ggtagccgac gccgcccccc
     5641 gccgtgagca ggtccgaggc atacgactcc tcgacgtact gcgacagcac gaggatggcg
     5701 gtccccggta acgtccggcg tacctcgagt gcggcgcgca gaccctcgtc ggtgaacgtg
     5761 ggaggcatcc ggacgtcgac gacggcgacg tcgggccggt gctcatgcac ggcctcgacc
     5821 agcgccgtgc cgtcgccgac ggcggccacc accgacgcgc ccgcctcgtc gaggagccgg
     5881 accagcccct ccctcagcag gagcgagtcc tccgccagca ccacacgcac ctagtgatcc
     5941 tcccccggtc ccgactcccc cagcccgacg agcgggatct cggcggtcag cacagtaggc
     6001 ccgcccagcg ggctcaccac gtccagccca ccctcgaccg tcgccagccg gtcggccaac
     6061 ccggccagac cgtgcccctt gccgagatgg gcaccgcccc gcccgtcgtc ccgcacctgg
     6121 acgagcaccc gttcaccgtc ggcgctcacc aacaccgtgc acagcgtggc gtgcgcgtgc
     6181 ttggccacgt tcgtgagggc ctcggtcacc acgaagtacg ccgtgttctc caccgccgcg
     6241 gcgagccgcc gaccctgcgg gagcgcgacg tcgagggaca cctgcaccgg gcaccgggcg
     6301 gcggccgccg cgagcgcagg cccgagcccc cggtcggcga ggatcggcgg ggcgatgccc
     6361 cgggagagcg cacgaagctc actcagcgcc tcccggctct gctccaacgc ctccacaacg
     6421 agaggccgcg ccttgtccgg gtcgtcgtcc agacgacgag ccacggtctc cagatccatg
     6481 ttcaaccgca ccaggcgctg ctgcggaccg tcgtggatat cacgctccag gcgccgcaac
     6541 gtctgcgcct ccgcctgcac cgcagcccgc ctgctcgact ccaactgagt agcgcgggca
     6601 cgcagcgcgg ccgtctggtt ggtcagcaaa ccccgcgcaa ggcccgcgcg ggtcgcaacg
     6661 agcccgcgca ccacggcggg cgacgtcgcc accatcaccg cgcccagcac catgttgacc
     6721 gcgatctccc cgagccggcc gccgttgccg gtcatgagcc agaagaggcc gccgccctcc
     6781 tcgttgtcgg gcagtgccca ctgccaggtg acgtacagga ggccgccgag cccacccacc
     6841 gtccatgcga cggcgaggac gaacgtcacc agccggaccg ggaaggcgac taccgcgtgc
     6901 agcaggtcgc gccacgactg cggctcaccc agcgcgttga accaccgcgg gatgcctcgc
     6961 cccctggcct cgcggtagtg gtgcgggggc agtggcctgc cggtcgccca ctcggtggac
     7021 cggcgctcga cgaccgcgag cccccgaacg gcccgcagcg tcagaaccag cacgggcagc
     7081 ccgacccaga ccacgagcgt ccccacgcct gccgagaacc cggcgatggc gatgacgaac
     7141 gcgacgatgg ccagcgggag gccaccgagg aggtagccca ttccccgcca gaacctgccg
     7201 actcccacgg cacctgcgac aggcgatgcg gcggggtgcg atgcaagtcc ggtcatgccg
     7261 ctaccctcgc cccccgtcgt agccgccccc agccggtgcg cctccgcagg gagggtaggg
     7321 ccaacccgaa cccgtgcgcg ccccattagg gttccctctc atggcggagg acgtgtggcg
     7381 gacggtcgac gactacttcg gccggatgct cctcggtccc gatccagtgc tcgacgccgc
     7441 gctggaggcc aacgccatcg gcggtctgcc ggcgatcgac gtcttcccgg cgcaggggaa
     7501 gctgctgcac ctgctggccc gctcgatcgg cgcccggcgg atcctggaga tcggcacgct
     7561 gggcggctac tccacgatct ggctggcccg cgccctcccg ccggacgggc agctgctcac
     7621 gtgtgagatc gacccgcgcc acgcccaggt ggccacgtcc aacctggcca tggcgggttt
     7681 cgccgagatc gtcgacgtgc gcgtcgggcc ggcgctcgac acgctcagca cgctcggccc
     7741 cgacgtcgac ggccgcttcg acctcacgtt catcgatgcg gacaaggccg ccaacacgga
     7801 gtacttcgag catgcgctgc ggctctcgcg gcccggcagc atgatcatcg tggacaacgt
     7861 ggtgcgcaac ggccgggtgg tcgacgcggg cagtgacgac ccctccgtgc tgggcacccg
     7921 aaggctggcc gaggtcgtag cgcagga
//
LOCUS       DASRSC010000048        40426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1489081,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000048 DASRSC010000000
VERSION     DASRSC010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 40426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40426
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1489081"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..413
                     /gene="mnhG"
                     /locus_tag="VFE39_02380"
     CDS             75..413
                     /gene="mnhG"
                     /locus_tag="VFE39_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H(+) antiporter subunit G"
                     /protein_id="HET6257475.1"
                     /translation="MLDTVSAAFLLLGALSSLLGALGMVRLPDLLARLQAATKPQTLG
                     LLLILAGTALRVELESAATLVLVALFQVITAPVISQLAGRSAYRSGTVRRDLLVVDEL
                     ARELPPDRPS"
     gene            535..1056
                     /locus_tag="VFE39_02385"
     CDS             535..1056
                     /locus_tag="VFE39_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET6257476.1"
                     /translation="MPLYALGDVEPDIHPDAYVHPDAVVIGNVTIGAEASVWPSAVLR
                     GDDGRIEIGPRTSIQDGSIIHCTPAEPTIIGAEVTVGHNVHIEGAIIGDRALISSGSV
                     VLNGATIGAGAVVGAGAVVSPNAEVPPRRMALGVPARVREGHEVPEGYFDHAVQSYIQ
                     RGKRFRAELRRLG"
     gene            1053..1733
                     /locus_tag="VFE39_02390"
     CDS             1053..1733
                     /locus_tag="VFE39_02390"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET6257477.1"
                     /translation="MMARPLEEIVEAGWARALAPVAPVIADMGEFLRSELAAGRRYLP
                     AGANVLRAFTQPFDDVRVLIVGQDPYPTPGHAIGLSFSVSPQTRPVPRSLANIFREYT
                     EDLDHPTPSTGDLTAWTEQGVLLLNRVLTVEPGNPGSHRSKGWETVTEQAIRALVDRD
                     GEPLVAILWGRDARNLVPLLVDVPCVESAHPSPMSADRGFFGSRPFSRANALLEELGA
                     EPVDWKLS"
     gene            complement(1821..2012)
                     /gene="rpmB"
                     /locus_tag="VFE39_02395"
     CDS             complement(1821..2012)
                     /gene="rpmB"
                     /locus_tag="VFE39_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET6257478.1"
                     /translation="MAAVCDVCGKGPGFGMSVSHSHRRTHRRWNPNIQTVRARIGGGN
                     RQRLNVCTSCLKAGKVARA"
     gene            2131..3804
                     /locus_tag="VFE39_02400"
     CDS             2131..3804
                     /locus_tag="VFE39_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET6257479.1"
                     /translation="MAPTLDSALLTGWTRAATQALERHRAEIDRINVFPVPDGDTGTN
                     LLLTMRAAAEAVRRVLRENGDGPARGVAAVAGALARGALVGARGNSGVILSQVLRGVA
                     DAVAAVAGPTPAGAVLADALRRAHRLATAAFTRPREGTVLSVLGAAAGAAAAAGSDRL
                     DDVALAAAEAAAGAVQATTGQLPELARAGVVDAGGLGLYLVLDALAGLVSGRTCTVRL
                     PATGTPVRGPARSGDLLVAERESGSAIYDYEVMYLLEGAGPDQVDVLRAELQALGDCV
                     SVVGDGGEDRGGLWNVHVHCTDIGGAIEAGLAAGRPHRITVIRFADQVGDLPKPQRDA
                     RFVRERAVLMVVGGSEIAELARSAGAAVLERPRDREVDEADLAAALAGTRARHVVLLP
                     DDSELTAVAERAAAVARRDGQEVLVVPTASVLQGLSALAVHDPERRAGDDVVAMAEAA
                     AGTRTGGLLVAESEALTWVGHCQPGDVLGVSDGEVVLIAPDLAVGGLWLAHRMLTAGG
                     ERVTVLLGEGADEALGDGLAVDLRLTHPEVDVVVHRGGQTDYPIVLGVE"
     gene            3801..5999
                     /gene="recG"
                     /locus_tag="VFE39_02405"
     CDS             3801..5999
                     /gene="recG"
                     /locus_tag="VFE39_02405"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET6257480.1"
                     /translation="MTDVDMLSPLLPLVGKRAADLLSTQLEIHTVGDLVRHYPRRYVD
                     RGKLTDIAGLEIGEHATLVAEVEKATLRDMRARRGKLLNVVIRDEKGASLDCTFFNGH
                     KVQHVIRPGVRAVFSGKVGVFNKKLQLTHPQFEPLDEADEIRPFLSVYPATGKINSQE
                     IARCVRQVLDLLDDPTDPLPAALRQREGLAELGRALRRIHVPETEADFHAARNRLVWD
                     EAIGVQLALALRRQATVSRPAAACPPKPGGLLDAFDAALPFTLTAGQHAVGAEISADL
                     AREHPMNRLVQGDVGAGKTVIALRAMLQVVDGGRQAAMLAPTEVLAAQHARSLRTLLG
                     PLGQAGELGGAEQSIGVTLLTGSLGAKARRQALLDAQSGAAGIVVGTHALIQDHVGFA
                     DLGLVVVDEQHRFGVEQRDALRGRGEVAPHMLVMTATPIPRTVAMTVYGDLEVSALRE
                     LPGGRSPIATTVVPLAEKPGWLQQVWRRVREEAAAGHQVYVVCPRVGGDPAKGDPDAD
                     LVDLDDPDMGADADDASARRPPLAVLEVLPKLAEGALHGLRLGVLHGKLPPDEKDAVM
                     RAFERGELDVMVATTVIEVGVDVPNATGMVILDAERFGLSQLHQLRGRVGRGSAPGVC
                     LLVTEAPEASTARERLDAVAGTTDGFELARLDLELRREGDILGALQSGRRSGLRLLSL
                     LRHEEIIAKARVYAAHIVADDPGLRRHPGLAALVGETVGDEERAAYLDKV"
     gene            complement(6019..6870)
                     /locus_tag="VFE39_02410"
     CDS             complement(6019..6870)
                     /locus_tag="VFE39_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257481.1"
                     /translation="MIPRVQAGQSAAFAEGRRLREAAEMRRRRRHVAGHHGHRRNPQP
                     PWLLLLAVPLVAGGASALAMVMVSLILFGVAIMAGAAPMVMTGAIAFVAVHRHRRYTA
                     LQRRSPAHAPHPVRNEARPEVAWNDARTRFQTLRSAYAAFECDPMEVLRLPALADVSV
                     PSTARFVDAFAEAQALDSDKFPPGKHAAKFVAAVERADRAWHAARDAAERIRLSGLSP
                     AERGTVERIIKLLTTARDSDSDPERLAAYARARSELAKLDRAGVIHLPHSAGAALDAA
                     ARGALPA"
     gene            7068..10451
                     /locus_tag="VFE39_02415"
     CDS             7068..10451
                     /locus_tag="VFE39_02415"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HET6257482.1"
                     /translation="MFRKVLVANRGEIAIRAFRAAYELGVSTVAIFPYEDRNSLHRAK
                     ADESYQIGEEGHPVRAYLSVDEIIAAARRAGADAIYPGYGFLSENPELAEACAQAGIT
                     FVGPPADVLHLTGNKARAVAAAREAGVAVLDSSEPSDDVDTLVAAAEDVGFPIFVKAV
                     AGGGGRGMRRVEEPGDLRESIEAAMREAESAFGDATVFLEQAVVNPRHIEVQILADAD
                     AEGEVVHLYERDCSVQRRHQKVIEIAPAPNLDPELRDTICADAVAFARHIGYVNAGTV
                     EFLLDERGHHVFIEMNPRIQVEHTVTEEVTDRDLVIAQLRIAAGATLAGLGMRQEDIH
                     VHGAALQCRITTEDPSNGFRPDTGVIGAYRSPGGAGVRLDGGTTHTGAEVSAHFDSML
                     VKLTCRGRTFRTAVRRAKRAVAEFRIRGVSTNIPFLAAVLADPDFQEGRITTSFIDER
                     PQLLTARQPADRGTRLLTYLAETTVNRPNGTRPKGVEPVDKLPVCDLGPAAPDGSRQL
                     LRELGPEGFASWLRGRDAVAVTDTTFRDAHQSLLATRVRTRDLLAVAPYVARTMPQLF
                     SLECWGGATYDVALRFLAEDPWERLAALREAVPNICTQMLLRGRNTVGYTPYPTEVTD
                     AFVEEAARTGMDIFRIFDALNDVEQMRPAIEAVRRTGTTVAEVALCYTGDLSDPAEPL
                     YTLDYYLRLAEQIVDAGAHVLAIKDMAGLLRPPAARTLVTALRERFDLPVHLHTHDTA
                     GGQLATLVAAIEAGVDAVDAAVASMAGTTSQPALSSLVAATDHTARATGLDLQACCDF
                     EPYWEAVRKVYAPFESGLASPTGRVYHHEIPGGQLSNLRQQAIALGLGDRFELIEDCY
                     AAADRMLGRLVKVTPSSKVVGDLALHLVGAGVEPKDFEAAPGDFDVPDSVIGFLRGEL
                     GDPPGGWPEPFRTRALAGRSAEKEAVDLSIADRRALRDDRRPALNRLLFEEPAKGYVT
                     HLETYGDTSVLSTKDFLYGLEPELEHTVDLEPGVTLLIELEAISEPDERGYRNVLATL
                     NGQMRPVSVRDHAVASDVKAAEKADRSNPQHVAAPFAGVVTLSVDEGDSVESGQTVAT
                     IEAMKMEASITAHQAGTVARLAIGEVQQVEGGDLLLELS"
     gene            10507..11055
                     /gene="rsmD"
                     /locus_tag="VFE39_02420"
     CDS             10507..11055
                     /gene="rsmD"
                     /locus_tag="VFE39_02420"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET6257483.1"
                     /translation="MRIVAGAAGGRRLLVPPKGTRPTSDRVREALFSALENDPGIEGA
                     AVLDLCAGSGALGLEALSRGADHALFVESDRRAATVLRRNVAAIGLAGAVVRSAPAAT
                     VLGAAADRAYDIVLVDPPYGVPDAEVAGWLTAAAAHGWLADAATVVVERASRGGSFPW
                     PEPLRGVRERRYGETALHVALR"
     gene            11080..11562
                     /gene="coaD"
                     /locus_tag="VFE39_02425"
     CDS             11080..11562
                     /gene="coaD"
                     /locus_tag="VFE39_02425"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET6257484.1"
                     /translation="MRRAVCPGSFDPPTNGHLDVISRASALFDEVVVAVLVNKSKKSM
                     FSVDERMAMLAEIAVPYPNVRVGSFHGLLVDYCRDNDIRAIVKGLRAVTDFDYELQMA
                     QMNQRLSGVDTLFMSTSPDYSFVSSSLIKEVATYGGDVAHLLPESVHRRLLERIAERS
                     "
     gene            11641..12387
                     /locus_tag="VFE39_02430"
     CDS             11641..12387
                     /locus_tag="VFE39_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HET6257485.1"
                     /translation="MYRVFESLDALVTVVEEARGVPMTSNCVVPRGDVLELLDDVREA
                     LPGELDDAQDVLDRRDELVDEAAQEAEQTRSGAQADAAEMLAEARAEAERMVGEARAE
                     AEQTLAQARHDAERAVTEGRRQYTELTDRAQAEADRLAHAGRAAHDRYIADGQAEQAR
                     LVSQTEVVQAAHAEAARLVDGAEREVDRLRRECDNYVDAKLAEFEDALGKALRTISRG
                     RTQMWRGGSPNSAPAGIPNGRSGTGMDLID"
     gene            12628..13560
                     /locus_tag="VFE39_02435"
     CDS             12628..13560
                     /locus_tag="VFE39_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HET6257486.1"
                     /translation="MLGDGTPTTASELSSEAILRARPDEPASGWRRAVLRATGGLVNP
                     GPSPAEVQWRDQLHRIRHPLLQSHRIAVTSIKGGVGKTTVVTLLGLVMAENRGDRVIV
                     LDANPDAGTLADRLTGESSVTVRELLRDLHRIHSWTEVSRYTSLAGRLQVLASEQDPA
                     SSDAFSREEYEHICGLLDRFFNIIITDSGTGLVHSAMEGTLKLADSLIVVGAPTVDGA
                     GRASKTLDWLHAHEHTALASEAVVVLSCDRVSAEVDRERIREHFTARCRAVIELPHDP
                     HLATGGRVEIARLRPATRDAAFQLAALMADRFVL"
     gene            13803..14972
                     /locus_tag="VFE39_02440"
     CDS             13803..14972
                     /locus_tag="VFE39_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6257487.1"
                     /translation="MTQPREATGGTRADAAPDEPKAAPAEGPVAATTSAGSPDHSGGS
                     STDSDEFAARPGAEPPTTPRSRPEAASAAELTEDAIVRFRSDRPQRGWRGALYKITGG
                     VVNPGVGAAERARQDQLRRIRRPLPGSHQIAVSSLKGGVGKTTVSACLGLVLAENRGD
                     RVIALDANPDAGTLADRLTTETSVTVRNLLDNIHATHSLTDIARYTSLAGRLQVLASE
                     QDPAMSEAFSRDEYEQVCEVLTRFYNIVITDSGTGLVHSAMEGTLALADSLVVVGAPT
                     VDGASRASKTLDWLIAHGHATQVANAVVVLSCDRMSKEVDLERVRGHFAARCRAVVEI
                     PHDPHLATGGRIELARLRPATHDAFLTLAALLADAFDSSKAPVRQVAPEQVPSLS"
     gene            complement(15127..17304)
                     /locus_tag="VFE39_02445"
     CDS             complement(15127..17304)
                     /locus_tag="VFE39_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257488.1"
                     /translation="MTDEPNTCALPGCSETIEQPDGGGPPRRYCTPAHRAAARKQRHL
                     AAHNNAEPEVPTVAAPAALTVPVTADATPEAVPPPPRSVSPWSAAASWVITPTPPPPP
                     KPEHPRPENRFAARSAGERKAAASLLRKRAVASVAVASLIAGGGGYLLTSTPVPVTPQ
                     AQPPSVPPPTANADQWVAHAEIVLASINKQLDIVALTEASWTRLPEHQRSGQPGALRA
                     LQERKALLEQQRVTLQSQIGAFRNLSDVTTDLAAAQGQLDAVERALRTAPEQTSSLDQ
                     AEAVRNLAAQRDLHLQQRDYNMQELATLRSGVQEALAAPLPDETDQTTPIVERVLALM
                     DHRPDPRPQAEAREQAPAIAPRRESTREERQTISSTAPPDPSRPAGGLVVAQRVDRGQ
                     DGNATESRGRTSGAVTNGAVTNEAVTNGAVTNGAVTNAVRTSGAVIRTAGHVVDTAEK
                     AATNGQSNDDDRSERSEERVRPVSKVVNTVSDLVLGRSDDQRSSSSSNADSSRRSGSV
                     GKSERGGSADPGSQADRRQETQQSAGGDRKVSSTSKPRGKAERAKAAAKAAADRAVAG
                     VLAPAKERGSKAGTTAPGVKSRSGTSTASRNDRGTAAGSTGEGRTSTRATNPGSASHQ
                     NTSSKAKDRTSDGSKAGATTPTGSDSGSRPTGKKTRINSSDSGGDTATGKKTTRTNSS
                     DAGSSKGGSSKASGSKGDSSKGDSSKPGSSKSDSSKPSSSSRG"
     gene            complement(17637..19556)
                     /locus_tag="VFE39_02450"
     CDS             complement(17637..19556)
                     /locus_tag="VFE39_02450"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET6257489.1"
                     /translation="MSTDAAFRVLGPLEVVNGGRPVPVTPGKQRVVLAALLLRANRTV
                     SITDLTDQLWDGAAPFDSRGAVQKYVMRLRQALSGTDVTIQTEPDGYRIELLPGQLDL
                     DRSDALVHQGRRAAEQGDDHGASAGLAEALQLWRAVPPLTNVASPALHRTEVPRLVER
                     YLQALELRIDVDLRLGRHAELSEELIGLVLSHPLRERFWAQWMRALYATGRQGEALEA
                     YREVTRLLADELGIDPGPELRAAHEQILRRTEPPAGSQAIAVSHGVRQLPMAPAGVVG
                     RHREIEQITTLLGAGNSAAARLVVVAGPPGVGKTTVAVHAAHRLVDRFPDGQLFVDLG
                     GYGAAPPRTTEEAVAHFLRALGMPPEGLPRRLDDAIATYRSLTAGRRLLIVLDNVNSP
                     AQVRTLLPGSPTCGVVVTSRHELAGLLVSPGGHRVAVGMLRPDEADTVLRGIVGEKRL
                     AADPAATDELIALCAGLPLALRVAAAHLVTRPHLSLAAYLDELRVGEPVSALRIEGEG
                     QMGVASALEASYVQLDAEQQRLLRLISLVPGDRVALAVVAALTESPIAATATGLDQLA
                     EAGLLDRRADGGYQLHDLTRAFAAQRLTLEDSPAQIDAVRQRLIEAEGRAAHDPARRH
                     DRSGATLREQTVLRS"
     gene            19854..20543
                     /locus_tag="VFE39_02455"
     CDS             19854..20543
                     /locus_tag="VFE39_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6257490.1"
                     /translation="MTTDGLAADRMLLNRTSTAERVAAILRSRISEGFFRPGARLAED
                     TIGTALGVSRNTLREAFRLLSHERLLTHELNRGVFVRTLTVEDVVDLYRVRVLVECGV
                     VRAVDGVPGTDRLVAAVTDGEVAMRRQDWQALGTANLKFHEGLVALADSPRADELMSG
                     VLAELRLVFHVMADPRRFHKPYLARNRGILHLLQQGDGPAAARELELYLRDAEEQLVA
                     AYRHLQPPPFE"
     gene            20749..21804
                     /locus_tag="VFE39_02460"
     CDS             20749..21804
                     /locus_tag="VFE39_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HET6257491.1"
                     /translation="MSSDTGPGRAIPAPPEQGVPPRRPDAEEPTSPAELAGEPRVPGA
                     AELTDDTIVRRRSDRPTAGWRRVVHTVSGGVINPGIGSVERRRQHLTLRIRRELGRPH
                     KIAVTSIKGGVGKTTVAALLGLTLAEHRGDRVVALDANPDAGTLADRLTGEPGATVRD
                     LLDHIDGVDALPDVARFTSLAGRLQVLASEQDPARSEAFHRAEYEQVCAVLTRFYNIV
                     ITDSGTGMVHSAMDGTLAEADTLVVVGALTLDSASRASKTLDWLVAHGHRDLVSRAVV
                     VLSADRTSKELDTARLRSHFSVRCRAVVDVPHDPHLAAGGRIDMGALRPATADAILEL
                     TALVADEFGSSPAARLP"
     gene            complement(21982..22137)
                     /locus_tag="VFE39_02465"
     CDS             complement(21982..22137)
                     /locus_tag="VFE39_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257492.1"
                     /translation="MDDARPLPVMLSRPGPDTDTVHPSVGDTGAGDGGATLGDDVGEG
                     GAEAPPA"
     gene            22178..22339
                     /locus_tag="VFE39_02470"
     CDS             22178..22339
                     /locus_tag="VFE39_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257493.1"
                     /translation="MAITAIRDGGVSFSADGAETAVTKGSMAAVGPYQISVIDAAGDA
                     ARFQVVPPD"
     gene            complement(22462..23208)
                     /locus_tag="VFE39_02475"
     CDS             complement(22462..23208)
                     /locus_tag="VFE39_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257494.1"
                     /translation="MTATEFGPSAPVAAAAIDGTGVLTTAPATRFRRRAMGLAAVAGG
                     ALTAAGFATTVWEVGPEKLDYLDSLVVDPFRSQIAALLLHYGYLGIVPALLALGIMTR
                     RRWTVSGNIGLALSLVGALALPGLLVTDFYDIAIRQSLPPEDAVRVSDAAQALPLSFL
                     IGGPFILAMFVGMLVAAISAWRAGFFHWLGAVPLVVGTVLPMVVPFGTLPNIISGAGL
                     GLFLILVGVAALRMTDEEWVSGRKGAGRVR"
     gene            23410..24063
                     /locus_tag="VFE39_02480"
     CDS             23410..24063
                     /locus_tag="VFE39_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6257495.1"
                     /translation="MSVVLAEDNALLRHGLVRLVEADADLTLAGSAGDLPTLLDLVEK
                     HDPDVVVTDIRMPPTNTDEGIALAAQLRTSRPQTGVLLLSQYAEAAYALRLLANGTAR
                     RGYLLKERVADGDELTDAIRRVAEGGSVIDPIVIEGLVAANRAAPSELDALTPRELEV
                     LGEMAQGKSNASIAAALVLSERAIEKHTNSIFSKLGLSEERDLNRRVSAVLVYLQNR"
     gene            24333..24752
                     /locus_tag="VFE39_02485"
     CDS             24333..24752
                     /locus_tag="VFE39_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6257496.1"
                     /translation="MSTQRVVRVLVVDDQRPFRFAAAAVLARMPGFELVGKADTGEDA
                     VVAAASLRPDLVLMDVRLPGMSGIEAAGLVATEVPGVVVILCSTYARDDLPFSLDAPG
                     VAGYLHKEELRAPVLRELWERCRSSTPAASGPTDAAR"
     gene            complement(24773..27130)
                     /locus_tag="VFE39_02490"
     CDS             complement(24773..27130)
                     /locus_tag="VFE39_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6257497.1"
                     /translation="MGGRLGAVRAVARAELRTGWRGLLALGLLFGLVGGMVLGAVALA
                     ERTATAYPRLVQAVGLDDARVLVPTDQPELAAAVPTLPGVAQARLVHTWVARIEGPAL
                     RFVSVGAGPADPPDLVRPVVVEGREPAPDSLDELLLSEPIAAMTGLGVGSEVTVKMLT
                     LREISMFDVGFGEPDGPEVRMRVVGVGRMPEWSGALCNMLTSPAFAQRYAADAGAIGA
                     FVRLRDEPGAAQSFTDAFVAASTAADARSPSIAAQYLKPFVDLPSATIDPAVTAAENV
                     LVIGLVVFAAVLALGGLQVVGQGLTRLHGSRRNTQEVEFALGMAAVERVGSRVLAALG
                     GAVAGGVVGGGLVIAAGWLEPLGSQARFEPAMGFRPQWDLAVLGGSVLVAVFLLLVAI
                     AVALAGSRKRRRPQPARPPAAVTWGRRWPALLVGVRLALHGRRGTQGVPAAAAVVAAS
                     LAVAGVVAALAFGASLQRLVDTPARYGQAADLQLTDAREPDLARLVADPRVSALDVSA
                     SGHVQISGGPMVEATAREPRKGAIPVATLQGHEPLLRGEIALGPRLADRLQLIPGDSI
                     EFDRPAGGTVRLVVTGIMVTRSEERGGLGDGVVVTRDQLSELVQGPPLIYAGIQAVPG
                     QAGLLFAELSRDLEIFERETPDGVRNLADLLMLPELLALVLAAVAGSGMIHSLVTAAR
                     RHARDVAVLSVLGATPGQVRATLAVMAGATVVPALVVGVPLGLGAARVLWWQVATATG
                     VAGDVAVPPRLLWIVPAVLFGALLLSVVPAGRAARTPPAVALQGR"
     gene            complement(27132..28871)
                     /locus_tag="VFE39_02495"
     CDS             complement(27132..28871)
                     /locus_tag="VFE39_02495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6257498.1"
                     /translation="MSAAPRRRARRSLILSGLMVTVGVTALGVCVGLLGGGPSWPVTA
                     ALALGPLLLGIGAGFSAPARRASTLVLVGASAWAGLAVAVCAGLITMLLVFGRLPIGA
                     QRVLVNPALIAMLVVATLAGPLGRRAGHSARSALNGGQRSPDELLADFAERAGRGTPV
                     DDLLRRLAESMRRDWRLSSVQIWTGEADGPGLARTLVVPQPLHSPAPEPAPLQGDELS
                     TLERAGVAGPGWLRLWLPRLLPGPLGTGHDTRQLRLAPAVHGGRVLALVIIERALDAD
                     TFTAADERALAEVAHRLAIVLRNRALDEALQATLADLRRSNAELQASRTRLVNAADAE
                     RRRIERDLHDGAQQHLVALAVGLRLVRDGLQDVAPGGDTDDAAQGGDGSAAEANLELL
                     DELDHGVRESIQALRDLAHGIYPPLLRDAGLGEALRAAAMRSPLAVSVQVGGLRRHPE
                     QTEAAVYFCCLEALQNSAKHAAGSTVTVTLEETAEELRFAVADTGPGYDVGGMAAGAG
                     LQNMADRLGAIGGSIELRSAAGEGTRVTGRVPLGAATLRPSAAVTGLVTIPVDPRRAG
                     GADSASNGSTMAI"
     gene            complement(28868..29626)
                     /locus_tag="VFE39_02500"
     CDS             complement(28868..29626)
                     /locus_tag="VFE39_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6257499.1"
                     /translation="MVIGKATTAPAGGTVPDSRPDAPDLVVRGLRKTYEAETAPVRAL
                     RGVDLTVHPGEFVGLMGPSGCGKSTLLHLLAGLDRPDEGTISLAGIRTEELSEEKLAR
                     LRRRHVGLVFQFFHLIEHMSALENVALAALVAGARPAEATAGARDLLDTLGLLDKSGE
                     VPAALSGGQRQRVAIARALANRPTLLLADEPTGALDSDGAAEVLDLFRRLHLAGQTIL
                     MVTHSDDVAAGAQRIVRMRDGRILDPAPAHATGP"
     gene            complement(29707..30384)
                     /locus_tag="VFE39_02505"
     CDS             complement(29707..30384)
                     /locus_tag="VFE39_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257500.1"
                     /translation="MPREAHPGSAGQHRRHRVSRRPDGRSTAPAARAPARRIVVPGPA
                     PGPPHRLTAARTPKLGRRALRREQLATLEAPHRRYSLFARLLFVTMDLLYGRAHTLEK
                     FRVLEIVARIPYQIWENAAYRTLTRRHRAAADTGLAHRIHRRIVETREQQDNEQWHLL
                     IVGELVAAQGRRLSQFRFGFLPRMLAAGYYRPTYYARSATTNGCTKWSRRRSWSPGRW
                     PDHRRWC"
     gene            30558..31130
                     /locus_tag="VFE39_02510"
     CDS             30558..31130
                     /locus_tag="VFE39_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019815811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET6257501.1"
                     /translation="MSKHHPTPGRPDPSSPWVFITRELGRRPGNMRTYTRTIPAPAEP
                     ALGLETIAVPAGEPVELDVRLESVTEGVYVSGSVHASLAGECARCLDAVTDAVDVEVS
                     ELFAYPDSVTEETTDEDEVPRVVDEMVDVEQTVRDAVVLALPLAPLCRPDCPGLCPEC
                     GEKWADLAPDHGHETLDPRWAALRERLSAD"
     gene            31160..31342
                     /gene="rpmF"
                     /locus_tag="VFE39_02515"
     CDS             31160..31342
                     /gene="rpmF"
                     /locus_tag="VFE39_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET6257502.1"
                     /translation="MAVPKRRMSRSNTRSRRSQWKATAPTLVACSNRACRATKPPHVA
                     CPTCGTYNGRQVVSPA"
     gene            31443..32129
                     /gene="rnc"
                     /locus_tag="VFE39_02520"
     CDS             31443..32129
                     /gene="rnc"
                     /locus_tag="VFE39_02520"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET6257503.1"
                     /translation="MAHDLLTLALTHRSYAYENGGLPTNERLEFLGDSVLSIVVTERL
                     YRDHPDLPEGQLAKLRASVVNMHALAGVAATLGDGGLGTHLYLGRGEELTGGRTKASI
                     LADATEALIGAVYLEHGLETARGLVHRLFDGLLRGAPLLGAGLDWKTSLQELTAAADL
                     GVPEYRITEDGPDHLKIFTATAVVAGRELGSGEGRTKKEAEQKSAELAWRTLTAEAGA
                     GSQPAPSGPA"
     gene            32139..33119
                     /gene="mutM"
                     /locus_tag="VFE39_02525"
     CDS             32139..33119
                     /gene="mutM"
                     /locus_tag="VFE39_02525"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HET6257504.1"
                     /translation="MPELPEVEVVRRGLAEHVLERKIAAVTVAHPRAVRRHEAGGDDF
                     AARLTGRTITGARRRGKYLWLDLDGGAAGSADAGSACAPPGADAGSACAPPGADAGSA
                     WAPPGDAVLAHLGMSGQMLVADAERPDEKHLRIRFRFVDGGSELRFVDQRTFGGLSVH
                     PLVPAAEGGLLPQPVAHIARDPMDPAFDLDAAVAAIRRRRTGLKRALLDQTVVSGIGN
                     IYADEALWRARLHWARPTETLTRKQGREVLAAASEVMAEALAAGGTSFDALYVNVNGA
                     SGYFDRSLDVYGQADRGCRRCGTPVRRDPFMNRSSFSCPRCQPRPRRAHP"
     gene            complement(33136..34473)
                     /gene="lysA"
                     /locus_tag="VFE39_02530"
     CDS             complement(33136..34473)
                     /gene="lysA"
                     /locus_tag="VFE39_02530"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020632247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET6257505.1"
                     /translation="MTLADLIPTLRTSLSCRLEPGLWPETTRATCDGQLVVGGVDLAH
                     LAARFGTPVLAIDATDVRSRCRAYREALPGVEIAYAAKAFLCRAMARLADQEGLALDV
                     CSGGEVAVAAAGGFPGHRMVLHGNVKSDEDLKTALGVGVGRIVIDSIEEIPTLAALVP
                     ARQQVLLRVVPGVDAGTHAGLTTGTEDQKFGLSIATGAAAEAVRRVLAHPELELVGLH
                     CHIGSQVRSVTRYVEAAERMVAFLAEISREHSIELPQLDLGGGHAVAYAPGEQSVDLA
                     AFARTVPAVVAAACHRFGIAVPRLTVEPGRALVARAGVTVYRVAAIKHGVRRTFVAVD
                     GGMSDNPRPALYGARYPVRRFGRPVSVPSAPVTIVGRHCESSDVLAEDVLLPADVAAG
                     DLLAVPCSGAYHVSLSSTYNQVGRPPVVAVASGAAVPMLRRETIEDLLRRDAG"
     gene            34780..36687
                     /locus_tag="VFE39_02535"
     CDS             34780..36687
                     /locus_tag="VFE39_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6257506.1"
                     /translation="MTDVVSRLRRAPLAQGAAEERHRLTASTGLVALGLDALARCAYG
                     PESIVLALAVAGSAGIGFTVPVTLVIVGLLAVLVVCYRQVINLYPDGGGTYTVARDNL
                     GRRAGLVAAASLVVDYVLNVAVSVAAGVAALTSAFPTLLPYTVELCLAVMLLVAAVNM
                     RGVVAGGRLFALPALVFVGSMAVLIVVGLVRGGPLAPLPASDVVVTPQTVGVLLILAA
                     FANGCAALTGVEAIANATPSFRAPRRVRARRAEAGLGLVLGTLLIGLGVLVQLFDAGP
                     VEGRSLLSLLAEGSVGTGVFYVVVQLSTVVLLGLAANTSYGGLPVLAARLAADGALPH
                     VFGLRGDRQVYRASIVVLSVLAGALLLVSGGQVAVLVPLFAIGVFVGFALCQAGMLRH
                     WWVLRGPRWQSRAALNVLGAVLTGAAALLLAVEKFSAGAWLIVVVIPVLVLVFSGVRT
                     AYTRIGEVLEVDERPQRPRPVASVVVVPVVGISRLAEESLSAALSMGDRVVAVHVVLS
                     GENDDRAAADLLQQRWAEWRPDVTLVLLEAVDEQGRPSRVLGPAIVSYLRTLAEPGTE
                     RVLLLIGEVQPDHWWQRVLFNRRGAVVGRYAERHSQAVVCRLRFRLLPRPKTSPVPQT
                     RGERGLVLPRS"
     gene            complement(36709..37743)
                     /gene="corA"
                     /locus_tag="VFE39_02540"
     CDS             complement(36709..37743)
                     /gene="corA"
                     /locus_tag="VFE39_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET6257507.1"
                     /translation="MSVIDNAIYIDGKRAAVPTSLDHTFEELRQCPDHGRSFCWIGLL
                     RPSQQEIKAVAEEFGLHSLAVEDTINAHQRPKLERYGDVLFVVLRPARYVDPVEVVEI
                     GEVHLFLGPDFVITVRHAEEPDLAEVRKRLESDPGLLDNGPYSVLYAVLDKIVDDYSP
                     VLDGLQVDIDEIEVQVFGGDPGVSKRIYQLSREVIEFQRAVEPLRDIFVELRERLKEH
                     ASESDLELRRALRDVADHATRVMERIEAFRQLLSSILQVNAALVAQRQNEEMARMTEA
                     GYLQNEQVKRISSWAAILFAPTLVASIYGMNFTHMPELTWPLGYPFAVLLMLLLGLGL
                     YAIFKRRGWL"
     gene            37897..38082
                     /locus_tag="VFE39_02545"
     CDS             37897..38082
                     /locus_tag="VFE39_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257508.1"
                     /translation="MDIAGAMLWADLRTAEQTGLHPQLFDPSPAGYLLPARGASAYGY
                     SVAFLVTTGSQFSGSSP"
     gene            complement(38071..38589)
                     /locus_tag="VFE39_02550"
     CDS             complement(38071..38589)
                     /locus_tag="VFE39_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257509.1"
                     /translation="MLAPRPTAGDASPARPVLSGAVGGVLGLPAGAVAAIAAWAFDGN
                     PTTGIVLAAAAAMALAALTTTTGAVVAGALSWACYDGFVLHRFGTLEGGRADVVALGV
                     VVVAAVAAQLVGAVVRHAGWARRPRAVSPPLSRPDVAQGRAVGADRQCSVPAPSGRRR
                     PRAVWARATLRR"
     gene            38769..39089
                     /locus_tag="VFE39_02555"
     CDS             38769..39089
                     /locus_tag="VFE39_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET6257510.1"
                     /translation="MDTSQLLKGVLDLAVLAVLDRADGYGYEVVRGLRAAGLEEVGDA
                     SVYGTLRRLYNAGLLTSYVVPSEEGPHRKYYSVNAPGRERLREMVKTWRAFAETMEGL
                     VGAA"
     gene            39086..40363
                     /locus_tag="VFE39_02560"
     CDS             39086..40363
                     /locus_tag="VFE39_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257511.1"
                     /translation="MNGDNEGTGRSATGAGGTGAPTVRLLKGPAEEYLAGIRSALADL
                     PAPELADILDDVRAHLADLTAELGSEANHAGLVVRLGTPAAYAGELRAAAGYPPAPAP
                     AAARERSRSAAAWFAVAGLVGSVLLVALGAAASAPEAVVLGLLVALAGLPLLTRDDPG
                     VPTVAALPLVRRLTGSRPAPGTTARGVADFLVSLQPAWWVIRAVVAAALVLAVLVSAD
                     PALVVLLSLVTVPTSVWLGHRTRHDRRLLWAVVPLNALAAVLALDALLSTGSGVLGYS
                     APTSGYSAQFHQPGLWQDTDRQILDIRPVDAAGNPLTGVYLFDQDGRAIDTSGSFSCE
                     DDLRSGESAASAARPYPRGTTDYDPRTGDCVTIPPGPLLVAVPSATLQPTSAASTTAA
                     PPATPAVPPSPVPSAVPPAPPAPPVPPVVPPTG"
BASE COUNT         5465 a        14512 c        14762 g         5687 t
ORIGIN      
        1 gcgctggtcg gcttcgtcgg ttcggccacc gcgatgcggc tggtggagcg cagggagcgg
       61 caccggtgaa caccgtgctg gacacggtct ccgcggcgtt cctgctcctc ggcgccctgt
      121 cgagcctgct cggcgcgctc ggcatggtcc ggctgccgga cctgctcgcg cggctgcagg
      181 cggccaccaa accgcagacg ctgggtctgc tgctgatcct cgcgggcacc gcgctgcggg
      241 tggagctgga gagcgccgcg acgctcgtgc tggtcgcgct gttccaggtg atcaccgcac
      301 cggtgatctc ccagctcgcc ggccggtccg cgtaccgcag cggcacggtg cggcgggacc
      361 tcctggtggt cgacgagctg gcccgggagc tgccgccgga ccgcccgtcc tagcggaatg
      421 attgccgcga gacgcacgac gtcgccccgt cgagggcgct gaggcgcggc tcacagcgat
      481 catcggccgg tgtcacgggc cgggtggcgc ggcgccccca ttacgctcgc ccgcgtgcct
      541 ctctacgcgc tcggcgacgt cgaacccgac attcatcccg acgcctacgt ccacccggac
      601 gccgtggtga tcggcaacgt gacgatcggg gcggaggcct cggtctggcc ctccgcggtg
      661 ctccggggcg acgacggccg gatcgagatc ggcccgcgga ccagcatcca ggacggctcg
      721 atcatccact gcactccggc cgagccgacg atcatcggcg ccgaggtgac tgtcgggcac
      781 aacgtgcaca tcgagggcgc catcatcggt gaccgcgcgc tcatctcgtc gggctcggtg
      841 gtgctcaacg gggccacgat cggcgcaggc gcggtggtcg gcgcaggcgc cgtggtctcg
      901 ccgaacgcgg aggtcccgcc gcggcggatg gcgctgggcg tcccggcgcg cgtgcgggag
      961 ggccacgagg tgccagaggg ctacttcgac cacgcggtgc agagctacat ccagcgcggg
     1021 aagcggttcc gcgccgagct gcggaggctc ggctgatggc caggccgctc gaggagatcg
     1081 tggaagccgg gtgggcacgg gcgctggcgc ccgtcgcacc ggtgatcgcc gacatggggg
     1141 agttcctgcg ctccgaactg gccgcgggtc gccgctacct gccggccggg gccaacgtgc
     1201 tccgcgcgtt cacccagccg ttcgacgacg tgcgcgtgct gatcgtcggc caggacccgt
     1261 acccgacacc tgggcacgcg atcgggctgt cgttctcggt gtcaccgcag acccgtcccg
     1321 tgccgcggtc gctggccaat atcttccgtg agtacaccga agacctcgac caccccactc
     1381 cgtcgaccgg cgacctcacg gcatggaccg agcagggcgt gctactgctc aacagggtgc
     1441 tgacggtgga gccgggcaat ccgggctcgc accgcagcaa gggctgggag accgtcaccg
     1501 agcaggcgat ccgcgccctc gtcgaccgtg acggggagcc gctcgtcgca atcctctggg
     1561 gacgggacgc gcgcaacctc gtgccgctgc tcgtggatgt gccgtgcgtc gagtcggcgc
     1621 atccgagccc catgtcggcc gaccgagggt tcttcgggtc gcgcccgttc agccgcgcga
     1681 acgcgctgct cgaggagctg ggtgccgagc cggtggactg gaagctgtcc tgatcccgcc
     1741 gccgagatga caaacgccgc ccccgccgga gcggagagcg gcgtcgccgg tgagagatag
     1801 ggcaggcagg gtgctcgcgc tcaggcgcgg gcgaccttgc cggccttcag gcaggacgtg
     1861 cagacgttga ggcgctggcg gttgccaccg ccgatgcgtg cacgcacggt ctgaatgttc
     1921 gggttccagc ggcggtgggt gcggcggtgc gagtgcgaga cggacatgcc gaagcccgga
     1981 cccttgccgc agacgtcgca gacggcagcc acgtcggaca ctcctcgggg tagacgatgg
     2041 ggggaagccg ggttcacccc ggtcgcaaga caccacagta cctgcgccac cggacggggt
     2101 gcacaccacc cggtcactac gctgtggcga gtggccccga ccctggactc agcgctgctg
     2161 accggctgga cgcgggccgc cacccaggcg ctcgaacggc atcgtgccga gatcgaccgc
     2221 atcaacgtgt tcccggtccc cgacggtgac accggtacca acctgttgct gaccatgcgc
     2281 gccgccgcag aggcggtccg ccgggtgctg cgtgagaacg gcgacgggcc ggcccgcggc
     2341 gtcgccgccg tcgccggggc acttgcacgg ggcgccctcg tcggagccag gggcaactcg
     2401 ggggtgattc tctcgcaggt gctgcgcggc gtggccgatg cggtggcggc ggtcgccgga
     2461 cccacgccgg cgggggcggt gctggccgac gcgctccgcc gggcgcaccg gctcgccacc
     2521 gccgcgttca cccgcccgcg ggagggcacc gtgctgtcgg tgctcggggc tgcggcgggt
     2581 gccgcagcgg cggcgggatc cgaccggctc gacgacgtcg cactcgcggc ggccgaggcg
     2641 gcggcggggg cggtgcaggc caccaccggg cagctgcccg agctggccag ggccggcgtc
     2701 gtcgacgcag gtggcctcgg tctctacctc gtgctcgacg cgctcgccgg gctggtgagc
     2761 gggcggacct gcacggtccg gctcccggcg acgggtaccc ctgtccgagg gcccgcgcgg
     2821 tccggggacc ttctcgtcgc cgagcgcgaa agtgggtcgg ccatctacga ctacgaggtc
     2881 atgtacctgc tcgagggtgc cggtcccgac caggtggacg ttctgcgggc cgagctgcag
     2941 gcactcgggg actgcgtctc cgttgtcggc gacggcggag aagaccgcgg cggtctgtgg
     3001 aacgtccatg tgcactgcac cgacatcggt ggggcgatcg aggcggggct ggccgccggc
     3061 aggccgcacc ggatcacggt gatccggttc gccgaccagg tcggcgacct ccccaagccc
     3121 cagcgggacg cgcggttcgt ccgcgagcgt gccgtgctga tggtcgtcgg cggctccgag
     3181 atcgccgagc tggcccggtc cgcaggcgcc gcggtgctgg agcgcccgcg cgaccgcgag
     3241 gtggacgagg ccgacctcgc cgctgcgttg gccggcaccc gagcccggca cgtggtgctg
     3301 ctccccgatg actccgagct caccgccgtg gccgagcgtg ctgcggccgt cgcgcgccgc
     3361 gacgggcagg aggtgctcgt ggtgcccacg gcgtcggtgc tgcaagggtt gtcggcgctg
     3421 gccgtgcacg accccgagcg gcgggcgggc gacgacgttg tcgcgatggc cgaggcggcc
     3481 gcgggcaccc ggaccggtgg cctcttggtg gccgagtccg aggcgctcac gtgggtgggg
     3541 cactgccagc ccggtgatgt cctcggtgtc tccgacggcg aggtcgtgct gatcgctccc
     3601 gatctcgctg tcggcggcct gtggttggct catcgcatgc tcaccgctgg tggcgaacgc
     3661 gtcaccgtgc tgctcgggga gggggccgac gaggccctcg gcgacgggct cgcagtcgac
     3721 ctgcgcctca cccatcccga ggtcgacgtc gtcgtgcacc gtggggggca gaccgactac
     3781 ccgatcgtgc ttggggtgga atgaccgacg tcgacatgct cagcccgctg ctcccgctcg
     3841 tcggtaagcg ggcggccgac ctgctctcca cgcagctcga gatccacacc gtgggcgacc
     3901 tggtgcggca ctatccccgc cgctacgtcg accgcggcaa gctcaccgac atcgccgggc
     3961 tcgagatcgg ggagcatgcc accctcgtcg cggaggtgga gaaggccacg ctccgcgaca
     4021 tgcgcgcgcg tcgcggcaag ctgctgaacg tcgtcatccg cgacgagaag ggcgcctcgc
     4081 tcgactgcac gttcttcaac ggccacaagg tccagcacgt catccgcccg ggcgtgcgcg
     4141 cggtgttctc gggcaaggtc ggggtgttca acaagaagct gcagctcacc cacccgcagt
     4201 tcgagccgct cgacgaggcc gacgagatcc gtcccttcct ctcggtctac ccggccaccg
     4261 gcaagatcaa ctcacaggag atcgcccgct gcgtccgcca ggtgctcgac ctcctcgacg
     4321 accccactga tccgctgccc gctgcgttgc ggcagcgcga gggcctggct gagctcgggc
     4381 gggccctgcg gcgcatccac gtacccgaga ccgaggccga cttccacgca gcccgcaacc
     4441 ggctcgtctg ggacgaggcg atcggcgtcc agctcgcgct ggcgctgcgc cgtcaggcca
     4501 cggtctcgcg gcccgcagcg gcatgcccgc caaagcccgg cggcctgctc gacgccttcg
     4561 acgcggcgct gccgttcacg ctcaccgcgg gccagcacgc cgtgggcgcg gagatctccg
     4621 ccgacctcgc gcgcgagcac ccgatgaacc gcctcgtgca gggcgacgtc ggggcgggca
     4681 agaccgtgat cgcgctacgg gcgatgctcc aggtcgtcga cggcgggcgt caggcggcca
     4741 tgctcgcgcc caccgaggtg ctcgccgctc agcacgcccg gtcgttgcgc accctgctcg
     4801 gcccgctcgg ccaggcgggc gagctcggcg gcgctgagca gtccatcggc gtcacgctgc
     4861 tcaccgggtc gctcggcgcg aaggcgcggc ggcaggcgct gctcgacgcg cagtccgggg
     4921 cagcggggat cgtcgtcggc acgcacgcgc tgatccagga tcacgtcggg ttcgccgacc
     4981 tcggcctggt cgtcgtcgac gagcagcacc ggttcggtgt cgagcagcgc gacgcgctcc
     5041 gcgggcgcgg ggaggtggcc ccacacatgt tggtgatgac cgccacaccg atcccgcgca
     5101 ccgtcgcgat gacggtgtac ggcgacctgg aggtctccgc gctgcgtgag ctgccgggcg
     5161 ggcgctcgcc catcgccacc accgtcgtcc cgttggcgga gaagcccggc tggctgcagc
     5221 aggtctggcg ccgcgtgcgc gaggaggccg cggccgggca ccaggtttat gtggtgtgcc
     5281 cgcgcgtcgg cggcgacccg gcgaagggcg atcccgacgc cgacctcgtc gatctcgacg
     5341 accccgacat gggtgccgac gcggacgacg catccgcccg caggcccccg ctcgcggtgc
     5401 tcgaggtgct gcccaagctc gccgaggggg cgctgcacgg gctgcggctc ggtgttctgc
     5461 acgggaagct gccgcccgac gagaaggacg ccgtgatgcg ggccttcgag cgcggcgagc
     5521 tggacgtcat ggtcgccacc accgtcatcg aggtcggcgt cgacgtaccg aacgccaccg
     5581 gcatggtgat cctcgacgcc gaacgcttcg gcctctccca gctccaccag ctccgcgggc
     5641 gggtgggccg gggctccgct cccggcgtct gccttctggt caccgaggcg ccggaggcga
     5701 gcacggcgcg ggagcggctc gacgcggtcg ccggcaccac cgacggcttc gagctcgccc
     5761 ggctcgacct cgaactacgc cgggagggcg acatcctggg cgctctgcag tccgggcgac
     5821 ggtccggtct gcggctgctg tcgctgcttc ggcacgaaga gatcatcgcg aaggcgcgag
     5881 tctacgcggc ccacatcgtc gccgacgacc ccggcctccg ccgccacccc ggcctcgccg
     5941 ctctcgtcgg cgaaacggtg ggcgacgagg aacgtgcggc ctacctggac aaggtctgac
     6001 cgctgcgcac tcgacgcgtc aggcggggag ggcaccccgg gcggcggcat cgagggctgc
     6061 tccagcggag tgggggaggt ggatcacccc ggcgcggtcg agtttggcga gttcggagcg
     6121 ggcccgggcg taggcggcga ggcgctcagg gtcgctgtcg gagtcgcggg cggtggtgag
     6181 cagcttgatg atgcgctcga ccgtgccgcg ctcggcgggg gacagccccg agagccggat
     6241 gcgttccgcg gcatcgcggg ccgcgtgcca ggctcgatca gccctctcga cggcggcgac
     6301 gaacttcgcg gcgtgcttgc cgggcgggaa cttgtcggag tcgagggctt gggcctcggc
     6361 gaaggcgtcg acgaaccgtg cggtgctggg cacggacacg tcggcgaggg cgggtaggcg
     6421 cagcacctcc atcgggtcgc actcgaacgc tgcgtacgcc gagcgcagtg tctggaaccg
     6481 ggtgcgggcg tcgttccagg ccacctcggg ccgggcctcg ttacgcacgg ggtgcggcgc
     6541 gtgggcaggg ctgcgccgct gcagcgcggt gtaccgcctg tgccggtgca cggcgacgaa
     6601 cgcgatcgcg cccgtcatca ccatcggggc tgcgccggcc atgatcgcca cgccgaagag
     6661 gatcaggctg accatcacca tcgcgagtgc gcttgctccg ccggcaacca gcgggacggc
     6721 gagcagcaac agccacggcg gctgggggtt gcgccggtgt ccgtggtggc ccgcgacgtg
     6781 acgccgcctg cgccgcatct cggccgcttc acggaggcgg cgcccctccg cgaacgcggc
     6841 ggactggccc gcctgaaccc gtggaatcac gtcgcaccct tcgtcgtcac gaagccggcc
     6901 gctgacccgg ccgagacggc ggcgcgcatc cgccccgcga gagtatcgcg gtgcggatgg
     6961 tgtcgcaccc ctccttatta ccgaagcgcg gtgcggttcg cacgggtccg atcgagtgcc
     7021 cgcaggtacg gttcgcttcc ggccgggtca gcagggaagg ggttgccgtg ttccgcaagg
     7081 tgctcgtcgc gaaccggggg gagatcgcca tcagggcttt ccgggcggcc tacgagctcg
     7141 gcgtctcgac cgtggcgatc ttcccctacg aggaccgcaa ctccctccac cgcgcgaagg
     7201 ccgacgagtc ctaccagata ggcgaggagg gccaccccgt ccgggcctac ctgtcggtgg
     7261 acgagatcat cgcggcggct cgccgtgccg gagccgacgc catctacccc gggtacggat
     7321 tcctgtccga gaaccccgag ctggcggagg cgtgcgcaca ggcgggcatc acgttcgtcg
     7381 ggcccccggc cgacgtcctg catctgacgg gcaacaaggc ccgcgccgtc gccgccgctc
     7441 gcgaggcggg cgtggccgtg ctcgactcgt cggagccgtc cgacgacgtc gacacgctcg
     7501 tcgccgcggc cgaggatgtc gggttcccga tcttcgtgaa ggccgtcgcc ggtggcggag
     7561 ggcgcgggat gcggcgcgtc gaggagccgg gcgacctccg cgagtccatc gaggcggcga
     7621 tgcgggaggc ggagtcggcc ttcggcgacg cgacggtgtt cctcgagcag gccgtcgtga
     7681 accctcgcca catcgaggtg cagatcctcg ccgacgccga cgccgagggc gaggtcgtgc
     7741 acctctacga gcgcgactgc tcggtgcagc gccgtcacca gaaggtcatc gagatcgcgc
     7801 cggcgccgaa cctcgacccg gagctgcgcg acacgatctg tgccgacgcc gtggcgttcg
     7861 cgcgtcacat cggctacgtc aatgccggca cggtcgagtt cctgctcgac gagcgcggcc
     7921 accacgtgtt catcgagatg aacccgcgca tccaggtgga gcacacggtc accgaggagg
     7981 tcaccgaccg cgacctggtg atcgcgcagc tgcgcatcgc ggccggtgcg actttggcgg
     8041 gcctggggat gcggcaggag gacatccacg tccacggtgc cgcgctgcag tgccggatca
     8101 ccaccgagga cccgtcgaac ggcttccgcc cggacaccgg cgtgatcggc gcctaccgtt
     8161 ccccgggcgg agcgggtgtc cggctcgacg gcgggacgac gcacaccggc gccgaggtga
     8221 gcgcacactt cgactcgatg ctggtgaagc tcacctgccg cggccgcacc ttccgcaccg
     8281 ccgtgcgccg ggcgaagcgg gccgtggccg agttccgcat ccgcggtgtg tccaccaaca
     8341 tcccgttcct ggcagcggtg ctcgccgacc ccgacttcca ggagggccgg atcaccacga
     8401 gcttcatcga cgagcgtccg cagctgctga cggctcgcca gcccgcggac cgcggcaccc
     8461 ggctgctcac ctatctggcc gagaccaccg tgaaccggcc caacgggacg cggcccaagg
     8521 gggtcgagcc ggtggacaag ctgccggtct gcgacctggg cccagccgcc ccggacgggt
     8581 ctcgccagct cctgcgcgag ctcggtcccg agggatttgc ctcgtggctg cgtggacggg
     8641 acgccgtggc cgtcaccgac acgaccttcc gcgacgcgca ccagtcgctg ctggcaacgc
     8701 gcgtacgcac ccgcgacctg ctcgccgtcg ccccgtatgt cgcgcggacc atgccacagc
     8761 tgttcagcct ggagtgctgg ggcggtgcga cctacgacgt cgcgctgcgg ttcctcgccg
     8821 aggacccgtg ggagcgcctc gcggcactgc gcgaggcggt gcccaacatc tgcacgcaga
     8881 tgctgctgcg gggacgcaac accgtcggct acacgcccta cccgacggag gtgaccgacg
     8941 ccttcgtcga ggaggcggcc cgcaccggga tggacatctt ccggatcttc gacgcgctca
     9001 acgacgtcga gcagatgcgc ccggcgatcg aggcggtccg gaggaccggg acaacggtcg
     9061 ccgaggtcgc actgtgctac accggcgacc tctccgaccc cgccgagccg ctgtacacgc
     9121 tggactacta cctccggctg gccgagcaga tcgtcgatgc cggcgcgcac gtgctggcga
     9181 tcaaggacat ggccgggctg ttgcgcccgc ccgccgcccg caccctggtg acggcgctgc
     9241 gcgagcggtt cgacctgccg gtgcacctgc acacccacga caccgcgggc ggacagctcg
     9301 ccacgctcgt cgccgcgatc gaggccgggg tcgacgcggt cgacgcggcc gtggcgtcga
     9361 tggcagggac gacgagccag cccgcgctgt cgtcgctggt cgcagccacc gaccacacgg
     9421 cgcgtgccac cgggctcgac ctgcaggcct gctgcgactt cgagccgtac tgggaggcgg
     9481 tgcgcaaggt ctacgcgccg ttcgaatcgg gcctcgcatc cccgaccggg cgcgtctacc
     9541 accacgagat ccccggaggg cagctgtcga acctgcgcca gcaggccatc gcgctggggc
     9601 tcggcgaccg cttcgagctg atcgaggact gctacgccgc ggccgaccgg atgctgggca
     9661 ggctggtgaa ggtgacaccg tcgtcgaagg tggtgggcga cctcgcgctg cacctcgtcg
     9721 gcgccggcgt cgagcccaag gacttcgagg ccgcaccggg tgacttcgac gtgcccgact
     9781 ccgtgatcgg gttcctccgc ggcgagctcg gggacccgcc cggcggctgg ccggagccgt
     9841 tccgcacgcg ggcgctggcg ggtcggtcgg ccgagaagga ggcggtcgac ctgtcgatcg
     9901 ccgaccgccg agcgttgcgc gacgaccgcc gcccggcgtt gaaccggctg ctgttcgagg
     9961 agccggcgaa ggggtacgtg acgcacctgg agacctacgg cgacacatcc gtgctgtcca
    10021 cgaaggactt cctgtacggg ctcgagccgg agctcgagca cacggtcgac ctggagccgg
    10081 gcgtgacgct gctgatcgag ctggaggcga tctccgagcc cgacgagcgc ggctaccgaa
    10141 atgtgctcgc cacgctcaac ggccagatgc ggcccgtgtc ggtgcgcgac catgcggtgg
    10201 ccagcgacgt caaggcggcg gagaaggccg accggtccaa cccgcagcac gtcgccgcgc
    10261 cgttcgccgg tgtcgtcacg ctgtcggtgg acgagggcga ctccgtggag tccggccaga
    10321 cggtggcgac gatcgaggcg atgaagatgg aggcgtcgat caccgcgcat caggcgggca
    10381 cggtcgcgag gctcgccatc ggcgaggttc agcaggtaga aggcggcgac ctgctcctgg
    10441 agctgtcgtg agaggccgtg cgcggatacg ggtgctctgg cactcgtatc cgcgcacggg
    10501 cacgaagtgc gcatagtggc cggcgcggca ggcggccgaa ggctgctggt gcccccgaag
    10561 ggcacgaggc cgacctcgga ccgggtgcgt gaggcgctgt tcagcgcact ggagaacgac
    10621 ccggggatcg agggcgccgc cgtgctcgac ctctgcgccg ggtcgggcgc cctcgggctg
    10681 gaggcgctct cccgaggcgc ggaccatgct ctgttcgtcg agtccgaccg gcgggcggcc
    10741 acggttctgc gacgcaacgt cgccgccatc gggctggccg gcgcggtcgt ccgctcggcg
    10801 cctgcagcta ccgttctcgg tgcggcggct gaccgggcct acgacatcgt gctcgtcgac
    10861 ccgccctacg gcgtgccgga cgccgaggtc gccggctggc tcaccgcggc cgcggcacac
    10921 ggatggctgg ccgatgccgc cacggtcgtg gtggagcggg cttcgcgcgg cggctcgttt
    10981 ccctggccgg agccgttgcg aggcgtgcgt gagcgtcggt acggggagac ggcgctgcac
    11041 gtcgccctcc ggtagcgtcc gggttgtgag gttcccgaga tgaggcgcgc cgtatgcccc
    11101 ggctcgttcg acccaccgac caacggacat ctcgatgtca tcagccgtgc gtcggccctg
    11161 ttcgacgagg tcgtcgtcgc ggtgctggtc aacaagagca agaagagcat gttcagcgtc
    11221 gacgagcgca tggcgatgct cgccgagatc gccgtgccgt acccgaacgt acgcgtcggg
    11281 tccttccacg gcctcctggt ggactactgc cgggacaatg acatccgggc catcgtcaag
    11341 ggcctgcgag cggtcaccga cttcgactac gagctgcaga tggcgcagat gaaccagcgg
    11401 ctgtccggtg tggacacgct gttcatgtcg accagccccg actacagctt cgtgtcgagc
    11461 tcgctgatca aggaggtggc gacctacggc ggcgacgtcg cccacctcct gccggagtcg
    11521 gtacatcgcc gtctcctcga gcggatcgcc gaacgttcct gagtcacccg ttcgggaact
    11581 tgacacgcgg gagcgcgggc aattcgagcc gcggcggtgt cgcggtgaga ggatgccccc
    11641 gtgtaccggg tcttcgaatc gctcgacgcg ctggtcacgg tcgtcgagga ggctcggggt
    11701 gtcccgatga cctcgaactg cgtcgttccc cgcggtgacg tgctcgagct gctcgacgac
    11761 gtccgggagg cgctgcccgg cgagctggac gatgcccagg acgtgctcga ccggcgtgac
    11821 gagctggtgg acgaggcggc acaggaggcc gagcagacgc gctccggtgc gcaggccgac
    11881 gccgcggaga tgctcgccga ggcgcgcgcg gaggccgagc gcatggtcgg ggaggcccgc
    11941 gcggaggcgg agcagaccct cgcgcaggcc cggcacgatg cggagcgggc cgtcacggag
    12001 ggccgccgcc aatacacgga gctgaccgac cgggcccagg ccgaggcaga tcggctcgcc
    12061 catgccggcc gcgccgcaca cgaccgctac atcgcggacg gtcaggccga gcaggcccgg
    12121 ctggtgtcgc agaccgaggt ggtgcaggcg gcccacgccg aggcggcccg gctcgtcgac
    12181 ggcgccgaga gggaggtcga ccggctgcgc cgcgaatgcg acaactacgt cgacgcgaag
    12241 ctggccgagt tcgaggacgc cctcgggaag gccctgcgca cgatctcccg gggccgcacc
    12301 cagatgtggc ggggcggttc acccaacagc gccccggccg ggatcccgaa cggtcgttcg
    12361 ggcaccggta tggacctcat cgactgaggc gacatcgccc cggatcacct cttgatcacc
    12421 atcgggtgcc taccgtcttc gtcgcgttac tctctgccag tggcacgtgg ccaggtcgaa
    12481 cctgatccgt ccatctggtg ggagcggtac ggccaccggt tggaaccgcg ctcgggggtc
    12541 acgaccacgg accggcgacg tgcgggagcg ggtccgcagc cagggggtgc agtgagcgcg
    12601 acgggagatc catccccggt cggtccggtg ctgggggacg ggacgccgac gacggcctcg
    12661 gagctgagct cggaggccat tctgcgggcc agaccggacg agccggcctc gggctggcgc
    12721 cgcgccgtcc tccgcgccac cgggggcctg gtgaacccgg ggccgagccc cgccgaggtg
    12781 cagtggcggg atcagctcca tcgcatccgc cacccgctcc tgcagtccca ccggatcgcg
    12841 gtgacgtcga tcaagggcgg ggtaggcaag accaccgtgg tcacgctgtt gggtcttgtc
    12901 atggccgaga accgcggcga ccgggtgatc gtcctcgacg ccaaccccga tgccgggacg
    12961 ctggccgacc gcctcaccgg cgagtcgtcg gtcaccgtcc gcgagctgct ccgtgacctc
    13021 caccgcatcc actcctggac cgaggtctcc cgctacacca gcctggccgg acgcctgcag
    13081 gtgctggcat ccgagcagga cccggcgtcc agcgacgcgt tcagccgcga ggagtacgag
    13141 cacatctgcg gattactcga ccgcttcttc aacatcatca tcacggactc gggcaccggc
    13201 ctcgtgcact ccgcgatgga gggcaccctc aagctcgccg acagcctcat cgtcgtcggg
    13261 gcgcccaccg tggacggggc gggccgtgcg agcaagaccc tcgactggct gcacgcgcac
    13321 gagcacaccg cgctcgcgtc ggaggccgtg gtcgtgctgt cctgcgaccg ggtcagcgcg
    13381 gaggtcgatc gcgagcggat ccgcgagcac ttcaccgccc ggtgccgcgc cgtcatcgag
    13441 ctaccgcacg acccgcatct cgcgacgggc ggccgggtgg agatcgcccg gctgcggccc
    13501 gccacccgcg acgcggcctt ccagctcgcg gcactcatgg ccgaccgctt cgtcctctga
    13561 gcgcggtcgc cacgctgtcg cgatgagtct gcggcgatca actacagcgg tactcttcgg
    13621 acattcgcga tgatcgggcg cagcctgtga tcagggggag tggtggccgt gagcggccgg
    13681 gggagcaccg ccgcggcggg agcggggaac gacgaccgga gcggtacgac gggtgtgcgt
    13741 aggctgccgg ccatcgcgac gtcccgttcg gctgcgctcg ccctaccgga ggtggcacgg
    13801 ggatgacgca gccgcgcgag gcgaccggcg gcacccgggc cgacgccgca cccgacgagc
    13861 cgaaagcggc ccccgccgag gggccggtcg ccgccaccac gtccgccgga tcgcccgacc
    13921 actccggcgg cagctccacc gactccgatg agttcgctgc acgtccgggg gcggagcccc
    13981 cgaccacgcc ccgctcccgc cccgaagccg ccagcgccgc ggagctcacc gaggacgcca
    14041 tcgtgcgctt ccgcagcgat cgtccccagc gcggttggcg gggggcgctc tacaagatca
    14101 ccggcggggt ggtgaacccc ggtgtgggtg cggccgagcg ggcccggcag gaccagctcc
    14161 gccgaatccg acgcccgttg cccggctccc accagatcgc ggtcagctcg ctcaagggag
    14221 gggtcggcaa gaccacggtc tcggcgtgcc tggggctcgt gctggcggag aaccggggcg
    14281 accgggtgat cgctctcgat gccaatcccg acgccggcac actggccgac cgtctcacca
    14341 ccgagacgtc ggtgacggtc cggaacctgc tcgacaacat ccacgccacg cactcgctca
    14401 ccgacatcgc gcgctacacc agccttgccg gcaggctgca ggttctcgcg tcggagcagg
    14461 acccggcgat gagcgaggcg ttcagccgcg acgagtacga gcaggtctgc gaggtgctca
    14521 cccgcttcta caacatcgtg atcaccgact cgggcacggg cctggtgcat tcggcgatgg
    14581 agggcacgct ggcgctggcc gacagcctgg tggtggtcgg tgccccgacc gtcgacggcg
    14641 cgagcagggc cagcaagact ctcgactggt tgattgcgca cggccacgcc acgcaggtgg
    14701 cgaacgcagt cgtggtgctc agctgcgacc ggatgagtaa ggaggtcgac ctggagcggg
    14761 tcagggggca cttcgcggcc cgctgtcgcg ccgtggtcga gatcccgcac gacccgcacc
    14821 tcgccaccgg cggccggatc gagctcgcac ggttgcggcc ggccacccac gacgccttcc
    14881 tgaccctcgc cgcactgctt gccgatgcct tcgactcctc caaggctccc gtccggcagg
    14941 tcgcgcctga gcaggtgccg agcctgagct gagcgagcac gccaggcgac ctgccctgga
    15001 cgtgcctgcc cgcgctgggc ggctgcacgc cggcggcgga cgcccggggg cagagttcgt
    15061 gaccacacga ggatgccggg ccgtgccgca ctgatctgcg gcacagcccg gcatgggtcg
    15121 tatcgatcag cccctgctgg agctactggg cttggagctg tcgctcttgg agctgcctgg
    15181 cttcgagctg tcgcccttcg agctgtcgcc cttggagccg ctggccttgg agctgccgcc
    15241 cttcgagctg ccggcgtcgg agctgttggt tctggtggtc ttcttgccgg tggccgtgtc
    15301 gccgccgctg tcggagctgt tgatcctggt cttcttgccg gtcggcctgc tgccgctgtc
    15361 ggagccggtg ggcgtggtcg cgccggcctt gctgccgtcc gacgtccggt ccttggcctt
    15421 gctgctggtg ttctggtggc ttgcggagcc ggggttggtg gccctcgtgc tcgtcctgcc
    15481 ctcacccgtg ctgcctgctg ccgtgccacg atcgttcctc gaggccgtgg acgtgccgga
    15541 cctgctcttc acgcccgggg ccgtcgtgcc ggccttcgaa ccgcgttcct tcgccggagc
    15601 gagcaccccc gccaccgcgc ggtctgcagc ggctttcgcc gcggccttgg cgcgttcggc
    15661 cttgccgcgc ggcttgctcg tggagctcac cttgcggtca ccgcctgccg actgctgcgt
    15721 ctcctggcgt cgatcagcct gcgaaccggg gtccgcgctc ccgccccgtt cggactttcc
    15781 gaccgacccg gaccgcctcg acgagtcggc gttcgaggag ctggagctgc gctggtcgtc
    15841 tgacctgccg agcaccaaat cggagaccgt gttgacgacc ttgctgactg gcctgacccg
    15901 ctcctcggac cgctccgagc ggtcgtcgtc gttcgactgt ccgttcgtgg cggccttctc
    15961 ggcggtatcc acgacgtgtc cggcggtgcg gatgacggca ccgctcgtgc ggaccgcgtt
    16021 ggtcacggct ccgttggtca cggctccgtt ggtcacggcc tcgttggtga cggccccgtt
    16081 ggtcacggcg ccgctcgtcc ggcccctgct ctccgtggcg ttgccgtcct ggccgcggtc
    16141 gacgcgctgt gcgacgacaa ggccgccggc cggccgggac gggtcggggg gcgccgtgga
    16201 gctgatggtc tgccgttcct cgcgcgtgct ctcgcggcgg ggagcgatcg ccggtgcctg
    16261 ctcgcgcgct tccgcctggg ggcgcgggtc gggccgatgg tccatcagcg ccaggacccg
    16321 ctcgacgatc ggtgtggtct gatcggtctc gtccggtagc ggcgcggcga gcgcctcctg
    16381 gacaccgctg cggagggtgg cgagctcctg catgttgtag tcgcgctgct ggaggtgcag
    16441 gtcgcgctgc gccgccaggt tccggacagc ctcggcctgg tcgagagagc tcgtctgctc
    16501 cggggcggtg cgcagggccc gctccacggc gtcgagctgg ccctgcgccg cagcgaggtc
    16561 ggtggtgacg tccgacaggt tccggaaggc cccgatctgc gactgcaacg tgacccgctg
    16621 ctgttccagg agcgccttcc gctcctggag ggcacgcaag gcgcccggct gcccgcttcg
    16681 ctggtgttcg ggcagccggg tccagctcgc ttcggtgagc gcgacgatgt cgagctgctt
    16741 gttgatcgac gcgaggacga tctcggcgtg ggcgacccac tggtcggcgt tcgcggtggg
    16801 cggaggcacg gacggcggct gtgcctgcgg cgtgaccggc accggggtgc tcgtcaggag
    16861 gtagccgccg cctccggcga tcagactggc aaccgcgacg ctggcgactg ctcgtttgcg
    16921 aagcagcgag gccgcagcct tgcgctcacc cgcgcttcgc gcagcgaagc ggttctccgg
    16981 gcgcggatgc tcgggcttcg gcggtggtgg cggcgtcggt gtgatgaccc agctcgcggc
    17041 ggcgctccac gggctcacgg agcgaggggg cggcggaacc gcctcgggcg tcgcgtccgc
    17101 cgtgacgggg accgtgagtg ccgcgggggc cgccaccgtc gggacctcgg gctccgcgtt
    17161 gttgtgggcc gccagatggc gctgcttccg ggcagcagcc cgatgggcgg gcgtgcagta
    17221 ccgacgcgga ggcccgccgc cgtcgggctg ctcgatggtc tcgctgcacc ccggtagggc
    17281 acacgtgttc ggctcgtcgg tcatctcgtc cccccaggag aggtcaccta tgtgtcgtgt
    17341 tcagggcctc gccataacgc ggccaggagt tggcgccgtc aggcactgct caaccgccac
    17401 ctgcccccgt gcatctccgc cgctgcccag gcagacgccg tcgcaggcgg atgggcgtac
    17461 ggagcgcacg gctgcgattg tcgttgatcg cactgccgtg ctgatgccag cacgatgcct
    17521 gaatggggag cagtggacga cattgaccgt tgtgcccgga tcggcgcgca tcgtcacgaa
    17581 cgacgactcg gcggaaccgc aatcggcgcg gacgtcgcga tccaatcgcc ggaccatcag
    17641 gatcgcagca ccgtctgctc gcgcaatgtt gcgcccgatc ggtcgtggcg acgtgctggg
    17701 tcgtgggccg cgcgtccttc ggcctcgatc aggcgttgcc tcacggcgtc gatctgggcc
    17761 ggcgagtcct ccagcgtcag gcgctgcgcg gcgaaagcgc gggtcaggtc gtgcaactga
    17821 tacccgccgt cggcgcgccg atccagcagg ccggcctcgg ccagctggtc cagaccggtc
    17881 gccgtggccg cgatcgggct ctccgtcagg gccgcgacca ccgccagggc gacccgatca
    17941 cccggcacga ggctgatcag tcgtagcagc cgctgctgct cggcgtccag ctgcacgtag
    18001 gacgcctcga gcgcgctggc cacccccatc tgcccttcgc cctcgatccg tagtgccgag
    18061 accggctccc cgacccgcag ctcgtcgagg taggcggcga gcgacaggtg gggtcgcgtc
    18121 acgaggtggg cagcggcgac gcgcagtgcc agcggcagcc cggcgcagag tgcgatcagc
    18181 tcgtcggtgg cggctggatc tgccgccagc cgcttctcgc cgacgatgcc gcgcagcacc
    18241 gtgtccgcct cgtccggccg cagcatcccg accgccaccc ggtggccccc gggggagacc
    18301 agcaggccgg ccagctcgtg gcgactcgtg accaccaccc cgcaggtggg ggagccgggc
    18361 agcagcgtcc ggacctgcgc ggggctgttc acgttgtcca ggacgatgag cagccgacgt
    18421 ccggcggtca gtgagcggta cgtcgcgatc gcgtcgtcca gtcgtcgcgg cagtccctcc
    18481 ggtggcattc cgagcgcccg taggaaatgg gccacggcct cctcggtcgt ccgcggtggg
    18541 gcggcgccgt acccgccgag gtcgacgaac agctggccgt cggggaatcg atcgacgagc
    18601 cggtgggcgg catggacggc cacggtcgtc ttccccactc ctggcggtcc tgccaccacg
    18661 accagacgtg ccgcggcgga gtttccagct cccagcaggg tcgtgatctg ctcgatctca
    18721 cggtggcgtc cgacgacgcc cgcgggtgcc atggggagct gccggacccc gtgcgagacc
    18781 gcgatcgcct gcgagccggc gggcggctcg gtccgccgca ggatctgctc gtgggcggcg
    18841 cgcagctccg ggccggggtc gatgcccagc tcgtcggcca gtagccgcgt cacctcccgg
    18901 taggcctcga gtgcctcgcc ctgccggccc gtggcgtaga gcgcccgcat ccactgcgcc
    18961 cagaaccgtt cccgcagcgg gtggctcagc acgagcccga tgagctcctc ggacagctcc
    19021 gcatggcggc cgagccggag gtcaacgtcg atccggagct ccaatgcctg cagataccgc
    19081 tcgacgaggc ggggcacctc ggtgcggtgc agggccggtg acgcgacgtt cgtcagcggc
    19141 ggtaccgccc gccacagctg cagcgcctcg gcgaggcccg ccgacgcgcc gtggtcgtca
    19201 ccctgctcgg cagcgcgcct gccctggtgg accagggcgt ccgagcggtc caggtcgagc
    19261 tgaccgggca ggagctcgat gcggtagccg tcgggctcgg tctggatggt gacgtcggtg
    19321 ccggacagag cctgccgcag acgcatcacg tacttctgca cggcgccccg gctgtcgaaa
    19381 ggggcggcac cgtcccacag ctgatcggtc agatccgtga tggacacggt ccggttggcc
    19441 cgcagcagca gtgcggcgag cacgacccgc tgcttgcccg gggttaccgg taccggtcgt
    19501 ccgccgttca cgacctccag cggtccgagg acgcgaaagg ccgcatccgt gctcaccgtc
    19561 gcccacctgc tccctgtacc ggcgcctgag atcggcgcgc cgcagccgag gcacggacag
    19621 gtcgggtgga cgacatacga tcgacacctc gcaccgtggt cgacgtcgac gccccgcccc
    19681 ggtaccgccg gcgcgcctca agtgatcacc aacaccatag gggattgttc tacaatccac
    19741 atcatgctcg tgcgccgaac ctggattgtc gtacgatcga catctcgcac gtcagttcgg
    19801 cgctccggct gcgacagctc gtactcgacg tccacggctg ctggaggccc cgaatgacca
    19861 cggacggcct ggccgcggac cggatgctgc tgaatcgcac gagcaccgcg gagcgcgtag
    19921 cggcgatcct gcgcagccgc atcagcgagg ggttcttccg gccgggtgcc cgcttggcgg
    19981 aggacaccat cgggactgcc ctcggagtct cgcgcaacac gctgcgggag gcgttccgcc
    20041 tgctctccca cgagcggctg ctcacccatg agctcaaccg cggcgtcttc gtccggacgt
    20101 tgaccgtcga ggacgtcgtc gacctgtacc gcgtgcgcgt gctcgtcgaa tgcggcgtcg
    20161 tccgggcggt cgatggggtg ccgggaaccg accggctggt ggccgccgtg acggacggcg
    20221 aggttgcgat gcggcggcag gactggcagg cgctgggtac ggcaaacctg aagttccacg
    20281 aggggcttgt ggctctcgcc gacagcccac gtgcggacga gctcatgagc ggcgtgctcg
    20341 cggagctccg gttggtgttc cacgtgatgg cggatccgag gcggttccac aagccgtacc
    20401 tggcgcgcaa ccgggggatc ctccatctac tgcagcaggg cgacgggccg gctgcggccc
    20461 gcgagctcga gctgtacctg cgggacgccg aggagcagtt ggtagcggcg taccgccacc
    20521 tgcagccacc gcccttcgag tagacctccc cctcagcgac gcgggaccgg atcacgtgcc
    20581 ggcggggtgg tgatcggccg gtgggggcgg gccccgggtt gcgtaagatc cggttggcga
    20641 tgatcacgcg ggatccaccc ggatctcgcc ttcgtgccgg acgggcgggt tcggaccagc
    20701 agtggccgcc gccgtgcggg tggggggaac atgccgaagc gagccttcgt gtcatcggac
    20761 accggtcctg ggcgagccat acccgctcca cccgagcagg gcgtgccgcc gcgccgcccc
    20821 gacgcggagg agccgacgtc gcctgcagag cttgccggtg aaccgcgcgt tcccggcgca
    20881 gcggagctca ccgacgacac gatcgttcgc cgtcgctccg acaggccgac cgccggatgg
    20941 cggcgggtcg tccacacggt cagcggcggt gtgatcaatc ccggaatcgg ctccgtcgag
    21001 cgccgtcggc agcatctgac gctgcgtatc cgccgggagc ttggccgccc gcacaagatc
    21061 gccgtgacgt cgatcaaggg cggtgtgggc aagacgacgg tcgctgcgct gttggggctg
    21121 acgctcgccg agcaccgcgg cgatcgggtt gtggcgctcg atgcgaatcc cgacgcggga
    21181 acgctggccg atcggctgac cggcgagccc ggcgcgaccg tgcgcgacct gctcgaccac
    21241 atcgacggcg tcgacgcact tcccgacgtc gcacgtttca caagcctcgc cggccggctg
    21301 caggtgctgg cgtccgagca ggacccggct cgcagcgagg ccttccaccg cgcggagtac
    21361 gagcaggtgt gcgcggtgct cacccggttc tacaacatcg tcatcacaga ctccggcacc
    21421 ggcatggtgc actccgcgat ggacggcacc ctcgccgagg ccgacactct ggtggtggtg
    21481 ggtgcgctga ccctcgacag cgccagccgg gccagcaaga cgctggactg gctggtggcc
    21541 cacgggcatc gagacctggt ctcccgtgcg gtcgtggtgc tctccgccga ccggacgagc
    21601 aaggagctgg acacggcgcg gctgcgctcg cacttctcgg tgcggtgccg cgcggtggtg
    21661 gatgttccgc atgatccgca tctggccgcc ggaggccgga tcgacatggg cgccctgcga
    21721 cccgcgaccg cggacgcgat cctcgaactg accgcgctgg tggccgacga gttcggaagt
    21781 tcgcccgccg cgcgactgcc ctgacgatct tccgtcccgg accaccggga agcgcccctc
    21841 gccttccggc cgcatcgacg gcacgatccc cggggtgagg atcttgctga cgcgagcgcg
    21901 tctcgtcctg accgttgccc tggcggcacc ggcgctgctg gtcaccgggt gcagcgcgac
    21961 tgtccccggc tcggcggcgc ctcaggcggg cggagcttcg gcgccaccct cgccgacgtc
    22021 gtcgccgagg gtggcgccgc cgtcaccggc accggtgtcg ccgaccgacg gatgcacggt
    22081 gtccgtatcg ggtccgggtc ggctcagcat gaccggcagc ggccgcgcat cgtccacgca
    22141 aggctcgatc tcgttctcgt gcggcacggg gccactcgtg gcgatcacag cgattcgcga
    22201 cggcggagtg tccttctccg cggacggagc agagacggcg gtgacgaaag gttcgatggc
    22261 cgcggtcggt ccgtatcaga tctcggtgat cgacgccgct ggtgatgccg cccgtttcca
    22321 ggtggtcccg cccgactgag agccggacgc aaccggacgg cgtaggcccg gcgtcggcga
    22381 cgaatcgcac gagcccggag cgtacgaagc ggtggagccg cccgggtcca tcccggacgg
    22441 ctccacaaac gattccgcgc cctaccgcac ccggcccgcg cccttccggc cgctgaccca
    22501 ctcctcgtcg gtcatccgca gcgccgcgac gccgaccagg atcaggaaca acccgagacc
    22561 ggccccggag atgatgttgg gaagggtgcc gaagggcacg accatgggga gcacggtgcc
    22621 gaccaccagc gggacggcgc cgagccagtg gaagaacccg gcccgccagg cggagatcgc
    22681 cgcgacgagc atgccgacga acatcgcgag gatgaagggc ccgccgatga ggaagctcag
    22741 cggcagcgcc tgcgcggcgt cggagacccg gaccgcgtcc tccggtggca gcgactggcg
    22801 gatggcgatg tcgtagaagt cggtcaccag cagcccgggc agagcgagcg cacccaccag
    22861 cgacagggcc agcccaatgt tgccgctgac cgtccaacgc cgccgggtca tgatcccgag
    22921 cgcgagcagc gccgggacga tgcccaggta gccgtagtgc agcaggagcg cggcgatctg
    22981 gctgcggaac gggtcgacga ccagtgagtc cagatagtcc agcttttcgg gcccgacctc
    23041 ccagaccgtc gtcgcgaagc ccgcggccgt cagcgcgccg ccggccaccg cggccagccc
    23101 catcgcccgg cggcggaacc gggtggcggg cgcggtggtg aggacgcccg tgccgtcgat
    23161 ggcagccgca gcgacgggtg cgctcgggcc gaactcggtg gcggtcattc gtgttctcct
    23221 cgggtgagcg gggtggcact ggtggcgcgg agtggatgac ccggcgggag gccggtgtcc
    23281 gatgaggaga actctgctcg gcacggggtt cgaagaggac cgtgtttcct tgccgatccg
    23341 cagtgtggcc agcacccggg cgacaggtgc ggccagtgcg attgtgccgt ttacgctgtg
    23401 cgcgtgatga tcagtgtcgt actggccgag gacaacgcgc tgctccggca cgggctcgtc
    23461 cgactcgtcg aagccgatgc ggacctgacg ctggccggct ccgcgggtga cctgcccacg
    23521 ttgctggacc tggtcgagaa gcacgacccg gacgtcgtcg tgacggacat ccggatgccc
    23581 ccgacgaaca ccgacgaggg catcgcgctg gccgcgcagc tgcggaccag ccgtccccag
    23641 acgggcgtac tgctgctgag ccagtacgcc gaggccgcgt acgcgctcag attgctcgcc
    23701 aacggcaccg cgagacgggg ctatctgctc aaggagcggg tggctgacgg cgacgagctc
    23761 accgacgcga tccgacgcgt cgccgagggc ggctcggtca tcgacccgat cgtgatcgag
    23821 ggcctcgtgg ccgccaaccg ggccgcgccg tccgagctcg atgcgctgac tccccgcgag
    23881 ctcgaggtgc tcggcgagat ggcgcagggc aagagcaacg cctcgatcgc cgccgcgctg
    23941 gtgctatcgg agcgcgccat cgagaagcac acgaactcga tcttctccaa gctcggtctg
    24001 tcggaggagc gcgacctcaa ccgccgggtc agcgcggtcc tcgtctacct gcagaaccgt
    24061 taggagcgcg ctgaacaacc cggccctact gcgcggcgcc cggccggcgc cctcgccgcg
    24121 ttgtcgtcgg gcccgataca acagcggtat cggcctcctc cgccttgcga cgccaccagc
    24181 tggatcaccg ctcgctacgg gggaactttt cagcaccctt ctgcccggcc acggggttgt
    24241 ctcctggtcg aggggggtgg ggccagcaac accctgcgcg tgcctgcgag cctcctcggc
    24301 gcgatgaccc catcagcatc acactgggcg gcatgagcac gcagcgggtc gtccgggttc
    24361 tggtcgtgga cgaccagcgg ccgttccggt tcgccgcggc cgcggtgctg gcccggatgc
    24421 cgggcttcga gctcgtcggc aaggccgaca ccggtgagga cgcggtggtc gccgccgcga
    24481 gcctccgccc ggacctggtg ctcatggacg tgcggctgcc cgggatgagt gggatcgagg
    24541 cggccggact cgtcgcgacc gaggtaccgg gggtcgtggt gatcctgtgc tccacctacg
    24601 cccgcgacga cctcccgttc tccctcgacg cgccgggcgt cgccggctac ctgcacaagg
    24661 aggaattgcg cgcgccggtg ctccgcgagc tgtgggagcg gtgcaggtcg tcgacccctg
    24721 ccgcctccgg tccgaccgac gccgctcgct gaggcccccg tcagcgccct cgtcagcgcc
    24781 cctgcaatgc gacggccggc ggcgtgcgcg ccgcccggcc tgcgggaacc accgacagca
    24841 gcagcgcccc gaacagcacc gcgggcacga tccacagcag ccggggcggc accgcgacgt
    24901 ccccggccac cccggtcgcg gtggccacct gccaccacag cacccgtgcg gcgcccagcc
    24961 ccagcggcac cccgaccacc agtgcgggca ccacggtggc accggccatc accgccagcg
    25021 tcgcccgcac ctgtccgggg gtcgcaccca gcacggacag caccgccacg tcccgggcgt
    25081 gccgccgggc cgcggtgacc agtgagtgga tcatcccgga gccggcgacg gcggccagca
    25141 ccagtgcgag cagctccggc agcatcagca ggtcggccag gttgcgcacg ccgtcggggg
    25201 tctcccgttc gaagatctcc agatcccggg acagttccgc gaacagcagc cccgcctgcc
    25261 cgggcactgc ctggatgccg gcatagatga gcggcggtcc ctgcacgagc tccgacagct
    25321 ggtcgcgggt gaccaccacg ccgtcgccga gaccaccccg ctcctccgac ctggtgacca
    25381 tgatgccggt gacgacgagc cggacggttc cgcccgccgg ccgatcgaac tcgatcgagt
    25441 cgccggggat gagctgcaac cggtcggcga gtcgcggacc gagggcgatc tcgccccgca
    25501 gcaacggctc gtgcccctgg agggtcgcca cggggatcgc accctttcgg ggttcccgcg
    25561 cggtcgcctc caccatcggg ccgccgctga tctgcacgtg accggacgcg ctgacgtcga
    25621 gcgcactcac ccgcgggtcg gccaccagcc gcgcgagatc gggttcgcgg gcgtcggtga
    25681 gctgcaggtc ggccgcctgc ccgtagcgcg ccggggtatc gaccaggcgc tgcaggcttg
    25741 ccccgaacgc cagcgcggcg acaacgccgg cgaccgccag tgacgcggcg acgacggcgg
    25801 cggctgccgg caccccctgg gtgccgcggc gcccgtgcag cgcgaggcgc accccgacca
    25861 gcagcgcggg ccagcgccgg ccccacgtca ccgcggcagg cgggcgggcg ggttgggggc
    25921 gccttcgctt cctgctcccg gcgagcgcca ccgcgatcgc gaccagcagc aggaagacgg
    25981 cgacgagcac gctcccgccg aggaccgcca gatcccactg gggccggaac cccatcgcgg
    26041 gctcgaaccg ggcctgactg cccagtggtt cgagccaacc cgccgcgatc accagcccgc
    26101 caccgaccac gcctccggcc accgcgccgc cgagcgccgc gaggacccgg gagccgaccc
    26161 gttccacggc tgccatcccc agggcgaact cgacctcctg ggtgttgcgt cgtgacccgt
    26221 ggaggcgtgt caggccctgg cccaccacct gcaatccgcc gagcgccagc acagcggcga
    26281 acacgaccaa cccgatgacc agcacattct ccgcggcggt gaccgcggga tcgatggtgg
    26341 cggacggtag gtcgacgaat ggcttgaggt actgcgcggc gatggagggc gatcgggcgt
    26401 cggccgccgt ggaggcggcc acgaaggcgt cggtgaacga ctgtgcggcg ccgggctcgt
    26461 cgcgcagccg aacgaacgcg ccgatcgcac ccgcgtcggc ggcgtagcgc tgcgcgaacg
    26521 cgggactggt cagcatgttg cacagtgcgc cgctccactc cggcatgcgc ccgacgccga
    26581 cgacccgcat ccgcacctcg ggcccgtccg gctcgccgaa cccgacgtcg aacatcgaga
    26641 tctcgcgcag ggtcagcatc ttcacggtga cctcggagcc gacacctaga ccggtcatcg
    26701 cggcgatcgg ctcgctgagc agcagttcgt cgagcgagtc cggggccggc tcgcggccct
    26761 cgacgacgac gggccgcacg agatcgggcg gatcggcggg ccccgcgccg acggagacga
    26821 accgcagggc gggcccctcg atccgggcga cccaggtgtg gacgagccgg gcctgcgcca
    26881 cccccggcag cgtcggaacg gctgcggcga gctccggctg gtcggtgggt acgaggacgc
    26941 gggcatcgtc caggcccacc gcctggacca gccgcgggta ggcggtggcg gtgcgttccg
    27001 cgagcgccac cgcacccagc accatcccgc cgacgagacc gaacagcagc ccgagcgcca
    27061 gtagcccgcg ccaccccgtg cgcagttcgg cacgcgccac ggcgcgcacc gcccccagcc
    27121 ggccgcccac ctcagatcgc catcgtggat ccgttggacg cgctgtccgc accacccgcc
    27181 cggcgggggt ccacgggaat ggtcaccagg cccgtcaccg cggccgacgg gcgcagcgtg
    27241 gccgcgccga gcgggacccg gccggtgact ctcgtaccct cgcccgcggc cgagcgcagc
    27301 tcgatcgagc cgccgatggc cccgagccgg tcggccatgt tctgcaaccc ggcaccggcg
    27361 gccatgccgc cgacgtcgta cccggggccg gtgtcggcga ccgcgaaccg cagctcctca
    27421 gcggtctcct cgagggtcac ggtgaccgtc gagccggcgg catgctttgc ggagttctgc
    27481 agtgcctcga ggcagcagaa gtacaccgca gcctccgtct gctcgggatg tcggcgcaaa
    27541 ccgccgacct gcaccgagac cgcgagcggg ctgcgcatgg ccgccgcccg cagcgcctcg
    27601 ccgagtccgg cgtcgcgcag cagcggcggg tagatgccgt gcgcgagatc gcgcagcgcc
    27661 tggatggatt cgcgcacccc gtggtcgagc tcgtcgagta gctcgaggtt cgcctcggcg
    27721 gccgaaccgt cgccgccctg cgccgcgtcg tcggtgtcgc cgccgggggc cacatcctgc
    27781 agcccgtccc gcaccagccg cagccccacg gccagcgcga cgaggtgctg ctgcgccccg
    27841 tcgtgcaggt cgcgctcgat ccggcggcgt tcggcgtcag cggcgttcac caggcgagtg
    27901 cgggaggcct gcagctcggc gttgctgcgt cgcaggtcgg cgagcgtggc ctgcagggcc
    27961 tcgtcgagcg cgcggttgcg cagcacgatc gcgagccggt gcgccacctc cgcgagtgct
    28021 cgctcgtcgg cggcggtgaa cgtgtcggcg tccagtgccc gctcgatgat caccagggcg
    28081 agtactcggc caccgtgcac ggccggggcc aaccggagct ggcgcgtgtc gtgccccgtc
    28141 cccagcggcc cgggcagcag ccgcggcaac cacaaccgca gccatccggg gcccgcgaca
    28201 cccgcccgct ccagcgtgga cagttcgtcg ccctgcagcg gcgccggttc gggggccggg
    28261 ctgtgcaggg gctgcgggac caccagggtg cgggccaggc cggggccgtc ggcctcaccc
    28321 gtccagatct gcaccgagga cagccgccag tcccgccgca tcgactcggc gagccggcgc
    28381 agcaggtcgt ccacgggcgt gccgcggccg gcccgctccg cgaagtcggc gagcagttcg
    28441 tccggcgacc gctggccacc gttcagcgcc gatcgcgcgg agtgtccggc ccggcggccg
    28501 agcggcccgg cgagcgtggc caccaccagc atcgcgatca acgccgggtt caccagaacc
    28561 cgctgtgcac cgatcggcag tcggccgaac accagcagca tcgtgatcag accggcgcac
    28621 acggccacag cgaggccggc ccatgcggac gcgccgacga gcacgagggt gctcgcccgg
    28681 cgggccggcg cgctgaaacc cgcgccgatc ccgagcagca gcggtccgag cgccagcgcg
    28741 gcggtcaccg gccacgatgg accaccgccg agcagcccga cgcacacgcc gagcgccgtg
    28801 acgccaacgg tgaccatcag tccggacagg atgagcgacc gccgggcccg gcggcggggg
    28861 gctgcgctca cggtccggtc gcgtgggccg gcgcggggtc gaggatgcga ccgtcgcgca
    28921 tccgcacgat ccgctgcgcc ccagccgcga cgtcgtcgct gtgggtgacc atgaggatcg
    28981 tctgcccggc caggtgcagc cggcggaaca ggtcgagcac ctcggccgcg ccgtcggagt
    29041 ccagcgcccc ggtcggctcg tcggccagca gcagggtcgg ccggttggcc agtgcccggg
    29101 cgatcgccac ccgctggcgc tgcccaccgg acaacgccgc gggtacctcg ccggacttgt
    29161 ccagcaggcc cagggtgtcg agcaggtcgc gggcccccgc ggtcgcctcc gcgggtcggg
    29221 cgccggccac cagcgcggcc aaagcgacgt tctccagcgc ggacatgtgc tcgatcaggt
    29281 ggaagaactg gaagaccagg ccgacgtgcc ggcggcgcaa ccgggcgagc ttctcctcgc
    29341 tgagctcctc ggtccggata ccggcgagcg agatggtgcc ctcgtcgggt cggtcgagcc
    29401 cggccagcag gtgcagcagc gtcgacttgc cgcagcccga cggtcccatc agcccgacga
    29461 actcgcccgg gtgcacggtg aggtcgacgc cgcgcagcgc ccgcaccggt gccgtttcgg
    29521 cctcgtaggt cttgcgcagg ccgcggacga cgagatccgg ggcgtccgga cggctgtccg
    29581 gcaccgtccc acccgccggt gccgttgtcg ctttcccgat caccaccggc acaccctagg
    29641 aggacgctga cctgcgcggg agagccgccg acgaaaccgc aagctgttac gaccgccgga
    29701 gtggtgctag caccaccgac ggtggtcagg ccaacggccc ggcgaccagg aacgcctccg
    29761 actccacttt gtgcatccgt tcgtcgtggc cgatctggca tagtaggtcg gccggtaata
    29821 gccggccgcg agcattctcg gcaggaagcc gaaccggaac tgcgacagcc tccggccctg
    29881 cgcggccacc agctcgccga cgatcagcag atgccactgc tcgttgtcct gctgctcgcg
    29941 cgtctcgacg atccgccggt ggatgcggtg tgccaagccg gtgtcggcag cggcccggtg
    30001 ccggcgggtg agcgtgcggt aagccgcgtt ctcccagatc tggtacggga tccgggcgac
    30061 gatctcgagc acccggaact tctccagcgt gtgcgcgcgc ccatagagca ggtccatcgt
    30121 gacgaacagc aggcgtgcga acaggctgta gcgccggtgc ggggcctcca gggtcgccag
    30181 ctgctcgcgc cgcagcgcgc ggcggcccag cttcggggta cgcgccgccg tcagtcggtg
    30241 cggcgggcca ggtgcgggcc cgggaacgac gatccgccgg gccggggccc gtgccgctgg
    30301 cgcggtagag cggccatcgg ggcgccggct gacgcggtga cgacggtgct gcccggccga
    30361 ccccgggtgc gcttccctgg gcactccggg tggtgccagc aaccacccgg ggttgggtca
    30421 tctgcgccat ctggatcgtc ccggccgcgc ccggttccag gtcgtcccgc ccggctgagg
    30481 gttgggcgca acgaccgcgc ggtcgaccgc ggggccggag caggcgcacg gccgctccac
    30541 gggcctactc tggtcgcgtg agtaagcacc accccactcc cggccgcccc gatccctcca
    30601 gcccgtgggt cttcatcacc cgcgagctcg gcaggcgccc ggggaacatg cgcacctaca
    30661 cacgcaccat tccggccccc gccgagccgg cgctcggcct ggagacgatc gccgtgcccg
    30721 cgggtgagcc cgtggagctc gacgtccggc tggagtcggt caccgagggc gtctacgtct
    30781 cgggcagcgt gcacgcgtct ctcgcggggg agtgcgcccg ctgcctcgac gcggttaccg
    30841 acgcggtcga cgtcgaggtg tcggagctgt tcgcctaccc cgacagcgtc accgaggaga
    30901 ccaccgacga ggacgaggtg ccgagggtcg tcgacgagat ggtcgacgtc gagcagaccg
    30961 tgcgcgacgc cgtcgtcctg gcccttccgc tggccccgct gtgccggccc gactgccccg
    31021 gcctctgtcc ggagtgtggg gagaagtggg ccgacctcgc gcccgaccac gggcatgaga
    31081 cactggatcc tcgatgggcc gctctgcgtg agcgcctatc tgctgactga caccctcgag
    31141 tactgctagg agatctaccg tggctgttcc gaagcgccgg atgtcgcggt cgaacacgcg
    31201 ctcgcgccgg tcgcagtgga aggccactgc acccacgctc gtcgcctgct cgaaccgcgc
    31261 ctgccgggcc accaagcctc cgcacgtggc ctgccccacc tgcggcacct acaacggccg
    31321 ccaggtcgtc agcccggcct gatcgccggg agcacgggaa cggcgacgac gtagtggtgg
    31381 agcgcggccc gcgggggccc gcgggtgacc gcgggcccct tctcgcatcc ctcggtgtgg
    31441 agatggccca cgacctgctc accctcgcgc tcacccaccg gtcctacgcg tacgagaacg
    31501 gcggcctgcc caccaacgag cggctggagt tcctcggcga ctcggtgctg agcatcgtgg
    31561 tcacggagcg cctctaccgc gatcacccgg acctgcccga ggggcagctg gccaagctgc
    31621 gcgcgagcgt cgtgaacatg cacgccctcg ccggcgtggc cgccaccctc ggcgacggcg
    31681 ggctcgggac gcacctctac ctgggccgcg gtgaggagct gacgggtggc aggacgaagg
    31741 cgagcatcct ggccgacgcc accgaggcgc tgatcggcgc ggtgtacctg gagcacgggc
    31801 tggagacggc tcgtgggctg gtccaccgcc tcttcgacgg gctgctgcgc ggtgccccgc
    31861 tgctcggtgc cgggctggac tggaagacga gcctgcagga gctcacggcc gcggccgacc
    31921 tcggcgttcc cgagtacagg atcaccgagg acgggccgga ccacctcaag atcttcaccg
    31981 ccaccgctgt cgtggcgggc cgggagctcg gctcgggtga ggggcgcacg aagaaggagg
    32041 ccgagcagaa gtcggccgag ctcgcatggc gcacgctgac ggccgaggcc ggtgccggca
    32101 gccagcctgc tccgtccggc cccgcctgag cgtccacggt gcctgagctc ccggaggtcg
    32161 aggtcgtccg ccgcggcctc gccgagcacg tcctcgagcg gaagatcgcc gcggtgacgg
    32221 tcgcccaccc ccgagcggtg cgccggcacg aagcgggcgg tgacgacttc gccgcgcgtc
    32281 tgaccggccg cacgatcacc ggcgcccgca ggcgcgggaa gtacctgtgg ctcgacctgg
    32341 acggcggggc agctggcagc gcagatgcgg gctccgcatg cgctcctccc ggtgcagatg
    32401 cgggctccgc atgcgctccg cccggtgcag atgcgggctc cgcatgggct ccgcccggtg
    32461 atgccgtgct cgcccacctc ggcatgagcg gccagatgct cgtcgccgat gccgagcggc
    32521 ccgacgagaa gcacctgcgg atccggttcc gcttcgtcga cggcggctcg gagctgcggt
    32581 tcgtcgacca gcggacgttc ggcggcctgt cggtgcaccc gctggtgccg gcggcggagg
    32641 gcggtctgct gccccaaccc gtcgcacaca tcgcgcgcga cccgatggac cccgcattcg
    32701 acctcgacgc cgccgtcgcc gcgatccggc gccggcgcac cggtctcaag cgcgcgctgc
    32761 tggaccagac ggtcgtgtcg ggtatcggca acatctacgc cgacgaggcg ctctggcggg
    32821 cgcggctgca ctgggcccgc cccaccgaga cgctcacccg caagcagggg cgcgaggtgc
    32881 tggccgcggc cagcgaggtg atggcggagg cgctggcggc cggtggcacg tcgttcgacg
    32941 cgctgtacgt gaacgtcaac ggagcgtcgg gctacttcga ccgctcgctc gacgtgtacg
    33001 gccaggccga ccgggggtgc cgccgctgcg gcacgcccgt ccggcgcgac ccgttcatga
    33061 accgcagctc gttcagctgc ccgcgctgcc agccccgacc gcgtcgcgca catccctgat
    33121 ccgtcgcacc gccagtcaac ccgcgtcgcg gcgcaacagg tcctcgatcg tctccctgcg
    33181 cagcatcggc acggctgcgc cgctcgccac cgcgacgacg ggcggccgtc cgacctggtt
    33241 gtaggtcgag gagagggaga cgtggtaggc gccgctacat ggcaccgcga gcaggtcacc
    33301 cgcggcgacg tcggcgggga gcagtacgtc ctcggccagc acgtccgacg actcacagtg
    33361 ccgcccgacg atcgtcaccg gcgcggacgg gacgcttacc ggcctgccga agcgccgcac
    33421 cgggtagcgg gcgccgtaga gcgcggggcg cgggttgtca ctcatccctc cgtcgacggc
    33481 gacgaatgtg cgtcgcacac cgtgcttgat ggcggcgacc cggtacacgg tgacaccggc
    33541 gcgggcgacg agggcccggc ccggctccac ggtcagccgg ggcaccgcga tgccgaaccg
    33601 gtgacacgcg gcggcaacga cggccggcac ggtgcgcgcg aacgcggcga ggtcgacgga
    33661 ctgctcaccc ggcgcgtacg ccacggcgtg gccgccgccc aggtcgagct ggggcagctc
    33721 gatgctgtgc tcgcgggaga tctcggcgag gaacgcgacc atccgttccg cggcctcgac
    33781 gtaccgcgtg accgaccgca cctgggagcc gatgtggcag tgcaacccga ccagctccag
    33841 ctcgggatgg gcgagcacgc ggcgcacggc ctccgctgcg gctcccgtcg cgatcgacag
    33901 cccgaacttc tggtcctccg tgccggtcgt gagcccggcg tgcgttccgg cgtcgacccc
    33961 ggggacgacc cgcaggagca cctgctgccg agccgggacg agggccgcga gggtcgggat
    34021 ctcctcgatg gagtcgatca caatacggcc gacccccacg cccagcgcgg tcttcaggtc
    34081 ctcgtcggac ttcacgttgc cgtgcagcac catccgatgt cccggaaagc ctccggccgc
    34141 ggccacggcg acctcgccgc cggagcagac gtcgagggcg agtccctcct ggtcggccag
    34201 ccgggccatg gcacggcaga ggaacgcctt ggccgcgtac gcgatctcca ctccggggag
    34261 cgcctcccgg taggcccggc agcgtgagcg aacgtccgtg gcgtcgatgg cgagcaccgg
    34321 ggtaccgaac cgcgcggcca ggtgggccag gtcgactcca cccacgacca gttgcccgtc
    34381 acaggtggcc cgagtggtct ccggccagag gccgggttcc aggcggcacg acagcgacgt
    34441 gcgcaacgta gggatgagat cggcgagagt caccgtgtcc tccggaatcg ataggcggcc
    34501 gacgggccac cgagagttcc gttctcccgc tcatagcccc accacgcact gctgctcatc
    34561 gctccgttga cggcaggggg ccacgtcctg acgccgtggt gacgtcaggc gtggtgctgg
    34621 tcacagagcc tctcgccgcg cgactacgac ctgacgtcaa gaacgcgtga ggatgctggc
    34681 ggaagatcac gtgccgtcgg atgattcgcg gcgcgcgcgg cgcggttgcg ctgtgctggg
    34741 gcgaggcgcg gcgacggccg ggggaggaac aggattccga tgacagacgt cgtcagccgg
    34801 ctgaggcgcg ctccgctggc gcagggcgcg gccgaggagc gacaccggct caccgcctcc
    34861 accggccttg tggcactcgg tctggacgcc ctggcacggt gcgcctacgg cccggaatcg
    34921 atcgtgctgg cgctcgccgt cgcgggctcc gcgggtatcg gcttcaccgt gccggtcacg
    34981 ctggtgatcg tcgggctgct cgcggtgctc gtggtctgct accgccaggt gatcaacctc
    35041 tacccggacg gcggcggcac gtacaccgtc gctcgcgaca acctcgggcg ccgggccgga
    35101 ctcgtcgccg cggcgtcgct ggtggtcgac tacgtgctga acgttgccgt ctccgtcgcc
    35161 gccggcgtcg cggcgctcac gtccgcgttt cccacgctgc tgccgtacac cgtcgagctc
    35221 tgcctcgcgg tgatgctgct cgtggctgcg gtgaacatgc ggggcgtcgt ggcgggcggc
    35281 aggctgttcg cgctcccggc gctggtgttc gtcggttcga tggcggtgct gatcgtcgtc
    35341 gggctggtcc gcggcgggcc gctggcgccc ctgccggcct cggacgtggt cgtcaccccg
    35401 cagacggtcg gggtgctgct gatcctcgcc gcgttcgcca acggctgtgc cgcactgacc
    35461 ggcgtggagg cgatcgccaa cgccacgccg tcgttccgcg ctccgcgccg ggtgcgggcc
    35521 cgccgcgccg aggccgggct cggcctcgtg ctgggcacgc tcctgatcgg gctcggagtg
    35581 ctcgtccagc tgttcgacgc aggccccgtc gagggtcgca gcctgctctc gctgctggcc
    35641 gagggctcgg tcggcaccgg ggtgttctac gtggtcgtgc agctgtccac cgtcgtgctg
    35701 ctcgggctgg ccgcgaacac ctcctacggc gggctcccgg tgctggccgc gcggctggcc
    35761 gcggacggtg cgctaccgca cgtgttcggc ctgcgcgggg accgacaggt gtaccgggcc
    35821 tcgatcgtgg tgctgtcggt gctcgcgggt gccctgctgc tggtctccgg cgggcaggtg
    35881 gccgtgctcg tgccgctgtt cgcgatcggt gtgttcgtcg ggttcgcgct ctgccaggcc
    35941 gggatgctgc ggcactggtg ggtgctccgc ggcccacggt ggcagtcgcg cgccgcgctc
    36001 aacgtcctgg gtgcggtact caccggggcg gccgcgctcc tgctggccgt ggagaagttc
    36061 tcagcgggtg cgtggctgat cgtggtggtg atcccggtgc tggtgctggt gttcagcggt
    36121 gtccggaccg cgtacacccg gatcggggag gtgctcgagg tcgacgagcg cccacagcgg
    36181 ccgcggcccg tggcgtcggt ggtcgtcgtc ccggtggtcg gcatcagcag gcttgcggag
    36241 gagagcctgt ccgcagcgct gtcgatgggc gaccgcgtgg tggccgtgca cgtcgtgctc
    36301 agtggcgaga acgacgaccg cgccgctgcc gacctcctgc agcagcgctg ggcggagtgg
    36361 cgcccggacg tcacgctcgt tctgctcgag gccgtcgacg agcagggcag gccgagtcgc
    36421 gtactcggcc ccgcgatcgt ttcgtacctg cgcaccctcg ccgagccggg taccgagagg
    36481 gtgctgctgc tgatcggcga ggtccagccc gaccactggt ggcagcgcgt gctgttcaac
    36541 cggcgaggtg ccgtggtggg tcgctacgcc gagcggcaca gccaggcggt ggtgtgccgg
    36601 ctgcggttcc ggctgctgcc gcggccgaag acgtctccgg tgccgcagac ccgcggcgag
    36661 cgtgggctgg tgctgccgcg gtcctgaggg ggccggccct cgtacggtct agagccagcc
    36721 gcgtcgcttg aagatcgcgt acaggcccag gccgagcagc agcatcagca gcaccgcgaa
    36781 cgggtagccg agaggccagg tcagctcggg catgtgcgtg aagttcatcc cgtagatcga
    36841 ggcgaccagc gtgggagcga acaggatcgc cgcccacgag gagatgcgct tgacctgctc
    36901 gttctggagg tagcccgcct cggtcatccg ggccatctcc tcattctggc gctgcgcgac
    36961 gagcgcggcg ttgacctgga ggatgctgct gagcagctgc cggaaggcct cgatccgctc
    37021 catcacccga gtggcgtggt cggccacgtc gcgtagggcc ctgcgcagct cgaggtcgga
    37081 ctcgctcgcg tgctccttca gccgctcccg cagctcgacg aagatgtcgc gcagcggctc
    37141 gaccgcgcgc tggaactcga tcacctcgcg gctgagctgg tagatccgct tcgagacccc
    37201 ggggtcgccg ccgaagacct gcacctcgat ctcgtcgatg tcgacctgca ggccgtcgag
    37261 caccgggctg tagtcgtcga cgatcttgtc cagcactgcg tagagcacgg agtacgggcc
    37321 gttgtcgaga agccccggat cgctctccag acgcttgcgg acctcggcca ggtccggttc
    37381 ctccgcgtgc cggacggtga tcacgaagtc gggaccgagg aacaggtgca cctcgccgat
    37441 ctcgacgacc tcgaccgggt cgacgtagcg ggccgggcgc agcaccacga acaggacgtc
    37501 gccgtagcgc tccagcttcg gtcgctggtg cgcgttgatg gtgtcctcga cggcgaggct
    37561 gtgcaggccg aactcctcgg cgacggcctt gatctcctgc tggctgggac gcagcaggcc
    37621 gatccagcag aagctgcggc catggtcggg gcactggcgg agctcctcga aggtgtggtc
    37681 gagcgacgtg gggacggcgg cacgcttgcc gtcgatgtag atcgcattgt cgatcacgga
    37741 catgcggact cctgtcgtgc ccgagggccg cgctcggcgg tcgtcgtggg tgcgccggtg
    37801 cggggcaccg tgaccttgtg cagggtatcg ccgtaggtcg gagggctgcc gggtcgccgc
    37861 gcgtcacacg tcgcggacgg cggggaacgc gggtgggtgg acatcgcagg cgccatgctg
    37921 tgggccgacc tccggaccgc cgagcagacc ggtctacatc cccagctgtt cgacccgtcc
    37981 ccggcgggct acctgctccc ggcgaggggc gcgtcagcgt acgggtactc ggtcgcgttc
    38041 ctggtcacca cgggctcgca gttctcgggc tcatcgccgt agcgtggctc gtgcccagac
    38101 cgcccgcggc cgtcggcgac ccgacggtgc aggaaccgag cactgacgat ccgcacccac
    38161 tgcccgacct tgggcgacgt cagggcggct gaggggcggg ctcaccgccc gtggacgtcg
    38221 cgcccagcct gcatgtcgca ccacggctcc gaccagctgg gcggcgacgg cggccaccac
    38281 cacgacgcct aacgcgacga cgtccgcgcg gccgccttca agcgtgccga agcggtggag
    38341 cacgaagccg tcgtaacagg cccaggacag ggcgccggca acgacggcgc cggttgtggt
    38401 ggtgagtgcc gcgagggcca tcgccgccgc cgcggccagc acgatcccgg tcgtcgggtt
    38461 gccgtcgaac gcccaggccg cgatggcggc gacggctccg gcgggcaggc cgagaacccc
    38521 accgaccgcg ccggacagca cgggccgggc ggggcttgcg tcgcctgcag tgggtcgagg
    38581 tgcgagcaca acgacaacga tccggcggtc ggagccccac gcgcggactg ttgacgttgt
    38641 cctgacggag ctgttccgac ccctgacgcc cccgtcctgc ggacggcgta ctactgtgtc
    38701 ttgcgtagta ccgtgcggta cgcagatgag gcggacaggc gccagggcga tcggagagag
    38761 ggggcggtgt ggacacgagc cagctgctca agggggtgct cgacctggct gtgctcgcgg
    38821 tgctggaccg cgcggacggc tacggctacg aggtggtgcg cgggctgcgc gcggccggtc
    38881 tggaggaggt gggagacgcg tccgtctacg gcaccctgcg ccggctctac aacgcggggc
    38941 tgctgacctc ctacgtcgtc cccagcgagg aggggccgca ccgcaagtac tacagcgtga
    39001 acgcccccgg cagggaacgg ctgcgcgaga tggtcaagac gtggcgtgcg ttcgcggaga
    39061 cgatggaagg gctggtgggg gccgcatgaa cggggacaac gagggcacgg gccgcagcgc
    39121 gacgggcgcc ggcggaaccg gcgcgcccac cgtgaggttg ctgaagggtc cggccgagga
    39181 gtacctcgcg gggatccggt ccgcgctggc ggacctgccg gcgccggagc tggccgacat
    39241 cctcgacgac gtccgcgcgc acctggccga cctcaccgcc gagctcggca gcgaggcgaa
    39301 tcatgcgggg ctcgttgtgc ggctgggcac ccccgcggca tacgcggggg agctgcgggc
    39361 ggccgccggc tacccgcctg ctcccgcccc cgccgcggca cgcgagcggt cgcggtcggc
    39421 ggcggcgtgg ttcgcggtcg cggggctcgt tgggtcggtc ctgctggtgg cgctcggtgc
    39481 cgccgcgtcc gcgcccgagg ccgtcgtgct cgggctgctc gtcgcgctgg ccgggcttcc
    39541 gctgctcacc cgggacgacc cgggggtgcc cacggtggcc gcgctgccgc tggtgcgccg
    39601 gctcaccggc agcaggcccg ccccgggaac gacagcgcgc ggcgtcgcgg acttcctggt
    39661 gagcctgcag ccggcgtggt gggtgatccg tgccgtcgtc gctgcggcgc tggtgctggc
    39721 cgtgctcgtg agcgccgacc cggccctggt cgtgctgctg tcgctggtga cggtgccgac
    39781 gtcggtctgg ctcggccatc gcacgcgcca tgaccggcgt ctgctctggg cggtggtgcc
    39841 gctcaacgct ctcgcggcgg tgctggcgct ggacgcactg ctctcgaccg gatcgggcgt
    39901 gctggggtac tccgcgccga ccagcggcta ctcggcccag ttccaccagc cggggctgtg
    39961 gcaggacacg gaccggcaga tcctcgacat ccggcccgtc gacgccgccg gcaacccgct
    40021 gaccggggtg tacctgttcg accaggacgg gcgggcgatc gacacctccg gcagcttctc
    40081 ctgcgaggac gatctccggt ccggcgagtc ggcggcgagc gcggcccgcc cctacccgcg
    40141 cggcacgacc gactacgacc cgcgcaccgg cgactgcgtg accattccgc cagggcccct
    40201 gctcgtggca gtgccgtcgg ccaccctgca gccgacctcc gcagccagca ctacagcggc
    40261 cccgccggcc accccggccg tgccgccctc acctgtgccg tcggcagtcc cgccggcccc
    40321 gccggccccg ccggtcccgc cggtggtccc gcccaccggt tgacggtcct ggtccgtccc
    40381 acccgggtgc cgcgccgaga ggctgcggtg cctgcgggcg gcctgc
//
LOCUS       DASRSC010000049        14603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1161523,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000049 DASRSC010000000
VERSION     DASRSC010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 14603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14603
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1161523"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..790
                     /locus_tag="VFE39_02565"
     CDS             269..790
                     /locus_tag="VFE39_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6257512.1"
                     /translation="MWWPAAGTAGCGAGESLEVVNALWDSWEPGALTLDGSGNAVLDP
                     SKVHPINHSGRFFTVAGPLNIPPLPQRRPVQIQAGQSEAGMALGARFAEIVFTSLPTL
                     DIALDFTRRIRSQAARRGRPDGLPLIFSSFHATYGATEEEARRLVREKNEALDFDLGR
                     APRSGSGPVSWTC"
     gene            complement(1090..1698)
                     /locus_tag="VFE39_02570"
     CDS             complement(1090..1698)
                     /locus_tag="VFE39_02570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6257513.1"
                     /translation="MACPAALADAPPAAPTGPPVVVDVAVVGAGALGAAVAWHLARRG
                     AQVALLERCGAGDVWRAAGRAVWGEADAQPRLVDEAGVLWREIERETGASLLHTGCAG
                     RQIRAGHAVAALTAAAAAWGAVLRHREPVRSIELQGGPGARLRTRTGTLHAWRVVLAG
                     ARPAELVPPRHRTRGPVVTAAAAGSFVVPALGRVLADSAECR"
     regulatory      complement(1705..1790)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     regulatory      1982..2076
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            2468..3562
                     /locus_tag="VFE39_02575"
     CDS             2468..3562
                     /locus_tag="VFE39_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020632357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6257514.1"
                     /translation="MKFLVLTLISNGPDPVTGRTPSPNARLHRVVENAVQAEELGFDG
                     YAVGERHERPFFSSAPPVVLSHIAARTSRITLFTGVTTLSLLDPVRAYEDYATLDHLA
                     DGRLELIIGKGNGAAQAQLFHVTPEDQWDRNAEGYELFRRLWREERTTWEGPFRPSLA
                     DAETWPPPRQQQIRVWHGSATSKDSVELAARYGDPLFSANVTNPIEPYAELIAHYRER
                     WVAYGHDPVDALVGAGTAGFHLAKTSQHAVEEFRPAFAHRLALAERFGLPVVFPTVED
                     FVERSSALIGSPEQIVDKVLRYHGVFGHEAMHLHLDGDGLTPRRQREMLEQFQSDVAP
                     ALRAAVPSRPLTAPVPPDPDDARPSEGDSP"
     gene            3559..4713
                     /locus_tag="VFE39_02580"
     CDS             3559..4713
                     /locus_tag="VFE39_02580"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6257515.1"
                     /translation="MTPPLSILDLAPVPRRGTAAEALHNSIDLARVAERAGYLRYWVA
                     EHHLVPGVASSSTPVLVALIASATERIRVGSGAVQLPNTRPLQVAEQFGTVAAVHPGR
                     IDLGLGRLDLHKVLTLIRASQEAGSGKPGAPPPPPLPRVVDGLLIPSPARFAGDPSMF
                     GYLGALLGFSADDPPPDYDEQVGAILDYFAGTAKRTDGEPVTVLPAEGTDLDVWVLGS
                     SPGASAQVAAARGLPFAASYHTVPSTVLETVAAYRDAFRPSERLAAPHVMVSADVVVA
                     ETDERAAELAAPYGQWVLDIRSGRGAQPYVTPEEARARVWTEQERAAVADRVGTQFVG
                     SPATVVERLETLAGVTGADELLVTTVTTEHADRVRSTELLAQAWAERRTT"
     gene            4787..5914
                     /locus_tag="VFE39_02585"
     CDS             4787..5914
                     /locus_tag="VFE39_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6257516.1"
                     /translation="MSPFTEARMHLAVELQGAGRHPAAARWAGLDRASLLTAPYYVSL
                     VRAAARRGLDLALLPDTFADDEPVLDAVAIAARVAPVVPGIGLVPTATVTHTEPFHLS
                     KAIATLDIVSGGRAGWEPVVSRTPAEAALFGRKPAAPPDVLWREAADAVEVVVRLWDS
                     WEDDAVIRDATTGRYVDRDKLHYVDFTSEFFAVKGPSITPRPPQGHPLVVLRADEEHA
                     LPVAARWADVVRIAAPDLDAAGAAREQVRAAAAAAGRDPDDVTVLLDVEVHLAADAPR
                     ARADVAELDARAALEPSSVRVTGTATDLADLLGRAVRLGAADGVTFVPLVLPADLGAL
                     ADQAVPLLAGRGLFRTGRSGGSLRSRFGLSRPANQFAESQS"
     gene            5911..7219
                     /locus_tag="VFE39_02590"
                     /pseudo
     CDS             5911..7219
                     /locus_tag="VFE39_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677861.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
     gene            7216..8034
                     /locus_tag="VFE39_02595"
     CDS             7216..8034
                     /locus_tag="VFE39_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HET6257517.1"
                     /translation="MTSTVPGTAAMFAQDWAVWHADREEQLAAPHGWLSLTALHWLDG
                     TARAYEGLPGTWRASGDGGVELAADGDAVLDGAPVTGTVRLEPVDGQPGVLVTVGERK
                     IEVIRRTDSYALRVRHPQAITRTAFTGAPAFPADERWVVKGRFEPYAEPQRLTVGAVV
                     DGLQHFPTAVGVVRFTLDGAEHALVAFPGKGGGLSLHFHDATSGQSTYGGGRTLRLDD
                     PDADGRVTVDFNRTLNLPCAFTAHATCPLPPAGNRLTAAVEAGERTPPRPDLAP"
     gene            8067..8558
                     /locus_tag="VFE39_02600"
     CDS             8067..8558
                     /locus_tag="VFE39_02600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET6257518.1"
                     /translation="MRDVLGHFASGVVVVTAAGPDGRLGFTCQSFASLSLDPPLVSFA
                     PARTSSTWPRIREAGVFCVNVLADDQEALSAGFARSGVDKFAGVDWRRGPDDAPLLGG
                     ACAWIGCTFWREYDGGDHTIVVGQVRDLAADASRSPLLFHRGRYGLTRPDGCPQEENG
                     SPA"
     gene            8561..8803
                     /locus_tag="VFE39_02605"
     CDS             8561..8803
                     /locus_tag="VFE39_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257519.1"
                     /translation="MLGIVAAPEPGGRTATAVAGLLAGAAKVSASTSLLELSEAGANA
                     VISFACGCQNLRHGCDPLVRLGAARHDRRVGSRSVC"
     gene            10098..11252
                     /locus_tag="VFE39_02610"
     CDS             10098..11252
                     /locus_tag="VFE39_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6257520.1"
                     /translation="MTAVADLRRWRRDIPGSVAITAGRADGRTRHITYEEYAQYVERL
                     TGALHELGVGPGQVVAIQLPNWWQTSALVLACARLGAVIAPVTTTIRPHELESMLAVL
                     RASVCVTVDRWADFEHSAALADIATRLPHLRHRVVLGEHVRTGEVDFALHFEYMPWEK
                     RHGISLDDPGEDPDQVSVVTFTSGSTGSPKAVLHTFNTFYAGYSPIAAKDELGPDDRL
                     FTPHPLTHGWGLRTGILLPLLVGARSVVLDTWEPEAALDLLVRTGATVVLAAPGFLSG
                     LVAEARKQSERLPAVRRIVTGATIPDGLITELADWFGVTPQSTRGMSEVIGGTRTRLT
                     DPVDLPPRSHRAPRADLEIVRSDGQMRVDPPAALRARLQLVPGHREPRHP"
     gene            11526..12422
                     /locus_tag="VFE39_02615"
     CDS             11526..12422
                     /locus_tag="VFE39_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HET6257521.1"
                     /translation="MTQTAPHQPDAAEQQAREQAVTDEVVASFATSTSDRFREVMTSL
                     VRHLHGFARDVRLTHAEWETAVDFLTRTGQISNGRRQEFIMLSDVLGLSMLTVGINAP
                     AARGATESTVFGPFFVDDAPEVPLGGDIALGAKGTPCWVSGQVRSTDGTPLAGVRIDA
                     WEADEDGLYDTQYEGNRTAGRGWQVTGEHGEYRFWSVLPTHYPIPDDGPVGELIAAAG
                     RDPMRPAHLHFKVTAPGHRTLITHVFVAGDEYLHNDAVFGVKDSLIAEFTEHQGRTAP
                     NGTTRRWTQVRFDLVLAREEQP"
     gene            12419..13603
                     /locus_tag="VFE39_02620"
     CDS             12419..13603
                     /locus_tag="VFE39_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6257522.1"
                     /translation="MTAPTTLKAPESDYDPYTDAALLNPWPGYRDLRDAGPAVWLPKY
                     EMFALTRHASVGDALRDWSTFASAYGVMMNDPMNQVLRGNTLCSDGADHDVLRRVVIK
                     PLRPQAITELTEEISREADELVERVVKQGSFDAATDLAHHLPLTIVSKLVGLPEEGRE
                     RLLEWASALFNCIGPLNERAISAFPIVQEMVDYATTQAVRGKLKPGSWGEALHDAADR
                     GEVPLEAVPVMMVDYMGPSLDTTIFAITSAVWLFAQHPDQWDLVRENPKLIPSAVNEV
                     LRMEAPIQDFSRYTVKEFDMDGVTIPPDSRAIVFYGAANRDERQYPDPDRFDVRRSPT
                     NHMAFGTGPHVCLGLYLARLEMTSLFTALARRVRRFDLGDTERALHNILRGFTKLQVA
                     VS"
BASE COUNT         2168 a         5221 c         5115 g         2099 t
ORIGIN      
        1 ccacagcgtg ccctcgatga gggcttgatc gccatcgccc gagtcgcgtg gcacccagta
       61 cgggcaccgg tagtattcgc ctacccccga gcacgactac gcccctgcaa cctcgtcgtg
      121 aggctccggg ggcgtaggtc ggtcgtcagc ccaccttcac gaaggtctca cagccggggt
      181 tggagaacgt tgcgcgccca cccaacgccg atgccgagca ggaacgccgc gttgccgacg
      241 aagatcgtgg ggcgcttcgc caccttcgat ttggtggccg gcggcgggaa ctgcggggtg
      301 cggggcgggg gagtcgctcg aggtggtcaa cgcgctgtgg gacagctggg agcccggcgc
      361 gctgactctc gacgggagcg gcaacgccgt gcttgacccc agcaaggtgc atcccatcaa
      421 ccactcaggc cggttcttca cggtggctgg gccactgaac atcccgccgc ttccgcagcg
      481 tcgtccggtg cagatccagg cggggcagtc cgaggcaggg atggcgctgg gcgcgcgttt
      541 cgccgagatc gtcttcacct ccttgccgac gttggacatc gcgctcgact tcacccggag
      601 gatccggagc caggctgcgc ggcgcgggcg ccctgacggg ttgccgctga tcttcagctc
      661 gttccacgcc acctacggcg ctaccgagga ggaagcgcga cggctggtcc gggagaagaa
      721 tgaggcgctc gacttcgacc ttggccgggc gccgaggagc ggttccgggc cggtgtcctg
      781 gacgtgctga cccttgaaat cgcgggcaat cagcgcctcg gcttgggtgc gcaggaacgg
      841 gtcccaagta ctcgacagcg gccgggtggg gtcgatgccg gggtcggtga ggatttgcca
      901 cgtggtggat ggccgcgccc gacgctacga ggctagggcc cgaggccggg acgcggccgg
      961 gccgcagaac tggtcgaact gctcacctcg tcggcccggc cagaactgct ccaggaccat
     1021 gcgcatcacg ctggtagtga atcgcgccga acttcgtccg gcaacccctc cggctggtgg
     1081 tgtgccggtt cagcggcatt ccgcggaatc ggcaagcacc cgcccgagcg cgggcacgac
     1141 gaacgagccg gcggctgccg ccgtcacgac cgggccgcgc gtccggtggc ggggcgggac
     1201 gagctccgcc ggtcgggcac ccgccagcac cacccgccac gcgtggaggg tgccggtgcg
     1261 ggtgcgcagg cgggcgccgg ggccgccctg cagctcgatg gaccgcaccg gctcgcggtg
     1321 tcgcagcacc gccccccacg ccgcggcggc ggcggtgagg gcggcgacgg cgtgaccggc
     1381 ccggatctgg cggccggcgc accccgtgtg cagcaacgac gcgcccgtct cgcgctcgat
     1441 ctcacgccag agcactcccg cctcgtccac gagccgcggc tgcgcgtcgg cctcacccca
     1501 cacggcgcgg cccgcagccc gccagacgtc accggcgccg caccgttcga gcagcgccac
     1561 ctgcgcaccc cggcgagcaa ggtgccacgc gactgcggcg ccgagagctc ctgcccccac
     1621 aacggcgacg tcgaccacga cgggcggtcc ggtcggggca gcgggaggtg cgtcggcgag
     1681 tgcggccgga caggccatgt gaatcctcca tcgatcccgg ctgaagcacc ctgcctgcgg
     1741 ggttgctgcg gcgtcccgga gccgggtctc tcggccgctc tggatgggcg gacaacagta
     1801 ggcagattcc tgccgctgct gtcaaggaat gcccactcgg aggccgcgtc ggacatttgc
     1861 gagcggaact gtccggccat ttcagccagc ccgcgaatgg cgcacgacac acttccccgg
     1921 aaaacgcttg taaatcggct cgtcagatcc gtaccgtcgg atcgcggccg ggctctggcc
     1981 gccaccatcc agagcggctg agagacctgg ctcgacgacg ccgcagcaac cccccgcgcg
     2041 agtgggtgcg ggtgctaccg ccgggagcga tggaggtcgc cgtgccgggt ccggggagca
     2101 ccccgctgca cacgacgtgg cacgcctggc gccaccacgt cgacctgtgc cgcaccagcg
     2161 cggccctctg tccggcctga cgatcccccg aacccagagc gcgcaccgtc gacgacggtg
     2221 cggtggcccg tagaaggacc ccccatgacc acattcccct tccgagcagg tctgttcgcc
     2281 gccgccgccg tattgctgac ggcctgcggc ggtggcgccg cgcccccgcg tcgattcggg
     2341 gggtcgacgc agcccggccc accgagcagc tcttggacgc gccggggcac gaatacactc
     2401 gggagctgct cgccgccatc gcggggcagc ggcgcccgtc cctgtcctgc gcagcgaagg
     2461 agcccgcatg aagttcctcg tcctcacgct gatctcgaac gggcctgacc cggtcaccgg
     2521 ccggaccccc tcgccgaacg cgcgcctgca ccgggtcgtc gagaacgcgg tgcaggccga
     2581 ggagctgggc ttcgacggct acgcggtggg tgagcggcac gagcgcccgt tcttctcctc
     2641 ggcgccgccc gtggtgctct cccacatcgc cgcccgcacg tcgcggatca cgctgttcac
     2701 cggcgtcacg acgctctcgc tgctcgaccc ggtgcgcgcc tacgaggact acgccaccct
     2761 cgaccacctc gccgacggcc gcctggagct gatcatcggc aagggcaacg gtgccgcgca
     2821 ggcgcagctg ttccacgtca cgcctgagga ccagtgggac cgcaacgccg agggctacga
     2881 gctgttccgg cggctctggc gcgaggagcg caccacctgg gaggggccgt tccgcccgtc
     2941 gctcgccgac gccgagacgt ggccaccgcc ccggcagcag cagatccggg tctggcacgg
     3001 cagcgcgacc agcaaggact ccgtggagct ggccgcccgc tacggcgacc cgctgttctc
     3061 ggcgaacgtc accaacccga tcgagccgta cgccgagctg atcgcccact accgggagcg
     3121 ctgggtggcc tacgggcacg acccggttga cgcgctggtc ggcgcgggca cggcgggctt
     3181 ccacctggcg aagacgtcgc agcacgcggt cgaggagttc cgcccggcgt tcgcgcatcg
     3241 gctcgcgctg gcggagcggt tcgggctgcc ggtggtgttc ccgacggtcg aggacttcgt
     3301 cgagcgcagc tccgcgctga tcggcagccc ggagcagatc gtcgacaagg tgctgcgcta
     3361 ccacggggtg ttcggccacg aggccatgca cctgcacctc gacggcgacg ggctcactcc
     3421 gcggcggcag cgggagatgc tggagcagtt ccagtccgac gtcgcgcccg cgctccgcgc
     3481 cgcggtgccg agccgtccgc tcacggctcc cgtgcccccg gacccggacg acgcccggcc
     3541 gtccgagggg gattcaccgt gacgccgccg ctgtcgatcc tcgacctcgc gccagtaccc
     3601 cgccggggta cggcggccga ggcgctgcac aactcgatcg acctcgcccg cgtcgccgag
     3661 cgcgccggct acctccgcta ctgggtcgcc gagcaccacc tcgtgcctgg agtggcctcg
     3721 tcgtccactc ccgtgctggt ggcgctgatc gcctcggcca ccgagcgcat ccgcgtcggg
     3781 tcgggggcgg tgcagctgcc caacacccgg ccgctgcagg tggccgaaca gttcggcacg
     3841 gtcgccgcgg tgcaccctgg ccggatcgac ctcgggctcg gccggctcga cctgcacaag
     3901 gtgctcacgc tgatccgcgc ctcgcaggag gccggttcgg gcaagccggg ggcgccgccg
     3961 ccaccgccgc taccgagggt cgtcgacggg ctgctcatcc cctcgcccgc ccggttcgca
     4021 ggcgacccgt cgatgttcgg gtacctgggc gcgctgctcg ggttcagcgc cgacgacccg
     4081 cccccggact acgacgagca ggtcggcgcg atcctcgact acttcgcggg caccgcgaag
     4141 cgcaccgacg gggagccggt caccgtgctg cccgcggagg gcaccgacct cgacgtgtgg
     4201 gtgctcggct cgagcccggg cgcgagcgcg caggtggccg cggcgcgcgg gctgccgttc
     4261 gcggcgagct accacaccgt gccgagcacg gtgctggaga cggtcgcggc ctaccgggac
     4321 gcgttccggc cgtccgagcg gctggccgcg ccgcatgtga tggtctccgc cgacgtcgtc
     4381 gtggccgaga ccgacgagcg ggccgccgag ctggccgcgc cgtacgggca gtgggtactc
     4441 gacatccgct ccggacgcgg cgcgcagccc tacgtcacgc ccgaggaggc gcgggcgcgt
     4501 gtgtggaccg agcaggaacg cgccgccgtc gccgaccggg tgggcactca gttcgtcggc
     4561 tcgccggcca cggtggtcga acgcctggag accctcgccg gcgtgaccgg ggccgacgag
     4621 ctgctggtca cgaccgtcac caccgaacac gccgaccgcg tgcgcagcac cgagctgctc
     4681 gcgcaggcgt gggccgagag aaggacgaca tgagcgagct tgcgagcgaa tcatcggcac
     4741 agcgtccggg cgaatgccgg cgacgagcgc aagcgaggag ttgaacatga gcccgttcac
     4801 ggaggcgcgg atgcacctgg ccgtggagct tcaaggggcc gggcggcacc cggcggcggc
     4861 gcggtgggcc gggctcgacc gggcgtcgct gctcaccgcg ccctactacg tctcgctggt
     4921 ccgcgcggcg gcccgccggg gcctcgacct cgcgctgctc ccggacacgt tcgccgacga
     4981 cgagcccgtg ctcgacgccg tggccatcgc cgcgcgggtg gcgccggtcg tgccggggat
     5041 cgggctcgtg cccacggcga cggtcacgca caccgagccg ttccacctgt ccaaggcgat
     5101 cgcgaccctc gacatcgtct ccggcgggcg ggccgggtgg gagcccgtgg tctccaggac
     5161 gccggcagag gctgcgctgt tcgggcgcaa gccggccgcg ccgccggacg tcctatggcg
     5221 cgaggccgcc gacgccgtcg aggtggtggt gcggctgtgg gactcctggg aggacgacgc
     5281 cgtgatccgc gacgccacca ccggccgcta cgtcgaccgc gacaagctgc actacgtcga
     5341 cttcaccagc gagttcttcg ccgtgaaggg cccgtcgatc accccgcggc ccccgcaggg
     5401 gcacccgctg gtcgtgctgc gcgccgacga ggagcatgcg ctgccggtgg ccgcccggtg
     5461 ggcggacgtc gtgcggatcg cggcgcccga cctggacgcg gcgggcgccg cccgggagca
     5521 ggtgcgggcc gcggcggcgg ccgccgggcg cgacccggac gatgtcacgg tgctgctcga
     5581 cgtcgaggtg cacctggccg ccgacgcccc ccgcgcccgc gcggacgtcg ccgagctgga
     5641 cgcgcgcgcc gcgctggaac cgtcgtcggt gcgggtcacc ggcaccgcga ccgacctcgc
     5701 cgacctgctc ggcagggccg tccgactggg ggcggcggac ggcgtcacgt tcgtgccgct
     5761 cgtgctaccc gccgacctgg gcgccctggc cgaccaggcc gtgccgctgc tggccggtcg
     5821 gggcctgttc cgcaccggac ggtccggcgg ttcgctgcgc tcacgcttcg gcctgagccg
     5881 cccggccaac cagttcgcgg agtcacagtc gtgagcaaga aacagatcca cttcggcgcg
     5941 cactttccgg gcgtcaacaa caccacggtc tggagcgacc ccccaggccg gcagccagac
     6001 cgacttcgtg tcgttcgtcc acttcgcgca gaacgccgag cgcggcctga tggacttcgt
     6061 gttcctcgcg gaggggctgc ggctgcgcga gcaccgcggg ctcatccacg acctcgacgt
     6121 cgtcgggcgc ccggacacgt tcacggtgct caacgcgatc gccgccgtca caacccacct
     6181 cggcctcgcc ggcacgatca acaccacgtt caacgagccc tacgagctcg cgaagcagtt
     6241 cgcctcgctc gaccacctct ccgggggccg ggcggcgtgg aacatggtca ccacgtccga
     6301 cgtgttcaca ggcgagaact tccgccgcgg cggtttcctg gaccgcgccc agcgctacga
     6361 gcgggcggcc gaggtcgtcg ccgtgacgag atctctgtgg gacttccgcg gcgagccggt
     6421 cgaacaccac ggcccgcagt tcgacgtgag cggccgcttc ccgctgccgc cgggaccgca
     6481 ggggcacccg gtgctgatcc aggcgggcga ctccgccgag ggtcgcgact tcgccgccgc
     6541 caccgccgac gcgatcttca cgctgcacgg aacgttggag gccgggcagg cgttttacgc
     6601 ggacgtgaag ggcaggctcc gcaggcacgg gcgcagcgag cacgacctca aggtgctgcc
     6661 gggtgtgacg ttcgtgctcg gggacaccga cgcggaggcg caggcggcgg ccgaccacat
     6721 ccgccggcag caggtctcgc cgcccaccgc gatcgcattc ctcgagcaga tctgggggct
     6781 cgacctgacg gggtacgacc cggacggacc gctgcccgac gtcgacccgg acgtcgacaa
     6841 cgtctcgatc acccgcggcc gcgtgcggca cgccaaggac ccgcgtgctg tcgcgcagga
     6901 gtggcgcgac ttcgccgccg cgggcggcgg gttgtcgatc cgtgaggtcg tcatcgagaa
     6961 gaccggccgc cagtcgttca tcggctcgcc gaggacggtg gccgaccaga tggacctgtt
     7021 cgtccagagg aacgcctgcg acggtttcat cctcgtgccg cacctgaccc ccggcgggct
     7081 cgaccggttc gtcaccgacg tcgtgcccga gctgcaggac cgcggcagct accgcaccgc
     7141 ctaccccggc ccgacgctgc gcgaccacct cggcctgcgc cacccgcacg caccgctgac
     7201 agcacaggag agcgcatgac cagcaccgtt cccggcaccg cagccatgtt cgcgcaggac
     7261 tgggcggtct ggcacgccga ccgcgaggag cagctcgccg ccccgcacgg ctggctgagc
     7321 ctcaccgccc tccactggct cgacgggacc gcgcgcgcct acgagggcct gcccggcacg
     7381 tggcgggcga gcggcgacgg gggcgtcgag ctggccgccg acggtgacgc ggtgctcgac
     7441 ggcgctccgg tgaccggcac tgtgcgcctg gagccggtcg acgggcagcc gggcgtgctc
     7501 gtgacggtcg gcgagcggaa gatcgaggtc atccggcgca ccgacagcta cgcgctgcgc
     7561 gtgcgccacc cgcaggcgat cacccgcacc gcgttcaccg gggcgccggc gttccccgcg
     7621 gacgagcgct gggtcgtcaa gggccggttc gagccctacg ccgagccgca gcgcctcacc
     7681 gtcggcgctg tcgtggacgg gctgcagcac ttcccgacgg ccgttggcgt cgtccgcttc
     7741 acgctcgacg gcgccgagca cgcgctcgtg gcctttcccg gcaagggcgg cggcctgtcg
     7801 ctgcacttcc acgacgccac ctccgggcag agcacttacg gcggcgggcg cacgctgcgg
     7861 ctcgacgacc cggacgccga cggccgcgtg accgtggact tcaatcgcac gctcaacctg
     7921 ccgtgcgcgt tcacagcaca cgccacctgc cccctcccgc cggcggggaa ccggctgaca
     7981 gctgccgtcg aggcagggga gcggacgccg ccgcggcccg acctcgcgcc gtgaacccga
     8041 ccgacatcac ggtcgacccc gcggcgatgc gggacgtcct cgggcacttc gcgtcaggcg
     8101 tcgtcgtggt gacggcagcg gggccggacg ggcggctcgg gttcacctgc cagtcgttcg
     8161 cgtcgctgtc gctggatccc ccgctggtct cgttcgcccc ggcgcggacg tcgtcgacgt
     8221 ggccgcggat ccgcgaggcc ggcgtgttct gcgtgaatgt gcttgccgac gaccaggagg
     8281 cgctcagcgc cggcttcgcc cgctccggcg tcgacaagtt cgcgggcgtg gactggcggc
     8341 gggggcccga cgacgcgccg cttctcggcg gggcctgcgc gtggatcggc tgcacgttct
     8401 ggcgcgagta cgacgggggt gaccacacga tcgtcgtcgg gcaggtccgc gacctcgccg
     8461 ccgacgcctc ccggtcgccg ctgctgttcc accggggccg ctacggtctg acccgacccg
     8521 acggctgccc gcaggaggag aacggatcac cggcctgaac gtgctcggca tcgtcgccgc
     8581 acccgagccg ggcggccgca ccgccaccgc cgtggccggg ttgctggccg gtgcggcgaa
     8641 ggtgagtgcg tcgacctcgc tgctggagct gagcgaggcc ggggccaacg ccgtgatttc
     8701 cttcgcctgc gggtgccaga atctccgtca cggctgtgat ccgctggttc gcctcggcgc
     8761 cgcgcggcat gatcgtcggg ttggctcgcg tagcgtctgc tgaatctgat catgtgtcgc
     8821 ccggcggggt ggtttacttg ctggcaaacg gcacagtgct catcgcgtgg aatgtagtgg
     8881 ttgccgcact caacgacctc gccgagttgg gtccctccgc agtgaggaaa gtgaccccgc
     8941 aagcgtaaac ggaacggtaa acaagacgcg ccagaacctg gaccgacgcc tgggaccacg
     9001 ggtatcccgg ggcatctcgc cgaccgacgt agcctccgtg tagaaatggt gcatccagcg
     9061 ccggacgcgc cgcgggccta tacggccgct ccgctgcgcc ggcgggcccg gaggaaccga
     9121 caccacgctg tcgggtcacg gtcggtggtc gaatatcagg cacccatggg cttgcgcgag
     9181 ggcgcggtgt ggcacggctg cgcagcgaac ctgcagggca cggcccgtca ggggtcggcc
     9241 gccagcccgg ctggggggtg acgcactggt gggcctggct gctggccgcg gagctcagat
     9301 gctatgggct gccccccgcc tgcccatgat ccggtgatga gccagagctc gtagtggaaa
     9361 agccattcgg tggtccaggg cacgatggtg cgcgaggagc atggtgtcgt cccactggcc
     9421 aggcgccggt aggccaccac ccggacggta taagcgggtg gcggttgggg tgttcgcgct
     9481 cggggtccgc ccgaagatcc tcaaggacaa tgccatgcgg gcgctgcggc ccgaccaggc
     9541 gaggtcgtca ccgcgaatgg ccaccatcga cgacctcccg ggtctggtcc gcaagcggct
     9601 ggggctcgga tggtggttcc ggcggagcgg cgccgtgacg tcggtcgagc cggtcgcggg
     9661 gtccagctgg ggcagtgatc ggtccgggac tgccggagaa gtcggcgtca ccccggcgct
     9721 cgccgggcac ctcacacggc acgacatccc gaccgtgacg aattcgaaga tgaaccggat
     9781 tccgagcgtc gcaacaaatt ctaggactcg gcggattgac gactctccac ggtcggcacc
     9841 ggttctcgcc aacccgtgca caggaacgtc gatctcgcga tcgctacttg ctgcaatggc
     9901 atggtcgggg gacagctatg ggccaacgga aaaggtagga aatggacaat ggggcggcag
     9961 gagtcaatcg ggtggatcga tgccttcgat cggtcgacgg gctgggacgc gccggtcggg
    10021 acttggttcc gccactgggg ttgcggccga ctgggcatca gatccggact taccgcgcgc
    10081 agggtatatg gcgggacgtg accgcagttg ctgacctgcg gcggtggcgg cgggacattc
    10141 cgggctcggt tgcgatcacc gccggtcgtg ccgacggccg tacccgccac atcacctacg
    10201 aggaatacgc gcagtatgtc gaacggttga cgggtgccct ccacgagctc ggcgtcggac
    10261 ccgggcaggt cgtcgcgatc caactaccca actggtggca gaccagcgcc ttggtcctgg
    10321 cctgtgcccg gctcggggcg gtgatagcgc cggtcaccac cactattcgc ccccatgagc
    10381 tggaatccat gcttgccgtg ctccgggcgt cggtgtgcgt gaccgtcgac cggtgggccg
    10441 acttcgaaca ttcggcggca ttggccgata tcgcgactcg cctgccgcac ctgcggcacc
    10501 gggtcgtgct gggcgaacac gtgcgaaccg gcgaggtgga cttcgccctc catttcgagt
    10561 acatgccgtg ggagaaacgg cacggtatca gcctcgacga ccccggcgag gatcccgatc
    10621 aggtctcagt ggtcacgttc acctccggta gtaccggctc gccgaaggcc gtgctgcaca
    10681 cgttcaacac cttctacgcc ggctactcac cgatcgccgc caaggacgag ctcggaccgg
    10741 acgaccgact gttcactccc catccattaa cccacgggtg gggactgcga accgggatcc
    10801 tgctgccgct gctggtgggc gcccgctcgg tggtgctgga cacgtgggag cccgaggcgg
    10861 cgctcgacct gctagtccgt accggagcca cggtcgtcct cgccgctccg ggcttcctgt
    10921 ccggcctggt cgcggaggcc aggaagcaat cggaaaggct cccggccgtc cgacggatcg
    10981 tcaccggcgc caccatcccg gacggactga tcaccgaact ggccgactgg ttcggcgtga
    11041 ccccgcagtc gacacggggc atgtccgaag tcatcggcgg cacccgaacc cggctcacgg
    11101 acccggtcga cctgccgccc cgcagccacc gagcaccgcg ggccgacctg gaaatcgtcc
    11161 ggagcgacgg gcagatgcgc gtcgacccgc ctgcggctct acgcgcacgg ctccagcttg
    11221 tgccgggcca ccgtgaaccg cgacacccgt gacagcgtcg tgccggccga gcacgaccag
    11281 gggtggtacg acaagacacc ggcgacctgg ccgtccccga cgcccatggc tacgcggatg
    11341 atctcccgga tcgccgcctc gccggccagg cttcttcgcc gtcggttgtc ggaatcgtca
    11401 gcggtcggtc ccaccagccg gatgccgtct cgggcgggcg tgcccgtcgc cgccaaaagc
    11461 tgaacctccg acttcacctg acaccgcaag cgtgaaacac cacgagaacg catcaaggag
    11521 tgaccgtgac ccaaaccgcg ccacaccagc ccgacgcggc cgaacagcag gcccgcgagc
    11581 aggcggtcac cgatgaggtg gtggccagct tcgccaccag cacctccgac cgcttccggg
    11641 aggtgatgac cagcctggtc cgacacctgc acgggttcgc ccgcgatgtg cggctgactc
    11701 acgccgagtg ggagaccgcg gtggacttcc tcacccgtac cgggcagatc tccaacggcc
    11761 ggcgtcagga gttcatcatg ctctccgacg tgctcgggct gtccatgctg accgtcggca
    11821 tcaacgcgcc ggccgcgcgg ggggcgaccg agtccaccgt gttcggcccg ttcttcgtcg
    11881 acgatgcccc ggaggtgccg ctcggcggtg acatcgccct gggtgccaag ggcaccccgt
    11941 gctgggtctc cgggcaggtg cgctccaccg acggcacgcc gctggccggg gtgcgcatcg
    12001 acgcctggga ggccgacgag gacggcctgt acgacaccca gtacgagggc aaccgcaccg
    12061 ccgggcgcgg ctggcaggtc accggggagc acggcgagta ccgcttttgg tcggtgctgc
    12121 ccacccacta ccccatcccc gacgacggac cggtgggcga gctgatcgcc gccgccggcc
    12181 gtgacccgat gcgcccggcc cacctgcact tcaaggtcac cgcccccggt caccgcacgc
    12241 tgatcaccca cgtgttcgtg gccggcgacg agtacctgca caacgacgcc gtgttcggcg
    12301 tcaaggacag cctcatcgcc gagttcaccg aacaccaggg ccgcaccgca ccaaacggca
    12361 ccactcgacg gtggacacaa gtgcggttcg acctggtact ggcccgggag gagcagccat
    12421 gaccgccccg accaccctga aggcaccaga atccgactac gacccgtata ccgacgccgc
    12481 gctgttgaat ccgtggcccg gctatcggga tctgcgggac gcggggccgg cggtttggtt
    12541 gcccaaatac gagatgttcg cgctcacccg gcacgcttcg gttggtgatg cgctgcggga
    12601 ctggagcacg ttcgcctccg cttacggggt gatgatgaac gatcccatga atcaggtact
    12661 gcgcggcaac acgctgtgca gcgacggcgc cgaccacgac gtgctgcgcc gggtggtgat
    12721 caaaccgctg cgcccgcaag cgatcaccga gctcaccgaa gagatcagcc gggaggccga
    12781 tgaactggtg gaacgggtgg tgaaacaggg aagtttcgac gcggcgacgg acttggcaca
    12841 tcacctgccg ttgacgatcg tgtcgaagct ggtcgggcta ccggaggagg gccgcgaacg
    12901 attgttggag tgggcctcag ctttgttcaa ctgtataggc ccgctcaacg agcgcgctat
    12961 ctcggcgttc cccatagtac aagagatggt cgattatgca accacccagg cggtacgcgg
    13021 caagctcaag ccgggcagtt ggggtgaagc gctgcacgac gcggccgacc gtggcgaggt
    13081 acccctagag gcggttccgg tgatgatggt cgactatatg ggtcccagct tggacaccac
    13141 gatcttcgca atcactagcg cggtgtggct gttcgcccag caccctgatc agtgggacct
    13201 ggtgcgcgag aacccgaagc tgatcccgag cgcggtgaac gaagtgctgc ggatggaggc
    13261 gccgatccag gacttctccc gctataccgt caaagaattc gacatggacg gagttacgat
    13321 cccgcccgat tctcgggcga tcgtgttcta cggtgcggcc aaccgcgacg agcgccagta
    13381 cccggacccc gaccgtttcg acgtgcgccg cagcccgacg aaccacatgg cgttcggtac
    13441 tggaccgcac gtatgcctgg gtctgtacct ggcccggctg gaaatgacgt ccctgttcac
    13501 cgcgctcgct cgccgagtgc gccgcttcga cctgggcgac accgaacgcg cgctgcacaa
    13561 catcctgcgc ggcttcacca aactacaggt cgccgtctca tagccagcgc tggcgcgtct
    13621 ctgacgcccc gccgcgcatt tcaccgacgc aagccccccg aggtcgcggc ccggctgcgc
    13681 accagcgccg gagttcacgg tctggaccag tgcctatctc aactcccaag ccggcacctg
    13741 cgggaagccc cctacaacat ggacgtgacg gtcgccgatg ccgcggtcaa gcagatcatc
    13801 gtcgcgcgcg tcgaacttct gtccgctgtc cccgccgtcg tggtgagcat cgtggtcaac
    13861 gccaggaatt ccggcgccgg agatcgtcgc cggtggcgag ggcccggggg tcccccgtgc
    13921 gggggacccg ccgatcccgc gccggatcat cgaaggtgtg gaattccgct accgcctcga
    13981 tccgcgatcg aaacacgaga ggcgaggtgc gcgacctgag cagttcattg gcgcgccagt
    14041 ccttgctcgt cgccgcggtg tgcctgctcg ctgacgtggc tggattcctc tccagaggcg
    14101 cgttgcgtcc ccaggactgg gcggtgctcg gcgatcgttg cggtcgacgc cgtgctcgcg
    14161 ctgcccgccc gctactcggg gtggtggtgt gacctgcggg tgagcgacac gccgaggatg
    14221 ctggtggagt agggcgggca cgacccgatg atcacgatgg ctctcgaggc cagatgatcg
    14281 tcggaagaaa gccgtgcccg cactcgcagc attatccggt caccatcgga gtcaggcgag
    14341 gcgagaccgg taattgaact ctgcgatcag atcatattcg acgacgacgg aaagtcagag
    14401 gctgcctcgc acgttggtgt tcgcgctgtc acggtcgttc gggaccgatt gtgccctccg
    14461 ccatcgccct gcgggagggt ttcggtgcat gccagaacgc catcctgtcc agcacgctgt
    14521 ctctgatcac gatggtgtgc aacagcacgc cggccaggtg cgccgcgaac gtcaggtaca
    14581 gcaggtaggc cgcggcgatg tgg
//
LOCUS       DASRSC010000050         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3945288,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000050 DASRSC010000000
VERSION     DASRSC010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3945288"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VFE39_02625"
     CDS             complement(<1..1203)
                     /locus_tag="VFE39_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6257523.1"
                     /translation="MEPEPLLDSLRNAVNAMPEDVPLRLHLATMLLRAGQRDEAVRHL
                     GAVLQRDPGNAQAMRMLTSPAEVPGPPASPPADVDTSAERGRGSSSPPTGPSPVQDVP
                     GGGAKSSGAGDASGAGDASGARAGGASGAGGPDGYDWTQAEDELRDVLPAMFVGEAGS
                     VSTGVDEARAYDAEHTGLTLADVAGLDEVKKRLEAAFLAPMRNPELRKLYGKSLRGGL
                     LLYGPPGCGKTFIARAVAGELGARFITVSFADLIDMFVGRSERNIHELFEVARRNAPC
                     VVFLDEVDAIGQKRSQLRNTPMRSAVNQLLLELDDVASDNTGVFLLAATNHPWDVDSA
                     LRRPGRFDRTLLVLPPDGPAREGVFRYHLRGRPVAGIDLAKLAKLTDGYSGADIAHIC
                     ETAAERALL"
     gene            complement(1293..>2088)
                     /locus_tag="VFE39_02630"
     CDS             complement(1293..>2088)
                     /locus_tag="VFE39_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET6257524.1"
                     /translation="EPGDTVAVHFVSMTPARDWAASTTVPLFGALTGTHLTALLHEPL
                     EELMWIWELDRDKGLCRFTAKKSDFTADLPMDPMHGTVGVAPAGGEVRSALVPDAFGG
                     NMDTPEMRAGTTCYLGVNVPGALLSLGDGHARQGEGETCGVAVECAMNTVLIVDLIKG
                     TGSGLPVTPWPRLESDAYVMSAGSARPLEDAFRIAQHDMVTWVAALCGLDPIDAYQFV
                     TQAVESPLANVCDTNYTSIAKMPKRYLPGQAYDGIHAHLRELAASL"
BASE COUNT          310 a          754 c          723 g          301 t
ORIGIN      
        1 gagcagcgcc cgctcggcgg cggtctcgca gatgtgcgcg atgtccgcgc ccgagtaccc
       61 gtcggtgagc ttagccagct tggccaggtc gatcccggcg accgggcggc cccgcaggtg
      121 gtagcggaac acgccctcgc gggcaggccc gtcgggcggc agcacgagca gcgtgcggtc
      181 gaagcgcccc gggcgccgca aagcggagtc aacgtcccag ggatgattgg tcgccgcgag
      241 caggaacacc ccggtattgt ccgacgccac gtcgtccagc tcgagcagca gctggttgac
      301 ggccgagcgc atcggcgtgt tccgcagctg cgaccgcttc tgcccgatgg cgtcgacctc
      361 gtcgaggaag accacgcagg gcgcgttgcg cctggcgacc tcgaagagct cgtgaatgtt
      421 gcgctcggag cggcccacga acatgtcgat caggtcggcg aacgacaccg tgatgaaccg
      481 cgcgccaagc tcgcctgcca ccgcccgggc gatgaacgtc ttgccgcacc cgggcgggcc
      541 gtacagcagc agcccgccgc gcagggactt gccgtagagc ttgcgcagct cgggattgcg
      601 catcggcgcg aggaacgcgg cctcaagccg cttcttgacc tcgtcgaggc cggccacgtc
      661 agccagcgtg aggccggtgt gctcggcgtc gtaggcgcgg gcctcgtcga ccccggtgga
      721 caccgacccc gcctcgccga cgaacatcgc gggaagcacg tcgcgcagct catcctcggc
      781 ctgcgtccag tcgtagccgt ccggcccgcc ggcgccactc gcaccgccgg ccctggcacc
      841 actggcatca cccgcaccac tggcatcacc ggcaccactg gacttggccc ctccgccagg
      901 cacgtcctgc accggagagg ggccggtggg gggagaggag gaaccacgcc cccgttcggc
      961 ggaagtgtcg acgtcggccg gcggtgacgc cggcggaccg gggacctcgg cgggagaggt
     1021 gagcattctc atggcctggg cgttccccgg atcgcgctgc aggacggcac cgaggtgccg
     1081 caccgcctcg tcgcgctgtc ccgcgcgcag cagcatcgtc gcgaggtgga ggcgcagcgg
     1141 cacgtcctct ggcatcgcgt tcaccgcgtt acgcaggctg tccagtagcg gttcaggttc
     1201 catgcgtccg ttccggggag ttgggagcga ccattccaca ctacgatccc cggtacgcaa
     1261 gtgggcaccg accggccaac aacgttttcc gcctaaagcg atgccgcgag ctccctgagg
     1321 tgggcgtgga tcccgtcgta tgcctgccct ggcaggtagc gcttgggcat cttggcgatc
     1381 gacgtgtagt tcgtgtcaca cacgttggcg agcggcgatt ccacggcctg cgtgacgaac
     1441 tggtaggcgt cgatcgggtc gagcccgcac agcgccgcca cccaggtcac catgtcgtgc
     1501 tgcgcgatgc ggaacgcgtc ttcgagcggc cgggcggacc cggcggacat cacgtacgcg
     1561 tcgctctcga ggcgcggcca cggagtcacc gggagccccg agccggtgcc cttgatcagg
     1621 tccacgatga gcaccgtgtt catcgcgcac tcgaccgcga ccccgcaggt ctcgccctcg
     1681 ccctggcgcg cgtgcccgtc gccgagcgac agcagcgccc ctggcacgtt gacgccgagg
     1741 tagcacgtgg tacccgcgcg catctccggc gtgtccatgt tcccgccgaa cgcgtcgggc
     1801 acgagcgcgg agcgcacctc gcccccggcc ggggccacgc cgaccgtgcc gtgcatcggg
     1861 tccatcggca ggtcggcggt gaagtccgac ttcttcgcgg tgaaccggca cagccccttg
     1921 tcgcggtcga gctcccagat ccacatgagc tcttccagcg gctcgtgcag caatgcggtc
     1981 aggtgcgtcc cggtcagcgc gccgaacagc ggcaccgtgg tcgacgccgc ccagtcccgc
     2041 gcgggggtca tggacacgaa gtgcaccgcc accgtgtcgc ccggctcg
//
LOCUS       DASRSC010000051        15694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3454532,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000051 DASRSC010000000
VERSION     DASRSC010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 15694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15694
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3454532"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1437)
                     /locus_tag="VFE39_02635"
     CDS             complement(<1..1437)
                     /locus_tag="VFE39_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HET6257525.1"
                     /translation="MIVAEEVGRALAELGVGHAFGVVGSGNFHVTNALRAGGVPFVAA
                     RHEGGAATMADAYARTSGRVGVLSVHQGCGLTNAMTGITEAAKSRTPMLVLAADTAAA
                     AVRSNFRIDQDALVEAVGAVAERVHSPVSALADTVRAYRTAVEQRRTVVLNLPLDVQA
                     AEVPEGRQVPDVPALAPVRPSATAVAELAALLAAAQRPVFVAGRGARGAREELLALAE
                     ACGALLATSAVANGLLHDDPWSLGISGGFASPLAAELITGADLVVGWGCTLNMWTMRH
                     GALIGPGARVVQVDLDADAIGANRPVDLGVLGAVAATAVDVLAVLGEHRRGYRTAEVR
                     AAIDDRGRWHTVAYDDLTGADEIDPRTLSIALDELLPTERTVAVDSGNFMGYPSAYLR
                     VPDEHGFCFTQGFQSIGLGLSTAIGAALARPDRLAVAALGDGGALMAAAELETVARLG
                     LPMVVVVYDDHGYGAEVHHFAGHDHATVV"
     gene            complement(1493..2266)
                     /locus_tag="VFE39_02640"
     CDS             complement(1493..2266)
                     /locus_tag="VFE39_02640"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET6257526.1"
                     /translation="MSVLQDLLDALRARTVEVVDLTAPLHSGTPILQLPPPFANASPF
                     GLTEISRYDERGPAWYWNDIQTSEHSGTHFDAPVHWVTGKDGEDIAQVPPARLLAPAA
                     VLDFSAESVKDPDFLLEVEHVRAWEAEHGPLPDGGWLLYRTGWDARSEDAGAFLNADD
                     TGAHTPGISVECAKWLAEEAPVLGLGVETVGTDAGGAHSFDPPFPCHSYLLGAGKYGL
                     TQLQNLARLPATGAVVIAGPLPIVGGSGSPARVLALVES"
     gene            complement(2519..3004)
                     /locus_tag="VFE39_02645"
     CDS             complement(2519..3004)
                     /locus_tag="VFE39_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257527.1"
                     /translation="MKLRLRNILRTAVVVPARPVWRGVLARIEQRVASSEQLQENRLQ
                     EATRQLKDESRAREADVTHLNQRTQDLQRDIDEFSDQLRRISAQLVAAEQRIADLERT
                     PVVVPGSDTEHHEARTLIHEVRTEHSRARAELAAVAFYEERIARLERAAEAGRRREEQ
                     R"
     gene            complement(3001..3492)
                     /locus_tag="VFE39_02650"
     CDS             complement(3001..3492)
                     /locus_tag="VFE39_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257528.1"
                     /translation="MTTFALDTRHGPARRGRRADIVVLHRAVVPGPGTFADHSSRLYL
                     LVEPGRLYWEREDRPDWWPVHPVSVPNAEFTVPLKALMGLAQEDASWPTTGTLVTYMM
                     TELFPDAVTRLAGTTIVEQSEQTSFTHDWGPAVKVTGEHRLVVESALLRSWVEQGRIE
                     LLR"
     gene            3742..4614
                     /locus_tag="VFE39_02655"
     CDS             3742..4614
                     /locus_tag="VFE39_02655"
                     /inference="COORDINATES: protein motif:HMM:NF015087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HET6257529.1"
                     /translation="MNAPQAPRVVAEIGCNHGGNLDLAKRMITVAATFCDVDAVKFQK
                     REPATLLAPEQYRAPHPEPRHAFGATYGEHREVLEFDIAQHAELVAHAGECGVTYSTS
                     VWDVPSAKAIIGLEPEFIKVPSATNLHTELLELLCSDFGGVIHLSLGMTTREEEQRIL
                     ELFRRHGRLGDLVLYACTSGYPVPFEDICLLEVQRLRDTYGGQVHAVGFSGHHLGIAA
                     DIAALALGAQYVERHFTLDRTSKGTDHAASLEPDGLRKLTRDLRNVAKALTLKPADVL
                     PIEEPQRAKLKWRP"
     gene            4611..5783
                     /locus_tag="VFE39_02660"
     CDS             4611..5783
                     /locus_tag="VFE39_02660"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HET6257530.1"
                     /translation="MTLAVVIPCRAGSKGIPGKNLALVAGRPLLDWTVEAALRATCVD
                     RVLVSTDDPAVRAHVRATWPGRVGLPVRSPATASDTATTESVLLDVLGTAEAVGITEV
                     GLVQATSPLLLAADLDGAHAMLRAGYDSVLSVVRQRRFRWGRGPDGASHADYDPAARP
                     RRQELAGYLVENGALYLTSREALVRTGVRISGRIGLYEMPEESYTEVDHPADLTVVDA
                     LLRRREAPPAPLVAAARIRLVLTDVDGVLTDNGMTYASDGTESKTYSARDGKGFELLR
                     LAGLRTAILTSEDGPHIRARATKLQVDAVYAGTQDKLADARELCARWGLTLDEVAFIG
                     DDVHDVALLTAVGLAGCPADAMPAAHGSAAYHCRALGGHGAFREFADMILAKRKFP"
     gene            complement(5951..6793)
                     /locus_tag="VFE39_02665"
     CDS             complement(5951..6793)
                     /locus_tag="VFE39_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6257531.1"
                     /translation="MTASRASGSGPPMRFDYGSHPSQFGELTLPADEAPVRGVVVVVH
                     GGFWRQAYGLELGRPLAADLARAGLAAWNIEYRRVPPDGAARTPSTTGDAGGWTATFD
                     DVAAALDLLAGPLQEAAGGRLPLGNVVAIGHSAGGHLATWLAARPGLQDRAPGAAPAV
                     LLRGAVSQAGVLDLVDAAERGVGNGAVERLLGGTPSAVPDRYALASPVARLPIGVPVV
                     CVHGTSDVNVPIRQSERFVAAAGNAAELITLPGVDHMAMIDVSSDAWRAGRDALERLL
                     PNQM"
     gene            complement(7079..7822)
                     /locus_tag="VFE39_02670"
     CDS             complement(7079..7822)
                     /locus_tag="VFE39_02670"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET6257532.1"
                     /translation="MSTAPLFLVDALPAGDRAVLDGPEGRHAATVKRLRAGEAVLLAD
                     GRGGLAHAEVDAAGRDTVALTIGDRSVLAPPAPRVLLAQALVKGDRGELAVEMATEAG
                     VDGILPWRASRCVVRWDDGPRGDKALARWRSTVREATKQARRPWLPVVEEPVTTEALA
                     RRVAGAAGALVLHEGATVPLAELAGELPAAGDLLLVVGPEGGVSDVEIEMLTAAGARP
                     VRLGHEVLRASTAGAVALGALGVLTGRWH"
     gene            complement(7854..9011)
                     /gene="dnaJ"
                     /locus_tag="VFE39_02675"
     CDS             complement(7854..9011)
                     /gene="dnaJ"
                     /locus_tag="VFE39_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET6257533.1"
                     /translation="MARDYYGILGVDTDAGPDEIKRAYRRLARELHPDVNPDAAAQER
                     FREVSAAYEVLSDPEKRRIVDLGGDPLGNGAGSGGGGGDPFSAFGFGDIMDAFFGGQG
                     GGRGRGPRSRVQPGADALIRMHLTLEECATGVMRDLTVDTAVLCAECTGSGCAPGSAP
                     TMCDICNGRGEVQSVQRSFLGQVVTSRPCPSCRGFGEVIPEPCRQCGGDGRVRSRRSV
                     GVRIPAGVADGMRVRLAGQGEVGPGGGAAGDLYVEVEEEPHELFTRDGADLHCTVQLP
                     MTAAALGTTLPLPTLDGTDELHVEPGTQAGTVRTLRGKGMPRLRSSGRVDGHGDLMVH
                     VDVVVPTKLDAKQTELLRQLAELRGEEQPDLAGGNRNGHGIFSRLRDSFGR"
     gene            complement(9193..10212)
                     /gene="hrcA"
                     /locus_tag="VFE39_02680"
     CDS             complement(9193..10212)
                     /gene="hrcA"
                     /locus_tag="VFE39_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET6257534.1"
                     /translation="MNADERRFAVLRAIVADYVSTHEPVGSKMIVERHNLGVSSATVR
                     NDMAVLEEDGLIAQPHTSAGRVPTDKGYRLFVDRLAQVKPLSAAERRALQAFLDGAVD
                     LDDVLRRSVRLLAQLTRQVAVVQYPTLTRSTVRHCEIVALTPARLMLVLITDSGRVDQ
                     RVVDLGEVLSDDQVAQLRALVNGSLAGRPLAAASVAVADLPDTVPSDLRDVVITLSTV
                     LIETLVEHPEERLLLGGTANLTRNAADFPGSLRQVLEALEEQVVVLKLLASAQDPGTV
                     TVRIGEENEAQEMRDTSVLSIGYGPGTVLGGMGVVGPTRMDYPGSIAAVHAVARYVGE
                     ILAGR"
     gene            10331..10936
                     /locus_tag="VFE39_02685"
     CDS             10331..10936
                     /locus_tag="VFE39_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257535.1"
                     /translation="MTAGEQPGWATLFDVLMRRTTLDAIAAGTVTVVFRRWDRPRVRP
                     GGTQRTSVGVLKFTAVEPVDVFDLTSEDASAAGFADLPALRQAQAGEGQLYRVRLRLA
                     GPDPRVELRSQATLSAEERTELARRLDRLDRASRSGPWTAAVLDLIAANPGTRAPDLA
                     GQLGRETLPFKRDVRKLKELGLTESLEVGYRLSPRGRAFRG"
     gene            11005..12111
                     /locus_tag="VFE39_02690"
     CDS             11005..12111
                     /locus_tag="VFE39_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257536.1"
                     /translation="MAAQRFSRRWLLVAAGAAGLVAAGAVPRPAAAVEVDAAALRGRI
                     LAGVPPHVGYAESTGRLGLPQIPQLESTGSLLGSTTRIRAFVAAADRWRVDELTPVGE
                     RDTYHLDGTEYLWDFGADQLTRVVGQTPLRLPRAADLLPPDLARRLLRLAPADPVAPL
                     PARRVAGRTAVGLRLTPSDPATTVGSVDIWADPATALPLRVEVAPRGGPPLLRTEWIE
                     VVDRSPEPAELTPTVPPGAGAVATSAADVSGALRSLDAPPPPDRLAGRERAPLSGTPD
                     AQLPGVGIYGTGLAAFALVPATGDLARRVVDGAAAAGGTAVQVPVGRAVRLATPLVSV
                     VVLASRRNGVLLVGTVAPELLEQAVRELPGRRRA"
     gene            12111..13058
                     /locus_tag="VFE39_02695"
     CDS             12111..13058
                     /locus_tag="VFE39_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6257537.1"
                     /translation="MADSPAAEIGNVISTAGLTKRYGRVTAVDDVDLQVPAGARFGLL
                     GPNGSGKTTLVRMLLGLVHPTSGSVELLGRAIPRHAATVLPRVGALVEGPAAWPHLSG
                     RANLRLLDAAGRRGRRRDRRARVEEALQRVGLGGVDRRPVRAYSLGMRQRLGIAAALL
                     RGPELLLLDEPTNGLDPRGIGEMRELLVALNEGGTTVVLSSHLLTEVEALCTRVGVMD
                     AGRLVLTEDLDALRVPTGRVLLRTPDPAAVVGLLDGQVVERNGQVVVVRDADPAALNA
                     RLVAAGIRVTELAAQRRTLEQVVLGLTGAGSDRVDGRGS"
     gene            13132..13962
                     /locus_tag="VFE39_02700"
     CDS             13132..13962
                     /locus_tag="VFE39_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6257538.1"
                     /translation="MIGVELRLMLRRRRVWLCWVLLCALPALVAVLLATTRLAPAPGQ
                     GPAFLSAVVSNGQLFPAAALALVLPVFLPITVAVVAGDAIAGEASTGMLRYLLVRPVG
                     RLRLLVAKLVSVAAFVLLAVLLVTLTSYVVGVAAFGFGPGASLGGAGGITSLSGAVIS
                     PTELAARTAAVVGYLALCMLALGAIGLFFSTLTDSPLAAALGVLAVVVTSAALLPLDA
                     AAAVRFYLPTTHWLAWIDLYRAPILWTAVRQGAEVQVSYVLVAFGAAWANFATKDITS
                     "
     gene            13986..14768
                     /locus_tag="VFE39_02705"
     CDS             13986..14768
                     /locus_tag="VFE39_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6257539.1"
                     /translation="MATLEFGEEGARQVERTYATPDVVAQRAQVLALLAPQAGERVLD
                     IGSGPGYLAESIADAVGPTGAVHGLDPSEAMNALAKARCTSRPWVHIQDGDAVALPFP
                     DGSFDAAVSTQVYEYVADLPRALAELRRVLRSGGRAVVLDTDWDSLVWHTEDRALHQR
                     VVAAWEEHLVHPRLPRRLAGLLRRAGFTVPEQRVVVLFNPQLHAETYSANTMELIAEF
                     VTGRQGLTAEDAAAWLADLRARADEGDYLFSLNRYVFLATAA"
     gene            complement(14734..15198)
                     /locus_tag="VFE39_02710"
     CDS             complement(14734..15198)
                     /locus_tag="VFE39_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257540.1"
                     /translation="MDGWESPTSGSIPPGPVTHARGRTVVLPRAGGTHRFGPIALLTA
                     SLQGWPWLRRTYPAVPGWSGRAYLALGVLPGGVAVLAVAPFGVPGGLTGRVANTMLAV
                     LWLGTALAGYRAARARQACPPRGARRGPATGARHLVRSGREVRRRLRGTRTG"
     gene            complement(15222..>15694)
                     /locus_tag="VFE39_02715"
     CDS             complement(15222..>15694)
                     /locus_tag="VFE39_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HET6257541.1"
                     /translation="VDVVGYSAGGVVAGLWVARDDGALKARRVVTLGAPLAGTTLAAA
                     ASASAPDACPAACRQLAPGSAELAELARARVGTALPWLSIWTTDDETVTPPDSARLAG
                     AVNVPVQSVCPGARVSHSGLPTDAAVTGLVLEAISRAALVAGGDCTTLRAAGAG"
BASE COUNT         2139 a         5823 c         5588 g         2144 t
ORIGIN      
        1 caccacggtg gcatggtcat ggcccgcaaa atggtgcacc tcggcgccgt agccgtggtc
       61 gtcgtagacc accacgacca tcggtagccc gagccgcgcc acggtctcca gctccgcggc
      121 cgccatcagc gcaccgccgt cgcccagcgc tgcgacggcg agccggtcgg ggcgggcgag
      181 cgcggccccg atcgccgtgg acagccccaa cccgatcgac tggaacccct gcgtgaaaca
      241 gaacccgtgc tcgtcgggca cccggagata ggcactcgga tagcccatga agttgccgga
      301 gtcgacggcc accgtccgct ctgtgggcag cagctcgtcc agcgcgatcg acagcgtcct
      361 ggggtcgatt tcgtccgccc cggtgagatc gtcgtaggcg acggtgtgcc agcgaccgcg
      421 gtcgtcgatc gccgccctca cctcggcggt gcggtagccg cggcggtgct cgccgagcac
      481 tgccagtacg tcgaccgccg tcgcggccac cgcacccaac accccgaggt ccaccggccg
      541 gttcgcacct atcgcgtcgg cgtccaggtc cacctgcacc acccgggcgc ccggcccgat
      601 cagggcacca tggcgcatgg tccacatgtt cagcgtgcag ccccagccga ccacgaggtc
      661 ggcccccgtg atcagctcgg cggccagcgg cgaggcgaac ccacccgaga tgcccagcga
      721 ccaggggtcg tcgtggagca gcccgttcgc gacggccgac gtggccagca gcgcaccgca
      781 agcctcggcc agcgccagca gctcctcccg cgctccccgt gcaccccggc ccgccacgaa
      841 caccgggcgc tgcgccgccg cgagcagtgc ggcgagctcc gccaccgccg tcgcggacgg
      901 ccgcaccggg gccagcgcgg gcacgtccgg cacctgccgg ccctcgggca cctcggccgc
      961 ctgcacgtcg agcggcaggt tgagcaccac ggtgcgccgc tgctcgacgg cggtgcggta
     1021 ggcccggacg gtgtcggcaa gcgccgacac gggcgagtgc acgcgctccg ccaccgcgcc
     1081 gaccgcctcg accagcgcgt cctgatcgat gcgaaagttc gaccgcacgg cggcggcagc
     1141 ggtgtcggcc gccagcacga gcatcggggt acggctcttc gccgcctccg tgatcccggt
     1201 catcgcgttg gtgagcccgc agccctggtg caccgacagc acgccgaccc gcccgctcgt
     1261 ccgcgcgtag gcgtcggcca tcgtcgcggc tccgccctcg tgccgcgccg cgacgaacgg
     1321 gacgccgccc gcgcgcagcg cgttggtcac gtggaagttg ccgctgccca ccaccccgaa
     1381 cgcgtgcccg actccgagtt cggcgagcgc gcggccgacc tcctccgcga cgatcaccgg
     1441 cctgcctcac acctgctcgg cggcaccgcg cggcccggcg cgatctcctg catcacgact
     1501 cgaccaacgc cagtacccgc gccggactgc ccgagccgcc gacgatcggc aacggcccgg
     1561 cgatcaccac cgcgccggtg gcggggagac gggccaggtt ctgcagctgg gtcagcccgt
     1621 acttgcccgc cccgagcagg taggaatggc acgggaacgg cgggtcgaac gagtgcgcgc
     1681 cgcccgcgtc cgtacccacc gtctcgacgc cgaggccgag caccggcgcc tcctcggcga
     1741 gccacttcgc gcactcgacc gagatgccgg gcgtgtgcgc accggtgtcg tcggcgttca
     1801 ggaaggcccc ggcgtcctcg ctgcgggcat cccaacccgt ccggtagagc agccaaccgc
     1861 catccgggag cggaccgtgc tcggcttccc aggcacggac gtgctcgacc tcgagcagga
     1921 agtcggggtc tttcacgctc tcggcggaga agtccagcac cgctgccggg gccagcagcc
     1981 gcgcgggcgg cacctgcgcg atgtcctcgc cgtccttccc ggtcacccag tgcaccgggg
     2041 catcgaagtg cgtgccggag tgctcggagg tctggatgtc gttccagtac caggcggggc
     2101 cgcgctcgtc gtagcggctg atctcggtga gcccgaacgg cgacgcgttg gcgaacggtg
     2161 gcggcaactg caggatcggg gtgccgctgt gcagcggagc ggtgaggtca acgacctcca
     2221 cggtgcgggc gcgcaacgcg tcgagcaggt cctgcagcac cgacatggcg ggctcctcgg
     2281 ggttcggcgg ggttcttcga agaccgacca atcgtgtcgc aacgggtcgg ctccgcccag
     2341 cgcgcgcacc ggacgaaccg ttcgtcggtg ccggagccgc ccggagcacc gccgcgatcg
     2401 gcagcagcag gaacatcggc cagccatccc aaccgaaacg gcgatgcaag cctcaacgca
     2461 ctagtcgctg gttgtcgtac gccggtggcg ttttgctggc cgaggtcgct cggcttcctc
     2521 agcgctgctc ttcccgacgt cgcccagcct cagcggcccg ctcaagccgc gcgatacgtt
     2581 cctcgtagaa ggcgacggca gcgagttcgg ctcgagctct gctgtgttct gtgcgcacct
     2641 cgtgaatcag tgtgcgcgcc tcatgatgtt cggtgtccga gccggggact acgacaggag
     2701 tgcgttcaag gtctgctatg cgttgctcgg ccgccacgag ctgggcactg atccgacgca
     2761 gctggtcgct gaattcatct atgtcgcgtt ggaggtcctg agttcgctgg ttcaagtgcg
     2821 tcacgtccgc ttcacgcgcg cgtgactcgt ccttgagctg tcgagtggcc tcctgaaggc
     2881 ggttctcctg cagctgctct gagctcgcca ctctctgctc gatgcgagcc agtacgcctc
     2941 gccagacggg ccgagctggg accacgacgg ctgtccgtaa aatgttgcgc aggcgcagtt
     3001 tcatcggagc agctcaatcc ggccctgttc gacccaggac cgtagcagag ccgactcgac
     3061 gacgagccga tgttctccgg tgaccttcac tgctggcccc cagtcgtgcg tgaagctcgt
     3121 ctgctcggac tgttcgacga tggttgtccc ggccaatcga gtcaccgcgt cggggaacag
     3181 ttccgtcatc atgtaggtca ccagggtgcc agtagtgggc caggaagcgt cttcttgggc
     3241 caggcccatc aaagctttga gcggaacggt gaactcggcg ttaggcaccg aaaccgggtg
     3301 taccggccac cagtcgggtc ggtcctcccg ttcccagtac agccgcccgg gctcgacgag
     3361 cagatagagc cgagatgaat gatcggcgaa cgttcctggc cccggcacga cggcgcggtg
     3421 cagaaccacg atgtcggcgc gccgaccacg acgggcaggg ccgtgtcgcg tgtcgagcgc
     3481 gaatgtggtc atccgtacga caagatcgct gtcgtcgatc gtcgcagccc gctcggcgct
     3541 gtcgctgagc gggccgttcc cgacgacggt gacggtacgg ggcgggacgg accgggcata
     3601 agtgcacaaa agcctcgcga gcacccggcg ctgctcggac tgcgcaggca ccgtgatcat
     3661 cacgcgcatc aaactaaacg acgatggagc gcttcctggc cccgggtgtg ggtggcggga
     3721 gcagcccgtc agaatcggcg cgtgaatgct ccccaagcgc cacgcgtcgt cgccgagatc
     3781 ggctgcaacc acggtggcaa cctggacctc gccaagcgca tgatcacggt ggccgccacg
     3841 ttctgcgatg tcgacgcggt gaagttccag aagcgcgaac cggcgacgct gctcgcgccc
     3901 gagcagtacc gcgcgccgca cccggagccg cgccacgcgt tcggcgcgac ctacggcgag
     3961 caccgcgaag tcctggagtt cgacatcgcc cagcacgccg agctggtggc gcacgcgggc
     4021 gagtgcggcg tcacctacag cacgagcgtg tgggatgtgc cctcggcgaa ggccatcatc
     4081 gggttggagc cggagttcat caaggtcccg tcggccacga acctgcacac cgagctgctg
     4141 gagctcctct gctcggactt cggcggcgtc atccacctgt cactgggcat gacgacgcgc
     4201 gaggaggagc agcggatcct cgagctgttc cgccgccacg gccgcctcgg cgacctggtg
     4261 ctctacgcgt gcacctcggg ctacccggtg ccgttcgagg acatctgcct gctggaggtg
     4321 cagcgactgc gcgacaccta cggcgggcag gtgcacgccg tcgggttctc cggccaccac
     4381 cttggcatcg cggcggacat cgccgcgctc gcgctcggcg cgcagtacgt ggagcggcac
     4441 ttcacgctcg accgcaccag caagggcacc gaccacgccg cgtcgctcga acccgacggg
     4501 ctgcgcaagc tcacccgcga cctgcgcaac gtcgccaagg cgctcaccct caagcctgcg
     4561 gacgtgctgc cgatcgagga gccacagcgc gccaagttga agtggcgccc gtgaccctcg
     4621 ccgtcgtgat cccgtgccgc gcgggcagca agggaatacc cggcaagaac ctggcgctgg
     4681 tcgccggccg gccgctactt gattggacgg tcgaggccgc cctccgcgcg acgtgcgtcg
     4741 accgggtgct cgtatccacc gacgacccgg cagtgcgcgc ccacgttcgg gccacatggc
     4801 cggggcgcgt cgggctaccc gtccgctccc ccgccacagc atcagacacc gccaccaccg
     4861 agagcgtcct gctcgacgtg ctcggcaccg ccgaggcggt cgggatcacc gaggtcgggc
     4921 tggtgcaggc cacgagcccg ctgctgctcg cggccgatct cgacggggca cacgccatgc
     4981 tccgcgcggg ttacgacagc gtgctgtcgg tggtgcggca gcgccgcttc cgctggggcc
     5041 gcgggcccga cggggcgagt cacgcggact acgaccccgc cgcccggccc cgccgccaag
     5101 agctcgccgg ctacctggtc gagaacggtg cgctctacct aaccagccgg gaagcgctcg
     5161 tccgcaccgg cgtgcggatc agcgggcgca tcgggctcta cgagatgccc gaggagagct
     5221 acaccgaggt ggaccatcca gccgacctca ccgtcgtcga cgccctgctg cgccgccgcg
     5281 aagccccgcc ggccccgctc gtcgccgccg cccggatccg gctcgtgctc accgacgtcg
     5341 acggcgtcct gaccgacaac ggcatgacct acgccagcga cggcaccgag tccaagacct
     5401 acagcgcgcg cgacggcaag ggcttcgagc tgctgcggct cgccggcctg cgcaccgcga
     5461 tcctgaccag cgaggacggc ccacatatcc gcgcacgagc aacgaagcta caagtcgatg
     5521 cggtctacgc aggcacacag gacaagctcg ccgacgcccg cgagctgtgc gcgcgctggg
     5581 gactcactct ggacgaggtg gcattcatcg gcgacgacgt acacgacgtc gcactgctga
     5641 ctgctgtcgg cttggccgga tgcccagccg atgccatgcc cgccgcacat ggcagcgctg
     5701 cctaccactg ccgagcactt ggcggacacg gcgctttccg cgagttcgcc gacatgatac
     5761 ttgccaaacg caagtttcct tgacctgaca gatctcgcgc ccgctgatcc gcagtggtcg
     5821 ccgaacctca ccaggatgtt ctacgcacct ctcggaacgt gtgtcggtac tgcgcggtga
     5881 tggggcggcg ctgtgcactg atctcctgga gcagtcacgc ggccactgag gtcgacaacg
     5941 tcacgggtca ctacatctgg tttggcaaca ggcgctccag tgcgtcgcgg ccggcgcgcc
     6001 acgcgtcgct cgacacgtcg atcatcgcca tgtggtccac gcccggcagc gtgatcagct
     6061 ccgccgcgtt tcccgccgcc gccacgaagc gctcgctctg ccggatcgga acattgacgt
     6121 ccgacgtgcc gtgcacgcac accacaggca cgccgatcgg gaggcgggcg accggtgagg
     6181 cgagcgcgta ccggtccgga acggcggagg gcgtcccacc gagcaggcgc tccacggcgc
     6241 cgttgccgac cccgcgctca gcggcgtcga cgaggtccag cacgccggcc tggctgacgg
     6301 ctccgcgcag cagcacggca ggggccgcgc cgggcgcgcg atcctggagt ccgggacggg
     6361 cggcgagcca ggtggccagg tgaccgccgg cggagtgccc gatggccacg acattgccca
     6421 gtgggagccg accgcctgct gcttcctgca gcggaccggc gagcaggtcc agcgcggctg
     6481 cgacgtcgtc gaaagtagcc gtccagccgc ccgcgtcccc ggtggtggac ggtgtgcggg
     6541 cagcaccgtc cggaggcacg cgccggtact cgatgttcca cgccgcgagc ccggcgcgtg
     6601 cgaggtcggc ggccagcggc cggcccagct ccagcccgta cgcctgccgc cagaacccac
     6661 cgtgcacgac gacgaccacc ccgcgcacgg gcgcttcgtc cgccggcagg gtcagctcac
     6721 cgaactgact cgggtggctg ccgtagtcga accgcattgg tggcccggag cctgatgcgc
     6781 gagaagcagt cacggcgcca ggttgccacg ccgcggtgga cgtgggggga cacccaccgc
     6841 ggcgtggctc cgccggctga gggggactcc ccggcggccc cgtgggtgcg aacacccgcg
     6901 gcctggccgg tcatctgcag cgaccggagc gtcagacgat agcccggccc accgcggacg
     6961 ggctgccacc tcacccggat gacgagggag tccggccgct cgggcgacaa ctccatctac
     7021 ggccggtacc ccggatgggc cgtgcgggcc gccagtagcg agcccgacgc tggtggcgtc
     7081 agtgccagcg accggtcagc acgccgagag ccccgagggc gacggcgccc gccgtcgagg
     7141 cgcgcagcac ctcatggccg agccgcaccg gccgcgctcc cgcggctgtg agcatctcga
     7201 tctcgacgtc gctgacgccg ccttccggac cgacgacgag caggaggtcc ccggcggccg
     7261 gcagctcgcc cgcgagctcc gccagtggca ccgtggcccc ctcgtggagc accagtgcac
     7321 ccgcggcgcc ggccacccgc cgtgccagtg cctccgtcgt cacaggctcc tcgacgaccg
     7381 gcagccacgg gcgacgggcc tgtttggtcg cctcgcggac cgtgctgcgc cagcgggcca
     7441 gcgccttgtc cccgcgcggc ccgtcgtccc accgcaccac gcaccgcgac gcacgccacg
     7501 gcaggatccc gtccaccccg gcctcggtgg ccatctcgac ggcgagctcg ccgcggtcgc
     7561 ccttcaccag cgcctgggcg agcagcacgc gcggggcagg cggagcgagg acgctgcgat
     7621 caccgatcgt cagcgcgacg gtgtcgcggc cggccgcgtc gacctcggcg tgcgccagcc
     7681 cgcctcggcc gtcggccagc agcacggcct ccccggcgcg cagccgcttc acggtcgccg
     7741 cgtgccgccc ctcgggaccg tccagcaccg cgcggtcccc cgccggcagc gcgtcgacga
     7801 ggaacagggg cgcggtgctc acgagtcaat cccccgggga gtgcgcccgg cgctcagcgg
     7861 ccgaacgagt cgcgcaggcg ggagaagatg ccgtgcccgt tgcggttgcc gcctgcgagg
     7921 tcgggctgct cctccccacg cagctcggcg agctggcgca gcagctcggt ctgcttggcg
     7981 tcgagcttgg tcggcaccac gacgtcgacg tgcaccatga ggtcaccgtg cccgtccacc
     8041 cggccactgg atcgcagccg cggcatcccc ttgccacgca gggtgcgcac cgtgccggcc
     8101 tgcgtgcccg gctcgacgtg cagctcatcg gtgccgtcga gggtgggcaa cggcagggtc
     8161 gtgccgaggg cggccgcggt catcggcagc tgcacggtgc agtggaggtc ggcgccgtca
     8221 cgcgtgaaca gctcgtgcgg ctcctcctcg acctccacgt acaggtcgcc cgccgcgccg
     8281 ccaccgggcc cgacctcacc ttgcccggcc agccggacgc gcatgccgtc ggccacgccc
     8341 gccgggatcc gcaccccgac cgaacggcgc gagcggaccc ggccgtcgcc cccgcactgc
     8401 cggcagggct cggggatgac ctcaccgaaa ccgcggcagc tggggcacgg ccgggacgtg
     8461 acaacctggc cgaggaacga gcgctgcacg ctctgcacct caccgcggcc gttgcagatg
     8521 tcacacatcg tgggtgcact gccgggcgcg cagcccgagc cggtgcattc cgcacagagc
     8581 acggcggtgt cgacggtgag gtcacgcatg accccggtgg cgcactcctc cagtgtgagg
     8641 tgcatgcgga tcagcgcgtc ggcgccgggc tgcacgcggc tgcgcggacc gcgcccgcga
     8701 ccgccgccct gcccgccgaa gaacgcgtcc atgatgtcgc cgaagccgaa ggcgctgaac
     8761 gggtcgccgc cgcccccacc ggagccggcg ccgttgccca gcgggtcgcc gccgaggtcg
     8821 acgatccggc gcttctccgg gtcggacagc acctcgtaag ccgcgctcac ctcgcggaac
     8881 cgctcctgcg ccgccgcgtc ggggttgacg tcggggtgca gctcgcgcgc gagcctccgg
     8941 taggcccgct tgatctcgtc gggcccagcg tcggtgtcga cgcccaggat cccgtagtag
     9001 tcccgtgcca ccctggcgtt ccccatggtc cttctcgctc agccgttcat caactcggtc
     9061 gtgcccggat ccgaggttgc ccgggtccga cagtgtcagt caggtcgtgc ccactcaggt
     9121 cgtgcccact caggtcgtgc aacgccggtc gagccggcac atcatcgtca attctgcgct
     9181 ccgcgagctc cgctagcgcc ccgccaggat ctcacccacg taccgggcga ccgcatggac
     9241 cgccgcgatc gagcccgggt agtccatccg ggtgggcccg acgactccca tcccgcccag
     9301 tacggtgcca ggcccgtaac cgatcgacag tacggacgtg tcgcgcatct cctgggcctc
     9361 gttctcctcc ccgatccgca cggtgacggt gcccgggtcc tgcgcggagg cgagcagctt
     9421 gaggaccacc acctgctcct cgagcgcttc gaggacctgc cgcagcgacc ccgggaagtc
     9481 ggcggcgttg cgggtgaggt tggcggtgcc accgagcagc aggcgctcct cggggtgctc
     9541 gacaagggtc tcgatgagca cggtggagag tgtgatcacc acgtcacgca ggtcggaggg
     9601 cacggtgtcg ggcaggtcgg ccaccgccac ggacgcggcc gcgagaggcc ggcccgcgag
     9661 cgacccgttg accagggcgc ggagctgggc gacctggtcg tcggagagca cctcgccgag
     9721 gtccacgacc cgctggtcca cgcgtccgct gtcggtgatg agcaccagca tcaggcgcgc
     9781 cggggtgagt gccacgatct cgcagtggcg caccgtggag cgggtgagcg tcgggtactg
     9841 gacgacggcg acctggcggg tgagctgcgc cagcagccgg acgctgcgcc gcaggacgtc
     9901 gtcgaggtcg acggcgccgt cgaggaacgc ctgcagtgcc cgccgctcgg ccgctgagag
     9961 cggcttgacc tgcgccaatc ggtcgacgaa caggcggtag cccttgtcgg tgggcacccg
    10021 gcccgcgctg gtgtggggct gggcgatgag cccgtcctcc tccagcacgg ccatgtcgtt
    10081 gcggaccgtg gcgctcgaca cgcccaggtt gtgccgctcg acgatcatct tcgagccgac
    10141 cggctcgtgg gtcgacacat agtcggcgac gatcgcccgc aggaccgcga agcgacgctc
    10201 gtcggcgttc acgcggcgct cctccccgat tcgtggtgtt ccgcccagtc taggtgttct
    10261 ccgcgcggag gggagcggtc gtcgtgtctg catgatcatg gtggatgagc gtcgcctggt
    10321 caggtggtcg gtgacggctg gggagcagcc cggctgggcc acactgttcg acgtgctgat
    10381 gcggcgcacg accctggacg cgatcgcggc tggaaccgtc acggtcgtgt tccgccgctg
    10441 ggaccgcccc cgggtgcgac cgggcgggac gcagcgcacg tccgtcgggg tgctgaagtt
    10501 taccgcggtc gagccggtcg acgtgttcga cctcacctcg gaggacgcca gcgccgcggg
    10561 cttcgccgac cttccggcac tgcggcaggc ccaagcgggc gaggggcagc tctatcgggt
    10621 ccggttgcgg ctggccggtc cggacccacg ggtggagctg cgttcgcagg ccacgctgag
    10681 cgccgaggag cgcacggagc tggcccgtcg gctggaccgc ctggaccggg cgtcgcggag
    10741 cgggccgtgg accgcagcgg tgctggacct gatcgccgcg aaccccggca cccgggcgcc
    10801 ggacctcgcc gggcagcttg gccgggagac gctgccgttc aagcgcgacg tgcgcaagct
    10861 caaggagctc ggcctcaccg agagtctcga ggtcggctac cggctctccc cccgtggacg
    10921 ggccttccgc ggctgacgcc tccgggggcg cctacccggg gcgcccaccc ggggttcaca
    10981 accacagtgg cagggtggca cgacgtggcc gcgcaacgct tcagccggcg ctggctcctg
    11041 gtcgcggccg gcgccgcggg tctggtcgcc gcgggcgctg tgccgcggcc cgccgccgcg
    11101 gtggaggtcg acgccgccgc gctgcgcggt cggatcctcg ccggggtgcc gccgcacgtc
    11161 ggctacgccg agagcacggg ccggctcggg ctgccgcaga tcccgcagct ggagtcgacc
    11221 ggctcgctgc tcggttccac cacccgcatt cgcgcgttcg tcgcagcggc ggaccgctgg
    11281 cgcgtcgacg agctgacgcc ggtcggggag cgtgacacct accacctcga cgggaccgag
    11341 tacctctggg acttcggggc ggatcagctc acgcgagtgg tcggacagac accgttgcgg
    11401 cttccccgtg cggccgacct gctcccgccc gacctcgcgc gccggctgct gcggctcgcc
    11461 ccggccgatc ccgtggcccc gctgccggcc cggcgcgtcg cgggccggac cgccgtcggg
    11521 ctgcggctca cgccgtccga cccggccacc accgtcggca gcgtggacat ctgggccgac
    11581 ccggcaaccg cgctgccgct gcgggtcgag gtcgcgccgc gcggcgggcc tccgctgctt
    11641 cgcaccgagt ggatcgaggt cgtggaccgc agcccagaac ccgcggagct gacgcccacc
    11701 gttccgccgg gcgcgggagc ggtggcgacc tcggcggccg acgtcagcgg tgccctgcgc
    11761 agcctcgacg ccccgccgcc tcccgatcgt ctcgcgggcc gtgaacgggc gccgctctcg
    11821 ggcaccccgg acgcccagct gccgggcgtc gggatctacg ggacgggcct ggcggcgttc
    11881 gcgctggtcc cggcgaccgg tgacctcgcc cgtcgcgtag tggacggcgc ggccgcggcc
    11941 ggcggcaccg cagtgcaggt gccggtcggc cgggcggtcc ggctggccac gccactggtg
    12001 tcggtcgtcg tcctggccag caggcgcaac ggggtgctgc tcgtcggaac ggtcgctccc
    12061 gagctgctgg agcaggccgt gcgggagctc cccggccgga ggcgcgcctg atggccgatt
    12121 cccccgccgc cgagatcggc aacgtgatca gcacagccgg gctcaccaag cggtacggcc
    12181 gcgtgaccgc agtcgatgac gtcgacctgc aggtgcccgc gggcgcgcgc ttcggtctgc
    12241 tcggacccaa cggctcgggc aagaccacgc tggtgcgcat gttgctcggc ctggtgcacc
    12301 cgacgtcggg ctcggttgag ctgctggggc gcgcgatacc ccgccacgcc gccaccgtgc
    12361 tcccccgcgt cggcgcactc gtcgagggtc ccgccgcatg gccgcacctc tccggacgcg
    12421 ccaacctgcg gctcctggac gcagccggac ggcgaggcag gcgccgggac cgccgcgccc
    12481 gcgtggagga ggcgctgcag cgcgtcgggc tcggtggcgt cgaccgccgc ccggtgcgcg
    12541 cctactccct cggcatgcgg cagcgcctcg gcatcgccgc agcgctgctg cgcgggccgg
    12601 agctgctgct gctcgacgag ccgacgaacg gcctcgaccc gcgtggcatc ggcgagatgc
    12661 gggagctgct ggtggcgctc aacgagggtg gtacgacggt cgtgctgtcc agccacctcc
    12721 tcaccgaggt cgaggcgctg tgcacccgcg tcggcgtgat ggacgcggga cggctcgtcc
    12781 tgaccgagga cctcgacgcg ctgcgcgtcc cgaccggccg ggtactgctg cgcaccccgg
    12841 acccggctgc ggtcgtcggg ttgctcgacg ggcaggtggt ggagcgcaat ggccaggtcg
    12901 tggtcgtgcg cgacgccgat ccagccgcgc tgaacgcccg gctggtggct gccgggatcc
    12961 gcgtcaccga gctggccgcg cagcgccgga cactggagca ggtggtgctg gggctgacgg
    13021 gagccggctc ggaccgggtg gacgggaggg gttcgtgatc gagcctgcga gatcaccatt
    13081 cagcacagca tcggccggca cggcgacgac gagcgcccgc gaggagtcga cgtgatcggc
    13141 gtcgagctgc ggctgatgct gcgccgccgc cgggtgtggc tgtgctgggt gctgctctgc
    13201 gcgctgcccg cgctcgtcgc ggtgcttctt gcgaccaccc ggctggcccc agcgcccggg
    13261 cagggcccgg cattcctgtc cgccgtcgtc agcaacgggc agctgttccc ggcggccgca
    13321 ctggcgctgg tgctgccggt gttcctgccg atcaccgtgg ccgtcgtggc cggggacgcg
    13381 atcgccggcg aggcgagcac cgggatgctg cgctacctgc tggtccgccc ggtgggacgg
    13441 ctgcggctgc tggtggccaa gctcgtgtcg gtggcggcgt tcgtgctcct cgccgtgctg
    13501 ctggtgacgc tcaccagcta cgtcgtcggg gtggccgcgt tcggcttcgg gccaggagcc
    13561 tcgctcggcg gggccggggg gatcacgtcg ctgtccggag cggtgatcag tccgaccgag
    13621 ctggccgcgc gcaccgcggc agtcgtcggg tatctcgcgc tctgcatgct cgcgctgggc
    13681 gcgatcgggc tgttcttctc cacgctcacc gactccccgc tggccgcggc gctcggggtg
    13741 ctcgccgtcg tggtcacgag tgcggcgctg ctgccgctgg acgcggccgc cgccgtccgg
    13801 ttctaccttc cgacgacgca ctggctggcc tggatcgacc tctaccgcgc ccccatcctc
    13861 tggactgctg tccggcaggg tgcggaggtc caggtgtcgt acgtgcttgt cgcgttcggg
    13921 gcagcgtggg ccaacttcgc cacgaaggac atcacgagct gagcgggcgg gcctaacctg
    13981 cagggatggc gacgttggag ttcggcgagg agggcgcccg ccaggtcgag cgcacctacg
    14041 cgacccccga cgtcgtcgcg cagcgggcgc aggtgctggc gctgctcgcg ccgcaggcgg
    14101 gtgaacgtgt gctcgacatc gggtccggtc cgggctacct ggccgagtcg atcgccgacg
    14161 ccgtgggccc gacgggcgcg gtgcacgggc tcgatccgag cgaggcgatg aatgcgctcg
    14221 ccaaggcccg ctgcacgagc cggccctggg tccacatcca ggacggggac gcggtcgccc
    14281 tgcccttccc cgacggctcg ttcgacgccg ccgtgtcgac gcaggtgtac gagtacgtgg
    14341 ccgatctgcc gcgcgcgctc gccgagctgc ggcgggtgct gcggtcaggt gggcgggcgg
    14401 tggtcctgga caccgactgg gactcgctgg tgtggcacac cgaagaccgg gcgctgcacc
    14461 agcgggtcgt cgcggcgtgg gaggagcacc tcgtccaccc ccgcctgccg cggaggctgg
    14521 ccggcctgct gcgccgcgcc ggcttcacgg tgcccgagca gcgagtggtg gtgctgttca
    14581 acccgcagct gcacgccgag acgtacagcg cgaacacgat ggagctgatc gcggagttcg
    14641 tcacgggccg gcagggcctc accgccgagg acgccgcggc ctggctggcc gacctgcgcg
    14701 ctcgagccga cgagggcgac tacctgttca gcctcaaccg gtacgtgttc ctcgcaaccg
    14761 ccgcctgacc tcacggcctg acctcacgag gtgacgggca ccggtcgccg gtccgcgtcg
    14821 cgcaccgcgg ggcgggcagg cctgccgggc ccgtgcggcc cgatagccgg ccagcgccgt
    14881 gcccagccac aggaccgcga gcatcgtgtt cgcgacgcgc ccggtgaggc cgcccggcac
    14941 gccgaagggc gcgacggcca gcacggccac cccaccgggc aatacgccga gggcgaggta
    15001 ggcccggccg ctccagccgg gcacggcggg gtaggtacgc cgcagccacg gccagccctg
    15061 cagcgacgcc gtcagcagcg cgatcgggcc gaaccgatgt gtgccaccag cgcggggtag
    15121 aacgacggtg cgtcctcggg catgggtaac cggtccgggc gggatcgagc ccgaggtagg
    15181 gctttcccac ccatccaccc acccgtccgc ggcgggaccc ctcagcccgc tcccgcggcc
    15241 cgcagggtcg tgcagtcccc gccggcgacg agggcggccc gcgagatcgc ctccagcacc
    15301 agccccgtga cggccgcgtc ggtcggcagc ccggagtggc tcacgcgcgc gccgggacag
    15361 accgactgca cgggaacgtt cactgcaccc gccagccggg ccgagtccgg cggggtgacg
    15421 gtctcgtcgt cggtggtcca gatcgagagc cacggcaacg ctgttccgac cctcgccctg
    15481 gccagctcgg ccagctccgc gctgcctgga gccagctgcc ggcacgcggc cgggcaggcg
    15541 tccggcgcgc tcgccgacgc cgccgccgcg agcgtcgtgc cggcgagtgg cgcacccagg
    15601 gtcaccaccc ggcgggcctt caaggcgccg tcgtcgcggg cgacccagag cccggccacc
    15661 accccgcccg ccgagtagcc gaccacgtcc accg
//
LOCUS       DASRSC010000052         4643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4929798,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000052 DASRSC010000000
VERSION     DASRSC010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4929798"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1259)
                     /locus_tag="VFE39_02720"
     CDS             complement(165..1259)
                     /locus_tag="VFE39_02720"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF010041.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HET6257542.1"
                     /translation="MAVRTVGVEEEFLLVDPATGRPRAVGHAVLATSAAEDAEDDLAA
                     ELHREQLETGTKPCHTLDELSAELRSARATAAAAAGDVGVALAPLATSPLAVEPTVSP
                     SRRYEEMARRFGRTVSEQLTCGCHVHVAIDSAEEGVAVLDRIRPWLAPLAALSTNSPF
                     WLGEDSGYASFRSQVWQRWPSAGPYGPFGSIERYRAFVDSVLDTGTVLDEGMVYLDAR
                     LSRQYPTVEVRVADVCREPDDAVLIAALTRALVQTAVDAWRAGDQPDPVRTEVLRLAA
                     WRAGRSGLEGDLLDPGSWRPTPAAEVVARLVEHVRPALEVAGDVHTVRDLLAAVEARG
                     TGTRRQRKVYAKTGTLAAVVRDATDGTLVH"
     gene            1323..1931
                     /locus_tag="VFE39_02725"
     CDS             1323..1931
                     /locus_tag="VFE39_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET6257543.1"
                     /translation="MADWVFAIVDRLGAVGVGLLILLENLIPPIPSEVILPLAGFRAR
                     TGTLDVVAVWVAATAGSLLGALLLYGLGAWLGYERLRRLAGRRWFVFASQKDLERGRE
                     MFDRHGGKVVLLARCVPFLRSVVSIPAGIARMPLVRFAVLTAIGSGVWNAAFIALGWV
                     LGENWDSVQGWLGPVSYVVIGLVVVGLVILVARRLRSRSVTV"
     gene            2052..2852
                     /locus_tag="VFE39_02730"
     CDS             2052..2852
                     /locus_tag="VFE39_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HET6257544.1"
                     /translation="MDVATEQEWSEDRLHFAFGPGSDHPSELAGAIGPLAYEFVRLAE
                     MLAGESTVRGVLQRVVDAARAAVPGADVVSVTLRTPGGYYTPVETDALATRLDEVQYE
                     LDEGPCVEATRTAGLGMTFSADLGAGLEYPRFGPAAAHLGVGSVLAVGLFPHGGDTPR
                     MGALNVYSRKVDGLNELDRDIAFVLAAHASTALSATMASTAAELESAQLRQALSSRDV
                     IGQAKGILMERRKVSAGEAFDTLRAASQALNIKLSKVAQTLVERRADI"
     gene            2897..3328
                     /locus_tag="VFE39_02735"
     CDS             2897..3328
                     /locus_tag="VFE39_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6257545.1"
                     /translation="MLRTEVIRLAAVRKRYRGHGEVLAGVDLEVLPGCPVAVAVVGRN
                     GTGKSTLLRIAAGCAAPTAGTVSGLPRVVGFLPGRFPASTRMPVRAYLRHLAALHGVP
                     PADAVRDADNLLDALGSPATRTPRSPCSRRGTRRRWASRRR"
     gene            3307..3768
                     /locus_tag="VFE39_02740"
     CDS             3307..3768
                     /locus_tag="VFE39_02740"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257546.1"
                     /translation="MGLAQALSCRADLLVLDEPWSGLDATSAAALDARLAAESARGAG
                     MLVADHTGRAGTLPGVTVLRLVDGVLTPVAVGQPVDGAHTVVELRCPGDSADALRALA
                     PAGESWTGRGQLTVRVPAERGDALLAATLAAGCSVLGARRVACGVWREDAR"
     gene            3885..4166
                     /locus_tag="VFE39_02745"
     CDS             3885..4166
                     /locus_tag="VFE39_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257547.1"
                     /translation="MLLLGGVAHLACAAAGTAVGLLCARPLVERIGWSFCLGVAIVVV
                     TAVQPWLPPVGVAVRALTTVGPPPMVAAALGLVLAAGAAVVAWVVERRR"
     gene            4255..4566
                     /locus_tag="VFE39_02750"
     CDS             4255..4566
                     /locus_tag="VFE39_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6257548.1"
                     /translation="MVGVKDDGRPLFAQIAEQIESSIVDGSLAEETQVPSTNELAAFH
                     RINPATAAKGVNQLVADGVLYKRRGIGMFVATGARAQLLERRREDFARQYLGPLLAEA
                     R"
BASE COUNT          580 a         1629 c         1722 g          712 t
ORIGIN      
        1 gaatccgggc cgtgcgccgc gaaggagatc gggcgcacgg ccctacgcag ttccgccata
       61 ggttggcttc atcctgcggt gcggcaggta gttgcgggcg aagctgcggc cgcgcacccc
      121 gagcatcggg ccgatcccgc cgcggttgtt gacgagcacg atgactagtg gacgagagtg
      181 ccgtccgtcg cgtcacgcac caccgcggct aacgtgcccg tcttcgcgta caccttccgt
      241 tgccgtctgg tgcctgtgcc gcgcgcctcg accgcggcca gcaggtcgcg gacggtgtgt
      301 acgtcgcccg ccacctccag ggcgggccgg acgtgttcga ccagccgtgc cacgacctcg
      361 gcggccgggg tgggccgcca gctccctggg tcgagcaggt cgccctccag cccggaacgg
      421 cccgcccgcc aggccgcgag ccggagcacc tcggtccgca ccgggtcggg ctggtcgccc
      481 gcgcgccacg cgtccacggc ggtctggacg agtgcgcggg tgagcgccgc gatcagcacg
      541 gcgtcgtcgg gttcccggca cacgtccgcg acacgcacct cgaccgtcgg gtactggcgc
      601 gacagccggg cgtcgaggta gaccatcccc tcgtccagca cagtcccggt gtcgagcacg
      661 gagtcgacga acgcccggta gcgctcgatc gatccgaagg gcccgtacgg ccccgctgac
      721 ggccaccgct gccacacctg gctgcggaag ctggcgtagc cggagtcctc tcccagccag
      781 aacggcgagt tggtgctgag cgccgcgagg ggagccagcc acggccggat gcggtccagg
      841 acagcgaccc cctcctcggc ggagtcgatc gccacgtgca cgtggcagcc gcaggtgagc
      901 tgctcggaca ccgtgcgccc gaaacggcgg gccatctcct cgtagcggcg cgacggcgac
      961 acggtcggct cgacggccag cggggacgtg gccagcggag cgagcgccac gcccacgtcg
     1021 ccggctgccg ctgctgccgt cgcccgcgcc gagcgcagct cggcgctgag ctcgtcgagc
     1081 gtgtggcagg gcttcgtgcc ggtctccagc tgctcccggt gtagctcggc ggccaggtcg
     1141 tcctccgcgt cctcggccgc ggaggtggcg agcaccgcgt ggcccaccgc cctcggtcga
     1201 ccagtcgcgg ggtcgaccag caggaactcc tcctcgacgc ccaccgtccg taccgccacc
     1261 cacaggagta tggcggcgag cgcaagcggt tcgatttcgg atgtctcggg cactctggac
     1321 ccgtggccga ctgggtgttc gcgatcgtcg accgtctcgg agcagtgggt gtcggcctgc
     1381 tcatcctctt ggagaacctg atcccgccga tcccgtccga ggtgatcctg ccgctcgcgg
     1441 ggttccgggc ccgcaccgga acgttggacg tcgtcgccgt gtgggtcgct gccaccgccg
     1501 gttcgctcct gggggcgctg ctgctctacg gcctcggggc gtggctcggc tacgagcggt
     1561 tgcgtcgcct ggccggtcgc cgctggttcg tgttcgccag ccagaaggac ctcgaacgcg
     1621 gccgggagat gttcgaccgg cacggaggca aggtggtgct gctcgcgcgc tgtgtgccct
     1681 ttctgcggag cgtcgtgtcg atcccggcgg gcatcgcgcg gatgcccctg gtccggttcg
     1741 ccgtgctcac cgcgatcggc agcggcgtct ggaacgcggc gttcatcgcg ctcgggtggg
     1801 tcctcggcga gaactgggac agcgtccaag gctggctcgg accggtcagc tacgtggtga
     1861 tcgggctcgt cgtcgtcggg ttggtgatcc tcgtggcccg caggctgcgc tcgcgctccg
     1921 tcaccgtctg aacccgcgga tcccccgttc ggcggccggg cttcccccgg ttgccgtcga
     1981 aacgtgctgg cggcttgctc gacggcgcga tctgggtcaa gcttccgcgc cggttgaaga
     2041 ggagtatcga ggtggatgtg gcgacggaac aggaatggtc cgaggaccgg ttgcacttcg
     2101 ccttcgggcc agggtccgac cacccctcgg agctagcagg ggcgatcggt ccgctggcgt
     2161 acgagttcgt gcgcctggcc gagatgctcg cgggcgagtc gacggtgcgg ggcgtgctgc
     2221 agcgggtcgt ggacgccgcc cgcgccgcgg tgccgggcgc tgacgtcgtg agcgtcacac
     2281 tgcgcacgcc aggggggtac tacaccccgg tcgagaccga cgcgctcgca acgcggctgg
     2341 acgaggtgca gtacgagctg gacgagggcc catgcgtcga ggccacccgc acagcgggac
     2401 tcggcatgac gttcagcgcc gacctcggtg ccggcctcga gtacccccgg ttcggcccgg
     2461 ccgccgctca tctgggtgtc ggcagcgtgc tcgccgtcgg tctgtttcca cacggtggcg
     2521 acacgccgcg gatgggcgcc ttgaacgtct actcgcgcaa ggtcgacggc ctcaacgagc
     2581 tggaccgtga catcgcgttc gtgctggcgg cgcacgcgtc caccgcgctc tccgccacca
     2641 tggcgagcac ggcggccgag ctggagtcgg cccagctgcg ccaggctctg agcagccgtg
     2701 acgtcatcgg tcaggcgaag ggcatcctca tggagcgccg gaaggtctcc gcaggcgagg
     2761 cgttcgacac actgcgcgcg gcgtcacaag cgctcaacat caagctgtcg aaggtggctc
     2821 agacactcgt cgagcggcgc gccgacatct gaaccggcgc cgcggcgccc gcagccggtt
     2881 gcggcaccat gccccagtgc tgcggaccga ggtgatccgg ctcgcggcgg tgcgcaagcg
     2941 ctaccgcggc cacggcgagg tcctcgccgg cgtggacctc gaagtcctgc cgggctgtcc
     3001 cgtggccgtg gccgtggtcg gccggaacgg gaccggcaag tccacgctcc tgcggatcgc
     3061 ggccgggtgc gcggccccga cggccggcac ggtctcaggg ctgccgcggg tcgtcggttt
     3121 cctgcccggc cgatttccgg cctcgacgcg gatgcccgtg cgggcctacc tgcgccacct
     3181 ggccgcgctg cacggcgtgc cgcccgcgga cgccgtgcgt gacgccgaca acctgctcga
     3241 cgccctgggt tcaccggcga cccgaacgcc ccggtcgccc tgctctcgac ggggaacgcg
     3301 cagaaggtgg gcctcgcgca ggcgttgagc tgccgggcgg acctcctggt gctggacgag
     3361 ccgtggtcgg gtctggatgc gacgtcggcc gcggcactgg acgcccgact cgcggccgag
     3421 agcgcccgcg gcgccgggat gctcgtcgcc gaccacaccg ggcgggcggg cacactgccc
     3481 ggagtgacgg tgctgcgcct ggtggacggt gtgctcactc ccgtcgcggt gggccagccg
     3541 gtggacgggg cgcacaccgt cgtcgagctg cgctgtcccg gcgatagtgc cgacgccctg
     3601 cgcgcgcttg cgcccgccgg ggagtcctgg accggccggg ggcagttgac cgttcgggtg
     3661 ccggcggagc gcggcgacgc cctgctcgcg gcgacgctcg cggcaggctg ctcggtgctc
     3721 ggtgctcggc gtgtggcgtg tggcgtgtgg cgtgaggatg cccggtgagc gaggcgcgat
     3781 gagcggaggc cccgcccgca cgctggccgt tgcccggttc ctggccgcgg acgtgtcccg
     3841 ctcgcagcgg tacctgctgc cgctccaagc tctacccgcc gtcggtgctg ctgctcggcg
     3901 gtgtcgcgca tctcgcttgc gccgccgcag gcactgcggt cgggctcctg tgtgcgcgcc
     3961 cgctggtcga gaggatcggc tggtcgttct gcctcggtgt agcgatcgtc gtcgtgacgg
     4021 ccgtccagcc gtggctgccg ccggtgggcg tcgccgtgcg tgcgctcacg accgtcgggc
     4081 cgccgccgat ggtcgcggct gcgctgggcc tcgtcttggc ggcaggggcc gccgttgtcg
     4141 cgtgggtggt cgagcgccgc cgctgacggg tcccgtccct tgtcgcttga cactcggctc
     4201 cgttgccttc atggttagct acatgagtaa tgaaccgacg aaccgacgcg atcggtggtc
     4261 ggtgtgaagg acgacgggcg cccgctgttc gcgcagatcg ccgaacagat cgagagctcg
     4321 atcgtcgacg gatcactggc cgaggagacg caggtgccct cgaccaacga gctggctgcc
     4381 ttccaccgca tcaatcccgc cactgcggcg aagggcgtca accagctcgt ggccgacggg
     4441 gtgctctaca agagacgggg gatcgggatg ttcgtcgcga ccggggcccg cgcgcagctg
     4501 ctggagcgcc gccgcgagga cttcgcgcgg cagtacctgg gcccgctgct ggcagaggcc
     4561 cgctagctcg gcaacgacac cgagcagatc aagaagatga tcgatgtctg gggggacgag
     4621 agatgaacgt cgtcaggatg agc
//
LOCUS       DASRSC010000053         6580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1818512,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000053 DASRSC010000000
VERSION     DASRSC010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1818512"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..129
                     /locus_tag="VFE39_02755"
     CDS             <1..129
                     /locus_tag="VFE39_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HET6257549.1"
                     /translation="AQAACAAALPPDRLPAPGAPVLDLMAQAHVHHHREEVRLFVS"
     gene            212..928
                     /gene="ureG"
                     /locus_tag="VFE39_02760"
     CDS             212..928
                     /gene="ureG"
                     /locus_tag="VFE39_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HET6257550.1"
                     /translation="MSTDHADGHGHGHGYSLDPTATEPDPHVPLTSSGRAVRIGIGGP
                     VGSGKTALVAALARALGSQLRLGVVTNDIYTTEDADFLRRAGVLTPDRIEAVQTGCCP
                     HTAIRDDITANLDAVELLEERFGDLDLVLVESGGDNLTAVFSRGLIDRQIFVVDVAGG
                     DKVPRKGGPGVTTADLLVINKVDLAPYVGADLDVMTSDAHRMRRGLPVITQSLVTDPA
                     ATDVAEWVLAQLVAVRAPAA"
     gene            935..1711
                     /locus_tag="VFE39_02765"
     CDS             935..1711
                     /locus_tag="VFE39_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HET6257551.1"
                     /translation="MRARSRIVVEWDGGRSVVRELRSQPPLSLLPQRGTAASRSPIAT
                     VHMVGSATTPLGGDDVELEVVVGPGADLVLTGVAATLALPGQGEPTSRLVVRLRIGDG
                     GGLQYLPEPTVITRRANHHAELHAELGAGARLRCREVLVAGRNVETSGRFRGHIRVVD
                     AAAGRPLLVQAQELGDQTLHDSAAHLAGRRVLGTEVLVWGEDPSTGLAGAWWSLTPLA
                     GRGSLASAVGADAVTTGRGLARAIAGHPGWTATTLTTAAP"
     gene            complement(1862..2524)
                     /locus_tag="VFE39_02770"
     CDS             complement(1862..2524)
                     /locus_tag="VFE39_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6257552.1"
                     /translation="MHLLVRCLLAPFARFVYRPVVDGAHRVPRSGPVIIAANHRAAVD
                     TAVIALTAGRPVSFLGKAEYFSRSGLRGRALASFLSALGYVPVQRGNAKAGLDALHAA
                     RTVLDAGGAFAIYPEGTRSLDGRLHRGHTGVATLALRTGAPVVPVALIGTERVQPVGA
                     RFPRLRRVTVRFGEPLDFGRYEGLENSSMIRRAVTDEVMDAIASLSQQEYVDTYHERP
                     AA"
     gene            2694..4301
                     /locus_tag="VFE39_02775"
     CDS             2694..4301
                     /locus_tag="VFE39_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005150194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET6257553.1"
                     /translation="MPAALLAHDLVKTYRDRTVLDGVSLTAAPGSRIGLIGENGAGKS
                     TLLRLLAGVEEPDSGTVSRPPDLGYGHQELPYSGHARIADVLDDALRESRSAVRALDA
                     LSATLADHPDDPDALRHYGDCLEWCHDHDAWDADRRAVLVLAGLGLAAVGTDRRLDTL
                     SGGQRGRLALAALLVRRPGALLLDEPTNHLDDDAAGFLEDQLRRLPGVVVAASHDRTF
                     LDAVCTDVVDLDPALCGPTRHGGGFTDYLDARRAGRARWEQRYAAEQDELQVLRHAVA
                     VTARRVAHDRAPRDNDKMAYDFFGGRVQRQLSRRVRNAERRLDELTREQVRRPPEPLR
                     FAATVGTPASGPVAALRRVHVRDRLVLDHLDIAANDRLLVTGPNGAGKSTLLAVLAGR
                     LDPADGVVHRRRGLRVGLLAQDIAIEQPTRTARAVYADRLGAERAAATPLATLGLLAP
                     KDVDRPVVELSVGQRRRLELALLVADAPQLILLDEPTNHLSPALVEEVEEALDSSRGA
                     VVIASHDRWLRRRWAGRELRLADGRPA"
     gene            complement(4307..5311)
                     /locus_tag="VFE39_02780"
     CDS             complement(4307..5311)
                     /locus_tag="VFE39_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HET6257554.1"
                     /translation="MAAGLAWFVAHDLVGHQRPFFAPIAAVISLGVSLGNRLRRVVEL
                     VVGVSLGVLVADLLIARIGSGAWQIILVVALAMAIAVFADGGTLLVAQAGSSAVLVAT
                     LLPPGQAGGLDRCVDALIGGAIGVLVAAILPTDPVGPVRRQARVLLEELAAVLASLAD
                     ALRNRDADAAAEALQRARASQPLIDELRAALRGGHEVTTLSPLHRRRRRVLGRYAELA
                     ERADYAMRNARVLARRAYTALENGEPVAPELADLLGELGRAVRVLTDQLGRDGDRERA
                     REPVLDVVRHSVALSDEWEGGPSEQVMVAQLRSIALDLLQATGMTRAEALAAMRRQPS
                     "
     gene            complement(5409..5822)
                     /locus_tag="VFE39_02785"
     CDS             complement(5409..5822)
                     /locus_tag="VFE39_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3151 domain-containing protein"
                     /protein_id="HET6257555.1"
                     /translation="MTLHGNLLGPEPTLLPVDPAAAELDRGVQPEEVAAAHPTSSLAW
                     AVLAERALEDALTITAYAYARTGYHRGLDQLRRSGWKGFGPVPWSHEPNRGFLRALGA
                     LQRAAALIGETDEAERCRVFLAESDPAAPAALGLD"
     gene            complement(5896..>6580)
                     /gene="fbaA"
                     /locus_tag="VFE39_02790"
     CDS             complement(5896..>6580)
                     /gene="fbaA"
                     /locus_tag="VFE39_02790"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HET6257556.1"
                     /translation="VDRGENPLFQSHMWDGSATELHENLRIAAELLDQAVKAKIILEV
                     EIGVVGGEEDGVANDINEKLYTAPGDYEATVEALGGGEKGRYLLAATFGNVHGVYKPG
                     NVKLRPEILKQGQEVATAKLGLPENSKPLDLVFHGGSGSLLEEIHEALSYGVVKMNVD
                     TDTQYAFTRPIADHMFRNYDGVLKVDGEVGNKKAYDPRTYLKAAEVGMAERVTLACEN
                     LRSTGTTLR"
BASE COUNT          909 a         2404 c         2403 g          864 t
ORIGIN      
        1 gcgcaggccg cttgcgccgc tgcgcttccg ccggaccggc tgcccgcacc cggcgctccc
       61 gtcctcgacc tcatggctca ggcccacgtc caccaccacc gggaagaggt gcgtctcttt
      121 gtctcctgaa cggagctcgc ggagtgacca tcaacgccga acggagcagg cgcagtgacc
      181 atcaacgccg aacggagcag gcgcagtgac catgagcact gaccacgcag atggccacgg
      241 ccacggccac ggctactcgc tcgaccccac ggccacggag cccgacccgc acgtcccgct
      301 cacctcgtcg gggcgggcag tgcggatcgg catcgggggc ccggtcggga gcgggaagac
      361 ggcactggtc gcggcgctcg cccgcgcgct cgggtcgcag ttgcggctcg gggtggtgac
      421 caacgacatc tacaccaccg aggacgccga cttcctacgc cgggcaggcg tgctcacccc
      481 ggaccgcatc gaggccgtcc agaccggctg ctgcccgcac accgcgatcc gcgacgacat
      541 cacggccaac ctcgacgccg tcgagctgtt ggaggagcgg ttcggtgacc tcgacctggt
      601 gctcgtggag agcggcggcg acaacctgac ggccgtgttc agccgcgggc tgatcgaccg
      661 gcagatcttc gtcgtcgacg tggcgggcgg ggacaaggtg ccgcgcaagg gcggtcctgg
      721 cgtcaccacc gccgacctgc tggtgatcaa caaggtggac ctcgccccgt acgtcggcgc
      781 ggacctcgac gtgatgacct ccgacgcgca ccggatgcgc cgagggctgc cggtcatcac
      841 gcagtccctg gtaacggacc cggccgccac cgacgtcgcc gagtgggtgc tcgcccagct
      901 cgtcgccgtg cgcgccccgg ccgcctgacg acgcgtgcgg gccaggagcc gcatcgtcgt
      961 cgagtgggac ggcggacgca gcgtggtgcg ggagctgcgg tcgcagccgc cgctgtcgct
     1021 gctgccccag cggggcacgg ccgcgtcccg ctcgccgatc gccacggtgc acatggtcgg
     1081 ctcggccacc actccactgg gcggcgacga cgtggagctc gaggtcgtcg tcgggccggg
     1141 cgcggacctc gtgctcaccg gggtggccgc cacgctcgcc ctgccagggc agggcgagcc
     1201 caccagccgg ctcgtcgtcc ggctccggat cggcgacggt ggcgggctcc agtacctgcc
     1261 ggagccgaca gtgatcaccc gccgcgccaa ccaccacgcc gagctccatg ccgagctggg
     1321 agcaggcgcg cggttgcgct gccgggaggt gctcgtcgcc ggccggaacg tggagacctc
     1381 ggggcgcttt cgggggcaca tccgcgtggt cgacgccgca gcggggagac ccctgctcgt
     1441 gcaggcacag gagctgggcg accagacact gcacgactcc gccgcgcacc tcgccgggcg
     1501 ccgcgtgctg ggcacggagg tgctggtgtg gggcgaggac ccgagcacgg gtctggccgg
     1561 tgcttggtgg tcgctcaccc cgctcgccgg gcgtggatcc ctcgcctcgg ccgtgggagc
     1621 ggacgctgtc acgacgggcc ggggcctcgc acgggcgatc gccggtcacc ccggctggac
     1681 ggctacgacg ctgaccacgg cggccccctg agccgctccg ggcgcctccc gtggtcagcg
     1741 gatggtgtgg cagctcactg tccgccggta cgcccgacga caacgccgcg agggtgccgc
     1801 ctgggtgccg cctgggtgcc gcgcagtagg gccgagttgt tcagcgagcg ttcttggcca
     1861 gtcaggcggc ggggcgctcg tgatacgtgt ccacgtactc ctgctgggag aggctcgcga
     1921 tcgcgtccat gacctcgtcg gtgaccgcgc ggcggatcat gctcgagttc tccagcccct
     1981 cgtagcggcc gaagtcgagc ggctcgccga agcgcaccgt cacgcggcgc aggcgaggga
     2041 accgggcccc gacgggctgc acccgctcgg taccgatcag cgccaccggg accacaggcg
     2101 cgccagtgcg cagcgcgagg gtggcgaccc cggtgtgccc acggtgcagc cgcccgtcga
     2161 gcgagcgggt gccctccggg tagatggcga atgcgccgcc ggcatccagc acggtgcgcg
     2221 cggcgtgcag ggcgtcgagc ccggccttgg cgttgccccg ctggacgggg acgtagccca
     2281 gcgccgagag gaacgacgcg agcgcgcgcc cgcggaggcc ggagcgactg aagtactcgg
     2341 ccttgccgag gaacgagaca ggacgaccgg ccgtcaatgc gatcaccgcc gtgtcgacgg
     2401 ccgcgcgatg gttcgccgcg atgatcaccg gcccgctgcg gggcacccgg tgcgcgccgt
     2461 ccaccacagg ccgatagacg aaccgggcga atggcgcgag gaggcagcga accagcagat
     2521 gcacggcgca agaacgctgg tgatccccag ccggttcccg cacggggaaa ccttgttgca
     2581 ggaccacccg gatggcggca ttctggcggt gtcatgcgga tctaggtgcg ccagcacatc
     2641 cacgtccgag gcatcgcccg gtgggtgtcc cgtcgacccc tcggtggttc cgcgtgcccg
     2701 cagcacttct cgcccacgac ctcgtcaaga cctaccgcga ccgcactgtc ctcgacggcg
     2761 tgtcgctaac agcggcgccc gggagccgga tcgggctcat cggtgagaac ggagccggca
     2821 agtcgacgct gctgcgactg ctcgcaggcg tggaggagcc cgactccggc accgtctcgc
     2881 gtccgcccga cctcgggtac ggccaccagg agctgccgta ttcggggcac gcgcggatcg
     2941 cggatgtgct cgacgacgcg ctacgggagt cccgttccgc cgttcgcgcg ctggacgccc
     3001 tgtccgcgac gctcgcggat catcccgacg accccgacgc actgcgccac tacggagact
     3061 gcctcgagtg gtgccacgac cacgacgcgt gggacgccga ccgccgcgcc gtgctggtgc
     3121 tggccgggct cggcctcgcc gcggtcggca cggaccggcg gctggacacg ttgtccggcg
     3181 ggcagcgggg ccgactcgcg ctcgccgcgc tgctcgtacg ccgtcccggc gcgctactgc
     3241 tcgacgagcc gaccaaccac ctcgacgacg acgcggccgg attcctggag gaccagctcc
     3301 ggcgactgcc cggggtagtg gtcgcagcga gccacgaccg gacgttcctg gatgcggtgt
     3361 gcacggacgt ggtcgacctc gatccggcac tctgcgggcc gacgcgtcac ggcggcgggt
     3421 tcaccgatta cctggacgcc cgccgggcgg ggcgggcccg gtgggagcag cgctacgccg
     3481 cggagcagga cgagctgcag gtgctgcggc acgcggtggc ggtcaccgcg cgccgagtcg
     3541 ctcacgaccg ggcgcctcgc gacaacgaca agatggccta cgacttcttt ggcggacggg
     3601 tgcagaggca gctgtcccga cgggtgcgca atgccgagcg acggctcgac gagctcaccc
     3661 gcgagcaggt gcggcggccg ccggagccac tgcggttcgc cgccacggtc ggaactccgg
     3721 cgagcgggcc cgtcgcggcg ctgcgccgcg tgcacgtccg ggacaggctg gtcctcgacc
     3781 atctcgacat cgccgcgaac gaccggctgc tcgtcaccgg gcccaacggg gcagggaagt
     3841 ccacactgct cgcggtgctg gccggccgcc tggacccggc ggacggcgtg gtgcaccgac
     3901 ggcgcgggct gcgcgtcggc ctgttggccc aggacatcgc gatcgagcag ccgactcgta
     3961 cggcacgcgc ggtgtacgcc gaccgcctcg gcgcggagcg ggcggccgcc accccgctcg
     4021 cgacgctcgg gctgctcgcg ccgaaggacg tcgaccggcc tgtcgtggag ctctcggtcg
     4081 ggcagcggcg acggctggag ctcgctctgc tggtggccga tgcgccgcag ctgatcctcc
     4141 tcgacgagcc gaccaaccac ctctcacccg cgctcgtcga agaggtggag gaggcactgg
     4201 actcgagccg gggcgcggtg gtgatcgcga gccacgaccg gtggctgcgc cggcgctggg
     4261 cgggtcgcga gctgcgcctg gcggacggcc gcccggcctg accgcatcag gagggttgcc
     4321 gccgcatcgc cgccagggcc tcggcgcggg tcatgccggt cgcctgcagc aggtccagcg
     4381 cgatcgagcg cagctgcgcg accatcacct gctccgacgg ccccccttcc cactcgtccg
     4441 acagcgcgac cgaatgccgg acgacgtcga gcaccggttc gcgggcccgt tcccggtcgc
     4501 catcgcgccc tagctggtcg gtgagcaccc ggaccgcccg gcccagctcc cccaggaggt
     4561 ctgcgagctc cggcgcgaca ggttcgccgt tctccagggc ggtgtaggcg cggcgggcga
     4621 ggacgcgggc gttgcgcatc gcgtagtcgg cgcgctcggc gagctccgcg tagcgaccga
     4681 ggacgcggcg acggcggcgg tgcaacggcg agagcgtcgt gacctcatga ccgccgcgca
     4741 gcgccgccct cagctcgtcg atcagcggct ggctggcgcg ggcccgctgc agcgcctctg
     4801 cggcggcatc ggcgtcacgg ttgcgcagcg cgtcggcgag actggcgagc acggcggcga
     4861 gctcctcgag caggacgcgc gcctggcggc gcaccggccc taccgggtcc gtcggcagga
     4921 tggccgcaac cagcaccccg atcgcgccgc cgatcagggc gtcgacgcag cggtcgagcc
     4981 cgccggcctg gccgggcggc agcagggtcg ccaccagcac cgcggacgac ccggcctgcg
     5041 cgacgagcag ggtgccaccg tcggcgaaca ccgcgatggc catcgcgagc gcgacgacca
     5101 ggatgatctg ccaggctccg ctcccgatcc gggcgatcag caggtcggcg acgagtacgc
     5161 cgaggctcac cccgaccacc agctcgacca cgcggcgcag cctgttgccc agcgagacgc
     5221 cgaggctgat caccgccgca atcggggcga agaacggccg ttggtgaccg accaggtcgt
     5281 gggcgacgaa ccaggcgagg cccgccgcca cggcgcactg tgcgatcgga agcgcggaca
     5341 gcctgacccg acgagcgcct tgttggagct gggcgcgcag ccggtccgcc cggaccgcag
     5401 gcaacgagtc agtccaggcc gagggcagcg ggcgcggccg ggtcggactc ggcgaggaac
     5461 acgcggcagc gctccgcctc gtcggtctcg ccgatgaggg ccgcggcgcg ctggagcgcg
     5521 cccagtgccc gcaggaagcc ccggttgggc tcgtgcgacc acggaaccgg gccgaatccc
     5581 ttccagcccg agcggcgcag ctggtcgagg ccgcggtggt agccggtgcg cgcataggcg
     5641 tatgcggtga tcgtcagggc gtcctcgaga gcccgctcgg cgagcaccgc ccacgccagg
     5701 gacgacgtcg ggtgcgccgc cgcgacctcc tcgggctgga cgccgcggtc gagctcggcc
     5761 gcggccgggt cgaccggcag cagggtgggc tccggtccga gcagattgcc gtgcagggtc
     5821 atgccagcat cttgccgtcc tccaggtcgt gcgcggaaac gagtgccgca gcacccgtat
     5881 ccgcgcacga gcgcgtcagc gcagagtggt gcccgtcgac cgcaggttct cgcaggcgag
     5941 ggtgacccgc tcggccatcc cgacctcggc cgccttgagg tacgtgcggg ggtcgtaggc
     6001 cttcttgttg ccgacctcgc cgtcgacctt gagcacgccg tcgtagttcc tgaacatgtg
     6061 gtccgcgatc ggccgcgtga aggcgtactg cgtgtccgtg tcgacgttca tcttgacgac
     6121 gccgtagctc agcgcctcgt ggatctcctc gagcagcgag cccgaaccgc cgtggaagac
     6181 caggtcgagc ggcttcgagt tctcgggaag cccgagcttc gcggtggcca cctcctggcc
     6241 ctgcttgagg atctccgggc gcagcttcac gttgcccggc ttgtagacgc cgtgcacgtt
     6301 gccgaacgtg gcagccagca ggtagcgacc cttctcgccg ccgcccagcg cctccaccgt
     6361 ggcctcgtag tcgcccggcg cggtgtacag cttctcgttg atgtcgttgg cgacgccgtc
     6421 ctcctcgccg cccaccacgc cgatctcgac ctcgaggatg atcttcgcct tgacggcctg
     6481 gtcgagcagc tccgcggcga tccgcaggtt ctcgtgcagc tcggtggccg acccgtccca
     6541 catgtgcgac tggaacagcg ggttctcccc gcggtcgacc
//
LOCUS       DASRSC010000054         2101 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5585595,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000054 DASRSC010000000
VERSION     DASRSC010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5585595"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="VFE39_02795"
     CDS             <1..1587
                     /locus_tag="VFE39_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET6257557.1"
                     /translation="AGGGGETGGSMDAGNMLKPALARGELHVIGATTHDEYRRHIESD
                     AALARRFQPVMVPEPSCEDAVEILNGLRDRYEAHHQVRYTEDALRAAVELSERYVTER
                     FLPDKAIDLMDQAGARVRLRTGTPSNDLRGLEQRLEQLERDKDQAVTDEQYERATELR
                     DQIAELRKRLTSADSADDCVPEVGVTEIADVVSRQTGVPVAQLTESERDRLLRLEQEL
                     HIRLVGQDEAVTAVAEAIRRSRAGLGDEDRPVGSFLFLGPTGVGKTELARALAVALFG
                     EEDRMVRLDMSEYGERHTVARMIGAPPGYVGYGEAGQLTEAVRRRPYSVLLLDEIEKA
                     HPDVFNVLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNVGSELITSRGATLGFGSAADT
                     AEQDDAALRDRLMPRLREVFRPEFLNRIDEIIVFRRLDSEQLATVTDLLLEETRRRLG
                     AQDVTIEFTPAAVRRLAELGYQPEFGARPLRRTIQREVDNRLSGLLLAGKLAPGSHVR
                     VDARDGEFDLEVGGEPASVS"
     gene            complement(1605..>2101)
                     /locus_tag="VFE39_02800"
     CDS             complement(1605..>2101)
                     /locus_tag="VFE39_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674762.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HET6257558.1"
                     /translation="EGPPTAWLMLVGEQPGDKEDIAGEPFVGPAGRILDTALERAGIE
                     RAEVYLTNAVKHFRFERRGNRRLHKTPGVTHIRACRPWLDGELRAVRPAAVATLGAIA
                     AKALLGPKFRITSQRGKVLDWEGHRLVATVHPSAILRGPVEARDEALAALVADLQVVA
                     ALRP"
BASE COUNT          313 a          746 c          769 g          273 t
ORIGIN      
        1 gcgggcggcg gcggcgagac cggtggctcg atggatgcgg gcaacatgct caagcccgcc
       61 ctcgcacgcg gcgagctgca cgtcatcggc gccaccaccc acgacgagta ccgcaggcac
      121 atcgagtccg acgctgcgct cgcccgccgg ttccagccgg tcatggtgcc cgaaccgagc
      181 tgcgaggacg ccgtcgagat cctgaacggg ctgcgcgacc gctacgaggc ccaccatcag
      241 gtgcgctaca ccgaggacgc actgcgcgcc gccgtggagc tgtccgagcg ttacgtcacc
      301 gagcggttcc tgccggacaa ggccatcgac ctgatggacc aggccggtgc cagggtgcgg
      361 ctgcgcaccg gcacgccgtc gaacgacctg cgtgggctgg agcagcgcct cgaacagctc
      421 gagcgggaca aggaccaggc cgtgaccgac gagcagtacg agcgtgccac cgagctgcgc
      481 gaccagatcg ccgagctgcg caagaggctc acctcggccg acagtgcgga cgactgcgtc
      541 cctgaggtcg gggtcacgga gatcgccgac gtcgtctcgc gtcagaccgg cgtgccggtg
      601 gcccagctca cggagtccga acgcgaccgg ctgctgcggt tggagcagga gctgcacatc
      661 cggctggtgg gccaggacga ggccgtgacc gccgtggccg aggcgatccg ccgctcgcgg
      721 gccggactgg gcgacgagga ccgtccggtc ggcagcttcc tgttcctcgg cccgaccggg
      781 gtcggcaaga ccgagctggc cagggcgctc gcggtcgcgc tgttcggcga ggaggaccgg
      841 atggtccgcc tggacatgag cgagtacggc gagcggcaca ccgtggcgcg gatgatcggg
      901 gcgccgcccg gctacgtcgg ctacggggaa gcgggccagc tcaccgaggc cgtccggcgc
      961 aggccgtact cggtgctgct gctcgatgag atcgagaagg cgcacccgga tgtgttcaac
     1021 gtgctgctcc aggtgctcga cgacgggcgg ctgacggacg ggcagggccg cacggtcgac
     1081 ttccgtaaca ccgtgatcat catgaccagt aacgtgggtt cggagctgat caccagccgc
     1141 ggcgcgacgc tgggcttcgg gagcgcggcc gatacggccg agcaggatga cgccgcgctc
     1201 cgcgaccggc tgatgcccag gctgcgggag gtgttccgcc cggagttcct caaccgcatc
     1261 gacgagatca tcgtgttccg caggctcgac tccgagcagc tcgccacggt caccgacctg
     1321 ctgctggagg agacgcggcg ccggctcggc gcgcaggacg tgacgatcga gttcacgccc
     1381 gcggcagtac ggcggctggc agagctcggg tatcagccgg agttcggggc acggcccctg
     1441 cggcgcacca tccagcgcga ggtggacaac cggctgtcgg gcctcctgct ggcgggcaag
     1501 ctggcgccgg gcagccacgt gcgggtggac gcccgcgacg gcgagttcga cctcgaggtc
     1561 ggcggcgagc cggccagcgt ctcctgaccg ccaagggcgc gtcctcaggg acgcagcgcc
     1621 gccaccacct gcaggtcggc caccagggcg gccagtgcct cgtcccgggc ctccaccgga
     1681 ccccgcagga tcgccgacgg gtgcacggtc gccaccagcc ggtggccctc ccagtcgagc
     1741 accttcccgc gctggctcgt gatccggaac ttgggaccga ggagcgcctt ggcggcgatg
     1801 gcgccgaggg tggccacggc ggcgggccgc accgcgcgga gctcgccgtc cagccacggc
     1861 cggcaggctc ggatgtgggt gacgcctggc gtcttgtgca accgccggtt gccccggcgc
     1921 tcgaaccgga agtgcttcac cgcgttggtg aggtacacct cggctcgctc gataccggcg
     1981 cgctccagcg ccgtgtcgag gatccggccg gccggaccga cgaacggctc gcccgcgatg
     2041 tcctccttgt cccccggctg ctcccccacc agcatcagcc atgccgtcgg cggaccctcc
     2101 c
//
LOCUS       DASRSC010000055         6356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1982039,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000055 DASRSC010000000
VERSION     DASRSC010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6356
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1982039"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="VFE39_02805"
     CDS             <1..410
                     /locus_tag="VFE39_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257559.1"
                     /translation="LIARRWHLPAGAATIGYAVAALISATLTGFERLAVPLALVAAGI
                     LVDLLARWLRPRADRRAAYWAFGAAAPVVTWAVYLGVASAAEGALPAIVEFWTGMPII
                     AGLLGWSLAALMLPNPITVGEPACSERAATTSG"
     gene            421..1743
                     /locus_tag="VFE39_02810"
     CDS             421..1743
                     /locus_tag="VFE39_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6257560.1"
                     /translation="MPAAKLPNIDLPGVELHRTTLPNGLRVLIVPDPATPVVGVALHV
                     DVGFRSEPEGRTGFAHLFEHLMFQGSESLEKLAHFRHVQGSGGVFNGSTHQDYTDYFE
                     VLPAAALERALFLEADRLRAPKLTAENLRNQVDVVKEEIRLNVLNRPYGGFPWILLPP
                     VLYDTFPNAHNGYGDFSELEQASLDDAAAFFDTYYSPANAQITVAGYLDVDETLRLVE
                     KHFGDIPARPAPVRPSFAEPRPGGERRQVATDAHAPLPALAVGYRLPDPATDLDGYLG
                     HALLGSVLGDGEAARLQRRLVHVDGLVTDISASAGLMGPLDARDPDTFTITAVHPSAV
                     DPDRVLAAVDEELDKLAAEGPSVDEIARQVARWSAALHQENDRVMYRMLGLGARELLY
                     GRAEIAVELPDRLAAVTPEQVRAAADALRSSGRGVLLVEPTAAQEGTE"
     gene            1740..3152
                     /locus_tag="VFE39_02815"
     CDS             1740..3152
                     /locus_tag="VFE39_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6257561.1"
                     /translation="MTTTAPETTGHAVPGENPAHRSAAEIGRTDAGPRALPPLGLTRA
                     VPLPDVEERTLDSGLRVLAAHRPGVPMVELRLRVPFADDAPEHAAVSELLSSTLLAGT
                     ASRDRVGIDDELAAVGASLGVSVDPERLQIGGSGLADGLPDLLAVLADVLTSATHPDT
                     EVARERDRLVERISVARAQPRTIAREALQRKRFGDHPISREMPAGEDVAIVTADQVRA
                     LQAASLVPRGSILTLVGDIDTAMAIGQVEKALAGWTSDAAARELSPPPAPPAADLQLV
                     HRPGSVQSQLRLSAPALRRHDPRYAAFQLANLVFGGYFSSRLMENIREDKGYTYGAHS
                     GIEFIPGGAVLGVETDVASDVTAAALLETRYELGRLTAVPPTEAEVEAARSYAVGSLL
                     ISLDNQGGMASMLSALAADGLGIEWLRGHPARLEAVTPEQVAEVALEFFAPTAFTGVV
                     VGDADVIGPGVRALGGITGP"
     gene            3149..4147
                     /gene="nudC"
                     /locus_tag="VFE39_02820"
     CDS             3149..4147
                     /gene="nudC"
                     /locus_tag="VFE39_02820"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HET6257562.1"
                     /translation="MSEPCTIGSGIDFTLLGPPVLSRSAVERDEPLRTNTERQIAGWP
                     QARLVVVDARGRTLVDWEGGPPQGGWDAVTGGGARLRTRPTTGDTPSDGAVLLGEGAG
                     IAYWAVRGEPDLAAGEDPGEWLDLRMAGAALDSLGAGLLTTAVAVLNWHDTAQFCSRD
                     GSPTHPSNAGWHRHCEAHGHEEYPRTDPAVICLVHDGRDRVLLARQPTWPEGRYSVLA
                     GFVEAGESLEACVLREIHEEVGATVRDVRYLGSQAWPFPRSLMVGFHAEADPDVPLVP
                     ADGEIAEAMWVTRAELRAALEHGDWAADGQQDARPLLLPGRVSIARSMLESWAALD"
     gene            complement(4221..4469)
                     /locus_tag="VFE39_02825"
     CDS             complement(4221..4469)
                     /locus_tag="VFE39_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycoredoxin"
                     /protein_id="HET6257563.1"
                     /translation="MADLTMYSTIWCGYCRRLKLQMDQAGIAYSEVDIERDPDAARFV
                     ESVNGGNQTVPTVVFADGTAMTNPPFAVVAEKVADLAA"
     gene            4525..>6356
                     /locus_tag="VFE39_02830"
     CDS             4525..>6356
                     /locus_tag="VFE39_02830"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HET6257564.1"
                     /translation="MTARLVPDVPESGLDDEQLAAVTAPRGPVCVLAGAGTGKTRTIT
                     RRIAHLVRTGHVAPGQVLAVTFTARAAGEMRTRLRTLGVAGVQARTFHAAALRQLRYF
                     WPRVVGGDQWQLLEGKLRIVGQAATRAKAGTDAASLRDLAGEIEWAKASLVTPEGYAA
                     AVAKLRRDIPAPAEQVSAVYAGYEALKNRAELLDFDDLLLHTAAALEEHGAVAEEFRD
                     RYRCFVVDEYQDVTPLQQRVLDAWLGDRDDLTVVGDANQTIYTFAGATPRHLLDFPRR
                     FPEAVVVRLQRDYRSTPQVVSAANTLISSARGRVAGTRLQLVGQLPPGREPDFAEHDD
                     EPAEAAAVAAQIKRLVAEGTPASEIAVLFRINAQSEVYEQALTDVGVPYQVRGGERFF
                     SRPEVRRAMTVLRAAALRTPDHDTAVVDTVRAVLAPVGLTPEPPGGAAQRAQWESLLA
                     IVELAEELVAVEPDADLRRFAAELDTRADAAHPPTVQGVTLASLHAAKGLEWDVVFLV
                     GLVDGTLPIQHADGDDEAIEEERRLLYVGITRARRRLALSWALSRQPGGPRRRRRSRF
                     LYGLVPEHHPASRIALGERGGAKPRCRVCGSPLVGVDALKLRRCG"
BASE COUNT          832 a         2358 c         2255 g          911 t
ORIGIN      
        1 tgctgatcgc ccgccgttgg cacctgcctg caggcgcggc gacgatcggc tatgccgtcg
       61 cggcgctgat ctctgcgacc ctcaccggct tcgagcggct cgccgtcccg ctggcgctcg
      121 tggccgctgg catcctcgtc gacctactgg cccggtggct gcgtccccgc gccgatcgtc
      181 gcgcggctta ctgggccttc ggcgccgcag cgccggtggt cacctgggcc gtgtacctcg
      241 gggtcgcctc cgccgccgag ggcgcgctcc ccgccatcgt cgagttctgg accggtatgc
      301 cgatcatcgc gggcctgctc ggttggtcgc tggctgcgct gatgcttccc aaccccatca
      361 ccgtcggtga gcccgcctgc tccgagcggg cggcgaccac ctcgggctag attcgggcgc
      421 gtgcctgccg caaaactgcc caacatcgac cttcccgggg tcgaactgca caggacgacg
      481 ctgcccaacg ggctgcgagt cctcatcgtc cccgacccag ccaccccggt cgtcggcgtc
      541 gcactgcacg tcgacgtcgg tttccgctcg gaaccggagg ggcgcaccgg cttcgcccac
      601 ctgttcgagc acctgatgtt ccagggcagt gagagcctgg agaagcttgc gcacttccgg
      661 cacgtccagg gctcgggtgg ggtgttcaac ggctccaccc accaggacta caccgactat
      721 ttcgaggtgc tgcctgccgc cgcgctggag cgggcgctgt tcctggaggc cgaccggctc
      781 cgtgcgccga agctgacggc tgagaacctg cgcaaccagg tggacgtcgt caaagaggag
      841 atccggctca acgtcctgaa ccggccctac ggtgggttcc cgtggatcct gctgccgccg
      901 gtcctctacg acacgttccc caacgcgcac aacggctacg gcgacttctc cgagctggag
      961 caggcatcgc tcgacgacgc agccgccttc ttcgacacct actactcgcc ggccaatgcg
     1021 cagatcaccg tggccggcta tctcgacgtc gacgagacgc tccggctggt ggagaagcac
     1081 ttcggcgaca tcccggcccg gccagccccc gtccgtccgt cgttcgcgga gccgcggccg
     1141 ggcggtgagc gccgccaggt cgccaccgac gcccacgccc ccctgcccgc gctcgccgtc
     1201 ggctaccggc tccccgaccc cgccaccgac ctggacggct acctcggcca cgcgctcctg
     1261 ggctccgtgc tcggcgacgg cgaggcagcc cggctgcagc gacggctcgt gcacgtcgac
     1321 ggcctcgtca ccgacatctc cgcgagcgcc ggactgatgg gcccgctcga tgcccgcgac
     1381 ccggacacgt tcacgatcac ggccgtgcac ccgtccgccg tcgaccccga ccgcgtgctc
     1441 gctgccgtcg acgaggagct cgacaagctc gccgccgagg gtccgagcgt cgacgagatc
     1501 gcccggcagg tggcgcgctg gtcggcagca ttgcaccagg agaacgaccg cgtgatgtac
     1561 cggatgctcg gcctcggcgc gcgcgagctg ttgtacggcc gcgcggagat cgccgtcgag
     1621 ctgccggatc ggctcgccgc cgtcaccccc gagcaggtac gggcggctgc ggatgccctg
     1681 cgctcgtccg ggcgcggggt cctgctcgtc gagccgaccg ctgctcagga aggcacggaa
     1741 tgaccaccac agcaccggag accaccggcc acgccgtccc cggggagaac cccgctcacc
     1801 gcagcgccgc ggagatcggc cggacggacg ccgggccgcg cgcactgccg ccgctcgggc
     1861 tcacccgcgc cgtcccgctc ccggacgtgg aggagcgcac gctcgactcc gggctgcggg
     1921 tgctggccgc acaccgtccc ggggtgccca tggtggagct gcggttgcgg gtcccgttcg
     1981 ccgacgacgc gcccgagcac gctgcggtga gcgagctgct ctcgtccacg ttgctcgccg
     2041 gaacagcgag ccgcgaccgc gtcggcatcg acgacgagct ggccgccgtg ggtgccagcc
     2101 tcggcgtgag tgtggatccg gagcggctcc agatcggcgg ctccgggctg gccgacggac
     2161 tgcccgacct gctcgcggtg ctggccgacg tcctcacctc ggccacccac cccgacaccg
     2221 aggtcgcgcg tgagcgcgac cgcctcgtcg agcggatctc ggtggcccgc gcgcagccgc
     2281 gcacgatcgc ccgggaggcc ctgcagcgca agcggttcgg cgaccacccg atcagccggg
     2341 agatgcccgc cggggaggat gtcgccatcg tcacggcaga tcaggtgcgg gcactccagg
     2401 ccgcatcgct ggtgccgcgg ggttcgattc tcaccctggt cggcgacatc gacaccgcca
     2461 tggcgatcgg gcaggtcgag aaggcactcg caggctggac gtcggacgcg gcggcccgcg
     2521 agctgtcccc gccccccgct cctcccgccg ccgacctgca gttggtgcac cgtccaggat
     2581 ccgtgcagtc gcagctgcgc ctgtcggcgc ccgcgctgcg ccgccacgac ccgcgctacg
     2641 ccgcgttcca gctggccaac ctcgtgttcg gcggctactt ctcctcgcgg ctgatggaga
     2701 acatccgcga ggacaagggc tacacctacg gcgcccactc ggggatcgag ttcatccccg
     2761 gaggcgcggt gctgggcgtc gagaccgacg tggcgagcga cgtcaccgcc gcggcgctgc
     2821 tcgagacccg ctacgagctg ggcaggctca ccgcggtccc cccgaccgag gccgaggtcg
     2881 aggccgcacg cagctacgcg gtgggctcct tgctcatctc cctggacaac cagggcggta
     2941 tggcgtcgat gctgtccgcg ctggccgcgg atgggctcgg gatcgagtgg ttgcgtgggc
     3001 atccggcgcg gctggaagcc gtcacgcccg agcaggtcgc cgaggtggca ctggagttct
     3061 tcgctcccac cgcgttcacc ggagtcgtcg tgggcgacgc cgatgtgatc gggcccggag
     3121 tgcgcgcgct cggcgggatc accgggccat gagcgagccg tgcacgatcg ggtccggcat
     3181 cgacttcacg ctgctcgggc cgccggtgct gtcccgctcg gccgtggagc gcgacgagcc
     3241 gctgcgcacc aacaccgagc ggcagatcgc gggctggccc caggcgcggc tcgtcgtggt
     3301 ggatgccagg gggcgcacgc tcgtcgactg ggagggcgga cctccgcagg gcggctggga
     3361 cgcggtgacc ggcgggggcg cgcggctgcg cacccgtccg accacgggag atacgccatc
     3421 ggatggtgct gtgctgctcg gggagggcgc gggcatcgcc tactgggcgg tgcgcggcga
     3481 gcccgacctg gctgcgggcg aggatccggg cgagtggctg gatctgcgga tggcgggggc
     3541 cgcgctcgac tcgctcgggg cgggcctgct gaccacggcg gtcgccgtcc tcaactggca
     3601 cgacacggcc cagttctgct ctcgcgacgg ctccccgacc cacccgagca acgccggatg
     3661 gcaccggcac tgcgaggccc acgggcacga ggagtacccg cgcaccgatc ccgccgtcat
     3721 ctgcctggtg cacgacggtc gcgaccgcgt cctgctcgcc cgccagccga cgtggccgga
     3781 ggggcggtac tcggtgctcg ccgggttcgt cgaggcgggg gagtccctcg aggcgtgcgt
     3841 gctgcgggag atccacgagg aggtcggcgc caccgttcgt gacgtccgct acctcggcag
     3901 ccaggcctgg ccgttcccgc ggtcgctcat ggtcggcttc cacgccgagg ccgaccccga
     3961 cgtcccgttg gtgcccgccg acggcgagat cgccgaggcg atgtgggtga ccagagccga
     4021 gctccgagca gccctcgagc acggtgactg ggccgccgac gggcagcagg atgcgcgtcc
     4081 gctgctgctg cccggccggg tgtcgatcgc gcgctcgatg ctggagtcct gggccgcgct
     4141 cgactgaccg cacacaccag ctggccaccg tgcacacgtc atgacgtgtg cacggtggcc
     4201 aggaggtggg tgcggtgcgc ctacgccgcc aggtcggcga ccttctcggc gacgaccgcg
     4261 aacggcgggt tcgtcatcgc ggtaccgtcg gcgaacacga cagtggggac cgtctggttg
     4321 ccgccgttga cgctctcgac gaagcgcgct gcgtcgggat cgcgctcgat gtcgacctcg
     4381 ctgtaggcga tgcctgcctg gtccatctgc agcttcagcc gacggcagta accgcaccag
     4441 atcgtcgagt acatcgtcaa atcagccata tcgggttcaa ccaccggcgc ccgtactgtc
     4501 ttcccctggt gtcaggatgg tcgggtgacc gctcgcctcg tccccgacgt tccggaatcc
     4561 ggcctcgacg acgagcagct tgccgcggtc acggctccgc gcggccccgt gtgcgtgctc
     4621 gcgggggcgg gcaccggcaa gacccgcacg atcacccgcc gcatcgccca tctcgtccgc
     4681 acgggccatg ttgcgccggg tcaggtcctc gccgtcacgt tcacggctcg cgcggcgggc
     4741 gagatgcgca cgaggctgcg gacgctcggg gtggcaggcg ttcaggcccg cacgttccac
     4801 gccgccgcgc tgcggcagtt gcgctacttc tggccacgcg ttgtcggcgg tgaccagtgg
     4861 cagctgctcg agggcaagct gcggatcgtc gggcaggcgg ccacacgggc gaaggccggc
     4921 acggacgcag cgtcgctgcg cgatctcgcc ggcgagatcg agtgggccaa ggcaagcctc
     4981 gtcacaccgg aggggtacgc ggcggcggtc gcgaagctgc gccgggacat ccccgcaccc
     5041 gcagaacagg tgtccgcggt ctacgcgggt tacgaggcgc tcaagaaccg cgcggagctg
     5101 ctcgacttcg atgacctgct gctgcacacc gcggcggcgc tggaggagca cggcgcggtc
     5161 gcggaggagt tccgcgaccg ctaccgctgc ttcgtcgtcg acgagtacca ggacgtcacg
     5221 cccctgcagc agcgcgtgct cgacgcctgg ctcggcgacc gcgacgatct cacggtcgtc
     5281 ggcgacgcca accagacgat ctacacgttc gccggcgcca cgccgcgcca tctgctcgac
     5341 ttcccacgcc gcttcccgga ggcggtcgtc gtgcgcctgc aacgtgacta ccggtccacc
     5401 ccgcaggtcg tgtctgcggc caacacgctg atcagttccg cccgcggacg ggtcgcgggc
     5461 acccggctgc agctggtcgg gcagctcccg ccgggccggg agcccgactt cgccgagcac
     5521 gacgacgagc ccgccgaggc agcagccgtg gctgcgcaga tcaagcggct ggtcgccgag
     5581 ggcaccccgg cgagcgagat cgctgttctt ttccggatca atgcgcagtc cgaggtctac
     5641 gagcaggcgc tcaccgacgt gggcgtgccc taccaggtcc gtgggggcga gcgcttcttc
     5701 tcccggcccg aggtgcggcg tgccatgacg gtcctgcgcg ccgccgcact gcggacgccc
     5761 gatcacgaca ccgcggtggt cgacaccgtc cgcgccgtgc tggctcccgt cggactcacc
     5821 ccggagccgc ccggcggcgc cgcgcagcgg gcccagtggg agtcgctgct cgcgatcgtc
     5881 gagctcgccg aggagctcgt ggcggtcgag cccgatgccg acctgcgccg gttcgccgcc
     5941 gagctggaca ccagggccga cgccgcgcac ccgcccaccg tccagggcgt cacgctcgcg
     6001 tccctgcacg ctgcgaaggg actggaatgg gacgtcgtgt tcctcgtcgg gctcgtcgac
     6061 ggcacgctgc cgatccagca cgccgacggt gacgatgagg cgatcgagga ggagcgcagg
     6121 ctgctctacg tgggcatcac ccgggcccga cgccggctcg ccctgtcctg ggcgctgtcc
     6181 cgccagccgg gtggcccgcg gcggaggcgg cgcagccgct tcctctacgg gctcgtgccc
     6241 gagcaccacc cggcgtcgcg catcgccctc ggcgaacgcg gcggggccaa gccgcgctgc
     6301 cgggtctgcg gttctccgct cgtgggggtg gatgcgctga agctgcggcg ctgcgg
//
LOCUS       DASRSC010000056         3943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_836301,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000056 DASRSC010000000
VERSION     DASRSC010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_836301"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFE39_02835"
     CDS             <1..235
                     /locus_tag="VFE39_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257565.1"
                     /translation="SDSDSDSDSDSDSGPGPGPGPLVSCPGAYPDPDPNPDPDSGADP
                     DPGSGTHPAACRRLWSGNLRGSRDQCRRRVRVG"
     gene            246..1127
                     /locus_tag="VFE39_02840"
     CDS             246..1127
                     /locus_tag="VFE39_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET6257566.1"
                     /translation="MDEFEGSLGENWSVYDGPGHAGNGRRTPDAVSVQDGILTITGDS
                     AGNTAGMAWNPGQKYGRWEGRVRAPASDESYNALLLLWPDAENFPVGGEIDFMEMMDH
                     TRQTTNIFLHYGENNDQVEGEVRIDATQWHNWAVEWTPTHVAAFVDGREWWRTTDVDI
                     LPPGPMHLCIQLDWFPDGGDVQESYMYVDWVKQYSLDMGEVVDDVERTVDEVGDAGRA
                     VDGIGDVGRVVEEVRDAGRVVGGIGDVAGAAVNDLRDGPAPAPARGVIRSLTEGTPDP
                     VPPPRAPADRPVPAPGG"
     gene            complement(1154..1708)
                     /locus_tag="VFE39_02845"
     CDS             complement(1154..1708)
                     /locus_tag="VFE39_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET6257567.1"
                     /translation="MKRTGPLPRIAVALRRERERHNISLAELARRAGIAKSTLSQLEA
                     GAGNPSVETLWALAVALDVPFSRLVDPPDAPVQVLRAGEGITIRSEHAPFTGTLLAAC
                     PPGARRDLHLITAEPGPARAAQAHIPGTTEHMLVTTGRWRAGPVGAEVDLDVGDYARF
                     PGDRPHGYEALVPGSAAVLVMEYV"
     gene            1763..2449
                     /locus_tag="VFE39_02850"
     CDS             1763..2449
                     /locus_tag="VFE39_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET6257568.1"
                     /translation="MRSLHRTIDRDDLRDALALAAAVGVVGASFGALAAAAGVPMSLA
                     MALSLLVFAGGSQFLAVAVLAAGGSPIAAVAAGLLINVRHVPFGLAVGGNIADRWSTR
                     LLGAHIMIDEGVAFSRARGSGRRARVAYWMCGTLLFVFWNVGTVVGVLAGSSVPDTDT
                     FGVDAAFPAALLALLLPALRRADARRVGLAAAAVALLATPLLPAGLPVLAGLLGLFAA
                     RLPADARRDP"
     gene            2446..2754
                     /locus_tag="VFE39_02855"
     CDS             2446..2754
                     /locus_tag="VFE39_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET6257569.1"
                     /translation="MNWPAVLALAAGTYLLRVTGIVLRDRVRLPGRVERYVDLGATAL
                     LVALVATAALTDGTGFAGWARPAGVAVGALAAWRKVPFVLVVVLAAGTTSGLRYLGVP
                     "
     gene            complement(2779..>3943)
                     /locus_tag="VFE39_02860"
     CDS             complement(2779..>3943)
                     /locus_tag="VFE39_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HET6257570.1"
                     /translation="RTSADAYVGRVSLVRVFSGTLRPESTVHVSGHHVFIPAQRREEP
                     DGGGPDGHDADERLAHIYAPLGATLREVDACVAGDICALTKLGTAETGDTVSDTEHPL
                     LLSCWDLPQPLLPVAVVARTRGDEDALVKTLGKIVAADPTLRLERNQETHQTVLWCMG
                     EAHADVVLSRLRSGGADVDTEPVRVPMRETIAAPARVTGRHVKQSGGHGQYAVCHVEF
                     EPLSRGAGFEFVSKVVGGAVPTQFIPSVEKGIRAQLERGLTEARHPAVDLRATLVDGK
                     AHSVDSSDAAFQTAGALALREAATVCGTILLEPFDEVTVRVPDEHLGAVLGDLSGRRG
                     RVLGTDIAGPGRTLVRAEVPCAELRRYAVDVRAMTSGAATFTRRFARYDTAPT"
BASE COUNT          513 a         1404 c         1446 g          580 t
ORIGIN      
        1 gtccgactcc gactccgact ccgactccga ctccgactcc ggccccggcc ccggccccgg
       61 cccccttgtt tcctgccccg gtgcctaccc cgaccccgac cccaaccccg accccgattc
      121 cggtgccgac cccgaccccg gctccggtac ccaccccgcc gcctgccgac gactgtggtc
      181 cgggaacctc cgcgggtcac gagaccagtg ccgccgccgc gttcgggtgg ggtgagccga
      241 accgggtgga cgagttcgag gggagcctcg gcgagaactg gagcgtctac gacggcccgg
      301 ggcacgcagg aaacgggagg cgtacaccgg acgcggtcag cgtgcaggac ggcatcctga
      361 ccatcaccgg cgactccgcg ggcaacacgg ccggaatggc ctggaacccg ggtcagaagt
      421 acggccgctg ggagggcagg gtcagggctc cggcttcgga cgagtcgtac aacgcgctgc
      481 tgctgctctg gcccgacgcg gagaacttcc cagtgggtgg ggagatcgac ttcatggaga
      541 tgatggacca cacccgccag acgaccaaca tcttcctgca ctacggagag aacaacgacc
      601 aggtcgaggg cgaggtcagg atcgacgcga cccaatggca caactgggcc gtcgagtgga
      661 caccgacgca tgtggccgcg ttcgtcgacg gccgggagtg gtggcggacc acggacgtgg
      721 acatcctgcc gcccggcccg atgcacctgt gcatccagct cgactggttc ccggacggtg
      781 gcgacgtgca ggagtcctac atgtacgtcg actgggtcaa gcagtactcc ctcgacatgg
      841 gggaggtggt cgatgacgtc gagcgcaccg tcgacgaggt cggggacgcc ggccgcgccg
      901 tcgacgggat cggggatgtc ggccgggtcg tcgaggaggt cagggatgcc ggacgcgtcg
      961 tcggcgggat cggggacgtc gcgggcgcag cggtgaacga cctccgggac ggcccagcac
     1021 ccgccccggc ccggggcgtg atccgctcgc tcacggaggg caccccggat ccggttccgc
     1081 cccctcgcgc accggccgac cggccggtgc ccgcaccggg tggctgagcg aggccaccgc
     1141 tgggccgtcg ctgctacacg tactccatca ccagcaccgc cgcggagccg ggcacgaggg
     1201 cttcgtaccc gtgcggtcgg tcgcccggga agcgggcgta gtcgcccacg tcgaggtcga
     1261 cctccgcccc gaccgggccg gcgcgccacc gtcccgtcgt cacgagcatg tgttcggtgg
     1321 tgccggggat gtgggcctgg gccgcgcggg ccggaccggg ctcggccgtg atgaggtgca
     1381 ggtcgcgccg ggccccgggc gggcacgcgg cgagcaacgt gcccgtgaac ggggcgtgct
     1441 cggaccggat ggtgatgccc tcgccggcgc gcagcacttg gacgggcgca tccggggggt
     1501 cgaccaggcg actgaacggc acatcgagcg ccaccgcgag ggcccacagg gtctcgacgc
     1561 tggggttgcc ggcgcccgcc tcgagctggg agagcgtcga cttcgcgatg ccggcgcgtc
     1621 gggcgagctc ggcgagggag atgttgtgac gctctcgctc cctgcggagc gcgaccgcga
     1681 tcctcggtaa cggccccgtt cgtttcatcg gactcctcgt tcgacttgac gaacacttcg
     1741 gtgtccgttc actctagagg acatgcgttc gctacatcga acgatagacc gggacgacct
     1801 ccgtgacgcc ctcgctctcg cggccgcggt cggcgtggtc ggggcgtcct tcggtgcact
     1861 ggccgcggcg gccggcgtgc cgatgtcact cgcgatggcg ctgtcactgc tggtgttcgc
     1921 cggcggctcg cagttcctcg ccgtcgccgt cctggccgcg ggggggagcc caatcgccgc
     1981 cgtggcagcc ggcctgttga tcaacgttcg acacgtgccg ttcgggctgg ccgtcggcgg
     2041 caacatcgcc gaccgctgga gcacccggct gctcggcgcc cacatcatga tcgacgaggg
     2101 agtggcgttc tcccgggcac ggggcagtgg caggcgggcc cgcgtcgcat actggatgtg
     2161 cggcacgctg ctgttcgtct tctggaacgt cggaacggtc gtcggggtgc tggccggctc
     2221 ttcggtcccc gacacggaca ccttcggagt ggacgccgcg ttccccgccg ccctgctggc
     2281 gctgctgttg ccggcgctgc gtcgggccga cgcccggcgg gtgggtctgg cggcagcggc
     2341 ggtcgcgctg ctggcgacac cattgctgcc ggccgggttg ccggtgctcg ccggcctgct
     2401 cgggctgttc gcggcgaggt tgcctgccga cgcccggagg gatccgtgaa ctggccggcg
     2461 gtgctggctc tcgcggcagg cacctacctg ctgcgggtga cgggcatcgt cctgcgggac
     2521 cgcgttcgtc ttcccggccg ggtggagcgc tacgtcgacc tcggggcgac cgcgctgctg
     2581 gttgcgctgg tggccaccgc cgcgctcacc gatggcaccg gtttcgccgg ttgggcacgg
     2641 cccgcgggcg tcgccgtcgg ggcgctcgcg gcctggcgaa aagtcccgtt cgtgctggtc
     2701 gtcgtcctgg ccgcgggcac cacctcgggg ctgcggtacc tcggcgtgcc ctgacctgca
     2761 tctaccgacg aggcagggtc aggtcggcgc tgtgtcgtaa cgtgcgaagc ggcgggtgaa
     2821 cgtcgctgcg ccactcgtca tcgcccgcac gtcgacggcg tagcgccgca gctcggcgca
     2881 cggcacctcg gcccgcacca gcgtccgccc cgggcccgcg atgtcggtgc cgagcacccg
     2941 cccacgccgc ccagacaggt caccgagcac ggcccccagg tgctcgtccg gcacccgcac
     3001 cgtcacctcg tcgaacggtt cgagcaggat cgtgccgcag accgtcgccg cctcccgcag
     3061 cgccagcgca cccgcggtct ggaacgcggc gtcggaggag tcgacgctgt gtgccttgcc
     3121 gtccacgagc gtggcacgca ggtcgacggc aggatggcgc gcctcggtga ggccgcgttc
     3181 gagctgggcg cggatgcctt tctccacgct cgggatgaat tgcgtgggca ctgcgcctcc
     3241 caccaccttc gagacgaact cgaagcccgc tccgcgcgac aggggctcga actcgacgtg
     3301 gcacaccgcg tattggccgt ggccgcccga ttgcttgacg tgcctgccgg tgacgcgcgc
     3361 cggggcggcg atcgtctcgc gcatcggcac ccgtaccggc tcggtgtcga cgtcggcccc
     3421 gccggaacgt agccgcgaca gcacgacgtc ggcgtgggcc tcacccatgc accacagcac
     3481 cgtctggtgg gtttcctggt tgcgctccag gcggagtgtg gggtcggcgg cgacgatctt
     3541 gccgagtgtc ttgaccaggg cgtcctcgtc accccgcgtc cgggcgacca ccgcgacggg
     3601 cagtagcggc tggggcaggt cccagcagga gagcagcaac gggtgctcgg tgtcggacac
     3661 ggtgtcgccg gtctcggcgg tcccgagctt ggtcagggcg cagatatcgc ccgccacgca
     3721 cgcgtccacc tcgcgcaacg tcgcgccgag cggggcgtag atgtgggcga ggcgctcgtc
     3781 ggcgtcgtgg ccgtcgggcc ctccaccgtc gggctcctca cgccgctggg ccgggatgaa
     3841 cacgtggtgc ccgctgacgt ggacggtcga ctccgggcgc agtgtcccgg agaacacccg
     3901 caccagcgag acgcgcccca cgtaggcgtc ggccgaggtg cgc
//
LOCUS       DASRSC010000057        18126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5094787,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000057 DASRSC010000000
VERSION     DASRSC010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 18126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18126
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5094787"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /gene="mihF"
                     /locus_tag="VFE39_02865"
     CDS             <1..292
                     /gene="mihF"
                     /locus_tag="VFE39_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005449622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HET6257571.1"
                     /translation="PEQRAEALKKAAAARTARKELRDSIARGEQTIPAVLDRAKSDTI
                     VGKTKVADLLKSLPGYGPAKVSALLEQTGIDASRRAAGLGDRQRQALLDALK"
     gene            436..1299
                     /locus_tag="VFE39_02870"
     CDS             436..1299
                     /locus_tag="VFE39_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6257572.1"
                     /translation="MERYVDVAPGVRLWAEDIAPTGPRPGEPLLLVMGAGSSGLVWPD
                     ALLGLLAERHRVIRYDHRDTGRSTAAFDEHPYGITDLAGDALAVLDAFDVPRAHVVGM
                     SMGGMLVQLMLLDHPERLASATLFCTGALPLPGAPELPGPDQALLRLWGELDDPRDLE
                     GELRWRVEHWRLLNGSGTLFDDHEFRALEERVIAHTGRHDPVVAHAHMDTSGLERGAE
                     LAAVTVPTLVIEAPEDPAYPPPNSGHLVRTIGHARMVRIPGMGHTINRTVTSPLAAAI
                     LTQTTHTAAPA"
     gene            1382..1618
                     /locus_tag="VFE39_02875"
     CDS             1382..1618
                     /locus_tag="VFE39_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257573.1"
                     /translation="MGIEDPDADVVEQLTPIEDDDDAVEDPSGLPLEADPADVAEQRQ
                     VVPVEDPSELPLEADPADVAEQRQVVPEYDDEPR"
     gene            1979..2872
                     /locus_tag="VFE39_02880"
     CDS             1979..2872
                     /locus_tag="VFE39_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6257574.1"
                     /translation="MKVISIINHKGGVGKTTLTANLGAGLAARGQKVLLVDLDAQASL
                     TVSFFTQSEWTADLLPSKTIKHWFDGLGTAEAMQDPAPLVSSPPRVHDKLARSRGTLG
                     LIAAHRDLSDVETSLAAELLLGNPDRFGELHGRLRDGLAHPALADYDTVLIDCAPNFG
                     LIAKNAVAASDLLLIPTKPDYLSTNGIDSLGLKIRSFIEEYNEYSPVPVQRPAAAVVF
                     TMVQNHDGAPIEAQRSVISRIVAREVPTFSTTIRDSKSVYGPAPEHGVPVILGGTGRA
                     QTRELRDMVSELSGRLAGSAA"
     gene            2869..3297
                     /locus_tag="VFE39_02885"
     CDS             2869..3297
                     /locus_tag="VFE39_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257575.1"
                     /translation="MTAPTGSAPLTAALQQATAVLETLTQQQLDDLVAGRGQLVFRPA
                     PHTANARSTTPTRRPRVEVDITADVAEINRLGTPGEVDDYLHQHDSRFTVPVLRQIAR
                     ALGPTVATTGRTKAEIRRDIVAGTAGFRTRSAAMSGGAWS"
     gene            complement(3302..3709)
                     /locus_tag="VFE39_02890"
     CDS             complement(3302..3709)
                     /locus_tag="VFE39_02890"
                     /inference="COORDINATES: protein motif:HMM:NF019533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HET6257576.1"
                     /translation="MDGLPGTPVEMGRSVIEVVRRTDTHALRMRNPAVGVVCFELDGQ
                     EHELVALPGKTGGLSLHSRDATSGDSTYPGGRIVRTTEPAPDGRLTLDLNRVVNLPCG
                     FTDFATCLLPPAENVLPVAVEAGERTPLRPDHR"
     gene            complement(3825..4205)
                     /locus_tag="VFE39_02895"
     CDS             complement(3825..4205)
                     /locus_tag="VFE39_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257577.1"
                     /translation="MDDWVSAALTADVTAAAAAVQVAAVTEAGRQADALLGEPPVPGS
                     VEWHAEEGTDIPLRRERAWQLLQLRIGLGAGLDPLPVLIGLRRHGSTWEEIARAAGIT
                     RQSAHERWAARVADVLDRYGDAGG"
     gene            4296..5015
                     /locus_tag="VFE39_02900"
     CDS             4296..5015
                     /locus_tag="VFE39_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6257578.1"
                     /translation="MSAPPTETDKQRRLRARLDELIASMSPGEPLPAERDLARDLGVA
                     RMTLRRAVDGMVADALLVRRQGAGTFVAPAKVAHRLSANSFSADMRARGMRPGSCTLS
                     SSRAPAGVMLAALLEVSPGTPVLHVRRLRLADDEPMAVEDLHVPGDLVPGLTGAELED
                     RSYYELLAERYGHRIASGTQTAEAALTGAEDAAALGVQPGAPAFCFERTCRVDGGRVA
                     EFVRSIYRGDRYRIMVDIFPS"
     gene            5156..6439
                     /locus_tag="VFE39_02905"
     CDS             5156..6439
                     /locus_tag="VFE39_02905"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET6257579.1"
                     /translation="MRRSAGALLAISALALAGCGGAGQGTTDAGSGGDRTLSVWIMEG
                     TNPDARPFFDELSTAFRTQTGATLDVQFVPWASAHDKFVTSIAGGTTPDVAEVGTTWT
                     GEFGEAGALVDLAPRVRDAGLSNGLVTGLTEAGTVGDALYGMPWYAGVRSIVYRTDVF
                     TEAGVTPPTTWDELVTAGQKIKAARPDLVPFPVAGDNEYGVYPFVWGAGGQIATENGG
                     KWTSALDSPQARQGIQFYADLALKHGLSTPAAATWKETDLQDAFTKGQAAMIISGSWT
                     PKAILEAAPELQGKIGAFPIPGPSGGLAGSFLGGSHLSIFEGSKNQDLAWQLVQLMST
                     GDFAAKWGAESSFFPGTTALLQQAAQSSDPLVAPFAKQMVDAGRSVPVTPLYGQVQGK
                     KTIPAMMSAILTGTGVDQATSTAAAEMNQIFAGGA"
     gene            6436..7419
                     /locus_tag="VFE39_02910"
     CDS             6436..7419
                     /locus_tag="VFE39_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6257580.1"
                     /translation="MTDGVTTVRERTRSGRGAATAGPRPGRSRRSGSRAARPWLLLAP
                     ALLVMGGLLLYPLLRVVVISLQDFGLRQLVSGRTSWIGFDNYAELLGDPDLWRTVLPN
                     TVVFAVVAVALTVVVGTLVALLLARLRPLTRGIVTTAIMLAWAVPAVTGTYVWVFLFT
                     PGDGIAVQALDALGLTDAATANPFASRISFYAVATLNVVHHGFPFVALTVLAGLLTVP
                     QEVTEAAIVDGAGAWRRFWSVTFPMLRPVFAVVTVLSTIWDFKVFTQIYLMPGGDGVN
                     RGVLNLGTWSYVESFAQNRYGLGAAIAVLLTVLLLIITVGYLRLLFKEDEL"
     gene            7416..8231
                     /locus_tag="VFE39_02915"
     CDS             7416..8231
                     /locus_tag="VFE39_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6257581.1"
                     /translation="MRASSLGKGLGVAGLLLFTLFPVYLMVVTAFNGRANAGSRSLWP
                     EDWTFRNFTFVLGEGGFGRYLSNSLLVALGTVLAGALLALLAAVAVARFRFRFRTSVL
                     VMILIIQMVPVEALVIPLFIQARSLGVLDSLFGLVVVYLAFSLPFAIWTLRGFVAAVP
                     VELEEAAYLDGASWGRMFWSVLLPLVAPGLVATSVFSFITAWNEFVFALTFMADQDNY
                     TVAVGLRRFFGQYGTDWGALMAGSTLITLPVMVFFVIAQKRLTDGLVAGAVKG"
     gene            8228..9643
                     /locus_tag="VFE39_02920"
     CDS             8228..9643
                     /locus_tag="VFE39_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET6257582.1"
                     /translation="MSSDARLVDAVLLPGFTGEAVPDWLARAVDGGLAGVCVFAPNVG
                     PGFDALTRGLHDRRDGMLVASDEEGGAVTRLEHREGSSWPGAAALGRLDDTGVTEAVA
                     AGMGRQCRAAGVDLALAPVADVNAEPENPVIGIRSFGATPDLVARHTAAFVRGLQGAG
                     VAAAAKHFPGHGSTTVDSHRALPVVDADLTTLRHRDLPPFVAAVEAGVRCVLTAHVLF
                     PALDDRPATMSQALLGLLRRELGFDGVVISDALDMHAISRGIGRGPGAVAALAAGVDL
                     VCTGNPGFPERYDDEAGYLEVRRALLTALTDGTLARSRVADAGRRVAELADWVSRTTR
                     VDARSADAAELGLQVVRRVVAVAGDVRAGRGPHVLDLTGPENVAAGRRREPWLVAALR
                     RRDPSVTVGGRDVPGDGPLVVVATGPSPALDAVRAARPHAVVVHVGLPLAWTPPAPSL
                     TVWGDGRAQAEVAAEMLTGIS"
     gene            9730..11337
                     /locus_tag="VFE39_02925"
     CDS             9730..11337
                     /locus_tag="VFE39_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6257583.1"
                     /translation="MSDEQREQGRDASASPDGPRTDQHPVQSYGPGHEPTLPSMTGPT
                     QGPGQAPGQEPAAPYSAGPAQAPGQEPGGPYAAGPQAPGQEPAGPYAGPRHAPGQAPP
                     GNPYPDTAAYPQPHGAVPYGGPPMPVAGPPRKPRVMNGIVAAAVIAGLVGGGAGFGGA
                     YALLGHDPVAAILTSASSSAAPVAATPGSVAAAAQTAMPSTVDIRVTLAQGTAEGSGV
                     ILTADGDVLTNNHVVAGSTGQILVTLADGSQHPATVVGTAPSYDLAVIKLQGVSGLTP
                     ATLGQSGSLQVGQQVVAIGSPQGLTGTVTSGIVSAFNRTVQVQGENGSGVVYNGLQTD
                     APINPGNSGGPLVNLQGQVIGINSAIATASSQNDGSVGLGFAIPVDQAKRVAQELIST
                     GTATKPVLGVQGNASSSTAGEGAPIAAVQAGSPAAQAGLTAGDVITKVDNNQVQNFAD
                     LIARIGSYAPGQQVTLTIGNGGATRTVNVTLGSAPDKAATPSGQGQGGQGQGQGQGQG
                     QGQNPFGGGNPFSGGGNPFGGGNPFGN"
     gene            complement(11400..12551)
                     /locus_tag="VFE39_02930"
     CDS             complement(11400..12551)
                     /locus_tag="VFE39_02930"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET6257584.1"
                     /translation="MLDDGQPATGPLAGVVVADFSRILAGPYATMLLADLGATVIKVE
                     GPGTGDDTRTWVPPTADDGTATYYLSINRGKRSIVLDLADPADLDVAQRLAARADVMI
                     ENFKPGGLRRFGLDHETVAARNPRIVYCSISGFGSAAGAALPGYDLLVQATSGLMSVT
                     GAADGPPYRAGVAVFDVITGLHAAVAIMAALHHRERTGTGQKVETNLLSAALSGLVNQ
                     TSAVLTGGVVPRRLGNAHLSLFPYEPLPTGDGELIVIAANDGQFRRLAIAIGAPELLD
                     DPRFGSMGDRNAHRDELRSLLLARLATRSAQEWFDILAPAGVPCGPINTIDEGLALAE
                     RLGLDPVVKPGGVPSVRNPVTYSVTPPSYDRPPPALGADGPEIRAWLHE"
     gene            complement(12606..14081)
                     /locus_tag="VFE39_02935"
     CDS             complement(12606..14081)
                     /locus_tag="VFE39_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6257585.1"
                     /translation="MTSTGTTSTGTTSDGAGAAPRPAPSRWVALAVLCAGMLMVILDG
                     TIVSVALPAIQADLGFTATGLAWTVNAYLVPLGGLLLLAGRLGDLLGRRRMLVAGLAL
                     FVVASLLCGLAVGAGMLVVARFLQGVAAAMASAVVLGMIVGLFPEPGERARAIGVYAF
                     VGAAGASIGTLLGGVLTETAGWRWIFFVNVPIGVAAVLLSLRVIERDRPVGGAGRPDL
                     LGGVLVTAGLGLAVLTIVDTSGPEVRLGLAAPALALLVGFVVRQSRAADPLLRLGIFR
                     SRAVSGGNLVQLLMVAGMLGFQFTAALHLQGALGYAPAQTGLALLPIPVTIAVVSLAG
                     SGRLIARFGPRPVLLVGEVLLVAGLLLLTRPPAGSYLVDVLPSMLVLGIGAGLSLPAV
                     TTVMMSDATPADAGLASGLANTSQQVGGALGIAVLAALATARTDAAKATGVPLQEALA
                     GGYHLAFAAGACALALAALLTLTVLRPRARVTPPTVIGLSA"
     gene            complement(14157..14660)
                     /locus_tag="VFE39_02940"
     CDS             complement(14157..14660)
                     /locus_tag="VFE39_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6257586.1"
                     /translation="MAVRSDDEIRGDLSYLFDKASQALAVRMTAALAELDITPRGYCV
                     LSKAMPGELTQGELAEIALLDKTTMVVTLDHLEGAGLARRTPSRTDRRARIVETTEEG
                     DRVVTTARTIIDGVYDEVLGVLPDAQRSVLLDALVALVGAGGPLSTVEPTPNAPRRRS
                     QRSSRSV"
     gene            complement(14737..15474)
                     /locus_tag="VFE39_02945"
     CDS             complement(14737..15474)
                     /locus_tag="VFE39_02945"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HET6257587.1"
                     /translation="MNETRRSDQLAQRDPFVLVHGACHGGWCWVRVARLLRAAGHEVF
                     TPTLTGFGERAHLLTAEVGPATLIQDVVAVLEHEELRDVVLVGHSFGALVALGVADRL
                     PHRLRRLVLLDGVVVEPGEPAFSGLPSEAVEARTAAAKASPNGLTYPPPPAAAFGLSD
                     PDDMAWVDRLLTPQPLRSYSEPFGLRAPLGNGLPISYVCCTDPPYPVIQPAHAIVRRE
                     GWEWRELATGHDAMITAPDATAAELLR"
     gene            complement(15500..17641)
                     /locus_tag="VFE39_02950"
     CDS             complement(15500..17641)
                     /locus_tag="VFE39_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET6257588.1"
                     /translation="MSSRPGQGAGRLLRGGRVAARVLLVWALSAGALRLLDLWLPGFA
                     MADWWQPTVCALLFGVLSAVVWPLVLRVALPIAVFSLGFGSFLLLGVGVLAISFVVPG
                     VVIADFETAVVVAVAVSVLATLGSSLLALDQDELFFRRAARRSARRAAGGDGEMPPGV
                     LFLQVDGLGMDTLRRAVRDGDMPTFARWLDAGSHRLTEWHTDWSSQTGASVCGILHGD
                     NSDIPGFRWYEKDRDHVMACAHPEDAAEIERRHSDGRGLLSIDGAGHGNLFTGDAPHV
                     SLTMSALPVLTGAARRRRRGSSDGVGAGYYAYFANPANTLRTLAGALVDVVREISAST
                     RQRRADVRPRVNRGGFYPFARSATTVIVRDVVVSAIIEDMLAGRAAVYADFLGYDEVA
                     HHSGVERFDALAVLRSIDQQIGRLHRATQLAPRRYHIVALSDHGQTQGWAFTDRFGES
                     IEALVGRLCGAPQPEPRRGVNSDARRPAEGWQVGAALAEASTGSGPIARRLRARVEGA
                     GSVQDRPRTESGAPGAVTRVAPGVVVVASGHVAMVSFTEHEGRVELETIEREFPELLP
                     ALVDHAGVGFMLVRSAEFGPVVLGRDGLHRLAAGVVVGEDPLIPYGPHAADLVRRVDG
                     FAHSPDIMINSRYDAARDEASPFEPHVGSHGGMGGPQSRGFLLHPSDLPAPGEIVGAE
                     ALHRVLRGWLTHVGQPEPAVDAQTSGTVAAG"
     gene            complement(17638..>18126)
                     /locus_tag="VFE39_02955"
     CDS             complement(17638..>18126)
                     /locus_tag="VFE39_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET6257589.1"
                     /translation="SLPLVVLLAALGALLGDLVTFGVARAGSGAALRWVARGQNPERL
                     ASARRQFATRGWQLVVIGRLVPAGRIPVLLAAGALGYPWRRLVPAAAGACLLWAIAYA
                     ALGIVSGGLFDDPLIATLLAAVLVLVIGAVASLVSSRRRRRKAAAGAATAVADDPRSA
                     AP"
BASE COUNT         2625 a         6660 c         6432 g         2409 t
ORIGIN      
        1 tcccgagcag cgcgccgagg ccctcaagaa ggcagccgct gcccgcaccg ctcgcaagga
       61 actgcgcgac tcgatcgccc gcggggagca gaccatcccc gcggtgctcg accgcgccaa
      121 gtccgacacc atcgtcggca agacgaaggt ggccgacctg ctcaagagcc ttccgggcta
      181 cggcccggcc aaggtctccg cgctgctgga gcagaccggt atcgacgcct cccggcgcgc
      241 cgccgggctc ggcgaccgcc agcgccaggc gctgctcgac gccctgaagt aggccctcct
      301 gcgggccaga agcacgcctg acggcacacc cgttgcaccg gtcccggcgc tcgcgccggg
      361 accgacagcg ggtgatccgg cgcgtgttgc ggtaccggtt cgacacgcgc caccgccgac
      421 cggcaggctg gccccgtgga acgctacgtc gatgtcgccc cgggagtccg gctctgggca
      481 gaggacatcg ctcccaccgg gccacgcccc ggtgaaccgc tgctgctggt gatgggtgcc
      541 gggtcgtccg gcctggtgtg gccggacgcg ctgctcggcc tgctcgcgga acggcatcgg
      601 gtcatccggt acgaccatcg cgacaccggc cgctccaccg ccgccttcga cgagcatccg
      661 tacgggatca ccgatctcgc gggcgacgcg ctcgccgtcc tggacgcgtt cgacgttccc
      721 agggcgcacg tcgtgggcat gtcgatgggc ggcatgctcg tgcagctgat gctgctcgac
      781 catcccgagc gcctcgcctc ggccaccctc ttctgcacgg gcgcccttcc gcttcccggc
      841 gcgcccgagc tccccggccc cgaccaggcg ctcctgcgtt tgtggggcga gctggacgac
      901 ccgcgcgacc tcgagggtga gctccggtgg cgggtggagc actggcgtct cctcaacgga
      961 tccgggaccc tcttcgacga ccacgagttc cgcgccctcg aggagcgggt gatcgcacac
     1021 acgggtcggc acgatccggt cgtcgcccat gcccacatgg acaccagcgg cctcgagcgg
     1081 ggcgccgagc tcgccgccgt gacggtgccg acgctcgtga tcgaggcgcc ggaggaccct
     1141 gcctacccgc cgccgaactc gggtcacctg gtccgcacca tcggccatgc tcggatggtg
     1201 cggatccccg ggatgggcca caccatcaac cgcacggtga cctcgccgct cgccgcggca
     1261 atcctgacgc agacgaccca cacggccgct ccggcctgat gcctctggcg acgatgtgta
     1321 cgccactcgg tggtcgggca ctcccgcgcg cccgcacgac aaccgcatgg aaggtggacg
     1381 gatgggcatc gaggatcccg acgcggacgt ggtcgagcag ctgaccccga tcgaggacga
     1441 cgacgacgcg gttgaagacc cgagcggact cccgctggag gccgacccgg ccgacgtcgc
     1501 cgagcagcgc caggtggtcc cggtggaaga cccgagcgaa ctcccgctgg aggccgaccc
     1561 cgccgacgtc gccgagcagc gccaggtggt cccggagtac gacgacgagc cgaggtgacc
     1621 cccggcgaga cggcccccgg ttaggacccc gacgatccca cctcgtggac gcagcgtcat
     1681 ccgaccgggt gacttttcgc aattgccgct cccgcgccgc accggacatc gcacccctgc
     1741 accgcgcgtc ggaccactcg tcggggcgag gcggtgggtg cgccgcgggc gcctgggcac
     1801 cttctccgtc cgcttcccgc acctcggcga gcccggagac gacggctggc acagcggggg
     1861 cgacctcgag cacctgtcgt cgcccaccga acgggcgaca cgacacggag tgacctaagg
     1921 gcaagccccg tcgagccata tggcatcgtt cccgccgcgt gacgcgggga ggagaacggt
     1981 gaaggtcatt tcgatcatca accacaaagg cggcgtgggg aagaccaccc tcaccgccaa
     2041 cctcggagcc ggactggcgg ccaggggtca aaaggtctta ctcgtcgatc tcgatgcgca
     2101 ggccagcctg actgtctcgt tcttcacaca gagcgagtgg acggcggatc tgctcccgag
     2161 caaaaccatc aagcactggt tcgacggcct tggcacggcg gaggcgatgc aggaccccgc
     2221 tccgctcgtc tcgtcgccac cgcgggttca cgacaagctc gcccgcagcc gcggcacgct
     2281 cggcctcatc gccgcgcacc gcgacctctc cgacgtcgag acgtcgctcg ccgccgaact
     2341 cctcctgggc aacccggacc gcttcgggga gctgcacggg cggctgcggg acggcctcgc
     2401 ccacccggcg ttggccgact acgacaccgt cctgatcgac tgcgcgccca acttcggact
     2461 catcgcgaag aacgccgtag cggcgagcga cctgctgctg atccccacca agccggacta
     2521 tttgtcgacc aacgggatcg actcgctcgg actgaagatc aggtcgttca tcgaggagta
     2581 caacgagtac tcgccggtgc cggtccagcg gccggccgcc gcggtcgtct tcacgatggt
     2641 ccagaaccac gacggagcgc ccatcgaggc ccagcgcagc gtcatcagcc gaatcgtggc
     2701 gcgggaggtg ccgacgttct ccacgacgat cagggacagc aagtctgtgt acgggccggc
     2761 cccggagcac ggggtgccgg tgatcctcgg cggcacgggc cgggcccaga cccgggagct
     2821 gcgggacatg gtgtccgagc tctccggcag gctcgcgggg agcgccgcgt gaccgctccg
     2881 acagggtccg ctccgctcac cgccgcgctg cagcaggcca cggccgtgct cgagacactc
     2941 acccagcagc agctggacga cctcgtcgcc gggcgcggcc agctcgtctt ccggcccgct
     3001 ccccacaccg ccaacgcgcg ctccaccact ccgacgcgcc ggccccgcgt cgaggtcgac
     3061 atcacggccg acgtcgcgga gatcaaccgg ctgggcaccc cgggcgaggt ggacgactac
     3121 ctgcaccagc acgactcccg gttcaccgtc ccggtgctac gccagattgc cagggcactc
     3181 ggcccgacgg tcgcgaccac cggacgcacc aaggccgaga tccgccgtga catcgtcgcg
     3241 ggcaccgccg ggttccgcac ccgctccgcc gcgatgtcgg gcggggcctg gtcgtagcgc
     3301 cctagcggtg gtcgggccgc agcggggtcc gttcccctgc ctcgacggcc accgggagca
     3361 cgttctcagc gggcggcagc aggcaggtgg cgaagtcggt gaacccgcac ggcaggttga
     3421 ccactcggtt gaggtcgagg gtcagcctgc cgtccggtgc aggttcggtg gtccgcacga
     3481 tccggccgcc cgggtaggtg gagtcgccgg acgtggcgtc gcgggagtgc agcgacagcc
     3541 caccggtctt gcccggcagc gcgacgagct cgtgctcctg tccgtcgagc tcgaaacaca
     3601 ccacgccgac cgcggggttg cgcatccgga gggcgtgcgt gtcggtgcgc cggacgacct
     3661 cgatgaccga gcggcccatc tcgaccggcg tccccggcag tccgtccaca agtgcagggt
     3721 gaccgtcccg tcgatccgac gcggctcgcg aaccgtccgg gcccgtgcgc ggatacgagt
     3781 gccggggccc tcgtatccgc gcacgagggc cgaaggcctg acgatcaacc gccggcgtcg
     3841 ccgtagcggt cgagcacgtc ggcgacccgg gcggcccagc gctcgtgcgc ggactgccgg
     3901 gtgatgcctg cggcgcgagc gatctcctcc cacgtggagc cgtgccggcg cagtccgatc
     3961 agcaccggca gcgggtccaa ccccgccccg agcccgatcc gcagctgcag cagctgccac
     4021 gcccgttcgc ggcgaagggg tatgtcggtg ccctcctccg cgtgccactc cacgctgccg
     4081 gggacgggtg gctcgccgag cagtgcgtcg gcctgccgcc ccgcttccgt cacggcggcc
     4141 acctgcacag cagcggctgc ggcggtcaca tccgccgtca gggccgcgct cacccagtcg
     4201 tccatcctgt caggatcatc ctgacagatg gtttcgtcaa cccggtcgag gtgtggcgat
     4261 ggccgcagcc gggaccattg gtgtaccaat gggctatgag cgcgccgcca acggagaccg
     4321 acaagcagcg gcgcttgcgt gcgcgtctcg acgagctgat cgcgtccatg tcgccgggcg
     4381 agccgctgcc ggccgaacgt gacctcgccc gcgacctcgg ggtcgcccgg atgacgctgc
     4441 gccgcgccgt ggacgggatg gtggccgacg cactgctggt ccgacggcag ggtgcgggca
     4501 cgttcgtcgc accggcgaag gtggcccacc ggctctcggc gaactccttc tccgccgaca
     4561 tgcgcgcccg cggaatgcgg ccgggcagct gcaccctctc gagcagccgg gcacctgccg
     4621 gggtcatgct ggcggccctg ctggaggtgt caccgggcac tccggtgctg cacgtccgcc
     4681 ggctgcgact ggccgacgac gagccgatgg ccgtggagga cctgcacgtc cccggcgatc
     4741 tcgtcccggg cctgacgggt gcggagctgg aggaccggtc ctactacgaa ctgctggccg
     4801 agcggtacgg ccaccggatc gcctccggca cgcagaccgc ggaggccgcg ctgaccggag
     4861 ccgaggacgc tgccgccctc ggcgtccagc ccggggctcc ggccttctgc ttcgagcgca
     4921 cctgccgcgt ggacggcggc cgcgtggccg agttcgtccg ctcgatctac cgcggcgacc
     4981 ggtaccggat catggtcgac atcttcccgt cctgatccct cgtacaccct tgacgccgcc
     5041 agggtgacga gcgctacagt tccggacatt tagacctctg gtctcgatga ccggtctgct
     5101 ctggcgtgcg tgtcggaagg ggaaccacat gtggtggacg tccggacccc ggagggtgcg
     5161 gcgcagcgcc ggcgccctcc tcgccatctc ggcgctggcg ctcgcgggct gcggcggcgc
     5221 aggccagggc accaccgacg ccggcagcgg cggcgaccgc accctctcgg tgtggatcat
     5281 ggagggcacc aacccggacg cccgcccgtt cttcgacgag ctctcgacgg ccttccgcac
     5341 ccagaccggc gccaccctcg acgtgcagtt cgtgccgtgg gcctcggcgc acgacaagtt
     5401 cgtcacctcg atcgccgggg gtaccacacc ggacgtcgcc gaggtgggca ccacctggac
     5461 cggggagttc ggcgaggcgg gcgctctggt cgacctcgcc ccgcgcgtgc gggacgcggg
     5521 cctgtccaac ggcctggtca cggggctcac ggaggcgggc acggtgggcg acgccctcta
     5581 cggcatgccg tggtacgcgg gggtccggtc gatcgtctac cgcacggacg tcttcaccga
     5641 ggccggcgtc accccgccca ccacgtggga cgagctcgtc acggcagggc agaagatcaa
     5701 ggccgcgcgg ccggacctgg tcccgttccc ggtcgcgggc gacaacgagt acggcgtgta
     5761 ccccttcgtc tggggggcag gcgggcagat cgccaccgag aacggcggca agtggacgag
     5821 cgccctcgac tcgccgcagg cgcgccaggg catccagttc tacgccgacc tcgccctgaa
     5881 gcacgggctg tccacgcctg cggcggccac ctggaaggag accgacctgc aagacgcgtt
     5941 caccaaaggt caggcggcga tgatcatctc cggcagctgg acgccgaagg cgattctcga
     6001 ggcggctccg gagctgcagg gcaagatcgg cgcgttcccg atccccggcc cgtcgggcgg
     6061 cctcgcaggg tcgttcctcg gcggctcgca cctgtcgatc ttcgaaggca gcaagaacca
     6121 ggacctcgcc tggcagctgg tgcagctgat gagcaccggt gacttcgccg cgaagtgggg
     6181 cgcggagtcg agcttcttcc ccggcaccac ggcgctgctg cagcaggccg cgcagtcgtc
     6241 cgacccgctc gtggcgccgt tcgccaagca gatggtcgac gccgggcgga gcgtgccggt
     6301 gacgccgctc tacgggcagg tccagggcaa gaagacgatt ccggcgatga tgagcgcgat
     6361 cctcaccggc acgggcgtgg accaggcgac ctcgacagct gccgcggaga tgaaccagat
     6421 cttcgcgggc ggggcgtgac cgacggcgtg acaacggtcc gggagcgcac ccgatcgggt
     6481 cgcggggcgg ccaccgccgg cccgcgcccc ggccgctccc gccgcagcgg gagcagggcc
     6541 gcccggccat ggctgctgct cgcgcccgcg ctgctcgtca tgggcgggct gctgctctac
     6601 ccgctgctcc gggtcgtcgt gatctcactg caggacttcg gcctgcggca gctcgtctcg
     6661 ggccgcacga gctggatcgg cttcgacaac tacgccgagc tgctcggcga ccccgatctg
     6721 tggcgcacgg tcctgcccaa caccgtggtc ttcgccgtgg tggcggtggc gctgacggtg
     6781 gtggtcggaa cgctggtcgc gctgctgctg gcccggctgc gccccctgac ccgcggaatc
     6841 gtcacgacgg cgatcatgct ggcgtgggcg gtcccggctg tgaccggcac ctacgtctgg
     6901 gtcttcctct tcacaccggg ggacgggatc gcggtccagg ccctcgacgc gctcggcctc
     6961 accgacgcgg ccaccgccaa cccgttcgcg tcgcgcatct ccttctacgc cgtggccacg
     7021 ctcaacgtgg tgcaccacgg tttcccgttc gtggcgctca ccgtgctcgc cgggctgctc
     7081 accgtgccgc aggaggtcac ggaagccgcg atcgtggacg gcgccggcgc gtggcggcgg
     7141 ttctggtcag tcacatttcc catgctgcgg ccggtgttcg ccgtggtgac ggtgctctcc
     7201 acgatctggg acttcaaggt gttcacccag atctacctga tgcccggcgg cgacggcgtc
     7261 aaccgcggcg tgctcaacct cggcacctgg tcctacgtcg agtcgttcgc ccagaaccgc
     7321 tacgggctcg gcgcggcgat cgccgtgctg ctcaccgtgc tgctactgat catcacggtc
     7381 ggctacctgc ggctgctgtt caaggaggac gagctgtgag ggcgagcagt ctgggcaagg
     7441 gcctcggcgt tgcgggcctg ctgctcttca cgctgttccc ggtgtacctg atggtcgtca
     7501 cggcgttcaa cgggcgggcc aacgccggtt cgcgctccct gtggcccgag gactggacgt
     7561 tccgcaactt cacgttcgtg ctcggcgagg gagggttcgg ccggtacctc tccaactcgc
     7621 tgctcgtcgc gctcggcacc gtgctggccg gtgccctgct cgcgcttctg gcggccgtgg
     7681 ccgtcgcccg gttccgcttc cggttccgca cctcggtact ggtgatgatc ctgatcatcc
     7741 agatggtgcc ggtggaggcg ctggtcatcc cgctgttcat ccaggcgaga agtctcggcg
     7801 tgctcgacag cctgttcggg ctcgtcgtgg tctacctggc gttctccctg ccgttcgcga
     7861 tctggacgct gcgcggtttc gtcgccgcgg tgcccgtgga gctggaggag gcggcctatc
     7921 tcgacggcgc gtcgtggggg cgcatgttct ggtcggtgct gctgccgctg gtggcgcccg
     7981 gcctggtggc caccagcgtg ttctccttca tcacggcgtg gaacgagttc gtcttcgcgc
     8041 tgacgttcat ggccgaccag gacaactaca ccgtcgcggt cgggctgcgc cgcttcttcg
     8101 ggcagtacgg caccgactgg ggcgcgctga tggccgggtc cacgctgatc acgcttccgg
     8161 tcatggtctt cttcgtgatc gcgcagaagc ggctgaccga cgggctcgtg gccggggcgg
     8221 tgaaggggtg agctcggacg cgcggctggt cgatgcggtg ctgctccccg ggttcaccgg
     8281 ggaggccgtg ccggactggc tcgcccgcgc cgtcgacgga ggcctcgcgg gggtgtgcgt
     8341 gttcgcgccg aacgtgggac ccggcttcga cgcgctcacc cgcgggttgc acgaccggcg
     8401 cgacggcatg ctcgtcgcgt ccgacgagga gggcggcgct gtcacccggc tcgagcaccg
     8461 cgaggggtcg tcgtggcccg gtgccgccgc cctgggcagg ctcgacgaca ccggcgtgac
     8521 cgaagccgtg gcggccggaa tgggtcggca gtgccgagcg gccggggtgg acctcgcgct
     8581 cgcgccggtg gccgacgtca acgccgagcc ggagaacccg gtgatcggta tccggtcctt
     8641 cggcgccact ccggacctgg tggcccggca cacggcggcg ttcgtgcgcg ggctgcaggg
     8701 tgcgggcgtg gcagcggcgg cgaagcactt ccccggccac gggtcgacca ccgtcgactc
     8761 ccaccgggcg ctgccggtcg tcgacgccga cctcacgaca ctgcgccatc gcgacctgcc
     8821 accgttcgtc gccgccgtcg aggcaggcgt gcgctgcgtg ctcaccgcac acgtgctgtt
     8881 ccccgcgctg gacgaccggc cggccacgat gagccaggcg ctgctcgggc tgctgcgccg
     8941 cgagctgggc ttcgacggcg tggtgatcag cgacgccctc gacatgcacg cgatctcccg
     9001 cggcatcggg cgcggcccgg gagcagtggc agcgctcgcc gcgggcgtcg acctggtgtg
     9061 caccggtaac ccgggcttcc cggagcgcta cgacgacgaa gccggctacc tcgaggtgcg
     9121 ccgcgcgctg ctcaccgccc tgaccgacgg cacgctggcc aggagccggg tggcggacgc
     9181 cggccggcgc gtggcggagc tggcggactg ggtgtcccgc accactcggg tcgatgcgcg
     9241 ctccgctgac gctgcggagc tgggcctgca ggtcgtccgg cgggtggtcg cggtggccgg
     9301 ggacgtacgg gccggacggg ggccgcacgt gctcgacctc accggcccgg agaacgtggc
     9361 ggcgggccgg cgccgcgagc cgtggctggt cgcggccctg cggcggcgtg acccgagcgt
     9421 cacggtggga gggcgggacg tcccgggaga cgggccgctc gtcgtcgtcg cgaccggccc
     9481 gtcgcctgcc ctggatgcgg tgcgggccgc caggccgcac gcggtggtcg tccacgtcgg
     9541 gttgccgctg gcatggacgc ccccagcgcc gtcgctgacg gtgtggggcg acgggcgcgc
     9601 gcaggccgag gttgcggcgg agatgcttac ggggatctcc tgacccgccg cgcaccttgt
     9661 ccgaaatcac aggtcgtttc ccagggactc ctcagcaagc tcctagtttt cgggcgcatc
     9721 gtcgttgtca tgagcgacga gcagagggag cagggccggg acgcgagcgc ctcgcccgat
     9781 ggcccccgca ccgaccagca ccccgttcag tcgtacgggc ctggccacga gccgacattg
     9841 ccctccatga cgggtccgac gcaggggccg ggacaggcgc ccggccagga accggcggcg
     9901 ccctacagcg caggcccggc acaggcacca ggccaggaac cgggggggcc ctacgccgca
     9961 ggcccgcagg cgcccggcca ggaaccggcg gggccctacg caggcccgag gcacgcgccc
    10021 ggacaggcgc cccccggcaa cccgtacccg gacacagccg cgtacccgca gccgcacggt
    10081 gccgtcccct acggtggccc gcccatgccg gtcgccgggc cgccgcgcaa accgcgcgtg
    10141 atgaacggca tcgtcgcggc ggcggtgatc gccgggctcg tcggcggcgg cgccggattc
    10201 ggcggagcgt acgcgctgct cgggcacgac cccgtggcag cgatactgac ctccgcctcg
    10261 tcctcggcgg cgcccgtggc cgcgacgccc ggctcggtgg cggcagccgc ccagacggcc
    10321 atgccgagca ccgtcgacat ccgcgtcacc ttggcccagg gcaccgccga gggcagcggc
    10381 gtgatcctca ccgccgacgg cgacgtgctc accaacaacc acgtcgttgc gggctccacg
    10441 ggtcagatcc tggtgaccct cgccgacggg agccagcacc ccgccaccgt cgtcggcacc
    10501 gcaccgagct acgacctcgc ggtgatcaag ctgcagggcg tctccgggct cacccccgcg
    10561 accctcggcc agagcgggag cttgcaggtg ggccagcagg tcgtggctat cgggtcgcca
    10621 cagggtctca ccggcacggt caccagtggc atcgtcagtg cgttcaaccg cacagtgcag
    10681 gtccagggcg agaacggctc cggggtcgtc tacaacggcc tgcagaccga cgcgcccatc
    10741 aaccccggca actcgggcgg cccgctcgtg aacctccaag gccaggtcat cggcatcaac
    10801 tccgcgatcg cgacggccag ctcgcagaac gacggcagtg tcgggctggg cttcgcgatc
    10861 cccgtcgacc aggccaagcg ggtggcgcag gagctcatca gcaccggcac ggccaccaag
    10921 ccggtgctcg gtgtgcaggg caacgcctcg tcctccaccg cgggcgaggg tgctccgatc
    10981 gccgccgtgc aggccggctc accggccgca caggccgggc tgaccgccgg tgatgtcatc
    11041 accaaggtgg acaacaacca ggtgcagaac ttcgccgacc tcatcgcccg gatcggctcc
    11101 tacgctcctg gccagcaggt gacgctgacg atcggcaacg gcggcgccac tcgcacggtc
    11161 aacgtgacgc tgggcagcgc gcccgacaag gcggccacgc cgagcggcca gggccagggg
    11221 ggccagggcc agggccaggg ccagggccag ggccagggcc agaatccgtt cggcggtggc
    11281 aaccccttca gcgggggcgg taacccgttc gggggcggca accccttcgg caactgatcc
    11341 ggtcgggggc acgacggcac tctcgtccag gtgtgtggac gagagtgccg tcgtgagtgc
    11401 tactcgtgga gccaggcgcg gatctcgggg ccgtccgccc cgagcgccgg cggcgggcga
    11461 tcgtaggacg gtggggtcac ggagtacgtc accgggttgc gcacgctcgg gacgccaccg
    11521 ggcttcacca ccgggtcgag cccgagccgc tcggcgaggg ccagtccctc gtcgatggtg
    11581 ttgatcgggc cacacggaac ccctgcaggc gcgaggatgt cgaaccactc ctgcgccgac
    11641 ctggtggcga ggcgggccag cagcagcgag cgcagctcgt cgcggtgggc gttgcggtcg
    11701 cccatcgatc cgaaccgcgg gtcgtcgagc agctcaggcg cgccgatcgc gatggcgagc
    11761 cgccggaact gcccgtcgtt ggccgcgatg acgatcaatt cgccgtcgcc ggtcggcagc
    11821 ggctcgtagg ggaacaggct caggtgcgcg ttgccgagcc gccgggggac gacgccgccg
    11881 gtcagcaccg ccgacgtctg gttgaccagc ccggacaacg cggcggacag caggttcgtc
    11941 tccaccttct ggcccgtgcc ggtgcgctcc cggtggtgca gcgccgccat gatcgcgacg
    12001 gcggcgtgca gcccggtgat cacgtcgaag acggccaccc cggcgcggta gggcggcccg
    12061 tcggcagctc cggtgacgct catcaggccc gacgtcgcct gcacgagcag gtcgtagccc
    12121 ggcagtgcgg ctccggcggc ggagccgaag ccgctgatcg agcagtagac gatccgcggg
    12181 ttcctggccg ccaccgtctc gtggtcgagc ccgaaccggc gcagcccacc cggcttgaag
    12241 ttctcgatca tgacgtcggc gcgcgcggcg agccgctgcg cgacatcgag gtcggcggga
    12301 tccgcgaggt ccaggacgat cgaacgcttg ccccgattga tcgacaggta gtaggtggcc
    12361 gtgccgtcgt cggcagtcgg aggcacccac gtgcgggtgt cgtcgccggt accgggtccc
    12421 tccaccttga tcaccgtggc gccgaggtcg gcgagcagca tcgtggcgta gggcccggcg
    12481 aggatgcggg agaaatcggc gacgaccaca ccggccaggg gtccagtggc cggctggccg
    12541 tcatcgagca cgcaaggacc ctatgtcagc ccctcgccga cgcaggatca gacgtgccca
    12601 cgctctcagg cggagagtcc gatgaccgtc ggcggcgtca cccgggcgcg ggggcgcagc
    12661 acggtcaggg tgagcagcgc cgccagcgcc agtgcgcagg cgcccgccgc gaaggccagg
    12721 tggtagccgc cggccagggc ttcctgcagg ggcacgcccg tagctttcgc ggcgtcggta
    12781 cgggcggtcg cgagcgcggc cagcaccgcg atccccagcg ccccacccac ctgctggctc
    12841 gtgttggcaa gtccggacgc cagtccggca tcggccggtg tggcgtcgga catcatcacg
    12901 gtcgtgacgg cgggcaggga gagcccggcg cctatgccga gcacgagcat cgacggcagc
    12961 acgtccacca gatagctccc agccgggggg cgggtcagga gcagcaggcc cgccacgagg
    13021 agcacctcgc cgaccaggag cacggggcgc ggcccgaacc gggcgatcag ccgccccgac
    13081 ccagcgagcg agacgaccgc gatcgtcacc gggatcggca ggagcgcgag gccggtctgt
    13141 gccggcgcgt agcccagcgc gccttgcagg tggagcgccg cggtgaactg gaagcccagc
    13201 atcccggcca ccatgagcag ctgcaccagg ttgccgcccg acacggcgcg cgaccggaag
    13261 atgcccagcc gcaggagcgg gtcagccgcg cgcgactggc ggacgacgaa cccgacgagc
    13321 agcgccagcg ccggcgcggc gaggccgagc cggacctccg gccccgacgt gtcgacgatc
    13381 gtgagcaccg cgagccccag gcccgccgtg acgagcacgc ccccgagcag gtcgggccgc
    13441 ccggcgccac ccaccggccg gtcacgctcg atcacccgca gggagagcag caccgctgca
    13501 accccgatcg ggacgttgac gaagaagatc caacgccagc ccgcggtctc ggtgagcacc
    13561 ccgccgagca gcgtgccgat ggacgccccg gcggcaccga cgaacgcgta gacgccgatc
    13621 gcccgcgccc gctcgcccgg ctcggggaag agcccgacga tcatgccgag aaccaccgcc
    13681 gacgccatcg ccgcggccac gccctgcagg aaccgggcca cgacgagcat tccggcgccg
    13741 acggcgaggc cgcatagcag cgaggccacg acgaagagcg cgaggcccgc caccagcatg
    13801 cggcgcctgc ccaagaggtc gccgagcctg ccggccagca ggagcagccc gccgagcggg
    13861 acgaggtagg cgttgaccgt ccaggccagt ccggtggccg tgaagcccag gtcggcctgg
    13921 atcgccggaa gcgcgacgct gacgatcgtg ccgtcgagaa tgaccatcag catgcccgcg
    13981 cagagcacag cgagcgcgac ccatcgcgag ggtgcagggc ggggggcagc accggcgccg
    14041 tccgatgtcg tgccggtcga tgtcgtgccg gtcgatgtca tggctgtgac ctcctggttg
    14101 tgccgaccag gagagaccgt agcagatagt tctattctag acgatcctgt taagtatcaa
    14161 actgatcggg acgaccgctg cgatcgacga cggggggcgt tgggcgtggg ctcgactgtc
    14221 gagagcgggc ctccagcgcc gaccagggcg accagggcgt ccagcaggac gctccgctgc
    14281 gcgtcgggca ggacccccag cacctcgtca tagacgccgt cgatgatcgt gcgcgccgtg
    14341 gtgaccaccc gatcgccctc ctcggtggtc tccacgatcc gggccctgcg gtccgtgcgc
    14401 gacggcgtgc ggcgagcgag ccccgcgcct tcgagatggt ccagcgtgac gaccatggtg
    14461 gtcttgtcga gcaacgcgat ctcggccagc tcgccctgcg tcagctcacc cggcatcgcc
    14521 ttggacagca cgcaatagcc ccgcggcgtg atgtcgagct cggccagtgc ggcggtcatc
    14581 cgcaccgcca gcgcctggct ggccttgtcg aacaggtagc tcaggtcacc gcggatctca
    14641 tcgtcgctgc gtacggccat gagggccatg gtacagatga tctgtgttca gatcatcccg
    14701 caacagatcg atgtcggagc gttctcgacg cagcggtcag cgcagcagct cggcagccgt
    14761 cgcgtccggc gcggtgatca tcgcgtcgtg cccggtggcg agctcgcgcc actcccaacc
    14821 ctcgcgccgc acgatcgcgt gggcgggctg gatcaccggg tatggcgggt cggtacagca
    14881 gacgtagctg atcggcaacc cgttgcccag cggcgcccgc agcccgaacg gctcgctgta
    14941 ggagcgcagc ggctgcgggg tgagcagccg gtcgacccag gccatgtcgt cggggtcgct
    15001 caggccgaac gcggcagcgg gcgggggcgg gtaggtcaac ccgttcgggg acgccttcgc
    15061 cgcggccgtg cgggcctcca ccgcctcgga gggaagcccg ctgaacgccg gctcgcccgg
    15121 ttccaccacc accccgtcga gcaggacgag gcggcgcagc cggtgtggca gccggtcggc
    15181 gacgccgagg gcgacgagcg cgccgaagct gtgtcccacc agcacgacgt cgcgcagctc
    15241 ctcgtgctcc agcacggcga cgacgtcctg tatcagggtg gccggcccga cctcggctgt
    15301 cagcagatgc gcgcgttcgc cgaagcccgt gagcgtcggc gtgaagacct cgtgccctgc
    15361 cgcgcgcagt agccgcgcaa cccgcaccca gcaccagccg ccgtggcacg cgccgtgcac
    15421 cagcacgaag ggatcgcgct gtgccagctg gtcgctcctc ctcgtctcgt tcatggaccg
    15481 agtctggcag gaaatgccct caccccgccg cgacggttcc cgaggtctgc gcgtcgacgg
    15541 cgggctccgg ctgcccgacg tgggtcagcc agccacgcag cacccggtgc agcgcctcgg
    15601 cgcccacgat ctcccctggt gcggggaggt ccgaggggtg gaggaggaaa cctctcgact
    15661 gggggcctcc catccccccg tgtgagccga cgtgcggctc gaaaggcgat gcctcgtccc
    15721 tggccgcgtc gtacctactg ttgatcatga tgtccgggga gtgcgcgaaa ccgtccaccc
    15781 gccggacgag gtcggcggcg tgcggcccgt aggggatcag cgggtcctcg ccgaccacca
    15841 ccccggccgc caaccggtgc agcccgtcgc ggccgagcac gacgggcccg aactccgcgc
    15901 tgcgcaccag catgaagccg acgcctgcgt ggtcgacgag tgcgggcagc agctcgggga
    15961 actcgcgctc gatcgtctcc agctcgaccc ggccctcgtg ctcggtgaac gagaccatcg
    16021 ccacgtgccc cgacgcgacc acgactacgc cgggagccac ccgggtgacc gcccctggcg
    16081 caccgctctc ggtgcgcggg cggtcctgca ccgagcctgc cccctcgacc cgcgcccgca
    16141 gcctgcgcgc gatcgggccg ctcccggtcg aggcctcggc cagcgcggca cccacctgcc
    16201 acccctcggc gggccgtcgc gcatcggagt tgacgccacg gcgcggctcc ggttgcggtg
    16261 ccccgcacag tcgcccgacc agcgcctcga tggactcccc gaaccgatcg gtgaacgccc
    16321 agccctgcgt ctgaccgtgg tcggacagcg ccacgatgtg gtagcggcga ggcgcaagct
    16381 gagtggcccg gtgcagcctc ccgatctgct ggtcgatcga gcgcagtacc gcaagggcgt
    16441 cgaaccgctc gacgccggag tggtgggcca cctcgtcgta cccgaggaag tcggcgtaga
    16501 ccgccgcccg accggccagc atgtcctcga tgatcgccga gacgacgacg tcgcggacga
    16561 tcacggtggt ggcactgcgg gcgaacgggt agaacccgcc ccggttcacc cgcggccgca
    16621 cgtcggcgcg ccgctgccgc gtggaggcgg agatctcccg gacgacgtcg acgagcgccc
    16681 cggccagggt gcgcagcgtg ttggccgggt tcgcgaagta ggcgtagtag ccggcgccga
    16741 ccccgtccga ggagccgcgg cgacgtctgc gcgccgcgcc ggtcagcacc ggcaacgcgc
    16801 tcatcgtcag gctgacgtgg ggcgcgtcgc cggtgaagag gttgccgtgg cctgcgccgt
    16861 cgatgctgag cagcccgcgc ccgtcggagt ggcgccgctc gatctcggct gcatcctcgg
    16921 ggtgcgcaca ggccatgacg tggtcgcggt ccttctcgta ccagcggaac ccggggatgt
    16981 cggagttgtc gccgtgcagg atgccgcaga cgctcgctcc ggtctgcgag ctccagtctg
    17041 tgtgccactc ggtgaggcga tgactcccgg cgtcgagcca gcgggcgaac gtcggcatgt
    17101 ccccgtcacg cacggcccgc cgcagagtgt ccatgcccag cccgtcgacc tgcaggaaca
    17161 gcacgcccgg cggcatctcc ccgtcgccgc cggcagctcg gcgggcgctg cggcgcgccg
    17221 cgcgccggaa gaacagctcg tcctggtcga gcgccaacag gctggaaccg agcgtggcga
    17281 gcacagagac cgcgaccgcc acgaccaccg ctgtctcgaa gtcggcgatc accactcccg
    17341 gcacgacgaa ggagatcgcc agcaccccga cgccgagcag caggaacgac ccgaacccca
    17401 gtgagaacac cgcgatcggc agcgcgaccc gcaggaccaa cggccagacg acagcgctga
    17461 gcacgccgaa cagcagggcg cacaccgtcg gctgccacca gtccgccatc gcgaaacccg
    17521 ggagccacag gtccagcagc cgcagcgcac cggccgacaa cgcccacacc aggagtaccc
    17581 gcgcagcgac ccggccgccc cgcaacaacc gtccggcgcc ctgaccgggt cgggagctca
    17641 cggggctgcg cttcgggggt cgtcggcgac cgcggttgca gctccggccg ccgccttccg
    17701 gcgcctgcgc ctgctgctca ccagcgaggc gacggcaccg atcacgagca cgagcaccgc
    17761 ggcaagcagc gtagcgatca gcggatcgtc gaacaggccg ccgctgacga tccccagcgc
    17821 ggcgtaggcg atcgcccaca gcaggcacgc tcccgccgcg gccggcacca gccgccgcca
    17881 cgggtagccg agcgcgcccg cggccagcag caccgggatc cggcccgccg gcacgagcct
    17941 gccgatcacg accagctgcc aaccgcgggt ggcgaactgc ctgcgggccg acgcgagccg
    18001 ctccgggttc tggccccgcg ccacccaccg cagcgccgca ccgctgcccg ccctggccac
    18061 accgaacgtc accaggtcac cgagcagggc tcccagcgcg gccagcagga cgacgagcgg
    18121 gagcga
//
LOCUS       DASRSC010000058        16703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_183731,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000058 DASRSC010000000
VERSION     DASRSC010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 16703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16703
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183731"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1934)
                     /locus_tag="VFE39_02960"
     CDS             complement(<1..1934)
                     /locus_tag="VFE39_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET6257590.1"
                     /translation="MTSTQTSSATTTATTTAGRIGDSPLRPDGALKVTGEFAYASDLW
                     HDDMVWGVTLRSPHPHARIVAIDIGEALAVPGVSAVLTAADIPGSNAVGLEHLDTPAL
                     ATGVVRYEGEPVALVAADHPETARRAAKKIIVEYAVLPAVTDPRHALDADAPLVHESR
                     RHDGGNVVRKMQVRRGDPAPTAPIVVALDFEVGMQDQAFLGPESGLAVPAEDGGVDLY
                     VATQWLHVDQQQVCRVLGLPPEQVRLHLAGVGGAFGGREDLSMHVHACLLALHTGKPV
                     KMSYSREESFFGHVHRHPAFMRYEFGAEPDGTLVYAKADILFDGGAYASSTGAVVGNG
                     GTLGLGPYRIPNVHVDCAGVYTNNPPCGAMRGFGCVQAAFAHEALMDSLADAVGVDRV
                     EIRARNGMREGDRNITGQLIDSAAPVAELVRIVRDMPLPAERSPNEVDIRELPGGVSN
                     TTHGEGVVRGIGYAVTYKNVGFSEGFDDHSTARVRLEITGGEPVATVHTAAAEVGQGL
                     ITVEQQICRTELGVERVVVHPKDTAVGSAGSTSASRQTYVTGGAVKAACEAVRAAVLT
                     RAAALLERSRESLGLDGDKIVDTSGEVLAGLADVLGEDAVEETVEWRHRPTRAIDPET
                     GQGDAHVQYAFSAHRAVVDV"
     gene            complement(1940..3304)
                     /locus_tag="VFE39_02965"
     CDS             complement(1940..3304)
                     /locus_tag="VFE39_02965"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HET6257591.1"
                     /translation="MTTVSQRALVIEGAHVVTVSGAEFPGGHVVVEDNRITAVAAGPA
                     PHVEGALVVDGRGCLLTPGFVNTHNHLYQWVTRGLAVDSTLFEWLTTLYPVWAGIDEH
                     AVHTAASAALAQLALTGCTTTTDHHYVFPREGGDVFAAEIRAAQDVGLRFHPCRGSMD
                     LGQSDGGLPPDEVVEDRDTVLAASEAAIDRWHDPSPGAMLRVALAPCSPFSVTGELMR
                     DSAELARRRGVMLHTHLAETVDEEAFCRERFGCSPLEYVERFGWTGEDVWFAHAVHLD
                     DEAVKKIGATATGVAHCPSSNARLGAGIARTRDLRAAGARVALGVDGAASNEAGSLLE
                     EVRHALLFARAVGGPQALTVRDALELATLGGASVVGRTDEIGSVEVGKLADIALWRID
                     GLAHADIADPVAALVLGSPPPLQLLLVNGRAVVEGGRVSTVDEDALARECTAVHRTLL
                     EKAG"
     gene            complement(3301..3780)
                     /locus_tag="VFE39_02970"
     CDS             complement(3301..3780)
                     /locus_tag="VFE39_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET6257592.1"
                     /translation="MRITLTVNGTEHDVDDVWEGESLLYALRERMGLPGSKNACEQGE
                     CGSCTVYLDGLPVCSCLVAVGQAAGREVTTVEGLSAEGELHPVQRAFLECGAVQCGFC
                     TPGLIVSTHDLLERNGRPSDPEIREALAGNLCRCTGYEKIMDAVRLAAATTSGEHAS"
     gene            complement(3782..4654)
                     /locus_tag="VFE39_02975"
     CDS             complement(3782..4654)
                     /locus_tag="VFE39_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET6257593.1"
                     /translation="MEFLAPTTWADALAAKAEHPGALPVTGGTDVMVEINFDQRRPPA
                     LLDLTRVPELREWAPDAGMIRLGAAVTYARVIDELGGTLPGLAMAARTIGSPQIRNRG
                     TVGGNLGAASPAGDSHPPLLAADAVVEVASAARGMRRVPVAQFYTGVKRNALAPDELI
                     AAVLIPPPTGPQQFCKIGTRNAMVIAVSAFGLALHPDRKAVGTGIGSAAPTPRRADEA
                     AEFLSGELDAAGLWESRGELPDGLAREFGHGVSAAAAPIDDVRGSAAYRLHSLTVMAR
                     RAVTWAWNDYRKAA"
     gene            4748..6304
                     /locus_tag="VFE39_02980"
     CDS             4748..6304
                     /locus_tag="VFE39_02980"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HET6257594.1"
                     /translation="MLLRDLVGHPGLGLRLLHGDDTVLDRPVRWVYTTDLIDPARYLS
                     GGELVLSGLVWRREPGDSERFVAALARSGAAALAAGAAVFHGIPDDLVEACRHHDVPL
                     IAVPEELSFATVTELVITSLTAARGDRLALTVGRQRRLLSAVAAGLGLDELAAQVSAT
                     TGTTCRVLSTTGREIAAGPQPLPDDVLDRIVAAFLTADQLPATLSSDNYTVFPIGPAA
                     PQRLTSWFVLADGDPAGWDAETNDTVGELAAVAALDRARRNEGLRWGRDIADDAVALV
                     AGGAGNRPETSVRLRQAGLDPAEPLVVVTAAFVGRPELVETARAVLVDVAAHRGPATV
                     GVHDGLAVALLPGPDQDEPSGDLTGAVLRRALRRLVPGLAGLRLTVGVSRPSRPDALS
                     GALQEARHARDVAEQRPGALHVVTGAELTSHVALLATVPDDVRRAFARRVLGPVLDHD
                     VRTGAGLRETLEAFLDCSGSWTRTAERLHLHVNTVRYRIGRVEQLTGRDLGTLTDRVD
                     VFLAIRSQMS"
     gene            6338..6916
                     /locus_tag="VFE39_02985"
     CDS             6338..6916
                     /locus_tag="VFE39_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6257595.1"
                     /translation="MSRGDATREIILDEAGRLARRVGLGGLTIGSLATQTGMSKSGLF
                     AHFGSKESMQLQVLDHAVERFTTEVIRPAIKEPRGEPRLRALFDHWLVWDSTEGGCPL
                     VAATFELDDQPGPVREHLVASQRDWLDTIATVVSSGITEGHFRSDTDPRQFAQDLDGV
                     MLAYHVASRLLADPQAAARARRGLDALLAAAA"
     gene            7033..7551
                     /locus_tag="VFE39_02990"
     CDS             7033..7551
                     /locus_tag="VFE39_02990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6257596.1"
                     /translation="MRPLNRQEAGHVVDAVFAQLSPQSRFLRFHSPVPRLPGPVRAGL
                     VDLDGRSRAAVVAEIPGPDGPEPVGIARLACTGDGTADVAVAVVDAWQRRGVGSRLVA
                     ALAALAEQLGYTKLSGAVLPENVAMLGMVRSVFPAVLSHYDGDVVQVAVRTGTSAWAV
                     TEEDVMADLVRR"
     gene            complement(7623..8489)
                     /gene="pucL"
                     /locus_tag="VFE39_02995"
     CDS             complement(7623..8489)
                     /gene="pucL"
                     /locus_tag="VFE39_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004846 - urate oxidase activity [Evidence
                     IEA];
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate oxidase"
                     /protein_id="HET6257597.1"
                     /translation="MGIVLGGHQYGKAENRLVRIYRDSARHEIRDVTVSTTLRGDFEA
                     AYLHGDQSAVLPTDSQKNAAYAFAKEKGLREIEEYALTLARHFVEDVEPVRGARIAVD
                     ELAWERVQVGGEGHDHTWVRRGPEVRTTVVDVEGGRADVVSGLRDLVLLKSTGSEFAG
                     FLTDRYTTLAETDDRIMATALVAQWRHAAPADGSWDGSVDWAKSWSEVRQTLLEQYAT
                     LHSRALQQTLWEMGRAVLEANPGITEINLQAPNKHHFAVDLAPFGLDNGNEVFFAADR
                     PYGLIEATVTRS"
     gene            complement(8495..8818)
                     /gene="uraH"
                     /locus_tag="VFE39_03000"
     CDS             complement(8495..8818)
                     /gene="uraH"
                     /locus_tag="VFE39_03000"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HET6257598.1"
                     /translation="MTLSTHVLDAALGRPAAGVGVRLTAVPGGEPLAEARTDDDGRVR
                     DLAADLAAGAYRITFDTGGYFAATGQQGFYPEVAVTFSVIDPGEHHHVPLLLSPFAYS
                     TYRGS"
     gene            complement(8815..9297)
                     /gene="uraD"
                     /locus_tag="VFE39_03005"
     CDS             complement(8815..9297)
                     /gene="uraD"
                     /locus_tag="VFE39_03005"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HET6257599.1"
                     /translation="MRLEQFNTLPPHEAERELLTCCAAPRWARTVAARRPYASITALH
                     AAAGAALDDADLDAAMAGHPRIGDRQAGGQSRREQSGVGGAAPDVLAALEEGNRAYEG
                     RFGCVYLVCATGRSAEDLLAILHARLANDPAAERAVALAELAAINRIRLDQLVGQDVP
                     "
     gene            complement(9309..10949)
                     /locus_tag="VFE39_03010"
     CDS             complement(9309..10949)
                     /locus_tag="VFE39_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET6257600.1"
                     /translation="MSGAVVEKSPGESARVSGESRIAVEAERRRTFAVISHPDAGKST
                     LTEALALYAEVIDEAGAVHGKSGRRGVTSDWMEMERKRGISITSAVLQFAYRDCVINL
                     LDTPGHGDFSEDTYRVLAAVDAAVMLLDAAKGLEPQTLKLFDVCRTRGIPVLTFVNKW
                     DRPGREALELLDEVEQTIGLRPMPITWPVGVAGELRGLVETDTGEFTRFTRTAGGATR
                     ATSEVVRAEQIAAEEPEFWPAAQDELALLREIGAGFDEKDFLNGSATPVLFGAALAGV
                     GVSRLLDALVDLAPAPEARPDAEGRPRELDAPTSGLVFKVQAGMDRAHRDRMAFVRIC
                     SGRFERGMVLTHAATGRPFATKYAQAVFGQQRTTVEEAFPGDIVGLVNAGALRPGDTL
                     YASDPVEFPAIPSFAPEYFAVVRSADAGSYKQFRRGIEQLDSEGVIQVLSSDLRGDQA
                     PVLAAVGPLQFEVVTARLESEFRAPVTLETLGYSVTRRTDAVGAELLKGQSEVEVLTR
                     RADGVVLALFSNKWRLETVRRKLDGLVLDPLPAGSSER"
     gene            complement(10980..12644)
                     /locus_tag="VFE39_03015"
     CDS             complement(10980..12644)
                     /locus_tag="VFE39_03015"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HET6257601.1"
                     /translation="MTVLIDARGIAPGGRVSADVCVIGTGPAGAVVATELAHRGVDVV
                     VVEGGGQRFGPRQADTYRGEAVDLHDPLEAVRQKRLGGTSAVWGGRCAPLDELDLGPR
                     EWMHTAGWPITAEEVGPYYRRAQRHLQAGQYEYSAARAGFPAATVPPSETLDVDSTWR
                     WSPPTSFAGRMRVLAKASNVRLLVEATVVRLERDPVSGAIVEAVVAPSPGVEITVGAR
                     TYVLAAGGLESARILLASNRQSPAGVGNEHDQVGRHYMTHPVGEVGTVVLSDLGRNLA
                     LGYVPTRDGVYARRMLALTPAAQARHEVGNLKAALWFPDPTDPSHGDGLLSTFSLTYY
                     ALNRARLGFKSAGTHARYAGTSGLGRHVGNVARDWRRVGGFAASWAAPRVTGRRILPS
                     FMPLDGATCRLRFDAEQSPDPANRVILSHERDQLGQRRLQVHYGVSGDDRGRIRRSMT
                     LIGEEFDRLGYGTVVMDDAARIDDMAFTDGTHQMGLLRMAHSPRDGVVDAECRVHGSA
                     NLFVAGSAVFPTAGAVGPTLTIAALACRVADRIAADRTVREPLVAS"
     gene            12713..13843
                     /locus_tag="VFE39_03020"
     CDS             12713..13843
                     /locus_tag="VFE39_03020"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HET6257602.1"
                     /translation="MRFREMAAAVRVLARNDLARYNGSGTSETGRFEDELGRMLGVEH
                     ALAVNSGTSALIGALVGAGIGPGDEVLVPAFTWVATAAAPLAVGAVPVLVEIDESLTM
                     DPADLARKITPHTRAVIPVHMLNLVCDMDSIMKVAAEHDLVVIEDTCQAIGVRHHGRR
                     VGSIGHVGAFSFNQHKNITCGEGGAVVTNDGRIHARAAMHHDVGSYERPGWIETEALF
                     PGVNHRMPELSAAILRPQLAALDAQLDRRRQRRRMVLDRVASGSWTGITTSPHHDETD
                     AVGLALTFEDPAQARAFGELRGARRLIDSGRHVYINWQSVLAKRSFHPLFDPHAWAHR
                     EVDYGPDSCPATLEILARSCAVELAPEVPTPAFRFLTRGMSR"
     gene            complement(13869..14273)
                     /locus_tag="VFE39_03025"
     CDS             complement(13869..14273)
                     /locus_tag="VFE39_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6257603.1"
                     /translation="MQIGEVATRVGLSLRTIRHWDEVGLVVPSKRSAGGFRLYTEADI
                     DRLVLVKTLKPLDFSLEQMRELLATMDGLENDTGADSELTAELMGRLAVFRAAADSRV
                     EALRAQVQGLEMLSRELRRLAGENRRPSAGKG"
     gene            complement(14427..14888)
                     /locus_tag="VFE39_03030"
     CDS             complement(14427..14888)
                     /locus_tag="VFE39_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257604.1"
                     /translation="MHRYLLVANQTLGSDELVDLVNERMAAGPAEFWLVVPATPVKDL
                     ALTAMAIPMPVMGGVPTIPAPPAEARRLAQAKLDAAIKRFTSAGATIGGEVCDPDPMR
                     AVEDAVTSREFDEIIVSTLPARLSRWLHQDLPGRLGRKFQLPVTHVAAKDV"
     gene            15037..15354
                     /locus_tag="VFE39_03035"
     CDS             15037..15354
                     /locus_tag="VFE39_03035"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HET6257605.1"
                     /translation="MSEPDRPIFLVVTIKPRLDRMAEAEAQLQSMRRNTLTEPGCVFM
                     HLVQPQDDPETWVMLEMFRSRAAWDEHMRQPYNTEGNIILQDLLREPSDLRLFDEKQP
                     RTT"
     gene            15526..>16703
                     /gene="fucP"
                     /locus_tag="VFE39_03040"
     CDS             15526..>16703
                     /gene="fucP"
                     /locus_tag="VFE39_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HET6257606.1"
                     /translation="MARGSVTRVQPREEGARKPGLVYPGLTIPFILVVTCFAAWGSAA
                     NLTDVLVGVFRHIFAMSNFQSALVQFAYYGAYFSLAIPAALINRRYGYKAGVLTGLGL
                     ATLGGLLFIPASLLLTYGFFLIALFVLASGLSILETSANPFVIAMGPEASATQRLNLA
                     QSFNPVGANIGVLLGAVLILPQITPESAKVSMTAEQLRQVQEQDLSLVLKPYTGIATV
                     LLLIWILIALRKISVPEEHSEFGLDAPGRSGILGRLWHNRHYRYGVAAQFLNVGAQVC
                     TWTFTIQYAQDVVGVSTANSGWYLQASLILFLISRFVMTYLLGIFRPTKLLFAMGALG
                     VVLALVAVLSPNMVGLIAVVAISVSLSLMFPTIYGVALRGLGKDTKFGAAGLVMAILG
                     G"
BASE COUNT         2388 a         6051 c         5816 g         2448 t
ORIGIN      
        1 tccacgtcga cgacggcgcg atgcgccgag aacgcgtact gcacgtgggc gtccccctgg
       61 cccgtttccg ggtcgatcgc ccgtgtcggg cggtgccgcc actcgacggt ctcctccacc
      121 gcgtcctcgc cgagcacgtc ggccaggcct gcgagcacct cgccgctcgt gtcgacgatc
      181 ttgtcgccgt ccagtccgag actctcgcgg gaccgctcga gcagggccgc ggcccgggtc
      241 agcacggcgg cgcggacggc ctcgcatgcc gccttgaccg cgccgccggt cacgtaggtc
      301 tgccgggacg ccgaggtcga gccggcggaa ccgacggccg tgtccttggg gtgcaccacc
      361 acccgctcga cgccgagctc ggtacgacag atctgctgct cgacggtgat caggccctgc
      421 ccgacctccg ccgcagcggt gtgcaccgtg gcgacgggct cgccaccggt gatctccagc
      481 cgtacccggg cggtggagtg gtcgtcgaag ccctcggaga acccgacgtt cttgtaggtg
      541 accgcgtagc cgatcccgcg tacgacaccc tcgccgtggg tcgtgttcga gacaccgccg
      601 ggcagctccc ggatgtcgac ctcgttgggg ctgcgttcgg ccggaagcgg catgtcccgc
      661 acgatccgca ccagctcggc gaccggcgcc gccgagtcga tcagctgccc ggtgatgttc
      721 cggtcgccct cccgcatgcc gttgcgggcg cggatctcca cccggtcgac tcccacggcg
      781 tcggccagtg agtccatcag cgcctcgtgt gcgaacgcgg cctgtacgca gccgaagccg
      841 cgcatcgccc cacacggcgg gttgttggtg tagacgccgg cacagtcgac gtgcacgttg
      901 gggatccggt acggaccgag gcccagcgtg ccgccgttgc cgacgaccgc cccggtcgac
      961 gaggcgtagg cgccgccgtc gaagaggatg tcggccttcg cgtagacgag ggtgccgtcg
     1021 ggctcggcgc cgaactcgta gcgcatgaac gccgggtgcc ggtgcacatg cccgaagaac
     1081 gactcctcac gggagtagct catcttcacc ggcttcccgg tgtgcagggc gagcaggcag
     1141 gcgtggacgt gcatcgacag gtcctcccgg ccgccgaacg cgcctccgac cccggcgaga
     1201 tgcaggcgca cctgctcggg cggcagcccg agcacgcggc acacctgctg ctggtcgaca
     1261 tgcagccact gggtggcgac gtacaggtcg acgccaccgt cctcggcggg gacggcgaga
     1321 ccggactcgg gaccgaggaa tgcctggtcc tgcatgccga cctcgaagtc gagcgccacc
     1381 acgatcggcg cggtgggcgc cgggtcgccc cggcggacct gcatcttgcg caccacgttg
     1441 ccgccgtcgt gccgcctgct ctcgtggacg aggggagcat cggcgtccag ggcgtggcgc
     1501 gggtcggtga ccgccggcag caccgcgtac tcgacgatga tcttcttcgc cgcgcggcgg
     1561 gccgtctccg gatggtcggc cgcgaccaga gccaccggct cgccctcgta gcggacgacc
     1621 ccggtcgcca gcgcgggcgt gtcgaggtgc tcgagcccga ccgcgttgct gcccgggatg
     1681 tcggccgcgg tgagcacggc ggacacaccg ggcaccgcga gcgcctcccc gatgtcgatc
     1741 gcgacgatcc gggcatgggg gtgcgggctg cgcagcgtga caccccagac catgtcgtcg
     1801 tgccacagat cgctcgcgta cgcgaactcg ccggtgacct tgagcgcccc gtcagggcgc
     1861 agcggactgt cgccgatgcg gcctgcggtg gtggtcgcgg tggtcgtcgc cgaactcgtc
     1921 tgcgtggacg tcatctcgct cagccggcct tctccaggag tgtgcggtgg acggcggtgc
     1981 actcgcgcgc cagcgcgtcc tcgtcgacgg tgctgacccg gccgccctcg acgaccgcgc
     2041 gcccgttcac gagcagcagc tgaagtggtg gcggcgaccc cagcacgagt gcagccaccg
     2101 ggtcggcgat gtcggcgtgg gcgagaccgt cgatgcgcca gagcgcgatg tcggccagct
     2161 tgccgacctc caccgacccg atctcgtcgg tgcgcccgac cacgcttgcg ccgccgaggg
     2221 tggccagctc cagtgcgtcg cggacggtca gcgcctgcgg cccgcccacc gcacgggcga
     2281 acagcagtgc gtgccgcacc tcctccagca ggctccctgc ctcgttgctc gcggccccgt
     2341 cgacgccgag cgcgacgcgg gcacctgcgg cgcgcaggtc acgggtgcgg gcgatgcccg
     2401 ccccgagccg ggcgttggac gacgggcagt gcgccacgcc ggtggcggtc gccccgatct
     2461 tcttcacggc ctcgtcgtcg aggtgaacgg cgtgggcgaa ccagacatcc tcgccggtcc
     2521 agccgaaccg ctcgacgtac tccagcggcg agcagccgaa ccgctcgcgg cagaaggcct
     2581 cctcgtcgac cgtctcggcg aggtgggtgt gcagcatcac gccacgtcga cgcgcgagct
     2641 ccgccgagtc gcgcatcagc tcccccgtga cgctgaacgg cgagcacggc gcgagcgcga
     2701 cccgcagcat cgcccccggc gacgggtcgt gccagcggtc gatggcggcc tcggaggcgg
     2761 cgagcaccgt gtcgcggtcc tccacgacct cgtcaggcgg gagcccaccg tcgctctggc
     2821 cgagatccat cgagccacgg cacgggtgga accgcagtcc gacgtcctgg gcggcccgga
     2881 tctccgccgc gaacacgtcc ccgccctccc gagggaagac gtagtggtgg tcggtggtgg
     2941 tggtgcagcc cgtgagcgcg agctgggcca gcgctgcgct cgcggcggtg tgcacggcgt
     3001 gctcgtcgat gcccgcccac accgggtaga gcgtggtgag ccattcgaac agcgtcgagt
     3061 cgacggcgag cccacgcgtg acccactggt agaggtggtt gtgggtgttg acgaagccgg
     3121 gcgtgagcag gcagccccgc ccgtcgacga ccaaggcgcc ctcgacgtgg ggtgcgggcc
     3181 cggcggccac cgcggtgatc cggttgtcct ccacgacgac gtggccgccc gggaactcgg
     3241 cgccggagac ggtgacgacg tgggcgccct cgatgaccaa cgcgcgctga gacactgtgg
     3301 tcatgacgca tgctcccccg atgtggtcgc ggcggcgagc cggactgcgt ccatgatctt
     3361 ctcgtagccg gtgcaccggc agaggttccc ggccagcgcc tcgcggatct cgggatccga
     3421 cgggcgcccg ttgcgttcga ggagatcgtg ggtggagacg atcagccccg gcgtgcagaa
     3481 gccgcactgc acggcgccgc actcgaggaa cgcccgctgc acagggtgca gctccccctc
     3541 tgccgagagg ccctcgaccg tggtcacctc gcgcccggcg gcctgcccga ccgcgacgag
     3601 gcaggaacat accggcagtc cgtcgaggta gacggtgcag gatccgcact ccccctgctc
     3661 gcaggcgttc ttcgagccgg gcaaccccat ccgctcgcgg agcgcgtaga gcaggctctc
     3721 gccttcccac acgtcgtcca cgtcgtgctc cgtgccgtta acggtcagcg tgatccgcat
     3781 gtcaggctgc tttccggtag tcgttccatg cccacgtgac ggcgcggcgc gccatcacag
     3841 tgagcgagtg caggcggtag gcggcgctgc cccggacgtc gtcgatcggg gcggccgcag
     3901 cgctcacgcc atggccgaac tcgcgcgcaa ggccgtcggg cagctccccc cgcgactccc
     3961 acagcccggc cgcgtcgagc tcgccggaga gaaactcggc ggcttcgtcg gcccggcgcg
     4021 gggtgggcgc cgccgagccg atcccggtgc ccacagcctt gcggtccggg tgcagcgcga
     4081 gcccgaaggc cgacacggcg atcaccatgg cgttgcgggt accgatcttg cagaactgct
     4141 gcggcccggt cggcgggggg atcagcaccg cggcaatgag ctcgtcgggg gcgagggcgt
     4201 tccgtttcac tcccgtgtag aactgcgcca ccggtacccg ccgcatccca cgcgccgccg
     4261 acgccacctc gacaacggcg tcggccgcaa gcagcggcgg gtggctgtcg cccgccgggg
     4321 acgcggcacc gaggttgccg cccaccgtgc cgcggttgcg gatctgcggc gacccgatcg
     4381 tgcgcgcggc catcgcaagg ccgggcagag tccccccgag ctcgtcgatg acccgggcgt
     4441 aggtgacggc ggccccgagc cggatcatcc cggcgtccgg ggcccactca cgcagctccg
     4501 gcacccgcgt gaggtcgagc agggcgggcg ggcggcgctg gtcgaagttg atctcgacca
     4561 tgacatcggt accgcccgtc acgggcagcg cgccgggatg ctccgccttg gcagcgagtg
     4621 cgtcggccca ggtcgtcggc gcgagaaact ccatgacaga agtagagcgc tcccgaaggc
     4681 cgggtagaag tcaccgccgt gatgaaagtc ggcggcgacc gatcaccccg cgttgtaggt
     4741 tcctccaatg ctgcttcgag acctggtcgg ccatccgggg ctcggtctgc ggctgctgca
     4801 cggcgacgac accgtgctgg accgcccggt ccgctgggtt tacacgaccg acctgatcga
     4861 ccctgctcgc tacctgtccg gcggtgagct ggtgctgagc gggctggtgt ggcgccgcga
     4921 gcccggcgac agtgagcgct tcgtcgcggc gctggcccgg tccggcgccg ccgcgctcgc
     4981 ggcgggggcg gcggtgttcc acggcatccc cgacgacctc gtcgaggcat gccgacacca
     5041 cgacgtccca ctgatcgccg tgccggagga actgtcgttc gccacggtca ccgagctggt
     5101 gatcacctcg ttgactgcgg cgaggggcga ccggctcgcg ctcaccgtgg ggcgccagcg
     5161 tcggctgctc tcggccgtcg cggccgggct cggcctcgac gagctggccg cgcaggtgtc
     5221 ggcgaccacc gggacgacct gccgcgtact cagcaccacc ggccgcgaga tcgccgccgg
     5281 gccgcagccg cttcccgacg acgtgctcga ccggatcgtg gcggcattcc tcacagctga
     5341 ccagctcccc gcgaccctga gcagcgacaa ctacaccgtg ttccccatcg ggccggccgc
     5401 tccgcagcgg ttgacgtcgt ggttcgtcct ggcggacggc gacccggccg gttgggacgc
     5461 cgagaccaac gacaccgtgg gcgagctggc cgccgtcgcg gcgctggacc gggcccggcg
     5521 caatgagggc ctgcggtggg gccgtgacat cgccgacgac gcggtggcgc tcgtcgcggg
     5581 gggcgcgggg aaccggccgg agacgtcggt acggctgcgc caggcggggc tcgatccggc
     5641 ggaaccgctt gtcgtggtga cggcagcgtt cgtcgggcga cccgagctgg tggagacggc
     5701 tcgtgcggtt ctcgtggacg tggcggcgca ccggggcccg gccacagtcg gtgtgcacga
     5761 cggcctggcg gtggcgctgc tgccggggcc cgaccaggac gaaccatccg gcgacctgac
     5821 cggcgccgtg ctgcgccggg cgctccgcag gctcgtcccc gggctggcgg gcctgcgcct
     5881 gacggtcggg gtcagcaggc cgtcgcgacc cgatgcgctg tccggagcgc tgcaggaggc
     5941 ccgccatgcc cgcgacgtcg cggagcagcg gccgggggca ctgcacgtcg tcaccggtgc
     6001 ggagctcacc tcgcacgtgg cgctgctcgc caccgtcccc gatgacgtcc gccgcgcctt
     6061 cgccaggcgt gtgctcggcc ccgtgctcga ccacgacgtc cgcaccggcg caggcctgcg
     6121 ggagacgctc gaggcgttcc tcgactgctc aggctcgtgg acccgtaccg cggagcggct
     6181 gcacctgcac gtcaacaccg tgcgttaccg gatcggccgg gtggagcagc tcaccggccg
     6241 tgacctcggg acacttacgg accgcgtcga cgtcttcctc gcaattcggt cacagatgtc
     6301 atagcattgc acgaacgatc gtgctagaaa ggacgacatg agcagagggg acgcgacccg
     6361 cgagatcatc ctcgacgagg cgggccgtct cgcgcgtcgg gtcggcctcg gcggactgac
     6421 gatcggctcg ctcgccacgc agaccggcat gtccaagagc ggcctgttcg cccacttcgg
     6481 ctccaaggag tcgatgcagc tccaggtgct cgaccacgcc gtcgagcggt tcaccaccga
     6541 ggtgatccgg ccggcgatca aggagccgcg cggggaaccg cgcctgcggg cgctgttcga
     6601 ccactggctc gtgtgggact cgaccgaggg cggctgtccg ctggtcgcgg cgacgttcga
     6661 gctggacgac caacccggcc cagtccgcga gcatctggtg gcgagccagc gcgactggct
     6721 cgacaccatc gcgacggtgg tctccagcgg tatcaccgag ggccacttcc ggtccgacac
     6781 cgaccctcgc cagttcgccc aggacctcga cggagtcatg ttggcctacc acgtcgccag
     6841 ccgcctgctg gccgaccccc aggccgccgc gagggcgcgc cgcgggctgg acgccctgct
     6901 ggccgccgcc gcatgatcgc cgcacgctga ctcccgccgc ctgatccgcc tgttccgagg
     6961 agcgcaccga tggccacgtc gacactcaat agcacgatcg ttcgtgccgc tgacggcgca
     7021 ccgttagtct gggtgcgacc gctgaaccgg caggaggccg ggcacgtcgt cgacgccgtc
     7081 ttcgcccagc tctcgccgca gagccgcttt ctccggttcc actcccccgt cccacggctg
     7141 cccggccccg tccgcgcagg gctcgtcgac ctcgacggcc gctcccgcgc cgcggtcgtg
     7201 gccgagattc ccggcccgga cgggccggaa ccggtcggga tcgcgcgcct ggcctgcacc
     7261 ggagacggga cggccgatgt cgccgtggca gttgtggacg cctggcagcg gcgcggcgtc
     7321 ggcagccggc tcgtcgccgc cctcgccgcg ctcgccgagc agctcggcta cacgaagctc
     7381 tccggcgccg tgctgccgga gaacgtcgcg atgctcggca tggtccgcag cgtgttcccg
     7441 gcagtcctgt cgcactacga cggcgacgtc gtgcaggtcg ctgtccggac cggcacttcc
     7501 gcatgggcgg tcacggagga ggacgtgatg gccgacctgg tgaggcgctg accgatcgag
     7561 agcgacgtgg gagtggtgcg acagcgcctc ccccactctc gtgtcgatct cgacgggttc
     7621 ggtcagctgc gggtgaccgt cgcctcgatc aggccgtagg gacggtccgc cgcgaagaag
     7681 acctcgttcc cgttgtccag gccgaagggg gccaggtcga cggcgaagtg gtgcttgttc
     7741 ggcgcctgca ggttgatctc cgttatcccg gggttggcct cgagcacggc ccggcccatc
     7801 tcccacagcg tctgctggag tgcgcgggag tgcagggtgg cgtactgctc gagaagggtc
     7861 tgccggacct cggaccagct ctttgcccag tccacggacc cgtcccagct gccgtcggcc
     7921 ggtgcagcat ggcgccactg cgccacgagc gcggtggcca tgatccggtc gtcggtctcg
     7981 gcgagcgtcg tgtagcggtc cgtgaggaag cccgcgaact cggagccggt cgatttcagc
     8041 aggacgaggt cgcgcaggcc cgaaaccaca tctgcgcgcc cgccctccac gtcgacgacg
     8101 gtcgtgcgca cctccggacc gcggcgcacc cacgtatgat cgtggccctc cccgccgacc
     8161 tgcacccgct cccaggcgag ctcgtccacc gcgatccggg ctccgcggac cggctccacg
     8221 tcctcgacga aatgacgggc gagggtgagc gcgtactcct cgatctcgcg gagccccttc
     8281 tccttggcga aggcgtaggc cgcgttcttc tgcgagtcgg tcggcaggac ggcggactgg
     8341 tcgccgtgca ggtacgcggc ctcgaagtcg ccgcgcagcg tcgtcgacac cgtgacgtca
     8401 cggatctcgt ggcgggcgct gtcccggtag atccggacga ggcggttctc ggccttcccg
     8461 tactggtggc ctccgagcac gatccccatc tgtctcagct cccgcggtag gtcgagtagg
     8521 cgaacgggga cagcagcaac ggcacgtggt ggtgctcccc cgggtcgatc acactgaacg
     8581 tgacggccac ctcggggtag aacccctgct gcccggtggc cgcgaagtaa ccacccgtgt
     8641 cgaacgtgat ccggtaggcg cccgcggcca ggtcggccgc caggtcgcgg acacggccgt
     8701 cgtcgtcggt gcgggcctcg gccagcggtt cgccgccggg caccgcggtc agccgcacac
     8761 cgacaccggc agcgggtcgc ccgagtgccg cgtcgaggac gtgagtggag agagtcacgg
     8821 cacgtcctgc ccgacgagct ggtcgagccg gatccgattg atggccgcca gttcggcgag
     8881 cgcgaccgcc cgctcggctg ccgggtcgtt agccagccgg gcgtggagga tggccagcag
     8941 gtcctcggcg ctgcgtccgg tggcacacac gaggtagacg cacccgaagc gcccctcgta
     9001 ggcccggttg ccctcctcca acgcggccag cacgtccggt gcggccccac cgacgccgct
     9061 ctgttcgcgc cgggactggc cacccgcctg ccggtcaccg atgcgggggt ggccggccat
     9121 ggcggcgtcg aggtcggcat cgtccagcgc cgctccggca gcggcgtgca gcgcggtgat
     9181 cgaggcgtac gggcggcgcg ccgccaccgt ccgcgcccac cgcggcgcag cgcagcaggt
     9241 gagcagctct cgctcggcct cgtgcggcgg cagcgtgttg aactgctcca gcctcacccg
     9301 tactcccgtc agcgctcgga agatcccgcg ggcagcgggt cgagcacgag accgtccaac
     9361 ttccgccgga ccgtctccag ccgccacttg ttgctgaaca gcgccagcac cacgccatcg
     9421 gcccggcggg tgaggacctc cacctcgctc tggcccttga gcagctcggc tcctaccgca
     9481 tccgtgcggc gcgtaaccga gtagcccagc gtctcgagcg tgaccggggc gcggaactcc
     9541 gactccaggc gagcggtgac cacctcgaac tgcagcgggc ccaccgccgc gagcaccggt
     9601 gcctggtcgc cgcgcaggtc ggagctcagt acctggatga cgccctcgct gtcgagctgc
     9661 tcgatccccc gccggaactg cttgtacgag ccggcgtcgg cgctgcggac cacggcgaag
     9721 tactccggcg cgaagctcgg gatcgcgggg aactccaccg ggtcgctggc gtagagcgtg
     9781 tcaccgggac gcaatgcgcc cgcgttcacc agaccgacga tgtcgccggg gaacgcctcc
     9841 tccacagtgg tgcgctgctg accgaacacg gcctgggcgt acttggtggc gaagggacgc
     9901 ccggtggcgg cgtgggtgag caccatgcca cgttcgaacc gaccggagca gatccggacg
     9961 aacgccatcc ggtcgcggtg ggcgcggtcc atacccgcct gcaccttgaa caccagcccc
    10021 gacgtcggcg cgtccagctc gcgtggcctg ccctcggcat ccggacgcgc ctccggagcc
    10081 ggtgcgagat cgacgagcgc gtcgagcagc cgtgacaccc cgacaccggc cagcgcagcg
    10141 ccgaacagca cgggcgtggc ggaaccgttg aggaagtcct tctcgtcgaa ccccgcgccg
    10201 atctcacgca gcagcgcgag ctcgtcctgc gccgcgggcc agaactcggg ttcctccgcc
    10261 gcgatctgct cggcccgcac cacctccgag gtggcgcggg tggcgccacc cgcggtccgg
    10321 gtgaaccgag tgaactcccc cgtatccgtc tcgacgagcc cgcgcagctc ccccgccacc
    10381 ccgaccggcc aggtgatcgg catggggcgc aaaccgatcg tctgctccac ctcgtcgagc
    10441 agctcgagcg cctcccggcc tggcctgtcc cacttgttga cgaacgtgag caccgggatg
    10501 ccacgggtgc ggcagacgtc gaacagcttg agcgtctgcg gctccaggcc cttcgccgcg
    10561 tcgagcagca tcaccgcggc gtcgacggcg gcgagcacgc ggtaggtgtc ctcggagaag
    10621 tcgccgtggc cgggggtgtc gagcaggttg atcacgcagt cacggtaggc gaactgcagc
    10681 acagccgagg tgatcgagat cccccgcttg cgctccatct ccatccagtc ggaggtgacc
    10741 ccccgcctgc ccgacttgcc gtgcaccgcg ccggcctcgt cgatcacctc ggcatagagc
    10801 gccagtgcct cggtgagcgt cgacttgccc gcgtcgggat ggctgatcac ggcgaacgtg
    10861 cggcgccgct ccgcttcgac cgcaatgcgg gactcccccg atacccgggc cgactccccc
    10921 ggcgacttct ccaccacagc tccagacatc agaccgccga tcctacgtgg cggcgacatc
    10981 tagcttgcga cgagaggctc ccggaccgtg cggtcggcgg cgatgcggtc ggcgacccgg
    11041 caggccagcg cggcgatcgt gagcgtcggc ccgacggcgc ccgcggtcgg gaagacggcg
    11101 ctgcccgcca cgaagaggtt ggcgctcccg tgcacgcggc actcggcgtc gaccacaccg
    11161 tcgcgcggcg agtgcgccat gcgcagcagg cccatctggt gggtgccgtc ggtgaaggcc
    11221 atgtcgtcga tccgcgcggc gtcgtccatc acgaccgtgc cgtacccgag ccggtcgaac
    11281 tcctcgccga tcagcgtcat gctgcggcgg atccggccgc ggtcgtcgcc ggacacgccg
    11341 tagtgcacct gcaggcgccg ctggccgagc tgatcgcgct cgtgcgacag gatcacacgg
    11401 ttcgccgggt ccggggactg ctcagcgtcg aaccggaggc ggcaggtggc gccgtccagc
    11461 ggcatgaacg agggcaggat gcgcctgccg gtgacccgcg gcgcggccca ggacgcggcg
    11521 aagccgccca cccgccgcca gtcgcgtgcg acgttgccga catggcgtcc cagcccggac
    11581 gtgcccgcgt agcgcgcgtg cgtgcccgcc gacttgaagc cgagccgggc ccggttcaag
    11641 gcgtagtagg tgagcgagaa cgtggagagc agcccgtcgc cgtgggacgg gtccgtcggg
    11701 tcggggaacc acagcgcggc cttcaggttg cccacctcgt gccgcgcctg cgccgcgggg
    11761 gtgagggcca gcatccgccg ggcgtacacg ccgtcgcggg tcggcacgta gcccagggcg
    11821 aggttgcggc cgaggtcgct cagcacgacc gtgccgacct cacccaccgg gtgggtcatg
    11881 tagtgccggc cgacctggtc gtgctcgttg ccgacgccgg cgggcgactg ccggttggac
    11941 gcgagcagga tccgcgccga ctcgaggccg cccgcggcga gcacgtaggt gcgcgccccg
    12001 accgtgatct cgacgcccgg gctcggtgcg acgacggcct cgacgatcgc tccgctcacc
    12061 gggtcgcgct ccagccggac gaccgtcgcc tccaccagca gccgcacgtt cgacgccttg
    12121 gccagcaccc gcattcggcc cgcgaacgac gtgggcgggc tccaccgcca ggtcgagtcg
    12181 acatccagcg tctcggacgg cgggacggtg gcggcgggga agcctgcgcg ggctgcggag
    12241 tactcgtact ggcccgcctg caggtgtcgc tgtgcgcgtc ggtagtaggg ccccacctcc
    12301 tcggccgtga tcggccatcc cgcggtgtgc atccactccc gcggcccgag gtcgagctcg
    12361 tcgagcgggg cgcagcgccc gccccacacc gcactggtgc ctccgagccg cttctgccgc
    12421 acggcctcca gcgggtcgtg gagatcaacc gcctccccac ggtacgtgtc cgcctgccgg
    12481 ggcccgaatc gctgaccgcc gccctcgacg accacgacgt cgacgccacg gtgggccagc
    12541 tcggttgcca cgacggcacc tgccggcccc gtgccgatga cgcagacatc ggccgacacg
    12601 cggccccctg gcgcgatccc acgagcatcg atcaacacgg tcatcgagcg tagaggagcc
    12661 tggtttgacc ctttgaatcg gttacgtgaa gactccgccc cggaggtagg agatgcgctt
    12721 tcgcgaaatg gccgcggccg tacgggtgct ggcccgcaac gacctggctc ggtacaacgg
    12781 gtccgggacg tcggagacgg gccggttcga ggacgagctg gggcgaatgc tcggagtcga
    12841 gcacgccctg gcggtgaaca gcgggacgag cgcgctgatc ggggcgctgg tcggggccgg
    12901 gatcggaccg ggcgacgagg tgctcgtgcc cgccttcacc tgggtcgcca cggccgcggc
    12961 gccgctggcg gtgggcgcgg tgccggtgct cgtcgagatc gacgagagcc tgaccatgga
    13021 tccggccgac cttgcgcgca agatcacacc gcacacccga gcggtgatcc cggtgcacat
    13081 gctcaacctc gtgtgcgaca tggactcgat catgaaggtc gccgctgagc acgacctcgt
    13141 ggtgatcgag gacacgtgcc aggcgatcgg cgtccgccac cacggccgca gggtcggctc
    13201 gatcggacac gtcggcgcgt tcagcttcaa ccagcacaag aacatcacct gcggcgaggg
    13261 tggcgcggtc gtgacgaacg acgggcgcat ccacgcgagg gccgcgatgc accacgacgt
    13321 gggcagctac gagcggcccg gatggatcga gaccgaggcg ctattccccg gcgtcaacca
    13381 ccgcatgccc gagctctccg ccgcgatcct gcgcccccag ctggccgcgc tcgacgcgca
    13441 gctggaccgc aggcgccagc gcaggcgaat ggtgctcgac cgcgtcgcct ccggctcgtg
    13501 gacgggcatc acaacgagcc cgcaccacga cgagacggat gctgtcggac tggccctgac
    13561 cttcgaggat ccggcgcagg cccgcgcctt cggcgagctt cgcggcgccc gcaggctgat
    13621 cgacagcggc cgccacgtct acatcaactg gcagtcagtg ctcgcgaagc gctcgttcca
    13681 cccactgttc gatccgcacg cctgggccca ccgcgaggtg gactacggcc cggacagctg
    13741 cccggccacg ctcgagatcc tcgctcgcag ctgcgccgtc gagctggccc cggaggtgcc
    13801 gacacccgcg ttccggttcc tgaccagggg gatgtctcgg tgagctccgc ccccctgccg
    13861 atcggtcgtc agcccttacc ggctgacggg cgccggttct ccccggcgag ccggcgtagc
    13921 tcccgcgaca gcatttccag gccctgcacc tgggcgcgca gcgcctccac ccgactgtcg
    13981 gcggcagccc ggaacacggc gagccgcccc atcagctcag cggtgagctc actgtccgca
    14041 ccggtgtcgt tctcgagccc gtccatggtc gcgagcagct cacgcatctg ctcgaggctg
    14101 aagtcgagcg gtttgagcgt cttgaccagc accaggcggt cgatgtcggc ttcggtgtag
    14161 aggcggaagc cgcccgccga ccgcttcgat ggcacgacga ggccgacctc gtcccagtgc
    14221 cggatcgtcc gcagcgacag gcccacccga gtggcgacct cgccgatctg catcacctgg
    14281 tcgttccttc cccacgaacc acctgcgccc tccgcaagcg gagcacgccg gtagaccggg
    14341 cctcagcgcg tcgaatcgat cgagcttaac cgcccgtcgg gctccgacac ccccaccgag
    14401 gcggagaaga tcggccagcc ggccgttcac acgtccttgg cggcgacgtg cgtgaccggc
    14461 agctggaact tgcgcccgag gcggccgggc aggtcctggt gcaaccaccg agacagccgc
    14521 gccggaagcg tcgacacaat gatctcgtcg aactcccggc tggtcacggc atcctcgacc
    14581 gcacgcatgg gatcggggtc gcacacctcg cccccgatgg tggcgccggc agaggtgaac
    14641 ctcttgatgg ccgcatccag cttggcctgt gccagtcgac gcgcttcggc aggcggcgcc
    14701 gggatcgtcg ggaccccgcc catgaccggc atcgggatcg ccatggcggt caacgcgagg
    14761 tccttcacgg gggttgccgg cacgacgagc cagaactcgg caggaccagc ggccattcgt
    14821 tcattgacga gatccacgag ctcgtcgctg ccgagcgtct ggttcgcgac gaggaggtaa
    14881 cggtgcatga cgcctcccgg gcaagcagtc gggcgtgggc cgtccgcgcc tgcgacacgg
    14941 tggaacggca ccacatcgac gcgggggcac agtaccgctc atcgggcgag gtgagaatgg
    15001 gtacgccccc ggtggaatgg gtgtaggaat gcgcgaatga gcgaaccgga ccgcccgatc
    15061 ttcctcgtgg tcacgatcaa gccgaggctg gaccggatgg cggaggcgga ggcacaactg
    15121 cagtccatgc ggcggaacac gctcaccgag cccggttgcg tcttcatgca cctggtacag
    15181 ccccaggacg atccggaaac gtgggtgatg ctggagatgt ttcgctcccg cgcggcctgg
    15241 gacgagcaca tgcgacagcc ctacaacact gagggcaaca tcatcctgca ggacctgctc
    15301 cgcgaaccct cggacctacg cctgttcgac gagaagcagc cgcgcacgac atgaatgagc
    15361 gtttcacaac tcgtcagtga accggttgcc cacctcaccc gggcgaacac agaacgtagt
    15421 ttctcgctga ttgtctggaa tccggtacag gtgcggacta gcgtccgcac tgctgtggct
    15481 cacgtcacac gctcgcccag tccagcgtct gcgaggggtg atcctatggc tcgcggctcg
    15541 gtcacccggg tgcaaccgcg cgaggagggt gcacggaagc cgggcctggt ctatccgggc
    15601 ctcaccattc cgttcatcct ggtcgtcacc tgcttcgccg cgtggggatc ggcagccaac
    15661 ctcacggatg tcctcgtggg cgtgttccgg cacatcttcg cgatgtcgaa cttccagtcc
    15721 gcgctcgtgc agttcgcgta ctacggcgcg tacttctcac tcgccatccc cgccgcactg
    15781 atcaacagac gctacgggta caaggcgggc gtgctcaccg gtctgggatt ggccaccctc
    15841 ggcggactcc tgttcatccc ggcgagcctg ctgctgacgt acgggttctt cctcatcgct
    15901 ttgttcgtgc tggcgtccgg actgtcgatc ctcgagacct cggccaaccc gttcgtgatc
    15961 gcgatggggc ctgaagcgag cgcgacccag cgactgaacc tggctcagtc gttcaacccg
    16021 gtcggcgcga acatcggtgt cctgctcggc gcggtgttga tcctccctca gatcaccccg
    16081 gaatcggcga aggtatcgat gacggccgag caactgcgac aggtccagga gcaggacctg
    16141 tccctggtgc tcaagcccta caccggaatc gccacggtgc tcctgctgat ctggatcctc
    16201 atcgccttgc gcaagatctc cgtgccggag gagcactccg agttcgggct ggatgcgccg
    16261 ggccgcagtg ggattctcgg tcgcctctgg cacaaccggc actaccgcta cggggtggcc
    16321 gcccagttcc tcaacgtcgg cgcccaggtc tgcacctgga cctttacgat ccagtacgcg
    16381 caggacgtgg tgggcgtgag cacggccaac tccgggtggt acctgcaggc cagcctgatc
    16441 ctgttcctca tctcccggtt cgtgatgacc tatctgctcg gcatcttccg gccgaccaag
    16501 ctgctcttcg ccatgggcgc gctcggtgtg gtgctcgccc ttgtcgccgt gctctcaccg
    16561 aacatggtgg ggttgatcgc cgtcgtcgcg atctcggtct cgctgtccct catgttcccg
    16621 acgatctacg gcgtcgccct acgcggactg ggcaaggaca cgaagttcgg cgctgccggc
    16681 ctcgtcatgg ccatcctcgg cgg
//
LOCUS       DASRSC010000059        10398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3949361,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000059 DASRSC010000000
VERSION     DASRSC010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10398
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3949361"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1547
                     /gene="hpnE"
                     /locus_tag="VFE39_03045"
     CDS             165..1547
                     /gene="hpnE"
                     /locus_tag="VFE39_03045"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HET6257607.1"
                     /translation="MTVRVGVVGGGLAGITAALRCADAGCAVTLLEARPRLGGLAASF
                     HRGELRIDNGQHVFLRCCTSYRQLLDRLGVADLVTLQDRLDIPVHLPGRRPARLRRST
                     LPAPLHLAGALLRYRPLSFPDRVRMVGAALALRQVDPADPATDAISFGDWLAEHGQSR
                     RAVDALWDLVGVATLNATADAASLAPGATVFQLGLLTDATAGDIGWSRVPLGRLHDDA
                     AALGLHDAGVQVHISTKVRALERTAGGWQLTSEREGERVEESVVDQLICAVPPAATER
                     LLPEGAVDLPRGWSAALGSSPIVNVHVVYDRRVLDTPFAAGVDTPVQWVFDRTEQSGL
                     HRVHSAPAQYVAVSLSAADDVIRTPTARLREQMLPALAALLPAARDARVLDFFVTREP
                     HATFRPAPGTGALRPPPHTRTPGLFLAGAWTDTGWPATMEGAVRSGNAAADAALAPAG
                     APSGSVGVAP"
     gene            1544..2572
                     /locus_tag="VFE39_03050"
     CDS             1544..2572
                     /locus_tag="VFE39_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET6257608.1"
                     /translation="MTTSSVSATAVAVLRRCRDTVTPAMQAAVARLDRASYEQASYHL
                     GWTELDGSPGNGGGKAVRPALALLSAEAAGASSATGLPAAVAVELVHNFSLLHDDLMD
                     GDTERRHRRTVWAQWGAPSAILTGDALLALALEVLLEAESRHAAAAAWLVTDTTRELI
                     RGQAEDIEFERRADVGVDECLSMAAGKTGALLAASAEIGAVLAGACPALTTALRSFGA
                     ELGAAFQLVDDLLGIWGDPAVTGKPVLSDLRSRKKSLPVTYAVHHGGAAGRELASWLA
                     GAGGNAEPDLRWAAALVETGGGRDWAAAEARRRMTLAEKALAGVEIPEVTRTELVALG
                     RYLTDREF"
     gene            2572..4470
                     /gene="shc"
                     /locus_tag="VFE39_03055"
     CDS             2572..4470
                     /gene="shc"
                     /locus_tag="VFE39_03055"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HET6257609.1"
                     /translation="MTLTATENTSRSLQGALDAAVGFLRSQQDPQGWWKGELETNVTM
                     DAEDLLLREFLGIREEQETEEAARWIRSQQRADGTWANFRGGPGDLSTTIEAWVALRL
                     AGDPPEAEHMQRAQKFVLAWGGVGSSRVFTRIWLALFGLWSWDELPDMPPELLFLPPW
                     FPLNVYDWACWARQTVVPLTVVATLRPARPLPFGIDELRSPNQGSAPKRASMWSWTGA
                     FQRLDAVLHRYSRHPVRSLRKLAMRRAAEWVLARQEADGSWGGIQPPWVYSLLALHLL
                     DYPLDHPAVKAGLAGLDGFLIREQTPEGWVRRLEACQSPVWDTCLAVTALLDAGVSPD
                     DPGVVRAADWMLGEEIRVPGDWAVRRPNLAPGGWAFEFDNDGYPDIDDTAEVVLALRR
                     VAHPEPVRLRAAVDRAVDWTIGMQSRDGGWAAFDADNTRELITKLPFCDFGAVIDPPS
                     ADVTAHVVEMLVAEGLAGSPSCRRGVAWLLAAQEDDGAWFGRWGANHVYGTGGVVPAL
                     VGAGVPESSPAIRAAVRWLELHQNPDGGWGEDIRSYTDPAWIGRGTSTASQTAWALLA
                     LLAAGERTVAVERGVRWLVDSQLDDGSWDEPEFTGTGFPGAFYINYHLYRMVFPLSAL
                     GRYLAGVR"
     gene            4467..5963
                     /gene="ispH"
                     /locus_tag="VFE39_03060"
     CDS             4467..5963
                     /gene="ispH"
                     /locus_tag="VFE39_03060"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET6257610.1"
                     /translation="MTAPVVCTPLRVERAALGGRLGAVEVLRTGPGPRRSTAAAARIG
                     ASAPRPVLVAGVAGALDPRLRPGDLVVADEVWCEAGEPVPVATAGPLAAALRRLGLRV
                     HVGPVVSSPVVVDGPRRAGLAASGALAVDMESAWLAAAAAGGPFAVVRAVVDTAEAPL
                     YRLGTAGRGLAALRSLRCAGPALQEWLAATGPRTVELAQPRSFCAGVERAIDVVDRAL
                     HRYGAPVYVRRQIVHNAHVVRGLESRGAVFVQEVEEIPRGAVAVLAAHGVAPAVRAEA
                     EVRDLSVIDATCPLVAKVHTEVRRYSERGDTVFLIGHADHEEVEGTLGEAPDHVVVIE
                     DVAGAAAVEPPDAGRVAYAMQTTLAVDEAEEIAAVLRGRFPGLAGPRRDDICYATTNR
                     QRAVRVVAQRVDLLVVVGSPNSSNSLRLVEVARREGVPAHLIDDVGELDLRWLAGVRR
                     VGVSAGASAPEHLVDDVVSCLSGLGPVTVTTATTATEDVSFTLPKEVS"
     gene            5966..6970
                     /gene="hpnH"
                     /locus_tag="VFE39_03065"
     CDS             5966..6970
                     /gene="hpnH"
                     /locus_tag="VFE39_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HET6257611.1"
                     /translation="MAMPMRQSIRLAAYLMKQKIKRVEKFPLLVELEPLFACNLKCAG
                     CGKIEQPAALLKQRMPVEQAVGAIEESGAPMVSIAGGEPLMHKEIDEIVRQLLDRGKI
                     VFLCTNAVLLPKHLHRFTPHKNFAWMVHIDGLRERHDASVCKDGVFDQAVAAIKMAQE
                     KGFRVNTNTTFFTTDTPHDVIDVLDYLNDELGVDNMQISPAYAYEKAPDQEHWLGVEQ
                     TRELFRKAFDGGRRKRWRLNHSPLFLDFLEGKIDLACTAWAIPSYSLLGWQRPCYLLD
                     DGYVRTYKELVEDTDWSAFGRGNDPRCENCMAHCGYEPSAVFATLGSLRESLRAAAGT
                     "
     gene            7070..7729
                     /locus_tag="VFE39_03070"
     CDS             7070..7729
                     /locus_tag="VFE39_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HET6257612.1"
                     /translation="MLQPRITIHLPSFGSLLRHAGVPLLESTLIPLALFWVLFTQAGF
                     DAGLFGALGWSALAIGRRLVLRRRLPAVLLISTVLLVARTVVGLWTGSAFFYFLQPTV
                     QNFVFALALLVTLGFERPLLAKLADDFCPFPEALTGHPRIKRFFKRVSLLWAFVFLVN
                     GATTLAVLTTQTVGNYLMVSTAGSYSMVALGIGLSLWWFRRSLAGEGIRLRIGAPSPA
                     V"
     gene            complement(7756..8895)
                     /locus_tag="VFE39_03075"
     CDS             complement(7756..8895)
                     /locus_tag="VFE39_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6257613.1"
                     /translation="MGDAGGANRAAGPTRVLLLSAPVGEGHVAAARALAARMRVLWPE
                     AEVREVEGTGRGGWWRDALLRVSYGATLRFAPRLYGLGYDLLVRYPKVAEVFKNLSSG
                     SIGRALEPLLAADPPDLVVSTYPMISGGLAWLRRRGRLPGRAVAVVTDVAVHPFWVWP
                     DLDETWTLLPASQAQARAVAPRADVRVVPAAVDRRFRPGDPVAARIATGMRPDAFVVL
                     VTGGSLGFGGLETVVDAVLAGGQDVQAVVLCGRNGRLRARLLARGLPVDRLVALGWTD
                     RVAEAITAADVVVTTAGGMIATEALAVGRPVLFAAPIPGHGRAGAAMTAAAGLAVVCP
                     TPAEVTAVVRRLEGDPGERAALAGRAAEFGRADLDAELTALALRV"
     gene            8937..>10398
                     /locus_tag="VFE39_03080"
     CDS             8937..>10398
                     /locus_tag="VFE39_03080"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257614.1"
                     /translation="MSAGTDTGTESRTTPAPATGAARPPRLLLVTGSMGEGHHAAARA
                     VEERARRAWPGVEVVWSDTLDGMGRGTGPVFRAVYAGCVRRLPWLYELYFWLLWHVRP
                     FRAGTRAVIGTWSARGLATQLARHSPDLVVATFPEGITGLARLRRRGGLPMPTVALVT
                     DPAPHPLWADAALDLHLVSTSAGAALLRRAAAGARVRVAALPVSSRFTPPPAGAARER
                     PLVYVSCGSMAFGDVAAACTAALGSGADVLVSCGRDAAVRRRLARVARGHPRGAAMRV
                     VDWVDDPAAATRDCDVVVTNAGGATALEALACARPLLMFAPIPGHGRANAEVLAESGL
                     ARICPEPAGLAVALGELADPGRRNALVRFLLARLAGTDLDADVAALADLRTRGPHSVR
                     VRAQDAMFLHVATAQVPQQIGARILVEDPTGRDDWPEHLAGLVRARVPHIELLRRRLQ
                     RPILGQPLRWITDDVPDPARHVRPGIVEIGAGGGPPT"
BASE COUNT         1315 a         3826 c         3795 g         1462 t
ORIGIN      
        1 ggccgatccg ccgtccgtgt accaccagcg gcggtcgctc tcggggtggc agaaggccgc
       61 ggtggcagcg cgcgcgctga gcgggcgggc gcccgcggga gccgcgacat gagccccgtc
      121 cccgcgagct gcccggtggg ccctgaccct gaggacgctg cgtcatgacc gtcagggtcg
      181 gggtggtcgg cggcggcctc gccgggatca cggccgccct ccggtgcgcc gacgccggct
      241 gtgcggtgac gctgctggag gcgcgtccgc ggctcggcgg cctcgctgcc tcgttccacc
      301 gaggagagct tcgcatcgac aacgggcagc atgtgttcct gcgctgctgc acctcctacc
      361 gccagctgct ggaccggctc ggcgtggcgg acctggtgac gctgcaggac cggctggaca
      421 tccccgtgca cctgcccgga cggcggccgg cccgactccg gcgttccacc ctgcctgcgc
      481 ccctgcatct cgcgggggcg ctcctgcggt accggccgtt gtcctttccg gaccgagtgc
      541 ggatggtcgg ggcggccctc gcactgcggc aggtcgaccc ggccgatccc gcgaccgacg
      601 cgatcagctt cggcgactgg ctcgccgagc acggccagag ccgccgtgcg gtcgacgcgc
      661 tgtgggacct ggtgggtgtg gccacgctca acgccacggc ggacgccgcc tcgctcgcgc
      721 ctggcgccac cgtgttccag ctcgggctgc tcaccgacgc gacggcgggg gacatcggct
      781 ggtcccgggt gccgttggga cggctgcacg acgacgcggc cgcactcggc ctgcacgacg
      841 cgggcgtgca ggtgcacatc tcgacgaagg tccgcgcgct ggaacggacg gcgggcggct
      901 ggcagctcac ctcggaacgc gagggcgaga gggtggagga gtcagtggtc gatcaactca
      961 tctgcgccgt gccgcccgcg gcgacggaac ggctgctgcc cgaaggggcg gtggacctgc
     1021 cacgtgggtg gtccgcggcg ctgggcagct caccgatcgt caacgtgcac gtcgtctacg
     1081 accggcgggt tctcgacacc cccttcgccg cgggggtcga caccccggtg cagtgggtct
     1141 tcgaccgcac cgagcagtcg gggctgcacc gggtgcattc cgcacccgcg cagtacgtcg
     1201 cggtctccct gtctgccgcc gacgacgtga tccgcactcc cacggcccgg ttgcgcgagc
     1261 agatgctgcc ggcgctggcc gcgttgctgc ccgcggcccg ggatgcccgg gtgctcgact
     1321 tcttcgtcac gcgggaaccg cacgcaacgt tccggccggc cccgggtacg ggcgcgctcc
     1381 ggccaccgcc gcacaccagg accccgggtc tgttcctcgc cggcgcgtgg accgacaccg
     1441 ggtggcccgc caccatggag ggcgctgttc gcagcgggaa cgccgcagcc gacgccgcac
     1501 tcgcccccgc gggcgccccg tcaggctcgg tgggtgttgc gccatgacca cgtcctccgt
     1561 ctccgccacc gcggtcgccg tgctccgacg ctgccgcgac accgtcaccc cggccatgca
     1621 ggcagccgtc gcgcggctcg accgggcgag ctacgagcag gcgtcctacc acctggggtg
     1681 gaccgagctc gacggctcac ccggaaacgg cggcgggaag gcggtgcggc ccgcgctcgc
     1741 gctgctgtcc gccgaggccg ccggggcgtc cagcgcgacc ggcctgcccg cggcggtcgc
     1801 cgtcgagctg gtacacaact tctcgctgct gcacgacgac ctcatggacg gcgacaccga
     1861 gcgcaggcac cggcgcacgg tgtgggcgca gtggggggct ccgtcagcga tcctgaccgg
     1921 ggacgcactg ctcgcgctgg ccctcgaggt gctgctggaa gccgagtcgc ggcacgcggc
     1981 ggctgcggcc tggttggtca ccgacaccac tcgagagctg atccgcggcc aggcggagga
     2041 catcgagttc gagcggcgcg ccgacgtcgg tgtggacgaa tgcctgtcga tggccgccgg
     2101 caagaccggt gcactgctgg ccgccagcgc ggagatcggg gcggtgctgg ccggtgcctg
     2161 ccccgcgttg accaccgcac tgcgtagctt cggcgccgag ctgggagcag cgttccagct
     2221 ggtcgacgat ctgctcggca tctggggtga ccccgctgtc accggcaagc cggtgctgtc
     2281 ggacctgcgc tcgcgcaaga agtcgctgcc ggtgacctac gcggtgcacc acggcggggc
     2341 ggccgggcgg gaactggcga gctggctggc cggagccggc gggaacgcgg agccggacct
     2401 ccggtgggcg gccgcgctcg tcgagaccgg tggtgggcgg gactgggccg ccgccgaggc
     2461 acgcaggcgg atgacgctgg ccgagaaggc gctggccggc gtcgagatcc ccgaggtcac
     2521 ccggactgag ctcgtcgcac tcggccgcta cctcaccgac cgggagttct gatgaccctc
     2581 accgcaactg agaacacctc acgcagcctg cagggtgccc tcgacgccgc cgtcggcttc
     2641 ctgcgctcac agcaggaccc gcagggctgg tggaagggcg agctcgagac gaacgtgacg
     2701 atggacgccg aggacctgct gctgcgcgag ttcctcggca tccgcgagga gcaggagacc
     2761 gaggaggcgg cgcgctggat ccgctcacag cagcgcgccg acggcacctg ggcgaacttc
     2821 cgcggcggtc ccggcgacct gtcgaccacg atcgaggcct gggtcgcgct ccggttggcc
     2881 ggtgacccgc cggaagcgga gcacatgcag cgcgcgcaga agttcgtgct ggcctggggt
     2941 ggtgtgggga gcagccgcgt gttcacgcgg atctggctcg cgctgttcgg gctgtggtca
     3001 tgggacgagc tcccggacat gccgcccgag ctgctgttcc tgcctccctg gttcccgctc
     3061 aacgtctacg actgggcgtg ctgggcccgg cagaccgtgg tgccgctgac cgtcgtggcc
     3121 accctgcggc cggcccgccc gctgcccttc ggcatcgacg agctgcgcag cccgaaccag
     3181 ggcagtgcgc cgaagcgggc ctcgatgtgg tcctggaccg gcgccttcca gcgtctcgac
     3241 gcagtgctgc accgctactc ccggcacccc gtgcggtcgc tgcgcaagct ggcgatgcgc
     3301 cgggccgccg agtgggtgct ggcccgccag gaggccgacg gatcctgggg cggcatccag
     3361 ccgccgtggg tgtactcgct actggccctg cacctgctcg actacccgct cgaccacccg
     3421 gctgtgaagg caggcctcgc cgggctcgac ggcttcctga tccgcgagca gacccccgag
     3481 gggtgggtcc gcaggttgga ggcgtgccag tcgccggtgt gggacacctg cctggcggtc
     3541 acggcgctgc tcgacgccgg ggtctccccc gacgacccgg gggtggtgcg cgccgcggac
     3601 tggatgctcg gcgaggagat ccgcgtgccg ggcgactggg ccgtacgcag gccgaacttg
     3661 gctcccgggg gatgggcgtt cgagttcgac aacgacggct acccggacat cgacgacacg
     3721 gccgaggtcg tgcttgcact gcgtcgcgtc gcccatcccg agcccgttcg gctccgcgct
     3781 gcggtcgacc gcgcggtgga ctggactatc ggtatgcagt cgcgcgatgg cggctgggcc
     3841 gcgttcgacg ccgacaacac gcgcgagctg atcacgaagc tgcccttctg cgacttcggt
     3901 gccgtgatcg acccgccgtc ggccgacgtc accgcccatg tggtggagat gctcgtggcg
     3961 gaggggctcg cggggtcacc gtcctgccgc cggggcgtcg catggctgct cgccgcgcag
     4021 gaggacgacg gagcgtggtt cggccgctgg ggggcgaacc acgtctacgg cacgggtggg
     4081 gtcgtccctg ccctcgtcgg cgccggggtg cccgagtcgt cgccggcgat ccgcgctgcg
     4141 gtgcgctggc tggagctcca ccagaacccc gacgggggct ggggcgagga catccggtcc
     4201 tacaccgacc cggcgtggat cggtcgcggc acctccacgg cgtcccagac ggcgtgggcg
     4261 ctgctcgcgc tgctggcggc aggcgagcgg accgtggccg tcgaacgcgg tgtccggtgg
     4321 ctggtcgaca gccagctcga cgacggctcc tgggacgagc cggagttcac cggcaccggc
     4381 ttccccggcg cgttctatat caactaccac ctctatcgca tggtcttccc gctctccgcg
     4441 ctcggccggt acctggccgg ggtccgatga cggcgcccgt cgtctgcacg ccgctccgcg
     4501 tggagcgggc cgccctcggc ggcaggctcg gcgcggtcga ggtcctccgc acggggccgg
     4561 gcccccgccg ctccacggcc gccgcggctc gcatcggggc gagcgccccc cgccccgtcc
     4621 tcgtcgccgg cgtcgcgggc gcgctcgacc cccggctccg gccaggtgac ctcgtggtcg
     4681 ccgacgaggt gtggtgcgag gcgggcgaac cggtcccggt ggcgaccgcg ggcccgctgg
     4741 cggcggccct gcgccggctc gggttgcgcg tgcacgtcgg cccggtggtc tccagccctg
     4801 tcgtggtcga cgggccccgg cgggccggcc tcgccgcgag cggggcgctg gccgtcgaca
     4861 tggagtcggc ctggcttgct gccgcggcgg ccggtggccc gttcgccgtg gtccgtgccg
     4921 tggtcgacac cgcggaggcg ccgctctacc ggctcgggac ggccgggcgc gggctggccg
     4981 cgctccggtc cctgcgttgc gcagggcctg cactccagga atggctggcc gccaccggac
     5041 cgcgcacggt cgagctggcg cagccgcggt ccttctgcgc gggtgtggag cgcgccatcg
     5101 acgtcgtcga ccgggcgctt caccgctacg gcgcgccggt ctacgtgcgc cgccagatcg
     5161 tgcacaacgc ccacgtggtg cgcgggctcg agagccgcgg cgcggtcttc gtccaggagg
     5221 tcgaggagat cccccgcggg gcggtcgccg tgctcgccgc gcacggggtc gcgcctgcgg
     5281 tccgcgccga ggccgaggtg cgcgacctgt ccgtgatcga tgcgacgtgt ccgctcgtgg
     5341 ccaaggtgca caccgaggtg cgccgatact ccgagcgcgg cgacacggtg ttcctcatcg
     5401 gccacgccga ccacgaggag gtcgaaggca ccctcggtga ggctcccgac cacgtcgtcg
     5461 tcatcgagga cgtcgccggg gcggcagccg tggagccgcc cgacgcgggc cgggtggcct
     5521 acgcgatgca gaccacgctg gccgtcgacg aggccgagga gatcgcggcg gtgctgcgcg
     5581 gccgcttccc ggggctggcc gggccccgcc gcgacgacat ctgctacgcc actacgaacc
     5641 ggcagcgagc ggtgcgcgtg gtcgcgcaga gggtcgacct gctggtcgtc gtcggctcgc
     5701 cgaactcctc gaactcactc cggctcgtcg aggtcgcgcg ccgcgagggc gtgccggcgc
     5761 acctcatcga cgacgtcggt gagctcgacc tgcgctggct cgcgggggtg cgccgggtcg
     5821 gcgtcagcgc aggcgcgtcc gcgcccgagc acctcgtcga cgacgtggtg tcctgcctgt
     5881 ccggtctcgg accggtgacc gtcacgacgg ccaccaccgc aaccgaggac gtctcattca
     5941 ccctgcccaa ggaggtgagc tgaccgtggc aatgcccatg cgccagtcca ttcgactcgc
     6001 cgcgtacctg atgaagcaga agatcaagcg ggtggaaaag ttcccgctgc tcgtcgagtt
     6061 ggagccactg ttcgcctgca acctcaaatg cgcgggctgc ggcaagatcg aacagcccgc
     6121 agcgctgctc aagcagcgga tgccggtgga gcaggccgtc ggtgcgatcg aggagagcgg
     6181 cgcgccgatg gtgtcgatcg ccggcggcga gccgctgatg cacaaggaaa tcgacgagat
     6241 cgtccggcag ctgctcgacc gcgggaaaat tgtcttcttg tgtacgaacg cggtgctcct
     6301 gcccaagcat ctgcaccggt tcaccccgca caagaacttc gcgtggatgg tgcacatcga
     6361 cggcctgcgc gagcgccacg acgcctcggt gtgcaaggac ggggtcttcg accaggccgt
     6421 cgccgccatc aagatggctc aggagaaggg cttccgggtc aacacgaaca ccaccttctt
     6481 caccaccgac acaccccacg acgtgatcga cgtcctcgac tacctcaacg acgagctcgg
     6541 cgtcgacaac atgcagatct cgccggccta cgcctatgag aaggcgcctg accaggaaca
     6601 ctggctcggc gtcgaacaga ctcgcgagct gttccgcaag gccttcgacg gcggcaggcg
     6661 caagcggtgg cggctcaacc actctccgct cttcctcgac ttcctcgagg gcaagatcga
     6721 tctcgcctgc acggcgtggg cgatcccgtc ctactcgctg ctgggctggc agcggccctg
     6781 ctacctgctc gacgacggct acgtgcggac gtacaaggag ctggtcgagg acaccgactg
     6841 gtcggcgttc ggccggggca atgatccgcg ctgcgagaac tgcatggccc actgcgggta
     6901 cgagccgtcg gccgtcttcg ccacactcgg ctcgttgcgg gagtcgttgc gcgccgctgc
     6961 gggtacctga gagagggtga ccggcagatc cggacgatga gcccgttatc gccccgttac
     7021 cggcctcagt acactgcttc caccccgagg tcggccggag aggcagagca tgctgcaacc
     7081 ccgcattacg atccacctcc cttcattcgg atcgttgcta cggcatgcgg gcgtcccgct
     7141 gctggagtcc acgcttattc cgctcgccct gttctgggtg ctcttcaccc aggccgggtt
     7201 cgacgccggc ctgttcggcg ccctgggctg gtccgcgctc gcgatcggcc gcaggctggt
     7261 cctgcgtcgg cggctgccgg cggtcctgct catcagcacg gtgctgctgg ttgcgcgcac
     7321 cgtggtgggg ttgtggacgg ggagtgcgtt cttctacttc ctgcagccca cggtgcagaa
     7381 cttcgtcttc gcgctggcgt tgctggtcac cctgggtttc gagcgcccgc tcctggccaa
     7441 gctggccgac gacttctgcc cgttcccgga ggccttgacc gggcaccccc gcatcaagcg
     7501 gttcttcaaa cgggtgtcgt tgctgtgggc cttcgtgttc ctggtcaacg gcgccacgac
     7561 gctcgccgtc ctgaccaccc agacggtcgg caactacctg atggtctcga cggcggggtc
     7621 ctactccatg gtcgcactgg ggatcgggct gtcgctgtgg tggttccgcc gctccctggc
     7681 gggcgagggc atccggctgc ggatcggcgc acccagcccg gcggtctgac gccgggcggg
     7741 ccgagccggc gcggctcaga cccgcagggc cagcgcggtc agctccgcgt cgaggtcggc
     7801 ccggccgaac tcggccgcgc ggcctgccag ggcggcccgt tcgccgggat ccccctcgag
     7861 cctgcggacc accgccgtca cctcggcggg cgtagggcag accaccgcga gcccggccgc
     7921 ggcggtcatc gcggcaccgg cccggccgtg tccggggatg ggtgccgcaa acagcacagg
     7981 gcgcccgacg gccaacgcct ccgtcgcgat catgccgcct gctgtcgtca cgaccacgtc
     8041 cgcagcggtg atcgcctccg cgacccggtc ggtccagccg agcgcaacca ggcgatcgac
     8101 cggcagacca cgggcgagca gccgggcccg cagccgcccg ttgcggccgc acaggaccac
     8161 cgcctgcacg tcctgaccac cggcgagcac cgcgtcgacc accgtctcca ggccgccgaa
     8221 gcccagcgaa ccgccggtga ccagcacaac gaacgcgtcc ggccgcattc cggtggcgat
     8281 ccgggcggcg accgggtcgc ccggccggaa acgccggtcg accgccgccg gcaccacccg
     8341 gacgtctgca cgcggcgcca cggccctggc ctgggcctgg gacgccggca gcagcgtcca
     8401 cgtctcgtcg aggtcgggcc acacccagaa cgggtgcacg gcgacgtccg tgaccaccgc
     8461 caccgcgcgg cccggcaacc gcccgcgcct gcgcagccag gccaggccgc cggagatcat
     8521 cgggtaggtc gagacgacga gatcgggggg atcggccgcg aggagcggtt cgagcgcccg
     8581 gccgatgctg cccgaggaca ggttcttgaa cacctcggcg accttcgggt accggacgag
     8641 caggtcgtag ccgagcccgt agagccgggg ggcgaaccgc agcgtcgcgc cgtaggagac
     8701 ccgcagcagc gcgtcacgcc accagccgcc gcgtcctgtg ccctcgacct cccgcacctc
     8761 ggcctccggc cagagcacgc gcattcgcgc cgcgagcgcc cgggcggcag cgacgtggcc
     8821 ctcaccgacc ggcgcggaga gcaacagcac ccgcgtcggc ccggccgcgc ggttcgctcc
     8881 tccggcatcg cccacatcgg cacgctagcg cggcgacgat ctcccggtgg ggtggaatga
     8941 gcgccggtac cgacacgggc accgagagcc ggaccacgcc ggctccggcg accggagccg
     9001 ctcgtccgcc gcgcctcctg ctggtcacgg gcagcatggg cgagggccat cacgcagccg
     9061 cccgcgccgt cgaggaacgg gcgcgccggg cctggccggg ggtggaggtg gtgtggtccg
     9121 acacccttga cgggatgggc cgcggcaccg gacctgtctt ccgagcggtt tacgccgggt
     9181 gcgtgcgcag gctgccgtgg ctgtacgagc tgtacttctg gttgctctgg cacgtgcgcc
     9241 cgtttcgcgc cgggacccgt gccgtgatcg gcacgtggag cgcccgcggc ctcgccaccc
     9301 agttggcccg gcactccccc gacctcgtgg tcgctacgtt cccggaaggg atcaccgggc
     9361 tcgccaggct ccgtcgccgg ggcgggctgc cgatgcccac cgtcgccctc gtgaccgacc
     9421 ccgcgccgca cccgctgtgg gcggacgctg cgctggacct gcacctcgtg tcaacgagcg
     9481 ccggggcggc gctgctgcgc cgcgccgccg cgggcgcgcg ggtacgggtg gcggccctgc
     9541 cggtctcgag ccggttcacc ccgccgccgg caggcgccgc ccgggaacgc ccgctggtct
     9601 acgtgtcctg cggctcgatg gccttcggtg acgtcgccgc ggcatgcacc gccgccctcg
     9661 gcagcggggc cgacgtgctc gtgagctgcg ggcgcgacgc ggccgtgcga cggaggctgg
     9721 cgcgggtcgc acgcggccat ccgcgcggcg cggcgatgcg ggtggtcgac tgggtggacg
     9781 acccggccgc cgccacccgc gactgcgacg tcgtggtgac gaacgccggg ggggccactg
     9841 cgctggaggc gctggcctgc gcccgcccgc tgctgatgtt cgccccgatt ccgggccacg
     9901 gcagggcgaa cgccgaggta ctcgccgaat cgggcctggc caggatctgc ccggagcccg
     9961 ccggcctggc cgtcgcgctc ggcgagctcg ccgatcccgg cagacgcaac gccctggtcc
    10021 ggttcctgct cgcgcgcctc gccggcaccg acctcgacgc cgacgtcgcc gcactcgccg
    10081 acctgcgcac gcgcggcccg cacagcgtgc gggtgcgcgc acaggacgcg atgttcctgc
    10141 acgtcgcgac cgcgcaggtg ccgcaacaga tcggggcgcg catcctcgtc gaggatccga
    10201 cgggccgcga cgactggccc gagcacctcg ccgggctggt ccgggcccgg gtgccgcaca
    10261 tcgagctgct ccgtcgccgg ctgcagcgtc ccatactcgg acagccgttg cgctggatca
    10321 ctgatgacgt gccggaccct gcccggcacg tacggcccgg catcgtcgag atcggggccg
    10381 gcggtggacc acccacct
//
LOCUS       DASRSC010000060         2796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1329818,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000060 DASRSC010000000
VERSION     DASRSC010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329818"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /locus_tag="VFE39_03085"
     CDS             <1..805
                     /locus_tag="VFE39_03085"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6257615.1"
                     /translation="ALDDRLESFVPDIPNGPDITVRQLLNMTAGVYDFTTNPEFTTSF
                     DADPPAPFPVDRVLAILRRSPPTFPPGQPGSWQYSDSNYILLGQIIEEAAGEPIGTFI
                     EREIVGKLRLPGTSYPRSPAIPPPASRGYLVVPPGAPERDVTAVNPDVPGPAGAMIST
                     LAGLRAWAGALAKGRLLSAASHAEQTTFVNANLSPALRTGYGAGLFKINDFVGHNGAI
                     YGFNTAMFQLPETGATVVVVANRSSNGEGVGLETFLLIAKLLYPDRFSS"
     gene            802..1947
                     /locus_tag="VFE39_03090"
     CDS             802..1947
                     /locus_tag="VFE39_03090"
                     /inference="COORDINATES: protein motif:HMM:NF021599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HET6257616.1"
                     /translation="MTDAPVTARSVVAGQARVIAAAWSPDDAPAGWWLTAETFTAIAE
                     EDDLLDLAAAIPPDRLPPLLLGAAVAFLVRERRAAVGRYYPEPGGGQPRRDGGFRAEL
                     VDFVRAERGALGELCATHRYQMNEVARCADVLPVLATIGTEDMRPLALVDLGTGAGLG
                     LHLDRYHYTYRSAGTPDRTVGDRTSPVLLTCEVRPGGRPPVPAELPVVTDRVGVDVEP
                     LDVADSHTLAWLAACVPPESGAVTRFAAAAAVARADPARSVRGNLLEVLGDVVAATPR
                     DALVCLVDTYVHVFLPPADLQRFDDLLTELGRERDLEWISVDPLVPLGPDARASVQGL
                     DVPAAWVRDTRDGGVFGVIGRVRVRAGRRTGTLLGRAHPGAAWLEWR"
     gene            complement(2002..2778)
                     /locus_tag="VFE39_03095"
     CDS             complement(2002..2778)
                     /locus_tag="VFE39_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6257617.1"
                     /translation="MNRFAPGTFTPQPGAGAPLRMLAAQSGTELRLALRNGEQVLITL
                     LIPIVLLVGLSTLDVVPLPEPRIAAVVPGVLALAVMSTAFTGQAIALGFDRRYGVVRR
                     LAATALPRWLLVAGRLVAVLGIVALQVVVLCALAMALGWRPEPSGLGWALLLVLLGCA
                     AFGSLGILLGGSLRAEITLAVANLVWFVLLLAGGIVVPLGMLPGPLAVVGAVLPSGAL
                     AEGLRTALSAGQAPSAAQVLVLVGWTLVASALALRTVKLR"
BASE COUNT          396 a         1062 c          973 g          365 t
ORIGIN      
        1 ggccctcgac gaccggctgg agtccttcgt gccggacatc cccaacgggc ccgacatcac
       61 cgtccggcag ctgctgaaca tgaccgcagg cgtctacgac ttcaccacga atccggagtt
      121 caccacgtcc ttcgacgccg acccgccggc tcccttcccg gtcgaccgag tgctcgcgat
      181 cctgcggcgt agcccaccga cgttcccgcc gggacagccc gggagctggc agtacagcga
      241 ctccaactac atcctcctcg gacagatcat cgaggaggcg gccggtgagc ccataggcac
      301 gttcatcgag cgcgagatcg tcggcaagct gcgtctgccg ggcacgagct accccagatc
      361 acccgcgatt ccgccgcccg cctcccgcgg ttacctggtc gtaccgccgg gtgcgccgga
      421 gcgcgacgtc accgcggtca acccggacgt gccgggcccg gccggggcga tgatctccac
      481 ccttgccggg ctgcgggcct gggccggagc gctcgcgaag ggcaggctgc tcagcgccgc
      541 gtcccacgcc gagcagacga ccttcgtgaa cgcgaacctc tcgcccgcgc tacggaccgg
      601 ctacggtgcg ggcctgttca agatcaatga cttcgtcggg cacaacggcg ccatctacgg
      661 cttcaacacc gcgatgttcc agctgcccga gaccggtgcc acggtcgtcg tggtggcgaa
      721 caggtcgtcg aacggcgagg gtgtggggct ggagacgttc ctgctgatcg cgaagctgct
      781 ctacccggac aggttctcct cgtgacggac gctccggtga ccgcccgctc cgtcgtggcc
      841 gggcaggcgc gcgtcatcgc tgctgcctgg tcgcccgacg acgccccggc cggctggtgg
      901 ctcacggccg agaccttcac ggcgatcgcc gaggaggacg accttctcga cctggccgca
      961 gcgatcccgc ccgaccgcct cccgccgctg ctgctcggcg cggcggtcgc cttcctggtg
     1021 cgcgagcgac gggcggcggt gggccgctac tacccggaac caggtggcgg acagccccgg
     1081 cgcgacggtg gcttccgtgc cgagctcgtc gacttcgtgc gggccgagcg gggtgcgctc
     1141 ggggagttgt gcgccaccca ccgctaccag atgaacgagg tcgcccgctg cgccgacgtg
     1201 cttcccgtgc tcgcgacgat cggtacggag gacatgcgcc cgctggcgct ggtggacctg
     1261 gggaccggcg ccggcctcgg gctgcatctg gaccgctatc actacaccta tcggagcgcg
     1321 ggcacgcccg accggaccgt gggggatcgc acgtccccgg tgctgctgac ctgcgaggtc
     1381 cgacccggcg ggcggccgcc cgtgcccgcc gagcttcccg tggtgaccga tcgcgtcggg
     1441 gtggacgtcg agccgctcga cgtcgccgat tcgcacaccc tcgcctggct ggcggcatgc
     1501 gtcccgcccg agtccggcgc cgtcacgcgg ttcgcggccg ccgccgcggt ggcacgcgcc
     1561 gacccggccc gctccgtacg cgggaacctg ctcgaggtgc tcggcgacgt cgtcgcggcg
     1621 accccgcgcg acgcgctggt ctgcctcgtc gacacctatg tgcacgtctt cctgccgccg
     1681 gccgacctgc agcggttcga cgacctcctg accgagctcg ggcgggagcg tgacctggaa
     1741 tggatctcgg tcgacccgct cgtgccgctc gggcctgatg cccgggccag cgtgcagggc
     1801 ctggacgtgc ctgcggcatg ggtgcgcgac acccgcgacg gtggtgtctt cggcgtgatc
     1861 ggtcgggtcc gcgtgcgggc gggtcggcgt accggcacgt tgctcggccg ggcgcacccc
     1921 ggcgccgcct ggctggagtg gcgctaggag cgcgctgagc cactccgccc gtagtggttc
     1981 agcaccctcc tgccgcccgc gtcaccgcag cttgacggta cggagcgcca gcgcagacgc
     2041 aacgagtgtc cagcccacga gtacgagcac ctgcgcggcg ctcggtgcct gcccggccga
     2101 cagggccgtc cgcaggccct ccgccagcgc gccggacggc agaacggcgc ccaccacggc
     2161 cagcggcccg gggagcatcc cgagcgggac gacgatcccg ccggccagca gcagcacgaa
     2221 ccacaccagg ttggccacgg cgagcgtgat ctcggcccgc aacgaaccgc cgagcaggat
     2281 ccccagcgag ccgaacgccg cgcagccgag caacaccagc agcagcgccc agcccagccc
     2341 ggacggctcc ggccgccagc ccagtgccat cgcgagagcg cacagcacca ccacctgcag
     2401 tgcgacgatc ccgagcacgg ccaccaaccg gcccgccacc agcaaccagc gcggcaacgc
     2461 cgtggcggcg agccgccgga ccacgccgta gcggcggtcg aagcccagcg cgatggcctg
     2521 ccccgtgaag gcggtggaca tcacggccag cgcgagcacc ccggggacca ccgcggcgat
     2581 ccggggctcg ggcagcggca ccacgtcgag ggtgctcagc ccgaccagca gcacgatcgg
     2641 gatcagaagt gtgatcagca cctgctcgcc gttgcgcagg gccagccgca gctcggtgcc
     2701 cgactgggcg gcgagcatcc gcaatggcgc gccggcgcct ggctgcgggg tgaaggtgcc
     2761 tggtgcgaat cggttcacga gcgcagttcc cgtccg
//
LOCUS       DASRSC010000061         4363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_838730,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000061 DASRSC010000000
VERSION     DASRSC010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838730"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VFE39_03100"
     CDS             <1..511
                     /locus_tag="VFE39_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257618.1"
                     /translation="PGPSGQISLKAPARGATFSRSHTRARGHDTDPASQPQASCSRVS
                     QPIQYRHPDVHQNHVHRRTHDSQPPRPIDRLPAVGCRPAGSPLPGGWLTVLPGGRNIA
                     LGVLAVVAVLVRLLARPPVDVAGAARYAAAALTIATLLAQSTRVGRLVVPALLAGVAA
                     ALRPAHRRP"
     gene            564..1004
                     /gene="msrB"
                     /locus_tag="VFE39_03105"
     CDS             564..1004
                     /gene="msrB"
                     /locus_tag="VFE39_03105"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET6257619.1"
                     /translation="MDPVRADNSLAEPEPKVVKSDAEWREQLSPAEYQVLRKAGTERA
                     FTGEYTDTKTSGVYSCRACGAELFRSDTKFESHCGWPSFFTPLAGDAVIERVDTSLGM
                     KRVEVVCATCHSHLGHVFEGEGYATPTDLRYCINSVSLRLEPDA"
     gene            1152..2528
                     /locus_tag="VFE39_03110"
     CDS             1152..2528
                     /locus_tag="VFE39_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257620.1"
                     /translation="MPRRRYLVILLAGVLGLTATAGTAAGADPPDGGPDDGTARPGYR
                     IVLPDLAPLRVDGGETRVLTGVHRNAAYAIEIPAQWNGELVMWAHGFRGNGPELTVDP
                     PAYGLRERIAAQGYAWAASSYDRNGYDVASGVRSTSALAEEFGELVGVPERTYIAGVS
                     MGGHIVARSLEEYPGSYAGALPMCGVLGDQALFDYFLDFQVAAGALAGVPVYPPGPDY
                     ATAVLPRIYAGLGLAPNDPTVGTPAAQQLRAATVLDSGGPRPGADTSFAFWKDFLFGL
                     GVPDATPTPVDGLAADPALVAGNVGTDYAPDQPVDLDAAVQRVPVADERARESGRLTP
                     VAQVSGTPDAPVLSLHDLGDLFVPFVMEQVYATEVAEAGRSELLVQRAVRATGHCEFS
                     AAEAGRAWDDLVAWVKEGRQPAGDDVTDPATVADPTYGCRFSDPAAYAAAGAPSESDT
                     RRLFEPCA"
     gene            complement(2558..3253)
                     /gene="hemQ"
                     /locus_tag="VFE39_03115"
     CDS             complement(2558..3253)
                     /gene="hemQ"
                     /locus_tag="VFE39_03115"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HET6257621.1"
                     /translation="MARLDYAELNSTIRYTMWSVFRVEPGRLGEDRAEAATQLTEFLD
                     ALEGKGVAVRGVYDLAGMRADADFMIWWHAEHVEALQAAYSSLRRTTALGRASVPVWS
                     QVALHRPAEFNKSHLPAFIAGEEPKRYVSIYPFVRSLEWYLLPDEERRQMLADHGMQA
                     RGNPDVRANTVPAFALGDYEWILAFEADELDRIVDLMRHLRATEARRHVREEVPFFTG
                     PRVQPGDLMATLP"
     gene            complement(3407..>4363)
                     /locus_tag="VFE39_03120"
     CDS             complement(3407..>4363)
                     /locus_tag="VFE39_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6257622.1"
                     /translation="ADRLADPLLGGVYAGRVDELGLRATIPTLAAALDTGAPSLTAAA
                     DAATVPPPGAAPLDGRAPARPAPVFGALSGGYRTLLNALAAAARANVRLGTTVRELHR
                     RETGWRLTLGPTTAPESLDVDAVVLAIPAPAAARLLAAVAPAAARAAAEIELASSVVV
                     ALAYRGADASTLPPTSGALIATGEPLSVKAFTHSTNKWAHLAPDGLVRLRASLGRFGE
                     AATMQVDDTELVGRARADLAALTGITAEPIAVHVQRWGGGLPQYGVGHLDRVREIGDG
                     LPAGLAVAGSALHGVGVPACVGTGRGAAERIVAYLTAHAARA"
BASE COUNT          587 a         1630 c         1560 g          586 t
ORIGIN      
        1 cccaggtccg agcgggcaga tctcactcaa ggcacccgca cgcggggcca ccttttccag
       61 gagccacacc cgagccagag gtcacgacac cgaccctgcc agccagcccc aggccagctg
      121 ctctcgagtc tcacagccca tccagtaccg gcatccggat gtccatcaga accacgtcca
      181 ccggcgtacc catgactcgc agccgccccg cccgatcgac cgcctgcctg ccgtcggctg
      241 ccgcccggcc ggcagcccgc tgcccggcgg ctggctgacc gtgctgcccg gtggccggaa
      301 catcgcgctc ggcgtgctcg cggtcgtggc cgtgctcgtg cggctgctcg ccaggccacc
      361 cgtcgacgtg gccggcgcgg ccaggtacgc cgccgcggcg ctcaccatcg cgacgctgct
      421 cgcgcagtcg acccgggtgg gccggctggt cgtgccggca ctgctggccg gggttgccgc
      481 tgcccttcga cctgcccacc gtcgaccatg acgctgcgca gacggatctg cgccttgcgg
      541 gtcgcggaca tactctggaa cgcatggatc ccgtccgcgc tgacaactcg ctcgccgagc
      601 ccgaaccgaa ggtcgtcaag tccgatgccg agtggcgtga gcagctcagc ccggccgagt
      661 accaggtcct gcgcaaggcg ggcaccgagc gggcgttcac cggcgagtac accgacacga
      721 agacctcggg cgtctactcc tgccgcgcct gcggagccga gctgttccgc tccgacacca
      781 agttcgagtc gcactgtggc tggccgtcgt tcttcacccc gctggcgggc gacgccgtca
      841 tcgagcgggt cgacacgagc ctcggcatga agcgcgtcga ggtggtgtgc gccacctgcc
      901 acagccacct cgggcacgtc ttcgagggcg agggctacgc cacgccgacc gacctgcgct
      961 actgcatcaa ctcggtctcc ttgcggttgg agcccgacgc ctaggagcgc gcggaacagc
     1021 tcggccgtac tgcgcggcgc agctgttcag cacggaacgc gaggagatct cattcgcctc
     1081 ggctctggct tccggtccct gcgaccggta ggagtggtga tcgaccctgt cgccgtcgcc
     1141 ggaggttccc gatgccgcgc cgccgttacc tggtgatcct gctggccggc gtcctcgggc
     1201 tcaccgcgac ggcaggaacg gccgccggcg ccgaccctcc cgacggcggc ccggacgacg
     1261 gcacggcgcg gcccggctac aggatcgtgc tccccgacct cgccccgctc cgcgtggacg
     1321 ggggtgagac aagggtgctc accggggtcc atcggaacgc ggcctacgcg atcgagatcc
     1381 ccgcgcagtg gaacggcgag ctcgtcatgt gggcgcacgg tttccgcggc aacggcccgg
     1441 agctcaccgt cgacccgccc gcctacgggt tgcgcgagcg gatcgcggcg cagggctacg
     1501 cgtgggcggc gtcctcgtac gaccgcaacg gctacgacgt ggcctccggg gttcgctcca
     1561 cctcggcgct tgcagaagag ttcggcgagc tggtcggcgt cccggagcgg acgtacatcg
     1621 cgggtgtatc gatgggcggc cacatcgtcg cgcggtcact ggaggagtac ccgggctcct
     1681 acgccggcgc gctgccgatg tgcggcgtgc tgggcgacca ggcgctgttc gactacttcc
     1741 tcgacttcca ggtggcggcc ggtgccctcg ccggcgtgcc cgtgtaccca ccaggacccg
     1801 actacgccac cgcggtactg ccgcggatct acgccggcct cggcctcgcg ccgaacgacc
     1861 ccacggtcgg taccccggcg gcccagcagc tgcgagccgc caccgtgctc gactccggcg
     1921 gaccgcggcc cggcgccgac acctcgttcg ccttctggaa ggacttcctg ttcggactgg
     1981 gcgtgccgga cgcaaccccg actcccgttg acggcctggc cgccgacccg gcgctggtgg
     2041 cggggaacgt cggcaccgac tacgcccccg accagccggt cgacctcgac gccgccgtcc
     2101 agcgcgttcc cgtggccgac gagcgcgccc gggagtccgg ccggctcacc cccgtcgcgc
     2161 aggtatccgg gacgcccgac gcacccgtgc tgtcgctgca cgacctcggc gacctgttcg
     2221 tcccgttcgt catggagcag gtctacgcga ccgaggtggc cgaggccggc cgctccgagc
     2281 tcctcgtgca gcgggccgtc cgcgccaccg gtcactgcga gttctccgct gcagaggccg
     2341 ggagggcgtg ggacgacctg gtggcgtggg tgaaggaggg cagacagccc gccggcgacg
     2401 acgtgaccga tccggcgacc gtggccgacc ccacttacgg ctgccgcttc tccgacccgg
     2461 ccgcgtacgc cgccgccggc gcgccgtccg agagcgatac gcgtcgcctc ttcgagccct
     2521 gcgcctgacg atgccccgct gagcaggggc aggccgatca gggcagggtc gccatcaggt
     2581 cgcccggctg cacccgcggc ccggtgaaga agggcacctc ctcgcggacg tgccggcggg
     2641 cctccgtcgc ccgcaggtgc cgcatgaggt cgacgatccg gtcgagctca tcggcttcga
     2701 acgcgagaat ccactcgtag tcgcccagcg cgaaggccgg caccgtgttg gcccggacat
     2761 ccgggttacc gcgggcctgc atgccgtggt cggcgagcat ctggcgacgc tcctcgtcgg
     2821 gcagcaggta ccactccagt gaccgcacga aggggtagat ggagacataa cgcttgggct
     2881 cctcgcccgc gatgaacgcg ggcaggtggc tcttgttgaa ctccgcgggc cggtgcagcg
     2941 ccacctggct ccagaccggg acgctggcgc ggccgagggc ggtggtgcgg cgcaggctcg
     3001 agtacgcagc ctgcagcgcc tcgacgtgct cggcgtgcca ccagatcatg aagtcggcgt
     3061 cggcccgcat ccccgcgagg tcgtacacgc cgcgcacggc cacgcccttg ccctccagcg
     3121 cgtcgaggaa ctccgtgagc tgggtggcgg cctccgcgcg gtcctcaccc agcctccccg
     3181 gctcgacccg gaacacggac cacatggtgt accggatggt gctgttcagc tcggcgtagt
     3241 cgaggcgggc catggcatcc atcctgccac gtggaccgct cccgactgcc cggtggcgag
     3301 gcccgtcacg tgccggcgat cgccctgaac gaacggcccg tacgtccgac tgggccggac
     3361 gtacgggccc gttcgtgcga tgggagcacg ccccgtgggg cccgagtcac gcccgggcgg
     3421 cgtgggccgt gaggtaggcg acgatccgct cggcggcacc gcggcccgtg cccacacaag
     3481 ccgggacccc gacgccgtgc agagccgagc ctgcgacagc gagcccagca ggcagtccat
     3541 ccccgatctc ccgcacgcgg tcgaggtgac cgaccccgta ctgcggcagt ccgccacccc
     3601 agcgctggac gtggacggcg atcggctcgg ccgtgatccc ggtgagcgcc gccaggtcgg
     3661 ccctggcccg gccgacgagc tcggtgtcgt cgacctgcat ggtggcggcc tcgccgaagc
     3721 ggccgaggga ggcgcgcagc cggaccaggc cgtcgggagc caggtgagcc cacttgttcg
     3781 tcgagtgggt gaatgccttg accgacaggg gctccccggt cgcgatcagc gcacccgagg
     3841 tggggggcaa ggtgctcgcg tctgccccgc ggtaggcgag tgcgacgacg accgaggacg
     3901 cgagctcgat ctctgccgcc gcccgagccg cggccggcgc cacggcggcg agcagccgtg
     3961 ccgcggcggg agcggggatc gccagcacca cggcgtccac gtcgagcgac tcgggggcgg
     4021 tggtggggcc cagcgtgagc cgccagccgg tttcccggcg gtgcagttcc cggaccgtgg
     4081 tgccgagccg cacgttcgcc cgggccgcag cggcgagggc gttcaggagc gtccggtagc
     4141 cgccggacag ggcaccgaag accggtgcag gcctcgctgg agccctgccg tccagagggg
     4201 ccgcccccgg cggcgggacg gtagccgcgt cggcagccgc cgtcagggac ggggctccgg
     4261 tgtcgagggc ggcggcgagg gtggggatcg tggcgcgcag gcccagctcg tcgacccgcc
     4321 ccgcgtagac cccgccgagc agcgggtcgg cgaggcggtc ggc
//
LOCUS       DASRSC010000062         3270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_348048,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000062 DASRSC010000000
VERSION     DASRSC010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348048"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            389..919
                     /locus_tag="VFE39_03125"
     CDS             389..919
                     /locus_tag="VFE39_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257623.1"
                     /translation="MRTTVAAAPAGWRMRPRTRKGVLVVHIVAAGAWIGLDVMLGVLV
                     FTAMFTGDRATASLGYQALPLLLWPIIAAAVVCLASGVVLGLGTHHGLLRYWWVAVKL
                     ALNLLLTALVVLLLRPGLYEAAEYGRRLAAGPATADLGDLVFPPIVSSTALVVATVLS
                     VYKPWGRVRGSAAERA"
     gene            960..1727
                     /locus_tag="VFE39_03130"
     CDS             960..1727
                     /locus_tag="VFE39_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET6257624.1"
                     /translation="MRDYPQMLAATDHIEPVPRRVRAVLGGRTVFDTMRALYVWEWPS
                     YPQYYIPLSDVDPGVLVDEQHLQRLRRGAARRHGLRVGDIDRPGTARVFTDDALEGLA
                     GTVRFDWSAMDAWFEEDEEVFVHPRNPYARVDALRSTRTVRIELDGVVLAESASPVMV
                     FETGLPTRYYLYRTAVHFEHLERADTVTACPYKGRTSAYWSIRVGDQLYPDLAWAYDF
                     PTPQLLPIAGMVAFYNEKVDVILDGELVERPTTHFFR"
     gene            1940..2650
                     /locus_tag="VFE39_03135"
     CDS             1940..2650
                     /locus_tag="VFE39_03135"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HET6257625.1"
                     /translation="MPTTYQSSPATIARSTPQKDHRRTHLILMLALTFSTGVIDAVGY
                     LGLDRVFTANMTGNIVMLGMALAGSEHLPVARLALALVGFVVGAAVAGRWLRTAPAGW
                     SRRTTALLGAVAGVVLVMSLLLLRVGVHPADPVGSIITSILAVAMGVQAATARLVAVK
                     DVTTVVVTSTLTALAADSWPGGAAGLWRRRAPAVALMLLGAAAGTGLLRWNITPALLL
                     SSVVTAGVAVAGHRATRG"
     gene            complement(2764..2955)
                     /locus_tag="VFE39_03140"
                     /pseudo
     CDS             complement(2764..2955)
                     /locus_tag="VFE39_03140"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
BASE COUNT          432 a         1124 c         1199 g          515 t
ORIGIN      
        1 cgacgagtgt ctgctgacac cggatgacga cgtcgtgctg gcctcggggg acgagctcct
       61 cctggccggg cggcccgcgc ggcgcgcgct cagacgacga tggtcgtcga cgcgacccgg
      121 gagtacgtcg tgaccggccg gcacgtgccg gcgagctgga tctgggcacg actcttaccg
      181 gcgggcgacc gacggggagg ctgagcgccg cgtcgtgccg ccgtgccccg ggctgcgccg
      241 ggggcggtac gcatcgggtg atctgtaccg ctccgggtgc agggcgacgg acgcccggtg
      301 cgggatgtgc gaaccggaca tgatcgccac gattgagcgg tggccggcgg cacccgcggg
      361 ccgggctgtc cgggcaggag ggatcaggat gcggacgact gtcgcggcgg cgcctgccgg
      421 gtggcggatg cggccacgca ccaggaaggg cgtgctggtg gtccacatcg tggccgccgg
      481 agcgtggatc ggcctggacg tgatgctcgg cgtgctcgtc ttcacagcca tgttcaccgg
      541 cgaccgcgcc accgcgtcgc tgggctacca ggcactgccg ctcctgctgt ggccgatcat
      601 cgcggccgcc gtggtgtgcc tcgcaagcgg cgtcgtcctc gggctcggta cccaccacgg
      661 actgctccgg tactggtggg tggcggtcaa gctggcgctg aacctcctgc tcaccgcgct
      721 ggtcgtgctc ctgctgcggc cgggcctgta tgaggcggcg gagtacggca ggcggctcgc
      781 cgcggggcct gccacggccg atctcggcga cctggtcttc ccgccgatcg tgtcgtccac
      841 cgcgctcgtc gtcgcgacgg tgctgtcggt ctacaagccg tggggacgag tcaggggatc
      901 ggccgcggag cgggcctgat cctcggggtt atcgtgagga ctccgggaga ggaggcggga
      961 tgcgcgacta cccgcaaatg ctcgccgcga ctgatcacat cgagcccgtt ccgcggaggg
     1021 tccgcgcagt gctcggcggc cggacggtgt tcgacaccat gcgggcgctg tacgtgtggg
     1081 aatggccgag ctacccgcag tactacatcc cgctgtccga tgtggaccct ggcgtgctgg
     1141 tggacgagca gcacctccag cgcctgcgcc gcggtgccgc acggcgccac gggctgcgcg
     1201 tcggcgacat cgatcggccg ggaactgcac gcgtgttcac ggatgacgcc ctggaagggc
     1261 tggccggcac cgtgcgcttc gactggagcg cgatggatgc gtggttcgag gaggacgagg
     1321 aagtcttcgt gcatccccgc aacccctatg cccgggtcga cgcactgcgc tccacccgaa
     1381 ccgtgcggat cgagctcgac ggtgtggtcc tggcggagtc ggcctcgccg gtgatggtgt
     1441 tcgagaccgg tctgcccacc cgctactacc tttaccgcac cgcagtgcac ttcgagcacc
     1501 tggaacgggc cgacacggtc accgcatgcc cgtacaaggg caggaccagc gcgtactggt
     1561 cgatccgggt cggcgaccag ctgtatccgg acctggcctg ggcctatgac ttcccgaccc
     1621 cgcagctgct gcccatcgcc ggcatggtcg cgttctacaa cgagaaggtc gacgtgatcc
     1681 ttgacggtga gctcgtggag cggccgacaa cgcacttctt ccgctgacta cgacggcctc
     1741 gccgacaggg ccatacacga gggcggtcac agatgagagc gctctgcggt cctgcgtcgg
     1801 tagcgtgttt cgtgacgaga gcggcgccgt ctgccgcccc gcacgcgcaa cgccagcaca
     1861 ccattcgcgt ccgacctccc ccggcaccgc cgccctgtgg cggcccggag gcctgacgag
     1921 aacggacgcc ccccgcgcga tgcccacgac gtaccagtcc tcgcccgcca caatcgccag
     1981 aagtacgccc cagaaggacc atcggcgcac ccacctgatc ctgatgctgg cgctcacctt
     2041 ctccacgggt gtcatcgacg ccgtcgggta tctcggcctc gaccgtgtgt tcacggccaa
     2101 catgacgggg aacatcgtga tgctcggcat ggcgctggcg ggcagtgagc acttgcccgt
     2161 cgcccggctc gcgctggcgt tggtcgggtt cgtcgtcgga gcggcggttg ccggacggtg
     2221 gctgcgcacg gcaccggcgg gctggtcgag gcggacgacc gcgctcctcg gggccgtcgc
     2281 cggcgtcgtg ctggtgatgt cgctgctcct gcttcgggtc ggggtgcacc ccgccgaccc
     2341 ggtcggatcg atcatcacct cgatccttgc cgtggccatg ggcgtgcagg cggccaccgc
     2401 gcggctcgtc gcggtcaagg acgtcaccac ggtggtggtg acgtcgacgc tcacggcgct
     2461 ggcagcggac tcctggcccg gcggcgcggc agggctctgg cggcgccgcg cacccgcggt
     2521 cgcgctgatg ctgctgggtg ccgccgccgg taccggactg ctgcggtgga acatcacccc
     2581 ggcgctgctg ctgtcgtcgg tggtcaccgc gggcgtcgcg gtcgcgggcc accgcgctac
     2641 caggggctga gtcagcgccg gagccgcgac cgccactgcg agcacaccgt gcccccagcc
     2701 gaacgtcggc cggggcacgg ctctcgcgcc gctcaccggc ggtgctgccc ccgtgacccg
     2761 gtcgccgccg atctcggcgt tctgcacggg gacgaagctc tcccgggtgc cgaacagccc
     2821 ggtctgcaca gtgacccact tgggctggtc ggtgtcgtcg tcgtagtaga tctgaccgat
     2881 cttgccgatc ctgtcgccgg accggtcgta cgccgtcgac ccgatcaggt cgcgcaggtc
     2941 attcgcagaa gtcatcggtg ggttctcctc tgggggtggc ggtgtcccag gagccgccag
     3001 gcgccgtgat gcggaccggc ttgtggtgcg tctctcccac ggaccggccc ggactacccg
     3061 cccccaccgg gcgcacacga aaagcgttgc gcaaccgtgg cggatcccag tgtcggcgtg
     3121 ttcacccgaa ctgaccaggt gcgatgccgg cgatcggagg aagatcgggc gcggcgagga
     3181 gatgcggagg ttttgggggt gggggagccg tccgacgagg tcgacgacga gccgccggtg
     3241 ctgtgggccg cgggggcggt cgcgcgtcgg
//
LOCUS       DASRSC010000063         4675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5588885,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000063 DASRSC010000000
VERSION     DASRSC010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5588885"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..883)
                     /locus_tag="VFE39_03145"
     CDS             complement(266..883)
                     /locus_tag="VFE39_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257626.1"
                     /translation="MAARELHRLATGLLEGLGTEYWGFPPRLAPFIVEQLGPVRTVSW
                     FANNLPRYEHTLRTMGPLRTHLICMAISQQNGSRYSSYGHAYAAELIYFRKRGRLMPV
                     DARVLSDWIDLSPGELRDRLCNVLQRAELHVEVIWVDRTLALTSGDQWAVDQDEARIV
                     HLVRMFAVLNAISITGKVEPDEAHDPINKDTALKGRHAALRGTHA"
     gene            1234..2010
                     /locus_tag="VFE39_03150"
     CDS             1234..2010
                     /locus_tag="VFE39_03150"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6257627.1"
                     /translation="MPPDPALPDLSGAVVLVTGGGGGIGSGIAARFAAAGAAVVVHHR
                     DAGTERARVVAGAITARGGHAVTARADVTDPEQCASLMAVAVERFGRLDGVVAAAGVQ
                     PVVDLPDMSVDAWREVLDTNATSAFATVQAAAGVMDGHGGSITLIASIEGARPARAHA
                     HYAASKAATIMFARSAALEYGGAGIRVNTVSPGLVRRAGLAEAWPDGVGRWERAAPLA
                     RLGESRDVGDACVFLASPMARWITGHDLVVDGGMSVVPSW"
     gene            2004..2498
                     /locus_tag="VFE39_03155"
     CDS             2004..2498
                     /locus_tag="VFE39_03155"
                     /inference="COORDINATES: protein motif:HMM:NF017936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6257628.1"
                     /translation="MVSRAAGEQVAALLGLEPLPDEGGLFRRTHLDAHSSAIYYLLLA
                     PDFSALHKLGATETYHWYGGSPLRLLLLHPDGATDQPVLGPDLATGQRPQVVVPAGTW
                     QGSSPEGEWSLVGTTTAPPFDWAHFRLGDRADLVDRYPKTRMRIEELTRPVSRSAAPG
                     CSGR"
     gene            complement(2461..3246)
                     /locus_tag="VFE39_03160"
     CDS             complement(2461..3246)
                     /locus_tag="VFE39_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6257629.1"
                     /translation="MPTRDAPWPPGTPCWVDLAVPDVSAATDFYRSVIGWSFVDTGEE
                     FGHYHVARTNDRAAAAIGPVQQEGQPSAWTVYLATDDADATAKQIAANGGTVLVEPFD
                     IPGNGRMLVAFDTCGGVFGVWQAAGQNGVEIYNEPGSLVWTDARLTDPEAGTRFYAAV
                     FGYTFQPVPGAPDDYGTFQVDGEVAGGIGGMMGGPPDVPAHWLAYFAVADVDAAVDAA
                     ERGGATVVMRQQDSAFGRMSILVDPFGATFALNGPNSQEQQSG"
     gene            3395..>4675
                     /locus_tag="VFE39_03165"
     CDS             3395..>4675
                     /locus_tag="VFE39_03165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6257630.1"
                     /translation="MSEEAPVGRVVGTEDSTPLQFSVALAPEQYLQLDDVVVTVRPVP
                     GIGPVMTSGVVTEVRARHEGATFGSDVFLIADGVLPAQVQEIAEITTTRVEPECYVPP
                     TPGSLAKRATGEERARALYFDQMDLKVPVGLGRDGEPIYVNLEFLDGTRGGHVSISGI
                     SGVATKTSFALFLLYSIFRSGVLGRRSVNAKALVFSVKGEDLLFLDQPNNRLDDDLRA
                     AYAKLDLPATPFASAGFFAPPTPDDLSGRPHVTGRTSGVTAFWWTLAEFCAEELLPYV
                     FADAEDERNQYTMVIHQVAARLRREATPVGDGAIAVGGRVLRTYDDLVEFVSDRLTDE
                     DERRDWAGPVTGTGTINAFLRRLRSSLKPLRTIVRGDLTDAPGRRVSTDRHQVTVVDL
                     HNLPERAQRFVVGVVLASETARKEAAGPGGLLFTM"
BASE COUNT          691 a         1622 c         1640 g          722 t
ORIGIN      
        1 cactggtggc cctgtatgcg gaggatcccc gcctcggaca cgcacaggtg gagttcaccc
       61 cgtcgctgca gcgcctcgtc gaggtggtcc tgaccgggct cctcaagcgc gagcccggcg
      121 gagaatgtat tccgtagtcc cgcagtcgga aaccgccttt cggagaatca ttgcgatgat
      181 gaacgtggcg atcggaccgg cggtgacgat cactggcgtc agcacgaacg cgagtaccgg
      241 aatggaggtg ttccacaacg ccaggtcacg cgtgggttcc gcggagggcc gcgtgccgac
      301 ccttgagtgc cgtgtccttg ttgatcgggt catgggcctc gtccggctcg acctttcccg
      361 tgatgctgat ggcgttgagc acggcgaaca tgcgcacgag atgcacgatc cgcgcctcgt
      421 cctggtccac cgcccattgg tccccgctgg tcagggcgag tgtgcggtcg acccagatca
      481 cctcgacgtg caattcggca cgctgcagga cgttgcacag ccggtcgcgg agctcgccgg
      541 gagacagatc gatccagtcg gacaacaccc ttgcatccac cggcatgagc cgcccgcgct
      601 tgcggaagta gatcagctcg gccgcgtagg cgtggccgta tgagctgtac cgactcccgt
      661 tctgctggga gatggccatg cagatcaggt gtgttcgcag cgggcccatg gtgcgcaggg
      721 tgtgctcgta gcggggcagg ttgttggcga accagctcac cgtccggacc ggccccagct
      781 gctcgacgat gaacggcgca agtctgggtg gaaaccccca gtactccgtg cccagtcctt
      841 cgagaaggcc tgttgcgagc cgatggagct cgcgagcagc cattctccaa ccccctacct
      901 tgtgtagtga tgcaaatgct actcgcacgt gcagccgcat gtgcgtctga acagcggacg
      961 agttgtcgca cggatggatc ccgtcaatgg tatgtgacgt atccggccca ttaagagtag
     1021 tggtcgtcgc gctaaccggc tgatcggacg gtcgcgacac cccgcacttc ggccaatcgt
     1081 gcacatcgtt gtcgacacgg ttacaggctg ttacgacaat tgctgctgac gcgcgggcgg
     1141 cgcgtgtccg accgggcgtc acgttcggta ggagccgaaa gccggcgacg agcggcccga
     1201 gccccgttcg gcccaccggt gcacactcgc cgagtgccac cggatcccgc tctccccgat
     1261 ctgtccggcg cggttgtgct cgtcactggt ggaggcggcg ggatcggttc cgggatcgcg
     1321 gcacggttcg ccgcggccgg ggctgcggtc gtggtgcatc accgcgacgc gggcacggaa
     1381 cgagccaggg tggttgccgg cgcgatcacg gctcgcgggg gccacgcagt caccgcgagg
     1441 gccgacgtca ccgaccccga gcagtgcgcg tcgttgatgg cggtggccgt ggagcggttc
     1501 gggcggctcg acggcgtggt cgcggccgcc ggggtgcagc ccgtcgtcga tctcccggac
     1561 atgtccgtgg acgcctggcg cgaggtgctc gacaccaacg ccacgtcggc gttcgccacc
     1621 gttcaggcgg cggccggggt gatggacggg cacggcgggt cgatcacgtt gatcgcatcg
     1681 atcgagggtg cacgtccggc tcgggcccat gcacactatg cggcgtccaa ggcagccacg
     1741 atcatgttcg cgcgcagcgc cgccctggag tacggcgggg ccgggatccg ggtcaacacg
     1801 gtctccccgg gcttggtccg gcgtgccggg ctggccgaag cgtggcccga tggagtcggg
     1861 aggtgggagc gcgccgcacc cctggcgcgg ttgggcgaga gccgcgacgt cggcgacgcg
     1921 tgtgtgttcc tggcctcgcc gatggcgcgg tggatcaccg ggcacgatct cgtcgtcgac
     1981 ggcgggatgt cggtggtgcc gtcgtggtga gccgcgcggc aggcgagcag gtggccgccc
     2041 tgctcgggct cgagccgctg cccgacgagg gcggcctgtt ccggcgcacc cacctcgacg
     2101 cgcactcctc ggcgatctac tacctgctgc tcgccccgga cttctccgcc ctgcacaagc
     2161 tcggcgccac cgagacctac cactggtacg gcggatcgcc cctgcggctg ctcctgctgc
     2221 accccgacgg ggcgaccgac cagccggtgc tcggacccga tctcgcgacc gggcagcggc
     2281 cacaggtcgt ggtgccggcg gggacgtggc aggggtcgag cccggaaggg gagtggtcgc
     2341 tcgtcggcac gaccacggca cctccgttcg actgggcgca cttccggctc ggcgaccgcg
     2401 ccgacctcgt cgaccggtac ccgaagaccc ggatgcggat cgaggagctg acccgcccgg
     2461 tcagccgctc tgctgctcct ggctgttcgg gccgttgagg gcgaacgtgg cgccgaacgg
     2521 atcgacgagg atgctcatcc gcccgaacgc gctgtcttgc tgccgcatga cgacggtggc
     2581 accgccgcgc tcggcagcgt cgacggcggc gtcgacatcc gcaaccgcga aatacgccag
     2641 ccagtgcgca ggcacatcgg ggggaccccc catcattccg ccaataccgc cggcgacctc
     2701 gccgtcgacc tggaacgtgc cgtagtcgtc gggtgcgccg gggaccggct ggaaggtgta
     2761 gccgaagacg gcggcgtaga agcgcgtgcc ggcctcgggg tcggtgagcc gggcgtcggt
     2821 ccagacgagc gagcccggct cgttgtagat ctccacgccg ttctgcccgg cggcctgcca
     2881 gacgccgaag acgcctccgc aggtgtcgaa cgcgacgagc atccggccgt tgcccggaat
     2941 gtcgaacggt tcgacgagga cggtgccgcc gttggcggcg atctgcttag cggtggcgtc
     3001 ggcgtcgtcg gtcgccaggt agaccgtcca ggcggacggc tggccctcct gctgaacagg
     3061 cccgatggcg gccgcggccc ggtcgttggt gcgggcgacg tggtaatggc cgaactcctc
     3121 gccggtatcg acgaacgacc agccgatcac cgacctgtag aagtcggtgg ccgccgagac
     3181 atcggggacg gcgaggtcga cccaacacgg ggtgccgggg ggccagggag cgtcacgggt
     3241 gggcattgca cacacctcca tgggagtggg acggcgcaca gccaaccacc gaccaccgac
     3301 agaacgcgtc atccgatcgg ttgctcggcg ccccccgact ccccgtcccc cgcggccggt
     3361 tagggtctcc caccagcagt tgcgaggagg gaccgtgtcc gaggaagcgc cagtcggccg
     3421 cgtcgtcggc accgaggact ccacgcccct gcagttcagc gtggccctcg ctccagagca
     3481 gtacctgcag ctcgacgacg tcgtggtcac tgttcgtccg gtccccggga tcggtccggt
     3541 gatgacgtcc ggcgtggtca ccgaggtccg tgcccggcac gagggcgcca ccttcggctc
     3601 cgacgtcttc ctcatcgccg acggcgtgct gcccgcgcag gtgcaggaga tcgccgagat
     3661 cacgaccaca cgcgtcgagc ccgagtgcta cgtcccgccg acgcccggct cgctcgccaa
     3721 gcgggccacc ggcgaggaac gcgcgcgggc cctgtacttc gaccagatgg acctcaaggt
     3781 gcccgtcggg ctcggccgcg acggcgagcc gatctacgtc aacctggagt tcctcgacgg
     3841 cacccgcggc gggcacgtct cgatcagcgg catctccggc gtcgcgacga agacgagctt
     3901 cgcgctcttc ctgctgtact cgatcttccg cagcggcgtg ctcgggcgcc ggtcggtcaa
     3961 cgcgaaggcg ctggtgttca gcgtcaaggg cgaggacctg ctgttcctcg accagcccaa
     4021 taaccggctc gacgacgacc tgcgcgccgc atacgccaag ctcgacctgc ccgcgacgcc
     4081 gttcgcctcc gccggcttct tcgcccctcc cacgcccgac gacctctccg gccgcccgca
     4141 cgtcaccggc cggacgagcg gtgtcaccgc gttctggtgg acgctcgccg agttctgcgc
     4201 cgaggagttg ctgccctacg tcttcgccga cgcggaggac gagcgcaacc agtacacgat
     4261 ggtgatccac caggtcgccg cgcggctgcg gcgggaggcc acacccgtcg gcgacggtgc
     4321 gatcgctgtg ggcggccggg tgctgcgcac ctacgacgac ctcgtggagt tcgtctccga
     4381 ccggctcacc gacgaggacg agcgccgcga ctgggcgggt ccggtcacgg gcaccggcac
     4441 gatcaacgcc ttcctgcggc ggctgcgctc cagcctgaag ccgctgcgca cgatcgtccg
     4501 cggcgacctc accgacgcac cgggacgccg cgtgagcacc gacagacacc aggtcacggt
     4561 cgtcgacctg cacaacctcc ccgagcgcgc ccagcggttc gtcgtcggcg tggtgctggc
     4621 atccgagact gcccgcaagg aggccgcagg gcccggcggg ctgttgttca cgatg
//
LOCUS       DASRSC010000064         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2967675,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000064 DASRSC010000000
VERSION     DASRSC010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2967675"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(540..836)
                     /locus_tag="VFE39_03170"
     CDS             complement(540..836)
                     /locus_tag="VFE39_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257631.1"
                     /translation="MTLWMAELPDGRQVIGEAADEAEARRMTGQEQSREVVSWHPERQ
                     QWRWTLVVDGGNTHHGWAETRAKAQLAIALAEHPDPVAHRMVVGRLRWSLSKEE"
     gene            959..1159
                     /locus_tag="VFE39_03175"
     CDS             959..1159
                     /locus_tag="VFE39_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6257632.1"
                     /translation="MPPRERRPRLLLTSELAEELGVSARLIQQYRHDGILTPTMETPK
                     GHARWDLEDVRKQLRALREKNG"
     gene            complement(1665..2222)
                     /locus_tag="VFE39_03180"
     CDS             complement(1665..2222)
                     /locus_tag="VFE39_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6257633.1"
                     /translation="MAERPLRADARRNRERVLAAADEAFADHGFGVPLDEIAAAAGVG
                     PGTVYRHFPTKEALFEAVSLARVQDVVDDARARADAADPGSAFEGFLARLAEEAGRKR
                     DLPDALAGAGAHGIAGAVAELHDALGVLLARGQAAGAVRGDITVPDLVALLKGLLHTV
                     GTDPEPGLRQRVFAVLRDGLRPPVE"
     gene            2363..2800
                     /locus_tag="VFE39_03185"
     CDS             2363..2800
                     /locus_tag="VFE39_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6257634.1"
                     /translation="MSRSPREVVEQVRRMVAGEGVAFADLFAADGVLTYPFAVPGMPR
                     ELRGRDAILAYHGAASEQRRMLEIEGVDVLVRETDDPEVVVAEIEHHGHSHGTGGPYR
                     FRALGVIRVRDGEIVAYDDYMDPIAMAALLGRTPDLVAALSAA"
     gene            complement(2725..>3231)
                     /locus_tag="VFE39_03190"
     CDS             complement(2725..>3231)
                     /locus_tag="VFE39_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257635.1"
                     /translation="CSRNARWARCGVTCGGAGVHRSMFPLLWAASAAAIEQRRSAAWW
                     RWVPTWPVYAVTAIGVLGVLHVLPITPISAHADQPLQIGNFQLDEIGWPQMVEDVAAA
                     YRALPAETVRNAIVVTEDYWTVSAIQHFAPDLPSYSTCRPRVQAAESAATRSGVRPSS
                     AAMAMGSM"
BASE COUNT          509 a         1183 c         1085 g          454 t
ORIGIN      
        1 gcgtccggac gttccagcgc gagagcgcct gagcagccgg tgcgccggtg acggctcccg
       61 cggatcggac tcgtcgcctg cgcggcgggc ggggtggaga cggtccgggc ggacttcgtg
      121 gagcccgccc tgacccgtcc ggggctgggg cgtggcgatc accctcagcc ccactgcttc
      181 ccgtccgcga ccaggtgagg cgtgcgggcg ggtcacggcc ccacccaccg ctcgactgct
      241 acctcgtcgc accggccacg gccatcacgg tcgcgaagct caccctcgga atcgcggact
      301 accaaaccct caccacagcg ggcaactcgt gtccggcgag gacgtgtggc cgctctccga
      361 gccgccagtg accggccgct gccgtgatcg gcgatcctcg aactggtcga cgagatcacc
      421 aaccggtagc ggtcgcccga tccgtatgac ccggacactt ttccgggctg tcctgtcgtc
      481 cccgcccccg ccgggcgaag gatctccacg gcgcgcacca caatgagcgg ggtagaagct
      541 cactcctcct tcgacaggga ccaacgcagc cgacctacga ccatccggtg agctaccgga
      601 tctgggtgct cggcgagtgc gatggccagc tgcgccttcg cccgggtctc ggcccagcca
      661 tggtgggtgt tgccgccgtc cacgacgagc gtccatctcc actgctgccg ctccgggtgc
      721 cacgacacca cctcgcggga ctgctcctgc ccggtcatcc gccgcgcctc cgcctcgtcc
      781 gctgcctcac cgatcacctg tcggccgtcc ggtagctccg ccatccacaa cgtcacgtga
      841 cgagcatgac gaaagtcccg caaagtctgc aaaatctgca agatacgcag atcatgcaac
      901 agacgcagaa gccgcttagc gcgtggtacg ttgctcggtc cgctcctacg gtgccaaggt
      961 gcccccgagg gaacgacggc ccaggctgct cctgacgagc gagctagcag aggaacttgg
     1021 ggtgtccgca cgcctgatcc agcagtaccg gcacgacggc atcctcacgc ccacgatgga
     1081 gaccccgaag gggcatgctc ggtgggatct ggaggacgtc cgcaagcagc tccgcgcact
     1141 ccgcgagaag aacggctgac cttcgggcat gagccctgtc ggcatgcgac cctgagcatg
     1201 ttgccgcgtc ccccggccgt cgatctgcat cgatggaggg aagccgcgta cgagtcatgg
     1261 acgtccacca ccaggccagc caccacggcc gctagatcgg cccgtgtgcg tcgacctgga
     1321 agctgtacgg gccatcggac gagacgccgg gcgctcgaaa ctgagcgccc gcccagacct
     1381 acgcgatctt ggatgccgct cgtatcgcga tacgggagca gtcgtgcgat ccgccgggga
     1441 accacaggct cgccagtcgc cctcgaagcg ccctgcaatg ggctcccctg actgcgccag
     1501 ggggatccac ccgagctcaa gcgcagaccg gcccgccgcc gccgtcgatc aacggtgggc
     1561 cggtcgcgca gtgcggtgcc ccccatccgg gtcccccgac ccggccgcac acatacgacg
     1621 ttagggcaga aggacgcgaa gcggatcagt cgaaaggttg atcctcactc gaccggcggt
     1681 cggagcccgt cgcgcagcac cgcgaacacg cgttgccgta gacccggctc cgggtcggta
     1741 ccaacggtgt gcagcagccc cttgagcagc gcgaccagat ccggaacggt gatgtcaccg
     1801 cggacggccc cggccgcctg cccgcgcgcc agcagcaccc ccagtgcgtc gtgcaactcg
     1861 gcgaccgcac cggcgatgcc gtgcgcgcct gccccggcga gcgcgtcagg caggtcacgc
     1921 ttgcgtcccg cctcctcggc cagccgggcg aggaaaccct cgaacgcaga ccccgggtcc
     1981 gctgcgtctg cacgcgcccg cgcatcgtcg acgacgtcct gcacgcgggc cagcgagacc
     2041 gcctcgaaca gcgcctcctt cgtcgggaaa tgccggtaga ccgtgccggg accgacaccg
     2101 gccgcggcag cgatctcgtc gagcggaacg ccgaacccgt gatccgcgaa cgcctcgtcg
     2161 gcggcagcca gcacgcgctc ccggttgcgg cgcgcgtcgg cacgcagagg ccgctcagcc
     2221 atggtggccc gttcgcaccc agcggtgacc ctcggcccga cgcgggcgcc tccccgtccc
     2281 gcggatcaac tcgcagttgc taagcggggc ggtcgttccg tatcgttgaa ccggggtcac
     2341 cgttccgatt ctaggaggat tcatgagccg cagtccgcga gaggtcgtcg agcaggtccg
     2401 tcgcatggtg gccggggaag gtgtcgcctt cgccgacctc ttcgcggccg acggcgtgct
     2461 cacctatccc ttcgccgtgc cggggatgcc gcgggagctg cggggccggg acgccatcct
     2521 ggcttaccac ggcgccgcct ccgagcagcg gcggatgctg gagatcgagg gcgttgacgt
     2581 tctggtccgc gagactgacg accccgaggt cgtcgttgcc gagatcgagc accatgggca
     2641 ctcgcacgga accggcgggc cctaccggtt ccgcgcgctg ggcgtgatcc gggtgcggga
     2701 cggcgagatc gtcgcctacg acgactacat ggaccccatc gccatggccg cgctgctcgg
     2761 ccgcaccccg gacctggtgg cagcgctctc ggcggcttga acgcggggac ggcaggtgct
     2821 gtatgagggg aggtcgggcg cgaagtgctg gatcgccgac accgtccagt agtcctccgt
     2881 gacgacgatg gcgttgcgca cggtctccgc cggcagcgcg cggtaggcgg cagccacgtc
     2941 ctccaccatc tggggccagc cgatctcgtc gagctggaag ttgccgatct gcaggggctg
     3001 gtcggcgtgc gcggagatcg gcgtgatcgg cagcacgtgg aggacgccga ggacgccgat
     3061 cgccgtcacc gcgtagacgg gccaggtcgg cacccagcgc caccatgccg ccgacctgcg
     3121 ctgctcgatc gcggccgccg atgcagccca gagcaggggg aacatcgatc gatggacccc
     3181 ggctccaccg cacgtcacgc cgcagcgcgc ccaccgggcg ttgcgcgagc a
//
LOCUS       DASRSC010000065        13890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3133186,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000065 DASRSC010000000
VERSION     DASRSC010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 13890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13890
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3133186"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VFE39_03195"
     CDS             complement(<1..226)
                     /locus_tag="VFE39_03195"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HET6257636.1"
                     /translation="MTTTTTTRLAIGLVGLGHMGGAMADRLLAAGHVVYGTSHTRERA
                     ETLIAHGLRWMDTPHEVGRAADVIITSIPDD"
     gene            complement(229..1194)
                     /locus_tag="VFE39_03200"
     CDS             complement(229..1194)
                     /locus_tag="VFE39_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6257637.1"
                     /translation="MNETPVETTPLGKTGLDITRVGFGAWAIGGGGWEFGWGPQDDDE
                     SIAAIHRALDLGVNWIDTAAAYGFGRSEQMVGRALDGLTGDRRPYVFTKASLLEGPGR
                     VVVHSLDRDSILREADASLNRLGIDAIDLYQVHWPIPAEDIEEGWAAFAELKEQGIVR
                     HIGVSNFTVEQLRRAQQIAPVETLQPQYSLIDREAETQLLPVAEREAIGVLVYSPMAS
                     GLLSGAMTRDRITHLADDDWRKRDPRFTEPRLSQHLAQVERLRAVADRRSTTPGAVAI
                     AWALRNPAVDGAIVGFRRPGQVDPLVSAANLRLDAADISTIEGQD"
     gene            complement(1327..1962)
                     /locus_tag="VFE39_03205"
     CDS             complement(1327..1962)
                     /locus_tag="VFE39_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6257638.1"
                     /translation="MVADDDVLLREGLSSLLAGSGFEVVGQAGEPDELLALVREHRPD
                     LVVVDIRMPPTHTTEGLAAAQVIRAELPQTAILVLSAHVEVEQATELLASGQRSGYLL
                     KDRIGDVDDFLGTLDRIIKGAAVVDPALVQELLAARRVDDPLQVLSHREREVLALMAE
                     GRSNAGIAHPLWVAEGTVEKHVRSILMKLRIPETSDDHRRVLAVLTFLDSL"
     gene            2365..2769
                     /locus_tag="VFE39_03210"
     CDS             2365..2769
                     /locus_tag="VFE39_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6257639.1"
                     /translation="MALRCLIVDDSPGFLEVARTVLEREGMEIVGVASTGSEALQSAA
                     DLHPDVTLLDIDLGAESGFVVARWLAADPDLDPGALILISAHAQEDIADLLDASPAVG
                     FLSKPALSATAIERLVRAAGGGHNRPGESLRS"
     gene            2995..5169
                     /locus_tag="VFE39_03215"
     CDS             2995..5169
                     /locus_tag="VFE39_03215"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET6257640.1"
                     /translation="MATQWSDGLVAEQDALRRVATLVATGAPPEAVFATVAEEVGRLL
                     EVDTTILSRHESDGTQVSVGAWSNTDVAVPFPVGTRVRLGGRNVVSMVVRTGRPARIE
                     DYADATGSVAHAARGWGLRCAVGVPIGVEGRLWGVISVASGRARSIRADTEARLAAFT
                     ELVATSIANAQARVELRRFAEEQAALRRVATLVARAAPPEEVFAAVTAEVGRVLVADI
                     VVLCRYEADGVETVVGVWSSADDPPVAVGTRTRLDGRNVSTRVLRTGRPARIDDYSDA
                     TGPVGVFAHEVGIRASVGVPIRVDGGLWGVMIVATERTSPPEDSEVQLAGFTHLVGTA
                     IANAQARTELRRSAEEQAALRRVATLVARAAPPEEVFAAVTAEVGQVLSVAFTVLSRY
                     ETDGAATVLGTWTSAGGAFPVSIGTRVELGGRDVHTLVSRTCRPARLEHYDQDVGAAT
                     ATVLTAGVRASVGAPISVGGRLWGVVIVAPETDQLLSPDTETRLAGFTELIATALANV
                     EAQAALTASRVRLVAAADAARRRIERDLHDGAQQRLASLALELGAVRAVVPREADELA
                     AYVDGVATELTGLLADLSEIARGLHPAALAEGGVRPALKALARRSVVPVRLHLPTEGR
                     YPDQVEIAVYYVVAEALTNVAKHAHANLVEVAVMAGDGSMVVSVRDDGRGGADITGGT
                     GLVGLTDRVEALGGRLRVQIAPGTGTAVQAVIPLSPEPAVSG"
     gene            5265..7505
                     /locus_tag="VFE39_03220"
     CDS             5265..7505
                     /locus_tag="VFE39_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET6257641.1"
                     /translation="MATGPSDVVAEEQAALRRVAMLVARAASPEELFGAVAAEVGLLM
                     GVDFTILSRYDPDDTAVTVAAWARTGSEDPIDFGTRWAVGGDNVTTRVFTTGRPARID
                     VYALASGPAAEVGRRLLFRSAVGAPIIVAGRLWGLMLVAYRHEQWLPPDTEVRLARFT
                     ELVATAIADAQSRVELRGYAEEQAALRRVATLVADGAPSMQVFTALAGEVGHLLGCSF
                     TVLNRYDPDGTAVTVGAWAGTGDMSFPVGTRWSRGGHNVTTLVSETGRPARIDGYAGT
                     TGPAADLARAAGACSIVGVPIFVEGRLWGFMSAASTREEVLAADTETRLAGFTELAGT
                     AIANAQARLELRGFAEEQAALRRVATLVAQAAPPEEVFFAVAAEVGLVLRVDLTILSR
                     YDSAKSATVVGVWSGSGDTASAPVGSRFELGGENVHTEVFRTRRPARVNHAEASGPAT
                     SVFRPLQIRSAVGVPISVEGGLWGAMLVLSTREEFLPTNIEVRLAAFTELVGTTVANA
                     DAQAALTASRARIVATADTTRRRIERDLHDGAQQRLVSLHLHLRGAVLAAVPPGADEL
                     AGRLAAIGTGLVGVLEELREIARGVHPASLTAGGLRPALKTLARRSAVPVRLDVRVDG
                     RLPECIELAAYHAVAEALTNVVKHAHATVVDVHGEIVERRLNVRVRDDGRGGADATAG
                     SGLVALTDRVEALGGRFWLSSVPGAGTTMEISLPLTGLDGWGAECGSPRGSGADRARS
                     VDARRK"
     gene            complement(7580..8578)
                     /locus_tag="VFE39_03225"
     CDS             complement(7580..8578)
                     /locus_tag="VFE39_03225"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6257642.1"
                     /translation="MTTRCRRRSAPRHTDPALLRTAGPAGGALLAGRYRLAERVGRGA
                     SADVWRARDELLDREVAVKQFHEPHTHSLIEARLAARVRHPNVAAVHDVVGHGSAYSL
                     VMDYCGGTNLVALLRGEGRLPESVVAMLGLQLLAALQAVHASGVVHCDVKPANLMLDD
                     DGRLVLIDFGIAEINGSDPAHPARRSGDVIGSPAYMAPELVLGGAPRPASDLWSLAAT
                     LYTAVEGIPPFRQDDDVPTLTAVLHDPPSPTRRAGRLQPVLDRLLVKEPADRASPDVV
                     HALLTDACPTTPPVLTAQRTAPDRDGAEPTISTPIPRLLGPSWGNVGWGRRLIGTA"
     gene            9123..10514
                     /locus_tag="VFE39_03230"
     CDS             9123..10514
                     /locus_tag="VFE39_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6257643.1"
                     /translation="MILLDVSIVNVALPSIERDLGVSAGSVQWVLSGYALTLGLALVP
                     AGRLGDTLGRRRMFLIALSAFVVTSALTGAAPTIGLLIAARLVQGVAGGILVPQNTGL
                     IQELFRGAERGRAFGVLGGTVGLATAAGPVIGGLILAAFTGPDGWRWVFYVNVPIGLL
                     ALVLAARLVPSTRVGRVRDIRLDLVGTLLLGGGVLSLLLPLVDSEAVGLAEWYLFVVA
                     VLLLGAFAWWERRTVRRGRQPLLDPRLARTPGYAAGSGIALVYFAGFTGIGLVFALFF
                     QDGLGYSPLHSGLAVTPFALGVAASAVIAGRLVSRIGRWLTVSGLATVVVGLVATALV
                     LRHVDGSAAAWAAAGPLLLAGLGGGMVTSPNITLSLESVAVEMAGAAGGALQTAQRIG
                     SAIGTAALVAIFYEALVRNGRDYPAAVYDALLCASGVMLLALLMAVAELVRRHHHDRL
                     LPVSRPEHDAHRV"
     gene            10996..11514
                     /locus_tag="VFE39_03235"
     CDS             10996..11514
                     /locus_tag="VFE39_03235"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6257644.1"
                     /translation="MVLVHGFAGSSAIWFAIRRALCAAGRVLVTLNDPPWASSMDRLA
                     DRLIDAVEDLLAVTGAGKVRLVGHSLGGLVIALALTGRRLAGRVDLEVTLGTPFGGSP
                     WAGMVPVCLIARVALPPDRSERCGRALILLRRLAAALTADWCDGSRSPRRLTRSSLLV
                     APDPPTGRPRAS"
     gene            11511..11639
                     /locus_tag="VFE39_03240"
     CDS             11511..11639
                     /locus_tag="VFE39_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257645.1"
                     /translation="MIDADGHPGMMLDPDVAVRIAAAPSLPEDAAEAADLDDPVNA"
     gene            complement(11712..12980)
                     /locus_tag="VFE39_03245"
     CDS             complement(11712..12980)
                     /locus_tag="VFE39_03245"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET6257646.1"
                     /translation="MSLLAVGLTHRGAPLAALERTAVGADDLPKLLDELLARDDVTEA
                     LLLSTCNRVEVYADVDTVHGGLADISAVLARHGGWDVDELAGQLLVHHEGSAVEHLFL
                     VAAGMDSVVVGEPQILGQLRAAYATASDLGTVGRVLHDLVQHALRVGKRAHAETGIDA
                     AGASVVSEALAAGARVLGGLRGRRAVIVGAGSLAGLTAAELRRAGVAEIVVANRTSGT
                     ATRLAATVALSGTPARAVGVDALPTELAADIVITCTGSFDTVLTTSMVAAAGPTVICD
                     LALPRDVEPAVRTLPGVTLIDLEDLADRLRRAEAGTGVEAARTLVAAEVRRYLAAQRS
                     ASVDPTVTALRRRAAEVVEAELVRLGARLPGLPTEAGSELSRTVHRVVDTLLHTPTVR
                     VKQLAEGPTGTRYAEALRVLFALDPQPALR"
     gene            complement(13155..>13890)
                     /locus_tag="VFE39_03250"
     CDS             complement(13155..>13890)
                     /locus_tag="VFE39_03250"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HET6257647.1"
                     /translation="AIERGEYPSWTLKVQIMPYEDAKTYRFNPFDLTKVWPHGDYPLQ
                     EVGRLTLDRNVTDHHSEIEQAAFAPSNQVPGTGLSPDKMLLARGFSYADAHRARIGVN
                     YKQIPVNAPRSDVHNYSKDGRMRVHTATEPFYAPNSKGGPQADPARAAEVHWEADGDM
                     VRSAYTLREDDDDYGQAGALVREVLDDAARDRLVGNVVGHVLNGVSEPVLTRVFEYWR
                     NIDKTVGDRIEQGVRTDGADLGQPKA"
BASE COUNT         1761 a         4797 c         5147 g         2185 t
ORIGIN      
        1 cgtcgtcggg gatggaggtg atgatgacgt cggcggctcg gccgacttcg tgtggcgtgt
       61 ccatccagcg gaggccgtgc gctatgagcg tctccgcgcg ctcgcgggtg tggctggtcc
      121 cgtacaccac gtgtccggcg gcgagcaggc gatcggccat ggccccgccc atgtggccga
      181 gtccgacgag cccgatcgcg agtctggtgg tggttgtcgt cgtcatggtc agtcctgccc
      241 ctcgatcgtg ctgatgtcgg ctgcgtcgag ccggagattc gcggcgctga cgagcggatc
      301 gacctggccc gggcggcgga agccgacgat ggcaccgtcg accgccgggt tgcgcagggc
      361 ccatgcgatc gccactgcgc ccggggtggt ggaccgacgg tcggcgaccg cccggagccg
      421 ctccacctgg gcgaggtgct gcgacagccg cggctcggtg aagcggggat cgcgcttgcg
      481 ccagtcgtca tcggcgaggt gcgtgatgcg gtcgcgggtc atggccccgc tcagcaggcc
      541 cgaggccatg ggggagtaca cgaggacacc gatcgcctcg cgttccgcga cggggaggag
      601 ttgggtttcc gcctcgcggt cgatcagtga gtactgcggt tgcagcgtct cgacaggggc
      661 gatctgctgt gcgcgtcgta gctgctcgac ggtgaagttc gagacgccga tgtggcggac
      721 gatgccctgt tccttcagct cggcgaatgc cgcccagccc tcctcgatgt cctcggcggg
      781 gatcggccag tggacctggt agaggtcgat ggcgtcgatc ccgagccggt tcaggctcgc
      841 gtcggcctcc cgcaggatcg agtcacggtc gaggctgtgc accacgaccc gcccggggcc
      901 ctcgaggagc gaagccttcg tgaagacgta cgggcgacgg tcgccggtga ggccgtccag
      961 tgcgcgaccg accatctgtt cggagcgtcc gaagccgtag gccgcggcgg tgtcgatcca
     1021 gttgacaccg aggtcgagcg cgcggtggat cgcggcgatg gattcgtcgt cgtcctgggg
     1081 accccatccg aactcccagc cgccgcctcc gatcgcccag gcgccgaagc ccacgcgggt
     1141 gatgtcgagg ccggtcttgc ccaacggggt ggtctccacg ggggtctcgt tcatggctcc
     1201 agcctcgccg cggcggtgga cgacgtcgcc ggtgccacca cgagtttccg ttaccggctt
     1261 gcccgaggcc ccgggtaccg gccagccgga gttgcagccg ggaacgccgg cggggacacg
     1321 cctcgatcag aggctgtcga ggaaggtgag tacggccaga acgcgacggt ggtcgtcgga
     1381 tgtctcgggg atgcgcagtt tcatgaggat gctgcggacg tgtttctcga ccgttccctc
     1441 cgccacccac agcgggtggg cgatgccggc gttggaccgg ccctcggcca tcagggccag
     1501 cacctcgcgt tcgcggtggg agaggacctg caaggggtcg tccacccggc gggcggcgag
     1561 cagttcctgg acgagcgccg gatcgacgac cgcggctccc ttgatgatcc ggtccagggt
     1621 gccgaggaaa tcgtcgacgt cgccgatccg gtccttgagc aggtagccgc tgcgctgccc
     1681 gctggcgagc agttcggtgg cttgctcgac ctcgacgtgc gcggacagga ccaggatcgc
     1741 ggtctgcggg agttcggcgc ggatcacctg ggctgcggcg agcccttcgg tggtgtgggt
     1801 cgggggcatc cggatgtcca cgacgaccag gtcgggtcga tgctcgcgga ccagcgcgag
     1861 cagttcgtcg ggttcaccgg cctgaccgac gacctcgaat cccgacccgg ccaggagact
     1921 gctcaggcct tcgcgcagca ggacgtcgtc atcggcgacc atgacccgac cgcggtttcc
     1981 cgccacggga tcgagtatca ggcccgcggc cggccctgcc cagccctcgg ggggtgccga
     2041 ccgacggtcc gtcatcggtc ctccagctga gccgcccacg gtgccctccc ggcgtgccgc
     2101 tcgttcggac catcacggcc ccgatcgcgc cggccgagga cgtgtggaca atccccggcc
     2161 gggccctgtc agcctcgttc cggggagtgc gggtgtcatc gcggcctccg gcaacccgtg
     2221 atggtcgcca gccggagtcg tcgtctaccg gccagccgga ggggtcgccg gctgcgcgag
     2281 cccgagggcg cccctcagga cacggcgcgg cggatggtgg ctacctgcaa tggctcgccg
     2341 acgccgcact cctagccttg cctcgtggcc ttgcgctgct tgatcgtcga cgacagtccg
     2401 ggctttctcg aggttgcccg tacggtgctc gaacgggagg ggatggagat cgtcggagtc
     2461 gcgtcgaccg gttccgaggc tctccagagc gcagccgatc tgcatcccga cgtgacgctg
     2521 ctggacatcg atctgggggc cgagagcggc tttgtcgtgg cgcggtggct ggccgccgat
     2581 cccgacctcg accccggggc cctgatcctg atctcggctc acgcacagga ggacatcgcc
     2641 gacctcctcg acgcgagtcc ggccgtcggg ttcctgtcca agcctgcgct gtccgcgacg
     2701 gcgatcgaga ggttggtccg cgcggctggt ggaggtcaca accggcctgg tgagtcgctt
     2761 cgctcctgag cccgacggac gggagatggc ggcggggtgc agcgggcgga ggtccgcggg
     2821 cgcctcggcg tcttcgccca cgggtcttcc gagagatgtc ttgacctgct tcgatcggtg
     2881 tcaggaccgg ccttctgggg cggggcgtat tcctttccgc gcccgccgcc gcgggcacgc
     2941 gggcggggcg ccccgccgag gtgatggtgt ggcgtccgcc ggagtggagg cccgatggcc
     3001 acccagtgga gcgatgggct cgtcgcggaa caggacgcgc tacgccgcgt ggccacgctc
     3061 gtcgcgacag gggcaccacc ggaggcggtc ttcgccacgg tggccgagga ggtcggccgg
     3121 ctgctggagg tcgacaccac gatcctgagc cgtcacgagt cggacggtac gcaggtgtcc
     3181 gtgggtgcgt ggagcaatac ggacgtcgcc gtcccgtttc ccgtcgggac tcgagtgcgt
     3241 ctgggcggcc ggaacgtcgt gtcgatggtc gtccggacgg gccgcccggc gcgcatcgag
     3301 gactacgccg acgccaccgg atccgtggcc catgcggctc gcggctgggg gctccgctgc
     3361 gccgtcggcg tgccgatcgg tgtcgagggc cggctgtggg gggtcatcag cgtggcctcc
     3421 ggacgagcac ggtcgatcag ggccgacacc gaggcacggc ttgcggcgtt caccgagctg
     3481 gtcgcgacct ccatcgccaa cgcccaggcc cgtgtggagc tgcgccggtt cgccgaggag
     3541 caggccgcgt tgcgccgggt ggcgacgctc gtggcccggg ccgcgccacc ggaggaagtg
     3601 ttcgccgcgg tcaccgccga ggtgggccgg gtgctcgtcg ccgacatcgt cgtcctgtgc
     3661 cgctatgagg ccgacggtgt cgagactgtc gtcggcgtgt ggagcagcgc cgatgacccg
     3721 cccgtcgcgg tcggtacccg cacgcgcctc gacgggcgga acgtgagcac ccgggtgttg
     3781 cgcaccggcc ggccggcacg gatcgacgac tactcggacg ccaccgggcc cgtcggtgtc
     3841 ttcgctcacg aggtcggcat ccgggcgtcg gtcggggtgc cgatcagggt ggacggcggg
     3901 ctgtggggcg tcatgatcgt ggcgaccgag cgcacgtcac cgccggagga ctcggaggtg
     3961 cagctggccg ggttcactca cctggtcggg acggcgatcg ccaacgccca ggcccgcacc
     4021 gagctgcgcc ggtccgccga ggagcaggcc gcgttgcgcc gggtggcgac gctggtggcc
     4081 cgggccgcgc caccggaaga ggtgttcgcc gcggtcaccg ccgaggtggg tcaggtgctc
     4141 tccgtcgcct tcacggtctt gagcaggtac gagacggacg gtgcggccac ggtcctcggc
     4201 acctggacca gcgctggcgg cgcctttccg gtctcaatcg gcacccgggt ggagctcggt
     4261 ggccgggacg tgcacaccct cgtgtcgcgg acctgccggc ctgcgcggtt ggagcactac
     4321 gaccaggatg tgggcgccgc aaccgccacc gtgctgaccg ctggggtgcg ggcctcggtc
     4381 ggcgcgccga tcagcgtcgg gggccgactg tggggcgtcg tgatcgtggc acccgagacc
     4441 gaccagctgc tgtcgccgga cacggagacg cggctggccg gcttcaccga gctgatcgcc
     4501 acggctctcg ccaacgtcga ggcgcaggct gcgctgaccg catcaagggt gcggctggtg
     4561 gccgccgccg acgccgcccg ccgccggata gaacgcgacc tgcacgacgg cgcgcagcag
     4621 cggctcgcct ccctcgcgct ggagctgggc gccgtgcggg ctgtggtgcc gcgcgaggcc
     4681 gacgagctgg ccgcgtacgt ggacggggtc gccacggagc tcaccgggct gctcgccgac
     4741 ctgagcgaga tcgcccgcgg actgcatccc gccgctctcg ccgagggagg tgtgcgcccg
     4801 gccctgaagg cgctggcccg ccgctcggtc gttccggtcc ggctgcacct gccgaccgag
     4861 gggcgatatc cggatcaggt cgagatcgcg gtgtactacg tggtcgccga ggcactgacc
     4921 aatgtggcca agcacgcgca cgccaatctc gtcgaggtgg ccgtgatggc gggcgacggc
     4981 tccatggtgg tcagcgtccg cgatgacggc cgcggtggtg cggacatcac ggggggaacc
     5041 ggtctggtcg ggctcacgga tcgagtcgag gcgctcggtg gtcggctccg ggtgcagatc
     5101 gcacccggta ccggcaccgc cgtgcaggcc gtgatcccgc tgtccccgga gcccgccgtc
     5161 tccgggtgat ccaggcggac ggtccttgat gggccgaccc gcggcgagca cgctgtcgga
     5221 ccaatgaggt gaaggcgtcg gggagcgccg gggcggagac ccagatggct accggaccga
     5281 gcgacgttgt cgccgaggag caggcggccc tgcgacgggt ggcgatgctg gtggcccggg
     5341 cggcgtcgcc ggaggagctt ttcggcgcgg tcgccgccga ggtcggtctg ctgatgggag
     5401 tcgacttcac gatcctgagc cggtacgacc cggacgacac ggcggtcacg gtcgcggcgt
     5461 gggctcgcac cgggagcgag gaccccatcg acttcggcac ccgatgggcc gtcggtgggg
     5521 acaacgtgac tacccgggtg ttcaccacgg gtcgaccggc gcgcatcgac gtctacgccc
     5581 tcgcctctgg cccggccgcc gaggtcggac gccggctgtt gttccgctcg gccgtcggtg
     5641 cgcccatcat cgtcgcgggc cggctgtggg gactcatgct ggtggcgtac cggcacgagc
     5701 agtggctgcc gccggacacc gaggtgcggc tggcccggtt caccgagctc gtcgccacgg
     5761 ccatcgccga cgcgcagtcc cgcgtcgagc tgcgtgggta tgccgaggag caggcggcgc
     5821 tgcgccgggt cgcgacgctg gtcgcggacg gagcgccatc gatgcaggtg ttcacggctc
     5881 ttgccgggga ggtcgggcac ctgttgggct gcagtttcac cgtcctcaac cgctacgacc
     5941 cggacgggac ggcggtcacc gtcggtgcgt gggccggcac cggcgacatg tcctttcccg
     6001 tgggcacccg gtggagccgt ggtgggcaca acgtgaccac cctggtgtcc gagacgggcc
     6061 gcccggcccg gatcgatggc tatgccggca ctacgggccc agccgccgac ctcgcgcgtg
     6121 cggcgggggc ctgctccatc gtcggcgtgc cgatcttcgt ggaggggcgg ctgtgggggt
     6181 tcatgagcgc ggcgtccacc cgcgaggagg tcctggcggc ggacaccgag acgcggctgg
     6241 ccgggttcac cgagctggcg gggacggcga tcgcgaacgc gcaggcgcgc ctcgagctgc
     6301 gcgggttcgc cgaggagcag gctgcgctgc ggcgggtggc gacgctggtg gcgcaggccg
     6361 cccccccgga ggaggtcttc ttcgccgtcg cggcggaggt cgggttggtg ctccgtgtag
     6421 acctcaccat cctgagccgc tacgactcgg cgaaatcggc caccgtcgtg ggcgtgtgga
     6481 gcggctccgg cgatacggca tcagcccccg tcggcagccg gttcgagctc ggcggtgaga
     6541 acgtgcacac cgaggtgttc cggacccggc gtccggcgcg cgtgaaccac gccgaagcct
     6601 ccgggcccgc gaccagcgtg ttccgaccac tccagattcg ctcggcggtc ggggtgccga
     6661 tcagcgtcga gggcggcctg tggggtgcca tgctcgtgct gtccacccgc gaggagttcc
     6721 tgccgacgaa catcgaggta cggctggcgg cgttcaccga gctggtcggc acgaccgtgg
     6781 ccaacgccga cgctcaggcc gcgttgactg cctcccgggc gcgcatcgtc gccacggccg
     6841 acacgacacg ccggcgcatc gagcgcgatc tgcacgacgg ggcccaacaa cggctggtct
     6901 ccctgcacct gcacctgcgc ggtgcggtgc tcgcggcggt gccgcccggg gccgatgagc
     6961 tggcagggcg actcgccgcg atcggtaccg ggttggtcgg tgtgctcgag gagctgcgcg
     7021 agatcgcccg cggcgtacac cccgcatcgc tcactgccgg cgggctgcgc ccggcgttga
     7081 agacgttggc ccggcgatct gccgtcccgg tccgcctcga cgtccgcgtc gacggacggc
     7141 tgcccgaatg tatcgagctc gccgcatacc acgccgtcgc cgaggccctc accaacgtgg
     7201 tgaagcacgc gcacgccacc gtcgtcgatg tccacgggga gatcgtcgag cgccggctga
     7261 acgtccgggt ccgcgacgac ggtcgcggcg gcgccgacgc cacggcgggc tccggccttg
     7321 tcgcgctcac cgatcgggtc gaggcgctcg gtggccggtt ctggctctcc agcgtgcccg
     7381 gtgcgggcac caccatggag atctccctgc ccctcaccgg actcgacggc tggggagcgg
     7441 aatgcgggtc cccccgcggc tcgggggcgg atagggcgcg atccgtggat gcccgtcgga
     7501 agtgatctct cgcagttcga gacccgcgat cgtgctcatc gcccgtggcg ccacccgtct
     7561 cgccggcccc ttcgcgtcgt caggcggtgc cgatgaggcg ccgtccccac cccacgttgc
     7621 cccaggaggg gccgagcaga cgggggatcg gcgtgctgat cgtcggctct gccccgtctc
     7681 gatccggagc agtgcgctgc gcggtcagga ccggaggcgt ggtggggcag gcgtcggtga
     7741 gcagcgcgtg gacgacgtcg ggggaagctc ggtccgcggg ttccttgacc aggagtcggt
     7801 ccaggaccgg ctggagacga cccgcccgcc gagtcggtga cggtgggtcg tgcagcacgg
     7861 cggtgagggt cgggacgtcg tcgtcctgcc tgaagggcgg gattccttcg actgccgtgt
     7921 agagagtcgc ggcgagcgac cacaggtcgg acgccggacg cggagccccc ccgaggacga
     7981 gctcgggggc catgtacgcc ggggagccga tcacgtcacc gctccggcgg gcggggtggg
     8041 cggggtcgct gccattgatc tcggcgatac cgaagtcgat cagcaccagt ctgccgtcgt
     8101 cgtcgagcat gagattcgcc ggcttcacgt cgcagtgcac gacaccggac gcgtgcactg
     8161 cctgcagtgc agcgagcagc tggagaccaa gcatggcgac gaccgactct ggcagtcggc
     8221 cctcaccccg gagcagcgcc acgaggttcg tgccgccgca ataatccatc accaggctat
     8281 acgcgctgcc gtgccccacc acgtcgtgca cggcagccac attggggtgt cgcacccggg
     8341 cggcgagccg cgcttcgatg aggctgtgcg tatgcggctc gtggaactgc ttgacggcaa
     8401 cctccctgtc caggagctcg tcgcgtgccc gccacacgtc ggcgctcgcc ccgcggccca
     8461 cccgctcggc cagccggtag cgaccggcca gcagcgcacc gcctgccgga ccagcggtcc
     8521 gcagcagggc tggatccgta tgtcgaggcg ccgatctgcg gcggcaacga gttgtcacga
     8581 tggctttcct tccgatcgca ggccggcgtc ttctgccgcg aacctctgcg agctctcgag
     8641 ggtgtccgcg gctggtcgcc cgaggcgttg aacacacgac gatccccggt ccgtcgctcg
     8701 ctgcctgccc ggtcagccgc caacggcggc ccagccagcc accgtcgatg agcccccgct
     8761 agctgatagt gcggtcggcc aactcaccgt gggcatcgcg ggagacgggc tgcgcgtcgt
     8821 ccccgcgccc cgctcgtcct ccccagccag cgggaatcgg gcactccggc cagccaccgt
     8881 gacgacggcc gctggcagtg atggcacgac tccggaatcg cgcgacgttg tcgacgcgcc
     8941 ccgccatccc ggcgtcggct ccgtcgccgc ccaccgagag gaacaccgcg atgaagaacg
     9001 ccgtaggagg acgggttcgc gaggagaccc cgtcccccgg ccatacgacg tcgccggcgt
     9061 ctgatcaggc aagcgggcga cgccgatggt gggcgctggg ggtcactcag ctcgctgcgt
     9121 tcatgatcct tcttgacgtc agcatcgtga acgtggcact cccgtccatc gagcgggacc
     9181 tgggcgtgtc ggcagggagc gtgcagtggg tgttgtccgg gtacgcgctc accctggggc
     9241 tcgcgctggt accagccggc cggctcggcg atacgctggg ccggcgccgc atgttcctaa
     9301 tcgccctgtc cgccttcgtg gtgaccagcg cgctgaccgg tgctgcgccg accatcgggt
     9361 tactgatcgc agcgcggctc gtgcaaggcg tggcgggcgg catcctggtg ccgcagaaca
     9421 ccgggttgat ccaggagctc ttccgtggcg ccgagcgagg cagggcgttc ggggtcctcg
     9481 gcggcaccgt cgggctcgcg acggcggcgg gtccggtgat cggcgggctg atcctggctg
     9541 cgttcaccgg cccggacggc tggcgttggg tgttctacgt caacgtgccg atcggcctgc
     9601 tggccctggt gctcgcggca cgactcgtgc cgtccaccag ggtcggcagg gtgcgcgaca
     9661 tccgcctgga cctggtcggg accctgctgc ttggtggtgg cgtcctgtcc ctgctgctgc
     9721 ccctcgtcga cagcgaggcc gtcggccttg ctgagtggta cctgttcgtg gtcgccgtgc
     9781 tgctgttggg tgccttcgcg tggtgggaac ggcgaacggt gcgccgagga cgccagccgt
     9841 tgctcgaccc acggttggcc cgcacgcccg gatacgccgc cgggtcgggc atcgccctgg
     9901 tctacttcgc cggcttcacg gggatcgggc tggtgttcgc gctgttcttc caggacggac
     9961 tgggctattc gccgctgcac tccggtctgg ccgtgacgcc gttcgccctc ggagtggccg
    10021 cctctgcagt gatcgcgggc cggctggtgt cccggatcgg gcgttggctg actgtgtcgg
    10081 ggctggccac tgtggtggtc gggctggtcg ccacggcgct cgtgctgcga catgttgatg
    10141 gatccgcggc ggcgtgggcc gccgcggggc cgctgctgct ggccggactc gggggcggca
    10201 tggtgacctc gcccaacatc acgctgagcc tggagtcggt cgcggtggag atggcgggcg
    10261 cggcgggtgg tgcgcttcag accgcccagc gaatcggctc ggccatcggg actgctgcgc
    10321 tcgtcgcgat cttctatgag gccctagtcc gcaatggtcg ggactacccg gcggcggtgt
    10381 acgacgcgct gctgtgcgcc tccggcgtga tgctcttggc gctcctgatg gcagtcgcgg
    10441 agttggtccg ccgtcaccac cacgaccggc ttctaccggt ctcgcgaccg gaacacgacg
    10501 ctcaccgggt ctgacggtgg acaaccgacg gccctgatga cctgtagtcg cgccggtcga
    10561 gatatgtccg ccagcaggca ccctggtctc tccctgcccg ttgcccccaa gttggcctct
    10621 cgtggtgatg ctccatcccc ggcgccgatg cgtgtgtccg gatagggacc atgcaaggag
    10681 ccggtcatga caccgtccgt gccgctccgc ctcgtcgcag cctccagtgg cgatcgcctc
    10741 tcgagccagg gtggtgcggc ggccaggccc ttgtcgggca gacgagtgac gcgccttcgg
    10801 gtgcatgctc gagggccggc gacgcgatcc aatcagaagg tggcggtcgg cgcgggccgt
    10861 gacgtccacg gtgacggctg ctggtgtgta cggctttccc cccgggtctc acccgtcgca
    10921 ccgcgcgtga ggtcggtcga cgacgcggca gtcctcggct gctcccgacc aagcggcgaa
    10981 cgaataccat cgcccgtggt gctggtgcac ggcttcgccg gctccagcgc gatctggttc
    11041 gccatccgcc gggccctctg cgccgctggg cgcgtgctcg tgacgctcaa cgacccgccg
    11101 tgggcgtcgt cgatggacag gctcgccgac cggctcatcg atgccgtgga ggatctgctc
    11161 gccgtgaccg gcgcgggcaa ggtgcgcctc gtcgggcaca gtctgggcgg gttggtcatc
    11221 gctctggcac tgacgggccg ccgcctcgcc gggcgcgtcg acctggaggt cacgctcggt
    11281 acgccgttcg gtggctcacc ctgggccggc atggtccctg tgtgcctcat cgccagggtc
    11341 gctttgccgc ctgatcggtc cgagcgctgc ggccgggctc ttatcctgct gcgacgcctg
    11401 gccgcggccc tgactgccga ttggtgcgat ggctcgcgtt cgcctcggcg gttgacgcgg
    11461 tcgtccctgc tcgtcgcgcc ggacccgccc accggcaggc cacgcgcgtc atgatcgacg
    11521 ctgacggcca ccccggcatg atgctggacc ccgatgtcgc cgtgcggatc gccgcagcgc
    11581 cttcgctccc agaggacgct gctgaggccg ccgatctcga cgatcccgtc aatgcctgac
    11641 ccgacggcgt gggtgggtcg aatcccctcc caccgacggc tcctgttccg gctctgcggc
    11701 tactgctgta ttcagcgcag cgcgggctgc gggtcgaggg cgaacagcac gcgcagcgcc
    11761 tccgcgtagc gggtccctgt cggtccctcc gcgagctgct tcacgcgaac tgtcggggtg
    11821 tgcagcaacg tgtccacgac ccggtgcacc gtccgggaga gctccgaacc cgcctcggtg
    11881 ggcagccccg gcagccgggc gccgagccgg accagctcgg cctcgacgac ctctgccgca
    11941 cgccggcgca gcgcggtgac tgtggggtcg accgacgcgg accgttgcgc ggcgaggtag
    12001 cggcgcacct ccgcggcaac cagcgtgcgc gccgcttcga cgccggtacc tgcctcggcc
    12061 cggcgcaggc ggtcggccag gtcctcgaga tcgatgaggg tgaccccggg cagcgtccgc
    12121 acagcgggct cgacgtcgcg gggcagcgcc aggtcgcaga tcacggtcgg cccggcggca
    12181 gcgaccatcg acgtcgtcaa caccgtgtcg aacgacccgg tgcacgtgat cacgatatcg
    12241 gccgccagct cggtcggtag cgcgtcgacc cccacggccc gcgccggcgt gcccgagagg
    12301 gccacggttg cggccagtcg ggtcgccgtt cccgatgtcc gattggcgac gacgatctcg
    12361 gcgaccccgg ctcggcgcag ctcggccgcg gtgaggccgg ctagcgaccc ggcaccgacg
    12421 atcacggctc tgcgcccccg cagaccgccg aggacgcgcg ccccggcggc gagcgcctcg
    12481 gacaccacgg aggcgccggc ggcgtcgatg cccgtctccg catgcgccct cttcccgacc
    12541 cgcaaggcgt gctggaccag atcgtgcagc acccgaccca ccgttccgag gtcgctcgcc
    12601 gtcgcgtagg cggctcggag ctgcccgagg atctgcggct caccgacgac caccgagtcc
    12661 attccggccg ccaccaggaa caggtgctcg acggccgatc cctcgtggtg cacgaggagc
    12721 tgaccggcaa gttcgtcgac atcccagccg ccgtggcggg cgagcacggc ggagatgtcg
    12781 gccagaccgc cgtggacggt gtccacgtct gcataaacct cgacccggtt gcaggtggag
    12841 agcagcagtg cctcggtcac gtcatctcgg gcgagcaact cgtcgagcag cttgggcagg
    12901 tcgtcggccc ccacggcggt ccgctccaga gcagcgaggg gtgcgccgcg gtgcgtaagg
    12961 ccgaccgcga gcaggctcat gtgctgatca cctttcctca aagcccgcgc cggacggggc
    13021 ctgagggccc gcgagcgcgg gagatggcaa gtgccatggc ggggagggcc cggcgatccg
    13081 ctgctcggat cgccgggcgt cggatcacac ccctcgaact gaccgttcgt cccgggtgcg
    13141 tgtccgatcg gggtctacgc cttcggctgc cccaggtcgg ccccgtcggt tcggacgccc
    13201 tgctcgatcc ggtcgccgac ggtcttgtcg atgttgcgcc agtactcgaa gacgcgagtg
    13261 agcaccggct cactcacgcc gttgagcacg tgtccgacga cgttgcccac gagtcggtca
    13321 cgggcggcgt cgtcgagcac ctcgcgcacc agggcaccgg cctggccgta gtcgtcgtcg
    13381 tcctcccgca gggtgtaggc ggaacggacc atgtcgccgt cggcctccca gtgcacctcg
    13441 gcggcgcgtg ccgggtcggc ctgcgggccg cccttggagt tgggcgcgta gaacggctcg
    13501 gtcgcggtgt gcacccgcat ccggccgtcc ttcgagtagt tgtgcacgtc ggacctgggc
    13561 gcgttgaccg ggatctgctt gtagttgacg ccgatccggg cgcgatgggc gtcggcgtag
    13621 gagaacccgc gggccagcag catcttgtcc ggtgagagcc cggtgccggg cacctggttg
    13681 ctgggtgcga aggcggcctg ctcgatctcg ctgtggtggt cggtgacgtt gcggtccagc
    13741 gtcagccgtc cgacctcctg cagcgggtag tcgccgtgcg gccagacctt ggtcaggtcg
    13801 aacgggttga accggtaggt cttggcgtcc tcgtacggca tgatctgcac cttgagcgtc
    13861 cagctcgggt actccccgcg ctcgatcgcg
//
LOCUS       DASRSC010000066         9749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2806054,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000066 DASRSC010000000
VERSION     DASRSC010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9749
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2806054"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..687)
                     /locus_tag="VFE39_03255"
     CDS             complement(4..687)
                     /locus_tag="VFE39_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6257648.1"
                     /translation="MLPTRVFVPPNGHVLLALPPTAASASVLRSLRSEGVSVSPVSDG
                     IGVLDAVRSRDPALLVLDVELPGPDQAAVLAAVQAEAPNVPIIAVTPRDRRASLLGQL
                     RGDRDDYLLRPFAVDELAARIRLRLRLGAAIENSVIRHGELTVDTEIGEVGIDGQTVG
                     LSPTEYAMLMALLAAPGEIVSPDRLARDVWSESASANLVQVYISYLRRKIGPERIRTV
                     RGEGYVLEA"
     gene            1070..2092
                     /locus_tag="VFE39_03260"
     CDS             1070..2092
                     /locus_tag="VFE39_03260"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET6257649.1"
                     /translation="MTIGRHRRPGGDVNAAVVTTDPLPQRSMTRRLAPLAVGAAVMLI
                     ATTVATVVRPPEDAAVAAPELSTAIVRTDPTTAVVTPAPAPAPPLDSTTALAAPADHA
                     APHSSAAAKAPKPARKPAAPVPVPKKPAGDGKQAAAVHGWTQVGGDEFNGGMSSKWTA
                     YEGPGHAGNGTRTPNAISVENGSLVIRGDSKGNTGGMAWSEGQRFGKWEMRAKFPAGD
                     SQYHPVLILWPTDVDWPQGGEVDFAETNSASKDVSFFLHYSSSNQQKYATKNIDITQW
                     HNYAVEWVDGRITGYIDGEKWFESTDGSTMPPGKMHPTIQLDYFPDGGSPKSSEMLVD
                     YMRIYR"
     gene            complement(2343..2558)
                     /locus_tag="VFE39_03265"
     CDS             complement(2343..2558)
                     /locus_tag="VFE39_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257650.1"
                     /translation="MSQPKPSRPEQPEPGAERELADAEPVSEPEPLNRAARRAKAKQT
                     DPSHVGPRGGLNRQVRGARSHTKRRTS"
     gene            complement(2938..4971)
                     /locus_tag="VFE39_03270"
     CDS             complement(2938..4971)
                     /locus_tag="VFE39_03270"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HET6257651.1"
                     /translation="MLALVSANPFLAPSPLPFEFPDFDAIREEHFAPAFTTGMEQQRA
                     EVDSITADSAAATFDNTIVALERSGAVLRRVSAVFFTLVGSCSTPGVRAIEAEIAPQL
                     AAHSDAIMLDSALFARIETLFATRNDLGLDAESLRLLERRHRDAVRAGARLGAAEQER
                     LRALNAELSTLSTQFGAKLLAGANDAAVLVEDPAELAGLSPDAVSAAARAATARGHDG
                     AHVISLVLPTGQPVLASITDRALRERVHRASVTRGLGGEHDTREIVLRMAALRAERAA
                     LLGHPHHASWVVEIGTAGTVDAIDAMLGKMAPVAAANAQAEADELSAAAGHPIEAWDR
                     AFHAERVRRERFDLDADMLRPYLELERVLHDGVFHAAGRLYGLRFSERHDLPRYHPEV
                     RVFDVFDENGQLGLFVADLYARESKRGGAWMNSFVTQSRLVGTKPVVLNTLNLARPAD
                     GQPTLLTIDNVRTLFHEFGHALHGLFSDVQYPTFAGTAVPRDFVEYPSQVNEMWLEDP
                     EVLANYARHHETGEPLPGELVARLADSRQFGEGFATTEYLAAALLDQAWHRLGRGDEV
                     TDVEQFEGEAIAAAGVAVPTAPARYRSTYFNHIFGGGYSAGYYSYIWSEVLDADTVEW
                     FAENGGLRRENGETFRRELLSRGGSVDPMEAYRAFRGRDAEIAPLLARRGLAG"
     gene            4978..5394
                     /locus_tag="VFE39_03275"
     CDS             4978..5394
                     /locus_tag="VFE39_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6257652.1"
                     /translation="MTQVRVGVGAVVVDDGRILVLRRAGAHGAGTWGLPGGHQEFGES
                     PERTAVREVAEETGLVVEATARLGFTDDPMPEIGRHYVTLFIACTSTGGELRLMESDK
                     ATELAWLTPDDLLARRDELFVPLRHCLDAGLLDVKR"
     gene            complement(5509..6954)
                     /gene="mftG"
                     /locus_tag="VFE39_03280"
     CDS             complement(5509..6954)
                     /gene="mftG"
                     /locus_tag="VFE39_03280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system GMC family oxidoreductase
                     MftG"
                     /protein_id="HET6257653.1"
                     /translation="MPRQEFDVVVIGAGSAGCALAGRLAEDPAKQVLLLEAGPAETMP
                     DVLDAASLAATRAGHPSNWAYAAQLRPGRTATVPRGRGLGGSSAINGAIWARATPADA
                     DGWTVPGWTWADLLPHYIRSEHDLDHPATPWHGDRGPVPVRRPAGGLLHPAATRFLDA
                     AERIGFPAEPDKNAGVPPGAGLVPSNSVDGVRVSAAHAYLDRHRANLVVRPRAGVARV
                     RFDGDRTRGVELLDGTVLEAGEVVLAAGAIATPHLLLLSGIGPPDDLRAAGLPVVCGL
                     PGVGRGWSDHPAVFLPFSTADPPAHPHAPGCQAALNLDAGADPAGDVEVLVFVRPFIT
                     DADLHLMCMLQQPDSRGTITLTSPDPRIRPRISYAYLRTEHDRRRLRHAIRTAAELLR
                     AGLGTRTAPTGEVLGSDRALDGWIAAHLSTAQHLSGSAAMGAVVDAELRVHGVRGLRV
                     ADTSVLPVVPRRGTAATAVAIGEKAATLLRS"
     gene            6974..8245
                     /locus_tag="VFE39_03285"
     CDS             6974..8245
                     /locus_tag="VFE39_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HET6257654.1"
                     /translation="MSNLAQDLADFISAAPTPYHAVAEGVRRLTAAGFAEQPEAGPWD
                     DGPGGRYLVRDGTLLAWFVPEEAPPGTPVRVFAAHTDSPTLKVKPRPDVGAGGWRQIA
                     VEVYGGALWNSWLDRDLGLAGRLALYDGTTVLVDVHRPLLRVPQLAIHLDRQVNQGLK
                     LDAQEHLLPVWGLGSPSDGDLIEFLADEVGVEPDEVAAHDLVVHDVTPPALLGEEEEL
                     LAAPRLDNLASVHAGLGALLAAADDVSVSGQAVIPVFVGFDHEEIGSGSASGAAGPLL
                     ETVLTRLAGGFDARGAAFAGSRCLSVDVTHAAHPNYLGHYDPTHRALPNRGPALKVNA
                     NQRYATDAPGAAAWHRACRDADVPTQVFVGRNTLPCGSTVGPLLATRLGIRTVDVGIP
                     VLSMHSARELCGVRDPDHLAAAATAFLTDPM"
     gene            complement(8229..8513)
                     /locus_tag="VFE39_03290"
     CDS             complement(8229..8513)
                     /locus_tag="VFE39_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257655.1"
                     /translation="MDEPIIESLDVQGARLTYDRRGFVRTPLVGPVDDDRRLADVHMP
                     YRPNLVLAERLGLEVVDLAGDHVGYAMHPEEFASQLGALLSGAGTVTSGR"
     gene            8704..9063
                     /locus_tag="VFE39_03295"
     CDS             8704..9063
                     /locus_tag="VFE39_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HET6257656.1"
                     /translation="MFDLARDGHTDVLAAHIDAGVPANLTNDKGDTLLILAAYHGHAE
                     TVAALLARGADHARVNDRGQTALAAATFHRSADAVTALLDAGADPEQGSQSALATAMF
                     FELPEMLELLRREEGPS"
     gene            9060..9344
                     /locus_tag="VFE39_03300"
     CDS             9060..9344
                     /locus_tag="VFE39_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257657.1"
                     /translation="MTAEEASLTRGRDVYWFVGGPLDGRVDTRAPSEPAPCTVRYVHL
                     HDGPKIVHHYDLHQVAGHGGEYRFRYSSADESDDPMSDELISGDAEDDTS"
     gene            9490..>9749
                     /locus_tag="VFE39_03305"
     CDS             9490..>9749
                     /locus_tag="VFE39_03305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HET6257658.1"
                     /translation="MTGELQDDGLFGPDSVTWRVHLEPVLWVGGFRALLLQSLHPRVM
                     RGTYQNSALFDPRKAWSRFQRTVEFVGVRTFGSTPAVEAAAAR"
BASE COUNT         1412 a         3523 c         3518 g         1296 t
ORIGIN      
        1 gtgtcaagcc tcgaggacgt aaccctcgcc acgcaccgtc ctgattcgtt ccggaccgat
       61 cttgcgccgg agataggaga tgtagacctg cacgaggttg gccgacgccg actcggacca
      121 gacgtcgcgc gcgagccgat cgggcgacac gatctcaccc ggggcggcga gcagtgccat
      181 cagcatcgcg tactcggtgg gtgacaaccc gaccgtttgc ccgtcgatgc cgacctcacc
      241 gatctcggtg tcgactgtca gttcgccgtg gcggatcacg ctgttctcga tcgcggcccc
      301 gagtcgcagc cggagcctga tccgcgcggc gagctcgtcg acggcgaagg ggcgcaggag
      361 atagtcgtcg cggtcgccgc gcagctgccc gagcaagctc gcgcggcggt cccgaggagt
      421 gacagcgatg atcggcacgt tcggcgcctc ggcctggacg gcggccagca cggcggcctg
      481 atccgggcca ggcagctcga cgtcgagcac cagcagcgcg gggtcgcgtg agcggacggc
      541 gtcgagcacg ccgataccgt cgctgaccgg gctgacggag accccctccg aacggaggct
      601 gcgcagcacg gaggcggacg cggccgtcgg aggcagcgcc aagaggacat gaccgttcgg
      661 gggtacgaac acacgtgtcg gcagcactgc cgagacgtac gaggacgcac cccccggggg
      721 agcagcgggg agcatcgccg cccagcatct cgcagctgcg cacggataac aatcgtctaa
      781 cggagcaacg gccagccgct cgcagatcac ggtccgtgtt cactgagagt cccagtactc
      841 caatcggagt acggaagcag gcctgtgtgc cggtttgagg tcgttctggc gcgcctgacc
      901 gacctgttgg cgcctacgcg caacctccgc tttaaggttt gctgatcacc tggcgagacc
      961 cgccttattc ttccgcctca tgcgacggac atcctccgct gcggaagatc gctcggaaac
     1021 cccgaccgag ctgatcgcac gtatttccgc agagcacggc tcccccaccg tgacgatcgg
     1081 acggcaccgc cgaccgggcg gcgacgtcaa cgctgccgtg gtcaccaccg acccgctccc
     1141 ccagcgctcc atgacgcgca ggctcgcccc gcttgccgtc ggcgccgccg tgatgctcat
     1201 cgcaacgaca gtcgcgaccg tcgtgcggcc cccggaggac gccgcagtcg cagcccccga
     1261 gctgagcacc gcgatcgtgc gcacggaccc gacgaccgct gtcgtcaccc ccgcccccgc
     1321 ccccgctccg cccctcgact ccaccaccgc cctggcggcg cccgccgacc acgccgctcc
     1381 ccattcttca gccgcggcga aggcccccaa gccggcgcgg aagccggcgg ctccggtgcc
     1441 cgttcccaag aagcccgcag gcgacggcaa gcaggccgca gcggtgcacg gctggaccca
     1501 ggtcgggggc gacgagttca acggcggcat gagctccaag tggaccgcct acgaagggcc
     1561 ggggcacgcg ggcaacggca cccggacgcc caacgcgatc agcgtcgaga acggctccct
     1621 ggtcatccgg ggcgactcca agggcaacac cggcggcatg gcgtggagcg agggccagcg
     1681 cttcggcaag tgggagatgc gcgccaagtt ccccgccggc gactcccagt accaccccgt
     1741 gctgatcctc tggccgaccg acgtcgactg gccgcaaggc ggcgaggtcg acttcgccga
     1801 gaccaacagc gcctccaagg acgtgtcgtt cttcctgcac tacagctcga gcaaccagca
     1861 gaagtacgcg acgaagaaca tcgacatcac ccagtggcac aactacgcgg tggagtgggt
     1921 cgacggccgc atcaccggct acatcgacgg tgagaagtgg ttcgagtcca ccgacggcag
     1981 cacgatgccg cccggcaaga tgcaccccac gatccagctc gactacttcc ctgacggcgg
     2041 ctcccccaag tcgtccgaga tgctggtcga ctacatgcgg atctaccggt aaccggtact
     2101 caccgcgcac acccgtcccg cccggcgctc cccaccgggc gggacgggtg tgttcgggcg
     2161 gtcaggttcc ggcgaaagcg gtgaacagcc gggccagccg atccggccgg ggaacagcgc
     2221 ctccagtatc gcgaactgct cggccaccac gagcggttgg cgttgggagc gagagctcga
     2281 tacgcggaaa cggctgaacg tcggccgtcc ggccggaatt cggctgcggc ggtggcctcg
     2341 gatcagctgg tgcgtcgctt ggtgtggctt cgagcgccac ggacctggcg gttcaggcca
     2401 ccgcggggcc cgacgtggct cgggtcggtc tgcttggcct tcgccctgcg ggcggcgcgg
     2461 ttgagcggct cgggctcact gaccggctcc gcatcggcga gctcacgctc ggcgccaggc
     2521 tccggctgct ccggacggga cggcttcggc tgagacatgg gacggacctc cgagttcgtg
     2581 gggtggcggt gcgggtggcg ctggctcaga ggatcaccag ccgagggtac cgggagggcg
     2641 gatcgcggtc gcaccagttc cgtcggggtg acggcccacg ctcctgccat ggacgtcggg
     2701 tatcacgtcg acgagctcga acggcagggc gtggcgctcg ccgcggcggc ggaacgcgcc
     2761 gggcccgagg acggtcctgc cgactgcacg gtccgtgggc cggcgagcga gctatacctc
     2821 acgctgtgga accggccggg ggccccggtc gtgcagggcg aaccgaggta ctcgcgctgt
     2881 ggcgcgagcg ggcgcacacc acctggagct gatcggcctc gggtagcgca cggccggtca
     2941 gcccgcgagg ccgcggcggg ccagcaacgg cgcgatctcg gcgtcgcggc cgcgaaacgc
     3001 gcggtaagcc tccatcgggt cgacggatcc gccgcgcgag agcagctccc ggcggaacgt
     3061 ctcgccgttc tcccggcgca gcccgccgtt ctcggcgaac cactccacgg tgtcggcgtc
     3121 cagcacctcg ctccagatgt aggagtagta gcccgcgctg tagccgccgc cgaagatgtg
     3181 gttgaagtat gtgctgcggt aacgggccgg agcggtcggc accgccaccc cagccgcagc
     3241 gatcgcctcg ccctcgaact gctccacgtc ggtgacctcg tcgcctcgac cgagccggtg
     3301 ccacgcctgg tcgagcagcg ccgccgccag gtactcggtt gtggcgaagc cctctccgaa
     3361 ctgccgcgaa tcggccagcc gcgccaccag ctcgccgggc agcggctcac ccgtctcgtg
     3421 gtggcgggcg tagttcgcga ggacctccgg gtcctccagc cacatctcgt tgacctgcga
     3481 cggatactcg acgaagtcgc ggggcaccgc cgtgccggcg aacgtcgggt actgcacatc
     3541 ggagaacagc ccgtgcagcg cgtgcccgaa ctcgtggaag agcgtccgga cgttgtcgat
     3601 ggtgagcagc gtcggttgcc cgtcggcggg gcgcgcgagg ttgagggtgt tgagcacgac
     3661 gggcttcgtg cccacgagcc gcgactgcgt gacgaaagag ttcatccacg ccccaccacg
     3721 cttcgactcg cgggcgtaga ggtcggcgac gaacaacccg agctgcccgt tctcgtcgaa
     3781 gacgtcgaag acgcgcacct ccgggtggta acgcggcagg tcgtggcgct cgctgaagcg
     3841 cagcccgtag agccgtcctg cggcgtggaa gacgccgtcg tgcagcaccc gctccagctc
     3901 gaggtagggc cgcagcatgt cggcgtcgag gtcgaagcgc tcgcggcgga cgcgctcggc
     3961 gtggaacgcc cggtcccatg cctcgatcgg gtgacccgcc gcggccgaca gctcgtcggc
     4021 ctcggcctgc gcgttcgccg ccgccaccgg ggccatcttc ccgagcatgg cgtcgatcgc
     4081 gtcgaccgtg cccgctgtgc cgatctcgac cacccaggac gcatggtgcg ggtggccgag
     4141 cagcgcggcg cgctcggccc gcagcgcggc catgcggagc acgatctcgc gggtgtcgtg
     4201 ctcgccgccg agcccgcggg tgaccgacgc gcggtgcacc cgctcgcgca gcgcccggtc
     4261 ggtgatcgag gcgagcaccg gctgcccggt cggcagcacg aggctgatca cgtgcgcacc
     4321 gtcgtgcccg cgggcggttg cggcacgtgc ggcggcggag acggcatccg gggagagccc
     4381 ggcaagctcc gcgggatcct cgacgaggac cgccgcgtcg ttcgccccgg ccagcagctt
     4441 cgcgccgaac tgcgtggaga gtgtcgacag ctcggcgttg agcgcccgca accgctcctg
     4501 ctcggcggcg cccagccggg ccccggcccg cacggcgtcg cggtggcgac gttccagcaa
     4561 ccggagcgac tccgcgtcca ggccgaggtc gttgcgtgtc gcgaagaggg tctcgatgcg
     4621 ggcgaacagg gccgagtcga gcatgatcgc gtcggagtgc gcggcgagct gcggcgcgat
     4681 ctcggcctcg atcgcccgga cccccggggt gctgcaggag ccgacgagcg tgaagaagac
     4741 cgccgagacc cggcgcagca ccgcgcccga gcgctcaagc gcgacgatcg tgttgtcgaa
     4801 ggtcgcggcc gcggagtcgg cggtgatcga gtccacctcc gcgcgctgct gctccatccc
     4861 cgtcgtgaac gcgggcgcga agtgctcctc acggatggcg tcgaagtccg ggaactcgaa
     4921 gggcagcggg ctgggggcga ggaacgggtt cgcgctcacc agcgcgagca taggtgcgtg
     4981 acgcaggtac gggtcggggt cggagcagtg gtggtcgacg acgggcggat actcgtgctg
     5041 cgccgggccg gggcgcacgg cgcaggcacg tggggactac ccggcggcca ccaggagttc
     5101 ggggagtccc cggaacggac ggccgtgcgc gaggtggccg aggagaccgg cctcgtcgtc
     5161 gaggcgacgg cccggctggg cttcacggac gatccgatgc cggagatcgg gcgccactac
     5221 gtcaccctgt tcatcgcgtg taccagcacg ggcggtgagc tccggctcat ggagtcggac
     5281 aaggccaccg agctcgcctg gctcacccct gacgacctgc tggcgcgccg cgacgagctg
     5341 ttcgtgccgc tgcggcactg tctcgacgcc ggcctgctgg acgtgaagcg atagcgctgg
     5401 acggatcgtg gtcccgtgcc ctgccctgcc cccgcacggc gccgctgcgg cgtcctgacg
     5461 agcaccggca cctcccggcc cgcaggtccc ggggaggagc agcccccgtc agctccgcag
     5521 cagtgtcgcc gctttctccc cgatcgcgac ggcggtggcg gctgtgccgc gccgcggcac
     5581 gaccggcagg accgacgtgt cggccacccg cagtcctctg acgccgtgca cgcgcagctc
     5641 ggcgtccacc accgcaccca tcgcggcgct cccgctgagg tgctgggcgg tgctcaggtg
     5701 ggcggcgatc cagccgtcga gcgcgcggtc ggagcccagg acctcgccgg tgggggcggt
     5761 gcgggtgccg agcccggccc gcagcagctc ggccgcggtg cggatcgcat gacgcagccg
     5821 gcggcggtcg tgttcggtgc gcagataagc gtaggaaatc cgcgggcgga tgcgcgggtc
     5881 gggcgaggtg agcgtgatcg tgccgcggct gtcgggctgc tgcagcatgc acatcaggtg
     5941 caggtcggca tcggtgatga acggccgcac gaacaccagc acctcgacgt caccggccgg
     6001 gtcggcaccg gcgtccaggt tgagcgccgc ctgacagccg ggggcgtgcg ggtgcgccgg
     6061 tgggtctgcc gtgctgaacg ggaggaacac ggccgggtgg tccgaccagc cccggcccac
     6121 cccgggcagc ccacacacga cgggcaggcc cgcggcgcgc agatcgtcgg gcggcccgat
     6181 cccggacagc agcagcaggt gcggcgtggc gatggcacca gcggcgagca caacctcccc
     6241 cgcctcgagc accgtgccat cgagcagctc gacgccgcgt gtccggtcgc cgtcgaaccg
     6301 cacccgggcc acgccggcgc ggggccgcac caccaggttg gctcggtggc ggtccaggta
     6361 agcgtgcgcg gcgctcacgc gcacaccgtc gacgctgttg ctcgggacga gccccgcacc
     6421 gggtgggacg cccgcattct tgtcaggttc cgcaggaaag ccgatgcgtt cggcggcgtc
     6481 gaggaaccgg gtcgcggccg ggtgcagcag accacccgcc ggacgccgga cgggcaccgg
     6541 gccgcggtcg ccgtgccagg gcgtcgcagg gtggtcgagg tcgtgctcgg agcggatgta
     6601 gtgcggcagc agatcggccc acgtccagcc ggggacggtc cagccgtcgg catcggcggg
     6661 cgtggcccgc gcccagatgg caccgttgat cgcgctcgac ccaccgagcc ctcgcccgcg
     6721 cggcaccgtg gcggtgcggc ccggccgcag ctgcgcggcg tatgcccagt tgctcgggtg
     6781 cccggcccgg gtggcagcca gcgatgccgc gtcgagcacg tccggcatgg tctccgccgg
     6841 gccggcctcc aggagcagca cctgcttcgc cgggtcctcc gcgaggcggc ccgcgagcgc
     6901 gcaaccggcc gaacccgccc cgatgacgac cacgtcgaac tcctgccgag gcacgtggga
     6961 gaggatgcac ccgatgagca atctcgcgca ggacctcgcc gacttcatct ccgcggcgcc
     7021 caccccatac cacgccgtgg ccgagggtgt ccgccgcctg accgccgccg ggttcgccga
     7081 gcagcccgag gccggcccgt gggacgacgg tcccggcggc cggtacctcg tgcgtgacgg
     7141 cacgctgctg gcctggttcg tcccggagga ggcgccgccc ggcaccccgg tgcgggtctt
     7201 cgcggcacac accgactcac cgacgctcaa ggtcaagcct cgcccggacg tcggggcggg
     7261 cgggtggcgg cagatcgccg tggaggtcta cggcggcgcc ctgtggaact cctggctcga
     7321 ccgtgacctg gggctcgccg ggcgactcgc gctctacgac ggcacgacgg tgctcgtcga
     7381 cgtacaccgg ccgctgctgc gggtgccgca gctcgccatc cacctcgacc gccaggtgaa
     7441 ccagggtctg aagctggatg cccaggagca cctgctcccg gtctggggcc tgggctcccc
     7501 gagcgacggc gacctgatcg agttcctggc ggacgaggtg ggggtcgagc ccgacgaggt
     7561 ggccgcgcac gacctcgtgg tgcacgacgt caccccgccc gccctcctcg gcgaggagga
     7621 ggagctcctg gccgcgcccc ggctggacaa cctcgcctcc gtgcacgcgg gcctcggagc
     7681 cctcctcgcg gcggcggacg acgtctcggt ttccgggcag gccgtgatcc cggtgttcgt
     7741 cggcttcgat cacgaggaga tcggcagcgg ctcggccagc ggcgcggcgg gaccgctcct
     7801 ggagaccgtg ctcacccggc tggccggagg tttcgacgcc cgcggcgcgg cgttcgcggg
     7861 ctcgcggtgc ctgtccgtgg acgtcacgca tgcggcgcac ccgaactacc tcggccacta
     7921 cgaccccacg caccgggcgc tcccgaaccg cggcccggcg ctgaaggtca acgccaacca
     7981 gcgctacgcc accgacgccc ccggggcggc cgcgtggcac cgcgcctgcc gcgacgccga
     8041 tgtaccgacg caggtgttcg tcgggaggaa cacgttgccg tgcggcagca ccgtggggcc
     8101 gctgctcgcc acgcggctcg gcatccggac cgtcgacgtc gggatcccgg tgctgtcgat
     8161 gcactccgcc cgcgagctgt gcggggtgcg cgaccccgac cacctggccg cggcggccac
     8221 ggcgttcctc accgacccga tgtgaccgtt cccgctccgg acagcagcgc gccgagctgg
     8281 gacgcgaact cctctgggtg catcgcgtat ccgacgtgat cgcccgcgag gtcgacgacc
     8341 tccagcccca gccgttcggc gagcacgagg ttggggcggt agggcatgtg cacgtcggcg
     8401 aggcgcctgt cgtcgtccac gggaccgacc agcggagtgc ggacgaaccc gcggcggtcg
     8461 taggtcagcc gggcgccctg cacgtccagg gactcgatga tgggctcgtc cacgcgccga
     8521 ctgtgccacg cgcctacgcg ggccggaact gctgaccggc gcccgtccac cgatctccga
     8581 cgtccgattg ccggttgagc tcggcataga cccggccacg cacgtaccgt gttgcgggtg
     8641 gaaccggagc aacccacgac accgggcggg ctcgacgacg agacgctggc gttcgcgcat
     8701 cggatgttcg acctcgcgcg cgacggccac accgacgtgc tggccgccca catcgatgcc
     8761 ggcgtcccgg cgaacctcac caacgacaag ggcgacacgc tcctgatcct cgcggcctac
     8821 cacgggcatg cggagacggt ggccgcactg ctggcgcgcg gcgccgatca cgcccgcgtc
     8881 aacgaccgtg ggcagacggc cctggctgcc gccacgttcc accggtcggc cgatgccgtc
     8941 accgccctgc tcgatgccgg cgccgatccc gagcagggca gccagtcggc cctcgccacc
     9001 gccatgttct tcgagctccc cgagatgctg gagctcctgc gtcgggagga aggcccgtcg
     9061 tgaccgcgga ggaggcctcc ctcacgcgcg ggcgcgacgt ctactggttc gtcggcggac
     9121 cgctcgacgg ccgagtggac acccgcgcgc cgtccgagcc ggcgccgtgc accgttcggt
     9181 acgtccatct gcacgacgga ccgaagatcg tgcaccacta cgacctgcac caggtggcgg
     9241 gccacggcgg cgagtaccgg tttcgctact cctcggcgga cgagagcgac gaccctatga
     9301 gtgacgagct catcagcggc gacgcggagg acgacaccag ctgagggcta ccgctgctgt
     9361 ggatcaccgg cccggtcccg acggaacgcg gcagcggctg tcgggtcgcg cttcgccgcc
     9421 gatggtgctg tgctggtgat gcgccgcggc cgacgcttcc gccgcggacc caccgggcga
     9481 ggggacgcga tgacgggcga actgcaggac gacgggctgt tcgggcccga ctccgtgacg
     9541 tggcgcgtgc acctcgagcc ggtgctatgg gtcggcgggt tccgggcact gctgttgcag
     9601 tcgctgcacc cgcgcgtgat gcgcgggacc taccagaact cggcgctgtt cgacccgcgt
     9661 aaggcgtggt cgaggttcca gcggaccgtg gagttcgtcg gcgtccggac attcgggtcc
     9721 acgcccgccg tcgaggccgc cgccgcccg
//
LOCUS       DASRSC010000067         4763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_840274,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000067 DASRSC010000000
VERSION     DASRSC010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4763
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840274"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFE39_03310"
     CDS             <1..374
                     /locus_tag="VFE39_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6257659.1"
                     /translation="NKNLIDVRSPDEFSGKILAPAHLPQEQSQRPGHVPSAINIPWSK
                     AANEDGTFKSDEDLTVLYGGAGYEEGRATIAYCRIGERSSHTWVVLHELLGYSDVKNY
                     DGSWTEYGSLIGVPIELGAGR"
     gene            374..673
                     /locus_tag="VFE39_03315"
     CDS             374..673
                     /locus_tag="VFE39_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1416 domain-containing protein"
                     /protein_id="HET6257660.1"
                     /translation="MCGAPDQAVALPPGTDLTKETVLTGRVVAGGEAVPGAFVRLLDG
                     TGEFTAEVVASAEGNFRFFAAPGRWTVRALSRAGNGETVLDADGPGVHRAEVAVA"
     gene            788..919
                     /locus_tag="VFE39_03320"
     CDS             788..919
                     /locus_tag="VFE39_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257661.1"
                     /translation="MNWFFSGLLIAASAGVVVFTGYLLRRLFTTEPGTPDVPMEAAP"
     gene            937..1542
                     /locus_tag="VFE39_03325"
     CDS             937..1542
                     /locus_tag="VFE39_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FABP family protein"
                     /protein_id="HET6257662.1"
                     /translation="MAGTVPGPANAAPDSPRRNRPRFDDLPVPDDTANLREGPDLHER
                     CLGLLPLIGVWRGEGEVVYPTIDGPYRYGQQIVFAHDGRPFLSYESRAWLLGPDGEVL
                     RPAARETGFWRPQPDGELEVLITHATGITEVFYGRAGSLTSWEIETDAVVRTASAKEV
                     TGSHRLYGLVEGGDLAYVDERAMEGQPLQPHLSARLSRIAG"
     gene            complement(1557..2582)
                     /locus_tag="VFE39_03330"
     CDS             complement(1557..2582)
                     /locus_tag="VFE39_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257663.1"
                     /translation="MQLPLTTDALVPVGAGVVVAIALVILILFAYRRNRAFHSRDLSG
                     PSAAHWTRDSSNRAVQPAPATVLPPAAHAPAATAGGGPESRSSPDQAGTVSEQRMEAK
                     RAGHQEGDVVTAGEPPPWAAGGTRAAGRSNGADDPAVRAVSEARALPPEVVGSSRTVA
                     AAVAQAFAVRAANARRGGEQKPSADAGRNVVPPGADDHNGTPPVGADRNGRLSVGADR
                     NGALSGSADRNGALSGSADRNGALSGSADRNGGLSGDADAKATGSSTLSPPSTTRGDA
                     RDRLLAVLLDDPVRAVGATEELEACRSRLRDVMHRLAGTGLRPEQLARLAGLPVEDVH
                     KMLEPAA"
     gene            4416..>4763
                     /locus_tag="VFE39_03335"
     CDS             4416..>4763
                     /locus_tag="VFE39_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HET6257664.1"
                     /translation="MLDTARESSTLRRTLHQRGLRMTPQRQLVLDAVRALGHATPEQI
                     CAQVQRAAPAVNITTVYRTLDLMERIGLVRHTHLGHGAPTYSEQEHRHVHLVCHSCGA
                     VTETPTDLMDDLAE"
BASE COUNT          649 a         1688 c         1753 g          673 t
ORIGIN      
        1 acaacaagaa cctgatcgac gtgcgctcgc cagacgagtt ctcgggcaag atcctggcgc
       61 cggcgcacct gccgcaggag cagtcgcagc ggcccggcca cgtcccctcc gcaatcaaca
      121 tcccgtggag caaggcggcg aacgaggacg ggaccttcaa gtccgacgag gacctcacgg
      181 tgctctacgg cggggccggc tacgaggagg gcagggcgac gatcgcctac tgccgcatcg
      241 gcgagcgcag ctcccacacc tgggtggtgc tgcacgagct gctcggctac agcgacgtca
      301 agaactacga cggcagttgg accgagtacg gctcgctgat cggtgtgccg atcgagctgg
      361 gagctggtcg ctgatgtgtg gagcacctga ccaggccgtc gccctccccc cgggcacaga
      421 tctgaccaag gaaaccgtcc tgaccggtcg agtcgtcgcg ggaggcgagg ccgtccccgg
      481 tgcgttcgtc cggctgctcg acggcacggg tgagttcacc gccgaggtcg tcgcgtcggc
      541 cgagggcaac ttccggttct tcgccgcgcc cgggaggtgg accgtgcgcg cgctgtcccg
      601 tgcaggcaac ggggagacgg tcctcgacgc cgacggcccc ggcgtgcacc gggccgaggt
      661 tgcggtggcc tgagcgtccc cggagttgca gggagagcca cgttccggcc gtccggctga
      721 gtgaacatgg ctctccctgc gcgtggggtc ggcgtcgtcc gcccgacacg cactaggctc
      781 cgcgcacgtg aactggttct tctccggcct gctgatcgca gccagtgcgg gtgtcgtggt
      841 cttcaccggc tacctgctcc ggaggctctt cacgacggaa ccgggcaccc ctgacgtacc
      901 catggaggct gccccgtgac cggaccggag ggagacctgg cgggcacggt ccccgggccc
      961 gccaacgcgg caccggactc accgcggcgc aaccggccgc gcttcgacga cctgccggtt
     1021 cccgacgaca cggccaacct gcgggagggc cccgacctgc acgaacggtg ccttggcctg
     1081 ctcccgctga tcggcgtgtg gcgcggcgag ggcgaggtcg tctacccgac gatcgacggg
     1141 ccgtaccgct acggccaaca gatcgtgttc gcccatgacg ggcgcccgtt cctgtcctac
     1201 gagtccaggg cgtggctgct cggtccggac ggcgaggtgc tgcgccctgc ggcgcgggag
     1261 accggtttct ggcgtccaca gccggacggc gagctcgagg tgctcatcac gcacgccacg
     1321 gggatcaccg aggtcttcta cggccgtgcg gggagcctga cgtcctggga gatcgagacc
     1381 gatgcggtag tgcgcacggc gtcggcgaag gaggtgacgg gctcccaccg gctgtacggg
     1441 ctggtcgagg gtggcgacct cgcctacgtc gacgagcggg cgatggaggg ccagccgctg
     1501 cagccacacc tctcggcacg gttgagccgt atcgcgggct gacgctacgg cacgagctaa
     1561 gcggcgggct ccagcatctt gtgcacatcc tcgaccggga gcccggcgag gcgcgcgagc
     1621 tgctccgggc gcagaccggt gccggccagc cggtgcatca cgtcgcggag ccggctgcgg
     1681 caggcctcca actcctcggt ggccccgacc gcgcgcactg gatcgtcgag gagcacggcg
     1741 aggagccggt cccgggcgtc gccgcgggtg gtactgggcg gcgacagcgt cgatgacccc
     1801 gtggccttgg cgtcggcatc gcccgacagc ccgccgttgc gatcggcact gcccgacagc
     1861 gcgccgttgc gatcggcact gcccgacagt gcgccgttgc gatcggcact gcccgacagc
     1921 gcgccgttgc gatcggcacc gaccgacagc cggccgttgc gatcggcacc gaccggcggc
     1981 gtgccgttgt ggtcgtcagc gccgggcggc acgacattgc gaccggcatc tgccgacggc
     2041 ttctgctcac cgcctcgacg cgcgttcgcg gcccgcaccg cgaacgcctg cgcgaccgct
     2101 gctgccacgg tccggctaga tcccactacc tccggcggga gggctcgcgc ctcggagacg
     2161 gcccggacag ccggatcgtc cgcgccgttc gaccttccgg cggcacgggt gccacccgcc
     2221 gcccatggcg gtggctcgcc cgcggtcacg acgtcgccct cctggtggcc ggcccgcttc
     2281 gcctccatcc gctgctccga aacagtcccg gcctgatcgg gcgacgaccg cgactcagga
     2341 ccccctcccg cggtggcggc cggggcgtgc gcggccgggg gcagcaccgt cgccggggcg
     2401 ggctggacag cccggttcga ggaatctctc gtccagtggg ccgcgctggg cccggacagg
     2461 tcgcggctgt gaaaagcgcg gttgcgccga tacgcgaaca agatcaggat gacgagcgcg
     2521 atggccacca ccacgcccgc acccaccggg accagcgcat ccgtcgtcag gggcagttgc
     2581 atgggagcgg accgtacgcg ttctcaactc acggtgcgca atcgcaatca gccagagcgt
     2641 gacgcagatg tcggacgatt tgaccagtcc gaaccgctcg gtccggggcc ggcgcccgct
     2701 ccgccgtcgc cacgaacgcc gtcatctgtg ccgccggacg tggggttctt ctggttggtg
     2761 cctcctgagg cgggtgtgac gggtggggtc aggctcgtcg cctccgagcc gctcgacgag
     2821 cggaccgaat tcaccttgac ggtggtgtct acggccccgg ctttcccggc aggccggtcg
     2881 gtttcggcga ctggggtgga cctgtcggcg ggccgcacaa ctggaaccaa cgtggtgcgg
     2941 tcgccgctcg gcacaccacc ggcgcggggc gccccggtgc tctgcggctc gtctgcttct
     3001 cccgcgacag gacggaacag atcggcgggc tccgggtcgg actcggcgcg tggacgcatc
     3061 gagccggact gctcgccgac ggcctggtag ccgccggagg ggaggcggtg ggccgcggac
     3121 ttgctggcgg gggccgagct ctggtcgcca ccgggggacg gcttcggcgg ggaggaggtg
     3181 cccgatgatg gacgcccgga cgcggacgat gatgcgtgcg agtcgccggg gtggtgcgcc
     3241 gtcgaagccg actgggccgg cggggcggct gtcggcttcg gggccggtac gtcgggccgg
     3301 gccgaccggt tcggcgccgg cgcgctcgct ccgggaccgc tgctcgccga cgtcgaaccg
     3361 ctccgggggg atccgccctg cccgcctccc ccgtgcggcc cagctgtacc gggaccaggg
     3421 tgcgaggagc cgccctggcc ggggcgcgat gagtcggcct gcgatccgtc cggcgtcgat
     3481 cccgtgctgc cgggccgggg gccctgcgcg gagctctctg cggcacgggt ggacggccct
     3541 ggcgatccgg cgccggaggc ccgagtaccg gaggacgacg gcgagccgcc cgatggcgcc
     3601 ggggcgggct gcggcacgcc gtgctggccg ggggcgcccg gcagggacgg cgccggcgcc
     3661 ggggtgtccc gcggccgcgt cggggccggg ctgctgtcac gcggcgggag ggtgatgcga
     3721 gccgtcggcg cagcgagcat gccctcgagc tgaccgcgca tacgtgtgag ctctgcgagc
     3781 accgcgtcgc gctggcggac caggtcggcg agctgcgccc ggacgttctc ctcctcggcg
     3841 cgcgcagcgg agcgggcctt cttgcggatc tccgcggcct cgtcctccgc ggcctgcagg
     3901 atgctctgca tccggtccgt gaagttgttg atcatgtcgg gtgactggcc gggaggcatc
     3961 ggacccccgg gtcgcggacg cggggtgggt cgcggacgag caccgggcgg aacctgctga
     4021 cccggtgccg gaagaccgac cgacgggggc tggacgagcg ggatcggctg gctgcgggcc
     4081 atctccaggt cggctcgcat ccgggagacg acacgctgca ggcgggcaac gtgctcgtcg
     4141 acctgccgac ggtcgtaacc gcggaggacg acgtcgaacc gtggggcgcc aggcactccc
     4201 gcgtcagtcg tcatcgtcgg cccctcccga gcgtactgtc accctgcatt tcgccgaggg
     4261 taggtagatc gtcgcgctca aacctgttgg ttcaccctga cggagcatac cgatagtgga
     4321 cggtttcact cgtcgccgga tcgcgggcac ccgtccgcag cattcggaca gccgggaccg
     4381 gacggcctac catcgaaccg gtggagactc cgtcgatgct cgacaccgcg cgtgaatcct
     4441 cgacgctacg acgaacgttg caccagcgcg gcctgcgcat gactccgcag cgacagctgg
     4501 tcctcgacgc cgtccgcgcg ctcggtcatg cgacgccgga gcagatctgc gcgcaggtgc
     4561 agcgggccgc gcccgctgtc aacatcacca cggtctaccg caccctcgat ctcatggaac
     4621 gcatcgggct cgtgcggcac acacacctcg gccatggcgc gcccacctac tccgagcagg
     4681 aacaccggca cgtccacctg gtctgccact cctgcggcgc ggtcacggag acgcccaccg
     4741 acctgatgga cgacctggcc gag
//
LOCUS       DASRSC010000068         5322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1496961,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000068 DASRSC010000000
VERSION     DASRSC010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496961"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="VFE39_03340"
     CDS             <1..151
                     /locus_tag="VFE39_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HET6257665.1"
                     /translation="PDGGGADVFVHYSAIQTNGYKSLDEGQRVSFDIEQGQKGPQATK
                     VTPLG"
     gene            complement(453..1163)
                     /locus_tag="VFE39_03345"
     CDS             complement(453..1163)
                     /locus_tag="VFE39_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6257666.1"
                     /translation="MPVGSGTPRQRYRDQVRAEIKEAALAQMYIGGAEALSLNAVAKE
                     LGVTGPAIYKYFRSRDDLLTELVFDSYGEAAAAVRAEAERTPHRPPRERLHALAEAYR
                     QWAIAAPHLFQLLAIMPSPTVDSPPETIERAKGVLGPFLPVLGHGRTWPAAAELREQV
                     QGWIDRRDAVADWVRTYAPEADPATALTGALIVWVRLHGVVSLEIQGKFTGMGHQPAT
                     LLTVEMESIADSMALPTT"
     gene            1527..2798
                     /locus_tag="VFE39_03350"
     CDS             1527..2798
                     /locus_tag="VFE39_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6257667.1"
                     /translation="MLPDVTCGGASTAAVRSGPAAVSPATLRRLELAAGDLALACLLA
                     MEEHQPWFARLPAEQRSAVLLVTQTGVANFVAWLGERGESIRLTAEAFRIAPRDLARR
                     LSLRQTVDLVRIATDVFERHLPPLAADEAERHALVEGVLRFGREIAFAAATVYAGAAE
                     TRGAWDARLEALVVDAVVRGPEIEGGVDDALVSRASTLGWDPDAAVRVVAGSPAPDSA
                     AERLGELRREAGRAGRSVLVGVQGRRLILLLSEPHGQPGGGDIGEVIGDIGGLVDGFG
                     PGPVVVGPVATELATAHNSARDALSALRAAPGWPDAPRPVAADDLLPERALGGDVSAH
                     RTLVESIVAPLETAGGELLHTLATYLEGGGALEACARALFVHPNTVRYRLRRVCDLTG
                     RSPSDPRDALVLRAAVIAARLNPVGEPGPPT"
     gene            2950..3882
                     /locus_tag="VFE39_03355"
     CDS             2950..3882
                     /locus_tag="VFE39_03355"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET6257668.1"
                     /translation="MIALLAPGQGSQNPGMLLPWLDDPTAEETLARWSGAIGLDLRHL
                     GTEADAEEIKDTAVTQPLVVAAALLAAARLFARVGLPVAAPVAGHSVGELAAAAVAGV
                     LTPDAAVALAAVRGREMATACALEPTGMSAVLGGKPDEVLARLDELGLDPANRNAAGQ
                     VVAAGSVAALAALAEDPPERARVKPLPVAGAFHTRFMAPAEEAMRSHAAGVSPADPTR
                     PLLSNSDGTVVTDGAEALRRLVAQVTRPVRWDSCMATLRELGVTAVIELPPAGALVGL
                     VKRDLKGTATLALKTPDDLAAAVDLITEHAGADK"
     gene            3879..4865
                     /locus_tag="VFE39_03360"
     CDS             3879..4865
                     /locus_tag="VFE39_03360"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005451665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HET6257669.1"
                     /translation="MTQLRLAPTAPGARILGLGSSQPDNVVTNDDLAQRIDTNDEWIR
                     ERVGIESRRIAEQGTLLVDMATEAGERAVKDAGLAPNEIDTVIVATCTMPTPIPNAAA
                     QTADRIGIPAPGAFDLNAACAGFCFGLGAAADLVRGGSAKNVLVIGAEKLSDWLDWTD
                     RSTAIIFADGAGAAVIGPAPDEASVGVGPLAWGSAGDMAQAIHVKPENNALFQEGQAV
                     FRWATTKIAPVALRAIELAGLTPADIDVFVPHQANLRIVDAVAKRLRAKGARDDMVVA
                     DDIRHSGNTSSASIPLALDHMRADGRVRSGDTVLLVGFGAGLSYAGQVVVCP"
     gene            4911..5153
                     /locus_tag="VFE39_03365"
     CDS             4911..5153
                     /locus_tag="VFE39_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET6257670.1"
                     /translation="MADNTEILSGFAEIVEEVAGIDTAEVTIEKSFVDDLDIDSLSMV
                     EIAVQAEDKFGVKIPDDQLAELKTVGDAVDYIAKNQ"
     gene            5187..>5322
                     /locus_tag="VFE39_03370"
     CDS             5187..>5322
                     /locus_tag="VFE39_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HET6257671.1"
                     /translation="MSTTPDVVVTGLGATTPLGGDVASTCEALLAGRSGVRRIDDADH
                     E"
BASE COUNT          756 a         1963 c         1855 g          748 t
ORIGIN      
        1 tcctgacggt ggtggcgcgg acgtgttcgt gcactactcg gcgatccaga ccaatggtta
       61 caagagcctc gacgagggcc agcgcgtgtc gttcgacatc gagcagggcc agaagggtcc
      121 gcaggccacc aaggtcactc cactcggctg aggccgagct gccgggagcc cgggtagctc
      181 ccgacattcc cacacgcccc ggaccgtcgt agcgtcgtcg ggccgaggcc ctcacggacg
      241 tccgttggtc gcaatcgacc accggcacat ctgctcgccc tgatcgggtc cgcggcctcc
      301 gccttgctcc cggtcggcgt ccatgcgccg gtggtgggcc cgcggcctcc gccgggcctc
      361 gatccgctgt acccgacagt agcgacgtgc gcacgccgcc cccacacggg ggtggctatc
      421 ccgccccggc gggaacgacc ggctacgggg cgtcaggtcg tggggagggc catggagtcg
      481 gctatcgact ccatctccac ggtcagcagc gtggccggct ggtgacccat gccggtgaac
      541 tttccctgga tctccaggct gacgacaccg tgcaggcgca cccagacgat cagcgcgccg
      601 gtgagtgcgg tggcggggtc ggcttcgggt gcgtaggtcc gcacccagtc ggccacggca
      661 tcgcgccggt cgatccaccc ctgcacctgc tccctgagct cggcggcggc gggccaggtg
      721 cgtccgtggc cgaggacggg gaggaatggg ccgagcaccc ctttggcgcg ttcgatcgtc
      781 tcggggggcg agtccaccgt gggggagggc atgatcgcca gcagctggaa gagatgcggc
      841 gcggcgatgg cccattgccg gtacgcctcg gccagtgcgt ggagtcgctc gcgcggcggc
      901 cggtgcggag tgcgctcggc ctcggcgcgc acggcggcgg cggcctcgcc gtaggagtcg
      961 aagacgagct cggtcaacag gtcgtcgcgg ctacggaagt acttgtagat ggcaggcccg
     1021 gtgacgccca gctccttcgc gacggcgttg aggctgagcg cttcggcccc gccgatgtac
     1081 atctgggcca gcgcggcctc cttgatctcg gcacgcacct ggtcccgata gcgctggcgc
     1141 ggcgtgccgc tgccgaccgg catccgaccc accctcctcc ggcccatctc gacgtcggtg
     1201 gcagagcttc agaacatgat ccatgtccgt cagcccgcga cagggacctg ccgttgccga
     1261 cagaccgccc gcctaccgtg cgcggcgccg ggcagtagtc gcacgcgcgg cagacaggac
     1321 cgcactcatc acccagatgg acgtcacgac cacgtccgcc ccgggcacga tccgctgccc
     1381 gttcaccaac agtaatacgt tctgcctccc gcaacgctag tgctcccgct catttcgagc
     1441 cccatccgcc gccggccatg ggcaggtaca cggcccggcc gccagagcta cgactctcag
     1501 cgccccggcg ttcctccagc cgtgcgatgc tgcccgacgt gacgtgcggt ggagccagca
     1561 cggcggcggt gcggtccggc ccggccgccg tctccccggc aacgctgcgc aggctcgagc
     1621 tggcagcagg cgacctcgcg ctcgcctgcc tactggcgat ggaggagcac cagccatggt
     1681 tcgcccggct accggccgag cagcggtcag cagtgctgct ggtcacccag accggcgtcg
     1741 ccaacttcgt cgcctggctc ggcgagcggg gcgagtcgat ccgcctcacc gcggaggcct
     1801 tccgcatcgc gccgcgtgac ctcgcgcgga gactgagcct gcgccagacc gtcgacctcg
     1861 tccgcatcgc gaccgacgtg ttcgaacggc acctcccccc gctcgccgcc gacgaggccg
     1921 agcgccatgc gctcgtggag ggcgtcctgc gcttcggccg cgagatcgcc ttcgccgcgg
     1981 ccacggtgta cgcgggtgcg gccgagaccc gcggcgcgtg ggacgcccgg ctcgaggctc
     2041 tcgtcgtcga cgccgtggtg cgcggtccgg agatcgaggg cggggtcgat gacgccctgg
     2101 tctcccgcgc ctccacgctg ggctgggacc cggacgctgc ggtgcgggtg gtggccggca
     2161 gtccggcgcc cgactcggcc gccgagcggc tcggtgagct gcgccgcgag gcagggcggg
     2221 cgggtcgctc ggtgctcgtc ggcgtgcagg gcaggaggct gatcctgctg ctctccgagc
     2281 cgcacggtca gcccggcggc ggggacatcg gcgaggtcat cggggacatc ggcgggctcg
     2341 tcgacggctt cggtccaggt ccggtggtgg tcggccccgt ggccaccgag ctcgccaccg
     2401 cccacaacag cgccagggac gcgttgtccg cgttgcgtgc ggcgccggga tggcccgatg
     2461 cccctcggcc ggtcgccgcc gacgacctgc tgccagagcg ggcactgggc ggggatgtgt
     2521 ccgcgcaccg cacgctcgtc gagtcgatcg tcgccccgct cgagacggcg gggggcgaac
     2581 tgctgcacac actcgccacg tacctggagg gcggcggagc gctcgaggcg tgcgcgcggg
     2641 cgctgttcgt gcatcccaac acggtgcgct accggctccg tcgcgtgtgc gacctcacag
     2701 gccgcagccc ctccgaccca cgggacgcgc tcgtactgcg cgccgccgtc atcgcggcca
     2761 ggctgaaccc ggtcggggaa ccaggaccac cgacgtgagc tgcggaaggc ctacaacgaa
     2821 ccgtgccggc atcgtggcca actccacgcg cggatcacac cgctttggag gaaacctcca
     2881 atacacgggc acggagttcg tgcgtgccgg tatccccccg gggaccctcc tcagtgtttc
     2941 ctggtgcgcg tgatcgcctt gctcgcaccc ggtcaggggt cccagaatcc cggcatgctc
     3001 ctcccgtggc tggatgatcc gacggcggag gagaccctcg cccggtggtc cggcgccatc
     3061 gggctcgacc tgcgccacct cggcacggag gcggacgccg aagagatcaa ggacaccgca
     3121 gtcacccagc cgctggtcgt cgccgcggcg ctgctcgccg cagcccggtt gtttgcacgg
     3181 gtcgggctgc ccgtcgccgc gcccgtcgcc ggtcactcgg tcggcgagct cgccgccgcc
     3241 gctgtcgccg gggttctcac cccggacgcc gccgtcgcac tcgccgccgt ccgtggccgc
     3301 gagatggcca ccgcgtgcgc gctcgagccc acgggcatga gtgcggtgct gggcgggaaa
     3361 cccgatgagg tgctcgccag gctcgacgag ctcggtctgg atccggccaa ccgcaacgct
     3421 gcgggccagg tggtcgcggc cgggtcggtc gcggcgctgg cagccctcgc ggaggacccg
     3481 cccgagcggg cccgggtgaa gcccctgccg gtggccgggg ccttccacac gcggttcatg
     3541 gcacccgccg aggaggccat gcgtagccac gccgccggcg tctcccccgc cgatccgacc
     3601 cggccactcc tctccaactc cgacggcacg gtcgtcaccg acggcgcgga ggcgctgcgc
     3661 aggctggtcg cccaggtgac tcggcccgtc cggtgggact cctgcatggc gacgctgcgc
     3721 gagctcggcg tcaccgccgt gatcgagctg cctccggcgg gcgcgttggt ggggctggtc
     3781 aagcgagatc tcaagggcac ggcgacactg gctctcaaga ccccggacga cctcgccgcg
     3841 gccgtcgacc tgatcaccga gcacgcaggg gcagacaagt gacacagctc agactcgcac
     3901 ccaccgcacc gggtgcccga atcctcggac tcggcagcag ccagcccgac aacgtcgtca
     3961 ccaacgacga cctggcgcag cgaatcgaca ccaacgacga atggatccgg gagcgggtcg
     4021 gcatcgagag ccgccgcatc gccgagcagg ggacgctgct ggtcgacatg gccaccgagg
     4081 ccggggagcg cgcggtcaag gacgccgggc tggcgccgaa cgagatcgac accgtcatcg
     4141 tcgccacctg caccatgccg acgccgatcc cgaacgcggc cgcgcagacg gccgaccgca
     4201 tcgggattcc cgcacccggc gccttcgacc tcaacgccgc ctgcgccggg ttctgcttcg
     4261 ggttgggcgc tgccgccgac ctcgttcgcg gcggcagtgc gaagaacgtg ctcgtgatcg
     4321 gcgcggaaaa gctctcggac tggctggact ggaccgaccg ctccaccgcg atcatcttcg
     4381 ccgacggtgc cggcgctgcc gtgatcggcc ccgcacccga cgaagcctcg gtcggcgtcg
     4441 gcccgctcgc ctggggcagc gcaggggaca tggcacaggc catccacgtc aagcccgaga
     4501 acaacgcgct gttccaggag ggccaggccg tcttccggtg ggccaccacg aagatcgccc
     4561 ccgtcgcgct gcgggcgatc gagctggccg gcctcacccc ggccgacatc gacgtcttcg
     4621 tcccgcacca ggccaacctg cgcatcgtcg acgccgtggc caagcggctg cgcgccaagg
     4681 gcgcccgcga cgacatggtg gtggccgacg acatccgcca ctcgggcaac acctcgtccg
     4741 catcgatccc gcttgcactg gaccacatgc gcgccgacgg gcgggtgcgc tctggcgaca
     4801 cagtgctcct cgtaggcttc ggcgcgggcc tgtcctacgc cggccaggtc gtcgtgtgtc
     4861 cctgatccgt ccctgaccga cttcccgtcc ccaccagaag gagaaccacc gtggctgaca
     4921 acaccgagat cctcagcggc ttcgccgaga tcgtcgagga ggtcgccggc atcgacaccg
     4981 ctgaggtgac catcgagaag tccttcgtcg acgacctgga catcgactcc ctgtcgatgg
     5041 tcgagatcgc cgtgcaggcc gaggacaagt tcggcgtcaa gattccggac gaccagctgg
     5101 ccgagctgaa gacggtcggt gacgcggtgg actacatcgc gaagaaccag tgacggcccg
     5161 gacgcgatcg ttcccgcgga ggatccatga gcaccactcc tgatgtcgtc gtcaccgggc
     5221 tcggtgcgac gaccccgctc ggcggggacg tcgcgtcgac ctgtgaagcg ctgctcgccg
     5281 ggcgttccgg cgtccggcgc atcgacgacg ccgatcacga gt
//
LOCUS       DASRSC010000069        10042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2315575,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000069 DASRSC010000000
VERSION     DASRSC010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10042
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2315575"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /gene="rsgA"
                     /locus_tag="VFE39_03375"
     CDS             <1..836
                     /gene="rsgA"
                     /locus_tag="VFE39_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET6257672.1"
                     /translation="RPPVTAMRARELGRTPIVVGDRVGLVGDLSGAPDTIARIVRVEE
                     RRTLLRRTADDTDPFERVVVANAEQLVIVTSVADPEPRTGFVDRCLVAAYAGGLAPVL
                     CLTKADLGDPEVFAAQYRELDLPVLVTRRDQEPTELADVLAGRVSALVGHSGVGKSTL
                     VNALVPDAARAVGIVSAVGKGRHTTVAALALPLPEDRGGGWVVDTPGVRSFGLAHVTP
                     DDVPAAFDDLASAIEACPRGCGHLGPPADPECALDALVQQGLLRPGRLAALRRVLVAL
                     R"
     gene            859..1356
                     /locus_tag="VFE39_03380"
     CDS             859..1356
                     /locus_tag="VFE39_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HET6257673.1"
                     /translation="MTTGEESSPLRIGTAERTAAMKALDEHLAAGRLGVEEYADRSAV
                     AANAVVASELAELFTDLPEPHPKLPGTASTAPLTAPLPVVAPPGEVATQRGGFLDGWG
                     PRLVSVTPFLAVALFLITRQWYFFLLIPAMGALVYGGGVGRKDDDRDRDERRRERNQR
                     RRDRR"
     gene            complement(1408..2520)
                     /locus_tag="VFE39_03385"
     CDS             complement(1408..2520)
                     /locus_tag="VFE39_03385"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HET6257674.1"
                     /translation="MVRPVRGRGPGGAGLTGVGLTGGAGRVGLRAQRGRAQLLTPGVR
                     DPRDVVGLLVGIQAQDWRSARLALRARTCGLAAADATPDDGSVVVAWLLRGTLHLVRT
                     EDHGWLLGLTARAGVVESRRRLAEEGVTDRQVDTALQVIGAELACGAARSRAELADRL
                     AAAGVPVVGQAVHHCVRLAALHGQAVLVPAGTSGELLVRAVGRHAEPIDRHVAVVELA
                     RRYLLGHGPATAADLAAWSGLPLRDARAGMSGLGTALVERVDGLADVAERDVAALPAL
                     PPRLLPPFDPYLLGWADRSFAVPEHLRSRVQPGGGMLRACATVDGAVVGTWGLRRRGG
                     RAEVALDVADPAHAEALQPEVADVARFAVGAAPPES"
     gene            complement(2475..2981)
                     /locus_tag="VFE39_03390"
     CDS             complement(2475..2981)
                     /locus_tag="VFE39_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257675.1"
                     /translation="MRVYVPATWPMLRTLVKNGSVDPIGGTAFALTPKLRESYTSGDE
                     EELEYAALTEAARASLRLLAVEFGLGEDGTPARRVVIAADLENVTLRPDLDDGAVRIA
                     GGVPFETIAAVHVDNDEAEYAVRQAAGAVDAADMGDLDAEFLVGEAEDHELAWYGPSE
                     VAALVELG"
     gene            complement(2990..4456)
                     /locus_tag="VFE39_03395"
     CDS             complement(2990..4456)
                     /locus_tag="VFE39_03395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET6257676.1"
                     /translation="MPPRLSALDASFLYLEQPTTPMHVGAVSTFRRPRSAGPLGGFNY
                     DRLVELIEQRIALVPRYRQKVRYVPGNLARPVWIDDPDFDVAYHVRRSALPKPGSDEQ
                     LTELVARLMSRPLDHTRPLWEMYLVEGVARGRTAMITKTHQAMVDGISAFDIGQVILD
                     VSPPGEGDLRLLGENGRRARAPVEELWMPRPEPTHAQLVLGAVAEAVARPGEIVDNVR
                     VAAGDALATVGKITGTAGKLFSIARTASRRAPGSPLNVDISTQRRFATARTQLDSYRR
                     VRAAHGCTVNDVVLTVVSGALRNWLLSRGEPVTPSSSVRAMVPLSVRGEADVPSSAAA
                     GSLGNRVSSFLVDLPVGEPSPVVRLHHVTHAMREHTATGQSVGADTLVRIGGFAPPTL
                     HALGARAASGFSKRIFNLVVTNVPGPQFPLYAAGARMLEMFPVVPLAKGQALSIGLTS
                     YDGGVYYGFNGDRDGMSDIDVLAGMVVESLDELMATVG"
     gene            4497..5279
                     /locus_tag="VFE39_03400"
     CDS             4497..5279
                     /locus_tag="VFE39_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET6257677.1"
                     /translation="MSGARGVPEPAGRSPKDTFITVYGRKPVLEALHDGQLRVDKVIM
                     ADGLQGEPVRAITAAARDRGVPVQRASAHRVKVLAGNGRHDQGVLADVVAPRMAPVAE
                     FLAELAPGAPASVLVLDRLTNPSNVGMILRSSTAAGLDGILLPRRGVPAIDPLVVKAS
                     AGVAFRAPVLRSGTVEEGCAELAAAGFTVYGLDAGGASLLEAELPARVALVLGNETDG
                     LSESVRSLVDGLLAIPMHGGVESLGVASAGAVAAFELARRAR"
     gene            5323..6543
                     /locus_tag="VFE39_03405"
     CDS             5323..6543
                     /locus_tag="VFE39_03405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HET6257678.1"
                     /translation="MVQRRLPRPSELRPLLRPAPFVRDAAERRLLRAASIADLRAIAR
                     RRTPRAVFDYTDGAADGELSLNRARDAFSRVEFTPTVLRDVSSVDTGRDILGRRSNLP
                     FAFAPTGFTRMMHHEGERAVASVAQKVGIPYTLSTVGTTTIEEVAAAAPDARKWFQLY
                     LWRDRAPAKDLVQRAADAGYDTIMLTVDTPVGGARLRDVHNGLTVPPALSLKTFVDGA
                     RHPHWWFNLLTTEPLTFASLESSEGTVAELINRVFDPALTMNDVEWLRDTWPGKLVVK
                     GIQSVEDARRVVDAGADAVLLSNHGGRQLDRAPVPLELVEPTVQAVGDRAEVLVDTGI
                     THGADVLAAIALGARAALVGRAYLYGLMAGGQLGVEKAVTILAGEIVRTLQLIGVNSV
                     DELRPSHVRLRPNG"
     gene            6586..6924
                     /locus_tag="VFE39_03410"
     CDS             6586..6924
                     /locus_tag="VFE39_03410"
                     /inference="COORDINATES: protein motif:HMM:NF025379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET6257679.1"
                     /translation="MGFMDKAKELLNRHDDKVDQGLDKAGEAAKGRFAGHEGQLGQGV
                     DHLKRMTGAGDTTERAPGEPPPGTPPQGPAPEDARPDNPTGGYEPAQEVPGGPQPDVP
                     AGPGNEPPQR"
     gene            6974..7153
                     /locus_tag="VFE39_03415"
     CDS             6974..7153
                     /locus_tag="VFE39_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257680.1"
                     /translation="MADDRRDDRSGPGEEGFLESLLRDATITGTEEDPPPPPAEPQVA
                     APETTNDPDDDTDSS"
     gene            complement(7163..7543)
                     /locus_tag="VFE39_03420"
     CDS             complement(7163..7543)
                     /locus_tag="VFE39_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HET6257681.1"
                     /translation="MPLRGLLMDYAGVLTDGPEVLALVRRARAAGVATALVSDGRTVP
                     DECATTFDAVVLGPALGVRKPDPEIFRRVAAMLGLPVEQCVVVDDHAGNVRGARKAGA
                     VVVHHCDVVTTIGEVEILLDLPPP"
     gene            complement(7592..7831)
                     /locus_tag="VFE39_03425"
     CDS             complement(7592..7831)
                     /locus_tag="VFE39_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257682.1"
                     /translation="MKDVTTLGRGGSGDWVFYSSYGPPEATEQGAVVHGELWTRLGAV
                     QNGHVRLVDDEVWFLGLGPSGAMLVLDDLQKMLTG"
     gene            complement(7828..8292)
                     /locus_tag="VFE39_03430"
     CDS             complement(7828..8292)
                     /locus_tag="VFE39_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET6257683.1"
                     /translation="MTDGFFEPDPQLMPLVRLLSRTGQAVVRYTSRTIAAHGLTTTAM
                     GALGVLADTGAVSHRELSGKLGVAPATLTPVVDALEAAGELARERDPADRRVVRLWIT
                     PSGRARLAEVHGDVVTAFRARLPAPAPGDAELIRAYLLAVLAAVGDEAGTER"
     gene            8396..8908
                     /locus_tag="VFE39_03435"
     CDS             8396..8908
                     /locus_tag="VFE39_03435"
                     /inference="COORDINATES: protein motif:HMM:NF039460.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3235 family protein"
                     /protein_id="HET6257684.1"
                     /translation="MTTTLTATPRPTPATSSGAVRTHRAGAPRLRRMAYEPEPGATAA
                     VPILPVPPPPTPSAALDAAAELAEARHSVGRILRLALEVLDGRRPPVQLTGHVAAPVL
                     RYWRAAAQQRRVCSPARFSRMRLCLPRPGIAEVAVACAMDGRVRALAARFERADNTGT
                     GWRCTALRLG"
     gene            complement(8924..>10042)
                     /locus_tag="VFE39_03440"
     CDS             complement(8924..>10042)
                     /locus_tag="VFE39_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HET6257685.1"
                     /translation="GMVTKAIRSAQTQVEQQNFEIRKNVLKYDEVLNKQRTVIYDERR
                     RVLAGEDIKEQIEHMIEDVVTAYIDGATAEGYAEDWDLEKLWTALGTLYTPGIRWESL
                     TDGKDDNGLSDPGDLTKETLAEAVLADARAAYALREAEVDELIAPLGGMRELERQVLL
                     TVIDRKWREHLYEMDYLKDGIGLRAMAQRDPLIEYQREGFDMFSAMLEGIKEETVSYL
                     FNAQVQPAQPAVVQEPAAAAQPAPAASTQAAPVRQAPVRQAAAQPAAVQQQSPAPRRA
                     APQGAPARPSEAEPVTAPNVLPPAFRGSRPTELRYSGPAEDGSAMRARSGNGGARDGS
                     RGEATVSGSGEPSRNRPCPCGSGRKYKQCHGSPTAARP"
BASE COUNT         1351 a         3800 c         3565 g         1326 t
ORIGIN      
        1 cccgaccgcc ggtcaccgcg atgcgcgccc gtgagctggg ccgcacgccg atcgtcgtgg
       61 gcgaccgggt cgggctcgtc ggcgacctgt ccggtgcccc ggacacgatc gcccggatcg
      121 tgcgcgtcga ggagcgccgc accctgctgc gccgcaccgc cgacgacacc gacccgttcg
      181 aacgggtggt ggtcgccaac gcggagcagc tcgtcatcgt cacctcggtg gcggaccccg
      241 agccgcgcac cgggttcgtg gaccgctgcc tggtggcggc ctacgcgggc gggctggccc
      301 cggtcctgtg cctgaccaag gcggacctcg gcgaccccga ggtcttcgct gcgcagtacc
      361 gcgaactcga cctgcccgtg ctcgtgacgc gccgggacca ggagccgacc gagctcgccg
      421 acgtgctcgc cggcagagtg tcggcgctgg tcgggcactc aggcgtgggt aagtccactc
      481 tggtcaacgc cctggttccg gacgccgcgc gagccgtcgg catcgtgtcc gcagtcggca
      541 agggccgaca caccaccgtc gccgcgctcg ccctgccgct gccggaggac aggggcggcg
      601 gctgggtcgt cgacacgccc ggcgtccggt ccttcggcct cgcccacgtc acgcccgacg
      661 acgtcccggc ggcgttcgac gacctcgcct cggcgatcga ggcctgcccg cgcggctgcg
      721 gccacctcgg gccgcccgcc gatccggagt gcgcactcga cgccctcgtg cagcagggcc
      781 tgctgcgtcc cgggcgcctc gccgcactgc gccgggtcct ggtggccctg cgttgacgcc
      841 acctggcact ctctccgcat gaccaccggg gaagagtcgt ctccgctgcg catcgggacc
      901 gcggagcgaa ccgcggcgat gaaggcgctc gacgagcacc tggcggccgg ccggctcggg
      961 gtggaggagt acgccgaccg ctctgcagtg gcagccaacg ccgtcgtcgc atccgagctg
     1021 gccgagctgt tcaccgacct gccggagccg cacccgaagc taccgggcac ggcctcgacc
     1081 gccccgctga cagcgccgct gcccgtcgtt gcaccgccgg gcgaggtggc cacgcagagg
     1141 ggtggcttcc tcgacggctg ggggccgcgg ctcgtcagcg tcacgccgtt cctcgcggtg
     1201 gcgttgttcc tcatcacccg gcagtggtat ttcttcctgc tcatccccgc gatgggcgcg
     1261 ctcgtctacg gcgggggcgt cggccgcaag gacgacgacc gcgaccgcga cgagcgtcgc
     1321 cgcgagcgca accagcgtcg ccgcgaccgg cgctgaccgg cagcgcggcc gcggccaggt
     1381 cgtagctcaa cggcctgctt cggtcgctca gctctccggc ggcgcagctc cgactgcgaa
     1441 ccgggcgacg tcggccacct cgggctgcag cgcctcggcg tgcgccgggt cggccacgtc
     1501 gagcgcgacc tccgcacgcc caccgcgccg ccggaggccc cacgtcccca ccacggcgcc
     1561 gtcgaccgtc gcgcaggccc gcagcattcc cccgcccggc tgtacccggg accgcaggtg
     1621 ctccggcacc gcgaacgagc ggtcggccca gccgagcagg taggggtcga acgggggcag
     1681 cagccggggt ggcagcgccg ggagcgcggc cacgtcgcgc tccgcgacgt cggcgaggcc
     1741 gtcgacgcgc tcgaccagcg cggtaccgag ccccgacatc ccggctcgcg cgtcgcgaag
     1801 cggcaggccc gaccacgccg cgagatcggc ggccgtggcg ggcccgtgac ccaggaggta
     1861 gcgacgggcc agctcgacga cggccacatg acgatcgatc ggctcagcgt gccggcctac
     1921 agcccggacg agcagctcgc ccgatgtgcc cgccggcacg agcaccgcct gcccgtgcag
     1981 tgccgccagg cgcacgcagt ggtgcacggc ctggccgaca accggcaccc cggcggccgc
     2041 cagccggtcg gcgagctccg cgcgagaccg ggccgctccg caggccagct ccgcgccgat
     2101 cacctgcagg gccgtgtcga cctgccggtc ggtcacgccc tcctcggcca gcctccgtcg
     2161 ggactcgacc acgcccgccc gcgcggtgag cccgagcagc catccgtggt cctcggtgcg
     2221 caccaggtgc agggttcccc gtaggagcca cgcgaccacg accgagccgt cgtcaggcgt
     2281 ggcgtccgcg gcggcgaggc cgcaggtgcg cgcacgcaga gccagccgcg ccgacctcca
     2341 gtcctgcgcc tggatgccga cgagcaggcc cacgacgtcc cgcggatccc gcacgcccgg
     2401 cgtgaggagc tgcgccctgc cccgctgcgc gcgcaggccg acccgcccgg ctccgcccgt
     2461 cagcccgact cccgtcagcc cagctccacc agggccgcga cctctgacgg gccgtaccaa
     2521 gccagctcgt ggtcctccgc ctcgccgacg aggaactccg cgtcgagatc gcccatgtcg
     2581 gccgcgtcga ccgcgccggc agcctggcgc acggcgtact cggcctcgtc gttgtccacg
     2641 tgcaccgcgg caatggtctc gaacggcacc ccgcccgcga tccggaccgc gccgtcgtcg
     2701 aggtcgggcc gcagggtgac gttctcgagg tcggcggcga tgaccacccg ccgcgcgggc
     2761 gtaccgtcct cgcccagccc gaactccacg gcgagcagcc gcagcgacgc ccgggccgcc
     2821 tcggtcaacg cggcatactc gagctcctcc tcgtcgccgc tcgtgtagga ctcacgcagc
     2881 ttcggggtga gcgcgaacgc cgtgccaccg atcgggtcga cgctgccgtt cttcaccagc
     2941 gtcctgagca tcggccaggt ggcgggtacg tagacgcgca tcgcgctcat caacccaccg
     3001 tcgccatgag ctcgtcgagc gactcgacga ccatgccggc gagcacgtcg atgtcggaca
     3061 tcccgtcccg gtcgccgttg aagccgtagt agacgccacc gtcgtaagag gtgagcccga
     3121 tcgagagcgc ctggcccttg gccagcggca ccaccgggaa catctcgagc atccgggcac
     3181 cagcggcgta gagcgggaac tgtggtcccg gcacgttcgt caccaccaag ttgaagatcc
     3241 gcttcgagaa cccgctggca gcgcgtgctc ccagggcgtg cagcgtaggc ggcgcgaagc
     3301 caccgatgcg gaccagggtg tcggcaccga ccgactggcc ggtggcggtg tgctcgcgca
     3361 tcgcgtgggt gacgtggtgc agccgcacga ccgggctggg ctcaccgacc ggcaggtcca
     3421 cgaggaacga cgacacgcgg ttgcccagcg acccggccgc agccgagctg ggcacgtccg
     3481 cttcgccgcg caccgacagc ggcaccatcg cccggaccga cgacgacggt gtgaccggct
     3541 caccacgcga gagcagccag ttgcgcaggg caccggagac gacggtgagc accacgtcgt
     3601 tgaccgtgca gccgtgcgcg gcccgcaccc tgcggtagga gtccagctgg gtgcgcgcgg
     3661 tcgcgaagcg ccgctgggtg gagatgtcga cgttgagcgg cgaccccggc gcccgcctgc
     3721 tcgccgtgcg tgcgatcgag aagagcttgc ctgcggtgcc ggtgatcttg ccgacggtgg
     3781 ccaacgcgtc gccggccgcc acgcggacgt tgtcgacgat ctcgcccggc cgggccacgg
     3841 cctcggcgac ggcaccgagg acgagctggg cgtgggtggg ctccggccgc ggcatccaca
     3901 gctcctcgac cggtgcgcgg gcccgccggc cgttctcgcc gaggagccgt aggtcgccct
     3961 cgccgggcgg cgagacgtcc aggatcacct gaccgatgtc gaacgcgctg atgccgtcga
     4021 ccatggcctg gtgggtcttg gtgatcatgg ccgtgcgccc gcgcgccacc ccctccacca
     4081 ggtacatctc ccacagcggg cgggtgtggt cgagcggacg cgacatcagc cgcgccacga
     4141 gctcggtgag ctgctcgtcc gaacccggtt tgggcagcgc cgaccggcgg acgtggtagg
     4201 ccacgtcgaa gtccgggtcg tcgatccaca ccggccgggc gaggttgccg ggtacgtacc
     4261 gcaccttctg ccggtagcgc ggtaccagcg cgatccgctg ctcgatcagc tccacgagcc
     4321 ggtcgtagtt gaatcctccc agcggccccg ccgaccgcgg cctccggaag gtcgacaccg
     4381 cgccgacgtg catcggtgtg gtcggctgct ccaggtacag gaatgaggcg tcgagtgcgg
     4441 agagccgggg aggcatgccc cagatcctag ggtggcaccc tgagaccgtg cggaacgtga
     4501 gcggagcgcg cggtgtcccg gagccggctg gccgttcccc gaaggacacc ttcatcaccg
     4561 tctacggccg gaagccggtg ctggaggccc tgcacgacgg gcagctgcgg gtggacaagg
     4621 tcatcatggc cgacgggttg cagggcgagc cggtgcgcgc catcaccgcg gctgcccgcg
     4681 accgtggcgt gccggtacag cgggcgtcgg cgcaccgggt gaaggtgctg gccggcaacg
     4741 gtcgccacga ccagggtgtg ctggccgacg tcgttgcgcc ccggatggcc cccgtcgcgg
     4801 agttcctggc cgagctcgca cccggcgcgc ccgcgtcggt gctggtgctc gaccggctga
     4861 ccaacccgtc gaacgtcggc atgatcctgc gcagttccac cgccgcgggt ctcgacggga
     4921 tactgctccc ccgccggggc gtgccggcga tcgacccgct ggtcgtcaag gcctcggccg
     4981 gtgtcgcctt ccgggcaccg gtgctgcggt ccggcactgt ggaggaaggg tgcgccgagc
     5041 tggctgcggc cggtttcacc gtgtacggcc tcgacgcggg tggcgcgtcg ctgctggagg
     5101 cggagctacc ggcgcgggtt gcgctggtgc tgggcaacga gaccgacggg ctgtcggagt
     5161 cggtgcggtc gctcgtcgac ggtctgctcg cgattcccat gcacggcggc gtcgagtcgc
     5221 tcggcgtggc cagcgccggc gccgtcgcgg cctttgagct cgcgcggcgt gcccgctgac
     5281 acaccccccg tacgcgggga gccggagttc taggctggcc caatggttca gcgcaggctt
     5341 ccccgcccct ccgagctccg cccgctgctg cggccggcgc cgttcgtgcg tgacgccgcg
     5401 gaacgacggc tgttgcgggc cgcgagcatc gcggacctgc gggcgatcgc ccgccgccgc
     5461 acgccgcggg cggtgttcga ctacaccgac ggcgcggccg acggtgagct gtccctgaac
     5521 cgggcccgcg acgcgttctc gcgggtcgag ttcacgccga cggtgctgcg tgacgtctcg
     5581 tccgtcgaca ccggacgcga catcctcggc cggcgctcga acctcccgtt cgccttcgcc
     5641 cccaccgggt tcacgcggat gatgcaccac gagggcgagc gggcagtggc ctcggtcgcg
     5701 cagaaggtgg gcatcccgta caccctctcg acggtgggca ccaccaccat cgaggaggtg
     5761 gcggcagccg cgccggacgc ccggaagtgg ttccagctct acctctggcg cgaccgcgcc
     5821 cccgcgaagg acctcgtgca gcgcgcggcc gacgccggct acgacacgat catgctcacc
     5881 gtcgacaccc cggtgggcgg cgcccggctg cgggacgtgc acaacgggct caccgtgccg
     5941 cccgccctgt cgctgaagac cttcgtggac ggcgcccgcc acccgcactg gtggttcaac
     6001 ctgctcacca ccgagccgct gacgttcgcg agcctcgagt cctccgaggg caccgtcgcc
     6061 gaactgatca accgtgtctt cgacccggcg ctgacgatga acgacgtcga atggctgcgc
     6121 gacacctggc cggggaagct cgtggtcaag gggatccaga gcgtcgagga cgctcgccgc
     6181 gtggtcgacg cgggcgcgga cgcggtgctg ctgtccaacc acggcggccg ccagctcgac
     6241 cgggccccgg tgccgttgga gctcgtggag cccaccgtgc aggccgtggg ggaccgcgcg
     6301 gaggtgctgg tggacaccgg gatcactcac ggggccgacg tactggcggc gatcgcactg
     6361 ggcgccaggg ccgcgctcgt cgggcgcgcg tacctctacg ggctgatggc cggaggccag
     6421 ctcggcgtgg agaaggccgt gacgatcctg gcaggcgaga tcgtccgcac gttgcagctg
     6481 atcggcgtca acagcgtcga cgagctgcgc ccctcccacg tccgacttcg tccgaacggc
     6541 tgagcccggc gtgcgacggc agcgcagcag ggccatggtg cagccatggg tttcatggac
     6601 aaggccaaag aactgctgaa ccggcacgac gacaaggtcg accagggcct cgacaaggcg
     6661 ggtgaggctg ccaagggccg gttcgccggg cacgagggcc agctcggcca gggcgtcgac
     6721 cacctcaaac gcatgacagg tgcgggggac accacagagc gggcgcccgg cgagccgcca
     6781 cccggcaccc cgccgcaggg gcccgctccg gaggacgcac ggcccgataa ccccaccggg
     6841 ggctacgagc cggcacagga ggtgccgggc ggaccacagc cggacgtgcc cgccggcccc
     6901 ggcaacgagc cgccgcagcg ctaggacggc ctcgcccggt ttggtccgag ccggatcggt
     6961 ccaccccgtc gccatggcag acgacagacg cgacgaccgg tccggccccg gggaggaggg
     7021 gttcctcgag agcctcctga gggacgcgac gattaccggg accgaggagg acccgccccc
     7081 accgccggcg gagccccagg tcgctgcccc ggagacgacg aacgacccgg acgacgacac
     7141 cgacagctcc tgacgcgcct cgtcagggcg gtggcaggtc cagcaggatc tcgacctcgc
     7201 cgatcgtggt gaccacgtcg cagtggtgta ccaccaccgc acccgctttc cgtgcgcccc
     7261 ggacgttgcc ggcatggtcg tcgaccacga cgcactgctc gacgggcagc cccagcatcg
     7321 ccgcgacgcg ccggaagatc tccggatccg gcttgcgcac cccgagcgcc gggccgagca
     7381 ccaccgcgtc gaacgtggtg gcgcactcgt cgggaaccgt gcgcccgtcg gacaccagcg
     7441 cggtggccac gcccgccgcc cgggcccgcc ggaccagcgc cagcacctcc ggcccgtccg
     7501 tgagcactcc ggcgtagtcc atcagcagcc cgcgcagcgg caccgcgggg aggctaccgg
     7561 ctccactgcc ggtagcccca gggtgtttgc cctagcctgt gagcatcttc tgcaggtcgt
     7621 ccagcacgag catcgcgccg gagggcccga gaccaaggaa ccagacctcg tcgtcgacga
     7681 ggcgcacgtg cccgttctgc accgccccga gccgggtcca gagctctccg tggacgacgg
     7741 cgccctgttc ggtggcctcc ggcggcccgt agctcgagta gaagacccag tcgccggacc
     7801 cgccgcgccc gagcgtggtg acgtccttca tcgctccgtg cccgcctcgt cacccaccgc
     7861 ggcgagcacg gcgagcaggt aggcgcggat gagctcggcg tcccccggcg caggagcagg
     7921 cagccgggcc cggaacgccg tcacgacgtc cccgtgcacc tccgccaggc gcgcgcgccc
     7981 gctcggcgtg atccacagcc gcaccacccg tcgatcggcc gggtcgcgct cgcgcgccag
     8041 ctcgcctgcg gcctccaggg cgtccaccac cggggtcagc gtggcgggag cgacgccgag
     8101 cttcccggag agttctcggt gggacaccgc accggtgtcc gcgaggactc ccagcgcccc
     8161 catcgcggtc gtggtcagcc cgtgcgcggc gatcgtgcgg ctggtgtacc gcaccaccgc
     8221 ctgtcctgtc cgcgacagca gtctgaccag cggcatcagc tgcgggtcgg gctcgaagaa
     8281 gccgtcggtc accggcagta cggtacggga tcttgatcgt cagcggccgt accgtccgtc
     8341 ggctgatcaa tccatcccga cctcgcgaca ctgcaccggc cagcaccctc gctgcgtgac
     8401 cacgacgctg accgcgactc cccgcccgac tcccgccacg tcgtcaggag cagtccgcac
     8461 ccaccgcgca ggagcgcccc gactcaggcg gatggcctac gagcccgaac cgggagcgac
     8521 tgccgcggtc ccgatccttc ctgttccccc gccgccgacg ccctcggcgg cgctcgacgc
     8581 ggcggccgaa ctcgccgaag cgcgtcacag cgtcgggcgg atcctccggc tggcgttgga
     8641 ggtactcgac ggccgcaggc cccccgtgca gctcaccggc cacgtcgccg cgcccgtgct
     8701 gcgctactgg cgtgcggccg cgcagcagcg gcgcgtgtgc tcccctgccc ggttcagccg
     8761 gatgcgactg tgcctgccgc gacccggcat cgcggaggtc gcagtggcgt gcgcgatgga
     8821 cggccgcgtg cgcgcgctcg cagcgcggtt cgagcgtgcc gacaacaccg gaaccggctg
     8881 gcggtgcacc gcgctgcggc tgggctgacc gagactgccg gggtcagggg cgggccgcag
     8941 tgggcgatcc gtggcactgc ttgtacttcc gacccgagcc gcacgggcac gggcggttgc
     9001 gcgacggctc gcccgaaccg gaaacggtgg cctcgccacg gctgccgtcg cgggccccgc
     9061 cgttgcccga ccgcgcccgc atggcgctgc cgtcctccgc cggaccgctg tagcgcagct
     9121 ccgtcgggcg ggaaccacgg aacgccggcg ggaggacgtt cggggcggtg accggctccg
     9181 cctccgaggg ccgagccgga gcgccctgcg gcgcagcacg ccgcggggcg ggcgactgct
     9241 gctgcaccgc tgcgggctgc gctgccgcct gccgcacggg ggcctgccgc accggggccg
     9301 cctgcgtact ggccgccggg gccggctggg ctgcggcggc gggctcctgc acgacggcgg
     9361 gctgggcggg ctgtacctgc gcgttgaaca ggtacgagac cgtttcctcc ttgatgccct
     9421 cgagcatcgc ggagaacatg tcgaacccct cccgctggta ctcgatcagc ggatcgcgct
     9481 gggccatcgc ccgcagcccg atgccgtcct tgaggtagtc catctcgtag aggtgctcgc
     9541 gccacttgcg gtcgatcacc gtcagcagca cctgccgctc cagctcccgc atcccgccga
     9601 gcggggcgat gagctcgtcc acctcggcct cgcgcagggc gtaggccgcc cgcgcatctg
     9661 cgagcacggc ctcggcgagc gtctccttcg tcaggtcgcc ggggtcggac agcccgttgt
     9721 cgtccttgcc gtcggtgagg ctctcccacc ggataccggg ggtgtagagc gtgccgagcg
     9781 ccgtccacag cttctcgagg tcccagtcct ccgcgtagcc ctccgcggtg gcaccgtcga
     9841 tgtacgccgt gacgacatcc tcgatcatgt gctcgatctg ctccttgatg tcctcgcccg
     9901 cgaggacccg gcgccgctcg tcgtagatga cggtgcgctg cttgttcagc acctcgtcgt
     9961 acttgagcac gttcttgcgg atctcgaagt tctgctgctc gacctgcgtc tgggcactgc
    10021 ggatggcctt ggtgaccatg cc
//
LOCUS       DASRSC010000070        12293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1333533,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000070 DASRSC010000000
VERSION     DASRSC010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12293
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333533"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(265..585)
                     /locus_tag="VFE39_03445"
                     /pseudo
     CDS             complement(265..585)
                     /locus_tag="VFE39_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
     gene            complement(744..1052)
                     /locus_tag="VFE39_03450"
     CDS             complement(744..1052)
                     /locus_tag="VFE39_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HET6257686.1"
                     /translation="MRWRRVSVRGPSMSPTLRDADVVLARFGSAVRSGDVVLVRWSAR
                     PGQLSVKRAALPSGTGWWVLGDNPYGSTDSRTLGAAEVLAVVGWRLWPRPGRLRGPVE
                     "
     gene            1144..1554
                     /gene="sodN"
                     /locus_tag="VFE39_03455"
     CDS             1144..1554
                     /gene="sodN"
                     /locus_tag="VFE39_03455"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HET6257687.1"
                     /translation="MLSRILRPRLEATAHCDLPCGVYDPAQARIEAESVKAIQEKYQG
                     NQDPEFRTRAIQIKEQRADLVKHHLWVLWTDYFKPPHFEKYPELHELFNKATKKAGGG
                     ADGAKSSMDPATGQELLDLIAQIDKIFWETKSAA"
     gene            complement(2686..3042)
                     /locus_tag="VFE39_03460"
     CDS             complement(2686..3042)
                     /locus_tag="VFE39_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6257688.1"
                     /translation="MYARDDIASALQWQRLPPGDHVDAAVNSFRMLADPTRLRLLWLL
                     CGGEQDVSALAAASGAARPAVSQHLAKLRMTGLVATRRDGRRVLYRARGGHVRRLLTE
                     AMEAADHIVTGAPEHG"
     gene            3062..4063
                     /locus_tag="VFE39_03465"
     CDS             3062..4063
                     /locus_tag="VFE39_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET6257689.1"
                     /translation="MHSSPQCGHGHTHAGPVRRVWSAARHVVVPHSHDPADRVDPAME
                     ASRAGMRALQVSLAVLGATAALQAVVVVASGSAALLADTLHNVADALTAVPLGIAFLL
                     ARRPATHRFTYGYGRAEDVAGIVVVLVILGSAALAGYESLRRLADPTDVRFLGAVAVA
                     GLVGAAGNEIAAQVRIRTGRRIGSAALVADGLHARTDALTSLAVLLSAGGAALAWRWA
                     DPVVGLAIAAAIATVSWSAARHVLARLMDAVDPGLVERATAALQDVAGVVGVDRVRLR
                     WIGHTLRAEVDLSVPPGLNIERAHTIAHAAEHELTHAVPRLTAATVHAHPARNWVSR"
     gene            4074..4667
                     /locus_tag="VFE39_03470"
     CDS             4074..4667
                     /locus_tag="VFE39_03470"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase"
                     /protein_id="HET6257690.1"
                     /translation="MTSGRVFLLRHGETEWSLSGKHTGRTDIPLTDRGRGLAKAAGEL
                     FRRLRGTDPPALVLTSPRSRARVTAELAGLEVDRVDERLAEWDYGECEGRTTTEIRET
                     IPDWTVWTHSCPGGETPEQVERRADSVLADARAELDRGDVVLVGHGHFSRVLLTRWIG
                     LPATHGVHFAMEAPSWAVLGDERGVPRLDHVNLRALA"
     gene            complement(4714..4848)
                     /locus_tag="VFE39_03475"
     CDS             complement(4714..4848)
                     /locus_tag="VFE39_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257691.1"
                     /translation="MGELRERCDVVETHVSNIPAKLGLNNRVQIALLVHDAALSDDPQ
                     "
     gene            4971..5468
                     /locus_tag="VFE39_03480"
     CDS             4971..5468
                     /locus_tag="VFE39_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6257692.1"
                     /translation="MTDRVRGVRPEDVPTVVALVHELAEYEREPQSCTLTDAQLAAAL
                     FGPAPALFGHVAEVVGPDGRAEIAGVALWFLNFSTWRGLHGVYLEDLYVRPNRRGRGL
                     GRALLATLAGVCAERGYARLEWSVLDWNEPSIRFYRAIGAVPMDEWTTFRLDGDALDA
                     LAKES"
     gene            complement(5465..5884)
                     /locus_tag="VFE39_03485"
     CDS             complement(5465..5884)
                     /locus_tag="VFE39_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257693.1"
                     /translation="MRAEEPAAARPPRQVQLAGVLVGVQGLLAIAFAAALGMRSLAPG
                     VPVGSVLGEAAYFVVIGVALLAVGAGLVTGRRWARTPAIVTQLLLLPVVYSLIGPSRQ
                     LVLGIVAGAFVVTTFMLLISERSREWSMGLDLPDARG"
     gene            6512..6763
                     /locus_tag="VFE39_03490"
     CDS             6512..6763
                     /locus_tag="VFE39_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET6257694.1"
                     /translation="MDWRHRAICRDEDPELFFPVGTSGPALLQIAEAKTVCRRCPVVT
                     ECLTWALESGQDAGVWGGMSEDERRALKRRNARTRARTA"
     gene            complement(6931..8424)
                     /locus_tag="VFE39_03495"
     CDS             complement(6931..8424)
                     /locus_tag="VFE39_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HET6257695.1"
                     /translation="MSTLSELLAEHTQLAGDAVDHLQRVVAEWQLLADMSFADFLLWM
                     PLADESQRTGLTEFLCVAQARPTTAPTAHPEDVVAARVMAADNPQLRRAVVEGRICRE
                     ENPRWHLEVPVRRETIPVRYGGAVVAVLSRDTNLAMPRVPSPLEIAYLGSASDLCQMV
                     ADGTFPPRAALAGADTSPRAGDGLVRLDAHGLVAYASPNALSAYHRMGHASDLVGLSL
                     IQATRELVHDPFDAAEVAARIGAALDGRHSLRMEVHARGAVMLVRALPLRPRGDAAGA
                     LVLVRDVTDLRRRDMALLSKDATIREIHHRVKNNLQTVAALLRLQARRTAIPEARRAL
                     AESVRRVNSIALVHESLSVSVAERVDLDELVDKVLPAIGDGATAEFRAKVRREGSFGT
                     LPAALATPLVLVLTELVHNALAHAFEPGRAGEVVVSAQRSARALDVVVADDGRGIPPG
                     FDLETSNGLGLQIVRTLLDSELDSALQVRPRAGGGTEAVIRLSLRAR"
     gene            complement(8696..9040)
                     /gene="rsrA"
                     /locus_tag="VFE39_03500"
     CDS             complement(8696..9040)
                     /gene="rsrA"
                     /locus_tag="VFE39_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HET6257696.1"
                     /translation="MSCGNHHDTDCSEVLAEVWLFLDHECDESRRALLAQHLEECGPC
                     LAEYGIDEKLKKLLASKCGGEHAPDDLKSRLRGQIRVAVLEQADVTVESGPDGTTTTV
                     EVRSSARLEQHG"
     gene            complement(9037..9696)
                     /locus_tag="VFE39_03505"
     CDS             complement(9037..9696)
                     /locus_tag="VFE39_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6257697.1"
                     /translation="MPGTEQAADAATTNGREVEQEQWQESAEERAARFERDAMPLIDQ
                     LYGAALRMTRNPADAEDLVQETYLKAYSAFRTFRAGTNLKAWLYRILTNTYINGYRKR
                     QRQPLQAATEEISDWQIAQAGEHTSQGLPSAEMEALDRLPDNDVKVALQQLPDDFRMA
                     VYLADVEGFAYKEIADIMGTPIGTVMSRLHRGRRQLRDMLTDTARDRGFLRGHQEEVS
                     S"
     gene            9913..10392
                     /gene="ybaK"
                     /locus_tag="VFE39_03510"
     CDS             9913..10392
                     /gene="ybaK"
                     /locus_tag="VFE39_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HET6257698.1"
                     /translation="MAGKGTPATALLTKQGVEHRLHSYTHGSGQAYGPEAAELLRLDP
                     ARVFKTLVAEVDGTLTVGIVPVLATLDLKALAAAVAGKRAKMADVALAERATGYVAGG
                     ISPLGQRKRLPTVLDASAERLETLFCSAGRRGLEVELAPRDLARLTGARLAPIAAFS"
     gene            10455..10664
                     /locus_tag="VFE39_03515"
     CDS             10455..10664
                     /locus_tag="VFE39_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257699.1"
                     /translation="MTGTITDRARDLRRAWGTRGSAPRVLPPHLTRAVYGLWIAAFAL
                     KILGSTWDVSWHFRWLRDDLAPPTC"
     gene            complement(11320..11934)
                     /locus_tag="VFE39_03520"
     CDS             complement(11320..11934)
                     /locus_tag="VFE39_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HET6257700.1"
                     /translation="MSTEIGTPHGPARITRHDADGDATAVLLLGHGAGGGVGAPDLLA
                     AAGTAVAAGVHVVLVEQPYRVAGRRAPAPAAQLDAAWLAVVEHVRAELALPMLFGGRS
                     SGARVACRTAAAGGAVGVLCLAFPVHPPGKPEKDRLHELAAPTVPVLVVQGATDPFGR
                     PDPAPGHEVVVLRGDHSLKSDMPGLRTAVAGWLEDHFGAVLLRQ"
     gene            complement(12104..>12293)
                     /locus_tag="VFE39_03525"
     CDS             complement(12104..>12293)
                     /locus_tag="VFE39_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET6257701.1"
                     /translation="SDAVAALLRPPSEQLVANLELRPVSPLVNSVRNNGPELLDPLQP
                     EDVREPLQLDLLAGPNPK"
BASE COUNT         1769 a         4432 c         4330 g         1762 t
ORIGIN      
        1 tcggtgatca gcccatcggt ctcgttcgcc tcggcaacct gctcaccggc cgaaccctca
       61 ccagaatggt gtccgagctc ttccacgtgg cccccagcgg tcatggcgtc tatctcaccg
      121 ggtcaacgac gccgattccc gtgcgatctg gacgaccgcc caaacggcct acaccacgtt
      181 gaggacaaga acccgctggt cagcacgctg cgtcaccggt gcagccatta gggggtgggg
      241 taggaaggaa gggtcaccta ctcgtcactg ctcgtagaat tcgccccggc tgcccgccgc
      301 caccgccggt gcgacacgct ggtcggacgg gctcggcacg atccgggcgg ccgcgagatc
      361 ttcggtcgcg acgaagtgga tcgcgtcggc ggctccgagc ttcatcgatt cgatgatccg
      421 ccgcgccccg gcgtcgagcg ggccgcggaa gacaccgggg aacgcgagca cgttgttgat
      481 ctggttgggg acgtcgctgt gccccgtggc gatgatggcg gcgtgcctcg ccgccacagc
      541 cgggtgaatc tcgggatccg ggtgggaaag ggcgaacacg atcgcgcgtc gctcgctcgc
      601 cgtgcaccgg agggtcgcgg gtatcggccg cctcacggtg cggacggcct ctcgtgcagc
      661 ggccgcggga tgacgcggtc cggaccggcg aggttcggct gtcgggtacg cggacgaccc
      721 tggtgccgtt tccggcacgg cgatcactcg acggggccgc gcagccgccc tggccgcggc
      781 cacagccgcc agcccaccac cgcgagcacc tcggcggccc cgagcgtgcg cgagtcggtg
      841 gagccgtagg ggttgtcgcc gagcacccac cacccggtgc ccgaggggag cgccgcccgc
      901 ttcaccgaca gctgccccgg acgcgcggac cagcggacca gcacgacgtc gccggaacgg
      961 acagcggacc cgaaccgggc gagcaccacg tcggcgtccc gcagcgtcgg cgacatcgac
     1021 ggaccgcgga cggacaccct ccgccagcgc acgcgtaccc cccttgccgc cctgctgcga
     1081 tctggctccc agagtagtgt cggaaacgcc agaacgatca ccaatcaggg aggacccatg
     1141 cggatgctgt cccgcattct ccgcccgcgg ctggaggcca ccgcgcactg cgacctcccc
     1201 tgcggcgttt acgacccggc tcaggcacgg atcgaggccg agtcggtcaa ggccatccag
     1261 gagaagtacc agggcaacca ggacccggag ttccgcacgc gggcgatcca gatcaaggag
     1321 cagcgcgcgg acctcgtcaa gcaccacctg tgggtgctgt ggaccgacta tttcaagccg
     1381 ccgcacttcg agaagtaccc ggagctgcac gagctgttca acaaggccac caagaaggcc
     1441 ggcggcgggg ccgacggcgc gaagtcctcc atggacccgg ccaccggcca ggagctgctc
     1501 gacctcatcg cccagatcga caagatcttc tgggagacca agtctgccgc ctgacccggg
     1561 cgctcgaagc ggcgctgacc cacgggagga cctcccggtt ggtcagcgcc gtttctcgtt
     1621 gttgggtggc cttccacggc ccgccgacgg cccagccgca gagctggggt gccgcttggc
     1681 tgccaggcac tcggagacac acggcgacct gagccgtccg gttcgcgtgc tggttcagta
     1741 gcccgcagcc gtcccaccgt gcgcatttgc gtcgcgcggc ggttgtcact cggctagtcg
     1801 ctcacgacaa ctgctcggcg agggcatgat ccttgttgtc gacagtgccc gtcgaccata
     1861 ggcagcgagt tcggctctcg ctgatcaggc tgcggtgccg aacgggttgt cgatgatgta
     1921 tcgccaggta ccgcttcgac caacgtcggg cggcgtcggt ggcggtacct gcgatgcgca
     1981 caaacgctcc gtagctcgtg gagacgtgcg ttggcgacag ctcgttcatg cccgcctccg
     2041 tcgggcgacc gtggcggcgg aggccgcgcc gtggtctttt ccgtacatgg gtcgagggga
     2101 cggtccgcgt cgctcggcgc agaccgtgcg ccggcccggt gacgggggtg ccacgtcagt
     2161 gtctgcagcg gcagctcagg cacaaggccg tcggcgagga cgtcgcggag gtggcggggt
     2221 gggctgggcg ccccaggagg ggcgtagcac ggtcggctcg ggcgcggtcc gggcgacgac
     2281 acggtgcagc tcgccgaggc ccgtggcggt ggcttcgacc gccgaggaac tcagcagcac
     2341 gacgcggcgc aaacctcggc ggactgcccg ctcgagggtc gggcgcacga acggtacggg
     2401 gtctgccccc cggaccggca cgagaaggta gagccgttcg acgccgtcga acgctgcctg
     2461 ctgggtactg gggtcggccc agtcgaaggg gagcggctgg cgcggcgcac gtcagcgccc
     2521 gcggtgtcga ggagatcggt gaggcgacga ccggtgctgc cggtgacccc ggtgaccaag
     2581 acggtcatcg tccccgcccc ggcgagcccg cctgcgccca agaccgcctg gtctctcgtt
     2641 tcggccagtt ctctcgtccg atcgacaaga acaggacgat gggcgtcagc cgtgctcggg
     2701 cgcgccggtc acgatgtggt ccgccgcctc catggcctcc gtcagcagtc tgcgcacgtg
     2761 gcccccgcgg gcgcggtaga gcacgcgtct gccgtcccgc cgggtggcca ccagcccggt
     2821 catccgcagc ttcgcgaggt gctgcgacac cgcgggacgc gccgcaccgc tcgccgcagc
     2881 gagagccgac acgtcctgct cacctccgca gagcagccac agcaaccgca gccgagtcgg
     2941 gtcggcgagc atccggaacg agttcacggc cgcgtcgacg tggtcaccgg gcgggagccg
     3001 ttgccactgc agggcacttg caatgtcgtc gcgtgcgtac atgtgcacgt atggtgcctg
     3061 catgcacagc tcgccgcaat gtggccacgg gcacacccac gcaggtccgg tccgtcgggt
     3121 gtggagcgcc gcccgccacg tcgtcgtccc gcacagccat gatccggctg accgcgtcga
     3181 ccccgcgatg gaggcctccc gcgccgggat gcgcgccctg caggtctcgc tcgccgtgct
     3241 cggcgcgacg gccgccctcc aggccgtggt ggtggtggcg tccggatcgg cggccctgct
     3301 ggccgacacc ctgcacaacg tcgccgacgc actgaccgcc gtgccgctgg ggatcgcctt
     3361 cctcctcgcc aggcgcccgg cgacgcacag gttcacctat ggctacgggc gcgcggagga
     3421 cgtggcgggc atcgtggtgg tgctggtgat cctcggctcg gccgcactgg ccggctatga
     3481 gtcgctgcgc aggctggcgg accccaccga cgtccggttc ctcggggcgg tcgccgtcgc
     3541 cggtttggtg ggcgccgccg gcaacgagat cgccgcgcag gtgcgcatcc gcaccgggcg
     3601 gcggatcggg tcggccgcgc tcgtcgccga cggcctgcac gcccgcaccg acgccctgac
     3661 ctcgctcgcg gtactcctgt ccgccggcgg ggcggcgctg gcctggaggt gggccgaccc
     3721 cgtggtgggc ctggccatcg ccgccgcgat cgccaccgtc agctggagcg cggcccggca
     3781 tgtgctcgcc cgcctgatgg acgccgtcga cccgggcctc gtcgagcggg ccaccgccgc
     3841 actgcaggac gtggccggag tggtcggcgt ggaccgggtg cgcctgcgct ggatcgggca
     3901 cacattgcgc gcggaggtcg acctgtcggt gccacccggc ctcaacatcg agcgggccca
     3961 cacgatcgcg catgcggccg agcacgagct cacccacgcc gtcccgcggc tcaccgccgc
     4021 caccgtgcac gcgcaccccg cacgaaattg ggtgtcccgc tagggtcggg acggtgacat
     4081 cgggccgggt gttcctgctt cgccatggtg agacggaatg gtcgctgtcg ggcaagcaca
     4141 ccggccgcac cgacatcccc ctcaccgacc gcggccgtgg cctcgccaag gccgcgggcg
     4201 agctcttcag acggctgcgg ggcaccgacc cgcccgccct cgtcctcacc agcccgcgca
     4261 gccgcgcccg cgtcaccgcc gagctcgccg gcctggaggt cgaccgcgtc gacgagcgcc
     4321 tggccgagtg ggactacggg gagtgcgagg gccgcacgac caccgagatc cgcgagacga
     4381 tcccggactg gacggtctgg acccattcct gccccggggg ggagacgccc gagcaggtcg
     4441 agcgccgggc cgactccgtg ctcgccgacg cccgcgccga gctcgatcgc ggcgacgtgg
     4501 tgctcgtcgg gcacgggcac ttcagccgcg tgctcctcac gcgctggatc gggctgcccg
     4561 ctacccacgg cgtccatttc gcgatggaag cgccctcctg ggccgtgctg ggggacgagc
     4621 gcggcgtgcc gcgcctcgac cacgtgaacc tgcgggcact ggcgtgaacg caccgtcggc
     4681 gcggtgatcc gagaggagat gcgacggcag aactcactgc gggtcgtcgg agagagcggc
     4741 gtcgtgcacc agcagcgcga tctgcacgcg gttgttgagg ccgagcttcg cggggatgtt
     4801 cgagacgtgg gtctcgacga cgtcgcaccg ctcgcgaagt tcgcccacaa caccgcgggc
     4861 atcgtggact cgctggccct cggtgcctgg gctgcacggc gaaatccccg tcgctcctcg
     4921 tcggcgcgac cgaccggcac agcgatggtc gactagggtc ttcccgtgtg ctgaccgatc
     4981 gtgtccgggg cgtgcgcccg gaggacgtgc ccaccgtcgt cgcgctggtg cacgagctcg
     5041 ccgagtacga gcgcgagccg cagtcctgca cgctgaccga cgcgcagctg gccgctgccc
     5101 tgttcggccc ggcacccgcg ctgttcggcc acgtcgccga ggtcgtcgga cccgacggaa
     5161 gagccgagat cgccggcgtc gcgctctggt tcctcaactt ctccacctgg cgtggactgc
     5221 atggggtcta cctggaggac ctctacgtgc ggcccaaccg ccgcgggcgc gggctcggac
     5281 gggcgctgct ggccacgctg gcgggtgtct gcgccgagcg gggctacgcc cggctggagt
     5341 ggtcggtgct ggactggaac gagccctcga tccgcttcta ccgtgcgatc ggcgcggtgc
     5401 cgatggacga gtggacgacg ttccgcctcg acggcgatgc gctcgacgcc ctcgccaagg
     5461 agagttagcc ccgcgcgtcg ggcaggtcca gacccatcga ccactcccgc gaccgctcgc
     5521 tgatgagcag catgaacgtg gtgacgacga acgcccccgc gacgatcccc agcacgagct
     5581 ggcgcgacgg cccgatcaac gagtagacca ccgggagcag gagcagctgc gtgacgatcg
     5641 ccggcgtgcg cgcccaccgg cgtccggtca cgaggccagc gccgacggcg agcagcgcga
     5701 caccgatgac gacgaagtag gcggcctcgc cgaggacgct gcctacgggc acccctgggg
     5761 cgagcgaccg catccccagg gccgccgcga aagcgatggc gagcaacccc tgcacgccga
     5821 ccaggacgcc ggccagctgc acctgccgcg gcgggcgggc cgccgctggt tcctccgccc
     5881 tcacatggcc gagaataggc caggcgccac ccggcggctg cgggtcccgg ctgcggcggg
     5941 aatgctcggg ccggcacgcg cgttgcacaa gctcggtgag cagggtgaat gctggaaagt
     6001 ccgtagaccg accggatcat cctgcgaagc accaaagggt gattccggaa cgtgttcgcg
     6061 gcactacggg tcatcgtgtg agatgcgcag ctcgtcgcag ccgagcggcg gccttcactc
     6121 gtcagggtga ccggccgcgg ccattcaccg atgctcgcgg accgtcgagc cgataagggc
     6181 tcgcacctgg gcaaacgttg cttcatggtg ggtcacggac aagcgagtga tcgcgaggat
     6241 ctttctcgca tggtccttcg ggagtcgggg taacccgact ctgatgggcg ggacgaggtt
     6301 gatcgctcat ggtgacacgc cacgcagttg ggtacgcacg tgtaaccgat cgtggcgagc
     6361 gtcgttgcta tgagtgggtg agactgctca cctgagtgga cctcggccct tgaacaccga
     6421 gcgcgctcgt gaaacgcttc acaagcgcgg aaacaccgtg acggacgatc ggcgcgaccc
     6481 cgatcgcgca agtacaagga gccgagagac catggattgg cgtcaccgcg ctatttgccg
     6541 cgacgaggac cccgagctgt tcttccccgt ggggacgagc gggcctgcgt tgctgcagat
     6601 cgcagaggcc aagaccgtct gccggcgctg ccccgtggtc accgagtgcc tcacctgggc
     6661 gctcgagagt ggccaggacg caggcgtctg gggcggaatg agcgaagacg agcggcgggc
     6721 cttgaagcgc cgcaacgcgc gaacgcgcgc acgcactgcc tgacatacaa caggtaaaca
     6781 acctccccca ccgcgggccc gaggccggac tgcacccggc tccgggcccg cggtcgttcc
     6841 accgggcgcg ctcgggggca gctccggagg cggatcgacg gaccgggctg cgagcgacca
     6901 tcaccgaacc cctcgatcac gacctaaagg ctaacgcgcc cgcaacgaga gcctgatgac
     6961 ggcctcggtt ccaccgcctg cacgcggccg cacctgcagc gcggagtcca gctcggagtc
     7021 gagcagagtc cgcacgatct gcagtcccag tccgttcgac gtctccaggt cgaaccccgg
     7081 cgggatgccc cggccgtcgt cggccaccac cacgtccagg gctcgcgccg agcgctgggc
     7141 cgacacgacg acctcgcccg cacggccggg ctcgaaggcg tgtgccagcg cgttgtggac
     7201 cagctcggtg agaacgagca ccagcggcgt ggccagggcg gcgggcaggg tgccgaagct
     7261 gccctcccgc cgcaccttgg cgcggaactc cgccgtggca ccgtcaccga tggcgggcag
     7321 gaccttgtcg accagctcgt cgaggtccac ccgctcggcc acggagaccg acagcgactc
     7381 gtgcaccagc gcgatggagt tgacacgccg aacgctctcc gcgagcgccc gacgggcctc
     7441 cgggatcgcc gtgcggcggg cctgcaggcg cagcagcgcc gcgacggtct gaaggttgtt
     7501 cttcacccgg tgatgaatct cccggatcgt ggcgtccttg ctgagcagcg ccatgtcccg
     7561 gcggcgcaaa tctgtgacgt cgcgcaccag caccaacgct cccgccgcgt cgccgcgcgg
     7621 gcgcagcggc agggcgcgca cgagcatgac ggccccgcgg gcgtgcacct ccatccgcag
     7681 cgagtgccgc ccgtccagcg cggccccgat ccgtgcggcc acctcggcag cgtcgaacgg
     7741 gtcgtgcacg agctcccggg tggcctggat gagcgacagc ccgacgaggt cgctggcgtg
     7801 gcccatgcga tggtaggccg acagcgcgtt ggggctcgcg taggcgacca ggccgtgcgc
     7861 gtcgaggcgg accaggccgt cgcccgcccg agggctggta tccgcgccgg ccaaggccgc
     7921 ccgcggtgga aaggtgccgt cggccaccat ctggcacagg tccgatgcgc tgcccaggta
     7981 ggcgatctcc agcgggctcg gcactcgggg catcgcgaga ttcgtgtcgc gggagagcac
     8041 tgcgaccacc gccccgccgt accggaccgg gatggtctcc ctgcgcaccg gcacctcgag
     8101 gtgccagcgc gggttctcct cccggcagat gcgcccctcg acgaccgcgc gccgcagctg
     8161 cgggttgtcc gcagccatga cgcgcgccgc gaccacgtcc tccggatgcg cggtcggcgc
     8221 cgtcgtcgga cgggcctggg ccacgcagag gaactcggtg agacccgtgc gctgactctc
     8281 gtcggccagc ggcatccaga gcaggaagtc ggcgaacgac atgtccgcga gcagctgcca
     8341 ctcggccacc acccgctgca ggtggtcgac ggcatcgccg gccagctggg tgtgctcggc
     8401 gagcagctcg ctgagggtgg acacccgact cttcctttcg gtcgtggctc gtcgccccga
     8461 tcactccggt ccagtcaagc acgtggaaca ctcgacctac cgacgggacg aacaaccgtt
     8521 cgcggagaac gagggagctg ggagggaacc atgtccaagc gcgcacgcaa gcgccgcgac
     8581 cgcaagaaga gcgcggccag ccacggcaag cgacccaaca cctgatcctg atgtgcgaga
     8641 gcgccggggc acatcgcccc ggcgccctgt cgccgatccc ctcagctctt cgtgctcagc
     8701 cgtgctgctc gaggcgggca ctgctgcgca cctcgaccgt ggtcgtcgtc ccatccggac
     8761 ccgactccac cgtcacatcg gcctgctcca gcaccgccac tcggatctgc ccgcgcagcc
     8821 ggctcttcag atcgtccggg gcgtgctctc cgccgcattt gctggcgagc agcttcttca
     8881 gcttctcgtc gatgccgtac tcggccaggc acggcccgca ttcctccaga tgctgggcga
     8941 gcagcgcccg gcgcgactcg tcgcactcgt ggtcgaggaa gagccacacc tcggcgagta
     9001 cctcggagca gtcggtgtcg tgatggttcc cacagctcat gaggagacct cctcctgatg
     9061 tccacgcagg aacccgcgat cgcgcgcggt gtcggtgagc atgtcccgca gctgacgcct
     9121 gccacggtgc agccgcgaca tcaccgtgcc gatcggggtg cccatgatgt ccgcgatctc
     9181 cttgtacgcg aagccctcga cgtcggcaag gtagaccgcc atccggaagt catcgggcag
     9241 ctgctgcagc gcgaccttca catcgttgtc ggggagccgg tccagcgcct ccatctcagc
     9301 cgacggcagg ccctgagagg tgtgctcgcc cgcctgggcg atctgccagt cgctgatctc
     9361 ctcggtcgcc gcctgcagcg gttgacgctg cctcttccgg taaccgttga tgtaggtgtt
     9421 ggtgaggatc cggtacagcc aagccttgag gttcgtgccc gcccggaacg tccggaaggc
     9481 cgaatatgcc ttgagataag tctcctgaac gagatcctct gcgtcggccg ggttgcgcgt
     9541 catccgcagc gcggccccgt acagctggtc gatcagcggc atcgcgtctc gctcgaaccg
     9601 ggcggcccgc tcctccgcgg actcctgcca ctgttcctgc tcgacctcgc gaccgttggt
     9661 cgtcgctgcg tcagccgcct gttcggtgcc gggcaccctg ctcctctcgc gccgagtcgc
     9721 gcgagcggcg accccggggc cgacctccgt tgcggcggcc ggttcacggc tcgagcttac
     9781 gccgcccgat ccgccgtagg ccgccggacg cagcccacca tcggggcgcg gggtatccat
     9841 tactgcctga ctcacacctt tcacaacgcc ggtccaagtc aagtgattcc ccccgctcgt
     9901 taccgtgccc tggtggcagg gaagggaacg ccggcgaccg cgctgctcac aaaacagggt
     9961 gtcgagcacc gcttgcacag ctacacccac gggagcgggc aggcctacgg cccggaagct
    10021 gcagagctgc tgcggctgga cccggcgcgg gtgttcaaga cgctggtcgc cgaggtggac
    10081 gggacgctga ccgtcggcat cgtcccggta ctcgcgacgc tcgacctgaa ggcgctcgcc
    10141 gccgccgtcg ccggcaagcg ggcgaagatg gccgacgttg cgctcgccga gcgggccacc
    10201 ggctacgtgg caggcggcat ctccccgctg ggccagcgca agcgcctgcc gactgtgctg
    10261 gacgcctctg cggaaaggct ggagacgctg ttctgcagtg ccggccgccg ggggctggag
    10321 gtggagcttg ctccccgcga cctcgctcgg ctcaccggtg cccgcctcgc gccgatcgcc
    10381 gccttctcct gaggtcgccc ggtccggcga ggatcccgtt ccaggacggg ggacggcagg
    10441 cgatcatgtc gcgcgtgact gggacgatca ccgatcgcgc gcgtgacctc cggcgagcct
    10501 ggggcacacg cgggagcgct ccgcgcgtgc tgcccccgca cctgacccgc gccgtgtacg
    10561 gcctgtggat cgccgccttc gccttgaaga tcctcggctc gacgtgggac gtctcgtggc
    10621 acttccggtg gctgcgcgat gatctggctc cccccacctg ctgaacacga ccggcaccgt
    10681 cttggtgatc ctgctggtgg cgttccacag ctggaccggc tacggcgtcg accgggcggc
    10741 gctgcggatg atgcgggccg ggatcgcgat gttcctggcc gctgtgccga tcgacgtggt
    10801 caaccacgcg gtcaacgggc tcgacatcac agcgtggagc gtgtcacacg cgctgctgta
    10861 cctgggcacg gccgtgatgc tggccgggtg gttcgcggat ggtcgcgcta cggaacgggg
    10921 cgggtgcatg ctccgctgct cgtgctgctg tgggcgttcg tcctggagaa cgtgtggttc
    10981 cccgcgcagc agcaggagta cggcgtgctg gccgtcgcgg cctgggaccg cgaggcggcg
    11041 gacgcggagc cgatcctgct gcagttcgcg gccgacatcg cgttcctcgg cgcgctcccg
    11101 gtggtcctgc gcctgctgct cggcgccgcc ctggtgggcc tcgccgccgc cggcggagcc
    11161 gcgctgcagg ccacgtgggc cgcgctgccg ccgatcgccc cggcgggttc gtcaccggcg
    11221 cggtgctggc agccctctgg gtcgggacgg tgctgctggt ccgcagccct ccgtcggtgc
    11281 gctgaccgac gtcagcccga accatcctgg ggttcgcggc tactggcgca gcagtacggc
    11341 cccgaagtgg tcctccagcc agcccgccac cgcggtgcgg aggcccggca tgtcgctctt
    11401 caggctgtgg tcgcccctca gcaccaccac ctcgtgtccg ggagcgggat cgggacggcc
    11461 gaacgggtcg gtcgcaccct gcaccacgag caccgggacg gtcggtgcgg ccaactcgtg
    11521 cagtcggtcc ttctccggct tgcccggtgg atggacggga aacgcgaggc acagcacgcc
    11581 gacggcaccg cctgccgcgg ccgtccggca ggccacgcgg gccccggagc tgcggccccc
    11641 gaacagcatc ggcagcgcca gctcggcacg gacgtgctcc accactgcga gccaggccgc
    11701 gtcgagctgg gcggcgggag cgggcgcccg gcgaccggcc acccggtagg gctgctcgac
    11761 cagcacgacg tgtactccgg cggcgacagc ggtgcccgcc gcagcgagga ggtccggtgc
    11821 gcccacccca ccgcccgcgc cgtgtccgag caggagcacg gccgtcgcat cgccgtcggc
    11881 gtcgtgccgg gtgatccggg ccgggccgtg cggcgtcccg atctcggtgc tcacggccgc
    11941 accccgaagt gatccgcgcg attcgcgcgg gagcgccccg acgacggcgc tggtgtgcga
    12001 cccacggcga tcagcgcacg ctcccccggc cgtgatcggc gcgagcccca ccgaaagcgc
    12061 ccctgcgagg gccacggtgt gtggctcgtg gcgatcatcg ggctcacttc gggttcggcc
    12121 cggccagcag gtccagctgc agcggctcgc ggacgtcctc cggctggagc gggtcgagca
    12181 gctcggggcc gttgttgcgc acactgttga ccagcggcga caccggccgc agctccaggt
    12241 tcgccaccag ctgctccgac gggggccgca gcagcgccgc caccgcgtcg gag
//
LOCUS       DASRSC010000071         6006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1988352,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000071 DASRSC010000000
VERSION     DASRSC010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6006
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1988352"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFE39_03530"
     CDS             complement(<1..470)
                     /locus_tag="VFE39_03530"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET6257702.1"
                     /translation="MSAPPGPVSPAVMREVLGHFVSGIVVVTAAGPDGPIGFTCQSFA
                     SLSLDPPLVTFAPARSSSTWPRIREVGAFCVNVLAADHQELSVGFARSGVDKFAGVPW
                     QAAPSGAPILDGVSAWIDCTLWNEYDGGDHTVAVGLVSDLGADPARLPLLYYRGR"
     gene            566..2044
                     /locus_tag="VFE39_03535"
     CDS             566..2044
                     /locus_tag="VFE39_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HET6257703.1"
                     /translation="MATQLEQFRMTIGGKAVDALSGRTFESQNPYTGRPWAVVPDGGP
                     EDVDAAVAAARAAFEGEWGAQTGFARAALLRRLGDLIGENAERLARLEVNDSGKLYRE
                     MIGQLTGIGTWFHYYAGLADKIEGKQIPAGNPNYLVYTRREPVGVVAAITPWNSPLLL
                     MTWKLAPALAAGCTVVVKPSEHAPASTLAFAKLVEQAGFPPGVVNVVTGLSREVGAAL
                     AGHPGVDKVAFTGSTATGRAVAAAAAENINKVTLELGGKSPQIVFPDADLQAAANGLV
                     AGVFAATGQTCMAGSRLIVHADVHDELVRLVAERAARIRLGDPNDPGTEMGPVANRPQ
                     YEKVLGYLATARAEGATIAYGGAADEGLGGLFVRPTVLTGVTADSTVVREEVFGPVLA
                     ALTFTDEDEALKLANDTPYGLAGAVWTKDVHRAHRVAARLRAGTVWINAYRVVSPGVP
                     FGGYGASGLGRENGAAGLDEYLENKSVWVELTGGTRDPFTLG"
     gene            complement(2031..2987)
                     /locus_tag="VFE39_03540"
     CDS             complement(2031..2987)
                     /locus_tag="VFE39_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HET6257704.1"
                     /translation="MTEPVIIGSERSEAGLPAGMAVLRDGGSALDAVEAALRLCEDNL
                     SDHYVGTGGLPNARGEVELDASVMIGSTRAFGAVAAVRGYPHPISVARAVMEHLPQHA
                     LLVGEGAELFAAECGFSTAELLTDEARREWHDALSVTDESVEGENTRAGSGDVAYRLA
                     ALELVKRMAPHDGPWGTINVLALDGSGELVVGVSTSGYPWKYPGRVGDSAIAGAGNYC
                     DLRYGGAACTGRGELSIRATGARVVVDRLAAGDDPESACAAMLADAATLPDTFRAELR
                     TLALTPDGRHGGAAGQEGSTYSVMTAADAEVRFLPRRVVSPA"
     gene            complement(2984..4093)
                     /locus_tag="VFE39_03545"
     CDS             complement(2984..4093)
                     /locus_tag="VFE39_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6257705.1"
                     /translation="MTVTTPTPSVSNSRGDAVLTVSGLVQHFVGAHTWTDRLARRPAH
                     VVKAVDGVDLTLAAGETLGLVGESGCGKSTLSRAVMGLYQATAGEIRYGGGVVEGRPN
                     RVQRRTTQMVFQDPYSSLNPRMTVGQTIGELLRYHQIVPRDQVTARSRELMDLVGLSH
                     DALGRTPRQFSGGQRQRIAIARALALEPKVLIADEPVSALDVSVQATIINLLLDLKES
                     LGLSMIFVSHNMAVVRQVSDRIAVMYRGRIVETGATDELFTDPVHPYTQLLLSSVPRM
                     LTRVHGARAEGTADPLAVVDGEPEADESPFAAHTIEGEGTGCRFRPRCPVAIAACAAE
                     PDLLPLPDDPVRSAACWHAAERPSSTQLTAKGRAR"
     gene            complement(4090..5175)
                     /locus_tag="VFE39_03550"
     CDS             complement(4090..5175)
                     /locus_tag="VFE39_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6257706.1"
                     /translation="MIGVRRESEQLAARPVAGGAHALLEVHDLEVEIPLPHGRLHAVR
                     GLSLQVDAGEAIGVVGESGSGKSLTLRALLGLLPHPARLTGGSILLDGVDVTGLPARR
                     RQQVLTEAMSMVFQDSLTALNPVMRVGDQIAEVPRRRFGMSRSEAHSRAVDLMDRVGI
                     PDPEKRFRAYPHELSGGMRQRICIAIALSAAPRLILADEPTTALDVTIQAQVLDLIDT
                     LRRDEQLGLVLVSHDLAVVSQTCSRVYVMYAGRVVESGEARELLTGPRHPYTFALLRS
                     VPDPDMPVHRLLTIAGQPPDLTELHRGCSFAPRCPFAQEQCTAEAAEPALVAVSGGRH
                     NSACLRWETAPEWDGAPGPADTLESRS"
     gene            complement(5172..>6006)
                     /locus_tag="VFE39_03555"
     CDS             complement(5172..>6006)
                     /locus_tag="VFE39_03555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6257707.1"
                     /translation="TDAATPRRRRGSVNVTLIAGLTLFLLIVLAAICAPLLTSADPIA
                     QDLLQARLPPGSPGHLLGTDQLGRDVLSRLLYGGRTDLQVGVLAVVTPFVAGTVLGLL
                     TGWIGGWFDLVVMRVVDVVVAFPFFVLVIALVFALGAGTGSIYVAISMVGWVAYCRIV
                     RGEVLRAKEQEYALAAQASGLPTWRILFRHLLPNVVVQAVVYSMSDIVLTILAIVTLS
                     YLGLGVPPPTPDWGSMIQDGQQFLLTQWYIATIPGLAVVLVGLALALVADGLVERFGQ
                     R"
BASE COUNT          894 a         2158 c         2170 g          784 t
ORIGIN      
        1 cggccgcggt agtacagcag gggcaggcgg gcaggatcgg caccgagatc gctcacgagg
       61 ccgacggcga ccgtgtggtc gcccccgtca tactcgttcc acagtgtgca gtcgatccac
      121 gcgctgacgc cgtcgagaat cggtgctccg gacggagccg cctgccacgg caccccggcg
      181 aacttgtcca cccccgagcg ggcgaacccg acgctgagct cctggtggtc ggcggcgagg
      241 acgttgacgc agaacgcgcc gacctcccgg atgcggggcc acgtcgacga cgaacgggcc
      301 ggcgcgaagg tgaccagcgg agggtccagc gacagcgatg cgaacgactg gcaggtgaac
      361 ccaatgggcc cgtccggtcc ggcggccgtg accaccacga tgccggacac gaagtgcccc
      421 agcacctccc gcatgacggc cggcgagacc ggaccgggtg gtgcgctcat gtcctgagcc
      481 taggacgccg cctgccgtga cgtcggcgtg gcgtagccca catcgacgat ggaccgtgac
      541 ggggtccggc tcgtaccgtc ggggcatggc gacccagctc gagcagttcc ggatgacgat
      601 cggcgggaag gccgtcgacg ccctgtccgg ccggacgttc gagtcgcaga acccctacac
      661 cgggcggccg tgggctgtcg tgcccgacgg cggtcccgag gacgtcgatg cggcggtcgc
      721 cgccgcgcgg gcggccttcg agggggagtg gggtgcgcag accgggttcg cgcgggccgc
      781 cctcctgcgc cgcctgggcg acctcatcgg ggagaacgcc gagcgactgg cccggctcga
      841 ggtgaacgac tccgggaagc tctaccgcga gatgatcggc cagctcaccg gcatcggtac
      901 atggttccac tactacgcgg ggcttgccga caagatcgag ggcaagcaga tcccggccgg
      961 caacccgaac tacctcgtct acacccggcg cgagcccgtc ggggtggtcg ccgcgatcac
     1021 gccgtggaac tccccgctgc tgctgatgac ctggaagctc gcaccggcgc tggcggccgg
     1081 ctgcacggtc gtcgttaagc cctcggaaca cgcaccggcg tccacactcg cgttcgccaa
     1141 gctcgtcgag caggccgggt tcccgcccgg ggtggtcaac gtcgtcaccg ggctctcccg
     1201 cgaggtgggc gccgcgctcg ccgggcaccc cggggtggac aaggtggcgt tcaccgggtc
     1261 cacggccacc ggccgggctg tcgcggctgc agcggccgag aacatcaaca aggtcaccct
     1321 cgagctcggc ggcaagtcgc cgcagatcgt cttcccggac gccgacctgc aggccgcggc
     1381 caatgggctg gtcgcgggcg tgttcgcggc caccggtcag acctgcatgg ccgggtcccg
     1441 gctgatcgtg cacgccgacg tccacgacga gctggtgcgg ctcgtcgccg agcgtgccgc
     1501 gcggatccgg ctgggcgacc cgaacgaccc cggcaccgag atgggcccgg tggcgaaccg
     1561 accgcagtac gagaaggtgc tcggctacct ggcgacggcc cgggccgaag gcgccacgat
     1621 cgcctacggg ggcgcggccg acgaggggct gggcgggctg ttcgtccggc cgacggtgct
     1681 cacgggcgtc accgccgact ccaccgtggt gcgcgaggag gtgttcggcc cggtgctcgc
     1741 cgcgctcacc ttcaccgacg aggacgaggc gctgaagctg gccaacgaca ccccgtacgg
     1801 gctcgccggc gcggtctgga ccaaggacgt gcaccgcgcc catcgggtgg ccgcgcggct
     1861 gcgcgcgggc accgtgtgga tcaacgccta ccgcgtggtg tcgcccggcg tgcccttcgg
     1921 cgggtacggc gccagcggcc tcggccgcga gaacggtgcg gcaggcctcg acgagtacct
     1981 cgagaacaag tcggtgtggg tcgagctcac cggcggcacc cgcgacccgt tcacgctggg
     2041 ctgaccaccc gcctcgggag gaaccggacc tctgcgtcgg ctgcggtcat gaccgagtag
     2101 gtcgagccct cctggccggc ggcaccgccg tggcgcccgt cgggagtgag ggcgagcgtg
     2161 cgcagctcgg cacggaaggt gtcgggcagc gtcgccgcgt cggccagcat ggcggcacag
     2221 gccgactcgg gatcgtcccc ggcggcgagg cggtcgacga cgacccgggc gccggtggcg
     2281 cggatgctca gctcgccacg cccggtgcag gccgccccgc cgtagcgcag gtcgcagtag
     2341 ttgccggcac cggcgatggc cgagtcgccg acgcggccgg ggtacttcca cgggtagccg
     2401 ctggtggaga ccccgaccac caactcgccg ctcccgtcga gggcgaggac gttgatcgtg
     2461 ccccacggtc catcgtgcgg ggccatccgc ttcaccagct ccagcgccgc gagccggtag
     2521 gccacatcgc ccgagccggc ccgggtgttt tcgccctcca ccgactcgtc ggtcaccgac
     2581 aaggcgtcgt gccattccct gcgcgcctca tcggtgagga gctcggcagt gctgaacccg
     2641 cactcggcgg cgaacagctc cgcgccctcg ccgaccagca acgcgtgctg gggaaggtgt
     2701 tccatgacgg cccgggccac cgagatcggg tgcgggtagc cgcgcaccgc ggccacggcc
     2761 ccgaaggcgc gggtggaccc gatcatcacc gaggcgtcca gctccacctc cccgcgggcg
     2821 ttgggcaggc cgccggttcc gacgtagtga tcggacaggt tgtcctcgca cagccgcagc
     2881 gccgcctcca ccgcgtcgag ggcggacccg ccgtcccgca gcacggccat cccggcgggc
     2941 agacctgcct cgctgcgttc gctaccgatg atcaccggtt cggtcaccgg gcacgtcctt
     3001 tcgcggtgag ctgggtcgag gagggtcgtt cggcggcatg ccagcaagcg gcggaccgta
     3061 ccgggtcgtc cggcagcggg agcaggtccg gttccgcggc gcaggcggcg atcgccaccg
     3121 ggcaccgggg ccggaagcgg catccggtcc cctcgccctc gatggtgtga gcggcgaacg
     3181 gcgactcgtc cgcctcgggt tcgccgtcca ctaccgccaa cgggtcggcg gtgccctccg
     3241 cacgggcgcc gtgcacccgc gtcagcatcc gcggaaccga gctcagcagc agctgggtgt
     3301 aggggtgtac cgggtcggtg aacagctcgt cggtcgcgcc cgtctccacg atccggcccc
     3361 ggtacatgac ggcgatccgg tcgctgacct gacgtactac ggccatattg tgcgacacga
     3421 agatcatcga caggccgagc gactccttga ggtcgagcag caggttgatg atcgtcgctt
     3481 ggaccgagac gtccagggcc gagaccggtt cgtccgcgat cagcaccttc ggctcgaggg
     3541 ccagcgcgcg ggcgatggcg atccgctggc gctgcccgcc gctgaactgg cgcggggtgc
     3601 gtcccagcgc gtcgtgcgac agcccgacga ggtccatcag ctcgcggctg cgggccgtga
     3661 cctggtcgcg ggggacgatc tggtggtagc gcagcagctc gccgatggtc tggcccacgg
     3721 tcatccgcgg gttcagcgag ctgtagggat cctggaacac catctgcgtg gtccgccgct
     3781 gcacccggtt cggccggccc tcgaccacgc cgccgccgta gcggatctcc ccggcggtgg
     3841 cctggtacag gcccatgacg gcacggctca acgtcgactt gccgcagccg gactcgccca
     3901 ccaggccgag cgtctcaccc gcggcgagcg tgaggtcgac accgtcgacc gccttgacca
     3961 catgggccgg gcggcgggcc agccggtcgg tccaggtgtg ggcgccgacg aagtgctgaa
     4021 ccagcccgga gacggtcagg acggcatcgc cccggctgtt cgacacagag ggtgtcggtg
     4081 tggtgacggt catgaccggc tctccagcgt atcggcgggc cctggggcgc cgtcccactc
     4141 cggggcggtc tcccagcgga ggcaggcgct gttgtggcgg cccccgctga cggcgacgag
     4201 cgccggctcg gccgcctcgg ccgtgcactg ctcctgggcg aacgggcagc gcggcgcgaa
     4261 cgagcagccg cggtgcagct cggtgaggtc cggcggttgg cccgcgatcg tcagcagccg
     4321 gtgcaccggc atgtccgggt cgggcaccga tcgcagcagc gcaaacgtgt aggggtgccg
     4381 cgggcccgtc agcagctccc tggcctcacc cgactccacg acgcgtccgg catacatgac
     4441 gtagacccgc gagcaggtct ggctgaccac ggccaggtcg tgactcacca ggaccaggcc
     4501 cagctgctcg tcccgacgca gagtgtcgat gaggtcgagc acctgcgcct ggatcgtcac
     4561 gtccagcgcc gtggtcggct cgtcggcgag gatcagccgc ggcgccgccg agagcgcgat
     4621 ggcgatgcag atgcgctgcc gcatgccacc cgacagctcg tgcgggtacg cccggaaccg
     4681 cttttccggg tcgggtatcc cgacgcggtc catcaggtcg acagcccggc tgtgtgcctc
     4741 ggagcgcgac atcccgaacc ggcgccgtgg cacctcggcg atctggtcgc ccacccgcat
     4801 caccgggttc aacgccgtga gcgagtcctg gaagaccatg ctcatcgcct cggtcagcac
     4861 ctgctgcctc cgccgtgcgg gcaggcccgt gacgtcgacg ccatcgagca ggatcgaccc
     4921 tccggtgagg cgcgccgggt gcggcagcag gccgagcaga gcgcgaaggg tcaggctctt
     4981 gcccgagccg gactcgccca ccaccccgat cgcctcgccg gcgtcgacct gcagggacaa
     5041 cccgcggacg gcgtgcagcc gcccgtgcgg cagcgggatc tccacctcca ggtcgtggac
     5101 ctcgagcagc gcatgcgccc cgcccgccac cggtcgggcc gccagttgct ccgactccct
     5161 gcgcacgccg atcatcgctg accgaaccgt tcgacgaggc cgtcggcgac cagcgcgagc
     5221 gcgaggccca cgagcaccac ggcgaggccg gggatcgtgg cgatgtacca ctgggtgagc
     5281 aggaactgct gcccgtcctg gatcatcgag ccccagtccg gcgtcggcgg cggcaccccg
     5341 aggccgaggt agctcaaggt cacgatggcc aggatcgtga gcacgatgtc gctcatcgag
     5401 tagacgacgg cctgcaccac cacgttcggc aggaggtggc ggaacaggat ccgccaggtc
     5461 ggcaggccac tggcctgcgc cgccagcgcg tactcctgct ccttggcccg cagcacctca
     5521 ccgcgcacga tccggcagta cgcgacccag ccgaccatgg agatcgccac atagatgctg
     5581 ccggtgcccg cgccgagcgc gaacaccagc gcgatgacca gcacgaagaa cgggaaggcg
     5641 accacgacgt cgacaacccg catcacgacg agatcgaacc agccgccgat ccagccggtg
     5701 agcaggccca gcacggtgcc cgcgacgaac ggggtgacca cggcgagcac accgacctgc
     5761 aggtcggtcc ggccgccgta gagcagccgg gacagcacgt cgcggccgag ctggtcggtg
     5821 cccagtaggt ggcccggtga ccccggtggg agccgggctt gcaggagatc ctgggcgatc
     5881 gggtccgcgc tcgtcagcag gggcgcacag atcgccgcca gcacgatgag caggaacagg
     5941 gtgagcccgg cgatgagcgt gacgttgacg gagccccgtc gccgtcgcgg agtggccgcg
     6001 tcggtc
//
LOCUS       DASRSC010000072         9388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1170389,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000072 DASRSC010000000
VERSION     DASRSC010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9388
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170389"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..374
                     /locus_tag="VFE39_03560"
     CDS             <1..374
                     /locus_tag="VFE39_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6257708.1"
                     /translation="DAFCGVVSEDAQSAAAGNTITAVELKEMIDAGKDFALIDVREQN
                     EYEIVSIPGARLIPKDRILSGEALSEIPQDKPVVLHCKSGARSAEALSALHKAGFKDA
                     VHVGGGVLAWVKQVDPSLPTY"
     gene            complement(395..556)
                     /locus_tag="VFE39_03565"
                     /pseudo
     CDS             complement(395..556)
                     /locus_tag="VFE39_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640442.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(1430..2050)
                     /locus_tag="VFE39_03570"
     CDS             complement(1430..2050)
                     /locus_tag="VFE39_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257709.1"
                     /translation="MAELDDLASLRSAAIAAGGEPATAKRHLVARPTGIRWTTGGGVL
                     PFPLLPLRARQHLGPARGGAAVSFTVGAVRADDIEHVVTHRDGLLTVVRQGSADRAPH
                     SLLQVLQLRAFRGPLAQAKIPLLVEGGDHQGLVRGQLRLGRGVPQHLLDGLEVLLGEL
                     QIVLRAVQGPVGVDLGLERGLAPFEQRLTVLQRLDRGRWHRRLAIL"
     gene            complement(2569..3441)
                     /locus_tag="VFE39_03575"
     CDS             complement(2569..3441)
                     /locus_tag="VFE39_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257710.1"
                     /translation="MPDEPPDESLDELEPPFDEVFRLVPESSPLLRRELPSESPLDAR
                     CRRSDDFCSDLSAPESRSPDPSSDERWPDDLCPDRPDLSSPESCAPDDLSSDDRCPDR
                     PDDASPESRPPDDASPDDLCPDRPDLSSPESRSPDDLCPDRPDDFPSDRDSSDRDSWA
                     FNRWDFDCGADFVRCVSWPEAADRSPSREPEPVRSDLSDRSERLPESPAASRRAEAMS
                     RTAPCPPTALITARARRTFEADDASPPLSSSDASSPELRKVEDSLPVRLPPEASPAFR
                     SPPEGFALSRSPVL"
     gene            3562..4392
                     /locus_tag="VFE39_03580"
     CDS             3562..4392
                     /locus_tag="VFE39_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02569 family protein"
                     /protein_id="HET6257711.1"
                     /translation="MTAVPVRQQAASAGTALPPHVRTAFGVGDVEPRPVVWAGRRAWH
                     CGDVLVRPVPDNAVAAWSARVLDGFEVEGLRLARPVRSSDGRWVVAGWTACRFLDGTI
                     EPRYDAVVEVATRLHAATAALPRPRLLDDRDDLLTRSASAAFGERRLVLDPAKGGELY
                     ATLATHRTPVRSEPQVVHPELFGAVLFDADGMPALIEMVPCWRPKEWAAAVVVVDALA
                     WGGADDGLLQRWAHLPEWPQMMLRAVLHRLALHAQHPEGSTRTLAGLERVARLVAVLL
                     "
     gene            complement(4403..5296)
                     /locus_tag="VFE39_03585"
     CDS             complement(4403..5296)
                     /locus_tag="VFE39_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HET6257712.1"
                     /translation="MSGAGALTHAEALQQIADRNGGNRATPGPGYDASVDYVVGVLRD
                     AGFDVSTPTFVLRQRGGGRETVVRNVIAQTRTGSAEHVVMAGAHLDSVPEGPGINDNA
                     SGVATLLEVATRMGSSPPIPNVVRFGFWGAEERNLNGSKSYVESLSASEREAIALYLN
                     LDMVASPNAGYFVQGGADGPESRSGPPGSATVGRVLVDELAANGIAADLMRFEGDSDY
                     APFVDADIPSGGVLAGDSNKKTTEQADRWGGRVGDVFDACYHSACDRIETLDRTALDK
                     FSDAIAGTIARFADAAEVPAR"
     gene            5652..6289
                     /locus_tag="VFE39_03590"
                     /pseudo
     CDS             5652..6289
                     /locus_tag="VFE39_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662417.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            6419..6727
                     /gene="nirD"
                     /locus_tag="VFE39_03595"
     CDS             6419..6727
                     /gene="nirD"
                     /locus_tag="VFE39_03595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HET6257713.1"
                     /translation="MCRDDRLLPERGGAALLGDLQVALFRTHDGTVHAIGKIDPFSGA
                     AVLSRGIVGDRGGVATVASPVYKQVFDLVTGRCLDDPAVAVLVFPIRAIDGSLQVGLP
                     "
     gene            6745..7926
                     /locus_tag="VFE39_03600"
     CDS             6745..7926
                     /locus_tag="VFE39_03600"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HET6257714.1"
                     /translation="MTSPSEVPPLAGFTVGVTAARRAAELASMLERRGAVVQHGAALR
                     IVPVADDTQLADATRGLIARPPDITVSTTGIGFRGWVEAADGWGIGEELLAALGAGKL
                     IARGPKARGAIRASGLVDAWSPPSESSAEVLDYLLDGACAGRGVDGLRIAVQLHGEPL
                     PDMVEALTLAGAEVIEVPVYRWMPPVDIGPLDRLIDGVLARCVDVMAFTSAPAAASVL
                     ARAGERGIREELLAALRGPVLSLCVGPVTAAPLEAVDVLTIQPQRSRLGAMVRRLEAE
                     MPGRARPLPVAGHWLELRGHAVLVDGTLRMVPPAGMALLRALARRPGRVVARAELLRS
                     LPGGSGDEHAVETAMARLRTALGEPKLVQTVVKRGYRLALDPAADPVPAGGHCLHGPA
                     R"
     gene            complement(7994..8281)
                     /locus_tag="VFE39_03605"
     CDS             complement(7994..8281)
                     /locus_tag="VFE39_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257715.1"
                     /translation="MQVIDISQEDAEAASLLFSRHLLPSEEFGLSLIAQRHGDEIWFT
                     TSLAWFWVSARGVPRLLPLDGNRGERTEWHEGDEQDRTVGTIRVQVDSLSV"
BASE COUNT         1318 a         3165 c         3511 g         1394 t
ORIGIN      
        1 atgacgcgtt ctgcggtgtg gtgtccgagg acgcccagtc ggccgcggcg gggaacacga
       61 tcacggcggt cgagctcaag gagatgatcg acgcgggcaa ggacttcgcg ctgatcgacg
      121 tccgtgagca gaacgagtac gagatcgtca gtatcccggg cgcgcggctc atccccaagg
      181 accgcatcct ctccggcgag gccctgtcgg agatccccca ggacaagccg gtggtgctgc
      241 actgcaagtc gggcgcccgg tccgccgagg cgctgtcggc gctgcataag gcaggcttca
      301 aggacgcggt gcacgtcggc ggcggggtgc tggcctgggt gaagcaggtc gacccttcgc
      361 tcccgacgta ctgacacacg ccggcggtcc aggctcagcc tgctgggcgc tgcgcggagc
      421 cgatgcgggg gaacgggcgg gtgaataaca ccacctcgac cgccacctcg aagaactcgg
      481 cgatgcgcag cgccagctgc aggctcgggc tgtactcgcc gcgctccagg tatccgaccg
      541 ttctcacgag atgaacatcg cgggcagcag aactatcagg atcgacgcga cggtgtcgag
      601 tgccgcggcc accgacagcc ccaccacgta cgcggtcatc ccgggcggaa ccgccgtgga
      661 gtgacctgac caggggtgat cgctccggat ggagcagtga caaaagatcg ttttgcccgt
      721 agtgtcccga tctccgaaga tcaagacgga cgccgcggcg ttggggtgcg cgtgcgcagc
      781 cacgggggcc tgaccgggcg gcgccgcgtc ctggcgcgtc ctccgtcacc cggcgatggg
      841 ggtcgcactg gtggacgacg cgagacactg cgccctgccc gggtgtggag agttgatcga
      901 ggacatcccc gggcggcctg cccggctcta ctgctcggcg gcacaccgct gcgccgttcg
      961 gcgggcgcgc cgtgccgccg cgctgcgacc cgatcacgac gcgcgtctcg cggagaccct
     1021 gccgtggctg agggagccgg tcgacgatga gcctcccgcg tgcgcgcccg tccggtccgt
     1081 cgaacctctc gagtcagccc gtgcaagcaa cgcgcccgcg gacgggtcgc gctcgcgcgg
     1141 cgggatcgcc gcccgcatgc tggccgggct ggcacgcggc gccggtccgg acgagtcggc
     1201 ggcgcggcga cggcgggccg tggcagtgct cggcgtcgcg ggcatccttg ccggtgggta
     1261 cgcggtcacg gcgtcggagc cgatcagcgg ccccgccgca ctgccggccc agcccgttcc
     1321 ggggccggag acggaggacg agtgggtggc tcgcgcgcag gtgacactga tctcggtgaa
     1381 ccggcagctc gacaccattg cgcagaccga ggaggcctgg agccggctgt cacaggatcg
     1441 ccaggcggcg gtgccacctg ccccggtcaa ggcgctgaag gaccgtaagg cgttgctcga
     1501 acggcgcaag gccacgctcc agtcccagat cgacacctac cggtccctgg accgcacgaa
     1561 ggacgatctg aagctcaccg agcagcacct ccaggccgtc gagaaggtgc tgcggaacgc
     1621 cccgacccag ccgcagctgc ccccggacca ggccttggtg atcgccgccc tcgacgagca
     1681 acgggatctt cgcctgcgcc agcgggccgc gaaacgcgcg gagctggaga acctggagaa
     1741 gggagtgagg agcgcggtcc gcactccctt gccggacgac ggtcaggaga ccgtcgcggt
     1801 gagtaaccac gtgctcgatg tcatccgcgc ggaccgctcc gacggtgaac gagacagcgg
     1861 cgccgccccg cgccggcccg aggtgctggc gcgcgcggag cggcagcagc gggaacggca
     1921 ggacaccgcc accggtggtc caccggatcc ccgtgggccg ggcgacaaga tggcgcttcg
     1981 ccgtggcggg ctcgccgccg gcggcgatcg cggccgaccg gagcgaggcc aggtcatcca
     2041 gctcggccat gccggggacg tgccgacggg cggcggcgcg cggcgggacg acagtccggc
     2101 cgccggaggg aacggtggtt cccgggctgg tggtggatcc ggggtggtcg gtcaggtcgt
     2161 cagtgccgtc ggtggtgtgg tgggaggcgg cggcagtcgt gatggtgagg gtgctggtcg
     2221 ggacgccggg aatggcgggg gcggcactga ccgtgctggt ggcgctcgtg gcggtgaccc
     2281 ctcgtccggc ggtgggaacg gtcgttcgcg ggacgccggt ggcggctctg gcgtggttgg
     2341 tgaggtcacc ggtgccgtcg gcgttgtggc gggtggcggg ggcagcggcg gtcgcggtgg
     2401 tgagagcggt ggtcgctccg gcgcgagccg ggacgcgggt gaccaggacg gtggcggcgg
     2461 tcgtgacggg gacagcggcc ggtccggcgg tgatcggtcc ggcgatgacc ggaccagccg
     2521 acagcggtcc ggcgagaacc ggaacggcga cgaccggtcc gtccgggatc agagcaccgg
     2581 ggaccgcgac agcgcgaacc cctccggtgg ggagcggaac gccggcgagg cctcgggagg
     2641 cagccggaca gggagcgagt cctccacctt gcgcagctcc ggcgacgacg cgtccgatga
     2701 tgacagcggc ggtgaggcgt cgtccgcctc gaaggttcgc ctggcccggg ctgtgatcaa
     2761 cgcggtcggc gggcagggcg cggtgcggga catcgcctcg gccctgcgcg aggcggcggg
     2821 ggactccggc agtcgctccg accggtcaga caggtcggag cggacgggct ccggctcgcg
     2881 cgacggcgac cggtcggcgg cctccggcca ggacacacag cgcacgaagt cggctccgca
     2941 gtcgaagtcc cagcggttga aggcccagga gtcgcggtcc gaggagtcgc ggtccgacgg
     3001 gaagtcgtcc gggcggtccg ggcagaggtc gtcgggcgag cgggactcag gtgaggagag
     3061 gtccgggcgg tctgggcaga ggtcgtcggg tgaggcgtcg tcgggtgggc gggactcggg
     3121 tgaggcgtcg tccgggcggt ccgggcagcg gtcgtccgag gagaggtcat cgggcgcgca
     3181 ggattcgggt gaggagaggt ccgggcggtc tgggcagagg tcgtcgggcc agcgttcgtc
     3241 ggatgagggg tcgggcgagc gggactctgg tgcggagagg tccgagcaga agtcgtcgga
     3301 gcgccggcaa cgcgcatcga gcggcgactc ggacggcagt tcccgccgga gcagcgggga
     3361 cgactccggc acgagccgga aaacctcatc gaagggcggc tccagctcat ccaaggattc
     3421 gtcgggcggc tcgtcgggca ccgggagccg atcgaaatcc tcggagtccg acgactgacg
     3481 ccgatcatcg ctcgcacacc gtctggatct actccgcgtg gcggtcccta tccccgacac
     3541 ggcgcaggta gcgtcccggc cgtgacagct gtccccgtcc gccagcaggc cgcatcggca
     3601 gggaccgccc tgccaccgca cgtccgtacc gcgttcgggg tgggggatgt cgagccgcgg
     3661 ccggtggtct gggcgggtcg gcgtgcgtgg cattgcggag acgtgctggt caggccggtg
     3721 ccggacaacg cggtcgccgc gtggtcggcg cgtgtgctcg acgggttcga ggtggagggg
     3781 ctgcgcctcg cgcggccggt ccgctcctcg gacggccggt gggtcgtggc cggctggacc
     3841 gcctgccggt ttctggacgg caccatcgag ccgcgctatg acgcagtggt cgaggtcgcc
     3901 acgcggctgc acgcagccac ggcggcgctg ccccgcccgc gactgctcga cgaccgcgac
     3961 gatctgctga cgcgctccgc gtccgccgcg ttcggcgagc gcaggctggt gctggacccc
     4021 gcgaagggcg gggagctgta cgcgacgctg gccacgcacc gcacgcccgt ccggagcgag
     4081 ccgcaggtgg tgcaccccga gctgttcggc gccgtcctgt tcgacgccga cggcatgccc
     4141 gcgctgatcg agatggtgcc gtgctggcga ccgaaggaat gggccgccgc ggtcgtggtg
     4201 gtcgacgccc tggcctgggg cggcgccgac gacggcctac tgcagaggtg ggctcacctg
     4261 ccggaatggc cgcagatgat gttgcgggcc gtgctgcatc ggcttgcgct gcacgcccag
     4321 caccctgagg ggtcgacacg cacgctggcc ggcctcgagc gggtggcgcg gctggtcgcc
     4381 gtcctgctct gagaaggccg gctcaccgtg cgggtacctc tgcagcatcg gcgaaacgag
     4441 cgatcgtgcc cgcgatcgcg tcgctgaact tgtccagtgc cgtccggtcg agggtttcga
     4501 tgcggtcgca ggccgagtgg tagcaggcgt cgaagacatc tccgacgcgc ccgccccagc
     4561 gatcggcctg ctcggtcgtc ttcttgttcg agtcacccgc caggacaccg ccggacggga
     4621 tgtcggcgtc gacgaacggc gcgtagtccg agtctccctc gaacctcatg agatcggccg
     4681 cgatgccgtt ggccgccagc tcgtccacga gcacccgtcc cactgtggcg gagccgggcg
     4741 ggccggaccg ggattccggg ccgtccgcgc cgccctggac gaagtagccg gcattcggtg
     4801 aggcgaccat gtccaggttg aggtagagcg cgatggcctc tcgttcgctg gccgagagcg
     4861 actcgacgta gcttttggag ccgttgaggt tgcgctcctc cgctccccag aagccgaagc
     4921 gcaccacgtt ggggatcggc ggggagctgc ccatccgcgt cgcgacctcc aggagtgtgg
     4981 ccacgccgga ggcgttgtcg ttgattcccg ggccctcggg cactgagtcg aggtgcgcac
     5041 cggccatgac cacgtgctcg gcggagccgg tccgggtctg cgcgatcacg ttgcggacga
     5101 cggtctcccg gccacccccg cgctgccgca gcacgaaggt cggcgtgctc acgtcgaagc
     5161 ccgcatcgcg gaggacgccg accacgtagt cgacgctcgc gtcgtagccc ggcccgggag
     5221 tggcccggtt gccgccatta cggtccgcga tctgctgcag ggcctcggcg tgggtcagtg
     5281 ctcccgctcc gctcacctgc tgtaccagct gggaatgcag ggggctccgg gcggcgggtt
     5341 cgggagatgt cggaccggtc gacgtgcagg cgacgagaac ggctacagca gcggcgacgg
     5401 cgccacccca accggcgtac cgacgccagg cgtccgacga gcgcggtggc actcgttctt
     5461 ccctggtgat cgctccacgc acgatgcctc acatctgcgc ccacggtcgg tgagcgccgc
     5521 tttgcgtcca agtcacacgg aaccgactga gcggtgacaa gcgcttccta gcgtcgccca
     5581 cgagggcagc gccgcccaca gctcccacca ccagacacaa cgaccgccga accgcggcga
     5641 cccaacagct catccagagg cccgacgcat attctgcccg atcccggctc accgggcatt
     5701 ggatcgatca ttgggacccg gagaatcagc cttctgggta cacaccggtg ccaaaatcgc
     5761 caaccgcaac ctgtggttct cgatcctcag cgagcacatc ggatggtcga tctggtcgat
     5821 ctggtccggt actcgtgctg ttcatgggcc cggagtacgg gatcgagcag cggggaagtt
     5881 catcctggtc ggcgtgccca cgctggtggg ctcggtcctg aggctgccgt actcgttcgc
     5941 cgtggcccgc ttcggcggcc ggaactggac gatcatcagc gcggcgctcc aactgatccc
     6001 caccattttc gccgcgatca tcatgcagct gggaatatcc ttcacgactt tcctcgtgac
     6061 ggcggccgtc gcgggggccg acgggggcaa cttcgcctcg tccatgacca atatcaacac
     6121 tttcttccct gagtcgcgta agggatgggc gctcggttcg aacgcgggcg gcggcaacct
     6181 cggcgtgccg gtcatccagc ccgtcggtct gctgatcatc accgcgggcg ccggcgcgcc
     6241 gcgaatcctg ctcggcgtct acatcccgct gatcgtggtg gcagccgtgg tccgaaggcg
     6301 ctgctcgtgt tcgggtccaa cgtgctggtc tccgcgcccg acgcgaacgc gctcgacccg
     6361 atcagccgga tgccggagtt ccgctggcag accgtgtgcc gcgacgaccg ggcagaccgt
     6421 gtgccgcgac gaccggctac tgcccgagcg cggtggcgcg gccctgctgg gcgacctgca
     6481 ggttgccctg ttccgcaccc acgacgggac cgtccacgcg atcggcaaga tcgacccgtt
     6541 ctccggcgcc gcggtgctgt cgcggggcat cgtcggcgac cgcgggggcg tggccaccgt
     6601 cgcatcgccg gtgtacaaac aggtgttcga cctggtcact ggccgctgcc tggatgaccc
     6661 cgccgtcgcg gtgcttgtct ttcccatccg ggcgatcgac gggtcgctgc aggtcgggct
     6721 accgtgaaca gtcctgtgac aatcgtgact tccccttccg aggtcccgcc gctggccggc
     6781 ttcaccgtcg gcgtcactgc cgcgcgccga gccgccgagc tcgccagcat gctggaacgg
     6841 cgcggagcgg tcgtgcagca cggcgccgcg ctgcggatcg tcccggtcgc cgacgacacg
     6901 cagctcgccg acgccacccg cggcctcatc gcgcgtccgc ccgacatcac ggtgtccacc
     6961 accggcatcg gcttccgcgg ctgggtggag gccgccgacg gctggggaat cggcgaggag
     7021 ctgctcgccg cactcggcgc cgggaagctg atcgcccgcg gcccgaaggc gcgcggggcg
     7081 attcgggcgt ccgggctggt cgacgcgtgg tcgccgccgt cggagtcctc ggccgaggtg
     7141 ctggactacc tgctcgacgg cgcctgcgcc ggccgcggag tggacggtct gcgcatcgcc
     7201 gtccagctgc acggcgagcc actgcccgac atggtcgagg cgctcacgct cgccggtgcc
     7261 gaggtgatcg aggtgccggt gtaccgctgg atgccgcccg tcgacatcgg cccgctcgac
     7321 cggctgatcg acggggtgct cgcccgctgc gtcgacgtca tggcgttcac cagtgccccc
     7381 gccgcggcca gcgtgctggc cagggcgggt gagcgcggga tccgtgagga gctgctcgcc
     7441 gcgctgcggg gcccggtgct gtcgctgtgc gtcggtcccg tgaccgcggc gccgctggag
     7501 gccgtcgacg tgctgacgat ccagccccag cgctcccggc tcggggcgat ggtgcggcgc
     7561 ctggaggccg agatgccggg gcgggcgcgg ccgctgccgg tggccgggca ctggctcgag
     7621 ctgcgcgggc acgccgtgct cgtcgacggg acgctgcgca tggtgccgcc cgcggggatg
     7681 gcgctgctgc gggccctggc ccgtcggccg ggcagggtcg tggcacgcgc ggagctgctg
     7741 cggtcgctac ccggtggaag cggcgacgag cacgctgtcg agacggcgat ggcgcggctg
     7801 cgcaccgcgc tgggcgagcc gaagctcgtg cagaccgtcg tcaagcgtgg ctaccggctc
     7861 gcgctggatc ccgccgcaga cccggttcct gccgggggtc actgcctgca cgggccagcc
     7921 cggtaaacct gccccgcccg ccccgcccgc gacatggaca catgtcggga gagcacccgc
     7981 ctgacgtgct gatttacacc gacaggctat ctacctgcac gcggatcgtg cccacggtgc
     8041 ggtcttgctc gtcgccctcg tgccactcgg ttcgctcgcc gcggttgccg tcgagcggca
     8101 gcaaccgcgg tacgccccgg gcgctgaccc agaaccaggc gagggaggtc gtgaaccaga
     8161 tttcgtctcc gtggcgctgg gcaatgagag ataggccgaa ctcctcgctg ggcagcaggt
     8221 gtcggctgaa cagcagcgac gcggcctcgg cgtcctcctg cgaaatgtcg atcacctgca
     8281 cggttccgcg gcggtccagt tggctatcgc gcgagtcgag cagggactgg tggccccaac
     8341 cgggatcttg acgatccact gagacgggcg gccgtctggt gcggtggtgc tcacccccag
     8401 gaccgctgcc tgtcccagtc cgggcgacag gtccagcacc gagagcactc gcgttccggc
     8461 cccccatgcg ccctcatcgg atgcgagtac ggcctcgatg gtctcagcat cgggcaggcc
     8521 aaggtcggtc atctgtttcc ttgagggcgg cggtccacga gtggggtctc ggacgaagag
     8581 gaccaccggt gcgacgggaa ctcatgccga tttcggcatg aacccggtgg tgtacttcga
     8641 ggacgctggt gctcagggtg ccgggttgtc tcgcaccctg gaggtggagc accggtggct
     8701 ggctgggctg cgaactgcgg ccgatgggcc gacgccgtga ccacattggg cggtggccgc
     8761 gctcacaggt cgtgtttcgg cgtggtgctg cgtgggtagt cgtggcgcga tgtctccttc
     8821 agccgcgagg gatgaggtcg ccgagcgggc ggcccgcgat gtggcgcgcc tcctggtagg
     8881 catggtgggg ctgcggcgcg cggtcgagtt ggccgcggag gcgaacgcgg agtggacggc
     8941 ggagctgagc gctgaggcgc caagccgtac ctctgcggtg gcgtagctgt ctggcggcgc
     9001 tctgccgacg cggtcaggga ggccgaccgc gcggcagttt ttctctggtc gattctccca
     9061 aaacttgtca tagatgttcc ccacagtttc cccattccgt ttccgcctgt ctcgttcacg
     9121 ccgcgcattc cgaaaacatg attgccggac aggcgggcgc ggcggaggct gctgccgggt
     9181 gcaggggcga aagaagaaga acaagaacga ccagaagacc ggagcggtgg aagacgtgct
     9241 gcgccctcga catctgaacc tcctaggtct tcagacgtac gagggcagcc ctgaccctgg
     9301 ctgctcatcg tcgcatggcc ccagccggag caacctactc cgccttcaaa gcatgttctt
     9361 gctgccggca gcaccctacg acagcgct
//
LOCUS       DASRSC010000073        10918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3789715,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000073 DASRSC010000000
VERSION     DASRSC010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3789715"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..1069)
                     /locus_tag="VFE39_03610"
     CDS             complement(257..1069)
                     /locus_tag="VFE39_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HET6257716.1"
                     /translation="MANGFTKAWKYLMALFSSKVDEYADPKVQIQQAIEDAQRQHQAL
                     SQQAAAVIGNQRQLEMKLNRQLGEIEKLQASARQALVLADQARTAGDEVTAQQREQAA
                     QAFATQLVTAEQSVEDLKTLHDQSIQAASQAKTAVERNSMMLQQKIAERTKLLSQLEQ
                     AKMQEQAAASLRSMTELAAPGNTPSLEEVRDKIERRYANAIGSAELAQNSVQGRMLEV
                     QASTVDMAGASRLEQIRASLHGTPVAGELKAGDTAAAPAQPQLNKRQQPGTA"
     gene            complement(1354..1788)
                     /locus_tag="VFE39_03615"
     CDS             complement(1354..1788)
                     /locus_tag="VFE39_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6257717.1"
                     /translation="MAVLMREAIGGSLRRARTARKRTLRDVSRRARVSLGYLSEVERG
                     RKEPSSELLAAICAALDIALHDLLTEAAEAMAADALAADAVRLRRPIGLRHMDLRIAG
                     ASDDPSMLPMAPVAPVSPRRPVPQPAPQPVALRPADAVCAAA"
     gene            complement(1918..2532)
                     /gene="pgsA"
                     /locus_tag="VFE39_03620"
     CDS             complement(1918..2532)
                     /gene="pgsA"
                     /locus_tag="VFE39_03620"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET6257718.1"
                     /translation="MNPAPAGPPPLLNIANVLTGVRLALVPVFVAVLLTEDGTSTSWR
                     LAAFGVFAVAAITDRYDGELARRRGLVTAFGTIADPIADKALTGSALIGLSALGMVPW
                     WATVVIMVREIGVTVLRFLVLRHGIIPASRGGKAKTLVQTLAIGLYLLPLTELFHAAT
                     GAVELLRWTVLVVAVVLTVVTGLDYVLRAARLRSAAAAALPDTS"
     gene            complement(2529..4061)
                     /gene="rimO"
                     /locus_tag="VFE39_03625"
     CDS             complement(2529..4061)
                     /gene="rimO"
                     /locus_tag="VFE39_03625"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HET6257719.1"
                     /translation="MPEPRSVKARSADAPRRAALLTLGCARNEVDSEELAGRLEGSGW
                     ELVDADGDGAAPDVIVVNTCGFVEQAKKDSIDTLLAASDAAQASGAKVVAVGCLAERY
                     GAELAESLPEADAVLGFDSYPDLAERLSDVIGGHAPAPHVPVDRRTLLPLSPVERPAA
                     ALDVAVPGHAWVPNLARTRLSDGPVAPIKLASGCDRRCAFCAIPSFRGAFVSRPPDDV
                     VAEAAWLGMHGVRELVLVSENSTSYGKDLPGGTRALVQLLPRLANVPGIDRIRLSYLQ
                     PAEVRPDLLRVIAATPGVAPYFDLSFQHASPAVLRRMRRFGSREDFLDLCARIRELAP
                     EAGIRSNVIVGFPGETAADLAELEEFLVGARLDAVGVFGYSDEDGTEAEGYDGKLPAE
                     EVAARVSRIAALVDELMAQRAEDRVGTEVEVLVEAAEDEDFECTGRAAHQAPEVDGEC
                     VFERGSGLAIGDLVRAVVVGTEGTDLVVSPRDLLPRSDLVAAPPRSAGASRVGAAARE
                     SG"
     gene            4105..4614
                     /locus_tag="VFE39_03630"
     CDS             4105..4614
                     /locus_tag="VFE39_03630"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HET6257720.1"
                     /translation="MATVAVRRARTADVTTIKQLVDSYAGRVLLAKEIVTLYESVQEF
                     LVAELDGEVVGCGALHVLWQDLGEIRTVAVYPRVVGRGVGHALVTALIANARELGLRR
                     LFVLTFEKQFFAHHGFAEIDGTPVEPDVFAAMLRSYDAGVAEFLDLAHVKPNTLGNVR
                     MLLQLPAEP"
     gene            4664..5503
                     /locus_tag="VFE39_03635"
     CDS             4664..5503
                     /locus_tag="VFE39_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6257721.1"
                     /translation="MAAPVCVPVPETWQMRERRPAAPLRPYISSYTGYRMEASPRSTH
                     HGVASPHLTFLLCLDGKVEILANADRSKPPGAFVAMVGGLHDGPAEIAQGEPQTGLQL
                     RLTWRGARMLLGVPAGELAGDVVDLDALLGRRSAMLLDRLATAPGWPERFALLDAELA
                     ALAARGRGAAGVAPEVGYTWDRLEETGGNLRIAELAREVGWSRRHLAEKFRAETGLAP
                     KAAARVIRFEGACDRLRGPHRPPLARVAADAGYVDQAHLSRDFRRLAGITATAWLAER
                     RPD"
     gene            complement(5687..6562)
                     /locus_tag="VFE39_03640"
     CDS             complement(5687..6562)
                     /locus_tag="VFE39_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET6257722.1"
                     /translation="MFWPRRRRNGKKQVKGGRQALMPIPLTGGLLSGEVRDNEGKPLA
                     GAEVSIFDRASKRVARSDTDLYGFFAAAVMPGSYRIRAEAGGYQSQYARTEVEWGTHN
                     ALGDLTLVPDERLTVPTPGLYEIDPEHSAIRFVARHIAMSRVYGQFIEFSGYIHIAER
                     FEDSQVEVQIDAASVFTNAEKRDAHLRSADFMDVARYPTLLFASHRFSRPGGNRWLID
                     GELTLHGMTSDVQLDATYLGMQEWNGMRAGCTATTELHREHFTVNWQEILARGVPVVG
                     STIQITLDIQGVLQT"
     gene            complement(6722..9265)
                     /locus_tag="VFE39_03645"
     CDS             complement(6722..9265)
                     /locus_tag="VFE39_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HET6257723.1"
                     /translation="MAGRTTAGGGRGTAAAASRVTGGRKPAARKPAARKPAARSTARR
                     PAARKPAPRRGPDLIDRGIDAVGRGIAGLGRSLGRVVGRARELDPAHRRDGLGVAFLV
                     VAVVSAAGIWWGAGGPVGRALSATVGYVVGLGAAVLPVLLAVVGVALMATQPHPEARP
                     RVAVGSLLLVLGVLGLVHLAAGRPDEPGLWVDGGGALGYLAATPLATGLTAWVAVPVL
                     VLLSGYAFLVLTGTPVREVPHRIRRLLGRAVEEPAAATGDETTADPVTDTPPVALRRP
                     SRRRQASRVADVYREDSDDTTETPDVSAAPEERPARPRRKPPATVPATPPAEPEPEGA
                     VGEQLRLTVSPSEGETTYVLPPSDLLPTGPAPRTRSSANDAMIESITGVLDQFAVDAQ
                     VTGFTRGPTVTRYEIELGPAVKVEKITQLTKNISYAVATDNVRILAPIPGKSAVGIEV
                     PNTDREMVRLGDVLRSGNARAEQHPLVIGLGKDIEGHFLTANLAKMPHLLVAGSTGSG
                     KSSFVNSMLVSLLSRATPDEVRMILIDPKMVELTPYEGIPHLITPIITQPKKAASALA
                     WLVEEMEQRYQDMQVNKVRHVDDFNRKVRSGEITAPLGSEREYRPYPYILCIVDELAD
                     LMMTAPRDVEDAVVRITQKARAAGIHLVLATQRPSVDVVTGLIKTNVPSRLAFATSSL
                     TDSRVILDQPGAEKLIGMGDGLYLPMGAGKPVRMQGAYVGDEEISSVVAFTKDQAEPT
                     YTDGVTVQKPGEAKDVDPEIGDDLDVLLQAAELVVTSQFGSTSMLQRKLRVGFAKAGR
                     LMDLLESRGVVGPSEGSKARDVLVKPDELDSVLWLMRGGGGPDADDPDD"
     gene            9376..9861
                     /locus_tag="VFE39_03650"
     CDS             9376..9861
                     /locus_tag="VFE39_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257724.1"
                     /translation="MTDHGAHAFDFIHGSWRVHNTKLRDTTDPSCEEWVEFDATSEVF
                     PVLRGVGHVDRMDVPDPPDGDPFEGFTLRLFDPAAATWSIWWSSSRNPGHLEPPMVGA
                     FTGDHGVFLADDVVGGHAVTVRFEWRADPASPRWQQSFSYDAGRTWRPNWTMDFSRRA
                     G"
     gene            complement(9889..10311)
                     /locus_tag="VFE39_03655"
     CDS             complement(9889..10311)
                     /locus_tag="VFE39_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257725.1"
                     /translation="MISAATLRRLPVRLTAGAYILNSGLQKWSADEQTAGALHGMASN
                     AYPVLKQVDPPMFLRGLAAAEIGLGAALVLPAVPALLAGAGLTAFSAGLLGLFVRTPG
                     MREEGSLRPSQQGIPFAKDVWLLGIGVGLVVDALTSRE"
     gene            complement(10360..10800)
                     /locus_tag="VFE39_03660"
     CDS             complement(10360..10800)
                     /locus_tag="VFE39_03660"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET6257726.1"
                     /translation="MTPTDRQIDDLDRRLLMLLAADGRAPMAVLGRSIGLSRTAVLAR
                     VQRLERDGVIRGYRADVVLPGDAAAHRARVGIVIRTPDVAGYVRRLGALPGVTEIETV
                     TGEFDLIALVAAPSAGELDAVLDRVSGWRETVRTTTWVVLRRYA"
BASE COUNT         1536 a         3969 c         3881 g         1532 t
ORIGIN      
        1 gacgccccgc caccactgac cgcacccccc gcgagtcgcc cgtttcccgc ccccgagtcg
       61 cccgccgcac acccgcgagt cgcccagttt ccgcccccga gtcgcgctat caacgctccc
      121 gagtcgcccg agctgcctcc cgacgccgcc accggtgtcc atgacgcgaa cgagccgccc
      181 ccgaacgacc gacgccgccc gccccgtctg gggcgggcgg cgtcggaggt ggtgcgagcg
      241 actcagtgcg agcagctcag gcagtgccag gctgctgccg cttgttgagc tgcggctggg
      301 cgggcgcggc ggcggtgtcg cccgccttga gctcacctgc caccggggtg ccgtgcagcg
      361 aggcgcggat ctgctccagg cggctcgccc cggccatgtc cacagtggac gcctgaacct
      421 ccagcatgcg gccctgcacc gagttctgtg cgagctcggc cgagccgatg gcgttggcgt
      481 agcgccgctc gatcttgtcg cggacctcct ccagcgacgg ggtgttgccc ggcgcggcga
      541 gctccgtcat cgatcgcaat gacgccgccg cctgctcctg catcttcgcc tgctcgagct
      601 ggctgagcag cttcgtgcgc tcggcgatct tctgctgcag catcatcgag ttgcgctcga
      661 cggcagtctt cgcctgggag gcggcctgga tcgactggtc gtgcaacgtc ttgaggtcct
      721 cgacggactg ctccgcggtg accagctgcg tggcgaacgc ctgcgcagcc tgctcccgct
      781 gctgtgcggt gacctcgtcg cccgcagtgc gggcctggtc ggccagcacg agcgcctgcc
      841 gggccgacgc ctgcagcttc tcgatctcac cgagctgccg gttgagcttc atctccagct
      901 ggcgctggtt gccgatcacc gcagccgcct gctgtgacag cgcctggtgc tgccgctggg
      961 cgtcctcgat cgcctgctgg atctgcacct tcgggtcggc gtactcgtcg accttggacg
     1021 agaacagcgc catcaagtac ttccaggcct tcgtgaaacc gttggccatc tgctccgccc
     1081 tccgagtgcg gcgccgctcg ccgctgcatg tgttatctgg gccgtccggc gtggccgccg
     1141 ggtgcgccga cctccatcgt ggcatcggac cggcccggct tccaccggtc gccgacgata
     1201 tcggggatca tccggaggcc gccgtcgggg cgacccggag gtcaggctac cgctcacacg
     1261 ctccgtgcgg gtgccggtcg cgtggcgcga acgacaccgg cacccgcccg aagagcgggt
     1321 gccgggttgc gacaggcggt ggttgggccg gtgtcaggcg gcggcgcaga ccgcgtcggc
     1381 gggccgcaac gccactggct ggggtgccgg ctgcgggacg gggcgacgcg gagagaccgg
     1441 tgcgaccggg gccatgggca gcatcgatgg atcgtcggac gcgccggcga tccgcaggtc
     1501 catgtgccgc aggccgatcg gacggcgcag ccgtacggcg tccgccgcca acgcgtcggc
     1561 cgccatggcc tccgcggcct cggtgagcag gtcgtgcagc gcgatgtcga gtgccgcgca
     1621 gatggcggcg agaagctcgc tggagggctc cttccggcca cgctccacct cggacaggta
     1681 cccgaggctc acccgcgcac gccgcgacac atcccggagg gtgcgcttgc gcgctgtacg
     1741 agcccgccgc aggctgccgc cgatcgcctc ccgcatcagc accgccatgc gaccctcctc
     1801 gtcgtccgcc ggctgctgtg gacaccgcgc cggtgagatc accgtaccga ctcctgccct
     1861 gcgacgtcgt cgcgcgcggc cgaggtcccg tccgcccacg gcgaacacgg ctcggggtca
     1921 gctggtgtcg gggagtgcgg cagcggccgc tgagcgcagc cgcgcggccc gtagcacgta
     1981 gtccaacccg gtcacgacgg tgaggacgac cgccaccacg agcacggtcc agcgcagcag
     2041 ctcgacggcg cctgttgcgg cgtggaacag ctctgtgagg gggagcaggt agagcccgat
     2101 cgcgagcgtc tgcaccagtg tcttggcctt gcccccacgg ctggccggga tgatcccgtg
     2161 tcgcagcacc aggaaacgca gcaccgtcac gccgatctcc cggaccatga tcacgacggt
     2221 ggcccaccac ggcaccattc ccagcgccga cagcccgatc agggccgagc cggtgagggc
     2281 cttgtcggcg atcggatcgg cgatcgtgcc gaacgccgtg acgagaccgc gccggcgcgc
     2341 cagctcgccg tcgtaccggt cggtgatcgc cgcgaccgcg aacacgccga aggcggcgag
     2401 acgccagctc gtgctcgtgc cgtcctcggt gaggagcacc gcgacgaaca ccggcaccag
     2461 cgccagccgg acgccggtga gcacgttcgc gatgttcagc agcggcggcg ggccggcggg
     2521 cgcagggttc accccgactc ccgcgcggcg gcacccacgc gcgacgcgcc ggcgctccgc
     2581 ggaggggcag cgaccaggtc gctgcgtggc agcagatcgc gcggcgagac gacgaggtcg
     2641 gtgccctcgg tgccgacgac gaccgcccgc accaggtcgc cgatggccag cccggacccg
     2701 cgctcgaaca cgcactcgcc gtcgacctcg ggtgcctggt gcgctgcccg gccggtgcac
     2761 tcgaagtcct cgtcctcggc cgcctcgacg agcacctcga cctctgtgcc gacgcggtcc
     2821 tccgcacgct gggccatgag ctcgtcgacc agcgcggcga tgcgcgagac ccgtgcggcg
     2881 acctcctcgg ccggcagctt gccgtcgtag ccctccgcct ccgtgccgtc ctcgtccgag
     2941 tagccgaaca caccgaccgc atcgagcctc gcaccgacga ggaactcctc cagctcggcg
     3001 aggtcggcag ccgtctcacc ggggaagccc acgatgacgt tgctacggat cccggcctcc
     3061 ggggcgagct cccggatgcg ggcacatagg tcgaggaagt cctcccgcga gccgaaccgg
     3121 cgcatccgcc gcagcaccgc aggactcgcg tgctggaacg acaggtcgaa gtacggcgcg
     3181 acgcctggcg tcgctgcgat gacccggagc aggtcggggc ggacctccgc gggctgcagg
     3241 tagctgaggc ggatgcggtc gatgcccggc acgttcgcca gccgcggcag gagctgcacc
     3301 agagcgcgcg taccaccggg cagatccttg ccgtaggacg tggagttctc gctgacgagg
     3361 acgagctccc gcaccccgtg catgcccagc cacgcggcct cggccaccac gtcgtccggc
     3421 ggccgcgaga cgaacgcgcc gcggaacgag ggaatggcac agaacgcgca ccggcggtcg
     3481 cagcccgagg ccagcttgat cggcgcgacc ggcccgtcgg acaggcgcgt gcgtgcgagg
     3541 ttcggcaccc aggcgtgccc gggcaccgcg acgtccagcg cggcggccgg gcgctcgacg
     3601 ggcgagagcg gcagcagcgt gcggcgatcg accgggacgt gcggtgcggg cgcgtggccg
     3661 ccgatgacat cggagaggcg ttcggcgagg tcggggtagg agtcgaagcc gagcacggcg
     3721 tcggcctcgg ggaggctctc ggccagctcg gcgccgtagc gctcggccag gcacccgacc
     3781 gcgacgacct tcgcgccgct ggcctgcgcg gcatccgacg cggccaggag ggtgtcgatc
     3841 gagtccttct tcgcctgctc gacgaaaccg caggtgttga cgacgatcac gtccggcgcc
     3901 gccccgtcac cgtcggcgtc gaccagctcc cagccggaac cttccagcct gccggcgagc
     3961 tcctccgagt cgacctcgtt tcgggcgcag cccagggtga gcagggcagc acggcgcgga
     4021 gcgtcagcgg agcgggcctt gacgctgcgg ggttctggca cgggcaccag ggtatccgga
     4081 gccggtgacc tagtctgacc cgtcgtggca acggtggccg tacggcgggc caggaccgcc
     4141 gacgtgacga cgatcaaaca gctcgtggac tcctacgcgg gacgtgtgct gctggccaag
     4201 gagatcgtca cgctgtacga gtccgtgcag gagttcctgg tggcggagct cgacggcgag
     4261 gtggtcggat gcggcgccct gcacgtcttg tggcaggacc tcggggagat ccgcaccgtt
     4321 gcggtgtacc cgcgggtcgt gggccgcggc gtggggcacg cgctcgtcac cgcgctcatc
     4381 gccaacgccc gcgagctggg gctgcggcgg ttgttcgtgc tgacgttcga gaagcagttc
     4441 ttcgcccacc acgggttcgc ggagatcgac ggcaccccgg tcgagccgga cgtcttcgcg
     4501 gcgatgctgc gctcctacga cgccggcgtg gccgagttcc tcgacctcgc gcacgtcaag
     4561 cccaacacgc tgggcaatgt gcggatgctc ctgcagctgc ccgccgagcc ctgagccgat
     4621 cttcccgcgg gccaatgtca caccccgccg gtagcctgcg ctcatggccg caccggtctg
     4681 cgtgcccgtc cccgagacgt ggcagatgcg cgagcgccga cccgccgccc cgctgcgccc
     4741 gtacatcagc tcctacacgg gctaccggat ggaggcctcg ccgcgctcca cgcaccacgg
     4801 ggtggcgtcc ccgcacctca cattcctgct ctgcctcgac gggaaggtcg agatactggc
     4861 caacgccgac cggagcaagc cgcccggcgc tttcgtcgcg atggtcggcg gcctgcacga
     4921 cggccccgcc gagatcgccc agggcgagcc gcagaccggc ctgcagctac ggctcacctg
     4981 gcgcggagca cgcatgctgc tcggcgtgcc tgccggcgag ctcgccggtg acgtcgtcga
     5041 tctcgatgcg ctgctgggcc gtcgcagcgc catgctgctc gaccggctag ccacggcgcc
     5101 cggctggccg gagcgcttcg ctctcctcga cgccgagctg gcggccctcg ccgcacgggg
     5161 ccgcggtgcc gcaggcgtgg cgcccgaggt cggttacacg tgggaccgcc tggaggagac
     5221 gggcggcaac ctgcggatcg cagaactcgc gcgcgaggtc ggatggagcc gccggcatct
     5281 ggcggagaag ttccgtgcgg agaccggcct cgcgccgaag gcagcggccc gggtgatccg
     5341 tttcgaaggg gcctgcgacc ggctgcgcgg cccgcaccgg cccccactcg cccgcgtcgc
     5401 cgccgacgca ggctacgtcg accaggccca cctctcccgc gacttccgcc gtctcgccgg
     5461 catcaccgcc acagcctggc tcgccgaacg ccgccccgac tgacccaccg cctgatcggg
     5521 caactcccct gaaccgcgtg cccagtccag cggacgcaac ggcaggacgg gcgaccggcg
     5581 caccagaacc gggcgactcg cgcaccagaa cgggcgactc gcgcagcaga aacgggcgac
     5641 tcgcgcacca gaaccgggcg actggctggg tgggacgact cccggatcag gtctgcagca
     5701 cgccctgaat gtcgagggtg atctggatcg tcgacccgac caccggcaca ccgcgggcga
     5761 ggatctcctg ccagttgacc gtgaagtgct cgcggtgcag ctcagtagtg gcggtgcagc
     5821 cggcacgcat tccgttccac tcctgcatac cgaggtaggt ggcgtcgagc tgcacgtcgc
     5881 tcgtcatccc gtggagggtg agctcgccgt cgatgagcca gcggttgccc ccagggcggg
     5941 agaagcggtg gctggcgaag agcagcgtcg ggtagcgggc cacgtccatg aagtcggccg
     6001 agcgcaggtg cgcgtcgcgc ttctcggcgt tggtgaatac gcttgcggcg tcgatctgca
     6061 cctcgacctg ggagtcctcg aagcgttcgg cgatgtggat gtacccggag aactcgatga
     6121 actgcccgta gacccgggac atcgcgatgt ggcgggccac gaaccggatc gccgagtgct
     6181 cggggtcgat ctcgtagagc ccgggtgtgg ggacggtcag cctctcgtcc ggcaccagcg
     6241 tgagatcgcc gagcgcattg tgggtgcccc actcgacctc ggttcgggcg tactggctct
     6301 ggtacccgcc ggcctccgcc cggatccggt agctgccggg catcaccgcg gcggcgaaga
     6361 agccgtagag gtcggtgtcg gagcgcgcca cgcgcttgct ggcccggtcg aagatcgaga
     6421 cctcggctcc cgcgaggggc ttgccctcgt tgtcacgcac ctcgccgctc aacaggccgc
     6481 cggtgagcgg tatcggcatc agggcctgcc gaccgccctt gacctgcttc ttgccgttcc
     6541 gacggcgcct gggccagaac atgccctgcg acctcccccg acgatatgcc cgctcgaccc
     6601 tgatcgaccc cgaaatcgat ccacactctg acacgctgcg tgaccgagcg acgtccgacc
     6661 cctgtgacgc gcaccacgcg aacagggagt cgtgcgcgct ttcgtggtcg gacggtgacc
     6721 gtcagtcgtc ggggtcgtcg gcgtccgggc ctccgccgcc gcgcatcagc cagagcacgc
     6781 tgtccagctc gtcgggcttg accagcacgt cgcgagcctt cgaaccctcg gacggcccga
     6841 cgacaccccg ggactcgagc aggtccatga gccgcccggc cttggcgaag ccgacgcgga
     6901 gcttgcgctg cagcatcgag gtggacccga actgtgaggt gaccaccagc tcggcggcct
     6961 gcagcagcac gtcgaggtcg tcgccgatct cggggtcgac atccttggcc tcgccgggct
     7021 tctggacagt cacgccgtcg gtgtaggtcg gctctgcctg gtccttggtg aacgcgacga
     7081 ctgaggagat ctcctcgtca ccgacgtagg caccctgcat ccggacgggc ttgcccgcgc
     7141 ccatcggcag gtagagcccg tcgcccatgc cgatcagctt ctccgcgccc ggctggtcca
     7201 ggatcacccg ggagtcggtg agcgacgacg tggcgaacgc gagccgggac ggcacgttcg
     7261 tcttgatcag gccggtgacc acgtccaccg acgggcgctg cgtggccagc acgaggtgga
     7321 tgccggccgc ccgcgccttc tgcgtgatac gcacgaccgc gtcctcgacg tcacgcgggg
     7381 cagtcatcat caggtcggcg agctcgtcga cgatgcacaa gatgtagggg tacgggcggt
     7441 actcccgctc actcccgagc ggtgctgtga tctcgccgga ccgcaccttg cggttgaagt
     7501 cgtcgacgtg acggaccttg ttgacctgca tgtcctgata gcgctgctcc atctcctcca
     7561 ccagccaggc gagcgcggac gccgccttct tcggctgggt gatgatgggc gtgatcaggt
     7621 gcgggatgcc ctcgtagggc gtgagctcga ccatcttggg gtcgatgagg atcatccgga
     7681 cctcgtccgg cgtagcgcgc gagagcagcg acacgagcat ggagttgacg aagctcgact
     7741 tgccggaccc ggtggagccg gcgaccagca ggtgcggcat cttcgccagg ttggcggtga
     7801 ggaagtggcc ctcgatgtcc ttgcccagcc cgatcaccag cgggtgctgt tcggctcgcg
     7861 cgtttccgct gcgcagcacg tcgccgaggc ggaccatctc ccggtcggtg ttcggcacct
     7921 cgatgccgac ggccgacttg cccgggatcg gcgccaggat ccggacgttg tcggtggcga
     7981 cggcgtagga gatgttcttg gtcagctggg tgatcttctc gaccttgacg gccgggccga
     8041 gctcgatctc gtagcgggtg acggtggggc cgcgggtgaa gccggtgacc tgagcgtcca
     8101 ccgcgaactg gtcgagcacg ccggtgatcg actcgatcat cgcgtcgttg gcgctgctgc
     8161 gggtcctggg cgccggcccg gtgggcagca ggtcgctcgg cggcaggacg taggtggtct
     8221 ccccctcgct cggcgagacg gtgagccgca gctgctcgcc cacggcgccc tcgggctccg
     8281 gctccgcagg cggcgtagcc ggcaccgtgg cggggggctt gcgcctcggg cgcgcaggcc
     8341 gttcctcggg ggcggcggag acgtccggcg tctccgtggt gtcgtcggag tcctcccggt
     8401 agacgtccgc cacccgggac gcctgccgtc gccgtgacgg ccgcctcagg gccacgggtg
     8461 gcgtgtcggt gacggggtcg gccgtcgtct cgtcgccggt ggcggcggcc ggctcctcga
     8521 cagcccggcc gagcagtcga cggatgcggt ggggcacctc ccgcacgggc gtgcccgtga
     8581 gcaccaggaa cgcgtagccg gacagcagca cgagcacggg caccgcgacc cacgccgtca
     8641 ggccggtggc gagcggtgtg gcggccaggt aacccagcgc gcccccgccg tcgacccaga
     8701 gccctggctc gtcggggcga cccgcggcga ggtgcaccag tccgagcacg ccgagcacca
     8761 ggagcaggga tccgacggcc acccggggcc gcgcctccgg gtgcggttgc gtagccatca
     8821 gcgcgacacc gaccaccgcc agcaacaccg ggagcaccgc ggcgccgagc cccacgacgt
     8881 agccgaccgt ggcggagagc gcacggccga cgggaccgcc cgcgccccac cagatgccgg
     8941 ccgcgctcac gacggccacc acgaggaacg cgacgccgag cccgtcccgg cggtgggcgg
     9001 ggtcgagctc gcgggcacgc ccaaccaccc gtcccagcga ccggccgagc ccggcgatcc
     9061 cgcggccgac ggcgtcgatt ccgcggtcga tcaggtcggg accgcggcgg ggcgccggct
     9121 tgcgggcggc cgggcggcgc gccgtggacc gggcggcggg cttgcgggcg gcaggcttcc
     9181 gcgctgccgg cttccggccg cccgtcaccc gggacgccgc tgccgcggtg cccctgccgc
     9241 cgccggcggt cgtccgtcct gccatggccc cacggtatcc gggacggcgc cccggtccgg
     9301 tgaccgacac ccgccggtcg cacgcggcga gcagccgcgt ccggactgtc gggggtcgct
     9361 gaaacagtga tcgacgtgac ggaccacggg gcacacgcgt tcgacttcat ccacggctcc
     9421 tggcgggtgc acaacacgaa gctgcgcgac accaccgatc cgagctgcga ggagtgggtg
     9481 gagttcgacg ccacgagcga ggtgttcccc gtcctgcgcg gggtcggcca cgtcgaccgg
     9541 atggacgtcc cggatcctcc ggacggcgat ccgttcgagg gcttcacgct gcgcctgttc
     9601 gatccggcgg ccgccacctg gagcatctgg tggagctcca gccgcaaccc cgggcacctc
     9661 gagccgccga tggtcggtgc cttcacgggc gaccacgggg tgttcctcgc ggacgatgtc
     9721 gtgggagggc acgcggtcac cgtgcgcttc gagtggcggg cagacccggc gtcgccccgc
     9781 tggcagcagt ccttctccta cgatgcaggc cggacgtgga ggccgaactg gacgatggac
     9841 ttcagccgcc gagcaggctg atcacgagag cagtcgccgg actccgcgtc attcacgtga
     9901 cgtgagggcg tccaccacga gccccacgcc gatcccgagc agccagacgt ccttggcgaa
     9961 cgggatcccc tgctggctgg ggcgcaggct gccctcctca cgcatgccgg gggtacgcac
    10021 gaacagcccg agcagacccg cggagaacgc cgtcagaccc gcaccggcga gcagcgcggg
    10081 caccgcaggc aggacgagcg cggcgccgag accgatctcg gccgcggcga gcccgcgcag
    10141 gaacatgggc gggtccacct gcttgagcac cgggtaggcg ttgctggcca tgccgtgcag
    10201 cgcgcctgcc gtctgctcgt cggcgctcca tttctgcaac ccggaattga ggatgtacgc
    10261 ccccgcggtc agccgcacag ggagccggcg aagggttgcg gccgagatca tgggctgctc
    10321 ctcggtgtct cgtggagcaa gcggagtacc ccgccggggt caggcgtacc gcctgagcac
    10381 cacccaggtc gtggtgcgga cggtctcccg ccagcccgag acccggtcca gcacggcgtc
    10441 gagctctccg gcgctgggtg ccgccaccag cgcgatcagg tcgaactcgc cggtcaccgt
    10501 ctcgatctcc gtgacgccgg gcagcgcgcc cagccgccgc acgtaaccgg ccacgtcggg
    10561 cgtccggatc acgatgccga cccgggcccg gtgcgccgcg gcgtcccccg gcagcacgac
    10621 gtccgcgcgg tagccgcgga tcacgccgtc gcgctcgagg cgttgcacgc gggcaagcac
    10681 ggccgtgcgg gacagcccga tgctgcgtcc cagcaccgcc atcggggcac gcccgtcagc
    10741 ggcgagaagc atcagcaacc tgcggtccag gtcgtcaatc tgacgatccg tcggcgtcac
    10801 gggcgacatg gtggcacttc gccgaaggac accgacggtc ggcatcgtgc gtcgtcagga
    10861 ttgcgtcaac acacttgact catgcccgcc accgtcaccg tccggcccga acgcgtgc
//
LOCUS       DASRSC010000074         9150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2644090,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000074 DASRSC010000000
VERSION     DASRSC010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9150
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2644090"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VFE39_03665"
     CDS             complement(<1..912)
                     /locus_tag="VFE39_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HET6257727.1"
                     /translation="MSSATTTGTDWTDRILELEVGPVAHGGHCVARSEGRVVFVRHAL
                     PGERVLAVVDEDGGGSYCRADAVEVLDASPWRVPTSCSWARAGGCGGCDLQHADPAAQ
                     RSLKADVLAEQLRRLAGIERPVRVEELPGGALGWRHRVRLAVDDQGRPGLRAHRSHDV
                     LPIADCPLTPAGTLPPVLEHGFEPGSEVEVAVDADGLVHVGDDGPVVQRAAGREWLLS
                     PGVFWQVHPALPDTLAAVVGEWAQAPRGGTAWDLYGGVGLFAAVLAGQVGPDGTVTVV
                     ESSRQAVADGKAALADLPQVGWRVGRVE"
     gene            complement(909..2951)
                     /locus_tag="VFE39_03670"
     CDS             complement(909..2951)
                     /locus_tag="VFE39_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6257728.1"
                     /translation="MSKLGVAVKRVLVGRPQRSDRLAHTLLPKRIALPVFASDAMSSV
                     AYAPEEIFLTLSAAGVAAYAFAPWVGLAVVVVLLTVVTSYRQNVHAYPSGGGDYEVAT
                     VNLGQKAGLTVASALLVDYTLTVAVSISSAAANIGALVPYVAEHKVQFAVVAILALTA
                     VNLRGVRESGIAFAIPTYAFILGIGTMIIWGLTRILLLGDDLRAPSANLHLLAEGDAF
                     TGLALVLLVLRSFTQGAAALTGVEAISNGVPAFRKPKSRNAATTLLLLGTISVAMFSG
                     IIALAMLTGAKVAEFPATQIVDAPPGYVQETLVAQLAEVVFNNFRPGFFFVIIFTALI
                     LVLAANTAYNGFPVLGSILSQDRYLPRQLHTRGDRLAYSNGILVLAGVAIVLVVGFRA
                     DVTRLIALYTVGVFTSFTLSQIGMLRHWNRLLATETDQAARRRMRRSQAINGFGAAMT
                     GIVLVIVLITKFTRGAWIAIAGMAVFFVLMQAIRRHYDRVSAELVAGDTDAVLPSRNH
                     AIVLVSTLHKPTLRAVAYARATRPDVLEAVTVNVDDTDTKRLMRDWEKRKLPVPLKVV
                     ESPYREITKPVLDYVKRIRSSNPRDVVTVFIPEYVVGHWWEQILHNQSALRLKGRLLF
                     QPGVMVTSVPWQLESSKRVNRRPETPPGSSRRGYESVPPREAIPPTPSPATPGGRS"
     gene            3020..3682
                     /locus_tag="VFE39_03675"
     CDS             3020..3682
                     /locus_tag="VFE39_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET6257729.1"
                     /translation="MYVVIMGCGRVGSALSAGLERLGHEVAVIDRDRQAFRRLSPDFR
                     GLQVVGEGYHRDVLIEAGVERAHAFAAVSSGDNSNIIAARVARETFGIEHVVARIYDA
                     KRAAVYERLGIPTVATVPWSTDRLMRMLLPDGVASAWREPTGTVAILPLPVHEEWVGR
                     PVKELEAATGSRVAFIVRFGTGVLPRGDTAVQADDTVYVAAVSGTVSDVTAAAAAPPQ
                     EG"
     gene            3688..4344
                     /locus_tag="VFE39_03680"
     CDS             3688..4344
                     /locus_tag="VFE39_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET6257730.1"
                     /translation="MRVAIAGAGAVGRSIALELVENKHEVMLIERDLANIDPESVERA
                     EWVHADACELASLEDAGMESCDVVIAATGDDKVNLVVSLLAKTEFAVRRVVARVNDPR
                     NEWLFGENWGVDVAVSTPRLLAALVEEAVAVGDLVRLLTFRQGQANLVEVTLTHDTPL
                     AGKPVRALQLPADSALVVILRGGRVIVPQADDALEGGDELLFVATSAVEEDIRAALGY
                     "
     gene            complement(4421..5074)
                     /locus_tag="VFE39_03685"
     CDS             complement(4421..5074)
                     /locus_tag="VFE39_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HET6257731.1"
                     /translation="MTAGPPPDHQAGDGRPQREFPTMLEQMGGVQGVVASTIPVVVFV
                     VVNVVFALQAALIAALAAGVAVAVWRIVRRQQLQPAVSGLFAVGVAAFLAYRTGEARA
                     FYLPGLLFSAALGLAFLVSVLVRWPLAGVVWHGINGDGQSWRRDPRMLRAYTLASILW
                     TVVFAARVAVQGLLYNADEETWLGIARLAMGYPLVGVALLGTVWAVRRARRATPSTP"
     gene            complement(5122..5505)
                     /locus_tag="VFE39_03690"
     CDS             complement(5122..5505)
                     /locus_tag="VFE39_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HET6257732.1"
                     /translation="MAGTDGGAFRRMLRRLTSDVEVLDADDLSEDAEKSGAQRASECA
                     CGQEVTVLGRLRSVEFCPQDAEASLEAELFDGTEGVTLIWLGRRRIAGIEPGRTMRVR
                     GRLAVRDGRKVLYNPYYEICQAQTP"
     gene            5649..6395
                     /locus_tag="VFE39_03695"
     CDS             5649..6395
                     /locus_tag="VFE39_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6257733.1"
                     /translation="MRAVVLPGSGSDDVFVRSVFTEPLRAVGIALQAVAPRHGHDVIE
                     GYRAELDAALDTPGPLLVGGISLGAHVAASWAAKRRTAVGPRLAGLLLALPAWTGPAG
                     DAAAALAARATSAQARTHGVPAAVAAAGAGSPPWLAAELARAWPRYGTGLAAALDAAA
                     AEPAPDAAALRSLAVPAGIAALVDDPVHPLTVAEHWRGVLPRAVLLSAPLALLGVDPA
                     VLGRAAVLAWLRAARAGSPGSNRGHPTISP"
     gene            complement(6604..8580)
                     /locus_tag="VFE39_03700"
     CDS             complement(6604..8580)
                     /locus_tag="VFE39_03700"
                     /inference="COORDINATES: protein motif:HMM:NF023917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3710 domain-containing protein"
                     /protein_id="HET6257734.1"
                     /translation="MARRTRAAVDNGAAADESRTGGALALEPRVDDLPVGGPYDGDDL
                     DPEITDAAGIVDFGAVRVPVPARGTVAVEPTANGRMQAVHVALPEGRLSVSALAAPKS
                     SKLWPELAREIDASLREGGATVRSFPGPWGRELHATSGGATSLFVGVDGPRWMLYGVA
                     TGPTAHADALDVELRRMLRAIVVVRGRAPYPVRTVLPLVVPDHVAEAMAASAASAAAA
                     QAAVPTPPAAPTDQVAAPPAAAAAPPGAPEPSVEQPEAVRPPIEDVPRVRPQPARPRQ
                     PAHAPGEPAAPAPAARERDGRPAREREVAAPLWADPVQRIADADDAGPAGQGFDAGPD
                     RVDFDRFDDVDFDNAPTQVWAALPLEPADQVAEPWRPDPEPDLVQGQGDRRPAADPVP
                     PHTPARGGVPTEPWTFEARAADAVAPEAWAFGPRTERPESVRGWMPGLEPTAAEPGEE
                     APDNADLPTDSWPLASRYRAEPAADVSAEPVWSLDPAGRAQARPEPTPEADGTAATPD
                     APRRGPEESEPRTGRRRALGFGDSVSSTGTPRTLGAEYLIDSGAHALPSNGGRRRAPE
                     PTDDAAVVASGTGRRRAPEPEPLTWPFGGLTAPAIEADHRSDESGLTLERPRRGGRHA
                     APESGVEAPETIPLRVYVAPPDPEQPSGRHRRPD"
     gene            complement(8580..9104)
                     /gene="dut"
                     /locus_tag="VFE39_03705"
     CDS             complement(8580..9104)
                     /gene="dut"
                     /locus_tag="VFE39_03705"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET6257735.1"
                     /translation="MPGPVDAPHHEPLTASPPGWSVEVLLTRLDPGLPIPSYARAGDA
                     GVDLCCTTDIVLPPGERAMVGTGVAIALPAGYAGFVHPRSGLAARAGLSIVNAPGTVD
                     SGYRGEILVCVINHDPREEVHLRRGDRIAQLVVQKVEQVRFVEVADLPGSERGAGGHG
                     STGGHDLLGAGEAP"
BASE COUNT         1271 a         3293 c         3382 g         1204 t
ORIGIN      
        1 ctcgacgcga cccacccgcc agcccacctg cggcaggtcg gcgagcgcag ccttgccgtc
       61 cgcgacggcc tgccgcgagg actcgacgac cgtgaccgtg ccgtcgggtc cgacctggcc
      121 tgcgaggacg gcggcgaaga gcccgacccc gccgtagagg tcccacgccg tgccgccccg
      181 cggcgcctgg gcccattccc cgaccaccgc cgccagcgtg tccgggagcg ccgggtggac
      241 ctgccagaac acgcccggcg acagcaacca ctcgcgcccg gcggcacgct ggacgaccgg
      301 gccgtcgtca ccgacgtgga ccagaccgtc cgcgtccacg gcgacctcga cctccgagcc
      361 cggttcgaag ccgtgctcga gcaccggcgg cagcgtgccc gcgggggtga gtgggcagtc
      421 cgcgatcggc aagacgtcgt ggctccggtg cgcgcgcagc ccgggtctgc cctggtcgtc
      481 gacggcgagc cggacgcggt ggcgccagcc cagtgcccca ccgggcagct cctcgaccct
      541 caccgggcgc tcgatcccgg ccagccggcg gagctgctcg gccaggacgt cggccttgag
      601 ggaacgctgc gcggccgggt cggcgtgctg caggtcgcag cccccgcagc cacccgcccg
      661 cgcccagctg catgaggtcg gcacgcgcca cggcgacgcg tccagcacct cgaccgcatc
      721 agcgcggcag tacgagccgc cgccgtcctc gtccaccacg gccagcacgc gctctccggg
      781 cagcgcgtgc cggacgaaca caacccgtcc ctccgagcgc gcgacgcagt ggccgccatg
      841 agccaccggc cccacctcga gctcgaggat gcggtccgtc cagtccgtgc ctgtcgtcgt
      901 cgcgctgctc acgagcgccc ccctggagtc gcgggggacg gggtcggcgg gatcgcctcg
      961 cgcggcggca ccgactcgta gccgcgccgg gacgagccgg gcggggtctc cggtcgccgg
     1021 ttgacccgct tggacgactc cagctgccac ggcacgctcg tcaccatcac cccgggctgg
     1081 aacagcaacc tgcccttgag ccgcagtgcg ctctggttgt ggaggatctg ctcccaccag
     1141 tgccccacca cgtactcggg gatgaagacg gtgacgacgt cacgtgggtt gctcgaccgg
     1201 atgcgcttga cgtagtcgag caccggcttg gtgatctcac ggtagggcga ctcgacgacc
     1261 ttgagcggca ccggcagctt gcgcttctcc cagtcccgca tcagccgctt cgtgtcggtg
     1321 tcgtcgacgt tgacggtgac cgcctccagt acgtcgggcc gggtggccct ggcatatgcg
     1381 acggcccgca gcgtgggctt gtgcagcgtc gagaccagca cgatcgcgtg gttgcgcgag
     1441 ggcagcacgg cgtcggtgtc cccggccacc agctcggccg acacacggtc gtagtgacgc
     1501 cggatggcct gcatcagcac gaagaacacc gccatgccgg cgatcgcgat ccaggcaccg
     1561 cgggtgaact tcgtgatgag cacgatcacc agcacgatgc ccgtcatggc ggcgccgaag
     1621 ccgttgatgg cctgcgaccg tcgcatgcgg cggcgggcgg cctggtcggt ctcggtggcg
     1681 agcaaccggt tccagtgccg cagcatcccg atctggctca gggtgaacga ggtgaagacc
     1741 cccaccgtgt agagcgcgat gaggcgggtg acgtcggccc ggaacccgac gaccaggacg
     1801 atcgcgacgc ccgccagcac caggatgccg ttggagtagg ccagccggtc gccgcgcgtg
     1861 tgcagctggc gcgggaggta gcggtcctgg gacaggatcg agcccagcac cggaaagccg
     1921 ttgtaggcgg tgttggcggc cagcacgagg atcagggccg tgaagatgat gacgaagaag
     1981 aagcccggcc ggaagttgtt gaacaccacc tcggcgagct gcgccaccag cgtctcctgg
     2041 acgtagccgg ggggcgcgtc gacgatctgg gtggccggga actcggcgac cttcgcgccg
     2101 gtcagcatcg cgagcgcgat gatcccggag aacatcgcca ccgagatggt gccgagcagc
     2161 agcagggtcg tcgcggcgtt gcgggacttc ggcttgcgga acgctggtac gccgttgctg
     2221 atcgcctcca cgccggtgag cgccgcggcg ccctgggtga aggaccgcag cacgagcagc
     2281 acgagcgcga gcccggtgaa cgcgtcgccc tcggccagca ggtgcaggtt ggcgctcggc
     2341 gcgcgcaggt cgtcacccag caacaggatt cgtgtcaacc cccagatgat catcgtgccg
     2401 atgccgagga tgaacgcgta ggtcggaatc gcgaaggcga tgcccgactc gcggacgcct
     2461 cgcaggttga cggctgtgag cgccaggatc gccaccacgg cgaactgcac cttgtgttcg
     2521 gccacgtacg ggaccagggc tccgatgttc gccgccgccg acgagatcga caccgcgacg
     2581 gtgagggtgt aatccacgag cagcgcgctg gccaccgtga gcccggcctt ctgacccagg
     2641 ttgactgtgg ccacctcata gtcaccgccg ccggacgggt aggcgtggac gttctgccgg
     2701 tagctggtga cgacggtcag caggaccacc acgacggcga ggccgaccca gggtgcgaag
     2761 gcgtaggcag cgacgccggc cgccgaaagc gtcaggaaga tctcctcggg cgcgtacgca
     2821 accgaggaca tggcatcgga ggcgaacacg gggagggcga tgcgcttggg gagcagggta
     2881 tgtgcgaggc ggtcgctacg ctgcggcctt cctacgagca cgcgcttgac agcgacgccg
     2941 agcttggaca ccgaaccagc ctatgcggtg cagcatgttg ggaagcgata gcgttgcccg
     3001 cgatccgggg aggcttgcgg tgtacgtcgt gatcatgggt tgcgggcggg tcggatcggc
     3061 gctgtcggcg gggttggagc ggctcgggca cgaggtcgcc gtgatcgacc gcgaccgcca
     3121 ggcgttccgt aggctcagcc cggacttccg gggcctgcag gtcgtcggcg aggggtacca
     3181 ccgcgacgtc ctcatcgagg cgggcgtcga gcgggcgcac gcgttcgccg ccgtgtccag
     3241 cggggacaac tccaacatca tcgcggcgcg ggtggccagg gagacattcg ggatcgagca
     3301 cgtcgtggcc cgcatctacg acgccaagcg ggccgcggtc tacgagcggc tgggcatccc
     3361 gaccgtcgcg accgttccgt ggagcaccga ccggctgatg cggatgctgc tgcccgacgg
     3421 cgtggcgtcg gcgtggcgcg agccgaccgg caccgtcgcg atcctgccgc tgcccgtcca
     3481 cgaggagtgg gtcggccgcc cggtgaagga gctcgaggcc gccaccggct cccgggtggc
     3541 cttcatcgtg cggttcggca ccggcgtgct gcccaggggg gacactgcag tacaggccga
     3601 tgacacggtc tatgtcgccg cggtgtccgg cacggtcagc gacgtcacgg cggcggctgc
     3661 cgcgccgccg caggagggtt gaggacgatg cgcgtcgcga tcgcaggagc gggtgccgtc
     3721 gggcgctcga tcgccctgga gctggtggag aacaagcacg aggtgatgct catcgagcgt
     3781 gatctcgcga acatcgaccc ggagtcggtc gagcgggccg agtgggtgca cgccgacgcc
     3841 tgcgagctcg cctccctcga ggacgcaggc atggagagct gcgacgtcgt catcgcagca
     3901 accggtgacg acaaggtcaa cctggtcgtc tcactgctcg cgaagacgga gttcgcggtg
     3961 cggcgcgtcg tcgcgagggt gaacgaccca cgcaacgagt ggttgttcgg cgagaactgg
     4021 ggcgtcgacg tcgcggtgtc caccccccgt ttgctggccg cgctggtgga ggaggcggtg
     4081 gccgtcggcg acctcgtgcg gttgctcacg ttccgccagg ggcaggccaa cctcgtcgag
     4141 gtcacgctga cacacgacac cccgctggcg gggaagccgg tgcgggcgct gcagctgccg
     4201 gccgactccg cgctcgtggt gatcctgcgc ggcggccggg taatcgtccc gcaggccgac
     4261 gacgcactgg agggcggcga cgagctgctg ttcgtggcga cgtccgccgt cgaggaggac
     4321 atccgcgcag cgctggggta ctagcgccgg tcgcgtcgcc atgacgttgg tcggccggtg
     4381 gggtgctcgg gcggcgaggg ccgcagggtg ccgagggcgt tcagggcgtc gagggagttg
     4441 cgcggcgggc gcggcgtacc gcccagaccg tgccgagcag cgcgaccccg acgagcgggt
     4501 agcccatcgc gaggcgggcg ataccgagcc aggtctcctc gtcggcgttg tagagcaggc
     4561 cctgcaccgc cacccgggcg gcgaagacga cggtccacag gatgctggcc agcgtgtaag
     4621 cgcgcagcat gcgagggtcg cggcgccacg actggccgtc gccgttgatg ccgtgccaga
     4681 ccacgccggc cagcggccag cgcaccagca ccgacaccag gaacgcgagc ccgagcgcgg
     4741 cgctgaacag caggccgggc agatagaagg cccgcgcctc ccccgtgcgg taggcgagga
     4801 acgcagcgac cccgacggcg aacaagcccg acaccgccgg ctgcagctgc tgcctgcgca
     4861 cgatgcgcca caccgcgacg gccacgccgg ccgcgagcgc cgcgatcaga gcggcctgca
     4921 aggcgaacac cacgttgacg acaacgaaca ccacgacggg gatcgtggac gccacgacgc
     4981 cctgcacacc ccccatctgc tcgagcatgg tggggaactc gcgctgcggc ctgccgtcgc
     5041 cggcctgatg gtcaggcggt gggcctgcgg tcactgggct cctacggtca ctgggctcct
     5101 acggtcatcg ggctcctacg gctacggcgt ctgagcctga cagatctcgt agtacgggtt
     5161 gtagagcacc ttgcgcccgt cgcggacggc gagccggccg cgcacccgca tggtgcgccc
     5221 tggctcgatc cccgcgatac gccggcggcc gagccagatc agcgtgacgc cctcggtgcc
     5281 gtcgaacaac tctgcctcga gggatgcctc ggcgtcctgc gggcagaact ccacgctccg
     5341 caatctaccc aacacggtga cctcctggcc gcaggcgcac tcagacgcgc gctgcgcccc
     5401 ggacttctcg gcgtcctcgg acagatcgtc ggcgtcgagg acctcgacgt cgctggtcag
     5461 cctgcgcaac atgcgacgga acgctccgcc atcggtgccg gccatgtctc ccccagctcg
     5521 gtcggcgccc accgtcgggc gccgctaacg tcaccagagt aactcgtacg aaacctcctc
     5581 ggtggtcggc gtccggcgcc cattcgccct atggagccac agactcggca ccgatcgccg
     5641 aacggtcgat gcgggccgtg gtgctgcccg gatcggggtc ggacgacgta ttcgtccggt
     5701 cggtgttcac tgagccgttg cgtgccgtcg gcatcgccct gcaggcggtg gcgccgcgcc
     5761 acgggcacga cgtgatcgag gggtaccgcg ccgaactgga cgccgcactc gatacccctg
     5821 ggcccctgct cgtgggcggc atctcgctcg gcgctcacgt cgcagcgtcg tgggccgcga
     5881 agcggcgcac cgccgtcggg ccccggctgg ccgggctgct cctcgcgctg ccggcgtgga
     5941 ccggaccggc gggcgacgcc gcagccgcgc tcgccgcccg cgcgacgtcg gcgcaggccc
     6001 ggacgcacgg ggtgcccgcg gccgtggcgg cagcgggtgc tggctcgccc ccgtggctgg
     6061 ccgcggagct ggccagggcg tggccgcgat acggcaccgg gctggccgcc gcgctcgacg
     6121 ccgccgcggc cgagccggcc cctgacgcgg ccgccctgcg gtcgctcgcc gttcccgccg
     6181 gcattgcggc gctcgtcgac gacccggtgc acccgctcac ggtggccgag cactggcggg
     6241 gtgtgctccc ccgcgcggtg ctgctcagcg cgccgctcgc gctgctcggc gtcgaccccg
     6301 ctgtgctcgg ccgcgccgcg gtgctcgcat ggctgcgcgc ggcgagagcc ggctcacccg
     6361 gcagcaaccg cggccacccg acgatctcgc cgtgatcccg gggtcaaccg gctcgacagc
     6421 tccccggtat ccgcgtccta gcccgcagac caccaccgag cgtgcgtgca cgtccagccg
     6481 acactcgtac gattccggtc cgctgggcct cccacatcgt gtggctctac cggccaggac
     6541 ccactcctgc ccggcaaccc acgttcacat gtggcgaggc cccagcccgg acctccataa
     6601 cgtctagtcc ggtcggcggt ggcggccgga cggctgctca gggtcgggcg gagcgacgta
     6661 gacccgcagg gggatggtct ccggcgcctc caccccggat tccggggcgg catgccgccc
     6721 gccgcggcgg ggacgctcca gggtgaggcc ggactcgtcc gagcgatggt cggcctcgat
     6781 ggccggtgcg gtgaggcccc cgaacggcca cgtgagcggc tccggctcgg gcgccctgcg
     6841 ccgtccggtc ccggacgcga ccaccgcagc gtcgtcggtg ggctccggag cccggcgcct
     6901 gccgccgttg gacggtaggg cgtgcgcccc ggaatcgatg aggtactccg cgccaagggt
     6961 gcggggtgtg ccggtcgagg acaccgaatc gccgaacccc agcgcccgcc gccgtcccgt
     7021 acggggttcg ctctcctccg ggccgcgacg cggggcgtcg ggggtcgctg cagtgccgtc
     7081 cgcctcggga gtcggctcgg ggcgtgcctg ggcgcgaccc gcggggtcga gggaccacac
     7141 cggctccgcc gacacgtcgg ctgccggctc ggcgcgataa cgggacgcga gtggccagga
     7201 atcggtgggc aggtccgcgt tgtccggtgc ttcctcgcct ggttcggcgg cggtcggctc
     7261 gagcccgggc atccagcccc gcaccgactc cggccgctcg gtacggggtc cgaacgccca
     7321 ggcctccggc gccaccgcgt cggcggcgcg ggcttcgaat gtccacggct cggtcgggac
     7381 acccccacga gccggtgtgt gcggcggcac cgggtcggcc gcgggtctgc ggtccccctg
     7441 tccctgcacc aggtccggct cggggtcggg ccgccacggc tcggcgacct gatcggccgg
     7501 ttcgagcggc agcgcggccc agacctgggt gggtgcgttg tcgaagtcga cgtcgtcgaa
     7561 ccggtcgaag tcgacccggt cggggccggc gtcgaaaccc tggccggccg gtccggcgtc
     7621 gtccgcgtcc gcgatgcgct gcacagggtc ggcccacagc ggtgcggcga cctcgcgttc
     7681 cctggcgggc ctgccgtccc gttcccgagc ggcaggagca ggggcggcgg gctcgccggg
     7741 cgcgtgggca ggctgccgcg ggcgtgccgg ctgcggccgg acacgcggga cgtcctcgat
     7801 gggcggacgg accgcctccg gctgctccac cgacggctcc ggcgcgccgg gcggtgcggc
     7861 agcagcggcg ggcggcgccg cgacctgatc ggtcggggcg gcggggggcg tcggcaccgc
     7921 ggcctgcgcc gccgcggcac tggccgccga ggcggccatc gcctccgcga cgtggtcggg
     7981 caccaccagc gggaggacgg tgcgcaccgg gtatggcgcc cggccccgga cgacgacgat
     8041 ggcgcgcagc atgcggcgca gctcgacgtc cagagcgtcg gcatgtgccg tcggcccggt
     8101 ggccacgccg tagagcatcc agcgcggccc gtccaccccg acgaaaagcg aggtggcgcc
     8161 gccggaggtg gcgtgcagct cacgacccca cggaccgggg aacgaccgga ccgtggcgcc
     8221 accctcacgc agcgacgcgt cgatctcgcg cgccagttcg ggccacagct tcgacgactt
     8281 gggcgccgca agcgcactga ccgagagccg tccctcgggg agcgcgacgt gcacggcctg
     8341 catgcgcccg ttggccgtgg gttcgacggc cacggtgccc cgggccggga ccggcacgcg
     8401 gacggcaccg aagtcgacga tgcccgcggc gtcggtgatt tccggatcca ggtcgtcgcc
     8461 gtcgtacggg ccgccaaccg gcaggtcgtc gacccgcggc tcgagtgcga gtgcgccacc
     8521 ggtgcgcgac tcgtcggcgg ccgccccgtt gtcgacggcc gcacgggttc gcctggccac
     8581 tagggcgcct ctcccgcgcc cagcaggtcg tgtcccccgg tggagccgtg cccgcccgct
     8641 ccgcgctcgg acccgggcag gtccgccacc tcgacgaacc gcacctgctc gaccttttgc
     8701 acgaccagct gcgcgatccg gtcgccgcgg cgcaggtgca cttcctcgcg cggatcatgg
     8761 ttgatcacgc agaccaggat ctcgccccgg tagccggagt cgacagtgcc cggcgcattc
     8821 acgatggaca gcccggctcg ggcggcgagg ccggaacggg ggtgtacgaa cccggcgtag
     8881 cccgcaggca gcgcgatcgc gacgcccgtg ccgaccatgg cgcgctcacc gggcgggagg
     8941 acgatgtcgg tggtgcagca caggtcgaca cctgcgtcgc ccgcccttgc gtacgagggg
     9001 atcggcagtc ccggatcgag ccgggtgagg agcacctcga ccgaccaccc gggcggcgat
     9061 gcggtgagcg gctcatgatg aggagcgtcg acggggccgg gcacgacggt cgaggctacc
     9121 ctcggtcccc gtgagcgagc atccatcgcc
//
LOCUS       DASRSC010000075        14520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4609978,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000075 DASRSC010000000
VERSION     DASRSC010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 14520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14520
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4609978"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFE39_03710"
     CDS             complement(<1..523)
                     /locus_tag="VFE39_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HET6257736.1"
                     /translation="MTEPRDDEYSADAPGVQATADARDAPRHRVRQLLSRTIGGAWAD
                     DIFSESAAAAFWQTLSLPPLLLGLFGVLGYVGDWFGPDTVTAVQHRIIDLTSGVFTRN
                     AVDEIIAPTVAQVLTTAPGEVVSLGFLLSFWSGSSATAAFVDAITRAYGQYQLRNLVW
                     QRVLAVLLYLVGLA"
     gene            complement(700..1473)
                     /locus_tag="VFE39_03715"
     CDS             complement(700..1473)
                     /locus_tag="VFE39_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6257737.1"
                     /translation="MLMTQADIRHLEHLFDERSRLPADDPHQLILRDQLITGYLPLAR
                     NVARKHAYRTENVDDLVQDATLGLINAVDRFEPERGANFLSFAIPTMTGEVLRHFRDR
                     SDTIRLPRRLLEEQGVLRQAVAELTQRLGRAPRPTEIAAHLCLDVESVIEAMEPHHTA
                     RCSSLDEPSGEGPGIDSRFESVLACTDPETELIELRESLGPLLDALSDRDRRILLLRF
                     FGSMTQSGIAQQVGISQMHVSRVLAATLARLRERLTSEL"
     gene            complement(1627..2367)
                     /locus_tag="VFE39_03720"
     CDS             complement(1627..2367)
                     /locus_tag="VFE39_03720"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF and ANTAR domain-containing protein"
                     /protein_id="HET6257738.1"
                     /translation="MPDQISTPVTSAAAAAELQQLLLATENISEFLEELAVLTASVLP
                     GEVFCGITVRRDHQPATVASSDTRASQVDEIQYGHDQGPCLTSLDTGVVVLIEDLADD
                     DRWSAYRVPALAHGVRSSLSLPLRSDGKVLGALNIYATQPRAFGQHEQLAAGRFAEEA
                     SRALALAVRMAERTEMSQHLQNALASRAAIDQALGVVMGQNRCTADEAFDVLRAISQN
                     RNVKLRDVAAEIITAVSGQPPATEARFS"
     gene            2858..3316
                     /locus_tag="VFE39_03725"
     CDS             2858..3316
                     /locus_tag="VFE39_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6257739.1"
                     /translation="MFEFGAFELDDNRDRVDRDAVWQYLSTEAYWARWRERGDVERQI
                     DGAWRVVAAYEKTSGHMVGFSRAISDGVSFAYLADVFVEPAMRGRGLGVELVRFMVDH
                     DPAQTFRWALHTKNAHSLYERYGFGPPDGRYLERPGRGPGTAPGSADEVR"
     gene            3333..3701
                     /locus_tag="VFE39_03730"
     CDS             3333..3701
                     /locus_tag="VFE39_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257740.1"
                     /translation="MQVGRRIGLSQPAAARMVTTLEAADLAHRRAGSGREVSVQLTPA
                     GERAASELLAARGRPLTELLSALGPAQQAALADLLTPLLARLYSQVGNSELLCRLCDR
                     ASCTSGATCPIGQAERERDG"
     gene            3801..3950
                     /locus_tag="VFE39_03735"
     CDS             3801..3950
                     /locus_tag="VFE39_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257741.1"
                     /translation="MVAVAVVLSSLYPVIPVLLGITVLRERLTRHQSGGLAAALTATI
                     LIATP"
     gene            4238..4528
                     /locus_tag="VFE39_03740"
     CDS             4238..4528
                     /locus_tag="VFE39_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257742.1"
                     /translation="MTAQFRVPDVHPPSRDDLGAAARGARSMLPSTKSMLFFGGLAAT
                     AVVGAIEWPVAAAIGVGTALASRGAADPQPRGGAPAQAETSGSTTETGEGRA"
     gene            4624..8973
                     /locus_tag="VFE39_03745"
     CDS             4624..8973
                     /locus_tag="VFE39_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET6257743.1"
                     /translation="MLNLAGSTVGTVVGAVNGVAELVSAATSGIAASSPGRVHVPLRG
                     VHTGRVATTGVEGQLLAHDGVRRAEVNAALGHVMIEFDPDLLTAAEVLDLVREVEQEC
                     GLASEPYAGREHPGSPRRALQEIALFGVHTAGLVYTTLGRAVPAAPIALPASVLALVD
                     TAPRLRRLVASLVGEPVADALLSTGAAASNALGKRPLGLIVDACHRYSLYQESQARRR
                     AWLAWDTMLAGQEGAHRAGAQPVDPRSAQIPPGPVEKIADRAGVIALGGAAALFAATR
                     TTSAVTAALVLGAPKAGRFGREGFAAQLGRDAAARGSLVLDHDVLRRLDRVDAIVLDA
                     EVLLTGRHAIDDVVPLAEEPGSELSELVEHAHDLVDLDRPGRRRTHEGWTVEPLGPEP
                     PGRAAQVAATLAQRTSMVLTLRRGDEPVALIGVSQALDPFAEAVVTAAARAGLVLVAG
                     PWELARRLETDGAVPGAAELTDSVRALQADGHVVALVSRSQHAALAAADIGIGMVPPR
                     QVPPWTADVLTRAASEVCLLLAAVSTAHQVSRRAAQLAVAGSGLGILLAVLGPVPGAA
                     GRASIAVNLAALLALGLGTWSGMSVARRPEPIPADRTPWHAMSAGAVLDALGSARSGL
                     PEPESRRRHREQPAPDDHDEVGLTRATIEELANPLTPALAAGAGVSAATGSILDPLLI
                     TIVLAVNALIGGVQRVGTQRALRTLRHSSASRVHVRRGESEVELRADELVEGDVIRLQ
                     TGDAVPADCRVLSGHDLEIDESSLTGESVPVPKSNRATSARVLADRHSMLYEGTVVAT
                     GRVDGVVVATGDRTEVGRALHQDGEQAPPSGVEVRLRALTARILPISIGAGLALIPVD
                     LLRRRPAGLALSRAVSLAVAAVPEGLPFVATVAELAAARRLSARGALVRNPSTIEALG
                     RVNMLCFDKTGTLTEGRISLRQVSDGTDTATVDEPLPPHLQDIVAAAVRASPFRADPR
                     QVTHQTDRAVLRGARAIGVSAENGHALVEQLDELPFESNRSYHATLWRGHSGIRISLK
                     GAPEVVLPLCSTRRRNGDTQPLDERARAAIESEVHRLAAQGHRVLAVAQRRVPERARL
                     TETDVQDLEFRGLIALVDPVRPTAAQAVATLRAAGVEIAMITGDHPSTAESIAAELDA
                     LNGRTVMIGTELEELTDTQLTDALPRTAVFARVTPAQKARIVRLLQQSGLAVAVTGDG
                     TNDAPAIRLADVGIALGARATAAAREAADVVVTDDRIETITDAIVEGRGMWSSVRDAL
                     SILLGGNLGEIVYTLGTGLAGGSSALNARQLLLINLLTDVLPAMAVAVRPPPDATPDQ
                     LLAEGPERSLGGALTRDIQARAAITATAAALAWLLARPVSTPAQSSTTGLVALVGAQL
                     GQTLAVRGRTPLVAAAVIGSIALLVTAVQVPGLSRVVGSSPLLPHQWGIAVTAAAAAT
                     AAQLVAQQVLTPR"
     gene            9840..9974
                     /locus_tag="VFE39_03750"
     CDS             9840..9974
                     /locus_tag="VFE39_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257744.1"
                     /translation="MRTSALAGQRTKPDQDPELSKIDRMDDVLALVMARRRRPRLTIV
                     "
     gene            complement(10018..10335)
                     /locus_tag="VFE39_03755"
     CDS             complement(10018..10335)
                     /locus_tag="VFE39_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257745.1"
                     /translation="MTRPVIFVGGPLNGQVAERIGEQWDQYRDDGGQPLSREIGEKVM
                     YQQPASERQHYYLAFIAGQREILAAWVYVHLPLMGFYNAAGTWNASLGAAVEAMQAAE
                     ARE"
     gene            complement(10488..11930)
                     /locus_tag="VFE39_03760"
     CDS             complement(10488..11930)
                     /locus_tag="VFE39_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HET6257746.1"
                     /translation="MKTTAGKVRGWTADGVARFLGIPYAAAPFGANRFAPPAPALAWD
                     GVRDAWEHGPTAPAPGYPPPMDQIFTAMVVPGEEYLNVNVWAPDAAEGAPVMVWIHGG
                     AFVNGSNSVPTYDGTAFARDGVVLVGVNYRLGAEGFLLLPGGTANLGLLDQVAALRWV
                     QENIAAFGGDPGNVTVFGESAGAMSIGSLLAMPAADGLFRRAVLQSGGGHHVLSVATA
                     ARIAGYVADEVGVSLEELPQVPPQRLVEAQQALAVQVQTAPDPARWGEVIANAMVYEP
                     VVDGDVLPDVPIRRITSGSASGVDLLVGSTRDEHRLFLAPSGAMDRATDAVLQAVAGG
                     YGLPGDALDEYRVVRPDASPGVLLADMMTDWFFRIPTIRMAEAHAGAYVYEFDWASPV
                     LDGSLGSCHALELGFVFDTLHSASGLVGHEPPQELADAMHRAWVAFATTGDPGWEPYG
                     RERSVRHFGTTVETVRDPRGDTRAIWEGIR"
     gene            complement(12476..13369)
                     /locus_tag="VFE39_03765"
     CDS             complement(12476..13369)
                     /locus_tag="VFE39_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6257747.1"
                     /translation="MTASRIALVTGANQGIGFALVEGLAARMGPDDLVLLTGRNAQRV
                     ADATSLAATAPGTTSRVEGRVLDVTDAEAVARLAAELDERHGGVDIVVSNAAARLTPD
                     RPQSEQAAEFIDVANGGTHAILRSFGPVLRPRGRLIVVASSLGTLGHLDPGLQPLFDG
                     VTLEEVEEAVESWRTAVHDGTAEEKGWPRWLNVPSKVAQVAAVRAVAAQRRATDVPEG
                     TLVAAVCPGLVDTLASRPWFDDFSRAQTPAQAARAVLDLVLADQIDPATYGELVRFGQ
                     VLPWHSGTPARYRSSSTPIKA"
     gene            13441..14376
                     /locus_tag="VFE39_03770"
     CDS             13441..14376
                     /locus_tag="VFE39_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6257748.1"
                     /translation="MDFTDVSLVALRVFREVAERRTLTAAAAALGYTQSAVSRQIASL
                     ERAARTPLLERRHDGVRLTAGGHIVLRRAAAALDQIDATARELAGLPAEAGTVRLGWY
                     PSAGALLVPRAIAILRRTHPAITVISREGSTPALVRALRAGSLDLAVVASAPPFRPPD
                     AESPHLELETLAERSLYLAVPATHPLAGADSVDVTDLRGQRWIASPSTRDQERLGVWP
                     GLDERPDIAHTARDWLAKLHLVAAGCGLTTLPASLLPAVPPGVRVVAVRGGPQEQRRV
                     ILARLPTPLPEPAARLAEAVRAAVIDVSTPPPRPG"
BASE COUNT         2085 a         5276 c         5051 g         2108 t
ORIGIN      
        1 tggccaggcc gaccaggtac agcaggacgg cgagaacccg ctgccagacc aggttgcgca
       61 gctggtactg accgtaggcc cgcgtgatcg cgtcgacgaa cgcggccgtc gccgacgaac
      121 ccgaccagaa gctgaggagg aaaccgaggg agacgacctc gccgggggcg gtcgtgagga
      181 cttgggccac cgtaggcgcg atgatctcgt cgacggcgtt gcgggtgaac acgccgctgg
      241 tgagatcgat gatccggtgc tggacggccg tgaccgtgtc ggggccgaac cagtcgccaa
      301 cgtaaccgag cacgccgaag agtccgagca gcagcggcgg cagcgacagc gtctgccaga
      361 acgccgccgc ggccgactcg ctgaagatgt catcggccca cgcaccaccg atggtgcggc
      421 tcaggagttg gcggaccctg tggcgcggtg catcgcgtgc atcggccgtg gcctgcacac
      481 cgggcgcatc cgccgagtac tcgtcgtccc ggggctccgt catcggacaa ggatgcgcct
      541 gcgttcgcac tgtcgcccgt gccgatgacc ggctccgttc ggggctggtt ctcagggcgc
      601 tggaccccct ccccctgttc gtcatcggct ccgggaggcg atcgcaaacg gcgtcgaccc
      661 ggttctccgg cgatgtccag ctgtctgtcg agtggcggct cagagctcgc tggtgagccg
      721 ctcacggagc ctcgcaaggg tggcggccag gaccctggag acatgcatct gggagatccc
      781 gacctgctgg gcgattccgc tctgggtcat gcttccgaag aaccgcagca ggaggatgcg
      841 gcggtcgcgg tcggaaagcg cgtcgagcag gggcccgagt gactcgcgaa gctcgatgag
      901 ttccgtctcg gggtcggtgc aggcgaggac ggactcgaac cggctgtcga tgccgggccc
      961 ctcccctgac ggctcgtcga gggaggagca gcgtgcggtg tggtgaggct ccatcgcctc
     1021 gatgacggac tcgacgtcca ggcagaggtg agcggcgatc tcggtgggcc ggggggctct
     1081 gcccaggcgc tgagtgagct cggcgacggc ctggcgcagg acgccctgct cctccagaag
     1141 ccggcgcggc agccggattg tgtcgctgcg gtcgcggaag tggcgcagca cctcgccggt
     1201 catggtcggg atcgcgaagg acaggaagtt ggcgccccgc tcgggttcga agcggtcgac
     1261 ggcgttgatg agcccgagtg tggcgtcctg cacgaggtcg tcgacgttct cggtgcggta
     1321 ggcatgcttt cttgcgacat tgcgggcgag cggcaggtac ccggtgatca gctggtcccg
     1381 caggatgagc tggtgcggat catcggcagg cagtcgggat ctttcgtcga acaggtgctc
     1441 gaggtgccgg atgtcggcct gcgtcatcag catgctcgtc ctgtcgtcga tggcactgga
     1501 catcgaggag aggccgcgct gcgggaccga tcgagcacgc tacgccggtc tcacccgagc
     1561 cgccatcacg cgcagcgagg accgatgcct ccggggcccg gcggtaggcg gtacgaggcg
     1621 gagcgatcag gagaagcggg cctcggtggc cgggggttgc ccgctcaccg ccgtgatgat
     1681 ctctgcggcg acgtcgcgga gcttgacgtt gcgattctgg gagatagccc ggaggacatc
     1741 gaacgcttcg tcggctgtac agcggttctg acccatgacc acaccgaggg cctggtcgat
     1801 ggccgcccgg gaggccagcg cattctgcag gtgctgggac atctcggtcc gctcggccat
     1861 ccgcaccgcc agcgccagcg ccctggacgc ctcctcggcg aaccgtcccg ccgcgagctg
     1921 ctcgtgctgc ccgaaggcgc gtggctgggt ggcgtagatg ttgagggcgc cgagcacctt
     1981 gccatcgctt cgcaggggaa gcgagaggga cgagcgcacg ccgtgcgcaa gcgccggcac
     2041 ccggtaggcg ctccaccggt cgtcgtcggc gagatcctcg atcaggacga cgacgccggt
     2101 gtccaggctg gtcaagcacg gcccctggtc atgcccgtac tggatctcgt caacctggct
     2161 ggcccgcgtg tcgctgctgg cgaccgtggc cggctggtgg tcgcgtcgca ctgtgatgcc
     2221 gcagaacacc tcaccgggca ggacggaggc cgtgaggaca gcgagctcct cgaggaactc
     2281 gctgatgttc tcggtggcca acagcagctg ctgcagttcc gcggctgctg cggctgaagt
     2341 gacaggagta ctgatctggt cgggcattgg gtcggacccg tctggaagcg gcgctcccgg
     2401 taacggcaca tcaaagacgg cacgccacgt tccgacgggc tcgggttgcc tccgacccgt
     2461 ccaccatccc acacccacct ggcatagcgg cggagaccgc gtgagattcg acgcggtccc
     2521 gcagggcggc cgtctccgac gtctgcccca ggtgagcggc ccatgtccga gctgctggtc
     2581 cgagtgcgca ccaggccgcc cccgacactg gtccgcatag ccggtgacct cgacaccctc
     2641 tccgcgccac cgctccgcgc cgccctaccc accgcgccgc agggccatct ggttctcgac
     2701 gtctctcgca ctcagttcct gggaccgttc gatctggatc cagtggccgt ccctggacag
     2761 gacgactgcg cagcgaagct ggatggctct cgtgatttca gagcgccccg catgctcttg
     2821 ctcgactcga tgagtgggtg ccacagtccg ctccgtgatc ttcgagttcg gagcgttcga
     2881 gttggacgac aaccgcgacc gggtcgaccg ggacgcggtc tggcagtacc tgtccaccga
     2941 ggcgtactgg gctcggtggc gggagcgggg cgatgtcgag cgtcagatag acggtgcgtg
     3001 gagggtcgtt gccgcctacg agaagacctc cggacacatg gtgggcttct cccgggcgat
     3061 ctcggacggg gtgtcgttcg cctacctggc cgacgtcttc gtcgagccgg ctatgcgcgg
     3121 ccgcggtctc ggcgtcgaac tcgtccggtt catggtcgac cacgatcctg cgcaaacgtt
     3181 ccgctgggcc ctgcacacca agaatgccca ctccttgtac gagcgctacg ggttcggtcc
     3241 gccggacggc cgctatctcg agcgccctgg tcgggggccg ggcacagcac cagggagcgc
     3301 tgacgaggtg cggtagagat cccggcaggt cggtgcaggt cggccggcgc atcggcctga
     3361 gccagcccgc cgccgcccgc atggtcacca cactggaagc agcggacctg gcgcaccgac
     3421 gcgccggctc cggccgagag gtcagcgtgc agctcacccc ggccggcgaa cgggccgcgt
     3481 cggagctcct cgcagcccgg ggacgaccac tgaccgagct cctctccgcc ctcggcccgg
     3541 cgcagcaggc tgccctcgcc gacctgctga ccccgttgct ggcccgtctc tactcccagg
     3601 tcggcaactc ggagctgctg tgccggctgt gtgacagggc gagttgcacg agcggcgcga
     3661 cgtgcccgat cgggcaggcc gagcgcgagc gggacggctg acgtcgtgga cacgttgctc
     3721 gcgctcgcct ccgcagccag ctacggcctg tccgacttca tcggcggctc gtctgctacc
     3781 tgctcgccac caggacgcag ttggtcgcag ttgccgtcgt cctgtcctct ctctatcccg
     3841 tcatccccgt cctgctcggc atcaccgtgc ttcgcgaacg tctcacccgc catcagagcg
     3901 gtggcctcgc cgctgctctc accgcgacga tcctcatcgc gacgccttga cccaacccgt
     3961 gctcgcccgg cccggcgaac agccatccgc catctcacgt ttgcctgctg gtctcccggg
     4021 caacaccccg tttcgcaggc ggtcgagcag agcgaggtgg gtcgtggcgg gtaagccgag
     4081 aaacgcggag aagcaagccg ccgacgcgga tgcgtcgcct gcacaggcgg gcacggccgg
     4141 cgcgggtacg acggggttga ccgaagacgt gcacccgtcg agcccggctg agggacgctt
     4201 cgaggggcac cgctcggcga cggtcaacct gcctttcgtg actgcccagt tccgggtgcc
     4261 ggacgtgcat ccgccgagcc gcgatgatct cggcgccgcc gcgcgcgggg cgcggtcgat
     4321 gctgccgtcc acgaagtcga tgctgttctt cggcggcctc gccgccaccg ccgtcgtcgg
     4381 agcgatcgag tggccggtag cggctgcgat cggagtcggc accgcgctgg ccagccgggg
     4441 cgcggcagac ccgcagcccc gcggcggggc gcccgcgcag gcggagacat ccggttcgac
     4501 gacggagaca ggggagggcc gggcctgacc tgaccctgtc gaggcgagcc gcggcacgag
     4561 ctgcggctcg tcctgttgtg tttgcgaggt cctggtgttt gcgaggtcct ggtgttgcag
     4621 agcgtgttga acctggccgg cagcacggtg ggcaccgtcg tcggcgcggt gaacggagtg
     4681 gccgagctgg tttccgccgc cacctccggt atcgcggcgt catcgccggg ccgcgtgcac
     4741 gttcccctgc gcggggtcca caccggccgg gtggccacca cgggtgtcga gggtcagctc
     4801 ctcgcccacg acggggtgcg ccgtgcggag gtcaacgcgg cgctgggcca cgtgatgatc
     4861 gagttcgacc cggaccttct gacggcggcc gaggtgctgg acctcgttcg ggaggtcgaa
     4921 caggagtgcg gactggcgtc ggagccgtac gcgggccggg aacatccggg atccccgcgc
     4981 cgggcgctgc aggagatcgc tctgttcggc gtccacactg ccgggctggt ctacaccacg
     5041 ctgggtcgag cggtgcctgc cgcgccgatc gcgctcccgg cgtcggtcct cgcgctcgtc
     5101 gacaccgccc cccgcctccg caggctcgtc gcctcgctgg tcggggagcc tgttgccgac
     5161 gcgctgctca gcaccggcgc tgccgcgtcc aacgcattgg ggaagcggcc gctcgggctg
     5221 atcgtcgacg cctgccatcg ctacagcctc taccaggaga gccaggcgcg ccgccgcgct
     5281 tggctcgcgt gggacacgat gctcgccggc caggaggggg cgcaccgagc cggggcacag
     5341 cccgtcgacc cgcgatcggc acagatcccg ccaggtccgg tggagaagat cgccgaccgg
     5401 gcgggggtga tagccctcgg tggtgccgcc gcgttgttcg ccgccacccg aaccacgtca
     5461 gcggtgaccg ccgcgctcgt actcggcgca ccgaaggccg gacgcttcgg gcgggagggg
     5521 ttcgccgccc aactcggccg cgatgcagcc gccaggggca gcctggtcct ggaccacgac
     5581 gtactgcgtc ggctcgaccg cgtcgacgcg atcgtgctgg acgccgaggt cctgctcacc
     5641 ggccgccatg ccatcgacga cgtggtgcca ctcgccgagg agcccggctc cgagctgtcc
     5701 gaactcgtcg agcatgccca cgacctggtg gacctcgacc gtccgggacg acggcgcacc
     5761 cacgagggat ggacggtcga gccgctcggc ccggagccgc ccgggcgggc ggcccaggtc
     5821 gccgccacgc tcgcccagcg gacgtccatg gtcctcacgc tgcgtcgcgg ggacgaaccg
     5881 gtcgccctca tcggcgtctc gcaggcgctc gaccccttcg ccgaggccgt ggtgaccgcg
     5941 gccgcgcggg ccggcctggt cctggtggcc ggtccttggg agctggcgcg acggctggag
     6001 accgacggcg cggtacccgg tgcggccgag ctgacggact cggtacgggc gctgcaggcg
     6061 gacggccacg tcgtcgctct ggtcagccgg agccagcacg ctgcgctcgc cgctgccgat
     6121 atcggcatcg gcatggttcc accgcggcag gtgccgccgt ggaccgcaga cgtcctgacc
     6181 cgggcggcga gcgaggtgtg tctgctgctg gctgcggtga gcacggccca tcaggtcagc
     6241 cgtcgagctg cccaactcgc cgtcgcggga tcaggtctcg ggatcctcct cgctgtgctc
     6301 gggccggtgc ccggcgctgc aggccgagcc tccatcgccg tcaacctcgc cgcactgctc
     6361 gcactcggcc tcggcacctg gtccgggatg tcggtggctc ggcgccccga gccgataccg
     6421 gctgatcgga cgccgtggca cgccatgtcg gccggcgcgg tgctggacgc gttgggcagc
     6481 gcccgctccg gccttcccga gccggagtcg cggcggcgcc atcgtgagca gcccgcgccc
     6541 gatgaccacg acgaggtcgg cctcacccgg gccacgatcg aggagctcgc caacccactc
     6601 acccccgcac tcgccgccgg cgcgggggtc tcggcggcaa ccggctcgat cctcgacccg
     6661 ttgctgatca ccatcgtgct cgcggtgaac gccctcatcg gcggggtgca gcgggtcggc
     6721 actcagcgag cactccgcac cctccggcac agcagcgcgt cgcgggtaca cgtgcggcgt
     6781 ggcgagtccg aggtcgagct ccgggccgac gagctcgtcg agggagacgt catccggctg
     6841 cagaccggtg atgcggtgcc tgcggactgc cgggtgctgt ccgggcacga cctggagatc
     6901 gacgagtcgt ctctgacggg tgagtcggtg ccggttccga agtcgaaccg cgcgacgagc
     6961 gcccgggtgc tcgccgaccg gcactccatg ctctatgagg gcacggtcgt cgcgacgggg
     7021 cgcgtcgacg gggtggtcgt ggcgaccggt gaccgcaccg aggtgggccg cgccctgcac
     7081 caggacggcg agcaggcacc tccctccggg gtcgaggttc ggttgcgcgc actgaccgcc
     7141 cgcatcctcc ccatctcgat cggcgccggc ctcgcgctga tcccggtcga cctcctgcgc
     7201 aggcgaccgg ccgggctggc gctgtcccgc gccgtcagtc tcgccgtggc cgccgtgccc
     7261 gagggcctac cgttcgtcgc gacggtggca gagctcgcgg cggcccgcag gctgtccgcc
     7321 cgcggggccc tggttcgcaa cccgtcgacg atcgaagcgc tcggccgggt gaacatgctg
     7381 tgcttcgaca agaccggcac cctcaccgaa ggccggatca gcctgcggca ggtgtccgac
     7441 ggtaccgaca cggccaccgt cgacgagccg ctcccgcccc acctgcagga catcgttgcc
     7501 gccgcagtgc gggccagccc attccgggcc gacccgcgcc aggtcaccca ccagaccgac
     7561 cgggccgtgc tgcgcggcgc ccgcgccatc ggggtcagcg cggagaacgg acacgcgctc
     7621 gtcgagcagc tcgacgagct gcccttcgag tccaaccggt cgtaccacgc caccctgtgg
     7681 cgcggccaca gcggcatccg gatcagcctc aaaggtgcgc ccgaggtcgt gctgccgctc
     7741 tgcagcaccc gccgtcggaa cggcgatacc caaccgctcg acgaacgagc ccgcgctgcc
     7801 atcgagtccg aggtgcaccg cctggccgcg caaggacacc gcgtgctcgc cgtcgcccag
     7861 cgacgcgttc cggagcgggc ccggttgacc gagaccgacg tccaggatct ggaattccgc
     7921 ggtctgatcg cgctggtcga ccccgtccgc ccgaccgcag cgcaggcggt cgcgaccctg
     7981 cgcgcagcag gcgtcgaaat cgccatgatc accggagacc atcccagcac agccgagtcc
     8041 atcgccgccg aactcgatgc cctcaacggg cgtacggtga tgatcggcac cgagctcgag
     8101 gagctcacgg acacccagct caccgacgcc ctgccccgca ccgccgtctt cgcgcgcgtc
     8161 actccggctc agaaggcgcg catcgtccgg ctgctgcaac agagcggtct cgcggtggcc
     8221 gtgaccggcg acggcaccaa cgacgcgcct gccatccgac tggccgacgt ggggatcgca
     8281 ctcggcgcca gagcgaccgc cgccgcgcgt gaggcggccg acgtcgtggt caccgacgac
     8341 cgcatcgaga ccatcaccga cgccatcgtc gagggccgcg gcatgtggtc ctcggttcgc
     8401 gacgccctgt ccatcctcct cggtgggaac ctcggcgaga tcgtctacac cctcggcacc
     8461 gggctcgcgg gcggcagcag cgccctcaac gcgcgacagc tgctgctgat caacctgctc
     8521 accgacgtac tgcccgccat ggccgtcgcc gtccggcccc cgcccgacgc cacccccgac
     8581 cagctcctcg ccgaagggcc cgaaaggtca ctgggaggtg ccctcacccg cgacatccaa
     8641 gcccgcgccg ccatcacggc gaccgcggcg gccctcgcct ggctgctggc ccgccccgtg
     8701 agcacaccgg cgcagtccag caccacggga ctcgtcgcgc tcgtcggcgc ccagctgggc
     8761 cagaccctcg ccgtgcgcgg gcgcaccccc ctcgtcgccg ccgctgtcat cggctcgatc
     8821 gcactgctcg tcacagccgt ccaggtacct ggcctcagcc gggtcgtcgg ctccagccca
     8881 ctgctgcccc atcagtgggg gatcgcggtc actgctgccg ccgcggccac ggccgcgcag
     8941 ctcgtggcgc aacaggtcct gacaccccgc tgaacctgct cgtcatctca ccgtcatccg
     9001 acatccagcc caatggcgct caccgctcat caccgacacc gatgctggta catgtcgtcg
     9061 gaagggtgat gtcatgacgg agatgctggc cgcggtgttc gtcgcggtag cggcagcact
     9121 gctggagcgg cttgcggtgc gagtcgccag atccctgtgg ggagcgctac ggggtgctgc
     9181 cgcctgatat ccccattcgc gagggacagc gccgcgacat cggccgtgct tcacgggcag
     9241 cagactgcgg gccggtcacc tgtcgacact ctcgcgtccg ggatctctgc gggcgccgtc
     9301 gggctgacca ccacgcagat cgcacgactg ctctcttcat ctcccaccgc gctgttgcaa
     9361 cgcacctcta ccgcgtgttc ggccagcaga atccagcagg gcatcgtcgt agccggcgat
     9421 cctccccgcc atgcccgggc ccgcgcgcgc agcgctacac gctcacccgg tccgggtcca
     9481 gcagctcggg cgcacgccgg ccgccgccgg cgaacgcggc cgccagtagc cgcttgtgct
     9541 gctcggtgtc gcggtggtcg aagtccggca cgagcggggc ggaacatgaa tgccacgatt
     9601 gcagtgcccc gggatgggtg gagggtgacc gccggaacgg ggacgggcct actcacggaa
     9661 cacagcaatg ccgacaggca tttacatcat ctgactcccg cgccgcccac tgctgatcga
     9721 gggccgggac ggaacgccga cgaccagcag gcacgctccc ggcgggacca gcccttcgtc
     9781 tccccgacat cgagcatgat ccccaatgaa gagtgccggt gaagagtgcc ggtcgggaca
     9841 tgcggacctc cgcgctcgcc ggacagcgca caaagcccga ccaggatccg gaactgtcga
     9901 agatcgaccg catggatgac gtcctggcgc tcgtcatggc gcggcgccga cgccctcgcc
     9961 tgacgatcgt gtgatctgaa ccggccggac tgatcggcga gctcccaaga atgggggcta
    10021 ctcccttgcc tcagccgcct gcattgcttc caccgctgcg ccgaggctgg cgttccaggt
    10081 ccccgcggcg ttgtagaagc ccatgagcgg caggtggacg taaacccacg cagcgaggat
    10141 ctcgcgctgc ccggcgatga acgccaagta gtagtgctga cgctcgctgg ccggctgttg
    10201 gtacatgacc ttctcgccga tttccctgga gaggggctgc ccaccgtcgt cgcggtactg
    10261 gtcccactgt tcaccgattc gctcggcaac ctggccgttg agcggtccgc cgacgaagat
    10321 cacgggtctg gtcacggcgg ggatggtcgc atgcctcaca tccggccgac tgccgaccac
    10381 accagccggg ccgctcgtac tcttcgtggg ctcgcactat tcatgggaag ggccaagatg
    10441 gcacgtcgcg cgacgtgtcc accccagcca ggtgagcagg gaccgcctca gcgaatcccc
    10501 tcccagatcg cacgggtgtc gccacgaggg tcgcgcacgg tttcgaccgt cgtgccgaag
    10561 tgccggacgc tgcgctcccg cccgtagggc tcccagcccg gatcgccggt ggtggcgaac
    10621 gcgacccacg cccggtgcat cgcgtcggcc aactcctgcg gcggctcgtg gcccacgagc
    10681 ccggaggcgc tgtgcaaagt gtcgaacacg aaccccagct ccagggcatg gcaggagccg
    10741 aggctgccgt cgaggaccgg cgaggcccag tcgaactcgt agacgtacgc gcccgcgtgc
    10801 gcctcggcca tgcggatcgt gggaatgcgg aagaaccagt ccgtcatcat gtcggcgaga
    10861 aggacgcccg gcgacgcgtc cggccgcacc acgcggtact cgtcgagcgc gtcgcccggc
    10921 aacccgtacc cgcccgcgac cgcctgcagc accgcgtcgg tggcgcggtc cattgcgccg
    10981 gagggcgcga ggaacaggcg gtgctcgtcc cgggtggacc ccacgagcag gtcgacgccc
    11041 gaggcggaac cggaggtgat gcggcggatc ggtacgtcgg gcagcacgtc gccatcgacc
    11101 accggttcgt agaccatggc gttggcgatg acctcgcccc agcgagcggg gtcgggcgcc
    11161 gtctggacct ggacggcgag cgcctgctgc gcctccacca accgctgcgg cggcacctgt
    11221 gggagctcct cgagggaaac gccgacctcg tctgccacgt agcccgcgat ccgcgcggcc
    11281 gtcgccaccg acaggacgtg gtggccgccg ccgctctgca ggacggcccg tcggaacagg
    11341 ccatcggccg cgggcatcgc cagcagggac ccgatgctca tcgcacccgc cgactcgccg
    11401 aacacggtga cgttgccggg gtcgcctccg aacgcggcaa tgttctcctg cacccagcgc
    11461 agcgccgcga cctggtcgag caggccgagg ttcgccgtcc caccggggag cagcaggaag
    11521 ccctcggcgc cgagccggta gttcacgccg accagcacca cgccgtcgcg ggcaaaggcc
    11581 gtgccgtcgt aggtcggcac cgagttcgac ccgttgacga aggcgccacc gtggatccac
    11641 accatcaccg gtgcgccctc ggcggcgtcc ggcgcccaga cattgacgtt caggtactcc
    11701 tcgccaggca cgaccatggc cgtgaagatc tggtccatcg gcggcgggta gccgggcgcg
    11761 ggcgccgtgg ggccgtgctc ccacgcgtcg cggacgccgt cccaggcgag cgcgggggcg
    11821 ggtggagcga accggttcgc cccgaacgga gccgccgcgt acgggatgcc caggaagcgg
    11881 gctacgccgt cggccgtcca gccgcgaacc ttgccggctg tcgtcttcac ctcaggatgc
    11941 atctccgcag tctcgccgag ggcgaccgtc tccgaacagt cggcacacct cgcacgcccg
    12001 gtcgtcgcga tcacaccgag atccgcgacc tccctcagcc ccacgttcgg gcgtccaccg
    12061 ctgtcgcgag gtcgccgaac acgaagggat cgggctcacc ctcggcgagg gagtcgctgg
    12121 ggaacccgag ccggaactcc accgcggcct ccagccgccg tatccggcgg gcaggacgag
    12181 gtcgagctaa cccaggttct cgccggcgca tcggagatcc cgacgtagag gacctttccc
    12241 gcgcgcacgg cgtcgtccag cgcgcgcatg gtctccccgg ccggaggtac tcgatctccg
    12301 ctgtggtgaa agcgctcatg cccccagcct gggcgggccg gccgccccgt ttcgcggcgg
    12361 ctgcggtccc gccggcctgg acactggcca ggagctgacc ggctccccgg cgtggccgac
    12421 cctacggttg ttgatcaaag cgcaggtgtg gttcgcggag atccacaggc gacatctatg
    12481 ccttgatcgg cgtgctggac gaccggtatc gggccggtgt tccgctgtgc cagggcagca
    12541 cctgtccgaa gcggacgagc tcgccgtagg tggcggggtc gatctggtcg gccaatacca
    12601 ggtcgagcac ggcccgcgcg gcctgcgccg gggtctgcgc ccggctgaag tcgtcgaacc
    12661 acgggcggga ggccagcgtg tccactaggc cgggacagac cgcggcgacc agggtgccct
    12721 cgggcacatc ggtcgcgcgg cgttgtgccg cgaccgcccg caccgccgcg acctgagcga
    12781 ccttggaggg cacgttgagc cagcgcggcc agcccttttc ctcggccgtc ccgtcgtgaa
    12841 cggcagtccg ccaggactcg accgcctcct cgacctcctc gagcgtgacg ccgtcgaaca
    12901 gcggctggag cccagggtcc aggtggccga gcgtgcccag cgagctggcc acgacgatca
    12961 accgtccgcg ggggcgtagc accgggccga acgaccgcag gatcgcgtgc gtgccaccgt
    13021 tggcgacgtc gatgaactcg gcggcctgct cggactgcgg ccggtctggg gtgagccgcg
    13081 cggccgcgtt ggacaccacg atgtccactc cgccgtggcg ctcgtcgagc tcggcggcca
    13141 gccgggccac cgcctcggcg tcggtgacgt cgagcacgcg gccttccacc cggctcgtcg
    13201 tgccgggtgc cgtcgcagcg agtgaggtgg cgtccgcgac ccgctgggcg ttgcgtccgg
    13261 tgagcagtac caggtcgtcc ggacccatcc gggcggccag cccctcgacg agcgcgaagc
    13321 cgattccttg gttcgccccg gtgaccaggg cgatgcgtga agctgtcacg acgcccaggc
    13381 taggaacccg gggcccatgc gaccaacgaa agttacgcag ccctggtatg cgtagcagtc
    13441 atggacttca ccgacgtctc gctggtcgcg ctgcgggtct tccgtgaggt cgccgagcgc
    13501 cgcacactca ccgcggccgc ggccgcgctc ggctacacgc agtccgccgt gtcccggcag
    13561 atcgcatcgc tcgaacgggc ggcgcggaca ccgttgctgg agcgacgcca tgacggggtc
    13621 cggctgacag ccggcggaca catcgtgctg cgccgggccg cggcagcgct cgaccagatc
    13681 gacgccaccg cgcgcgagct cgccggcctg cctgccgagg cggggacggt ccgtctcggc
    13741 tggtacccca gtgccggcgc actcctggtg ccccgcgcca tcgccatcct gcgccgaacc
    13801 catcccgcca tcaccgtcat ctcccgcgag gggagcacac ccgcactggt ccgtgcacta
    13861 cgagccggca gccttgatct cgcggtagtg gcctcggcgc cgccgttccg tccgcccgat
    13921 gcggagtccc ctcacctcga gctcgagacg ctcgccgaac gcagcctgta cctcgcggtg
    13981 cccgccaccc acccgttggc cggcgctgac tccgtggacg tcaccgacct gcgcggccag
    14041 cgctggatcg ccagcccctc cacccgcgat caggagcggc tgggagtctg gcccgggctg
    14101 gacgaacgac ccgacatcgc gcacaccgcc cgcgactggc tggccaagct gcatctcgtc
    14161 gccgccgggt gtgggctgac cacactgccc gcctcactcc tacctgcggt accacccgga
    14221 gtgcgggtcg ttgcggtgcg cggcggcccg caggaacagc gacgcgtcat cctggcgcgg
    14281 ctcccaaccc cgttaccgga gccggccgcc cgccttgccg aggccgtgcg cgccgcggtg
    14341 atcgacgtat ccacacctcc tccaaggccc ggctgagcgg gcggaacccg accggcagag
    14401 ggtggcccta cctcggacga acgcctctcc ttcgagcagg ggaacgaaag gacgcggctg
    14461 ctgcgcgcgc ctcgggcgag atgccggtcc acccccctgc gcgtggccgt gacctcgatc
//
LOCUS       DASRSC010000076         5507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1825922,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000076 DASRSC010000000
VERSION     DASRSC010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825922"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(475..1335)
                     /locus_tag="VFE39_03775"
     CDS             complement(475..1335)
                     /locus_tag="VFE39_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6257749.1"
                     /translation="MTDGLGMPEQQVQVEGRLGGARGVELYWQGRLPVGEATGVLLLS
                     HGLGEHSGRYGNVVDALVPDGWAVYGVDHRGHGRSEGRRAHLVRYDDWLADFDAFRRQ
                     VAARHDGLPVFLLGHSMGGQIALAYALEHQDELRGLVLSAPALASNAVPQAAVPVLRA
                     LAKVAPTLRPAGIDPTKISKDPAVVANYRADPLVHHGHPTLGLSTALFGQFDVLPERA
                     RALRIPVLMQYGTLDELVDVSGFRRLESTWGSSDRTVHRYEGLWHEIYNEPEREGPLS
                     DLREWLAAHR"
     gene            complement(1332..3221)
                     /locus_tag="VFE39_03780"
     CDS             complement(1332..3221)
                     /locus_tag="VFE39_03780"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HET6257750.1"
                     /translation="MSTLGLIALVLAAAVVGGLVMLRRRRISAAPSRPKTVADLVRLR
                     AESMPAPVAASVHPGSTDAGAVQVAVLPDSARPAEARVAVPEPRATGTAHPKVGSGSP
                     TSTTTVEEPEAPTPPGGVPLPAQRPPEPSLELELTVDDTPWGRAARMVAVGDGPGSWQ
                     APASVEIVPLRLVPSQGPSSGSPGEEQNDFAGWADWADVDAEEDARAVPPPSAPDLVG
                     FGASGLSTGASAGAAESDPTVRGGSAAGAGRLGSAAEVATARPAAAPAVDTADATATA
                     EQRVAGGEPAAAAPNADQVGVAESPAAGSVATAPLASVPAVSMPTPPADRERDPAVRR
                     ATTPAPAGSSVAAAPFNPVPAVSMPTRPADRERDPAVARAITPEPEGSSAAAAPSRGR
                     PSSVDRAAEQDAADLALLRTFGFVDPSLRPDSAPVVSLVSPDGPALEPRGGGAPQPVR
                     YQAVRRGGAPAGHVAVALLDDRGRAVAGGTAGSDGRGEVLAPASGSFVLVCTAPGYQP
                     GAVALTVAEAPIEAEVLLTRSGSLAGSVHGEDGPIKGARVTLVQDGEIVDSGDTDHDG
                     GYRIEDIGGGEYGLSVVAAGCEPIAILLDVPDEADLRHDVDLEPASVQAGYDRTDDDV
                     VIGQL"
     gene            3489..5435
                     /gene="malQ"
                     /locus_tag="VFE39_03785"
     CDS             3489..5435
                     /gene="malQ"
                     /locus_tag="VFE39_03785"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET6257751.1"
                     /translation="MDTELRELAAAHGVATEYRDGQRRPVQVDPEIVVEVLGLLDVDA
                     RSLEARRAALAAAREAAAAGRLPGTIAARTDRARALPRPGVLVDELGARREVTEIPAG
                     LEPGWYRLETGDQEVTVVVAPPTLAEPPRAWGWMLQLYALHSARSWGIGDLGDLREFT
                     AWTGTEHGAGAVLLNPLHAITPVPPVQPSPYTPSSRRFGTPLALRIPDLDSYSRADGP
                     TRAEVDALRPETAAGRIDHDRVWAAKRAALELLWRSEGRPDPRDAGADLWEFATFCAL
                     AERFGGRWSRWPEELRHPDAPGVAAMRKELAPRVAFHAWVQQQVQAQLAGARDAARDA
                     GVRVVHDLAVGCDPEGADGWALQEVLALGVRVGAPPDAFSQQGQDWGLPPWRPDRLAA
                     TGYAAYRDLLRALLRQADGLRIDHVAGLWRLWWVPPGASADRGTYVHYDAEVMLAVLT
                     LEAHRAGAMVIGEDLGTVEPEVTEGLAERGMLGSSVLWFTRDLDAPGEPLLPPDRWPE
                     DSVATVSTHDLPTAAGFLRGEHVRARAELGLLDDVAAEEAKARIDRLELVELLREQGL
                     VEGENPGEDQLIVAIHALLARSRSRLVLVSPYDVVGEVRQPNLPGTVDQYPNWRLPLP
                     VSLEELREDPRVRVVVDQLRLRRP"
BASE COUNT          732 a         2039 c         2049 g          687 t
ORIGIN      
        1 ccgaccacgg ccgcagcatc acgtcgcccg ccgggaaccg cagcacctcc cagccgcacc
       61 ggctgaggta ggcctgtcgt tcgaggtcgc gcacggcccg ttcgggggtg agggggtcgc
      121 ggctgtcgta ctccacggcg agccgcgccc agcggctccg ccgcggacca gccgcccagc
      181 accccgcgac cgtccatcac aagaaacgcg gcacgtgaac ggcccgcgag gttcacctcg
      241 acgtcggcgc acccgaagat ccgggtgaac aggcggtgaa agtgaggacc ccgcagagcc
      301 ttgcgtgacg aggccggcgg cgacggcggc ggacccccgg aacggtgagc gcagttcggt
      361 cgacgcggcc acaccgacgg cagccggtcc agcgcggttc cacccgaagc caagatcgtt
      421 gtgagatccg ggtatacgcc ctcagcgggg cgcatcgggt ggatctcgcg gcgatcagcg
      481 gtgcgccgcg agccattccc tcaggtccga cagcgggcct tcgcgttccg gctcgttgta
      541 gatctcgtgc cagaggccct cgtaccggtg caccgtccgg tcggacgagc cccaggtcga
      601 ctccagccgc cggaagccgg agacgtcgac gagctcgtcc agcgtcccgt actgcatcag
      661 cacggggata cgcagcgcac gggcacgctc gggcagcacg tcgaactgcc cgaacaacgc
      721 ggtcgacagt ccgagcgtcg gatggccgtg gtgcaccagc gggtcggccc tgtagttggc
      781 gacgacagcg gggtccttgc tgatcttcgt ggggtcgatt ccggccgggc gcagcgtggg
      841 tgccaccttc gcgagcgccc tcagtaccgg gacggcggcc tgcggcaccg cgttgctggc
      901 gagcgcgggt gcggagagca cgaggccgcg cagctcgtcc tggtgctcca gcgcgtaggc
      961 cagtgcgatc tggccgccca tgctgtgccc cagcaggaag accggcagcc cgtcgtggcg
     1021 ggcggccacc tgccggcgga aggcgtcgaa gtcggccagc cagtcgtcgt agcggacgag
     1081 gtgggcccgc cggccctcgg accgcccgtg accgcggtgg tcgacgccgt agaccgccca
     1141 cccgtccggg accagggcat ccaccacgtt gccgtagcga ccggagtgct cgccgaggcc
     1201 gtgggagaga agcagcacgc cggtggcctc cccgaccggg agcctgccct gccagtagag
     1261 ctcgacgccg cgcgcgccgc cgagcctgcc ctcgacctgc acctgctgct ccggcatccc
     1321 cagcccgtcc gtcacagctg cccgatcacc acgtcgtcgt cggtccggtc gtacccggcc
     1381 tggacgctgg caggctcgag gtcgacgtcg tggcgcagat cggcctcgtc aggcacgtcg
     1441 agcaggatcg cgatcggctc acaaccggcg gcgacgaccg acagcccgta ctcacccccg
     1501 ccgatgtcct cgatccggta cccgccatcg tgatcggtgt cgcccgagtc gacgatctcg
     1561 ccgtcctgca cgagagtcac gcgggcacct ttgatcgggc cgtcctcgcc gtgcacagac
     1621 ccggcgagtg accccgatcg ggtcagcagc acctcggcct cgatcggcgc ctcggccacc
     1681 gtcagcgcga ccgctcccgg ctggtaaccg ggcgcggtgc agaccaggac gaagctcccg
     1741 gatgcaggag cgagcacctc acctcgcccg tcggacccgg ccgtgccgcc cgcgacggcc
     1801 cgccccctgt cgtcgagcag cgcgacggcg acgtgccccg cgggagcacc tccgcggcgc
     1861 acggcctggt accgcacggg ctgcggtgct ccaccgcccc gaggctccag cgcagggccg
     1921 tccgggctga ccagcgacac caccggcgcg ctgtccgggc gcaggctcgg gtcgacgaac
     1981 ccgaacgtgc gcagcagggc caggtcggcc gcgtcctgct cggcggctcg gtcgaccgat
     2041 gacgggcgac cccgggaggg agcagcggca gccgaactcc cctcgggctc gggagtgatg
     2101 gccctggcca ccgccgggtc ccgctcccgg tcggccggcc gcgtcggcat tgacaccgcc
     2161 ggcacgggat taaatggcgc agcggcaacc gagctccccg cgggcgcggg agtggtggcc
     2221 ctgcgcaccg ccgggtcccg ctcccggtcg gccggcggcg tcggcatcga caccgccggc
     2281 acggaagcaa gcggcgcagt ggcaaccgat ccagcagcgg gcgactcggc cacgcccacc
     2341 tgatcggcgt tcggcgcggc cgcggcaggc tcgccaccag cgacccgctg ctcggcagtg
     2401 gcagtggcat cggcagtgtc aacggccggc gccgcagcgg gccgggcggt ggcgacttcg
     2461 gcggcggaac cgagacgtcc ggcgcctgct gcactgccgc cccgcaccgt cggatcggat
     2521 tccgccgcac ccgcgctcgc gccggtgctc aggccggacg ccccgaagcc caccaggtcc
     2581 ggggccgagg gcggcggcac cgcccgggcg tcctcctcgg cgtcgacgtc ggcccagtcc
     2641 gcccatccgg caaaatcgtt ctgctcctcg ccgggcgacc ccgacgacgg cccctgcgac
     2701 ggcacgagcc gcagcgggac gatctccacg gaggccggcg cctgccatga cccggggccg
     2761 tcgcccaccg caaccatccg ggcggcccgg ccccacggcg tgtcgtcgac ggtcagctcc
     2821 agctccaggg acggctccgg cgggcgctgc gccggcaatg gaacgccccc cggtggggtc
     2881 ggcgcctcgg gctcttcaac cgtggtggtg ctcgtcgggg atccggagcc caccttcggg
     2941 tgggccgtgc ctgttgcgcg gggctccggt acggccaccc gagcctccgc aggcctggcg
     3001 ctgtccggta gcaccgccac ctgcaccgca cccgcgtcgg tggagccggg gtgcaccgac
     3061 gccgcgacgg gcgccggcat gctctcggcc cggagccgga cgagatcggc gaccgtcttc
     3121 gggcggcttg gtgcggcgga gatcctgcga cggcggagca tgaccagtcc cccgaccacg
     3181 gcggcggcga gaaccaatgc gatcagaccc agcgtgctca tcccggcagt ctccgctccg
     3241 tcaccaccca cgcctcccgc gcggcgtggt gatcatgcgg ccgggaccac catggacgca
     3301 cctgcgctgc cggagtaccg ggcgaatagc ccgcagggaa cctagcactc ctgggatccc
     3361 ctgatcgggt gatggtgggg cgcgtgttac gccagatcag tccaactgcc gccggtggtc
     3421 cgcgcggcgg catcctgaca tctggccctg acgggggtag atgcggcgca gtgggggaac
     3481 ggagcggtgt ggacactgag ctgagggagc tggcggctgc gcacggcgtg gcgaccgagt
     3541 accgcgacgg gcaacggcgc ccggtgcagg tcgacccgga gatcgtggtc gaggtgctgg
     3601 gactgctcga cgtggacgcc cgcagcctcg aggcgcggcg agccgcgctg gcggcggcgc
     3661 gggaggccgc agcggccgga cggctccccg gcactatcgc ggcgcgcacc gaccgcgcgc
     3721 gtgccttgcc gcgcccgggc gtgttggtcg acgagctggg ggcacggcgg gaggtcaccg
     3781 agatccctgc tgggttggaa ccgggctggt accggctcga gacgggcgac caggaggtga
     3841 cggttgtcgt cgctccgccc acgctggccg aaccaccgcg ggcctggggc tggatgcttc
     3901 agctctacgc gctgcactcg gcccgctcct ggggcatcgg cgacctcggc gacctgcggg
     3961 agttcacggc ctggacgggc accgagcacg gcgcaggcgc ggtgctgctc aacccgctgc
     4021 acgcgatcac gcccgtgccg cccgtacagc cgtcgccgta cacgccctcg agcaggcgct
     4081 tcggcacgcc gctggcgctg cggatcccgg acctcgactc ctactcccgc gccgacgggc
     4141 cgacccgcgc cgaggtcgac gcgctccgcc cggagacggc ggccgggcgg atcgaccacg
     4201 accgggtgtg ggcggccaag cgcgccgcgc tcgagctgct ctggcgctcc gagggccgcc
     4261 ccgatccccg cgacgcgggc gcagacctgt gggagttcgc caccttctgc gcactcgccg
     4321 aacgcttcgg cggccgctgg agccgatggc cggaggagtt acggcacccg gatgcacctg
     4381 gcgtcgccgc gatgcggaag gagctcgccc cccgggtcgc gttccacgcg tgggtgcagc
     4441 agcaggtaca ggcccagctc gcgggtgcgc gggacgcagc gcgggacgcc ggggtgcggg
     4501 tcgtgcacga cctggccgtc gggtgcgacc cggagggcgc cgacgggtgg gcgctgcagg
     4561 aggtgctcgc gctcggggtg cgggtgggcg cgcccccgga cgccttcagc cagcaggggc
     4621 aggactgggg gctgccgccg tggcgcccgg accggctcgc cgccaccggc tacgccgcct
     4681 accgcgacct gctgcgcgcg ctgctgcggc aggccgacgg gctgcggatc gaccacgtcg
     4741 cggggctgtg gcggctgtgg tgggtgccgc ccggcgcgtc ggccgaccgc ggcacctacg
     4801 tgcactacga cgccgaggtg atgctggcgg tgctcaccct cgaagcgcac cgggcgggtg
     4861 ccatggtgat cggcgaggac ctcggcaccg tcgagccgga ggtcaccgag ggactggcgg
     4921 agcgcgggat gctcgggtcg tccgtgctct ggttcacccg cgatctcgac gcgcccggcg
     4981 agccgctgct gcccccggac cgctggcccg aggactcggt ggccacagtc tcgacgcacg
     5041 acctgccgac ggcggcgggc ttcctgcgtg gcgagcacgt gcgggcgcgc gccgagctgg
     5101 ggttgctcga cgacgtggcc gccgaggagg ccaaggcgag gatcgaccgg ctggagctgg
     5161 tggagctgct gcgggagcag gggctcgtcg agggcgagaa cccgggcgag gaccagctga
     5221 tcgtcgcgat ccacgcgctg ctcgcccgca gccggtcgcg gctggtgctc gtctcgccct
     5281 acgacgtcgt cggcgaggtg cgccagccca atctgccggg aacggtcgac cagtacccga
     5341 actggcgact gcccctgccg gtttctctcg aggagctccg cgaggatccc cgcgtccggg
     5401 tggtcgtgga ccagctgcgc ctgcgccgcc cgtaggggcg atgtgcacgt ggacctcgtc
     5461 gattacatcg agcgggtcgc cgcatgacca gggcgtgacg gcggacg
//
LOCUS       DASRSC010000077        10442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_353045,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000077 DASRSC010000000
VERSION     DASRSC010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10442
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353045"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(725..1489)
                     /gene="eutC"
                     /locus_tag="VFE39_03790"
     CDS             complement(725..1489)
                     /gene="eutC"
                     /locus_tag="VFE39_03790"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HET6257752.1"
                     /translation="MTGNAAERASGPAATTDPWTVLRRHTAARIGLGRAGDSVPTARL
                     LEFEVAHAQARDAVHTPVDVAAIEAQLGGLPTIAVRSAAPDRTTYLQRPDLGRRLDPD
                     CTTLLVRGEHDVAFVIADGLSARAVHDHAAATLQATLDQLDGWRIAPVILATQGRVAL
                     GDEIGERLGAALIVVLLGERPGLSAPDSLGIYLTAGPRVGRRNSERNCISNVHPPGGL
                     GYRAAAAKLVHLMAAARRLGSTDVGLKDDTPALSEE"
     gene            1557..1919
                     /locus_tag="VFE39_03795"
     CDS             1557..1919
                     /locus_tag="VFE39_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HET6257753.1"
                     /translation="MAQSAGIGNHKGAERAIRATVLGGRLAGGQTANLAAQLPAELAA
                     LLPAEGTGERFGVDEFYRRVAEAEGGECTERQAGQHARATLATLEVALTGHEYDHLAA
                     QLPPDYADLLGTEHVQHH"
     gene            complement(2156..2842)
                     /locus_tag="VFE39_03800"
     CDS             complement(2156..2842)
                     /locus_tag="VFE39_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET6257754.1"
                     /translation="MAASDHRPALVVVFDVNETLSDMAPMAARFAAVGAPEYLAKLWF
                     ASLLRDGFALTAAGAQERFSTLADGALRTVLAGVEMNRDADGAVDHILSGMRELPVHS
                     DVPEGVRALRAAGLRLVTLSNGAASVADQLLSRAGLREEFEALLSVEQIGAWKPAPAP
                     YAYAASTCGVDPGEMLLVAAHPWDIDGAARAGLATAWVNRSGTPYPAYSSPPTHTVAA
                     LDELPGALRR"
     gene            complement(3132..3476)
                     /locus_tag="VFE39_03805"
     CDS             complement(3132..3476)
                     /locus_tag="VFE39_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257755.1"
                     /translation="MLIGPSPRKGTQEGRSDPAALNGQGVLVRLAPTAAGKRLVTRAS
                     VGGQWFRAGTSTSEGVIARFATISDPEGNTIKLIQADEADPDPAFGPRIGELEERRVE
                     VGGTRGGGIEDG"
     gene            3695..4054
                     /locus_tag="VFE39_03810"
     CDS             3695..4054
                     /locus_tag="VFE39_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF296 domain-containing protein"
                     /protein_id="HET6257756.1"
                     /translation="MTGAGITAVGACREATLAYFDPDEMTYQDIRVDQQAEVLSMLGD
                     VAEQSGTGQESGQVEPAVHAHVVLGRRDGTTVGGHLKRAIVWPTLEVVVTESPAHLRK
                     RVDEETGLALISLDEPG"
     gene            4355..4561
                     /locus_tag="VFE39_03815"
     CDS             4355..4561
                     /locus_tag="VFE39_03815"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET6257757.1"
                     /translation="MLLVVGGVFAISAAAWHDLPQVVTNAGVPVGELGFALLLGALAF
                     AGGGGGQNLVQSNWIRDKGFGMGR"
     gene            4579..5118
                     /locus_tag="VFE39_03820"
     CDS             4579..5118
                     /locus_tag="VFE39_03820"
                     /inference="COORDINATES: protein motif:HMM:NF037982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257758.1"
                     /translation="MLKTSYLKRANESTIYFALVWGLVLLAGLSQPIVLLVVSACVGG
                     AMMFLYSALLVVLNRKVLPAEVGITRGRRAALVWAFLLFGVLSVLTIIDEVGELVGAD
                     EIVCWPGAALAVLHSASTRPVPQPSVPRAAGAGVAFPSAALLCLRSLLLPGVAVQLGS
                     RRCRGSLLQTALPPGTRRT"
     gene            complement(5405..5779)
                     /locus_tag="VFE39_03825"
     CDS             complement(5405..5779)
                     /locus_tag="VFE39_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257759.1"
                     /translation="MTLHPDDPGRHRRIDDNGTERRGQREVPGDRPQSPATSPPDRNE
                     PAETAWTTEPPWVLPSIVLGLVALTGSLLLFVLAAIAGLALWALTAPRGDPPGQGRDP
                     FGGRFGGGRHRALTVARPLTGP"
     gene            complement(5929..6078)
                     /locus_tag="VFE39_03830"
     CDS             complement(5929..6078)
                     /locus_tag="VFE39_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257760.1"
                     /translation="MAVLLLGGSRPQAPDLQRWVDGDGPAYDSPPLAALVRRKYAAER
                     TAANR"
     gene            complement(6148..6792)
                     /locus_tag="VFE39_03835"
     CDS             complement(6148..6792)
                     /locus_tag="VFE39_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257761.1"
                     /translation="MASPGQWRLLFAALAVLGLAGFVVGALGFERDDAPSPRMGFDSS
                     GIPPPAAATILPFLGISLLATTPTPSAGHVVLTGVGLVALVTLVVREYRKDRPLMPAQ
                     LIAHTLPVTGTGAAMLAGAGFTALVELAVVHLRQIDGVSPPVIGGVLATQVLGMAAAL
                     WLITRMLPTRWFPVLVLGGLLTIAGPCVRPGPAASGGGGLPACPAAVAGREPRR"
     gene            complement(6858..7076)
                     /locus_tag="VFE39_03840"
     CDS             complement(6858..7076)
                     /locus_tag="VFE39_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257762.1"
                     /translation="MVVAADLIQRVPSRRLTSPVPAAFALASAVAALAPNITWFTAGR
                     TLRGAATGICSSSHSPRWSPVTAPRASR"
     gene            complement(7661..7942)
                     /locus_tag="VFE39_03845"
     CDS             complement(7661..7942)
                     /locus_tag="VFE39_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257763.1"
                     /translation="MKGGSDRALRAVLKRVATTSTAAEIPFAPTGGYASWARGGPERV
                     HDIDFVLVEDDMPLALQVLEGTSVVLTIDGVRLGVAGPSGSAAVSRPAP"
     gene            complement(8292..8921)
                     /locus_tag="VFE39_03850"
     CDS             complement(8292..8921)
                     /locus_tag="VFE39_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257764.1"
                     /translation="MLPRARLEAQPGEAAGPGYIHVIDWLTHWISDSPWTYVVVALLT
                     TADIAVVVPADAVIISAVVLALHGPLSVLGVAGAAVAGAVAGDNILYPPRQPGGAADG
                     RPPVPQRVVEATAGLGTHADAPAPHPADHRGAVRSRRPYRRRCSPRGLLHVPWPRFIV
                     PELVAVLLWVTYDVTPVAFGNALPAWTTVPRVRSATWWTRSPLPGSGDS"
     gene            complement(8884..9339)
                     /locus_tag="VFE39_03855"
     CDS             complement(8884..9339)
                     /locus_tag="VFE39_03855"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET6257765.1"
                     /translation="MTTSGSSGSTGAGGGGGSESSTGELLQALTDDVRSLVQQELRSA
                     QAELTGKARRAGRGAAMLGGAAVLGALATGTSAAVLTRVLEKLFGRTMAALIATALYG
                     GGAAMLANAGVEELRRTLPLLPEHTVDSLREDVRAATGAAPGTSRGSAR"
     gene            complement(9636..>10442)
                     /locus_tag="VFE39_03860"
     CDS             complement(9636..>10442)
                     /locus_tag="VFE39_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6257766.1"
                     /translation="LGEIDVWVNVAFTGSLAFFWDTTPEEYERMTRVTYLGQVNGTRA
                     ALARMRPRNCGVVINVGSAMAYRSIPLQSAYCGAKHAIKGFTEAVSTELAHERSKVRV
                     CMVQLPGLNTPQFSWNLNKMPHHPQPVPPVFQPELPARAIRFLAEHPRRNMWVGISTA
                     YTILGERVAPSLVKYYLARTGVKSQQTTADLPRYGSNVHSPSDDDTDRGAHGSFDRRA
                     HSRDPVLWASMHRSAILSGFAAAATLLGWGRRGTSRDRYAGEGSAGGRPG"
BASE COUNT         1479 a         3697 c         3818 g         1448 t
ORIGIN      
        1 gccgaccgcc atgtccgaca ccagcccgtt cgtcaccccc ggtgctcacc ccagcgagcc
       61 gttgggcggc ggggccggtg acgaacgcga gcgcccgttt ccccggatcg ggcggactcc
      121 cgccgccacc cctgccacct ccggcctcga cgacggcggg ccggccgaca ccgacgacgt
      181 gtaccaggcc agccgcgcgg acccccggcc ggccgccgat cagaacctcg ccgagcgcgc
      241 acacgtccaa cttaacgaac tggccgagca gctccgtggc gctcgacgag atcggcggcc
      301 gccgtcgtcg ccgggtccca cgcgcagacg cgtgtcgcgg tcggcctggc cggggtgtcc
      361 tgtcggatct catagcctgg cggcacagct cggcgctgtg cgggtttcgg gcagccctgc
      421 ggaccgagtg tcgcctcggg tgagaggggt ttcgggcgcc ctgggccggt atggcctggt
      481 caggtcgttg cggggagggt gcagcggctg caaggacctc ttcgagcagg tggtacctgc
      541 atcgcttcac gcgtcaggtt tgccactgcc tcacacccct tggccgggtc ggaggcgtca
      601 ggccaaggcc attccatccg acacggctca gcaacgatct tgcgctgcac ggttgcgcca
      661 ctctcgccca ggccgcggag atcgtcgagc acgaaccagg cgacctcgca tccgcgagat
      721 gcggtcattc ttcgctgagc gcaggggtgt cgtccttgag gccgacgtcg gtgctgccca
      781 gccggcgcgc cgctgccatc aggtgaacga gcttcgcggc cgctgcgcgg tagccgagcc
      841 cgccgggcgg gtgcacgttg gagatgcagt tgcgttcgga gttccggcgg cccacgcgcg
      901 gaccggcggt gaggtagatg ccgaggctgt ccggggcgct gaggcccggc cgctcgccga
      961 gtaggacgac gatcagggcg gcgccgagcc gctccccgat ctcgtcgccg agcgctaccc
     1021 ggccctgcgt cgccaggatt accggcgcga tccgccagcc gtcgagctgg tctagcgtcg
     1081 cctgcagcgt cgccgcggcg tggtcgtgca ctgcgcgggc tgacagcccg tcggcgatca
     1141 cgaaggcgac gtcgtgctca ccgcggacga gcagggtggt gcagtcgggg tcgaggcggc
     1201 gacccagatc cggtcgctgc aggtaggtcg tgcggtcagg ggctgcgctg cggaccgcga
     1261 tcgtcgggag cccgccgagc tgtgcctcga tcgcggcgac gtcgacgggt gtgtgcaccg
     1321 cgtcccgggc ctgcgcgtgc gccacctcga actcgagcag gcgtgcggtg ggcacgctgt
     1381 cgccggcccg gcccagcccg atgcgggccg ccgtgtgccg gcgcagcacc gtccatggat
     1441 ccgtcgtagc cgccgggccg gaggcgcgct ccgcggcgtt gcccgtcacg atgtttccag
     1501 cgcgccgacg agccgggcca gctccgggcc gtgcggcgcc agctcgcgga cccgcagtgg
     1561 cccagagtgc cggcatcggc aaccacaagg gcgccgagcg cgccattcgc gccacggtgc
     1621 tcggtgggcg actcgccggt ggccagaccg cgaacctcgc cgcgcagctg ccggccgagc
     1681 tcgccgcgct cctgcctgcc gagggcaccg gcgagcggtt tggggtcgac gagttctacc
     1741 ggcgcgtcgc cgaggcggag ggcggcgagt gcaccgaacg gcaggccggc cagcacgcca
     1801 gggccacgct cgccacgctc gaggtggccc tgaccggcca cgagtacgac cacctcgccg
     1861 cgcagctgcc gccggattac gccgacctcc tgggcaccga gcacgttcag caccactgag
     1921 cgcgccccgg ccggcaccgc gcctacgacg cccggttttc cggtgcggct cgtcgatgag
     1981 ggggtcgggc tgcacgccgt gcgcatgggg gcccgtacag ggtgctgcgc acgatgaccg
     2041 gatcctgttt cgcgagcccc ggcaaccgcg acggaggggt cgacaaacgc ggacgtgcat
     2101 gcccgtcctt acccgcggtc acaccgccga accggccgga ccgagcgtgc aacgactacc
     2161 ggcggagggc gccgggcaac tcgtccagtg ctgccacggt gtgcgtgggg ggactggagt
     2221 aggccgggta cggggtgccg ctgcggttca cccacgcggt ggcaagccct gcgcgtgccg
     2281 ctccgtcgat gtcccaggga tgtgcggcca cgagaagcat ctcgccgggg tcgacgccgc
     2341 aggtcgaagc ggcgtacgcg tagggtgcgg gagcgggctt ccacgccccg atctgctcca
     2401 ccgagagcag ggcttcgaac tcctcgcgca gccctgccct gctgagcagt tggtcggcaa
     2461 cacttgcggc gccgttggac aaggtaacca ggcgaagccc ggcggcccgc agcgcgcgca
     2521 ctccttccgg cacgtcggaa tgcaccggga gctcccgcat cccactgagg atgtggtcga
     2581 cggccccgtc cgcgtcgcgg ttcatttcga cgccggcgag cacggtgcgc agcgctccgt
     2641 cggccagcgt cgagaaccgc tcctgcgctc cggccgcggt cagcgcgaac ccgtcgcgca
     2701 gcaggctcgc gaaccacagc ttcgccaggt actccggtgc gccgacagcg gcaaagcgcg
     2761 ccgccatcgg cgccatgtcc gacagcgtct cgttgacgtc gaagacgacg accagggcgg
     2821 gacggtgatc cgaagctgcc atgcctgcct ctacccgtgg gcacctcgcg ctaacctgcc
     2881 cggtcgactc cgcccaaggc cttcgacagc gccggaaatc aacccgattc acccgagcgg
     2941 ccctcagcgc ggatcgcagc cgctcagggc ctgcggacga cgtctgagcc aaggctggtg
     3001 gggccgatca ccgagcggtt cacgaggttc ccacgcgcag ggcggtccgc taccggaccc
     3061 cgccgacctt ccttgtctcg tggcggccgt cccgacgcct gcgtgctggc caacacccca
     3121 cgtgcttgcc ttcatccatc ctcgatcccc ccgccacgtg tcccgccgac ctcgacccgc
     3181 cgctcctcca actcgccgat ccgaggaccg aaggccggat cgggatccgc ctcgtcggcc
     3241 tggatgagtt tgatcgtgtt gccctccgga tcggagatcg ttgcaaagcg cgcgatgacg
     3301 ccttcgctgg tgctcgttcc cgcccggaac cactggccgc cgacactggc gcgggtgacc
     3361 agccgcttcc ccgccgcggt cggcgccaat cggaccagta ccccttgccc gttcagggcc
     3421 gcaggatcag atcgcccttc ctgcgtcccc ttcctcggag atgggccgat caacacgtgg
     3481 gatcgactac ccgcgaccgc ctgggcgaac cgtcgcgctg tcgggcgcgg gcatcagccg
     3541 cggcgaaatc gggaacgccg acccgagcgg agccgggagg gccgatgaag agcaggctgc
     3601 tgcacgaggt ggacgggctg cgcacgttcg ccgtcgtgat ggacaaggat gacgacgccg
     3661 ccgaggagct ggtgcggttc gccgcgagaa cgaggtgacc ggcgccggga tcacggcggt
     3721 gggcgcctgt cgggaggcga ccctcgccta tttcgacccg gacgagatga cctaccagga
     3781 catccgggtc gaccagcagg cggaggtgct gtcgatgctg ggcgacgttg cggagcagtc
     3841 cggcaccggc caggagtccg ggcaggtgga gcccgccgtg cacgctcacg tggtgctggg
     3901 gcggcgcgac ggcaccactg tcggcgggca cctcaagcgg gcgatcgtgt ggcccacgct
     3961 ggaggtcgtc gtcaccgaat cgccggcgca cctgcgcaag cgcgtcgacg aggagacggg
     4021 gctggcgctg atcagcctcg acgagcccgg ctgacccccg ggccggggcc gaccttgcga
     4081 tctggcaaca gtcgccgggc acgcgccgac acagcgtttg gaagcaggag tgcgccggga
     4141 accgcccgcc caccgaggtg acgacgagaa cgtcgaggag gcgggcgtgg cgacatccgg
     4201 tggcagcgga cgagctgacg tcgatctgta gtcgacgaag caccttgcga gcccggaggt
     4261 acgggcggtg cccgagcccc cgcgctcgac atgacccccc tggtcgtcta cgtcgcctcg
     4321 agcgcgcgca gatgctcaag gtggccgcgt tctcgtgctg ctcgtcgtcg gcggggtgtt
     4381 cgcgatctcg gccgcggcgt ggcacgacct gccccaggtc gtcacgaacg ccggtgtgcc
     4441 ggtcggcgag ctcggcttcg cgctgctgct cggagcgctg gcgttcgccg gcgggggcgg
     4501 cgggcagaac ctggtgcaga gcaactggat ccgggacaag ggcttcggga tggggcgctg
     4561 agcaggctcg ccgcggacgt gctcaagacg tcctacctca agcgggcgaa cgagagcacg
     4621 atctacttcg cgctggtctg gggcctcgtg ctgctggccg gtctgtccca gccgatcgtc
     4681 ctgctggtgg tctcggcgtg cgtcggcggc gcgatgatgt tcctctactc ggcgctgctc
     4741 gtcgtgctca accggaaggt gctgcccgcc gaggtcggca taacgagggg gcgccgcgcc
     4801 gcgctggtct gggcgttcct gttgttcggc gtgctgtcgg tgctcacgat catcgatgag
     4861 gtcggggagc tggtcggcgc tgacgaaatc gtctgctggc ccggagcggc tctggccgtc
     4921 ctgcattcgg cgagcaccag gccagtgccg cagccgtcag tgccgcgcgc ggccggagcc
     4981 ggtgtcgcgt ttccctccgc cgcactgctg tgtctccgat cgcttctcct gccgggggtg
     5041 gcggtccagc tcggatcgcg ccgatgccgc ggatctttgc tgcagacggc tctcccgccg
     5101 ggaactcgcc gtacctgacg tcagggggtg atcgggtcga ctgcgtctac ctcgtccccg
     5161 acaccaccag cggcgccgag gaggtggcca cgaccgacac catcccggcg ctcagcgcct
     5221 gacaagcaag aagatcacgc atcaaccgtc acacaccacg cccgaggact tgaccgggat
     5281 cgaacgaccg ggtgttgttc gatgcgtctg ccgcggggca ggcggcgtga caggttgctg
     5341 agctgcggaa gctctggctc cgccgcacat cacgagtcag ctcgggtatc gggcgatgac
     5401 agggtcatgg tccggtcagg ggtcgggcca cggtcagggc acgatgccgc ccgcccccga
     5461 accggccgcc gaacgggtcg cgcccttgcc cgggcgggtc tccccgaggt gcggtcagcg
     5521 cccacagcgc gagcccggcg atggcggcga ggacgaacag cagcagcgat ccggtgaggg
     5581 cgaccagccc cagcacgatc gacggcagga cccacggagg ctccgtcgtc catgcggtct
     5641 ctgccggctc gttccggtcg ggcggcgacg tcgcggggct ctgcgggcgg tccccgggca
     5701 cctcgcgctg accgcgacgc tcggtcccgt tgtcatcgat acgcctgtgc cgaccggggt
     5761 cgtcggggtg cagggtcatc ttcatcacct cctccggtcc cggcataccc ggcgaggcgt
     5821 tcgtattggg actggcgccg ttttcgtaag aacgtcgtca ggtcgctccg gctcacgccg
     5881 ccaatctcca ggtcgcgcca gggccggagt cgtgggcgaa cgacgatgtc atcggttcgc
     5941 ggcggttcgc tcagccgcgt atttccgccg cacgagcgcc gccagcggcg gcgagtcgta
     6001 cgcgggtccg tcgccgtcca cccagcgctg caggtcaggc gcctgcggtc gggaaccgcc
     6061 gagcagcaac acagccatga acaggacggt gcccagcgcc gccgcgacgg cgatgaggac
     6121 cgctgcgggc cgatgctgtc ggcggagtca gcgccggggc tcgcgacccg caacagcagc
     6181 ggggcaagca ggaaggccgc ctccgcccga agcagctgga ccagggcgaa cgcagggccc
     6241 agcgatcgtg agcaggccac cgaggacgag cacggggaac cagcgggtgg gcagcatgcg
     6301 cgtgatgagc cagagcgccg cggccatccc cagcacctgg gtggccagca ccccgccgat
     6361 gacgggcgga ctcaccccgt cgatctgccg gagatggacc acggcgagct cgacgagggc
     6421 cgtgaagccg gcgcccgcca gcatcgccgc gccggtcccc gtgaccggaa gggtgtgggc
     6481 gatcagctgc gcgggcatca gcggtcggtc cttgcggtac tcccgcacga cgagggtgac
     6541 gagtgcgacg agcccgactc ccgtcaggac gacgtgcccg gcagaggggg tcggtgtcgt
     6601 ggccaggagg ctgatcccga ggaacggcag gatcgtcgcc gcggccggtg gcggaatgcc
     6661 cgaggagtcg aatcccatcc gcggagaggg ggcatcgtcc cgctcgaacc ccagcgctcc
     6721 gacgacgaac ccggcgagcc ccagcacggc cagcgcggcg aagagcagcc gccactggcc
     6781 ggggctcgcc atcagccccc cgatcgtcgt gccgaccgcc gagccgccga agagaccgag
     6841 gttgaccagc gcgacggtca gcgggatgcg cgcggcgccg tgaccggtga ccagcggggg
     6901 gagtgcgacg acgagcagat cccggtggcg gcgccccgga gcgtcctccc ggccgtgaac
     6961 caggtgatat tcggcgccag cgccgcgacg gccgatgcga gtgcgaacgc ggccggtaca
     7021 ggcgaggtca gccggcgcga cgggacgcgc tggatcaggt ctgccgccac caccacgcag
     7081 agcgcgtagc ccgcgttggc gagcccgttc gcgatctgca gtccggcgct actcgcaccc
     7141 agctcgggcc ccaggaccgg tgtgagcggg agggacgccg tcgacaggac gacgaaggcc
     7201 tcagcccgag caacgccatg gccaccgcgg tggaccagcg gccggcgagc gggatcgtcc
     7261 gcagcgctcg ggtcaccacc ggcccactcc ggacccgcac cggatggccg ggtggaccgg
     7321 gtcgtcgcag ccgtgcctga tcagacgcgg gggtcgatgg ggcctggcgg cggaggcggc
     7381 acgggtggcg ccggttccgg tcccggccta ggaggtggct ccggtggtgg gatcggcggt
     7441 gtgggttcgg gtccggggcc ggtcggcggt tccggtgtcg ggaccggcgg tgcgggctcc
     7501 ggtcccggtg gcggcgtcgg cggcactggc ggcggctgcg ggtgcgggtg cggcatgggc
     7561 ggctcgcgtg gcaccggtgg tggctcggtg acggtgggga caggggcgct ggcatccatg
     7621 gtgcgggcct acccgcgtcc gtcgccaagg actaatgccg tcacggtgcc gggcgggaaa
     7681 ccgccgccga acccgacggt cccgcaacgc cgaggcgtac cccgtcgatc gtcagcacga
     7741 cggaggtccc ctcgagcacc tgcagggcga gcggcatgtc gtcctccacc agtacgaagt
     7801 cgatgtcgtg cacacgttcc ggcccgcccc gcgcccagga ggcgtagccg cccgtgggcg
     7861 cgaacgggat ctccgccgcc gtggaggtcg tggccacccg cttgagcact gcgcgcagcg
     7921 cgcggtcaga cccccctttc acacgactca gtcaaccggc agatcgccct ccaacccccg
     7981 tccgccgatc aacgaggatc accggctacc accgcacacg tggcggcgac gcgcgcgcgg
     8041 atcccctgcc ctcctggacg gcggcaccga tggcctgcgc cggccaggcg agctccccga
     8101 ggaccggatc ggcgcccttg tggactcccg ggcgccggcg aggtcgggtg tccgaggtga
     8161 ccgcgcactg gcgctgcttc gcggtcggtg agctcggcct caatgctctg tggggcgggt
     8221 gccgacggcc acgagggccc cgatcgtcgc tgctcctgct gggtgggtga ccgtcgtccg
     8281 ccgtgcatgg gtcagctgtc gccactgccc ggcagtggcg agcgcgtcca ccacgtcgca
     8341 gaacgcaccc gcgggaccgt cgtccacgcg ggcagcgcgt tcccgaacgc caccggcgtg
     8401 acgtcgtagg tgacccagag gagcaccgcg accagctcgg gaacgatgaa ccggggccag
     8461 gggacgtgca gcaacccccg cggcgaacat cgtctgcggt acggccgacg ggaacgaacc
     8521 gccccgcgat gatcagcagg atgtggtgcc ggtgcatctg cgtgcgtgcc cagtccagcc
     8581 gttgcctcga ccactcgctg cggaacaggc gggcgaccat cggctgcccc gcccggctgc
     8641 cgaggagggt agaggatgtt gtccccggcg acggccccgg cgactgcggc tccggccacg
     8701 cccaggaccg acagcggccc atggagcgcc agcacgacgg ccgagatgat cacagcgtcg
     8761 gcggggacga cgacggcgat gtcggcggtg gtcaggaggg cgacgaccac ataggtccac
     8821 ggggagtcgg agatccagtg ggtgagccag tcgatcacgt ggatgtagcc cggtcccgcc
     8881 gcctcaccgg gctgagcctc gagacgtgcc cggggcagca ccggtggcgg cccggacgtc
     8941 ctcccgcaac gagtcgacgg tgtgttcggg aaggagcggc agcgtccgcc ggagctcctc
     9001 gactcccgcg ttggcgagca tcgccgcacc gccgccgtac agcgcggtcg cgatcagggc
     9061 ggccatcgtg cggccgaaga gcttctccag gacgcgggtg agtaccgcgg ccgacgtgcc
     9121 ggtggccagc gcgcccagca ccgccgcgcc cccgagcatc gccgcccccc tcccggcccg
     9181 gcgcgccttt ccggttagct ccgcctgcgc actgcggagc tcctgctgca cgagcgagcg
     9241 cacgtcatcg gtgagggcct gcagaagctc gcccgtggag ctctcggagc cgcccccgcc
     9301 gccggccccg gtcgatccgc tcgaacccga tgtggtcatg agaaggtgcc ttccgtcgtg
     9361 acgtcctgcc gtctccgcgc gaggcgaagc cggtccgtct ccccggtgtc ccccgcgaaa
     9421 ccgggcggcg aacgtgtccc cggccgcgga ctacggctgc gggttacggg gtagccacgg
     9481 cacatgcggc gggctcgact gggcgtgatc gtcatggtgc tggtcgtccc cctactgggt
     9541 gcctgcggcg gcgccgacac cggaagcccg tcggcaccgc cggatctcac tccacttccc
     9601 ggctgagcgc aggctcgcga accgctcgga cctgctcatc ccggccgacc gcccgcagac
     9661 ccctcgcccg cgtacctgtc acggctcgtg ccccgccggc cccagccgag cagggtggca
     9721 gccgcggcga aaccggacag gatcgccgac cggtgcatgg aggcccacag caccgggtcg
     9781 cgggagtggg cgcgccggtc gaacgacccg tgggctcctc ggtcggtgtc gtcgtcggag
     9841 ggggagtgca cattggagcc gtaccgaggc aggtcggccg tcgtctgctg ggacttcacg
     9901 cccgtgcggg ccaggtagta cttgacgagc gacggggcca cccgttcccc caggatcgtg
     9961 tacgcggtag agatgcccac ccacatgttc cggcgcgggt gttcggcgag gaaccggatc
    10021 gcgcgggcgg gcagttcggg ttggaagacg ggtggcaccg gttgcgggtg gtgcggcatc
    10081 ttgttgaggt tccagctgaa ctggggtgtg ttgagcccgg gcagctgcac catgcagacc
    10141 ctgaccttgc tccgctcgtg tgcgagctcg gtgctcaccg cttcggtgaa gcccttgatc
    10201 gcgtgtttcg cgccgcagta ggcggactgg agcgggatcg agcggtaagc catggcggag
    10261 ccgacattga tcaccacacc gcagttgcgg gggcgcatcc gcgcgagtgc cgcccgggtg
    10321 ccgttgacct ggcccaggta ggtcacccgg gtcatccgct cgtactcctc cggtgttgtg
    10381 tcccagaaga aggccaggga gccggtgaag gcgacattga cccagacgtc gatctcgccg
    10441 ag
//
LOCUS       DASRSC010000078         4531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5266030,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000078 DASRSC010000000
VERSION     DASRSC010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4531
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5266030"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VFE39_03865"
     CDS             complement(<1..650)
                     /locus_tag="VFE39_03865"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HET6257767.1"
                     /translation="MAKIVSLPQAVSDLVHDGDTVALEGFTHLIPFAAGHEIIRQGRR
                     GLTLVRMTPDLIYDQLIGAGCAAKLVFSWGGNPGVGSLHRMRDALATGWPAPLEIEEH
                     SHAGMANRYVAGASGLPFAVLRGYAGTDLPTHTSTISTVECPFTGERLAAVAALNPDV
                     TVVHAQRADRAGNVQLWGITGVQKEAVLAARRSLATVEEIVDELTPVPGAVVLPAWV"
     gene            1024..1395
                     /locus_tag="VFE39_03870"
     CDS             1024..1395
                     /locus_tag="VFE39_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HET6257768.1"
                     /translation="MRGLGELEAMVMDILWSSPRPHRVRDVLEELTPQRKLAYTTVMT
                     VLDNLHRKGWVQREMDGRAYHYQPSATREEVTARALRDLLDASGDVETVLLHFARSVS
                     ERESAVLRDALGEAPDGADRR"
     gene            1392..2354
                     /locus_tag="VFE39_03875"
     CDS             1392..2354
                     /locus_tag="VFE39_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HET6257769.1"
                     /translation="MTIAIALLLGSAIFALTAPRLLRHMAAMAVDPVAVIVGWMLAVL
                     GVAGTACAGLVIMMVPTYAALTPWAHLVGCPWWAVVQDAPEFVAYDLAGWIAAAAIGA
                     AVLRLGWVAVQHARARRDRVRGRLDVLRMTGTTVPCGDGGPPTLWLPSERALAFSLAG
                     RPGTIVVTEGLRRHLSPDGVAAVLAHERAHLRGRHHLITAWAEVLSVAFPFVWLFRAA
                     SPALREQVEVAADIAAVRSSGRDALRAALLDVDGAGAPEVALAMARDAVDLRLRYLAA
                     VSGPPSRVGRLSRCGTLGTLFAVTPLFAATVLLGVISALATALS"
     gene            2465..3295
                     /locus_tag="VFE39_03880"
     CDS             2465..3295
                     /locus_tag="VFE39_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6257770.1"
                     /translation="MTRFPLPTGPVGRLGFGTMRLTGSGVWGPPTDPDGALAVLRLAA
                     DLGVTLFDTADSYGPEVAEDLLRQALHPYDGITVATKAGFLRTGPGKWHTSGRPEYLR
                     QQCELSLRRLGVERIDLFQLHRVDPWVPADEQYGLLADLQTEGKVAAVGLSEVDVEQI
                     EHAAAIVDVATVQNRYNILDRKSDAVLRYCTELGIGFIPWAPIAAGRLAEPGGPVAEV
                     AAHLGATPSQVSLAWLLQRSSVMLPIPGTSSAKHLEENVRAADVVLDQDSVQKLDDAA
                     "
     gene            complement(3379..>4531)
                     /locus_tag="VFE39_03885"
     CDS             complement(3379..>4531)
                     /locus_tag="VFE39_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6257771.1"
                     /translation="SEHLASRTEVGEILLRSGVPTAVLQAAVIIGSGSASFEMLRYLT
                     ERLPVMLTPRWVRNRIQPIAVRDVLRYLLGAATLPSEVNRTFDIGGPDVLTYLDMMQR
                     YAAVAGLPWRRIVPVPVLTPRLSAHWVNIVTPVPKGIADPLIESLVHEVVCSEQDILG
                     YVPDPPEGRIGYDRAVQLALARISGGEVETRWSGATVPDMPSDPLPSDPRWSGGTVYL
                     DEREQHSDAEPAALWAVVTGIGGERGWYSFPLAWAVRGWMDRLVGGVGLRRGRRDPDR
                     LQTGDALDWWRVEQLRDDHANERTLRLRAEMKVPGRAWLEMTVSPEAGGSRYGQRAVF
                     IPRGLAGHLYWWSVAPFHGIVFGGMVRNITRTAERVGLQRQPGKAADSA"
BASE COUNT          628 a         1643 c         1597 g          663 t
ORIGIN      
        1 acccatgccg ggaggacgac cgctcccggc accggcgtca gctcgtcgac gatctcctcg
       61 acggtggcca acgagcgccg cgccgcgagc accgcctcct tctgcacgcc ggtgatgccc
      121 cacagttgga cgttgccggc gcggtccgcg cgttgcgcgt gcaccaccgt gacgtccggg
      181 ttgagcgcgg cgacggcagc gaggcgctcc ccggtgaagg gacactcgac cgtcgagatg
      241 gtcgacgtgt gggtgggcag gtcggtaccg gcgtagccgc ggagcaccgc gaacggcagg
      301 cccgatgcgc cggcgacgta ccggttggcc atcccggcgt ggctgtgctc ctcgatctcc
      361 agcggagcgg gccaccccgt ggcgagggcg tcccgcatcc ggtgcagcga acccaccccg
      421 gggttaccgc cccacgagaa cacgagcttg gccgcacagc cggcgccgat gagctggtcg
      481 tagatcaggt ccggcgtcat ccggacaaga gtgaggccac gccgcccctg ccgaatgatc
      541 tcgtgcccgg cggcgaacgg gatgaggtgt gtgaagcctt ccagcgccac ggtgtccccg
      601 tcgtgcacaa ggtcggacac cgcctgcggc aacgacacga tcttggccac gtgcgaacgc
      661 tagtgggcgc ggcgagaccg ggcaatcgaa ggcgggcagc gaccgagcgt cgacgatcac
      721 gggcccctgg tccggtggcc ggagtgctgg gtccggggtc cggggaacgg acgcgccgcc
      781 gtcgcgtccg ttccccgccc gacctcgtgg aggagatgcg cgcacgagcg ctcccgaggc
      841 gatggacggc ggtccgatgg tgcccggacc cggaccgtaa ggtgatcgcg gtcgacttcg
      901 tcacgtgatg tcgtgtcgtg gggtaacgcg gcggcggatt gtccgattcg tcgcgcatgg
      961 cctgcgacca gggacgacgc ctttgggtgg cgggcggtct tgtgctgcgg ggcgggagga
     1021 catgtgcgag gtctgggtga actcgaagcg atggtcatgg acatcctctg gtcctcgccc
     1081 agaccgcaca gggtgcgcga cgtactcgag gagctcacac cgcagcgcaa gctcgcctac
     1141 acgacggtca tgaccgtcct ggacaacctg caccgcaagg ggtgggtgca gcgcgagatg
     1201 gacggccggg cctaccacta ccagcccagc gccacccgcg aggaggtcac cgcccgcgcg
     1261 ctgcgcgacc tcctggacgc ctcgggtgac gtcgaaaccg tgctgctgca cttcgcccgc
     1321 tccgtgtccg agcgggagtc cgccgttctg cgggatgcgc tcggtgaggc gccggacggc
     1381 gcggaccggc gatgacgatt gcgatcgcac tgctgctcgg ctccgcaatc ttcgccctga
     1441 ccgcgcctcg gctcctgcgc cacatggccg ccatggcggt cgacccggtg gccgtcatcg
     1501 tggggtggat gctcgcggtg ctcggcgtgg ccggcaccgc atgcgccggc ctggtgatca
     1561 tgatggtgcc gacgtacgca gcgctgacac cgtgggcaca cctggtcggg tgcccgtggt
     1621 gggctgttgt gcaggacgcc cctgaattcg tcgcatacga cctggcgggc tggatcgccg
     1681 ccgcggccat cggagccgcc gtgctccgtc tcggctgggt cgctgtgcag cacgctcgtg
     1741 cccggcgcga ccgcgtgcgc ggccggctcg acgtgctgcg catgaccggc accaccgtcc
     1801 cctgtggcga cggggggcca cccacgctgt ggcttccctc cgaacgggcg ctggcattca
     1861 gcctcgccgg acggccgggc accatcgtcg tcaccgaggg cctgcgccgg cacctgtcgc
     1921 ccgacggcgt cgcggcagtc ctcgcgcacg aacgcgccca cttgcgcggc cggcaccacc
     1981 tgatcaccgc ctgggccgag gtgctctccg tcgcgttccc gttcgtctgg ctgttccggg
     2041 ctgcctcgcc tgcgttgcgc gagcaggtcg aggtcgctgc cgacatcgcg gccgtgcgct
     2101 catccggacg ggacgcgctg cgcgccgcgc tgctggacgt cgacggggca ggggcgccgg
     2161 aggtcgcgct cgcgatggcc cgggatgccg tcgacctccg gttgcgctac ctggctgccg
     2221 tgtcagggcc gccctcgcgg gtcggcaggc tctcgcgctg cggaaccctc ggcacgctgt
     2281 tcgccgtcac gccgctgttc gccgctaccg tgctgctcgg tgtgatatcg gcgctggcca
     2341 ccgcactctc gtgagatccg ggtgacatca cgggaggcgc cgatcacgaa ctgcggcgcc
     2401 cggttcgtca cggtgcgatg gtgacgtcgt ggtcaccctt catgacaccg gtcccgcccc
     2461 caccatcact cgcttccccc ttccgaccgg cccggtgggc cgcctcggtt tcgggacgat
     2521 gcgcctcacc ggatccggcg tgtgggggcc gcccaccgac ccggacggcg ctctggcggt
     2581 cctgcgcctc gcggccgacc tcggcgtcac cctgttcgac acggcggact cctacggccc
     2641 cgaggtcgcc gaggatctcc tgcgccaggc gctgcacccc tacgacggca tcaccgtcgc
     2701 caccaaggcg ggcttcctgc gcaccggacc cggcaagtgg cacacgagcg gccgcccgga
     2761 gtacctgcgg cagcagtgcg agctgagcct gcgcaggctc ggcgtcgagc ggatcgatct
     2821 gttccagctg caccgcgtcg acccgtgggt gccggccgac gagcagtacg gcctgctcgc
     2881 cgatctgcag accgagggca aagtcgccgc ggtcggcctc tccgaggtcg acgtcgagca
     2941 gatcgagcac gccgccgcaa tcgtggacgt ggccacggtg cagaaccgct acaacattct
     3001 cgaccgcaag agcgacgccg tgctgcggta ctgcacggag ctcggaatcg gcttcatccc
     3061 ctgggccccg atcgcggccg gccgcctcgc cgagcccggc ggtcccgtgg ccgaggtcgc
     3121 cgcccacctc ggcgccaccc catcgcaggt gtcattggcg tggctgctgc agcgctcgtc
     3181 ggtcatgctg ccgataccgg gcacgagcag cgcgaagcac ctggaggaga acgtccgcgc
     3241 agccgacgtg gttctggacc aggactcggt ccagaagctc gacgacgccg cctgacccag
     3301 ccccgcgagt cgcccggatc aggtccgcga gtcgcccgga tcaggcctgc gagtcgcccg
     3361 gatcgggtcc cggtcgccct acgcggagtc ggcggctttg ccgggctgcc gttgcaggcc
     3421 gacgcgttca gcggtccggg tgatgttgcg gaccatcccg ccgaacacga tcccgtggaa
     3481 cggggccacc gaccaccagt acaggtggcc ggccaggccg cgagggatga acacggcccg
     3541 ctgcccgtaa cgcgatccgc cggcctccgg cgacacggtc atctcgagcc acgcgcggcc
     3601 cggcaccttc atctccgcgc gcagccgcag tgtccgctcg ttggcgtggt cgtcccgtag
     3661 ctgctcgacc cgccaccagt cgagagcgtc gccggtctgc aggcggtcgg gatcgcggcg
     3721 gccgcggcgc agtccgacgc cgccgacgag ccggtccatc cagccgcgaa ccgcccaggc
     3781 caacgggaac gagtaccagc cgcgctcgcc accgataccg gtgacgactg cccagagcgc
     3841 agcgggctcg gcgtcgctgt gctgttcgcg ctcgtcgaga taaacggttc cgccggacca
     3901 ccgcgggtcg ctggggaggg gatcggacgg catgtcgggc accgtcgcgc ccgaccagcg
     3961 ggtctcgact tccccaccgc tgatccgcgc caacgccagc tgaacggccc ggtcgtagcc
     4021 gatccggcct tccggcgggt ctggtacgta cccgaggatg tcctgctccg agcacaccac
     4081 ctcgtgtacg agcgactcga tgagcgggtc tgcgattccc ttcggcaccg gcgtcacgat
     4141 gttcacccag tgcgccgaca ggcgcggggt cagcacgggc accgggacga tgcgccgcca
     4201 tggcagcccg gccaccgcgg cgtatcgctg catcatgtcg aggtaggtca gcacatccgg
     4261 accgccgatg tcgaacgtcc gattgacctc ggacggcagc gtggccgcgc cgaggaggta
     4321 gcgcaggacg tcgcgtaccg cgatgggctg gatccggttg cgtacccagc gcggcgtcag
     4381 catgaccggt aggcgctcgg tgagatagcg caacatctcg aagctcgccg agccggaccc
     4441 gatgatcacc gccgcctgca gcaccgcggt cggcacgccg ctgcgcaaca ggatctcgcc
     4501 cacctcggtg cgggacgcga ggtgctcgga c
//
LOCUS       DASRSC010000079         3881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1171748,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000079 DASRSC010000000
VERSION     DASRSC010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1171748"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1262)
                     /locus_tag="VFE39_03890"
     CDS             complement(189..1262)
                     /locus_tag="VFE39_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HET6257772.1"
                     /translation="MQPDYPQPVPTGSTERGLPVDWDLARRTAGRLMQPGPEATRIEA
                     TELVARLRSDAAVAEGHVRDVTGLGHGLPLLPADVVDRPAWAAAAVLGMAALTADAQL
                     PAVSRAARALTARTAGLQVGGIVAYLGGRVLGQYDPFGGPAGDGRLLLVAPNVHAAQQ
                     ALDVPAADFGLWVCLHEATHRLQFTAVPWLRSYFAAEVGQFLSMAQGSDRSAVLDRLP
                     EVLRAVRSSRGDSLAIVELLQGPEQRAVLDRLLALTTLLEGHADHVMDAAGPQVVPSV
                     ATIRSRFTARRRGGGLVDRVLRALLGVEAKVRQYAVGSAFTRHVVLAVGMEGFNRVWE
                     SPESLPSRSELADPGTWLRRVHS"
     gene            complement(1369..2718)
                     /gene="dacB"
                     /locus_tag="VFE39_03895"
     CDS             complement(1369..2718)
                     /gene="dacB"
                     /locus_tag="VFE39_03895"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HET6257773.1"
                     /translation="MVAVAGASGGAVALTGPTLVQRLGLVQAAETVQAPPEPQAVLQP
                     LPADAPLPTTAGLTGALNGAAEAMPGTFTGIVVDPATGKALWERTPEVALVPGSAGKL
                     LTASAALLTLNPTDTFVTRVVQGSEPGTVVLVGGGDPTLTTLPEGQNSVYPNAPRLSD
                     LAAAVRKAAPRPVTRVLVDTSRYQGPTMAEGWDPTDVAAGNITPIEPLMVDGGRINPK
                     LQDGPRVPDPALAAGRALAQDLGADPSDVAVGAATPGSKVLGSVRSAPVAELIEQMLR
                     DSDNVLAEMFSREVAIARDGDPTFTGGVQQTMAALAQAGFDPSGAVMHDGSGLSTEDR
                     VPARLLGQLLGAAAAPTEKPGDIEFLRPIVSGLPVAGGYGTLEDRFGRGATSSTGRGV
                     VRAKTGTLTDVSSLAGIVTDVDGRLLVFALMSNGASPATIRPKLDAMAAQLSRCGCR"
     gene            2952..3455
                     /locus_tag="VFE39_03900"
     CDS             2952..3455
                     /locus_tag="VFE39_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET6257774.1"
                     /translation="MDFDVTIEIPKGGRNKYEVDHKTGRIRLDRTLFTATQYPADYGF
                     IEESLGEDGDPLDALVLVQEPTFPGCLIRSRAIGMFRMKDEKGGDDKVLCVPSDDPRQ
                     EHLRDIHHLPEFDRAEIQHFFEIYKTLEPGKSVEGATWVGRADAEREIKASWQRAKDA
                     EQSGAAH"
     gene            complement(3515..>3881)
                     /locus_tag="VFE39_03905"
     CDS             complement(3515..>3881)
                     /locus_tag="VFE39_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6257775.1"
                     /translation="ANEPILEAVQGGTEPSLEDSSFLSAADPVLSRPILDGFADSMSV
                     VFVAGSAVLVIGLFAVIMMKEVPLRTQSGVDARRAEELNAVETLAPSATPVAVPAEAC
                     GEQDGTGNGWPRKSSWPRE"
BASE COUNT          572 a         1402 c         1439 g          468 t
ORIGIN      
        1 gccgaggacc aggcggaacg gggacctcgc aggccgaaac ccggcgactc gcgggcggga
       61 acccggcgac tcgcaggccg aaacccggcg actcgcaggc cgaaacccgg cgactcgcgg
      121 gcgggaaccc ggcgactcgc aggcgggaac ccggcgactc gcgggccgaa accgggcgac
      181 tcgcggggtt agctgtggac gcggcggagc caggtgccgg ggtcggcgag ctcggagcgg
      241 ctcgggaggg actcggggct ttcccagacg cggttgaagc cctccatgcc cacggccagc
      301 accacgtgcc gggtgaaggc ggacccgacg gcgtactggc ggaccttcgc ctccaccccg
      361 agcagtgcgc gcagcacgcg gtcgacgagc cctccgccgc gacgccgtgc ggtgaaccgc
      421 gagcggatgg tggccacgct cggcaccacc tgcggcccgg cggcgtccat cacatggtcg
      481 gcgtggccct ccagcaacgt ggtgagggcc agcagccggt cgagcacggc ccgctgctcg
      541 ggaccctgga ggagctccac gatcgccagc gagtcgcccc gggacgaccg caccgcgcgc
      601 agcacctcgg ggagccggtc caggacggca ctgcggtcgc tcccctgcgc catggagagg
      661 aactggccga cctcggcggc gaagtaggag cgcagccacg gcacggcggt gaactggagc
      721 cggtgggtgg cctcgtgcag gcacacccag agtccgaagt cggcggccgg cacgtcgagc
      781 gcctgctgcg ctgcgtgcac gttcggcgcc accagtagca atcgcccgtc ccccgcggga
      841 ccgccgaagg ggtcgtactg gcccaggacc ctgcctccga gatacgcgac gatgccgccg
      901 acctgtagcc ccgcggtacg ggccgtcagc gcgcgggcgg cgcgggacac ggcgggcagc
      961 tgagcgtcgg cggtgagcgc cgccatgccg agcacggcgg cagcggccca ggccggccgg
     1021 tccacgacgt cggcgggcaa gagcggcagg ccgtgcccga ggccggtgac gtcgcggacg
     1081 tgcccctcgg cgacggccgc atccgagcgc agccgtgcga cgagctcggt tgcctcgatg
     1141 cgggtggcct cgggaccggg ttgcatgaga cggcccgcgg tgcgacgcgc caggtcccag
     1201 tcgaccggga ggccgcgctc ggtcgagccg gtcggaacgg gctgggggta atcgggctgg
     1261 atggcgtcgg tcgcgcgggg cacccgcccc acgctacgca acacgcggcg ggtgtgcccc
     1321 gctcggcgac gcccaccccg caccgcgcag agacccgcag cgggagcctc agcggcagcc
     1381 gcagcggctc agctgcgccg ccatggcgtc cagcttcggc cggatcgtgg caggggaagc
     1441 accgttcgac atcagcgcga agaccagcag ccgtccgtcg acgtcggtca cgatgccggc
     1501 gaggctgctg acgtcggtca gggtgccggt cttcgcacgt acgaccccgc ggccggttga
     1561 ggaggtcgcg ccgcgcccga agcggtcctc gagggtcccg tagcccccgg caacgggcag
     1621 gccgctgacg atggggcgca ggaactcgat gtcgcctggc ttctccgtcg gcgcggcggc
     1681 agcgccgagc agctgcccga gcaggcgggc gggcacgcgg tcctcggtgg acaggccgct
     1741 cccgtcgtgc atgacggcgc cgctcgggtc gaagccggcc tgcgcaagcg cggccatcgt
     1801 ctgctgcacg ccgccggtga acgtcgggtc gccgtcgcgg gcgatcgcga cctccctgga
     1861 gaacatctcg gcgagcacgt tgtccgagtc gcgcagcatc tgctcgatga gctccgcgac
     1921 gggagccgac cgcaccgagc cgagaacctt cgagcccggc gtagccgcgc ccactgcgac
     1981 gtcggagggg tcggcgccca ggtcctgcgc cagcgcgcgg cccgcagcca gcgcgggatc
     2041 ggggacgcgg ggcccgtcct gcagcttcgg gttgattcgg ccgccgtcca ccatgagcgg
     2101 ttcgatcggc gtgatgttgc cggcagccac gtccgtgggg tcccagccct cggccatcgt
     2161 cggtccctgg tagcggctgg tgtcgacgag cacgcgcgtg accggccgcg gtgccgcctt
     2221 ccgcaccgcg gcggcaaggt cggagagccg tggcgcgttg gggtacacgc tgttctggcc
     2281 ctcgggaaga gtggtgaggg tggggtcccc tccgcccacg agcaccaccg tgcccggttc
     2341 cgacccctgc acgacgcggg tgacgaaggt gtcggtgggg ttgagcgtga gcagcgcggc
     2401 ggaggcggtg agcagcttgc ctgcggagcc ggggacgagc gcgacctccg gagtgcgctc
     2461 ccagagcgcc tttccggtgg ccgggtcgac aacgatgccc gtgaacgtcc cgggcatcgc
     2521 ctcggcggcg ccgttcagcg ctccggtcag cccggcagtg gtcgggagtg gcgcgtcggc
     2581 cgggagcggc tggagcaccg cctgcggctc cgggggcgcc tggacggtct cggctgcctg
     2641 tacgagcccg agccgctgca ccagtgtcgg gccggtcagc gccacggcac cgccggacgc
     2701 gccggcgacg gcgaccacga cgaggacgat gaggccccgt cgcgcacggc gacccggcaa
     2761 acgcacagtg tcaccgatcc ggcgcccaat gcccacgtcg cccctccccc cgtcgccgcg
     2821 cacggccgca cacgtcggcc ggtcaacacc ctgccgacgc ggtcccccac actagaggca
     2881 ggcgccggag ccggtcgccc gaccggcatc ggctccgtac atgcccgttt ctgagcacca
     2941 ggagagcgct agtggacttc gacgtcacga tcgagatccc caagggcggc cgcaacaagt
     3001 acgaggtgga ccacaagaca gggcggatca ggctcgaccg gaccctgttc acagccaccc
     3061 agtacccggc cgactacggg ttcatcgagg agagcctggg cgaggacggc gacccgctcg
     3121 atgccctggt gctggtacag gagccgacgt tcccggggtg cctgatccgc tcccgcgcga
     3181 tcggcatgtt ccgcatgaag gacgagaagg gtggggacga caaggtcctc tgcgtcccga
     3241 gcgatgatcc gcgccaggag cacctgcgcg acatccacca cctgcccgag ttcgaccggg
     3301 cggagatcca gcacttcttc gagatctaca agaccctgga gccgggcaag agcgtcgagg
     3361 gcgccacctg ggtgggccgg gccgacgccg agcgcgagat caaagcgtcc tggcagcggg
     3421 cgaaggacgc cgagcagtcg ggcgccgcgc actagacggc agaacggctg ccgctcggcg
     3481 ggcgtcccgc gtctcgacga gccctgctcc gggctcattc cctgggccac gagctcttcc
     3541 gtggccaccc gttgcccgtc ccgtcctgct ccccgcaggc ctccgctggt acggcgacgg
     3601 gggtggccga tggcgccagc gtctccaccg cgttcagctc ctccgcgcgc ctggcatcga
     3661 cgcccgactg cgtgcgcagg ggtacctcct tcatcatgat cacggcgaag aggccgatca
     3721 cgagcaccgc cgaccccgca acgaacacca cgctcatgga gtccgcgaat ccgtcgagaa
     3781 tcggtcggct caggacgggg tcggctgccg acaggaacga ggagtcctcg agcgacggct
     3841 cggtgccacc ctgcacggcc tcgaggatcg gctcgttggc c
//
LOCUS       DASRSC010000080        15872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2810529,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000080 DASRSC010000000
VERSION     DASRSC010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 15872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15872
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2810529"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VFE39_03910"
     CDS             complement(<1..605)
                     /locus_tag="VFE39_03910"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET6257776.1"
                     /translation="MSDMGTEARPDGVRLQKVLAQAGVASRRAAEELIAAGRVSVDGK
                     IVREMGRRIDPETAVVHVDGARIVVRDDLVYLALNKPRGLLSTMSDDRGRPCVGDLIV
                     ERGMVDEHFDGAPRARLFHVGRLDADTEGLLLLTNDGDLGHRLMHPSYEIEKTYLAEV
                     VGQVSRDLGRRLREGIDLEDGPVSVHSFKLVDFNAGRSMVEL"
     gene            complement(630..1244)
                     /gene="scpB"
                     /locus_tag="VFE39_03915"
     CDS             complement(630..1244)
                     /gene="scpB"
                     /locus_tag="VFE39_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HET6257777.1"
                     /translation="MSDDRPVSLTDVTTGSDGPDPDQVELSDTELDAALEALLLVVDA
                     PTDEETLALAVEQPVARVRAALLRLSMSYAQAGRGIDLRHAGAGWRFYTRDAYAPYVE
                     RLLLDGQRARLTRAALETLAVVAYRQPVTRARVSAVRGVNCDGVLRTLLTRGLVQEAG
                     VDAATQGTLFRTTELFLERLGLSSLEDLPPLAPLLPDVDAIDEL"
     gene            complement(1237..2070)
                     /locus_tag="VFE39_03920"
     CDS             complement(1237..2070)
                     /locus_tag="VFE39_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET6257778.1"
                     /translation="MGGGPLLGPEARPGPFRVRLHNFEGPFDLLLQLIGKHELDITEM
                     ALHTVTDDFIAHLTALGDDADLDETTEFLVIAATLLDLKAARLLPDAEVEDAEDLALL
                     EARDLLFARLLQYRAYKQVAGLFVELEAGAMRRFPRSVALEERFAALLPEVLLGVDPA
                     TFADLAASVFRPKPPPTVGLDHLHFAKVSVAEHAALLRERLAELGVATFAALTADCTG
                     PLEVVARFLAVLELYREASVYLEQPEPFGELTVRWSPADQPGSHPVPGSDQAIPDDRD
                     E"
     gene            complement(2094..3011)
                     /locus_tag="VFE39_03925"
     CDS             complement(2094..3011)
                     /locus_tag="VFE39_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6257779.1"
                     /translation="MDTPRPFAPRTEPLDEDRYGDDHDGIEPDVAADSVGRPRVKVPE
                     PAPLTEHGPARVIAVANQKGGVGKTTSTINVGAALAEYGRKVLLVDFDPQGALSVGLG
                     VQAHELETTIYNLLMERGVEIEDVILETSVEGMDLLPSNIDLSAAEVQLVTEVGREQA
                     LGRAIKPVLDKYDIILIDCQPSLGLLTINALACADQILIPLACEFFSLRGVALLMDTI
                     DKVQERLNPDLQILGILATMFDPRTLHTREVRQRVIEAFGDLVFESVINRTVRFPETT
                     VAGEPITSWAPSSNGAEAYRLLAREVIAR"
     gene            complement(3208..3960)
                     /locus_tag="VFE39_03930"
     CDS             complement(3208..3960)
                     /locus_tag="VFE39_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET6257780.1"
                     /translation="MLAAQRRDLLLDRLRTEGRVVAKDLAGELGVSEDSVRRDLRELA
                     AAGLCQRVYGGALPVSPAIADHAGREHVAPGSKARVAATAAGLVAPGSTVIMDGGTTA
                     LAVVRALPLDLQATVVTHSPTVAAALVDHRDVEVFLLGGRLFRHSLVTCGAAAVEAAQ
                     AVTADVFLLGVTGVHHETGLTTGDADEAAMKRALARRAADTYVLASAEKIGAASRFAV
                     LPLVEVTGIVTDADLGRAAMRELAATGVNLIT"
     gene            4010..4396
                     /locus_tag="VFE39_03935"
     CDS             4010..4396
                     /locus_tag="VFE39_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HET6257781.1"
                     /translation="MTTPLLILIAGPYRSGTGDDPDLMAANLARLEEAAWPIFRAGHV
                     PMIGEWVALPVLRSAGATGVTDPLAEQVMYPTAQRLLQHCDAVLRLPGESTGADQDVT
                     IARERGIPVYTALEEIPGCGATAGAR"
     gene            complement(4421..5335)
                     /locus_tag="VFE39_03940"
     CDS             complement(4421..5335)
                     /locus_tag="VFE39_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET6257782.1"
                     /translation="MGARPADVVEAFLHHLTVERGSAVNTLRSYGADLRRYQEYLVAL
                     GIDDLAAVREADVAGFVAALRTGPVPLAASSAARALAAVRGLHRFAVRDGLVADDVAR
                     EVPPPALPVRLPRALTLDHVEQLLAGCVGDGPGPLRDRALLELLYSSGARISEAVGID
                     LDDLDVANRTVLLRGKGGKQRLVPVGRPALAAVDEYLVRARPGLAARGRGTPALLLNA
                     RGGRLSRQSAWHVLRGAAETAGLSAAVSPHTLRHCFTTHLLAGGADVRVVQELLGHAS
                     VTTTQVYTNVTVDTLREVYATSHPRALG"
     gene            complement(5427..6413)
                     /locus_tag="VFE39_03945"
     CDS             complement(5427..6413)
                     /locus_tag="VFE39_03945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET6257783.1"
                     /translation="MRFGMFVPQGWRHDLVGIDPRDHWTTMKGLAQHADAGPWESIWV
                     YDHFHTVPAPTDQATHEAWTLMSAFGAVTERVRLGQMCTCMSYRNPVYLAKVAATCDI
                     VSGGRIEMGIGAGWYEHEWRAYGYGFPPARERLRMLDEGVQIMRQAWTEGSASLDGKQ
                     YQVDEAIVRPLPLQDGGIPLWIAGGGEKVTLKIAAKYAQYTNFEGTLDGFTRKSELLK
                     GHCETVGTDFDAIVRSSNFNVAIGSTEADVERRLQDLKARLEPHVGAEQAEGVLGGFR
                     GGAACGTPEQIVEKLGALRDKGMTYGIFYFPEAAYDRSGIEQFEREVIPALR"
     gene            6635..7978
                     /locus_tag="VFE39_03950"
     CDS             6635..7978
                     /locus_tag="VFE39_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HET6257784.1"
                     /translation="MSAGQVGVDDYALVRVPHEERYSWWSVAVQRFGQVSALSQFLLG
                     STIGFGMSFWNAFWAFTLGAVILELVSIGVGIIGCREGLQTSLLTRWTGFGHAGSAVV
                     GVAIGISLIGWFGIQSGVSAEGLVSLIGVLPTWAWSLVFGLAVTFIVLWGFASMKWVA
                     YVTVPAFVVLVAWSIGGKLFGHDAGTLVSSPPPGPHLSLLEGTTLVAGGFIVGAVVTA
                     DMTRFNRSSADVIKQTVVGFTVGEYLIGMSGVLLAHAVRSDDVITIVTSSVGWVGTLV
                     IIMGTLKINDWNLYSSGLGVVNFVGTVFGKRVNRGVVTLTLGVVGSLLAAGGILDSFI
                     GFLSVLGVAFPPIAGIMVAEYFVVKQWRPDLDRSRAAGTLPETAPTWVPATLVIWVLA
                     ALIGHFVTWGLPSINSLIAAFVLYVVAGKLGLVRGVGVSRTLEGTDVPVADREVV"
     gene            7978..9549
                     /locus_tag="VFE39_03955"
     CDS             7978..9549
                     /locus_tag="VFE39_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HET6257785.1"
                     /translation="MRIGIDVGGTNTDAVLMDGDQVLAAIKAATTPDVTSGIVTALDG
                     LQGQHPFPPADVQAVMIGTTHFINALVEARGLAPTAALRLGLPATASLPPLVDWPDRL
                     VAAIAGRPYLAHGGHEFDGRPISPLDEDELRRHATDMAANGVRSVAISSVFSPVNTEF
                     EQRAAEVVAEILGPDVPVSLSHEIGRVGLLERENATIINAALRELAAQIVDGLSASIA
                     GHGITAPLYLSQNDGTLMDVEFSRRYPVATFASGPTNSMRGAAVLSGLATCAVVDVGG
                     TTTDVGVLTGGFPREATVEVSVAGVRTNFRMPDVLSIGLGGGSRVRGHGTDITVGPDS
                     VGYRLTEQAMVFGGDVLTATDVAVAAGRVAIGDPARVAGLSPSDVAAALERISTEVAD
                     IVDRMRISADPLPVVAVGGGSVLLPESFPGLGAVHRPEHFAVANAIGAAIAQVGGEVD
                     RVFAIEPGRRDAAVDEARQEAVDRAVVAGADPATVAIVDFDEVPIPYLPGNATRIRCK
                     AVGDLRIEHVAGRRA"
     gene            9546..10658
                     /locus_tag="VFE39_03960"
     CDS             9546..10658
                     /locus_tag="VFE39_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HET6257786.1"
                     /translation="MTGTLTAGAVSLPPLTSIGEDDLEALARGAAVLGTGGGGDPYIG
                     RLLAAQALRDHGPVELVAPAELPDDAQVFPVAMMGAPTVMVEKTPSVERIGYAVAALA
                     EHLGVTPTHIACIEAGGVNSTFPLVAAAQLGLPLVDGDGMGRAFPELQMVLPTLHGIA
                     ATPMSIGDEKGNVGLLTSVDNYWAESLARSLTITMGCAAIISNYAMRGAQARESLVAG
                     TLSMCTRIGRRVEQVRAEHGDAVGAVADEIGGRVLHTGKVVDVVRRTTTGFARGTAVV
                     SGAAGELLLEFQNEHLVALRDGVVQVTTPDLIVVLDTDSGEPVTTEALRFGHRVSVLA
                     APADERWHSPAGIALVGPRYFGYDTDPVRFDAGELP"
     gene            10655..11758
                     /locus_tag="VFE39_03965"
     CDS             10655..11758
                     /locus_tag="VFE39_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HET6257787.1"
                     /translation="MTWTLDPQDLPDLARGAALLGTGGGGDPHIGRLMVEAAIREHGP
                     VTVLDPDEVDDAAFVVPTAMMGAPTVMIEKIPRGDEAVLALRTLERHLGRSADATMPI
                     ECGGINSMIPLVVAATTGLPVVDADGMGRAFPELQMETFGVYGVPGSPMVVAGDHGET
                     VTIDTGTDNRRMEWLARGAAIRMGGAALIAEYAMSGAEVKRTAVPRTLSLGLSLGRAL
                     REAREQLRDPISALTEALAPTGYSHLRVLFAGKVADVERRTVDGFARGRARFVSFDGS
                     STLRTVFQNENLVAEVDGVVACIVPDLICVLEAESGEPITTETLRYGQRVVVLGISTP
                     EIMRTPEALAVFGPKCFGLDEEFRPVEDVLPVG"
     gene            11798..12871
                     /locus_tag="VFE39_03970"
     CDS             11798..12871
                     /locus_tag="VFE39_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HET6257788.1"
                     /translation="MTGARLGAADVDALVVGLRLLGSGGGGDPQPFRHALRRSLAGAD
                     VVLHDPADLGDAPVVAVGMLGATWVLTEKLPSGQEITTAVRAVARWTGIEPAAIMPYE
                     AAGLNGAMAVVAACELGLPLVDADLMGRALPRLNQLTRAVDGGPITPYAMAEPSGQIV
                     LVDNAAPAALERIARTVVAQGGGWVGGALAPVPASRASTDSCVGTLARALRLGRAHAG
                     LVRPTGGEVAAALGGRVLAAGRTVEIARHPSASFGRAGIAVACDGGGVLRIEAENEYL
                     LALLDGEPVASCPDLLCLLDRRTAAPIAVDGLRLGDDVLAVVLPGPQWWRADPHRLRH
                     VDPRAFGLDCDAVLLPDPVGLVA"
     gene            12868..14391
                     /locus_tag="VFE39_03975"
     CDS             12868..14391
                     /locus_tag="VFE39_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6257789.1"
                     /translation="MTGQGAWSGAGPAASAVALRALLAHPDWSGVTVLAGDGAGDGST
                     VSVREVRTVPDLRADPAGAPGALLAVALSGDREDWHLDALLRRAAAAGVVTVLLPGTE
                     PLRLASHLLADRVGLTVLGAADPLSVALAATRLLREPDQVVAELVTRTVAACAAAPGG
                     VDELVAELARVWRRPVWLLDGTGEVVAGPEPGPPGGQEMVTVLMPVGAQRPRPTALAA
                     AVPTGIPAELAAVRAALGVAAGAVGHRMAEKRLTVERDARLRMSLLAELVQAAAEPDP
                     GTRRRALDAGWQLEGWHVGMRIDVPGTVDPVTMRPEVLRAFDAAHLSALVVEQGSGWG
                     AWSTFAEEPAPDQLQRHAMAVRRAQWLLRSSLPSAMGVGRMHRGATGLARTLGEAGDA
                     ARLAGARSASGYFVHVDRLGLGQLLLAWTRTDTFLPAARSMLEPLRAQSGDLLTTLTA
                     YLDAESSFTETAAVLGVHRNTVTARVARAQELLGVELTDPDERLALHLACRSVLFHT"
     gene            14417..14932
                     /locus_tag="VFE39_03980"
     CDS             14417..14932
                     /locus_tag="VFE39_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HET6257790.1"
                     /translation="MSMPGSRPMRIGNAEREAAVRALGEHYAQGRLDSQEYEERTSAA
                     YAARSTDDLAPLFEDLPGHGEQATTVLPVPPTAAAPVPQAPPHDVAAPYGRDPATGLP
                     YSDRYKVVAGVLQLLLPFGIGRFYSGHTGIAIAQLLLSFIGIGVLWAFIDGIVILAGR
                     PTDPHGRPLRP"
     gene            complement(15006..15671)
                     /locus_tag="VFE39_03985"
     CDS             complement(15006..15671)
                     /locus_tag="VFE39_03985"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6257791.1"
                     /translation="MSAPRHDFPVRSSKVIYAGAVMALRADEVVMPGGRVAVREVLEH
                     PGAVAIAALDVDDRVMMIHQYRHPVRRRLWELPAGLLDIDGEDPLETAKRELAEEAGL
                     AATDWSTLVDVVPSPGFSDESVRVYLARGLSEVDRPDMGDDEEADLSVRWVSLSVAVR
                     MVLSGTIVNGVTVAGVLAAHALAGAPTAARPADAPWPDRPTRFAERKAEVADSPDRAD
                     PAT"
     gene            complement(15668..>15872)
                     /locus_tag="VFE39_03990"
     CDS             complement(15668..>15872)
                     /locus_tag="VFE39_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET6257792.1"
                     /translation="FVELPGDVHPFFVGTQAHPELKSRPTRPHPLFSAFVHAALAYRA
                     ADRLPVELPPRERAAVGVNGTAS"
BASE COUNT         2128 a         5751 c         5711 g         2282 t
ORIGIN      
        1 agctcgacca tcgaccgtcc ggcgttgaag tccacgagct tgaacgagtg caccgacacg
       61 ggcccgtcct ccaggtcgat cccctcgcgc agcctgcgtc cgaggtcgcg ggagacctgg
      121 ccgacgacct cggccaggta ggtcttctcg atctcgtacg acgggtgcat cagccggtgg
      181 ccgaggtcgc cgtcgttggt gagcagcagc agcccctcgg tatcggcatc gagccgcccg
      241 acgtggaaca gccgggcgcg cggggcaccg tcgaagtgct cgtcgaccat gccgcgctcc
      301 acgatgagat cgccgacaca cggcctgccg cggtcgtcgg acatcgtgga gagaagtccg
      361 cgtggcttgt tcagcgccag gtacacgagg tcgtcacgga ccacgatccg cgccccgtcc
      421 acgtggacga cggccgtctc cgggtcgatc cggcggccca tctcccgaac gatcttgccg
      481 tcgacgctca cccggcccgc cgcgatcagc tcctcggccg cacggcggga cgcgacaccg
      541 gcctgggcca gcaccttctg cagacgcact ccgtcgggcc gggcttcggt acccatgtca
      601 ctcatctcaa agctcattcg tcacactcgt cacaactcgt cgatcgcgtc cacatcgggc
      661 agcagcggcg ccagcggagg caggtcctcc agcgacgaca gccccaaccg ctcgaggaac
      721 agctccgtgg tgcggaacag ggtgccctgc gtggcggcgt cgacacccgc ctcctggacc
      781 agaccgcggg tgagcaacgt ccggagcaca ccgtcgcagt tgacaccccg gaccgccgac
      841 actcgggccc gcgtgacggg ctgccggtac gccaccacgg ccagcgtctc cagggcagcc
      901 cgcgtgagcc gggcccgctg gccgtcaagc agcaggcgct cgacgtaggg ggcgtacgcg
      961 tcacgggtgt agaagcgcca tcctgcaccg gcgtggcgca ggtcgatgcc ccgtccggcc
     1021 tgggcgtagc tcatcgacag gcgcagcagc gccgcgcgca cccgcgcgac gggctgctcc
     1081 acggccaggg ccagcgtctc ctcgtcggtc ggcgcgtcga cgaccaacaa cagcgcctcg
     1141 agggcggcat cgagctctgt atcgctcagc tcgacctggt cggggtcggg accgtccgat
     1201 ccggtggtca catcggtgag gctcacgggc ctgtcgtcac tcatcacgat cgtcggggat
     1261 ggcctggtcg ctgccgggca cgggatgcga tccgggctgg tcggccggac tccagcgtac
     1321 tgtcagctcg ccgaacggct cgggctgctc caggtacacg ctggcctcac ggtagagctc
     1381 cagcacggcg aggaaccggg cgaccacctc cagcggcccg gtgcagtcgg cggtgagcgc
     1441 cgcgaacgtg gccactccca gctccgcgag ccgctcgcgc agcagcgctg catgctcggc
     1501 caccgacacc ttggcgaaat gcaggtggtc gagcccgacc gtcggcgggg gtttgggccg
     1561 gaacaccgag gcggcgaggt cggcgaacgt cgccgggtcg acccccagca gcacctcggg
     1621 cagcagcgcg gcgaaccgct cctccagcgc gaccgaccgc gggaagcggc gcatcgcgcc
     1681 ggcctcgagc tcgacgaaca gccctgccac ctgcttgtac gcccggtact gcaggagccg
     1741 ggcgaacagc agatccctgg cctccagcag cgcgaggtcc tcggcgtcct cgacctcggc
     1801 gtcgggcagc agcctggccg ccttgaggtc gagcagggtg gccgcgatga ccaggaactc
     1861 cgtggtctcg tcgaggtcgg cgtcgtcgcc gagagccgtg aggtgcgcga tgaagtcgtc
     1921 ggtgacggtg tgcagcgcca tctcggtgat gtcgagctcg tgcttcccga tcagctgcag
     1981 cagcaggtcg aacgggccct cgaagttgtg caggcgcacc cggaagggcc ctggccttgc
     2041 ctcgggaccg agcagggggc cgccaccgat gggcgtgccc tcatcgacca gcgtcaccgg
     2101 gcgatgacct cgcgggccag cagccggtac gcctcggcac cgttcgacga gggagcccag
     2161 ctggtgatcg gctcgcctgc caccgtggtc tccgggaagc gcaccgtgcg gttgatcacc
     2221 gattcgaaca ccaggtcgcc gaatgcctcg atcacccgct ggcggacctc gcgggtgtgc
     2281 aacgtgcgcg ggtcgaacat cgtggccagg atgccgagga tctgcaggtc cgggttgagc
     2341 cgctcctgca ccttgtcgat cgtgtccatc agcagagcga cgccccgcag gctgaagaac
     2401 tcgcacgcca gcgggatcag gatctggtcg gcgcaggcca gcgcgttgat cgtgagcagg
     2461 ccgagcgacg gctggcagtc gatgaggatg atgtcgtact tgtccagcac cggcttgatc
     2521 gcgcggccga gcgcctgctc acgtccgacc tcggtgacga gctgcacctc ggccgcggac
     2581 aggtcgatgt tgctcggcag caggtccatg ccctcgacgg aggtctcgag gatcacgtcc
     2641 tcgatctcca cgccccgctc catgagcagg ttgtagatcg tggtctcgag ctcgtgagcc
     2701 tgcacaccca ggcccacgga cagcgcgccc tgcgggtcga agtcgacgag cagcaccttg
     2761 cggccgtact cggcgagcgc cgccccgacg ttgatcgtcg acgtcgtctt gccgacgccg
     2821 cccttctggt tcgcgaccgc gatgacccgc gcggggccat gctcggtcag cggggccggt
     2881 tcgggcacct tcacgcgcgg tctccccacg gagtcggcgg ccacgtccgg ctcgatgccg
     2941 tcgtgatcgt cgccatagcg atcctcgtcg agcggctccg tgcggggggc gaagggccgc
     3001 ggtgtgtcca tgcggactcc ggtctctggc gggcgacccc acaaagggtc gatccgggca
     3061 gactaggagg cccgatggac gacgggcaac gcgccccgcc gtatggcggc cgtcggatcg
     3121 accggatgtg gccgacgaac catcaccgta cccgcgatct gcggcacgcg aaggacgcgc
     3181 gctcggttcc tgcccgtcat cgtccgttca ggtgatcagg ttcactcccg tcgcggcgag
     3241 ctcgcgcatg gcggccctgc cgaggtcggc gtcggtgacg atccccgtga cctcgacgag
     3301 cgggagcacc gcgaaccggg aagccgcgcc gatcttctcg gcactggcaa gcacgtacgt
     3361 gtcggcggcc cgccgcgcga gcgcgcgctt catcgcggcc tcgtccgcgt cgccggtggt
     3421 aagtcccgtc tcgtggtgga cgccggtgac gccgagcagg aacacgtcgg cggtgacggc
     3481 ctgcgccgcc tccaccgccg cagccccaca cgtgaccagg gagtgccgga agaggcggcc
     3541 gccgagcagg aagacctcga cgtcgcggtg gtccacgagg gctgccgcca cggtcgggct
     3601 gtgggtcacc acggtggcct gcaggtcgag cggtagcgcc cggacgaccg ccagagccgt
     3661 cgtcccgccg tccatgatca cggtgctacc cggcgcgacg agcccggcag ccgtggccgc
     3721 gacccgcgcc ttgctccccg gcgccacatg ctcgcggccg gcgtggtcgg cgatcgcagg
     3781 cgagaccggc aacgcgcccc cgtagacccg ctgacacagg ccggctgcgg cgagctcgcg
     3841 caggtcgcgc cggacgctgt cctccgacac cccgagctcg cccgccaggt ccttagcgac
     3901 gacgcggccc tccgtgcgga ggcggtcgag gagcaggtcg cggcgctgtg ctgccagcat
     3961 gcacgttctc ccttgttttt tccgactttt gcacgttacc gtgccgcctg tgaccacacc
     4021 actgctgatc ctcatcgccg gtccctaccg ctccggcacc ggcgacgacc ccgacctgat
     4081 ggccgccaac ctcgctcgcc tggaggaggc ggcgtggccg atcttccgtg ccgggcacgt
     4141 gccgatgatc ggcgaatggg tcgcgctccc cgtgctgcgc agcgcgggcg ccaccggggt
     4201 caccgatccc ctcgcagagc aggtcatgta tccgacggca cagcggttgc tgcagcactg
     4261 cgacgcggtc ctgcgactcc ccggcgagtc gacgggcgcc gaccaggacg tcaccatcgc
     4321 ccgtgagcgc ggcatccccg tctacacggc gctcgaggag atacccggct gcggcgcgac
     4381 ggccggcgcc cgctgagccg gaccgtccgc aagcagcgcg ctacccgaga gctcgggggt
     4441 gggaggtcgc gtagacctcg cgcagcgtgt cgaccgtgac gttcgtataa acctgcgtgg
     4501 tggtcaccga cgcgtggccg agcagctcct gcaccacgcg gacgtcggcg cccccggcga
     4561 gcaggtgggt ggtgaagcag tgccgcaggg tgtgcggcga gaccgcggcg gacagtccgg
     4621 ccgtctcggc ggcgccgcgc agcacgtgcc acgcgctctg ccgggagagc cgcccgccgc
     4681 gcgcgttgag caggagcgcc ggggtaccgc ggccgcgggc ggcgaggcca ggacgggcac
     4741 ggacgaggta ctcgtccacg gcggcgagcg ccgggcgtcc cacgggcacg agccgctgct
     4801 tgccgccctt accgcgcagc agcaccgtgc ggttcgcgac gtcgaggtcg tcgaggtcga
     4861 tcccgacggc ctcggagatc cgcgccccgc tggagtagag gagctccaac agcgcacggt
     4921 cgcgcagcgg gccgggaccg tctccgacgc agccggcgag cagctgctcg acgtggtcga
     4981 gcgtcagtgc ccgcggcagc cgcaccggca acgccggcgg cggcacctcg cgggcgacgt
     5041 cgtcggccac cagcccgtcg cgcaccgcga agcggtgcag cccccgcacc gccgccagcg
     5101 cgcgggcggc cgacgacgcg gccagcggta cgggcccggt ccgcagagct gccacgaacc
     5161 ccgcgacgtc ggcctcccgc acggcagcga ggtcgtcgat cccgagggct accaggtact
     5221 cctggtagcg cctcaggtcg gcgccgtagg aacgcagcgt gttgacggcg ctcccccgct
     5281 ccaccgtcag gtggtgcagg aacgcctcca cgacatcagc aggccgcgct cccatggagc
     5341 catcctcccg cagccgccgc cccacgcccg gctccgccgc gcggtggatg ggacagcgcg
     5401 gtggatggat cagcgcggtg gatggatcag cggagcgccg ggatgacctc gcgctcgaac
     5461 tgctcgatcc cggagcggtc gtaggcggcc tccgggaagt agaagatccc gtaggtcatg
     5521 cccttgtcgc gcagcgcgcc gagcttctcg acgatctgct ccggcgtgcc gcacgccgcc
     5581 cctccgcgga acccgccgag cacgccctcc gcctgctccg cgcccacgtg gggttccagc
     5641 cgggccttca ggtcctgcag ccgccgctcg acatcggcct cggtgctgcc gatcgcgacg
     5701 ttgaagttcg acgagcggac gatggcgtcg aagtcggtac cgacggtctc gcagtgcccc
     5761 ttgagcagct cggacttccg tgtgaagccg tcgagcgtgc cctcgaagtt ggtgtactgc
     5821 gcatacttgg cagcgatctt gagcgtcacc ttctcaccgc cgcccgcgat ccacagcggg
     5881 atgcccccgt cctgcaacgg aaggggccgg acgatggcct cgtccacctg gtactgcttg
     5941 ccgtccagcg aggcgctgcc ctcggtccac gcctggcgca tgatctggac accctcgtcg
     6001 agcatgcgca agcgctcccg ggcgggcggg aagccgtagc cgtaggcccg ccactcgtgt
     6061 tcgtaccagc ccgcgccgat gcccatctcg atgcgcccgc cggagacgat gtcgcaggtg
     6121 gcagcgacct tcgcgaggta gacggggttg cggtagctca tgcaggtgca catctgcccg
     6181 agccgcaccc gctcggtgac ggcgccgaag gccgacatca gggtccacgc ctcgtgcgtg
     6241 gcctggtcgg tgggggcggg caccgtgtga aagtggtcgt agacccagat ggactcccac
     6301 gggcccgcgt cagcgtgctg cgcgaggccc ttcatggtgg tccagtggtc tcttggatcg
     6361 atgccgacga ggtcgtgtcg ccagccctgg ggaacgaaca ttccgaagcg aattccggtc
     6421 acgaacttcc atactagtcc catgcctcgc cagttcccca gtgtttcggc aacccggacc
     6481 gtgcactgtg cactgctgat ggccgttttg tggcgatctg cctagtgacg cgctcctgaa
     6541 atccgcttta cgtttcgcgc tgacgccgga gcaggttccg ccccgcgcgc cgagacgtca
     6601 cgaacggaca ggtcgagcgc agcgagggag agcgatgagt gctggacagg tgggcgtcga
     6661 cgactacgcg ctggtccgcg taccccacga agagcggtac tcctggtggt cggtcgccgt
     6721 gcagcggttc gggcaggtgt cggcgctgag ccagttcctg ctcggctcga cgatcggctt
     6781 cggcatgagc ttctggaacg ccttctgggc cttcacgctc ggcgccgtga tccttgagct
     6841 cgtctccatc ggggtcggga tcatcggctg ccgggagggt ctgcagacct ctctcctcac
     6901 ccgctggacc ggcttcggcc atgccggcag cgcggtggtc ggcgtcgcca tcggcatctc
     6961 cctgatcggc tggttcggca tccagtccgg cgtctccgcg gaaggcctgg tcagtctgat
     7021 cggggtcctg ccgacctggg cgtggtcgct ggtgttcggc ctcgcggtga cgttcatcgt
     7081 gctgtggggc ttcgcgtcga tgaaatgggt cgcctatgtg accgtgcccg ccttcgtggt
     7141 gctggtggcg tggtcgatcg gcggcaagct gttcggccac gacgcgggga cgctggtctc
     7201 gtcgccgccg ccaggcccac acctgtcgct gctggagggc accacgctcg tggcgggcgg
     7261 cttcatcgtg ggcgccgtgg tcaccgccga catgaccagg ttcaaccgca gctcggccga
     7321 cgtgatcaag cagaccgtcg tcgggttcac ggtcggtgaa tacctgatcg gcatgtccgg
     7381 ggtgctgctc gcccacgccg tccgctccga cgacgtcatc acgatcgtca cgtcgtccgt
     7441 cggctgggtg ggcaccctgg tgatcatcat gggcacgttg aagatcaacg actggaacct
     7501 ttacagctcg gggctcggcg tcgtgaactt cgtcggcacg gtgttcggca agcgggtcaa
     7561 ccggggggtc gtgacactca cgctcggcgt cgtcggcagc ctgctcgccg cgggcggcat
     7621 ccttgacagc ttcatcgggt tcctctcggt gctcggcgtg gcgttcccac cgatcgcggg
     7681 gatcatggtc gccgagtact tcgtggtgaa gcagtggcgt ccggacctgg accgctcccg
     7741 cgcggcggga acgctccccg agaccgcgcc cacctgggtg cccgccacgc tcgtgatctg
     7801 ggtcctcgcc gcgctgatcg ggcacttcgt cacctggggc ctgcccagca tcaactcgct
     7861 gatcgcggcg ttcgtgctct acgtggtggc cggcaagctc gggctggtgc gcggggtcgg
     7921 cgtgagccgc acgctggagg gcacggacgt gcccgtcgcg gaccgggagg tggtctgatg
     7981 cggatcggga tcgacgtcgg cggcaccaac accgacgccg tcctcatgga cggcgaccag
     8041 gtgctggccg cgatcaaagc cgcgacgacg ccggatgtga cgtcgggcat cgtcaccgcg
     8101 ctggacgggc tgcagggcca gcatccgttc ccgcccgccg acgtccaggc ggtgatgatc
     8161 ggcacgaccc acttcatcaa cgcgctggtg gaggcccggg ggctcgctcc gacggctgcg
     8221 ctgcgactcg ggctgcccgc caccgcgtcg ttgccgccgc tggtcgactg gcctgatcgc
     8281 ctggtcgcgg ccatcgccgg ccgcccctac ctcgcccatg gcggccacga gttcgacggc
     8341 aggcccatct caccgctgga cgaggacgag ctgcggcgcc acgccaccga catggccgcc
     8401 aacggcgtcc gctcggtggc gatctcgtcg gtgttctcgc ccgtgaacac ggagttcgag
     8461 cagcgcgcag cggaggtggt cgccgagata ctcggccccg acgtgccggt gtcgctctcg
     8521 cacgagatcg ggcgcgtcgg gctgctggag cgcgagaacg cgacgatcat caatgcagcc
     8581 ctgcgcgagc tcgccgcgca gatcgtcgac gggctgtcgg cgtcgatcgc gggtcacggc
     8641 atcaccgcgc cgctctacct cagccagaac gacggcacgc tgatggacgt cgagttctcc
     8701 cgccgctacc cggtggcgac cttcgcctcc ggccccacca actcgatgcg cggcgccgcg
     8761 gtcctgtcgg gcctcgccac ctgcgccgtc gtcgacgttg gcggcaccac cacggatgtc
     8821 ggtgtgctga ccggcggctt cccgcgcgag gcgaccgtcg aggtcagcgt ggccggggta
     8881 cgcacgaact tccgcatgcc ggacgtactg agcatcggcc tgggcggtgg cagtcgggtg
     8941 cgcgggcacg gcacggacat caccgtcggg cccgactccg tcggctaccg cctcaccgag
     9001 caggccatgg tgttcggcgg cgacgtactc acggccaccg acgtcgccgt ggccgccggg
     9061 cgcgtggcga tcggggatcc ggcccgcgtc gccggtctct cgccgtccga cgtggcggcg
     9121 gcgctggagc gcatcagcac cgaggtcgcc gacatcgtgg accggatgcg gatctccgcc
     9181 gacccgctgc cggtggtggc cgtcggcggc ggctcggtgc tgctacccga gtccttcccg
     9241 ggcctcgggg ccgtgcaccg gcccgagcac ttcgccgtgg ccaacgccat cggtgctgcg
     9301 atcgcccagg tgggaggtga ggtcgaccgc gtgttcgcga tcgaaccggg gcgccgcgac
     9361 gccgcggtgg acgaggcccg ccaggaagcg gtggaccgcg cggtggtcgc gggcgccgac
     9421 ccggccaccg tcgcgatcgt cgacttcgac gaggtgccca tcccctacct gcccggaaac
     9481 gcgacccgca tccgctgcaa ggccgtgggc gacctgcgga tcgagcacgt ggcggggcgc
     9541 cgcgcatgac cggcacgctg acggctggag cagtgtccct tccgccgctg acgtccatcg
     9601 gggaggacga cctcgaggcg ctcgcgcggg gagccgcggt gctcgggacc ggcggcgggg
     9661 gcgacccgta catcggacgg ctgctcgcgg cacaggccct ccgggaccac ggcccggtcg
     9721 agctcgtcgc gccggccgag ctgcccgacg acgcgcaggt gttccccgtc gcgatgatgg
     9781 gcgcgcccac ggtgatggtg gagaagacgc cgtcggtgga gcggatcgga tacgccgtcg
     9841 ccgcgctcgc cgagcacctg ggcgtgacgc ccacgcacat cgcgtgcatc gaggccggcg
     9901 gggtgaactc gacgttcccg ctggttgccg cggcgcagct gggcctcccc ctggtcgacg
     9961 gcgacggcat gggccgggcc ttccccgagc tgcagatggt gctgccgacc ctgcacggca
    10021 tcgcggccac gcccatgtcg atcggcgacg agaagggcaa cgtcgggctg ctcacctcgg
    10081 tcgacaacta ctgggccgag tcgctggccc gctccctgac gatcacgatg ggctgcgccg
    10141 cgatcatctc caactatgcg atgcgcggtg cccaggcgcg ggagagcctg gtggccggca
    10201 cgttgagcat gtgcacccgg atcgggcggc gcgtggagca ggtgcgcgcc gagcacggtg
    10261 acgccgtggg cgcggtggcg gacgagatcg gcgggcgcgt gctgcacacc ggcaaggtcg
    10321 tcgacgtcgt gcggcgcacc accaccggct tcgcccgcgg caccgcggtc gtctcgggcg
    10381 cggccgggga gctactgctg gagttccaga acgagcacct ggtggcgctg cgcgacggcg
    10441 tcgtccaggt cacgacgccg gacctgatcg tcgtgctcga cacagactcc ggggagcccg
    10501 tcaccaccga ggcgctgcgg ttcggccacc gggtgagcgt gctggccgca cccgccgacg
    10561 agcgctggca ctcccccgcc ggcatcgcac tggtcgggcc gcgctacttc ggctacgaca
    10621 ccgaccccgt ccgcttcgac gccggagagc tcccgtgacc tggacgctgg acccgcagga
    10681 cctgcccgac ctcgcccgcg gggccgccct actcggcacc ggcggcggtg gtgacccaca
    10741 tatcgggagg ctcatggtcg aggcggcgat ccgcgagcac gggccggtca ccgtgcttga
    10801 tcccgacgag gtggacgacg cagctttcgt cgtgcccacc gcgatgatgg gcgcgccgac
    10861 ggtcatgatc gagaagatcc cccggggcga cgaggcggta ctcgccctgc gcacattgga
    10921 gcgccacctc ggacgatccg ccgacgcgac gatgccgatc gagtgcggag gcatcaactc
    10981 gatgatcccg ctggtcgtgg cggccaccac cggtctgccc gtcgtcgacg ccgacgggat
    11041 gggccgggcg ttccccgagc tgcagatgga gacgttcggc gtctacggcg tgcccggctc
    11101 ccccatggtc gtggccgggg accacgggga gaccgtcacc atcgacacgg gcaccgacaa
    11161 ccgacggatg gagtggctgg cccgcggggc ggcgatccgg atgggcggcg cggcgctgat
    11221 cgccgagtac gcgatgtccg gcgcggaagt caagcgcacc gcggtgccac ggacgctctc
    11281 gcttggcctg tcgctcgggc gggcgctgcg ggaggcccgt gagcagctgc gcgacccgat
    11341 ctcggcgctc accgaggcgc tcgcccccac gggctactcg cacctgcgcg tgctcttcgc
    11401 gggcaaggtc gccgacgtcg agcgccgcac cgtcgacggg ttcgcccgcg gccgggcccg
    11461 gttcgtctcc ttcgacggca gctcgaccct gcgcaccgtc ttccagaacg agaacctcgt
    11521 cgccgaggtc gacggcgtcg tggcctgcat cgtccccgac ctgatctgcg tgctggaggc
    11581 cgagtccggc gagcccatca ccaccgagac gctgcggtac gggcagcgtg tcgtggtcct
    11641 cggcatctcc acgccggaga tcatgcgcac gccggaggcg ctggcggtgt tcggcccgaa
    11701 gtgcttcgga ctggacgagg agttccggcc ggtcgaggac gtactaccgg tcgggtgagc
    11761 acccgatcct gcgccgaggg tctgggacca tgcagaggtg acgggagcgc ggctgggagc
    11821 ggctgacgtc gacgcgctcg tcgtcgggct gagactcctc gggtcgggcg gcggtggcga
    11881 cccgcagccg tttcggcacg ccctgcgccg gtcgctggca ggcgccgacg tcgtgctgca
    11941 cgaccccgcc gatctcggcg acgcgcccgt ggtcgccgtc gggatgctcg gagccacctg
    12001 ggtgctcacc gagaagctac ccagcggcca ggagatcacc acggcggtac gggcggtcgc
    12061 gcggtggacc ggcatcgaac cggccgcgat catgccgtat gaggcggccg ggctgaacgg
    12121 cgcgatggcg gtggtcgcgg cgtgcgagct ggggctcccg ttggtggacg ccgacctgat
    12181 gggccgcgcg ctcccccgcc tgaaccagct cacccgcgcc gtcgacggcg gcccgatcac
    12241 cccgtacgcc atggccgagc cgagcgggca gatcgtgctg gtcgacaacg ccgcgccggc
    12301 ggcgctggag cggatcgcgc gcacggtcgt ggcccagggc ggggggtggg tcggaggcgc
    12361 gctcgcaccg gtgcctgcga gccgcgcgag cacggactcc tgtgtcggca ccctcgccag
    12421 ggcgctgcgg ctcgggcgcg cgcatgccgg gctcgtccgc ccgaccggcg gggaggtggc
    12481 cgcggcgctg ggcggccggg tgctcgcggc cggacggacc gtggagattg cccggcatcc
    12541 gtcggcgtcg ttcgggcgcg ccggcattgc cgttgcctgc gacgggggcg gggtgctgcg
    12601 gatcgaggcc gagaacgagt acctgctcgc cctgcttgac ggcgagccgg tcgcgagctg
    12661 ccccgacctg ctgtgtctgc tcgaccgccg cacggccgct ccgatcgcgg tggacggcct
    12721 gcgcctcggt gacgacgtgc tggccgtggt gttgcccggc ccgcagtggt ggcgcgccga
    12781 cccgcaccgg ctgcggcacg tcgacccgcg cgcgttcggg ctggactgcg acgcggtgct
    12841 gctgcccgac ccggtcgggc tcgtcgcgtg acgggacagg gagcctggtc cggcgcagga
    12901 ccggccgcgt cggcggtggc gctgcgcgcg ctgctggccc accccgactg gtccggggtc
    12961 accgtgctcg ccggcgacgg ggcgggcgac ggatccacgg tctcggtccg cgaggtgcgc
    13021 accgtgcccg atctgcgcgc cgatcccgcg ggcgctccgg gcgcgctgct cgccgtcgcg
    13081 ctgtcgggcg accgcgagga ctggcacctc gatgcgctcc tgcggcgggc cgcggccgca
    13141 ggcgtcgtga cggtgctgct gcccggtacg gagccgctgc gactcgcgag ccacctgctc
    13201 gccgaccggg tcgggctcac ggtgctgggg gctgccgacc cgctctcggt cgcgctggcc
    13261 gccacgcgcc tgctccgcga acccgaccag gtggtggcgg agctggtgac gcgcaccgtc
    13321 gccgcctgcg cagcggcgcc cggcggagtc gacgagctcg tggccgagct ggcgagggtg
    13381 tggcgccgcc cggtgtggct gctcgacggc accggcgagg tggtggcagg gccggagccc
    13441 ggtccacccg gcgggcagga gatggtcacc gtcctgatgc ccgtcggtgc gcagcggcca
    13501 cggcccaccg ccctcgccgc ggccgttccg acggggatcc cggccgagct cgcggccgtc
    13561 cgcgcggcac tgggggtcgc ggctggcgcc gtcgggcacc gcatggccga gaagcggctg
    13621 accgtggagc gtgacgcccg gctgcgcatg tccctgctcg cggagctcgt ccaggcagcg
    13681 gccgaaccgg atcccggcac ccggcgccgg gccctcgacg ctggctggca gctcgaggga
    13741 tggcacgtgg ggatgcgcat cgacgtcccc ggcactgtcg acccggtgac aatgcggccc
    13801 gaggtgctgc gcgccttcga cgccgcccac ctgagtgcgc tcgtcgtcga gcagggatcc
    13861 ggctggggcg cgtggtccac gttcgccgaa gagccggcgc ccgaccaact gcaacggcac
    13921 gcgatggccg tccggagagc acagtggctg ctgcgctcgt cgctgcccag cgccatgggt
    13981 gtcgggcgga tgcaccgcgg cgccaccggg ctggcccgca ccctcggcga ggccggcgac
    14041 gccgctcggt tggccggcgc acggtcggcc agcgggtatt tcgtgcacgt ggaccggctc
    14101 gggctcggcc agctgttgct cgcctggacc cggaccgaca cgttcctgcc tgccgcgcgg
    14161 tcgatgttgg agccgctgcg cgcgcagtcc ggcgacctgc tcaccacact cacggcctac
    14221 ctcgacgcgg agtcgtcgtt cacggagacc gccgcggtgc tcggggtcca ccgcaacacg
    14281 gtcaccgccc gcgtcgcgcg cgcgcaggag ctcctcggcg tggagctcac cgaccctgac
    14341 gagcggctcg cgctccacct cgcctgccgc agcgtgctgt tccacacctg accggcccac
    14401 cggtaccgtc ggcgccatga gcatgcccgg ctcccgtccg atgcggatcg gcaacgcgga
    14461 gcgggaggct gccgtgcgcg ccctcggcga gcactacgcg cagggccggc tcgactcaca
    14521 ggagtacgag gagcgcacat ccgcggcgta cgccgcccgc agcacggacg acctcgcgcc
    14581 cctgttcgaa gacctccccg gccacggcga gcaggccacg accgtgctgc cggttccgcc
    14641 gaccgcggcc gcgccggtgc cacaggcccc gccgcacgac gtcgccgcgc cgtatggtcg
    14701 cgacccggcg accgggctgc cctactccga ccggtacaag gtggtggccg gcgtgctgca
    14761 gctgctgctg ccgttcggca tcgggcggtt ctacagcggc cacaccggca tcgcgatcgc
    14821 ccagctgctg ctgagcttca tcggaatcgg ggtgctgtgg gctttcatcg acggcatcgt
    14881 gatccttgcc ggccggccca ccgaccccca cgggcggccc cttcgcccat gatcagcagc
    14941 gcagcgccgg gatgaccggc gctcgtcgtg cccgctcggc ggggccaacg gaggcatcct
    15001 tcccgttacg tcgcgggatc agcacgatcc ggactatcgg ccacttcggc cttgcgctcg
    15061 gcgaaccgcg tcggtcggtc cggccacggt gcgtcagccg gccgggccgc ggtgggcgcc
    15121 cctgccaggg cgtgggccgc cagcacgccc gccaccgtca cgccgttgac gatcgtgccg
    15181 gagagcacca tccgcaccgc gacggagagc gacacccacc ggacggacag gtcggcctcc
    15241 tcgtcgtccc ccatgtccgg ccggtccacc tcgctcagcc cccgcgcgag gtacacacgc
    15301 accgactcgt cggagaaccc cggcgagggc accacgtcga ccagcgtcga ccagtcggtg
    15361 gcagcgagtc cggcctcctc cgccaactca cgcttggccg tctcgagcgg atcctccccg
    15421 tcgatgtcga gcaggccggc cggcaactcc cagagccggc gccgcaccgg gtgccggtac
    15481 tggtggatca tcatcacgcg gtcgtcgacg tccagggcgg cgatcgcgac ggcgcccgga
    15541 tgctccagga cctcccggac ggcgacgcgt ccgcccggca tcaccacctc gtccgcgcgc
    15601 agggccatca ccgcgcccgc gtagatgacc ttgctggagc gcacggggaa gtcgtgccgc
    15661 ggggcgctca cgacgcggtc ccgttgacgc ctaccgccgc acgttccctg gggggcagct
    15721 cgaccgggag ccggtccgcg gctcggtagg cgagggcagc gtgcacgaac gcgctgaaca
    15781 gcgggtgcgg ccgcgtcggg cggctcttga gctccgggtg ggcctgggta ccgacgaaga
    15841 acgggtgcac gtcgcccggc agctccacga ac
//
LOCUS       DASRSC010000081        19555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2482431,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000081 DASRSC010000000
VERSION     DASRSC010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 19555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19555
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2482431"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..518)
                     /locus_tag="VFE39_03995"
     CDS             complement(258..518)
                     /locus_tag="VFE39_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257793.1"
                     /translation="MASIEEVRAGIALANDKASESLGALQQAHSSLEQAQGALLRATE
                     GSGQADVSEANGLLAQAVGSISEVQQAVHAAIQASEGVANRL"
     gene            696..2021
                     /locus_tag="VFE39_04000"
     CDS             696..2021
                     /locus_tag="VFE39_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET6257794.1"
                     /translation="MTSDIATKTPPTGFGAKVRQVGPGLLAAATGVGAGDLVATMVAG
                     ARFGTVLLWAAVLGAGLKLALGEGVGRWNLASGSTLLDGWRRLGYWATVFFGIYIVIW
                     GFVYGATAMSAVGLPLNALFGGLSVRYWAMIAGVVGLALVWMQRYHIFEKFMTVLVLI
                     KFVSVVSIAFLVSPDLGALANGLVPRLPEGSVIYVLGLIGGVGGTITMAAYGYWMIAK
                     GWKGTGWLSVMRLDNAVGYIMTAIFVIAMLIVGAEILLGQDLTESDTGLLTLGTELGA
                     RYGDWARILFLVGFLSVTTTSLLGVWNGVSLLFTDWTRTIRLPHGAKAELDFDTEALR
                     TGPATATVTGYEARVAERSLPFRAYLLWLTLPPMALLFLDRPFALTLVYGVLGAAFMP
                     FLGVTLIMLLNSRRIAREGRSGWLSNALLTAASALFFVLLVTDLINRFG"
     gene            2244..3422
                     /locus_tag="VFE39_04005"
     CDS             2244..3422
                     /locus_tag="VFE39_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HET6257795.1"
                     /translation="MPGFAGNRCIAGVPAVAALAVAAAIALWPAPAPATAPDLVISVS
                     RSSCGQDWTDPTPGPQTFQVHNTGAVTTTVDLVDPATGGIFGEIEGLGSGTTRSMPVT
                     LGDGEYAFRCLPDDADADVGPTVLFSGSGVRSSPAVVPVTRNDLLGPAKDYREKVITG
                     LDALVRDTGALATTVHHGDRQASEAAWLTAHLSYQQLGAAYGAFGDAASAIDGTADGL
                     AGGTSDPAFTGFHRLEFGLWHRAPMAALAAVADRLDADARALRQSFPAAQIDPNDLAL
                     RAHEIMEDALQFEVNGRADYGSGTTLATTLANIDGTRTALNVLRPLLQQRLPTLPTVD
                     TWLDRATSAVQAAWRPDGTWTPVGALDQRHREEIDGAVAELTEQLAPVATIVAPRRVS
                     "
     gene            3419..4642
                     /locus_tag="VFE39_04010"
     CDS             3419..4642
                     /locus_tag="VFE39_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HET6257796.1"
                     /translation="MTARLGRRSFLRRALVGAGVTAAAVAGGGVATAADGDSDAPAIP
                     FHGPHQAAILRPPAARSIVAAFDIIAADRADLTDLLRTVTDRARFLAAGGEPPALGIT
                     APPRDSGVLGPVVPSGDLAVIVGVGVSLFDDRFGLAGSRPARLTTMPTFPNDALDPAQ
                     CHGDLSLTLSASSPDTVVHALRDIARATRGGMQLRWRVNGFGSPPRPAGTPRNLMGFK
                     DGIANPDVDDAPEMDRLVWVGSGQSDEPAWTGGGSYQVIRVIRMFVEFWDRVSLSEQE
                     NVIGRRRDTGAPLDGTAEGDIPQFADDPVGTVIPLTSHIRRANPRTQQTDASRILRRA
                     TNYDRGIDSNGNLDMGLVFMCYQQDLRRQFEAVQERLTDEPLVDYISPFGGGYFFALP
                     GVTGPGDHYGRALLS"
     gene            4694..6337
                     /locus_tag="VFE39_04015"
     CDS             4694..6337
                     /locus_tag="VFE39_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6257797.1"
                     /translation="MGTKSSRWRRGRSLLGACALASAAVLAAVTVSTANAADTPPTAG
                     KHADQFNTATSIKHVVVIFGENVSFDHYFGTYPNAANTDGTPFTAAKQTPKIDGLTPD
                     LLQRNPNAYNPQRLSPDQALTCSQNHGYGAEQAAFDGGKMDQFVEKTETDTCSGQPIV
                     FGQPGLVMDYYDGNTVTAMWNYAQNYAMSDNSYNTTFGPSTPGAINLISGQTHGTQAV
                     TSVTHDPTSDPFVTSPDANGVGTVIGDPDPAYDDCSDKNHTATDNLASQSGKNIGDLL
                     NEKGVTWGWFQGGFRPTGNANGFAVCGQSHQNIGGISVTDYSPHHEPFQYYESTANSK
                     HLPPTSVAAIGQTDQANHQYDTADFDAALAAGNTPAVSFLKAPAYEDGHAGNSSPIDE
                     QRFVVDEINKIQQSKDWKSTAIVLAYDDSDGWYDHANAIVNGSQDTTQDQSICSGTPV
                     RLGGYADRCGYGPRLPLEVISPFSKVNFVDHTRTDQTSVLKFIEDNWSTGRIGDSSYD
                     ASAGDLTSMLDFKGAKARAVTLDPGTGAVVDGKPGKGNG"
     gene            6515..7378
                     /locus_tag="VFE39_04020"
     CDS             6515..7378
                     /locus_tag="VFE39_04020"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HET6257798.1"
                     /translation="MANTARTVDRVEDGLHAGPDAQVDDKRLLILPTDPRRTDPFLVL
                     SEDWFSTPGFEWHPHRGLETVTFVLDGVLEHGDNIGNAGALESGDVQWMTAGRGIIHR
                     ELAYRNERAHTLQLWLNLPAGARMVDTRYQDVLAAGRPRITRPGAVIDVVSGTVDEVT
                     GPALNHWPVQGVIVTIEPGVVVDLPVPAPDRAFALVLHGSTAVAGHQVNAGQVAWSEP
                     VTGPGSVLKLRAAPDTDDVARLMVYSGPPVAEPIVMGGSFVMNHAHEIEQARRDFRSG
                     GFGPVPRLARL"
     gene            complement(7388..7843)
                     /locus_tag="VFE39_04025"
     CDS             complement(7388..7843)
                     /locus_tag="VFE39_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257799.1"
                     /translation="MASTSAALAVVCLAAGLLHLVRLARLRRDVVRRGLVHGRALGMA
                     AMFSPLGDPVPTAVWPAVFVLAAVGLVAAPAMLLAGHVAWHALRWADRCRTTCPDAHP
                     AVSVQDGRDGPNAAVALRAPAFSACAPQTAAVSHVVLAAAMTAMLLRTP"
     gene            8057..8908
                     /locus_tag="VFE39_04030"
     CDS             8057..8908
                     /locus_tag="VFE39_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET6257800.1"
                     /translation="MAGESRVVRAALVQAKWTGDTASMVEVHESYARAAAEQGAQVMG
                     FQEVFNAPYFCQVQEAEHYRWAEPVPDGPTVQRMQALARETGMVLVVPVFELEASGFY
                     YNTAAVIDADGTFLGKYRKHHIPQVTGFWEKFYFRPGNLGWPVFDTAVGKVGVYICYD
                     RHFPEGWRALGLAGAQIVYNPSATSRGLSSYLWKLEQPAAAVANEYFIAAINRVGVEE
                     YGDNDFYGTSYFVDPRGQFVGEPASGSDEELVVRDLDLGMIDEVRQQWAFYRDRRPDA
                     YGPLVEQ"
     gene            8994..10298
                     /locus_tag="VFE39_04035"
     CDS             8994..10298
                     /locus_tag="VFE39_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020632850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HET6257801.1"
                     /translation="MSAQELFARTRAVLPSWLAVYYEEPIEIERGEGRYVWDADGKRY
                     LDFFGGILTTMTAHALPEVTKAVSEQAGRIIHSSTLYLNRPMVELAEQIAAVSAIPDP
                     KVFLTTSGTEANDAALLLASTLRRSNQILALRNSYHGRSFSTIAITGNRSWSPTSLSP
                     FQTYYVHGGQQYRSPYAGLSDGEYIAACVADLQDVLDQSGGDIAAFIAEPIQGVGGFT
                     SGPDGLLGAFAAVLRDRGILWISDEVQTGWGRTGDHFWGWQAHTADHGDATPDIVTFA
                     KGVGNGLSMGGVIARAEIMDCLGANSISTFGGSPLTAAGALANLRHLLDHDLQTNAAR
                     HGPALLDGLRALESPIVGDVRGKGLMIGIELVRPDTGSPGEPAPELAAAVLEATKRRG
                     LLVGKGGLHGNVLRIAPPLSLTADEAAEGLAALVASIQEVTP"
     gene            10295..11689
                     /gene="hydA"
                     /locus_tag="VFE39_04040"
     CDS             10295..11689
                     /gene="hydA"
                     /locus_tag="VFE39_04040"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HET6257802.1"
                     /translation="MTRTLIRNGLVITASDELATDVLIEDETVVALADPSISEGWTAD
                     TVIDATGKYVIPGGVDAHTHMELPFGGTFASDTFETGTRAAAWGGTTTIVDFAVQSMG
                     GSLREGLDAWHAKADGQCAVDYAFHMIMADVNEGSLKEMETLVGEGITSFKLFMAYPG
                     VFYSDDGKILRALQKGAETGGLIMMHAENGIAIDVLVEQALARGNTDPRYHGVVRHAL
                     LEAEATSRAIKLAQVAGAPIYIVHLSAIEALAELTRARDEGVNAFAETCPQYLYLSTD
                     DLARPGFEGAKYVCSTPLRPTEHQAALWRGLRTDDLSVVSTDHCPFCFKGQKELGLGD
                     FSKIPNGLPGVENRMDLLHQAVLDGHIGRRRWIEIACATPAKMFGLYPRKGTIAPGAD
                     ADVVIYDPAAQQVISVETHHMNVDYSCYEGRTVTGRAETVLSRGRVVVDKGEYLGAPG
                     HGRFLRRDTCQYLR"
     gene            11700..12701
                     /locus_tag="VFE39_04045"
     CDS             11700..12701
                     /locus_tag="VFE39_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HET6257803.1"
                     /translation="MDIGLVLQTDPPASTVVDLMVRAEELGFSHGWTFDSHVLWQEPY
                     VIYSRILERTSRLLVGPMVTNPGTRDWTVIASLHATLNDMFGNRTICGIGRGDSAMRV
                     QGRPPTTLARLEASMRVIRELAEGRAVDLDGTTVQIPWVRAGASLPVWMAGYGPKALD
                     LVGRAADGFILQLADPYLVEWTVKTVRAAAEAAGRDPAAITICVAAPAYVGDDLAHAR
                     DQCRWFGGMVGNHVADLVSRYGESSDAVPDVLTGYIAGRHGYDYSHHGRADNRSTDFV
                     PDEVVERFCLLGPPAAQLDKLAQLRELGVDQFAIYAMHDAREETIEAYGRSVIPALG"
     gene            complement(12789..15458)
                     /locus_tag="VFE39_04050"
     CDS             complement(12789..15458)
                     /locus_tag="VFE39_04050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6257804.1"
                     /translation="MGQTVPDVDMREAPPVRSKREIYTVMSGLMIAMLLAMLDNLIVG
                     TAMPTIVGDLGGLEHLSWVVTAYALTTAVSTPVWAKLGDLYGRKGIFMASIVVFLVGS
                     ALSGLSQNMMELIVFRAIQGIGAGGLMVGATAIIGDLVPPRDRGRYQGLMAAVMPLAF
                     IGGPLLGGFLTDNLSWRWAFYINLPLGVVALIVVWFTMHLPRVRREAKIDWLGAGTLA
                     VAISALTLLTSWGGSQYPWGSWQIVGLGVLTVVGTALFVRIERRVSEPILSLELFRNR
                     NFSAVTVVTFLTGFAMFGAVTYLPQYQQIVQGASATSSGLLLLPLMGGMLVTSLLSGQ
                     LVSRTGRYKVVIITGSLLMAAGLGLLSLMGLATTQLTTSLFMVVLGVGMGLLMQTTML
                     VMQNSVAQRDIGASSGASTLFRTVGGSLGVSLLGVFYTTRLTATLSDRLGTAGGEQVT
                     GGQITPAMLGGLPAAVRDAYQAAVASGTTTAFMWAGIVGLVAVLAALIIREVPLRGSA
                     PEAAGTMGGAVPAHPGQPTGPAPVLVLAGAGAGKDAGRHTLSTQESGVQLSGSNGNGR
                     SNGNGRSNGNGRSNGNGTPVAGAIGSIVTAPMSGRVDASSYRDGPGVFGIVSRGDGGR
                     LAQVVVTVTDPAGRQEARTSTAADGSYRVALPSGGMYLVVVATGTYQPHAGLVAVADR
                     PSRHDVSLTGTSSVFGVVQTPDPAGGLRSVGGANVTLIDAQGDVTAAAMAEPDGRYRL
                     AGVPDGIYTLTAASPGRQPVAVSVRLDIGAAIKRDIELPQRSRLLGTVTTASTGHGVP
                     EAMATLVDSTGTVVASTVTGPDGTFALDDLTEGTYTLTTTGYAPATRVVHVTAGARAA
                     VTVALGAPTTGPASSSPRTTAPEAAPLDVVGIR"
     gene            15561..16133
                     /locus_tag="VFE39_04055"
     CDS             15561..16133
                     /locus_tag="VFE39_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6257805.1"
                     /translation="MSSSTDERRTNTREQIRAVALELFSERGYDKTSLREIAERLGVT
                     KAAVYYHYRTKEEILSSLLDEHLAGMDAIVEWGQAQPPGPDRRREILQRYSALLAGPT
                     DEAPQLTRFLQESQTSIKELAAGAGMRERFQQLSALLVEPDAELVEQLRGRLALVALH
                     FGAFALDEIPGTRLERRDAALQVALELGLP"
     gene            complement(16266..17819)
                     /locus_tag="VFE39_04060"
     CDS             complement(16266..17819)
                     /locus_tag="VFE39_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HET6257806.1"
                     /translation="MTRLSRRGFLGLLGAAGAGAALAGCGLGSAPGQTGEQLTSAVPL
                     PDAFRVPLPVSPVARPVGTDPTGADLYRVTQRVANVEILPGLRTPVLGYDGIFPGPTF
                     ETRRGRLVVVQHRNELPVPTVVHLHGGHTPAESDGWPLDLVLPAGNAAGWTPHHGMLG
                     DVAAGEREYRYPTDQRAATLWYHDHRMDFTGPSVYRGLAGFHLVRDDIEDALPLPRGE
                     RELPLMICDRAFAADGSFAYPGLDQGMRSLPGVEDDWMEGVLGDVVLVNGAPWPVHEV
                     DASRYRLRLLNASNARRYRLVLRVPGVPDLPFVQIGSDNGLLAAPVTHQALEIAAGER
                     FDVVVDFTALPAGSEVTMINPLGTGRTRDVMRFRVVRTAPDDSRVPDRLADVEPLVPP
                     AGAPQRTFRFTRGSVGDHRGWTINGGSFDPARSLADITLGETEVWRFVSDVHHPVHVH
                     LDAFQVLRRGSEGPGPYDIGWKDTVDIRPAEVVDVAVRFEHYAGRFVFHCHNLEHEDM
                     AMMATIRTV"
     gene            complement(17816..18238)
                     /locus_tag="VFE39_04065"
     CDS             complement(17816..18238)
                     /locus_tag="VFE39_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257807.1"
                     /translation="MDTLAPTAPVARALRISLWLLRSLITVHLLAVLAQPVLAGLYLT
                     GDVDAIGVHGLVGTLLALVGLVVIGAALAYVLGGRGRLWVLPVAVLLVLAEGFQIAAG
                     YTRQLELHIPLGVAIVVTSVLLAIWVWTPSAALPRTRR"
     gene            18338..18685
                     /locus_tag="VFE39_04070"
     CDS             18338..18685
                     /locus_tag="VFE39_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6257808.1"
                     /translation="MRPDAVRGHLDGLILAVLEDGPRHGYAIIEALQTRSGGALELPT
                     GTVYPALRRLERAALLSGTWSTVGGRERRTYTLTRAGRHALANQRADWSEFSAVVDAV
                     LRPAAGRPAAGMP"
     gene            18682..19365
                     /locus_tag="VFE39_04075"
     CDS             18682..19365
                     /locus_tag="VFE39_04075"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HET6257809.1"
                     /translation="MTVTDPVDRHLAELERRLSGPSRTRRSMLREARDGLVDAVAAHR
                     RCGLDAHEAAAKAVRDFGSVREIAPLYQDELVARQGRWAALLLLVVFPGLTLGWGMLW
                     RLGVRWESEPASDLVVALARLQDTASAVVAVVALVLLAVTFRRAAPARQVAVLVSLTG
                     VVGAVVCGGTGLVMNLANGSAGRELVEANPYAVPAFGCSALLCALVLRSAVRTLLVAG
                     SPVPTAAER"
     gene            19427..>19555
                     /locus_tag="VFE39_04080"
     CDS             19427..>19555
                     /locus_tag="VFE39_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010306552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HET6257810.1"
                     /translation="MTAQNNPLTDRVALVAGATRGAGRGIAVELGAAGATVYVTGRS"
BASE COUNT         3043 a         6928 c         6789 g         2795 t
ORIGIN      
        1 cgtggtccgc cgcgcgcagc accgccaggc cggcgtccca ggcgagagcg cctgcggcgt
       61 cgccggcatg ccggccgcgc agcgccaggc tgatcggcat acccatgacg tgggcgacgt
      121 accggcgggt cggcgcaccg acggcggtgg tcaccgctct ccctcgctgc gctcggtcgg
      181 cgcttcgcga gcgacaccga aagctcactc accggtcatc gtcccggtca ccgaacgtcg
      241 ccacgctcac cggatcgtca cagccggttc gcgacgccct cggaggcctg gatcgctgcg
      301 tggacggcct gctgcacctc gctgatgctg ccgacggcct gggccagcag cccgttggcc
      361 tccgacacgt cggcctgccc gctcccctcg gtggcccgca gcagcgcacc ctgggcctgc
      421 tcaagagagg agtgcgcctg ctgcagtgcg ccgaggctct ccgaggcctt gtcgttggcc
      481 agcgcgatgc cggcccgaac ctcttcgatg ctcgccatga ccgaactccc cgtcgtgccc
      541 gtgctggctg gacaagggga aatctagtcg cggggccgct accggccgcg tgcgggccgt
      601 taccgatctt tccgctggcc gcgacgagtg ccgagcccta cgttcttccc aaaccttccg
      661 gatcagactt ccagcacgat gaggagcggg ggcgcatgac atccgatatc gctacgaaga
      721 caccaccaac cgggttcggg gccaaggtca ggcaggtggg gccaggtctg ctggctgcag
      781 ccaccggcgt cggcgcgggg gacctggtgg ccacgatggt ggccggggcc cgcttcggca
      841 cggtgctgct ctgggccgcc gtcctcggtg cgggcctgaa gctcgccctc ggggagggcg
      901 tgggccgctg gaacctcgcg tcgggctcca ccctgctcga cggctggcgg cgactgggct
      961 actgggccac cgtcttcttc ggcatctaca tcgtgatctg ggggttcgtc tacggggcaa
     1021 cggcgatgtc cgcagtcggg ctgccactga acgcgctgtt cggcgggctg tcggtgcggt
     1081 actgggcgat gatcgccggc gtcgtcggcc tggcgctggt gtggatgcag cgctaccaca
     1141 tcttcgagaa gttcatgacc gtgctggtcc tgatcaagtt cgtctccgtg gtgtcgatcg
     1201 ccttcctcgt ctcgcctgat ctcggtgccc tcgcgaacgg cctggtcccc cggctccccg
     1261 agggctcggt gatctacgtg ctcggtctca tcggcggcgt cggcgggacc atcaccatgg
     1321 cggcctatgg ctactggatg atcgcgaagg gctggaaggg cacgggctgg ctgtcggtga
     1381 tgcggctgga caacgccgtc ggctacatca tgaccgcgat cttcgtgatc gccatgctca
     1441 tcgtcggagc ggagatcctg ctcgggcagg acctcaccga gagcgacacg ggcctgctca
     1501 cccttggcac cgagctcggc gcgcgctacg gcgactgggc gcggatcctg ttcctcgtcg
     1561 gcttcctgtc ggtgaccacc acgtccctgc tgggtgtctg gaacggtgtg agcctgctgt
     1621 tcaccgactg gacgcggacg atccggctgc cgcacggcgc gaaggccgag ctcgacttcg
     1681 acaccgaggc gctccgaact ggcccggcca ccgccacggt gacgggttac gaggccaggg
     1741 tggccgagcg gagcctgccc ttccgcgcgt acctgctgtg gctcacgctg ccgccgatgg
     1801 cgctgctgtt cctcgaccgg ccgttcgccc tcacgctcgt ctacggagtg ctcggtgccg
     1861 ccttcatgcc gttcctcggg gtcacattga tcatgctcct caactccagg cggatcgctc
     1921 gggagggccg ctccgggtgg ctgtccaacg ctctgctcac cgcggcgtcg gcgctgttct
     1981 tcgtgctcct ggtgacggac ctgatcaacc ggttcgggtg acagtgcgac aaacatgaca
     2041 attaggaaca tctgagcatc aaccgggaga acagctcgtc ccgtcatatt acaggcgatc
     2101 acacttcggg ttaccgatcc tttgaccacc tgaagcggaa gtattcgtga acttccgacg
     2161 aaagccgctc tcgtacactt tcctgtcgct ttcgcttccc atactgccct cgtattgacc
     2221 gttcgagcgg caggagagca agggtgcccg gcttcgcagg caaccgctgc atcgccggag
     2281 tgcccgccgt ggctgcgctc gcggtggccg cggcgatcgc gctgtggccc gcccccgctc
     2341 cagccactgc gcccgatctc gtcatctcgg tgtcgcggtc gtcgtgcggg caggactgga
     2401 cagacccgac gcccggtccg cagaccttcc aggtgcacaa caccggtgcg gtcacgacca
     2461 ctgtcgacct ggtcgatccc gcgacaggcg ggatcttcgg cgagatcgag ggcctgggct
     2521 ccggcaccac gcgctccatg ccggtcacgc tgggtgacgg cgagtacgcc ttccgctgtc
     2581 tgcccgacga cgccgacgcc gacgtgggac cgaccgtcct gttcagcggc agcggtgtgc
     2641 gatcgagtcc cgccgtggtc ccggttacgc gcaacgacct cctcggaccc gcgaaggact
     2701 atcgggaaaa ggtgatcacc gggctcgacg cgctcgtccg ggacaccggc gcactcgcca
     2761 cgacagtcca ccacggggac cggcaggcga gcgaggcggc atggctcacc gcgcacctgt
     2821 cctaccagca gctgggcgcg gcctacggcg ccttcggcga cgctgcgtcc gcgatcgacg
     2881 gcaccgccga cggcctcgcc ggcggcacct cggatcccgc cttcacgggc ttccatcgcc
     2941 tcgagttcgg actgtggcac cgcgcgccca tggctgcgct ggccgcggtg gccgaccggc
     3001 tcgacgccga cgcgcgcgct ctgcggcagt ccttccctgc cgcgcagatc gatccgaacg
     3061 acctggcgct gcgcgcccac gagatcatgg aggacgccct gcagttcgag gtcaacggcc
     3121 gtgccgacta cggcagcgga accaccctcg ccaccaccct cgcgaacatc gacggcaccc
     3181 ggaccgcgct gaacgtgctg cgccctctgc tccagcagcg gttgcccacg ctccccacgg
     3241 tggacacctg gctggaccgc gccacctcgg ccgtgcaagc cgcatggcgc cccgacggga
     3301 cctggacgcc ggtcggcgcg ctcgaccaga gacaccgcga ggagatcgac ggcgcggtgg
     3361 cggagctgac cgagcagctc gcgccggtgg ccacgatcgt cgcgccgcgg cgggtctcgt
     3421 gaccgcgagg ctggggcgcc gctcgttcct gcgccgggcg ctggtcgggg ccggcgtgac
     3481 ggccgcggcc gtggccggcg gcggagtggc cacagccgcc gacggagaca gcgacgctcc
     3541 ggcgatcccg ttccacggcc cgcaccaggc cgcgatcctc cggccgccgg ccgcccggag
     3601 catcgtcgcg gcgttcgaca tcatcgctgc cgaccgggcc gacctgaccg acctgctgcg
     3661 caccgtcacc gaccgggccc ggttcctcgc cgcaggcggc gagccgccgg cactggggat
     3721 caccgcgccg ccccgggact ccggcgtgct cggcccggtt gtgccgtccg gggacctggc
     3781 cgtgatcgtc ggcgtcgggg tgtcgctctt cgacgatcgc ttcgggctcg ccggcagcag
     3841 gccggcgcgg ctgacgacga tgccgacgtt cccgaacgac gcgctcgacc cagcccagtg
     3901 tcacggcgac ctgagcctga cgctctcggc gtcctcgccc gacactgtgg tgcacgccct
     3961 gcgcgacatc gcccgcgcca cccgcggggg catgcagctg cgctggcgcg tcaacgggtt
     4021 cggctcacca ccccgcccgg ccggcacccc ccgcaacctg atgggcttca aggacggcat
     4081 cgcgaacccc gacgtggacg acgcgcccga gatggaccgc ctggtgtggg tgggcagcgg
     4141 gcagtcggac gaaccggcgt ggaccggcgg cggaagctac caggtgatcc gcgtgatccg
     4201 catgttcgtg gagttctggg accgcgtgtc gctcagcgag caggagaacg tgatcgggcg
     4261 ccgccgggac accggtgccc cgctcgacgg cacggcggaa ggcgacatcc cccagttcgc
     4321 ggacgatccg gtgggcacgg tgatcccgtt gacgagccac atccgccggg cgaacccgcg
     4381 cacgcagcag acggatgcga gccgaatcct gcgccgcgcg acgaactacg accgtggaat
     4441 cgacagcaac ggcaacctcg acatgggcct ggtcttcatg tgttaccagc aggatctccg
     4501 gcgccagttc gaggcggttc aggaacggct gaccgatgag cccctcgtcg actacatctc
     4561 accgtttggc ggtggctatt tcttcgcatt gcccggcgtc accggccccg gcgaccacta
     4621 cggccgagcg ttgctctcct gattcacccg cccccgatcc caccagaaga atttccccga
     4681 aacaggagga caagtgggaa ccaaatccag caggtggcgc cgagggcgtt cgctgctcgg
     4741 tgcctgcgcg ctggcctcgg ctgccgtgct cgctgcggtc accgtctcca cggcgaacgc
     4801 cgcagacacc ccgcctacgg ccggcaagca cgccgaccag ttcaacacgg cgacatcgat
     4861 caagcacgtc gtggtgatct tcggtgagaa tgtctcgttc gaccattact tcggcaccta
     4921 cccgaacgcc gccaacaccg acggcacccc gttcaccgcc gccaagcaga cccccaagat
     4981 cgacgggctc acacccgatc tgctgcagcg caaccccaac gcctacaacc ctcagcgcct
     5041 gtccccggac caggcgctga cctgcagcca gaaccacggg tacggcgccg agcaggccgc
     5101 gttcgacggc ggcaagatgg atcagttcgt cgagaagacc gaaaccgaca catgcagcgg
     5161 acagccgatc gtgttcggtc agccgggcct ggtcatggac tactacgacg gcaacaccgt
     5221 caccgcgatg tggaactacg cgcagaacta cgcgatgagc gacaactcct acaacacgac
     5281 gttcggcccg tccacccccg gtgcgatcaa cctcatctcc ggccagacgc acgggacgca
     5341 ggccgtcacc tcggtgacgc acgacccgac gtcggacccc ttcgtcacgt ccccggacgc
     5401 caacggggtc ggcacggtga tcggcgaccc cgacccggcg tacgacgact gctcggacaa
     5461 gaaccacacg gccaccgaca acctcgccag ccagagcggc aagaacatcg gtgacctgct
     5521 gaacgagaag ggcgtcacct ggggctggtt ccagggcggg ttccgcccga cgggcaatgc
     5581 caacgggttc gcagtgtgcg ggcagtcgca ccagaacatc gggggcatca gcgtcaccga
     5641 ctacagcccg caccacgagc cgttccagta ctacgagtcc acggccaact cgaagcacct
     5701 cccgccgacc tcggtggcgg cgatcgggca gaccgaccag gccaaccacc agtacgacac
     5761 ggccgacttc gacgccgccc tcgcggcggg gaacacgcct gcggtcagct tcctgaaggc
     5821 cccggcctac gaggacggcc acgccggcaa ctccagcccg atcgacgagc agcggttcgt
     5881 cgtcgacgag atcaacaaga tccagcagtc gaaggactgg aagtcgacgg cgatcgtgct
     5941 ggcctacgac gactccgacg gctggtacga ccacgcgaac gcgatcgtca acggctcgca
     6001 ggacacgacg caggaccaga gcatctgcag cggcacgccg gtgcggctgg gcggctacgc
     6061 ggaccgctgc ggttacggtc cacggctgcc gctcgaggtg atctcgccgt tcagcaaggt
     6121 gaacttcgtc gaccacaccc gcaccgacca gacctcggtg ctgaagttca tcgaggacaa
     6181 ctggtccacc ggccggatcg gcgactcgtc ctacgacgcc tccgcgggag atctgacgag
     6241 catgctcgac ttcaagggcg ccaaggcccg tgcggtgacc ctggaccccg gcaccggcgc
     6301 cgtggtggac gggaagccgg gcaagggcaa cggctgacgg tccgcgcggc cactgctcac
     6361 ccggagacca ccctggtcgc gttcgccgcg accagggtgg tgcggcgcag cacgaccagg
     6421 ttgacgacct ccgggcccgg tgttacgaat gttgaacacg caattaagcg cggagggccg
     6481 ctccgtgcgc ccacccgggg agaacgtcgt gaccatggcg aacaccgccc gcaccgtcga
     6541 ccgggtcgag gacggcctgc acgccgggcc cgacgcgcag gtcgacgaca agcgtctgct
     6601 gatcctgccg accgatccga ggcgcaccga ccccttcctg gtgctgtccg aggactggtt
     6661 ctcgacaccc ggcttcgagt ggcacccgca tcgcgggctg gagaccgtca cgttcgtcct
     6721 cgacggggtg ctcgagcacg gtgacaacat cgggaacgcg ggtgcgctgg agtccggtga
     6781 cgtgcagtgg atgaccgcgg gccgcgggat catccaccgc gagctcgcct accgcaacga
     6841 gcgcgcgcac accctgcagc tgtggctgaa cctcccagca ggcgcgcgca tggtcgacac
     6901 ccgttaccag gacgtcctcg ccgcaggccg gccgcggatc acccgaccgg gagccgtcat
     6961 cgacgtcgtg tccggcaccg tcgacgaggt caccgggcct gcgctgaacc attggccggt
     7021 acagggcgtg atcgtcacta tcgaacccgg cgtcgtggtc gacctgccgg tgcccgcacc
     7081 cgatcgggcg ttcgccctgg tccttcacgg cagcaccgcc gtggccgggc accaggtgaa
     7141 cgccgggcag gtggcgtggt cggaaccggt caccgggccg ggcagcgtgc tcaagctgcg
     7201 cgcggccccc gacaccgacg acgtggcgcg actcatggtc tacagcgggc cgccggtcgc
     7261 agagccgatc gtcatgggcg gctcgttcgt gatgaaccac gcacacgaga tcgagcaggc
     7321 ccgccgcgac ttccggtccg gcgggttcgg gccggtgccg aggctcgcgc gactctagcg
     7381 tcgcccgtca gggcgtgcgc agcagcatgg ccgtcatcgc cgcagccagc accacatgcg
     7441 acacggcggc ggtctgcggt gcgcacgccg agaacgcggg cgcccgcagc gccacggctg
     7501 cgttcggccc gtccctcccg tcctggacgg acacggccgg gtgcgcgtcg gggcatgtgg
     7561 tgcgacagcg gtcggcccag cggagggcat gccaggcgac gtggccggcg agcagcatcg
     7621 ccggcgccgc gacgagcccg accgcggcga ggacgaacac cgcaggccac accgctgtcg
     7681 gcacaggatc gccgagcggc gagaacatgg ccgccatacc gagagcacgg ccgtgtacga
     7741 gacctcgccg aacgacgtcc cggcgcagcc gcgccagccg gacgaggtgc agcaggccgg
     7801 cagcgaggca gaccacagcc agcgcggcgg acgtcgacgc catggttgtc ctcccgaccc
     7861 cgttcggagg gcagcccgat ccaccagaac gacaggtcag gaattaggcg cgattccata
     7921 ccgtgctggg gtaggggctg gtccgtcggg cgtgtgacga gcaggggtcc atgccggaat
     7981 gggtcttgca gagtggccca cccgcgttcg aaggtgttgc cttccccttc tgggcgagta
     8041 cggcggaggt ctgaagatgg cgggagagtc ccgggtggtc cgcgcagcgc tggtgcaggc
     8101 gaagtggacc ggcgacaccg catccatggt ggaggtgcac gagtcctatg cgcgcgccgc
     8161 ggcggaacag ggcgcgcagg tgatgggctt ccaggaggtc ttcaacgcgc cgtacttctg
     8221 ccaggtgcag gaggcggagc actaccgctg ggccgaaccg gtgcccgacg gacccactgt
     8281 gcaacggatg caggcgctgg cccgcgagac cggcatggtg ctcgtggttc cggttttcga
     8341 gttggaagcc tctggcttct actacaacac cgcggcggtg atcgacgcgg acggtacgtt
     8401 tctcggtaag taccgcaagc accacattcc acaggtcacg ggattctggg agaagttcta
     8461 cttccggccg ggaaacctgg gatggccggt gttcgacacg gcggtgggca aggtcggggt
     8521 ctacatctgc tacgaccggc acttccccga gggctggcgc gcgctgggcc tggcgggcgc
     8581 gcagatcgtc tacaacccca gcgcgaccag ccgcgggctc tcgtcgtacc tctggaagct
     8641 ggagcagccc gccgcggccg tcgccaacga gtacttcatc gccgccatca accgggtcgg
     8701 ggtggaggag tacggcgaca acgacttcta cggcaccagc tacttcgtcg accctcgcgg
     8761 ccagttcgtc ggcgagcctg cgtcgggcag cgacgaggag cttgtggtgc gcgacctcga
     8821 cctcgggatg atcgacgagg tgcgccagca gtgggcgttc taccgcgacc gccggcccga
     8881 tgcgtatgga ccgctggtgg agcaatgagc gagcttgcgc gcgaatcagt gtcactgcgc
     8941 ccgcgcgctg atacgtctca cgatgggccg accgagcaca gcgagggagt gcgatgagtg
     9001 ctcaggagct gttcgcccgt acccgcgcag tcctgccctc ctggctggcg gtctattacg
     9061 aggagccgat cgagatcgag cgcggcgagg gccgctacgt ctgggacgcc gacggcaagc
     9121 gctacctcga cttcttcggc ggcatcctca ccacgatgac ggcacacgcg ctgcctgagg
     9181 tgaccaaggc cgtcagcgag caggccgggc ggatcatcca cagctccacg ctgtacctca
     9241 accggccgat ggtggagctg gccgagcaga tcgcggcggt ctcggccatc cccgacccca
     9301 aggtcttcct gaccaccagc ggcaccgagg ccaacgacgc ggcgctgctg ctcgccagca
     9361 cgctgcgccg gtcgaaccag atcctggcgc tgcgcaacag ctaccacggc cggtcgttct
     9421 ccacgatcgc gatcaccggc aaccgctcgt ggtcgcccac ctcgctgtcg ccgttccaga
     9481 cctactacgt gcacggcggc cagcagtacc gctcgccgta cgcggggttg tccgacggtg
     9541 agtacatcgc cgcgtgcgtg gccgacctgc aggacgtgct cgaccagtcg ggcggtgaca
     9601 tcgcggcgtt catcgccgag ccgatccagg gcgtcggcgg gttcacgtcg gggccggacg
     9661 ggctgctggg cgcgttcgcc gcggtgctgc gcgaccgcgg gatcctgtgg atcagtgacg
     9721 aggtgcagac cgggtggggc cgaaccggcg accacttctg ggggtggcag gcccacaccg
     9781 ccgaccacgg cgacgccact cccgacatcg tcaccttcgc gaaaggcgtg ggaaacggat
     9841 tgtcgatggg cggggtgatc gcccgcgccg agatcatgga ctgcctcggc gccaactcga
     9901 tctcgacgtt cggcggctcg ccgctcaccg ccgcgggggc cctggccaac ctgcgccacc
     9961 tgctcgacca cgacctgcag accaatgccg cccggcacgg tccggcgctg ctcgacgggc
    10021 tccgcgcact ggagagcccg atcgtcggcg acgtccgtgg caaggggctg atgatcggca
    10081 tcgaactggt gcgcccggac acgggcagcc ccggcgagcc cgcacccgag ctcgcggcgg
    10141 ccgtgctgga ggccaccaag cggcgcggcc tgctcgtcgg taagggcggt ctgcacggca
    10201 acgtgctgcg catcgccccg ccactcagtc tcaccgctga cgaggccgcc gagggcctcg
    10261 cggcgctcgt cgcctcgatt caggaggtca cgccatgacc cgcacgctga tccgcaacgg
    10321 cctcgtgatc accgcgtccg acgagctggc gaccgacgtc ctgatcgagg acgagacggt
    10381 ggtcgccctg gcggacccgt cgatctccga agggtggacc gccgacaccg tcatcgacgc
    10441 caccggcaag tacgtcatcc cgggcggcgt cgacgcgcac acgcacatgg agctgccgtt
    10501 cggcggcacg ttcgcctccg acaccttcga gaccggcacc cgcgcggcgg cgtggggcgg
    10561 caccacgacg atcgtcgact tcgccgtgca gtccatgggt gggtcgctgc gcgagggcct
    10621 cgatgcctgg cacgccaagg ccgacgggca gtgcgccgtc gactacgcgt tccacatgat
    10681 catggccgac gtgaacgagg gctcgctgaa ggagatggag accctcgtcg gcgaggggat
    10741 caccagtttc aagctcttca tggcctatcc cggcgtcttc tacagcgacg acggcaagat
    10801 cctgcgcgcg ctgcagaagg gggccgagac cggcgggctg atcatgatgc acgccgagaa
    10861 cggcatcgcg atcgacgtgc tcgtggagca ggcgctggcc cgcggcaaca ccgacccgcg
    10921 ctaccacggg gtcgtccggc acgccctgct ggaggccgag gcgaccagcc gggcgatcaa
    10981 gctcgctcag gtcgcgggcg cgccgatcta catcgtccac ctgtccgcca tcgaggcgct
    11041 cgccgagctg acgcgggcgc gcgacgaggg cgtcaacgcg ttcgcggaga cgtgtccgca
    11101 gtacctgtac ctctcgaccg acgatctggc gcggccgggc ttcgagggcg cgaagtacgt
    11161 ctgctcgacg ccgttgcggc ccaccgagca ccaggcggcg ctgtggcggg gcctgcgcac
    11221 cgacgacctg tcggtggtgt ccaccgacca ctgcccgttc tgcttcaagg gccagaagga
    11281 gctgggcctc ggcgacttct ccaagatccc caacgggctg cccggcgtcg agaaccggat
    11341 ggacctgctg caccaggccg tgctcgacgg acacatcgga cggcggcgct ggatcgagat
    11401 cgcctgcgcc acgccggcca agatgttcgg gctctacccg cgcaagggca ccatcgcgcc
    11461 gggcgccgac gccgacgtgg tgatctacga ccccgccgcg cagcaggtga tcagcgtcga
    11521 gacgcaccac atgaacgtcg actactcctg ctacgagggt cgcaccgtca ccggcagggc
    11581 ggagaccgtc ctgtcccgtg gtcgggtggt ggtcgacaag ggcgagtacc tcggcgcgcc
    11641 gggccacggg cggttcctgc gccgcgacac ctgccagtac ctgcgctagg ggacgcgcga
    11701 tggacatcgg actggtcctg cagaccgacc cgcctgcgtc gaccgtcgtg gacctgatgg
    11761 tgcgggcgga ggagctcggg ttcagccacg gctggacgtt cgactcccac gtgctctggc
    11821 aggagcccta cgtcatctac tcccggatcc tggagcggac gtcacggctg ctcgtcgggc
    11881 cgatggtgac caaccccggc acccgcgact ggacggtcat cgcgtcgctg cacgccacgt
    11941 tgaacgacat gttcggcaac cggacgatct gcggcatcgg gcgcggcgac tccgcgatgc
    12001 gcgtgcaagg gcggccaccg accacgctgg cccggctcga ggcgtcgatg cgcgtcatcc
    12061 gggagctggc cgagggccgc gcggtcgacc tcgacggcac cacggtgcag atcccctggg
    12121 tgcgcgcggg cgcctcgctg ccggtctgga tggctggcta cggccccaag gcgctcgacc
    12181 tcgtcggccg ggccgccgac gggttcatcc tgcagctcgc cgacccttac ctcgtggaat
    12241 ggaccgtgaa gacggtgcgc gccgctgccg aggcggcggg ccgcgacccg gcggcgatca
    12301 cgatctgcgt cgccgctccc gcgtacgtgg gggacgacct cgcccacgcc cgtgaccagt
    12361 gccgctggtt cggcgggatg gtcggcaacc atgtcgccga cctggtgtcg cggtacggcg
    12421 agagctcgga tgcggtcccg gacgtgctca cgggctacat cgcgggccgc cacggctacg
    12481 actactccca ccacggccgc gccgacaacc gctccaccga cttcgtaccc gacgaggtcg
    12541 tcgagcggtt ctgcctgctc ggcccgccgg ccgcacagct ggacaagctc gcccagctgc
    12601 gggagctcgg ggtggaccag ttcgcgatct acgccatgca cgacgcccgg gaggagacga
    12661 tcgaggcata cgggaggtcg gtgatccccg cgctgggctg agggcgccgc atgtgcgacg
    12721 cgccgcagtg ggcgagctgc gcacgaggtg gagctcgccc accgtcaatg gtcagcgcgc
    12781 aagctcgctc accgaatgcc gaccacgtcc aggggggcgg cctcgggcgc cgtcgttcgc
    12841 gggctggagg atgccgggcc cgtcgtgggg gcgcccagcg ccaccgtgac ggcggcccgc
    12901 gcaccggccg tgacgtgcac gacccgcgtc gccggagcgt atccggtcgt ggtgagggtg
    12961 taggtgccct cggtgaggtc gtctaaggcg aacgtcccat ccgggccggt gaccgtcgag
    13021 gccacgaccg tgccggtgct gtccaccagt gtggccatcg cctccgggac gccatgtccg
    13081 gtgctcgccg tggtgactgt gccgagcagc cgcgagcgct gcggcagctc gatgtcgcgc
    13141 ttgatcgcgg ccccgatgtc cagccgcacg ctcaccgcga ccggctgccg gccggggctc
    13201 gcggcggtca gcgtgtagat gccgtcgggc accccggcca gtcggtagcg gccgtcgggc
    13261 tccgccatag cggcggcggt gacgtcgccc tgtgcgtcga tcagtgtgac gttcgcgccg
    13321 cccactgagc ggagtccgcc ggcggggtcc ggcgtctgca cgactccgaa cacggagctg
    13381 gtgccggtga gcgagacgtc gtgccgggac ggccggtccg ccacggcgac cagtccggca
    13441 tggggctggt aggtgccggt ggccacgacg acgaggtaca tgcccccgct aggcagggcg
    13501 acgcggtagc tgccgtcggc ggcggtgctg gtgcgggcct cctgccggcc ggccgggtcg
    13561 gtgacggtca cgacgacctg cgcgagccgg ccgccgtcac cgcggctgac gatgccgaac
    13621 acgccgggac cgtcgcggta cgaagatgcg tctacgcgcc cggacatcgg tgctgtgaca
    13681 atcgaaccga tggcgcccgc gaccggggtg ccattcccgt tgcttcggcc gttcccgttg
    13741 cttcggccgt tcccgttgct ccggccgttg ccgttgcttc cggagagttg cactccggac
    13801 tcctgggttg acagcgtgtg gcgccctgcg tctttaccgg caccggcgcc ggcaagcaca
    13861 aggaccgggg ctggaccggt gggctggccg gggtgagcgg gcacggcgcc ccccatcgtc
    13921 ccggccgcct cgggagcgct gccgcgcagc ggcacctcgc ggatgatcag cgccgcgagc
    13981 acagcgacga gtcccacgat tccggcccac atgaacgcgg tggtggtgcc gctggcgact
    14041 gccgcctggt aggcgtcgcg gactgcggcg ggcaggccgc cgagcatggc cggggtgatc
    14101 tgcccgcccg tgacctgctc gccgcccgct gtgccgagcc ggtcggacaa cgtggcggtg
    14161 agcctggtgg tgtagaagac gcccagcagc gacacgccga gcgagccgcc gaccgtgcgg
    14221 aagagggtcg atgcgccgct ggatgcgccg atgtcgcgct gcgccacgct gttctgcatc
    14281 accagcatcg tggtctgcat cagcaacccc atgccgacac cgagcaccac catgaacagc
    14341 gacgtcgtca gctgcgtggt ggccaggccc atcagggaca gcagcccgag gcccgcagcc
    14401 atcaacagcg agcccgtgat gatcacgacc ttgtagcggc cggtccggga gacgagctgc
    14461 ccgctcagca gcgaggtcac cagcatgccg cccatcagcg ggagcagcag cagcccactg
    14521 ctggtcgccg acgcgccctg gacgatctgc tggtactgcg gcaggtacgt caccgcgccg
    14581 aacattgcga aaccggtgag gaacgtgacg accgtgacgg cggagaagtt gcggttgcgg
    14641 aacagctcca gcgacaggat cggttcgctg acccgccgct cgatgcgcac gaacagcgcc
    14701 gtcccgacga cggtgagcac gcccaggccg acgatctgcc acgaacccca cgggtactgg
    14761 ctaccgcccc agctggtgag cagtgtcaac gccgagatcg cgacggcgag cgtgcccgcg
    14821 cccagccagt cgatcttcgc ctcgcggcgg acccgcggca ggtgcatggt gaaccacacg
    14881 acgatcagtg caacgacgcc caggggcagg ttgatgtaga acgcccagcg ccagctgagg
    14941 ttgtcggtga ggaacccgcc gagcagcggg ccgccgatga acgccagcgg catcaccgcg
    15001 gccatcaggc cctggtagcg gccccgatcg cgcggcggca ccaggtcgcc gatgatcgcc
    15061 gtcgcgccga ccatgagacc gccggcgccg atgccctgga tggcccggaa cacgatgagc
    15121 tccatcatgt tctgggagag acccgagagc gccgagccga ccaggaagac cacgatcgag
    15181 gccatgaaga tgcccttgcg cccgtacagg tcgccgagct tggcccatac cggtgtcgag
    15241 accgcggtgg tcaacgcgta ggcggtcacg acccaggaca ggtgttccag ccctccaaga
    15301 tcaccgacga tcgtcggcat cgcggtgccg acgatcaggt tgtccagcat cgccagcagc
    15361 atcgcgatca tgagaccact catgaccgtg tatatctcgc gcttgctccg cacgggcggc
    15421 gcttcccgca tgtcaacgtc ggggacggtc tggcccatct cgctctccca ctccacggtc
    15481 tgccgcgtcg cacggcacca aactaactag tcgacaggct agcacccgaa actagcccgt
    15541 cgacaagtaa gatgtgtgac atgagcagca gcaccgatga gcggcggacc aacacccgcg
    15601 agcagatccg ggcagtcgcg ctggagttgt tctccgagcg cggctacgac aagacgtcgc
    15661 tgcgtgagat cgcggagcgg ctcggcgtca cgaaggcggc ggtttactac cactaccgca
    15721 caaaggagga gatcctctcc agcctgctcg acgagcatct ggcaggcatg gacgccatcg
    15781 tcgaatgggg acaggcccag ccgcccggtc ccgaccgtcg ccgggagatc ctgcagcgct
    15841 acagcgcgct gctggccggt ccgaccgacg aggcaccaca gctgacccgc ttcctgcagg
    15901 agagccagac ctcgatcaaa gagctcgccg cgggcgccgg tatgcgggaa cggttccagc
    15961 agctcagcgc gctcctcgtg gagccggacg ccgagctggt cgagcagctg cgtggccggc
    16021 tcgccctcgt cgccctgcac ttcggcgcgt tcgcgctcga cgagatccct ggcacccgcc
    16081 tggaacggcg cgacgcggcg ttgcaggtgg cactcgagct cggccttccc tgatgccgtt
    16141 ggcgtggcgg acaaacgcgt acgtgcaggc attcgctggt gcggaggtcg gcgggtacat
    16201 gatgcggggt gaccagcccg gtgcgtgagc ggccgcagcc gtgcggctcc tccgacagca
    16261 ggtactcaca ccgtgcggat cgtcgccatc atcgccatgt cctcgtgctc caggttgtgg
    16321 cagtggaaga cgaaccggcc ggcgtagtgc tcgaaccgca ccgcgacgtc gacgacctcc
    16381 gcgggccgga tgtccactgt gtccttccag ccgatgtcgt agggccccgg gccctcgctg
    16441 ccccgccgca acacctggaa cgcgtcgagg tgcacgtgca cgggatggtg gacgtcgctg
    16501 acgaaccgcc acacctcggt ctccccgagg gtgatgtcgg ccaatgagcg ggccgggtcg
    16561 aaggaccccc cgttgatcgt ccagccgcgg tggtcgccga ccgagccgcg ggtgaaccgg
    16621 aacgtccgct gcggcgcgcc cgcgggcggg acgagcggct ccacgtccgc gagccggtcg
    16681 ggaacccggc tgtcgtcggg agcggtccgg accacccgga accgcatcac gtcccgcgtc
    16741 cggcccgtgc ccagcgggtt gatcatcgtg acctcggagc cggccggcag tgccgtgaag
    16801 tcgacgacga cgtcgaaccg ttcacccgcg gcgatctcca gcgcctgatg cgtcaccgga
    16861 gcggcgagga gcccgttgtc ggagccgatc tgaacgaacg gcaggtccgg cacccccggc
    16921 acgcgcagca ccagccggta ccggcgggcg ttcgaggcgt tcagcagccg cagccggtag
    16981 cgcgacgcgt cgacctcgtg caccggccac ggtgccccgt tgaccagcac gacgtcgccg
    17041 agcacgccct ccatccagtc gtcctcaacg cccggcaacg agcgcatccc ctggtccagt
    17101 cccggatagg cgaaagaacc gtcggcggcg aacgcgcggt cgcagatcat cagcggcagc
    17161 tcgcgctcgc cgcggggcag gggcagcgcg tcctcgatgt cgtcgcgcac gaggtggaag
    17221 ccggcgagcc cgcggtagac ggacggaccg gtgaagtcca tgcggtggtc gtggtaccag
    17281 agcgttgccg cgcgctggtc cgtcgggtag cggtactcgc gctcgcccgc ggccacgtcg
    17341 ccgagcatcc cgtggtgcgg tgtccagccc gccgcgttcc cggcgggaag caccagatcc
    17401 agcggccaac cgtcgctctc ggccggagtg tgcccgccgt gcaggtgcac gaccgtgggc
    17461 accggcaact cgttgcggtg ctgcaccacg acgaggcgac ctcggcgggt ctcgaacgtc
    17521 ggtcccggga aaatcccgtc gtacccgagc acgggggtgc gcagtccggg aaggatctcc
    17581 acgttggcga cgcgctgcgt cacgcggtag aggtccgccc ccgtcggatc cgtacctacc
    17641 gggcgtgcca cgggagacac gggcagcgga acgcggaacg cgtcgggcag cggcacggca
    17701 ctcgtgagct gctcccccgt ctgaccgggc gccgaaccca gcccgcagcc ggccagcgcg
    17761 gccccggctc cagccgcccc gagcagcccg aggaagccgc ggcgcgagag ccgggtcatc
    17821 gccgggtccg cggcagtgcc gccgacgggg tccacaccca gatcgccagc agcaccgagg
    17881 tgacgacgat cgcgacgccg agcgggatgt gcagctccag ctgccgcgtg taccccgcag
    17941 cgatctggaa cccctcggcg aggaccagca gcaccgccac cggcagcacc cagagccgtc
    18001 cccggccgcc gagcacgtag gccagagccg caccgatgac gacgagcccg accagcgcca
    18061 ggagcgtccc gacgaggccg tgcacgccga tcgcgtcgac gtcgccggtg aggtacagcc
    18121 cggccagtac cggctgggcg aggacggcaa ggaggtgcac ggtgatcagc gatctgagca
    18181 accacaggga gatccggagc gcgcgagcga cgggcgccgt aggcgcgaga gtgtccacgc
    18241 cgaggatggt atgagtagac aatctacata tacatcaggg attcccctga accatgacct
    18301 gatgtgccgc cggaacgggt aggtagaatc cctactcgtg cgacctgacg ccgttcgcgg
    18361 tcatctcgac gggttgatcc tcgccgtact cgaggacggg ccccggcacg ggtacgcgat
    18421 catcgaggcg ctgcagacgc gcagcggcgg cgccctggaa ctgccgaccg gcacggtcta
    18481 tccggcgctt cgccgcctcg agcgcgcggc cctgctctcg ggcacgtgga gcaccgtcgg
    18541 cggccgcgag cggcgcacgt acacgctcac gcgcgccggg cggcacgcgc tggcgaacca
    18601 gcgggccgac tggagcgagt tcagcgcggt ggtggacgcg gtgctgcggc cggccgccgg
    18661 gcgtcctgcg gcgggcatgc cgtgaccgtc acggatccgg tcgaccggca cctcgccgag
    18721 ctcgagcgga ggctgagcgg accctcccgc acccggcgga gcatgctgag ggaggcgcgc
    18781 gacgggctgg tcgacgccgt cgccgcgcat cgcaggtgtg gcctcgacgc gcacgaggca
    18841 gcggcgaagg cggtgcgcga cttcggctcc gtccgcgaga tcgcaccgct gtaccaggac
    18901 gagctcgtgg ctcgtcaggg ccggtgggcc gcgctgctgc tccttgtcgt cttcccgggg
    18961 ttgactctcg gctggggcat gctgtggcgc ctcggcgtga ggtgggaatc ggagccggcc
    19021 tcggatctcg tcgtggcgct ggcccgcctg caggacacag cgagcgcggt ggtggcagtg
    19081 gtggccctcg tcctgctcgc ggtcacgttt cgccgggcgg cgcccgcacg gcaggtcgcc
    19141 gtgctggtga gtctcaccgg ggtggtcgga gctgtggtct gcggcggcac cggcctggtg
    19201 atgaacctgg ccaacggttc cgcggggcgc gagctggtcg aggcgaaccc gtacgccgtg
    19261 ccggcgttcg gctgcagcgc gctcttgtgt gcgttggtgc tccggtcggc ggtgcggacg
    19321 ctactggtgg ctggtagccc tgtaccgacg gcggccgagc gctgagatcc cgatccacct
    19381 cgctacccag acaggagatg gcaaggattc ctgtcaccgt tgggtcatga ccgcacagaa
    19441 caacccactc accgaccgcg tggcgctcgt cgcgggcgcg acacgagggg ccggccgggg
    19501 catcgcggtg gagctcggcg cggcgggggc aaccgtgtac gtgaccggcc ggtcg
//
LOCUS       DASRSC010000082        22824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5755501,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000082 DASRSC010000000
VERSION     DASRSC010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 22824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22824
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5755501"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1559
                     /locus_tag="VFE39_04085"
     CDS             198..1559
                     /locus_tag="VFE39_04085"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET6257811.1"
                     /translation="MMTRTTLRAALLMAVLCLLGSACSTPTPPPLARREVASDTVIDP
                     ARPCVREAPVPVPSPQAEPADQAGSGGLGATFDAGTNKIVLSAGQGITLPALSQAIDD
                     PRAVREVAPGEWLLGATIDVLPGASLRIRAPDVRWLMLSSSPAGFVSVKALGGGLDIT
                     GTCVTSWDPMQGRVDTEHLDGRSFLLARNGGQLTIDHAEVRYLGYGEVEAYGMSWRVQ
                     GTSGHITNSIVSHLFYGLYTFEISGLVVQDNEFHDNVLYGIDPHTNSHNLLIERNIVH
                     DNGKHGIILAEDCTDSVIRDNIVYRNEHHGIVLYLNSDRNLVEGNKSFANAATGININ
                     QSAGNTVRGNHTYDNTESGIGVSQTAQDNIVENNQIRGNQQDGVRLVSEATHTTVRNN
                     IIGENARYGVYIDSTGPYALTGNTIFNNRTGIMFKNRTTTPPTDNQVFANDKADIANT
                     ESR"
     gene            complement(1628..3073)
                     /locus_tag="VFE39_04090"
     CDS             complement(1628..3073)
                     /locus_tag="VFE39_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HET6257812.1"
                     /translation="MADAARTEKTFFGQPRVLANLFGVEMWERFSFYGMQGILLIYLY
                     YSTAQGGLGIDQTAATSIVGAYGGLVYLSTILGAWLADRMLGAERTLFYSAVLVMIGH
                     LALSVLPGLLGVGVGLVLIALGSGGVKATATSLVGTLYAPGDVRRDAGFSLYYLGINL
                     GALVGPILTGLLQSGLGFHYGFGLAAVGMAIGLAQYSIGRKHLTGKAREVPSPLPASR
                     WPLVAGTAVAAVALIAVLAFTGVLTPDRLANVVVALSVIATIVYFVIILRSRRITVLE
                     RRRVLAFIPMFIASAAFWSLYQQQFTVVTIYSDEQLNRDIFGWEMPVSFVQSINPVFV
                     ILLSGAFAALWTKLGSRQPSSPIKFALGTAMMGVAFLLFLPMAGDDPGSAPLLGLIGI
                     LFVFTIAELLLSPVGLSLSTKLAPEAFRTQMVALFFLSIALGTAMSGVLAQYYSPTAE
                     AAYFGVLGAVAIALGLVLALLAPFLRRLMAG"
     gene            3310..5439
                     /locus_tag="VFE39_04095"
     CDS             3310..5439
                     /locus_tag="VFE39_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET6257813.1"
                     /translation="MTGRHDSSIPAPDVAALVASWAGYLLRPGYLPMSREELHQFLTG
                     AALRLAHAAAAEPVDLAAARRVGASIVHGGLHSADVLPATVAVLARHLVPVAQSAGAD
                     RPQETAPAIQAAVTDGYLAQLRARIRDEQEMTRRAEVTARRQIEGALRASEARFHAIF
                     ANAGIGIGIADMEGRIVEVNTAFAGMLGYTVDEFRRLTVGDLVYPDDASGRWDLYRDI
                     VEGKRHSARVEKRYRHRDGHAVWTDLTASLIRDAQGTPLYTVAMAEDATTRRDLQERL
                     QRQALHDPLTLLPNRALFQERLGAAFSRPGSHVGICYLDLDRFKAVNDRLGHDIGDAL
                     LIAVARLLDKYVSTRGHLVARMGGDEFVILVDHPPNGELAILADTILAVLTDPIDVDE
                     HRIVVSASIGVVECGVDETTPAEILKAADVTLYWAKSEGRNRWARFDPERNARDMTRY
                     TLSATLLPGLERDEFRVEYQPIVELVDGRVRGVEALVRWVHPTLGRLSPDQFIDLAEE
                     TGSIVPLGRRVLTEACERGAEWNAAHPEAELFVSVNLAVRQAHDPDLVDDVARALART
                     GLPPHLLQLELTESALLGPAGRPVEAITALAAMGVRIAVDDFGTGYSNLGYLPRLPLH
                     TLKLAGMFIEGLRNPATGADPIVGSLIALAHALGLVVTAEGVETQAQADRLRAGACDT
                     AQGWLYSRPASWDDLVHLLDRHLPPGS"
     gene            5664..6296
                     /locus_tag="VFE39_04100"
     CDS             5664..6296
                     /locus_tag="VFE39_04100"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6257814.1"
                     /translation="MSHGSGRIVVGVDGSSGGRAALAFAMQDAARRDAAVEEVVAFAT
                     AETLSALCDPPYRSFTTSAAEVRAAVEKKAAGIVEDVSGELAGELESLPPVAVTAVGG
                     GAVVALLRAAHDADLLVVGSRGRGGFASMVLGSVSMQCVLHASCPVTVVHPVAALPPV
                     ARRGLQLERARRGGGQAPATLTTDPETRSRRWIRKNVHIRVAARGTAWSL"
     gene            complement(6393..8117)
                     /locus_tag="VFE39_04105"
     CDS             complement(6393..8117)
                     /locus_tag="VFE39_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6257815.1"
                     /translation="MAQEQPETPRDVAERAASVLGPEGDLFEDMDAAGFGDALTQAVR
                     ASLASPGAPALAAINLATDLARIPFVATTRWLGGDAEPPLPVDPKDRRFADPAWDTNP
                     MFYSVRQAYLAACRFARDVVGSAELEADEARKAAMAMNLMLDALAPTNFLATNPAALK
                     RAFDTAGGSLLKGTRNFLDDLVNNEGRPRQVDTSGFEVGRNLARTPAKVVYRNDLMEL
                     LQYEPRTEHVHATPLLCSPPWINKYYVMDLAEDRSFIQWAVDHGRTVFAISYKNPSKS
                     MSDTTMDDYLVHGPQTALDVIEEITGAKTIDIVGLCLGGALTAITAAYLTQAGDHRIG
                     SLTLLNTMLDYAEPGVLGTFTDMRTVEKLEKKMRREGMLEGKSMAGTFDALRANDLIF
                     NYVVSNWLMGQDPPAFDILAWNTDSTRMPAAMHAFYLRNFYVENKLAAGELEIAGKTI
                     DLSAIKAPTYVVSAINDHIVPWKSAYKNVGLISGPVRFVLSSGGHIAGIVSPPGPKVW
                     HMVAETETTPATGDGWREVAERRSGSWWEDWSRWSDANSGPLQEPPRMGSAKYPVLGD
                     GPGTYVLT"
     gene            complement(8211..8699)
                     /locus_tag="VFE39_04110"
     CDS             complement(8211..8699)
                     /locus_tag="VFE39_04110"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6257816.1"
                     /translation="MPSGPILIGFDGTSAAERAVWEAGRLFAPRPAVVLVVLVPGEAF
                     AAVVGPLAGAGIPLTEVEIQAALEYEEKAMTTARQLAEKGAAMAREAGLEAEGRVVAD
                     DAGVPRTLLRVADERDASAIVLGSRRHRTLSEFLLGSTTRDVLKRARRPVVAVPDVSD
                     DR"
     gene            complement(9084..9356)
                     /locus_tag="VFE39_04115"
     CDS             complement(9084..9356)
                     /locus_tag="VFE39_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257817.1"
                     /translation="MSTPDPTRDLEAATQRVRELSEQVVEQAKKNGLAWMEGYERMLK
                     NLLDLEEQAAKGTGAEWASTLATAHANFVKDTSEVLFSAMRQQLKT"
     gene            complement(9567..10163)
                     /locus_tag="VFE39_04120"
     CDS             complement(9567..10163)
                     /locus_tag="VFE39_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257818.1"
                     /translation="MIDAHDERNPMPHFLVSGIAELVAQVATVLRENGSTVVEVDDIG
                     DVPRACSDAGDRAFDGYIQLPATFMVQGDTAVERVHHFFADGVLARFPAVSAALPALT
                     PEARITFVMGVLPPEVSTDDDVSARAALVRVLGHAARADGPAGLRVSVVGSGSTPKEI
                     ALTGMGRNPEWESLAAEASGGSYADWRVELLGLMSAET"
     gene            complement(10363..11208)
                     /locus_tag="VFE39_04125"
     CDS             complement(10363..11208)
                     /locus_tag="VFE39_04125"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HET6257819.1"
                     /translation="MAGGSKDPGRSVISKISAILLAVSEGSAPTLTEIAARSDLPLST
                     VHRLVTELAAWRVLERDGEGRYRAGPPLRTIGAGSACSGHEPDDGAATPAARIRDRAV
                     PVIEDLFRVTGAPVRVGFLDGAAVAYVEKAAGHLPVSLPSPAARLPAHATALGKVLLA
                     FSSPRTVDTLLSRELVRYTTSTVTRAERLRWSFRTIRAARIAVCDRELDQDWCGVAAP
                     AFGAGGDVVVAVELRVRDLARDVPALRAPLVVAAGWLSRELAPLVRPYAGVGPVPAPV
                     QRGSA"
     gene            complement(11659..12375)
                     /locus_tag="VFE39_04130"
     CDS             complement(11659..12375)
                     /locus_tag="VFE39_04130"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6257820.1"
                     /translation="MARPTALPSAPGPAQRATQPTLPAYSRDAATYDARTGPFDRYRR
                     QIVDLLPLERNDVVLDIGCGTGLCFAQVQEHIGPGGTIVGIDTSADMLTIAAERVTSQ
                     RWANVVLVEAAVEHADLPDGADHALFCAVHDVLQSGPALDHVLAHVREHGGVAAGGGK
                     WAPAWAMAVNAAVLALHTPYVRDFTGFDRPWTGLAERIGGMQVQEVGMGGGYMAYGEV
                     PRDAGHAVAVHSRCAWCGPP"
     gene            12594..12989
                     /locus_tag="VFE39_04135"
     CDS             12594..12989
                     /locus_tag="VFE39_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET6257821.1"
                     /translation="MAEVDKSEKITINVGLVDLGQIDLLVDEGFFANRTDFIRTAIRR
                     QLETRSDAMTDTIGRRALTLGTRHLARHELEQLRDAGRTTELRVLGLATIADDVDVEL
                     ALATIAAVEVLGAFRAPRDIKAALAPRTV"
     gene            13051..14046
                     /locus_tag="VFE39_04140"
     CDS             13051..14046
                     /locus_tag="VFE39_04140"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HET6257822.1"
                     /translation="MDHDPNIGMASALQLTWAGRLTEAVAVIQRTLGADGTRVVRGYG
                     CSSTRIHAQGGGQPVHLTHTEAAGTRRFDLYVPPGRTGERLPLVVMLHGGSQNAADFA
                     AGTRMTELAQQHGLLVAYPEQSRAVNRGGYWNWFSPAHQRAGAGEPSILAGISRRIVA
                     EYGADPSRVYVAGLSAGGAMAAVVAATHPELYAAVGVHSGIAAGAADDVGSAFAAMRT
                     GGTPGAGADLPLIVFHGDADTTVAPVNAERLIDARLSAGSRRTTAAAVRLAEPGRRPY
                     TRTVIRDQDDSVVAESWLVHGMGHAWSGGDPAGSYADPKGPDASAEMLRFFLEYA"
     gene            complement(14063..14902)
                     /locus_tag="VFE39_04145"
     CDS             complement(14063..14902)
                     /locus_tag="VFE39_04145"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET6257823.1"
                     /translation="MSESERQLQDSVREAPAVPPDIDITRPSVARGYDFALGGKNNFE
                     IDRLAVDALTNAFAGALPLARDNRGFLRRGVRYLVGEAGIRQLIDVGSGLPSAGNVHE
                     VAHELDPAVHVVYVDVDPIVLAHARALLADNNTTTVIQANAADPVSILEAPETQEFID
                     FDKPFAVLLSGILHHLNDDQDPFGVTRTFRDRMPSGSYLLASNFLDDDDPRAKAAERA
                     FIDGGFGTGRFRTWDEHRRYFDGLEMVEPGLTYANDWRPDGHTPADSPWHTFYAGGVG
                     RKP"
     gene            complement(15025..15927)
                     /locus_tag="VFE39_04150"
     CDS             complement(15025..15927)
                     /locus_tag="VFE39_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6257824.1"
                     /translation="MGQDQDQFTQQDPGEQHAAPGSGSETVAYPGRTGELDVRPDHGE
                     ETYRGTGRLEGKKAVITGGDSGIGRAVALAFAREGADVLISYLESEEKDAQETVRLVE
                     EAGRTAVTVPGDLREEAHCQTVIDRAVQEFGRIDVLVSNAAYQMSQDAGIEAITTEQF
                     DRVMRTNVYATFWVCKAAVPHMPEGSSIITTSSVQAFNPSPHLLDYATSKAAIVNFTK
                     GLGNNLTEKGIRANSVAPGPVWTPLIPATMSEEKVKSFGKQSPLGRAAQPAELAPIYV
                     FLASQESSYITSEVIGVTGGSPIT"
     gene            complement(16042..16758)
                     /locus_tag="VFE39_04155"
     CDS             complement(16042..16758)
                     /locus_tag="VFE39_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6257825.1"
                     /translation="MTEQDKCMRTELGAYVLGSLEPPERDAVDHHLADCAECRSELAM
                     LAPVAGLLGCLEPAEARNGMLTPSPALLPQALGAVRHHQQQDRRRLRRWKLVATAAAV
                     VAGMSGVLPQVLPTGASGPPPATVTASGTPATMTASGTPLDAMTGVTAVGSGSMEQRT
                     WGTALQLELTGLPPAAGYRAYVMARNGRTEVAANWGTTPGGRVTINGTTGIPLDQLAS
                     VQIRTLDGRPLMSMRCLESS"
     gene            complement(16755..17282)
                     /locus_tag="VFE39_04160"
     CDS             complement(16755..17282)
                     /locus_tag="VFE39_04160"
                     /inference="COORDINATES: protein motif:HMM:NF007227.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6257826.1"
                     /translation="MTASSSPSHAETFVRALYDDVAGPLFGYVVRLTGDRTRAEEVVQ
                     ETFVRAWRRASELNRGPEELRGWLFTTARNLVTDLWRSDAARPTTVSDERALGAAPAA
                     DDLEQAVQRWTVADALDRLSLEHRAVLVETYYEGRSVTEAAQRLGIPPGTVKSRTYYA
                     LRALRLVLEEMGVAS"
     gene            complement(17380..18309)
                     /locus_tag="VFE39_04165"
     CDS             complement(17380..18309)
                     /locus_tag="VFE39_04165"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET6257827.1"
                     /translation="MKLGLGLPQRTDRYHLRNDVVHIARAAERAGYSSLWVYERVLFP
                     LEPTDGLYGVPGLPWSDGYRTSADPFTVLTLASAVTETVRLGTSVLVAPFHSSLQLAR
                     ALATLDVASGGRVIAGLGCGWSSDEYRGVGADFARRGQYLDEAIDACRALWGPDPVTY
                     RDSRTVIDNALVTPKPPAPLPVLLGGGYTPRAVERIARKADGWLPAGLPASAVAEGWK
                     RIRDRAGEHGRDPERLELIPRANVMFSRAVCGAERQPFHGTLSQVVDDIAALAEAGAT
                     EILIDLTWTARTREELLDLALQVIGELHAGGFR"
     gene            18813..19238
                     /locus_tag="VFE39_04170"
     CDS             18813..19238
                     /locus_tag="VFE39_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6257828.1"
                     /translation="MTTPTRTGDAGRQRWLIVREAFSGITRFADFRAALGVAPDVLSD
                     RLATLVQAGVLEKQPYREPGARVRFSYHLTPAGRELRVVLCALQQWGDERVPHEAGPT
                     IIRRTVDGNRPVRVAFVDDTGGEVPPDGVALIRTAAYPL"
     gene            complement(19294..19482)
                     /locus_tag="VFE39_04175"
     CDS             complement(19294..19482)
                     /locus_tag="VFE39_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257829.1"
                     /translation="MDTDMVAQIGSPKGDPALIAALALDGVQAGAFEVVADEKSKIVR
                     SLLSAELPALHPELTAGS"
     gene            19839..21062
                     /locus_tag="VFE39_04180"
     CDS             19839..21062
                     /locus_tag="VFE39_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET6257830.1"
                     /translation="MDITNESSRRVLVVGLGVSGIATALRLRRIGWTPVLIEKAAARR
                     SGGYFVGLFGAGRAAAQRLGVLDGMADRAASDGANFDIDRAGNRQRGLGFKDLPGLPR
                     LMVRGDVEQAVFAELPADVEIRYSTVPTCIEQDAGGVDVTLTNTADGTTAAERFDLVV
                     GADGLRSTVRSLVFGPHEKYLHRLNYMIAAFDLPGPLSDLARQDGATLLELGRSMWIF
                     PFGNHAPTVLLSYRTDDVDAEFTEPPIERIRAAFGPQPTGRALGEVLQALESADELLF
                     DSVEQVRMDRWHHGRVVLVGDSAWCVTLYAGMGVSAGLAGADLLGTMLERHPEDLELA
                     LTEWEQKLRPYIEYYQQNGISQRAFFTPSSRVQIALRKVLMRGQKLPLTGRLLERMRE
                     SGKASRMKETDIARA"
     gene            21115..21819
                     /locus_tag="VFE39_04185"
     CDS             21115..21819
                     /locus_tag="VFE39_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6257831.1"
                     /translation="MAAVTRHDSGKATRILTVTRELVLRRGVRGVTIAEIAEKAHVGK
                     GTVHLYFGTKEDLVVGLFARDFLAVVERYIQTLTTEPDQVRPQRLCPLLVRTALDHPF
                     VRALQTGDVEMLGILGEHPLSKELLGTLGPGSMMYSLLPVWRRYQLARTDWALDEQAY
                     ALRAIIAGFFEVAAHPHVLPDVTVDFPEQVMARAVAALLGPEQANPADVRAAAQECVR
                     MLADSRAVILASIAPA"
     gene            complement(21859..>22824)
                     /locus_tag="VFE39_04190"
     CDS             complement(21859..>22824)
                     /locus_tag="VFE39_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6257832.1"
                     /translation="VLGGLKVANGSISLGDVQAFIQYSRQFTQPLTQVASMANLLQSG
                     VASAERVFELLDAEEQVPDPAVPVVPSTRRGRVVFEDVSFRYQPDAPLIDDLSLTTEP
                     GQTVAIVGPTGAGKTTLVNLVMRFYELDSGRITLDGVDTATMVRATLRSGVGMVLQDT
                     WLFGGTIRANIGYGDPTASEEQILAAARATYVDRFVRSLPDGYDTVIDDEGSNVSAGE
                     KQLITIARAFLADPTLLILDEATSSVDTRTEVLVQRAMAALRSDRTSFVIAHRLSTIR
                     DADLILVMQSGRIVEQGTHEQLLAAGGAYHDLYSAQFSAAAAELE"
BASE COUNT         3522 a         8129 c         7827 g         3346 t
ORIGIN      
        1 gacgacacca cctgggccag ccccaccgac cagtacgccg acttcgccga attcaccctc
       61 tccaccgact ccccctctcc ccccggccgg acgacgctgc gaagcagttc gtccggcgag
      121 tgcgaaaagg ccggcgactc ctgatggcgt gtcatgacgt gtcggatcgg cagcagcggc
      181 gaccgggcag gtcccccatg atgacgagga ccaccctccg ggccgcgctc ctgatggccg
      241 tgctctgtct tctgggcagc gcctgctcca cgcccacacc gccaccgctg gcccggcgcg
      301 aggtcgcatc ggacacggtg atcgatccgg ctcggccctg cgttcgcgaa gcccccgtgc
      361 cagtcccgtc cccccaggcc gagcccgccg accaggctgg atccggcgga ctcggcgcta
      421 ccttcgacgc gggaacgaac aagatcgtgc tgtccgccgg gcagggcatc accctgcccg
      481 cattgagcca ggcgatcgac gacccccggg ccgttcgcga ggtcgccccc ggcgagtggc
      541 tgctcggcgc gaccatcgac gtactccccg gcgcctccct acggatccgt gcacccgatg
      601 tccgctggct gatgctcagc agcagcccgg ccggattcgt ctccgtcaag gctctcggtg
      661 gcgggctcga catcaccgga acctgcgtca cctcatggga ccccatgcag ggccgcgtcg
      721 acaccgagca cctcgacggg cgcagcttcc tgctcgcccg caacggcggg cagttgacca
      781 tcgaccacgc cgaggtgcgc tacctcggct acggcgaggt ggaagcctac ggaatgtcct
      841 ggcgcgtgca ggggacgagc ggccacatca ccaacagcat cgtgtcgcac ctgttctacg
      901 ggctctacac cttcgaaatc agcggcctcg tcgtgcagga caacgagttc cacgacaacg
      961 tcctgtacgg catcgacccg cacacgaact cacacaatct tctgatcgaa cgcaacatcg
     1021 tgcacgacaa cggcaaacac ggcatcatcc tggccgagga ctgcaccgac agcgtcatcc
     1081 gcgacaacat cgtctaccgc aacgaacacc acggaatcgt gctctacctg aactccgacc
     1141 ggaacctcgt cgaaggcaac aaatccttcg ccaacgccgc taccggcatc aacatcaacc
     1201 agtccgcggg caacaccgtc cggggtaacc acacgtacga caacaccgaa tccggcatcg
     1261 gcgtcagcca gacagcgcag gacaacatcg tcgagaacaa ccagatccgt gggaaccagc
     1321 aggacggcgt ccggctcgtc agcgaggcca ctcacaccac ggtgcgcaac aacatcatcg
     1381 gggagaacgc ccgctacggc gtttacatcg acagcaccgg cccctacgcg ctgaccggca
     1441 acaccatctt caacaaccga accggcatca tgttcaagaa cagaaccacc actcccccga
     1501 ccgacaacca ggtcttcgcc aacgacaaag ccgacatcgc gaacaccgag agccgctgac
     1561 gggccggaca gctcgcggtc ctcgccgggc gaggttcacg ccacggcgcg gccggcgtgc
     1621 ggtcaggtca ccccgccatc agacggcgca ggaacggggc caacagggcg agcacgaggc
     1681 ccagcgcgat cgcgacggca ccgagcaccc cgaagtaggc agcctcggcg gtggggctgt
     1741 agtactgcgc caacaccccc gacattgctg tgcccagcgc gatcgacagg aagaacagag
     1801 ccaccatctg ggtgcggaac gcctcgggag cgagtttcgt ggacagtgac aggccgaccg
     1861 gcgacaacag cagctccgcg atggtgaaga cgaagaggat gccgatcagc ccgagcaggg
     1921 gagcgctgcc gggatcgtcg cctgccatcg gcaggaacag caggaacgcc acgcccatca
     1981 tggcggttcc gagcgcgaac ttgatcgggc tcgacggctg ccgggacccg agcttggtcc
     2041 acagcgcggc gaacgcaccg gagagcagga tgacgaacac ggggttgatg gactggacga
     2101 aggagaccgg catctcccag ccgaagatgt cccggttcag ctgctcgtcg gagtagatcg
     2161 tcaccaccgt gaactgctgc tggtagagcg accagaatgc cgcgctggcg atgaacatcg
     2221 ggatgaaggc gagcacccga cgccgctcca gcacggtgat ccgcctgctc cggagaatga
     2281 tcacgaagta gacgatcgtc gcgatcacgc tgagggcgac gacgacgttc gcgaggcggt
     2341 ccggcgtgag caccccggtg aacgccagca cggcgatcag ggccacagca gcgacagccg
     2401 tccccgcgac caggggccac cgggacgccg gcagcgggct cggcacctcg cgggccttcc
     2461 cggtcaggtg cttgcgaccg atgctgtact gcgccagccc gatggccatg ccgacggcgg
     2521 cgagcccgaa gccgtagtgg aacccgaggc cgctctggag cagcccggtg agaatcggcc
     2581 cgacgagtgc cccgaggttg atcccgaggt agtacagcga gaagcccgcg tcgcgccgca
     2641 cgtcccccgg tgcgtacagc gtcccgacca gcgacgtggc cgtggccttc acgccaccgc
     2701 tgccgagcgc gatcagcacc agcccgacgc ccacacccag caggccgggc agcaccgaca
     2761 gggccaggtg gccgatcatc accaggaccg cgctgtagaa gagcgtccgt tcggcgccga
     2821 gcatgcggtc ggcgagccat gccccgagga tggtcgacag gtacaccaac ccgccgtagg
     2881 cgccgacgat gctggtggct gccgtctggt cgatccccag cccaccctga gctgtggagt
     2941 agtagaggta gatcagcagg attccctgca tcccgtagaa cgagaagcgc tcccacatct
     3001 cgacaccgaa caggttcgcc agaacccgcg gctgaccgaa gaacgtcttc tcggtacgag
     3061 ctgcatcggc cacatctgtc acgacgcacc atccgctccg tagggcgcgg cacaggtccg
     3121 gaacggaacg tcccaggatt cgcgaccttg cgattgccgt cgactgtatt ccctgcgacc
     3181 ggatgggaaa aggtcacgac cggctgacgc ggtggtggtg cgtgacagta cgggtgactc
     3241 cgtggcgaaa ccggatgttt ttcctcccgg gcgagaagtc tgccaccatt tggccgtatc
     3301 gtcagcgcgg tgaccggccg ccacgactcg tccattcccg cgccggacgt tgctgctctc
     3361 gtggcgtcct gggccggcta tctattgcgc ccggggtacc tgccgatgtc gcgggaggag
     3421 ttgcaccagt tcctcaccgg ggcggcactg cggttggcgc acgccgcggc ggccgagccg
     3481 gttgatctcg ccgcggcccg gcgggtgggc gcctcgatcg tccacggcgg actgcactct
     3541 gccgatgtcc tgccggccac agtggccgtg ctggcccggc atctcgtccc cgtggcgcag
     3601 tcggcaggcg ccgaccggcc acaggagacc gcgccggcca ttcaggcggc tgtcaccgac
     3661 ggctacctgg cacagctccg ggcccggatc cgggacgagc aggagatgac gcgcagagcg
     3721 gaggtcacgg cccgccgcca gatcgagggg gctctgcgcg ccagcgaagc ccgcttccac
     3781 gccatcttcg ccaacgcggg gatcggcatt ggcatcgcgg acatggaggg ccgcatcgtc
     3841 gaggtgaaca cggcgttcgc cgggatgctc ggatacacgg tcgacgagtt ccgccggctc
     3901 acggtgggcg acctggtgta tcccgacgac gcaagcggaa ggtgggacct ctaccgggac
     3961 atcgtcgagg gcaagcggca cagcgcccgg gtcgagaagc gctaccggca tcgcgacggg
     4021 cacgctgtgt ggaccgacct caccgcctcg ctcatccggg acgcccaggg cacccccctc
     4081 tacaccgtcg cgatggccga ggacgccacc acccgccgcg acctgcagga gcggttgcag
     4141 agacaggccc tgcacgaccc gttgaccttg ctgcccaacc gcgccctgtt ccaggagcgg
     4201 ctcggcgcgg cgttctcccg cccgggcagt cacgtcggga tctgctatct cgacctggac
     4261 cggttcaagg ccgtgaacga ccggctcggc cacgacatcg gtgacgcgct gctgatcgcg
     4321 gtggcgcggc tgctggacaa gtacgtgtcc acccgtggac atcttgtcgc ccggatgggc
     4381 ggcgacgagt tcgtcatcct cgtcgaccac ccaccgaacg gcgagctggc gatcctcgcc
     4441 gacaccatcc tcgccgtgct caccgacccg atcgacgtcg acgaacaccg catcgtcgtc
     4501 tcggccagca tcggcgtcgt cgagtgcggg gtcgacgaga cgacgcccgc ggagatcctg
     4561 aaggccgccg acgtcacgtt gtactgggcg aagtccgagg gccggaaccg gtgggcccgc
     4621 ttcgaccccg agcgcaacgc ccgggacatg acccgctaca cgctctcggc caccctgctc
     4681 cccggcctgg aacgcgacga gttccgggtg gagtaccagc cgatcgtcga gctcgtcgac
     4741 ggccgggtgc gtggcgtcga ggcgctcgtc cgctgggtac accccaccct cggccggctc
     4801 agccctgacc agttcatcga cctcgccgag gagaccggca gcatcgtgcc gctcggccgg
     4861 cgggtgctga ccgaggcctg cgagcgtgga gccgagtgga acgccgcgca ccccgaggcc
     4921 gagctgttcg tcagcgtcaa cctggccgtc cggcaggcac acgaccccga cctcgtcgac
     4981 gacgtcgccc gcgccctcgc gagaaccgga ctgccgccgc atctgcttca gctcgagctg
     5041 accgagagcg cgctgctcgg gccggccggg agacccgtcg aggcgatcac ggcactggcc
     5101 gccatgggag tccggatcgc cgtcgacgac ttcggcaccg gctattccaa cctcgggtac
     5161 ctcccccggc tccccctcca cactctgaag ctcgccggaa tgttcatcga gggcctgcgc
     5221 aaccccgcaa ccggggccga ccccatcgtg ggtagcctga ttgccctcgc ccacgcactc
     5281 ggcctcgtcg tcacggccga aggggtcgag acgcaggccc aggccgaccg gctccgcgcc
     5341 ggcgcctgcg acacggccca gggatggctc tactcccggc ccgcatcctg ggacgacctg
     5401 gtccacctgc tcgaccgaca cctcccgccg gggagctgat cgaggacagg cagtcaccac
     5461 cctggtgcac cttggctacg gggggccggt ggtggttcca tcgtcactcg tcccacgcga
     5521 cggaacggca cggcccacga gagaccttcg gtgagtaacc gaggtcgtgc gcccgcgtac
     5581 ggggacggct ggtcggcgga aatcttcgat cagatcaggg cctgaccgac agtcccggtg
     5641 ctgacggaga cggaggcatg gacatgagcc acggtagcgg tcggatcgtc gtcggagtgg
     5701 acggctcgtc cggtggacgc gcggcgctgg cgtttgcaat gcaggacgcc gcccggcggg
     5761 acgccgcagt cgaggaggtc gtggcgttcg ccaccgccga gaccctgtcg gcgctctgcg
     5821 accctccgta ccgatcgttc accacctcgg cggccgaggt gcgcgccgcc gtcgagaaga
     5881 aggccgcggg catcgtggag gacgtgagcg gtgagctggc cggcgagctc gagagcctgc
     5941 ctccggtggc cgtcacggct gtcggtggag gcgccgttgt cgcgctgctc cgcgccgccc
     6001 acgatgccga tctcctggtg gtcggcagcc ggggccgcgg tggcttcgcc agcatggttc
     6061 tcggctcggt cagcatgcag tgcgtgctgc acgcgtcctg cccggtcacc gtcgtgcacc
     6121 ccgttgcggc actgcccccg gtggcacgtc gtggactgca gctagagcgc gctcgtcgcg
     6181 ggggcggtca ggcgccggcg actctcacaa ccgatccgga gacgcggtcg aggcggtgga
     6241 tccggaagaa cgtccacatc agggtggcgg cccggggaac ggcctggtcg ctgtaggagc
     6301 cggcggggcg cccacctgca cgtaccgccg ctccggcacg ctgcccaggg ggtgggcagc
     6361 tgacggcggg ccccgatcgg atcaggacgg cgtcacgtca ggacgtaggt gccgggcccg
     6421 tcgccgagca ccgggtactt cgcgctgccc atgcgcggcg gttcctgcag cggacccgag
     6481 ttggcatccg accagcgcga ccagtcctcc caccaggagc cgctgcgacg ctcggccacc
     6541 tcgcgccacc cgtctccggt ggccggcgtc gtctcggtct cggcgaccat gtgccagacc
     6601 ttcggtcccg gcgggctgac gatgccggcg atgtgccccc cgctgctcag gacgaaccgg
     6661 accgggccac tgatgaggcc gacgttcttg tacgccgatt tccacggcac gatgtggtcg
     6721 ttgatggcgc tgacgacgta ggtcggtgcc ttgatggcgc tgaggtcgat cgtcttcccc
     6781 gcgatctcga gctcgccggc cgcgagcttg ttctccacgt agaagttgcg caggtagaac
     6841 gcgtgcatcg cggccggcat gcgggtgctg tcggtgttcc aggcgaggat gtcgaacgcc
     6901 ggcgggtcct ggcccatcag ccagttcgac acgacgtagt tgaagatcag gtcgttggcg
     6961 cgcagcgcgt cgaacgtgcc ggccatcgac ttgccctcca gcatgccctc ccgtcgcatc
     7021 ttcttctcga gcttctcgac cgtgcgcatg tcggtgaagg tgcccagcac gcccggctcg
     7081 gcgtagtcga gcatggtgtt gagcagcgtc agggaaccga tgcggtggtc gcccgcctgc
     7141 gtcaggtacg ccgcggtgat cgcggtcaac gctccgccga ggcagagccc gacgatgtcg
     7201 atcgtcttgg cgccggtgat ctcctcgatg acatcgagcg cggtctgcgg tccgtggacc
     7261 aggtagtcgt ccatcgtggt gtcggacatg gacttcgacg ggttcttgta gctgatcgcg
     7321 aagaccgtgc gcccgtggtc caccgcccac tggatgaagc tgcggtcttc ggcgaggtcc
     7381 atcacgtagt acttgttgat ccacggcggg ctgcacagca gcggcgtggc gtgcacgtgc
     7441 tcggtccgcg gctcgtactg cagcagctcc atgaggtcgt tgcggtacac gaccttcgcg
     7501 ggcgtacggg cgaggttgcg gccgacctcg aacccgctgg tgtcgacctg gcgcggcctg
     7561 ccctcgttgt tgaccaggtc gtcgaggaag ttccgcgtgc ccttcagcag gctcccgcct
     7621 gctgtgtcga acgcgcgctt cagcgcagcc gggttggtcg cgaggaagtt cgtgggggcg
     7681 agcgcgtcga gcatcaggtt catggccatc gcggccttgc gggcctcgtc ggcctccagc
     7741 tccgccgagc cgacgacgtc gcgggcgaac cggcatgcgg cgaggtaggc ctgccgaacc
     7801 gagtagaaca tcgggttcgt gtcccacgcg gggtcggcga accggcggtc cttcgggtcg
     7861 acgggcaacg gcggctcggc gtccccgccc agccagcggg tggtagcgac gaacgggatc
     7921 cgcgccaggt ccgtcgcgag gttgatcgca gccaacgcgg gagcgcccgg gctggcgagg
     7981 ctcgctcgca ccgcctgggt cagcgcatcg ccgaacccgg cggcgtccat gtcctcgaac
     8041 aggtccccct ctggtcccag cacgctcgcg gcgcgctcgg cgacgtcccg gggcgtttcg
     8101 ggctgttcct gggccatgca ccccatcctt gcgctacgag ggtccgtggc tgcgcttctc
     8161 ccgctggctg gaaccgagtt gatgccgctg cagcacagtg gggcgaacag ctaccggtca
     8221 tcggacacgt cgggcaccgc gaccacgggt cgtcgcgcac gcttgagcac gtcacgggtc
     8281 gtgctgccca gcaggaactc gctcagcgtc cggtgccggc gagaccccag gacgatggcg
     8341 gacgcgtccc gctcgtcggc gacccgcagc agcgtcctcg ggacacccgc atcgtcggcg
     8401 acgacccggc cctcggcctc gaggccggcc tcccgtgcca tcgctgcgcc cttctccgcg
     8461 agctggcgag ctgtcgtcat cgccttctcc tcgtactcga gggcggcctg tatctcgacc
     8521 tccgtgagcg gtatccccgc ccccgccaac ggccccacga ccgcggcgaa cgcttccccc
     8581 gggacgagca cgaccagcac gacggcggga cgcggggcga acagccttcc cgcctcccac
     8641 acagcccgct cggcagccga cgtaccgtcg aacccgatga ggatcggtcc ggatggcatc
     8701 tggccctcct tcgggacggt cctcctccgc tgtggccgag gcgcgttgcc acggtggtcg
     8761 gccaccggcg gccgcgcttc ccccctcggg ggccaactcc atggcgaccg ctcgacctgc
     8821 gggggcgacc gcgtccgacc gtcgtccact ggccgcgaac cggcgccctg cccgtgccat
     8881 gacccgcggc accgacgagg ctgagcgtgg agccgaccac gggggcgacg atctccgtgt
     8941 ctgctcggcc cgctgccacg gcccaccgct cccccgggcg cacgcaccgc gctcgcaacg
     9001 gtgtgcagct ccgaaacggg cacggaggcg gtcgagcacc gccgtgatgg cacagcgggg
     9061 ctcgaccgcg tcggggctcg cggtcaggtc ttgagctgct ggcgcatggc gctgaaaagc
     9121 acctccgacg tatctttgac gaagttggcg tgggcggtcg ccagcgtgct ggcccactcc
     9181 gcgcccgtgc ccttggcagc ctgctcctcc aggtcgagca ggttcttgag cattcgctcg
     9241 tagccctcca tccatgcgag gccgttcttc ttggcctgct cgacgacctg ctcactgagc
     9301 tcgcggacgc gctgggtagc ggcctcgagg tcgcgcgtgg ggtcgggtgt gctcatgtcc
     9361 gtgactcctc tcctcaggtg gactgcgtat cccattccgc cacacccatg cccgtgatcc
     9421 atggatatgt cggctcgtac gcgagggtgg tgggggagcg ggagccctgg ccgcgcgcct
     9481 tccggatcgc cggcctggcg cgcggtcccc ggccgccccc ggcagcatcg gccgccgcct
     9541 cctcgtgctc ggcctcgctc gtgacctcag gtctcggccg acatcagccc gagcagctcc
     9601 acccgccagt cggcgtagga cccaccggag gcctcggccg cgagcgactc ccactccggg
     9661 tttcggccca tgccggtgag cgcgatctcc ttcggggtcg agccggagcc cacgacggag
     9721 acccgcagac ccgcaggccc gtcggcccga gccgcatggc ccaggacgcg gacgagcgcg
     9781 gcccgggccg acacgtcgtc gtccgtcgag acctccggcg gcagcacgcc catcacgaac
     9841 gtgatccggg cctccggcgt gagtgcgggc aacgccgcgg agacggcggg gaaccgggcc
     9901 agcacaccgt cggcgaagaa atggtgcacc cgctcgacgg ccgtgtcgcc ctgcaccatg
     9961 aatgtcgcgg gcagttggat gtacccgtcg aacgcacgat cccccgcatc ggaacaggcc
    10021 cgcggcacgt cgccgatgtc gtccacctcg accaccgtgc tgccgttctc gcgcagcacg
    10081 gtggcgacct gcgcaacgag ctcggcgatg ccggagacca ggaagtgcgg catggggttc
    10141 ctctcgtcgt gggcgtcgat cacgggcggc gcgtcggcgc gtcgcccgtg agcttggcgg
    10201 aggtgtcgat gccgtcctgc tcgacggtgc gccagctgct cgtcggttcg cccatcgctc
    10261 cgcctcgtcg tcggttcggg tgaccggcgc cgagtcaagc accggccacc agcccacaag
    10321 ggacaccgaa gcgggtttcc tgctcagtgg gagtcccgcc gatcaggccg atccgcgttg
    10381 gacgggcgcc gggacgggcc cgacccccgc gtacggccgg acgagcggag ccagctcacg
    10441 tgacagccac cccgccgcca cgacgagggg ggcccgcagc gccggtacgt ccctggcgag
    10501 gtcccgcacc cgcagctcca cggcgaccac gacgtctccg cccgccccga acgccggggc
    10561 ggcgacaccg caccagtcct ggtcgagctc gcggtcgcac accgcgatcc gcgcggcgcg
    10621 gatcgtccgg aagctccatc gcagccgctc cgcccgcgtc accgtggatg tcgtgtaccg
    10681 gacgagctcc cgggagagga gcgtgtcgac cgtccgcggc gaggagaacg cgagcagcac
    10741 cttgccgagc gccgttgcgt gcgccgggag acgcgcggcg ggagagggca acgacaccgg
    10801 gaggtgccct gccgccttct cgacgtaggc caccgccgcc ccgtcgagga accccacccg
    10861 caccggagcg cccgtcaccc ggaagagatc ctcgatcacg ggcacggcgc ggtccctgat
    10921 gcgcgcggcc ggcgtggccg cgccgtcgtc gggctcgtgg cccgaacagg cgctgccggc
    10981 accgatcgtc cgcaacgggg gcccggcccg gtaccgcccc tccccgtcac gctccagcac
    11041 tcgccacgcc gcgagctcgg tgaccagccg gtgcaccgtc gacaacggta ggtccgaccg
    11101 ggcggcgatt tccgtgagcg tgggagcgct gccctccgag acggcgagca gaatcgcgct
    11161 gatcttgctg atcaccgacc tgccggggtc cttcgatcca cccgccacgc ggcctcctct
    11221 cggctggagc tacgtcaggc tcaggggacg tcgatggtca cggcagccgc gtgattcatg
    11281 atcaatgtgc ggtcgtcgga ctagcagggg atcaggggcc ggtgcgggtg cggtccgcat
    11341 aactcacgac ccacccgagc agcaacaccg accacagcgg ccgatacggc accaatcggg
    11401 gtcgcgtcac ggcccgcctc cagcagcccc tcgttccgcg cggtgccgcc ctcgtgcgcc
    11461 cgttcgccat tgcccctcct cgcggagatc cgtcgtcgtt gccggatgag tggccgctcc
    11521 cctgtgcgtg ggagggccct cggatgcacc acgaatggtg cgtccgtgca gtacggattg
    11581 ccagcgggat cacacgcgaa acgtccggga ggaatacggc cgggagaaca gggggcatcg
    11641 cggggtcggg ttaggcgctc agggcggtcc gcaccaagcg caccgggagt gcaccgcgac
    11701 ggcatggccc gcatcccgcg ggacctcgcc gtaggccatg tagccgccgc ccatgccgac
    11761 ctcctgcacc tgcatgccgc cgatccgctc ggccaggccg gtccagggcc ggtcgaagcc
    11821 ggtgaagtcg cggacgtagg gcgtgtgcag ggcgagcacg gcggcgttga ccgccatcgc
    11881 ccacgcgggt gcccacttgc caccgcccgc ggccacgccg ccgtgctcgc gcacgtgggc
    11941 gagcacgtgg tccagcgcag gtccggactg gaggacgtcg tgcaccgcgc agaacagcgc
    12001 gtggtcggcg ccgtcgggca ggtcggcgtg ctcgacggcc gcctcgacca gcaccacgtt
    12061 cgcccaccgc tgcgaggtca cccgctcggc agcgatcgtg agcatgtccg ccgacgtgtc
    12121 gatgccgacg atcgtgccgc cgggcccgat gtgctcctgg acctgcgcga agcacaggcc
    12181 ggtgccacag ccgatgtcga gcacgacgtc gttccgctcc aggggcaaca ggtcgacgat
    12241 ctgccggcga tagcggtcga agggcccggt gcgggcgtcg taggtggcgg cgtcccggct
    12301 gtaggcgggc agcgtgggct gtgtggcgcg ctgcgccgga cccggggcgg atggcagggc
    12361 tgtgggacgg gccatgaagt ggtttccctc ggcggcgggc tgccggatcc cggcagcaac
    12421 ggggccgggc gcaagcatcg cggcgcgtcc ggctttgcgg acaccacgac ggtgtgaccc
    12481 tcccatagtg gtgccggaca cgggccgggc ggaaccccgt cggggccgtc tcgggcctgc
    12541 gcccacggcg accatccctt gaccatccca gtggatggca gcaggatggt ggcgtggccg
    12601 aggtcgacaa gagcgagaag atcaccatca atgtggggct cgtcgacctc gggcagatcg
    12661 acctgctcgt ggacgaaggc ttcttcgcca accgcaccga cttcatccgc accgccatcc
    12721 ggcgccagct cgagacccgc tcggacgcga tgacagacac gatcgggagg cgagcgctca
    12781 ccctcgggac gaggcacctc gcccggcacg agctcgagca gctccgcgat gccggccgca
    12841 cgaccgagct ccgcgtcctg gggctcgcga ccatcgccga cgacgtcgac gtcgagcttg
    12901 cgctggcgac catcgccgcg gtcgaggtcc tcggggcgtt ccgtgccccg cgggacatca
    12961 aggccgcgct cgccccgcgc accgtctgac ggcagccagc agccgcccgc gccgtcgcac
    13021 cgcacggcat tcccgcccag gaggaccgtc atggatcacg acccgaacat cggcatggca
    13081 tcggcgctgc agctcacctg ggcaggacgg ctgaccgagg cggtcgcggt catccagcgc
    13141 accctgggcg ccgacggcac gcgggtcgtg cgcggatacg ggtgctccag cactcgtatc
    13201 cacgcacagg ggggcgggca gccggtgcac ctgacccaca ccgaagcggc cggcacccgc
    13261 cgcttcgacc tgtacgttcc ccccggccgc accggtgagc gtctcccgct cgtggtcatg
    13321 ctgcacgggg gttcgcagaa cgccgctgac ttcgcggccg gcacgcggat gaccgagctc
    13381 gcccagcagc acggcctgct cgtggcgtat cccgagcagt ccagggcagt caaccggggc
    13441 ggctactgga actggttctc ccccgcccac cagcgcgcgg gggcgggcga gccgtccatc
    13501 ctggccggga tcagcaggcg gatcgtcgcc gagtacggcg ccgacccgag ccgggtatac
    13561 gtcgcggggc tctccgcggg tggcgccatg gccgcggtgg tggccgcgac ccatcccgag
    13621 ctctacgcgg cagtgggcgt gcattcgggc atcgcggccg gtgcggcgga cgacgtgggc
    13681 tccgccttcg ccgccatgcg gacgggcggc acgccgggcg cgggcgccga cctcccgctg
    13741 atcgttttcc acggtgacgc ggacaccacc gtggctccgg tcaatgcgga gcgcctgatc
    13801 gacgcacgcc tctccgccgg ctcccggcgc accaccgccg cggcggtccg actcgccgaa
    13861 cccggccgcc ggccgtacac gcgcaccgtg atccgcgacc aggacgattc ggtcgtcgcc
    13921 gagtcgtggc tcgtccacgg gatgggccac gcgtggtcgg gcggcgaccc ggccggctcg
    13981 tacgcggacc cgaagggccc agatgcgtcg gccgagatgc tccgcttctt cctcgagtac
    14041 gcctaggcct acccggtggg ggtcacggct tgcggccgac tccacccgcg tagaacgtgt
    14101 gccagggact gtccgccggg gtgtggccgt ccggacgcca gtcgttcgca taggtcaggc
    14161 ctggctccac catctcgagg ccgtcgaagt agcgccggtg ctcgtcccag gtgcggaacc
    14221 gcccggtgcc gaagccgccg tcgatgaacg cgcgttccgc ggccttggcc cgagggtcgt
    14281 cgtcgtcgag gaagttgctc gccagcaggt agctcccgga gggcatccgg tccctgaagg
    14341 tacgcgtgac cccgaagggg tcctggtcgt cgttcaggtg atgcaggatc cccgacagca
    14401 gcaccgcgaa cggcttgtcg aagtcgatga actcctgggt ctccggggcc tcgaggatcg
    14461 acaccgggtc ggccgcgttg gcctggatca ccgttgtggt gttgttgtcg gcgagcagcg
    14521 cgcgggcgtg ggcgagaacg atcgggtcga cgtcgacata gacgacatgc acggcggggt
    14581 cgagctcgtg cgcgacctcg tgcacgttgc ccgcactggg caggcccgat ccgacgtcga
    14641 tgagctgccg gatcccggcc tcaccgacga ggtaccgcac gccgcggcgc aggaagcccc
    14701 ggttgtcgcg ggccagcggg agagcgccgg cgaaggcatt ggtgagggca tccaccgcga
    14761 gacggtcgat ctcgaagttg ttcttgccgc cgagcgcgaa gtcataaccc cgcgccaccg
    14821 acggtcgcgt gatgtcgatg tccggaggga ccgcgggagc ctcgcgcacg gagtcctgca
    14881 gctgtctttc cgactcgctc acttaaccct cgttctcttc ccgggtggca tcgtccgcga
    14941 cgtcctacat taggggaaga tcatcgatca gcgcgccctg gcctgatcac ttgttgatcg
    15001 ctgtccgaac ccggcgcacc gcgatcaggt gatcggcgac ccgccggtca caccgatcac
    15061 ctcggaggtg atgtagctgg attcctggga ggcgaggaac acgtagatcg gcgcgagctc
    15121 ggccggctgc gcagcgcggc ccaacggcga ctgcttcccg aacgacttca ccttctcctc
    15181 cgacatggtc gccgggatca gcggcgtcca cacggggccg ggcgccaccg agttcgcccg
    15241 gatgcccttc tcggtgaggt tgttccccag ccccttggtg aagttgacga tcgcggcctt
    15301 cgacgtcgcg tagtcgagga ggtgcggcga agggttgaac gcctggacgg acgaggtcgt
    15361 gataatcgac gagccctccg gcatgtgcgg gacggccgcc ttgcacaccc agaacgtcgc
    15421 gtacacgttc gtcctcatca cccggtcgaa ctgctcggtg gtgatggcct cgatgccggc
    15481 gtcctgcgac atctggtagg ccgcgttgct caccagtacg tcgatccgcc cgaactcctg
    15541 caccgcccgg tcgatcacgg tctggcagtg tgcctcctcc cggaggtcgc cgggcaccgt
    15601 caccgccgtg cggcctgcct cctccacgag ccgcaccgtc tcctgagcgt ccttctcctc
    15661 cgactccaga taggagatga gcacgtcggc tccctcgcgg gcgaacgcga gcgccacggc
    15721 gcggccgatc ccggagtcgc ccccggtgat cacggccttc ttgccctcca ggcgtcctgt
    15781 tccccggtag gtctcctcgc cgtggtcggg ccgaacgtcc agctcgcccg tgcggcccgg
    15841 gtacgccacg gtctcgctgc cgctgcccgg ggcggcgtgc tgctcgccgg ggtcctgctg
    15901 ggtgaactgg tcctggtcct ggcccatcgt gtcactccgt tcgcgtcgcg gtactggcgc
    15961 aaacgttccc cgaaaagacg gtcgcaaccc tcgtggggag atcacgtccg ggcgtgagaa
    16021 ccacacggtc ccggaaacag ttcaggaaga ctccaagcag cgcatgctca tcagcggacg
    16081 gccgtcgaga gtgcggatct ggacggaggc cagttggtcg aggggaatac cggtggtgcc
    16141 gttgatggtg acgcgtccgc ctggggtggt cccccagttg gcggcgacct cggtgcggcc
    16201 gttgcgagcc atcacgtagg cccggtagcc cgcagccggc ggcagcccgg tcagctcgag
    16261 ctgcagtgcg gtgccccacg tacgttgctc catgcttccc gaacccaccg cggtgacgcc
    16321 ggtcatggcg tcgagcgggg tgcccgaggc ggtcatggtg gcgggggtcc cggaggcggt
    16381 cacagtggcg ggcgggggcc cggaagcgcc ggtcgggagc acctgaggaa gcacgcccga
    16441 catgccggcc acgaccgcag cggccgtggc taccagcttc caccggcgca gcctgcgccg
    16501 gtcctgttgc tggtggtgtc ggacagcacc gagggcctgc ggcaggagag cggggctcgg
    16561 cgtcagcatc ccgttgcggg cctctgcagg ctcgagacaa cccagcagcc cggcgacagg
    16621 cgcgagcatc gccagctcgg atcggcactc cgcgcagtcc gcaagatggt ggtcgaccgc
    16681 atcgcgctcg gggggctcga ggctgcccag gacgtaggca ccgagctcgg tccgcatgca
    16741 cttgtcctgc tcggtcacga cgccaccccc atctcttcca gcaccagccg caacgcgcgg
    16801 agggcgtagt acgtgcggga cttcacggtg ccgggcggaa tgccgagccg ttgcgcggcc
    16861 tcggtcaccg accgtccctc gtagtacgtc tccacgagga ccgcgcggtg ctcgaggctg
    16921 agccggtcga gtgcgtcggc caccgtccac cgctgcacag cttgttcgag gtcgtcggca
    16981 gctggtgccg cgcccagggc acgctcgtcg gagaccgtcg tcggccgcgc ggcatcggac
    17041 cgccagagat cggtcacaag gttacgcgct gtagtgaaga gccagcctcg cagctcctcg
    17101 gggccacggt tgagctcgct ggcgcggcgc cacgcgcgga cgaatgtctc ctgcacgact
    17161 tcctcggctc gggtccgatc gccggtcaac cgcacgacgt agccgaagag tggcccggcg
    17221 acatcgtcat acagcgcccg cacgaacgtc tccgcatgag aggggctgct cgatgcggtc
    17281 acactcagtg caacgcgtcc cggatccagc tagttcatcc gggaccccgc ctgtgactga
    17341 gaccatgagg acgcggatca accgacaaac gggtgttgat cagcgaaaac cgccggcatg
    17401 gagctcgccg atcacctgta gcgccaggtc gaggagctcc tcgcgggtcc tggcggtcca
    17461 ggtgaggtcg atcaggatct cggtggcacc cgcctcggcg agcgcggcga tgtcgtcgac
    17521 cacctgcgac agcgtgccgt ggaacggttg ccgctccgca ccgcacacgg cgcggctgaa
    17581 catgacgttc gcccgcggga tcagttcgag acgctcaggg tcgcgaccgt gctcaccggc
    17641 gcgatcgcgg atcctcttcc agccctccgc caccgccgac gcgggcagcc ccgcgggcag
    17701 ccagccgtcg gccttgcggg cgatccgctc cactgcgcgc ggcgtgtacc caccgcccag
    17761 cagcacgggc agcggtgccg gaggcttcgg cgtcacgagc gcgttgtcga tcaccgtgcg
    17821 tgagtcgcgg taggtcaccg ggtcggggcc ccacagcgcg cggcaggcgt cgatcgcctc
    17881 gtcgaggtac tggccgcggc gcgcgaagtc cgcgcccacc ccgcggtact cgtcgctcga
    17941 ccagccgcag ccgagaccgg cgatcacgcg gccgccactg gccacgtcca gggtggccag
    18001 cgctcgcgcg agctgcaggg acgaatgaaa cggcgccacc aggacgctgg tgcccagccg
    18061 caccgtctcc gtcaccgccg aggcgagcgt gaggacggtg aacgggtcgg cggacgtgcg
    18121 gtagccatcg gaccacggga gccccggcac gccgtacaga ccgtcggtgg gctcgagcgg
    18181 gaacagcacg cgttcgtaga cccagaggct cgaatagccc gcacgctccg ctgcccgcgc
    18241 gatatgcacg acgtcgttgc gcaggtggta cctatcggtg cgctgtggaa gacccagccc
    18301 gagcttcact gtccctgctt tcctagcaat tggccgaccc gacgtcgcgt ggtcctgtcg
    18361 acgcttcgga ccatcgatag aaggcagccg cctgtcccac ccgcaccccg gcaccgttca
    18421 cgagattcca catcgtcacg tcctcgatcc agaaccgccg accggacttg gcgatgcgca
    18481 acccgcggta cccggtggca aaatcgttcc gctggacggg gtcgagcagg cagcggaggg
    18541 tgggacaccg actcacctcc tcgttcgtca ggagggtcat gcgccgagcg atcgacgatc
    18601 atcacggcgt tggtgagctc tgagggcctg acccggtgcg cgaccgtgct cggctggggc
    18661 cgccacggcg cgtccgacgc ggggatggcg cacagctcat cctagacgtg catcgtgtcc
    18721 catcatcgtt gccctccacg gcgagaacgg cccggtggtg gtgacagttc ccgcaacggt
    18781 gtgcacatga cggacctcct ggtcgacggg gcgtgacgac cccgacccgc acgggggacg
    18841 cagggcgaca acgctggctg atcgtccgtg aggcgttctc cggcatcacc cggttcgccg
    18901 acttccgcgc cgcgctgggc gtggccccgg acgtgctcag cgaccggctg gccacactcg
    18961 tccaggctgg cgtgctggag aagcagccct accgcgagcc cggggcacgc gtccggttca
    19021 gctaccacct cacgccggcg ggccgggagc tgcgcgtcgt gctctgcgcc ctccagcagt
    19081 ggggcgacga gcgcgtgccc cacgaggcgg gtcccaccat cattcgccgc acggtcgacg
    19141 gcaaccgccc ggtgcgggtg gcattcgtcg acgacaccgg cggcgaggtg ccgcccgatg
    19201 gcgtcgcgtt gatccgcacc gccgcctacc cgttgtgacg cccactgatc aacgaggccg
    19261 agctgggaac aagcaccgag ccgcagccct cggtcaggac ccggcggtca gctccgggtg
    19321 gagggccggg agctccgcgg agaggaggct gcgcacgatc ttgctcttct cgtccgccac
    19381 cacctcgaaa gcgcccgcct gcacgccgtc cagcgcgagg gcggcgatca acgccggatc
    19441 gcccttcggg gagccgatct gcgcaaccat gtcggtgtcc atgtagccca cgtacagggc
    19501 agtcgtcggg atgcccttcg gcgcgagctg cagggcgcag tgtgttggtc atcgcccagc
    19561 tcgcggccgt ggccgaggag tacgccccgt ggtacggggt gtgcacccag gcaggatgga
    19621 caggagttca gcaccccgcc gcccccgttg cgctcgatga tgggcgcgta cgcacgcgta
    19681 cggcagcctc ccatcggcgc cggttcacac cgtccacgat ggacgcagtc agtcggccgc
    19741 cccgagcgcg agccccgcca cgacatcgaa ctagagtgtg acaagcgcca taaactgacc
    19801 gaatgacgga tacagtcaac tcggcctagg ctctgatcat ggacatcacg aacgagagca
    19861 gtcgccgtgt tctggtggtc ggtcttggcg tcagcgggat tgccacggcc ctgcggctgc
    19921 ggcgcatcgg ctggaccccg gttctcattg agaaggccgc ggcgcggcgc tctggcggct
    19981 acttcgtcgg cctgttcggg gcgggtaggg cggccgcgca gcggctagga gttctggatg
    20041 ggatggccga ccgggccgcc tccgacgggg ccaacttcga catcgaccgc gccgggaacc
    20101 ggcagcgtgg cctgggcttc aaggacttgc cggggctgcc gcggctgatg gtgcgtggcg
    20161 acgtcgagca ggcggtcttc gccgagctgc cagcggatgt ggagatccgc tactcgacgg
    20221 tgccgacctg catcgagcag gacgccggcg gcgtggacgt gacgctgacg aacactgcgg
    20281 acggcaccac ggcggccgag cggttcgacc tggtcgtcgg cgccgacggg ctacgctcca
    20341 cggtgcgatc gctggtgttc ggaccgcacg agaagtacct gcaccggctc aactacatga
    20401 tcgccgcctt cgacttgccg ggtccgctga gcgacctcgc tcggcaggac ggcgcgaccc
    20461 tgctcgaact cgggcggtcg atgtggatct tcccgttcgg caaccacgct ccgacggtgc
    20521 tgctgtccta ccgcaccgac gacgtggacg cggagttcac cgagcccccg atcgagcgga
    20581 tccgcgccgc gttcggcccc cagccgaccg gccgcgcact cggcgaggtc ctccaggcgc
    20641 tggagtcggc cgacgagctg ctgttcgact cggtggagca ggtacgcatg gaccgctggc
    20701 accacggccg ggttgtactg gtcggcgact cggcatggtg cgtgaccctg tacgccggaa
    20761 tgggcgtctc ggcgggcctg gcgggggcgg atctgctcgg cacgatgctc gaacgccacc
    20821 cggaggacct ggagctcgcg ctgaccgagt gggagcagaa gcttcggccc tatatcgagt
    20881 actaccagca gaacgggatc agccagcgcg cgttcttcac cccctccagc cgcgtgcaga
    20941 tcgcgcttcg caaggtactg atgcgcggac agaagctgcc gctgaccggg cgcctcctgg
    21001 agcgcatgag ggagagcggc aaggcgagcc gcatgaagga aacggacatt gcgcgggcat
    21061 gatgagggct accccggccg cggggatcca gcctgcgcag acgggagatc ggtgatggct
    21121 gcagtgaccc gacacgactc ggggaaggcc acacgcatcc tcaccgtcac cagggagctg
    21181 gtactcaggc gcggggtcag gggcgtgacg atcgctgaga tcgccgagaa ggcacatgtc
    21241 ggcaagggca cggtgcatct gtacttcggc accaaggagg acctggtcgt cgggctgttc
    21301 gctcgggact tcctggccgt ggtggagcgg tacatccaga cgctcaccac cgagcccgac
    21361 caggtccgcc cacaacggct gtgcccactg ctggttcgca ccgccctgga ccacccgttc
    21421 gtgcgcgcac tgcagaccgg cgacgtcgag atgctcggca tcctcggcga gcacccactc
    21481 agcaaggaac tgctcggcac gctcggcccg gggtcgatga tgtactcgtt gctcccggtg
    21541 tggcgccgat accagttggc ccgcacggac tgggcgctgg acgaacaggc ctacgcactg
    21601 cgcgcgatca tcgccggatt cttcgaggtc gcggcccatc cccacgttct gccggacgtc
    21661 acggtcgact ttccggagca ggtcatggcc agggcggtgg ccgcgttgtt gggccctgaa
    21721 caggcgaacc cggcggacgt ccgcgccgcc gctcaggagt gcgtgcggat gctggccgac
    21781 tcacgcgcgg tcatactcgc ctcgatcgcc ccggcatagc aatgaggcga gctgacgcac
    21841 gcaggtgtca gcggaggatc actccagctc cgccgcagcc gcggagaact gcgcggagta
    21901 cagatcgtgg taggcacccc cggccgcgag gagctgctcg tgcgtgccct gctccacgat
    21961 ccgccccgac tgcatcacga ggatcaggtc ggcatctcgg atcgtggaga gccggtgggc
    22021 gatcacgaag ctggtgcggt ccgaccgcag cgccgccatc gcccgctgca ccagcacctc
    22081 ggtgcgggtg tccaccgagc tggtcgcctc gtccaggatg agcagcgtcg ggtcggccag
    22141 gaacgcccgc gctatggtga tcagctgctt ctcccccgcg ctgacgttgg agccctcgtc
    22201 gtcgatcacc gtgtcgtagc cgtcgggcag gctgcggacg aaccggtcga cgtaggtcgc
    22261 ccgcgcagcc gcgaggatct gctcctcgct cgccgtcgga tcgccgtaac cgatgttggc
    22321 gcggatcgtg ccgccgaaca gccatgtgtc ctgcagcacc atgcccacgc ccgagcgcag
    22381 cgtggcgcgg accatcgtgg ccgtgtcgac accgtcgagg gtgatccggc cggagtcgag
    22441 ctcgtagaac cgcatgacca ggttcaccag cgtggtcttg cccgccccgg tcggcccgac
    22501 gatcgcgacg gtctgccccg gctccgtggt gagcgacagg tcatcgatca ggggcgcgtc
    22561 cggctggtac cggaacgaga cgtcctcgaa gaccacccga ccgcggcggg tggacggcac
    22621 cacgggcacc gccggatcgg gcacctgctc ctcggcgtcg agcagctcga acacgcgctc
    22681 ggccgaagcc acgccggact gcagcagatt ggccatcgag gccacctgcg tcaggggctg
    22741 cgtgaactgg cgggagtact ggatgaacgc ctggacgtcg cccagcgaga tcgagccgtt
    22801 ggccaccttg agcccgccga ggac
//
LOCUS       DASRSC010000083        13935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1826891,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000083 DASRSC010000000
VERSION     DASRSC010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 13935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13935
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826891"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VFE39_04195"
     CDS             <1..1167
                     /locus_tag="VFE39_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HET6257833.1"
                     /translation="KVVGEGGNLGLTQLGRIEFARAGGKINTDAIDNSAGVDCSDHEV
                     NIKILLDRLVVAGELEWDARNRLLAAMTDEVAEHVLADNRAQNAVLGISLTDAADTVA
                     VHARIVADLVARRGLRPALDVLPEPAEFAELVSEGQGLSSPELATLLAHVKLDLKADI
                     IPTDVPDLPAVRDRLVGYFPPALTLRHPVAVREHPLQREIVATSLVNEMIDRGGMTCP
                     FLLREETSATTADVVRAYLVTTAIFDLPDLWKQIDALAGTVPTPVADEVVRESHQLVA
                     RATHWLLAHRPRPLPVAAETLRFGPPVSTLQPRLSTLLTGHEAETVSSRAEALTKRGV
                     PHRLALRTAAMLHSVGLLDVVEVAQSMGAEGARLPVDEVARLYYTLSARQRYTV"
     gene            complement(1264..1884)
                     /locus_tag="VFE39_04200"
     CDS             complement(1264..1884)
                     /locus_tag="VFE39_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257834.1"
                     /translation="MYARSTTVLAHPDSIDAGVGHVRDEVMPVVQGMDGCIGLSMLVD
                     RTSGQCIVTTAWQTEEAMHASEERLRPVRERAAEVLGGRPQVDEWEIAVLHRDHTSAP
                     GACVRATWVQLEPHQADRIVDVYKIGLLPEIQEFDGFCSASLMVDRASGYAVSSVTFD
                     SHEAMMRTRNLAAVLRERGAREASGEVVEVGEFELALAHLRVPEMA"
     gene            complement(2028..4361)
                     /locus_tag="VFE39_04205"
     CDS             complement(2028..4361)
                     /locus_tag="VFE39_04205"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET6257835.1"
                     /translation="MAVGRHPRDLVVLFIAAAVVVLCSLAARVPVVNPVEAAIFQQFQ
                     QIPPASTMVWRVLSWVGSWAGIAAVAAVALYLKRIRLGLQCAGAGVFAWGLTWAINDL
                     VGSRPVPAELLDAAGVRLVGPEGFAFPAPHAAVAAAMVAVATPYLLKARYRGVAWAVV
                     VLVAAADVYLGNSLPLSAFAGVFLGWGIGALSHLAWGAPGRRTSEAAVRRALERAGLS
                     PVQTVPIRAHLLGPLEFGVTTEAGDRLRVKVVRRLHRRAGPLYRLRRLLASLEVEDEP
                     RLSTPYQETEHEALVSLFAQRAGLRTPPIVLNCEVRHGAPLLIRRQIDGRKLANLSGE
                     EIDEQLLDAIWIQVGALGEARIAHHDLRTKSVLVDAKGNPWLLNFTFGVIGASAARIA
                     QDLAEALVSLASRVGVRRAVRSACRNLSPDQLEPALVYLQPLALPRPIRKQLSDERYV
                     LPDLRETLAEQIDRPIPTFRSPLRPATVVGLLLLAGAVYTLLPQLSSMRAVIDSLWRA
                     NWVWLAVATVTGLVAIVLSSVSIIGSSATPLPFWRTTAVQLAAAFTGRTTPGGVGFFG
                     INIAFMERLGIRRSSAVGVTVLNMAATSVIGGVWCLIGVLGIGASGLLQHVRIPHGWP
                     VLLAAACVLLAATAVLCSPMGRRKFVRPGLRVTRELVGALRQPVRAVQLFGGATGYLV
                     MSGLGLAATLAAFDVHVPVLAVIGVFMIGQTLGHIAPIPGGLGPTEALMIAGLTALGT
                     APTVAVATVLTMRLLTYWLPVLPGIATFRYLQHHGIV"
     gene            complement(4509..5480)
                     /locus_tag="VFE39_04210"
     CDS             complement(4509..5480)
                     /locus_tag="VFE39_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005448362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HET6257836.1"
                     /translation="MQDTDNVQSRAAWPSLRVADWQPTRDTLQLWTQVIGKIRLANAP
                     LINHWWNVPLYVTSRGLTTSLVPHASGRSFQIDFDFLAHRLEIVTTDGAVRTLALEPR
                     TVADFYARVLTHLDDLGLTTRIWPMPVEIEGAIAFDEDAVHASYDGDQVQRFWRILVQ
                     LDRVLHDFRGRFVGKASPVHLFWGALDLAVSRFSGRTAPPHPGGAPNCGPHVMHEAYS
                     HEVSSCGYWPGGDGEGVVYSYVYPEPEGFRDAAVGPEGAHYSEELGEFVLPYERVRTA
                     ADPDRTLLEFLQSSYEAAADSASWPRSRLERASPSYPFSATPSQIAK"
     gene            5702..6796
                     /locus_tag="VFE39_04215"
     CDS             5702..6796
                     /locus_tag="VFE39_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257837.1"
                     /translation="MHSVVAVLVGLAGAVCVLLTLVSAVKTVVVPRAAPVLITRLVFV
                     VMRALFGVALWRSRDYRSKDRVMALYAPFCLLTLPVVWLVLVVAGYTAAFWSLGVRPW
                     REAFLESGSSMLTLGFRPPTDIGTAMLTFSEAVLGLGLLALLVSYLPSIYASYSRREL
                     MVTALDTQAGSPPSAAELLEREARIDGIGRLDTFWQEWARWFADIAETHTSTPSLVYF
                     RSPQPDRSWVTAAGAVLDAAALVASTLDVPKQPSADLCIRSGYLALRNIGDFFVMPFD
                     PDPAPDDPISITRAEYDAVCLRITEAGGKLKPDRDQSWRDFAGWRVTYDAVLLQFASL
                     CMAAPAPWSSDRAVSFTRAPVTRRRARSRL"
     gene            complement(6845..8605)
                     /locus_tag="VFE39_04220"
     CDS             complement(6845..8605)
                     /locus_tag="VFE39_04220"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6257838.1"
                     /translation="MAQSLERPSDDSIDVGALLAEPLLRNTLVAGLAGIHQPVHWCLP
                     LSEMDEPDRGDDPPHSGIVVHAHAARLRGPGGADAVRAAAGRGAVAVLVQTEPGDVAT
                     PSELPFAFPEARRAADEATLPLALLPPTADYRAVSQLVATKVLAQATHVLQYSDRVHR
                     SLGEILARGAGISPLAYGMARMAEAAVMVIDLEGTVLAYEATSATKKLDAAVIIGLLV
                     QHLEQLAADEPATPGVTVLDPVVDGDATCTPIVAPVMYGGEISGLAAVLEPSGGDPHD
                     RAQRRIVAAEGAVLVGSEMLRIRSMTEAEERTRGDFVVGLVHGRFQDGQQLVTRARHH
                     GFHIDGRYAVCVAAMTPPDVDDERARRRVTAVVRAAEKMGSTGGPATLATHIGGNLVV
                     AVPVTRPGRASPDALGEQELVRAFAGQLRKLIGERMSTQVHVAFGRVGTGARGVATSY
                     REARTALALGRRVDVPPIAGYDELRIFVALSDLADSENGRTFAQEVLGPLRKVDGHAR
                     NLESVVLAYITESGNLNAAARRLGLHRNTTLYKLDRVSRTLGMDIRSADTQFMVWLAH
                     HIDNLVQVNDSLDAELAPPP"
     gene            8717..10144
                     /locus_tag="VFE39_04225"
     CDS             8717..10144
                     /locus_tag="VFE39_04225"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6257839.1"
                     /translation="MHSAPIATEVPGESVDPASPGRVVARYAEATAADTRAAVAAAAA
                     AAAGWAAAPPLRRATVATAIADLIDRRSDDIAALITREEGKPLGAARGEAGKSAEQFR
                     LAAQLAYLVEGTTYPQEASATFAYTLRSPLGVVAAITPWNFPLSLAARKIAPALAAGN
                     AVVFKPSPVTAGVGSWFAEVAAEAGVPDGVLQIVHGQDPEAMTALVSAGDVGGISFTG
                     SDAVGAALRRNANGRARLQLELGGHNAAVVCADADLDAAAAKVAGGAFDLTGQACTST
                     DRVLVAESVAAEFTELLAGKVRALRVGHGDRAGVTTGPVATARQFERLTQLKDSALAA
                     GAHVVAEARLDDERDGDGWWITPTLFAGVPAGHPLVDGEVFGPYLSVVPVGSVEQALR
                     VVNSSAHGLVTAVHTRDLGVAHRFAQQAACGIVKINDRTTGNGIAPPFGGWKASSGGA
                     FPEGGRQALDFVTDTKTVYLNYGER"
     gene            10144..10599
                     /locus_tag="VFE39_04230"
     CDS             10144..10599
                     /locus_tag="VFE39_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HET6257840.1"
                     /translation="MQTIHRLTLEDALVLLRAAEEEATRIGVKQTICIADDGAHPIAL
                     HRMTGARLTGVEIAIAKAFTAAGHQRATHLFNEPPNGPALPGNEAFGIQHMQSGRFAV
                     FVGGFPIVYRDQVIGAVGVSGGNGEQDKAVGAAALAAFEKHVVVALTTT"
     gene            complement(10619..12022)
                     /locus_tag="VFE39_04235"
     CDS             complement(10619..12022)
                     /locus_tag="VFE39_04235"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6257841.1"
                     /translation="MSGALRAPHREPTARGEARLGLVATALVVGTFASTIANTLVNVP
                     LAMIVSDLGAPLRSGALVVIAFNMLCALMLPLGGWLGDRFGRRRLFLVSMAGVTIGAA
                     GAALAPNLPVLVTFRAVQGVAGALVLPSVLALLTSAAGAARRGRAVSWWAASNGAGQA
                     AGPTVGGLLADTLGWRSVFVAIVPFAVFAFVGALLWVPRSRAQPVRLEWRGALTLTLG
                     VGLLLTAASAVPPLGPGSPVVWGSGALGVLVLVAFVLVERRQATPFLPPSLLVEPRFA
                     RSSVAALCQMFCLTATLLTVPLYLASRSGASAAIAGLVVVALPLAMTLLAPLAGLATE
                     RWSPRWALRSGLLALGLAELVLAALLMGGQPIGVPLIAAVTAIGVGIAFTQTPATAGA
                     ARTAGQAVGAGLGLFNMMRFVGAALGGAAVAVVLGDAADGTAGYATVAAISGAAALLA
                     LTVTFLGRNPTEERGTA"
     gene            complement(12022..12507)
                     /locus_tag="VFE39_04240"
     CDS             complement(12022..12507)
                     /locus_tag="VFE39_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET6257842.1"
                     /translation="MPESTPADAPVPDPDDGLPPPPRPQGSYVPAVLVDGIAYSAGMT
                     PRRDGRLVVRGTVGADVSLDEAALAAGLAAGNALSAVAAVAGGLERVRRCVRMTVYVA
                     CAKDFVALSAVADGASAVLAQHVGAAALPARSAVGVRRLPDGAPVEVELTAAIVNGAA
                     D"
     gene            12794..>13935
                     /locus_tag="VFE39_04245"
     CDS             12794..>13935
                     /locus_tag="VFE39_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257843.1"
                     /translation="MGLTAAHWVYLAGVLLIIGVTVARKNVVVPALLATLVTAWVMTG
                     SAVNGIQAIFNASLTAASELFNIFLIIALVTALLGTLRAMKADQRMVTPFQRVMRGGW
                     SAYAVLAVVTYVVSLFFWPTPAVPLIGAILVPAAIRAGLPAMGAAMAIAIAGQGMALS
                     SDYVIQVAPGLSAVAAGVQTSAVADIALVLSLVTGGVALVITGVMMRRHMRAPGGTNL
                     EEWEGTVVAPARPFESTRSRIGEVISEKLGIAKSRPSVEIIAEPLTVVEDRVPVGAGV
                     ATRGGGGGEPPVDVLPPPGGGGDAGDGPGGDSAELAAAAADDEQDARTARLSRIFAIG
                     VPVVFAVMVVYMALGKFTTLVPPLAGGSAAGLVGGVAALLVIVAVVA"
BASE COUNT         2081 a         4925 c         4972 g         1957 t
ORIGIN      
        1 aaggtcgtgg gggagggtgg caatctcggg ttgacccagc ttgggcggat agagttcgcg
       61 agggccggcg gcaagatcaa tacggatgcg atcgacaact cggccggcgt ggactgctcg
      121 gaccacgagg tcaacatcaa gatcttgctg gatcggttgg tcgtggccgg cgagttggaa
      181 tgggatgcgc ggaacaggct gctggcggcg atgaccgatg aggtggccga gcacgtgctg
      241 gccgacaacc gggcgcagaa cgcggtgctc gggatcagcc tcaccgacgc cgcggacacg
      301 gtcgcggtgc acgcgcggat cgtcgccgac ctcgtcgccc ggcgcgggct gcggcccgcg
      361 ctcgacgtgc tgccggaacc ggcggagttc gccgagctgg tgagcgaagg ccaggggctc
      421 agctcgcccg agttggccac gctgctcgcg cacgtcaagc tcgatctcaa ggcggacatc
      481 atcccgacgg atgtgcccga tctgccggcc gtgcgggacc ggctcgtcgg ctacttcccg
      541 cctgctctga ccctccgcca tcccgtcgca gtgcgggagc acccgctgca gcgcgagatc
      601 gtggccacct cgctcgtgaa cgagatgatc gaccgcggcg ggatgacctg tccgttcctc
      661 cttcgggagg agacgtcggc gaccacggcc gacgtcgtgc gcgcctacct cgtcaccacc
      721 gcgatcttcg acctgcccga cctgtggaag cagatcgacg ctctggcggg gaccgtgccc
      781 acgcccgtgg ccgacgaggt cgtccgcgag tcgcaccaac tggtcgcccg cgcaacgcac
      841 tggctactcg cccatcggcc ccggcccctt cccgtggccg ccgagaccct ccgcttcgga
      901 ccaccggtca gcacgttgca gcctcggctc tccaccctgc tgaccgggca cgaggccgag
      961 acggtcagct cgcgggccga ggcgttgacc aagcgggggg tgccacatcg actggcgctc
     1021 cggacggcgg cgatgctgca cagtgtcggc ctgctcgacg tcgtcgaggt cgcacagtcc
     1081 atgggcgcag agggcgcgcg gctccctgtg gatgaggttg cccggctgta ctacacgctt
     1141 tccgcgcgcc agcgatacac cgtttgacgc acccatgatg caccggaaat ggcggcggac
     1201 ggggagcgcc cggccgcgcc cccgcaggcg ctccccgtcg tcgcgatctt gccgcgcgcc
     1261 cggtcaggcc atctcgggaa cccgcaggtg ggcgagcgcc aactcgaact cgccgacctc
     1321 gacgacctca ccgctggctt ccctggcacc gcgctcccgc agcacggccg ccaggttgcg
     1381 ggtgcgcatc atggcctcgt gactgtcgaa cgtcacggac gagacggcat agccggatgc
     1441 gcggtcgacc atgaggctgg cgctgcagaa cccgtcgaac tcctggatct ccggcagcag
     1501 cccgatcttg tagacgtcga cgatccggtc ggcctggtgg ggctccaact ggacccacgt
     1561 ggcgcggacg cacgcgcccg gcgccgaggt gtggtcccgg tgcaggacgg cgatctccca
     1621 ctcgtcgacc tgcggccggc cgccgagtac ctcggcggca cgctcgcgga cgggccgtag
     1681 ccgctcctcg ctggcgtgca tggcctcctc ggtctgccat gcggtggtga cgatgcattg
     1741 acccgacgtg cgatcgacca gcatggacag gccgatgcac ccgtccatcc cctgcaccac
     1801 cggcatgacc tcgtcgcgga cgtgcccgac ccccgcgtcg atcgaatccg gatgtgcgag
     1861 aaccgtggtg gagcgtgcgt acacgatcca ccccctttcg atcagggcag cgcccggcga
     1921 cgccgccggt cgtcgcacac ccttctcccg cggtactggt gtggcaatga cagctaccag
     1981 ttcggacgag cggcctgcgc tggtcagcgc cgccccatgg ccacgggtca gacgatgccg
     2041 tggtgctgca ggtatcggaa ggtggcgatg cccggtagca ccggcagcca gtaggtcagc
     2101 agccgcatgg tgaggaccgt cgccacggcc acggtcggag cggtgcccaa cgcggtcaga
     2161 ccggcgatca tcagcgcctc ggtggggccg aggccgccag ggatgggggc gatgtgcccg
     2221 agggtctggc cgatcatgaa tacgccgatc accgccagca cggggacgtg cacgtcgaac
     2281 gcggccaggg tcgccgccaa cccgaggccg gacatcacca ggtaaccggt ggctccgccg
     2341 aacagctgaa ccgcccggac cggttgtcgc aaggcgccca ccagctcgcg cgtcacgcga
     2401 agcccgggcc ggacgaactt gcggcgcccc atcggggagc agagcacggc ggtggcggcc
     2461 agcagcacgc aggctgccgc cagcagcacc ggccatccgt gcgggatccg gacgtgctgg
     2521 agcaggcccg aggctccgat ccccaagacc ccgatcaggc accagacacc gccgatgacg
     2581 ctcgtggcgg ccatgttcaa caccgtcacg ccgaccgcgc tcgaccgacg gatgccgagg
     2641 cgttccatga aggcgatgtt gattccgaag aagcccacgc cgcccggggt ggtgcgaccg
     2701 gtgaacgcgg cggcgagctg cacggccgtg gtacgccaga acggcaacgg cgtcgcgctc
     2761 gaaccgatga tcgacacact ggagagcacg atcgcgacga gcccggtgac cgtggccacc
     2821 gccagccaga cccagttggc acgccagagc gagtcgatca ccgctcgcat gctggagagc
     2881 tgcggcagca gcgtgtacac ggctccggcg agcaggagca gcccgaccac ggtggcgggc
     2941 cggagcgggg aacggaaggt gggtatcggc cggtcgatct gctccgcgag cgtctcgcgc
     3001 aggtcgggca acacgtagcg ttcgtcgctg agctgcttgc ggatcgggcg cggcagagcg
     3061 agtggctgca ggtagaccag cgcaggctcg agttggtcgg gcgacaggtt ccggcacgca
     3121 ctacgtaccg cacgtcggac gccgacccgc gacgcgagcg aaaccagcgc ctcggcgagg
     3181 tcctgcgcga tgcgagcagc cgaggcaccg atcacgccga acgtgaagtt gagcagccat
     3241 gggttgccct tggcgtcgac gaggacgctc ttcgtgcgca ggtcgtggtg ggcgatcctg
     3301 gcctcgccga gcgctccgac ctggatccag atggcgtcca gcaactgttc gtcgatctcc
     3361 tcgccggaca ggttcgcgag cttgcggccg tcgatctgac ggcggatcag cagcggagcg
     3421 ccgtgccgga cctcgcagtt cagcacgatg ggcggggtgc gcagcccggc gcgttgcgcg
     3481 aacagcgaga cgagcgcctc gtgttcggtc tcctggtacg gggtcgacag ccggggctcg
     3541 tcctccacct ccagcgaggc cagcagccgt cgcagccggt acaacggacc ggcgcggcga
     3601 tgcaggcgtc ggaccacctt cacccgcagt ctgtctccgg cctcggtcgt cacgccgaac
     3661 tcgagtggtc ccagcaggtg tgcccggatg ggaaccgtct gcaccgggga gagcccggcg
     3721 cgttcgagcg cccggcggac cgcagcttcg gacgtccgac ggcccggcgc accccacgcc
     3781 aggtgggaga gtgcgccgat tccccacccg aggaacaccc cggcgaacgc gctcagcggc
     3841 aggctgttgc cgaggtacac gtccgcggcg gcgaccagca ccaccaccgc ccacgccaca
     3901 ccgcggtacc tcgccttgag caggtacggg gtggcgacgg ccaccatcgc cgccgcgacg
     3961 gcggcgtgtg gcgccgggaa ggcgaagccc tcggggccga ccagccggac ccccgcagca
     4021 tcgaggagct ccgcgggcac cggcctgcta ccgacgaggt cattgatcgc ccaggtgaga
     4081 ccccaggcga acactccggc ccctgcgcac tgcagcccga gccggatgcg cttgaggtag
     4141 agcgccacgg cggcaaccgc cgcgatcccg gcccagctgc ctacccagct gagcacccgc
     4201 cacaccatgg tcgacgccgg cgggatctgc tggaactgct ggaagatcgc cgcctcgacg
     4261 gggttgacga ccgggacccg cgcggccaac gagcacagca cgacgacagc ggcggcgatg
     4321 aacagaacga caaggtcgcg cgggtgacgt cccacagcca tcggcggctg atgtcgctcg
     4381 atcgtcgccg tcggcgctcg gtcggtcacg gacccatcgc ccactgatcc cgcctcagcc
     4441 gcagtctcgg tcgatcgctc cagtctgcct gatggcatcg tgaccgggcc tacattccgg
     4501 gcgaacagct acttcgcgat ctgcgacggt gtggcggaga atgggtacga gggactcgcc
     4561 ctttccagcc gtgatcgtgg ccaggaagcg gagtccgcgg ccgcctcgta ggagctctgc
     4621 aggaactcga gaagcgtgcg gtcggggtcg gccgccgtgc ggacgcgctc gtagggcagg
     4681 acgaactcgc cgagctcctc gctgtagtgg gcaccttcgg ggccgaccgc cgcgtcacgg
     4741 aacccttctg gctcggggta gacgtaggag tagacgacgc cttcgccgtc gccacccggc
     4801 cagtagccgc agctgctcac ctcgtgcgag tacgcctcgt gcatgacatg cggcccgcag
     4861 ttcggcgccc ctccggggtg aggaggcgcc gtgcggccgg agaaccggga taccgccagg
     4921 tccagcgcac cccagaacag gtggacggga cttgccttgc cgacgaaccg gccgcggaag
     4981 tcgtgcagca cccggtcgag ctgcaccagg atccgccaga accgctgcac ctggtcgccg
     5041 tcgtaggacg cgtgcacagc gtcctcgtcg aacgcgatgg cgccctcgat ctccaccggc
     5101 atcggccaga tccgtgtggt gaggccgagg tcgtcgaggt gggtcagcac gcgagcgtag
     5161 aagtcggcaa ccgtgcgcgg ttcgagagct aaggtccgca cggctccgtc cgtcgtcacg
     5221 atctccaagc ggtgggccag gaagtcgaaa tcgatctgga agctccggcc gctcgcatgc
     5281 ggaaccaacg aggtcgtcag cccgcgtgag gtgacgtaga gcggaacgtt ccaccagtgg
     5341 ttgatcagcg gcgcgttcgc gagacggatc ttgccgatca cctgggtcca cagctgaagc
     5401 gtgtccctgg tcggctgcca gtcagcaacc cgcagcgacg gccaggcggc ccggctctgg
     5461 acattgtccg tgtcctgcat ctgatctccc agctccgacc tgccacgcaa tctcgtccac
     5521 ccgacaccgt ctccgggtgc cccgcaagtc ggctcagcca accgggccgg ttcatctccc
     5581 cgcgtaacgt cgcgctgggc cctgctcgac gaggagccga agtgggaccg ctcgccggga
     5641 cgacgtgccg cgagcagggc acttcgcctg gtcgcgagcc aacctggagg ggatgtgagc
     5701 catgcacagc gtcgttgcgg tcctggtggg gctggccggc gcggtatgcg tcctgctgac
     5761 cctggtgtcg gctgtgaaga ccgtggtcgt gccccgcgcc gcccccgtgc ttatcacccg
     5821 cttggtgttc gtcgtgatgc gggcgctctt cggcgtggcg ctgtggcgca gccgcgacta
     5881 tcgctccaag gaccgggtga tggcgctgta cgcgccgttc tgcttgttga cgctgccagt
     5941 ggtgtggctc gtgctcgtcg tcgccggcta caccgcggcc ttctggtcgc tcggcgtgcg
     6001 gccgtggcgg gaggcgttcc tggaaagcgg gtcgtcgatg ctcacgctcg gttttcggcc
     6061 gccgaccgac atcggcaccg cgatgctcac gttctccgag gcggtcctcg ggctcggcct
     6121 gctcgcgctg ctggtctcct acctgccgtc catctacgcg tcctactccc gccgcgaact
     6181 catggtcacc gcgctggaca cgcaggccgg cagcccaccg tcggccgcgg agctgctgga
     6241 acgagaagcg cgcatcgacg ggatcggccg gctcgacacc ttctggcagg agtgggcccg
     6301 ctggttcgcg gacatcgcgg agacgcacac gtcgacgccg agcctggtgt atttccgctc
     6361 gccgcaaccc gaccggtcgt gggtgaccgc ggccggcgcg gtactcgatg cggcagcact
     6421 cgtggcgtcc accctggacg tgccgaagca gccgtccgcc gacctgtgca tccgcagtgg
     6481 ctacctggcg ctgcggaaca tcggcgactt cttcgtcatg ccctttgacc ccgacccggc
     6541 accggacgac ccgatctcga tcacccgcgc cgagtacgac gccgtctgcc tgcggatcac
     6601 cgaagccggc gggaagctca aacccgaccg cgaccagagc tggcgcgact tcgccggctg
     6661 gcgcgtcacc tatgacgccg tgctgttgca gttcgcgtca ctgtgcatgg ctgcgcccgc
     6721 gccgtggtcg tccgaccgcg ctgtctcgtt cacccgcgca ccggtcaccc gtcgacgcgc
     6781 ccggtcccgc ctctgactcg cggtcgccgg aagcggaccc cccgcgatcc gtgctgagcc
     6841 ggactcatgg tggcggtgcg agctcggcgt ccaggctgtc gttgacctgg acgaggttgt
     6901 cgatgtggtg cgcgagccag accatgaact gggtgtccgc ggaccggatg tccatgccga
     6961 gggtgcggga gacccggtcg agcttgtaga gggtggtgtt gcggtgcagg ccgagcctgc
     7021 gcgccgccgc attgaggttg ccggactccg tgatgtaggc gaggacgacg ctctccaggt
     7081 tgcgggcgtg gccatcgacc ttgcgcaggg gtccgagcac ctcctgggcg aaggtccggc
     7141 cgttctcgct gtcggcgagg tcgctgaggg cgacgaagat gcggagctcg tcgtagcccg
     7201 cgattggcgg gacgtcgacc ctgcggccga gcgcaagtgc cgtgcgtgcc tcgcgatagc
     7261 tcgtcgcgac cccgcgtgcc ccggtgccga cccggccgaa tgccacgtgt acctgcgtgg
     7321 acatccgctc gccgatgagc ttgcggagct ggccggcgaa ggcgcggacg agctcctgct
     7381 cgcccagggc gtcgggggag gccctgccgg gacgggtgac cggcacggcg acgacgaggt
     7441 tgccgccgat gtgggtggcg agagtcgcgg gcccacccgt cgagcccatc ttctcggccg
     7501 cgcgcaccac ggcagtgaca cgccgacggg cccgctcgtc gtccacatcg gggggagtca
     7561 tcgccgcgac gcagacggcg tatcggccgt cgatgtggaa gccgtggtgg cgggcgcgcg
     7621 tcaccaactg ctgcccgtcc tggaaccggc cgtggacgag gccgacgacg aagtcccctc
     7681 gcgtgcgttc ctcggcctcg gtcatcgagc ggatgcgcag catctccgag ccgaccagta
     7741 ccgctccctc cgcggcgacg atccggcgtt gcgcccggtc gtgcgggtct cctcccgagg
     7801 gctcgagcac agctgcgagc ccgctgatct ccccgccgta catcacgggc gccacgatcg
     7861 gcgtgcaggt ggcgtcaccg tccacgacgg gatcgaggac ggtgacgccg ggcgtcgccg
     7921 gctcatcggc ggcgagctgc tcgaggtgct ggacgagcag gccgatgatc acggccgcgt
     7981 cgagcttctt cgtcgccgac gtcgcctcgt acgccaagac ggtgccttcc aggtcgatca
     8041 ccatgacggc ggcctcagcc attcgagcca tcccgtacgc cagcggcgag atccccgccc
     8101 cgcgggcgag gatctccccg agagaccggt gcacccggtc gctgtactgc agcacgtggg
     8161 tggcctgggc caggaccttg gtggcgacga gttggctgac ggcgcggtag tccgccgtcg
     8221 gtggcagcag ggcgagaggg agcgtggcct cgtcggcggc acgccgcgcc tcgggaaagg
     8281 cgaagggcag ctcactcggg gtggcgacgt cccctggctc ggtctggacg agcacggcga
     8341 cagcgccgcg accggctgcg gcccggacgg cgtccgctcc acccggtcca cgcagtcggg
     8401 cggcgtgggc gtgtacgacg atccccgagt gcggggggtc gtcgccccgg tcgggctcgt
     8461 ccatctcgga cagcggcagg caccagtgca ccggctggtg aatgcccgcc agtccggcca
     8521 ccagggtgtt ccgcaggagc ggctcggcga gcagcgcgcc cacgtcgatg ctgtcgtcgc
     8581 tcggccgctc caggctctgt gccatgtgcc cacatctcga tccgtcgagg gtgcagttcg
     8641 tccagtgcca gacatggtga cccgaccgca gaatcaccgg aagacgtcat tgtccacaat
     8701 cattcggagg ttcttcgtgc actccgcacc aatcgccacc gaggtcccgg gcgaatcggt
     8761 cgatccggcc tcgcccgggc gcgtcgtggc ccggtacgcg gaggcgaccg ccgccgacac
     8821 ccgcgcggca gtggctgccg ctgccgcggc tgcggccgga tgggccgcgg cgccgccgct
     8881 gagacgggcg acggtcgcca ccgcgatcgc cgacctgatc gaccggcgct ccgacgacat
     8941 cgccgcgctg atcacacgcg aggagggcaa gccgctcggc gccgcgcgcg gcgaggcggg
     9001 caagtccgcg gagcagttcc ggcttgccgc ccagctggcc tatctcgtgg aggggacgac
     9061 ctacccgcag gaagcgagcg cgaccttcgc ctacacgctg cgcagtccgc tgggcgttgt
     9121 cgcggcgatc acgccgtgga acttcccgct gtcactggcc gcacgcaaga tcgcgcccgc
     9181 gctcgcggcg ggcaacgccg tcgtgttcaa gccctcgccg gtgacggcgg gggtgggctc
     9241 ctggttcgcc gaagtggcgg cggaggcagg cgtgccggac ggcgtgctgc agatcgtcca
     9301 cgggcaggac ccggaggcga tgacggcgct ggtgtccgcg ggggacgtcg gcgggatcag
     9361 cttcaccggt tccgacgcgg tcggcgcggc actgcggcgc aacgccaacg gccgcgccag
     9421 gctgcagctg gagctcggcg ggcacaacgc cgccgtcgtc tgcgctgacg ccgatctcga
     9481 cgcggctgcg gcgaaggtgg cgggcggagc gttcgacctc accggtcagg cgtgcacgtc
     9541 caccgaccgc gtgctggtag cggagtcggt cgcggcggag ttcaccgagc tgctcgccgg
     9601 gaaggttcgg gcgttgcgcg tggggcacgg cgaccgcgcc ggcgtcacca ccggcccggt
     9661 ggcgaccgcc cgccagttcg agcggctgac ccagttgaag gactcggcgc tcgccgcagg
     9721 cgcacacgtg gtggccgagg cccggctcga cgacgagcgg gacggcgatg gctggtggat
     9781 cacccccacc ctgttcgccg gcgtgcctgc cggacatccg ctggtcgacg gcgaggtctt
     9841 cgggccgtac ctgtcggtgg tgccggtcgg ctcggtcgag caggcgctac gggtggtcaa
     9901 ctcctcggcg cacggcctgg tcaccgccgt ccacacccgg gatctgggcg tggcgcaccg
     9961 gttcgcgcaa caggcggcct gcggcatcgt caagatcaac gaccggacga cgggcaacgg
    10021 gatcgccccg ccgttcggcg gctggaaggc ctccagcgga ggcgcgttcc ccgagggcgg
    10081 ccggcaggcc ctcgacttcg tgaccgacac caagaccgtc tacctgaact acggagagcg
    10141 ctgatgcaga cgatccaccg cctgaccctc gaggacgcac tggtgctgct gcgcgccgcg
    10201 gaggaggagg ccacccggat cggggtgaag cagaccatct gcatcgccga cgacggcgcg
    10261 caccccatcg cgctgcaccg tatgaccggt gcgcggctca ccggcgtcga gatagcgatc
    10321 gcgaaggcct tcaccgcggc gggacaccag cgcgccaccc acctgttcaa cgagccgccc
    10381 aacggacccg cgctgccggg caacgaggcg ttcggcatcc agcacatgca gtccggccgg
    10441 ttcgccgtgt tcgtcggcgg cttcccgatc gtctaccgcg accaggtcat cggcgcggtc
    10501 ggcgtgagcg gcggcaacgg cgaacaggac aaggcggtgg gtgccgcggc gctggccgcg
    10561 ttcgagaagc acgtcgtcgt cgccctcacc accacctgag ggcaggcacg gcccttgctc
    10621 aggccgtgcc acgttcctcg gtggggttcc tgccgaggaa cgtgacggtc agcgccagca
    10681 gcgccgccgc cccgctgatc gccgcgacgg tggcgtaccc ggcagtgccg tcggcagcgt
    10741 cgcccagcac cacggcgacc gcggcaccgc ccagagcagc gccgacgaag cgcatcatgt
    10801 tgaacaaccc cagtccggca ccgaccgcct gccccgcggt acgcgcggca cccgcggtgg
    10861 cgggggtctg ggtgaacgcg atccccacac cgatggcggt gaccgccgcg atcagcggca
    10921 ccccgatcgg ctgcccgccc atgagtagcg ccgcgagcac gagctccgcg aggccgagtg
    10981 cgagcagacc cgagcgcagg gcccaccgtg gactccaccg ttcggtggcc agcccggcca
    11041 gcggcgccag cagcgtcatg gccagcggca gggcgaccac gacgagcccg gcgatcgcgg
    11101 cggacgctcc ggaccgtgac gcgaggtaca ggggaaccgt cagcagcgtg gcagtgaggc
    11161 agaacatctg gcacagcgcc gccaccgacg accgggcgaa ccggggctcg accagtaggg
    11221 acggcgggag gaacggcgtg gcctgccggc gttccaccag cacgaaggcg accagcacga
    11281 gtaccccgag cgccccactg ccccacacga ccggtgagcc cggccccagc ggaggtaccg
    11341 cggacgccgc ggtgagcagc agccccaccc cgagagtgag cgtcagcgcg ccgcgccact
    11401 ccagccggac cggctgggct cggctgcgag ggacccagag cagcgcgccg acgaacgcga
    11461 aaacggcgaa cgggacgatc gcgacgaaca cggagcgcca gcccagcgtg tcggccagca
    11521 gccccccgac ggtggggccc gccgcctgcc ccgccccgtt cgacgccgcc caccaggaca
    11581 cggcgcggcc ccgtcgggcg gccccggctg cgctggtcag cagggcgagc acgctcggga
    11641 gcaccagcgc gcccgccacc ccctgcacgg cgcggaaggt gacgagcacc gggaggttcg
    11701 gggcgagcgc cgcgcctgcc gcgccgatcg tgacccccgc catcgagacc aggaacaacc
    11761 tgcgcctgcc gaaccggtcg cccagccacc ccccgagcgg cagcatcaac gcacacagca
    11821 tgttgaacgc gatcacgacg agggccccgc tgcggagcgg ggcgcccaga tcgctgacga
    11881 tcatggcgag cggcacgttg accagcgtgt tggcgatcgt gctggcgaag gtcccgacga
    11941 cgagcgcggt cgcgacgagg ccgaggcgtg cctcgccgcg ggccgtcggt tcgcggtgcg
    12001 gtgcccgcag agctcccgac atcagtccgc agcgccgttg acgatggccg cggtgagctc
    12061 gacctcgacc ggcgccccgt cgggcagtcg gcgcacgccg acagccgatc gggccggaag
    12121 cgccgccgcg ccgacgtgct gggccagcac ggcggaggcg ccgtcggcga cggcggagag
    12181 cgcgacgaag tccttcgcgc aggcgacgta cacggtcatc cgcacgcatc gccgcacccg
    12241 ctcgaggccg cctgcgacag ccgccacggc cgacagcgcg ttgcccgcgg ccagcccggc
    12301 agccagtgcg gcctcgtcga gcgacacgtc ggctccgacg gttccgcgca cgacgagcct
    12361 gccgtcgcga cgcggggtca tcccggcgct gtaggcgatg ccgtcgacca gcacggcagg
    12421 gacgtagctg ccctgtgggc ggggcggtgg cggcagcccg tcgtccggat ccggcacggg
    12481 cgcgtccgcc ggggtcgact caggcaccga gtgccacccc ttcctggccc ggggggcgca
    12541 gcgtggccgg gaacggctgc tgaagatcga cgaggtcggt cacacctgca cgatgctgct
    12601 ggtcgtcgtg gggcgacagg gcaacagtgc ccaacttgag tgctgtggct agtgcagtct
    12661 gcctttacga caacgtgcac aatccgaaat gcaggtgatg tgcactgtcc ttctagctgc
    12721 cattcgcgtg gtactggact gtgtcgcccg acacgtcgga tgccgcgctc gtcgcggctg
    12781 gaaggaggcc gcggtgggac tcactgccgc acactgggtc tatctggcgg gtgtcctcct
    12841 gatcatcggg gtcaccgtag cccgtaagaa cgtggtcgtc ccagcactgc tagccacgtt
    12901 ggtcacggcg tgggtgatga ccggaagcgc ggtcaacgga atacaggcga tcttcaacgc
    12961 cagcctgacc gcggcgtcgg agctgttcaa catcttcctg atcatcgcgc tcgtgacagc
    13021 gttgctgggc accttgcgcg cgatgaaggc cgaccagcgg atggtcacgc cgttccagcg
    13081 cgtgatgcgg ggcggttggt cggcgtacgc ggtactcgcg gtcgtgacgt acgtggtcag
    13141 cctgttcttc tggcccaccc cggcggtgcc gctgatcggc gcgatcctcg tccccgcggc
    13201 catccgggcg ggccttcccg ccatgggtgc ggccatggcg atcgccatcg cggggcaggg
    13261 catggcgctg tccagcgact acgtcattca ggtcgcgccc ggtctgtcgg ccgtcgcggc
    13321 cggcgtgcag acctccgccg tcgccgacat cgcactggtg ctctccctcg tcaccggtgg
    13381 cgtcgccctc gtgatcacgg gtgtgatgat gcgccgccac atgcgcgcac caggtggaac
    13441 gaacctggag gagtgggagg gcaccgtcgt cgcgcctgcg cggccgttcg agagcaccag
    13501 gagccgtatc ggtgaggtca tctcggagaa gttgggaatc gcgaagtcac gcccgagcgt
    13561 cgagataatc gccgagccgc tcacggtcgt cgaggaccgg gttcccgtcg gtgcgggcgt
    13621 agcgacacgt gggggaggcg ggggcgagcc tcctgtcgat gtgctcccgc cgccaggcgg
    13681 cggtggcgat gccggcgacg gacccggtgg cgattccgcc gagctcgcgg cggcagccgc
    13741 agatgacgag caggacgccc gcacggcccg cctgtcgagg atcttcgcga tcggcgtgcc
    13801 ggtcgtcttc gccgtcatgg tcgtctacat ggcgctgggc aagttcacca cgctggtccc
    13861 gccgctcgcg ggagggagcg cggccgggct ggtcggtggc gtcgccgccc tgctggtgat
    13921 cgtggccgtg gtcgc
//
LOCUS       DASRSC010000084        22872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2318745,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000084 DASRSC010000000
VERSION     DASRSC010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 22872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22872
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2318745"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..753)
                     /locus_tag="VFE39_04250"
     CDS             complement(<1..753)
                     /locus_tag="VFE39_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET6257844.1"
                     /translation="MSPSPVPSGTVPGPRNAITDVPGVRVGHATRVGGGFLTGTTVVL
                     LPPSGAPAAVDVAGGGPATRDTAALDLRSGGANAHAVVLTGGSAYGLAAAGGVLEWLA
                     ADGRGAQPVGEAPEILLPLVPAAALFDLGRGGVFAARPDAVVGREAVRAAANSRAFAP
                     VAQGCVGAGTGAVTAGMKGGIGTASTVLAGGTVVGALVAVNAHGSTLDPASGLPRAFD
                     LGAEGEFPLTTPSGVEHAAAQHLLAESGRAPTG"
     gene            complement(840..1706)
                     /locus_tag="VFE39_04255"
     CDS             complement(840..1706)
                     /locus_tag="VFE39_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6257845.1"
                     /translation="MIRDWFAGRRGRIHYRRWEPAGRPVALLVLLHGLGEHSGQYAPL
                     AEAAAEAGVETWAPDHAGHGHSEGERVLVESIDDLVADAETLLARARAARPRVPVVVA
                     GHSLGATVAVLLVGEIRTAATDTGADPTADPAAGTAGLVLAGSALLDGGQGLAALVAS
                     GVDPMELRKDPSELVRDPRQAKLVRDDPLVWNGGLRPQTLAALTAAAARTRAVVVDGA
                     LDHVPVLLLHGAEDDLAPVAGALAVVDLLPRARGLVYPLDRHNILHELDRDEVHAELL
                     RFVGMVTSVPIP"
     gene            complement(1703..1891)
                     /locus_tag="VFE39_04260"
     CDS             complement(1703..1891)
                     /locus_tag="VFE39_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257846.1"
                     /translation="MTMIRISTRTLPIIVLSRFRDLAADREEFQADLRRAALRLSAGI
                     DGIDDLLADLATTLERAA"
     gene            complement(2126..2698)
                     /locus_tag="VFE39_04265"
     CDS             complement(2126..2698)
                     /locus_tag="VFE39_04265"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HET6257847.1"
                     /translation="MDVAAGVHVCAVSTGRAERDAVLVTFLGDGLSAGHKCLFGLNDR
                     DPWQLVRGIGLPGEVDRGRASHQLEKLGGFDMRFTTEANSIDGMLSVWAEAVTDSVAE
                     GYDLARLGAEAAWWGPQLDGDAAVLAEYESALNSFVADNPVAILCMYDMHDCDGAQLI
                     ELMRTHPRLMIDGLTFDNPYQLEAANIRIP"
     gene            complement(2963..3322)
                     /locus_tag="VFE39_04270"
     CDS             complement(2963..3322)
                     /locus_tag="VFE39_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257848.1"
                     /translation="MIDEVAVERVRRFCPEGQWEVDYDVVHYADPDGGMWEYYSVNRA
                     PTMLPYTQDSTRLCGLRAQHGGHAPGPTRGALPRQEGMPGRLGGGSRWVNRPERADAP
                     LLDPRAAPAADRTESGA"
     gene            complement(3319..4098)
                     /locus_tag="VFE39_04275"
     CDS             complement(3319..4098)
                     /locus_tag="VFE39_04275"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HET6257849.1"
                     /translation="MAEWDTERFTSLVAGLAGTDRDGAERATAAVLNTLAQHVTRGEA
                     ADVLERLPAELQPHLHTPGPAERFGVAEFVRRVAAREGVDPATAERHAAAVLTVLRQA
                     IGEDEFADLTAQLPREYGPLLSGRSVVDPAEAFVARVADRAGTTVEEAGRLTAAVLET
                     LAQRIAPGEAADLAARLPLALQEPLRRGAAAPQHRMGVADFVIRVAERAGLDLTDAVR
                     GAPAVFAVLRDDVGDEFVDVTVQLPEEYRTLVDRRRSRAPR"
     gene            complement(4112..4483)
                     /locus_tag="VFE39_04280"
     CDS             complement(4112..4483)
                     /locus_tag="VFE39_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain containing protein"
                     /protein_id="HET6257850.1"
                     /translation="MTAEAGERDLGRPVAYLVLADGTPVYEPDGERIGVVDHVLADEH
                     HDIFHGIVVHTVPLPGRHLFADADQIAGLYERGVVLAVGRDALHEPREPERTRVRPDG
                     SAVENPLEARLRRVWDWLTNR"
     gene            4749..4985
                     /locus_tag="VFE39_04285"
     CDS             4749..4985
                     /locus_tag="VFE39_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257851.1"
                     /translation="MTTWTAPARPDTDSDSDDVTAAVERERLLLVRCVETAYESLRLM
                     PGMDTSGRTLVWFAGHLLQAHRRATGTAESAARQ"
     gene            complement(5100..5720)
                     /locus_tag="VFE39_04290"
     CDS             complement(5100..5720)
                     /locus_tag="VFE39_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6257852.1"
                     /translation="MARDNHEVLEVSHDPGLVRLQQAFRWLVLALHLDDGGDNDGARI
                     VTCRRCGKRNRVPAVATGVPRCGNCHDALPWIAGAGDDEFAEVAEQVSIPVLVDLWAT
                     WCGLSWMVRPVVERLAAERAGEIKVVEVEVVRAPRMSQRFQVCGLPTLMVLRGGEVVA
                     RQYGAMQTPALRSWLDDALRDQGGAHPSSTSTATIASRPGLLPLQR"
     gene            5959..6843
                     /locus_tag="VFE39_04295"
     CDS             5959..6843
                     /locus_tag="VFE39_04295"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET6257853.1"
                     /translation="MILVTEATGPVGQRLVEELSDAHAATTAMVPVQAAAQDLPPAVG
                     YVEATLDDPPSAEVLREFDRVFLTSPAREEQVELEVYFVDALVAAGHRPHVVKLAADG
                     FQDPDCRVRFMRNHRQVAVHLEGTGLPVTYLAPGVYTEYLLAATDAIREQGLIPAPAG
                     DGRVAFVAASDVAAVGASALTSGDPGDIHVITGPEALGYADVAARVSAVFARQVDYAD
                     IPVDQARWDMREDGIPEWHAEGMIELFGWISAGACDEVTDTVRTVVGEDPRPLEHWLN
                     EHREAFMFHASGASTQRF"
     gene            6991..7548
                     /locus_tag="VFE39_04300"
     CDS             6991..7548
                     /locus_tag="VFE39_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET6257854.1"
                     /translation="MGSELQGKTIAILATDGVERVELEQPRGALYGAGARSDLLSIHP
                     GEIQARQFDMVSAGTFPVDRQVSDANVDDYDALLLPGGTVNPDQLRMNPGAVDFVKAF
                     VATGKPVAAICHGPWTLVEADVVRGRRLTSWPSVRTDLRNAGAEVVDEEVVIDGQFTT
                     SRSPADLPAFCRAIVEQFAGAPVSR"
     gene            complement(7586..8368)
                     /locus_tag="VFE39_04305"
     CDS             complement(7586..8368)
                     /locus_tag="VFE39_04305"
                     /inference="COORDINATES: protein motif:HMM:NF037526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257855.1"
                     /translation="MAVLDEYHRCRVVVVDRRRARFWDLYQDEMVERGKLRDPDLRKS
                     NYAYGMREHATWNKDELLAKRHYRAVAQRLVEDAGRREFDIIAVGGQEHEIPELLDQL
                     PRNMRDRVVGTFPMHSREEDLGTIRGRAEEVVDRYERAEEARLVAETLETHAAGGLAA
                     VGLADCLWGGATAAVGLLLVQDEELRPGVVCDVDGWMAIEGERCPICGNPLRTTPDVI
                     DELVQSVIDEGGSIEHVVAETPLREQLTAATLRFPLPPLPSD"
     gene            complement(8381..8725)
                     /locus_tag="VFE39_04310"
     CDS             complement(8381..8725)
                     /locus_tag="VFE39_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257856.1"
                     /translation="MLEVETIDRILRMDGDGLPVLSLYARIDPDDRRAFPSRVDELLH
                     EVRLLTKDPDLDRNARLSLRGDVGRIVEAVTEEHLHPRTVAFFSCSGRGLFENVELPR
                     AVRDRHVVDATP"
     gene            complement(8737..9813)
                     /locus_tag="VFE39_04315"
     CDS             complement(8737..9813)
                     /locus_tag="VFE39_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6257857.1"
                     /translation="MRSVRRSDAGANRTSALVLALLLAATAALAGCSAAGQPVPPPPP
                     PFAAELSDEFAAVVQDVLPSVVEISQPGSVGSGIVFDRDGHIVTNAHVVGTGKAFEVR
                     FANSSTVRQATLVYSHPPEDLAVIKVQDGGSLVPARFGAARDMRVGDLVMAMGSPLGL
                     ESSVTHGVVSALGRTIPEPAEPEIGFPGTVLRQTIQTSAPINPGNSGGALVNINHEVV
                     GIPTLAAVNPSIGGAAPGVGFAIPGEIANDIARQIVDNGRVVNSGRAAVGITAVTVTD
                     DTGQPIGAGVAALDPNGPAAAAGLRVGDIITRIEGDEIRTAQDLQTRLAERRPGEAVR
                     LTVKRPPAAEPLTITVTLGELPVT"
     gene            10172..11203
                     /locus_tag="VFE39_04320"
     CDS             10172..11203
                     /locus_tag="VFE39_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 family protein"
                     /protein_id="HET6257858.1"
                     /translation="MADEREEAKVYRDLATRRTGEERQILLSLAEAEERHAAHWARLL
                     GDDAGRVRRGAARMRLLAFLARRFGSVFVLALAQRAETRSPYRSDADATAAMAADERV
                     HEEVVRGLAARGRARVSGTFRAAVFGANDGLVSNLALVLGVIGGGASPSTVLLTGLAG
                     LLSGALSMGAGEYVSVRSQRELLAASAPDVRDAHTVLRELDVDANELALVYRARGVAP
                     EEAHRRAAAVLRSDDPTFSPLANGPGEDDGYDVVGTGVGAALSSFTFFASGAAVPVLP
                     FLFGLNGAPALIAAGFLVGLALLLTGATVGVLSGGPPLRRGLRQLAIGAGAAATTYGL
                     GLLVGAAVG"
     gene            11347..12000
                     /locus_tag="VFE39_04325"
     CDS             11347..12000
                     /locus_tag="VFE39_04325"
                     /inference="COORDINATES: protein motif:HMM:NF019656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HET6257859.1"
                     /translation="MDPPLPEIRIGDRERREVDARLQEALADGMLSLIEYDERAAQCW
                     AARTRTDLDPLTSDLPQAQPPEPPVAAETTPPTLWYAPAAGVAGRAVGGLVTAALVGA
                     GIFVGVQVLSADDGFSVFGSRTVQLSGDQQRAEVGMMFGSVRVVVPDDVRVRLSGTVV
                     FGSMNCDEACRTGSNLREVAVEADGAFGSINIMRQSEVDREASTIRDRNRDEDSEDD"
     gene            12434..14137
                     /locus_tag="VFE39_04330"
     CDS             12434..14137
                     /locus_tag="VFE39_04330"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HET6257860.1"
                     /translation="MTQKPFDFVIVGGGTAGCVLANRLTADPGTRVLVLEAGRPDYWW
                     DLPIQLPIAMGMPVGSSEHDWLYESEPEPHLQSRRLHHPRGKGIGGSSTINGMIYQRG
                     HRADFDRWGAECGTPEWDYAHCLPYFRRLENCLGDEEDAFRGRGGPHTLARGPADGPI
                     FEAFFAAAQEAGHAVLPDINGVEQEGFAPLDQGVRGGRRESAARAYLHPARRRRNLEV
                     RSGTAVSRIHVERGRAVAVRFRQDGGADEVVQAREIILCSGAIGSPQLLQVSGIGNAG
                     ELETLGVPVVHDLPGVGESLQDHLAVHLQHICREPVSMAPVRRKANWPRIVAEALLFG
                     SGPGSRNPMQAGGFVRSSPEQSHPDLMFLLAPLAMHSAERSIPVNEHGYQVHVGVMRS
                     DARGSVKITSPVASRHPAIQLNFLSAPDDRQRWIDAVRIGRELLAQPAMDRLNGGESL
                     PGPGVRSDDEVIEWVTRTARAGLHLACSTRMGMDERAVVDPVTLRVRGLDGLRVVDAA
                     IMPSLTNANTYGPVLMLAEKAADVILGNTPLAPEYPAAPARVAAQPPVAHPAGAASVD
                     R"
     gene            14229..15548
                     /locus_tag="VFE39_04335"
     CDS             14229..15548
                     /locus_tag="VFE39_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257861.1"
                     /translation="MRSALASWRSPQHRDGLALVLSSALSSGIGLLYWVVAARLFAPD
                     VVGVNSTLVSTLTLLGSAAQLNLGSALLRFVPVAGRSARSLVLSCYAVGAGTACATGA
                     VFALGAAWWAPDLLESVGHGQLIAFFALSTPVWAVFVMQDYVLTAIKRATLVPVENLV
                     FALLKIALLMAGGVVAFYGTIAVSWAAATAVLVLVVTVWLVRILPAYGIATAASAVSF
                     RAQTIVGFVSADWAGTLFSLAVNFGLPLLVLARLDADSAATFGVAWAVAFGLYLVSNG
                     MGQSLVAHVSADPDALDAACRSMVTKAMTLLVPAVVVIVPGAGLILSVFGEHYAATGT
                     GLLALAALSALPNVVLLATVNAARVLQRKSIQFGVPAALAFIVIPLAWVLMPFLGLTG
                     VGVALLVGQTMVAVVILLVRWTGPRFDTRGLPPTVAVRSSTAASSGS"
     gene            15915..16853
                     /locus_tag="VFE39_04340"
     CDS             15915..16853
                     /locus_tag="VFE39_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6257862.1"
                     /translation="MSTPPPTTARHVRTPTSRLRFGARERHDYLVFLALAAPNILLIV
                     AFSYRPLLSNIYYSTLNWTLGAATAVPVGFDNYVRFFTSPDTAEIARVTAVFTVATVG
                     GSMLLGLLIALVLNRKVRGAGFARAAVFSPFVLSGVGVGLIWVFMFDPTIGVIAAVLR
                     TMDLPSPQWFNDPSLSLVMVIVVYVWKNMGYAAVICLAGLQAVPRDLLEAAAIDGAGR
                     LRTFRSIILPMLSPTTFFLLITSVLNSLQAFDIIRIMTPLGRGTTTLIYDAYLQAFGG
                     YNRAGYSATVSTVLFGLLVLFTAIQMLVLERRVHYR"
     gene            16850..17734
                     /locus_tag="VFE39_04345"
     CDS             16850..17734
                     /locus_tag="VFE39_04345"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6257863.1"
                     /translation="MTMSATGPEQDTRTHPLAPRNLVAAAVGSFIPLVLAVAVVALPL
                     VWMLLSSFKTPGEVLTRDLSVLPHSLAPDNYLQAAREVPFVRLFVNSAIVTVIGAGIK
                     VLLAIFTAYALVFVRFPAKKLIFAAILVTLMVPPQVSLLPNYMLVSGLGGADTYWGII
                     LPGLGTGFGTFLLRQHFMTLPISILEAAEIDGAGHWRRLTRIVIPISGPSIVTVALVS
                     IVFEWNDYLWPLVIVTDPSMMTLPVGLTLLANTESDALAYGVMMAGSVAVIVPVLIVF
                     ASLQRYVIAGLTQGAVKD"
     gene            17801..19192
                     /locus_tag="VFE39_04350"
     CDS             17801..19192
                     /locus_tag="VFE39_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6257864.1"
                     /translation="MTARFPDTSPHARLVAPNLHRRGFLRLASAAGLTAALAACGGPS
                     TSGSGASPASSAPADIDYTGVQPAAQVTMWSSNPGSSQAVTQQIIDAFHASQPDIKVN
                     LVTAGKDYEEIAQKFQTAQTGGTLPDLIVLSDVWWFRYYMLGSIVPLDSAIKAVGIET
                     GDYQDQLLADYQYAGGQWALPWARSTPLFYYNKAHWAAAGLPDRAPTTWAEFNEWAPK
                     LQAAGTGVQHAFQLPALAGYAGWTFQNNLWGWGGAWSKDWDVTSDSAESVAAMQFLQD
                     AVYKGRWAGVSSNDSANDLSAGAVSATMSSTGSLVGILKTATFDVGAGPLPGGPAATS
                     PVCPTGGAGLGIPKAIPKENQLAAATFLKFLTSPENTVTFAGATGYMPIRKSADISAL
                     VAKAPQSKVAIDQLTVTRTQDRARVFFPGADQEMAKSCANILTQQADVHSEMTALKAT
                     LTGIYDQDVKPNL"
     gene            19243..20487
                     /locus_tag="VFE39_04355"
     CDS             19243..20487
                     /locus_tag="VFE39_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET6257865.1"
                     /translation="MEPAPSIDAATSPFDRRVWRVVGDPLVRGARTAVTALPLAGIGV
                     AVKDLIAVAGQPVGAGNPAWLAEQQPQHRSAPALEALLAAGADVVGIARTDEFAYSLA
                     GTNVHYGTPPNPAAPRAISGGSTSGPASAVALGQAAVGLGTDTGGSIRIPASYQGLVG
                     IRTTHGAIDTAGVLPLAPAFDTVGWLTRDTPTALRVAGVLLDGTQDRALDAARTVVLP
                     AAQRCASPDVSLACDVLRDRLAAGGVLPPVQDDDVPADVLQRWLAAFRTVQAYQAWTA
                     HGAWVRTHPGALGDDIAERFAVAAQVTEAEAERAAGVAEQARAQLQRLLANAVLVLPS
                     SAGGALPLHAAATEVEAERAGTLQLTCVAGLAGAPAVSVPALQTSDGRPAGFCLVGAP
                     GTDRDLLRLAHRIALWSGTFSP"
     gene            complement(20477..21073)
                     /locus_tag="VFE39_04360"
     CDS             complement(20477..21073)
                     /locus_tag="VFE39_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HET6257866.1"
                     /translation="MTTWEAKSAVRAVWWLVLLRGILAILFGLFALFAPGTALLALVF
                     VFGAYAILDGITAIVAGVRHRKEEPHWGWHVVQGVVSLIAGIVAFAWPGVTVLAILFV
                     IAFWSIINGVAQIIESFAMRRRGASTWGWSLASGVVSVLFGIVLLAQPGAGLITLLLL
                     VGAFSLVFGVVLVVWALRLRGAVDALADAGPDRRTSTD"
     gene            complement(21245..22477)
                     /locus_tag="VFE39_04365"
     CDS             complement(21245..22477)
                     /locus_tag="VFE39_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET6257867.1"
                     /translation="MGELVAVVVLALVLLAAIQRPFGGNEAVVGVPAAALVVGVGLVP
                     MDAALATFRQLLPTVLFLAAILAFGHLCAGAGVFAHLATLAGRAAAGRPTRLLALTVA
                     LAAVVTAVLTLDATVVLLTPVVLCTARRVDVEARPDLYACLHLANAGSLLLPVSNLTN
                     LLAFTASGLSFGRFAALMTLPWLLTCLLEWSALRAFFRRDLHGGGTEVDTHDPAPRYA
                     MTVLGVTVVGLVVTSALGVTPALAAFGGVLLLGVPRLLRREVTPREIALQANPGFCVF
                     VVALGVVVDAVTRHGLGSTLVGILPDGDQLLILLVLAFVAAGLANIVNNLPATLVLLP
                     VVAGHPTAVLAVLLGVNIGPNLTYVGSLATLLWRRLLPAAHRPRSGQFHALGAVTVPP
                     VLAAATVALWASSIVLGV"
     gene            complement(22531..>22872)
                     /locus_tag="VFE39_04370"
     CDS             complement(22531..>22872)
                     /locus_tag="VFE39_04370"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET6257868.1"
                     /translation="VVQGLGFVLTESFQLDDEGRMRNPNLRNYRIPTYADAPRTDVIC
                     VPSTDSVGPMGSKGIAECCINPVAPALANAVADATGVRFRHLPLTPDRIYRRLVSEAV
                     KPRPAEVPDTA"
BASE COUNT         3302 a         8370 c         7915 g         3285 t
ORIGIN      
        1 cccggtcggc gcacggcccg actccgcgag gaggtgctgc gccgccgcgt gttccacgcc
       61 cgacggtgtg gtgagcggga actcgccctc tgctccgagg tcgaaggcac gcggcagccc
      121 ggacgccggg tcgagggtcg agccgtgcgc gttgaccgcg acgagtgcgc cgacgaccgt
      181 gccgcccgcc agcactgtgc tcgccgtgcc gatcccgcct ttcatgcccg cggtgaccgc
      241 accggtgccg gctccgacgc agccctgggc aactggggcg aacgcgcgcg agttggctgc
      301 tgcccgcacg gcctcgcggc cgaccacggc gtccggtcgg gcagcgaaca caccgcccct
      361 gccgaggtcg aacagcgccg cggcggggac cagcgggagg aggatctccg gcgcctcgcc
      421 caccggctgt gcgccccgcc cgtcggcggc gagccactcg aggaccccgc ccgccgcggc
      481 gaggccgtag gcgcttccgc cggtgagcac cacggcgtgc gcattcgccc ctcccgaccg
      541 caggtccagc gcggcggtgt cgcgtgtcgc cggcccgcca ccggccacgt cgacggccgc
      601 cggcgccccg gacggcggga gcagcacgac cgtggtcccg gtgaggaagc cgccacccac
      661 ccgggtggcg tgcccgacgc ggacgcccgg aacgtccgtg atcgcgttcc ggggaccggg
      721 aacggtaccg ctgggcacgg ggctgggact catacctgct ccgggtcggg cactgcgacc
      781 agcaccgcca ccgcgagcag caggggaacg agtcgtccga agcgcatcga acctctcctt
      841 caggggatgg ggacggacgt gaccatgccg acgaagcgca gcagctcggc gtggacctcg
      901 tcgcggtcca gttcgtgcag gatgttgtgc cggtcgagcg ggtataccag gccgcgcgca
      961 cggggcagca ggtcgaccac cgcgagcgcg cccgctacgg gggcgaggtc gtcctccgcg
     1021 ccgtgcagca acagcaccgg gacgtggtcg agcgccccgt cgacgaccac ggcgcgggtg
     1081 cgggcagcgg ccgcggtgag cgcggcgagg gtctgcggcc ggagcccgcc gttccacacc
     1141 agcgggtcgt cccggaccag cttcgcctgc cgcgggtcgc gcaccagctc tgacgggtcc
     1201 ttgcgtagct ccatggggtc gacaccggac gccacgagcg cggcgagccc ctgcccgccg
     1261 tcgagcagtg cggaaccggc aaggacgagg ccggccgtcc cggcggccgg atcggcggtg
     1321 ggatcggctc ccgtatcggt cgcggcggtg cggatctcgc cgacgagcag cacggcgacc
     1381 gtcgcgccga gcgaatgtcc ggccaccacc accgggacgc ggggccgtgc ggctctggcg
     1441 cgggcgagca gcgtctcggc gtcggccacc aggtcgtcga tgctctcgac cagcacgcgc
     1501 tcgccctcgc tgtgcccgtg cccggcgtgg tcgggcgccc acgtctcgac gcccgcctcg
     1561 gccgcggcct cagccagcgg cgcgtactgg ccgctgtgct caccgaggcc gtgcagcagc
     1621 accagcagtg ccaccggccg ccccgccggc tcccagcgcc ggtagtggat ccggccccgc
     1681 cgccccgcga accaatccct gatcatgccg ctctctccag tgtcgtagcg agatcggcga
     1741 gcaggtcgtc gatcccgtcg attcccgccg acagccgaag cgcggcccgg cgcagatcgg
     1801 cctggaactc ctcgcggtcg gcagcgaggt cgcggaaccg ggagagaacg atgatcggca
     1861 gggttctggt cgatattcgg atcattgtca tgtacaacct cggcaggagt ggtcacggac
     1921 gatgcggggc tccgcgcggc cgcaccacgc gacaggcggc gaggatcagc gacaggactc
     1981 gtcgaactga tcgccgcgac gcccgaccca gaacgagtcg aggaacgcgc tacgcatccg
     2041 ggccgccgag gtcatgtggg gatgaaacca cgacgggagg cccgcgacca cggccgtggc
     2101 ccgcggtgtg attgacgccg ctccgtcagg ggatgcggat gttcgctgct tcgagctggt
     2161 aggggttgtc gaacgtcagg ccatcgatca tcaggcgcgg atgggtacgc atcagctcga
     2221 tcagctgggc gccgtcacag tcgtgcatgt cgtacatgca caggatcgcc acggggttgt
     2281 cggcaacgaa gctgttgagc gcggactcgt actcggcgag gaccgccgcg tctccgtcca
     2341 gttgcggtcc ccaccacgcc gcctcggcac cgagccgggc aaggtcgtac ccctccgcca
     2401 ccgagtccgt gaccgcttcg gcccagacgg agagcatgcc gtcgatggag ttcgcctccg
     2461 tggtgaaccg catgtcgaac cccccgagct tctccagctg gtgggacgcc ctgccgcggt
     2521 cgacctcgcc cggcagcccg atcccccgca cgagctgcca tggatcccgg tcgttcagcc
     2581 cgaacagaca cttgtgcccc gcgctgagcc cgtcgccgag gaacgtgacg agcacggcgt
     2641 cacgctcggc tctcccggtc gagacggcgc agacatgcac accggcggca acgtccatct
     2701 gcctgcgctg ctcctcgcca tgaccaccca ttgcgggccc cctgtcctct acgacccgcg
     2761 gggccaccag gcccggccgt ccgactgact gccccaagcc cccgaaagaa tgaccgtcct
     2821 cggctcctgc gtcgagtgca agaagacccg gcggtcttgg gccggtcgct ggtccgccac
     2881 tagcccgacc ggctccttcc gctgaaccgc tcccggctac accgtcgacg gccatcgcgt
     2941 ccccggcctc gtcggcccgt ggtcacgctc ccgactcggt tcgatccgct gccggcgccg
     3001 cccgcgggtc gagcagcggc gcgtcagcgc gctcgggccg gttcacccag cgactgcccc
     3061 cgccgagtcg tccgggcatg ccctcctgtc tgggcagtgc accacgcgtc gggccaggag
     3121 catggccacc gtgctgcgcc cgcagaccgc acagccgagt gctgtcctgg gtgtagggca
     3181 acatggtcgg ggcgcggttc accgagtagt actcccacat cccgccgtcc gggtcggcgt
     3241 agtggaccac gtcgtagtcg acttcccact ggccctccgg acagaagcgc cggacccgct
     3301 cgaccgcgac ctcatcgatc atcgcggagc tctggaacga cggcggtcga ccagcgtccg
     3361 gtactcctcg ggcagttgca ccgtcacgtc gacgaactcg tcgcccacgt cgtcgcgcag
     3421 cacggcgaac acggcagggg ccccccggac cgcatccgtc aggtcgagcc cggcacgctc
     3481 ggccacccgg atcacgaagt ccgccacccc catccggtgc tgcggcgcag ccgcgccgcg
     3541 gcgcaggggt tcctgcaggg ccagcggcag gcgggcagcg aggtcggccg cttcaccagg
     3601 cgcgatccgc tgggcgagcg tctccagcac cgcagccgtg agacggcctg cctcctcgac
     3661 ggtcgtgccg gcgcggtcgg cgacccgggc cacgaacgcc tcggcggggt ccaccacgga
     3721 gcggccagac aggagcgggc cgtactcgcg gggcagctgc gcggtgaggt cggcgaactc
     3781 gtcctcaccg atggcctggc ggagcaccgt caacaccgcg gccgcgtgcc gttccgccgt
     3841 cgcggggtcc accccctccc gggcggccac ccggcgcacg aactccgcca cgccgaaccg
     3901 ctcggccggc cccggcgtgt gcaggtgggg ctgcagctcc gccgggagcc gttccagcac
     3961 gtcggcagcc tccccgcggg tgacgtgctg ggccagggtg ttcagcaccg ctgccgtggc
     4021 gcgttcggcg ccgtcgcggt cagtgccggc cagccccgcg acgagcgacg tgaaccgttc
     4081 ggtgtcccac tcggccatgt gcctctcccg gtcagcggtt cgtgagccag tcccacacac
     4141 ggcgcagccg agcttcgagc gggttctcca cagcggaccc gtcggggcgc acccgggtgc
     4201 gctccggctc cctcggttcg tgcagcgcat cccgcccgac cgcgagcacg accccgcgct
     4261 cgtagagtcc ggcgatctgg tcggcgtcgg cgaagaggtg ccggccgggc agcggaacgg
     4321 tgtgcaccac gatcccgtgg aagatgtcgt ggtgctcgtc ggcgagcacg tgatccacga
     4381 ccccgatccg ttccccgtcg ggctcgtaga ccggggtccc gtcggccagc acgaggtagg
     4441 cgacgggtcg gccgaggtcc cgctcgccgg cctccgcagt catacgtcct gcaccgggcc
     4501 ctcccgcgat cggtggtccg tccaccgtcg tggagaccgg ccgccggggc accggcctgg
     4561 ctcggccaac tccgcgcggc agtctcggca tcgcggggca gcccaccgcg gccgccggcc
     4621 gacctgtcct cccgggccga ctcccggcaa cggtattcgt gccaccggca ccgtgcgtgg
     4681 gagtagtaca acggccgacc atcgtcgtgc tacgggatcg aagtcgatcg gtctgcgagg
     4741 agatgctcat gacaacctgg actgcccccg ctcggccgga cacggactcg gactcggatg
     4801 acgtcaccgc ggctgtcgag cgggaacgac tgctcctggt gcgatgcgtc gagacggcct
     4861 acgagtcgct gcgcctgatg ccggggatgg acacctcggg acgcaccctc gtgtggttcg
     4921 ccgggcacct gctgcaggcc catcgccggg cgaccggcac ggctgagtcc gccgcccgcc
     4981 agtaaccccc ccccgcggaa actcgccttc caccctgacc tgatggagct cgatatccac
     5041 ctgtccgact tctacgccga ggcacaggcc ggacccgccg ccgggtggtc cggcacctcc
     5101 tagcgctgca gcgggaggag gcccgggcgc gacgcaattg tcgccgtcga cgtcgacgag
     5161 gggtgcgctc ctccctggtc acgcagtgcg tcgtccagcc agctgcgcag ggccggtgtc
     5221 tgcatcgcac cgtactgtcg ggcaaccacc tcacccccgc gtagcaccat gagcgtcggg
     5281 aggccgcaca cctggaagcg ctgtgacatt cggggcgcgc gcacgacctc cacctccacc
     5341 accttgatct ccccggcccg ttcggcggcc agccgttcca cgaccggtcg caccatccag
     5401 ctcagcccgc accaggtggc ccacaggtcc acgagcaccg gaatcgacac ctgctccgcc
     5461 acctcggcga actcgtcgtc gccggcacct gcgatccagg gcagggcatc gtggcagttc
     5521 ccgcacctcg gcacacccgt ggcgacggcg ggcacccggt tgcgcttccc acagcgccgg
     5581 caggtgacga tgcgcgcgcc gtcattgtct cccccgtcgt cgaggtggag cgccaggacc
     5641 agccaccgga aggcctgttg cagccgcacc aggccggggt cgtgcgacac ctcgaggacc
     5701 tcgtggttgt cgcgagccat cggctggtcc ttcccgcagg tcgttgcagg gacggtcgca
     5761 gcaacgccta cgccgtgcac cggtccccag gatgcaacct cgcccgtcgt tttcggcccg
     5821 acaggtccgt cccggacatg ggcgccgacc gctcccacgg gccgactaga gcggccgtgc
     5881 ttgggcccgt caggtcttcg cactcgacga cgacagccgg gcatggtgac cggacgcaga
     5941 cgacctcgga ggcgctccat gatcctggtg acggaggcga ccggaccggt cggtcagcgc
     6001 ctggtcgagg aactgagcga cgcgcacgcc gccacgacgg ccatggtccc cgtgcaggcg
     6061 gccgcccagg acctgccgcc cgctgtcggg tacgtcgagg ccacgctcga cgacccaccc
     6121 tccgcggagg tgctgcggga gttcgaccgg gtcttcctga cgagtcctgc gcgcgaggag
     6181 caggtggagc tcgaggtgta cttcgtcgac gccctcgtcg cggcggggca ccgaccgcac
     6241 gtcgtgaagc tcgccgcgga cgggttccag gatccggact gccgggtgcg cttcatgcgc
     6301 aaccatcgcc aggtcgccgt gcacctcgag gggaccgggc tccccgtcac ctatctggcg
     6361 ccgggtgtct acacggagta cctgcttgcg gcgacggacg ccattcggga gcagggcttg
     6421 atccccgctc ccgccggcga cgggcgtgtc gcgttcgtgg cggcgagcga tgtggccgcg
     6481 gtcggggcga gcgcactcac ctcgggcgat cccggggaca tccacgtcat cacgggcccg
     6541 gaggcgctgg gctatgccga cgtcgccgcc cgggtctccg cggtgttcgc ccggcaggtc
     6601 gactacgccg acatccccgt tgaccaggcc aggtgggaca tgcgcgagga cggcataccg
     6661 gaatggcacg ccgagggcat gatcgagctg ttcggctgga tctccgccgg agcctgcgat
     6721 gaggtcaccg acacggtccg caccgtggtc ggggaggatc cccgcccgct ggagcactgg
     6781 ctgaacgagc accgtgaagc cttcatgttc cacgcgtccg gagcctcgac ccagcgcttc
     6841 tgatcgggca actcccccgc tgtccccact gggtgggagc tgggccggtt tctggtcccg
     6901 ccaggccgac gagccgcgtg gtcggctccg gggaaggtcg cagggccgcc acacgaccag
     6961 tactgcaact agacgaaacg ggagcgactg atgggctcgg aactgcaggg caagacgatc
     7021 gccatcctgg ccaccgacgg agtcgagcgc gtggagctcg aacagcctcg gggagccctg
     7081 tacggcgcag gcgcgcgcag tgacctgctc tcgatccatc ccggcgagat ccaggcccgc
     7141 cagttcgaca tggtgtccgc tgggactttc ccggtggacc ggcaggtctc cgacgcgaac
     7201 gtcgacgact acgacgccct gctgctcccc ggcgggaccg tgaaccccga ccagctgcgc
     7261 atgaaccctg gcgccgtcga cttcgtcaag gccttcgtcg ccacgggcaa gccggtagcc
     7321 gcgatctgcc acggcccgtg gacgttggtc gaggccgacg tcgtccgcgg gcgccggctc
     7381 acctcgtggc cgagcgtgcg gaccgacctg cgcaatgcgg gagccgaggt cgtcgacgag
     7441 gaggtggtga tcgacgggca gttcaccacc agccgctcac ccgcggacct gccggcgttc
     7501 tgccgggcga tcgttgagca gttcgccggc gcgccggtgt cgcgctagcg ccgctcggac
     7561 gacccggagt gccccttcca cccggtcagt cggacgggag gggcggcagc gggaagcgga
     7621 gcgtcgcggc ggtcaactgc tcgcgcagcg gggtctccgc caccacgtgc tcgatgctcc
     7681 caccttcgtc gatcacggac tgaacgagct cgtcgatgac gtccggcgtc gtgcgcagcg
     7741 ggttgccaca gatcgggcac cgctcgcctt cgatcgccat ccacccgtcg acgtcgcaga
     7801 ccacgcctgg ccgcagctcc tcgtcctgca ccagcagcag tccgaccgca gcggtggcgc
     7861 caccccacag gcagtcggcc agaccgacgg cggcgagccc gcccgccgcg tgcgtctcca
     7921 gggtctccgc caccagccgc gcctcctccg cccgctcgta gcggtcgacg acctcctccg
     7981 cgcgcccgcg gatcgtcccg aggtcctcct cgcgagaatg catcgggaac gtcccgacga
     8041 cgcggtcacg catgttccgc ggcaactggt ccaggagttc cgggatctcg tgctcctggc
     8101 cgccgacagc aatgatgtcg aactcgcgcc ggccggcgtc ctcgaccagc cgctgcgcga
     8161 cggcgcggta gtgccgcttc gccaacagct cgtccttgtt ccacgtggcg tgctcgcgca
     8221 ttccgtacgc atagttggac ttgcgcaggt ccgggtcccg gagcttgccg cgctcgacca
     8281 tctcgtcctg gtagaggtcc cagaaccgcg cccgacggcg gtcgaccacc acgacccggc
     8341 agcggtggta ctcgtcgagc accgccacga gcggtcggac ctacggggtc gcgtcgacca
     8401 cgtgccggtc gcgaaccgca cgcggcagct cgacgttctc gaacagcccc cggcccgagc
     8461 acgagaagaa ggcgacggtc cgggggtgaa gatgctcctc cgtcacggcc tccacgatgc
     8521 gccccacgtc cccgcgcagg gacaaccggg cgttccggtc caggtcagga tccttggtca
     8581 gcaggcgcac ctcgtgcagc agctcgtcca cccggcttgg gaaggcgcgg cggtcgtcgg
     8641 ggtcgatgcg ggcgtacagc gacagcacgg gcagaccgtc gccgtccatc cgcaggatgc
     8701 ggtcgatcgt ctcgacctcg agcatcgacg tcctcctcag gtgaccggga gctcgccgag
     8761 cgtgacggtg atcgtcagcg gttccgccgc aggcgggcgc ttcaccgtga gccggacggc
     8821 ctcccccgga cggcgttcgg cgagccgcgt ctgcaggtcc tgggcggtcc ggatctcgtc
     8881 gccctcgatc cgggtgatga tgtcgcccac gcgcaggcct gccgccgccg ccggcccgtt
     8941 cgggtcgagg gcggcgacac cggcgccaat cggctgcccc gtgtcgtccg tgacggtcac
     9001 cgccgtgatc ccgacggccg cgcgccccga gttgaccacc cgcccgttgt cgacgatctg
     9061 ccgcgcgatg tcgttcgcga tttcgccggg gatcgcgaag cccaccccgg gcgccgcccc
     9121 gccgatgctc gggttgacgg cagcgagcgt ggggatgccg acgacctcgt ggttgatgtt
     9181 gacgagtgcg ccaccgctgt tgccggggtt gatcggggca ctcgtctgga tcgtctgccg
     9241 tagcacggtc ccggggaaac cgatctccgg ctccgccggc tccgggatgg tccggcccag
     9301 cgccgagacg acgccatgcg tcacgctgct ctccaacccg agcggactgc ccatcgccat
     9361 gacgaggtcg cccacccgca tgtcgcgcgc cgcgccgaag cgcgcgggca ccagcgagcc
     9421 tccgtcctgc accttgatga cggcgaggtc ctcaggcggg tgggagtaca cgagagtcgc
     9481 ctgccgcacg gtgctgctgt tcgcgaacct gacctcgaac gccttccccg taccgactac
     9541 gtgtgcgttg gtcacgatgt gcccgtcgcg gtcgaagacg atgccggagc ccaccgatcc
     9601 cggctggctg atctccacga ccgacggcag gacgtcctgg acgactgcgg cgaactcatc
     9661 cgacagctcc gccgcgaacg gcggcggagg cggaggcacc ggttgcccgg cggcggagca
     9721 cccggcgagc gcggccgtgg ccgccagcaa cagcgcgagc acgagagccg atgtccggtt
     9781 cgcaccggcg tccgagcggc gaacagaccg catcggtcca cctccttggt cccggcacga
     9841 atgccggctc ccgctcgccg gcagcaccga ccgtgccagt accgcggggc gcaagtgctc
     9901 ggcccggcag gccgaactac gggcgtccgg ccgtcctagc agggcagcac ggggagggcg
     9961 ggcggtcgct cggttccggg gccggggcgg aaggcacggc gaggaccgcg tcgacgccgg
    10021 ggggcggggc gcggggccac cggttcggac cggggagaat cggttcgtgg ccaccccaac
    10081 gcctcgggat tcggagaacg gttccccgga cggatcgcat ccggccgtcc gggacccgtc
    10141 gccgtcatcg gtgcgccgct ggcgacggcg gttggcggac gagcgcgagg aggcgaaggt
    10201 ctaccgcgac ctcgccacgc gccggaccgg ggaggagcgg cagatcctgc tgagcctggc
    10261 cgaggccgag gagcgccacg ccgcccactg ggcacggctg ctcggcgacg acgccggtcg
    10321 ggtccggcgc ggcgccgcca ggatgcgcct gctggcgttc ctggcgaggc gattcggctc
    10381 ggtgttcgtc ctggcgctcg cccagcgcgc ggagacacgg tcgccctacc ggtccgacgc
    10441 ggacgccacc gcggcgatgg cggccgacga gcgggtccac gaggaagtcg tccgcgggct
    10501 ggcggcacgc ggccgggcgc gggtctcggg aactttccgc gctgccgtat tcggtgccaa
    10561 cgacgggctg gtcagcaacc tcgccctcgt gctcggtgtc atcggtggcg gcgcgtcccc
    10621 gagcaccgtg ctgctgaccg ggttggccgg gctgctgtcc ggggcgttgt ccatgggtgc
    10681 cggggagtac gtgtccgtgc gctcgcagcg cgagctgctc gcagcgtccg ccccggacgt
    10741 ccgcgacgcg cataccgtcc tccgcgagct cgacgtcgat gccaacgagc tcgcgctcgt
    10801 gtaccgggcc cgtggtgtgg cacccgagga ggcccaccgc cgggccgcag cggtgctgcg
    10861 cagcgacgat cccaccttct ccccgctcgc gaacgggccg ggtgaggacg acggatacga
    10921 cgtggtcggc acgggcgtgg gcgccgcgct ctccagcttc accttcttcg cctcaggcgc
    10981 cgcagttccg gtgctgccgt tcctgttcgg gctcaacggc gcgcccgctc tcatcgccgc
    11041 cgggttcctg gtcgggctcg ccctgctgct caccggtgcc accgtgggcg tgctgtccgg
    11101 cggtcccccg ctacgcaggg ggctgcgaca gctcgcgatc ggggccggcg cggccgcgac
    11161 cacctacggg ctcggcctgc tcgtgggtgc cgcggtcggc tgacccacgc cgtgcggacc
    11221 cgggcacgac atgccggtgc ccgccgccgt tccggctcgc aacgatgggt cgacggacga
    11281 accagtccct gaacggagca gacctgttcc ctgcgggatc ccgtccgcac ccggcaggat
    11341 gtggaggtgg acccaccact gcccgagatc cgcatcggcg accgcgagcg gcgcgaggtg
    11401 gacgcccggc tccaggaggc gctggccgac ggcatgctga gcctcatcga gtacgacgag
    11461 cgagccgcgc aatgctgggc ggcacgcacc cgcaccgacc tcgacccact gaccagcgac
    11521 ctgccacaag cacaaccgcc cgagccgccg gtggccgccg aaaccacccc gcccaccctg
    11581 tggtatgcgc ccgccgcggg agtggcaggc cgggccgtcg gcggcctggt caccgctgcg
    11641 ctcgtcggcg ccggcatctt cgtcggcgtc caggtcctct ccgccgacga cggcttctct
    11701 gtcttcggca gccggacggt gcagctcagc ggcgaccagc agcgggccga ggtcggcatg
    11761 atgttcggca gcgtccgcgt cgtcgtgccg gatgatgtgc gcgtccgctt gtcgggcacc
    11821 gtcgtcttcg gcagcatgaa ctgcgacgag gcctgccgca ccggctcgaa cctccgcgag
    11881 gtggcggtgg aggccgacgg cgcgttcggc agcatcaaca tcatgcggca gagcgaggtc
    11941 gaccgcgagg ccagcaccat ccgcgaccgg aaccgcgacg aggactccga ggacgactag
    12001 cggcggggcg gagggccgga cagggtcacc atccccccgg aaccggcgtc gaccggtagc
    12061 ttcacgccgg tcacgaagga cgcatcgatc ccatcggtcg cctcccggcc ggttcactct
    12121 cgacggcgat gtccatgccg tcaggatcgg ctggccccgc ccgacgcggt gacggttcca
    12181 tgcgattacg tggtcatccg tgcacaacag gagcgcgcag gacagcaggg acagccgccc
    12241 gatactgcac cgttcggcat gctggatatg atcgccgccg caccagatca acggcgtcac
    12301 caaagctccc cccgcggcgc ccgggcgccc cggcgatccg tgtccgtccc gaccggtgcg
    12361 tcgtaccgcc cggccctagg gcgccggccc gcacgccgtt gccgctgctc atcacctgaa
    12421 gggcgaaccc tccatgacgc agaaaccgtt cgacttcgtg atcgtgggcg gcggaacggc
    12481 cggatgcgtg ctggccaacc gcctgaccgc cgatccgggt actcgcgtgc tggtcctcga
    12541 ggccggtcgc cccgactact ggtgggacct gccgatccag ctgccgatcg ccatgggcat
    12601 gccggtcgga agcagcgagc acgactggct ctacgagtcc gagcccgagc cgcacctgca
    12661 gtcccggcgc ctgcaccatc cgcggggcaa ggggatcggt ggctccagca ccatcaacgg
    12721 gatgatctac cagcggggcc accgggcgga cttcgaccgg tggggcgcgg agtgcggcac
    12781 gcccgagtgg gactacgccc actgcctccc ctacttccgg cggctggaga actgcctcgg
    12841 cgacgaggag gacgccttcc gcggccgcgg cggcccccac accctcgcgc gggggccggc
    12901 agacggcccc atcttcgagg cgttcttcgc ggccgcccag gaggcgggtc acgccgtgct
    12961 gcccgacatc aacggcgtgg agcaggaggg cttcgcaccg ctggatcagg gcgtgcgcgg
    13021 aggcaggcgg gagtccgcgg cgcgggcgta cctgcacccg gcacgacggc gacgcaacct
    13081 cgaggtgcgc tccgggacgg cggtcagcag gatccacgtc gagcggggcc gcgcggtcgc
    13141 ggtgcggttc aggcaggacg gcggcgccga cgaggtcgtc caggctcgcg agatcatcct
    13201 gtgctcgggg gcgatcggct cgccacagct gcttcaggtc tccggcatcg ggaacgccgg
    13261 cgagctggaa acgctgggcg tacccgtggt gcacgacctg cccggtgtgg gcgagtccct
    13321 ccaggaccac ctggctgtgc acctgcagca catctgccgg gagccggtgt ccatggcgcc
    13381 cgtccgacgc aaggcgaact ggccgcggat cgtcgcggag gcactcctgt tcggcagcgg
    13441 tccgggcagc cgcaacccga tgcaggcggg cggcttcgtc cggagcagtc ccgagcagag
    13501 ccatcccgac ctgatgttcc tgctcgcgcc cctggcgatg catagcgccg agcgctcgat
    13561 cccggtcaac gagcacgggt accaggtgca cgtgggagtg atgcgctccg acgccagggg
    13621 atcggtgaag atcacctcgc cggtggcgtc ccggcatccg gcgatccagc tgaatttcct
    13681 gtccgctccc gacgaccggc agcgctggat cgacgccgtg cgcatcgggc gggagctgct
    13741 ggcgcagccg gcgatggacc gcctcaacgg gggcgagagc ctccccgggc ccggcgtgcg
    13801 ctccgacgac gaggtcatcg agtgggtcac ccgcaccgcc cgggccgggt tgcacctcgc
    13861 atgcagcacc cgcatgggca tggacgagcg ggccgtggtg gacccggtga cgctccgggt
    13921 gcgcggcctc gacggtctgc gcgtcgtcga cgctgcgatc atgccctccc tcaccaacgc
    13981 caacacctac ggcccggtgc tgatgctcgc agagaaggcg gccgacgtga tcctcggcaa
    14041 cacccccctc gctcccgagt acccggccgc accggctcgg gtggcggcgc agcccccggt
    14101 cgcccacccc gcgggggccg cctcggtgga ccgctgaggt gcccgccggc gcccatcggt
    14161 ccgccgggcg ggggccgcac ccgggccgat cacatcggtc agggtcggct gccttagggt
    14221 ccgggcgcat gaggagtgcg ctggcgagtt ggcggtcccc ccagcaccgc gacggactgg
    14281 cgctcgtact cagctcggcg ctgtcctcgg gcatcgggct cctctactgg gtcgtggccg
    14341 cccggttgtt cgcccctgac gtcgtcggag tgaattcgac gctggtctcg acgctgacgc
    14401 tgctcggcag cgcggcgcag ctcaacctgg gtagcgcgct cctccgcttc gttccggtgg
    14461 ccggtcggtc ggcgcggtcg ttggtgctgt cctgctacgc ggtgggagcc gggacggcgt
    14521 gcgcgaccgg tgccgtgttc gccctcggtg ctgcgtggtg ggcaccggat ttgctcgagt
    14581 cggtgggcca tggccagctg atcgccttct tcgcgctctc gacgcccgtg tgggcggtat
    14641 tcgtcatgca ggactacgtg ctcaccgcga tcaagcgggc gacgctcgtg cccgtggaga
    14701 acctcgtctt cgcgctgctg aagatcgcgt tgctgatggc aggcggggtg gtcgcgttct
    14761 acggaacgat cgccgtgtcg tgggcggcgg ccaccgccgt cctggtgctc gtggtcaccg
    14821 tttggctggt gcgcatcctt ccggcctacg ggatcgccac cgcggcctcg gcagtgtcgt
    14881 tcagggcgca gacgatcgtc ggattcgtgt ccgcggactg ggccggaacg ctcttctccc
    14941 tggccgtcaa cttcggcctg ccgctgctcg tgctggcgcg gctcgacgcc gactccgccg
    15001 ctacgttcgg ggtggcgtgg gcagtcgcct tcgggctcta cctggtctcc aacgggatgg
    15061 gccagtcgct ggtggcgcac gtgtcagccg acccggatgc cctggacgcg gcctgccgca
    15121 gcatggtcac caaggcgatg acactgctgg tgccggcagt ggtggtgatc gttcccggtg
    15181 cgggactgat cctctcggtc ttcggcgagc actacgcggc caccggcacc ggcctgctcg
    15241 cgctggccgc actgtccgcc ctgccgaacg tcgtgctcct cgcgacggtg aacgccgcca
    15301 gggttctgca gcgcaagagc atccagttcg gcgttccggc ggctctcgcg ttcatcgtga
    15361 tcccgctcgc ctgggtgttg atgccgttcc tgggcctgac cggcgtcggg gtcgccctgc
    15421 tcgtcgggca gacgatggtg gccgtcgtca tcctgctggt ccgctggacg ggtccgcgct
    15481 tcgacacccg cggactcccg cccaccgtcg ctgttcgttc ctcgacggcg gcgagcagcg
    15541 gttcctgaac cgccgggcgg gccgccagcg cccgccgtgt gccgccggga aaacatcctg
    15601 gcgtcgcaaa gcgcacccgt aaccccctgc gcaggggttc tctgcacctc gatcccggaa
    15661 aactcgacat tccggctacg tcattcgccg gacacgccgg gacggttcca tttgcctgaa
    15721 tcgtcccgga aacgtcagta atggagccgt cacaccaccc caggccgtga tcagctgtta
    15781 gcaaagcggc cgactccgga tcaccagccg ctcatgtgcg gttgcaaaag tctccccagc
    15841 cgcgggctgc cgcggtgtcg tcgaccggta ggggctgcgg gagtgcggga atgtcatcgg
    15901 taacgtctgc tccggtgtcc acgccgccac ccaccaccgc ccggcacgtg cgaacaccca
    15961 cttcccgact ccgtttcggc gcccgcgaac gccacgacta tctcgtgttc ctcgctctcg
    16021 ccgcaccgaa catcctgctg atcgtcgcgt tcagctaccg cccgctgctc agcaacatct
    16081 actactcgac gctcaactgg acgctgggcg ccgccacggc ggtgccggtc gggttcgaca
    16141 actacgtccg gttcttcacc tccccggaca ccgccgagat cgcccgggtg acggccgtct
    16201 tcaccgtcgc caccgtcggg gggtcgatgc tgctgggcct gctgatcgcg ctggtgctga
    16261 accgcaaggt ccgcggcgcc gggttcgccc gggccgcggt gttctcgccg ttcgtactgt
    16321 cgggcgtcgg cgtgggactg atctgggtgt tcatgttcga ccccaccatc ggcgtgatag
    16381 cggcagtgct gcgcacgatg gacctgccga gtccgcagtg gttcaacgat ccgtcgctca
    16441 gcctcgtgat ggtgatcgtc gtctacgtct ggaagaacat ggggtacgcg gcggtgatct
    16501 gcctggcggg gctgcaggcc gtcccgcggg accttctcga ggccgccgcg atcgacgggg
    16561 ccggccggct gcggacgttc cggtcgatca tcctgccgat gctctccccg acgacgttct
    16621 tcctgctgat cacgagcgtc ctcaactccc tgcaggcctt cgacatcatc cggatcatga
    16681 ccccgctcgg ccggggcacg accaccttga tctacgacgc ctatctgcag gcgttcggcg
    16741 gctacaaccg cgccggatac tccgccaccg tgtcgacggt cctgttcggc ctgctggtgc
    16801 tgttcaccgc gatccagatg ctggtcctcg agcgacgggt gcactaccga tgaccatgtc
    16861 ggcgacgggc cccgagcagg acacccgcac ccatcccctc gccccacgca acctcgtcgc
    16921 cgcggccgtc ggctccttca tcccgctcgt gctcgccgtc gcggtcgtcg cgctgccgct
    16981 ggtgtggatg ctgctctcgt cgttcaagac gccgggggag gtgctgaccc gggacctctc
    17041 ggtcctgccc cacagcctgg ctccggacaa ctacctgcag gccgcccgcg aggttccgtt
    17101 cgtccggctc ttcgtgaact cggcgatcgt caccgtcatc ggagcgggca tcaaggtgct
    17161 actggcgatc ttcacggcct acgcgctggt cttcgtgcgg ttcccggcca agaagctgat
    17221 cttcgccgcg atcctggtga cgctcatggt gccgccgcag gtgtcgctgc tcccgaacta
    17281 catgctcgtc tccggcctcg ggggcgccga cacctactgg ggaatcatcc ttcccggcct
    17341 gggcacgggc ttcgggacgt tcctcctgcg gcagcacttc atgacgctgc cgatctcgat
    17401 cctcgaggcg gccgagatcg acggggcggg ccactggcgc cggctcaccc gcatcgtcat
    17461 cccgatctcc gggccgagca tcgtgaccgt ggcgctggtc agcatcgtgt tcgagtggaa
    17521 cgactacctg tggccactgg tcatcgtcac cgacccgtcg atgatgaccc tgccggtggg
    17581 actgaccctg ctggcgaaca ccgaaagcga cgcactggcg tacggggtga tgatggccgg
    17641 atccgtcgcc gtcatcgtgc cggtcctcat cgtgttcgcc tcgctccagc gctacgtcat
    17701 cgccgggctc acccagggcg ccgtgaagga ctgaggagca cctccccgcc cgacccgtct
    17761 gtctcccccg catcatctcc acacatcaga aggaccttcc atgaccgctc ggttccccga
    17821 cacctccccg cacgcccggc tcgtcgcacc gaacctgcac cgtcgcggct tcctccgcct
    17881 cgcctcggca gcgggcctga ctgcggctct ggccgcatgc ggtgggccct cgacgtcggg
    17941 ctccggagcg tcaccggcgt cgtcggcgcc ggcggacatc gactacacgg gcgtgcagcc
    18001 ggcagcccag gtcaccatgt ggtcaagcaa ccccggctcg tcccaggctg tgacccagca
    18061 gatcatcgac gcgttccacg cctcacagcc cgacatcaag gtgaacctgg tgacggcggg
    18121 caaggactat gaggagatcg cgcagaagtt ccagaccgcc cagaccggcg gaacactgcc
    18181 cgacctcatc gtgctgtcgg acgtgtggtg gttccgctac tacatgctgg gatccatcgt
    18241 cccgctcgac tcggcgatca aggccgtcgg catcgagacc ggcgactacc aggaccagct
    18301 gctggccgac taccagtacg caggcgggca gtgggcgctc ccgtgggcgc ggtcgacgcc
    18361 gctcttctac tacaacaagg cgcactgggc ggcggccggg ctgcccgacc gcgccccgac
    18421 gacgtgggcc gagttcaacg agtgggcgcc gaagctgcag gccgcgggca ccggcgtcca
    18481 gcacgctttc cagctgccgg cgctcgcggg ctacgccgga tggaccttcc agaacaacct
    18541 ctggggctgg ggtggcgcct ggagcaagga ctgggacgtc acctccgact ccgccgagtc
    18601 ggtcgccgcg atgcagttcc tccaggacgc ggtgtacaag ggccggtggg ccggggtgtc
    18661 cagcaacgac tccgccaacg acctgtccgc cggagcagtc agcgcgacca tgagctcgac
    18721 gggcagcctg gtgggcatcc tcaagaccgc cacgttcgac gtgggtgccg gtccccttcc
    18781 cggcggcccc gctgccacct cccccgtgtg cccgaccggt ggtgccggcc tcgggatccc
    18841 caaggccatc ccgaaggaga accagctggc ggcggcgacc ttcctcaagt tcctcacctc
    18901 ccccgagaac accgtcacct tcgccggcgc caccgggtac atgcccatac gcaagtcggc
    18961 ggacatctcc gcgctcgtgg cgaaggcccc gcagagcaag gtcgcgatcg accagctgac
    19021 ggtcacccgc acgcaggacc gggccagggt gttcttcccc ggtgccgacc aggagatggc
    19081 caagtcgtgc gccaacatcc tgacgcagca ggccgacgtg cactcggaga tgaccgcact
    19141 gaaggcgacc ctgaccggca tctacgacca ggacgtcaag ccgaatctgt aggtcccctg
    19201 ccaccgtgtg ggcggtggac ctcgtactac cctctgcctg ctgtggaacc cgctccctcg
    19261 atcgacgccg ccacctcgcc gttcgaccgg cgagtgtggc gggtcgtcgg tgatccgctg
    19321 gtcagaggcg cacgcacggc cgtgacggcg ctgccgctgg cggggatcgg agtggcggtc
    19381 aaggatttga tcgccgtggc cgggcagccg gtcggtgcgg gcaaccccgc atggctggcc
    19441 gagcagcagc cgcaacaccg ctccgcgccc gcactcgagg cgctgctcgc ggcgggcgcc
    19501 gacgtggtcg ggatcgcccg aacggacgag ttcgcctaca gcctcgcggg caccaacgtc
    19561 cactacggca cgccgccgaa ccctgccgca ccgcgtgcga tcagcggcgg ttccaccagc
    19621 ggtccggcga gcgccgtcgc gctcggccaa gctgcggtcg gactcggcac cgacaccggg
    19681 ggctcgatcc ggatcccggc ctcctatcag gggctggtcg ggatccgcac cacccacggc
    19741 gccatcgaca cggccggcgt gctgccgctc gctccggcgt tcgacacggt cggttggctc
    19801 acccgcgaca ctccgacggc gctgcgcgtc gcgggcgtgc tactcgacgg gacccaggac
    19861 cgggctctgg acgccgcccg caccgtcgtg ctcccggcgg cacagcggtg cgcctccccc
    19921 gacgtctccc tcgcgtgtga cgtcctgcgc gaccgactcg cagctggcgg cgtcctcccg
    19981 ccggtgcagg acgacgacgt ccccgccgac gttctccaga ggtggctcgc cgcgttccgc
    20041 accgtgcagg cgtaccaggc ctggacggct cacggggcct gggtacgcac acaccccggc
    20101 gcgctcggcg atgacatcgc tgagcggttc gccgtggcgg cacaggtcac ggaggccgag
    20161 gccgagcgcg cagccggggt ggccgagcag gcgcgggcgc agttgcagcg gctgctcgcg
    20221 aacgcggtgc tggtgctgcc cagcagcgca ggcggtgcgc tcccgctgca cgccgccgcc
    20281 accgaggtgg aggcggagcg cgcgggaacg ttgcagctga cgtgtgtggc cggcctggca
    20341 ggcgcaccgg cggtgtcggt gcctgccctg cagacctccg acgggcgtcc ggccggattc
    20401 tgcctggtcg gcgccccggg caccgaccgc gacctgctgc gccttgccca ccggatcgct
    20461 ctgtggagcg ggacgttcag tccgtagacg tcctccggtc gggaccggcg tcggcgagag
    20521 cgtcgaccgc tccccgcagg cgcagcgccc acacgacgag gacgaccccg aagacgaggg
    20581 agaacgctcc gaccagcaac aggagcgtga tcagcccggc gcccggctgc gcgagcagga
    20641 cgatgccgaa gagcacgctg acgacaccgc ttgccaggct ccagccccac gtcgacgcgc
    20701 cccgcctgcg catcgcaaac gactcgatga tctgtgccac gccgttgata atgctccaga
    20761 aagcgatcac gaaaaggatg gccagcaccg tcacacccgg ccaggcgaaa gccacgatgc
    20821 cggcgatcag cgaaacgact ccctggacga cgtgccagcc ccagtgcggt tcctccttgc
    20881 gatgccgcac cccggccaca atcgccgtga tgccatccaa gatcgcatac gctccgaaga
    20941 cgaacaccag ggcgagcagc gcggtacccg gcgcgaagag cgcgaacagt ccgaagagga
    21001 tcgccaggat cccgcggagg agcacgagcc accacaccgc gcgcacagcc gacttcgctt
    21061 cccacgtcgt catgatcgcc tcctatcgac ggcgccggac gtcggcggtc gccaggtgaa
    21121 ttgtggaaat ggcaacgcta ccggtagaac agccgccgtc gatcccctga tcgggcagca
    21181 tgatttgctc cggataccgc ggaacgcaca gctcggtgtt cggcgtgcgt agtcagcggg
    21241 gcgctcacac ccccagcacg atggacgagg cccacagggc gacggtggcg gcggcgagca
    21301 ccggcggcac ggtgaccgcg ccgagcgcgt ggaactgtcc ggaccgcggc cgatgcgcag
    21361 cgggtagcaa ccggcgccac agcaacgtgg ccagcgagcc gacgtaggtc aggttcggac
    21421 cgatgttgac gccgagcaga acagccagaa cagcggtggg atgacccgcg acgaccggca
    21481 gcagtaccag cgtggcaggc aggttgttga cgatgttcgc cagaccggca gcgacgaagg
    21541 cgagcaccaa caagatcagc agctggtccc cgtcaggcag gatgccgacc agtgtcgagc
    21601 cgagtccgtg gcgggtgacg gcgtccacga ccacaccgag cgcgacgacg aagacgcaga
    21661 agcccgggtt ggcctgcagt gcgatctcgc gcggcgtgac ctcgcgacgc agtagccgcg
    21721 gaacgccgag cagcagcacg ccgccgaagg cagccagggc cggtgtgacg ccgagggcgg
    21781 aggtgaccac caacccgacc accgtgaccc cgaggaccgt catggcgtac cgcggtgcag
    21841 ggtcgtgcgt gtcgacctcg gtgccgccac cgtgcaggtc gcgccggaag aaggcgcgca
    21901 gggcgctcca ctcgagcagg caggtcagca gccacggcag cgtcatcaac gcggcgaacc
    21961 gcccgaagga cagcccggac gccgtgaacg ccaacaggtt ggtcaggttc gacacaggta
    22021 gcagcagcga tccggcgttg gccaggtgca ggcaggcgta gaggtccgga cgcgcttcga
    22081 cgtcgacacg tcgagcggtg cacagaacga ccggggtcaa caacaccacc gtggcgtcga
    22141 gtgtcagcac ggcggtgacc acggcagcca gcgcgacggt gagggcgagc agccgggtcg
    22201 ggcggcccgc ggctgcacgc ccggccagcg tggcgaggtg agcgaagaca ccggcaccgg
    22261 cacagaggtg cccgaaggcg aggatcgcgg ccaggaacaa cacggtcgga agcagttggc
    22321 ggaaggtggc cagcgcggcg tccatcggca ccaggccgac acccacgacg agtgctgcgg
    22381 ccgggacacc gaccacggcc tcgttccccc cgaacggccg ctggatcgcc gcgagcagca
    22441 caagtgccag gaccacgacc gcgacgagct ctcccattgc ctcccagcat ggtgtcgctc
    22501 agtcggatga cgggccgccc gtgccgaacg tcatgcggtg tcagggactt ccgccgggcg
    22561 gggtttcacc gcctcggaca ccaaccgtcg gtagatgcgg tcgggagtca ggggaaggtg
    22621 ccggaagcgg actccggtgg catcggccac cgcgttggcc agtgcgggcg ccacggggtt
    22681 gatgcagcac tcggcgatgc ccttggaacc catcggtccc accgaatccg tggacggcac
    22741 gcagatgacg tccgtgcggg gtgcatccgc gtaggtgggg atgcggtagt tgcggagatt
    22801 gggattgcgc atccggccct cgtcgtcgag ctggaagctc tccgtgagca cgaagccgag
    22861 gccttggacg ac
//
LOCUS       DASRSC010000085         2075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4777177,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000085 DASRSC010000000
VERSION     DASRSC010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4777177"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="VFE39_04375"
     CDS             <1..645
                     /locus_tag="VFE39_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein"
                     /protein_id="HET6257869.1"
                     /translation="PAAGATGGGVSLSKVTLTKSAPSVSLTKRGGTGGQLRVNLQWSA
                     GEQQGRGGLFGKRRGSSIDLDLACLWEFSDGSKGVVQALGNAFQAPYQGPPIIRLDGD
                     DRSGSAAGGENMFIDLSRVDEIRRILVFAFIYEGTPNWAGADGVVTLYPASGPEIEVR
                     LDEHDTGSPTCVIAMLENQGGEMVVRREVRYIRGGQADVDDAYGWGMEWARGRK"
     gene            711..1874
                     /locus_tag="VFE39_04380"
     CDS             711..1874
                     /locus_tag="VFE39_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HET6257870.1"
                     /translation="MRHFGYLSASETDALFEARPEPFRRDSGHDLLSVALGATLYMPA
                     TRPALAADITKQAAAGVTSVVACLEDSIADEQVAEGEANLVAALTELHEHPQGEPPIL
                     FVRVRHAAQIPELVARLGPAAAVLDGFVLPKFTAATGTEYLHALDDADRMAGTALLAM
                     PVIESGAVLYTETRVDELTRLRALLHRDRRRILAVRLGATDLCALYGLRRSPDLSIYD
                     VKVVSSLISDVVNVLGRADGSGFTVTGPVWEYYAGVERIFKPQLRFSPFAEHNEPELR
                     GRLVGRAIDGLIREVSLDRANGLTGKTAIHPSHVAVVNALSVVSFEEHRDASDVLQAG
                     RAGGVLASGHRNKMNEIGPHTAWARLTMLRARAFGVAADNVSFVDLLAASLPR"
     gene            1871..>2075
                     /locus_tag="VFE39_04385"
     CDS             1871..>2075
                     /locus_tag="VFE39_04385"
                     /inference="COORDINATES: protein motif:HMM:NF026947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HET6257871.1"
                     /translation="MSEVASESRTPPVERLGVRLRPTGPGVDPTVLLGLALRRNPRRA
                     QLLVSRVLGKHVPTDPRLVRAAGL"
BASE COUNT          289 a          742 c          750 g          294 t
ORIGIN      
        1 cccgcggccg gcgcgaccgg cggaggggtg tcgctgtcga aggtcaccct gacgaagtcc
       61 gccccgagcg tctcgctcac caagcggggt ggcaccggcg ggcagctgcg ggtcaatctg
      121 cagtggagcg cgggcgagca gcagggccgc ggcggcctgt tcggcaagcg gcgcggatcg
      181 tcgatcgacc tggacctggc ctgtctgtgg gagttcagcg acggcagcaa gggcgtggtg
      241 caggcgctgg gcaacgcgtt ccaggcgccg taccagggcc cgccgatcat ccggctcgac
      301 ggcgacgacc gctccggctc cgcggcaggc ggcgagaaca tgttcatcga cctgtcccgg
      361 gtcgacgaga tccgccggat cctcgtgttc gcgttcatct acgagggcac tccgaactgg
      421 gcgggcgcgg acggcgtcgt cacgctgtac ccggcttcgg gacccgagat cgaggtccgg
      481 ctcgacgagc acgacaccgg ttctccgacg tgcgtgatcg ccatgctgga gaaccagggc
      541 ggtgagatgg tggtgcgccg cgaggtgcgc tacatccgcg gcgggcaggc agacgtcgac
      601 gacgcgtacg gctgggggat ggagtgggcg cgcgggcgca agtagcccgc accgaccgac
      661 cgcgctcccg tacacccctg gaccggacga ctctgagagg gcctgcaccc gtgcggcatt
      721 tcgggtacct gagcgcgtcc gagacggacg ccctgttcga ggcgcgaccg gagccgtttc
      781 gtcgcgacag cgggcatgac ctgctctcgg tcgcgctcgg cgccacgctc tacatgccgg
      841 cgacccggcc cgccctcgcc gccgacatca ccaagcaggc ggccgccggc gtcacgtcgg
      901 tggtcgcctg cctggaggac tcgatcgcgg acgagcaggt ggccgagggc gaggccaacc
      961 tcgtggccgc cctgacggag ctgcacgagc atccgcaggg tgagccacca atcctgttcg
     1021 tccgcgtccg gcatgcggcg cagatcccgg agctggtggc gcggctcggg ccggccgcgg
     1081 cggtcctgga cggcttcgtc ctgccgaagt tcaccgcggc caccggcacc gagtacctgc
     1141 acgccctcga cgacgccgac cgcatggccg gcaccgcgct gctggcgatg cccgtgatcg
     1201 agtccggcgc cgtgctctac accgagaccc gcgtcgacga gctgacccgc ctgcgcgccc
     1261 tgctacaccg tgaccggcgc cggatcctgg cggtacgcct gggagccacc gatctgtgcg
     1321 ctctctacgg gttgcgccgc tcgccggacc tgtcgatcta cgacgtcaag gtcgtcagca
     1381 gcctgatctc cgacgtcgtc aacgtcctgg gccgggccga cggcagcggg ttcaccgtca
     1441 ccggcccggt gtgggagtac tacgccggcg tggaacggat cttcaaaccg cagctgcggt
     1501 tctccccgtt cgccgagcac aacgagccgg agctgcgcgg ccggctcgtc ggcagggcga
     1561 tcgacgggct catccgcgag gtctcgctcg acagggcgaa cggtctgaca ggcaagacag
     1621 ccatccaccc gagccacgtc gccgtcgtca acgcgctgtc ggtcgtctcg ttcgaggagc
     1681 accgcgatgc ctccgacgtg ctgcaggcgg ggcgggcggg cggcgtgctg gcgtcgggcc
     1741 accgcaacaa gatgaacgag atcggtcccc acaccgcgtg ggcccggctg acgatgttgc
     1801 gggcacgggc gttcggggtg gccgccgaca acgtctcgtt cgtcgacctg ctggcagcga
     1861 gcctgccccg gtgagcgagg tcgcgagcga gtcgcgcaca cctcccgtcg agcggctcgg
     1921 cgtccggttg cggcccaccg gcccgggggt cgatcccaca gtgctgctcg gcctggcact
     1981 gcgccgcaat ccgcgccgcg cgcagctgct cgtatcgcgg gtgctcggca agcacgtgcc
     2041 caccgacccc cggctggttc gcgccgccgg gctcc
//
LOCUS       DASRSC010000086         9323 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1991611,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000086 DASRSC010000000
VERSION     DASRSC010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9323
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1991611"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1224..1297)
                     /locus_tag="VFE39_04390"
     tRNA            complement(1224..1297)
                     /locus_tag="VFE39_04390"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1261..1263),aa:Pro,seq:cgg)
     gene            complement(1322..2266)
                     /locus_tag="VFE39_04395"
     CDS             complement(1322..2266)
                     /locus_tag="VFE39_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6257872.1"
                     /translation="MNSWARIALGATTTGAAAVAYAAGIERRRWTLREATLPVLAEGA
                     RPLRVLHVSDLHLTPRQHSKQRWVAELAALEPDLVVNTGDNLAHPRAVPAAVAALAPL
                     LQRPGLFVFGSNDYFGPTPKNPARYLVKSSRRRIHGEPLPWRDLRAAMVEHGWSDATH
                     AQVKLTVAGVTVTAAGVDDPHLGLDRYDRVAGPPGGEPGLRLGLTHSPEPRVLDRFAA
                     DGYDLVLAGHTHGGQLRMPGVGALVTNCGLDRSRARGTSRWGTHTWLHVSAGLGTSPY
                     APVRFACPPEASLLTLVPRPSVVGAPRTTPASTAVDVG"
     gene            2380..2850
                     /locus_tag="VFE39_04400"
     CDS             2380..2850
                     /locus_tag="VFE39_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HET6257873.1"
                     /translation="MSTLKEQLRADLTAAMKARDELVKGTLRMTLTAIGNAEVAGAEA
                     RELDDAEVVKVITKEAKKRAESAEAFAAAGREELAAQERAEGEVLARYLPAQLSDEEL
                     AVIARTAVDDTAAELGEQPGPRQMGQVMRKASAATAGRADGGRVAAAVKALLVS"
     gene            complement(2913..5645)
                     /gene="topA"
                     /locus_tag="VFE39_04405"
     CDS             complement(2913..5645)
                     /gene="topA"
                     /locus_tag="VFE39_04405"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET6257874.1"
                     /translation="MIVESGTKAKKIQQYLGNNYVVEASVGHIRDLPRGAADVPAKYK
                     GEAWARLGVDVDNQFTPLYIITPEKKGKVAELKDALKSVDELLLATDHDREGEAIAWH
                     LLETLKPTVPVRRMVFHEITESAIRAAVENLRDLDHDLVDAQETRRILDRLYGYEVSP
                     VLWKKVMPKLSAGRVQSVATRIIVQRERERMAFVSASYWDIAAQLDAGADASPRQFPA
                     RLVAVDGTRVATGRDFGPDGRLKGADGSPNRADGKETARPLDEASARRLADALQGRDL
                     AVESVESRPYTRKPYAPFMTSTLQQEAGRKLRFSADRTMRSAQRLYENGYITYLRTDS
                     TTLSESAIQAARSQARELYGDAYVPAQPRQYTRKVKNAQEAHEAIRPAGDTFRTPGEI
                     AREVDGDDFRLYELIWQRTVASQMADARGTTVSVRIGGTAATGEAVTFAASGRTITFP
                     GFLKAYVETVDDQAGGEADDAESRLPELTQGQALTAARLTPEGHSTNPPSRFTEPSLI
                     KTLEDLGIGRPSTYASIIKTIQDRGYVWKKGQALVPSWIAFAVVGLLEHHFGRLVDYD
                     FTAAMEDELDSIAAGTQGRTDWLSGFYFGADQGSAGSVARSGGLKKLVGVNLEEIDAR
                     EINSVPVFDDVVVRVGRYGPYLERIRDGQSQRANLPDDLPPDELTPEVAEQLFSVPQE
                     GRSLGVDPMTGHEIVAKEGRYGPYVTEILPEPAEPETPTNGAKKKASAKAKPRTGSLF
                     KGMSTESVTLEEALRLLSLPRLVGTDPESGEEITAQNGRYGPYLKKGTDSRSLTDEEQ
                     LFTVTLDEALEIYKQPKQRGRRTAAATPPLREMGNDAATGKPMVIKDGRFGPYVTDGE
                     TNASLRKGDSIEQLTDERASELLAERRARGPAPKKTPAKTPAKRAPARKKSPTK"
     gene            complement(6023..6616)
                     /locus_tag="VFE39_04410"
     CDS             complement(6023..6616)
                     /locus_tag="VFE39_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257875.1"
                     /translation="MSQLSLFSADARPPRVSDLAGLLCGPGQIVRFGAGDQARLSIVL
                     ADPARAPALAAASAAAGITMEAVQTEGGTTALRSAFRRDLVGLARAWTRGAVKTVPPG
                     WQLDGPALRLWALAAGRPDERGGYLLALDPHAPETHLPLVAAATRAGIPPARVGRGQA
                     LRISGTRRVQRLVELVGPVPPRADTADWPRYWGRNAS"
     gene            complement(6772..7413)
                     /locus_tag="VFE39_04415"
     CDS             complement(6772..7413)
                     /locus_tag="VFE39_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257876.1"
                     /translation="MTRTTRPAQISATLIAAVRIAAVPAVAAVLAVLAGCTTAVPGSP
                     AADPAPTPTEGPGSDPVVWADHVCGAVLSFAKPATTPPVLAPAADLPTVQRAVSDYLG
                     AVLIGVQQGRTQLAAVGRSPLQGGAEAVGRAESALEFLEQDFTGAKNAVDTADPNNPD
                     SVKAALTRAQSTFDAITPPNPLGDLSSTPRLQRAAERAAQCQQLSALSTAAPR"
     gene            complement(7499..7954)
                     /locus_tag="VFE39_04420"
     CDS             complement(7499..7954)
                     /locus_tag="VFE39_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257877.1"
                     /translation="MQWVDGVCGELLPFVRAAGTPPQLTSAADPTALVKSISDYLAQG
                     AGAADSAITGMARVGPSPVSGGDQVVTRLTETLTKFRTSFQNAKTQVDAVDTTDQQAL
                     ITELPKAVAPLQELANMPDPTVDLQTNPELQRASTQAPNCQQIQRQVGG"
     gene            complement(8169..8723)
                     /locus_tag="VFE39_04425"
     CDS             complement(8169..8723)
                     /locus_tag="VFE39_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257878.1"
                     /translation="MGTRRFTCTLASAFVLVIAAGCGGTGDADSDAVAWTDQVCGALS
                     GFARAATSQPQVGGADPVSTVRGLGDYFSATATALQGSISELDAVGPSPVEGGDEYVA
                     RLKGALTEIRTSFDTARGQLAGVDTSSVQAMSVALPAAVAPLQELRNLADPTDGLRAT
                     DELRAAADKAANCQQVRSATSPKR"
     gene            complement(8859..>9323)
                     /locus_tag="VFE39_04430"
     CDS             complement(8859..>9323)
                     /locus_tag="VFE39_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HET6257879.1"
                     /translation="PGLLAIFAPIAVGFGLGVGPLAGYLAGAIACGTLMAVFLANSGG
                     AWDNAKKLVEDGNHGGKGSPAHEATIIGDTVGDPFKDTAGPSINPLLKVMNLVSVLIA
                     PAVVTFSIGASASAPLRIAIALVAVAIIVAAVVVSKRRSTSIADTPAASSVG"
BASE COUNT         1287 a         3171 c         3470 g         1395 t
ORIGIN      
        1 ggagtccggg tacggcgacg aggggggcgt ggtcgtccag cagtgggagg gtgcacacga
       61 gatcaggatg ccggaaccgg gccgaggtcg caggtgcagg ttgcgcgtgc ccaccgcctg
      121 cccttggtcc gcaggggaca aacgtacccc cgatcatggc ggtgaatctt accgcaggtc
      181 gcgcccgcga actcatcagg cgggaatcgt cggagaaatg ctggaagcgg tcggccgcgg
      241 ggcaggcgaa tgtcgcgcgc accgccgagc tgacgccgtc cgcgcccgtg cagaggatct
      301 gcggccggca gggatggcct gtcacgtcac aaatcccact ctgccggcgc atgtccggcg
      361 gtccataggc gcacagcgcc tggagccgcc aagcggaggg atggacgcgc atgccgtccg
      421 gcgcagttcg acccgtccgc gggcagggga cggcttaagg cgtagagaaa gttctcaccg
      481 cacctcacgt acgccatggc cgttctgcca ggctgattgc cgtgacagat ggtcacccgc
      541 atggttccgg gctcctcgag cggatcgcga tgacggtcga ttgcccggag ccggatgtca
      601 tggcggtttc tccatcgctc tgtgcggggg taggtgaccc gccgcgtgcc cggtgagccc
      661 ggagactcgg tcggggtcga gtgcgagtca gcggattgaa gcccataggc acagcacatg
      721 cctggagtgg cccatgcgca accctcagtc gagctccaag aaccgaacgg gcgggattct
      781 cagcgccgtc acgtcacgtc ccggtggggc cgggtctccg cacggcggcc cagggcattg
      841 gtgacgagag gacgatcgat gttgcgggct tgccgagtcc gttgaggtcc tcgatgagtg
      901 gttcgaggtc gccgatccgg ggtgcgacga cgcggaggac gtggtcgttg gtgccggtga
      961 agcgatggtc ctccaggatg atgttctcga ggcgtcggac ccgggccgcg acggctgtgc
     1021 tggcggctgc cttctccgcg gtggtggcgg tgctggtcgg gcagcggcaa cggcacgatc
     1081 ttgagacgtg aaggtcgacg tgaatggcac tgtcccggtc gctggcgggc ctgcctggtc
     1141 tgtggcaagc agatccggca ggcaacgacg aacggcctgg ttggaagctc cgcgtggagc
     1201 ctctgaccag gccgtgtggt ttgtcggggt gacaggattt gaacctgcga cctcttcgtc
     1261 ccgaacgaag cgcgctacca agctgcgcca caccccgatg gcccgtgagc catggacaac
     1321 tctagccgac gtccacagcc gtcgacgcgg gggtggtgcg tggcgcaccg acgacggacg
     1381 ggcggggtac gagcgtcagc aggctggcct ccggggggca ggcgaaccgt acgggggcat
     1441 acggcgaggt gcccagaccc gccgacacgt gcagccaggt gtgggtgccc cagcgggacg
     1501 tgccacgggc ccgggaccgg tcgagcccgc agttggtgac gagcgcgcct acccccggca
     1561 tgcgcagctg cccgccgtgg gtgtgaccgg ccagcaccag gtcgtagccg tcggcagcga
     1621 agcggtcgag gacgcggggc tcgggggagt gggtgaggcc cagccgcagg ccgggctctc
     1681 cgccgggagg gcccgcgacg cggtcgtagc ggtccaggcc aaggtgcggg tcatcaacac
     1741 cggctgcggt gaccgtgacg ccggccacgg tgagcttcac ctgcgcgtgg gtggcgtcgg
     1801 accagccgtg ctccaccatc gcggcccgca ggtcccgcca cggcagcggc tcaccgtgga
     1861 tgcgccgcct gctgctcttg acgaggtagc gggccgggtt cttgggggtg ggcccgaaat
     1921 agtcgttgct gccgaagacg aacagccccg ggcgctgcag gagcggggcg agggcggcga
     1981 cggcagcggg caccgcgcga gggtgagcga ggttgtcgcc ggtgttcacg acgaggtcgg
     2041 gttcgagcgc ggccagctcc gccacccagc gctgcttgct gtgctggcgc ggggtgaggt
     2101 gcaggtcgga cacgtgcagg acgcgcagcg ggcgggcacc ctcggcgaga accggcagtg
     2161 tcgcctcccg cagcgtccac ctgcgccgct cgatgcccgc cgcgtacgcg accgcggccg
     2221 ctcccgtcgt ggtcgcgccg agtgcgatcc gagcccagct gttcacccgg ccagcgtacg
     2281 gccgaggccc acgtgtccgc gatcccctgg cgatcaccac cggcttccga ccgtgccgcg
     2341 gcggcgtcga acgcggcaga tgcattagcg tgcaccgtcg tgagcacctt gaaggagcag
     2401 ctgcgggccg acctgaccgc cgccatgaag gctcgcgacg agctggtgaa aggcactctg
     2461 cggatgacgt tgacggcgat cggcaacgcc gaggtcgccg gtgccgaggc tcgtgagctc
     2521 gacgacgccg aggtggtcaa ggtgatcacg aaggaggcca agaagcgggc cgagtcggcc
     2581 gaggcgttcg ccgccgccgg tcgcgaggag ctggccgcgc aggagcgcgc ggagggcgag
     2641 gtgctcgccc gctacctgcc ggcgcagctc tccgacgagg agctcgctgt catcgcgcgc
     2701 accgcggtcg acgacaccgc cgccgagctc ggcgagcagc cgggtccgcg tcagatgggg
     2761 caggtcatga ggaaggcgtc cgctgcgacc gccggtcgtg ccgacggtgg ccgggtggcc
     2821 gctgcggtga aagccctgct cgtctcctga ccgggagccg tgcgcgagca cgagggcggt
     2881 ggcgatcgtc tccgcgtacg gcggcgaccc cgctacttgg taggcgactt cttccgcgcc
     2941 ggtgctcgct tcgccggggt cttcgccggg gtcttcttcg gggcgggacc ccgcgcccgg
     3001 cgctcggcga gcagctccga tgctcgctcg tcggtgagtt gttcgatgct gtcgcccttg
     3061 cgcaaggagg cgttggtctc gccgtcagtg acgtaggggc cgaaccggcc gtccttgatc
     3121 accatcggct tcccggtggc cgcgtcattg cccatctcac gcagcggagg ggtggccgct
     3181 gcggtgcggc gcccgcgctg cttgggctgc ttgtagatct ccagagcctc gtccagggtg
     3241 acggtgaaca gctgctcctc gtcggtgagc gaccgcgagt cggtgccctt cttcaggtaa
     3301 ggcccgtagc gcccgttctg cgcggtgatc tcctcgccgc tctccgggtc ggtgccgacc
     3361 agccgcggca gcgagagcag gcgcagcgcc tcctccagcg tgacgctctc ggtggacatg
     3421 cccttgaaga gcgatccggt gcggggcttg gccttggccg acgccttctt cttcgcgccg
     3481 ttcgtgggcg tctcgggctc cgcgggctcc gggaggatct ccgtgacgta gggcccgtag
     3541 cggccctcct tcgcgacgat ctcgtggccc gtcatcgggt cgacacccag cgagcggccc
     3601 tcctgcggca ccgagaacag ctgctcggcg acctcgggcg tgagctcgtc gggcggaagg
     3661 tcgtcaggaa ggttggcccg ctgcgactgc ccgtcgcgaa tgcgctccag gtacgggccg
     3721 tagcggccga cgcgcaccac gacgtcgtcg aacaccggga cggagttgat ctcccgggcg
     3781 tcgatctcct cgaggttgac gccaacgagc ttcttgaggc ccccggaccg cgccaccgac
     3841 cctgcactgc cctggtcggc cccgaagtag aagcccgaga gccagtcggt gcggccctgg
     3901 gtgccggcgg cgatcgagtc gagctcgtcc tccatcgcgg cggtgaagtc gtagtcgacc
     3961 agccggccga aatggtgctc gagcagtccg accacggcga acgcgatcca cgaggggacg
     4021 agcgcctgac ccttcttcca gacgtacccg cggtcctgga tcgtcttgat gatcgacgcg
     4081 taggtggacg ggcggccgat cccgaggtcc tccagcgtct tgatgaggct gggctcggtg
     4141 aaccgcgacg gcgggttggt cgagtgccct tccggggtca gccgggcagc ggtcagcgcc
     4201 tgcccctgcg tgagctccgg gagccgcgac tcggcgtcgt cggcctcacc acccgcctgg
     4261 tcgtcgacgg tctcgacgta ggccttgagg aacccgggga aggtgatcgt gcgtcccgac
     4321 gctgcgaacg tgaccgcctc gccggtggcc gcggtgccgc cgatgcgcac gctgacggtg
     4381 gtgccccgtg cgtcggccat ctgcgacgcc acagtgcgct gccagatcag ctcgtagagc
     4441 cggaagtcgt cgccgtcgac ctcgcgggcg atctcgcccg gggtgcggaa ggtgtcgcct
     4501 gcgggccgga tcgcctcgtg ggcctcctgc gcgttcttca ccttgcgcgt gtactgccgc
     4561 ggctgcgccg ggacgtaggc gtcgccgtag agctcgcggg cctgcgagcg ggcggcctgg
     4621 atcgccgact ccgacagagt ggtggagtcg gtgcgcagat aggtgatgta gccgttctcg
     4681 tagagccgct gcgcgctgcg catcgtgcgg tcggcggaga agcgcagctt ccggccggct
     4741 tcctgctgca gcgtggacgt catgaacggc gcgtacggct tgcgggtgta gggcctcgac
     4801 tcgaccgact cgacagccag gtcgcggccc tgcagcgcat cggccaaccg gcgggcgctc
     4861 gcctcgtcga gggggcgggc cgtctccttg ccgtcggcgc gattgggact gccgtcagcg
     4921 cccttgagcc tgccgtcggg gccgaagtcg cggcccgtgg ccacccgggt gccgtcgacg
     4981 gccacgaggc gcgcagggaa ctgcctcggg ctcgcgtcgg cgccggcgtc gagctgcgcg
     5041 gcgatgtccc agtacgacgc cgacacgaac gccatgcgct cgcgttcgcg ctgcacgatg
     5101 attcgcgtcg ccaccgactg cacccggccc gccgacagct tcggcatgac cttcttccac
     5161 agcacggggg agacctcgta gccgtagagc cggtcgagga tgcggcgggt ctcctgggcg
     5221 tcgaccaggt cgtggtcgag gtcacgcagg ttctccacgg ccgctcggat ggcggactcg
     5281 gtgatctcgt ggaagaccat ccggcgcaca gggaccgtgg gcttgagcgt ctcgagcagg
     5341 tgccaggcga tcgcctcgcc ctcacggtcg tggtccgtgg cgagcaggag ctcgtcgacg
     5401 gacttcagcg cgtccttgag ctcggcgacc ttgcccttct tctccggcgt gatgatgtag
     5461 agcggcgtga actggttgtc gacgtccaca ccgagtcggg cccaggcctc gcccttgtac
     5521 ttggccggga cgtcggcggc cccgcgcggc aggtcgcgaa tgtggccgac cgacgcctcg
     5581 accacgtagt tgttgccgag gtactgctgg atcttcttcg ccttggtccc ggactcgacg
     5641 atcaccaacc gccgcgtcgg ccgtccgttc gagacgaccg caggcgtgcc ggtaggccgg
     5701 cccgggtcag ccttcgcggc cgccttccgg ctgcccgtgc ccctcggggt cgcgggcttc
     5761 gcactcgtcg ttccggttgc cacctggtcc tactccatcg tctacggccg tatcggcgct
     5821 gcgcaccggc cgtcctccag ggccaccgcg ccggatgcgc ggggtgcccg gacaccgagt
     5881 atgcgtgtcg caagttccgg cacgtaccgg aacctcacgt tcgcgcaccc acccacggtg
     5941 tcacactcat cacaccgcgc gtcacgcagc ggagcgagtc cgacactgtg ccggtctgcg
     6001 ccgcgcgagc ctaaccagcc cgtcaagacg cgttccggcc ccagtaccgc ggccagtccg
     6061 ccgtgtcggc cctgggcggg accggcccga cgagctcgac gagccgttgg acccgtcgcg
     6121 tgccgctgat ccgcagagcc tgtccacgac ccacccgggc gggtggtatt cccgctctgg
     6181 tggctgccgc caccagagga aggtgcgttt cgggcgcgtg cgggtcgagc gccagcaggt
     6241 acccaccccg ctcgtcgggc cggccggcgg ccagtgccca cagtcgtaac gccggtccgt
     6301 ccaattgcca tcccggcggg accgttttga ccgcaccgcg cgtccacgcg cgcgccagcc
     6361 ccaccaggtc gcgccggaac gccgagcgca gcgcggtggt gccgccctcg gtctgcacgg
     6421 cctccatcgt gatcccggcg gccgcgctgg ccgccgcgag cgccggggcc ctggccgggt
     6481 cggcgagcac gatcgacagc cgcgcctggt cgcctgcgcc gaagcggacg atctgtcccg
     6541 gtccgcagag cagcccggcg aggtcgctca ccctgggcgg gcgcgcgtcc gcggagaaca
     6601 gcgacagctg cgacacgacg cgcacagtag aacacccgtt cgaccgaaat cacggggacg
     6661 cgccgaagat cgccacgccg tgtcctggtc aggcgagaaa ggccagcagc tcgcgggcga
     6721 gatcggagga gaaatccgcg aggccgacgg ctaggagcgc gctgaacgct cctagcgcgg
     6781 tgcggcggtc gacaatgccg acagctgctg gcactgcgcc gcacgctccg ccgcgcgctg
     6841 cagccggggc gtggagctca ggtcgcccag cgggttcggg ggtgtgatcg catcgaaggt
     6901 cgactgggcc cgtgtcagcg ccgccttgac ggagtcggga ttgttcgggt cggcagtgtc
     6961 caccgcgttc ttggcccccg tgaagtcctg ctcgaggaac tccaacgcgc tctcggcccg
     7021 gccgacggcc tcggcgccgc cctgcagggg ggagcggccg acggctgcga gttgcgtccg
     7081 gccctgctgc acgccgatga ggacggcacc gaggtagtca ctgacggccc gctggacggt
     7141 gggcaggtcg gccgccgggg cgaggacggg cggcgtcgtg gccggctttg cgaagctcag
     7201 cacggctccg cacacgtggt cggcccagac gacggggtcg ctccccgggc cctcggtggg
     7261 ggtcggggcg ggatctgcgg ctgggctgcc gggcaccgcg gtggtgcagc ctgcgaggac
     7321 ggcgaggacc gcagcaacgg cggggacggc ggcgatccgg acggccgcga tcagggtggc
     7381 ggagatctgg gcggggcggg tcgtgcgagt catgcgccgg ctccgggtcg ggggaaggac
     7441 ggcgtcagtg tgccgggggc gcccggtgct gatcacgcag gccccggccg gcggaccgtc
     7501 aaccgccgac ctggcgctgg atctgctggc agttcggcgc ctgcgtgctg gcccgttgca
     7561 gctccgggtt ggtctgcagg tcgaccgtcg ggtccggcat gttcgcgagc tcctgcagcg
     7621 gcgcaaccgc cttcggcagc tcggtgatga gcgcctgctg atcagtggtg tcgaccgcat
     7681 cgacctgggt cttggcgttc tggaacgagg tccggaactt ggtgagggtc tcggtcaacc
     7741 gagtgaccac ctggtcaccg cccgacaccg gggaggggcc tacccgtgcc attccggtga
     7801 tcgccgagtc ggccgcgccc gcgccctgcg cgaggtagtc gctgatgctc ttgacgagcg
     7861 ctgtggggtc ggcggccgag gtgagctggg gtggggtgcc cgccgcccgg acgaagggca
     7921 gcagctcgcc gcacacgcca tccacccact gcaccgagtc gaccgtggtc gtcgtcccct
     7981 gagtcgaacc ggcaggactc gccgaccccg agacggtcgt cgtgcagccc aggctcaaca
     8041 acagcggcgc gaggatcgtg aggagcagaa cgggacgcgg gacggcgggc atgctgctcc
     8101 caactcgggg ggacgtggtc ggcggccagt gtgcccgccg cggcaagcgg tgcaccgacc
     8161 cgtgcgtttc agcgtttcgg cgaggtcgcc gagcggacct gctggcagtt cgcggccttg
     8221 tcggcggccg ccctgagctc gtcggtggcc cgcaggccgt ccgtggggtc ggccaggttc
     8281 cgcagctcct gcaggggcgc cacggctgcc ggcagcgcga cagacatggc ctgcacgctc
     8341 gatgtgtcca cgccggcgag ctggcccctg gccgtgtcga agctcgtgcg gatctcggtc
     8401 agggcgccct tgagccgggc gacgtactcg tcgccgccct cgaccggtga ggggccgacc
     8461 gcgtccagtt cgctgatcga cccctgcagc gccgtggccg tggcgctgaa gtagtcgccg
     8521 agcccgcgca ccgtggaaac cgggtcggcg ccgccgacct gtggctggga cgtggcggcc
     8581 cgggcgaacc cggacagtgc gccgcacacc tggtccgtcc aggccaccgc gtcggagtcg
     8641 gcgtcacctg tcccgccaca ccctgcggcg atgacgagca cgaacgctga ggcgagagtg
     8701 caggtgaacc tgcgggtacc cattcggtcc tccggcggca gtgcgaccaa caggacgcgc
     8761 ggtgcgcgtc aaccagcggg aaggtactgg tagctccctg caacaacgga ggccgcccac
     8821 cccgagtggg tgaacggcct ccgggcggat gcgagcggtc agccgaccga gctcgctgcc
     8881 ggggtgtcgg cgatcgaggt ggagcgccgc ttcgacacga cgaccgcagc cacgatgatc
     8941 gcgaccgcga ccagcgcgat cgcgatccgg agcggggccg aggccgaggc accgatcgag
     9001 aaggtcacca cggccggtgc gatcagcacc gagaccaggt tcatgacctt gagcagcggg
     9061 ttgatcgagg ggcccgcagt gtccttgaac gggtcaccca cggtgtcgcc gatgatcgtg
     9121 gcctcgtggg cgggagatcc cttgccgccg tggttgccgt cctcgaccag cttcttcgcg
     9181 ttgtcccatg cgccaccgga gttggccagg aagaccgcca tcagcgtgcc gcaggcgatg
     9241 gccccggcaa ggtagccggc cagcgggccg acgccgaggc cgaagccgac ggcgatcggc
     9301 gcgaagatcg ccagcaggcc ggg
//
LOCUS       DASRSC010000087         2701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3629011,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000087 DASRSC010000000
VERSION     DASRSC010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3629011"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..567)
                     /locus_tag="VFE39_04435"
     CDS             complement(211..567)
                     /locus_tag="VFE39_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HET6257880.1"
                     /translation="MVNRQARGRGELESAVLDALFASSAAMTAGEVQQALTGDLAYTT
                     VLTILVRLQDKGALERERRGRAYAYRPVSDEPGLVARRMRRALDERPDRDAVLARFVD
                     DLSDDDEQTLRRLLGE"
     gene            complement(634..858)
                     /locus_tag="VFE39_04440"
     CDS             complement(634..858)
                     /locus_tag="VFE39_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257881.1"
                     /translation="MGEPSRKGRSSALLIGCLGLMLASLVLGLVLILTVTIPLRDTDP
                     VTAGISLILAAFSFVLAGAGVALAALSRRR"
     gene            complement(889..1419)
                     /locus_tag="VFE39_04445"
     CDS             complement(889..1419)
                     /locus_tag="VFE39_04445"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HET6257882.1"
                     /translation="MRGLALSVTMLLAVMALAACGSSAPPGAQATAAAPSTTEVPPQL
                     AVPAGQRAVLTATVERGSQIYACHAGAWVLKQPAAALTAPTTAVLHDAGPRWTATADG
                     SSVTGAQVASVPVPGSAADLLVRATGHDGQGVMATVDFVQRLGVQGGAPPAGACTEGA
                     QQAVGYTAEYRFYAPG"
     gene            complement(1560..2162)
                     /locus_tag="VFE39_04450"
     CDS             complement(1560..2162)
                     /locus_tag="VFE39_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HET6257883.1"
                     /translation="MPRQLDLTDVLAQETAAERERLADLYAGLGPDQWSAQSLCAGWR
                     MREVLAHVTLPYRHSTFRVLWGIAVARGDFNRFADRIARTDTADLSDAELLAALRENV
                     EHPWRPPGGGQGGALSHDVIHGLDVTEPLGLPRPPAQRVRHVLAAAGPRALAHFGTDL
                     TGVRLVATDTDIALGEGPDIVELPAVDVLLRVTGRTRIAA"
     gene            complement(2220..>2701)
                     /locus_tag="VFE39_04455"
     CDS             complement(2220..>2701)
                     /locus_tag="VFE39_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HET6257884.1"
                     /translation="TAAANSALQRARETLDRHRPAPGDPLDAGDAIQRDLLERYVTAF
                     EAHDVARLADLLCEDARTSMPPFARWLEGGTTIATLIGAGGCEGARLVATAINGQPGF
                     GQYRPDDDGRLHPFALVLVEPRGQRIAHTTTFLGTAERFPAFDLPVVLEPTPSTLDR"
BASE COUNT          399 a          931 c         1005 g          366 t
ORIGIN      
        1 cgtctcgtcg tccgcaacgg gttcacccac agcctcgccg agcaactgat ggacgacctg
       61 cgccgtgtga tcgctcgcct ggatcgtcag caacagccga tgcgcgacgc ccgcaccgac
      121 agctccttca gccataccgg agcccccagc ccgcagtgat cacgggctac ccgccggatg
      181 cgggtgcagc ggagaccgcg atacgcatcg tcactccccg agcaggcgcc gcagcgtctg
      241 ctcgtcgtcg tcggagaggt cgtcgacgaa acgggcgagc accgcgtccc gatcagggcg
      301 ctcatcgaga gcgcggcgca tccgtcgggc caccagaccg ggctcgtcgc tcaccgggcg
      361 gtacgcgtag gcgcgtcccc ggcgttcccg ttccagcgcc cccttgtcct ggaggcggac
      421 gaggatcgtc aacaccgtgg tgtaggcgag atcgccggtg agggcctgct ggacctcacc
      481 agcggtcatg gccgcggagg acgcgaacag cgcgtcgagc acggcggact cgagttcccc
      541 cctgcctcga gcctgccggt taaccacggt tcccctccct cgacgacgac agaactgtag
      601 tagcgggtgc gcactcatgt cattcggcgc gagctaccgc cgacgactga gcgcagcgag
      661 cgcgactccc gcgccggcga gcacgaagga gaacgccgcg agtatgagag agatcccggc
      721 ggtgacggga tcggtgtcgc ggagcggaat cgtcaccgtc agaatcagaa cgaggccgag
      781 gacgaggctg gcgagcatca acccgaggca accgatcagg agtgccgacg agcggccctt
      841 ccggctcggc tcacccacgg gagtgcagcc gaggaagccc accccgagct agccgggggc
      901 gtagaagcgg tactccgcgg tgtaaccgac ggcctgctgc gcgccctcgg tgcacgcgcc
      961 cgccggaggc gcgccaccct ggacgccgag gcgctgcacg aagtcgacgg tcgccatcac
     1021 cccctggccg tcgtgtccgg tggctcgcac tagcagatcg gcagcggatc cggggacggg
     1081 caccgacgcc acctgtgctc ccgttaccga gctgccgtcc gccgttgcgg tccaccgggg
     1141 tccggcgtcg tgcagcacgg cggtcgtggg agcggtcagc gccgcggccg gctgcttgag
     1201 cacccaggcg cccgcgtggc acgcgtagat ctgtgagccg cgctccaccg tcgcggtgag
     1261 taccgcgcgt tggccggccg gcacggcgag ctgtggcggc acctccgtgg tgctcggagc
     1321 ggctgcggtg gcttgagcac cgggaggggc tgacgatccg cacgccgcga gcgccatcac
     1381 cgcgagcaac atcgtcacgg acagggccag gccgcgcacc cgtcgacacg gatggagctc
     1441 gcacgcatca cccccgcgga ggccgtcgaa gagctgtcgg gcgcggtctt cctccatcga
     1501 gctgaagcac tcccctcacc ggatctccag ctttactacg tcatcgtagt acgggctggc
     1561 tacgcggcga ttcgcgtcct cccggtgacg cgcagcagga catccaccgc gggcagttcg
     1621 acgatgtcgg gcccctcgcc cagggcgatg tcggtgtcgg tcgcgaccag tcgcacgccg
     1681 gtgagatctg tgccgaagtg ggcgagagcg cggggacccg cggcggccag gacgtgccgt
     1741 acccgctggg cgggcggccg aggcagcccg agcggctcgg tgacgtcgag gccgtggatg
     1801 acgtcgtggc tcagcgcgcc gccctggccg ccgcccgggg gtcgccacgg gtgctcgacg
     1861 ttctcgcgta gcgcggcgag cagctcggcg tcagacaggt cggcggtgtc ggtgcgggcg
     1921 atgcggtcgg cgaagcggtt gaagtcgccg cgggcgaccg cgatgcccca cagcacgcgg
     1981 aaggtcgagt ggcggtaggg caaggtgacg tgggcgagga cctcacgcat gcgccacccg
     2041 gcgcagaggg actgcgccga ccactgatcg gggccgagcc cggcgtagag atcggcgagc
     2101 cgctcccgtt cggcggcggt ctcctgggcg agtacgtcgg tgaggtcgag ctgacggggc
     2161 acggggtctc cctcgcggtg gggtgctgac ccttccacta cgtcaggcgg cccccggaat
     2221 catcggtcga gcgttgacgg cgtgggttcg agcaccaccg gcaggtcgaa cgcgggaaag
     2281 cgctctgccg tgccgaggaa ggtcgtcgtg tgcgcgatgc gctgtccgcg gggctcgacc
     2341 aggacgagcg cgaaggggtg cagtcgcccg tcgtcgtcgg ggcggtactg gccgaagccc
     2401 ggctgcccgt tgatcgcggt cgccacgagc cgggcgccct cgcagccgcc cgcgccgatg
     2461 agggtcgcga tcgtcgtgcc tccctcgagc caccgggcga agggcggcat cgacgtccgg
     2521 gcgtcctcgc acaaaaggtc agcaagacgg gccacgtcgt gggcctcgaa ggcggtgacg
     2581 taccgctcga gcaggtcccg ctggatggcg tcgcccgcgt cgagggggtc gcctggtgcg
     2641 ggacggtggc ggtcgagggt ctcgcgggcg cgctggagcg cgctgttggc ggcggccgtc
     2701 g
//
LOCUS       DASRSC010000088         7013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5432528,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000088 DASRSC010000000
VERSION     DASRSC010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7013
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5432528"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="VFE39_04460"
     CDS             <1..592
                     /locus_tag="VFE39_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HET6257885.1"
                     /translation="HAMLELEPTGPVVDPFDVPGGWRIGEPAWTTWQMLVAGHEPVIV
                     QVSGRASSAECVVGDRTPVSVRAHRRPDALEVTVDGLTRRYHWAHDGGTLWLGRDGHA
                     WGVREQAPLQAATAEAGAGGPVLSPMPGTVTVVEVAEGQQVAAGDRLVVVEAMKMEHV
                     LTAPVDGVVRELRAHPGATVARDAALLTVEPITEEG"
     gene            594..1745
                     /locus_tag="VFE39_04465"
     CDS             594..1745
                     /locus_tag="VFE39_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6257886.1"
                     /translation="MDLTLSDEHETLRATVEDFARKEVAPVIGEFYERGEFPYDIVAK
                     MGAMGLFGLPVDESYGGMGGDYFALCLALEELARVDSSVAITLEAGVSLGAMPIYRFG
                     TEEQKREWLPALTAGERLGAFGLTEPGGGSDAGATRTTATLDGDEWVINGSKAFITNS
                     GTDITSVVTVTAITGGRDVAGRREISAIMVPAGTPGFRVSKKYSKVGWSASDTRELFF
                     DDCRVPAANLLGERGRGYAQFLSILDEGRVAIAALSVGLAQGCVDESVRYAHEREAFG
                     NPIGSYQAIQFKIADMEARTHTARLAYYAAAAKMLAGEPFKKEAAIAKLVASNAAMDN
                     ARDATQVFGGYGFMNEYPVGRFYRDAKILEIGEGTSEVQRMLIARQLGL"
     gene            1797..3248
                     /locus_tag="VFE39_04470"
     CDS             1797..3248
                     /locus_tag="VFE39_04470"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET6257887.1"
                     /translation="MTALFARIVVIVSGAAILVVETLSTRLVAPYVGLTLESSTAVIG
                     VALAGIAAGASLGGRWADVLPPRRVAAGALAVGGLGVLVVRPLIRLLGPVLGPGPYAA
                     LLLVAVSTLVSVTALAMVTPAVTRACLSSISGSGAVVGQLSAAGTLGSLVGVFLTGFL
                     LVSLLPVTAILLLTAAVCLLMALLTATVRTRAGVTGVAVVAVVAGAALVAVPGRCDAD
                     TVYYCARIETDAARPDVRVLVLDDLRHSAVDLSEPARLEFAYTQRFADAIDTAFAAGR
                     ALDAVHIGGGGFTMPRWLAATRPGSTSTVLEVDEGVVELGRRELGVDAIPGTAVRIGD
                     ARTLLAGLPAASADVVVGDAFGARSVPWHLATTEFVADVNRVLRNDGVFVLNVIDRDP
                     WRLLAAELATVATRFPHVALMTSGPDQLGRGGGGNVVIVASDRPLDIAALADRAAVRG
                     EPDSVIGEEDSRRFADGAAVLTDGWAPVDQLIG"
     gene            3287..3676
                     /locus_tag="VFE39_04475"
     CDS             3287..3676
                     /locus_tag="VFE39_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257888.1"
                     /translation="MASWDDLVSFVRVRYEIMRQVESELWFNLPTSGDRTQIVAVRLV
                     TGEDGHPWAQITSPIGLAADIHLGTLLALAGESIVGGAIATDGLVLFRHSIPLGDTAL
                     DGFDRSFRVVVEVADRLEHELTGADEH"
     gene            3915..5459
                     /locus_tag="VFE39_04480"
     CDS             3915..5459
                     /locus_tag="VFE39_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET6257889.1"
                     /translation="MNGARSLIRTLVEAGVEVCFANPGTSEMHFVSALDEIAGMRGVL
                     GLFEGVVTGAADGYARMTDRPAATLLHCGPGLSNGLANLHNASRAGVPIVNVVGDHAI
                     YHKRLNAPLETDIDALARTVSGWVRRSYRPQDVGADAADAVAAAQGPPGQIATLILPA
                     NVSWEDGGVPGSVRTRRPAARVPDAQVAAAATALTSGEPAVLMLGGMAQRERGLRAAS
                     RIAVATGAQVSTQTHPARLQRGVGLPAFDRLPYFVEDAVAQLTGNRHMILAGTAAPVA
                     FFAYPDLPGSPTPQDCAVHELGAPGQDIVEALEHLADRVAPDTAPLLQAPTAPGPRAG
                     ALTTGAIADLVAAHLPEDAIVVDEAVTAGVALAAASVGAPPHDWLCVTGGAIGYGIPT
                     AVGAAVACPGRPVINLQADGSALYTIQALWTQARENLDVTTVLLNNHSYAILEVEMNR
                     VLARSPGDKSRDLLSLTDPDIDFVKIANGLGVAATRATTAEELAGALRFAAAEPGPHL
                     IEAAYS"
     gene            complement(5476..6276)
                     /locus_tag="VFE39_04485"
     CDS             complement(5476..6276)
                     /locus_tag="VFE39_04485"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0106411 - XMP 5'-nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HET6257890.1"
                     /translation="MASSRRLRALITNDDGIDSPGLWALAAGARDAGFDVVVAAPHVD
                     ASGVGGSVLSIRDGNRTRLHRRELADLPDVPAYAVEGHPAFIVHSAGRGWLDPKPDVV
                     LSGINLGANLGHAVLHSGTVGAVLAGSLHGWRGLAVSLDIPEQRPERPHWETVLHVLP
                     EVLDVLLAAPDGTALSLNVPDLPIPEIGELREARLATFGAVQVRVDHSSGEHGEALHS
                     QVAELSAPLEPGTDAALLAAGHPTLTELQSVSERPGVLRRVTTSRRAG"
     gene            6352..>7013
                     /locus_tag="VFE39_04490"
     CDS             6352..>7013
                     /locus_tag="VFE39_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET6257891.1"
                     /translation="MVTRIPMPPTGEQFEVASGDVRAVVTEVGAGLRSFEAGGRPYVE
                     TFDETVRPPRGSGTVLVPWPNRTARGRWTWNGEPQQLTLSEPAAGNAIHGLLRHTLYR
                     PGERTADAITLHAVVAPQPGWPVPLDTSVRFAVGDTGLTVTHTVRNVGGEPVAFGIGA
                     HPYLRAGGDATDDCVLTLAATSTLPLDGGLPTGPAVPVDSDLDFRDGHPLRGRELDHA
                     FGG"
BASE COUNT          947 a         2495 c         2529 g         1042 t
ORIGIN      
        1 ccacgccatg ctggagctcg aacccaccgg tccggtggtc gacccgttcg acgtgcccgg
       61 cggctggcgc atcggtgagc ccgcgtggac gacatggcag atgctcgtgg cggggcacga
      121 gcccgtgatc gtgcaggtga gcggacgggc ctcgtcggcc gagtgtgtcg tcggcgaccg
      181 cactccggtg tccgtccgtg cgcaccgtcg ccccgacgcc ctcgaggtga ccgtggacgg
      241 cctgactcgc cgctaccact gggcgcacga cggcgggacc ctgtggctcg ggcgcgacgg
      301 gcacgcctgg ggcgtccgcg agcaggcgcc gctacaggcc gccaccgcgg aggcgggcgc
      361 aggcggaccc gtgctctccc cgatgcccgg caccgtcacc gtcgtcgagg tcgccgaggg
      421 gcagcaggtc gccgcgggcg accgcctcgt cgtcgtcgag gcgatgaaga tggagcacgt
      481 gctcaccgct cccgtcgacg gggtggttcg cgagctccgc gcccacccgg gcgccaccgt
      541 cgcgcgcgac gccgcactcc tgaccgtcga acccatcacg gaagagggat gacatggacc
      601 tcacgctcag cgacgagcat gaaacgctgc gggcgaccgt cgaggacttc gcacgcaagg
      661 aggtcgcgcc agtcatcggc gagttctacg agcggggcga gttcccctac gacatcgtgg
      721 cgaagatggg cgcgatgggc ctgttcggcc tgcctgtcga cgagtcctac ggcggcatgg
      781 gtggcgacta cttcgccctg tgcctcgcat tggaagagct ggcgcgcgtc gactcgtcgg
      841 tggcgatcac gctcgaggcg ggggtgtcgc tgggtgcgat gccgatctac cggttcggca
      901 cggaggagca gaagagggag tggctccccg ccctgaccgc tggggagaga ctcggcgcgt
      961 tcgggctgac cgagccgggc ggcggctcgg acgccggcgc cacccgcacc accgcgacgc
     1021 tcgacggcga cgagtgggtg atcaacggct ccaaggcgtt catcaccaac tctggcaccg
     1081 acatcacctc tgtcgtcacc gtcaccgcga tcaccggcgg ccgggacgtc gccggtcgca
     1141 gggagatctc ggcgatcatg gtgccggccg ggacgccggg gttccgggtg tcgaagaagt
     1201 actcgaaggt cggctggtcg gcctccgaca cccgcgagct cttcttcgac gactgccgcg
     1261 tgcccgccgc caacctcctc ggtgaacgcg ggcgcggcta cgcgcagttc ctctcgatcc
     1321 tcgacgaggg ccgcgtggcg atcgccgccc tgtcggtcgg gctcgcgcag ggctgcgtcg
     1381 acgagtcggt gcgctacgcc cacgagcgcg aggcgttcgg taacccgatc ggcagctacc
     1441 aggccatcca gttcaagatc gccgacatgg aggcgcggac ccacaccgcg cggctggcgt
     1501 actacgcggc agcggcgaag atgctggcag gcgagccgtt caagaaggag gcggcgatcg
     1561 cgaagctggt ggcctcgaac gcggcgatgg acaacgcccg tgacgccacg caggtcttcg
     1621 gcggctacgg tttcatgaac gagtacccgg tcggccgctt ctatcgggac gccaagatcc
     1681 tggagatcgg cgagggcacc tccgaggttc agcggatgct gatcgcccgc cagctgggcc
     1741 tgtgacgccc caccccgctg aacaccggga gacgggccat caccgaggat gggggcgtga
     1801 ccgccctgtt cgcccggata gtcgtgattg tgagcggcgc cgccatcctg gtggtggaga
     1861 ccctctccac gcggctggtg gcgccgtacg tcggtctcac cctcgagtcg tcgacggcgg
     1921 tgatcggtgt cgcgctggcc gggatcgcgg cgggcgcctc gctcggcggg cgctgggccg
     1981 acgtgctgcc gccgcgtcgg gtggccgcgg gagccctcgc cgtcggcggg ctgggagtgc
     2041 tggtggtgcg ccccctgatc cggctgctgg ggccggttct cgggcccggc ccgtacgcgg
     2101 cgctgctgct ggtcgcggtg tcgacgctgg tgtcggtgac cgcgctggcg atggtgacgc
     2161 ccgccgtcac gcgggcgtgc ctctcgagca tttccggctc cggtgcggtg gtcgggcagc
     2221 tgtccgcggc cggaacgctc gggtcgctgg tgggggtgtt cctcaccggg ttcctgctgg
     2281 tgtcgttgct gccggtcacc gcgatcctgc tgctcacggc cgctgtgtgc ctgctgatgg
     2341 cgctgctgac ggccaccgtg cgcacgcggg cgggtgtcac gggtgtcgcc gtcgtcgcgg
     2401 tggtggccgg cgccgcgctg gtggcggtgc cgggacgctg tgacgccgac acggtctact
     2461 actgcgcccg catcgagacc gacgccgccc ggcccgacgt gcgtgtcctc gtactcgacg
     2521 atctgcggca ctcggcggtc gacctctccg agccggcgcg cctggagttc gcgtacacac
     2581 agcgcttcgc cgatgcgatc gacaccgcct tcgcggcagg ccgcgcgttg gacgccgtgc
     2641 acatcggtgg aggcggattc accatgccgc gctggctggc cgcgacccgg ccgggcagca
     2701 cgtcgaccgt gctcgaggtg gacgagggcg tggtcgagtt gggccgccgt gagctcgggg
     2761 tggacgcgat ccccggcaca gccgtgcgca tcggggacgc caggacgctc ctcgccgggc
     2821 tgccggccgc gtcggcggac gtggtggtgg gcgacgcgtt cggcgcacgg tccgtgccgt
     2881 ggcacctcgc cacgaccgag ttcgtcgctg acgtgaaccg ggtgctgcgc aacgacggtg
     2941 tcttcgtgct gaacgtgatc gaccgggatc cgtggcggtt gctcgcggcg gagctggcca
     3001 ctgtggccac ccggttcccg catgtagcgc tgatgacctc cggcccggac caactcggca
     3061 ggggtggcgg gggcaacgtg gtgatcgtcg cgtccgaccg cccgctcgac atcgccgcgc
     3121 tcgccgaccg cgcggcggtg cgcggggagc cagactccgt gatcggtgaa gaggactccc
     3181 gcaggttcgc cgacggggcg gcggtgctca ccgacggatg ggcaccggtc gaccagctca
     3241 tcggatgatg gcggtacggt ccgttggact gaggagaggt ggcgttgtgg ccagctggga
     3301 cgatcttgtg tccttcgtcc gcgtgcgcta cgagatcatg cggcaggtcg agagcgagct
     3361 gtggttcaac ctgcccacct cgggcgatcg cacgcagatc gtcgcggtgc gcctggtcac
     3421 cggggaggac gggcacccgt gggcgcagat cacctcaccg atcggtctcg ccgcggacat
     3481 ccacctcggc acgctgctcg cactcgcggg tgagtcgatc gtgggcggcg ccatcgccac
     3541 cgacgggctg gtgttgttcc gccactccat cccgctcggg gacaccgcgc tggacgggtt
     3601 cgaccggtcg ttccgcgtgg tggtcgaggt ggccgaccgg ctcgagcacg agctcaccgg
     3661 tgccgacgag cattgaccac ccgaacggca gcagaatgca gatgctgcgg aatgggaccg
     3721 gttacgcgag tgcgagcgaa ttttgcccta cgcgtggcct ggctcagcgc gcgtcggatg
     3781 tggatgtgat cttgcggagg cagcccagga tctgacgggt gggtacggag ccgcagtgcg
     3841 cgcgtctggt actggtatcg ggatggacaa ccgccgtcct cgtcactagg gagaccgctg
     3901 tggaacatct ggcgatgaac ggtgctcggt cgctgatccg gacgctggtc gaagccggtg
     3961 tcgaggtgtg tttcgcgaac ccgggcacat ccgagatgca tttcgtctcc gcattggacg
     4021 agatcgcggg catgcgcggc gtgctggggc tgttcgaggg tgtcgtcacc ggagcagccg
     4081 acggatatgc acgcatgacg gacaggccgg cggccacctt gctgcattgc gggccggggc
     4141 tgtccaacgg gttggccaac ctgcacaacg cgagccgggc gggtgtgccg atcgtgaacg
     4201 tggtgggcga ccacgccatc tatcacaaac ggctcaacgc gccgctcgag accgacatcg
     4261 acgcattggc ccgcactgtt tccgggtggg ttcgtcgctc ctaccgtccg caggacgtgg
     4321 gcgccgacgc cgctgatgca gtagccgcgg cccagggtcc gcccggacag atcgccacgt
     4381 tgatccttcc ggccaacgtc agctgggagg acggcggcgt cccgggatcg gtccgcaccc
     4441 gtcgcccggc cgcacgtgtg cccgacgcac aggtggcggc cgccgcgacc gcgctgacga
     4501 gcggcgagcc tgccgtgctc atgctgggcg gcatggcgca gcgcgaacgc ggcctgcgtg
     4561 ctgccagccg gatcgccgtg gccaccggag cgcaggtatc gacccagacc catcccgctc
     4621 gcctccaacg cggcgtcggc ctgcccgcct tcgatcgcct tccctacttc gtcgaggacg
     4681 ccgtcgcaca gctcaccggc aaccggcaca tgatcctggc cggcaccgcc gcgcccgtgg
     4741 cgttcttcgc ctaccccgac ctccccggct caccgacgcc gcaggactgc gccgtccatg
     4801 agctcggcgc gcccggccag gacatcgtcg aggccctcga acacctcgcc gaccgtgtcg
     4861 ctcccgacac cgctccgctg ctgcaggccc ccaccgcgcc tggcccgcgc gccggtgcac
     4921 tcaccaccgg tgccattgcg gacctggtcg cggcgcacct gcccgaggac gccatcgtgg
     4981 tcgacgaagc ggtcacggcc ggcgtggcac tggccgccgc gagtgtcggc gcacccccgc
     5041 acgactggct ctgcgtcacc ggcggcgcga tcggctacgg catccccacg gccgtcggcg
     5101 ccgccgtggc ctgccccgga aggccggtga tcaacctgca ggccgacggc agtgcgctct
     5161 acaccatcca ggccctctgg acgcaggctc gagagaacct ggatgtcacc acggtgctgc
     5221 tcaacaacca ctcgtacgcg atcctcgagg tcgagatgaa ccgcgtcctg gcccgctctc
     5281 ccggtgacaa gtcgcgcgac ctgctctcac tcaccgatcc cgacatcgac ttcgtcaaga
     5341 tcgcgaacgg cctcggtgtc gccgccaccc gcgccaccac cgcagaagag ctcgccggcg
     5401 ccctccggtt cgccgcagcc gagcccgggc cgcacctcat cgaggccgcc tattcgtgag
     5461 ctcagctgag cgggatcatc ccgcccgcct gctcgtcgtc acgcggcgca gtacacccgg
     5521 tcgctcggag accgactgca gctcggtgag cgtcgggtgg cctgcggcga ggagcgcggc
     5581 gtccgttccg ggttccagcg gggccgacag ctcagcgacc tgggagtgca gtgcctcccc
     5641 gtgctcccca ctgctgtggt ccacccgaac ctgcaccgcg ccgaaggtgg cgagccgggc
     5701 ttcgcgcagc tcgcctatct cggggatggg caggtcgggc acgttcagcg acagtgcggt
     5761 gccgtccggg gcggcgagca gcacgtcgag cacctcgggc aggacgtgca gcaccgtctc
     5821 ccagtgcggg cgttccggcc gctgctccgg gatgtccagc gagacggcca gcccccgcca
     5881 cccgtgcagc gacccggcca gcaccgcgcc caccgtgccc gagtgcagca ccgcgtgacc
     5941 caggttggcc ccgaggttga tcccggacag caccacgtcc ggcttcgggt ccagccatcc
     6001 gcggccggcc gagtgcacga tgaacgccgg gtggccctcc acggcgtagg ccgggacgtc
     6061 gggcaggtcg gcgagctccc ggcggtgcag gcgcgtgcgg ttgccgtccc ggatcgacag
     6121 caccgagccg ccgacaccgc tggcgtcgac gtgcggcgcg gccacgacca cgtcgaaccc
     6181 ggcgtcgcgc gcaccggctg cgagggccca cagtcctggt gagtcgatgc cgtcgtcgtt
     6241 ggtgatcagg gcgcggagac gtcgggagga ggccacccgt ccaccgtatg cgaggtaggt
     6301 ggccattggg cgacacggcg atgaccctca ccgccgcact aggctcccgc ggtggtcacg
     6361 cgcatcccca tgccgcctac gggcgagcag ttcgaggtcg cctccggcga cgtccgcgcc
     6421 gtcgtcaccg aggtcggtgc gggcctgcgg tccttcgagg cgggcggacg cccgtatgtc
     6481 gagacgttcg acgagacggt gcgcccgccg cgcggctcgg gcaccgtcct cgtcccctgg
     6541 ccgaaccgca ccgcccgggg ccgctggacg tggaacggtg agccgcagca gctcaccctg
     6601 agcgagcccg ccgcaggcaa cgccatccac gggctgctcc ggcacaccct ctaccggccc
     6661 ggggagcgga cggccgacgc gatcacgctg cacgccgtgg tcgccccgca gccgggctgg
     6721 ccggtgcctc tcgacaccag cgtgcggttc gccgtgggtg acacggggct caccgtcacc
     6781 cataccgtgc gcaacgtggg gggcgagccg gtggcgttcg gaatcggtgc gcacccgtac
     6841 ctgcgggcgg gcggcgacgc cacggacgac tgcgtgctca cgctcgccgc cacctcgacg
     6901 ctgccgctcg acggcggcct acccacaggc ccggccgtgc ccgtcgacag tgatctcgac
     6961 ttccgcgacg gtcacccgct gcgtggtcga gagctcgacc acgccttcgg ggg
//
LOCUS       DASRSC010000089        10645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1502514,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000089 DASRSC010000000
VERSION     DASRSC010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1502514"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1343)
                     /locus_tag="VFE39_04495"
     CDS             complement(<1..1343)
                     /locus_tag="VFE39_04495"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET6257892.1"
                     /translation="MRVSQSWLAELLDDVPPADETAEAFVRVGLEIEDVHRAPELTGP
                     LVVGRVLEFEVLTGLKKPIRWCQVDVGESQSRGIICGAGNFEAGDLVVVALPGAVLPG
                     GFAISARKTYGHVSDGMIASARELGIGADHAGILVLPPATAEPGDDARPLLGLAPDDP
                     DTVIELSVTPDRGYCFSVRGLARVLAASLDADYTDPAARVAVPAAYGDAWPVELADRG
                     CARFVARRVDGVDPTAPAPWWMQRRLLAAGMRPISLIVDVTNYVMLELGQPLHAYDAA
                     RVSGPIVVRRAEAGEKLRTLDDVERTLDPDDLLITDDSGPIGLAGVMGGASTEISGPG
                     ESGSIDVLIEAAHFDPAVIARAARRHKLPSEASRRFERVVDPQLPPVAAERAAQLLVK
                     HGGGTIAPGRTDAGATPRPVPVEMPLELPDRVAGIAYPPGATVRRLAQVGCAVELV"
     gene            complement(1376..2443)
                     /gene="pheS"
                     /locus_tag="VFE39_04500"
     CDS             complement(1376..2443)
                     /gene="pheS"
                     /locus_tag="VFE39_04500"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET6257893.1"
                     /translation="MTESTDVMSLLHPDSLDAAVAAAVQAFAGASDLAALAAVKPAHL
                     GDRAPLLLARRELGSLPGPDRAEAGKRVNAARQRAQEAFDARREVLVAERDERVLVEE
                     TVDVTLPWDRIPRGARHPLTQLSERIADVFVGMGWEVAEGPEVESSWLNFDALNFGKD
                     HPARTMQDTFYLAGSTAGEDSEDSGTVLRTHTSPVQIRALLERDLPLYVVCPGRTFRT
                     DALDATHTPVFHQVEGLAVAKGLTMAHLKGTLDAFARSMFGTESKIRMRPSFFPFTEP
                     SAEPDVWFPEKKGGAGWVEWGGCGMVHPNVLRACGVDPDVYSGFAFGMGLERTLMFRN
                     GIPDMRDMVEGDVRFSRAFGI"
     gene            complement(2436..3350)
                     /locus_tag="VFE39_04505"
     CDS             complement(2436..3350)
                     /locus_tag="VFE39_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET6257894.1"
                     /translation="MVTEPPGTPIGASAEIALTQRTPRVVAARKLLRRAGRDRAGRFL
                     VEGAQAVREAVEWAGPRSGRVHELFVTDGAAARHPELVSAAADIGARVSRVTDRAAES
                     LSDTVTPQGLVAVCDLLDVPLPTALTNRPGLVAVCAGIADPGNAGTVIRVADAAGADA
                     VLFAGETVDPHNGKAVRASTGSVFHLPLARHRDATAVLDACRAVGLTLLAADAHGEFD
                     LHDPAADDVLAGPVAWIFGGEAHGVPATVADQAEHRVRVPIHGRAESLNLATAAAVCL
                     YASVAAQRRRATVGESPPASTLGSSHHD"
     gene            complement(3368..3757)
                     /gene="rplT"
                     /locus_tag="VFE39_04510"
     CDS             complement(3368..3757)
                     /gene="rplT"
                     /locus_tag="VFE39_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET6257895.1"
                     /translation="MARVKRAVNAQKKRRTTLELASGYRGQRSRLYRKAKEQMLHSLN
                     YAYRDRRAKKGDFRQLWITRINAAARANGMTYNRFIQGLKASGVEVDRKILSELAISD
                     PAAFTALVEVARANVPAQTGAQDGTAA"
     gene            complement(3814..4008)
                     /gene="rpmI"
                     /locus_tag="VFE39_04515"
     CDS             complement(3814..4008)
                     /gene="rpmI"
                     /locus_tag="VFE39_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET6257896.1"
                     /translation="MPKNKTHSGMSKRVKVTGSGKLMRQQAGLRHRLEVKSSKETRDL
                     SRVVPVNKADVKRVKKMLGR"
     gene            complement(4085..4660)
                     /gene="infC"
                     /locus_tag="VFE39_04520"
     CDS             complement(4085..4660)
                     /gene="infC"
                     /locus_tag="VFE39_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET6257897.1"
                     /translation="MTSIRGDPISTETRINDRIRVPEVRLVGPNGEQVGIVRIEDALR
                     LAQEAELDLVEVAAQARPPVCKLMDYGKFKYESAQKARESRRNQQLTTIKEQKLRPKI
                     DTHDYETKKRNVVRFLEGGNKVKVTIMFRGREQSRPELGFRLLQRLAEDVAELGTVEA
                     APKQDGRNMTMVIAPNKKPAPRPRPATPADA"
     gene            complement(4899..7874)
                     /locus_tag="VFE39_04525"
     CDS             complement(4899..7874)
                     /locus_tag="VFE39_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET6257898.1"
                     /translation="MAGTEGTGAVVGVLGPLVLQDDGGATVRLGSGRQRRLLAALALH
                     AGADVACDTLAELVWGDELPADPDGALQTNVARLRRLLPAPAAIGTGPRTYRLDVSPE
                     GVDAARFAAHLARAAQEPGPARRLAELDAALSLWRGRPFAELDHPDVEPEVARLAGLR
                     ATAVEQRAEALLAAGRTGEAVAAAEALVAAEPLREGAVAILVRGLAAAGRPGDALRAY
                     GRLRGELAEQLGLDPSPELRALHEQVLRHEAPGRAPPTAHARRPAATVVPISSFVGRD
                     GDLARAVAVLAKRRVVTLRGPGGVGKTRLARHVAAAVAGRYADGAAMVELASARPDAV
                     VSAVAGALRLSDAGPGSLTDRVVEVLAVRRQLLVLDDCEHVADAVAALVEAVVIGAPG
                     VDVLATSREALRVDGEQVLPVAPLAPGPAAVLLADRISAAGLAATPTSVDAALVQRIC
                     ARLDGLPLALELAAARVPAVGLAGLLDALEEPLDVLRHGRRTAALRHRSLRDVVAWSF
                     GLLDDEQRRLFVRLGVFSGAVEHAAVADVCGTTAALPDLVDRSLVVRQEGRDGRPTAY
                     RILDTLRAFAREQLASDPAAAPLRARHARWAVDLAVDVGAARSRPDEPAAVRRFDAHL
                     ADVRRAHAWLCTEGPLEDVLRLGLVCAELGFQRARVDLVQMADAALRAAGCEPDRGGP
                     DRDDPDHRQPDGAASLHPLLPRLLGLSAAPRWQRGDVEGAERRCRRALAFVERLGDPL
                     LARDALEVLANILMFRGDLAHAVEYARGAAELAEAAGDEATLVMALTDLVIITAYAGD
                     DGAAARHEAAAGLLAERMGSSLARGWAAYAAGERRAEAGEPGAAAFLERAVELAEEVD
                     AAFLAGVARHTLLTTAARAGDPAQAPARFGPLLDIWHGMGAWTQLWVAVRALAEALSR
                     YGRHRDAVMLLGAVRASPRASAEYGADSARIRAVEDAARVALGAEYDGLHAAGAAMGD
                     SGALALARRLARGDGSTTP"
     gene            8554..8730
                     /locus_tag="VFE39_04530"
     CDS             8554..8730
                     /locus_tag="VFE39_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF397 domain-containing protein"
                     /protein_id="HET6257899.1"
                     /translation="MKSSLSGAAGNCVEVAALDSGEFAVRNSRHPTGPALVFTRAEWL
                     AFLGGVEHGEFRPA"
     gene            complement(8754..9647)
                     /locus_tag="VFE39_04535"
     CDS             complement(8754..9647)
                     /locus_tag="VFE39_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6257900.1"
                     /translation="MAAGGRSEPTSADGESGRGATARRIVLGAQLRRLREAAHISRAD
                     AGYAIRSSDSKVSRLELGRVAFKERDVSDLLTMYGVTDPGERDAFLEMIKQANEPGWW
                     RRYSDVMPTWFHDFVGLEESASRIQTYELQFVPGLLQTEAYARSIATRGRPEYASDDA
                     ERRVTLRMRRQKILTGMNSPKLWVVLDESVLHRPIGGRAVLRAQLEQLLELSKLSNVT
                     IQIVPYHLSGYAAEGSFTVLRFAEPELPDVVYVEHLSGALYLDRQDEVELYSRAVDRL
                     SVDADTPERSRQILARALAEI"
     gene            complement(9718..10530)
                     /locus_tag="VFE39_04540"
     CDS             complement(9718..10530)
                     /locus_tag="VFE39_04540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET6257901.1"
                     /translation="MAPSAAIDRTRASVARVYDCFLGGKDNYDVDREVFEQVNRAAPQ
                     MSRLARDHREWLVRVTRFLTRSAGIDQFLDCGAGLPTAENTHQVAQRMNPEARVVYVD
                     NDPVVAAHGRALLEENDQTHLVMADLTEPDQVLEHPVVTKFLDFNRPLALIQCSTIHH
                     VSDAQRPHEIMERYIERLPAGSYLALTHFYDPQDGGDASELARFVEGVFSNSSMGSGF
                     FRTRREIESFFGDLELVEPGIARLRDWWPDGPHLNPPTAVDEVLIGGVARKP"
BASE COUNT         1551 a         3820 c         3765 g         1509 t
ORIGIN      
        1 acgagctcca cggcgcagcc gacctgggcc agcctgcgca cggtggcgcc gggcgggtag
       61 gcgatgcccg cgacccggtc gggcagctcc agcggcatct cgacggggac gggccgcggt
      121 gtggcgcccg cgtcggtacg cccgggggcg atcgtgccgc cgccgtgctt gacgagcagc
      181 tgcgcggccc gctcggccgc caccgggggc agctgcgggt cgacgacacg ctcgaaccgc
      241 ctggacgcct cgctgggcag cttgtgccgg cgcgcggccc tggcgatcac agcggggtcg
      301 aagtgcgcgg cctcgatgag gacatcgatg ctgcccgact cgccgggacc ggagatctcc
      361 gtggacgccc cacccatcac ccctgccagc ccgatcgggc cggagtcgtc ggtgatcaac
      421 aggtcgtcgg ggtccagggt gcgctcgacg tcgtccaacg tgcgtagctt ctcgcccgcc
      481 tcggcccggc gcaccacgat cgggccggac acccgggctg cgtcgtaggc gtgcaggggc
      541 tgaccgagct cgagcatcac gtagttcgtg acgtcgacga tcaacgagat cggccgcatt
      601 cccgcggcca gtaggcgccg ctgcatccac cacggcgccg gcgcggtggg gtcgaccccg
      661 tccacccggc gggcgacgaa ccgcgcgcac ccccggtcgg cgagctcgac cggccaggca
      721 tcgccgtacg cggccggtac cgcgaccctc gcggccgggt cggtgtagtc ggcgtccagg
      781 gacgcggcga gcacgcgggc caggccgcgc accgagaagc agtagccacg gtccggtgtg
      841 acactcagct cgatgaccgt gtccgggtcg tcgggcgcca ggccgagcag cggccgggcg
      901 tcgtcgccgg gttcggcggt ggcgggaggc agcaccagga tgccggcgtg gtcggcgccg
      961 attccgagct cccgggccga ggcaatcatg ccgtcggaga cgtgcccgta ggtcttacgg
     1021 gccgagatcg cgaacccgcc cggcagcacg gcgccgggca gcgcaacgac gacgaggtcg
     1081 cccgcctcga agttgccggc accacagatg atcccgcgcg actgggactc ccccacgtcg
     1141 acctggcacc accggatcgg cttcttcagc ccggtcagca cctcgaactc gagcacgcgg
     1201 ccgacgacca gcggaccggt cagctccggc gcccggtgga cgtcctcgat ctcgaggccg
     1261 acccggacga acgcctcggc cgtctcgtcg gcgggcggga cgtcgtcgag cagctcggcg
     1321 agccaggact gggaaacgcg cacgggaatg ctcctggggg gatcagatgc cggactcaga
     1381 tgccgaacgc gcggctgaac cggacgtcgc cctcgaccat gtcgcgcatg tcggggatgc
     1441 cgttgcggaa catcaacgtg cgctccaggc ccatgccgaa ggcgaagcca gagtagacgt
     1501 ccgggtcgac tccgcaggcg cgcagcacgt tggggtgcac catgccgcag ccgccccact
     1561 ccacccagcc cgcgccgccc ttcttctccg ggaaccacac gtcgggttcg gcggagggct
     1621 cggtgaacgg gaagaacgag gggcgcatgc ggatcttcga ttcggtaccg aacatcgagc
     1681 gcgcgaacgc gtcgagcgtg cccttgaggt gggccatcgt gaggcccttg gccacggcga
     1741 gaccctcgac ctggtggaac acaggggtgt gcgtggcatc gagggcgtcg gtgcggaatg
     1801 tgcggccggg gcacacgacg tacaggggca ggtcgcgctc cagcagggcc cggatctgca
     1861 ccggcgaggt gtgggtgcgc aggacggtgc ccgagtcctc ggagtcttcc cctgctgttg
     1921 atccggcgag gtagaacgtg tcctgcatgg tgcgcgccgg gtggtccttg ccgaagttca
     1981 gcgcatcgaa gttcaaccac gacgactcga cctcgggccc ctccgccacc tcccagccca
     2041 tgccgacgaa cacgtcggcg atgcgctcgg agagctgcgt gagcgggtgg cgcgcgccgc
     2101 gcgggatgcg gtcccacggc agcgtgacgt cgaccgtctc ctccacgagc acccgttcgt
     2161 cgcgctcggc gaccagcacc tcccggcggg cgtcgaacgc ctcctgcgct ctctgacggg
     2221 cggcgttgac acgcttgccg gcctcggccc gatccggccc cggcagcgag ccgagctcgc
     2281 ggcgcgccag cagcagcgga gcgcggtcgc ccaggtgggc gggcttgacg gcggccagcg
     2341 cagccaggtc ggaggccccc gcgaaagcct gaaccgccgc ggcgaccgcg gcgtccagcg
     2401 aatccgggtg aagcaggctc atcacgtccg tgctctcagt catggtgact cgatcctagt
     2461 gtcgaggccg gcggcgactc gcccacggtt gcccgccgcc gctgtgctgc gacactcgcg
     2521 tacaggcata ccgccgcggc cgtggccagg ttgaggctct cggcccggcc gtggatgggc
     2581 acccggacac ggtgctccgc ctgatcggcc acggtggccg ggacaccatg cgcctcgcca
     2641 ccgaatatcc atgcgacggg cccggcgagc acgtcgtcgg cggcggggtc gtgcaggtcg
     2701 aactcgccgt gggcgtcggc ggcgagcagc gtgagtccga ccgcgcggca ggcgtccagc
     2761 accgcggtgg cgtcgcggtg gcgcgccagc ggcaggtgga agacgctgcc ggtggaggcc
     2821 cggacggcct tgccgttgtg cgggtcgacg gtctcgcccg cgaacaggac ggcgtcggca
     2881 ccggcggcgt cggccacccg gatgaccgtg ccggcgttgc cgggatcggc aatgccggcg
     2941 cacacggcaa ccagcccggg ccggttcgtc agcgcggtgg gaagcggtac gtcgagcagg
     3001 tcacacacgg ccaccagccc ctgcggggtc accgtgtcgg acagcgactc ggcggcccgg
     3061 tcggtgacgc ggctgacccg cgcgccgatg tccgccgctg ccgagaccag ctcgggatgg
     3121 cgcgcggccg ccccgtcggt cacgaacagc tcgtgcaccc tgcccgaccg cggaccggcc
     3181 cactccacgg cctcgcggac ggcctgcgcc ccctccacga ggaaccgccc ggcccgatcg
     3241 cggcccgcgc ggcggagcag cttgcgagct gcgacgacac gcggcgtgcg ctgcgtcaat
     3301 gcgatctccg ctgacgctcc gatcggtgtg ccgggcggtt cggtcaccat cgcaccggac
     3361 ggacggctca ggccgcggtc ccgtcctgcg cgcccgtctg ggccggcacg ttggccctgg
     3421 ccacctcgac gagcgcggtg aaggctgccg ggtcgctgat ggcgagctcc gacaggatct
     3481 tgcggtcgac ctcgacaccc gaggccttga ggccctgaat gaagcggttg taggtcatgc
     3541 cgttggcgcg ggccgcagcg ttgatgcgag tgatccacag ctgccggaag tcgcccttct
     3601 tggcacgacg gtcgcggtag gcgtagttca gcgagtggag catctgctcc ttcgccttgc
     3661 ggtacaaccg cgagcgctgc ccgcggtagc cgctcgcaag ctccagagtg gtgcgacgct
     3721 tcttctgagc gttgaccgcg cgcttcacgc gtgccatggg gtgtccttac gtctatcggt
     3781 caggcgggtg gggtcagcgg tgtcgatccg cagtcagcgg ccgagcatct tcttgacgcg
     3841 cttgacgtcg gccttgttca cgggcacgac acgggagagg tcgcgggtct ccttgctcga
     3901 cttgacctcg aggcggtgcc gcaggccggc ctgctggcgc atgagcttgc cgctgccggt
     3961 caccttgacc cgcttggaca tcccactgtg cgtcttgttc ttgggcatga cagttccgtt
     4021 caccggtggc gccccggtag gggcgccaga ttggtcgtgc tgagcgttgc tgccgtcggg
     4081 cctgctacgc gtccgccgga gtagcgggcc gcgggcgggg tgccggcttc ttgttcggag
     4141 cgatcaccat cgtcatgttg cggccgtcct gcttgggcgc agcctcgacc gtgcccagct
     4201 ccgccacgtc ctcggcgagt cgctgcagca gccggaaccc cagctcgggg cgggactgct
     4261 cacgaccacg gaacatgatc gtgaccttga ccttgttgcc accctcgagg aagcgcacga
     4321 cgttgcgctt cttggtctcg tagtcgtggg tatcgatctt cggtcggagc ttctgctcct
     4381 tgatcgtggt cagctgctgg ttgcggcggg actccctggc cttctgcgcg ctctcgtact
     4441 tgaacttgcc gtagtccatg agcttgcaga ccggcgggcg ggcctgggcc gcgacctcga
     4501 cgaggtcgag ctctgcctcc tgcgcgagcc gcagggcgtc ctcgatacgc acgatgccga
     4561 cctgttcccc gttcggtccg accagccgga cctcgggaac gcggatgcgg tcgttgatgc
     4621 gcgtctctgt gctgatgggg tctcctcgta tcgacgtcac tgccgttttc agcagcgctg
     4681 cgcgcgacgg agtggagacc tgaacccgac acggtcacag ccgacgagcc tgcggctcgc
     4741 cggcgggacc ggacccggac acctgaggtg acgggtggga gcggggctcc acttgatcgc
     4801 cctgacgctc gtggaacgcg cgacagggtg gtcgcattcc gcaggatagc agcccgctca
     4861 cccgcctcgt gcgagacggg ggccggcgcg ccggtcggtc agggcgtcgt gctcccgtcc
     4921 ccccgcgcga gccgccgggc cagcgcgagc gcaccggagt cgcccatcgc cgcaccggca
     4981 gcatgcagcc cgtcgtactc ggccccgagc gccaccctgg ccgcatcctc cactgcccgg
     5041 atgcgcgccg agtcggcgcc gtactcggcg ctggcccggg ggctggcccg taccgccccc
     5101 agcaacatga cggcgtcccg gtgccggccg taccgggaca atgcctcggc caacgcacgc
     5161 accgcgaccc agagctgggt ccatgccccc atcccgtgcc agatgtcgag caatgggccg
     5221 aaccgcgccg gcgcctgggc cggatcaccg gcccgcgccg ccgtggtgag cagggtgtgc
     5281 cgcgcgacgc ctgccaggaa tgccgcgtcg acctcttccg ccagctcgac agcccgttcc
     5341 aggaacgctg ccgcacccgg ctccccggct tccgcccgcc gctcccccgc cgcgtacgcc
     5401 gcccaaccgc gcgcgagcga cgagcccatc cgctccgcga gcagcccggc cgccgcctcg
     5461 tgccgggctg cggctccgtc gtcgcctgcg taggccgtga tgatgacgag gtcggtcagc
     5521 gccattacga gtgtggcctc gtcgcccgcg gcctccgcca gctccgcagc accgcgcgcg
     5581 tactcgacgg cgtgcgcgag gtcgccgcgg aacatcagga tgttggccag cacttcgagg
     5641 gcgtcacgag cgagcagcgg gtcaccgagc cgctcgacga atgccagcgc acgccggcac
     5701 ctccgctcgg cgccctcgac gtcgccgcgc tgccagcgcg gcgcggccga gaggcccagc
     5761 agtcgcggta gcaacgggtg cagcgaggcc gcgccatcgg gctgacggtg gtcgggatcg
     5821 tcacggtccg gaccgccacg gtccggttca cagccggccg cccgcagcgc ggcgtcggcc
     5881 atctgcacga ggtcgacccg cgcccgctgg aagccgagct cggcacagac caggccgagc
     5941 cgcagaacgt cctccagcgg cccctccgtg cagagccatg cgtgcgcgcg ccggacgtcg
     6001 gcgaggtgcg cgtcgaaccg ccggacagcg gctggctcgt cgggccggga gcgcgccgcc
     6061 ccgacgtcga cggcgagatc caccgcccac cgggcgtgcc gtgcacgcaa cggcgccgcg
     6121 gccgggtcgg acgcgagttg ctcgcgggcg aacgcccgca gcgtgtcgag gatccggtaa
     6181 gcggtcggcc gcccgtcgcg tccctcctga cggacgacga gcgaacggtc caccaggtcg
     6241 ggcagagcag ctgtcgtgcc gcacacatcc gcgacggcgg cgtgctccac cgcgccggag
     6301 aacacaccga gccgcacgaa cagccgcctc tgctcgtcgt cgagcaggcc gaaggaccac
     6361 gcaacgacgt cgcgcagcga ccggtgccgg agcgccgcgg tgcggcggcc gtggcgcagc
     6421 acgtccaggg gctcctccag ggcgtcgagg agcccggcca gcccgactgc cggtacccgc
     6481 gcggcggcaa gctccagggc cagcgggagc ccgtccagcc gggcgcagat acgttgcacc
     6541 agagcggcgt cgacgctggt gggggtggca gcgaggcccg ccgcgctgat ccggtcggcg
     6601 agcagtacgg cggcgggacc cggcgccagg ggggcgaccg gcagcacctg ctcgccgtcg
     6661 acccgcagcg cctcccggct cgtcgcgagc acgtccaccc cgggcgcccc gatgaccacc
     6721 gcctcgacca gcgcggccac ggcatcggcg acgtgctcgc agtcgtcgag cacgagcagc
     6781 tgccggcgga cggcgagcac ttcgacgacg cggtccgtca ggctgccggg gccggcgtcg
     6841 gacagccgca gggcgcccgc gacggcggag acgaccgcgt ccgggcgggc cgaggcgagt
     6901 tcgaccatgg cggcaccgtc ggcgtaccgc cccgcgacgg cggccgcgac gtgtcgcgcc
     6961 agccgggtct tccccacccc acccgggccg cgcaatgtca cgacgcggcg cttcgcgagc
     7021 accgcgacgg cgcgggcgag gtcgccgtcc cgaccgacga acgagctgat cggcacgacg
     7081 gttgcggccg ggcggcgcgc gtgtgctgtg gggggtgccc ggccgggagc ttcgtgccgc
     7141 agcacctgct cgtgcagcgc gcgcagctcc ggcgacgggt ccaggcccaa ctgctcggcc
     7201 agctcgccgc gcagccggcc gtatgcacgc agcgcatcgc cgggtctccc cgcagccgcc
     7261 agcccgcgga cgaggatcgc gaccgcgcct tcccgcaacg gctcggcggc caccagcgct
     7321 tcggccgcgg ccaccgcctc accggtgcgg cctgcggcga gcaacgcctc cgcccgctgt
     7381 tcgacggcgg tggcccgcag gccggcgagg cgggcgacct cgggttcgac gtcggggtgg
     7441 tcgagctcgg cgaaaggccg gccacgccag agcgaaagtg ccgcatcgag ctcggcgagc
     7501 ctgcgtgccg gtccgggctc ctgcgcggcc cgggccagat gcgcggcgaa ccgcgcggca
     7561 tcgactccct cggggctgac gtcgagccgg taggtgcgcg ggccggtgcc gatcgcggca
     7621 ggcgccggca gcaaccgacg caggcgcgcg acgttggtct gcagcgcgcc gtcgggatcg
     7681 gccggcagct cgtcgcccca cacgagctcg gcaagcgtgt cgcaggcgac gtcggccccc
     7741 gcgtgcaggg cgagcgccgc gagcagcctg cgctggcggc cgcttccgag ccgcaccgtg
     7801 gccccgccgt cgtcctggag gacgaggggc ccgagcacgc cgacgaccgc acccgtgccc
     7861 tcggtgccgg ccatcctgcg attctcctgc agacaggagc tcatcgggca gaagaaccgc
     7921 cgtccatgcg ccctggcaga tgctgcagcg atgcgtggag tatcgcgact acggatcaac
     7981 ggatgcgaag gtcaccggat cggccgcaga cggtcagccg cgcgggtcca tgcaccactg
     8041 acggcgcggg tcgtcgccga gccgatgatt gcctcggcgg aaaagagcgc ggacaccgct
     8101 gaccaggcgc atcggagaat cgatgtcgtc tgccctgcgt acacccgccc cggcactgct
     8161 cgagggaagc gtcgcggcca acaggatcgc cctcacggtc gtcagtctgc cgaagactgt
     8221 atgcagatac aagaggcagc tgcacgtgca tcggcacgag tacgcacgtg atccccctcg
     8281 ccccggtcct ccctcggatt ctcgtgcgcg tgcctgggtg ctgaccagcg acgatggtcc
     8341 tcgaactacc gattggcaca gccgctgtgc tggttgacga ggcgccgcgc cgtggacgac
     8401 acctcggtgc ccgtgcacat gcacgtgcat ctgcgcgctg ctatggtccg atgaaggcaa
     8461 cgacgccgcc gagtccgtac gagctgggaa gaagacgtgg cgaacaacga ccgaaatggc
     8521 gggtcagcag tacagcttga tggcgctttc tggatgaaga gcagcctcag cggtgcagca
     8581 ggcaattgcg tggaagtggc cgcactggac agcggcgagt tcgcggtacg caactcgcgt
     8641 catcccaccg gccctgcgtt ggtcttcacc cgcgccgaat ggctggcatt cctgggcggt
     8701 gtggagcacg gcgagttccg tcctgcctga gctggaatcg gcgagcttcc ggatcagatc
     8761 tccgccagcg ctctcgccag gatctggcgc gatcgctcgg gggtatccgc gtcgaccgag
     8821 agtcggtcga ccgcccgact gtacagctct acctcgtcct gccggtcgag gtagagggcc
     8881 ccgctgagat gttcgacgta caccacatcc ggcagttccg gctcggcgaa ccggaggacc
     8941 gtgaacgacc cctcggcggc gtatccgctc aggtggtagg gcacgatctg aatagtgacg
     9001 ttggacagtt tcgagagttc gagtagctgc tcgagctgcg cgcgtagcac agctcggccg
     9061 ccgatcggtc gatgcaggac ggactcgtcc agcaccaccc acaacttcgg cgagttcatg
     9121 cccgtgagta tcttctgtcg ccgcatgcgc agcgtaactc ggcgctccgc gtcgtcagac
     9181 gcgtactcgg gtcggccgcg cgtcgcgatc gaccgggcgt aggcctccgt ctgcagcagg
     9241 ccggggacga actgcaactc gtaggtctgg atccgcgacg ccgactcctc cagacccacg
     9301 aaatcgtgga accaggtggg catgacatcg ctgtacctgc gccaccagcc cggctcgttc
     9361 gcctgcttga tcatctcgag gaaggcgtcg cgctccccgg ggtccgtgac gccgtacatg
     9421 gtcagaaggt cggagacgtc ccgctccttg aacgcaacgc ggccgagctc gagccgggac
     9481 accttggagt ccgacgagcg gatggcgtag ccggcgtcgg ctcgcgagat atgcgccgcc
     9541 tcccgcagcc gccgcaactg ggcaccgagc acaatgcgtc gcgccgtggc tcccctgccg
     9601 gactccccgt ccgcagaggt cggctcgcta cggccacctg cggccatcaa cgcctccgtg
     9661 cactatgcgg gggatgtgtc ccccacagcc caatggcaat ggagggttcg tgatacctca
     9721 gggctttcgt gcgaccccgc cgatcagcac ctcgtcaacc gcggtgggcg ggttgagatg
     9781 cgggccgtcc ggccaccagt cgcggagccg cgcgatgccg ggctcgacca gctcgagatc
     9841 accgaagaac gactcgattt cgcggcgcgt gcggaagaat cctgagccca ttgagctgtt
     9901 gctgaagaca ccctcgacga accgtgcgag ttcgctcgcg tcgccaccgt cctgcggatc
     9961 gtagaaatgg gtcagcgcca ggtacgagcc cgcgggcagt cgttcgatgt agcgctccat
    10021 gatctcgtgt ggccgctgcg catccgacac atggtgaatc gtgctgcact ggatcagggc
    10081 gagcggccga ttgaagtcga ggaactttgt gacgacaggg tgctcgagca cctggtccgg
    10141 ctcggtgaga tcggccatca ccaagtgcgt ctggtcgttc tcctccagca aagcccggcc
    10201 atgggccgcc accaccgggt cgttgtcgac gtacacgacc cgcgcctcgg gattcattcg
    10261 ctgagcgacc tggtgcgtgt tctcggccgt gggcagtccg gcgccgcagt cgagaaactg
    10321 atcgattccg gccgaacgag tgaggaatct cgttactcga accagccact cccgatggtc
    10381 gcgagccagc ctggacatct gcggagcagc cctgttgacc tgctcgaaca cctcgcggtc
    10441 gacatcgtag ttgtccttgc caccgaggaa acaatcataa acacgagcca cgctcgctct
    10501 ggtccggtca atggccgcgg agggcgccat cccaggttcg tcggctgctg cagagacgca
    10561 tctgaccgac ggctgggaac cggcgacacc acacgccgac tcgctgtgcc ggcgcttcgt
    10621 ccaccactgg gccgagcaga acgtc
//
LOCUS       DASRSC010000090         2406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4613201,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000090 DASRSC010000000
VERSION     DASRSC010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4613201"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="VFE39_04545"
     CDS             complement(<1..238)
                     /locus_tag="VFE39_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6257902.1"
                     /translation="MTDFEIPATGSAEVHVLHEGYVGLEGDGERVSGTVTLILDGDAV
                     IVVDPGMVADRGALLAALTAHGPRPEDVTDVVFSH"
     gene            complement(355..1200)
                     /locus_tag="VFE39_04550"
     CDS             complement(355..1200)
                     /locus_tag="VFE39_04550"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HET6257903.1"
                     /translation="MTATLRGDDPQHPPKPVADIGVIGGSGFYEFLTDAEEIRVETPF
                     GEPSEPVLVGEVEGRRVAFLPRHGKDHRFPPHKIPYRANMWAMRALGVRRVLAPSAVG
                     SLTVSYGPGTLVIPDQLVARTGGRVQSYYDQYAVHVPFADPYCPSGRAHAIATARKAG
                     WVPAGSGTLVVIDGPRFSTRAESRWYAAQGWTLVGMTGHPEAVLARELAVCYTTLALV
                     TDTDAGVDEGDGVTQAEVFEVFGKNMSRLRELVAKIIAAFPAEREDDLCAHALDGIRL
                     PFELP"
     gene            complement(1206..1574)
                     /locus_tag="VFE39_04555"
     CDS             complement(1206..1574)
                     /locus_tag="VFE39_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HET6257904.1"
                     /translation="MPTYQYVCTECGGQLEAVQKFTDESLTVHEDCGGRLRKVFSPVG
                     IVFKGSGFYKTDSRSSGSSGASTSTSSTSKENGSKENGSSSSSDSSSSSAKSGDSSSS
                     SSTAGSASSSSGGSSGEKVA"
     gene            complement(1634..>2406)
                     /locus_tag="VFE39_04560"
     CDS             complement(1634..>2406)
                     /locus_tag="VFE39_04560"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET6257905.1"
                     /translation="LGSNVHDMSASHTPREQQYAGQGDVANSDMAPGADNAQGTGKTD
                     LRRRLVAARAAMPDAERAAAGRLIRDQVLEMPEVSAAGTVAAYYSVGTEPDTHGLIFA
                     LWKRGSYVVLPVLLPDGDLDWASYEGPDSLAPGPRGLLQPVEPVRGPGTVARADVVLV
                     PALAVDRSGNRLGRGGGSYDRALARVGGQVPTIALIYDDELMPHVPTDPHDRAVRAVA
                     RPSHGITWLLSPGERPPVPPDCPRGRGVESADRTRSYP"
BASE COUNT          386 a          838 c          823 g          359 t
ORIGIN      
        1 ggtggctgaa gaccacgtcg gtgacgtctt ccgggcgagg cccgtgcgcc gtgagcgcgg
       61 cgagcagcgc gccccggtcg gcgaccatcc caggatcgac gacgatcacc gcgtcaccgt
      121 cgaggatgag ggtcaccgtc ccgctcacgc gctcgccatc gccctcaagc ccgacgtatc
      181 cctcatgcag cacgtgcacc tcggccgagc ccgtggccgg gatctcgaag tctgtcacga
      241 acagacccta tcgcggccgg gaacttcctg atatgcccga cctgtgaagt ctgaggatta
      301 cgtgaagccc gccgttggat tacgtgaagc ccgccgttcg ccgcgaagtc gcggttacgg
      361 cagctcaaac ggcaggcgga tgccgtcgag cgcgtgcgcg cacaagtcgt cctcgcgctc
      421 ggcggggaac gcggcgatga tcttcgccac cagctcgcgg agccggctca tgttcttgcc
      481 gaacacctcg aagacctcgg cctgtgtgac cccgtcgcct tcgtccacac ccgcatcggt
      541 gtcggtgacc agggccagcg tcgtgtagca gacggccagc tcgcgggcga ggaccgcctc
      601 gggatgcccg gtcatgccga ccagcgtcca gccctgcgcc gcgtaccacc gcgactcagc
      661 gcgggtagag aagcgcggcc cgtcgatgac gacgagcgtc cccgagccgg ccggcaccca
      721 gcccgccttc cgcgcggtcg cgatcgcgtg cgcccgaccg gacgggcagt acgggtcggc
      781 gaacgggacg tgcaccgcgt actggtcgta gtagctctgc acgcgtcccc cggtccgggc
      841 cacgagctga tcggggatga caagcgtgcc ggggccatag gacacggtga gcgagcccac
      901 cgcgctcggc gcgaggacac gccgcacgcc gagcgcgcgc atcgcccaca tgttggcgcg
      961 gtaggggatc ttgtgcggcg ggaaccggtg gtctttgccg tgcctcggca ggaacgccac
     1021 cctgcggccc tcgacctcgc cgacgagcac cggctcgctc ggctcgccga acggggtctc
     1081 gaccctgatt tcctcggcgt cggtcaggaa ctcatagaat ccggacccgc cgattacccc
     1141 gatgtccgcg accggcttcg ggggatgctg ggggtcgtcg ccccgaaggg tggcagtcat
     1201 cgcggttagg cgaccttctc gccggaggag ccgccggatg acgacgatgc ggagcccgcg
     1261 gtagaggacg aagaggacga gtcacccgac ttcgcagacg atgaagaaga gtcagacgag
     1321 gaagacgaac cgttctcctt cgacccgttc tccttcgacg tggacgaggt cgaggtcgaa
     1381 gcgccggacg acccgctgct gcggctgtcg gtcttgtaga agccggaccc cttgaacacg
     1441 atgcccacgg gcgagaagac cttgcgcagc cggccgccgc agtcctcatg caccgtaagc
     1501 gactcgtcgg tgaacttctg caccgcctca agctgaccgc cgcattcggt gcaaacgtac
     1561 tggtaagtgg gcacgattcc tcccaggcgg aacaaattcg gttcgctaca ttagcactcg
     1621 cagtagcaga ctgctaagga taactgcgag tgcggtccgc tgattccacg ccccgcccgc
     1681 gcggacagtc ggggggtacg gggggtcgtt cccccgggga taacagccag gtgatgccgt
     1741 gcgaggggcg ggcgaccgcg cggaccgcgc ggtcgtgcgg atcggtgggc acgtgtggca
     1801 tgagctcgtc gtcgtagatc agcgcgatcg tcggcacctg cccgccaacc cgggcgagcg
     1861 cccggtcgta ggatccgccg ccccggccga gccggttccc gctgcggtcg acggccaagg
     1921 cggggacgag gaccacgtcc gcacgggcca cggtgcccgg gccgcggacc ggctccaccg
     1981 gctggagcag gcccctcggc cccggggcga gcgaatccgg tccctcgtag gaggcccagt
     2041 cgaggtcacc gtcggggagc agcaccggca gtaccacgta gctgccgcgc ttccacagcg
     2101 cgaagatgag cccgtgcgta tccggctccg tgccgaccga gtagtaggcc gccaccgtcc
     2161 ccgcggcgga cacttccggc atttcgagca cctggtctct gatgaggcgc cccgcggctg
     2221 cccgctccgc atccggcatg gccgcgcgag ccgcgacaag ccgacgccgc aggtcggtct
     2281 tgccggttcc ctgggcgttg tcggcccccg gggccatatc agaattcgcc acgtcgcctt
     2341 gacctgcgta ctgctgttca cgcggggtat gtgaggcgga catgtcgtgc acgttgctcc
     2401 cgagaa
//
LOCUS       DASRSC010000091         5543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2320864,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000091 DASRSC010000000
VERSION     DASRSC010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5543
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2320864"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VFE39_04565"
     CDS             <1..667
                     /locus_tag="VFE39_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HET6257906.1"
                     /translation="GLPAGPYTMIVAAPGHEPLARTVAVGAGPATALGVLQLAREGLA
                     VLPAPGTWVIDPVHSSIHATALHIGMSRIHGRLRRFSGQLQVADPLENSSVEVLIDPT
                     AVDTDDDMRDQHLRSPDFLDVLRYAEIRYKSDGLRRVDPTHWVVEGVLTLKDVSAPVP
                     LQVEYRGTGPDPTGVVRAAFGATAEVSRDDFAINWNHSVLAGVLTIGRTLRIDIDIQA
                     MHT"
     gene            complement(675..1211)
                     /locus_tag="VFE39_04570"
     CDS             complement(675..1211)
                     /locus_tag="VFE39_04570"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET6257907.1"
                     /translation="MVLARTVVPAPEPIGALTLGLTTAGLALVVFGDRPEIVDRAATR
                     LGEAVVAGDGGTAAEQFAEYLAGTRRAFDMPLDWSLTSGSQRQVLQALYDTVPFGTTV
                     TYGELAARAWGRSERAYTAARGVGAIMGSNPLPVVVPCHRVLGSDSLGGFGGGRATKE
                     WLLALEGVLTPALDFGPG"
     gene            complement(1366..1995)
                     /locus_tag="VFE39_04575"
     CDS             complement(1366..1995)
                     /locus_tag="VFE39_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6257908.1"
                     /translation="MGGSTITRRRNTARSERAQATRARIVEAAGSQFVRDGYLETTMS
                     GIARAAGVAVQTLYLSFGSKVAVLEGALGAGSEDHPAGYTDQLTAEPDGILALGRYVA
                     VTVQAIERNHPLDVVLRAAAADPEPAELLERTRRAVLDQHAKAVDELAEKPGFTDRIS
                     LQRATDIVAAMLSHETYDLLVVGQGWTPPDWAEWAGRHLTIDLFPGNRP"
     gene            complement(2055..2720)
                     /gene="msrA"
                     /locus_tag="VFE39_04580"
     CDS             complement(2055..2720)
                     /gene="msrA"
                     /locus_tag="VFE39_04580"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HET6257909.1"
                     /translation="MGLFGRDKSQMITADQALPGRPTPLRVPTEHFVNGHRIQPPFPD
                     GLQTAVFGMGCFWGAERIFWQTPGVYSTAVGYAGGFTENPTYEETCSGLTGHTEVVLV
                     VFGPAQVSYETLLKLFWEGHDPTQGMRQGNDRGTQYRSAIYYADDAQRELAETTAKNY
                     GDALAASRFGEITTEIAPLAHFYYAEDYHQQYLAKVPDGYCGIGGTGVSCPVGTGVRS
                     DQG"
     gene            complement(3263..5050)
                     /locus_tag="VFE39_04585"
     CDS             complement(3263..5050)
                     /locus_tag="VFE39_04585"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020632756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HET6257910.1"
                     /translation="MSPTKHEQRGAHGAASSTHYLGTSTAMAVLARGGNAFDAAVAAG
                     FVLQVVEPHLCGPGGDLPAVFVTAGDSTPRVLCAQGVAPARATTALLRDELGLAIVPG
                     TGLLAPTVPGAWDGWLTMLRDHGTMSLAEVLEPALGYAASGFPLAPRIPTTIDTVADF
                     FREYWPTSAATWLPDGNVPTSVHRLPVLAATWQRLLDAAAAAGGDRERQIDAARDAWY
                     RGFVAEEIEAFCRTPVRDESGRDHAGLLTAEDLAGWSASYEDALTVDVGDGWTVAKAG
                     AWSQGPVLPQTLQLLHDGDLRYPDGVASAETVHQAVEAIKLAFADREAWYGDSAPVPL
                     ETLVSAEYAAARRALIGDRASLELRPGSPDGREPRLGAYAAKPGAKHGDGVSGPGLGE
                     PTISPTGAVRGDTVHVDVVDAAGNMVSATPSGGWLQSSPTIPALGFCLGTRGQMFWLE
                     DGLPSSLVPGRRPRTTLSPSLGLRDGEPRLAFGTPGGDQQDQWQLCFWLAHTVGGLDL
                     QAAIDSPAWHTTSFPSSFAPRGWEPGGLIAESRLGTATLDELRRRGHVVTDAGEWALG
                     RMCAVSRGDGPLLRAGADTRSGVGAAAAF"
     gene            complement(5152..>5543)
                     /locus_tag="VFE39_04590"
     CDS             complement(5152..>5543)
                     /locus_tag="VFE39_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HET6257911.1"
                     /translation="AENRWLLASARRFAGLPGVPPIADVEAFIADPCWVDLFAVVSDW
                     FLCAHGKGLKRIAPAAGFAWRDPEAGGENSMRWYRDAVGMDGAAPDEHQRRRLLEYNA
                     DDVAATRALREWMTSPAIDEVPLAAEL"
BASE COUNT          810 a         2031 c         1958 g          744 t
ORIGIN      
        1 cgggctgccc gccgggccgt acaccatgat cgtcgcggcg ccggggcacg agccgctggc
       61 ccgcaccgtc gcggtcggtg cgggcccggc caccgcgctc ggcgtgctcc agctcgcccg
      121 agagggcctc gcggtcctcc cggccccggg cacgtgggtc atcgacccgg tgcactcgag
      181 catccacgcc actgccctgc acatcgggat gagccgcatc cacgggcggc tgcgccgctt
      241 cagcggtcag ctgcaggtgg ccgacccgct ggagaacagc tccgtcgagg tgctcatcga
      301 cccgacggca gtggacacgg acgacgacat gcgggaccag cacctgcgca gcccggactt
      361 cctcgatgtg ctgcgctacg cggagatccg ttacaagagc gacggcctgc gccgcgtcga
      421 ccccacgcac tgggtggtgg agggcgtgct cacgctcaag gacgtgagcg cgccggtgcc
      481 gttgcaggtg gagtaccgcg gcacggggcc ggaccccacc ggcgtggtgc gcgccgcgtt
      541 cggggcgacg gcggaggtgt cgcgggacga cttcgccatc aactggaacc actcggtgct
      601 cgccggggtg ctgaccatcg gccgcacgct gcggatcgac atcgacatcc aggccatgca
      661 cacctgaacg tcgatcaccc cggcccgaag tcgagggccg gggtgagcac cccctcgagc
      721 gcgagcagcc actccttggt ggcccggccc ccgccgaacc cgccgaggga gtccgagccg
      781 agcacccggt ggcacggcac caccacgggt aacgggttcg accccatgat cgcgccgacg
      841 ccccgcgccg ccgtgtaggc ccgttccgac ctgccccatg cgcgcgctgc cagctcgccg
      901 tacgtgaccg tggtgccgaa cggcaccgtg tcgtacaacg cctgcagcac ctgccgctgc
      961 gaaccgctcg tgaggctcca gtccagcggc atgtcgaacg cgcgtcgagt accggcgagg
     1021 tactcggcga actgctcggc ggccgtacct ccgtcgccgg ccacgaccgc ttccccgagc
     1081 cgggtagcgg cccggtcgac gatctccggg cggtcgccga acaccaccag ggcgaggccg
     1141 gccgtggtga ggccgagtgt gagcgcgccg atcggctcgg gcgcggggac gacggtgcgg
     1201 gccaggacca cgaccccagt gtgtcctgct gcaccgacaa tgtcgcgccg ccctcgaacg
     1261 gccgatgact gctgccggag cgcagtgcgg tcgcatgatg accccgcgcg ggtcgtaaac
     1321 cgtacccccg caacagccgc tcacccggcg accggtcggt ctgggctacg gccggttccc
     1381 ggggaagaga tcgatggtca ggtgtcgccc ggcccactcc gcccagtccg gcggcgtcca
     1441 gccctggccg acgacgagca ggtcgtacgt ctcgtgcgac agcatcgcag cgacgatgtc
     1501 cgtggcccgc tgcagcgaga tccggtcggt gaagccgggt ttctccgcga gctcgtcgac
     1561 ggccttcgcg tgctggtcga gtaccgctcg ccgggtgcgc tcgagcagct cggcgggctc
     1621 gggatcggcg gccgcggcgc ggagtacgac gtcgagcgga tggttccgct cgatcgcctg
     1681 caccgtgacc gccacgtacc gacccagcgc gaggatgccg tcgggctccg ccgtgagctg
     1741 gtcggtgtac ccggccggat ggtcctccga gccggccccc agcgcgccct ccaggactgc
     1801 caccttactg ccgaaggaca ggtacagcgt ctgcaccgcg acgccggccg cccgcgcgat
     1861 gcccgacatc gtcgtctcca ggtagccgtc gcgcacgaac tgcgagcctg cagcttcgac
     1921 gatgcgggcg cgggtggcct gcgctcgctc ggagcgagcc gtgttgcgcc ggcgcgtgat
     1981 ggtagaacct cccactggaa cgacgttcca gtggaacact acaacatcat gttccggtgg
     2041 ccaggctgcc cgggtcagcc ctggtcggac cgcacccctg tgcccacggg gcaggagacg
     2101 ccggtgccac cgataccgca gtatccgtcc gggaccttgg cgaggtactg ctggtggtag
     2161 tcctcggcgt agtagaagtg ggcgagtggg gcgatctccg tggtgatctc gccgaagcgg
     2221 gaggccgcga gggcatcgcc gtagttcttg gcggtcgtct ccgcgagctc gcgctgcgcg
     2281 tcgtccgcgt agtagatcgc cgagcggtac tgcgtgccgc ggtcgttgcc ctgccgcatg
     2341 ccctgcgtcg ggtcgtgccc ctcccagaac agcttcagca gcgtctcgta gctgacctgc
     2401 gccgggccga acacgacgag gacgacctcc gtgtggcccg tgagcccgga acacgtctcc
     2461 tcgtaggtgg ggttctcggt gaacccgccc gcgtatccga cggccgtgga gtacacccct
     2521 ggcgtctgcc agaagattcg ctcggcaccc cagaagcagc ccatcccgaa caccgcggtc
     2581 tgcaggccgt cggggaacgg cggctggatg cggtggccgt tcacgaagtg ctccgtgggg
     2641 acccgcaacg gcgtcgggcg accgggcagc gcctggtcgg cagtgatcat ctgcgacttg
     2701 tcacgtccga acagacccat gagactcaga ttacgcggac gggcaggagt gcgaccgcga
     2761 tcgggcgggc gcccgaaccg ggtccctgtc acggaggcgg gggagccgtc gctgagagcg
     2821 gagcgcagca tcgggcgccg accaccgcag catcgccgct gcttcctcgc acggcaggcc
     2881 ggcgacgtcg acgagccaca cgaaggcctc gatgtcggtg gcggagcagt cccataaagg
     2941 ggaccgcgat gtgcgacagc gaacccgtca cgaccgatcc cggtctctac tccgtcgtgt
     3001 tcgagaacgg ccgcgtgcgg gttgcactat cgcgaccacc ccggcgaccg cacctcgctg
     3061 cactcccatc ccgaccggtg atcatccgtt gaccagcttc agccgccgct tcaccgtcgg
     3121 cgggcggacc agtcgaggtg gcgaagacgg cgcacgaggc gggatgggtg ccggcccagg
     3181 cgcacaaggg ggagaacgtg gcacgacgga gtcgaacgcg ctcttcgtcg aactgaagta
     3241 gcccctgcgc cggcacccgg ggtcagaacg ccgccgcggc gcccaccccg ctgcgggtgt
     3301 cggccccggc gcgcagcagc ggcccgtcac cgcggctcac ggcgcacatc cggcccagcg
     3361 cccactcccc cgcgtcggtg accacgtgac cgcggcggcg cagctcgtcc agcgtcgccg
     3421 tgcccagccg cgactcagcg atcagcccac ccggctccca gccccgcggt gcgaacgacg
     3481 aggggaacga cgtggtgtgc cacgccggcg agtcgatggc agcctgcagg tccagccctc
     3541 ccacggtgtg agcgagccag aagcacagct gccactggtc ctgctggtcg ccgccgggag
     3601 tgccgaaggc gagccgtggc tcgccgtcgc gcaggcccag ggaagggctc agtgtcgtgc
     3661 gcggacgcct gccgggcacg agcgaagacg gcaagccgtc ctccagccag aacatctgcc
     3721 ctcgtgtgcc gaggcagaac ccgagcgccg ggatggtggg cgaggactgc agccagccac
     3781 ccgacggcgt cgcggagacc atgttgcctg cggcgtccac gacgtcgacg tgcaccgtgt
     3841 cgccgcgcac cgcgcccgtg gggctgatcg tcggctcgcc cagcccgggc ccgctcaccc
     3901 cgtcgccgtg cttcgcgccc ggcttcgccg catacgcccc gagtcggggc tcacggccat
     3961 cgggcgagcc ggggcgcagc tcgagcgatg cccggtcgcc gatcagcgca cgccgagcgg
     4021 cggcgtactc cgcggacacc agcgtctcca gcggcaccgg tgcgctgtcg ccgtaccagg
     4081 cctcccggtc ggcgaacgcg agcttgatcg cctccaccgc ctggtgcacc gtctccgcgc
     4141 tggcgacgcc gtccgggtag cggaggtccc cgtcgtggag cagctgcagc gtctgcggca
     4201 gcaccggccc ctgcgaccag gcacctgcct tcgccaccgt ccagccgtcc ccgacgtcga
     4261 cggtcagggc gtcctcgtag gaggccgacc aaccggccag gtcctccgcg gtgagcaggc
     4321 ccgcgtggtc tcgaccggac tcgtcgcgca ccggtgtgcg acagaacgcc tcgatctcct
     4381 cagcgacgaa gccgcggtac caggcgtcgc gggcggcgtc gatctggcgc tcgcggtcgc
     4441 caccagccgc cgcggcggcg tcgaggagcc gctgccaggt ggcggcgagc accggcagcc
     4501 ggtggaccga cgtcggcacg ttgccatcgg gcagccaggt ggccgcggag gtcggccagt
     4561 actcgcggaa gaagtcggcg accgtgtcga tcgtggtcgg gatccgcgga gccagcggga
     4621 atccggaagc cgcgtaaccg agcgcgggtt cgagcacctc cgccagcgac atcgtgccgt
     4681 gatcgcgcag catggtcagc cagccgtccc atgccccggg cacggtcggg gccagcagcc
     4741 ccgtaccggg gacgatcgcc agaccgagct cgtcacgcag cagagccgtg gtcgcgcgcg
     4801 cgggcgcgac tccctgcgcg cagagcaccc gtggcgtgga atcgcctgcg gtgacgaaga
     4861 ccgcgggcag gtcgccaccc ggcccgcaca ggtgcggctc gacgacctgc agcacgaagc
     4921 ccgccgccac cgcggcgtcg aacgcgttgc cgccgcgggc gagcaccgcc atggcggtgg
     4981 acgtcccgag gtagtgggtg ctggatgcgg cgccgtgggc gcctcgctgc tcgtgcttcg
     5041 tcggcgacat gaaccagatc attgtcggca aggcgccgct ttgtcggacg agagcggcat
     5101 gaatcacgcc ttccgcgtga tcctcctgtc tcgagctcgg ccccctcccg ctcagagctc
     5161 ggctgcgagc ggcacctcgt cgatggcagg cgaggtcatc cactcccgca gggcgcgggt
     5221 ggcggcgacg tcgtcggcgt tgtactcgag caggcgcctg cgctggtgct cgtcgggcgc
     5281 agcgccgtcc atgccgacgg catcgcggta ccaccgcatc gagttctccc cacctgcctc
     5341 cgggtcacgc caggcgaagc ccgcggcggg cgcgatccgc ttgagcccct tcccgtgggc
     5401 gcacaggaac cagtcgctca caacggcgaa caggtcgacc cagcacgggt cggcgatgaa
     5461 cgcttcgacg tcggcaatcg gggggacgcc cggtagcccc gcgaaacggc gcgcggatgc
     5521 cagcaaccag cggttctcag cct
//
LOCUS       DASRSC010000092         8723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_356611,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000092 DASRSC010000000
VERSION     DASRSC010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356611"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..339
                     /locus_tag="VFE39_04595"
     CDS             <1..339
                     /locus_tag="VFE39_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET6257912.1"
                     /translation="TFADTRVWVERVSRAIGRTVPELVSWEWHKDRRDGLARLDYTQN
                     AINKTLIAPFSARPAPGAPVSVPLRWEELDDPDLRPDRWTIRTVIDRVEREGDPLASL
                     IGRDQRLPTL"
     gene            complement(404..1141)
                     /locus_tag="VFE39_04600"
     CDS             complement(404..1141)
                     /locus_tag="VFE39_04600"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6257913.1"
                     /translation="MLNGAATGTTQSVTVFRGDGARAGPAGRRRALAPAENRLSLRLI
                     AEPAAPSVVRGRIRSWLEGHGWPEDAIDDVVLAVHEAVANVVDHAYPPGATGKVAISA
                     SVLPDGRRRRRARVVVKDGGNWRPPPADPGYRGRGLQMVRGCMDVVDIHPGTDGRNGT
                     KLTMLTAAVGTTPADAGRHPRLRRLHHDLLDGVHRAQQRTLILACAARRLVDRVHRRL
                     TGWGVGPGRQPLRREDGTHRVRYVRMP"
     gene            complement(1152..1520)
                     /locus_tag="VFE39_04605"
     CDS             complement(1152..1520)
                     /locus_tag="VFE39_04605"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6257914.1"
                     /translation="MTELDNPRPRTWSWHSPTLRVVVSAHRNGASLRYNVRLVGELDL
                     ASVGSLTDALAALRDAQPELVVIDLSELQFVDARGLGALVEADRDLRAGGGSLVLARP
                     DPHVRRVLTLTAVDTQIEVR"
     gene            1720..2088
                     /locus_tag="VFE39_04610"
     CDS             1720..2088
                     /locus_tag="VFE39_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6257915.1"
                     /translation="MGVGRLDDEDYPAVAMGQAAELLDVQPAFLRSLDAAGVLQPQRS
                     GGGHRRYSRRQLVLAARMRTLFDEGLTLDAAARIVELQDGLDTARKRIAELEQQLARM
                     DDRQHGGDGGDVVDRAPPAQ"
     gene            complement(2119..2931)
                     /locus_tag="VFE39_04615"
     CDS             complement(2119..2931)
                     /locus_tag="VFE39_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6257916.1"
                     /translation="MPTALVTGASRGLGAVFAERLARDGFDLVLVARDRIGLEHTAAA
                     VRGNGMRADVLVADLADPAARATVEHRLADASRPVDVLVNNAGVENGNGFIQADLRDL
                     QAQIDTNITAVMSLTHAALPGMIARAGGSVINVASFAGYLSSPGNTYASTKAWVLAFT
                     DTMAASLPGTGVSMIALCPGRMRTGKHDAPKTPSPLWLQPAAVVDQCLRDLARGRSLC
                     TPGVVYRTVVGTLEMPRRTLRRLARLAGRGRAQRRGRAAQTHVRSAAVRIAA"
     gene            3508..4104
                     /locus_tag="VFE39_04620"
     CDS             3508..4104
                     /locus_tag="VFE39_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HET6257917.1"
                     /translation="MSAPKPLERLVLFTDAVAAIAITLLVLPLVDAVGTSVAARVPSV
                     DVITDNQPQIYSFLLSFAVIARLWIGHRRMFDHVDVDSRGLLLWNMLWLLTIVVLPFP
                     TQMIGAYGEDPFTPILYIGTILASSICLTVLAFIIRRGVDVSGAVSTTGLLVIAFVLA
                     ALVPGVRYFALLLLLLSPLADRVSEPLLRRAGVIPAER"
     gene            complement(4163..4996)
                     /locus_tag="VFE39_04625"
     CDS             complement(4163..4996)
                     /locus_tag="VFE39_04625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET6257918.1"
                     /translation="MARDQRRSVPPDGERSGARRDLNFTRPSLARVYDYVLGGREHFE
                     IDRRAAHGFLNVAPESAQVAKDSRNFLRRGVRYLVGEAGIRQIIDIGSGLPTTGNVHE
                     IAHEIDPTVHVVYVDKDPMVLTHARAMLGDDDTTTVISGDLRAPETIFDDPQTRALID
                     MDRPFAVLLSGILHHVSDKADPFRAAAEVSATLSPDSYLLISNFLDEDPRAKDLERAF
                     LDSVGSGRFRTWDENRRFFEGLEMVEPGLVYANRWRPDADTPVGNQVETLYVGGVGRK
                     V"
     gene            5325..6650
                     /locus_tag="VFE39_04630"
     CDS             5325..6650
                     /locus_tag="VFE39_04630"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6257919.1"
                     /translation="MSRTTRIVIVGGGFAGFHAARSLTRALPPHSGTEIVLVNPTDYF
                     LYLPLLPEVATAILDPRRLTVPLPAALPGVRLALGDVRSVSLEEHRISYTDPEGRNRS
                     LGYDRLVLAAGSVNKLMPIPGVAEHAHGFRGVAEALYLRDHIIRQLELAASTDDADER
                     DARCTFVVVGAGYTGTEVAAQGPLFTAAIADRHRELQDQKVRWLLLDLAPKVLPELDP
                     RLSRAADRVLRQRGVEVRMGTSVQEATTEGVRLTDGEFVPTRSLIWCVGVRPDPLIEE
                     TGLPTEKGRLVVEPTLVVPGHPDVFACGDAAAVPDLTRPGAVTAMTAQHATRQGTLAG
                     RNVAASLGYGSIRRYKHHDLGFVVDLGGTQAAANPLHIPLSGWPAKVVTRGYHLMAMP
                     GNRIRTAIDWILDLAVGRQSVQLGLVRSAAVPLDTASPELSRQPGGDSG"
     gene            complement(6838..>8723)
                     /locus_tag="VFE39_04635"
     CDS             complement(6838..>8723)
                     /locus_tag="VFE39_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6257920.1"
                     /translation="PLRAAIAEDLIVAHPDDFAFRHDLVREAVVANLPADLVRGLRRQ
                     VVDADLRTGRPLPEAAALLAAVALPGDTEAVSLLRQAASMVAASDPTAAADLSVRALE
                     LTPPRAAEWADLVTETVQLLLRAGRAAPAAALAEQALEIPLGPRAEARVRLGNALVGL
                     QFAFADAVRHCRIALELPQLPPSLRADLLAVLSLATALDGDPEAAGEVIGQAMTSARE
                     SGNARAEALTVCVGSIVDFHRTDWRTALAGADMAAALTKRVEVPQRLCEPEVWRVLAL
                     AGVGRLDEALAVADQHQAGVGVGLSLGRLWSATRSRLLLDAGRPAEARAAAETALSAV
                     DELGHGDFAAVTAITTLGRVAVLTGDEQALAAAERDAHKMQASRSASVRSAGAWLAAL
                     IADSGGDPGRAMAVLAEGASRFDDAGPWSGSPLDLADVPWFVRVALRAGHRDRAERAA
                     AAAGRRAEANPDVPLFVAAHAHARGLLLQDGEELVRAASLFEAGQRPLARASALEDAG
                     HVAAGRDREEAVRLFETAMRVNDEAGAQRDAARVRSALRELGVHRRRCTRPGTGQGWQ
                     ALTASELEVVRLIAQGATNRAAAERLYVSPHTVSTHLKHAFTKLGVTSRLELARIALT
                     RTQ"
BASE COUNT         1205 a         3120 c         3140 g         1258 t
ORIGIN      
        1 accttcgccg acaccagggt gtgggtggag agggtgtcgc gcgcgatcgg caggacggtg
       61 cccgagctgg tcagctggga atggcacaag gaccggcgcg acgggctcgc gcgtctcgac
      121 tacacccaga acgccatcaa caagacgctg atcgcgccgt tcagcgcgcg gccggcacca
      181 ggggcgccgg tgtccgtgcc gctccgctgg gaggagctgg acgacccgga tctgcgtccc
      241 gatcgctgga cgatccggac cgtgatcgac cgggtggagc gggagggcga tccgctcgct
      301 tccttgatcg gacgggacca gcggctgccg acgttgtagc agcactgtgc gagtggtcag
      361 ggacctgcag gaccgtggcg atcagcctcg agcgaccgca ccgtcagggc atccgcacgt
      421 accgcacgcg atgcgtgccg tcctcccggc gtagcggctg ccggccgggc ccgacgcccc
      481 agccggtcag gcggcggtgg accctgtcca ccaggcgacg ggctgcgcag gcgaggatca
      541 gggtccgttg ctgcgcgcgg tggacgccgt cgaggagatc gtggtgcagc cgccggaggc
      601 gcggatggcg gcccgcgtcc gcaggcgtgg tcccgacggc ggcggtgagc atggtgagct
      661 tggtgccgtt gcgcccgtcg gtacccggat ggatgtccac gacgtccatg cagccgcgca
      721 ccatctggag accgcgcccg cggtagccgg ggtcggcggg cggcggccgc cagttgcccc
      781 cgtccttgac caccacccgg gcccgccgtc ggcgccggcc gtcggggagc acggacgcgc
      841 tgatggcgac cttacccgtg gcgccggggg ggtacgcgtg atcgacgacg ttggccacgg
      901 cctcgtgcac ggcgaggacg acgtcatcga tggcgtcctc tggccagccg tgcccctcca
      961 gccaactgcg gatgcggccc cgtaccacgg agggagcggc tggttcggcg atcaggcgca
     1021 gggagagcct gttctcggct ggcgccagag ctctccgccg gcccgctggg ccggcacgag
     1081 ccccgtcacc tctgaaaaca gtcaccgact gtgttgttcc cgtggcggcg ccgtttaaca
     1141 cgtaggggag atcagcgcac ctctatctgg gtgtcgaccg ctgtgagggt caggacccgc
     1201 ctcacgtgcg gatccgggcg ggcgaggacg aggctgccac cgcctgcacg caggtcacgg
     1261 tcggcctcga ccagggcgcc gagcccgcgt gcatcgacga actgcagttc ggacaggtcg
     1321 atcacgacca gctcgggctg cgcgtcgcgg agcgcggcga gcgcgtcggt gagcgagccg
     1381 acgctggcca ggtcgagctc tccgacgagg cggacgttgt agcgcaggga ggcaccgttc
     1441 ctgtgggccg agacaacgac gcgcagggtc ggcgaatgcc aggaccatgt cctcggccgc
     1501 gggttgtcca gctcggtcac gggcgcgctc cctccgcgag ggtccctgta cgcgatggtc
     1561 tggccgcgca ggggctggtc acggctgtgg ctcaaccgag gcggatcgta ggacgggaac
     1621 gacccgcccg caacggtccg accagcgcgg acgcaacgaa ttgtggcgcc gagttgatag
     1681 gatttgtacc gctgactgaa acttggcagg gggctctcga tgggcgtggg ccggctcgac
     1741 gacgaggact atcccgcggt ggcgatgggc caggctgcgg agctgctcga cgtgcagccg
     1801 gcgttcctgc gcagtctcga cgcggccggg gtgctgcagc cgcagcgctc gggtggcggg
     1861 caccgtcggt attcgcgccg gcagctcgtg ctcgccgcgc ggatgcgaac cctgttcgac
     1921 gaggggctga ccctcgacgc ggccgcccgg atcgtcgagc tccaggacgg cctcgacacc
     1981 gcgcggaagc gcatcgccga gctcgagcag cagctcgcgc ggatggatga tcggcagcac
     2041 ggtggcgacg gtggcgacgt cgtggaccgt gcgcctcctg cgcagtgacg agtgcggggt
     2101 aggcgtgccg gctggggttc aggccgcgat gcggacggcc gccgaccgga cgtgcgtctg
     2161 cgcggcgcgt ccgcgtcgtt gtgcgcggcc gcggcccgcg agccgcgcca gccgacgcag
     2221 cgtgcggcgc ggcatctcca gcgtgccgac cacggtgcgg tagaccaccc cgggcgtgca
     2281 gaggctgcgg ccgcgggcga ggtcgcggag gcactggtcc accacggccg cgggctgcag
     2341 ccagagaggc gacggcgtct tgggggcgtc gtgcttgccg gtgcgcatcc gacccgggca
     2401 gagggcgatc atgctgaccc cggtgccggg cagcgacgcc gccatggtgt cggtgaacgc
     2461 caggacccag gccttcgtcg acgcgtacgt gttgcccggc gaggagaggt agccggcgaa
     2521 gctcgcgacg ttgatcacgg atccgccggc gcgggcgatc atgccgggga gagcggcgtg
     2581 ggtcagcgac atgaccgccg tgatgttcgt gtcgatctgg gcctggaggt cgcgcaggtc
     2641 ggcctggatg aacccgttgc cgttctcgac gccggcgttg ttcaccagca cgtcgacggg
     2701 ccggctcgca tcggcgagcc ggtgctccac ggtcgccctg gccgccggat cggcgaggtc
     2761 tgcgacgagg acgtcggccc tcatgccatt cccacgcacc gctgctgcag tgtgctccag
     2821 cccgatccgg tcgcgcgcga cgagaacgag gtcgaagccg tcacgggcga ggcgttcggc
     2881 gaagacggcg ccgaggccgc ggctggctcc ggtgactagt gcggtgggca tgggtcaaac
     2941 gtagggacgg cagcctgtag cgacgctgag aaaccggccc gaacctgact gacggctgag
     3001 gacggcgatc cgcgaggtgc gctcgagcgt tacgacattc gacgccgccg agatgatctg
     3061 acgtcacacc gttccgcgtg gcctctcccg cgctcacgac cgtcgccgcc accgcacggg
     3121 cacggacccc gttgccctga ccgacgggta cgcactcggg ctcacgatct ccggcgctct
     3181 gctggtcgtt gccgtgatcg tggcactaat agtccccggt acctgccggc aggctcgtcg
     3241 tcgttccgcg cgcccctgga gctggacggc ccgagcaact tgatgtccat caccctgcgg
     3301 cacccaactg gaggagcgcg gcgcggaaac cgccgaccgc ccgccggacc gatgcagctc
     3361 gcgccgcgca gttccccgag tgggctgacg tgccggtcgt ccggtcgatg tccacgggtg
     3421 ggacaaccgc gctttccgcc tcggagaggc gatgacggtc cagctcccga ccgccgaggg
     3481 ttacgtgcag aactctatgt tgacagcgtg agcgcgccca agccgctgga acggctcgtc
     3541 ctgttcaccg atgccgtggc cgcgatcgcg atcacgctgc tcgtgctgcc gctggtcgac
     3601 gcagtgggga cgtcggtcgc cgctcgggtg ccctcggtgg acgtgatcac ggacaaccag
     3661 ccgcagatct acagcttcct gttgagcttt gccgtcatcg cgcgcctctg gatcggccac
     3721 cgccgcatgt tcgaccacgt ggacgtcgac agccgcgggc tgttgctctg gaacatgctc
     3781 tggctgctca cgatcgtggt gctgcccttc ccgacccaga tgatcggcgc gtacggcgag
     3841 gacccgttca cgccaatcct ctacatcggg acgatcctcg cgagcagcat ctgcctgacc
     3901 gtgctcgcgt tcatcatccg ccgcggggtg gacgtgagcg gcgcggtgag cacgacggga
     3961 ctgctggtga tcgcgttcgt gctggcggcc ctggttccgg gtgtgcgcta cttcgcgctc
     4021 ctgctgctcc tgctctcccc tcttgcagac cgcgtcagcg aacctctgct gcgccgcgcc
     4081 ggagtcatcc cggccgagcg gtgagcccgc ttctgcagca ccgtcgcgcc accggcgcag
     4141 caaggtagct gcgctgcccg cgtcagacct tccggccgac cccgcccacg tagagggtct
     4201 cgacctggtt gccgacgggc gtgtcagcgt ccggccgcca cctgttggcg tacaccaggc
     4261 ccggctcgac catctcgagt ccttcgaaga agcgccggtt ctcgtcccat gtccggaagc
     4321 gccccgagcc gacgctgtcc aagaaggcgc gctcgaggtc cttcgcgcgc gggtcctcgt
     4381 cgaggaaatt actgatcagg aggtagctgt caggggacag cgtggcgctg acctccgccg
     4441 cggcgcggaa gggatcggcc ttgtcggaga cgtggtgcag gatgcccgaa aggagaacgg
     4501 cgaacggccg gtccatgtcg atgagcgcgc gggtctgcgg gtcgtcgaag atcgtctcgg
     4561 gggcccgcag gtcgccgctg atcacggtgg tcgtgtcgtc gtcgccgagc atggcccggg
     4621 cgtgcgtcag caccatcgga tctttgtcga cgtagacgac gtgcacggtg gggtcgatct
     4681 cgtgcgcgat ctcgtgcacg ttccccgttg tcggcaggcc ggagccgatg tcgatgatct
     4741 gccggatgcc cgcctcgccc accagatagc gcacgcctcg gcgcaggaag ttccggctgt
     4801 ccttggcgac ctgtgccgac tccggcgcca cgttcaggaa gccgtgggcg gcccgccggt
     4861 cgatctcgaa gtgctcccgc ccgccgagaa cgtagtcgta gacgcgcgcg agcgagggcc
     4921 tggtgaaatt caggtcgcgg cgcgcgccgc tgcgctcgcc gtcgggcggc accgatcgtc
     4981 gctgatcacg cgccacggaa tcctcccttg cgggccggtc acccactggc cgagtcccgt
     5041 cgtcgatgtt cgcggcggaa gactagcctg atcaacagca cgttcggcag agcctgccgg
     5101 cgtcagcatg ccgtgtagtg gtccaaccca gccccgacga cctcccggat cagctcgaac
     5161 cgcacctctg cgccgtcggc ggtggcgaac ctgacctcgt gggactggcc gacggagagc
     5221 tcgtcgaggg gccggtggaa gcggaggggg gccagaattg tcgcgacgga gatcggcggt
     5281 cgtccggccc cgcgtcggac gcgcctgacg gaaggatgcc ggagatgagc aggactactc
     5341 gcatcgtgat cgtcggcggt ggattcgccg ggttccacgc ggcccgctcg ctcacccgtg
     5401 cactcccgcc gcactcgggc acggagatcg tgctggtgaa ccccacggac tacttcctct
     5461 acctgcctct gctgccggag gtcgccacag cgatcctgga tccgcggcgc ctgacggtgc
     5521 cgctgccggc cgccctgccc ggggtgcgtc tcgcactggg cgacgtgcgg tcggtgtccc
     5581 tcgaggagca ccggatctcc tacaccgacc ccgagggccg caaccgcagc ctcggctacg
     5641 accggctggt cctcgccgcc ggcagcgtga acaagctgat gccgatcccc ggtgtcgccg
     5701 agcacgccca cggcttccgt ggggtggccg aggcgctcta tctgcgtgac cacatcatca
     5761 ggcagctcga gctcgccgct tccaccgacg acgccgatga gcgcgacgcc cggtgcacgt
     5821 tcgtcgtggt cggggcgggc tacaccggca ccgaggtggc cgcgcagggt ccgctgttca
     5881 ctgccgccat cgccgaccgc caccgggagc tgcaggacca gaaggtgcgc tggctcctgc
     5941 tcgacctcgc accaaaggtg ctcccggagc tggacccgcg cctctcccgg gcagcggacc
     6001 gggtcctccg gcagcgcggc gtggaggtgc ggatgggcac ctccgtgcag gaggcgacca
     6061 ccgagggcgt ccgtctcacc gacggcgagt tcgtcccgac gcggtccctc atctggtgcg
     6121 tgggggtccg gcccgatccg ctgatcgagg agacgggtct cccgacggag aagggccggt
     6181 tggtggtgga gcccacgctc gtcgtgccgg gccatcccga tgtcttcgcc tgcggggatg
     6241 ccgctgcggt gcctgacctg acgcggcccg gcgcggtgac cgcgatgacc gcgcagcacg
     6301 ccacccgtca aggcacgctc gccggccgga acgtcgctgc gtcgctcggg tacggcagca
     6361 tccgccgcta caagcaccac gatctcggtt tcgtggtcga cctcggcgga acgcaggccg
     6421 cggccaaccc gctgcacatc ccgctgtccg ggtggcccgc caaggtcgtc acccgcggct
     6481 accacctgat ggccatgcca ggcaaccgca tccgcacggc gatcgattgg atcctcgacc
     6541 tcgccgtggg tcgccagtcc gtccagctcg ggctggtgcg ctcggctgcc gtgccgctcg
     6601 acacggcgtc gcccgagctg tcgcgtcaac caggcgggga ctccggctga ctgtcggaga
     6661 tccattacct caacgatcca tcaccgcaac cacgtcacga tgagggtggg tcccccaaca
     6721 cgtgatgttg gccgccccgc ggctcgagcg ctaggccacc gccacgatgc gaccgccgtg
     6781 agccctgcac cccccagccc gcccctgggc cacagggcag ggagccaccg accccgttca
     6841 ctgcgtgcgg gtcagggcga tgcgggcgag ctcgagccgc gacgtgacgc cgagcttggt
     6901 gaatgcgtgc ttgaggtggg tgctcacggt gtgcggcgag acatacagcc gctcggcggc
     6961 ggcccggttg gtggcgccct gcgcgatgag ccgcaccacc tccagctcgc tcgccgtcag
     7021 cgcctgccat ccctggccgg tgcccggccg cgtgcagcga cggcgatgta cacccagctc
     7081 gcgcagcgcc gatcggacgc gcgcggcgtc gcgctgcgcg cccgcctcgt cgttgacgcg
     7141 catcgcggtc tcgaacaacc gaacggcctc ctcgcggtcc cgtccggccg cgacgtggcc
     7201 tgcgtcctcc agcgcggagg cacgcgccag gggccgttgc ccggcctcga acagcgacgc
     7261 cgccctgacc agctcctcgc cgtcctgcag gagcagcccg cgggcgtgcg cgtgggcagc
     7321 gacgaacaga ggcacgtccg ggttcgcctc ggcccgacgt cccgcggcgg cggccgcccg
     7381 ttcggcgcgg tcccggtggc cggcccgtag cgcgacccgc acgaaccacg gcacgtcggc
     7441 gaggtcgagc gggcttccgg accacgggcc cgcgtcgtcg aatcggctgg ccccctcggc
     7501 gaggaccgcc atggcccgac ccgggtcacc gccggagtcg gcgatgagag cagccagcca
     7561 ggcgcctgca ctgcgtacgg acgccgacct gctcgcctgc atcttgtgtg cgtcccgttc
     7621 cgctgcggcc agcgcctgct cgtcgcccgt gagcaccgcc acccggccga gcgtcgtgat
     7681 cgcggtgacc gcggcgaagt ccccatgacc gagctcgtcg accgcgctga gcgccgtctc
     7741 ggccgcagcg cgtgcctcgg caggccggcc ggcgtccagc agcagccgcg agcgggtcgc
     7801 gctccacaac cgccccagcg acaggccgac ccccacgccg gcctgatgct ggtccgcgac
     7861 ggccagcgcc tcgtcgagcc ttccgacgcc ggccagcgcc agcacgcgcc acacctccgg
     7921 ctcgcacagg cgctgcggca cctccacccg cttcgtcaaa gcggccgcca tgtcggcccc
     7981 ggcgagcgcc gtgcgccagt ccgtccggtg gaagtcgacg atggacccga cgcacaccgt
     8041 gagcgcctcg gctcgcgcgt tgcccgactc gcgggcagag gtcatcgcct ggccgatgac
     8101 ctcgccggca gcctccggat ctccgtcgag cgcggtggcc agcgacagca cggcgaggag
     8161 gtcggcccgc agcgacggtg gcagctgcgg cagctccagg gcgatccggc agtgccggac
     8221 cgcgtccgcg aacgcgaact gcaggccgac cagtgcattg cccagccgta cccgagcctc
     8281 ggcccgcggc ccgagcggga tctcgagcgc ctgctccgcg agcgcagcgg ccggagctgc
     8341 acggccggcg cggagcagca gctggacggt ctcggtcacc aggtcggccc actccgccgc
     8401 gcggggcggc gtgagctcga gggcccgcac gctgaggtcc gccgcggccg tcgggtccga
     8461 tgctgcgacc atcgatgcgg cctgccgcag cagcgacacc gcttcggtgt cgccgggcag
     8521 ggccaccgcg gcgagcaggg cagcggcctc cggcagcggt cgacccgtcc gcaggtccgc
     8581 gtcgacgacc tgtcggcgca gcccccggac cagatcggcg ggcaggttcg cgacgaccgc
     8641 ctcgcggacg aggtcgtgcc ggaaagcgaa gtcgtcgggg tgcgcgacga tcaggtcctc
     8701 ggcgatggcc gcgcgcagcg gct
//
LOCUS       DASRSC010000093         5583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2814114,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000093 DASRSC010000000
VERSION     DASRSC010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2814114"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VFE39_04640"
     CDS             complement(<1..463)
                     /locus_tag="VFE39_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6257921.1"
                     /translation="MEFRYLGDSGLKISEITYGNWLTHGSQVENDAATACVRAALDAG
                     ITTFDTADAYANGAAETVLGEALKGQRRASLEILTKVYWPTGPKGPNDSGLSRKHIME
                     SIDGSLSRLQTDYVDLYQAHRYDSETPLEETMQAFADVVRAGKALYIGVSEW"
     gene            complement(641..943)
                     /locus_tag="VFE39_04645"
     CDS             complement(641..943)
                     /locus_tag="VFE39_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HET6257922.1"
                     /translation="MLAITDNAAEAIKTLTTDAELPDGGGLRISAPDPDQGLELSLAG
                     QPDVDDVVLAGEGVSVFLEPVAAEVLDDKVLDVQPVPGADGEQELRFAIGLQQPEA"
     gene            complement(1008..1835)
                     /locus_tag="VFE39_04650"
     CDS             complement(1008..1835)
                     /locus_tag="VFE39_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6257923.1"
                     /translation="MSRVPKIRLNNGVGIPQLGFGVFQIEPDRTAEAVLTAFEAGYRH
                     IDTAQGYNNEEGVGEAIRASGLRRGEIFVTTKLANDRHGYDEAISALDESLQRLGLDH
                     VDLYLIHWPRPAQNRYVETWKAFEKIASDGKARAIGVSNFHIPHLERLAQETATVPAV
                     NQIELHPWLPQTELRAYHQRHGIATEAWSPLAKGGELLADELIVGLAQKYGKTPAQIV
                     LRWHLQLGNIAIPKSVTPSRVRENADVFDFELSDDDVASISELGNGRRLGPDPDNFG"
     gene            complement(1867..2706)
                     /locus_tag="VFE39_04655"
     CDS             complement(1867..2706)
                     /locus_tag="VFE39_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6257924.1"
                     /translation="MTDRALVLGGGGITGIAWELGLIAGLAERGLDLTGADLLVGTSA
                     GSVVGAQIATGVDVEERYAAQLVPPTGEIAAALGRGTIVRFGLAMIGSRDPQRVRARI
                     GRLALKARTVPEAERIRVIGERLPVHEWPERALRIATVDAHTGEFVILDRDAGVPLVE
                     AVAASCAVPGVWPPVSTGGRRFIDGGVRSPTNADLAAGFSRVVVIAPIVRGIGPLVGV
                     DAQVAALRADGSLVTVVSPDAAAVAAIGRNVLDPVRRAGSARAGRAQAAAAADAVGAV
                     WSS"
     gene            complement(2703..3392)
                     /locus_tag="VFE39_04660"
     CDS             complement(2703..3392)
                     /locus_tag="VFE39_04660"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET6257925.1"
                     /translation="MPGTALPDPAVTRTLAVLGLVGAGLAVVLVGMLHVVAADQVNPV
                     RRTISEYALGESDWMFDAGVLGLSVGSALVLLALVRAGILRPWSGAAVLLAVWAVALV
                     IVVAFDKANWSVGPSMSGYVHRYASLAAFASLPVAALALGRRWRGDTLWGSFAAWSRW
                     AGVLALLWLGSILLAVAVRPLTGAPWWQLLPLGLVERGLAITEVAVLVVLGRWAVRAA
                     AVPQPAEMISA"
     gene            complement(3806..>5583)
                     /locus_tag="VFE39_04665"
     CDS             complement(3806..>5583)
                     /locus_tag="VFE39_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6257926.1"
                     /translation="IREDGAGIVTRYVDGGEAWPGWEDAAVPPERFGAYLREFDALLT
                     RHRRRGAYYGHFGDGCLHVRIDFDLLTRSGIADFRSFMEDAADLAVAHGGSLSGEHGD
                     GAARAELLPRMYPPEIIAAFEEFMGIWDPDDRMNPGRVVRPAKLDEDLRVFVGMPTLP
                     DEPELAFAHDRGSFTRATRRCLGVGKCVTADGGVMCPSYRATGEEMHSTRGRARLLFE
                     MAAGDVITGGWQSPEVAEALDLCLSCKGCKRDCPVGVDMAAYKTEFLAQRYRRKVRPA
                     SHYSMGALPRWMRLVGRLPARMVDALNAAARGRLGAYAKKAGGITPERPIPAIARRPF
                     TAVRSPRGPETGAPRGRVLLWPDTFTNHFDPGIADAAVAVLEGLGYAVELPPRAVCCG
                     LTWTSTGQVDAARRVLRRSLRTIEPWLAAGVPVVGLEPSCTAALRSDGPELLPDEPLA
                     AALSSGVRTFAEVLAQHADELRDRVVERGRRALVQVHCHQHAELGTDDDRAVLTALGV
                     DAEVLDSGCCGLAGNFGFEKGHYEVSMACAERVLLPAVRAADPDVAVLADGFSCRTQL
                     RQAGTREPMHLAQLAAQALGLTPTP"
BASE COUNT          833 a         2072 c         1906 g          772 t
ORIGIN      
        1 tccactcgct gacgccgatg tagagcgcct tgccggcgcg cacgacgtcc gcgaaggcct
       61 gcatcgtctc ctcgagcggc gtctcggagt cgtagcggtg cgcctggtag aggtcgacgt
      121 agtcggtctg cagccgggac aagctcccgt cgatcgactc catgatgtgt ttgcgggaca
      181 acccgctgtc attgggcccc ttcgggccgg tgggccagta gaccttcgtc agaatctcca
      241 gcgacgccct gcgctggccc ttcagcgcct cgccgagcac ggtctcggca gctccgttcg
      301 cgtaggcgtc ggcggtgtcg aacgttgtga tcccggcgtc cagagccgcg cggacgcagg
      361 ccgtggcggc gtcgttctcg acctgtgagc cgtgcgtgag ccagttgccg tacgtgatct
      421 cggagatctt caggccggag tcacccagat aacgaaattc cacagttcga ctgtagatgc
      481 cggacaggag cccgccgcca ccggttcgcg ggttcctggt cacaccggtt cgtgagctgc
      541 cgcggcgact cggcaccgct cgcccgtgcc cggaaagacg atgggtggca ccaccgactc
      601 cagtgtcggc cgtgccaccc ggtagctggt ggggcggggg tcaggcctcc ggctgttgca
      661 gcccgatggc gaagcggagt tcctgctcgc cgtccgcgcc cggaaccggc tggacgtcga
      721 gcaccttgtc gtcgaggacc tccgccgcca ccggctcgag gaacacgctc acaccctcac
      781 cggcgaggac gacgtcgtcg acgtccggct gaccggcgag cgacagctca aggccctgat
      841 ccgggtcggg ggcggaaatt cgcaggccac ccccgtcggg aagttccgcg tccgtcgtca
      901 gggtcttgat ggcttcggcc gcgttatcgg tgattgccaa catgcggcgc aacgctaggg
      961 gcgcgaggcc gcgcccgcag cgtggagccg ccgaccatgg gtagtgatca gccgaagttg
     1021 tcggggtccg ggcccagccg cctgccgttc ccgagctcgc tgatgctcgc tacgtcgtcg
     1081 tcggacagct cgaaatcgaa gacgtcggcg ttctcacgga cgcgcgatgg cgtcaccgac
     1141 ttcgggatcg cgatgttgcc gagctgaaga tgccagcgca gcacgatctg ggccggggtc
     1201 ttgccgtact tctgggcgag tccgacgatc agctcgtcgg ccagcagctc gccacccttg
     1261 gcgagcgggc tccacgcctc ggtcgcgatc ccgtgcctct ggtggtaggc gcggagctcg
     1321 gtctgcggga gccacgggtg cagctcgatc tggttcaccg cgggcaccgt ggccgtctct
     1381 tgggcaaggc gctcgaggtg cgggatatga aaattggaga cgccgatcgc acgcgccttg
     1441 ccgtccgacg cgatcttctc gaatgccttc cacgtctcca cgtagcggtt ctgagccggc
     1501 cggggccagt ggatcaggta gaggtcgacg tggtcgagcc cgagccgctg gaggctctcg
     1561 tcgagggcgc tgatcgcctc gtcgtagccg tggcggtcgt tggccagctt ggtggtgacg
     1621 aagatctcgc cgcgccgcaa cccggaggcc cggatcgcct ccccgacgcc ttcctcgttg
     1681 ttgtagccct gtgccgtgtc aatgtgccga tagccggcct cgaacgccgt caggacggct
     1741 tccgccgtcc ggtccggctc gatctggaaa acgccgaatc ccagctgcgg gatcccgacg
     1801 ccgttgttca gacgaatctt cggtacgcgc gacatgaccg cgaggctacc caccacgtcg
     1861 caggcgttaa ctgctccaga ccgcgcccac cgcgtcggca gctgcagcgg cctgcgcacg
     1921 cccggctcgc gccgaccccg ccctgcgcac ggggtcgagc acgttgcggc cgatcgccgc
     1981 caccgccgcc gcgtcggggc tcaccaccgt gaccagcgaa ccgtccgctc gcaatgccgc
     2041 cacctgtgcg tcgacgccga ccagcggccc gatcccccgc acgatgggtg ctatcacgac
     2101 gacccgggag aacccggcgg cgaggtcggc gttggtgggc gagcgcacgc cgccgtcgat
     2161 gaagcggcga ccgccggtgc tcaccggggg ccagaccccc ggcaccgcac agctcgctgc
     2221 cacggcctcc accagcggca ccccggcgtc gcggtcgaga atgacgaact cgccggtgtg
     2281 cgcgtccacc gtcgcgatgc gcaacgcccg ctccggccat tcgtgcaccg gcagccgctc
     2341 cccgatcacc ctgatccgct cggcctccgg aacagtgcgg gccttcaacg ccagcctgcc
     2401 gatccgggcg cgcacccgct gcgggtcccg ggacccgatc atcgcgagcc cgaaccgcac
     2461 tatcgtgccc cggcccaacg cggccgcgat ctcgccggtg ggtggcacga gctgggcggc
     2521 gtaccgctcc tccacgtcga cgccggtcgc gatctgggcg ccgaccacgg acccggccga
     2581 cgtcccgacg agcagatctg caccggtgag gtcgaggcct cgctcggcca gacctgcgat
     2641 cagccccagc tcccacgcga tccctgtgat gccgccgcca cccaggacga gcgcccgatc
     2701 agtcatgcag aaatcatctc agccggctgg ggcaccgcgg ccgcgcgcac agcccaccgt
     2761 ccgagcacca cgagaacggc gacctccgtg atcgccaacc ctcgttccac cagtccgagc
     2821 ggtaggagct gccaccacgg cgcgccggtc agcgggcgca cggcgacagc cagcaggatc
     2881 gagccgagcc acagcagcgc gagtaccccc gcccaccgcg accaggcggc gaacgagccc
     2941 cacagcgtgt cgccgcgcca acgcctgccg agcgccagcg ccgccacggg caggctcgcg
     3001 aacgcagcca ggctcgcgta gcggtgcacg tagccgctca tgctcggccc cacggaccag
     3061 ttcgccttgt cgaacgcgac gacgatcacc agcgccaccg cccagaccgc cagcaggact
     3121 gctgcgccgg accacggacg caggatccct gcacgcacca gcgcgaggag cacgagcgcc
     3181 gagcccaccg agaggcccag cacaccggcg tcgaacatcc agtcggactc gccgagggcg
     3241 tactcgctga tcgtgcgccg aaccgggttc acctgatccg ccgcgacgac gtgcagcatc
     3301 cccaccagca ccaccgcgag gcccgcgccc accagcccga gcaccgccaa cgtcctcgtc
     3361 accgcgggat cgggcagcgc agtaccgggc acggacatcc tcctggggag cacggggtca
     3421 ggaggtcaac gactgccagc gaggagacgt tcctcacacg gcacctcggc gagggcggga
     3481 gattcgcgcg aaccggataa taacgccctc actgcggccc tgaagtccgg ctcgctccga
     3541 tcatggggcc gccgggccat aagggccccc gcacgctctc gggcggaacc cgggtgactc
     3601 gcgagccgat tccgagcgac ttgcgggcgg ttttcgggcg actcgccgac acattcccgc
     3661 gcgactcgcg ggcggaactc gcggcgactc gcggacggat tccaggcgac tcgggggccg
     3721 attcccggcg actcgcggac agatttcggg cgactcgcgg gccgatttcg ggcgactcgc
     3781 gggcggattt cgggcgactc gcgggttagg gggtgggggt gaggcccagg gcttgggctg
     3841 ccagttgggc caggtgcatg ggctcccgag tgccggcctg gcggagctgg gtgcgacagc
     3901 tgaagccgtc ggccaggact gccacgtcgg ggtcggccgc gcgcaccgcg ggcagcagca
     3961 cccgctccgc gcatgccatc gacacctcgt agtgcccctt ctcgaacccg aagttgccgg
     4021 ccagcccgca gcagccggag tcgagcacct cggcgtcgac gcccagcgct gtcagcaccg
     4081 cgcggtcgtc gtccgtgccg agctcggcgt gctggtgaca gtgcacctgc accagcgctc
     4141 gcctgccgcg ctccacgacc cggtcgcgca gctcgtcggc gtgctgcgcg aggacttcgg
     4201 cgaacgtccg cacaccactc gacaacgctg cggcgagcgg ctcgtcgggc agcaactcgg
     4261 gcccgtcgga gcgcagcgcg gcggtgcacg agggctccag ccccaccacg ggcacgccgg
     4321 ccgccaacca tggctcgatc gtgcgcaggc tgcggcgcag cacccggcgg gcggcgtcga
     4381 cctgaccggt ggacgtccag gtcagcccgc agcagacggc gcgaggtgga agttcgacgg
     4441 catagcccag cccctccagc accgccacgg ccgcgtccgc gatgccgggg tcgaagtggt
     4501 tggtgaacgt gtcgggccac agcagcaccc gcccccgcgg cgcgccggtc tccggcccgc
     4561 gagggcttcg gacggccgtg aacgggcggc gggcgatcgc gggaatcggc cgctccggcg
     4621 tgataccgcc tgccttcttc gcatacgccc cgagccggcc tcgggctgcc gcgttcagcg
     4681 cgtccaccat ccgcgcgggg agccgtccca ccagtcgcat ccaccgcggc aaggcgccca
     4741 tggagtagtg cgacgccggg cgtaccttcc gccggtagcg ctgcgccagg aactcggtct
     4801 tgtacgcggc catgtccacg cccaccgggc agtcgcgctt gcagcccttg caggacaggc
     4861 acaggtccag cgcctcggcc acctccggcg actgccagcc gcccgtgatg acgtcacctg
     4921 ccgccatctc gaacaggagg cgggcccggc cgcgcgtcga gtgcatctcc tcgccggtgg
     4981 cgcggtagct cgggcacatg acgccgccgt ccgcggtgac gcacttgccc acccccaggc
     5041 agcggcgggt ggcacgggtg aacgagccgc ggtcgtgggc gaacgcgagc tccggctcgt
     5101 ccggcagcgt cggcatgccg acgaacacgc ggaggtcctc gtccagcttc gcgggccgga
     5161 cgacccgacc ggggttcatc cggtcgtcgg gatcccagat ccccatgaac tcctcgaacg
     5221 ccgcgatgat ctccggcggg tacatccggg gcagcagctc cgcccgggcg gcgccgtcgc
     5281 cgtgctcgcc cgacagcgag ccgccgtgcg ccaccgcgag gtccgccgcg tcctccatga
     5341 agctgcggaa gtcggcgata ccggatcggg tgagcaggtc gaagtcgatc cgcacgtgca
     5401 ggcagccatc accgaagtgg ccgtagtagg cgccgcgccg ccggtgccgg gtgagcagcg
     5461 cgtcgaactc acgcaggtag gcaccgaacc gctccggcgg caccgcggcg tcctcccagc
     5521 cgggccacgc ctctccgccg tcgacgtatc gggtgacgat gccggcgccg tcctcgcgga
     5581 tgc
//
LOCUS       DASRSC010000094         3430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_357627,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000094 DASRSC010000000
VERSION     DASRSC010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357627"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..1500
                     /locus_tag="VFE39_04670"
     CDS             391..1500
                     /locus_tag="VFE39_04670"
                     /EC_number="4.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-lyase"
                     /protein_id="HET6257927.1"
                     /translation="MTGDGTRCVHGGHAGHVVGDPLHPGPVLSATFHLGGPDDPPAAD
                     FYGRAGNPTWRALEDAIGELDGGECVLFASGMAAIAAVLRLAGRDGALVLPSDGYYLA
                     RSLAHGELAPLGVEVREVPTVGECPAGVLDGAALVLLETPSNPGLDVADIAAVAAAAH
                     AAGALLAVDNTTATPLGQQPLALGADLAVASDTKALAGHGDVVLGHISVRNPALAQRL
                     REARARGGAVPGPMEAWLAHRGLGTLDLRLARQAANAAAVATAIASHPVVSDVRWPGL
                     PHDPAHAVAARQMRRWNGVLRFTLPCETAVGQFLAASRLVASATSFGGLRSSADRRER
                     WGDDVAPGLVRFSAGCEDEQDLVADVLAALDAVGA"
     gene            1664..1930
                     /locus_tag="VFE39_04675"
     CDS             1664..1930
                     /locus_tag="VFE39_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257928.1"
                     /translation="MTTPYTLAEQRAARARVDSGALAETADLLRQVARHDGHVDARRG
                     DVSASLAALVEAIGRGYREVPHEVAAVAMSVVAAVDRATGHRRS"
     gene            2156..2749
                     /locus_tag="VFE39_04680"
     CDS             2156..2749
                     /locus_tag="VFE39_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HET6257929.1"
                     /translation="MKHRQPDPNGADPEPGEVPGITPHPSVVPKGSRVLLLNATFEPL
                     AVVTAKRAVVLMLTGKAECIEAALDEVFHSENLTLPAPSVMRLSRYVRVPYRRAIPMT
                     RAGVLRRDGRRCGYCGRRADTIDHVIPRSRGGTHTWDNCVAACRTCNSRKADKMLVEL
                     GWNLDFVPRAPDRAAGGILVLAVEPPPAWEPWLGVAA"
     gene            2844..>3430
                     /locus_tag="VFE39_04685"
     CDS             2844..>3430
                     /locus_tag="VFE39_04685"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6257930.1"
                     /translation="MATVPVLVVGPHDMVTTSLVIALKANGFAAEKRDLNGPGPGFPR
                     PDRGGGIVVVNLDLPESVGLVAQAVRAGWHTLAMGKQNDRERAAAAVAAGAAAYVPRS
                     APVDVLVRAVMSIISGRPVMLTGERKAWLELHRTARAEVDTRRRHLDLLTDREFEVLR
                     RLERGQKAADIAVDAMVAMSTVRTHIRSILVKLEV"
BASE COUNT          469 a         1220 c         1260 g          481 t
ORIGIN      
        1 gagcgcagcg ggcgagaaga acatcccgtc cagctccagc cacagctcgc cgtggtggcc
       61 ggactccagg cggaagtggc ccgtacgggc cgacatcagg tcgagcacgg ccgcggagtg
      121 aatggtcatc gcggtttcga gggtagccgg gccgggcacc ggtcttgcgg gttcgatcac
      181 gaggggggcc gggttggcgc ctgccgttga cgtactaggt ctctggttcg ggatgagcca
      241 ggcggtcgtg caaccgggtg tggaagcgct gatggcgcac gttgcccgga ccctccgcga
      301 ccagttgcgg atgtactgag cgagcccggt cgccgtgctg agcagctcac cgggtgacgc
      361 ccccctgaga ctgccactta gggtgacgac gtgacggggg acgggacgcg ctgcgtgcac
      421 ggcggccacg cgggccatgt ggtgggggac cccctgcatc ccgggcccgt gctgtcggcg
      481 acgttccacc tcggcggccc cgacgacccg ccggcggcgg acttctacgg ccgcgccggc
      541 aacccgacgt ggcgcgcgct cgaggacgcc atcggcgagc tcgacggcgg cgagtgcgtg
      601 ctgttcgcgt cggggatggc tgcgatcgcg gcggtgctgc ggctcgcagg ccgcgacggc
      661 gcgctggtcc tgccgtcgga cggctactac ctcgcgcgtt ccctcgcgca cggcgagctg
      721 gcgccgctcg gcgtggaggt gcgcgaggtg ccgacggtgg gcgagtgccc cgccggggtg
      781 ctcgacggcg cggcgctggt gctgctggag acgccctcca accccgggct ggacgtggcc
      841 gacatcgccg ccgttgcagc cgccgcccac gccgccggtg ccctgctggc cgtggacaac
      901 acgacggcga ccccgctggg gcagcagccg ctggcgctcg gcgccgacct cgcggtcgcg
      961 agcgacacca aggctttggc cgggcacggc gacgtcgtgc tgggacacat cagcgtccgg
     1021 aacccggcgc tcgcgcagcg gctgcgcgag gcccgtgccc gcggcggtgc cgtgcccggg
     1081 ccgatggagg cctggctcgc ccaccgcggg ctgggcaccc tcgacctccg gctagcccgg
     1141 caggccgcca acgccgccgc ggtggcaacg gcgatcgcgt cgcaccccgt ggtgtccgac
     1201 gtccgctggc cggggctgcc gcacgatccc gcccacgccg tggccgcgcg gcagatgcga
     1261 cgctggaacg gcgtcctgcg gttcaccctg ccctgcgaga cggccgtggg gcagttcctg
     1321 gcggcgtcgc ggctcgtcgc gtcggccacc agcttcggcg gtctgcgcag cagcgccgac
     1381 cggcgggagc gctggggcga cgacgtcgcg cccgggctgg tgcgtttttc cgcaggctgc
     1441 gaggacgagc aggacctcgt cgcggacgtc ctcgccgccc tggacgccgt cggcgcctga
     1501 gcggcgcgca gttcttggat gaggtctccc acggccgtcc ctctgcgagg tgaccctgat
     1561 agaacaaatg ttcgaacgct gttagcgtct tctgggcggc ggccgggcgg gaagcgcccg
     1621 gcgagccgcg accagcacgt accagtcagg ggagctcgcc accatgacca cgccatacac
     1681 gctcgccgag cagcgggccg cccgcgcccg cgtcgacagc ggagcgctcg ccgagaccgc
     1741 tgatcttctt cggcaggtgg cgcgccacga cggccacgtg gacgcacggc ggggcgacgt
     1801 cagcgcgagc ttggccgcgc tcgtcgaggc aatcggacgc ggctaccgcg aggtgccgca
     1861 cgaggtggcc gccgtcgcga tgtccgtcgt cgccgccgtc gaccgggcca ccggccaccg
     1921 gcgctcgtga acgcgtgcga acgccggtgg gaccacccgg tgcccggcgc ctctgtgctg
     1981 ggcgttctgt tcgggtgcgc gcacaccgcg ggctcgtcgt tgcccacgcg ccgggtccca
     2041 gccgggagac ggcaccgtgt tggtcgacat cggcctcgtc cgatgtgaag aaggacccat
     2101 atcacacgtg cgtaatcccc tcaggttatg gtctgatctg cgaggaggtg gtcgcgtgaa
     2161 gcatcgacaa cccgacccca acggcgccga ccccgagcca ggggaggtac cggggatcac
     2221 gccgcacccg agcgtggtcc cgaaggggtc ccgggtcctg cttctcaacg cgacgttcga
     2281 gccgctcgct gtggtcacag cgaagcgtgc cgtcgtcctg atgctcactg gcaaggccga
     2341 atgtatcgag gccgccctgg atgaggtgtt ccactcggag aacctcaccc tcccggcccc
     2401 gtcggtgatg cggctgtccc gctacgtccg cgtgccctac cgccgcgcca tcccgatgac
     2461 gagagccggt gtcctgcgcc gcgacgggcg ccgctgcggc tactgcggca gacgcgccga
     2521 caccatcgac cacgtgatcc cccgcagccg cggcggcacg cacacgtggg acaactgcgt
     2581 cgcggcctgc cgcacctgca actcccgcaa ggccgacaag atgctcgtcg agctcggctg
     2641 gaacctcgac ttcgtgcccc gggcgcccga ccgcgcggcg ggcgggattc tggtgctggc
     2701 cgtggagccg cccccggcgt gggagccgtg gctcggagta gctgcataac acccgaaacg
     2761 tgatctaacg ccgctaacgc cgagtgacgt cccgacgacc cctttctcgt cacgaccttg
     2821 gaccagcagg ggggcgggga tcaatggcga ccgttcccgt gctggttgtc ggtccgcacg
     2881 acatggtcac cacgtccctc gtcatcgcgc tgaaggccaa cgggttcgcc gcagagaagc
     2941 gcgacctcaa cgggcctggt ccgggatttc cccggcccga caggggcggc gggatcgtcg
     3001 tggtgaacct cgacctcccc gagagcgtcg ggctggtggc ccaagccgtc agggcgggct
     3061 ggcacacgct cgccatgggc aagcagaacg atcgcgaacg ggcggctgca gccgtggccg
     3121 ccggggcagc ggcgtacgtc ccgcgcagtg ccccggtgga cgtgctggtc cgggcggtga
     3181 tgtccatcat ctccggtcga ccggtgatgt tgaccgggga gcggaaggcc tggctcgagc
     3241 tgcaccgaac ggcgcgggcc gaggtcgaca cgcgccggcg ccacctcgac ctcctgaccg
     3301 accgcgagtt cgaggtgctg cggcgcctgg aacgtggtca gaaggcggcg gacatcgccg
     3361 tcgacgccat ggtggcgatg agcacagtgc gcacccacat ccggtcgatc ctcgtgaagc
     3421 tcgaggtcaa
//
LOCUS       DASRSC010000095         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4452285,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000095 DASRSC010000000
VERSION     DASRSC010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4452285"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VFE39_04690"
     CDS             complement(<1..383)
                     /locus_tag="VFE39_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257931.1"
                     /translation="MDDQIADLSASFARSLCAEMRAARTVKLYGMSTRIFADWLVTQG
                     QPTTLDQFTRDNIRELLAHLAVDREASTVRIRFKGLHRFCGWLVAEGEIDVHPMAGMA
                     PPESAPKAGPVLQDDEHAALLRVCGG"
     gene            complement(435..1478)
                     /gene="ligD"
                     /locus_tag="VFE39_04695"
     CDS             complement(435..1478)
                     /gene="ligD"
                     /locus_tag="VFE39_04695"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET6257932.1"
                     /translation="MASKNAVLLTVPGPHGAREVRLTSADKVYFPERGITKGEVVEYY
                     RAVAEPLLGVLRERPTNLKRYPDGVDGEPFYSKRLPKGAPDYAETARVTFPSGRVAES
                     LCPTEPAVLLWAANMGTFDFHPWPVRRANPDRPDELRVDLDPQPGTDFADAVAVAAVL
                     HEVLDEAGLVGWPKTSGGRGIHVFARIRPEWDFIAVRHAVIAIARRVAERLPEKATVD
                     WWKEERGERVFLDFNQAARDRTVASAWSVRGRPRATVSMPLSWETLPDVEPEDFDVRT
                     VPGLFAERGDPHAGMDAAIGGIETALDWYAADERDRGLGEMPYPPEYPKMPGEPMRVQ
                     PSRARASTSENKS"
     gene            1510..1818
                     /locus_tag="VFE39_04700"
     CDS             1510..1818
                     /locus_tag="VFE39_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257933.1"
                     /translation="MPELHEPDRSSARSRQFDDELVGLDPDDPEAQAFAAHLDRMQCD
                     RPAYTVEGYLDGVRDFADSANRAEGERRWAAVLVVLLLLAVAAYLVGDALLFVLVTWL
                     "
     gene            complement(1831..2164)
                     /locus_tag="VFE39_04705"
                     /pseudo
     CDS             complement(1831..2164)
                     /locus_tag="VFE39_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642691.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding response regulator"
BASE COUNT          291 a          775 c          760 g          338 t
ORIGIN      
        1 cctccgcaca ccctcagcag cgccgcgtgt tcgtcgtcct gtagtaccgg accggctttg
       61 ggggccgact cgggtggcgc catccctgcc atcgggtgga cgtcgatctc gccctcggcg
      121 accagccacc cacagaaacg atgtagaccc ttgaaccgga tccggacggt ggaggcctcg
      181 cggtcgaccg ccagatgcgc gagcaactcg cggatgttgt cgcgggtgaa ctggtccagg
      241 gtggtgggtt ggccctgcgt gacgagccag tcggcgaaaa tccgcgtcga catcccgtag
      301 agcttgacgg tgcgcgcggc cctcatctcg gcacagaggc tccgcgcgaa gctcgcagag
      361 aggtccgcga tctgatcgtc caccggggtc ggttatcctg cgagctttgc tctataccat
      421 cggcgcggac tgctctacga cttgttttcg ctggtagagg ccctagcgcg ggacggctgg
      481 acccgcattg gctcgcccgg catcttgggg tactcgggcg ggtacggcat ctcccccagc
      541 ccgcggtccc gctcgtcggc ggcgtaccag tccagtgccg tctcgatccc gccgattgcc
      601 gcgtccatcc ccgcgtgcgg gtcaccccgc tcggcgaaga gcccgggcac cgtccgcacg
      661 tcgaagtcct ccggctcgac gtccggcagc gtctcccagc tgagcggcat cgacacggtg
      721 gcgcgcgggc gcccccgcac cgaccaggcc gatgcaaccg tccggtccct tgccgcctgg
      781 ttgaagtcga ggaacacgcg ttcgccgcgc tcctccttcc accagtcgac ggtggctttc
      841 tcgggcaggc gctcggccac ccggcgggcg atcgcgatca ccgcgtgccg cacggcgatg
      901 aagtcccact ccggccggat ccgggcgaac acgtggatgc cccgaccgcc cgaggtcttg
      961 ggccagccga cgagcccggc ctcgtcgagc acctcgtgca gcacggcggc caccgccacg
     1021 gcgtcggcga agtcggtacc cggctgcggg tcgaggtcga cgcgcagctc gtcggggcgg
     1081 tcgggattgg cccggcggac gggccacggg tggaagtcga acgtgcccat gtttgctgcc
     1141 cacagcagca ccgcgggctc ggtggggcag agcgactcgg cgacccggcc gctcgggaag
     1201 gtcacccgcg ccgtctccgc gtagtccggg gcccccttgg gcaggcgctt ggagtagaac
     1261 ggctcaccgt cgaccccgtc cggatagcgc ttgaggttgg tggggcgctc gcgcagcacc
     1321 ccgagcagcg gctctgccac ggcgcggtag tactccacga cctcgccctt ggtgatcccc
     1381 cgctccggga agtacacctt gtccgcgctg gtcaaccgca cctcacgggc gccgtgcggc
     1441 ccggggacgg tgagcagcac cgcgttcttg ctcgccacgc tggcgcacgc taccggctac
     1501 cctcgttgga tgcccgaact ccacgagccg gaccggtcat cggcccggtc ccggcagttc
     1561 gacgacgagc tggtcgggtt ggatcccgac gacccggagg ctcaggcgtt cgccgcccac
     1621 ctcgatcgca tgcagtgcga tcggcccgcc tacacggtgg agggctacct cgacggtgtc
     1681 cgcgacttcg ccgactccgc caaccgggcc gagggcgagc ggcgctgggc ggccgtgctg
     1741 gtggtcctgc tgttgctcgc cgtggcggcc tacctggtgg gggacgcgct cctgttcgtg
     1801 ctggtcacct ggctgtagcg ccaggcacta ctcccggttg gccaggccgc gggccaccag
     1861 ctccaagact tcccgctcgc gcgtggtgag acgaagcagc cgctctttcg cggcggggtc
     1921 gatgcgcccg cgcctggtga cgtcggcgat cagccgcctg gtcaccgacg gggcgagcag
     1981 cgcgtcgccg gcggcgacca cccggaccgc ggcagcgagc tcagcggtgg gggcgtcttg
     2041 agcaggaagc cgctcgcgcg gagggcgtcg tattagtcgt cgatgtcgaa cgtggtcagc
     2101 acgagcaccc gggtgggcga ctcgacgggc tcggcgagca gctcacgggt ggcagtgagc
     2161 ccat
//
LOCUS       DASRSC010000096         5418 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4943362,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000096 DASRSC010000000
VERSION     DASRSC010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5418
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4943362"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..1573
                     /locus_tag="VFE39_04710"
     CDS             251..1573
                     /locus_tag="VFE39_04710"
                     /inference="COORDINATES: protein motif:HMM:NF013918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET6257934.1"
                     /translation="MDQRLLRAAWLSAFGLIVGAPSLAWLTIAPVEPLWDHLSVVTGL
                     LALSALVCAAVLPSRLRSLNRAFGIESVIDVHRFLGIVAAALVFVHLACVIAADPANV
                     ALLDFETAPKRAQAATGATVALAVLAVLAVLRGRTRLTYELWRWLHVVLAVIVLVLSA
                     LHVWWLDHLVAEPTLGTLFGLLAALVVGTFGYRWAWRPMFDASTEFLVREVRRENATV
                     STLVLEPRRRHGRVNTWAFAPGQFAWIRLDRSVTAEEHPFTIASSAHLDTTEFTIRHA
                     GDFTRALRRLPPGAPVWVDGPHGAFTNDVGAGAGFVMIAGGVGVTPMMSMLRTAAHRG
                     DPRPYRLVVVAGSPEDLLFRDELAELRFQIDLVVTEVLRRPLDGWYGHTGDINAGLLS
                     MVLADAGHLDEVDYFICGPSSLVTDVLSALDVLGVAPARVHTEQFDFV"
     gene            1609..2733
                     /locus_tag="VFE39_04715"
     CDS             1609..2733
                     /locus_tag="VFE39_04715"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HET6257935.1"
                     /translation="MLRRAVVVAVILAVSATVYQSWASGSPGTGGWVQTQWGPLGPAD
                     RDLLVKVRLAGLWEQPTGQQAEQQASSPEVKNAGLDISSEHGSLDEEVRKVANQLGVL
                     LPSSPTAQQVAWMNEISQQTGSAYDRMFVQRLRAAHGAVLPVIAQVRATTQNELMRQF
                     ADTAEQYVSRHIKYLEDTGLVDYAALPQSSPGLLSGGTGPGDLVVPILVFVAALLAAI
                     AIFAALRGRNAARRNRVTVPQRAPATHLPELTAIAAIPAPRSTSRADPDSGSYSSVSD
                     TRSPRWTSSDSGPLPTVSDSGSYHAATDTGSYQAATDTGPYRSVSDTGFHPVSDTGSH
                     PVSDTGSHRTVSDTGSHRAVSDTGSHRLTGRPRSRHSIRR"
     gene            2819..3778
                     /locus_tag="VFE39_04720"
     CDS             2819..3778
                     /locus_tag="VFE39_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257936.1"
                     /translation="MSPSSTERRRSTARKLAISAAALVVTVLPTACGSAAYADSLSEG
                     KKASTAPSVGGHSSHGSHDESASTRAGTVVPAGNTDEPSGGNKEADSQGEKSNGSGDS
                     NGSGDSNGSGDSNGSGDQKGSGDGNGSDQQQAPVNNGLDVLGTDCSNSDLPPHDGFQL
                     APRCVSTSFGEVAAEDKSPSLLITDSPRKVDVNEKFTIKVSTRNLVRDRFLGAAAGGY
                     YLESSFLNKDGLQRGHFHTACRMLDSTDVAPDAAPAPEFFLATQDNGGGAEPDTVTIT
                     VTGMPTAGTAQCAVWAGDGSHRMPMMQRADQTPAFDAVRIDVK"
     gene            complement(3799..4368)
                     /gene="pyrE"
                     /locus_tag="VFE39_04725"
     CDS             complement(3799..4368)
                     /gene="pyrE"
                     /locus_tag="VFE39_04725"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET6257937.1"
                     /translation="MNADTERDELAALVRDLGVVHGRVTLSSGAEADYYIDLRRVTLE
                     HRAAPLVGRLLRVLTADWDYSAVGGLTLGADPVADAVLHAAAAERAALPDTPPVDAFV
                     VRKDTKQHGMQRLIEGPDIAGRHVLVVEDTSTTGASVLTAVRAVRAAGATVAGVATVV
                     DRDTGAREAIEAEDAPYRALLGLADLGLT"
     gene            complement(4401..5174)
                     /locus_tag="VFE39_04730"
     CDS             complement(4401..5174)
                     /locus_tag="VFE39_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6257938.1"
                     /translation="MPTALITGATAGIGAAFASRLARDGRDLVLVARDKDRLEAAAAR
                     YRAAGVAVEVLPADLSVTEERDRVAARLSDVDVAPVDLLINNAGFVLSQGLIKADAAA
                     LQRQHDVNVTSVLQLTTAALPSMIGRGRGAVINVSSVSAFLPGRDSAYTADKAWVTAY
                     SEAVAGALAGTGVRVIALCPGFVRTEFHERAGLDVGRRRGPLWLAADRVVEECLADLA
                     RGKVVSVPSPQYKAIVALLDVLPRRLLWRLMAAAERDRT"
     gene            complement(5239..>5418)
                     /locus_tag="VFE39_04735"
     CDS             complement(5239..>5418)
                     /locus_tag="VFE39_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257939.1"
                     /translation="EASDRVGDDVELLWAEESWVLATAPLGSSAERVQELLADLADVA
                     TVLEQSLGVGPGVDR"
BASE COUNT          777 a         2040 c         1813 g          788 t
ORIGIN      
        1 cggcggcccc gtccgcccgg ggccctgtcc tcgtacggtc cgaggctgtc ggcgagtggc
       61 cgggtacaag ttgcgcgtgc ggacctgtgc tcgccgcggc aagatcctga ggctgttacg
      121 tggcattcgg cctaattaac cggacaagtg gggcaatctc agaccgctcg tcgcgtccgc
      181 agccacttct ggcccacagg gctctcagct tcgcaacctg tacgggaggt cgcctcctac
      241 ggtccgggcc atggatcagc ggttgttgcg cgcggcgtgg ctgagtgctt tcggcctgat
      301 cgtcggcgca ccctcgctcg cgtggctcac gatcgctccc gtcgagccgc tctgggacca
      361 cctctcggtc gtcaccgggt tgctggcgct ctccgcgctc gtctgcgcag cggtgctgcc
      421 gtcccgactc cgctccctga accgtgcctt cggcatcgag agcgtgatcg acgtccaccg
      481 cttcctcggt atcgtcgcgg cggcgctggt gttcgtccat ctcgcctgcg taatcgccgc
      541 cgacccggcc aacgtcgcgc tgctggactt cgagaccgct ccgaagcggg cacaggccgc
      601 caccggcgcg accgtcgcgc tcgccgtgct cgccgtgctc gccgtcctgc gcgggcggac
      661 acggctcacc tatgagctct ggcggtggct ccacgtggtc ctcgcggtca tcgtgctcgt
      721 gctgtcggcg ctgcacgtgt ggtggctcga ccaccttgtg gccgaaccca cgctgggcac
      781 gctcttcggg ctgctcgcgg ccctcgtggt ggggaccttc ggataccgct gggcctggcg
      841 gccgatgttc gatgcgtcga ccgagttcct ggtccgcgag gtcaggcggg agaacgcgac
      901 ggtgagcacg ctcgtgctgg agccgcgccg ccgacacggt cgggtgaaca cgtgggcctt
      961 cgcccccggt cagttcgcct ggatccggct ggacaggtcg gtgaccgccg aggagcaccc
     1021 gttcaccatc gcctccagcg cacacctgga caccaccgag ttcacgatcc gccacgccgg
     1081 cgacttcacg cgcgcgctgc gccgcctccc gccgggcgcc ccggtttggg tggacggtcc
     1141 gcacggtgct ttcaccaacg atgtcggcgc gggggccggc ttcgtcatga tcgccggcgg
     1201 cgtcggggtg acgccgatga tgagcatgct gcgcacggcc gcgcaccgcg gggatccgcg
     1261 gccgtaccgg ctggtcgtcg tggccgggtc gccggaggac ctgctgttcc gcgacgagct
     1321 cgcggagctg cggttccaga tcgacctcgt ggtcaccgag gtcctgcgcc ggccgctcga
     1381 cggctggtac gggcacaccg gcgacatcaa tgcgggcctg ctctcgatgg tgctcgcgga
     1441 cgcagggcac ctcgacgagg tcgactactt catctgtggc ccgtcatcgc tggtcacgga
     1501 tgtcctgagc gccctcgacg tcctcggtgt ggcaccggcc cgcgtgcaca ccgaacagtt
     1561 cgacttcgtg tgaacaggaa ggcaccaatg ctccgcagta tcccgcgatt gctccgacgc
     1621 gccgtggtcg tcgccgtgat cctcgccgtg tccgcgacgg tgtaccagtc gtgggcctcg
     1681 gggtcccccg gcacgggtgg ctgggttcag acccagtggg gcccgctcgg ccctgccgac
     1741 cgtgacctgc tcgtcaaggt ccggctggcc ggtctctggg agcagccgac gggccagcag
     1801 gcagagcagc aggcgagttc acccgaggtg aagaatgccg ggctggacat ctccagcgag
     1861 cacggctcgc tcgacgagga ggtgcgcaag gtcgccaacc aactcggcgt cctgctgccc
     1921 agcagtccca ccgcccagca ggtggcctgg atgaacgaga tctcgcagca gacgggctcc
     1981 gcgtacgacc ggatgttcgt ccagcggctg cgtgcggcgc atggcgcggt cctgcccgtc
     2041 atcgcgcagg tgcgggccac cactcaaaac gagctgatgc ggcagttcgc cgacacggcc
     2101 gagcagtacg tcagccggca catcaagtac ctcgaggaca cggggctggt ggactacgcg
     2161 gcgctcccgc agtcgtcacc gggcctgctg tcgggtggta ccgggccggg cgacctcgtc
     2221 gtgccgatcc tggtgttcgt cgccgcgctc ctcgccgcca tcgccatatt cgcggccctg
     2281 cgcgggcgga acgccgcgcg ccgcaaccgg gtcaccgtcc cccagcgcgc cccggccacg
     2341 catctccccg agctcaccgc catcgcggcg atccccgctc cccggtcgac atcgcgcgcc
     2401 gaccccgaca gcggttccta cagctctgtc tccgacacga ggtccccccg ttggacgtcg
     2461 tccgacagcg gcccgctccc cacagtctcc gactcgggct cctaccacgc cgccaccgac
     2521 acgggctcct accaggccgc caccgacacg gggccctacc gcagcgtctc cgacaccggg
     2581 ttccacccgg tctccgacac cggctcgcac ccggtctccg acaccggctc gcaccggacg
     2641 gtctccgaca ccggctccca ccgggccgtc tcggacaccg gatcccaccg cctcacgggc
     2701 cgcccccgtt cccgccattc catccggcgg tagctctccg ctccccgcat ctccgcctga
     2761 ccaccggtag tgccacctgg ccctcccgtt cccgctcccc gaccccgaac ggagcagcat
     2821 gtccccctcc agtaccgaac gacgccggag tacggctcgt aagctggcga tctccgccgc
     2881 cgccctggtg gtgaccgtcc tccccaccgc ctgcggctcc gccgcgtacg ccgattccct
     2941 gagcgagggg aagaaggcgt ccacagcacc ctcggtgggc ggccacagca gccacggcag
     3001 ccacgacgag agcgcctcga cgcgtgcggg caccgtcgtt cctgctggca acacagacga
     3061 accgtccggg gggaacaagg aggcagactc gcagggcgag aagtcgaacg gatccggcga
     3121 ctcgaacgga tcgggagact cgaacggatc gggagactcg aacggatccg gcgaccagaa
     3181 ggggtccggc gacgggaacg ggtccgacca gcagcaggcg ccggtgaaca acggactcga
     3241 cgtcctcggc acggactgct ccaacagcga cctcccgccc cacgacggct ttcagctcgc
     3301 gccgcgctgc gtgagcacct cgttcggcga ggtggccgcc gaggacaaga gcccgtcgct
     3361 gctcatcacg gactcgccgc gcaaggtcga cgtcaacgag aagttcacga tcaaggtgag
     3421 cacgcgcaac ctcgtgcgcg accggttcct cggtgccgcc gccggcggtt actacctcga
     3481 gagctccttc ctcaacaagg acggcctcca gcgcgggcac ttccacaccg cctgccggat
     3541 gctcgactcc acggacgtcg cgcccgacgc ggcacccgca ccggagttct tcctggccac
     3601 gcaggacaac ggcggcggtg cggagcccga caccgtgacc atcaccgtca ccggcatgcc
     3661 gaccgcagga accgctcagt gcgcggtgtg ggcaggcgac ggctcccacc gcatgccgat
     3721 gatgcagcgg gcggaccaga ccccggcctt cgacgcggtt cgcatcgacg tcaagtaacc
     3781 cggcggcgct ctccggcctc aggtgaggcc gaggtcggcc aatccgagca acgcccggta
     3841 cggggcgtcc tcggcctcga tcgcctcgcg ggcgccggtg tcgcgatcca ccactgtggc
     3901 cacaccggcg accgtggccc ccgcggcgcg caccgcccgg acggcggtga gcacggacgc
     3961 accggtcgtc gaggtgtcct cgaccacgag gacgtggcgg cccgcgatgt ccggcccttc
     4021 gatgagccgc tgcatgccgt gctgcttggt gtccttccgc acgacgaacg catcgaccgg
     4081 aggggtgtcg ggcagggccg cgcgttctgc cgccgcggcg tgcaggaccg cgtcggccac
     4141 cgggtcggcg ccgagggtca ggcccccgac ggcggagtag tcccagtctg cggtgagcac
     4201 ccgcagcagc ctgccgacca gcggcgcggc gcggtgttcg agggtgacgc ggcgtaggtc
     4261 gatgtagtag tcggcctcgg cgcccgacga cagcgtgacg cgcccgtgca ccacacccag
     4321 gtcgcgaacc agcgccgcga gctcatcgcg ctcggtgtca gcgttcatgc cgtcatcctc
     4381 acaaagcggt tgccgggcgt tcatgtgcgg tcccgctcgg cggcggccat gagccgccag
     4441 agcagccgtc gcggcagcac gtcaagcagg gcgacgatcg ccttgtactg cgggctcggc
     4501 accgacacca cctttcccct cgccaggtcg gccaggcact cctcgacgac gcggtccgcg
     4561 gcgagccaga gcggtcccct ccgcctgccg acgtcgagac cggcacgctc gtggaactcg
     4621 gtgcggacga aaccgggaca gagcgcgatc acccgcacgc cggtgccggc gagcgcgccc
     4681 gccacggcct cggagtaggc ggtgacccac gccttgtcgg cggtgtaggc cgagtcgcgg
     4741 cccgggagga aggcgctcac gctcgatacg ttgatcaccg caccacgccc cctgccgatc
     4801 atgctcggca gtgcggccgt ggtcagttgc agcaccgacg tcacgttgac gtcgtgctgg
     4861 cgctgcaacg cagcggcatc cgccttgatg agaccctggg aaagcacgaa gccggcgttg
     4921 ttgatcagga ggtcgaccgg tgcgacgtcc acgtcggaca accgagcggc cacccggtcc
     4981 cgctcctccg tgacggagag gtcggcgggc agcacctcga cggcgacacc ggcggcccga
     5041 taccgcgccg cggcggcctc cagccgatcc ttgtcacgag cgacgagcac gaggtcccgg
     5101 ccatcgcgtg cgagtcggga cgcgaacgcg gccccgatgc ctgcggtggc tcctgtgatc
     5161 agcgcggtgg gcacggcgcg agcctagtga cgaacatccg cccgccacct cgcggccgga
     5221 cgggcgcgtc cgagaaggtc agcggtccac gcccggcccc acgccgagcg actgctcgag
     5281 cacggtggcc acgtccgcga ggtcggcgag cagctcctgc acccgctccg cgctggagcc
     5341 gagcggcgcc gtcgcgagca cccaggactc ctccgcccac agcagctcca cgtcgtcccc
     5401 gacccggtcg ctcgcctc
//
LOCUS       DASRSC010000097         4926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3632426,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000097 DASRSC010000000
VERSION     DASRSC010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3632426"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VFE39_04740"
     CDS             complement(<1..1007)
                     /locus_tag="VFE39_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6257940.1"
                     /translation="MTSYTDFTIGAPVADTAAPAGPVEERWERRRFGVKLVNPANKRK
                     MKIIVVGTGLAGGSAAASLAELGYQVSSFCYQDSPRRAHSIAAQGGINAAKNYRNDGD
                     SVHRLFYDTVKGGDFRSRESNVHRLAEISLQIIDQCVAQGVPFAREYGGLLDTRSFGG
                     AQVSRTFYARGQTGQQLLLGAYQALERQIDAGGVMMYPRHEMLDLIVVDGRARGIVAR
                     DLVTGETRSHLADAVVLASGGYGNVFYLSTNAKGCNVTASWRAHRRGALFANPCYTQI
                     HPTCIPVTGDYQSKLTLMSESLRNDGRVWVPRAGGDKRPPAEVPEAERDYFLERKYPS
                     FG"
     gene            complement(1010..1720)
                     /locus_tag="VFE39_04745"
     CDS             complement(1010..1720)
                     /locus_tag="VFE39_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HET6257941.1"
                     /translation="MVTLGERPAARVRHTPRPSSVQLKIVMAVSGALALLYLVAHMIG
                     NLKIFLGADAINTYAAWLRTVGEPALPAQTLLWIVRAVLTVAIVAHIVSAVILTRRAH
                     RARPIKYAHRPPVQGSYAARTMRWGGVIIVLFIVYHLLDLTTGTLNPNGVSGDVHANV
                     VADFAPHRWYVTLFYAIAVVAVGFHVRHGLWSGLQTLGRSSAAAQNTLKGVALVVAVA
                     LTVGFLSVPFAVLTGLVR"
     gene            1797..2741
                     /locus_tag="VFE39_04750"
     CDS             1797..2741
                     /locus_tag="VFE39_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET6257942.1"
                     /translation="MTLQQLAYFVAVADVRSFTRAAESLGVAQPTLSRQLRALEDELG
                     APLVDRGGRDGPVLTPAGEAVLPLARRMLADSESARTAVAEIVGLRSGRVRVGATPSL
                     CIGVLADVLRVFHDKYPDIRLELVEGGSQPLVRSLVRGELDVALVIVPPAGIDPALHT
                     TPVLRERLSVASPTSDRSPSSRGSMTVRELARRPLVVPRQGYDVREATLQAYADAGET
                     PRFAVEGGEMDAVLRLVEAGTGVAVVPDLVFAGRPRLRRTMLTPPLYRTVALARRADL
                     TPTHAVRAFRDTLLSFLSVLSAGDGFPGDVQVLRRPDT"
     gene            2780..3508
                     /locus_tag="VFE39_04755"
     CDS             2780..3508
                     /locus_tag="VFE39_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HET6257943.1"
                     /translation="MADADIHFYFDPVCPFAWMTSKWVRMVQAERDYRVDWRFISLRL
                     LNSHVDYDAHFPPEYKAGHTAGLRLLRVAARTRSELGRDAVGPLYAALGAQIFDRDGA
                     RNDAAERERRGTRAFVEPVLAGIGLPTTLADALDDPTWDPEIQSETDEALTLTGKDVG
                     TPILHFEPPTGVAFFGPVISRLPSRDQAAELWDHVIALARFPGFSELKRSLREQPQLR
                     SFGVREDAVGVQEDWHGGSRRQKK"
     gene            complement(3580..4800)
                     /locus_tag="VFE39_04760"
     CDS             complement(3580..4800)
                     /locus_tag="VFE39_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HET6257944.1"
                     /translation="MAPGREHAAPEQNAVRVVRLDGIGTGDGVNAPVARLDATDSSAP
                     AGGPAADLDRLDCLAAEAAPVASFPLDDGRGAAAVEVSASGGARSDEGAAPVALFAAE
                     ELTAARGGAPVAELGTAARNGPARRRGRSPSGGDARADRAAPSPDDQGVGHTGDRAAD
                     RAGDRAGRRTARRAAGRRAEAAANEGVDRRADQAAAGGTGDRSAEWTANRATGRRAAR
                     AADRAEGRSGGDSGHDTDPAARAREICLRLLTDRARTRQELAQALHRKGIPDTVAGSV
                     LERFDEVGLIDDAAFAGQWVRSRHAHRGLARRAIAVELRRKGVADEVAGEALAEVDGE
                     SEERRARQLVDRKLRSLTVASTEQRAAAGRRLVGMLARKGYGAGVAYRVVREALAELG
                     ADVEELDAAEPPDD"
     gene            complement(4803..>4926)
                     /locus_tag="VFE39_04765"
     CDS             complement(4803..>4926)
                     /locus_tag="VFE39_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA recombination/repair protein RecA"
                     /protein_id="HET6257945.1"
                     /translation="NFLRENVDTANEIEKKIKEKLGIGAQLDAEAEPLPAPVDF"
BASE COUNT          651 a         1755 c         1796 g          724 t
ORIGIN      
        1 ccgaaactcg gatacttccg ctccaggaag tagtcgcgct cggcctcggg gacctccgcc
       61 ggcgggcgct tgtcgcctcc cgccctgggc acccacaccc ggccgtcgtt gcgcagcgac
      121 tccgacatga gggtgagctt cgactggtag tcgccggtga cggggatgca cgtggggtgg
      181 atctgcgtgt agcacgggtt ggcgaacaga gccccccgcc ggtgtgcccg ccagctcgcc
      241 gtgacgttgc agcccttggc gttggtggag aggtagaaga cgttgccgta gccgccggag
      301 gcgagcacca cggcgtcggc gagatggctg cgggtctccc ccgtcaccag gtcgcgggcg
      361 acgatgccgc gggcgcggcc gtcgaccacg atgaggtcga gcatctcgtg gcgcgggtac
      421 atcatcacgc cgcccgcgtc gatctggcgc tccagtgcct ggtaggcgcc gagcagcagc
      481 tgctggcccg tctgcccgcg ggcgtagaag gtgcgggaga cctgcgcacc gccgaaggag
      541 cgggtgtcga ggagcccgcc gtactcacgg gcgaacggca caccctgcgc cacgcactga
      601 tcgatgatct ggaggctgat ctcggccagc cggtggacgt tggactcccg ggaccggaag
      661 tcgccgccct tgacggtgtc gtagaacagc cggtgcacgc tgtcgccgtc gttgcggtag
      721 ttcttcgcgg cgttgatgcc gccctgtgcg gcgatgctgt gggcgcggcg tgggctgtcc
      781 tggtagcaga acgagctgac ctggtagccc agctccgcca ggctcgcggc ggcgctcccg
      841 ccggcgaggc cggtgccgac cacgatgatc ttcatcttgc gcttgttggc cgggttcacc
      901 agcttgacgc cgaagcgcct gcgctcccag cgctcctcga ccgggcccgc gggcgcggcg
      961 gtgtcggcca cgggggcacc gatggtgaag tcggtgtagc tggtcatggt catcgcacca
     1021 atccggtcag gaccgcgaac gggacggaca ggaagccgac ggtgagcgcc accgcgacca
     1081 cgagggcgac tcccttgagg gtgttctggg cggcggcact ggagcggccg agcgtctgca
     1141 ggccgctcca caggccgtgc cggacgtgga agcccaccgc cacgaccgcg atcgcgtaga
     1201 agagcgtgac gtaccaccgg tgcggcgcga agtcggccac cacgttcgcg tggacgtcac
     1261 cggagacgcc gttggggttc agcgtgccgg tggtgaggtc gaggaggtgg tagacgatga
     1321 acagcacgat gatcaccccg ccccagcgca tcgtgcgggc tgcgtagctg ccctgcacgg
     1381 gcggccggtg ggcatacttg atcggccggg cccggtgggc gcggcgcgtg aggatcacgg
     1441 ccgacacgat gtgggccacg atcgcgacgg tgaggaccgc gcgcacgatc cacagcagcg
     1501 tctgcgccgg aagggcgggc tccccgacgg tgcgcaacca cgcagcgtag gtgttgatgg
     1561 catcggcgcc gaggaagatc ttcaggttgc cgatcatgtg ggccaccagg tagagcagcg
     1621 cgagagcgcc ggacaccgcc atcacgatct tcagctggac cgacgacggt cgcggcgtgt
     1681 gccggacgcg agccgccggg cgctctccca gagtcaccat gcgccaaggg tgcgtaccgg
     1741 ccccgcgaac gtccaatgca tcgaagcctt gttaccgatg cacgtacgct ataggcgtga
     1801 cgcttcagca gctcgcgtac tttgtggccg tggccgacgt gcgcagcttc actcgggcgg
     1861 ccgagtccct cggcgtggcg cagcccacgc tgtcccgcca gctgagggcg ctggaggacg
     1921 agctcggtgc tccgctcgtg gaccgcggag gccgggacgg acccgtgctg acaccggccg
     1981 gcgaggccgt actgccgctc gcccggcgca tgctcgccga ctcggagagc gctcgcaccg
     2041 cggtcgccga gatcgtcggg ctgcgcagcg gccgggtacg cgtcggggcg acgccgtcgc
     2101 tctgcatcgg cgtgctggcc gacgtgctgc gggtgttcca cgacaagtat cccgacatcc
     2161 ggctggagct ggtggagggc gggtcgcagc cgctggtgcg gtcactggtg cgcggtgagc
     2221 tcgacgtcgc gctggtgatc gtgccgccgg cggggatcga cccggccctg cacacgacgc
     2281 cggtgctgcg ggagcgcctg tcggtggcct caccgacctc cgaccggtcg ccgtcgtcgc
     2341 ggggctcgat gacggtgcgg gagctggccc ggcggccgtt ggtggtgcca cggcagggct
     2401 acgacgtgcg ggaggcgacc ctgcaggcct atgccgatgc cggggagacg ccgcggttcg
     2461 cggtggaggg cggggagatg gacgccgtgc tcaggctcgt ggaggcgggc accggcgtgg
     2521 cggtggtgcc ggacctggtg ttcgcgggca ggccgcggct gcgccgcacc atgctcacgc
     2581 cgccgctcta ccgcaccgtc gcgctggccc ggcgcgccga cctcaccccg actcacgccg
     2641 tgcgtgcgtt ccgcgacacc ctgctgtcct tcctctccgt cctgtcggca ggcgacgggt
     2701 tcccgggaga cgtgcaggtc ctgcgccgtc cagacacgtg aggcagcgcc gcccggaacg
     2761 gcgggttagc ctcggtgagg tggccgatgc ggacatccac ttctacttcg atcccgtctg
     2821 cccgttcgcc tggatgacca gcaaatgggt gcgcatggtc caggccgagc gggactaccg
     2881 ggtggactgg cgattcatct cactgcgact gctcaactcc catgtcgact acgacgcgca
     2941 cttcccgccg gagtacaagg caggccacac agcgggcctg cggttgctgc gcgtggcggc
     3001 gcgcacccga tccgagctcg ggcgcgacgc tgtcggcccg ctctacgccg cgctgggcgc
     3061 gcagatcttc gaccgcgacg gggcgcggaa cgacgcagcc gaacgtgagc gccgcggaac
     3121 cagggcgttc gtcgaaccgg tgctcgccgg gatcggctta ccgaccactc tggccgacgc
     3181 cctcgacgac ccgacctggg accccgagat ccagagcgag accgacgaag cgctcaccct
     3241 gacgggcaag gacgtcggca ccccgatcct gcatttcgag ccaccgaccg gcgtcgcgtt
     3301 cttcggtccg gtgatcagca ggctcccgtc ccgcgaccag gccgccgagc tgtgggacca
     3361 cgtcattgcc ctggctcgct ttcccgggtt ctccgagctg aagcggagcc tgcgcgagca
     3421 gccccagttg cgttccttcg gcgttcgcga ggatgcggtg ggcgtgcagg aggactggca
     3481 cggcggcagt cgccggcaga agaagtagcg gcgagggcgc actctgcgcg atccgccagg
     3541 tcgaccagcg cgcagatccc ggggtgccga cagtgcggct cagtcgtcgg gtggttccgc
     3601 cgcgtcgagc tcctcgacgt cggcgcccag ctcggccagg gcctcccgca ccacccggta
     3661 ggccacgccg gcgccgtagc ccttgcgggc cagcatcccg accagccggc gcccggcggc
     3721 cgcgcgctgc tcggtggagg cgaccgtgag ggagcggagc ttgcggtcga cgagctggcg
     3781 ggcccgccgc tcctcggact caccgtccac ctcggccagc gcctccccgg caacctcgtc
     3841 ggcgacgccc ttgcggcgca gctcgaccgc gatcgcacgc cgggcgaggc cgcggtgcgc
     3901 gtgccgtgag cggacccact ggcccgcgaa ggcggcgtcg tcgatcaggc cgacctcgtc
     3961 gaaccgctcg agcaccgagc cggccacggt gtcggggatg cccttgcggt gcaacgcctg
     4021 cgcaagctcc tgccgcgtgc gagcgcggtc ggtgagcaac cgcaggcaga tctcgcgcgc
     4081 cctggcggcg ggatcggtgt cgtgccccga gtcgccgccc gaccgacctt cggccctgtc
     4141 tgctgctcgg gctgccctcc ggcccgttgc ccggttcgcc gtccactccg ctgaccgatc
     4201 cccggtccca cctgctgctg cctggtctgc cctccggtca actccctcgt tggctgccgc
     4261 ctccgctctc cggcccgctg cccggcgtgc tgtgcggcgt cctgcccgat cgccggcacg
     4321 gtccgctgcc ctgtctccgg tgtgccctac cccctggtcg tcgggcgacg gagcggcccg
     4381 gtcggcacgg gcgtcacccc cgctcggcga tcgcccccgg cgccgggcag ggccgttccg
     4441 agcagcggtg cccagctcgg ccacaggtgc gccaccgcgc gcggccgtga gctcctcggc
     4501 ggcgaacaaa gcaaccggcg ccgcgccctc gtcactgcgc gcgccaccgg acgcggagac
     4561 ctcgacggcc gctgcgcccc gcccgtcgtc cagcgggaaa gaggcgaccg gagctgcctc
     4621 ggcggcgagg cagtcgaggc ggtcgaggtc agcggccgga ccaccggccg gggcggacga
     4681 atccgtggcg tccagccggg cgacgggagc attcacaccg tcgccggtgc cgattccgtc
     4741 gagccggacg acgcggaccg cattctgctc cggggcggcg tgctcccggc ccggcgccat
     4801 gctcagaagt cgacaggtgc cggcagcggc tcggcctcgg cgtcgagctg ggccccgatg
     4861 ccgagcttct ccttgatctt cttctcgatc tcgttggccg tgtcgacgtt ctcgcgcagg
     4921 aagttc
//
LOCUS       DASRSC010000098         6826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4288816,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000098 DASRSC010000000
VERSION     DASRSC010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4288816"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="VFE39_04770"
     CDS             <1..1249
                     /locus_tag="VFE39_04770"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6257946.1"
                     /translation="WTRLFAQQPEIRAYLEDIADRYGLRARIRFRSTVVAAEWGGKHW
                     TVRMADGTSITARVLVWGTGALHEPSIPDIAGLDEFAGTVFHSARWNHDHDLRGKRVA
                     VLGTGASAIQFVPAIQPGVARLTVFQRTAPWVLPKPDRAVSPLWRRIYRAAPGVQKLQ
                     RALIYARNESLLTGFRKPRRMAVVEKFARGYLDRKFADRPDLKAKLTPDFTIGCKRIL
                     LSSDYYPALKQPNVEVVTEKVVRVTPTAVVTADGVEHEVDTIILGTGFKVADGLGRSV
                     RITGRDGVDLADAWRSGMQGFLGTTIAGFPNLVTLTGPNTGLGHSSMIYMIESQTNYL
                     LDALRLIDENGAVAIDTRRDRQDAFNATLQGEFAGSVWTQGGCASWYLDEHGRNRTLW
                     PSHTFTFRRRTRKVRPADHELLA"
     gene            1283..2230
                     /locus_tag="VFE39_04775"
     CDS             1283..2230
                     /locus_tag="VFE39_04775"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HET6257947.1"
                     /translation="MPDPAVYSIAMTVRFRGITTREGMLLQGPAGWGEFCPFTEYDDL
                     ESAPWLAAAVEAAEEGWPEPVRDRVPVNCIVPAVGPERAQEIVRASGCSTAKVKVADA
                     PDSLPADLERVAAVRDALGPGGSVRVDANARWEIEEAVTAIRALDRAAGGLQYVEQPC
                     RTIEELAAVRRRVDVPIAADESIRRAEDPLRVAVAGAADIAVLKCAPLGGVRRALAVA
                     EACGLPCVVSSALQTSVGLAGELALAGALPDLDLACGFGTGALLAADVVAEPLLPHDG
                     WLPVPRRAPEPVLRDAVAAGPERTRWWTDRLARVLAGVG"
     gene            complement(2247..2699)
                     /locus_tag="VFE39_04780"
     CDS             complement(2247..2699)
                     /locus_tag="VFE39_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6257948.1"
                     /translation="MTMAPDIIAPSTLPAGRLKSDINVKVKEIAETCMRIGALASRTG
                     VSQRLLRYYEEQGMLEPARLPSGYREYSEADVGTVRRIRSLLAAGLTTSVIAQILPCA
                     REETDRLVPVCPELAARLNGERARLTSTITQLQNSRRILDGVLAESES"
     gene            2692..3330
                     /locus_tag="VFE39_04785"
     CDS             2692..3330
                     /locus_tag="VFE39_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6257949.1"
                     /translation="MVIGAGPGIGRAVALRFGREGLPVSLVARTAATLSETAAVLAAQ
                     DRPVVTLTADVTDEGALRAAIDVAVQELGVPDVVVYNAAIIQSDAPGQLSARGQLDAW
                     AVNVVGALTAAAHVLPGMSARGGGSFIITGGMPEPKPEYVSLSLGKAGVRTLVELLDL
                     EYGKAAIHVATVTVAGAVAPGTAFDPDDIAEHYWTLHSQPRHEWMRELVHDG"
     gene            complement(3438..3812)
                     /locus_tag="VFE39_04790"
     CDS             complement(3438..3812)
                     /locus_tag="VFE39_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6257950.1"
                     /translation="MTVDHLVGLGHQRIAHIDGSRAPGATERRRGYCNAMRAHGITEH
                     EQVVRGGLTENDGATAAQALLNGRHPTAITVFNDRCATGVLDTLRNAGLAVPSDISIV
                     GNDNSRLGLVRRSTTAKGRPRP"
     gene            4032..4985
                     /locus_tag="VFE39_04795"
     CDS             4032..4985
                     /locus_tag="VFE39_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6257951.1"
                     /translation="MSVVVVGAGLAGVACAVELAAAGVPVRVLERAGSVGGRMASSTI
                     EGRPVDIGAAYFTVRDAEFAEVVARWRTAGLARPWTSELAVLGDGSRGRSPGPVRWAA
                     PHGLRSLVAELAAGLSLELQHEVRHVGPGPVVDGTAADVVVLAMPDPQALRLVEPASP
                     AVAELTGREWRPVVTVVAGWPSREWPEMNAAFVNDHPVLSLIADDGDRRGDGAPVLVA
                     HTTADVARAHDAEPRGAVEPVLGAIRELLDLHTQPAWTHAHRWRYAAPTADRDHPFHL
                     GDDGIALAGDGWGSARVETAWRSGTLLGRAVAARLGAGRSH"
     gene            5040..6470
                     /locus_tag="VFE39_04800"
     CDS             5040..6470
                     /locus_tag="VFE39_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6257952.1"
                     /translation="MAVPADVSDPAVPDRAVTDPVIADLLAKLPADRMLLDPDVVATF
                     LHDEAEWAEFGSASAVVRPRNTAEVAVVVAACARHGRPVVTRGAGTGLSGGANAVDGC
                     VVLSTERMREIIEINAAERLAVVQPGVVNDDLRAAVAEHALWYPPDPASAPWSTIGGN
                     VATNAGGLCCVKYGVTRDYVLALEVVTAAGEVVRVGRRTAKGVAGYDLVGLMVGSEGT
                     LGVITEITVRLRPLRTSPQRTVVGFFDTLAAAGDAVSRVTARGLQPAAFELIDRFCLR
                     AVNQWKNAGLPEEAAALLLAQTDLPEPAGLQEAEAIHAEFVAAGASDAMLSTDPVEAE
                     ALFAARRLAYPALEQLGQAMLTEDVCLPRGRLAEMLARIEEIATRHDTLIATVAHAGD
                     GNLHPLMLTPHGDEAAKLRAQAAFEEILDGALELGGTVTGEHGVGLLKLPGMRRELGP
                     QVVAMQRAIKAALDPQGILNPGKVVG"
     gene            6467..>6826
                     /locus_tag="VFE39_04805"
     CDS             6467..>6826
                     /locus_tag="VFE39_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6257953.1"
                     /translation="MNRLLPALLDPQDRIALRFGADHSTRTELSYAQLAGAAGALAAA
                     LAGRQRVAVHATPTLHTAVGITAALLAGVTAVPLNPRLGERELGHVLADSAPELVLAA
                     PGVVLPGLLAALPRLDID"
BASE COUNT          873 a         2409 c         2568 g          976 t
ORIGIN      
        1 ctggacccgc ctgttcgcgc aacagcccga gatccgcgcc tatctcgagg acatcgccga
       61 ccgctacggc ctgcgcgccc gcatccgctt ccgctccacg gtggtcgccg cggagtgggg
      121 tggcaagcac tggaccgtgc ggatggcgga cggcacgagc atcaccgccc gtgtactggt
      181 ctggggcacg ggagcgctgc acgagccgtc gatccccgac atcgccgggc tcgacgagtt
      241 cgccggcacg gtgttccatt cggctcgctg gaaccacgac cacgacctgc ggggcaagcg
      301 cgtggccgtg ctcggcacgg gcgcgagcgc catccagttc gtgccggcga tccaaccggg
      361 cgtcgcccgg ctcaccgtct tccagcgcac cgcgccgtgg gtgctgccca agccggaccg
      421 cgcggtgtcg ccgctgtggc ggcggatcta ccgggccgcg ccgggcgtcc agaagctgca
      481 gcgggccctg atctacgcgc gcaacgagag cctcctgacc gggttccgca agccgcgccg
      541 gatggcggtc gtcgagaagt tcgcgcgcgg ctacctcgac cgcaagttcg ccgaccgccc
      601 ggacctcaag gccaagctca ccccggactt caccatcggg tgcaagcgca tcctgctgtc
      661 cagtgactac tacccggcgc tcaagcagcc caacgtagag gtcgtcacgg agaaggtcgt
      721 gcgcgtcacg cccaccgccg tggtcaccgc cgacggcgtc gagcacgagg tggacacgat
      781 catcctcggc accggcttca aggtggccga cggcctcggg cgctcggtcc gcatcacggg
      841 ccgggatggg gtggaccttg ccgacgcctg gcggagcggc atgcaggggt tcctgggcac
      901 cacgatcgcg gggttcccca acctcgtcac gctgaccggt cccaacaccg gcctgggtca
      961 cagctcgatg atctacatga tcgagtcgca gacgaactac ctgctcgacg cattgcggct
     1021 gatcgacgag aacggtgcgg tcgccatcga cacgcgccgc gaccgccagg acgccttcaa
     1081 cgccaccctc cagggcgagt tcgccggctc ggtgtggacg cagggcggct gcgcgagctg
     1141 gtatctcgac gagcacggca ggaaccgcac tctctggccg agccacacct tcacgttccg
     1201 ccgccgcacc cggaaggtcc gaccggccga ccacgagctg ctggcctgac tccaggtccg
     1261 ggcgttcgcc tagggtcgga ttgtgcccga cccagccgtc tactccatcg ccatgaccgt
     1321 ccggttccgc gggatcacca cgcgggaggg gatgctgctg caggggccgg cagggtgggg
     1381 cgagttctgc cccttcaccg agtacgacga cctcgagtcg gcgccgtggc tggcagcggc
     1441 tgtcgaggcg gccgaggagg gctggccgga gcccgttcgc gaccgggttc cggtgaactg
     1501 catcgtcccg gcggtcgggc cggagcgggc ccaggagatc gtccgggcgt ccggctgctc
     1561 cacggcgaag gtcaaggtcg ccgacgcgcc cgactcgctt ccggccgacc tggaacgcgt
     1621 ggcggcggtc cgcgacgcac tcggcccggg cggctctgta cgggtggacg ccaacgcccg
     1681 ctgggagatc gaggaggctg tcaccgcgat ccgggcgctg gaccgtgcgg cgggcggcct
     1741 gcagtacgtc gagcagccgt gccgcacgat cgaggagctc gccgcggtgc gccgccgggt
     1801 cgacgtaccg atcgcggccg acgagtcgat ccggcgtgcc gaggacccgc tccgggtggc
     1861 cgttgccggt gcggccgaca tcgccgtgct caagtgcgcc cccctgggtg gggtgcggcg
     1921 cgcactcgcc gtcgccgagg catgtggctt gccgtgcgtg gtgtcgtctg cgctgcagac
     1981 cagcgtcggg ctggcgggag agctcgcgct ggccggcgcg ctgcccgacc tggacctggc
     2041 gtgcgggttc ggcaccggtg cgctgctcgc cgccgacgtc gtcgccgaac cgctcctgcc
     2101 tcacgacgga tggctccccg tgccgcgccg cgcgcccgaa cccgtgctcc gcgacgccgt
     2161 ggccgccggg cccgagcgga cccggtggtg gacggatcgg ttggcacggg tgctggcggg
     2221 agtggggtga ccgctgtccc gcggcctcag ctctcgctct cggccagcac gccgtcgagg
     2281 atcctgcggg agttctgcag ctgggtgatc gtgctggtga gccgggcccg ctccccattg
     2341 agccgcgctg ccagctcggg gcataccggc accagccggt cggtctcctc gcgagcgcac
     2401 ggcaggatct gggcgatcac ggacgtcgtg aggccggcag cgagcaggct gcggatgcgc
     2461 cggaccgtgc cgacatccgc ctcggagtac tcccggtagc cgctgggcag ccgcgccggc
     2521 tcgagcatgc cctgctcctc gtagtagcgc agcaagcgct ggctgacccc ggtcctgctt
     2581 gccagcgcac cgatccgcat gcaagtctcc gcgatctcct tgaccttcac attgatgtca
     2641 gacttcagcc tcccggcagg caacgtcgaa ggagcaatga tgtcgggagc catggtcatc
     2701 ggagcaggtc cgggtatcgg ccgagcggtc gccctccggt tcggacggga gggcctgccg
     2761 gtctcgctcg tggcccgaac cgcggcgacg ctctccgaga cggctgcggt tctcgccgcg
     2821 caggacaggc cagtcgtgac gctgaccgcc gacgtcaccg acgagggggc gttgcgtgcg
     2881 gcgatcgacg tcgcggtcca ggagctcggc gtccccgacg tcgtcgtcta caacgcggcg
     2941 atcatccaat cggacgcgcc gggccagctg tcggcgcgcg gtcagctcga cgcctgggct
     3001 gtcaacgtgg tcggcgccct caccgcggcg gcgcacgtcc tgcccggaat gtccgcgcga
     3061 ggcggtggtt ccttcatcat caccggagga atgccggaac cgaagccgga gtacgtcagc
     3121 ctgtcgctcg gcaaggcggg cgttcggacc ctcgtggagc tgctggacct ggagtatggg
     3181 aaggccgcca tccacgtcgc caccgtgacg gtggccggcg ccgtcgcgcc gggcaccgct
     3241 ttcgacccgg acgacatcgc cgagcactac tggacactgc actcgcagcc gcggcacgaa
     3301 tggatgcgag agctcgtgca cgacggttga tcaccgggcc gtcttgacgc gtgggcctgt
     3361 cagcgtcgca tcggccgtga gggcgaggtc agctgacccg ggtgccccct cgccgagtcc
     3421 gcggtgcatg tgcgtcgtca ggggcggggg cggcccttcg ccgtggtgct gcggcggacg
     3481 aggcccaggc ggctgttgtc attgccgacg atgctgatgt cggatgggac ggcgagcccg
     3541 gcgttgcgga gggtgtcgag gacgccggtg gcgcagcggt cgttgaacac ggtgatcgcg
     3601 gtcgggtgcc tgccgttgag gagtgcttgt gcggcggtgg cgccgtcgtt ctcggtgagg
     3661 ccgccccgca cgacctgctc gtgctcggtg atgccatggg cgcgcatggc gttgcagtag
     3721 ccgcggcggc gttcggtcgc gccaggggct cggctgccgt cgatgtgggc gatgcgctgg
     3781 tggccgagac cgacgaggtg atcgacggtc atctgaaggc cggcgtcgtc cgtgcgactc
     3841 gccggcggga atggacgact cgtggggtgg aaacgggcga ctcgcggggt ggggtcgggc
     3901 gactcgccgg tgggggtcgg gactcgcggg gccgagatcg ggcgactcgt gggcggggat
     3961 cgggcgactc gtgggcggga atgggcgact cgcgggggcg gggtgggggc gggggcaata
     4021 ccgtcatcgg cgtgtcggtg gtggtggtcg gagccgggct ggcgggtgtt gcgtgcgccg
     4081 tggagctggc tgctgccggg gtgccggtcc gggtgttgga gcgcgcaggg tccgtcgggg
     4141 gtcggatggc cagcagcacg atcgagggtc ggcccgtcga catcggggcg gcctacttca
     4201 cggtgcggga tgcggagttc gccgaggtcg tggcccgctg gcggaccgcc ggcctcgcgc
     4261 ggccgtggac gtccgagctc gccgtcctcg gcgacggcag ccggggccgg tcgccgggac
     4321 ccgtgcgctg ggcggcgccc cacggactgc gcagcctcgt cgcggaactg gccgccgggc
     4381 tgagcctcga gctgcaacac gaggtacggc acgtggggcc ggggccggtg gtggacggca
     4441 cggccgcgga tgtcgtcgtg ctcgcgatgc ccgacccgca ggcgctgcga ctggtcgagc
     4501 cggcctcgcc cgcagtggcc gagctgaccg gccgcgagtg gcggccggtc gtcaccgttg
     4561 tcgccggctg gccgtcgcgg gagtggcccg agatgaacgc cgcgttcgtc aacgaccacc
     4621 cggtgctgtc cctgatcgcc gacgacggcg accgtcgcgg cgacggcgct ccggtcctgg
     4681 tcgcccacac caccgccgac gtcgcccgcg cgcacgacgc cgagccgcgc ggtgccgtgg
     4741 agccggtcct cggtgcgatc cgggagctcc tcgacctgca cacccagccc gcctggaccc
     4801 acgcccaccg ctggcgctac gccgccccca ccgccgaccg ggaccatccg ttccacctgg
     4861 gcgacgacgg catcgcgctc gccggcgacg gatggggcag cgcccgggtc gagacggcgt
     4921 ggcggtccgg gacgctgctg ggccgggcgg tagctgcgcg cctcggcgcc ggccggtcgc
     4981 actgagtgat tcgcctcccg tcaggcgcag attcgccggg ggatgactag cgtgctgcca
     5041 tggccgtacc cgccgatgtg tccgaccccg cggtgcccga ccgcgcagta accgaccccg
     5101 tgatcgccga cctgcttgcg aaactccccg ccgaccggat gctcctcgat cccgacgtcg
     5161 tggccacgtt cctgcatgac gaggccgagt gggccgagtt cgggagtgcc tccgccgtgg
     5221 tccggccgcg aaacacggcc gaggtcgccg tggtggtcgc tgcgtgcgcc cgacatggcc
     5281 gcccggtcgt gacgcgcggc gccggcacgg gcctgtccgg tggcgcgaac gccgtcgacg
     5341 gatgcgtggt gctgtccacc gagcggatgc gggagatcat cgagatcaac gcggccgagc
     5401 ggctcgccgt ggtgcagccc ggcgtcgtga acgacgacct gcgcgccgcc gtcgccgagc
     5461 acgccctctg gtacccaccc gacccggcga gcgccccctg gtcgacgatc ggggggaacg
     5521 tcgccaccaa cgcgggcggc ctgtgctgcg tgaagtacgg ggtcacgcgc gactacgtgc
     5581 tcgcgctgga ggtcgtcacg gcggcgggcg aggtggtgcg cgtgggcagg cgcaccgcca
     5641 agggcgtggc cgggtacgac ctggtcggcc tgatggtcgg gtcggagggc accctcgggg
     5701 tgatcaccga gatcacggtg cggctgcgcc cgctgcggac gagcccgcaa cgcacggtcg
     5761 tcgggttctt cgacaccctc gctgccgcgg gcgacgccgt atcccgtgtc acggcacggg
     5821 ggctgcagcc cgcggcgttc gagctgatcg accggttctg cctgcgcgcg gtcaaccagt
     5881 ggaagaacgc cgggctcccg gaggaggccg cagcgctgct gctcgcgcag accgacctgc
     5941 ccgagcccgc ggggctgcag gaggccgagg cgatccacgc cgagttcgtg gcggccgggg
     6001 cgagcgacgc gatgctgtcg accgacccgg tcgaggccga agcgctgttc gccgcgcgcc
     6061 ggctcgccta cccggcgctc gagcagctcg gccaggcgat gctcaccgag gacgtctgcc
     6121 tgccgcgggg gaggctcgcc gagatgctcg cccgcatcga ggagatcgcg acgcgccacg
     6181 acaccctgat cgccaccgtc gcgcacgcgg gcgacggcaa cctgcacccg ctgatgctca
     6241 ccccgcacgg cgacgaggcc gcgaagctgc gggcccaggc ggcgttcgag gagattctgg
     6301 acggtgcgct cgagctcggt ggcaccgtca ccggtgagca cggtgtcggg ctgctgaagc
     6361 tgccggggat gcgtcgcgag ctcggcccgc aggtcgtcgc gatgcagcgg gcgatcaaag
     6421 cggccctgga tccgcagggg atcctcaacc cgggcaaggt ggtcgggtga accgtctcct
     6481 ccccgccctc ctcgatccgc aggaccgcat cgcgctgcgc ttcggcgccg atcactcgac
     6541 ccgcaccgag ctgagctacg cgcagctggc cggcgccgcg ggcgcactgg ccgccgcgct
     6601 ggccggccgg cagcgggtgg ccgtgcacgc caccccgacc ctgcacaccg cggtcggcat
     6661 caccgcggcg ttgttggccg gcgtcacggc ggtgccactg aacccgaggc tgggggaacg
     6721 ggagctcggg catgtcctgg ctgactcggc gcccgagctc gtcctcgctg cacccggcgt
     6781 cgtgctcccc gggctgctcg cggcccttcc ccgtctcgac atcgat
//
LOCUS       DASRSC010000099         5210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_31034,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000099 DASRSC010000000
VERSION     DASRSC010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31034"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            514..2115
                     /locus_tag="VFE39_04810"
     CDS             514..2115
                     /locus_tag="VFE39_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6257954.1"
                     /translation="MDSPTHRPEEWAQAHVADLVTQAAARVPHNPAIVDVTSGITLTW
                     RELDGAVSAEAARLRDVGVAAGDRVLVRLGNGAEFCVAVLGALRADAVTVPFGSIGVA
                     RELDIVLSDCRPAVVVAAEGDDTARASAAALNSAVIVPPDVRARAEGPSQGDARRGGE
                     EIALLVYTSGTTGSPRGVRLSHRALLANRAQTAALRPAPVIPVDRVLLSLPMFHIFGL
                     AAGLLQACWAGATVVLVERFEPAHIAEVLVEQRVSGLAGVPSMFRSLLDLPAGELRAA
                     TAGVRLCTSGGAPLPAEWLADFRAATGLTIVEGYGLTEAGPVLTTTPTAGVPKPGSVG
                     RPLPGVELRLVDREGTPLDSGSEPDPEDLDDLDDLDDVDGLDSDVFDAQDTGLVAVRG
                     PNLFSGYWPDGAGGPDDEGWFRTADVGFLDADGDLHLVDRSSDVVIVNGFNVYPREVE
                     QVLVELPGVAEGAVVGVPDDRTGEAVKAVLVRVPDAELTEEDVRAHCVERLARFKVPT
                     VIEFADALPRTPTGKVARRQLAGQS"
     gene            2112..2384
                     /locus_tag="VFE39_04815"
     CDS             2112..2384
                     /locus_tag="VFE39_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HET6257955.1"
                     /translation="MRVPHEVAPQQAGPTRVTVYTRAGCTACATAEAEVARICGELGH
                     GWVAVDVDSDVDLQAEYGDRVPVIMIDGREHGFWKVEEARFRAALA"
     gene            2458..4047
                     /locus_tag="VFE39_04820"
     CDS             2458..4047
                     /locus_tag="VFE39_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HET6257956.1"
                     /translation="MSTTDAPPHPARPTRHGIPRGFAALIGVLAVGAAVGVGHLVAGI
                     VSPASSPYLAVGDTVIRFSPQSLTEFAKTTFGTSDKPVLLVGVGVVLLILAVVAGITS
                     RYSRTPGVVVIGALGVLGLLAVVSAPVFAPLDVLAPLASLVAGLTVFRRLHALALLLQ
                     DAQDAPVHRTGVSRRTVLIGSSAVVGVMSLAAAVGGQLLGRGIADSRRGVTARLAGAR
                     LAERASAIPATAAFPELGTPTFLTANPDFYRIDVALRIPERAAEDWSMRIHGMVDKEI
                     MLRFDDLLARPLVERTITMTCVSNPVGGNLISTANFIGVDLREILLEAGVRPGADQLF
                     TTSIDGFTAGTPTSVVMEEGRGALLAIGMNGEALPPEHGFPVRMVVPGLYGYVSATKW
                     ITDLAVTTFGAEQSYWLERGWAAQGPIKTESRIDTPRGFASVQAGRVTVAGIAWSQPA
                     GIGKVEVRMDGGAWREAELATEVSGDTWRMWRADFDLGPGSHTVQTRATDSAGRTQTE
                     VQADPVPDGASGWPATIFTAT"
     gene            4269..4946
                     /locus_tag="VFE39_04825"
     CDS             4269..4946
                     /locus_tag="VFE39_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HET6257957.1"
                     /translation="MRKIPRLATVGIMAALTVALGACSGPETPSAGAPASAPAAAPMS
                     APASADDGVTTANDVFGPACNMLPRGDAPGSLNKMGPQPVANAASANPLLTTLVSAVR
                     AVPGLADTLNAQQGVTVYAPSNAAFDELKAAIGDQAFTALLNNPQRLGALLSYHVVPK
                     RYDAAGLVEAGRTTELAGGDVTIGGTAEAPTLTSGNGTQARVLCGNVPTSNATVFVID
                     KVLMPAS"
BASE COUNT          689 a         1791 c         1932 g          798 t
ORIGIN      
        1 cgggggagcg gtgggccgtc ggcgtggggt ggccgaggtg gcccgggtgg tgcgccgacg
       61 cggggagcgg tggtcggggg catctctacc tctccagccg ccggggaacg ggcggtcttg
      121 gtggggagca gccggggctg acgcgaggat cgagtcactc ccggctggtc ctgtagccgt
      181 gacggacagg acgccgcggg acgacccgcg gtgtgctggc ggcaacaggt tgcatgccac
      241 tcccttgggt gagtctcggg ggcgatcacg agtgtcgagg cgtcgagctg cgtgtagcgg
      301 cggagcatac gaccgcagca ttgtgacgaa ggaggcagca ccgctcgccg tcggcatgtt
      361 ccggtgagcg gccaactaca cgcggtcaac gtcatgacag ggtgcgtgtc gtccggcgac
      421 gggctgcacg agttgcgatg agcgtcgtcg cggccgcgct gcacggttcg tgccagactc
      481 gccccgacgc ttcgcgccaa cccctggaga actgtggaca gcccgaccca ccgccccgag
      541 gagtgggcgc aggcacacgt ggcggatctg gtgacccagg ctgccgcccg ggtgccgcac
      601 aaccccgcga tagtggacgt gaccagcgga atcacactca cctggcgaga gctggacggt
      661 gccgtctccg ccgaggccgc ccgcttgcgc gacgtcggag tggctgccgg cgaccgcgtg
      721 ctggtgcggc tcggcaacgg ggcggagttc tgtgtggcgg tgctgggcgc gttgcgggcc
      781 gacgccgtga ccgtgccgtt cggctcgatc ggcgtggcca gggagctgga tatcgtgctc
      841 tccgactgcc ggcctgccgt cgtcgtggct gcggagggtg acgacaccgc tcgggcgagt
      901 gcggccgcgc tcaactcggc agtgatcgtt ccgcccgacg tgagggcgcg cgccgagggg
      961 ccctcgcagg gggacgcccg acggggtggg gaggagatcg cgcttctcgt ctacacctcg
     1021 ggcaccaccg gcagtccccg cggtgtccgg ctctcgcatc gggcgctgct cgccaaccgt
     1081 gcgcagaccg cggcgctgcg tcccgcgccg gtgatccccg tggaccgggt gctgctgtcg
     1141 ttgccgatgt tccacatctt cggcctggct gccgggctcc tgcaggcgtg ctgggcgggg
     1201 gccaccgtcg tgctcgtcga gcggttcgag cccgcccaca tcgccgaggt gctcgtcgag
     1261 cagcgggtga gcgggctggc gggagttccg tcgatgttcc gctcgctgct cgacctcccg
     1321 gcaggcgagc tgcgcgccgc caccgcgggc gtccggctgt gcacgtcggg cggcgccccg
     1381 ctgccggcgg agtggctcgc ggacttccgc gcggccaccg gcctgacgat cgtcgagggg
     1441 tacggcctca ccgaggcggg gccggtgctg accaccaccc cgacggccgg cgtccccaag
     1501 cccggatcgg tgggccgccc gctgcccggt gtcgagctgc ggctcgtgga cagggagggc
     1561 acgccgctcg acagcggctc cgaacctgac cccgaggacc tcgacgatct tgatgatctc
     1621 gacgatgtgg acggcctcga cagcgacgtc ttcgacgcgc aggacaccgg gctggttgcc
     1681 gtgcgggggc cgaacctgtt ctccgggtac tggccggacg gggccggtgg gccggacgac
     1741 gagggctggt tccgtaccgc cgacgtcggc ttcctggatg ccgacggcga cctgcacctc
     1801 gtcgaccgct cgtccgacgt cgtgatcgtc aacggcttca acgtctaccc gcgcgaggtg
     1861 gagcaggtgc tggtggagct gcccggggtg gcggagggcg cggtcgtggg ggtgccggac
     1921 gaccgtaccg gcgaggctgt caaggcggtt ctggtgagag tcccggacgc cgagctcacc
     1981 gaggaggacg tgcgggcgca ctgtgtcgag cggctggccc ggttcaaggt tcccacggtc
     2041 atcgagttcg ccgacgcgct gccgcggacg cccacgggca aggtcgcgcg gcggcagctg
     2101 gcaggacagt cgtgagggtc ccgcacgagg tcgccccgca gcaggccggg ccgacgaggg
     2161 tcaccgtgta cacgcgggcg gggtgcacgg cgtgcgccac tgccgaggcc gaggtggcgc
     2221 ggatctgcgg cgagctcggc catggctggg tggccgtcga cgtcgactcc gacgtcgacc
     2281 tgcaggccga gtacggcgac cgggtaccgg tgatcatgat cgacggccgc gaacacggct
     2341 tctggaaggt cgaggaagca cgttttcggg ctgcgctggc ctgatcctgc cggcccgtca
     2401 gcggatcgtc acacttccgg ggtgaaccgg acgggccggg cgggcgaaac ttcctgcgtg
     2461 agcacgaccg acgcaccacc tcatcctgcg cgccccacgc gacacggcat cccacgcggg
     2521 ttcgccgcgc tgatcggggt gttggccgtc ggtgcggcgg tcggcgtcgg gcacctggtg
     2581 gccggcatcg tctcgcccgc gtcctcgccg tacctggccg tcggcgacac ggtgatccgc
     2641 ttctcgccgc agtccctcac cgagttcgcc aagaccacct tcgggacgtc cgacaagccc
     2701 gtcctgctgg tcggtgtggg tgtggtcctc ctcatactgg ccgttgtcgc gggcatcacc
     2761 tcgcgctaca gccggacccc cggtgtggtc gtgatcggcg cgctgggtgt gctcggcctc
     2821 ctcgcggtgg tcagcgcacc ggtcttcgcc ccgctcgacg tcctcgctcc gctcgcgtcg
     2881 ctggtcgcag gcctcactgt gttccgcagg ctgcacgcgc tcgccctact gctccaggac
     2941 gcgcaggatg cgccggttca ccgaaccggg gtgtcccggc gcacggtgct catcggctcg
     3001 tcggcggttg tgggcgtgat gtcgctcgca gcggccgtcg gtggccagtt gctcggccgc
     3061 ggcatcgccg actcccggcg gggggtgacg gcgcggctcg ccggggcccg gctcgccgaa
     3121 cgtgcctccg cgatcccggc gactgcggcc ttccccgagc tgggcacccc gacgttcctc
     3181 accgcgaacc ccgacttcta ccggatcgat gtggccctgc ggatccccga gcgggcggcc
     3241 gaggactggt cgatgcggat ccacgggatg gtcgacaagg agatcatgct gcggttcgac
     3301 gacctgctcg cccgcccgct cgtcgagcgc acgatcacga tgacgtgtgt ctccaacccg
     3361 gtcggtggaa acctgatctc gacggcgaac ttcatcggcg tggacctgcg cgagatcctg
     3421 ctcgaggcgg gcgtccggcc cggtgccgac cagctgttca ccaccagcat cgacggcttc
     3481 accgccggca cgcccacgtc cgtggtgatg gaggaaggcc gcggcgcgct gctcgcgatc
     3541 gggatgaacg gtgaggcgct gccgccggag cacggctttc cggtgcgcat ggtcgttccc
     3601 ggcctctacg gctacgtctc ggccaccaag tggatcaccg acctggcggt cacgacgttc
     3661 ggcgccgagc agagctattg gctcgagcgc gggtgggccg cgcagggccc gataaagacg
     3721 gagtcgcgca tcgacacccc gcgcgggttc gcctccgtac aggccgggcg ggtcacggtc
     3781 gccggcatcg cctggtcgca gccggccggg atcggcaagg tcgaggtgcg gatggacggt
     3841 ggtgcgtggc gggaggccga gctggccacc gaggtgagcg gggacacctg gcggatgtgg
     3901 cgggctgact tcgacctggg gccggggagc cacaccgtcc agaccagggc caccgactcg
     3961 gccggccgca cccagacgga ggtgcaggcc gacccggtcc cggatggcgc gtccggatgg
     4021 ccggcgacga tcttcaccgc cacctgaccg cttcacagcc cgagcccggc cgcgggctga
     4081 accgccggtc gctcggcgtc cgccgttcga cggatccggc cgctcctcgg ccgcgccgcc
     4141 ggacgaccgt ggaggggtgc tcgcgtcgtt atgtaagcgt tatctgtggg atgtgaacca
     4201 ccttcggcgc gaaaccgaag tgtggtacca ccccggacca gccgggcgtg cccgagggag
     4261 gaacctccgt gcgaaagatt ccgagactgg ccacggtcgg catcatggcg gccctgaccg
     4321 tggcgttggg ggcctgtagc ggccccgaga cgccgtctgc gggcgcaccg gccagcgccc
     4381 ccgccgctgc gccgatgtct gccccggcgt ccgcggacga cggcgtcacc accgcgaacg
     4441 atgtgttcgg tccggcctgc aacatgctgc cgcgaggcga cgcgcccggt tccctgaaca
     4501 agatggggcc gcagcctgtg gcgaacgccg ccagcgccaa tccgctgctg accacgttgg
     4561 tgagtgcggt ccgggctgtt cccggcctgg ccgacaccct caacgcgcag cagggggtta
     4621 cggtctacgc cccctccaac gctgcgttcg acgagttgaa ggccgcgatt ggcgaccagg
     4681 ctttcaccgc gctgctgaac aacccgcagc ggctcggcgc cctgttgtcc taccacgtgg
     4741 tcccgaagcg gtacgacgct gccggcctgg tggaggcggg ccggaccacc gagctcgcgg
     4801 ggggcgacgt caccatcggt ggcaccgctg aggcgcccac gctgaccagc ggaaacggca
     4861 cgcaggccag ggttctctgt ggcaacgtcc cgacctcgaa cgccaccgtg ttcgtgatcg
     4921 acaaggtgct catgccggca agctgatctc gctctgcacg acaccgcagg ggcggtcgcg
     4981 gtcgtcgggg ccgccctccg gcaactgtgc tgcacgacga gcgggcgtcg tggggcgatc
     5041 accggtagta atgtgccgag cacccacgcg agcacgactg catccaagat cgcaactttg
     5101 tgcgtgcctt cacaagcgac taccgtgtaa tccagtcgac gtcaacgccc aatcctgggg
     5161 tactcggccg ggtgtatacc ggttggtggg gtctgtggtg cggtcgcgcg
//
LOCUS       DASRSC010000100         8030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3633722,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000100 DASRSC010000000
VERSION     DASRSC010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3633722"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VFE39_04830"
     CDS             complement(<1..246)
                     /locus_tag="VFE39_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257958.1"
                     /translation="MQVVVAQQADLGQVVDQLAVDVQHQIGHGRVGEGLLRADRDPEL
                     GLAEAAHPASQTSSWASSWSSAVAALPAYSQVSYDAAG"
     gene            365..826
                     /locus_tag="VFE39_04835"
     CDS             365..826
                     /locus_tag="VFE39_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014174167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6257959.1"
                     /translation="MSRHIQVTFDAHDPRALSSFWRDALGYVHPGPPGVDLPEDADPL
                     AAWDDFLARVGVPEEQRNTRSAIQDPDGHGPRLFFQQVPEEKVAKNRIHLDVRAAPGL
                     QGEVRMAALEVECDRLVALGATRVRRYEPAPPMSAGFIVMTDPEGNEFCLD"
     gene            complement(938..1351)
                     /locus_tag="VFE39_04840"
     CDS             complement(938..1351)
                     /locus_tag="VFE39_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6223 family protein"
                     /protein_id="HET6257960.1"
                     /translation="MSVRHLIAAADAALLGGFGLAAPAAAHVSVAAGVIGTGSGRLGA
                     IVAAVVGLIGVVIGGLALARSAGRIGTGSGRSGAIVALVVGLIGMVLAGLHLATSTGG
                     IGTGNGRAGAIVALVVGLLGVVIGGLVLARSRRTA"
     gene            1660..2877
                     /locus_tag="VFE39_04845"
     CDS             1660..2877
                     /locus_tag="VFE39_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6257961.1"
                     /translation="MVSTMREAVRRGELSPVVADASLGVGVALGLLAAAFAGVGPAAA
                     ASPRPLDAGAVALAATVAVAVAVRRRYPIAALVVLNTVPLVWFLGAYPGRLITLAPLI
                     GCYTLAAYRGWRWGLAGAVPTALIQIIAIRVVLGDTETAGVVPEVALLVATAGSAGAA
                     VGYNRAVLAATRAQFARETQSREERTRRIAVEERLRIARELHDVFGHTMATISVQAGV
                     GLHVIEQRPGQAREALAAIKKICDDGLTDVKTILGILRADTSTEEEPEQPRAPRGGLD
                     RLTELLDTAEAAGLRVELNVDGDPRPLPAPVDLAAYRIIQEALTNVLRHAEARTVRLA
                     LKHEPSRLLIRIRDDGPTTSADTHHTAAGHYRHGIDGMRERARALSGQLTATPHPEGG
                     FEVCAELPVPHPR"
     gene            2960..3640
                     /locus_tag="VFE39_04850"
     CDS             2960..3640
                     /locus_tag="VFE39_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6257962.1"
                     /translation="MTEPIRVLIADDEALVRGGFRVLIDNEPGMIVVGEAGDGATAVD
                     LARHTRPDVVLMDIRMPGVDGLRATHQIAADPDLADVHVLILTTFDHDEYVIEALGAG
                     AAGFLIKNTEPRQLLHGIRVVTGGEALLSPGLTRRLIAQLANRTTSPRLNTDALAQLT
                     TREKQIVGLVAHGLSNTDIATALTISHATAKTHVSRAMAKLGARDRAQLVVLAYQHRL
                     VEPPAPTP"
     gene            complement(3664..4008)
                     /locus_tag="VFE39_04855"
     CDS             complement(3664..4008)
                     /locus_tag="VFE39_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257963.1"
                     /translation="MDLRRLLPALALGALLAGCGGGGVSAGPLAPHALDGPDYSGYLA
                     AVEGIPFLRGPHAAATAACERKARDMTREEAIDLVARAMAGVGFGRPEAQRTIDAVDE
                     FNVCPSSTTWES"
     gene            complement(4218..5393)
                     /locus_tag="VFE39_04860"
     CDS             complement(4218..5393)
                     /locus_tag="VFE39_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019811771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6257964.1"
                     /translation="MTTAVDWQARAHGLVEKLTEAGVLDDPAWRDAVESVPRHVFVPT
                     FYVQQPGGDWSATSAGSDGWLDAVYDDRPLVTALAENAAGGRVTMSSSSQPALMLRML
                     AALDVRLGHRVLEIGTGTGYNAGLLAHRLGDGQVFSVDIGAALVEAARERLAGLGYAP
                     TLAAVHGAFGLPDQAPFDRIIATCSVPVVPWAWAEQLRDGGLVLVDVKRSAHTGNLAL
                     LRRYPDRLEGRFLPKWATFMAIRDTDAAPPTLTGVTMQPEVGWRSSTQLDPQPWTAVV
                     PWFLASSRLPQRLVFGYRGRTETGSEWATFRGGDGSWCMVRMLPDERGRREVCEGGPI
                     GIWTQFERAHDQWDMLGRPGWDRLGLTVRQDGSHRVWLDEPDGACSWLVTGGRDAAR"
     gene            complement(5899..6642)
                     /locus_tag="VFE39_04865"
     CDS             complement(5899..6642)
                     /locus_tag="VFE39_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6257965.1"
                     /translation="MGCVDCRDAISARLDGEDLPGESEAIDAHVASCADCSRYADRAA
                     RITRLTRTRAAESGPDLVAVVLDAAPPTPRRRLHPGPVRLALGVVGLGQCALAINGIL
                     AVGAHHGVVELAGASAAHLSHESSAWNLALAIGFLWVATGTARPAGLVPLVGAFVGVL
                     TVLSVLDVLRGGVDPSRLWTHGLIVLGFVLLLLLQRITRHGGGGAIRSGPLDRQLAEH
                     RPPEGVLRSLHGGQDEENGGLQPTAHGAA"
     gene            6706..7287
                     /locus_tag="VFE39_04870"
     CDS             6706..7287
                     /locus_tag="VFE39_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6257966.1"
                     /translation="MVERPDDARITALALAAGRGDRTALAEFVRATQRDVHRFVTYLS
                     GPGDADDLAQETYLQALRSLPRFANRSTARIWLLSIARHTAADAVRIAVRRPRTSGVD
                     DWEALAAATGSRELVRSAGDEAVLLRSLVDRLDLDRRQAFVLTQLLDLSYADAAEICG
                     CPVGTIRSRVARAREDLVEAVADTRGSRRAGQG"
BASE COUNT         1271 a         3009 c         2690 g         1060 t
ORIGIN      
        1 gccggcggcg tcgtacgaga cctgggagta cgccggcagc gcggccaccg cgctcgacca
       61 gctcgaggcc cagctcgacg tctggctggc cgggtgcgcg gcctcggcga ggccgagctc
      121 cgggtcccgg tcggcgcgaa gaagcccttc cccgacgcgc ccatggccga tctggtgctg
      181 cacgtccacc gcgagctgat ccaccacctg tccgaggtct gcctgctgcg cgaccactac
      241 ctgcatacga aacccgctac gaacggagca acccgatgat cgagcctgcg agcgaatcat
      301 cagcacagcg cccgcgcgct gcgttgtttc acgatgggcc ggccgagcga agcgaggacg
      361 tacgatgagc cgccacatcc aggtcacctt cgacgcccac gacccgcggg cgctgtcgtc
      421 cttctggcgc gacgcactgg gctacgtcca ccccggcccg cccggagtcg acctgcccga
      481 ggacgccgac ccgctagcag cgtgggacga cttcctcgcg cgggtcggcg taccggagga
      541 gcagcgcaac acgagatcgg ccatccagga cccggacgga cacggcccac ggctgttctt
      601 ccagcaagtg ccggaggaga aggtcgccaa gaaccgcatc cacctcgacg tccgagcggc
      661 tcccggactg cagggcgagg tgcggatggc ggcgctggag gtcgagtgcg accggctcgt
      721 cgcgctggga gcgacgcggg tacgtcgcta cgagcccgct cccccgatga gcgccggctt
      781 catcgtgatg accgaccccg agggcaacga gttctgcctg gactgacaca gctcagctgt
      841 agtcacagaa gccgcggccg gtcttgcggc cgaggtagcc ggctgaagcc gtcggtgggg
      901 acgggcactg acccggcgcc atggtcgggt ttgccggtca ggcggtgcgg cgggagcggg
      961 ccagaaccag cccaccgatg accacgccga gcagccccac caccagggcc acgatggccc
     1021 cggctcgccc gttgccggtg ccgataccac cggtggaggt ggccagatgc agcccagcga
     1081 ggaccatgcc gatcagcccc accaccaggg ccacgatggc cccgcttcgc ccggagccgg
     1141 tgccgatacg accggcggag cgggccagag ccagcccacc gatgaccacg ccgatcagcc
     1201 ccaccaccgc ggccacgatg gccccgagcc gcccggagcc ggtgccgatg acaccggctg
     1261 cgacggagac gtgcgcggcc gccggtgcgg caagcccgaa cccgccgagc agggcggcat
     1321 cggcggcggc aatcaggtga cggacggaca ttgaggatcc cctcgcttga gtcgtggcgt
     1381 catcgggtag cggttggaac agttcgacga cgttgcctgc ggggtcttgc agcaggatct
     1441 gttgtcgatg gtggttgccg ggctggggcc tgcgcgtcgc gcgacggtgg tgtttcccgg
     1501 ctgatgccct cggcgtacgg tcagccgccg gtagtagacg cattcgaccg gtgtggtact
     1561 gccgagacac tgcgccaggt ggatgcacgc ccgcggtcgc acgcgcaaca tgggacaggg
     1621 cggcgtgctc tcggttgagc tcggatcgag ggcgggagaa tggtcagcac catgcgtgag
     1681 gcggtgcgcc gcggcgagct ctcgccggtt gtggccgacg cgagtctcgg cgtcggtgtg
     1741 gcgcttgggc tcctcgccgc cgctttcgcc ggggtcggac ccgcggcggc cgcgagccca
     1801 cgcccgctgg acgccggagc ggtcgcgctg gccgcgacgg tcgccgtcgc ggtcgcggtg
     1861 cgccgccgct acccgatcgc ggcgctggtc gtgctcaaca cggtccccct cgtctggttc
     1921 ctcggcgcct atcccgggcg gctgatcacg ctggccccgc tgatcggctg ttacacgctc
     1981 gcggcctacc ggggctggcg gtggggtctg gcaggcgccg tgcccaccgc gctgatccag
     2041 atcatcgcga tccgagtggt gctcggcgat acagagaccg ccggtgtggt ccccgaggtg
     2101 gcgctgttgg tggccaccgc gggcagcgcc ggcgcggccg tgggctacaa ccgggcggtg
     2161 ctcgcggcca cccgcgccca gttcgctcgg gaaacccaga gccgcgagga gcggacgcgc
     2221 cggatcgcgg tcgaggaacg gctgcgcatc gcacgcgagc tgcacgacgt cttcgggcac
     2281 accatggcca ccatcagcgt gcaggccggt gtaggtctgc acgtcatcga acaacgcccc
     2341 ggacaagccc gcgaggcact cgccgcgatc aagaagatct gcgacgacgg cctcaccgac
     2401 gtcaagacca ttctcggcat cctgcgcgcc gacacctcca cagaggagga gcccgagcaa
     2461 ccacgtgcgc cccggggcgg gctcgaccgg ctcaccgagc tgctcgacac ggccgaggcg
     2521 gcgggcctac gggtagagct gaacgtcgac ggcgacccgc ggccactgcc cgccccggtc
     2581 gatctggccg cctaccggat catccaagaa gcgctgacca acgtgctacg ccacgccgag
     2641 gctcgcaccg tgcggctggc gctgaagcac gaaccgtcgc ggctgctcat ccggatccgc
     2701 gacgacgggc ctactaccag cgcggacacc caccacaccg cggcggggca ttacaggcac
     2761 ggcatcgacg ggatgcgcga gcgtgcccgc gcgttgtccg gtcagctcac cgcaacccca
     2821 caccccgagg gcggcttcga ggtatgcgcc gagctgcctg tcccgcaccc gcgatgatgc
     2881 ccacgtgcgc ccacccgacc cgctcccgac tcacccgccc gccacggccg cgaccggcga
     2941 gaccccaggg cgctcagcgg tgaccgagcc catccgcgtg ctcatcgccg acgacgaagc
     3001 cctcgtccgg ggtggcttcc gagtactcat cgacaatgag cccggcatga tcgtggtcgg
     3061 cgaagccggc gacggagcca ccgcggtgga cctggcccgg cacacccgcc ccgacgtcgt
     3121 gctcatggac atcaggatgc ccggcgtcga cgggctgcgc gccacccacc agatcgccgc
     3181 cgaccccgac ctcgccgacg tccacgtgct catcctcacc accttcgacc acgacgagta
     3241 cgtcatcgaa gcactcggtg ccggcgccgc cggcttcctg atcaaaaaca ccgaaccccg
     3301 ccaactgctg cacggcatcc gcgtcgtcac cggcggcgag gccctcctgt ccccaggact
     3361 gacccgccgg ctgatcgccc aactcgccaa ccgaaccacg agcccacgac tcaacaccga
     3421 cgccctggct caactcacca cccgcgaaaa gcagatcgtc ggactcgtcg cccacggcct
     3481 gagcaacacc gacatcgcca ccgccctcac catcagccac gccactgcca agacccacgt
     3541 cagccgcgcc atggccaaac tcggcgcccg cgaccgcgct cagctggtcg tactggccta
     3601 ccaacaccgc ctcgtcgaac cacccgcccc caccccatag cccactccgc cctgccggtg
     3661 aggtcagctc tcccacgtcg tcgagctggg gcagacgttg aactcgtcta cggcgtcgat
     3721 cgtgcgctgt gcctccggtc gcccgaaccc gacaccggcc atcgcccggg ccaccaagtc
     3781 gatcgcctcc tcgcgggtca tgtcgcgcgc cttccgctcg caggcagcgg tggcggcggc
     3841 gtggggcccc cgaagaaacg ggattccctc gacggcggcg aggtagccgg agtaatcggg
     3901 gccgtccagc gcgtgaggcg ccagcgggcc agcggaaaca ccaccgcccc cgcacccggc
     3961 tagtagagca ccgagggcga gggcggggag caggcgccgg agatccaccc agggatactg
     4021 acagaccgcc gtgcctggtg ggcggtggtc ggcgaacggg cagcgaaata gcctgaccga
     4081 tctccccagc gttgatcaga caaagaacgg ccccccgccc agatcatcgg tcgagggccg
     4141 tggtatgtcg ggcggggccg ggattgcacc cgagggcgta caggcccacg ccggagctcg
     4201 cgagagcatc cgctgcccta ccgagccgca tcccggcctc ccgtgaccag ccagctgcac
     4261 gccccgtcgg gctcgtcgag ccacacccgg tgactcccgt cctgccggac ggtcaacccg
     4321 agccgatccc atccgggccg gccgagcatg tcccactggt cgtgggcgcg ctcgaactgc
     4381 gtccagatcc cgatcggtcc cccctcgcac acctcgcggc gcccccgctc atcgggcagc
     4441 atccgcacca tgcaccacga cccgtcacca ccgcggaacg tcgcccattc cgacccggtc
     4501 tcggtacgcc cgcggtaacc gaacaccagc cgctgcggta gccgggaact cgccaggaac
     4561 cacggcacca ccgccgtcca cggttgcggg tcgagctggg tcgacgagcg ccacccgacc
     4621 tcgggttgca tggtcacgcc ggtcagggtc ggtggggcgg cgtcggtgtc gcggatcgcc
     4681 atgaaggttg cccacttcgg caggaaccgg ccctccaacc ggtcggggta ccggcgcagc
     4741 agcgcgaggt tcccggtgtg cgcgctgcgc ttgacgtcga cgagcaccag cccgccgtcc
     4801 cggagctgct cggcccacgc ccacggcacc accggcaccg agcaggtcgc gatgatccgg
     4861 tcgaacggtg cctgatcggg cagaccgaag gcgccgtgca ccgccgccag cgtcggcgcg
     4921 tacccgaggc cggccagccg ttcccgggca gcctcgacga gcgcggcgcc gatgtcgacg
     4981 gagaacacct gcccatcgcc gagccggtgc gccagcagcc cggcgttgta cccggtgccg
     5041 gtgccgatct ccaggacacg gtgcccgagg cgcacgtcca gcgcggcaag catgcgcaac
     5101 atcaacgcgg gctgcgacga ggaggacatc gtcacccgcc caccggccgc gttctcggcg
     5161 agcgcagtca ccaacgggcg gtcgtcatac accgcgtcaa gccacccgtc ggacccggcc
     5221 gaggtcgccg accagtcccc gcctggctgc tggacgtaga acgtcggcac gaacacgtgt
     5281 cggggcaccg actcgactgc gtccctccac gccggatcgt ccagcacacc ggcctcggtg
     5341 agcttctcga cgagcccgtg ggcccgcgcc tgccagtcca cagctgtcgt catgttcaaa
     5401 cgagcccctt ctcgcgccgg tcgaccatga gcttctcgat ctcgcgagcc cactcgttaa
     5461 ccgtcggcat tccgccgccc gcgcgctgcc atccgaggga ggcggccact ccgcgcggcc
     5521 gcgtcgactc gagggcccgg agcgcgccac gaggacgccg ggcaaagcca cgatccgcgt
     5581 cgcgttttgc taacagggca cgacgcacgt acccgcccaa ccccaaaccc agtgctcaac
     5641 cggcgcccag cggactgtgc gagatcggca agaaacggcg taggggaagt tcacgtccgc
     5701 gcggacacaa ctgttggtac gcggtcactt ctcttcaggc acgtgcttgc caaacgatga
     5761 tcacgttgtt cgcgatcggg tactcgttcg gaaaacggca tcggtagtag gcgcgctggt
     5821 ggtgccaggt cccctgcatc cggcgcccgc aggcatcaca gatggccaac caccaaaggg
     5881 ctcgggaacc tgcaacattc acgccgcgcc gtgggcggtc ggctggaggc caccgttctc
     5941 ctcgtcttga ccaccgtgca ggctccgcag caccccttcg ggcggtcggt gctcggcgag
     6001 ctgcctgtcc agcggtccgg accggatcgc tcccccgccg ccgtggcggg tgatccgctg
     6061 cagcaggagg agcagcacga acccgaggac gatgagcccg tgggtccaca gccgggatgg
     6121 atcgacaccg ccgcgcagga catccagcac actcagcacc gtgagcactc cgacgaaggc
     6181 ccccaccaac gggaccagcc ccgccggccg cgcggtccct gtcgcgaccc acaggaatcc
     6241 gatggcgagt gccaggttcc acgccgagct ctcgtgcgac aggtgcgcag cacttgcccc
     6301 ggccagttcc actaccccgt ggtgggcgcc caccgccagg atgccattga tcgccagggc
     6361 gcactgaccg agacctacga cacccagcgc gaggcggact gggcccgggt gcaggcgtcg
     6421 acgcggagtc ggcggcgccg cgtcgagcac caccgcgacc agatccggac ctgattcggc
     6481 ggcccgggta cgggtcagcc gcgtaatccg ggcggcccgg tcggcatagc gggagcagtc
     6541 ggcgcacgat gcgacgtggg cgtcgatcgc ttcggactcg cctgggagat cttccccgtc
     6601 caaccgcgcc gagatcgcgt cgcgacaatc cacgcacccc atgcaggtat ggtcgcgcgt
     6661 tgcggcctcg tggttccaga gcacttgcac gatatggttc tacgcatggt cgaacgtccg
     6721 gacgacgcac gcatcaccgc gttggccttg gccgccgggc gcggggaccg cacagcgctc
     6781 gccgagttcg tccgggccac ccagcgtgac gtgcaccgtt tcgtgacgta cctcagcggc
     6841 cccggcgacg ccgacgacct ggcccaggag acctacctgc aggcgctgcg ctcgctgccg
     6901 aggttcgcga atcgttccac cgcgcgcata tggctcctct cgatcgcccg tcataccgct
     6961 gccgatgccg tacggatcgc ggtccgcagg ccccgcacca gcggcgtcga cgactgggag
     7021 gcgctcgccg cggccaccgg cagccgcgag ctggtccgct ccgccggcga cgaggcagtg
     7081 cttctgcgca gcctcgtcga ccggctcgac ctcgaccggc ggcaggcatt cgtgctgacc
     7141 caactgctgg atctgagcta tgccgacgcc gccgagatct gcggctgccc ggtcggcacc
     7201 atccggtcca gggtcgcccg ggcccgggag gacctcgtcg aagcggtggc ggacacgcgc
     7261 gggtcccgac gggcaggcca gggctgatcg gcccgcgaac acccgcggaa cgacaggttg
     7321 tcccgcttgg gctcgcgcac cccgaacacc gcctcgcgca gcgccgtggg atcgtcgccc
     7381 gggccctgca tcagcgacgg gatctcgtag acgatgtgcg ccagcgccct gacctgcggg
     7441 ctcggccggt cctccccgcg gtcggtggcg atcgcctcgg cgagggcgcg ctcgtgccgc
     7501 gcccacatcc tgccggcgta ctcccgcagg gctaggctct ccatgaccag ctgccggaag
     7561 cgcaggtggc ccgattcctt catcgcctcg gtgacggctg cactgttccc gagcgtgaag
     7621 gcatgcagcg cagcgagcag cgggaccgcc tggtcgcggt cgcgaacggc cgcgaccagg
     7681 gcctcctcct ggtcggcggc gcggtcgaag aacagcgcct tcttgctggg gaagtgctct
     7741 tgaacgcgtc gcgctctgct gctcagcgag caggcgttcg cgcagcatgt agcgctggat
     7801 ctcggtgctg gacgtggggc accgcggcac ccggtaggag gcgatccagc gcggcagtga
     7861 accagctcgt cggcgagcgg ccccgggaac cggtgacgcg gcggcgggcg cgccgggagc
     7921 agcggtgtca ctcgctgcca tagcgagtcc ggaacaagat cctcgatcac gcgacgcggc
     7981 ctggcgaagg tcagtctccc tctacagcag acacgccggc ggccccggcg
//
LOCUS       DASRSC010000101         3233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3144745,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000101 DASRSC010000000
VERSION     DASRSC010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3233
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3144745"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VFE39_04875"
     CDS             <1..160
                     /locus_tag="VFE39_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6257967.1"
                     /translation="HCHYGYNAKGGPAGVGIAVGRAVRLSIVSTAILDFFLTLVIYGT
                     STSVSVAG"
     gene            192..1508
                     /locus_tag="VFE39_04880"
     CDS             192..1508
                     /locus_tag="VFE39_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HET6257968.1"
                     /translation="MKRRLQGLAFVVVLLAMAGVAVGDYAGFFSNGVPVTLKVDRVGT
                     QLAPRADVKVRGLNVGTVTSVSTDGSGATVQLSLDRDKIAQIPGGVSAQLLPKTLFGE
                     RYVSLVPPSGPPGPPLAAGDVIPEDRSAAAREVERVLDGLLPLLQAVAPQDLATTLGA
                     LSQALSGRGEDLGKTLVQLQKLTGGLRPAVPDLQADITQLADFAHNVNQAAPDLLDAL
                     QNFSVTSRTIVEQRQQLNDLLTGVTQASDDLNAFLMENRENLIGLAASSRPTLESLAR
                     YAPEFPCLTGQLAGLVPRVDQAFGVGTDHPGLHVTLEIVVNRGKYVPHQDEPRYLDDR
                     GPRCYPIQPLGPQQPPDGPLKDGSKPTPAPVGTPKGSAEDFGAIPAAYSGTGGYAGMG
                     VPNSPAEQQVVAELTAARDGGSPAAVPNWSTMLVGPLYRGTEVTLT"
     gene            1505..2653
                     /locus_tag="VFE39_04885"
     CDS             1505..2653
                     /locus_tag="VFE39_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HET6257969.1"
                     /translation="MNRIPLAPLIKFTVLTVIVALATTVLALTIANASPGEHTSYSAR
                     FTDASGLLPGDDVRIAGVVVGTVDNIRIVDRRVAEVAFSVSRDQRIPASVTAAILYKN
                     LIGQRFLSLALGTGPTGETLPPGGTIPVERTRPPLNLTTLFNGFKPLFAALDPQQVNQ
                     LSLEIIQVLQGEGGTVQSLLASTSSLTNTLADRDAVIGQVIDNLNAVLDTVNSRDKEL
                     SDLISSLQALVSGLADDRKPIGDAIVSIGKLTTVTSGFVEEGRPALQKDIAALGDLAG
                     NLNDSAPKLEQTLLNLPGKLETVSRAGSYGSWFQFYLCGVTGSVGMEPYLPAAEIPAF
                     SSASPRCGPDPSGVQGQSPKALGGIPPVAPDGSGPPGLPPALSLPGVR"
     gene            2653..>3233
                     /locus_tag="VFE39_04890"
     CDS             2653..>3233
                     /locus_tag="VFE39_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HET6257970.1"
                     /translation="MPGQHNPVRTAVIGLLVIVLVVVLAFNAQALFGGGTTYRAEFSE
                     AAGLTSGDLVTIAGVEAGRVGSVVLAGDRVLVTFTVSDAWVGDQTSASIEIKTLLGAK
                     YLALDPQGDNALKPGAVIPLARTASPFDVVEAFNGLSGTIDQLNTKQLAQSLTTLADT
                     FRGTAPEVRGALDGLSRLSTTIASRDDEIKKLLA"
BASE COUNT          479 a         1171 c         1116 g          467 t
ORIGIN      
        1 ccattgtcac tacgggtaca acgccaaggg cgggcccgcg ggggtcggca tcgcggtcgg
       61 gcgagcggtg cgcctgtcga tcgtcagcac cgcgatcctc gacttcttcc tcacgctggt
      121 catctacggc acctcgacgt cggtgagcgt ggccggatga cggcctcgga gtcgctcggc
      181 ggaggcgcgt cgtgaagcgc aggttgcagg ggctcgcctt cgtcgtggtg ctgctcgcga
      241 tggccggggt cgccgtcggc gactacgccg ggttcttctc gaacggcgtg ccggtcacgc
      301 tgaaggtcga ccgggtgggc acccagctcg ccccgcgcgc agacgtgaag gtccgcggtc
      361 tgaacgtcgg caccgtcacg tcggtgagca cggacgggtc cggcgccacg gtgcagctct
      421 ccctcgaccg ggacaagatc gcgcagatcc cgggcggcgt ctccgcccag ctgctcccca
      481 agaccctgtt cggggagagg tacgtgtcac tggtgccgcc cagcgggccg ccgggtccgc
      541 cgctggctgc gggggacgtg atcccggagg accgcagcgc ggccgcgcgc gaggtggaac
      601 gggtgctcga cgggctgctc ccgctcctgc aggccgtggc gccgcaggac ctcgcgacca
      661 cgctcggcgc gctgtcgcag gccctgtccg ggcgcggtga ggacctcggt aagaccctgg
      721 tgcagctgca gaagctgacg ggcggcctgc gtcccgcggt ccccgacctc caggcggaca
      781 tcacgcagct ggccgacttc gcccacaacg tgaaccaggc ggcccccgat ctgctcgacg
      841 cgctgcagaa cttcagtgtg accagccgga cgatcgtgga gcagcgccag cagctgaacg
      901 acctcctcac cggagtcacc caggcgtcgg acgacctgaa tgcgttcctc atggagaacc
      961 gcgagaacct gatcgggctg gccgcatcga gccgccccac gctggagagc ctggcgcgct
     1021 acgcgccgga gttcccgtgc ttgaccggcc agctcgccgg gcttgtgccg cgcgtcgacc
     1081 aggcgttcgg cgtgggcacc gaccacccgg ggctccacgt cacgctcgag atcgttgtca
     1141 acaggggcaa gtacgtgccc caccaggacg aaccgcgcta cctcgacgac cgcgggccgc
     1201 gctgttaccc catccagccg ctgggcccgc agcagccgcc agacgggccg ttgaaggacg
     1261 ggtcgaagcc gacacccgcg cctgtcggca caccgaaggg cagtgccgag gacttcgggg
     1321 cgatccccgc cgcgtactcg ggtacgggcg gttacgccgg catgggcgtg cccaactcgc
     1381 cggccgagca gcaggtcgtg gccgagctca ccgctgcccg cgacggcggc tcgcccgctg
     1441 cggtgccgaa ctggagcacg atgctggtcg gcccgctcta ccgcgggacc gaggtgaccc
     1501 tgacgtgaac cggatcccgc tcgcgccact gatcaagttc acggtgctga cggtcatcgt
     1561 cgcgctggcc accaccgtgc tcgcgctgac gatcgcgaac gcctcgccgg gcgagcacac
     1621 gtcctactcc gcccgcttca ccgacgcctc cgggctgctc cccggggacg acgtgcggat
     1681 cgcgggggtg gtcgtcggca cagtggacaa catccggatc gtcgaccggc gggttgccga
     1741 ggtggccttc agcgtctcgc gcgaccagcg gatcccggcg tcggtgaccg cggcgatcct
     1801 ctacaagaac ctcatcggcc agcgcttcct gtcactcgcg ctgggcaccg ggcccaccgg
     1861 ggagaccctc ccgccgggcg gcacgatccc ggtggagcgc acccgcccgc cgctgaacct
     1921 caccaccctg ttcaacgggt tcaagccgct cttcgcggcg ctcgacccgc agcaggtcaa
     1981 ccagctgtcc ctcgagatca tccaggtcct gcagggggag ggcggcacgg tgcagagcct
     2041 gctcgccagc acgtcgtcgc tgaccaacac ccttgccgac cgcgacgcgg tgatcgggca
     2101 ggtcatcgac aacctgaacg cggtgctcga cacggtcaac tcccgcgaca aggagctgtc
     2161 ggacctgatc agctccctgc aggcgctcgt ctccggcctc gccgatgacc gcaagccgat
     2221 cggggacgcg atcgtctcga tcggcaagct cacgacggtc acctccggct tcgtggagga
     2281 ggggcggccg gccctgcaaa aggacatcgc cgcgctgggc gacctcgcag gcaacctgaa
     2341 cgactcggcg ccgaagctgg agcagacctt gctgaacctg cccgggaagc tggagacggt
     2401 gtcccgggcg ggcagctacg gcagctggtt ccagttctac ctgtgcggtg tcaccggttc
     2461 cgtcgggatg gagccctacc tccccgcagc cgagatcccc gcgttcagca gcgcttcccc
     2521 gcgatgtggg ccggacccca gcggcgtgca gggccagagt cccaaagcgc tcggcgggat
     2581 cccgccggtc gcccccgacg ggtcggggcc accggggctg ccacctgcac tgtcgctgcc
     2641 gggagtgcgc tgatgcccgg acagcacaac ccggtccgga ccgcggtgat cggcctgctg
     2701 gtgatcgtgc tcgtcgtcgt gctggcgttc aacgcgcagg cgctgttcgg cggtggcacc
     2761 acctaccgcg ccgagttcag cgaggcggcc gggctcacct ccggcgacct cgtgaccatc
     2821 gcgggcgtcg aggccgggcg cgtcggctcc gtcgtgctgg ccggcgaccg ggttctcgtc
     2881 accttcacgg tttccgacgc ctgggtgggc gaccagacct ccgcctcgat cgagatcaag
     2941 acgctgctcg gcgcgaagta cctggcactg gacccgcagg gcgacaacgc tctaaagccc
     3001 ggtgcggtga tcccactggc gcgcactgcg tccccgttcg acgtggtgga ggcgttcaac
     3061 gggctctccg gcacgatcga ccagctgaac accaagcagc tggcgcagag cctgaccacg
     3121 ctggcggaca ccttccgggg aacggcgccg gaggtccggg gtgcgctgga cgggctgtcc
     3181 cgcctctcca ccacgatcgc gagccgggac gacgagatca agaaactgct tgc
//
LOCUS       DASRSC010000102        16421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3309178,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000102 DASRSC010000000
VERSION     DASRSC010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 16421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16421
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3309178"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..3356
                     /gene="carB"
                     /locus_tag="VFE39_04895"
     CDS             69..3356
                     /gene="carB"
                     /locus_tag="VFE39_04895"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET6257971.1"
                     /translation="MPKRDDIRHVMVIGSGPIVIGQACEFDYSGTQACRVLQAEGIRV
                     SLVNSNPATIMTDPEFADATYVEPITPEFVEQVIAAERPDAILATLGGQTALNTAVAL
                     HENGVLERYGVELIGADIDAIQRGEDRLKFKEIVQSVGGDVPRSAVCHSMDEVRAAVV
                     ELGLPVVIRPSFTMGGLGSGMAHTTADLERLAGGGLAASPVAEVLIEESVLGWKEYEL
                     ELMRDHHDNVVVVCSIENLDPMGVHTGDSITVAPAMTLTDREYQHMRDVGIDVLRAVG
                     VDTGGCNIQFAVHPATGRLVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGYTLDE
                     IRNDITGETPAAFEPTLDYVVVKIPRFAFEKFPGADPQLTTTMKSVGEAMALGRSFPE
                     ALGKALRSMETTAAGFWTTPDPDGEPDVGTALDGRIYAVERALRGGATVADVAQRSGI
                     DPWFLDQILALTELRQEILDTPVLDEPLLRRAKRAGLSDRQLAALRPEFAGEDGVRTL
                     RHRLGIRPVYKTVDTCAAEFAARTPYHYSAYETDPAAESEIAPQTEKPKVLILGSGPN
                     RIGQGIEFDYSCVHAVMALREAGFETVMVNCNPETVSTDYDTADRLYFEPLTFEDVLE
                     VVHAERQSGTVAGVLVQLGGQTPLGLAQRLTAAGVPVVGTSAAAIDLAEDRGAFGEVL
                     RKAGLPAPAFGMATSFDQAREIAERIGYPVLVRPSYVLGGRGMEIVYDDETLAGYISR
                     ATEISPERPVLVDRFLDDAIEIDVDALCDGEEVFLGGVMEHIEEAGVHSGDSSCTLPP
                     ITLGRSVLEQVRRSTEAIAHGVGVRGLLNVQYALHGETLYVLEANPRASRTVPFVSKA
                     TAVPLAKAAARIMLGASIAELREEGLLPAEGDGGELPPDAPIAVKEAVLPFHRFRTRD
                     GQGIDSLLGPEMKSTGEVMGFDASFGAAFAKSQAAAYGSLPTSGRVFVSMADRDKRAM
                     VFPVLRLAALGFEVLATAGTADVLRRHGVSTTVVRKHFEGGETIVDRILAGDVDMIIN
                     TPYGNSGPRVDGYEIRAAAVSRGIPCVTTVQGAAAAVQGIEAMIGGSIGVRSLQDAHA
                     ALRKSRP"
     gene            3362..4189
                     /gene="pyrF"
                     /locus_tag="VFE39_04900"
     CDS             3362..4189
                     /gene="pyrF"
                     /locus_tag="VFE39_04900"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET6257972.1"
                     /translation="MVRATFGGRLDAAVSRFGALCVGIDPHPGLLAEWGLTDDPDGLE
                     RFAMSCVEALVGHVAVVKPQSAFFERHGSRGIAVLERVLAAFAGGPTLTLLDVKRGDI
                     GSTMDAYADAYLAIGAPLGADAVTLSPYLGFGSLGPALDAAVDAGRGAFVLARTSNPE
                     GAPVQLAEWAGRSVGQTMVDAAAARNTGAEPLGDVGVVVGATSDHGLDLSALGGPVLA
                     PGLGAQGAGPADIVARFAGVSGIVLPSASRSVLTAGPDAAAVRSAAEALRDELAAEH"
     gene            4531..4845
                     /gene="mihF"
                     /locus_tag="VFE39_04905"
     CDS             4531..4845
                     /gene="mihF"
                     /locus_tag="VFE39_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003872557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HET6257973.1"
                     /translation="MALPQLTEEQRAAALEKAAAARRARAELKDRLKRGGTTLPEVLK
                     QSDTDEVLGKMKVSALLEAMPGVGKVRAAQIMERLEIAPSRRLRGLGDRQRKALLADF
                     ES"
     gene            4866..5504
                     /gene="gmk"
                     /locus_tag="VFE39_04910"
     CDS             4866..5504
                     /gene="gmk"
                     /locus_tag="VFE39_04910"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET6257974.1"
                     /translation="MSSSTPGPPGRRGRLVVLAGPSGVGKSSVVTALRAQLPSLFFSV
                     SATTRNPRAGEVDGVHYRFVGPAGFDGLIERGALLEWAEIHGGLQRSGTPREPVEQAL
                     DAGRPVLVEVDLQGARAIKAVLPDAITVFLEPPSFDELARRLRERGTESQEQYERRLE
                     TAREELAARSEFDVVVVNDELKDVAARLVQLLVGPLLRDTVPTPDHPQESSS"
     gene            5507..5782
                     /gene="rpoZ"
                     /locus_tag="VFE39_04915"
     CDS             5507..5782
                     /gene="rpoZ"
                     /locus_tag="VFE39_04915"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET6257975.1"
                     /translation="MPLTGAVAGSTPEGITNPPIDDLLGQVSSKYALVIYAAKRARQI
                     NDYYSQLGEGLLEYVGPLVEPGPREKPLSIAMREIQAGLLEHTEGEQ"
     gene            5814..7034
                     /gene="coaBC"
                     /locus_tag="VFE39_04920"
     CDS             5814..7034
                     /gene="coaBC"
                     /locus_tag="VFE39_04920"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HET6257976.1"
                     /translation="MDSLTPPRVLLGVAGGIAAYKSAELLRRLTETGHSVRVLPTASA
                     LEFVGAATFEALSGQPVHTGVFVDVPSVPHVKLGQGADLVVVAPATADLLARAAAGRA
                     DDLLTASLLTARCPVLFAPAMHTEMWEHPATRANVELLRSRGVVVLEPASGRLTGKDT
                     GPGRLPDPAEIAEFARLLLERTDALPHDLSGRRVVVSAGGTREALDPVRFLGNRSSGR
                     QGYALARVAAQRGASVTLVAAHTADLGDPAGVEVVRVGSAAQLRDAMYAAARGAHALV
                     MAAAVADFRPAALAEHKIKKTSGDPEPLVLTTNPDILAELVTTKEPGQVVVGFAAETG
                     DAAGDVLHHGRAKLLRKGCDLLVVNAVGDGKAFEVPDNSGWLLGADGSETELKPGSKA
                     LLATRVWDAVASRL"
     gene            complement(7112..7510)
                     /locus_tag="VFE39_04925"
     CDS             complement(7112..7510)
                     /locus_tag="VFE39_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HET6257977.1"
                     /translation="MKASTSLSYRPGRAAAVVGAMVAVGATGLMALVGGTAAASEPGR
                     CTENINVRSGPDAHAPIVAQCEAGSGVRVGEKRQGFVQLIDLGGWASTQYVAADGAAP
                     VRPVLRAPEGVAPTPAPQPPGARPAGGPFG"
     gene            7768..8970
                     /gene="metK"
                     /locus_tag="VFE39_04930"
     CDS             7768..8970
                     /gene="metK"
                     /locus_tag="VFE39_04930"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET6257978.1"
                     /translation="MANAGRRLFTSESVTEGHPDKMCDAISDTVLDALLAQDARSRVA
                     VETLVTTGQVHIAGEVTTSAYADIPTLVREKILDIGYDSSAKGFDGASCGVNVAIGAQ
                     SADIAQGVDTAYESRVELSEDEIARQGAGDQGLMFGYANTDTPELLPLPIALAHRLAR
                     RLTDVRKSGVLPYLRPDGKTQVTIEYDGDKAVRLDTVVVSSQHAADIDLDGMLAPDIR
                     EHVVDPEISKLGLDVADLRLLVNPTGRFVVGGPMGDAGLTGRKIIVDTYGGFARHGGG
                     AFSGKDPSKVDRSGAYAMRWVAKNAVAAGLAERIEVQVAYAIGKAAPVGLFVETFGTE
                     HVDPLKIQSAITEVFDLRPAAIIRDLDLLRPIYAQTAAYGHFGRTDVELPWECTSRAD
                     ALKAAAGG"
     gene            9201..11306
                     /locus_tag="VFE39_04935"
     CDS             9201..11306
                     /locus_tag="VFE39_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET6257979.1"
                     /translation="MVRPGRNRGEWQAAAELPVARVAVDVALAHLDRPFDYRVPEHLD
                     AVAVPGVRLRVRFAGRLVDGFLLERLAESAHPGTLAWVEKVVSPEPVLTAEVAALCRA
                     VADRYAGVLADVLRLAVPPRHAKAEAEVPPQRAGPVAAPGEPGAWRRYPRGPALLDAL
                     AGGRSAHAVWQALPGECWPERLAEAAAATVAAGRGALLVVPDQRDVAALHGACAGLLG
                     ADAVVALTAELGPAERYRRWLAVRRGHVQVVVGTRSAAFAPVERLGLLAVWDDGDDLH
                     AEPRAPYPRVRDVLVLRAHAAGAALLVGGYARTAEAHLLVESGWAREVIAERATVRAV
                     MPRVTALGETDGQLARDPDSRAARLPHLAFEAARSALTAGRPVLVQVPRSGYLPWLAC
                     GTCRETARCRHCAGPLGLTGHGTRGRSGGAVFGPTPHASGTPPHAGSAGLPHCRWCGR
                     ADSAFRCPACGSRRLRAGVVGSGRTAEELGRAFPGTTVRSSGGGAPVLAEVAARPELV
                     VATPGAEPRAAGGYGAALLLDGWALLSRPDLRVAEETLRRWLGAAALVVPHTDGGRVV
                     VMADAAIPVVQSLVRWDPAGHAAAEFASRAEVGFPPAVRMAAVEGTAAAVADVLAELP
                     EVVEVLGPVELDPVGTGSGSFANTADEPPEIRERALLRVPRADGRTMAAALHAAQAVR
                     TARKESDPVRVRLDPVEIG"
     gene            11440..11988
                     /gene="def"
                     /locus_tag="VFE39_04940"
     CDS             11440..11988
                     /gene="def"
                     /locus_tag="VFE39_04940"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET6257980.1"
                     /translation="MSVQPIRFFGDPVLRTPAVEVTTFDAELRKLVSDLTETMHDEGG
                     AGLAAPQLGVGLRVFTYDCEGFAGHLVNPTFEVVGDEEQLGPEGCLSIPGLSWDCRRH
                     LHVVAHGWNQHGEPVTVEGSEKLARCIQHEIDHLDGVLFVDRLDSETRKQAMAEIRAA
                     EWFGGSEPRVQVSPHPLFGKAR"
     gene            11995..12945
                     /gene="fmt"
                     /locus_tag="VFE39_04945"
     CDS             11995..12945
                     /gene="fmt"
                     /locus_tag="VFE39_04945"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET6257981.1"
                     /translation="MKLVFAGTPEPAVPSLRALLGSPRHEVAAVVTRPDAPAGRGRKI
                     VRSPVGTLADEAGIPVLTPARPSDPAFLAQLAELAPDCCPVVAYGALVPRAALDIPRH
                     GWVNLHFSLLPAWRGAAPVQAAVRHGDEVTGATTFLLEAGLDTGPTFGVVTEAVGPQD
                     TAGDLLDRLAVSGAGLLLATMDGIADGTLRPRAQPSDGVSHAPKVTTADARVDWAAPP
                     LAVDRLIRSVTPEPGAWTTFRADRLGLGPVGATTDAPGGDAGLPVLKPGELHVEKRRV
                     LVGTAAAPLELGEVQPVGKRAMPAPDWARGVRIEPGELLT"
     gene            13139..14485
                     /locus_tag="VFE39_04950"
     CDS             13139..14485
                     /locus_tag="VFE39_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET6257982.1"
                     /translation="MDPSRQAALDLLTAVRVRDAYANLALPSILRRYRLHDRDAALAT
                     ELGYGTLRARGLLDAVIEACTDRPLIRVEPALLDALRLGAYQLLRTRIPAHAAVDTTV
                     ELVRGDAGSRAAGFVNAILRKVGERDEQAWVERLAPPADEDPVGHAAMAHAHPRWIAQ
                     SFADALGAPEELDAALAADDARPTVHLLARPGEITAAELALVTGGEEAPYSPYGVHLE
                     PGGGDIAEIDAVAEGLAVVQDEGSQLMALALSRAALAGADTGRWLDLCAGPGGKAALL
                     GGIAALDGASVDAVEVSEHRADLVRRATDGLPVTVHKADGRAAPLPEGTYDRVLVDAP
                     CTGLGALRRRPEARWRRRPEDVSGLTKLQRELLTAALRHVRPGGVVAYVTCSPHLAET
                     VGVLTGVLRRHPDVEQLDAREYLPDMPDLGAGPTVQLWPHRHGTDAMFLALLRRTA"
     gene            complement(14492..14893)
                     /locus_tag="VFE39_04955"
     CDS             complement(14492..14893)
                     /locus_tag="VFE39_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HET6257983.1"
                     /translation="MPAPVSIDEQLDHLLVGELPAVAVLVRRLRAVVRIAHPDLRERV
                     YPGWHGLGFHHPRGGYVCALFPRDGGVNVGFEHGADLPDPHGRLLGEGRRMRDVRIEV
                     DAGPDEEELVVEYLDLAVDWALDRASGTRGR"
     gene            14980..15666
                     /gene="rpe"
                     /locus_tag="VFE39_04960"
     CDS             14980..15666
                     /gene="rpe"
                     /locus_tag="VFE39_04960"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET6257984.1"
                     /translation="MIAPSILAADFARLADEAAAVAGEPGHGADWLHVDVMDAHFVPN
                     LTIGLPVVESLIRATTIPLDCHLMIDDPDRWAVGYAEAGAHNVTVHVEAARDPVGLAA
                     DLRAAGARAGLSVKPGTPLEPYVETLRHYDTLLVMSVEPGFGGQSFMPEVLDKVRAAR
                     RMVDTGHLTLLVEIDGGINAETIEAAAEAGVDCFVAGSAVYSADDPARAVQALREQVL
                     GASAHRRWNQ"
     gene            15773..>16421
                     /locus_tag="VFE39_04965"
     CDS             15773..>16421
                     /locus_tag="VFE39_04965"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET6257985.1"
                     /translation="MFTGIVEEIGEVLAVRESGEVVVLTVRGRTVTADAGHGDSIAVN
                     GVCLTVIDPAGSSDGTFTVELVPETLKRSSLSAVAEGARVNLERAVPVTGRLGGHIVQ
                     GHVDGVATLLSRTPGERSDELRFSLAPDLARYVVEKGSIAVDGVSLTVAAVTPDGFTV
                     ALIPTTLSHTTLGLRQVGDTVNLEVDVVAKYVERLAAGYTGRTGITATVGEEARAQ"
BASE COUNT         2185 a         5791 c         6171 g         2274 t
ORIGIN      
        1 caccccgccg acactccgct gggcactgat ccgcccttcg ctgacgctcc acacggggaa
       61 ggcgcctgat gcccaagcgc gacgacatcc ggcacgtgat ggtgatcggc tccggaccga
      121 tcgtcatcgg ccaggcgtgc gagttcgact actccggcac ccaggcctgc cgcgtgttgc
      181 aggccgaggg catccgcgtc tcgctggtga actccaaccc cgccacgatc atgaccgacc
      241 ccgagttcgc cgacgccacc tacgtcgagc cgatcacccc ggagttcgtg gagcaggtca
      301 tcgcggccga gcggccggac gcgatcctcg cgacgctggg cgggcagacc gcgctgaaca
      361 ctgcggtcgc gctccacgag aacggcgtgc tggagcgcta cggcgtcgag ctcatcggcg
      421 ccgacatcga cgcgatccag cgcggtgagg accggctgaa gttcaaggag atcgtgcagt
      481 cggtcggcgg cgacgtgcct cgctccgccg tctgccactc gatggacgag gtgcgcgccg
      541 cggtagtcga gctgggtctc ccggtcgtca tccggccgtc gttcacgatg ggcgggctcg
      601 gctccggcat ggcccacacc acggccgacc tcgaacggct cgccggcggt gggctcgccg
      661 cgtcgcccgt cgccgaggtg ctcatcgagg agtcggtgct cggatggaag gagtacgagc
      721 tcgagctgat gcgcgaccac cacgacaacg tggtggtggt ctgctcgatc gaaaacctcg
      781 acccgatggg cgtgcacacc ggcgactcga tcacggtcgc ccccgccatg accctcaccg
      841 accgtgagta ccagcacatg cgcgacgtgg gcatcgacgt gctgcgggcg gtcggcgtcg
      901 acaccggcgg ctgcaacatc cagttcgcgg tccaccccgc caccggtcgc ctcgtcgtga
      961 tcgagatgaa cccgcgtgtc tcccggtcgt cggcgctggc gtccaaggcc accggtttcc
     1021 cgatcgccaa gatcgcggcg aagctggcgg tcggctacac gctcgacgag atccgcaacg
     1081 acatcaccgg tgagacgccc gcggccttcg agcccacgct cgactacgtc gtggtgaaga
     1141 tcccgcggtt cgcgttcgag aagttcccgg gggccgaccc gcagctgacg acgacgatga
     1201 agagcgtcgg cgaggcgatg gcgctgggcc ggtcgttccc cgaagccctg ggcaaggcgc
     1261 tgcgctcgat ggagacgacg gcggccgggt tctggaccac accggatccg gacggggagc
     1321 ccgacgtcgg caccgcgctc gacggccgga tctacgcggt cgaacgggcc ctgcgcggcg
     1381 gggcgaccgt ggcggacgtg gcgcagcgct ccggcatcga cccgtggttc ctcgaccaga
     1441 tcctcgcgct caccgagctc cgtcaggaga tcctcgacac cccggtgctc gacgagccgc
     1501 tgctgcgccg ggccaagcgc gcggggctct cggaccgcca gctcgccgcg ctgcgcccgg
     1561 agttcgccgg cgaggacggg gtgcgcaccc tgcgccaccg gctggggatc cgcccggtct
     1621 acaagaccgt cgacacctgc gccgcggagt tcgcggcgcg cacgccctac cactacagcg
     1681 cctatgagac cgatcccgcc gccgagtcgg agatcgcgcc ccagaccgag aagccgaagg
     1741 tgctcatcct cggatcgggg ccgaaccgga tcgggcaggg catcgagttc gactactcct
     1801 gcgtgcacgc ggtgatggcg ttgcgggagg ccgggttcga gaccgtcatg gtcaactgca
     1861 acccggagac ggtgtccacc gactacgaca cggccgaccg gctgtacttc gagccgctga
     1921 ccttcgagga cgtactcgag gttgtccacg ccgagcggca gagcggcacc gtcgcgggtg
     1981 tactcgtgca gctgggcggc cagacgccgc tcgggctggc gcagcggctg acggccgcag
     2041 gcgtgccggt cgtcgggacg tccgcagcgg cgatcgacct ggccgaggac cgtggcgcgt
     2101 tcggggaggt cctgcgcaag gcgggcctcc cggcgccggc gttcggcatg gccacctcgt
     2161 tcgaccaggc tcgggagatc gccgagcgga tcggctaccc ggtgctcgtg cggccgtcgt
     2221 acgttctcgg cggtcgcggc atggagatcg tctacgacga cgagacgctc gccgggtaca
     2281 tctcgcgtgc caccgagatc agccccgagc gcccggtgct cgtcgaccgg ttcctcgacg
     2341 atgcgatcga gatcgacgtc gacgcgctgt gcgacggcga agaggtattc ctcggcgggg
     2401 tgatggagca catcgaggag gcgggcgtgc actccggcga ctcctcctgc accctgccgc
     2461 cgatcacgct gggccgctcg gtgctggagc aggtgcgccg atcgaccgag gcgatcgcgc
     2521 acggcgtggg tgtgcgcggg ctgctcaacg tccagtacgc gctgcacggc gagaccctct
     2581 acgtgctgga ggccaacccg cgcgcgtccc ggaccgtgcc gttcgtgtcg aaggcgaccg
     2641 ccgtgccgct ggccaaggcg gctgcccgga tcatgctcgg tgcgtcgatc gcagagctcc
     2701 gcgaggaggg cctgctcccg gctgagggcg acggtggcga gctgccgccg gacgcgccga
     2761 tcgccgtgaa ggaggcggtg ctcccgttcc accggttccg cacccgggac ggccagggca
     2821 tcgactcgct gctcggcccg gagatgaagt cgaccggcga ggtgatgggc ttcgacgcct
     2881 ccttcggggc ggcgttcgcc aagtcgcagg cagcggcgta cggctcgctg ccgacgtcgg
     2941 ggcgggtgtt cgtctccatg gccgaccggg acaagcgcgc gatggtgttc ccggtgctgc
     3001 ggctggccgc cctcggtttc gaggtgctgg ccaccgcagg cacggccgac gtgctgcgcc
     3061 gccacggtgt gtccaccacg gtggtgcgca agcacttcga aggcggggag accatcgtcg
     3121 accggatcct cgcgggagac gtcgacatga tcatcaacac gccctacggt aattccgggc
     3181 cgcgggtgga cggctacgag atccgcgctg ccgcggtgtc gcgcggcatc ccgtgcgtca
     3241 ccaccgtgca gggcgccgcg gcggcggtgc agggcatcga ggcgatgatc ggtggctcga
     3301 tcggggtgcg gtcgctgcag gacgctcacg cggcgctacg gaagtcccgc ccgtgacgag
     3361 cgtggtccgc gccacgttcg gggggcgcct ggatgcggcg gtctcccggt tcggagcgct
     3421 gtgcgtgggg atcgatccgc atccggggct gctggccgaa tgggggctca ccgacgaccc
     3481 ggacgggctg gagcggttcg cgatgtcctg cgtcgaggcg ctggtcgggc acgtggccgt
     3541 ggtgaagccc cagtccgcgt tcttcgagcg gcacgggtcg cgcgggatcg cggtgctgga
     3601 gcgggtgctc gcggcgttcg ccggcggtcc gacgctcacc ctgctcgacg tgaagcgtgg
     3661 ggacatcggg tcgacgatgg acgcgtacgc cgatgcctat ctcgccatcg gcgcgccgct
     3721 gggcgccgac gccgtgacgt tgtcgccgta tctcgggttc ggctcgctgg gcccggccct
     3781 cgacgccgcc gtcgacgcag ggcgcggcgc cttcgtgctg gctcgcacct ccaaccccga
     3841 gggcgctccg gtgcagctcg ccgagtgggc gggccgcagc gtcgggcaga cgatggtcga
     3901 cgcagctgcc gcccgcaata ctggcgccga gccgctcggg gacgttggcg tggtggtggg
     3961 ggcgacgtcc gaccacggcc tcgacctgtc cgcgctgggc ggccctgtgc tcgccccggg
     4021 cctgggcgcc cagggggccg gtcccgcgga catcgtggcc cgcttcgcag gggtgagcgg
     4081 gatcgtgctg ccctcggcgt ctcggtcggt gctcacggcc ggaccggatg cggcagcggt
     4141 ccggtcggcg gccgaggcgc tccgcgacga gctggcagca gagcactgag ggcccctgcg
     4201 gggagagggc gaacggccgc tcgcagcctc ctgcgttcct gtgggcggaa agaacggctg
     4261 ccggcaacga ttcttcagta tgaaggcgaa gcggtgctcg tcaggggacg gtccttcctg
     4321 tgcgctgaac gggcggttcg tatacacgat ccggcagttt cgcgtggtat cactcatcaa
     4381 ggggcgtccg ccaaagaacc tccaggtggc acccccgatt ccccttccgg gggaggcgtg
     4441 agtacgttcg gtgatagccg cggacacgac cgcgacggtg gtgacgatgc gtcctgattc
     4501 caggccgcac gacgaaagac ggaggagacc gtggcccttc cccagctgac cgaggaacag
     4561 cgtgctgccg cgttggagaa ggcggctgcg gcgcgccggg cccgggccga gctgaaggac
     4621 cggctcaagc gcggcggaac caccctcccg gaggttctca agcagtccga caccgatgag
     4681 gtgttgggca agatgaaggt ctcggcactg ctcgaggcga tgccaggcgt cggcaaggtc
     4741 cgggcggctc agatcatgga gcggctcgag atcgcgccgt cgcggcgact gcgtggcctc
     4801 ggcgaccggc agcgcaaggc gctgctggcc gatttcgagt cctgagcgag ctttgcgagc
     4861 tcaggatcag tagcagcaca ccgggcccgc ccgggcggag gggccggctg gtcgtactgg
     4921 ccggtccctc cggcgtcggg aagagcagcg tggtcacggc gctccgtgcg cagctgccct
     4981 cgctgttctt cagtgtctcc gcaaccaccc ggaacccacg cgccggagag gtcgacggcg
     5041 tgcattaccg cttcgtgggc ccggcgggat tcgacgggct gatcgagcgc ggtgcgctcc
     5101 tcgagtgggc cgagattcat ggtggcctcc agcgctccgg cacgccgcgc gagcctgtgg
     5161 agcaggcgct ggacgccggc cgtccggtgc tcgtcgaggt cgacctgcag ggagccaggg
     5221 cgatcaaggc ggtgctgccg gacgccatca ccgtcttctt ggagccgccg tcgttcgatg
     5281 agctggcccg acggctgcgc gagcgaggca ccgagtcgca ggagcagtac gagcgcaggc
     5341 tcgagaccgc gcgcgaggag ttggccgccc gcagcgagtt cgacgtggtc gtggtcaacg
     5401 acgagctgaa ggacgtcgcg gcgcggttgg tacagttgtt ggtcggcccg cttctccgtg
     5461 acacggtccc gacgcccgac catccccagg agtcttcttc gtgagcatgc ctctgaccgg
     5521 tgctgtcgcc ggttccacgc ccgagggcat caccaacccg cccatcgacg acctgctggg
     5581 ccaggtgagt tccaagtacg cgctggtgat ctacgctgcc aagcgcgccc gccagatcaa
     5641 cgactactac tcccagctcg gggagggcct gctcgagtac gtcggcccgc tcgtcgagcc
     5701 gggaccgcgc gagaagccgc tgtcgatcgc catgcgcgag atccaggccg gcctgctcga
     5761 gcacaccgag ggcgagcagt aacacgccga gcgagcccgc gaacgaggtc gccatggact
     5821 ccctgacgcc gccccgggtg ctgctcgggg tggccggcgg tatcgcggcc tacaagagcg
     5881 cggagctgct gcgccggctc accgagacag gccactccgt gcgggtgctc cccacggcct
     5941 cggccctgga gttcgtcgga gccgccacgt tcgaggcgct gtccggccag ccggtgcaca
     6001 ccggcgtgtt cgtcgacgtg ccgtcggtgc cgcacgtgaa gctgggtcag ggggccgacc
     6061 tcgtcgtcgt cgcgcccgcc accgccgacc ttttggcccg cgcggccgcc ggtcgtgcgg
     6121 atgacctcct caccgcgtcg ctgctcaccg cccggtgccc ggtgctgttc gccccggcga
     6181 tgcacaccga gatgtgggag caccccgcga cgcgcgccaa cgtcgagctg ctgcgctccc
     6241 ggggcgtcgt cgtcctggag cccgcgtccg gccggctgac cggcaaggac accggcccgg
     6301 gccggctgcc cgaccctgcc gagatcgccg agttcgcccg cctgctgctc gaacgcaccg
     6361 acgcgctgcc gcacgacctg tccggccgtc gtgtggtggt ctccgcaggc ggcacccggg
     6421 aagccctcga cccggtgcgc ttcctgggca accggtcgtc gggccggcag ggctacgccc
     6481 tcgccagggt cgcggcccag cgtggagcgt cggtcaccct cgtggccgcc cacaccgcag
     6541 acctgggtga cccggcgggg gtggaagtcg ttcgcgtcgg ctccgcggcg cagctacgcg
     6601 acgcgatgta cgcggcggcc aggggcgcgc acgcgctggt gatggccgcc gcggtcgccg
     6661 acttccggcc cgccgcgctc gccgagcaca agatcaagaa aaccagcggc gaccccgagc
     6721 cgctggtgct gaccacgaac ccggacatcc tcgcggagct cgtcacgacc aaggagccgg
     6781 gacaggtcgt ggtcggtttc gccgccgaga ccggcgacgc cgcgggcgac gtcctgcacc
     6841 acggccgcgc caagctcctc cgcaagggct gtgacctgct cgtcgtcaac gcggtgggcg
     6901 atggcaaggc gttcgaggtg ccggacaact ccggctggtt gctcggcgcc gacggcagcg
     6961 agaccgagct gaagcccggc tcgaaggcgc tgctggccac gcgtgtctgg gacgcggtcg
     7021 cttcgcgact ttgagcgcga cgggccgcgg ggcgggcgct gtcagcgggt ccggggaaac
     7081 ccggcgcggc ccgccccgcg ggagccggcg gtcagccgaa cgggccaccg gcgggccgcg
     7141 cccccggtgg ctgcggcgcc ggtgtcggag ccacgccttc cggtgcgcgg agaactggtc
     7201 ggaccggggc ggcgccgtct gccgccacgt actgcgtcga cgcccagccg ccgagatcga
     7261 tcagctggac gaacccctga cgcttctcgc ccacccgcac tccggagccg gcctcgcact
     7321 gcgcgacgat cggcgcgtgg gcgtccggcc cggagcggac attgatgttc tcggtgcagc
     7381 ggccgggttc ggacgctgcg gccgtgccgc cgaccagggc catcaggccg gtcgcgccga
     7441 ccgcgaccat cgcaccgacg acagcggccg cgcggccggg tcggtaggag agcgaggtgg
     7501 aggccttcat cgtcggcttt cctccgtagg gcaggggtct caccgcccca acgagcgccc
     7561 gggtgtccgg tacccgtccc acggacgaga tcaccggaaa ggctgacgga gcgacgggtg
     7621 gagggtccgg ggtgacgccg cgtcacatcc gcccggcgca gtccacctcg gagctcccgc
     7681 tcgggggtcg actaggctgg cccgccggag atcagctgag ctgcggtcgc gacagcggcg
     7741 atcgagcgag aacggacgga gtacggcgtg gcgaacgcag gccggcggtt gttcacgagc
     7801 gagtcggtga ccgagggtca tccggacaag atgtgtgacg ccatcagcga cacggtgctc
     7861 gatgcgctgc tcgctcagga cgcgcgcagc cgcgtggcgg tggagacgct ggtgaccacc
     7921 gggcaggtgc acattgcggg cgaggtcacc acgagcgcgt acgccgacat ccccacgctg
     7981 gttcgggaga agatcctcga catcggttac gactcgtcgg ccaagggctt cgacggcgcc
     8041 tcctgcgggg tcaacgtggc gatcggcgcg cagtcggccg acatcgccca gggcgtcgac
     8101 accgcgtacg agagccgcgt cgagctgagc gaagacgaga tcgcccgcca gggcgccggc
     8161 gaccagggcc tgatgttcgg ttacgccaac accgacacgc cggagctgct gccgctgccg
     8221 atcgcgctcg cgcaccggct ggcgcgccgg ctcaccgacg ttcgcaagtc gggagtgctg
     8281 ccctacctgc gcccggacgg caagacccag gtcaccatcg agtacgacgg cgacaaggcc
     8341 gtccgcctgg acaccgtggt ggtctcgagc cagcacgccg ccgacatcga cctcgacggg
     8401 atgctcgccc ccgacatccg ggagcacgtc gtcgacccgg agatctcgaa gctcggtctc
     8461 gacgtggcgg acctgcggct actggtcaac ccgaccgggc ggttcgtcgt cggggggccc
     8521 atgggcgacg cgggcctgac cggacgcaag atcatcgtcg acacctacgg cgggttcgcc
     8581 cgacacggtg gtggcgcctt ctccggcaag gacccgtcga aggtggaccg gtccggcgcc
     8641 tacgccatgc ggtgggtggc caagaacgcg gtggctgcgg gcctggccga gcgcatcgag
     8701 gtgcaggtgg cctacgcgat cggcaaggct gccccggtcg gcctgttcgt cgagaccttc
     8761 ggcaccgagc acgtcgaccc gctgaagatc cagtcggcga tcaccgaggt gttcgacctg
     8821 cgcccggccg cgatcatccg ggacctcgac ctgctgcggc ccatctacgc ccagaccgcg
     8881 gcatacggac acttcggccg gaccgatgtc gagctgccgt gggagtgcac ctcgcgggcc
     8941 gacgcgctga aggcggcggc gggcggctga ggcccggggc aggcggggcg ggaagcctcg
     9001 aaggcagagg cgcgctgccc tgtcgggcac tcggccggac cgtcgggggt tcggggcacg
     9061 atgccggttg tgagcacgcg gagcaggccg tcacggagcg cctggaccac acggtgacca
     9121 gcagtgccgt ctcgggttcg ccgtcgcggg cgcgcctcag ggtgtccggc ggccgggccg
     9181 gccggcagca aggaggtgcc gtggtgcgac cgggccgcaa ccgcggtgag tggcaggctg
     9241 ctgccgagct gccggtggcc cgagtcgcgg tggatgtagc gctcgcccac ctggaccggc
     9301 cgttcgacta ccgcgtgccc gagcacctcg acgcggtcgc cgtgccggga gtgcggctgc
     9361 gggtgcgctt cgccggccgg ctggtcgacg gcttcctgct ggagcggctg gccgagtccg
     9421 ctcaccccgg cacgcttgcc tgggtggaga aggtggtctc gcccgagccc gtcctcaccg
     9481 ctgaggtggc tgcgctgtgc cgagcggtgg ccgaccggta cgcgggcgtg ctcgccgatg
     9541 tgctgcgcct cgcggtcccg ccccggcacg ccaaggcgga ggccgaggtg ccgccgcagc
     9601 gggccggccc ggtggcggca cccggcgagc caggggcgtg gcgccgttat ccccgcgggc
     9661 ccgccctgct cgatgcgctc gccggcggcc gctctgcgca cgcggtgtgg caggcgctgc
     9721 cgggggagtg ctggccggag cggctcgcgg aggccgccgc ggccaccgtg gccgccgggc
     9781 gtggcgcgtt gctggtggtg cccgaccagc gggacgtcgc cgctctccac ggtgcgtgcg
     9841 ccgggctgct cggcgccgac gctgtcgtgg cgctcaccgc cgagctgggg ccggcggagc
     9901 ggtaccgccg atggctcgcc gtgcgccggg ggcacgtcca ggtcgtcgtg ggcacccggt
     9961 cggcggcgtt cgcgcccgtc gagcgccttg gcctactcgc cgtctgggac gacggcgacg
    10021 acctgcacgc cgaaccgcgg gcgccgtacc cacgggtccg cgacgtcctc gtgctgcggg
    10081 cccacgctgc cggggcggcg ctgctggtgg gcggctacgc ccgcacggca gaggcgcacc
    10141 tgctcgtcga gtccggttgg gccagggagg tcatcgccga gcgtgccacg gtgcgcgcgg
    10201 tcatgccgcg ggtcaccgcg ctcggcgaga ccgacggcca gctcgcccgc gaccccgact
    10261 cccgtgctgc ccggctcccg cacctcgcct tcgaggcggc ccgctccgcg ctgaccgccg
    10321 ggcggccggt gctggtgcag gtcccgcgat cggggtacct gccgtggctc gcctgcggca
    10381 cctgccgcga gaccgctcgc tgccgacact gcgccggtcc actcggcctg accggccacg
    10441 gcacgcgcgg ccgttcgggc ggcgcggtgt tcgggcccac gcctcacgca tccggcacgc
    10501 cgccgcacgc cggctcggcc gggctgccgc attgccgctg gtgcgggcgg gccgacagcg
    10561 cgttccgctg cccggcgtgc gggtcgcgtc ggctgcgggc cggggtcgtc gggtcggggc
    10621 gcacggcgga ggagctcggg cgcgcgttcc ccggcaccac ggtccggtcc tccggcgggg
    10681 gcgcgccagt gctcgccgag gtggcggccc ggccggagct ggtcgtcgcc acccccggcg
    10741 ccgagccgcg cgcggcgggt ggctatggcg ccgcactgct gctcgacgga tgggcgctgc
    10801 tgtcccggcc cgacctgcgc gtggccgagg agacgctgcg gcgctggctc ggcgcagcag
    10861 ccctcgtcgt cccgcacacc gacggtggcc gcgtcgtcgt gatggccgac gccgcgatcc
    10921 ccgtggtgca gtccctggtg cgctgggatc cggccggaca cgccgcagcg gagttcgcgt
    10981 cacgcgccga ggtcggtttc ccgcccgcgg tacggatggc tgctgtcgag ggcacggcgg
    11041 ccgccgtcgc cgacgtgctg gcggagctgc cggaggtcgt cgaggtgctc gggcccgtcg
    11101 agctcgaccc ggtcgggaca gggtccggct cgttcgccaa cacggcggac gagccgccgg
    11161 agatccggga acgcgcgctg ctccgggtgc cccgcgccga cgggcggacc atggccgcgg
    11221 ccctgcatgc cgcgcaggcg gtgcgcaccg cacgcaagga gtccgatccg gtccgggtgc
    11281 gactcgaccc cgtcgagatc ggttgacctg gacgggagga gtggtgcgcg gtccggcgcg
    11341 cgggtacccg cgtggcagaa ttgacggttg tcgcgcatac cggtgcacac gcccaccgcc
    11401 gcccgcttcc gcccgccatt tgctgctgcg aggagatctg tgtccgtcca gcccatcaga
    11461 ttcttcggtg accctgtgct gcgcacgccc gccgtcgagg tgaccacctt cgacgcggag
    11521 ctgcgcaagc tggtctccga tctcacggaa accatgcacg acgagggcgg agccggcctc
    11581 gctgcgcccc agctcggtgt ggggctgcgc gttttcacct acgactgcga agggttcgcc
    11641 gggcatctgg tcaatccgac cttcgaggtg gtgggcgacg aggagcagct ggggccggag
    11701 ggctgcctat cgatcccggg gctgagctgg gactgccggc gacacctgca tgtggtcgcc
    11761 cacggctgga accagcacgg cgagcccgtc acggtcgagg gcagcgagaa gctggcccgc
    11821 tgcatccagc acgagatcga ccatctcgac ggtgtgctgt tcgtcgaccg cctcgactcg
    11881 gagacccgca aacaggccat ggcggagatc cgcgccgccg agtggttcgg cggatccgag
    11941 ccgcgggtgc aggtcagccc gcacccgctc ttcggaaagg ccaggtagcg gcccgtgaag
    12001 ctggtgttcg ccggcacgcc ggagcccgcg gtgccgtccc tgcgggcgct gctcggctcg
    12061 cctcgccacg aggtggccgc cgtggtgacc cggcctgacg ccccagccgg ccggggtcgc
    12121 aagatcgtcc ggtcgccggt gggcacgctc gcggacgagg ccggtatccc ggtgctgacc
    12181 ccggcccggc cgtctgaccc ggcgttcctg gcgcagctcg ccgagctcgc gccggactgc
    12241 tgcccggtgg tggcctacgg cgcgctcgtg ccgcgcgccg cgttggacat cccgcgccat
    12301 ggctgggtga acctgcactt ctccctgctg ccggcgtggc gcggcgcggc gcccgtgcag
    12361 gctgcggtgc gccacggcga cgaggtcaca ggcgctacca cgttcctgct cgaggcgggc
    12421 ctcgacaccg ggccgacctt cggcgtcgtc accgaggccg tcggcccgca ggacaccgcg
    12481 ggcgacctgc tcgacaggct cgcggtgtcc ggtgcggggt tgctgctggc gacgatggac
    12541 ggcatcgccg acggcacgct gcggccccgg gcgcagccgt ccgacggggt ctcgcacgcc
    12601 cccaaggtga ccacggccga cgccagggtc gattgggcag cgccgcccct ggcagtggac
    12661 cggctgatcc ggtcggtcac ccccgaaccc ggcgcctgga ccaccttccg cgccgaccgg
    12721 ctcgggctcg gcccggtcgg cgcgacgacg gacgcccccg ggggggacgc cgggctaccg
    12781 gtgctgaagc cgggcgaact gcacgtggag aagcgccggg tgctggtcgg caccgccgcc
    12841 gccccgctgg agctgggcga ggtgcagccc gtgggcaagc gagcgatgcc ggcgcccgac
    12901 tgggcacgcg gagtgcgtat cgagccggga gagctgctga catgaggcac cgaccacagc
    12961 gcgcggccag gttggcgagc acggcggctc cggcagtgcg gaggatcgtg tgagcacgcc
    13021 gcgccggcgg ccacgccggg gggcgggggc gcccgggcgg gccgatgcgg gaggggccga
    13081 cgctgcgcgc cgggccgggc cgccgcgtgg gcgcagcggg ccgccgcgcc caccggagct
    13141 ggatccgtcg cggcaggctg cactcgacct gctcaccgcg gtgcgggtgc gtgacgccta
    13201 cgccaacctc gccctcccgt cgatcctgcg gcgataccgg ctgcacgacc gggacgccgc
    13261 gttggccacg gagctcggct acggcacgct gcgggcccgc ggcctgctcg acgccgtgat
    13321 cgaggcatgc acggaccggc cgctcatccg cgtcgagccc gcgctgctcg acgcgctccg
    13381 actcggggcc taccagctgc tacgcacccg gatcccggcg cacgccgctg tggacacgac
    13441 tgtggagctc gtgcgcggcg atgccggcag ccgggccgcg gggttcgtca acgcgatcct
    13501 gcgcaaggtc ggggaacgtg acgagcaggc gtgggtggag cgcctggcgc cgcccgcgga
    13561 tgaagacccg gtgggccacg cggcgatggc gcacgcccac ccccgctgga tcgcccagtc
    13621 gttcgccgac gcgctcggcg ccccggagga gctcgacgct gcgctcgcgg ccgacgacgc
    13681 gcgccccacc gtgcatctgc tggcgcggcc gggggagatc accgccgcgg agctggcgct
    13741 cgtgaccggc ggcgaggagg cgccgtactc cccgtacggc gtgcacctcg aacccggcgg
    13801 tggcgacatc gcggagatcg acgcggtggc agaagggctt gccgtcgtgc aggacgaggg
    13861 cagccagctg atggcgctcg ccctgtcgcg ggccgcgctc gccggcgccg acaccggccg
    13921 atggctggac ctgtgcgccg gtccgggcgg gaaggcggcg ctgctgggcg gcatcgccgc
    13981 actggacggt gcatctgtgg atgccgtaga ggtgagcgag caccgcgccg atctggtgcg
    14041 ccgtgccacc gacgggctcc cggtgaccgt ccacaaagcc gacggccgcg ccgcgccgct
    14101 acccgagggg acctacgacc gcgtgctggt ggacgcgccg tgcaccgggc tcggcgcgct
    14161 gcggcggcgg ccggaggcgc gttggcggcg gcggccggag gatgtgtcgg ggctgacgaa
    14221 gctccagcgc gagctgctca ccgcagccct gcggcacgtc cggcccggcg gagtggtggc
    14281 gtacgtcacc tgctctccgc acctcgcgga gacggtgggt gtgcttaccg gggtactgcg
    14341 caggcatccg gacgtggaac agctcgacgc cagggaatac ctccccgaca tgcccgacct
    14401 cggtgccggc ccgacggtgc agctgtggcc acatcgtcac gggacggatg cgatgttcct
    14461 cgcgctgctg cgccggaccg catgaccggg ctcaccggcc tctggtgccc gaggcccggt
    14521 cgagcgccca gtcgacggcc aggtcgaggt actcgacaac cagctcctcc tcgtcgggac
    14581 ctgcgtccac ctcgatgcgg acatcgcgca tacgacggcc ctcgccgagc agcctgccgt
    14641 gtggatccgg caggtcggcc ccgtgctcga aaccgacatt gacgccgccg tcgcgcggga
    14701 acagtgcgca cacgtatcca ccacgcgggt ggtggaaacc gagcccgtgc caccccgggt
    14761 agacccgctc gcgcaggtcg ggatgggcga tgcggaccac cgcgcggagc cggcggacga
    14821 gtacggcgac ggcaggcagc tcgccgacga gcaggtgatc gagctgctcg tcgatggaca
    14881 caggtgcggg catgggtgaa gtggaccgca gggcaccgac agttctggga gccgacggcg
    14941 gctggttctg ctcggcgtaa ccttccgcgg gtgcacccga tgatcgcccc gagcattctc
    15001 gcggccgact tcgcgcggct cgccgacgag gccgccgccg tggcagggga gcccggtcat
    15061 ggcgccgact ggctccacgt cgacgtgatg gacgcgcact tcgtgcccaa cctcacgatc
    15121 ggcctgcccg tcgtggaatc gctgatccgg gccacgacga tccccctgga ctgccacctg
    15181 atgatcgacg acccggaccg ctgggccgtg ggctacgccg aggcgggcgc ccacaacgtg
    15241 acggtgcatg tcgaggccgc tcgcgacccg gtgggactgg ccgcggatct gcgggcagcc
    15301 ggtgcgcgcg cgggcctctc ggtcaagccg ggcacgccgc tggagccgta cgtggagacg
    15361 ttgcggcact acgacaccct tctcgtgatg agtgtggagc ccgggttcgg cgggcagagc
    15421 ttcatgcccg aggtgctcga caaggtgcgt gcggcgcggc ggatggtgga caccgggcac
    15481 ctgacgctgc tcgtggagat cgacggcgga atcaacgcag agacgatcga ggcggccgcc
    15541 gaggccgggg tcgactgctt cgtggccgga tcggccgtgt acagcgccga cgatccggcg
    15601 cgcgcggtgc aggccctgcg ggagcaggtg ctcggggcct cggcgcaccg tcggtggaac
    15661 cagtgatcac ccgccccggg cgtcgaggat gatcgcgacg ggccgggtaa cgattcgcgg
    15721 cggttcacgg tttcagaccg tgtcggtcgg gcgttggaat gggagctcgt agatgttcac
    15781 gggaatcgtc gaggagatcg gtgaggtgct cgccgtgcgc gagagcggcg aggtcgtggt
    15841 gctgaccgtg cgcgggcgga ccgtcaccgc cgacgcgggc cacggtgact cgatcgccgt
    15901 gaacggcgtg tgcctcaccg tgatcgaccc ggccggcagc agcgacggca ccttcacagt
    15961 cgagctcgtg cccgagacgc tgaaacgttc gagtctttcc gccgtggccg agggggccag
    16021 ggtgaacctg gagcgcgcgg tgccggtgac cggtcggctg ggtggccaca tcgtgcaggg
    16081 ccatgtcgat ggggtggcca cgctgctctc ccgcacaccg ggggagcgca gcgacgagct
    16141 gcggttcagt ctcgccccgg accttgcccg gtacgtagtg gagaagggct ccatcgctgt
    16201 ggacggcgtg tcgctgacag tggccgccgt cacacctgac ggcttcacgg tggcgctgat
    16261 ccccacaacg ttgtcgcaca ccacactcgg tctgcgccag gttggggata ccgtcaacct
    16321 ggaagtcgac gtcgtcgcga agtatgtgga gcggctcgcc gccgggtaca cgggccgtac
    16381 cggtatcacg gcgacggtcg gcgaggaggc cagagcgcag t
//
LOCUS       DASRSC010000103         8355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1016105,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000103 DASRSC010000000
VERSION     DASRSC010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016105"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VFE39_04970"
     CDS             <1..140
                     /locus_tag="VFE39_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6257986.1"
                     /translation="AYKEIAGELFISVKTVETHVSSVLRKTQLSNRYELSRWASDRRL
                     V"
     gene            complement(163..1164)
                     /locus_tag="VFE39_04975"
     CDS             complement(163..1164)
                     /locus_tag="VFE39_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257987.1"
                     /translation="MTPVSVVTVADLLSRNAPAPAKLEADDVPTTGISVGSLLRREGR
                     APHALDRPMQPRAHQPAAQQPTEQADEHAAEPTSKRVLVRRSAVAAGALLAVGSVLGA
                     AALTHVAPTGNEAQGNSYPGQGLLDPSGTAPVLPGTAVIDPAAATGALDPGTAPPASW
                     VPVAFPSAIAGIPAAVGAPATGTVKSPASNSTGAGVSGTNGTGSGASGSGGGTPSTNS
                     GGGSTATNTQGGSSGGVVQDVGSSVGGTVENVGGALPAPLDQSVQDVGGVVSDTASTV
                     DQLTAPLTSLLSNQSSNDNSSNDNSSSNDNSGSDNGGGNSSGLLSGVGNVAAGLLGG"
     gene            complement(1334..2092)
                     /locus_tag="VFE39_04980"
     CDS             complement(1334..2092)
                     /locus_tag="VFE39_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET6257988.1"
                     /translation="MATPLADDVRPRATVRTDVERALVVTAHPDDVDFGAGGTVAAWT
                     AAGIEVAYCICTSGDAGGYDDTPRAAMGPLREAEQLAAAAAVGVQDVQFLRYPDGRLT
                     ASIELRRDISREIRRFRPDRVLTQSPEIWWRRLGASHPDHRAAGEAALAAVYPDARNP
                     FAHPELLAEEGLDAWTVGELWVMGAPDERVDHVVDVTETFDHKIAALRAHHSQTGHWP
                     DLDGRIRTWNADWAARSGLPGGRLAEAFQVVPIG"
     gene            complement(2145..2465)
                     /locus_tag="VFE39_04985"
     CDS             complement(2145..2465)
                     /locus_tag="VFE39_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6257989.1"
                     /translation="MGTVFGIVAAVIALVGLLAALGHVGYLAMLNNAAKKRGLSGGTT
                     TDYVNSRWKVAGGTAGVALLALLMTTSDSVPVDVLGLLLGGGAGLYAKQALDSTRTRF
                     RGLP"
     gene            complement(2519..2830)
                     /locus_tag="VFE39_04990"
     CDS             complement(2519..2830)
                     /locus_tag="VFE39_04990"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET6257990.1"
                     /translation="MTVTTGNNTAQVGDGPVDDVPAEDEIQELRVEIDHLDAEILRLV
                     KRRSEVSQRIGRARMAAGGPRIVYNREMAVLARFRELGSEGRELGMLLLRLGRGRLGG
                     R"
     gene            3032..5368
                     /gene="pcrA"
                     /locus_tag="VFE39_04995"
     CDS             3032..5368
                     /gene="pcrA"
                     /locus_tag="VFE39_04995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET6257991.1"
                     /translation="MSALFELPTENPVHARTERGVALLEGLNPRQREAVVHAGSPLLI
                     VAGAGSGKTRVLTHRIAWMLAERGVHPGEIMSITFTNKAAAEMKERVDALVGRRANAM
                     WVSTFHSMCVRILRREAQHLGVRSAFSVYDADDTRRLVGLVARDLELDPKKFAARGLA
                     AQISNLKNELLSPDDAADRATNEYERKVAEVYAVYQARLRTANSFDFDDLIMETVALL
                     QRLPAVAEYYRRRFRHVLVDEYQDTNHAQYALVRELVTPAAEGAQPGELCVVGDSDQS
                     IYAFRGANIRNIIEFEKDFPDARTILLEQNYRSTQRILSAANAVIARNPDRRDKRLWS
                     EQGDGEKVVGYVADNEHDEAAFVAQEIDRLVDSGVARNSDVAVFYRTNSQSRVFEDVF
                     LRVGLPYKVVGGVRFYERREVRDALAYLRVLSNPEDTVSLRRILNVPKRGIGDRAEEL
                     VATYADRERISFAAALRTAAEQPERVPGLATRSQRCIAAFVRILDDLAELVEKGEQTA
                     ELLEAIYAKTGYTAELEASEDPQDGSRLENLAELVTVAREFAGDAAVADLDDSDVAVG
                     APAPGSLAAFLERVALVADADSIPDNDEGMVTLMTLHTAKGLEFPVVFLTGWEDGVFP
                     HMRAMGDPAELAEERRLAYVGITRAQQRLYLTRALIRSSFGQPNANPASRFLAEVPGD
                     LVDWRRSEPERSAPVGRFGFGRRPAATDRGSWNVPQRSLNATISLDVGDRVNHDKYGL
                     GTVIASDGAGARATVTIDFGSAGTVRLMLIGGVPLQKL"
     gene            complement(5401..6255)
                     /locus_tag="VFE39_05000"
     CDS             complement(5401..6255)
                     /locus_tag="VFE39_05000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET6257992.1"
                     /translation="MARHRSPSGAQADDPDRTAPLRVAAVPSGHRLPAPPAASLRGRA
                     TVAAVAAGAVVAAGQTLASAFGADALAPQTVSALVPVADVTEAADTNVAVDAIGGDQL
                     LPEPLALNALDPGAQVDVQNLAKAVDLGRELTRQAAIIDAALSKGAPEAHLYRDLAFV
                     RPAVGRLTSVFGTRWGVAHNGIDIAGPIGTPIYALTDGVVEEAGPATGFGLWVVLRHT
                     DGTQSAYGHVNRMFVDVGEKVKAGEEIAEIGNRGYSTGPHLHLEIWDSDGTKLNPIPW
                     LRKRGIDF"
     gene            complement(6454..7413)
                     /locus_tag="VFE39_05005"
     CDS             complement(6454..7413)
                     /locus_tag="VFE39_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6257993.1"
                     /translation="MALVRAGLGRLAEVAAAAQTAATEVGRVGAYIARTFGAPAGLRG
                     LAVEWAWLAAHLVIYPAGLLREQLAEPTGYRTDSLPPVRRSLLVTDVEAAGTPILLVH
                     GIMDNRSVFTVFRRSLRRRGFGAVHAVNYSFFTGDIRAAAYQLRGHVEELRERTGADR
                     VHIVGHSLGGMIARYYVQRMGGTAAVDTLVTLGSPHSGTLTAHLMPTPLARQLRPGSD
                     LLEELSRRAPGCSTRFLVVWSRMDQMVVPQRNARLTHPDLDVEEFELRDVGHLSLPID
                     PRTVHWVATALTRSDQTRHPFRRGQQPVDEMEDTDGQTASPAS"
     gene            7619..>8355
                     /locus_tag="VFE39_05010"
     CDS             7619..>8355
                     /locus_tag="VFE39_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET6257994.1"
                     /translation="MDLYEYQAKDLFAAHGVPVLPGRTVETPEEAAVAAAELGTAVVV
                     KAQVKTGGRGKAGGVKLATTPDEAKEKAAAILGLDIKGHTVHRVLVTEASDIAEEYYF
                     SFLLDRSNRTFLAMASAEGGMEIEQLAVERPEALAKVPIDAIAGVDKAKAADIVAQAK
                     IPAAVADEAADVIVKLWETFVSEDATLVEVNPLVRDPQDRVIALDGKVTLDENAGFRH
                     PDHAGLVDERTENPLEAKAKSKGLNYV"
BASE COUNT         1232 a         2843 c         3040 g         1240 t
ORIGIN      
        1 acgcgtacaa ggaaatcgcc ggggagctgt tcatctcggt gaagacggtg gagacgcacg
       61 tgtcgagcgt gctgcgcaag acgcagctgt ccaaccgcta cgagctgtcg cggtgggcgt
      121 cggaccggcg gctcgtgtag ctaccggtcc gaccttccgg ggtcagccgc ccagcaggcc
      181 agcggcaaca ttgccgacgc cgctgaggag cccggaggaa ttcccgccgc cgttgtcgct
      241 gccgctgttg tcgttgctgc tgctgttgtc gttgctgctg ttgtcgttgc tggactggtt
      301 gctcagcaga gaggtgagcg gggcggtgag ctggtcgact gtggaggccg tgtcgctgac
      361 gacgccgccg acgtcctgaa ccgactggtc gaggggagcc gggagtgccc cgccgacgtt
      421 ctcgacggtg ccaccgacgc tcgacccgac gtcctggacc accccaccgg acgatccacc
      481 ctgggtgttg gtcgcggtgc ttccgccgcc actgttcgtg gacggtgtgc caccgcccga
      541 tccgctagca ccgctgccgg tgccgttcgt gccggagaca cccgcaccgg tgctgttcga
      601 ggcagggctc ttcacggtgc ctgtggccgg ggcgcccacc gcggcgggga tgcccgcgat
      661 cgcgctgggg aacgcaaccg gcacccacga cgccgggggt gccgtgcccg ggtcgagcgc
      721 cccggtggcg gcagcggggt cgatcacggc ggttccgggg aggacgggtg ccgtgccact
      781 cgggtcgagc agaccctgtc ccgggtagct gttgccctga gcctcgttgc cggtgggcgc
      841 gacatgagtg agcgctgcag cgccgagcac tgatcccacg gcgaggagcg cgcctgccgc
      901 gacagcgctg cgacgcacca gcacgcgctt cgaggtcggc tcggcagcgt gctcgtcggc
      961 ctgctcggtg ggctgctggg cggcgggctg gtgcgcccgc ggctgcatgg ggcggtcgag
     1021 cgcgtgcgga gcacggccct cgcggcgcag caggctgccg acggatatgc cggtggtggg
     1081 gacgtcgtcc gcctcgagct tcgcaggcgc cggggcgtta cgactcagca gatcggcgac
     1141 ggtgaccaca ctgacaggag tcattcgtgg gccacctcgg gacaccatct gagccactgt
     1201 gcaacgtcct ccctttgagt gaacggacga gcagatgtct accggatgcg taactcccat
     1261 accaagtgtc attcgtccgc tcgtcatctt tcaccctaat ggagtattcc attcgtcgac
     1321 tgttatctac cgctcatccg atcggcacga cctggaacgc ctcggcgagc ctcccgcccg
     1381 gtagcccgga gcgtgcggcc cagtccgcgt tccaggtgcg aatacggccg tccaggtcgg
     1441 gccagtgtcc cgtctgcgag tggtgcgcgc gcagcgcggc gatcttgtgg tcgaacgtct
     1501 cggtcacgtc cacgacgtgg tcgacgcgct cgtccggcgc ccccatcacc cacagctcgc
     1561 cgaccgtcca cgcgtcgagt ccctcctcgg ccagcagctc cgggtgggcg aaggggttgc
     1621 gcgcgtcggg gtagacagcg gcgagtgccg cctcgcccgc cgcgcggtgg tcggggtgcg
     1681 acgcgcccag gcgacgccac cagatctccg gtgactgtgt cagtacccgg tcgggacgga
     1741 acctacggat ctcgcggctg atgtcgcggc gcagctcgat gctcgctgtc agccggccgt
     1801 cggggtaccg gaggaactgg acgtcctgta ctccgaccgc agccgccgcg gccagctgct
     1861 cggcctcccg cagcggaccc atcgcggccc gcggcgtgtc gtcgtagccg ccggcgtcgc
     1921 cggatgtgca gatgcagtag gcgacctcga tgcccgccgc cgtccacgcg gcgaccgtgc
     1981 cgcctgcgcc gaagtcgacg tcatcaggat gggcggtgac gacgagcgcg cgttcgacgt
     2041 ccgtccggac ggtggcgcgg gggcgaacgt catcagcgag gggcgtggcc accggcgaac
     2101 tctacgagcg tggccgaccg ggcaggccga tcagggcagg ccgatcaggg caggccgcga
     2161 aagcgcgtgc gggtggagtc gagtgcctgc ttcgcgtaga ggccggcgcc gccgccgagc
     2221 agcaggccga gcacgtcgac cggtaccgag tccgacgtcg tcatcagcag ggcgagcagc
     2281 gcgacgcctg cagtgccgcc cgcgaccttc cagcggctgt tgacgtagtc ggtggtggtg
     2341 ccgccggaca ggccgcgctt cttggccgcg ttgttcagca tggccaggta accgacgtgt
     2401 cccagcgcgg cgaggagccc caccagagcg ataaccgctg cgacgatccc gaacacggtc
     2461 cccatgacgt caacagtgcc acgaaacgac gtcgaggggg tggacccacc gggtggactc
     2521 agcggccgcc gagcctgccg cgtcccagcc ggagcagcaa cattccgagc tcgcggcctt
     2581 cggagccgag ctcgcggaac cgcgcgagca ccgccatctc ccggttgtag acgatgcgcg
     2641 ggccgcctgc ggccatccgc gccctgccga tccgctgcga gacctcgctg cggcgtttca
     2701 ccaggcgcag gatctcggcg tcgagatggt cgatctcgac ccgcagctcc tggatctcgt
     2761 cctcggcggg gacgtcgtca acggggccgt cgcccacctg tgcggtgttg ttgcctgtcg
     2821 tcacggtcat gctctcctcc aggtctggtg tcctggcccg agacgcgaga acgccccggg
     2881 tcctgaggac tccggggcgt cgtgggggct gatcagctgg ccacgggcac cggtgtccgg
     2941 tacccgtaga aaaatcgatg gctggtcagt cgcacgtccg cagtgtgcca caccggggcc
     3001 cgtcggggcg cggcggtact cttcgaacac gatgagtgcg ctgttcgaac tgccgaccga
     3061 gaatcccgtc catgcccgca ccgaacgcgg cgtggcgctg ctggaggggc tcaacccgcg
     3121 ccagcgcgag gcggtggtcc acgccggttc gcccctgctg atcgtcgcgg gagcggggtc
     3181 gggcaagacg cgcgtgctca cccaccgaat cgcctggatg ctggccgagc gcggggtgca
     3241 ccccggcgag atcatgtcga tcaccttcac caacaaggcc gccgcggaga tgaaggagcg
     3301 cgtcgacgcg ctggtgggcc gccgcgccaa cgcgatgtgg gtctccacgt tccactcgat
     3361 gtgcgtgcgg atcctgcgcc gcgaggcgca gcacctcggc gtgcggagcg ccttctccgt
     3421 ctatgacgcc gacgacacgc gcaggctcgt cgggttggtg gcccgcgacc tggagctgga
     3481 ccccaagaag ttcgccgccc gggggctcgc cgcccagatc tccaacctca agaacgagct
     3541 gctctccccg gacgacgccg ccgaccgcgc caccaacgag tacgagcgca aggtcgccga
     3601 ggtctacgcc gtctaccagg cccggctgcg cacggccaac tcgttcgact tcgacgatct
     3661 gatcatggag acggtcgcgc tgctgcagcg gttgcccgcg gtcgcggagt actaccggcg
     3721 gcgcttccgg cacgtgctcg tcgacgagta ccaggacacc aaccacgcgc agtacgcgct
     3781 ggtgcgcgag ctggtcactc ctgcggccga gggtgcgcaa ccgggggagc tgtgcgtggt
     3841 gggcgactcc gaccagtcga tctacgcgtt ccgcggcgcc aacatccgca acatcatcga
     3901 gttcgagaag gacttccccg acgcccggac gatcctgctc gagcagaact accgctccac
     3961 ccagcggatc ctgtccgcgg ccaacgccgt gatcgcgcgc aaccccgacc gccgtgacaa
     4021 gcggttgtgg tcggagcagg gcgacgggga gaaggtcgtc ggctacgtgg ccgacaacga
     4081 gcacgacgag gccgcgttcg tcgcccagga gatcgaccgg ctggtcgatt ccggcgtggc
     4141 ccgcaactcc gacgtggccg tgttctaccg caccaactcc cagtcccggg tgttcgagga
     4201 cgtgttcctg cgggtcgggc tgccgtacaa ggtcgtcggt ggcgtccggt tctacgagcg
     4261 gcgcgaggtc cgcgacgcgc tggcctacct gcgtgtgctg tccaacccgg aggacacggt
     4321 cagcctccgg cgcatcctca atgtccccaa gcgggggatc ggggaccgcg ccgaggagct
     4381 ggtggccacc tacgccgacc gggagcgcat ctcgttcgcc gccgccttgc gcaccgctgc
     4441 cgagcagcca gaacgtgtcc ccggtctggc cacccgctcg cagcgctgca tcgcggcgtt
     4501 cgtccgcatc ctcgacgacc tcgccgagct ggtggagaag ggtgagcaga ccgccgagct
     4561 gctggaggcg atctacgcga agaccggtta cacggccgag ctggaggcca gcgaggaccc
     4621 ccaggacggg tcacggctgg agaacctggc cgagctcgtc acggtcgcgc gggagttcgc
     4681 gggcgacgct gccgtggccg acctcgacga ctccgatgtg gcggtgggcg cacccgcacc
     4741 gggttcgctg gccgcgttcc tcgagcgcgt cgcgctggtg gccgacgccg actcgatccc
     4801 ggacaacgac gagggcatgg tcacgctgat gaccttgcac accgccaagg gcctggagtt
     4861 cccggtcgtg ttcctcaccg ggtgggagga cggggtgttc ccgcacatgc gcgccatggg
     4921 cgaccccgcc gagctcgccg aggagaggcg gctcgcctac gtcggcatca cgcgtgcgca
     4981 gcagcggctg tacctgaccc gggcgctgat ccgttcctcg ttcgggcagc ccaacgccaa
     5041 cccggcttcg cggttcctcg cggaggttcc cggcgacctg gtcgactggc ggcggtcgga
     5101 gccggaacgc tcagcgccgg tgggccggtt cggattcggg cggcgccccg ccgccaccga
     5161 ccgcggcagc tggaacgtgc ctcagcgttc gctcaacgcc acgatcagcc tcgacgtcgg
     5221 cgaccgcgtc aaccacgaca agtacggtct cggcacggtg atcgcctccg acggtgccgg
     5281 ggcgcgcgcc accgtcacga tcgacttcgg gagtgcgggc acggtacggc tgatgctcat
     5341 cggtggggtt ccgctgcaga agctgtgagc cggggtcgac gggcgtcggg aggccccgcg
     5401 tcagaagtcg atgccgcgtt tcctgagcca ggggatcggg ttgagcttcg taccatcgct
     5461 gtcccagatc tccaggtgca ggtgcggccc ggtggagtag ccacggttgc cgatctcggc
     5521 gatctcctcg cccgccttca ccttctcgcc tacgtcgacg aacatgcggt tcacgtgccc
     5581 gtacgcgctc tgggtgccgt cggtgtggcg gagcaccacc cagaggccga agccggtggc
     5641 cggacccgcc tcctccacga cgccgtcggt gagcgcgtag atcggggtgc cgatgggtcc
     5701 ggcgatgtcg atgccgttgt gggccacgcc ccagcgcgtg ccgaacaccg acgtgaggcg
     5761 accgaccgcc gggcgcacga acgccaggtc gcggtacagg tgcgcctcgg gagccccttt
     5821 ggacagcgcg gcgtcgatga tcgccgcctg ccgggtcagc tcacggccga ggtcgacggc
     5881 cttggccagg ttctgcacgt cgacctgggc gccggggtcc aacgcgttca gtgcgagcgg
     5941 ttcgggcagc agctggtcgc caccgatcgc gtcgacggcg acgttggtat cggcggcctc
     6001 ggtcacgtcg gcgacaggga cgagcgcgct cacggtctgc ggggcgaggg catcggcgcc
     6061 gaacgcagag gcgagtgtct ggccagccgc aaccaccgcg cctgccgcga ccgcggccac
     6121 tgtggcccgg ccgcgcagcg acgcagcggg tggcgccggg agacggtgcc cgctcgggac
     6181 cgcggcgacg cgcaggggcg ccgtgcgatc ggggtcgtcc gcctgggcgc cgcttgggga
     6241 gcggtggcgc gccaagtcct gcccttctcg acgtctaacc ggtggtgttc gcgtcccgct
     6301 cgcgacagcg cgggctgggg atcccacagc ggcgatcggg gccaccgctg ctgctgtcgt
     6361 gaccggcctg tgacacggac ggtgcgaacg tagcggcggt cgcacgtagc gggcaaatct
     6421 ggcacggcgt gtcggacgcg gatccgacga cgatcaggac gccggggacg ctgtctgacc
     6481 gtccgtgtcc tccatctcgt ccactggctg ctgacctctg cggaacgggt gacgagtctg
     6541 gtccgaacgg gtgagggcgg tggctaccca gtgcaccgtg cgtggatcga tcgggagcga
     6601 caagtgaccg acatcacgta gctcgaactc ctcgacgtcg agatcagggt gcgtcagccg
     6661 agcgttgcgt tgcggaacga ccatctggtc catccggctc cagacgacca ggaaccgggt
     6721 ggaacacccg ggcgcccgcc tgctgagctc ctcgagcaga tcggagcccg gtctcagctg
     6781 gcgggccagc ggggtcggca tcagatgggc cgtgagggtg ccgctgtgcg ggctgccgag
     6841 ggtgaccagc gtatcgacgg cggccgtgcc gcccatgcgc tgcacgtagt accgcgcgat
     6901 catcccgccg agcgagtggc cgacgatgtg caccctgtcg gcgccggtgc gctcgcgcag
     6961 ctcctccacg tggccgcgga gctggtatgc ggcggcgcgg atgtcgccgg tgaagaagct
     7021 gtagttcacg gcgtgcacgg cgccgaaccc gcggcggcgc agcgagcggc ggaacacggt
     7081 gaacaccgac cggttgtcca tgatcccgtg gaccagcagg atcggcgtcc cggctgcctc
     7141 cacgtcggtg acgagcaggc tgcgccgcac cgggggcagg gagtccgtcc ggtacccggt
     7201 gggttcggcg agctgctcgc ggaggagccc ggccgggtag atcaccaggt gcgccgcgag
     7261 ccatgcccac tcgacggcga gaccgcggag gccggccggg gcgccgaacg tgcgcgcgat
     7321 gtaggcaccc actcgcccga cctcggtggc agccgtctgc gccgccgcgg ccacctcggc
     7381 caggcggccc agccccgcgc ggaccagcgc catgtgtcga cggtaagtga caaacggccg
     7441 tagcgcccgt tccgagtttt cgctaagggg cgtcgagcac gtcctgtcgg gggcgtgaca
     7501 gcggtcacca gggcaggtca gagcgggtac ggcgggcgtc gctagggtcg cgtcggcagc
     7561 agattcgcgg gccggaggcc cgcagcggcg cagcgcagtg ggcatctagg gagagcaagt
     7621 ggacctgtac gagtaccagg cgaaagacct cttcgccgcg cacggtgtcc cggtgttgcc
     7681 gggccggacc gtggagacgc cggaggaggc ggccgtggcg gctgccgagc tcggcacggc
     7741 ggtcgtggta aaggcgcagg tgaagacggg cggccggggc aaggcgggtg gcgtcaagct
     7801 cgcgacgacg ccggacgagg ccaaggagaa ggccgcggcg atcctcggcc tggacatcaa
     7861 gggccacacg gtgcaccgcg tgctggtcac cgaggccagc gacatcgcgg aggagtacta
     7921 cttctcgttc ctgctcgacc ggtccaaccg caccttcctg gccatggcgt cggccgaggg
     7981 cggcatggag atcgagcagc tcgccgtgga gcggccggag gcgctcgcga aggtgccgat
     8041 cgacgcgatc gccggcgtgg acaaggcgaa ggccgccgac atcgtggccc aggcgaagat
     8101 cccggccgca gtggccgacg aggccgccga cgtgatcgtg aagctgtggg agacattcgt
     8161 ctccgaggac gcgaccctcg ttgaggtgaa cccgctggtc cgtgatccac aggacagggt
     8221 catcgcgttg gacgggaagg tgacgctcga cgagaacgcc ggtttccggc atcccgacca
     8281 tgctgggctg gtcgatgagc gtacggagaa cccgttggag gcgaaggcga agtcgaaggg
     8341 tctcaactac gtcaa
//
LOCUS       DASRSC010000104         8161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_524465,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000104 DASRSC010000000
VERSION     DASRSC010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8161
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524465"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..950)
                     /locus_tag="VFE39_05015"
                     /pseudo
     CDS             complement(18..950)
                     /locus_tag="VFE39_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237192.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            990..1538
                     /locus_tag="VFE39_05020"
     CDS             990..1538
                     /locus_tag="VFE39_05020"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6257995.1"
                     /translation="MAGLSMGGQIALELHRRHPDRVAGLLLADTTATGERSRADRLAM
                     ADRLLREGMDPYAMEMLYRMVTRGAPSAVADHVLAMMRATDPTGAAAAQRARADRPDH
                     RTDLRSVVVPTTVVVGSEDELTPVSDARVIAELVPDAELVVVDRAAHLPNLEQPAIFD
                     RALSRLLGRVDRPGDRSAIKTG"
     gene            1676..2803
                     /locus_tag="VFE39_05025"
     CDS             1676..2803
                     /locus_tag="VFE39_05025"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET6257996.1"
                     /translation="MRVQRWVLLVVAVVVWAMPSLGGDTAHVAEADAVARAGDDREKD
                     GSDSAQRKPSRDSGGEEAAPAETPPAEQKPSAPAPSKKPATAGPALAGDLLAVVRGRG
                     AAIEVADTSELERALVAARPGDTIRMAAGSYGPVVITRSGTAAAPITLTGPSDAVITG
                     SGKGYTLHLDGGSHWQLVGFAVSGGGKGIMTDGSTGLLLDSLTVSDTGDEAVHFRGGS
                     SDNTIQRSTIRDTGLNQPEYGEGVYVGSAKSNWKKYSNGRPDLSMNNRVVGNTFKRIT
                     AENVDVKEETRGTLIAGNRFDGSAISGENFADSVVDVKGYGGTVTGNLTTGNAPALKN
                     IIETHVITEPETSGCGNRIEDNRVEGFRPAGALVAVDKKCR"
     gene            complement(2825..3172)
                     /locus_tag="VFE39_05030"
     CDS             complement(2825..3172)
                     /locus_tag="VFE39_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6257997.1"
                     /translation="MKQYLLSIYQPEGDPPPPEVLDPVMRKLDALNEEIRAAGAWVFA
                     AGLHPPSTATVLRSHGDEVLITDGPFAEGKEHLGGFTIIRAPDLDAALAWGRRLAEVI
                     TLPIEVRPLYGED"
     gene            complement(3530..4534)
                     /locus_tag="VFE39_05035"
     CDS             complement(3530..4534)
                     /locus_tag="VFE39_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HET6257998.1"
                     /translation="MIVITPADAASPDHLPEAERGNGQWTDGSPWTRVEDQVDREKSG
                     ARWMTPQEREQAVTDAVLASFEQTKDDRLRELLESLTRHLHGFASEVRLTQDEWETAI
                     GFLTRVGHITDDRRQEFILLSDVLGLSMLTVGINAPVSSAATESTVFGPFFVEGAPEV
                     PLGGDIAGGAKGTPCWVSGTVSSTDGTPIAGGRIEAWEADEDGLYDVQYEGNRTAGRG
                     WLRSGPSGEYRFWSVRPSAYCIPDDGPVGELLAAAGRGAMRPAHLHLKVDAPGHRTLI
                     THLFAAGDPNLDRDAVFGVKDSLIVEFVEHRPGTAPDGRILDSSWASVTFDIVLEPAA
                     "
     gene            4524..5501
                     /locus_tag="VFE39_05040"
     CDS             4524..5501
                     /locus_tag="VFE39_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET6257999.1"
                     /translation="MTIIDRARITSLHVFCAVLVAAILVRPLITEALSAPVLRTAATV
                     FVAVCVQALPFLVLGVLLSGAIAAFVSPALLRRALPAHPMAAVPVAGAAGLVLPGCEC
                     ASVPVARRLMAQGVPDGAALTFLLAAPAINPVVLVATAVAFPGEPRMVLARAIGSLAT
                     ACVMGWLWQRAGRAGWIAARARRAAPASSGAGRWAVLAETARHDLVSAGGFLVLGGLT
                     AAAFSVLVPPEWMDALAGQVLLAVVVMAVLAVVLALCSEADAFVAASLSMLPLLPRLA
                     FLVVGPAVDVKLIALQAGTFGQAFAARFAPATFVVAVMCAVAAGLVVLP"
     gene            5498..6208
                     /locus_tag="VFE39_05045"
     CDS             5498..6208
                     /locus_tag="VFE39_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET6258000.1"
                     /translation="MRRDTQHVLLVLLGGALLRIAADDTYLRYVRPSHRWLLLAAGAV
                     IVVLAAVALVRDRAPAAVSPGEGTHQHTGVERHVPWLLLAPVLVIVLVAPPALGADAV
                     SRSGSRNAALQDADLFGPLPPGDAPELSVAEFVQRAAWDTSGSVEGREVTLIGFAVRR
                     GAAMELARMTISCCAADARPNRVRLVGDLGEITPDTWLRVQGSAHPGSATAASGYVPA
                     LTVTMLEQVDPPPDPYEY"
     gene            complement(6233..6931)
                     /locus_tag="VFE39_05050"
     CDS             complement(6233..6931)
                     /locus_tag="VFE39_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258001.1"
                     /translation="MNRVLDVARVQAVNWRGIVAWPLGILAFTLVINVAIFGAIGDAA
                     PPEGRVTGALSSIYIVMFVAHLQTITQVFPFALGLSVTRRAFFAGTALVVVAQALFFG
                     LVLLLLEWVERTTGGWGVGVRYFGLPFLIQENPLTQWLVYVVPFLAVSAIGVFMGVVF
                     KRWGQAGMYVLTMTAGVLAAGFAIVVTWQRSWPSVGSFFTGQSTFALLAGYPLVIALV
                     LGGVGWFTIRRATP"
     gene            complement(6928..7833)
                     /locus_tag="VFE39_05055"
     CDS             complement(6928..7833)
                     /locus_tag="VFE39_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6258002.1"
                     /translation="MNVVRMSGVTKRYGQVTALDGVSMELAENRIHGLLGRNGAGKTT
                     VMQILTGQAFETSGGVEVFGEHPYENAGVLGRICFIKESQRYPDFFKVKHALRAAELL
                     YPHWDAGFAAELVADFALPADRNIRKLSRGMLSAVGVIIGLASRAPLTFFDEPYLGLD
                     AVARQLFYDRLLADYAEHPRTVVLSTHLIDEVSDLIEHVVLIDKGRILIDEDAEALRT
                     RAVTVTGPVASVESFVAGRTVLHREQLGGFLRVTVSGVPDRPSVGGLEFEAVSLQQLV
                     VRTTQMNGLHNGQPVAAGTGNGGNR"
     gene            complement(7830..8159)
                     /locus_tag="VFE39_05060"
     CDS             complement(7830..8159)
                     /locus_tag="VFE39_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6258003.1"
                     /translation="MAEQIESSIVDGSLAEETQVPSTNELAAFHRINPATAAKGVSQL
                     VADGLLYKRRGIGMFVATGARTQLLERRREEFARQYLAPLLVEARKLGIDVEHIKKMI
                     DSWGDEG"
BASE COUNT         1168 a         2971 c         2873 g         1149 t
ORIGIN      
        1 tgatcactcc cgtccgctca ctccaccccg tcgtcccccg gtgctcccct ggccaggccg
       61 gcgaggacgt cgagcgcggc ggccagcacc ggcaccggtg gcgcagcgag tgcgaggcgc
      121 acggcgttcg gcgcgtgggc cgcccccacc gcgaatgccg cggccggagt gaccgcgatg
      181 ccacgccggg cggccgcggc cacgaacgtc tccgcccgcc acggcgccgg aagctcccac
      241 cagcagtggt aggcggccgg gtcggcgcgc accgcgaacc cggcgagccg ttcggcgacg
      301 agcgcctggc gcgccgcggc gtcggcacgc ttggccgact cgatctccgt gaccacccca
      361 ccggccatcc agcgggtggt cgcctccagc gcgaacgcct gggccgtcca cccaccggag
      421 cgcagcgcag cggcgacccg gtcctgtacc gccgcgggcg gaacgacgaa accgagcgtc
      481 aacccgggcg cgacgcgctt ggacaggctg tcgacgagga cggtccgctc cggcgcgagc
      541 gccgccagcg gagcgggacc gtcccgcagg aacgcgtaga tcgtgtcctc cacggcgggc
      601 acgtcgcgct cggcgagcac gtcggcgagc gccgcccggc ggcccggcga catcgtcgtg
      661 ccgagtgggt tgtgcagcgt cggctgcagg tagacggcgt cgagccggcc cgcggcccgc
      721 acggcgccgg gcaccaggcc gtcggcgtcc atggcgaggg ggacgagttc cacacccagc
      781 cgggcggcga ccgcccgcac caccggatag gtcagggcct cgacgcccag cctgccaccg
      841 accggtacca gcgcaccgac tgccgcggcg atcgcctgcc gcccgttgcc ggcgaacagc
      901 acccgctccg gtgcaggtgc ccacccctgc cgggccagca cggtggtggc gcgggcctgc
      961 tcgacggcct gcgcgtacca cgtgccgtcg tggccgggct gtcgatgggc gggcagatcg
     1021 cactggagct gcaccggcgt caccccgacc gcgtggccgg actgctgctg gccgacacca
     1081 cggcgacggg tgagcgttcc cgtgcggacc gactggccat ggccgaccgg ctgctccgcg
     1141 agggcatgga cccctacgcc atggagatgc tctaccggat ggtcacacgg ggagccccga
     1201 gcgcagtcgc cgaccacgtg ctcgccatga tgcgggccac cgaccccacc ggtgccgccg
     1261 cggcccagcg cgcccgcgcc gaccgcccgg accaccgaac cgatctccgg tccgtcgtcg
     1321 tgcccacgac cgtggtcgtc gggtcggagg acgagctcac cccggtctcc gacgcgcgcg
     1381 tgatcgccga gctcgtgccg gacgccgagc tggtggtcgt cgaccgcgcg gcccacctgc
     1441 ccaacctcga gcaaccggcg attttcgacc gcgcgctgtc ccgcctgctc ggccgcgtcg
     1501 accggcccgg tgaccggtcg gcgatcaaga ccggctgatc atcggcgaga tgatcgaaac
     1561 cgccgcgggg catccatccc gccccctagc ctcacacctg ctcgatgcca ggtgctgatc
     1621 gctgcgccga caggcagcga cagggacagc gatccgcgag tgcggaggtc atgagatgcg
     1681 ggttcagcgg tgggtgctgc tcgtcgtggc ggtcgtcgtg tgggcgatgc cgtcgctcgg
     1741 cggtgacacc gcccacgtcg ccgaggcgga tgcggtcgcc cgcgccgggg atgaccgcga
     1801 gaaggacggt tccgactccg cgcagcgaaa gccctcccgc gactccggcg gggaggaggc
     1861 cgcaccggcg gaaacgccac cggcggagca gaagccctcg gcgcctgctc cgtcgaagaa
     1921 gcccgcgacg gccggtccag cactcgccgg cgatctgctg gctgtggtcc ggggccgcgg
     1981 cgccgccatc gaggtcgccg acacctccga gctcgaacgg gcactggtcg ccgcgcgacc
     2041 gggcgacacg atccggatgg ccgccggcag ctatggcccc gtcgtcatca cccgctccgg
     2101 caccgccgcc gcacccatca cgctgaccgg cccgtccgac gcggtgatca cggggagcgg
     2161 caagggctac acgctgcacc tcgacggggg cagccactgg cagctcgtcg gcttcgcggt
     2221 ctccggtggc ggcaagggga tcatgaccga cggcagcacc gggctcctgc tcgactcgct
     2281 caccgtgagc gacaccggcg acgaggcggt gcacttccgc ggcggatcca gcgacaacac
     2341 gatccaacgc tcgacgatcc gcgacaccgg gctgaaccag cccgagtacg gcgagggcgt
     2401 ctacgtcggc agcgcgaaga gcaactggaa gaagtacagc aacggccgcc cggacctgtc
     2461 gatgaacaac cgggtggtgg gcaacacgtt caagcggatc accgcggaga acgtggacgt
     2521 caaggaggag acccgcggca ccctgatcgc cggcaaccgc ttcgacggtt ctgccatcag
     2581 cggcgagaac ttcgccgact cggtggtgga cgtgaagggc tacggcggca ccgtcaccgg
     2641 caacctcacc accggcaacg cccccgccct gaagaacatc atcgagacgc acgtgatcac
     2701 cgagccggag acgtcgggct gcggcaaccg catcgaggac aacagggtgg agggcttccg
     2761 gcccgcgggc gcgctggtgg cggtcgacaa gaagtgccgg tgacgccggt caagccggga
     2821 acggtcagtc ctcgccgtac agcggccgca cctcgatcgg aagcgtgatc acctcggcga
     2881 gcctgcggcc ccacgcgagc gcggcgtcga ggtcgggcgc cctgatgatc gtgaagccgc
     2941 ccaggtgctc cttgccctcg gcgaacgggc cgtcggtgat cagcacctcg tcgccgtggc
     3001 tgcgcagcac ggtggccgtg ctcggcgggt gcagacccgc ggcgaagacc cacgcgcccg
     3061 cggccctgat ctcctcgttc agcgcatcga gcttccgcat gaccggatcc aggacctcgg
     3121 gcgggggtgg gtcgccctcg ggctggtaga tgctcagcag gtactgcttc atggcagaca
     3181 gcgcctcctc aggtatcagc ggtcggcagg ccggtaggtg gcgatcagaa caccggtggt
     3241 cgtcggaacg cactcgacga gccggagtct ggcgaatgcg gcgctgggtc aggttctcga
     3301 ccacggtgag ggtgcccacg gcattcctcc tcggcatcgg ctgccggcct cgtaccggcg
     3361 tcccaccctc tgtacgaacg gggcccggcc ggttcgacag cggaaacgag gaatctccgt
     3421 ggatgtgtcg gacggccttc gcctgcccat cacaggcccc ggcctgatca gccgcgggcg
     3481 agcacggtgg aactaccgat caccagcacg tccgtctgca gatcccttgt cacgccgctg
     3541 gctccaacac gatgtcgaag gtgacactgg cccaggacga gtcgaggatc cgaccgtccg
     3601 gggcggtacc cggacggtgc tcgacgaact ccacgatcag gctgtccttc acgccgaaca
     3661 cggcgtcccg gtccaggttc gggtcacctg cggcgaacag gtgggtgatc agggtgcggt
     3721 gcccgggggc atcgaccttc agatgcaggt gcgccgggcg catcgcgccg cgtcctgccg
     3781 cggccagcag ctcgccgacc gggccgtcgt cggggatgca gtaggcggac ggacgcacgg
     3841 accagaagcg gtactccccc gacggccccg acctcaacca cccgcgcccg gcggtgcggt
     3901 tcccctcgta ctgcacgtcg tagaggccgt cctcgtcggc ctcccacgcc tcgatgcggc
     3961 cgccggcgat cggagtgccg tccgtcgagc tgacggtgcc cgacacccag cacggcgtgc
     4021 ccttcgcccc gcctgcgatg tccccgccga gcggcacctc cggggcgccc tccacgaaga
     4081 acgggccgaa caccgtggac tcggtcgccg ccgaggacac cggggcgttg atcccgacgg
     4141 tgagcatcga gagcccgagg acgtcggaga gcaggatgaa ctcctggcgg cggtcgtcgg
     4201 tgatgtgccc gacgcgggtg aggaacccga tcgccgtctc ccactcgtcc tgggtgagcc
     4261 ggacctcgct ggcgaacccg tgcaggtgcc gggtgaggct ctccaggagc tccctcagcc
     4321 ggtcgtcctt cgtctgctcg aagctggcca agaccgcgtc ggtcaccgcc tgctcgcgct
     4381 cctgtggcgt catccacctg gccccgctct tctcgcgatc aacctgatcc tccaccctcg
     4441 tccacgggct gccgtccgtc cactgcccgt tgccgcgttc tgcctccgga aggtggtcgg
     4501 gactggcagc atcggccggc gtgatgacga tcatcgaccg cgcccggatc acctcgctgc
     4561 acgtgttctg cgcggtgctg gtagccgcga tcctcgtccg cccgctgatc accgaggcac
     4621 tgtcggctcc ggtgctgcgc accgcggcca cggtgttcgt cgccgtgtgc gtacaggcgc
     4681 tgccgttcct ggtgctcggg gtgctgctgt cgggcgcgat cgccgcgttc gtctcccccg
     4741 cgctgctccg ccgcgccctg cctgcgcacc ccatggcagc cgtgcccgtc gccggtgccg
     4801 ccgggctggt gctgcccggc tgcgagtgcg cgtccgtgcc ggtggcgcgc aggctcatgg
     4861 cgcagggtgt gccggacggc gccgcgctca cgttcctgct cgcggcaccg gcgatcaatc
     4921 ccgtcgtgct ggtcgccacg gcggtggcct ttccgggcga gccccggatg gtgctcgccc
     4981 gcgccatcgg atcgctggcc accgcctgcg tgatgggctg gttgtggcag cgcgccggac
     5041 gcgcaggctg gatcgcggcg cgggccaggc gggccgcgcc ggcctcgtcc ggtgcgggac
     5101 ggtgggcggt gctcgccgag accgcccggc acgacctcgt ctcggcgggc ggcttcctgg
     5161 tgctcggcgg gctcacggcc gcggcgttct cggtgctggt accgcccgag tggatggacg
     5221 cgctggcggg gcaggtgctg ctcgcggtcg tcgtcatggc ggtgctggcc gtggtcctcg
     5281 cgctgtgcag cgaggccgac gcgttcgtgg cggcgtcgct gtcgatgttg ccgctgctgc
     5341 cccgcctggc gttcctcgtc gtcgggcctg cggtggacgt caagctgatc gcactgcagg
     5401 cgggcacatt cgggcaggcg ttcgccgccc ggttcgcgcc ggcgacgttc gtggtggccg
     5461 tgatgtgcgc ggtcgcggcc gggctggtgg tgctgccgtg aggcgcgaca cccagcacgt
     5521 cctgctcgtg ctgctcggcg gcgcattgct gcgtatcgcc gccgacgaca cctacctgcg
     5581 ctacgtgcgc ccgtcgcacc gttggttgct gctcgcggcg ggcgcggtga tcgtggtgct
     5641 cgctgccgtg gcgcttgtgc gcgaccgcgc ccccgccgcg gtgagccccg gggaaggcac
     5701 tcaccagcac accggagtcg agcggcacgt cccctggctg ctgctcgcac cggtgctggt
     5761 gatcgtgctg gtggcaccac ccgcgctcgg cgcggacgcc gtctcccggt cggggtcacg
     5821 caatgcggcg ctgcaggacg ccgacctgtt cgggccgctc ccgccgggtg acgcgcctga
     5881 gctttccgtc gccgagttcg tgcagcgggc ggcgtgggac accagcggct cggtcgaggg
     5941 ccgcgaggtc acactgatcg ggttcgccgt ccgtcgcggt gccgccatgg agctcgcccg
     6001 catgaccatc tcctgctgcg ccgccgacgc ccgcccgaac cgggtgcggt tggtcggcga
     6061 cctcggcgag atcacgcccg acacctggct gcgcgtgcag ggcagcgcgc acccgggcag
     6121 cgcgacggcg gccagcgggt acgtcccggc cctgacggtc acgatgctgg agcaggtgga
     6181 tccgccaccc gatccgtacg agtactgatc ggcacccgca caacggcagc ggctacggcg
     6241 tggcgcgccg gatcgtgaac cagccgaccc cgccgagcac gagcgcgatc accagcgggt
     6301 agcccgccaa cagtgcgaac gtcgactggc cggtgaagaa cgagccgacc gagggccacg
     6361 agcgctgcca ggtgacgacg atcgcgaacc cggccgccag cacgcctgcg gtcatggtca
     6421 gcacgtacat gccggcctgg ccccatcgct tgaagaccac gcccatgaac accccgatcg
     6481 ccgacaccgc gaggaacggc acaacgtaga ccagccactg ggtgagcggg ttctcctgga
     6541 tcaggaacgg cagcccgaag tacctgacgc cgacacccca gccaccggtc gtccgctcca
     6601 cccattcgag caggagcagg acgagcccga agaacagtgc ctgcgccacg accacgagcg
     6661 cggttcccgc gaagaaggca cgccgggtga cgctcaaccc gagggcgaac gggaacacct
     6721 gcgtgatcgt ctgcaggtgc gccacgaaca tcacgatgta gatcgacgac agtgctccgg
     6781 tgactcggcc ctccggcgga gcggcgtccc cgatggcgcc gaagatcgcc acgttgataa
     6841 cgagagtgaa agcgaggatc cccagcggcc aggcgacgat cccccgccag ttgaccgcct
     6901 ggaccctggc gacgtcgagc acgcggttca tcggttcccc ccgtttccgg tgccggcggc
     6961 gaccggctga ccgttgtgca gaccgttcat ctgggtggtg cggacgacga gctgctgcag
     7021 agacaccgct tcgaactcca ggccgccgac ggacgggcgg tcggggacgc cggagacggt
     7081 gacgcgcagg aagccaccga gctgctcacg atgcagcacg gtgcgcccgg cgacgaagga
     7141 ctcgacggag gcgacggggc cggtgacggt gaccgcacgg gtgcgcagcg cctcggcgtc
     7201 ctcgtcgatg agaatccgcc ccttgtcgat caggacgacg tgctcgatga ggtcactgac
     7261 ctcgtcgatg aggtgggtgg acagcacgac ggtgcgcggg tgctcggcgt agtcggcgag
     7321 cagccggtcg tagaacagct ggcgcgccac cgcgtcgagg ccgaggtagg gctcgtcgaa
     7381 gaacgtcagg ggtgcccgcg aggcgagccc gatgatcacg ccgacggcgg atagcatgcc
     7441 gcgggagagt ttcctgatgt tccggtcggc cggcagcgcg aagtcggcca ccagctcggc
     7501 ggcgaacccg gcgtcccagt gcgggtagag cagctcggcg gcccgcagag cgtgcttgac
     7561 cttgaagaag tcgggatatc gctggctctc cttgatgaag cagatccggc ccagcactcc
     7621 cgcgttttcg tacgggtgct ccccgaacac ctccacccct ccggaggtct cgaaggcctg
     7681 gccggtgagg atctgcatca ccgtggtctt gccggcgccg ttgcgcccga gcaggccgtg
     7741 gatccggttc tcggcaagct ccatgctcac cccgtcgagg gcggtgacct gcccgtaccg
     7801 cttcgtcacc ccgctcatcc tgacgacgtt catccctcgt ccccccagga atcgatcatc
     7861 ttcttgatgt gctcgacgtc gatgccgagc ttgcgggcct ccaccagcag cggcgccagg
     7921 tactgccgcg cgaactcctc ccgccggcgc tccagcaact gcgtccgagc cccggtcgcg
     7981 acgaacatcc cgatccctcg tctcttgtag agcaacccgt cggccacgag ctggctgacg
     8041 cccttcgccg cggtggccgg gttgatgcga tggaaggccg ccagctcgtt ggtcgagggc
     8101 acctgcgtct cctcggccag cgatccgtcg acgatcgagc tctcgatctg ctcggcgatc
     8161 t
//
LOCUS       DASRSC010000105         7965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_198566,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000105 DASRSC010000000
VERSION     DASRSC010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198566"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..378)
                     /locus_tag="VFE39_05065"
     CDS             complement(151..378)
                     /locus_tag="VFE39_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HET6258004.1"
                     /translation="MSTTTVPPHVRLANDVAAQFAHRPLDVAAAAVATHVRDFWEPRM
                     TRALLAHVAAGGDALDPVAVRAAQLLGPAAG"
     gene            complement(382..3207)
                     /gene="fdhF"
                     /locus_tag="VFE39_05070"
     CDS             complement(382..3207)
                     /gene="fdhF"
                     /locus_tag="VFE39_05070"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HET6258005.1"
                     /translation="MTLLKEPDFGTPAREGAATVELTVDGVPVAVPPGTSVMRAAKHA
                     GVDVPKLCATDSLEAFGSCRLCVVEIDGARGTPASCTTPVAPGMVVRTQTERLGKLRR
                     GVMELYISDHPLDCLTCSANGDCELQDMAGVTGLREVRYGSGVEAGANHMDAPTDTSN
                     PYFDFDASKCIACSRCVRACDEVQGTLALTIEGRGFDSKVAAGAGVSFMESDCVSCGA
                     CVQACPTATLQEKSVVQLGMPTRSVVTTCAYCGVGCSFKAELRGDELVRMVPYKDGGA
                     NEGHSCVKGRFAFGYASHPDRVLSPMLRESIADPWREVSWEEAIGFTARRLREIQAEH
                     GPGSIGAITSSRTTNEEVYVVQKMVRAVFGNNNVDTCARVCHSPTGYGLKQTFGTSAG
                     TQDFKSVAKADVIMVIGANPTDGHPVFASRMKRRLRQGAKLIVIDPRRIDLVRSPHIE
                     ATHHLQLAPGTNVAVVNAMAHVIVTEGLVDRAFVQERCEEVAAWEAFIAGPEHSPEAV
                     EEITGVPAAEVRAAARLYAEAGNGAIYYGLGVTEHSQGSTMVMGMANLAMATGNIGRD
                     GVGINPLRGQNNVQGACDMGSFPHELPGYRHVSDDGARDVYEQLWGTPLLDEPGLRIP
                     NMFDSAIDGSFRALFVQGEDIAQSDPNTQHVHAALAALDLVVVQDLFQNETAKFAHVL
                     LPGTSFLEKDGTFTNAERRINRVRPVMAPKTGKQEWQIICEIAQAMGYPMHYDSSAQI
                     MDEIALTTPTFAGVSFAHLDRVGSVQWPCNEASPSGTPIMHQDVFVRGKGRFVETVYV
                     PTRERSTRKFPLILTTGRILSQYNVGAQTRRTANVAWHPEDILELHPHDGEVRGINDG
                     DMVTLSSRVGRTTLRAVLAERMPVGVCYTTFHHPVTGANVITTDNSDWATNCPEYKVT
                     AVQVALSPEQAAVREDLAQQPVATAGD"
     gene            complement(3204..4796)
                     /locus_tag="VFE39_05075"
     CDS             complement(3204..4796)
                     /locus_tag="VFE39_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HET6258006.1"
                     /translation="MDAPVRVFVPRDAAAVSVGADEVAERIVAAAVTTGRAVEVVRTG
                     SRGMLWLEPLVEVETPAGRVGYGPIAPEDVDGLVAAGLLDGTPGGLGLVEDLPWMRRQ
                     QRLTFARVGIVDPRSADDFEAHGGNAGLRAALAMEPSAVVEEVVGSGLRGRGGAGFPT
                     GIKWRTVLGATADIKFVCCNADEGDSGTFADRMLIEGDPFTLIEGMTIAAHAVGATEG
                     YVYLRSEYPDAVETLTAAIETARARGWLGERILGSDLTFDLHVRVGAGAYICGEETSM
                     LESLEGKRGTVRAKPPIPALEGLFGRPTVVNNVLSLATVPTILANGAVAYAAYGMGRS
                     LGTNVFQLGGNIARGGIVETAFGITLGELVEDLGGGAASGRPVRAVQVGGPLGAYLPA
                     AQFDLPMDYESFAAAGAMVGHGGIVVFDDTVDMSAMARFAFSFCAEESCGKCTPCRVG
                     AVRGVETIDRIRAGEDRAGNLVLLDDLCELMTDGSLCAMGGLTPLPVRSAVQHFGEDF
                     GIPVTSTAGTTSTAATTTEGTS"
     gene            complement(4829..5311)
                     /locus_tag="VFE39_05080"
     CDS             complement(4829..5311)
                     /locus_tag="VFE39_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HET6258007.1"
                     /translation="MTTDVTDLAAVRAEHVRHIATTLRDVRGPLLPILHAVQEDLGYV
                     DPADVPVIADVLNLSVAEVHGVVTFYRDFRRAPAGRTTVRICQAEACRSVGAEALGEH
                     AKRRTGVGFGETTSDGAITLDEVFCLGNCALAPAVQVDERLHGRVSPARFDALLGEAR
                     "
     gene            complement(5380..6315)
                     /locus_tag="VFE39_05085"
     CDS             complement(5380..6315)
                     /locus_tag="VFE39_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6258008.1"
                     /translation="MLLRQLEYLSALARERHFARAAAACHVSQPALSAAIRRLEADLG
                     VQIVRRERRFGGFTPEGERVVVWAHRMLADQDALRQDLDRMAGGLTGKLRIGAIPTAL
                     TAAPLLTAPFSERHARVRISFESMSSREIVRKLADYELDVGMTYVDGEPLGGAVRTVP
                     LYRERYLLLTPDDSPLADRTTAAWDDAATLPLCLLPPVMENRRILDRAFAAAGVAVEP
                     MLEADAVSVLYAHVATHRWSTIISHAWLHLFGVPDGMRIVPMTTPRQSHRIGLVLPDR
                     LPEPMLVRAVLDVAGGLDLRAELDDLLERHVHGSS"
     gene            6513..7679
                     /locus_tag="VFE39_05090"
     CDS             6513..7679
                     /locus_tag="VFE39_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HET6258009.1"
                     /translation="MTSRTVPAAPSRWAQLRTTLTREDRAAIGGMAGFVVLLHVVGFG
                     VLIAVVVPAHYDLGSTGTYGIGVGVLAYTLGMRHAFDADHIAAIDNTTRKLIADGGRR
                     PLSVGFWFSLGHSTVVFGLTFLLAFGVKALVGEVENGGSRLHSIGGTIGATVSGTFLW
                     ILGILNLVVLIGIIRIFRRMRAGEYDEQELEEQLNKRGFMNRFLSGLTRSIAKPWHIY
                     PVGVLFGLGFDTATEVGLLVLAGGAAAFSLPFYAILVLPILFAAGMSLLDSLDGVFMN
                     FAYSWAFARPVRKVFYNITITALSIAVALVIGTIELVSVITQQAGIDSGPLAAVASIP
                     LNDAGYGIVALFALAWLVAFAIWRLGHVEQRWSATPGEPGAATGTGGEAPETVA"
     gene            complement(7695..>7965)
                     /locus_tag="VFE39_05095"
     CDS             complement(7695..>7965)
                     /locus_tag="VFE39_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6258010.1"
                     /translation="AAAKAALHGLVHNLSANVAGSGVTINAIAPALIAGTRMLPLGPD
                     EPVPARIPAGRLGTTEEVADLSLAMLRNGYLTNKVIGLDGGAYPD"
BASE COUNT         1145 a         2739 c         2869 g         1212 t
ORIGIN      
        1 tcgacggagc caccacggga gcggccagca cggcgccgtc cgcgtcggcg atcgggaccg
       61 accgcacggg cagcgggagc gcggccgcgt gcgcgcgctc ccgggcgccg tcccaggtga
      121 gggacgtcgt cggcggagcg aacgcggtgc tcatccggct gcggggccca gcagctgggc
      181 cgctcgcacg gccaccgggt cgagcgcgtc gccgcccgct gcgacgtgcg ccaagagcgc
      241 cctcgtcatc cgcggctccc agaagtcgcg cacatgcgtg gccacggcgg cggccgcgac
      301 gtcgagcggt cggtgcgcga actgcgccgc gacgtcgttg gccagccgga cgtgcggggg
      361 aacagtggtg gtcgacatcg atcagtcccc tgccgttgcg accggttgct gcgccaggtc
      421 ctcgcgcacg gccgcctgct cgggggacag ggccacctgc acggccgtga ccttgtactc
      481 cgggcagttg gtggcccagt cggagttgtc ggtggtgatc acgttggccc ccgtgactgg
      541 gtggtggaag gtcgtgtagc acacgccaac gggcatccgt tccgccagca ccgctctcag
      601 agtagtccgc ccgacccggc tggagagcgt caccatgtct ccgtcgttga tcccgcgcac
      661 ctccccgtcg tgcgggtgca gctcgaggat gtcttcaggg tgccaggcga cgttggcggt
      721 gcggcgggtc tgcgcgccca cgttgtactg cgacaggatc cggccggtgg tgaggatcag
      781 cgggaacttc cgcgtgctgc gctcgcgcgt gggcacgtag accgtctcga cgaagcggcc
      841 cttgccgcgc acgaacacgt cctggtgcat gatcggcgtg ccgctcggcg acgcctcgtt
      901 gcacggccac tgcacgctgc ccacccggtc gaggtgggcg aacgagacgc cggcgaaggt
      961 gggcgtggtg agggcgatct cgtccatgat ctgcgccgaa gagtcgtagt gcatggggta
     1021 gcccatcgcc tgcgcgattt cgcagatgat ctgccactcc tgcttgccgg tcttcggcgc
     1081 catcaccggg cgcacccggt tgatccgccg ttcggcgttg gtgaacgtgc cgtccttctc
     1141 caggaacgac gtgccgggca gcagcacgtg ggcgaacttc gccgtctcgt tctggaagag
     1201 atcctggacg acgacgagat ccagcgcggc gagcgcggca tgcacgtgct gggtgttggg
     1261 gtcggactgc gcgatgtcct cgccctgcac gaacaaggcc cggaacgacc cgtcgatggc
     1321 tgagtcgaac atgttcggga tgcgcaggcc gggctcgtcg agcagcggag tgccccagag
     1381 ctgctcgtag acgtcgcggg cgccgtcgtc ggagacgtgc cggtagccgg gcagctcatg
     1441 cgggaacgag cccatgtcgc aggcgccctg cacgttgttc tgcccccgca acgggttgat
     1501 gcccaccccg tcgcggccga tgttgcccgt ggccatcgcg aggttcgcca tgcccatgac
     1561 catcgtcgag ccctggctgt gctcggtgac gccgaggccg tagtagatgg cgccgttgcc
     1621 ggcctccgcg tagagccggg ccgcggcccg cacctcggcg gcgggcacgc cggtgatctc
     1681 ctcgactgcc tcggggctgt gctccggacc ggcgatgaac gcctcccacg cggcgacctc
     1741 ctcgcagcgc tcctgcacga acgcccggtc gaccaggcct tccgtgacga tgacgtgcgc
     1801 catcgcgttg accaccgcca cgttcgtgcc gggtgcgagc tgcaggtggt gggtggcctc
     1861 gatgtggggg gagcggacga gatcgatccg ccgcgggtcg atgacgatca gcttggcgcc
     1921 ctggcgcagc cgccgcttca tccgggaggc gaacacaggg tggccgtcgg tcgggttggc
     1981 gccgatcacc atgatcacat cggccttggc gacggacttg aagtcctggg tgccggcgga
     2041 ggtgccgaac gtctgcttga gcccgtagcc ggtgggggag tggcagacgc gggcgcaggt
     2101 gtcgacgttg ttgttgccga acaccgcgcg caccatcttc tggacgacgt agacctcctc
     2161 gttcgtcgtc cgggacgagg tgatcgcgcc gatcgaacct ggcccgtgct cggcctggat
     2221 ctcgcggagc cgccgcgccg tgaacccgat ggcctcctcc caggagacct cgcgccacgg
     2281 gtcggcgatc gactcgcgca gcatggggga gagcacgcgg tcggggtggc tcgcgtagcc
     2341 gaaggcgaac cgtcccttga cgcaggagtg gccctcgttg gcgccgccgt ccttgtaggg
     2401 caccatccgc accaactcgt cgccgcgcag ctcggccttg aacgagcagc cgaccccgca
     2461 gtacgcgcag gtggtgacca ctgacctggt gggcatgccc agctgcacca ccgacttctc
     2521 ctgcagggtg gccgtggggc aggcctgcac gcatgcgccg caggacacgc agtcggactc
     2581 catgaaggag acgccggcgc ccgcagccac cttcgagtcg aagccgcggc cctcgatcgt
     2641 gagggccagg gtgccctgca cctcatcgca ggcgcggacg cagcgcgagc acgcgatgca
     2701 cttcgaggcg tcgaagtcga agtaggggtt ggacgtgtcc gtgggcgcgt ccatgtggtt
     2761 ggcgccggcc tcgacgcccg acccgtagcg gacctcgcgc agccccgtga ccccggccat
     2821 gtcctgcagc tcgcagtcgc cgttggccga gcaggtgagg cagtccagcg ggtggtcgga
     2881 gatgtagagc tccatcaccc cgcggcgcag cttcccgagc cgctcggtct gggtgcgcac
     2941 caccatgcca ggggcgacgg gggtggtgca ggacgccggc gtgccgcggg cgccgtcgat
     3001 ctcgacgacg cacagccggc aggagccgaa cgcctccagg ctgtcggtgg cgcacagttt
     3061 ggggacgtcc accccggcgt gtttcgccgc gcgcatcacc gacgtgcccg ggggcacagc
     3121 gacgggtacg ccgtcgaccg tcagctcgac ggtggccgcg ccctcacggg cgggcgttcc
     3181 gaagtcgggc tccttgagca gggtcatgac gtgccctccg tggtcgtagc cgccgtgctg
     3241 gtcgtgcctg ccgtgctggt gacgggtatg ccgaagtcct caccgaagtg ctggaccgcg
     3301 ctgcgcaccg gtagcggggt gaggccgccc atggcgcaga gcgatccgtc ggtcatcagc
     3361 tcgcacaggt cgtcgagcag gacgaggttg cctgcgcggt cctcgccggc gcggatgcgg
     3421 tcgatcgtct cgacgccgcg caccgcaccc acccgacagg gggtgcactt gccgcaggac
     3481 tcctcggcgc agaacgagaa ggcgaagcgg gccatcgcgg acatgtcgac ggtgtcgtcg
     3541 aagacgacga tcccaccgtg gccgaccatc gccccggccg cggcgaacga ctcgtagtcc
     3601 atcggcaggt cgaactgcgc ggccggcagg tacgccccga gcgggccgcc cacctgcacc
     3661 gcccgcacgg ggcgcccgga cgcggcgccg ccgccgaggt cctcgacgag ctcgcccagg
     3721 gtgatcccga aagccgtctc gacgatcccg ccgcgggcga tgttgccgcc gagctgaaag
     3781 acgttggtgc cgagcgagcg gcccatgccg taggccgcgt aggcgacggc gccattggcc
     3841 aggatcgtcg ggacagtagc cagcgagagc acgttgttga ccacggtcgg gcggccgaag
     3901 aggccttcga gtgcggggat cggcggcttg gcgcgcacgg tgccgcgctt gccctcgagg
     3961 ctctcgagca tcgaggtctc ctcgccgcag atgtaggcgc cggcgcccac ccgaacgtgg
     4021 aggtcgaacg tcaggtccga gccgaggatc cgctcgccga gccagccccg cgcccgggcc
     4081 gtctcgatgg cggcggtcag tgtctcgacg gcgtccgggt actccgagcg caggtagacg
     4141 tacccctcgg tggcgcccac cgcgtgggcc gcgatcgtca tgccttcgat cagggtgaac
     4201 gggtcgccct cgatgagcat ccggtccgcg aatgtgccgg agtcgccctc gtcggcgttg
     4261 cagcagacga acttgatgtc ggccgtggcg ccgaggaccg tacgccactt gatcccggtc
     4321 gggaaaccgg cgccgccccg gccgcgcagc ccggaaccca caacctcctc gaccacggcg
     4381 gacggctcca tcgcgagcgc ggcccgtagc cccgcgttgc ccccgtgggc ctcgaagtcg
     4441 tcggccgagc ggggatcgac gatcccgacc ctggcgaacg tcagccgctg ctggcggcgc
     4501 atccacggca ggtcctcgac gagcccgagt ccgcccgggg tgccgtccag caggcccgcc
     4561 gcgaccaggc cgtcgacgtc ctcgggtgcg atcggcccgt acccgacccg gcccgcgggc
     4621 gtctcgacct cgaccagtgg ctccagccag agcatgccgc gcgacccggt gcggaccacc
     4681 tcgaccgcgc gtcccgtggt gacggcagcc gcgacgatcc gctcggccac ctcgtcggct
     4741 cccaccgaca ccgccgcggc gtcgcgcggg acgaacaccc gcactggtgc atccacgcgc
     4801 cgtgtccgtg attcgctcgc aagctcgctc atcgggcctc tcccagcagt gcgtcgaagc
     4861 gggcggggct gacccggccg tggagccgtt cgtccacctg caccgctgga gcgagcgcgc
     4921 agttgccgag gcagaacacc tcgtcgagcg tgatggcgcc gtcggaggtg gtctcgccga
     4981 agccgacgcc tgtgcggcgc ttcgcgtgct cgccgagcgc ctcggcaccg acggagcggc
     5041 acgcctcggc ctggcagatc cgcacggttg tgcggccggc gggcgcgcgg cggaagtcgc
     5101 ggtagaaggt gacgacgccg tgcacctcgg cgaccgagag gttgagcacg tcggcgatca
     5161 ccggaacatc cgcgggatcg acgtacccca gatcctcctg tactgcgtgg aggatcggca
     5221 ggagcgggcc gcgcacgtcc cgcagcgtgg tcgcgatgtg ccgcacgtgc tcagcacgca
     5281 ctgccgcaag gtccgtgacg tccgtcgtca tgcggcgact gtcccggtct gaggtggttc
     5341 aggtcaaagc aatctctgtt atcccctgat aatcggcgcc tatgagctgc cgtgtacgtg
     5401 cctttcgagt aaatcgtcca gctcggctcg aaggtccaac ccgcccgcga catcgagcac
     5461 ggcccgcacg agcatcggct ccggaaggcg gtcggggagc accagtccga tgcggtggct
     5521 ctgccgcgga gtggtcatcg gcacgatgcg catcccgtcc gggacgccga acaggtgcag
     5581 ccaggcgtgg gagatgatcg tcgaccagcg gtgggtggcg acgtgggcgt agagcacgga
     5641 caccgcgtcg gcctccagca tgggttccac ggcgacgccg gccgccgcga aggcgcggtc
     5701 gaggatccgg cggttctcca tgaccggtgg cagcaggcac agcggcagcg tcgcggcgtc
     5761 gtcccaggct gcggtggtcc ggtccgcgag cggcgagtcg tcgggcgtca gcagcaggta
     5821 gcgctcccgg tagaggggga cggtccgcac cgcgccgcca agcggctccc cgtcgacgta
     5881 ggtcatgccc acgtcgagct cgtagtccgc cagcttgcgc acgatctcgc gcgatgacat
     5941 cgactcgaag gagatccgca cccgcgcgtg acgctcgctg aacggggcgg tgagcagggg
     6001 agccgcggtc agtgccgtgg ggatcgcgcc gatgcggagt ttgccggtga gaccgcccgc
     6061 catccggtcc aggtcctgcc gaagcgcgtc ctggtcggcg agcatccggt gcgcccagac
     6121 cacaacccgc tcgccctccg gggtgaaccc cccgaagcgg cgctcccgcc ggacgatctg
     6181 caccccgagg tccgcctcca gtcgccggat cgccgcagac agcgcaggtt gcgacacatg
     6241 gcacgcggca gcggcccgcg cgaaatgtcg ttcccgcgcg agagcactga gatattccag
     6301 ctggcgcagg agcaccggac aggaataccg gcgccacggc cgaaaggcaa agccgcgccg
     6361 tgccgggagc ggtcgcgacg acgccggcag cacgggtccc catccggcga ggcgtggcgc
     6421 tcaggtgggt ggcctgcggg tgatgtgtgc caccggcatc gatacggcaa ttgccgatat
     6481 gttgcaggag cgttctcggt gaaaggtgtg tcatgacatc ccggacagtt cctgcggcgc
     6541 catcgcggtg ggcgcagctg cgtacgacgc tcacccgtga ggatcgggca gcaatcggcg
     6601 gaatggccgg tttcgtcgtt ctcctgcatg ttgtcggatt cggcgtgctg atcgccgtcg
     6661 tcgttcctgc gcactacgac cttggcagca ccggcacgta cgggatcggg gtcggagtac
     6721 tggcgtacac gctgggcatg cgacacgcgt tcgacgccga ccacatcgcg gcgatcgaca
     6781 acaccacgcg caagctcatc gccgacggag gcaggcggcc cctgtcggtc gggttctggt
     6841 tctccctcgg ccattccacc gtcgtcttcg ggctgacgtt cctgctcgcc ttcggggtga
     6901 aggcgctggt cggggaggtc gagaacggcg gatcgcggct gcattcgatc ggcgggacca
     6961 tcggtgccac ggtttccggg acgttcctgt ggatcctggg gatcctcaat ctcgtcgtgc
     7021 tgatcgggat catccggatc ttccggagga tgcgggccgg agagtacgac gagcaggagc
     7081 tcgaggaaca gctcaacaag cgcgggttca tgaaccggtt cctcagcggg ctgacccggt
     7141 cgatcgcgaa accatggcac atctacccgg tcggggtgct cttcgggctg gggttcgaca
     7201 ccgccaccga ggtcggactc ctcgtgctgg cgggcggcgc ggcagcgttc agtctgccgt
     7261 tctacgcgat cctcgtgctc ccgatcctgt tcgcggccgg catgagcctg ctcgactccc
     7321 tcgatggcgt gttcatgaac tttgcctaca gctgggcatt cgccaggccg gtccgcaagg
     7381 tgttctacaa catcacgatc acggcgctgt ccatcgcggt cgctctggtc atcgggacga
     7441 tcgaactggt gtcggtgatc acccagcagg cggggatcga ctccgggccg ctcgcggccg
     7501 tcgcctcgat cccgctgaac gacgccgggt acggcatcgt cgcgctcttc gccctggcgt
     7561 ggcttgtcgc gttcgcgatc tggaggctcg ggcacgtcga gcagagatgg tccgcgacgc
     7621 cgggggagcc cggcgcggcc accggcacgg gcggcgaggc ccctgagacc gtggcctgac
     7681 cggcgggaga cgcctcagtc cgggtacgcc ccgccgtcga gcccgatgac cttgttggtc
     7741 aggtagccgt tgcggagcat cgcgagcgag aggtcggcca cctcctcggt ggtgcccagg
     7801 cggccggcgg gaattcgtgc gggaaccggc tcgtccggcc cgagcggcag catccgggtg
     7861 ccggcgatca gggccggggc gatcgcgttg atggtgacgc cggaaccggc aacgttcgcc
     7921 gagaggttgt gcacgaggcc gtgcagcgcc gccttggccg ccgcg
//
LOCUS       DASRSC010000106         1813 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2490718,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000106 DASRSC010000000
VERSION     DASRSC010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2490718"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="VFE39_05100"
     CDS             <1..718
                     /locus_tag="VFE39_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HET6258011.1"
                     /translation="SWTPFVGMFIARISRGRTIRQFVTGVILIPSLVSLIWFAIFGGA
                     AINLQRSGTDLASLPTEAQLFGLLDTLPLGGVLSVIAIVLIAIFFVSGADAAAVVMGT
                     LSQRGSIAPSRWVVVFWGVVMGAIAIIMLLVGDEGEALTGIQNITIIMAAPFAVVMVV
                     LCVALAKDLRNDPLMLRDRRSAAAVEQAVEYGTKNYGDEFVVSVKPHPGVTEDPSGPP
                     AEAAGGSNGAAGDGVPPART"
     gene            complement(776..1744)
                     /locus_tag="VFE39_05105"
     CDS             complement(776..1744)
                     /locus_tag="VFE39_05105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HET6258012.1"
                     /translation="MRAVQVVRLDGPAAVEVRDVPEPERRRDQVLVEVRAAGVNFPDV
                     LQTKGLYQYKPDLPFTLGSEVTGVVRDAPDGSPLRAGDRVAAFASTGAFAEVAAVQAD
                     HVLPLPDEVSFAKGACLPMNYLTAHFALLVRGHLRKGQTVLVHGAAGGVGTACVQLAA
                     ALGARVVAVVSTEEKAAAARAVGAHETVLAEGFRDAVKELTGGAGVDLVVDPVGGDRF
                     TDSLRSLAPEGRLLVVGFTGGDIPTVRVNRLLLNNIDVVGVGWGAFALARPGYLAGQW
                     ADLEPHLRSGALDPLMSATFPLERAAEALARIDERQVTGKVVLETS"
BASE COUNT          261 a          682 c          623 g          247 t
ORIGIN      
        1 ctcctggacc cccttcgtcg gcatgttcat cgcccggatc agccgcggtc gcacgatcag
       61 gcagttcgtc acgggcgtca tcctgatccc gagcctcgtc agcctgatct ggttcgccat
      121 cttcggcggc gccgccatca atctgcagcg cagcggcacc gacctcgcct ccctgcccac
      181 ggaggcccag ttgttcgggc tgctcgacac gctgccgctc gggggcgtac tcagcgtcat
      241 cgcgatagtg ctgatcgcga tcttcttcgt gtccggcgcc gacgcggcag cggtggtgat
      301 gggcacgctc tcgcagcgcg gctcgatcgc cccgtcccgc tgggtggtgg tgttctgggg
      361 agtcgtgatg ggcgcgatcg cgatcatcat gctgctcgtg ggcgacgagg gtgaggcgct
      421 caccggcatc cagaacatca cgatcatcat ggccgcgccg ttcgcggtgg tgatggtggt
      481 gctgtgcgtg gcgctggcga aggacctgcg caacgacccg ctcatgctgc gcgaccggcg
      541 ttccgcggcg gccgtcgagc aggcggtcga gtacgggacg aagaactacg gcgacgagtt
      601 cgtggtctcg gtgaagccgc accccggcgt gacggaggac ccgagcggcc caccggccga
      661 ggccgcgggg ggcagcaacg gggcggcggg cgacggcgtg ccacccgccc gcacctgaca
      721 gggcagaacg gccgcggggc ccgagcaggg tcccgcggcc gcatgttcag gtggttcagg
      781 aggtctcgag cacgaccttg cccgtgacct gccgctcgtc gatccgggcc agcgcctcgg
      841 cagcccgctc cagggggaag gtggcggaca tcagcgggtc cagcgcaccc gagcgcaggt
      901 gcggctccag atccgcccac tgcccggcca ggtagccggg ccgcgcgagc gcgaaagcgc
      961 cccagccgac gccgaccacg tcgatgttgt tcagcagcag ccggttcacg cgcacggtcg
     1021 ggatgtcgcc gccggtgaaa ccgacgacga gcagcctgcc ctccggcgcg aggctgcgca
     1081 gcgagtcggt gaaccggtca ccgcccaccg ggtcgacgac gaggtcgaca ccggccccgc
     1141 cggtgagctc cttcaccgcg tcgcggaacc cctcggcgag gacggtctcg tgcgccccca
     1201 ccgcccgcgc ggccgcggcc ttctcctccg tggagaccac cgcgaccacc cgggcaccca
     1261 gcgcggcagc aagctggacg cacgcggtgc cgaccccgcc ggccgccccg tgcacgagca
     1321 ccgtctggcc cttccgcagg tgcccgcgca cgagcaacgc gaagtgcgcg gtcaggtagt
     1381 tcatcggcag gcaggcgccc ttggcgaagg acacctcgtc cggcagcggg aggacgtggt
     1441 cggcctgcac cgcggcgacc tcggcgaatg cgccagtgct ggcgaacgcc gcgacacggt
     1501 cgcccgcacg cagcggcgac ccgtcgggtg catcacgaac gacgccggtc acctcggagc
     1561 cgagagtgaa cggcaggtcg ggcttgtact ggtagaggcc cttggtctgc agcacgtcgg
     1621 ggaagttcac cccggcggca cgcacctcca ccagcacctg atccctccgc cgctccggct
     1681 ccggcacgtc ccgcacctcg acagccgccg ggccgtccaa ccgcaccacc tgcaccgcgc
     1741 gcacttcgat ccctctccgt cgagcgtcac cggcccccat cctcacccat gcaggcgccg
     1801 tagccgctcc ccc
//
LOCUS       DASRSC010000107         1914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2163336,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000107 DASRSC010000000
VERSION     DASRSC010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2163336"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VFE39_05110"
     CDS             <1..240
                     /locus_tag="VFE39_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6258013.1"
                     /translation="VLVVGHNTALRLALCAWLGIPVSRYRDVLPRLDNASITQLRVPP
                     EPSQRPTLLSLNVPAVAVPPAAVPTAPVPIDWSRW"
     gene            237..659
                     /locus_tag="VFE39_05115"
     CDS             237..659
                     /locus_tag="VFE39_05115"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6258014.1"
                     /translation="MIGIPAAYPAGRWQYRGSNDVMFTILSFRFAPELMVIVPLYTRR
                     RAFMMVALPLVRPGIAASALLAFVFAWNNYVFPLILTGSRHTPVTVAVTRFLGGGGQA
                     YYNLTAAAALIAALPPLLVALSIQRYLVRNLSFGAVKA"
     gene            659..1786
                     /locus_tag="VFE39_05120"
     CDS             659..1786
                     /locus_tag="VFE39_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6258015.1"
                     /translation="MGSLAIRGLTARYGKTVALHGIDLELTDREFFVVLGPSGAGKTT
                     TLKSVAGLVDGAAGEVRIAGRDMTGVEPYDRGVAMAFESYALYPQRTVEQNLASPLRS
                     GRNGTWTPAQQAERIRQVTTTLGIDHLLGRYPRELSNGQRQRTALGRVLVRPAEVYLL
                     DEPLSHLDAKLRAAMRAELKQLGDLSGTTTLYVTHDYQEALALGDRIGVLREGRLVQV
                     GTPTQVWTEPVDTFVARTIGQPEMNLLDAVVDGGAARTPGGGVVVGVGPEVPAGDAVR
                     LGIRPRDLEIVDRQAPDDRLVLRGRVVLAERLGRSVELSIDVAGVQVIAVTADHRTRE
                     GDSVTLVADWANVHVFGAGEPAPRLGAACRPEPAQLAGGAR"
BASE COUNT          252 a          678 c          708 g          276 t
ORIGIN      
        1 gtgctggtcg tcgggcacaa caccgcgctg cgcctggccc tgtgcgcctg gctcggcatc
       61 cccgtttcgc gctaccgcga cgtgctgccg cggctggaca acgcgagcat cacccagttg
      121 cgcgtgccgc ccgagccgtc gcagcggccc acgctgctga gcctcaatgt cccggcggtc
      181 gccgtcccgc ccgccgccgt cccgaccgcc ccggtcccca tcgactggtc tcgctggtga
      241 tcggcatccc cgccgcctac cccgcgggac gctggcagta tcgcggctcg aacgacgtga
      301 tgttcacgat cctgtcgttc cggttcgcgc cggagctgat ggtgatcgtg ccgctgtaca
      361 cccgccgccg cgcgttcatg atggtggcgc tgccgctcgt gcggccgggg atcgctgcgt
      421 cggcgctgct cgcgttcgtc ttcgcgtgga acaactatgt gttcccgctg atcctcaccg
      481 ggagccggca cacgccggtg acggtggcgg tcacccgctt cctcggcggg ggcggccagg
      541 cctactacaa cctgacggcc gccgcggcgc tgatcgccgc cctcccgccg ctgctcgtcg
      601 cgctgtcgat ccagcgctac ctggtgcgga acctgtcctt cggggcggtg aaggcgtagt
      661 gggttcgctc gccatccggg ggctcacggc gcgctacggg aagaccgtcg ccctgcacgg
      721 gatcgacctc gagctcaccg accgagagtt cttcgtggtc ctcggtccgt ccggggcggg
      781 gaagaccacc acgctcaaga gcgtggccgg gctcgtcgac ggagcagcgg gggaggtgcg
      841 catcgccggg cgcgacatga cgggggtcga gccctacgac cgcggtgtcg cgatggcgtt
      901 cgagagctac gcgctctacc cacagcgcac ggtggaacag aacctcgcct cgccgctgcg
      961 gtcaggccgc aacggaacgt ggacgcctgc gcagcaggcc gagcggatcc ggcaggtcac
     1021 cacgacgctg ggcatcgacc acctgctggg gcggtacccg cgtgagctgt ccaacgggca
     1081 gcgacagcgc acggcgctcg gccgggtgct ggtgcggccc gccgaggtgt acctgctcga
     1141 cgagccgttg tcgcacctcg acgccaagct gagggccgcg atgcgggcgg agctgaagca
     1201 gcttggcgac ctctcgggca cgacgacgct gtacgtcacg cacgactacc aggaggcgct
     1261 cgcactgggc gaccggatcg gggtgctgcg cgagggcagg ctggtacagg tcgggacgcc
     1321 cacgcaggta tggaccgagc cggtcgacac gttcgtggcg cgcacgatcg gccagccgga
     1381 gatgaacctc ctcgacgcgg tggtcgacgg cggggcagcc cgcacccccg gcggtggtgt
     1441 cgtggtgggg gtggggcccg aggtgccggc gggcgacgcc gtccggctcg ggatccggcc
     1501 ccgcgacctg gagatcgtcg acaggcaggc cccggacgac cggctggtgc tgcgcgggcg
     1561 ggtggtgctg gccgagcgac tgggccggtc cgtggagctc agtatcgacg tcgcgggggt
     1621 gcaggtcatc gccgtcacgg ccgaccatcg gacgcgggag ggtgactccg tcactctcgt
     1681 ggccgactgg gcgaacgtgc acgtcttcgg tgcgggggag cctgccccgc ggctcggcgc
     1741 cgcgtgcagg cccgagcccg cccagctcgc aggaggagcc cgatgaccgc caccgtcgac
     1801 gcccccccgc aggcacagcg gctgcggctg gaggccctgc gcaagacgta caccgcccgc
     1861 ggccgagagc cgttcgaggc cgtgaagggg atcgatctgg acatcgagcc gggt
//
LOCUS       DASRSC010000108        10248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4128205,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000108 DASRSC010000000
VERSION     DASRSC010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4128205"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..1106
                     /locus_tag="VFE39_05125"
     CDS             144..1106
                     /locus_tag="VFE39_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HET6258016.1"
                     /translation="MPVYADQRPYPTITVLHAEDRPPGLTRDRIRFATDHTLAHALPG
                     TEVLLVWDFTSDAVREAWPAADALRWVHTASAGVDRLVFPELLDSPVTLTNSRGVFDV
                     PMAEYVLGLVLAMAKDLPGTLALQSRREWRHRESERVAGTCAVVVGGGPIGRATARLL
                     SAVGMRVELVGRQASDGVHAAEALPGLLPLGDWLVLAAPLTEATRGMLDAAALAALRP
                     SARVINVGRGALVVEPDLVDALRGGRLAGAALDVFAQEPLPPESPLWDLPGVIVSPHM
                     SGDVLGWREELVEVFADNLARYRAGEPLLNVVDKTRGYVTGGTS"
     gene            1103..2563
                     /locus_tag="VFE39_05130"
     CDS             1103..2563
                     /locus_tag="VFE39_05130"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET6258017.1"
                     /translation="MTGTTTGAGAVGDGAIGDGARSAADLTATELLAAYRTKELSPVE
                     ATRAVLDRVAERDGEVNAFCLVDADTALAGAARSEGRWARGEPVGLLDGVPVSIKDVL
                     LTTGWPTLRGSRTVDPAGPWTVDGPPVARVRGHNGVLFGKTTTPDLGWKGVTDSPLTG
                     VTRNPWDPTRTAGGSSGGSAAAVLLGMGPLSLGTDGGGSVRIPAGFSGVTAHKPTYGR
                     VPHHPASPFGTLAHVGPMARTAADAALLLDVVSGTDARDPWALAPTGSAVAALGAPVA
                     GLRIAASPTLGYVDVHPEVAAAFAAAVEVFRAMDADVDEADPGFGDPVEAFELLWFAG
                     AAKSIENLGPAERAVLDPGLRESAEQVAEATALDYLEAMGVRNALGTLMGEFHSRYDL
                     LLTPTLPIPAFEAGVEVPAGWPRKRWTSWTPFTYPFNMTQQPAASVPCGFTSRGLPVG
                     LQIVGPRHADNAVLAAAHAFQQATSWHLRQPSTDRP"
     gene            complement(2574..2984)
                     /gene="tatB"
                     /locus_tag="VFE39_05135"
     CDS             complement(2574..2984)
                     /gene="tatB"
                     /locus_tag="VFE39_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HET6258018.1"
                     /translation="MFDSVGWGEIVVLVVAGLFILGPERLPSAASWVGRSIRQVREYA
                     NGAREQLRGELGPEFDELRKPLEELRGLRDFNPRNAVRRTLFDDPAPAKPNGFAPGPN
                     LTKSNDSPQQHPAAPPAPETLARNERPPIDPDAT"
     gene            complement(3078..4466)
                     /locus_tag="VFE39_05140"
     CDS             complement(3078..4466)
                     /locus_tag="VFE39_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6258019.1"
                     /translation="MEGGFAAPRGGTPTPTPATVAAPPPAALASAFGRPVADAPPLQR
                     PPTNGAGGDDVDGAFWVEGADRDPWRDPDSAVELGPPAAADRPVRSRPRGEGARLSLR
                     ELVFGRRVDPRALAGLALVAVLIGAAGGLVGRLTADGAAGLTDPDPTITQAVAAKERP
                     PGSVADIAARTVPAVVSIEVRVGQEGGTGSGVVIDPAGYVLTNNHVVAPAAEATGAQI
                     EAIFHDGTRSPAQIVGRDPKTDLAVVKVKVANPVVATIGSSAGLAVGDGVLAIGSPLG
                     LVGTVTDGIVSALNRPVRLDGSGTDASAVIDAIQTDAAINPGNSGGPLVDSTGAVVGI
                     NTAIRSLGAGEGGSIGLGFAIPIDNARAIAEELIRTGVVTHAEIGINARSVNDGTTDG
                     AQVQNVQQGGAAAAGGIVEGDVIVRVGNRPIAGADELVVAVRERNPGDTVPVELIRDG
                     RQLTLSVVLTSD"
     gene            complement(4693..5466)
                     /locus_tag="VFE39_05145"
     CDS             complement(4693..5466)
                     /locus_tag="VFE39_05145"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6258020.1"
                     /translation="MSDGRWRITVSPPDWGQDHLSSDAIVAYVDDELDHGPQERAAQH
                     ISQCAECEAQIVAQVQTRSALRAAEGPVLPSTLLRNLRSIPESAELPPPPPGLAMTPE
                     GQLVSVLRPGPDSRPVPNGADLRTGGAGTPRGVAAPRSPRHGSLPERSPVQRRIRVGT
                     GVAVSGLALGALAFGLPTATDPTSPAVSGRGVLGGSLLGGTGVADTATASGTGTGAGL
                     VDAQLRLERRVPLARDPEAQPDMVEGRLDLRPGSFYGLP"
     gene            complement(5463..6092)
                     /gene="sigE"
                     /locus_tag="VFE39_05150"
     CDS             complement(5463..6092)
                     /gene="sigE"
                     /locus_tag="VFE39_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigE"
                     /protein_id="HET6258021.1"
                     /translation="MTDGSTTLLSGTVLPGEGAEWTPPSWDDIVREHADRVYRLAYRL
                     TGNPHDAEDLTQETFVRVFRSLASYKPGTFEGWLHRITTNLFLDMVRRRSRIRMESLP
                     EDSDRLPGLGPEPEQVFSETHLDPVLQAALDELPPEFRAAVVLCDVEGLSYEEIGATL
                     GVKLGTVRSRIHRGRAALRNALERHRALEVHGVAAAKASADVLLEEVHG"
     gene            6332..6991
                     /locus_tag="VFE39_05155"
     CDS             6332..6991
                     /locus_tag="VFE39_05155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6258022.1"
                     /translation="MTGRPDSASDPLASRDNVEGYLPEDDVLTAARASSAQRGCAPVS
                     PAAGAGLRFLAGTIAARAVVEVGTGVGVSGLWLLGGMAADGVLTSIDIDPEHQRAARQ
                     AFTAAGYGAPRLRLINGMGLEVLPRLTDGGYDLVFVDAGAADYPRYLDEAVRLLRGGG
                     IIALHGVLTDGVLNPDVSDAGSVALREVARQAREDDRLIPVLLPLGGGLLAAVKRTTS
                     G"
     gene            complement(7008..8258)
                     /gene="glgC"
                     /locus_tag="VFE39_05160"
     CDS             complement(7008..8258)
                     /gene="glgC"
                     /locus_tag="VFE39_05160"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009076484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET6258023.1"
                     /translation="MTAARLSPASPRAMAANVLGIVLAGGEGKRLWPLTADRAKPAVP
                     FGGNYRLIDFVLSNLVNAGMHRICVLTQYKSHSLDRHISTTWRLSSVLGQYITTVPAQ
                     QRLGRRWYTGSADAIFQSLNLVYDERPDYIAVFGADHVYRMDPGQMLARHIESGAGVT
                     VAGIRVPRAEAKAFGVINSDADGRIIDFLEKPSDPPAVPDDPDVTFASMGNYIFTTEV
                     LLDALRADAEDGDSDHDMGGDIIPRLVGQGDAAVYDFADNVVPGATDRDSGYWRDVGT
                     VDAYYEAHTDLVSVHPIFNLYNDRWPIRTAMAPLPPAKFVEGGIAQDSIVGAGTIISG
                     AIVRRSVISPNVRIQAGAEVSDSVVLPGARVGRGAVVRGAILDKNVVIPDGALVGVDL
                     GLDRARYTVSQGGVVVLGKGATAL"
     gene            8432..9601
                     /gene="glgA"
                     /locus_tag="VFE39_05165"
     CDS             8432..9601
                     /gene="glgA"
                     /locus_tag="VFE39_05165"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HET6258024.1"
                     /translation="MRIGLLTREYPPDVYGGAGVHVEHLVPALREFVDVNVHCFGEPR
                     PDQPDTHAHVPPTALAEANPALQTLGVDLSMVAALAAPPAVDLVHSHTWYANMAGHLA
                     KILHGVPHVVTAHSLEPRRPWKAEQLGGGYRLSSWVERTSYEHADAVIAVSEGMRRDV
                     LDCYPALDPARVHVVHNGIDTAFYHPDPARDAVHAAGIDPGRPSVVFVGRITRQKGLG
                     HLVAAAHRIDCDAQIVLCAGAPDTPQIAEETEKAVAELSATRPGVVWVRRMLPTAEVR
                     QLLSAATVFVCPSVYEPLGIVNLEAMACGTAVVASDVGGIPEVVADGETGLLVHFDEH
                     AVEEFRTGLADAVNALLADPARAMAMGAAGRVRAEREFAWEQAAARTVEIYRALR"
     gene            9692..9838
                     /locus_tag="VFE39_05170"
     CDS             9692..9838
                     /locus_tag="VFE39_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258025.1"
                     /translation="MLSTHHTSEGWVRYRRCVCGTMSIQLVTLEGPTHTQAEAVPESV
                     TTPY"
     gene            complement(10023..10190)
                     /locus_tag="VFE39_05175"
     CDS             complement(10023..10190)
                     /locus_tag="VFE39_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018254362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3117 domain-containing protein"
                     /protein_id="HET6258026.1"
                     /translation="MAAMKPRTGDGPLEVTKEGRGIVMRVPLEGGGRLVVEMSAEEAS
                     DLGDALKAASG"
BASE COUNT         1390 a         3669 c         3758 g         1431 t
ORIGIN      
        1 ggcgggccag cgtgatgcgg aggaacttcg acatgcacga ctcccatccg acggatcccg
       61 cagcaccgcg gattgttgac aatcatacga agccttccta ccgtgtcaac actcggcccc
      121 cgctccggaa ggttgcgtcc cacttgcccg tttacgcaga tcagcggccg tatcccacga
      181 tcaccgtgct gcacgcggag gaccggccgc ccggcctgac tcgtgaccgg attcgcttcg
      241 ccaccgacca cacgttggca cacgcgctgc ccggcaccga ggtgctgctc gtctgggact
      301 tcacctcgga tgccgtgcgc gaggcgtggc ccgccgccga cgcgctgcgc tgggtgcaca
      361 ccgcgagcgc gggggtcgac cggctggtct tccccgagct gctggactcg ccggtgacgc
      421 tgacgaactc ccgaggcgtg ttcgacgtgc ccatggccga gtacgtgctc gggctcgtgc
      481 tggccatggc gaaggacctg cccggcaccc tcgccctgca gtcgcggcgg gagtggcggc
      541 accgtgagag cgagcgggtg gccggcacgt gcgccgtggt ggtgggcggc ggaccgatcg
      601 ggcgagccac cgcgcgcctc ctgagtgccg tggggatgcg ggtggagctg gtcgggcgcc
      661 aggcgtcgga cggcgtccac gcggccgaag cgcttcccgg tctcctgccc ctcggcgact
      721 ggctcgtgct cgccgcgccg ctcaccgaag ccacccgcgg gatgctggac gcggcggcgc
      781 tcgccgcgct gcgcccgtcc gcccgcgtga tcaacgtggg acgcggcgcg ctcgtcgtcg
      841 aacccgacct cgtcgacgcg ctgcgtggcg gccgcctcgc cggggcggct ctcgacgtgt
      901 tcgcccagga gccgctcccc ccggagagcc cgctgtggga cctgcccggc gtgatcgtgt
      961 cgccacacat gtccggcgac gtcctcggct ggcgggagga gctcgtcgag gtgttcgccg
     1021 acaacctcgc ccgctaccgc gcgggggagc cgctgctcaa cgtcgtcgac aagacgcggg
     1081 gctacgtcac cggagggacc tcatgaccgg cacgaccacc ggagccggcg ccgtcggcga
     1141 cggcgcgatc ggggacggtg cgcgctccgc cgccgacctc accgccaccg aactgctcgc
     1201 cgcctaccgg accaaggagc tctcgccagt cgaagccacg agggcggtgc tcgaccgagt
     1261 ggcggagcgg gatggcgagg tcaacgcgtt ctgcctggtc gacgcggaca cggcgcttgc
     1321 cggcgcggcc cggtccgagg ggcggtgggc acgcggcgag ccggtggggc ttctcgacgg
     1381 cgtaccggtc tcgatcaagg acgtcctcct caccaccggc tggccgacgc tgcgcggctc
     1441 ccgcacggtc gaccccgccg gcccgtggac cgtcgacggt ccaccggtag ccagggtgcg
     1501 cggccacaac ggcgtcctgt tcggcaagac caccaccccc gatctcgggt ggaagggcgt
     1561 caccgacagc ccgctgaccg gcgtgacccg caacccgtgg gatccgaccc gcaccgcggg
     1621 tggctcctcg ggcggcagcg cggccgcggt cctgctcggg atgggccccc tcagtctggg
     1681 caccgacggc ggcggctcgg tgcggatccc ggccgggttc tccggcgtca ccgcacacaa
     1741 gcccacctac ggcagggtgc cgcaccaccc ggcgagcccg ttcggcacgc tcgcgcacgt
     1801 ggggccgatg gcccgcaccg cggccgacgc cgcgctgttg ctggacgtcg tctccggcac
     1861 agacgcccgt gacccgtggg cgctggcacc caccggctcg gcggttgccg cgctgggagc
     1921 tccggtcgcc ggtctgcgga tcgccgccag ccccactctc gggtacgtcg atgtgcaccc
     1981 cgaggtggcg gcggcgttcg ccgccgccgt cgaggtgttc cgcgcgatgg acgccgacgt
     2041 cgacgaggcc gacccggggt tcggtgaccc ggtggaggcg ttcgagctgc tgtggttcgc
     2101 cggcgccgcc aagtcgatcg agaatctggg gccggccgag cgcgcggtgc tggacccggg
     2161 cctgcgcgag agcgccgaac aggtggccga ggccaccgcg ctcgactatc tggaagccat
     2221 gggagtgcgc aatgcgctcg gtaccctgat gggcgagttc cactcccgct acgacctgct
     2281 actcaccccg acgctgccga tccccgcgtt cgaggcgggg gtggaggtgc cggctgggtg
     2341 gccgcggaag cgctggacgt catggacgcc gttcacctac ccgttcaaca tgacccagca
     2401 gcctgcggcg agcgtgccgt gcgggttcac gtcacggggt ctgcccgtcg ggctgcagat
     2461 cgtggggccc cggcacgccg acaacgcggt gctggcagcc gcgcacgcgt tccagcaggc
     2521 caccagctgg catctgcggc agccgtccac ggaccgaccc taggagcgcg ctcctaggtg
     2581 gcgtcggggt cgatcggcgg ccgctcgttc ctggccaggg tctccggggc cggaggggcc
     2641 gccgggtgct gctgcggcga atcgttgctc ttcgtcaggt tcggaccggg agcgaagccg
     2701 ttgggcttcg ccggcgccgg gtcgtcgaag agggtgcgcc gcaccgcgtt gcgcgggttg
     2761 aagtcccgca gcccgcggag ctcctccagc ggcttgcgca gctcgtcgaa ctccgggccg
     2821 agctcgccgc ggagctgctc gcgcgccccg ttggcgtact ccctcacctg ccggatagat
     2881 cggcccaccc atgaggcagc agacggaagc cgctcgggac cgaggatgaa cagcccggcc
     2941 acgaccaaca cgacgatctc gccccagccg acgctgtcga acacgacctc atcctacggg
     3001 gcgacacgcg ccatgaggtc ctcggcaccg cccggccagc gagatcggac cgccggcgcc
     3061 cggtatttcc gaggtgttca gtcggaggtc aggaccacag acagcgtgag ctgcctgccg
     3121 tcccggatca gttcgaccgg cactgtgtcg cccgggttgc gctcgcggac cgcgaccacg
     3181 agttcatccg cacccgcgat cggccgattc ccgacccgga cgatcacgtc tccctccacg
     3241 atcccgcctg ccgcggcggc cccgccctgc tgcacgttct gcacctgggc gccgtcggtg
     3301 gtgccgtcgt tgaccgaccg ggcgttgatc ccgatctcgg cgtgggtgac gacgccggtg
     3361 cggatcagct cctcggcgat ggcccgcgcg ttgtcgatgg gaatggcgaa gccgaggccg
     3421 atcgagccac cctcaccggc cccgaggctg cggatggccg tgttgatccc cacgaccgca
     3481 ccggtggagt cgacgagcgg accacccgag ttgcccgggt tgatcgccgc gtccgtctgg
     3541 atcgcgtcga tcaccgcgct cgcgtcggtg ccgctgccgt cgagccggac cggccggttg
     3601 agcgcgctga cgatgccatc ggtgaccgtg ccgacgaggc ccagcggtga accgatggcg
     3661 agtacgccgt cgccgacggc gagcccggca gaggagccga tggtggcgac caccgggttc
     3721 gcgaccttca ccttgacgac ggccagatcg gtcttcgggt cgcggcccac gatctgcgcg
     3781 gggctgcgcg tgccgtcgtg gaagatggcc tcgatctgcg cgccggtggc ttcggcggcg
     3841 ggggcgacca cgtggttgtt cgtcaggacg tagccggcag ggtcgatgac gacgcccgag
     3901 cccgtcccgc cctcctggcc gaccctgacc tcgatcgaga cgacagccgg gacggtgcgg
     3961 gcggcgatgt cggccaccga gccgggtggg cgttccttcg ccgcgacggc ctgcgtgatc
     4021 gtcgggtcgg gatcggtgag cccggcggcg ccgtcggccg tcagccgccc gacgagcccg
     4081 cctgcagcgc cgatcagcac cgcgaccagg gcgagcccgg ccagagctcg cggatcgacc
     4141 cgccggccga agaccagctc ccgcaggctg agccgcgcgc cctcaccacg tggccgggac
     4201 cgaaccggcc ggtccgcggc ggcgggcgga ccgagttcga cggcactgtc cgggtcgcgc
     4261 cacgggtcgc ggtcggcacc ctcgacccag aaggccccat caacgtcgtc gccacccgct
     4321 ccgttggtcg ggggccgctg caacggcggt gcatcggcaa cggggcgacc gaaggcgctg
     4381 gccagcgctg cgggcggtgg tgcggcgacg gtggcggggg taggggtcgg cgttccgcca
     4441 cgcggggcgg cgaagccgcc ctccacgccg ctcggtcggc cgaaaacctc agctgtgtct
     4501 gggtcgacgg gcggccggtc caacgggcgc ggaccgagcc gcggcgggtc agcgctcgcg
     4561 ccatcgttcg aacgcgtggc gggggcgctg cgttcgttca tcgcgctgga ggctcctggg
     4621 gtgggcggac cataccgctg gccagcctgc cccagcggtc gtgaagtccg cgtagccggg
     4681 cttgcgaccc gctcagggca gaccgtagaa ggagccgggt cgcaggtcga gccggccctc
     4741 gaccatgtcg ggctgagcct cggggtcgcg cgcgagcggg accctccgct ccagtcggag
     4801 ctgggcgtcg accaggccgg caccggtgcc ggtgccgctg gcggtggcgg tgtctgcgac
     4861 gccggtaccg ccgagcagcg agccgccgag cacaccgcgg ccggatacgg ccggggatgt
     4921 cgggtcggtc gcggtgggca gaccgaaggc gagcgcaccg agcgcgagcc cggacacagc
     4981 cacgcccgtg ccgacgcgga tgcgtcgttg caccggggac cgttccggaa ggctgccgtg
     5041 gcgggggctc cgcggtgcgg ccacgccacg cggggtaccg gcgccgccgg tgcggaggtc
     5101 ggccccgttc gggaccggtc gggagtcagg cccgggacgg agcacggaga cgagctgtcc
     5161 ctcgggggtc atcgcgagcc ccggcggcgg aggcggcagc tcggcgctct ccggaatcga
     5221 gcggaggttg cggaggagcg tcgagggcag cacgggcccc tccgcggcgc gcagggcgga
     5281 gcgggtctgt acctgggcga cgatctgcgc ctcgcactcg gcgcactggg agatgtgctg
     5341 ggcggctcgc tcctgcggcc cgtggtccag ctcgtcgtcc acgtaggcga cgatggcgtc
     5401 ggacgacagg tgatcctgcc cccagtcagg ggggctcacc gtgatgcgcc agcgcccgtc
     5461 gctcacccgt gaacctcctc cagcaacacg tcggcggaag ccttcgcggc cgcgacgccg
     5521 tgcacctcaa gcgcacggtg gcgctccagc gcgttgcgca gagccgcccg tccgcggtgg
     5581 atcctgcttc tcaccgtacc cagcttcacg ccgagggtgg cgccgatctc ctcgtaggac
     5641 aacccctcca cgtcgcacag gacgaccgcg gcgcggaact cgggaggcag ctcgtcaaga
     5701 gccgcctgca gcacggggtc gaggtgggtc tcggagaaca cctgctccgg ctccgggccg
     5761 aggccgggca gccggtcgga gtcctccggg agcgactcca tccgaatgcg ggagcggcgg
     5821 cggaccatgt ccaggaacaa gttggtggtg atgcggtgca accagccctc gaacgtgccg
     5881 ggcttgtagg aggccagcga ccggaacacc cggacgaacg tctcctgggt gaggtcctcg
     5941 gcgtcgtgtg ggttgccggt gaggcggtag gcgagacggt agacccggtc cgcgtgctcg
     6001 cggacgatgt cgtcccagct cggtggggtc cactcggccc cctcaccggg cagcaccgta
     6061 ccgctcagga gcgtggtgga gccgtcggtc atggcgggtc aaacctcctg cggcgggtcg
     6121 tgccctccga ccaacgaagg accggaggtt ggtgttcccg gcccatcagc ggccgacgac
     6181 cccgaccgat gcaacctacc gtgcctcatg gccgtgaacg tcgcgtgaga accgactgag
     6241 agaagcctgt gaaaccgggc acccacgtct gggaagggcc gatcctcggg cccacactgc
     6301 aggtgagcac cagcgcagta gcctcttgac catgaccggc cgcccggaca gcgcctccga
     6361 cccgttggcg tcgcgcgaca acgtcgaagg ttacctcccc gaggacgacg tgctcaccgc
     6421 cgccagagcc tcgtcggcgc agcgcggctg cgccccggtg agccccgcag cgggcgctgg
     6481 gctccgcttc ctcgccggca ccatcgcggc cagagcggtc gtcgaggtcg gcacgggcgt
     6541 gggcgtcagc ggcttgtggc tgctgggcgg gatggcggcc gacggcgtgc tcacctcgat
     6601 cgacatcgac ccggaacacc agcgcgccgc gcgccaggcg ttcaccgcgg ccgggtacgg
     6661 ggcgccccga ctccggttga tcaacgggat gggcctcgag gtgctgcccc ggctcaccga
     6721 cggtgggtac gacctggtgt tcgtcgacgc aggcgccgct gactacccgc gctacctcga
     6781 cgaggccgtg cggctgctgc gcggcggcgg catcatcgcg ctgcacggcg ttctcaccga
     6841 tggcgtcctc aacccggacg tctccgacgc cggctcggtc gcgctgcgcg aggtggcccg
     6901 ccaggcgcgg gaggacgacc gcctcatccc ggtactactc cccctcggcg gcggcctgct
     6961 ggcagcggtc aagcgcacga catccgggtg aggcaggaac gcctgcgcta cagggcggtg
     7021 gcgcccttgc ccagcacgac gacgccgccc tggctcaccg tgtagcgggc ccggtcgagg
     7081 ccgaggtcca cgccgacgag tgcgccgtcc ggtatgacga cgttcttgtc caggatcgcg
     7141 ccgcgcacca cggcgccgcg cccgaccctg gccccgggca ggaccaccga gtcggacacc
     7201 tcggcaccgg cctggatgcg cacgttcggg ctgatcaccg accgccgaac gatggcgccg
     7261 gagatgatcg tgccggcccc gacgatcgag tcctgggcga tgccaccctc cacgaacttg
     7321 gcaggcggca ggggcgccat cgccgtgcgg atgggccagc ggtcgttgta gaggttgaag
     7381 atcgggtgca ccgagacgag gtcggtgtgt gcctcgtagt aggcgtcgac ggtgccgacg
     7441 tcgcgccagt agccggagtc gcggtcggtg gcacccggca cgacgttgtc ggcgaagtcg
     7501 tacaccgcgg cgtcaccctg tccgaccagc ctcgggatga tgtcgccgcc catgtcgtgg
     7561 tcggagtcgc cgtcctcggc atcggcccgc agcgcgtcga gcagcacctc ggtggtgaag
     7621 atgtagttgc ccatcgacgc gaaggtgaca tcggggtcgt ccggtacggc gggcggatcg
     7681 gacggcttct cgaggaagtc gatgatccgg ccgtcggcgt cggagttgat cacgccgaaa
     7741 gccttggcct ccgcgcgcgg cacccggatg cccgccacgg ttacgcctgc gccgctctcg
     7801 atgtgccggg ccagcatctg gccggggtcc atccggtaca cgtggtcggc accgaagacc
     7861 gcgatgtagt ccggccgctc gtcgtagacg aggttgaggc tctggaagat ggcgtcggcg
     7921 ctgcccgtgt accagcgcct gccgaggcgc tgctgggccg ggacagtggt gatgtactgg
     7981 ccgagcacgc tggacaggcg ccacgtggtg gagatgtggc ggtccagcga gtgcgacttg
     8041 tactgcgtga gcacgcagat gcggtgcatc ccggcgttga ccaggttgga cagcacgaag
     8101 tcgatcaagc ggtagttgcc gccgaacggc accgcgggct tggcccggtc ggcggtgagt
     8161 ggccagagcc gcttgccctc gcctccggcg aggacgatgc cgaggacgtt ggcggccatc
     8221 gcacgcggcg aggcgggtga caggcgggcc gctgtcattc cggcgctccg atcgtgggca
     8281 ccgggccctg gcgtgccggc agcatcgtca ctctgccgac cctagtgctc tcctaccgtg
     8341 gcaggccacc ggaaccgagc gtcgaaccgg cgaactcacg catcggacgt accccccggc
     8401 gcgtcacaca tcgatgcctt accgtcgccg tgtgcgcatc ggcctgctca cccgcgagta
     8461 ccccccggac gtctacggcg gagccggcgt gcacgtcgag cacctcgtgc cggcgctgcg
     8521 cgaattcgtc gacgtcaacg tccactgctt cggcgagccg cggcccgacc agccggacac
     8581 ccatgcgcac gtccctccca ccgcgctggc cgaggcgaac ccggcgttgc agaccctcgg
     8641 cgtcgacctg tcgatggtcg ctgccctcgc cgctcccccc gccgtcgacc tggtccattc
     8701 ccacacctgg tacgcgaaca tggccggcca cctggccaag atcctgcacg gcgtgccgca
     8761 cgtggtgacc gcgcactcac tggagccgcg caggccgtgg aaggccgagc agctcggggg
     8821 cggataccgg ctctcctcat gggtggagcg cacctcctac gagcatgccg acgccgtgat
     8881 cgcggtgagc gaaggcatgc ggcgcgacgt cctcgactgc taccccgcgc tggatccggc
     8941 gcgcgtgcac gtggtgcaca acggcatcga caccgccttc taccaccccg acccggcgcg
     9001 ggacgccgtg cacgcggccg gcatcgaccc cgggcgcccg agcgtcgtgt tcgtcgggcg
     9061 catcacccgg cagaagggcc tcgggcacct cgtggcggcc gcccaccgga tcgactgcga
     9121 cgcccagatc gtgctgtgcg ccggggcgcc ggacaccccg cagatcgctg aggagaccga
     9181 gaaggccgtc gccgagctga gcgcgacgcg tccaggggtc gtgtgggtcc gccggatgct
     9241 gccgacggca gaggtgcgac agctgttgtc ggcggccacg gtgttcgtct gcccttcggt
     9301 gtacgagccg ctcgggatcg tgaacctgga ggcgatggcc tgcggtaccg cggtggtggc
     9361 ctccgacgtc ggaggcatcc ccgaagtcgt cgccgacggc gagacggggc tgctggtgca
     9421 cttcgacgag cacgcggtcg aggagttccg caccgggctc gctgacgcgg tgaacgcgct
     9481 gctggccgac ccggcccggg caatggcgat gggggcggcg ggccgggtgc gggcagagcg
     9541 ggagttcgcc tgggagcagg cggcagcgcg cacggtggag atctaccggg ccctgcgctg
     9601 agctgaccgg ggtcgtgcgc ggtaactagt gcgctggcac tcgttaccgc gcacgggtgc
     9661 cggaggcgcg cggcccgccg atgaggcgca ggtgctgtcc acccaccaca cgtcggaggg
     9721 gtgggtgcgc taccggcgtt gtgtctgcgg cacgatgtcg atccagctcg tcacgctgga
     9781 ggggccgacg catacccagg ccgaggccgt gcccgagtcc gtgaccaccc cgtattgatc
     9841 gacagttcgg ggattcgcag cagcacacga ccggccttcc gatgccgagc cgacgagcat
     9901 ccggcgagtg ccaggtcgcg caccacggac gggtcgaacg acgaacggca ccggcggacg
     9961 gcgggtgccg ttcgtcagtg ccgttcggga accgcaggcc ccgacgccgg ggctcgtact
    10021 cgtcagccgg aggcggcttt gagcgcgtca ccgaggtcgc tggcctcctc ggccgacatc
    10081 tccacgacca ggcggccgcc gccctcgagg gggacgcgca tcacgatgcc ccgaccctcc
    10141 ttggtcacct ccaggggacc gtcgccggtc cggggcttca tcgccgccat ctcctgctcc
    10201 ctccgtcgtc gtttgcaggg tgcgccgctg cgccggggag agcgcgcg
//
LOCUS       DASRSC010000109         9386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1016435,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000109 DASRSC010000000
VERSION     DASRSC010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016435"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VFE39_05180"
     CDS             complement(<1..682)
                     /locus_tag="VFE39_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258027.1"
                     /translation="MTKNARPRHATRSRTSRIAGRAALGVLGAPVALLAIAGPASAAE
                     ADLSGHYTDDETAGSMDAGLDNAQALPGFPGTDVAAFPTNGLPTDGLDLQGIDLGLLN
                     LGGGGAGLPQLPVGAPDLSALQLNLPAVDTLPSLPSTDALPSLPSTDALSSLPVVGDL
                     PSLPSLPSLPSTDALPSLPSLPVVGALPSLSSLPVVGDLPSLPSTDSLPSLPVVGSLP
                     SLPSLPSLP"
     gene            complement(904..2199)
                     /gene="purD"
                     /locus_tag="VFE39_05185"
     CDS             complement(904..2199)
                     /gene="purD"
                     /locus_tag="VFE39_05185"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET6258028.1"
                     /translation="MRVLVVGSGAREHAIVLAMAQDPEVTALACAPGNAGTAAVAEQR
                     GLDVLDPVAAAELAVEWKADLVVVGPEGPLVAGVADAVRGAGIACFGPSAEAARIEGS
                     KSFAKDVMAAAAVPTATSEIVDNPAHLDAALSRFAPPYVVKDDGLAAGKGVVVSSDLD
                     AARAHARTLLDDGHPVLLESYLDGPEVSLFCLVDGETVVPLLPAQDFKRVGDDDCGPN
                     TGGMGAYAPLPWASPALADELVATVVAPVAAEMVRRGTPFQGLLYAGLALTSAGPSVI
                     EFNCRFGDPETQVVLALLRTPLARLLHSAATGALVAHPPLEWSDGAAVTVVVAAEGYP
                     GLPRLGDVISGSEADGVLHAGTRRRDDGAVVSTSGRVVSVLGTGADLALARDDAYRRL
                     EGVHLAGSHHRTDIALRASRGEVTIPQGAEVVTRSGDVG"
     gene            2268..3428
                     /locus_tag="VFE39_05190"
     CDS             2268..3428
                     /locus_tag="VFE39_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET6258029.1"
                     /translation="MPFTGWTRNSHLPVAVGSLDRGKVGNVSSAFTASALTTAPTGAS
                     AAAGKAREKSGAHVLVIGHRGASGYRPEHTLASYELAARMGADFVEPDLVSTSDGVLV
                     ARHESEIGGTTDVANRPQFADRRTTKTIDGVLRTGWFTEDFTLAELKTLRAKERIPGF
                     RQENTIYDGRFQIPTFDEILQLCERLTEELGRVIGVYPETKHPTYFASIGKPLEPALV
                     ATLDRAGLNRKDAPVFVQSFETSNLRRLNDELHVPLVQLLEANGRPADLAAAGDPRTY
                     ADLATPAGLADIASYADAIGPDKNMVVARNADGALGAPTPLVGNAHAVNLLVHPYTFR
                     NENHFLPANLRSTGSAVDYGNVFAEYAAFFAAGVDGVFADNPDTAVAAREAQ"
     gene            complement(3491..4423)
                     /locus_tag="VFE39_05195"
     CDS             complement(3491..4423)
                     /locus_tag="VFE39_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6258030.1"
                     /translation="MRRAAPDPSSVRLPGPWTHRAVSANGIRLHVAECDGGGPLVVLL
                     HGFPEFWWSWRHQLSDLADQGFRAVAVDLRGYGDSDKPPRGYDLWTLAGDVAGLIRAL
                     GESRAHVVGHDWGGLIAWTVAALHPRLVRSLAVIAAPHPLAVRAAVLRDPRGQGRATL
                     GYALGFQVPRWPERSLRAEDGARVGRIMCAWAGPAWAASSDFADAVEYYRRAIQVPGV
                     VHCAMEYYRWALRSQFRAEGPRFAAAVSRPCAVPVLQVHGALDPCLLVSTAVASQRWI
                     GGDHRRQVLPATGHFPHQELPSETTALIAGFLSS"
     gene            complement(4429..4950)
                     /locus_tag="VFE39_05200"
     CDS             complement(4429..4950)
                     /locus_tag="VFE39_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019813808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET6258031.1"
                     /translation="MASPTSSSGADVPPVLPSIPLSREPVRSLEEQSIGSLVREATEQ
                     VSTLVRAEVELARAEMTAEVKKGLQGSIFFVVALTILLFSLFFAFFALGELLALWLNR
                     AASFAIVFGIMLVVAGLFGLLGFLRVRRIRKPERTISSLKESVEVLSHRGRGEPRSAA
                     PHELNGRPASSAR"
     gene            complement(5186..5614)
                     /gene="arfB"
                     /locus_tag="VFE39_05205"
     CDS             complement(5186..5614)
                     /gene="arfB"
                     /locus_tag="VFE39_05205"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HET6258032.1"
                     /translation="MADGDLPVRGSLVLPERELHWRFSRASGPGGQGVNTTDSRVELW
                     FDLARSPSVPEPLRERALTRLSARLVDGVLTVVASEQRSQLRNRQAARERLAEILRAA
                     TAADPPRRRPTRPSAGAKRRRLDAKTRRGAVKRLRGRPED"
     gene            5817..6557
                     /locus_tag="VFE39_05210"
     CDS             5817..6557
                     /locus_tag="VFE39_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6258033.1"
                     /translation="MALSETQNEPTGARRIARPVPLRESVYNAILDMIVSRNLQPGQH
                     LVESELAVMLGVSRQPVREALQWLKNDGWVDLRPGLGAYVHTPTVEEADQLLAVRRLL
                     ETESARLAAQHATEAGVEHLRELCRQGHAALKAEDVGTMVALNAELHATVMGLSCNDV
                     LVELASQVDRRVRWYYTPVARHRGAQSWTEHSALIDAIAAGDGDRAAEVMHAHTEHTR
                     LSYLEQQDLDKPGADAGAPARPVTARQP"
     gene            6869..7060
                     /locus_tag="VFE39_05215"
     CDS             6869..7060
                     /locus_tag="VFE39_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258034.1"
                     /translation="MRTLRKIVHSLSTMPRGFNADGRALGLGLAAALQAQRARGADVQ
                     PIQTSTRPTQAEGPYSRAS"
     gene            complement(7123..8514)
                     /locus_tag="VFE39_05220"
     CDS             complement(7123..8514)
                     /locus_tag="VFE39_05220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HET6258035.1"
                     /translation="MGEPDRSVAFQEVRDWYGRSYRVGEDPMDLIGRPRSSVLRQAWV
                     PMAAVGVLQYGYGAAVPALMGRNGWGLAGAFWLLAVWTVFQAGVGFPTAYLRERRRLG
                     PRTVMITGAALSALGLVSLAHTTSLFGALLGYSVLGGTGAGLVYAACTSTVAKWFPER
                     MAGRVSRVTGAFAYGSVPFIVAAVIGLDASNLALVFDVAAVVLFLLVAGTGMYFRDPP
                     ARWWPAEVDPRAWGLGQSGNPGRRKNPPAIREYSARQAMRTRVMPVMYAILIGAGAVS
                     LFNAAFVVVFATGFAAGAGVIALAAGVLAGVNGAGRAASVGISDRIGRCATLNIVLLV
                     QAIAQVLFALSASTGSTIAMVIAAALAGLGGGGFYPLFASIAREYFGEQRALEVHGLV
                     YSAKAGGGLLGVGLAALAVTSWGFAATFLVASFVSLGSAWATGALQRPGLPSTLPLGR
                     VPKSRPAPSRYAT"
     gene            8650..>9386
                     /locus_tag="VFE39_05225"
     CDS             8650..>9386
                     /locus_tag="VFE39_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET6258036.1"
                     /translation="MDLYEHQAKDLFDAHGVPVSRGTVAVTAEEAEAVARDIGGAVVV
                     KAQVKTGGRGKAGGVKLATTPDEAKEKAAAILGMDIKGHTVHRVLVAEASQIAQEYYV
                     SFLLDRSNRTFLAMASAEGGMEIEQLAAERPEALAKVPIDPLTGCDVGKARFIVDAAG
                     FPQEVAGAAAEVIAKLWATFVSEDATLVEVNPLISDTAGRVIALDGKVTLDENAGFRH
                     PDHAGLVDERTENPLEAKAKSKGLNYV"
BASE COUNT         1562 a         3379 c         3257 g         1188 t
ORIGIN      
        1 tgggcagcga cggcagcgac ggcagcgacg gcagcgagcc gacaaccggc agcgacggca
       61 gggagtccgt gctcggtagc gacggcaggt cgccgacaac cggcagcgac gacagcgacg
      121 ggagggcgcc gacgaccggc aaggacggca gcgacggcag ggcgtcggtg ctgggcagcg
      181 acggcagcga cggcagcgac ggcaggtcgc cgacaaccgg cagcgacgac agcgcatcgg
      241 tactgggcag cgacgggagc gcgtcggtgc tgggcagcga cggcagcgtg tccacagccg
      301 gcaggttcag ctgcagagcg ctgaggtccg gggcacccac gggcagctgg gggaggccgg
      361 cgcctccacc accgaggttg agcagcccga ggtcgatccc ctggaggtcc agcccgtcgg
      421 tgggcaggcc gttggtgggg aacgcggcga cgtccgtgcc ggggaagccg ggcagcgcct
      481 gggcgttgtc gaggccggcg tccatgctgc cggcggtctc gtcgtcggtg tagtgaccgc
      541 tcaggtcggc ctcggcggca ctggccgggc ctgcgatggc gagcagcgcc accggcgcgc
      601 ccaggacacc gagcgcggcc cggccggcaa tgcgcgacgt gcggctgcgg gtggcgtgtc
      661 tcggacgtgc gttcttcgtc atgcgagtcc tcggttctcg ggggagcgga cggatgaccc
      721 cggagcagac cctcgatctc ggccatcacg gggagcgacg acgacgtgga tctgaccagg
      781 caggcgtacc ctctactgcc acccgttcgg acgcaagtcg cagtaattga ggacagaacg
      841 gagatctact gacccattag ggcaatacgc tcgcccttta gggcggtgac acgtgggcac
      901 cgctcaacca acgtcacccg atcgagtgac aacctcggcg ccttgcggga tcgtcacctc
      961 gccccgcgaa gcgcgcaggg cgatgtcggt gcggtggtgc gaaccggcga gatgcacgcc
     1021 ctcgagccga cggtaggcgt cgtcgcgggc cagcgcgaga tccgcaccgg tgccgagcac
     1081 agaaaccacc cgcccactcg tggaaaccac agccccgtcg tcccggcggc gggtgccggc
     1141 gtgcagcacc ccgtcggcct ccgatccgct gatcacatcg cccaggcggg gcaggcccgg
     1201 gtagccctcc gcagccacga cgaccgtgac ggccgcgccg tcggaccact ccagcggagg
     1261 atgggccacc agcgcgcccg tggccgcgga gtgcagcagc cgtgccagcg gcgtgcgcag
     1321 cagcgccagc acgacctgtg tctccgggtc gccgaaccgg cagttgaact cgatcaccga
     1381 gggacccgcc gacgtcaggg cgagcccggc gtagagcaat ccttggaatg gggtgccgcg
     1441 gcgcaccatc tccgccgcga cgggcgccac gacggtcgcc acgagctcat cggccaaggc
     1501 gggtgacgcc cagggcagcg gtgcgtaggc gcccattccg ccggtgttcg gaccgcagtc
     1561 gtcgtcgccg acgcgcttga agtcctgagc cggcagcagc ggcaccaccg tctccccgtc
     1621 gacgaggcag aacagcgaga cctccgggcc gtccaggtac gactccagta gcacggggtg
     1681 cccgtcgtcc aacagcgtgc gggcgtgcgc gcgggccgcg tcgaggtcgg agctcaccac
     1741 cacgcccttg cctgccgcca acccgtcgtc cttcacgaca tagggcgggg cgaaacggga
     1801 gagcgctgcg tcgaggtgcg ccgggttgtc gacgatctcg gacgtggcgg taggcacagc
     1861 ggccgccgcc atgacgtcct tcgcgaagct cttggagccc tcgatgcggg cggcttctgc
     1921 actcggaccg aaacacgcga tccccgcacc acgcacggca tcggctacgc cggcgacgag
     1981 cggcccctcg ggcccgacga cgaccaggtc ggccttccac tccaccgcaa gttccgctgc
     2041 cgcaaccgga tccagcacgt cgagcccacg ctgctcggcc accgcggccg tgcccgcatt
     2101 gcccggcgcg caggcgagcg cggtgacttc cgggtcctgc gccatggcga ggacgatggc
     2161 gtgctcacga gcaccggacc ccacgacgag gactcgcacg aaacagaatc gtatcggtcg
     2221 cgacccgtgg ttccaccgca gatgccggtc acggttgcgg atcgcacatg ccgttcaccg
     2281 ggtggacccg caacagccac ctcccggtgg cggtgggctc cctcgatcgg ggcaaggtag
     2341 gcaacgtgtc gtccgcgttc accgcctccg ccctgaccac cgcccctacc ggcgcctccg
     2401 cggctgccgg aaaggcccgg gagaagtcag gagcgcatgt gctcgtgatc ggccaccgcg
     2461 gcgcatcggg ctaccgcccg gagcacacgc tcgcctccta cgagctcgca gccaggatgg
     2521 gtgccgactt cgtcgagccc gacctcgtca gcacctccga tggggtgctc gtcgcccgcc
     2581 acgagtcgga gatcggcggc accacggacg tcgcgaaccg cccgcagttc gccgaccgga
     2641 ggaccaccaa gacgatcgac ggcgtcctgc gcacgggctg gttcaccgag gacttcacgc
     2701 tcgcggagct caagacgctc cgggccaagg agcggatccc aggcttccgc caggagaaca
     2761 cgatctacga cggtcggttc cagatcccga cgttcgatga gatcctccag ctctgcgagc
     2821 ggctgaccga ggagctcggg cgcgtgatcg gcgtctatcc ggagacgaag caccccacct
     2881 acttcgcctc gatcggcaag ccgctggagc ccgcgctcgt cgccacgctg gaccgggcgg
     2941 ggctcaaccg gaaggacgca ccggtgttcg tgcagtcctt cgagacgtcg aacctgcgaa
     3001 ggctgaacga cgagctgcac gtgccgctgg tgcagctgct cgaggcgaac ggccgccccg
     3061 cggacctcgc ggcagcagga gacccccgca cctacgccga cctcgcgacg ccggcgggac
     3121 tcgccgacat cgcgtcgtac gccgacgcca tcggaccgga caagaacatg gtcgtggcgc
     3181 gcaacgccga tggcgcgctc ggcgcaccca ccccactcgt cggcaacgcg catgcggtga
     3241 acctgctggt gcacccgtac acgttccgca acgagaacca cttcctgccg gcgaacctgc
     3301 ggagcaccgg gtcggccgtc gactacggca acgtgttcgc cgagtacgcg gcgttcttcg
     3361 ccgcaggcgt cgacggcgtc ttcgctgaca acccggacac cgcagtagcc gcccgcgagg
     3421 cccagtaacc cgccccccgc ccacccgcga gtcgcccgtt tcccgcccgc gaggcgccct
     3481 ctttccgcct tcaggaggac aagaagccgg cgatcagggc agtcgtctct gacggcagct
     3541 cctggtgcgg gaagtggccg gtcgcgggca ggacctggcg ccggtggtcc ccgccgatcc
     3601 accgctgtga cgccaccgcc gtgctgacga gcaggcacgg gtccagcgcg ccgtggacct
     3661 gcagtaccgg aaccgcgcac ggccgggaca ccgcagcagc gaagcgcggc ccctcggcac
     3721 ggaactggga ccgcagggcc cagcggtaat actccatcgc acagtgcacg accccgggca
     3781 cctggatcgc ccggcggtag tactccaccg cgtccgcgaa gtcgctcgac gccgcccagg
     3841 ccggcccggc ccaggcgcac atgatccggc cgacgcgggc gccgtcttcg gcccgcagcg
     3901 agcgctccgg ccaacggggc acctggaagc cgagggcgta gcccagtgtc gcccgaccct
     3961 ggccccgggg gtcgcgcagc actgcagcgc gcaccgccag cggatgcgga gcagcgatca
     4021 cggccagcga ccgcacgagc cgcgggtgca gcgccgcgac agtccaggcg atgagcccac
     4081 cccagtcgtg tccgaccacg tgtgcgcgcg actcgcccag cgcgcggatg agtccggcga
     4141 cgtcaccggc cagggtccac aggtcgtacc cgcgcggggg cttgtctgag tcgccgtagc
     4201 cgcggaggtc gacggcgacg gcccggaagc cctggtcggc gaggtcgctg agctggtggc
     4261 gccacgacca ccagaactcc gggaacccgt gcagcagaac gaccagcgga ccacctccgt
     4321 cgcactcggc gacgtgcagg cggataccgt tcgccgatac cgcgcggtgc gtccagggac
     4381 cgggcaggcg caccgacgac ggatccggcg cggccctgcg caatccggtc agcgtgcgga
     4441 ggaggcgggc cggccgttga gctcgtgcgg cgccgcacta cggggttcgc cacgaccacg
     4501 gtgcgacagg acctccaccg actccttgag gctggaaatc gtgcgctccg gcttgcggat
     4561 ccgccgcacg cgcaggaagc cgagcagccc gaagagcccg gcgacgacca gcatgatccc
     4621 gaaaacgatc gcgaaggagg cggcccggtt gagccagagc gcgagcagct caccgagcgc
     4681 gaagaacgcg aagaacaggc tgaacagcag gatcgtcagc gccacgacga agaagatgct
     4741 gccctgaagg cccttcttca cctcggccgt catctcggcg cgggccagtt ccacctcggc
     4801 ccgtaccagc gtggatacct gctcggtggc ctcgcggacg agactgccga tcgactgctc
     4861 ctccagcgac ctaaccggct cgcgcgagag cgggatcgag ggcagcacgg gcggaacgtc
     4921 cgcgccgctg gagctggtcg ggctggccac cgcggtcctc ctgcacgtgc tgctcggctt
     4981 gtcggtgatc gggtggccat ccttccacgg tcacggggcc attgcccgat caggagcgta
     5041 ggcgcgccgc tgcctcaatc ggtcgggtcg aatccgtccc ggccgacgcc gcgggcatac
     5101 atgccccggg cgcaccagca tcgtcgacgc gcgcggcagg ggccgacagc gcgccgcgtt
     5161 gcgatccgaa ccggggagca caacgtcagt cctctggccg accgcgcagc cgcttgaccg
     5221 ccccgcgccg ggtcttcgcg tcgagacgcc gccgcttcgc ccccgccgag ggacgcgtgg
     5281 gcctgcgcct cggcgggtcc gccgccgttg ccgcccggag gatctctgcc agccgctcac
     5341 gggcggcctg ccggttgcgc agctggctgc gctgttcgga cgccaccacc gtcagcacac
     5401 cgtcgacgag ccgtgccgag agcctggtca gcgccctttc ccggagcggc tccggcaccg
     5461 acggagaccg ggcgaggtcg aaccacagct ccactcgcga gtccgtggtg ttcacaccct
     5521 gtccgccggg gccggacgca cgggagaacc gccagtgcag ctcccgttcg ggcagcacca
     5581 gcgatccgcg caccggcagg tcgccatcgg ccatccggtc agcatgccgc aggatccggg
     5641 ccggcgagac gccctcgtcc ccacatcgga cagatcgggc ggagtacgtg agcggcttca
     5701 ccgcccggta cctgcgacaa agtggcacgg cactttgtat gctgtgggca gtcgacaaga
     5761 caggagtgcc cccggaaggg agccggaccg acccaccgtc ccacctggag tccacgatgg
     5821 cactgtccga aacgcagaac gagcctaccg gcgcgcgccg aatcgcccgg ccggtcccgc
     5881 tccgcgagag cgtctacaac gcgattctgg acatgatcgt cagtcggaac ctccagccgg
     5941 ggcagcacct ggtggagagc gagctcgcag tgatgctggg cgtctcccgc cagccggtgc
     6001 gtgaggcgct ccaatggctc aagaacgacg gttgggtgga tctccggccc gggctcgggg
     6061 cgtacgtcca cacgcccacg gtcgaggagg ccgaccagct gctcgcggta cgcaggctgc
     6121 tggagacgga gtcggcacgg ctggccgcac agcacgccac cgaggccggg gtcgagcacc
     6181 tgcgggagct gtgccgccag ggccacgcgg ctttgaaggc cgaggacgtg ggcaccatgg
     6241 tcgccctcaa cgccgagctc catgccaccg tgatggggct ctcctgcaac gacgtactgg
     6301 tcgagctcgc gagccaggtc gaccggaggg tgcgctggta ctacacgccg gtggcgcggc
     6361 accgtggcgc ccagagctgg acggagcact cggccctgat cgacgcgatc gccgcaggcg
     6421 atggggatcg cgccgccgag gtgatgcacg cgcacaccga gcacacgcgc ctctcgtacc
     6481 tcgagcagca ggacctcgac aagcccggcg ctgacgccgg ggcgccagcg cgtccggtca
     6541 cggcccgcca gccctagacc gaaccgcgca ccaccctttc ccgtcgttgt cgacgggccg
     6601 tcggtcgacg acgactgctg tatcaccttc cccgtcgttg tcgacgggcc ctcggtcgac
     6661 gatgactgct gtgtcatcgg cgtcgacgcc gagcttcgcc acgccccccg ctcctgatcg
     6721 agccacgggg ataccccatc acgtgcatcg gtgcgcgacg cgcattgctg cgcggtacct
     6781 gcttccacct cgccgattcg acggcgcaac tgcggccgcg cattaccgga ccgcagaacg
     6841 ggaaagacct atcacagagg tgatgagaat gcgaacgctc cgcaagattg tgcactccct
     6901 gtccacgatg ccgcgcggct tcaacgccga cggccgggca ctagggctcg ggctcgccgc
     6961 cgcactgcag gcccagcggg cccgcggggc agacgtccag ccgatccaga cctcgacgag
     7021 gccgacgcag gcggagggcc cttactcgcg cgcctcctga tcgcaggcct gggttccatc
     7081 cctgggcggc tccccgcacg gagctgccca gggatggtgg cgtcaggtgg cgtagcgcga
     7141 aggagctggt cggctcttcg gtacccgccc caagggcagg gtgctcggca ggcccgggcg
     7201 ctgcagcgcc cccgtggccc atgccgatcc gagcgatacg aacgacgcga cgaggaacgt
     7261 ggcggcaaag ccccaggacg tcacggcgag tgcggccaga cccactccga gcaggcctcc
     7321 cccggccttt gcactgtaga cgagcccgtg cacctcaagg gcgcgctgct ccccgaagta
     7381 ctccctcgca atgctggcga acagcgggta aaagccgccg ccgcccaggc cggccagtgc
     7441 cgcagcgatg accatcgcga tcgtcgaacc cgtgcttgcg gacagcgcaa agagcacctg
     7501 cgcgatcgcc tgcaccagca gcacgatgtt cagcgtcgcg cagcggccga tgcggtcgga
     7561 aatgccgacg gacgccgccc gtccggcacc gttcaccccg gcgagtacgc cggcggcgag
     7621 tgcgatcacg cccgcgcctg cggcgaagcc cgtggcgaag accaccacga acgcggcgtt
     7681 gaacaacgag acggccccgg cgccgatcag gatcgcgtac atgaccggca tgacccgggt
     7741 gcgcattgcc tgccgggccg agtactcgcg gatcgccgga gggttcttcc gccgcccggg
     7801 gttcccggac tgcccgagac cccatgcccg cggatcgacc tcggcgggcc accaccgagc
     7861 cggcgggtcg cggaagtaca tcccggtgcc cgccacgagc aggaagagca cgacggcggc
     7921 cacgtcgaag accagggcga ggttggaggc gtcgagcccg atgactgcgg cgacgatgaa
     7981 gggcacggag ccgtacgcga acgccccggt caccctgctg acccgcccgg ccatgcgttc
     8041 ggggaaccac ttcgccacgg tggaggtgca cgccgcgtag acaagcccgg cgccggtgcc
     8101 tcccagcacg gagtagccca gcaacgcacc gaacaggctc gtggtgtgcg ccagggagac
     8161 caggcccagc gcgctgagcg cggcgcctgt gatcatgacg gtgcggggcc cgaggcgacg
     8221 gcgctcgcgg aggtaggcgg tcgggaagcc cactcctgcc tggaagacgg tccacaccgc
     8281 gagcagccag aaggcgccgg caagacccca cccgttgcgc cccatgaggg cgggcaccgc
     8341 agctccgtag ccgtactgca gcacacccac ggctgccatc gggacccacg cctgtcggag
     8401 caccgatgat cgcggacgcc cgatgaggtc catcgggtcc tccccgacgc ggtagctacg
     8461 gccataccag tcgcggacct cctggaaagc aacactgcga tccggctcac ccatgacgac
     8521 tccctccata ctgtatgcac gatcatgcga tgttgtgagc tgcggcacaa gtggtctgga
     8581 caaattcgtt ccatactgca tacagtcgac cagctcttga cgttgtcgtc agacaggaga
     8641 tgccgcagcg tggacctcta cgagcaccag gcgaaggact tgttcgatgc ccacggcgtg
     8701 ccggtgtccc gcgggacggt cgcggtcacg gccgaggagg ccgaggccgt ggcccgcgac
     8761 atcgggggcg ccgtcgtggt caaggcgcag gtgaagacgg gcggccgggg caaggcgggt
     8821 ggcgtcaagc tcgcgacgac gccggacgag gccaaggaga aggccgcggc gatcctgggc
     8881 atggacatca agggccacac ggtgcaccgc gtgctggtgg ccgaggcctc acagatcgcg
     8941 caggaatatt acgtctcgtt cctgctcgac cggtccaacc gcactttcct ggccatggcg
     9001 tcggccgagg gcggcatgga gatcgagcag ctcgccgccg agcggccgga ggcgctcgcg
     9061 aaggtgccga tcgatcccct gaccggatgc gacgtcggca aggcccgctt catcgtcgac
     9121 gccgccgggt tcccccagga ggtcgccggc gctgccgccg aggtcatcgc gaagctgtgg
     9181 gccacgttcg tctccgagga cgcgaccctc gtcgaggtga acccgctgat ctccgacacc
     9241 gccggcaggg tcatcgcgtt ggacgggaag gtgacgctcg acgagaacgc cggtttccgg
     9301 catcccgacc atgctgggct ggtcgatgag cgtacggaga acccgttgga ggcgaaggcg
     9361 aagtcgaagg gtctcaacta cgtcaa
//
LOCUS       DASRSC010000110         7284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4292468,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000110 DASRSC010000000
VERSION     DASRSC010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4292468"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1900)
                     /locus_tag="VFE39_05230"
     CDS             complement(221..1900)
                     /locus_tag="VFE39_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258037.1"
                     /translation="MRLVFRSDDHEEYSAVRDRLRLHIVAWARRRGIDVEPSLVAAAL
                     DHKHGVDGRLGHWTRAHVADALAVWFPRTVPLLEDDRDAVPTALHALIGFLADHDWLD
                     SRSAPPAELHAQVTDSTPALHDALDDERNHDLGTFWAVQMLRHGVATADPAAVGRFLQ
                     QVHAGELEIDRAALEEIQRRQEAESAEPPRPELPPVLLPGAAQMLAAADSSVALARLR
                     AFTRWVRAGRAVTREGRLLLSDARAVADALDLDHFSRDRARTSDDLPEISLLLVWARQ
                     ARLVRVVNGRLVQVKSAAPLLSRPIELWRRAFEAVGRIGEHFGGSNVFGAPSLFGMSL
                     GEAFPMLLLQLYSAGGDAIPVELFHRVVRDTVNDRMGCVVDDLAGDIEHRLWRRDVTA
                     LLDALELLGAVHLSESLDGENHDELIRLAGRDDPDPTLVSLTPLGVWAVQEMLTEQGV
                     HAPLVGELADEDIEYVCVRCTGLRPEIVEAELTAWVGARTGRTAAQEIMRYLQRVEDR
                     AYRDLALFALSRAGAPGREAARWRGRPAVALRAPATGAQPRVECDARARRP"
     gene            2088..3350
                     /locus_tag="VFE39_05235"
     CDS             2088..3350
                     /locus_tag="VFE39_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6258038.1"
                     /translation="MSGPTVRIVMNGVTGRMGYRQHLIRSVLAIRDDGGVLRPDGTRL
                     QIEPVLVGRSRDKLADIARRHDIELFHTDLDAALAEDGTSVYFDSQVTSERKKAILRA
                     IAAGKHVYTEKPLAESVAEGMELVEAARTAGIVDGVVHDKIYLPGLIKLKRLIDAEFF
                     GRIISVRGEFGYWVFEGDLLPAQRPSWNYRAEDGGGITLDMYCHWNYVLEHLFGGVEA
                     VMTRAVTHIPQRWDEQGNPYEATADDAAYGILELAGGVIAQINSSWAVRVDRKELVEF
                     QVDGTHGSAVAGLFGCRIQPRVCTPKPVWNPDVPTDQDFRAQWEEVPDHEEFTNGFRA
                     QWERFLLDVDAGRPHPYDFMSGVRGLRLVDAALRSSAEGVRVVLPTVSEPPVAAPETA
                     EGPVGHPEARVAQPEARVAQPEAPGASA"
     gene            3347..4528
                     /locus_tag="VFE39_05240"
     CDS             3347..4528
                     /locus_tag="VFE39_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET6258039.1"
                     /translation="MTQVVARTGHRIDVPVAGGGWRAVELAEPREWAEHPGPYRSRVA
                     FAAAHVAADPWGENVPGAPAVVDWDATLAFRRHLFSHGLGVAEAMDTAQRNMGLDWVA
                     VQELVSRSAEQARAHGARIAAGAGTDHRPDIRSVDDVLIAYGEQVEFVEGTGAQVILM
                     ASRQLAAVAAGPEDYLRVYDELLRQVREPVILHWLGEAFDPQLRGYWGSPDVSAATAT
                     FLQLVAAHAAKVDGVKVSLLSAEHEIGLRAALPPGVRLYTGDDFNYPELIRGDGTHHS
                     DALLGAFSAIAPAASAALAALDDDDLVRYDAEFAPTLALSRHVFTAPTFYYKTGIAFL
                     AWLCGHQPGFTMVGGLQSARSVVHLGEAFALANEARLLPDPERAAEKFRGLLAVAGAA
                     R"
     gene            4597..5421
                     /locus_tag="VFE39_05245"
     CDS             4597..5421
                     /locus_tag="VFE39_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6258040.1"
                     /translation="MARLSLNQRTTAHWTLREAIDGAVAAGLPAIGLWREPVAEVGVD
                     AARRWVDDAGLRVSSLCRGGFFTASDPVARATAHEDNLRAIDEAAALGTRALVLVPGG
                     LPAGDKDLTGARARAVEAIAALVPHAVERGVTLGIEPMNPIYAADRGVVSTLAQALDI
                     AGAFAPHEVGVVVDTFHLWWEPDVHAQIARAGERIVSYQVCDWITPLPADTLLARGMM
                     GDGHVDFAAFTRAIAAAGYPGDVEVEIFHADVWAAPGEQVIATLARRYVELVQPYL"
     gene            complement(5569..6186)
                     /locus_tag="VFE39_05250"
     CDS             complement(5569..6186)
                     /locus_tag="VFE39_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6258041.1"
                     /translation="MSRSPWADTGLFLREFVRDPLHTAALAPSSPALAAAMTASLPGT
                     GAPVVVELGPGTGAFTRVIQERTGGRARHIAVELNPGWAQLLAQRYPQVEVVLADARE
                     LPALLADRGIAAVDAIVSGLPWVAYSPGPHARGLHAVITEALAARGVFTQFAYTWTRW
                     SPPARRQLADLRAHFADVVISRPVWRNLPPAVVYEARQPQRRRGD"
     gene            6257..6583
                     /locus_tag="VFE39_05255"
     CDS             6257..6583
                     /locus_tag="VFE39_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258042.1"
                     /translation="MRENPVHPHRLQQLVASVPESITAEQRARLLAHVQTSDGCRARA
                     DRVRHELRRVLDGAEGGADRAVDLACELDSLERVQERVDGWLGELVDELTHTPRVVHY
                     DDGVPA"
     gene            6644..>7284
                     /locus_tag="VFE39_05260"
     CDS             6644..>7284
                     /locus_tag="VFE39_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6258043.1"
                     /translation="MDLLELTPRLHLLRFSVGQAYLWRDGNDLTLVDTGPAGSGPAIA
                     DAVQRFGTLRRVVLTHFHDDHAGSAAEVASWPGVTVLAHRDDAPIIRGERQGPPPDFT
                     EFERELHSRVAHGLPPAPPARVDVELTGGERLDIGEGARVVHVPGHTDGSIGLHLAAD
                     RVLFTGDVVAEHGGELMLGPFNLDRERAAVSLRVLAGIDADIACFGHGDAMVGG"
BASE COUNT          982 a         2612 c         2683 g         1007 t
ORIGIN      
        1 cccgtggagg ggtgaggggg tgccccggtg gtgcctgccg ctgcgccggg acgatgaacc
       61 actgcttggg cgctccggac cagctccagt aggcgtcgga attcaccggc tgctcttctt
      121 ccgtggggga cgggcgacga ctgtcacccg ccccctactc gcacagcacg accccgacgt
      181 gacgggtggt cccgcgtgtc actcgaacgg tcgtgtggcg tcagggcctg cgtgctcggg
      241 cgtcgcactc gacgcgcggc tgggcccccg tggcgggtgc gcgcagggcg acggccgggc
      301 gcccccgcca gcgcgccgcc tcgcggccgg gggcaccggc gcgggacagg gcgaacaagg
      361 ccaggtcccg gtatgcgcga tcctcgaccc gctgcaggta ccgcatgatc tcctgggctg
      421 ccgtccgccc ggtgcgggca cccacccagg cggtcagctc ggcttcgacg atctcggggc
      481 gcaggccggt gcatcgcacg cagacgtact cgatgtcctc gtccgcgagt tccccgacca
      541 gtggcgcgtg caccccttgc tcggtgagca tctcctgcac ggcccagacc ccgagcggtg
      601 tgagcgagac cagcgtgggg tcggggtcgt cgcggccggc gagccggatc agttcgtcgt
      661 ggttctcgcc gtcgaggctc tcggacaggt ggacggcccc gaggagctcc agggcgtcga
      721 gcagcgccgt gacgtcgcga cgccacagcc ggtgctcgat gtccccggcg aggtcgtcga
      781 cgacgcagcc catccggtcg ttgacggtgt cgcgcacgac gcgatggaac agctcaacgg
      841 ggatcgcgtc tccgcccgcc gagtacagct gcaacagcag catcgggaac gcctctccga
      901 ggctcatccc gaacagcgat ggcgccccga acacgttgct gccgccgaag tgctcgccga
      961 ttctgccaac ggcctcgaac gcgcgccgcc acagctcgat cggtctgctc agcagcgggg
     1021 cagcgctctt cacctgcacc agccgtccgt tcacgacgcg gacgagccgg gcctgccgcg
     1081 cccagaccag cagtagcgat atctccggaa gatcgtcgct ggtgcgggcc cggtcgcggc
     1141 tgaagtggtc gaggtcgagt gcgtcggcca ccgcacgggc gtcggagagc aggagcctcc
     1201 cctcccgcgt caccgctcgc cccgcccgta cccaccgggt gaacgcgcgc agccgggcga
     1261 gcgcgacgct ggagtcggcg gcggccagca tctgggccgc gccgggcagc agcacgggcg
     1321 gcagctcggg ccgcggcggc tccgcgctct cagcctcctg ccggcgctgg atctcctcga
     1381 gcgccgcccg gtcgatctcc agctcaccgg cgtgcacctg ctggagaaac cggccgacgg
     1441 cggccgggtc cgccgtcgcg acgccgtggc gcagcatctg caccgcccag aaggtgccga
     1501 ggtcgtggtt gcgctcgtcg tcgagggcgt cgtgcagggc cggcgtggag tcggtgacct
     1561 gggcgtgcaa ctccgcgggc ggcgccgagc gcgagtcgag ccagtcgtgg tcggcgagaa
     1621 atccgatcag ggcgtgcagc gcggtgggca cggcgtcgcg gtcgtcctcg agcagcggca
     1681 ccgtgcgcgg gaaccacacg gccagcgcgt cggccacgtg cgcgcgggtc cagtggccga
     1741 ggcgcccatc gacgccgtgc ttgtgatcga gtgccgcggc gaccagtgac ggttccacgt
     1801 cgatgccgcg ccggcgggcc cacgcgacga tgtgtagccg caggcggtcg cggacagcgg
     1861 agtactcctc atggtcgtcg gagcggaaga cgagacgcac tcggcccaca ttggcagccc
     1921 cacctgaacg gcctacaggc ggggccgctc aatcactctg cgtagtagtc acggcgtcgg
     1981 ccttgccgct gcgtcaccgt ccgtcctatc ctccggaaag cgcttacttc tcgtgcttac
     2041 cacgctggac ggtccgcagc cgaacccgga gagagcaggt agagcccatg tcaggaccca
     2101 ccgttcgcat cgtgatgaac ggcgtgaccg ggcggatggg gtatcgccag cacctcatcc
     2161 ggtccgtact cgcgatccgc gacgatggcg gagtcctacg tcccgacggc acccgcctcc
     2221 agatcgagcc ggtgctggtg gggcgcagcc gggacaagct ggccgacatc gcccgccgcc
     2281 acgacatcga gctgttccac accgacctgg acgccgcact cgccgaggac gggacgtccg
     2341 tttacttcga ctcgcaggtc acgagtgagc gcaagaaggc gatcctgcgg gcgatcgccg
     2401 ccgggaagca cgtgtacacg gagaagccgc tcgccgagtc ggtcgccgag gggatggagc
     2461 tcgtcgaggc cgcccggacc gccgggatcg tcgacggggt ggtacacgac aagatctacc
     2521 tgcccggcct gatcaagctg aagcggctga tcgacgccga gttcttcggc cgcatcatct
     2581 cggtgcgcgg ggagttcggc tactgggtct tcgagggcga cctgctgccg gcgcagcgcc
     2641 cgagctggaa ctaccgcgcc gaggacggcg gcgggatcac gctggacatg tactgccact
     2701 ggaactacgt gctcgagcac cttttcgggg gcgtcgaggc cgtgatgacc agagcggtca
     2761 cgcacatccc gcagcgctgg gacgagcagg gcaatcccta cgaggccacc gccgacgatg
     2821 ccgcctacgg gatcctcgag ctggccgggg gcgtcatcgc ccagatcaac tcgtcctggg
     2881 cggtgcgggt ggaccgcaag gagctcgtcg agttccaggt ggacggcacg cacggctcgg
     2941 ccgttgcggg cctcttcgga tgccgcatcc agccgcgggt gtgcacgccc aagccggtgt
     3001 ggaaccccga cgtgcccacc gaccaggact tccgcgcgca gtgggaggag gtgcccgacc
     3061 acgaggagtt caccaacggc ttccgcgcgc agtgggagcg cttccttctc gatgtcgacg
     3121 cgggccggcc gcacccttac gacttcatga gcggggtgcg tggcctgcgg ctcgtggacg
     3181 cggcgctccg gtcctcggcc gagggcgtgc gcgtggtgct gccgacggtc tcggagccac
     3241 ccgtggcagc gccggagaca gcggaagggc ccgtgggaca cccggaggcc cgggtggcgc
     3301 agccggaggc ccgggtggcg cagccggagg ctccgggggc gtcggcatga cgcaggtcgt
     3361 cgcgcgcacc gggcaccgga tcgacgtccc cgtcgccggg ggtgggtggc gcgccgtgga
     3421 gctcgccgag ccccgcgagt gggcggagca tcccggtccg taccgcagtc gcgttgcgtt
     3481 cgccgcggcc cacgtcgccg ccgacccgtg gggcgagaac gtgcccggtg cgcctgccgt
     3541 cgtcgactgg gacgcgacgc tcgcgttccg ccgccatctg ttctcccacg ggctgggcgt
     3601 cgccgaggcg atggacactg cgcaacgcaa catggggctc gactgggtcg cagtgcagga
     3661 gctggtgagc cgcagcgccg agcaggccag ggcgcacggc gcccggatcg ccgccggggc
     3721 cggcaccgac caccgccccg acatccggtc ggtcgacgac gtcctcattg cctacggcga
     3781 gcaggtggag ttcgtcgagg gcaccggcgc gcaggtgatc ctgatggcgt cgcgacagct
     3841 ggccgcggtc gcggccgggc cggaggacta cctgcgcgtc tacgacgagc tgctgcgcca
     3901 agtccgcgag ccggtgatcc tgcactggct cggcgaggcg ttcgacccgc agctgcgcgg
     3961 ctactggggc tcccccgacg tgtccgcagc gaccgcaacg ttcctgcagc tcgtcgcggc
     4021 gcacgccgcg aaggtcgatg gggtgaaggt gtcgctgctg tcggccgagc acgagatcgg
     4081 gctgcgggcc gcgctcccgc caggtgtgcg gctctatacc ggcgacgact tcaactatcc
     4141 cgagctgatc cgcggcgacg gtacccacca ttcggacgcg ctgctggggg cgttctccgc
     4201 cattgccccg gccgcgtcgg cggccctcgc cgcgctcgac gacgacgacc tcgtccgtta
     4261 cgacgccgag ttcgcgccca cgctcgcgct gtctcggcac gtgttcaccg ctccgacctt
     4321 ctactacaag accggcatcg ccttccttgc ctggctctgc gggcaccagc ccgggttcac
     4381 gatggtgggc ggtctgcagt cggcgcggtc ggtcgtgcac ctgggagagg ccttcgcgct
     4441 ggccaacgag gcccggctgc tacccgaccc ggaacgggcg gcggagaagt tccgggggct
     4501 cctcgcggtg gcgggggcgg cccggtgagt gccgtcctcg acatccccag cggcgccccc
     4561 gcccgcgtcg gcacgccggt gccgggcgac ccgcggctgg cgcggctctc cctcaaccag
     4621 cgcaccacgg cgcactggac cctccgcgag gcgatcgacg gagccgtagc ggccggcctt
     4681 cccgcgatcg ggctctggcg ggaaccggtg gccgaggtcg gcgtcgacgc tgcccgccgc
     4741 tgggtggacg atgcgggcct gcgggtgtcg tcgctgtgcc gcggcgggtt cttcaccgcg
     4801 tccgacccgg tggcgcgcgc caccgcccac gaggacaacc tgcgcgccat cgacgaggcc
     4861 gcggcgctgg gcacccgcgc gctggtcctc gtccccggcg ggctgccggc gggcgacaag
     4921 gacctcaccg gggccagggc gcgggcggtc gaggcgatcg cggcgctggt gccgcacgcg
     4981 gtcgagcgcg gggtcaccct cggcatcgag ccgatgaacc cgatctacgc cgcggaccgc
     5041 ggagtcgtct ccacgctggc tcaggcgctc gacatcgccg gggcgttcgc cccgcacgag
     5101 gtgggcgtcg tcgtcgacac cttccacctg tggtgggagc cggacgtgca cgcccagatc
     5161 gcgcgggcgg gcgagcggat cgtcagctac caggtctgcg actggatcac gccgctgccc
     5221 gccgacacgc tgctcgcccg cgggatgatg ggtgacggcc acgtcgactt cgccgccttc
     5281 acccgtgcga tcgccgcagc gggctatccg ggcgacgtcg aggtggagat cttccacgcc
     5341 gacgtctggg cggcgccggg ggagcaggtg atcgccacgc tcgcgcgccg ctacgtcgag
     5401 ctggtccagc cgtacctctg agtatcggga gctacgaaga tcgccgcggc ccgcaccagc
     5461 gcccccgcaa cggcgctggt gtgcgtctag gtgcgatcac gggcggtgat cgggtatgac
     5521 tcgcgcacca gggccctccc cagggcgctg agctacgtct ggctgcgatc agtcgccccg
     5581 gcggcgctgc ggttgccgag cctcgtagac gacggcgggc ggtaggttcc gccacaccgg
     5641 ccggctgatc acgacgtcgg cgaagtgcgc gcgcaggtcg gcgagctggc gccgggccgg
     5701 cggcgaccag cgtgtccagg tgtaggcgaa ctgcgtgaag acgccgcgcg cggcgagcgc
     5761 ttcagtgatc accgcgtgca ggccgcgcgc gtggggaccg ggggagtagg ccacccacgg
     5821 caacccgctg acgatcgcgt cgacggcggc gatgccgcgg tcggcgagca gtgccggcag
     5881 ctcgcgcgcg tcggcgagca ccacctcgac ctgggggtac cgctgtgcga gcagctgcgc
     5941 ccagcccggg ttgagctcaa cggcgatgtg ccgggcccgg ccgccggtgc gctcctggat
     6001 cacgcgggtg aacgccccgg tgccgggtcc gagctccacc accacgggtg cgccggtgcc
     6061 gggaagcgat gccgtcatcg cggcggcgag cgcgggcgag ctgggcgcca atgccgccgt
     6121 gtgcaggggg tcgcgcacga actcgcgcag aaacaggccg gtgtcagccc atggcgaccg
     6181 ggacatgatc ggaccgtagc ggccggggta gccgtcaccc gcccgggcgc cgtacggtcg
     6241 tggtgatgac cgtcacgtga gggagaaccc cgtgcaccca caccgcttgc agcagctcgt
     6301 cgcgtccgtt cccgagagca tcacggccga gcagcgtgcc cggttgctcg cccacgtcca
     6361 gacctccgac ggttgccgcg cgcgtgccga ccgggtgcga catgagctgc gtcgcgtgct
     6421 cgacggcgca gagggcggcg cggaccgcgc ggtcgacctg gcctgcgagc tcgacagcct
     6481 cgagcgggtc caggagcggg tggacggttg gctcggcgag ctcgtcgacg agctgacgca
     6541 caccccgcgg gtcgtgcact acgacgacgg cgtgccggcc tgaaccgctc ctcgttgcgc
     6601 gaacggcagg gccggccggc cctcggctcc ctacggtcgc aacatggatc ttctggagct
     6661 cacgcctcgc ctgcacctgt tgcggttctc tgtggggcag gcctacctgt ggcgcgacgg
     6721 gaacgacctg acgctggtcg acaccgggcc ggcaggctcc gggcccgcga tcgccgacgc
     6781 ggtgcagcgc ttcggaacgc tgcggcgggt ggtgctcacc cacttccacg acgaccacgc
     6841 cggttcggcc gcggaggtcg cttcctggcc cggcgtcacg gtgctcgccc atcgcgacga
     6901 cgcaccgatc atccgcggcg agcgccaagg cccgccaccg gacttcaccg agttcgagcg
     6961 cgagctgcac tcccgcgtcg cgcacgggtt gccgcccgcc ccgcccgcgc gcgtcgacgt
     7021 cgagctgacg ggcggcgagc ggctcgatat cggcgagggc gcccgggtgg tccacgtgcc
     7081 cggccacacg gacggcagca tcggcctgca cctcgccgcc gacagggtgc tgttcaccgg
     7141 tgacgtggtc gccgagcacg gcggcgagct gatgctcggc ccgttcaacc tcgaccgcga
     7201 gcgggcggcc gtctccctgc gtgtgctcgc cggcatcgac gcggacatcg cctgcttcgg
     7261 gcacggggat gcgatggtcg gcgg
//
LOCUS       DASRSC010000111         7966 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1345504,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000111 DASRSC010000000
VERSION     DASRSC010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7966
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1345504"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VFE39_05265"
     CDS             complement(<1..648)
                     /locus_tag="VFE39_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951469.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HET6258044.1"
                     /translation="MPDTRKAAVIGGNRIPFARANGAYARASNLDMLTATLDGLTARF
                     GLAGERLGEVAGGAVLKHSRDRDLTREAVLGSALSPYTPAYEVQQACGTGLETAILVA
                     NTIALGQIESGIAAGVDTASDAPVSLNDDLRRVLMELNGARSLGARLKALRGLRPGQI
                     VPEIPRNAEPRTGLSMGEHAALTALEWHVGREEQDELAAASHRNLAAAYDRGFFDD"
     gene            732..1385
                     /locus_tag="VFE39_05270"
     CDS             732..1385
                     /locus_tag="VFE39_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6258045.1"
                     /translation="MKVVRDKRTSRWDAHRRTRRAELTDAAIAAIRAHGAGVGMDEVA
                     ASAGTSKSVVYRHFTDRTQLYVAVCARVAEVLVAQVRAAMNDADGPRAKTAAGIAAYL
                     RLIEHDPEVYRFVVHRPLVDRVLDADPVADLVSLIGDEAAAVIAAQLRHTGSDTSAAV
                     PWGHGIVGLVRAAADNWLARPAGITREVLAAQLTDLAWSGLSGVVAPRPAIPKEELA"
     gene            1382..3346
                     /locus_tag="VFE39_05275"
     CDS             1382..3346
                     /locus_tag="VFE39_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_function: GO:0003997 - acyl-CoA oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HET6258046.1"
                     /translation="MTVDHSELQRVLDGRWADVRAEVRAQLGALALNPDPDLSTEEYR
                     ALTAENLRRLAESGRPHQGFDPAYGGGGDVGGVVTAFAALGYGDLSLLVKAGVQWGLF
                     GGAVQVLGTSRHHDKYLRSIMDGDLLGCFAMTETGHGSDVQHLRTTATYDPDNHEFEI
                     HTPDPGARKDYIGNAARDGRMAVVFAQLITGGTSHGVHALLVPIRADDDTTMPGVTIG
                     DCGRKAGLNGVDNGRLSFDHVRVPRENLLNHFADVAEDGRYSSSIESSSRRFFTMLGT
                     LVRGRISVAGGAGTATQKALALAIRYGATRRQFADPGTGEEVVVLDYLVHQRKLLPAL
                     ATTYALHFAQADLVSDMHDLQSFDAEQGSDSDTERRQRDLETRAAGIKALATWHATTT
                     IQTCREACGGAGYLAENLLPQLKADTDVFTTFEGDNTVLLQLVAKTLLSDYGRRVGKL
                     DLMGKARFAAGLVAGSVAERTGLRAGLRRGDVRDRAYQRWLLVQREEHLLAAAARRMR
                     RALAPGADQFAIFNGAQDHLLVAAQAHVDRIVADSFAAAVDRVDDTAVRALLDQVRGL
                     HALSVIEGDRAFFVEHGYLTPGRAKAVTHAVSTLCGQVRPNARTLVDGFGIPEAWLAC
                     PLLDGERDATAAATDPAPSTEEPTDLAAVG"
     gene            complement(3560..4339)
                     /gene="map"
                     /locus_tag="VFE39_05280"
     CDS             complement(3560..4339)
                     /gene="map"
                     /locus_tag="VFE39_05280"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET6258047.1"
                     /translation="MIELKTPAELDAVEAAGKIVADVLSAVREHAAPGVTPRQLDALA
                     ADLIAGTGARPSFLGYHPSFAPTPYPAVICASVNDAVVHGIPGRGRLRDGDLLSVDLA
                     VHLDGWCADAAFSTVVGQADPLDVALIETTERALAAGIAAAVSGARMGDVAHAIGVVG
                     RAGGYGLLAGHGGHGVGRAMHEAPHVPNEGRAGRGFTLRPGLVIAIEPMLLAGGADDY
                     SPDPDGWTLRTADGSRAAHAEHTIAITDDGPRLLTAGRPVN"
     gene            4373..4642
                     /locus_tag="VFE39_05285"
     CDS             4373..4642
                     /locus_tag="VFE39_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6258048.1"
                     /translation="MVRVPLTPADRERGERLGRLLRESRADRSLADVAGAAGISAETL
                     RKIETGRIPTPAFFTVSALAGALGLSLDTLVTATAPPLPEDRAAV"
     gene            complement(4691..4891)
                     /locus_tag="VFE39_05290"
     CDS             complement(4691..4891)
                     /locus_tag="VFE39_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258049.1"
                     /translation="MSDISQLRSGQFYVASEGLAFEKVQEPMCLSHHPASALTLEEVI
                     ARASAHTGGGAVAGCERGHWAD"
     gene            complement(5002..5910)
                     /locus_tag="VFE39_05295"
     CDS             complement(5002..5910)
                     /locus_tag="VFE39_05295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HET6258050.1"
                     /translation="MRAYGVTAFGGPENEAMLDLSVPEPGPGELLVRVRAAGVNPGDW
                     KLRDGGYGDVALPAVLGREVAGSVVATGPGVDGFAVGDEVFGGTPGMVGGWAEFALVT
                     ASFAAHRPERVAPADAATLPVAPATAYDALTGLDLAPGSTLLVNGAGGGVGVATLQLA
                     RARGLVVVGTASQGKHDLVASYGATPVVYGDGVAERIRAAAPQGVDAVFDTVGGDALR
                     AVAEVLPDRSRLVTIADRPLAARLGGSGVQRDRSTAVLITLAQLVAEGELDPLVTEIR
                     PLDEAGAALATVEAGHVRGKVAIEVA"
     gene            complement(5907..7067)
                     /locus_tag="VFE39_05300"
     CDS             complement(5907..7067)
                     /locus_tag="VFE39_05300"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET6258051.1"
                     /translation="MLVERMRPHTSTIFAEMSALALRAGAINLGQGFPDTDGPASLLS
                     DAAANIQAGVNQYPPGIGVPELRSAVAEHQRRFYGLDVDPDTVLVTTGATEAIASAVL
                     ALCEPGDEVVTFQPYYDSYAATIALAGAQLRAVALRPPSFGFDPDELRAAFSERTRIV
                     LVNTPHNPTGAVFDREQLTLIGELAAEHGAVIVTDEVYEHMIYDGREHIPMATLPGMA
                     ERTLTISSAGKTFSVTGWKVGWVHGPGELVSAVRAAKQFLTYVSGAPFQPAIAAALAL
                     PDSFYTELAGDLQRKRDLLSDGLRESGFTVFDTAGTYFVVTDVTALGFTDGAEFCWSL
                     PERVGVAAVPVSVFCADPDLGRSLVRFAFCKRDEVLTEAVARLAALRASVRA"
     gene            complement(7167..>7966)
                     /locus_tag="VFE39_05305"
     CDS             complement(7167..>7966)
                     /locus_tag="VFE39_05305"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET6258052.1"
                     /translation="CEAMVAAGLGEDLLLANEVLDARRLGTLVRDGARITVAVDSPET
                     IAAAAAGGVREVLIDVNVGLPRCGCPPERAGDLAGEARRGGLEVRGVMGYEGHLMLVA
                     DPADRARMTGEAMALLVAAAAEVGGELISAGGTGTYADNTWATEIQAGSYALMDTAYG
                     AVGQPFGQALSVLGTIVSVAPTHAVVDVGLKSLGMDHGLPAIPGAQVWFCSDEHTSFA
                     PEEPVRVGDRVRVLPAHVDPTVVLHEQMHVVDGDRVVETWGVDMRGW"
BASE COUNT         1142 a         2951 c         2796 g         1077 t
ORIGIN      
        1 gtcgtcgaag aagccgcggt cgtaggcggc agcgaggttg cggtggctcg cggccgccag
       61 ctcgtcctgc tcctcgcgtc cgacgtgcca ctcgagcgcg gtgagcgcgg catgctcgcc
      121 catcgacagc ccggtgcgcg gttccgcgtt gcgcgggatc tccgggacga tctgtccggg
      181 tcgcagcccc cgcagcgcct tcaggcgcgc acccagcgac cgcgccccgt tgagctccat
      241 caggacccgg cgcaggtcgt cgttcaggga gacgggcgca tcgctcgccg tgtcgacacc
      301 ggcggcgata ccgctctcga tctgcccgag ggcgatcgtg ttggcgacga ggatcgcggt
      361 ctccaagccc gtaccgcagg cctgctgcac ctcgtacgcc ggcgtgtagg gcgagagggc
      421 gctgcccagc acggcctcgc gggtgaggtc acgatctcgg ctgtgtttga gcaccgcgcc
      481 gcctgcgacc tcgccgagcc gctcgccggc cagtccgaag cgcgcggtga gcccgtcgag
      541 ggtggcggtg agcatgtcca ggttggacgc gcgggcgtag gcgccgttcg cgcgggcgaa
      601 cgggatgcgg ttgccgccga tcacggcggc tttccgggtg tccggcacgg gttcctcccg
      661 ggtgaggtgc gtttggacgg cgttttaccc atcagtagcc gatactcaaa gtacacggta
      721 ctctcggttc cgtgaaggtg gtccgggaca agcgcaccag ccggtgggac gcccatcggc
      781 gcacgcgccg cgccgagctg accgatgccg cgattgccgc gatccgtgcc cacggcgccg
      841 gcgtcgggat ggacgaggtc gcagccagcg cgggcaccag caagtccgtc gtctaccggc
      901 acttcactga ccgcacccag ctctacgtcg cggtctgcgc gcgagtggcc gaggtgctgg
      961 tcgctcaggt gcgcgccgcg atgaacgacg ccgacgggcc ccgcgccaaa acggccgccg
     1021 gcatcgccgc gtacctgcgg ttgatcgaac acgacccgga ggtctaccgg ttcgtcgtgc
     1081 accgcccact cgtcgatcgg gtactcgacg cggatccggt cgccgacctc gtctcgctca
     1141 tcggcgacga agccgcagcg gtgatcgcgg cgcagctgcg ccacaccggc tccgacacct
     1201 ccgccgccgt gccgtggggc cacggcatcg tcgggctcgt acgcgctgca gcagacaact
     1261 ggctggcccg acctgcgggg atcacccgcg aggtgctggc cgcacagctc accgacctgg
     1321 cctggtccgg gctctccggc gtcgtggctc cccgcccggc catcccgaag gaggaactcg
     1381 catgacggtg gaccacagcg aactccagcg cgtcctcgac ggccgatggg ccgacgtccg
     1441 ggccgaagtt cgcgcgcagc tcggcgcgct cgcgctcaac cccgaccccg acctgagcac
     1501 cgaggagtac cgggcgctga ccgccgagaa cctgcgacgg ctggccgaga gcggccgccc
     1561 gcaccagggc ttcgacccgg cgtacggcgg cgggggcgac gtcggcgggg tcgtcacggc
     1621 attcgcggcg ctgggctacg gcgacctctc gctgctggtc aaagccggcg tgcagtgggg
     1681 gctcttcggt ggggccgtcc aggtgctcgg cacttctcgt catcatgaca agtacctgcg
     1741 ctcgatcatg gacggggacc tgctcggctg cttcgcgatg accgagaccg gccacggctc
     1801 cgacgtccag cacctgcgta ccaccgccac ctatgacccg gacaaccacg agttcgagat
     1861 ccacacgccg gatccagggg cgcgcaagga ctacatcggc aacgccgcgc gggacgggcg
     1921 gatggccgtc gtcttcgcac agctgatcac cggcggcacg tcccatggcg tccacgccct
     1981 gctcgtgccg atccgcgccg acgacgacac gaccatgccc ggcgtcacga tcggcgactg
     2041 cggtcgcaaa gctggcctca acggcgtcga caacggcagg ctctccttcg accacgtccg
     2101 cgtgccgcgg gagaacctgc tcaaccactt cgccgacgtc gccgaggacg gcaggtactc
     2161 cagttcgatc gaaagttcct cgcgccggtt cttcacgatg ctcggcacgc tcgtccgggg
     2221 ccggatcagc gtggcgggcg gcgcaggcac ggccacgcag aaggcactcg cactggccat
     2281 ccgctacggc gcgacacggc gccagttcgc cgaccccggc accggcgagg aggtggtcgt
     2341 gctcgactac ctcgtccacc agcgcaagct gctccccgcg ctcgccacga cgtatgcgct
     2401 gcacttcgcg caggccgacc tcgtctcgga catgcacgat ctgcagtcgt tcgacgcgga
     2461 gcagggctcg gacagtgaca ccgagcgacg gcagcgcgac ctggagacca gggccgcggg
     2521 catcaaggcg ctggccacct ggcacgccac caccacgatc cagacctgcc gcgaggcgtg
     2581 cggcggcgcc ggctacctcg cggagaacct gcttccgcag ctcaaggccg acaccgacgt
     2641 gttcacgacg ttcgagggcg acaacacggt cctgctgcag ctggtggcga agacgctgct
     2701 ctcggactac gggcgccggg tcgggaagct cgacctcatg ggcaaggcgc ggttcgctgc
     2761 gggcctcgtg gccggctccg tcgccgagcg caccggcctg cgggccgggc tccgccgcgg
     2821 ggacgtgcgc gaccgcgcct accagcgatg gctgctcgtc cagcgcgagg agcacctgct
     2881 ggccgctgcc gcgcggcgga tgcgcagggc actggccccg ggagccgacc agttcgccat
     2941 cttcaacggc gctcaggacc acctgttggt ggccgcacag gcgcacgtgg accggatcgt
     3001 cgccgactcg ttcgccgccg ccgtcgaccg ggtcgacgac acagccgtgc gagcccttct
     3061 ggatcaggtg cgcgggctgc acgcgctctc ggtgatcgag ggcgaccggg cgttcttcgt
     3121 ggagcacggg tacctcacgc cgggccgggc caaggccgtc acccacgccg tcagcacgct
     3181 gtgcgggcag gtccgtccga acgccaggac gctggtggac ggtttcggca tcccggaggc
     3241 ctggctcgcc tgcccgctgc tcgacggcga gcgcgacgcg acggccgcgg cgaccgatcc
     3301 cgctccgtcc acggaggagc cgaccgacct ggccgcggtg ggctgagctc cgcgcgccgg
     3361 ccaggttgag cgaacggcac tttcggccga tagggtccgg cccaacgcgc ctttcgagcg
     3421 accggagcgg cggggcgacc gcgggggcgc aagacaccgc accggcactt cagcgcccgt
     3481 ccgcagcaag ccgcatgtgc cgcggtggtg ccctcgtcca cgccgaacgc gatcagcgcc
     3541 gggagcgccg cacagcggtt cagttgacag gccggcccgc ggtgagcagg cgcggcccgt
     3601 cgtcggtgat cgcgatcgtg tgctcggcat gggcggcccg gctcccgtcg gccgtgcgca
     3661 aggtccagcc gtcggggtcg gggctgtagt cgtccgcccc gccggcgagc agcatcggtt
     3721 cgatcgcgat caccagcccc ggccgcagcg tgaacccccg gcccgcgcgg ccctcgttgg
     3781 gcacgtgcgg ggcctcgtgc atagcccgcc cgacgccgtg gccaccgtga ccggccagta
     3841 gcccgtaccc gcccgcacgc ccgacgaccc cgatcgcatg cgcgacgtcg cccatccggg
     3901 cgcccgatac cgccgcggcg atgccggcgg ccagggcccg ctccgtggtc tcgatgagtg
     3961 cgacgtcgag cggatcggct tggccgacca ccgtgctgaa cgccgcgtcg gcgcaccatc
     4021 cgtcgaggtg caccgcgagg tcgacgctga gcaggtcgcc atcgcgcagc cgtccccggc
     4081 cggggatccc gtgcaccacg gcgtcgttca cgctggcgca gatcacagcc gggtacgggg
     4141 tcggcgcgaa gctcgggtgg tacccgagga agctcggccg ggcgccggtg ccggcgatca
     4201 ggtcggcggc gagcgcgtcc aactgccgag gcgtgacgcc gggagcggcg tgttcacgca
     4261 cggcggaaag gacgtcggcc acgatctttc cggcggcctc gacagcgtcc agctccgccg
     4321 gcgtcttcaa ctcaatcatg ccgataacta taccggtatt actatcgggg tcatggtgcg
     4381 cgttcccctg accccggccg accgcgagcg cggggagcgg ctcgggcggc tgctgcgcga
     4441 gtccagggcc gaccgcagcc tggccgacgt cgccggcgcc gccgggatct cggcggagac
     4501 cctgcgcaag atcgagaccg ggcggattcc cacgcccgcg ttcttcaccg tgtcggcgct
     4561 ggcaggggcg ctcggcctgt cgctggacac cctcgtcacg gccacggcgc caccactccc
     4621 ggaagaccgc gcggccgtct gacggctcgc cgatacgaat cgcccggtgc cagcgcacgg
     4681 atctcgggtg tcagtcagcc cagtgaccgc gctcacatcc ggcaactgct ccgccccccg
     4741 tatgtgccga agcacgggcg atcacctcct cgagggtgag cgcgctcgcc gggtgatggc
     4801 tcaggcacat gggttcctgc accttctcga acgccaggcc ctcgctcgcg acgtagaact
     4861 ggccggatct gagctgggag atgtcgctca cctgaccgcc cttggccggc ctccgaggtc
     4921 gtggtcgtcg agggacatcg atccggatgg tgtcatcgag tccgaaccgt cccggcagcg
     4981 cggtgacctt ggcgtggcag gtcaggcgac ctcgatcgcc accttcccgc gcacgtgccc
     5041 ggcctcgacc gtggcgagcg cggcaccggc ctcgtcgagg ggacggatct cggtgacgag
     5101 cgggtcgagc tcaccctcgg cgacgagttg cgcgagcgtg atgagcacgg ctgtgctgcg
     5161 gtcgcgctgg acaccgctac cacccagccg ggccgcgagc gggcggtctg cgatcgtcac
     5221 cagccgggac cggtcgggca gcacctcggc aacggctcgc agggcgtcgc cgccgacggt
     5281 gtcgaacacg gcgtcgacgc cctggggcgc ggcggcccgg atccgctcgg ccacgccgtc
     5341 cccgtagacc accggcgtgg cgccgtagga ggccacgagg tcgtgcttgc cctggctcgc
     5401 ggtgccgaca accacgagtc cgcgggcgcg ggccagctgc agcgtcgcca ctcccacccc
     5461 gccgccggcc ccgttgacga gcaacgtcga gccgggcgcc aggtcgagcc cggtcagcgc
     5521 gtcgtaagcg gtggcgggtg ccaccgggag cgtcgcggcg tcggcaggcg ccacccgctc
     5581 cggccggtgc gcggcgaacg aggccgtcac cagcgcgaac tcggcccatc cgcccaccat
     5641 gccgggggtg cccccgaaca cctcgtcgcc gacggcgaag ccgtcgacgc ccggcccggt
     5701 cgccacgacg ctgccggcca cctcccggcc gagcaccgcc ggcagcgcca catcgccgta
     5761 cccgccgtcc cgcagcttcc agtcgcccgg gttcactccc gcggcacgga cccgaacgag
     5821 cagctcgccc ggtcccggct cggggaccga caggtcgagc atcgcctcgt tctcggggcc
     5881 gccgaacgcg gtgacgccgt aggccctcat gcccgcaccg acgcccgcag agcggccagc
     5941 cgggcgacgg cctctgtgag cacctcgtcg cgtttgcaga acgcgaagcg caccagcgag
     6001 cgcccgaggt cggggtcggc gcagaacacc gacaccggga cggcggccac gccgacccgc
     6061 tccggcaggc tccagcagaa ctcggcgccg tcggtgaacc cgagcgccgt gacgtcggtg
     6121 acgacgaagt acgtgccggc cgtgtcgaac acggtgaagc ccgactcgcg cagcccgtcc
     6181 gacagcagat cgcgcttgcg ctgcaggtcg ccggcgagct ccgtgtagaa ggagtcgggc
     6241 agcgcgaggg ccgcggcgat cgcgggctgg aacggagccc cgctcacgta ggtgaggaac
     6301 tgcttcgccg cgcgcaccgc cgacacgagc tcgcccggcc cgtgcaccca gcccaccttc
     6361 cagccggtga ccgagaaggt cttgcccgcc gacgagatcg tcagcgtgcg ctcggccatc
     6421 cccggcagcg tggccatcgg gatgtgctca cgtccgtcgt agatcatgtg ctcgtacacc
     6481 tcgtcggtga cgatcacggc accgtgctcg gcggccagct cgccgatcag cgtgagctgc
     6541 tcgcggtcga acaccgcgcc cgtcggattg tgcggggtgt tgaccagcac gatccgagtg
     6601 cgttcggaga acgcggcgcg tagctcatcg gggtcgaacc cgaacgacgg gggccgcagc
     6661 gccaccgctc ggagctgcgc tccggccagc gcgatggtgg ccgcgtacga gtcgtagtag
     6721 ggctggaacg tcaccacctc gtcgcccggc tcgcagagtg cgagcaccgc ggaggcgatc
     6781 gcctcggtcg ccccggtggt gacgagcacc gtgtcggggt cgacatcgag gccgtagaac
     6841 cggcgttgat gctcggccac cgcggaccgg agctccggga caccgatacc cggcgggtac
     6901 tggttgaccc cggcctggat gttcgctgcg gcgtcgctga gcagcgaggc cgggccgtcg
     6961 gtgtcgggga agccctgacc gaggttgatc gcccccgccc ggagggcgag ggctgacatt
     7021 tcggcgaaga tcgtcgatgt atgagggcgc atccgctcga ccagcatgat gaaacgttag
     7081 ctgccggccc tcctacgcag cggtgagcca gccgcggcgg atccgcgtcg agccgaccat
     7141 cgtcctcggg ctgtacgagg tcgagctcac cacccgcgca tgtcgacgcc ccaggtctcc
     7201 acgacccggt ccccgtccac cacgtgcatc tgctcgtgca gcaccacggt cgggtcgacg
     7261 tgcgccggca gcacccggac gcggtcgccg acccggacgg gttcctcggg cgcgaacgac
     7321 gtgtgctcgt cggagcagaa ccacacctgc gcgccgggga tcgccgggag accgtggtcc
     7381 atcccgaggg acttgagtcc gacatcgacg acggcatgtg tcggcgctac ggagacgatg
     7441 gtgccgagca ccgacaacgc ctgcccgaac ggctgcccga cagcgccgta ggccgtgtcc
     7501 atgagcgcgt acgaacccgc ctggatctcg gtggcccagg tgttgtcggc gtacgttccc
     7561 gtgccgcctg cactgatcag ctcaccgccg acttccgcgg ctgcggcaac gagcagcgcc
     7621 atggcctccc cggtcatccg ggcacggtct gccgggtcgg ccacgagcat gaggtggccc
     7681 tcgtagccca tgacaccgcg cacctccagc ccgccgcgcc gcgcctcacc ggccaggtca
     7741 cctgcccgct cgggcgggca gccgcagcgc ggcagaccga cgttcacgtc gatgagcacc
     7801 tcgcgcacac cgccggccgc agccgccgcg atcgtctccg gcgagtcgac ggcgaccgtg
     7861 attcgcgccc cgtcgcgcac gagtgtcccg agcctgcgcg cgtcaagcac ctcgttggcg
     7921 agcaggaggt cctcgccgag gccagcggcg accatcgcct cgcact
//
LOCUS       DASRSC010000112        14978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3637218,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000112 DASRSC010000000
VERSION     DASRSC010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 14978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14978
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3637218"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1861)
                     /locus_tag="VFE39_05310"
     CDS             complement(110..1861)
                     /locus_tag="VFE39_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HET6258053.1"
                     /translation="MTTTGLIALFAMGLVALASAVIGSLGVRVVRSTSDFLVASRTVG
                     PAANAGAISGEYLSAASFLGIAGLILRDGADALWYPVGFTAGYLALVLFVAAPLRRSG
                     AYTVPDFAEARLRSPLLRKICTIFVVIIGWLYLLPQLQGAGLTVTTVTGLPTWTGIVA
                     AAVVVVATVVLGGMRSITFVQAFQYWLKLTALALPAIIALIFFLSDQRSFDRPAPPQF
                     PDRTTVDIRTDVVLQVVTPVNFIASGELDGVPHAAAPVRWEVGTHTVEAGAVLEFPGG
                     SAVPVVAGAPATDESWLAPFPKGRDDGLLATYSLILALFLGTMGLPHVLVRFYTNPDG
                     RAARRSAVVVLALLGAFYLVVTLLGALSRLYTPQLLVNGDTDAAVLLLPGAVLGSGWA
                     GSLLGGLVAAGAWAAFLSTSSGLVVSVAGVLSTDILGSSRVRDFRMSAVVAGAVPLGL
                     SVAVTRLDFSEAVALVFAVAASTFCPLLVLGVWWRGLTDVGAIAGVLVGGLTSAGAVA
                     VSLSGLGGPGWIGVLLYRPALATVPAAFLTMVVVSRLTAKHRPADVDQVLLRLHAPER
                     LGLTRDRLEERVKRASG"
     gene            complement(1999..3729)
                     /locus_tag="VFE39_05315"
     CDS             complement(1999..3729)
                     /locus_tag="VFE39_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HET6258054.1"
                     /translation="MSTPALVALVAIALVGIVSAVIGSIGVRIARSTSDFLVASRTVG
                     SVANASAISGEYLSAASFLGIAGLILREGADALWYPIAFVAGYLALVLFVAAPLRRSG
                     AYTVPDFAEARLGSPALRRMCTTFVLAIGWLYLLPQLQGAGLALTTLTSLPSWSGVAA
                     ASVLVVLTVIGGGMRSITFVQAFQYWLKLTALIVPAIVVLGLFMSDARSFDRPAPPNF
                     PHDTTVQVRTDVVLEVTATVRFEAAGEIDDQPAHGRVTWEPGQHEVAAKTTLTFAAGS
                     AVPTVVGAPTTDRAWLEPMSGDAPDQLLEIYSILVAGFLGTMGMPHVLVRFYTNPDGR
                     AARRTTVVVLAMIGFFYMLVTFVGALSRLYTPELLVSGQTDAAVLLVPTAALGNGWTG
                     WLLGGLVAAGAAAAFLATSSGLVVSLAGVLFTDLLRGRLRDFRLAALVASIVPLPVAL
                     SVTRLDFALTVPLVFAVAASTFCPLLVLGIWWRGLTAPGAIAGLLVGGGLSGIAVAIT
                     MFAVLPSGWLGVLLYRPAAITVPAAFLTMVVVSKLTRAEIPPGVDGTLLRLHAPERLG
                     LTHDRLDREA"
     gene            complement(3739..4944)
                     /locus_tag="VFE39_05320"
     CDS             complement(3739..4944)
                     /locus_tag="VFE39_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258055.1"
                     /translation="MTTFTDGVPPLVTPEPGDGPAPSGHGVAQGDKPPHHAETSAEHC
                     DNDDADDGMDTGSGWLRDEIQRRIAAGHSGTGGRHARRESVDSSTSLGYVPRHSVATP
                     GPGAPRPGPVGGPVGLRPTGLLRRERTGAEGPFLPIAPAAWSRPARPDTAGTAPPSVD
                     ADPPDTPPAPPAPDLRAEAALEHPVPPAPDLRADAAPEYPVPPAPVLPVTAPPAYPLR
                     GAVPTALVGTVPPIGSVSVPALSGDPLFTARTPAPGSRERGSSLAVPEQRGVDEPHGG
                     PESQDDVESRRVRVVLAERKGVARPVRTVVDIQEGTGVGELLRRNLIGSQLAVALRFA
                     VGAGLTLGVLPLLFAMFPELGRMEVLGLRLPWLLLGVLVYPFLFGLGLWHARTAERVE
                     QNFADHVQD"
     gene            complement(4941..5813)
                     /locus_tag="VFE39_05325"
     CDS             complement(4941..5813)
                     /locus_tag="VFE39_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HET6258056.1"
                     /translation="MDSNDSSGGLVVLAVDDEEPALEELAYLLGEDSRVGIVLKASDA
                     TDALRILNSRQGVREAATAGQVSRGLANPMTGTRVAERTDVEVVFLDIRMPGLDGLEL
                     ARVFSSMAVPPSVVFVTAHDDRAVDAYEVGAVDYLLKPLRAERLAASIDRILANRTTG
                     APEPVREEGGEDEVIPVELAGTTKLVPRSAVRYVEAQGDYARLHTHEGSHLVRIPLSV
                     LEDRWRDAGFVRIHRSFLVSLPLVTELRLSGSGYVVRVGNGPDCAELPVSRRHTRELK
                     DRLVRATKQAWSQR"
     gene            complement(5950..6834)
                     /locus_tag="VFE39_05330"
     CDS             complement(5950..6834)
                     /locus_tag="VFE39_05330"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HET6258057.1"
                     /translation="MDALRLPDRLTSRLPGRLGDRTDRPVVSMVRLHGVITPTTGPVP
                     RSVINANAVEKTLERAFAPDGLAAVALLVNSPGGSPTQSALVADRVRGLAEEKGVPVL
                     AFCEDVAASGGYWLACAADEIYAHATSIVGSIGVVSQGFGLHGLIERFGVQRRLYTAG
                     TSKARLDPFLPEKQEDVDWLRDLQHELHEMFKQWVLERRGDRLKTAAEPFTGEVWTGA
                     RAVEIGLVDGLGTARGVLGKRFPEAELVPVEGRRPLLARLGLGAVPAAAGATLGADHL
                     LGITQAAEIRATWARYGL"
     gene            complement(6905..8110)
                     /locus_tag="VFE39_05335"
     CDS             complement(6905..8110)
                     /locus_tag="VFE39_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6258058.1"
                     /translation="MAELLGSSTALLIAGAVIVFMIVLVVVLILRNRRNTFATPLERA
                     TFATLHTVALAAPSLREGLSHSSAAFALPYLRQLLDTCALTMTDNRGETLAWDGDADQ
                     HEPDVRALADQVISTGRQQVLSHTAIICDHPDCVVRGIVVVPLETDGAIVGTLAALTT
                     STAGPVLLRATAEVARYVSSQLELAELDESRARLNRAEVRALRAQISPHFVYNALTTI
                     ASFIRTDPVRARELLIEFADFTRYSFRTAGEYTTLSDELTNIERYIRLEKARFGNRLN
                     IKLQIAPEVLSVVLPFLALQPLVENAVRHGLASKPNGGTITITAENAGAECVIIVEDD
                     GIGMDPARLTDDLDDAHQSGAHVGLGNVDDRMRSTFGDDFGLVVDTAPGAGMKITLRV
                     PKFRAGIRV"
     gene            complement(8118..8993)
                     /locus_tag="VFE39_05340"
     CDS             complement(8118..8993)
                     /locus_tag="VFE39_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET6258059.1"
                     /translation="MPELPEVEALAHHLREHALYRPVARVDVASMSAVKTFDPPVSVL
                     PGRVVTGAARYGKFLAVEFMDRPDDPLHLVTHLSRAGWLRWHDSASATPPKPGRGPLE
                     LRVHLDAVGGPGFDLTEAGTQKRLSVYLVHDPQEVPGIARLGPDALTLSRDEFAAVLA
                     GRSERLKTLLVEQQTIAGIGNAYSDEILHTAKLSPYAASGRLKPEQIDELYAAMCSVL
                     ADAVERSVGQGAAQLKGEKRSGLRVHARTGMPCPVCGDTVREVSFASKSFQYCPRCQT
                     GGKPLADRRLSRLVR"
     gene            complement(9010..9435)
                     /locus_tag="VFE39_05345"
     CDS             complement(9010..9435)
                     /locus_tag="VFE39_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258060.1"
                     /translation="MAGVDGRMWLVRRNIEWSLPATGDDFEHDVDGGRGAAVLILSAL
                     FFFWLIVIVWAPADVHIPWFLWLLGAVVVLFFPVRWYLRRPWTVVAETAGNYDLPAEQ
                     WTGLVRGGAKSKEELRIVVRRLRTQGTPGHADSPLQPVN"
     gene            complement(9521..10021)
                     /locus_tag="VFE39_05350"
     CDS             complement(9521..10021)
                     /locus_tag="VFE39_05350"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6258061.1"
                     /translation="MSRTGPRLAAAMGLAALLLTGCAQPGQLGSAPEPVPTPLPPPPP
                     APAPAPPAGPVLVATGRVEERVTMRTTGPASYSVRTVVLQPGESTEWHRHPGTELSIV
                     QSGQVTVLREDDCAPTRYSRGDAVFFADGQRHLARNDGAVPAELVVTYLLDPGAPVRS
                     AVLPAC"
     gene            complement(10062..11288)
                     /locus_tag="VFE39_05355"
     CDS             complement(10062..11288)
                     /locus_tag="VFE39_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6258062.1"
                     /translation="MLDVFDPRTFARGVPQDDLRLLRDTDPVSWQDEPEVGIWPAGPG
                     FWAVTRYDDVRHVLRSPADFSSSLGATQIRDPDPADLPFIRRMILNMDPPEQVRLRRL
                     VTGAFTRRRLERFTQVIQERAGALLAGVAGERRCDLPRDVTDDLPLQNLADLLGVPAA
                     DRELLLEWTNRVIGYQDPEHGQIVTDAEGRPVNPRSPAMLGDMFGYAEALADHKRREP
                     ADDVMTALVEAEVDGERLTDAELKMFFFLLVVAGNDTVRSALPGGVLALVQNPDAYRR
                     LRADPALLPSAIEEMLRVHPPVLTFRRTATRERELGGRRIAAGDKVVVYHISANHDER
                     HFPDPYRFDIERDPNEHLAFGQGPHLCLGATFARLQMRVFFTEFLRLSPVELDGEPRR
                     LVSNFINGITSLPLRW"
     gene            11471..11932
                     /locus_tag="VFE39_05360"
     CDS             11471..11932
                     /locus_tag="VFE39_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6258063.1"
                     /translation="MGEDDLVVADRIAQELGQLTRMVTSLRRHRGDIAVAHVLVQLAE
                     QGPLRVSAIADAVGSDPSTVSRKATELVAAGLVERRPDPGDGRAHLLAVTEAGARCCE
                     DGRRKRIELVSAVLSGWSDESRQRLAELLGRFADDVQELDRRVSRRSGGEN"
     gene            11932..14775
                     /locus_tag="VFE39_05365"
     CDS             11932..14775
                     /locus_tag="VFE39_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET6258064.1"
                     /translation="MSTQVAVEPITRAEPPPSGELTHRQVLTILVGLALGMFLAALDQ
                     TVVSTAIRTIADDLGGLSLQAWATTAFLITGTITTPLYGKLSDIYGRKPLFLTAISIF
                     IAGSVMCTFAQSMYELAAFRAVQGLGAGGLFSLALTILGDIVPPRQRARYQGYILAVF
                     GTSSVLGPVIGGVLSGQATIAGIAGWRWIFLVNVPIGAIALIVVARVLTMPHIPRPHR
                     IDWPGALMLAVFLVPLLVVAEQGQEWGWGSTSSIVCYVIGVVGLILFLIAERSYGDDA
                     MLPLRLFRTSVFALSAGAGILIGAGMFGGISLLPQFLQIVHGASPTEAGFLTLPLVAG
                     IMVSSIVSGQITSRTGRYKIFPIIGTALMVGAMLLMYFRVAVDIPLWELDLYMAMFGL
                     GLGLSNQTLVLAVQNAVPARDMGVATASTTFFRQLGGTVGVAAFLSIVFNTVTGNISD
                     EFSKAATTPEFQAALADPAVRANPANQPVLAALTGGGNSGASSVLTNSSFLQTIDARL
                     ARPFLAGFADSMTMTFLIVACVLAVAFVIVLFVKELPLRTMSGAQARAMEDAAMAEAG
                     PAGSTLFAEAAVAGAASSATPNGVGGPGPNGAGRHALLTHEALPAAVGSNGYGNGHAV
                     GNGKPAPRPSPTPRHDPGAVTYPATYPATMLPEGAHADYNGPAVYGTVTRSDGVPLAQ
                     VVITVADPAGRQEARTAAGPDGYYRVDLRNGGTYLVVAAGGTYQPHAAMVAVGDAPAR
                     HDVMLSGTSSVIGTVLTPDAAAGSRTVGGAAVTLIDMRGDVAASRVTDADGRYQLVGV
                     PDGSYTLTAASPGYQPIAVSVRLDVGATVECDLELPQRSQLLGMVTAVSNGRGVPEAM
                     ATLVDATGTVVGSTVTRPDGSFVFDDLAEGTYTLTASGYAPVAQLVHVTAGAQASTVV
                     ELGTPAPAEAPSTAPPAEQQFDVFEPFDAGGVR"
BASE COUNT         2370 a         5244 c         5344 g         2020 t
ORIGIN      
        1 gcggttccct caccaagacg agcggtctac gaacgggaaa agcgttgaca gagcgttacg
       61 gatgtgacag ataggtcact caatcgttag cgcttcggta tgtgtgttgt caaccgcttg
      121 cgcgtttcac gcgctcctcg agccggtcac gggtcagccc gagccgctcc ggtgcgtgca
      181 gccggagcag cacctggtcg acgtcggcag gccgatgctt ggccgtcagc cggctcacga
      241 cgaccatcgt gaggaacgcg gccggcaccg tggccagcgc cggccggtag agcagtactc
      301 cgatccagcc cgggccgccc aagccggaga gactgaccgc caccgctccg gccgatgtga
      361 gtccgccgac cagcacaccg gcgatcgcgc cgacatcggt cagcccccgc caccacacac
      421 ccagcacgag cagcgggcag aacgtcgacg cggcaacggc gaacaccagc gcgacggcct
      481 cggagaagtc gaggcgggtg accgcgacgg agagcccgag cggcaccgcc ccggcgacga
      541 cggcggacat ccggaagtcg cgtaccctgc tgctccccag gatgtccgtg gagagcacac
      601 cggcgacgct gacgacgagg ccggacgagg tggacaggaa cgctgcccag gcacccgccg
      661 caaccaggcc gccgagcagg gatccggccc agccgctgcc cagcacggcc ccgggcagga
      721 gcagtaccgc cgcgtccgtg tcgccgttca ccaggagctg gggcgtgtag agccgggaca
      781 gcgcgccgag cagcgtcacc accaggtaga aggcaccgag cagagccaga acgaccacgg
      841 cgctgcggcg cgccgctcgg ccgtcggggt tcgtgtagaa gcgcacgagc acgtgcggca
      901 ggcccatcgt gcccaggaac aacgcgagga tcagcgagta cgtagccagc agcccgtcgt
      961 cgcggccctt cgggaacggg gccagccagc tctcgtccgt cgctggggcg cccgccacca
     1021 ccggcactgc cgatccgccg gggaactcga gcaccgcacc cgcctcgacc gtgtgcgtgc
     1081 cgacctccca ccggacgggc gcggcggcgt gcggcacccc gtcgagctca ccactcgcga
     1141 tgaagttgac gggggtcacg acctgcagca ccacatcggt acggatgtcg acggtcgtcc
     1201 ggtccgggaa ctgcggaggg gccggacggt cgaaggagcg ctggtcgctg aggaagaaga
     1261 tcagggcgat gatcgccggc agcgccagcg cggtgagctt gagccagtac tggaacgcct
     1321 gcacgaaggt gatggaccgc atgccgccca gcaccaccgt ggcgacgacg accaccgcgg
     1381 ccgcgacgat accggtccac gtcggcagcc cggtgaccgt ggtgacggtg agacccgcgc
     1441 cctgcagctg gggcaacagg tagagccacc cgatgatgac cacgaagatc gtgcagatct
     1501 tgcgcagcag gggcgaacgc agccgggcct ccgcgaagtc gggcacggtg taggcccccg
     1561 accgccgcag cggcgccgcc acgaacagca ccagagccag gtagccggcg gtgaagccga
     1621 ccgggtacca gagcgcgtcg gcgccgtcgc gcaggatcag ccctgcgatg ccgaggaagg
     1681 acgcagcgga caggtactcg cccgagatcg cgcccgcatt cgcagcggga ccgacggtgc
     1741 gggacgccac gaggaagtcg gacgtgctgc ggaccacccg cacgccgaga gaaccgatca
     1801 cagccgacgc cagcgccacg aggcccatcg caaagagcgc gatcagacca gtcgttgtca
     1861 cgcataccac tatgtcaacg cggaggccgc actaccacat caccggcgcg ccgcccgcag
     1921 cgcgtcgggg tgaaccagga gagcctcgct acttgcggag cgggtcctgc aaccccggcg
     1981 ggcccggtga tcgcgcagct aggcctcgcg atcgagccgg tcgtgggtga gcccgagccg
     2041 ctcgggagcg tgcaggcgca gcaatgtccc atcgaccccc ggcgggatct cggcccgggt
     2101 gagcttgctc accacgacca tcgtgaggaa cgcggccggc accgtgatcg cagcgggtcg
     2161 gtacagcagg accccgagcc aacccgacgg cagcaccgcg aacatcgtga tggcgacggc
     2221 gatcccggac agccccccgc ccaccagtag cccggcgatg gcgccgggcg ccgtcagccc
     2281 gcgccaccag atcccgagca cgagcaacgg gcagaacgtc gacgccgcca ccgcgaacac
     2341 cagtggcacc gtcagcgcga agtccagccg ggtgaccgag agggcgacgg gcagcggcac
     2401 aatcgacgcc accagcgcgg ccagccggaa gtcacgcagc cgcccccgga gcaggtcggt
     2461 gaacagcacc ccggccaggc tgacgacgag ccctgacgaa gtggccagga acgcggcggc
     2521 cgccccagcc gccaccagac cccccagcag ccacccggtc cagccgttgc cgagagccgc
     2581 cgtcgggacg agcaggaccg cagcgtcagt ctgcccgctt accagcaact ccggggtgta
     2641 cagccgggag agggctccga cgaacgtcac cagcatgtag aagaacccga tcatcgccag
     2701 cacgacgacg gtcgtgcgcc gggcggcccg gccgtccggg ttggtgtaga agcgcaccaa
     2761 cacgtggggc atccccatcg tgccgaggaa gccggcgacg aggatcgagt agatctccag
     2821 cagctggtcg ggtgcgtccc cggacatcgg ctctagccag gcccggtcgg tggtcggggc
     2881 accgaccacg gtcggcacgg ccgaaccggc ggcgaacgtc agcgtcgtct tggccgccac
     2941 ctcgtgttgt cccggctccc acgtcacccg gccgtgcgcc ggctgatcgt cgatctcgcc
     3001 cgccgcctcg aagcggaccg tcgccgtgac ctccagcacg acgtcggtgc gcacctgcac
     3061 cgtcgtgtcg tggggaaagt tcggcggggc ggggcggtcg aacgagcgtg cgtccgacat
     3121 gaacaggccc agcacgacga tggcgggcac gatcagcgcg gtgagcttga gccagtactg
     3181 gaatgcctgc acgaaggtga tcgagcgcat gccgccgccg atcaccgtca gcaccacgag
     3241 cacgctggcc gccgcgacac cgctccagga agggagggag gtgagcgtgg tgagcgcgag
     3301 gccggcgccc tgcagctgcg gcagcaggta caaccagccg atcgccagca cgaacgtggt
     3361 gcacatccgg cgcagcgccg gtgagccgag ccgggcctcg gcgaagtccg gcacggtgta
     3421 cgcacccgac cggcgcagcg gcgccgccac gaacaacacc agagcgaggt agccggcgac
     3481 gaacgcgatc gggtaccaga gcgcgtcggc gccctcccgg aggatcaggc ccgcgatgcc
     3541 gaggaaagac gccgccgaga ggtactcccc ggagatggcg ctggcgttgg ccacgctgcc
     3601 gacagtgcgc gacgcgacga ggaagtcaga cgtgctccgt gcgatccgga cgccgatcga
     3661 cccgatcacg gcggacacga tgccgacgag cgcgatcgcg accagcgcga cgagcgcggg
     3721 agtactcacc gttcgagctc agtcctgcac gtggtcggcg aagttctgct ccacccgctc
     3781 ggcggtgcgc gcgtgccaga ggccaagccc gaacaggaac gggtagacca gtaccccgag
     3841 cagcaaccac ggcagacgca gcccgagcac ctccatccgc ccgagttcgg ggaacatcgc
     3901 gaacagcagc ggcagcaccc cgagggtgag cccggcgccc acggcgaagc gcagcgccac
     3961 ggccagctgc gaaccgatga ggttcctgcg caacagctcg ccgacgcccg tgccctcctg
     4021 gatgtcgacc acggtgcgca ccggccgggc cacgcccttg cgctcggcga gcacgacccg
     4081 cacccgcctg ctctcgacgt cgtcctggct ctccggaccg ccgtgcggct cgtcgacgcc
     4141 gcgctgctcc ggtacggcga gcgaggatcc gcgttcgcgc gagccggggg ccggggtgcg
     4201 tgcggtgaac agggggtcac ccgagagcgc cggaacggag actgatccga tgggggggac
     4261 cgtcccgacg agcgcggtgg gaacggcgcc gcggaggggg taggcgggcg gggcggtgac
     4321 gggcagtacg ggcgccgggg gcacgggata ctcgggggcc gcgtcggcac ggaggtcggg
     4381 cgccgggggc acgggatgct cgagggccgc ctcggcacgg aggtcgggcg ccgggggggc
     4441 aggcggggtg tccggagggt cggcgtcgac gctcggtggc gcggtgccgg cggtgtccgg
     4501 acgggcgggt ctcgaccagg ctgccggtgc gatcggcaag aacggcccct ccgcgccggt
     4561 gcgttcgcgc cggagcagtc ccgtgggccg gagcccgacg ggcccgccga cgggtccggg
     4621 ccggggcgcg ccgggacctg gcgtggcgac ggagtgccgt ggcacgtacc ccagcgaggt
     4681 ggacgagtcg acgctctcgc gtcgggcgtg ccgccctccc gtgcccgagt ggccggccgc
     4741 gattcggcgc tggatctcgt cgcgcagcca cccggagccg gtgtccatgc cgtcgtccgc
     4801 gtcgtcgttg tcgcagtgct ccgcggacgt ctcggcgtgg tgaggcggct tgtcgccctg
     4861 tgcgacgcca tgaccgctgg gcgccggacc gtcccccggc tcgggcgtca ccaacggcgg
     4921 gacgccgtcg gtgaacgtgg tcaccgctgg ctccacgcct gcttcgtcgc gcggaccagc
     4981 cggtccttca gctcgcgcgt gtgccgacgg ctcaccggca gctcggcgca gtcgggcccg
     5041 ttcccgactc ggacgacata cccggagccg gacagccgca gctcggtgac gagcgggagc
     5101 gacaccagga acgagcggtg gatgcgcacg aagcccgcgt cgcgccagcg gtcctcgagc
     5161 accgacagcg ggatccggac gaggtggctg ccttcgtggg tgtgcaggcg ggcgtagtcg
     5221 ccctgcgcct cgacgtagcg cacggccgag cgcggaacga gcttggtggt gccggcgagc
     5281 tcgaccggga tcacctcgtc ctcgccaccc tcctcgcgca ccggctcggg ggccccggtg
     5341 gtgcggttgg cgaggatgcg gtcgatcgag gcggcgagtc gctcggcgcg cagcggcttg
     5401 agcaggtagt ccaccgcgcc cacctcgtag gcgtcgacgg cgcggtcgtc gtgggcggtg
     5461 acgaagacga ccgacggggg gacggccatc gacgagaaca cacgcgccag ctccaggccg
     5521 tcgagcccgg gcatgcggat gtcgaggaag acgacctcga catccgtgcg ctccgccacg
     5581 cgagtgccgg tcatcgggtt ggcgagacca cgactcacct ggcccgctgt cgcggcctcg
     5641 cgcacgcctt ggcggctgtt gaggatgcgc agggcgtcgg tggcatcgga ggccttgagc
     5701 acgatcccga cgcgggagtc ttcacccaac aggtaggcga gttcttcgag ggcgggctcc
     5761 tcgtcgtcca ccgcgagaac gaccaggccg ccggagctgt cgttgctgtc cacgatctcc
     5821 tcatcctgcg tgtccgggac tcgcgccggg tccgggacga ccacgccccg gctccgccga
     5881 ccatggtgac ggcgaatccg tgtgagtgcc aggggccagc accaatcggg atcgaccggt
     5941 cacaccgcgc tacagaccgt accgtgccca tgtggcgcgt atctcggccg cctgtgtgat
     6001 tcctagcagg tgatcggcac cgagcgtggc tcctgccgct gcgggtacgg cgccaaggcc
     6061 cagccgggcc aacagcggac gtcgtccctc caccggcacc agctcagcct cggggaagcg
     6121 cttgccgagc actccccgag cagtacccag gccgtcgacg agtccgatct cgaccgcccg
     6181 cgcccctgtc cacacctcgc cggtgaacgg ctcggctgcc gtcttgaggc ggtcgccgcg
     6241 ccgctccagt acccattgct tgaacatctc gtgcagttcg tgctgcaggt cgcgaagcca
     6301 gtcgacgtcc tcctgcttct cagggaggaa cgggtcgagc cgagctttgg aggtgcctgc
     6361 tgtgtagagc ctgcgctgca ccccgaaccg ctcgatcagc ccgtgcagtc cgaagccctg
     6421 gctgaccacc ccgatcgatc cgacgatcga cgtggcgtgg gcgtagatct cgtcggcggc
     6481 acacgccagc cagtacccac cggatgccgc gacgtcctca cagaaggcga gcaccggcac
     6541 gcccttctcc tcggcgaggc cgcgcacccg gtccgccacg agggccgact gcgtcggcga
     6601 gccgcccggc gagttgacca gcagcgcaac ggcggccagc ccgtccggtg cgaacgcccg
     6661 ctcgagggtc ttctcgaccg cgttggcatt gatcaccgag cgagggaccg gacccgtggt
     6721 cggcgtgatc acgccgtgca gccggaccat cgacaccacg ggccggtcgg tccggtctcc
     6781 gaggcgccca ggaagcctgg aggtcagccg gtcggggagg cggagagcgt ccataccgtc
     6841 accgtacgcg ggtgagccat gactcgctga caagatcgaa gcctggtggg ctgcgggaac
     6901 gcagtcagac ccggataccc gcgcggaact tcggcaccct gagggtgatc ttcataccgg
     6961 cgccgggagc ggtgtccacc actaggccga aatcgtcgcc gaacgtggag cgcatccggt
     7021 cgtcgacgtt gccgaggccg acgtgcgcgc cggactggtg tgcgtcgtcg aggtcgtcgg
     7081 tgagccgggc ggggtccatg ccgatcccgt cgtcctccac gatgatcacg cattcggcgc
     7141 ctgcgttctc cgccgtgatc gtgatggtgc cgccgttggg cttgctggcc agcccgtgcc
     7201 gcacggcgtt ctccaccagc ggctgcaggg cgaggaacgg cagcaccacc gagagcacct
     7261 cgggcgcgat ctggagcttg atgttcagcc ggttgccgaa gcgggccttc tccagccgga
     7321 tgtagcgctc gatgttggtg agctcatccg acagcgtcgt gtactcccca gcggtgcgga
     7381 acgagtaccg ggtgaagtcg gcgaactcga tgagcagctc gcgggcgcgc accggatcgg
     7441 tgcggatgaa ggacgcgatc gtggtgagcg cgttgtagac gaagtgcggc gagatctgcg
     7501 ctcgcagcgc gcgtacctcg gcgcggttga gccgggcacg cgactcgtcg agctcggcga
     7561 gctccagctg gctcgacacg tagcgcgcga cctcggccgt ggcccgcagc agcaccggac
     7621 ccgcggtgga ggtggtcagc gcagcaagcg tgccgacgat cgcgccgtcg gtctccagcg
     7681 gcacgacaac gatgccccgc acgacgcagt cggggtggtc gcagatgatc gccgtgtgcg
     7741 acagcacctg ctggcgcccg gtggagatca cctggtcggc gagtgcccgc acgtcgggtt
     7801 cgtgctggtc ggcgtcgccg tcccaggcga gcgtctcgcc gcggttgtcg gtcatcgtca
     7861 aggcacaggt gtcgagcagc tggcgcaggt agggcagcgc gaacgcggcc gagctgtgcg
     7921 agaggccctc gcgcagcgac ggggcggcca gcgcgacggt gtgcagggtg gcgaaggtgg
     7981 cccgttccag cggcgtggcg aacgtgttgc gccggttgcg caggatgagg accacgacca
     8041 gcacgatcat gaagacgatg acagcgccgg cgatgagcag cgccgtggag ctgccgagca
     8101 gctcagccac gttccaccta cctcaccagc cgggacagcc gccggtcggc gaggggcttg
     8161 ccaccggtct ggcagcgggg acagtactgg aacgacttgc tggcgaaaga cacttcccgc
     8221 acggtgtctc cgcacaccgg gcacggcatc cccgtgcggg cgtgaacgcg cagcccagag
     8281 cgcttctcgc ccttgagctg cgccgcgccc tgcccgaccg agcgctcgac cgcatccgcc
     8341 agcacggagc acattgccgc gtacagctcg tcgatctgct caggcttcag ccgcccggac
     8401 gcggcgtagg gcgagagctt cgcggtgtgc aggatctcgt cgctgtaggc gttcccgatg
     8461 cccgcgatcg tctgctgctc gacgagcagt gtcttgagcc gctcgctgcg cccggcgagc
     8521 accgcggcga actcgtcgcg ggagagcgtg agtgcgtccg gaccgagccg cgcgataccg
     8581 ggcacctcct gcgggtcgtg cacgaggtac accgacagcc gcttctgcgt gccggcctcc
     8641 gtgaggtcga accccggccc accaacggca tcgaggtgca cgcggagctc cagcggtccg
     8701 cgacctggtt tgggtggggt ggccgaggcc gaatcgtgcc agcggagcca gccggcccgg
     8761 gacaggtgcg tgaccagatg gagcgggtcg tcggggcggt ccatgaactc gacggcgagg
     8821 aacttcccat agcgggccgc acccgtgacg acgcgccccg gcagcacgga caccggcggg
     8881 tcgaacgtct tgacggcgct catgctcgcc acgtccacgc gagcgaccgg gcggtagagc
     8941 gcgtgctcgc gcagatggtg ggcaagtgcc tcgacctcgg gcagctcggg catcgggtac
     9001 tccggacgcc tagttcaccg gctgcagtgg gctgtcagcg tgccccggcg tgccttgtgt
     9061 gcgaagccgg cgaaccacga tccgaagctc ctccttcgac tttgccccac ctcgcaccag
     9121 gcccgtccac tgctcggccg gcaggtcgta gttgccggcc gtctcggcga cgacggtcca
     9181 cggcctgcgc aggtaccagc gcacggggaa gaacagcacc accacggcgc cgagcagcca
     9241 caggaaccac gggatgtgca cgtccgcggg tgcccatacg atgacgatca gccagaagaa
     9301 gaacagggcc gacaggatga gcaccgccgc gccgcgcccg ccgtcgacgt cgtgctcgaa
     9361 gtcgtccccc gtggccggaa gcgaccactc gatgttgcga cggaccaacc acatgcgccc
     9421 gtcgacacca gccaccgtct tactccccat gatccccgcc gtcgtgagtc gggcccgtgc
     9481 accgtccact ttcccacaac ttcgagccga ggtgggtgga tcagcaggcg ggcaggacgg
     9541 cgctccggac cggggcgccg ggatctagca gataggtgac caccagctcg gcgggcaccg
     9601 cgccgtcgtt gcgcgcgagg tgccgctggc cgtcggcgaa gaacacggcg tcgcccctcg
     9661 agtaccgggt gggggcgcag tcgtcctccc ggagcaccgt gacctgaccg gattgcacga
     9721 tcgacagctc ggtgcccgga tgccggtgcc attccgtgct ttcaccgggc tggaggacga
     9781 cggtgcggac ggagtacgag gccgggcccg tggtgcgcat ggtcacccgc tcctcgacac
     9841 gtccggtggc caccaggacg gggccggcgg gcggggccgg ggcaggagca ggcggcggtg
     9901 gggggagcgg tgtcggaacg ggctcgggcg ccgagcccag ctggcccggt tgcgcgcagc
     9961 cggtcagcag gagggcggcc agccccattg cggccgcgag acgggggcct gttctgctca
    10021 cggcgcggag ggtagcgatc gtgtcgtgaa atgccgcagc gctaccagcg cagggggagg
    10081 ctggtgatcc cgttgatgaa gttggacacc agccgccgcg gctcgccgtc gagctcgacg
    10141 ggtgacagcc gcaggaactc ggtgaagaac acgcgcatct gcagccgggc gaaggtggcg
    10201 ccgagacaca ggtgcgggcc ctggccgaac gcgaggtgct cgttcgggtc gcgctcgatg
    10261 tcgaagcggt acgggtcggg gaagtggcgc tcgtcgtggt tggccgatat gtggtagacg
    10321 accaccttgt cgcccgccgc tatgcgccgt cccccgagct ctcgctcgcg cgtggcggtg
    10381 cgccggaacg tcagcaccgg cgggtgcacc cgcaacatct cctcgatcgc cgacggcaac
    10441 agcgcggggt cggcccgcag cctgcgatag gcgtcggggt tctgcacgag tgcgagtacg
    10501 ccacccggca gggcgctgcg caccgtgtcg ttcccggcga ccaccaggag gaagaagaac
    10561 atcttcagct ccgcgtcggt gagccgctca ccgtcgacct cggcctccac cagcgccgtc
    10621 atcacatcgt cggccggttc ccggcgcttg tggtcggcca gggcctccgc atagccgaac
    10681 atgtcgccga gcatcgccgg ggaacgcggg ttgaccggcc gtccctcggc gtccgtgacg
    10741 atctgcccgt gctcgggatc ctggtacccg atcacgcggt tggtccactc caggagcagc
    10801 tcccggtcgg ccgcgggcac gccgagcagg tcggcgaggt tctgcagcgg gaggtcgtcg
    10861 gtgacgtcgc ggggcaggtc gcaccggcgc tcgccggcca ccccggccag cagtgccccg
    10921 gcccgctcct ggatcacctg ggtgaaccgt tcgagcctgc gccgagtgaa ggcaccggtg
    10981 acgagccggc gcaggcgcac ctgctccggc gggtccatgt tgaggatcat ccggcggatg
    11041 aacggcagat ccgcagggtc ggggtcacgg atctgggtgg cgccgagtga cgaggagaag
    11101 tccgcaggcg agcgcaggac gtgccggacg tcgtcgtagc gggtgacggc ccagaacccc
    11161 ggtcccgcag gccagatgcc cacctcgggc tcgtcctgcc agctcacggg atccgtgtcg
    11221 cgcaacagcc ggagatcgtc ctgcggcacc ccccgcgcga aggtgcgcgg gtcgaacacg
    11281 tcgagcatct tgtgtggata ccgcgcccca tctcgtgatc gaggccgtgg tgggcggaat
    11341 cacgccatct ggggtcatat cgagttgccg atcgccctcc acccgaccat gcttggccca
    11401 agcaagttgt tgcttgctac aaccaacgtt ggtaaagaca gagtaatggg ataggcgagg
    11461 gggaagcgcg gtgggcgagg atgatctcgt ggtcgccgac cgcatcgcgc aggagctcgg
    11521 acagctgacc cggatggtca cctccctgcg ccgccaccgg ggtgacatcg ccgtcgccca
    11581 cgtcctcgtc caactggcgg agcagggacc actgcgggtg agcgccatcg ccgacgccgt
    11641 gggcagcgac ccctcgacgg tgagccgcaa ggccaccgag ctcgttgccg ccggcctcgt
    11701 cgagcgccgc ccggatcccg gcgacggaag agcacaccta ctcgccgtca ccgaggcggg
    11761 cgcacggtgc tgcgaggacg ggcgacgcaa gcgcatcgag ctcgtctccg cggtgctgtc
    11821 cggctggtcg gacgagtccc ggcagcgcct ggccgagctg ctcggccgct tcgccgacga
    11881 cgtacaggaa ttggaccggc gggtctcccg ccggtcagga ggagagaact gatgtccacc
    11941 caagtcgccg tcgagcccat caccagggcc gaaccgccac cgtcgggtga gctgacccac
    12001 cgccaggtgc tgaccatcct ggtgggcctc gccctcggga tgttcctggc cgcactcgac
    12061 cagacggtag tctcgacagc gatccgcacc atcgccgacg acctgggcgg gctcagcctc
    12121 caggcgtggg ccaccaccgc gttcctcatc acggggacga tcaccacccc gctctacggc
    12181 aagctgtcgg acatctacgg ccgcaagccg ctgttcctga ccgccatctc gatcttcatc
    12241 gccggctcgg tgatgtgcac cttcgcccag tcgatgtacg aactcgcggc gttccgtgca
    12301 gtgcagggcc tcggcgcggg cggactgttc tcgctggcgc tgacaatcct cggcgacatc
    12361 gtgccgccgc gccaacgcgc ccgctaccag ggctacatcc tcgcggtctt cggcacctcg
    12421 agcgtgctcg ggccggtgat cggcggagtg ctgtcgggcc aggccaccat cgcgggcatc
    12481 gcaggctggc gctggatctt cctggtgaac gtgccgatcg gcgccatcgc gctgatcgtc
    12541 gtcgcgcggg ttctcaccat gccgcacatc ccgcgaccac accggatcga ctggccgggc
    12601 gccctcatgc tcgcagtctt cctcgtgccg ctgctcgtcg tggcagagca gggccaggag
    12661 tggggctggg gctccacgag ctcgatcgtc tgctacgtga tcggcgtcgt cgggctcatc
    12721 ctgttcctga tcgccgaacg gtcctacggc gatgatgcaa tgctgccgct gcgactgttc
    12781 cgcaccagtg tcttcgcact gtcggcgggt gccggcatcc tgatcggcgc gggaatgttc
    12841 ggcggcatct cgttgctgcc gcagttcctg cagatcgtgc acggtgcctc tccgacggag
    12901 gcgggcttcc tcacactgcc gctcgtggcg gggatcatgg tctcgtccat cgtctccgga
    12961 cagatcacct cgcgcaccgg tcgctacaag atcttcccga tcatcggcac cgccctgatg
    13021 gtcggcgcga tgctgctcat gtacttccgc gtcgccgtag acatcccgct gtgggagctc
    13081 gacctctaca tggcgatgtt cggtctgggg ctgggtctct cgaaccagac cctcgtgctc
    13141 gccgtgcaga acgcggtgcc ggcgcgggac atgggcgtgg ccacggcgtc caccacgttc
    13201 ttccggcagt tgggcggcac cgtcggagtc gcggccttcc tgtcgatcgt gttcaacacg
    13261 gtcaccggca acatctccga cgagttcagc aaggctgcca cgacgccgga gttccaggca
    13321 gcgctggcag acccggccgt gcgcgccaac ccggccaacc agcccgtgct ggccgcactc
    13381 accggtggcg ggaacagtgg cgcgtccagc gtcctgacca actcctcgtt cctgcagacc
    13441 atcgacgcgc ggctggcccg gccgttcctc gccggcttcg ccgactcgat gaccatgacg
    13501 ttcctcatcg tcgcgtgcgt gctggccgtc gccttcgtga tcgtgctgtt cgtcaaggag
    13561 ctcccgctgc gcacgatgtc cggtgcgcag gccagggcga tggaggatgc tgcgatggca
    13621 gaggccggac cggcgggatc gacccttttc gcggaggcgg ccgtggccgg ggccgcgagc
    13681 tccgccaccc cgaacggggt cggcgggccc ggccccaacg gggcggggcg ccacgcgctg
    13741 ttgacacacg aggcactgcc ggccgctgtc ggcagcaacg gttatggcaa cggccacgcg
    13801 gtgggcaatg gaaagcctgc tccgagacct tctccgacac cgcgccacga cccgggagcg
    13861 gtcacgtacc cggccacgta cccggccacg atgcttccgg aaggggcgca tgccgactac
    13921 aacggccccg cggtgtacgg cacggtcacc cggagtgacg gcgtcccgtt ggcgcaggtc
    13981 gtcatcaccg tcgccgaccc cgcaggccgg caggaggcgc gcacggccgc agggccggac
    14041 gggtactacc gcgtcgacct ccgcaacggc ggcacctatc tagtggtcgc ggcgggtggc
    14101 acgtatcagc cgcatgccgc gatggtcgcc gtcggcgacg caccggcccg ccacgacgtc
    14161 atgctgagcg gcacgagctc ggtgatcggc acagtgctca cgcccgacgc ggccgcgggc
    14221 agccgcacgg tgggtggagc agcggtcacc ctgatcgaca tgcgcggtga cgtcgccgcg
    14281 tcgcgagtga ccgacgccga cggccgctac cagctggtag gagtgcccga cggcagctac
    14341 acgttgacgg ccgcgagccc tggctaccag cccatcgcag tgagcgtgcg gctcgacgtc
    14401 ggagcgacgg tcgagtgcga cctcgagctc ccgcagcgct cacagctgct cggcatggtc
    14461 actgcggtga gcaacggccg gggcgtgccc gaggccatgg cgacgctggt cgacgcaacc
    14521 ggcaccgtcg tcggctcgac ggtcacccgc cccgacggct cgttcgtgtt cgacgacctc
    14581 gccgagggca cgtacacgct caccgccagc gggtacgcac cggtggcgca gctggtgcac
    14641 gtcacggcgg gagcgcaggc ctccaccgtg gtggagctcg gcacgcctgc cccggccgaa
    14701 gcaccgagta cggcaccccc cgcagagcag cagttcgacg tgttcgagcc gttcgacgca
    14761 gggggtgtgc gatgagcgag cctgcgagcg aatcaacggc acagcgcctc cgcgaagcgc
    14821 cgaccgagcg cagcgaggga gtgcaatgag ccaggtgagc ggtcagctga cgacgcagga
    14881 cggttggccg gtggcgggcg ggaccctcac ggccgtggac gcgggtggcg tgcaacgtgc
    14941 ccgtgcggcc agcggcacgg acggtcgctt cgtcctcg
//
LOCUS       DASRSC010000113         8287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3148504,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000113 DASRSC010000000
VERSION     DASRSC010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8287
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3148504"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFE39_05370"
     CDS             complement(<1..678)
                     /locus_tag="VFE39_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019513359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6258065.1"
                     /translation="MDHAPDDTDADGLAALRHHGDVEAEAGLLDFAVNVQGDGPPVWL
                     RERLAATLDGLGRYPAATHDARARDAVAARHRRRPEEVLVLAGSAEGFALLPALRPRL
                     AVVLHPGFTEPEAVLRSAGIPVVRVLTSESDGHRLHPGAIPAEADLVVVGNPTNPTAV
                     LHPAADVRARAAPGRVLLVDEAFADALPGEPESLAGAAGVPGLLVFRSLTKTWALAGL
                     RAGYALGE"
     gene            complement(752..1069)
                     /locus_tag="VFE39_05375"
     CDS             complement(752..1069)
                     /locus_tag="VFE39_05375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258066.1"
                     /translation="MRDPGINAAVNAAMGAIRLIVEEREDAAQGAGNNVVAVIRELAD
                     KHGPRAVEITALFLAQAAASGLQAIARIEEDSVENVVARYQQHFETRVDFTLLTAQLR
                     DEE"
     gene            1261..1719
                     /locus_tag="VFE39_05380"
     CDS             1261..1719
                     /locus_tag="VFE39_05380"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HET6258067.1"
                     /translation="MHVVFVCTGNICRSPIAEKVFVQELERAGLADAVHVSSAGTGGW
                     HIGSPADERAAALLRAEGYPDAHTARQVDAEQLSADLLVALDNSHRRALQSMVPDPER
                     VRLLRSFDPAAPPRAEVPDPYYGTADGFAEVLAMIRAAMPGLVDWVRSRR"
     gene            1716..2543
                     /locus_tag="VFE39_05385"
     CDS             1716..2543
                     /locus_tag="VFE39_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HET6258068.1"
                     /translation="MTVLGVAVRATTPLGGGAVRVDLADGRTVVVKGAEATAVAAEAA
                     GLRWLGVPGGPPVPTVLDVDQERLVTEFVPPGAPDAAAAAALGSRLARMHAAGAGAFG
                     AAPPDGPAAAWIGRAPMRNEPSPSWPEWYAADRVLPYLRAASDAGDLTGREAAIVEVV
                     CDRLRALAGPAEPPARLHGDLWSGNVLWSRGGAWLIDPAAHGGHRETDLAMLALFGCP
                     HLDAVLDAYDETAPLADGWRERVPLHQLFPLLVHVVLFGRGYARQAVAAARAALRDG"
     gene            complement(2611..3537)
                     /locus_tag="VFE39_05390"
     CDS             complement(2611..3537)
                     /locus_tag="VFE39_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6258069.1"
                     /translation="MTIEVRPLAEADVDAADHIFRDAFGAFLGVDMYDDTDLFRTRFR
                     APHTAALAAFVDGEIVGSVLVTLWGSVGYLGPLSVTPDRWGAGVGGRLVDAAMDILGG
                     RGVTHQGLFTFAQSPKHHWLYQKFDLWPRFLTVIMSRPVTGGEPVLGWRLSAAAPAAR
                     RALVDACDAVTGAILPGLDVRGEIAAVLDQRLGEVVLIGEERTPRGFAICHTGPGTEA
                     GSGIAYVKFAAVRPGPHVADAFGALLDACQGFAAGAGARRLTLGVNTARHGAYRRLLA
                     AGFRVDTAGVTMHRPNEPGYDREDIYLLDDWR"
     gene            complement(3686..5077)
                     /locus_tag="VFE39_05395"
     CDS             complement(3686..5077)
                     /locus_tag="VFE39_05395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HET6258070.1"
                     /translation="MPDRLSPLDASFLFAEHRTAAMHVGAVMTFDPPEHGRFDAEAFT
                     DLIGSRLALVPRYRQKVRDVPVRLGLPVWVDDPDFDLGFHVRRSALPAPGTDDVLRDL
                     VGRLLARQLDRSRPLWEVYLVEGLADGRFAVVTKTHHAMVDGLASMDIGGALLDLTPE
                     PRETPPDDWRPAPEPSGLELAAMAAAHSLRRPRAALDVAGRAVADVREAASVVGRTVE
                     AVLAASRSAAATRPVQPLNAATGEQRRYGMERTSLADHRAVRKAHGGTVNDVVLAVVA
                     GGLRRWLISRGEPLTADTAVRAMVPVSVRARTRAEGSGNSISAYLVHLPVAEADPLVR
                     LAAVRDAMEIHKRGGQAVGAAALVGLVGLAPPIVHSLGARLASQFSSRLYNVLVTNVP
                     GPPRPLYAMGARMRDMYPVVPLAGGQAVAIGVTSYDGGVHYGLTADRDALPDVDVLAG
                     AMSESLAELVAHS"
     gene            complement(5158..6390)
                     /locus_tag="VFE39_05400"
     CDS             complement(5158..6390)
                     /locus_tag="VFE39_05400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HET6258071.1"
                     /translation="MQVADIISGVVGPDVPVRVIGYDGSKAGPDSSEMALRITSPRAL
                     ARLATAPGTLGLARAYVEGEIEVEGDVYELLDAMADLTLNDSITRADQLRLAQRLLPI
                     WLRHRRPPPDLEFRPPGRLHSKARDRKVISHHYDVSNRFYEWVLGPSMAYTCAVYPTD
                     TATLEEAQAAKHELVAQKLGLEPGMRLLDVGCGWGGMVMHAAAEHGVKALGVTLSRNQ
                     AEWAQAEITRRGLGGLAEVRHLDYRDAPESEFDAISSIGLTEHIGKAQLPNYFSSLYA
                     RLRPGGRLLNHCITQPRTPTKRRLDPFIARYVFPDGQLEPVGHLVSVMNDVGFEIRHE
                     ENLREHYASTLAGWGANLEAHWDEAVAEVGIGRARVWRLYMAACRLGFDRDNIQLHQV
                     LGVKLLDQGRSGFPLRGW"
     gene            complement(6407..7792)
                     /locus_tag="VFE39_05405"
     CDS             complement(6407..7792)
                     /locus_tag="VFE39_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6258072.1"
                     /translation="MSAGAHMSTPPVVSPSVHAAAVADLVGRYRALPPGTRVRLGKKS
                     SNLFRFGGRADDTARLDTAALTGVIGVDPVARTAEVQGMTTYEDLVAGTLAHGLMPLV
                     VPQLKTITLGGAVTGLGIESTSFRHGLPHESVLEMDVLTPDGEIVTATPDGEHAALFQ
                     GFPNSYGTLGYALRLKIELQPAAPYVRLEHRRVASSDLAARITELCDLDAVGGPDFVD
                     GTVFTAGEQYLTIGTFTDDPGPGPSDYTGQQIFYRSIRERTVDHLTAHDYIWRWDTDW
                     FWCSRAFGVQNPLVRRLWPRQYRRSDVYRRLVALDRRTGFSSRIHRLTRTPQEEPVIQ
                     DVEIPVERLAEFLDAFHTDIGIAPVWLCPLRLRGKQRWPLYPMEPGELYVNAGFWSAV
                     PEVPGQPEAHNRRIEELVASLGGHKSLYSTVHYGEGEFWDHYNGPAYRALKERYDPHG
                     KLPDLYSKVSG"
     gene            8059..>8287
                     /locus_tag="VFE39_05410"
     CDS             8059..>8287
                     /locus_tag="VFE39_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6258073.1"
                     /translation="MQRDIFDAEHNAFRDMARTFIAKEITPFHDRWERDGQVSRDVWV
                     AAGKAGLLGTDVPEEYGGGGVADFRYNAVLLE"
BASE COUNT         1155 a         2963 c         3000 g         1169 t
ORIGIN      
        1 ctcccccagc gcgtacccgg cgcgcagccc cgccagcgcc caggtcttgg tgaggctgcg
       61 gaacacgagc aggccgggca cgccggcggc accggcgaga gactccggct cccccggtaa
      121 ggcgtcggcg aacgcctcgt cgaccagcag cacccggccg ggtgccgccc gcgcgcggac
      181 gtcggccgcg gggtgcagca cggccgtcgg gttggtgggg ttgccgacga ccacgagatc
      241 ggcttccgcc ggaatggcgc cgggatgcag ccggtgaccg tcggactcac tggtcagaac
      301 ccgcaccacc ggtatgcctg ccgaccggag cacggcttcg ggctcggtga aacctgggtg
      361 cagcacgacg gcgagccgcg ggcgcagcgc cggcagcagc gcgaacccct ccgcgctgcc
      421 ggcgagtacc aacacttcct cgggtcggcg cctgtgccgg gcggcgacgg cgtcccgagc
      481 gcgggcgtcg tgcgtggcag ccggatagcg gcccagcccg tccagcgtcg cggcgagccg
      541 ctcgcgcaac cacaccggcg gcccgtcacc ctggacgttc accgcgaagt cgagcaggcc
      601 ggcttcggcc tccacgtcgc cgtggtgacg cagggccgcc agaccgtctg cgtccgtgtc
      661 gtccggcgca tgatccatca cagcagccta cggtggacgc cgtgcaggtc gtcttcgtcc
      721 gtaccggcaa ctcagcgacc gtcaccgtcg ttcactcctc gtcgcggagc tgagccgtga
      781 ggagggtgaa gtcgacgcgg gtctcgaagt gctgctggta gcgggccacg acgttctcga
      841 ccgagtcctc ttcgatacgc gcgatggctt gcaagcccga ggcggcggcc tgggcgagga
      901 acagcgcggt gatctcgact gcgcggggac cgtgcttgtc cgccagttct cggatgactg
      961 cgacgacgtt gttaccggcg ccttgagctg cgtcctcgcg ctcttcaacg atgagtcgga
     1021 ttgcgcccat ggcggcgttg acggcggcgt tgataccggg gtctctcacc cgctcagggt
     1081 cgcagctcgg ggtccctgac gagaggactc ggacgtcttg tcgccccggc agcggccggt
     1141 cacgccaacc cgagcagccc ggcttcggcc tcgacatcgc cgtggtgacg cagggccacc
     1201 agagtgtctg cctccgtgtc gtccggcgca tgatccatcc cggccgccta cggtggacgc
     1261 gtgcacgtcg tcttcgtctg taccggcaac atctgccgct cccccatcgc cgagaaggtg
     1321 ttcgtccagg agctggagcg cgctgggctg gccgacgcgg tgcacgtcag cagcgcgggc
     1381 accggcggct ggcacatcgg ctcacccgcg gacgagcggg ccgcggcgct gctgcgcgcc
     1441 gagggctacc ccgacgccca cacggcgcgg caggtcgacg ccgagcagct gtcagccgat
     1501 ctgctcgtcg ccctcgacaa ctcgcaccgc cgtgcgttgc agtcgatggt ccccgatccc
     1561 gaacgggtgc ggctgttgcg ctccttcgac cccgctgccc caccgcgtgc cgaggtgccc
     1621 gacccgtact acggcaccgc cgacgggttc gccgaggtgc tcgcgatgat ccgggcggcg
     1681 atgccaggac tcgtggactg ggtgcgcagc aggcgatgac ggtcctcggc gtggcggtcc
     1741 gtgccaccac gccgctcggc ggcggcgccg tccgagtgga tctcgccgac gggcgcaccg
     1801 tcgtcgtcaa aggcgctgag gcaacggccg tggccgccga ggccgccggc ctgcgttggc
     1861 tgggggtgcc cggcggcccg ccggtgccaa cagtgctgga cgtcgaccag gagcggctgg
     1921 tcacggagtt cgtcccgccc ggtgcacccg acgccgcggc cgcggcggcc ctgggcagcc
     1981 ggttggcgcg gatgcacgcc gcaggcgcag gggcgttcgg tgccgctccg ccggacggac
     2041 cggcggccgc ctggatcggg cgggccccga tgcggaacga gccctccccg agctggcccg
     2101 agtggtatgc cgccgaccgc gtgctgccct acctacgcgc cgcgtccgac gccggggatc
     2161 tgaccggccg ggaggccgcc atcgtcgagg tcgtgtgcga ccggctccgc gcgctggccg
     2221 gaccggccga gccaccggcc cggctgcacg gggacctgtg gtcgggcaac gtcctgtggt
     2281 cccgcggcgg cgcatggttg atcgacccgg ccgcgcacgg cgggcaccgt gagaccgacc
     2341 tcgcgatgct cgcgctgttc gggtgtccgc acctcgacgc cgtgctggac gcgtacgacg
     2401 agacggcacc gcttgccgac ggatggcgcg agcgcgtgcc cctgcaccag ctcttcccgc
     2461 tgctggtgca cgtcgtgctg tttgggcggg gctacgcccg gcaggccgtg gcggcggcgc
     2521 gggcggcgct gcgggacggc tgatgcggca cgactcgcgg gcggaaaacg ggctactcgc
     2581 gggctgggaa tgggcgactc gcggggtggg tcagcgccag tcgtcgagga ggtagatgtc
     2641 ctcgcggtcg tagccgggct cgttcgggcg gtgcatcgtg acgcccgcgg tgtccacgcg
     2701 gaagccggcg gccaggaggc ggcggtacgc gccgtggcgg gcggtgttca cgccgagggt
     2761 gagccgtcgg gcacctgccc ccgcggcaaa gccctggcag gcgtcgagca gtgccccgaa
     2821 cgcgtcggcc acgtgcgggc cgggccgcac ggccgcgaac ttcacgtagg cgatcccgct
     2881 gcccgcctcc gtgccgggcc ccgtgtggca gatcgcgaac ccgcggggag tgcgttcctc
     2941 accgatgagc acgacctcac cgaggcgctg gtcgagcacc gcggcgatct cgccgcgtac
     3001 gtcgaggccc ggcaggatcg cgccggtcac ggcgtcgcag gcgtccacca acgcccgccg
     3061 ggcagcaggg gccgcggcgg acagccgcca ccccagcacg ggctccccgc cggtcaccgg
     3121 ccgcgacatg atcacggtga ggaaccgggg ccagagatcg aacttctggt agagccagtg
     3181 gtgcttgggg ctctgggcga aggtgaacag tccctggtgc gtcacccccc ggccgccgag
     3241 gatgtccatg gccgcatcga caaggcgccc gccgacccct gcaccccacc gatccggcgt
     3301 caccgacagc ggcccgaggt acccgacgct gccccacagc gtcaccagga cggagccgac
     3361 gatctcaccg tcgacgaacg cggcgagcgc ggcggtgtgc ggcgcgcgga agcgcgtgcg
     3421 gaacagatcc gtgtcgtcat acatgtcgac gccgaggaag gcgccgaacg cgtcgcggaa
     3481 gatgtggtct gccgcgtcca catcggcctc ggcgagcggc cgaacctcaa tcgtcacggc
     3541 ggcaggatgc cgcgtcgcta ctcctaccgg gccaccgggt tcccccgaag gcgcaggact
     3601 gcgtcagcct cgctccacgc tcagtaccgg ccgcctcggc caccctcgcc ggtgcggtgt
     3661 tgctgcgcgc gtccagacag cggcgtcagg agtgcgccac cagctcggcc agcgactcgc
     3721 tcatggcacc ggcgagcacg tcgacgtcgg gcagcgcgtc gcggtcggcg gtgaggccgt
     3781 agtgcacgcc gccgtcgtag gaggtgacgc cgatggcgac ggcctgcccg ccggcgagcg
     3841 gcaccaccgg gtacatgtcg cgcatccggg cgcccatcgc gtacagcggg cgcggcggac
     3901 cgggcacgtt ggtgaccagc acgttgtaga ggcggctgga gaactggctg gccagccgtg
     3961 caccgaggct atggacgatc ggcggggcga gcccgacgag cccgaccagc gcggccgccc
     4021 cgaccgcctg accgccgcgc ttgtggatct ccatcgcgtc gcgcacggcc gcgagccgca
     4081 ccagcgggtc cgcctcggcg acagggaggt gaacgaggta cgcggagatg ctgttgccgg
     4141 agccctctgc gcgcgtcctg gcccggacgc tcaccggcac catcgcccgc acggcggtgt
     4201 cggcggtgag cggctcaccg cggctgatca gccagcggcg gagcccgccg gcgacgaccg
     4261 cgagcaccac gtcgttgacg gtgccgccgt gcgcctttcg gaccgcccgg tggtcggcca
     4321 gcgaggtgcg ctccatgccg tagcggcgct gttcaccggt agcggcgttc aacggctgca
     4381 ccgggcgcgt cgccgctgcg ctgcggctgg ctgcgagcac cgcctcgacc gtccgcccca
     4441 cgaccgacgc ggcctcccgc acgtcggcga ccgcccggcc cgcgacgtcg agcgcggccc
     4501 gcgggcggcg cagcgagtgc gcggcggcca tcgccgcgag ctccagcccc gagggctccg
     4561 gcgccggccg ccagtcgtcg ggcggggtct ctcgcggctc cggggtgagg tcgagcaacg
     4621 cgcccccgat gtccatcgac gccagtccgt ccaccatcgc gtggtgtgtc ttggtgacga
     4681 ctgcgaagcg cccgtccgcc agtccctcca cgaggtagac ctcccacagc gggcgggaac
     4741 ggtcgagctg gcgggccagc agcctgccca ccagatcgcg caggacgtca tcggtgcccg
     4801 gtgcgggcag cgccgatcgc cgcacatgga atcccaggtc gaaatcgggg tcgtccaccc
     4861 acaccggcag cccaagacgt acgggtacgt cccggacctt ctgtcggtag cgcggaacga
     4921 gcgcgagcct gctgccgatg agatcggtga acgcctcggc gtcgaaccgc ccgtgctccg
     4981 gcggatcgaa ggtcatcacc gcgccgacgt gcatcgctgc cgtgcgatgc tccgcgaaga
     5041 ggaacgatgc gtcgagcggt gagagacggt ccggcacgtt gttcattgtc ccgacctgca
     5101 cgatcgtgtc cggcccgggt gcttcggcac ccgtgtccgc acacggacgg cggagcgcta
     5161 ccacccccgc agggggaagc cggaccgtcc ctggtcgagc agcttgaccc ccagcacctg
     5221 gtgcagctgg atgttgtcgc gatcgaaacc gagccggcat gcagccatgt agagccgcca
     5281 caccctcgcc ctgccgatcc cgacctcggc gaccgcctcg tcccagtgcg cctccaggtt
     5341 ggcgccccac ccggccagcg tgctggcgta gtgctcgcgc aggttctcct cgtgccggat
     5401 ctcgaagccg acgtcgttca tcacgctgac gaggtgcccg accggctcga gctgcccgtc
     5461 ggggaagacg tagcgggcga tgaaggggtc gagccgccgc ttcgtggggg tacgcggctg
     5521 ggtgatgcag tggttgagca gccggccgcc gggacggagc cgggcgtaga gcgacgagaa
     5581 gtagttcggc agctgtgcct tgccgatgtg ctcggtgagc ccgatcgagc tgatcgcgtc
     5641 gaactccgac tccggcgcgt cgcggtagtc gaggtggcga acctcggcga gcccgccaag
     5701 cccgcgccgg gtgatctcgg cctgtgccca ctcggcctgg ttgcgcgaca gcgtcacgcc
     5761 cagcgccttg accccgtgct cggcggcggc gtgcatcacc atgccgcccc acccgcagcc
     5821 gacgtccagc aggcgcatgc cgggctcgag ccccagcttc tgggccacca gctcgtgctt
     5881 ggctgcctga gcctcctcga gcgtcgcggt gtcggtcggg tacaccgcgc aggtgtaggc
     5941 catcgacggt ccgagcaccc actcgtagaa ccggttggac acgtcgtagt ggtgcgagat
     6001 gaccttgcgg tcgcgggcct tggagtgcag ccggcccggc gggcggaact cgaggtccgg
     6061 cggcggccgg cggtgccgca gccagatcgg caggagccgc tgggccagcc gcagctggtc
     6121 ggcccgggtg atgctgtcgt tcagcgtcag gtcggccatc gcgtcgagca gctcgtacac
     6181 atcgccttcg acctcgatct cgccctcgac atatgccctg gccaggccca gcgtccccgg
     6241 ggcggtcgcg agccgggcca gcgcgcgcgg cgacgtgatc cgcagcgcca tctccgagct
     6301 gtccggcccc gctttactgc cgtcataacc gatcactcgc acgggcacgt ccggcccgac
     6361 gaccccggag atgatgtctg cgacctgcat cccgctcacc gccccgttat ccgctcacct
     6421 tggagtaaag atcgggcagc ttcccgtgcg ggtcgtagcg ctccttgagc gcacggtagg
     6481 caggcccgtt gtagtggtcc cagaactcac cctcgccgta atgcacggtc gagtacagcg
     6541 acttgtggcc accgagggac gcgaccagtt cctcgatccg ccggttgtgc gcctccggct
     6601 gccccggtac ctccggaacc gctgaccaga agcccgcgtt gacgtacagc tcccccggct
     6661 ccatcgggta cagcggccag cgctgcttcc cgcgcaggcg cagcgggcac agccagaccg
     6721 gcgcgatccc gatgtcggtg tggaacgcgt cgaggaactc cgccagccgc tccacgggga
     6781 tctcgacgtc ctggatcacc ggctcctcct gcggcgtgcg ggtgagccgg tggatccggc
     6841 tgctgaaccc ggtgcgccgg tcgagggcga cgagcctgcg gtagacgtcg gaacggcggt
     6901 actggcgcgg ccacaaccta cgcaccaggg ggttctgcac gccgaacgcc cgcgagcacc
     6961 agaaccagtc ggtgtcccag cgccagatgt agtcgtgtgc ggtgagatgg tcgaccgtgc
     7021 gctcacggat ggagcggtag aagatctgct ggccggtgta gtcgctcggc ccgggtccgg
     7081 gatcgtcggt gaaggtgccg atcgtgaggt actgctcgcc tgcggtgaac acggtgccgt
     7141 cgacgaagtc cgggccgccc acggcgtcga ggtcgcacag ctccgtgatc cgcgcggcga
     7201 gatcggagga cgccacacgc cggtgctcca gccgaacgta gggggccgcc ggctgcagct
     7261 cgatcttcag ccgcagcgcg tagccgagag tgccgtagga gttggggaag ccctggaaga
     7321 gggcggcgtg ctccccgtcc ggagtggcgg tgacgatctc gccatcgggg gtgagcacgt
     7381 ccatctcgag caccgactcg tgcggcaggc cgtggcggaa cgacgtggac tcgatgccga
     7441 gcccggtgac cgctcccccg agggtgatcg tcttgagctg cgggacgacc agcggcatca
     7501 gcccgtgcgc gagcgtgccg gcgacgagat cctcgtaggt ggtcatgccc tgcacctcgg
     7561 cggtgcgggc caccgggtcg acaccgatca cgccggtgag ggcggctgtg tccagccgcg
     7621 ccgtgtcgtc cgcgcgtccc ccgaaccgga acaggttcga cgacttcttc cccagccgca
     7681 cccgggtgcc gggcggcagc gctcggtacc ggccgacgag atcggccacc gcggcggcat
     7741 gcaccgacgg tgagaccacc ggaggtgtgg acatgtgtgc tccggcgctc atgacgggga
     7801 cgctagttca ccatgcgatt ctgcccaacc actcagatag ggcttttccg atagggcttt
     7861 cgtcacatgg tggtcccgag ctgccggatt catccgattc gcactgtcca tcatgtctgc
     7921 gtgcagcgcg ggcgaccgtg gtctgatgtg gatcgtccct gtccgccggc ggcaccgccc
     7981 atggtcgctg cggcgtggag ccgggcggcg accccgccac aagatccggc gatcccggcg
     8041 gcgtgggaag ctcccggcat gcagcgggac atcttcgacg ccgagcacaa cgccttccgg
     8101 gacatggcgc gcacgttcat cgcgaaggag atcacgccgt tccacgaccg gtgggaacgc
     8161 gacggccagg tgagccgcga cgtgtgggtg gccgcgggca aggccgggct gctcggcaca
     8221 gacgtaccgg aggagtacgg cggcggcggg gtggcggact tccgctacaa cgccgtgctg
     8281 ttggagg
//
LOCUS       DASRSC010000114         8244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1346168,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000114 DASRSC010000000
VERSION     DASRSC010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8244
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346168"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1426)
                     /locus_tag="VFE39_05415"
                     /pseudo
     CDS             complement(1..1426)
                     /locus_tag="VFE39_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674133.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
     gene            complement(1423..4065)
                     /locus_tag="VFE39_05420"
     CDS             complement(1423..4065)
                     /locus_tag="VFE39_05420"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET6258074.1"
                     /translation="MTDTLPPRTADLPAQWNPGEVEAGMYQRWVEAGYFTADASSDKP
                     PFCIVIPPPNVTGSLHIGHAFEHTLIDILVRRRRMQGHETLWLPGMDHASIAVHALVE
                     RQLAAEGTSRRELGREAFVERTWEWKAEHGGAILAQMRRLGDSVDWSRERFTMDEGLS
                     RAVNTIFKRLYDDGLIYRAERMVNWSPELRSVLSDVEVEHREVEGELVSIRYGDGEDS
                     LVVATTRVETMLGDTAIAVHPDDERYAHLVGREIELPLVGRMLRIVADEHVDPSFGTG
                     AVKVTPAHDPNDFEIGRRHDLPMPSIMDESGRITGTGTVFDGMDRFEARVAVREALAA
                     QGRIVAEKRPYLHSVGHSSRGSKEPIEPRLSLQWFVKVGPLAQAAGDAVRDGRVTVHP
                     QELEPRWFGWVDNMHDWTISRQLWWGHRIPVWYGPDGEVVCVGPDEEPPSGAGWTQDE
                     DVLDTWFSSALWPFSTLGWPDKTPDLEKFYPTSVLVTGYDILFFWVARMMMFGLYAMQ
                     DRGPEESVPFHTIALHGMVRDQFGKKMSKSAGNTVDPLEWMDRYGTDSLRFTLARGAN
                     PGTDVPIGEDAVQASRNFGTKLWNATRFALANGASPALPLLNALTDADRWILGRLREV
                     REQTDALLEDFQFAKAVEGLYHFTWDELCDWYVELAKVQLRDDATADGTRAVLGHVFD
                     ALLRLLHPVIPFLTEALWQALTDGESLVVADWPVDAGVDTDPAAAARVLDLQKLVTEI
                     RRFRTEQRVPDSRRVAARVVGLDEAGLGGHRGAVASLVRLDDPDAEFSPTATFEVGLG
                     GGTVHVEIDTSGAIDVAAERARLARDLTVAQKELDQAEKKLGNPKFVEKAPADVVDGI
                     RSRRAAAAADIDRIGARLAALPGS"
     gene            complement(4062..4805)
                     /locus_tag="VFE39_05425"
     CDS             complement(4062..4805)
                     /locus_tag="VFE39_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258075.1"
                     /translation="MRTLFGMARRIQSLVGRGLRKQGRVAAVLPLAGGRRPLLTEPIG
                     EQRRGGARLDQVDRATCGSAVLVALSAWADPAEMLRLDGPTAGRGLGFGARYDARQRQ
                     VHRETNRFWPRALGTTPWGMVRWLRRHVPGAGPYRVRLVDDTSAADLTGALDEAGAAL
                     AAGRPVPMLVGPLVPRHYVMALGVLDGGAWRVYEPTSGQVRALDLRLVRERRLAPVLG
                     FHRLHALLLPPAGVGARVTGSIPARRLGG"
     gene            complement(4870..6429)
                     /locus_tag="VFE39_05430"
     CDS             complement(4870..6429)
                     /locus_tag="VFE39_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HET6258076.1"
                     /translation="MRSHHAVDRRTLLRTAVIGGLASAVPLGTGTSAFAAPALARPDR
                     PVLTHGVQSGDVLAGSGLVWARADRPSRMIVEVSDDPDFRRSRTFRGPVLTPDTDFTG
                     RVRVPGEGRTVHYRVTAEDLDGRSGSEPASGSFRTLPGKRDGVRFVWSGDVVGQGWGI
                     NPDLGGMRIFAAMAERDPDFFLHSGDTVYADGPLTETVTLPDGRTWRNVVTPEKLKVA
                     ETLAEFRGQYAYNLLDDNLRGFAAAVPQFNQWDDHEVLNNWYPGEMITDDRYTEKHVD
                     VLAKRAFQAFHEWVPLDPRRAVDGRVYRQFPYGPHVEVFVLDMRTYRDVNSANTAPME
                     RILGDRQARWLVDALASSKATWKIVASDMPIGVQVPDGTAWEAVANGAAGPPSGREAE
                     IAWVLKGLRDRKVRNVVWLTADVHYTSANHYSPERAGSAGGFTEFDEFWEFVSGPLHA
                     GAFGPNPLDPTFGPEAVFVKAPPAANTSPMDGFQHFGEVEVAPGGGELQVTLRGQDGG
                     ELWSRTLTAAR"
     gene            complement(6500..7702)
                     /locus_tag="VFE39_05435"
     CDS             complement(6500..7702)
                     /locus_tag="VFE39_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004562813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cmx/CmrA family chloramphenicol efflux MFS
                     transporter"
                     /protein_id="HET6258077.1"
                     /translation="MSVAAAPPGTTRIPRVVPVLALSIFALGTSEFMIAGLLPELAAD
                     LRVGIPEAGYLISAFAVGMIVGAPAMAVLTLRLPRRITLLAALGVFVVGHVIGALAQG
                     YGVLLVARVVTAVATGAFWAVAAVVTVSVVAPELRAMALSVLLGGLTVANVAGVPLGT
                     LVGQQFGWRSAFWAVAALATASAVGVLLTVPQRTAGMPRPRLAAEVAAFRRGRLWLAL
                     GTTALYQSAMIGAFSYIAPLLTDVTGLEERWVPGVLLGFGIGSLAGVVIGGRLADPYP
                     WPTLFGGLTAAAVLLGLVAVFARVPVAVVGLVVLLGAVAFIAGAPLNARVFALAGAAP
                     TLASATNTSAFNVGNSVGPAVGGLAIAAGWGYTAPAYLGVLLAAGAIAVAAVSRRLDR
                     RDALRSES"
     gene            7815..>8244
                     /locus_tag="VFE39_05440"
     CDS             7815..>8244
                     /locus_tag="VFE39_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET6258078.1"
                     /translation="MSMISPEERAFLTHGTRTGKLGFVAADGRPLVAPIWFVLEGTDD
                     APELVFNTGKGTAKGRAVARDPRLVLCVDLERPPYGFVQVQGEATISEDPDELLRTAT
                     AIAARYMGADRAEEFGRRNGVPGELVVHLRPTKVVAELHMT"
BASE COUNT         1212 a         3032 c         2905 g         1095 t
ORIGIN      
        1 cgacgacgcc cactcccgac tcgccgccct cctctgccag ctcccccgcc tgctcgacgg
       61 cctcggcgag caccggtgcg acgctcacac gaccggagcc gaacacctcg acggcgagcg
      121 caccgagctc gtcggggtcc atggcgcgcg gcgaggagtt ggccgtgatg accacctcct
      181 gcagcaccgg ttccagctcc tccagcatgc cgcgggcgtc cttctgctgc atgacgccga
      241 tgacgccgat caggcgcgcg aaccggaagt cggtggtgag cgcggtggcg agggcgcggg
      301 caccgtgcgg gttgtgcgcg gcgtcgacga gtaccgtcgg tacgccctga cctgcggcca
      361 gccgctcgag cctgcccggc gaggtgacgc ccgcgaacgc ggcccggacg gcgtcgggat
      421 cgagaggctg cttcggtccg gcgcccacca gcgcctagac ggcggccagt gcgagcgcgg
      481 cgttgcttgc ctggtgctca ccgtggagcg gcaggaagat gtcgtcgtag ttgccgctca
      541 gaccctgcag cgtgatccgc tgcccgccca cggcgatctc ccgctcccgc accccgaact
      601 cggcgccctc gcgggccacc tgggcgtcca cctcgacgca ccgctccagc agtacctcgg
      661 cgacggcctt gtcctgcgcg gccagcaccg ccacggcgcc cggcttgatg atcccggcct
      721 tctccctggc gatcccgacg acgtcgtcgc cgaggaactc cacgtggtcc accccgatcg
      781 gggtgatcac cgcgacgttg ccgtccgcca cgttggtggc gtcccagcga ccgcccagcc
      841 cgacctcgac gatcgcggcc tcgaccggcg catccgcgaa cgccgagaag cccatcgccg
      901 tcagcacctc gaacttcgac agcacgccgt gcttggcgtc gaccaggtcg atgaacggtg
      961 cgacgtcgcg gtagatctcg acgtagcgct ccggggtgat cggccggttg tcgatgctga
     1021 tccgctcggt ggcccgctgc aggtgcgggc tcgtgtagcg gcccgtgcgc aaaccgatct
     1081 cggtgagcag cgcgtcgatc atccgcgagg tcgacgtctt gccgttggtg ccggtcaggt
     1141 gcacgacggg atagccacgg tgcggctcgc cgagcacgtc gacgagcgcg gcgatccgct
     1201 ccagggacgg ttcgatcacc gtctcgggcg cccgccggtc gagctcggca tcggtggcga
     1261 ggaactccgc gaagccgatc accgacgact gcgccggtac gacgtcgggg ctctcgcccc
     1321 cgcggatcgc gtcgagcacg tgcgagacca ccgcgtcctc ggccgactcc tccggcgagc
     1381 ggaccccgcc ctcgccctcc ggcagctcct ccggatccct gctcatgagc cgggcagcgc
     1441 ggccagccgg gctccgatcc ggtcgatgtc ggcggccgcc gccgcgcgcc gggaccggat
     1501 gccgtcgacg acgtccgcgg gcgccttctc gacgaacttg gggttgccga gcttcttctc
     1561 cgcctggtcg agctccttct gcgcaacggt caggtcacgc gcaagccggg cccgctcggc
     1621 ggcgacgtcg atggcgcccg atgtgtcgat ctcgacgtgg acggtgcccc cgccgagccc
     1681 gacctcgaac gtcgccgtgg ggctgaactc cgcgtccgga tcatccagcc ggaccagcga
     1741 ggcgacggcg ccgcggtgcc cgccgagccc cgcctcgtcc agcccgacca cgcgggcggc
     1801 gacgcgacgg ctgtcgggca cgcgctgctc cgtgcggaag cggcggatct cggtgaccag
     1861 cttctgcagg tcgaggacac gagccgcggc agcggggtcg gtgtccacgc cggcgtcgac
     1921 cggccagtcc gcgacgacga gcgactcccc gtcggtcaac gcctgccaca acgcctcggt
     1981 gaggaacggg atgaccgggt gcagcaaccg caggagcgcg tcgaagacgt gaccgaggac
     2041 cgcacgggtg ccatcagccg tggcatcgtc acggagctgg accttggcca gctcgacgta
     2101 ccagtcgcac agctcgtccc acgtgaagtg gtacagaccc tcgaccgcct tggcgaactg
     2161 gaagtcctcc agcagtgcgt cggtctgctc gcggacctcg cgcagccttc cgaggatcca
     2221 gcggtcggcg tcggtgagag cattcagcag tggcagcgcc ggggacgcgc cgttcgccag
     2281 cgcgaaccgg gtggcgttcc acagcttggt gccgaagttg cgcgacgcct ggacggcgtc
     2341 ctccccgatg gggacgtcgg tgcccgggtt ggcgccgcgg gcgagggtga agcgcaggga
     2401 gtcggtgccg tagcggtcca tccactccag cgggtcgacc gtgttgcccg ccgacttcga
     2461 catcttcttg ccgaactggt cgcggaccat gccgtgcagc gcgatggtgt ggaacgggac
     2521 cgactcctcc gggccccggt cctgcatcgc gtacaggccg aacatcatca tgcgggcgac
     2581 ccagaagaac aggatgtcgt agcccgtgac aagcaccgac gtgggataga acttctcgag
     2641 gtcgggggtc ttgtccggcc agcccagggt ggagaacggc cagagggccg aggagaacca
     2701 ggtgtcgagg acgtcctcgt cctgggtcca acccgcaccg ctcggcggtt cctcgtcggg
     2761 gccgacgcag acgacctcgc cgtcggggcc gtaccagacc gggatccggt ggccccacca
     2821 cagctgccgg ctgatcgtcc agtcgtgcat gttgtcgacc cagccgaacc agcgcggctc
     2881 gagctcctgc gggtgcacgg tgacgcggcc gtcgcggacg gcgtcaccgg cggcctgcgc
     2941 gagcgggccg accttgacga accactgcag cgacagccgc ggctcgatcg gctccttcga
     3001 gccccgggag gagtgcccga cactgtggag gtacggccgc ttctcggcga cgatccggcc
     3061 ctgcgcggcc agcgcctcgc gaacggcgac gcgggcctcg aatcggtcca tgccgtcgaa
     3121 cacggtgccg gtgcccgtga tccgcccgga ctcgtccatg atcgaaggca tcggcaggtc
     3181 gtggcgccgc ccgatctcga agtcgttggg gtcgtgcgcc ggggtgacct tgaccgcgcc
     3241 cgtgccgaag ctcgggtcga cgtgctcgtc ggccacgatc ctgagcatgc ggccgaccag
     3301 cggcagttcg atctcccggc cgaccaggtg cgcatagcgt tcgtcgtcgg gatggacggc
     3361 gatcgcggtg tcgccgagca tcgtctcgac gcgggtggtg gcgacgacca gcgagtcctc
     3421 gccgtcgccg tagcggatcg agacgagctc gccctcgacc tcgcggtgtt cgacctcgac
     3481 atcggacagc acgctgcgca gctccggcga ccagttcacc atccgctcgg cacggtagat
     3541 caggccgtcg tcgtagagcc gcttgaagat ggtgttgacg gcgcggctga gcccctcgtc
     3601 catcgtgaag cgctcgcggg accagtccac gctgtcgccg aggcggcgca tctgggccag
     3661 gatggcgccg ccgtgctcgg ccttccactc ccaggtgcgc tcgacgaacg cctcgcggcc
     3721 cagctcgcgt cggctcgtgc cctcggcggc gagctggcgc tcgaccagcg cgtggaccgc
     3781 gatcgacgcg tggtccatgc cggggagcca cagcgtctcg tggccctgca tgcgccgccg
     3841 ccgcacgagg atgtcgatca gggtgtgctc gaacgcgtgg ccgatgtgca ggctgccggt
     3901 gacgttcggc ggcgggatga cgatgcagaa cggcggcttg tcactcgacg cgtcggcggt
     3961 gaagtacccg gcctcgaccc accgctggta catgccggcc tctacctcac ccggattcca
     4021 ctgggcgggc aggtcggcag tgcggggtgg gagggtgtcg gtcacccacc cagtctacga
     4081 gccgggatcg acccggttac ccgcgcaccc acgccggccg gcgggagcag gagggcgtgc
     4141 aggcggtgga agcccagcac gggggcgagc ctgcgctccc gcacgagccg caggtccagc
     4201 gcccgcacct ggccgctcgt gggctcgtag acgcgccagg caccgccgtc gagcacgccg
     4261 agggccatca cgtagtgccg cggcacgagc ggcccgacga gcatcggtac gggcctgccg
     4321 gccgcgaggg cggctcctgc ctcgtcgagc gcgccggtga ggtcagcggc ggacgtgtcg
     4381 tccaccagcc gtactcggta cggccccgcg ccgggaacgt gccgacgcag ccaccgcacc
     4441 atcccccacg gcgtggtccc gagtgcccgc ggccagaacc ggttggtctc ccggtgcacc
     4501 tgccgctgac gcgcgtcgta acgggcgccg aaccccaggc cgcgaccggc ggtggggccg
     4561 tcgaggcgca acatctcggc gggatcggcc cacgccgaca gcgcgacgag caccgcgctg
     4621 ccgcaggtgg cgcggtccac ctggtcgaga cgggcaccgc cccggcgctg ctcgccgatg
     4681 ggctcggtca acaggggcct gcggccaccc gccaacggga gcaccgcagc gacccggccc
     4741 tgtttccgca gaccccgccc gacgagcgac tggatccgcc gcgccatccc gaacagcgtc
     4801 cgcatgccgc gatcctcccc gccgacgggg agtcccgcac gttcctgggc gcctcttccg
     4861 ctcgatggct cagcgcgccg ccgtcaacgt cctcgaccac agctcgccgc cgtcctgccc
     4921 gcgcaaggtc acctgcagct ccccgccgcc cggcgcgacc tcgacctccc cgaagtgctg
     4981 gaacccgtcc atcggagaag tgttcgccgc agggggcgct ttgacgaaca cggcctccgg
     5041 gccgaacgtc gggtccagcg ggttcggccc gaatgccccg gcgtgcagcg ggccggagac
     5101 gaactcccag aactcgtcga actccgtgaa cccgcccgcc gaacccgccc gctcggggga
     5161 gtagtggttc gccgaggtgt agtgcacgtc cgcggtcaac cacaccacgt tgcggacctt
     5221 gcggtcccgc agccccttca gcacccaggc gatctcggcc tcgcggccgc tcggcggccc
     5281 cgccgcgccg ttcgcgacgg cctcccacgc ggtgccgtcg ggcacctgca cgccgatcgg
     5341 catgtccgag gcgacgatct tccaggtggc cttcgacgag gcgagcgcgt cgaccagcca
     5401 gcgcgcctgc cggtccccca gtatccgctc catcggcgca gtgttcgcgc tgttgacatc
     5461 gcggtaggtg cgcatgtcga gcacgaacac ctcgacgtgc ggtccgtacg ggaactgccg
     5521 gtacacccgc ccgtccaccg cgcggcgcgg gtcaagcggc acccactcgt ggaacgcctg
     5581 gaacgcccgc ttcgcgagca cgtcgacgtg cttctcggtg tagcggtcgt cggtaatcat
     5641 ctcgccgggg taccagttgt tgagcacctc gtggtcgtcc cactggttga actgcggcac
     5701 tgccgcggcg aagccacgca ggttgtcgtc gagcaggttg taggcgtact gcccgcggaa
     5761 ctcggcgagc gtctcggcca ccttgagctt ctccggggtc acgacgttgc gccaggtgcg
     5821 cccgtccggc agcgtgacgg tctcggtgag cggtccgtcg gcgtacacgg tgtctccgct
     5881 gtgcaggaag aagtcggggt cacgctccgc catcgctgcg aagatccgca tgccaccgag
     5941 gtcggggttg atgccccacc cctgccccac cacgtcgccc gaccacacga agcggacacc
     6001 gtcgcgcttg cccggcaggg tgcggaacga gccgctcgcg ggctcgctcc cgctacgtcc
     6061 gtcgaggtcc tccgcggtga cccggtagtg cacggtgcgc ccctcccccg gcacacgcac
     6121 gcggccggtg aagtcggtgt ccggggtcag gacggggccg cggaacgtgc gggagcggcg
     6181 gaagtcgggg tcgtcggaca cctcgacgat catgcgcgac ggccggtccg cgcgagccca
     6241 gacgagccca gagccggcca gcacgtcgcc ggactggacg ccgtgggtga gcacgggccg
     6301 gtccgggcgg gcgagggccg gggcggcgaa cgccgacgtg ccggtaccga gcgggacagc
     6361 ggaggcgagc ccgccgatca cggcggtgcg gagcagggtg cggcggtcga cggcatggtg
     6421 ggaacgcatg ggacacatgc aatccgatcg ggtgaaccgg acgccggatc tccgagggga
     6481 cgggcaggtg aactctgcgt cagctctcgg aacgcagtgc gtcgcgacgg tccagccgcc
     6541 gtgacacggc ggccaccgcg atcgctccgg ccgccagcag caccccgaga taggcgggtg
     6601 cggtgtagcc ccagccggcc gcgatcgcga gcccgccgac cgcgggcccg acggagttgc
     6661 cgacgttgaa cgccgaggtg ttggtggcac tcgccagcgt cggggcggcg cccgccagcg
     6721 cgaacaccct cgcgttgagc ggagcacccg cgatgaacgc gacggcgccg aggagcacca
     6781 ccagcccgac gacggccacc ggcacccgcg cgaagaccgc gaccaacccg aggagcacag
     6841 ccgccgccgt gagcccgccg aacagcgtgg gccacgggta cgggtcggcc agtcgcccgc
     6901 cgatcaccac cccggccagg ctgccgatcc cgaagccgag cagcactccg ggcacccagc
     6961 gctcttccag gcccgtgacg tcggtgagca gcggcgcgat gtagctgaac gcaccgatca
     7021 tcgccgactg gtagagcgcc gtggtgccca gcgcgagcca cagcctgccg cggcggaacg
     7081 cggcgacctc ggctgccagc cggggccggg gcatgcccgc cgtccgttgc ggcacggtca
     7141 gcagcacgcc gaccgcactg gccgtggcga gcgcggcaac cgcccagaac gccgagcgcc
     7201 agccgaactg ctgccccacg agcgtgccca gcggaacacc ggcgacgttc gcgaccgtga
     7261 gcccgccgag cagcaccgac agggccatgg cgcgcagctc cggcgccacc acggacaccg
     7321 tgacgacggc ggcgaccgcc cagaacgccc cggtcgcgac ggccgtgacc accctcgcga
     7381 cgagcaacac cccgtacccc tgcgcgaggg cgccgatgac gtgcccgacg acgaaaacgc
     7441 ccagcgctgc cagcagggtg atgcgccgag gcagccgcag cgtcagcacc gccatcgcgg
     7501 gcgcgcccac gatcatcccg acggcgaacg cagagatcag gtaacccgcc tccggtatgc
     7561 cgacgcgcag gtcggccgcc agctccggca gcagcccggc gatcatgaac tcgctggtgc
     7621 cgagcgcgaa tatgctcaac gccagcacgg gcacgacccg cgggatacgg gtcgttcccg
     7681 gcggcgcggc ggcgaccgac atggttcccc ctcgttgcga gacgtacggg caacctaggg
     7741 cgccgtccgt gaattcctgc cggcccgggc cacatgccgc ggaaacgaag tttgcgcggc
     7801 attagagtta gggcatgagc atgatcagcc cggaggagcg ggccttcctg acccacggca
     7861 cgcgcaccgg caagctcggc ttcgtcgccg cggacgggcg gccactggtc gccccgatct
     7921 ggtttgtgct cgagggcacc gacgacgcac ccgaactggt gttcaacacc ggcaagggga
     7981 ctgccaaggg ccgcgctgtc gcgcgcgatc cgcgcctggt gctctgcgtc gacctcgagc
     8041 ggccgccgta cgggttcgtg caggtgcagg gtgaggcgac gatctcggag gaccccgacg
     8101 agctcctgcg cactgccacg gcgatcgcgg cccgctacat gggcgcggac cgggcggagg
     8161 agttcggcag gcgcaacggc gtgcccggcg agctggtggt ccacctgcgg cccacgaagg
     8221 tggtggcgga gctgcacatg accg
//
LOCUS       DASRSC010000115        20292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2982734,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000115 DASRSC010000000
VERSION     DASRSC010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 20292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20292
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2982734"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..525)
                     /locus_tag="VFE39_05445"
     CDS             complement(61..525)
                     /locus_tag="VFE39_05445"
                     /inference="COORDINATES: protein motif:HMM:NF040078.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HET6258079.1"
                     /translation="MADPALHNVYELFAAVVVGLIATVLKFGDRTQIGAVCLAGDRWA
                     ADLVDRMTELSGRHLQALWKLRVNHVEAPALTWWPATGEARLGDLLRDPDDREERLHV
                     VVLMILRDDECLLTPDDDVVLASGDELLLAGLRVQGDGRPVRDVRTGHDRHD"
     gene            complement(702..1880)
                     /locus_tag="VFE39_05450"
     CDS             complement(702..1880)
                     /locus_tag="VFE39_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6258080.1"
                     /translation="MTQARPMPIHMRRDGFDPPAELAAVRDGAGLTRVPTLFGTSAWL
                     VTRHADVREVMADAVRFSNAARPPGFPALGGPDTEGSLLLSDPPEHTRLRKLLSPEFT
                     VRRIRRLEPRITEIVEDHLAAMEAAGPPADLVPAFALPVPSLVICELLGVPYADRAEF
                     QGRAGRQLDVSLDLEERRRAGAESRAYMAELVARTRVEPGEDLLGMLVREHGDDLTSG
                     ELTGIASLLLIAGHETTANTIGLGVLALLRAPDQLRLVRDRSEAVEAAVDELLRYLSV
                     VHSGMPRFATQDTVLAGERIAAGDMVILSLPAANRDPEIGDDLDTLDVTRAITRHVAF
                     GHGVHHCIGAPLARTELRIALPALLRRFPSLALADPEAEIDFRSYNVVYGVRSLPVSW
                     "
     gene            2227..2916
                     /locus_tag="VFE39_05455"
     CDS             2227..2916
                     /locus_tag="VFE39_05455"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6258081.1"
                     /translation="MAEPIDPTTRPDSLSRQIYAALREGIIRGRYPQGSRLAEQRLAE
                     ELQVSRVPLREAVPLLEVDGFVSTLPRRSAIVTTWTITSAHDLFDLRLCLEVGAARYA
                     ARQVAAGASAAPLQAALGSSRDGLGSGDAYRIAGDSTRFHEVVVELTDNALMRSVMRS
                     VSGRMMWLFFMTRELDPLDAHHGHEELLAAIESGNERVAEAVAYTHIERDRLDSMRTL
                     RNSRDILDEPG"
     gene            complement(2977..3138)
                     /locus_tag="VFE39_05460"
     CDS             complement(2977..3138)
                     /locus_tag="VFE39_05460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258082.1"
                     /translation="MVLLNTMAYDSAPGITAAARRPPPRSGIVAHGITNSDSLVGLTG
                     AEERVYTAG"
     gene            3411..3860
                     /locus_tag="VFE39_05465"
     CDS             3411..3860
                     /locus_tag="VFE39_05465"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6258083.1"
                     /translation="MTDSDQNDDSDHRAHHAVSPTDLLTLNATRIGSAAVVTVAGELD
                     LSTARKLMSVVDETLAWPDLAAVVIDLTGVDFLGSSGLGTLAELASRTTTTPGPGAYP
                     APSRTARPALRLVAPPDNHAVIRPWETMNLQQILPLHPDVTAALAGF"
     gene            complement(3879..4922)
                     /locus_tag="VFE39_05470"
     CDS             complement(3879..4922)
                     /locus_tag="VFE39_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6258084.1"
                     /translation="MRTATTVEGSQRGWRETLDFALEAEKLGLDICWVAEAWGSDAPS
                     VLGYLAARTSRMLLGSGVIQLGTRTPVAIAQAALTLAEMSEGRFLLGLGASGPQVIEG
                     LHGVPFAEPRTRMRETVEIIRQALAGEKISYSGTRVQIPLPGGDGRPMRLSIGPNPDI
                     PIYLATMSPRLLQLTGEIADGWLGTSFVPEGSAAYFTHLDAGLAAAGRSRSEIDVCEG
                     AEVAFAEDEDALREMVGARKKELAFSLGGMGSASTNFYNDAYSRQGWAEVAAAVRERW
                     QAGDRDGAAGLVTDEMVLGTTLIGTEEMVAARLRVWRAAGVDTIRLYPAGDTLDVRLT
                     TLARALDLVRSLD"
     gene            complement(4922..5428)
                     /locus_tag="VFE39_05475"
     CDS             complement(4922..5428)
                     /locus_tag="VFE39_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6258085.1"
                     /translation="MEWTGARYADRPTAEGETWIDAPPGRVWAVVSDVELMPAMSTEL
                     QSVEWCDGATGPAVGNRFVGRSKHESLGAWATTSFIVECEEPRVLAWAVENPDHPTAV
                     WRFTLEAADGGTRLREWMQMGPARSGLSFAIDRMPDKEQKIVFVRMREFENAIIGTLA
                     AVKQRVEG"
     gene            complement(5673..8423)
                     /locus_tag="VFE39_05480"
     CDS             complement(5673..8423)
                     /locus_tag="VFE39_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator"
                     /protein_id="HET6258086.1"
                     /translation="MGRGEDVELIRSFLAGAAAGGGALLLVGEPGVGKSVLVDAAVEA
                     ASAAGTLVLCAGGAEFEADVSFSGLNQVLFPLLEQEFDRLTDGHRVALSVALGCGEGP
                     PPDRLVVSNATLGLLRAIGADRPVLLVVDDVHWMDRASAGVLAFVARRLAGSRVGFLA
                     ASRTGMESFFEGAGLPEHELQPLDGDAARTLLEARFPALGVKVRRRVLAEARGNPLAL
                     LELPAALTGPQRAALRALPAVLPLSRRLQTLFVSRISELPEASRQLLLLAALDATVDL
                     RVLQAAVTERGGSDPLAAAERAQLLQVDESTGRLTFRHSLTRSAVVELSTTDQRRRAH
                     LALAEMVDQPERRAWHLGEATIEPDERVASLLEEAAHRIGGRGDPAGAVAALTRAAHL
                     SPLRSERSRRLAVAAFLGVETGEMGTASQLLRDARRADPAHGGGSLYAAATGAYLLSN
                     GDNDIEAAHRLLVEAIENRTESDDDEALVEALWTLSLLCWFGGGAEKWDPYHAALADL
                     GTRVPPLLALLTATFPDPVRTAAPALDQLTTTISSLRDEIDPTRIVRTCNAAVFMDRL
                     AGCRDPLWRVVRHAREGGPLAPAVSALIYLCVDDFITGRWDEIGQLADEGLEMGDAHG
                     YHHFEWPFHYHRALVAAVRGDQETSRIQIDEGLRWATPRGARGIELYIHHAQGLAALA
                     RNDFEDAYRHAVAISPPGVFASHVPVALWVMMDLVEAAVRTDRRAEAAAHVAAMHDAD
                     IAALSPRLALLVAGSTAIAADDDDATVSFERGLAVPGSDRWPFEVARVRLAFGEHLRR
                     ARAPGAARVQLASALETFDRLGARPWAARAGHELRAAGLSTAQPADVGDLTPQEREIA
                     VLAAAGLSNKEIGQQLFLSHRTVGAHLYRVFPKLGITSRAALRDALATLPPRPSRDGA
                     SL"
     gene            complement(9071..9274)
                     /locus_tag="VFE39_05485"
     CDS             complement(9071..9274)
                     /locus_tag="VFE39_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005440412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET6258087.1"
                     /translation="MAQGTVKWFNGEKGFGFISTDDGGADVFVHYSEIDAGGFRSLDE
                     GQRVDFTVGQGAKGPQATGVRVI"
     gene            complement(9492..10493)
                     /locus_tag="VFE39_05490"
     CDS             complement(9492..10493)
                     /locus_tag="VFE39_05490"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET6258088.1"
                     /translation="MTTVTDPGPTARAGPTRAAEHCGPAKPVLDGRRPPGESALVYTF
                     TLIPMLALVAAVPLAWGWGLSWLDVGLAVGFYYLSGFGVTVGFHRYFTHRAFKANRGL
                     RNGLAIAGSLALQGDVITWTADHRRHHAFADKEGDPHSPWLFGTTPAALARGFFHAHL
                     GWLFGRNRTNAARFTPDLLADRDIARISRLFPVWTVLSLLAPALLGGLITMSFWGALT
                     AFFWAGLVRVAVLHHVTWSINSICHMIGERPFAAQGKATNVWPLAILSMGESWHNLHH
                     ADPTCARHGVGPGQVDMSARVIRILEKLGWARDVRWPTTERLARLRADATVTAPTRT"
     gene            10686..11450
                     /locus_tag="VFE39_05495"
     CDS             10686..11450
                     /locus_tag="VFE39_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET6258089.1"
                     /translation="MTSTDEGAHADAARRRGSAAPHGSEGVRIERGAALSRSHVLSAA
                     LALIDADGAEALSMRRLGKAIGRDPMRLYRHAPSKDALLDGVVELVLSELVVPPLHEV
                     GGWEEVLRRTAHAFRAIAVAHPNVVPLLVTRPLATPLAMRPLGTLRPLEKLLELFMGA
                     GFDGRGALHAYRLYVGFLYGHLLNELQERVHDPDESDDLLRLGLHRLPAREFPRLRAL
                     APELAGYDGVQELDEGLDIVLAGLRRQLEADAAGGR"
     gene            11447..12547
                     /locus_tag="VFE39_05500"
     CDS             11447..12547
                     /locus_tag="VFE39_05500"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6258090.1"
                     /translation="MSGRLRIGVVASIAHRTPPRGYGPWEQVASTLTEGLVALGHEVT
                     LFATADSLTSAHLHAEAPRGYEEDPSVDAKVYEGLHNAAAFERADEFDVMSNQFDFLP
                     LTYSRLVSAPVVTTIHGFSSEAILPVYRAYDDIAHYVAISDADRHPDLGYEATIHHGL
                     ELDRFTFRPDSGDYLLFLGRIHPDKGTHLAIEVARRVGRPLVIAGIIQDRDYFRDHVQ
                     PHIGKNDISYAGPVEPGERDVLLGGAFALVHLIGFAEPFGLSVVEALATGTPVIAHPL
                     GSMPELIRHGRTGFLVDDVPGAVDAVGRVGSLVRRDCRDDVEVRFTAERMVADYAELF
                     ARIVAGGPSAVSRPRRSGTSTKANRTDTSLAP"
     gene            complement(12433..13929)
                     /locus_tag="VFE39_05505"
     CDS             complement(12433..13929)
                     /locus_tag="VFE39_05505"
                     /inference="COORDINATES: protein motif:HMM:NF015971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HET6258091.1"
                     /translation="MVTRIDTALTADPARVLARLFVPGHELVVDTESRATGVLARILA
                     LPEDDVRSTLRDVLARYAGRHRNLRGILLAHYERIAHRVPDGAALTEERRNLIGAWFT
                     HEYSIEAAALFNPSAVAHPDQSGLPPGHLRFVLSLRAVGEGHVSSVEFRTGVLGTGPE
                     LSLDDPGQHVEAGRPRPAVHDRALFAAVLAEEGADAESASFLVGGLPPRFDEAELGRA
                     LAALTEQRVTRHGGAHTEELARAIARCSYDVEFSPLSTIAERVLWPHGPSEARGMEDA
                     RFVRVVSDDGSALYRATYTAFDGNRIAPQVIETSDFRRFRVSQLAGPAAKNKGIALFP
                     RTVGGRHFALSRWDRENNAVATSADGRWWGDARTLHVPSRPWELTQTGNCGSPVETEA
                     GWLVLTHGVGPMREYSIGALLLDLEDPSRVIGALREPLMSPRDDERDGYVPNVVYSCG
                     ALLHGDRLLVPYGASDVSVRFAFVDVPLLLGRLTADGPPATMRAKSSA"
     gene            complement(13956..15023)
                     /locus_tag="VFE39_05510"
     CDS             complement(13956..15023)
                     /locus_tag="VFE39_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258092.1"
                     /translation="MSHDSFPSPPFTHLRLLTDEGGLYEHAQGTTPRREHGYCLDDAA
                     RALVVVCREADPEQDDLREQYLSFVLAAQAEDGRFRNRRGTDLRWRGTPSVEDCWGRA
                     LWGLGAAVSDSRSADRALVAFERGARWRAPWSRAMAFAALGAAEVLRVLPQHRGARDL
                     IGAAVQVIGRPGQVPGAGTTWPWPEPRLTYANAALPEALLASGAALDAPALVMDGLQL
                     LGWLLDAETRGGHLSVVPVGGRGPSDAGPGFDQQPIEVAALADACARAHALTGQDRWA
                     AGLDLAAAWFLGANDAGTPLYDPASGGGCDGLERDGRNENQGAESTLALVSTLQQARS
                     RPRVPASRYESARGSAVRGSR"
     gene            complement(15020..16171)
                     /locus_tag="VFE39_05515"
     CDS             complement(15020..16171)
                     /locus_tag="VFE39_05515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6258093.1"
                     /translation="MRVSTRSTRPVASIGLLSTYPPTQCGLATFSAALTEELDRSPGT
                     VGVVRVVDQPETHPGAEVVAHLVNGSPDSAMQAVRHLNGFDAVVVHHEYGIYGGADGA
                     DVIDIVAGLTAPTIIVLHTVLQEPTPRQRRILRRLVESADVLVTMTETARQRLVASYA
                     ADPERVVVIPHGAIDHGVVRTRPRSTGGPLVLTWGLLGPGKGIEWAIDAMPGLRDLRP
                     RYLVMGKTHPKVLQRDGEYYRNSLLRRAEMRGVDDIVELDDRYLGVGELAELVQRADV
                     VLLPYDSREQVTSGVLIEAVAAGRPVVSTAFPHAVELLGDGTGLVVPQRDPEALESAL
                     RRALSEPGLAHDLAVQAARKAPDLLWGAVAGRYQQIATSLVAASMATAS"
     gene            complement(16892..17149)
                     /locus_tag="VFE39_05520"
     CDS             complement(16892..17149)
                     /locus_tag="VFE39_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258094.1"
                     /translation="MTDRVTTLDQRSESRRCSWAEDMAELAPLTVGCSKQVLNAVPEP
                     EVDPGAEKELLAAFEACWTSEDFAEGRRARTEKRPPRFTRR"
     gene            complement(17146..18588)
                     /locus_tag="VFE39_05525"
     CDS             complement(17146..18588)
                     /locus_tag="VFE39_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET6258095.1"
                     /translation="MATTDRNDAVTSDPSQLFPTKYLPRPEVRDLPAPPARQWKYVGP
                     GIVAAGVGLASGEFILFPYIASQVGLTFIWAALVGLITQYFLNMEIERYTLATGETAI
                     TGFSRLWKHWGLVFAILAYFANIWPAWATSSATLISYAFGGSAAVIAVVILVVVGLIL
                     TLAPIVYNALERAQMIKVAAVLVLIVIGSLVAIGPAWADLPQAVTQPRFPAAELGFAV
                     LMGALAFAGAGGGQNLVQSNWIRDKGFGMGHYVPKIVSPLTKEVEARGRVGYVFLPTE
                     QNMTHWRGWWRFANREQLYTFVLISFVSIVFMSLLAYATVFGNDTVTNNIAFLQVEGQ
                     VLMERVGTWFGYFFWIVGAVALFSAALGIVDYTSRLAADVLKTMYFPNVSESKVYAGL
                     VWGLVVIGIIVISAGFSQPITLAVISACTGGTMMFIYSALLIAINRKMLPKPIRIGGF
                     RIAALVWSFLLFGVLAVLTVQQQLGKLLGG"
     gene            complement(18696..18824)
                     /locus_tag="VFE39_05530"
     CDS             complement(18696..18824)
                     /locus_tag="VFE39_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6258096.1"
                     /translation="MPDDAVGAGDAFVAGYLASRLTGHDVQERLSTAAVTGAFAVR"
     gene            complement(18898..19590)
                     /locus_tag="VFE39_05535"
     CDS             complement(18898..19590)
                     /locus_tag="VFE39_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6258097.1"
                     /translation="MPGAAPRNSSTSLRRALSLIEVVATRHAAGGCTLTELAADAGLA
                     RSTVARLVAPLLDHGYVELEPTAGRHRLGAAVARLGSTYLHGPGQRAAALVPPPVPMS
                     EALVTTRVVAILRAEHASRAEAVVDTLVEGGIRCLELTMTTRGALEVVERLAARVPAG
                     VDLGMGTVLTAADVDRPVDAGARFVVSPSVVPAVIAAAADRGIPSATWWSATCWPSGS
                     ARWSGATTRPPL"
     gene            19846..>20292
                     /locus_tag="VFE39_05540"
     CDS             19846..>20292
                     /locus_tag="VFE39_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HET6258098.1"
                     /translation="MTIAQSESDKIYDELIAAHTIMRRGVDLLRTALARTPTAPVEVD
                     SCVGVGRWLIEFVHQHQRTESDLFWQPLADASPTDVGELHDLTVQHIQIDRELDQLAS
                     RLDHLEAGHEAAAAQALRDLDVVRQTLSDHLDEEESLLKALFPMLEP"
BASE COUNT         3149 a         7215 c         7080 g         2848 t
ORIGIN      
        1 gtccgcatcc tgatccctcc tgcccggaca gcccggcccg cgggtgccgc cggccaccgc
       61 tcaatcgtgg cgatcatgtc cggttcgcac atcccgcacc gggcgtccgt cgccctgcac
      121 ccggagcccg gccaggagga gctcgtcccc cgaggccagc acgacgtcgt catccggtgt
      181 cagcagacac tcgtcgtcgc gcaggatcat caggacgacc acgtgcagcc gttcctcacg
      241 gtcgtcggga tcgcgcagga ggtcgcccag ccgggcctcg cccgtcgccg gccaccaggt
      301 cagcgccggc gcctcgacgt ggttgacccg gagcttccac agtgcctgca ggtgccggcc
      361 ggacagctcg gtcatacggt cgacgaggtc ggcggcccag cggtccccgg ccaggcagac
      421 ggcaccgatc tgcgtgcgat cgccgaactt cagcacggtt gcgatgaggc cgaccacgac
      481 ggccgcgaac agctcgtaga cgttgtgcag cgccgggtcg gcgatgtctc accgatgaag
      541 aacccgaagt tcaggggtgg cggcgagcag cgcgaagaag ccgaagacaa ccctctccag
      601 gttcatccgc accccctgcg gtcgtcgcgg tccgggtggt ccggccgcgc cgcggtcacg
      661 ctagcgctgc cggtcccgac acgggggacg gaacggtcgc tctaccagct gaccggcagc
      721 gaccggacgc cgtacacgac gttgtaggag cggaagtcga tctcggcctc ggggtcggcg
      781 agagcgagcg acgggaagcg gcgcaacagg gccggaaggg cgatgcgcag ctccgtgcgg
      841 gccagcggcg cgccgatgca atggtgcacc ccgtgcccga acgcgacgtg gcgggtgatg
      901 gcgcgggtga cgtcgagcgt gtccaggtcg tccccgatct ccggatcgcg gttggccgca
      961 ggcagcgaga ggatcaccat gtcgcccgcc gcgatccgct cccctgcgag gaccgtgtcc
     1021 tgcgtcgcga agcgcggcat gccgctgtgc accaccgaca ggtagcgcag cagctcgtcg
     1081 accgcggcct cgacggcctc ggaacggtct cggaccagcc ggagctggtc gggagcccgc
     1141 agcagcgcca gtacaccgag gccgatggtg ttcgcggtcg tctcgtgccc ggcgatgagc
     1201 agcaggctcg cgatgccggt gagctcaccg gaggtgagat cgtcgccgtg ctcgcgcacg
     1261 agcatcccga gcaggtcctc gcccggctcg acacgcgtcc tggccacgag ctcggccatg
     1321 tacgcccgcg actcggcccc cgcccgccgg cgctcctcca ggtcgaggga cacgtcgagc
     1381 tgccgcccgg cgcgtccctg gaactcggcg cggtcggcgt aggggacgcc gagcagctcg
     1441 cagatcacca gcgacgggac cggcagcgcg aacgccggaa ccaggtcggc gggaggtcct
     1501 gccgcctcca tcgcggcgag atgatcctcg acgatctccg tgatcctcgg ctcgagccgc
     1561 cggatccgcc gcacggtgaa ctcgggactc aggagcttcc gcagccgggt gtgctcgggc
     1621 gggtcgctca gcagcaacga cccttcggtg tcggggccgc cgagcgcggg aaacccgggc
     1681 ggccgggcag cgttgctgaa ccggaccgcg tcggccatca cctcgcggac gtcggcatgg
     1741 cgggtgacga gccaggcgga ggttccgaac agcgtcggca cccgggtcag acccgccccg
     1801 tcccgcaccg ccgcgagctc cgcgggcggg tcgaagccgt cccgtcgcat gtggatcggc
     1861 atcggtcgcg cctgcgtcat cgcgggtgtc ccctccgttc ggcacagcgc cggacggcgc
     1921 gggctcgtac ctccttcgtt acccgtcccg aggcaccggc gccaccacgc gggcccggag
     1981 ggcctcagag ttctctcagt gccgacagtg tcaggtagcg gaccgggcgc gacaaacgac
     2041 gacgtcaccc gtcgcggttc tcagagctcc gctgtggcgg cggccgcggc tgcccgctaa
     2101 ccggtcgcct tcggctgcag tgaaatctcc gtggcggcgt cgaagatcga caccagcgac
     2161 tcgtgcagga aggtcaagag ccgcgcgcgc cttggagcca ccggcgcagt gcaggacaat
     2221 tcggccatgg cggagccgat cgaccccacg acgaggcctg actcgctctc gcgccagatc
     2281 tatgccgccc ttcgtgaggg catcatccgt ggccgctacc cccagggatc ccggctcgcg
     2341 gagcagcgtc tcgccgagga gctgcaggtc tcgcgcgtcc cgctgcgcga ggcggtgccc
     2401 ctgctggagg tcgacgggtt cgtcagcacg ctgccccgcc gcagtgcgat cgtcacgacg
     2461 tggacgatca cgtcggcaca tgacctgttc gacctgcgac tgtgtctcga ggtgggcgcc
     2521 gcccgctacg ccgcgcggca ggtcgccgcc ggcgcgtccg ccgcgccgct gcaggccgca
     2581 ctcgggtcct cccgcgacgg gctgggcagc ggcgacgcct accgcatcgc cggcgacagc
     2641 acccggttcc acgaggtggt cgtcgagctc accgacaacg cgctgatgcg ctcggtgatg
     2701 cgctcggtct cggggcggat gatgtggctg ttcttcatga cacgggagct cgacccgctc
     2761 gacgcccacc acggccacga ggaactgctc gccgccatcg agtccggcaa cgagcgcgtg
     2821 gcggaggccg tggcctacac ccacatcgag cgggaccggc tcgactcgat gcggaccctg
     2881 cgcaactccc gcgacatcct cgacgagccc ggctgacgtc ctcccagcac ggtggcgata
     2941 cgggcccaac cccgcggcga gctcgagcac ccgcggtcag ccggcggtat acacccgctc
     3001 ctcggcccca gtgaggccga cgagcgagtc ggagttggtg atcccgtgcg cgacgatccc
     3061 gctccgtggc ggcgggcggc gggcggcggc ggtgatgccg ggggccgagt cgtaggccat
     3121 cgtgttcagc aggaccatcg gggggatccc gtaccgcccc agccggtcga ggaacggaag
     3181 gcgccgaacc ggtagtcctc cacgcccgcc tcgacgacga tctgccgtcg acggggaggc
     3241 ggtcatgcca aaccgtatac agtttttgag tttctggcgg atgacggcgg atgacggcgg
     3301 aaagactgcg tcgtcccgga cccggaccac ggtgacacca ggtgagtcga ggcgtcctcg
     3361 agcgtcacag cggacccagc cgggcagcgt cgagacgcta cggtgctcgc gtgaccgaca
     3421 gtgaccagaa cgacgacagc gaccatcgtg cccaccacgc ggtgtcaccc accgacctgc
     3481 tgacactgaa cgcgacccgt atcgggtcgg cggccgtggt gacggtggca ggcgagctcg
     3541 acctgagcac cgcgcgcaag ctgatgtccg tggtcgacga gacgctggcg tggcccgacc
     3601 tcgccgcggt ggtgatcgac ctgaccgggg tggacttcct cggctcctcc gggctcggga
     3661 ccctcgctga gctggcctcc cgcaccacga cgacccccgg acccggtgcg tacccggcgc
     3721 cgagccgcac cgcacggccg gctctgcgcc tcgtggcacc ccccgacaac cacgccgtga
     3781 tccgcccgtg ggagacgatg aacctgcagc agatcctccc actgcacccc gatgtcaccg
     3841 ccgccctcgc cggattctga gcggggggcg tgggtgagtc agtcgagaga ccggaccagg
     3901 tccagcgccc tggcgagcgt ggtcagccgg acgtcgagag tgtccccggc gggatagagc
     3961 cggatggtgt cgacgccagc ggcgcgccac acgcgcagcc gggcagccac catctcctcg
     4021 gtgccgatca acgtggtgcc cagcaccatc tcgtcggtga cgagccctgc ggccccgtcc
     4081 cggtctccgg cctgccaacg ctcgcggacg gccgcggcca cctccgccca gccctgccgg
     4141 ctgtaggcgt cgttgtagaa gttggtgctc gcggagccca tgccgccgag gctgaacgca
     4201 agctccttct tgcgagcgcc caccatctcg cgcagcgcgt cctcgtcctc tgcgaacgcg
     4261 acctcggccc cctcgcaaac gtcgatctcg ctgcgggacc ggccggccgc ggccagtccg
     4321 gcgtccaggt gcgtgaagta ggcggccgag ccctcaggga cgaagctggt gccgagccac
     4381 ccgtccgcga tctccccggt cagctgcagc aaccggggcg acatcgtggc gagatagatc
     4441 gggatgtcgg ggttcgggcc gatcgacagg cgcatcggcc tgccgtcgcc gcctggcagc
     4501 gggatctgga cccgggtgcc cgagtaggag atcttctccc cggccaacgc ctgccggatg
     4561 atctccacag tctcccgcat ccgggtgcgc ggttcggcga agggcacccc gtgcaacccc
     4621 tcgatcacct gcggacccga cgcgcccagg ccgagcagga accgcccctc cgacatctcg
     4681 gcgagtgtga gggccgcctg cgcgatcgcg acaggcgtgc gggtgccgag ctggatgacc
     4741 cccgagccga gcagcatccg gctggtccgt gcagcgaggt aaccgagcac ggacggcgcg
     4801 tcggagcccc aggcctcggc gacccagcag atgtcgaggc cgagcttctc ggcctcgagt
     4861 gcgaagtcga gcgtctcccg ccagccccgc tgagagccct cgacggtggt ggcggtgcgc
     4921 atcaaccctc gacccgctgc ttgaccgcag cgagcgtgcc gatgatcgcg ttctcgaact
     4981 cccgcatcct gacgaacacg atcttctgct ccttgtcggg catccggtcg atcgcgaagc
     5041 tcagccccga gcgggcaggc cccatctgca tccactcccg cagccgcgta ccgccgtcgg
     5101 cggcctcgag ggtgaaccgc cagacggctg tcgggtggtc cgggttctcc accgcccacg
     5161 cgagcactcg cggttcctcg cactcgacga tgaacgacgt cgtcgcccac gccccgagcg
     5221 actcgtgctt gctgcgaccg acgaaccggt tgcccaccgc ggggccggtg gccccgtcgc
     5281 accactcgac cgactggagc tcggtgctca tcgccggcat cagctcgaca tcggagacga
     5341 ccgcccacac ccgtcccggc ggggcgtcga tccaggtctc gccctcggcc gtcggccggt
     5401 cggcgtaccg ggcgccggtc cactccattt cgtgcgcact cctcccctag cgagccgctt
     5461 agcgccggtt agtccggtcg gttcacccct gtcgaccggc gaacctccgg ctgctccgcc
     5521 ggcctgcccg gatagcccgc tggacgtgtc ctcggccgtc agcggctcca agcattgggc
     5581 ccagacgacc tcgccgtgcg tctcgtggtc gcagcgaagg atgtccgcct catgccccgg
     5641 agcatacggg tacgacgatg agactcagcg gctcacaagc tggcgccgtc gcgggacggc
     5701 ctgggcggca gggtggcgag cgcgtcgcgc agcgcggctc gcgaggtgat accgagcttg
     5761 gggaagaccc ggtagagatg cgcgcccacc gtgcgatggg acaggaacag ctgctgcccg
     5821 atctccttgt tggacaggcc agcggcagcc agcacggcga tctcccgctc ctgcggcgtg
     5881 agatcgccga catcggcggg ctgggccgtg ctgagaccgg cggcccggag ctcatgaccc
     5941 gctcgggccg cccacggacg cgctcccagc cggtcgaacg tctcgagcgc agaagcgagc
     6001 tgcacgcgcg ccgcgccagg ggcccgagcc cgacgcagat gctcgccgaa ggccagccgc
     6061 acccgggcga cctcgaacgg ccagcggtcg gaaccgggaa cggcaaggcc ccgctcgaac
     6121 gacaccgtgg catcgtcatc gtctgctgcg atggccgtcg accccgcaac cagaagagcg
     6181 agccgcggcg acaacgccgc gatgtccgca tcgtgcatgg ccgctacgtg cgcggccgcc
     6241 tcggcccgcc gatcggttcg gacggcggcc tcgaccaggt ccatcatcac ccacagggca
     6301 actggcacat gcgacgcgaa gactcccggc gggctgatcg ccaccgcatg ccggtaggcg
     6361 tcctcgaagt cgttccgcgc gagggcagcc aacccctggg catggtggat gtacagctcg
     6421 atgcctcgag ccccgcgcgg cgtcgcccag cggagaccct cgtcgatctg gatccgagaa
     6481 gtctcctggt caccacgtac ggcagccaca agggcgcggt ggtagtggaa cggccactcg
     6541 aagtgatggt atccgtgcgc gtcacccatc tcgaggcctt cgtcggccag ctgcccgatc
     6601 tcgtcccacc ggcccgtgat gaagtcatcc acgcagaggt agatcaacgc actgacggcc
     6661 ggggcgagcg gaccgccctc gcgcgcgtgg cggacgacgc gccacaacgg gtcgcggcaa
     6721 cccgccagcc gatccatgaa gacggccgcg ttgcaggtcc gcacgattct tgtcgggtcg
     6781 atctcgtcgc gcaggctgct gatcgtggtg gtgagctgat cgagcgccgg ggcggcggtg
     6841 cgtaccggat cggggaacgt ggcggtcaac agggccagga gcgggggcac gcgcgttccc
     6901 aggtcggcga gggccgcgtg gtagggatcc cacttctcgg caccgccgcc gaaccagcac
     6961 agcaacgaga gggtccacag cgcctcgacg agtgcttcgt cgtcgtcgga ctcggtccga
     7021 ttctcgatcg cctcgaccag cagtcggtgg gcggcctcga tgtcgttgtc accgttgctc
     7081 agcagatagg ccccggtggc cgcggcgtac agcgatccgc ctccgtgcgc agggtcggca
     7141 cgccgggcgt ctcggaggag ctgcgatgcc gttcccatct caccggtctc cacgccgagg
     7201 aacgcggcca cggccagccg tctgctccgc tcggaacgga gcgggctcag gtgagcggcc
     7261 cgggtcaacg cggcgacggc tccggcggga tcccctcggc cgccgatccg gtgagcggcc
     7321 tcttcgagca ggctagccac ccgttcgtcg ggctcgatgg tcgcttcgcc caggtgccac
     7381 gcccggcgct cgggctggtc caccatctcg gccagggcca ggtgggcccg ccgccgctgg
     7441 tcggtggtcg acagctccac gaccgccgaa cgggtcagcg agtggcggaa ggtaagccgg
     7501 ccggtgctct cgtcgacctg cagaagctgg gcacgttcgg cggccgcgag cgggtcgctc
     7561 ccgccccgct cggtgacggc cgcctgcaac acgcggagat cgacggtggc gtccagcgcg
     7621 gcgagcagaa gcagttgcct ggacgcttcg ggaagctccg agatccgcga tacgaacagc
     7681 gtctgcagcc gtctactcag cggaaggacc gccggcaggg cccgcagtgc cgcacgctgc
     7741 ggaccggtca acgccgcagg cagctcgagc agtgccagcg ggttgccccg cgcctccgcg
     7801 aggacacggc gacggacctt caccccgagc gcaggaaacc gggcctccag cagcgtcctc
     7861 gccgcatcac catcgagcgg ctgcaactcg tgctcaggaa gtcccgcgcc ctcgaagaag
     7921 ctctccatcc cggtgcgcga cgctgccaga aaacccaccc ggctaccggc gagtcgacgc
     7981 gccacgaacg cgagcacgcc ggcactcgcc cggtccatcc aatgcacgtc gtcgacgacg
     8041 agcagcaccg gccggtcggc accgatcgca cgcaacaacc ccagcgtcgc gttcgacacc
     8101 accaaccggt cagggggcgg gccttccccg catcccagcg ccactgacag cgccacccgg
     8161 tgcccgtcgg tgagccggtc gaactcctgc tccagcaacg ggaacagaac ctggttcaac
     8221 cccgagaaac tgacgtccgc ctcgaactcg gcgccacccg cacacaggac cagcgtcccc
     8281 gctgccgacg ccgcctccac tgcagcgtcc accaggaccg acttgcccac tcccggctcc
     8341 ccgaccagga gcaatgcccc gccccctgcc gccgctcccg ccaggaagga ccggatcagc
     8401 tcgacgtcct cgccgcgtcc caccagggcg gcaccaatgc cgcctcgacg cgggccagga
     8461 tcgctcaaca cacagcctcc agtgatgtga aggtcaggat tcgcgccgag caggggctcc
     8521 cgaccggccg gccgcgtctg accgctgcgc cgaggtcccg ctgcgggacc ggcgcatgcc
     8581 gcgagaccga aggtttcgtg acagctacaa cggtggaacc cggcagtttc cggaacccga
     8641 cgccgcaaag cgcccgggtt atcacgatca cctcggcagg caacagaacc ttaatctgcg
     8701 cgatcgatcc tcagcaagcc gccagccggg tatccggaga tcgttccgca gccctgtcgg
     8761 cgccgggccc gcaaccgtcg tggcgcgcga cgtatccacg ctccggcgcc gtgatcaggc
     8821 ctctgaccag tatgaactca tgacggccgc ggccgatcac cgccgcggcg agccggtctc
     8881 gcgatgccac gaaacggacc cacaagggac tacaccgaga tcacgatggc cgcgtcggac
     8941 acgtggcgaa tgccacgtcg agcgggcgaa gatgtcgtaa ctgtcggtct tctcctttct
     9001 ggcccgcagt tccggaaacg acaagagccc gcttccctcg aggaagcggg ctcttgaccg
     9061 agcagataga tcagataacg cgcactccgg tggcctgggg gcccttggcg ccctggccga
     9121 ccgtgaagtc cacgcgctgc ccctcgtcga gcgagcggaa cccgccggca tcgatctcgg
     9181 agtagtgcac gaagacgtca gcgccaccgt cgtcggtgga gatgaaacca aagccctttt
     9241 cgccgttgaa ccacttcaca gttccctgtg ccattttgtc tgctcctcgc aggtgggtgg
     9301 gctcgcaatg tgcggggccc ggccgacctc ggtgccgatg cccggcgtcc ttctgacacc
     9361 ggatctctgg caccaaattt cgttcccgcg ggcgtgcaag acacgaacac cgaaactcaa
     9421 cgaccaactc tatccaacca cagagcggcc agatctgttc ggcgggtcac cttttagagt
     9481 tttcggtcgg gtcaagtccg cgtcggagcg gtcacggtcg cgtcggccct gagccgggcc
     9541 aggcgctcgg tggtgggcca gcggacgtcg cgtgcccacc ccaacttctc caggatccgg
     9601 atgactcggg cggacatgtc gacctgtccg gggccgacgc cgtgccgggc gcaggtgggg
     9661 tcggcatggt gcaggttgtg ccaggactcg cccatcgaca ggatcgccag cggccacacg
     9721 ttggtggcct tgccctgggc ggcgaacgga cgttcgccga tcatgtggca gatcgagttg
     9781 atcgaccacg tgacgtggtg cagcacagcc acacggacca gaccggccca gaagaacgcc
     9841 gtcaacgcgc cccagaacga catggtgatc agacccccga gcagcgccgg ggcgagcagg
     9901 ctcagcacgg tccacaccgg gaacagccgg ctgatccgcg cgatgtcgcg gtcggcgagc
     9961 aggtccgggg tgaagcgagc ggcgttggtg cggttgcggc cgaacaacca cccgaggtgg
    10021 gcgtggaaga agcccctggc caaggcggcg ggggtggtgc cgaacagcca cggcgagtgc
    10081 gggtcgcctt ccttgtcggc gaaggcgtgg tggcggcggt ggtcggcggt ccaggtgatc
    10141 acgtcgccct gcagcgccag gctccccgcg attgcgagcc cgttgcgcag cccccggttc
    10201 gccttgaacg cgcggtgggt gaagtagcgg tggaacccga ctgtcacgcc gaagccgctg
    10261 aggtagtaga agccgacggc gagtccgacg tcgagccagc tgaggcccca gccccaggcg
    10321 agcggaacgg ccgcgaccag ggccagcatc gggatcaggg tgaaggtgta gaccagcgcg
    10381 ctttcgcctg ggggccgccg cccgtcgagt acgggcttgg cgggaccgca atgctcggcg
    10441 gcgcgagtcg ggccggcgcg ggcggtaggc ccggggtcgg tcactgtcgt catgcttggg
    10501 gttctcctcg gtgtatcgca catgggcggt cgcacgcggc cggtccgcgg cggcggagcc
    10561 gacggcggcc ttcgaggtcg ataccccggc gctgtagcgc tacgagcgac cgtaatacgt
    10621 agcgctacgg tgtagacgct acggtgtagc ccagagttct tcaactggag gtggggaagc
    10681 gcacgatgac gagcacggac gaaggcgcac acgcggacgc cgcacggagg cgaggttcgg
    10741 cagcacccca cggcagcgag ggcgtccgga tcgagcgagg cgcggcgttg tcccgcagtc
    10801 acgtgctgtc cgccgcgctc gcactcatcg acgccgacgg ggcggaggcg ctgtccatgc
    10861 gtcggctggg caaggcgatc ggccgcgatc cgatgcggct gtaccggcac gcacccagca
    10921 aggacgccct gctggacggg gtggtcgagc tcgtcctgtc cgagctcgtc gtcccgccgc
    10981 tgcacgaggt cggcggctgg gaggaggtgc tgcgacgcac ggcgcacgcc ttccgcgcga
    11041 tcgccgtcgc ccacccgaac gtcgtgccgt tgctggtgac ccggccactg gccaccccgc
    11101 tggcaatgcg cccgctcggg acactgcgcc cgctcgagaa gctgctggag ctgttcatgg
    11161 gcgccggttt cgacggtcgc ggcgcgctgc acgcataccg gctctacgtc ggtttcctgt
    11221 acggccacct gctcaacgag ctgcaggagc gtgtgcacga cccggacgag agcgatgacc
    11281 tgctgcgcct gggactgcac cggttgcccg cgcgcgagtt cccacggttg cgcgcactgg
    11341 cccccgagct agccggctac gacggcgtgc aggagctcga cgagggcctc gacatcgtcc
    11401 tggcgggtct gcggcggcag ctggaagcag acgcggcagg cggccggtga gcggccggct
    11461 ccggatcggg gtggtcgcct cgatcgccca tcgcacgcct cctcgcggat acgggccgtg
    11521 ggagcaggtg gcgtccacgc tcaccgaggg gctggtggcg ctcggtcatg aggtcaccct
    11581 gttcgcgacc gccgactcgc tcacctccgc gcacctgcac gcggaggcgc cgcgggggta
    11641 cgaggaggac ccgagcgttg acgccaaggt gtacgagggc ctgcacaatg ccgctgcctt
    11701 cgagcgggcc gacgagttcg acgtgatgtc caaccagttc gacttcctgc cgctgaccta
    11761 cagccgcctc gtgtcggccc cggtggtgac cacgatccac ggcttctcgt ccgaagcaat
    11821 cctgccggtc taccgcgcgt acgacgacat cgcccactac gtcgcgatca gcgatgccga
    11881 ccgtcatccg gatctgggct acgaggcgac gatccaccac ggcctcgagc tggaccggtt
    11941 caccttccgc cccgattcgg gcgactatct cctcttcctg ggaagaatcc accccgacaa
    12001 ggggacgcac ctggcgattg aggtcgcccg ccgcgtggga cggccactcg tcatcgcggg
    12061 gatcatccag gaccgggatt acttccgtga ccacgtgcag cctcacatcg gaaagaacga
    12121 catcagctac gccggccccg tcgagccggg cgaacgggat gtgctattgg gcggcgcatt
    12181 cgcgctggta cacctcatcg gcttcgccga acccttcggg ctctccgtcg tcgaggcgct
    12241 ggccacaggc acaccggtga tcgctcatcc cctgggctcc atgcccgagc tgatccgtca
    12301 cgggaggacc ggcttcctgg tcgacgacgt cccgggagcg gtcgacgccg tcggccgcgt
    12361 cgggtccctc gtccggaggg actgccgcga cgacgtcgag gtccgcttca cggcggagcg
    12421 catggtggcg gactacgccg agctcttcgc ccgcatcgtc gcgggcggtc cgtcggccgt
    12481 cagccggccg aggaggagcg gaacgtcgac gaaggcgaac cgcacggaca cgtcactggc
    12541 cccgtagggc accaggagtc ggtccccatg caggagggcg ccgcaggagt acacgacgtt
    12601 cgggacgtac ccgtcccgtt cgtcgtcccg ggggctcatg agtggctcgc gcagcgcgcc
    12661 gatcacccgg ctcgggtcct ccaggtcgag caggagggcc ccgatggagt actcgcgcat
    12721 ggggccgacc ccgtgcgtca ggacaagcca tccggcctcg gtctccaccg gcgagccgca
    12781 gttcccggtc tgggtgagct cccatgggcg gctcgggacg tgcagagttc gtgcatcacc
    12841 ccaccaccgc ccgtcggccg aggtggcgac agcgttgttc tcccggtccc agcgcgagag
    12901 ggcgaaatgg cggccgccga cggtgcgggg gaacagggcg atccccttgt tcttcgcggc
    12961 cggtccggcg agctgggaca cccggaaccg gcggaagtcc gacgtctcga tcacctgcgg
    13021 cgcaatccgg ttgccgtcaa aggcggtgta tgtcgcccgg tagagcgcgc tcccgtcgtc
    13081 ggaaacgacg cggacgaagc gcgcatcctc catgccgcgc gcctccgagg ggccgtgcgg
    13141 ccacagcacc cgttcggcaa tcgtggacag cggagagaac tcgacgtcgt agctgcaccg
    13201 ggcgatagcc cgcgccaact cctctgtgtg ggctccgccg tgtcgggtga cccgctgctc
    13261 ggtcagtgcc gcaagggccc gccccagctc ggcctcgtcg aagcggggag gcaggccacc
    13321 gacgaggaac gaggcgctct cggcgtcggc gccctcctcg gcgagcaccg ccgcgaacag
    13381 cgcccggtcg tggaccgcag gtcgggggcg cccggcctcg acgtgctgcc cggggtcgtc
    13441 gagggacagc tcggggccgg tgcccagcac cccggtgcgg aactccacgg acgagacgtg
    13501 gccctcgccc accgcgcgca ggctcaggac gaaccggagg tggccgggtg gtaagccgga
    13561 ctggtccggg tgggccacag ccgaagggtt gaacagcgcc gcagcctcga tcgagtactc
    13621 gtgggtgaac cacgcgccga tgaggttacg gcgctcctcg gtcagcgcgg cgccgtcggg
    13681 cacacggtgg gcgatccgct cgtagtgggc gagaagtatg ccgcgcaggt tccggtgcct
    13741 gccggcgtag cgggcgagca cgtcccgcag cgtcgatcgg acgtcgtcct cgggaagcgc
    13801 gagtatccgc gccagtacac cggtggcgcg ggactcggtg tccaccacga gctcatggcc
    13861 cggaacgaac agccgggcca gcacacgtgc cgggtcggca gtgagagcgg tgtcgatgcg
    13921 cgtgaccatc ccggccccga cctccgtcgg tggtctcacc gcgagccgcg cactgccgac
    13981 ccgcgcgcag actcgtaccg actcgcgggg acccgggggc gcgacctggc ctgctgcagg
    14041 gtcgacacca gcgccagggt ggactccgcc ccctggttct cgttgcgacc gtcgcgctcg
    14101 aggccgtcgc atccaccgcc gctggcgggg tcgtacagcg gcgtgcctgc gtcgttggcg
    14161 ccgaggaacc aggccgccgc gaggtcgagc ccggcggccc accgatcctg tccggtgagg
    14221 gcgtgcgccc gggcacaggc gtcggcgagc gcggccacct cgatcggctg ctgatcgaat
    14281 ccggggcccg cgtccgacgg cccacggccc ccgacgggga cgacggacag gtgcccgccg
    14341 cgggtctccg cgtcgagcag ccagccgaga agctgcagcc cgtccataac gagggcgggg
    14401 gcatccagcg cagcgccgga agcgagcaac gcctccggca gggcggcgtt ggcgtaggtc
    14461 aggcgcggct cgggccacgg ccatgtcgta cctgcgccag ggacctgtcc gggacggccg
    14521 atcacctgga cggccgcgcc gatcaggtcc cgggcacccc ggtgctgcgg cagcacgcgg
    14581 aggacctcgg cggcgccgag agccgcgaag gccatggccc gcgaccacgg ggcccgccac
    14641 cgcgcgccac gctcgaacgc gacgagggcc cggtcagcag aacgagagtc ggagacagcc
    14701 gcgccgagtc cccacagcgc cctgccccag cagtcctcaa ccgacggtgt gccccgccaa
    14761 cgcagatcgg tgccccgccg gttgcggaac cggccgtcct cggcctgcgc ggccagcacg
    14821 aatgacaggt actgctcgcg gaggtcgtcc tgctcagggt cggcctcgcg gcagacgacc
    14881 accagcgccc gcgcagcgtc gtcgaggcag tagccgtgct cccgacgcgg cgtggtgccc
    14941 tgtgcgtgct cgtagagccc gccctcgtcg gtgagcaggc gcaggtgcgt gaagggcggg
    15001 gaggggaagc tgtcgtggct cacgaggcgg tggccatcga cgcggccacg agcgaggtcg
    15061 cgatctgctg gtaccgccca gccacggctc cccagagcag gtccggcgcc tttcgcgcag
    15121 cctggaccgc caggtcgtgg gcgagccccg gctcgctcag cgcccggcgc agggccgact
    15181 ccagcgcctc cgggtcccgt tgcggcacca cgagccccgt cccgtccccc aggagctcga
    15241 cagcgtgcgg gaacgccgtg gagacgaccg gacgcccggc cgccacagcc tcgatgagta
    15301 ccccggacgt cacctgctcg cgcgagtcat agggaagcag caccacgtcg gcacgctgca
    15361 ccagctcggc gagctcgccg accccgaggt acctgtcgtc gagctcgacg atgtcgtcca
    15421 cgccccgcat ctcggcgcgc cggagcaggc tgttgcggta gtactcgccg tcgcgctgga
    15481 gcaccttcgg gtgcgtcttg cccatgacga gatagcgcgg ccggaggtcc cgcaggccgg
    15541 gcatggcgtc gatcgcccac tcgatgccct ttcccggtcc gagcaggccc caggtgagga
    15601 cgagtggacc accggtggaa cgagggcgcg tgcggacgac gccgtggtcg atggctccgt
    15661 gcgggatcac gacgacgcgc tccgggtcgg cggcgtagct cgccaccagc cgttggcgcg
    15721 cggtctcggt cattgtgacg agcacgtcgg ccgactcgac gagccgtcgg aggatgcggc
    15781 gctgcctcgg cgtcggctcc tgcagcaccg tgtgcaggac gatgatcgtc ggcgcggtca
    15841 ggccggccac gatgtcgatc acatcggccc cgtccgcacc gccgtagatc ccgtactcgt
    15901 ggtggaccac cacggcgtcg aagccgttga ggtggcgaac cgcctgcatc gcgctgtccg
    15961 gtgagccgtt gacgaggtgc gccacgacct ccgcgccggg atgggtctcg ggctggtcga
    16021 cgacccggac gacgccgacg gtcccgggcg agcggtccag ctcttcggtg agggcagcgc
    16081 tgaacgtggc gagaccgcac tgggtcggtg gataggtgct cagcagcccg atgctcgcaa
    16141 cggggcgggt ggagcgggta ctgacacgca tggacggctc tcctgacgtc ggcgaaacct
    16201 gatgtgagcc gagtgggcta cgcgtcgtca gtgacggtga gcacgcacgg gtctaacgcc
    16261 gtccccggag aggatcggtt ttcacaggag caaccgccga cggacagtcg ggttgattct
    16321 ggtcagcgcg cagcccagga cccgggtgcg ctcacgtcgc cgttcccgcg ctcagggagt
    16381 gcggcctcgg tagggcatga ccccggtccg agccccgccg ttcgtcgtgg agtcgccggc
    16441 gcagacgcat gctcgttgga gcccgcctgc cgccgacgcg gtgcagtacc ggatcccgat
    16501 ccggtcatgg gcatcccacg cgtgcgggca cacggcgcag gcggggtggg atgagtcgtc
    16561 agtacccggc tcggcgacgg tcaccttcgc gtcgatcgct ggcatcttcc gcgttcctgt
    16621 ctctcggacg atcatgcggt ccccggcgaa cggtgctccc gggtctgtgc gggacgagca
    16681 aggaaacgtc ggcgtgttcg ctggaatcgc gaacagttgc tggagcgtca tgcctgccgg
    16741 cgcaacgggt ctcgggcgat gccgcgtcac gagagacgcg accacacgtc actctacgcc
    16801 cggctgatcc ggatccaccc cgcgcaggac ctcttcagat cggccgcgct cacgatccga
    16861 cggcgccggg ctcagggcgc atttcatcgc gtcacctcct cgtgaaccgc ggcgggcgct
    16921 tctcggtgcg ggcgcggcgc ccctccgcga agtcctcgct ggtccagcac gcctcgaacg
    16981 cggcgaggag ctccttctcg gcgccggggt cgacctccgg ctcgggcacg gcgttgagga
    17041 cctgcttgga gcagccgacc gtgagcggcg cgagctcggc catgtcctcg gcccagctgc
    17101 acctgcggct ctccgaccgc tgatccagcg tcgtgaccct gtcggtcaac cgccgagcag
    17161 cttgccgagc tgctgctgca ccgtgagtac ggcgaggacc ccgaacagca ggaacgacca
    17221 gaccagggca gcgatccgga acccgccgat gcggatgggt ttcggcaaca tcttgcggtt
    17281 gatcgcgatc agcagcgcgg agtagatgaa catcatcgtg ccgccggtgc aggcggagat
    17341 gaccgccaat gtgatcggct gggagaagcc ggcggagatg acgatgatcc cgatgacgac
    17401 caggccccac acgagccccg cgtagacctt gctctccgag acgttgggga agtacatggt
    17461 cttcagcacg tcggcggcga gcctgctggt gtagtcgacg atgcccagag ctgcggagaa
    17521 cagcgccacc gcaccgacga tccagaagaa gtagccgaac caggtgccca cgcgctccat
    17581 caacacctgc ccctccacct ggaggaacgc gatgttgttc gtcaccgtgt cgttgccgaa
    17641 gaccgtggcg taggcgagca gcgacatgaa cacgatcgac acgaacgaga tcagcacgaa
    17701 cgtgtacagc tgctcccggt tggcgaaacg ccaccagccc cgccagtgcg tcatgttctg
    17761 ctcggtcggc aggaacacgt agcccacgcg ccccctggcc tccacctcct tggtcagagg
    17821 cgacacgatc ttcgggacgt agtggcccat gccgaagccc ttgtcccgga tccagttgct
    17881 ctgcaccagg ttctggccgc cgccggcacc ggcgaacgcc agcgcgccca tcagcacggc
    17941 gaatccgagc tcggctgcgg ggaaccgggg ctgggtgacg gcctggggca ggtcggccca
    18001 cgccggcccg atggcgacga gcgaaccgat gacgatcagc accagtaccg cggcgacctt
    18061 gatcatctgg gcgcgctcga gcgcgttgta gacgatcggc gccagggtga ggatgagccc
    18121 gaccacgacg aggatcacca ccgcgatgac ggccgcgctc cccccgaacg cgtaggagat
    18181 cagcgtcgcg gagctggtgg cccacgccgg ccagatgttg gcgaagtagg cgaggatcgc
    18241 gaacaccagg ccccagtgtt tccacagtcg gctgaagccg gtgatcgccg tctcgccggt
    18301 ggccagggtg taccgctcga tctccatgtt gaggaagtac tgggtgatca gaccgaccag
    18361 tgcggcccag atgaaggtca gaccgacttg ggaagcgatg taggggaaga ggatgaactc
    18421 cccgctcgcc agcccaacgc ccgcggcgac gatgcccggc ccgacgtact tccattgccg
    18481 cgcgggcggg gcgggcaggt cgcggacctc gggtcggggc aggtacttgg tcgggaacag
    18541 ctgcgaggga tcggaggtga ccgcgtcgtt gcgatccgtg gtggccatcg tgtgtctcct
    18601 tgcacgtacg acaattcggg cgtgatcctc agcggtgaac cgtcccctcc gcggtcagca
    18661 gagtcaactc gtggggccgg ggacgtgcct cccagtcacc gcaccgcgaa ggctcccgtg
    18721 accgcggcgg tggacaaccg ctcctgcacg tcgtgcccgg tgagccggga ggcgaggtag
    18781 cccgcgacga aggcgtcacc ggcaccgacg gcgtcgtcgg gcacaggtgg gagcctgcgc
    18841 tgccgtgccg gtagtagtac acgtgcgagg aggtatcggt gcgccgctcg cgcagcatca
    18901 gagcggtggg cgcgtcgtcg cgcctgacca tcgcgcggac ccgctcggcc agcaggtggc
    18961 gctccaccag gtcgccgatg ggatgccacg gtcggcggcg gccgcgatga ccgcgggcac
    19021 gaccgacggc gagacgacga agcgcgcgcc cgcgtcgaca gggcggtcga cgtccgctgc
    19081 ggtgagcacc gtgcccatgc ccaggtcgac tccggcgggg acccgcgccg caagccgttc
    19141 gacaacctcc agggcaccgc gtgtggtcat cgtcagctcg aggcagcgga tgccgccctc
    19201 caccagtgtg tcgaccacgg cctccgcgcg ggaggcgtgc tccgcgcgga ggatggcaac
    19261 gacccgggtg gtgaccagcg cctcactcat cggaacgggc ggcggcacga gtgctgcggc
    19321 tcgctgcccc ggaccatgga ggtaggtgct gccgagccgg gcgactgcgg cacccagccg
    19381 gtgccggccc gcggtcggct cgagctcgac gtagccgtgg tcgagcaacg gggcgaccag
    19441 ccgggcgacc gtgctcctgg ccagccccgc gtccgcggcc agctccgtca gtgtgcatcc
    19501 gccggcggcg tgacgggtgg cgaccacctc gatgaggctg agcgctctgc ggagggaagt
    19561 cgaggagttg cgcggcgccg cgcccggcat cgccgcctcc gtcccgttcg tcctgcaatg
    19621 cagaagttag gctgcattgg tgcgattgca tcatgcggga cgggttcgcc tggtacaaga
    19681 gcttcgcgta gtccgccgat cgacccgctc cggaccgcga cacgttcgcg ttcgcgcagg
    19741 tcgtcccgat gcgggcgggc cggagtcatt tgacggatgc cgcgccggag cccgtcgact
    19801 cacacttgcc gggcagtgag tgtgcccgtg cggaggaggc ccgtcatgac gatcgcgcag
    19861 tcggagagcg acaagatcta cgacgaactc atcgccgccc acacgatcat gcggcggggc
    19921 gtggatctgt tgcgcaccgc tctggcgcgc acaccgacgg ctcccgtcga ggtggacagc
    19981 tgtgtggggg tggggcgctg gctcatcgag ttcgtccacc agcaccagcg caccgaatcg
    20041 gacctgttct ggcagccgct ggccgatgcg tccccgaccg acgtcgggga gctccacgat
    20101 ctgacggtcc agcatatcca gatcgatcgg gaactggacc agctcgcctc gcgcctggac
    20161 cacctcgagg ccggccacga agccgccgcc gcccaggcac tgagagatct ggatgtcgta
    20221 cgccagaccc tctccgatca cctcgacgag gaggagtcac tcctcaaggc gctgttcccg
    20281 atgttggagc cg
//
LOCUS       DASRSC010000116         3884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2328960,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000116 DASRSC010000000
VERSION     DASRSC010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2328960"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="VFE39_05545"
     CDS             <1..209
                     /locus_tag="VFE39_05545"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031411 - gas vesicle [Evidence IEA];
                     GO_process: GO:0031412 - gas vesicle organization
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HET6258099.1"
                     /translation="VASRRYQPQDPRLSGRREEMVLNVAYLVDEAGADALRRLVEQHD
                     GQSLRLELTGPWAPYSFATLEEEP"
     gene            206..376
                     /locus_tag="VFE39_05550"
     CDS             206..376
                     /locus_tag="VFE39_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HET6258100.1"
                     /translation="MSVVPDRDVALVDLLDRLLNGGVVITGDITISLADVDLVHVSLR
                     ALISSVETLIEH"
     gene            428..724
                     /locus_tag="VFE39_05555"
     CDS             428..724
                     /locus_tag="VFE39_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010313744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein K"
                     /protein_id="HET6258101.1"
                     /translation="MAELPARLPLDPDAVGRDLGRLVLTIVELVRQLMERQAIRRVEA
                     GNLPDETVERLGLGLMRLEEAMTELREHFGLRAEDLNIDLGPLGSLLPRTNDRD"
     gene            complement(913..2043)
                     /gene="galK"
                     /locus_tag="VFE39_05560"
     CDS             complement(913..2043)
                     /gene="galK"
                     /locus_tag="VFE39_05560"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET6258102.1"
                     /translation="MTGFRELFGREADGRWAAPGRVNLIGEHVDYAQGLCLPFAIAER
                     TVVEVAARDDGRARLHSLAEQDDWSGTLDEVGPRSPDGWAGYAAGVLWALRQAGHEVG
                     GIDLLVTDSVPLGAGLSSSAALECSTALAVDELFGLGLGGTPSGRRALAAACISAEND
                     VVGAATGGMDQATSLLGVAGHALLLDMRDGTDRAVPFAPADDGLVVMVIDTRVRHRLA
                     DGQYGVRRSAVEKAAAALGMPSLRAATLAEVTTLDPDLVPRARHIVTEIARVRETVAL
                     LDAGRLSEIGPLLDASHASLAHDYEVSCDELDLACSAARRAGALGARMTGGGFGGSAI
                     ALVPVDRADAVASTVRAAFAEALYREPDIRGAQPSAGAERVG"
     gene            complement(2040..3188)
                     /gene="galT"
                     /locus_tag="VFE39_05565"
     CDS             complement(2040..3188)
                     /gene="galT"
                     /locus_tag="VFE39_05565"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HET6258103.1"
                     /translation="MSERASASSTSHRVRRTAARMADGRELFYFDDTEPYLSGDATRV
                     VPDTRDLPQVQHNSIMRFDVLTGEWVTIAAHRMDRTFLPPADHCPLDPTRPGREPTEV
                     PAPDYDVVVFENRFPSFAENIAADVAPEVDGNPLWPQRPAVGRCEVVLFTSDHNSSFA
                     ALPVERVRTVIEAWADRTAELSAMPGIDEVFPFENRGKEIGVTLEHPHGQIYAYPFVT
                     PRTAQLLARSAEHRQRTGRDLMGDVLDSERKAGSRVVLEGRHFTAFVPAAARWPVEAH
                     LVPHRAAPDIAALNGAERDELAVLYRDLLQRFNRYFGELPPYIAGWHQAPVHTGRDLT
                     RLHLRLFSLRRAPGKLKYLAGSESGVGAWINDASPERIAQRLRDVGPA"
     gene            complement(3185..>3884)
                     /locus_tag="VFE39_05570"
     CDS             complement(3185..>3884)
                     /locus_tag="VFE39_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET6258104.1"
                     /translation="ELVERLGVSDMTVRRDIDVLATRGLVSRVHGGATAAGSSVDEPG
                     FAAKSGLHTAAKQAIAAAAAALIEPGASVALSAGTTTHAVASALLTVPRLTVVTNSLR
                     VAEVLHESPRDDLTVVLTGGERTPSDALVGPVAVAALRRLHVDWLLMGVHGMDAEAGF
                     TTPNLVEAETNRALVASARRVAVVADNSKWGVVGLSTIATLDEVDVVVTDGGLEADAR
                     KILESSCARLVLAS"
BASE COUNT          527 a         1446 c         1385 g          526 t
ORIGIN      
        1 ccgtcgcgag ccggcgctac cagccgcagg acccgcgcct gtccggccgt cgcgaggaga
       61 tggtgctcaa cgtcgcgtac ctcgtggacg aggccggtgc ggacgccctg cgccggctgg
      121 tcgagcagca tgatgggcag agcctgcggc tggagctcac cggtccgtgg gcgccgtact
      181 ccttcgcgac cctggaggag gaaccgtgag cgtcgtgccc gaccgcgatg tggcgctggt
      241 cgacctgctc gaccggctgc tcaacggcgg tgtcgtgatc accggggaca tcaccatctc
      301 gctggccgat gtcgacctgg tgcacgtctc actccgtgcc ctgatcagct ccgtcgagac
      361 cctgatcgaa cactgatggt catcgaacac tgatggtcat cgaatactga ctggtggagg
      421 tggaacagtg gccgagctcc cggcacgcct gccgctcgac cccgatgccg tgggccgcga
      481 cctcggcagg ctcgtcctca ccatcgtgga actggtgcgc cagctgatgg agcgtcaggc
      541 gatccggcgg gtcgaggcgg gcaacctccc ggacgagacg gtcgagcggc tcgggctcgg
      601 gttgatgcgc ctcgaggagg cgatgaccga gctgcgggag cacttcgggc tgcgcgccga
      661 ggacctcaac atcgacctcg gccccctcgg gagcctgctc ccccggacaa acgaccgcga
      721 ctagcacccg cgccgcgagt cgcccgtttc cggcccgcga gtcgccggtt tcgcgcccgc
      781 gagtcgcccg tccccggccc gcgagtcgcc cgtttcgcgc ccgagccgcc cgtttcgtgc
      841 ctgcgagtcg ccgctacggg tgggcgtgtc gggccttctc cgctgttgag ccgcccgtcg
      901 gcccgctcgc cgtcagccca cccgctccgc ccccgccgac ggctgcgccc cgcggatgtc
      961 cggctcccgg tagagggcct ccgcgaacgc cgcccggacc gtcgacgcca cggcgtctgc
     1021 ccggtcgaca ggaaccagcg cgatcgccga gccgccgaac ccgccgccgg tcatccgcgc
     1081 gccgagcgcc ccggcccgcc gcgcggccga acaggccagg tcgagctcgt cgcaggacac
     1141 ctcgtagtcg tgcgcgagcg acgcatgcga cgcgtccagc agcggaccga tctcggacag
     1201 ccgccccgcg tcgagcagcg cgaccgtctc acggacacga gcgatctccg tgacgatgtg
     1261 gcgcgcccgt gggacgagat cagggtcgag cgtcgtgacc tcagcgagcg tggcggcgcg
     1321 gaggctgggc atcccgagtg ccgcggccgc cttctcgacg gccgagcggc gcaccccgta
     1381 ctgaccgtcg gcgagccgat gccgcacccg ggtgtcgatc accatgacga ccagcccgtc
     1441 gtccgcaggg gcgaacggga ccgcccggtc cgttccgtcg cgcatatcga gcaggagtgc
     1501 gtggccggcg acacccagca gtgacgtcgc ctggtccatc ccgccggtgg ccgcgccgac
     1561 cacgtcgttc tcggcgctga tgcaggctgc cgccagggca cggcgaccag acggggtgcc
     1621 gccgagcccc agcccgaaca gctcgtccac cgccagggcg gtggagcact ccagggccgc
     1681 cgacgaggag agcccggccc cgagcggcac cgagtcggtg accagcaggt cgatcccacc
     1741 gacctcgtgc ccggcctgcc gcagcgccca cagcacgccg gctgcgtagc cggcccagcc
     1801 gtcgggggag cgggggccga cctcgtcgag cgtgccgctc cagtcgtcct gctcggcgag
     1861 gctgtgcagc cgcgcgcggc cgtcgtcccg ggccgccacc tccaccaccg tgcgctcagc
     1921 gatcgcgaac gggaggcaaa gcccctgcgc atagtcgacg tgctcgccga tcaggttgac
     1981 gcgtcccggc gcggcccacc ggccgtcggc ctcgcgcccg aagagctccc ggaaccccgt
     2041 cacgccgggc ccacgtcccg cagccgctgc gcgatccgtt ccggcgaggc gtcgttgatc
     2101 caggcgccga cgcccgactc ggaccccgcg aggtacttga gcttcccggg tgcccggcgc
     2161 agcgagaaca gccgcaggtg cagccgggtg aggtcacgcc cggtgtgcac gggcgcctgg
     2221 tgccatccgg cgatgtaggg cgggagctcg ccgaagtagc ggttgaaccg ctgtagcagg
     2281 tcgcggtaca gcacggcgag ctcgtcgcgc tccgcgccgt tcagggcggc gatgtctggg
     2341 gcggcccggt gcgggacgag gtgtgcctcg acgggccagc gcgccgccgc gggcacgaac
     2401 gccgtgaagt gccggccttc gaggacgacg cggctgccgg ccttgcgctc gctgtcgagc
     2461 acgtcgccca tgaggtcgcg accggtgcgc tgccggtgtt ccgcggagcg cgcgagcagt
     2521 tgcgccgtgc gcggggtgac gaacggatag gcgtagatct gcccgtgcgg gtgttcgaga
     2581 gtaaccccga tctccttgcc gcggttctcg aagggaaaga cctcgtcgat ccccggcatc
     2641 gcggagagct cggcggtgcg gtccgcccac gcctcgatca cagtgcgcac ccgctccacg
     2701 ggcagcgcgg cgaaggagga gttgtggtcg ctcgtgaaca agacgacctc gcagcgcccc
     2761 acggcagggc gctggggcca cagcgggttg ccgtccacct cgggcgcgac gtccgcggcg
     2821 atgttctccg cgaaggaggg gaacctgttc tcgaacacga ccacgtcgta gtcgggcgcc
     2881 gggacctcgg tcggttcgcg gcccgggcgc gtggggtcca gcgggcagtg gtcggccggc
     2941 gggaggaagg tgcggtccat gcggtgcgcc gcgatcgtca cccactcccc ggtgagcacg
     3001 tcgaaccgca tgatcgagtt gtgctgcacc tgggggaggt cgcgggtgtc gggcaccacc
     3061 cgagtcgcgt cgccgctcag gtacggctcg gtgtcgtcga agtagaacag ctcgcgcccg
     3121 tcggccatcc gcgccgcggt gcgccggacc ctgtgcgacg tcgaggatgc gctcgcacgc
     3181 tcgctcatga cgccagtacc aaccgtgcgc aggagctctc gaggatcttg cgggcgtccg
     3241 cctccagccc gccgtccgtc accaccacgt ccacctcgtc gagagtcgcg atcgtgctca
     3301 ggcccaccac tccccatttc gagttgtcgg ccaccacggc gacgcggcgg gcgctcgcga
     3361 ccagcgcccg gttcgtctcg gcctccacca ggttcggcgt ggtgaacccg gcctcggcgt
     3421 ccatgccgtg cactcccatg agcagccagt cgacgtgcag tctgcgcagg gcggccaccg
     3481 cgaccggccc gaccagcgcg tccgacgggg tgcgctcccc gcccgtgagc accacggtca
     3541 gatcgtcgcg aggcgactcg tgcagcacct ccgccacgcg cagcgagttg gtcacgacgg
     3601 tcagccgcgg cacggtgagc agcgccgatg ccacggcgtg ggtagtggtg cccgccgaga
     3661 gcgccacgga cgcgcccggc tcgatcagcg cggccgccgc cgccgcgatg gcctgcttgg
     3721 ccgccgtgtg caggcccgac ttggcagcga accccggctc gtcgacgctg ctgccggccg
     3781 cggtcgcccc gccgtgcacg cgcgagacca gcccgcgggt cgcgagcacg tcgatgtcgc
     3841 ggcggacggt catgtcggag accccgagcc gttccacgag ttcg
//
LOCUS       DASRSC010000117         2298 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1182555,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000117 DASRSC010000000
VERSION     DASRSC010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182555"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VFE39_05575"
     CDS             complement(<1..195)
                     /locus_tag="VFE39_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258105.1"
                     /translation="MIDEHRRSSVPIAVQNISAVEVITAKRDGGRLSDEQIDWVVSAY
                     THGDVADEQMASLAMAILLNG"
     gene            353..544
                     /locus_tag="VFE39_05580"
     CDS             353..544
                     /locus_tag="VFE39_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258106.1"
                     /translation="MSRSIRDAECTRRRSTLDFFMNKVAPRANTVHRVNASHSPFLSR
                     PAELADLIGTIALTPARTA"
     gene            complement(604..1005)
                     /locus_tag="VFE39_05585"
     CDS             complement(604..1005)
                     /locus_tag="VFE39_05585"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET6258107.1"
                     /translation="MTEPNWDALREAAVAAAGSAYAPYSHLQVGAAAVCDDGRVVTGC
                     NVENASYGLGLCAECTMAGQLRLTGGGRFVAVACRSGAGELLMPCGRCRQILYEFGGP
                     DCLVDTPRGILPMREVLPDAFGPADLPDRRA"
     gene            complement(998..2176)
                     /locus_tag="VFE39_05590"
     CDS             complement(998..2176)
                     /locus_tag="VFE39_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005461604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6258108.1"
                     /translation="MIAAGVVILSVTATLTGQYQLTSRGTTQAAVLLLLPILFAGLGG
                     LWSERAGVINIGLEGMMILGTWGAAWAGYQWGPWAAVLGGIVLGALGGLLHAVATVTF
                     NVNHIVSGVAITLLGMGVTKYLSTLLFEPISQNPRQSPRVEAFATFSVQPVADGLASV
                     ESQQRVLLSDVAGLLGGLLSGLTPLVIIGFALVPISYLVLWRTRFGLRLRSCGENPVA
                     AESLGVKVVTYKYVGVVISGALAGLGGAALTLNPGQLGYLEGQTGGRGYIGLAAMIFG
                     NWRPGGLLAGSALFGYVDGVQLRAGGEAVHGLLYGATILAVGLAVVWAARRRWRAALI
                     AAIAGGLVYAIYYSIDTVPREFAAYAPQLVTLLVLAVASQRLRPPAAIGVEYRRGEGD
                     "
BASE COUNT          367 a          854 c          789 g          288 t
ORIGIN      
        1 gccgttgagc aggatggcca tcgcgagcga tgccatctgc tcgtcggcga cgtccccgtg
       61 cgtgtacgcc gagacgaccc agtcgatctg ctcgtcggac agtcgcccgc cgtcccgttt
      121 ggcggtgatg acctcgaccg cgctgatgtt ctggacggcg atgggcacgc tgctcctccg
      181 gtgctcgtcg atcacgacgc ctcaggatgc cagggacgac ccgcgggccg gattcgcggc
      241 tcgcagtcgg tactccccgt cggaaacgcc catcacgcgc ttgaacgcgg tgctgaacgc
      301 gctctcggat ccgtagcccg tcgtgccggc gatcgaggcg accgtacggc cggtgtcccg
      361 cagcatccgc gacgcggagt gcacccgcag gcggagcacc ctggacttct tcatgaacaa
      421 ggtggcgccg cgcgcgaaca ccgtccaccg cgtcaacgcc agccactcgc cgttcctgtc
      481 ccgaccggcc gagctggccg acctgatcgg cactatcgca ctcacgccgg ctcggactgc
      541 gtgacatcct cagaggggcg gcggccaccc agctcaccgc cggtccgggc agcgcagatg
      601 cgctcaggcc cgccggtccg ggaggtcggc gggcccgaat gcgtccggca gcacctcgcg
      661 catcgggagg atcccgcgcg gggtgtcgac gaggcagtca gggccgccga actcgtagag
      721 gatctgccgg cagcgaccgc acggcatcag cagctcaccg gcgccggacc ggcaggccac
      781 cgcgacgaac cggcccccgc ccgtgagccg cagctgcccc gccatcgtgc actccgcgca
      841 caggccgagc ccgtacgagg cgttctccac gttgcacccg gtgaccacac ggccgtcgtc
      901 gcacaccgcc gctgccccga cctgcaggtg cgagtagggc gcgtaggccg atccggccgc
      961 cgctaccgcg gcctcgcgca gcgcgtccca gttgggctca gtcaccttcg ccccgccggt
     1021 actcgacgcc gatggccgct gggggccgca gtcgttgtga cgccacggcg aggaccagca
     1081 gcgtgacgag ctgcggtgcg tacgccgcga actcgcgcgg cacggtgtcg atcgagtagt
     1141 agatggcgta caccaggcca cccgcgatcg cggcgatcaa cgccgcgcgc caccggcgcc
     1201 gcgccgccca caccaccgcg agcccgacgg cgaggatcgt cgccccgtag agcaggccat
     1261 gcacggcctc gccgccggcc cgcagctgga ctccgtcgac gtagccgaac agcgccgaac
     1321 cggcgagcag accaccggga cgccagttgc cgaagatcat cgcggcgagc ccgatgtacc
     1381 cgcggccgcc ggtctggccc tccaggtagc cgagctggcc ggggttgagc gtcagcgccg
     1441 ctccgcccag gccggcgagt gcgcccgaga tgaccacgcc gacgtacttg taggtgacga
     1501 ccttgacccc gagagactcg gcggccaccg ggttctcccc gcaggagcgc agccgcagcc
     1561 cgaagcgcgt gcgccacagc accaggtagc tgatcgggac gagcgcgaac ccgatgatca
     1621 cgagtggcgt gagcccgctg agcaggccgc cgagcaaccc cgccacgtcg gagagcagga
     1681 cacgctgctg ggactcgacg gacgccaggc cgtcggccac cggttgcacg gagaacgtgg
     1741 cgaacgcctc gacccgcggt gactggcgcg ggttctgcga gatcggctcg aacaacagcg
     1801 tcgacaggta cttcgtcacg cccatgccga ggagggtgat cgcgacgccg gacacgatgt
     1861 ggttgacgtt gaacgtcacg gtcgcgacgg cgtgcagcag cccgccgagc gcaccgagga
     1921 cgatgccacc gaggaccgcc gcccacggcc cccactggta accggcccag gctgcacccc
     1981 aggtgccgag gatcatcatg ccctcgaggc cgatgttgat gacccccgcg cgttcggacc
     2041 acagcccgcc gagaccggcg aacaggatcg ggagcagcag caggaccgcc gcctgcgtag
     2101 tgccccgcga ggtcagctgg tactgcccgg tcagggtggc ggtgaccgag aggatcacta
     2161 ctccggccgc gatcatgctc acgacacgca cccatgccgg gatgcggggc cgccgcggga
     2221 cgggcggggg agccgctgtc acgggtgctc tcgtcagggc cgtcatggcg ccaccttctc
     2281 cgcagctgtg gcgctgcg
//
LOCUS       DASRSC010000118         1613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1346957,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000118 DASRSC010000000
VERSION     DASRSC010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1346957"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1586)
                     /locus_tag="VFE39_05595"
     CDS             complement(21..1586)
                     /locus_tag="VFE39_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010311498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6258109.1"
                     /translation="MISSTARGIGSFIEGKWRDDAPGGRLASVDPARGDTVAEALLGD
                     AGTFVEACRVARDAQRGWAATPAPVRGRVVEQIGRLVEANAHALAALVTREIGKPLAE
                     SRGEVQEVIDTCTFFAGEGRRLYGQTVPSEMPDKQPFTFRVPVGVAAIVTAGNFPVAV
                     PSWYLVPALLCGNAVVWKPAEYTPAIAEAFTELFTRGGLPDGVLNTVLTDGPATFDGL
                     ATALDAGLVDKIGFTGSSAVGVRIGELAGRHLQNPCLELGGKNPLVVMPDADLDLAVE
                     GALFSGFGTAGQRCTSLGTVVVHESVHDEFLRRYEAAVRSAVIGDPTRDDVLYGPMIN
                     ARFLESFERHLDLVRPHHTVHGSTGTGRITADNPREGFAGDAAAGLYAHPTIVDGVRA
                     EDEIYRTETFGPIVGIAAFGTFDEAVELANGHGYGLSASIYTSTPVHAFRFRERVSAG
                     MVSVNNSTSGAEAHLPFGGNGRSGNGSRQSGVWVVDQFTRWQSMNWDYAGKLQKAQMD
                     TAQLPADVGFRLP"
BASE COUNT          224 a          586 c          578 g          225 t
ORIGIN      
        1 cgtatccgcg cacgaccggg ttaggggagg cggaagccca cgtcggcggg gagctgcgcg
       61 gtgtccatct gggccttctg cagcttgccc gcgtagtccc agttcatcga ctgccaccgg
      121 gtgaactggt cgaccaccca cacgcccgac tggcggctcc cgttcccgga gcgtccgttg
      181 ccgccgaacg gcaggtgcgc ctcggcgccc gaggtcgagt tgttgacgct caccatcccc
      241 gcgctcaccc gctcccggaa ccggaacgcg tgcaccggcg tggacgtgta gatcgaggcc
      301 gagagcccgt agccgtgccc gttggccagc tcgacggcct cgtcgaacgt cccgaacgcg
      361 gctatgccga cgatcgggcc gaacgtctcg gtgcggtaga tctcgtcctc ggcccgcacc
      421 ccgtcgacga tcgtcgggtg ggcgtagagg cccgcggccg cgtcaccggc gaagccctcc
      481 cgcgggttgt ccgcggtgat ccgtccggtg ccggtggaac cgtgcaccgt gtggtggggg
      541 cgcacgaggt cgaggtggcg ctcgaagctc tccaggaacc gggcgttgat catcggcccg
      601 tagaggacgt cgtcgcgggt cgggtcgccg atcaccgccg atcggacggc ggcctcgtag
      661 cggcgcagga actcgtcatg cacgctctcg tgcacgacga cggtgcccag agaggtgcag
      721 cgctgcccgg ccgtgccgaa gccggagaag agtgcgccct ccacggccag gtcgaggtcg
      781 gcgtccggca tgacgacgag cggattcttg ccgcccagct ccaggcacgg gttctgcagg
      841 tgccgcccgg cgagctcgcc gatccgcacg cccacagccg acgacccggt gaacccgatc
      901 ttgtcgacca ggccggcgtc gagcgccgtc gcgagcccgt cgaacgtggc gggcccgtcg
      961 gtcagcaccg tgttcagtac accgtcgggg aggccgccgc gcgtgaacag ctcggtgaac
     1021 gcctcggcga tggccggggt gtactcggca ggcttccaga cgaccgcgtt gccgcacagc
     1081 agcgcgggca cgaggtacca cgagggcacc gccaccggga agttcccggc cgtgacgatc
     1141 gctgcgaccc cgacgggcac gcggaacgtg aagggctgct tgtcgggcat ctcgctgggc
     1201 acggtctgtc cgtagagcct gcggccctcc ccggcgaaga aggtgcaggt gtcgatcacc
     1261 tcctgcacct cgcccctgga ctccgcgagc ggcttcccga tctcgcgggt gacgagcgcg
     1321 gccagcgcgt gggcgttggc ctcgacgagg cggccgatct gctcgaccac gcggccgcgg
     1381 accggcgccg gggtggccgc ccagccgcgc tgcgcgtccc gggcgacccg gcaggcctcg
     1441 acgaaggtgc cggcgtcgcc gagcagagcc tcggccacgg tgtcgccgcg cgccggatcc
     1501 accgacgcaa ggcgcccgcc cggggcatcg tcgcgccact tgccctcgat gaacgaaccg
     1561 ataccgcgcg cggtgctgct gatcacgcgg cctcctcgct gacgctcgtc agc
//
LOCUS       DASRSC010000119         3883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3313488,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000119 DASRSC010000000
VERSION     DASRSC010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3313488"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..506
                     /locus_tag="VFE39_05600"
     CDS             129..506
                     /locus_tag="VFE39_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010316149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258110.1"
                     /translation="MTVSTPAQPTSAQPALQPAPSDIVATVADRLGRDQVFGPPVASG
                     GTTLIPVARVRAGGGVGGMRPGVASTGAGVVARPLGAFAVGPDGKVAWHPAVDVNRIV
                     LGGQLALAAVLVVASRRLRRRRR"
     gene            complement(514..969)
                     /locus_tag="VFE39_05605"
     CDS             complement(514..969)
                     /locus_tag="VFE39_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6258111.1"
                     /translation="MKEQRRARAIAMSLDEVDAFLAEQRTCRVATVGRNGPHATPLWF
                     VWQGGALWLTSLSRSQRWTDLQNDPRVAVVVDAGEAYGELRGVELRGRVEVVGEVPRT
                     GEPVPDLDGPEQAFADRYTDGTIVRDGRHAWLRLVPEKITSWDFRKLDG"
     gene            complement(982..1359)
                     /locus_tag="VFE39_05610"
     CDS             complement(982..1359)
                     /locus_tag="VFE39_05610"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET6258112.1"
                     /translation="MLAAIGWRVGEQLDAVVCADEVAAGRPAPDMIERAMELTGVTVP
                     ARVLVAGDTVLDVRAGHAAGAGVVVAVLPERRRPTSSPPSTPPGSWTGCVSCPVSWPL
                     HPPVTRRPGSPSDPAGHGTARLR"
     gene            1453..1620
                     /locus_tag="VFE39_05615"
     CDS             1453..1620
                     /locus_tag="VFE39_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258113.1"
                     /translation="MCREFTSSRGPVRQALAGLRDDGLIGAVKAAGLSCWTPCRHSRS
                     TPFSRSPTGPS"
     gene            1566..2093
                     /locus_tag="VFE39_05620"
     CDS             1566..2093
                     /locus_tag="VFE39_05620"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HET6258114.1"
                     /translation="MPAQPFDTLLSFSHWAELMGRTPGQRTQQIALRRPEPAVAAALQ
                     LGPGARVVQLVRLRLLDGEPAMLERTTFVEPLGRTLLSVDLDAGSIYAMLTACGVDLH
                     AARHTFDAVAAERQDAVLLEVAEGAPLLRERRLTTSSTGEPLEWSDDRYRPDIATVTI
                     SNTRRSRPGMARTWP"
     gene            2131..>3883
                     /locus_tag="VFE39_05625"
     CDS             2131..>3883
                     /locus_tag="VFE39_05625"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HET6258115.1"
                     /translation="MADESTTSDLIVERLLEWGVDICFGICGDQVNGFFEAIRTRADR
                     FRFVHVRHEESAALAAVGHAKFTGRPAAVVTTAGPGALHLLNGLYDARMDGAPVIVIT
                     GIPHHDVLGTHMLQDVPSDRLFEQVCPFNERVMGRAHAAAMTDLAVRSALQNRTPSHL
                     AIPLDVQSMTEDTPSPKNVPDHLSTAWSPATLTPPADLVHAAADLLNSCERVAICAGA
                     GARGAGDVLEVIADSLGAPIVKAGLGKAVVPDDSPFTTGGMGLLGTRASQEVFEQCNG
                     FLIVGSATPYAEFWPKPGQARTVQIDVNADRIGLRHPVEIALVGHAEPTLTALLPLVH
                     ERRDRSFLEQAQRGVLRWWDLLREQANSSGSPIRPQVVTVALSELLPDRAIVTGDAGS
                     NTFVSHRLRLRRGMDYGFSGTLCTMGSAVPYAIGAQIAFPQRPVVAVVGDGGMAMGMG
                     ELATLAQYQLPITVVVLRNNALALEIWEQAALLGNPQYGCELSPVDFAGVARACGLEG
                     WSVSEPGDVRAALAAALAHDGPSLVEVVVDPFEAPFGETLKPEQAQKIVQAFDKGEPA
                     AGAMARNLLDPARRVLSP"
BASE COUNT          513 a         1409 c         1402 g          559 t
ORIGIN      
        1 tagaggaacg gcgtgcgggg accctcggcg atcacgtcgt gctgacctgg cttgatccgc
       61 gagtctcgcc gaaggccggc gggatcctgc cgtagccagg ggtgctgaag aacggcaggc
      121 tggacgatat gaccgtatcg actccggccc agccgacgtc cgcccagccg gctctccaac
      181 ccgcgccgtc ggacatcgtg gccaccgtcg ccgaccggct cggccgcgac caggtgttcg
      241 gtccaccggt cgcgagcggc gggaccacgc tgattccggt ggcgcgggtg cgggcgggcg
      301 gcggggtggg cggcatgcgt cccggggtgg cgagcaccgg ggccggcgtc gtggccaggc
      361 cactcggtgc cttcgccgta ggcccggacg ggaaggtggc ctggcacccg gccgtcgacg
      421 tcaaccggat cgtcctgggt ggacagctgg ccctcgccgc cgtcctcgtg gtggcgtcgc
      481 gccggctccg ccgacggcgc cggtagccgc agctcagccg tcgagcttgc ggaagtccca
      541 gctggtgatc ttctccggca ccaggcgcag ccaggcgtgg cgtccgtcgc ggacgatggt
      601 gccgtcggtg tagcggtcgg cgaaggcctg ctccgggccg tccaggtcgg gtaccggttc
      661 gcccgtgcgc ggcacctcgc cgacgacctc gacccgcccg cgcagctcca ccccgcgcag
      721 ctcgccatag gcctctccgg cgtcgacgac gacggcgacc ctcgggtcgt tctgcaggtc
      781 tgtccagcgc tggctgcgcg acagcgaggt cagccacagc gctccgccct gccagacgaa
      841 ccacaacggg gtggcgtgcg ggccgttccg gccgacggtg gccacccggc aggtgcgctg
      901 ctccgcgagg aaggcgtcga cctcgtcgag ggacatcgcg atcgcgcggg cgcgccgttg
      961 ctccttcata ggcgttggac gctagcgcag ccgtgccgtg ccgtggccgg ccggatccga
     1021 tggactaccg ggccgtcggg tcaccggcgg gtgcagcggc caggagaccg ggcagctcac
     1081 gcaccccgtc caggagccgg gtggggtact cggcggcgag ctcgtcggcc gtctgcgctc
     1141 cggtagcacc gcgacgacga cgcctgctcc cgccgcgtgc ccggcgcgga cgtcgagcac
     1201 ggtgtcgccg gccacgagga cacgcgcggg cacggtcacg cccgtcagct ccatcgcgcg
     1261 ttcgatcatg tccggcgccg ggcgtcccgc cgccacctcg tcggcgcaga ccaccgcgtc
     1321 gagctgctcg ccgacccgcc aaccgatcgc ggcgagcaac ggatcggtga cctgctggtc
     1381 gaacccggtg gtcagggcga catccgctcc ggagcgcttc cggtcggcgc gcccgtcccg
     1441 tccgaggcgg agttgtgccg cgagttcacc tcgtcccgcg ggccggtacg gcaggcgctg
     1501 gccgggctgc gcgacgacgg gttgatcggg gcggtcaagg ccgccgggct gtcgtgctgg
     1561 acgccgtgcc ggcacagccg ttcgacaccc ttctctcgtt ctcccactgg gccgagctga
     1621 tgggccggac gcccggacag cgcacgcagc agatcgcgct gcgcaggcct gaacccgctg
     1681 tcgctgcggc cctgcagctg ggccccggcg cccgggtcgt gcagctggtg cggcttcggc
     1741 tgctggacgg cgagccggcc atgctggaac gcacgacgtt cgtcgagccg ctgggcagga
     1801 cgttgctctc cgtcgacctg gatgccggat cgatctacgc catgctcacc gcctgcggag
     1861 tcgacctgca cgcagcccgg cacaccttcg atgccgtggc cgccgagcgg caggacgcgg
     1921 tcctgctgga ggtcgccgag ggcgctccgc tgctgcgaga gcgccgcctg accaccagct
     1981 cgaccggtga gccactggag tggtccgacg accgctaccg gcccgacatc gcgaccgtga
     2041 cgatctccaa cacccgccgg tcgcgacccg ggatggcccg cacctggcca tagatgcggg
     2101 tcgcccgccc ccagatgggt accagcaggc atggcggacg agagcaccac ctcggacctg
     2161 atcgtcgagc ggctgctcga atggggcgtc gacatctgct tcggtatctg tggcgaccag
     2221 gtcaacggat tcttcgaggc gatccgcaca cgcgcggacc ggttccgctt cgtgcacgtg
     2281 cgccacgagg agtcggccgc gctcgcggcg gtgggccacg ccaagttcac cggccggccg
     2341 gccgcggtgg tgacgaccgc ggggccgggt gcgttgcacc tactcaacgg cctctacgac
     2401 gcccggatgg acggtgcccc cgtcatcgtg atcaccggga tcccgcacca cgacgttctc
     2461 ggcacgcaca tgctccagga cgtgccgtcc gatcgtctgt tcgagcaggt gtgccccttc
     2521 aacgagcgcg tgatgggccg cgcgcacgcc gcggcgatga ccgatctcgc tgtgcgcagc
     2581 gcactgcaga accggacacc gtcgcacctc gcgatcccgc tggacgtcca gtcgatgacc
     2641 gaggacaccc cgtcccccaa gaacgtgccg gaccacctct cgacagcgtg gtcccccgcc
     2701 acgctcacgc cgcccgcgga cctcgtccat gccgccgccg acctcctgaa ctcctgcgaa
     2761 cgcgtcgcga tctgcgcggg tgccggagcg cgcggcgcag gcgacgtgct cgaggtgatc
     2821 gccgacagcc tcggcgcgcc gatcgtcaag gccgggctcg gtaaggccgt ggtgcccgac
     2881 gacagcccct tcacgaccgg cggcatgggc ttgctcggca cgcgggccag ccaggaggtc
     2941 ttcgagcagt gcaacggctt cctgatcgtc ggctcggcca cgccctacgc ggagttctgg
     3001 ccgaagcctg gacaggcgcg gacggtgcag atcgacgtca acgccgaccg gatcgggctg
     3061 cgccacccgg tcgagatcgc actggtcgga cacgccgagc cgaccctcac ggcgttgctg
     3121 cccctggtcc atgagcgccg cgaccgttcc ttcctcgagc aggcgcagcg tggtgtcctg
     3181 cgctggtggg atttgctgcg ggagcaggcg aacagctcag ggagcccgat ccggccgcag
     3241 gtggtgaccg tcgcgctgtc ggaactgctg cccgaccgtg cgatcgtcac cggcgatgct
     3301 ggcagcaaca cgttcgtctc ccaccggctg cggctgcgtc gtgggatgga ctacggcttc
     3361 tccggcacgc tctgcacgat gggctcggcg gtgccgtacg cgatcggggc gcagatcgcg
     3421 ttcccgcagc ggccggttgt cgcggtggtc ggcgacgggg gcatggccat gggcatgggg
     3481 gagctcgcca cactcgcgca gtaccaactg ccgatcacgg tggtcgtgct gcgcaacaac
     3541 gcgctcgcgc tggagatctg ggaacaggcc gccctgctgg gcaacccgca gtacggctgc
     3601 gagctgtcgc cggtcgactt cgccggggtc gcgcgggcgt gcggtctcga ggggtggagc
     3661 gtcagcgagc ccggcgacgt ccgtgcggcc ctggccgctg cgctcgctca tgacgggccg
     3721 tccctcgtgg aggtcgtcgt cgacccgttc gaggcgccgt tcggggagac gctgaagccg
     3781 gagcaggcgc agaagatcgt tcaggcgttc gacaagggcg agcccgcggc cggggcgatg
     3841 gcgcgcaacc tcctcgaccc cgcccgccgc gtgctgtcgc ccg
//
LOCUS       DASRSC010000120         7514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1513176,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000120 DASRSC010000000
VERSION     DASRSC010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7514
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513176"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1124
                     /locus_tag="VFE39_05630"
     CDS             141..1124
                     /locus_tag="VFE39_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HET6258116.1"
                     /translation="MIVVVIVVALVFDYTNGFHDAANAIATSVATRALAPRTALAMAA
                     VMNLVGAFLGTEVASTVGSGIIEAPQGSSGLLVVLAALVGAIAWNLITWWLGLPSSSS
                     HALIGGLVGAALASSGGVQWGGVLEKVVIPMVVSPVVGFLLAGLVMVAILWLFRRGRP
                     RRLYRGFRHAQTVSAAAMALGHGLQDAQKTMGVIVLALVVGGYHEGFDVPWWVVLLSA
                     GALSAGTYTGGWRIMRTLGRRIIDLDPPRGFAAEVTASAVLYTAAFVFAAPISTTQTI
                     TSAILGVGATKRLSAVRWGVAGNIALAWILTIPMAALAAALAFWALRPLAA"
     gene            complement(1141..1986)
                     /locus_tag="VFE39_05635"
     CDS             complement(1141..1986)
                     /locus_tag="VFE39_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HET6258117.1"
                     /translation="MGKSVRETFRVPAQPDLSALDPRGRPIGPKDKSAAAEGIADLGA
                     RLDRLQEALYAEATGGGRSALLLVLQGMDTSGKGGVIRHVGGLVNPMGLHIASFKKPT
                     PEELQHHFLWRVRRQVPRPGLIGLFDRSHYEDVLVPRVEGVISEDALRHRYEEINAFE
                     AELVTQGVTVMKCMLHISPQEQAERLLARLDDPTKRWKYNPGDLDVRSKWREYQQAYT
                     DALRHCDSDAAPWYVVPSDRKWYRNWAVASLLAETLTELDPQFPQPNIDVDAERARLA
                     ASKIG"
     gene            complement(1996..2661)
                     /locus_tag="VFE39_05640"
     CDS             complement(1996..2661)
                     /locus_tag="VFE39_05640"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HET6258118.1"
                     /translation="MDDSVFDHEGPWTEGAFLALPRDGHVEVVDGTLLIGPIASEQRA
                     RVVGRLRAAVQSALPDGLRVQGPVPLRLGTDCVLVPDLVVTAAGDGPEAADAEDEPDE
                     VLEAGAALMVIEVVGCDHGATDRTFKPQLYARSRIPYSLVVDHDAPFAVADMIISGRY
                     YEYSRAGAGERLRIDEPFVLEFDLMTVVDPEAETGPTESSSGQAASPAGTEAPASTGA
                     WPH"
     gene            complement(2728..2853)
                     /locus_tag="VFE39_05645"
                     /pseudo
     CDS             complement(2728..2853)
                     /locus_tag="VFE39_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458618.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(2924..3004)
                     /locus_tag="VFE39_05650"
                     /pseudo
     CDS             complement(2924..3004)
                     /locus_tag="VFE39_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3094..3819
                     /locus_tag="VFE39_05655"
     CDS             3094..3819
                     /locus_tag="VFE39_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine reductase family protein"
                     /protein_id="HET6258119.1"
                     /translation="MHPLVPSPGGPLDDAGLAAHYAYPEGLAAPYVRVNFVTSADGAV
                     TVDGRSGGLGSAADRQVFGLLRELADVVLAGAGTVRAENYRGARRPTRGTDTPPPIAV
                     VSGSADLDPASRLFTDTAVPPIVLTLDSAPLDRRERLAAAGGDVVVLDRLTPDLVLAE
                     LARRGLHRVLCEGGPALFGSLVAADAVDELCLTVAPLLAGGTAGRIIRGPEGGHPRPM
                     DLVGVLEDAGILLLRYRRAAPIG"
     gene            3896..4378
                     /locus_tag="VFE39_05660"
     CDS             3896..4378
                     /locus_tag="VFE39_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET6258120.1"
                     /translation="MSETTSAPTVKSPTTSASSPARLADDTSQGRTTIAASVVQKIAG
                     IAAREISGVYSMGGGVSRAFGAIRERIPGGGTGGTNIAGVQVEVGEKQAAVDLDIVVE
                     YGASIVELARAVRRNVITAVERMTGLEVIEVNIAVNDIHLPDADNDEEIPTVQAARVE
                     "
     gene            4422..4748
                     /locus_tag="VFE39_05665"
     CDS             4422..4748
                     /locus_tag="VFE39_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258121.1"
                     /translation="MTVPTAAEADSLAERIAAVVTAHPAVLRLHGGVFGDVVTYLPGR
                     RLTGVRVGMGDEPVELGVVLVLDRPIPEVVRALRHEVSRMCSGAAVDITVADVALPGE
                     TGPTGP"
     gene            4745..5005
                     /locus_tag="VFE39_05670"
     CDS             4745..5005
                     /locus_tag="VFE39_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258122.1"
                     /translation="MNEQERSAFRAFVREHHPDRGGDPEVFVAGLARFGVAEPVRSDS
                     DDDHGDDRYDAPVEVVTPLPFPVRVGVALIRTWHRRHNRRVE"
     gene            5027..5206
                     /locus_tag="VFE39_05675"
     CDS             5027..5206
                     /locus_tag="VFE39_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258123.1"
                     /translation="MNSTQTGLLAGLILGLAAAFGGFGVFLIVLVMGAIGLIIGRVID
                     GQLDLNAVLGRGRDR"
     gene            5203..5580
                     /locus_tag="VFE39_05680"
     CDS             5203..5580
                     /locus_tag="VFE39_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET6258124.1"
                     /translation="MTASRLPAVDDRGVLEISPTVLRKIVEHAADQVPGTLRHERRLA
                     GIDVGEAGASAKVTPGSGDPSAVDVRLELHLQYPASVRSVVDAVRAKVGEELDRIAGH
                     RVRALTVTVAGLRGARATASRVQ"
     gene            5641..6180
                     /locus_tag="VFE39_05685"
     CDS             5641..6180
                     /locus_tag="VFE39_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6286 domain-containing protein"
                     /protein_id="HET6258125.1"
                     /translation="MRMLLRLLAPLIGLAMAAAGVLIVIEVVAGWARSNVSRGLVVPW
                     HRWRAAIENLSWDQPPVAGVAIAVAVVGLLLVLVGLLARRSVITIDGPAPEITVTTSP
                     RVVARLVGRRVRAIGDIFSASVTASPRKVSVVAQVWGEADPELRSAVRARVDELLDEL
                     PLHRRPRVAVSVQERKGAR"
     gene            6177..6836
                     /locus_tag="VFE39_05690"
     CDS             6177..6836
                     /locus_tag="VFE39_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET6258126.1"
                     /translation="MTTPVAHGSGTAPTDRRTATLRKRARSAVARSAAVDRVLAVVIG
                     LVLLAAGILVALLAYGVFGSARAGRPLLDPLIVDAFRAQPDVYRLVAIACGVLLVLVG
                     LTWAARSLRPERRPDVVLDGGPDTAIVVSSSAAADAVAAQAATLPGVGRARARMVGTD
                     EAPALRITLWLADDADVREVLARLHDEVLAAACASLGLAALPVAVRLELDPSGAPPRV
                     S"
     gene            6921..>7514
                     /locus_tag="VFE39_05695"
     CDS             6921..>7514
                     /locus_tag="VFE39_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258127.1"
                     /translation="MARAPRHAVAAAIVCLAMLVAGCTVGPSQRPPVAVRGQDMPVIP
                     TSPAAAVPDPAEPLPEPQDQRASIPFSECTEDTLSTLSTPLPTDRTLRVECGELTVPT
                     DPDQPGLGAATLSVLRVGLATAPENRPPLLVLGDTTLEPSARNAVMLAEQVVPEVLEN
                     YTLIGLDRRGAGEDNLDCADDDARAALVDADPDGSTEA"
BASE COUNT          963 a         2787 c         2667 g         1097 t
ORIGIN      
        1 acacacctcc cggcgacgtg ctcacgatcc tgaagatcaa ggaggtcgtc gaggtactcg
       61 aggaggccgc ggacgcgttc gagacggtgg ccaacaccgt cgagagcatc gtggtcaagg
      121 agtcctgagt ggagctcgcc atgatcgtcg tggtgatcgt cgttgcgctc gtcttcgact
      181 acaccaacgg ctttcacgac gccgccaacg cgatcgccac ctccgttgcc acccgcgccc
      241 tcgcgccccg caccgccctc gccatggcag cagtgatgaa cctggtcgga gcgttcctcg
      301 gcaccgaggt ggcctcgacg gtcggcagcg gcatcatcga ggcgccgcag ggctcgtccg
      361 gactgctggt ggtgctggcc gcactcgtcg gggccatcgc gtggaacctc atcacatggt
      421 ggctgggcct gccgtcctcg tcgtcgcacg cgctgatcgg tggcctcgtc ggggcggcgc
      481 tcgcgtcgtc cggcggcgtg caatggggcg gggtgctcga gaaggtcgtc atcccgatgg
      541 tggtatcgcc ggtggtgggg ttcctcctgg ccggtctcgt gatggtggcg atcctctggc
      601 tcttccgccg gggcaggccg aggcggctct accgtggatt ccggcacgcg cagacggtgt
      661 cggccgcggc aatggcactc ggccacggcc tgcaggatgc ccagaagacg atgggcgtga
      721 tcgtgctggc cctggtggtc ggcggttacc acgagggatt cgacgtcccg tggtgggtcg
      781 tcctgctctc ggcgggagcc ctgtcggccg gcacctacac cgggggctgg cggatcatgc
      841 ggacgttggg caggcggatc atcgacctcg atccgccgcg cggcttcgcc gccgaggtca
      901 ccgcgtcggc ggtgctctac acggccgcgt tcgtcttcgc cgcgccgatc tcgaccacac
      961 agacgatcac gtcggccatt ctcggggtag gtgccacgaa acggctctcg gccgtgcgct
     1021 ggggcgttgc cggcaacatc gccctcgcct ggatactcac gatcccgatg gcggcactcg
     1081 ccgccgcgct ggcgttctgg gcactgcgcc cgctggccgc ttgaccacgc ggccggtagc
     1141 tcagccgatc ttgctcgcgg cgaggcgcgc ccgctcggcg tcgacgtcga tgttcggctg
     1201 cggaaactgc gggtccagct cggtgagggt ctccgcgagc agcgaggcga ccgcccagtt
     1261 gcggtaccac ttgcggtccg acggcacgac gtaccagggc gctgcgtcgg agtcgcaatg
     1321 ccgcagggcg tcggtgtagg cctgctggta ctcgcgccac ttcgagcgga cgtccaggtc
     1381 gccggggttg tacttccagc gtttggtcgg gtcgtccagc cgggcgagca accgctcggc
     1441 ctgctcctgc ggcgagatgt gcaacatgca cttcatgacg gtcacgccct gcgtgacgag
     1501 ctccgcctcg aaggcgttga tctcctcgta gcggtggcgc aaggcgtcct cggatatcac
     1561 gccctcgacc cgtggcacga ggacgtcctc gtagtgcgag cggtcgaaca gcccgatcaa
     1621 ccccggacgg ggcacctgcc ggcggacgcg ccagaggaaa tggtgctgga gctcttcggg
     1681 cgtcggcttc ttgaacgacg cgatgtgcag gcccatcggg ttcaccaggc cgccgacgtg
     1741 cctgatcacg ccgcccttgc cggaggtgtc catcccctgc agcacgagca gcagcgcgga
     1801 ccgtcccccg ccggtcgcct ccgcgtacag cgcctcctgc aggcggtcga gacgcgcacc
     1861 gagatccgcg atgccctccg cagccgcgct cttgtccttc ggtccgatcg ggcgaccgcg
     1921 tgggtccagt gccgagaggt cgggctgggc cggcacccgg aacgtctcgc gcacggattt
     1981 ccccatccgg cgagactagt gcggccacgc ccccgtgctc gccggcgctt cggtgcccgc
     2041 tggtgatgcc gcctgccccg agctcgactc cgtcggtccc gtctccgcct ccgggtcgac
     2101 caccgtcatc aggtcgaact ccaggacgaa cggctcgtcg atgcggagcc tctcccccgc
     2161 tcccgcacgc gagtactcgt agtaccgacc actgatgatc atgtcggcga cggcgaacgg
     2221 ggcgtcgtgg tcgaccacca gcgagtacgg gatgcgactg cgcgcgtaga gctggggctt
     2281 gaaggtgcgg tcggtggcgc cgtggtcgca ccccaccacc tcgatcacca tcagcgccgc
     2341 gccggcctcg agcacctcgt ccggctcgtc ctccgcatcc gcggcctccg gcccgtcgcc
     2401 ggccgcggtg accaccaggt cgggcaccag cacgcagtcc gtgccgaggc gcagcggcac
     2461 ggggccctgc acccgcagtc cgtcgggcag cgccgactgc accgcggcac gcagtcgccc
     2521 caccacccgc gcgcgctgct cacttgcgat aggcccgatg agcagcgttc cgtccaccac
     2581 ctcgacatgg ccgtcgcggg gcagcgcgag gaacgcgccc tccgtccacg gcccctcgtg
     2641 gtcgaacacg gaatcgtcca ctgccgccgc ctctcggtcg tcgcccggtg ctcgggcgca
     2701 catcgtgaca cgcgcgagtc gcgagcatca tgacgaccgt gcgtgaccga cgatcggcac
     2761 gacgagcggg gtgccggcca ccgggtccgg gatcacgcgc gcggcgaggc cgaacacctc
     2821 ggcgagcagc gcctccgtga tcacctcggc cggttcgcgg gacgccggcg ctgagcaggc
     2881 cgccggtcag ggcggccagc ggcagcccgg tggctgcgag gaaccagccg agggtgcccg
     2941 acccgccgcc gatcacgatc acgaacaccg ccgcggcgct cgccccggcc gtgaagccga
     3001 tcatgtccgg gctcgcgagc gggttgcgcg cgatggactg ggtgatggcg cgccggacgc
     3061 caccaggcgc acatgccgtg ctgggatggc ggcgtgcatc ccctcgtgcc gtccccgggc
     3121 ggcccgctcg acgacgccgg gctcgcggcg cactacgcct accccgaggg gctcgcggcg
     3181 ccgtatgtgc gggtgaactt cgtgacgagc gcggacggcg ccgtcacggt ggacggccgg
     3241 tcgggcggcc tgggctcggc cgccgaccgc caggtgttcg ggctgctccg cgagctcgcg
     3301 gacgtcgtgc tcgccggggc cgggacggtg cgcgcggaga actaccgcgg tgcgcgcagg
     3361 ccgacccgcg gcacggacac cccgccaccc atcgccgtcg tgtcaggatc ggccgacctc
     3421 gatccggcga gcaggctctt caccgacacc gccgtgccgc cgatcgtcct cacgctggac
     3481 tcggccccgc tcgaccgccg ggaacggctc gccgcggcag gcggcgacgt ggtcgtgctg
     3541 gaccggctca cccccgatct cgtgctggcc gagctggctc ggcgcggcct gcaccgggtg
     3601 ctctgcgagg gcggtccggc cctgttcggc agcctggtcg ccgccgacgc ggtggacgag
     3661 ctctgcctca ccgtcgcgcc gctgctcgcc ggcggcaccg cggggcgcat catccgcggc
     3721 cccgagggcg gccatccccg tccgatggac ctggtgggag tgctggagga cgccggcatc
     3781 ctcctgctcc gataccgccg cgctgccccg atcgggtgac ttcccgtgag gcacgcccgg
     3841 gtatccccgt cggacgggcg aacactccgc acgagcgagc acggaggaat cttcgatgag
     3901 cgagacaacg tcagccccca ctgtcaagtc acccaccacc agcgcgtcct cgccggcccg
     3961 gctggccgac gacacctcgc agggccggac caccatcgcc gcctctgtcg tccagaagat
     4021 cgccggcatc gcggcccgcg agatctcggg cgtgtactcg atgggcggcg gtgtgtcccg
     4081 cgccttcggt gcgatccgcg agcggatccc gggcggcggt accggtggca ccaacatcgc
     4141 aggcgtgcag gtcgaggtcg gtgagaagca ggcggccgtc gacctcgaca tcgtcgtcga
     4201 gtacggcgcg tcgatcgtcg agctggcccg cgcggtgcgg cggaacgtga tcaccgccgt
     4261 cgagcggatg acgggtctcg aggtgatcga ggtcaacatc gcggtgaacg atatccacct
     4321 ccccgacgcc gacaacgacg aggagatccc gacggtccag gccgcgcgcg tggagtgagc
     4381 gcggcccgac cgcccggatc agttggcagg gagacgtgct cgtgacagtc ccgaccgccg
     4441 ccgaggcgga ctcgctcgcc gagcggatcg ccgcggtcgt gaccgcgcac cccgcggtcc
     4501 tccggctgca cggtggggtg ttcggcgacg tcgtcaccta cctgccgggc cgtaggctca
     4561 cgggcgtgcg cgtcggcatg ggtgacgagc cggtcgagct gggtgtcgtg ctcgtcctcg
     4621 accggccgat cccggaggtc gtgcgggccc tgcgccacga ggtgtcccgg atgtgcagtg
     4681 gcgccgcggt cgacatcacc gtggccgacg tcgcccttcc cggcgagacg gggccgacgg
     4741 gcccgtgaac gagcaggagc gctcggcatt ccgcgcgttc gtccgcgagc accacccgga
     4801 ccggggcggc gacccggagg tcttcgtggc agggctcgcc cggttcggcg tcgcggagcc
     4861 ggtgcggtcg gattcggacg atgatcacgg cgacgaccgt tacgacgcac ccgtcgaggt
     4921 ggtcacgcca ctgccgttcc ccgtccgggt cggggtcgcg ctgatccgca cctggcaccg
     4981 ccgccacaac cgccgggtgg agtagcaccc tcatgaggag aaccagatga acagcaccca
     5041 gaccggcctg ctggcgggcc tcatcctcgg ccttgcagca gccttcggtg gcttcggggt
     5101 cttcctgatc gtcctggtca tgggcgcgat cgggctcatc atcggccgcg tgatcgacgg
     5161 tcagctcgac ctcaacgcgg tgctcggacg gggtcgggac cggtgaccgc ttcgcgcctc
     5221 ccggcagtcg acgaccgggg cgtgctcgag atcagcccca ccgtgctgcg caagatcgtc
     5281 gagcatgccg ccgaccaggt gcccgggacc ctgcgccacg agcgtcggct cgccgggatc
     5341 gacgtcggcg aggccggggc cagcgcgaag gtcacgcccg gatccggtga tccgtccgcg
     5401 gtggacgttc gactcgagct ccacctgcag tacccggcgt ccgtgcgcag tgtcgtggac
     5461 gcagtgcgcg ccaaggtggg cgaggagctc gaccgcatcg ccgggcatcg cgtccgggcc
     5521 ctgacggtga ccgtcgccgg cctgcgcggc gcccgcgcca ccgcgtcccg ggtccagtag
     5581 ccggcccgtc gactggctcg ccgtcagccc cccacccatc gcatcggaca ggaggacgac
     5641 atgcgcatgc tgctccgcct gctcgccccg ctgatcggcc tggccatggc cgcagccggg
     5701 gtgctcatcg tgatcgaggt cgtcgccggg tgggcgcgtt cgaacgtgtc cagagggctc
     5761 gtcgtgccgt ggcaccggtg gcgcgccgcc atcgagaacc tgtcgtggga ccagccgccg
     5821 gtcgccggcg tcgcgatcgc cgtcgccgtc gtcggtctcc tgctcgtgct ggtcgggctg
     5881 ctcgcccgac gctccgtcat caccatcgac ggacccgcac cggagatcac cgtcaccacc
     5941 tccccgcgcg tggtggcccg gctggtgggc cggcgcgtcc gcgccatcgg agacatcttc
     6001 agcgcctcgg tcaccgcctc gccgcggaag gtgtccgtgg tcgcccaggt ctggggcgag
     6061 gctgatccgg agctgcgcag tgctgtgcgc gcccgcgtgg acgagctgct cgacgagctg
     6121 cccctgcacc gccgcccgcg ggtggccgtc tccgtgcagg aacggaaggg agcccgatga
     6181 ccacgcctgt agcccacggc agcgggaccg ccccgaccga ccgccggacc gcgacgctgc
     6241 ggaagcgggc ccgctcggcc gtggcccggt ccgcggctgt cgaccgcgtg ctcgcggtgg
     6301 tgatcgggct cgtgctgctc gccgccggca tcctcgtcgc gctactggcc tacggtgtct
     6361 tcggcagcgc acgggccggg cggccgctgc tggatccgct gatcgtcgac gcgtttcgcg
     6421 cccaacccga cgtctaccgc ctggtggcca tcgcctgcgg agtgctgctc gtcctcgtcg
     6481 ggctgacgtg ggccgcgcgg tcgctgcgcc cggaacggcg gccggacgtc gtgctcgacg
     6541 gtggccccga caccgcgatc gtcgtcagct cctccgcagc ggccgacgcc gtcgccgctc
     6601 aggcggccac cctccccggc gtgggccggg ccagggcgcg catggtcggc acggacgagg
     6661 cgcccgccct gcgcatcacg ctctggctgg ccgatgacgc cgacgtccgc gaggtgctcg
     6721 cccggctgca cgacgaggtc ctcgcggccg cttgtgcctc gctggggctc gccgcgctac
     6781 ccgttgccgt gcggctggag ctcgatccct caggggcgcc accccgagtg agctgacggc
     6841 agcgtgcacg ccccctgttc accctcccca caccggcggt gcggcagggt gggcgccgac
     6901 cagcaggatc gggagggcac gtggcacgag caccgcgtca cgcggtcgcc gccgcgatcg
     6961 tctgcctcgc catgttggtc gcgggctgca ccgtcggccc gtcccagcgc ccgccggtgg
     7021 cggtgcgcgg gcaggacatg ccggtcatcc cgacgtcgcc agctgcggcg gtgccggatc
     7081 ccgccgagcc gctccccgag ccgcaggacc agcgggcctc gatcccgttc tccgaatgca
     7141 ccgaggacac gctgtcgacg ctgtccacac cgctccccac cgaccgcacg ctacgggtgg
     7201 agtgcggcga gctcaccgtg cccaccgacc ctgaccagcc gggtctcggc gcggccacgc
     7261 tcagcgtgct gcgcgtcggc ctcgccacag ccccggagaa ccggccgccg ttgctcgtgc
     7321 tcggcgacac cacgctcgag ccgtcggccc gcaacgccgt catgctggcc gagcaggtcg
     7381 tgccggaggt cctcgagaac tacacgctga tcggcctgga ccgccggggc gcaggcgagg
     7441 acaacctcga ctgcgccgac gacgacgccc gcgccgcgct cgtcgacgcc gatcccgacg
     7501 ggtcgacgga ggcc
//
LOCUS       DASRSC010000121         7351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4789126,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000121 DASRSC010000000
VERSION     DASRSC010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4789126"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..2929
                     /locus_tag="VFE39_05700"
     CDS             38..2929
                     /locus_tag="VFE39_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HET6258128.1"
                     /translation="MTAGGHVEELGHHSGEGSAGEQVAEANETDGLITDAVAQGDLLR
                     SRQAVFGREVQPQTALYLSRLARAVANAQPQPEGERAAGGATDGGPGAGGPGDDGAEP
                     ATDPCAIQVAELVEPGTDSSGPRVLPALRDAVFGIGPVPPAGPGPEIVPLPSAPALVC
                     DRSQRILRVNAALLKLAGRGTDGSWPFDTARSVNGQGGGLFGMRLPQLVVGPDVDARL
                     VRPDGGLVRVRVVRWELPGSELSAVVLIELDPCPDLDARVVDPRWTAELERLARVGTW
                     TFELATSTLHRSETLEELYRSLGIDPDGAGSAPVEGEQVALLCRGLRSGARDGDHHFD
                     LQLPGNRMLSCWAEVECAKDGTPVRLVGVVHDVSAERVARRRVELSGQRFSDLMAIVP
                     AGVALVDSTGRIVDANTGMCRLLDTSLERLRGAPSTSLSVDELLDPDARPLEATCPGD
                     GAMLPGWLRPVPPGARHAYRVGAVPLRRGDDTTVWCEVSVSVTSADDDGWFWLVACTD
                     ISERRRAAEVLRSAGTVDELTRLPNRAACLELVDRLLAGASRYRVAVVCGDLDDFARV
                     NSSLGHEAGDDLLVTLAARLQRELPVGCTAGRLSGDEFVVICADHTEVGGPDLLARVV
                     ADLLRTTITVHGRPVQLTASVGLATSVPTGDVRAADLLRFAEAAMHDAKRRQSRGGIG
                     MATDGIVSSATQALELEAELRAAIAGDALVLEYQPVVGPDGTVLSAEALVRWPHPDRG
                     LISPADFLPVAQRSGLLRELDLWVLRTAAREAAGWPEYRGRKPAIAVNLAGLLPGDVD
                     FLSAVSDTVAETGLAWDRLVLELVETSLVALPPHALAAMAELVERGLRFAVDDFGTGY
                     SSLARLKELPAQTVKVDRAFVTGIADDPADFAVARAVVDMARAMGRTTVAEGVETADQ
                     FHVLRGIGVDAYQGWLFSRPLPALRLRDVLSRGRLPTPASTPASTPAAT"
     gene            3050..4147
                     /locus_tag="VFE39_05705"
     CDS             3050..4147
                     /locus_tag="VFE39_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6258129.1"
                     /translation="MIKFRGVTKRFPDGTVAVDNLDLALDAGKITVFVGPSGCGKTTS
                     LRMVNRMIEATEGTITIDGRDIMSTDAAELRRGIGYVIQQAGLFPHRTILDNIATVPL
                     LLGWPKARARQRAAELMEIVGLVPEMAQRYPSQLSGGQQQRVGVARALAADPPILLMD
                     EPFSAVDPVVRESLQDELLRLQEDLGKTILFVTHDIDEAVKLGDKVAVFRTGGLLAQY
                     DEPGRLLARPADDFVDAFVGRDRGYRGLGFLSSDALPLGELPTVGLGDPAPERDGWVL
                     VVDDSRQPLGWWHGGARNGATTVVQDHLLPGGSLYEVESGSLRSALDAALSSPSGQGV
                     AVAADGCVAGSVSADDVLRALEQARRHTAAA"
     gene            4161..4817
                     /locus_tag="VFE39_05710"
     CDS             4161..4817
                     /locus_tag="VFE39_05710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6258130.1"
                     /translation="MTWVLGNLEMIWGLTLEHLVFALVPVVLGIVLAVPLGWLANRTG
                     VGRSITINVAGLLYTVPSLALFVILPPLLGTQILDPVNVIVALTVYTVALLVRTVADA
                     LSAVPSMVVNAATAMGYRPLRRFVAVELPLSVPVVVAGVRVAAVSSISLVTVGSVIGF
                     GGLGKMFTDGFQRDIPQEIVAGIVLVLLMALVVDGALVLIGRLLTPWSRVMTTVGMAA
                     "
     gene            4814..5527
                     /locus_tag="VFE39_05715"
     CDS             4814..5527
                     /locus_tag="VFE39_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6258131.1"
                     /translation="MNYLGWLFEPANWSGQGGVPERLLQHVGYTALTMIIALVLAVPL
                     GAWIGHTGRGGFLVVGLANGLRALPTLGLLVLLVGALGIGLIGPLVALVILAVPPILA
                     GTYAGVRNVDNAVVDAARGMGMRGRDVLFRVELPNALPLVVGGIRSSLLQVISTATIA
                     AYVALGGLGRFIVDGLAVRDFPQMIGGSILVAALAIASDLLLAALQKVLVSPGLRVAP
                     TRGRRLRAVRDPRPAAQAA"
     gene            5554..6477
                     /locus_tag="VFE39_05720"
     CDS             5554..6477
                     /locus_tag="VFE39_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6258132.1"
                     /translation="MRRSLIARTAAAAATTVFALTACGGGSDPLQSGGSSAAPSEKII
                     VGSANFTESQILAEIYAQALQAKGVPVETKMSIGSRETYYPGLQDGSIDLIPEYTGVL
                     LQFLNKQATETEPDAVYTALQSALPGKLTVLQKSAAEDKDAVVVTRETATRLHATSIA
                     DLAPNCGQLVFGGPPEFQTRPDGIPGIAKTYGCTFKEYRSLDAGGPLTISALKNGDVQ
                     AADIFTTDASIPADDFVVLADPKNNFAAQNVVPLINKAKVNDTVTQVLNGISAKLTTQ
                     GLTALNAEAASDTKPSAETVAKNWLTQNGLG"
     gene            6474..7316
                     /locus_tag="VFE39_05725"
     CDS             6474..7316
                     /locus_tag="VFE39_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HET6258133.1"
                     /translation="MTVPAGAATAGPAAVGVVSALRRYPIKSMLGEALTEAMLVTGGV
                     EGDRVLALLDASTGRVATAKQPRLWRALLRFTAAHEGAAVRITLPDGRSVGADDEGLD
                     ELLSEVLGRAVVLSDTRPADATVERPAPEDVLAHGVEAEVPYETLEIAQGTTGATFVD
                     YAPVHLITTATLDRIGAEAVRYRPNVVVATPQGVPFAENGWVGREITIGGVRLRGTLP
                     TPRCSVPTLEHGDLPRAAHAVRNLLADNRVDVPGFGVLPCAGVYAEVLDGGVVREGDP
                     VVLN"
BASE COUNT          959 a         2604 c         2684 g         1104 t
ORIGIN      
        1 cgggaatcgg cgtcgttgac ccggtgagat agacgccatg accgctgggg gccacgtgga
       61 agagctcgga caccattctg gtgagggttc ggccggtgag caggttgccg aggcgaacga
      121 gaccgatggg ctgatcaccg acgccgtggc gcagggggat ctgttgcgct cccggcaggc
      181 cgtcttcggc cgcgaggtgc agccccagac ggcgctgtac ctctcccggt tggcccgtgc
      241 ggtggccaac gcgcagcctc agcccgaggg cgaacgggcc gcaggcggcg cgaccgacgg
      301 cgggccgggg gcgggcgggc cgggggacga cggagcggag cccgccaccg acccgtgcgc
      361 catccaggtc gccgagctcg tcgagcccgg taccgactcg agcggaccgc gggtcctgcc
      421 ggcgctgcgg gacgccgtgt tcggcatcgg ccccgtcccg ccggcaggtc cggggccgga
      481 gatcgtcccg ttgccgtcgg cgcctgcgct cgtctgcgac cgctcccagc gcatcctgcg
      541 cgtcaacgcc gcgctgctga agctggccgg ccgtggaacg gacggctcat ggcccttcga
      601 cacagcccgg agcgtcaacg ggcagggcgg cggcctgttc ggcatgaggc tgccccagct
      661 cgtcgtcggc ccggatgtcg acgcccggct tgtgcggccg gacgggggcc tggtgcgcgt
      721 ccgggtggtc cgctgggagc tgccaggctc cgagctgtcg gccgtcgtgc tgatcgagct
      781 ggacccctgc cccgacctcg acgcccgggt cgtggaccca cgctggaccg cggagctcga
      841 gaggttggcg cgggtgggga cgtggacgtt cgagctggcc acgtcgacgc tgcaccgcag
      901 cgagacgctg gaggagctgt accgctcgct cggtatcgac cccgacgggg cgggctcggc
      961 gccggtcgag ggcgagcagg tggcgctgct gtgccgcggc ctgcgctccg gggcacgcga
     1021 cggtgaccac catttcgatc tgcagctgcc cggcaaccgg atgctcagct gctgggccga
     1081 agtggagtgt gccaaggacg gcacgccggt gcggttggtc ggggtcgtgc acgacgtcag
     1141 cgcggagcgg gtggcgcgcc gcagggtcga gctctcggga cagcgcttct cggacctgat
     1201 ggcgatcgtg ccggccgggg tcgcgctcgt ggactccacg ggccgcatcg tcgatgccaa
     1261 cacaggtatg tgccggctgc tggacacgtc gctggagcgg ctgcgtggcg ccccctcgac
     1321 gtcgctgtcg gtcgacgagc tgctcgaccc ggacgcccgg cctctcgagg cgacctgccc
     1381 cggcgacggg gcgatgctcc ccggctggct gcggccggtt ccgccgggcg cccggcacgc
     1441 atatcgggtg ggcgccgtgc ccctgcggcg cggcgacgac accacagtgt ggtgcgaggt
     1501 gtccgtatcg gtcacgtccg ccgacgacga cggctggttc tggctggtcg cgtgcaccga
     1561 catctcggag cgccgcagag ccgccgaggt gctgcgcagc gcgggcaccg tcgacgagct
     1621 gacccggctg cccaaccgcg cggcctgcct cgagctggtg gatcggctgc tcgcgggcgc
     1681 gtcccggtac cgggtggcgg tcgtgtgcgg cgatctcgac gacttcgcca gggtgaactc
     1741 ctcgctcggc cacgaggcgg gtgacgacct gctggtgacg ctggcggcgc ggctgcagcg
     1801 ggagctgccg gtcgggtgca ccgcgggcag gctatccggc gacgagttcg tcgtgatctg
     1861 tgccgaccac accgaggtcg ggggccccga cctcctcgca cgtgtggtcg ccgacctgct
     1921 gcgcaccacg atcaccgtgc acggccgacc ggtgcagctg acggcgtcgg tcgggctggc
     1981 cacctccgtc ccgacgggtg atgtacgcgc cgccgacctg ctccggttcg ccgaggcggc
     2041 gatgcacgac gcgaagcgac gccagagtcg aggagggatc gggatggcca ccgacgggat
     2101 cgtgagctcg gcgacgcagg ccttggagct cgaggccgag ctccgtgcgg cgatcgcggg
     2161 tgacgcgctg gtgctcgagt accagccggt ggtcggcccg gacggcacgg tgctctcggc
     2221 cgaggcgttg gtccgctggc cgcacccgga ccgagggctc atctcgcccg ccgacttcct
     2281 gcccgtcgcc cagcgcagcg gcctgctgcg cgaactggac ctgtgggtgc tgcggaccgc
     2341 ggcgcgggag gccgctggct ggcccgagta ccgcggccgc aagcctgcca tcgccgtcaa
     2401 cctcgccgga ctgctgcccg gcgatgtcga cttcctgtcc gcggtgagcg acacggtggc
     2461 cgagaccggc ctggcctggg accggctggt gctggagctg gtggagacca gcctcgtcgc
     2521 gttaccgcca catgcgctcg ctgcgatggc cgagctggtg gagcgcggcc tgcggttcgc
     2581 cgtcgacgac ttcggcaccg gatactcctc gcttgcccgc ctgaaggagc tgccggcgca
     2641 gaccgtcaag gtcgaccggg cgttcgtcac gggcatcgcg gacgaccccg ccgacttcgc
     2701 ggtggcccgc gcggtggtgg acatggccag ggcgatgggc cgcaccacgg tggccgaagg
     2761 tgtcgagaca gcggaccagt tccacgtgct gcgcggcatc ggcgtggacg cctaccaggg
     2821 atggctgttc tcccgtccgc tgcccgccct gcggctccgt gacgtgctct cccggggcag
     2881 gctcccgacg ccggcttcga cgccggcttc gacgccggcg gcgacgtagg cgcgctccta
     2941 gccgcggtcc cggtccgatc acaactgtcg cgaccgtcgg tcgctcggga tagcctcgaa
     3001 ctacatggcg accggcaagg ggtcgtcggt aaacacggag gtctcccgga tgatcaagtt
     3061 ccggggcgtg accaagcggt tcccagacgg caccgtggct gtcgacaacc tggatctcgc
     3121 gctggacgcc gggaagatca cggtcttcgt cggcccctcc ggctgtggga agaccacctc
     3181 gctgcgcatg gtcaaccgca tgatcgaggc caccgagggc acgatcacga tcgacggacg
     3241 cgacatcatg tccaccgacg cagcggagct gcgtcgtggc atcggctacg tcatccagca
     3301 ggccgggctc ttcccgcacc gcacgatcct cgacaacatc gccaccgtgc cgctgctcct
     3361 cggctggccc aaggcgagag cccggcagcg cgccgctgag ctgatggaga tcgtcgggct
     3421 ggtcccggag atggcgcagc gctacccctc gcagctgtcg ggtgggcagc agcaacgcgt
     3481 cggcgtggcc agggcactcg cggccgaccc gccgatcctg ctcatggacg agccgttcag
     3541 tgcggtcgat ccggtggtgc gggagagcct gcaggacgag ctgctgcgcc tccaggagga
     3601 tctgggcaag accatcctct tcgtcaccca cgacatcgac gaggccgtca agcttgggga
     3661 caaggtggcg gtgttccgca ccggtggcct gctcgcccag tacgacgagc ccgggcggct
     3721 gctggcccgt ccggccgacg acttcgtcga cgccttcgtc ggtcgcgacc gcggctaccg
     3781 cgggctgggt ttcctgtcgt cggacgccct gccgctgggc gagctgccca cggtggggct
     3841 gggcgaccct gcccccgagc gcgacggctg ggtgctggtc gtcgacgaca gccggcagcc
     3901 gctgggctgg tggcacggcg gcgcgcgtaa cggcgcgacc acggtggtgc aggatcacct
     3961 gctgccggga ggttcgctct acgaggtgga gtccggctcg ctgcgcagcg cgctcgacgc
     4021 cgcgctgtcc tcgccgtccg gccaaggtgt cgcggtggcc gccgacgggt gcgtcgccgg
     4081 ctccgtgagc gccgacgacg tgctgcgcgc gctcgagcaa gcccgtcgcc acacggcggc
     4141 ggcgtgagag gggcgctcgc atgacgtggg tgctgggcaa cctcgagatg atctggggcc
     4201 tgaccctcga gcacctggtg ttcgccctcg tcccggtggt gctcggaatc gtcctcgcgg
     4261 ttccgctggg ctggctggcc aaccgcacgg gtgtcggtcg cagcatcacg atcaacgtgg
     4321 ccgggctgct ctacaccgtg ccgtcgctcg cgctgttcgt gatcctcccg cccctgctcg
     4381 gcacccagat cctcgatccg gtcaacgtga tcgtggcgct cacggtgtac accgtcgccc
     4441 tgctggtccg cacggttgcc gacgcgctct ctgcggtccc ctccatggtg gtgaacgcgg
     4501 ccacggcgat ggggtaccgg ccgctgcgtc gcttcgtcgc ggtcgagctg cccctgtcgg
     4561 tccccgtggt ggtcgcgggc gtcagggttg ccgcggtgtc gtcgatcagc ctggtcaccg
     4621 tcggttccgt gatcgggttc ggcggtctgg gcaagatgtt caccgacgga ttccagcgcg
     4681 acatccccca ggagatcgtc gccggcatcg tgctggtcct gctgatggcg ctggtggtgg
     4741 acggcgcgct ggtgctcatc ggtcgcctgc tcaccccgtg gagccgggtc atgaccaccg
     4801 tggggatggc cgcatgaact acctcggatg gctgttcgag ccggccaact ggtcgggcca
     4861 gggcggggtg cccgaacgtc tcctgcagca cgtcggctac acggcgctca cgatgatcat
     4921 cgcgctggtg ctggcggtac cgctcggagc ctggatcggg cacaccggcc gcggcggctt
     4981 cctcgtcgtc gggctggcca acggcctccg tgcgctgccc acgctgggcc tgctggtgct
     5041 gctggtcggc gcgctcggga tcggcctgat cgggccgctg gtcgcgctcg tcatcctggc
     5101 cgtcccgccc atcctcgcgg gcacctacgc gggcgtccgc aatgtcgaca acgcggtggt
     5161 cgacgcggcg cgcggcatgg gtatgcgggg tcgtgacgtg ctgttccggg tcgagctgcc
     5221 caacgcgctt ccgctcgtcg tcggcggcat ccgcagctcg ctgctgcagg tgatctcgac
     5281 ggccacgatc gccgcctacg tcgcgctcgg cggcctcggc cggttcatcg tcgacggtct
     5341 cgccgtacgc gacttcccac agatgatcgg cggctcgata ctggtcgccg cgctcgccat
     5401 cgcctccgac ctgttgctcg ccgcgttgca gaaggtgctc gtctcacccg gcctccgagt
     5461 ggctcccacc cggggccgca ggttgcgggc cgtacgcgat ccccgacccg ccgcgcaggc
     5521 ggcctgatcc ccctcacttc cggaggacca ccgatgagac gctccctgat cgcccgtacg
     5581 gccgccgccg cggccaccac cgtcttcgcc ctcacggcgt gcggcggcgg atccgacccg
     5641 ctccagtccg gcggcagctc cgcagcaccg tccgagaaga tcatcgtggg atctgcgaac
     5701 ttcaccgaga gtcagatcct cgccgagatc tacgcgcagg cgctgcaggc caagggcgtg
     5761 ccggtggaga cgaagatgag catcggcagc cgggagacgt actaccccgg actgcaggac
     5821 ggctcgatcg acctgatccc ggagtacacc ggtgtgctgc tgcagttcct gaacaagcag
     5881 gccaccgaga ccgagccgga cgcggtgtac acggccctgc agtcggcgct gccgggcaag
     5941 ctgacggtgc tccagaagtc ggccgccgag gacaaggacg cggtggtcgt caccagggag
     6001 accgccaccc ggctgcacgc gacgagcatc gccgacctcg ccccgaactg cgggcagctg
     6061 gtcttcggcg ggcccccgga gttccagacc cgccccgacg gcatccccgg catcgccaag
     6121 acctacgggt gcacgttcaa ggagtaccgc tccctcgacg cggggggccc gctgacgatc
     6181 tccgcgctga agaacggcga cgtgcaggcc gccgacatct tcaccaccga cgcgtcgatc
     6241 ccggccgacg acttcgtggt gctcgccgac cccaagaaca acttcgccgc gcagaacgtc
     6301 gtgcctctga tcaacaaggc caaggtgaac gacaccgtca cgcaggtgct caacggcatc
     6361 tcggccaagc tgaccaccca gggtctgacc gccctgaacg ccgaggccgc gagtgacacc
     6421 aagccgagcg cggagaccgt ggcgaagaac tggctcactc agaacggcct ggggtgacgg
     6481 tgccggcggg cgccgcgacc gcaggcccgg ctgccgtcgg ggtcgtctcg gcgctgcgcc
     6541 ggtacccgat caagtcgatg ctcggcgagg cgctcaccga ggccatgctc gtcaccggcg
     6601 gggtcgaagg cgaccgcgtc ctcgccctgc tcgacgcctc caccggccgg gtcgccaccg
     6661 cgaagcaacc ccggctgtgg cgggcgttgc tccggttcac ggcggcccac gagggcgccg
     6721 cggtgcgcat cacgctgccc gacgggcgct cggtcggggc cgacgacgag ggcctcgacg
     6781 agctgctgtc cgaggtgctc ggacgcgccg tcgtgctgtc cgacacgcga cccgccgatg
     6841 ccacggtcga gcggcccgct cccgaggacg tgctggccca cggcgtcgag gccgaggtgc
     6901 cgtacgagac gctggagatc gcgcagggca ccaccggggc gacgttcgtc gactacgccc
     6961 cggtgcacct gatcaccacc gccacgctcg accggatcgg tgccgaggcc gtgcgctacc
     7021 ggcccaatgt cgtcgtcgcg accccgcagg gggtgccctt cgcggagaac ggatgggtcg
     7081 gccgggagat cacgatcggc ggggtgcggc tgcgcggcac gctgcccacc ccgcgctgct
     7141 cggtgccgac ccttgagcac ggtgacctgc cgcgggctgc gcacgccgtg cgcaatctgc
     7201 tggcggacaa ccgggtcgat gtccccggct tcggcgtgct gccgtgtgcc ggtgtctacg
     7261 ccgaggtgct cgacggcggg gtcgtgcggg agggcgaccc tgtcgtcctg aactgaacgg
     7321 gaggcgtcag cgatagccgg tggcgtccgc g
//
LOCUS       DASRSC010000122         3134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3968401,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000122 DASRSC010000000
VERSION     DASRSC010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3968401"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VFE39_05730"
     CDS             complement(<1..1074)
                     /locus_tag="VFE39_05730"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HET6258134.1"
                     /translation="MPTATDLPGVAELLEAAVTAVGGRRREGQDAMAQAVRKAITSGE
                     HVAVQAGTGTGKSLAYLVPAIHHAVAKDATVVISTATIALQRQLVDRDLPRLAKTLKP
                     LLGRAPTFAILKGRRNYLCLNKLHGGDDTDPGDELFDPFAISAMGRQIKRLHEWADTT
                     EKGDRDELVPGVPDSTWRQVSVTARECLGAARCPVGDDCFAEKARAEAGKSDIVVTNH
                     ALLAIDALEGRPVLPEHDVVVVDEAHELVDRVTGVATAELTAGMIGAAARRLGKLVDQ
                     AIADRLAEAGEGLAMVLEDLPPGRWDTLPQAAAGALSAVRDAAGSCRQLLGSERREDP
                     EAATGRKIAQASLDEVADTAVRLL"
     gene            1212..2117
                     /locus_tag="VFE39_05735"
     CDS             1212..2117
                     /locus_tag="VFE39_05735"
                     /inference="COORDINATES: protein motif:HMM:NF044313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258135.1"
                     /translation="MTSTQLLLRPSSYPVQIEHTAYYSDLDPSLRIGRDAIVRWFEDA
                     RIAVERDVFGAGLTARMRLLLASVRVDVLAPLQVVARSAAGARIDTGHYRIGIGVSHI
                     GTSSFAYTYGVFAGEECVATGESVSVHATVGGPAPLPEPVRAALETLHMDAPAPTRLE
                     RWPSRLVREAYPFRLDVRTRFGDLDPNRHVNNVALAGWYLDGLAELHLDVLGYPTGGP
                     LDGLSPSSLRVDYLDEVHYPGIYQLRVGVLELDETSVRYACGLFDGPRCIGLAEAVGA
                     HRIPDEDGDPGDLAARLEPFRMRAV"
     gene            complement(2125..2691)
                     /locus_tag="VFE39_05740"
     CDS             complement(2125..2691)
                     /locus_tag="VFE39_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HET6258136.1"
                     /translation="MTRALIVVDVQNDFCEGGSLGVDGGAAVAAAISIRLQAAGQEAR
                     WDHVVASRDHHGDPGGHFSDAPDFVDSWPPHCRRGTPGASFHPELDVARIEAVFDKGH
                     DTAAYSAFEGTEPGGAGLRDWLRAHDVDALDVVGIATDHCVRATALDAAAAGFSTTVL
                     LDLCVGVAPETTQRAIAQMRAAGVAVLG"
     gene            complement(2695..>3134)
                     /locus_tag="VFE39_05745"
     CDS             complement(2695..>3134)
                     /locus_tag="VFE39_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET6258137.1"
                     /translation="LDEYAIAALRAEPVDSYGVGTSVVTGSGAPTAGMVYKLVEVDGR
                     PVAKRSAAKESRGGRKSAIRRHKPTGTAIEEVVFPVTAPPEAGPHDRVVQIPLMRGGD
                     RVADLPTLEQSREYLRAALVSVPWEGLKLSRGEPALPTVFDGV"
BASE COUNT          398 a         1164 c         1133 g          439 t
ORIGIN      
        1 gagcagccgg acggcggtgt cggccacctc gtccagcgag gcctgggcga tcttgcggcc
       61 ggtggcggcc tccggatcct cccgacgctc cgacccgagc aactgacggc acgagcccgc
      121 ggcgtcacgc accgcagaca gggcgcccgc ggctgcctgc gggagcgtgt cccagcgccc
      181 gggcggcagg tcctccagca ccatcgcgag gccctcgccc gcctcggcga gccggtcggc
      241 gatcgcctgg tcgacgagct tgcccaggcg gcgggcggcc gcgccgatca tgccggcggt
      301 cagctcggcc gtggccactc cggtgacccg gtcgaccagc tcgtgcgcct cgtcgaccac
      361 cacgacgtcg tgctcgggca gcaccgggcg accctccagc gcgtcgatcg cgagcagcgc
      421 gtggttggtg acgacgatgt cggatttgcc ggcctcggcg cgggccttct cggcgaagca
      481 gtcgtccccg acggggcacc tcgcggcccc caggcactcg cgggcggtga ccgacacctg
      541 gcgccacgtg gagtcgggca cgccgggcac cagttcgtcg cggtcgccct tttcggtggt
      601 gtcggcccac tcgtgcagcc gcttgatctg gcggcccatc gcggagatcg cgaaggggtc
      661 gaacagctcg tcgcctgggt cggtgtcgtc gccaccatgc agtttgttca ggcacaagta
      721 gttgcgacgg cccttgagga tcgcgaacgt gggcgcgcgc ccgagcagcg gcttgagcgt
      781 cttggccagg cgcgggaggt cgcggtcgac gagctggcgc tgcagcgcga tcgtggcggt
      841 ggagatgacg acggtggcgt ccttcgccac cgcgtggtgg atggccggca cgaggtaggc
      901 cagcgacttg cccgtgccgg tgccggcctg cacggccacg tgctccccgc tggtgatggc
      961 cttgcggaca gcctgggcca tcgcatcctg gccctcgcgg cgccggccgc cgaccgcggt
     1021 gaccgccgcc tccagcagct ccgccacgcc gggcagatcg gtcgcggtgg gcaccccggg
     1081 agggtacctg ccctgcccgg ccggccgagg tcgaaccacc gatccaggat ccacgggcgt
     1141 tgcgatggtc acacgtgacc cacagcgggg ggtctccccc tgcccgggcg ggtgccgaca
     1201 tcatgagcag agtgacgtcc acgcaactcc tgctccgccc ctcgtcctac cccgttcaga
     1261 tcgagcacac ggcctactac agcgacttgg acccgagcct gcgcatcggg cgcgacgcga
     1321 tcgtccgctg gttcgaggat gcgcgcatcg ccgtcgagcg cgacgtgttc ggtgcgggtc
     1381 tcaccgcgcg gatgcggctg ctgctcgcct cggtccgggt cgacgtgctg gccccgctgc
     1441 aggtcgtcgc ccgcagcgct gccggggcgc gcatcgacac gggtcactac cgcatcggta
     1501 tcggggtcag ccacatcggc acctcgtcgt tcgcctacac ctacggcgtc ttcgcgggcg
     1561 aggagtgcgt cgccaccggc gagtcggtca gcgtgcacgc gaccgtgggg ggaccggcgc
     1621 cgctgcccga gccggtgcgc gccgcgctgg agaccctgca catggacgct cccgcgccca
     1681 cccgcctgga gcgctggccg tcccggctgg tccgggaggc gtacccgttc cggctggacg
     1741 tccgcacccg cttcggcgac ctcgacccca accggcacgt gaacaacgtg gcgctcgccg
     1801 gctggtacct cgacggcctc gccgagctgc acctcgacgt cctggggtac ccgaccggcg
     1861 gcccgctcga cgggctctcg ccgagctcgc tgcgcgtgga ctacctggac gaggtccact
     1921 acccgggcat ctaccagctc cgggtcgggg tgctcgagct ggacgagacc tcggtgcgct
     1981 acgcgtgcgg actgttcgac gggccacgct gcatcggact cgccgaggcg gtcggcgccc
     2041 accgcatccc ggacgaggac ggcgaccccg gcgacctcgc cgcgcgcctg gagcccttca
     2101 ggatgcgcgc ggtctgacag cccgctaccc gaggacggcg acgcccgcgg cccgcatctg
     2161 cgcgatcgcg cgctgagtgg tctccggggc cacgcccaca cacaggtcga ggagcactgt
     2221 cgtcgagaac ccggccgccg ccgcgtcgag cgcggtggcc cggacgcagt ggtcggtggc
     2281 gatgcccacc acgtccagcg cgtcgacgtc gtgggcacgc agccagtcgc gcagccccgc
     2341 gccgcccggc tcggtgccct cgaaggccga gtacgcggcg gtgtcgtggc ccttgtcgaa
     2401 caccgcctcg atccgggcga cgtcgagctc ggggtggaag gacgcgccgg gcgtgccgcg
     2461 acggcagtgc ggcggccacg agtcgacgaa gtcgggggcg tcggagaagt ggccgccggg
     2521 gtcgccgtgg tggtcgcggc tggcgacgac gtggtcccag cgggcttcct ggcctgcggc
     2581 ctgcagccgg atcgaaattg cggccgccac cgcggctccg ccgtccacgc cgagcgaacc
     2641 gccctcgcag aagtcgttct gcacgtccac gacgatcagg gcgcgcgtca tcggtcagac
     2701 tccatcgaaa accgtaggga gtgcgggctc gccgcgggag agcttgagcc cctcccacgg
     2761 cacggacacg agcgcggccc gcaggtactc gcgggactgc tcgagtgtcg gcaggtccgc
     2821 cacccgatca ccgccgcgca tcagcggtat ctgcaccacc cggtcgtgcg gcccggcctc
     2881 cggcggtgcg gtgacgggga acacgacctc ctcgatggcg gtgcccgtcg gcttgtggcg
     2941 ccggatcgcc gacttccgtc cgccgcggga ctccttcgcc gcgctgcgct tggccacggg
     3001 cctgccgtcg acctccacca gcttgtagac catgccggcc gtcggtgcgc cggaaccggt
     3061 gaccaccgac gtgccgacgc cgtaggagtc gacgggttcg gcgcgcagtg cggcgatcgc
     3121 gtactcgtcg aggt
//
LOCUS       DASRSC010000123         3717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4297543,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000123 DASRSC010000000
VERSION     DASRSC010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3717
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4297543"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..806)
                     /locus_tag="VFE39_05750"
     CDS             complement(18..806)
                     /locus_tag="VFE39_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6258138.1"
                     /translation="MRRTVLTSDGLDLAVVEFGGAGPPILLLHGLMGRATTWWSVAQW
                     LTRYGRVLGLDARGHGRSDARGPWTTGRMTADAAELLADVGPGVVIGHSMGGLHGLVL
                     AARHPELVRALVVEDMGVDFRGRSVEDSRAWFGALNRPFASLAAVRQTFGRPRLEFGD
                     YMAECVEERTDGYHLLARVEHAIGIATEWAERDHWDAVASVRCPVLLIEAEESVAPPG
                     QMAQMARGMPLARHVLVPGTGHLVHAGDPAAYRSVVEKFMSTHM"
     gene            826..1131
                     /locus_tag="VFE39_05755"
     CDS             826..1131
                     /locus_tag="VFE39_05755"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET6258139.1"
                     /translation="MAVVKINAIEVPEGAGPELEKRFANRLHAVDGQPGFISFELLRP
                     VSGETRYFVLTHWEDEESFQAWRNGPAAEAHGGERAKPVASGASLLEFEVVQRSTAS"
     gene            1203..1955
                     /locus_tag="VFE39_05760"
     CDS             1203..1955
                     /locus_tag="VFE39_05760"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HET6258140.1"
                     /translation="MGVDSGLPDFRGPEGFWRAYPPYRALGLRFEEIADPVHFAADPE
                     LAWGFYGHRLALYRSTVPHVGFGVLLKWAQRIPVRVFTSNVDGQFQRAGFPDEHVTEC
                     HGSIHFLQCTVDCGQPVWSADAVSVEVDPVTMRAAGPLPSCPECGAPARPNILMFGDL
                     DWIADRSADQMRAHNTWLRELRASGRRLAVVELGAGTALPTVRREAERASAASGALVR
                     INPREPDVRHGRGVSIAAPAAATLLALHELLG"
     gene            complement(1962..2855)
                     /locus_tag="VFE39_05765"
     CDS             complement(1962..2855)
                     /locus_tag="VFE39_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6258141.1"
                     /translation="MMMTSVTCHLHRPEGRRVAVHDLTPGAPADAPVVLMCHAAPGSG
                     SFDPDPAETAGRGIRLIAPDRPGYGGSDPMGDGAFATVETAADDAAAVLESFLAPGAT
                     AAVAGWSAGGRVGLALAARRPELVSRVAVIGTPAPDEDVPWIPDEKRAGVEALRGAPA
                     AVAHEALGAAFGPMLAALAGDARFGLVLIDEVDGPVLAEPGVADRVRAMLDAALAQGG
                     AGMVADIVGYTLRPWGFDPADVTASVLLGYGSADAIGHAHGAWWQRALPDARLEMVPA
                     TGHLVVVPFWNQVLAHLVPRG"
     gene            2920..3615
                     /locus_tag="VFE39_05770"
     CDS             2920..3615
                     /locus_tag="VFE39_05770"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET6258142.1"
                     /translation="MNRTERLYAIAEELRASGPAGRTGAWLAQRLEVSTRTIKRDITA
                     LLQTGLPLWAQAGPGGGYVLDAATLPPLNITGAEAAAIAVALAALPALPFAVDGRSAL
                     SKVLAAMPAPERERAEAIAGRLWLRAPEQTPRPAVARVLDEAVRRCQVLVLHYTGRAG
                     DVTERAVEPVALAATNGHWYLLAWCRLRQGPRWFRTDRITSAHLSAERAPEHDAATLF
                     GTPPDDARPVTLR"
BASE COUNT          526 a         1383 c         1300 g          508 t
ORIGIN      
        1 gtcgatgtag agctcgctca catatgagta ctcatgaact tctcgacgac cgagcggtag
       61 gcagctggat cgcccgcgtg caccaggtgg ccggtgcccg gaaccaggac gtggcgagcc
      121 agcggcatgc cgcgcgccat ctgcgccatc tgaccgggcg gcgcgacgct ctcctcggcc
      181 tcgatcagca gcaccgggca gcggaccgac gccacagcgt cccagtggtc acgctcggcc
      241 cactcggtcg cgatgccgat tgcgtgctcg acacgggcca gcaggtggta accgtcggtc
      301 cgttcctcga cgcactcggc catgtagtcg ccgaactcga ggcgtggccg gccgaacgtc
      361 tgccgcaccg ccgcgaggga ggcgaacggc cgattgagcg cgccgaacca ggcacgggag
      421 tcctccacgc tgcgaccccg gaagtccacg cccatgtctt cgacgaccag cgcccgcacc
      481 agctccggat gccgcgcggc gaggaccagc ccgtgcagcc cgcccatcga gtgtccgatc
      541 accaccccgg gtccgacgtc ggcgagcagc tccgccgcgt cggccgtcat gcgcccggtg
      601 gtccacgggc cccgcgcgtc ggaccggccg tggccgcgag catccaggcc gagcacccgg
      661 ccgtaccggg tcaaccactg cgcgacggac caccacgtcg tcgcccggcc catcagcccg
      721 tgcagcagca ggatcggcgg ccccgcaccg ccgaactcga ccaccgcgag atcgaggccg
      781 tcgcttgtca gcacggtccg ccgcatcgcg gttagcctag gtgtcatggc cgtcgtgaag
      841 atcaatgcga tcgaggtacc cgagggcgct gggcccgagc tggagaagcg gttcgccaat
      901 cggctgcacg ccgtggacgg gcaacccggc ttcatctcct tcgagctgct gcgcccggtc
      961 agcggggaga cccgctactt cgtgctgacg cactgggagg acgaggagtc gttccaggca
     1021 tggcgcaacg gcccggcggc ggaagcgcac ggcggcgagc gcgccaagcc ggttgcgtca
     1081 ggggcgtcgt tgctcgagtt cgaggttgtg cagcgctcca cggcgtcctg aactccgcgc
     1141 ttgcgtccgc cgtcgcgcag atctccggcg cggacgcgct actggtgtgt gccggcgcgg
     1201 gaatgggcgt cgactccggc ttgcccgact tccgcgggcc ggagggcttc tggcgggcgt
     1261 acccgccata ccgtgcgctg ggtctgcggt tcgaggagat cgccgacccc gtgcacttcg
     1321 ccgccgatcc agagctggcc tggggtttct acgggcaccg actggcgctg taccggagca
     1381 cggtgccgca tgtcggtttc ggcgtcctgc tgaagtgggc gcagcgaata cccgtgcggg
     1441 tcttcacatc gaacgtggac ggccagttcc agcgcgccgg cttcccggac gagcacgtga
     1501 ccgagtgcca tggctcgatc cacttcctgc agtgcacggt ggactgcggg cagccggtgt
     1561 ggtccgccga cgccgtctcg gtagaggtcg atccagtcac gatgcgcgcc gccgggccat
     1621 tgccgtcgtg cccggagtgc ggcgccccgg cccggcccaa catcctgatg ttcggcgacc
     1681 ttgactggat cgcggaccgc tccgccgacc agatgcgggc gcacaacacc tggctgcgcg
     1741 aactgcgggc gtcgggtcgc cggctcgcgg tcgtcgagct gggagcgggt actgcgctcc
     1801 cgacggtgcg ccgcgaggcc gagcgagcca gcgccgcgtc cggggcgctg gtccggatca
     1861 atccccgtga gccggacgtg cggcacgggc gcggagtgag cattgccgcc cccgccgccg
     1921 ccaccctgct cgccctgcac gagctcctcg ggtgacgctt ctcaccctcg gggcacaaga
     1981 tgcgcgagaa cctgattcca gaacggcacg accaccaggt gcccggtagc cgggaccatc
     2041 tccagccggg cgtcgggcag cgcccgctgc caccacgcgc cgtgcgcgtg gccgatggcg
     2101 tccgctgagc cgtagccgag cagcacgctc gccgtgacgt ctgccgggtc gaaaccccac
     2161 ggccgcagcg tgtagccgac gatgtccgcc accatcccgg ccccaccctg ggcgagcgcc
     2221 gcgtcgagca tcgcgcgcac ccgatcggcg acgccgggtt ccgcgagcac tggtccgtcc
     2281 acctcgtcga tcaggacgag cccaaacctg gcgtcgccgg cgagcgccgc cagcatcggc
     2341 ccgaacgcgg ctcctagcgc ctcgtgcgcg acggcggccg gcgcacctcg gagcgcctcg
     2401 acacccgcgc gtttctcgtc cgggatccac ggcacgtcct cgtccggcgc cggggtgccg
     2461 atcacggcca cccgagagac cagctcggga cgccgggccg cgagcgccag gcccacccgg
     2521 ccgccggccg accagcccgc aaccgcagcc gtggcgccag gcgccagaaa gctctccagt
     2581 accgccgccg catcgtcagc cgccgtctcc accgttgcga acgccccgtc gcccatgggg
     2641 tcggaaccgc cgtaaccggg ccggtcgggt gcgatcagcc ggatcccgcg cccggccgtc
     2701 tccgcagggt cggggtcgaa gctgccggag cccggggccg catggcacat cagcaccacc
     2761 ggcgcatcgg caggcgcgcc gggcgtcagg tcgtgcaccg cgacgcggcg cccttcgggg
     2821 cggtggaggt ggcaggtgac gctcgtcatc atcatgggac aagcttcgca acaggtcggt
     2881 gacaccgtgc tgtcaccgtt ttccgggcat tctcgacacg tgaaccgcac cgaacggctg
     2941 tacgcgatcg ctgaggagct tcgcgcctcg ggcccggccg ggcgaaccgg cgcctggctg
     3001 gcccagcgac tcgaggtctc gacgcggacg atcaagcgcg atatcacggc cctgctccag
     3061 accgggctcc cgctctgggc acaggccggt cccggcggtg gttacgtcct ggacgccgcc
     3121 acgctgccgc cgctgaacat caccggtgcc gaagcggccg caatcgcggt ggcgctggcc
     3181 gcattgccgg ccctgccgtt cgcggtcgac gggcgcagcg cgctgtcgaa ggtcctcgcc
     3241 gccatgccgg cgccagagcg ggaacgcgcc gaggcgatcg ccgggcggct gtggctacgg
     3301 gcgcccgagc agacgccccg ccctgccgtc gccagggtgc tggatgaagc tgtgcggcgg
     3361 tgtcaggtgc tcgtgctgca ctacacgggc cgggcggggg acgtcacaga acgtgccgtt
     3421 gaaccggtgg ccctggcggc gacgaacggg cactggtacc tcctcgcgtg gtgcaggctg
     3481 cgccagggac cgcggtggtt ccgcaccgac cggatcacgt ccgcgcatct ctccgcggaa
     3541 cgcgcgcccg agcacgacgc tgccaccctg ttcggaacgc cgcccgacga cgcccgcccc
     3601 gtgacgctcc gctgagtcgg cggtatcaac tcgccccaga tcgagttact cgctcgcccc
     3661 agatcgggtc actcgccggg gggccgggtc agcaaggtgg ccagcgtgct cgtgcag
//
LOCUS       DASRSC010000124         7888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2333126,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000124 DASRSC010000000
VERSION     DASRSC010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2333126"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /gene="pcaH"
                     /locus_tag="VFE39_05775"
     CDS             complement(<1..661)
                     /gene="pcaH"
                     /locus_tag="VFE39_05775"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HET6258143.1"
                     /translation="MSVTIPHPTGYRAAPEGNHPPLLSEGYKSTVLRAPSQAPVVLRH
                     RLTEVTGPLLTGDIGVNDHDLTAQHDGEPVGQRIVVLGRVLDSDGRPVPDTLVEVWQA
                     NAAGRYAHARDTWPAPLDPNFSGGGRCLTDSEGRFEFITIKPGAYPWGNHHNAWRPAH
                     IHFSLFGRAFTQRLVTQMYFPDDPFFPYDPIYNSVRDETARQRMISTFNIHETRPNWA
                     QA"
     gene            complement(728..1897)
                     /locus_tag="VFE39_05780"
     CDS             complement(728..1897)
                     /locus_tag="VFE39_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HET6258144.1"
                     /translation="MRTQVGIVGAGPAGLLLSHLLARSGVDSVVLEARSRAYVERRVR
                     AGVLEHPTVELLREVGLAHRLDREGMRHEGISLRFDGGDHRIDFADLVGRGITVYGQQ
                     EVVKDLIAARLDDGGDIRFEVSDVTLDGLADSPVIGFTGADGRREELHCDVVAGCDGF
                     HGVSRGAFVPEVFDRQYPFAWLGILAKAAPTHHELVYANHSNGFALYSMRGPEITRLY
                     LQVPPDTDAGAWSDAQIWDELDRRLAAEGFTLNEGPVIEKGVTGMRSFVAEPMRHGRL
                     FLAGDAAHIVPPTGAKGMNLAIADVHVLAEALVPFLRHGDETGVDAYSDTCLRRVWRA
                     EHFSWWMTSMLHVAPDDDPIARRLQLSQLRYTVASRAAATSLAENYVGLPHGESH"
     gene            1977..2738
                     /locus_tag="VFE39_05785"
     CDS             1977..2738
                     /locus_tag="VFE39_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019813980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET6258145.1"
                     /translation="MSQSVTARVLRVIAAFSAERPELTLTELSRRAALPLSTVHRLVG
                     ELAEWGALERAADGRYRVGLRLWEVGALAPRGLGLRESAMPFLEDLYAVTKQNVQLAV
                     LDGSEVVYLERLSGRGAVNVITRVGGRLPLHATGVGLVLLAFAAPELQEQILAAPLKR
                     YTAKTMCTPDELRRALAEVRRTGVAVSDGQIELIALSVAAPVRGPRGGVVAALSVVVP
                     SETSDAMAYVPVVRAAARGISRTLGFPDSPAAERK"
     gene            2877..4067
                     /locus_tag="VFE39_05790"
     CDS             2877..4067
                     /locus_tag="VFE39_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015102884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6258146.1"
                     /translation="MSATLLVSDLDPFAPEFLGDPYPAHAALRDAGPVVRLERYRTWA
                     SARHAPVRAALLDHETFCSSAGVGISDFRKEEPWRTPSLLLEADPPEHTRARRVVAGV
                     LSAAAMRRLAEDFMSAADELVARLADGEPFDAMTDLATAFPVHVFSEAVGVPDDKREN
                     LLTYARMVFNTFGPRNELFRNAVADAGPVRDWIAQRCRAENLLSGGMGAEIHARAAAE
                     GFTGEESAMLVRSFLSAGVDTTVHGLGNALWCLAQHPEQFAALRSEPDRARAAFEEVL
                     RFEAPVQTFFRTTTREVELDGVTIGDGEKILLFLGAANRDPRQWPDADVFNISRRATG
                     HVGFGFGIHACVGMAMARLEGEAVLTALARHVRKIEPTGEPRRMLNNTLRGLESLPLR
                     LVPA"
     gene            4131..5129
                     /locus_tag="VFE39_05795"
     CDS             4131..5129
                     /locus_tag="VFE39_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET6258147.1"
                     /translation="MDVVEFRPEPEQYAWTFGGVAPTHRITPGTVLKLWTEDAFSGRL
                     QRTSDKPSQVLDMTQVNPQTGPFHVEGAEPGDTLALHIVDLTPARDWAASTTIPFFGA
                     LTGTDRTALLNDPLPEQTWIYQLDRDRGTVLFEAQRSKLSVELPIAPMLGTVGVAPPG
                     REVRSSLVPDTFGGNMDTPEMRAGTTCFLGVNVEGALFSVGDGHYRQGEGESCGTAVE
                     GAMDVTLIVELVKGGAPAWPRLEHDDHYAVVGSSRPLEDAWRASQVGMIGWLGDLYGL
                     DPLDAYQLLTQISRSPLANVCDTNYSAVTKIEKALLPAADAYGGMHRHLREQARIL"
     gene            5144..5902
                     /locus_tag="VFE39_05800"
     CDS             5144..5902
                     /locus_tag="VFE39_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6258148.1"
                     /translation="MDLQLTGRTALVTGGTKGIGRAVVEGLAAEGARVAFCARTDADV
                     AKVEQELQAAGHDVAGTAVDVADAEALTAWVAASAERFGGIDVVVANVSALSIGPGPE
                     HWQPSFEVDLMHTVRLVDAALPHLEASGVGSVIAVSSVSAREIDFASGAYGVMKAALV
                     HYTSGLAVQHAGKVRANSVSPGNTYFEGGVWQSLENSDPEFFANTLRVNPTGRLATPE
                     EIAYGIIMLASPRASFITGAHLVVDGALTRGVQL"
     gene            5904..7037
                     /locus_tag="VFE39_05805"
     CDS             5904..7037
                     /locus_tag="VFE39_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HET6258149.1"
                     /translation="MREFRVQIPEADLQDLRGRLARTRWPEPATAPGWTQGVPLDYAR
                     ELCDYWAREYDWRRCEAELNALPQFRTGLDGGGDDTVEVHLIHARSRHPGALPLLLSH
                     GWPGSVVEFLGVIEALTDPPDPRDAFHVVAPSLPGYGFSGKPTVPGWGVERIATAWAQ
                     LMDRLGYDRYGAQGGDWGSMITSALGTGAPQNLVGIHLTMPLAAPPPENDGVRLTESE
                     HAALAARKTFQKVGTGYSSEQSTRPQTLGYGLLDSPSGQCMWIVEKFWDWTDCAGHPE
                     NVITRDRLLDNVMLYWLPGTGASSARLYWESYKRRRMDEVTVPTGVTLFPQELVRLPR
                     QWIERRFTDLRHWSEPATGGHFASLEQPDVFVDELRTFFRPLR"
     gene            complement(7194..>7888)
                     /locus_tag="VFE39_05810"
     CDS             complement(7194..>7888)
                     /locus_tag="VFE39_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HET6258150.1"
                     /translation="FGLPESEIAATLRDLGAGRDLSGVEVTTCLRRSELEVDLRPRPD
                     AADVAAELHAGLAARHAKHLVSADGTSTDELLAAALLERGWTVATGESCTGGMLASRL
                     IDRAGSSAYVAGGVVAYSNEAKSQLLGVPAEMIDEHGAVSPEVARALADGARERFGAD
                     IGIGITGVAGPGGGTEAKPVGYVCFCVTTGDGVELARDPRLPGGRSDVRERSTDLGMH
                     LLLRTVAPRPPV"
BASE COUNT         1142 a         2820 c         2819 g         1107 t
ORIGIN      
        1 acgcctgcgc ccagttcggc cgggtctcgt ggatgttgaa ggtcgagatc atccgctgac
       61 gtgcggtctc gtcccgcacc gagttgtaga tcgggtcata cgggaagaac ggatcgtcgg
      121 ggaagtacat ctgcgtcacc agccgctgcg tgaacgcccg gccgaacagc gagaaatgga
      181 tgtgcgcggg gcgccaggcg ttgtggtggt tgccccacgg gtaggcaccg ggcttgatcg
      241 tgatgaactc gaaacggcct tcggagtccg tcaggcagcg cccgccgccg ctgaagttcg
      301 ggtcgagcgg ggcgggccag gtgtcgcggg cgtgggcgta gcggcccgcg gcgttggcct
      361 gccacacctc gacgagcgtg tcgggaaccg ggcggccgtc gctgtcgagc acccggccga
      421 gaacgacgat ccgctgcccg accggctcgc cgtcgtgctg cgcggtgagg tcgtggtcgt
      481 tgacaccgat gtcaccggtg agcagcgggc cggtcacctc ggtgagccgg tggcgcagca
      541 ccaccggcgc ctgggacggc gctcgcagga cggtgctctt gtacccctcg gacaggaggg
      601 gtgggtggtt cccctccggg gccgcgcggt agccggtggg atgcggaatt gtcacggaca
      661 gctcctcgct agcgctcgtc gccaccgtga cgacgacgct gtgttgatgg tgacctcgca
      721 agctcggtca atgactctct ccatggggca ggccgacgta gttctcggcg aggctggtgg
      781 ccgcagcgcg ggaggccacg gtgtagcgca gctgggacag ctgtagccgg cgggcgatcg
      841 ggtcgtcgtc cggggcgacg tgcagcatcg aggtcatcca ccacgagaaa tgctccgcgc
      901 gccagacccg ccgcaggcac gtgtcggagt acgcgtcgac cccggtctcg tcgccgtggc
      961 gcaggaacgg gacgagcgcc tcggccagca cgtgcacgtc ggcgatcgcg aggttcatgc
     1021 ccttcgcgcc ggtcggcggg acgatgtgcg cggcgtcgcc cgccaggaac agccgcccgt
     1081 gccgcatcgg ttcggcgacg aagctacgca tgccggtgac gcccttctcg atcaccgggc
     1141 cctcgttgag cgtgaacccc tcggccgcga ggcggcggtc cagctcgtcc cagatctgcg
     1201 cgtcggacca tgcgccggcg tcggtgtccg gcgggacctg caggtagagc cgggtgatct
     1261 cggggccgcg catcgagtac agcgcgaacc cgttggagtg gttggcgtac acgagctcgt
     1321 ggtgcgtggg cgcggccttg gcgaggatgc cgagccaggc gaacgggtac tgccggtcga
     1381 acacctccgg gacgaacgca ccgcggctga ccccgtggaa cccgtcgcac ccggcgacga
     1441 cgtcgcagtg cagctcctcg cgccgtccgt cggcgccggt gaacccgatc accggggagt
     1501 cggcgagccc gtcgagcgtc acgtcggaca cctcgaaccg gatgtcccca ccgtcgtcca
     1561 ggcgcgcggc gatgaggtcc ttcaccacct cctgctggcc gtagacggtg atcccccgcc
     1621 cgaccagatc ggcgaagtcg atgcggtggt cgccaccatc gaagcgcagc gagatgccct
     1681 cgtgccgcat gccctcgcgg tccagccggt gggcgagccc gacctcgcgc agcagctcca
     1741 cggtcgggtg ttccagcaca ccggccctga cccgccgctc cacgtaggca cggctgcggg
     1801 cctcgaggac cacggagtcg accccggagc gggccagcag gtgggacagc agcagtccgg
     1861 ccggtcccgc cccgacgatc ccgacctgag ttcgcatgcg aggagcctgg cccacggggc
     1921 cacacgcgct cgactcggct tccggtgaac ggaagaccag ggcacactgg gacgtgatga
     1981 gtcagtcggt cactgcacgg gtcctccggg tgatcgcggc gttcagtgcg gagcgtccgg
     2041 agctcacgct cacggagctc agcaggcggg ccgccctccc gctgagcacc gtgcaccggc
     2101 tcgtcggcga gcttgcggag tggggcgcgc tcgagcgcgc cgccgacggt cgctaccgcg
     2161 tcgggctccg gctgtgggag gtcggtgccc tggccccgcg cgggctgggc ctgcgcgagt
     2221 cggcgatgcc gttcctcgag gacctctatg cggtgaccaa gcagaacgtg cagctcgcgg
     2281 tgctcgacgg tagcgaggtg gtgtacctgg aacgactgtc gggacggggc gcggtcaacg
     2341 tcatcacgcg cgtcgggggc cggctgccac tgcacgcaac gggcgtcggg ctcgtcctgc
     2401 tcgccttcgc cgcacccgag ctacaggaac agatactcgc cgccccgctc aagcgctaca
     2461 ccgccaagac gatgtgcaca cccgacgagc tgcgcagggc gctcgccgag gtgcgccgca
     2521 ccggggtggc ggtgagcgac gggcagatcg agctgatcgc gctgtccgtc gcggcgccgg
     2581 tgcgcgggcc acgcggcggc gtggtcgcgg cgctgtcggt cgtcgtgccg agcgagacct
     2641 ccgacgccat ggcctacgtg cccgtcgtcc gcgccgcagc gcggggcatc tcccggacgc
     2701 tcggcttccc cgattcccct gccgcggaac ggaaatgacg cggcggaaag gccggccctc
     2761 cagccgatcg tgacgaccac cacacggaag gggagtgttc gcatgagcga gcttgcgagc
     2821 gaatcaatgg cacagcgccc gagcgaagcg ccggccgagc gaagcgaggg cgcgcggtga
     2881 gcgccacgct gctggtgagc gacctcgacc cgttcgcgcc cgagttcctc ggcgacccgt
     2941 acccggcaca tgccgcactg cgtgacgccg ggccggtcgt ccggctggag cggtaccgca
     3001 cctgggccag cgcgcggcac gccccggtgc gtgcggcact tctcgaccac gagacgttct
     3061 gctcgtccgc gggcgtcggc atctcggact tccgcaagga ggagccgtgg cgcacgccgt
     3121 cgctgctgct ggaggccgac ccgcccgagc acacccgcgc ccgccgggtg gtggccggcg
     3181 tgctgtcggc agcggcgatg cggcgcctgg ccgaggactt catgtccgcg gctgacgagc
     3241 tcgtcgcgcg cctggcggac ggagagccct tcgacgcgat gacggacctc gcgaccgcct
     3301 tcccggtgca cgtgttctcc gaggccgtcg gcgtgccgga cgacaagcgg gagaacctgc
     3361 tcacctacgc gcgcatggtg ttcaacacgt tcgggccgcg caacgagctg ttccgcaacg
     3421 ctgtggccga cgcgggcccg gtccgcgact ggatcgcgca gcgctgccgg gcggagaacc
     3481 tcctgtccgg cggaatgggc gccgagatcc acgcgcgggc ggcggccgag gggttcacgg
     3541 gggaggagag cgccatgctc gtgcggtcgt tcctctccgc cggggtcgac accaccgtgc
     3601 acggcctcgg gaacgcgctc tggtgcctgg cccagcaccc cgagcagttc gccgcgctgc
     3661 gttcggaacc cgaccgggcg cgcgccgcct tcgaggaggt gctccgcttc gaggcgcccg
     3721 tgcagacgtt cttccgcacc accacccgag aggtggagct cgacggcgtc acgatcggcg
     3781 acggcgagaa gatcctgttg ttcctcggcg ccgccaaccg cgacccccgg cagtggccgg
     3841 acgcggacgt gttcaacatc tcgcggcgtg ccaccggcca cgtcggtttc gggttcggca
     3901 tccacgcctg cgtcgggatg gcgatggccc ggctggaggg cgaggccgtg ctgactgcgc
     3961 tggcccggca cgtgcggaag atcgagccga cgggcgagcc gcgccggatg ctcaacaaca
     4021 cactgcgcgg cctggagagc ctgcccctac gcctggttcc cgcgtaaccg cctgattacc
     4081 gccgctcagg gcttgcgcgt cagcaggtgt tgacacaacg atctcccgtc gtggacgtcg
     4141 tcgaattccg cccggagccc gagcagtacg cgtggacgtt cggaggggtg gcgcccaccc
     4201 accggatcac gcccggcacc gtgctgaagc tgtggacgga ggatgccttc tccggcaggt
     4261 tgcagcgcac gtccgacaag ccgtcgcagg tgctggacat gacgcaggtg aacccgcaga
     4321 ccgggccgtt ccacgtcgag ggggccgaac cgggcgacac gctcgcgctg cacatcgtcg
     4381 acctcacccc ggctagggac tgggccgcgt cgaccaccat cccgttcttc ggcgcgctca
     4441 ccggcaccga ccgcaccgcc ctgctcaacg acccgctgcc cgagcagacg tggatctacc
     4501 agctcgaccg cgaccgcggc accgtgctct tcgaggcgca gcgctcgaag ctgagcgtgg
     4561 agctaccgat cgcccccatg ctcggcaccg tcggggtggc cccgccgggg cgcgaggtgc
     4621 gttcgagcct ggtgcccgac acgttcggcg gcaacatgga cacacccgag atgcgcgccg
     4681 gcaccacctg cttcctcggg gtgaacgtcg agggcgctct gttctccgtc ggcgacgggc
     4741 actatcggca gggtgagggc gagagctgcg ggacagcggt cgagggcgcc atggacgtca
     4801 cgctcatcgt cgagctggtg aagggcgggg cgccggcgtg gccgcggctg gagcacgacg
     4861 accactacgc cgtggtcggt tcgtcgcgtc cgctggagga cgcctggcgc gccagccagg
     4921 tcgggatgat cggctggctc ggggatctgt acgggctcga cccgctcgac gcctaccagc
     4981 tgctcaccca gatcagccgg tcgccactgg ccaacgtctg cgacaccaac tacagcgcgg
     5041 tcaccaagat cgagaaggcg ctgttgcccg ccgccgacgc ctatggcggc atgcaccgcc
     5101 acctgcgcga gcaggcccgc atcctctagg aaaggatccg acaatggatc tgcagctcac
     5161 cggcaggacg gcactggtca ccggcggtac gaagggcatc ggtcgcgcag tcgtcgaggg
     5221 cctggcggcc gagggggcga gggtcgcgtt ctgcgcccgc accgacgcag acgtggcgaa
     5281 ggtcgaacag gaactgcagg ccgcgggcca cgatgtcgcc ggcaccgcgg tcgacgtggc
     5341 cgacgccgag gcgctgacgg catgggtggc cgcgtcggcg gagcgcttcg gcggcatcga
     5401 cgtcgtggtc gccaacgtca gcgcgctgtc gatcggcccc ggccctgagc actggcagcc
     5461 cagcttcgag gtcgacctca tgcacaccgt ccgcctggtg gacgcggcgc tgccccacct
     5521 ggaggcgagc ggcgtggggt cggtcatcgc ggtgtccagc gtgtccgccc gggagatcga
     5581 cttcgcgtcc ggcgcctacg gcgtgatgaa ggcggccctc gtccactaca cgtcggggct
     5641 cgccgtccag cacgccggaa aggtcagggc gaactcggtc tcccccggca acacctactt
     5701 cgagggtggg gtgtggcaga gcctcgagaa cagcgacccg gagttcttcg ccaacacgct
     5761 cagggtgaac ccgacgggtc ggctcgcgac gccggaggag atcgcctacg gcatcatcat
     5821 gctcgcgagc ccgcgtgcct cgttcatcac cggcgcccac ctggtggtcg acggcgcgct
     5881 gacccgtggg gtacagctct gagatgcgtg agttccgcgt ccagatcccc gaggcagacc
     5941 tgcaggacct gcgtggcagg ctcgcccgca cccgctggcc cgagccggcc accgcgccgg
     6001 gctggacgca gggcgttccg ctggactatg cgcgcgagct gtgcgactac tgggcccgcg
     6061 agtacgactg gcgccgctgc gaggcggagc tcaacgcgct gccgcagttc cgcaccggcc
     6121 tcgacggcgg cggcgacgac accgtcgagg tgcacctgat ccacgcccgg tcccggcacc
     6181 ccggggcgct gccgctgctg ctcagccacg gctggccggg ctcggtcgtc gagttcctcg
     6241 gcgtgatcga ggcactgacg gacccacccg acccgcgcga cgcgttccac gtcgtcgcgc
     6301 cgtcgctgcc gggctatggc ttcagcggca agccgaccgt tcccgggtgg ggtgtcgagc
     6361 ggatcgccac ggcctgggcc cagctcatgg accggctcgg ctacgaccgc tacggcgccc
     6421 agggcggcga ctgggggtcc atgatcacct cggcgctcgg caccggcgct ccgcagaacc
     6481 tcgtgggcat ccacctgacg atgccgctgg ccgcgccccc gcccgagaac gacggggtgc
     6541 ggctcacgga gtccgagcat gcggcgctcg ccgcccgtaa gaccttccag aaggtgggta
     6601 cgggctactc cagcgagcag tccacccggc cccagacgct cggctacggc ctgctcgact
     6661 ccccgtcggg tcagtgcatg tggatcgtcg agaagttctg ggactggacc gactgcgccg
     6721 gccacccgga gaacgtcatc acacgcgacc ggctgctgga caacgtgatg ctgtactggc
     6781 tgcccggcac cggcgcgtcc tcggcgcggc tgtactggga gagctacaag cggcggcgga
     6841 tggacgaggt gaccgtgccc accggcgtca ccctgttccc ccaggagctg gtccggcttc
     6901 cccggcagtg gatcgagcgc cgcttcacgg atctgcggca ctggagcgag cccgcgaccg
     6961 gcggacactt cgcgtccctg gaacagccag acgtgttcgt ggacgagctg cgcacgttct
     7021 tccgcccgct tcgctgaccc gttcccgtgc tgacctgtcc gcgggtccgg ccaccgccgg
     7081 cgagggcggc ggccgggccc accccccgac ggggccgagc tgccagaacg atcaccaccg
     7141 ggacatacct gcaatctact tgcaagaacg gcagtctgcc accggtggag ctgtcacacc
     7201 ggcggacgcg gggccaccgt gcgcagcagt aggtgcatcc cgaggtcggt cgaccgctcg
     7261 cggacgtcgg agcgtcctcc cggaagccgg ggatcccgcg cgagctcgac cccgtcaccc
     7321 gtggtcacgc agaagcagac gtaaccgacg ggcttggcct ccgtgccccc gccggggccg
     7381 gccacaccgg tgatcccgat cccgatgtcg gcaccgaacc gctcccgcgc accgtcggcc
     7441 agtgcccgtg ccacctcggg cgataccgct ccgtgctcgt cgatcatttc cgccggcacg
     7501 ccgagcagct gtgacttcgc ctcgttcgag taggccacga caccgccggc gacgtacgcg
     7561 gaggacccgg cccggtcgat caaccgcgac gcgagcatcc cgcccgtaca cgactcacca
     7621 gtcgcaacgg tccagccgcg ctccagcagc gctgcggcca gcagctcgtc ggtgctggtg
     7681 ccgtcggcac tgacgaggtg cttcgcgtgc cgggccgcga gcccggcgtg cagctccgcc
     7741 gcgacgtccg ccgcgtcggg ccggggccgc aggtccacct cgagctcgga gcggcgcaag
     7801 cacgtcgtca cctcgacgcc ggacaggtcc cgccccgcgc cgaggtcgcg cagcgtcgcc
     7861 gcgatctccg actcgggcag cccgaaga
//
LOCUS       DASRSC010000125         9510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2826348,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000125 DASRSC010000000
VERSION     DASRSC010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9510
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2826348"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1244)
                     /locus_tag="VFE39_05815"
     CDS             complement(<1..1244)
                     /locus_tag="VFE39_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003054689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6258151.1"
                     /translation="MTKPVLITVDDDPGVSRSVARDLRRRYGQDYRVVRAESGQEALE
                     ALRELTLRGEPVAAILADYRMPEMNGIEFLEQAMDVAPYARRALLTAYADTDAAIQAI
                     NLVDVDHYLLKPWDPPEEKLYPVIDALVETWRAVGVRPVEDIRVVGHRWSAECFEARD
                     FLARNSVPYRYYSIDEPEGARLLDAAGATPDDVPLLITTDGTALRSPSDAEIATACGL
                     TTDPVSDFYDLIVIGGGPAGLGSAVYGASEGLRTVLVERQATGGQAGQSSRIENYLGF
                     PDGVSGSQLTDRARRQAAKFGAEVLTARDVIGLEVRGSARVVRFGDGSEIAAHAVVLA
                     TGVSYRSLDAPGIADLTGRGVFYGSAATEAAGCSGEDIYIIGGANSAGQAAVFFAKHA
                     ATVTVLVRGPSLEASMSTYLIK"
     gene            complement(1523..2986)
                     /locus_tag="VFE39_05820"
     CDS             complement(1523..2986)
                     /locus_tag="VFE39_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase (NADP(+))"
                     /protein_id="HET6258152.1"
                     /translation="MPDTTPAELDAALAAAARAAGPLAALRPVERAALLRAAADALDA
                     AAGELVPIAIAESSLPEARLTGEVARSSGQLRLFAAALEEGSYLEVIVDTADQDAAPV
                     PRPDLRRMLVPLGPVLVFAASNFPFAFSVPGGDTASALAAGAPVVLKAHPGHPELSER
                     TGKVLVDALRAAGAPEGTFAVVHGMDAGTSALTDPRIKAAAFTGSVRGGRALLDIATS
                     RPEPIPFYGELGSLNPVFVTPAAVAARGETIADGYVSSFTLGTGQFCTKPGLLFLPEG
                     HGLEERLVEAVRGTAPAGMLNDRIRAGHAEERERLEELGPVRALVHGADAAAGVAPTL
                     LATTAKELLADPDPLLQECFGPTSIVVEYSGPEELLAAAEAFGGNLTATVHAEDADAP
                     ALPPLLDLLRERTGRLVYNGWPTGVAVAWAMHHGGPYPATTASVHTSVGATAVRRFLR
                     PICYQNTPQALLPDALRDDNPLGLPRRINGKVTRDTL"
     gene            complement(3037..3957)
                     /locus_tag="VFE39_05825"
     CDS             complement(3037..3957)
                     /locus_tag="VFE39_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET6258153.1"
                     /translation="MRTNMEIVRYIEPGARSPRVGLRRDGTVAPLPWASVAELLRESR
                     DDVRDRLTPVADEVDATDVTFLPPVDGRTEVWAAGVTYERSRTGRVEESSEASVYEKV
                     YDAERPELFLKSVAWRVVTDSEPVALRADSTLDVPEPELAVVANRFGEIVGYTVCNDM
                     SSRSIEGENPLYLPQAKIFSGHCALATGIRPAWEVPDVTDLAITLRVLRGGETVFSGD
                     TSTSRLHRTLEELVVALFAPTDFPDGAILATGTGIVPELSFSLVEGDRIEIEIAEVGT
                     LTNPVVRGREPMSWLVDALDRPDARRKAQL"
     gene            complement(4005..4157)
                     /locus_tag="VFE39_05830"
     CDS             complement(4005..4157)
                     /locus_tag="VFE39_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258154.1"
                     /translation="MLAAQGRGPQHQEPDRAVRLCRGTASEHAEPVAVGVLPEGQRRH
                     RRVSAQ"
     gene            4334..5707
                     /locus_tag="VFE39_05835"
     CDS             4334..5707
                     /locus_tag="VFE39_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6258155.1"
                     /translation="MTVLTELTDRLPSDAVLTDPASTEAYRYDMASFSPAGQPLVVVR
                     PTATEQVQQVMRIASAHGVPVVPQGARSGLSGAANAVDGCIVLSLTRMDRIVDIDPVD
                     QVAVVEPGVLNAVLSRAVLEQGLFYPPDPSSWELSTIGGNLATNAGGLCCVKYGVTAD
                     FVRGLEVVTASGELLRTGRRTAKGVAGYDLTKLIVGSEGTLGVITQATLGLRPAPEAA
                     LTMAAAFGSAADALSAVTRIMAAGLQPSLCEFLDGTTVRAIQDYRDMGLPAAAGALLL
                     TQSDRGPRAGEDVAAMGRICTELGAVDVAVASDATESAMLMEARRLVHPAMEKLGSSL
                     IDDVAVPRSRLVELLAGVEAIAEEHDVLVTCPGHVGDGNMHPTVVFDRGDPAAVERAQ
                     RAFDSIMRLGLALGGTITGEHGVGLLKRDWLATELGELSTRLHREIKAVFDPAGILNP
                     GKVLAAG"
     gene            complement(5779..6627)
                     /locus_tag="VFE39_05840"
     CDS             complement(5779..6627)
                     /locus_tag="VFE39_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003079148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6258156.1"
                     /translation="MNTATAPPTGYVHGYSEHEARRLGDQADTLAHLLHAGIAYPPGS
                     RVLEVGCGVGAQTVHLVASSPQAHVVAVDIAERSLAQARSRVDAVRPGASVELLRADL
                     HDLPFADAAFDHLFVCFVLEHLPHPEGALAALRRVLRPGGTLTVIEGDHGSAVFHPDS
                     IHARAVIDCQVRLQAHAGGNALIGRQLQPLLAGAGYIDVAVHPRTVYADRSRPELVEG
                     FTGSTFTAMVESVRDEALGTGLITPTAWDRGIADLRRTAEDGGTFHYTFFTAFAVNPV
                     GGGQAR"
     gene            6656..8068
                     /locus_tag="VFE39_05845"
     CDS             6656..8068
                     /locus_tag="VFE39_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET6258157.1"
                     /translation="MGRAKADADHRSITGTDFLQLNIADAPAGGLSDWLADRLRLAIS
                     DGRLPVGSRLPATRVLAAELRVSRGVVTEAYQRLNEDGHLAGRGRGGTVVVATPTAAP
                     LIAMPSVPAGPPAALLGAAPGEDVFDAMRAAPARIDLTPGVPDLAAFPRTAWLRAERS
                     VLADLSPSAFGYGDPRGTPALRLAVARWVARNRGIRVHPGEVVVVAGVAQALGLIAQV
                     LRRRGISEAAVEDPGSLGVRQHLQNWDLHTPPIPVDADGLRVDALRDSGAPVALLTPA
                     HQFPTGVVLAGSRRRELIAWAGQGGLIIEDDYDAEHRYDRPAVPALRSMLDEVYYAGS
                     VSKLLAPALRVGWVLAPPQYRDDIVAAKRVADLGNAALAQLVLARLMESGDLERHLRL
                     LRRRHRRRRDAMIRAIRTHLPGATVHGAAAGLHLMVTFDADLSDTDLAAAALARGVNA
                     GPARQNASHDGTDRRCDHAA"
     gene            8031..8516
                     /locus_tag="VFE39_05850"
     CDS             8031..8516
                     /locus_tag="VFE39_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005442322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HET6258158.1"
                     /translation="MMGQIVGAITLPDGTQVRGRGRREPFPPGPFPAYGLYLGRSPDT
                     PGRRFLGRRKPAWRPDWPADWIDWPDFRTPRDHQAAAALIQHAYSMAQSGEQIEVACV
                     GGTGRTGTVIACMAVLAGHPADDAVRWTRQHYRRHAVETRSQRRWITWFATQYLADAR
                     P"
     gene            complement(8526..>9510)
                     /locus_tag="VFE39_05855"
     CDS             complement(8526..>9510)
                     /locus_tag="VFE39_05855"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009027 - tartrate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0046395 - carboxylic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HET6258159.1"
                     /translation="TVLDAVCARHDIALAYTEFDWSCQRFEREGAMMPPDGIDTLRGF
                     DAILLGAVGWPGVPDHVSLWGLLIPIRRAFRQYANLRPIRVFDGVQSPLRGAEDVDFV
                     VVRENVEGEYSEVGGRANRGFPDEMAVQESIFTRVGVSRIADFAFELAQTRRGYLTSA
                     TKSNGIVHTLPFWDEVVAERAARYPDVRWDSEHIDALAAKFVLQPGRFDVVVGSNLFG
                     DILSDLAAAVAGSIGIAPSANLDPTKEFPSMFEPVHGSAPDIAGQGVANPVGAVWSAA
                     LMLEHLGHAAAAADVLAAMEATLAKPETRTADLGGTATTAQVTAALVGHLS"
BASE COUNT         1306 a         3450 c         3459 g         1295 t
ORIGIN      
        1 tgcttgatca ggtaggtcga catcgacgcc tccagactcg gcccgcgcac cagcaccgtg
       61 acggtggctg cgtgcttcgc gaagaacacc gcagcctgcc cggcggagtt ggcgccgccg
      121 atgatgtaga tgtcctcgcc gctgcagcct gccgcctccg tggcggcgga gccgtagaac
      181 acgccgcggc cagtgaggtc ggcgatgccc ggcgcgtcca ggctccggta ggacacaccc
      241 gtggcgagca cgacggcgtg cgccgcgatc tcactgccgt cgccgaaccg cacgacgcgg
      301 gccgacccgc gcacctccag cccgatgacg tcgcgggcgg tgagcacctc ggccccgaac
      361 ttcgcagcct gtcgccgcgc ccggtcggtg agctggctgc ccgagacgcc atcggggaag
      421 cccaggtagt tctcgatccg tgagctctgc ccggcctggc caccggtggc ctgccgctcc
      481 accagcacgg tgcgcagccc ctctgacgcg ccgtagacgg ccgagcccag cccggccggg
      541 ccgccgccga tcacgatgag gtcgtagaag tccgacaccg ggtcggtggt gagcccgcag
      601 gctgtggcga tctcggcgtc cgacggcgac cgcagcgccg tgccgtcggt ggtgatcagc
      661 aggggcacgt cgtcgggcgt ggcgcctgcg gcatcgagga gccgggcacc ctccggctcg
      721 tcgatggagt agtagcggta gggcaccgag ttgcgggcca ggaagtcgcg ggcctcgaag
      781 cattccgccg accaccggtg cccgacgacg cggatgtcct cgacgggccg cacgcccacg
      841 gcgcgccacg tctccaccag agcgtcgatc accgggtaga gcttctcctc cggcgggtcc
      901 cacggcttga gcaggtagtg gtcgacgtcg acgaggttga tcgcctggat cgcggcgtcg
      961 gtgtcggcgt aggccgtgag cagcgcccgg cgcgcgtacg gcgccacgtc catggcctgc
     1021 tcgaggaact cgatgccgtt catctccggc atccggtagt cggccaggat cgccgcgacg
     1081 ggctccccgc ggagcgtcag ctcccgcagc gcctccagcg cctcctggcc ggactccgcg
     1141 cgcaccacgc ggtagtcctg gccgtagcgg cggcgcaggt cgcgagccac ggaacggctc
     1201 actccagggt cgtcgtcgac ggttatgagg acgggcttgg tcatggggct cctgccgaac
     1261 gtgccattcc gtcgatattg cccccacgcg ccgatgcgcg gtgctgctgg cgtccgccaa
     1321 cggcgaactc acaactcgaa cctagcccgc agacgcctcc ggccgcgagg cctcacccgg
     1381 ctgctgctcc gggtggcggc tccaggatcg gcgcgtgggg gagccggtgg tccccgcgcc
     1441 gcgccccttg gacgaacggc actctgggcc ggctctaccg gccgaaagtg cgttcgctcg
     1501 gcgctcgggg tccggccgcg cctcagagcg tgtctcgggt caccttccca ttgattcgtc
     1561 tcggcaggcc gagggggttg tcgtcccgca gcgcgtccgg gagcaacgcc tggggcgtgt
     1621 tctggtagca gatcgggcgc aggaagcggc gcacggcggt ggcacccacc gaggtgtgca
     1681 ccgaggccgt ggtggccggg tacgggccgc cgtggtgcat cgcccaggcc actgccaccc
     1741 ccgtgggcca gccgttgtag acgagcctgc cggtgcgctc gcgcagcagg tcgagcagcg
     1801 gcggcaacgc cggcgcgtcg gcgtcctcgg cgtggacggt ggccgtcaga ttgcccccga
     1861 acgcctccgc agcggccagc agctcctcgg gcccgctgta ctcgaccacg atcgacgtgg
     1921 gtccgaagca ctcctgcagc agcgggtccg ggtcagcgag cagctccttg gcggtggtgg
     1981 ccagcagcgt cggggccacc ccggctgcgg cgtcggcccc gtggacgagg gcgcgtacgg
     2041 ggccgagctc ctccagccgc tcgcgctcct ccgcgtgccc ggcacggatg cggtcgttga
     2101 gcatcccggc cggcgccgtg ccacggacgg cctcgacgag ccgctcctcc aacccgtgtc
     2161 cctccggcag gaacagcagg ccgggcttcg tgcagaactg cccggtgccg agggtgaacg
     2221 atgacacgta gccgtcggcg atggtctcgc cgcgggcggc gaccgcggcg ggcgtgacga
     2281 acaccgggtt caggctgccc agctcgccgt agaacgggat cggctccggg cgggaggtgg
     2341 cgatgtcgag cagggcacga ccgccgcgta ccgagccggt gaacgctgcc gccttgatcc
     2401 gcgggtcggt cagcgccgag gtgccggcgt ccatgccgtg caccaccgcg aacgtcccct
     2461 cgggtgcgcc cgcggcgcgc agcgcgtcga cgagcacctt ccccgtgcgc tcggacagct
     2521 ccgggtggcc ggggtgggcc ttgagcacca cgggagcacc tgccgcgagc gccgaggcgg
     2581 tgtcgccgcc gggaacgctg aacgcgaacg ggaagttgct tgcggcgaac accagcaccg
     2641 ggccgagcgg caccagcatg cggcgcaggt ccggccgcgg gacgggtgcg gcgtcctggt
     2701 cggccgtatc gacgatcacc tcgaggtagg agccctcctc gagcgcggcg gcgaacagcc
     2761 gtagctggcc gctggagcgg gccacctcgc cggtcagccg cgcctccggc agcgacgact
     2821 ccgcgatggc gatcgggacg agctcgcctg cggcggcgtc gagcgcgtcg gcagcggcgc
     2881 gcagcagcgc ggcacgctcg acgggacgca gtgcggccag cgggcccgct gcacgggcgg
     2941 ctgcggcgag ggcggcgtcg agctccgctg gggtggtgtc gggcacggcg tggtcctcaa
     3001 cagcgttgtt cccagcggtg gtgttcccag cggcgttcac agctgcgcct tcctcctggc
     3061 atcggggcgg tcgagggcgt ccacgagcca cgacatcggt tcgcggccgc ggacgacggg
     3121 gttggtgagg gtgccgacct cggcgatctc gatctcgatc cggtcgccct cgacgagcga
     3181 gaagctcagc tccgggacga ttccggtgcc ggtggcgagg atcgccccgt cggggaagtc
     3241 ggtgggcgcg aagagcgcca cgacgagctc ctccagggtc cggtgcaggc gggacgtgct
     3301 ggtgtcgccg ctgaacacgg tctctccccc gcgcagcacc cgcagcgtga tggccaggtc
     3361 ggtgacgtca ggcacctccc aggccggccg gatgccggtg gccagcgcgc agtggccgct
     3421 gaagatcttc gcctgcggga ggtagagggg gttctcgccc tcgatcgaac gggagctcat
     3481 gtcgttgcag accgtgtagc cgacgatctc cccgaagcgg ttggcgacca ccgcgagctc
     3541 cggctccggg acgtcgaggg tggagtcggc gcgcagggcg accggctcgg agtcggtgac
     3601 gacccgccag gccaccgact tgagaaacag ctcggggcgc tccgcgtcgt acaccttctc
     3661 gtacaccgag gcctcgctgc tctcctcgac ccggccggtg cgcgagcgtt cgtaggtgac
     3721 acctgccgcc cacacctcgg tgcggccgtc cacgggaggc aggaacgtga cgtcggtggc
     3781 gtccacctcg tcggccacgg gggtgagacg gtcgcgcacg tcgtcgcggg actcacgcaa
     3841 cagctcggcg acactggccc agggcaacgg cgcgacggtg ccgtcgcggc gcagcccgac
     3901 gcgtgggctc cgcgctcccg gctcgatgta gcggacgatc tccatattcg tgcgcactcc
     3961 catcctgggc gcgggccctg ttgacccccg aacgggagcg aatcctattg cgcggacacc
     4021 cggcgatgcc gccgctgacc ctcgggcagc acgccgacgg cgaccggctc cgcgtgctcg
     4081 gacgcggtgc cccggcacag ccgcaccgcg cggtccggct cctggtgctg cggcccgcgc
     4141 ccctgggcag cgagcatgtg cggaaaccga gtgtcccagg tagaccgcga ggccggcccg
     4201 ttccctggcc ggtccctgag tcgctcgtca catgtgacgc agccggtcgg agcgcgcgtg
     4261 gcagggcagg cacccggact ctcactacga gcggctgcgt cccacatccg acgacccgac
     4321 cgggaggaac agcatgaccg tgctcaccga actgaccgac cggttgccgt ccgacgccgt
     4381 cctgacggac ccggcaagca cggaggcgta ccggtacgac atggcgagct tctctccagc
     4441 cggacaaccc ctcgtcgtgg tgcggccgac agccacggag caggttcagc aggtgatgcg
     4501 gatcgcgtcg gcgcacggcg taccggtggt gccgcagggc gcgcggtcgg ggctgtcggg
     4561 agccgccaat gccgtggatg gctgcatcgt gctgtcgctg acccggatgg accgcatcgt
     4621 cgacatcgac cccgtcgacc aggtcgcggt ggtcgagccg ggcgtgctca acgcggtcct
     4681 ctcgcgcgcg gtgctcgagc aggggctctt ctacccgccc gacccgtcat cgtgggagct
     4741 ctcgacgatc ggcggcaacc tcgcgacgaa cgcgggcgga ctgtgctgcg tgaagtacgg
     4801 ggtcaccgcc gacttcgtcc gcggcctcga ggtggtgacg gcgagcgggg agctgctgcg
     4861 cacggggcgc cgcaccgcga agggcgtggc gggctacgac ctgacgaagc tgatcgtcgg
     4921 ctctgagggc acgctcggcg tcatcaccca ggcgacgctc gggctgcgcc cggcgccgga
     4981 agccgcgctg acgatggccg ccgcgttcgg ctcggccgcc gacgcgctca gcgccgtcac
     5041 ccggatcatg gccgcgggcc tgcagccgtc gctgtgcgag ttcctcgacg gcaccaccgt
     5101 gcgggcgatc caggactacc gcgacatggg actcccggcc gctgcgggcg cgttgttgct
     5161 cacccagtcc gaccggggtc cgcgcgcggg cgaggacgtc gcagcgatgg gccggatctg
     5221 cacggagctg ggcgcggtcg acgtcgcagt ggcctcggac gccacggagt cggcgatgct
     5281 catggaagct cgccggctcg tccaccccgc catggagaag ctcggctcct cgctcatcga
     5341 cgacgtggcc gttccgcggt cacgcctggt cgagctgctc gcgggggtcg aggcgatcgc
     5401 cgaggagcac gacgtgctgg tgacctgccc ggggcacgtg ggagacggca acatgcaccc
     5461 gacggtcgtc ttcgaccggg gcgatcccgc ggccgtcgaa cgggcgcagc gcgcgttcga
     5521 ctcgatcatg cgcctgggcc tggcgctggg cggcaccatc accggcgagc acggtgtcgg
     5581 tctcctcaag cgggactggc tggccaccga gctgggcgag ctcagcaccc gcctgcaccg
     5641 cgagatcaag gcggtgttcg atcccgcggg catcctgaac ccgggaaagg tgctggccgc
     5701 cggctgagct gcgggcagcg catggtcatc ggcgcgacat cgcacagctg ccttctgcgc
     5761 gaccgcgacg cacccgcgtc aacgcgcctg cccgccgccc accgggttca cggcgaacgc
     5821 ggtgaagaac gtgtagtgga aggtcccgcc gtcctcggcg gtccggcgca ggtcggcgat
     5881 cccccggtcc cacgcggtcg gggtgatcag gccggtgccc agggcctcgt cccgcaccga
     5941 ctcgaccatc gccgtgaagg tgctgccggt gaagccctcg accagctccg gccggctccg
     6001 atcggcgtac acggtccgcg ggtgcacggc cacgtcgatg tacccggcgc cggccagcag
     6061 cggctgcagc tgccggccga tcagggcgtt gccgccggcg tgggcctgca gccggacctg
     6121 gcagtcgatc acggctcggg catggatgct gtcggggtgg aacaccgcgg agccgtggtc
     6181 gccctcgatc acggtgaggg tgccgccggg tcgcagcacg cgccgcagcg cagccagcgc
     6241 tccttccggg tgcggcaggt gctccagcac gaagcagacg aagaggtggt cgaacgcggc
     6301 gtcggcgaag ggcaggtcgt gcaggtccgc ccggagcaac tcgacgctcg ccccggggcg
     6361 gacggcgtct acgcgggagc gggcctgcgc gagggagcgc tctgcgatgt ccaccgccac
     6421 gacgtgcgcc tgcgggctgg acgcgaccaa atgcacggtc tgcgcgccga caccgcatcc
     6481 gacctccagt acccgactcc caggggggta ggcgatgccg gcgtgcagca ggtgggcgag
     6541 cgtgtcggcc tggtcgccca gccggcgggc ctcgtgctcc gagtacccgt ggacgtagcc
     6601 ggtggggggc gcggtcgcgg tgttcatatc tccaccatgc tgcgactatg gcccgatggg
     6661 cagggccaaa gccgacgctg accaccggtc cataacggga accgatttcc tgcagctgaa
     6721 catcgcggac gcccctgccg gcgggctgtc cgactggctg gcagaccggc ttcggctcgc
     6781 gatctcggac ggacggctgc cggtgggcag caggctcccc gccacgcggg tgttggcggc
     6841 ggagctgcgg gtgtcgcgcg gtgtcgtcac cgaggcctac caacgcctca acgaggacgg
     6901 ccacctcgca ggccgtggac ggggcgggac tgtcgtggtc gccactccaa cggcggcgcc
     6961 attgatcgca atgccgtccg ttcccgctgg tcccccggcc gcactgctcg gcgcggcccc
     7021 cggcgaggac gtcttcgacg cgatgcgcgc agcgcccgct cggatcgacc tgacacccgg
     7081 cgtacccgac ctggccgcgt tcccgcggac ggcgtggcta cgcgccgaac gatccgtgct
     7141 ggccgacctc tcgccgtccg ccttcgggta cggggacccg cgcgggacgc ccgcgctgcg
     7201 gctcgccgtc gcgcgctggg tggcccgcaa ccgcgggatc agggtgcatc ccggcgaggt
     7261 ggtcgtggtt gcgggcgtcg cgcaagcgct ggggctgatc gcgcaggtgc tgcgcaggcg
     7321 cgggatctcc gaggcggcgg tcgaggaccc cgggtcgctc ggtgtccgcc agcacctgca
     7381 gaactgggac ctgcacaccc cgccgatacc ggtggacgca gacggcctcc gcgtcgacgc
     7441 gctgcgcgac agcggcgccc ccgtagctct gctcaccccg gcacaccagt tcccgacagg
     7501 agtggtgctc gcgggcagca ggcgccgcga gctcatcgcg tgggccggcc agggcggcct
     7561 gatcatcgag gacgactacg acgccgagca ccgctacgac cggcccgcgg tgcccgcact
     7621 gcggtcgatg ctcgacgagg tctactacgc cggcagcgtc tccaagctcc tcgccccggc
     7681 gttacgggtg ggatgggtcc tcgcgccgcc gcagtaccgg gacgacatcg tcgcggccaa
     7741 gcgcgtcgcc gacctcggca acgccgcact ggcgcagctc gtgctggccc ggctgatgga
     7801 gtcgggcgat ctggagcgcc acctgcgact gctgcgccga cgtcaccgcc gcagacgaga
     7861 cgcgatgatc cgcgcgatcc ggacacacct gccgggcgcc accgtgcacg gcgccgcagc
     7921 gggtttgcac ctgatggtca cgttcgacgc cgacctctcc gacaccgacc tcgccgcagc
     7981 ggcattggcc cggggtgtga acgccggccc tgccaggcag aatgcctcgc atgatgggac
     8041 agatcgtcgg tgcgatcacg ctgcctgacg gaacccaggt gaggggccgc ggccgccgcg
     8101 agcccttccc gcccggtccc ttccccgcgt acggcctcta tctgggccgc tcccccgaca
     8161 cgccggggcg gcggttcctc gggcgccgca agcccgcgtg gcgcccggac tggcccgccg
     8221 actggatcga ctggcccgac ttccggaccc cccgcgacca ccaggcggcc gccgcgctga
     8281 tccagcacgc ctactcgatg gcccagtcag gggagcagat cgaggtggcg tgcgtgggcg
     8341 ggacgggccg cacgggcacg gtgatcgcct gcatggcggt gctcgccggt cacccggccg
     8401 acgacgccgt ccggtggacc cggcagcact accggcgaca cgcggtcgag acgcgcagcc
     8461 agcgacgctg gatcacgtgg ttcgccacgc agtacctcgc agacgccagg ccctgacgaa
     8521 accggtcagc tcaggtgccc caccagcgcc gccgtcacct gcgcggtcgt cgccgtgccg
     8581 ccgaggtcgg ccgtgcgggt ctccggcttc gccaacgtgg cctccatggc cgcgaggacg
     8641 tcggcggccg cggccgcgtg gccgaggtgc tccagcatca gcgcagccga ccacacggct
     8701 cctaccgggt tggccacgcc ctgccccgcg atgtccggcg ccgagccgtg caccggctcg
     8761 aacatggagg ggaactcctt cgtcgggtcc aggttcgccg acggcgcgat cccgatcgag
     8821 ccggccacgg cggcggcgag gtcggagagg atgtcgccga acaggttcga gccgaccacg
     8881 acgtcgaacc gcccgggctg cagcacgaac ttcgcggcga gcgcgtcgat gtgctcgctg
     8941 tcccaccgca catccgggta ccgggctgcc cgctcggcca cgacctcgtc ccagaacggg
     9001 agtgtgtgca cgatcccgtt cgacttcgtc gccgacgtga ggtacccgcg cctcgtctgc
     9061 gccagttcga acgcgaagtc ggcgatccgg ctcaccccga cccgggtgaa gatcgactcc
     9121 tggacggcca tctcgtccgg gaacccccgg ttggcccggc caccgacctc gctgtactca
     9181 ccctcgacgt tctcgcggac gacgacgaag tcgacgtcct cagcgccgcg cagcgggctc
     9241 tgcacgccgt cgaacacccg gatcgggcgg aggttggcgt actgccggaa cgcacgccgg
     9301 atcgggatca gcagtcccca cagcgacacg tggtcgggca cccccggcca gcccacggcg
     9361 ccgagcagga tcgcgtcgaa accacggagc gtgtcgattc cgtcgggcgg catcatcgcg
     9421 ccctcgcgtt cgaagcgctg acacgaccag tcgaactcgg tgtacgcgag cgcgatgtcg
     9481 tgccgggcgc acacggcgtc cagcaccgtc
//
LOCUS       DASRSC010000126         8501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1352176,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000126 DASRSC010000000
VERSION     DASRSC010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8501
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1352176"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..595
                     /locus_tag="VFE39_05860"
     CDS             <1..595
                     /locus_tag="VFE39_05860"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET6258160.1"
                     /translation="GEYDGSQNVVSNASCTTNCLAPLAKVIDDLLGIEKGLMTTIHAY
                     TQDQNLQDGPHKDLRRARAAAINLVPTSTGAAKAIGLVLPHLKGKLDGYAVRVPIPTG
                     SATDLTVEVRKEATVEEINAAYRAAAAEGPLKGILTYTEDPIVSSDIVTDPASCIFDS
                     GLTKVIGNQVKIVGWYDNEWGYSNRLVDITALVASKL"
     gene            603..1805
                     /locus_tag="VFE39_05865"
     CDS             603..1805
                     /locus_tag="VFE39_05865"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET6258161.1"
                     /translation="MKTLGDLVAVLDEDLFGRRVLVRADLNVPLDGSRITDDGRIRAS
                     VPTLARLRETGTRVVVVAHLGRPKGAPDPAFSLGPVAERLGELLGVQVPLADLGDEAR
                     FTVDALPEGGVALLENIRFDPRETSKNDDERASLARELAALVGPEGVFVSDGFGVVHR
                     KQASVYDVAQDLPSYAGDLVLAEVEVLRTLTESPKRPYAVVLGGSKVSDKLAVIEALL
                     PKVDALLIGGGMCYTFLAAHGYGVGDSLLERDQIGTCRKLLESGKIVLPSDVVVADDF
                     SAEANTRTVAVDAIPDGWKGLDIGPESVTSFGEVLEGAATVFWNGPMGVFELAPFVDG
                     TRGVAQAIVDATAGGAFSVVGGGDSAAAVRALGLAEDGFSHISTGGGASLEYLEGKTL
                     PGLAVLEQ"
     gene            1818..2636
                     /gene="tpiA"
                     /locus_tag="VFE39_05870"
     CDS             1818..2636
                     /gene="tpiA"
                     /locus_tag="VFE39_05870"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET6258162.1"
                     /translation="MASGTDNPSAGARRKPFIAGNWKMNCNHLEALALVQKIAFALPE
                     KYYDKVEVAVLPPFTDIRSVQTLIDGDNLLMVHGAQDLSPYDSGAYTGDVSGSMLAKL
                     GCRYVVVGHSERREIHGEDDALVNAKVHAAIRHGIVPILCVGEGLEVREAGDHVAHTT
                     QQVTAALEKVQSQHAQTLVIAYEPVWAIGTGRVASAADAQEVCAAIRESVSAKYGQAI
                     GASLRVLYGGSVKAKNVGEIVAEPDVDGALVGGASLNADEFAQLCAIAAGGPLP"
     gene            2840..3076
                     /gene="secG"
                     /locus_tag="VFE39_05875"
     CDS             2840..3076
                     /gene="secG"
                     /locus_tag="VFE39_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET6258163.1"
                     /translation="MQLALQIVLILSSVVLVLLILLHRGRGGGLSSLFGGGVQSSLSG
                     SSVVEKNLDRLTLFVGAIWVIAIIGTGLLVKLGI"
     gene            3089..3430
                     /locus_tag="VFE39_05880"
     CDS             3089..3430
                     /locus_tag="VFE39_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HET6258164.1"
                     /translation="MAGGNAIRGTRVGAGPMGESERGETAPRSRISYFCSNGHITTPS
                     FASEADIPDSWDCPRCGLPAGRDGENPPAPPRTEPYKTHLAYVKERRSDADGAAILEE
                     ALGRLRASREL"
     gene            complement(3480..4031)
                     /locus_tag="VFE39_05885"
     CDS             complement(3480..4031)
                     /locus_tag="VFE39_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258165.1"
                     /translation="MADGRGARSGVIAGRVAAALGAVALLAGCSVGSGPAEPEPAVSP
                     PPPPPPAACVLDTAALGAATGLTWTPDETTASDTRCVYDPAGATSNPDGPAFVAVDIA
                     PAGGPDAGSTLDTVAGLCDPNSRADVTAADGGFVCRFQGGSVFAAAVRNEQVVTVAAS
                     AVPPGTTAARIVTAFSEQLRALR"
     gene            4234..4398
                     /locus_tag="VFE39_05890"
     CDS             4234..4398
                     /locus_tag="VFE39_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258166.1"
                     /translation="MPHPAAIRARIDEALAAKETGAERVILFNYCGHGFPDLSAYDDY
                     LNGRLPDEQG"
     gene            complement(4428..4880)
                     /locus_tag="VFE39_05895"
     CDS             complement(4428..4880)
                     /locus_tag="VFE39_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6258167.1"
                     /translation="MTLDLPTPAPTPAEHQGAVAFFTARLSYQTDVADVRAALAGDRP
                     GFVLVDSRGGAAWRQGRIPGALHLPTDEIPGRAPHLLDPAMPLVTYCWGPGCNGATRA
                     ALTLSRLGYRVREMIGGIEYWIREGFPVETATGTTAAAPDPLTAPCGC"
     gene            5017..5535
                     /locus_tag="VFE39_05900"
     CDS             5017..5535
                     /locus_tag="VFE39_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007033885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET6258168.1"
                     /translation="MSVESLDDVDWRLLEALQRDGRASYADLGRLVGLSPSAVTERVR
                     RLEESGVITGYTAEVNPEKLGLPITALVRLRYPHGNYKPFRDLLAATPEITEAHHVTG
                     EDCFVLTVRTRSMRHLEEVTGRISGLGAVTTSVVYSSPLPRRSRRHGDGRRHYRGEVL
                     PPQELSSHSADS"
     gene            complement(5552..5986)
                     /locus_tag="VFE39_05905"
     CDS             complement(5552..5986)
                     /locus_tag="VFE39_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258169.1"
                     /translation="MSTPEVPMLHVIVDGANVVGSRPNGWWRDRPGAARRLAGKLSAA
                     LTAGPARLAEALGREASDEDVMVHLVLEGAAARVEDLPTHPQLDVVRAPADGDTAIAA
                     LAADLASEQVLVVTADRELRERVRSTGAETIGPGTLLRALPG"
     gene            complement(6009..6788)
                     /gene="pgl"
                     /locus_tag="VFE39_05910"
     CDS             complement(6009..6788)
                     /gene="pgl"
                     /locus_tag="VFE39_05910"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HET6258170.1"
                     /translation="MATPDIAVHSHPNVLAAAAAARLVTRLVDLQAAGNTPKIVLTGG
                     STGIAVLEQIRASVARDAVDWSRVEFYWGDERFVPPDDPERNEKQAREALLDHIPVDP
                     AKIFPMGADTGTGPGGAEAAAEEYAAVLARAARPEDHGPVPSFDVLMLGMGEEGHTAS
                     VFPHSPAVYETERPVVAVHGCPKPPPTRVTLTLPAIRRAAEVWIMTTGESKAAAVAMA
                     LGGAGEVAIPVAGARGRRRTLWLLDRAAASKLPRDLVPPIA"
     gene            complement(6788..7753)
                     /gene="opcA"
                     /locus_tag="VFE39_05915"
     CDS             complement(6788..7753)
                     /gene="opcA"
                     /locus_tag="VFE39_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HET6258171.1"
                     /translation="MIVDLPSSTTSAVNKKLVELRESNGVLALGRVLTLVILTDDGSA
                     IEGAIEAANSASREHPCRVIVLARGQRKAAPRLDAQIRVGGDAGASEVIVLRGYGALA
                     ADEAGAGMVMPLLLPDAPVVAWWPGEAPAVPSQDAVGRLAQRRITDALSSKNPLRAFE
                     QRRANHVAGDTDLTWTRLTAWRALLATALDAPPHEDVTGATVAGEAVSPSTDLLAGWL
                     AVRLDAKVKRSPALAGPGLVSVRLDRRSGPIELIRPDGKVGSLRQPGQPDRLVALARR
                     EVRDCLAEELRRLDPDEIYGAALAGVSLIARGRTPVKVPALTAGS"
     gene            complement(7785..>8501)
                     /locus_tag="VFE39_05920"
     CDS             complement(7785..>8501)
                     /locus_tag="VFE39_05920"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET6258172.1"
                     /translation="MDEPLSFEPKSLRAEKAKVLAATKPLEPFEQTTARGQYAGGWQG
                     GTKVPGLLQEEGFAKDSKTETYAAVTLEVQSRRWAVVPFYLRTGKRLGRRVTEIAVIF
                     KRAPHLPFDATMTEELGQNAFVIRVQPDEGVTMRFGSKVPGSQMEVRDVTMDFGYGAA
                     FTEASPEAYERLILDVLLGEPSLFPVNEEVERSWEILDPVLEHWAAQEEGPDPYPAGS
                     WGPESANAMLARTGRTWRRP"
BASE COUNT         1191 a         3016 c         3076 g         1218 t
ORIGIN      
        1 cggggagtac gacggctcgc agaatgtcgt gtcgaatgcg tcctgcacca cgaactgcct
       61 cgccccgctg gcgaaggtca tcgacgacct gctcgggatc gagaagggtc tgatgaccac
      121 gatccacgcc tacacccagg accagaacct gcaggacggc ccgcacaagg acctgcgccg
      181 cgcccgggct gccgcgatca acctcgtgcc cacctcgacc ggcgccgcga aggccatcgg
      241 cctggtgctg ccgcacctca agggcaagct cgacggttac gcggtgcggg tgccgatccc
      301 gacgggctcg gccaccgacc tgactgtgga ggtccgcaag gaggccacgg tcgaggagat
      361 caacgcggcg taccgggcgg cggccgcgga gggcccgctc aagggcatcc tgacctacac
      421 cgaggacccg attgtctctt ccgacatcgt caccgacccg gcgtcgtgca tcttcgactc
      481 tggcctgacg aaggtgatcg gcaaccaggt caagatcgtc ggttggtacg acaacgagtg
      541 gggatactcc aaccgcctgg tcgacatcac ggcgctggtc gcgtcgaagc tgtagcggcg
      601 ccgtgaagac tcttggcgac ctcgtcgcag ttctcgacga ggacctgttc ggtcgtcggg
      661 tactcgtccg ggccgacctc aacgtgcccc tcgacggctc gaggatcacc gacgacggcc
      721 ggatccgggc ctccgtcccc acgctggcgc gcctgcgtga gaccggcacc cgggtcgtgg
      781 tcgtcgccca cctcggccgg ccgaagggtg cacccgaccc ggcgttctcc ctgggacctg
      841 tcgccgagcg gctcggcgaa ctcctcggcg tgcaggtgcc gctggccgac ctcggcgacg
      901 aggcccggtt caccgtcgac gcgctgcccg agggcggcgt cgcgctgctt gagaacattc
      961 ggttcgaccc tcgtgagacg tcgaagaacg acgacgagcg ggcgtcactg gcccgtgagc
     1021 tcgccgcgct ggtcggtccc gaaggtgtgt tcgtctccga cgggttcggc gtcgtgcacc
     1081 gcaagcaggc gtcggtctac gacgtggccc aggacctccc ctcctacgcg ggagacctcg
     1141 tgcttgccga ggtcgaggtc ctgcgcaccc tcaccgagtc gccgaagcgg ccctacgcgg
     1201 tggtgctcgg gggctccaag gtctccgaca agcttgccgt gatcgaggcg ctgctgccga
     1261 aggtcgacgc gctgctcatc ggcggcggga tgtgctacac gttcctggcc gcccacggct
     1321 acggggtcgg cgactcgttg ctggagcgcg accagatcgg cacctgccgc aagctcctcg
     1381 agtcgggcaa gatcgtcctg ccgtcggacg tcgtggtggc cgacgacttc tcggccgagg
     1441 cgaacacgcg gaccgtcgcc gtcgacgcga tccccgacgg ctggaagggt ctcgacatcg
     1501 gccccgagtc ggtcacgtcg ttcggggagg tgctcgaggg agcggccacg gtgttctgga
     1561 acgggcccat gggcgtgttc gagctcgcac cgttcgtcga cggcacccgg ggggtggcgc
     1621 aggcgatcgt cgatgccact gccggcgggg ccttcagcgt ggtcggcggc ggcgactccg
     1681 cggcggcggt gcgggcgctg ggcctggccg aggacgggtt ctcgcacatc tcgaccggcg
     1741 gcggggcgtc gctggagtac ctggagggca agacgctccc cgggctcgcc gtactggagc
     1801 agtagatggc acgtggcatg gcaagcggca ctgacaaccc ctccgccggc gcccgccgga
     1861 agccgttcat cgcgggcaac tggaagatga actgcaacca tctcgaggct ctcgcgctgg
     1921 tgcagaagat cgcgttcgcg ctgccggaga agtactacga caaggtcgag gtggcggtgc
     1981 tgccgccgtt caccgacatc cgcagcgtcc agacgctgat cgacggcgac aacctgctga
     2041 tggtgcacgg ggctcaggac ctctccccgt acgactcggg cgcctacacg ggcgacgtgt
     2101 ccgggtcgat gctcgcgaag ctcggctgcc gctacgtcgt cgtcgggcac tcggagcgcc
     2161 gggagatcca cggcgaggac gatgcgctcg tcaacgccaa ggtgcatgcc gcgatcaggc
     2221 acggcatcgt tccgatcctg tgtgtcggcg agggtctcga ggtccgcgag gcgggcgacc
     2281 acgtcgccca caccacccag caggtcaccg cggcgctgga gaaggtgcag agccagcacg
     2341 cccagaccct cgtgatcgcc tacgagccgg tgtgggcgat cggcaccggg cgcgtcgcga
     2401 gcgcggccga cgcccaggag gtctgcgccg ccatccgcga gtcggtgtcg gcgaagtacg
     2461 gccaggcgat cggggcgagc ctgcgggtgc tctacggcgg ctcggtcaag gcgaagaacg
     2521 tcggcgagat cgttgccgag cccgatgtgg acggtgcgct cgtgggcggg gcgagcctga
     2581 acgccgacga gttcgcgcag ctgtgcgcga tcgccgcggg cgggccgctc ccgtgacacc
     2641 gcactccgtg atgaccggcc gctgcgctcg cgcgctgtcg acgatcggtc ggtagccgtc
     2701 ggtgagcggg tggtgaccga gcgggcgcgg ccagccgtgt gaccccggac aacctgcgga
     2761 tcagggccag gtacgctcga gatcccgagc ggtgtggtgc gccgtcgccg gtgctcggct
     2821 cgatgagaat gaggatggga tgcaactcgc cctgcagatc gtactgatcc tctccagcgt
     2881 ggtgctggtg ttgctgatcc tgctgcaccg cggtcgcggt ggtggcctct cgagcctgtt
     2941 cggtggcggc gtgcagtcca gtctctccgg gtcgagcgtg gtggagaaga acctcgaccg
     3001 gctcaccctt ttcgtcggcg cgatctgggt gatcgccatc atcggaaccg gtctgctcgt
     3061 gaagctcggg atctgaggag gaatggccat ggctggcggc aacgcgatcc gcggcacccg
     3121 ggtgggtgcc ggtcccatgg gcgagtccga gcggggcgag accgcgcccc ggtcgcgcat
     3181 ctcgtacttc tgctcgaacg ggcacatcac caccccgtcc ttcgcctccg aggcggacat
     3241 cccggactcc tgggactgcc ctcgctgcgg gctccccgca gggcgcgacg gtgagaaccc
     3301 gccggctccg ccgcgcaccg agccctacaa gacgcatctc gcctacgtga aggagcggcg
     3361 cagcgacgcc gacggtgccg cgatcctcga ggaggcgctc ggcaggctgc gggcatcgcg
     3421 cgagctgtag cggcaggagg caggggccgc agcgtccctg atcaccgcag cgtccctgat
     3481 caccgcagtg ccctgagctg ttcgctgaac gccgtcacga tgcgcgcggc ggtggtgccg
     3541 ggaggcaccg cggaggcggc gaccgtgacg acctgttcgt tacgcacggc tgcagcgaac
     3601 acgctgccgc cctggaaccg gcagacgaaa ccgccatccg ccgccgtcac gtcagcccgc
     3661 gagttcgggt cgcacaggcc ggccaccgtg tcgagcgtgg agcccgcgtc cgggccgccg
     3721 gccggtgcga tgtcgacggc gacgaaggcg ggcccgtccg gattggacgt ggcgccggca
     3781 gggtcgtaca cgcaccgggt gtcgctcgcc gtcgtctcgt cgggcgtcca ggtcaggccg
     3841 gtggcggcgc cgagcgccgc ggtgtcgagc acgcacgccg caggcggggg cggcggagga
     3901 ggcgaaacgg ccggctctgg ttcggccggg ccggagccga cggagcatcc cgcgagcagc
     3961 gccaccgcgc ccaatgccgc ggcaacacgg cctgcgatca cccctgaccg tgcaccccgt
     4021 ccgtccgcca cgccgtgcac cttcccacgc tgcccctgtc agctgtgcgg cagcgctccc
     4081 cgtggtcatc gcactccgcg gcggtgggtc gaacctgggc gccatcgcgt tccccttcgt
     4141 gcctgacgcc ggcgtccggc tgctcgccgt cgagccgtcg tcgtgcccga cgctcacccg
     4201 tggccggttc gagtacgact tcggcgacac ggcgtgcctc accccgctgc catccgggcc
     4261 aggatcgacg aggccctcgc cgcgaaggag accggtgcgg agcgggtgat cctgttcaac
     4321 tactgcggtc acggcttccc tgacctgtcc gcgtatgacg actacctcaa cggcaggctg
     4381 cccgacgagc agggctgagg gcggcggcga cgtgcgggac ggccggatca gcatccgcag
     4441 ggcgcagtca gtggatcggg cgcggcggcg gtcgtgccgg tggccgtctc gacagggaag
     4501 ccctcccgga tccagtactc gatcccgccg atcatctccc tgacccggta gccgaggcga
     4561 gagagggtga gggccgccct ggtggcgccg ttgcaccccg gtccccagca gtaggtgacc
     4621 agcggcatgg cggggtcgag caggtgggga gcacgcccgg gaatctcgtc cgtgggcagg
     4681 tgcagggcgc ccgggatgcg tccctggcgc cacgcggcac cgccgcgcga gtcgacgagc
     4741 acgaagccgg gacggtcgcc ggccagggcg gcccggacgt cggccacatc ggtctggtag
     4801 ctgaggcggg cggtgaagaa cgcgactgcg ccctggtgct cggcgggggt cggagcggga
     4861 gttgggagat cgagggtcac ccgtcggacg gtaggtgcgc agggcccgat gatgaaggag
     4921 gaatccccgg ctgattccgg aaaacgccgt ggcttccacg gagtcgacga gcgggcatgc
     4981 ggcatcatcg gacgaaatgg acggtagatt cgccgggtgt ccgtggaatc cctcgacgac
     5041 gtggactggc gcctgctgga agcgctccaa cgtgatggcc gggcgagcta cgccgacctg
     5101 ggccggctgg tcgggctctc gcccagtgcc gtcaccgagc gcgtgcgcag gctggaggaa
     5161 tccggggtga ttaccggcta caccgccgag gtgaacccgg agaagctggg cctgccgatc
     5221 acggccctgg tccggctgcg ctatccgcac gggaactaca agccgttccg ggacctgctc
     5281 gcggccaccc cggagatcac cgaggcgcac catgtcacgg gcgaggactg cttcgtgctc
     5341 acagtgcgga cccgttcgat gcgccacctc gaggaggtca ccggacgcat ctccggcctc
     5401 ggtgccgtga ccacgagcgt cgtgtactcc agtccgctgc cgcgccgctc ccgtcggcac
     5461 ggcgatgggc gcaggcacta ccgcggggaa gtcctcccgc cgcaggagct gagctcccac
     5521 tccgccgatt cgtaagacga tcgagacgtg gtcagccggg cagggcgcgc agcagcgtgc
     5581 cgggtccgat cgtttccgct ccggtcgaac gcacgcgttc gcgcagctct cggtcggcgg
     5641 tcaccacaag cacctgctcc gaggcgagat cggcggccag cgccgcgatc gccgtgtccc
     5701 cgtcggccgg cgcgcggacg acgtcgagct gggggtgggt gggcagatcc tccacgcggg
     5761 ctgcggcgcc ctccaggacg aggtgcacca tcacgtcctc gtccgaggct tcccggccca
     5821 gggcctcggc gagccgggcc ggcccggcgg tcagcgcggc cgacagcttc ccggcgagcc
     5881 gacgggcagc acccggccgg tcgcgccacc agccgttcgg gcgcgagccc accacgttcg
     5941 ccccgtcgac gatcacgtgg agcatgggca cctccggggt gctcacggga tgacgggcgc
     6001 ggcgccggtc acgcgatcgg tggtaccagg tcccggggga gtttcgacgc cgccgcacgg
     6061 tcgagcagcc acagggtgcg tcgccgcccc cgcgcacccg ccaccgggat cgcgacctcg
     6121 cccgcgccgc cgagcgccat tgcgaccgcc gccgccttcg actcgcccgt cgtcatgatc
     6181 cagacctcgg ctgcgcgacg gatggccggg agagtgagag tgacgcgcgt gggcgggggc
     6241 ttggggcaac cgtgcacggc caccaccgga cgctcggtct cgtacaccgc gggggagtgc
     6301 gggaacaccg aggcggtgtg gccctcctcg cccatgccga gcatcagcac gtcgaacgag
     6361 gggaccggcc cgtgatcctc cgggcgggcc gcccgggcca gcaccgccgc gtactcctcg
     6421 gccgccgcct cggcgccgcc agggccggtg cccgtgtctg cgcccatcgg gaagatcttg
     6481 gccgggtcga ccgggatgtg gtcgagcagc gcctcccgcg cctgcttctc gttgcgctcg
     6541 gggtcgtcgg gcggcacgaa ccgctcgtcg ccccagtaga actcgacgcg actccagtcg
     6601 acggcgtccc gcgccacgct ggcgcggatc tgctccagca ccgcgatgcc cgtgctgccg
     6661 ccggtgagca cgatcttcgg cgtgttgccg gccgcctgca ggtcgaccag ccgtgtcacc
     6721 aggcgtgctg ccgccgccgc ggcgagcaca ttcgggtgcg agtggacggc gatgtccgga
     6781 gtagccatca ggagccggcc gtaagggcgg ggaccttgac cggggtacgc ccgcgcgcga
     6841 tcagtgagac accggccaac gcggcgccgt agatctcgtc ggggtcgagc ctgcgcagct
     6901 cctcggcgag gcagtcgcgc acctcgcgac gtgcgagcgc cacgagccgg tccggctggc
     6961 cgggctgacg caggctcccc accttgccgt ccgggcggat cagctcgatc gggcctgacc
     7021 tgcggtcgag ccgcacggac accagccccg gcccggccag ggcgggcgac cgcttgacct
     7081 tcgcgtcgag ccggaccgcg agccagccgg ccagcaggtc ggtggacggc gacacggcct
     7141 cgcccgcgac ggtggccccg gtgacgtcct cgtgcggtgg cgcgtccaga gcggtggcga
     7201 gcagggcccg ccacgccgtg agccgtgtcc acgtcaggtc ggtgtccccg gccacgtggt
     7261 tcgcccgccg ctgctcgaac gccctgagcg ggttcttgga ggacagggcg tcggtgatcc
     7321 ggcgctgcgc gagccgcccg accgcgtcct gcgacggcac cgcgggcgcc tcacccggcc
     7381 accacgccac caccggcgcg tcggggagca gcagcggcat caccatgccg gcgccggcct
     7441 cgtcggcggc caacgcgccg tagccgcgca gcacgatcac ctcactcgcg ccggcgtcac
     7501 cgcccacgcg gatctgcgcg tcgagacgcg gagcggcctt gcgttgcccg cgggcgagca
     7561 cgatgacgcg gcaggggtgc tcccggctcg cggagttcgc ggcctcgatg gcgccctcga
     7621 tggcgctgcc gtcgtcggtc aggatcacga gcgtgagcac gcggcccagc gccagcacgc
     7681 cgttgctctc gcggagctcg acgagcttct tgttgaccgc cgaggtggtg ctcgacggca
     7741 ggtcgacgat catgtccagc tccctggtgc ggatcgggcg gggttcacgg cctgcgccag
     7801 gtgcggccgg tgcgtgccag catcgcgttg gccgactcgg ggccccagct gccggcgggg
     7861 tacgggtcgg ggccctcctc ctgggcggcc cagtgctcca gcaccgggtc gaggatctcc
     7921 caggagcgct cgacctcctc gttcaccggg aacagtgacg gctcgccgag cagcacgtcg
     7981 aggatcagcc gctcgtaggc ctcgggggac gcctcggtga acgcggcgcc gtagccgaag
     8041 tccatcgtga cgtcgcggac ctccatctgg ctgcccggca ccttcgaacc gaaccgcatc
     8101 gtgacgccct cgtcgggctg caccctgatc acgaacgcgt tctgcccgag ctcctcggtc
     8161 atcgtcgcgt cgaacggcag gtgcggcgct cgcttgaaga tcacggcgat ctcggtgacg
     8221 cggcggccga gccgcttgcc ggtgcgcagg tagaacggca ccacggccca ccggcggctc
     8281 tgcacttcga gcgtcaccgc cgcgtaggtc tcggtcttcg agtccttcgc gaaaccctct
     8341 tcctgcagca ggccgggcac cttcgtgccg ccctgccagc cgccggcgta ctgcccgcgc
     8401 gcggtggtct gctcgaacgg ctccagcggc ttggtcgccg ccagcacctt cgccttctcc
     8461 gcgcgcagcg acttcggctc gaacgacagc ggctcgtcca t
//
LOCUS       DASRSC010000127         8074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4955404,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000127 DASRSC010000000
VERSION     DASRSC010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4955404"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VFE39_05925"
     CDS             <1..717
                     /locus_tag="VFE39_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258173.1"
                     /translation="VGLIAATVALLAARTATSDTTTHASAPAVVSAPQQAPPPPAAGT
                     ASSLPPSQPGAVGSASDPVGLSALSGQQVPPSSTPATSEELFPDDTPRQSVPVPPPVP
                     VPLTGPVPCTNAMITVGAEIDRPEHHVGERPVLRLVVTNISGQPCIRDLDSARQEIVV
                     WSPEGARLWSSNDCVNASSTDLRTLVPGQPVAFAVRWAGRTSTPGCATVRTVVPPGPY
                     RVRTRVDDDISAATPFVRLA"
     gene            complement(730..2097)
                     /gene="radA"
                     /locus_tag="VFE39_05930"
     CDS             complement(730..2097)
                     /gene="radA"
                     /locus_tag="VFE39_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET6258174.1"
                     /translation="MTPRTRTARTGYRCGECGHLAAQWVGRCPSCQAWGSLEQSAPVA
                     AAPARTRVAAGAPTAPARRIADVALDSTRARPTGVSELDRVLGGGLIPGSVVLLAGEP
                     GVGKSTLLLEVAAKAASTGRVLYVTGEESAGQVRLRAERTGAVHDELFLAAESDLGAI
                     VGHIDELRPDLLVVDSVQTMSTGDADGTPGGVTQTRAVTVALTGLAKDRGLPVVLVGH
                     VTKDGAIAGPRVLEHLVDVVLAFEGDKHSTLRMVRGVKNRFGPADEVGCFELRDEGIV
                     GMPDPSGLFLARFGGPPVPGTAVTVVMDGRRPLPAELQALVTGKDIPSPRRAVSGLDN
                     ARVAMLLAVLEKRAGVRLHDAEVYAATVGGMRVVEPAADLALALAITSARRDVALPSD
                     VVVLGEIGLAGEVRRVTGVDRRLAEAARLGFTHALVPPDSGVAPAGMRITEVRDVGVV
                     LDAIR"
     gene            complement(2182..2808)
                     /locus_tag="VFE39_05935"
     CDS             complement(2182..2808)
                     /locus_tag="VFE39_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258175.1"
                     /translation="MRIRPTGAALVGGAMAAALALTGCGAGQLAQTSEQVASVDGANV
                     MVGAISIGDAVIEFRAPQGSSVYPIGGSAPLQMTIVNVGAQADRLVSATSPVATSAQI
                     SGKVEIPAGQALLVQGAPTAGAAPGAGAPAAPGPTTAPAQPTSPAATPSPAAEPGAGE
                     ASIVLTGLRTEIQAGLTYPVVLTFERAGQVSFNVPVSNPAGPRSAPTE"
     gene            3105..3596
                     /locus_tag="VFE39_05940"
     CDS             3105..3596
                     /locus_tag="VFE39_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HET6258176.1"
                     /translation="MAFKVGETVVYPHHGAALIEAIETRTIKGEERKYLVLKVAQGDL
                     TVRVPAENAEVVGVRDVVGQEGLDRVFEVLRAPHTEEPTNWSRRYKANLEKLASGDVN
                     KVAEVVRDLWRREKDRGLSAGEKRMLAKARQILVSELALAEGTDEERAEILLDEVLAT
                     ASA"
     gene            3638..4315
                     /locus_tag="VFE39_05945"
     CDS             3638..4315
                     /locus_tag="VFE39_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET6258177.1"
                     /translation="MSITAVVVAGGRGEVDVLASVSGEPMVARSVRVLLATGLVDHVL
                     LLDVGMRGDSLIRACSGLPVSMRDEFPRTLSGMRPHGTQRADTTAGDGVVTGSSTDVV
                     LLHDAARPLAPPELALAVVAAVRRGHELAVPVLPLADTVKQVDAEGIVRSTPDRSGLR
                     VVQTPIAIRGDLVDPGLAADPVAMATRHAAAGGAVHTVPGHSAAFTVRNAWDLELAEL
                     LAGKLAR"
     gene            4312..4779
                     /gene="ispF"
                     /locus_tag="VFE39_05950"
     CDS             4312..4779
                     /gene="ispF"
                     /locus_tag="VFE39_05950"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HET6258178.1"
                     /translation="MRVGLGTDVHPVEAGRDCWIAGLRWDGVDGCAGHSDGDVAAHAL
                     CDALLSAAGLGDLGAVFGTGRPEWTGASGVALLGEVRRLLSDEGWTVGNAAVQVIGNA
                     PKVGTRRAEAQQVLSAAVGGPVSVSGTTTDGLGLTGRGEGIAAIATALLLPAP"
     gene            complement(4819..5250)
                     /locus_tag="VFE39_05955"
     CDS             complement(4819..5250)
                     /locus_tag="VFE39_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6258179.1"
                     /translation="MTEILGLHPRLVVEGADAALAFYTTAFGGEVLERYTGSDGRVVH
                     SMVQVGPARFAVKDAGDGDAAPTGGGVPVIMSLSVSDADVVAERMLAAGATVIFEIAD
                     HGYGDYGGRLADPFGHQWMLSERIEQLDADQVQERLDAMQA"
     gene            5345..6736
                     /gene="cysS"
                     /locus_tag="VFE39_05960"
     CDS             5345..6736
                     /gene="cysS"
                     /locus_tag="VFE39_05960"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET6258180.1"
                     /translation="MSLRLFDTATRKQRDFVPLRAGAASIYVCGATVQGLPHIGHVRS
                     GLNYDVLRRWLAHGGLDVELVRNVTDIDDKILRKAADAGRPWWEWAATHERAFSAAYD
                     ALGCLPASIEPRATGHVPQMVELMDRLIEGGHAYGAGGDVYFSVRSFPGYGALSHQKV
                     DDVAQGEGESGAKRDPVDFTLWKAAKPGEPSWPTPWGRGRPGWHLECSAMATAYLGSE
                     FDIHGGGLDLVFPHHENERAQSNAAGDPFARYWLHNAWVTMGGEKMSKSLGNTLSIDV
                     LLRRVRGVELRYYLVGPHYRSAIEYSDAALHESVAAYRRIESFVHRVRERIGTPEPGV
                     LCAEFTAAMDDDLGTPGALAAIHGTVREGNTALDAGDRSRAAGAASSVRAMAGILGLD
                     PLDPHWIEGGGAADAASGALTALVEDLLAQRQEARARRDFAAADEVRDRLTAAGVSVE
                     DSPDGPTWSLKDV"
     gene            6742..7716
                     /gene="rlmB"
                     /locus_tag="VFE39_05965"
     CDS             6742..7716
                     /gene="rlmB"
                     /locus_tag="VFE39_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET6258181.1"
                     /translation="MAGNSKRRGAMRKDGTKKGMVVGSGGQRRRGLQGKGPTPPAEMR
                     PGHPAARKASRGSKAGSGGRSTTAGNRRRPGDGETPETVLGRNPVVECLRAGVPATAL
                     HVAIGTTTDERVAEAVHLAADAGISILEVPRNDLDRIAGGALHQGLALQVPPYAYAHP
                     DELLEIAAESGDPGLIVALDGVTDPRNLGAVVRSVGAFGGHGVVVPQRRAAGMTAVAW
                     RTSAGTAARLPVARATNLTRTLREYATAGFMIAGLDAEGSVSLDDFELATDPLVLVVG
                     SEGKGLSRLVKQTCDVTVSIPMAGPVESLNASVAAGVVLAEIARQRRH"
     gene            complement(7775..>8074)
                     /locus_tag="VFE39_05970"
     CDS             complement(7775..>8074)
                     /locus_tag="VFE39_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET6258182.1"
                     /translation="LVFAVLIGLTTALAVPIVGAILVLSVMIAPGASAARVTANPVVA
                     TVLAVLFAELALVGGTVLSLAPGLPVSGYVAGIAFLCYVGCRVVGRLRGRTRAAA"
BASE COUNT         1127 a         2852 c         2950 g         1145 t
ORIGIN      
        1 gtcgggctga tcgccgcgac cgtggccctt ctcgcggcgc gcaccgcgac gtccgacacc
       61 accacccacg catccgctcc tgcagttgtg tccgctcccc aacaggcccc accgcccccg
      121 gcggccggta cggcatcctc cctgccgccg tctcagcccg gggcggtggg gtctgcgtcc
      181 gacccagtcg gcctgtcggc tctgtcgggg cagcaggtgc ccccgtcgag cacccccgcc
      241 acctccgagg agctgttccc cgacgacacg ccgcgccagt ccgtgccggt ccccccgccg
      301 gtgcccgtac cgctgaccgg tccggtgccc tgcacgaacg cgatgatcac cgtcggggcc
      361 gagatcgacc gtcccgagca tcacgtgggg gagcggccgg tgctgcggct ggtggtcacc
      421 aacatcagcg gccagccgtg cattcgcgat ctcgactcgg cccggcagga aatcgtcgtg
      481 tggagccccg agggggcacg gctctggtcg agcaacgact gtgtcaatgc ctccagcact
      541 gacctccgca ccctcgtgcc agggcagccc gtcgcgttcg cggtgcggtg ggcgggccgc
      601 acctcgacgc cgggctgtgc gaccgttcgc acggtggtgc ctccgggccc ctaccgcgtg
      661 aggaccaggg tcgacgacga catcagtgcg gcgaccccgt tcgtccggct cgcctgacgc
      721 gcctgcccct cagcggatcg cgtccagtac cacgccgacg tcgcggacct cggtgatcct
      781 catgcccgcc ggggcgacgc cgctgtccgg ggggacgagc gcgtgggtga agccgagccg
      841 ggccgcttcg gcgagccttc ggtcgacacc ggtgactcgg cgcacctcgc cggccagccc
      901 gatctcgccg agcaccacga cgtccgacgg cagggccacg tcacggcggg ccgaggtgat
      961 ggccagcgcc agcgcgaggt cggcggcggg ttcgaccacc cgcatgccgc ccacggtggc
     1021 ggcgtagacc tccgcgtcgt gcagccgtac gcccgcccgc ttctcgagca cggcgagcag
     1081 catcgcgacg cgggcgttgt cgaggccgct gacggcacgc cgcggactcg gtatgtcctt
     1141 cccggtgaca agcgcctgga gttcggcggg gagcggccgg cggccgtcca tcaccaccgt
     1201 gacagcggtg ccgggcacag gcgggccgcc gaaccgcgcc aggaagagac cggacgggtc
     1261 gggcatcccg acgatgccct cgtcgcgcag ttcgaagcac cccacctcgt cggccgggcc
     1321 gaagcggttc ttgacaccgc gcaccatgcg aagcgtcgaa tgcttgtccc cttcgaacgc
     1381 cagcacgacg tcgacgaggt gctcgagcac acgggggccc gcgatcgcgc cgtccttcgt
     1441 cacgtgtccc acgagcacca ccggcagccc gcggtccttg gcgaggccgg tgagtgcgac
     1501 ggtcacggcg cgcgtctgcg tcaccccgcc cggggtgccg tccgcatcgc cggtggacat
     1561 cgtctgcacg gagtcgacga cgagcaggtc ggggcgcagt tcgtcgatgt ggccgacgat
     1621 cgcgccgaga tcggactcgg cggcgaggaa gagctcgtcg tgcacggctc ctgtgcgctc
     1681 ggcccgcagc cgcacctgcc cggcggactc ctcgcctgtg acgtacagca cgcgtcctgt
     1741 gctggcggcc ttggctgcca cctccagcag gagcgtcgac ttccccacgc ccggctcgcc
     1801 ggccaggagg acgaccgagc cggggatcag gccgccaccg aggacccggt ccagttctga
     1861 cacgccagtg gggcgggcac gggtggagtc gagcgcgaca tccgctatcc ggcgggcggg
     1921 agcggtcgga gctcctgcgg cgaccctcgt cctggcgggc gccgctgcca ccggagccga
     1981 ctgctcgagg ctcccccagg cctggcagga cggacaccgc ccgacccatt gggcggccag
     2041 gtgcccacac tcgccgcagc ggtagccggt gcgggccgtg cgggtgcgcg gggtcacggg
     2101 gcttccgcgc cggagatcgc tgtgccagtc accgctgtgc cggtcaccgc tgtgccggag
     2161 gtgcggccgt cgctggttcc gtcactcggt gggcgcgctg cgaggaccgg ccgggttgct
     2221 gaccgggacg ttgaaactga cctgaccggc ccgctcgaac gtgaggacca cggggtaggt
     2281 caacccggcc tggatctcgg tgcgcaggcc ggtgagcacg atgctcgcct cgcccgcgcc
     2341 gggttcagcg gcggggctcg gggtggctgc aggcgacgtc ggctgcgcgg gagccgttgt
     2401 cggccctggg gcggcgggcg ctccggcgcc gggagcagcg cccgccgtgg gcgcgccctg
     2461 gaccagcagc gcctggccgg cggggatctc gacctttccg ctgatctgcg cggacgtcgc
     2521 aacggggctc gtggccgaga ccagccggtc ggcctgggcg ccgacgttga cgatggtcat
     2581 ctgcagggga gcgctgccgc cgatcgggta gacgctgctg ccctgagggg cgcgaaactc
     2641 gatcaccgcg tcgccgatcg agatcgcgcc gaccatgacg ttggcaccgt ccacagacgc
     2701 gacctgttcg ctggtttgag ccaactgccc ggccccgcaa ccggtgagtg cgagggccgc
     2761 ggccatggcg ccgccaacga gggctgctcc ggtcggacgg atacgcatgc tcgggctcac
     2821 gggtctgacg tccttcctgg cgggccatct catcgtgccg cgggcgcagc ggttcgacgg
     2881 acgcggatgc ctggtgtccg cgatggccac tagcttcggc ctcggtaaag cgtaacgcca
     2941 ccggcgggag tggcggtcgg ggtcccctgt cgccgcgggc acagccgccg gtgcacgttt
     3001 cccttcggtt gtcaatcccc tgacctgcga cgacaggtcg ggtcgggcag cggtgccagg
     3061 ttcaggcgtg ttaacctgga tgcagcgaaa ggggcagagg acacatggct ttcaaggtcg
     3121 gagagaccgt cgtctacccg caccacggtg ccgctctcat cgaggccatc gagactcgga
     3181 cgatcaaggg cgaggagcgc aagtacctcg tcctcaaggt cgcgcagggt gatctgacgg
     3241 tgcgtgttcc cgccgagaac gccgaggtag tgggcgttcg tgatgtggtg ggccaggaag
     3301 gtctggacag ggtgttcgag gtgctccgcg ccccgcacac cgaagaacct acgaactggt
     3361 cgcgccggta caaggcaaac ctggagaagc tggcttcggg ggacgtgaac aaggtcgccg
     3421 aggtcgttcg cgacctgtgg cggcgagaga aggaccgcgg cctgtccgcg ggcgagaagc
     3481 gcatgttggc caaggctcgc cagatcctcg tcagcgaact cgcgctggcc gagggcaccg
     3541 acgaggagcg ggccgagatc ctgctcgacg aggtgctggc caccgcgtcc gcctgagcac
     3601 gagcgaactg cccccctccg ccggtcccgg tccccgcatg agtatcaccg cggtggtcgt
     3661 ggccggcggt cgtggtgagg ttgacgttct cgcgtccgtc agcggcgagc cgatggtggc
     3721 gcggtccgtg cgggtcctgc tggccaccgg tctcgtcgat catgtcttgc tgctcgatgt
     3781 gggcatgcgt ggcgactcgc tcatccgcgc ctgctcgggg ctgcccgtga gcatgcgcga
     3841 cgagttccca cgcacactgt ccgggatgcg gccacacggc actcaacgag cagataccac
     3901 cgcgggtgac ggtgtggtca cggggagttc cactgatgtg gttctgctgc acgacgcggc
     3961 ccgcccgctc gcgccgcccg agctggccct ggccgtcgtc gctgctgtcc gccgtggcca
     4021 cgagctcgcc gttcccgtgc tgccgctggc cgacaccgtc aagcaggtgg acgccgaggg
     4081 aatcgtgcgc agcacgcccg accgctccgg gttacgggtg gtccagactc ccatcgcgat
     4141 ccggggggac ctcgtggatc ccggcctcgc cgccgatccc gtcgccatgg cgacccggca
     4201 cgccgcggct ggcggcgcgg tgcacaccgt tcccggccat tcggcggcgt tcaccgtccg
     4261 caacgcatgg gacctggagc tggctgagct gctggcgggg aaactcgcgc gatgagagtg
     4321 ggtctgggca ccgacgtgca tccggtcgag gccgggcgcg actgctggat cgccgggctg
     4381 cgctgggacg gggtggacgg ctgtgccggg cactcggacg gagacgtcgc cgcacatgcc
     4441 ctctgcgacg cgctgttgtc ggccgcaggc ctgggcgacc tcggcgcggt gttcgggacc
     4501 ggccgtccgg agtggacggg cgcgagcggt gtcgcgctgc tcggagaggt ccgccggctg
     4561 ctgtccgatg aggggtggac tgtgggcaac gccgcggtgc aggtgatcgg caatgccccg
     4621 aaggtcggca ccaggcgggc cgaggctcag caagtgctct cggctgcggt cggtggtccg
     4681 gtgtcggtgt cggggacgac cacggatggc ttgggcctga ccggccgggg ggagggcatc
     4741 gcggcgatcg cgacggccct gctcctcccg gccccctgac cgcgatcgtc acccggccgg
     4801 aaccggcgcg tgggcctgtc atgcctgcat ggcgtccagg cgctcctgca cctgatcagc
     4861 gtcgagctgt tcgatgcgct cgctgagcat ccactggtgg ccgaaggggt cggcgagcct
     4921 gccaccatag tcgccgtacc cgtggtcggc gatctcgaag atcaccgtgg cgcccgcggc
     4981 cagcatccgc tccgccacca cgtcggcgtc cgacacggac agcgacatga tcacggggac
     5041 gcctccaccc gtcggagcgg catcgccgtc acctgcgtcc ttcaccgcga accgggcagg
     5101 cccgacctgc accatgctgt gcacgacacg gccgtcggag ccggtgtagc gctcgagcac
     5161 ctccccgccg aacgcggtcg tgtagaaggc gagcgcggcg tcggcgccct ccacgacaag
     5221 ccgggggtgc aatccgagaa tctcagtcat ggcgcgagga tgccgcggtg tgggcgaact
     5281 gtcgtggacg aaccggaact gccctggtac acggtgcggg tgaggggtcc gtactcttga
     5341 agatgtgagc ctgcgcctgt tcgacaccgc cacgaggaag cagcgtgact tcgtcccgct
     5401 gcgggccggg gctgcgtcga tctatgtctg tggcgccacg gtgcagggcc tgccgcacat
     5461 cggccacgtc cggtccgggc tcaactacga cgtgcttcgc aggtggctcg cccacggcgg
     5521 cctcgacgtc gagctcgtgc gcaacgtcac cgacatcgac gacaagatcc tgcgaaaggc
     5581 cgccgacgct ggtaggccgt ggtgggagtg ggcggccacg cacgagcgcg ccttctccgc
     5641 ggcctacgac gcgctcggct gcctcccggc gtccatcgag ccacgggcca cgggtcacgt
     5701 gccacagatg gtcgagctga tggaccggct gatcgagggc ggtcacgcct acggggcagg
     5761 cggtgacgtc tatttctcgg tgcggtcctt cccggggtac ggggcgctgt cgcaccagaa
     5821 ggtcgatgac gtcgcgcagg gcgagggcga aagcggggcc aagcgcgacc cggtcgactt
     5881 caccctgtgg aaggcggcca agcccggcga gccctcgtgg cccacgccat ggggacgtgg
     5941 ccggcccggc tggcacctgg agtgctcggc gatggccacg gcctacctcg gttccgagtt
     6001 cgacatccac gggggcgggc tcgacctggt gttcccgcac cacgagaacg agcgggctca
     6061 gtcgaacgct gcgggcgacc cctttgcgcg gtactggctg cacaacgcct gggtcactat
     6121 gggcggcgag aagatgtcga agtcgctcgg caacacgctg tcgatcgacg tgctgctgcg
     6181 ccgggtccgt ggcgtcgagt tgcgctacta cctggtgggt ccgcactacc gctcagccat
     6241 cgagtactcc gacgccgcac tgcacgagtc ggtggccgcc taccggcgca tcgagtcgtt
     6301 cgtgcaccgg gtgcgtgagc ggatcggcac tccggagccg ggcgtgctgt gcgcagagtt
     6361 caccgcggcg atggacgacg acctggggac gcccggcgca ctcgccgcga tccatggcac
     6421 cgtgcgcgag ggcaacaccg ctctggacgc aggcgaccgg tcgcgcgcag cgggcgcggc
     6481 gtcgtccgtg cgcgcgatgg ccgggatcct cgggctcgac ccgctcgatc cgcactggat
     6541 cgagggcggc ggcgcggcgg acgccgcctc gggcgcgctc accgcactgg tggaggattt
     6601 gctggcccag cgccaggagg cccgcgcccg gcgtgacttc gccgccgccg acgaggtgcg
     6661 cgaccggctc accgccgccg gggtgtccgt ggaggacagt ccggacggcc ccacctggtc
     6721 actgaaggac gtgtaaggac attggccggc aattccaagc gccgcggcgc gatgcgcaag
     6781 gacggcacca agaagggcat ggtcgtcgga tccggggggc aacgacgccg cggtctgcag
     6841 ggcaagggcc ccacgccacc cgccgagatg cgccccggtc acccggcggc gcgcaaggcg
     6901 tcacgagggt cgaaggcggg cagtggtggg cgttccacca ccgccggcaa ccgccgccgg
     6961 cccggtgacg gcgaaacgcc cgagacggtg ctcggccgca accccgtggt ggagtgcctg
     7021 cgtgcaggcg ttccggccac cgcgctgcac gtcgcgatcg gcaccaccac cgacgagcgg
     7081 gtcgccgaag cggtgcacct ggccgccgac gcgggaatct cgatcctcga ggtaccgcgg
     7141 aacgacctgg accggatcgc cggtggtgcg ttgcaccagg ggctcgccct acaggtaccg
     7201 ccctacgcct acgcacaccc cgacgaactg ctcgagatcg cggccgagtc gggtgaccct
     7261 gggctgatcg tcgcgctcga cggcgtgacc gaccctcgga acctgggcgc ggtcgtccgg
     7321 tcggtgggcg cgttcggcgg ccacggcgtc gtcgtgccgc agcgccgggc ggcaggtatg
     7381 acggccgtgg cgtggcgcac ctcggcgggc acggcggcac ggctcccggt ggcccgggcc
     7441 accaacctca cccgcaccct gcgtgagtac gccaccgcgg gcttcatgat cgccgggctc
     7501 gacgcggagg gttcggtgtc cctcgacgac ttcgagctgg ccaccgaccc gctggtgctc
     7561 gtggtcggct cggagggcaa gggcctctcg cggctcgtga agcagacgtg cgatgtcacc
     7621 gtgtcgatcc cgatggcggg gcccgtcgag tccttgaacg cctctgtggc ggcgggcgtc
     7681 gtcctggccg agatcgctcg tcagcgtcgc cactgacccc cgtgcgcggt cccgagtgtc
     7741 agagcactca taaccgcgca cgaccgctgg agggtcacgc ggcagcgcgg gtgcgtccgc
     7801 gcagccggcc cacgacgcgg cagccgacgt agcagaggaa tgcgatcccg gcgacgtagc
     7861 cggacaccgg cagccccgga gcgagtgaca gcaccgtgcc cccgacgagg gcgagctcgg
     7921 cgaacagcac cgcgagcacg gtggccacca ccgggttcgc cgtcacgcgc gcagccgacg
     7981 cccccggcgc gatcatcacc gacagcacga ggatcgcgcc gacgatcggc acggcgaggg
     8041 ctgtggtcag cccgatcagc accgcgaaca ccag
//
LOCUS       DASRSC010000128        21289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_371744,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000128 DASRSC010000000
VERSION     DASRSC010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 21289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21289
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371744"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFE39_05975"
     CDS             complement(<1..279)
                     /locus_tag="VFE39_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HET6258183.1"
                     /translation="MGTALIYLMIVAAVAAVFFGLAAVVFGRGEELAPLPPGATPTRL
                     PAGELDGEDVRELRFQQALRGYRMDEVDWVLDRLADELDRAGVERDGLL"
     gene            complement(307..1236)
                     /locus_tag="VFE39_05980"
     CDS             complement(307..1236)
                     /locus_tag="VFE39_05980"
                     /EC_number="2.4.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyl-3-phosphoglycerate synthase"
                     /protein_id="HET6258184.1"
                     /translation="MERVTEEWFGPRTWQEPGWSIDELVAAKAHRRVSVVLPALDEEA
                     TVGAIVAAIVPLTLGTVPLVDELVVMDSGSTDRTVELAAAAGATVVLRTDVLTELPPV
                     PGKGEVLWRSLAATTGDVICYLDSDLVDFDAGFVPALLGPLLTEPGVALVKGFYRRPL
                     RLETTEADTGGGRVTELLVRPLLAALRPELAGIVQPLGGEYAGTRELLESVPFAAGYG
                     VEIGLLLDTYATLGLDALAQVNLGVRKHRNRSLLQLGVMARQILAAALSRCGIPDTGA
                     ALTQFLQVDGEWLPSSTAIGGTERPPMREVLVR"
     gene            complement(1288..2103)
                     /gene="folP"
                     /locus_tag="VFE39_05985"
     CDS             complement(1288..2103)
                     /gene="folP"
                     /locus_tag="VFE39_05985"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020634280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET6258185.1"
                     /translation="MAIVNRTPDSFYDRGATFADGAAMAKVDAALAEGADIIDIGGVK
                     AGPGPDVDVAEEVRRVVPFVAAVRERYPDVVISVDTWRGEVGRLAVAEGADLLNDTWA
                     GADPALGDVAAETGVGIVCSHTGGALPRSRPHRVRYADVVADVLADVCGQAERLVALG
                     VRREGILVDPTHDFGKNTFHGLTLLRRCDELVAAGWPVLMALSNKDFVGETLGVAEVT
                     ERLEGSLAATALAAAAGARVFRAHEVAATRHTVDMVAAIAGTHPPARTVRALA"
     gene            complement(2166..2741)
                     /locus_tag="VFE39_05990"
     CDS             complement(2166..2741)
                     /locus_tag="VFE39_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET6258186.1"
                     /translation="MTGRSVAVCVYCASIEGIAPHYLELAAEVGRQIATRGWELVSGG
                     GRAAMMGAIGAAARAGGARTTGVIPRALVDLEWADHDSDELLLVDTMRERKALMEAHA
                     DAFLALPGGIGTCEELFEVWTAGYLRMHAKPTVLLDPDGHYTGLLDWVRGLGERGFVR
                     PEALARLVVVTDVAAALDACASPVDMSPSRT"
     gene            complement(2738..3517)
                     /locus_tag="VFE39_05995"
     CDS             complement(2738..3517)
                     /locus_tag="VFE39_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HET6258187.1"
                     /translation="MTASGNRRPDDKQPEQKQRGPVVLRREYRNESSTTDQRLLDSRG
                     PSDWVHTDPWRVLRIQAEFVEGFGTLAELPRAVTVFGSARTGPDHPEYAQGLALGTAL
                     AEAGFAVITGGGPGVMEAANRGCSEAGGLSVGLGIELPFEQGLNNWVDLGINFRYFFA
                     RKTMFVKYSQAFVCLPGGFGTLDELFEALTLVQTKKVTKFPVVLLGSEYWGGLYEWIA
                     NTVHASGKVGDKDLALLHVTDDVDDAVKIVQEAWRAWEEAH"
     gene            complement(3610..4674)
                     /gene="dapE"
                     /locus_tag="VFE39_06000"
     CDS             complement(3610..4674)
                     /gene="dapE"
                     /locus_tag="VFE39_06000"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HET6258188.1"
                     /translation="MPVLDLTSDPVELCAALVDVPSVSGDEATLADAVELALRKQASH
                     LELLRCGDAVLARTNLGRERRVLLAGHLDTVPIADNVPSRRDGDLLYGCGTTDMKSGD
                     AVLAHLAATLTEPRHDLTLVFYDCEEVEADRNGLNRIQREHRDWLHADLAILAEPTNA
                     GVEAGCQGTLRAEITTRGRRAHSARSWHGRNAVHAAAAVLERLNTYTAREVDIDGCVF
                     REGMQAVRIAGGVAMNVVPDECVVTVNFRFAPDRDSAAAEAHVRDVFDGFELAVTDLA
                     PGALPGLTAPAAGEFIAATGATPSAKYGWTDVSRFAALGIPAVNYGPGDPNVAHTREE
                     HVHTRQITDTATVLRRFLSP"
     gene            4776..5726
                     /gene="dapD"
                     /locus_tag="VFE39_06005"
     CDS             4776..5726
                     /gene="dapD"
                     /locus_tag="VFE39_06005"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HET6258189.1"
                     /translation="MSTAPRREGASGTGLATVTVPVDGSGGVVLDTWFPAPALGGQAP
                     DGAADLEHLIGTDEARGVRTVVVRTAIDSLADPPADPYDVYLRLHLLSHRLVAPREVN
                     LDGIFGLLANVVWTDHGPCAAPGFELTRARLRARGPVTVLGVDKFPRMVDYVLPTGVR
                     IADADRVRLGAHLAEGTTVMHEGFVNYNAGTLGASMVEGRISAGVVVGDGSDVGGGAS
                     IMGTLSGGGKEVVSVGKRCLVGANAGIGISLGDDCVVEAGLYVTAGTKVVLPSGETAA
                     ARTLSGQNGMLLRRNSVTGAVEVLARTGDGIALNAALHTN"
     gene            5756..6739
                     /locus_tag="VFE39_06010"
     CDS             5756..6739
                     /locus_tag="VFE39_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HET6258190.1"
                     /translation="MPTLASRRLQQLPFDVADLPAPLSVTPSDPPAQHVRAALDLRLR
                     ALLYHDPGTRSGADIEDLHQMRVAVRRMRAALKAARPLLDSAWADGLRAELGWLGRAL
                     GPVRDLDVMLLRLRREVASLPERERAAGGVLVDSMEGEHAAARALMLATLDAPRYTAL
                     LERLADAIRLPLPTPSATRAQPELVDLVRAEAHRMRRAVEKAGADPPDAVLHELRIFG
                     KRLRYTVELVEPGLRGQALQGRSEAGQGRAVKRLLAATAQLQEVLGDHQDACVAEQRI
                     RELLDGLGELPDAHVVFVAGRLVERERARAEHKRAEWPSAWTQVAERIADF"
     gene            complement(6764..7969)
                     /locus_tag="VFE39_06015"
     CDS             complement(6764..7969)
                     /locus_tag="VFE39_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6258191.1"
                     /translation="MTSGSGTTSSRRAGTRGVRPGVVLLGLLVLAVNLRAVLAGYPPL
                     LETVRAELGTSAAVAGFVQAGAVLMMAVGSFAGSAVGARFGRERALGGSVGLVALGSM
                     VRGVPALLPLIGGSLLIGLGIGLAGVLLAGVVKEHLAERAGTATGGYVVSMMVGATVA
                     SAVAVPLAAALGGWSRSLAVWAVPAVLAVAIWVPVARRTGAPDRAAPRLSLPWRSGFA
                     RTVACYQAGTSLMFYGWLTWLAPYYEGQGWSSHRAGLLLAVWSVAQIPAALLVPALAE
                     RRRRWRFWSGLTLGAGAAGTLGAMVLPTLPVAGPWLWAVLIGIGVGAGFPLGLTVIAW
                     RTADGAASAATSGLALGVGYLSAGLGPLVMGALIDLTKGFSAALAVLLAAAALQGWAA
                     WRIGDRPAH"
     gene            complement(7966..9060)
                     /gene="dapC"
                     /locus_tag="VFE39_06020"
     CDS             complement(7966..9060)
                     /gene="dapC"
                     /locus_tag="VFE39_06020"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HET6258192.1"
                     /translation="MKLDLPDFPWDSLAEDKALAAAHPGGIVDLSVGTPVDPVPDVVR
                     AALAGPAAHEPGYPTTHGPDSLREAIAGAMARRFGVTVDPVAVLPTIGSKELVAWLPT
                     LLGLGPGDTVVIPELAYPTYEVGARLAGTEFVRTDSTAALGPQRTALTWLNSPSNPTG
                     RVLPPEHLRKVVDWARERGAVVASDECYLSLAPASQRAVSVLHPDVCGGSFENLLAVH
                     SMSKTSNMAGYRGGFVTGDPSLVADLLEVRKHAGMMVPRPVQAAMTAAASDDTHVVAQ
                     AEVYTRRRARLRVTLEAAGLRVDHSEAGLYLWATAGEPSRLTVRRLATQGVLVAPGEF
                     YGPAGAQHVRVALTATDERIAAAAERLLEA"
     gene            complement(9091..9417)
                     /gene="fdxA"
                     /locus_tag="VFE39_06025"
     CDS             complement(9091..9417)
                     /gene="fdxA"
                     /locus_tag="VFE39_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET6258193.1"
                     /translation="MTYVIAEPCVDLLDKACIEECPVDCIYEGGRMLYIHPDECVDCG
                     ACEPVCPVEAIYYEDDVPEQWSAYTKANVDFFDELGSPGGASKIGKIDRDVEPAASLP
                     PQQHDE"
     gene            9542..10498
                     /locus_tag="VFE39_06030"
     CDS             9542..10498
                     /locus_tag="VFE39_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6258194.1"
                     /translation="MEISDELVGARVALRYRIGQRDGRPLYSDAVGELSAAGPDAVLV
                     QTRRGPVHVDRAAVVAVRAVPPAPARRPSLTAITRLEGLSADAWPALVDRPLGAWRLR
                     AAGGYTGRANAGLVLGDPGMPIPAALDAVRAFAHEHEIPARVHVPIGSPWDRAVAAQG
                     WVLDVGHAAGAEVAVLVGRLAGIRDPRVGLPERPSDAWWQLALGGPPTPAQRHVLDPG
                     GHPPTAFAEIPGPDGAATAQVRATVVEDHLHVSKLEVIPAARRQGLATALLASVAEWG
                     REYGARWAVLQVPLQNSGARALYGRLGYVEHHQYRYLVPPPG"
     gene            complement(10605..10994)
                     /locus_tag="VFE39_06035"
     CDS             complement(10605..10994)
                     /locus_tag="VFE39_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258195.1"
                     /translation="MGEPEPPAVSDGGLFGLLILDGVLLGGLGLVLTPLYAGGVPVPI
                     GVVLSVLILPWLVRRAGEVDARPSRAAAPLSAWVLTVAVLGLVGPGGDIMLPATWQSL
                     LLVVGGVGSGLWALRGVLNTEHQRTGG"
     gene            complement(10987..11862)
                     /gene="mshB"
                     /locus_tag="VFE39_06040"
     CDS             complement(10987..11862)
                     /gene="mshB"
                     /locus_tag="VFE39_06040"
                     /EC_number="3.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-
                     D-glucopyranoside deacetylase"
                     /protein_id="HET6258196.1"
                     /translation="MTPAPRRRLLLVHAHPDDETLATGGTVARYAAEQDTSVTLVTCT
                     LGEQGEVIPQDLALLAPGKADQLGGYRIGELARACTALGVRDHRFLGGAGRWRDSGMA
                     LAGHGVRAATPEHLHPRAFARPEAFDEQVDTLVAVLEEVRPQVVVTYAADGGYGHPDH
                     VRAHEITVAALRRVPARLYFTVIGRGALDAGRAALAAVPGLPFRLPGPDELPSVPDAA
                     ITVRVDVTAQRAARIAAMRAHATQIALWESGDAIALAMSNLVAQPLLDVEEYVAADGA
                     GDPLAADLFAGGDHG"
     gene            12113..12711
                     /locus_tag="VFE39_06045"
                     /pseudo
     CDS             12113..12711
                     /locus_tag="VFE39_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496487.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(12721..13761)
                     /locus_tag="VFE39_06050"
     CDS             complement(12721..13761)
                     /locus_tag="VFE39_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6258197.1"
                     /translation="MHESGSAATDPEPILSARNLVKHFPIRSTGLVRRQVGVVHAVCD
                     VSFDLTPGETLGLVGESGCGKSTTARLVLNLIEATSGQVFYNGTDLTTLPSKKMRELR
                     RDLQIVFQDPYASLDPRMPVNEIVAEPLRIHGRYDDAGKTQVRDLLRTVGLKPEHGNR
                     FPHEFSGGQRQRIGVARALALRPKVLVLDEPVSALDVSIQAGVLNLLDELQSELGLSY
                     LFVSHDLSVVRHTADRIAVMYLGRIVETGTASELFEGPAHPYTQALISAIPLPDPRKQ
                     RARERITVVGDVPSPADPPSGCRFRTRCPKFAAELSDAERGRCVDVVPELVDRGQGHP
                     AACHYAERKVLI"
     gene            complement(13862..14941)
                     /locus_tag="VFE39_06055"
     CDS             complement(13862..14941)
                     /locus_tag="VFE39_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6258198.1"
                     /translation="MPRNGAASDVVLEVSDLTVGFPSDDGLVQAVRGVSYGLRRGEVL
                     GIVGESGSGKSVTSLAVMGLLPGNAKVSGSVRFGGKELLRAPEAELNKVRGRNVAMIF
                     QDPMTSLNPVYSIGFQVAEAVRAHHNVSKAAALDRAAELLELVRIPNPRQRLENYPHE
                     MSGGMRQRVVIAIAMANNPDVIIADEPTTALDVTVQAQIMESLQLAQQETGAAMVLIT
                     HDLGVIAGQADRVMVMYAGKLVEVGTAEDVFYTPRMPYTFGLLGSLPRLDRPSERLTP
                     IQGAPPSVVNMPPGCPFTPRCPLSVAVCEEVEPALAPTTGPEHLAACHRSDALVGATP
                     GDVFETDVVDTEALAHFVRKTEGQA"
     gene            complement(14977..15918)
                     /locus_tag="VFE39_06060"
     CDS             complement(14977..15918)
                     /locus_tag="VFE39_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6258199.1"
                     /translation="MREVAPTPETSPLPAPGTGRVPAAEREFTVGERSQAQLVLRRFL
                     QHRAAMISLVVLVLVVLLAYVGGALWKYHAGEITPDNSQPPSFDHPFGTDAVGKDQFA
                     QVLRGTRISLQVAVLVAVFATVVGTVWGAVAGYFGGRADSTLMRLADLVLTLPLIAVA
                     AMLAHNFGGTWYLIAMVIAVLYAPYVSRVSRGVVLSLREKEYIEAAKALGATDARIIL
                     RHLVPNALGSILVNLTILVALAILLETALSYLGFGVQTPDTSLGLLVSEAQTALTTRP
                     WLFYFPGLFIIVIALTINFIGDGLRDAFDPQQRKVRQ"
     gene            complement(15911..16891)
                     /locus_tag="VFE39_06065"
     CDS             complement(15911..16891)
                     /locus_tag="VFE39_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6258200.1"
                     /translation="MLAFAVRRIAISIPILLLASFIVFALGVSSGNPLAPLLAKNPPA
                     PPNVIAAETVRLHLDQPLIPRYFSWLAGLLHGDFGPSVVSTVNIGSELGARFWVTIRL
                     IALAMVIALVLAVVVGVLSAVKQYTVTDYTTTFLGFLFLAMPSFWLAILLKQGGIEFN
                     KAVDAQVVYTIGATSIPPPPGTGAQIVDVLGHMVLPTIALALISYASWSRFTRASMLE
                     VMNSDYVRLARSKGLSRSTVMVKHALRTALIPLTTVTALDIASIIGGAVVTETVFQWK
                     GMGDFLLNAIRTEDVYAASSWLLLTGVIVVVFNLIADLLYGVLDPRIRYA"
     gene            complement(17069..18817)
                     /locus_tag="VFE39_06070"
     CDS             complement(17069..18817)
                     /locus_tag="VFE39_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HET6258201.1"
                     /translation="MTRTRAASLVAMSAAAALVLAACGGGGGGGGGGGSSTSTGLFQN
                     CADANTCNAVPADQLQQGGQISFAIEKNIDNWNLNSSEGNVFETSMALKPLLPYAFIT
                     TPDLKSTMNPDYVTSAEVTSTSPFVITYKINPQAKWDDGTPVTADDFVFNWKTQNGRD
                     CPDCETAGNGGYDQITSVVGSDGGKTVTVNWSKPYTDWKSIFNSSAPLYPAHIAAQHG
                     DLNTPAGLKSAFDYLGSTVINYSAGPFKVDNWQPNVALTMVPNPAWWGTKPKLDRLVF
                     RVITDATQEPLALQNNEVQVIYPQPQVDLVSQVAQIPNVSQTQGLGLTWEHFDFNLVN
                     PFLADKALRQAMYTAVNTQDIIAKTVGQFNHDVKPLNNHMFIPQQDGYTDNITPTGQG
                     SGNIDAAKKILTDAGYNGVGTALVAPNGRAVPTFRIRYTVGNAVRQSECELFASYVKN
                     LGINVSVEPTDSLGTTTSTGDFDIIVFAWVQSPFVFGGAQQLWLSTSGSNYGKYNNPQ
                     VDQLINEAAASTDAASAINQLNQADKIMSDDAYVLPLYQKPTFVAVQDSVANVRNNSS
                     LDGPPYNAAEWGLRSQ"
     gene            complement(19045..20874)
                     /locus_tag="VFE39_06075"
     CDS             complement(19045..20874)
                     /locus_tag="VFE39_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6258202.1"
                     /translation="MRAPLARRTGAALLLLLAVLLAGCTNAPVQPPPPAPAPTPPPAP
                     TRLVVGVGELGAGFNPHLLADLSPVTTALATLVLPSVFHPDANGVMQLDPTVATSAQV
                     VSTAPFTVSYELNLEASWSSNAPIAAEDFVYLWEQMRSQPGVADAAGYRLITDVRSRA
                     GGKAVDVVFSQAYPAWKTLFSDLLPAAILKDAPGSWTGALTGGLPASGGPFRISSVDR
                     ARGEIVLDRNDLYWDTPTVLDELVLRRMDGPVLADGLASGEVNVALPESTPAIRQLLG
                     GLQPAPRLQPAPEPVVTQLGMRADTGPLADPRARRGIALLVDREAVRAAVAPAALPAD
                     SFGLAPSQPGYGSTAPPDAPLRPNPVAAAQALAAAGWTRDPTTGRWAVDGAPVQLVLA
                     AAAERPEDVRVAQTIAAQLEAAGVGVTVIAPPGAQLFAQQVVPAVPPTPTATPTPTPT
                     PLPSSAAPPARPSRVAPPAPRATPTASPTTTPPAPPGDVEADMMILPRTVGGDLGTEL
                     ASDYGCPASTALVPDPPRSPTGFCFPALQPLFDELLSAAPRPTTAGVVEGVLWAQLPV
                     LPLFQPVDLVVSTPAADAATHVGPGPLQTGPLTGAQRWRGPNE"
     gene            complement(20969..>21289)
                     /locus_tag="VFE39_06080"
     CDS             complement(20969..>21289)
                     /locus_tag="VFE39_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET6258203.1"
                     /translation="ALADRGVLFVGPGTIVYGGMVIGEYTRNEDLEVNIVREKKLTNM
                     RKSTADELVKLTPPTVLNLEQALEFCANDECVEVTPESVRIRKVELDSHTRARMRSRA
                     RSVS"
BASE COUNT         3146 a         7577 c         7606 g         2960 t
ORIGIN      
        1 gagcagcccg tcgcgttcca ccccggcccg gtccagctcg tcggccagcc ggtcgagcac
       61 ccagtccacc tcgtccatcc ggtagccgcg cagcgcctgt tggaaccgca gctcgcggac
      121 gtcctcgccg tcgagctcac cggccggcaa ccgcgtgggc gtggcgccgg gaggcagagg
      181 ggccagctcc tcgccgcgcc cgaacacgac cgcggccagc ccgaagaaca ccgcggccac
      241 ggcggcgacg atcatcagat agatcagcgc ggtccccacc tgggcaatcc caccacgcgt
      301 gtccggtcag cgcaccagca cctcacgcat cggtggccgc tcggtgccgc cgatcgcggt
      361 ggatgacggc agccactcgc cgtcgacctg caggaactgc gtgagcgccg ctcctgtgtc
      421 gggtataccg caccggctga gcgccgccgc gaggatctgc cgggccatca ccccgagctg
      481 caacagcgat cggttgcggt gcttgcgcac gccgaggttg acctgcgcca gggcgtccag
      541 cccgagtgtg gcgtacgtgt cgagcagcag tccgatctcc acgccgtacc cggccgcgaa
      601 cggcaccgac tccagcagct cccgcgttcc ggcgtactcc ccgccgagcg gctggacgat
      661 gcccgcgagc tcgggtcgca gcgccgcgag cagcggccgc acgaggagct ccgtgacccg
      721 accgccaccc gtgtcggcct ccgtggtctc cagccgcagg gggcgccggt agaacccctt
      781 gacgagtgcg acgcccggtt cggtgagtaa cggaccgagc agcgccggca cgaacccggc
      841 gtcgaagtcg accaggtcag agtcgaggta gcagatgacg tcaccggtgg tggcggcgag
      901 cgagcgccag agcacctcgc ccttgccggg cacgggcggg agctccgtaa ggacgtcggt
      961 gcgcagcacc accgtggccc cggccgccgc tgccaactcg acggtgcggt cggtcgaacc
     1021 ggaatccatc acgaccagct cgtccaccaa tggcaccgta cccagcgtca gcggcacgat
     1081 cgccgcgacg atcgcgccga ctgtggcttc ctcgtcgagc gcgggaagca cgacggacac
     1141 ccggcggtgc gccttcgccg ccaccaactc gtcgatgctc cagccgggtt cctgccaggt
     1201 gcgagggccg aaccactcct cggtgacgcg ctccatgggc ctctcctacg cggtcgaagg
     1261 gcggccccat tgccgatgac gtcagggtca cgccagggcg cggaccgttc gggcaggtgg
     1321 gtgggtaccc gcgatcgcgg ccaccatgtc gacggtatgg cgggtcgcgg ccacctcgtg
     1381 ggcgcggaac acgcgcgcac cggcggctgc ggccaacgcc gtggcggcga ggctgccctc
     1441 gagccgctcg gtcacctccg cgacacccag cgtctcgccg acgaagtcct tgttggagag
     1501 cgccatgagg accggccagc cggcggccac cagctcgtcg cagcgtcgca gcagggtgag
     1561 cccgtggaag gtgttcttcc cgaagtcgtg cgtcggatcg acgaggatgc cttcccggcg
     1621 cacgccgagc gccacgagcc gctcggcctg gccgcagacg tcggccagca catcggccac
     1681 gacgtcggcg taccgcaccc ggtgcggtcg gctccggggc aacgctccgc cagtgtgcga
     1741 gcacacgatg cccaccccgg tctcggcggc cacgtccccc agtgccgggt cggcaccggc
     1801 ccacgtgtcg ttgagcaggt cggcgccctc ggcgacggcc agcctgccga cctcaccacg
     1861 ccaggtgtcc acgctgatca cgacgtccgg gtagcgctcg cgcaccgctg cgacgaacgg
     1921 gacgacgcgg cgaacctcct cggccacatc gacatcgggg cccggccccg ccttcacacc
     1981 cccgatgtcg atgatgtccg cgccctcggc gagcgcggcg tcgaccttgg ccatcgccgc
     2041 accgtccgcg aaggtggcac cgcggtcgta gaacgagtcg ggcgtgcggt tcacgatcgc
     2101 catcaccagc gcacggtcct gtgccggttc ccgcttcccg aaccggatca cgcccaccgc
     2161 gcccgtcacg tccgggatgg tgacatgtcg accgggctgg cgcaggcgtc gagggcggcg
     2221 gccacgtcgg tgacgaccac aagccgcgcc agcgcctccg gccggacgaa cccacgctcc
     2281 cccagcccgc gcacccagtc cagcaggccc gtgtagtgcc cgtccgggtc gagcagcacg
     2341 gttggcttgg cgtgcatgcg caggtagccc gccgtccaca cctcgaagag ctcctcgcac
     2401 gtcccgatgc cgccgggcag ggcgaggaac gcgtcggcgt gcgcctccat cagcgccttg
     2461 cgctcgcgca tggtgtcgac gaggagcagc tcgtccgagt cgtggtcggc ccattccagg
     2521 tccaccagcg cgcgtgggat gactccggtg gtgcgcgccc cgcccgccct ggccgcggcg
     2581 ccgatcgcgc ccatcatcgc cgcccgccca ccgccggaca ccagctccca gccgcgggtg
     2641 gcgatctgcc ggcccacctc cgcggcgagc tcgaggtaat ggggcgcgat gccctcgatg
     2701 gatgcgcagt agacgcagac agcgaccgaa cgcccggtca atgcgcttcc tcccacgccc
     2761 gccacgcctc ctgcacgatc ttcacggcgt cgtcgacgtc gtcggtgacg tgcaggaggg
     2821 cgaggtcctt gtccccgacc ttgccgctcg cgtgcacggt gttcgcgatc cactcgtaga
     2881 gcccacccca gtactcgctg cccaggagca ccacggggaa cttcgtgacc ttcttcgtct
     2941 gcaccagggt gagcgcctcg aacagctcgt cgagcgtgcc gaagcccccg ggcaggcaga
     3001 cgaacgcctg cgagtacttg acgaacatcg tcttgcgggc gaagaagtac cggaagttga
     3061 tgccgaggtc gacccagttg ttgaggccct gctcgaaagg cagctcgatg ccgagcccga
     3121 ccgagagtcc gcccgcctcg gagcagcccc tgttggcggc ttccatcacg ccggggccac
     3181 cgccggtgat caccgcgaac ccggcctcgg ccagcgcggt gcccagcgcc aggccctggg
     3241 cgtactcggg gtggtcgggc ccggtgcgtg ccgagccgaa cacggtgacg gcacggggga
     3301 gctcggccag cgtgccgaag ccctcgacga actcggcctg aatgcgcagc acccgccagg
     3361 ggtcggtgtg cacccagtcg ctggggccac gcgagtccag caaccgctgg tcggtggtgg
     3421 aggactcgtt gcggtactcg cgccgcagca ccacgggacc ccgttgcttc tgctcgggct
     3481 gcttgtcgtc gggacgccgg ttcccgctgg cggtcatggt cgccagagta gtgagaccgg
     3541 cacggaccgc tcggccccct gtccccaggc ggagtgacat tgctgacatc gccgacgccc
     3601 gcgatgcgct cagggcgaaa ggaagcggcg cagaaccgtg gcggtgtcgg tgatctggcg
     3661 ggtgtggacg tgctcctcgc gggtgtgggc gacattcggg tcaccggggc cgtagttcac
     3721 cgcggggatg ccgagcgcgg cgaagcgcga gacgtcggtc cagccgtact tcgccgacgg
     3781 cgtggcaccg gtcgcggcga tgaattcgcc ggcggcaggc gcggtgagcc cgggaagcgc
     3841 gcccggcgcg agatcggtga cggcgagctc gaacccgtcg aacacgtcgc gcacgtgggc
     3901 ctccgccgcc gcgctgtcgc ggtcgggggc gaaccggaag ttgaccgtga cgacgcactc
     3961 gtcgggcacc acgttcatgg ccacgccccc ggcgatccgc accgcctgca tcccctcacg
     4021 gaagacgcag ccgtcgatgt cgacctcgcg ggcggtgtag gtgttcagcc gctccagcac
     4081 cgcggcggcg gcgtgcaccg cgttgcgacc gtgccacgac cgagccgagt gggcacgccg
     4141 cccacgggtg gtgatctcgg cgcgcagggt tccctggcac ccggcctcca cgcccgcgtt
     4201 ggtcggctcg gccaggatcg cgaggtccgc gtgcagccag tctcggtgct cgcgctggat
     4261 gcggttcagc ccgttgcggt cggcctccac ctcctcgcag tcgtagaaca cgagcgtgag
     4321 gtcgtggcgc ggctcggtga gggtggccgc aaggtgggcg agaaccgcgt cgccggactt
     4381 catgtccgtg gtgccgcagc cgtagagcag gtcgccgtcg cgcctgctgg gcacgttgtc
     4441 ggcgatcggc acggtgtcga ggtgacccgc cagcagcacc cgccgctccc gtccgaggtt
     4501 cgtcctggcg agcacggcgt cgccgcagcg gagcagttcc aagtggctag cctgcttccg
     4561 cagcgccagc tcgacggcat cggccagcgt cgcctcgtcg ccggagacgc tcggcacgtc
     4621 gaccagcgca gcgcacagtt cgacggggtc ggaggtgagg tccagcacgg ggatcgtcac
     4681 gcccgacgac gctacccggc gacggcgcct gcgaccgccc ggcgatcccc cgcccaggcc
     4741 gcgggtgtgg ggcgcgggcg gataccgtgg gatccgtgag caccgcaccg cgccgggagg
     4801 gcgcctccgg taccggcctc gccaccgtca ccgtgcccgt cgacggcagc gggggcgtcg
     4861 ttctcgacac gtggttccct gcaccggcgc tcggcggtca ggccccggac ggcgcagccg
     4921 atctcgagca cctcatcggc accgacgagg cccggggcgt gcgcaccgtg gtggtccgca
     4981 cggccatcga ctcgctcgcc gacccgcccg ccgacccgta cgacgtctac ctgcggctgc
     5041 acctgctcag ccaccgcctg gtcgcccccc gcgaggtgaa cctcgacggg atcttcgggc
     5101 tgctcgccaa cgtggtctgg accgaccacg ggccgtgcgc agcgccgggc ttcgagctca
     5161 cccgcgcccg gctgcgcgcc cgcgggccgg tcactgtgct cggtgtggac aagttcccgc
     5221 ggatggtcga ctacgtgctc cccaccggcg tccggatcgc cgacgccgac cgggtccggc
     5281 tgggtgcgca cctcgccgaa ggcaccaccg tcatgcacga gggtttcgtc aactacaacg
     5341 cgggcacgct cggagcgtcg atggtcgagg ggcggatctc ggccggcgtc gtcgtcggtg
     5401 atggctccga cgtcggcggc ggcgcatcga tcatgggcac cctctccggc ggcggcaagg
     5461 aggtggtgtc ggtgggcaag cggtgcctgg tcggggccaa cgccggaatc ggcatctcgc
     5521 tcggtgacga ctgcgtcgtc gaggccgggc tgtacgtcac cgcaggcacc aaggtcgtcc
     5581 tcccgtccgg ggagacggcc gccgcccgca ccctttccgg gcagaacggg atgctgctgc
     5641 gccgcaactc ggtgaccggc gcagtcgagg tgctggcccg caccggcgac ggcatcgctc
     5701 tcaacgccgc cctgcacacc aactgacggc tggatcgcac gtagtcttca cggtcgtgcc
     5761 cactctggct tcgcggcgct tgcagcagct ccccttcgac gtggcggacc tgccggcgcc
     5821 gctttccgtc acaccgtccg acccacccgc gcagcacgtg cgagcggcgc tcgacctgcg
     5881 gctgcgcgcc ctgctgtacc acgacccggg cacccggagc ggcgcggaca tcgaggacct
     5941 gcatcagatg cgggtcgcgg tgcgccggat gcgggccgca ctcaaggcgg ccaggccgct
     6001 gctcgactcc gcgtgggccg acggcctgcg tgccgagctg ggctggctcg ggcgggcgct
     6061 cggtcctgtc cgggacctcg acgtcatgct gctccggctg cgccgggagg tcgcctctct
     6121 cccggagcgg gagcgggcgg cgggcggagt gctcgtcgac tcgatggagg gcgagcacgc
     6181 ggcagctcgt gcactgatgc tggccaccct cgatgcgccc cggtacaccg cgctgctgga
     6241 acgcctcgcc gacgccatcc ggctgcccct gcccaccccg tcggccaccc gggcccaacc
     6301 cgagctcgtc gatctggtgc gcgccgaggc ccacaggatg cgcagggccg tggagaaggc
     6361 cggggcggac ccgccggacg ccgttctgca cgagctgcgc atcttcggca agcggttgcg
     6421 ctacacggtg gagctggtcg agccggggct gcggggtcag gcgctgcagg gccgttccga
     6481 ggcgggacag ggacgagcgg tcaagcgact gctcgctgcc acggcacagc tccaggaggt
     6541 gctgggcgac caccaggatg cctgtgtggc cgagcagcgc atccgcgagc tgctcgacgg
     6601 cctcggcgag ctccccgacg cgcacgtggt gttcgtggcc ggtcgcctgg tggaacgcga
     6661 gagggcgaga gcggaacaca agcgagccga atggccgtcg gcctggacac aggtcgccga
     6721 acggatcgcc gacttctgac ggactccgcg ccggaggtgc ggctcagtgg gcggggcggt
     6781 cgccgatccg ccaggccgcc caaccctgga gggcggctgc cgccagcagc accgcgagtg
     6841 ccgcagagaa gcccttcgtc aggtcgatca gcgcgcccat gaccagcgga cccaggcctg
     6901 cgctgaggta accgacgccg agggccagcc cgctcgtggc cgcgctggcg gcgccgtccg
     6961 cggtgcgcca tgcgatcacc gtgaggccga gggggaagcc cgccccgacc ccgatcccga
     7021 tcagcaccgc ccacagccag gggcctgcca ccggcagcgt gggcagcacc atcgcgccga
     7081 gcgtgcccgc cgccccggcg ccgagtgtca gcccggacca gaaccgccag cgccgtcggc
     7141 gttccgcgag cgccgggacg agcagggccg cggggatctg cgcgacgctc cacacggcga
     7201 gcagcaggcc cgcgcggtgc gagctccagc cctggccctc gtagtagggc gccagccagg
     7261 tgagccagcc gtagaacatg agcgacgtgc cggcctggta gcaggccacc gtccgcgcga
     7321 acccggagcg ccacggcagc gacaggcgcg gggctgcgcg gtcgggagcg cccgtccggc
     7381 gggcaaccgg cacccagatc gccacggcca gcacggccgg gacggcccac acggccaggg
     7441 agcgcgacca accccccaac gccgcggcca acggcaccgc gacggcgctg gcgaccgtcg
     7501 ctccgaccat catcgagacc acgtagccac cggtcgcggt accggcgcgc tccgcgaggt
     7561 gctccttgac cacgccggcc agcagcaccc ccgccaaccc gatcccgagc ccgatgagca
     7621 ggctgccccc gatcagcggc agcagcgccg gtacgccccg caccatgctg cccagagcca
     7681 ccaacccgac ggagccgccg agcgcgcgct cccgcccgaa ccgcgccccc acagcggacc
     7741 cggcgaacga cccgaccgcc atcatcagga cggcaccggc ctgcacgaaa ccggccacgg
     7801 ctgccgacgt accgagctcg gcacgcaccg tctcgagcag cggcgggtaa ccggccagca
     7861 ccgcccggag gttgaccgcg aggacgagca acccgagcag cacgacaccg ggccggaccc
     7921 ctcgcgtccc ggcccgcctg gacgacgtcg tccccgagcc ggaggtcacg cttcgagcaa
     7981 ccgctccgct gccgccgcga tccgctcgtc ggtcgccgtc agggccacgc gcacgtgctg
     8041 cgcccccgcc ggaccgtaga actctccggg tgccaccagg acaccctgcg tcgccaaccg
     8101 ccgcacggtc aaccgggacg gctcacctgc ggtggcccac aggtacagcc ccgcctcgga
     8161 gtggtccacc cgcagcccag cagcctcgag cgtgacccgc agccgggccc ggcggcgggt
     8221 gtagacctcg gcctgcgcca ccacgtgggt gtcgtcggag gccgcggcgg tcatcgccgc
     8281 ctgcaccggc cgcggcacca tcatgccggc gtgcttgcgc acctccagca ggtcggcgac
     8341 cagcgacggg tcccccgtga cgaaaccgcc gcggtagccg gccatgttcg aggtcttgga
     8401 catggaatgc acggcgagca ggttctcgaa ggagccacca cagacgtcgg ggtgcagcac
     8461 ggagaccgcg cgttgtgagg cgggggccag tgacaggtag cactcgtcgg acgccaccac
     8521 cgctccacgt tcacgggccc agtcgacgac cttgcgcagg tgctccgggg gcagcacccg
     8581 tccggtgggg ttggacggag agttcagcca ggtcagcgcg gtgcgctgcg ggccgagcgc
     8641 cgccgtcgag tcggtgcgga cgaactccgt gcccgcgagc cgggcaccga cctcgtacgt
     8701 cggataggcg agctcgggga tcacaaccgt gtcgcccggc ccgagcccca gcagcgtggg
     8761 cagccacgcg acgagctcct tggacccgat cgtcggcagc accgcgaccg ggtcgaccgt
     8821 cacgccgaac cgccgggcca tggcgccggc gatcgcctcg cgcaacgagt cgggcccgtg
     8881 ggttgtcggg tagcccggct cgtgggcggc ggggccggcc agagcagcgc gcacgacgtc
     8941 cgggacgggg tcgaccgggg tgccaaccga gagatcgacg atcccgcccg gatgggccgc
     9001 ggcgagcgcc ttgtcctcgg cgagggaatc ccagggaaag tcaggaaggt cgagcttcat
     9061 aacgcactgc gggtgaccac aaagcggtcc tcactcgtcg tgctgctgag gcggcaggct
     9121 cgcggcaggc tcgacgtcgc ggtcgatctt gccgatcttc gacgcaccgc cgggcgaacc
     9181 gagctcgtcg aagaagtcga cgttcgcctt cgtgtacgcg ctccactgct cggggacgtc
     9241 gtcctcgtag tagatcgcct cgacggggca gacgggctcg cacgcaccgc agtcgacgca
     9301 ctcgtcggga tggatgtaga gcatccggcc gccctcgtag atgcagtcca ccgggcactc
     9361 ttcgatgcac gccttgtcga ggaggtcgac gcagggttcg gcgatgacgt aggtcacggg
     9421 tggtccttca ctcgggcgtt cacggcaggt accagtatgt ctcgggagct gatccgaccg
     9481 ggagccgggg cggtgtgaca ccgctcgtac cggcgatggg acgtggacca ggatggtccg
     9541 gatggagatc tccgacgagc tggtaggcgc ccgggtggca ctccggtatc ggatcggcca
     9601 gcgtgacggc cgaccgctct acagcgacgc ggtgggcgag ctgagtgcgg ccggtcctga
     9661 tgccgtgctc gtgcagaccc ggcgtggccc ggtccacgtc gaccgcgcgg ccgtggtcgc
     9721 cgtgcgcgcg gttccgcccg cgcctgcgcg ccggccgtcg ttgaccgcga tcacgcggct
     9781 ggaaggtctc tccgccgacg cctggcccgc cctggtcgac cggccgctcg gcgcctggcg
     9841 gctgcgcgca gcaggcggct acaccggccg cgccaatgcc ggtctggtgc tcggcgaccc
     9901 ggggatgccc atcccggccg cgctcgacgc ggtgcgcgcg ttcgcccacg agcacgagat
     9961 cccggcgcgg gtgcacgtcc cgatcggctc gccgtgggac cgggcggtgg cggcgcaggg
    10021 ctgggtgctc gacgtcgggc acgctgcggg cgcggaggtg gcagtgctcg ttgggcgcct
    10081 ggcggggatc cgcgacccgc gcgtggggct gcccgagcgc ccgtccgacg catggtggca
    10141 gctcgcgctg ggcgggcccc cgacgccggc gcagcggcac gtcctggatc cgggcgggca
    10201 cccgcccacc gcgttcgccg agatcccggg accggacggg gccgcgaccg cccaggtccg
    10261 ggccacggtc gtcgaggacc acctgcacgt gtccaagctg gaggtcatac cggcagcgcg
    10321 gcggcaaggt ctcgcgacgg ccctgctcgc ctcggtggcc gagtggggcc gggagtacgg
    10381 cgcacggtgg gcagtgctgc aggtgccact gcagaactcc ggcgctcgtg cgctgtacgg
    10441 ccgcctcggc tacgtcgagc accaccagta ccgatacctg gtgcctccgc ccggctgata
    10501 ccggccgtgc gcggaaacgg gtgccggagc actcgtattc actcacgggg gtggcgcgca
    10561 cgaacggccg gagcacggcg acgaccgccg cgtccgggat gtggtcagcc accggtcctc
    10621 tgatgctccg tgttcagcac gccccgcagc gcccacaacc cggacccgac gccgccgacg
    10681 accaggagga gcgactgcca cgtggccggc agcatgatgt cgccgcccgg tccgacgagg
    10741 ccgagcaccg cgacggtgag cacccacgcc gacagcggcg ccgccgctcg ggacggccgc
    10801 gcgtcgacct cgcctgcccg gcgcacgagc cacggcagga tcaggaccga gagcacgacg
    10861 ccgatcggga ccggcacgcc acccgcgtac agcggcgtga gcaccagccc caacccgccc
    10921 agcagcacgc cgtcgaggat gagcagcccg aacaaccccc cgtccgacac cgcgggcggt
    10981 tccggctcac ccatggtcgc cacccgcgaa caggtcggcc gccaacggat cgcctgcacc
    11041 gtcggccgcc acgtactcct ccacgtcgag cagcggttgt gcgacgaggt tgctcatggc
    11101 cagcgcgatc gcgtcgccgc tctcccagag ggcgatctgg gtggcgtgcg cgcgcatggc
    11161 cgcgatccgc gccgcgcgct gcgccgtgac gtcgacgcgc acggtgatcg cagcatccgg
    11221 cacgctgggc agctcgtcgg gcccgggcaa gcggaacggc agccccggga cggccgccag
    11281 cgcggcacgg ccggcgtcga gcgccccgcg accgatcacc gtgaagtaga gcctggccgg
    11341 cacccgccgt agcgcggcca cggtgatctc gtgggcgcgc acgtggtcgg ggtggccgta
    11401 gccgccgtcg gccgcgtagg tcaccaccac ctggggacgc acctcctcca gcacggcgac
    11461 gagcgtgtcc acctgctcgt cgaacgcctc cggccgggcg aacgcccggg gatgcaggtg
    11521 ctccggcgtg gcggcccgga cgccgtgccc ggcgagcgcc atccccgagt cgcgccagcg
    11581 cccggcccca ccgaggaagc ggtggtcgcg cactccgagc gcggtacagg cgcgcgcgag
    11641 ctcgccgatc cggtagccac cgagctggtc ggccttgccg ggagcgagga gcgcgagatc
    11701 ctgcgggatc acctcgccct gctcgcccag ggtgcaggtc accaaggtga cggatgtgtc
    11761 ctgctccgcg gcgtagcgcg cgaccgtgcc tccggtggcg agcgtctcgt cgtccgggtg
    11821 agcgtgcacc aggagcaacc ggcgccgggg agcgggcgtc acgagcgcac tctacgtgcg
    11881 ccgcagacgt gcccgatgtg cactccaccg cccggatcac cgcattcacc cgtctctccg
    11941 tgatcgcccc atagatcagt aggtgagcgg tcgcaaccga cttgtcatct ttgcgcgcct
    12001 cgccgtacga gtgagacggc tactctccgc acacaaacct caccgtgtgc gacacaggtc
    12061 accccttcgt gtcgctgtca tggatttggg agcccatggg gagctgcggc ggaaggtgca
    12121 ggaactcctc gacgtcgtcg gactcaaccc cgagcacatc aaccggttcc cgcaccagtt
    12181 ctccggtggg caacgccagc gcatcggcat cgcccgggct ctcgccctgc gtcccgaggt
    12241 ggtgatctgc gacgagccgg tatccgcact ggacgtgtcg atccaggcgc aggtatgaac
    12301 ctgctcggcg acctgcaggc cgagttcggg ctgtcctatc tgttcatcgc ccacgacctg
    12361 tccgtcgtcc ggcatctgtc ggaccgggtg gcggtcatgt acctgggcag aatcgtcgag
    12421 atcgggacgg aggacgagat ctacaaccgg ccctcgcacc cgtacacgca ggcgctgctc
    12481 tccgccgtgc cggtgccgga cccatccgtt cgcctcgagc gccagcggat ccgcctcacc
    12541 ggcgacgtcc cgagcccggt caacccgccc agcggctgtc gcttccggac acggtgctgg
    12601 aaggccactg agatatgcgc ggaggaggag ccggccctcg aggtgcgacc gcccggccac
    12661 cacccgagcg cctgccacca cgccgaggag cagcacctgc ttcccggctg accaggcagg
    12721 tcagatcagg accttgcgct ccgcgtagtg gcacgcggcc ggatgaccct gaccacggtc
    12781 caccagctcc gggaccacgt ccacgcaccg gccgcgctcg gcgtcgctca gctcggcggc
    12841 gaacttggga cagcgggtgc ggaaccggca cccgctgggc gggtcggccg ggctcggcac
    12901 gtcaccgacc acggtgatcc gctccctggc ccgctgcttg cgcggatcgg gcagcgggat
    12961 cgccgagatc agcgcctggg tgtacgggtg cgccgggccc tcgaacagct cgctcgcggt
    13021 gcccgtctcc acgatcctgc ctaggtacat cacggcgatc cggtcagcgg tgtggcgcac
    13081 cacggacagg tcgtgcgaga cgaacaggta ggacaacccc agctcgctct gcagctcgtc
    13141 gagcaggttg agcacgccgg cctggatcga cacatcgagt gcggacaccg gctcgtcgag
    13201 cacgagcacc ttgggtcgga gcgcgagcgc ccgcgcgacc ccgatccgct gtcgctgacc
    13261 cccggagaac tcgtgcggga accggttgcc gtgctcgggc ttgaggccca cggtgcgcag
    13321 caggtcccgg acctgcgttt tcccggcgtc gtcgtagcgg ccgtggatcc gcagcggctc
    13381 ggccacgatc tcgttcaccg gcatccgcgg gtcgagggac gcgtaggggt cctggaacac
    13441 gatctgcagg tcgcggcgca actcgcgcat cttcttggac ggaagggtgg tgaggtcagt
    13501 gccgttgtag aagacctggc cggaagtcgc ctcgatgagg ttcaggacca gccgtgccgt
    13561 ggtcgacttg ccgcagcccg actcgccgac cagccccagc gtctcgccgg gggtgaggtc
    13621 gaacgacacg tcgcagacgg cgtgcaccac gccgacctga cgccggacga gacccgtcga
    13681 gcggatcggg aagtgtttga cgaggttgcg cgccgacagg atcggctcgg gatcggtagc
    13741 ggccgaaccg ctctcgtgca tggccggtgc gctcatcgct cgccctcgct gcgctcggcc
    13801 ggcccaccat gagacgtcac agcgcgcagg cgctgcgtcg atgactcgct tgcaagctcg
    13861 ctcatgcctg cccctcggtc tttctcacga agtgtgcgag cgcctcggtg tcgacgacat
    13921 cggtctcgaa cacgtcgccg ggggtggccc cgactagggc atcggagcgg tggcacgcgg
    13981 cgaggtgctc cggccccgtg gtcggcgcga gcgccggctc gacctcctcg cagacggcga
    14041 cggacagcgg gcagcgcggc gtgaacggac agcccggagg catgttgacc accgacggag
    14101 gcgcgccctg gatcggtgtg agccgctcgc tgggccggtc gagccggggc aggctgccca
    14161 gcagcccgaa ggtgtagggc atccgcgggg tgtagaagac gtcctccgcc gtgccgacct
    14221 ccaccagctt gcccgcgtac atgaccatca cccggtccgc ctgtccggcg atgactccga
    14281 ggtcgtgcgt gatcagcacc atcgccgcac cggtctcctg ctgcgccagc tggagggact
    14341 ccatgatctg tgcctgcacc gtgacgtcga gcgcggtggt cggctcgtcg gcgatgatca
    14401 cgtcggggtt gttggccatc gcgatcgcga tcaccacccg ctggcgcatg ccgcccgaca
    14461 tctcgtgcgg gtagttctcg agccgctggc gcgggttcgg gatgcgtacg agctcgagca
    14521 gctcggcggc gcggtccagg gcagccgcct tgctgacgtt gtggtgcgct cggacggcct
    14581 cggccacctg gaacccgatc gagtagaccg ggttcagtga cgtcatcggg tcctggaaga
    14641 tcatcgcgac gttgcggcca cggaccttgt tcaactcggc ctcgggggcc cgcagcagct
    14701 ccttgccgcc gaaacgcacc gagcccgaca ccttcgcgtt gccgggaagc aggcccatca
    14761 cggcgagtga ggtgaccgac ttgccggagc cggactcgcc gacgattccg aggacctcgc
    14821 cccggcgcag cccgtagctg accccgcgga ccgcctggac cagcccgtcg tcgctcggga
    14881 acccgacagt gaggtcgctg acctccagca cgacgtcgga cgccgcaccg ttcctcggca
    14941 ttgtcggggt gatcgagtca gcgagggggt cctccgtcac tggcgcacct tcctctgctg
    15001 cgggtcgaac gcgtcacgca ggccgtcacc gatgaagttg atggtcagcg cgatgacgat
    15061 gatgaacagg ccggggaagt agaacagcca cggccgagtg gtgagcgcgg tctgcgcctc
    15121 gctgaccagc aggccgagcg aggtgtcggg cgtctgcacg ccgaagccca ggtacgacag
    15181 cgccgtctcg agcaggatcg ccagcgcgac gaggatcgtc aggttgacca ggatcgagcc
    15241 gagcgcgttg ggcacgaggt ggcgcaggat gatccgggca tcggtggcgc ccagcgcctt
    15301 cgccgcctcg atgtactcct tctcccgcag cgagagcacc acaccgcggg acacgcgcga
    15361 cacgtacggt gcgtacagca ccgcgatgac catcgcgatg aggtaccagg tgccgccgaa
    15421 gttgtgggcc agcatcgccg ccaccgcgat cagcggcagg gtcaggacga ggtcggcgag
    15481 ccgcatgagc gtggagtcgg cgcggccgcc gaaatagccg gccaccgcgc cccagacggt
    15541 gccgaccacg gtggcgaaga ccgcgacgag cacggccacc tggagggaga tccgggtgcc
    15601 gcgcagcacc tgtgcgaact ggtccttgcc cacggcgtcg gtgccgaacg ggtggtcgaa
    15661 cgacggcggc tgcgagttgt ccggagtaat ctcgcccgcg tggtacttcc acagcgcgcc
    15721 gcccacgtag gcgagcagca ccacgaggac caggacgacg aggctgatca tcgcggcccg
    15781 gtgctgcagg aaccgccgca gcaccagctg cgcctggctg cgctcgccga cggtgaactc
    15841 gcgctcggcc gcaggcaccc gtccggtgcc cggggccggt aggggcgagg tttcgggcgt
    15901 cggggctacc tcacgcatag cggatcctgg ggtcgaggac gccgtagagc aggtcggcga
    15961 tcaggttgaa cacgacgacg atgacaccgg tgaggagcag ccaggacgag gcggcgtaga
    16021 cgtcctcggt gcggatggcg ttcagcaaga agtcacccat ccccttccac tggaacacgg
    16081 tctccgtgac aacggcgcca ccgatgatcg acgcgatgtc gagcgcggtg accgtggtca
    16141 acgggatgag cgcggtgcga agcgcgtgct tgaccatgac agtgctgcgc gagagccctt
    16201 tcgaccgggc cagccgcaca tagtcgctgt tcatcacctc gagcatggat gcccgcgtga
    16261 accggctcca ggacgcgtag gagatgaggg cgagcgcgat ggtgggcagc accatgtgcc
    16321 cgagcacatc gacgatctgc gcgccggtgc cgggtggtgg gggtatcgac gtcgccccga
    16381 tcgtgtagac cacctgcgca tcgacggcct tgttgaactc gatcccgccc tgcttgagca
    16441 gaatggccag ccagaacgac ggcatggcga ggaacaggaa gcccaggaac gtggtggtgt
    16501 agtcggtgac cgtgtactgc ttcacggcgc tgagcacccc cacgacaacc gcgaggacca
    16561 gcgcgatcac catcgcgagc gcgatcagcc ggatcgtcac ccagaaccgc gcgccgagct
    16621 cggagccgat gttcacggtg gagacgacgg agggcccgaa gtcaccgtgc agcagtccgg
    16681 cgagccagga gaagtagcgc gggatcaacg gctggtcgag gtgcaggcgc accgtctcgg
    16741 cggcgatgac gttgggtggc gccggtgggt tcttcgccag taggggagcc agtgggttgc
    16801 cggagctgac gcccagcgcg aacacgatga acgaggcaag cagcaggatc gggatcgaga
    16861 tcgcgatccg gcgcacggcg aaggcgagca tggaggttgt cctgcttctc ctgatttgtg
    16921 gcgagatggg tccgaagctg cttctagcgg cgacgagacg ggagttggcc cagcgtaaac
    16981 gggccgtggg ccgggtcccg aacggcgtcc gccgttcggg ggccggccca cgatcactgt
    17041 gctgccacgg tcactgcgtg cgcagatttc actgcgaccg caggccccat tcggcggcgt
    17101 tgtagggcgg gccgtcgagc gaggagttgt tgcggacgtt ggcgacgctg tcctgcaccg
    17161 cgacgaaggt cggcttctgg tagagcggca ggacgtaggc gtcgtccgac atgatcttgt
    17221 cggcctggtt cagctggttg atcgccgatg cggcgtcggt ggacgcggcc gcctcgttga
    17281 tcagctggtc gacctgcggg ttgttgtact ttccgtagtt cgaccccgag gtggacagcc
    17341 acagctgctg ggcaccgccg aacacgaacg gcgactggac ccaggcgaac acgatgatgt
    17401 cgaagtcacc cgtgctcgtc gtggtgccca gcgagtcggt cggctcgacg ctgacgttga
    17461 tgccgagatt cttcacgtac gaggcgaaca gctcgcactc gctctgccgc accgcgttgc
    17521 cgacggtgta gcggatgcgg aaggtcggca cggcccggcc gttgggcgcc acgagggcgg
    17581 tgcccacgcc gttgtagccg gcgtcggtca ggatcttctt ggccgcgtcg atgttgcccg
    17641 agccctggcc ggtgggggtg atgttgtcgg tgtagccgtc ctgctgcggg atgaacatgt
    17701 ggttgttcag cggcttcacg tcgtgattga actggccgac ggtcttggcg atgatgtcct
    17761 gtgtgttgac ggcggtgtac atcgcctggc gcagcgcctt gtcggcgagg aacgggttga
    17821 ccaggttgaa gtcgaagtgc tcccaggtca gccccagacc ctgggtctgc gacacgttcg
    17881 ggatctgcgc cacctggctc accaggtcga cctgcggctg cgggtagatg acctggacct
    17941 cgttgttctg cagcgcgagc ggctcctggg tggcgtcggt gatgacgcgg aacaccaggc
    18001 ggtcgagctt gggcttggtg ccccaccatg ccgggttcgg caccatcgtc agcgccacgt
    18061 tgggctgcca gttgtcgacc ttgaaggggc cggccgagta gttgatcacg gtcgagccga
    18121 ggtagtcgaa ggccgacttg aggccggccg gagtgttcag gtcgccatgc tgcgcggcga
    18181 tgtgcgccgg gtagaggggc gcggaggagt tgaagatgct cttccagtcc gtgtacggct
    18241 tggaccagtt gaccgtgacg gtcttgccgc cgtccgatcc cacgacgctg gtgatctggt
    18301 cgtacccacc gttgcccgcg gtctcgcagt cggggcagtc gcggccgttc tgggtcttcc
    18361 agttgaagac gaagtcgtcg gccgtgaccg gggtgccgtc gtcccacttc gcctgcgggt
    18421 tgatcttgta cgtgatgacg aacgggctcg tgctggtcac ctcggccgaa gtgacgtagt
    18481 cggggttcat cgtcgacttc aggtcgggcg tcgtgatgaa ggcgtacggc agtagcggct
    18541 tcagcgccat gctcgtctcg aagacgttgc cctcggagga gttcaggttc cagttgtcga
    18601 tgttcttctc gatcgcgaag ctgatctgcc cgccctgctg caactggtcg gccggcaccg
    18661 cgttgcaggt gttcgcatcg gcgcagttct ggaagagacc cgtcgacgtc gacgagccgc
    18721 cgccacctcc accaccgcca ccgccaccgc aggcggcgag caccagcgcc gccgccgcag
    18781 acatcgcgac aagggacgca gccctcgttc tcgtcatcga ctccctgacc tctctctact
    18841 ggacgtcctg gtcggacgcc gcgtgcgcgc ggcgactgac cctgtccatg tcaacggacc
    18901 gggccgcacc gacccagggc acggtaggaa cagaggtctc gtccgtcatc cgcggctcac
    18961 tgatcgtgac caaagggtga tcagggctga acccacggac acaagcacat tacctgtcag
    19021 taaccagcgc gccgcggcat catgtcactc gttcgggccc cgccaccgct gggctcccgt
    19081 cagggggccc gtctgcagcg gtcccggtcc gacatgtgtc gcggcgtcgg ctgcgggcgt
    19141 gctgaccacc agatccacgg gctggaacag cgggagcacc ggcagctgtg cccacagcac
    19201 cccttccacc acccctgcgg tggtcggccg cggcgcggcc gacagcagct cgtcgaacag
    19261 cggctgcagc gccgggaagc agaatcccgt cggtgagcgg ggcgggtcgg gcacgagagc
    19321 cgttgaggcg gggcacccgt agtcggaggc cagctccgtg ccgagatccc cgccgaccgt
    19381 gcgcggcagg atcatcatgt ccgcctcgac atctccgggc ggggccggtg gcgtcgtcgt
    19441 cggggacgcg gtcggagtcg cccggggcgc cgggggtgct acccggctcg ggcgcgcagg
    19501 gggtgctgcc gacgacggca ggggcgtcgg ggtgggagtc ggggtcgccg tcggcgtggg
    19561 cgggacggcc ggcaccacct gctgcgcaaa cagctgcgcg cccggcgggg cgatcaccgt
    19621 gacgccgacg cccgccgcct cgagctgcgc cgcgatcgtc tgggccacgc gcacgtcctc
    19681 gggccgctcc gccgcggcag ccagcacgag ctggaccggc gcgccgtcga cggcccagcg
    19741 cccggtggtc gggtcgcgcg tccagcccgc ggcggcgagc gcttgggccg ccgccaccgg
    19801 gttggggcgg agtggagcgt cgggaggcgc ggtggagccg tagccgggtt gggagggcgc
    19861 gagcccgaag gagtcggccg ggagtgccgc gggggccacg gcggcgcgca ccgcctcacg
    19921 gtcgacgagg agcgcgatgc cacggcgggc ccgcgggtcg gcgagcggac cggtgtctgc
    19981 gcgcatgccg agctgcgtga cgaccggctc cggcgccggc tgcagccggg gggcgggctg
    20041 cagccccccg agaagctgcc ggatcgccgg agtcgactcg ggtagggcca cgttcacctc
    20101 gccgctggcc agtccgtcgg cgagcacggg cccgtccatc cggcgcagca cgagctcatc
    20161 gagcaccgtc ggtgtgtccc agtagaggtc gttgcggtcc aggacgatct cgccccgggc
    20221 acggtcgacc gacgagatgc ggaagggccc gccggaggcg ggcaagccgc cggtgagcgc
    20281 tcccgtccac gatcccggcg cgtccttgag gattgccgcg ggcaacaggt cggagaacaa
    20341 ggtcttccac gctgggtagg cctgcgagaa cacgacgtcg acggccttgc ccccggcgcg
    20401 cgagcgcacg tccgtgatca gccggtagcc cgcggcgtcg gcgacaccgg gctggctgcg
    20461 catctgttcc cacaggtaga cgaagtcctc ggcggcgatc ggcgcgttcg acgaccagga
    20521 cgcctccagg ttgagctcgt agctcaccgt gaacggcgcg gtggagacca cctgcgcact
    20581 ggtcgcgacg gtggggtcga gctgcatgac gccgttcgcg tccgggtgga acaccgacgg
    20641 cagcacgagc gtcgccagtg ccgttgtcac cggcgagagg tcggcaagca ggtgcgggtt
    20701 gaagccggcg cccagctccc cgacaccgac gacgagccgc gtgggcgcgg gcggcggcgt
    20761 cggggcaggc gcgggcggcg ggggttggac gggcgcgttc gtgcaccccg ccagcagcac
    20821 ggcgagcagc agcaacagcg ctgcccccgt gcgtcgggcc agcggagcac gcatcggcac
    20881 cctgaacctc cacctgatcc ggcgggcact ccgcccacag gcggtcatcc tcgcaccgcc
    20941 gtcaggtgcc ccccatcggg cggggcggtc aggagaccga ccgcgcgcgc gagcgcatcc
    21001 gtgcccgggt gtgcgagtcg agctcgacct tccggatccg caccgactcg ggcgtgacct
    21061 ccacgcattc gtcgttggcg cagaactcca gcgcctgttc gaggttgagc accgtcggcg
    21121 gggtcagctt gaccagctcg tccgccgtgg acttgcgcat gttggtgagc ttcttctcgc
    21181 ggacgatgtt gacctcgagg tcctcgttgc gcgtgtactc cccgatgacc atgccgccgt
    21241 agacgatcgt gccggggccg acgaacagca cgccgcggtc ggcgagcgc
//
LOCUS       DASRSC010000129        24441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5119798,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000129 DASRSC010000000
VERSION     DASRSC010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 24441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24441
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5119798"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..774
                     /locus_tag="VFE39_06085"
     CDS             163..774
                     /locus_tag="VFE39_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6258204.1"
                     /translation="MPTVRPYRPDDRSALADICVRTGDAGEDARALYSDPDLLPSIFV
                     LPYVEFEPALAFVVDDDGLAVGYVVGTSDTPAFVEWFRREWLPGLAERYPRLVGRPVT
                     PTDSMIGLLHAPERMLVPEVAAYPAHLHIDLLPAYQRAGHGRGLMTQFLLALGVQGVA
                     AVHLGMLSANTRARAFYDRMGFHEIPVPAAPGTTYLGRATITS"
     gene            complement(800..2113)
                     /locus_tag="VFE39_06090"
     CDS             complement(800..2113)
                     /locus_tag="VFE39_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6258205.1"
                     /translation="MDPDRTPAVAAAEQESRPHQLRRVAFASAIGTTIEWYDYFIYST
                     AAALIFGSQFFSTLSPGSATLASFATLGVGFLARPVGGILWGHFGDRIGRKRMLVASL
                     VLMGVATVGVGVLPTYAQIGVAAPILLVTMRLLQGLSAGGEWGGAALMAVEHAPTGQR
                     GRYGAYSQIGVPAGLILAQLVFVVIGATLSDEQFASWGWRLPFLFSIVLVGVGLFIRL
                     RIEESPVFRALKTEQVRARLPMIDVLRERPRELLIASVSFIANTAIGYVFLAYLLSYG
                     TSVLGVSRTTMLLVVIVGSVSWLLSIIISARWSDRVGRKPVYVFGSVLLVVWPVPFFL
                     LIDTRSAALMVLAVIVLTIGLGASYGPQSALFAEMFEARYRYSGASFSYAVGAVLGGG
                     FAPLVATALQSSTGTSLSVSAYMVVTGLISLAAVLAIRETRRVSR"
     gene            complement(2179..2856)
                     /locus_tag="VFE39_06095"
     CDS             complement(2179..2856)
                     /locus_tag="VFE39_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6258206.1"
                     /translation="MIGVLLVDDQALVRAGFRALLGSQPDIEVLGEAGDGVAAVAAAH
                     ETRPDVVLMDIRMPGRNGLDATRDIVADPGLAGTRVVVLTTFAEDAYVFDAIRFGASG
                     FLVKDIEPVELINAIRVVHRGDALLSPVVTRRLIEEFAARAKAPQPARQVAALLEALT
                     EREREVVELVAAGLSNDEIAERLVVSRATAKTHVSRAMVKLGARDRAQVVVLAYESGL
                     VRPGWAG"
     gene            complement(2853..3974)
                     /locus_tag="VFE39_06100"
     CDS             complement(2853..3974)
                     /locus_tag="VFE39_06100"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6258207.1"
                     /translation="MLPTVVLPLAVAAFTLVGSHFAARSQPEARPLDALGIVLLLAGP
                     VALVVRRWHPPAVFAGIAAVLVAYFAAGYPFGPVFLAAFIALGTTVTTGHRVAAYAIT
                     GVAFPVLVAVYLIQHPAAGFPLTGASAWLAWLAALIALAEVWRARSERREQARTARAE
                     ADRRRGSEERLRIARDLHDVLGHHVSLINVQAGVALYLMDDDPEQARTALTAIKQSSR
                     DLLREMRSTLGVLRGVDEEPPQHPVAGLAALDDLVTATRTAGLPVTVEIEGTPRELPP
                     SVDSAAYRIVQEALTNTRRHAGPARASVLLTYTEDGLTMRIDDDGAGATGPTDGTGGN
                     GLPGMRERATALGGSLSPGPRPGGGFRVDAYLRTSGASR"
     gene            complement(4144..4398)
                     /locus_tag="VFE39_06105"
     CDS             complement(4144..4398)
                     /locus_tag="VFE39_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258208.1"
                     /translation="MPIEHSSPLTAPPGGVWTDDVGVITGALELRTTCSDDGSVQVSV
                     RYEEADEWYTVRGGACHVHDPADAEVLHTVLHGVLHRPEG"
     gene            complement(4401..5096)
                     /locus_tag="VFE39_06110"
     CDS             complement(4401..5096)
                     /locus_tag="VFE39_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005451807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease family protein"
                     /protein_id="HET6258209.1"
                     /translation="MAVGSPRAGNRDGSYWGVREQLVRMPVALLAALLLVIGVGGCTV
                     TAALPDARPVTSDEAHTQLAALVERAPGSMRGYSRARFPHWSTQGKGGCDTRDLALQR
                     DGTDIVVEPPCKIVSGVWNSPYDGATHTDPSEVDIDHVVPLAEAWRSGAADWDDDRRE
                     AFANDLDGPGLLTVTDNVNQAKGDQPPNLWKPKVTGYWCTYAELWIATKSTWDLSVTS
                     AERSALLDMLDRC"
     gene            complement(5177..8101)
                     /locus_tag="VFE39_06115"
     CDS             complement(5177..8101)
                     /locus_tag="VFE39_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6258210.1"
                     /translation="MTDFADALSRAIEGDVSFDDYTRHLYSRDASMYAITPRGVVAPR
                     HAGDVAAAVALAAEHDVAVLPRGAGTSLAGQTVGDALVLDFSRYMSTILDLDPEARTA
                     RVQPGVVQDQLNRAAGAHGLMFGPDTSTSNRATLGGMIGNNSAGSGSVRYGMTIDHVR
                     TLDVVLSDATTAHLAPVDQAERARRAEASTLEGRLYRDLPALVEEQRGAIASGFPGFW
                     RRSGGYRLDRLAAATETFDLATFVVGSEGTLVVATEATVGLVPKPKHTVIAVGHFRST
                     AAAIAATEDALSCDPAAVELMDRTILDLSRERIEYAALGEILQDDPDALLFVSFTGDD
                     AAELAGQLDRLVALWERHGHGYHTLRAETAAQQGALLKVRKSGLGLLMAASQGSNRPL
                     AFVEDTAVDPARLAEYTERFAKVLDRHGLRAGFYGHCSVGCLHIRPFVDLSRPDEVQR
                     MRAVAEEIRDLVTEFGGVNSSEHGDGLARSEFNRELFGDELYAAMRQVKGIFDPGNRL
                     NPGKIVDAPAMTDNLRDPALPPAPPLRTALDFTVVAGGQSGMRGAADRCMNIGLCRKS
                     DAGVMCPSYMATRREHDSTRGRANALVKALSAPDPRVGLTDAGLHDALDLCLMCKACK
                     SECPLSVDMATLKSEAMHQAHQVEGTPLRSRVFAGIRGLNRLGAATAPLSNLPGRVPA
                     LRALLGRTLGIAPKRPLPVFARRTLPRWFRKRNTVGGDRGPLTFLADSFTSFTEPAVG
                     MAAIELLERAGWDVRLEDSGCCGRAAFSKGLLDQAARQASGLVDALAGTSGPIAGVEP
                     SCLLTLRDEHRALLGDDPRVADVAGRARLVDDLLVEAIDSGALHLREDAWPAGRRILF
                     HGHCHQKAEVGTATTVALLRRIPGAEVVELDAGCCGMAGSFGFEAEHYDVSMQIGEDR
                     LFPAVRAEPDSTIVAATGVSCRQQIAHGTTRRAHHPVELIRAALAEPGTNAPPTNGTV
                     AR"
     gene            complement(8211..9380)
                     /locus_tag="VFE39_06120"
     CDS             complement(8211..9380)
                     /locus_tag="VFE39_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005162865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6258211.1"
                     /translation="MSPTGGRHFLQIPGPTNVPDRVLRAMSAPTIDHRGPEFAQLTQA
                     LLPALGPVFGTTGPVVIYPSSGTGAWEAALANTLSPGDRVLIGETGQFSTLWAELAGR
                     IGVQVEVIPGDWRHGVDPDAVGERLAADTEHAIKAVCVVHNETSTGVTSRVPDVRAAI
                     DTAGHPALLLVDTISSLASIDYRHDEWRVDVTVAGSQKGLMLPPGLGFNAVSEKALAA
                     SRSAGLPRSYWDWAPILEANARGFFPYTPATNLLYGLREALAMLAEEGLPAVFARHQR
                     HAAATRAAVRAWGLEVWCADEREHSGSLTAVLLPTGHDADEVRRIILERFDMSLGSGL
                     GRLAGTVFRIGHLGHLNDLTLAGTLSGVQMGLQLAGVPIKPGGIDAALDHLRDAG"
     gene            9614..11005
                     /locus_tag="VFE39_06125"
     CDS             9614..11005
                     /locus_tag="VFE39_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET6258212.1"
                     /translation="MSYQVISILALVAAFVLATMLPINLGAISLVAAFVIGTLALGKG
                     PDDVADAIFAGFPGSLFVILVGVTFLFAIARANGTVDWLVHMAMKGVGGRVALIPWVM
                     FLVTGVLTAFGAVVPAAVAIIAPVGMGFAVRHRINPVLMGLSIINGASAGGFSPLSIF
                     GVITNGVVAQNNLPGSPLTLFLGSLVFNVLLNTVVFLIFGGRELLGRVVDTTGETTDE
                     TAAASAPAGREPGTSGVASGGPGAARATTTGATGAGFTDGEHPPVTTLDRNRSLTLVA
                     LLGVAVGALAFQLDVGLLAITAAVLLSLLAPHTYKGAVAQVAWPTVLLICGIVTYVNL
                     MQDIGTVDFLGNQVAAIGVPLLAALVVCYIGAAVSAFASTTGILGALIPLAVPFLLAG
                     FVGPVGLITALAISSSVVDSSPFSTSGALVTANAPDDRRDYVYRRLMRWGFSMVAVAP
                     PVTWLLLVVPGWL"
     gene            11016..12242
                     /locus_tag="VFE39_06130"
     CDS             11016..12242
                     /locus_tag="VFE39_06130"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET6258213.1"
                     /translation="MQPQPAPEGPLAGITVLEVGVFMAGPFATMQLADLGARVIKVEN
                     PDAPDPTRATGPFVEGPDSRRHSSPFVRLNRNKESVALDLKSEAGREAFLRLAAGADA
                     LVENLRPGALRRLGLGPDELRGLNPGLVYASASGFGQDGPLAALPGLDIMAQARSGLM
                     SITGFPDGDPVKVGVPICDLVSGLYTALAVTAALLHRDQPHGDGRGQQVDVSLLESGV
                     SLAVWEAGTYFATGQVGSPLGSAHQTQAPYQALRCADGWVTVGAITPKTWDGLCAALD
                     LPHLHADPRYAGSSSRHAHRTELVAAIEDAIAPRAVAEVVAACEAAGVPCAPINDYGQ
                     VFTDAHLADRGFFWEAEHRDLGPVRQLGSPMRLSGTPARRGGAGPVLGADSRAVLGEV
                     GYTDAEIDALLRTMVG"
     gene            12313..13107
                     /locus_tag="VFE39_06135"
     CDS             12313..13107
                     /locus_tag="VFE39_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET6258214.1"
                     /translation="MSQELDGTQRDLLVERDGPVLRVTFDRPKAHNALTFAMYEGLYD
                     ACETADADDDVKVLVLRGAGGRAFVSGTDIAQFAGFDGSAGVAYEERISRVVRRLEDT
                     AVPTLAAVDGYCIGGGLAIAAACDLRIATAASRFGVPIARTLGNCLSMDTYALLLGHL
                     GPARTLDMLLRARLLSADDAHAAGFVTQLVDDAAGLDDALAETVDALASHAPLSMWAA
                     KESLTRLRRAGLPDGDDIVQRVYGSADFQAAVRSFAAKERPTWKGE"
     gene            13173..13943
                     /locus_tag="VFE39_06140"
     CDS             13173..13943
                     /locus_tag="VFE39_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET6258215.1"
                     /translation="MTQPDRSGGVQSLDRAFALLEHMADAGGDVALSELAVRSGLPLP
                     TIHRIVRTLVASGYVRQLPSRRYALGPRLIGLGDAASRMLGEWARPQLTQLVDQAGET
                     ANLAMLDGDAVVYVAQVPSRHSMRMFTEVGRRVPVHCTGVGKALLAQLPPSQVHPILV
                     RAGMPAQTPHTITDPERLARELDRIRHQGYAVDDAEQEVGVRCVAVAVPGGRSTAALS
                     VSGPETRVTWALVERTVPLLQQAAAALAGHLDGRGVTA"
     gene            13943..14521
                     /locus_tag="VFE39_06145"
     CDS             13943..14521
                     /locus_tag="VFE39_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6258216.1"
                     /translation="MNTEHLRLCASAEWARLVADDLLPWVLGEDRTSGNHVLGDDVLE
                     IGAGPGLVTDLLVERAPRVTAIEIDPELASGLRARMSGRPVEVITADATAMPMPDGRF
                     SAAACFTMLHHIPNAAGQDRALAEIARVLRPGGVLLGTDGQDTPARRALHVDDVFTPI
                     DPAMLLARLTAAGFAEAEVESNGDRFRFSARR"
     gene            14585..15613
                     /locus_tag="VFE39_06150"
     CDS             14585..15613
                     /locus_tag="VFE39_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6258217.1"
                     /translation="MTRRLAVVAQKVGVSEATVSRVLNGKPGVSEATRDAVLTALDVL
                     GYERPTRLRGERARLVGLVLPELQNPIFPAFAEVVGGALAQQGLTALLCTSSAGGVSE
                     AAYVDLLLQQQVSGAIFAGGQYTEADAQHDHYRLLLQRKLPTVFTNAAVDGLGFPQVS
                     CDDGEAVEQAFGHLVALSHQRIGLLLGPADHVPSQRKLAAFAAANARAGRAEHDKFVE
                     HAMFSLESGQASMLRLLDQGVTGVICASDPLALGAIRAARRRGLSVPGDLSVIGYDDS
                     ALMSCVDPALTTIRQPIEAMGRAAVELLVHQIEGSTVRTAELLYEPELVVRSSTAPFR
                     GDHVQKPT"
     gene            15694..17412
                     /locus_tag="VFE39_06155"
     CDS             15694..17412
                     /locus_tag="VFE39_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HET6258218.1"
                     /translation="MGGQASDELWWRDAVIYEIYPRSFADGDGDGVGDLPGVRSRLGY
                     LRELGVDAIWFTPWYLSPMADGGYDVTDYRAIDPAFGTLAEAEELITAALAVGIRTIV
                     DIVPNHVSDQHPWFQAALAAAPGSPERARFWFHPGRGPGGDEMPTRWPSMFGGDTWTR
                     TKNADGTPGEWYLHLFSTGQPDLNWSHPDVRREHQDVLRFWFDRGVAGVRIDSAALLV
                     KDPALPEIPAEPGPGEHPNTDRDELHEIYRGWREIADSYPADHTPGRRVLVGEIWLPD
                     VERFVRYLRPDELHTAFNFDFLARPWHAAQLRESIDATLAAHAPVGAPATWVLSNHDV
                     TRPVTRYGRAESGFSFAAKRAGTPTDPELGMRRARAAALLSAALPGSLYLYQGDELGL
                     PEYEQLDDELRQDPMYLRSAGLDPGREGCRVPLPWAGALPPFGFSPPGAQATWLPQPE
                     EWAVLTVAAQEAEPGSMLALYRAALRIRRSEPGLGDGPMTWLPSPDGVLAFARDQSFT
                     CVANLSERPVALPEHTAVLLSSAPLTDAGDLGTDTAAWLRTTPNIPDAATRGRRRRRS
                     DNEVHP"
     gene            17396..18769
                     /locus_tag="VFE39_06160"
     CDS             17396..18769
                     /locus_tag="VFE39_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6258219.1"
                     /translation="MRSIRKAATAVSAVLIAAGLLAACSASPGQPDAGGKTAITVVSL
                     KPGSEQSAFDAFENQVKQFEAANPDIDVTSQEYEWTGPTFTAQLAGGTLPTVFTVPFT
                     DGKSLIERGQLADLSQYVRAFGYADKFNPTVLRSGQSADGGIFGVPTAAYGVGLHYNR
                     TLFTQAGLDPDKPPTTWDEVRSYAKQIAERTGQAGYAQMTQENTGGWMLTSLTYALGG
                     RMQVANGDKVEATIDNPGAREALTRLREMRWQDNSMGSNFLYDWGSINQEFAAGRIGM
                     IMAGSDIYESLVQQNNIKPADYGLAVLPLAPGGSAGLLGGGTLAAVNVKASPKQQAAA
                     AKWIDFYYMSKFLKQDAAVLDAKTLSESKQPVGLPKLPIFDKATLDQYNQWIKPYVNV
                     PLAQMRSFNEGIFDQELAGEPTAHTQEMYAVLDTVVQAVLTDPGANIDELLSTADSDV
                     QAILDRG"
     gene            18772..19659
                     /locus_tag="VFE39_06165"
     CDS             18772..19659
                     /locus_tag="VFE39_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6258220.1"
                     /translation="MRTWVRRGGLSTLVFLLPLLVIFGVFSWLPIVRAAVMSVQQTNL
                     VTAPKFVGLENFQSVLADPLFWTAVRNTGVFALIALVFGYPIPLVVAVLMGEVRRFRG
                     LLSAIAYLPVVVPPVVAVLMWKFFYDARPDGVFNTILGWVGLGPYRWLQSATTAMPSL
                     VLEATWAAAGGTVIIYLAALIGVPAELYDAADVDGASIWQKIWHVTLPQLRGVLLVTL
                     ILQIIGTSQVFLEPFLFTGGGPANSTLTVLLLIYNYAFGSSLGGDYGAATALSLMLAV
                     FLAALSAVYFWLTRSWSTS"
     gene            19656..20657
                     /locus_tag="VFE39_06170"
     CDS             19656..20657
                     /locus_tag="VFE39_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6258221.1"
                     /translation="MSTPTSIRPVVPAGPQPVAVEPAPRQPARWSVRRARRRADDQPP
                     RGIVSDAERRTPSVRVALQLIHATLLALLLVIGLGPILWLVKASISTTQDTLRSPMAL
                     FPSGVDWANLATAWTRVHIDLHFANSMIIASGSWLVQLIVATTGGYALSILRPRYGPV
                     LYWLVLSTLFVPAVVLLVPLYLTVLDPPLLGVSLINTFWAVWLPAGANAFNVLLTKRF
                     FDNLPREIFDAARIDGAGPYRIFWSVLLPMSRPILGVVSVFAFIAAWKDFLWPLLVLP
                     NPDVQPLSVRLPTLARSTELDVLLAALMISALIPMVLFLVFQRTFLRSAGLSGALKG"
     gene            20686..21628
                     /locus_tag="VFE39_06175"
                     /pseudo
     CDS             20686..21628
                     /locus_tag="VFE39_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558180.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(21659..21940)
                     /locus_tag="VFE39_06180"
     CDS             complement(21659..21940)
                     /locus_tag="VFE39_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258222.1"
                     /translation="MSRAHRQPGSVGAPSTATVSVTCVSDGRAHAVPDSELAKGAAQP
                     EGYYSAVCGHIVAAAPMAAPDGEPCALCAEMWDLRNGRRQRDRSRRIFG"
     gene            22124..22363
                     /locus_tag="VFE39_06185"
     CDS             22124..22363
                     /locus_tag="VFE39_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF397 domain-containing protein"
                     /protein_id="HET6258223.1"
                     /translation="MHDNDGSEVAADQLRGVAWRKSQASNPNGDCVELAHLASGSVAV
                     RNSRDPRGPALVYTRAEIAAFIRGAKDGEFDDLIA"
     gene            22509..23411
                     /locus_tag="VFE39_06190"
     CDS             22509..23411
                     /locus_tag="VFE39_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6258224.1"
                     /translation="MRAGPDTWSPSGPPDPAQQSGPTVLRIVLGTQLRRLREASGITR
                     EAAGDAIRASHAKISRLELGRVGFKERDIVDLLALYGVTDAAEREEFLGLARRANARG
                     WWHQHGDILPSWFEMYLGLEQAASVIRTYQVQFVPGLLQSEEYARSVIRVGHQDESAD
                     EIDRRVQLRMTRQKMLTEPGAPQLWAVIDEAALSRPFGSPRVLREQLEHLLEMTSMSN
                     VTVQVLPFRFGIHAAAGGPFTILRFAESDLPDIVYLEQLNSAVYLDKRPEVEDYLAVM
                     ERVSVQAETPADTKALLREKLAET"
     gene            complement(23443..24279)
                     /locus_tag="VFE39_06195"
     CDS             complement(23443..24279)
                     /locus_tag="VFE39_06195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET6258225.1"
                     /translation="MAGRRHNTSSNRGLDGTVPPPEVDLNTPSIARSYDYILGGKEHF
                     EVDRRVTDAIYAAVPETGQIAKDNRNLLRRATRYLVGEAGIRQIIDLGSGLPTVGNVH
                     EIAHSVDPGVRVVYVDNDPIVLAHGRALLSEENTTTVITADVRDPGSIFDNPAVTEFI
                     DFDEPFAVMAVSILHHLTDDDAYATAEALKKQLTEGSYLLISNFLDDDEPRAKDLEKA
                     FLEGGLGSGRFRTWAELRRFYEGLEMLEPGLVYGNDWRPDRLTLTDSPVHTLYAAGMG
                     RK"
BASE COUNT         3493 a         8697 c         8628 g         3623 t
ORIGIN      
        1 ggcccacgcg ccgaacgccg tgcgcctcgc actcgctgcg ccaccggtgc cggtgctggc
       61 cgccgcgctc gacgtcctcg ccggcctggc caggggagca ccgggggacg acggggtgga
      121 gtgagcggac gggagtgatc acggtggtcg ttactgtgag acgtgcccac cgtgcgcccc
      181 taccggcccg acgaccgttc cgcgctggcc gacatctgcg ttcgcacggg cgacgccggt
      241 gaggacgccc gcgcgctgta ctccgacccc gacctgctgc cgagcatctt cgtcctgccc
      301 tacgtcgagt tcgagcctgc gctcgcgttc gtcgtcgacg acgacggcct ggccgtcggc
      361 tatgtggtgg gcacctcgga cacgcccgcg ttcgtcgagt ggttcaggcg ggagtggcta
      421 cccgggctcg ccgagcgcta cccccggctg gtgggccgac cggtcacgcc gaccgactcg
      481 atgatcgggc tgctgcacgc ccccgagcgg atgctcgtgc ccgaggtggc ggcctacccg
      541 gcccacctgc acatcgacct gctgcccgcc taccagcggg cgggccacgg gcggggattg
      601 atgacgcagt tcctgctggc gctgggcgtg cagggggtgg cggcggtgca cctgggcatg
      661 ctcagcgcca acacccgcgc acgagccttc tacgaccgga tggggttcca cgagatcccg
      721 gtgcccgccg cgcccggcac gacctacctc ggtcgcgcga cgatcacgtc gtgagggtgc
      781 gcacgagcac gccgcgcggc taacgcgata cccgccgtgt ctcccggatg gccagcaccg
      841 cggcgaggct gatcaggccc gtcaccacca tgtacgccga cactgacaac gacgtgccgg
      901 tggagctctg cagcgcggtg gcgaccagcg gcgcgaagcc gccaccgagc acggcaccga
      961 cggcgtagga gaacgaggcg ccgctgtagc ggtagcgcgc ctcgaacatc tccgcgaaca
     1021 gcgccgactg cggaccgtag gacgcaccga gcccgatcgt caggacgatc acggcgagca
     1081 ccatcagggc cgccgagcgc gtgtcgatca gcaggaagaa cggcaccggc cacaccacca
     1141 gcagcaccga cccgaagacg tagaccggct tgcggcccac ccggtccgac cagcgcgccg
     1201 agatgatgat gctcagcagc cacgagacac tgccgacgat cacgaccagc agcatcgtcg
     1261 tgcggctcac gccgagcacg gaggtgccgt acgagagcag gtaggccagg aagacgtagc
     1321 cgatcgcggt gttggcgatg aagctgaccg aggcgatgag cagctcccgc ggccgctcgc
     1381 gcagcacgtc gatcatcggg aggcgggcgc gcacctgctc ggtcttcagc gccctgaaca
     1441 ccgggctctc ctcgatccgc aggcggatga acaggccgac gccgacgagc acgatgctga
     1501 acaggaacgg caaccgccag ccccagctcg cgaactgctc gtcgctgagc gtcgccccga
     1561 tgaccacaaa caccagctgg gcgaggatca gcccggccgg caccccgatc tgcgagtagg
     1621 ccccgtaccg gccgcgctgc ccggtcggcg cgtgctcgac ggccatcagt gcggcaccgc
     1681 cccactcgcc gccggcgctg agcccctgca gcaggcgcat cgtgaccaac aggatcggcg
     1741 cggcgacacc gatctgcgcg taggtcggca gcaccccgac cccgaccgtc gcgacgccca
     1801 tgagcaccag cgacgcgacg agcatgcgct tgcggccgat ccggtcgccg aagtggcccc
     1861 acaggatccc acccacgggc cgggcgagga acccgacgcc cagcgtcgcg aaggatgcca
     1921 gggtggccga gccaggcgag agggtcgaga agaactgcga gccgaagatc agcgccgcgg
     1981 cggtggagta gatgaagtag tcgtaccact cgatcgtcgt gccgatcgcg ctggcgaacg
     2041 ccacccgtcg tagctgatgg gggcgcgact cctgctcggc cgcggccacc gctggtgtcc
     2101 tgtccgggtc cacccgccac cccctgtccg tcgtcggaca ccttgggagg agaggtgcgc
     2161 agatgtcaac cgatctcctc agcctgccca gccggggcgc acgagcccgc tctcgtacgc
     2221 cagcacgacg acctgggcgc gatcccgtgc cccgagtttg accatggccc ggctgacgtg
     2281 ggtcttcgcg gtggctctgc tcaccacgag ccgttccgcg atctcgtcgt tcgacagccc
     2341 ggctgccacg agttccacca cctcccgctc acgctcggtg agggcctcga gtagcgcggc
     2401 gacctgccgc gcgggctgcg gtgccttggc ccgtgctgcg aactcctcga tcagccgtcg
     2461 ggtgacgacg ggcgagagca gcgcgtcgcc gcggtgcacg acccggatcg cgttgatgag
     2521 ctcgaccggc tcgatgtcct tgaccaggaa cccgctggcg ccgaaccgga tggcgtcgaa
     2581 cacgtaggcg tcctcggcga acgtcgtgag gacgaccacg cgggtgccgg cgaggccggg
     2641 gtcggcgacg atgtcgcggg tggcgtcgag gccgttgcgg ccgggcatgc ggatgtccat
     2701 cagcacgacg tcggggcggg tctcgtgggc cgcagccacg gcggcgacgc cgtcccccgc
     2761 ctcgccgagc acctcgatgt cgggctgcga accgagcagc gcgcggaacc cggcccggac
     2821 gagggcctgg tcgtcgacga gcaggacccc gatcaccggc tcgccccgga ggtgcgcagg
     2881 taggcgtcca cgcggaagcc gccgccgggg cgcggacccg gggagaggga cccgccgagc
     2941 gccgtggcgc gttcccgcat gccgggcagt ccgttgccgc ccgtcccgtc ggtcggtccc
     3001 gtcgctccgg cgccgtcgtc gtcgatccgc atcgtgagac cgtcctcggt gtaggtcagg
     3061 agcaccgacg ccctcgcggg cccggcgtgg cggcgcgtgt tcgtcagcgc ctcctgcacg
     3121 atccggtatg ccgccgagtc gacgctcggc ggcagctcgc gcggcgtccc ctcgatctcg
     3181 acggtcaccg gtagcccggc ggtacgggtg gccgtgacga ggtcgtccag cgcggcgagc
     3241 ccggccaccg ggtgctgcgg gggttcctcg tcgacgccgc gcagtacgcc gagggtggag
     3301 cgcatctcgc gcaggaggtc ccggctcgac tgcttgatcg cggtgagtgc ggtgcgggcc
     3361 tgctcgggat cgtcgtccat caggtacagc gccacgccgg cctggacgtt gatcagcgag
     3421 acgtggtggc cgagcacgtc gtgcaggtcg cgggcgattc ggaggcgttc ctcgctaccg
     3481 cgcctgcggt cggcctcggc ccgtgctgtc ctcgcctgct ccctgcgctc gctgcgcgcc
     3541 cgccacacct cggccagggc gatcagcgcc gcgagccatg ccagccaggc ggaggcaccg
     3601 gtcagcggga agcccgctgc gggatgctgg atcaggtata ccgccacgag cacggggaac
     3661 gcgacgccgg tgatcgcgta ggcggccacc cggtgcccgg tcgtgacggt ggtcccgagt
     3721 gcgatgaacg cggccaggaa caccgggccg aacgggtacc ccgcagcgaa gtaggcgacc
     3781 agcacggcgg cgatgcccgc gaacacggcg ggcgggtgcc accggcgcac cacgagggcg
     3841 accggaccgg ccaggagcag cacgatcccg agtgcgtcga ggggtcgggc ctcgggctgg
     3901 cttcgtgcgg cgaaatggct gccgaccagc gtgaacgcgg ccacggcgag cggcaggacc
     3961 acggtgggca gcacaccggc ccaccccgtc ggaccccgcc acgtcggatc ccgccacgta
     4021 ggatcccgcc acgtaggacg ctgcggcgga tgcacccgcc cacggtagcc agcgttcgcg
     4081 ctccccacgt ccgtctgcgg gagtgcgccg gcctaccgcg cccggcgtac ggggtgggcc
     4141 aactcagccc tccggccggt gcagcacgcc gtgaaggact gtgtgcagga cctcggcgtc
     4201 ggccgggtcg tgcacgtggc acgctccgcc gcgcacggtg taccactcgt ccgcctcctc
     4261 gtagcggacg ctgacctgca cggaaccgtc gtcgctgcac gtggtgcgca gctccagggc
     4321 tccggtgatc acgccgacgt cgtcggtcca cacgccgccg ggcggcgcgg tgagtgggga
     4381 gctgtgctcg atgggcatcg tcagcacctg tccagcatgt cgaggagcgc ggagcgttcg
     4441 gcggaggtta ccgaaaggtc ccatgtggac ttagtggcga tccagagttc tgcgtaggtg
     4501 caccagtacc cggtcacctt gggcttccac aggttcggcg gctggtcgcc cttggcctgg
     4561 ttgacgttgt ccgtgacggt gagcagtccg gggccgtcca ggtcgttcgc gaacgcttcg
     4621 cgccggtcgt catcccagtc cgcggccccg gagcgccacg cctcggccag cggcaccacg
     4681 tggtcgatgt cgacctcgga cgggtccgtg tgggtcgccc cgtcgtaggg cgagttccag
     4741 acgccgctga cgatcttgca ggggggctcg acgacgatgt ccgtgccgtc gcgctgcagg
     4801 gccaggtcgc gggtatcgca gccacccttg ccctgggtgg accagtgcgg aaaccgggcc
     4861 cgggagtaac cgcgcatcga tccgggcgcg cgttcgacga gcgcagcaag ctgggtgtgc
     4921 gcctcgtccg acgtgacggg ccgggcgtcc ggaagcgcag ccgtgaccgt gcagccgccg
     4981 acgccgatga cgagcagcag cgccgcgagg agcgccaccg gcatccgcac gagctgttcg
     5041 cgcacgcccc agtagctccc atcccggttg ccggcacggg gcgaacccac ggccacgacg
     5101 cgacgatcat gccatcagtc ccgggacagt ctgatcacag cccgatcgac cggacgaacg
     5161 gcgctctcgt gctggcctat cgggcgacgg tgccgttcgt cgggggcgcg ttcgtgccgg
     5221 gctcggcgag ggcagcccgg atcagctcga cggggtggtg ggcccggcgg gtggtgccgt
     5281 gcgcgatctg ctggcggcac gagaccccgg tggcggcgac gatcgtggag tcgggctcgg
     5341 cgcgcaccgc cgggaagagg cggtcctcac cgatctgcat ggagacgtcg tagtgctcag
     5401 cctcgaagcc gaacgagccc gccatcccgc agcacccggc gtcgagctcg acgacctcgg
     5461 cgcccgggat ccgccgcagc agcgcgaccg tggtggccgt gcccacctcg gccttctggt
     5521 ggcagtgccc gtggaagagg atgcgccgcc ccgcgggcca ggcatcctcc cgcaggtgca
     5581 gggctccgct gtcgatcgcc tcgacgagca ggtcgtcgac gagccgggcg cgaccggcca
     5641 cgtccgccac gcgcgggtcg tcgcccagca gtgcgcggtg ctcgtcgcgc agcgtcagca
     5701 ggcaggacgg ctcgacgccc gcgatcggcc cggacgtgcc ggcgagcgcg tcgaccagcc
     5761 cggacgcctg gcgcgcggcc tggtcgagca ggcccttcga gaacgccgcc cgtccgcagc
     5821 agccgctgtc ctccaggcgc acgtcccagc cggcgcgctc cagcagctcg atcgcggcca
     5881 tgccgaccgc gggctcggtg aagctggtga acgagtcggc gaggaacgtc agtggacccc
     5941 ggtccccacc gacggtgttc cgcttgcgga accaccgcgg cagcgtacgg cgcgcgaaca
     6001 ccggcagcgg gcgtttcggc gcgatcccga gggtgcgtcc gagcagcgcg cgcagcgcgg
     6061 ggacccggcc gggcagattc gacagcggcg cggtggccgc gccgagccgg ttcagcccgc
     6121 ggatgccggc gaagacccgg gagcgcaggg gtgtgccctc tacctggtgg gcctggtgca
     6181 tcgcctcgct cttcagcgtc gccatgtcca ccgacagcgg gcactcgctc ttgcatgcct
     6241 tgcacatcag gcacaggtcg agggcgtcgt gcagtccggc gtcggtgagc ccgacccgag
     6301 ggtcaggtgc ggacagcgcc ttgaccaggg cgttcgcccg cccgcgcgtg gagtcgtgct
     6361 cgcgccgcgt cgccatgtag gacgggcaca tcacgccggc gtcgctcttg cggcacaggc
     6421 cgatgttcat gcaacggtcg gcggcgccac gcatcccgct ctggccgccg gccaccacgg
     6481 tgaagtccag cgcggtgcgc agcggtgggg ccgggggcag cgccgggtcg cgcaggttgt
     6541 cggtcatcgc cggagcgtcg acgatcttgc ccgggttcag ccggtttcca gggtcgaaga
     6601 tccccttcac ctggcgcatc gccgcgtaga gctcgtcgcc gaacagttcc cggttgaact
     6661 cgctccgggc gagcccgtcg ccgtgctcag aggagttgac gccgccgaac tcggtgacga
     6721 ggtcgcggat ctcctcggcc accgcgcgca tccgctgtac ttcgtcggga cgggacaggt
     6781 cgacgaacgg gcggatgtgc aggcagccca cggagcagtg tccgtagaac ccggcccgca
     6841 gcccgtgccg gtcgagcacc ttcgcgaagc gctccgtgta ctcggcgagc cgggccgggt
     6901 caaccgcggt gtcctcgacg aacgcgagcg gccggttcga gccctggctc gcggccatga
     6961 gcagcccgag gcccgacttg cggaccttga gcagtgcgcc ctgctgcgcg gcggtctcgg
     7021 cgcgcagcgt gtggtagccg tggccgtggc gctcccagag cgcgacgagc cggtcgagct
     7081 ggccggcgag ctctgccgcg tcgtcaccgg tgaaggagac gaagagcagc gcgtcggggt
     7141 cgtcctgcag gatctcgccc agcgccgcgt actcgatccg ttcccgggag agatcgagga
     7201 tcgtgcggtc catcagctcg acggccgccg ggtcgcagct cagcgcgtcc tccgtggcgg
     7261 cgatcgccgc ggcggtggag cggaagtggc ccaccgcgat cacggtgtgc ttcggcttgg
     7321 gcaccaggcc cacggtggcc tcggtggcca ccacgagcgt gccttccgag ccgaccacga
     7381 acgtcgcaag gtcgaaggtc tccgtggcgg cggccagccg gtccagacgg tacccgccgg
     7441 agcgacgcca gaagccgggg aacccgctgg cgatcgcgcc acgctgctcc tcgaccagcg
     7501 ccggcaggtc ccggtagagc cgtccctcca gcgtggaggc ctcggcgcgg cgcgcccgct
     7561 ccgcctggtc gacgggggcg aggtgcgcgg tggtggcgtc ggagagcacc acgtcgagcg
     7621 tgcggacgtg gtcgatcgtc atgccatagc gcacagaccc gctgcccgcg gagttgttgc
     7681 cgatcatccc gccgagcgtg gcgcggttgg acgtggacgt gtccggcccg aacatcagcc
     7741 cgtgcgctcc ggcagcccgg ttgagctggt cctgcaccac ccctggctgc acacgcgcgg
     7801 tgcgggcctc cgggtcgagg tcgaggatcg tgctcatgta ccgggagaag tcgagcacca
     7861 gcgcgtcgcc gacggtctgt ccggccaggc tggtgcccgc gccacggggc agcacggcga
     7921 cgtcgtgctc ggcggcgagc gccaccgcgg cggccacgtc accggcgtgg cgcggcgcca
     7981 ccaccccacg cggcgtgatc gcgtacatgc tggcgtcgcg cgagtagagg tggcgggtgt
     8041 agtcgtcgaa ggagacgtcc ccctcgatcg cgcgggagag tgcgtcggcg aaatcggtca
     8101 ccgctgctcg ccgcccctgg ctcgccccgc gaggcgtcga tcaaggcgca gcggtcgctc
     8161 ggtcggcggt gcgggcgacc tcaaggcctt gatctgcggg atctgagcgc tcatccggcg
     8221 tcccgcaggt ggtccagcgc cgcgtcgatg ccgccgggct tgatcggcac cccggcgagc
     8281 tgcagcccca tctggacacc ggagagcgtg ccggccagcg tcaggtcgtt gaggtggccg
     8341 aggtgcccga tccggaacac cgtgccggcg agccggccga gcccggagcc cagcgacatg
     8401 tcgaaccgtt cgaggatgat ccggcgcacc tcgtcggcat cgtgaccggt gggcagcagc
     8461 accgccgtca acgacccgga gtgctcccgc tcgtcggcgc accacacctc caacccccac
     8521 gcccgcaccg cggcgcgggt ggccgcggcg tggcgctgat gccgggcgaa gacggcgggc
     8581 aggccctcct cggcgagcat cgcgagcgcc tcccgcagcc cgtagagcag gttggtggcc
     8641 ggggtgtagg ggaagaaacc gcgggcgttc gcctccagga tcggcgccca gtcccagtag
     8701 gaccggggca gccccgccga gcgcgaggcc gccaacgcct tctcgctcac cgcgttgaag
     8761 cccaaccccg gcggcaacat caggcccttc tgcgagcccg cgaccgtgac gtcgacccgc
     8821 cactcgtcgt gccggtagtc gatggaggcc agcgaggaga tcgtgtccac cagcagcagg
     8881 gcggggtgcc cggcggtgtc gatcgcggcc cggacatcgg gtacccggct ggtgaccccg
     8941 gtggaggtct cgttgtgcac cacgcacacg gccttgatcg cgtgttcggt gtcggcggcc
     9001 agccgctcgc cgaccgcgtc ggggtccacg ccgtggcgcc agtcgcccgg gatcacctcg
     9061 acctgcacac cgatccgccc ggccagctcc gcccacaacg tcgagaactg cccggtctcc
     9121 ccgatcagca cccggtcacc cgggctgagc gtgttggcca gcgccgcctc ccacgcccct
     9181 gtgccagacg acgggtagat caccaccggt cccgtggtgc cgaacaccgg gcccagcgcg
     9241 ggcagcaacg cctgcgtcaa ctgagcgaac tccgggccgc ggtggtcgat cgtgggcgcc
     9301 gacatcgccc gcagcacccg gtccggcacg ttcgtcggcc ccgggatctg caggaagtga
     9361 cgccctccgg tgggactcat gtgatgcctc caggtgcgtc gtcattgtcg cttcgtggaa
     9421 gacatctacc gcatcccgaa cggacgcaac agggtcgtac acggaaacga ggtgcgcgac
     9481 actcggtgtc cacgccgggc cttgaccgta tccataccga gccttgacct agtcgcgtga
     9541 ccagaaccat actcctgtac ggaatccgct ttccgcataa cggaacaacg ccgtccggcc
     9601 atgaggaagt gccatgtcct accaggtgat ctcgatcctt gcgctggtgg cggcgttcgt
     9661 cctcgccacc atgctcccca tcaacctcgg cgccatctcg ctcgtcgccg cattcgtcat
     9721 cgggaccctg gcgctcggca agggcccgga tgacgtcgca gacgcgatct tcgccggctt
     9781 ccccggcagc ctgttcgtga tcctggtcgg ggtcacgttc ctgttcgcca tcgcccgcgc
     9841 aaacggcacg gtcgactggc tggtgcacat ggccatgaag ggcgtgggcg gccgggtcgc
     9901 cctgatcccg tgggtcatgt tcctggtcac cggcgtgctc acggcgttcg gtgccgtggt
     9961 gcccgcggcg gtcgcgatca tcgccccggt ggggatgggc ttcgcggtcc gccaccggat
    10021 caacccggtg ctgatgggtc tctcgatcat caacggagcc agtgcaggcg ggttctcgcc
    10081 gctcagcatc ttcggtgtga tcaccaacgg ggtggttgcc cagaacaacc tgcccggcag
    10141 cccgttgacc ctcttcctcg gcagcctcgt gttcaacgtg ctgctcaaca ccgtcgtctt
    10201 cctgatcttc ggcgggcgcg agctgctggg gcgcgtcgtt gacaccacgg gcgaaaccac
    10261 ggacgagacg gccgcggcga gcgcaccggc cgggcgcgag ccgggcacca gcggagtcgc
    10321 atccggcggt ccgggcgccg cgcgggcgac caccacgggc gccacgggcg ccggcttcac
    10381 ggacggcgag caccctcccg tcaccacact cgaccgcaac cgctcgctga cgctcgtcgc
    10441 gctgctcggc gtggccgtcg gggccctggc cttccaactg gacgtgggcc tgctcgcgat
    10501 caccgcagcg gtcctgctct cgctgctcgc cccccacacc tacaagggcg cggtcgccca
    10561 ggtggcctgg ccgaccgtgc tgctgatctg cgggatcgtc acctacgtca acctcatgca
    10621 ggacatcggc accgtcgact tcctgggtaa ccaggtcgcc gcgatcggcg tgccgctgct
    10681 cgccgcgctg gtggtctgct acatcggcgc cgccgtgtcg gcgttcgcgt ccaccaccgg
    10741 catcctcggt gcgctgatcc cgctggcggt gccgttcctc ctcgccgggt tcgtgggccc
    10801 ggtcggtctc atcaccgcgc tggcgatctc ctcgtcggtg gtcgactcca gcccgttctc
    10861 cacctccggc gccctcgtca cggccaacgc cccggacgac cgccgcgact acgtctaccg
    10921 ccgcttgatg cggtggggct tctcgatggt cgcggtcgcg cctcccgtca cgtggctcct
    10981 gctggtggtg ccgggttggc tgtgaccatg tgagcatgca gccacagcca gcacccgaag
    11041 gccccctcgc cgggatcacg gtgctcgagg tcggagtctt catggcgggc ccgttcgcca
    11101 ccatgcagct cgccgacctc ggggccaggg tcatcaaggt cgagaacccc gacgcgccgg
    11161 atcccacccg cgccaccggg ccgttcgtcg agggtcccga ctcccggcgg cacagctcgc
    11221 cgttcgtccg gctcaaccgc aacaaggagt cggtggcgct ggacctgaag tccgaggccg
    11281 gccgggaggc gttcctccgg cttgccgccg gcgccgatgc gctggtggag aacctccggc
    11341 ccggggcgct gcggcgcctc gggctcgggc ccgacgagct cagggggctc aaccctgggc
    11401 tcgtgtacgc ctccgcgtcc ggcttcgggc aggacgggcc gctggcggcg ctgcccggac
    11461 tggacatcat ggcccaggcg cgctccgggc tgatgagcat caccggcttc cctgacggcg
    11521 atccggtcaa ggtgggcgtg ccgatctgcg acctcgtcag cgggctctac acggcgctcg
    11581 cggtcaccgc cgcgctgctg caccgggacc aaccgcacgg cgacggccgg gggcagcagg
    11641 tcgacgtgtc gctgctggag tcgggcgtgt cgctcgcggt gtgggaggcc gggacatact
    11701 tcgccaccgg gcaggtcggc agcccgctcg gctctgcgca ccagacacag gcgccgtacc
    11761 aggcgctgcg ctgcgcggac ggctgggtga ctgtcggggc gatcaccccg aagacctggg
    11821 acggcctctg cgccgcgctg gacctgccgc acctgcacgc cgacccccgc tacgccggct
    11881 cgagcagccg ccacgcccac cgcaccgagt tggtcgccgc gatcgaggac gccatcgcgc
    11941 cgcgggccgt cgcggaggtc gtcgcggcgt gcgaggccgc gggcgtcccg tgcgcgccga
    12001 tcaacgacta cggccaggtg ttcaccgacg cccacctcgc cgaccggggc ttcttctggg
    12061 aggccgagca tcgcgacctc ggcccggtcc gccagctcgg ctcaccgatg cgactgtcgg
    12121 gaacaccggc ccgccggggc ggtgccggcc cggtgctggg ggccgactcg cgtgccgtgc
    12181 tcggtgaggt cgggtacacc gacgcggaga tcgacgcgtt gctcaggacg atggtcggat
    12241 gagagagcgt gcgagcgaat cagtgtcaca gcgccttcgc gtagcgccgg ccgggcgtag
    12301 cgagggagtg cgatgagcca ggaactagac ggaacacagc gggatctcct cgtcgagcgc
    12361 gacggtcccg tcctgcgtgt cacgttcgac cggccgaagg cgcacaacgc actgacgttc
    12421 gcgatgtacg agggcctgta cgacgcctgc gagaccgccg acgccgatga cgacgtgaag
    12481 gtgctcgtcc tgcgcggggc cggcggcagg gcgttcgtct ccggcacgga catcgcccag
    12541 ttcgcgggtt tcgacggctc cgcaggcgtg gcctacgagg agcggatcag ccgggtcgtg
    12601 cgcaggctgg aggacacggc ggtgccaacc cttgcggccg tcgacggcta ctgcatcggc
    12661 ggtggcctcg ccattgccgc cgcctgcgat ctccgcatcg ccaccgcggc gtcgcggttc
    12721 ggggtgccga tcgcgcgtac gctgggcaac tgcttgtcga tggacaccta cgcccttctg
    12781 ctgggacacc tcggccccgc tcgcacgctc gacatgctgc tgcgggcccg cctgctcagc
    12841 gccgacgacg cccacgccgc gggattcgtc acccagctcg tggacgacgc cgcgggcctc
    12901 gacgatgcgc tcgccgagac cgtcgacgcg ctggcgtcac acgctccgct gagcatgtgg
    12961 gcggccaagg agtcgctcac ccggctgcgc cgggccgggc tccccgacgg ggacgacatc
    13021 gtccagcggg tgtacggcag tgccgacttc caggccgccg tccggtcctt cgcggccaag
    13081 gagcgcccca cgtggaaggg cgagtgaggc cggccgacta gcctgcgagg tccgcaacct
    13141 caccggaacc gaaccggaga accatgggtg gtgtgacgca acccgaccgc agtggcggcg
    13201 tgcagtcctt ggaccgggcg ttcgccctgc tcgagcacat ggccgacgcg ggtggcgacg
    13261 tcgcgctctc ggagctcgcc gtccggtccg gtctgccgtt gccgacgatc caccggatcg
    13321 tccgcacgct cgtggcgtcg gggtacgtcc ggcagttgcc gtcgcgccgc tacgccctgg
    13381 gtccccggtt gatcggcctc ggtgacgccg cgtcccggat gctgggggag tgggccaggc
    13441 cgcagctcac ccagctggtc gaccaggccg gcgagaccgc caacctcgcg atgctcgatg
    13501 gcgacgccgt ggtctacgtg gcgcaggtcc cgtcgcggca ctccatgcgc atgttcaccg
    13561 aggtcgggcg acgggtgccg gtgcactgca cgggggtggg caaggcgctg ctggcccagc
    13621 tgccgccatc gcaggtacat cccatcctgg tgcgggcggg catgcccgcg cagaccccgc
    13681 acaccatcac cgaccctgag cggttggcca gggagctgga ccggatccgg caccagggct
    13741 acgcggtcga cgacgccgag caggaggttg gcgtgcgctg cgtggccgtc gccgttcccg
    13801 gcggacggtc cacggcggcg ctgtccgtat ccgggccgga gacgcgggtc acctgggccc
    13861 tggtggagcg caccgtgccg ctgctgcagc aggcggccgc ggcgctcgcc gggcacctcg
    13921 acgggcgagg agtgaccgcg tagtgaacac ggagcacctg cggctgtgcg cgagcgccga
    13981 gtgggcccgg ctcgtcgcgg acgatctgtt gccctgggtg ctcggcgagg accggacgtc
    14041 cggcaaccac gtgctcggtg acgacgtact ggagatcggg gccggccccg gactggttac
    14101 ggacctgctc gtggagcggg ccccgcgcgt caccgcgatc gagatcgacc ccgagctcgc
    14161 gtcggggctg cgtgcgcgga tgagcggccg gcccgtcgag gtgatcaccg cggacgcgac
    14221 ggcgatgccg atgcccgatg gccggttctc cgccgcggcg tgcttcacga tgctgcacca
    14281 catcccgaac gccgccggtc aggaccgcgc gctggccgag atcgccagag tcctgcgtcc
    14341 cggcggggtg ctgctcggca ccgacggcca ggacaccccg gcccgccggg ccctgcacgt
    14401 cgacgatgtc ttcaccccga tcgacccggc catgctgctc gcccggctca ccgcggccgg
    14461 gttcgccgag gccgaggtgg agagcaacgg cgaccggttc cggttcagcg cgcgccgctg
    14521 atcggtgccg taagcgcgcg gaagcgctgc aagaacttgc aagaacctgc gctacagttg
    14581 cgccatgacg cgacggctgg cggtggtggc gcagaaggtc ggggtcagtg aggccacggt
    14641 cagccgggtg ctcaacggga agccgggcgt ctccgaggcg acgcgtgacg ccgttctcac
    14701 cgcgctcgac gtgctcggct acgagcggcc cacccgcctg cgcggggagc gcgcccggct
    14761 ggtcgggctt gtcctccccg agctgcagaa cccgatcttt ccggcgttcg ccgaggtggt
    14821 cggcggcgca ctggcgcagc agggcctcac ggcgctgctg tgcaccagct ccgcgggcgg
    14881 ggtcagcgag gcggcctacg tcgacctgct gctccagcag caggtgtcgg gcgcgatctt
    14941 cgccggcggg cagtacaccg aggccgatgc ccagcacgac cactaccgcc tgctgctgca
    15001 gcgcaagctg cccacagtgt tcaccaacgc ggccgtcgac ggtctgggct tcccccaggt
    15061 ctcgtgtgac gacggggagg ccgtcgagca ggcgttcggc cacctggtgg ccctcagcca
    15121 ccagcgcatc gggctgctcc tgggcccggc cgaccacgtg ccgtcgcagc gcaagctggc
    15181 cgcgttcgcc gccgcgaacg cgcgggccgg acgcgccgag cacgacaagt tcgtcgaaca
    15241 cgccatgttc tcgctggaga gcggccaggc gtccatgctg cggctgctcg accagggggt
    15301 cacgggcgtc atctgcgcga gcgacccgct cgcgctcggg gcgatccggg ccgcgcgccg
    15361 gcgtggcctg tcggtgcccg gcgacctgtc ggtgatcggc tacgacgact cagccctgat
    15421 gagctgcgtc gatcctgcgc tcaccacgat ccggcagccg atcgaggcga tgggccgcgc
    15481 ggcggtcgag ctgctggtgc accagatcga gggcagcacg gtgcgcacgg cggagctgct
    15541 gtacgagccg gagctcgtgg tgcggtcctc gaccgctccc ttcagaggag atcatgttca
    15601 gaaaccgact tgatatagtc gaactcttgc gttgatctgc tattgatcgg taccgtcctc
    15661 gccacacgat cggatgacga cgggcggggt ggcgtgggcg ggcaggcatc ggacgagctg
    15721 tggtggcgcg atgcggtgat ctacgagatc tacccccgca gcttcgccga cggtgacggc
    15781 gacggcgtag gcgatctgcc gggggtccgg tcccgtctcg gctacctgcg tgagctcgga
    15841 gtcgacgcca tctggttcac tccctggtac ctctcgccga tggccgacgg cggctacgac
    15901 gtcaccgact accgtgcgat cgaccccgcg ttcggcaccc tcgccgaggc ggaggagctg
    15961 atcacggccg cgctggccgt cggcatccga acgatcgtcg acatcgtccc gaaccacgtc
    16021 tccgaccagc acccctggtt ccaggccgcg ctggccgcgg cgccggggtc acccgagcgg
    16081 gcccggttct ggttccatcc cgggcgcggc cccggcggcg acgagatgcc gacgcgctgg
    16141 ccgtcgatgt tcggcggcga cacctggacc cgcaccaaga acgccgacgg cacgccgggt
    16201 gagtggtacc tgcacctgtt cagcacaggg cagccggacc tcaactggag ccaccccgac
    16261 gtccgccgcg agcaccagga cgtgctgcgg ttctggttcg accgcggcgt cgccggcgtg
    16321 cggatcgact cagcggcgct gctggtcaag gaccccgcgc tgccggagat cccggccgaa
    16381 cccggccctg gcgagcaccc caacaccgac cgcgacgagc tgcacgagat ctaccgcggc
    16441 tggcgcgaga tcgccgactc gtacccagcc gaccacaccc cgggccggcg ggtcctggtc
    16501 ggcgagatct ggctgcccga cgtcgagcgc ttcgtccgct acctgcggcc cgacgagttg
    16561 cacacggcgt tcaacttcga cttcctggcc cggccgtggc acgcggcgca gctgcgcgag
    16621 tcgatcgacg ccacgctggc cgcccacgcg ccggtgggtg cgcccgccac ctgggtgctg
    16681 tccaaccacg acgtcacgcg gccggtcacg cgctacggcc gcgccgagtc cgggttctcc
    16741 ttcgcggcca agcgggcggg caccccgacc gacccggagc tgggcatgag gcgggccagg
    16801 gcggccgcgc tcctgtcggc ggcgctgccc ggctctctgt acctctacca gggcgacgag
    16861 ctcgggctgc ccgagtacga gcagctcgac gacgagctcc ggcaggaccc gatgtacctg
    16921 cgctccgcag gcctggaccc gggccgggaa ggctgccggg tgccactgcc gtgggccggt
    16981 gccctcccac cgttcgggtt ctccccgccc ggggcgcagg ccacgtggct gccgcaaccc
    17041 gaagagtggg cggtgctgac cgtcgcggcg caggaggccg aacccggctc gatgctcgcg
    17101 ctctaccgcg ccgcgctgcg gatccgccgc tccgagcccg gcctcgggga cggcccgatg
    17161 acctggttgc cctccccgga cggcgtgctg gcgttcgctc gcgatcagtc cttcacctgt
    17221 gtcgcgaacc tgtcagagcg gccggtggcg ctccccgagc acaccgccgt gctcctgtcg
    17281 agcgccccgc tcaccgacgc aggcgacctg ggcaccgaca cggcggcctg gctgcgcacg
    17341 accccgaaca tcccggacgc cgcaactcgc ggtcggcgaa ggagaaggag cgacaatgag
    17401 gtccatccgt aaggccgcga ccgcggtatc ggcagtgctg atcgctgccg gcctcctggc
    17461 cgcatgcagc gcctcgccag gtcagccgga tgcgggtgga aagaccgcca tcacggtggt
    17521 cagcctcaag cccggtagcg agcagtccgc gttcgacgca ttcgagaacc aggtcaagca
    17581 gttcgaggcg gcgaaccccg acatcgacgt gaccagccag gagtacgagt ggacgggtcc
    17641 gacgttcacc gcgcagctcg ccgggggaac cctgcccacc gtcttcaccg tgcccttcac
    17701 cgacggtaag tctctgatcg agcgcggcca gttggccgac ctgagccagt acgtccgggc
    17761 attcggctac gccgacaagt tcaacccgac cgtgctgcgg tccgggcaga gcgccgacgg
    17821 cgggatcttc ggggtgccga cggcggccta cggcgtcggc ctgcactaca accgcacgct
    17881 cttcacgcag gccgggctcg acccggacaa gcctccgacc acgtgggacg aggtgcgcag
    17941 ctacgccaag cagatcgccg agcgcaccgg gcaggccggc tacgcacaga tgacgcagga
    18001 gaacaccggc ggctggatgt tgacctcgct cacctacgcc ctcggcggcc ggatgcaggt
    18061 cgccaacggc gacaaggtgg aggccacgat cgacaatccc ggcgcgcggg aggcgctcac
    18121 gcggctccgg gagatgcgct ggcaggacaa ctcgatgggc agcaacttcc tctacgactg
    18181 gggaagcatc aaccaggagt tcgccgccgg ccggatcggc atgatcatgg ccgggtcgga
    18241 catctacgag tcgctggtgc agcagaacaa catcaagccg gccgactacg ggctcgcggt
    18301 gctgccgctc gccccgggcg gctcagcagg cttgctcggc ggcggcacgc tggccgcggt
    18361 gaacgtcaag gcctcgccga agcagcaggc cgccgcggcg aagtggatcg acttctacta
    18421 catgtccaag ttcctgaagc aggacgccgc ggtgctcgac gccaagacac tgtcggagag
    18481 caagcagccg gtcggcctgc cgaagctgcc gatcttcgac aaggcgaccc ttgaccagta
    18541 caaccagtgg atcaagccgt acgtcaacgt gccgcttgcc cagatgcgct ccttcaacga
    18601 gggaatcttc gaccaggagc tcgctggtga gcccaccgcg cacacccagg agatgtacgc
    18661 ggtactcgac acggtcgtgc aggctgtgct cacggacccc ggggcgaata tcgacgagct
    18721 cctgagcaca gccgactcgg acgtccaggc gatcctcgac cgaggctgag catgcgaacc
    18781 tgggtccggc gcggcggcct gagcacgctg gtcttcctcc tgccactgct ggtgatcttc
    18841 ggcgtgttct cctggctgcc gatcgtccgg gcggcggtca tgagcgtgca gcagaccaac
    18901 ctggtgactg cgccgaagtt cgtcgggctg gagaacttcc agtcggtgct ggccgatcca
    18961 ctgttctgga ccgcggtgcg caacaccggc gtgttcgcgc tcatcgcact ggtgttcggc
    19021 tacccgatcc cgctcgtggt ggccgtgctg atgggcgagg tgcgccggtt ccgcgggctg
    19081 ctgagcgcca tcgcctacct gccggtcgtg gtgccgccgg tggtggcggt gctcatgtgg
    19141 aagttcttct acgacgcccg tcccgacggg gtgttcaaca ccatcctcgg ctgggtcggg
    19201 ctgggcccgt accggtggct gcagtcggcc accaccgcga tgccgtccct cgtgctggag
    19261 gccacctggg cggcagcggg gggcacggtg atcatctacc tcgccgcact gatcggggtg
    19321 cccgcggagc tgtacgacgc cgccgacgtg gacggcgcgt cgatctggca gaagatctgg
    19381 cacgtcacgc tgccgcagct gcgcggggtg ctgctggtca ccctgatcct gcagatcatc
    19441 ggcacctccc aggttttcct cgagccgttc ctgttcaccg ggggcggccc ggccaactcg
    19501 acgctcacgg tgctgctgct gatctacaac tacgcgttcg gctccagcct tggcggcgac
    19561 tacggggcgg cgacggcctt gagcctcatg ctcgcggtgt tcctggcagc gctgtccgcc
    19621 gtctacttct ggctcacccg atcctggagc acctcatgag cacgccgacc agcatccgac
    19681 ccgtggtccc cgccgggccg cagccggtcg ccgtggagcc cgctccccgg cagcccgccc
    19741 ggtggtccgt tcggcgggcc cgccgacggg ccgatgacca gccgccgcgc gggatcgtct
    19801 cggacgcgga gcggcgcacg ccgtccgtgc gggtggcgct gcagctcatc cacgccacgt
    19861 tgctggcgct gctgctggtg atcgggctcg ggcccatcct gtggctggtc aaggcctcga
    19921 tcagcaccac ccaggacacc ctccgcagcc cgatggcgct gttcccgagt ggggtggact
    19981 gggcgaacct ggccaccgca tggacaaggg tgcacatcga cctgcacttc gccaacagca
    20041 tgatcatcgc ctcgggctcc tggctcgtgc agctgatcgt cgccaccacc ggcggctacg
    20101 cgctctcgat cctgcggccc cggtacgggc cggtgctgta ctggctggtg ctcagcacgc
    20161 tgttcgtccc cgcggtcgtg ctgctcgtgc cgctctacct gaccgtgctc gacccaccgc
    20221 tcctgggggt ctcgctgatc aacaccttct gggcggtctg gctaccggcc ggagcgaacg
    20281 ccttcaacgt cctgctcacg aaacggttct tcgacaacct gccgcgggag atcttcgacg
    20341 cggccaggat cgacggggcg ggtccgtacc ggatcttctg gtcggtgctg ctgccgatgt
    20401 cgcgaccgat cctcggcgtg gtgtcggtgt tcgccttcat cgcggcctgg aaggacttcc
    20461 tctggccact gctcgtgctg cccaacccgg acgtccaacc gctctccgtg cggctgccca
    20521 ccttggcccg gtcgaccgag ctggacgtcc tgctcgcggc actgatgatc tcggcgctga
    20581 tcccgatggt gctgttcctg gtgttccagc ggacgttcct gcgcagtgcg ggtctgagcg
    20641 gagcgctcaa gggttagagc gtgtcccgcg gatcttgctc ggtggatgcg gtacacctcg
    20701 ttcgtgcttg atcgtcatga cctgaccgat gaggaatggg cccggctgga gccacttctg
    20761 cccgatcaga ccccgcgccg gggtggccgg tgggccgatc accgcatggt gatcaacggt
    20821 gtgttctggc ggacccggtg cgggctgccc tggcgcgacg tgccgccggt ctacggacac
    20881 tggaagacgg tctataaccg gcatcgccgc gggtcaggcg accagacgtg ggtgcggatc
    20941 cttgatgagc tacgtcgcga cgccgacatg gccgagggcg cggagtggat ggtcggggtt
    21001 gacgccgggg tggtccgtgc tcaccagcac gccgccggtg cccgccacca gttccccgcc
    21061 gatgttgccg aggaggtcgt cgcacccctg gagctgcaca ccgggggcag ggtcggatga
    21121 caagaaatcg gtggccgagc ccagccggga gtcgctcggt cggtcccggg gcgggctgac
    21181 gtccaagatt cacctcgccg cggacagtcg ttgtcgtctg atctcgcggg tcaccaccgc
    21241 cggtcaccgg cacgactcgc tggcgttcga gccggtgatg gtcgggatcg cgattcgccg
    21301 ccgtggaccc ggccggcctc ggacccgacc cgggcgggtg ctgggtgaca tggcctactc
    21361 cagccgggcg atccgctccc atctgcgacg acgccggatc accgcgacca tcccggagcc
    21421 gactgatcag cggaataacc gactgcgccg tggccgcagc ggcggtcgac cacccgggtt
    21481 cgacccggag ggctacaagc agcgcaacgt cgtcgagcgt gcgatcaaca agctcaaggg
    21541 tcaccgtgcg gtggccaccc gctacgacaa gcgcgactac atgttccgcg gaaccgtcga
    21601 tgtcgcctcg atcaggatct ggctacgcga ccctgtccca tgtttcaagg gacacgctct
    21661 agccgaagat gcggcgggac cggtcgcgct ggcggcgccc attgcggagg tcccacatct
    21721 ctgcgcagag cgcgcagggc tctccatccg gagccgccat cggagctgcc gcgacgatgt
    21781 ggccgcagac ggccgagtag tagccctcgg gctgtgcggc gcccttggcg agctcggagt
    21841 cgggcacggc gtgggctcgg ccgtcgctga cacaggtgac ggacaccgtc gccgtcgacg
    21901 gcgcgccaac cgatcccggc tgccgatgtg ctctgctcac ggctccccct ccgcacacag
    21961 tcgtctgccg ggtgtgcccg aaagcacacc gcccggtgga tcagtctgcc gtctaccgct
    22021 tgcatccgca agggtgcctg caacagcatc tgcaattgca tctgcaagcc acactccttc
    22081 gggatggcac gtcccgaggg tccggaccgg tggaggatcg gacatgcatg acaacgacgg
    22141 cagcgaggtc gccgctgatc aactccgcgg cgtcgcctgg cggaagagtc aggcgagcaa
    22201 ccccaacggt gactgcgtcg agctggccca cctcgccagc ggctcggtcg cggtccgcaa
    22261 ctcgcgggac ccacggggac ctgcgctcgt ctacacgcgt gcggagatcg cggcgttcat
    22321 ccgaggtgca aaggacggcg agttcgacga cctcatcgcc tgagcgaacc ggacgccgac
    22381 cacgacggac cgttggggtc tatgatcctc ggcctggagg tggcagtgtt gccgaccggc
    22441 tgctatttcg cccgcttttc cggggcccga ccgagttgtc aggacaggtt cgtcgtgaag
    22501 gttgagctgt gagggcaggg ccggacacct ggtcgccgtc gggtccgccc gatcctgccc
    22561 agcagagtgg gcccaccgtc ctccgcatcg ttctcggtac ccagctccgg cggttgcggg
    22621 aggccagcgg gatcacccgc gaggcggcag gagacgcgat acgggcttcg cacgccaaga
    22681 tcagccgcct ggagctgggt cgggtgggct tcaaggagcg ggacatcgtc gacctgctcg
    22741 cgctgtacgg cgtcaccgac gccgcggagc gcgaggagtt cctcggtctg gccaggcggg
    22801 cgaacgcgcg gggctggtgg caccagcacg gggacatcct gccgagctgg ttcgagatgt
    22861 acctcgggct cgagcaggcg gcgagcgtca tccgcaccta ccaggtccag ttcgtgcccg
    22921 gcctgctgca gagtgaggag tacgcccgca gcgttatccg ggtcggccac caggacgagt
    22981 ccgccgacga gatcgaccgg cgggtacagc tgcggatgac ccgccagaag atgctcacgg
    23041 agcccggggc accccagttg tgggccgtga tcgacgaggc ggcgctgagc cggccgttcg
    23101 gctccccgag ggtcttgcgc gagcagttgg agcacctgct cgagatgacc tccatgtcga
    23161 acgtcaccgt ccaggtgctc ccgttccgct tcggcatcca tgccgccgcg ggcggcccct
    23221 tcacgatcct ccggttcgcc gagtccgacc tgcccgacat cgtctacctc gagcagctca
    23281 acagcgccgt ctacctggac aagcgtcctg aggtcgagga ctacctcgcc gtcatggaac
    23341 gggtgagcgt ccaggccgag acgccggccg acacgaaggc cctcctgcgg gagaagctcg
    23401 cggaaacgtg agggtgctcg gcagcccgag gtgcggctca ggtcacttcc ggcccatacc
    23461 ggccgcatag agcgtgtgca ccgggctgtc ggtgagggtg agcctgtcgg ggcgccagtc
    23521 gttgccgtag accaggcccg gttcgagcat ctcgagccct tcgtagaagc ggcggagctc
    23581 ggcccacgtg cggaagcgcc ccgaccccag gccgccctcc aggaacgcct tctcgagatc
    23641 cttcgcccgt ggctcgtcgt cgtccaggaa gttgctgatc agtaggtagg agccctcggt
    23701 gagctgcttc ttgagcgcct ccgccgtggc gtaggcgtcg tcgtcggtga ggtggtgcag
    23761 gatgctcacg gccatgacgg cgaacggttc gtcgaagtcg atgaactccg tgaccgcggg
    23821 gttgtcgaag atcgagcccg ggtcgcggac gtcggcggtg atcacagtgg tcgtgttctc
    23881 ttcgctgaga agcgctcggc cgtgggcgag gacgatcggg tcattgtcga cgtagaccac
    23941 gcgtacgccg gggtcgacgc tgtgcgcgat ctcgtgcacg ttgcccacgg tgggcaggcc
    24001 cgagccgaga tcgatgatct gacgtatccc ggcctcgccc acgaggtacc gcgtcgcccg
    24061 gcgcagcagg ttgcggttgt ccttcgcgat ctggcccgtc tccgggaccg ccgcatatat
    24121 cgcgtcggtt acccggcgat cgacctcgaa gtgctccttg cccccgagaa tgtagtcgta
    24181 ggaccgggcg atcgacggtg tgttcagatc cacttccgga ggcggcaccg tgccgtccag
    24241 gccccggttg ctggaggtgt tgtgtctccg gccagccatc acattctcct ccaacggcga
    24301 catcgaaggg cacccgaggc cgctcccgaa gatcgtgggc gcaagagtag cgctagacgg
    24361 tgatgagctc ggtgccgtgc agcccgagat cgtcgagctg ggcccgtacc tcctgctggt
    24421 agatcgggtt catgaggagc a
//
LOCUS       DASRSC010000130         7878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3319850,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000130 DASRSC010000000
VERSION     DASRSC010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3319850"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..623
                     /locus_tag="VFE39_06200"
     CDS             <1..623
                     /locus_tag="VFE39_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6258226.1"
                     /translation="GWTGVLVVNGLVRQFDSAVVLAGVGFTLDAGRAAAVVGPNGAGK
                     TTLLRCVVGADRPDAGEVLLDGRPLREDDPRARAMVASVLDDVEFFPDLSVAEHLDLL
                     ARAHGAEPDTAPVLAELGLAALADRTPVGLSSGERRRLALASCLVRPRRLLVLDEPEQ
                     RLDAAGRQWLVERLELEKAAGCAVLFASHDAAVVDAVADQTVDLGT"
     gene            620..2011
                     /locus_tag="VFE39_06205"
     CDS             620..2011
                     /locus_tag="VFE39_06205"
                     /inference="COORDINATES: protein motif:HMM:NF039718.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6297 family protein"
                     /protein_id="HET6258227.1"
                     /translation="MTTAPPVQVPPARELRTHLARVRRDRRAAVREGPGPDWYTPLLA
                     VAVLGGLLVQGMRRVMGAESPRLPDAPHTGLLLGAVGLVLTGMVLRAALALGPMMAGP
                     AVRHWVLAGTVDRAAMLRPRYVALSAVAALAGAGGGALAALVAAVAPPWWAALWWAGA
                     GSGSAAALAAAAVVWQAGNARAGRAVGTALVAAGGAAAVAALLVPRASAVPALPAAAV
                     ATVAWLLAVLLLACGWRRLGVLDRAALGAGSGLAAAVQVAGTFMEPSLLLGLLEERRW
                     RSVHRGRSRPGRGTGYGALLYADLRRVLRTPGALPLAVGLLLVTYAAAAVLPAVVVPP
                     VAAVAAYAVAGRVATGLRAVTRSPALRRALGGADRALIVVHLVAPLAVVIVWTVALLP
                     VLHLLNPIAVVLIPVGAVVVVERSATRPPPDYGAAVFDTGFGGMQVDLVRQVVRGPAL
                     LVGLAAVQLLLAR"
     gene            2112..2336
                     /locus_tag="VFE39_06210"
     CDS             2112..2336
                     /locus_tag="VFE39_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258228.1"
                     /translation="MNSSVVLTDFGAVVGVALLLVMALAPFFTDRHRPLSESPLKQAV
                     DTRVVIPVQRGARLRVPPATRPARPHAAPR"
     gene            2421..4286
                     /gene="dnaG"
                     /locus_tag="VFE39_06215"
     CDS             2421..4286
                     /gene="dnaG"
                     /locus_tag="VFE39_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET6258229.1"
                     /translation="MAGRIRDSDIAEVRDRTRVDEVIGEYVALRRAGAGSLKGLCPFH
                     DEKSPSFNVRPSHGTFHCFGCGEGGDAVAFIMKIEVVGFVEAVERLADRVGYRLTYEG
                     GGSSVQRDRGTRSRLLEANKRAAEFYAAQLRTPEAAPAIQFLTSRGFDSAAAQQFGCG
                     YAPGGWDLLTKHLLAGGFSLDELMKAGLSKEGRRGAIDRFHRRLLWPIRDLGGDVVGF
                     GARRLFDDDGIEAKYLNTSDTPVYRKTHVLFGLDLAKREIAKRRQVVVVEGYTDVMAM
                     HLAGVPTAVASCGTAFGTEHISVIRRLIGDDSFDLGEVIYTFDGDAAGQAAALKAFEG
                     DQNFAAQTFVAIAPDGQDPCELRQAHGDTAVRDLVARREPLFEFAIRSMLRDHDLDTA
                     EGRVAALQRCVPLVARIRREDLRDEYARRLAGWTSWDDIAMVVRRVRESAGAPAEPVR
                     GRIGNRREAPSPPPKDDPRLHLQREALKAALQVPVIAGPSYDELPEQAFTHPALAAVH
                     RAVQAAGGASGGRDGHAWLEAVLAACNSETRSLVTELAVEPLELPRRNTEEARYVSSV
                     IAGVRLALVEAQVAELKARLQRTNPMEEADAYHQLFGDLVPLEQYRIALREKAAR"
     gene            4377..4904
                     /locus_tag="VFE39_06220"
     CDS             4377..4904
                     /locus_tag="VFE39_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258230.1"
                     /translation="MNLLQAVRKLLGRDQLPEGFTGHLDVDEQVLATARLGGGGHVVA
                     TSLGLWLPTPDGVRRLSWHLVSKASWENGAIVLVEAQERDVVGGAVLIADLPPQRLRL
                     AEPGRVPEMVHARVTGSIRSRHHRDLPGGGAWFVQRKVPGRDGIVLQVRADPGTDDVA
                     VRQVAGGVARTLGLG"
     gene            4909..5679
                     /locus_tag="VFE39_06225"
     CDS             4909..5679
                     /locus_tag="VFE39_06225"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HET6258231.1"
                     /translation="MTGSTTWDPALYLDFDDHRSRPFHDLLARVGAVAPRRVVDLGCG
                     PGHLTAVLSARWPEAEVVALDSSAEMVAAARERGIDAEQADVVDWMPPPDTDVVVTNA
                     VLQWVPPHRQLLPRWIEALPGGAWFALQVPGNLGAPSHALIRELAEEELWRGRVDLRG
                     EIAVAEPAEYAELLSGAGAAVDVWETTYQQRLTGPDPVLQWISGTALRPVRDALGADA
                     YAAFREQLAPRLRAAYPARPDGTTWFPFRRIFAVARTP"
     gene            5927..6784
                     /locus_tag="VFE39_06230"
                     /pseudo
     CDS             5927..6784
                     /locus_tag="VFE39_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
     gene            6885..7250
                     /locus_tag="VFE39_06235"
     CDS             6885..7250
                     /locus_tag="VFE39_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HET6258232.1"
                     /translation="MSTGLGLSNVITITIFAVLLAIVVSETTSNTASAAVVVPIVIPI
                     AVAAGVNPFVPALAATFAASFGFMLPVSTPQNAIVYGSGVVPITTMIRSGAAFDLIGA
                     ILIIVFLPLMVAVLGLGVA"
     gene            7247..>7878
                     /locus_tag="VFE39_06240"
     CDS             7247..>7878
                     /locus_tag="VFE39_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HET6258233.1"
                     /translation="MTPQTIAVIPGDGIGSEVVDAARTVLDAVCARHDIELHYTEFDW
                     SCRRFEREGAMMPPDGIDTLRGFDAILLGAVGWPGVPDHVSLWGLLIPIRRAFRQYVN
                     LRPIRVFDGVQSPLRGAEDVDFVVVRENVEGEYSEVGGRANRGFPDEMAVQESIFTRV
                     GVSRIADFAFELAQTRRGYLTSATKSNGIVHTLPFWDEVVAERAARYPDVR"
BASE COUNT          943 a         2739 c         2988 g         1208 t
ORIGIN      
        1 gggggtggac gggcgtgctg gtcgtgaacg gcctggtcag gcagttcgat tccgcagtgg
       61 tgctggccgg tgtcggcttc accctcgacg caggtcgggc ggccgctgtg gtcggaccca
      121 acggcgcggg caagaccacc ctcctgcgct gtgtcgtcgg cgcggaccgg cccgatgcag
      181 gcgaggtgct cctcgacggc cgcccgttgc gtgaggacga tccgcgggcc cgcgcgatgg
      241 tcgcgagcgt gctcgacgac gtcgagttct tcccggacct ctcggtggcg gagcacctcg
      301 acctgctcgc gcgggcccac ggcgccgagc ccgacaccgc gccggtgctc gccgagctgg
      361 gcctggcggc gttggccgac cgcacgccgg tcggcctctc cagcggggag cgcagacgcc
      421 tggccctggc gtcctgcctc gtacgcccgc ggcggctgct cgtgctcgac gagccggagc
      481 agcggctcga cgcggcgggc cggcagtggc tggtggagcg gctcgaactg gagaaggcgg
      541 ccggctgcgc cgtgctgttc gcctcccacg acgcggccgt cgtcgacgcg gtggccgacc
      601 agacggtcga cctgggcacg tgaccaccgc cccacccgtg caggtgccgc ccgcgcgaga
      661 gctgcgcacc cacctcgctc gcgtccggcg ggaccggcgc gccgcggtca gggagggccc
      721 tgggcccgac tggtacaccc cgctgctcgc cgtggccgtg ctcggcgggt tgctggtgca
      781 ggggatgcgc cgggtgatgg gcgcggagtc gccccggctg ccggacgccc cgcacaccgg
      841 gttgctgctc ggcgccgtcg ggctggtgct gacgggcatg gtgctgcgcg cggcgctggc
      901 tctcggcccg atgatggccg gaccggcggt gcggcactgg gtgctcgccg gcaccgtcga
      961 ccgcgccgcc atgctcaggc cccgttacgt cgctctgtcg gcggtggcgg cgctggccgg
     1021 ggccggcggt ggggcgctcg cggccctggt cgccgcggtg gccccgccct ggtgggcggc
     1081 gctgtggtgg gccggggcgg gctcgggttc cgccgccgcg ctcgcggccg cggccgtggt
     1141 gtggcaggcg ggcaacgcgc gcgccggccg ggccgtgggc accgccctcg tggcggccgg
     1201 cggtgcggct gctgtcgccg cgctgctggt tccccgcgcg tccgccgtgc cggccctgcc
     1261 cgccgcggcg gtggccaccg tcgcatggct gctcgccgtg ctgctgctcg cctgcgggtg
     1321 gcggcggctc ggtgttctgg accgcgcggc tctcggggcg gggagcgggc tcgccgccgc
     1381 cgtccaggtt gccgggacgt tcatggagcc gtcgctgctg ctcggtctgc tggaggagcg
     1441 gcgctggcgc tcggtccacc ggggacgctc gcggcctggc cgcggcaccg ggtacggcgc
     1501 gctgctgtac gccgacctgc gacgcgtgct gcgcacacct ggcgcgctgc cgctcgcggt
     1561 gggcctgctg ctcgtgacct acgcggctgc cgccgtgctg cccgccgtgg ttgtgccacc
     1621 tgtcgccgcc gtcgccgcct acgcggtggc cggccgggtg gcgacgggat tgcgcgccgt
     1681 tacccgctcc ccggcgctcc ggcgagcgct ggggggcgcc gaccgggcac tgatcgtcgt
     1741 ccacctcgtc gcgcccctcg ccgtcgtcat cgtgtggacc gtggcgctgc tccccgtatt
     1801 gcacctgctg aacccgattg cggtggtact gatccccgtg ggggcggtcg tggtggtgga
     1861 gcgctcggcc acccggccgc ccccggacta cggcgccgcc gtgttcgata ccgggttcgg
     1921 tggcatgcag gtggacctgg tgcggcaggt ggtgcgcggg cctgcgctgc tggtggggct
     1981 cgcggcagtc cagctcctgc tggcccgctg acgggtcgga cgcgggcatt ggccggggtt
     2041 taacccgatt cggggggtat tcgcaccctc agccaaggcg cgtctcaggt tccgcggcgc
     2101 acggtggaga catgaactca tccgtcgttc tcacggactt cggggccgtc gtgggagtcg
     2161 ctctgctgct ggtcatggcc cttgcgccgt tcttcaccga tcggcaccga cccctctccg
     2221 agagtcccct gaagcaggcc gtggacaccc gcgtggtgat ccccgtccag cgcggggccc
     2281 gcctccgtgt gccgcccgcc actcgcccgg caaggccgca cgcggcacct cggtgatcgg
     2341 ggcaacggcg acccgacccg cggaggaacg ccgatcgcgg cgctcgtagg tccgtccgat
     2401 gtcgggccta cactcggccc gtggcagggc ggatccggga cagcgacatc gcggaggtcc
     2461 gcgaccggac ccgagtcgac gaggtcatcg gcgagtacgt ggctctgcgc cgcgccggcg
     2521 ccggatcgct caagggcctc tgcccgttcc acgacgagaa gtcgccctcg ttcaacgtcc
     2581 gcccatcgca cggcacgttc cactgcttcg gctgcggcga gggcggtgac gccgtcgcgt
     2641 tcatcatgaa gatcgaggtc gtcgggttcg tggaggccgt ggagcgcctc gccgaccggg
     2701 tgggctaccg gctgacgtac gagggaggcg gcagctccgt acagcgcgac cgcggtaccc
     2761 ggtcgcggct gctcgaggcc aacaagcgtg ccgcggagtt ctacgccgcc cagctgcgca
     2821 cccccgaggc cgcgccggcc atccagttcc tcaccagccg cgggttcgac tccgcagcgg
     2881 cgcagcagtt cggctgcggc tacgccccgg gcggctggga cctgctcacc aagcacctgc
     2941 tcgcgggcgg gttctccctc gacgagctga tgaaggccgg cctgtcgaag gagggccgcc
     3001 gcggcgcgat cgaccgcttt caccgccgcc tgctctggcc gatccgcgac ctcggtggcg
     3061 acgtcgtcgg cttcggggcc cgccgcctct tcgacgacga cggcatcgag gccaagtacc
     3121 tcaacacgtc cgacacgccg gtgtaccgga agacccacgt cctgttcggg ctcgacctcg
     3181 ccaagcgtga gatcgccaag cgccgccagg tcgtggtcgt cgagggctac accgatgtca
     3241 tggcgatgca cctggcgggt gtccccaccg cggtcgcgtc ctgtggcacg gcgttcggca
     3301 ccgagcacat ctcggtgatc cgtcgcctga tcggcgacga ctcgttcgac ctcggcgaag
     3361 tgatctacac cttcgacggc gacgcggccg ggcaggctgc ggcgctcaag gcgttcgagg
     3421 gcgaccagaa cttcgccgcg cagacgttcg tggcgatcgc gcccgacggg caggatccct
     3481 gcgagctgcg gcaggcccac ggcgacacgg ccgtacgcga cctggtggcc cgccgtgagc
     3541 cgctgttcga gttcgccatc cggagcatgc tgcgcgacca cgacctcgat acggccgagg
     3601 gtcgggtggc cgcgctgcag cgctgcgtgc cgctcgttgc gcggatccgt cgggaggatc
     3661 tgcgcgacga gtacgcccgc aggctagcgg gctggaccag ctgggacgac atcgcgatgg
     3721 ttgtgcggcg ggtccgggag tcggcgggcg cccctgccga gcccgtcagg ggacgcatcg
     3781 ggaacaggcg cgaggccccg agcccgcctc cgaaggacga cccgcggctg cacttgcagc
     3841 gggaggcgct caaggccgcc ctgcaggtac cggtcatcgc cggcccgagc tacgacgagc
     3901 tgcccgagca ggccttcacc cacccggcgc tggcggcggt gcaccgcgcg gtgcaggccg
     3961 cgggtggggc gtccggcggg cgcgacggtc acgcgtggct cgaggccgtg ctcgccgcgt
     4021 gcaactcgga gacgcgatcc ctggtcaccg agctcgcggt ggagcccctg gagctgccac
     4081 ggaggaacac ggaggaggcg cgctacgtct cgagtgtcat cgccggggtg cggctcgcac
     4141 tggtggaggc ccaggtggcg gagttgaagg cacggctgca gcgcaccaac ccgatggaag
     4201 aggccgacgc ctaccaccag ctgttcggcg atctcgtgcc gctggagcag taccggatcg
     4261 ccctgcggga gaaggcggcg cggtgagcgg cgagggcgtg cggtgagtga gcgtgcgagt
     4321 gaaccatcgg cgcagcgcct gggttcgttg ccgaccgagc gcagcgaggg agagcgatga
     4381 acctcctgca ggcggtgcgc aagctgctcg gacgcgacca gctgcccgag ggctttaccg
     4441 gccacctcga cgttgacgag caggtgctcg ccacggcacg gctgggcggc ggcgggcacg
     4501 tggtcgccac ctcactcggg ctgtggctgc ccacgcccga cggcgtgcgg aggctgagct
     4561 ggcatctggt gagcaaggcg tcgtgggaga acggcgcgat cgtgctggtc gaggcccagg
     4621 agcgcgacgt cgtcggcggt gcggtgctga tcgccgacct gcccccgcag cggctgcggc
     4681 tcgccgagcc cggccgggtg ccggagatgg tgcacgcgcg cgtcacgggc tcgatcagga
     4741 gcaggcacca ccgggacctg cccgggggcg gtgcgtggtt cgtccagcgc aaggtgccgg
     4801 ggcgcgacgg aatcgtgctg caggtgcgtg ccgacccggg caccgacgac gtcgcggtcc
     4861 ggcaggtagc cggcggcgtc gcgcgcacgc tggggttggg ctgacgaggt gacgggcagc
     4921 accacctggg atccggcgct gtacctcgac ttcgacgacc accgctcccg cccgttccac
     4981 gacctgctcg cgcgggtggg cgcggtcgcg ccgcgccgtg tggtcgacct gggctgcggt
     5041 ccgggacact tgacggccgt gttgagcgcg cgctggccgg aggccgaggt cgtcgccctg
     5101 gactcgtctg cggagatggt ggctgccgcg agagagcgcg ggatcgacgc ggagcaggcc
     5161 gacgtcgtcg attggatgcc gccgcccgac accgacgtgg tggtcaccaa cgcggtgctg
     5221 cagtgggtgc caccgcaccg gcagctgttg ccgcggtgga tcgaggccct accgggcggc
     5281 gcgtggttcg cgctgcaggt gccgggcaac ctcggcgccc cgtcccacgc gttgatccgg
     5341 gagctggccg aggaggagct gtggcgcggc cgggtcgacc tgcgcggtga gatcgccgtg
     5401 gccgaaccgg cggagtacgc cgagctgctg tcgggcgccg gcgcggcggt cgacgtctgg
     5461 gagacgacct accagcagcg gctcacgggg ccggatccgg tgctccagtg gatcagcggc
     5521 acagcgctgc ggccggtgcg tgacgcgctc ggcgccgacg cgtatgccgc cttccgtgag
     5581 cagctggccc cgcggctgcg tgcggcgtac ccggcccggc cggacggcac cacgtggttc
     5641 ccgttccggc gaattttcgc ggtggcccgc actccctgag agctcccgcc ggggcagcgg
     5701 gtcaggagtg gcatcaagga gtgactgatg tcactctcgg cgagttgatc ccatcgccgt
     5761 ggaggggacg atgaccaccg agagccagcg cagccgcacc gacgtcgaca aggccctgct
     5821 cgggcactcg acctatcgca gcctcgggga gcgacggctc tccccggcag aggagcggtt
     5881 cgagaagggc cgccgcacgg tcgggctgtt cctcgcgccg atcgtgatga tcgcgttcct
     5941 ggcgctgccg ttgaatctgc agccccagca acaggtgttg gccgggatcc tgctcggcgt
     6001 gatcgtgctg tgggtgagcg aggcggtgcc gatcccgatc gcgggcctga tcggcgtatc
     6061 gctgatcgtg atcctgcagg tggccccggc cgacgacgtg ctggcgaact tcgggtcgag
     6121 caccgtcttc acgttcatcg gggcgttcat cctcgcccag gcgatgctca agcacggggt
     6181 cgcgcgccgc ttcgccttcc ggatactggc gctgccgcgg gtcgggcggt cgagcgtcgg
     6241 ggtgatcgtc gcgttcggtg gggtcacgtg cctcctctct gcgttcgtgt cgaacaccgc
     6301 cacggtggcg atgctcctac ctacggcgct gggcatcctc agcgtgatcg cgaagctgat
     6361 gcaggaccgc gggctcgtgg ccgaggactt cgacccgctg cggctgcgtg tcggcgccgc
     6421 actgatgctg atgctggcct acggggcgag tgtgggcggc ccgctgacgc cggtcggcag
     6481 cccacccaac ctgatcggcc ggggactgat cgaggaggcc accggggagc ggatcagctt
     6541 cgcgcagtgg gtggcgctgg cggttccgat ctgtctcctg atgttcgtgg cactggccgt
     6601 cgtgctgctg ctgctcaaca agccggagat ccgccgcatc gacggcgtcg ccgagtacgt
     6661 ggtcgagcaa cgcgcccagc tgggaccgct gtcgcgtgct gagaagaaca cgttgatcgc
     6721 gttcggcgtc accgtgacgc tgtggatcct gcccgggatc gtcgcgctcg tggcgggcaa
     6781 cgactcggcc gcctacgacg cggtgagcgc acggctcgac gagggcatcg tcgcggtgtt
     6841 cggggcgtcg cctgctctac ctggcccgga cgctgggcgg ggccgtgtcg acagggctcg
     6901 ggctctcgaa cgtcatcacc atcacgatct tcgcggtgct gctggcgatc gtcgtctcgg
     6961 agaccaccag caacaccgca tcggcggccg tcgtggtacc catcgtcatc ccgatcgcgg
     7021 tggccgcggg ggtcaacccc ttcgtgcccg cgctggccgc cacgttcgcg gcgtcgttcg
     7081 ggttcatgct gccggtgtcg acgccgcaga acgcgatcgt ctacgggtcc ggcgtcgtgc
     7141 cgatcaccac gatgatccgg tccggcgcgg ctttcgacct catcggagcg atactcatca
     7201 tcgtgttcct tcctctgatg gtcgccgtcc tcgggctcgg cgtcgcatga ccccgcagac
     7261 gatcgccgtg atcccgggcg acggcatcgg atccgaggtg gtcgatgccg cccggacggt
     7321 gctggacgcc gtgtgcgccc ggcacgacat cgagctccac tacaccgagt tcgactggtc
     7381 ctgtcgccgc ttcgaacgcg agggcgcgat gatgccgccc gacgggatcg acacgctccg
     7441 cgggttcgac gcgatcctgc tcggcgcggt ggggtggccg ggcgtgcccg accacgtgtc
     7501 gctgtgggga ctgctgatcc cgatccgccg cgcgttccgg cagtacgtca acctccgccc
     7561 gatccgggtg ttcgacggcg tgcagagccc gctgcgcggc gctgaggacg tcgacttcgt
     7621 cgtcgtccgc gagaacgtcg agggtgagta cagcgaggtc ggcggccggg ccaaccgggg
     7681 gttcccggac gagatggccg tccaggagtc gatcttcacc cgggtcgggg tgagccggat
     7741 tgccgacttc gcgttcgaac tggcgcagac gaggcgcggg tacctcacgt cggcgacgaa
     7801 gtcgaacggg atcgtgcaca cactcccgtt ctgggacgag gtcgtggccg agcgggcagc
     7861 ccggtacccg gatgtgcg
//
LOCUS       DASRSC010000131         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1354990,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000131 DASRSC010000000
VERSION     DASRSC010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354990"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1664)
                     /gene="ctaD"
                     /locus_tag="VFE39_06245"
     CDS             complement(<1..1664)
                     /gene="ctaD"
                     /locus_tag="VFE39_06245"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HET6258234.1"
                     /translation="MTAVAPKPITARPYPARRSAKGSMLVKMLHTTDPKDIAILYLVT
                     SFGFFLAGGAMALLMRAELAAPGQQFLSNEQYNQLFTMHGTIMLLLYATPILFGFANY
                     IVPLQIGAPDVAFPRLNAFSYWLFLFGGLTVLSGFLTPGGAADFGWFAYTPLSDAIHS
                     PGAGADLWIVGLIMSGLGTILGGVNFITTIVCMRAPGMTMFRMPIFTWNILVTAILIL
                     IAFPVLTAALFGLLADRHFGAHVFDPANGGVILWQHLFWFFGHPEVYIVALPFFGIVS
                     EVFPVFARKPLFGYKGLIYATIAIGALSVAVWAHHMYATGSVLLAFFSFTTFLIAVPT
                     GVKFVNWIGTIWKGHLTFEPPMLFALGFLVTFLFGGLTGILLASPPLDFHVSDTYFVV
                     AHFHYVLFGTIVFATYSGIYFWFPKMTGRMMDETLGKVHFWLTFIGFHLTFLVQHWLG
                     AEGMPRRYVDYLPTDGFTTLNAISSTGAFVLGASTLPFIWNVFRSYRYGRVVTVDDPW
                     GFGNSLEWATSCPPPRHNFTELPRIRSERPAFELHYPHLVERFREEA"
     gene            complement(1827..2636)
                     /locus_tag="VFE39_06250"
     CDS             complement(1827..2636)
                     /locus_tag="VFE39_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET6258235.1"
                     /translation="MGTIVSFHAHPDDESIGSAGTLARAAAAGHRVVLVFATRGELGE
                     PVAGILEPGEQLAIRRSAECYASAAAIGAKRVEFLGYTDSGMMGEPSNDAPFCFWRAD
                     VEHAARQLAVILDEEEPDIVTIYDDNGGYGHPDHIQVHRVGRRAAELSAVPVVAQGTI
                     NRDWMTRGIRGMAKAGQLPDGWEAPAMDAPTFGKPESEITHRVDAVDFVEQKKASMRA
                     HASQIGPDHFLLAMPDPLFAMGMGVEFYIVDPLPSPAATPELFEELFTPLR"
     gene            complement(2727..>3374)
                     /locus_tag="VFE39_06255"
     CDS             complement(2727..>3374)
                     /locus_tag="VFE39_06255"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HET6258236.1"
                     /translation="RARGTRRLGPAKGLDGDLDLDRTLDGWAPGPSRRPEVDDIVTRT
                     WTAHRRAVCLVVDISGSMRGLAVALAAVAAAGVVVAAAEKLEPSVLAFGAGVRVLQAQ
                     GVRRPPEELLSDLVALRGHGLTDLAAGLREASRQLAGAVADERVVVLLSDCLHTAGGD
                     PAAALAGIDRLHVLVPLGGEDAEGAASALAARAGGRAETVRRLAEVGSALTRLLT"
BASE COUNT          582 a         1135 c         1201 g          456 t
ORIGIN      
        1 gcctcctcgc ggaagcgctc gaccaggtgc gggtagtgca gctcgaacgc cggacgctcc
       61 gagcggatcc ggggcagctc cgtgaagttg tgccgcggcg gcgggcagct cgtggcccac
      121 tccagcgagt tgccgaagcc ccacgggtcg tcgacggtga ccacgcggcc gtagcggtag
      181 ctgcggaaca cgttccagat gaacggcagc gtcgacgcgc cgagcacgaa agccccagtc
      241 gaggagatcg cgttcagcgt cgtgaacccg tcggtgggca ggtagtcgac gtagcggcgc
      301 ggcatgccct cggcgcccag ccagtgctgc accaggaacg tcaggtggaa gccgatgaac
      361 gtgagccaga agtgcacctt gcccagcgtc tcgtccatca tccggccggt catcttcggg
      421 aaccagaagt agatgccgga gtaggtggcg aacacgatcg tcccgaacag cacgtagtgg
      481 aagtgcgcca ccacgaagta ggtgtccgag acgtggaagt ccagcggcgg cgaggccagc
      541 aggatgccgg tgagcccgcc gaacaggaag gtgaccagga agcccagggc gaacagcatg
      601 ggcggctcga aggtcaggtg gcccttccag atcgtgccga tccagttgac gaacttgacg
      661 cccgtgggca ccgcgatcag gaacgtggtg aaggagaaga aggccagcag caccgagccg
      721 gtggcgtaca tgtggtgggc ccacacggcg acggacagcg cgccgatcgc gatcgtggcg
      781 tagatcaggc ccttgtagcc gaacagcggc ttgcgggcga acaccgggaa gacctcggag
      841 acgatgccga agaacggcag cgcgacgatg tagacctcgg ggtggccgaa gaaccagaac
      901 aggtgctgcc acaggatcac cccgccgttg gcggggtcga acacgtgggc accgaagtgc
      961 cggtcggcca gcagcccgaa gagcgcggcg gtgagcacgg ggaacgcgat gaggatgagg
     1021 atcgcggtca cgaggatgtt ccacgtgaag atcggcatcc ggaacatcgt catgcccggg
     1081 gcacgcatgc agacgatcgt ggtgatgaag ttgacgccac cgaggatcgt gccaagaccc
     1141 gacatgatca ggcccacgat ccagaggtcg gcacctgcgc ccggcgagtg gatcgcgtcg
     1201 gacagcgggg tgtaggcgaa ccagccgaag tcggccgccc cgcccggtgt caggaagccg
     1261 gacagcacgg tcagcccgcc gaacaggaac aaccagtacg agaaggcgtt gagccgcggg
     1321 aacgcgacgt cgggtgcacc gatctgcagc ggcacgatgt agttcgcgaa gccgaacagg
     1381 atcggcgtcg cgtacagcag cagcatgatc gtgccgtgca tcgtgaacag ctggttgtac
     1441 tgctcgttgg acaggaactg ctgccccggc gccgccagct cggcgcgcat cagcaacgcc
     1501 atggctccac cggccaggaa gaacccgaac gacgtcacca gatagaggat ggcgatgtcc
     1561 ttgggatccg tggtgtgcag cattttcacc agcatggatc ccttggccga cctgcgcgcc
     1621 gggtacgggc gcgcggtgat cggcttgggg gcgacggctg tcactgagcc tcctgggact
     1681 gcctggccga ctgcatttgc cttgccgaac tgtggggcaa ctgatcctcc gagccccgcg
     1741 ggggatggta gctctacccg tcgtcgaagg cgcgccccgg gcccggtccc ccgacgtgac
     1801 gcacctcgcg tggagagccg tgggcactac cgcagggggg tgaacagctc ctcgaagagc
     1861 tccggcgtcg ccgccgggct cggcagggga tcgacgatgt agaactcgac acccataccc
     1921 atcgcgaaca gcgggtcggg catcgcgagc aggaagtggt cgggcccgat ctggctcgcg
     1981 tgggcacgca tcgacgcctt cttctgctcc acgaagtcga cggcgtccac gcggtgggtg
     2041 atctcggact cgggcttgcc gaacgtcggc gcgtccatgg ccggcgcctc ccagccgtcg
     2101 ggcagttgcc cggccttggc catcccgcgg atgccgcggg tcatccagtc gcggttgatg
     2161 gtgccctgcg ccaccaccgg cactgccgac agctcggcgg cgcggcgccc cacgcggtgc
     2221 acctggatgt ggtcgggatg cccatagccg ccgttgtcgt cgtagatcgt gacgatgtcg
     2281 ggctcctcct cgtcgaggat caccgcgagc tgccgggccg cgtgttcgac gtcggcacgc
     2341 cagaagcaga acggggcgtc gttgctgggc tcgcccatca tccccgagtc ggtgtagccg
     2401 aggaactcga cgcgcttggc cccgatcgct gccgcggacg cgtagcactc cgcggatcgg
     2461 cggatggcca gctgctcgcc gggctccagg atgccggcca cgggttcacc cagctcaccc
     2521 cgcgtggcga agacgagtac gacccggtga cctgcggccg cggcgcgcgc gagcgtgccc
     2581 gccgaaccga tcgactcgtc gtcgggatgt gcgtggaatg agacgatggt gcccacgtag
     2641 gcgagcctat ccggcgtcca gaacgagatc gctgcgagat cctgctctcg gccctcctga
     2701 gggcacatgg ccggatctcg cgcggatcac gtgagtagtc gagtcagggc cgagcccacc
     2761 tccgcgagcc ggcgaacggt ctccgcccga ccaccggcgc gcgctgccag cgcggacgcc
     2821 gcgccctccg cgtcctcccc gcccagcggc acgagcacgt gcagccggtc gatgccggcc
     2881 agtgccgccg ccggatcgcc gcccgcggtg tgcaggcagt ccgacagcag cacgacgacc
     2941 cgctcgtcgg cgactgcccc ggcgagctgg cgtgacgcct cccgtagtcc cgccgccagg
     3001 tcggtgaggc cgtggccgcg cagggcgacg aggtcggaca acagctcctc gggcggtcgg
     3061 cggacaccct gcgcctgcag cacgcgcact ccggcaccga aggcgagcac cgacggctcc
     3121 agcttctcgg ctgccgcgac cacgaccccg gccgccgcga cggcggccag cgcgacggcg
     3181 aggccccgca tcgaaccgga gatgtcgacg acgagacaga cggcgcggcg gtgcgccgtc
     3241 caggtgcggg tgacgatgtc gtccacctcc ggccgccggg acgggcctgg cgcccacccg
     3301 tcgagcgtgc ggtcgagatc taggtcgccg tccagcccct tcgccggtcc cagcctccgc
     3361 gtgccgcggg cgcg
//
LOCUS       DASRSC010000132         8998 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3646334,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000132 DASRSC010000000
VERSION     DASRSC010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8998
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3646334"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..417
                     /gene="clpS"
                     /locus_tag="VFE39_06260"
     CDS             121..417
                     /gene="clpS"
                     /locus_tag="VFE39_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HET6258237.1"
                     /translation="MAAPLPATTRQVDPDLAEDSVVDTPWQAIVWNDPVNLMSYVTYV
                     LQKLFGYPEPKATALMLDVHHKGKAAVSAGDKDKIEADVAKLHAAGLWATMQRA"
     gene            414..965
                     /locus_tag="VFE39_06265"
     CDS             414..965
                     /locus_tag="VFE39_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 domain-containing protein"
                     /protein_id="HET6258238.1"
                     /translation="MNGWKRAGRGGRTRLVASLEAQEAAVLRGLVGEVRQILAGRTDD
                     NPADELAILTGMRTGPSTRPTDRVLARLLPDFTTEDADLAAGLRSLHEPELIEAKDSA
                     AAVVLETLPESGGRVELTPDQADAWLAALNDVRLALGTALDVSEDMPEELPPDDPRAT
                     HIGVYHWLTFVQDALVQTRMQVR"
     gene            1026..1505
                     /locus_tag="VFE39_06270"
     CDS             1026..1505
                     /locus_tag="VFE39_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HET6258239.1"
                     /translation="MLLIRADLVEEIVAHARRDHPDEACGVIAGPEGSDRPERFIPML
                     NAARSPTFYEFDSGDLLRLYRDMDDRNEVPVVIYHSHTATEAHPSRTDISLASEPEAH
                     YVLVSTRDQAESNHTGHDLRSFRIVDGVVTEEQVEVVETYMFGHTGADDVPDRDASV"
     gene            1553..1825
                     /locus_tag="VFE39_06275"
     CDS             1553..1825
                     /locus_tag="VFE39_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6258240.1"
                     /translation="MAVTVSIPTILRTHTGGEKSVEAKGSTVGEVIDDLDSRHSGIAG
                     RLVNNGSLHRFVNIYVDDEDIRFAGGLNAPVGENSNVTILPAVAGG"
     gene            1857..2807
                     /locus_tag="VFE39_06280"
     CDS             1857..2807
                     /locus_tag="VFE39_06280"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase"
                     /protein_id="HET6258241.1"
                     /translation="MARYESLLAAVGDTPLVGLPNLSPTPDVRLWAKLEDRNPTGSIK
                     DRPALAMIEKAESDGLLTPGCTVLEPTSGNTGISLAMACKLKGYTLICVMPENTSIER
                     RQLLGMYGAQIISSPAAGGSNQAVAMAKQLAAEHPDWVMLYQYGNPANADAHYRTTGP
                     EILRDLPSITHFVAGLGTTGTLVGTGRYLREHKPDVQIVAAEPRYGELVYGLRNLDEG
                     FVPELYDPEVLTSRYSVGSRDALRRTRQLVEQEGIFAGISTGGILHAALAVAEKAAGA
                     GEKADIVIVVCDAGWKYLSTGAYSGELDDAAEGIEGQLWA"
     gene            2852..3484
                     /locus_tag="VFE39_06285"
     CDS             2852..3484
                     /locus_tag="VFE39_06285"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET6258242.1"
                     /translation="MVVPASTPRRSIARVLPASPVLALFTMLVFTGVLYVSEVVDQLT
                     GLRLDADGIVPRSVDGLDGILWAPLLHAGWPHLLANTVPFLVFGFLAMAGGIRQFVIV
                     TATIWLLGGLGVWLIGPEGTSTVGASGLIFGWLVFLLARGFFARSGRQIVLAVVLFLI
                     WGGVLFGILPGAPGISWQAHLFGALAGLLAARLVVQADRRRSRPAPIAGA"
     gene            3495..4331
                     /gene="murI"
                     /locus_tag="VFE39_06290"
     CDS             3495..4331
                     /gene="murI"
                     /locus_tag="VFE39_06290"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET6258243.1"
                     /translation="MPPEIDAAIGIFDSGVGGLTVARAVIDQLPAERVVYVGDTAHSP
                     YGPLRIADVRRYALEVCDSLQERGVKALVVACNTASAACLPDIRERYSVPVVEVLRPA
                     VRRAVAATRTGRIGVIGTRATIASGAYTDSFAAARDVQVTAVACPRFVDFVERGMTSG
                     RQILGLAQSYLEPLQLARVDTVVLGCTHYPLLAGVLQIVLGPEVTLVSSADETAKDLV
                     RVLMDHDLARDPAGALHAPHEFLATGDPEPFRRLGRRFLGPEIGTVSALDTAVAAAPT
                     TG"
     gene            4382..4744
                     /locus_tag="VFE39_06295"
     CDS             4382..4744
                     /locus_tag="VFE39_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258244.1"
                     /translation="MIDALEIVVLVATGALAVFGVVLTILNRPPDRTMRLAVGAVEAL
                     LVLQAAIAAVRVFSGVTLPESTTFLIYLVVSVCVLPIATQFATAEPTRWGGTVIAVGA
                     IATLVAVVRLQGLWLAGA"
     gene            4737..5138
                     /locus_tag="VFE39_06300"
     CDS             4737..5138
                     /locus_tag="VFE39_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258245.1"
                     /translation="MPEPARATSSGPGRVLIAVYGVFALSATARALVQIASKFSEAPV
                     AYLLSALAGVVYILATIGLAGHGPAARRLAWTAVGFELAGVLVVGTLTVLDTADFPDD
                     TVWSVFGRGYGFVPLVLPLVGLWWLRHTRAR"
     gene            5147..5932
                     /locus_tag="VFE39_06305"
     CDS             5147..5932
                     /locus_tag="VFE39_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6258246.1"
                     /translation="MRLIVLGCSGSGPGPASPASGYLVEAGDARLVMDLGNGTLGALQ
                     RHLDPWELDAVVFSHLHPDHCADFSSLVVHRRYHPRPPFDPAKRRLPVYAPAEAPDRF
                     AAAYAASAAERATTDLGDVFAFLPLSDGGDTAVAGAVLRAGLVDHPCEAYALRLEHGG
                     RSLVYSGDTGPCEGLVELARGADVLLCEATWPHVTEWWAQPPPGVHLSGRQAGEHAAA
                     AGVGRLLLTHVPAWFDGKQLLAEAKEAFDGPVELVVPDAGYTI"
     gene            6025..6783
                     /gene="rph"
                     /locus_tag="VFE39_06310"
     CDS             6025..6783
                     /gene="rph"
                     /locus_tag="VFE39_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HET6258247.1"
                     /translation="MDGTRADGRSDDELRPVTITRGFQKHPAGSVLVEFGDTKVLCAA
                     SVSEGVPRWRKGSGLGWLTAEYAMLPSATNTRNDRESVKGRIGGRTHEISRLIGRSLR
                     ACIDLAALGENTIALDCDVLQADGGTRTAAITGAYVALADAVTWLGAHGSLADPKPLS
                     CRVAAVSVGVVDGRVRLDLPYAEDSRAEVDTNVVATDTGTLIEVQGTGEGATFTRRTL
                     DAMLDVALAGIRQLSDIQAAALAEPYPGVLPEHA"
     gene            6810..7406
                     /gene="rdgB"
                     /locus_tag="VFE39_06315"
     CDS             6810..7406
                     /gene="rdgB"
                     /locus_tag="VFE39_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005440422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HET6258248.1"
                     /translation="MANTVLVATRNAGKLAELRRMLAGGPVEIVGLADVPAFPDAPET
                     GATFAENALAKARDAAAATGLPAVADDSGLTVEALNGMPGVLSARWSGRHGDDRANLE
                     LLLAQLADVPDERRGAAFVCAAALAVPGGLETVVHGEWPGRIVRAPRGSGGFGYDPIF
                     VPDGDERTSAELSPEEKDAASHRGRAFRALLPHLRDLA"
     gene            complement(7424..7831)
                     /locus_tag="VFE39_06320"
     CDS             complement(7424..7831)
                     /locus_tag="VFE39_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET6258249.1"
                     /translation="MTTEQPQPPVLDLDPDGLAVRREVLGPDHVDAALARADATTAEF
                     QEMITRYAWGHIWTRPGLDRRMRSAITITALVAHGHYAELELHLRAALRNGLTREEIV
                     EVLLQSAIYCGVPAANSAFGVARRVLDEEPTTS"
     gene            7959..8651
                     /locus_tag="VFE39_06325"
     CDS             7959..8651
                     /locus_tag="VFE39_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET6258250.1"
                     /translation="MKWGRIAAGVAALAVVTTAAVLLRDGIPDVRQTVQSAGLWAPVL
                     FVLLQGMVTVTPVPRTVFTVAAGVLFGSVVGLLLTVAGTALAAVVAFWLVRLVGGRLA
                     ERHAQHRGVAWVRARLDHSGLLAVLSLRLIPAVPFSAMNYAAGLSGVRFAPYLLGTVL
                     GVLPGTIGIVVLGDAVVGGSPHPAMFAVSVASGLLGLAGALVVARRQPAVASATPTST
                     AVQQEPGTDQPG"
     gene            complement(8665..8737)
                     /locus_tag="VFE39_06330"
     tRNA            complement(8665..8737)
                     /locus_tag="VFE39_06330"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8702..8704),aa:Leu,seq:tag)
BASE COUNT         1177 a         3214 c         3239 g         1368 t
ORIGIN      
        1 ccatcgcatg acgtcactgc gcggaggcgc tgtgtcggtg attcgcttgc gcgctcgctc
       61 atggacctgg accctatgtt gccgtgcgcc ttcgatggcc ggggtgtcac catggaccgc
      121 atggccgcgc cgctacccgc aaccacgcgc caggtggatc cggacctcgc agaggactcg
      181 gtggtcgaca cgccctggca ggcgattgtg tggaacgacc ccgtgaacct catgtcctac
      241 gtcacctacg tgctgcagaa gctcttcggc tacccggagc cgaaggccac cgcgctgatg
      301 ctcgacgtgc accacaaggg caaggcggcg gtgtcggcag gcgacaagga caagatcgag
      361 gccgacgtcg cgaagctgca cgccgcgggc ctgtgggcaa ccatgcagcg cgcatgaacg
      421 ggtggaagcg tgccggccgg ggtggtagga cccggctcgt cgcgtcgctg gaggcgcagg
      481 aggccgccgt gctgcgcggc ctggtcgggg aggtccggca gatcctcgcc gggcgcaccg
      541 acgacaaccc cgccgacgag ctcgcgatcc tcaccggcat gcgcacgggg ccgtccaccc
      601 gccccaccga ccgggtcctc gcccggctgc tccccgactt caccaccgag gacgccgacc
      661 tcgccgccgg tctgcggtcc ctgcacgagc cggagctgat cgaggccaag gactcggccg
      721 cggccgtggt gctggagacg ttgccggagt ccggcggtcg ggtggagctc acgcccgacc
      781 aggccgacgc ctggctggcc gcgctcaacg acgtgcggct cgccctcggg accgccctcg
      841 acgtcagcga ggacatgccg gaggaactcc cacccgacga tccgcgcgcc acacacatcg
      901 gcgtctacca ctggctcacc ttcgtgcagg acgcgctggt gcagacccgg atgcaggtgc
      961 gctagcaatg tgagtccgcc cgccgcggtg tgcggtacgg gccgatgggg tcgtacgctg
     1021 gttgagtgct gttgatccga gccgatctcg tcgaggagat cgtcgcccac gcccgccggg
     1081 accatcccga cgaggcgtgc ggcgtcatcg ccggacccga gggctcggac cggccggagc
     1141 ggttcatccc catgctgaac gcggctcgct cgccgacgtt ctacgagttc gactcggggg
     1201 acctgcttcg gctgtaccgc gacatggacg accgtaacga ggtgcccgtg gtgatctacc
     1261 actcacacac ggccacggag gcccatccgt cgcgcaccga catctccctc gcgtccgagc
     1321 ccgaggcgca ctacgtactc gtgtccacgc gggatcaggc cgagtcgaac cacaccgggc
     1381 acgacctccg gtccttccgg atcgtcgacg gtgtggtcac cgaggaacag gtcgaggtcg
     1441 tggagactta catgttcggg cacaccggcg cggacgacgt ccccgacagg gacgcgtcgg
     1501 tctgacgcca cctgccacga cccgtcccga acgaccgacc tggagcatca ccatggccgt
     1561 caccgtgtcc atccccacca tcctccgcac tcacaccggc ggcgagaagt ccgtcgaggc
     1621 gaagggcagc accgtcggcg aggtgatcga cgacctggat tcgcggcaca gcggcatcgc
     1681 cgggcgcctg gtcaacaacg gctcgctgca ccggttcgtc aacatctacg tcgacgacga
     1741 ggacatccgc ttcgcgggcg gcctcaacgc gccggtgggg gagaactcca acgtcacgat
     1801 cctgcctgcc gtcgcaggcg gctgaggtac ggaccgaggc acgggaaagg ccggacatgg
     1861 cccgctacga gtcgctgctg gccgctgtcg gggacacccc gctcgtcggc ctgccgaacc
     1921 tctcgccgac gccggacgtg cggctgtggg cgaagctcga ggaccgcaac cctaccggct
     1981 cgatcaagga ccgccccgcg ctggccatga tcgagaaggc ggagtcggac ggcctgctga
     2041 cgcccggctg cacggtgctc gagcccacgt cgggcaacac cggcatctcg ctcgcgatgg
     2101 cctgcaagct caagggctac acgctgatct gcgtgatgcc ggagaacacc tcgatcgagc
     2161 gcaggcagct gctcgggatg tacggcgcgc agatcatctc ctcgcccgct gctggcggct
     2221 ccaaccaggc cgtggccatg gccaagcagc tcgcagcgga gcacccggac tgggtgatgc
     2281 tctaccagta cggcaacccg gccaacgccg acgcgcacta ccgcaccacg ggcccggaga
     2341 tcctgcgcga cctgccctcg atcacgcact tcgtcgcggg gctcggcacc acgggcactc
     2401 tcgtgggcac cgggcgctac ctgcgggagc acaagccgga cgtgcagatc gtggcggccg
     2461 agccgcgcta cggcgagctg gtctacgggc tgcgcaacct cgacgagggc ttcgtccccg
     2521 agctctacga ccccgaggtg ctcacctcgc gctactccgt gggctcgcgc gacgctttgc
     2581 ggcgcacccg tcagctcgtc gagcaggagg gcatcttcgc gggcatctcc accgggggca
     2641 tcctgcatgc cgcgctggcc gtcgcagaga aggccgcggg tgcgggcgag aaggccgaca
     2701 tcgtcatcgt cgtgtgcgac gccggctgga agtacctgtc cacgggcgcc tactccggcg
     2761 agctggacga cgctgcggag ggcatcgagg gccagctctg ggcctgagaa cgtcctccgg
     2821 gacgcaccga ggtgcgggta gcctcggtcg catggtcgtc cctgcgtcca cgccccggcg
     2881 gtcgatcgcc cgggtgctcc cggcctcgcc ggtactcgca ctgttcacca tgctcgtgtt
     2941 caccggggtg ctctacgtgt ccgaggtggt cgaccagctc accgggctgc gtctcgacgc
     3001 cgacggcatc gtgccgcgct cggtcgacgg gctcgacggg atcctctggg ccccgctgct
     3061 gcacgccggg tggccacacc tgctggccaa caccgtgccg ttcctcgtct tcggcttcct
     3121 cgccatggcg ggggggatcc ggcagttcgt catcgtcacc gccacgatct ggctgctggg
     3181 cgggctcggc gtgtggctga tcggcccgga gggcacctcc acggtcggcg cgtccgggct
     3241 gatcttcggg tggctggtgt tcctgctcgc ccgcgggttc ttcgcccgca gcggccggca
     3301 gatcgtgctc gcggtggtgc tgttcctgat ctggggcggc gtcctgttcg ggatcctgcc
     3361 gggcgcgccg ggcatctcct ggcaggcgca cctgttcggg gcgctcgccg gcctactcgc
     3421 cgcccggctc gtcgtgcagg ccgaccgacg ccggtcgcgg ccggccccga tcgcgggcgc
     3481 ctaagctgct caccgtgccc ccagagatcg acgcggcgat cggcatcttc gactcggggg
     3541 tcggtggcct cacggtcgcc cgagcggtga tcgaccagct ccccgccgaa cgggtcgtct
     3601 acgtcggcga caccgcgcac agcccgtacg ggccgttacg gatcgctgac gtgcgccgat
     3661 acgcgctcga ggtctgcgac tcgctgcagg agcgtggggt caaagcgctc gtcgttgcct
     3721 gcaacaccgc ctcggcggcc tgcctcccgg acatccgcga gcggtactcg gtgccggtcg
     3781 tcgaggtctt gcgccctgcc gtccgccgtg ctgtcgccgc cacccgtacc ggtcggatcg
     3841 gggtcatcgg cacccgcgcc accatcgcgt ccggggcgta caccgactcg ttcgcggccg
     3901 cccgcgacgt ccaggtcacg gccgtggcgt gcccccgctt cgtcgacttc gtcgagcgcg
     3961 gcatgaccag cggacgccag atactgggcc tcgcccagag ctacctcgag ccgctgcagc
     4021 tcgccagggt ggacacggtc gtgctcggct gcacccacta cccgctgctc gcgggcgtgc
     4081 tgcagatcgt gctcgggccc gaggtcacgc tggtgtccag cgccgacgag acggcgaagg
     4141 acctcgtgcg ggtgctcatg gaccacgacc tcgcgcggga ccccgccggg gcgctgcacg
     4201 ccccccacga gttcctcgcc accggcgacc cggagccgtt ccgccggctc gggcgccggt
     4261 tcctcggtcc ggagatcggt acggtctcgg cgctcgacac cgccgtggcg gccgccccga
     4321 ccaccggctg acccgggcct ggcccggatc cgcggctgcg ctgcgggtag cgtcgtgaat
     4381 cgtgatcgac gcgctggaga tcgtggtgct cgtcgcgacc ggagccctgg ctgtgttcgg
     4441 ggtggtgctc acgatcctga accgcccccc ggaccgaacc atgcggctcg cggtcggtgc
     4501 cgtcgaggca ctgctcgtcc tgcaggcggc catcgcggcc gtccgggtgt tctcgggcgt
     4561 cacgctgccc gagagcacga cgttcctgat ctatctggtc gtgtcggtgt gcgtgctgcc
     4621 gatcgccacg cagttcgcca cggccgagcc cacgcgctgg ggcggcaccg tcatcgccgt
     4681 cggcgcgatc gccaccctcg tcgccgtggt gcggctgcag gggctgtggt tggccggtgc
     4741 ctgagcccgc tcgcgccacg tcgtcgggcc ccggccgggt gctgatcgcc gtctacggcg
     4801 tgttcgccct ctccgccacc gcccgtgccc tcgtgcagat cgcctcgaag ttcagcgagg
     4861 ccccggtggc atacctgctg tccgcgctgg cgggcgtcgt ctacatcctg gcgacgatcg
     4921 ggctggccgg tcacgggccc gccgcccgca gactggcgtg gaccgccgtc gggttcgagc
     4981 tggcgggcgt actggtcgtc gggacgctca ccgtgctcga caccgctgac ttccctgacg
     5041 acaccgtgtg gtcggtgttc gggcggggct acggcttcgt gccgctggtt ctgccgctcg
     5101 tcggcctctg gtggctgcgc catacccgcg cgcgttaacg tgcggcatga ggctgatcgt
     5161 gctcggctgc tctggcagcg gccccggtcc ggcctcgccg gcgtcgggct acctcgtcga
     5221 ggcgggtgac gcgcgcctgg tgatggatct gggcaacgga acgctgggcg cgctgcaacg
     5281 ccacctcgac ccgtgggagc tcgacgccgt ggtgttcagc cacctccacc ccgaccactg
     5341 cgccgacttc tcgtccctgg tcgtccaccg ccgctaccac ccgcgcccgc cgttcgaccc
     5401 cgccaagcgc cgcctgcccg tgtacgcccc agccgaggca cccgaccgtt tcgcagccgc
     5461 ttatgcggcg tcggcggccg agcgggccac gaccgacctc ggcgacgtct tcgcgttcct
     5521 ccccttgtcc gacggtgggg acaccgccgt ggccggagcc gtgctgcggg ccggactggt
     5581 ggaccacccg tgcgaggcgt atgccctgcg tctcgagcac ggcggtcgca gcctcgtcta
     5641 cagcggcgac accggtccct gcgaggggct cgtcgagctt gcgcgcggcg ccgacgtgct
     5701 gctctgcgag gccacgtggc cgcacgtcac cgagtggtgg gcgcagccgc ctcccggcgt
     5761 gcacctgtcg ggccggcagg cgggcgagca cgcggcggct gccggtgtcg ggagactgct
     5821 gctgacccac gtcccggcct ggttcgacgg gaagcagttg ctcgccgagg cgaaggaggc
     5881 gttcgacggg cccgtcgagc tggtcgtccc tgatgccgga tacacgatct gattcgcgac
     5941 cactgccacg gtgagggcgc cggtccagcg acctaagctg gcgcggtgac aagggcggat
     6001 gggacgcgaa cggatggcac ttccatggac ggtacgaggg cggacggacg gtcggacgac
     6061 gagctccgcc cggtgacgat cacccgcggc ttccagaagc atccggcggg ttcggtgctc
     6121 gtcgagttcg gggacacgaa ggtgctgtgc gcggcaagcg tcagcgaggg agtgccgcgc
     6181 tggcgcaagg ggtcggggct cggctggctc accgcggagt acgcgatgct gccctcggcc
     6241 accaacacgc gcaacgaccg cgagtcggtg aagggccgca tcggcggccg cacccacgag
     6301 atcagcaggc tgatcggccg ctcgctgcgg gcatgcatcg acctggcggc gctcggcgag
     6361 aacaccatcg cgctggactg cgacgtcctg caggccgacg gcggcacccg cacagcggcg
     6421 atcacgggtg cctacgtcgc gctggccgac gctgtcacct ggctcggcgc gcacggcagc
     6481 ctcgccgacc ccaagccgct gtcgtgccgg gtcgcggccg tgagcgtcgg ggtcgtcgac
     6541 ggccgggtgc gcctcgatct gccctatgcg gaggactccc gcgccgaggt cgacacgaac
     6601 gtcgtcgcca cagacaccgg caccctcatc gaggtgcagg gcaccgggga gggcgccacg
     6661 ttcacccgcc gcaccctcga cgccatgctc gacgtcgcgc tcgccggcat ccggcagctt
     6721 tccgacatcc aggccgccgc actcgccgag ccctaccccg gtgtgctgcc ggagcatgcg
     6781 tgagtgccgg ggccgcggag gagggcgggg tggccaacac cgtgctggtg gccacacgca
     6841 acgccgggaa gctggcggag ctgcgtcgga tgctcgccgg cggccccgtc gagatcgtcg
     6901 gtctcgcgga cgtgccggcg ttccccgacg ccccggagac gggtgcgacg ttcgcggaga
     6961 acgcactcgc caaggccagg gacgcggcgg ccgccaccgg ccttcccgcg gtcgcggacg
     7021 actctggcct gaccgtcgag gcgttgaacg ggatgccggg cgtgctgtcg gcgcgctggt
     7081 cggggcggca cggggacgac cgcgccaacc tggagctgct gctcgctcag ctcgccgacg
     7141 tgcccgacga gcggcgtggt gcggcgttcg tctgcgcggc ggcgctcgcg gtgcccggcg
     7201 ggctggagac cgtggtgcac ggtgagtggc cgggccgcat cgtccgtgcg cccaggggat
     7261 ccggcgggtt cggttacgac ccgatcttcg tgcccgacgg ggacgagcgc acctccgccg
     7321 agctgagccc ggaggagaag gacgcggcct cgcaccgggg ccgcgcattc cgcgccctcc
     7381 tcccgcacct gcgcgatctc gcctgaccgc gccgcggttg gtctcaggag gtcgtgggct
     7441 cctcgtcgag gacacggcgg gcgacgccga acgcggagtt ggccgcgggc acgccgcagt
     7501 agatggcgct ctgcagcagc acctccacga tctcctcgcg ggtgaggccg ttgcgcaggg
     7561 cagcgcgcag gtgcagctcc aactcggcgt agtgcccgtg cgcgaccagg gccgtgatcg
     7621 tgatcgcgct gcgcatccgg cggtccagcc ccgggcgggt ccagatgtgg ccccaggcat
     7681 agcgggtgat catctcctgg aactcggccg tggtggcgtc ggcgcgggct agcgccgcgt
     7741 cgacgtggtc gggccccagc acctcgcggc gtacggcgag cccgtccggg tcgaggtcga
     7801 gaacgggcgg ttgcggttgc tcggtcgtca cgatcgttca cggtagccgc cggggtgggg
     7861 accgaccggt agcggcgtga tgaccggcag atgagcggcc gatggcgcgc tcaccccatc
     7921 gtcacgcggc tggtgcgggc gtcggtaccg tcggcgacgt gaagtgggga cggatcgcgg
     7981 caggcgtcgc cgcactggcg gtcgtgacga ccgccgccgt cctcctgcgc gacgggatcc
     8041 ccgacgtgcg gcagacggtc cagagtgccg gcctctgggc gccggtgctg ttcgtgctgc
     8101 tccagggcat ggtcaccgtg acgcccgtgc cgcgcacggt gttcaccgtg gccgcaggag
     8161 tgctgttcgg ctcggtcgtg gggctcctgc tcaccgtcgc cggcacggcg ctggctgcag
     8221 tggtggcctt ctggctggtt cggctggtcg gcggcagact ggcggagcgg cacgcgcagc
     8281 accgtggagt ggcctgggtg cgcgcccggc tggaccacag cgggctcctc gccgtgctgt
     8341 cgctgcgcct gatcccggcc gtgccgttct cggcgatgaa ctatgccgcc gggctgtcgg
     8401 gagtgcgctt cgcgccgtac ctgctgggca cggtgctcgg cgtactcccc ggcacgatcg
     8461 ggatcgtggt gctgggcgat gcggtggtcg gcggcagccc gcatcccgcg atgttcgccg
     8521 tgtcggtggc gtccggcctg ctggggctgg ccggggcgct ggtggtggcg cgccggcagc
     8581 cggcggtcgc gtccgccact ccgacgtcca cggccgtgca gcaggaaccc gggacggacc
     8641 agcccggata gctggaaaaa cctgtgccgg cggtgggatt cgaacccaca ctgacggact
     8701 cctagggccc gtgcctctgc cgttgggcta cgccggctcg actgcgccca caccaggtga
     8761 cggtcagggc gtgacagttg ggacacagga tacggaggtt ctcgagccgg ttgtcagtgt
     8821 ggtcaccatt gatgtggtcg agcgcgaggg gaagggggct gcctcgccat tcctggaggc
     8881 cacactcctg gcagtggtcg ggtagcagtc cggcagtttg gagccggact cgcaaggagc
     8941 tcgagcctat ggtggagccg cgcaccagca gctcatcgag gggcgtgagg cgtttcga
//
LOCUS       DASRSC010000133         9754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_373636,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000133 DASRSC010000000
VERSION     DASRSC010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9754
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373636"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /gene="carB"
                     /locus_tag="VFE39_06335"
     CDS             complement(<1..527)
                     /gene="carB"
                     /locus_tag="VFE39_06335"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675616.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HET6258251.1"
                     /translation="MPKRDDIRHVMVIGSGPIVIGQACEFDYSGTQACRVLQAEGIRV
                     SLVNSNPATIMTDPEFADATYIEPITPEFVEMVIAKERPDAILATLGGQTALNTAIAL
                     HENGVLERYGVELIGADVEAIQRGEDRLKFKEIVQSIGGDVPRSAVCHTMAEVRAAVV
                     ELGLPVVIRPSFTMGG"
     gene            complement(527..1669)
                     /gene="carA"
                     /locus_tag="VFE39_06340"
     CDS             complement(527..1669)
                     /gene="carA"
                     /locus_tag="VFE39_06340"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET6258252.1"
                     /translation="MSDEALLVLEDGRIFRGAAYGARGEGLGEAVFTTGMTGYQETLT
                     DPSYHRQIVVQTAPQIGNTGWNDEDDESVRIQVAGYAVRDPARSPSSWRSRRSLGDAL
                     AEQGIVGVAGIDTRALVRHLRERGAMRAGVFSGGALAAADDLVRRVLDSPPMEGADLY
                     GAVTTREPYVVPAAGTPRFRVAALDVGIKSNTPRMLAARGVEVHVLPADTTIEQLEAL
                     APDGFFLSNGPGDPATAEGPVALTQQVLERRIPLFGICFGNQILGRALGRGTYKMRYG
                     HRGINIPVVEHATGRVAITSQNHGFAVEGEAGELFDTPFGRAQITHTCPNDGTVEGLA
                     ALEVPAFSVQYHPEAAAGPHDAADLFDRFVELMGAHRGPATHEEGA"
     gene            complement(1666..3153)
                     /locus_tag="VFE39_06345"
     CDS             complement(1666..3153)
                     /locus_tag="VFE39_06345"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET6258253.1"
                     /translation="MRAPHRTAATTSADLLITNARPYGEDAVDLLVRDGIVTEIGRGL
                     AGPDGIETLDAGGHVLLPGFVDLHTHLREPGGEESEDVATGSAAAALGGYTAVFAMPN
                     TDPVADTAVVVEHVRRRGEEVGLVDVHPVGAVTVGLGGLQMAELGTMARSRAQVRMFS
                     DDGRCVHDPLIMRRALEYASALDVVIAQHAEDHRLTAGAQAHEGVVASRLGLAGWPAT
                     AEETIVARDCALAREAGGALHVCHVSSARTIDVLRAAKAAGVRVSAEVTPHHLLLTDA
                     ALTSYDPVHKVNPPLRTGADTRAMREALAEGVIDVVATDHAPHATQYKDTEWAAARPG
                     MLGLQTALSVVVLAVVEPGLLDWRGVARVLSERPAEIAGLPDHGRPIAAGEPANFALV
                     DPDGVWTVRGATLASRASNTPYEGMRLPATVVATVLRGRLTSRSGRVAGIDAERTERV
                     GGIDAERSGRVTDIDAAHSAREANIGAARSGREAPVGAPRNGEHQ"
     gene            complement(3150..4094)
                     /locus_tag="VFE39_06350"
     CDS             complement(3150..4094)
                     /locus_tag="VFE39_06350"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET6258254.1"
                     /translation="MKHLLSVTDLDAATATGLLDTADRLKQALLGREVRKLPTLRGRT
                     MITMFYEDSTRTRVSFEIAGKWMSADTVNVSAKGSSVSKGESLRDTSLTLSAMGADCV
                     IVRHPASGAAHRLAGWIDRDQATTGTYTSIVNAGDGTHEHPTQALLDAATLRDRLGGI
                     AGRRIGIVGDVLHSRVARSNVFLLATLGAEVVLVAPPTLLPVGVDEWPCRVGTELDAE
                     LPALDAVMMLRVQAERMHGSFFPSAREYAIGYGLSTERAALLPEHAVVLHPGPMVRGM
                     EIAPSVADSASAAILAQVSNGVHVRMAVLYHLLAGAPE"
     gene            complement(4091..4675)
                     /gene="pyrR"
                     /locus_tag="VFE39_06355"
     CDS             complement(4091..4675)
                     /gene="pyrR"
                     /locus_tag="VFE39_06355"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET6258255.1"
                     /translation="MADDRRGDPAGATSPARELLSAADVTRTIARIAHQIIEKTAGAS
                     GVVLIGIPTRGTVLARRLADAIAEFEGSGPPVGSVDPTLYRDDLRRRPARALEDTAVP
                     DGGLDDALVVLVDDVLMSGRTVRAALDALSDHGRPRAVQLAVLVDRGHRELPIRADYV
                     GKNVPTARDEQILVLLSEIDGSDGVRLRRAGEDA"
     gene            5160..5642
                     /locus_tag="VFE39_06360"
     CDS             5160..5642
                     /locus_tag="VFE39_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET6258256.1"
                     /translation="MGDYAKALGGKLRAIRQQQGLSLHGVEQKSGGRWKAVVVGSYER
                     GDRAVTVQKLAELADFYGVPVAELLPEGRVPSGSEPATKIVINLERLQQLPADKVGPL
                     ARYAAAIQSQRGDYNGKVLSIRAEDLRSLSIIYDMTPGELTDQLIEWGVLPPEARPSA
                     "
     gene            complement(5729..6148)
                     /gene="nusB"
                     /locus_tag="VFE39_06365"
     CDS             complement(5729..6148)
                     /gene="nusB"
                     /locus_tag="VFE39_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET6258257.1"
                     /translation="MRARTKARKRALDILFESEARGDDPLTVLTQRRETDDAPPVSDY
                     AAMLVDGVVEHRERIDQLLAEHAEGWTVARMPAVDRTLLRIGVFELLWVDEIDDPVAI
                     TEAVELARTLSTDDSPRYLNGVLGRIADIAEHLRATR"
     gene            complement(6168..6731)
                     /gene="efp"
                     /locus_tag="VFE39_06370"
     CDS             complement(6168..6731)
                     /gene="efp"
                     /locus_tag="VFE39_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET6258258.1"
                     /translation="MATTNDLKNGLVLNLDGQLWSVTAFQHVKPGKGGAFVRTTLKNV
                     LTGKVVDKTFNAGTKVDTATVDRRDMTFLYRDGADFVFMDSDTFDQIPIPEGTVGDAA
                     NYLLENQTAMVALHDGVPLYVELPTSVELVISHTDPGLQGDRSTGGTKPATVETGAEI
                     QVPLFLTTGEKVKVDTRDGRYLGRVSG"
     gene            complement(6872..7954)
                     /locus_tag="VFE39_06375"
     CDS             complement(6872..7954)
                     /locus_tag="VFE39_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET6258259.1"
                     /translation="MFRSRRTALRGLLRAAEVDALMVTDLINIRYLTGFTGSNAALLV
                     HADGDTSTRFCTDGRYRTQAAEQVPDLQTVIERGSALALACSARSWGVRRLGFESDAV
                     TVDAHAAMAEAADGVVLHRAPGLVQQLRVVKDDVEIAAVRAACAAADAALADLLAAGG
                     LRPGRTEREVALDLENRMRRHGAAGPSFETIVAAGAHSAVPHHSPTEAELRAGDLVKM
                     DFGALVDGYHSDMTRTVVLGPAAAWQRELHALVAAAQAAGRAALAPGAEVSEVDGAAR
                     RVVDAAGRGPDFMHGLGHGVGLQIHEAPALSKTATGVLAARMVVTVEPGVYLEGRGGV
                     RIEDTLVVRDGAPDLLTCTSKELVEV"
     gene            8130..8348
                     /locus_tag="VFE39_06380"
     CDS             8130..8348
                     /locus_tag="VFE39_06380"
                     /inference="COORDINATES: protein motif:HMM:NF039788.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6458 family protein"
                     /protein_id="HET6258260.1"
                     /translation="MRIGSSIGLIAIGLILALAINVNLSGIDLTLVGWILAAVGVVGL
                     IASLALARRNRPVVVAREEYPPPQQRGY"
     gene            complement(8358..8810)
                     /gene="aroQ"
                     /locus_tag="VFE39_06385"
     CDS             complement(8358..8810)
                     /gene="aroQ"
                     /locus_tag="VFE39_06385"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HET6258261.1"
                     /translation="MSEPQPVLVLNGPNLGRLGTREPAVYGATTYADLVALCERTGRE
                     LGLAVEVRQTDHEGELLGWLHGAADASTPVVLNAAAWTHTSVAVRDACAQLTAPLVEV
                     HISNVHAREEFRHHSYISAVASGVIVGLGMGGYGLALRWIAEQARPDQ"
     gene            complement(8835..>9754)
                     /gene="aroB"
                     /locus_tag="VFE39_06390"
     CDS             complement(8835..>9754)
                     /gene="aroB"
                     /locus_tag="VFE39_06390"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET6258262.1"
                     /translation="CDLVDAGVDAHRVELPDAEDGKALTVAGFCWEVCGRIGLTRGDV
                     VISLGGGAATDLAGFVAATWMRGVRVVHVPTTLLAMVDAAVGGKTGINTAAGKNLVGA
                     FHEPSAVLVDLAVLDTLPAAELAAGSAEIVKAGFIADPVILDLFEADPAAALDPAGVV
                     LPELVRRSIQVKADVVAADLRESHLREILNYGHTLAHAIERREDYRWRHGAAVSVGLV
                     FAAELARAAGRLDEATAARHRAILQSLGLPVSYERGALPELVQTMRGDKKSRAGMLRF
                     VVLDGLAAPSRLEGPAPELLETAYAAVSQ"
BASE COUNT         1334 a         3584 c         3432 g         1404 t
ORIGIN      
        1 ccgcccatcg tgaacgacgg ccggatgacg accggcagcc ccagctcgac gaccgcggcc
       61 cgcacctcgg ccatcgtgtg gcagaccgcg gagcggggca cgtcgccgcc gatcgactgc
      121 acgatctcct tgaacttcag ccggtcctcg ccgcgctgga tggcctcgac gtcggcaccg
      181 atgagctcca cgccgtagcg ctccagcacg ccgttctcgt gcagggcgat cgcggtgttg
      241 agcgcggtct gcccgccgag cgtggccagg atggcgtccg gccgctcctt ggcgatcacc
      301 atctccacga actccggggt gatcggctcg atgtaggtgg cgtcggcgaa ctcggggtcg
      361 gtcatgatcg tcgccgggtt ggagttgacc agcgacaccc ggatgccctc ggcctgcagc
      421 acgcggcagg cctgggtgcc ggagtagtcg aactcgcacg cctggccgat gacgatcggt
      481 ccggagccga tcaccatcac gtgccggatg tcgtcgcgct tcggcatcag gcgccttcct
      541 cgtgcgtagc ggggcctcgg tgggcaccca tcagctccac gaaccggtcg aacaggtccg
      601 cggcgtcatg cgggccggcc gcggcctcgg ggtggtactg cacgctgaac gccgggacct
      661 cgagcgccgc caatccttcg accgtgccgt cgttcgggca ggtgtgggtg atctgcgccc
      721 gcccgaacgg cgtgtcgaag agctcccccg cctcgccctc caccgcgaac ccgtggttct
      781 gcgacgtgat cgcgacccgg ccggtggcgt gctcgaccac cgggatgttg atgccacggt
      841 ggccgtagcg catcttgtag gtgccgcgac cgagcgcccg gccgaggatc tggttgccga
      901 agcagatccc gaacagcggg atgcgccgct ccagcacctg ttgcgtgagc gccacgggcc
      961 cctccgcggt ggccgggtca cccgggccgt tggacaggaa gaacccgtcg ggcgcgaggg
     1021 cctcgagctg ctcgatcgtc gtgtcggccg gcagcacgtg cacctcgacg ccgcgcgcgg
     1081 ccagcatgcg cggggtgttc gacttgatcc cgacgtccag cgccgccacc cggaaccggg
     1141 gcgtcccggc ggcgggcacg acgtacggct cgcgggtggt gaccgcgccg tagaggtcgg
     1201 cgccctccat cggcggactg tcgaggacgc ggcgaaccag gtcgtcggcc gcggccagcg
     1261 caccaccgga gaacacgccg gcacgcatgg caccgcgctc gcgcaggtgg cgcaccagcg
     1321 cgcgggtgtc gatgccggca acgccgacga tcccctgctc ggcgagcgcg tcgccgaggg
     1381 agcgccggga gcgccagctc gatggcgacc gggccgggtc acgcacggcg tagcccgcca
     1441 cctggatacg cactgactcg tcgtcctcgt cgttccagcc ggtgttgccg atctgcggcg
     1501 ccgtctgcac cacgatctgg cggtggtagg acgggtcggt cagcgtctcc tggtagcccg
     1561 tcatgccggt ggtgaacacg gcctcgccga gcccctcgcc gcgcgcgccg tatgccgcgc
     1621 cgcggaagat ccggccgtcc tcgagaacga gtagggcttc gtcgctcatt ggtgctctcc
     1681 gttccgcggg gcgccgacgg gcgcttcccg cccgctccgc gccgcgccga tgttcgcctc
     1741 gcgcgcgctg tgggccgcgt cgatgtcagt tacccgtccg ctccgctcgg cgtcgatgcc
     1801 ccctacgcgt tcggttcgct ccgcgtcgat ccccgctacg cgtccgctcc gcgaagtgag
     1861 ccggccgcgc agcaccgtcg cgaccaccgt cgccggcagc cgcatgcctt cataaggcgt
     1921 gttggacgcc ctgctcgcca gcgtggcgcc gcgcaccgtc cagaccccgt cggggtcgac
     1981 gagcgcgaag ttcgccggct cgcccgcagc gatcgggcgg ccgtggtcgg gcagcccggc
     2041 gatctccgcc ggccgctccg acagcacgcg cgccacgccg cgccagtcca gcaaccccgg
     2101 ctcgaccacc gcgagcacca ccaccgagag tgccgtctgc agccccagca tgccgggccg
     2161 ggccgcggcc cactcggtgt ccttgtactg ggtcgcgtgc ggggcgtggt cggtcgcgac
     2221 gacgtcgatg acgccctcgg cgagcgcctc gcgcatcgcg cgggtgtcgg ccccggtgcg
     2281 caacggcggg ttgaccttgt gcaccgggtc gtagctggtg agcgccgcgt cggtgagcag
     2341 cagatggtgc ggggtgacct cggcgctcac ccgcacgccc gccgccttgg ccgcgcgcag
     2401 tacgtcgatc gtgcgtgccg acgagacgtg gcagacgtgc agcgcgcccc cggcctcccg
     2461 ggcgagcgcg cagtcgcggg cgacgatcgt ctcctccgcg gtggcgggcc accccgcgag
     2521 cccgagccgg gatgcgacga cgccctcgtg cgcctgcgcg ccggccgtca gccggtggtc
     2581 ctcggcgtgc tgggcgatga cgacgtcgag cgcggaggcg tactcgagcg cgcggcgcat
     2641 gatcagcggg tcgtgcacgc agcggccgtc gtcgctgaac atccgtacct gggcgcgcga
     2701 gcgcgccatc gtgccgagct cggccatctg caggccaccg agccccacgg tcaccgcgcc
     2761 gaccgggtgc acgtcgacca gcccgacctc ctcgccgcgc cggcgcacgt gctcgacgac
     2821 gacggccgtg tcggcaaccg gatcggtgtt cggcatcgcg aagacggccg tgtagccgcc
     2881 gagggcggcc gcggcggacc cggtggcgac gtcctcggac tcctcgccgc cgggctcccg
     2941 cagatgggtg tgcaggtcga cgaaacctgg cagcaggacg tggccgccgg cgtccagcgt
     3001 ctcgatcccg tcgggaccgg cgagcccgcg gccgatctcg gtgacgatcc cgtcccgcac
     3061 gagcaggtcg acggcgtcct cgccgtacgg ccgggcgttc gtgatcagca gatcggcact
     3121 ggtcgtcgct gctgtccggt gtggtgctct cactcgggtg ctcctgccag gaggtggtag
     3181 aggacggcca tccggacgtg cacgccgttg gacacctgcg ccaggatcgc tgccgatgcg
     3241 ctgtcggcca cgctcggcgc gatctccatg ccgcgcacca tcggacctgg gtgcagcacc
     3301 acggcgtgtt cgggcaacag ggcggcgcgc tccgtggaca ggccgtagcc gatcgcgtac
     3361 tcgcgggcgg acgggaagaa cgagccgtgc atccgttcgg cctgcacacg cagcatcatc
     3421 accgcgtcca gtgccgggag ctcggcgtcg agctcggtgc cgacacggca cggccactcg
     3481 tcgacgccga cgggcagcag cgtgggtggc gccacgagca ccacctcggc gcccagcgtt
     3541 gcgagcagga agacgttcga gcgggcgacg cggctgtgca gcacgtcccc gacgatcccg
     3601 atccggcgcc cggcgatccc gcccagccgg tcccgcaacg ttgccgcgtc cagcagcgcc
     3661 tgcgtcgggt gctcgtgggt gccgtcgcct gcgttgacga tcgaggtgta cgtgccggtc
     3721 gtcgcctggt cacggtcgat ccagcccgcg agacggtgtg ccgcaccgga ggcgggatgc
     3781 cggacgatca cgcagtcggc gcccatcgcc gagagcgtca gcgacgtgtc gcgcagcgac
     3841 tcccccttgg aaaccgagga gcccttcgcg ctgacgttga cggtgtcggc gctcatccac
     3901 ttgccggcga tctcgaacga cacgcgggtg cgcgtggaat cctcgtagaa catcgtgatc
     3961 atggtgcggc cgcgcagtgt cggcagcttg cggacctcgc ggcccagcag tgcctgcttg
     4021 agccggtcgg cggtgtccag cagacccgtc gccgtggcgg cgtcgaggtc ggtgaccgag
     4081 agcaggtgtt tcatgcgtcc tctcccgcgc gccgcagccg gacgccgtcg ctgccgtcga
     4141 tctcactcag cagcacgagg atctgctcgt cacgggcggt cggcacgttc ttccccacgt
     4201 agtcggcgcg gatgggcagc tcccggtgac cgcggtcaac cagcacggcg agctgcacgg
     4261 ctcgtggacg gccgtggtcg gacaacgcgt cgagtgcggc ccggacggtg cgcccggaca
     4321 tcagcacgtc gtcgacgagc accaccagcg cgtcgtccag cccgccgtcg ggcacggcgg
     4381 tgtcctccag tgcccgggcc ggacggcggc gcaggtcgtc ccggtacagc gtcgggtcga
     4441 cggagccgac cggcggcccg ctgccctcga actcggcgat tgcgtccgcc agtcgccggg
     4501 ccagcacggt gccgcgggtc gggatgccga tcagcacgac tccgctggca cccgcggtct
     4561 tctcgatgat ctggtgcgcg atgcgcgcga tggtgcgggt gacgtcggca gcgctcagca
     4621 actcccgcgc gggagaggtg gcgcctgccg ggtccccgcg gcggtcatcc gccacgagtc
     4681 gctccttccc cgcctcacgg gacgggtctt aaaggaaaac cggtccctag aacgctacca
     4741 gcggggcccc ggacggcccg ccctccagcg cctcggcatc accagcgtca caccgcgtgg
     4801 gatccgccac tgcacacttc acccgcaacc tgccacagcg cggcgatcga actgtcacga
     4861 tgggagaagg cggccacgcg ccctgcgggg acgtaacgcc tggcggggga ccctcgatca
     4921 gccgttcgag tggtctccac gctctacggc cccgggaggg ggcagccaac ttgaccgatc
     4981 ggccgcgcgc gcgcatgatt actctccgta tcgattgatt gctcccaggg tggccgcggt
     5041 agcggggggc accacctcgg gacgggagcc ggcgcgcagt tacgcgtcgg aaggcacccg
     5101 caccaccggt atgaattcgg tcccgcggcc gatgaaccag caagtgagga ggtcgccaga
     5161 tgggcgacta cgccaaggcg ctcggcggca agctccgggc tatccggcag cagcaggggc
     5221 tgtcgctgca cggggtcgag cagaagtcgg gcggtcgctg gaaggcggtc gtggtgggct
     5281 cctacgagcg cggcgaccgc gcggtcaccg tgcagaagct ggccgaactg gccgacttct
     5341 acggcgtgcc cgtcgccgag ctgcttcccg agggccgcgt cccctccggt tcggagccgg
     5401 ccacgaagat cgtcatcaac ctggagcggc tgcagcagct cccggcggac aaggtcggcc
     5461 cgctggcccg gtatgccgcc gccatccaga gccagcgcgg tgactacaac ggcaaggtgc
     5521 tctcgatccg cgccgaggac ctgcggtcgc tgtcgatcat ctacgacatg acaccgggcg
     5581 agctcaccga ccagctcatc gagtggggcg tgctccctcc cgaggcccgg cccagcgcgt
     5641 agcagcctcg accgcgaggt cgaggcctgc ccgcgaccgc caggccggca ctcctggagt
     5701 gcccggcctg gctgcagtcg tgcgtgtgtc agcgcgtggc gcgtaggtgt tcggcgatgt
     5761 cggcgatgcg gcccaacacc ccgttgaggt accgcgggct gtcgtccgtg gagagcgtgc
     5821 gggccagctc gacggcctcc gtgatcgcca ccgggtcatc gatctcatcc acccacagca
     5881 gctcgaacac gccgatccgc agcagcgtgc ggtcgaccgc gggcatccgc gcgaccgtcc
     5941 agccttcggc gtgctcggcg agcagctggt cgatgcgctc ccggtgctcg acgacgccat
     6001 ccacgagcat cgccgcatag tcggagaccg ggggtgcgtc gtccgtctcg cggcgctggg
     6061 tcagcaccgt gagcgggtcg tcgccgcgtg cctcggactc gaagaggatg tcgagcgcgc
     6121 gcttacgtgc cttcgtccgt gctctcagca tggggatcga ccgagcatca gccggatacg
     6181 cggccgaggt aacgcccgtc gcgggtgtcc accttgacct tctcgccggt ggtgaggaac
     6241 agcggcacct ggatctccgc acccgtctcc accgtggcgg gcttggtgcc gccggtggac
     6301 cggtcgccct gcaggccggg gtcggtgtgg gagatcacga gctccacgga cgtcggcagc
     6361 tcgacgtaca gcggcacccc gtcgtgcagc gcgaccatcg ccgtctggtt ctccagcagg
     6421 tagttcgcgg catcgccgac cgtgccctcc gggatcggga tctggtcgaa ggtgtcgctg
     6481 tccatgaaga cgaagtcggc gccgtcgcgg tagaggaacg tcatgtcccg gcgatcgacg
     6541 gtggcggtgt cgaccttcgt gcccgcgttg aaggtcttgt ccacgacctt gccggtcagc
     6601 acgttcttca gcgtggtgcg cacgaatgcg ccccccttgc cgggcttcac gtgctggaac
     6661 gccgtgaccg accacagctg gccgtccagg ttgagcacca agccgttctt caggtcgttc
     6721 gtggtggcca cgtgcgagct tctcctgcgt ctctactggc tgcgtcgatc ggccgggaga
     6781 ctgccgcggt gcggcaccgc cgtcatgcgc gccggggggt gttctcagtg atcggcaccg
     6841 gatcggcgcc gccgttggct tcacgatacc gctacacctc cacgagctcc ttgctggtgc
     6901 acgtcaacag gtccggcgcg ccgtcgcgca cgacgagggt gtcctcgatg cgcacaccac
     6961 cccgcccctc caggtacacc cctggctcga cggtcaccac catccgggcg gcgagtaccc
     7021 cggtagcggt cttcgacaac gcgggtgcct cgtgaatctg caggccgaca ccgtggccga
     7081 ggccgtgcat gaagtccggg ccccgcccgg cggcgtcgac cacccgccgc gccgcgccgt
     7141 ccacttcgga cacctccgcc ccgggggcga gggcggcccg gcccgcggcc tgggccgccg
     7201 cgaccagggc gtgcagctcc cgctgccatg ccgcggcggg tccgagcacc acggtgcggg
     7261 tcatgtccga gtgatagccg tcgacgagcg ccccgaagtc cattttgacg aggtcaccgg
     7321 cgcgcagctc ggcctcggtc gggctgtggt gcggcaccgc ggagtgggcg cccgccgcca
     7381 cgatcgtctc gaaggaggga ccggcagccc cgtgtctccg catccggttc tccaggtcga
     7441 gcgcgacctc gcgttcggtg cggcccgggc gcaggccacc cgcggcgagc aggtcggcga
     7501 gggcggcatc cgccgcggcg caggcggcgc gcacggcggc gatctccacg tcgtccttca
     7561 ccacccgtag ctgctgcacc agcccgggcg cgcggtgcag cactacgccg tcggccgcct
     7621 cagccatggc cgcgtgcgcg tcgaccgtga cggcgtcaga ctcgaacccg agccgccgca
     7681 caccccaact gcgggccgaa caggcgagcg cgagagcact accccgctcg atgacggtct
     7741 gcaggtcggg cacctgctcc gcggcctgcg tccggtagcg cccgtcggtg cagaagcggg
     7801 tggaggtgtc gccgtcggcg tgcaccagca gcgcggcgtt cgagcccgtg aagccggtca
     7861 ggtaccggat gttgatcagg tcggtgacca tcagggcgtc gacctccgcg gctcggagca
     7921 gcccgcgcag ggcggtacgg cgggaccgga acatgacccg caccgtacgg gaacccgatt
     7981 gtcgttctcg cgccgttctc ccgaccagcg cctcctcccg gcggcctgac cgccgtccgc
     8041 gcagagtccg tgtgctccac gcagggcccc cggaccgggc ggggtccacc cggatgtgtc
     8101 cgccgatcac gtaccgggta gatcacccca tgagaatcgg atcgagcatc ggcctgatcg
     8161 cgatcggcct gatcctcgcg ctcgccatca acgtcaacct gagcgggatc gacctcaccc
     8221 tcgtcgggtg gatcctcgca gccgtcggcg tcgtcgggct gatcgccagc ctggcgctgg
     8281 cccgacggaa ccgccccgtc gtcgtggctc gcgaggagta cccgccccca caacagcgcg
     8341 gctactgatc cggacggcta ctgatcgggg cgggcctgct cggcgatcca gcgcaacgcc
     8401 agaccgtagc cacccatgcc gagcccgacg atcacgccgc tggccaccgc tgagatgtac
     8461 gagtggtggc ggaactcctc gcgagcgtgc acgttactga tgtgcacctc caccaggggc
     8521 gcggtgagct gggcgcacgc gtcgcgcacc gccaccgagg tgtgcgtcca cgccgcagca
     8581 ttgagcacca ccggcgtgga ggcgtcggcg gccccgtgca gccagccaag cagctcgccc
     8641 tcgtggtcgg tctgccggac ctcaaccgcc aggccgagct cacggccggt gcgctcgcac
     8701 agagccacga ggtcggcgta ggtcgtcgcc ccgtacacgg ccggctcccg ggtgccgagc
     8761 ctgccgaggt tcggcccgtt cagcacgagc acgggctgcg gctcgctcac tgggtctcgc
     8821 tcactgggtc tcgctcactg ggacaccgcc gcgtacgccg tctccagcag ctccggcgcc
     8881 ggcccctcca ggcggctagg tgccgccagc ccgtccagca cgacgaagcg cagcatcccg
     8941 gcgcgcgatt tcttgtcccc gcgcatcgtc tgcaccagct ccgggagcgc cccgcgctcg
     9001 taggagaccg gcagcccgag cgactgcagg atcgcgcggt gccgggccgc ggtggcctcg
     9061 tcgaggcgac ccgccgcacg ggccagctcc gcggcgaaca ccaacccgac gctcaccgcg
     9121 gccccgtgcc gccaacggta gtcctcacgt cgctcgatgg cgtgggccag cgtgtggccg
     9181 tagttcagga tctcccgcag gtgtgactcc cgcaggtcgg ccgccaccac gtcggccttc
     9241 acctggatcg agcggcggac gagctcgggg agcacgacgc ccgccgggtc cagcgcagcc
     9301 gcaggatcgg cctcgaacag gtcaaggatc accggatcgg cgatgaaccc ggccttgacg
     9361 atctccgcgc tgccggcggc cagctcggcg gcgggcagcg tgtcgagcac ggccaggtcc
     9421 acgagcaccg ccgacggctc gtggaacgca cccacgaggt tcttgccggc cgccgtgttg
     9481 atgccggtct tgcctcccac tgccgcgtcg accatggcga ggagcgtggt ggggacgtgc
     9541 accacccgca ccccgcgcat ccaggtggcc gccacgaaac cggcgaggtc ggtggccgcc
     9601 ccgccgccga gtgagatcac cacgtcgccg cgggtgaggc cgatccggcc gcagacctcc
     9661 cagcagaacc cggccacggt gagcgccttg ccgtcctctg cgtctggcag ctccacgcga
     9721 tgggcgtcga cgccggcgtc gacgagatcg cagc
//
LOCUS       DASRSC010000134         3795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1519731,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000134 DASRSC010000000
VERSION     DASRSC010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519731"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VFE39_06395"
     CDS             <1..621
                     /locus_tag="VFE39_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6258263.1"
                     /translation="RRDPDAVVPMRLLRGRTFGVMNTVNVMFGAAALGFGTLVPIYAE
                     SRYGIVPLAAGGLLAIRAIGMISLSSLSVFLLRRTGHRILMVAGFALMIVGLVLLALP
                     PQGLAAQAWLSLAAGITGLGMGMAAPASNNASLHLAPREIASITGLRGMFRQSGAIVS
                     ISVTTALLSVSTEPGITQAFVFAVFAVILLAAVPLILSMPDHRGSW"
     gene            658..1446
                     /locus_tag="VFE39_06400"
     CDS             658..1446
                     /locus_tag="VFE39_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HET6258264.1"
                     /translation="MDTVGGVLIDIDGVLTVSWTPLPGAVEAVTTIREAGIPLALVTN
                     TTSRTRRRIAETLTDAGFPIGPDDVLTAPAATAAHLREHRPGTACLLLNSGDVAEDLE
                     GVRLVHGPDARPDVVVVGGAGPEFDYAALNDVFGHLQRGAELLAMHRNLFWRTDAGLQ
                     LDSGAFLLGLEQAAGTEAVVLGKPAEEFFAAALRSLGVTASEAVMVGDDVESDVLGAQ
                     THGLTGVLVRTGKFRPDAEDGVDGHRPQHVVDSIADVPGLLGLT"
     gene            complement(1727..2506)
                     /locus_tag="VFE39_06405"
     CDS             complement(1727..2506)
                     /locus_tag="VFE39_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HET6258265.1"
                     /translation="MAAVPRFVDLTAPQVAALPADTVAVLPLGAVEQHGPHLPVSTDY
                     VTATEAAEAAVAAVAQEGSAPVVLLPGLAYTKSDEHHWSPGTIWLSWDTLMRTLVDIG
                     RSLQSSGITRLLFVNGHGGNSALGQVACREIRRQFGLRTFFAHLSVPVDQGGSGSAAS
                     EHGMGIHGGHGETSLMLHLRPELVHLELAGRRVPEGLREFDLIGFGKPVSFGWTSDDF
                     GPDGYIGDPTGATAEHGKLLFELAVARLAAVIVEASRFTTR"
     gene            complement(2506..>3795)
                     /locus_tag="VFE39_06410"
     CDS             complement(2506..>3795)
                     /locus_tag="VFE39_06410"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6258266.1"
                     /translation="GVGSVSADAVLIMKVSSDWSRMSPVLQEKVPPGRYLADVVVRPA
                     LPEHVPAVLKVAFEHDVPVTPRGAGTGNYGQATPFDGGILLDLRGLDTIAVNPSGTVT
                     AGAGARMTAMDKVARAAGRDIWIFPSTKSSTIGGFVGGGSAGTGTIEKGTTSDGYVVA
                     ATVAPMDGSGTMATVRGEDLTPYIHTYGVTGILVDVEVRTDPARDWAAVYAAFPSYEE
                     LVAVHRTLLDLPQLPRLASADEPALVPTLPAPVALDPARYSLRVIAEASTVDELARRV
                     HSGGGEVVAVLADYTETDRLSGMSYNHPVWFLQRAHPDRSWFHMETGGAPLWDHPDAV
                     RAVYDGPVHLHLELFRHGPGAMVVAGYRGEEAVLAGIPELEGLGIGVHSPHQWYVDRN
                     VGLAIETARRTDPKGLLNPGKLTSTPPVDTRVNIGVR"
BASE COUNT          516 a         1401 c         1326 g          552 t
ORIGIN      
        1 cggcgggatc ccgatgccgt ggttccgatg cggctcctgc gcggtcgcac cttcggcgtg
       61 atgaacacgg tcaacgtgat gttcggcgcg gccgcgctcg ggttcggcac cctggtgccg
      121 atctacgccg agagccgcta cggcatcgtc ccgctcgccg cgggcggcct gctcgcgatc
      181 cgtgcgatcg gcatgatcag cctgtcgtcg ctgtcggtgt tcctgctgcg ccgcaccggt
      241 caccgtatcc tcatggtcgc cggcttcgcc ctgatgatcg tcgggctggt gctgctggca
      301 ctccctcctc agggcctcgc ggcgcaggcg tggctctcac tcgccgccgg gatcaccggc
      361 ctcggcatgg gcatggcggc accggcgagc aacaacgcct cgctgcacct cgccccccgc
      421 gagatcgcct cgatcaccgg gctacgcggg atgttccgcc agtccggcgc gatcgtgtct
      481 atctcggtca ccacggcgct gctgtcggtc agcacggagc ccgggatcac gcaggccttc
      541 gtcttcgccg tattcgccgt catcctgctg gccgccgtcc cgctcatcct ctcgatgccc
      601 gaccaccgcg gcagctggtg acctgatccc tgccactccc gcaggtaacg tgccgccatg
      661 gacaccgtgg gcggggtgct gatcgacatc gacggcgtgc tgacggtctc ctggacgccg
      721 cttcccggtg ccgtcgaggc cgtgaccacc atccgcgagg ccgggattcc ccttgccctg
      781 gtcaccaaca ccacgtcccg gacgcgccgc cggatcgccg agacgctcac cgacgccggc
      841 ttcccgatcg gcccggacga cgtcctcacc gcgcccgctg ccacggccgc acacctgcgc
      901 gagcatcgcc ccggcaccgc atgcctgctg ctgaacagcg gagacgtcgc cgaggacctc
      961 gagggcgtgc ggctggtcca cggacccgac gcgcggcccg acgtggtcgt ggtgggtgga
     1021 gccggcccgg agttcgacta tgcggcactc aacgacgtgt tcggccacct gcagcggggc
     1081 gcggagctgc tcgccatgca ccgcaacctc ttctggcgca ccgacgccgg gttgcagctc
     1141 gacagcgggg ccttcctcct gggcctggag caggcggccg gcaccgaggc ggtggtgctc
     1201 ggcaagcccg ccgaggagtt cttcgccgcg gcgctgcgca gcctcggcgt caccgcctcg
     1261 gaggcggtga tggtcggcga cgacgtggag tccgacgtgc tgggcgcgca gacccacggg
     1321 ctcaccggtg tgctggtgcg caccggcaag ttccggccgg acgccgagga cggcgtcgac
     1381 gggcaccgtc cgcagcacgt cgtcgactcg atcgccgacg tgcccggcct cctcggcctc
     1441 acctgaaccg ggcctgtcac cgaggttcgt cgcagcgccg gagacctcgt cgaggcgtcc
     1501 gagctctcgc tcggtgaggt cgagctccat cgcgacggcg ctctcctgcg cctggcgtgg
     1561 gctgtgaggc gcccggggat cgccacaacg gtgtcgccgt agaagcggac gacccagctg
     1621 agcgccacct gcccgaccga cgcgccgtgc gcccggccga tcgcgcccag ctcgtcgatc
     1681 agcccgtgcg aggggcgcga tcgtgcccgg cccgtcgacc agtgactcac ctggtcgtga
     1741 accgcgacgc ctcgacgatc accgcggcga gccgcgcgac ggcaagctcg aagagcagct
     1801 tgccgtgctc cgcggtcgca cccgtcgggt caccgatgta accgtccggg ccgaagtcat
     1861 cgctcgtcca gccgaacgac accggcttgc cgaacccgat gagatcgaac tcgcgcagcc
     1921 cctccggcac ccggcgcccg gccagctcca gatgtaccag ctcggggcgc aggtgcagca
     1981 tgagcgaggt ctcgccgtgg ccgccgtgga tccccatccc gtgctcggac gcggccgatc
     2041 cgctgccgcc ctggtcgacg ggcaccgaca ggtgcgcgaa gaacgtccgg agcccgaact
     2101 gcctgcggat ctcccggcat gcgacctggc cgagcgcgga gttgccgccg tgcccgttga
     2161 cgaacagcag gcgggtgatg cccgagctct gcaacgaccg tccgatgtcg accagagtgc
     2221 gcatgagcgt gtcccacgac agccagatgg tgcccgggga ccagtggtgc tcgtcggact
     2281 tggtgtaggc gagcccggga agcagcacga ccggcgcgga gccctcctgt gccaccgctg
     2341 cgacggccgc ctccgccgcc tccgtggcgg tgacgtagtc cgtggagacc ggcaggtgcg
     2401 ggccgtgctg ctcgacggcc cccagcggga gcacggccac cgtgtcggcg ggcagcgccg
     2461 cgacctgcgg ggcggtcagg tcgacgaacc ggggaaccgc tgccatcagc gcactccgat
     2521 gttgacgcgg gtatcgacgg gcggcgtcga cgtcagcttg ccggggttga gcagcccctt
     2581 gggatcggtg cggcgggccg tctcgattgc gagcccgacg ttgcggtcca cataccactg
     2641 gtgcggcgag tgcaccccga tccccagccc ctccagctcc gggatgcccg ccagcaccgc
     2701 ctcctcgcca cggtatccgg cgacgaccat cgcccccggc ccgtggcgga acagctccag
     2761 gtgcaggtgc accggcccgt cgtagacggc gcggacggcg tcggggtggt cccagagcgg
     2821 tgcgccgccg gtctccatgt ggaaccagct ccggtcggga tgggcccgct gcaggaacca
     2881 gaccgggtgg ttgtaggaca tgccggagag ccggtcggtc tcggtgtagt cggcgagcac
     2941 cgccacgacc tcccccccgc cgctgtggac ccggcgggcg agctcgtcga ccgtcgaggc
     3001 ctcggcgatg acgcgcaggc tgtaccgggc cggatctagg gccaccggag cgggcagcgt
     3061 cgggacgaga gcgggctcgt cggccgaggc gagccggggc agctgcggca ggtcgagcag
     3121 cgtgcggtgc acggcgacga gctcctcata ggacgggaat gccgcgtaca ccgccgccca
     3181 gtcgcgcgcg ggatcggtac ggacctcgac gtcgaccagg attcccgtga ccccgtaggt
     3241 gtggatgtag ggcgtcaggt cctcgccgcg caccgtggcc atcgtgccgc tgccgtccat
     3301 cggcgcgact gtcgccgcga cgacgtaacc gtcgctcgtc gtgcccttct cgatcgtacc
     3361 ggtgcctgcg ctcccaccgc cgacgaaccc gccgatcgtc gagctcttcg tggacgggaa
     3421 gatccagatg tcgcgaccgg cagcgcgcgc caccttgtcc atcgccgtca tccgtgcacc
     3481 cgcccctgcg gtgacggttc ccgaggggtt gacggcgatc gtgtcgagac cgcgcaggtc
     3541 gagcaggatc ccgccgtcga acggcgtggc ctgcccgtag ttgcccgtgc ccgcgccgcg
     3601 cggcgtcacc gggacgtcgt gctcgaaggc gaccttcagc acggccggga cgtgctcggg
     3661 cagtgcgggg cggacgacga cgtcggcgag gtagcgcccc ggcggcacct tctcctgcag
     3721 gaccggcgac atccgcgacc agtccgagga gaccttcatg atcaacacgg cgtcagcgga
     3781 gacggacccc acgcc
//
LOCUS       DASRSC010000135        11632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1519896,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000135 DASRSC010000000
VERSION     DASRSC010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11632
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1519896"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VFE39_06415"
     CDS             complement(<1..466)
                     /locus_tag="VFE39_06415"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6258267.1"
                     /translation="MATWNLLHGLDVRTGRIDLGAVAAAIDALDVDVLAVQEVDREQR
                     RSGRVDQMADLAGRLGWHSVFAPALLGEPARRWHAAPGDGTDPGGPAYGIGLLSRHPL
                     LCPERTTLCGGDAGERRPEDAGRALPGWDHEPRTVLRAQVVPPGDRPLVVATA"
     gene            complement(469..1014)
                     /locus_tag="VFE39_06420"
     CDS             complement(469..1014)
                     /locus_tag="VFE39_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6258268.1"
                     /translation="MARTSQPRRTTITLDPRIGSYDHLHGVLGAELTLLEYGDFECPY
                     CRKAAPVIEEVRRRLGDRLLFAFRHFPLAELHPYALSAAVASEAAALKGQFWPMHERL
                     FSGDEPRLTQDDLRRYAKEIGVPPEKVVWPATQFVEDRVEADFNSGVRSGVRGTPTFF
                     VNGVQYRGAVSVRDLIDALEQ"
     gene            1099..2388
                     /gene="nhaA"
                     /locus_tag="VFE39_06425"
     CDS             1099..2388
                     /gene="nhaA"
                     /locus_tag="VFE39_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HET6258269.1"
                     /translation="MRTESGSAVLLLTAAVIALIWANLGTGYEDFWHTELSFRLGDAA
                     LTYDLRHWINDGLMVLFFFSVGLEISREMVLGELRGVRALATPALAALGGLTVPAGLF
                     LLFNAGGEAAGAWGIPISTDTAIVIGMLALIGPRCPDQLRVFLLALAIVDDIGAVAAI
                     AFFYTDNVQFTALAVAGVLFLALLALRYVQFWRTPIYVLIAVVMWLAVLRSGVHPSVV
                     GVALGLLVNAYAPRPRDIARVQVLGSSFIVDPSPERALAAQTAVTEAVSPNERLQLRI
                     QPWSSYVIVPIFVLANAGVVLTAATLAAAIASPLFWGIVVGLTVGKLIGVTLGTWVAL
                     RTGIGRVPDTLRWGQIVGGAGLAGIGFTVALFITDLALKDPVLVNDAKIGILTGSILA
                     GVIGWLIFRLAGERGGQCAPSGLPVLPPRPWRPPVEA"
     gene            complement(2420..3763)
                     /locus_tag="VFE39_06430"
     CDS             complement(2420..3763)
                     /locus_tag="VFE39_06430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET6258270.1"
                     /translation="MGARWVLHLDMDAFFASAEQLTRPTLRDRPVLVGGLGPRAVVAG
                     ASYQARVFGARSAMPMGQARRLCPHAVVLPARFALYQALSERVMGVLANAAPVLEPVS
                     LDEAFLEPPVLAGAAVADVERFGTELRAAVRDATGLPASVGAGSGKQLAKIASELAKP
                     DGLRVVAAHEEQAVLAPLPVRALWGVGPVAEAGLRKLGVHTIGELAAMDLRELTGLLG
                     TAIGTELHRLARGLDERPVAPRGAAKQVSAETTFDTDLTAMTAVHDAVARMTEAAHRR
                     LLVSGRAARTVTVKVRSADFSTSSRSETAGSGSTDLDTLTAVAQRLARAAVPEGGVRL
                     IGVSLAGLIDDPPPALFEQARFEAVPAVASVPEPGEPGQQPTDRRAAVDAIARPWGPG
                     DDIRHAEHGHGWVQGAGHGRVTVRFETCSTGPGRARTFAADDPDLVRADPLGSLD"
     gene            complement(3868..4554)
                     /locus_tag="VFE39_06435"
     CDS             complement(3868..4554)
                     /locus_tag="VFE39_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HET6258271.1"
                     /translation="MGDDIGTSVWWAAPVMPDAAPGLVSLLDPHERARLDRFRMAADR
                     ARYLAAHALTRLVLADAVGRSAAELEFDRTCQCGQQHGKPVLLGGPGFSLTHAGDLVG
                     VAVHPRGPVGLDVEEVRDLADLASMAAHVCSPGESVLDVSSFFAVWTRKEALLKATGA
                     GLSSPMAAITLGPSGVREWTGDGAPSEPVWLRDLRPADGYLAALAGLGAEPSHVREAD
                     GNDVLHAEVA"
     gene            complement(4755..5015)
                     /locus_tag="VFE39_06440"
     CDS             complement(4755..5015)
                     /locus_tag="VFE39_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258272.1"
                     /translation="MRDPLLSTVLADTVLADTVLADTLPVDTVPAEPALDPAGAEPAP
                     VNRAARRGRSGKQAPSGALHAGSAGHARAAQGRRINPVRRTG"
     gene            5273..6403
                     /locus_tag="VFE39_06445"
     CDS             5273..6403
                     /locus_tag="VFE39_06445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6258273.1"
                     /translation="MTSSADVVVIGGGIAGASIAYELAATRSVVLLEAETALAKHSTA
                     RSAATYIPGHGTLAVRALIAASGPRFARLAAELDAPPLLAPRAVVNIAVDDVGEARLA
                     AELVEQAGEPGAPVALEPAEAERRCLVLVPGVVRAAAVVEGAADVDAEALHQAYVRGL
                     RRRGGTVRTGAGATGLERRGGGWRVQAGPDTIDTADVVDAAGAWADLVAGLAGVPRVG
                     LTPLRRTIALVRLPDPARLRVPNGRAPMVREAAERFYFKPEGDGLLVSPADETPVEPH
                     DVRPDELDVATALERVEAVTGLGLRSVQTSWAGLRSFVPDRRPVVGAWPDHPGFHFFA
                     GQGGSGIESAPALAAFGAAVVTGEPVPADIAVVPETLRPSRL"
     gene            6440..7630
                     /locus_tag="VFE39_06450"
     CDS             6440..7630
                     /locus_tag="VFE39_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6258274.1"
                     /translation="MTGTLELYEGAFWRDPYPAFSVLRTEAPAHEVHLPDGPAWIITR
                     YADVRSALTDPRFSKDWRYTLPPEARADAPANPLPMMILMDPPGHTRLRKLVSRSFTA
                     RRMAELRPQVRRYAEDLLDALPVGDQVDLMAHYASLLPVRVICELLGIPETDREQFTA
                     WTRTMLDETSQAESQAASGEMSAYFGRLVEVKRAQPDDALLSALVEVSDDGDRLSAEE
                     LVAMAMLLLIAGHETTVNLIGNGTFGLLTHPDQLAMLRERSDLLGAAVEEFLRWDAPV
                     QSPPVRFLSEDVEIGGVTVPAGAIVMLSLAAANRDPEQFPDPDELKIDRDTSGQVAFG
                     YGLHFCLGAQLARIEGQEAFAALLTRFPDISLAVEPEELVHRRSTLVRGLTELPVRLS
                     PAPA"
     gene            complement(7698..8324)
                     /locus_tag="VFE39_06455"
     CDS             complement(7698..8324)
                     /locus_tag="VFE39_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HET6258275.1"
                     /translation="MTRTREQLAARVAAELPDGSYVNLGIGMPTLVPGFIPVGRTVVL
                     HSENGILGVGPYPTEDEVDPDLINAGKETVTVLPGASYFDSATSFGMIRGGHVDVAVL
                     GAMQVSARGDLANWAVPGKMIKGMGGAMDLVHGARRVIVMMEHTARDGSPKIVQECAL
                     PLTGAACVDRIITDLAVIDVTDDGLALVETAPGVTTDEVRAATGARLR"
     gene            complement(8356..9111)
                     /locus_tag="VFE39_06460"
     CDS             complement(8356..9111)
                     /locus_tag="VFE39_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HET6258276.1"
                     /translation="MDKVVASAAEAVADVRDGASLAVGGFGMSGVPAALIAALLEQGA
                     TDLETISNNLGIDGFGLGTLLAAGRIRRTIGSYVGNNKEFARQYLAGEIELELTPQGT
                     LAERLRAGGAGIPAFFTPAGVGTQVADGGLPWRHDAQGGVAVRSPRKEVREFGGRSYV
                     LEEAIVPDFALVHAWMGDRHGNLVYRRSSRNFNPVCAAAGRITIAQVEHLVEPGEIDP
                     DAVHTTGIQVQRVVHVPDADKPVEFRTVRTPGA"
     gene            9460..10587
                     /gene="pcaB"
                     /locus_tag="VFE39_06465"
     CDS             9460..10587
                     /gene="pcaB"
                     /locus_tag="VFE39_06465"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HET6258277.1"
                     /translation="MSSALFDPVFGADRVAARLDDASWVAALVAVEVALSRAAAEHGL
                     VPVADAAHIAAVAADLQVDPSELGQAAVEGGNPVIPLVRLLREAAGDAGRSVHPGATS
                     QDVMDTAAVLLSGWAGEIVLDDLRGAAEAAAALAAAHRDTPMIARTLGQQALPTTFGL
                     VAAGWCAGLDRGRTELARVLAALPVQFGGAAGTLAALHPHGLAVAASLARGLGLADAA
                     PWHTERTRMGELAGALAVAAGACAKPATDIVLLAGTELGEVSEAAPGDSSSMPHKRNP
                     IAAITARGAARRAPGLAAVLLAAADHEHQRAAGAWHAEWQTLGELLRATGGAAARLRT
                     SLGGLIVHPDRMAANLTLTGGTDASPGHAAELVERLLMERP"
     gene            10658..11485
                     /locus_tag="VFE39_06470"
     CDS             10658..11485
                     /locus_tag="VFE39_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HET6258278.1"
                     /translation="MTEHVRSLARGLAVIRAFDAERPEQTLSEVARRTGLPRAATRRF
                     LLTLVDLGYVRTDGRLFALTPRVLELGYAYLSSLSLPEIAAPHLEALVRQVQESASVS
                     VLDGADVVYVARVPTSRIMTVAITIGTRFPAYATSMGRVMLAALPPAERSARLATLRP
                     QPLTDRTLTDPAALGQELDVVRDAGFCVVDQELELGLRSISVPVRDAGGTVIAAVNVS
                     TRAGSRDVVRDVLPELRACAAAIEADLAAGTGAAGPTGVPQPESSGARAVVAQPSSP"
BASE COUNT         1500 a         4212 c         4303 g         1617 t
ORIGIN      
        1 gcgcggtggc gacgacgagg ggccggtcgc ccggtggaac cacctgcgcc cgcagcaccg
       61 tccgtggttc gtggtcccaa cctggcagcg cgcgtccggc gtcctcggga cgtcgctcgc
      121 ccgcgtcgcc cccgcagagc gtggtgcgtt cagggcagag cagcggatgc ctgctcagca
      181 gcccgatccc gtacgcggga ccgccggggt ccgtgccatc tcccggggcg gcgtgccacc
      241 gcctggccgg ctcgccgagc agggcgggcg cgaacacgct gtgccagccg agcctgccgg
      301 caaggtcggc catctggtcc acccggccgc tgcgccgctg ctcacggtcg acctcctgca
      361 ctgccagcac atcgacgtcg agcgcgtcga tcgctgcggc gacggcgccg agatcgatcc
      421 ggccggtgcg cacatccagc ccgtgcagca ggttccaggt cgccactctc actgctcgag
      481 agcgtcgatg aggtccctca ccgagacagc cccgcggtac tgcacgccgt tgacgaagaa
      541 cgtcggggtg ccccgtacgc cgctgcggac cccggagttg aagtcggcct ccacccggtc
      601 ctcgacgaac tgggtggcgg gccacaccac cttctccggg ggaacgccga tctccttggc
      661 gtagcggcgc aggtcgtcct gggtcagcct gggctcgtca ccggagaaga gccgctcatg
      721 catgggccag aactggccct tgagcgccgc ggcctcggac gcgacggcgg ccgacagcgc
      781 gtacgggtgc agctcggcaa gcgggaagtg gcggaacgcg aagaggaggc ggtcgccgag
      841 gcgccgccgc acctcctcga tcaccggggc cgccttgcgg cagtacgggc actcgaaatc
      901 gccgtactcg aggagtgtga gctccgcgcc gagcacgccg tgcagatggt cgtagctccc
      961 gatccggggg tcgagcgtga tcgtggtcct cctgggctgc gacgtccggg ccagggacgt
     1021 ccgactggca ggatctgctc cgtggcggtg acaaccctaa ccgagcgcgg cgacctgagt
     1081 cgcgctgcgc ggtccttcat gcggaccgaa tccggctcgg cggtgctgtt gctcactgct
     1141 gcagtgatcg cactcatctg ggcgaacctc gggacgggat acgaggactt ctggcacacc
     1201 gagctgtcgt tccgcctcgg tgacgccgca ctcacctacg acctgcggca ctggatcaac
     1261 gacggactga tggtgctgtt cttcttcagc gtcgggctgg agatctcccg cgaaatggtg
     1321 ctcggtgagc tgcgcggggt ccgcgccctc gccacgcccg cactggccgc actcggcggg
     1381 ctgaccgtgc cggctgggct cttcctgctg ttcaacgcgg gcggggaggc cgcgggagcc
     1441 tggggtatcc cgatctccac cgacaccgcc atcgtgatcg gcatgctcgc gctgatcggg
     1501 ccccgctgcc ccgatcagct gcgggtgttc ctgctcgcgc tcgcgatcgt cgacgacatc
     1561 ggcgctgtcg ccgccatcgc gttcttctac accgacaacg tgcagttcac cgccctggcc
     1621 gtggcgggag tgctgttcct cgcgttgctc gcgctgcggt acgtccagtt ctggcgcacc
     1681 ccgatctatg tgctcatcgc ggtggtgatg tggctggcgg tgctgcggtc cggcgtgcac
     1741 ccgagcgtcg tcggggtggc gctgggcctg ctcgtcaacg cctacgcacc gcgcccgcgc
     1801 gacatagcga gggtccaggt gctgggcagc agcttcatcg tcgacccgag ccccgaacgg
     1861 gcgctcgcgg cacagacggc ggtgaccgag gccgtttcgc cgaacgagcg gctgcagctg
     1921 cgcatccagc cgtggagcag ctacgtcatc gtcccgatct tcgtgctggc caacgcgggc
     1981 gtggtgctca cggctgccac cctcgccgcc gccatcgcct caccgctctt ctggggcatc
     2041 gtcgtggggc tgacggtcgg caaactgatc ggcgtcaccc tgggcacctg ggtggcgctg
     2101 cgcaccggga tcggccgggt gcccgacacg ctgcggtggg ggcagatcgt cggaggtgcc
     2161 ggactcgcgg gtatcgggtt cacggtggcg ctgttcatca ccgacctggc cctcaaggac
     2221 ccggttctcg tgaacgacgc gaagatcggc atcctgaccg gctcgatcct ggcgggtgtg
     2281 atcggctggc tgatcttccg gctggcgggg gagcgcggcg gccagtgcgc gccgagcggg
     2341 cttccggtgt tgccgccgcg cccgtggcgg ccgccggtgg aggcttgatc aagccccgat
     2401 catctgacca tcctcacagt cagtccaggc tccccagcgg gtcggcgcgg acgaggtcgg
     2461 gatcgtccgc ggcgaaggtg cgcgctcgcc ctgggccggt gctgcaggtc tcgaaccgga
     2521 cggtgactcg gccgtgcccg gccccctgca cccagccatg accgtgctcc gcgtgtcgta
     2581 tgtcgtcgcc ggggccccac gggcgcgcga tcgcgtccac cgctgcacgg cggtccgtcg
     2641 gctgctgccc cggttcgccg ggctcgggca ccgacgctac cgccggtacc gcctcgaacc
     2701 gggcctgctc gaacagcgcg ggaggcggat cgtcgatgag cccggccagc gacaccccga
     2761 tcaaccggac cccaccctcg ggcacggcgg cccgcgccag gcgctgtgcc acagcggtga
     2821 gcgtgtcgag gtcggtgctc ccgctgcctg cggtctccga gcggctcgac gtggagaagt
     2881 cggcgctgcg caccttgacc gtgacggtgc gcgccgcccg tcccgacacc aggagccgcc
     2941 ggtgcgccgc ctccgtcatc ctggcgacgg cgtcgtgcac ggcggtcatc gcggtgaggt
     3001 cggtgtcgaa cgtggtctcc gcgctcacct gcttcgccgc gccccgcggc gccaccggcc
     3061 gctcgtccag gccgcgggcg aggcgatgca gctcggtgcc gattgcggtg cccagcagcc
     3121 cggtgagctc ccgcaggtcc atggcggcca gctcgccgat ggtgtgcacg ccgagcttgc
     3181 gcagcccggc ctcggccacc ggcccgacgc cccacagggc gcgcaccgga agcggcgcga
     3241 gaaccgcctg ctcctcgtgc gccgccacca cgcgcagccc gtcgggcttc gcgagctcgg
     3301 acgcgatctt ggccagctgc ttgcctgagc cggctcccac ggaggcgggg agcccggtgg
     3361 catcgcgcac ggcggcccgc agctccgtgc cgaaccgctc gacatcggcc accgctgctc
     3421 ccgcgagcac agggggctcc aggaacgcct cgtccagcga caccggctcg agcaccggcg
     3481 cagcgttggc gagcacgccc atcacccgct cgctcagcgc ctggtacaga gcgaaccgcg
     3541 ccggcagcac cacggcgtgc gggcacaatc ggcgggcctg gcccatcggc atcgccgagc
     3601 gggctccgaa cacacgcgcc tggtagctgg ccccagccac gacggcgcgc ggcccgagcc
     3661 cgcccaccag caccggccgg tcgcggaggg tgggacgggt gagctgctcg gcggaggcga
     3721 agaacgcgtc catgtcgagg tgcagcaccc accgcgcgcc cactccgcca tcatccctga
     3781 gcccactggc agtctcccgc agcggcgctc cgctccaccc gacggcgcga cgagggcgct
     3841 gcgatctgga gcggcgctac ccccggctca ggccacctcg gcgtgcaaca cgtcgttgcc
     3901 gtcggcttca cggacgtgcg acggctcggc accgagcccg gcgagcgcgg ccaggtagcc
     3961 gtcggcggga cgcaggtcgc gcagccacac cggctcggac ggcgctccgt cgcccgtcca
     4021 ctcccgcacg cccgacggcc ccagcgtgat cgccgccatc ggactcgaca atccggcgcc
     4081 ggtggccttg agcagcgctt ccttgcgcgt ccacaccgcg aagaaggacg acacgtcgag
     4141 cacggactcg ccgggcgagc agacgtgcgc ggccatcgag gcaaggtcgg cgaggtcgcg
     4201 aacctcttcg acgtcgaggc cgaccgggcc cctcggatgc acggccacgc cgacgagatc
     4261 tccggcgtgg gtgagcgaga atccggggcc gccgaggagc acgggcttac cgtgctgctg
     4321 cccgcactgg caggtgcggt cgaactcgag ctccgcggcg gaacgcccga cggcgtcggc
     4381 cagcacgagc cgggtgagcg cgtgcgcggc gaggtagcgc gcgcggtcgg cggccatccg
     4441 gaagcggtcg agccgggccc gctcgtgcgg gtccagcagc gagacgaggc cgggtgcggc
     4501 gtccggcatg acgggcgcgg cccaccagac gctggtcccg atgtcgtcgc ccacaccacc
     4561 atggtcacac gtgccggcac agccgtcccc ggccgccgga cgggaccacg tccgccacat
     4621 ggacacgtgt cgtggattga ggccgctgcg gaacagcgag atgtgtccat gtcgcggccg
     4681 agccgggccg gggtcaggct ggagcgaggt accgccggcc ccgtggacgc gaggccggcg
     4741 gtaccgaggg cggatcagcc ggtgcgacgc accgggttga tccggcggcc ctgagccgcc
     4801 cgggcgtggc cggcggagcc ggcatgcagg gctccgctcg gtgcctgctt gcccgagcgg
     4861 ccgcgacggg ccgcgcggtt gaccggcgcc ggttcggctc cggccgggtc gagagccggc
     4921 tcggcgggca ccgtgtctac gggcagggtg tcggcgagga cggtgtcggc cagaacggtg
     4981 tcggcgagga cggtggacag caacggatca cgcatgagtg cctcctgcag gaaggacgag
     5041 gccggcacac cgatgccggg aaagggcccg gatctcatcc agggccggcc ggggtggctg
     5101 ggacgggcgg cgcggacgga ggtccagggc cgcggtgccg cgcgaggcgg tcgtctctac
     5161 aggcgcatgg gaagaaccat aggggggccg ggatcggggt gccacgggtt ttccggcccg
     5221 cgcgtgtccc gcgggccgga tgatccactc gacatgccct agcgtcgcgc acatgaccag
     5281 ctccgcggac gtcgtcgtga tcggtggcgg gatcgcgggc gcctcgatcg cgtatgagct
     5341 cgctgccacc cgttcggtgg tcctgctgga ggcggagacc gcacttgcga agcactcgac
     5401 ggcccgctcc gcggccacgt acatccccgg gcacggcacc ctcgccgtcc gtgccctgat
     5461 cgccgcgagc gggccgaggt tcgcacggct tgccgcggag ctggacgcgc caccgctgct
     5521 cgcgccacgc gcggtcgtga acatcgcggt ggacgacgtc ggcgaggcga ggctcgccgc
     5581 ggagctcgtg gagcaggcgg gggagcccgg cgccccggtg gcgctcgagc cggcggaggc
     5641 ggagcgcagg tgcctggtcc tcgttccggg tgtggtgcgc gccgccgcgg tggtggaggg
     5701 tgcggccgat gtggatgccg aggcgctgca ccaggcctac gtccgggggc tgcggcggcg
     5761 cgggggcacg gtccggaccg gtgcgggtgc cactggactc gagcggcgcg ggggcggctg
     5821 gcgggtgcag gccgggcccg acacgatcga cacggccgac gtcgtggacg ccgcgggtgc
     5881 gtgggccgac ctcgtggccg ggctggccgg cgtcccgcgc gtcgggctca ccccgctgcg
     5941 ccgcacgatc gcgctggtgc gactgcccga cccggcgcgg ctgcgggtgc cgaacggccg
     6001 cgcgccgatg gtccgggagg cggccgagcg gttctacttc aaaccggagg gcgacggatt
     6061 gctggtgagc ccggcggacg agacgcccgt cgagccccac gacgtccggc ccgacgagct
     6121 cgacgtggcg acggcgctgg agcgcgtcga ggcggtgacc gggctcgggc tccgatccgt
     6181 gcagacgagc tgggcaggcc tacgatcgtt cgtcccagat cggcggccgg tggtcggggc
     6241 ctggcccgac cacccggggt tccacttctt tgcaggtcag ggcggctcgg gcatcgagag
     6301 cgccccggcg ctggcggcgt tcggcgccgc ggtggtgacc ggtgagccgg tgccggcgga
     6361 tatcgcggtc gtaccggaaa cgctcagacc gtcgcgactg taagcttccg cttacatccg
     6421 cgatcttcga aggagaacgg tgaccgggac tctggagctc tacgagggcg ccttctggcg
     6481 cgatccctac cccgccttct cggtcctgcg cacggaggcc ccggcccacg aggtacacct
     6541 gccggacggt ccggcgtgga tcatcacgcg ctacgcggac gtccgcagcg cgctcaccga
     6601 cccacggttc agcaaggact ggcgctacac gctcccccct gaggcgcggg ccgacgcacc
     6661 ggccaacccg ctcccgatga tgatcctcat ggacccaccg gggcacacca ggctgcgcaa
     6721 gctcgtgtcg cgctcgttca ccgcccgccg gatggccgag ctgcgcccgc aggtgcggcg
     6781 ctacgcggag gacctgctcg acgcgcttcc ggtcggcgac caggtcgatc tcatggccca
     6841 ctacgcgtcg ctgctgcccg tgcgggtgat ctgcgagctg ctcggcatcc cggagacgga
     6901 ccgcgagcag ttcaccgcgt ggacgcggac gatgctcgac gaaacctcgc aggcggagtc
     6961 acaggcggcg agcggcgaga tgtccgccta cttcgggcgg ctggtcgagg tcaagcgcgc
     7021 gcagccggac gacgcgctgc tcagcgccct cgtcgaggtg agtgacgacg gcgaccggct
     7081 gtcggccgag gagctggtgg cgatggcgat gctgctgctc atcgccggcc acgagaccac
     7141 cgtcaacctc atcggcaacg ggacgttcgg gctgctcacc caccccgatc agctcgccat
     7201 gctgcgcgag cggtccgacc tgctgggcgc ggccgtggag gagttcctgc gctgggacgc
     7261 gccggtgcag agcccgccgg tccggttcct cagcgaggac gtcgagatcg gcggggtcac
     7321 cgtccccgcc ggggcgatcg tgatgctgtc gctggccgcg gccaaccgcg accccgagca
     7381 gttccccgac cccgacgagc tgaagatcga ccgtgacacg agcgggcagg tcgcgttcgg
     7441 ttacggcctg cacttctgcc tcggtgcgca gctggcccgg atcgagggtc aggaggcctt
     7501 cgccgcgctc ctcacccgct tccccgacat ctcgctcgcc gtcgagccgg aggagctggt
     7561 gcaccgccgc agcacgcttg tgcgtggcct caccgagctc ccggtccggc tctcgcccgc
     7621 acccgcctga cggagggcgc agcgccggca ccgcaggaga ggtcggctac cggccctgcg
     7681 tcgtccccgg cggcgcgtca gcgcagtcgc gcgcccgtcg ccgctcgcac ctcgtccgtg
     7741 gtcacgcctg gcgctgtctc gacgagcgcg aggccatcgt ccgtcacgtc gatcacagcc
     7801 aggtcggtga tgatccggtc gacgcacgcc gccccggtga gcggcaatgc gcactcctgc
     7861 acgatcttcg gtgagccgtc gcgtgcggtg tgttccatca tcacgatcac ccggcgggct
     7921 ccgtggacga gatccatcgc cccgcccata cccttgatca tctttccggg aaccgcccag
     7981 ttggcgaggt cgccgcgggc gctgacctgc atcgctccca gtacggcgac gtccacatgc
     8041 cccccgcgga tcatgccgaa gctcgtggcc gagtcgaagt acgacgcccc gggcagcacg
     8101 gtgaccgtct ccttgccggc gttgatgaga tcggggtcga cctcgtcctc ggtcgggtac
     8161 gggccgacgc cgaggatccc gttctcggag tgcagcacga cggtgcggcc cacggggatg
     8221 aagccgggca ccagcgtcgg catgccgatc ccgaggttga cgtaggagcc gtcggggagc
     8281 tcggctgcca ccctcgcggc gagctgctca cgggtgcggg tcatgctgct ctcccaggtg
     8341 agctcggaag ctcggtcatg cgcccggcgt ccggaccgtg cggaactcca cgggcttgtc
     8401 ggcgtcgggc acgtgcacca cccgctgcac ctgaatgccc gttgtgtgca cggcgtcggg
     8461 gtcgatctcc cctggctcga cgaggtgctc cacctgcgcg atggtgatgc gcccggctgc
     8521 cgcgcagacg gggttgaagt tgcgggagct gcgccggtag acgaggttgc cgtgccggtc
     8581 gcccatccag gcgtgcacga gggcgaagtc ggggacgatc gcctcctcca gcacatagga
     8641 ccgcccgccg aactcgcgca cctccttccg cggcgagcgc acggccactc cgccctgggc
     8701 atcgtgccgc cacggcagac cgccgtcggc gacctgggtg ccgacgccgg ccggggtgaa
     8761 gaacgcgggg atgcccgcgc cgcccgcgcg caaccgctcg gcgagcgtgc cctgtggcgt
     8821 gagctccagc tcgatctccc cggccaggta ctggcgggcg aactccttgt tgttgcccac
     8881 gtacgacccg atcgtgcgcc ggatccgacc ggcggcgagc agcgtgccga gcccgaaccc
     8941 gtcgatgccg aggttgttgc tgatcgtctc caggtcggtg gcgccctgct ccagcagtgc
     9001 cgcgatcaac gctgcgggga cgccggacat cccgaacccg cccaccgcga gcgacgcgcc
     9061 gtcgcggacg tcggccaccg cctcggccgc ggacgcgacc accttgtcca tcacgagatc
     9121 cgaccctacg acgcggtgcg ttcctcgcac aatggtgcgc acagcgaaca gaacggcgaa
     9181 cagtccagcg ggggcgggtg ctgctggccg ggccgccgcg tacctggtgc cgccgttcgg
     9241 agcgcgcgga cacgatcatc ggcgtgggac ggagagcgcc cgaattcgcc ggtggctgta
     9301 ccggactggt gatcatggct tgggtgagcc ccgtgggccg tgggccgtgg gtctggccgc
     9361 gccgggcggg tggcgcgtcc cgccgccaca gatcgcgccg ggcagggccg tccggggtct
     9421 tacctgcgcc gggcggcgca ggccgtacgg tctcggccca tgtccagtgc gttgttcgac
     9481 ccggtgttcg gggccgaccg ggtcgcggcc cgcctcgacg acgcttcgtg ggtggcggcg
     9541 ctggtcgccg tcgaggtcgc gctgtcacgc gccgcggccg agcacggcct cgtccccgtt
     9601 gcggacgccg cgcacatcgc agcagtcgcg gccgacctgc aggtcgaccc ctcggagctg
     9661 ggacaggctg cggtggaggg cgggaacccc gtgatcccgc tggtccggct gctgagggag
     9721 gctgcgggag acgccgggcg gtccgtccac ccaggcgcga cgagccagga cgtcatggac
     9781 acggccgcgg tgctgctcag cgggtgggcg ggcgagatcg tgctcgacga cctgcgcggg
     9841 gccgccgagg ccgccgccgc actggctgcc gcgcaccgtg acaccccgat gatcgcccgc
     9901 accctcggcc agcaggcgct cccgacgacg ttcggtctgg tcgcggccgg gtggtgcgcc
     9961 ggtctggacc ggggccgcac cgagctggcg cgcgtcctcg ccgcgctgcc cgtgcagttc
    10021 gggggtgccg caggcaccct cgcggcgctg cacccgcacg gcctcgccgt ggccgcctca
    10081 ctcgcccgcg gcctcgggct ggccgacgcg gcaccgtggc acaccgagcg cacccggatg
    10141 ggcgagctcg caggcgccct ggccgtggcc gcgggggcct gcgcgaagcc ggccacggac
    10201 atcgtgctgc tcgccggcac cgagctcggc gaggtgtccg aggcggcgcc gggcgactcg
    10261 tcgtcgatgc cccacaaacg caacccgatc gctgcgatca cagcgagggg agcggcccgc
    10321 cgggctcccg ggctcgctgc ggtgctgctc gccgccgccg accacgagca ccagcgcgcc
    10381 gcgggggcct ggcacgccga gtggcagacg ctcggcgagc tgctccgcgc cacaggcggc
    10441 gccgcggccc ggctccggac gtccttgggg ggcctcatcg tccaccccga ccggatggct
    10501 gcgaacctca cgctcaccgg cggaacggac gcctcaccgg ggcacgcggc cgagctggtg
    10561 gagcggctgc tcatggagcg gccgtgacgg gggcaggccg gggtgcggcc gcgaggtggg
    10621 tggctgtgca gggggcggcc gtgagggagg tggcggcgtg accgagcacg tccgctcgct
    10681 cgcccggggc ctcgccgtga tcagagcgtt cgacgccgag cgccccgagc agacgctcag
    10741 cgaggtggca cgccgcacgg ggcttccgcg cgccgcgacc cgccggttcc tgctcaccct
    10801 cgtcgacctc ggttacgtcc gcaccgacgg caggctcttc gcgctgaccc cacgtgtgct
    10861 ggagctgggc tacgcctacc tctccagcct gtcgctgccc gagatcgccg cgccgcatct
    10921 ggaggcactg gtccggcagg tgcaggagtc ggcttcggtg tcggtgctcg acggtgcgga
    10981 cgtcgtctac gtcgccaggg tgccgacctc ccggatcatg accgtcgcga tcacgatcgg
    11041 tacccgcttc ccggcctacg ccacgtcgat gggtcgggtg atgctggcgg ccctgccgcc
    11101 cgccgagcgg tcggcccggc tcgccacgct gcgcccgcag cccctgaccg accgcacgct
    11161 caccgacccc gcggcgctcg gccaggagct cgacgtggtg cgcgacgccg ggttctgcgt
    11221 cgtcgaccag gagctggaac tggggctgcg ctcgatctcc gtcccggtgc gtgacgcggg
    11281 aggcaccgtc atcgccgcgg tgaacgtcag caccagggcg ggttcgcgtg acgtcgtgcg
    11341 cgacgtgctc ccggagctgc gggcctgtgc ggccgcgatc gaggccgacc tcgcggcggg
    11401 gaccggggct gcgggaccga cgggtgtgcc gcagccggaa tcgtccggcg ctcgggcggt
    11461 ggtggcgcag ccatcctcgc cctgatgggt aatgggccct gacgggtaat gggccctgat
    11521 cggggaatgg gcccggatga ggaatgggaa tgccgtggct gcggcgccgg gttgcatgag
    11581 ggacgggcga ggcgagccac cggagggcac atatgggacg gggcgcgtac ct
//
LOCUS       DASRSC010000136        24833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_864218,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000136 DASRSC010000000
VERSION     DASRSC010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 24833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24833
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864218"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..362)
                     /locus_tag="VFE39_06475"
     CDS             complement(12..362)
                     /locus_tag="VFE39_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258279.1"
                     /translation="MVLLAVTVLTAGFAVLPLVATTVAGALAVVLGWGIAAWGFVPAQ
                     QHRLIGLGVGPARLVLALNSSATHLGFAAGALLGGLVVDAAGAGALWLLAVPCCGAGL
                     MLHAIPTPDRAGYA"
     gene            complement(604..1284)
                     /locus_tag="VFE39_06480"
     CDS             complement(604..1284)
                     /locus_tag="VFE39_06480"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HET6258280.1"
                     /translation="MADPSSSLAVGPTDQVPDLVMLPLADFRLSTENGRKVLRFTVHT
                     ENKGGTLDLTGSRSNASTASLTVVQNVHQTGGGVRSIRTNAAMRFAVADGHDHWHVSN
                     FAEYKLRPVGSSTWRGAHKEGFCARDTVHIEGNAPRSFPNNCEPGRSDALKVAEGIST
                     GWADRYDWDLWGQFIELDRLALPGDFCVAATADPLHLFAEAPKDNNTTSSLVRITAQD
                     VRVMRQGC"
     gene            complement(2418..2732)
                     /locus_tag="VFE39_06485"
     CDS             complement(2418..2732)
                     /locus_tag="VFE39_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006945020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET6258281.1"
                     /translation="MNATVGLLVTLEAKPGKEADVEEFLNAGRVLVDEERDTVAWFAI
                     KFGPKFGPATYGIFDVFSDEAGRTAHLQGEVAQALGARADELFDKQPEIQQLEVLASK
                     LP"
     gene            complement(3247..3786)
                     /locus_tag="VFE39_06490"
     CDS             complement(3247..3786)
                     /locus_tag="VFE39_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6258282.1"
                     /translation="MIHLDEQGRPHPPSASDETATLLGFLEFQRATLAWKCAGLDGAG
                     LGATLPPSSITLGGMLKHLAFVEDWWCSRWLHGRDPEPPWDTVDWDGDPDWDWHSAAD
                     DTPEQLHTLWRDAVARSRALVTEALADGGLEQLARRSNSGGEAPSLRWILFHLVEEYA
                     RHVGHADLLRESVDGLKGE"
     gene            complement(4050..4235)
                     /locus_tag="VFE39_06495"
     CDS             complement(4050..4235)
                     /locus_tag="VFE39_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258283.1"
                     /translation="MQRRDGGLQGESAGVATAERSQDQVVRLGDRLPDRTVVLFLPVL
                     EVVASTNSKPAGMVPPA"
     gene            4436..6046
                     /locus_tag="VFE39_06500"
     CDS             4436..6046
                     /locus_tag="VFE39_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258284.1"
                     /translation="MTPAAVERERVTMGVRKRCEVLRARIGRARALLRRAKELPYRME
                     ALDRHLAAIDAELHATRDDVVAVQSGLDRVLATVGESRQGVVELRDSVHGAARRTALR
                     RRPTRVLFLAHLVGVWDSYHALVETMAGSADFEPIVASIPRRFRGLDGLSGEDDVHRG
                     LTDRGVSHLRLTGDPGDVLRLIKAIEPDVIFRQSQWDADIPEELSTERLGFARTCLVP
                     YETMNIVVNVPNARTRNTAVDSDYHRVAWLVFCTNEMMLETAVRDGARGGAQFRLVGH
                     PKADHLRASRPDWPVCSTADDAPRPGRIVWSAHHTIAQGWTDFGAFHLMRDAMLGWAR
                     QCADVQFVFMPHPLLLQVPDSDASPVSRQDFDAWIKDWTDLPNTAVLSEANYGPALAA
                     SDIMVTDGLSMLVEYQLFDKPVIFFERDGHRPFNTIGEQVVTGVHTVHTVDEVRRLAE
                     AFVGGEPDPLRFRQRDNVRRLFGTGFSAERILAALRQEIARERGEADSVPAARRARRS
                     SGRQEAAKATRNRRPAGLDHAGEAPARG"
     gene            6237..6482
                     /locus_tag="VFE39_06505"
     CDS             6237..6482
                     /locus_tag="VFE39_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258285.1"
                     /translation="MLITVAGVPAHPLLVHAVVVLIPLAAFVVLAIGAALLGRRAIGS
                     ALRVVGSLSAVSGLVAITATVLAGHSGGQAVWGFVTS"
     gene            6619..7326
                     /locus_tag="VFE39_06510"
     CDS             6619..7326
                     /locus_tag="VFE39_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HET6258286.1"
                     /translation="MVRFYGEFLGPGDVGFDIGAHVGSRVRAWRKLGARVIAVEPQPD
                     CLRVLRLFFRRDREVTIVPDAVGARAGRARLGISTATPTVSSMSPDWIKSVTADRSFA
                     WVRWDRSVEVEVATLDDLIRVHGEPTFCKIDVEGFEVEVLAGLTRPLRALSFEYLPPA
                     HDAALAALDLVERLGAAAGGYEYNYSPIETMQFASDRWLDAADLVSLLERFRPLGRSG
                     DVYSRLTRPDREATSDH"
     gene            complement(7386..8003)
                     /locus_tag="VFE39_06515"
     CDS             complement(7386..8003)
                     /locus_tag="VFE39_06515"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6258287.1"
                     /translation="MLILVRHGQTDANARGLLLGRADPPLNEFGERQARALAAALPRP
                     ARILSSPLARARHTAAVLAGAAPGVTDTEDVEVDARWIEMDYGDLDGRPATALDERSW
                     RTWREDPDFVPAGGESLAAVCSRVHEACAELADDAARGDVVVVSHVSPIKAAVTWALG
                     VGHEVGWRMFLGDAAICRIDTSGRVPLLLAFNDPCPFDGSDRADH"
     gene            complement(7997..9070)
                     /locus_tag="VFE39_06520"
     CDS             complement(7997..9070)
                     /locus_tag="VFE39_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HET6258288.1"
                     /translation="MQRFWRYLRVLVGVGILAALVARLGTGAVVDGLRAIGTGSVLAA
                     LGIGLLTTVFSAWRWCLVARGLGLRLPMAEAVADCYRAVFLNSVLPAGVLGDVHRAVS
                     HGRQSGDVGRGVRAVVLERVVGQVVLLVVGVGVLLAQPTLLAATLGDLVPGGGVTVGM
                     LVVLAAVVVLGAWTMRGARASRIRNALRTGLADARTGVLSRGTWPGAVLLSVAALAGY
                     LALFVVAARAAGSQASLGELLPLLVLALFAMALPLNIGGWGPREAVTAVAFGAVGFDA
                     SQGLTAAVVYGVLSLIACLPGVGVLLLRGPISRWFHSAAGRPADVVGADVVGADVGAS
                     AEPIADIGVEWTSGPRRLVCREC"
     gene            complement(9070..9897)
                     /locus_tag="VFE39_06525"
     CDS             complement(9070..9897)
                     /locus_tag="VFE39_06525"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6258289.1"
                     /translation="MCAPDWLTLREGADASARAPELVERVRAHLAAGTRPGVPAVIRD
                     LGCGTGSMGRWLAGRLPGPQLWILRDRDPVLLARAGAGMPSVAADGTPVTARTEEGDL
                     TGLSAAELAGTSLVTASAVLDLLTAEEVDGLAAACVEAGCAALLTLTVVGSVEIAPSD
                     PLDVAFAAAFDSHQRRSTAGRRLLGPDAGAAAADAFALRGAAVETRPSPWRLGADQAE
                     LAEEWLRGWIGAACAQQPDLEQHAAAYLRHRLDACAAGELRVVVGHVDVLALPTGVS"
     gene            complement(9915..10997)
                     /locus_tag="VFE39_06530"
     CDS             complement(9915..10997)
                     /locus_tag="VFE39_06530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6258290.1"
                     /translation="MRSVHVVVPHDIDDVAVPSGGNAYDRRICRGLPATGWSVHEVAV
                     PGAWPRPDTAARSSLARTLSALPDDAVVLLDGLVACGVPDVVVPQASRLKLVVLVHLP
                     LGDEVGAAPADAAQLAALERETLHAASAIVATSMWSARRLVAHHGLAADRVHVATPGA
                     DLAPLAPGTDGATRLLCVGSITPTKGQDLLVEALAAVADLPWSCDLAGPLRRDPTHVD
                     VVRCAIERHGLGERVRLTGPRTRPQLATDYAAADLLVLPSRVESYGMVVTEALARGIP
                     VLATAVGGVPETLGHDPNGSAPGILVPPDDAPALAAALRRWFGGPELRGELRRAARDR
                     RSTLSGWEVTARCLARVLERLTGTRG"
     gene            complement(11021..11419)
                     /locus_tag="VFE39_06535"
     CDS             complement(11021..11419)
                     /locus_tag="VFE39_06535"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016331711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HET6258291.1"
                     /translation="MFTITVRDHVMIAHSFRGEVFGPAQRLHGATYVVDASFRRADLD
                     ADNIVVDIGLATQELGAVLADLNYRNLDDEPAFAGVNTSTEFLAKVIADRLAEKVHAG
                     ALGAGARDLAGITVVLHESHIASASYERSL"
     gene            complement(11441..12412)
                     /locus_tag="VFE39_06540"
     CDS             complement(11441..12412)
                     /locus_tag="VFE39_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HET6258292.1"
                     /translation="MARAFWLRAPGEGEIRSVAIPRPGPDDVEVRTLYSGVSRGTETL
                     VFRGGVPESQFSAMRAPFQDGEFPAPVKYGYLSVGVVEHGPPALLGRTVFCLYPHQTR
                     YVVPAAAVTPIPDGMPAARAVLAGTVETAVNVLWDAAPLVGDRIAVVGAGMVGCSVAA
                     LLARFPGVSVELVDIDPARAAVAEALGVGFASPEDAAGECDLVVHASATEAGLARSLE
                     LLAYEGEVIEMSWYGDRPVRVPLGESFHSRRLTVRSSQVGAVAPARRARRSFRERLTL
                     ALRLLADPVFDALVTDERRFEDLPRLMPDLTGGELPGLCVRVVYEQE"
     gene            12490..13269
                     /locus_tag="VFE39_06545"
     CDS             12490..13269
                     /locus_tag="VFE39_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET6258293.1"
                     /translation="MTTVARPQTSQERPRTAQQHVAGAAGQVVLLAVLSAGAGLGPVG
                     WLAGIAYLLGLWALLATAVRRAAATTLGPADLVTLARAGLVGGVTALVAEGIWSGHTA
                     VAPLVVIASTALLLDAVDGQVARRSGTVSALGARFDMEVDAFLVLVLSVHVALFVGPW
                     VLAIGVMRYAFVAASWSAPWMRSVLPTRYSAKAVAALQGIVLVVATSQFLLLPLAVAL
                     VSGALALLVWSFGRDVAWLWRTTHLRRPAATPRIPAPRSSR"
     gene            13266..15005
                     /locus_tag="VFE39_06550"
     CDS             13266..15005
                     /locus_tag="VFE39_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HET6258294.1"
                     /translation="MSSDDRISNGRIVGLSTTDDHPEHVAADEASAGESRRGRRHLVA
                     QVTTVLAGLLVFLALVAPREVGQLTPAALVRIPVEGLVGVVLLLVLPVRARTVVAVLG
                     GVVVGLLTILKILDMGFLAFLARPFDPVLDWVLVDDALSFVRGSFGQAGAIGSVVAAV
                     ALVAGVLVLMTFSVLRLSRLVVRHTATASRSVAVLSVAWLACAVLGAQIVPPVPIAAR
                     SAASLAYQEAVQVPASLHDQQAFAREAAVDAFRGTPGNQLLTALRGKDVIFSFVESYG
                     RDAVEDPEFASQVGAVLDDGNRRLSAAGFASKSAFLTSPVAGGGSWLAHATFFSGLWI
                     DNNQRDRNLVSTDRMTLTSAFQRANWQTVAVMPGTSEPWPERAFYGLDRLYDSHTIGY
                     HGPRFGWSPMPDQFALSAFERSEYGKPDRPPMMAEVVLTSSHMPWAPIPQFIDWGEVG
                     DGSVYGPMAAEGKSPDDVWSDPAQVPTEYRRSIEYSLTSLISWVENNGDDNLVLIFLG
                     DHQPLPIVTGDGASKDVPITIVARDKTVLDRISGWNWQDGLKPGPQAPVWPMNAFRDR
                     FLTAFGPQPERAR"
     gene            15215..15703
                     /locus_tag="VFE39_06555"
     CDS             15215..15703
                     /locus_tag="VFE39_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6258295.1"
                     /translation="MADASRRLVVVRHAKSAWPKGVPDAQRPLNERGRRDAPAAGRWL
                     RRHVEHIDAVRCSPAVRTRQTWELLSAMLDTAPDPVCDARLYGAAADMLLTIVRELPD
                     DAGCAMLIGHNPGMADIVTLLTGESPKMRTSAIAVLRLAMGWKKASPGGAALVAHATP
                     RG"
     gene            complement(15731..16159)
                     /locus_tag="VFE39_06560"
     CDS             complement(15731..16159)
                     /locus_tag="VFE39_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258296.1"
                     /translation="MVGASQVFVAPGPDDELLTATDLPWLAGRAVVLPAPQWQLVVTE
                     AALLDSRAAAELERVSGDFAYDSDPEFQLRDDELAALVRFLTDLGAHLAERRAFTAEP
                     PEDLEFLLDFEDYVGMVELVRLVIEESRRTGMPFRAWVEV"
     gene            complement(16205..24793)
                     /locus_tag="VFE39_06565"
     CDS             complement(16205..24793)
                     /locus_tag="VFE39_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258297.1"
                     /translation="MDDWRERYELEQGDGLRRTLAELGKPDPGRWWEETGPAARQMLS
                     LIRWHNAVTPGVVDGRRLTPRMVMASLRAIWADRRGDRADRRAAKQAARAEGGLGGAT
                     DTAALDLRDADGNAYTLEALVAAHQTSGAMRDQARAAWVLGEVQRTQLAQTRDAGGRK
                     ELRGTYTVKAIVAAGVMVGLGAAAVTVAPWALLPMSASAHYAAFAVRGALGLHRTAYS
                     QELRVLVESIMERGQFDIDTIRVLISVMRKYAALDGSTPTQLAGFDKVANDFLVIAHA
                     LVFSPLRPGQTAQGRSHAALKEFSLFLDKAAVELGAQAQSFQGFAPNAGLLGRVVNTV
                     LAGTYLVNLVVQAPAMVNGTGLDAWVNGAFALSSALFLVQALSVTVMGWAGIDFSSHP
                     VLRKVVHLGAVPAVTLGSLLLVIQTGVIDNSIMVVPALGLTAAGGYLSALGITIELGH
                     GRPAPRRGAGANAILSGSLLAFGAMSLLPVQWVGFSVGAVALLAGTWALSKLDTWRSR
                     RTRGPPASGAPENPTSGSSGPSGGPSAGGGTGISGPPRSGGPRSPASGSGNALQPGGS
                     GTAVPAQGARNSRPEAASRLTGAADLRAKRLRAALRLRAALRMLGRAPVVVGLLTTGA
                     VVSDWFGAAVAQAGTRGGTVDTGGSVAAGWWGGATGLTAAGVAVAVGAVVVGVRGWWT
                     PRQARAPPRAVLAAWGRGLRAWWSGVVSGLEFGLGVRISARPLLVSRAWWGAVARLQV
                     AAVAGGVRSARTRVGAASTPAAYLATRALRAAVRGMGDQLSGAVTDLIETRRVAELAR
                     GMYAQRFGWPARLVEELTKDPGFAGRDIVQLAADALGIAREEVHERVLTDRWQAAPDI
                     EYFNLTGRKYLDAIATETATRREGLRKADELHSAARDAVRDLVGAAIARAVAGGAPER
                     RIARALEVPPALIGVLTGRATRGPPAARGSWGRAPRWPGRVRRAAVGSGRTGDGPGAA
                     RLEHMQASLGFSERRAWVGRAFDQLHGRRDDDWIHEQLASALRLDEVEVRSIVASSVA
                     SPRAPPGVVGGRLSPDALIGKLVELTEQRRVRLQKANELVARIEPEADGRDVAAGPPI
                     VTDWLILPALSQMRSLGLKVTGRGDRVRLAFPEGGVDPRLSEAVVALRKFADGHLHHS
                     GYPYHRATSLGGLVAKMLDAGYDVVLTFHPIPQRIKGLAYYGDVDAQVATTRFGVGML
                     AMRGKLTDAHVLAAIRALPAHLAWRVVAGITGGRFDIPGLKIYLMVTTLVNAGLAFLL
                     GEKTFNKENVKIQLGPQAANLLDAGYVRTLERLRAVVPLLRAAFADGRLDRADFPRLS
                     AVVDDLYSAAAGKVALRIVRKLHELADPTVADPAALWELRDALTVLGVLREGEGLGRY
                     RIGRVYGQGLVDYLEFLTASGAIGVVHNDAGEPLLGEDGFPIAGKPDWRYLIAFLAAL
                     RDFPEARVILAHIGTGKFTTLSREYLDFLEKEVFEVRTEKYPNLHADLSWGDLAQHIE
                     ETPALRQHFVRLGSRFHRFFILGTDGVKPATVLHYLRHHRDMANILADVDRLAPGAYE
                     RIGYRNLIALLTVARRDVRAWTLANVGSGVWDGVLRAFPGMVRQRVRDWVALQRLYGV
                     GTTQTARTPGSSPRPAPVHDPGPRPFDDQQLADMLGWYRAVDERSASGRQTLRLWRHG
                     VRVTAREWRRGHGSGLQQPADARAAVEAAVAPQDLPGGSYTLEALIVADQAQRVGTSG
                     VHQRVALELVQERQEQVNHAREVIAAKAGLVRRAFRAWTAGVTAVAAGAVLAGLWLIP
                     AALPWISSIAFVVRGAATLLKTAEQSAVREVHDSMMERGRITRAKITWLYRMVVKNAR
                     AYGVDEQRLPEFTRVTREFLTNMYDRDKGLLRKPLQDGETLRDRLGRVIADYSRWSDG
                     STRAIGPSITSLQSTGAQARWGRAISAAVAVTWVGNLAHHMHATYVGLKVGDQWLETV
                     TNGFYVGADLLFMAIALPTAVSGWFRFNIASWPWARRLNNYLAFPVVTLANVGLTALF
                     MTQNLPAAAVVSGAAAATGAVLSVKVVASAVLTGSTGYLTTLGLQTELVLGVRAARRL
                     PTATTTFALSLIGLGLMTAPRPLLADVPLLVPVVAVAALAVARTTVTTAYRYVTTGQG
                     VRAALLTRQGALSLSTTIAAVGLGVHQAVPGLGGPALVAAVAAGVAVVGAATTATVTL
                     VEKARVAATDANLRPIRDRDAAIAGIRAGRSVRWGARLARVDREQVIRWLVAALRTRG
                     PPRTMLTGSAYTELVSGARQYPLRRPWRLAGHLAAALRAGVAPAQLGTTAGRHSIREY
                     PLLIWVLRTDGSIWVAPARVRGPGRRPARISHPMLSEGRDVLAAGQVVLDIDGARLVG
                     RSIDVRTRHYRGHGTATPGQGLHTIALAAFHHHGIRFPDPARPSGGSTDDSEQPGGDA
                     GRSTVGALFTFGAVAISLAALYVLAGPAPVVVAIGAGVATAVTARIGSVQGAARRAAG
                     RVTTWTHAWSQRGPPAVVTDDPIAGQRYSRPEQGGPAAATGPTGTRTAQRLLALARSW
                     VANRATALRNAGTWPAPNAVVRDLLDLVPGNPSRGFKVTRSSARSDDDLLTSVFPPID
                     GQFLATHPSTPASIPPGAGPARVGADDADATRPLTAQQQEIIDREVRKHGHQLVKTPQ
                     TRAKFTRDPQQYAREVSWVAAVVGDVLEALAAPVMQRLAAERGEQVLKNVVARPVTAS
                     GGSAGDLVEFDFLFFDAAGPVRYVSAKANPKKFTPAEDRVKLARLFSEIPTESPEALR
                     AYLAYLRQNPATLAKIVDVGVSWDGSDGAIPLAEFRRLLVRGADAGDIAIEMMTPAGP
                     RARGHQIDLTRDELVDAVIAGIRRRVAAIEAEDR"
BASE COUNT         3825 a         9067 c         8476 g         3465 t
ORIGIN      
        1 gcgggtcgcg gtcacgcata tcccgcgcga tccggcgtcg ggatcgcgtg caacatcagc
       61 ccggcaccac agcaagggac cgcgagcagc cacagcgcgc cggccccggc ggcatcgacg
      121 acgagaccac cgagcagcgc accggccgcg aacccgaggt gggtcgccga actgttcagc
      181 gccagcacga gccgcgccgg ccccacgccg agtccgatca gccggtgctg ctgtgcggga
      241 acgaatcccc aggccgcgat cccccacccg agcacgaccg ccagcgcacc ggccaccgtc
      301 gtcgcgacga gcgggagaac cgcgaaaccc gccgtcagca cggtcaccgc cagcaggacc
      361 acacggcggc tgccaccccg gtcggccgca gtcccgcccc accagaaacg agcagatcat
      421 gacccgccac aatggccggg cctcgagcag agcgctcagt agcggcccaa ctggcgctgt
      481 cgccaccctg acacggccat tgcggctcgc ggcagcgccc cggaagccgc gcatggcccg
      541 ccgacgggat gtgtcgtcct ggccgtcgag gcacaggcac acaccgtcca ggtggctgcc
      601 cagtcagcaa ccctgccgca tcactcgcac atcttgtgcg gtgattcgga ccagggacga
      661 cgtcgtgttg ttgtccttcg gcgcctcggc gaacaagtgg agtgggtccg cggttgcagc
      721 cacgcagaaa tcacctggaa gagccagacg atcgagctcg atgaactggc cccacaagtc
      781 ccagtcgtaa cgatctgccc aaccggtgga aattccctcg gccaccttca gtgcgtctga
      841 tcggccgggt tcgcaattat tggggaacga cctgggagcg ttcccctcga tgtggacggt
      901 atctctggcg cagaaaccct ccttgtgagc ccctcgccaa gtactcgagc ccacgggacg
      961 aagcttgtac tccgcgaaat tggacacgtg ccagtgatca tgtccatcgg caacggcgaa
     1021 gcgcattgcc gcgttggttc gaatactgcg aactcctccg cctgtttggt gcacgttctg
     1081 gacaaccgtc aggctcgccg tgctcgcgtt ggatcgggaa ccggtaagat caagagtacc
     1141 gcccttgttc tcggtgtgca ccgtgaaccg caggaccttg cgtccattct ctgtactgag
     1201 gcgaaagtcc gcgagaggca gcatgaccag gtctggtacc tggtcggtcg ggccaacggc
     1261 cagggaggac gacgggtcag ccacggcccc tcttatgcca taggcaagaa tgagtacgga
     1321 ggcaaacgcg acactaaagg ccgcgaccaa ccaccgtgga tagttcagag tctgggacat
     1381 cccgaccccc tgtctattcc ggctacgctg gcgggaacgc gcattccatc gcggtgttgg
     1441 cgagcagttc aacatggacg gttcagccag ccacctacct gcacgatcac gcatgagtca
     1501 actgggtcgg taggtacctc gcagtgtcgt gatcggacgt tacgtggaag atcatcagca
     1561 acaagattct cgatcacaac cagcagggtc gcgaaccctg gcgtgagccc atcgcagata
     1621 ctcggagatc attgttcgat gatctctgag taggagttgg aagcagttga tgtgagctcg
     1681 tgaacccggc ccgccaatct tcgccgtgta catcatcttt tggccagtca atctggcgcg
     1741 gtcagtgggc ttacctgcga cgcgttccga tgcctcgtga tttgtggccg tgctgctcct
     1801 tgagagttac cgcacgcctc gacgaagcgc aggttggacc tgtgcaggcg aattccgcgt
     1861 gaattcaact cggccgaagt agatccatgc caccccgtca tcgccggccc gcggccacgt
     1921 cggattgggt cgccggcacg ccaccgccga gccaggtcga cacgcacgcg tgccgagcac
     1981 gtacgtggtg gcggcgagcg acgagggcgc cgcttctgcg cggtgaacgc tcgccggccc
     2041 gcgacgcgtc acgtgctcgg gtgtgtcggt ttgggcagtt cgtccgcccg gtcccccaga
     2101 aacagcagcc gccggcgccc gtccgaactc cgcggggtac tcgtgtagga aagccgtccg
     2161 cggggcgtag gccggcgaac tacatgaggg cgaagagggc gacggaccgg cggccactgc
     2221 ggtgcgtatc cctgacggtg ttcagcagcg ggcaatcttt ggtcgagctg cgggctggag
     2281 acagcgacac gagtgcctac gccggcgcgc cgatgccgga gatcgggatc ctgccctagg
     2341 ctcaactcgc tgttggagga ccagagcgac gtcgaatggc taaaagaagt aggttctgcg
     2401 ttccaacagg attccggtca gggtaacttc gaggccaaca cctcgagctg ctggatctcg
     2461 ggttgcttgt cgaacagctc gtcggcgcga gcaccgagag cctgagcgac ctcgccctgc
     2521 aggtgcgcgg tccgtccggc ctcgtcggag aacacgtcga agatgccgta cgtggccggc
     2581 ccgaacttgg gcccgaactt gatcgcgaac caggcgacgg tgtcccgctc ttcatcgacc
     2641 agcacgcgac ccgcgttgag aaattcctcg acgtcagcct ccttgccggg tttggcctcc
     2701 agggtcacca gcagtcccac cgtcgcgttc atttcctact cacctctcgg tcgtggtgat
     2761 cgtgcggtcc aatgacgagc cttgggccgc ccagacgaca acacatccat cagccgggtc
     2821 agaatcagca cgacccggtg gacctgcaag gccgacacgg ttacctggcg gccacgatgc
     2881 ccgttcatcg ggccgccacg cgtcgctgcg gtagtgctct ctcctcgccc tcagcgcgat
     2941 gctcgcccgg tgggcgcggc ggttgatccc caagtacgtc gctcccgcac cctcaccagg
     3001 acgacctcac ctgatccggc tcgaccctgc tgtcccccga cgacggtcag gcccgcccgc
     3061 cggagctggt ctcgaccacg acggccggga cgggcgtcct catctgcacc taccggccgg
     3121 ggcgccgctg acgctgccac acacgagatc atcgagaggg accggcgagg gcccgctccg
     3181 gccatcagcc cggaccggcc ctcccggcat cgatgggatc gtggggatac cgcagccgcc
     3241 cggccgctac tcccctttca gcccgtccac cgactcccgg aggaggtcgg catggccgac
     3301 gtgccgggcg tactcctcga ccaggtggaa caggatccat cgcaggctgg gtgcctcacc
     3361 gccggagttg ctgcgccggg ccagctgctc gaggccgccg tcggccagcg cctccgtgac
     3421 cagggcgcgg gagcgggcca cggcgtcccg ccagagcgta tggagctgct cgggggtgtc
     3481 gtcggcggcc gagtgccagt cccagtcagg gtcgccgtcc cagtccaccg tgtcccacgg
     3541 gggctccggg tctcgcccgt gcagccaccg ggagcaccac cagtcttcga cgaaggccag
     3601 gtgcttgagc atcccgccca gggttatcga cgaggggggc agcgtcgccc ccaggccggc
     3661 cccgtccagt cccgcgcact tccacgccag cgtcgcccgc tggaactcaa ggaagcccag
     3721 gagggtggcg gtctcgtcgg acgcgctggg agggtgcggg cgaccctgct catcgaggtg
     3781 gatcatggtc ggtgaaccta ccggcgcggc caacagccga tggtccgcca cgtattcggt
     3841 cctcggccgc cccacccgcg ccggaatcgg ccgttgctcg aagccctgcg tcaggtgttg
     3901 tagccgctcg cgtggtcacc gggtcaggcg ttcgggctgg tccgagcgat gtcggtgggt
     3961 tcgagacgaa ccaggcgtcg ggcgcgagac cgtgtcaacg ggctccacct gctggtcttc
     4021 ccggtgctca tcggctccgg caagcgtctc tacgccggag gaaccatccc ggccggcttc
     4081 gagttggtcg acgccacgac ctcgagcacc ggcaggaaca ggaccaccgt tcggtctggg
     4141 aggcgatcgc cgaggcgcac gacctggtcc tgggagcgct ccgcggtggc aaccccggcc
     4201 gattcgcctt gcaggccgcc atcgcgacgc tgcatgcctc cgatcggacg tgtccggcag
     4261 cgcccggcgg caggaccgtg gtcccgcatg atgcccgaat tgcacccgcc gtaacctggg
     4321 gctgtgttgc acgcgctcgt cacctgaaag tcgggcggcg ggcatttgtc gttcactgtc
     4381 cagacgccag cgatggcgcg tcgcctcgcc gaacgtcagg gcaggacgac cccgcatgac
     4441 gccggcagcg gtcgaacgcg aaagggtgac gatgggcgta cggaagcggt gcgaggtcct
     4501 acgcgccagg atcggtaggg cgcgagccct gctgcggcgg gccaaggagc tgccctaccg
     4561 gatggaggcg ctcgaccggc acctcgcggc catcgacgcc gagctgcacg ccacccgcga
     4621 cgatgtcgtc gcggtgcagt ccgggctcga ccgggtcctc gcgacggtcg gtgagtcccg
     4681 gcagggggtc gtcgagctgc gagactccgt ccatggagcc gcccgccgca ccgccttgcg
     4741 gcgccggccg acccgcgtgc tgttcctggc gcacctcgtc ggtgtgtggg actcctacca
     4801 tgcgctcgtc gagacgatgg ccggcagcgc cgacttcgag ccgatcgtcg cgtcgatccc
     4861 ccgccgattc cgcggcttgg acggcttgag cggcgaggat gacgtgcacc gcggcctcac
     4921 cgaccggggt gtatctcatc tacgcctcac cggcgaccct ggtgacgtcc tccgactgat
     4981 caaagcgatc gaacccgacg tgatcttccg gcagtcccag tgggacgccg acatcccgga
     5041 ggagctgagt accgagcggc tgggcttcgc ccgcacctgc ctggtcccct acgagacgat
     5101 gaacatcgtc gtcaacgtcc ccaatgcacg gacgcgcaac accgccgtcg acagcgacta
     5161 ccaccgagtc gcatggttgg tcttctgcac gaacgagatg atgctcgaaa cggcggtccg
     5221 ggacggcgcg cgcggtggag ctcagttccg actggtaggc catcccaaag ccgaccacct
     5281 gcgggcgtcg aggccggact ggccagtgtg ctcgacggcg gacgatgcgc cccgacccgg
     5341 ccgcatcgtc tggtccgccc accacacgat cgcgcagggt tggacggatt tcggggcctt
     5401 ccacctcatg cgcgacgcca tgctcgggtg ggccaggcaa tgcgccgacg tccagttcgt
     5461 gttcatgccg cacccgctgc tgctgcaggt ccccgactcg gacgcctctc cggtcagccg
     5521 ccaggacttc gacgcctgga taaaggactg gaccgatctg cccaacaccg cggtgctgtc
     5581 cgaggcgaac tacggcccgg cgctcgccgc ctcggacatc atggtgacgg acggactgtc
     5641 gatgctggtg gagtaccagc tcttcgacaa gccggtgata ttcttcgagc gcgatggcca
     5701 tcggccgttc aacaccatcg gcgaacaggt cgtcaccggc gtccacaccg tgcacacggt
     5761 cgacgaggtg cgccggctgg ccgaggcgtt cgtcggcggc gaaccggacc ccctgcgatt
     5821 ccggcagcgg gacaacgtgc gcaggctgtt cggcaccggc ttcagcgctg agcgaatcct
     5881 tgcagcactg cggcaggaga tcgcgcgcga gcgaggcgag gcggactccg tcccagcggc
     5941 ccgacgcgca cggcggtcct ccggtcgaca agaagctgcc aaggcgacgc ggaaccggag
     6001 gcccgccggg ctcgaccacg cgggcgaggc gccggcgagg ggatgaccgc ccgttcggct
     6061 gaccgggacg ctcgtgatca tcggggccgg cggacggtgg ctcggagggc gtgtgaggcg
     6121 cacaccgatc ggatgcggtc tcggaagccc gatatcgcga cggcatcgtc ccgaggtgtc
     6181 acgctcgtct ctgctcgcct ggtcgtggtc gggcgccagt cgtcgagaat tctggggtgc
     6241 tcatcacggt cgcaggcgtt ccggcgcacc cgctcctcgt ccacgcggtg gtcgtcctga
     6301 tcccgctggc cgcgttcgtg gtgctcgcga tcggcgcagc cctgctcggt cgccgtgcga
     6361 tcggttcggc gctgcgggtt gtcgggtccc tgtccgcggt ctcgggcctc gtggcgatca
     6421 ccgccactgt tcttgccggg cactcgggcg ggcaagcggt ctggggcttc gtcacctcct
     6481 gaccctcccc tgtggatgga caagacttgt cgaatcttca ggaccggttc cgcggtaggg
     6541 ttcggcatgc tccgccgcgc ccgggggatc gcgcgatccc tggccgtgta ccacggtgtg
     6601 ccgggcaggc accgtcgtat ggtgcggttc tacggcgagt tcctcgggcc gggcgatgtc
     6661 ggcttcgaca tcggcgccca cgtggggagc cgggtgcgcg cctggcggaa gctgggggca
     6721 cgggtgatcg cggtcgagcc gcagcccgac tgcctgcgcg tcctgcgtct cttcttccgt
     6781 cgtgaccgag aggtgacgat cgtgccggat gctgtcgggg ccagggcagg gcgggcgcgg
     6841 ctgggcattt ccaccgcgac gccgaccgtc tcgtcgatgt cgcccgactg gatcaagtcg
     6901 gtcactgcgg accgcagctt cgcctgggtg cggtgggacc gctcggtcga ggtggaggtg
     6961 gcaaccctcg acgacctcat cagggtccac ggcgagccga ccttctgcaa gatcgacgtc
     7021 gaaggcttcg aggtcgaggt gctggccggg ctcacccgac ccctgcgcgc gctgtccttc
     7081 gagtatctgc cgccggcgca cgacgccgcc ctcgccgcgc tggacctcgt ggaacggctc
     7141 ggtgcagcgg cgggcgggta cgagtacaac tactcgccca tcgagacgat gcaattcgcc
     7201 agtgaccgct ggctcgatgc cgccgatctg gtgtcgctgc tcgagcgatt ccgcccgctg
     7261 ggccgctccg gcgacgtcta ctcccgcctg accaggccgg accgcgaagc gacgtcggac
     7321 cactaggccg aacgcggagc ggtccacgcg gacaggctcg cgcatgctgt cgacccgcta
     7381 gcaggtcaat ggtccgcccg gtcgcttccg tcgaaggggc acgggtcgtt gaaggcgagc
     7441 agcagcggca cccggccgct ggtgtcgatc cgacagattg ccgcgtcccc gaggaacatc
     7501 cgccagccca cctcgtgacc gacccccaac gcccaggtga ccgccgcctt gatcggggag
     7561 acgtggctga ccaccaccac gtcaccgcgc gcggcgtcgt cggccagctc ggcgcaggcc
     7621 tcatgcaccc gtgaacagac cgccgcgaga gactcgccgc cggccggcac gaagtccggg
     7681 tcctcccgcc acgtccgcca cgaccgctcg tccagtgccg tcgcaggcct gccgtccagg
     7741 tcgccgtagt ccatctcgat ccatcgcgcg tccacctcca cgtcctcggt gtctgtgact
     7801 ccaggggccg cgccggcgag tacggcggcg gtgtgacgtg cgcgcgccag cgggctggac
     7861 agaatgcgag ccggacgcgg aagtgccgcg gccagagccc gcgcctgacg ctccccgaac
     7921 tcgttcagcg gcgggtccgc ccggccgaga aggaggccgc gagcgttggc gtccgtctgt
     7981 ccatgccgga cgaggatcag cactcgcgac acactagtcg tcgagggccg gacgtccatt
     8041 cgacaccgat gtccgctatg ggttccgcag aggccccgac atcagcgccc acgacatcag
     8101 cgcccacgac atcagccggc cgtcctgccg ccgaatggaa ccacctgctg atcgggcccc
     8161 gcagcagcag caccccgacg ccgggaaggc aggcgatcag gctgagcacc ccgtacacca
     8221 cggcggcggt gagcccctgc gacgcatcga accccaccgc cccgaacgcg accgcggtga
     8281 cagcctcgcg ggggccccag ccgccgatgt tgagcggcag tgccatggcg aacagcgcca
     8341 gcaccagcag cgggagcagt tcgccgagcg aggcctggga tcccgcggcc cgtgcggcga
     8401 cgacgaagag cgcgaggtac ccggccagcg cggctacgga gagcaggacg gcgcccggcc
     8461 aggtgccacg ggagagcact cccgtgcggg cgtccgcgag gcccgtccgc agagcgttgc
     8521 ggatcctcga cgcgcgtgcc ccgcgcatcg tccatgcccc gagcacgacg acggcggcga
     8581 ggacgacaag catcccgaca gtgacaccgc cgccggggac caggtcgccg agagtggccg
     8641 cgagcagggt cggttgggcg agcagcacac ccaccccgac gaccagcaga accacctgac
     8701 cgacaacgcg ttcgagcacc accgcccgga cgccccgtcc gacgtcgccg gactggcgac
     8761 cgtggctcac cgcccggtgc acgtcgccga gcacacccgc cggcagcacg gagttgagga
     8821 acacggcccg gtagcagtcg gcgaccgcct ccgccatcgg caaccgcagg ccgagaccgc
     8881 gcgccacgag acaccaacgc cacgcactga acaccgtggt cagcaggccg atgccgaggg
     8941 cggcgagcac cgatccggtc ccgatcgccc gcagcccgtc gacgacggcg ccggtaccga
     9001 gccgcgccac gagggccgcc aggatgccga cgccgacgag cacccgcaga tagcgccaga
     9061 acctctgcat cacgacacac ccgtcgggag cgcgagcacg tcgacgtggc cgaccacgac
     9121 ccgcagctca ccggccgcgc aggcgtcgag cctgtggcgc aggtaggcgg ccgcatgctg
     9181 ctcgaggtca ggctgctgcg cgcacgcggc gccgatccag ccccgcagcc actcctccgc
     9241 gagctcggcc tggtccgccc cgagccgcca cgggctgggc cgggtctcca ccgccgcacc
     9301 ccgcagggcg aacgcgtcgg cggcggcggc acccgcgtcc gggccgagca gtcgcctgcc
     9361 ggccgtactt cgccgttgat gggagtcgaa ggcggccgcg aaggcgacat ccaacggatc
     9421 ggagggagcg atctcgacgc tcccgacgac cgtgagcgtc agcagcgcgg cgcatccggc
     9481 ctcgacgcac gcggcggcga gtccgtccac ctcctcggcg gtgagcaggt ccagcacggc
     9541 ggacgcggtg accagcgacg tcccggcgag ctccgctgcg gacagtcctg tgaggtcccc
     9601 ctcctccgtc cgcgcggtga ccggagtgcc gtcggcggcc acactcggca tgcccgcgcc
     9661 cgcacgggcg agtagcacgg ggtcgcggtc gcgcaggatc cagagctgcg ggccgggtag
     9721 ccgtccggcg agccagcgcc ccatcgagcc ggtgccgcag ccgaggtccc tgatcaccgc
     9781 cgggacaccg ggccgggtac cggccgccag gtgggcgcgg acccgctcga cgagctcggg
     9841 tgcccgggcc gaggcgtcag cgccctcccg cagcgtcagc cagtccggcg cacacaccgg
     9901 cgatccgggc acgctcagcc tcgcgtccca gtcagccgct ccagtactcg ggccaggcat
     9961 cgtgccgtca cctcccaccc ggaaagcgtg ctgcgccgat ccctcgctgc gcggcgtagt
    10021 tcgccgcgca gctcgggccc gccgaaccag cgacggaggg ctgcggcgag tgccggagcg
    10081 tcgtcagggg gtaccaggat ccccggcgct gaaccgttcg ggtcgtggcc gagtgtctcc
    10141 ggcaccccgc cgacggccgt cgcgagcacc ggaatccccc gggccagcgc ctcggtcacg
    10201 accatcccgt aggactcgac gcgggacgga agcaccagaa ggtcggccgc ggcgtagtcc
    10261 gtggcgagct gcgggcgcgt ccgcggaccg gtgagccgta cccgctcgcc gagcccgtgg
    10321 cgctcgatcg cacaccggac cacatccacg tgtgtcggat cgcggcgcag cggcccggcg
    10381 agatcgcagc tccacggcag gtcggcgacg gcggcaagag cctcgacgag caggtcctga
    10441 cccttggtcg gggtgatcga cccgacgcac agcaggcgcg tcgcaccgtc cgtgccgggg
    10501 gcgagcggcg cgagatcggc acccggcgtg gcgacgtgta cccggtcggc ggcgagcccg
    10561 tgatgcgcga cgagccggcg cgccgaccac atgctggtgg ccacgatggc gctcgcggcg
    10621 tgcagggtct cccgctcgag ggcggccagc tgcgccgcgt cggcgggcgc cgcccccacc
    10681 tcgtcgccga ggggcaggtg gaccaggacg acgagcttca gccggctcgc ctgcgggacg
    10741 acgacatccg gtacgccgca ggcgacgagc ccgtcgagga gcacgacggc gtcgtcgggc
    10801 aaggcggaga gggtgcgggc caacgacgaa cgggcggcgg tgtcgggtcg gggccacgct
    10861 ccggggacgg cgacctcgtg caccgaccag ccggtggccg gcaggccgcg gcagatgcgg
    10921 cggtcgtacg cgttgccgcc gctcggaacg gcaacgtcgt cgatgtcgtg cggcacgacg
    10981 acgtgcacgg agcgcaggtc tgcagcgaga ccgctcgccc tcacaaggag cgctcgtagc
    11041 tcgccgaggc gatgtgcgac tcgtgcagca cgaccgtgat cccggccagg tcgcgggccc
    11101 cggcacccag cgcgcctgcg tgcaccttct cggcgagccg atcggcgatg accttggcga
    11161 ggaactcggt ggacgtgttg acgccggcga aggcgggctc gtcatcgagg ttgcggtagt
    11221 tgaggtcggc caggacggca ccgagctcct gggtcgccag cccgatgtcg acgacgatgt
    11281 tgtcggcgtc gagatcggca cggcggaacg acgcgtccac cacataggtc gccccgtgca
    11341 gccgctgcgc cgggccgaag acctctcccc ggaaactgtg agcgatcatg acgtgatcac
    11401 gaacggtgat ggtgaacaag acctgccctt ccagcgatgc ctactcctgc tcatagacga
    11461 cacggacgca cagcccggga agttcaccac cggtgagatc gggcatcagc cgaggcaggt
    11521 cctcgaagcg tctctcgtcc gtgaccagcg cgtcgaacac cggatcggcg agcaggcgca
    11581 gtgccagagt cagccgttcc cggaagctgc gacgggcacg ccgagccggt gcaacggccc
    11641 ccacctggct gctgcgcacc gtcaggcggc gcgagtggaa cgactcaccg agcggcaccc
    11701 gcaccggccg gtcgccgtac cagctcatct cgatgacctc gccctcgtag gcgagcagct
    11761 cgagggatcg cgcgagcccc gcttccgtgg cgctggcgtg caccaccaga tcgcactccc
    11821 ccgcagcatc ctccggcgag gcgaacccga cgccgagcgc ctcggcaacg gctgcacgcg
    11881 cggggtcgat gtccaccagc tcgacgctga ccccggggaa gcgggccagc agtgcagcga
    11941 cgctgcagcc gaccatcccg gctccgacga cggcgatccg gtcgccgacc agcggggccg
    12001 catcccacag cacgttcacc gcggtctcga cggttccagc gagcaccgcg cgggcagccg
    12061 gcataccgtc ggggatcggc gtcaccgccg ccgccggcac cacgtagcgg gtctgatggg
    12121 gatagaggca gaacacggta cgaccgagca gcgcaggggg gccatgctcg accactccca
    12181 cgctcagata gccgtacttg accggcgcgg ggaactcgcc gtcctggaag ggcgcccgca
    12241 tcgccgagaa ctggctttcc ggcacgcccc cgcggaagac gagcgtctcc gtgccgcggc
    12301 tgacccccga gtacagggtg cggacctcca cgtcgtcggg gccgggccgg ggtatggcga
    12361 ccgaccggat ctcaccctcg cccggagcgc ggagccagaa ggcgcgcgcg atgcgatcca
    12421 ctcatccctc cacatcgtga tgaacccatg ccgcgtcgga tccgtgctga gtatgtgcat
    12481 agaggctcga tgacgaccgt agcccgccca cagacctcgc aggagcggcc ccgaaccgcg
    12541 cagcagcacg tcgccggggc agctggccag gtcgtcctgc tcgccgtgtt gtcggccggt
    12601 gccggcctcg gcccggtggg ctggttagcc ggcatcgcgt acctcctggg cctctgggcc
    12661 ctgctggcca ccgccgtccg ccgggcggcc gccaccacac tcggcccggc cgacctcgtc
    12721 acgctggcga gggcgggact cgtcgggggc gtcaccgcgc tcgtcgcgga ggggatctgg
    12781 agcggtcaca ctgcggttgc acccctcgtc gtcatcgcct ccacggcgct gcttctcgac
    12841 gcggtcgacg gccaggtcgc ccggcgcagc ggcacggtgt ccgcgctggg cgcccgcttc
    12901 gacatggagg tcgacgcgtt cctcgtcctc gtgctgagcg tccacgtcgc actgttcgtg
    12961 ggcccgtggg tgctggccat cggcgtcatg cgctatgcgt tcgtcgccgc gagctggtcg
    13021 gcgccgtgga tgcggtcggt actgccgacc aggtactccg ccaaggctgt cgccgcgcta
    13081 caggggatcg tcctcgtggt ggcgaccagc cagttcctgc tgctcccgct cgcggtcgcg
    13141 ctcgtgtcgg gcgcgcttgc cctgctggtc tggtccttcg gccgtgacgt ggcgtggcta
    13201 tggcgcacaa ctcacctgcg gcgaccggcc gcgacaccgc ggatccccgc accaagatcg
    13261 tcgcggtgag cagcgacgac cggatctcga acggaaggat cgtcgggttg tcgacgacgg
    13321 acgaccaccc ggagcacgtg gccgccgacg aggcatcagc tggcgaaagc cgccgaggaa
    13381 ggcgtcacct cgtggcgcag gtgacgaccg tcctggccgg actgctcgtg ttcctcgccc
    13441 tcgtcgcccc gcgggaggtc ggtcagctca cccctgctgc gctcgtgcgc atcccggtgg
    13501 aaggactcgt cggcgtcgtc ctcctcctcg tcctgccggt gcgggcgagg acggtggtgg
    13561 cggtgctcgg cggggtggtc gtcggcctgc tgaccatcct gaagatcctc gacatgggct
    13621 tcctcgcgtt cctcgcccgg ccgttcgacc cggtgctcga ctgggttctg gtcgacgacg
    13681 ccctgtcctt cgtccggggc tcgttcggcc aggcaggcgc gatcggctcc gtggtcgcgg
    13741 ccgtggcgct cgtcgccggc gttctcgtcc tcatgacgtt ttcggtcctg cgcctgtccc
    13801 gcctggtggt ccggcacaca gccacggcga gccggtccgt cgcggtgctc tcagtggctt
    13861 ggctcgcctg cgccgtgctc ggcgcgcaga tcgtcccgcc cgtgccgatc gccgccagga
    13921 gcgcggcctc actcgcctac caggaagcgg tccaggtgcc cgcgagcctg cacgaccagc
    13981 aggcgttcgc cagagaggcc gcggtcgacg cgttccgcgg caccccgggg aatcagctgc
    14041 tgaccgcgtt gcgcggcaag gacgtcatct tctccttcgt cgagagctac gggcgcgacg
    14101 cggtcgagga tccggagttc gcgtcgcagg tcggagcggt gctggacgac gggaaccgcc
    14161 ggctcagcgc agccgggttc gcgtccaaga gcgcattcct cacctcaccg gtggcgggcg
    14221 gcggcagctg gctcgcccac gccaccttct tctccggcct gtggatcgac aacaatcagc
    14281 gcgaccgcaa cctcgtctcg accgaccgca tgaccctcac cagcgccttc cagcgcgcga
    14341 actggcagac ggtcgccgtg atgccgggca cgagcgagcc ctggccggag cgagcgttct
    14401 acggcttgga ccggctctac gacagccaca ccatcggcta ccacggcccg aggttcggct
    14461 ggtcgcccat gcccgaccag ttcgcgctgt cggcattcga gcgctccgag tacgggaagc
    14521 cggaccgccc cccgatgatg gcggaggtcg tcctcacctc gagccacatg ccctgggcgc
    14581 ccattcctca gttcatcgat tggggcgagg tcggtgacgg ctcggtctac ggccccatgg
    14641 cggcggaggg caaatcgccc gacgacgtgt ggagcgaccc cgcacaggtg cccaccgagt
    14701 accggcggtc catcgaatac tcgctgacca gcctcatctc atgggtggag aacaacggag
    14761 acgacaacct cgtgctcatc ttcctcggcg accaccagcc actgccgatc gtcacgggcg
    14821 acggcgccag caaggacgta ccgatcacca tcgtcgcccg cgacaagacc gttctggacc
    14881 ggatctccgg gtggaactgg caggatggcc tcaagcccgg cccgcaggca ccggtctggc
    14941 cgatgaacgc cttccgcgac cggttcctga ccgccttcgg accgcagccc gagcgcgctc
    15001 gatagcaccc cgacggcggc cggctccccg agtcacgccg gcagcaaccc gaccccacca
    15061 gccgggggat ccggctcggg agcgctgttg ctggtcgggg cacggggcga tgcggaccgc
    15121 taccgtgtcc cgatgggcag cagctgctct caccggtgtc ctgtttcgga ccccgcccga
    15181 tgcccgcggt cgggcagccg ggactggacg cgacatggct gacgcttccc gccggttggt
    15241 ggtcgtccgg catgcgaagt cggcgtggcc gaagggcgtg cccgacgcgc agcggcccct
    15301 gaatgagcgc ggccgacgcg atgcgcccgc ggcaggccgc tggctgcggc gccacgtcga
    15361 gcacatcgac gctgtgcggt gctccccggc ggtgcggacg cgccagacct gggagctgct
    15421 gtccgccatg ctcgacactg cgcccgaccc cgtctgcgac gcccggcttt acggcgcagc
    15481 tgccgacatg ctgctcacca tcgtgcgaga gctccccgac gacgcaggtt gcgcgatgct
    15541 gatcgggcac aaccccggca tggccgacat cgtcacgttg ctgaccggcg agtcgccgaa
    15601 gatgcgcacg tcggcgatcg cagtgctgcg cctcgccatg ggctggaaga aggcgagccc
    15661 gggcggggcc gcgctcgtcg cgcacgccac gccgcgcggc tgacacgctc gaccacgtcc
    15721 cgagccgtag tcagacctcc acccaagccc ggaagggcat gccggtacgg cgcgactcct
    15781 cgatcaccag acgcaccagc tcgaccatgc ccacgtagtc ctcgaaatcg aggaggaatt
    15841 ccaggtcctc gggcggttcc gcggtgaacg cacggcgctc ggcgaggtgc gcgccgaggt
    15901 cggtgaggaa ccggacgagg gcggccagct cgtcgtcacg gagctggaat tcggggtcgg
    15961 agtcgtaggc gaagtccccg gacacgcgct cgagctcggc tgccgcccgg gagtcgagca
    16021 gggcagcctc cgtcacgacc aactgccact gtggcgccgg caggacgaca gcccgacccg
    16081 caagccatgg cagatctgtg gcggtcagga gctcatcgtc cggcccggga gccacgaaca
    16141 cctgcgacgc gccgaccatt gcgcacctcc ccgtcggatg agcatcctcc cgcttgccgc
    16201 tggttcaccg gtcctccgcc tcgatggcgg cgacgcgacg acggataccg gcgatgaccg
    16261 cgtcgaccag ttcgtcgcgc gtcaggtcga tctggtgacc tctggccctc ggtcccgcgg
    16321 gcgtcatcat ctcgatcgcg atgtcgcccg cgtcggcgcc gcgcaccagg agacggcgga
    16381 actcggccag cgggatggcg ccgtcggagc cgtcccagga gaccccgacg tcgacgatct
    16441 tcgccagcgt ggccgggttc tgccgcaggt aggccaggta cgcccgcagt gcctccgggg
    16501 actccgtggg gatctcggag aacagccgtg cgagcttgac ccggtcctct gccggcgtga
    16561 acttcttcgg gttggccttc gcgctcacgt accgaaccgg tccggcggcg tcgaagaaca
    16621 ggaagtcgaa ctcgaccagg tcgcccgcgc tgccgccgga cgcggtcacc ggccgcgcca
    16681 ccacgttctt cagcacctgc tcgccgcgct ccgcggccag ccgctgcatg accggagcgg
    16741 caagggcctc cagcacgtcg ccgacgacgg ctgccaccca gctcacctca cgcgcgtact
    16801 gctgggggtc ccgcgtgaac ttggcgcggg tctgcggtgt cttcacgagc tgatgaccgt
    16861 gcttgcgtac ctcgcggtcg atgatctcct gctgctgtgc ggtcagggga cgcgtcgcgt
    16921 cggcgtcgtc cgcgccgacg cgcgccggac cggcgcctgg cgggatcgac gcgggcgtgc
    16981 tcgggtgggt cgcgaggaac tgaccgtcga tcggggggaa caccgacgtc agcaggtcat
    17041 catccgaacg agcagaggac ctcgtgacct tgaaccctcg tgacgggttc cccggcacca
    17101 gatcaaggag atcacgcacg acggcgttgg gcgcgggcca tgtgccggcg ttgcggagcg
    17161 ccgtggcgcg gttggcgacc caggaccggg cgagcgcgag cagccgctgg gcggtccggg
    17221 tgccggtcgg acccgtagcc gccgccgggc caccctgctc gggacgcgag tagcgctgtc
    17281 ccgcgatcgg gtcgtcggtc acgacggccg ggggtccacg ctgcgaccac gcgtgcgtcc
    17341 aggtcgtgac ccgaccggcc gcccttcggg ccgctccctg caccgacccg atccgggccg
    17401 tcacggctgt cgcgactccg gctccgatcg caacgacgac cggggccggt ccggcgagca
    17461 cgtacagagc cgcaagcgag atggcgacgg cgccgaacgt gaacagggcg cccaccgtgc
    17521 tccggcccgc atccccgccc ggctgctcgc tgtcgtccgt cgagcctccg gacggtcggg
    17581 ccgggtcggg gaaccggatt ccgtggtggt ggaaggcggc aagggcgatg gtgtgcagac
    17641 cctggcccgg ggtggcggtg ccgtggccgc ggtagtgacg ggtccggaca tcgatgctac
    17701 ggccgacgag gcgggcgcca tcgatatcga gcaccacctg tcctgcggcg agcacgtcgc
    17761 ggccctcgga cagcatcgga tggctgatcc gggccggtct gcgtcccggt ccgcgcactc
    17821 gcgccggggc gacccagatg gagccgtcgg tacggagcac ccagatgagg agcgggtact
    17881 cgcggatgga gtggcgtccg gccgtggtcc cgagctgcgc aggcgccacc ccggcgcgca
    17941 gggcagccgc gaggtgtccg gcaagcctcc agggccgccg gagcgggtac tgccgagcac
    18001 cggagacgag ttccgtgtag gcggagccgg tcaacatggt gcggggtggg ccccgggtcc
    18061 gcagtgccgc gacgagccag cggatgacct gttcccggtc cacccgcgcc aggcgggcac
    18121 cccaacggac cgacctgccc gcccggatcc cggcgatggc cgcgtcacgg tccctgatgg
    18181 gccgcaggtt cgcgtcggtc gcggcgacgc gggccttctc gaccagcgtg acggtcgccg
    18241 tcgtcgccgc cccgaccaca gcgacacctg ccgccaccgc agcaaccagc gccggcccac
    18301 ccagtccggg cacggcctgg tgcacgccga ggcccaccgc ggcgatggtc gtcgacaacg
    18361 acagtgcacc ctgccgggtg agcagggccg cacggacgcc ctgaccggtc gtgacgtacc
    18421 ggtatgcggt ggtcacggtg gttctggcga ccgcaagcgc ggccacagcc accaccggca
    18481 ccagcagggg gacgtcggcg agcaggggtc gtggagcggt catcaggccg agcccgatca
    18541 acgacagcgc aaaggtcgtg gtggcggtgg gcagcctgcg ggcggcgcgc acccccagca
    18601 cgagttcggt ctgcagcccg agggtggtca gatagccggt ggagccggtc aggaccgccg
    18661 acgcaacgac cttcaccgac aggacggcac cggttgcggc cgcagcaccg gacacgaccg
    18721 cggccgcggg caggttctgg gtcatgaaca gggcggtcaa cccgacgttg gcgagggtga
    18781 ccacggggaa ggccaagtag ttgttcagcc tgcgcgccca cggccaggac gcgatgttga
    18841 acctgaacca gccactgacc gcggtgggca gcgcgatggc catgaacagc aggtccgcac
    18901 cgacgtagaa cccgttggtc acggtctcga gccactgatc gcccaccttg agaccgacgt
    18961 aggtggcgtg catgtggtga gccaggttcc cgacccaggt caccgcgacg gcggcactga
    19021 tcgcccggcc ccaccgggcc tgcgccccgg tggactgcag tgaggtgata ctcgggccga
    19081 tggcccgggt ggacccgtcg ctccaccgcg agtagtccgc gatgacgcgg ccgagccggt
    19141 cccgcagcgt ctccccgtcc tgcaacggtt tgcgtagcag gcccttgtcc cggtcgtaca
    19201 tgttggtcag gaactcgcgg gtgacccttg tgaactcggg cagcctctgc tcgtcgaccc
    19261 cgtaggcccg ggcgttcttg accaccattc ggtacagcca ggtgatcttg gcacgggtga
    19321 tccgaccgcg ttccatcatg gagtcgtgca cttcccgcac cgcgctctgc tcggcggtct
    19381 tgagcagggt tgcggcgccg cgcacgacga acgcgatcga actgatccac gggagcgcgg
    19441 cggggatcag ccacagcccc gcgagcaccg cgcctgcggc caccgcggtc accccggccg
    19501 tccaggcccg gaacgctcgc cggaccaggc cggccttcgc cgcgatcacc tccctggcgt
    19561 ggttcacctg ttcctggcgc tcctgcacca actccaacgc cacccgctgg tgcacgccgg
    19621 acgtgccgac ccgctgcgcc tggtcggcca cgatcagggc ctccagcgtg tacgacccac
    19681 cggggaggtc ctgcggcgca accgccgcct cgaccgccgc ccgggcgtca gccggctgct
    19741 gcaggcccga cccgtgtccg cgccgccact cccgcgctgt cacccgcacc ccgtgccgcc
    19801 acagccgcag cgtctgcctg ccgctggccg aacgttcgtc gaccgcgcgg taccagccca
    19861 gcatgtccgc caactgctgg tcgtcgaacg gtcgcggacc cggatcgtgc accggagcag
    19921 gtcggggtga ggagcccggc gtccgagccg tctgggtcgt gccgacgccg tacaacctct
    19981 gcagggccac ccagtcacgc acgcgttgcc ggaccatgcc cgggaaggcg cgcaacaccc
    20041 cgtcccacac gccggatccg acattggcga gcgtccacgc ccggacgtcc cgacgggcca
    20101 ccgtcaacaa cgcgatgagg ttgcgataac cgatgcgctc gtacgcgccc ggggcgagcc
    20161 gatcgacatc ggccaggatg ttcgccatgt cccggtggtg gcgcaggtag tgcagaacgg
    20221 tggcgggctt cacgccgtcg gttccaagga tgaagaaccg atgaaaccgg gaaccgagcc
    20281 gcacgaaatg ctgacgcagc gcaggagttt cctcgatgtg ctgggccagg tcaccccagg
    20341 aaaggtcggc gtggaggttc ggatacttct cggtccgcac ctcgaacacc tctttctcga
    20401 ggaagtccag gtactcgcgc gagagcgtgg tgaactttcc ggtgccgatg tgcgccagga
    20461 tcacccgcgc ctccgggaag tcccgcagag cggcgagaaa agcgatcagg taacgccagt
    20521 cgggctttcc ggcgatcggg aagccgtcct cgcccagcaa cggctccccg gcatcgttgt
    20581 gcactacgcc aatcgccccg ctcgcggtca ggaactccag gtagtcgacc agaccctgcc
    20641 cgtagacccg cccgatccgg taccggccca gcccctcgcc ctccctgagc actccgagca
    20701 ccgtcagcgc gtcgcgcagt tcccacagcg cagctgggtc ggcgaccgtc gggtcggcga
    20761 gttcgtgcag cttcctgacg atccgcaacg cgaccttccc ggcggccgcg gagtacaggt
    20821 cgtcgacgac agcagacagg cgggggaagt ccgcccggtc gaggcggccg tcggcgaacg
    20881 ccgcccggag caggggcacc actgcgcgca ggcgttcgag cgtccggaca tatcccgcat
    20941 ccagcaggtt cgccgcctga ggcccgagct ggatcttgac gttctccttg ttgaacgtct
    21001 tctcgcccag caggaaggcc agacccgcat tcacgagcgt cgtcaccatc agatatatct
    21061 tcaggcccgg aatatcgaag cgaccccccg tgataccggc gaccacccgc caggcgaggt
    21121 gcgcgggaag cgcccggatc gccgcgagga catgcgcatc ggtgagcttg ccgcgcatcg
    21181 ccagcatccc gacgccgaac cgggtcgtgg ccacctgcgc gtccacgtcg ccgtagtacg
    21241 ccaagccctt gatgcgctgc gggatcggat ggaaggtcag caccacgtca taaccggcgt
    21301 ccagcatctt cgcgaccagg ccgccgagcg acgtggcccg gtgatagggg taaccggaat
    21361 ggtgtagatg cccgtcggcg aacttccgca acgcgacgac ggcctccgag agacgaggat
    21421 ccaccccacc ctccgggaat gccagccgca cccggtcacc gcgcccggtt actttcagcc
    21481 ccaaggaccg catctgcgac aacgccggaa ggatcaacca gtcggtcacg atgggcggcc
    21541 cggccgccac gtcccggcca tcagcctccg gctcgatcct cgccaccagc tcattggcct
    21601 tctgcaggcg gacccggcgc tgctccgtga gctcgaccaa tttcccgatc aacgcgtcgg
    21661 ggctgagccg cccgcccacc acacccggcg gggcacgcgg cgacgcgacg gagctcgcca
    21721 cgatcgaacg gacctcgacc tcatcgagcc tcaacgccga tgccagctgc tcgtggatcc
    21781 agtcgtcgtc gcgccgtccg tggagctgat cgaacgcccg cccgacccac gctcgacgct
    21841 ccgaaaatcc cagggacgcc tgcatgtgtt ccagtcgagc cgcgccagga ccgtccccgg
    21901 tacgaccgga tcccactgcc gcgcgccgta cccgaccggg ccagcgcggc gcgcgacccc
    21961 acgatccacg tgcggcagga ggtccgcgcg tggctctccc ggtgagcacc ccgatcagcg
    22021 caggcggcac ctccagagca cgggcgatcc gacgttccgg cgctcccccg gccaccgccc
    22081 gcgcgatcgc ggcaccgacc agatcccgca ccgcgtcgcg agccgcggag tggagctcgt
    22141 ccgccttccg caacccctca cgacgtgtcg cggtctccgt ggcgatcgcg tccaggtact
    22201 tccgaccggt gaggttgaag tactcgatgt ccggcgccgc ctgccaccga tcggtcagca
    22261 cccgctcgtg aacctcctcg cgcgcgatcc cgagcgcgtc ggccgccagc tggacgatgt
    22321 cccgccccgc gaagccgggg tccttcgtca gctcctcgac caggcgagcc ggccaaccga
    22381 accgctgcgc gtacatcccc cgcgccaact cggccacccg cctggtctcg atcaggtcgg
    22441 tgaccgcacc cgagagctgg tcgcccatgc cccgcaccgc ggcgcgcaga gcacgagtgg
    22501 ccaggtacgc cgcgggcgtg gacgcggcgc cgacccgggt ccgcgccgac cggaccccac
    22561 ccgccaccgc ggcgacctgg agccgcgcca ccgcacccca ccacgcccga ctcaccagga
    22621 gtgggcgggc ggagatccgc acgcccagtc cgaactccag tccggacacg acccccgacc
    22681 accacgcccg gaggccgcgc ccccatgcgg cgaggacggc acgcggcggc gccctggcct
    22741 gccgcggcgt ccaccacccc cggaccccaa ccacgaccgc gccgaccgcg accgccaccc
    22801 cggccgccgt gagacctgtc gcaccgcccc accagccggc ggccaccgag ccaccggtgt
    22861 ccacagtgcc gccccgggtg ccggcctggg cgacagcggc gccgaaccag tccgagacca
    22921 ccgcacccgt ggtgaggaga cccacgacca ccggcgcacg cccgagcatc cgcagtgctg
    22981 cccgcagacg cagtgctgcc cgcagacgct tcgcacggag gtcggccgcc cccgtcaacc
    23041 gcgaagccgc ctccggccgg ctgttccggg caccctgtgc cgggaccgcc gtgccggaac
    23101 cgccgggctg cagggcgttg ccggagcccg atgcaggcga tcggggacca cccgaccgcg
    23161 gcggcccgga tatgccggta cctccgccgg cgctcggccc gcccgatggg ccggagctgc
    23221 cggatgtcgg gttctcggga gcaccagagg ctgggggtcc cctggtgcgc cggctacgcc
    23281 aggtgtcgag cttcgacagc gcccaggttc cggcgagcag ggccacggcg ccgacgctga
    23341 acccgaccca ctggacaggc agcaggctca tcgcgccgaa cgccagcagg ctgccggaga
    23401 gaatggcgtt cgcgccggca cccctgcggg gcgcgggacg cccgtggccc agctcgatgg
    23461 tgatgccaag ggcggacagg taaccgcccg ctgcggtcag cccgagcgcc ggcaccacca
    23521 tgatgctgtt gtcgatcacg ccggtctgga tcaccagcag caggctgccc aacgtcaccg
    23581 cggggacggc ccccaggtgc acgaccttgc ggagcaccgg gtggctactg aagtcgatgc
    23641 ctgcccaccc catgaccgtc acggacagcg cctgcaccag gaacagcgcc gacgagagcg
    23701 cgaacgcacc gttgacccag gcgtcgaggc cggtcccgtt caccatcgcc ggcgcctgca
    23761 cgaccaggtt gaccaggtac gtgccggcca gcacggtgtt gaccacccgg cccagcagcc
    23821 cggcgttcgg ggcgaagccc tggaaggact gcgcctgagc gcccagctcg acagccgcct
    23881 tgtcgaggaa gagcgagaac tccttgagtg ccgcgtgact gcggccctgc gcagtctggc
    23941 ccggccggag cggggagaag accagagcgt gcgcgatcac caggaagtca ttggcgacct
    24001 tgtcgaaacc cgccagctgc gtcggagtcg acccgtcgag tgccgcgtac ttgcgcatca
    24061 cgctgatcag cacgcggatc gtgtcgatgt cgaactggcc gcgttccatg atcgactcga
    24121 ccagcacacg cagctcctgg ctgtacgcgg tgcggtgcag acccagcgcg ccacggacgg
    24181 cgaacgcggc gtagtgcgcc gacgccgaca tgggcagcaa cgcccacgga gcgaccgtca
    24241 ccgcggccgc gccgagcccg accatcacgc cggcagcgac gatcgccttg accgtgtagg
    24301 tcccgcgcaa ctccttccgc ccgccggcgt cccgggtctg tgcgagctgg gtgcgctgca
    24361 cctcgcccaa cacccacgcg gccctggcct ggtcacgcat cgcgccgctg gtctggtgag
    24421 cggcgaccag cgcctccaac gtgtaggcgt tgccgtcggc gtcgcggaga tccagggcgg
    24481 ctgtgtccgt ggcgccaccc agaccacctt cggcgcgggc cgcctgcttc gccgcgcgcc
    24541 gatcggcccg gtcgccgcgg cggtccgccc agatcgcacg cagcgacgcc atgaccatgc
    24601 ggggggtcag cctgcgcccg tccaccactc cgggggtcac cgcgttgtgc cagcggatca
    24661 ggctgagcat ctgccgggcg gcgggaccgg tctcctccca ccaccggccc gggtcgggct
    24721 ttccgagctc ggccagggtg cgccgcagcc cgtcgccctg ttccagctcg tagcgctcac
    24781 gccagtcgtc cacgatccgg ttgcgctcag cggagaacgc agccctgcgc cac
//
LOCUS       DASRSC010000137         4490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2830741,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000137 DASRSC010000000
VERSION     DASRSC010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2830741"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VFE39_06570"
     CDS             <1..657
                     /locus_tag="VFE39_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6258298.1"
                     /translation="VLGEGGYVPTPPAFLVGLRERADAHGILLVADEVQTGFGRTGRF
                     WGHEHTEGVTPDILITAKGLASGFPLSGIAAPTELMAKAWPGSQGGTYGGNAVACAAA
                     LATLDVIKDEGLVANAAEQGVRLLEGLRKVAADHPGIADVRGLGLMVGNEFCTPDGAP
                     DTAAATRAHAAAAEHGLLLLTCGPYANVVRMIPPLIVTAEQVDEAVRLWAEAVRTAVR
                     "
     gene            complement(804..1952)
                     /locus_tag="VFE39_06575"
     CDS             complement(804..1952)
                     /locus_tag="VFE39_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HET6258299.1"
                     /translation="MSTTGNTTTTEQAVRAALATVDDPEIHKPITDLGMVKSVVVSPE
                     GRAQVGVYLTVAGCPMRDTITKRVTEAVSAVAGITGVDVELDVMSDEQRTELRRGLRG
                     DAAEPVIPFAQPGSLTRVYCVASGKGGVGKSSITVNLAAAMAARGLSVGVVDADIYGH
                     SVPRMLGTTARPTKVEDMIMPPQAHGVKVISIGMFTPGNDAVVWRGPMLHRALQQFLA
                     DVYWGDLDVLLLDLPPGTGDIAISTAQLVPNAELLVVTTPQTAAAEVAERAGSIALQT
                     RQRLAGVVENMSWMELPDGSRMEVFGSGGGQTVADSLTRILGAPVPLLGQVPLETRVR
                     ECGDSGTPIVLAEPEAPAAVALRAVANKLTTRARGLAGMSLNISPVSR"
     gene            2063..2551
                     /locus_tag="VFE39_06580"
     CDS             2063..2551
                     /locus_tag="VFE39_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6258300.1"
                     /translation="MTDTAAHAPPADSPAPATRSVPTREQLVSWRAFLRAHATIIRAL
                     ETELVAEQNISLAAYDVLLQLAEAPGRRLRMTELADAVLLSRSGVTRLVDRLEKSGLV
                     ARCPVENDGRGVAAELTEAGLNRLRIASRTHLNGIVRYFVAKLDAEQLGELEQISRRL
                     AE"
     gene            complement(2529..3059)
                     /locus_tag="VFE39_06585"
     CDS             complement(2529..3059)
                     /locus_tag="VFE39_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HET6258301.1"
                     /translation="MPENTTHRRLDQPRLGRARLEMDPDWFPRLSERIARYLGTGRFL
                     AIQTVIVIVWIALNLTAVIGRWDPYPFILLNLAFSTQAAYAAPLILLAQNRQDDRDRV
                     SLEEDRARAARTKADTEFLARELAALRIAVGELATRDYLRGELDKLAEKICAERPGTG
                     PDTDRARTRVIRPADG"
     gene            complement(3052..4359)
                     /locus_tag="VFE39_06590"
     CDS             complement(3052..4359)
                     /locus_tag="VFE39_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6258302.1"
                     /translation="MSTDRVFVARLAGMAVFGPDGERIGKVRDLVASLRVDASPPRVL
                     GLVVELATRRRIFVPMLRVTSIDPGAVTLATGSVSLRGFAQRPNETLLVGQLIDARVR
                     LVESGEEVVVVDAAIEPTRSRDWVVGRLALRARRHGLSRRGPVQVVPWNAVTGLSLSD
                     RQGTAGLLAVFETMRPADVAAALSELPEKRQHEVVDSLDDERLADVFEEMSESDQRKL
                     LEHLNAERAADVLEAMSPDDAADLLHELPDADADALLALMEPGESGPVRRLMSYSSDT
                     AGGLMTPEPIILRPDATVAEALARIRNPDYPPALASMVFVCRPPSETPTGRYLGCVHT
                     QRLLREAPFDLVAGVVDTELARLTPDATLGEITRYFAAYNLVAGPVVDSEDHLLGAVS
                     VDDVLDHLLPPDWRDRLTEPEGVPDREPDHDANPDANPEVADA"
BASE COUNT          625 a         1600 c         1623 g          642 t
ORIGIN      
        1 gtgctcggcg agggcgggta cgtgcccacg ccgcccgcgt tcctcgtcgg gctgcgcgag
       61 cgggccgacg cgcacggcat cctccttgtg gccgacgagg tgcagaccgg cttcgggcgc
      121 accggccggt tctggggcca cgagcacacc gagggcgtca cgccggacat cctgatcacg
      181 gcgaagggcc tggccagtgg cttccccctg tcgggcatcg cggcccctac cgagctcatg
      241 gcgaaggcct ggccggggtc gcagggcggc acgtatggcg gcaacgcggt ggcctgcgcc
      301 gcggcgctcg ccacactcga cgtgatcaag gacgagggcc tcgtcgcgaa cgccgccgag
      361 cagggcgtgc ggctgctcga gggactgcgg aaggtggcgg ccgaccaccc gggcatcgcc
      421 gacgtccgcg ggctcggact catggtgggc aacgagttct gcaccccgga cggcgcgccg
      481 gacaccgccg cggccacccg cgcgcacgcc gcggctgccg agcacgggct gctgctgctg
      541 acctgcggcc cgtacgcgaa cgtggtgcgg atgatcccgc cgttgatcgt caccgcagag
      601 caggtggacg aggccgtcag gctctgggcg gaggcagtgc gcaccgctgt gcggtagggc
      661 gagcggatct cgcaacttcc gagcgcccag cacgcgatga ccgctgccct gcccgacgcc
      721 tggctcccgc ctggcgggga ttctcggcgg tgcggtaccg ctgatcgtgg tgagtgctcc
      781 gcactcgtac ccgtgcgcga gggctacctg ctgacggggg agatgttcag gctcatcccg
      841 gcgagcccgc gggcccgagt ggtgagcttg ttggccacgg cgcgcagcgc aacggccgcg
      901 ggtgcctccg gctcggccag cacgatcggg gtgcccgagt cgccgcactc gcgcacgcgg
      961 gtctcgagcg gcacctgccc cagcagcggc accggagcac cgaggatgcg cgtgagcgag
     1021 tcggcgacgg tctgcccgcc gccggaaccg aagacctcca tgcggctgcc gtcgggcagc
     1081 tccatccacg acatgttctc gacgacgccg gcgagccgct ggcgggtctg cagcgcgatc
     1141 gagccggccc gctcagccac ctcggccgcg gccgtctgcg gtgtggtgac caccagcagc
     1201 tccgcgttcg gcacgagctg ggcggtggag atcgcgatgt cgccggtgcc gggcggcagg
     1261 tcgagcagca ggacgtcgag gtcgccccag tacacgtcgg ccaggaactg ctgcagcgcg
     1321 cggtgcagca tcgggccgcg ccagacgacc gcgtcgttgc cgggggtgaa catcccgatc
     1381 gagatcacct tcaccccgtg cgcctggggc ggcatgatca tgtcctcgac cttggtgggc
     1441 cgcgcggtgg tgcccagcat ccgcggcacc gagtggccgt agatatcggc gtcgaccacg
     1501 cccaccgaca gcccgcgggc ggccatcgcc gcggcgaggt tgaccgtgat cgacgacttg
     1561 ccgacgccgc ccttgcccga agccacgcag tagacacggg tgagcgagcc gggctgggcg
     1621 aacgggatca ccggctcggc ggcgtcgccg cgcaggccac gacgcagctc cgtgcgctgc
     1681 tcgtcgctca tcacgtcgag ctcgacgtcg accccggtga tgccggcgac ggcggagacc
     1741 gcctccgtga cgcgtttggt gatcgtgtcg cgcatcgggc agccggcgac ggtgaggtac
     1801 accccgacct gcgcacgacc ctcgggcgac accacgacgc tcttcaccat gccgaggtcg
     1861 gtgatcggct tgtggatctc cgggtcgtcg acggtggcga gcgctgcccg gacggcctgc
     1921 tccgtggtgg tggtgttgcc ggtggtggac atcagagtct ccgctgcacc ttcctgcgac
     1981 tttactagga cgcgaccgcg ccacgacctt ttccatgcta cgtctgtgcc ctcgcgggct
     2041 tacgtccgta gcatcgtccg ccgtgacgga caccgccgcc cacgcaccac cggccgactc
     2101 cccagctccg gcgacccgga gcgtacccac gcgcgagcag ctcgtctcgt ggcgagcgtt
     2161 cctccgggcg cacgccacga tcatccgcgc tctggagacc gagctggtgg ctgagcagaa
     2221 catctcgctc gccgcgtacg acgtgcttct ccagctcgct gaggcgccgg gaaggcggct
     2281 gcggatgacc gagctggccg acgcggtgct cctctcgcgg tccggcgtca cgcggctcgt
     2341 cgaccggctg gagaagtcgg gcctggtggc gcgctgcccg gtggagaacg atgggcgcgg
     2401 tgtcgccgcc gagctcacgg aggcggggct gaaccggctc cggatcgcct cgcgcaccca
     2461 cctcaacggc atcgtgcggt acttcgtcgc gaagctggac gccgagcagc tcggtgaact
     2521 ggagcagatc agccgtcggc tggccgaatg actctggtcc tggcccggtc ggtgtccggc
     2581 ccggttcccg gccgttctgc gcagatcttc tcggcgagct tgtcgagctc gccgcgcaga
     2641 tagtcacgcg tcgccaactc gcccaccgcg atccgcaggg ccgcgagctc ccttgcgaga
     2701 aactccgtgt cggccttggt ccgcgcggca cgagcgcggt cctcttcgag cgacacccgg
     2761 tcgcggtcgt cctggcggtt ctgagccagc aggatcagcg gcgcggcata ggcggcctgc
     2821 gtggagaagg ccaggttgag caggatgaac gggtaaggat cccaccgccc gatcaccgcc
     2881 gtcaggttca gcgcgatcca cacgatgact atcacggtct ggatcgcgag gaagcggccg
     2941 gtgccgaggt accgggcgat tcgttccgac agccggggga accagtccgg gtccatctcg
     3001 agccgggcgc ggccgaggcg cggctggtcg agcctgcggt gggtggtgtt ctcaggcatc
     3061 cgccacctcg gggttcgcat cggggttcgc gtcgtggtcg ggttcccggt ccggcacacc
     3121 ttcgggctcc gtcagccggt cgcgccagtc cggcgggagc aggtggtcga gcacgtcgtc
     3181 cacgctgacg gcgccgagca gatggtcctc gctgtcgacg acgggccccg ccaccaggtt
     3241 gtacgccgcg aagtaccggg tgatctcgcc gagcgtcgcg tccggggtca gccgcgcgag
     3301 ctcggtatcg accacgcccg ccaccaggtc gaacggcgcc tcccgcagca gccgctgggt
     3361 gtgcacacac ccgaggtagc gccccgtcgg ggtctccgag ggcggccggc agacgaacac
     3421 catgctggcc agcgcgggcg ggtagtcggg gttgcggatc cgggccagcg cctcggcgac
     3481 cgtcgcgtcc gggcgcagaa tgatcggctc cggcgtcatg agcccgcccg cggtatcgga
     3541 ggagtagctc atgagcctgc gcaccgggcc cgactcgccg ggctccatca gcgcgagcag
     3601 cgcgtcggcg tcggcgtcgg gcagctcgtg cagcaggtcg gccgcgtcgt cgggggacat
     3661 cgcttcgagg acgtcagcag cccgctcggc gttgaggtgc tcgagtagtt tgcgctggtc
     3721 tgactccgac atctcctcga acacgtcggc gagccgttcg tcgtcgaggg agtcgaccac
     3781 ctcgtgctgg cgcttctcgg gcagctcgga gagcgcggcg gcgacgtcgg cggggcgcat
     3841 cgtctcgaac accgccagca ggcccgcggt gccctgccga tccgaaagcg acagcccggt
     3901 gaccgcgttc cacggcacca cctggaccgg tccgcggcgg gacagcccgt gcctcctcgc
     3961 gcgtagcgcg agccggccga cgacccagtc gcgcgagcgg gtcggctcga tcgccgcatc
     4021 gacgacgacg acctcctcgc cgctctccac cagccggacc ctggcgtcga tcagctggcc
     4081 gacgagcagc gtctcgttcg ggcgctgggc gaaaccacgc aggctgaccg aacccgtcgc
     4141 gagggtcacg gcccccgggt cgatgctggt cacccgcagc atcgggacga agatccggcg
     4201 ccgggtggcc agctcgacca ccaggccgag cacacgcggt gggctggcgt cgacgcgcag
     4261 cgacgccacg aggtcgcgaa ccttgccgat gcgttcgccg tcgggcccga acacggccat
     4321 gcccgccagc cgcgcgacga agacccggtc ggtgctcacg cagaaagcct aggctgcagc
     4381 cgtcaattcg ccccgatacc acgtggtggc ggcacacggg acgtgtcgcc gcccgcctca
     4441 ggagcgagct gaacaactcg gccctgctgc gcggtgatcc aggcggcgtt
//
LOCUS       DASRSC010000138         6533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2175123,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000138 DASRSC010000000
VERSION     DASRSC010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6533
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2175123"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="VFE39_06595"
     CDS             <1..939
                     /locus_tag="VFE39_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6258303.1"
                     /translation="GAMIGRLAGLGVGAGFTAAAVLGSDPAVPPAERWLAGVWWTGYL
                     VAAAVLVGVLLRMLERQLDAAAVSRARALAEHEAFLEERDLRARLLAAQQARMDGVRV
                     VLHEFRTPVASLTALTADLASDRLSGDARSTATRLLAEHAVHLRDMLDGLADVAVQEG
                     SPLGRVRERPVRLGELAEAVLDAAGVEPARRAHRVTPPDAVVRCDPQRLRRVLTNLVE
                     NAARHSGDEVVELELRHADGRLVAEVRDRGPGLPPGQAGVVTAKGVALGDRRGTAGLG
                     LWIVEALVAAMDGELRLLPRDGGGLVAHLELPVPAT"
     gene            1038..2144
                     /locus_tag="VFE39_06600"
     CDS             1038..2144
                     /locus_tag="VFE39_06600"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HET6258304.1"
                     /translation="MKTTAAVLRGVGGKWEVEEVELDPPGPGEVLIELVASGLCHSDE
                     HMVTGDLPVAFPVIGGHEGAGRVLQIGPGVTDVVTGDPVVLTFLPSCGRCSYCVRGHT
                     ALCDDGAGAMLGPQLDGTYRFHARGEDLGQMCLLGTFARHTVVPVKSVVKIDEGIPLE
                     LAALVGCGVTTGVGSAIRAAELHPGDTAVVIGVGGIGANAVQGAKLAGCRYVMAVDPV
                     EFKREKALELGATHVAANMDDAWQTVSELTRGRLADAAILTTGVAEGSYLQPALQLVG
                     KRGRVVVTALGHPSEDTASLSLLELTLYEKQIRGSLFGSSNAQHDVPRLLELYQLGQL
                     KLAELVTRTYALEEINTGYQDMREGRNIRGVIHY"
     gene            2194..3168
                     /locus_tag="VFE39_06605"
     CDS             2194..3168
                     /locus_tag="VFE39_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="R2-like ligand-binding oxidase"
                     /protein_id="HET6258305.1"
                     /translation="MTDERSIEHGRRAGFATLQRGGLNFDSFPMRLFAKGNRRHWNPA
                     DIDFTQDARDVAEMTELERWWTCSLAAQFMAGEESVTQDLQPFVAAMAAEGRLADEMY
                     LTQFVFEEARHTEAFRRWFDAVGLTDDLHSYVESNHHYMEIFTEQLPNSLHALAADPS
                     PLTQIRASVTYNHIVEGTLALTGYFAWAKVCSSRGILPGMQQVIKLIGDDERRHMAWG
                     TFTCRRHVAADDRLWDAVDQRMQELLVPAMGVVTHLYEQFEGEDPPFGMDVNELAEYA
                     MDKVGRRLGAIESARGADLRVIDVDTAPEQLEERFHAEDQAELPVVLA"
     gene            3425..4639
                     /locus_tag="VFE39_06610"
     CDS             3425..4639
                     /locus_tag="VFE39_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6258306.1"
                     /translation="MTGVEVKVEGLTKSFGRANIWSDVTLTLPPGEVSVLLGPSGTGK
                     SVFLKTLIGLLKPERGSIVIHETDLVRCSESRLYELRKLFGVLFQDGALFGSMNLYDN
                     IAFPLREHTKKSESEIRDIVAEKMAMVGLTGDERKLPGEISGGMRKRAGLARALVLDP
                     EIILFDEPDSGLDPVRTAYLNQLIIDLNAQTDSTFLIVTHDINTAQTVPDNIGMLYRK
                     HLAMFGPREVLLTSEEPVVAQFLNGRRQGPIGMSEEKDTAQAAREMEEAGELAGLPPM
                     KPQLQPSPGVGERKAVGRRRERVQQMLHTLPAAAQQAIRASYADDPLPAPRPVGPPAR
                     PASSWRMGRRSPARTDDSVPGYPPRAPGYGPEADSFDTFDQGYGGGDPAGGPGSGPGS
                     PPPPSGPGRHHI"
     gene            4650..5429
                     /locus_tag="VFE39_06615"
     CDS             4650..5429
                     /locus_tag="VFE39_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6258307.1"
                     /translation="MTSPAPLTRALTPVGRLFGLGVAVVRNTFRLPFQLSEYIEQTWF
                     VTKVSALPTALFTIPFGATIALLLADLTQQFGAQSQTGAGSVLAIVQQAAPIVTALLI
                     SGAGGSAVCADLGARTIREEIAAMEVLGISPIQRLVVPRVLAMATTAVVLNGLATVVG
                     VAGGYFFNVVVQGGTPGAYIASFSAIAQVSDILVSEIKAALFGFTAGVVAAYRGLNPP
                     PGPKGVGDAVNQAVVISFVLVFLINLVLTSLYLEFVPAKGS"
     gene            5431..6270
                     /locus_tag="VFE39_06620"
     CDS             5431..6270
                     /locus_tag="VFE39_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6258308.1"
                     /translation="MALLNGRARQVVNAPLQVLEELGEQLSLYARAIVWIPRTLRRYR
                     SEIARLLAEVSFGSGALIVILGTAGVMLSLSLFVGSLVGLQGFRALDSLGVDALTGFI
                     TAYFNTRDIAPLVASAALTATLGAGFTAQLGAMRISEEIDALEVMAVPSVPFLITTRV
                     IAGVIAIIPMYTIGLLASFAASRLNVTLINNLPGGTYDHYFNLFLPVSDVLYSYLKVI
                     IFAMVIILIHCHYGYNAKGGPAGVGVAVGRSVRLSIVAAAVLDFFLTLVIYGTDTSVR
                     VAG"
     gene            6267..>6533
                     /locus_tag="VFE39_06625"
     CDS             6267..>6533
                     /locus_tag="VFE39_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HET6258309.1"
                     /translation="MKAVRGLAFLGVLVLLAGLAVAQYAGAFRSGVPVTLKVARVGTQ
                     LDPKADVKVRGLIVGSVTSISTDGAGATVALSLDPRMVNEIPADV"
BASE COUNT          933 a         2246 c         2360 g          994 t
ORIGIN      
        1 ggggcgatga tcggcaggct cgcggggctc ggggtgggtg ccgggttcac tgccgccgct
       61 gtcctcggat ccgacccggc agtgcctccc gccgagcggt ggctcgccgg cgtgtggtgg
      121 acggggtacc tggtcgccgc cgcggtgctg gtgggtgtgc tgctgcgcat gctggagcgt
      181 cagctcgacg cggcagcggt gtcccgcgcc agggcgctcg ccgagcacga ggcgttcctg
      241 gaggagcgcg acctgcgggc ccgcctgctc gccgcccagc aggcgcggat ggacggcgtg
      301 cgcgtcgtcc tgcacgagtt ccgtacgccc gtcgcgtccc tcaccgcgct cacggccgac
      361 cttgcctccg accgtctctc cggggatgcc cggagcacgg ccacccggct gctggccgag
      421 cacgccgtcc acctgcgcga catgctcgac gggctcgccg atgtcgcggt gcaagagggc
      481 agcccactcg gacgggtgcg ggagcggccg gtgcggctgg gggagctcgc cgaggcggtg
      541 ctggacgcgg cgggcgtcga accagcacgg cgggcacacc gcgtgacacc gccggacgcc
      601 gtggtccggt gcgacccgca gcggctgcgt cgggtgctca ccaacctcgt ggagaacgcc
      661 gcccggcaca gcggcgacga ggtcgtcgag ctcgagctgc ggcacgccga cgggcggctc
      721 gtggccgagg tgcgcgaccg cgggccgggc ctgccgcccg ggcaggcggg cgtggtgacg
      781 gccaaggggg tagctctcgg ggaccggcgg ggcacggcag gcctcggact gtggatcgtc
      841 gaggcgctgg tggcggcgat ggacggggag ctccgcctgc tgccccgtga cggcggtggc
      901 ctggtggcac acctggagct ccccgtcccg gcgacctgac ggcggccggg cccgcggctg
      961 ctggactggc accggcgggc cacggatgga acccgcggcc ggtatgggag tcgggagatc
     1021 cacgcgatgc tgatcacatg aagaccacag cagcggtgct tcggggcgtg ggcggcaaat
     1081 gggaggtcga ggaggtcgag ctcgacccac cgggccccgg cgaggtgctg atcgagctgg
     1141 tcgcgagcgg cctgtgccac tccgacgagc acatggtcac cggggacctg cccgtggcgt
     1201 tcccggtgat cggggggcac gagggggccg gtcgcgttct ccagatcgga ccgggggtca
     1261 ccgacgtggt caccggcgac ccggtcgtcc tgacgttcct gccctcctgc gggaggtgca
     1321 gctactgcgt gcgcggccac accgccctgt gcgacgacgg cgcaggcgcg atgctcggcc
     1381 cccagctcga cggcacctac cgattccatg cccgcggcga ggacctcggc cagatgtgcc
     1441 tgctgggtac gttcgcgcgg cacaccgtcg tgccggtcaa gtccgtggtg aagatcgacg
     1501 agggcatccc gctggagctg gccgcgctcg tcggctgtgg tgtcaccacg ggtgtcggct
     1561 cggccatccg cgccgcggag ctccacccgg gcgacaccgc ggtcgtcatt ggtgtcggcg
     1621 ggatcggcgc caacgcggtg cagggcgcga agctggccgg ttgccggtac gtgatggccg
     1681 tcgaccctgt ggagttcaag cgcgagaagg cactggagct gggcgcgacc cacgtggccg
     1741 ccaatatgga cgacgcgtgg cagacggtct cagagctcac ccgtggccgg ctcgccgatg
     1801 ccgcgatcct gaccaccggg gtggccgagg gcagctacct gcagcccgcg ctgcagctcg
     1861 tcggcaagag gggccgcgtc gtcgtcacgg cgctcggtca cccctccgag gacacggcgt
     1921 cgctgtcgct cttggagctc acgctctacg agaagcagat ccggggctcg ctcttcggca
     1981 gctcgaacgc acagcacgac gtgccgcgcc tgctcgagct gtaccagctc ggccagctga
     2041 agctggcaga actcgtcacc aggacctacg cgctcgaaga gatcaacacg ggctaccagg
     2101 acatgcgcga gggccgcaac atccgcggcg tcatccatta ctgatcccgg cagtactgat
     2161 actcgtagcg accacaccgc aaaggagctg agggtgaccg acgagcgatc catcgagcac
     2221 ggcaggcggg ccggattcgc gacgctgcaa cgcggcgggc tgaacttcga ctcgttcccg
     2281 atgcggctgt tcgcgaaggg gaaccggcgg cactggaacc cggccgacat cgacttcacc
     2341 caggacgcgc gggacgtcgc cgagatgacc gagctggagc gctggtggac ctgctcgctc
     2401 gccgcgcagt tcatggcggg cgaggagtcg gtcacccagg acctgcagcc cttcgtggcg
     2461 gcgatggcgg cggagggacg cctcgccgac gagatgtacc tcacgcagtt cgtcttcgag
     2521 gaggccaggc acaccgaggc gttccgccgg tggttcgacg ccgtggggct caccgacgac
     2581 ctgcactcct acgtcgagtc caaccaccac tacatggaga tcttcacaga acagctgccg
     2641 aacagcctgc acgcgctcgc ggccgacccg tcgccgctca cgcagatccg tgcctcggtc
     2701 acgtacaacc acatcgtcga gggcacgctg gcgctcacgg gctacttcgc ctgggcgaag
     2761 gtgtgctcca gccgagggat cctgcccggc atgcaacagg tgatcaagct catcggcgac
     2821 gacgagcgcc ggcacatggc atggggcacg ttcacctgcc gccgccacgt cgccgccgac
     2881 gaccgcctgt gggacgccgt ggaccagcgg atgcaggagc tgctggtgcc ggcgatgggt
     2941 gtggtcaccc acctctacga gcagttcgag ggggaggacc cgccgttcgg gatggacgtg
     3001 aacgagctgg cggagtacgc gatggacaag gtgggccgca ggctcggcgc catcgagtcg
     3061 gcgcggggcg ccgacctgcg tgtgatcgac gtggacaccg ctccggagca gctcgaggag
     3121 cggttccacg ctgaggacca ggcggagctc cccgtcgtac tggcctgatc aactgctgcc
     3181 gtacggacca cccggtcggg acatcccggc cgggtggttt cgcgtccgca ggcagacgta
     3241 gagcgaccgc cgtactccga ccggtcccca aatttccgcc ggatggagca acttttcgcc
     3301 ggaacatgac cggtgagtaa ccgacgtcat caggtctcgt tgagccaccg tgggcagccg
     3361 gccgctcccc gctccccgct cccctggccg gctcgctccc gacgagaagg ctcaggaggc
     3421 gccggtgact ggcgtagagg tcaaggtgga agggctgacg aagtccttcg gccgcgccaa
     3481 catctggtcc gacgtgaccc tgacgctgcc gcccggcgag gtgtcggtgc tgctcggccc
     3541 gtccggcacg ggcaagtcgg tgttcctcaa gaccctcatc gggctcctga agccggagcg
     3601 gggctcgatc gtcatccacg agaccgacct cgtgcgctgc tcggagtcgc ggctctacga
     3661 gctgcggaag ctgttcgggg tgctgttcca ggacggcgcg ctgttcgggt cgatgaacct
     3721 ctacgacaac atcgcgttcc cgctgcggga gcacacgaag aagtcggagt cggagatccg
     3781 ggacatcgtc gcggagaaga tggcgatggt cgggctcacc ggcgacgagc gcaagctgcc
     3841 cggtgagatc tccggcggga tgcgcaagcg tgcgggcctg gcgcgggcgc tggtgctcga
     3901 cccggagatc atcctgttcg acgagccgga ctccgggctg gaccccgtgc gcaccgccta
     3961 cctgaaccag ctgatcatcg acctgaatgc gcagaccgac tccacgttcc tgatcgtcac
     4021 ccacgacatc aacacggcgc agaccgtgcc ggacaacatc gggatgctgt accgcaagca
     4081 cctggcgatg ttcgggccgc gcgaggtgct gctcacgtcc gaggagccgg tggtcgcgca
     4141 gttcctgaac gggcgcaggc agggcccgat cggcatgtcg gaggagaagg acaccgccca
     4201 ggcggccagg gagatggagg aggccgggga gctggccggg ctgccgccga tgaagccgca
     4261 gctgcagccg agcccgggcg tgggggagcg caaggccgtc ggccgtcgcc gcgagcgggt
     4321 gcagcagatg ctccacacgc tgcccgccgc cgctcagcag gcgatccggg cgagctacgc
     4381 cgacgatccg ctgcccgccc cgcggcctgt cggcccaccg gcccgaccgg cgtcctcgtg
     4441 gaggatgggc aggcggtcgc ctgcgcgaac ggacgactcg gtgcccggct acccgccgcg
     4501 ggcgccgggg tacggccccg aggccgacag cttcgatacg ttcgaccagg gctacggcgg
     4561 gggtgacccc gcgggtggcc cgggatcggg accgggctct ccgcccccgc catccggtcc
     4621 cggccggcat cacatctgag gcggcgacga tgacctcacc tgccccgctc acgcgtgcgc
     4681 tcacaccggt cggacggctc ttcggactcg gcgtcgccgt cgtccgcaac accttccggc
     4741 tgccgttcca gctcagcgag tacatcgagc agacctggtt cgtcaccaag gtctccgcgc
     4801 ttccgaccgc cctgttcacc atccccttcg gcgccacgat cgcgctgctc ctggccgacc
     4861 tcacccagca gttcggcgcg cagagtcaga ccggcgccgg cagcgtgctc gcgatcgtcc
     4921 agcaggccgc gccgatcgtc acggcgctgc tgatctccgg tgccggtggc agcgctgtgt
     4981 gtgccgacct cggtgcgcgc acgatccgcg aggagatcgc ggccatggag gtgctgggca
     5041 tctcgccgat ccagcggctc gtcgtcccgc gggtgctcgc gatggcgacg acggcggtgg
     5101 tgctcaacgg gctggccacg gtggtgggcg tcgccggtgg ctacttcttc aacgtcgtgg
     5161 tgcagggcgg cacgccgggc gcgtacatcg cgagcttctc cgcgatcgcg caggtctccg
     5221 acatcctcgt gagcgagatc aaggccgcct tgttcggctt caccgccggt gtcgtggccg
     5281 cctaccgcgg gctcaaccca ccgccagggc ccaagggcgt gggcgacgcg gtgaaccagg
     5341 ccgtcgtcat ctcgttcgtg ctggtgttcc tgatcaacct ggtgctgacg tcgctgtacc
     5401 tcgagttcgt gccggcgaag gggtcgtgac gtggcgctgc tgaacggcag ggcgcgccag
     5461 gtggtgaacg cgccgctgca ggtgctggag gagctgggcg agcagctctc gctctacgcc
     5521 cgcgcgatcg tgtggatccc gcgcacgctg cgccgctacc gctcggagat cgcccggctg
     5581 ctggccgagg tgagcttcgg ctcgggcgcg ctgatcgtca tcctcgggac ggcgggcgtg
     5641 atgctgtcgc tgtcgctgtt cgtcggcagc ctcgtgggcc tgcagggctt ccgggcgctg
     5701 gactcgctcg gggtggatgc gctcaccggt ttcatcaccg cgtacttcaa cacccgtgac
     5761 atcgcgccgc tcgtggcgtc ggcggcgctc accgcaaccc tcggcgccgg gttcaccgcc
     5821 cagctcggcg cgatgcggat ctcggaggag attgacgcgc tggaggtgat ggcggtgccc
     5881 agcgtgccgt tcctgatcac cacccgcgtg atcgccgggg tgatcgcgat catcccgatg
     5941 tacacgatcg ggctcctggc gagcttcgcc gcttcgcggc tcaacgtcac gctgatcaac
     6001 aacctccccg gtggcacgta cgaccactac ttcaacttgt tcctcccggt cagcgacgtg
     6061 ctgtactcgt atctcaaggt gatcatcttc gcgatggtga tcatcctgat ccactgccat
     6121 tacggctaca acgccaaggg cgggcccgct ggggtcggcg tggcggtggg ccggtcggtg
     6181 cggctgtcga tcgtcgccgc cgcggtgctg gacttcttcc tcacgctcgt gatctacggg
     6241 acggacacgt ccgtgcgggt ggcgggatga aggccgtccg tgggctcgcg ttcctcggcg
     6301 tgctcgtgct cctggccggg ctcgccgtcg cgcagtacgc gggcgcgttc cgctcaggcg
     6361 tgcccgtcac gctcaaggtc gcccgggtcg gcacccagct cgacccgaag gccgacgtca
     6421 aggtgcgcgg gctgatcgtg ggcagcgtga cgtcgatcag taccgacggc gcgggcgcca
     6481 ccgtcgcgct gtcgctcgac ccgcgcatgg tgaacgagat cccggccgac gtc
//
LOCUS       DASRSC010000139        23268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5121722,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000139 DASRSC010000000
VERSION     DASRSC010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 23268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23268
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5121722"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      complement(5..112)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            194..634
                     /locus_tag="VFE39_06630"
     CDS             194..634
                     /locus_tag="VFE39_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET6258310.1"
                     /translation="MPLDGEYELSPEGWVRDQTEKILETGTTTAVQIMDRPVILLTTR
                     GAKSGKLRKVPLMRVEHDGTYAIVASLGGAPKHPVWYHNVKSNPQVELQDDTVTKDYT
                     AREVTGDEKAQWWERAVAAYPEYENYQKKTDRQIPVFVLEPVEA"
     gene            complement(725..850)
                     /locus_tag="VFE39_06635"
     CDS             complement(725..850)
                     /locus_tag="VFE39_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258311.1"
                     /translation="MTDMIDAVLGTAAWMAGVLLLVVMAALPLLEHLAVPRPTRR"
     gene            969..2147
                     /locus_tag="VFE39_06640"
     CDS             969..2147
                     /locus_tag="VFE39_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6258312.1"
                     /translation="MIRLLRWVRRNADLVVELAAYGVWFVPDMVVAANSGTYVPVLAG
                     IVVPGIVLLRRRPGTDLVTVSAFAVGTSLAISVLTATPVVALLGANPRPPFFLSFTEL
                     LALAVIVVAVLRRCELRPALVLTVAAAVAIVGSPVIRHVTGDALGLALPCALGWGAAV
                     AVGLVLREVETRRRAALADVRSAERMELARELHDVVAHHVTGIVVAAQAAAVVARRSP
                     DDVDRALAAIETAGSDALAAMRRMVGVLRGQDAEATRTPGAELREVRTLVDRFDPESR
                     LVRLATAPGLEHAVLPAGIAATGYRVVQEALTNVRRHAPDATSVEVDVRMREEALLVS
                     VRNDGVPAAGAGPRAGSGGFGLAGMAERVGALDGTLTAGPTAPGVWTVSVRLPLRGSR
                     "
     gene            2144..2782
                     /locus_tag="VFE39_06645"
     CDS             2144..2782
                     /locus_tag="VFE39_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019812699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6258313.1"
                     /translation="MIRVLLADDQEMVRTGFRLILSAEDDVEVVGEAGTGVDAVARAR
                     TLRPDVVLMDIRMPELDGLEATRVLMADREPPRIVVVTTFDLDEYVYGALRVGACGFL
                     LKDAGPRLLVEAVRAAAAGDALVSPSVTVRLLKHLAARPATRAALPDPLSPRELDVVR
                     AVARGRTNAEIAAELFVSLSTVKTHLTNVQAKLSARNRVEIAAWAWEHGHVH"
     gene            complement(2848..3750)
                     /locus_tag="VFE39_06650"
                     /pseudo
     CDS             complement(2848..3750)
                     /locus_tag="VFE39_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
     gene            complement(3823..4395)
                     /locus_tag="VFE39_06655"
     CDS             complement(3823..4395)
                     /locus_tag="VFE39_06655"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET6258314.1"
                     /translation="MAVRPIRIIGDPVLHTPTRRVEVFDDALKALVEDMFDTMEAAEG
                     VGLAANQIGVDLRLFVYDCPDEELRARVRGVVVNPVLETSERPETMPDPEDDEEGCLS
                     VPAEHFPTGRAEWARVTGVDVDGAPVQVEGRGFLARCLQHETDHLDGVIYLDRLIGRH
                     QRAARKMLRHNGWGVPGLAWDPATEQAEDV"
     gene            4552..4857
                     /locus_tag="VFE39_06660"
     CDS             4552..4857
                     /locus_tag="VFE39_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HET6258315.1"
                     /translation="MESATGPNGRSPAGSAHRELDRREREILAFEGQWWKYAGAKEQA
                     IRELFDMSATRYYQVLNALIDKPEALAADPLLVRRLRRLRSSRQRTRAARRLGIEPW"
     gene            4914..5600
                     /locus_tag="VFE39_06665"
     CDS             4914..5600
                     /locus_tag="VFE39_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HET6258316.1"
                     /translation="MTAPASSGGPSPLRIGGIALLGIGVIAGIVGLAMLATNGGGNGG
                     QTAAPPPSTQATDTAGPSPSAFVPPPPTDTTPTGSVPVPVPSFGPTPTNGIAGPSGTG
                     SGAPPQSPNGGAGSKDGGAGSGGAGSGTLRAPVRVYNNSTITGLAARAAADFRSAGWQ
                     VEEVGNYPYGIIPTSTVYYRPGTGEQQAAQAIGDQFSLKVEPRFEGLDEASPGLIVIA
                     TNDYRGRSRR"
     gene            complement(5619..7901)
                     /locus_tag="VFE39_06670"
     CDS             complement(5619..7901)
                     /locus_tag="VFE39_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6258317.1"
                     /translation="MSAPSITLVARLAASAADARRGVVRLHPEVIDALGLRSWEAVTL
                     TGARVTTALVAPAGGPAGQATLDDVTLSNAGLSDGAAVVVAPAVVEPARRVLLTGSRL
                     ARAALTPEIVRLALLGKVVTGGDAVSLLPQDVEPAPGLDAPTARRRVAGALGSSWTTE
                     LLTVATVEPAGAVAVLPSTVVGWEGGAATGAPVLAAGPSAAPAPDRRVAQGRPASTTA
                     GAPAPSVVAVAAAAEPAVAVEDLVGNTDAARRLVEWLELTLDRPELLTRLGGSARLGV
                     LVVGPEGVGKLTLARSTAAAVGASCVELAAPGVAALEAGTASQRVHEVVGSARAAARA
                     GSRTVLVVTDVDALLPAASPPPLSTIVLDALREAVGTPGLALVATTSSPESVDPRLRA
                     PDLMDRELPLALPDLRTRIELLRRLLAAVPLADGVDLKAVAERAPGFVAADLVAARRE
                     AAVQAALRHSGDGEPRICQQDLLDAVGSVRPISMSSSDTLQTGGITLDQVGDMVEVKQ
                     ALTEAVLWPLQYPDSFARLGVAAPRGVLVYGPPGGGKTFLLRALAGTGQLNVLAVKGA
                     ELLDKYVGESERAVRELFRKAADAAPALVFLDEVDALAPRRGQSADSGVADRVVAALL
                     TELDGAEPLRDVIVVGATNRPELIDPALLRPGRLERLIYVPPPDAGARAEILRASGRN
                     IPLADDVDLDALGAELEGYSAADCAAVLREAALTAMRESLDAAVVTGAHIAAARAAVP
                     PSLEAEQVAALQAYADRRPS"
     gene            complement(7990..9021)
                     /locus_tag="VFE39_06675"
     CDS             complement(7990..9021)
                     /locus_tag="VFE39_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HET6258318.1"
                     /translation="MQPPPAYAAGIRLLPNAVTVLALCAGLSAVYFALSGRFELCVAA
                     AGAAALCDALDGRLARLLDASTRIGAELDSLADLVSFGVAPALVMYIWALEGSRFGWI
                     VALVFAVCMALRLARFNTLIDAEDEYPFAKEFFVGVPAPAAALSAGLPLYLTLHFGPG
                     WWSAPVLVGLWALVVAGLMVSRLPTLSFKTVRVPPNYIAPLLVLVGIAAAVLITAPFL
                     GLAIVAVVYLALIPYTGYRYRWLARHPEAWGIPVRERRAVARAARSARRLGLRSPLRR
                     RVAGRASAVARAVRRFGDDPTGSMRRFGDDPSGSVQRGPTANGRAPGPAPLPPRGRLR
                     LGLRRARRR"
     gene            complement(9021..9773)
                     /locus_tag="VFE39_06680"
     CDS             complement(9021..9773)
                     /locus_tag="VFE39_06680"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HET6258319.1"
                     /translation="MPDPNGAPVGPLPSEPSHAGLSLPHVAGLIRAAVPPMHPGGRPL
                     VAAVAATAAVIRTVTGRGAVAGALATAATAAFFRNPRRVRPPLAGVVLSAADGTVATI
                     SEVLPPPELDLPRRPVSRVSVFLSVLDVHVQRIPADGRVLAVEYRPGTFLSADLDKAS
                     EDNERNALLMETTDGAQLGVVQIAGLLARRIVCDVRPGDLVAAGDTYGLIRFGSRVDV
                     YLPPGSRVTVAVGQRTIGGETVLASLPGCEAF"
     gene            complement(10174..11400)
                     /gene="glp"
                     /locus_tag="VFE39_06685"
     CDS             complement(10174..11400)
                     /gene="glp"
                     /locus_tag="VFE39_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET6258320.1"
                     /translation="MASFQRTVEEHRAVVAALLPPMPAEDVPIRQARGRVLAADVVAA
                     VSLPSFDNSAMDGYAVRAADLAGGGAASPVELPVSADIPAGRLDVPPLEPGTAHRIMT
                     GAALPPGADAVVPVEDTDGGIGRVRLTAAPAPGAYVRYSGEDVRAGDVVLAAGTVLGP
                     PQIGVAAAVGSVTVAVRRRPIVLVLSTGSELVAQGTALQPGQIYESNGPMLAAAVEDA
                     GGHAELLRFVPDDVEQFLDRLRRRLDAGRVDLVLTSGGVSAGAYEVVKDAFTGRGVEF
                     VKVAMQPGGPQGAGSLAELGGVGVVTLPGNPVSSHVSFEVFVRPALRASFGHPYPDRP
                     VVTATLSERWTSPLGRRQFRRGVLDAVHGTVREVGTPASHLLGALARAECLVVVPEDV
                     TELDPGALVQVLLLEP"
     gene            11505..11708
                     /locus_tag="VFE39_06690"
     CDS             11505..11708
                     /locus_tag="VFE39_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HET6258321.1"
                     /translation="MAQGTVKWFNAEKGFGFIAMDGGPDVFVHYSAIQSDGFRTLDEG
                     DRVEFETTAGRDGRSQADAVRRI"
     gene            11983..13611
                     /gene="groL"
                     /locus_tag="VFE39_06695"
     CDS             11983..13611
                     /gene="groL"
                     /locus_tag="VFE39_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631882.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET6258322.1"
                     /translation="MAKMIAFDEEARRGLERGMNTLADAVKVTLGPRGRNVVLEKKWG
                     APTITNDGVSIAKEIELEDPWEKIGAELVKEVAKKTDDIAGDGTTTATVLAQALVREG
                     LRNVAAGANPMALKRGIEKAVEAVSQQLLKTAKEVETKEQIAATASISAADSTIGELI
                     AEAMDKVGKEGVITVEESQTFGLELELTEGMRFDKGYISPYFVTDAERMEAELEDPYI
                     LLVSSKISTVKDLLPLLEKVMQTGKPLAIIAEDVEGEALATLVVNKIRGTFKSVAVKA
                     PGFGDRREAMLADMAILAGGEVISEKVGLKLENADLSLLGRARKVVVTKDETTIVDGA
                     GDADQIAGRVNQIRSEIERTDSDYDREKLQERLAKLAGGVAVIKAGAATEVELKERKH
                     RIEDAVRNAKAAVEEGIVAGGGVALIQAGAGLFEGLGLDGDEATGANIVKVALEAPLK
                     QIAINAGLEGGVVAEKVRGLNAGEGLDAATGEYKDLIKAGIIDPAKVTRSALQNAASI
                     AALFLTTEAVIADKPERNPAPAGDPSGGMGGMDF"
     gene            complement(13758..14729)
                     /locus_tag="VFE39_06700"
     CDS             complement(13758..14729)
                     /locus_tag="VFE39_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET6258323.1"
                     /translation="MRADRLLSLLLLLQHRGRMSATALAGELEVSTRTVLRDVEALSA
                     AGVPVYAERGRHGGFALLPGYTTDLTGLTHDEALALLVAGSQAAAPSPGTAPALASAM
                     RKVVAALPQQQRTAATRDAGRILVRREGMVRAGGGPAAPDDEERLAVVRRAVFAGRKL
                     RIHYAASGEETRWRTVDPVGLVSARGQWYLLATRDGADRTYRLSRLLDVEELAEPADR
                     PAGVDLQRTWEERRARFAATLPGLTATVRVRPDVRASLVRTVREVAAERDDGDGWLRL
                     DLEFAALDHALSVVWALGPDAEAIGPPELRAVLVERATATAARYVAG"
     gene            14793..15392
                     /locus_tag="VFE39_06705"
     CDS             14793..15392
                     /locus_tag="VFE39_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HET6258324.1"
                     /translation="MTTTEITDPRPLYAAALSWAHDVVRGVRPSQLDGPTPCTEFDVR
                     TLLAHLVATVDRAAALGKGIDAASTPSFVPAAPDDGWGTAYGDAISAMWTVWRDGPGS
                     DDLLDRTVTAPFGTVPGRAAVLAYTSETLVHGWDLAVATGQDAEANPAVARAVLAMMQ
                     RFLPAEPRGGRIPFAPPVPPRDDAGPTEQLANWCGRGRA"
     gene            15801..16838
                     /locus_tag="VFE39_06710"
     CDS             15801..16838
                     /locus_tag="VFE39_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6258325.1"
                     /translation="MNERVATASVTLLDVARAAGVSLATASRALNGSTRRVRPDLRDR
                     VLEAADALQYSANAQAQAMARGHTNLVGLVISDVGDPYFSSIAAGAIRAAEDHQLVVT
                     MACTFGREEREIDYVAALRSQRARAVIVVGSRVHDRGLTDRLGAEVRSFEQSGGRVAF
                     ISQRKIAADTIVLENRAGARALAEALCCRGYRRFAILAGRADLLTARDRSAGFREGAA
                     RHGVQIAQDDVVHGGFNRDGGYDAMIEVLDRGRDVDCVFAVNDVMALGAMAALRDRGV
                     ALPSGMAVAGFDDIVTLRDVTPALTTVRLPLEDLGAWALQLVLEPSTDQPRIRRVRGE
                     VVLRESTPVRP"
     gene            17154..18032
                     /locus_tag="VFE39_06715"
     CDS             17154..18032
                     /locus_tag="VFE39_06715"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6258326.1"
                     /translation="MTTTKAVTVIGTGIMGAGMARSLARAGLTVTVWNRDAGKARPLA
                     DVGATVADDPASAVAGADVILTMLFDVESVEQVMGQALPAAAEGAVWVQSSTVGLDGA
                     LRLAELADRAGIDYVDAPVLGTRQPAENGTLLVLAGGPERLRDTVAPVLDAIGSRTVW
                     VGPRPGDGHRLKLVANSWVLSVVGGTAQAIGLAEGLGLDPQLFLDTIAGGATDSVYAQ
                     VKGSAMIAGDFPPSFTLDGAVKDAGLIADALRGAGVDDRLARALHQQFASAAEAGHGA
                     EDLAAVVEAVRNPPSS"
     gene            complement(18073..19734)
                     /locus_tag="VFE39_06720"
     CDS             complement(18073..19734)
                     /locus_tag="VFE39_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair helicase XPB"
                     /protein_id="HET6258327.1"
                     /translation="MTDGPLIVQSDKTLLLEVDHPAAAEARAAIAPFAELERAPEHVH
                     TYRVTPLALWNARAAGHDAEQVVDALVRFSRYAVPQPLLVDVVDTMGRYGRLQVTNSP
                     VHGLVMVALDRAVLEEVLRQKKIAPLLGTRVDDDTVLVHPSERGHLKQALLKIGWPAE
                     DLAGYVDGEAHPIALAEDGWSLRDYQRQAVDGFWAGGSGVVVLPCGAGKTLVGAAAMA
                     EAQATTLILVTNTVAGRQWKRELVARTSLTEEEIGEYSGERKEIRPVTIATYQVVTRK
                     TKGEYKHLELFDSRDWGLVIYDEVHLLPAPVFRMTADLQSRRRLGLTATLVREDGREG
                     DVFSLIGPKRYDAPWRDIEQQGWIAPADCVEVRVTLTDAERLGYAVAEAEERYKMAST
                     AHTKLGVVKAILGKHPGEPALVIGAYLDQLEELGEALDAPIIQGSTKNREREALYDQF
                     RRGELPVLVVSKVANFSIDLPEASVAIQVSGTFGSRQEEAQRLGRLLRPKGDGRQAHF
                     YSVVSRDTLDTDYAAHRQRFLAEQGYAYRIVDADDLLGPAIPDVG"
     gene            20191..>23268
                     /locus_tag="VFE39_06725"
     CDS             20191..>23268
                     /locus_tag="VFE39_06725"
                     /inference="COORDINATES: protein motif:HMM:NF019974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate- and nitrite sensing domain-containing
                     protein"
                     /protein_id="HET6258328.1"
                     /translation="MTSIETGPTWSERFNPRNWTLAWKLVIVCLIPALLALALGVLRV
                     SDQAGQAADLGRSTRLLEVAQRVSGAADTLRQERNASTIFAAGRRTDDRNALEAGFRQ
                     TDGVLDETLGAVHSTTGLEVTTQSALQKAERELATLGPLRTDAAQNPAVSSDVISSRY
                     TTVIDTVDVLGRALLRQLSTPGIGGLPDALTAVVNISEQLAIQHTVLSAAIRSGSLRP
                     TDVSAVNSTDSQLDAAAREYQTALTAGVVGNPLDATANTQRELLKSAILATPPARPIG
                     VTLAAWDAAHADAQAAADRASTRIRKALSSASAAAEERASNEAGLNSVVLMLGLLIGA
                     TIAVLVSRALLRSLQVLRTTALDVAERRLPQAVEGMRAGETPDVEVDPVPLSGRDEVA
                     QVARAFDAVHGQAVRLAAEQAALQSNVSAMFVNLSRRSQALVERQLQLIEQLETNEQD
                     PDQLSNLFQLDHLATRMRRNSENLLVLAGTDLAKRNIAPIPMVDVLRAAVSEVEQYQR
                     IVVQTPPTVTVVGRAASDLVHLLAELLDNATSFSPPDSQVVMSTARTADGSIVVEIAD
                     RGVGMIDHELSDANQRLGGPSSVDVSASRRMGLFVVGRLGARHGISVRLSTSSAGGPG
                     AGLTASVTVPAYLIPSSEPAETRRQPGALPVRGNALLPPRAGTNGNARPGSLSALVAG
                     TDGPANPAGIFDAPAGKDGQLFAPQPRPTMNGSSGKLPTRRPGSTVRPEGASSTRPDP
                     AEQAAADQAAAEQAQEKAREVAAQAAADAELAEQQQRDQAQRSAAQGQQAGAHATGFY
                     RNGAQPDTARSLWSTGGDKSSANGFVPNDAPAANGSTPHGAVTNGSAPEGTQSDDGPT
                     PRQQPAASSEAESTSGAEAAADQSGSGQDRPDERSPDAQQTGTFPAETAFGNGAQTQR
                     GAPTQVPPSPPTMPMPVVGRGASMPPPRPAVQRPERPAQQQAAPRPFAQSPGSAEHTQ
                     QQPVQHAQPAQPAESAQPAQRAQPVQPAESAQPAQHAQPVQPAESAQPAQHAQP"
BASE COUNT         3443 a         8613 c         8229 g         2983 t
ORIGIN      
        1 gcgcgttcgc tcatgcttcc ctcccgacgc cggcattacc cggctggttc gacggtcggc
       61 ggcgcccagc cgccctctca gcccgctggc gcgagctccc gtgtgctcat gaccgtaacc
      121 cgagctgcgg ctgatgcgcc agaacaccgg ccctccggga gcaaacgacg gcatggaggt
      181 gttatctagc gacatgccac ttgacggtga gtacgagctg agtccagaag gctgggtccg
      241 ggaccagacc gagaagatcc tcgaaaccgg gacgacaaca gctgtccaga tcatggaccg
      301 gcccgtgatc ctgctgacga cccgcggcgc gaagagcgga aagctgcgca aggttccgct
      361 catgcgcgtg gagcacgacg gcacctacgc catcgtcgcg tccctcggcg gcgccccgaa
      421 gcacccggtg tggtaccaca acgtgaagtc caacccgcag gtcgaactgc aggacgacac
      481 ggtgaccaag gactacaccg ctcgtgaggt caccggcgac gagaaggccc agtggtggga
      541 gcgggccgtt gccgcctacc cggagtacga gaactaccag aagaagaccg accggcagat
      601 tcccgtcttc gtgctggagc cggtcgaggc ctgagccgcg gtacacggcg gcggcagcgg
      661 ctcggcgggg gcggccggtt ccgagggtgc cgcgctgctc gggagaccgg taccagcggc
      721 gcggtcatcg ccgagtgggg cgcggtacgg cgaggtgctc cagcagcggc agggcggcca
      781 tcacgacgag gagcaggacc cctgccatcc aggccgccgt gcccaacacc gcatcgatca
      841 tgtccgtcat ggctgtcacg atctcccgca gctgcggtgc gtcgcctcgt ccggacgacc
      901 gaaatcgccc ggccgagctc gtacttccgg ccgagggagc gggagcgcgg gcgcaatagg
      961 ctcggtgcat gatccgcctg ctccgctggg tccggcggaa cgccgacctc gtggtcgagc
     1021 tggccgccta cggcgtgtgg ttcgtgcccg acatggtcgt cgcggccaac tcgggcacgt
     1081 acgtacccgt cctcgccggc atcgtggttc ccgggatcgt gctgctgcgc aggcggccgg
     1141 gcacggacct ggtcaccgtc tcggcgttcg ccgtgggcac ctcgctggcg atctcggttc
     1201 tcaccgcgac ccccgtggtg gcgcttctgg gcgccaatcc ccggcccccg ttcttcctct
     1261 ccttcacgga gctgctcgcg ctcgccgtca tcgtcgtggc cgtgctgcgc cggtgcgagc
     1321 tgcgcccggc gctcgtgctc accgtggccg ccgcggtggc gatcgtcggg tcccccgtca
     1381 tccgccacgt caccggcgac gccctgggac tcgccctgcc ctgcgcgctc ggctggggcg
     1441 ctgccgtcgc cgtcgggctg gtcctgcgtg aggtcgagac gcgccggcgc gccgcactcg
     1501 cggacgtccg gtcggcggag cggatggagc tcgcccgcga gctgcacgac gtcgtcgccc
     1561 accacgtcac cggcatcgtc gtggccgctc aggccgcagc ggtggtggcc cgccggtccc
     1621 ccgacgacgt cgaccgggcc ctcgccgcca tcgagacagc cggttcggac gcgctggcgg
     1681 cgatgcgccg gatggttggg gtgctacgcg gtcaggacgc cgaggccacg cgcaccccgg
     1741 gtgcggagct gcgcgaggtc cggacgctcg tcgaccggtt cgaccccgag tcccggctcg
     1801 tccggctcgc gaccgcaccc gggctggagc acgccgtgct gccggctggg atcgccgcca
     1861 ccgggtaccg ggtcgtgcag gaagctctga ccaacgtccg caggcacgct ccggacgcca
     1921 cgtccgtcga ggtggacgtc cggatgcgcg aggaggctct gctggtgtcg gtgcggaacg
     1981 acggggtgcc cgcggccggg gcggggccac gcgccgggtc gggcgggttc gggctcgccg
     2041 ggatggccga gcgggtcggc gcgctggacg gcacgctcac cgcgggcccg acggcacccg
     2101 gcgtgtggac ggtctcggtc cggctgccgc tgcggggctc gcggtgatcc gggtcctgct
     2161 cgccgacgac caggagatgg tgcgcaccgg attccggctg atcctctcgg cggaagacga
     2221 tgtcgaggtg gtcggcgagg ccggcaccgg ggtggacgcc gtcgcgcgcg cccgcacgct
     2281 gcgcccggac gtggtgctca tggacatccg catgccggag ctggacgggc tcgaggccac
     2341 gcgcgtgctg atggccgacc gcgagccgcc gcggatcgtc gtggtcacga cgttcgacct
     2401 cgacgagtac gtgtacggcg cgctacgcgt cggagcatgc gggttcctgc tcaaggacgc
     2461 ggggccgcgg ctgctcgtcg aagcggtgcg cgccgcggcg gcgggggacg cgctcgtgtc
     2521 cccgtcggtc accgtgcggc tgctgaagca cctcgccgcc cggccggcca cgcgggcggc
     2581 gctgcccgat ccgctgtccc cacgggagct ggacgtggtg cgcgccgtcg cccgtgggcg
     2641 tacaaacgcg gagatcgcgg ccgagctgtt cgtctcgctc tcgacggtca agacgcacct
     2701 gaccaacgtg caggccaagc tgtcagcccg gaaccgcgtg gagatcgccg cgtgggcgtg
     2761 ggagcacggc catgtgcact gatcagaggt cgtgcgcgga tacgagtgct ctgacactcg
     2821 tatccgcgcc cgggggcggc agccccgttc ggtggggacc gcgaccacgt ccaccatcgc
     2881 gccgttgcgc agcaccgtga cgggcagcgg caccccgatc gcgtccccga agagctgccg
     2941 ttgcacgccc tgggcgtcct gcaccgggcg gcggccgacg tcgagcacga ggtcgcccgc
     3001 ccggaggccg gcccgcgctg ccgggctgcc gccgatcacc tcggcgagcc gcagcccgct
     3061 gctgcgcccg tagcgctcgg cgatcttcgc gggcacaggt gccggactcc cgacgacacc
     3121 gaggtacgcg cggcgcacgc gcccgtccgc gaggagtgcg gccacgatcc gccgagtcgt
     3181 cgcgttcacc agcaccgcca gcccgagtcc gacacccgcg acggcggtgt tgatcccgac
     3241 cacgcgcgca tggctgtcgg cgagcgctcc ccccgagttg ccggggttga gcgccgcatc
     3301 ggtctggatg acgtcctcca cgacgcggcc cgcgcttccg ctgcgggtgg gcagggcccg
     3361 tcccagcgcg ctcacgactc ctgctgtcac ggagcctgcg aggccgagcg ggttgcccac
     3421 ggccaccacg agctggccga ccacgaggtc gtcggcgttg ccgagccgca ccggcggcgg
     3481 caggtcgccc ggacgtgcgg cgcggagcac ggcgaggtct gagagcgggt cggtacccac
     3541 gacagtgacg tcggcctggg tgccgtcgac gaagtcggcc cggccccggg aggcgtcgcc
     3601 gaccacgtgg gcattggtga ccagcacgcc ggcggcgtcg acaaccaccg ccgagccgct
     3661 gccgcgcccg gtgcggatcg ccgccacccg gccggtgagc tcggcggcca cagcgctgac
     3721 cgtgcgggag taggagtcga gctcgtccac gacggcctcc tgctgcttac aatgttgcag
     3781 aggtgttgca gcagaagaac gcagcgccgt gcgatggtgt tcctacacgt cctcggcctg
     3841 ctcggtcgcg gggtcccagg cgagcccggg cacgccccag ccgttgtgcc tgagcatctt
     3901 gcgcgcggct cgctggtggc ggccgatgag ccggtcgagg tagatcaccc cgtcgaggtg
     3961 gtcggtctcg tgctggaggc acctggccag aaacccacgc ccctccacct gcaccggcgc
     4021 cccgtcgacg tccactcccg tgacgcgcgc ccactccgcc cggcccgtcg ggaaatgctc
     4081 ggcaggcacc gaaaggcagc cctcctcgtc gtcctccggg tcaggcatcg tctcgggacg
     4141 ctccgaggtc tccagcaccg ggttgacgac gacgccacgc accctggcgc gcagctcctc
     4201 gtccgggcag tcgtagacga acaagcgcag gtcgaccccg atctggttcg ccgcgaggcc
     4261 gacgccctca gctgcctcca tcgtgtcgaa catgtcctcg acgagggcct tcaacgcgtc
     4321 gtcgaacacc tcgacgcggc gggtcggcgt gtgcaggacg gggtccccga tgatgcggat
     4381 gggtcggacg gccacggacg acgagcctag tcgtccggtc acctcaggcc ctcgatccac
     4441 tccaccgagt ggtgcgtgcc taacccgttc tcgggggtgc cccgtggttg aatggcggcc
     4501 gcgaatgaca ggtgtgtcat tcatcgaact gcgaggccag cgaggagcgt gatggagtcc
     4561 gccaccggac ccaacgggag gtctcccgcc ggaagcgcac accgcgagct ggaccgcagg
     4621 gagcgcgaga tcctcgcttt cgagggccag tggtggaagt acgccggcgc caaggagcag
     4681 gcgatccgcg agctgttcga catgtccgcc acccgctact accaggtgct caacgcgctc
     4741 atcgacaagc cggaagcgct cgccgccgac ccgctgctcg tccggaggct ccggcggttg
     4801 cgctccagcc ggcagcgcac ccgcgcggcg cgtcgcctgg gcatcgagcc gtggtgacgg
     4861 caccatgatc accacaggtc gctgatcgaa atccatccca ctcggaggca ttcgtgaccg
     4921 cacccgcttc gtccgggggc ccgtctccgc tgcggatcgg cggaatcgct ctgctcggga
     4981 tcggggtcat cgcgggcatt gttggcctcg caatgctcgc caccaacggt ggcggcaacg
     5041 gcggccagac ggctgcgccg ccgccgtcca cccaggcgac cgacaccgcg ggccccagcc
     5101 cgagcgcctt cgtgccgcca ccccccaccg acacgacccc caccggctcg gtgccggtgc
     5161 cggtgccgtc cttcggtccc acccccacca acggcatcgc cgggccgtcc ggtaccgggt
     5221 cgggcgctcc tcctcagtct cccaacggag gcgcaggcag caaagacggc ggcgcgggat
     5281 cgggcggcgc gggcagcggc accctccggg ctccggtacg cgtctacaac aacagcacca
     5341 tcaccggcct cgcggcacgc gccgccgccg acttccgcag cgccgggtgg caggtcgagg
     5401 aggtcggcaa ctacccgtac gggatcatcc cgaccagcac tgtctactac cggcccggca
     5461 cgggcgagca gcaggcggcg caagccatcg gcgaccagtt ctcgctcaaa gtcgagccgc
     5521 ggttcgaggg cctggacgag gcgagccctg gcctgatcgt catcgccacc aacgactacc
     5581 gggggcgctc acgccgctga gcctcggggc ctcccaggtc aggacgggcg gcggtcggcg
     5641 taggcctgca gtgccgcgac ctgctcagcc tccaatgacg gcgggacggc ggcccgggca
     5701 gcggcgatgt gcgcccccgt caccactgcg gcgtcgagcg actcccgcat cgcggtgagc
     5761 gcggcctcgc gcagcacggc ggcgcagtcg gcagccgagt agccctcgag ctccgcaccg
     5821 agggcatcaa ggtcgacgtc gtcggccaga gggatgttcc gtccggacgc gcgcaggatc
     5881 tcggcgcgcg cgccggcgtc cggcggcggg acgtagatca accgctccag ccgcccaggg
     5941 cgcagcagtg ccgggtcgat cagctcgggc cggttcgtgg cgcccacgac gatcacatcg
     6001 cgcagcggct ccgcgccgtc cagctccgtg aggagcgcgg cgacgacacg gtcggccaca
     6061 cccgagtcgg ccgactgccc gcggcgcggg gccagcgcat cgacctcgtc caggaacacg
     6121 agggccggcg cggcgtccgc ggccttgcgg aacagctcgc ggaccgcccg ctcggactcg
     6181 ccgacgtact tgtcgagcag ctccgcaccc ttgaccgcga gcacgttgag ctgcccggta
     6241 ccggccagtg cgcgcagcag gaaggtcttg ccgccgccgg gcggaccgta gaccagcaca
     6301 ccgcgcggag cggcaacgcc gaggcgggcg aatgagtcgg ggtactgcag cggccagagc
     6361 accgcctcgg tgagcgcctg cttgacctcg accatgtcgc ccacctggtc gagcgtgatc
     6421 ccgccggtct gcagcgtgtc cgacgacgac atcgagatcg gccgcacgga gccgacggcg
     6481 tcgagcaggt cctgctggca gatccgcggc tcgccgtcac cgctgtgccg gagtgcggcc
     6541 tgcacggcgg cctcacgccg cgccgcgacg aggtcggcgg ccacgaatcc gggggcgcgc
     6601 tccgctacag ccttgaggtc caccccgtcg gccagcggca ccgcggccag caacctgcgc
     6661 agcagctcga tgcgggtgcg caggtcgggc agcgccagtg ggagctcgcg gtccatgagg
     6721 tcgggagcgc gcagtcgcgg gtcgaccgac tccggcgacg aggtcgtggc gacgagcgcg
     6781 agccccggcg tccccaccgc ctcacgcagc gcgtcgagca cgatggtgga gagcggcggc
     6841 ggtgaggcgg ccgggagcag ggcgtcgacg tcggtgacca ccagcacggt ccgcgagcct
     6901 gcgcgggccg cggcccgagc cgagccgacc acctcgtgca cccgctgcga cgctgtgccc
     6961 gcctcaagcg cggccactcc cggcgccgcg agctccacgc aggaagcgcc gacggccgcg
     7021 gccgtgctgc gcgccagcgt cagcttcccg acgccctccg gaccgacgac gagcacgccg
     7081 agccgcgcag agccgccgag gcgggtgagc agctccggtc ggtcgagggt cagctcgagc
     7141 cactcgacga gcctgcgcgc agcgtcggtg ttgccgacga gatcctcgac cgccaccgca
     7201 ggctcggcgg ccgcggccac cgccactacg gacggagccg gggcgccggc ggtcgtgctg
     7261 gcgggcctcc cctgcgccac ccgtctgtcc ggggccggcg ccgccgatgg ccctgcggcc
     7321 agaacgggag cgccggtcgc cgcaccaccc tcccagccca ccaccgtgct cggcagcacg
     7381 gccaccgctc ccgcaggttc gacggtggcc acggtgagca gctccgtggt ccacgagctg
     7441 cccagcgccc cggcaacgcg ccggcgggcg gtgggtgcgt ccaggcccgg cgcaggctcg
     7501 acgtcctgcg gcagcagcga gaccgcgtcg ccgccagtga cgaccttgcc cagcagcgcg
     7561 agccgcacga tctcgggcgt cagtgcggcg cgggcgagcc gggagccggt gagcagcacc
     7621 cgccgggcgg gctcgaccac cgcaggcgcc accaccacgg ccgctccgtc ggacagtccg
     7681 gcgttggaga gcgtgacgtc gtccagggtc gcctgtccgg caggcccccc ggcgggggcg
     7741 acgagcgcgg tcgtcacacg ggctccggtg agggtgaccg cctcccacga gcgcaggccg
     7801 agcgcgtcga tgacctcggg atgcaagcgg acgacgccgc gccgggcatc cgctgcggac
     7861 gcggcgagcc gggcgacgag agtgatcgag ggagcgctca cgcccagaac gctaacgagc
     7921 agcccgagcc gcgtccgccc tcccccaacc cgcgacatcg acgcacgtcg cgtatccgga
     7981 gggcaggtgt cagcggcggc gggcgcggcg cagacccagc cgcagcctgc cgcgcggcgg
     8041 cagcggggcc ggaccgggtg cgcgaccgtt cgccgtgggg ccgcgctgca cggagccgct
     8101 cgggtcgtcg ccgaaccggc gcatggaccc cgtcgggtcg tcgccgaacc ggcgcacggc
     8161 ccgggcgaca gcgctggcgc ggccggcgac gcgacggcgc agcggggagc gcagcccgag
     8221 tctgcgggcg gaacgggcgg ccctggcgac ggcacggcgc tcccgcacgg ggatgcccca
     8281 tgcttccggg tgccgggcga gccacctgta ccgatacccg gtgtagggga tcagggcgag
     8341 gtagaccacg gcgacgatcg cgagcccgag gaacggtgcg gtgatcagca ccgcggcagc
     8401 gatgccgacg agcaccagca gcggtgcgat gtagttgggc ggcacccgca cggtcttgaa
     8461 cgacagcgtc ggcagcctgc tcaccatcag cccggccacg accagcgccc acagccccac
     8521 cagcaccggg gccgaccacc agcccgggcc gaagtgcagg gtgaggtaga gcgggaggcc
     8581 cgcggacagc gcagcggcag gagccgggac gccgacgaag aactccttgg cgaacgggta
     8641 ctcgtcctcg gcgtcgatga gggtgttgaa ccgggccagc cgtagcgcca tgcacacggc
     8701 gaagaccagc gcgacgatcc agccgaaccg gcttccctcc agcgcccaga tgtacatcac
     8761 cagcgccggc gcgacgccga acgacaccag gtcggccagc gagtcgagct cggcgccgat
     8821 ccgcgtgctc gcatcgagca accgggccag cctgccgtcg agcgcatcgc acagggcggc
     8881 agcgcccgcc gcggcgacac agagctcgaa ccgcccgctc agcgcgaagt acacagcgga
     8941 gaggccggcg cagagcgcga ggaccgtgac agcgttcggc agcagtcgga tgcctgcggc
     9001 gtaggcgggg ggcggctgca tcagaacgcc tcgcatccgg gcagcgaggc cagcacggtc
     9061 tcaccgccga tcgtgcgctg cccgacggcg acggtgaccc gcgaacccgg cggcaggtag
     9121 acgtcaacgc gcgagccgaa ccggatcagc ccgtaggtat cgcccgccgc gaccagatcg
     9181 cctgggcgga cgtcgcacac gatgcgtcgc gccagcaggc cggcgatctg cacgactccc
     9241 agctgggcgc cgtcggtggt ctccatcagc agcgcattgc gctcgttgtc ctcgctggcc
     9301 ttgtcgagat cggcggacag gaacgtcccg ggccggtact cgacggcgag cacgcgtccg
     9361 tcggccggaa tccgctgcac gtgcacgtcc agcacggaca ggaacacgct gacgcgggac
     9421 accggccggc gcggcaggtc cagctccggc ggaggcagca cctcgctgat cgtggccacg
     9481 gtgccgtcag ctgcggagag caccacgccg gcgagcggcg ggcggacgcg gcgggggttg
     9541 cggaagaagg cggcggtggc ggcggtcgcg agcgcccccg ccacagcacc tcggccggtg
     9601 accgtcctga tgaccgccgc agtggccgcc acggccgcca ccagcggacg acctccggga
     9661 tgcatcggag gaacggcggc gcggatcagc ccggcaacgt gcggcagcga cagtcccgcg
     9721 tgggacggtt cggagggtaa gggcccgaca ggggccccgt tcgggtcggg catgggggcg
     9781 ggtcggctcc tccgggtagc gactcgggac gatcgcagcg gcggttacgg tacgcgaaag
     9841 cggatcgtca ccgtgtgcca cactgccgtc accggtccga ggaaccggaa gaacgcaaag
     9901 ggccggggcc gccatccccc gagacgacga ccccggccgt ggaaccgctc gtccgctccc
     9961 cagcgacggg cggtaggtcc gttgcaacac ttccccggtt tcccggggcg ccccccaagt
    10021 atccccgagc acccgatcga tcgtccaggt cactggccca ggaaccctgc gatgtgcacc
    10081 gatcggccga acgtttcatc tgtaagacgc tgcgagagcg cgaattgttg cctccccctg
    10141 ctgtgaccta cgtcacattt tgcgccgacg gccttacggc tccaacaaca agacctgcac
    10201 gagggccccg ggatcgagct ccgtcacgtc ctccggaacg acgaccagac actccgccct
    10261 ggccagcgca ccgaggaggt gcgacgccgg cgtcccgacc tcccggacgg ttccgtggac
    10321 cgcatcgagg accccccgtc gaaactgtcg tcggccgaga ggtgacgtcc atcgctcact
    10381 cagcgtggcc gtcacaaccg gccgatcggg gtacggatga ccgaacgagg cacggagcgc
    10441 gggccggacg aacacttcga aggacacgtg cgagctgacc ggattgccgg gcaacgtcac
    10501 caccccgacc ccgcccagct ctgcgaggct ccccgcgccc tgcggcccgc cgggctgcat
    10561 ggcgaccttg acgaactcca cgccccgacc ggtgaacgcg tccttcacca cctcataggc
    10621 tccggcgctc accccgcccg aggtgagcac gaggtcgacg cgcccggcgt cgaggcgccg
    10681 ccggagccga tcgaggaact gctcgacgtc gtcgggtacg aaccggagca gctccgcgtg
    10741 gccgcccgcg tcctcgaccg cggccgcgag catcggtccg ttcgactcgt agatctgccc
    10801 cggctgcaac gcggtgccct gcgccaccag ctccgagccc gtggagagca ccaacacgat
    10861 gggcctgcgg cgcaccgcca ccgtcaccga accgaccgcg gctgccacgc cgatctgcgg
    10921 cgggccgagc accgtgcccg ccgcgagcac gacgtcgccc gcccggacgt cctcgcccga
    10981 gtaccggacg tacgcgcccg gggccggcgc cgcggtcagc cgcacccgtc cgatcccgcc
    11041 gtcggtgtcc tcgacgggca ccacggcgtc agccccgggg ggcagcgcgg ccccggtcat
    11101 gatccgatgt gccgtaccgg gttcgagcgg cggaacgtcg agacgcccgg ccgggatgtc
    11161 ggcgctcacc ggcagctcga caggactcgc cgcaccgcct ccggccaggt cagcggcgcg
    11221 cacggcgtac ccgtccatcg ccgagttgtc gaacgacggc agggagacgg cggccacgac
    11281 gtcggcggcg agcacgcggc ctcgcgcctg ccggatcggg acgtcctctg cgggcatcgg
    11341 cggcagcagc gcggcgacca cggcgcggtg ctcttctacg gtcctctgga agctcgccac
    11401 gatcgcatcc tgcccgacct tgcggccgca cctcgtgacg gcgcaggcaa cgttttttgg
    11461 gtagcgtcga tcgggcgtag atcggcgagg aacatggagg tgccgtggct cagggcacgg
    11521 tcaagtggtt caacgcggag aagggcttcg gcttcatcgc catggatggc gggccggacg
    11581 tcttcgtcca ctacagcgca atccagtcag acggtttccg gacactggac gagggcgacc
    11641 gcgtggagtt cgagacgacg gcgggtcggg acgggcgcag ccaggctgac gccgtccgac
    11701 gcatctgagg aaagccgcct ccgcggcgac gctaccaccg gggggatcgg tcgtaccggt
    11761 ccccccgtag tcgtttcccc cgaacgcgtt ccgccgctcc gatcgcctcg tggcgacctt
    11821 gcactcgccg accccgagtg ctaaaaccgg gttggcactc ggcacggtag agtgccagat
    11881 gtcgagcagg gtgagaccgg cgcagggttg tcggtcgtcc gtcgcgggcg ccgggctcga
    11941 cgcgagactc tcgtgtcatc ccaatcggag gacaacaccc ccatggcgaa gatgatcgct
    12001 ttcgacgagg aggcgcgccg cgggctcgaa cgcggcatga acaccctcgc cgacgctgtc
    12061 aaggtgacgc tcggcccgcg cggccgcaac gttgtcctgg agaagaagtg gggcgcgccc
    12121 accatcacca acgatggtgt gtcgatcgcc aaggagatcg agctcgagga cccctgggag
    12181 aagatcgggg ccgagctcgt caaggaagtt gccaagaaga ccgacgacat cgcgggcgac
    12241 ggcaccacca ccgccacggt gctggcccag gccctggtgc gtgagggcct gcgcaacgtg
    12301 gccgccggcg cgaacccgat ggcgctcaag cggggcatcg agaaggccgt cgaggccgtc
    12361 tcgcagcagc tgctgaagac cgccaaggag gtcgagacca aggagcagat tgcggccacc
    12421 gcctcgatct ccgcggccga ctccaccatc ggcgagctga tcgccgaggc gatggacaag
    12481 gtcggcaagg aaggcgtgat cacggtcgag gagagccaga ccttcggtct ggagctcgag
    12541 ctgaccgagg gcatgcgctt cgacaagggc tacatctcgc cctacttcgt caccgacgcg
    12601 gagcgcatgg aggcggagct cgaggacccc tacatcctcc tcgtctcctc caagatctcc
    12661 acggtcaagg acctgctccc gctgttggag aaggtcatgc agaccggcaa gccgctggcg
    12721 atcatcgccg aggacgtcga gggcgaggcc ctggccaccc tggtcgtcaa caagatccgt
    12781 ggcaccttca agtccgtcgc cgtcaaggct ccgggcttcg gtgaccgccg cgaggcgatg
    12841 ctggccgaca tggccatcct cgccggtggc gaggtcatct cggagaaggt cggtctcaag
    12901 ctggagaacg ccgacctgtc gctgctgggc cgcgcccgca aggtcgtggt caccaaggac
    12961 gagaccacca tcgtcgacgg tgccggcgac gccgaccaga tcgcgggccg ggtcaaccag
    13021 atccgctccg agatcgagcg caccgactcc gactacgacc gcgagaagct gcaggagcgc
    13081 ctggccaagc tggccggcgg tgtcgcggtg atcaaggccg gagcggccac cgaggtggag
    13141 ctcaaggagc gcaagcaccg catcgaggac gccgtgcgca acgcgaaggc ggccgtcgag
    13201 gagggcatcg tcgccggtgg cggcgtggcg ctcatccagg ccggtgccgg cctgttcgag
    13261 ggcctcggcc tggacggtga cgaggccacc ggcgcgaaca tcgtcaaggt cgcgctggag
    13321 gctccgctca agcagatcgc catcaacgcc ggcctcgagg gcggcgtcgt cgcggagaag
    13381 gtgcgcgggc tcaacgccgg cgagggcctc gacgcggcca ccggtgagta caaggacctg
    13441 atcaaggccg gcatcatcga cccggccaag gtcacccgct cggcgctgca gaacgcggcc
    13501 tcgatcgcgg ccctgttcct caccaccgag gccgtcatcg ccgacaagcc ggagaggaac
    13561 ccggccccgg ccggcgaccc gtcgggcggc atgggcggca tggacttctg agtccggcca
    13621 ccccgcagta ccccgcacgt cccgcagggg cggcacccgg atcatcgggt gccgcccctg
    13681 cggcgttcgg gagcatgccg cagctgctgg ttgccggggg gttcagcggt ggtgtcctcg
    13741 ccccagcacg ctggaggtca gccggcaacg tagcgggcgg cggtggccgt tgcccgttcg
    13801 acgagcaccg cgcgtagctc cggaggcccg atcgcctcgg cgtccggacc gagtgcccag
    13861 accacgctca gagcgtggtc gagcgcggcg aactcgagat cgaggcgcag ccacccgtca
    13921 ccgtcgtccc gctcggcggc cacctcgcgc acggtgcgca ccaaggaggc cctgacgtcc
    13981 ggccgcacgc gaacggtcgc ggtaaggcct ggcagggtgg ccgcgaaccg cgcgcgtcgc
    14041 tcctcccacg tccgctgcag atccacgcct gcgggccggt cggcgggctc ggccagctcc
    14101 tcgacgtcca gcaagcgcga gaggcggtag gtccggtcgg cgccgtcgcg ggtggcgagc
    14161 aggtaccact ggccgcgcgc gctcacgagg cccaccgggt cgaccgtccg ccaccgcgtc
    14221 tcctccccgg acgcggcgta gtgaatgcgc agcttgcgcc ccgcgaatac cgccctgcgg
    14281 accacggcca gccgctcctc gtcgtcgggt gcggccgggc cgccccccgc gcgaaccatg
    14341 ccctcccgtc gcaccaggat gcgtcccgca tcgcgtgtgg cggcagtgcg ctgctgctgg
    14401 ggcagcgccg ccacgacctt gcgcatcgcc gaggccagcg caggtgccgt gcccggggag
    14461 ggcgccgcgg cctgcgagcc cgccaccagc agcgcgagtg cctcgtcgtg ggtcagcccg
    14521 gtgaggtcgg tggtgtagcc gggtaggagc gcgaacccgc cgtgccgccc ccgctcggcg
    14581 tacaccggaa cgcccgcggc cgagagcgcc tcgacgtctc gcagcaccgt gcgcgtggag
    14641 acctccagct caccggccag cgccgtggcc gacatccggc cgcggtgctg cagcagaagc
    14701 agcagggaca gcaggcggtc cgcgcgcacg agccgaagga tgtcaggaat acacgacaca
    14761 agacgtcgtg attcgcgtcc agactcgacg ccatgaccac caccgagatc accgatcccc
    14821 gcccgctcta cgccgcggcc ctctcctggg cccacgacgt ggtgcgcggc gtccgcccca
    14881 gccagctcga cgggcccacc ccgtgcacgg agttcgacgt ccgcacgctg ctcgcccacc
    14941 tcgtcgccac cgtcgaccgc gcggcagcac tcggcaaggg catcgacgcc gcgtccactc
    15001 ccagcttcgt ccccgccgca cccgacgacg gctgggggac ggcgtacggc gacgcgatct
    15061 ccgcgatgtg gacggtctgg cgcgacggcc ccggcagcga cgacctgctc gaccggaccg
    15121 tcaccgcccc gttcggcacc gtccccggcc gcgcggcggt gctggcctac acctcggaga
    15181 cccttgtgca cggctgggac ctggccgttg ccacgggcca ggacgccgag gccaaccccg
    15241 cggtggcgcg ggcggtgctc gcgatgatgc agcgctttct ccccgccgaa cctcgcggcg
    15301 gccgcatccc cttcgcgccc ccggtgccgc cccgcgacga cgcgggcccg acggagcagc
    15361 tcgcgaactg gtgcggccgg ggtcgcgcct gaccgccggc gcggccgacc gacggcactc
    15421 tcaccggatc cggcggtact ggcggagccg gtgggaggac ggcgcggccg cggcgagcag
    15481 gcggaccagt gagcagatca ccgcgctggc gggcggtgac cacgcgcacc tccgcgaagc
    15541 cgtcgtgtcg cgcatcttgc agcccgcacc ctacggcccg gtgcggattt gcggcggtcg
    15601 ggcgcgacca ccgggttggt tgacccgcat ctcaagccgc ccacagaggg gaaggcgctt
    15661 tcctccgtgc cgctgaacgg tacgctctgc ggacagggaa tcccggacca agccggacct
    15721 ggcctgtcgg gggccgcctc gtcggcgcgg ccagtccgct ccggccgtcc gaccccgtct
    15781 cgcgccacag gaggccggcc atgaacgagc gcgtcgcgac cgcgtcggtc acccttctcg
    15841 acgtcgcccg cgcggcgggg gtctcgctgg ccaccgcgtc acgagcgctc aacggcagca
    15901 cccgccgggt ccgccccgac ctgcgcgacc gggtcctcga ggccgccgac gccctgcagt
    15961 actcggccaa tgcgcaggcc caggccatgg cgcgcggcca taccaacctc gtcgggctcg
    16021 tgatcagcga tgtgggtgac ccctacttct cctccatcgc agcgggggcc atccgggcgg
    16081 cagaggacca ccagctcgtc gtcacgatgg cctgcacgtt cggccgggag gaacgggaga
    16141 tcgactacgt cgccgccctg cggagccagc gggcgcgcgc cgtgatcgtc gtggggagca
    16201 gggtgcacga ccgcgggctg acggaccggc tcggtgccga ggtgcgctcg ttcgagcagt
    16261 ccgggggccg cgtcgccttc atcagccagc gcaagatcgc ggccgacacg atcgtcctgg
    16321 agaaccgcgc gggcgccagg gcgctcgccg aggcgctgtg ctgccgcggc taccgccggt
    16381 tcgcgatact cgcaggtcgc gccgacctac tgaccgcgcg cgaccgttcc gcggggttcc
    16441 gcgagggcgc ggcgcgccac ggggtgcaga tcgcccagga cgatgtcgtg cacggcgggt
    16501 tcaaccgtga cggcggctac gacgcgatga tcgaggtcct cgaccgcggt cgcgacgtcg
    16561 actgcgtctt cgccgtcaac gacgtgatgg cgctcggcgc gatggccgcc ctgcgcgatc
    16621 ggggcgtggc gctgccgtcg gggatggcgg tggccgggtt cgacgacatc gtcaccctgc
    16681 gcgatgtcac ccccgcgctg accaccgtcc ggttgccact cgaggacttg ggcgcctggg
    16741 cgctgcagct ggtgctcgag ccgtccaccg accagccccg gatccggcgg gtgcgtggcg
    16801 aggtcgtact tcgggagagc acaccagtgc gcccctgacc ggaggccgac acccgacgaa
    16861 tggcgaattc cgccacgatg cgcagttctc gtcattcgtc ccggcgcggc tcggctgtcg
    16921 atcacgacaa atcaccggcg gcgatatgga gaagtgctag cgtcgaagtt gcgggacagg
    16981 aacggccccg ttctgccggg ccggcaccgg gcccacaggc gcgtgagctg gcctcacacc
    17041 ggtccattga ctgatctcgg gtagcacctg aatccctgcg gcctgtcggg aaaagattgt
    17101 gccgacgtcg ggcaagcgcg tcggccgaca acgacgacac gaggagaaca agcgtgacga
    17161 cgacgaaggc cgtgaccgtg atcggtaccg gcatcatggg agccgggatg gccaggagcc
    17221 tcgccagggc cgggctcacc gtgacggtgt ggaaccgcga cgcgggaaag gcccgtccac
    17281 tcgccgatgt cggggcgacc gtggccgacg atcccgcatc ggcggtggcc ggggccgacg
    17341 tcatcctcac catgctcttc gacgtcgagt ccgtcgagca ggtgatgggc caggcactgc
    17401 cggccgccgc agagggagcg gtctgggtgc agtccagcac cgtcgggctc gacggcgcgc
    17461 tccggctggc cgagctcgcc gaccgggccg gcatcgacta cgtcgacgcc cctgtcctgg
    17521 gcacgcggca gccggcggag aacggcaccc tcctcgtact ggccggtggg ccggagcggc
    17581 tgcgcgacac cgtggccccg gtgctcgatg cgatcggctc ccgcaccgtg tgggtcggcc
    17641 cccgacccgg tgacggccac cggctcaagc tcgtcgcgaa ctcgtgggtg ctgtccgtcg
    17701 tcgggggcac cgcgcaggcc atcggactgg ccgaagggct cggtctggac ccgcagctgt
    17761 tcctcgacac catcgccggt ggggccaccg actccgtcta cgcccaggtc aagggcagcg
    17821 cgatgatcgc cggcgacttc ccgccgtcgt tcaccctcga cggtgcggtc aaggatgccg
    17881 gactcatcgc ggacgccctc cgcggcgcag gcgtcgacga tcggctcgcg cgggcgctcc
    17941 accagcagtt cgcgagcgcc gccgaggcag gccacggcgc cgaggacctg gcggccgtgg
    18001 tggaggcggt ccgcaaccct ccatcgtcct gacgcgacat ccggccgcgg gtctacggtc
    18061 cgcggccgac cgtcatccca cgtcgggtat cgccggcccg aggagatcgt cggcgtcgac
    18121 gatgcggtag gcataaccct gctccgccag gaaccgctgc cggtgggccg cgtagtcggt
    18181 gtcaagcgtg tcgcgggaga cgaccgagta gaagtgggcc tgccggccgt cgcccttggg
    18241 tcgcagcagc cgaccgagcc gctgggcctc ctcctggcgg gagccgaacg tgccggaaac
    18301 ctggatggcg acgctggcct caggcaggtc gatggagaag ttggccacct tgctcaccac
    18361 gagcaccggt agctcacccc gccggaactg gtcgtacagc gcctcgcgct ccctgttctt
    18421 cgtggagccc tggatgatcg gcgcgtcgag cgcctcgccg agctcctcca gctggtcgag
    18481 gtaggcgccg atgaccagcg cgggctcccc ggggtgcttc ccgaggatgg ccttcacgac
    18541 ccccagcttc gtgtgagcgg tggaggccat cttgtagcgc tcctctgcct ccgcgaccgc
    18601 gtacccgagc cgctcggcgt cggtgagcgt gacccggacc tcgacgcagt cggcgggggc
    18661 gatccagccc tgctgctcga tgtcgcgcca gggagcgtcg taccgcttcg gaccgatcag
    18721 ggagaagacg tcgccctccc ggccgtcctc ccgcacgagt gtggcggtga gcccgagcct
    18781 gcggcgcgac tgcaggtcgg ctgtcatccg gaaaaccggc gcggggagca ggtgcacctc
    18841 gtcgtagatg acgagacccc agtcgcggga gtcgaacagc tccaggtgct tgtactcgcc
    18901 cttggtcttg cgcgtgacca cctggtaggt cgcgatcgtg accggacgga tctccttgcg
    18961 ctcgccggag tactcaccga tctcttcctc ggtgagcgag gtgcgtgcca ccagctcgcg
    19021 cttccactgc cgccccgcaa cggtgttggt gacgaggatc agcgtggtgg cctgcgcctc
    19081 tgccattgcg gccgcgccaa ccagcgtctt gcccgccccg cacggcagca cgacgacacc
    19141 ggagccgcct gcccagaagc catcgacggc ctggcgctgg tagtcgcgca gtgaccagcc
    19201 gtcctcggcc agcgcgatgg ggtgcgcctc accgtcgacg tagcccgcga ggtcctcggc
    19261 gggccagccg atcttcagca gggcctgctt gaggtgcccc cgctccgacg ggtgtacgag
    19321 aaccgtgtcg tcgtcgaccc gggtgccgag cagcggggcg atcttcttct gccggagcac
    19381 ctcctccagc accgcgcggt ccagtgcgac catgaccagc ccgtgcaccg gcgagttcgt
    19441 cacctgcagg cgcccgtacc ggcccatcgt gtcgacgacg tcgacgagga gcggctgcgg
    19501 caccgcgtag cgggagaagc ggacgagcgc gtcgaccacc tgctcggcgt cgtgccccgc
    19561 cgcgcgcgcg ttccacagcg cgagcggcgt gacccggtag gtgtggacgt gctcgggggc
    19621 acgttccagc tccgcgaacg gcgcaatggc tgctctcgcc tccgcggcgg ccggatggtc
    19681 gacctcgagc agcagcgtct tgtcggactg gacgatgagc ggcccgtcgg tcatatgtgt
    19741 cgtactcctc acaacgaccc ggccactgcc gggcctgaac tggacgtgcg gcatgctcgg
    19801 gtacgcgccg gatctgggga acgctgccgc ccggcctcga cgaagggggc attgccgact
    19861 tcggctatgc ttcgcccgct ctgtccgaga gtgcacgtgt gccaccgcga tcgtaccggg
    19921 tcctggaggt cgtcgtcctt ccccagaggg cgcgtgtcgg agtcggcgga gcgcgcacga
    19981 cgcgggtcgg cacacccgac cggcccgccc ccgacagaag gcgcgacgga tcgacccgat
    20041 gctcatcgca atgctccccg ggcctggcac ccgttcccgg ccacccaccg cccctgggcc
    20101 gcagctcatc cggtccgggc tcgcgggcga tcaccagggc gtgtcagagt tcgctcgatc
    20161 gtcatcggca atcgtcaggg ggacgttgac gtgaccagca tcgagaccgg gcccacctgg
    20221 tccgagcgat tcaacccgcg caactggacg ctggcctgga agctggtgat cgtctgtctg
    20281 atcccggccc tgctggcact cgccctcggc gtcctgcggg tgtccgacca ggcaggtcag
    20341 gcggccgatc tcgggcgcag cacccggctg ctggaggtgg cgcagcgggt gtcgggcgcc
    20401 gccgacacac tgcgccagga gcgtaacgcg tccacgatct tcgccgcggg ccgccgcacc
    20461 gacgatcgga acgccctcga ggccggattc cgccagaccg acggcgtgct cgacgagacg
    20521 ctcggcgccg tccacagcac caccgggctc gaggtcacca cgcaaagcgc gctgcagaag
    20581 gccgagcgtg agttggcgac gctggggcct ttgcgcaccg acgccgccca gaacccggcc
    20641 gtcagctccg atgtgatcag ctcgcgctac accaccgtga tcgacaccgt cgacgtcctc
    20701 ggccgagccc tgctgcgcca gctgagcacg cccggcatcg ggggcctccc cgacgccctc
    20761 accgccgtcg tgaacatcag cgagcagctc gcgatccagc acacggtgct gagtgccgcc
    20821 atcaggtccg gcagcctccg gcccacggac gtcagcgcgg tgaactccac cgacagccag
    20881 ctcgatgccg ccgcccgcga gtaccagacg gcgctcaccg cgggtgtggt cggcaaccca
    20941 ctcgacgcca ccgccaacac ccagcgcgag ctactgaaaa gcgcgatcct ggccaccccg
    21001 cccgcgcggc cgatcggcgt cacgctcgca gcatgggacg cggcccacgc cgacgcgcag
    21061 gccgcggccg accgggcgtc cacccgcatc cgcaaggcgc tgtcctccgc gagtgccgcg
    21121 gccgaggagc gagccagcaa cgaggccggc ctcaactccg tggtcctgat gctcggcctg
    21181 ctcatcggtg ccacgatcgc cgttctcgtc tccagggccc tgctccggtc gctgcaggtt
    21241 ctgcgcacca ccgccctcga cgtcgccgaa cggcggctgc cacaagcagt cgagggcatg
    21301 cgcgctggcg agacgcccga cgtcgaggtc gatccggtgc ccctgtcggg ccgcgacgag
    21361 gtggctcagg tggcccgggc gttcgacgcg gtccatgggc aggcggtccg cctcgcggcc
    21421 gagcaggcgg ccctgcagtc gaacgtcagc gcgatgttcg tcaacctctc ccgccgcagc
    21481 caggcgctcg tcgagcgcca gctgcagctc atcgagcagc tggagaccaa cgagcaggac
    21541 cccgaccagc tgtccaacct gttccagctc gaccacctcg ccacccgcat gcggcgcaac
    21601 agtgagaacc tgctggttct cgcgggcacg gacctcgcca agcggaacat cgcgccgatc
    21661 ccgatggtcg acgtgctgcg cgctgcggtg tcggaggtcg agcagtacca gcggatcgtc
    21721 gtgcagacac cgcccacggt caccgtggtg ggccgggcgg cgtccgacct cgtccacctg
    21781 ctggccgagc tgctcgacaa cgccaccagc ttctccccgc ccgactcgca ggtggtcatg
    21841 agcaccgccc gcacggccga cgggtcgatc gtcgtcgaga tcgccgaccg cggcgtcggc
    21901 atgatcgacc acgagctcag cgacgccaac cagcggctgg gcgggccatc ctccgtcgac
    21961 gtctcggcgt cccgccgcat gggtctgttc gtggtcggtc gcctgggcgc ccggcacggc
    22021 atcagcgtgc ggctcagcac gtcgtctgca ggcggtcccg gtgcgggcct caccgcatcg
    22081 gtcacggtgc cggcctacct gatcccgtcc agcgagcccg ccgagacgcg tcggcagccc
    22141 ggtgcactcc ccgtccgtgg caacgcactg ctgccgccgc gcgcgggcac caacggcaat
    22201 gctcgtcccg gatcgctgtc ggcgctggta gcgggcaccg acggcccggc caacccggcc
    22261 gggatcttcg acgcaccggc cgggaaggac gggcagctgt tcgcccctca gccgcgcccc
    22321 accatgaacg gctcgtcggg caagctgccc acccggcgac ctggctccac cgtgcgaccc
    22381 gagggcgcat cctccacgcg gccggatccg gccgagcagg cagcagccga ccaggccgcg
    22441 gcggagcagg cgcaggagaa ggcccgggag gtcgccgcgc aggcggccgc cgacgccgag
    22501 ctcgccgaac agcagcagcg cgaccaggcg cagcgctccg cggcacaggg ccagcaggcc
    22561 ggcgcccacg ccaccgggtt ctaccgcaac ggtgcccagc ccgacaccgc ccgctcgttg
    22621 tggtcgaccg gcggcgacaa gtcgtcggcc aacggcttcg tgccgaacga tgcccccgcg
    22681 gccaacggga gcactccgca cggggccgtc acgaacggct ccgctcccga gggcacgcag
    22741 tccgacgacg ggcccacgcc tcgccagcag cctgctgcga gcagcgaggc ggagagcacc
    22801 tccggggccg aggcggcggc agaccagtcc ggatcgggcc aggacaggcc cgacgagcgg
    22861 tctcccgacg cacagcagac cggcacgttt cctgcggaga ccgcgttcgg caacggggcc
    22921 cagacacaac gtggcgctcc cacccaggta ccaccgtccc cgcccaccat gccgatgccg
    22981 gtcgtcggac gcggtgcttc gatgccgccg cctcgacccg ccgttcagcg ccccgagcga
    23041 cccgcccagc agcaggccgc tccgcgaccg ttcgcccaga gtccggggtc ggccgagcac
    23101 acccagcagc agccggtcca gcacgctcag ccggcccagc ccgctgaatc tgcacagccg
    23161 gcccagcgcg ctcagccggt ccagcccgct gaatctgcac agccggccca gcacgctcag
    23221 ccggtccagc ccgctgaatc cgcacagcct gcccagcacg ctcagccg
//
LOCUS       DASRSC010000140         4742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2339115,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000140 DASRSC010000000
VERSION     DASRSC010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2339115"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(566..2860)
                     /locus_tag="VFE39_06730"
     CDS             complement(566..2860)
                     /locus_tag="VFE39_06730"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET6258329.1"
                     /translation="MTTVLPAAAPETVDTELLVEGMTCGACSARIERALRKLDGVQAN
                     VNYATGRAAVTHAGTVTVEDLVTAVERAGYRARQPEPGTRDDEAEAAHARELAALGQQ
                     LAACAALALPVIVLSMVPAIQFPFWQWTALGLTAPVALWGALPFHRAALANLRHATAS
                     MDTLVSVGTLAAFGWSIYAMVFGTAGRPGLTHSFDLTLAPTDGAGHIYLEVAAGVTTF
                     VLAGRYLEARARRRSGAALRGLLALTATDARVLRDGREHLVPVDRLAVGDTFLVRPGE
                     KVATDGVVIDGSCALDTAAITGEPTPADVAPGDDVIGGCVALGGRLVVQATRVGADTQ
                     LAQTAALVERAQTGKAAVQRLADRVCGEFVPVVLMLAAATWGFWTGAGADAATSFAAA
                     TAVLIVACPCALGLATPTALLVGTGRAAQLGVLVTGPAVLESTRRVDTIVLDKTGTVT
                     TGVMTVTDVVPADGEDRDEVLVVAGALAGTSAHPVAQAVARHAADRAAAPAPEVRDVV
                     SLDGLGMQGVIGDVEVLLGRPRLLQERGIPIPGPLQEPLADAAANGRTTVAMAWAGRA
                     RAVLVLSDTIRPTSTDAIAELRRLGLDPVLLTGDGMGAARAVADTVGITTVVADALPT
                     DKVELIARLQAQGRVVAMVGDGINDAAALAQADLGIAMGTGTDIAISAADITLVRADL
                     TAAVDAVRLSRRTLAVIKANLAWAFGYNILALPVAAAGMLHPMLAGAFMAASSVLVVT
                     NSLRLAGRGRRTSTASTATAAARS"
     gene            complement(2857..3102)
                     /locus_tag="VFE39_06735"
     CDS             complement(2857..3102)
                     /locus_tag="VFE39_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HET6258330.1"
                     /translation="MTEATTRPRQNRVTTTLVISGMNCDSCAQHVQGALAALPEVTAR
                     VDLAASTAEVHHPSTVSLDTVVAVVRDAGYTAEPARR"
     gene            complement(3135..3311)
                     /locus_tag="VFE39_06740"
     CDS             complement(3135..3311)
                     /locus_tag="VFE39_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258331.1"
                     /translation="MDASPAQPAPPSVTRANAARVVAFLAVLALTAAGGWQAGRILEP
                     PRPAPVPATTHGQP"
     gene            3364..3570
                     /locus_tag="VFE39_06745"
     CDS             3364..3570
                     /locus_tag="VFE39_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258332.1"
                     /translation="MNTSDGPQRVRDRHHTGRGDHDERTAGDGVVLDLGSGGWSFVIS
                     SQARVTAVTSEVVLRLNGSHWCSP"
     gene            complement(3768..>4742)
                     /locus_tag="VFE39_06750"
     CDS             complement(3768..>4742)
                     /locus_tag="VFE39_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HET6258333.1"
                     /translation="LHRHLLLGEPGRLYSELAASWLWVVALGGLALWIARWRARRGAG
                     LAEARDLIRPESGATGRRRTLSRHGALGVWLVLGALFLSATGLTWSTYAGANVTALRA
                     SLGWSTPSLQTRLSAAHAGATPAATGDLTGDPDRFGAVLATARAAGINAGLVEIARPT
                     EAGTAWTVTEIERSWPTQVDAVAVDGDTGSVVAEVRFSDYPLVAKLARWGIDAHMGVL
                     FGWVNQLVLVAFGLGLITLIVLGYRMWWRRRPTRGRGGRPVPRGQMRRATQATAFVAV
                     LLAVAIGWFAPVLGVSLLAFLMIDGLLGWRTRNRASKLNDEPGELLDA"
BASE COUNT          664 a         1661 c         1778 g          639 t
ORIGIN      
        1 caagcgcgac gacctgatca atccaaagat tccggggaac aagtggcgca agctgaagta
       61 caagctggac gacgcggccg cggttggtca cccccgctgc tgaccttcgg tggcgcctac
      121 tcgaaccaca tccgcgccct cgccgctgct ggccggttct tcggcttcga caccgtggga
      181 gtcatccgcg gtgaggagca cctcccgctg tacccgacgc tcgcctctcc gtcagccgcg
      241 gaatgaccct gttccacctc gaccgcgaga ccctgcccgc ggagctggac ttcgactacg
      301 acgtcctctg ctgctccgtt ggtacgggca gaacactggc ggcatcgcgg cgggcgctcc
      361 acacggagtc aaggtcctcg gcttcggcaa gatgctctgc gggctctacg acctgatcga
      421 caagggcagg ttccagccgg gcaccaccgt ggtcgccgtc gtgactgggc cggcgtcatt
      481 gccagctccg acccggtcgg gtgcgacagg ctctcgacgg cgagcgacgt gccctgctgg
      541 cggataacga cgaagtgcgt ggacgtcagc tcctggccgc ggccgtcgct gtagaagctg
      601 tactcgtccg acggccccgg ccggcgaggc ggaggctgtt ggtgacgacg aggacggaac
      661 tggcggccat gaacgctccc gcgagcatgg ggtgcagcat tcccgcggct gcgacgggta
      721 gggcgaggat gttgtagccg aaggcccagg cgaggttggc cttgatgacg gcgagggtgc
      781 ggcgggagag ccggacggcg tcgacggctg cggtgaggtc ggcgcggacg agtgtgatgt
      841 cggcggcgga gatcgcgatg tcggtgccgg tgcccatggc gatgccgagg tcggcctggg
      901 ccagcgcggc ggcgtcgttg atgccgtcgc cgaccattgc gacgacccgg ccctgtgcct
      961 gcagccgggc gatgagctcg accttgtcgg tcggtagcgc gtcggccacg acggtggtga
     1021 tcccgaccgt gtcggcgacc gcgcgtgcgg cgcccatgcc gtcgccggtg aggagcacag
     1081 ggtcgagtcc gagacgacgc agctcggcga tcgcgtcggt gctggtcggt ctgatggtgt
     1141 cggacagcac gagcaccgcg cgagcgcggc ctgcccaggc cattgccacg gtggtgcgtc
     1201 cgttcgccgc ggcgtccgcg agcggctcct gaagggggcc ggggatcggg atgccgcgct
     1261 cctgcagcag gcggggcctg ccgagcagga cctcgacgtc gccgatcacg ccctgcatac
     1321 cgagcccgtc cagggagacg acgtcgcgca cctcgggggc gggggctgcg gcgcggtcgg
     1381 cggcgtggcg ggcgaccgcc tgtgccacgg ggtgggccga cgtgccggcc agtgccccgg
     1441 ccacgacgag tacctcgtcg cgatcctcac cgtcggccgg cacaacatcg gtcacggtca
     1501 tcacgccggt cgtgaccgtg ccggtcttgt cgagcacgat ggtgtcgacc cgccgggtgg
     1561 actcgagtac cgccggcccg gtgacgagca cgccgagctg cgcggcgcga ccggtgccga
     1621 ccagcagcgc agtgggcgtg gcgagtccga gcgcgcaagg gcaggcgacg atcaggacgg
     1681 ccgtcgcggc cgcgaaggac gtggccgcat ccgcgccggc gccggtccag aacccccagg
     1741 tggcggcggc gagcatgagc accacgggca cgaactcgcc gcagacccgg tcggcgaggc
     1801 gttggacggc ggcctttccg gtctgcgcgc gctcgacgag cgcggccgtc tgcgcgagtt
     1861 gggtgtccgc cccgacgcgt gtcgcctgca cgacgaggcg ccccccgagc gcgacgcacc
     1921 cgccgatcac gtcatcgccc ggtgcgacgt cagcgggtgt gggttccccg gtgatcgccg
     1981 ccgtgtccag cgcgcaggag ccgtcgatca ccacaccgtc ggtggcgacc ttctcacccg
     2041 gccgcaccag gaaggtgtcg ccgacggcga gccggtcgac gggcacgagg tgctcccgcc
     2101 cgtcgcgcag gaccctggcg tccgtcgcgg tgagcgcgag caggccgcgc agggcggcgc
     2161 cggagcgccg gcgggcccgg gcctccaggt agcgcccggc gagcacgaac gtcgtcaccc
     2221 cggcggcgac ctcgaggtag atatgacctg cgccgtcggt cggggccagc gtgaggtcga
     2281 aggagtgggt gaggccgggc cggccggccg tgccgaacac catggcgtag atcgaccagc
     2341 cgaacgccgc gagggtcccg accgacacca gggtgtccat ggaggcggtc gcgtgtcgca
     2401 ggttcgccag cgccgcccga tggaacggca gcgcgcccca cagcgcgacc ggcgcggtca
     2461 gcccgagtgc cgtccattgc cagaacggga actggatggc gggaaccatc gacagcacga
     2521 tcaccggcag ggcgagcgcc gcgcaggcgg cgagctgctg ccccagcgcg gcgagttcac
     2581 gcgcgtgggc cgcctcggcc tcgtcgtccc gcgtgcccgg ttccggttgc cgggccctgt
     2641 agccggcgcg ctcgaccgcg gtcacgaggt cctcgacggt cactgtgccg gcgtgcgtga
     2701 cggcggcccg accggtcgcg tagttgacgt tcgcctgcac accgtcgagc ttgcgcagcg
     2761 cccgctcgat gcgcgccgaa cacgctccgc acgtcatgcc ctcgacgagc agttcggtgt
     2821 ccaccgtctc cggggcggcc gccgggagga ccgtggtcat cgtcgggcgg gttctgcggt
     2881 gtagccggcg tcccggacca ccgcgacgac ggtgtcgagt gaaacggtgg agggatggtg
     2941 cacctcggcg gtcgaagcgg ccaggtcgac ccgggccgtc acctccggga gcgcggcgag
     3001 cgcgccctgg acgtgctgtg cgcagctgtc gcaattcatg ccggagatga cgagcgtcgt
     3061 tgtgacgcgg ttctggcggg gacgggtggt ggcttcggtc atggggatct cctcgtgcgg
     3121 tgtcggctcg gtcgtcacgg ttggccgtgc gtggtcgcgg gaaccggcgc cggccggggc
     3181 ggctcgagga tgcggccggc ctgccacccg cccgccgcgg tgagggcgag cacggcaagg
     3241 aaggcgacga cgcgtgcggc gttcgcccgg gtgaccgaag gcggagccgg ctgggcgggg
     3301 gatgcgtcca cggcggcaac ttatggcagc gccccaacga cggccaacca tccgtacccg
     3361 ctcgtgaaca cctcggacgg cccccagcgc gtacgcgacc gacaccacac cggacgcggc
     3421 gaccacgacg agcgcaccgc gggcgacggt gtggtgcttg atctcggctc cggcggatgg
     3481 tcgttcgtga tctcgtccca ggcccgcgtc acggccgtca cgtccgaggt cgtgctgcgc
     3541 ctgaacggct cccactggtg ttctccgtga cggccacgaa gcctgatgag tcgcgaagcg
     3601 gtgcccgttg atgcgggcgc tgtgatctca tcgagccata cagatcgcag cgttcgactc
     3661 ttcggctgat cggaggtctc gcggcgcggg tggaaccctg ctgggccgag gggtggagtg
     3721 caagatacgt cttcccgcct tgcaccccac ccctcgggcg tcctcgatca ggcgtcgagc
     3781 agctcgcccg gctcgtcgtt gagcttcgag gcacggttgc gggtgcgcca cccgagcagc
     3841 ccgtcgatca tcaggaacgc gagcaggctg accccgagca ccggcgcgaa ccagccgatc
     3901 gcgacggcca gcagtaccgc gacgaacgcg gtcgcctgcg tggcccgccg catctgtccg
     3961 cgcggtaccg gccgcccccc acggccccgg gtaggccggc ggcgccacca catccggtag
     4021 ccgagcacga tcaatgtgat cagcccgagg ccgaacgcca ccagtacgag ctggttgacc
     4081 cagccgaaca gcacccccat gtgcgcgtcg atgccccagc gggcgagctt cgccaccagc
     4141 gggtagtcgc tgaaacgcac ctcggcgacc acgctgccgg tgtcgccgtc gaccgcgacg
     4201 gcgtcgacct gggtgggcca gctccgctcg atctcggtga cggtccacgc cgtccccgcc
     4261 tcggtgggcc gggcgatctc gaccagcccg gcattgatgc cggcggcccg ggcggtggcg
     4321 agcaccgcgc cgaagcggtc ggggtcaccg gtgaggtccc cggtcgccgc cggggtagcg
     4381 ccggcgtgcg cggcggagag cctggtctgc agggaggggg tgctccagcc cagcgatgcg
     4441 cgcagcgcgg tgacgttcgc cccggcgtag gtcgaccagg tcaatccggt ggcggacagg
     4501 aacagcgcgc cgagcacgag ccagaccccg agcgcgccgt gccggctcag ggtgcggcgc
     4561 cggccggtcg cacccgattc cggacggatc agatcgcggg cctcggccag gcccgcgccg
     4621 cggcgggctc gccaccgcgc gatccacagc gcgagcccgc cgagcgcgac cacccacaac
     4681 caggacgcgg cgagctcgct gtagagccgt ccgggctctc cgagcagcag gtggcggtgc
     4741 ag
//
LOCUS       DASRSC010000141         8775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3648066,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000141 DASRSC010000000
VERSION     DASRSC010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8775
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3648066"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VFE39_06755"
     CDS             <1..976
                     /locus_tag="VFE39_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET6258334.1"
                     /translation="VEVLDASPWRVPTSCSWARAGGCGGCDLQHADPAAQRSLKADVL
                     AEQLRRLAGIERPVTVEELPGGALGWRHRVRLAVDDQGRAGLRAHRSHDVLPIADCPL
                     TPAGTLPPVLEHGFDPGSEVEVAVDADGLVHVGDDGPVVQRAAGREWLLSPGVFWQVH
                     PALPDTLAAVVGEWAQAPRGGTAWDLYGGVGLFAAVLAGQVGFDGEVTVVESSRQAVA
                     DGKAALADLPQVGWRVGRVERVLGGLGARPDVVVADPPRRGLGRELVDALCAQAPDRV
                     VHVACDPAALARDVALFAARGYRLEQLRAFDAFPMTQHFECVALLVRD"
     gene            1065..1583
                     /locus_tag="VFE39_06760"
     CDS             1065..1583
                     /locus_tag="VFE39_06760"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6258335.1"
                     /translation="MLIVLAGLPASGKSTIARALAVRLPAAVVAVDPIDDAMRRAGIG
                     ADQPTGLAAYMVAEAVAEDVLAAGLPVVVDAVNAVEPARQQWRRLAARRGVPMVVVEV
                     VCPDPGAHRRRLEGRSRGLTDLPEPTWDHVSRRAAEYEPWTEPVLRLDSALPVDANVE
                     VVLRELAGPDRR"
     gene            1591..2451
                     /locus_tag="VFE39_06765"
     CDS             1591..2451
                     /locus_tag="VFE39_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HET6258336.1"
                     /translation="MDLAEHLTGWAPEPVETTARIDVWPSGAFAALLDEPSPVRGAGD
                     PLPPLWHGFHLLDHPAQADLGDDGHPAHGRFLPPLPDRRRMFAGGRLEVVAPLRVDDL
                     VTRRSSLVSATPKSGRSGAMVFVTVRHEYDRGGVLAVVEEHDFAYRSQPAGVARGLPP
                     TAPVAPEPAAGEPGPWQVRLDPDEAVLFRFSALTYNTHRIHYDHPYVTHVEGYPGLVV
                     HGPLLALLLLELPRRFAPERRVTGFSYRLQRPAFAGAPVVAVGAPHGGDLTVGVPGQP
                     PSASGTVTFA"
     gene            complement(2553..4511)
                     /locus_tag="VFE39_06770"
     CDS             complement(2553..4511)
                     /locus_tag="VFE39_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HET6258337.1"
                     /translation="MTAVEDASVTITPAAVAGHPLEPLSGTEVAAAAEALRAAEGLAE
                     TARFVFVTLHEPPKAAVFAWSPGDEPLPREAHVVLYERSERTTYEAVVSLSDRAVVSF
                     RAIEGVQAPIMAEEFMSCEKIVQADPRWQAAMRRRGIEDFSLTMVDPWASSWTGPQDD
                     PSVRRIARPLTWVRSAPGENGYARPVEGLVVVVDLNAGEVVEVDDHGVVPIPEQPGNY
                     EEPWVFEPGNVPAFDAYRDDVKPIEITQPDGPSFTVDGHAVTWQKWHLRIGFTPREGL
                     VLHQLGYAGRPIVYRASLAEMYVPYGDPAPTHRFKNVFDQGEYGVGWLANSLTLGCDC
                     VGHIQYFDGVVSDNDGAPVIIPNAVCMHEEDAGIAWKHTDFRTGAVQVRRRRRLVIST
                     IVTVGNYEYGYFWYLYNDGLIEYEVKLSGVISTGAIAPGETPRHGTLVAPGLYGPHHQ
                     HFFCVRLDMSVDGRKNTVVEVDSAPSPPGPDNPHGNAWQVHRTVLGSEAVAQRETDPA
                     RARYWRIESAERTSALGAPTAYALMPGSTVPPMYSPDAVYAKRSGFTEHQLWVTAHDE
                     SQRFAAGDYPNQHPGGQGLPAYVAGDRPLQDTEVVVWYTFGAHHVVRPEDWPVMPVST
                     VGFMLKPSGFFDGNPALDLPPSAAHCHS"
     gene            complement(4508..6067)
                     /locus_tag="VFE39_06775"
     CDS             complement(4508..6067)
                     /locus_tag="VFE39_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6258338.1"
                     /translation="MTAVPTETNALPAESGKPGQLADGALGLPSVLFCIVTGAAPLAA
                     MLFNVPVATLGGGYASPAAFAVATVALTIFSVGYIAMCRRVTSAGGFYTFVTRGLGRV
                     LGLGSGLLIVLCYMVFAAAVTGTMGYFASTTVASFTGLDLPAWVYMIIGLALMSAFAV
                     FHIELTAKVLGVALIAELLVLVVLAVGVFAAGGAEGFSLAPLNPLNIFDNSAAIQVFG
                     AGAAGVALFAAFWSWVGFEMAPNYAEETRDPHRIAKAATYGSVIGLGLFYILISYVFV
                     TGWGLNGSAEAVKSQYAGQIASAFYPLTDRYVGAWATALMEVLIVTGSFACAMAFYNT
                     SARYLFALGREGVLPSALARTSTRHSPVTASMTVTVLVALYCLAFVIYDPTNEGALLK
                     LGTWSPLLGVLGILGVQALVSVAIIRYFLTAARDGFRWWSTLVAPALGFAAMTGACVL
                     LVVNRYDLAGAGDAAYIRLLPWVVLAVFLGGMGVAVVLRSRYPSRYARIGQFEVTEIG
                     DSTDTPQGAHA"
     gene            6291..>8775
                     /locus_tag="VFE39_06780"
     CDS             6291..>8775
                     /locus_tag="VFE39_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HET6258339.1"
                     /translation="MTSTPLPTQQKPTQQHSIQQRIADELGVRPHQVASAVDLLDGGA
                     TVPFIARYRKEATGTLDDAQLRTLEERLRYLRELEERRTVVLDEIRSQGKLDEALEAR
                     IMAAESKARLEDIYLPFKPKRRTKAMIAREAGLEPLADTLLADPSQDPAGVAAGFVNE
                     QVADVAAALEGARSILVERFAEDADLIGGLRERMWTRGRLVTKVREDKETEGAKFADY
                     FDFSEPFTKLPSHRILAAFRGEKEEVLDVALEPEDGTEPAEPGAVTDYERIIATAVGV
                     SDQGRPADKWLRDAVRWAWRTRILLHLGIDIRVRLRTVAEEGAIGVFATNLRDLLLAA
                     PAGSRATMGLDPGLRTGVKVAVVDATGKVVTTDTIYPHEPRRDWKGALATLTRLAAAH
                     DVELIAVGNGTASRETDQLARELAAKNPALKLIPVMVSEAGASVYSASAYASAELPEL
                     DVSLRGAVSIARRLQDPLAELVKIDPKSIGVGQYQHDLAESALSRSLDAVVEDCVNAV
                     GVDVNTASAPLLRRVSGITEGLATQIVLHRDANGPFRTRRALADVPRLGPKAFEQCAG
                     FLRIRGGDDPLDVSGVHPEAYPVVHRILERAKTDIGSLLGNAQKIKSLRPHEFVDGTF
                     GLPTVTDILAELEKPGRDPRPAFRTATFAEGVHKISDLQPGMLLEGQVTNVAAFGAFV
                     DVGVHQDGLVHVSAMSKQFVSDPRDVVKSGDVVRVKVLEVDEARKRISLTLRLDDEVG
                     AKPSRGDQRGGDQRRPERGPRDQKGGRDEKGGRDQKGRDQKGGDQKGGARGDQRVRGG
                     DRRGGGGRQGGSGGSGGGLGNSAMADALRR"
BASE COUNT         1262 a         3065 c         3243 g         1205 t
ORIGIN      
        1 ggtcgaggtg ctggacgcgt cgccgtggcg cgtgccgacc tcatgcagct gggcgcgggc
       61 gggtggctgc gggggctgcg acctgcagca cgccgacccg gccgcgcagc gttccctgaa
      121 ggccgacgtc ctggccgagc agctccgccg gctggccggg atcgagcgcc cggtgacggt
      181 cgaggagctg cccggtgggg cgctgggctg gcgccaccgc gtccggcttg ctgtcgacga
      241 ccagggcagg gccgggctgc gcgcgcaccg gagccacgac gtgctgccga ttgccgactg
      301 cccactgacc cccgcgggca cgctgccacc ggtgctcgag cacggcttcg acccgggttc
      361 ggaggtcgag gtggcggtgg acgcggacgg gctggtccac gtcggtgacg acggcccggt
      421 cgtccagcgc gccgccgggc gcgagtggct gctgtcccct ggagtgttct ggcaggtcca
      481 cccggcgctc ccggacacgc tggcggccgt ggtgggggaa tgggcccagg cgccgcgggg
      541 cggcacggcg tgggacctct acggcggggt cgggctcttc gccgccgtcc tcgcaggcca
      601 ggtcggattc gacggcgagg tcaccgtcgt cgagtcctcg cggcaggccg tcgcggacgg
      661 caaggccgcg ctcgccgacc tgccgcaggt gggctggcgg gtgggtcgcg tcgagcgcgt
      721 tctgggcggg ctgggcgcgc gcccggacgt cgtggtggcc gacccgcccc ggcgcgggct
      781 cggccgcgag ctcgtcgacg cgctgtgtgc ccaggccccc gaccgggtgg tgcacgtggc
      841 gtgcgatccg gcggcgctgg cgcgtgacgt cgcgctgttc gccgcgcggg gttaccgcct
      901 ggagcagcta cgggccttcg acgcgttccc gatgacgcag catttcgagt gcgtggcgct
      961 gctggtgcgc gactgatcgg cgcgggccct acctgcgcgc ccttcgtcgg gccgcgatga
     1021 acgatcgggg cgatcatgac ggtcacccgt gcgatggtgc cgggatgctg atcgtgctgg
     1081 cggggttgcc ggcgagcggg aagtcgacga tcgcacgagc cctcgcagtc cggctgcccg
     1141 cagcggtggt tgccgtcgac ccgatcgacg acgcgatgcg ccgggccggg atcggggccg
     1201 accagcccac gggccttgcc gcgtacatgg tcgcggaggc ggtggccgag gatgtcctcg
     1261 ctgcagggct ccccgtggtg gtcgacgccg ttaacgccgt cgagcccgcg cggcagcagt
     1321 ggcggcggct cgctgcgcgg cggggtgtgc cgatggtcgt ggtggaggtg gtctgtccgg
     1381 acccgggggc acaccgcagg cggctggagg gccggtcccg cggcctcacc gacctgcccg
     1441 aaccgacctg ggaccacgtg tcccgtcgtg ctgccgagta cgagccctgg accgagccgg
     1501 tgcttcgact cgactccgca ctccccgttg atgccaacgt cgaggtggtg ctgcgggagt
     1561 tggccgggcc ggatcggcga tgatcggggg gtggacctcg ctgagcacct gaccggatgg
     1621 gcccccgagc ccgtcgaaac cacggcgcgc atcgacgtct ggccttccgg ggcgttcgcc
     1681 gccctcctgg acgagccgag cccggtgcgg ggagcgggcg acccgctgcc gcccctgtgg
     1741 cacgggttcc acctgctcga ccaccccgcg caggccgacc tcggcgacga cggccacccc
     1801 gcccacggcc ggttcctgcc gccgctgccg gatcgccgcc ggatgttcgc cggtggccgg
     1861 ctggaggtcg tcgccccgct gcgcgtcgac gacctcgtca cccgccgctc ctcgctcgtg
     1921 tcggcaactc cgaagagcgg gcgcagcggg gcgatggtgt tcgtgacggt gcgccacgag
     1981 tacgaccgcg ggggggtgct cgccgtcgtg gaggagcacg acttcgccta ccgctcccag
     2041 ccggccggcg tggcccgcgg gctgcccccg acggcacccg tcgccccgga gccggccgca
     2101 ggtgagcccg ggccgtggca ggtgcggctg gatcctgacg aagcagtgct cttccggttc
     2161 agcgcgctga cctacaacac gcaccgcatc cactacgacc acccgtacgt cacgcacgtc
     2221 gagggctacc cggggctcgt cgtccacgga ccgctgctcg cgctgctcct gctggagctg
     2281 ccgcgccggt tcgcccccga gcgccgggtc accgggttct cctaccgctt gcagcgaccc
     2341 gcgttcgcag gcgcgccggt cgtcgccgtc ggcgccccgc acggcggtga cctcacggtg
     2401 ggggtgcccg gacagccgcc gtcggcctcg ggaacggtga ccttcgcctg actcgcacag
     2461 cagcgcggtc gggccgcgtc agccgcgcac gcgatgtgga ggaggccgtg cgcggatacg
     2521 agtgctccag cactcgtatc cgcgcacgag cgtcagctgt ggcagtgggc cgccgacggc
     2581 ggcaggtcga gggcggggtt gccgtcgaag aacccgctgg gcttgagcat gaagcccacc
     2641 gtgctgaccg gcatcaccgg ccagtcctcc ggacgcacga cgtggtgggc gccgaacgtg
     2701 taccagacga cgacctcggt gtcctgaagc ggtcggtcgc ccgccacgta tgccgggagg
     2761 ccctgtccgc cggggtgctg gttcgggtag tcgcccgccg cgaagcgctg gctctcgtcg
     2821 tgcgccgtga cccacagctg gtgttcggtg aacccggacc gcttggcgta cacagcatcg
     2881 ggcgagtaca tcggcggcac tgtggacccc ggcatcaggg cgtacgccgt gggcgcgccg
     2941 agggccgagg tccgctccgc gctctcgatc ctccagtacc gcgcccgtgc gggatcggtc
     3001 tcgcgttgcg cgaccgcctc gctcccgagc acggtccggt gcacctgcca ggcgttgccg
     3061 tgcgggttgt ccgggcccgg cgggctgggg gccgagtcga cctcgaccac tgtgttcttc
     3121 cgcccgtcca cgctcatgtc gagccgcacg cagaagaagt gctggtggtg tggcccgtac
     3181 aggccagggg cgacaagcgt gccgtggcgg ggtgtctcgc ccggggcgat cgccccggtg
     3241 gagatgacgc cgctgagctt gacctcgtac tcgatcagtc cgtcgttgta gaggtaccag
     3301 aagtagccgt actcgtagtt ccccacggtg acgatcgtgg agatgaccag ccgccgcctg
     3361 cgccggacct gcaccgctcc ggtgcgaaag tcggtgtgct tccaggcgat ccctgcgtcc
     3421 tcctcgtgca tgcagacggc gttcgggatg atcaccggtg cgccgtcgtt gtcgctgacg
     3481 accccgtcga agtactggat gtgcccgacg cagtcgcagc cgagggtcag cgagttcgcg
     3541 agccagccga cgccgtactc gccctggtcg aacacgttct tgaaccggtg ggtgggggcc
     3601 gggtcgccgt acgggacgta catctcggcg agcgacgccc ggtagacgat cggccgtccg
     3661 gcgtagccga gctggtgcag caccaggccc tcacgcgggg tgaacccgat ccgcaggtgc
     3721 cacttctgcc acgtcaccgc gtgcccgtcg accgtgaagc tcgggccgtc gggctgggtg
     3781 atctcgatcg gcttgacgtc gtcacggtag gcgtcgaacg cggggacgtt ccccggctcg
     3841 aagacccacg gctcctcgta gttgcccggc tgctcgggga tcgggacgac gccgtggtcg
     3901 tcgacctcga ccacctcgcc tgcattgagg tccaccacca cgaccaagcc ctcgacgggc
     3961 cgcgcgtacc cgttctcgcc cggtgccgag cgcacccagg tgagcggtcg ggcgatccgg
     4021 cggaccgacg ggtcgtcctg ggggccggtc cagctcgagg cccacgggtc gaccatggtg
     4081 agtgagaagt cctcgatccc gcgccggcgc atggccgcct gccagcgcgg gtcggcctga
     4141 acgatcttct cgcaggacat gaactcctcc gccatgatcg gcgcctgcac gccctcgatc
     4201 gcgcggaagg acaccaccgc ccggtcgctg agcgagacga ccgcctcgta ggtggtgcgc
     4261 tcgctgcgct cgtacagcac gacgtgtgcc tcgcgcggca gcggctcgtc gccgggcgac
     4321 caggcgaaca cggcggcctt cggcggctcg tgcagcgtga cgaacacgaa ccgcgccgtc
     4381 tccgccagtc cttcggccgc ccggagcgcc tcggcggcgg cggcgacctc cgtgccgctg
     4441 agcggctcca gcggatggcc ggcgacagcg gcgggggtga tcgtgacgct cgcgtcctcg
     4501 acggcagtca tgcgtgggct ccctgcggtg tgtcggtgga atcgccgatc tcggtgacct
     4561 cgaactgacc tattcgggcg tacctgctgg ggtaccggct gcgcaagacc acggccacgc
     4621 ccattcctcc gaggaacacc gcgagtacca cccagggcag cagccggatg taggcggcgt
     4681 cgcccgctcc ggcgaggtcg tagcggttga cgacgagcag cacgcacgca cccgtcatgg
     4741 ccgcgaagcc gagcgccggc gccaccaggg tcgaccacca gcgaaaaccg tcgcgggccg
     4801 ccgtgaggaa gtatcggatg atcgcgacgg acaccagcgc ctgcacgccg aggatgccca
     4861 gcacgccgag gagcggcgac cacgtgccca gcttgagcag cgcgccctcg ttggtggggt
     4921 cgtagatcac gaaggcaagg cagtagagcg ccacgaggac cgtcaccgtc atcgaggcgg
     4981 tcaccgggct gtgccgcgtg gacgtgcggg cgagcgcact cggcagcacc ccctcacgcc
     5041 cgagagcgaa caggtaccgg gcgctggtgt tgtagaaggc catcgcgcac gcgaacgagc
     5101 ccgtcacgat gagcacctcc atgagcgccg tggcccaggc gccgacgtag cggtcggtga
     5161 gcgggtagaa cgccgaggcg atctggcccg cgtactgcga cttcaccgcc tccgccgagc
     5221 cgttcaggcc ccagccggtg acgaacacgt aggagatgag gatgtagaac aggccgaggc
     5281 cgatcaccga tccgtacgtg gcggccttcg cgatccggtg cgggtcgcgg gtttcctccg
     5341 cgtagttcgg cgccatctcg aagccgaccc acgaccagaa cgccgcgaac aacgcgactc
     5401 ccgcggcacc cgccccaaag acctggatcg ccgcggagtt gtcgaagatg ttcagcgggt
     5461 tcagcggggc gagcgagaag ccctcggcgc cgcctgcggc gaacacgccg acggccagca
     5521 ccacgagcac cagcaactcg gcgatgagcg ccacgccgag caccttggcc gtgagctcga
     5581 tatggaacac cgcgaacgcg ctcatcagcg cgagcccgat gatcatgtag acccaggcgg
     5641 gcaggtccag gccggtgaaa ctcgcgacgg tcgtggacgc gaagtagccc atcgtcccgg
     5701 tcaccgcggc ggcgaagacc atgtaacaaa gcacgatcag cagcccggaa ccgaggccca
     5761 gcacccggcc gaggccgcgg gtgacgaagg tgtagaagcc gccggccgac gtgacgcggc
     5821 gacacatggc gatgtagccg accgagaaga tcgtcagtgc gacggtcgcg acggcgaacg
     5881 cggcggggga ggcatacccg ccgccgagtg tcgccacggg tacgttgaac agcatcgcgg
     5941 cgagcggggc tgccccggtg acgatgcaga acagcacgga cgggaggccc agcgctccgt
     6001 cggcgagctg gccgggcttc ccggactcgg ctggtaaggc attggtttcg gtgggtacgg
     6061 cggtcatggc ggctcccggg ggtcgagggg ctcgggacgt cggtgtagga cctgcggtgt
     6121 cgtggacaag cctgcggagc gcacgtgtcc tcagtgtcac ctgtgagtga catgctgccc
     6181 atcacaaatt tgggccagag acatcagtgc gtagtagacg gatctcgctg cttctcccgg
     6241 gacgacgacc ccaggcgccg ccgcgccgag ccgcccggga cgatgtgtgg gtgaccagca
     6301 cgccgcttcc gacccagcag aagccgaccc agcagcactc gatccagcag cggatcgccg
     6361 acgagctcgg cgtccggccc catcaggtgg cctcggccgt cgacctgctc gacggtggcg
     6421 cgaccgtgcc gttcatcgcc cggtaccgga aggaggccac cggaaccctc gacgacgcac
     6481 agctgcgcac gctcgaggag cggctgcgct acctgcggga gctggaggag cgccgcacgg
     6541 tggtgctcga cgagatccgc tcgcagggca agctcgacga ggccctcgag gcacggatca
     6601 tggccgcgga gtcgaaggcg cgcctggagg acatctacct gcccttcaag ccgaaacgcc
     6661 gcaccaaggc gatgatcgcg cgggaggccg ggctcgagcc gctggccgac accctgctgg
     6721 ccgacccgtc ccaggacccg gcaggcgtcg ccgcagggtt cgtgaacgag caggtggccg
     6781 acgttgccgc tgcgctcgaa ggtgcccggt cgatcctcgt ggagcggttc gcggaggacg
     6841 ccgacctgat cggcggcctg cgcgagcgga tgtggacgcg cggcaggttg gtcacgaagg
     6901 tccgcgagga caaggagacc gagggggcga agttcgccga ctacttcgac ttctccgagc
     6961 cgttcaccaa gcttccctca caccggatcc tcgcggcgtt ccgcggggag aaggaggagg
     7021 tcctcgacgt tgcgctggag cccgaggacg gcacggaacc cgccgagccg ggcgccgtca
     7081 ccgactacga acgcatcatc gcgaccgcgg tcggcgtgag tgaccagggc cgccccgccg
     7141 acaagtggct ccgcgacgcc gtgcggtggg cctggcgcac ccggatcctg ctgcacctgg
     7201 gcatcgacat ccgggtgcgg ctgcgcacgg tggcagagga gggcgcgatc ggcgtcttcg
     7261 ccacgaacct gcgcgacctg ctgctcgccg cgcctgccgg cagccgggcc acgatgggcc
     7321 tggaccccgg gctgcgcacg ggggtcaagg tcgccgtggt ggacgccacc ggcaaggtcg
     7381 tgaccaccga cacgatctac ccgcacgagc cgcggcgcga ctggaagggt gccctcgcga
     7441 cgctcaccag gctggcggcc gcccacgacg tcgagctgat cgcggtcggc aacggcactg
     7501 cctcgcggga gaccgaccag ctggccaggg agctcgccgc gaagaaccca gccctgaaac
     7561 tgatcccggt gatggtcagc gaggccggtg cgtcggtgta ctcggcgtcg gcgtacgcct
     7621 cggccgagct gcccgagctg gacgtatccc tgcgcggagc ggtgtcgatc gcgcgacggc
     7681 tgcaggaccc gttggccgag ctggtgaaga tcgatccgaa gtcgatcggc gtgggccagt
     7741 accagcacga cctggccgag tcggcgctgt cccgctcgct ggacgccgtg gtcgaggact
     7801 gcgtgaacgc cgtcggggtc gacgtcaaca ccgcgtccgc gccgctgctg cgcagggtct
     7861 ccggcatcac cgagggcctg gccacgcaga tcgtgctgca ccgggacgcg aacgggccct
     7921 tccgcacccg gcgcgcgctc gccgacgtgc cccgcctcgg gcccaaggcg ttcgagcagt
     7981 gcgcgggctt cctacggatc cgcggcggcg acgacccgct cgacgtctcc ggcgtccacc
     8041 cggaggcgta cccggtggtg caccggatcc tggagcgggc gaagaccgac atcggctcgc
     8101 tgctcggcaa cgcacagaag atcaagtcgc tgcggcccca cgagttcgtc gacggcacct
     8161 tcggcctgcc gaccgtcacc gacatcctcg ccgagctgga gaagccgggg cgcgaccccc
     8221 gccccgcgtt ccgcaccgcg acgttcgccg agggcgtcca caagatctcc gacctgcagc
     8281 cggggatgct gctggagggc caggtcacca acgttgcagc gttcggcgcc ttcgtggacg
     8341 tcggcgtgca ccaggacggg ctcgtgcacg tgtcggcgat gtcgaagcag ttcgtcagcg
     8401 acccgcgcga cgtcgtcaag tccggcgacg tggtgcgcgt caaggtgctg gaggtggacg
     8461 aggcgcgcaa gcgcatctcg ctgactctgc gcctggacga cgaggtgggc gcgaagccct
     8521 cccgggggga ccagcgcggc ggcgaccaac ggcgccctga acgcgggccc cgtgaccaga
     8581 agggcggccg ggacgagaag ggcggccggg accagaaggg ccgtgatcag aagggtggcg
     8641 accagaaggg cggcgctcgc ggggatcagc gggtccgcgg cggcgaccgg cgcggcggcg
     8701 gagggcggca gggcggttct ggcggctccg gcggaggcct gggcaacagc gcgatggccg
     8761 acgccctgcg ccgtg
//
LOCUS       DASRSC010000142        15410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2831854,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000142 DASRSC010000000
VERSION     DASRSC010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 15410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15410
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2831854"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="VFE39_06785"
     CDS             <1..342
                     /locus_tag="VFE39_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6258340.1"
                     /translation="QQLRTLLGSFMFSGDQLQQPAGTLSGGEKTRLALAGLVSSAANV
                     LLLDEPTNNLDPASREQVLDALRHFAGAVILVTHDSGAVEALHPDKVIVLPDGTEDLW
                     SSEYLELVQLA"
     gene            667..876
                     /locus_tag="VFE39_06790"
     CDS             667..876
                     /locus_tag="VFE39_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003071237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6258341.1"
                     /translation="MADLKKGARITGTQRDKLATDLKKKYEKGASIRALAEQTGRSYG
                     FVHRVLSETGVPLRGRGGATRTKKK"
     gene            902..1705
                     /locus_tag="VFE39_06795"
     CDS             902..1705
                     /locus_tag="VFE39_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET6258342.1"
                     /translation="MTTAVVDPGLLERGGLALDVDGPRATISLSRPDVLNAQTPVTWD
                     AFRSIGEALPDEVWVVVVRGEGRAFSSGLDRRFFTAREIDGLPGIAGIAELSDEDADT
                     TIAGFQAGFSWLRHPARVTVAAVQGHAIGGGFQLALACDLRLVADDVQFCMAEATLGI
                     VPDLGGTHPLVQAVGYSRAVEICLTGRRVGAEEAVASGLALRAVPVGQLDAEVDAIVS
                     SLLAVPSGAARETLALLAGVADGMDPAGALAAERAAQLRRLRSLVAGEG"
     gene            1816..3753
                     /locus_tag="VFE39_06800"
     CDS             1816..3753
                     /locus_tag="VFE39_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6258343.1"
                     /translation="MRGVARGVDAPKSVKKGTIPRIWTFARPYRRWLLAFLLLTVVSA
                     TIGVLTPVLAGRVVNTIVARGEVSAAAATVLGLAGGIAALAVLEAVVGLVSRWFSSRI
                     GEGLIVDLRRAVFEHVQRMPIAFFTRTRTGALVSRLNNDVMGAQSAITSTLASVLSNA
                     VQLALALGVMISLAWQVTLLALLLLPIFVLPARRMGRTIAGLRRESADLNASMSTQMT
                     ERFSAPGATLVKLFGRPEEEARDFGERAERVRDIGVRTAMVSRVFVTALSLVSALAQA
                     LVYGLGGYLAVTGQIEPGTVVTLALLLTRLYTPMTGLANARVDVMTALVSFERVFEVL
                     DLKPMITEKQGAAPLPDGPVTVRLRDVRFGYPNAEQVSLATLEEVAVLDNRVNEEVLH
                     GVDLDVGAGQLLALVGSSGAGKSTLASLVPRLYDVDSGAVELSGIDVRDLTSAAVRGA
                     VGVVTQDGHLFHDTIRANLRYAAPEASDAEIVDAVRRARLGDLLDALTDGLDTVVGER
                     GYRLSGGERQRLTIARLLLARPRVVILDEATAHLDSESEAAVQAALAEALVGRTAIVI
                     AHRLSTIRAADEIAVVEQGRIVEHGTHEQLLARGGRYAALYRTQFADGGDEPGDEPLP
                     PGIACGIPGCTGRHREQHLVG"
     gene            3807..4130
                     /locus_tag="VFE39_06805"
     CDS             3807..4130
                     /locus_tag="VFE39_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258344.1"
                     /translation="MEAGDRRVDADRSAHHPSVSSVGGEQPGWSTSMIMICERCYAPI
                     GDGDAVVRLAHVDETRPDGSVRWRYSYVHPAGSAACGTTLPPDGQRPDTGAWNPARGI
                     DGRRA"
     gene            complement(4156..4725)
                     /locus_tag="VFE39_06810"
     CDS             complement(4156..4725)
                     /locus_tag="VFE39_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6258345.1"
                     /translation="MPRVSADQLAARRRQILDGARACFARYGYEGATVRRLEQSTGLS
                     RGAIFHHFRDKDALFFALAERDAERMTEIVAEQGLVQVMRELLTSPERIDAGWTGTFL
                     EIARRGRTDPGFRDRWRQHAEAITAATQARLERQARTGTLRDDVPVPVLARYLELVLE
                     GLVSHLSMELPAEDLAGVLDLVEGAVRRR"
     gene            4846..6171
                     /gene="egtA"
                     /locus_tag="VFE39_06815"
     CDS             4846..6171
                     /gene="egtA"
                     /locus_tag="VFE39_06815"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis glutamate--cysteine
                     ligase EgtA"
                     /protein_id="HET6258346.1"
                     /translation="MAEDVGITMISAAPDAAPPDAGPSVLRDCADAEAYVASVCFKIG
                     PPRLVGVELEWLLHRPSAPTVPPDVDALIAALGRHSPTTLDPSSSALPLPAGSDVSVE
                     PGGQIELASPPLPDLGGLLQSVRADADALHHLLATQGLRPHTRAADPLRAPVRVLDVP
                     RYRAMEQAFDRVGPHGRTAMCSTAAVQVCVDAGERGEVARRWAAVHALGPVLLAAFAN
                     SPVLHGRRTGWKSSRWASWQRADPARTAPPPVLDTTTADPAAAWARRVLETPVLCVRR
                     DGVWEVPRGVTFADWVNGALGAPPTTSDLAYHVSTLFPPVRPHGHVEVRYVDAQAGRR
                     WALPVAVLAALLADPAVTDRAREACEPAQGRWTAAARHGLADRMLARAAATVFQLACS
                     QLPAIGAPDWVVADLADMTEQQVLRGLCPADNCADDPADDPDTPEGETS"
     gene            6168..7502
                     /gene="egtB"
                     /locus_tag="VFE39_06820"
     CDS             6168..7502
                     /gene="egtB"
                     /locus_tag="VFE39_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HET6258347.1"
                     /translation="MTVDHVAATDEALRTRVADLLAASRARSTALTEAVDEADLVAQH
                     SPLMSPLVWDLAHIGSQEELWLVRDVGGREPLRPEIDGLYDAFQHSRASRVELPLLTP
                     AETRSYVAEVRDKALDVLERTPLTGRRLVERGFAFGMIVQHEQQHDETMLATHQLRAG
                     APVLHADAPPAGRPLAAREVLVPAGPFTMGTSAEPWALDNERPAHVVEVPAFVIDAAA
                     VTNGDYAEFIVAGGYDDQRWWSDAGWAHRVQAGLTAPLFWERDGSGEWYRRRFGVLER
                     VRADEPVVHVCFHEADAYARWAGKRLPTEVEWEKAARFDPATGRSRRFPWGDDDPTPE
                     RANLGQRHLQPSAAGAFPSGASLLGVHQLIGDVWEWTSSGWHPYPGFQVFPYPEYSQV
                     FFGGDYRVLRGGSFGTDPAAIRSTFRNWDHPVRRQIFAGFRCARDVSPAGVA"
     gene            7509..8267
                     /gene="egtC"
                     /locus_tag="VFE39_06825"
     CDS             7509..8267
                     /gene="egtC"
                     /locus_tag="VFE39_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtC"
                     /protein_id="HET6258348.1"
                     /translation="MCRHLAYLGPPVTLSSMVLEAPHGLLRQSYAPADMRGGGTINAD
                     GFGAGWYPEPGADAVRYRSAAPIWSDLAFPAVAAATRSGALLAAVRSATTGMPVTVTA
                     AAPFAEGRWLFSHNGVVRGWPRSVASLAAELPVTDLITLDAPTDAALLWALVRHRLRA
                     GADPAVALADVVTAVATSAPGSRLNLLLTDGTAIWATAWDHALSVRTTPGTALVASEP
                     IDSLPTWNGVPDRHLVVAYPGRHRVQDLTTTGVP"
     gene            8264..9235
                     /gene="egtD"
                     /locus_tag="VFE39_06830"
     CDS             8264..9235
                     /gene="egtD"
                     /locus_tag="VFE39_06830"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HET6258349.1"
                     /translation="MNHALLDIHLTDGDADVALRADVRAGLTAIPKQLPPKWFYDARG
                     SELFELITELPEYYPTRTERALLARSVDEIAVTSGADTLVELGSGSSAKTRLLLDAFF
                     RSGSLRRYVPQDVSEAALRLAMDALAVEYPRLALHGVVGDFTTHLDRLPRGDHRMVAF
                     LGGTIGNLLPEERAEFLRQLRAVLHPGEQLLLGTGLVIDEEVLVRAYDDSAGVTAEFN
                     RNVLRVLNRELGADFVIEAFAHRALWDAENEWIEMRLVAERAMTVTVADLGLRVQFAA
                     GEEMRTEVSAKFRPAGVRAMLEEAGLAVTRTWTDPDDRFALTLATAT"
     gene            9410..9880
                     /locus_tag="VFE39_06835"
     CDS             9410..9880
                     /locus_tag="VFE39_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6258350.1"
                     /translation="MSSGGSWRRDGVRVIPGDALDSNTPQTPGMQRAAAVTHGRVGAT
                     RLWAGTVTIHPGARTGAHHHGELESVIYVVRGLARMRWGENLEYCAEAGPGGFIFVPP
                     FVPHQEINALDDEPLECVLTRSGQEPIVVNLTIEGVAEPEHVPWTDPAHPAPRR"
     gene            complement(9941..10354)
                     /locus_tag="VFE39_06840"
     CDS             complement(9941..10354)
                     /locus_tag="VFE39_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258351.1"
                     /translation="MAADSAFTPDVTRLGCLRGVSTLTAFGRAVEIGDWQRLTTKTGT
                     GHARRLLIEAAWDHRTRYRPGVELRRRQGKAGPAAQDRGERANQRLHARWVSFDARDK
                     RPVVANTAIARELAGWCWSHYEQFTVRPSRATLDL"
     gene            complement(10321..10497)
                     /locus_tag="VFE39_06845"
     CDS             complement(10321..10497)
                     /locus_tag="VFE39_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258352.1"
                     /translation="MLLRHGIVYYGGKPWTGPHDRWLRARRELLTLPGLGARRDRLDA
                     AIAGWPPTARSPQT"
     gene            complement(10842..13670)
                     /locus_tag="VFE39_06850"
     CDS             complement(10842..13670)
                     /locus_tag="VFE39_06850"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HET6258353.1"
                     /translation="MASTDSFGARGTIQAGGAEHEIFRLSAVEGAERLPFSLKVLLEN
                     LLRTEDGANVTADHVRALAGWNPDAEPDTEIQFTPARVVMQDFTGVPCVVDLATMREA
                     VTELGGDAAKVNPLAPAELVIDHSVIIDIFGRPDAFERNVDFEYQRNKERYQFLRWGQ
                     GAFDEFKVVPPGTGIVHQVNIEHLARVVMTRNGQAYPDTLVGTDSHTTMVNGLGVLGW
                     GVGGIEAEAAMLGQPVSMLIPRVVGFKLTGEIPAGATATDVVLTITEMLRRHGVVGKF
                     VEFYGEGVGAVPLANRATIGNMSPEFGSTAAIFPIDEETLRYLKLTGRSTEQIDLVEA
                     YAKEQGLWHDPAREPVYSETLELDLSTVVPSIAGPKRPQDRIALSESKGAFRGALGDY
                     AGEPDERDPDDAVTTRSAVDEAGEESFPASDPASPSSGNGEGAPHAPVSAATGAGGRT
                     TKPTPVTLVDGSSFELDHGAVVIASITSCTNTSNPSVMLGAALLAKSAVDKGLAVKPW
                     VKTSMAPGSKVVTDYYEKAGLWPYLEKLGYHLVGYGCTTCIGNSGPLAEEISAAVNEA
                     DLAVVSVLSGNRNFEGRINPDVKMNYLASPPLVIAYALAGTMDFDFETQSLGKDPQGN
                     DVFLRDIWPSAQDVQSTIDGAISQEMFTKDYADVFAGDERWQSLQTPEGNTFAWDPES
                     TYVRKPPYFEGMQPEPSPVQDISGARVLALLGDSVTTDHISPAGSIKEDTPAGQYLRE
                     HGVEKKDFNSYGSRRGNHEVMIRGTFANIRLRNQLLDGVSGGYTRDFTQEGVPQAFIY
                     DAAQNYAAQGTPLVVLGGKEYGSGSSRDWAAKGTALLGVKAVIVESYERIHRSNLIGM
                     GVLPLQFPEGQSAASLGLDGSETFDISGVTALNEGSSPRTVHVTATKESGETVEFDAT
                     VRIDTPGEADYYRNGGIMQYVLRGMLRS"
     gene            14092..15372
                     /locus_tag="VFE39_06855"
     CDS             14092..15372
                     /locus_tag="VFE39_06855"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HET6258354.1"
                     /translation="MLVALLGGGTSAALAQPAPPPPPNPSDDELQRSRDSVSGRADEV
                     GRLTTQLAELDTRTDDLQAALAAQRETAEAALVDLQVAQDAATAAARRADAARIATAA
                     ASVAIDQARARLDEFVTATYQQGLDIGPLGLLTDSTSPEDLVARAEFNDLIARTQLAA
                     QDGLERARVDKANADSAARAALEDARVREAEARTAKAAADAAFAAAEGAAREQAEQLA
                     AVAATRADVQRRLDAAESADAGLRQQRARFDDWQRRLAREQAARERADRDAAAGRVAA
                     GTPRGGEPAVRGGSAGVQRVIDRAMSQIGVQYVWGGGTGRGPSTGIPDGLGSPLNLIG
                     FDCSGLMLYAFSGAGVSLPRVSRNQYNVGRKVPISEIRPGDMVFYQNGSAPIHHVALY
                     IGNGKMIEAPYTGASVRVVPLRTRGLLPEAARVL"
BASE COUNT         2101 a         5474 c         5508 g         2327 t
ORIGIN      
        1 cagcagctgc gcacgttgct ggggtcgttc atgttctccg gcgaccagct ccagcagcct
       61 gccggcacgc tgtcgggcgg ggagaagacc cgcctcgcgc ttgccggtct cgtctcgagc
      121 gcagccaacg tcctgctcct cgacgagccc acgaacaacc tcgatccggc cagccgcgag
      181 caggtgctcg acgcgcttcg ccacttcgcc ggggccgtca ttctggtcac ccacgactcc
      241 ggcgccgtcg aggcgctgca ccccgacaag gtgatcgtgc tgccggacgg cacggaggac
      301 ctctggtcga gtgagtatct ggagctcgtg cagttggcct gatcgggcga tttccgcgat
      361 cttcggcacc acccgatcgg gcaattcatt ccgcgagtcg tgttgacctg cggtgatgtc
      421 ctgctccgcc ctgctcctcg aacgggcccg tgaagggaac aaacaggtgg cgagggagtg
      481 acgaggaggt gagacgggtg cgtcaatgat cacccgtccg cgtgggccgt tcggcggaac
      541 cgttcaccga tcatcgagtg gatctcctgg ttacgggtac ctaccgagga cgatcattgc
      601 gcgcaccggg gggccgggaa cgaaccgcgc gtggaagttc cgcggcggat agaggaggaa
      661 aaggccgtgg ccgacctgaa gaagggtgcc cggatcaccg gtacccagcg tgacaagctc
      721 gccaccgacc tcaagaagaa gtacgagaag ggcgccagca tccgggccct cgccgagcag
      781 accgggcgct cgtacggctt cgtgcaccgg gtgctcagcg agacgggcgt gccgctgcgt
      841 gggcgtggtg gggccacgcg taccaagaag aagtaacacc gctgcgggct aacctgcccg
      901 catgacgaca gcggtggtgg accccggtct cctcgagcgc ggcgggctcg cgctcgacgt
      961 cgacgggccg cgggcgacga tcagtctctc gcgccccgac gtcctcaatg cacagacgcc
     1021 cgtcacctgg gacgcgttcc gctcgatcgg cgaggcgctt cccgacgagg tgtgggtggt
     1081 ggttgtgcgg ggcgagggcc gcgcgttctc gtcgggcctc gacaggcggt tcttcacggc
     1141 tcgtgagatc gacgggctgc cggggatcgc cgggatcgcg gagctgtccg acgaggacgc
     1201 cgacaccacc atcgccgggt tccaggccgg gttctcgtgg ctgcgccacc ccgcacgggt
     1261 caccgtcgcg gcggtgcagg gccacgcgat cggcggcggg ttccagctcg cgctggcctg
     1321 cgacctgcgc ctggtcgccg acgacgtgca gttctgcatg gcggaggcga cgctcggcat
     1381 cgttccggac ctgggcggta cccatccgct ggtgcaggcc gtcgggtact cccgcgccgt
     1441 ggagatctgc ctgaccgggc gccgggtcgg ggcggaggag gcggtggcgt cgggactcgc
     1501 actgcgtgca gtgcccgttg ggcaactgga cgctgaggtc gacgccatcg tctcctctct
     1561 cctcgccgtg ccatccgggg cggcccgcga gactctcgcc ctgctggccg gtgtcgccga
     1621 cggcatggac cctgccgggg ctctcgcggc ggagcgggcc gcgcagctgc gccgcctccg
     1681 cagcctggtg gccggagagg gctgaatctg gcgatcggga ctaaaaggag gcaaccgcgg
     1741 tgttgtcgcg ttgcgtcagg agtgtgtgca atggtgtaag cgagggggcc tccggtggat
     1801 cgggcctggg gaatgatgag gggcgtcgcc cgcggcgtcg acgcgccgaa gtcggtgaag
     1861 aaggggacga tcccgcggat ctggacgttc gcccggccct accgccgttg gctgctggcg
     1921 ttcctgctgc tcacggtcgt ctccgccacc atcggcgtgc tgacgccggt gctggccggg
     1981 cgcgtggtca acacgatcgt ggcccgcggc gaggtgtccg ccgccgccgc taccgtgctc
     2041 ggcctcgccg ggggcatcgc ggccctcgcg gtgctggagg ccgtggtcgg cctggtgtcc
     2101 cgctggttct cctcgcggat cggggaggga ttgatcgtcg acctgcgccg cgcggtcttc
     2161 gagcacgtgc agcggatgcc gatcgcgttc ttcacccgga cccgcacggg cgcgctcgtg
     2221 agcaggctca acaacgatgt gatgggcgca cagtcggcga tcacatcgac cctggccagc
     2281 gtgctgagca atgccgtcca gctggctctc gccctcggcg tgatgatcag cctggcctgg
     2341 caggtcacgc tcctggcgct gctgctgctg ccgatcttcg tactgccggc gcgccgcatg
     2401 ggccgcacga tcgccggcct gcgccgggag tcggccgacc tcaacgcgag catgagcacg
     2461 cagatgaccg agcggttctc cgcgccgggc gccacgctgg tcaagctgtt cggccgcccc
     2521 gaggaggagg cgcgtgactt cggtgagcgg gccgagcggg tgcgcgacat cggggtgcgc
     2581 acggcgatgg tctcgcgcgt cttcgtcacc gctctgtcgc tggtttcggc gctggcccag
     2641 gcactcgtct acggcctcgg tggctacctc gcggtgaccg ggcagatcga gcccggcacg
     2701 gtcgtgaccc tcgcgctgct gctgacccgg ctctacacgc cgatgaccgg cctggccaac
     2761 gcccgcgtcg acgtgatgac cgcactcgtg tcgttcgagc gggtcttcga ggtcctcgac
     2821 ctgaagccga tgatcaccga gaagcagggg gccgctccgc tgcccgatgg gccggtgacc
     2881 gtccggctgc gcgatgtccg gttcggctac ccgaatgctg aacaggtgtc gctggccacc
     2941 ctggaggagg tggccgtgct ggacaaccgg gtgaacgagg aggtgctgca cggcgtcgac
     3001 ctcgatgtcg gtgccggaca gttgcttgcg ctcgtcggat cgtcgggcgc cggcaagtcc
     3061 acgctcgcct ccctggtgcc gcggctctac gacgtcgaca gcggcgcggt cgagctgtcc
     3121 ggcatcgacg tgcgcgacct gacctctgcc gcggtgcgcg gagcggtcgg cgtcgtcacg
     3181 caggacggcc acctgttcca cgacacgatc cgcgcgaacc tgcgttatgc cgccccggag
     3241 gcgagcgacg ccgagatcgt cgatgccgtg cgccgggctc gcctcggcga cctgctcgac
     3301 gcgctcaccg acggcctcga cacggtggtg ggcgagcgcg ggtaccggct ctccggaggg
     3361 gagcggcagc ggctgacgat cgcccggctg ttgctggccc ggccccgcgt ggtgatcctc
     3421 gacgaggcca cggcgcacct ggactccgag tccgaggccg cagtccaggc cgcgctcgcc
     3481 gaggcgctcg tgggccgtac cgccatcgtc atcgcccacc ggctttccac gatccgggcc
     3541 gcggacgaga tcgcggtggt cgagcagggc cggatcgtgg agcacggcac ccacgagcag
     3601 ctgctcgcgc ggggcggccg ctacgccgcg ctctaccgca cccagttcgc agatggcggc
     3661 gacgaacccg gcgacgagcc tctgccgccc gggatcgcgt gcggcatccc agggtgcacg
     3721 gggcgccatc gagagcagca cctggtcggc tgaccggcag ggggcgtggg gaacgccacg
     3781 tctggccggc gtgctgacga gggacgatgg aagcgggtga ccggagagtc gacgccgacc
     3841 gcagcgccca tcatccctca gtttcctcgg tcggcggcga gcagccgggc tggagcacat
     3901 ccatgatcat gatctgcgag cgctgctacg cgcccatcgg cgacggcgat gccgtcgtcc
     3961 gcctggccca cgtcgacgag acccgtcccg acggcagcgt gaggtggcgg tactcctacg
     4021 ttcaccccgc gggctccgcc gcgtgcggta caacgcttcc ccctgacggt cagcggcccg
     4081 acaccggcgc gtggaacccg gcgcggggga tcgacggccg gcgcgcctga gcagcgcgca
     4141 ccggtccagc gttcgtcagc gccgccggac agcgccctcc acgaggtcga gcacgccggc
     4201 gaggtcctcg gcgggcagtt ccatggacag gtgtgagacc agcccctcca gcaccagctc
     4261 caggtagcgc gccagcaccg ggacgggcac gtcgtcgcga agcgtgccgg tccgcgcctg
     4321 ccgctcgagc cgtgcctgcg tggcggcggt gatcgcttcg gcgtgctgtc gccagcggtc
     4381 ccggaagccc gggtcggtgc gcccccgccg cgcgatctcc aggaacgtgc ccgtccagcc
     4441 ggcgtcgatg cgttcgggtg aggtcaagag ctcccgcatc acctgcacga ggccctgctc
     4501 ggccacgatc tcggtcatac gctcggcgtc ccgctcggcg agagcgaaga acagcgcgtc
     4561 cttgtcgcgg aagtggtgga agatggcccc gcgggacagg ccggtggact gctcgagcct
     4621 gcgcaccgtg gcgccctcgt agccgtaccg ggcgaagcag gcgcgggccc cgtcgaggat
     4681 ctgccggcgt cgcgcggcga gctggtcggc gctgacgcgg ggcatcagcc gaggataccg
     4741 ggaagccaga cgtacgtacg gcttgcgaaa agtcctgacg ggtcggcccc ggctgcggag
     4801 tcgagcatct gcaatcgtcg gactgcgcgg gtagcgttct cgggcgtggc tgaggacgtg
     4861 ggcattacga tgatctcggc ggcgcccgac gccgctccgc ccgacgccgg gccgtccgtc
     4921 ctgcgggact gcgccgacgc cgaggcgtat gtggcgtctg tgtgtttcaa gatcggccca
     4981 ccgcggttgg tcggcgtgga gctcgagtgg ctgctgcacc ggccatcggc tcccacggtg
     5041 ccgccggacg tcgacgcgct gattgcggcg ctcgggcggc acagcccgac caccctcgac
     5101 ccgtcctcgt ccgccctccc gctccctgcc ggttccgacg tcagcgtcga accgggtggc
     5161 cagatcgaac tcgcgagccc gcccctcccg gatctcggag ggttgctgca gtccgtccgc
     5221 gccgacgccg acgcgctgca ccacctgctc gccacccagg ggttacgccc ccacacccgt
     5281 gcggccgacc cgctccgtgc accggtccgg gtgctcgacg ttccgcggta ccgggccatg
     5341 gagcaggcgt tcgaccgcgt ggggccccac ggccgcaccg cgatgtgctc caccgccgcc
     5401 gtgcaggtct gcgtcgacgc cggcgagcgg ggggaggtcg cccgccgctg ggccgcggtc
     5461 catgccctcg gcccggtgct gctggccgcc ttcgccaact ccccggtgct gcacgggagg
     5521 cgcacgggct ggaagtcgtc gcgctgggcc agctggcagc gtgccgaccc ggcccgtacg
     5581 gcaccaccgc cggtactcga caccaccacc gccgatccgg ccgctgcctg ggcccgtcgc
     5641 gtgctggaga cccccgtgct ctgcgtgcgc cgcgacggtg tctgggaggt gccccgcggg
     5701 gtcacgttcg ccgactgggt caacggcgca ctgggagcgc cgccgaccac ctccgacctc
     5761 gcctaccacg tgtccacgct gttcccgccc gtccgtccgc acgggcacgt cgaggtgcgc
     5821 tacgtcgacg cccaggccgg gcgtcggtgg gcgctgccgg tcgcggtgct ggccgcgctg
     5881 ctggccgacc cggccgtcac cgacagggcc cgcgaggcgt gcgagcctgc gcagggtcgg
     5941 tggaccgcag cggccaggca cgggctcgcc gatcggatgc tcgcccgggc cgcggccacc
     6001 gtcttccagc tcgcctgctc acagctgccc gccatcggcg cgcccgactg ggtcgtcgcc
     6061 gacctggccg acatgaccga gcagcaggtg ctgcgcggac tgtgccctgc cgacaactgt
     6121 gccgacgacc cggccgacga tccggatact cccgagggag agacgtcatg accgttgacc
     6181 acgtcgcggc aaccgacgag gccctgcgca cccgcgtggc cgacctgctc gcagcgtccc
     6241 gtgcgcgcag caccgccctc accgaggccg tcgacgaggc cgacctcgtg gctcagcatt
     6301 cgccgctgat gtccccgctg gtctgggacc tcgcacacat cggcagccag gaggagctct
     6361 ggctggtccg cgacgtcggc ggccgcgaac cgctccgccc ggagatcgac gggctctacg
     6421 acgcgttcca gcactcccgc gccagccggg tcgagctgcc gctgctcaca ccggccgaga
     6481 cacgcagcta cgtggcggag gtccgggaca aggccctcga cgtcctcgag cgcaccccgc
     6541 tcaccggccg caggctcgtc gagcgcggct tcgccttcgg aatgatcgtg cagcacgagc
     6601 agcagcacga cgagaccatg ctcgccaccc accagctccg cgcgggtgct ccggtgctgc
     6661 acgccgacgc tccgcccgcc gggcgcccgc tcgccgcccg cgaggtgctc gtgccggccg
     6721 gcccgttcac catgggcacg tccgcggagc cgtgggcgct cgacaacgag cggcccgcac
     6781 acgtggtgga ggtcccggcg ttcgtcatcg acgccgcagc cgtcaccaac ggggactacg
     6841 ccgagttcat cgtggcgggc ggctacgacg accagcgctg gtggagcgat gccggctggg
     6901 cacaccgggt gcaggccggg ctcacggcgc cgcttttctg ggagcgggat ggctccggcg
     6961 agtggtaccg gcgccggttc ggtgtgctcg agcgcgtgcg cgccgacgag ccggtcgtcc
     7021 acgtctgctt ccacgaggcc gacgcgtacg cgcggtgggc cggcaagcgg ctgcccacgg
     7081 aggtggagtg ggagaaggcc gcccggttcg acccggctac cgggcgctcc cggcgtttcc
     7141 cctggggcga cgacgacccc acacctgaac gcgccaacct cgggcaacgt cacctgcagc
     7201 cgtcggcagc aggagccttc ccaagcgggg cctcgctgct cggcgtgcac cagctcatcg
     7261 gtgacgtgtg ggagtggacg agctcgggct ggcacccgta cccgggcttc caggtcttcc
     7321 cctatcccga gtactcgcag gtgttcttcg gcggcgacta ccgcgtgctg cgcggcggct
     7381 cgttcggcac cgacccggcg gcgatccgga gcacgttccg caactgggac caccccgtgc
     7441 gtcggcagat cttcgccggc ttccggtgtg cgcgggacgt gtcccctgcc ggggtggcgt
     7501 agcggcgggt gtgccgtcat ctggcctatc tcggcccacc tgtgaccctg tcgtcgatgg
     7561 ttctcgaggc gccgcacggg ctgctgcggc agtcctacgc gcctgcggac atgcgcgggg
     7621 gcggcacgat caacgccgac ggcttcggcg cgggctggta tcccgaaccc ggagccgacg
     7681 ccgtgcgtta ccgcagcgcc gccccgatct ggtccgacct ggcctttcct gccgtcgccg
     7741 cggccacccg gagcggcgcg ctgctggctg ccgtccgctc ggccaccacg gggatgcccg
     7801 tcacggtcac ggcggctgcc ccgttcgccg agggccggtg gctgttcagc cacaacggcg
     7861 tcgtccgcgg ctggcctcgc tcggtcgcga gcctcgccgc cgagctgccc gtcaccgacc
     7921 tgatcaccct cgacgcgccc accgacgccg ccctgctctg ggcacttgtc cgccatcggc
     7981 tgcgtgcggg cgccgacccc gcggtggccc tcgccgacgt ggtcaccgcg gtggccacat
     8041 cggccccggg atcgcggctg aacctgctgc tcaccgacgg caccgcgatc tgggccacgg
     8101 cgtgggacca cgcgctgtcg gtgcgcacca cccccggcac cgccctcgtc gcgtccgaac
     8161 cgatcgacag tctccccacc tggaacgggg tgcccgaccg gcacctcgtg gtggcctacc
     8221 cgggccgcca ccgcgtccaa gacctcacga caacgggagt cccatgaacc acgcgctgct
     8281 cgacatccac ctgaccgacg gcgatgccga tgtagccctg cgcgccgacg tgcgcgccgg
     8341 tctgaccgcg attcccaaac agctgccccc gaagtggttc tacgatgccc ggggcagcga
     8401 gctgttcgag ctgatcaccg agctgccgga gtactacccg acgcgcacgg agcgggcgtt
     8461 gctggcacgc agcgtggacg agatcgcggt cacgtcgggg gcggacacgc tcgtcgagct
     8521 cggttcggga tcgtcggcga agacgcggct cctgctcgac gcgttcttcc gcagcggctc
     8581 cctgcgccgg tacgtgccgc aggacgtcag cgaggcggcg ctgcggctgg cgatggacgc
     8641 tttggccgtg gagtacccga ggctcgcact gcacggcgtg gtcggcgact tcaccaccca
     8701 cctggaccgg ctaccgcgcg gtgaccaccg gatggtcgcg ttcctcggcg gcacgatcgg
     8761 caacctgctg cccgaggagc gcgccgagtt cctgcggcag ctccgtgcgg tgctgcaccc
     8821 tggggagcaa ctgctgctgg gcaccgggct cgtgatcgac gaggaggtgc tcgtgcgcgc
     8881 ctacgacgac tcggcaggcg tcacagccga gttcaaccgc aacgtgctgc gggtgctcaa
     8941 ccgggagctg ggagccgact tcgtaatcga ggcgttcgcc caccgtgccc tgtgggacgc
     9001 ggagaacgaa tggatcgaga tgcggttggt cgccgagcgc gcgatgaccg tcacggtcgc
     9061 cgacctcggc ctgcgggtgc agttcgcggc gggggaggag atgcgcactg aggtctcggc
     9121 caagttccgg ccggccggcg tgcgcgcgat gctcgaggag gcgggtctcg ccgtcacccg
     9181 cacctggacc gacccggacg accgcttcgc cctgaccttg gccaccgcca cctgaccccg
     9241 cgagtcgccg gttttccgcc cgcgagtcgc ccgttcccgg cccgcgagtc gccggttttc
     9301 cgcctgcgag tcggccgacc tcgttcgagg cgctgtgatt cggtgtgagg gggcaccggc
     9361 acggttccgt gcggcccgac ggcgtcggac atcgggttag cctcgtctga tgagcagtgg
     9421 cgggtcgtgg cggcgtgacg gagtccgggt cattcctggc gacgcgttgg actccaacac
     9481 cccgcagact ccgggcatgc agcgcgccgc ggccgtcacg cacggcagag tgggcgcgac
     9541 gaggctgtgg gccggcacgg tcaccatcca ccccggtgcc cgaaccggcg cccaccacca
     9601 cggcgagctg gagagcgtca tctacgtggt gcgcggactg gcgcgcatgc ggtggggcga
     9661 gaatctcgag tactgcgcgg aggcgggtcc cggtgggttc atcttcgtgc cgcccttcgt
     9721 cccgcaccag gagatcaacg cactcgacga cgagcctctc gagtgcgtgc tcacccgcag
     9781 cggccaggag ccgatcgtcg tgaacctcac tatcgagggc gtggctgagc ccgagcatgt
     9841 gccgtggacc gaccccgcgc atcccgcacc gcggcgctga gcggggcggg gcgggtgcgt
     9901 atcgcgggac ggtgcttccg gctcgagcgt ttgcgcgggt ctagagatcg agcgtggccc
     9961 tgctcggtcg aacagtgaac tgctcatagt gtgaccagca ccaaccggcg agctcgcggg
    10021 cgatggcggt gttggccacc accggccgct tgtcccgggc gtcgaaggac acccagcggg
    10081 cgtgcaggcg ctggttggcc cgttcaccgc ggtcctgggc cgccgggccg gccttgccct
    10141 ggcggcggcg cagctcgaca ccaggccggt agcgcgtgcg gtgatcccag gccgcctcga
    10201 tcagcaaccg gcgcgcgtgt ccagtaccgg tcttggtggt caaccgctgc cagtccccga
    10261 tctcgaccgc gcgcccgaac gcggtcaacg tcgatacccc acgcagacag cccagccggg
    10321 tcacgtctgg ggtgaacgcg ctgtcggcgg ccatcccgcg atcgcggcgt cgagccggtc
    10381 ccgccgcgcc cccaaaccag gcagcgtcaa caactcacgc cgagcgcgca gccagcggtc
    10441 gtgcggaccg gtccacggct tgccgccgta gtagacgatg ccgtggcgca gcagcacgat
    10501 tccgctccac tggggcctcc cacatcttgt ggccctaccg gccaggaccc actcctgccc
    10561 agcaacccac gttcacatgt ggagaggccc caacccggaa ctccgtaccg tctagatgtt
    10621 tctccacctc gggaatcgcg cgcggcgact caggcaggcg gataccgaga atcgtgtact
    10681 caattccacg acgcactgtg cggcctcgca ggtgcggcct ctgatgtcaa tggatcattg
    10741 acgccagctt tccgtcaatc tgtcgacgac aacgatggcc gccgcaatga cgacggcggc
    10801 gagccgttaa ccggttcgcc gccgtcgagg gggagtggag ctcagctgcg gagcatcccg
    10861 cgcaggacgt actgcatgat cccgccgttg cggtagtagt ccgcctcgcc gggggtgtcg
    10921 atccggacgg tcgcgtcgaa ctcgacggtc tcgcccgatt ccttcgtcgc tgtcacgtgc
    10981 acggtgcgcg ggctcgagcc ctcgttgagc gccgtgacgc cgctgatgtc gaacgtctcg
    11041 ctgccgtcca ggccgaggga cgcggccgac tgaccctccg ggaactgcag cggcagcacg
    11101 cccatgccga tcaggttcga gcggtggatc cgctcgtagc tctccacgat cacggccttg
    11161 acgcccagga gcgcggtgcc cttggccgcc cagtcgcgcg acgagccgga gccgtactcc
    11221 ttgccgccga gcaccaccag cggagtgccc tgcgcggcgt agttctgcgc cgcgtcgtag
    11281 atgaacgcct gcggtacgcc ctcctgtgtg aagtcgcggg tgtagccgcc cgagacgccg
    11341 tcgaggagct ggttgcgcag ccggatgttg gcgaacgtgc cgcggatcat cacctcgtgg
    11401 ttgccgcggc gggagccgta ggagttgaag tccttcttct cgaccccgtg ctcacgcagg
    11461 tactgcccgg cgggagtgtc ctccttgatc gagcccgcgg gggagatgtg gtcggtggtg
    11521 accgagtcgc ccagcagcgc cagcacccgc gccccggaga tgtcctgcac cggcgacggc
    11581 tcgggctgca tgccctcgaa gtacgggggc ttgcggacgt aggtcgactc cggatcccac
    11641 gcgaacgtgt tgccctcggg cgtctgcagc gactgccagc gctcgtcgcc cgcgaagacg
    11701 tcggcgtagt ccttcgtgaa catctcctgg ctgatcgcac cgtcgatcgt ggactgcacg
    11761 tcctgggcag acggccagat gtcgcgcagg aagacatcgt tcccctgcgg gtccttcccg
    11821 agcgactggg tctcgaagtc gaagtccatc gtgccggcca gcgcgtaggc gatcaccagc
    11881 ggcggcgacg ccaggtagtt catcttgacg tcggggttga tccggccctc gaagttgcga
    11941 ttgcccgaga gcaccgagac cacggcgagg tcggcctcgt tgaccgcggc ggagatctcc
    12001 tcggcgagcg gccccgagtt gccgatgcac gtggtgcagc cgtagccgac caggtgatat
    12061 ccgagcttct ccaggtacgg ccagaggccg gccttctcgt agtagtcggt gaccaccttc
    12121 gagcccggtg ccatcgacgt cttcacccac ggcttgaccg ccaggccctt gtcgacggcg
    12181 ctcttggcca ggagcgccgc gccgagcatc accgacgggt tcgaggtgtt ggtgcaggag
    12241 gtgatcgacg cgatgacgac cgcgccgtgg tcgagctcga acgacgaacc gtcgaccagc
    12301 gtcaccggcg tcggcttggt cgtgcggccg cccgcgcccg tggccgccga caccggggcg
    12361 tgcggcgcgc cttcgccgtt gccgctgctg ggcgaggccg ggtcgctggc cgggaagctc
    12421 tcctcgccgg cctcgtccac ggcggagcgg gtggtgactg cgtcgtcggg atcacgctcg
    12481 tcgggctcgc cggcgtagtc gcccagcgcg ccgcggaacg cgcccttcga ctccgacagg
    12541 gcgatgcggt cctgcgggcg cttcggcccg gcgatcgacg ggacgaccgt cgacaggtcg
    12601 agctcgaggg tctcggagta gaccggctcg cgggccgggt cgtgccagag gccctgctcc
    12661 ttggcgtagg cctcgacgag gtcgatctgc tccgtggagc ggccggtgag cttcaggtag
    12721 cggagcgtct cctcgtcgat cgggaagatc gccgcggtgg agccgaactc cgggctcatg
    12781 ttgccgatcg tggctcggtt ggccagcggc acagcgccga cgccctcgcc gtagaactcc
    12841 acgaacttgc ccaccacccc gtggcggcgc agcatctcgg tgatcgtcag caccacgtcg
    12901 gtggcggtgg caccggccgg gatctcgccg gtgagcttga agcccacgac ccgcgggatg
    12961 agcatcgaga cgggctggcc cagcatggcg gcctcggcct cgatgccgcc gaccccccag
    13021 ccgagcacgc ccaggccgtt gaccatcgtg gtgtgcgagt cggtgccgac gagcgtgtcg
    13081 gggtaggcct ggccgttacg ggtcatcacc acgcgcgcaa ggtgctcgat gttgacctgg
    13141 tgcacgatgc cggtgcccgg cgggacgacc ttgaactcgt cgaacgcgcc ctggccccag
    13201 cgcaggaact ggtagcgctc cttgttgcgc tggtactcga agtcgacgtt gcgctcgaag
    13261 gcgtccgggc ggccgaagat gtcgatgatc accgagtggt cgatcaccag ttctgccggg
    13321 gccaacgggt tgaccttggc ggcgtcaccg ccgagctcgg tgacagcctc gcgcatcgtc
    13381 gccaggtcga cgacgcaggg gacaccggtg aagtcctgca tgaccacgcg ggccggcgtg
    13441 aactgaatct cggtgtccgg ctcggcgtcg gggttccagc ccgccagcgc gcgcacgtgg
    13501 tcggcggtga cgttggcccc gtcttcggtg cgcagcaggt tctccagcag caccttgagg
    13561 ctgaagggaa ggcgctcggc gccctcgacg gccgacagcc ggaagatctc gtgctcggcc
    13621 ccgcctgcct ggatcgttcc tctcgctccg aagctgtccg tgctggccac gtcggtgtcc
    13681 tcctggcgat ggtgggcgct ggatttctct agagagatgc gattttccgg agtctggcgc
    13741 accgacgcgt cctcggacgc agcggaccgg ctctccccaa acagtacgct tgtcctgcta
    13801 cacggtcgag ccgggggagg caggaaaact acgcccggcc gggtgtcccc tcccccgagc
    13861 gccgtgtggc cgcgtccgac ccttcgtccc ccatgtggtg gatgttcgtc cctgatacgc
    13921 gtgtgcctac cggagttgcg gctgtccgaa tggcagcatg cgggcgcttg tggagatcaa
    13981 cgacgggacg gaggcgcctt cgtgcggtat gggtccgcgc tacctccacg ctctccgcga
    14041 gatcatggcc gccggcttcc ggcctggcga cgacaggcgc tcgtcctcac cgtgctggtc
    14101 gcgttgctcg gcggcggcac gtccgccgcg ctggcccagc ccgccccacc gccgccgccg
    14161 aacccgagtg atgacgaact gcagcgcagc cgcgactccg tcagcgggcg ggcggacgag
    14221 gtcgggcggc tcaccacgca gctcgcggag ctcgacaccc ggaccgacga cctgcaggcc
    14281 gcgctggccg ctcagcggga gacggccgag gcagcgctcg tcgacctgca ggtcgcacag
    14341 gacgcggcca ccgcggccgc acgacgggcg gacgcagcgc gcatcgcgac cgcggctgcc
    14401 tcggtcgcga tcgaccaggc ccgagcccgt ctcgacgagt tcgtcaccgc cacctaccag
    14461 cagggcctcg acatcggccc gctcgggctg ctcaccgact ccacgagtcc cgaggacctc
    14521 gtcgcgcggg ccgagttcaa cgacctgatc gcccggaccc agctagcggc acaggacggg
    14581 ctggagcgtg cccgcgtcga caaggccaac gccgactcag ccgccagggc ggcgctcgag
    14641 gacgcgcgtg tccgcgaggc cgaggcgcga acggcgaaag cggcggcgga tgccgcgttc
    14701 gccgcggccg agggcgccgc acgcgagcag gccgagcagc tcgcggccgt cgcggcgaca
    14761 cgggccgacg tgcagcgcag gcttgacgct gcggagtcgg ccgacgccgg actgcgtcag
    14821 caacgcgccc ggttcgacga ctggcagcgc cggctggcgc gggagcaggc cgctcgggaa
    14881 cgcgccgacc gggacgctgc cgcgggcagg gttgcggcgg gcacgccgag aggtggagag
    14941 cccgccgttc gcggtggcag cgcgggggtg cagcgggtga tcgaccgggc gatgtcgcag
    15001 atcggggtgc agtacgtgtg gggcggcggc accgggcgtg gcccgagcac cggaattccc
    15061 gacggcttgg gttcaccgct caacctgatc ggcttcgact gctcgggcct gatgctctac
    15121 gcgttcagcg gggcaggggt gtcgctgccg cgggtgagcc gtaaccagta caacgtaggc
    15181 cgcaaggtcc cgatctcgga gatccgtccg ggcgacatgg tgttctacca gaacggctcg
    15241 gcgccgattc accacgtcgc tctgtacatc ggcaacggga agatgatcga agccccttat
    15301 acgggggcga gcgtccgtgt cgtgcccttg cgcaccaggg ggctgctgcc ggaggccgcc
    15361 cgcgtgctct gagcgcggtg gctctgatca ggggtgccgc gacatgggtg
//
LOCUS       DASRSC010000143         4626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_702260,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000143 DASRSC010000000
VERSION     DASRSC010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702260"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VFE39_06860"
     CDS             <1..922
                     /locus_tag="VFE39_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET6258355.1"
                     /translation="ADLLAAVPALADSVELETRTLAAAPGASLGFTDVFAALDWARAA
                     VDNGAVGAVLVQGTDTLEETSYLLELHWDREQPLVLTGAMRSPRQAGADGPANILAAS
                     AVATAPSSRGLGVLVTINDEVHSATRIRKADTTALDAFTSAPFGPLARIVEGKVTYAN
                     RPRRWAALPRPTTGQVPRVALLEASLGDDGTLLRMAYDAGYDGVVISAFGAGHVSADV
                     AEVVSAAVERGPVLLASRTGRGSVLTHTYGFAGSEEDLLSRGVISAGWLDGRKARILL
                     WSLLAAGSTPARIREVVTVRGGRPGGPDQN"
     gene            1013..1633
                     /locus_tag="VFE39_06865"
     CDS             1013..1633
                     /locus_tag="VFE39_06865"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6258356.1"
                     /translation="MLGTLRSAILGGTLAGGTRLVQSELASQLGVSTTPVREALRDMA
                     TEGLVVFDPHRGAIVRPLDIAEVREIYELRITLEPLMVRRVVNHITDEQLDRADGLAA
                     RMRTETNLSTWVELNRDFHAVFGEIGEGSRLAGMLASLRDSASAYVSLSLDARPEQVP
                     QANHEHDELVKLYRERDADGAIELTLQHLRSTLVAIEVASERGGIR"
     gene            complement(1667..2818)
                     /locus_tag="VFE39_06870"
     CDS             complement(1667..2818)
                     /locus_tag="VFE39_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6258357.1"
                     /translation="MHTLDVEESAIVDTVRDFVDRDVRPVVRELEHANTYPEKLIDQM
                     KELGIFGLAIPEPYGDVRVSTPCYAMVTEELSRGWMSLAGAMGGHTVVAKLLLDHGTE
                     EQKRRYLPRMATGELRATMALTEPGGGSDLQAMRTVARRDGDSYVINGGKTWITNARR
                     AGVVALLCKTDPQAQPTHSGISVLLVEKVPGFTVSADLPKLGYKGVESCELSFDDCRV
                     PTGALLGGVEGRGFAQMMRGLEIGRIQVAARATGVARAAFDDALRYAQERESFGKPIW
                     QHQSVGNLLADMGTKITAARQLLLHAAQRYDSAERADLEAGMAKLFCSETAMQVAIDA
                     IRVHGGYGYSTEFDVERYFRDAPLMIVGEGTNEIQRNVIVRQLVSRGGL"
     gene            complement(2918..3799)
                     /locus_tag="VFE39_06875"
     CDS             complement(2918..3799)
                     /locus_tag="VFE39_06875"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6258358.1"
                     /translation="MSLVQDQTAPDRVPTVDAHHHLWLAEEQEQPWRIGAHPALARDF
                     EPADLGPRLDEAGIEATVLVESADTSAENDRLARYAAGFSRVAGVVGWLPLEDPDRAR
                     AELDRADRDRWCGVRCVVGRAPLDWLGTSATVRLMRDLAAAGLAWDVVPVTDAQARAV
                     VELAREVPQLRIVVDHLARPPLDTGDRESWASRLGTLAACENVALKVSVGMDVLTAWP
                     RWEKADLAPYVGRAVDLFGPRRLMLASNWPVVTLRAEYGTAIRDLDGAVAGCGLSPAE
                     LSDVRGGTAGRWYALSF"
     gene            3980..>4626
                     /locus_tag="VFE39_06880"
     CDS             3980..>4626
                     /locus_tag="VFE39_06880"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6258359.1"
                     /translation="MTSGARSGMNADRAAEVRIDPPSLVALAADSIRKMILSGKLEPG
                     ERLIEERLTEHLGISRPPLREALRLLQNEGLVETRPRYGSTVATLTDQDVFEILTLRS
                     GLERLAVELGVPVREPDRLVACHEALARMEQNARDADRGSLVENGYAFHASIVALAGH
                     RRLSDMYRSVQQQQLLCMARNLGTRECYHENLVDHVRRHRHLLELIEAGDPQAVLA"
BASE COUNT          679 a         1714 c         1590 g          643 t
ORIGIN      
        1 cgccgacctg ctggcggcgg tgcctgcgct cgcggactcg gtcgagctgg aaacgcggac
       61 cctggccgcc gcgcctggcg catcgctcgg tttcaccgac gtgttcgcgg ccctggactg
      121 ggcccgagcc gccgtcgaca acggggccgt cggagcggtg ctcgtccagg gcaccgacac
      181 cctggaggag acctcctacc tgctcgaact gcactgggac cgggaacaac cgctcgtgct
      241 caccggagcc atgcgctcgc cccggcaggc aggtgccgac ggtccggcga atatcctcgc
      301 cgcatcggcg gtggcgaccg ccccgtcctc ccgaggcctc ggcgtgctgg tcaccatcaa
      361 cgacgaggtg cactccgcga cgcgcatccg caaggccgat accaccgcgc tggacgcctt
      421 cacctcagcg ccgttcggac cgctcgcccg catcgtcgag ggaaaggtca catatgcgaa
      481 ccggccccgc cgctgggcgg ccctccctcg acccaccacc gggcaggtcc cgcgggtggc
      541 gctgctggag gcgagcctcg gcgacgacgg cacgctgctg cgcatggcct acgacgcggg
      601 atacgacgga gtcgtgatct ccgcgttcgg cgcaggccat gtgtccgccg acgtcgccga
      661 ggtcgtgtcc gcggcggtcg agcgcgggcc ggtgctcctg gccagccgca ccgggcgcgg
      721 ctccgtgctc acccacacct acgggttcgc cggctccgag gaggatctgc tctcgcgcgg
      781 ggtgatatcg gccgggtggc tggacggacg caaggcgcgc atcctgctgt ggtcgctact
      841 ggcggccggc agcacgcccg cccggattcg cgaggtagtg acggtacggg gtggacgtcc
      901 cggtggtccg gatcagaact agactccacg tcggacaacc gcacaggtgg aggcatcgtt
      961 gactctcagc aacctcaggc cggtcggtcg gtccaggacc gcacatgagt acgtgctcgg
     1021 gacgctgcgc tcggcgatcc tgggtggcac ccttgccggc ggcacccggc tcgtgcagtc
     1081 ggagctcgcc agccagctcg gcgtgagcac cacaccggtg cgcgaggcac tgcgggacat
     1141 ggccaccgaa gggctggtcg tcttcgaccc gcaccgcggc gcgatcgtgc gccccctcga
     1201 catcgccgag gtccgggaga tctacgagct gcgcatcacg ctcgaaccgc tgatggtccg
     1261 ccgcgtcgtc aatcacatca ccgacgagca gctcgaccgg gccgacgggc tcgccgcgcg
     1321 catgcgcacc gagacgaacc tgtcgacctg ggtcgaactg aaccgcgact tccacgccgt
     1381 gttcggtgag atcggtgagg gcagcaggct ggccgggatg ctggcgagcc tgcgcgacag
     1441 cgcgtcggcc tatgtcagct tgtccctgga cgcccgaccg gagcaggtgc cccaggccaa
     1501 ccacgaacac gacgagctgg tcaagctcta ccgcgagcgg gacgccgacg gggcaatcga
     1561 gctgacactg cagcacctac ggtccaccct cgtcgcgatc gaggtggcgt ccgagcgcgg
     1621 cggcatccgc tgacgcgcgg cgggcgctga cgcgcggcgg gcgagctcag agcccgccgc
     1681 gcgacaccag ctggcgcacg atcacgttcc gctggatctc gttggtgccc tcacccacga
     1741 tcatcagcgg ggcgtcccgg aagtagcgct cgacgtcgaa ctcggtggag tagccgtagc
     1801 cgccgtgcac ccggatcgcg tcgatggcca cctgcattgc ggtctccgag cagaacagct
     1861 tggccatccc ggcctcgagg tcggcccgct cggccgagtc gtagcgctgc gccgcatgca
     1921 ggagcagctg gcgggcggcc gtgatcttcg tacccatgtc ggcgaggagg ttccccacgg
     1981 actggtgctg ccagatgggc ttgccgaaac tttcccgctc ctgggcgtag cgcagcgcat
     2041 cgtcgaacgc ggcgcgggcg acgccggtgg cccgggcggc cacctggatc cggccgatct
     2101 ccaacccgcg catcatctgc gcgaagccgc ggccctccac cccgcccagg agcgcgcccg
     2161 tcggcacccg gcagtcgtcg aaggacagct cgcagctctc cacgcccttg taccccagct
     2221 tgggcaggtc cgcggagacc gtgaaccccg ggaccttctc caccagcagc acgctgatcc
     2281 cgctgtgggt gggttgcgcc tgcgggtcgg tcttgcacag cagcgcgacg acaccggctc
     2341 ggcgggcgtt ggtgatccag gtcttgccgc cgttgatcac gtaggagtcg ccgtcgcgcc
     2401 gcgcgaccgt gcgcatggcc tgcaggtccg acccgccgcc cggctcggtg agcgccatcg
     2461 tcgcgcgcag ctcaccggtg gccatccggg gcaggtagcg ccgcttctgc tcctccgtgc
     2521 cgtggtcgag cagcagcttt gccaccacgg tgtgcccgcc catcgcaccg gcaaggctca
     2581 tccagccccg cgacagctcc tcggtgacca tcgcgtagca cggcgtcgac acccgcacgt
     2641 cgccgtaggg ctcggggatg gccaacccga agatccccag ctccttcatc tggtcgatca
     2701 gcttctccgg gtaggtgttg gcgtgctcca gctcgcgcac caccgggcgg acgtcgcgat
     2761 cgacgaagtc gcggaccgtg tcgacgatcg ccgactcctc gacgtcgagt gtgtgcatcc
     2821 tgcgcgtcct ccccgctcgc cggcctctcc cgaccagaca accacgatcg gtccgcgcgc
     2881 gggcctgccg ggcgcgccga cggccgccta tcggacatca gaacgacagg gcgtaccagc
     2941 ggcccgcggt gccaccccgg acgtcggaga gctcggccgg ggacaggccg cagcctgcaa
     3001 cggcgccatc gaggtcgcgt atcgctgttc cgtactcggc acgcagcgtg acgaccggcc
     3061 agttgctggc cagcatcagc cggcgcggcc cgaacaggtc cacggcgcgc cccacgtacg
     3121 gggccaggtc ggccttttcc caccgcggcc atgcggtcag cacgtccatg ccgaccgaca
     3181 ccttcagcgc cacgttctcg caggcggcga gcgtgcccag ccggctcgcc cagctctcgc
     3241 gatcaccggt gtccagcggc ggccgtgcca ggtggtcgac gacgattcgc agctggggca
     3301 cctcccgcgc cagctcgacc accgccctgg cctgcgcgtc ggtgaccggg acgacgtccc
     3361 acgcgaggcc tgcggccgcc aggtcgcgca tgagccggac cgtggccgac gtacccaacc
     3421 agtcgagcgg ggcgcgcccg accacgcagc gcaccccgca ccaccggtcc cggtcggccc
     3481 tgtcgagctc cgcgcgcgcc cggtccggat cctcgagggg caaccacccg acgacgccgg
     3541 ccacccggga gaatcccgcg gcgtagcggg ccagccggtc gttctccgcc gacgtgtcgg
     3601 cggactcgac cagcacggtg gcctcgatcc ccgcctcgtc gagccggggg ccgaggtcgg
     3661 cgggctcgaa gtcgcgggcg agcgcgggat gggcgccgat ccgccacggc tgctcctgct
     3721 cctcggccag ccacaagtgg tggtgcgcgt cgacggtcgg gacgcggtcg ggagcggtct
     3781 ggtcctgcac gagactcatc gatggccttt cacgaaggcg gtcgtcacat acgaagggac
     3841 ccgaacgggt gcctcgcctc cccttcccgt cggatccgac tattgtctac acaatctacg
     3901 cagcgcacga cagggcgtgg gaaggtgctc tcatgacagg cgaccgagcc gacccctgtg
     3961 agacgcaagg gggtccgtgg tgacctccgg cgcccgcagc ggcatgaacg ccgacagggc
     4021 ggccgaggtg cggatcgatc cgccgagcct ggtcgcgctg gcggccgact ccatccgcaa
     4081 gatgatcctg tccggcaagc tggagccggg cgaacggctc atcgaggagc ggctgaccga
     4141 gcacctgggc atcagccgcc cgcccctgcg ggaggcgctg cggctgctgc agaacgaggg
     4201 cctcgtcgag accaggccgc gctacgggtc gaccgtcgcc accctgaccg accaggacgt
     4261 gttcgagatc ctcaccctgc gcagcggcct cgagcggctg gccgtggagc tcggcgtgcc
     4321 ggtgcgcgag ccggaccggc tcgtggcctg ccacgaggca ctggcccgga tggagcagaa
     4381 cgcgcgcgac gccgaccgcg gatcactggt ggagaacggc tacgccttcc acgcctcgat
     4441 cgtggcgctg gccgggcacc gccgcctcag cgacatgtac cgctccgtgc agcagcagca
     4501 gctgctctgc atggcccgca acctcggaac ccgcgagtgc taccacgaga acctcgtgga
     4561 ccacgtgcgg cgccaccgcc acctgctcga gctgatcgag gcaggcgatc cgcaggcggt
     4621 actggc
//
LOCUS       DASRSC010000144        11672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4470632,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000144 DASRSC010000000
VERSION     DASRSC010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11672
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4470632"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VFE39_06885"
     CDS             complement(<1..342)
                     /locus_tag="VFE39_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phospho-D-threonate 3-dehydrogenase"
                     /protein_id="HET6258360.1"
                     /translation="MTPLLGLTVGDPAGIGPEVTLKAAVDPRVDVARLVAIGSLRALR
                     SAAVVVPDAPALREVSTPGEARFDPGTLDVLPVPSDVAVPVGKVSAEAGQIAFEGVER
                     AVALALAGEIDG"
     gene            complement(339..1391)
                     /locus_tag="VFE39_06890"
     CDS             complement(339..1391)
                     /locus_tag="VFE39_06890"
                     /inference="COORDINATES: protein motif:HMM:NF028352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HET6258361.1"
                     /translation="MAVVADDLTGAADAAAPFAARGLAVSVALSDASAPDAEVLALLT
                     DNRWRPASEAAERMRAAVSRVRGWGAEMLFVKVDSTMRGRVHADVAVALDAWRGSGAV
                     ATPAFPGQGRTVRGGALVVHGETTIEQVADHFPPGVLVVDAETDDDLVEIARRVVDTG
                     SVAVGSGGLSRALAGVLVPAPGPTPGPRTAATGVLVVVGTPHATTGAQVALLLAGGAE
                     LVVVGTTSSSVDTAAAALRAGRRVVLTCEVTGDVEPDSPRAAALAEDLARVVTAVLGA
                     APSAGLVLTGGATALAVVRALGAVEFRLTSEVAAGLPKGEMVVGDRRVPVVTKSGGFG
                     AQDALCRAAEALEVCT"
     gene            complement(1465..2598)
                     /gene="larC"
                     /locus_tag="VFE39_06895"
     CDS             complement(1465..2598)
                     /gene="larC"
                     /locus_tag="VFE39_06895"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HET6258362.1"
                     /translation="MILWLNPFSGISGDMLLGALLDLGAPLDGVRAVVARTGLDGWSL
                     DAERVTRGGVTATRAVVTVHDTATERRAAALLAHIEQAEAPLAARAVRAIAEVEARIH
                     GTDPADVHLHEIGGLDTVVDTVGVAAALHLLDVTEVHCGPLALGAGTVSTRHGVLPVP
                     APATAALLAAVGAQVTGAGDGETVTPTGAALLVAADARFGPVPAMALRGVGYGAGGRD
                     PAGRANVLQALLGGPVGAVATMVLLETNVDDVTGEVLGHLVGQLLEAGAADAWLSPIV
                     MKKGRPAHTVHVLAAPERVADCERIVFAETGSLGLRRSSVDRPALPRSTTTVDVEGHP
                     VRIKHGPWGAKPEHDDVAAAASALGLPLREIARRALTSESART"
     gene            complement(2595..3260)
                     /gene="larB"
                     /locus_tag="VFE39_06900"
     CDS             complement(2595..3260)
                     /gene="larB"
                     /locus_tag="VFE39_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HET6258363.1"
                     /translation="MSDGFEDLGYARADTAREGRLGLPEVVYGPGKTAAQIVGVVRSL
                     LAANDGPVLVTRLTEDVAAAVGAAVPGGEYDPAARLLVWRPAPQRAFRVVVVSAGTSD
                     QPVAAEAAAVAGAVGLEVRELRDVGVAGVHRLLAEVELLAGADAVICIAGMEGALPSV
                     VGGLVGCPVIAVPTSVGYGASFEGVTPLLAMLSSCAPGLVVVNIDSGFGAAIAAHRMA
                     TGR"
     gene            complement(3271..4101)
                     /gene="larE"
                     /locus_tag="VFE39_06905"
     CDS             complement(3271..4101)
                     /gene="larE"
                     /locus_tag="VFE39_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HET6258364.1"
                     /translation="MEPAVDTLLAALRERLRSEQRLFVAFSGGVDSALVAAVAAEELG
                     DRAVAVTAVSASLPRAERAAARDFARGRGIAHVEVDTDELDRPEYVANAGDRCYHCKS
                     ALFDALAPLATLAGARIALGTNLDDLGDHRPGQRAAAARGAISPLVDTGFGKAEVRRV
                     SEAMGLATAHKPAAACLASRVAYGDPVTPELLRRIELAEMAVRELGFPVCRVRAHADG
                     AVARIEVPAGEVPRAAELREALDAGVRAAGFDFCALDLQGFASGRMNVLLGMPARATP
                     "
     gene            4311..4709
                     /locus_tag="VFE39_06910"
     CDS             4311..4709
                     /locus_tag="VFE39_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6258365.1"
                     /translation="MAEPAPLGSEAPAAEAAGDFLDTAITRPRPGAAVLGVQGEVDTL
                     TATLFADAVAELVDGPEPTLVVDLTGVTFLASSGLAVLIQGAHRAEKRNARLRLVVTT
                     RAVRRPLEITGTDQLFDMHSDVHDAIGGDD"
     gene            4778..5218
                     /locus_tag="VFE39_06915"
     CDS             4778..5218
                     /locus_tag="VFE39_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6258366.1"
                     /translation="MSDLDGLSPAETLDGASTVEVRTSASAALIPTIRAVASDLAARA
                     DFDLDAISDLRMAVDEACATLVDVAAPSSTLQCTFLVQSERIEVHAQVRAGHPDAVVS
                     TDTFGWRVLQTLADDVTVHHEPGAGGDGPTVGIRLDKRSGDLPR"
     gene            5215..5994
                     /locus_tag="VFE39_06920"
     CDS             5215..5994
                     /locus_tag="VFE39_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="HET6258367.1"
                     /translation="MTRADPEYGHLAPLLERYSSLEPDHPDREALREELVRGFLPVAQ
                     HIARRFSNRGEPLDDLVQVATVGLINAIDRYSPERGSDFFSFAVPTITGEVRRHFRDL
                     GWSMRVPRRLKDLHVSINGAVSELSQNLGRAPKPSEIAEQLGVPVADVLEGLEASEAY
                     RSSSLDEMLSSEQGSATVGELVGEADAELDRVDFRLALRPVLSELAERERTIVLLRFF
                     GNMTQTQIADRVGISQMHVSRLLAQTLDRLRKRLDPETRVS"
     gene            6031..7275
                     /locus_tag="VFE39_06925"
     CDS             6031..7275
                     /locus_tag="VFE39_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6258368.1"
                     /translation="MTGQPEHVMVVGAGLGGLRTAEQLRSAGFQGRISLVGAEPHAPY
                     DRPPLSKQILTGDWEPEKIVLAELDALLDIGVRVHLGIEAVALRHSEDGYSGWGERGH
                     ELELSDGATLHGDAVVIATGLAARTLPGQPATVHTLRTLDDALALRDALNRIRSLLVV
                     GGGFIGAEVASAARTRGIEVTVLEGLPVVLARALGPDVGAIAGRLLVEGGVDVRTGAA
                     LTRFTDNGTGVGVELADGTHCSADAALVGIGGTPRLEWLDGTGLDLTDGLGCGTTGRV
                     HGLAAAWAVGDVAAWDDPVYGDRHRHEHWTSAADQAAVVASDILGAPPPPPAVPYFWS
                     DQFGLKIQLLGRPELADRVVPLHGTGFDGGPIRGTVGGYLDGDRLVAVVGFGAARIVT
                     RYRAQVAERAPLDTAVSGRGGG"
     gene            complement(7253..7474)
                     /locus_tag="VFE39_06930"
     CDS             complement(7253..7474)
                     /locus_tag="VFE39_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HET6258369.1"
                     /translation="MARDPDTIQREIERARDALADSLDALGEKASPKRLVDAGKESLQ
                     ERLSDPRIRYGLMALGALLAVAFIRRLFR"
     gene            7608..8075
                     /gene="bcp"
                     /locus_tag="VFE39_06935"
     CDS             7608..8075
                     /gene="bcp"
                     /locus_tag="VFE39_06935"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HET6258370.1"
                     /translation="MSTRLAVGDPAPDFTLPDADGKPVSLSDYRGRRVIVYFYPAAST
                     PGCTKQACDFRDNLADLGDAGLDVLGISPDTSAKLAKFRDKEGLTFPLLSDPDKEVLT
                     AWGVFGEKQMYGKTVTGVIRSTFVVDPDGKIEQAQYNVRATGHVAKLRRDLKT"
     gene            complement(8065..9414)
                     /locus_tag="VFE39_06940"
     CDS             complement(8065..9414)
                     /locus_tag="VFE39_06940"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020500429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008706 - 6-phospho-beta-glucosidase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET6258371.1"
                     /translation="MRLTILGGGGFRVPLVYAALAGGSPVTDVVLHDVSASRLGAIRA
                     VLEQMSRPGAPAVHTTTDLDNALAGAQFVFSAIRVDGLAGRTLDERVALAHGVLGQET
                     TGAGGIAYGLRTVPVAMRVAERVAAVAPQAWVINFTNPAGMITEAMQRVLGERVVGIC
                     DSPIGLAQRAARAIEGMGRGSGPRTVDYVGLNHLGWLRGLRVDGVDRLPELLADDDAL
                     CSIEEARLVGPDWVRSIGALPNEYLYYYYCTREAIAAIRSAPSTRGEFLLDQQERFYA
                     AADSTGALQRWSEVRDERDATYMAESREASGAGERDAADITGGGYQQVALELMTALSG
                     GATRTMILNVRNGSAVLGLPAAAVVEVPCLVGPHGVTPLATDPLPGSMLGLLQQVKAV
                     EQDTIEAAVTGSAALALRAFAQHPLVDSVAVARELLAAYRARLPGVAAVLGGGRLRS"
     gene            9728..10777
                     /locus_tag="VFE39_06945"
     CDS             9728..10777
                     /locus_tag="VFE39_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020500431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6258372.1"
                     /translation="MSGMVFLDIVFTGLPTLPAAGTEVWAAGMASCPGGIANLAIAAS
                     RLGLRTSLAAGFGDDVYADFCWRTLAGQERVDLTRSRQFAGWHSPVTVSMAVQRDRSM
                     VTHAHGLPITGDALIGTPPRCRAVLTSIGEGAADPVLPSWVAQARAAGSMVFADAGWD
                     PTGAWDPALLATLAGCDAFMPNAVEAMSYTRTDNPRAALHTLAGHVPLAVVTCGGDGA
                     MAVDAATGEEAVVPALPVDALDPTGAGDVFGAGLVLGTIAGWPLEHRLLFAGVSAALA
                     VHEFGGSLAAPGWGDIGDWWRRVQARAAAGHLGAAELRRRYGFLDDLVPRTHVCGVRR
                     AAATIARQSDLTRPI"
     gene            11462..11536
                     /locus_tag="VFE39_06950"
     tRNA            11462..11536
                     /locus_tag="VFE39_06950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11497..11499,aa:Leu,seq:tag)
BASE COUNT         1628 a         4415 c         4129 g         1500 t
ORIGIN      
        1 gccgtcgatc tcaccggcga gcgcgagggc caccgcccgc tcgacgccct cgaaggcgat
       61 ctgccccgcc tcggcggaga ccttcccgac ggggacggcg acgtcggacg gcacggggag
      121 cacgtcgagc gtccccggat cgaagcgcgc ctcacccgga gtggacacct cgcgcagcgc
      181 gggagcgtcc gggacgacca cagcggcgga tcgcagcgcc cggaggctgc cgatcgcgac
      241 cagcctcgcg acgtcgaccc gcgggtcgac cgccgccttc agggtgacct cgggcccgat
      301 cccggccggg tcaccaaccg tcaggccgag caacggcgtc atgtgcagac ctccaaagcc
      361 tccgcggcgc ggcagagcgc gtcctgcgcg ccgaagccgc ccgacttcgt gaccaccgga
      421 acccggcggt cccccaccac catctcaccc ttcggaaggc cggcggccac ctcggacgtc
      481 aggcggaact cgacggcccc gagggccctc accacggcga gggcggtggc gccaccggtc
      541 aggacgagcc cggcggacgg ggcggcgccg agcacggccg tcacaacccg cgccaggtcc
      601 tcggcgagcg ccgccgcccg cgggctgtcc ggctcgacat caccggtgac ctcgcacgtc
      661 agcacgaccc gccggcccgc gcgcagggcg gcggccgcgg tgtccaccga actcgacgtg
      721 gtgcccacca cgacgagctc ggcgccaccg gcgaggagca gcgccacctg cgccccggtg
      781 gtcgcgtgcg gcgtcccgac gacgacgagc acgccggtcg ccgcggtacg ggggcccggg
      841 gtagggcccg gcgccggcac cagcacgccc gcgagggcac gggacaggcc tcccgagccg
      901 accgcgacgg accccgtgtc gacgacccgg cgcgcgatct ccacgaggtc gtcgtccgtc
      961 tccgcgtcga ccaccagtac accgggcggg aagtggtccg cgacctgctc gatcgtcgtc
     1021 tcgccgtgca cgacgagagc tccgccccgc accgtccggc cctggccggg gaaggccggg
     1081 gtggcgaccg cgccggagcc gcgccatgcg tccagagcga ccgcgacgtc ggcatgcacc
     1141 cggccgcgca tggtggagtc gaccttgacg aacaacatct cggcacccca gccacgcacg
     1201 cgggagaccg cagcgcgcat gcgctccgct gcctcggacg cggggcgcca gcggttgtcg
     1261 gtgagcaggg cgagcacctc ggcgtccggc gccgacgcgt cggacagggc gacggagacg
     1321 gccagcccgc gagcggcgaa cggagctgcg gcgtcggcgg caccggtaag gtcatcggct
     1381 acgacggcca agcgacgcgc agtgccggga gccactcggg cgatgacctc cgtcggccgt
     1441 ctggggtgag ctacggtccc gaggctaggt tcgggcgctt tccgatgtca acgcgcgccg
     1501 agcgatctcc cgcagcggca ggccgagcgc cgacgcggcc gcggcgacgt cgtcgtgctc
     1561 cggcttggcg ccccagggcc cgtgcttgat ccgaaccggg tgcccctcga cgtcgacggt
     1621 ggtcgtgctg cggggaagtg ccgggcggtc gacgctactg cgccgcagcc cgaggctccc
     1681 ggtctccgcg aacacgatcc gctcacagtc cgcgacacgc tcgggtgcgg cgagcacgtg
     1741 cacggtgtgt gccgggcggc ccttcttcat gacgatcggt gagagccacg cgtcagccgc
     1801 gcccgcctcg agcagctgcc cgacgaggtg gccgagcacc tcgccggtga cgtcgtccac
     1861 attcgtctcg agcagcacca tcgtcgcgac cgcgccgacc ggtccgccca gcagcgcctg
     1921 cagcacgttg gcgcggcccg cggggtcccg cccaccggcc ccgtatccga cgccgcgcag
     1981 cgccatcgcg ggcaccggcc cgaaacgcgc gtcggcggcg acgagcaacg cggccccggt
     2041 cggtgtgacc gtctccccgt cacccgcccc ggtcacctgc gcgcctacgg ccgccagcag
     2101 cgccgcggtg gccggcgcag gaaccggcag gacgccgtgg cgggtcgaga cggtgcccgc
     2161 ccccagggcg agcgggccgc agtgcacctc ggtgacgtcg agcaggtgca gcgccgccgc
     2221 gacgcccacg gtgtcgacca cggtgtccag gccgccgatc tcgtgcaggt gcacgtcggc
     2281 cgggtccgtg ccgtggatgc gcgcctccac ctcggcgatc gcccgcaccg cccgggcggc
     2341 cagcggggcc tccgcctgct cgatgtgcgc gagcagcgcg gccgcccggc gctcggtcgc
     2401 cgtgtcgtgc accgtcacca cggccctggt cgcggtcacg cccccgcgcg tcacccgctc
     2461 cgcgtcgagg ctccacccgt cgaggccggt gcgcgccacg accgcgcgga cgccgtcgag
     2521 cggggcaccc aggtccagca acgccccgag cagcatgtca ccggagatcc cgctgaacgg
     2581 gttgagccag aggatcatcg gccggttgcc atccggtgcg ccgcgatcgc cgccccgaaa
     2641 ccggagtcga tgttcaccac gacgagccca ggggcgcagg agctgagcat cgcgagcagc
     2701 ggcgtgacgc cctcgaacga cgccccgtac cccactgacg tgggcacggc gatcaccggg
     2761 caaccgacga gcccgcccac cacgctgggg agggcgccct ccatcccggc gatgcagatc
     2821 acggcgtcgg ccccggccag cagctcgacc tcggcgagca accggtgcac gccggccacg
     2881 ccgacgtccc gcagctcgcg gacctccagc ccgacggcac cggcgactgc tgcggcctcg
     2941 gccgcaacgg gctggtcgga ggtgccggcg gacacgacca cgacgcggaa cgcccgctgc
     3001 ggcgcgggtc gccacacgag cagccgggct gcggggtcgt actccccgcc gggaacagcg
     3061 gcgccgacgg cagcggcgac gtcctcggtc agccgggtca cgagcaccgg gccgtcgttc
     3121 gcggcgagca gggagcgcac gacgcccacg atctgcgcgg ccgtcttgcc ggggccgtag
     3181 accacctctg gcaggccgag ccgcccctca cgcgcggtgt cggccctggc gtagccgaga
     3241 tcctcgaagc cgtcgctcac gtgcggtccc tcacggggtg gcgcgggccg gcatgccgag
     3301 caggacgttc atccggccgc tggcgaaacc ctgcaggtcg agggcgcaga agtcgaagcc
     3361 tgccgcgcgt acacccgcgt ccagtgcctc gcgcagctcc gcggcccggg gcacctcgcc
     3421 ggcagggacc tcgatcctgg ccaccgcgcc atcggcgtgg gctcgcaccc ggcacacggg
     3481 aaaccccagc tcgcgcacgg ccatctcggc cagctcgata cggcgcagca gctccggcgt
     3541 caccgggtcg ccgtaggcca cccgcgaggc gaggcacgcc gcggccggct tgtgcgccgt
     3601 ggccaggccc atggcctcgc tgacccggcg cacctcggcc ttgccgaacc cggtgtcgac
     3661 gagcggcgag atcgcgccgc gcgcggcggc ggcgcgctgg ccgggccggt ggtcgccgag
     3721 gtcgtcgagg ttcgtcccga gggcgatgcg agcgccggcc agcgtggcga ggggagcgag
     3781 cgcatcgaac aacgccgact tgcagtggta gcagcggtcg ccggcgttgg cgacgtactc
     3841 cgggcggtcg agctcgtcgg tgtcgacctc gacgtgggcg atgccacggc cgcgggcgaa
     3901 gtcccgcgcg gccgcacgct cggcccgggg caggctggcc gagaccgcgg tgaccgccac
     3961 ggcccgatcg ccgagctcct ccgcggcgac cgcggcgacc agcgccgagt cgacccctcc
     4021 cgagaacgcc acgaacagtc gctgctcgct gcgcaaccgc tcccgcagcg cggcgagcag
     4081 ggtgtcgacc gccggctcca tgcgcaccta cgacacttta ggtgggcgcc atcccctgcc
     4141 accaccggac gaccggagct gcgcgcgccg gcacaccgag gcgcgggggt aaggtcgtcg
     4201 atgacgttgc cgtgatccct gccgtccgcc ccggggtccg cccggagctc cggacgggtc
     4261 ttcctcgatc cggttcatac agcatccgac cagcgccgga ggtggaacag atggccgaac
     4321 cggcaccact cgggtccgaa gcgcccgctg ccgaggcggc gggtgatttc ctcgacaccg
     4381 cgatcacccg accccggccc ggcgcggctg tcctcggggt gcagggagag gttgacaccc
     4441 tcacagccac gttgttcgcc gatgccgtgg ccgagctggt ggacggcccc gaacccacgc
     4501 tcgtcgtcga cctcaccggc gtgacgtttc tggcatcgag cggcctggcc gtcctcatcc
     4561 agggcgcgca cagggccgag aagcgcaatg cgagacttcg cctggtggtc accacccgag
     4621 cggtgcggcg gccgctggag atcacaggca ccgaccagct cttcgacatg cacagcgacg
     4681 tgcacgatgc gatcggcggc gacgattgat ccaccgcatc ccgggtagca agatgaccga
     4741 tcacgttcac agcacgaccg gaaggagacc cggcagcgtg tccgacctgg acggcctctc
     4801 gcccgccgag acgctcgacg gagcctcgac cgtcgaggtc cggacgtcgg ccagcgccgc
     4861 gctgatcccc acgatccgcg ccgtcgcctc cgacctggcc gcgcgagccg acttcgacct
     4921 cgacgccatc tcggacctgc ggatggccgt ggacgaggcc tgcgccacgc tggtcgacgt
     4981 cgccgcgccg tcgtcgacgt tgcagtgcac cttcctggtg cagtccgagc gcatcgaggt
     5041 gcacgcccag gtgcgggccg ggcaccccga cgccgtcgtc tccaccgaca cgttcggctg
     5101 gcgggtgctg cagaccctcg ccgacgacgt caccgtgcac cacgagccgg gcgccggcgg
     5161 cgacgggccc acggtcggta tccggttgga caagcgctcc ggcgacctac cccggtgacc
     5221 agggccgatc ccgagtacgg ccacctcgct cccctgctcg agcgctactc gtccctggag
     5281 ccggaccacc ccgaccgcga ggcgctgcgg gaggagctcg tgcggggctt cctgccggtg
     5341 gcccagcaca tcgcccgccg gttctccaac cggggcgaac ccctggacga cctcgtccag
     5401 gtggcgacgg tcggcctcat caacgccatc gaccggtact ccccggagcg tggcagcgac
     5461 ttcttctcct tcgccgtccc cacgatcact ggtgaggtgc gcaggcactt ccgcgacctc
     5521 ggctggtcga tgcgcgtacc gcgcaggctc aaggacctgc acgtctcgat caacggtgcg
     5581 gtctcggagc tgtcccagaa cctcggccgt gcgccgaagc ccagcgagat cgccgagcag
     5641 ctcggtgttc cggtcgcgga cgtgctcgag ggactcgagg cctccgaggc ctaccgcagc
     5701 tcgtcgctcg acgagatgct ctcgtccgag cagggcagcg ccacggtcgg cgagctggtc
     5761 ggcgaggccg acgccgagct cgaccgtgtc gacttccgac tggcgctgcg acccgtgttg
     5821 tccgagctgg ccgagcgcga gcggaccatc gtcctgctgc ggttcttcgg caacatgacg
     5881 cagacgcaga tcgccgaccg cgtcggcatc tcacagatgc acgtctcccg gttgctggcg
     5941 cagactctcg atcgcctccg caagcgactg gaccccgaga cccgcgtcag ctgaccgctc
     6001 gtcagcattg ttcctgcgga gggggatgtc atgaccggtc aaccggagca cgtgatggtc
     6061 gtcggcgccg gactcggcgg cctgcgcacc gccgagcagc tgcgctcggc agggttccag
     6121 gggcggatca gcctcgtcgg cgccgagccg cacgcaccct acgaccggcc gccgctgtcc
     6181 aagcagatcc tcaccgggga ctgggagccc gagaagatcg tgctggccga gctcgatgcg
     6241 ctcctcgaca tcggggtccg ggtccacctg ggcatcgagg ccgtggcact gcggcacagc
     6301 gaggacggct acagcgggtg gggcgagcgc ggccacgagc tggagctgtc cgacggcgcc
     6361 acgttgcacg gcgacgccgt cgtgatcgcc accggcctcg cggcgcggac actgcccggc
     6421 cagcccgcga ccgtgcacac cctgcgcaca ctggacgacg cactcgccct ccgggacgcg
     6481 ctgaaccgga tccggtcact gctcgtcgtg ggcggtgggt tcatcggcgc ggaggtggcg
     6541 agcgcggcca ggacccgcgg gatcgaggtg acggtgctgg aggggctgcc ggtggtcctc
     6601 gctcgcgcgc tcggccctga cgtcggggcg atcgccggtc ggctgctcgt ggagggcgga
     6661 gtcgacgtgc gcaccggcgc ggcgctcacc cggttcaccg acaacggcac cggcgtcggc
     6721 gtcgagctcg ccgacggcac gcattgctcc gcggatgccg cgctcgtcgg tatcggcggc
     6781 accccgcggc tggagtggct cgacggcacc gggctcgacc tcaccgacgg gctcggctgc
     6841 ggaaccacgg gccgggtgca cgggctggcc gcggcctggg cggtaggcga tgtcgccgca
     6901 tgggacgacc cggtgtacgg cgaccggcac cgccacgagc actggacgag cgcagccgac
     6961 caggccgcgg tcgtggccag cgacatcctg ggcgcccctc ctccgccccc ggccgtgccg
     7021 tacttctggt ccgaccagtt cggactcaag atccagctcc tcggtcggcc ggagctggcg
     7081 gaccgggtgg tgccgctgca cggcaccggg ttcgacggcg gccccatccg tggcaccgtc
     7141 ggcgggtacc tcgacggcga ccggctggtg gccgtggtcg gtttcggcgc ggcccggatc
     7201 gtcacccgct atcgcgctca ggtggccgag cgcgcccctc tagacaccgc ggtcagcgga
     7261 agaggcggcg gatgaaagcc acggcgagca gggctcccag ggccatgagg ccgtagcgga
     7321 tcctcgggtc gctgagccgc tcctgaaggc tttccttgcc cgcgtcgacg aggcgcttcg
     7381 ggctggcctt ctcacccagg gcgtccaggc tgtccgccag cgcgtccctg gccctctcga
     7441 tctcgcgctg gatggtgtcc gggtcgcgcg ccacggtgcc tcctactttc gcactgcctt
     7501 ggcaaggtca cgaggaagtg ccaccgtaga tcacgcctgc cccatccgtg ggggcggccg
     7561 ggcgggtcgg cgcgccgccc cccggccgtg gtgccagtat cgctcgcatg agtacgcgac
     7621 tggctgtcgg tgaccctgcc ccggacttca cgctccccga cgccgacggc aaaccggtgt
     7681 ccctgtcgga ctaccgcggc cgccgggtca tcgtctactt ctaccccgcg gcgagcactc
     7741 cgggctgcac gaagcaggca tgtgacttcc gggacaacct cgccgacctc ggcgacgccg
     7801 ggctggacgt cctcggcatc tcgccggaca catcggccaa gctcgcgaag ttccgcgaca
     7861 aggaggggtt gaccttcccg ctgctgtccg acccggacaa ggaggtcctc accgcgtggg
     7921 gcgtcttcgg ggagaagcag atgtacggca agaccgtcac cggggtgatc cgctcgacct
     7981 tcgtggtcga cccggacggg aagatcgagc aggcgcagta caacgtccgc gccacggggc
     8041 acgtggcgaa gctgcgcagg gacctcaaga cctgaggcgc ccgccgccga gtaccgcggc
     8101 caccccgggt agccgcgccc ggtaggcggc cagcagctca cgcgcgacgg cgacggagtc
     8161 caccagcggg tgctgggcga aggcgcgcag cgccagggcc gccgaccccg tgactgcagc
     8221 ctcgatggtg tcctgctcca ccgccttgac ctgctgcagg agcccgagca tcgagccggg
     8281 cagcgggtcg gtggccagcg gcgtgacccc gtgcgggccc accaggcagg gcacctcgac
     8341 gaccgcggcg gccggcagcc cgagtaccgc cgagccgttg cggacgttga ggatcatcgt
     8401 gcgggtggcg ccgccggaca gtgcagtcat gagctcgagc gcgacctgct ggtagccccc
     8461 gcccgtgatg tcggcagcgt cgcgctcgcc ggcaccgctg gcctcgcggc tctccgccat
     8521 gtaggtggcg tcgcgttcgt cgcgcacctc cgaccagcgc tgcagggcgc cggtggagtc
     8581 tgccgctgcg tagaaccgct cctgctggtc gagcaggaac tcgccccgcg tgctcggtgc
     8641 ggaccggatc gcggcgatgg cctcgcgcgt gcagtagtag tagtacaggt actcattggg
     8701 gagggcgccg atgctccgga cccagtccgg gcctaccagc cgcgcctcct cgatcgagca
     8761 cagcgcgtcg tcgtcggcga gcagttcggg gagccggtcg acgccgtcca cccgcagccc
     8821 gcgcagccag cccaggtggt tgagccccac gtagtcgacc gtgcgggggc cactgccccg
     8881 gcccatcccc tcgatcgcgc gtgccgcgcg ctgtgccagc ccgatcggtg agtcgcagat
     8941 gccgacgacc cgctcgccga gcacgcgctg catcgcctcc gtgatcatcc cggccgggtt
     9001 ggtgaagttg atgacccacg cctgcggggc gacggcggcc acccgctccg ccacccgcat
     9061 cgccaccggg accgtacgca gcccgtaggc gattccgccc gcgccggtgg tctcctgacc
     9121 caacaccccg tgggccagcg cgacgcgctc gtccagggtg cggcctgcaa gcccgtccac
     9181 ccggatcgcc gagaacacga actgtgctcc ggccagcgca ttgtcgaggt cggtggtggt
     9241 gtgcacggcg ggtgcgcccg gccgggacat ctgctccagc accgcgcgga tcgcccccag
     9301 ccgggacgcc gatacgtcgt gcaggacgac gtcggtgacc ggtgagccgc ctgccagcgc
     9361 cgcatagacc agcggcaccc ggaacccgcc accaccgagg atggtgagcc tcacgcgaca
     9421 gcctctaccg acggaagcac gggccgacgg tagcgcaggg cgcgcccgca ccgacgtggt
     9481 cgacggcacg cgccagattg cggcaggagc gcgcaggcgc gttgccaggc gcgtggatca
     9541 ggcggcacta tccgacggag cagcacatcg gagggggatg agttcgccgg tgaccagcgc
     9601 agcgccgaac acggcgtccg agccggacgc gttgccggca gggccgctcg ccacgccgca
     9661 cgaggcggtc tggtgggatc cgctcgtcga cgtccgcggc gccgactggc cctccgtcga
     9721 cgtgttcatg tccggcatgg tgttcctgga catcgtgttc accggcctgc ccacgctgcc
     9781 cgcggcaggc accgaggtgt gggccgcggg gatggcctcg tgccccggcg gcatcgcgaa
     9841 cctggccatc gcggcgtccc ggctcggtct ccgcaccagt ctcgcggccg gcttcggcga
     9901 cgacgtctac gccgacttct gctggcggac gctcgcgggg caggaacggg tcgacctgac
     9961 gcggtcgaga cagttcgcgg ggtggcactc ccccgtgacc gtctcgatgg cggtgcagcg
    10021 cgaccggagc atggtcacgc acgcgcacgg cctcccgatc actggggacg ccctcatcgg
    10081 cacaccgccg cgatgccgag cggtgctcac cagcatcggc gagggtgcgg ccgacccggt
    10141 gctgccctcc tgggtggccc aggccagggc ggcaggctcg atggtcttcg ccgacgctgg
    10201 ctgggacccc accggcgcct gggatccggc cctgctcgcc acgctcgccg ggtgcgacgc
    10261 attcatgccc aacgccgtgg aggccatgag ctacacccgc accgacaacc cccgcgcagc
    10321 gctgcacaca ctcgccggcc acgtcccgct cgccgtcgtg acctgcggcg gcgacggggc
    10381 gatggccgtg gacgccgcca ccggtgagga agccgtggta cccgccctgc cggtcgacgc
    10441 cctcgacccc accggcgcgg gcgacgtgtt cggggccggc ctggttctgg gcacgatcgc
    10501 cggctggccg ctggaacacc ggctgctgtt cgccggcgtc tccgcggcgc tcgccgtgca
    10561 cgagttcggc gggtcattgg ccgcccccgg ctggggtgac atcggcgact ggtggcggcg
    10621 ggtacaggcc cgggccgcgg cgggccacct cggcgccgcc gagctgcggc gccgttacgg
    10681 gttcctcgac gacctggtgc cccggaccca cgtctgcggc gtccggcgcg ccgccgccac
    10741 catcgcccgg cagtcggacc tgacccgccc gatctgaccg accgaccgcg cgttcgaaca
    10801 tcagttcgat atgatcacgg catgccccgc tcccggagtt ggaccgacga gcaactcgcc
    10861 gctgccgtgg cggcgagccg cacgctgagc gaggtgtgcg ttcgcctcgg cctgcagccc
    10921 gggaagtacg acgtgctgcg caggcatatc cgtcgcctcg gactggacgc ctcgcatatc
    10981 ccgcgatccg caatgggcgc gccacgaacg agccggggct ggtcggacga ggacctcgcg
    11041 gttgcggtgg ccgcgagcga ctcagtctcg gaggtcagcc gcaggttggg ctatacgccg
    11101 aacggcggcg tccaccggat gatcgtccac cgcattcgca cgttcggtct cgacacgtca
    11161 catttcaccg gacagcggtg ggcccgcggt cgcacccttc cccggaaatc gataccgctc
    11221 gaggagatcc tcgtcccgct ctcgacctat cgcaccagct ctcggctacg ccgacggctc
    11281 atcgccagcg ggttgctcaa ggcggagtgc gcgaactgcg ggctcgcctc ctggcgcggc
    11341 aggccgttac cactccatct ggatcacatc aacggcgacc acaccgacaa tcggctcgag
    11401 aacctccgta tcctgtgccc caactgccac tcccagaccg acacctggtg cagtggcaag
    11461 ggccggcgta gcccaactgg caggaggccc tccgtttagg gcggagacag tgtgggttcg
    11521 aatccctccg ccggcacagg tttttcccag ctatcagggc tggtccgtcc cgggttcctg
    11581 ctgcacggtc gtggacgtcg gagtgcccgg cggcacggcc ggttgccggc gcgccaccag
    11641 cagcgccccg gccagcccca gcaggcccga cg
//
LOCUS       DASRSC010000145        14153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5288829,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000145 DASRSC010000000
VERSION     DASRSC010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 14153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14153
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5288829"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..156)
                     /locus_tag="VFE39_06955"
     tRNA            complement(84..156)
                     /locus_tag="VFE39_06955"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(121..123),aa:Ala,seq:cgc)
     gene            complement(204..1202)
                     /gene="glpR"
                     /locus_tag="VFE39_06960"
     CDS             complement(204..1202)
                     /gene="glpR"
                     /locus_tag="VFE39_06960"
                     /inference="COORDINATES: protein motif:HMM:NF045516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase receptor GlpR"
                     /protein_id="HET6258373.1"
                     /translation="MPSSMIFASLVVLWLLILVPAVARHRQEVARPTVAALSGRVLER
                     PRQRHGSETGRELEVDVMDEVDQEPSTTSETDREDGEDGEDDRADEVDHDDRGWERPP
                     PRYRPGRGGFDPEAAALAARARYAFRQRVVLAMLIVAFTTGVIAAMAVQAFWWAHGVV
                     DVVLVGYLVYLRRQVRLEEAIRERRAARMAGTRRQQAADDPDLDEWARRGREMTRRPQ
                     DDGYDGDRYPEDGDEAGLDGDTDPGDGGSDDLTRERPGRVRGAGEPLHSEQDRSAGGD
                     ESLSDPIEGEPALPRLRPAPPPPLPAGTALVDATEDDPELHDLTGPGRPRYRRAAG"
     gene            complement(1415..2080)
                     /locus_tag="VFE39_06965"
     CDS             complement(1415..2080)
                     /locus_tag="VFE39_06965"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET6258374.1"
                     /translation="MAHAYPAPITGRHPGWPATLGALRVAAGVVALRPVRLRDGRTWS
                     AIRIRDERHLAPWEPTMPGGWAQRHAVSEWPARWLQLRGAGRRGAVLPFAITLDGRFV
                     GQVMVGNVVREPLLSAYVGYWIDSAVAGQGVTTAAVALVVDHCFGRVGLHRLEATVRP
                     ENVASLRVLAKLGFRKEGLFKRYLDVDGAWRDHYCFAVTVEDLVSSSLAGRLVADGRA
                     ERA"
     gene            complement(2088..3314)
                     /gene="glp"
                     /locus_tag="VFE39_06970"
     CDS             complement(2088..3314)
                     /gene="glp"
                     /locus_tag="VFE39_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET6258375.1"
                     /translation="MRLVDEQLERVLASAVKPAPVRVAISEAHGLRCAEEVVTARPLP
                     GFDQAAIDGYAVRSVDLVGASEHEPVSVPVVGEIPAGSRQPLRLQPRQAVRVAAGAPL
                     PTLADAVVPVGFTDGGSARLMVHCGVPSAAFVRRIGEDVQPGDVAVRRDAVIGAAQVA
                     LLAAAGREKVLVHPKPRVSVISVGDELVDLARSPGAGQVADVCSHALTAAAREAGAET
                     SRASLVRGDARDIGAAMQDRLPLSDVIVVCGAVGGNAGAEVHAAFAGLGELDTTRVAM
                     HPGSTQAFGRLGPDGVPTFLFPSHPATALLLFEVMVRPLLRLALGRANPYRRMLSARL
                     TSPVSSTPGRRGYVRGRLLREQATGDYLVQPLGTSGTHLLSSITEANGLIVLPEHVTE
                     ATVDEVVTVAFLPAPA"
     gene            complement(3321..4235)
                     /locus_tag="VFE39_06975"
     CDS             complement(3321..4235)
                     /locus_tag="VFE39_06975"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HET6258376.1"
                     /translation="MREATSESSARSALPFRSAVVPAAGLGTRFLPTTKTVPKELLPV
                     VDTPGIELVAAEAAEAGAEVLLIVTSPGKDAVAAHFADSSELEKELQARGKDALVAKV
                     RRANELIAVRTVTQDDPKGLGHAVGCARDAIGDDEQAFAVLLPDDLVLPTGVLTKMAE
                     VREQHGGSVLCAFDVPREQIAAYGVFEVSDTGDDDVKRVHGMVEKPAPEEAPSTLAAA
                     GRYLLDRAIFDALDRITPGAGGELQLTDAVALLISEGHPVHVVVHRGGRHDLGNPGGY
                     ARAFVDFALEDPDYGPDLRAWLAVRLNG"
     gene            4272..4904
                     /locus_tag="VFE39_06980"
     CDS             4272..4904
                     /locus_tag="VFE39_06980"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET6258377.1"
                     /translation="MTARANRTERAVRAAKAQWRARLTAARLALPVEVRAARAAALAE
                     AAVALAAGTGGPVCAYLPVGSEPGSPELVAALHAAGHEVLLPVVPAVRGPLDWARYDG
                     TAAVGLGPLGLREPTGPRRGPTAIAAARLVLVPALAADRGGRRLGRGGGYYDRTLPLA
                     NPGVPLVVVLNDEELVDELPDEPHDRRVTAALLADAGHTRLGNGPLGNRE"
     gene            4975..5334
                     /locus_tag="VFE39_06985"
     CDS             4975..5334
                     /locus_tag="VFE39_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HET6258378.1"
                     /translation="MPTYQYACTACNHRFEAVQSFSDASLTECPVCAGHLRKVFSSVG
                     IVFKGSGFYRTDSRSGAGGTVAEAAKTETASESKSESKSESKSGTNGAASTSGSSSKD
                     SSSSSSSSSAAPATAAS"
     gene            5434..6075
                     /locus_tag="VFE39_06990"
     CDS             5434..6075
                     /locus_tag="VFE39_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HET6258379.1"
                     /translation="MTNVRLAPRLRDRLGALLVGPGWRRLALLRRSAAAVLAVAALVL
                     ALAPRTGAGGVPLVVTAVDLPAGSMLLAEQLAVREWPADLTPTGAVREVAGAAGRVLV
                     GAARSGEPLTDVRLAGSGLALGGGAGAAAVPVRLADAGVAAVLVPGSRVDVVTVGART
                     DEPIVLAAAVTVLAVLPEDSRTSGRLVLVATAPGQAARVAAAALADQVAITLR"
     gene            6077..6478
                     /gene="mscL"
                     /locus_tag="VFE39_06995"
     CDS             6077..6478
                     /gene="mscL"
                     /locus_tag="VFE39_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET6258380.1"
                     /translation="MIKGFKDFVLRGNVVELAVAVVVGAAFGSIVTAFTSGIIQPLIN
                     AVTPPSSPGFGIQLVGGKGSTYIDFAAVIGAALNFLIVAAVVYFVIVFPLTALKERRK
                     RGVDAGPTEPTDVELLTEIRDLLREQSQRRG"
     gene            complement(6485..6649)
                     /locus_tag="VFE39_07000"
     CDS             complement(6485..6649)
                     /locus_tag="VFE39_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258381.1"
                     /translation="MDELSDQARRRLDEVFGDVLPSTTADERDERDEPAPAAADDELL
                     ANRPPHHDRD"
     gene            6683..7144
                     /locus_tag="VFE39_07005"
     CDS             6683..7144
                     /locus_tag="VFE39_07005"
                     /inference="COORDINATES: protein motif:HMM:NF025379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET6258382.1"
                     /translation="MGLTEKAKELADVALEKAGVYSEKAAAKAAELSDVARDKAPGYL
                     DRAAELAGRAVDSAAAGVDKATGGRFHEQIEGASTKVGESLDRARHTTAAPTGVPDEA
                     PGPVIVPPAPADVAGQAGAPVTPAATNPEATKPDARDTGGTDTGSTPGPKS"
     gene            complement(7177..7671)
                     /locus_tag="VFE39_07010"
     CDS             complement(7177..7671)
                     /locus_tag="VFE39_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HET6258383.1"
                     /translation="MEMAPPQIGRALVVVVDDRVSHGEREDAIGPLVTELLEEAKLVV
                     DAVVVVPGDPVAIRNALNTAVIGGVDLVVTVGGTGVSPRDVTADATAGVLDRPIPGIA
                     EAIRASGLAAGAVDAGLSRGLVGVSGSTLVVNLAPSRAAVRDGMATLTPLVAHVISEL
                     SGLD"
     gene            complement(7822..8865)
                     /locus_tag="VFE39_07015"
     CDS             complement(7822..8865)
                     /locus_tag="VFE39_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6258384.1"
                     /translation="MREELHAVVPVALAFSVPIAALGAVLLHRMRRRSIMAAVTVLVL
                     VPVIATMVGVIGVSGFMYTPQLAGAVVACGAVAIVTVPAAMMLGRGIASDVMWLHEAR
                     DAERAAEASRRELVTWISHDLRTPLAGIRAMSEAISDGIVADADEVTDYARRIRGETV
                     RLSGMVDDLFQLSRITSGALHLTLSAVPLREVVSEAVAVEGVAASRKGVVVRAEEHGD
                     WPVVLGSDPELARVVRNLLSNAIRHTPPAGAVVVAAGVTGDKAWLRVDDACGGIPEDD
                     LQRVFDVAFRGVTARTPEERSGGGLGLAIARALVEAHDGDIHAHNAGPGCRFEVLLPL
                     ASTPTTDRPGPVR"
     gene            complement(8862..9569)
                     /locus_tag="VFE39_07020"
     CDS             complement(8862..9569)
                     /locus_tag="VFE39_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6258385.1"
                     /translation="MTDRPLALVVDDDVTVREVVTRYLDRSGYRVEVAGDGEQALRAV
                     AGQVPDIVILDLMLPRVGGLEVCRRLRRETTGVPIVMLTALGEEEDRVLGLELGADDY
                     VTKPFSPRELVLRVASVLRRSREVPGRDAATAPVVDGDLRVDVLGRRASLAGRALALT
                     VREFDLLAFLARRPGQVFTRAELLELVWGWDFGDQSTVTVHVRRLREKVEADPTKPAR
                     IATVWGVGYRYDGSQSP"
     gene            complement(9867..11108)
                     /locus_tag="VFE39_07025"
     CDS             complement(9867..11108)
                     /locus_tag="VFE39_07025"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HET6258386.1"
                     /translation="MANETPDRDATPTGPNERVTPGEASSHGGGFSYPGGPYPGSYGT
                     AQYPQPNAPWPGPVTPPPGRRRSARMAAFGLVGALLAGGAGGALGYSLAESRDGGASN
                     GVLGSPLPPIDGTAPMSPVEAVAERVLPGVVQLRVDSGGSSGEGSAMVLSADGLLLTN
                     NHVVEAAANRGRVTAVFQNGDTAQAAIVGRDPSSDLAVIRAQGVSRLTPIELGNSDSV
                     RVGQQVVAFGAPLGLGGTVTTGIISALNRPVSVGGDQGASEATVLNALQTDAPINPGN
                     SGGPLVDMQGRVIGINSAIATTGQQGGSIGVGFSIPINQARRVAEELERTGKASRAVL
                     GVSVGDDPQVSGARIQSVAPGSAAEAAGIRAGDLVIGFDGQRILAGDGLQAAVRSRAP
                     GQVVEVQLADRSVSVTLGAAS"
     gene            complement(11190..12716)
                     /locus_tag="VFE39_07030"
     CDS             complement(11190..12716)
                     /locus_tag="VFE39_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6258387.1"
                     /translation="MSERAGPGSDAVGAHAATPGDATNDDATPGNGPAERVRQGRPDG
                     STPRIEAFDRVSLRARIGVLAALVAALAVILVSVAAFVTVRVNIMQTLDANLLQRASA
                     AAQSELADPRQLATIPTGVLGAGDIRLALLLANGRASSAEGQASAPPLGADELEVAQG
                     IETSSVRTAAEDGVQYRVVAVQAGPGQALVIAQRLDSTQQVLTKLGVALPIVGGIGVV
                     LAALAGVVVARAGLRPVDRLTAATERVAATNDLRPIPVDGSDELARLTHSFNDMLGAL
                     AASQEQQRRLVADAGHELRTPLTSMRTNLELLAAANRPGAPMLPESDRAEILEDVQAQ
                     VTELSTLVGDLVELARDDVPLVVHEPLELTDVVLRSLERARRRAGAVEFVPMLVPWTL
                     VGDSTALERAVLNLLDNAAKWSPPGGTARVQMRPLDEWSIVLEVADAGPGIAEEDLPH
                     VFDRFYRSATSRTMPGSGLGLSIVRQVAVRHGGAVWAGRAPEGGALLAVRLPGRQPRF
                     "
     gene            complement(12713..13411)
                     /locus_tag="VFE39_07035"
     CDS             complement(12713..13411)
                     /locus_tag="VFE39_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6258388.1"
                     /translation="MRILVVDDDRSVRDSLRRSLAFNGYQVELANDGQAALDAVLIQR
                     PDAMVLDVMMPRLDGLEVCRRLRGGGDDLPILVLTARDAVSDRVAGLDAGADDYLPKP
                     FALEELLARLRALLRRRTPDEIAAAAGQSKPLEFWDLSLDPDTRDVRRGDRPISLTRT
                     EFSLLELLLAHPRRVLTRAQILEQVWGYDFPTTGNALEVYIGYLRRKTEAEGEPRVIH
                     TVRGVGYVLRDTPP"
     gene            complement(13556..>14153)
                     /locus_tag="VFE39_07040"
     CDS             complement(13556..>14153)
                     /locus_tag="VFE39_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6258389.1"
                     /translation="INYVVPGIVLLCTGYGASSTAMSVASDMTSGMIDRLRSLPIRSS
                     AVLTGHVTASVARNALATTIVIVAAIAMGFRPTATLSDWALAAGLLLLYVLAMSWLAA
                     GLGVIARSVESASVLSFFMLFVPYLSSAFVPTDTMPSFLQGISEHQPITPVIETVRGL
                     LTGTPLGDRGWVALAWTVGILVVTFTGSTWLYRHRTRG"
BASE COUNT         2041 a         5226 c         4980 g         1906 t
ORIGIN      
        1 agccatcgtg atctggcccg atcggaccct agggccgtaa agccgctgtc aggcagcaga
       61 ggtgtccgat gcttcggtcg gagtggagct gaggggaatc gaacccctga cctactcgat
      121 gcgaacgagt cgcgctacca actgcgccac agccccgatg cccgtcgttc ccgccgggcg
      181 tgaaccacac tatcagccgc ttactacccc gccgcacggc ggtagcgcgg ccgacccggg
      241 cccgtcagat cgtgcagctc ggggtcgtcc tcggtggcgt cgaccagcgc tgttcccgcc
      301 ggaagcggcg gtggcggcgc agggcgaaga cgcggcagcg cgggctcgcc ctcgatgggg
      361 tcggagagcg attcatcgcc gcctgcggaa cggtcctgtt cggaatggag cggttcgcct
      421 gctccgcgga cacgccctgg ccgctcccgg gtgaggtcgt ccgaaccgcc gtccccggga
      481 tcggtgtcgc catcgagccc ggcctcgtcc ccgtcctcgg ggtaacggtc gccgtcgtat
      541 ccgtcgtcct gtgggcggcg ggtcatctcg cggccgcgcc gggcccactc gtcgaggtcg
      601 gggtcgtcgg cggcctgctg acgccgggtg cccgccatcc tggcggcccg ccgctcccgg
      661 atcgcttcct ccagccgcac ctggcggcgc agatagacga gatacccgac caggacgacg
      721 tcgaccacgc cgtgcgccca ccagaaagcc tgcacggcca tggcggcgat cactccggtg
      781 gtgaacgcga cgatcagcat cgcgagcacg acccgctgac ggaacgcgta gcgggcgcgg
      841 gcggcgagag cggccgcctc cgggtcgaat ccgccgcgcc cgggacggta ccgcggcggg
      901 ggccgctccc atccacggtc gtcgtggtcc acctcgtcgg cccggtcgtc ctcgccgtcc
      961 tcgccgtcct cgcggtcggt ctcgctcgtc gtgctgggtt cctggtccac ctcgtccatc
     1021 acgtccacct ccagctcgcg gcccgtctcc gaaccatggc gctgccgtgg tcgctcgagc
     1081 acccgtccgg agagcgcagc cacggtcggg cgcgccacct cctggcggtg tcgcgcgacc
     1141 gcaggaacga ggatcagcaa ccacagaaca accaggctcg cgaagatcat cgagcttggc
     1201 acggctgctt cctccccacg gcgcagtccg gtcgcgactc ggtctccccg gccaagatcg
     1261 cttcccgaac ccacaacggt agtgagcggg accggaggtt gtgggcagcg acgcgccgtg
     1321 gcgacgaggt ttactcatga gcaccagggg gttcttgggg cggatgtgat ctttgcgagg
     1381 ggccgaatgg caggcaaatg gtcgcggagc gtggtcaggc tcgttctgcc cgaccatccg
     1441 caacgagccg tccggcgagg ctgctcgaca cgaggtcctc gaccgtgacc gcgaagcagt
     1501 aatggtcccg ccaggcgccg tcgacgtcca ggtaccgttt gaagaggccc tccttgcgga
     1561 atccgagctt cgccagcacc cggaggctcg cgacgttctc cggccgcacc gtcgcctcca
     1621 gccggtgcag cccgacccgg ccgaagcagt ggtccaccac cagagccacg gccgccgtgg
     1681 tcacgccctg gccggccacg gcggagtcga tccagtaccc gacgtacgcc gacagcagcg
     1741 gctcgcgcac gacgttgccg accatcacct gcccgacgaa gcgcccgtcc agggtgatcg
     1801 cgaacggcag caccgcacca cgcctgccgg ccccccgcag ctgcagccag cgcgccggcc
     1861 actcgctcac ggcgtgccgc tgggcccagc cgccgggcat cgtcggctcc catggcgcga
     1921 ggtggcgctc gtcgcggatc cggatggccg accacgtcct gccgtcgcgc agccgtaccg
     1981 gtcgcaacgc caccacgccg gcggcgacgc ggagggcgcc gagagttgcg ggccagccgg
     2041 ggtggcgccc ggtgatcggc gccggatagg catgagccat tgcgggttca ggccggagcc
     2101 ggcaggaagg ccacggtgac cacctcgtcc accgtggcct ccgtgacgtg ctccggcaac
     2161 acgatgagcc cgttggcctc ggtgatcgag gacagcaggt gcgtgccgga cgtgccgagc
     2221 ggctgcacga ggtagtcccc ggtggcctgc tcgcgcagca gccgaccgcg cacgtagccg
     2281 cggcgtcctg gggtggagct gacgggtgag gtgagccggg ccgagagcat gcgccggtac
     2341 ggattggccc ggccgagcgc gagccgcagc agcgggcgca ccatcacctc gaacaggagc
     2401 agggccgtgg ccgggtgcga tgggaacagg aaggtcggga cgccatccgg gccgaggcga
     2461 ccgaacgcct gcgtggaacc gggatgcatc gcgacgcggg tggtgtcgag ttcgccgagt
     2521 ccggcgaacg cggcgtgcac ctcggcgccc gcgttgccgc cgacggcccc gcacaccacg
     2581 atgacgtcgc tgagggggag ccggtcctgc atcgccgcac cgatgtcgcg ggcgtcgccg
     2641 cggacgagac tcgccctcga tgtctccgct ccggcctcgc gcgcggcggc ggtgagcgcg
     2701 tgcgagcaga cgtcggccac ctgcccagcg ccaggcgacc gtgccaggtc cacgagctcg
     2761 tcaccgacgg agatcaccga gacccgcggc ttcgggtgca cgagcacctt ctcccgcccg
     2821 gcggcggcga gcagggccac ctgcgccgcc ccgatcaccg cgtcccggcg caccgcgacg
     2881 tccccgggct gcacgtcctc cccgatccgc cgcacgaacg cggccgaggg cacgccgcag
     2941 tgcaccatga ggcgcgcgct gcccccgtcc gtgaacccga ccgggacgac ggcatcggcc
     3001 agggtcggga gcggcgcccc ggccgccacc cgcacggcct gtctcggctg cagtcgcagc
     3061 ggctgccgag acccggccgg gatctccccg accaccggaa ccgacaccgg ctcgtgctcg
     3121 ctcgcaccga ccaggtcgac gctgcgcacg gcgtaaccat cgatcgccgc ctggtcgaaa
     3181 ccgggcagtg gccgtgccgt gacgacctct tccgcacacc gcaatccgtg tgcctcggag
     3241 atcgccaccc gcacgggagc cggcttgacc gcgctcgcca gcacccgttc gagctgctcg
     3301 tcgaccaatc gcacgggagg tcagccgttc aggcggacgg ccagccacgc acgcaggtcg
     3361 ggaccgtagt ccggatcctc gagtgcgaag tcgacgaacg cccgcgcata accgcccggg
     3421 ttacccagat cgtgtcgccc accgcggtgc accaccacgt gtacagggtg cccttcggag
     3481 atcagcagcg cgacggcgtc ggtgagctgc agctcgccgc ccgcgcccgg ggtgatccgg
     3541 tccagggcgt cgaatatcgc gcggtcgagc aggtagcggc ctgcggccgc cagcgtcgac
     3601 ggcgcctcct cgggtgcggg cttctcgacc atcccgtgca cccgcttgac gtcgtcgtca
     3661 ccggtgtcgc tgacctcgaa caccccgtag gccgcgatct gctcgcgcgg gacgtcgaaa
     3721 gcgcagagca cgctgccgcc gtgctgctcg cgaacctccg ccatcttcgt gagcacaccg
     3781 gtcggcagca ccaggtcgtc gggcagcagg acggcgaatg cctgctcgtc gtcgccgatc
     3841 gcgtcgcgcg cgcagcccac cgcgtgaccg aggcccttcg gatcgtcctg cgtgacggta
     3901 cgcacagcaa tcagctcgtt ggcgcggcgg accttcgcca ccagcgcgtc cttgccccgc
     3961 gcctggagtt ccttctccag ctccgaggaa tcggcgaaat gcgcggccac cgcgtccttg
     4021 cccggcgatg tgacgatcag caggacctcg gctcccgctt ccgcggcctc ggccgcgacc
     4081 agctcgatac cgggtgtgtc gacaacgggc agcagttcct tgggcacggt cttggtcgtg
     4141 ggcaggaatc gggtgcccag acccgcggcc ggaaccacgg ccgagcggaa cggcagcgca
     4201 cttcttgccg atgattcgct cgtagcctct ctcatggtcg gcgagcctat cggcgccggt
     4261 agcgtggccg cgtgacggca cgcgcgaatc ggaccgaacg ggcggtccgc gcggcgaagg
     4321 cgcagtggcg ggcccgcctc accgcggccc gcctggcact tccggtcgag gtgcgggccg
     4381 cgcgggcggc ggcgctggcc gaggctgcgg tggcactggc cgccggcacc ggcggtccgg
     4441 tgtgcgccta cctgccggtg ggctccgagc ccggctcgcc cgagctggtg gcggccctgc
     4501 acgcggcagg ccatgaggtg ctgctgccgg tcgtgcccgc ggtgcgcggt ccactcgact
     4561 gggcccgcta cgacggcacg gccgcggtcg gcctcggccc gctcgggctg cgggaaccga
     4621 ccggaccccg tcgcggcccc accgcgatcg ccgcagcccg actggtgctc gtccctgccc
     4681 tcgccgcaga tcgcggcggt cgcaggctcg gccgtggcgg cggctactac gaccgcaccc
     4741 tgccgctggc gaacccggga gtgccgctgg tcgtggtgct caacgacgag gaactggtgg
     4801 atgaactccc cgacgagccg cacgaccggc gggtcacggc cgcgctcctc gccgacgccg
     4861 gtcacactcg gctggggaac ggtcctctgg ggaacaggga gtgaccccga cgagttatgc
     4921 tggcactcag tgcgttcgag tgccagctcg tcgagtgcca gtccggaggg acacgtgccg
     4981 acctaccaat acgcctgcac cgcctgcaat cacaggttcg aggcagtgca gtccttcagt
     5041 gacgcgtcgc tgaccgagtg ccccgtgtgt gcggggcacc ttcgcaaggt gttctcgtca
     5101 gtgggaatcg tgttcaaggg cagcggtttc taccgcaccg acagccgctc cggcgcgggt
     5161 ggcaccgtgg ccgaggcggc caagacagag acggccagcg agtcgaagtc cgagtcgaag
     5221 tccgagtcga agtccggcac caacggcgcg gcaagcacgt ccggtagcag cagcaaggac
     5281 agctcctcga gcagctcgtc ctccagcgct gctccggcta ccgccgcctc ctgatcacac
     5341 cggagcggct gggctgtcca catccccaca cggttatcca caggctccgc gatcaccagg
     5401 ccatgagcgt ctccgcgccc tagcgtcgac gccatgacaa acgtccggct cgcgccgcgg
     5461 ctgcgggatc gcctcggcgc gctgctggtc ggccccggtt ggcgcaggct ggccctgctc
     5521 cgcaggtcgg ccgcggcagt gctcgccgtg gccgcgctgg tgttggcgct cgcgccgcgt
     5581 acgggggcgg ggggtgttcc cctggtggtc acggctgtcg atctacctgc cggcagcatg
     5641 ttgctggccg agcagctcgc ggtccgcgag tggcccgccg acctcacccc caccggtgcg
     5701 gtgcgcgagg tggcgggcgc cgccggccgc gtcctcgtcg gggcggcgcg ctccggtgaa
     5761 cccctcaccg acgtccggtt ggccgggtcc gggctcgcgc tgggtggtgg tgccggcgct
     5821 gctgccgtgc ccgtccggtt ggccgacgcc ggagtcgctg cggtgctcgt cccgggcagc
     5881 cgcgtcgacg tcgtcaccgt aggggcgcgc accgacgagc cgatcgtgct cgccgccgcg
     5941 gtcaccgtgt tggcggtgct gccggaggac agccgcacgt ccggacggct ggtactcgtc
     6001 gcgacggcgc cgggccaggc cgcgcgggtg gcggccgccg cgctcgccga tcaggtcgcc
     6061 attaccctcc gctagcgtga tcaagggttt caaggacttc gtcctgcgcg gcaacgtcgt
     6121 cgagcttgct gtggcggtcg tcgtcggcgc ggcgttcgga tcgatcgtca ccgccttcac
     6181 cagcggcatc atccagccgt tgatcaacgc ggtcacacca ccgagcagcc caggcttcgg
     6241 catccagctc gtcgggggca aggggagcac ctacatcgac ttcgcggccg tcatcggcgc
     6301 tgcgctcaac ttcctcatcg tcgccgcggt ggtctacttc gtgatcgtct tcccactgac
     6361 cgcgctcaag gagcggcgca agcgcggagt cgatgccggc ccaaccgagc ccaccgacgt
     6421 ggagctgctg accgagatcc gcgacctgct ccgcgagcag tcgcagcggc gaggctgaga
     6481 cccctcagtc gcgatcgtga tgtggcggcc ggttggcgag tagctcgtcg tcggccgccg
     6541 caggggcggg ctcgtcacgc tcatcccgtt cgtccgcggt agtggagggc aggacgtcgc
     6601 cgaacacttc gtcgagcctg cggcgagcct gatccgacaa ttcgtccacc ccaccatcct
     6661 gcactgatgc cacgatggac gtatgggact cacggagaag gccaaggaac tcgccgacgt
     6721 cgcactggag aaggcgggcg tgtactcgga gaaggctgcc gcgaaggccg ccgagctctc
     6781 ggacgtggcg cgggacaagg ccccgggcta cctcgaccgt gccgcggaac tggccggcag
     6841 ggccgtcgac agcgcggcag ccggggtgga caaggccacg ggtggccggt tccacgagca
     6901 gatcgagggc gcgtcgacga aggtgggcga gtcgctcgac cgggctcgcc acaccactgc
     6961 ggcacccacc ggcgtccccg acgaggcccc cgggccggtc atcgtcccgc ccgccccggc
     7021 ggacgtggcg ggacaggcag gagcgccggt cacccccgcg gcgaccaacc ccgaggccac
     7081 caagcccgac gccagggata ccggcggcac ggataccggc agcacgccgg gcccgaagag
     7141 ctgacacacg ccggtcgggg cggccgggcg gcgtcgtcag tcgagcccgg acagctccga
     7201 gatcacgtgg gcgacgagcg gcgtgagcgt ggccatacca tcccggacgg ccgcgcggga
     7261 cggtgcgagg ttcaccacca gagtgctgcc tgacaccccg acgagccccc gggacagccc
     7321 ggcgtcgacc gctccggcgg cgagcccgga cgcccggatg gcctcggcga tgccggggat
     7381 cgggcggtcg agcacgcccg ccgtggcgtc ggcggtgaca tcgcgcggag acacaccggt
     7441 gccgccgacg gtgaccacga gatcgacccc gccgatcacg gcagtgttga gcgcgttgcg
     7501 gatcgccacg gggtcgccgg gcaccacgac aacggcgtcg acgaccagct tcgcctcctc
     7561 gagcagttcg gtgaccagcg gcccgatggc atcctcacgt tcaccgtggc tcactcggtc
     7621 gtccacgacc accacgaggg ctcgtccgat ctggggcggc gccatctcca tgcgctcacc
     7681 gtagcgggac gagcacccgc gctccggcac cccgtcctct ttcagaccgg gaacggtctg
     7741 ctgtcgcggg gcgctgtccc atgcccgggc gcccagccgc cggaagcgat cctgatcacg
     7801 ggcgtggcgg ggttcgtcgg ctcacctgac cgggcccggc cggtcagtgg tcggggtcga
     7861 cgccagtggc aggagcacct cgaaacggca gccggggccg gcgttgtggg cgtgaatgtc
     7921 gccgtcgtgc gcttcgacca gcgcgcgagc gatcgccagc ccgaggccac cacccgagcg
     7981 ctcctcgggc gtgcgggccg tgacaccgcg gaacgcgaca tcgaagacgc gctgcaggtc
     8041 gtcctcgggg atgccgccgc aggcgtcgtc gacgcgcagc caggccttgt cgccggtgac
     8101 cccggccgcg acgacgacgg caccggctgg cggcgtgtgc cggatggcgt tggacagcag
     8161 gttgcgcacc acccgcgcga gctcggggtc gctgcccagc accaccggcc agtcgccatg
     8221 ctcctcggcc cgcaccacga cgcccttgcg ggaggccgcc accccctcca cggccacggc
     8281 ctcggacacc acctcgcgca gcggcaccgc ggacagcgtc aggtgcaacg cgccggaggt
     8341 gatccgcgac agctggaaca ggtcgtcgac catccccgac aggcgcacgg tctcaccccg
     8401 gatccggcgg gcgtagtcgg tcacctcgtc ggcgtcggcc acgatcccgt cggagatcgc
     8461 ctcgctcatc gcgcggatcc cggcgagggg ggtgcgcagg tcgtggctga tccaggtcac
     8521 cagctcgcgg cgggacgcct cggcagcacg ttcggcatca cgggcctcgt gcagccacat
     8581 gacgtcactg gcgatgcccc gcccgagcat catcgccgcg ggcaccgtga cgatcgcgac
     8641 ggccccgcac gccacgacgg ccccggcgag ctgcggggtg tacatgaacc cgctgacgcc
     8701 gatcacaccg accatcgtgg cgatcaccgg caccagcacc agcacggtga ccgcagccat
     8761 gatcgagcgt cgccgcatcc ggtgcagcag caccgcgccg agcgcggcga tcggcacgga
     8821 gaacgccagt gccaccggca ccaccgcgtg cagctcctcc ctcacggcga ctgagatccg
     8881 tcgtagcggt agccgacccc ccagaccgtg gcgatgcgcg ccggcttcgt cggatcggcc
     8941 tcgaccttct cccgcagcct gcgcacgtgg accgtgacgg tggactggtc gccgaagtcc
     9001 cagccccaga ccagctccag cagctccgcc cgggtgaaca cctgcccggg gcggcgcgcc
     9061 aggaacgcca gcaggtcgaa ctcgcgcacg gtgagcgcca gggcccgccc ggccagcgac
     9121 gcacgcctgc cgagcacgtc gacacgcagg tcgccgtcga cgaccggtgc ggtggcggcg
     9181 tcacggccgg gcacctcccg ggatcgtcgc agcaccgacg ccacccgcag taccagctcg
     9241 cgcgggctga acggcttcgt cacgtagtcg tcggctccca gctcgaggcc gagcacccgg
     9301 tcctcctcct caccgagcgc ggtgagcatc acgatcggca cgccggtcgt ctcacggcgc
     9361 agccgcctgc acacctccag gccgccaact cggggcagca tgaggtcgag gatgacgatg
     9421 tcggggacct gcccggccac cgcgcgcagc gcctgctcgc cgtcaccggc cacctcgacc
     9481 cggtagcccg agcgatccag gtaacgtgtg acgacctccc gcaccgtcac gtcgtcgtcg
     9541 acgaccaggg ccagtggccg gtccgtcatc gcagtcctcg cacgccgtcg aggctaaccg
     9601 cgcccgttcc aggccgatgg tggctcggat gacgtcaccg atccgtaagc gcgaagctcc
     9661 cggcctcgcg atgccggcct ggcctgttca ccgtcgattt cagcctggtc tggtcggtag
     9721 gtgaaccgcg ccggcgctgg gagggggtgc cggcgccgac gcgatccaga cggtccgttc
     9781 cgcggggcac cgcaggcggg acctcaggaa cggaatcatc tccggaccgc ctccgtcaac
     9841 cactcttggc acatgagcgg tcactgtcag gatgccgcgc cgagcgtcac tgaaacggac
     9901 cggtcggcca gttggacctc gacgacctgc ccgggcgccc gggaccgcac cgccgcctgg
     9961 aggccgtcgc ccgccaggat gcgctggccg tcgaaaccga tcacgaggtc gcccgcccgg
    10021 atccccgcgg cctcggcggc gctaccgggc gccacgctct ggatgcgcgc tcccgagacc
    10081 tgggggtcgt cccccacgct caccccgagg acggcgcggg acgccttgcc ggtgcgttcc
    10141 agttcctcgg ccacgcgcct ggcctggttg atcgggatgg agaagcccac gccgatggaa
    10201 ccgccctgct gacccgtcgt ggcaattgcc gagttgatcc cgatgacccg gccctgcatg
    10261 tccaccagcg ggccacctga gttaccgggg ttgatcgggg cgtcggtctg cagcgcgttg
    10321 agcaccgtgg cctcgctggc cccctggtcc ccgcccacgc ttaccggacg gttcagcgcg
    10381 ctgatgatcc cggtcgtcac ggtgccgccc agccccagcg gcgcaccgaa cgccacgacc
    10441 tgctggccga cgcgtacgga gtcggagttg ccgagctcga tcggggtcaa cctggagacg
    10501 ccctgcgccc gaatcaccgc gaggtccgag ctggggtcgc ggccgacgat cgccgcctgc
    10561 gcggtgtcac cgttctgaaa gacggcggtc acacggcccc ggttcgcggc ggcctcgacc
    10621 acgtggttgt tggtgagcag caggccatcg gcgctcagca ccatcgccga gccctcgccg
    10681 gacgaaccgc cactgtccac gcgcaactgc accacacccg ggagcacgcg ctcggccaca
    10741 gcctccacgg gactcatcgg cgccgtcccg tcgatcggcg ggagcgggct gccgagcact
    10801 ccgttggacg cgccaccgtc tctgctctcg gcgagagagt aacccagcgc tccgccggca
    10861 ccgccggcca gcagcgcccc gaccaggccg aaggccgcca tgcgcgccga tcgcctccgt
    10921 cctggcggcg gggtaaccgg cccgggccac ggggcgttcg gctgggggta ctgcgcggtg
    10981 ccgtacgagc cggggtacgg gccaccggga taggagaacc cgccaccgtg actgctggcc
    11041 tcgcccggcg tcacccgctc gttcggcccg gtaggcgtcg cgtcgcggtc gggggtctcg
    11101 ttcgccatgc catcaccgtg tccgagttcc gggaacctga ctaccgagcc taggaggact
    11161 tgggccctcc cgccgaccgg gtgactggct cagaaccgcg gttgacggcc cggcagccgc
    11221 accgccagca acgcaccgcc ctccggcgcc cggcccgccc acaccgcgcc gccgtgccgc
    11281 accgccacct ggcggacgat cgaaagcccc agcccggagc ccggcatcgt gcgggatgtg
    11341 gccgagcggt agaagcggtc gaagacgtgg ggcaggtcct cctcggcgat gccaggcccc
    11401 gcatcggcca cctcgagcac gatcgaccac tcgtcgaggg ggcgcatctg cacccgggcc
    11461 gtgcctcccg gcgggctcca tttcgccgcg ttgtcgagca ggttgagcac ggcgcgctcg
    11521 agcgccgtcg agtctccgac gagcgtccac ggcacgagca tcggcacgaa ctcgacggcg
    11581 ccggcccgcc gccgggcccg ttcgagcgag cgcagcacca cgtcggtcag ctcgagcggc
    11641 tcgtgcacca cgagtggtac gtcgtcgcgg gccaactcga cgagatcccc gaccagcgtg
    11701 gacagctccg tcacctgcgc ctgcacgtct tcgaggatct ctgcgcgatc ggactccggc
    11761 agcatcggag caccgggcct gttggcggcc gcgagcagct ccaggttggt gcgcatcgac
    11821 gtcagcggcg tgcgcaactc gtgcccggcg tcggcgacga ggcggcgctg ttgctcctgg
    11881 gaggcggcga gcgcaccgag catgtcgttg aagctgtggg tgagccgggc cagctcgtcg
    11941 gaaccgtcga cgggaatggg ccgcaggtcg ttcgtggcgg cgacccgctc cgtggcggcg
    12001 gtgagccggt ccaccgggcg cagcccggcc ctggcgacga cgaccccggc gagcgcggcg
    12061 agcaccaccc cgatcccgcc gacgatcggc agggcgactc cgagcttggt gagcacctgc
    12121 tgggtcgagt cgagccgctg cgcgatcacc agcgcctgcc cgggtccggc ctgcacggcc
    12181 acgacccggt actggacgcc gtcctcggcg gctgtccgca cggaggacgt ctcgatgccc
    12241 tgggcaacct ccagctcgtc ggcgccgagc gggggagccg atgcctggcc ctcggcagag
    12301 ctcgcccggc cgttggccag gaggagggcg agccggatgt cgcccgcgcc gagcaccccg
    12361 gtggggatgg tggccagctg ccgcgggtcg gccagctcgc tctgggcggc ggcggacgcg
    12421 cgctgcagga gattggcgtc gagggtctgc atgatgttga cgcgcacggt cacgaacgcg
    12481 gcgacggaca ccagaatcac cgcgagcgcg gccaccagcg cggccagtac cccgatgcgg
    12541 gcacgcagcg agacgcggtc gaacgcctcg atccgaggag tggagccgtc cgggcggccc
    12601 tgccgtacgc gttccgccgg cccgttcccc ggcgtcgcat cgtcgttcgt ggcatcgccg
    12661 ggcgtggccg catgcgcgcc cacggcatcg ctgcccggac ccgcacgctc gctcacggcg
    12721 gcgtgtcgcg cagcacgtat ccgaccccgc gaacggtgtg gatcacccgg ggttcaccct
    12781 cggcctcggt cttgcggcgc aggtagccga tgtacacctc gagtgcgttc ccggtggtcg
    12841 ggaagtcgta gccccacacc tgctcgagga tctgggcgcg ggtgagcacc cggcgggggt
    12901 gggcgagcaa cagctccagg agcgagaact cagtgcgggt gagactgatc ggccgatccc
    12961 cccgacggac gtcgcgagtg tcgggatcca ggctgaggtc ccagaactcc agcggcttgc
    13021 tctggcccgc cgctgcggcg atctcgtcgg gcgtccggcg ccgcagcagc gcgcgcagcc
    13081 gcgcgagcag ctcctcgagc gcgaacggct tgggcaggta gtcgtcggcg cctgcgtcga
    13141 gcccggccac ccggtcggag acggcatccc gcgcggtgag cacgaggatc ggcaggtcgt
    13201 caccgccacc ccgcagccgt cggcagacct ccaggccgtc caggcgcggc atcatcacgt
    13261 cgagcaccat cgcgtcgggc cgctgtatga ggacggcgtc gagcgcggcc tgcccgtcgt
    13321 tcgcgagctc gacctggtag ccgttgaacg cgagcgagcg gcgcaacgag tcgcggacgg
    13381 accggtcgtc gtcgacaacg aggatgcgca tgcgaccagt gtgacctgcg catctgagac
    13441 gcgcctgaga ggtacttgtg cagagcacca tgtgttgcga caccgcgagg tgcctgtccg
    13501 acttcgtggt tcggccgctc agcagctccc ggctgagcgc aacttcgccg acgtgtcacc
    13561 cgcgggtgcg atggcggtag agccaggtcg agccggtgaa cgtgaccacc aggatgccga
    13621 ccgtccacgc gagcgcaacc catcccctgt cgccgagcgg cgtgccggtg agcaagccgc
    13681 ggaccgtctc gatgacgggg gtgatcggct ggtgctcgct gatcccctgc aggaacgacg
    13741 gcatggtgtc ggtcggcacg aacgcgctgc tgaggtaggg aacgaacagc atgaagaagc
    13801 tcagcacgct tgccgactcc acgctcctcg cgatcacccc gagacctgcg gcgagccacg
    13861 acatcgcgag cacgtagagc agcaacaggc cggccgcgag cgcccagtcg ctgagcgtcg
    13921 cggttggccg gaagcccatc gcgatcgccg cgacgatgac gatcgtggtg gcgagggcgt
    13981 tgcgggccac gctcgcggtc acgtgcccgg tgaggacggc ggagctgcgg atgggcagcg
    14041 accgcagccg gtcgatcatg ccgctggtca tgtcactggc cacggacatc gcggtggacg
    14101 acgccccgta gccggtgcac agcaggacga tccccggcac cacgtagttg atg
//
LOCUS       DASRSC010000146        16364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_46514,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000146 DASRSC010000000
VERSION     DASRSC010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 16364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16364
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46514"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1296)
                     /locus_tag="VFE39_07045"
     CDS             complement(76..1296)
                     /locus_tag="VFE39_07045"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET6258390.1"
                     /translation="MYRVDSEVGQLRQVILHRPGLELKRLTPGNKDRLLFDDVLWVAR
                     AQEEHDRFAALLRARGITVHLFGELLRTTVEIPEARKYILDRIFDDRVYGPLAIDALR
                     NCFDAMDVDALTDHLIGGITKREVLERIQEPRSIAFHVLAADDFVLEPLPNHLYTRDT
                     SAWIHGGVAINSMRKKARMRETVHYEAVYRWHPLFTRSDYALWSEGSVNGPATTEGGD
                     MHVLGGGAVLIGLSERTTPAGVERLATRLFAGGEIDRIVALRMPQQRAFMHLDTVMTM
                     VDPETFVEYAGLGKLPAYTVRPGDSEKELDVTDHPPEEMHDAIAAALGLSSVRVLTPE
                     QDVHAAAREQWDDGCNALAVAPGVVVTYERNVTTNRHLRRHGIEVLDIPGGELGRGRG
                     GPRCMSCPIVRDAA"
     gene            1340..1810
                     /locus_tag="VFE39_07050"
     CDS             1340..1810
                     /locus_tag="VFE39_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET6258391.1"
                     /translation="MQDVRVQLDRLDEDLIALLVDDARRSYADLGASVGLSASAVKRR
                     VDRLRAGGAITGFTVRLDPGALGWSVEGYVELYCRNRTAPATIRATVARFPEVVDAST
                     VSGEADAVLRILAADMRHFEQVLEQIGAQPFVARTKSVLVLSPLLRRPAAPLPH"
     gene            2123..2911
                     /locus_tag="VFE39_07055"
     CDS             2123..2911
                     /locus_tag="VFE39_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidinotransferase"
                     /protein_id="HET6258392.1"
                     /translation="MCRPEHFCVEYAINPWMHPGVPVDRGRALAQWEGLRQTYLGLGH
                     RVDLLDPVPGLPDMVYAANGATVVDGVVLGARFRHRERAAEAGAHRRWFRAAGFTRVV
                     EPEFMNEGEGDLLVVGPPGDTVVLAGSGFRTDPRAHAEAAAVLGRPVVSLELVDPCYY
                     HLDTAIAVLDDATIAWLPEAFAPASQAVLRERFPDALVADPADAMVLGLNAVSDGRHV
                     VLPAPATRLAAAVAERGFTPVPVEMSELLKGGGGPKCCTLEVRA"
     gene            2974..4161
                     /gene="rocD"
                     /locus_tag="VFE39_07060"
     CDS             2974..4161
                     /gene="rocD"
                     /locus_tag="VFE39_07060"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HET6258393.1"
                     /translation="MHEHSAHNYHPLPVVVAQAAGAWVTDVEGRRYLDCLAGYSALNF
                     GHGHPALVAAAHDQLDRVTLTSRAFGSDQIGPFCAELAALVGKQMVLPMNTGAEAVES
                     GLKVARKWGYEVKGVPEDRARIIVAAGGFHGRTISIVSFSTDPDARGGFGPFTPGFDV
                     VPYGDPDALAAAITPDTVAVLIEPVQGEAGVVVPPAGYLAAVREICDATGVLFVADEV
                     QSGLGRTGHVLATRAADVDADVYLLGKALGGGIVPLSAVVADAAVLGVLRPGQHGSTF
                     GGNPLACAVGRAVLELLSDSGPHGMLARAARLGEVLQNGLAALVGSGVVAVRRSGLWA
                     GVDVDPRLGTGREVSERLVRRGVLVKDTHGSTIRLSPPLVITPDELVSAVDALAGVLA
                     ELG"
     gene            complement(4223..4915)
                     /locus_tag="VFE39_07065"
     CDS             complement(4223..4915)
                     /locus_tag="VFE39_07065"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6258394.1"
                     /translation="MPPIDPPRPDDPRARAARTKRYRTRRALLDAADATFGSRAWASV
                     RMEDIAAGAGVSVATAYNHFPSKHALIGHLYSPLIQPLLVQAQRDIAEHRPVVDALCD
                     QVKALTRLTFRYPTLTAAFWSAVQEYTIRVAGPPDPTDDIDPRTLAPIPEAIRVLVEH
                     GQGTGEVRRFPSAVEVSALIVNTLLIRSVNRPHEPPEITSEMLLTVMFGMLRPDLLAD
                     TGPDGRPFRCRV"
     gene            5452..6039
                     /gene="shbA"
                     /locus_tag="VFE39_07070"
     CDS             5452..6039
                     /gene="shbA"
                     /locus_tag="VFE39_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor ShbA"
                     /protein_id="HET6258395.1"
                     /translation="MPEPSLLDGLVAEALAGEPRAKDRLLGTIHPLVLRYCRGRLGRQ
                     ETVIGSADDIAQEVCLAVVNALPGYTIKGLSFRAFVYGIAAHKVTDAFRAISRNRAEP
                     MADLPDFPVVLDGPEHHLLATELTERLSGLLRHLTPRQREVLVLRIAVGLSAEETAQA
                     VGSTPGAVRVTQHRALNRLRRILAGYFVEDEPDEE"
     gene            6135..6545
                     /locus_tag="VFE39_07075"
     CDS             6135..6545
                     /locus_tag="VFE39_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET6258396.1"
                     /translation="MSAGQIAALIAAGAFVVLVLLLAVPLLKLGRTLDEATVAIRKAH
                     EGSAPLLNDAQTTLHQVNTQLARVDGITSSARAVTSNVSVLTSLFTATLGGPLVRAAA
                     FSYGLNRAVKARRAGRDAGAHSKRSRPGRRRGRR"
     gene            6542..6853
                     /locus_tag="VFE39_07080"
     CDS             6542..6853
                     /locus_tag="VFE39_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258397.1"
                     /translation="MKRLFWLGLGLAAGAYATRRASAAAHSLTPAGVGANIADGLREL
                     GAGLGAFGAEVRAGMVERERELTELVERRTGGVLPALGDAFVESQPMVRPGARARRAG
                     E"
     gene            6951..9611
                     /gene="alaS"
                     /locus_tag="VFE39_07085"
     CDS             6951..9611
                     /gene="alaS"
                     /locus_tag="VFE39_07085"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET6258398.1"
                     /translation="MQTHEITRRFTDYFTAAGHISVPSASLILDDPNLLFVNAGMVQF
                     KPYFLGDVPPPYARATSIQKCVRTGDIDEVGRTTRHNTFFQMAGNFSFGDYFKAGAIE
                     HAWSLITSSQDAGGYGFDPDRIWVTVFRDDDEAVDLWRRIAGLPLERIQRRGGKDNYW
                     DMGVPGPGGPSSEIYYDRGPEHGREGGPEADEDRYLEIWNLVFMQDVRGELSPKDGHP
                     PVGVLPHKNIDTGMGVERVAYLLQGVDNVYETDLVRPVIARAEEFSGRRYGADHADDV
                     RFRVIADHARSGVMIIGDGVTPSNEGRGYVLRRLLRRIVRSARLLGVNEPVLNSFAEV
                     VRDEMAPTYPELVTDFERISAVVQAEEEAFLATLSAGSRIFDTAVAETKRGGGLVLPG
                     DRAFQLHDTYGFPIDLTLEMAAEAGLEVDQDRFASLMNEQRTRAKADAATRKVGHGDG
                     SIYRSVLDTHGATDFLGYTDLSAEATIVGLLVDGVPVAAAREGEQVEVVLDRTPFYAE
                     AGGQQADTGELRGASFTVRVDDVQSPVAGLRVHRGTVIAGEVSLDAAVVAEVDRTRRA
                     SVSRAHTATHLVHAGVRGALGTAAAQAGSLNAPGRLRFDFTSPTGAVRPAALAEIEDE
                     VNTVLQQNREVESYETTMDEARRLGAMMLFGEKYGDRVRVVDMGDYSRELCGGTHVGR
                     TGEIGMIKVLSEASIGSGVRRVEALVGLDAFRHLSKEHLLVSTLADQLKSRPEDLPDR
                     INGLVERLRTAERDLEKVRADAVLSSAGALAAGAEDIDGVALVAVAAPDGVTGNDLRA
                     LASDVRGRLGSRPGVVALFSGDGDKVSFVVATTAAARDRGLAAGGLIPAFAPAVGGRG
                     GGKPDLAQGGGTNPTGIPAAVTALRSALRG"
     gene            9622..10047
                     /gene="ruvX"
                     /locus_tag="VFE39_07090"
     CDS             9622..10047
                     /gene="ruvX"
                     /locus_tag="VFE39_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET6258399.1"
                     /translation="MRGRRLGVDVGAVRIGVAVSDPAGVLATPLVTVPRDLEDGTDLR
                     TIADLVTEYDVVGVVVGLPRTLAGREGAAAETARAFAVALEEAVDVPVELSDERLTTV
                     VATRQLRERGVKGRKQRAVVDQAAAVAILQGWLDAARRR"
     gene            10260..11357
                     /locus_tag="VFE39_07095"
     CDS             10260..11357
                     /locus_tag="VFE39_07095"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET6258400.1"
                     /translation="MLLLAVLLLLGGVAGGGYYVYSSVFATPDFEGPGSGDVIVQVHD
                     GDSTTQIGSTFAQRGVVAGVQAFTEAAAEEPRIRSVQPGYYQMRLRMSGAAAVAQLLE
                     PSARVGQLEIRGGVQLDDTRASDGTVTPGVLSLISKATCIEQNGTEQCLPVDQLRAAM
                     MDTDPAALGVPAWALEEVGRAEPARRLEGLLMPGRYDVAPGTPAVDVLRGLLATSDAR
                     LEAAGLVAGAQSIGSTPYHVLTIASLVEKEAITPDMPKVARVIYNRLGAGQRLELDSM
                     VNYPLDLQALRTTADDRARPGPYNSYVVAGLPPTPISAPGSAAIEAALTPAPGPWFFF
                     VRCQTDGTSCFATTFAQHATNVQEARANGAF"
     gene            complement(11381..12247)
                     /locus_tag="VFE39_07100"
     CDS             complement(11381..12247)
                     /locus_tag="VFE39_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HET6258401.1"
                     /translation="MCGRAERAARTTVRAEVDHGHAELLGDPDRPQGWTLLLDGTAQS
                     HVDLDDPTHLEFEYVRRLGHVVDLMAPPAAPLRVLHLGGGAWTLARYVATTRPRSTQQ
                     VVELDAELVELVASRLPADGLGIEVTVADARAALARVAPRSVDLLVLDAFAGARTPAH
                     LTSTEFLDSAADTLTPAGVYAANVADGGELSFARSQVAAALARFAEVAVLASPEILHG
                     RRFGNLVLLGSAAPLPVDVLVRRTAADPFPARVLTGEDVGRFAGAARGPSDRTAIPSP
                     QPPPGFFGRPAS"
     gene            12645..13427
                     /locus_tag="VFE39_07105"
     CDS             12645..13427
                     /locus_tag="VFE39_07105"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HET6258402.1"
                     /translation="MRDRDRARGAAAVGRPVVLVAVLGAMLTVGLPLALVGAGGPVAV
                     VAAGICGGVGGGAAGAAGRVLLGRLRRGAPIRPPWCELAVGALWAGTVPAWAAGVLPT
                     AWVPALLGLSWLGVVAGAVDVTHRRLPDALTLPALPLALVFLLPLGADAVLRGAAGAA
                     VAAGAHAAVHLLACGAMGAGDVKLAAPLGAVLAAAGWPALVLAGLLAAVVTAALGATG
                     LATRRLRRGAALPHGPSMLVAAWLVTSWLVATGAVTGHTGGE"
     gene            13441..14613
                     /gene="aroC"
                     /locus_tag="VFE39_07110"
     CDS             13441..14613
                     /gene="aroC"
                     /locus_tag="VFE39_07110"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET6258403.1"
                     /translation="MLRWITAGESHGPALVAVLEGMVAGVEITTKELASELARRRLGY
                     GRGARMAFEADELEVIGGVRHGVTQGGPVAVRIGNTEWPKWETVMAADPVDPELVAGR
                     ARNAPLTRPRPGHADMAGMTKYGFEDARPVLERASARETAARVVLGTVAKAFLKQAFD
                     VDIVSHVVALGSVEAPDDEQPGPGDLERVDASPVRAYSEAATHAMVAEVDAAQRDGDT
                     LGGVVEVIAFGLPVGLGSYVQSDRRLDARLAGALMGIQAMKGVEIGDGFRTARRRGSA
                     AHDEMIPGDPVKRLSNRAGGIEGGMSNGEPVRVRVAMKPISTVPRALQTVDVATGEAA
                     QAIHQRSDVCAVPAAGVVAEAMVALVLADAALEKFGGDSLSETCRNFRAYRDTGAH"
     gene            complement(14894..15151)
                     /locus_tag="VFE39_07115"
     CDS             complement(14894..15151)
                     /locus_tag="VFE39_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258404.1"
                     /translation="MLRTADDPPPPAREQLPLPRRRQQAHLEPQLRNPGDAGTGTPFA
                     AFAGESSNADDAPADDTSAETDLAAAFLAATRRRRRYRFGD"
     gene            15452..16072
                     /locus_tag="VFE39_07120"
     CDS             15452..16072
                     /locus_tag="VFE39_07120"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET6258405.1"
                     /translation="MSLERRPALVIVGAPGAGKTTVGRVLARRLGVEFTDVDALIVER
                     AGKAVADIFLQDGEDVFRAMEREVVAEALTGTDGVLALGGGSVLAAETRMRLRGHRVV
                     HLVVGLADGLRRTGMSTARPLLAGVNPRATFKALLDARAPLYREVATVEIDTTRRSAN
                     QVARSVLEAVGEVLPPAAEEPVDPDDPLDRPTPHDSALLPDPSLRP"
     gene            complement(16156..>16364)
                     /locus_tag="VFE39_07125"
     CDS             complement(16156..>16364)
                     /locus_tag="VFE39_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258406.1"
                     /translation="PRWRSLASPASSPSPPPASATTIPTWRSVQDGTTPEGGWKLTRT
                     DLARFITDELGRHRWSRAAPTIAH"
BASE COUNT         2172 a         5733 c         6108 g         2351 t
ORIGIN      
        1 gacgcatgat cgccggccgg gcgcgttccg cggccggatc cgaccgttcc cgcgtcgagc
       61 cggtgatcac cgtgctcacg ctgcgtcccg cacgatcggg cagctcatgc agcgggggcc
      121 gccgcgcccg cgtcccagct cgccgccggg gatgtcgagc acctcgatgc catggcggcg
      181 cagatgcctg ttcgtcgtga cgttgcgctc gtacgtcacc acgaccccgg gcgccaccgc
      241 gagcgcgttg cacccgtcgt cccactgctc gcgggcggcg gcgtgcacgt cctgctcggg
      301 ggtcagcacc cggaccgacg agagcccgag tgcggccgcg atcgcgtcgt gcatctcctc
      361 aggcgggtgg tcggtgacgt cgagctcctt ctccgagtcg ccgggacgca cggtgtacgc
      421 aggcagcttg ccgagccccg cgtactccac gaacgtctcc ggatcgacca tcgtcatcac
      481 cgtgtccagg tgcatgaatg cccgctgctg cggcatccgc agcgcgacga tccggtcgat
      541 ctccccgccg gcgaacaggc gcgtggccag ccgctcgacc ccggcggggg tggtccgctc
      601 cgagagcccg atcaacaccg ccccgccgcc gagcacgtgc atgtcgccgc cctcggtggt
      661 cgcggggccg ttcaccgagc cctccgacca gagcgcgtag tcggagcggg tgaacagcgg
      721 gtgccaccgg tacacggcct cgtagtgcac ggtctcgcgc atcctggcct tcttgcgcat
      781 gctgttgatc gccaccccgc cgtggatcca ggccgacgtg tcgcgggtgt agaggtggtt
      841 ggggagcggc tcgagcacga agtcgtcggc ggcgagcacg tggaaggcga tcgagcgcgg
      901 ctcctggatc cgctcgagca cctcgcgctt ggtgatcccg ccgatgaggt gatcggtgag
      961 agcgtcgacg tccatcgcgt cgaagcagtt gcgcagcgcg tcgatcgcga gggggccgta
     1021 cacgcggtcg tcgaagatcc ggtcgaggat gtacttgcgc gcctcgggga tctccaccgt
     1081 cgtgcgcagc agctccccga acaggtgcac cgtgatcccg cgcgcccgca gcagcgccgc
     1141 gaaccggtcg tgctcctcct gcgccctggc cacccacagc acgtcgtcga acagcagacg
     1201 gtccttgttg ccgggcgtca gtcgcttcag ctccaacccc ggacggtgca ggatcacctg
     1261 gcgcagttgc ccgacctcgg agtccacgcg gtacatgcgc tcatcctgca cgtgccccgc
     1321 gcccgcgtcc cgtggcgacg tgcaggatgt gcgcgtgcaa ctcgaccggc tcgacgagga
     1381 cctcatcgcg ctgctcgtgg acgacgcgcg ccgcagctat gccgacctcg gcgcgagcgt
     1441 cgggctctcg gcatcggcgg tgaagcggcg cgtggaccgg ctccgcgcgg gtggggcgat
     1501 caccggattc accgtgcggc tcgatccggg ggcgctgggc tggtcggtgg agggctacgt
     1561 ggagctctac tgccgcaacc gcaccgcgcc cgccacgatc cgggccaccg tcgcgcggtt
     1621 ccccgaggtg gtcgacgcca gcacggtttc gggggaagcc gacgccgtgc tgcgcatcct
     1681 cgccgccgac atgcgccact tcgagcaggt gctcgagcag atcggggcac agccgttcgt
     1741 ggcgcgcacc aagtcggtgc tcgtgctctc gccgttgctc cgccgccccg cggcgcctct
     1801 cccacactga cccggggctg cttcatcacg acggccgcgc gcgagcgcct ccggccgggc
     1861 ggcggtgtgc ggctcgtggc gatcggacct gcggcgatcg tccgcgcaac ggatgagcac
     1921 ccgaggccgc cgacgcgcac gaatccgctg cgaagaggca atgcttcgca attgtccgcc
     1981 gcacatgccg ttcctaatct gatgaacgtc ccggtcccgt cgctgcggcc accctgctac
     2041 ctcgtggagc gagcccgtga cgagcgtcct gtcccgcccg ggcgagcgca ccgagcgcac
     2101 cgcctcccgc cgccgctacc tcatgtgccg gccggagcac ttctgtgtcg agtacgcgat
     2161 caacccgtgg atgcatcccg gggttcccgt cgaccggggc cgggcgctgg cccagtggga
     2221 ggggctgcgc cagacctacc tcggcctcgg ccatcgcgtc gacctgctcg accccgtccc
     2281 ggggctcccc gacatggtct acgcggcgaa cggcgccacc gtcgtcgacg gggtcgtgct
     2341 gggtgcccgg ttccggcatc gggagcgggc cgcggaggca ggcgcgcacc ggaggtggtt
     2401 ccgcgcggcc ggcttcaccc gtgtcgtcga gccggagttc atgaacgagg gggagggcga
     2461 cctgctcgtc gttggtcctc ccggagacac ggtggtgctg gccggatccg gtttccgcac
     2521 cgacccgcgc gcccacgccg aggcggccgc cgtgctgggc cggccggtgg tgtcgctgga
     2581 gctggtcgac ccctgctact accacctcga cacggcgatc gccgtgctcg acgacgcgac
     2641 gatcgcgtgg ttgcccgagg ccttcgcgcc cgcgtcgcag gcggtgctgc gcgagcgttt
     2701 ccccgacgcg ctcgtcgccg acccggccga cgcgatggtg ctggggctca acgccgtcag
     2761 cgacgggcgt cacgtcgtcc taccggcacc ggccacccgg ctcgccgccg ccgtggccga
     2821 gcgcggattc acgcccgtcc ccgtcgagat gtccgagctg ctcaagggcg gcggtggccc
     2881 caagtgctgc acgctggagg tgcgcgcatg acgatcatcg aatccggccc cggcgctgcc
     2941 gtcggcacgg cgaccgcgca ccacgagcac ctcatgcatg agcattcggc gcacaactac
     3001 catccgctgc cggtagtggt cgcgcaggcc gcaggcgcat gggtcaccga cgtcgagggc
     3061 cgccggtacc tcgactgcct cgcgggctac tcggcgctga acttcgggca cggccacccc
     3121 gcgctcgtcg cggcggccca cgaccagctc gaccgcgtga cgctcaccag ccgcgccttc
     3181 ggcagcgacc agatcgggcc gttctgcgca gagctcgccg cgctcgtcgg caagcagatg
     3241 gtgctgccga tgaacaccgg ggcggaggct gtcgagagcg ggctcaaggt cgcgcgcaag
     3301 tggggctacg aggtgaaggg cgtgccggag gaccgcgccc gcatcatcgt ggcggccggt
     3361 gggttccacg gccgcacgat cagcatcgtc agcttctcca cggaccccga cgcgcggggc
     3421 gggttcgggc cgttcacccc gggcttcgat gtcgtgccct acggcgatcc cgacgcgctg
     3481 gccgcagcga tcacgcccga cacggtcgcg gtgctgatcg agcccgtgca gggcgaggcc
     3541 ggcgtggtgg tcccgcctgc ggggtacctg gcggcggtcc gcgagatctg cgatgccacg
     3601 ggcgtgctct tcgtcgccga cgaggtgcag tccggcctcg gccgcaccgg acatgtgctg
     3661 gccacccggg ccgcggacgt cgatgcagac gtgtacctgc tcgggaaggc cctcgggggc
     3721 gggatcgtgc ccctgtccgc ggtggtcgcc gatgccgcgg tgctcggcgt cctgcggccg
     3781 gggcagcacg gcagcacgtt cggcggcaac ccgctcgcct gcgccgtcgg ccgcgccgtg
     3841 ctcgagctgc tgagcgacag cggcccgcac gggatgctcg ccagggcggc tcggctcggc
     3901 gaggtcctgc agaacgggct tgcggcactc gtcggtagcg gcgtggtggc ggtgcggcgg
     3961 agcggcctgt gggccggcgt cgacgtcgac ccccggctcg gtaccgggcg ggaggtcagc
     4021 gagcgactgg tccggcgcgg ggtgctcgtg aaggacacgc acggctcgac gatccggctc
     4081 tcgccgccgt tggtgatcac ccccgacgag ctggtgtcgg ctgtcgacgc gctggcgggg
     4141 gtgctggccg agctgggctg agacgaggtg gtcggcgctc gatcggaccc ggaaacggtg
     4201 gcccggtgac gggtcggagc gctcagaccc gacagcggaa cggcctcccg tccggtccgg
     4261 tgtccgcgag caggtcgggg cggagcatcc cgaacatcac ggtcagcaac atctcggacg
     4321 tgatctcggg cggctcgtgg ggccggttga cgctccggat caacagcgtg ttcacgatca
     4381 aggcgctcac ttccacggcg ctggggaacc ggcggacctc gccggtgccc tgcccgtgct
     4441 cgacgagcac ccggatcgcc tcggggatgg gtgcgagggt gcgggggtcg atgtcgtcgg
     4501 tcgggtcggg cggcccggcc accctgatcg tgtactcctg caccgcggac cagaacgccg
     4561 cagtgagcgt cggatagcgg aaagtgagcc gcgtcagggc cttgacctgg tcgcacagcg
     4621 cgtcgaccac cggtcggtgc tccgcgatgt cgcgctgggc ctgcaccagc agcggctgga
     4681 tcagcgggct gtagaggtgt ccgatgagcg cgtgcttgga ggggaagtgg ttgtaggcgg
     4741 tggccacgct gacgcccgct ccagctgcga tgtcctccat ccggacgctc gcccaggcac
     4801 gggagccgaa cgtagcgtcg gcagcgtcga gtagagcccg cctcgtccgg taacgcttcg
     4861 tccgtgccgc gcgcgcccgc ggatcgtccg gtcgaggggg gtcgatggga ggcattgatc
     4921 gcagagtaac caaccattgg tcgaagttct cttgactcca acttgggtgt cacccaaact
     4981 gcgtatgtat ttgaacgtag aacgacttcg atcaccctgc tgggcacggc gggtgcgcgg
     5041 aggtactgga gcggcatcga acaaccggcc ctgccgtcgg gggactcgca ggtatctagc
     5101 ggttgtccgg tcgctcctca agcacgacgg cagtaccgcc gccaggccct gaccgagggc
     5161 aggcggcaac cgcgtccgtg ttacgccggc ggaagtcgcc ggtgcagggg aggggaaccc
     5221 taaagtgatg ttcaggcctg agcgccattc tgctgcaact accggtaaac cacgatgagc
     5281 gccctcgcgg cggccgcctt cgcggctgaa ccgccaggcg ttgttccaga ggccggtctc
     5341 cctacgtggg tgcacccggc ggcggactgg acgggacacc ggggggccga gtccatacgc
     5401 gacacacctc cggacggcgt gcgccctgcg gacgaccggg cgcaggacga catgccggag
     5461 ccgtcgctgc tggacgggct ggtggcggag gccctggcgg gagagccgcg ggccaaggac
     5521 aggctgctcg gcacgatcca cccgctggta ctgcgctact gccgagggcg gctcggccgc
     5581 caggagaccg tcatcgggtc ggccgacgac atcgcccagg aggtgtgcct ggccgtcgtc
     5641 aacgcgctgc ccgggtacac gatcaagggc ctgtcgttcc gggcgttcgt ctacggcatc
     5701 gccgcgcaca aggtgacgga cgcgttccgt gccatcagcc gcaaccgtgc ggagccgatg
     5761 gccgacctgc ccgacttccc ggtcgtgctc gacgggccgg agcaccacct gctcgcgacc
     5821 gagctcaccg agcgcctcag cgggctgctg cgtcacctga cgccgcgcca gcgcgaggta
     5881 ttggtgctgc gcatcgccgt cgggctgagt gccgaggaga ccgcgcaggc ggtcggttcg
     5941 acgccgggtg ccgtgcgggt cacccagcat cgtgctctca accggctgcg ccggatcctc
     6001 gccgggtact tcgtggagga cgaaccggac gaggagtgaa tggcgacgca tcgggcctcc
     6061 ggaagaaccg aggtcgtcag ggagtagcct gatcgccgat caactccctg acgggcgggt
     6121 atggaggagg cgtcgtgtcg gctggccaga tcgcggcact gatcgccgcg ggggccttcg
     6181 tggttctcgt gctgctgctg gccgttccgc tgctgaagct cggtcgcacc ctcgacgagg
     6241 ccaccgtcgc catccggaag gcccacgagg gcagcgcacc gctgctgaac gacgcgcaga
     6301 cgaccctgca ccaggtgaac acacagctgg cgcgggtgga cggcatcacg tccagcgccc
     6361 gcgcggtcac gagcaacgtc tccgtgctga cgtcgctgtt cacggctacc ctcggtgggc
     6421 cgctcgtgcg ggccgccgcg ttctcctacg gcctgaacag ggccgtgaag gcccgacggg
     6481 ccggccgcga cgccggggcc cactcgaagc ggagccggcc tggacgccgg aggggcaggc
     6541 ggtgaagagg ctgttctggc tcgggctcgg cctcgctgcg ggggcgtacg ccacccggcg
     6601 ggccagcgct gccgcacaca gcctgacccc cgcaggggtg ggggccaaca tcgccgacgg
     6661 tctgcgtgag ctcggcgcag ggctgggcgc gttcggtgcc gaggtgcgcg cagggatggt
     6721 cgagcgcgag cgcgagctca ccgagctcgt ggagcgtcgc acaggtgggg tgctgcccgc
     6781 actcggcgat gcgttcgtcg agtcgcagcc catggtgcga ccgggcgcgc gagcgcgccg
     6841 ggcaggggag tagtggcgct cctgcgccct cccctgccga tccgcgtctc gcccgtgagg
     6901 gagactgctt gtaggcctcc ctttctcgca caacaccagg aagcaccgcc gtgcagaccc
     6961 acgagatcac tcgtcgcttc accgattact tcaccgccgc cgggcacatc agcgtgccca
     7021 gcgcgtccct gatcctcgac gacccgaacc tgctgttcgt caacgcgggg atggtgcagt
     7081 tcaagccgta cttcctcggt gacgtgccgc cgccctacgc gcgcgccacc tccatccaga
     7141 agtgcgtgcg gaccggcgac atcgacgagg tcggccgcac cacgcggcac aacacgttct
     7201 tccagatggc cgggaacttc tccttcggcg actacttcaa ggcgggcgcg atcgagcacg
     7261 cctggagcct gatcacgagc agccaggacg ccgggggcta cggcttcgac cccgaccgca
     7321 tctgggtcac ggtcttccgc gacgacgacg aggccgtcga cctctggcgg aggatcgccg
     7381 ggctgcccct ggagcgcatc cagcgtcgcg gtggtaagga caactactgg gacatgggcg
     7441 tgcccggccc gggcggcccc tcctccgaga tctactacga ccgcgggccg gagcacggcc
     7501 gcgagggcgg cccagaggcc gacgaggacc ggtacctgga gatctggaac ctcgtcttca
     7561 tgcaggacgt gcgcggcgag ctcagcccga aggacggcca cccgccggtc ggcgtgctgc
     7621 cgcacaagaa catcgacacc ggcatgggtg tcgagcgggt cgcgtacctg ctgcagggcg
     7681 tcgacaacgt gtacgagacc gatctcgtgc gcccggtcat cgcgcgggcc gaggagttct
     7741 cgggccgccg ctacggcgct gaccacgccg acgacgtccg cttccgcgtg atcgccgacc
     7801 acgcacggtc cggagtgatg atcatcggcg acggcgtgac gccgtcgaac gaaggccgcg
     7861 gctacgtgct gcgcaggctg ctgcggcgca tcgtgcgttc ggcgcggctg ctcggcgtga
     7921 acgagccggt gctcaactcg ttcgccgagg tcgtgcgcga cgagatggct ccgacgtacc
     7981 cggagctcgt caccgacttc gagcggatct cggcggtcgt gcaggccgag gaggaggcgt
     8041 tcctcgccac cctcagcgcg ggctcgcgca tcttcgacac cgcggtggcg gagacgaagc
     8101 gcggcggcgg tctcgtgctg cccggtgacc gggcgttcca gctgcacgac acctacggct
     8161 tcccgatcga cctcaccctg gagatggccg ccgaggcggg cctcgaggtc gaccaggacc
     8221 gcttcgcctc gttgatgaac gagcagcgca cccgggccaa ggccgacgct gccactcgca
     8281 aggtcggcca cggcgacggc tcgatctacc gcagcgtgct cgacacccac ggcgccaccg
     8341 acttcctcgg ctacaccgac ctcagcgccg aggccaccat cgtcggcctg ctcgtcgacg
     8401 gcgttccggt ggcggcagcc cgggagggcg agcaggtgga ggtggtgctc gaccgcaccc
     8461 cgttctacgc cgaggccggt ggccagcagg ccgataccgg cgagctgcgg ggagcgtcgt
     8521 tcaccgtgcg ggtcgacgac gtgcagtcgc cggtcgcggg gctgcgggtg catcgtggca
     8581 ccgtcatcgc cggcgaggtg tcgctcgacg ccgccgtcgt ggccgaggtg gaccggacgc
     8641 ggcgggcctc ggtctcgcgg gcccacaccg ccacccacct cgtgcacgcc ggcgtccgcg
     8701 gggcgctggg caccgccgcg gcccaggccg gctcgctcaa cgccccgggc aggctgcggt
     8761 tcgacttcac ctcgcccacc ggcgcggtgc ggccagccgc gctcgccgag atcgaggacg
     8821 aggtcaacac ggtcctgcag cagaaccgtg aggtcgagtc ctacgagacc acgatggacg
     8881 aggccaggcg gctcggcgcg atgatgctgt tcggcgagaa gtacggcgac cgggtccggg
     8941 tggtcgacat gggcgactac tcgcgcgagc tgtgcggtgg cacccacgtc gggcgcaccg
     9001 gtgaaatcgg catgatcaag gtgctgtcgg aggcctctat cggttccggg gtccggcgcg
     9061 ttgaggcgct ggtcgggctg gatgccttcc gccacctctc gaaggagcac ctgctcgtct
     9121 cgacgctggc cgaccagctg aagtcgcgcc cggaggacct cccggatcgg atcaacgggc
     9181 tggtcgagcg gctgcgcacc gccgagcgcg acctcgagaa ggtccgcgcc gacgccgtgc
     9241 tgtcctcggc cggggcgctt gccgcgggtg ccgaggacat cgacggggtc gcgctcgtgg
     9301 cggtcgccgc accggacggg gtaaccggca acgacctgcg tgccctcgcc tccgacgtcc
     9361 gcggcaggct cgggtcccgg cccggtgtcg tcgcgctctt ctccggcgac ggcgacaagg
     9421 tctccttcgt ggtggccacc acggccgcgg cgagggaccg cggcctggcc gcgggcgggc
     9481 tgatcccggc gttcgccccg gccgtgggcg ggcgcggcgg cggcaagccg gacctggcgc
     9541 agggcggcgg cacgaacccc accggcatcc cggcggccgt cacggcgctc cgctccgcac
     9601 tccgcggatg acggtggcgg tgtgaggggt cgacggctcg gggtcgacgt cggtgcagtg
     9661 cgaataggcg tcgccgtctc cgatccggcc ggtgtactgg caacgccgct ggtcaccgtc
     9721 ccgcgcgatc tcgaggacgg caccgacctg cggacgatcg ccgacctggt cactgagtac
     9781 gacgtcgtcg gcgtcgtggt cgggctgccg cgcacgctgg ccggacggga gggtgctgcc
     9841 gcggagacgg cgcgggcatt cgccgttgcg ctggaggagg ccgtcgacgt gcccgtcgag
     9901 ctctccgacg agcggctcac caccgtggtc gccacccggc agctgcggga gcgcggggtg
     9961 aagggccgca agcagcgtgc tgtagtcgac caggccgccg ctgtagcgat cttgcagggc
    10021 tggctcgacg cagctcgccg tcgctgaccc ggacatgcgc gcccgcgcgc gccctcgtta
    10081 gtgtcggaac cgtgaccccg gacggctggg accagcgagt accgccccac tcgagtggtg
    10141 gggccgacga ctcggtggcg actggaccga tcgacctata cccctcccac caggaggagt
    10201 acacgtcccg gcacgcgcag cagcggaccc gcacacgccg ggggcgaagg cgcagcggcg
    10261 tgctgctgct cgcggtgctg ctgctgctgg ggggcgtcgc cggtggcggc tactacgtgt
    10321 actcgtcggt gttcgccacg cctgacttcg agggcccggg cagtggcgac gtcatcgtgc
    10381 aggtgcatga cggggactcg accacacaga tcggcagcac gttcgcccag cgcggcgtcg
    10441 tggcgggcgt ccaggcgttc accgaggccg cggcggagga gccccggatc cgatcggtgc
    10501 agcctggcta ttaccagatg cggctgcgga tgtcgggcgc ggcggccgtc gcacagctgc
    10561 tggagccgag cgcgcgggtc ggtcagctgg agatccgcgg cggggtccag ctcgacgaca
    10621 ccagagcgtc cgacggcacc gtgacgcccg gcgtgctcag cctgatctcg aaagccacgt
    10681 gcatcgagca gaacggcacg gagcagtgcc tcccggtcga ccagctgcga gccgcgatga
    10741 tggacaccga tccggccgca ctcggcgtgc cggcgtgggc gctggaggag gtcggccggg
    10801 ccgaaccggc gcgccgcctg gaaggcctgc tgatgccggg ccgctacgac gtggcgcccg
    10861 gcactcccgc cgtcgacgtg ctgcgtggtc tgctggccac gtcggacgcg cggctcgagg
    10921 cggccgggtt ggtggccgga gcccagagca tcggctccac cccgtaccac gtcctgacga
    10981 tcgcctcgct ggtggagaag gaggccatta ccccggacat gcccaaggtg gcccgcgtga
    11041 tctacaaccg gctcggggcg ggccagcggc tcgagctcga ctccatggtc aactatccgc
    11101 tcgacctgca ggcgctgcgc accaccgccg atgacagggc gcgcccgggg ccgtacaaca
    11161 gctacgtcgt cgccggcctg ccgcccaccc cgatctcggc gccggggagc gcggcgatcg
    11221 aggcggcact cacgcctgcg cccggtccct ggttcttctt cgtccgctgc cagacggacg
    11281 gcacgtcatg cttcgccacg accttcgccc agcacgccac caacgtccag gaggccaggg
    11341 ctaacggcgc cttctgacct cggcgggcgg acgagaagcc tcagctcgcc gggcggccga
    11401 agaaccccgg cggcggttgg ggcgacggga tcgcggtgcg gtccgacggc ccgcgcgcag
    11461 ctccggcgaa gcgtccgacg tcctcgccgg tgagcaccct ggccgggaac gggtcggccg
    11521 ccgttcgccg caccagcacg tccacgggga gcggtgccgc gctgccgagg agcacgaggt
    11581 tgccgaaccg gcgtccgtgg aggatctccg gcgaggcgag taccgccact tcggcgaacc
    11641 tggccagtgc tgcggccacc tgcgaccgcg cgaagctcag ctccccgccg tcggcgacgt
    11701 tggcggcata caccccggcc ggcgtgagcg tgtccgcagc ggagtcgagg aactccgtcg
    11761 acgtcaggtg cgccggagtg cgggcaccgg cgaacgcgtc gagcacgagc aggtcaaccg
    11821 agcggggtgc gacccgggcg agcgcggccc gcgcgtcagc gacggtgacc tcgatcccca
    11881 gcccgtcggc gggcagccgg gatgcgacga gctccaccag ctcggcgtcg agctcgacga
    11941 cctgctgggt cgatctcggc cgcgtggtgg cgacatatcg ggccagcgtc cacgcgccgc
    12001 cgccgaggtg cagcacccgc agcggcgctg cgggcggtgc catcagatcg acgacgtggc
    12061 cgagccggcg cacgtactcg aactccagat gggtcggatc gtcgaggtcg acgtgcgact
    12121 gggcggtgcc gtcgagcagc agagtccagc cctgcggccg gtccgggtcg cccaagagct
    12181 cggcgtggcc gtggtcgacc tcggcgcgca ccgtggtccg tgccgcccgc tccgctcgtc
    12241 cgcacacgcg tcgatcctgc ccgctgcgtg ccgtcacccg gtggggcggg cccgcccgtt
    12301 gacgtgatcg agccgcagcg tgagcaccag ctgccgtttg tgatcatcgc cgagcgatac
    12361 ccgtcccagt ggggatgggg gcgatcatcc cgcttcaggg tgatcaggat gccgcccgcg
    12421 ctgctgcgcg aacagcgcca ccagcgcggt gtcgatggcc ctactcgtcg acatgcctgc
    12481 tctgtgcctc acgcgcaggg tggtaccacc ctgcgccaac gtcgcgaggc accgcagtca
    12541 tgccgcggtc tgttgtccac ctggtgcgtc gtccatccac agttccgtcg aggccctggt
    12601 cggcgggcgc cggtcggccg aggatcgacg aggtggggga tggcgtgagg gatcgggaca
    12661 gggcccgagg tgccgcggcg gtgggacggc cggtggtgct ggtcgctgta ctgggcgcga
    12721 tgctgacggt gggtctgccg ctggctctgg tgggggccgg cggcccggtg gccgtggttg
    12781 ccgcgggaat ctgtggtggc gtcggtggcg gtgccgcggg cgcggcgggg cgggtgctgc
    12841 tgggacggct gcgccggggg gcacctatcc ggcccccgtg gtgcgagctc gcggtcggtg
    12901 cgctgtgggc cggcacggtc cccgcgtggg cggccggggt tctgccgacg gcgtgggttc
    12961 cggcgctgct cgggctcagc tggctcggcg tggtggccgg agcggtcgac gtgacgcatc
    13021 gcaggctgcc cgacgcgctg acgctgccgg cgctccccct cgcgctggtg ttcctgctgc
    13081 cgctcggcgc cgacgcggtg ctgcgcggtg cggccggcgc cgcggtggcc gcgggcgcgc
    13141 acgccgccgt gcacctgctc gcctgcggcg cgatgggtgc gggtgacgtg aagctggcgg
    13201 ccccgctcgg cgcggtgctt gcggccgcgg ggtggcccgc gctcgtcctg gctgggctgc
    13261 tggcagcggt cgtcaccgcg gcgctcggtg ccactggcct ggccacccgg cgactgaggc
    13321 gcggggcggc gctaccgcac ggtccgtcga tgctggtggc cgcctggctg gtcacctcgt
    13381 ggctggtcgc cacgggtgcc gtgacgggtc acaccggagg ggagtgagag aattgcgctc
    13441 gtgctgcgct ggatcaccgc cggggaatcc cacggtcctg cgctggtcgc cgtgctcgag
    13501 ggcatggtcg ccggcgtcga gatcaccacc aaggagctgg cctccgagct ggcccgccgc
    13561 cgactgggct acggccgggg cgcgcggatg gcgttcgagg ccgacgagct cgaggtgatc
    13621 ggcggggtcc gtcacggggt cacccaaggc gggccggttg cggtgcggat cggcaacacc
    13681 gagtggccga agtgggagac cgtcatggcc gccgaccccg tcgaccccga gctggtcgcg
    13741 gggcgggccc gcaacgcgcc gctgacccgg ccccgcccgg gacacgccga catggccggc
    13801 atgaccaagt acggcttcga ggacgcgcgc cctgtgctcg aacgcgccag cgcacgggag
    13861 actgccgcgc gggtcgtgct gggcaccgtg gccaaggcgt tcctgaagca ggcgttcgac
    13921 gtcgacatcg tgtcccacgt cgtcgcgctc ggctccgtgg aggcgcccga cgacgagcag
    13981 cccggtccag gagatctgga gcgcgtggac gccagcccgg tccgggccta ctccgaggcc
    14041 gccacccacg ccatggtggc tgaggtggat gccgcgcagc gggacggcga caccctcggg
    14101 ggcgtggtcg aggtgatcgc cttcgggctg ccggtcgggc tgggctccta cgtgcagtcc
    14161 gaccgcaggc tcgacgcgcg gctcgccggt gcgctgatgg gcatccaggc gatgaagggt
    14221 gtggagatcg gtgacgggtt ccgcacggcc cggcgccggg gcagcgcggc gcacgacgag
    14281 atgattcccg gcgacccggt gaagcgcctg tccaaccggg ccggggggat cgaaggcggg
    14341 atgtccaacg gcgagcccgt cagggtgcgg gtggcgatga aaccgatctc cacggtgccc
    14401 cgcgcactgc agacggtcga cgtcgctacc ggcgaggccg ctcaggccat ccaccagcgc
    14461 tccgacgtgt gcgcggtgcc tgcggcggga gtggtggccg aggcgatggt cgccttggtc
    14521 ctggccgacg ccgcgctgga gaagttcggc ggcgactcgc tgtccgagac ctgccgcaac
    14581 ttccgggcct accgggacac cggggcgcac tgagcgcggt gctgtggtcg gagccgtgcc
    14641 gacccggggc ctgccggcgt cgagccggcc gtgtcggcgc ggcccggtca ccgacatcag
    14701 ttggttcgcc actccgactg gattttccgg cctgtaaccg ggctccaccc tcggagtgcc
    14761 tctgctccga cgcgccgctc gtgccggaga agcagcccag gcagcggctc aacccccgga
    14821 aggggtccag ggatcatgcc ctgacgggcc gggccaagaa acgctcaccc gggcgaggcg
    14881 cccagagcgc ggctcagtcc ccgaagcggt accggcgcct gcgccgggtg gcagcgagga
    14941 acgcggctgc gaggtccgtc tcagcggagg tgtcgtccgc cggggcgtcg tccgcgttgc
    15001 tgctctcgcc cgcgaacgcg gcgaaaggcg tgccggtgcc tgcgtcgccc gggttgcgca
    15061 gctgcggctc caggtgggcc tgctgccggc gccgggggag ggggagctgc tcgcgtgccg
    15121 gaggtggagg gtcgtccgcc gtgcggagca caggtggact ctcgcggccc tggcgatgcg
    15181 tcgtccgccg gtccgccggt cgcggcgccc cacggccgcc ctgtccgccc ccgccgtcgt
    15241 ccatcgacca ctccgatcgt gcaactccac gcgctgtttc gtcacggccc ggcatcgccg
    15301 cagaggttgc agagtaattg tcgccgtgat cgacagcgta tccggctgtg tatagggcgg
    15361 gcagccggga gcacgatcgc ggggagtgag cggcatctcg ctgtggcgga gggcctccgg
    15421 cgccgccgcc gacggtcact accctggcag tgtgagcctc gaaagacgtc cagcactggt
    15481 catcgtcggt gcgccggggg ccggcaagac cactgtcggt cgggtgctgg cgcgccggct
    15541 cggtgtcgag ttcacggatg tcgacgcgct gatcgtcgag cgggccggga aggctgtcgc
    15601 agacatcttc ctccaggacg gcgaggacgt cttccgagcc atggagcggg aggtcgtcgc
    15661 cgaggcgctg accggcaccg acggtgtgct ggccctcggc ggcggctccg ttcttgcggc
    15721 ggagacgcgg atgcggctgc gcgggcaccg ggtcgtgcac ctggtggtcg ggctcgccga
    15781 cgggctgcgc cgcacgggca tgtccaccgc ccggccgctg ctcgccgggg tcaaccctcg
    15841 tgccacgttc aaggcgctgc tcgacgcgcg cgcgccgctg taccgcgagg tcgcgaccgt
    15901 cgagatcgac acgacgcgac gcagtgccaa ccaggtggcc cggtcggtgc tggaggcggt
    15961 aggggaggtg ctgccgcccg cggcggagga accggtcgac ccggacgacc cgctggaccg
    16021 gcccaccccg cacgacagcg cgctgctgcc ggatcccagc ctgcggccgt gagtgtggtc
    16081 gtcgtggttt gagagacgga ctgtggcctg ttcatcgaca gatcgggtga ctgggctcct
    16141 gtcgccgggc tcacgtcagt gggcaatggt gggggcggca cgagaccacc ggtggcggcc
    16201 gagctcgtcc gtgatgaacc gggcgaggtc ggtgcgggtg agcttccagc cgccctccgg
    16261 ggtggtgccg tcctggacag agcgccaggt ggggatcgtc gtggcggacg ccgggggagg
    16321 agagggcgac gacgcgggcg acgccagtga tctccatcgc ggac
//
LOCUS       DASRSC010000147         1768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_46597,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000147 DASRSC010000000
VERSION     DASRSC010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46597"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..1501
                     /locus_tag="VFE39_07130"
     CDS             158..1501
                     /locus_tag="VFE39_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6258407.1"
                     /translation="MTYRNELVVDDRESGVFRVHRSAMTSPQVLADERAQIFERCWLY
                     VGHDSEIPKPGDYVRRTVCGRPVIFLRDSAGTVRVHFNTCTHRGATLCREESGNTRIF
                     QCFYHAWSFDTTGRLIVTPGADAYAAGFDRSALGLRAPARVESYRGMFFLNHDEHAVS
                     LLDYLGGTVEYLDLVFDQAEEGMRVVGGTHRYSVGANWKLMVENSIDGYHAPTVHATY
                     IDFVKNSGGGQNKAALGTGAGVDLGGGHTVIESTAAWARPIAYWEPFFGEEARPEIEA
                     KRAALTDRYGVERGTRMAETFRNLLIFPNLVINDVAAITVRRIEPVAPDRVALEAWAL
                     APHEEREGSAALQRRLDSFLTFLGPGGFASPDDVEALESCQQGYAAQPDVAWSDISRG
                     MSRIPLPDDELQIRTFWRAWASVLDSAGFPDRVEGVESPGATRTSAPAAAGAVRS"
     gene            1498..>1768
                     /locus_tag="VFE39_07135"
     CDS             1498..>1768
                     /locus_tag="VFE39_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HET6258408.1"
                     /translation="MSTDLATDTALRSRVEDFLYAEAALLDEWRLDDWLQLLSEDARY
                     EVPSTDRPDGRPDTTLMLISDTRPMINARVTRLNSRKAHREFPWSR"
BASE COUNT          268 a          621 c          629 g          250 t
ORIGIN      
        1 ggtccttccg gtcgacggct ctcgaagcgc cgagcgtgac ctgcgtcgtg ttatctgccg
       61 cgttatggga cgggaccggg ctcggataac ggcgtcggta atgacgcggg ccacatgctg
      121 gcgtgcaccc gaatgatcgc caggaggagt caggtcaatg acctaccgca acgaactggt
      181 cgtcgacgac cgggagagcg gcgtcttccg cgtgcaccgg tcggcgatga cctccccgca
      241 ggtgctcgcc gacgagcgcg cccagatctt cgagcgctgc tggctctacg tcgggcacga
      301 ctcggagatc cccaagcccg gggactacgt gcggcgcacg gtctgcgggc ggccggtgat
      361 cttcctgcgg gacagtgcgg ggaccgtccg ggtgcacttc aacacgtgca cccaccgcgg
      421 cgccacgctg tgccgggagg agtccggcaa cacccggatc ttccagtgct tctaccacgc
      481 gtggagcttc gacacgaccg gccggctgat cgtcaccccc ggcgccgacg cctacgccgc
      541 cggcttcgac cgcagtgcgc tcggcctacg cgcgccggcc cgcgtggaga gctaccgggg
      601 gatgttcttc ctcaaccacg acgagcacgc ggtgagcctg ctcgactacc tcggaggcac
      661 cgtggagtac ctcgacctgg tgttcgacca ggccgaggag ggcatgcggg tcgtcggcgg
      721 gacgcaccgc tacagcgtgg gcgcgaactg gaagctgatg gtggagaaca gcatcgacgg
      781 ctaccacgca ccgacggtgc acgccaccta catcgacttc gtgaagaaca gtggcggcgg
      841 gcagaacaag gccgcgctcg gcaccggtgc gggcgtcgac ctcggcggcg ggcacaccgt
      901 catcgagagc accgcggcgt gggcccggcc gatcgcctac tgggagccgt tcttcgggga
      961 ggaggctcgt cccgagatcg aggcgaagcg cgcggcgctg accgatcggt acggtgtcga
     1021 gcgcgggacc cggatggccg agaccttccg caacctgctg atcttcccca acctggtgat
     1081 caacgacgtt gccgcgatca ccgtgcgccg gatcgagccg gtcgccccgg accgggtcgc
     1141 gctggaggcg tgggcgctcg cgccgcacga ggagcgcgag ggcagcgccg ccctgcagcg
     1201 ccggctggac agcttcctca ccttcctcgg ccctggcggg ttcgccagcc ccgacgacgt
     1261 cgaggcgctc gagtcctgcc agcagggcta cgcggcgcag cccgacgtgg cctggagcga
     1321 catctcgcgc gggatgtcgc gcatcccgtt gccggacgac gagctgcaga tccgcacgtt
     1381 ctggcgggcg tgggcgtcgg tgctcgacag cgcgggcttc cccgatcggg tcgagggcgt
     1441 cgagtcgccc ggagcgacgc gcacgtctgc ccccgcggcc gcaggggcgg tgcgttcgtg
     1501 agcacggacc tggccacgga cacggctctg cgcagccggg tggaggactt cctctacgcc
     1561 gaggccgccc tgctcgacga gtggcggctc gacgactggc tccagctgct ctcggaggac
     1621 gcccgctacg aggtgcccag caccgaccgg cccgacgggc gcccggacac cacgctgatg
     1681 ctgatctcgg acacccgccc gatgatcaat gcgcgggtca cccggctcaa cagccgcaag
     1741 gcccaccggg agttcccgtg gtcgcgca
//
LOCUS       DASRSC010000148        18453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1848929,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000148 DASRSC010000000
VERSION     DASRSC010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 18453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18453
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1848929"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1316)
                     /locus_tag="VFE39_07140"
     CDS             complement(171..1316)
                     /locus_tag="VFE39_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HET6258409.1"
                     /translation="MTTPVLPDYGHRSLTEVMPALLAALGVPGQAPELTFEPVRAAAL
                     LLVDGLGSELLRRHAADAPFLASLPDAGPLTVGFPSSTSISLASLGTGLPPGAHGMVG
                     ISFRADDGELLDTLRWTSHGTGRAVDLRDRLPPEDVQAAPTALERAAAAGVAVTVVSK
                     SEFSGSGLTRAALRGGNFRGTHALGDLAAEVIAAVTGPGRRLCYGYHADLDGLGHLRG
                     PGTMAWRLQLSQVDRLAGLIAEHLPPGAVLVITGDHGMVGVDRIFDADTDPVLQQGVQ
                     LLGGDARARHVYARAGAAEDVLATWRSVLRDDAWVVPGEQAIAEGWFGPVAPRVQHRI
                     GDVVVAARGTAAVVRSVAEPVISRLPGQHGSLSTEEQLVPLLVARTP"
     gene            1455..2957
                     /gene="mftF"
                     /locus_tag="VFE39_07145"
     CDS             1455..2957
                     /gene="mftF"
                     /locus_tag="VFE39_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677703.1"
                     /note="Members of this protein family, MftF, are
                     glycosyltransferases, members of PF00535 (glycosyl
                     transferase family 2). The encoding gene is found as part
                     of the mycofactocin cassette, in Mycobacterium
                     tuberculosis, many other Actinobacteria, and occasional
                     members of other lineages. Mycofactocin itself, a putative
                     redox carrier, is a heavily modified derivative of the
                     C-terminal Val-Tyr dipeptide of the mycofactocin precursor
                     MftA (TIGR03969); Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis glycosyltransferase
                     MftF"
                     /protein_id="HET6258410.1"
                     /translation="MTVTGSGIRPGQGALPVGFRVALDRRTRRLDDGAVLLGGAPPRL
                     LRLSAAARQLLADGTCTVTGPTSAALARRLLDAGIGHPVAEPAWAVPSSADVTVVVPV
                     KDRVAGLVRLLAALPSGLGGLVVVDDGSADAAGVRAVAEGAGATVLRHPSARGPAAAR
                     NAGLATASTPLVAFLDSDVVPLAGWLEPLLARFADPAVGLAAPRIVALAPTGTLPRLD
                     GLLAAYEAVRSSLDLGPDPALVVPRSRVAYVPSAALLVRRAAVGAGFDERMQVAEDVD
                     LVLRLHAAGWRLRYEPAARVAHDHRTTLRAWWLRKAYYGTGAAPLALRHPGSVPPMVL
                     SPWSAAVGALLLLGTPRAVQAAVAVSVVAAERLARRLPVDRPRAAAARLVAVGVVGTL
                     AQTADGVTRHYWPVSAAACLVSRRARRTVIFVALAEGIVDWWRHRGRDPYAKPGLFGY
                     VLAHRLDDLAYGAGLWWGALRHRTVQPLRPVGHNSRRRGRSSSEPRQGVR"
     gene            3270..3788
                     /locus_tag="VFE39_07150"
     CDS             3270..3788
                     /locus_tag="VFE39_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET6258411.1"
                     /translation="MAQVRVTVDGVEYADDVEPRTLLVYYLRERLGKTGTLVGCDTSN
                     CGACTVHLNGQSVKSCSVLAVQVDGAEITTIEGIARDGKLHPMQEAFNECHALQCGYC
                     TPGMIMQAIDLLGDNPDPNEQEVREGIEGNLCRCTGYQNIVRAVQSAAAKMKPGSTAD
                     TQSPVSVAGGGE"
     gene            3789..6266
                     /locus_tag="VFE39_07155"
     CDS             3789..6266
                     /locus_tag="VFE39_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET6258412.1"
                     /translation="MTTIAEPTVEFGKSRTRKEDARLITGRSRYTDAITPAGTLHLSV
                     VRSPIAHATITGIDTSPAKQSPGVVGVYTAADLGVEGVGLPCAWPITPDQKAPQRPLL
                     ASSTVHFAGEGVAVVVARSAAEARDAADLVEVDYEPLEPVLDMEAATAEGATLVHPDL
                     GSNVSATWVFDSGEAGTGSNVSEAIAAAEADPDQVVVKRRFRQQRLIPAFMEPRSAVV
                     DPAGEQITFYSSTQIPHILRTMTTVTLGVPESKLRVIAPDVGGGFGGKIGVVPEEMLC
                     VVLAQKLGKPVKWTETRSESLLAAHHGRDQIQDITITAKKDGTVTGLDVKLLADMGAY
                     LGLVGPGVPILGAFMFNSIYKFPAYKFTCTNVFTTKTLTDAYRGAGRPEATFAIERIM
                     DELAVELGRDPLELRGQNWIKHEEFPFTTVCGLTYDSGNYEQATQQAMELFDYAGLRR
                     EQEERRRSGDPVQLGIGISTFTEMCGLAPSRVLGSLSYGAGGWEAASIRMLATGKVEV
                     ITGVSPHGQGHETAFSQIVADQLGVPFEDVEVLHGDTQISPKGLDTYGSRSLVVGGIA
                     IVKAAEKVVAKAKKLAAHLLEASEDDIEFSGGTFTVRGTDKGKAIQEIAFAAFMAHDY
                     PEDMEPSLDSDAVYDPENFSFPHGTHLAAIEIDTDTGHVDLRKYVCVDDIGNVVNPLI
                     AEGQVHGGLAQGIAQALFEEANYDEQGTLVNGTFVDYTLPSAADLPSFDTRVVSTPAT
                     SNPLGVKGVGEAGTIASTPAIINGVLDAIRHLGVTDVEMPTTSQRVWRAIQEAQGRST
                     HETPVDTHSPGAGLGSIDPNNPQGEVQ"
     gene            6263..7114
                     /locus_tag="VFE39_07160"
     CDS             6263..7114
                     /locus_tag="VFE39_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET6258413.1"
                     /translation="MIPSKFDYVKPSSVADAVVALQQGGEDAKILAGGQSLIPVLRLR
                     LATPSVLIDLGGIPELRGIREDGDRIVIGAMTPYHDIIRDDLVKQHVTLLAQATETVA
                     DNQVRHRGTLGGSLAHADPAGDLGAVALALDAELVIAGADGSRTVPASDFFVDYFTTA
                     IGEGEILTEIRFPKYTGWGSHYEKFNRTAQAWSMVAIAAAIRVEGGSIAEARVGLTNM
                     GTTPIRATGVEQALVGQPATADAVRAAADRVTEGTAAPSDADAAADYREHLAKVLTGR
                     AVLAAAG"
     gene            7226..8014
                     /locus_tag="VFE39_07165"
     CDS             7226..8014
                     /locus_tag="VFE39_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6258414.1"
                     /translation="MQLENKFTIEAPIEKAWEALNTPQTVAPCFPGATLQEYEGDSFT
                     GTVKVKLGPISLTYKGKGTYISRDDDNHKVVIEANGRDSRGNGTAKATVTGTLVPDGP
                     SRTAVTMVTDMTITGRPAQFGRGVISDVADKIIGQFSACVASKLAPEQASAAAAPASA
                     GADTAAAAGGSTNGSATTAATATATKGPVLTSAPDAPTAPAAAAPASAAAGVPKAAAP
                     VRSEIDAIDLLDTAGAPVLKRVAPVAGGLLLLLIIFLIKRSRSN"
     gene            complement(8095..8589)
                     /locus_tag="VFE39_07170"
     CDS             complement(8095..8589)
                     /locus_tag="VFE39_07170"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HET6258415.1"
                     /translation="MSTPRIVIATAATVTALLVSGSAIALAGTNGPATVRPAAVGPVS
                     SATTVSTTTPTSSTEPARRTAASGDLSRDDAAAIALAHVGAGRVTKVEQEVEHGRREW
                     KVEVVDGTRRHDVRVDAASGAITRVDANRDDRGNDDRGNDDRGRGSDDSRGHDVGDDK
                     GGDR"
     gene            complement(8775..10067)
                     /locus_tag="VFE39_07175"
     CDS             complement(8775..10067)
                     /locus_tag="VFE39_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6258416.1"
                     /translation="MRRRIMVLVGATTSLVLIAFLVPLALLLRTVADDRAVQAATVEA
                     QSLSSLVGTADRTTLALAVQQLDATSPDAVTVFLADGTVLGTAAERTPLVQLGTLGTS
                     GSGEVEGGREIVFAVRDQAGGAGVIRAFVPDSELGRGVPGAWLLLGALGVGLLALSLA
                     VADRLARSLVRSATDLAAVSHRLARGELAARADPTAPAELGVVAEALNGLATRIAELL
                     REERETVADLAHRVRTPLTALRLDAEALAEQHEAARISTGVDAVQRAVTQAIEQARRR
                     GSGPGAGRTGCDAAAVVRERVAFWAVLAEDTDRETAVDIAPGPLPVALGAADLGACVD
                     ALLGNVFAHTPDGTSFAVRLAPRSEGGALLVVSDAGPGLPDGAAVRRGASGADSTGLG
                     LDIARRSAQQAGGRLDLGRSAHGGLQVVMELGGPQAPA"
     gene            complement(10074..10757)
                     /locus_tag="VFE39_07180"
     CDS             complement(10074..10757)
                     /locus_tag="VFE39_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6258417.1"
                     /translation="MAQVLLIEDDPAIRGALIRGLTERGHAVDSASAALPGLESAVTN
                     RPDLVVLDLGLPDLDGTTVLRMLRGASRVPVIVATARDDEAEIVAVLDAGADDYLVKP
                     FSAAQLDARIRAVLRRSGDTAADPAVVVGGLRVDPRSREASLDGVALDLTPREFSLLH
                     YLAARAGEVVSKRELLTEVWQLPYGGADKTVDVHLSWLRRKLGETAQEPRYLHALRGV
                     GVRISAPQS"
     gene            10870..11349
                     /locus_tag="VFE39_07185"
     CDS             10870..11349
                     /locus_tag="VFE39_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258418.1"
                     /translation="MTTSRRTRTALVALAWAALVVVATLVGMSAVGAIGSGILGDGQQ
                     PLTPAQVDDALAAARVAPGPASGSPAPAPPSPAPSPASSVSATAATDVVASPGGTVVA
                     RCVGDTLEIVSASPAQGFRLHDEREGSRVRFESEESKVEVRVSCQGGRPVGAVEVDN"
     gene            11403..12161
                     /locus_tag="VFE39_07190"
     CDS             11403..12161
                     /locus_tag="VFE39_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6258419.1"
                     /translation="MRGLTDEQIDCFVHDGLVHVEGAFPREVADAGRELLWKATGCDP
                     EDPATWTQPVIRLGDFAQDPFRAAANTPRLHAAFDQLVGAGRWVPRGSLGTFPIRFPH
                     PDAPGDDGWHMEGSFPNAGGGYRLNVRSRGRALLMLFLFSDVGADDAPTRVRTGSHCD
                     VPRLLAPYGDDGAEFFAFCREAVPATEHRPVVTATGAAGDVYLCHPFLVHAAQPHRGT
                     TPRFMAQPPLLPTGELDLESPASPVERAVRDALA"
     gene            complement(12123..12830)
                     /locus_tag="VFE39_07195"
     CDS             complement(12123..12830)
                     /locus_tag="VFE39_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HET6258420.1"
                     /translation="MPGLVPRGRRVLAPGAVHVPDWLDLDRQRELVRACREWASEGPG
                     IRAAVMPNGGQMSVRSVCLGWHWYPYRYSRTRDDQDGSPVTPFPEWLGDLGREALADA
                     YGDPDRARGYRPDIALINHYDAGAKMGLHRDSDEEALDPVISFSIGDPCLFRLGNTES
                     RGRPWTDVELHSGDLIVFGDASRLAYHGVPRLLPAFDLPGTGLAAGRLNITLRVSGLD
                     TQGEASRQGVAHRPFHG"
     gene            complement(13033..13743)
                     /locus_tag="VFE39_07200"
     CDS             complement(13033..13743)
                     /locus_tag="VFE39_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HET6258421.1"
                     /translation="MTAVADPALEHLDWPALQERLDEDGVATTPALLSPEQCASVVAM
                     FDEDERFRSTVVMARHAFGEGSYRYFADPLPPLVQTLRERLYPPLSAIANQWAELLGE
                     RTFPTRLDELLAECAALGQHRPTPLVLRYGPGGYNCLHQDVYGDLTFPLQFLVMLSRP
                     DEDFTGGESVFVEQRPRQQSRPMVMRPRQGEAVIFPVRSRPKRGSRGYHRVQMRHGVS
                     AVHSGDRHVLGIIFHNAR"
     gene            13819..14853
                     /locus_tag="VFE39_07205"
     CDS             13819..14853
                     /locus_tag="VFE39_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HET6258422.1"
                     /translation="MRTGPHNALTDVLGLRVGHASLTGPGALSGTTVVVSPPGGVVAG
                     VDVRGFAPGTRETDLLDPRRMVQRVDAVVFTGGSAFGLSAVTGVMERLEAAGAGFPVP
                     GGVVPIVPAAAVFDLGRGGDLQARPEAATGAAAFDAASEGPVAQGAVGAGTGAVVGGL
                     KGGVGTASAVLDGGAVVAALVVVDSVGSAVDPGSGVLHGARAGLPGEFSDVAVDQAGL
                     AALRAAAAAASPAPAPAPGNATTLAVVATDVTMDKAGCAALAGMGHDGLARALAPVPA
                     ATDGRTVFGLSTAVRPAPEPLELFTVQAVAADVVSRAVAHALLAAATVTTAAGTFRSY
                     AETVSGSVSD"
     gene            14948..15997
                     /locus_tag="VFE39_07210"
     CDS             14948..15997
                     /locus_tag="VFE39_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015102985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET6258423.1"
                     /translation="MKALVYHGPGQKSWDTVADPKPVDPTDIVVQIDTTTICGTDLHI
                     LKGDVPTVEQGRILGHEAVGTVVAVGTAVSGLAVDDRVIVPAITSCGRCSYCKRGMPS
                     HCQATGGIGWIFGHLVDGTQAEYARVPFAETSVHKVPDGVTDEQVLFLTDILPTGYEV
                     GVRNGRVKPGDVVVVVGAGPVGLAAIQTAELAGAATVVAVDVADSRLEHATRFGADHV
                     LNSGGGDVESLIASLTDDGLGADVAIEAVGIPETFDLCTRVVRPGGVVANIGVHGAPT
                     TLHLEDLWIKNVTITTGLVDGTTVPMLMKLVQSGKIAGELFGTHEFALDDMLSAYDTF
                     ADAGANNALKMVIRR"
     gene            complement(15987..16637)
                     /locus_tag="VFE39_07215"
     CDS             complement(15987..16637)
                     /locus_tag="VFE39_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET6258424.1"
                     /translation="MSVERIADAAIAVADADGLAAVSMQRVAGELGFTKMALYRYVPG
                     KTELVALMADVAIGGPPALTGAPHDWRTRLIDWAHHLLAAFLRHPWLLGATVGPRIMG
                     PNELGWMECALAALAATELSGGERLDTVAVVAGHIRSMAQQVGATDRPEAALSSPLVA
                     LMRTHSERYPELTAALASTTTEGGQDDALDFGLHAILDGIGLLVTRRTEGSGAATS"
     gene            16835..18016
                     /locus_tag="VFE39_07220"
     CDS             16835..18016
                     /locus_tag="VFE39_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET6258425.1"
                     /translation="MKSATVLISGASVAGPALAYWLGRRGFAPTVVEIAPRLRDGGYA
                     VDFRGPTNLAVLERMGVLDDLRDVQTGGCAMRFVDETGRQLLHLPPEFTGGDIEVLRS
                     DLSRVLYEHSHDRAEYVFGDSIAGLTETPDGIEVEFEHGAPRTFDLVIGADGMHSGVR
                     RLAFGPESEFVRHLGYYVAGWEVPNHLGLVGESRMYNVPGKVAGVADDHRDPKKAGTT
                     FLFASEELAYDRRDPEQQKKLVADAFVGVGWEAPVLIEELWDAPDLYFDSISRADLPS
                     WSRGRVALVGDAACGATVGGMGTGTAIVGAYVLAGELAAADGDHRGAFARYEDIVRPS
                     AQRCQKGGDRTGKFLAPRTVLGMRARNRLLGTPFLLDLMLKEGRKVSSTVDLPDYRFA
                     A"
     gene            complement(18030..18296)
                     /locus_tag="VFE39_07225"
     CDS             complement(18030..18296)
                     /locus_tag="VFE39_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6258426.1"
                     /translation="MSTTTIRATVRYFAAARAAAGTETEPVELPADGTVEHLLAAIRD
                     RHGAELARVLERCTFLLDEVAVRNRSTPLPDQATLDVLPPFAGG"
     gene            complement(18311..>18453)
                     /locus_tag="VFE39_07230"
     CDS             complement(18311..>18453)
                     /locus_tag="VFE39_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET6258427.1"
                     /translation="AMLRGGSTDAEIADTWRHAMWGKLAGHGIDDPDFLQPDRPMSAI
                     GG"
BASE COUNT         2579 a         6487 c         6831 g         2556 t
ORIGIN      
        1 cgactcgcgg ggcggggacg ggggtcgact cgcgggcggg aaggggcgac tcgcgggcgg
       61 aaagagggcg actcgcggac gggaaggggg cgactcgcgg gcgggaaggg ggcgactcgc
      121 gggcgggaag ggggcgactc gggggcggcg aatgggcgac tcgcgggggg tcagggggtg
      181 cgggctacca ggagggggac gagttgctcc tccgtggaga gggagccgtg ctggcccggt
      241 agccgggaga tcaccggctc ggccaccgac cggacgactg cggcggtgcc ccgggcagcc
      301 acgacgacgt cgccgatgcg gtgctggacc cgcggggcga ccggtccgaa ccagccttcc
      361 gcaatagcct gctcgcccgg caccacccag gcgtcgtcgc gcagcaccga ccgccaggta
      421 gccaggacgt cctccgccgc cccggctcgg gcgtagacgt gccgggcgcg ggcgtcgccg
      481 ccgagcagct ggacgccctg ctgaaggacg ggatcggtgt ctgcgtcgaa gatccggtcg
      541 acgccgacca tgccgtggtc gccggtgatc acgagtacgg cgccgggcgg caggtgctcg
      601 gcgatcagcc cggcaagccg gtcgacctgg ctgagctgca gccgccacgc catggtgcct
      661 ggtccgcgga ggtggcccag cccgtcgagg tcggcgtggt agccgtagca gagccgccgc
      721 cccggcccgg tgaccgcggc gatgacctcg gcggcgaggt cgccgagggc gtgcgtgccc
      781 cggaagttgc cgccgcgcag ggccgctcgg gtgagtccgg agccgctgaa ctcgcttttg
      841 gacacgacgg tgaccgcgac accggcggca gcggcccgtt ccagcgcggt cggggccgcc
      901 tgcacgtcct cgggtggcag ccggtcccgc aggtccactg ccctgccggt gccgtgtgac
      961 gtccagcgca gcgtgtcgag cagttcgccg tcgtcggcgc ggaaggagat cccgaccatc
     1021 ccgtgcgctc ccggcggcag ccccgtgccg agcgacgcga gactgatcga ggtgctggac
     1081 gggaatccga cggtgagcgg tccggcgtcg ggcaggctcg ccaggaaggg cgcgtcggcc
     1141 gcatggcggc gcagcagctc gctgccgaga ccgtcgacga gcagcagtgc cgcagcgcgg
     1201 acgggctcga acgtcagctc gggagcctgg cccgggacgc cgagcgccgc gaggagggcg
     1261 ggcatcacct cggtgaggga gcgatggccg tagtcgggca ggaccggggt ggtcatcggc
     1321 caaggctccc acgcggcaat gtggaccacg caaaatctgc gcagggaagc ccgctatccg
     1381 tcgggccact cacaacgccc gcaggcgcgg agtcctaccc tgcgctggta cgccaggggc
     1441 gtccggaggt ccgcgtgacg gtgacggggt cggggatccg accggggcag ggcgcgctgc
     1501 ccgtggggtt ccgggtcgcg ctggaccggc ggacccgcag gctcgacgac ggcgcggtgc
     1561 tgctcggtgg ggcgccgccg cggctgttgc gactgtctgc ggccgcccgg caactgctcg
     1621 cggacggcac gtgcacggtc actggcccga cgtcggccgc gctggcccgc aggctgctcg
     1681 acgcggggat cgggcacccg gtggccgaac ctgcttgggc cgtgccatcc agtgcggacg
     1741 tgacggtcgt agtgcccgtg aaggaccggg tcgctggtct ggtgcggctc ctcgccgccc
     1801 tgccgtccgg gctgggcggc ctggtggttg tggacgacgg ctcagcggac gccgccgggg
     1861 tgcgggcggt ggccgagggg gcgggcgcca cggtgctgcg gcatccgtcc gcgcgcggcc
     1921 cggccgcggc gcgcaatgcg gggctcgcga cggcgagcac cccgctcgtc gcgttcctcg
     1981 actccgatgt cgtgcccctg gccggctggc tggaaccgct gttggcgcgc ttcgccgatc
     2041 cggccgtcgg ccttgcggcg ccgcggatcg tcgccctggc cccgacggga accctgcctc
     2101 ggctcgacgg actgctggcg gcatacgagg ccgtgcgctc ctcgttggat ctcggcccgg
     2161 acccggcgct ggtggtgccg cgctcccgcg tggcgtacgt gccgagcgcc gccctgctcg
     2221 tgcgccgcgc cgcagtgggc gccggtttcg acgagcgcat gcaggtggcc gaggacgtcg
     2281 acctcgtcct acgcctgcac gcggccggct ggcggctgcg gtacgagcct gccgcccggg
     2341 tcgcccatga tcaccggacg acgctgcgcg catggtggct gcggaaggcc tactacggca
     2401 cgggagcggc ccctctcgcg ttgcggcatc ccggttccgt gccgccgatg gtgctgagcc
     2461 cgtggtcggc cgccgtcggc gcgctgctgc tgctcggcac cccgcgggca gtgcaagcgg
     2521 cggtggcggt cagcgtggtg gctgccgaga ggctggcgcg caggctgccc gtcgatcgcc
     2581 ctcgcgccgc cgcggcccgg ctcgtcgcgg tgggggtggt gggcacgctc gcgcagacgg
     2641 ccgacggcgt gacgcgccac tactggccgg tgtcggcagc ggcgtgcctc gtgtcgcgac
     2701 gggcccgtcg tacggtgatc ttcgtcgcac tggccgaggg catcgtcgac tggtggcgcc
     2761 accgtggccg cgacccgtac gcgaaaccgg gactgttcgg atacgtgctg gcccacaggc
     2821 tcgatgacct ggcgtacggc gccgggctgt ggtggggcgc gctgcggcac cggaccgtcc
     2881 aaccgctccg gccggtcggg cacaacagcc ggcgacgggg gcgctcctcc agcgaaccgc
     2941 gacagggtgt ccgatagcgg gccgtccgtc aggactaatc cgaaagggtg aataagtcct
     3001 gccaataact ccgcttatgc ggatgaaagc gcggatatat cccgtgcctg cggagctgat
     3061 aggaggtgcg cgatctgcac gttcctctgt tgcttgcggc tcggtggcca atgggagggg
     3121 tacggttgca cgggccgatc atcagcggtg tgatgtgtcc gccgccacat cggcgtggcg
     3181 gtccggacag tggttcgcca ctaccaccgg tccctcggaa gtcatgctgg cgcggtgcga
     3241 caccgcggtc gcggttgagg agaagactca tggcacaggt ccgggtcacg gtcgacggcg
     3301 tggagtacgc cgacgacgtg gagccccgta cgttgttggt ttattacctg agggaacggc
     3361 tcggcaagac cgggactctc gtcgggtgtg acaccagcaa ctgcggtgcc tgcaccgttc
     3421 acctgaacgg gcagagcgtg aagtcctgct cggtgctggc cgtgcaggtc gacggcgccg
     3481 agatcaccac gatcgagggc atcgcccgcg acggcaagct gcacccgatg caggaggcat
     3541 tcaacgagtg ccacgccctg cagtgcggct actgcacgcc gggcatgatc atgcaggcca
     3601 tcgacctgct cggggacaac cccgacccga acgagcagga ggtccgggag ggcatcgagg
     3661 gcaacctctg ccgctgcacg ggctaccaga acatcgtccg cgccgtgcag agcgccgcgg
     3721 cgaagatgaa gccggggtcc acggctgaca cccagtcccc ggtgtccgtt gccggagggg
     3781 gcgagtagat gaccacaatc gccgaaccca ccgtcgagtt cgggaagtcc cgcacccgca
     3841 aggaggacgc gcgcctcatc accgggcgca gccgctacac cgatgccatc acgccggcgg
     3901 gcacgctgca cctctcggtg gtgcgctcgc cgatcgcgca cgccacgatc acaggcatcg
     3961 acacgtcccc ggccaagcag tctcccggtg tcgtgggcgt ctatacggcg gccgacctgg
     4021 gcgtcgaggg cgtcggcctg ccctgcgcct ggccgatcac gcccgaccag aaggccccac
     4081 agcgcccgct gctcgccagc agcaccgtgc acttcgccgg cgagggcgtt gccgtcgtcg
     4141 tcgcgcgctc ggccgctgag gcccgcgacg ccgccgacct cgtggaggtc gactacgagc
     4201 cgctcgagcc ggtgctcgac atggaggcgg cgaccgcaga gggcgccacc ctcgtccacc
     4261 ccgatctcgg ctcgaacgtg agcgccacct gggtgttcga ctccggcgag gccggcaccg
     4321 ggagcaacgt ctccgaggcg atcgccgctg ccgaggccga ccccgaccag gtggtcgtca
     4381 agcgccggtt ccgccagcag cggctgatcc cggcgttcat ggagccacgc tccgccgtgg
     4441 tcgaccccgc cggcgagcag atcaccttct actcgtccac gcagatccca cacatcctgc
     4501 ggacgatgac gaccgtgacg ctgggcgtgc cggagagcaa gctgcgggtc atcgccccgg
     4561 acgtgggcgg cggcttcggc ggcaagatcg gcgtggtgcc ggaggagatg ctctgcgtgg
     4621 tgctcgcgca gaagctcggc aagccggtca agtggaccga gacgcgctcg gagtcgctgc
     4681 tcgcggcgca ccacggccgt gaccagatcc aggacatcac gatcacggcg aagaaggacg
     4741 gcaccgtcac cggcctggac gtgaagctcc tcgccgacat gggtgcctac ctcggcctgg
     4801 tcgggccggg ggtgccgatc ctcggcgcgt tcatgttcaa ctcgatctac aagttcccgg
     4861 cctacaagtt cacctgcacc aacgtcttca ccacgaagac gctcactgac gcctaccgcg
     4921 gcgccgggcg gccggaggcc accttcgcga tcgagcggat catggacgag ctcgccgtcg
     4981 agcttggtcg cgacccgctg gagctgcgcg ggcagaactg gatcaagcac gaggagttcc
     5041 cgttcaccac ggtgtgcggg ctcacctacg actcgggcaa ctacgagcag gccacgcagc
     5101 aggcgatgga gctgttcgac tacgcgggcc tgcgccgcga gcaggaggag cggcgccggt
     5161 cgggcgaccc ggtgcagctc ggtatcggca tctcgacgtt caccgagatg tgcggcctcg
     5221 cgccgtcccg ggtgctcggc tcgctgtcct acggggccgg tggctgggag gccgccagca
     5281 tccggatgct cgccaccggc aaggtcgagg tcatcaccgg ggtatcaccg cacggccagg
     5341 gccacgagac ggcgttcagc cagatcgttg ccgaccagct cggcgtgccg ttcgaggatg
     5401 tcgaggttct gcacggcgac acgcagatct cgccgaaggg gctcgacacc tacggatcgc
     5461 ggtcgctggt cgtcggcggc atcgcgatcg tcaaggcggc cgagaaggtg gttgcgaagg
     5521 ccaagaagct cgccgcccac ttgctcgagg cgtccgagga cgacatcgag ttctcgggcg
     5581 gcaccttcac cgtccgcggc accgacaagg gcaaggccat ccaggagatc gcgttcgctg
     5641 cgttcatggc gcacgactac ccggaggaca tggagccctc gctggactcc gacgccgtgt
     5701 acgacccgga gaacttctcc ttcccgcacg gcacgcacct cgccgcgatc gagatcgaca
     5761 ccgacacggg ccacgtcgac ctgcgcaagt acgtctgcgt cgacgacatc ggcaacgtcg
     5821 tgaacccgct catcgccgag ggccaggtgc acgggggtct cgcccagggc atcgcgcagg
     5881 ccctgttcga ggaggccaac tacgacgagc agggcacgct cgtcaacggc acgttcgtcg
     5941 actacaccct gccgtcggcg gccgacctgc cgagcttcga cacccgggtg gtcagcaccc
     6001 cggccacgtc gaacccgctg ggtgtgaagg gcgtcggcga ggccggcacc atcgcctcga
     6061 cccctgcgat catcaacggg gtgctcgacg cgatccggca cctcggcgtc accgacgtcg
     6121 agatgcccac cacgtcgcag cgcgtctggc gggcgatcca ggaggcgcag ggccgttcca
     6181 cccacgagac ccctgtcgac acgcactcac ccggtgccgg cctgggctcg atcgacccga
     6241 acaacccgca gggagaggtc cagtgatccc gtccaagttc gactacgtca agccgtcgtc
     6301 ggtggccgac gccgtcgtgg cgctgcagca gggcggggag gacgcgaaga tcctggcggg
     6361 cggccagagc ctgatcccgg tgctgcgctt gcggctcgcg acgccgtcgg tcctcatcga
     6421 cctcggcggg atcccggagc tgcggggcat ccgtgaggac ggcgatcgca tcgtcatcgg
     6481 cgccatgacg ccctaccacg acatcatccg cgacgacctg gtgaagcagc acgtcacgct
     6541 gctcgcccag gcgacggaga cggtggccga caaccaggtg cgccatcgcg gcaccctcgg
     6601 gggctcgctg gcccacgccg accctgccgg tgacctgggc gccgtggcgc tcgccctgga
     6661 cgccgagctg gtgatcgccg gagctgacgg cagccgcacg gtgccggcga gcgacttctt
     6721 cgtggactac ttcaccacgg cgatcgggga gggcgagatc ctcaccgaga tccggttccc
     6781 gaagtacacg ggctggggga gccactacga gaagttcaac cggacggcgc aggcatggtc
     6841 gatggtcgcc attgcggcgg ccatccgggt cgagggcggt tccatcgcgg aggcccgcgt
     6901 cggtctgacc aacatgggaa ccacgccgat ccgtgcaacg ggcgtcgaac aggcgctggt
     6961 cgggcagccg gccacggcgg acgctgtgcg cgccgccgcc gaccgggtca ccgagggcac
     7021 agcggctccg agtgacgcgg acgccgccgc ggactaccgt gaacatctgg ccaaggtgct
     7081 caccggccgt gcggtgctgg cggccgcggg ctgacgttcg tcgttccacc gtggacgcct
     7141 gctccgatca ccggagtggg cgttcacggc ttttacgcct cggtcggcca gtcggccgaa
     7201 cggggtccga ggaggagaca caccgatgca gttggagaac aagttcacga tcgaggcgcc
     7261 gatcgagaag gcatgggagg cgctgaacac gccccaaacc gtggctccct gctttcccgg
     7321 cgccacgctc caggagtacg agggcgactc gttcaccggc accgtcaagg tgaagctcgg
     7381 gcccatctcg ctcacttaca agggcaaggg cacctacatc agccgcgacg acgacaacca
     7441 caaggtcgtc atcgaggcca acggccgtga ctcgcggggc aacggtacgg ccaaggccac
     7501 cgtgaccgga acgctggtgc ccgacgggcc ctcgcgcacg gccgtcacga tggtcaccga
     7561 catgacgatc accggccgtc cggcgcagtt cggtcgcggg gtcatctcgg atgtcgccga
     7621 caagatcatc ggtcagttct cggcgtgcgt cgccagcaag ctcgcgcccg agcaggcctc
     7681 ggcagcggcc gcacccgcgt ccgcgggtgc cgacaccgcg gccgcagcag ggggcagcac
     7741 caacgggtcc gccacgacgg cggccactgc cacggcgacc aagggcccgg ttctgacgtc
     7801 cgcaccggat gcaccgacgg cacctgccgc cgccgcgccg gcctccgcgg ccgccggcgt
     7861 accgaaggca gcagcgccgg tgcgtagcga gatcgatgcg atcgacctgc tcgacaccgc
     7921 gggcgctccg gtgctcaagc gggtcgcccc ggtggcgggc ggcctcctcc tgctgctgat
     7981 catcttcctg atcaagcgca gccgctccaa ctgaggggcc tcctgccgcg agtcgcagaa
     8041 gagccacgtt catgcaccgg cctgcgtgaa cgtggctctc ctgcacgagg ccagttagcg
     8101 gtcgccgccc ttgtcatcgc cgacgtcgtg gccgcggctg tcgtcagagc cccgccctcg
     8161 atcgtcgttg ccccggtcgt cgttgccccg gtcgtcgcgg ttggcgtcga cccgggtgat
     8221 cgcccctgac gcggcgtcca cgcggacgtc gtgccgacgc gtcccgtcca cgacctcgac
     8281 cttccattcc cgtcggccgt gctcgacctc ctgctcgacc ttggtgaccc tcccggcgcc
     8341 tacgtgcgcc agcgcgatcg ctgcggcgtc gtcgcgggag aggtcgcccg atgcggcagt
     8401 ccgccgggcc ggctcggtgg agctcgtcgg ggtcgtggtc gataccgttg tggcactgct
     8461 cacgggcccg acggcggcgg gtcgtacggt cgccgggccg ttggtgccgg caagggcgat
     8521 cgcggaaccg ctgaccagca gtgcggtgac ggtggctgcc gtggcgatca caatgcgggg
     8581 cgtggacatg tctgctcctc cgggttgtcg tgctgtcgac ccagacggtg ccgatcatcg
     8641 ggctagcgcc gcgctgccgc agtcctaagg ccgcgttagg ccaggccgcg gatggcacgg
     8701 agcacgggtc gggcgagcgg cgcgttcggc cggacctctc gggcgagagc gcgttcgtcc
     8761 gggcccgcag aacctcaggc cggggcctgc ggcccgccga gttccatgac gacctgcagc
     8821 ccaccgtggg cggagcgccc cagatcgagc cggccgccag cttgctgcgc gctgcgacgg
     8881 gcgatgtcga gcccgagacc cgtcgagtcg gcgccgctgg cgccccgccg gaccgcagct
     8941 ccgtcgggaa gtcccggccc ggcgtcggag accacgagga gggcaccgcc ctcggaacgg
     9001 ggcgcgagcc gcactgcgaa agacgtgccg tccggcgtgt gggcgaagac gttgcccagc
     9061 agcgcgtcca cgcaggcgcc gaggtctgcg gcgcccagcg ccaccggcag ggggcccgga
     9121 gcgatgtcga ccgcggtctc ccggtcggtg tcctcggcca gcaccgccca gaacgccacg
     9181 cgctcgcgca ccactgccgc ggcgtcgcag ccggttcgcc ccgcgcccgg cccgctgccg
     9241 cgccgcctgg cctgctcgat cgcctgggtg accgcgcgct gcaccgcgtc gaccccggtg
     9301 ctgatccggg ccgcctcgtg ctgttcggcg agggcctcgg catccaggcg caacgcggtg
     9361 agtggggtgc ggacgcggtg ggcgaggtcg gccacggtct cgcgctcctc gcggagcagc
     9421 tcagcgatgc gcgtggccag gccgttcagc gcctcggcga caacgcccag ctcggcgggg
     9481 gcagtcgggt cggctcgggc ggccagctcg ccgcgggcga ggcggtgcga caccgccgcg
     9541 aggtcggtgg cggaccggac cagtgaacgg gccagccggt cggcgaccgc gaggctgagg
     9601 gcgagcaggc cgacgccgag cgctccgagc agcagccacg cacccggcac gccccggccc
     9661 agctcggagt cgggcacgaa cgcgcggatc actccggcgc cgcccgcttg gtcccgcacc
     9721 gcgaagacga tctcccggcc gccctccacc tccccggacc cgctggtgcc gagcgtcccc
     9781 agctggacga gcggggtccg ctcggccgcc gttccgagga cggtgccgtc ggccaggaac
     9841 acggtgacgg cgtcgggcga cgtggcgtcg agctgttgga cggcgagggc gagggtggtc
     9901 cggtccgcgg tgcccacgag cgagctgagc gactgggctt cgacggtggc ggcctgcacg
     9961 gcccggtcgt cggcgacggt gcgcagcagc agagcgagcg gcacgaggaa ggcgatgagg
    10021 accagcgagg tcgtcgcccc gaccaggacc atgatgcggc ggcgcacgtc gcctcagctc
    10081 tgcggtgcgg aaatccgcac accgacgccg cgcagggcgt gcagatagcg cggctcctgg
    10141 gccgtctccc cgagttttcg gcgcagccag gagaggtgga cgtcgacggt cttgtccgcg
    10201 ccgccgtagg gcagctgcca gacctcggtg agcagctcgc gcttgctgac gacctcgccg
    10261 gctcgggcgg cgaggtagtg caggagcgag aactcgcgcg gcgtgaggtc gagtgcgaca
    10321 ccgtcgaggc tcgcctcccg ggaccgtggg tccacccgca gcccgcccac cacgacggcc
    10381 gggtcggccg cggtatcccc gctgcggcgc agcacggcgc ggatacgggc gtcgagctgc
    10441 gcggcgctga acggtttgac caggtagtcg tcggcgccgg cgtcgaggac ggccacgatc
    10501 tcggcttcgt cgtcgcgggc cgtcgccacg atcaccggca cgcggctcgc cccgcgcagc
    10561 atccggagga ccgtcgtgcc gtcgaggtcg ggcaacccga ggtcgagcac gacgaggtcg
    10621 ggccggttcg tcacggccga ctcgagcccg ggcagcgccg cggaggcgga gtcgacggcg
    10681 tgcccgcgct ccgtcagccc gcggatcaac gcgccgcgga tcgccggatc gtcctcgatg
    10741 agaagcacct gggccacgtg cggaaccgta ggacaatcga gatcgttcca ggcgaatccg
    10801 gcgctgcggc ggcgcgccga taatgcggcc ttaggagcgc cttaaccccc gccacggcag
    10861 gctgcgcgta tgacgacgtc ccggcgtacg cgcacggccc tcgtcgccct cgcctgggcg
    10921 gcgctcgtcg tcgtcgcgac gctggtgggg atgagcgcgg tcggcgcgat cggcagcgga
    10981 atcctcgggg acgggcagca gccgctcacg cccgcccagg tcgacgacgc gctcgcggcc
    11041 gcccgcgtgg caccgggtcc ggcgtccggc tcgcccgcac cggcgccccc gtcgcccgca
    11101 ccatcgcccg cgtcgtccgt gtcggcgacc gccgccaccg acgtcgtcgc gagcccgggc
    11161 ggcacggtcg tggcccggtg tgtgggggac accctcgaga ttgtctcggc cagtccggcg
    11221 cagggcttcc ggctccacga cgagcgcgag ggctctcggg tgcggttcga gtccgaggag
    11281 tcgaaggtgg aggtgcgggt gagctgccag ggcggccgcc cggtcggtgc ggtggaggtc
    11341 gacaactgac cgtcgttcaa atcgggtggc cgtcggagaa ccggtcctgc acagtgacgg
    11401 ccgtgcgagg actgaccgac gagcagatcg attgcttcgt gcacgacggc ctcgtgcacg
    11461 tcgagggcgc gttcccgcgc gaggtggccg atgcgggccg ggagttgctg tggaaggcca
    11521 cgggctgcga cccggaggac cccgccacgt ggacacagcc cgtgatccgg ttgggcgact
    11581 tcgcgcagga tccgttccgc gccgcggcga acacgccgcg gttgcacgcc gccttcgacc
    11641 agctcgtggg ggccgggcgc tgggttcccc ggggaagcct cggcaccttc cccatccgct
    11701 tcccgcaccc ggacgcgccc ggcgacgacg ggtggcacat ggaggggagc ttcccgaacg
    11761 cgggcggcgg ctaccggctg aacgtccggt cgagaggccg ggcgttgctg atgttgttcc
    11821 tgttctccga cgtcggcgcc gacgacgcgc ccacccgcgt ccgcaccggc tcgcactgcg
    11881 atgtcccccg gctgctcgcg ccctacggcg acgacggtgc cgagttcttc gccttctgcc
    11941 gcgaggcggt gcccgccacc gagcaccggc cggtcgtcac ggccaccggg gcggcgggcg
    12001 acgtctacct gtgccacccg ttcctcgtgc acgccgcgca accccatcgc ggcacgacgc
    12061 cgcggttcat ggcccagcca ccgctcctgc ccacgggcga gctcgatctc gagagtcctg
    12121 cgtcacccgt ggaacgggcg gtgcgcgacg ccctggcgtg acgcttcgcc ctgtgtgtcc
    12181 agacccgaca cgcgcagcgt gatgttgagc cggcccgcgg cgagtccggt gccgggcagg
    12241 tcgaacgcgg gcagcagcct cggtaccccg tggtaggcga gtctgctcgc gtcgccgaag
    12301 acgatcaggt ccccggagtg cagctcgacg tcggtccacg gccgtccgcg gctctcggtg
    12361 tttcccaacc ggaacaggca cgggtcgccg atgctgaacg agatgacggg gtcgagcgcc
    12421 tcctcgtcgc tgtcgcggtg cagccccatc ttcgcgcctg cgtcgtagtg gttgatcagc
    12481 gcgatgtccg gccggtagcc ccgggctcga tccgggtcgc cgtacgcgtc cgcgagcgct
    12541 tcgcgaccga ggtcgccgag ccactccggg aacggggtga ccggagagcc gtcctggtcg
    12601 tcacgggtgc gggagtagcg gtaggggtac cagtgccagc ccaggcagac gctgcgcacc
    12661 gacatctgcc cgccgttcgg catgacggcg gcgcggatgc ccggtccttc gctcgcccac
    12721 tcccggcagg cgcgcacgag ctcgcgctgc cggtcgagat cgagccagtc cggcacgtgc
    12781 acggcgcccg gtgcgagcac ccggcgcccc cgagggacca ggccgggtat cacgagcccc
    12841 tcggtggatc cgtcgtcacg ctgcccagtg tcccgcgtat cgcattccgc gtcagtggcc
    12901 tcgactacgc cgagccgaac gcgatgctca cctgacccga gccgcgcgcc cggtccggtc
    12961 ctcacagggg ccgcgggctc tgcgaggacc agggggcggc gagcggaacg tggtccccgt
    13021 gccgcggcgc ggtcaccgcg cgttgtggaa gatgatgccg agcacatgcc ggtccccgct
    13081 gtggacggca ctcacgccgt ggcgcatctg cacgcggtgg tagccgcggg agccccgctt
    13141 cggccggctg cgcaccggga agatcacggc ttcgccctgc cgggggcgca tgaccatcgg
    13201 ccgggactgc tgacggggtc gttgctcgac gaacacgctc tcaccgccgg tgaagtcctc
    13261 gtccggccga ctgagcatca ccaggaactg cagcgggaac gtcaggtcgc cgtagacgtc
    13321 ctgatggagg cagttgtagc cgccagggcc gtagcgcagc accaacggag tgggccggtg
    13381 ctgacccagc gccgcgcact ccgccagcag ctcgtcgagc cgggtgggga acgtgcgctc
    13441 gccgaggagc tcggcccact ggttggcgat cgccgacagc ggggggtaga gccgctcacg
    13501 caacgtctgg accagcggcg ggagcgggtc ggcgaagtac cggtagctgc cctcgccgaa
    13561 ggcgtggcgg gccatcacga cggtgctgcg gaaccgctcg tcctcatcga acatcgcgac
    13621 cacgctcgcg cactgctccg gcgacagcag cgccggggtg gtggcgacgc cgtcctcgtc
    13681 gaggcgctcc tgaagtgccg gccagtcgag gtgctccagt gcgggatccg ctacggcggt
    13741 cattgctcca ctgtcgtcga tccccggccc ccgtgctggc gggaaacggt caccggcctg
    13801 agggcaggat gaccgctcat gcgcaccgga ccgcacaacg ccctgaccga cgtgctcggc
    13861 ctgcgcgtcg ggcacgcctc gctgactggt ccgggggccc tgagcggcac gacggtggtg
    13921 gtttccccac cgggaggcgt cgtcgcgggc gtcgacgtcc gcggcttcgc gcccggcacg
    13981 cgcgagacgg acctgctcga cccgcgcagg atggtgcagc gtgtggacgc cgtcgtgttc
    14041 accggcggca gcgccttcgg gctgtccgcg gtgacgggcg tgatggagcg gttggaggcg
    14101 gcgggtgccg ggttccccgt gcccggcggc gtggtcccga tcgtgccggc tgccgccgtg
    14161 ttcgacctcg ggcgtggtgg cgacctccag gcccgaccgg aagcggccac cggggccgcc
    14221 gcgttcgacg ccgcgagcga ggggccagtc gcccagggag ccgtcggagc gggtacggga
    14281 gcggtcgtcg gtgggctgaa gggcggtgtg gggacggcga gcgcggtgct cgacggagga
    14341 gccgtggtgg cagcgctggt cgtcgtcgac tccgtggggt cggccgtcga cccggggtcc
    14401 ggggtgctcc acggggcgcg cgccggactg cccggggagt tctcggacgt tgccgtcgac
    14461 caggccgggc tcgccgcgct ccgggccgcg gccgcggccg cgtccccggc accggcaccg
    14521 gcaccgggaa atgcgacgac gcttgcggtg gtggccaccg acgtcaccat ggacaaggcg
    14581 gggtgcgccg ccttggccgg gatgggccac gacggcctgg cccgcgccct cgcgcccgtg
    14641 ccggccgcca ccgacggccg cacggtcttc gggctctcga ccgccgtccg gccggccccc
    14701 gagccgctcg agctcttcac ggtgcaggcc gtggcggccg atgtggtgtc gcgtgccgtc
    14761 gcgcatgcgc tgcttgctgc ggccaccgtg acgacggccg cgggcacctt tcgcagctac
    14821 gcggagacgg tgagcgggag cgtgtcagat tgagcgtgtc agattgagac agcgagccgg
    14881 cgggatcggc ctagcgtgcg tgggcgccgt cacagcagtg acggcgcacc gttgcgagga
    14941 gaagccgatg aaggcgctcg tctaccacgg tccgggccag aagtcctggg acaccgtcgc
    15001 cgatccgaag ccggtggacc cgaccgacat cgtcgtgcag atcgacacca cgacgatctg
    15061 cgggacggac ctgcacatcc tgaagggtga cgtgcccacc gtcgagcagg gccgcatcct
    15121 cgggcacgag gcagtgggca ccgtcgtcgc ggtcggaacg gccgtgagcg gcctcgccgt
    15181 cgacgaccgc gtgatcgtcc ccgccatcac gtcgtgcggg cgctgctcct actgcaagcg
    15241 cggcatgccg agccactgcc aggccaccgg tggcatcggc tggatcttcg gacacctcgt
    15301 cgacggcacg caggccgagt acgcgcgggt cccgttcgcc gagacgagcg tgcacaaggt
    15361 gcccgatggg gtgaccgacg aacaggtgct cttcctcacc gacatcctgc ccacggggta
    15421 cgaggtgggc gtccgcaacg gccgggtgaa gccgggtgac gtggtcgtgg tcgtcggggc
    15481 cggcccggtc ggcctggccg cgatccagac cgcggagctc gccggcgccg ccacggtggt
    15541 cgcggtggac gtcgccgact cgcggctgga gcacgccacc cggttcggcg cggaccacgt
    15601 gctcaactcc ggcggtggcg acgtcgagtc gctcatcgca tccctcaccg acgacggcct
    15661 cggagccgac gtcgccatcg aggccgtcgg gatcccggag acgttcgacc tctgcacccg
    15721 ggtggttcgg cccggcggtg tcgtcgcgaa catcggggtg cacggcgccc ccacgacgct
    15781 gcacctcgaa gacctgtgga tcaagaacgt gacgatcacg acgggcctcg tcgacggcac
    15841 caccgtgccg atgctgatga agctcgtgca gtcggggaag atcgcggggg agctgttcgg
    15901 cacccacgag ttcgcgctgg acgacatgct gtcggcctac gacaccttcg ccgacgccgg
    15961 cgcgaacaac gccctgaaga tggtgatcag gaggtagccg cacccgatcc ctccgtgcga
    16021 cgggtcacga gcaggccgat cccgtcgagg atggcgtgga gcccgaagtc gagggcgtcg
    16081 tcctggccgc cctccgtagt cgtcgacgcg agggcggcgg tgagctccgg atagcgctcg
    16141 ctgtgcgtgc gcatcagcgc cacgaggggg ctgctgagcg ccgcctcggg gcgatcggtt
    16201 gcccccacct gctgagccat ggagcggatg tggcccgcca ccaccgcgac cgtgtcgagg
    16261 cgctcgccgc cgctgagctc ggtcgcggcg agtgcggcga gggcgcactc catccacccg
    16321 agctcgttcg gccccatgat ccgtggacca accgtggcgc cgaggagcca cgggtggcgc
    16381 aggaacgccg cgagcaggtg atgtgcccag tcgatgaggc gggtccgcca gtcgtgcggt
    16441 gcaccggtga gcgcgggcgg cccgccgatc gccacgtccg ccatgagcgc gaccagctcc
    16501 gtcttccccg ggacatagcg gtaaagcgcc atcttggtga agcccagctc gccggccacc
    16561 cgctgcatcg agaccgccgc caggccgtcg gcgtcagcca ccgcgatggc cgcgtcggcg
    16621 atgcgctcga cgctcatggc cggcttcggt ccacgacctg ggcgccgggg tgaaccccag
    16681 agcagctcca ctgcggctgc ctggtcgccg aacggtccct cgtcgtccgt gcgcgtcact
    16741 cgtccccttc cgggctggtg ttcgcgtcct aactgtgtct accatacaca aaacagtgtc
    16801 cggtggacgc agttctgacg agaggacctg gatcatgaag agcgcgaccg tgctcatctc
    16861 cggtgcgagc gtcgccggcc ccgcgctggc gtactggctg ggccgccgcg gattcgctcc
    16921 gaccgtcgtc gagattgcgc ccaggctgcg cgacggcggc tacgccgtcg acttccgcgg
    16981 gccgacgaat ctggcggtgc tcgagcgcat gggagtgctg gacgacctgc gcgacgtcca
    17041 gaccggcggg tgcgccatgc gcttcgtcga cgagacgggc cggcagttgc tgcacctgcc
    17101 cccggagttc acgggaggag acatcgaggt gctgcgctcc gacctgtccc gcgtcctgta
    17161 cgagcacagc cacgaccgcg ccgagtacgt cttcggcgac tccatcgcag ggctgaccga
    17221 gacgcccgac ggcatcgagg tcgagttcga gcacggcgcg ccgcggacgt tcgatctggt
    17281 gatcggcgcc gacggcatgc actccggggt gcgccgactg gccttcgggc cggagtcgga
    17341 gttcgtccgg cacctcggct actacgtcgc gggctgggaa gtccccaacc acctggggct
    17401 cgtcggcgag tcacggatgt acaacgtccc gggaaaagtc gcgggcgtcg ccgacgacca
    17461 ccgggatccg aagaaggccg gtacgacgtt cctcttcgcg tccgaggagc tggcctacga
    17521 ccgacgagat cccgagcagc agaagaagct ggtcgccgac gccttcgtgg gcgtgggctg
    17581 ggaggctccg gttctgatcg aggagctgtg ggacgcgccg gacctgtact tcgactcgat
    17641 cagtcgcgcc gacctgccgt catggtcgcg gggccgggtg gcgctcgtcg gtgacgcggc
    17701 gtgtggcgcc acggtcggcg ggatgggtac cggcacggca atcgtcgggg cgtacgtact
    17761 ggcgggcgag ctcgcggccg cggacggcga ccaccgcggg gcgttcgccc ggtacgagga
    17821 catcgtgcgc ccctccgccc agcgctgcca gaagggcggc gaccgcaccg ggaagttcct
    17881 tgcgccgcgc acggtactgg ggatgcgggc gcggaaccgg ctgctcggta ctcccttcct
    17941 gctcgacctc atgctcaagg aggggcggaa ggtcagcagc acggtggacc tgccggacta
    18001 cagattcgcg gcgtgacggg agccggagct cagccgccgg cgaacggtgg gaggacgtcg
    18061 agcgtggcct ggtcgggtag gggggtgctg cggttgcgga cggcgacctc gtcgagcagg
    18121 aaggtgcagc gctccaacac gcgggccagc tcggcgccgt gccggtcgcg aatggcggcg
    18181 agaaggtgct cgacggtgcc gtctgccggg agctccaccg gctccgtctc cgtgccggcg
    18241 gctgctcggg ccgcggcgaa gtagcgcacg gtggcgcgga tggtggtggt gctcatcccc
    18301 gacatctttc tcatccgccg atagcgctca tcgggcggtc cggctgcagg aagtcagggt
    18361 cgtcgatgcc gtggccggcg agcttgcccc acatggcgtg gcgccaggtg tcggcgatct
    18421 cggcatcggt cgaaccgccg cgcagcatcg ccc
//
LOCUS       DASRSC010000149         1541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4472381,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000149 DASRSC010000000
VERSION     DASRSC010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4472381"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..501)
                     /locus_tag="VFE39_07235"
     CDS             complement(<1..501)
                     /locus_tag="VFE39_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET6258428.1"
                     /translation="MEQLRFEGMPRRLYSCTPTRLNTWLGCPRKYRMSYLDRPGPPKG
                     APWAHNSLGASVHNALAAWWRLPLARRTVEAAGSLLERGWIAEGFAGDAQSAQYRAWA
                     RGLVEAYVAGLDPSDEPVGVERTVATRTDTIAVSGRIDRLDSRVSASGAELVVVDYKT
                     GRHVLTM"
     gene            complement(597..1244)
                     /locus_tag="VFE39_07240"
     CDS             complement(597..1244)
                     /locus_tag="VFE39_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6258429.1"
                     /translation="MVAARIERVWTPRVAEHDADADAAETNAWVIGDDDEVIVIDPGD
                     DVGGILDAIGDREILAVICTHGHAAHTAAAVEVAARDEAPVALHRADKLAWRTAHRED
                     DPDIEMADAGVFEVADVQLEVLHTPGHTQGSVSLYCPELGAVFTGDALLASGPAPHDG
                     FFPDFPRQLTAIGEKLLDLPPETRVLSGHGEESSIGDAVKRFDEWVTAGPSAGGS"
     gene            complement(1316..>1541)
                     /locus_tag="VFE39_07245"
     CDS             complement(1316..>1541)
                     /locus_tag="VFE39_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HET6258430.1"
                     /translation="IAGLAAAGLAGVEVFHPDHDDSERISLTALARTLSLITTGGSDD
                     HGSFNSSGIGTETTPEDQYGLLIAEARPRA"
BASE COUNT          211 a          571 c          521 g          238 t
ORIGIN      
        1 catggtgagc acatgccggc cggtcttgta gtccaccacg accagttccg ccccggaggc
       61 ggacacccgg gagtccagcc ggtcgatccg cccggacacg gcgatcgtgt cggtacgggt
      121 cgccacggtc cgctccacgc cgaccggctc gtcggacggg tcgagccccg ccacgtaggc
      181 ctcgaccagt ccgcgtgccc aggcccggta ctgcgcggac tgcgcgtcgc cggcgaaccc
      241 ctcggcgatc cagccgcgct cgagcagcga cccggccgcc tccaccgtgc gccgcgcgag
      301 cggcagccgc caccaggccg cgagcgcgtt gtgcacgctc gcgccgaggc tgttgtgcgc
      361 ccacggcgcc cccttcggcg gccccggccg gtccaggtag ctcatgcggt acttgcgcgg
      421 gcaccccagc caggtattga gtcgcgtggg cgtgcaggag tacagccgcc tcggcatccc
      481 ttcgaaccgg agctgctcca ccgcgtacac ctgcctttaa aactcgaaac ccttgtcggg
      541 agtccggggg tcgtcccccg ggctagcact gtcccgaacg gatcgccgcg cctgtctcag
      601 ctcccgccgg ctgacggccc agcagtgacc cactcgtcga accgcttcac ggcgtccccg
      661 atcgaggatt cctcgccgtg ccctgagagc acccgggtct ccggcggcag gtcgagcagc
      721 ttctcgccga tcgccgtgag ctgccggggg aagtcgggga agaagccgtc gtgcggtgcc
      781 gggccggagg cgagcagcgc gtcgccggta aacacggcgc ccagctcagg gcagtagagg
      841 ctgaccgaac cttgcgtgtg gcccggggtg tggagcacct cgagctggac gtcggcaacc
      901 tcgaagacgc ccgcatccgc catctcgatg tccgggtcat cctcccggtg cgcggtgcgc
      961 caggccagct tgtccgcgcg gtgcagcgcc accggcgcct cgtcccgcgc ggcgacttcg
     1021 acggcggcgg ccgtgtgggc ggcgtgcccg tgggtgcaga tcacggcgag gatctcccgg
     1081 tccccgatcg cgtccaggat ccctccgacg tcgtcacccg ggtcgatgac gatcacctca
     1141 tcatcgtcgc cgatcaccca cgcgttagtc tcggccgcgt cagcgtcagc gtcgtgttcc
     1201 gcgacgcgcg gcgtccatac ccgttcgatg cgagctgcca ccatcggctc ccttatgcga
     1261 ccctaagctc tggctaagcg tagctctccg tgcacgtgca tgcgtccgta tccgttcagg
     1321 cccgggggcg cgcctccgcg atgagcagcc cgtactggtc ttccggcgtc gtctcggtgc
     1381 cgatgccgga agagttgaag gagccgtggt cgtcgctgcc gcccgtcgtg atgagcgaga
     1441 gggtacgcgc gagggcagtc aggctgatcc gttcggaatc atcgtggtcg ggatggaaca
     1501 cctccacgcc cgccaggccc gcggcggcca gcccggcgat c
//
LOCUS       DASRSC010000150        34036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1849496,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000150 DASRSC010000000
VERSION     DASRSC010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 34036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34036
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849496"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..555
                     /locus_tag="VFE39_07250"
     CDS             <1..555
                     /locus_tag="VFE39_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET6258431.1"
                     /translation="LVTCNSYRNPDLLADMARTVDHISGGRLVLGIGAGWAQRDYDAY
                     GYPFGTAGSRLADLADALPRIKKRWAQLNPPPTREIPILIGGGGEKKTLRYTAEHATI
                     WHGIGAPATIAHKVQVLDAHCADIGRDPAEIERSAKVDVPPEEVADELLAAGITLFTV
                     STSGPDYDLGLLKSWLAWRDSREA"
     gene            complement(1051..6081)
                     /locus_tag="VFE39_07255"
     CDS             complement(1051..6081)
                     /locus_tag="VFE39_07255"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HET6258432.1"
                     /translation="MPDMLTPDPLACALPDLVLEAIAGQVGDALRDHGAGHCVRIDSI
                     RRGDAAQLVETLRDRLPSGVADVHVLVDQPGEADGKLSIPAERAVELRNRKERQLVLM
                     VPVGSGSAASSLDNSFARIDVTLLLGAAAELLVATIADTDLQAGVRQVARELGRTRPV
                     EAWARYVAAVVGGPSWDTAGAALWMVGLVPDLGGPDLLGRLSRNAACARAISRPSRAV
                     ASVTDRLTVAELQEGHARDRIARYLSRPDVDLSDPPAWAAPLAQPPHELTFEKWPLVE
                     RRPVEVGSVRLDPFLKEDGTLRTGTRLSQESPGDLPFVETGPDTPGTVTVVWRTDPPK
                     TDAIDRWLLEALPPVDLRDPDTEPVARQTVKGDKRRATVRIDFAEEDLTEGALLVVRL
                     TAIDADGQPVLLRSGAEAVDESQQFGVRWEEDPVGGPTRRASTPSLAQARLDAALEGQ
                     DDLREDAPSWNGGAFSVRLGGRRTALLALSPALTSLQDKLLQGRGRAIAWEADGRLGE
                     VLDPEALEPLAGNLPPAFAERRRRLFDRLDARRPRFAVETLAWDDELRQEVQAYCQAY
                     RRALDSAADDESRGALLALDTVRLSVDTVGHDPIAAMIALPLQPLRLAWAAEYDATLT
                     RWAGELASLGRSTAKRRQSVDARLTRRVTPANLPFAMYGVDGSPFVYVREATLGTGVY
                     LHTAEAEPGAAVQAVFEVLGLDRRDVPPDLPPSTVAERISAYRSVNPGQDALRILAYN
                     VGSGELLARALDESVLREPDTDEELAAAPARLELTAYSRRPSFTDPVPALTDLQRAVA
                     TQQVRGARSHLTPPLGLTVRSHDRLAADLDAAHIGVIADLAITRAEDAAGVAAVAVPD
                     AATSFRNLLTPAQTQRVTAPTPVWRTAPAVRARNRDGAADAVEAHRSYQAALAIALGY
                     PGGPLALTARLGPGELASLRAAHERADWVVTLDRNLGIDLFATASGSDAPPYVLDYAP
                     DFLEGLGPRLTVTTTHRGDVQRLLADAMTGLDLAAVDESVRVVLDHLQVVSGRLALRL
                     VGRSSLATEAVSLAALVAHLRGRGELDGCLVVPVDAHQEVFGDTRGDAGARRCDVILV
                     RTTARTLRMECVEVKSRRAAALPAALVDDIVDQLDATVTMLHDTFFRTDPPRIDAELQ
                     RARLGGILRHHADRALAMGLLDDRRRGEVERMVEKVEDGALVPEISRRGYVVSLGGKA
                     GFPAEHRGVKIDVLTAADLGPAGFVSRRASGSPQPEWAPESPPTSLPTAEPERVSPIL
                     PELVPQRPSPRPRPSPRPRVAVEPAATAGSVQSTTELPSVTPRRAEVQQSAPLQPLHE
                     APPPDPVLAQPVPAALLPQVVEVTLGQDAHAEDVPWRISTSGSPHLFVLGIPGQGKSV
                     TTRRILNSFAEQGLPALVIDFHGDMAAAPAGGAAVLDASMGLPISPFEIDEPARYREA
                     SWELAEVIGYVCGLGEIQRNVVYEGVRGIYEQHGFPRPSHPDDWPTMDELAEAVTSAE
                     ATSRSRNVAARLRPLSDFGLFVDPEHGRGFADLLRQGVVLDVHGLMEQVQRAAGAFVL
                     RKVYREMFRWGQTKQLRLAVVLDEAHRLARDVTLPKIMKEGRKYGVAVVVASQGVDDF
                     HKDVLGNAGTKVAFRCNYPQSKTVAGFLRGRSGQDVAEALEQLSVGQAYVSTPEHAAA
                     RKVFMARD"
     gene            complement(6081..7733)
                     /locus_tag="VFE39_07260"
     CDS             complement(6081..7733)
                     /locus_tag="VFE39_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258433.1"
                     /translation="MTISLPTPLRTYQYRPLTVFSLLDTDTDRVLPQLFEMCVKGGRS
                     SRSRTDARDYDGYRRALLASGTVEGFDDPRDQALIDGWLRSCVVDMGAVGRAKTGEQM
                     LAVAPLTLAAYRAGLPKERQRHRGIDDTVYRLLLEAIDREAPGGGSGGTAAALRELVT
                     RTIGRGLAIGPGPKWEPRLEDPSKLDIGALLAFRFVEGFAMGDAREVDAVAPDSPLPG
                     VAADLGAMLLSHLRTYGERLPVPALMSSFAALMALGLFTFSLRADAAARELLRTGEPP
                     ADLTDGPAPPSPLELYCDFTGNLGSESDRLSRRCVERDLDRVRLAFRDRMTFVVVEQA
                     LPRIAGEQERREALSRSGRLVQLAMLRGHRRVESYAEGRIDDLLADAETSGDATEPDV
                     EFLRRVQAADDSEVDKLLDVIEHVNQAKAVRNAVAWFWSVGALEKTYGLLRGSVRSRR
                     SWRYAPGDELLTALLLAVFVEPDGVTSRATMPLREVLNALAHRFGILIDRPPAFLDGA
                     EAREAATVNREAFTQRLQLLGCFDSLSDDFSVQIVRNPLGDA"
     gene            complement(7730..11527)
                     /locus_tag="VFE39_07265"
     CDS             complement(7730..11527)
                     /locus_tag="VFE39_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6258434.1"
                     /translation="MARLHVLGDWHGPGEEKTARRLAGDLPESWDIIAGRDVPDGMST
                     VDLDLIVVSPRAVYVCEEKAWGRHVVAGDVGWYVNGDRRHNPNSQVQHATRVLAGRIK
                     QRVPGWREAEQLFPHGHRIVRGHVVLSHDRLDLEGAADLGDDVVLRLADTASTLLRLD
                     ATCPDSMTALRPQLMAFLLGLRQRAAAAPPRQIYQYRVEGTPMRRGNAVVYGARNPAG
                     ELVDLICVPVDGAADPVQARRLATREHDALAALAAADRTWRVQGWFDWSGYLVTPIAV
                     ATDGTSLGKLASSRRSGPGGDERVPEEIGIPVVHDAFLALADVHSHGITHRALRLRSI
                     EVTDANRVRFRDFDRAHLPSAVTIAPVLDDVHPSAGFRAPGATLEMFTPADDLYSLAL
                     CLVQWLYGDVTDQPDHDLALRRAQQYPVVGPVLLRCLSRDPGEPFTAAAAAVATAPEP
                     PKPPVVHEQVMEAGALLAGRYRLVRPLGEGAWAVTWLAYDENLDERRTVKHMRAGRVS
                     PEQVKAEYEHANMLRSFHCARMHDRLALPEPGALVQEYVPGETLHQLTTGRPELDREQ
                     ARRIAVDVLRGLADAHERSIYHRDVSPNNIIVRDDGHAVLIDFGLASRAEAAQSAVGS
                     PPFTAPEVWSRRQWSPAADIYSAAASVLHAMLGRLPYAGPGIDERRTLVPPTAEQVER
                     YGRALLDALYRAVEPDPAGRPPDARAFADELVRVSDVVVVPGRRVVNPTVAALRGLYR
                     HSGIGNAGNRGLDDAFAHDTYVATRLDSELLPAIIEGKLDVVVLSGNPGDGKTSFLVK
                     VGSALDRAGAVTLHTDAAGWRRRLGGRTFAAVYDASESHGELSSDDLMCGAIDPGDGD
                     DSAQRTVLLAANDGRIAQFCADHTDRYPEITAALERQRLGAEAAAEARIVLVDLKRRA
                     LALPDLAGPALGANVLASLTVLHRWEVCGGCEVREVCPIRRNAELLRAGQARRAVSEL
                     LLTSHLRRRRRATVRDVRSAFGWLLTGDLSCDTVHDEVEQGLDPGIGRRAFDLAFDAA
                     SGDYLVQEWAELDPAGLPAPAAARAARGHRELVPDLAGLDAGAMTHLKRALFFGAWTV
                     PAARHEVRSYRHLEAFLDALRNPDAGLARILLGVSRVLAFVGYHDEGHLALRDRVFDD
                     PAVRSIVVVKELPAAEFRLVSATSTAPYVESFPDQLELHHTTSGARLRITLDTAELLL
                     RAADGEILGDAASAALRQEIQSFGDRLRLQPARTVRIVDGAGWSMAATVTDAGHIVRG
                     ARR"
     gene            complement(11927..12222)
                     /locus_tag="VFE39_07270"
                     /pseudo
     CDS             complement(11927..12222)
                     /locus_tag="VFE39_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229937.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12280..13071)
                     /locus_tag="VFE39_07275"
     CDS             complement(12280..13071)
                     /locus_tag="VFE39_07275"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6258435.1"
                     /translation="MIYELNHFGIVIRDLEKSLAFYQGALGAEIVFQSVIPSTQTDVV
                     YLQIHGGMIELLHRAAPPADETFGVTHIAFLTDSLDADFAALVDAGYAPLVQPKTAGT
                     GVGRLAFLSDANGARVELLERDLKMRVDPIEHPIVKSFDHYSVIANDLDGALNFYGER
                     LGMKTLTDLRVEKSDLTIKYLHYDYDVLELLHRPTPSTDPIFAHIALRVDDVDSALGT
                     LAAEGIPADPGTPKAAGTGMGRIGIIRDPDGVKIELLDRADLRDV"
     gene            complement(13111..14295)
                     /locus_tag="VFE39_07280"
     CDS             complement(13111..14295)
                     /locus_tag="VFE39_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6258436.1"
                     /translation="MSTVRVGFIGAGFAAHLHVEGLKKVRGHRVELAAVAAGRPASAA
                     DFAARHGVETTYSSYEELLADPSIDVVCVCVPNASHAAISIAAARAGKHVICEKPLTG
                     AFGSSGDLKGAERAHDERERSLASAAGIREAVQAAGVLFLYAENWIYAPAVTKTKRLL
                     ELSKASILDIRAEESHSGSHAARSRRRETAGGGALLMLGSHPIGAALHLKDYESRLRG
                     EPPIRAASVTAEVAALYDTAATHKAGHDWVVSDWDDVETWANTVIGFSDGTKAVITAS
                     FAMLGGVRNMLEVYTTSAAFHANMTPNSSLQVFTPDAAAFGNEYLHEKVESRTGWISV
                     SPDEDWVRGYPQEMQDFVESIVENRQPVSDLDIAIDVVDVIYASYVSAAEGRRVDLAP
                     IV"
     gene            complement(14303..15037)
                     /locus_tag="VFE39_07285"
     CDS             complement(14303..15037)
                     /locus_tag="VFE39_07285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6258437.1"
                     /translation="MIDRAGQVAVVTGGSQGIGAATVAALAARGMTVVAMARDPGRLN
                     DALGALDPGAQARTAAVQVDVSQEVQVEAAVAEVAERFGRIDVLVNSAGVSMNARRRL
                     EHTTTEEWRRLVDTNLTGTYLMCRSALPHLKERGGYILNVLSTGAYRVGEGSSLYAAS
                     KFGARALTEALIEEFRDSPVRTTSVSPGPVDTTIWDHKIEPPAPESRARMMRPSDIAD
                     IFVWLLDRPANLHIPDITVTPWTATG"
     gene            complement(15034..15852)
                     /locus_tag="VFE39_07290"
     CDS             complement(15034..15852)
                     /locus_tag="VFE39_07290"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6258438.1"
                     /translation="MGQYILVYGSGLVLAFFSLAPILWGVSTSLKREGDIYAFPPTWV
                     PAPLTFEHYISVLHNPAILRGFVNTLIIALATTAIALVVGILGAYGFSRYRFPGRDTL
                     LWSVLLTQLFPRVVVIVPFFVTLRNLDLMNTYQGLVLVYLMVIFPIAIWMIKGFFDRL
                     PVEIEEAAVVDGASLWRLLWQVVVPMSRPALAAVAMYSFILAWNEFLFALVFTSGAEK
                     RPLSVGLAFFIDENGIRWGELMAASLLMSVPAVLVFTFSQKLLVRGLSEGAVKG"
     gene            complement(15927..16913)
                     /locus_tag="VFE39_07295"
     CDS             complement(15927..16913)
                     /locus_tag="VFE39_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6258439.1"
                     /translation="MSAPPLGKEADPGAAPPSFRDRVTQKRERRRRILMQKWGPGYLL
                     VTPAIVLILVMMVYPVIQTALFSVSRVQLPTFRTTFVALDNFVSVFTDPATGPLVQRT
                     LIWVIATVALRFVLGFLAALVFNAKVKGTVWLRVLVVLPWTIPSVVAANLWRWILQAD
                     TGVLNQTLRSWGLPEAAANWLGDPHNALFSVIVAYSWAGFPFVMLLILAGMQGIPDEL
                     YEAAKMDGANWWQLFWHITVPSLRPVLIVALVLETVSGINSFDTIMVMTGGGPAGATE
                     IWGIAIYKSGFGDFNFGVASAMSILLFGAAFAFFVVYGIANKGVQKQKGSLR"
     gene            complement(16915..17730)
                     /locus_tag="VFE39_07300"
     CDS             complement(16915..17730)
                     /locus_tag="VFE39_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6258440.1"
                     /translation="MFQLGFSSLACPDYDVDQLIDLATRSGYAGVELRFVRGQVDLTT
                     LEEFSPAKIGETRRRFSDAGLTVACIDTSVRQNALDEETRAAQQEDARANVQIAVGLG
                     AKYIRVFGGPIPGSQDREKTLDAIAVGLGAIAEEAAESGVTAVLESHDDFSTSGSILD
                     LYRRGASDKLQVLWDTLHTFRHGESPEYTWSQIGHLVKHVHMKDSYVATPEGFDFAPT
                     GEGTIPLSEIVAVLTRNGYSGFVDFEWEKAWHPEIEDAEVAVPLFARYMAGVL"
     gene            complement(17771..19042)
                     /locus_tag="VFE39_07305"
     CDS             complement(17771..19042)
                     /locus_tag="VFE39_07305"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET6258441.1"
                     /translation="MAKTLTRLARSAIGVPLLAAALIAAGCSSGSGEAPKEGDASGAL
                     TVWVGSWWEPIVPGIVRDFEAANPGIQLTIEPLPVNGYLDKFTSSALGGNPPDIIDLD
                     STWLATVAAQGLLESLDEHFSDIKQADYAPGIWAASHYQGAMYALPARGEAEVWYYNK
                     TVFDKAGVPYPTDGWTHDDLVRIAQKLTIPGEQWGIGVPADASDPSNVLSLFAPILWY
                     FGGNFLNADNTAAEINSPASVKAIQYWSDFYLKYGASPEGTPNFSTTRDLAPLFQANK
                     LGLIVSSSNTFDTLSQTKGLQWGTVLAPDKINRSGGWTMGIPTGAKNPAAARAFLRWF
                     ADPQNQASHMNRFPARVDARELPPWNDAKYDIFKAASPDSRSVPSVAGWFQMQTAVIS
                     ELQQVLVRQKTPQQAADAAADAINKVIAENH"
     gene            complement(19335..20207)
                     /locus_tag="VFE39_07310"
     CDS             complement(19335..20207)
                     /locus_tag="VFE39_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6258442.1"
                     /translation="MRFALFTGSTPEWTTAEVARKAADQGWRGLEWRVVDQKPSDVPG
                     FWAGSLSTFPLTGIEELVPEIVGTTNGAGLDFAGVAAYVPIGDRGDVERVLAVTAALG
                     AGRVRLTMPKTEAGTDYAELFAQARADAEFASEAAAHRGVQVVIQLHHGTITATSSAA
                     RQLLDGLDPAAIGVMHDLGNTTVEGREGLQTLATGLQILGEYLAHVHIKNVVWRPGEP
                     QEDGTVDWAWSWAPLRTGMADVPGYFRALHSVGYDGWVTLEDFTTELPLEERIADDLA
                     YLTASATAAGYEVK"
     gene            20395..21459
                     /locus_tag="VFE39_07315"
     CDS             20395..21459
                     /locus_tag="VFE39_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6258443.1"
                     /translation="MRHRDGESQATVTISDVARSIGVATSTVSRALTKPGRVSEAMRQ
                     RVLTAANELGYQPNPHARSLTSGRTQSIALVVPDVTNPYFFDLIRGTQAQAKARGYRH
                     VLVDTEESTEMEAATLTELSPSVDGIVVAASRLPDSALLGASERVPLVVVNREIPGVP
                     SVVIDTPMGMVQALDHLVSFGHRSVAYLAGPATSWSSRKRWRAIQQAARRLGITAHSI
                     GPFSPTRAAGAAAADAAIHHGVTACLFFNDLLAIGSLQRFAERGVRVPEDMSVVGCDD
                     IFGADFCHPPLTTLTAPIEHAGRVATDMLLAGLQTAGIDVPAPRQREKLPTHLTIRRS
                     TGPAPGWTGLSTNSTQGNQA"
     gene            21456..22895
                     /gene="uxaC"
                     /locus_tag="VFE39_07320"
     CDS             21456..22895
                     /gene="uxaC"
                     /locus_tag="VFE39_07320"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HET6258444.1"
                     /translation="MTATTKTLLSPHPDRLLPADPGLRTLARTLYESVADARIFSPHG
                     HVDAQVLLGDEPFPDPAALLVTPDHYVTRVMHSLGVGLDQLGLGGPVEPRGVWRTFCT
                     HWDAYLGTASRYWLEVELYDIFGIDVQPSAETADEIFDAIAARLAEPAYRPRALFQQF
                     GIDVLATTDDPVADLSAHAALAADDDFPGAVVPTFRPDRFVDPSADGWQRAVSELGEV
                     TGRDTNRMSEFLDALRDRRRHFIERGGSATDHGTADTGSQPLSASDAASLHSKGIAGT
                     ISAEEATAYRRNMLFEMARMSSEDGLVMQLHPGVLRNHHRPTQRAFGSDTGHDIPTLT
                     GFAAGLRPMLEEFGTHPRFRIVLFTVDETAFARDIAPLAGFYPSVFIGAPWWFLDAPD
                     AISRFRTAVTETAGFYKTAGFVDDTRAFCSIPARHDLSRRVEASYLARLVAEHRISEA
                     DAHRIIHDVVVTLPRHVFTRIDGADPGMA"
     gene            23121..23363
                     /locus_tag="VFE39_07325"
     CDS             23121..23363
                     /locus_tag="VFE39_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258445.1"
                     /translation="MNTVSQILTMVRLVAARRGIAFVPASTRGLGIAGVEYLELSGLA
                     DARGGAAPALDEGLPQPGAVAGARPAGAMTGRPDRC"
     gene            23484..24398
                     /gene="kdgD"
                     /locus_tag="VFE39_07330"
     CDS             23484..24398
                     /gene="kdgD"
                     /locus_tag="VFE39_07330"
                     /EC_number="4.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047448 - 5-dehydro-4-deoxyglucarate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxyglucarate dehydratase"
                     /protein_id="HET6258446.1"
                     /translation="MTSLHPQELASRLPSGLLSFPVTHFDAALEFDEPRYREHLAWQA
                     SFDVAGLFAAGGTGEGFSLTSAEMDRVVRTAVDEIGGRVPVIASATGGTAQAVAQARA
                     AAEAGADGILLMPPYLTEASQAGLIEHASAVCAATGLGVIVYSRANAILTDVTVARLA
                     DRNPTLIGLKDGVGNIEQMTRTYARVGDRLIYIGGLPTAETFALPLLQLGVSTYSSAL
                     FNFLPEFALGFFADVRAQDRTAVYRKLNDFVLPYIDIRDRSKGYAVSIVKAGLTAVGH
                     NPGPVRPPLVDLTESELAELTTLINKVS"
     gene            24400..25719
                     /locus_tag="VFE39_07335"
     CDS             24400..25719
                     /locus_tag="VFE39_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019810892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HET6258447.1"
                     /translation="MSTVASLEVVPVAGHDSMLLNLSGAHGPFFTRNVVVVTDSEGRT
                     GLGEVPGGDAIRRTIEDGGALLVGQPVARYGSLLQQVAAAFADRDAGGRGAQTFDLRT
                     TIHAVTALESALLDLHGQRLGVPVAELLGEGQQRDSVPMLGYLFYVGDRETTDLPYLR
                     EADPRDDWERLRREPALTPAAVVALAEAAQARYGFTDFKLKGGVLAGEEEVAAVRALA
                     TRFPEARITLDPNGGWSLHEAVALCRDLGGVLAYAEDPCGAEGGYSGRETMAEFRRAT
                     GLPTATNMIATDWRQLAAAVRADAIDIPLADPHFWTMRGSVRVAQLCRDFGLTWGSHS
                     NNHFDISLAMLTHVGAAAPDPITALDTHWIWQDGQAITRNPFPISRGRIAVPATPGLG
                     VQLDRDALAAAHELHLEHGLGARDDAVAMQFLVPGWAFDPKRPALMR"
     gene            26220..26429
                     /locus_tag="VFE39_07340"
     CDS             26220..26429
                     /locus_tag="VFE39_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258448.1"
                     /translation="MFITMRSLNRAAVVAGAPLGGLFADAAGLRPALWLGAAGITSTG
                     LLLAMSGFRTASHDDEPPPESAPNP"
     gene            26665..27057
                     /locus_tag="VFE39_07345"
     CDS             26665..27057
                     /locus_tag="VFE39_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET6258449.1"
                     /translation="MSVPRDMPKEFHEMAQTWWPLAVLAVIQLGDAAMCVKPVAFIRQ
                     CLVDVRFPERLWKLLPPLKVAAATGLVAGIWVPSLALLTCAALVCYFVVAIAAHIRAR
                     DLGRNLFLNATAMLALCTATLVFVVEAA"
     gene            complement(27172..28140)
                     /locus_tag="VFE39_07350"
     CDS             complement(27172..28140)
                     /locus_tag="VFE39_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6258450.1"
                     /translation="MSRVVVVGATGHIGSYLVPRLVRAGHEVAALSRGTREPYHHAPE
                     WSRVERVTVDREAEDAAGTFGSRVASLDPDVVVDLVCFTAESAAQLVEALRPTRPLLL
                     HCGTVWVHGPVARVPVTEDEPRKPVGEYGIGKAAIEELLHRETRGGGVPSVVLHPGHI
                     SGPGWQVITPAGNLDPAVWRALALGAPLPLPDLGLGVLHHVHADDVAQAFERALTRPA
                     AIGSSIHVVAEQAMTLRGLAHGVAGWFGREPVIELVDWPTFASLVGAEHAGTTREHVS
                     RSISASIERARTLLGFAPRYTALEALREALDWLVANGHVDLEPSKL"
     gene            complement(28360..29871)
                     /locus_tag="VFE39_07355"
     CDS             complement(28360..29871)
                     /locus_tag="VFE39_07355"
                     /inference="COORDINATES: protein motif:HMM:NF024209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HET6258451.1"
                     /translation="MLVASWVVAIAGIVVGVVLAPHGLSSSALVVAVACSPLMATLAA
                     SIGRTRAGGAAIVVTGFGVAGVGIAITVSTDAMHLFRLNFPLPQVVTNAATVLAPVGL
                     VVVGIVLVVAGLAALFGDRLLGVIAAAIMVVTALLYALALLGLAVQSLAYRGGSIQST
                     PIAAAIIVGVAAMLLVVVVTRWWSLTARTGSAGVDKPTVPSTGPRWAARWTVLGAIVL
                     VAVGTVWAGYSQLGNRLELAALFPDPALAACVRSAVGATDGERVSERELNSVFSLKCN
                     GDLAQDGRIAALGGLDHLPNLVDLDLSGNDVVDLSGLAQVPQLTTLTLTNNGVRDLTP
                     LEGLATLQELGLSGNEVTDLAPLSGLTGLRFLGLSRNAVTGLAPLTPLAGLTELDVSD
                     NHIADVTPLAALSRLDRLLLARNIVADPSVLQGLPALTTLDVSGNRISDAATMAGCPA
                     VDELWIGGNPLTDVRPLQDMPALAGVDLSGSDSTLLTGVEALRSQGVYVGGLA"
     gene            30109..30372
                     /locus_tag="VFE39_07360"
     CDS             30109..30372
                     /locus_tag="VFE39_07360"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HET6258452.1"
                     /translation="MTAHRLASGDLSTRMATTGAAEIGVLEKSVNTMASWLQGSQSEL
                     ANLLEEQAALRRVATLVAEGTPPEKVFPAVNEEVARLLEVARA"
     gene            complement(31161..31412)
                     /locus_tag="VFE39_07365"
     CDS             complement(31161..31412)
                     /locus_tag="VFE39_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258453.1"
                     /translation="MIHVVTALIALVFVLHIVFSLFGANQNSGTVAFVYGAAKIFVFG
                     FGDVFTPSDAKIGLIVNYGLAAIVYLVVGRLIYRTLRHR"
     gene            32320..32751
                     /locus_tag="VFE39_07370"
     CDS             32320..32751
                     /locus_tag="VFE39_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258454.1"
                     /translation="MTTLLLVNLALSAVLAVLFAAFHDTLLDYQVARLALPASADSAA
                     VRASLSAGLWSRALAVIIVGVVYVFLIRRLRRGVRRAYIRVLVISVVSLLGIVYLATS
                     GQYPSWVLVEQVIQALVLLTLLWAVTRPAVRRYFSRSARPA"
     gene            32807..32950
                     /locus_tag="VFE39_07375"
     CDS             32807..32950
                     /locus_tag="VFE39_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258455.1"
                     /translation="MTLASYDLTWLRRIGLIIQIDGRIRYRLTDDGLAFAIFYSKLHY
                     RVL"
     gene            complement(33113..33826)
                     /locus_tag="VFE39_07380"
     CDS             complement(33113..33826)
                     /locus_tag="VFE39_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET6258456.1"
                     /translation="MLPVEESWSSALALVAHPDDLEYGTAAAIARWTDQGKKVAYCLV
                     TSGEAGIETIEPEQCGPLREAEERAGASLVGVDVVEFLGYPDGMLEYGLDLRGAVARA
                     VRRHRPDIIVTGNHHDTWEPGVQNQADHIALGRAVIDGVRDAGNRWVFRELLAEGLQP
                     WRVTQILVADSPLAAHGVNVTDTFDRGVASLEAHGEYLRNLGPNAMSEPREFLDAMAR
                     TAGTRLGCRFAVSFEVINL"
BASE COUNT         5192 a        12107 c        11637 g         5100 t
ORIGIN      
        1 ctggtcacgt gcaacagcta ccgcaacccg gacctgctcg ccgatatggc ccgcacggtc
       61 gaccacatct ccggcggccg gctcgtgctc ggcatcggcg ccggctgggc ccagcgtgac
      121 tatgacgcct acggctaccc gttcgggacg gcaggctccc ggctggccga cctcgcggac
      181 gccctgccgc gcatcaagaa gcggtgggcg cagctgaacc cgccgcccac ccgggagatc
      241 ccgatcctga tcggtggcgg gggcgagaag aagaccctcc gctacacggc cgagcacgcc
      301 accatctggc acggtatcgg cgcgccggcc accatcgcgc acaaggtgca ggtgctcgac
      361 gcgcactgcg ccgacatcgg gcgcgatccg gccgagatcg agcggtcggc caaggtggat
      421 gttccgccgg aggaggtggc cgacgagctc cttgcggcag ggatcacgct cttcaccgtc
      481 agcacctcgg gccccgacta cgatctcggc ctgctcaagt cctggctcgc ctggcgcgac
      541 tcgcgcgagg cctgagcgcg ccgtcgctcg gttgccgtcg ctcggtgccg tctctctcat
      601 cggagatggc gcgccgtcga ccgctcggcg tcctcgcgag gcgcggtccc ccggggacgc
      661 cgaggtcggt cgcgagggcg acgacggact ggtcgatccg ccgcagcatc cgggcggcgg
      721 cgagcaggtc cgtccggtgc gagtgcgaat cgcgggcgac ctcggccgct gcggcgtcga
      781 tgacgccctc gccggaacgg acgtcgttcc gtgaagcggg acttaccagg tcgtgctggg
      841 tgtcaggcgg ttgggtgtgg ctgcccggcg gaatgtggga gtacttcccg aggagctagg
      901 aatcgggcgt gcccctcggg cgccgtctcc cctctcaccc cgccctcggc aaggtcccct
      961 acgaggttcg tgatggacgc cgggttcagg ttctgtggaa ccatgtagat ggatgccggg
     1021 tgctgggacg ctgaagacgt ccggcatccg ctagtcccgc gccatgaaca ccttccgtgc
     1081 cgcggcgtgc tccggcgtcg agacgtacgc ctgccccacc gacaactgct ccagtgcttc
     1141 cgccacgtcc tgcccgcttc gaccccggag gaagcccgcc actgtcttgc tctgcgggta
     1201 gttgcagcgg aaggcgacct tggttcccgc attgcccagg acgtccttat ggaagtcatc
     1261 caccccctgg ctcgcgacca ccaccgcgac gccgtatttg cggccctcct tcatgatctt
     1321 cggaagggtc acgtcgcggg cgaggcggtg cgcctcgtcc agcaccaccg cgaggcgcag
     1381 ctgcttggtc tgcccccacc ggaacatctc ccggtagacc ttgcgcagga cgaaggcacc
     1441 ggccgcgcgc tgcacctgct ccatcagccc atggacgtcg agcaccacgc cctgccggag
     1501 caggtcggcg aacccgcggc cgtgctccgg gtcgacgaac aaaccgaaat cactgagcgg
     1561 gcgcaggcgg gcggccacgt tgcggctgcg actggtggcc tccgccgacg ttacggcctc
     1621 ggcgagctcg tccatggtgg gccagtcgtc agggtgcgac gggcgcggga agccgtgctg
     1681 ctcgtagatt ccgcgcacgc cctcgtagac gacgtttctt tggatctccc ccagcccgca
     1741 gacgtagccg atcacctcgg cgagctccca cgaggcctcg cggtagcggg cgggctcgtc
     1801 gatctcgaac ggcgagatgg gcaggcccat cgacgcgtcg agcaccgcag caccaccggc
     1861 cggggcggcg gccatgtcac cgtggaagtc gatgacgagc gcgggcagcc cctgctccgc
     1921 gaatgagttc agtatgcgac gggtggtcac cgacttgccc tggccgggga tgcccagcac
     1981 gaacaggtgc gggctgcccg aggtgctgat ccgccacggc acgtcctcgg cgtgcgcgtc
     2041 ctggccgagg gtgacctcga cgacctgagg cagcagcgca gcaggaaccg gctgcgcgag
     2101 cacgggatcc gggggcggtg cttcatgcaa cggctgcagc ggtgcgctct gctgcacttc
     2161 ggcccgtcgc ggtgtgaccg acgggagctc cgtcgtcgat tgcacggaac cagctgttgc
     2221 ggccggctcg acggctacgc gcggacgcgg cgacggcctc ggtcgaggcg atgggcgttg
     2281 cggaacgagt tccggcagga tgggcgacac ccgctcgggc tcggcggtgg gcagcgaagt
     2341 ggggggtgac tccggcgccc actcgggctg cgggctcccg ctcgccctcc gcgagacgaa
     2401 ccccgcaggt cccaggtcgg ccgcggtgag cacgtcgatc ttgacgccgc ggtgctcggc
     2461 cgggaaaccg gccttcccac ccaagctgac gacgtagccg cggcggctga tctcgggaac
     2521 cagcgccccg tcctccacct tctcgaccat gcgctccacc tcgccgcgcc tgcggtcgtc
     2581 gagcaggccc atcgcgagcg cgcggtcggc gtggtgacgc aggattccgc cgagacgagc
     2641 acgctgcagc tcggcgtcga tgcggggcgg gtcggtgcgg aagaacgtgt cgtgcagcat
     2701 cgtgactgtg gcgtcgagtt ggtcgacgat gtcgtcgacg agtgcggccg ggagcgcggc
     2761 tgcccgccgt gacttgacct ccacgcactc catcctcagg gtgcgagcgg tggtcctgac
     2821 gaggatcacg tcgcagcgcc gcgccccggc gtcaccccgg gtgtctccga acacctcctg
     2881 atgagcatcg acggggacca ccaggcaacc gtcgagctcg ccgcggccgc gcaggtgtgc
     2941 caccagcgcc gcgaggctga cggcttcggt ggccaggctg gagcgcccga cgagccgcag
     3001 cgcgagcctc ccggacacga cctgcaggtg gtcgagcacg acgcggacgg actcgtccac
     3061 ggcagccagg tcgagccctg tcatcgcatc ggcgagcagc cgttgcacgt cgccgcggtg
     3121 ggtggtggtg acggtgagcc gcggtccgag cccctccagg aagtcggggg cgtagtcgag
     3181 gacgtacggc ggtgcatcgg agcccgacgc ggtggcgaac aggtcgatgc ccaggttgcg
     3241 gtcgagcgtg accacccagt cggcgcgctc gtgcgccgcc ctgagcgacg ccagctcacc
     3301 ggggccgagc cgcgccgtca gagccagcgg tccgcccggg tagccgagcg cgatggccaa
     3361 ggcggcctgg tagctgcggt gcgcttcgac ggcatcggcc gcgccgtccc tgttgcgggc
     3421 ccgaacggct ggggctgtcc gccacacggg cgtcggtgcc gtcacccgct gggtctgcgc
     3481 cggcgtgagg aggttccgga acgacgtcgc ggcgtcgggt accgcgacgg ccgccacgcc
     3541 ggcggcgtcc tcggcgcggg tgatggcgag atccgcgatc accccgatgt gggccgcgtc
     3601 cagatcggcg gcgagccgat cgtgtgaccg gacggtcagt cccagcggcg gggtcaggtg
     3661 cgagcgggcg ccgcgaacct gctgggtcgc caccgcccgc tggagatcgg tcagcgccgg
     3721 caccgggtcg gtgaacgacg gcctgcggct gtacgccgtc agctcgaggc gagccggggc
     3781 ggctgcgagt tcctcgtcag tgtcgggctc gcgcaacacc gattcgtcca gcgcccgggc
     3841 cagcagctcg ccggacccga cgttgtaggc gaggatccgc agcgcgtcct ggccggggtt
     3901 gaccgaccgg tacgcgctga tccgctccgc gacggtcgac ggcggcaggt cgggcgggac
     3961 gtcgcgccgg tcgaggccca ggacctcgaa caccgcctgc acggcggcgc ccggctcggc
     4021 ctccgccgtg tgcaggtaca ccccggtgcc gagcgtggct tcgcgaacat agacgaacgg
     4081 ggacccgtcc acgccgtaca tcgcgaaggg caggttggcc ggggtgaccc ggcgggtgag
     4141 ccgagcgtcc accgactgcc ggcgcttggc cgtcgaccgg ccgagggagg ccagctcgcc
     4201 ggcccatctg gtcagtgtcg cgtcgtactc cgcggcccac gcgagcctca agggctgcaa
     4261 cggcagcgcg atcatcgcgg cgatcggatc gtggccgacg gtgtcgacgc tcagtcgcac
     4321 cgtgtccagg gcgagcagtg ccccgcggct ctcgtcgtcc gcggcggagt ccagtgcccg
     4381 ccggtaggcc tggcagtagg cctgcacctc ctggcgcagc tcgtcgtccc aggcgagcgt
     4441 ctccaccgcg aaccggggac ggcgcgcgtc aagccggtcg aagagccggc gccggcgctc
     4501 cgcgaacgcg ggcggcaggt tgccggccag cggctcaagt gcctcggggt cgagcacctc
     4561 gccgagcctg ccgtcggcct cccaggcgat ggcccggccg cgtccctgga gcagcttgtc
     4621 ctggaggctg gtcagcgcgg ggctcagcgc gagcagagcg gtgcgccgac ccccgaggcg
     4681 cacggagaag gcaccaccgt tccagctcgg cgcatcctcg cgcaggtcgt cctgcccttc
     4741 cagggccgcg tccagacgag cctgggcgag cgagggggtg gacgcccggc gcgtcggccc
     4801 gccgaccggg tcctcctccc accggactcc gaactgctgg ctctcgtcga cggcctcggc
     4861 gccgctgcgc agcagcaccg gctggccgtc cgcgtcgatc gcggtcagcc gcaccacgag
     4921 gagggcgccc tcggtgaggt cctcctcggc gaagtcgatg cggacagtgg cgcggcgctt
     4981 gtcgcccttg accgtctgcc gcgcgaccgg ctcggtgtcg ggatctcgca ggtcgacggg
     5041 cgggagtgcc tcgagcaacc agcggtcgat cgcgtcggtc ttcggggggt cggtgcgcca
     5101 gaccacggtc accgtgcccg gcgtgtccgg tccggtctcg acgaagggca ggtccccggg
     5161 gctctcctgg ctgagccgcg tgccggtgcg cagcgtgccg tcctccttga ggaacgggtc
     5221 gaggcggacg ctgccgacct cgacgggccg ccgctccacg agcggccact tctcgaacgt
     5281 cagctcgtgg ggtggctgag ccagcggggc tgcccacgcc ggagggtcgg agaggtcgac
     5341 gtccgggcgc gagaggtagc gcgcgatgcg gtcgcgggcg tggccctcct gcagctccgc
     5401 gacggtgagc cgatcggtca cggacgccac cgcgcggctc ggccgcgaga tggcacgagc
     5461 acaggcggcg ttgcgggaga ggcgcccgag caggtcgggc ccacccaggt ccggcacgag
     5521 gcccaccatc cacaacgccg cgcccgcggt gtcccaggac gggcctccga cgacggcggc
     5581 cacgtagcgc gcccacgcct cgaccggcct cgtccgcccc agttcccggg ccacctggcg
     5641 cacgccggcc tgcaggtcgg tgtcggcgat cgtcgccacg agcagctcag cggccgcccc
     5701 cagcagaagg gtcacgtcga tccgggcgaa gctgttgtcc agcgagctgg cggccgaccc
     5761 gctgccgaca ggcaccatga gcacgagctg gcgttccttg cggttgcgca gctcgaccgc
     5821 ccgctcggcg gggatcgaca gcttgccgtc agcctccccg ggctggtcca cgaggacgtg
     5881 gacgtcggcg acgccgctcg gcaaccggtc gcggagggtc tccacgagct gggcggcatc
     5941 gcctcggcgg atgctgtcga tacgcacgca gtgccccgcg ccgtggtccc gcagtgcgtc
     6001 ccccacctgg cccgcgatgg cctccaggac caagtcgggc agggcgcagg cgagcgggtc
     6061 aggggtgagc atgtcgggca tcaggcgtct ccgagggggt tgcggacgat ctgcacggag
     6121 aagtcgtcgg acaggctgtc gaaacagccg agcagctgca ggcgctgcgt gaacgcctcg
     6181 cggttcaccg tggccgcctc gcgggcttcc gcgccgtcga ggaaggccgg cgggcgatcg
     6241 atcaggatgc cgaagcggtg ggcgagcgcg ttgaggacct cccgcagcgg catggtcgcg
     6301 cggctggtca cgccgtcggg ctcgacgaag acggccagca gcagtgctgt gagcagttcg
     6361 tcacccgggg cgtagcgcca cgaccggcga ctgcgcacgg atccccgcag caggccgtag
     6421 gtcttctcca aagcgccgac ggaccagaac caggccactg cgttgcggac ggccttcgcc
     6481 tggttgacgt gctcgatcac atcaagcagc ttgtcgacct cgctgtcgtc ggccgcctgc
     6541 acgcggcgca ggaactccac atccggctct gttgcgtcgc ccgaagtctc ggcgtcggcg
     6601 agaagatcgt cgatgcgccc ctctgcgtag gactccacgc gtcggtgccc gcgcaacatc
     6661 gccagctgaa cgaggcggcc ggagcgggac agggcctccc gccgctcctg ctcaccggcg
     6721 atccggggca gggcctgttc caccaccacg aacgtcatcc ggtcgcggaa agccagccgc
     6781 acccggtcga ggtcgcgctc gacgcagcgc cgcgacagcc ggtccgactc cgacccgagg
     6841 ttgccggtga aatcgcagta cagctccagc ggcgacgggg gcgccggtcc gtcggtcagg
     6901 tcggcgggcg gttcgccggt gcgcagcagc tcgcgggccg ccgcatcagc gcgcaggctg
     6961 aacgtgaaca ggcccagcgc catgagcgcg gcgaagctgg acatgagtgc gggcacgggc
     7021 agccgctcac cgtaggtccg caggtgggac agcagcatgg cgccgagatc ggcggccacc
     7081 cccggcagcg ggctgtcggg agcgacggcg tcgacctcgc gggcgtcgcc catggcgaaa
     7141 ccctcgacga agcggaacgc gagcagcgcg ccgatgtcga gcttcgatgg atcctccagc
     7201 cgcggctccc acttcgggcc cggcccgatg gcgaggccgc gcccgatcgt gcgggtgacg
     7261 agctcacgta gcgccgccgc ggttccccca gatccgccac cgggcgcttc gcgatcgatc
     7321 gcctcgagga gcagccggta cacggtgtcg tcgatgccgc ggtggcgctg ccgttccttc
     7381 ggaaggccgg ctcggtaggc ggccagggtc agcggtgcta ctgccagcat ctgctccccc
     7441 gtcttggccc ggcccactgc gcccatgtcg accacgcacg agcgcagcca gccgtcgatc
     7501 agtgcctggt cgcgggggtc gtcgaacccc tcgaccgtgc cgctggccag gagggcccgg
     7561 cggtagccgt cgtagtcgcg ggcatcggtg cgcgaccggc tggaccgccc gcccttgacg
     7621 cacatctcga acagctgcgg cagcacgcga tctgtatcgg tgtcgagcag ggagaacacc
     7681 gtcaagggac ggtactggta cgtcctcagc ggtgtcggca gggagatcgt catcgccggg
     7741 ctcctcggac gatgtggccg gcatcggtca ccgtggcggc catcgaccat cccgcgccgt
     7801 cgacgatgcg cacggttcga gccggttgca ggcgcagccg gtcaccgaag ctctggatct
     7861 cttgacgcag cgccgccgac gccgcatcgc ccaggatctc gccgtcggca gcacgcagca
     7921 gtagttccgc ggtgtccaat gtgatccgaa ggcgcgcacc ggacgtggtg tggtgcagct
     7981 ccagctgatc cgggaaggac tcgacgtacg gcgcggtcga tgtggcgctg accagccgga
     8041 actcagcagc ggggagttcc ttgaccacaa cgatcgaccg aacggcagga tcgtcgaaca
     8101 cgcggtcgcg caacgcgaga tggccctcgt cgtggtagcc gacgaacgcc agcacccgcg
     8161 acaccccgag caggatgcgc gccagtccgg catccgggtt gcgcagcgcg tccaggaacg
     8221 cctccaggtg gcggtagctg cgcacctcgt ggcgggctgc gggcacggtc cacgcgccga
     8281 agaacagggc gcgcttgagg tgcgtcattg ccccggcgtc cagtccggcg aggtccggga
     8341 cgagctcgcg gtggccccgg gccgcccggg ccgcagcagg agccggcaag ccggcggggt
     8401 cgagctcagc ccactcctgg acgaggtagt cgccgctggc ggcgtcgaag gcgaggtcga
     8461 acgcgcgacg accgatgccg gggtcgaggc cctgctccac ctcgtcgtgg acggtgtcgc
     8521 aggacaggtc accggtgagc agccagccga aggccgaccg gacgtcacgc acggtggccc
     8581 ggcgccggcg ccgcagatgg ctcgtgagca gcagctcgga caccgcccgc cgggcctgcc
     8641 cggcgcgcag cagctcggcg ttgcggcgga tcgggcacac ctcgcgtacc tcgcagccac
     8701 cgcacacctc ccaacggtgg agcacggtga gcgaggcgag gacgttcgct ccgagggccg
     8761 ggccggcgag gtccggcagt gccagggcgc gacgcttgag atcgacgagc acgatgcgcg
     8821 cctccgccgc tgcctccgca cccagtcgct gccgctcgag agcagcggtg atctccgggt
     8881 agcggtcggt gtggtcggcg cagaactgcg cgatgcgacc gtcgttggca gccaggagca
     8941 cggtgcgctg agcagaatcg tcgccgtcgc cggggtcgat ggcaccgcac atcaggtcat
     9001 cggagctcag ctcaccgtgc gactccgacg cgtcgtacac cgcggcgaac gtgcggccgc
     9061 cgagacgccg gcgccagccc gcagcgtcgg tgtgcagcgt gaccgccccc gcccggtcga
     9121 gcgcggaccc gaccttcacc aggaaggagg tcttgccgtc gcccgggtta cccgacagga
     9181 cgacgacgtc cagcttcccc tcgatgatcg cggggagcag ctcgctgtcc agccgggtcg
     9241 cgacgtaggt gtcgtgcgcg aaggcgtcgt cgaggccgcg gttgccggcg ttgccgatac
     9301 cgctgtgccg gtagagcccg cgcagggcgg ccacggtcgg gttcaccacc cgtcgcccgg
     9361 gcacgaccac gacgtcgctg acgcggacga gctcgtcggc gaaggcccgg gcgtccgggg
     9421 gccggcccgc cggatcgggc tcgacggcgc ggtagagggc gtcgagcaga gcgcggccgt
     9481 accgctcgac ctgctcagcg gtgggaggaa ccagcgtccg gcgttcgtcg atgccggggc
     9541 cggcgtacgg gagccggccg agcatggcgt gcagcacgga agcggcggcg ctgtagatgt
     9601 cggccgcagg cgaccactgc cgccgcgacc acacctccgg ggccgtgaac ggtggtgatc
     9661 ccacagctga ctgcgcggcc tctgcccgcg acgccagccc gaagtcgatc agcacggcgt
     9721 ggccgtcgtc gcggacgatg atgttgttgg ggctgacatc gcggtggtag atcgaccgct
     9781 cgtgggcatc agccaggccg cgcaacacgt ccacggcgat ccgtctggcc tgctcccgat
     9841 cgagctcggg ccggcccgtc gtgagctggt gcagcgtctc cccgggtacg tactcctgca
     9901 cgagcgcgcc gggttcgggc agggcgagcc ggtcgtgcat gcgggcgcag tggaagctgc
     9961 gcagcatgtt ggcgtgctcg tactcggcct tgacctgctc cgggctgacg cggcccgcgc
    10021 gcatgtgctt gacggtgcgg cgctcgtcga ggttctcgtc gtacgccagc caggtgacgg
    10081 cccacgctcc ctccccgagc ggacgcacca gccggtagcg cccggcgagc agcgcaccgg
    10141 cctccatgac ctgctcgtgc accacaggcg gtttcggcgg ctccggagcc gtagccacag
    10201 cagccgcagc agcggtgaag ggctcccccg gatcccggga gaggcagcgc agcaggaccg
    10261 ggcccaccac cgggtactgc tgcgcccgcc gcagcgcgag gtcgtgatcg ggctggtccg
    10321 tgacgtcccc gtacagccac tgaacgaggc agagtgcaag gctgtagagg tcgtcggccg
    10381 gagtgaacat ctccagcgtc gcccccggag cgcggaaccc ggcggaggga tggacgtcgt
    10441 ccagcaccgg tgcgatggtg accgcagacg gcaggtgcgc ccggtcgaag tcgcggaacc
    10501 gcacccggtt ggcgtcggtc acctcgatgc tgcgcagccg gagggcgcga tgcgtgatgc
    10561 cgtgggagtg cacgtcggcc agcgcgagga aggcgtcgtg caccacgggg atgccgatct
    10621 cctccggcac ccgctcgtcc ccgcccggcc ccgagcggcg cgaagaagca agcttgccca
    10681 ggctcgtgcc gtccgtcgcc accgcgatcg gcgtaacgag gtagccgctc cagtcgaacc
    10741 acccctgcac ccgccaggtc ctgtccgccg cggccaacgc cgcgagcgcg tcgtgctccc
    10801 gggtggcgag ccgccgtgcc tgtaccggat ctgcggcccc gtcgaccgga acgcagatga
    10861 ggtccacgag ctcgcccgct gggttgcgcg ccccgtagac gacggcgttg ccccgacgca
    10921 tcggcgttcc ttcgacgcgg tactggtaga tctgacgcgg cggcgcggcc gcagcacgct
    10981 gccgaaggcc cagcaggaac gccatgagct gcgggcgcag agccgtcatg gagtcgggac
    11041 aggtggcgtc gagacgcagg agcgtggagg cggtgtcggc gagtcgcagc acgacgtcgt
    11101 cgccgagatc ggccgcaccc tccaggtcca ggcgatcatg ggagagcacg acatgacccc
    11161 ggacgatgcg atgcccgtgg gggaacagct gctccgcctc acgccatccg ggtacccgct
    11221 gcttgatcct ccccgcgaga acgcgggtgg cgtgctggac ctggctgttg gggttgtgcc
    11281 gacggtcgcc gttgacgtac cagccgacat cgcccgcgac gacgtgtcgg ccccacgcct
    11341 tctcctcgca gacgtagacg gcgcgcgggg agaccacgat caggtcgaga tccacggtgc
    11401 tcatcccgtc cgggacgtcc cggccggcga tgatgtccca gctctcgggc agatcaccgg
    11461 cgagcctgcg ggccgtcttc tcctcgccgg gaccgtgcca gtcaccgaga acatgcagcc
    11521 gtgccacgac gtcattcccc ccaagatcaa ccagcgcgag gagctagcga cgccagcagc
    11581 ggcgcagccg ctcttcacct gtcatcgact gccgctgcgc tggagtgttc tcgcccgacg
    11641 catggtcatc gacagctggc gccctcttat tacgtcggag cccgtcctct gggatgtgac
    11701 tcgacgtagt cggccgtgaa tgtcggctga cgtccgctga cctgctgctt cgtcgccggc
    11761 gggagcccgg gatgatccgc atgatccacc ggtggtgcgc gcgatccgac acgcacggcc
    11821 gacgatcagg cacgctcggc ggcgaacggc cgccggtgcc gaaggccagg gcggcgaagt
    11881 gttcgccgat gtggcggtgt gtggcggcgt ccgggtggag ctggtgcaga cgcatcaggt
    11941 cggtgttgac caggttgatg ccgatcttga cgctgatcag gtccgcgggg gtgtcccgca
    12001 tggcgcgggc ggtgaacgca tcgagcaggg cgctgccgcc cagaccgagg ttgatcagct
    12061 ccacgccgcc gagggaggcg gccagcgccg gccaggtcgt gggaacacca gccgtacgcc
    12121 ggagggctgg gcctccgcca tggccagctg cccgtcggcg cattgtgcgc gggcccgtgc
    12181 gggcagccgg tgcggcagca ccccgtgctc ggtgcgctcc acgtcgagcg cgccgcggct
    12241 gtgggggcga gctgattctc gcccccatcg tggacccgct cagacgtccc ggaggtcggc
    12301 ccggtcgagc agctcgatct tcacaccgtc cgggtcgcgg atgatgccga tgcggcccat
    12361 acccgtgcct gccgccttcg gcgttcccgg gtcggccggg atcccctcgg ccgcgagcgt
    12421 tccgagtgcg gagtcgacgt cgtccacgcg gagggcgatg tgcgcgaaga tcgggtccgt
    12481 cgatggcgtc gggcgatgca ggagctcgag cacgtcgtag tcgtagtgca ggtacttgat
    12541 cgtgaggtcc gacttctcca cccggaggtc ggtgagggtc ttcatcccca gccgctcgcc
    12601 gtagaagttc agcgccccgt cgaggtcgtt cgcgatcaca gagtagtggt cgaacgactt
    12661 cacgatcggg tgctcgatgg gatccacgcg catcttcaga tcgcgctcga ggagctccac
    12721 gcgcgcaccg ttcgcgtcgc tcaggaaggc gagccggccg actcccgtgc cggccgtttt
    12781 cggctggacc aggggcgcgt atccggcgtc caccagtgct gcgaagtccg catcgaggct
    12841 gtcggtgagg aacgcgatgt gggtgacgcc gaacgtctcg tcggcgggcg gggcggcgcg
    12901 gtgcagcagc tcgatcatcc cgccgtggat ctgcaggtag accacgtcgg tctgcgtgga
    12961 cgggatcacg ctctggaaca cgatctccgc gccgagggcg ccctggtaga aggcgagcga
    13021 cttctccaga tcacggatca cgatgccgaa atggttgagc tcgtagatca ttctcgtccc
    13081 tctcgatcgt tctttttcaa ccgggattgg tcagacgatg ggcgcgaggt cgacgcgccg
    13141 gccttccgcc gcggagacgt aactggcgta aatgacgtcc acgacgtcga ttgcgatgtc
    13201 gaggtccgag accggctgcc ggttttcgac tatgctctca acgaaatcct gcatttcctg
    13261 cgggtatccg cgcacccagt cctcgtccgg tgagaccgag atccagcccg tgcggctctc
    13321 gaccttctcg tgcaggtact cgttcccgaa ggccgccgcg tcgggggtga acacctgcag
    13381 gctcgaatta ggcgtcatgt tcgcgtggaa cgccgcgctg gtggtgtaca cctccagcat
    13441 gttccgcact ccgccgagca tcgcgaacga cgcggtgatc acggccttgg tgccgtcgct
    13501 gaagccgatc accgtgttgg cccaggtctc gacgtcgtcc cagtccgaga ccacccagtc
    13561 gtgcccggcc ttgtgggtgg cggccgtgtc gtagagggcg gcgacctccg cggtcaccga
    13621 cgcggcgcgg atcggcggct cgccgcgcaa ccgcgactcg tagtccttga ggtggagggc
    13681 cgccccgatg gggtgcgaac cgagcatgag cagggcaccg ccacctgccg tctcccggcg
    13741 ccgggagcgg gcggcgtgag agccgctgtg gctctcctcg gcgcggatgt ccagaatgga
    13801 ggccttcgac agttccagca gccgcttggt cttcgtgacc gcgggggcgt agatccagtt
    13861 ctcggcgtag aggaagagca ctcccgccgc ctgcacggct tccctgatcc ccgccgcgga
    13921 ggccaggctc cgctcgcgct cgtcgtgagc gcgctcggcc cccttgaggt cgccgctgct
    13981 gccgaaggca ccggtcagcg gcttctcgca gatgacgtgc ttgccggccc gtgcggcggc
    14041 aatcgagatc gccgcgtgcg aggcgttggg cacgcagaca cacacgacgt cgatcgacgg
    14101 atcggcaagg agctcctcgt agctcgagta cgtggtctcc accccgtgcc tcgctgcgaa
    14161 gtcggcggcc gaggcgggac ggccggcggc gaccgcggcg agctcaacgc ggtgtccgcg
    14221 gaccttcttg aggccctcca cgtgcaggtg ggccgcgaat cctgcgccga tgaaccctac
    14281 gcggactgta ctcatctgct cctcatccag tggccgtcca cggagtgacc gtgatgtcgg
    14341 ggatgtgaag gttcgcgggc cggtcgagca gccatacgaa gatgtccgcg atgtcggagg
    14401 gacgcatcat gcgcgcccgg gactccggcg ccggcggctc gatcttgtgg tcccagatgg
    14461 tggtgtccac cgggccgggg ctgacggacg tcgtgcgcac cggtgagtcg cgaaactcct
    14521 cgatgagcgc ctcagtgagg gcccttgctc cgaacttcga cgcggcgtac aggctcgaac
    14581 cctcgcccac ccgatacgcc ccggttgaca gcacgttcag gatgtagccg ccgcgctcct
    14641 tcaggtgcgg cagggccgag cggcacatca ggtaggtccc ggtgaggttc gtgtcgacga
    14701 gcctgcgcca ctcctcggtg gtggtgtgct ccagccgccg gcgggcgttc atgctcacgc
    14761 ccgcgctgtt gacgagcacg tcgatccgcc cgaaccgctc ggcgacctcc gcgaccgccg
    14821 cctcgacctg cacctcctgg ctcacgtcca cctgcacggc tgcggtccgc gcctgtgcgc
    14881 cggggtcgag ggcgcccagg gcgtcgttca gccgcccggg atcgcgggcc atcgcgacga
    14941 cggtcatccc ccgcgctgcg agcgcggcga ccgtcgccgc cccgatcccc tggctccccc
    15001 cggtgaccac ggccacctga cctgcgcgat cgatcatccc ttgaccgctc cttcgctcag
    15061 cccgcggacg agcagcttct gcgagaacgt gaacacgagc accgcgggca cgctcatcag
    15121 caacgacgcc gccatcagtt cgccccagcg gatgccgttc tcgtcgatga agaacgcgag
    15181 accgaccgag agtggacgct tctcggcgcc gctggtgaac accagggcga acaggaactc
    15241 gttccacgcg aggatgaacg agtacatcgc gacggccgcg agtgccgggc gcgacatggg
    15301 gacgacgacc tgccacagca ggcgccacag cgaggcgccg tcgacgacag ccgcctcctc
    15361 gatctcgacc ggcagccggt cgaagaagcc cttgatcatc cagatggcga tcgggaagat
    15421 caccatcagg tagaccagca cgagcccctg gtaggtgttc atcaggtcga ggttccgcag
    15481 ggtcacgaag aacggcacga tcacgaccac gcgcgggaag agctgtgtca acagcaccga
    15541 ccacaggagg gtgtcgcgcc ccgggaagcg gtaacgcgag aacccgtatg caccaaggat
    15601 gcccaccacc agcgcgatcg ccgtggtcgc cagcgcgatg atcagggtgt tgacgaagcc
    15661 gcgcaggatc gccgggttgt gcagcaccga gatgtagtgc tcgaacgtca gtggcgcggg
    15721 gacccaggta ggcgggaagg cgtagatgtc gccctctcgc ttcagggagg tgctcacccc
    15781 ccagaggatc ggcgcgaggc tgaagaaggc cagcacgagg ccggagccgt acaccaggat
    15841 gtactgcccc attgcgtgcg cgcgtggcgc gtgccatcgc ctgggtggct tcgcggtgcc
    15901 ccctccggag ccccgctcga ggaggatcat cgcagcgatc ccttctgctt ctgcacgccc
    15961 ttgttcgcga tcccgtagac cacgaagaag gcgaatgccg caccgaagag caggatgctc
    16021 atggcagaag cgacgccgaa gttgaagtca ccgaatccgg acttgtagat cgcgatgccc
    16081 cagatctccg tcgcacccgc gggaccaccg ccggtcatca ccatgatggt gtcgaaggag
    16141 ttgatgccac tcaccgtttc gagcacgaga gccacgatca gcaccggacg cagggacggc
    16201 acggtgatgt gccagaacag ctgccaccag ttcgctccgt ccatcttcgc ggcttcgtac
    16261 agctcgtcgg ggatcccctg catcccggcg aggatcagga gcatcacgaa cgggaacccg
    16321 gcccaggagt acgcgacgat caccgagaag agcgcgttgt gcgggtcgcc cagccagttg
    16381 gccgcggcct ccggtaggcc ccatgaccgc agcgtctggt tgagcacccc ggtgtcggcc
    16441 tgcaggatcc agcgccagag gttcgcggcg acgacggagg ggatggtcca cggcaggacc
    16501 accagcaccc gcaaccagac cgtgcccttg accttcgcgt tgaagacgag cgcggcgagg
    16561 aaaccgagga cgaaccgcag tgcgaccgtg gcgatgaccc agatgagcgt gcgctgcacg
    16621 agcgggccgg tggcggggtc cgtgaagacg ctgacgaagt tgtcgagagc gacgaaggtg
    16681 gtcctgaagg tcggcagctg cacccgcgac acgctgaaca gcgcggtctg gatgaccggg
    16741 tagaccatca tgaccaggat cagcacgatc gccggcgtca cgagcaggta gcccggaccc
    16801 cacttctgca tgagtatccg gcggcggcgc tcgcgtttct gcgtgacgcg gtcccggaaa
    16861 ctgggcgggg cggcgcccgg gtcggcttcc ttccccagtg gaggagccga catctcacag
    16921 aactcccgcc atgtaccggg cgaacagggg aaccgcgacc tcggcgtcct cgatctccgg
    16981 gtgccaggcc ttctcccact cgaagtcgac gaagcccgag taaccgttcc tcgtcaggac
    17041 ggcgacgatc tcggaaagtg ggatggtgcc ctcgccggtg ggggcgaagt cgaagccctc
    17101 cggcgtcgcg acgtacgagt ccttcatgtg gacgtgcttg accagatgcc cgatctgcga
    17161 ccaggtgtac tccgggctct cgccgtgccg gaaggtgtgc agcgtgtccc acagcacctg
    17221 cagcttgtcg ctcgcgccgc ggcggtagag gtccaggatg ctccccgacg tcgagaagtc
    17281 gtcgtgcgat tcgaggacgg cggtgacgcc gctctcggcg gcctcctccg cgatggcccc
    17341 cagcccgacg gcgatcgcgt cgagggtctt ctcccggtcc tgggagccgg ggatgggacc
    17401 cccgaagacc cggatgtact tcgccccgag cccgaccgcg atctgcacgt tggcccgcgc
    17461 gtcctcctgc tgggccgcac gcgtctcctc gtcgagcgcg ttctgccgga cgctcgtgtc
    17521 gatgcaggcg acggtgaggc cggcgtcgga gaagcggcgc cgggtctcgc cgatcttcgc
    17581 cggcgagaac tcctccagtg tcgtcagatc cacctgacct cggacgaacc ggagttcgac
    17641 gcccgcgtag ccgctcctcg tcgccagatc gatgagctgg tcgacgtcgt agtccgggca
    17701 ggcaagtgaa ctgaatccga gctggaacac ggcggcgctc ctccttgtgg gctgattccc
    17761 gaatccggta tcagtggttt tccgcgataa ccttgttgat cgcgtcggca gccgcgtccg
    17821 ctgcctgctg cggggtcttc tggcgcacga gcacctgctg cagttcgctg atcactgcgg
    17881 tctgcatctg gaaccatccg gcgaccgagg gcaccgaccg ggagtccggg ctcgctgcct
    17941 tgaagatgtc gtacttcgcg tcgttccacg gcggcagttc gcgggcgtcg acgcgagcgg
    18001 ggaagcggtt catgtgcgac gcctggttct gcggatcggc gaaccaccgc aggaacgccc
    18061 gggcggccgc agggttcttc gcaccggtcg ggatacccat cgtccaaccg ccactgcggt
    18121 tgatcttgtc cggggcgagg accgtgcccc actggagccc cttggtctgc gagagcgtgt
    18181 cgaaggtgtt cgacgagctc acgatcaggc ccagcttgtt cgcctggaac agtggggcga
    18241 ggtcgcgcgt ggtcgagaag ttcggcgtcc cctcaggcga ggcaccgtac ttgaggtaga
    18301 agtcggacca gtactggatg gccttgacgc tcgccgggct gttgatctcc gccgcggtgt
    18361 tgtccgcatt gagaaagttt ccgccgaagt accacaggat cggcgcgaac agtgagagca
    18421 cgttggacgg gtcgctcgcg tcggccggca ccccgatccc ccactgctcg cccgggatcg
    18481 tcagcttctg ggcgatccgc acgaggtcgt cgtgggtcca gccgtccgtc gggtagggca
    18541 cgcccgcctt gtcgaagacc gtcttgttgt agtaccagac ctcggcctca ccgcgggcgg
    18601 ggagggcgta catcgcgccc tggtagtggc tcgccgccca gatccccggc gcgtagtcgg
    18661 cctgcttgat gtccgagaag tgctcgtcga gcgactccag gaggccctgc gcggccaccg
    18721 tggccagcca ggtggagtcc aggtcgatga tgtcgggcgg gttcccgccg agagcggacg
    18781 aggtgaactt gtcgaggtag ccgttcaccg ggaggggttc gatggtgagc tggatccccg
    18841 ggttggctgc ctcgaagtcc cggacgatcc cggggacgat gggctcccac cagctgccca
    18901 cccacacggt gagagcgccg ctcgcgtctc cctctttcgg tgcctcgccg gatccggaac
    18961 tacaccccgc ggcgatgagt gcggccgcga gcagcgggac tccgatcgct gatcgggcca
    19021 gcctcgttag cgtctttgcc atcggctttg tcctgacgat tgtgtggcca cgccggccac
    19081 ctgtgagatg tgttcgtctt gcgtcgactg ccggattgag gcgcgtcggc gtgggcctgc
    19141 gatgatcctg caatctgttg ccgtacccgt caacctgtcg ttctgacaac atcgatcacg
    19201 aaatcatcgg tccgatgaat ggcgtgccgg aactgcggaa gtcgcgatcc ggaacggcgt
    19261 cgatcaagcg gagccggcgc gattccgccg gcgatccccg cctggttctc aggccggcga
    19321 cccctccggg tacgtcattt cacctcgtac cccgcggcgg tggccgaagc ggtgaggtag
    19381 gcgaggtcgt ccgcgatgcg ctcctccagc gggagctccg tcgtgaaatc ctcgagggtg
    19441 acccatccgt cgtaccccac tgagtggagc gcgcggaagt agcccgggac gtcggccatc
    19501 cccgtccgga gcggtgccca gctccacgcc cagtcaacgg tgccgtcctc ctggggctcc
    19561 cccggtctcc agaccacgtt cttgatgtgg acgtgcgcga gatactcacc gaggatctgc
    19621 aggccggtag ccagtgtctg cagcccctcg cgcccctcca ccgtggtgtt gcccaggtcg
    19681 tgcatgacgc cgatcgcggc cgggtcgagc ccgtcaagga gctggcgtgc ggccgaggag
    19741 gtcgcggtga tggtgccgtg gtgcagctgg atcacgacct ggacgccgcg gtgggcggcc
    19801 gcttcactcg cgaactccgc gtccgccctc gcctgggcga agagctcggc gtagtcggtg
    19861 cccgcctccg tcttcggcat cgtcaggcgc acgcggccgg cgccgagggc cgcggtcacc
    19921 gccagaacgc gttccacgtc gccgcgatcc ccgatcggga cgtacgcggc gacaccggcg
    19981 aaatcgagtc cggcgccgtt agtcgtaccg acgatctcgg gtaccagctc ttcgatcccg
    20041 gttagcggga acgtcgagag actgcccgcc cagaatccgg gaacatcgct cggcttctga
    20101 tccacgaccc gccactccag gccgcgccaa ccctgatcag ctgctttcct ggcgacttcc
    20161 gctgtcgtcc actcgggcgt ggacccggtg aaaagtgcga atctcatgcg gcgcgcagct
    20221 cctttgctcg cgggcttcac ttcctcgctc ctgacctgcg gccgggcccc gccgcgccac
    20281 aaacggtagg gcctcggcaa cagcttggca aggggttgcc aatccttgcc atggctgtgc
    20341 cagtatgaga tctttggcca gccacatcgg tttgaccggg aagtggagta ctggatgcgc
    20401 catcgggacg gcgagtcgca agccacggtg acgatctcgg acgtcgcgag atccatcggt
    20461 gtcgcgacgt cgacggtttc gcgtgcgctg acaaagccgg gccgagtgag cgaggcaatg
    20521 cgacaacgag ttctcaccgc cgcgaacgag ctcggttacc agcccaatcc gcacgcccga
    20581 tcactcacct cggggcggac gcagagcatc gccctggtcg ttcccgacgt cacgaacccc
    20641 tacttcttcg acctgatccg cggcacgcag gcccaggcga aggcacgcgg ttaccgtcac
    20701 gtcctcgtcg acaccgagga gtcgacggag atggaggccg ccaccctcac cgagctctca
    20761 ccgtcggtcg acggcatcgt cgtcgcggcg tcccggctcc ccgattccgc gctgctgggg
    20821 gcctccgagc gcgtgccact ggtggtggtg aaccgggaga tcccgggcgt gccgagcgtg
    20881 gtcatcgaca ccccgatggg aatggtccag gcgctcgacc acctcgtgtc cttcgggcac
    20941 cgatccgtcg cctacctcgc cgggcccgcc acgtcgtggt ccagccgcaa acgctggcgg
    21001 gcgatccagc aggcggcccg gcgactgggg atcaccgcgc actcgatcgg gccgttctcc
    21061 cccacgcgag cggcgggggc agcggcagcg gacgccgcga tccaccacgg cgtcacggcc
    21121 tgcctgttct tcaacgacct cctcgcgatt ggctcgctgc agcggttcgc cgaacggggc
    21181 gtccgcgtcc ccgaggacat gagcgtggtc ggctgcgacg acatcttcgg cgccgacttc
    21241 tgccatccac cgctcaccac gctcaccgcg cccatcgagc atgccggtcg cgtggcgacc
    21301 gacatgctgc tcgccggtct ccagacggcg ggcatcgacg tccccgctcc tcggcagcgc
    21361 gagaagctcc cgacgcacct cacgatccgc cgatccaccg ggccggctcc cggctggaca
    21421 gggctctcga ccaactcgac gcaggggaac caggcatgac agcgaccacg aagacgctcc
    21481 tctcccccca ccccgatcgg ctgctgcccg cggaccccgg tctgcgcacg ctcgccagga
    21541 cgctgtacga gtcggtggcc gatgcccgga tcttctcgcc ccacggccac gtggacgccc
    21601 aggtcctcct cggcgacgag cccttccccg accccgcggc gctcctcgtc acaccggacc
    21661 actacgtcac ccgcgtgatg cactcgctcg gtgtcggcct cgaccagctg gggctcggcg
    21721 gtcctgtcga accccggggg gtctggcgca cgttctgcac ccactgggac gcgtacctgg
    21781 gcaccgcctc ccgttactgg ctcgaggtcg agctctacga catcttcggt atcgacgtgc
    21841 agccatccgc cgagaccgcc gacgagatct tcgacgcgat cgcggcgcgc ctggccgaac
    21901 cggcgtaccg gccccgcgcg ctcttccagc agttcgggat agacgtgctc gcgacgaccg
    21961 acgaccccgt cgcggacctg agcgcccacg ccgccctcgc cgccgacgac gatttccccg
    22021 gtgcggtagt cccgacgttc cgtcccgacc gcttcgtcga cccgtccgcc gacggctggc
    22081 agcgagcggt gtccgagctc ggcgaggtga ccggccggga caccaaccgg atgagcgagt
    22141 tcctcgacgc gctccgcgac cggcgcaggc acttcatcga acgcggcggg agcgcgacgg
    22201 accacggcac cgccgacacc ggcagccaac ccctgtcggc ctccgatgcg gcgtccctcc
    22261 actccaaggg gatcgccggc accatcagcg ccgaggaggc gaccgcctac cgccgcaaca
    22321 tgctgttcga gatggcccgg atgtcgagcg aggacgggct ggtcatgcag ctgcacccgg
    22381 gcgtactccg caaccaccac cgccccaccc agcgggcctt cggctcggac accggccacg
    22441 acatccccac gctcaccggc ttcgcggccg ggctccggcc gatgctcgag gagttcggca
    22501 cccacccgcg cttccgcatc gtcctgttca ccgtcgacga gacggccttc gcccgggaca
    22561 tcgcacctct cgccgggttc tacccctccg tcttcatcgg agcgccatgg tggttcctcg
    22621 acgcgcccga cgccatcagc cgcttccgca cggctgtaac tgagacggcc ggcttctaca
    22681 agaccgccgg gttcgtcgac gacacccgcg cgttctgctc catcccagcc cgacacgacc
    22741 tctcccggcg ggtcgaggcg agctacctcg cccggctggt ggccgagcac aggatcagcg
    22801 aggccgacgc ccaccggatc atccacgacg tcgtggtcac gctcccccgg cacgtgttca
    22861 cgcgcattga cggcgcggac ccgggaatgg catgaacctc accaggccac agccgtaagc
    22921 cccggcgata ttcgactccc ggctgctgca ccgcgaggcg atgctcgtcg cggccctacc
    22981 gggcatcggc tcaccggcct cggccgcccg gtcacggcaa gcgacctcgt gcaggagcgg
    23041 ctcatcatgc atttcgcgac gcacgcccgg tacttctacg atttcgtggt cgttctggtg
    23101 ccgatccgca ccagaacgtc gtgaacaccg tgagccagat cctcacgatg gtccggctgg
    23161 tcgcggcccg acgcggaatc gcgttcgtcc cggcgtcgac gagagggctc ggcatcgcag
    23221 gcgtcgagta cctcgagctg agcgggctgg ccgacgcccg cggtggagct gcacctgctc
    23281 tggatgaagg actcccccag cccggcgctg tggcgggtgc tcgaccagct ggagcgatga
    23341 caggacgacc ggaccgatgc tgacccggta tcactcgatg cacatcgcac ttggacaggc
    23401 atcgaacggc gttcccaggg tgacggcgtt ccctccgcgt gtggtgcgcc cggcccgccc
    23461 gtcgacctcg aaggacttgc actgtgacct cgttgcaccc tcaggagctc gcctcaaggc
    23521 tgccatccgg cctgctgtca ttccccgtca cccacttcga cgccgccctc gagttcgacg
    23581 agccgcgcta ccgcgagcac ctcgcatggc aggcgagctt cgacgtcgcc ggcctgttcg
    23641 ccgcgggggg taccggcgag ggcttctccc tcaccagcgc cgagatggac cgcgtcgtgc
    23701 ggacggccgt cgacgagatc ggcgggaggg tgcccgtcat cgcctcggcc acgggcggca
    23761 cggcgcaggc ggtggcccag gcccgcgccg ccgcggaggc cggtgccgac ggcatcctgc
    23821 tgatgccgcc atacctcacc gaggccagcc aggccggcct gatcgagcac gcgagcgccg
    23881 tctgcgcggc taccggcctc ggcgtcatcg tctacagccg tgcgaacgcg atcctgaccg
    23941 acgtcacggt ggcgcggctt gccgaccgca accccaccct gattggcctc aaggacggag
    24001 tcggcaacat cgagcagatg accaggacct acgccagggt cggggatcgt ctgatctaca
    24061 tcggagggtt gccgaccgcc gagacattcg cgttgccgct gctccagctc ggtgtcagca
    24121 cctactcctc ggcgctgttc aacttccttc cagagttcgc gctcgggttc ttcgcggacg
    24181 tgcgcgcgca ggaccggacc gccgtctacc ggaagctcaa cgacttcgtc ctgccctaca
    24241 tcgacatccg cgaccggtcg aagggatacg ccgtgtccat cgtcaaagcg ggcctcaccg
    24301 cggtcggcca taaccccggt ccggtgcgac ccccactggt cgacctgacg gagtccgagc
    24361 tcgccgagct gaccacgttg atcaataagg tctcctgacg tgagcactgt cgcgtccctc
    24421 gaggtggtgc ccgttgcggg gcacgacagc atgctgctga acctcagcgg cgcccatgga
    24481 cccttcttca cgcgcaacgt cgtcgtcgtc accgacagcg agggccggac cgggctcggc
    24541 gaggttcccg gtggcgacgc gatccggcgc acaatcgagg acggcggcgc gctgctcgtc
    24601 gggcagccgg tggcccggta cgggtcactg ctgcagcagg tggccgccgc cttcgccgac
    24661 cgcgacgccg gtgggcgggg cgcccagacg ttcgacctgc gcaccacgat ccacgccgtg
    24721 acggcgctcg aatcggcgct gctcgatctg cacggccagc gcctcggggt gccggtcgcg
    24781 gagctgctcg gcgagggcca gcagcgcgac agcgtgccga tgctcggcta cctgttctac
    24841 gtcggggacc gcgagaccac cgatctcccg tacctccgcg aggccgatcc acgcgatgac
    24901 tgggagcggc tgcgccgcga gccggcgctc acgccggcgg ccgtcgtcgc gctcgcggag
    24961 gcggcacagg cgcggtacgg cttcaccgac ttcaagctga agggcggcgt cctcgcgggc
    25021 gaggaggaag tggccgccgt ccgggcactc gcgacgcgtt tcccagaagc ccggatcacg
    25081 ctcgacccca acggcggctg gtcgcttcac gaggccgtgg cactatgccg cgacctcggc
    25141 ggcgtgctcg cctatgccga ggacccgtgc ggagccgaag gcggctactc ggggcgcgag
    25201 acgatggcgg agttccgccg ggccaccggg ctcccgaccg cgaccaacat gatcgcgacc
    25261 gactggcgac agctggcggc cgcggtgcgg gccgacgcca tcgacatccc gctcgccgac
    25321 ccccacttct ggaccatgcg gggttcggtg cgggtcgcgc agctctgccg cgatttcgga
    25381 ctgacctggg gatcgcattc gaacaaccac ttcgacatct ccctggcgat gctcacacac
    25441 gtcggcgcag cagctccgga cccgatcacc gccctcgaca cccactggat ctggcaggac
    25501 ggccaggcga tcacccggaa cccgttcccg atcagccgcg gccggatcgc ggtgccggcc
    25561 acgcccggcc tcggcgtcca actcgaccgc gacgcactcg ccgctgctca tgagctccat
    25621 ctcgagcacg ggctcggcgc cagggacgac gccgtagcca tgcagttcct cgtgcccggc
    25681 tgggcgttcg accccaagcg gcctgcgctg atgcgatgag cgtgcaggac gccgggcacc
    25741 gttaccccgc gaaccaccat cgctagtgcg gtggccatga acgtccaccg gttcacgaca
    25801 cgccgaccga tgtcccgaga ctgacctggc gctgctccac cctcggtccc gccatcaaca
    25861 cgggatcgag gaggtaggag cgggtggccg aacccgtcag agcacgtcga ctgtccgacc
    25921 acgaaggtca acggctgacc caactcgtcc gccgcggccg cgggaacccg atccgcatcc
    25981 gccgagccac gatcatcatg gcctcggcct cggggacccc cggtgtccgc gatcacccgg
    26041 ctggtcgccg cggacgaaga caccgtccgg gacgtgatcc acgccgtgcc gacccgacgc
    26101 cacgaccccc caccctgtcg gtcctcgccg agctgcgcga gggcaccagt tgggtctaac
    26161 ggcaccccac gctgcggccg ctcgccctct ccacgcacgg ctggttcctg ttcaacgccg
    26221 tgttcatcac catgcgctcg ctcaaccgcg ccgccgtcgt cgccggcgcc ccgctgggcg
    26281 gattgttcgc cgacgccgcg ggcctgcgac ccgcactctg gcttggggcc gcgggaatca
    26341 ctagcacagg gctgctactc gccatgtccg gcttccgcac agccagccac gacgacgagc
    26401 caccacccga aagcgcaccg aacccctgaa cgaccgctta caaggcggtt cctccgcgtc
    26461 gccacgctgg tgccctcgct ctatcggtca gccgagtacc tggcgacctc cgcatcgggt
    26521 gtcgtcggag ccatcgggct cgacctgctc ggcccccgcg ctcatcgccc cactcgccgc
    26581 ggttgttgtc ctcgcgagcg ccggggcagt gcaggcgccc gtccgggacc gtgtcacatc
    26641 gcgggcagcc gcaccgtcca tccggtgtcc gttcctcgtg acatgccgaa ggagttccac
    26701 gagatggcgc aaacctggtg gccgctggcg gtgctggccg tgatccagct gggcgacgcg
    26761 gccatgtgcg tgaagccggt cgcgttcatc cggcaatgcc tggtcgacgt ccggttcccc
    26821 gaacgcctgt ggaagctcct tcccccactg aaggtcgccg cggccacggg gctcgtcgcc
    26881 ggcatctggg taccgtcgct ggcgttgctc acctgcgcgg ccctggtctg ctacttcgtg
    26941 gtcgcgatcg cggcgcacat ccgcgcccgg gatctcggcc gcaacctgtt cctcaacgcg
    27001 accgcgatgc ttgccctgtg cacggccacg ctggtcttcg tcgtcgaggc cgcctgaccc
    27061 aacacccctc agtcgccgct caggcaccgc cgcctcttgc cccccaggac gaagctgcat
    27121 ctggcccgcc tccttcgccg aagcaggccg accttcccac gacccgtcag gtcagagctt
    27181 cgagggctcc aaatcgacat gcccgttcgc gacgagccag tccagtgctt cacggagggc
    27241 ttccaacgcg gtgtaccgag gggcgaaacc cagcagcgtg cgtgcgcgct cgatgctggc
    27301 gctgatcgag cgagagacgt gctcgcgagt cgttccggcg tgctccgctc cgaccagcga
    27361 cgcgaacgtg ggccagtcga ccagctcgat cacgggctcc cgaccgaacc acccggcgac
    27421 cccgtgcgcg aggccacgca aggtcatcgc ttgctccgcg acgacatgga tgctcgagcc
    27481 gatggccgct gggcgcgtca acgctcgctc gaacgcctgc gcgacgtcgt cggcgtgaac
    27541 gtgatgcagt acgccgagac cgaggtcggg cagcggtagc ggcgcaccca gcgcgagagc
    27601 gcgccacaca gctgggtcga ggttgccggc cggggtgatc acctgccatc cgggaccgct
    27661 gatgtggccg gggtgcagca cgacgctcgg gacaccgccg ccgcgggtct cgcggtgcag
    27721 cagctcctcg atcgccgcct tgccgatgcc gtattcgccc accggcttcc gcggctcgtc
    27781 ctccgtgacc ggcacgcgcg ccacgggacc gtgcacccag accgtgccgc agtgcagcag
    27841 caacggccgg gtgggccgca acgcctcgac gagctgggct gccgactcgg cggtgaaaca
    27901 caccaggtcc accacgacgt cgggatccaa ggacgcgacc cgcgacccga acgtcccggc
    27961 ggcatcctct gcctcccggt cgacggtcac ccgctcgacc cgcgaccact ccggtgcgtg
    28021 gtggtagggc tcacgtgtcc cgcggctgag cgcagcgacc tcatgccccg cgcgcaccag
    28081 tcgggggacc aggtaggagc cgatgtgccc ggtcgctccg acgaccacga cacggctcat
    28141 gcggaacgtc ccgccagaca ggcatagtca gcgtcgcgag gaggtgtcac cgcacgatga
    28201 tgcaccctgc ccaacgccta cggaggccgc cgtgccgatc acgggccggt caggacgtcg
    28261 gggacactca ccacctacga aggcgaccgg cggcgacgaa ccacagcgtc gaccccgcgg
    28321 ggcaatagtg cgagatcggt gtccacgagt cgacaatggt cacgcgagcc cgccgacgta
    28381 caccccttgc gaacgcaggg cctcgacgcc ggtgagcagg gtggaatcgc ttcccgacag
    28441 atcgacgccg gccagcgcgg gcatgtcctg gagcggacgg acgtcggtca gggggttgcc
    28501 gccgatccac agctcgtcga cggcagggca cccggccatt gtggccgcgt cgctgatccg
    28561 gttgccggac acgtccaagg tggtcagggc cggcagcccc tgaagcacgc tcgggtcggc
    28621 aacgatgttg cgcgccagca gcagcctgtc cagccgggac aacgcggcga gcggagtgac
    28681 gtcggcgatg tggttgtcgc tgacgtcgag ctcggtgagg ccggccagtg gggtcagcgg
    28741 cgcgaggccg gtgactgcat tccgcgacaa cccgaggaac cggagcccgg tcagccccga
    28801 gagtggggca aggtccgtca cctcgttgcc ggacaggccc agttcctgga gcgtggccag
    28861 cccctccaac ggagtcaggt cgcgaacccc gttgttcgtc agtgtcaggg tggtcagctg
    28921 cggtacctgc gccaggccgg acaggtcgac gacgtcgttg cccgacaggt ccaggtccac
    28981 caggttcggc aggtggtcga gcccgcccag agccgctatc cgcccatcct gtgcgaggtc
    29041 gccgttgcac ttgaggctga agacgctgtt cagctcccgc tccgacaccc gctcgccgtc
    29101 cgtcgcgccg accgctgacc gcacgcaggc tgccagcgcc gggtcgggga aaagtgccgc
    29161 gagctccagc cggttgccca gctgcgagta ccccgcccag acggtaccca ctgccaccaa
    29221 gacgatcgcc cccaggacag tccaccgagc cgcccaccgg ggaccggtgg agggcaccgt
    29281 gggcttgtcg accccggcac tgcccgtgcg ggccgtgagg gaccaccacc gggtgacgac
    29341 gaccaccagc agcattgcgg ccacgcccac gatgatcgca gccgcgatcg gggtgctctg
    29401 gatgctcccg ccgcggtagg ccaggctctg gacggcgaga ccgagtagcg cgagcgcgta
    29461 cagcagggca gtgacgacca tgatcgccgc ggcgatcacg ccgagcaacc ggtccccgaa
    29521 cagagcagcc agcccggcca ccaccaggac gatgcccacc accaccagcc ccacgggagc
    29581 cagcacggtt gccgcgttcg tgaccacctg aggcaagggg aagttcaggc ggaacagatg
    29641 catcgcgtcg gtgctgaccg tgatcgcaat gcctacgccg gcgacgccga agcctgtgac
    29701 cacaatcgct gcaccgcccg cccgggttct cccgattgac gcagcgaggg tggccatgag
    29761 cggagagcag gccacggcga ccaccagagc gctgctcgac aggccgtgcg gtgccagcac
    29821 cacgcctacc acgatcccgg cgatggcgac gacccatgag gcgacgagca cgctgcgcag
    29881 aacccagcac ccccagacat cggacctcgc ccggccgccg ctgggtgagc acagaactct
    29941 ggccgacaga tcgccatcag tcaacgacgg ccgaacaccg cgagccgccg ctcgctcgcg
    30001 gagagcaaat cgccccgaat ccgcgcgaac cgcacggggt ggaaatgtcc atgggcggct
    30061 ccccggacga aagggaccgc ccatcgtgcc gtcgggacgc aggcgtccgt gacggctcac
    30121 cgactggcga gcggcgatct gtcgacccgc atggcgacga ccggagccgc cgagatcgga
    30181 gtgctcgaga agtctgtcaa caccatggcc agctggctgc agggcagcca atcggaactc
    30241 gccaatctgc ttgaagagca ggccgccctg cgccgggtcg ccaccctcgt cgccgagggc
    30301 accccgccgg agaaggtctt ccccgcggtc aacgaggagg tggcccggct gctcgaggtc
    30361 gcacgcgctt gatgcggatc cctggtgcac agcagcagca ggccggcgcg catcgaggac
    30421 tacgaaggcg ctcccggatc tgtcgccgcg atcgcccgcg agcacggcat ccgttcgacc
    30481 gtctcactcg tgctcgaggt gcgcaacacg caggcaaccg tcccccgaca cacaactaca
    30541 gctgagaatt gagctggctg aattcgccga cggcctgatc gccgtgctgg acgacctgcg
    30601 ggagatctcc cgcggcattc accctgcgtt gcttgccagc ggcggcctgg gtccagcgct
    30661 caaggcgctg gcccgccgcc cgccgctccc ggtcgaaccg ccaaacacgc ccgcgcctca
    30721 cacgcccacg tcaccgtcga acaacgcgac gccctcctga acctctcgat tccgcgacga
    30781 cggcatctgc ggcgccgatc ccgccggagg ctccggcctg acggcctgcg accaggtgca
    30841 ggtcctgagc gggtcgatcg aggtcaacag ccgccccggg gtcgggggcg gcgatcgtcg
    30901 tcgaacctcc actgcagccc gaccaaccgg tcgaccctgc gggtgcccga tatccggccc
    30961 accgaccgtg acctgcagtt tcgccacgac gccatcgccc gcagcgcgcg atcgctgagg
    31021 ccgctccgct gcatgccctt ccagagcgcc gccagaccgg taccagccac gtccaccgac
    31081 acgaccggct cggcggtctt cgccatgaat atcagcaggt cgcatgacat gagccgggtt
    31141 tcggggcacc tacagggcac tcagcggtgc cggagtgtcc ggtagatgag ccggccgacc
    31201 accaagtaga cgatcgccgc cagtccatag ttgacgataa ggccgatctt ggcatcgctc
    31261 ggagtaaaga catctccgaa gccgaacacg aaaatcttcg ccgcaccgta gacgaaagcg
    31321 acggtgccgc tgttctgatt ggcgccgaac aagctgaaca cgatgtgcag cacgaaaacg
    31381 agtgcgatga gagccgtgac cacatggatc atgttagcga tgatattgac ccggtcggcg
    31441 cgacgatcgc gctcacgctg gtacgcgaga ggagattcct cgggctgccg atacgcctgg
    31501 ctggggtaat cacgagagct tggcgacggt tcatacggct cagccggtgg ctgataccgc
    31561 tcgtgctggt acgggtcggg ctcctgctca tatggcgtgg gagcgggtgg tgctggctgc
    31621 tcgtagtgcc ttgtgggctg gcgactcggc tggcgaatcg cggtcgtcgg atgttcggcg
    31681 agaccgggcg gttgctcata tccaccaggg ccttgcggcc gtggtggata gccaccgaac
    31741 gcctgcggag gctgctggta cgcgccggga tttggcggcg gttgcgggcg acgcccgggt
    31801 gcctgcggtg tccgttggtt cggaccctgg tcgtgcccgc cgggatgtgg ttgatccggt
    31861 cggcgagttg tcgcgggacc acggcgaggc ggcggcccct gcggaccttg atactggtcg
    31921 taactcatgc ctcggcattg tgacgatacc gtccggcgaa ggcatacccc ccgggggcaa
    31981 cgacacaccg ctggtgggtc caggaatctg cccgtcggtc cggtccgccc ctggtgctcg
    32041 cggccgcagg gcgagaacgg tactcgttgt ccatccagct gcggccgcgt ctcggcccct
    32101 gaccactcac tacaccgatc tgctcggcac acggtcgctg ttcggtagcg acatccaggc
    32161 gcctcacgtg tccaagtcgc gcccgtcggg tttcgcagag tcagggacct gtgcacgcgt
    32221 atccatgaca gcggtcatgg gtcgccttgg gcacggcagg ccaagatcgg gctcatgacc
    32281 gcgtcgacga ccgttacgcc tccgcccgag ctgcggcgcg tgaccacgct cctcctggtg
    32341 aacctcgcac tgagcgccgt gctcgcggtg ctgttcgcgg cgttccacga caccctgctc
    32401 gactaccagg tcgcccggct ggccctcccc gcctcggcgg acagcgccgc cgtccgcgcg
    32461 agcctcagcg ccggcctgtg gtcccgcgca ttggccgtca tcatcgtcgg cgttgtgtac
    32521 gtcttcctca ttcgccggct gcgccggggc gtgcggcgcg cctacatccg cgtgctggtg
    32581 atctcggtcg tcagcctgct cggcatcgtc tacctggcca cctccggcca gtacccgtcg
    32641 tgggtgctcg tcgagcaggt catccaggct ctcgttctgc tgaccctgct atgggcggtg
    32701 acgcgcccgg ccgtgcgccg gtacttctcc cggagcgccc ggcccgcatg acggcggcgc
    32761 cgcgcgtgga cactgatccg cgtttcgggc accccacacc cacagcatga ccctggccag
    32821 ctacgacctc acctggctgc gccgcatcgg attgatcatc caaatcgacg gccgcatccg
    32881 ctatcggctc accgacgacg gcctcgcctt cgcgatcttc tacagcaagt tgcactaccg
    32941 ggtcctgtga ccactgctgg ccaccacgca ccgcaagccc taccaccgat ccgcgccgcg
    33001 ctgcgctgcg gactaacggc caacacctcg acgcccgcct cgccaacgct cgattaccgg
    33061 tggctgctgc ctgaaaccct gaccaagtgc cgaagttcga acgaccaaat cgctagaggt
    33121 tgatcacctc gaaggacacg gcaaaacggc agcccagtcg cgtcccagcc gtgcgggcca
    33181 tcgcgtccag gaactcgcgt ggctcgctca tggcgttggg cccgagattg cgcaggtact
    33241 caccgtgtgc ctccagcgag gcaacgccac ggtcgaaggt gtcggtcaca ttgaccccgt
    33301 gcgctgccag cgggctgtcc gccaccagga tttgcgtcac ccgccacggc tgcagtcctt
    33361 ccgccagcaa ctcgcggaag acccagcggt taccggcgtc ccgcacgccg tcgatcacgg
    33421 cgcgtcccag ggcgatgtgg tctgcctggt tctgcactcc cggttcccac gtgtcgtggt
    33481 ggttgccggt cacgatgatg tccggccggt gccggcgtac ggcgcgggcg acggcgccgc
    33541 gcaggtcgag gccgtattcc agcattccgt cgggataacc gaggaactcg acgacatcca
    33601 cgccgaccag cgatgcgcct gcgcgttcct cggcctcccg cagagggccg cactgctcgg
    33661 gttcgatggt ctcgatgccg gcttcgccgc tggtcaccag acagtaggcc accttcttgc
    33721 cctgatcggt ccaacgggcg atcgccgccg ccgtgccgta ctcaaggtcg tccggatgcg
    33781 cgaccaacgc cagcgcactg gaccacgact cttcgaccgg cagcaacggc tcaccaggct
    33841 gacacacgtc acgacagtag cctggcaatc ctttatcgac gtgctactga cgtaccagac
    33901 gcggcgcagg cttggcgtca aatgcggggt cgaaactgag gaaccggcat acccgacatc
    33961 accaagtggc ggcgacggac agtgccaagg ttaacagagt ctaacggtag gcattttcgt
    34021 gaccactctt cgggag
//
LOCUS       DASRSC010000151        15473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3489198,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000151 DASRSC010000000
VERSION     DASRSC010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 15473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15473
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3489198"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            190..891
                     /locus_tag="VFE39_07385"
     CDS             190..891
                     /locus_tag="VFE39_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6258457.1"
                     /translation="MATRGTTLPARRADPVRRGGVVVVSDSSGAPDWVVDWCRHSGRW
                     LRRQHLPVAAEGDLPPSAQPVGEIAEIAAAAARRGGAVLAVSGPAPVRSGPRRVVAAV
                     QALPDDAPVLVDAAAVALHMGAELVVTHGVPTSFAERSVGLDAAVDNARRLLEAAVGA
                     AVDAVPGLTVEPRLLRVRPHEMVGEGLDADLLVLGGPRLPAPGGLGLVACSALHHAPC
                     PVLLVPRVGRADFDF"
     gene            972..1157
                     /locus_tag="VFE39_07390"
     CDS             972..1157
                     /locus_tag="VFE39_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HET6258458.1"
                     /translation="MRCSNGDQIVIETTTLDTPPRRGVVVEVIGDGEREHYRVRWQDG
                     HESVYFPGPDARVVPQG"
     gene            complement(1165..2613)
                     /locus_tag="VFE39_07395"
     CDS             complement(1165..2613)
                     /locus_tag="VFE39_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6258459.1"
                     /translation="MAQRPISGVGLRRELSVWEAIGLSLALMAPSMAANINPQGTAGT
                     VGRAVPLAFALATVGVLLIAYTFVRLSQRFHHAGSVYGFVGATLGPRAGVVAGWSLLG
                     TYVFYGVVTSTAAGIFGAAALDSLGIWTDQPPWAGFAVGAVALLAVLALAVAPVRGGT
                     RVLLSIEVATVALILVVAVVILVRLATGTAPGGRTIDLSVFTVAPGTEVSAVFLGVVF
                     GFLSFAGFEAAATLGEEAREPRRSIPRAILGTAIFGGVYFVFVTAVEVMGFGTDDAGL
                     AAFTSSSSLLGDLGGQYVAAWVGDLITVGAAISAFGCALACAVGAARMLFALSRDGVG
                     PAVLAEVSPTRATPTRATIAVVAAMYVIIALTWSVLGGEPFELFVASGTIGTLILLVV
                     YALATIGAARLLFFSGRREVAAWEVVVPALALVLIGYTLFRNVAPYPEGAAGVYPMVS
                     AAWILLGVVAVLVRPGAARRAGRQLTANEGLA"
     gene            complement(3039..3290)
                     /locus_tag="VFE39_07400"
     CDS             complement(3039..3290)
                     /locus_tag="VFE39_07400"
                     /inference="COORDINATES: protein motif:HMM:NF021464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2180 family protein"
                     /protein_id="HET6258460.1"
                     /translation="MHCLDCMTAASDAPFGAEDVVALCAYCGAAVCYHHAHTTRVDPP
                     RIGLVSQARTGARRILCSECAGVTPTASFPRPRVGAAMR"
     gene            3423..4148
                     /locus_tag="VFE39_07405"
     CDS             3423..4148
                     /locus_tag="VFE39_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6258461.1"
                     /translation="MEAEAGMAAGVTAKARGGAKDRALDYVKSRILSGAFRGGELISE
                     GEVARALEMSRTPVREAFLRLEAEGLLRLYPQRGALVVPVSPEEIRAVIEARLVLEQF
                     AAQKVIRRDAGMRGETADALADELVRQRRAGDAADLPEFLDADRLFHATLLDSAQNAI
                     LGAVYASLRDRQVRMIGESALRDPRRVDTILAEHERIAAAVRNGDLEEALEAIRVHLT
                     GTARALGLALDTGPAFGTRSDAR"
     gene            complement(4363..5544)
                     /locus_tag="VFE39_07410"
     CDS             complement(4363..5544)
                     /locus_tag="VFE39_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HET6258462.1"
                     /translation="MSPSPELIAVTVVLFVAIAAYALFAGADFGGGIWDLLAGSTRRG
                     AVPRAAIDASVTPVWEGNHVWSIFVLVILWTAFPPAFAAIMTALFAPIALSLLGIFLR
                     GLGFAFRHEAERLHMQRLNGALFAASSLMAPFFLGTVIGAVATGAVPAAPSGQVLSAW
                     TSPTALVTGGLFVATCAYIGAIYLVADSEQRKQPEMMRYFSYRAIAAGVVSGVLAAVN
                     LLLMRASSPYTFDRLVGPALPLVVVSVVAGIAALVLVVLRRMHLMRIAAGLAVAAVVA
                     GWGWAQYPWLLPGSLTLQAGSAPSASLWAELAVAGLAVVFVLPPFAWLYWLQQHGRLQ
                     ESEISESLRLARATTSEPVVPASPAPVHHEHRLVTTVLIASLATELVRQVLERRRSRR
                     G"
     gene            complement(5541..6935)
                     /locus_tag="VFE39_07415"
     CDS             complement(5541..6935)
                     /locus_tag="VFE39_07415"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HET6258463.1"
                     /translation="MTSYALVLADGTVPARSQMGASLGFHIIFACFGIAFPAVVLIAH
                     WLGLRRGDAVALLLARRWSKVMAVIFAVGAVSGTVLSYELGLLWPGLMGRFGAAFGIP
                     FSIEGIWFFVEAIFTGIYLYGWRGLSPWLHWWSGVPIAVTGLLGAWAVVAANSWMNQP
                     GGFTLTDGRITSVDPWAVIFNSASSYEVPHMILAAYMVASLLTAGVYAVGMLRGRWDR
                     YHRLGFLIPFAVGAVAAPIQVFVGDIAARMIASQQPQKFAAMELVDRTGTNVTEWLGG
                     IYWNGQVYFGVGIPDLDSILVGFSPNTTVIGWDAAPPELRPPLPNLIHLAFTGMVGIG
                     TALVVLGAWQGWVWWFHRRVPRTRWFLVPAALSGVGAVLAMELGWIVTEVGRQPWTVY
                     GVLLTRDAVTPAGGVIITLAVTLVIYLVLTGVCIVVPWLMGRRWRAEGDPRLVEDQAT
                     PYGPPVERVREVRS"
     gene            7044..7226
                     /locus_tag="VFE39_07420"
     CDS             7044..7226
                     /locus_tag="VFE39_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258464.1"
                     /translation="MIAAATVFWVGVGIGALGLVEDDLYVVGLLIMLIGMIGVLVRVT
                     DWFSAGASGLGRRAPR"
     gene            complement(7693..8754)
                     /gene="nrfD"
                     /locus_tag="VFE39_07425"
     CDS             complement(7693..8754)
                     /gene="nrfD"
                     /locus_tag="VFE39_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET6258465.1"
                     /translation="MTRDRSTASLTDTTREQRMVPEAEFRSYYGRPVLKPPVWEWMVP
                     AYLFTGGLAAGSAVLAAGADVTGRPSLRRAGRLGSLVALTASGVLLVGDLGRPERFHH
                     MLRVAKPTSPMSVGTWIITAFSPGAGIAAIDELVPRRWRRTLLGRLLHRLARPAGMSA
                     ALVAPGLASYTAVLLSHTAVPAWKEAGEVLPFVFTGSAAAASGGLGMMFTPVDEAAPA
                     RWFAICGATMELTASRSMETRLGLIAEAYTTGRAHRLRRGSELLTVAGLVGTLLGRRS
                     RTVSGAAGLALAAGSALQRFGVFEAGVVSAKDPKYTVVPQRERLERGLRASASVPGDE
                     AVVGVDPDEPISRARSGGP"
     gene            complement(8751..9788)
                     /locus_tag="VFE39_07430"
     CDS             complement(8751..9788)
                     /locus_tag="VFE39_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET6258466.1"
                     /translation="MKHTKSLPKPSADVAGDAGHVDHPPRVGFFTDTSVCIGCKACEV
                     ACKEWNLVPDDGFDLLGMSMDNTGSLGASTWRHVAFVEQPVPLGAQTSGLAGLPTGTS
                     AGTAAAEVVASRLRAAERGGGELGTSPLEVPEVPEDLGMPTFQRPGDGTGAESRTDFR
                     WLMSSDVCKHCTHAGCLDVCPTGALFRTEFGTVVVQQDICNGCGYCVSGCPYGVIDRG
                     VVDGRAHKCTLCYDRLHDGLEPACAKACPTDSIQFGELDELRERAANRVDQLHERGVT
                     EARLYGADPDDGVGGTGAFFLLLDDPEVYGLPPDPVVPTRDAPAMWKAAGAAGAAFLT
                     AGVAAFLGTRR"
     gene            complement(9785..13078)
                     /gene="fdh"
                     /locus_tag="VFE39_07435"
     CDS             complement(9785..13078)
                     /gene="fdh"
                     /locus_tag="VFE39_07435"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(12509..12511),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HET6258467.1"
                     /translation="MGVPRWLGSWPIYRQLTGPDRLGRGAAAESPLSAAMQPRTATAD
                     RVVKSVCPYCAVGCAQDVFVFDEKVIQIEGNPDSPVSRGRLCPKGSASKQLVTGPQRE
                     QKVLYRAPYATDWIELELDTAMDMVVDRVLDARAKGWQDHDDQGNQLRRTMGIAHLGG
                     ATLDNEENYLIKKLYTALGAIQIENQARIUHSATVPGLGASFGRGGATNYQQDLVNSD
                     CIVIMGSNMAEAHPVGFQWVMEAKARGTRVFHVDPRFSRTSAVANVHVPLRAGSDLAF
                     LGGVINYILSNELDFREYVVAYTNAAHLIREDFRDTEDLDGLFSGYDPETATYDPETW
                     HYEGADPTGSSPGQEAPAEAKQHARGDTHGAAGPTLDGVLGKMASDPTLQHPRCVYQV
                     LKRHFARYTPEMVEEVCGTPREQFLELCEAWTANSGRERTSALVYSVGWTQHSVGSQY
                     IRAGSIIQLLLGNIGRPGGGILALRGHASIQGSTDVPTLFNLLPGYLAMPSSVSHPDL
                     ESYLESICGGENQKGFWCNANAYMVSLLKEYWGKRATPENDFCYDYLPRISGDHGTYR
                     TLMDMIDGKVSGYFLIGQNPAVGSAHGRLQRLGMANLDWVVVRDLAMIESASFWKDAP
                     EIETGEIVTEKCRTEVFFFPAASHVEKEGSFTQTQRMLQWRDKAVEPTGDQRSDLWFY
                     YHLGRRIRERLAESSDDRDRPILDLAWDYEVHGDEPSAEDVLKHISGRDLTTGRLVDG
                     YMDLKADGTTSCGCWIYSGVYAGGVNKAARRTSRLDQDETALEWGWAWPYNRRVLYNR
                     ASADPQGRPWSERKALIWWDPDRGEWTGHDVPDFEQKKPPDYVPPPGSSGVQALSGDD
                     PFIMQADGKGWLFAPRGLVDGPIPTHYEPHESPVRNPLYPQQGNPTRMVYGRRDNPSN
                     PSPPEAHSDVFPYVLTASRLTEHHTAGAMSRALPYLAELQPALTVEVSPELAAERGLT
                     HLGWAHVITSRAAIEARVMVTDRMAPLRVQGRTLHQIWLPFHWGTVGLVTGDAFNDLL
                     GLFLDSNVFIQESKVATCDIQPGRRPRGHELLDYIRGYRERAGITTDTGTHVATGSGV
                     AHVEEELPS"
     gene            13261..13785
                     /locus_tag="VFE39_07440"
     CDS             13261..13785
                     /locus_tag="VFE39_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET6258468.1"
                     /translation="METWDAIRSRRNVRVYENRPIPREDLERILEAGRRAPSSQNWQP
                     WDFVVVTDREQLRQLAGVWQGARHVAGSAATIALVAPQPEDARQRDWIHYDFGQATMN
                     MMVTAADLGIGSGHSAVADQDLARRLLGNPDSTWCQSLIALGYPADRPLTPIQRPNRR
                     PFDDVVHWGRWSRG"
     gene            14077..14877
                     /locus_tag="VFE39_07445"
     CDS             14077..14877
                     /locus_tag="VFE39_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6258469.1"
                     /translation="MERRRLGVEGMEVPVIGLGTWRRLEAAATGGGHVAVVTRALDAG
                     VRFFDSSPMYGRAEELLAEALGDRRGEAVVATKIWTASPVEGRRQLDRALRLYGGHVE
                     LMQIHNLVAWREHLPLLLAAREEGRIDRIGATHYAVVRELETVMRTGGIDAVQIPYNP
                     RQREAEQRILPLAADLGLGVVVMRPFGEGELLDLDIGEAQLAPLYPFGVRTWAQALLK
                     WGLSDPRCTVAIPATSRPPRIDENVAAGDWPWFGPDERELVHRLALGT"
BASE COUNT         2407 a         5435 c         5455 g         2176 t
ORIGIN      
        1 ctgccgcacc gaggagcctc cggaccggcc gccgagccgg ttgtgacgaa ctccctcacc
       61 gtcgggtagc gcgttgccct cacagaatcg aacggcccac cgcatgctgc gctccgtcgt
      121 cgagatgcac tcggggttcc caccatcatg tactggaccg ggccgccggc tccgaggaag
      181 gtcgcaggag tggccacccg cggtacgacc ctcccggccc gacgcgccga tcccgtacgt
      241 cgcggcggcg tcgtggtcgt gagcgacagc tcaggcgcgc cggactgggt ggtcgactgg
      301 tgccggcact ccgggcggtg gctacgcagg cagcacctgc cggtggccgc ggagggcgac
      361 ctcccgccct cggcccagcc ggtcggcgag atcgccgaga tcgccgcggc cgcggcgcgc
      421 cggggcgggg ccgtcctcgc cgtgtccggg cccgcgcccg tccggtccgg cccacgccgg
      481 gtcgttgcgg ccgtgcaggc cctgcccgac gacgcaccgg tactggtcga cgctgcggcc
      541 gtcgccctgc acatgggggc cgaactggtc gtaacccacg gcgtgcccac ctccttcgcc
      601 gagcgcagcg tcgggctcga cgctgcggtg gacaacgccc gccgtctgct cgaggcggcc
      661 gtcggagcgg ccgtcgacgc ggttccgggc ctcaccgtcg agccgcgcct gctccgcgtc
      721 cgcccacacg agatggtcgg tgaggggctc gacgcagacc tcctcgtcct cggcggcccg
      781 cgtctgccgg cgccgggcgg gctcggtctc gtcgcctgca gcgccctgca ccacgcgccg
      841 tgcccggtgc tgctcgttcc acgagtcggc cgggcagatt tcgacttctg aggctctgga
      901 cgtcctggcg gtcttgtccg cgcccggctg cggttccaaa ctggtcacgc gaaagtggag
      961 gaggacgagc gatgcggtgc agcaacggag accagatcgt catcgagacc accacgctgg
     1021 acacaccgcc tcggcgcggc gtggtggtcg aggtgatcgg cgacggcgag cgggagcact
     1081 accgcgtccg ctggcaggac ggtcacgagt cggtctactt ccccggcccg gacgcaaggg
     1141 tggttccgca gggctgaacg ccgctcaggc cagcccctcg ttcgccgtga gctgccggcc
     1201 ggcccggcgg gcggctcccg gccggaccag caccgcgacg acgccgagca ggatccaggc
     1261 ggccgacacc atcgggtaga caccggccgc gccctccggg tagggcgcga cgttgcggaa
     1321 cagcgtgtac ccgatcaaca ccagcgcgag cgccgggacg accacctccc acgcggccac
     1381 ctcccgccgt ccggagaaga acagcaggcg ggcggcgccg atcgtggcca gcgcgtacac
     1441 gaccagcagg atcagggtgc cgatcgtccc ggaggccacg aacagctcga acggttcccc
     1501 acccagtacc gaccatgtca gggcgatgat cacgtacatc gcggcgacga cggcgatcgt
     1561 cgcccgcgtc ggggtggcgc gcgtcgggga gacctccgcg agcaccgccg ggcccacgcc
     1621 gtcgcgggag agcgcgaaca gcatgcgcgc tgcacctacc gcgcaggcca gcgcgcagcc
     1681 gaacgcgctg atcgcggcac cgaccgtgat gagatccccc acccaagccg ccacgtactg
     1741 cccgcccagg tcgcccagga gcgaggacga cgacgtgaag gcggcgagcc ccgcatcgtc
     1801 ggtgccgaaa cccatcacct ccaccgcggt gacgaacacg aagtagacgc caccgaagat
     1861 cgcggtgccc aggatcgctc gcgggatgct ccggcgtggt tcgcgagcct cctcacccag
     1921 ggtcgcggcg gcctcgaaac ccgcgaagga caggaacccg aagacgacgc cgaggaacac
     1981 ggcggagacc tcggtgccgg gcgccaccgt gaacaccgag aggtcgatgg tccggccgcc
     2041 gggggccgtc ccggtggcca accgcacaag gatgaccacg gcgacgacga ggatcagcgc
     2101 gacggtggcc acctcgatcg acagcagcac ccgcgtaccg ccgcgtaccg gtgcgacggc
     2161 gagcgccagc accgcgagca gggccacggc gccgacggcg aaccccgccc acggcggttg
     2221 gtccgtccag atcccgaggc tgtccagggc ggcagcgccg aagattcccg ccgctgtgga
     2281 ggtgacgacg ccgtagaaca cgtaggtgcc cagcagcgac cacccggcga ccacgccggc
     2341 ccgaggaccc agcgtcgccc cgacgaagcc gtagacgctg ccggcgtggt ggaagcgctg
     2401 gctcagccgg acgaaggtgt aggcgatgag cagcaccccg acggtcgcca gcgcgaaggc
     2461 gagcggcacg gcgcgcccga ccgtgcccgc cgtcccctgc gggttgatgt tggccgccat
     2521 ggacggcgcc atcagcgcca gcgagagacc gatcgcctcc cacacgctca gctcgcgacg
     2581 gagtccgaca ccggagatcg gcctctgcgc cacggcgcct ccctcagctg atcgtcagat
     2641 cagcagatgg tcggccagaa cctcgcaaaa gtcactcccc acccgcagcc gtcgcccatc
     2701 tgggcgcatt tgccccgcag ccgctgatca agacacaaaa gcgccccgca aagatcgaca
     2761 aacgaccccc gcgccttgat caccgcgctg ggtggcgagg ccctgtcggc tgctgctgcg
     2821 catatgtgct cccccgccct gcgggtcgac cgggctccgc ctcgcgaggc gcttcggtga
     2881 gattgccctc gatcagcagt gcgggagcgg gcctcgatgc ccaacccccg gtcgctgcac
     2941 cggcgcatgt cgttcccgcg agccgggcga ggttgtcggt ggcgtgcccg cgatccgtcg
     3001 ggatcgtccg cggtcgacat gacgcccgcg atcggtggtc atcgcatggc ggcgccgacg
     3061 cgcgggcgcg ggaagctggc agtgggggta acacccgcgc actccgagca caggatccgc
     3121 cgggcgccgg tccttgcctg ggagacgagg ccgatccgcg gcgggtcgac ccgggtggtg
     3181 tgggcgtggt ggtagcagac ggcggcgccg cagtaggcgc agagcgcgac cacgtcctcc
     3241 gcgccgaacg gcgcgtcgct ggcggcggtc atgcagtcga gacaatgcat tggatctacg
     3301 tcctcccctg ttgaacgcaa ctagtatcta agttgcatct ccgacggtac ggcggccggg
     3361 gcatgaccgc cacccgcccg gggatcggtg tacgttacgt ggaccacttg tatgccactg
     3421 ggatggaagc ggaggcgggc atggcggccg gggtcactgc gaaagccagg ggcggggcga
     3481 aggaccgtgc gctggactac gtcaagagcc ggatactgag cggcgcattc cgcgggggtg
     3541 agctgatcag cgagggtgag gtcgccaggg ccctggagat gtcacggacc ccggtccgcg
     3601 aggcgttcct ccggctcgag gccgaggggc tgctgcgcct ctacccgcag cggggagctc
     3661 tcgtggttcc cgtatcgccg gaagagatcc gggccgtgat cgaggcgagg ctggtcctgg
     3721 agcagttcgc tgcgcagaag gtgatccgcc gcgacgcggg tatgcggggg gaaaccgcgg
     3781 acgcgctggc cgatgagctg gtgcggcagc ggcgggccgg tgacgccgcc gaccttcccg
     3841 agttcctcga cgccgaccgg ctcttccacg cgacgctgct cgacagcgcg cagaacgcca
     3901 tcctgggcgc ggtctacgcg tcactccggg accggcaggt gcgcatgatc ggcgagtccg
     3961 cactgcgcga tccccggcgg gtcgacacga tccttgccga gcacgagcgc atcgccgctg
     4021 cggtccgcaa cggtgacctc gaggaggcac tcgaggcgat tcgggtgcat ctgacgggca
     4081 ccgcccgggc gctgggcctc gcgctcgaca cgggtcctgc gttcgggacg cgatcagacg
     4141 ctcgctgacc ggacctgtgc ccgcacgaag cgccctttgc gtcacataaa cgacgacggc
     4201 acctccagcg ccgatcgcgt cgtcaccacc accaacagac ccaccaccag caccgcggct
     4261 gtcaccgtcc gccgtgccaa ctgcccgaaa cgcgttgcgc agacaatgcc agctgtcggg
     4321 ccgacccacg gtgcagccgg ttgggcgcct ccgggggcac cctcacccgc ggcggctccg
     4381 gcggcgctcc aggacctgcc ggacgagctc ggtcgccagc gatgcgatga gaacggtcgt
     4441 cacgagcctg tgctcatggt gcacgggagc ggggctcgcc ggcaccaccg gctcgctggt
     4501 ggtggccctg gccagacgga gactctcgga tatctcgctt tcctgcagcc gtccgtgctg
     4561 ctgcagccag tagagccagg cgaacggggg cagcacgaac accacggcca ggccagcgac
     4621 ggccagctcg gcccacaggc tcgccgacgg tgcgctgccg gcctgaaggg tcagcgagcc
     4681 gggcagcaac cacgggtact gggcccatcc ccagcccgcc accaccgcgg ccaccgcgag
     4741 gccggcggcg atccgcatca ggtgcatgcg ccgcaggacg accaggacga gcgcggcgat
     4801 ccccgccacc accgacacga cgacgagcgg gagcgccggg ccgacgagcc ggtcgaaggt
     4861 gtagggcgag ctcgcgcgca tgagtaggag gttgaccgcc gccagcacgc cgctgaccac
     4921 gccggcggcg atcgcccggt acgagaagta gcgcatcatc tcgggctgct tgcgctgttc
     4981 ggagtcggcc acgaggtaga tcgccccgat gtaggcgcac gtggcgacga agagccctcc
     5041 ggtgacgagc gctgtggggc tggtccacgc gctgaggacc tgcccggacg gcgccgccgg
     5101 caccgctccc gtcgccaccg ccccgatgac cgtcccgagg aagaacggcg ccatcagcga
     5161 ggatgcggcg aacagtgctc cgttgagccg ctgcatgtgc agccgctcgg cctcgtgccg
     5221 gaaggcgaag ccgaggccgc ggaggaagat cccgagcagg gacagggcga tcggggcgaa
     5281 cagcgccgtc atgatggcgg cgaaggcggg cggaaaggcg gtccacaaga tcacgagcac
     5341 gaagatgctc cagacgtggt tgccctccca taccggcgtc acggaggcat cgatggcagc
     5401 gcgcggcacc gccccgcgtc tggtactgcc ggccagcagg tcccagatcc caccgccgaa
     5461 gtcggccccg gcgaacaggg cgtaggccgc gatggccacg aagagcacga ccgtgaccgc
     5521 gatcagctcc ggtgacgggc tcatgaccgg acctcccgaa cccgttcgac cggcggaccg
     5581 tagggcgtgg cctggtcctc tacgagccgg ggatccccct ccgctcgcca ccgacggccc
     5641 atcaaccagg gcaccacgat gcacacgccc gtgagcacca ggtaaatcac gagagtcacg
     5701 gccagggtga tgatcacccc gcccgcaggg gtgaccgcat cgcgggtcag cagtacgccg
     5761 tagacggtcc agggttgccg gccgacctcg gtgacgatcc agccgagctc catcgccagc
     5821 accgccccga ccccggacaa ggcagcggga accaggaacc agcgcgtccg gggaactcgc
     5881 cggtggaacc accagaccca cccctgccat gcccccagca cgaccagcgc cgtcccaatg
     5941 cccaccatcc cggtgaaggc cagatggatc aggttgggca acgggggccg cagctcgggc
     6001 ggggcggcgt cccagccgat caccgtggtg ttcggggaga agccgacgag tatggagtcc
     6061 aggtcgggga tcccgacgcc gaagtagacc tgcccgttcc agtagatccc gcccagccac
     6121 tcggtgacgt tggtcccggt gcggtcgacc agctccatcg ccgcgaactt ctgcggttgc
     6181 tgggaggcga tcatccgggc cgcgatgtca ccgacaaaga cctggatggg cgccgcgacc
     6241 gcgcccaccg cgaagggaat caggaaaccg agccggtggt agcgatccca ccggccacgc
     6301 agcatcccca ccgcgtacac gcccgccgtc agtaagctgg cgaccatgta cgcggccagg
     6361 atcatgtgcg gcacctcgta gctggaggcg ctgttgaaga tcaccgccca cgggtcgacg
     6421 gaggtgatcc gcccgtcggt caacgtgaac ccgccgggct ggttcatcca cgagttcgcg
     6481 gccaccaccg cccacgcccc gaggagcccg gtcaccgcta tcggcacccc gctccaccag
     6541 tgcagccagg gcgagagccc ccgccagccg tagaggtaga tcccggtgaa gatggcctcg
     6601 acgaagaacc agatcccttc gatgctgaac gggatcccga aggcggcacc gaagcgcccc
     6661 atcaagccgg gccacagcag ccccagctcg taggacagca cggtgcccga caccgcgccc
     6721 accgcgaaga tcacggccat caccttcgac caccgccgcg ccagcagcag ggccaccgcg
     6781 tcgcccctcc ggagccccag ccagtgggcg atcaggacga cggccgggaa ggcgatcccg
     6841 aagcatgcga agatgatgtg gaagccgagg gacgccccca tctggctgcg cgccggaacg
     6901 gtgccgtcgg ccagcacgag ggcgtaggag gtcacgacag cccccctggg atcggtgcga
     6961 cgcgccgtgc gacctccgcg ggcctgtcgg cgcgcatact acgagtgggc gcagacagcg
     7021 cggaggccgg aggtacggat ctgatgatcg cggcggcgac ggtgttctgg gtgggtgtcg
     7081 gcatcggggc cctcggtctc gtcgaagacg atttgtacgt ggtcggtctc ttgatcatgt
     7141 tgatcggcat gatcggcgtg ctggtacgag tcaccgactg gttcagcgcc ggtgcatccg
     7201 gcctcggcag acgcgctccg agataggccg attgcaggtg gccgagcgcg tggacgtcgc
     7261 gctggatggc taagcgaaac gcccagtccc tgaacagctc gagcccgcag agctgcggcc
     7321 acccgggacg agggtgcttc cttctacgac agcctctcct ccggcacccg gcggcacaac
     7381 accgccagcc ccgtgcgggt ctgggtgctg acccgagtcc ccgcatacct gccaccgggt
     7441 agcaaaagtc accagccgct acgaacacgc ctgttcgaag caaccaggga cgagttcgtc
     7501 gcgacacccg acgcccgtgc ccccgcaccg gcggcgctgc gaccttggcc ggcgaccgtg
     7561 cagaggtcgt ccgcgttccg gaggcaatgc acattcgact gcgcgccagc gcattcacct
     7621 gccgagcctc ggtatcagtt ggtggccggc ccgccggact ggcggcgcgc gggccaggtg
     7681 tcgcccggat cgttacgggc ctcccgatcg cgccctgctg atcggctcgt cgggatcgac
     7741 ccccacgaca gcctcgtcgc ccggcacgga tgcggacgcc cgcaacccac gctcgaggcg
     7801 ctctcgctgg gggacgacgg tgtacttcgg gtctttggcc gataccacgc cggcctcgaa
     7861 cacgccgaac cgttggagcg cgctgcccgc ggccagcgcg agcccggcgg ccccggaaac
     7921 ggtccggctg cgccgtccga gcagcgtgcc gaccagaccg gccacggtga gcagctctga
     7981 cccgcggcgc agccggtgcg cccggcccgt cgtgtaggcc tccgcgatca ggccgagccg
     8041 ggtctccatc gaccgagacg ccgtgagctc catcgtcgcg ccgcagatcg cgaaccatcg
     8101 ggccggggca gcctcgtcca ccggcgtgaa catcatgccg aggccaccgg aggcggccgc
     8161 cgctgatccg gtgaagacga acgggagcac ctctccggcc tccttccacg cgggcacggc
     8221 cgtgtgcgag agcagcacag ccgtgtagga cgcgaggccc ggcgcgacga gcgcggcgga
     8281 catgccggcc gggcgggcga gccggtgcag cagccgcccg agcagggtgc ggcgccagcg
     8341 ccgcggcacc agctcgtcga tcgccgcgat tcccgcacct gggctgaagg cagtgatgat
     8401 ccaggtgccg acgctcatcg gggacgtggg cttggcgacg cggagcatgt ggtggaaccg
     8461 ttcgggccgg ccgaggtcgc cgaccagcaa gacgccgctg gcggtcaacg cgacgagcga
     8521 gccgagccgg ccggcccgcc gcaagctcgg tcggccggtg acgtccgccc cggccgcgag
     8581 caccgcggaa ccggccgcga gcccgccggt gaacaggtag gcgggaacca tccattccca
     8641 caccggaggc ttcagcaccg gcctgccgta gtaggaacgg aactccgcct ccgggaccat
     8701 tcgctgctcg cgggtggtgt cggtgaggga tgcggtggag cggtcccggg tcatcgccgg
     8761 gtcccgagaa acgcagcgac gccggcggtc aggaaggccg ccccggcagc gcctgccgcc
     8821 ttccacatcg cgggagcgtc gcgggtcggg acgaccgggt ccggcgggag accgtagacc
     8881 tcgggatcgt cgaggagcag gaagaacgcg ccggtaccac cgacaccgtc atcggggtcg
     8941 gccccgtaga ggcgggcctc ggtgacaccc cgctcgtgca gctggtcgac ccggttcgct
     9001 gcgcgctcgc gcagctcgtc gagctcgccg aactggatgg agtcggtcgg gcaggccttg
     9061 gcgcacgccg gctccagccc gtcgtggagc cggtcgtaac acaacgtgca cttgtgcgcc
     9121 ctgccgtcga cgacaccccg atcgatcacg ccgtacgggc agccggaaac gcagtagccg
     9181 cagccgttgc agatgtcctg ctggacgacg acggtgccga actcggtacg gaacagcgcg
     9241 ccggtggggc agacgtccaa acagcctgcg tgcgtgcagt gcttgcacac gtcggaggac
     9301 atcagccagc ggaagtccgt gcggctctcc gcgcccgtgc cgtcgcccgg acgctggaac
     9361 gtcggcatgc ccaggtcctc gggcacctcc ggaacctcga gcggcgacgt gccgagttcc
     9421 ccgccgcccc gctcggccgc ccgcagcctg ctggccacga cctcggccgc cgcggtgcct
     9481 gcggacgtcc cggtgggcag cccggccagc ccggaggtct gagccccgag gggaacgggc
     9541 tgctcgacga acgcaacgtg ccgccaggtg ctcgcgccca gcgagcccgt gttgtccatc
     9601 gacattccga gcaggtcgaa cccgtcgtcg ggaacgaggt tccattcctt acaggccacc
     9661 tcacatgcct tgcagccgat gcagacggag gtgtcggtga agaagccgac gcgtggcgga
     9721 tggtcgacat gaccggcgtc gccggcgacg tcggccgacg gcttgggcag gctcttcgtg
     9781 tgcttcacga tggcagctcc tcctcgacgt gcgcgacgcc gctcccggtg gcgacgtgtg
     9841 tgccggtgtc ggtggtgatg ccggcacgct cccggtaccc gcggatgtag tcgagcagct
     9901 catggccgcg tgggcgacga ccgggctgga tgtcgcaggt cgcgaccttg ctctcctgga
     9961 tgaacacgtt ggagtcgagg aacaggccga gcaggtcgtt gaacgcgtct ccggtgacga
    10021 gcccgacggt gccccagtgg aagggcaacc agatctggtg cagggtccgg ccctggacgc
    10081 gcagcggcgc catccggtcg gtgaccatga cccgggcttc gatggcggcg cggctggtga
    10141 tcacgtgggc ccagccgagg tgcgtcagcc cgcgctcggc ggcgagctcg ggggagacct
    10201 cgacggtgag cgcgggctgc agttctgcga ggtagggcag cgctctgctc atcgcgccgg
    10261 cggtgtggtg ctcggtcagc cgggacgcgg tgagcacgta ggggaagacg tcggagtgcg
    10321 cctccggtgg ggacgggttc gaggggttgt cgcggcgccc gtagaccatg cgtgtggggt
    10381 tgccctgctg ggggtagagc gggttgcgca ccggcgactc gtgcggctcg tagtgcgtgg
    10441 gaatcggacc gtcgacgagt ccccggggtg cgaacaacca gcccttgccg tcggcttgca
    10501 tgatgaacgg gtcatctccg ctcagggcct gcacaccgga ggaaccggga ggcggcacgt
    10561 agtcgggcgg ttttttctgc tcgaagtcgg ggacgtcgtg accggtccat tcgccacggt
    10621 ccgggtccca ccagatgagc gccttccgct cactccacgg gcggccctgc ggatcggccg
    10681 aggcgcggtt gtagaggacc cgccggttgt acggccacgc ccacccccac tccagggcgg
    10741 tctcgtcctg gtcgaggcgc gatgtgcgcc gtgcagcctt gttgacgccc ccggcgtaca
    10801 cgccgctgta gatccagcaa ccgcaggacg tcgtgccgtc cgccttgagg tccatatagc
    10861 cgtccaccag ccgaccggtg gtcagatcgc gcccacttat gtgcttgagg acgtcctcag
    10921 cggacggctc gtcgccatgt acctcgtagt cccaggccag gtccaggatc ggccggtccc
    10981 ggtcgtcgct cgactcggcc agccgctccc tgatccgccg gcccaggtgg tagtagaacc
    11041 acagatccga gcgttggtcg ccggtgggct cgacggcctt gtcccgccac tgcagcatcc
    11101 gctgggtctg ggtgaacgaa ccttccttct ccacgtgcga ggcagcaggg aagaagaaca
    11161 cctccgtacg gcatttctcc gtgacgatct cgcccgtctc gatctccggc gcgtccttcc
    11221 agaacgaggc gctctcgatc atggcgaggt cgcggaccac cacccagtcc aggttggcca
    11281 ttccgaggcg ctgcagccga ccgtgcgccg acccgacggc cgggttctgg ccgatcagga
    11341 aatagccgga gaccttcccg tcgatcatgt ccatgagggt gcgataggtg ccgtggtctc
    11401 cgctgatccg cggcaggtag tcgtagcaga agtcgttctc gggcgtggcc ctcttgcccc
    11461 agtactcctt gagcaaggac accatgtagg cattggcgtt gcaccaaaag cctttctggt
    11521 tctcaccgcc gcagatgctc tccaggtagc tctccaggtc cgggtgagag acggacgagg
    11581 gcatcgccag gtagccggga agcaggttga agagggtcgg gacgtcggtg gagccctgga
    11641 tgctcgcgtg cccccgcagc gccaggatgc cgccgccggg tcggccgatg ttccccagca
    11701 gcaactggat gatcgacccg gcccggatgt actgcgaccc gacgctgtgc tgggtccatc
    11761 cgacgctgta caccagcgcg gatgtgcgct cccttccgga gttggcggtc cacgcctcgc
    11821 acagctcgag gaactgctcc cgaggggtgc cgcagacctc ctcgaccatc tcgggggtgt
    11881 agcgggcgaa gtgtcgcttg aggacctggt agacgcatcg tggatgctgc agtgtcgggt
    11941 ccgaggccat cttcccgagc actccgtcca gcgtcgggcc agccgcaccg tgcgtgtcgc
    12001 cccgcgcgtg ttgcttggcc tccgccggtg cctcctgtcc tggcgagctg ccggtcgggt
    12061 cggccccctc gtagtgccag gtttcggggt cgtaggttgc cgtctcgggg tcgtacccgc
    12121 tgaacagccc gtccaggtcc tcggtgtccc ggaagtcctc gcggatcaga tgtgccgcgt
    12181 tggtgtaggc gaccacgtac tcccggaagt ccagctcgtt gctcaggatg tagttgatca
    12241 ctccgccgag gaaggcgagg tcgctcccgg cgcggagtgg cacgtgcacg tttgccacgg
    12301 cactcgtgcg gctgaaccgc gggtcgacgt ggaagacccg cgtgcccctg gccttggcct
    12361 ccatcaccca ctggaacccc accgggtgtg cttcggccat gttcgagccc atgatgacta
    12421 tgcagtcgga gttgaccagg tcctgctggt agttggtggc gccgccgcgg ccgaaggagg
    12481 ctcccagacc gggaaccgtg gcggagtgtc aaatacgggc ctggttctcg atctggatcg
    12541 cacccagggc ggtgtacagc ttcttgatca ggtagttctc ttcgttgtcc agcgtcgctc
    12601 cgccgaggtg cgcgattccc atcgtgcggc ggagctggtt gccctggtcg tcgtggtcct
    12661 gccagccctt ggcccgggcg tccagcaccc gatccacgac catgtccatg gccgtgtcga
    12721 gctcgagctc gatccagtcg gtggcgtagg gagcccgata gagcaccttc tgctcccgct
    12781 gcgggccggt gaccagctgc ttgctcgccg atcccttggg gcacagccgt ccacgcgaga
    12841 ccgggctgtc gggattgccc tcgatctgga tcactttttc gtcgaaaacg aaaacatcct
    12901 gcgcgcaacc caccgcgcaa tatgggcaaa cggacttgac aactcgatct gcggttgccg
    12961 tgcgtggctg catggcagcc gaaagtggtg actccgcagc tgcaccccgg cccaaccggt
    13021 cgggtccagt cagctggcgg tatatcggcc aggagccgag ccagcgaggc acacccatgc
    13081 gctttctccg tcccggagat caattaacgg ctgctgccgg agcgcgagca caacgagttt
    13141 cctaggacac catgcgaagg caggagatac aaccctcggc gaaccggcta cgggtcacgg
    13201 aagcgccgcc ttcggcgcac gctgcggcag tgagcccggg cgctgcgtac gttggaggtc
    13261 gtggagacgt gggacgcgat cagatctcgg cggaacgtcc gtgtctacga gaatcgaccg
    13321 atcccgcgag aggatctgga gcggatcctg gaagcgggcc gccgcgctcc gtcctcgcag
    13381 aactggcagc cctgggactt cgtcgttgtc accgaccgag agcagctccg gcagctcgcc
    13441 ggggtctggc agggcgcgcg gcacgtggcc gggtccgcag cgacgatcgc gctcgtggcg
    13501 ccgcagcccg aggacgcccg gcagcgcgac tggatccact atgacttcgg ccaggcgacg
    13561 atgaacatga tggtcaccgc ggcggacctc gggatcggca gcggccattc ggcggttgcc
    13621 gaccaggacc tcgcacgccg cctcctcggc aacccggact cgacgtggtg ccagagcctg
    13681 atcgcgctgg gctatcccgc ggaccggccg ctgaccccga tccagcggcc gaaccgccgc
    13741 ccgttcgacg acgtcgtcca ctgggggcgc tggtcgcggg gatgaggccg ggtgcgcgag
    13801 agcgccggga ccgggccagc gcgatcggga cgagtcgcgc ccgcgtcgca aaaccccgtg
    13861 cagcctgtgg gatctcaccg tcgatctcgt ccggaatcgt gatcgaccgg gccgacctgg
    13921 gcggcggctt cagtgggctg gtctgcgacg acccgtgctg cccgactcgc tggacgagac
    13981 caccgtcggc cacgcgcacc tcgacgactg gatcgcccgc gggtactgag cggtggcccg
    14041 aagtttggcg gcgtggcggc acgggaccct gtgcaggtgg agagacggcg gctcggtgtg
    14101 gagggtatgg aggtgccggt catcggcctg gggacgtggc gccggctgga ggcggcggcg
    14161 accggcggcg ggcatgtcgc ggtcgtgacc cgcgccctcg acgcgggggt gcggttcttc
    14221 gattcgtcgc ccatgtatgg ccgggcggag gagttgctcg ccgaggccct gggtgaccgc
    14281 cggggtgaag cggtcgtggc cactaagatc tggacggcct caccggtgga gggacggcgg
    14341 cagctcgacc gggcgctgcg cctctacggc ggtcacgtcg agctgatgca gatccacaac
    14401 ttggtggcct ggcgcgagca cctgccgctg ctgctggcgg cgcgggagga gggccggatc
    14461 gaccggatcg gggcgacgca ttacgcggtg gtccgcgagc tggagacggt gatgcgcacc
    14521 ggtgggatcg acgccgtcca gatcccgtac aacccgcggc agcgggaggc cgagcagcgc
    14581 atcctgccgc tggcggccga cctcggcctc ggcgtcgtcg tgatgcgtcc cttcggcgag
    14641 ggcgagctgc tcgacctcga catcggcgag gcccagctcg cgccgctgta cccgttcggc
    14701 gtccgcacct gggcgcaggc gctgctcaag tgggggctga gcgatccgag gtgcaccgtc
    14761 gcgatcccgg cgacgtcgcg cccgccgcgg atcgacgaga acgtcgccgc gggggattgg
    14821 ccctggttcg gcccggacga gcgggagctc gtccaccggc tggcgctggg cacctgacgg
    14881 cggggccggc gcgccccggc cgatccagcg cgtcggcgcg gcgttgtgct ccctctgcgg
    14941 ggaactgggg caccacgttg tccctccagc tgccgcccgg ggacgtgttc gcgctgcccg
    15001 ccgacacggc gcggcgcagc tctctccgac agacccgtgg gtgcccgaaa ttcggcccac
    15061 gactgtggcc tgcatgtccg ccaggaaccc cggctcggcg tgatcgcccg atgcgctcag
    15121 gctgacgtgc ctgatccttc cccgcctcgt gggctggatg gtgctactcg cccgctccga
    15181 cggggccaaa gacgtcgaga tcctcgtact ggcccgcgcg ggccgcatcg gtcacggtcg
    15241 accccgaacc caatccggga tcagccccag aatccccgat tccgcgccgg gccgcgcatc
    15301 tccgggctac tgcaccgcgg cgggatgcgg gccggtcgcc aaagcgttga gccgatgcgg
    15361 ctcggcgttg atcggtcggt ggccgccacc ttgccgtcgg cgttccacgt gctcaccacg
    15421 ccatgaggaa cgcgctcgcc gaaggtccgg tcttgtagcc aatcaacgcc ggt
//
LOCUS       DASRSC010000152         5223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_869563,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000152 DASRSC010000000
VERSION     DASRSC010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5223
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869563"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..867)
                     /locus_tag="VFE39_07450"
     CDS             complement(34..867)
                     /locus_tag="VFE39_07450"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HET6258470.1"
                     /translation="MVGRSALAGGYVSHGVERVDLDVDGRPVPVVLKQASPVEVAAMR
                     AVMVVPGVDRPRLLAAGSGWLVLPFYDGPALRPEEDVPGEVWTALGRVHAHWWRRRPR
                     GVPVVDAAWWRTMVTRHTLPAVRGARERTGDVLFDEVEALLERWSVEPRLRAALAVLP
                     RTLTHGDAHRGNVLIAADGGVLIDWGNARVAPAGLDLATMAAQGAEPTQAYRAAVGPG
                     STPPELREVERWWARAHVHVQYLGFAADHLGPTRVAEMIETAANAMDRLGPALKSATT
                     R"
     gene            complement(939..1562)
                     /locus_tag="VFE39_07455"
     CDS             complement(939..1562)
                     /locus_tag="VFE39_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET6258471.1"
                     /translation="MTDQPTSILRGSGSSGFGEPESSGTRPTLRPLVWNGGTDIGLLI
                     LRFAVGGTFFAHGMQKVFGMWGGPGVAEFARTLDRYGYQQTTTLSWVTGITELVAGAF
                     VVLGVLTPLAAAALLAIKINAVLLKVASGFFITSVAGANAVELDVVLGLAAAAIIFTG
                     PGRIALDNGRAWHRRPASWGVLALVVGVAAAVLVYVMLRSTPVSGTP"
     gene            complement(1619..2536)
                     /locus_tag="VFE39_07460"
     CDS             complement(1619..2536)
                     /locus_tag="VFE39_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HET6258472.1"
                     /translation="MGDIVVLVPHEEGTAALADVPGITAVAYDPNGELPAAAESAQVL
                     VPPFLATREAVALTRKLPELRLVQLLSAGAENWVGRLPDGVALSDCRGAHGGATAEWI
                     VATLLAVYRHLPRFVRAQDEARWDYHFTDELAGKRVLIVGAGDVAENTEHRLAPFDVE
                     VTLVGRRARDRVHGIDEVLDLLPHHDACVVVVPLTDDTRGLVNAEFLAAMPDGAVLVN
                     AARGPVADTDALTAELTGGRLRAALDVTEPEPLPADHPLWTAPGLLITPHVGASVPGA
                     LRRAYEVVAEQLGALARGERPPNLVEDGY"
     gene            2642..3793
                     /locus_tag="VFE39_07465"
     CDS             2642..3793
                     /locus_tag="VFE39_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET6258473.1"
                     /translation="MGGRVRRAAAAVLLGLLLAGCATFPDQGPREWREQVEGVGELGG
                     PPVVPEPSDPPSAPPGGGAGQPGSPQPPSGCVDPDPQVVATCLEPVGAIAVLPDGRSA
                     LVAERATGRVLRVQRGSEPVLISTVPVDAAGGGGLTGLVLSPSFREDQLIYAYVTTRT
                     DNRVVRIAPGEPPKPVLTGIPRGLRNNGGALGVDVDGSLLVATGDAGGDPTLPGSLAG
                     KLLRIDTLGRPGKGNPDPASRVLSSGLRAPAGICIDPVTTMTWVTDRTGTQDVLHLVV
                     PGPLPAPAWTWPDRPGVAGCMAQPGMVAIALSGGAALFVLHPGDNAAFTGAPEKLLIN
                     TYGRLSAAALAPDGLLWLGTVNKTGGDPVQSDDRVIRIQPPAGGGESRA"
     gene            complement(3863..4840)
                     /locus_tag="VFE39_07470"
     CDS             complement(3863..4840)
                     /locus_tag="VFE39_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258474.1"
                     /translation="MSSETAAALIAARWAAIETRQHGITDVRQLVDLGVTRDTIVAQI
                     EAGRWQWVLPRTYATFTGPIPRPARLVAALRYGGAAAVLSHRTAAEQWGLVPVADGPV
                     HITVPYGTSAISQPPFVIVHRSRAFAYITVALDPPVTSRADTAIDMAAAEPDVRGAQR
                     TLAGLLVTGRVRPLAVERRLLERPPPRYRRALTATVLLVRNGVQSVLEECYAVDLEQA
                     HGLPSGRRQGAVVVDGVTLYEDVMYDEFGADLTVRLDGRTHLLNSVAFRDRRRDNAAE
                     LAGRSRLSYGWHDISDDVCGAAREVVAVLRRRGWDGAAVTCPRCASRGD"
     gene            complement(4973..>5223)
                     /locus_tag="VFE39_07475"
     CDS             complement(4973..>5223)
                     /locus_tag="VFE39_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258475.1"
                     /translation="LCEHNGVPRDTVAEDIGDVADQRGRIVQFAQDLAPTACTSVSQC
                     FALTKDVSETVRVMVKNMDDLSKLDDEYVAPWLGSDRG"
BASE COUNT          719 a         1957 c         1868 g          679 t
ORIGIN      
        1 agtgctccgg cactcgtatc cacgcacgac ctctcagcgt gtggtggccg acttcagcgc
       61 cgggcccagg cggtccatgg cgttcgctgc ggtctcgatc atctcggcca cccgcgtcgg
      121 gcccaggtgg tcggcggcga acccgaggta ctggacgtgg acgtgcgccc gcgcccacca
      181 ccgctcgacc tcgcgcagct ccgggggtgt cgaccccgga cccaccgccg cgcggtacgc
      241 ctgcgtcggc tccgccccct gtgctgccat ggttgcgagg tcgagcccgg cgggcgccac
      301 ccgcgcattg ccccagtcga tgagcacgcc gccgtccgct gcgatcagga cgttgccccg
      361 gtgcgcgtcg ccgtgggtga gagtccgcgg cagtaccgcg agcgcggcgc gcagccgcgg
      421 ctcgacggac caccgctcca gcagcgcctc gacctcgtcg aacagcacgt cgcccgtgcg
      481 ctcccgggcg ccgcgcacgg cgggcagtgt gtgcctggtg accatcgtcc gccaccacgc
      541 ggcgtcgacc accgggacac cgcgcggccg cctgcgccac cagtgcgcgt gcacgcggcc
      601 cagcgccgtc cacacctcgc ccggcacgtc ctcctccggg cgaagtgccg ggccgtcgta
      661 gaacggaagg acgagccagc ccgagcccgc ggcaaggagg cgaggccggt cgacgccggg
      721 caccaccatg acggcccgca tcgccgccac ctcgacgggc gacgcctgct tcagcaccac
      781 gggcaccggg cgcccgtcga cgtcgaggtc gacccgctcg accccgtgcg agacgtaccc
      841 gcctgccagc gcggaccggc ccaccacgcg cggggagcca tgcagcgcgc gaccgaccgc
      901 cggcagccac gcggcgtccg ggaccgttgc gagcggaatc agggtgtgcc ggacacgggt
      961 gtggagcgca gcatcacgta cacgagcacg gccgccgcca ccccgaccac cagggcgagc
     1021 acgccccacg acgcgggtcg ccgatgccag gcgcggccgt tgtccagcgc gatacggccc
     1081 ggccccgtga agatgatcgc ggcggccgcg agcccgagca cgacgtcgag ctcgacagcg
     1141 ttcgcaccgg ccacggacgt gatgaagaac ccgctggcca ccttgagcag caccgcattg
     1201 atcttgatgg cgagcagtgc cgccgccgcg agcggcgtga gcactcccag caccacgaac
     1261 gcgcccgcca ccagctccgt gatcccggtg acccacgaca gggtggtggt ctgctggtac
     1321 ccgtaccggt cgagcgtgcg ggcgaactcc gcgacgcccg gtccgcccca catgccgaac
     1381 accttctgca tcccgtgggc gaagaacgtg ccgcccacgg cgaaccgcag gatcagcagc
     1441 ccgatgtccg tgccgccgtt ccacaccagc ggccggagag tgggccgcgt cccgctcgac
     1501 tccggctcac cgaagccact gctgccggaa ccccggagga tcgacgtcgg ctggtcagtc
     1561 atgccccggg agggtagacc cgacggacga tcagcgctgg tgagttgacc gatctgggtt
     1621 agtacccgtc ctcgaccagg ttcggcgggc gctcgccgcg ggcgagcgcg ccgagctgct
     1681 cggcgaccac ctcgtaggcg cgccgcagcg caccggggac gctggccccg acgtgtggcg
     1741 tgatcagcag gccgggggcg gtccacagcg ggtggtcggc gggcagcggc tcgggctccg
     1801 tgacgtcgag cgcggcgcgc agccgcccgc ccgtcagctc cgcggtgagc gcatcggtgt
     1861 cagcgacggg tccgcgcgcg gcgttgacga gcaccgcccc gtccggcatt gcggcgagga
     1921 actcggcgtt cacgagaccg cgggtgtcgt cggtcagggg tacgaccacc acgcaggcgt
     1981 cgtggtgggg cagcaggtcg agcacctcgt cgatgccgtg caccctgtcg cgggcgcggc
     2041 ggccgaccag cgtgacctcg acgtcgaacg gcgcgaggcg gtgctccgtg ttctccgcga
     2101 cgtcgcccgc ccccacgatg agcacccgtt tgccggccag ctcgtcggtg aagtggtagt
     2161 cccagcgcgc ctcgtcctgc gcccggacga agcgcgggag gtgccggtac acggcgagga
     2221 gggtcgcgac gatccactcc gccgtggcac cgccgtgggc accgcggcag tccgagagcg
     2281 cgacgccgtc gggcagcctg ccgacccagt tctccgcccc cgccgagagc aactgcacga
     2341 gccgcaactc cggcagcttc cgcgtcaggg ccaccgcttc tcgggtcgcg aggaacgggg
     2401 gcaccagcac ctgtgccgac tcggccgcgg caggaagctc cccgttgggg tcgtacgcca
     2461 ccgcggtgat gccggggacg tcggcgagcg cggccgtccc ctcctcgtgc ggaaccagaa
     2521 caacgatgtc gcccatgagc gtgaccctac gacgcgtcca ggccccgttc cgcgacgccg
     2581 tagggaatgc ggcagcccgc tactcaccgg ccgctcaccc cgtccccctt agcctgggcc
     2641 cgtgggagga cgggtgcggc gcgctgcggc cgcggtgctg ctcggcctgc tgctggcagg
     2701 ctgcgcgacg ttccccgacc agggcccgcg ggagtggcgg gagcaggtgg agggcgtcgg
     2761 cgagctcggc ggcccaccgg tcgttcccga gccgagcgac cccccgtccg ctccgccggg
     2821 tgggggcgcc gggcagccgg gctcgcccca gccaccgtcg ggctgcgtcg atcccgaccc
     2881 gcaggtcgtc gcgacctgcc tggagccggt cggcgccatc gcggtgctgc cggacgggcg
     2941 gtcggcgctc gtggccgagc gcgccaccgg acgggtgctg cgtgtgcagc gtggttcgga
     3001 gccggtcctg atctccaccg tgccggtgga cgccgctggt ggcggcggcc tgaccgggct
     3061 cgtgctctct ccgtcgttcc gcgaggacca gctcatctac gcctacgtca ccacccgcac
     3121 ggacaaccgg gtggtgcgca tcgctcccgg cgagcccccc aaacccgtgc tcaccggtat
     3181 ccctcgcggg cttcggaaca acggcggagc gctcggtgtc gacgtcgacg gatcgttgct
     3241 ggtggccacg ggtgacgccg ggggtgatcc gaccctgcca ggttcgctcg cgggcaagct
     3301 gttgcggatc gacacgctcg gtcgccccgg caagggcaac ccggaccccg cctcccgggt
     3361 gctcagctcc gggctgcgcg cgcccgccgg catctgcatc gaccccgtca cgacgatgac
     3421 ctgggtaacc gaccggaccg gcacccagga cgtgctgcac ctcgtcgtac cagggccgct
     3481 tcccgcgccc gcctggacct ggcccgaccg ccccggcgtc gccggctgca tggcccagcc
     3541 ggggatggtg gcgatcgcgc tcagcggcgg ggccgccctg ttcgtgctgc accccgggga
     3601 caacgccgcg ttcaccggcg cccccgagaa gttgctcatc aacacctacg gccgactgtc
     3661 ggcggccgcc ctcgccccgg acgggctgct ctggctgggc accgtcaaca agacgggcgg
     3721 cgatccggtg cagagcgacg accgcgtcat ccggatccag ccgcctgcgg gaggcgggga
     3781 gagccgcgcc tgacggcgcc cgtgcgcgga tacgagtgct ggagcactcg tatccgcgca
     3841 cgagagctca cccgcgggcc ggtcagtcac cccggcttgc gcagcgcgga catgtgaccg
     3901 cagcgccgtc ccacccgcgg cgccggagta ccgctacgac ctcgcgagcg gcgccgcaca
     3961 cgtcgtcgga gatgtcgtgc cagccgtagg acaggcgcga ccgcccggcg agctcggcgg
     4021 cgttgtcacg ccggcgatcc cggaacgcca cgctgttcag aaggtgcgtc cggccgtcca
     4081 gccgaactgt gaggtcggca ccgaactcgt cgtacatcac atcctcgtag agggtgacgc
     4141 cgtccacgac gaccgctccc tgcctgcgcc cggacgggag gccgtgcgcc tgctccaggt
     4201 cgaccgcgta gcactcctcg agcacggact gaaccccgtt gcggaccagc aggacggtcg
     4261 ccgtcagcgc ccgccgatag cgtggcggcg ggcgttccag cagcctgcgc tccaccgcga
     4321 ggggtcggac ccgcccggtg acgaggagcc cggcgagcgt gcgttgcgcg ccacggacgt
     4381 cgggctcggc ggccgccatg tcgatcgccg tgtccgcacg gctggtgacc ggtgggtcga
     4441 gcgcgaccgt gatgtacgcg aaggcccggg accggtgcac gatcacgaac ggcggctgcg
     4501 agatcgccga tgtgccgtac ggaacggtga tgtgcacggg cccgtcggcg accgggacga
     4561 ggccccactg ctcggctgcc gtgcgatggc tgaggaccgc cgctgcgccc ccgtaccgga
     4621 gcgccgcgac gagccgcgcc gggcgcggga tgggtccggt gaacgtggcg tacgtccggg
     4681 gaagcaccca ctgccaccgg cccgcctcga tctgggcgac gatcgtgtcg cgcgtgacgc
     4741 cgagatcgac gagctggcgg acgtcggtga tgccgtgctg gcgcgtctcg atcgccgccc
     4801 agcgtgcagc gatgagcgcc gcggctgtct cggaggacat gccgcgagcg tcccggacgg
     4861 acccgcgccg cgggcccgga tgctccaggc tgtggacagc ttcccgatag tcgtgcgcgg
     4921 atacgagtgc tggagcactc gtatccgcgc acgggcagtt ggcgcgccgg atctatcccc
     4981 ggtcgcttcc cagccaggga gcgacgtatt cgtcgtcgag cttgctcagg tcatccatgt
     5041 tcttgaccat gacgcgcact gtctccgaga cgtccttggt cagcgcgaag cactggctga
     5101 ccgaggtgca ggcggtcggc gcgaggtcct gcgcgaattg gacgatccgc ccacgctgat
     5161 cggcgacgtc accgatgtcc tcggccacgg tgtcgcgcgg aacaccgttg tgttcacaca
     5221 gag
//
LOCUS       DASRSC010000153         3533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5456927,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000153 DASRSC010000000
VERSION     DASRSC010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5456927"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2831)
                     /gene="drmA"
                     /locus_tag="VFE39_07480"
     CDS             complement(<1..2831)
                     /gene="drmA"
                     /locus_tag="VFE39_07480"
                     /inference="COORDINATES: protein motif:HMM:NF038325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DISARM system helicase DrmA"
                     /protein_id="HET6258476.1"
                     /translation="MTTPEVTSFQVRAELEDRLARDLLGPWDGPEEELPPGTSPAERY
                     MLGRLVPRERPAAAEDPDEGEAVDAEETDPGLVDREVTAAPDGEEEGAEAEATIRSGS
                     MAASAIGLAFSVPATIDVISVVAKWGRYERSPSETHETPQGRAAITWHRVPTGGAVEV
                     RTDAEGTGEVVPDDEQPGVVIRWTVRHRGARRVVELSLVNGQRAPARAQDTARLYQVG
                     LTVTALDGDAAIFLGHNDPSVAPPPSAFDDERLHLELLHRGTRQYAHGRQCAVDAFWR
                     AGETQAWKLATTCFPAAEVPLVVPGRVPGLVLDMARLGSPELGRDDLVKALRPLVTGY
                     RRWLAEQASRVESDPEVGRYAPAGEHAVVAARAVADRLERAIELLRGDGIAREAFRFA
                     NQAMALQRVRSEVVRARMSSPGADVSPLLREKDVPGNRSWRPFQLAFVLLCLPGLTNP
                     SHPDAHRGLGSPDDAEVQLLFFPTGGGKTEAYLGLAAYTFAIRRLQGVLGAGSSDAVD
                     GTRGVAVLMRYTLRLLTAQQFQRASALLCGCEMLRRERLDAGDSRWGTTPFRIGLWVG
                     STVSPNSFEEAERQITETRGNERLLGGVLQLPVCPWCGSGLSAGRDVVTDRLRRRVLV
                     YCSDPEGDCAFSPRNSPGEGLPVLTVDEEIYRLTPALVIATVDKLAQLPWKAATASLF
                     GLIDTECTRHGWQNPEFSSFCGPGGHPARGELPRAVIQPAMRLRPPDLIIQDELHLIS
                     DALGSMVGLYETAIDRLCSRGPVRPVLVASTATVRRARDQVEQVFARGLTVFPPPLLD
                     AGETYFSATVPPTASAPGRRYRGVLAPGERLKSVEIRVASALLEHAQFLFDRYGSAAD
                     PYMTLVDYFTSTRELAGMRRLVEDDVADRLSSRQVRTRRKRPRVDELTGRMPSSRIAE
                     SLAELEHPFNPSFDSTAALDALRQNPEEYR"
     gene            complement(2828..3385)
                     /locus_tag="VFE39_07485"
     CDS             complement(2828..3385)
                     /locus_tag="VFE39_07485"
                     /inference="COORDINATES: protein motif:HMM:NF042871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HET6258477.1"
                     /translation="MIVIAQTSSTIMPVIAPTNQVFDQSCIVFARNDFGTLAILSSSL
                     HSAWVMRYTTPMRNDIRYLPAHTFATFPRPRVSPNLETLGQQLNTMRSELMQSRAWGL
                     TKTYRQVHNPDIHERAIEELRDIHVAIDEAVTQAYGWDDLDLKIGHHPTKIGVRWTVS
                     KEARFELLDRLLEENHQRYKLENPS"
BASE COUNT          561 a         1312 c         1104 g          556 t
ORIGIN      
        1 cggtactcct cggggttctg ccggagcgcg tcaagcgcgg ccgtcgagtc gaacgacggg
       61 ttgaaggggt gctccagctc ggcgaggctc tcggcgatcc ggctggacgg catccgcccg
      121 gtcagctcgt ccacccgtgg gcgcttccgc cgggtgcgca cctgccgcga cgacagccgg
      181 tccgcaacgt cgtcctccac gaggcgccgc atgccggcca gctcgcgggt ggaggtgaag
      241 tagtccacca gcgtcatgta ggggtccgca gccgagccgt accggtcgaa caggaactgc
      301 gcgtgctcaa gcagcgccga cgccacccgg atctccaccg acttcagccg ctcgcccggc
      361 gcgagcacgc cccggtaccg tcgtcccggc gcggaagcgg tcggcggcac cgtcgcggaa
      421 aagtacgtct cgcccgcgtc aagcagcggc ggcgggaaca ccgtaagacc gcgtgcgaac
      481 acctgctcca cctggtcccg tgcccgccgt acggtcgcgg tggaggcgac cagcaccggc
      541 cgcaccggcc cccgcgagca cagccggtcg atcgccgtct cgtacagccc gaccatcgac
      601 ccgagtgcgt cgctgatcag gtgcagctcg tcctggatga tcaggtccgg cggccgcagg
      661 cgcatcgccg gctggatcac cgcacggggc agttccccgc gcgccgggtg cccgcccggc
      721 ccgcagaacg acgagaactc ggggttctgc cacccgtgcc gcgtgcactc cgtgtcaatg
      781 agcccaaata gagaagcggt agccgccttc cacggcagct gcgccagctt gtccacggtc
      841 gcgatgacca gcgcaggggt cagccggtag atctcctcgt ccacggtaag caccggcagc
      901 ccctcgccgg gagagttgcg cggcgagaac gcgcagtccc cctccgggtc cgagcagtac
      961 acaagcaccc gccgccgcag ccggtcggtt accacgtccc gcccggcgga caaccccgac
     1021 ccgcaccacg ggcacaccgg caactgcagc accccgccga gcagccgctc gttgccgcgc
     1081 gtctcggtga tctgccgttc ggcttcctcg aacgagttcg gcgacaccgt cgacccgacc
     1141 cacagcccga tccggaacgg cgtcgtgccc caccgcgagt ccccggcatc caggcgctcc
     1201 cggcgcagca tctcgcaccc gcacaacagc gccgaagccc gctggaactg ctgagcggtc
     1261 agcagccgca gcgtgtaccg catcagcacc gccaccccgc gcgtcccgtc gaccgcatcc
     1321 gaagagcccg cgccgagcac cccctgcaac cgccggatcg cgaacgtgta cgccgcgagc
     1381 cccaggtacg cctcggtctt accgccaccg gtcgggaaga acagcagctg cacctcggcg
     1441 tcatccggtg aaccaagccc ccggtgcgcg tccggatgcg acgggttggt cagccccggc
     1501 aggcacagca gcacgaacgc gagctggaac ggccgccatg accggttccc aggcacgtcc
     1561 ttctcccgca gcagcggcga aacgtcagca ccaggcgacg acatccgcgc ccgcacgacc
     1621 tccgaccgca cgcgctgcag cgccatcgcc tggttcgcga accgaaacgc ctcccgcgcg
     1681 atcccgtcgc cgcgtagcag ctcgatcgcc cgctccaacc ggtccgccac cgcccgcgcc
     1741 gccaccaccg cgtgctctcc ggcgggcgcg taccgcccga cctccgggtc agactccacc
     1801 cgcgacgctt gctcggcgag ccagcgacgg tacccggtca ccagcggccg cagcgccttg
     1861 accaggtcgt cgcgcccgag ctccggcgac ccgagccgcg ccatgtcaag cacaagaccc
     1921 ggcacgcgac cgggcacgac caggggcact tccgccgcgg ggaagcacgt cgtggccagc
     1981 ttccacgcct gcgtctcgcc cgcccgccag aacgcgtcca cggcgcactg ccgcccgtgc
     2041 gcgtactgcc gggttccccg gtgcagcagc tccaggtgca gccgctcatc gtcgaaggcc
     2101 gacggcggcg gcgcgaccga cgggtcgttg tgcccgagga agatcgccgc gtccccgtcc
     2161 agggcagtca cggtcagccc gacttgatac agccgcgccg tgtcctgagc tctcgcgggt
     2221 gctcgctgcc cgttcaccag cgacagctca acaacccgcc gcgcaccccg gtgccgcacc
     2281 gtccaccgga tgaccacgcc cggctgctcg tcgtccggca cgacctcgcc ggtcccctcg
     2341 gcgtccgtcc gcacctccac ggctccgccc gtcggcaccc ggtgccaggt gatcgcggcg
     2401 cgcccctgcg gcgtctcatg ggtctccgac ggcgaccgct cataccgccc ccacttggcg
     2461 accacgctga tcacatcgat ggtcgctggg acgctgaaag ctagtccgat ggctgaggcc
     2521 gccattgatc ccgaacggat ggtggcttct gcctcggccc cctcttcctc tccgtccggc
     2581 gcggccgtga cctcccggtc caccagccct ggatcggtct cctcggcatc gacggcctcg
     2641 ccttcgtcgg ggtcttctgc tgcggcgggc cgctccctcg gcacgagccg tccgagcatg
     2701 taccgctccg ctggcgaggt gcccggcgga agctcctcct cggggccgtc ccaggggccg
     2761 aggaggtctc gtgcaaggcg gtcctctagc tcggcccgga cctggaacga ggtgacctcg
     2821 ggtgtcgtca cgacgggttc tccagcttgt agcgctggtg gttctcttcg aggagtcggt
     2881 ctaggagctc gaaccgcgcc tccttgctta cggtccaccg gacgccgatc ttggtggggt
     2941 gatggccgat tttgaggtct aggtcgtccc agccgtaggc ctgtgtgacg gcttcgtcaa
     3001 ttgcgacgtg tatatcgcgg agttcttcaa tcgcgcgctc atggatatcg ggattgtgga
     3061 cttgcctgta ggtcttggta agaccccagg cgcgagactg catcagttcg ctgcgcatgg
     3121 tgtttagttg ctgtccaagt gtttcgagat ttggactcac cctgggccgc ggaaatgtcg
     3181 cgaacgtgtg ggccggcaaa tacctgatgt cgtttcgcat tggagttgta taacgcatta
     3241 cccatgccga atggaggctt gatgaaagga ttgccagagt cccgaaatca ttgcgggcga
     3301 agacgataca cgattgatcg aatacctgat ttgttggtgc gataacaggc attatcgtgc
     3361 tactagtttg tgcaattacg atcacctggc tcagtccaac gagagccttg actagcccgg
     3421 gtcgatgttc gccataccgc caccagatct cgcgatagtt gacacgatta tttcggtctc
     3481 gtccaggctt gactagtcgc cggacaatgg caatacaatc agggtactct tca
//
LOCUS       DASRSC010000154         4255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3653247,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000154 DASRSC010000000
VERSION     DASRSC010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3653247"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /gene="prs"
                     /locus_tag="VFE39_07490"
     CDS             <1..624
                     /gene="prs"
                     /locus_tag="VFE39_07490"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET6258478.1"
                     /translation="IADMFQVAGADRIMTVDLHTAQIQGFFDGPVDHLFALPVLAEHV
                     KRTYAEHPLAVVSPDSGRVRLAERWAETLGGTPLAFIHKTRDARRPNEAVANRVVGEV
                     EGRLCVVIDDMIDTGGTVAKTVEVLLKEGALDVVVAATHGVLSGPAPERLATCGAREV
                     IFTDTLPIPDEKRFPTMTVLSIAPLLARAIHEVFDDGSVTSLFDGNA"
     gene            947..1585
                     /locus_tag="VFE39_07495"
     CDS             947..1585
                     /locus_tag="VFE39_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HET6258479.1"
                     /translation="MAEARIDAETRTEFGKGAARRTRRAGKIPAVLYGHGTDPQHLSL
                     PELEFKRVVREQGRNAVLTLNIGGTPQLALTKTVVTHPIRPYIEHVDLLVISRGEKVA
                     VEVQIAIVGDAAPGTLVTQELNALEVEADVSNIPEQIEVSVEGLPVGTQIQASDVPLP
                     EGTTLRAEPEILVVNIVAAPTEAQLEAEVDTEGAGVVEDAPETPAAAASPES"
     gene            1582..2172
                     /gene="pth"
                     /locus_tag="VFE39_07500"
     CDS             1582..2172
                     /gene="pth"
                     /locus_tag="VFE39_07500"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET6258480.1"
                     /translation="MTVAPGPALVIGLGNPGPDYAETRHNVGFRVVELLAARAGGGRF
                     SKHRTNADVLEGRLSGRRVVFAKPRTYMNVSGGPVAGLAKYFSVPLEDLVVVHDDLDL
                     GFGVVRLKRGGGEGGHNGLRSISSAVGTKDYLRVRFGIGRPPGRQDPADFVLKRFSGA
                     EKKELEFAVDLAADAAEALLADGLEPAQNRFHALSG"
     gene            complement(2210..3883)
                     /locus_tag="VFE39_07505"
     CDS             complement(2210..3883)
                     /locus_tag="VFE39_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HET6258481.1"
                     /translation="MSRFLALLLDSAVRSSRGMTTGEPRTAVRRTWPDVHAAARRMAA
                     SLRSGDGEVPGLQHGAAVGILAGEPASIAPVAQAVWITGGSVTMLHQPTARTDLAAWA
                     EDTMSVLRMIDAKLVLLGAPFDALAPVLTERGVAFRTIDSLTTTGEDIDPDAEVAGED
                     DTALLQLTSGSTAEPKAVRITHRNLHANIAGMVRASALDVERDVMVSWLPLFHDMGMV
                     GFLTVPMATGLELVSVTPVDFLTRPVLWAELISKYGGTVTAAPNFAYAVLGRQLARAD
                     DGSLDLSSLRIALNGAEPIDPDAVEAFSAAGSRFGLRPDSVVAAYGMAETALGVSFAP
                     IDTGLQVDHIDAEQLEAQRRAVPAGDGPRRRFPKLGPPLPGIEVRVVGDDGSVLGERE
                     VGVLQLRGDSVTPGYLTVDGPVPTQDPEGWLDTGDEGYVVDGAVVVCGRRKDVIIMGG
                     RNIYPTDIERAAADADGVRAGNVVAVRMLTGTEGARHRESFLVAVESRSAGDPEAEKL
                     IRKDVASRVVSAVAVRTAEVVVLGPGSLPKTPSGKLRRAATGELLRARV"
     gene            4061..>4255
                     /locus_tag="VFE39_07510"
     CDS             4061..>4255
                     /locus_tag="VFE39_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258482.1"
                     /translation="MAQPHGPEASLEIGGKVSEWDAADESLKSFAHMAVAAQVGCSWC
                     LDIGYFPAQNQNLDMAKASQV"
BASE COUNT          634 a         1562 c         1486 g          573 t
ORIGIN      
        1 atcgccgaca tgttccaggt cgcaggcgcc gaccggatca tgacggtcga cctgcacacc
       61 gcgcagatcc agggcttctt cgacggcccg gtcgaccacc tcttcgcgct gccggtgctc
      121 gccgagcacg tcaagcgcac ctacgccgag cacccgctgg ccgtcgtgtc ccccgactcc
      181 ggccgtgtcc ggctggccga acgctgggcc gagaccctgg gcggcacgcc gctggccttc
      241 atccacaaga cccgcgacgc gcgcaggccc aacgaggccg tcgccaaccg cgtcgtcggc
      301 gaggtcgagg gcaggctctg cgtcgtgatc gacgacatga tcgacaccgg tggcacggtg
      361 gcgaagaccg tcgaggtcct cctcaaggaa ggcgccttgg acgtcgtcgt cgcggcgacg
      421 cacggggtgc tctccggccc ggcgcccgag cgcctcgcca cctgcggagc acgcgaggtc
      481 atcttcaccg acacgctccc gatcccggac gagaagcggt tcccgacgat gacggtgctg
      541 tccatcgccc cgctactggc ccgcgccatc cacgaggtct tcgacgacgg ctcggtcacg
      601 agcctgttcg acggcaacgc ctgacgcgcg tcagggctgt gcgaagaggc cggtaggccc
      661 ctcggggtcc tccggccgca cgggctacgc cggctcggct ggtcccgtcc cctggtgggg
      721 gcgccgccga gcacccgcca ggagcaaagc accggcgacg aggcaggcgg cgcggaggac
      781 aaccccggcc gtcgggcgcc acccgtggat cgcgtatcgt gaacgccagt tccgcggcga
      841 gggcgggtgt ccttctagcc cgccgttatc gacgcagccc cggcccggcc ggcctgcctc
      901 gccgcgcgcg tccatgtcgc agcacgcttg atcgaggaga agtaccgtgg ccgaggcccg
      961 tatcgacgcc gagacccgca cggagttcgg caagggcgcc gcccgccgca cccgtcgcgc
     1021 cggcaagatt cccgctgtgc tctatgggca cggcaccgac ccgcagcacc tctcgctccc
     1081 ggagctggag ttcaagcggg tcgtccgtga gcagggccgc aacgcggtcc tgacgctgaa
     1141 catcggcggc acgccccagc tggcgctgac caagaccgtc gtgacccacc cgatccgccc
     1201 gtacatcgag catgtcgacc tgctggtcat cagccggggc gagaaggtgg cggtcgaggt
     1261 ccagatcgcc atcgtcggcg atgcggcgcc cggcaccctc gtcacgcagg agctcaacgc
     1321 tctcgaggtc gaggccgacg tctcgaacat cccggagcag atcgaggtgt ccgtcgaggg
     1381 gctgccggtc ggcacccaga tccaggcctc cgacgtgcca ctgccggagg gcaccaccct
     1441 gcgcgccgag cccgagatcc tcgtggtcaa catcgtcgcc gccccgaccg aggcccagct
     1501 cgaggccgag gtcgacaccg agggcgccgg cgtcgtcgag gacgccccgg agaccccggc
     1561 ggctgctgcc tcgcccgaga gctgaccgtg gcgccgggcc ccgctctggt gatcgggctc
     1621 ggcaacccgg gcccggacta cgccgagacc cggcacaacg tcggttttcg cgtcgtcgag
     1681 ctgctcgcgg cgcgggccgg cggtggccgg ttctccaagc atcggacgaa cgccgatgtg
     1741 ctcgagggca ggctctccgg gcggcgggtc gtgttcgcga agccgcgcac gtatatgaac
     1801 gtctccggcg gccccgtcgc cgggctcgcg aagtacttct cggtgccgct cgaggatctt
     1861 gtcgtcgtcc acgatgacct ggacctcgga ttcggcgtgg tccggctcaa gcgcggcgga
     1921 ggcgagggcg gccacaacgg gctgcgctcg atcagctccg cggtcgggac gaaagactat
     1981 ctgcgcgtcc ggttcgggat cggccggccc ccgggccgcc aggatccggc cgacttcgtg
     2041 ctgaagcgct tctcgggggc cgagaagaag gagctggagt tcgccgtcga cctggcggcc
     2101 gacgccgccg aggccctgct cgccgacggt ctcgaaccgg cgcagaacag gttccacgca
     2161 ctgtccgggt gagtcgtcct gtcgcggccg gcccgcgggc ccgccgacgt cagacgcggg
     2221 cccgcaacag ctccccggtc gcggcgcggc gcagcttgcc cgacggcgtc ttgggcaggc
     2281 tgccgggccc gagcaccacg acctcggcgg tccgcaccgc caccgcggag accacgcggc
     2341 tcgcgacgtc cttgcgaatc agcttctcgg cctccgggtc gccggcgctg cgcgactcga
     2401 ccgccacgag gaacgattca cgatgccgcg cgccctccgt accggtgagc atccggacgg
     2461 ccaccacgtt gcctgcgcgc acaccgtcgg cgtcggccgc ggcgcgctcg atgtcggtcg
     2521 ggtagatgtt gcgaccaccc atgatgatca cgtccttgcg gcggccgcac accacgacgg
     2581 caccatcgac gacgtaaccc tcgtcgccgg tgtcgagcca gccctcgggg tcctgcgtgg
     2641 ggaccggccc gtcgacggtg agatagccgg gcgtcaccga gtcgccccgc agctgcagca
     2701 caccgacctc gcgctcgccc aataccgagc cgtcgtcgcc gaccacccgg acctcgatgc
     2761 cgggcagcgg cggcccgagc ttcggaaagc ggcgtcgtgg cccgtcaccg gccgggaccg
     2821 cacgccgctg cgcctccagc tgctctgcgt cgatgtggtc gacctgcaac ccggtgtcga
     2881 taggcgcgaa ggacacaccg agcgccgtct cggccatccc gtacgcggcg accaccgagt
     2941 ccgggcgcag accgaagcgg ctccccgccg ccgagaatgc ctccacggca tcgggatcga
     3001 tcggctcggc cccgttgagc gcgatccgca gcgacgagag gtcgagcgac ccgtcgtcgg
     3061 cacgcgccag ctgccggccg agcaccgcgt aggcgaagtt cggcgccgcc gtgaccgtgc
     3121 cgccgtactt cgagatcagc tcggcccaca gcaccgggcg ggtgaggaag tcgaccgggg
     3181 tgacgctcac gagctcgagc ccggtggcca tcgggaccgt gaggaacccc accatgccca
     3241 tgtcatggaa cagcgggagc caggacacca tcacgtcccg ctcgacgtcc agcgcggacg
     3301 cccggaccat gccggcgatg ttggcgtgca ggttgcggtg ggtgatccgc acggccttcg
     3361 gctccgccgt cgagccactg gtcagctgca ggagggcggt gtcgtcctca cccgcgacct
     3421 ccgcgtccgg gtcgatgtcc tcgccggtcg tggtgaggga gtcgatggtg cggaacgcga
     3481 ccccgcgctc ggtgagcacc ggcgccagcg cgtcgaacgg cgcaccgagc agcacgagct
     3541 tcgcgtcgat catcctgagg acggacatgg tgtcctccgc ccaggccgcg agatccgtgc
     3601 gggctgtggg ctggtggagc atcgtgacgc tgccgccggt gatccacacg gcctgcgcca
     3661 cgggcgcgat cgacgcgggc tccccggcga ggatgccgac cgccgcaccg tgctgcagac
     3721 cgggcacctc gccgtcgcct gagcgcaggg aggccgccat gcgccgggcg gccgcgtgca
     3781 catcgggcca ggtgcgccgg accgccgtcc tcggctcgcc ggtcgtcatc ccacgggagg
     3841 aacggacggc ggagtccagc aacagcgcca ggaaccggga catggtcaag agcttacggt
     3901 ctctgccgag agctgacctc ccgaccggaa agggcgatat cgggctcagg gcggattcga
     3961 cgtccgcgac caggacgtgg cccgcctctc gccgttcgtg cgggctcagc ggaagcatga
     4021 tcaagcagct cggtgccgtg cccgaacccg tcgaggtgat gtggcacaac cccacggtcc
     4081 cgaggcctcc ctggagatcg ggggcaaggt gagcgagtgg gatgcggccg acgagagcct
     4141 caagtcgttc gcgcacatgg ccgtcgcggc acaggttggc tgcagttggt gcctcgacat
     4201 cggctacttc ccggcgcaga accagaacct ggacatggcc aaggcgagcc aggtg
//
LOCUS       DASRSC010000155         2523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4146666,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000155 DASRSC010000000
VERSION     DASRSC010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4146666"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..451)
                     /locus_tag="VFE39_07515"
     CDS             complement(98..451)
                     /locus_tag="VFE39_07515"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HET6258483.1"
                     /translation="MASRARLATLDYDFDRLAALMGARGWTTVALVHRSDDRTFHARN
                     PFPPGGVVEDPATGAAAAAFGGYLRELGLVTPPVTLTVHQGDDMRRPSLLTVDVPAGP
                     ESGIAVSGAAVPLAD"
     gene            complement(414..947)
                     /locus_tag="VFE39_07520"
     CDS             complement(414..947)
                     /locus_tag="VFE39_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis isomerase"
                     /protein_id="HET6258484.1"
                     /translation="MSPEILRWSAFTTVPEGGNPAGVVLDARGLDDVEMQRIAADVGY
                     SETAFLQPGIEPGDVTVRYFSPLAEVPFCGHATIASAVAHARRHGPGLLRYATPAGPV
                     QVQTEAGADGTVTATLVSVPPRTVEIAADDLDELLAALRWSRPALDPGLPPRVAFAGV
                     APRRGRGQPRAARHAGL"
     gene            complement(951..1157)
                     /locus_tag="VFE39_07525"
     CDS             complement(951..1157)
                     /locus_tag="VFE39_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258485.1"
                     /translation="MHAFRLNGSLVLEIDLRGDARAYDLWIHDRLHRRRDFPERRRGE
                     ILGQPDRTHLKGVIQARLAQQQGR"
     gene            1381..1836
                     /locus_tag="VFE39_07530"
     CDS             1381..1836
                     /locus_tag="VFE39_07530"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6258486.1"
                     /translation="MEQDVPGHVEFVHRSWPADPEQLVLIRRELGGWLAPLALTEEEI
                     ADVVLAVDEAAANAVRHAYGPNESGAVELTLWTEPGTLSIEVVDHGHWRPPAEERTQG
                     GHGIPLMSMMSESVLIHFDGRGSRVLLRHRIPPGESSAPQPPHGQPPQP"
     gene            1904..2188
                     /locus_tag="VFE39_07535"
     CDS             1904..2188
                     /locus_tag="VFE39_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6343 family protein"
                     /protein_id="HET6258487.1"
                     /translation="MARDRPRRTRADYERGLPGYHDPTTGFGGAAPARSALTLRAVLA
                     VFGLVFAAGAAWLGFAAGLPVLGWVMVAVAVVAIGDLVWVLYRKRRGEPG"
     gene            complement(2185..>2523)
                     /locus_tag="VFE39_07540"
     CDS             complement(2185..>2523)
                     /locus_tag="VFE39_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET6258488.1"
                     /translation="EHVEHYLGLFDGKELADAWTTSEDVQRTKPAPDLLEAALRAVDG
                     SAGVMIGDSVWDFRAAAALSAPGYAVRSGGFSVDELCAAGALGVFDSPAELLHHLDET
                     LLSRSGQRVS"
BASE COUNT          351 a          868 c          967 g          337 t
ORIGIN      
        1 cttcgtcctg gccatcgcga tgatcggcct aggcgtcctg ctcgggcgcg acttgcgggc
       61 cggcgcgcgg ggaggcttgc ggcggtgagt gccggcgtca gtcggcgagc ggcaccgccg
      121 cgccggagac ggctatcccg ctctccggac ccgccgggac gtcgacggtg aggaggctcg
      181 ggcgccgcat gtcgtcgccc tggtgaacgg tgagcgtcac cggtggggtg accaggccga
      241 gctcccgcaa gtagccgccg aacgcggcgg ccgccgcccc ggtggccgga tcctccacca
      301 caccgccggg cgggaacggg ttgcgggcgt ggaaggtgcg gtcgtccgac cggtgcacca
      361 gcgcgacggt cgtccagccg cgcgcgccca tcagggcggc gagccggtcg aagtcatagt
      421 ccagcgtggc gagccgcgcg cggctggcca cgcccacgac ggggtgccac cccggcgaag
      481 gcgacgcgcg gcgggaggcc ggggtcgagg gcagggcgcg accagcgcag cgcggccagc
      541 agctcgtcga ggtcgtcggc ggcgatctcg accgtgcgcg gtggcacgct caccagcgtg
      601 gcggtgaccg tcccgtccgc gcccgcctcg gtctgaacct gcacgggccc ggccggggtg
      661 gcgtagcgga gaaggcccgg gccatgccga cgggcgtgcg ccacggctga ggcgatcgtg
      721 gcgtgcccgc agaacggcac ctcggccagc gggctgaagt agcggacggt cacgtcgccg
      781 ggttcgatgc cgggctgcag gaacgcggtc tccgagtagc ccacgtcggc cgcgatgcgc
      841 tgcatctcca cgtcgtcgag gccccgcgcg tccagcacga ccccggccgg gttaccgccc
      901 tcgggaaccg tggtgaacgc ggaccagcgc agtatctccg ggctcatcgc ttaccgaccc
      961 tgctgctgtg cgagccgggc ctggatcacg cccttcaggt gggtgcggtc gggctggccg
     1021 aggatctccc cgcgccggcg ttccggaaag tcgcgtcgcc ggtgtaggcg atcatggatc
     1081 cagaggtcgt acgcacgtgc atcgcccctc aggtcgatct ccagcaccag ggacccgttc
     1141 agccggaagg cgtgcacgcg cggatcttgg cacacgctga gcacggggga cgagggctgg
     1201 ggaaggtttc ggcgcccgtg atcgggtaac aggccgacgt gggcgcacga acctcaggtg
     1261 cgtcgaccat gcgaccgggc tacggtgcga ccggtcattg tgcacgtggg gcgagccggg
     1321 tgagccggac gagactgagc cgggtcctgt tcgggggcgg acgcggagga atagaggatc
     1381 atggagcagg acgtccccgg gcacgtcgag ttcgtgcacc ggagctggcc ggccgatccg
     1441 gagcagctgg tgctgatccg gcgcgagctg ggcggctggc tcgcgcccct ggcgctcacc
     1501 gaggaagaga tcgcggacgt cgttctcgcg gtggacgaag ctgccgcgaa cgcggtgcgg
     1561 cacgcctacg ggccgaacga gtccggtgcg gtcgagctga cgttgtggac cgaaccaggg
     1621 acgctcagca tcgaggtggt cgaccacggc cactggcgcc cgcctgcgga ggagcggaca
     1681 cagggcggcc acggtatccc gttgatgagc atgatgtcgg agtccgtcct catccacttc
     1741 gacggccgcg gaagccgggt gctgctccgc caccgcatcc cgcctgggga atcatcggct
     1801 ccacagccac cgcatggtca gccaccgcag ccgtagccgc cgctctgacg aattccgggc
     1861 ccgaagactc gtgacggcgc cgcccggcgg gtacccggcg aacatggcca gagaccgacc
     1921 ccgccgcact cgtgccgact acgagcgcgg actgcccgga taccacgatc ccaccaccgg
     1981 gttcggcggc gcggcacccg cccgcagcgc cctgacgctg cgggccgtgc tcgcggtgtt
     2041 cgggctcgtc ttcgcggcgg gggcggcctg gttgggcttc gccgccgggc tcccggtact
     2101 cgggtgggtg atggtcgcgg tggccgtggt cgccatcggc gacctcgtgt gggtgctcta
     2161 ccggaagcgg cggggcgagc ccggctagga cacgcgctga ccgcttcggg agagcagcgt
     2221 ctcgtcgagg tggtggagga gctccgccgg ggagtcgaag acgcccaacg ccccggccgc
     2281 gcacagctcg tcgaccgaga aaccaccgct gcgcacggcg tagccggggg cgctgagtgc
     2341 ggctgcggcc cgaaagtccc acaccgagtc gccgatcatc acgcccgcgc tgccgtcgac
     2401 ggcacgcaag gccgcctcca gcaggtcggg ggcgggcttg gtgcgctgca cgtcctcgga
     2461 ggtggtccag gcgtcggcga gctccttgcc gtcgaacagg ccgaggtagt gctcgacgtg
     2521 ctc
//
LOCUS       DASRSC010000156        12142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2837529,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000156 DASRSC010000000
VERSION     DASRSC010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2837529"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VFE39_07545"
     CDS             complement(<1..423)
                     /locus_tag="VFE39_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258489.1"
                     /translation="MSFVDHLAGLDAARLTALLEFRPDVLVEPVPRGFGELALRLNGV
                     ESLSHALPLLNHDQVVTTRAIAMLGSATVPTLAGRLRAAEAEVRQVVDELCNRGLAWD
                     VHGRVGLPERLSEHLAAGLAAFRRLALIAQQARVDDLRV"
     gene            727..1380
                     /locus_tag="VFE39_07550"
     CDS             727..1380
                     /locus_tag="VFE39_07550"
                     /inference="COORDINATES: protein motif:HMM:NF018604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 family protein"
                     /protein_id="HET6258490.1"
                     /translation="MTAAPENVSMRWAGAVPAALAACAGAVDLLALAGLGGAFAGVIT
                     GNLVTAGYGIGTGDVELIAPTSVAVAGFTLGVLAWARLWRGRPRALVGPLLAELVLLI
                     VVAAGWAATRAHPGTAVSLALLALAAIAMGGQSVAALRLQASTTYMTGTLVTVLQDVV
                     TGRAGRRRQALRQLIALVAGAAAAAALLHTLPWAVPLLPIVLLTASVGLVAANRTSD"
     gene            1751..3193
                     /locus_tag="VFE39_07555"
     CDS             1751..3193
                     /locus_tag="VFE39_07555"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HET6258491.1"
                     /translation="MDGVAQGQNYIDGRWEDGDGPVLEVRDPATGEVAARVPDSTEQD
                     VDRAVVAARHAWSTWRWVNPTRRADYLHDAAEALTATQDRVAASITREMGKPLAEAVG
                     EVQKLAKTFHFYAEEATRITGATLANEEDGFTSLVEKEPIGVVAAITPWNYPVELIGW
                     KLAAALAAGCTVVVKPSEYTPSAAIEVFRCLEAARLPAGVANLVTGGGATGRALVAHP
                     AINKVAFTGSGATGEAIFRTVSGVKPLSMELGGNCPLIVTGHADLEQAVAGALRRSFR
                     NAGQICIAINRAYVHADLYEEFVQRLAKAAAGLVVGDGLREDKVDVGPVTNADILEKS
                     ERHVADARSKGARVLVGGGRSPDRPVGNFYQPTVLADCTQDMLVMHEETFGPVIGVSS
                     YDDLDTTIELANSTPAGLAAYVYSDDLSEVFALSRRLDFGSVAVNNVDAGTINAPYGG
                     RKGSGFGYEHGREGMEGYLQIKHVRLRHGS"
     gene            3255..4769
                     /gene="xylB"
                     /locus_tag="VFE39_07560"
     CDS             3255..4769
                     /gene="xylB"
                     /locus_tag="VFE39_07560"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HET6258492.1"
                     /translation="MTTCLIGIDLGTSACKVLALDASGQVLATSVREYPVLTSRPGWS
                     EQEPARWWQATDDAVQELLGRLPTGTAVRGFGLSGQMHGLTALDEERRVIRHAILWND
                     QRAAPQCEEITERAGGLPGLLDLVQNRMLPGFTAGKILWLRQHEPASFKDMRHLLNPK
                     DFLRLQMTGELATEVSDASGTGLFDVRRRRWCTELLQLLDIPEDLLPRVVESHEATGT
                     LLPDVADRWGLPASTPVFGGGGDAVIQTTSMGIIDSGSVGITLGTAGIVAGAGGACPD
                     DVDGTLQISCGNAPDRWHLMGVSLVAGGAFQWLRDALRPLVGSQLEYGALVELARQAP
                     PGSEGLLFLPYLLGERCPHVAPDARGSWVGLTPMHSAAHLSRSVLEGVLLNLREIVEV
                     CESTGLDCQEVRVSGGATVEPLWLQMLADVLQREVVTVTGAAEGGAYGAALLAGVGTG
                     DLGTLTDAAALVRETSRYAPDAGNAEVYARSFTIHRRLYDTLEPTFAELAGAEQ"
     gene            4808..5512
                     /gene="rpiA"
                     /locus_tag="VFE39_07565"
     CDS             4808..5512
                     /gene="rpiA"
                     /locus_tag="VFE39_07565"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET6258493.1"
                     /translation="MTQQDRAKVAAGVRALDTYVRDGMLLGLGSGTTSHWFVRSLAER
                     VREGLDVVGVPTSTATRDLALELGIRLADLNDVPELDLTIDGPDEIDGAGSMIKGGGA
                     CLLWEKVVARASRRMVTILDDTKIVSTLGAFPLPVEVIPFGWVSTRGHLARLFADAGL
                     GQVEITNRMRGNELLVTDSGHYILDAHLETIPDVPALAGVLNDIPGVVEHGLFVGIAD
                     AMVVGHPDGSADVRSL"
     gene            5543..5668
                     /locus_tag="VFE39_07570"
     CDS             5543..5668
                     /locus_tag="VFE39_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258494.1"
                     /translation="MTQSTDVPPEIVAQPRSVCLGLPEAYEEPAWVVRSGDGRSA"
     gene            5804..7225
                     /locus_tag="VFE39_07575"
     CDS             5804..7225
                     /locus_tag="VFE39_07575"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6258495.1"
                     /translation="MSVLVREPARPTQLRNWPHAWLLAVATVCLGAFMGQLDASIVTL
                     TYRPVGAEFGTSLAGVQWVSLSYLLTLAALLIPVGRLADTHGRKLLYLYGFVVFTVAS
                     VACGLAPTLGWLIAFRVVQGIGAAMLQANSVALITTSAPPSRMRAALGIQAAAQAVGL
                     ALGPTVGGLIVSTLGWRWVFGINVPIGVLAVVAGVFLLPRTHQRSTATTWDFTGLALL
                     LTATTAGLLGLSSLSGLALPGWVPPHLFWIAAAAVLGVLARQRWAAAPLLDPALLRSR
                     TVDTGLIGALGAYLILFGPLVLVPVVLTSAGSSELHAGLVLTALPAGFALAATAGERL
                     LPKTLTDRDRCLVGAVLIVVATGVLAVAPLTQAWLAPMLGALGIALGIFTPANNSLVM
                     RSIPIQAAGTGGGLVNMTRSLGTALGVALVTLALHLRPADATPLTGPRTALLSLTAVA
                     VILLLSAWLVRRPDRDPGCLPPR"
     gene            7536..8168
                     /locus_tag="VFE39_07580"
     CDS             7536..8168
                     /locus_tag="VFE39_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258496.1"
                     /translation="MPAPAPSPPGLHILPTGPGPEVLVRVPAAGAATLRLVVTLHGAG
                     GNARGGLAPLLPLADANGLLLLAPTSQGSTWDALTGRWGPDVRRIDDALAHVFESYPV
                     DQARLAISGFSDGASYALSLGLANADLFTHIIAFSPGYIAPARRVGTPRVYLSHGRED
                     TVLPIERTSRRIVPQLRRTHVPIEAHEFDGPHTVPTAVAEDAVRWLSEVP"
     gene            8257..9714
                     /locus_tag="VFE39_07585"
     CDS             8257..9714
                     /locus_tag="VFE39_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6258497.1"
                     /translation="MDHDDVPVLNALAEYHRHKHTPFTPPGHKQGRGADPRVRAVLGD
                     DVFRNDVLAISGLDDRSSSGGVIQRAQELMADAVGADQAFFSTCGSSLSVKSAMLAVA
                     GRHGRILVGRDVHKSVISGLVLSGLQPAWVHPRYDAELHLAHPPSPESIERALDREPD
                     VGGVLITSPTPYGTCADIAAIAQLCHGRGLPLLVDEAWGAHLPFHEQLPTWAMDAGAD
                     LCVVSIHKMGAGLEQGSVFHLQGDLIDPAHLSACADMLATTSPNVLVYAAMDGWRRQM
                     VQHGHDLLSDALDLAGDLRQRITKLPGLHVLHDELIGAEASHELDPLKVIIDVAGLGI
                     SGYQAADWLRAECAIDMGLSDHRRVEAQLTIGDDETTSALLLDALASLPEATGRLPEP
                     PPVSLPAPEDLELDTVMLPRDAFFARTEDVHAEDAAGRICAEQVTPYPPGIPVLLPGE
                     RIGQAALDYLRSGVEAGMVLPDPANPQLETIRVVA"
     gene            complement(9779..9970)
                     /locus_tag="VFE39_07590"
     CDS             complement(9779..9970)
                     /locus_tag="VFE39_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET6258498.1"
                     /translation="MKITTDRERCIGAGQCVLNAPGVFDQDDEGTVIVLDDHPSPEEE
                     PTVRAAEHACPARVISVVT"
     gene            complement(9981..11216)
                     /locus_tag="VFE39_07595"
     CDS             complement(9981..11216)
                     /locus_tag="VFE39_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6258499.1"
                     /translation="MTPTTVTAGNDAGASATPKYPMKRGCPFDPPAELAQLRAEKPIS
                     RVRLWDGSSPWLVTRYGDVRSLLADPRVSADIDRPGYPATSASILARRKNSRSFIAMD
                     DPEHARYRKMLISDFTVRRTESLRPLVEKIVNELLDAMERGPRPVDLVEAFALPLPSM
                     VICQMLGVPYADHDFFQSHSRALLDLSVDVQESMRANDELREYLTGLVAAKEAEPTDD
                     VLGRLAQQRVLTGELSRDDAVSMALLLLVAGHETTANMLGFGTLTLLQHPEQAAEVRG
                     GDPAVVNSTVEELLRLLTVVHSGRRRVAIEDIEIGGVTIHAGEGIIAAGEAANRDPAA
                     FVLPDDLDIHRANNHHVAFGFGVHQCLGQPLARLELQIAYPALLRRFPGLRVDVPVEE
                     IRFRDQMVVYGVQALPVNW"
     gene            11447..12118
                     /locus_tag="VFE39_07600"
     CDS             11447..12118
                     /locus_tag="VFE39_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6258500.1"
                     /translation="MDLGSPRVLLVEDDPELQAMLDALLTEDGYQVEVAADGHRGLHL
                     GLTRRYDVLLLDRRLPAVDGLDLLGRLRGRGVTTPVLILSALANPSDRVAGLDAGAED
                     YLGKPFDIDELLARLHALRRRHLDAARALPIGEGRLDLDTREVLAGDDRAVVRLSERE
                     CALLATLAGRPTRVYSRHDLLAIAFPEAESEVVVDTYVHYLRRKLGRGIVSTVRGRGY
                     QLGKL"
BASE COUNT         1722 a         4412 c         4179 g         1829 t
ORIGIN      
        1 gacccgcagg tcgtccaccc gtgcctgctg cgcgatgagg gcgaggcgcc ggaacgcggc
       61 gagcccggcg gcaagatgct cggagagccg ctcggggagc ccgacccgcc catgcacgtc
      121 ccacgccagc ccgcggttgc acagctcgtc gacgacctgc cgcacctcgg cctcagccgc
      181 acggagccgg cctgcgaggg tcgggaccgt tgccgacccg agcatcgcga tcgcccgggt
      241 ggtgaccacc tggtcgtggt tcagcagcgg gagggcatga gacagcgatt cgaccccgtt
      301 cagccggagc gcgagctccc cgaatccgcg cggcaccggc tcgaccagga catcgggccg
      361 gaactccagc agcgcggtca ggcgcgcggc gtcgagacca gcaaggtgat cgacgaacga
      421 cacgacgtca atcgcagcac gccgcccgcg agatcggtca gccgggtgcg gtgaccgcgt
      481 cgcgcccgct cgtgtcgatc gctctacacc agccaaccga gctggcaagg atcgtcaccg
      541 ggccggtcag catcgccttc agccggcgtg gtcagcgact gggcgaaggt cgggggcgct
      601 ggacgaaccg tcggtatggc cgactcccgc cacaacatca gcggtgtgaa tctgtcatcg
      661 gacgcagctc cttgggatcg atgcctgtgg taggagtcgg cgggtgccag cgcgcaggga
      721 gccgacatga ccgcagcccc ggaaaacgtg tcgatgcggt gggccggggc tgttccggct
      781 gcgctcgccg cctgcgcggg cgcggtcgac ctgctcgcgc tcgccgggct cggcggcgcg
      841 ttcgccggcg tcatcaccgg caacctggtg actgccgggt acggcatcgg cacgggcgac
      901 gtcgagctga tcgcgccgac gagcgtcgcc gtcgccggtt tcacactcgg ggtactggcc
      961 tgggcgaggt tgtggcgggg ccgtccgcgg gcgctcgtcg gcccgctgct cgccgagctg
     1021 gtgctcctga tcgtcgtggc cgcgggctgg gccgccacac gagcgcaccc cgggacggct
     1081 gtgtcgttgg ccctgctggc tctggccgcg atcgccatgg gtgggcagag cgtggccgcg
     1141 ttgcggctgc aggcctcgac gacgtacatg accgggacgc tcgtcaccgt gctgcaggac
     1201 gtcgtgaccg gccgggccgg acgccgccgc caggcactgc gtcagctcat cgcgctcgtg
     1261 gccggggccg ccgccgccgc ggcgctgctc cacaccctgc cctgggccgt gcccctgctg
     1321 ccgatcgtcc tgctcacggc gtccgtcggg ttggtggccg ccaaccgcac ctccgattga
     1381 gcagcgtggc ggcgcgctga ccggacatcg tgggatgcct tccgtggcga cccctcgcgc
     1441 gccacggaag gcatccaccc cgcgccccac gcgtaggcag tcggcccgtt gtgtctccca
     1501 cccccactca cgctgtgcag cggggaaagg tacagccagc cgtggcgaac tcccgacggc
     1561 cgcccccgca ccgtcgtgag ctgcggtgat gtcaccgaca agtgagccag atcgcatcgc
     1621 tgggacagcg tcatagtcga tggttgtccc gaatgaccgg gaccgcctcc cgtcgttcgg
     1681 gtcctccgct gccgacccgt aggtacggtt gcccgagcag gcccctcgtc gacggaggaa
     1741 ctgaagcggc atggatggcg tggcccaggg ccagaactac atcgacggtc gctgggagga
     1801 cggcgacggc ccggtactgg aggtgcgcga cccggcgacg ggagaggtcg ccgcccgggt
     1861 tccggactcc acggagcagg acgtcgaccg cgccgtcgtc gccgcccggc acgcgtggag
     1921 cacatggcgg tgggtcaacc cgacgcgcag ggcggactac ctccacgacg ccgccgaggc
     1981 cctcaccgcg acgcaggacc gggtggcggc gtcgatcacg cgggagatgg gcaagccgct
     2041 cgcggaggcc gtgggagagg tgcagaagct cgcgaagacc ttccacttct acgccgagga
     2101 ggccacccgg atcaccggcg ccaccctggc caacgaggag gacggcttca ccagcctggt
     2161 cgagaaggag ccgatcgggg tcgtcgcggc catcacgccc tggaactacc cggtggagct
     2221 catcggctgg aaactggcgg cggcgctcgc cgcgggctgc accgtcgtcg tgaaaccctc
     2281 ggagtacacc ccgtccgcag ccatcgaggt cttccgctgc ctggaggcgg cgcggctccc
     2341 cgcgggcgtg gcgaacctcg tcacgggcgg cggagcgacg gggcgggcgc tcgtggcgca
     2401 ccccgcgatc aacaaggtgg cgttcaccgg ttccggggcc accggcgagg cgatcttcag
     2461 gaccgtctcc ggggtcaagc cgctctccat ggagctgggc ggtaactgtc cgctcatcgt
     2521 gaccgggcac gcggacctgg agcaggcggt ggcgggcgcg ctacggcggt ccttccgcaa
     2581 cgccgggcag atctgcatcg ccatcaaccg cgcctatgtg cacgcggacc tctacgagga
     2641 gttcgtccag cggctcgcga aagcggcggc gggcctggtt gtgggcgacg gcctgcgcga
     2701 ggacaaggtc gatgtcgggc cggtcaccaa cgccgacatc ctcgagaagt ccgagcggca
     2761 cgtggcggac gcccggtcca agggcgcacg cgtcctcgtc ggaggcggcc gctcacccga
     2821 ccgtcccgtg ggcaacttct accagccgac cgtgctggcc gactgcaccc aggacatgct
     2881 cgtcatgcac gaggagacct tcggcccggt catcggcgtg agctcctacg acgacctcga
     2941 caccacgatc gagctggcca acagcacccc cgccgggctc gcggcctacg tctactccga
     3001 cgacctctcc gaggtgttcg cgctgagccg ccgcctcgac ttcggcagcg tcgcggtgaa
     3061 caacgtcgac gccggcacga tcaacgcccc ctacggcggg cgcaagggca gtggcttcgg
     3121 ctacgagcac ggccgggaag gcatggaggg ctacctccag atcaagcacg tgcggttacg
     3181 gcacggtagt tgacgcccgg cagcggtgcc gggcgcagcg gctcgacgac gagttgcggg
     3241 agagggagcc acacatgacc acgtgtctca tcggtatcga tctcggaacg tccgcctgca
     3301 aggtgctcgc cctcgacgcg agcgggcagg tgctcgccac cagcgtccgc gagtacccgg
     3361 tgctcacatc ccgccccggc tggtcggagc aggagccggc gcggtggtgg caggccaccg
     3421 acgacgcggt ccaggagctg ctgggccggc tgcccactgg taccgccgta cgcggtttcg
     3481 gtctgagcgg gcagatgcac ggcctcaccg ccttggacga ggaacgccgc gtcatccggc
     3541 acgccatcct gtggaacgac cagcgggcag cgccgcagtg cgaggagatc accgagcggg
     3601 ctggcgggtt gcccggcctg ctggacctgg tgcagaaccg catgctgccc ggcttcaccg
     3661 ccgggaagat tctctggctg cgccagcacg agcccgcctc cttcaaggac atgcgccacc
     3721 tgctcaaccc caaggacttc ctgcgcctgc agatgaccgg cgagctggcc acggaggtct
     3781 ccgacgcctc gggcaccggc ctgttcgacg tccgccggcg ccgctggtgc accgagctgc
     3841 tccagctgct cgacatcccg gaggacctgc tgccccgggt cgtggagtcg cacgaggcca
     3901 ccgggacgtt gctgccggac gtcgctgacc gttggggcct gccggcctcg accccggtgt
     3961 tcggtggtgg cggtgacgcc gtgatccaga cgacctcgat gggcatcatc gacagcggct
     4021 ccgtcgggat cacgctcggc acggccggca tcgtggcggg ggcaggaggg gcctgcccgg
     4081 acgacgtcga cggcaccctg cagatctcgt gcggcaacgc cccggaccgg tggcacctga
     4141 tgggcgtctc tctggtggcc ggcggagcgt tccagtggct gcgggacgcc ctgcgcccgc
     4201 tggtgggttc gcagctggag tacggcgctc tggtggagct ggcccggcag gcgccgcccg
     4261 ggtccgaggg cctgctcttc ctgccctatc tgctgggcga gcgctgcccg cacgtggctc
     4321 ccgacgcccg cggcagctgg gtgggcctca cgccgatgca ctcggccgcc cacctctccc
     4381 gcagtgtgct ggagggcgtc ctgctcaacc tgcgcgagat cgtggaggtg tgcgagtcca
     4441 ccggcctgga ctgccaggag gtgcgggtct ccgggggtgc caccgtggag ccgctgtggc
     4501 tgcagatgct ggccgacgtg ctgcaacgcg aggtcgtcac ggtgacggga gccgcggagg
     4561 gcggcgccta cggagctgcc ctgctcgccg gggtgggcac cggtgacctc ggcacgctca
     4621 ccgacgccgc agccctcgtc cgggagacct cccggtacgc ccccgacgcc ggcaacgccg
     4681 aggtctacgc gcggtcgttc accatccacc gtcgcctcta cgacaccctg gagcccacct
     4741 tcgccgagct cgccggcgcg gagcagtgaa cgcccctggc tcccggacgg cgaacgtggt
     4801 gcagccggtg acccagcagg accgggccaa ggtcgccgcc ggagtgcggg cactggacac
     4861 ctacgtccgg gacgggatgc tcctcggact cggttccggg acgacgtcgc actggttcgt
     4921 gcgctccctc gccgagcgcg tgcgggaggg cctggacgtg gtgggggtgc cgacctcgac
     4981 cgccaccagg gacctggcgc tggagctcgg gatccgcctc gcggacctga acgacgtccc
     5041 cgagctcgac ctcacgatcg acgggccgga cgagatcgac ggcgccggga gcatgatcaa
     5101 gggcgggggc gcctgcctgc tgtgggagaa ggtggtggcg agggcttctc gccgaatggt
     5161 caccatcctg gacgacacca agatcgtttc tacgctgggc gcgttcccgt tgccggtgga
     5221 ggtcatcccg ttcggctggg tctccacccg cgggcacctg gcccggctct tcgccgacgc
     5281 cggcctgggg caggtggaga tcacgaaccg gatgcggggg aacgagctgc tggtgaccga
     5341 cagcggccac tacatcctcg acgcgcacct ggagacgatc cctgacgtcc ccgcgctcgc
     5401 cggcgtcctc aacgacatac cgggggtcgt cgagcacggc ctcttcgtgg ggatcgccga
     5461 cgcgatggtg gtcggccatc cggacgggag cgcagacgtc cggtccctgt agccgggact
     5521 ccacttccgg ccgaaggggc gggtgaccca gtccacggac gtccctcccg agatcgtcgc
     5581 gcaaccccgg tcggtgtgcc tcggcctgcc ggaggcctac gaggagccgg cgtgggtggt
     5641 acgcagcggc gacggccgat cagcgtgatg acgtgagctc ctcggcaggg cgtgacgttc
     5701 tccacacctt ggaaccaccc ccagaatgta cggtcattac aggtgcccgt ccgatgggag
     5761 aggcgccgac agcgcggttg cgagcggggg agtcgccgtg tggatgtccg tcctcgtccg
     5821 ggaacctgcc cgcccgactc agttgcgcaa ctggccacat gcctggctgc tggccgtggc
     5881 gaccgtgtgc ctcggcgcct tcatgggcca gctcgatgcc agcatcgtca ccctcacgta
     5941 cagacctgtc ggcgccgagt tcggcacgtc cctggccggc gtccagtggg tctccctctc
     6001 ctacctcctg acccttgccg ccttgctgat cccggtcggc cgcctggccg acacccacgg
     6061 ccgcaagctg ctctacctct acgggttcgt ggtcttcacc gtcgcctccg ttgcctgcgg
     6121 gctggcaccg acgctcggct ggttgatcgc gttccgggtc gtgcagggca tcggcgcagc
     6181 catgctgcag gccaacagcg tcgcgctgat caccaccagc gctccaccga gccggatgcg
     6241 cgccgcactc ggcatccagg cagcggccca ggccgtcgga ctcgccctcg gcccgacggt
     6301 cggcggtttg atcgtctcca cgctgggctg gcggtgggtg ttcggcatca acgtcccgat
     6361 cggcgtgctc gccgtcgtcg caggagtctt cctcctcccc cgcacgcacc agcgcagcac
     6421 cgccacgacc tgggacttca ccggcctcgc gctgctcctc accgcgacca ccgcggggct
     6481 gctgggtctg tcgtcgctgt ccggcctggc gctaccgggc tgggtgccgc cgcatctgtt
     6541 ctggatcgcg gcagctgccg tactcggcgt cctcgcccgc cagcggtggg ccgcggcacc
     6601 gctgctcgac ccggccctcc tccgctcccg cacggtcgac accggcctga tcggcgccct
     6661 gggcgcctac ctgatcctct tcggaccgct cgtcctcgtc ccggtcgtgc tcacgtcagc
     6721 gggatcgtcc gagttgcatg ccggcctcgt actgacggct ctaccggccg gcttcgcact
     6781 ggcagccacg gccggcgaac ggctcctgcc gaagaccttg accgaccgcg atcgatgtct
     6841 cgtcggcgca gtgctcatcg tcgtcgcgac gggtgttctg gccgttgcac ctctcaccca
     6901 ggcgtggctc gcaccgatgc tcggcgccct cgggatcgcc ctgggcatct tcaccccggc
     6961 gaacaacagc ctcgtcatga ggtccatccc cattcaggct gccggtaccg gcggcgggct
     7021 ggtgaacatg acccgcagcc tcggcaccgc gctcggtgtc gccctggtga ccctcgccct
     7081 ccacctacgc ccggccgacg cgactccgct caccggtccg cgtaccgcgc tgctctcgct
     7141 cacggcagtc gccgtgatcc tcctgctctc cgcctggctc gtcaggcgtc cggatcgaga
     7201 tccgggttgc ttgccgccac gctgagcacg ccgtccaggt tgttgagcct cggcgagcat
     7261 gcggcggctc acgacgacgt gacgcatggt ctgctgggta ctcgtgaccc ggccgggccg
     7321 cccgggcccg gagaagcagg tactgacttc tggtgccgat ccctcgccgg acactcctgc
     7381 acgctgcggt tatggctgtg acgaccgccg cgacgagcgc tgcgctgggc gggtgcggcc
     7441 cggtgccggg agacttctcc gggatgaacc ctttctcacc gagcggagcc gactgcagga
     7501 gtgcccgtct caacgtccga gttcccactg cagccgtgcc cgctcctgct ccatcgccac
     7561 cgggcctgca catcctgccc accggaccgg gccccgaggt gctggtgcgc gtgcccgctg
     7621 cgggggccgc caccctgcgc ctggtggtga ccctgcacgg agccggcggg aacgcccggg
     7681 gcggcctcgc tcccctcctc ccactggccg acgccaacgg gctcctcctg ttggcaccga
     7741 cctcgcaggg cagcacgtgg gacgccctca ccggccgctg gggaccggac gtgcgtcgaa
     7801 tcgacgacgc actcgcccac gtgttcgagt cctaccccgt cgaccaagcc cggctcgcca
     7861 tcagtggctt ctccgacggc gcctcgtatg cgctctcgct gggactcgcc aatgccgacc
     7921 tgttcacgca catcatcgcg ttctcacctg gctatatcgc tcccgctcgc cgggtcggca
     7981 caccgagggt ctacctctcc cacggccgtg aagacaccgt gctccccatc gagcggacga
     8041 gccgccgcat cgtcccgcaa ctccggagaa cacacgtgcc catcgaggcg catgagttcg
     8101 acgggcctca cacggtgcct acggccgtcg ccgaggacgc ggtgcgctgg ctttccgaag
     8161 ttccctgacc tgccgaggtc ctcgacccgc cggtcgggcg tccccgcggt aaatggtgtg
     8221 tgtggggggc gacgtgcgac gggtacgaca gcatctatgg accacgacga cgtacccgtg
     8281 ctgaacgcgc tcgccgagta ccaccggcac aagcacacgc cgttcacacc gccgggccac
     8341 aagcagggcc gcggtgcgga tccacgggtg cgggcagtgc tgggcgatga tgtcttccgc
     8401 aacgacgtgc tcgccatctc cgggctcgac gatcggtcgt cgtcgggagg agtgatccag
     8461 cgggcccagg agctcatggc agatgccgtc ggagctgatc aggcgttctt ctccacgtgc
     8521 ggcagctcgc tctcggtcaa gagcgccatg ctcgcggtcg ccgggcgcca cggtcgcatc
     8581 ctggtcggac gcgacgtgca caagtcggtc atctccgggc tcgtcctctc cggcctgcag
     8641 cccgcctggg tgcacccgag gtacgacgcc gagctgcacc tcgcccatcc gccctcgccg
     8701 gagagcatcg agcgggctct cgaccgcgaa cccgacgtcg gcggcgtgct gatcaccagc
     8761 ccgacgccct acggcacctg cgccgacatc gctgccatcg cccagctctg ccacggtcgc
     8821 ggcctcccgc tcctcgtcga cgaggcctgg ggcgcgcacc tgccgttcca cgaacagcta
     8881 cccacctggg ccatggacgc cggcgccgac ctgtgcgtcg tgagcatcca caagatgggc
     8941 gccggcttgg agcagggttc ggtgttccac ctgcagggcg acctcatcga tccggcccac
     9001 ctcagcgcat gcgcggacat gctggccacc accagcccga acgtgctggt ctacgcggcg
     9061 atggacggct ggcgccgcca gatggtccag cacggccacg atctgctctc cgacgccctc
     9121 gatctcgcag gcgacctgcg gcagcgcatc acgaagctcc ctggcctgca cgtcctgcac
     9181 gacgagctga tcggcgccga ggcctcccac gaactggacc cgctgaaggt catcatcgac
     9241 gtggccggcc tggggatctc cggttaccag gcggcggact ggttgcgcgc cgaatgcgcg
     9301 atcgacatgg ggctctcgga ccaccgacgc gtcgaggcgc agctcacgat cggcgacgat
     9361 gaaaccacga gcgcactact cctggacgcg ctcgccagcc tgcccgaggc cacaggccgc
     9421 ctcccggaac cgccgccggt cagcctcccg gcgcccgagg acctcgagct cgacaccgtc
     9481 atgctgccga gggacgcgtt cttcgcccgc accgaagacg tgcatgccga ggatgccgca
     9541 ggccggatct gtgccgagca ggtcactccc tacccgcccg ggatccccgt cctgctcccc
     9601 ggcgaacgca tcggccaggc ggcgctggat tacctccgca gcggcgtgga ggccgggatg
     9661 gttctccccg atccggcgaa ccctcaactc gagaccatcc gggtcgtcgc ctgatccgat
     9721 cagccggtgg accgcggcca ccgggggcgc ccccacccga tcgagcgtcc tccgccggtc
     9781 acgtcaccac gctgatcacg cgcgccgggc aggcgtgctc agcggcccgg acggtgggct
     9841 cctcctcggg cgaggggtga tcgtcgagca cgatcaccgt cccctcgtcg tcctggtcga
     9901 acacgccggg agcgttgagc acgcactgac ccgcgccgat gcagcgttcc cgatcggtcg
     9961 tgatcttcat gtcgttcacc tcaccagttc accggcaatg cctgcacgcc gtagacgacc
    10021 atttggtccc ggaagcggat ctcttcgacc ggcacgtcga cccgaaggcc ggggaagcgg
    10081 cgcagcaacg ccgggtaggc gatctgcagc tccagccggg ccaggggttg cccgaggcac
    10141 tggtgtacgc cgaagccgaa cgcgacgtgg tggttgttgg cgcggtggat gtcgagatcg
    10201 tcgggtagga cgaacgccgc ggggtcacgg ttggccgcct cgcccgcggc gatgatgccc
    10261 tcgccggcgt gaatggtcac cccgccgatc tcgatgtcct cgatggcgac ccgacgccgt
    10321 ccggagtgca cgacggtgag cagccgcagc agctcctcca cggtgctgtt caccaccgca
    10381 gggtcaccgc cgcgcacctc tgcggcctgc tccgggtgct gcaacagcgt caacgtgccg
    10441 aagccgagca tgttcgccgt ggtctcgtga cccgcgacga gcagcaggag ggccatcgac
    10501 accgcgtcat cgcggctgag ctcgccggtc agcacgcgct gctgcgcgag ccggcccagc
    10561 acgtcatcgg tcggctcggc ctccttcgca gccacgaggc ccgtcaggta ctcgcgcagc
    10621 tcgtcgttgg cccgcatgga ctcctgcacg tcgaccgaca ggtcgagcag ggctcgggag
    10681 tggctctgga agaagtcgtg gtcagcgtac ggcacgccga gcatctgaca gatgaccatg
    10741 gaaggcagcg ggagcgcgaa cgcctcgacg aggtcgaccg gccgcggccc gcgctccatc
    10801 gcgtcgagga gctcgttcac gatcttctcg acgagcgggc gcagcgactc ggtgcggcgc
    10861 accgtgaagt cgctgatgag catcttgcgg taccgggcgt gctccgggtc gtccatcgcg
    10921 atgaagctcc gcgagttctt gcgccgggcg aggatcgacg ccgaggtggc cgggtagccg
    10981 ggccggtcga tgtcggcact gacccgcggg tcggcgagca ggctgcggac gtccccgtag
    11041 cgcgtgacca gccagggcga gctgccgtcc cagaggcgga cgcggctgat cggcttctcc
    11101 gcccgcagct gcgcgagctc ggcgggcggg tcgaaggggc agccgcgctt catcgggtac
    11161 ttcggcgtgg ccgacgcacc cgcgtcgttg ccggcggtga ctgtggtggg cgtcataaaa
    11221 cgacctcctc gttagcacga tgaagacatc ttagcaaagc taattacatt ctcgccactc
    11281 atgatcaaca ggggggcggc acgctcgtcc gcgaatcgcg cggttcgcga ggaaagccgg
    11341 aataggcgcc cctcttctgg gggttccagt tcctctcccc gccgtcctcc ggagcggcat
    11401 tcagggcatt cacagctcca ccgcgcagga tgggccagtg acgtcgatcg atctcggctc
    11461 cccgcgggtg ctcctcgtcg aggacgaccc ggaactgcag gccatgctcg atgcactgct
    11521 caccgaggac ggttaccagg tcgaggtcgc cgccgacggg caccgcggcc tgcacctggg
    11581 gctgaccagg cgttatgacg tcctcctgct ggaccgcagg ctgccggccg tcgatggcct
    11641 cgatctgctc ggcaggctac gcggccgcgg cgtgaccacg cctgtactga tcctctccgc
    11701 gctggccaac ccgtccgatc gggtggcggg cctcgacgcg ggcgccgagg actacctggg
    11761 caagccgttc gacatcgacg aactgctggc ccggctacac gccctgcgcc gtcgccacct
    11821 cgacgccgca cgggccctac cgatcggcga ggggcggctc gacctggaca cccgcgaggt
    11881 actcgccggc gacgacagag cggtcgtacg cctgtccgag cgggagtgcg cgctgctggc
    11941 cacgctggcc gggcgcccga cgcgggtcta cagccgtcac gacctgctcg cgatcgcgtt
    12001 cccggaggcc gagagcgagg tcgtggtcga cacctacgtg cactacctgc gccggaagct
    12061 tggtcgcggc atcgtctcga ccgtgcgcgg gcgcgggtac cagctgggca agctgtgact
    12121 ccgcgaggcg gccgaccccg cg
//
LOCUS       DASRSC010000157         2678 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2837810,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000157 DASRSC010000000
VERSION     DASRSC010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2837810"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VFE39_07605"
     CDS             <1..417
                     /locus_tag="VFE39_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258501.1"
                     /translation="LLGLGLAYNPVNGFAMTGTGIVIVLVAIYILMNVACIGYFARAA
                     NRAGRRWNVFLHLIVPVLGIVVFVPAWLTAAGIKVFSFVVPLSPPASYMGPGVAGFMV
                     LGIIYLGYLYAKHPRTVTEVGLVHLDEPASGVVTEG"
     gene            425..1621
                     /locus_tag="VFE39_07610"
     CDS             425..1621
                     /locus_tag="VFE39_07610"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HET6258502.1"
                     /translation="MSDLETITYRPGAGEYAWTFGGAAPVMRLRTPCVLDVYTEDAFA
                     GQVQSADDLVSRLNITGLNPQTGPFYIEGAEPGDTVAVHFVSLTPARDWAASTTVPLF
                     GALTGTHLTALLHEPLEEVMWIWQLDRDKQLCRFTAKKSDFTADLPMSPMHGTVGVAP
                     AGGEVRSALVPDAFGGNMDTPEMRAGTTCYLGVNVPGALLSLGDGHARQGEGETCGVA
                     VECAMNTVLIVDLIKGSGAPQTPWPRLENDEYIMSAGSARPLEDAFRIAQHDMVTWVA
                     ALCGLDPIDAYQFVTQAVESPLANVCDTNYTSIAKMPKRYLPATAYDGVHAHLRGLAA
                     SLLSPGGRPRLSVRGGPMAESADCIGPPRTDGVSPWFLFLQEVLRSLSAGYVLPGRYS
                     VEHQGS"
     gene            1706..>2678
                     /locus_tag="VFE39_07615"
     CDS             1706..>2678
                     /locus_tag="VFE39_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6258503.1"
                     /translation="MPDDVPLRLHLATMLLRAGLRDEAVRHLGAVLQREPGNAEAIRM
                     LTSPAEVAEPPASPPTDVDSSPERVRGSASPATGPSPVQDGPADDAPASRSDDSTDAY
                     DWTQAEDELRDVLPAMFVGEAGSVSAGLDEARAYDAEHTGLTLADVAGLDEVKKRLEA
                     AFLAPMRNPELRKLYGKSLRGGLLLYGPPGCGKTFIARAVAGELGARFITVSFADLID
                     MFVGRSERNIHELFEVARRNAPCVVFLDEVDAIGQKRSQLRNTPMRSAVNQLLLELDD
                     VASDNTGVFLLAATNHPWDVDSALRRPGRFDRTLLVLPPDGPAREGVF"
BASE COUNT          395 a          935 c          922 g          426 t
ORIGIN      
        1 ctgctcggcc tcgggctcgc gtacaacccg gtgaacgggt tcgcgatgac cggcaccggc
       61 atcgtgatcg tgctcgtggc gatctacatc ctgatgaacg tcgcgtgcat cgggtacttc
      121 gcgcgcgccg ccaaccgggc cggccgccgg tggaacgtgt tcctgcacct catcgtgccg
      181 gtgcttggca tcgtggtctt cgtgcccgcc tggctgaccg ccgctggcat caaggtgttc
      241 tccttcgtgg tgccgctcag cccgcccgcg tcgtacatgg ggccgggtgt cgcgggcttc
      301 atggtgctgg gcatcatcta cctcgggtac ctgtacgcga agcacccgcg gacggtcacc
      361 gaggtcggac tcgtccacct cgacgagccg gcctcgggcg tggtgacgga aggctgagct
      421 gacaatgagc gaccttgaga cgatcacgta ccggccaggg gccggcgagt acgcctggac
      481 cttcggcggc gccgccccgg tcatgcggct gcgcaccccg tgcgtgctcg acgtgtacac
      541 agaagacgcc ttcgcagggc aggttcagtc cgccgacgac ctggtcagca ggctgaacat
      601 caccgggctc aacccgcaga ccggtccctt ctatatcgag ggggccgagc ccggcgacac
      661 ggtggcggtg cacttcgtat cgctgacccc tgcgcgggac tgggcggcgt cgaccacggt
      721 gccgctgttc ggcgcgctga ccgggacgca cctgaccgca ctgctgcacg agccgctcga
      781 agaggtcatg tggatctggc agctcgaccg ggacaagcag ctgtgccggt tcaccgcgaa
      841 gaagtcggac ttcaccgccg acctgccgat gtcgcccatg cacgggaccg tcggcgtcgc
      901 ccccgccggg ggcgaggtgc gctccgcgct cgtgcccgac gcgttcggcg ggaacatgga
      961 cacccccgag atgcgcgcgg gaaccacctg ctacctgggc gtcaacgtgc cgggtgcgct
     1021 gctttcgctc ggcgacgggc acgcgcgcca gggcgagggc gagacctgcg gggtcgcggt
     1081 cgagtgcgcg atgaacacgg tgctcatcgt cgacctgatc aagggctccg gggcgccgca
     1141 gacgccgtgg ccgcggctcg agaacgacga gtacatcatg tcggccgggt ccgcgcggcc
     1201 gcttgaagac gcgttccgca tcgcgcagca tgacatggtg acctgggtgg cggcgctgtg
     1261 cgggctcgac ccgatcgacg cctaccagtt cgtcacgcag gccgtggagt cgccgctcgc
     1321 caacgtgtgt gacacgaact acacgtcgat cgcgaagatg cccaagcgct acctgcccgc
     1381 gaccgcctac gacggggtgc acgcccacct caggggactg gccgcgtcgc tgttatcccc
     1441 cgggggacgc ccccggctgt ccgtgcgcgg gggcccgatg gcggagagcg ccgattgtat
     1501 cgggcccccg cgcacggacg gggttagccc gtggttcctc ttcttacaag aggtgctgcg
     1561 gtccctctcg gctgggtatg tgttgccggg acggtactca gtagagcacc agggatcata
     1621 gtgttgaagg atcgcactcg actcccggaa cggacgcatg gaacctgaac cgctactgga
     1681 cagcctgcgt aacgcggtta acgcaatgcc ggacgacgtg ccgcttcgtc tccacctcgc
     1741 gacgatgctg ctgcgcgccg gactgcgtga cgaggcggtg cggcatctgg gtgccgtgct
     1801 gcagcgcgaa ccgggcaatg ccgaggcgat aagaatgctc acctccccgg ctgaggtcgc
     1861 cgaaccgccc gcgtccccgc cgaccgacgt cgatagttct cccgaacggg ttcgcggctc
     1921 cgcctccccc gccaccggtc cttcccccgt gcaggacggc ccggccgacg atgccccggc
     1981 gagcaggagc gacgactcga ccgacgccta cgactggaca caggccgaag acgagttgcg
     2041 tgacgtgctg cccgcgatgt tcgtcggcga ggcgggttct gtgtccgccg gccttgacga
     2101 ggcccgcgcc tacgacgccg agcatacggg gctcacgctg gccgacgtgg ccgggctcga
     2161 cgaggtcaag aagcggctcg aggccgcgtt cctcgcgccg atgcgcaacc ccgagcttcg
     2221 caagctttac gggaagtcac tgcgcggcgg gctgctgctc tacgggccgc ccggctgcgg
     2281 gaagacgttc atcgcccgcg cggtggccgg ggaactcggc gcgcggttca tcaccgtgtc
     2341 gttcgctgac ctgatcgaca tgttcgtcgg ccggtccgag cggaacatcc acgagctctt
     2401 cgaggtcgcc cggcggaacg ccccgtgcgt ggtgttcctc gacgaggtcg acgccatcgg
     2461 gcagaagcgg tcccagctgc gcaacacgcc gatgcgctcg gcggtcaacc agctgctgct
     2521 cgagctcgac gacgtggcgt ccgacaacac cggggtgttc ctgctcgcgg cgaccaacca
     2581 cccgtgggat gtcgactccg ccttgcgccg cccgggacgc ttcgaccgca cgctgctcgt
     2641 cctgccaccc gacgggcccg cccgcgaggg cgtgttcc
//
LOCUS       DASRSC010000158         8990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3655077,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000158 DASRSC010000000
VERSION     DASRSC010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8990
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3655077"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..563
                     /gene="ftsY"
                     /locus_tag="VFE39_07620"
     CDS             <1..563
                     /gene="ftsY"
                     /locus_tag="VFE39_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET6258504.1"
                     /translation="TTTTGKLARVLVAGGRHVVLGAADTFRAAAAEQLGTWGERAGAT
                     VVRGPEGADPASVAFDAVKQGSDEGADAVLLDTAGRLHTKTGLMDELGKVKRVVSRQA
                     AVDEVLLVLDATTGQNGLTQARVFGDVVDVTGIVLTKLDGTAKGGIVFRVQRDLGVPV
                     KLVGLGEGPDDLAPFEPAAFVDALLA"
     gene            724..2100
                     /locus_tag="VFE39_07625"
     CDS             724..2100
                     /locus_tag="VFE39_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HET6258505.1"
                     /translation="MLDTGDTAWMLASSALVLLMTPGLALFYGGMVRSKTVLNMMMMS
                     ISALGLVGVLWVLYGYSVAFGNDVGGGLLGNPAQFAGLHGLFGGTYLATQGGPPVDIP
                     LVGTIPATVFVAFQAMFAVITVALISGAVADRLRFGPWLLFAGLWATIVYFPVAHWVF
                     AFNGVTAATGGWIANNLKVIDFAGGTAVHINAGAAGLALALVLGKRRGWPREPMRPHN
                     LTLVMIGAGLLWFGWFGFNAGSAVASNGVAGITFVNTFVATGAAMLAWLLTERIRDGK
                     PTSLGAASGLVAGLVAITPSCSSVSPIGAIAVGAIAGVLCALAVGLKFRFGYDDSLDV
                     VGVHLVGGLAGTLLVGFFATTAAPAGVNGLFYGGGVDQLWRQAVGALAVLVFSFVVSL
                     ILAFIVKAIMGLRVSDEEEATGVDETEHAETAYDFSTLHGIGGIGTPRPSAATNAAAE
                     HVTAGKES"
     gene            2104..2442
                     /locus_tag="VFE39_07630"
     CDS             2104..2442
                     /locus_tag="VFE39_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET6258506.1"
                     /translation="MKLVTAIVKPFVLEDVKGALEQIGVLGMTVSEVQGYGRQKGHTE
                     VYRGAEYSVDFVPKVRVEVVADDTLADKVVDAIVEAARTGKIGDGKVWVIPVESVIRV
                     RTGERGTDAI"
     gene            2546..2884
                     /locus_tag="VFE39_07635"
     CDS             2546..2884
                     /locus_tag="VFE39_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HET6258507.1"
                     /translation="MMLVTAIIKPFALEPVRAGLERLDVAGMTISASSGYGRQRGHTE
                     FYRGGDYHVDFVPKVRVEVVVEDVAVEKVIEAVSVAARTGKIGDGKLWVTPVNTVVRV
                     RTGERGADAL"
     gene            2881..5235
                     /locus_tag="VFE39_07640"
     CDS             2881..5235
                     /locus_tag="VFE39_07640"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HET6258508.1"
                     /translation="MSAVAVASGDAGDLVRAKAVLLAPGAGKRRLGPEALRTALVDLH
                     DFWLSSRAAAIGLTDRAALVAVGALGRRELAPWSDLDLVLLHDGRKDVERLAEQLWYP
                     LWNAGIGLDHSVRTPGQAVQVAATDLRAAFGLLEARHIAGDGALSDKVRAAVRQAWRA
                     GIRGRFDELADSAQERWRKVGDVAHRVEPDLKNGHGGLRDVQLLDALAAAQLVDRPAG
                     DVVEARSLLLDVRTELHRLAGRARDVLRAQDADEVAATLEIADRFELARALSGAARSV
                     AFAAEVGLRSARSALPRRGLAALRRAPVRRPLDSGVVEHAGEVALARDAAAPRDPALV
                     LRVAATAARTGLPIAAGTLHRLADTAPELREPWPRPALGELLSLLGTGRPIVDVVESL
                     DRTGLWGRLFPEWGAVRDLPPRDRAHVWTVDRHLVEACAQAARLTTRVARPDLLLVGT
                     LIHDIGKGRAGDHSEVGESLAVQVGRRLGFAEPDVAVLGQVVRHHLLLPHTATRRDLD
                     DPATIMRVVETLDGNGLVLDLLEALAEADALATGPGVWSPWKQTLIRDLVGRCRTLLA
                     GDPLPAPAALDAAAADLATAVATDGQPQVRFDDRSDPATVIVAVPDRPGALAAAAGVL
                     ALHSLEVHAAELPSAEHAAVFTFTVSPRFGGLPDPALLRSDLIRVLDGGLTLADALAR
                     KERDYAPIIPSGEPPAPPRVLWFDDEATGAVVLELRGTDRIGLLHRVAAALGECGADL
                     RWARVATLGASVVDSFCVAGPGAGGALSATERRRIERAVLAAAR"
     gene            5313..6011
                     /locus_tag="VFE39_07645"
     CDS             5313..6011
                     /locus_tag="VFE39_07645"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6258509.1"
                     /translation="MTRATSTAALAATAGVLPVHIALPGRTLLLVAGMPGAGKSTLLG
                     GLPGGPGLAVFDSEIHRSALRRALGRLPYAWYRPLVHLLHRLAVLAAAVSSTPTVVVH
                     LPATDDRTRAVVARLAAATGRTAHLLWLHVDAADARQGQCERGRVVPETSFAGHAERA
                     ASTTAELLEGPPPGWSSVTVLDRASARGGLQLDTAPAGTSRTTSDGRRLTVVNGTSAR
                     HDDAPVERASMRPK"
     gene            6022..7563
                     /gene="ffh"
                     /locus_tag="VFE39_07650"
     CDS             6022..7563
                     /gene="ffh"
                     /locus_tag="VFE39_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET6258510.1"
                     /translation="MFDTLSERLSSTLANLRGKGRLSDADIDATAREIRIALLEADVA
                     LPVVRGFIARIKERAKGAEVSGALNPAQQVVKIVDDELVAILGGETRRLRLAKEPPSV
                     IMLAGLQGSGKTTLAGKLAHWLKGQGHAPLLVACDLQRPNAVTQLQIVGDRAGVTTFA
                     PEPGNGVGDPVDVARRGVAHARDKMFDVVIVDTAGRLGVDEELMRQAADIRDATRPDE
                     TLFVVDAMIGQDAVATAEAFRDGVGFTGVVLTKLDGDARGGAALSVREVTGEPILFAS
                     SGEKLADFDVFHPDRMASRILGMGDLLTLIEQAETVFDEEQTAKAAAKISSGELSLED
                     FLEQMLAIRKMGPIGNILGMLPGANAGQMKDALAQVDDKQLDKLQAIIRGMTPAERAD
                     PKIINGSRRLRIANGSGVQVSDVNELVNRFFEARKMMKQMAGQFGFGGGRSATRKQAK
                     GRKGKKGKGRGPSPARAGALPAGLGTGTPDLSGLPPSLRQLPPGLDQLPPGFDPSKLK
                     FPKGK"
     gene            7567..8658
                     /locus_tag="VFE39_07655"
     CDS             7567..8658
                     /locus_tag="VFE39_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6258511.1"
                     /translation="MYRLRGVLLGAGGQDGDRTVELFVDAAGMLVDEIPGAETIIDGG
                     FIVPGLVDAHCHIGLGASGAVDLEEADAQARVDRDAGTLLIRDCGSPLDTTPLQIRQD
                     LPEIIRAGVHVARPKRYIPGVAAELDDPWLLPEVVAEQAAAGDGWVKLVGDWIDRGIG
                     DLAPLWPDDVLVAAIDAAHAAGARVTAHVFGADALPGLIRAGIDCIEHGPGLTDDLIT
                     EMARRGTALVPTLINIETFPGIADAATKYPTYAAHMRELHAGTAGVVRRAVEAGVPVF
                     AGTDAGGGIVHGRIADEIIALHSAGLSAADALGAASWSARDWLGRPGLHLGARADLVV
                     YRSDPRADLSVLREPALVMLRGRPVHATG"
     gene            complement(8756..>8990)
                     /locus_tag="VFE39_07660"
     CDS             complement(8756..>8990)
                     /locus_tag="VFE39_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258512.1"
                     /translation="PLVSGGVLSGGLLWGRLVSGRLPPGGLLSGRSLSLAALSLAALS
                     LRSRERSSSGPSAAPTLPTLPAVAIGRRAQARA"
BASE COUNT         1235 a         3187 c         3235 g         1333 t
ORIGIN      
        1 agaccaccac caccggaaag ctggccaggg tgctggtggc gggtgggcgg cacgtagtgc
       61 tcggtgcggc cgacaccttc cgcgccgccg ccgccgaaca gctcggcacc tggggcgaac
      121 gcgcgggggc caccgtcgtg cgcggcccgg agggagccga cccggccagc gtcgccttcg
      181 acgccgtcaa gcagggctcc gacgagggcg ccgacgccgt gctgctcgac accgcgggcc
      241 ggttgcacac caagaccggt ctgatggacg agctgggcaa ggtgaaaagg gtcgtgtccc
      301 ggcaggccgc ggtggacgag gtactgctcg tcctcgacgc caccaccggc cagaacgggc
      361 tcacccaggc ccgcgtgttc ggtgacgtcg tggacgtcac cggcatcgtg ctcaccaagc
      421 tcgacggcac cgcgaagggc ggaatcgtct tccgggtgca gcgcgacctg ggcgtgccgg
      481 tcaagctcgt cgggctcggt gaggggccgg atgacctggc tccgttcgag cccgcggcct
      541 ttgtcgacgc tctgttggcc taatcatccg gctccgtaat ccggatgtaa ctccgcgagc
      601 tatcgcgttc accccctcga aacagcggcg gacgccgtcc gaaacatggc gatccgatag
      661 ttcccgcacc tgccgctcca cacgccatct ccaggcaagc ggcgaaaacg ggagggagtt
      721 gacgtgctcg ataccggcga taccgcctgg atgctggcca gctcagcgct ggtgctgctg
      781 atgacacccg gattggcgct gttctacggc ggcatggtcc gctcgaagac cgtgctgaac
      841 atgatgatga tgagcatctc ggcgctcgga ctggtcggcg tgctctgggt gctttacggc
      901 tactccgtcg cgttcggcaa cgacgtcggc ggcgggttgc tcggcaaccc ggcgcagttc
      961 gccggtctcc acggtctgtt cggcggcacc tatctcgcca cccagggtgg ccccccggtc
     1021 gacattccgc tggtcggcac gatccccgca actgtgttcg tggccttcca ggcgatgttc
     1081 gcggtcatca ccgtcgctct gatctccggt gccgtcgccg accgtctgcg gttcggtccg
     1141 tggctgctgt tcgccggtct gtgggccacc atcgtctact tcccggtcgc gcactgggtc
     1201 ttcgccttca atggcgtcac cgccgcgacc ggtggctgga tcgccaacaa cctcaaggtg
     1261 atcgacttcg caggcggcac cgcggtgcac atcaacgccg gtgcggcagg gctcgcgctc
     1321 gcgctggtgc tcggcaagcg ccgcggctgg ccgcgtgagc ccatgcgccc gcacaacctc
     1381 acgctggtca tgatcggagc cggcctcctg tggttcggct ggttcggctt caacgccggt
     1441 tcggccgtcg cctccaacgg cgtcgcgggc atcacgttcg tcaacacctt cgtggccact
     1501 ggggccgcca tgctggcctg gctactcacc gagcgcatcc gggacggcaa gcccaccagc
     1561 ctcggtgcgg cgtccggtct ggtcgccggt ctcgtcgcca tcaccccctc ctgttcgtcg
     1621 gtctcgccga tcggtgctat cgcggtcggt gcgatcgccg gtgtcctgtg tgccctggcc
     1681 gtgggcctca agttccgctt cgggtacgac gactcactcg acgtggtcgg tgtccacctc
     1741 gtcggcggtc tggccggcac cctgctggtc ggcttcttcg ccaccaccgc tgccccggcc
     1801 ggtgtcaacg gcctgttcta cggcggcggc gtggaccagc tctggcgcca ggccgtcggt
     1861 gcgctcgcgg tgctcgtgtt ctcgttcgtc gtgagcctga tcctggcctt catcgtcaag
     1921 gcgatcatgg gcctacgggt cagcgacgag gaggaggcta ccggcgtcga cgagacggag
     1981 cacgccgaga cggcctacga cttctccact ctccacggca tcggcggcat cgggacgcct
     2041 cggccgtccg cggccacgaa cgcggccgcg gagcacgtca ccgcaggtaa ggagagctga
     2101 gcgatgaagt tggtcacggc aatcgtcaag ccgttcgtcc tcgaggacgt caagggggcg
     2161 ctcgagcaga tcggcgtcct cgggatgacg gtcagcgagg tgcaggggta cgggcggcag
     2221 aagggccaca ccgaggtcta ccgcggggcg gagtactcgg tggacttcgt gccgaaggtc
     2281 cgggtggagg tcgtcgccga cgacaccctc gcggacaagg tggtggatgc catcgtcgag
     2341 gcggcccgca ccggcaagat cggtgacggc aaggtctggg tcattccggt cgagagtgtc
     2401 atccgggtcc gcaccggcga gcgcggcacc gacgcgatct gaccgcgtcg tcggctccga
     2461 gggcctgctg cacagcatga ccgtgcagcg ggcccttggc gcttgttccc gaagtgtccg
     2521 gttcatcact gatcggagga tcgcgatgat gctcgtgacc gccatcatca aaccgttcgc
     2581 gctggaaccc gtccgtgccg ggctcgagcg tctggacgtc gccggtatga ccatcagcgc
     2641 gtcgtccgga tacggccgcc agcggggcca caccgagttc taccgcggtg gggattacca
     2701 cgtcgacttc gtgccgaagg tgcgcgtcga agtggtagtc gaggacgttg ccgtggagaa
     2761 ggtgatcgag gcggtgtcgg tggccgcccg gaccgggaag atcggtgacg ggaagctgtg
     2821 ggtcacgccg gtgaacaccg tggtgcgggt ccgcaccggg gaacggggtg ccgacgcgct
     2881 gtgagtgcag tggcagtggc gtcgggggac gcaggggatc tggtccgggc gaaggcagtg
     2941 ctcctcgccc cgggcgcagg gaaacgcagg ctgggacccg aggcgttgcg taccgccctg
     3001 gtggacctgc atgacttctg gctgtcgtcg agggcggcgg ccatcgggct gaccgaccgc
     3061 gcggcgctcg tcgcggtcgg ggcgctgggt cgccgcgaac tggcaccgtg gtcggacctc
     3121 gacctggtgc tgctgcacga cggccgcaag gacgtcgaac ggctggccga gcagctgtgg
     3181 tatccgctgt ggaacgccgg gatcggcctc gaccactcgg tgcgcacacc gggccaggcc
     3241 gtccaggtgg ccgccaccga cctgcgcgcc gcgttcggcc tactggaggc ccgccacatc
     3301 gccggcgacg gtgcgctctc ggacaaggtg cgtgcggcag tgcggcaggc gtggcgtgcc
     3361 ggtatccggg gccggttcga cgagctcgcc gactccgccc aggagcgctg gcggaaggtc
     3421 ggcgacgtcg cacaccgggt ggagccggat ctgaagaacg ggcatggcgg gctgcgggat
     3481 gtccagctcc tcgacgcact cgcggcagcg cagctcgtgg atcggccggc cggggacgtc
     3541 gtggaggccc gcagtctgct gctggacgtc cgcaccgaac tgcaccggct cgccggtcgg
     3601 gcccgggacg ttctgcgcgc gcaggacgcc gacgaggtgg cagccacgct ggagatcgcc
     3661 gaccggttcg agctcgcgcg cgccctgtcc ggcgcagccc ggtcggtggc gttcgccgcc
     3721 gaggtcggcc tgcgttccgc gcggagtgcc ctgcctcgcc gtggtctggc cgcactgcgg
     3781 cgcgcccccg tgcggcggcc actggacagc ggtgtcgtcg agcacgccgg cgaggtggcg
     3841 ctcgcgcgag acgctgcagc gccgcgcgat cccgcgctgg tgctgagggt cgcggccacc
     3901 gcggcccgca ccgggctgcc gatcgccgcc ggcacgttgc acaggcttgc cgatactgcc
     3961 cctgagctgc gcgaaccctg gccacggcca gcactgggcg agctgctgtc gctgctgggc
     4021 accggacgcc cgatcgtcga cgtcgtggag tcactggacc gcaccgggct gtggggccgg
     4081 ctcttccccg aatggggcgc ggtgcgcgac ctgccaccgc gtgaccgcgc gcacgtgtgg
     4141 accgtcgacc ggcatctggt cgaggcctgt gcgcaggccg ctcggctcac cacccgcgtc
     4201 gcgcgtccgg acctgctgct cgtgggcacc ctgatccacg acatcggcaa gggccgcgcc
     4261 ggcgaccact ccgaggtcgg cgagtccctg gccgtgcagg tcgggcgacg cctcgggttc
     4321 gccgagcccg atgttgccgt gctggggcag gtggtgcgtc accacctgct gctgccgcac
     4381 accgctaccc gccgtgacct ggacgacccg gcgacgatca tgcgtgtcgt cgagaccctg
     4441 gacggcaacg ggctcgtcct cgacctgctc gaggccctcg cggaggccga cgcgctcgcc
     4501 accggtcccg gtgtctggag cccgtggaag cagaccctga tccgggacct ggtcggacgc
     4561 tgccggacgc tgctggccgg tgatcctctc cctgctccgg ctgcgctcga tgcggcggcg
     4621 gcggatctcg cgacagccgt cgccaccgac ggccagccgc aggtccggtt cgacgaccgc
     4681 agcgacccgg ccaccgtcat cgtcgcggtc ccggaccggc ccggggccct ggccgccgcc
     4741 gcgggcgtgc tggcgctgca ctccctggag gtgcacgcgg ctgagctccc gtccgccgag
     4801 cacgcggcgg tcttcacgtt caccgtctcg cctcgcttcg gcggcttgcc cgacccggcg
     4861 ctgctgaggt cggacctgat ccgggtgctc gacggtggcc tcaccctggc cgacgcgctg
     4921 gcccgcaagg aacgcgacta cgcccccatc atcccgagcg gagaaccgcc tgcgcccccg
     4981 cgggtgctgt ggttcgacga cgaggccacc ggggcggtgg tgctggagct gcgcggcacc
     5041 gaccggatcg gtttgctgca ccgggtggca gccgcgctcg gggagtgcgg tgccgacctg
     5101 cgctgggcga gggtggccac gctgggcgcg tctgtcgtcg actcgttctg tgtcgcagga
     5161 ccgggcgccg ggggagcgct gagtgccacg gaacggcggc ggatcgagcg ggcggtgctc
     5221 gctgccgcgc gctagctcct cagcacgccg catcgcggtg caccgggaga tcggaaagga
     5281 acgaacggca cggaacggac gttgagtcct gtgtgacgag agcgacatcg accgccgccc
     5341 tggccgccac cgcgggtgtc ctgcctgtgc acattgccct gcccggccgg acgttgctgc
     5401 tcgtggcggg tatgcccggc gcgggaaagt cgaccctgct cggggggctc ccgggcggtc
     5461 ctggtctcgc cgtgttcgac tcggagatcc accggtcggc gctgcggcgc gctctgggcc
     5521 ggttgcccta cgcgtggtac cgaccactcg tgcacctgtt gcaccggctg gccgtgctgg
     5581 ccgcagcggt ctcgtccacg cccacggtgg tcgttcatct gcctgccacc gacgaccgca
     5641 cccgtgcggt cgtcgcccgg ctcgctgctg ccaccggccg caccgcgcac ctgctctggt
     5701 tgcacgtcga cgcggccgac gcccggcagg gacagtgcga gcgtggtcgg gtggtgcccg
     5761 agacgtcctt cgccggacac gccgaacggg ccgcgtccac tacggccgag ctgctcgaag
     5821 gccccccgcc gggctggtcg agcgtcaccg tgctcgaccg cgcttcggcc cgcggtggcc
     5881 tgcagctgga caccgccccc gccggcacga gtcggaccac ctccgacgga cggcgtctca
     5941 cggtcgtcaa cggcacctcg gcacggcacg atgacgctcc ggtggagcgc gcgtcgatgc
     6001 ggcccaagta ggcttggtcg ggtgttcgac accctctccg agcgcctcag cagcaccctc
     6061 gccaacctgc gtggcaaggg gcggctttcc gacgccgaca tcgacgccac cgcacgcgag
     6121 atccgcatcg cgctgctgga ggccgacgtc gcgctgccgg tggtccgcgg cttcatcgcg
     6181 cggatcaagg agcgggccaa gggcgcggag gtctccggcg cgctgaaccc cgcgcagcag
     6241 gtcgtcaaga tcgtcgacga cgagctcgtg gcgattctcg gcggtgagac ccggcgtctc
     6301 cggctggcca aggagccacc gagcgtgatc atgctcgcgg gcctgcaggg ttccgggaag
     6361 accaccctgg cgggcaagct cgcgcactgg ctcaagggcc aggggcatgc accgctgctc
     6421 gtggcatgcg acctgcaacg gcccaacgcc gtcacccagc tgcagatcgt cggcgatcgc
     6481 gccggcgtca cgacgttcgc cccggagccc gggaacggcg tgggggatcc ggtggacgtc
     6541 gcccgtcgcg gcgtcgcgca tgcccgcgac aagatgttcg acgtcgtcat cgtcgacacc
     6601 gcgggccggc tcggcgtcga cgaggagctg atgcgccagg ccgccgacat ccgcgacgcc
     6661 acccggccgg acgagacgct gttcgtcgtc gacgcgatga tcggtcagga cgccgtggcg
     6721 acggcggagg cgttccgcga cggggtcggc ttcaccggcg ttgtgctcac caagctcgac
     6781 ggggacgccc gtggtggcgc ggcgctgtcg gtccgtgagg tgaccggcga gcccatcctg
     6841 ttcgcgtcca gcggtgagaa gctcgccgac ttcgatgtct tccaccccga ccggatggcc
     6901 agccgcatcc tcgggatggg cgacctgctc acgctgatcg agcaggccga gaccgtcttc
     6961 gacgaggagc agacggccaa ggccgcggcc aagatcagct cgggggagct gtcgctcgaa
     7021 gacttcctcg agcagatgct cgccatccgc aagatgggtc cgatcggcaa catcctcggc
     7081 atgctccccg gcgccaacgc ggggcagatg aaggacgcgc tcgcccaggt cgacgacaaa
     7141 caactggaca agctgcaggc catcatccgg ggtatgacgc cggcggagcg cgccgatccg
     7201 aagatcatca acgggtcccg gcgactgcgc atcgccaacg gctccggcgt gcaggtcagc
     7261 gacgtcaacg agctggtcaa ccgcttcttc gaggcgcgca agatgatgaa gcagatggcg
     7321 gggcagttcg gcttcggcgg tggccgcagc gctacccgga agcaggccaa gggacgcaag
     7381 ggcaagaagg gcaagggacg cggcccctca ccggcccgcg cgggtgcgct gccggcaggg
     7441 ctcggcaccg gtacccctga tctctccggc ctgccgccgt cgttgcgcca gctcccgccg
     7501 ggcctcgacc agctgcctcc tggcttcgac ccgagcaagc tgaagttccc gaaggggaag
     7561 tagcgcgtgt accggctgcg tggggtgctg ctcggggcag gtgggcagga cggagaccgg
     7621 acggtcgagc tgttcgtcga tgcagcgggc atgctggtgg acgagattcc gggcgccgag
     7681 acgatcatcg acggcggctt catcgttccg ggcctcgtcg acgcgcactg ccacatcggg
     7741 ctcggggcgt ccggcgccgt cgacctcgag gaggccgatg cccaggctcg cgtcgatcgc
     7801 gacgccggca ccctgctgat ccgcgactgc ggctcaccgc tcgacaccac cccgctgcag
     7861 atccgccagg atctcccgga gatcatccgc gccggcgtgc acgtcgcacg gcccaagcgc
     7921 tacatccccg gggtcgccgc cgagctcgac gatccgtggc tcctgcccga ggtcgtcgcc
     7981 gagcaggccg cggccggtga cggctgggtg aagctggtag gtgactggat cgaccggggc
     8041 atcggcgacc tggccccact gtggcccgac gatgtgctcg tcgcggcgat cgacgcggcg
     8101 cacgccgccg gagccagggt cacggcacac gtcttcggcg ccgacgccct ccccgggctc
     8161 atccgcgccg ggatcgactg catcgagcac ggccccgggc tcaccgacga cctcatcacg
     8221 gagatggccc gccgcggtac cgcgctggtg cccacgctga tcaacatcga gacgttcccc
     8281 ggcatcgccg acgcggccac caagtacccg acctacgcgg ctcacatgcg cgagctgcac
     8341 gccggcacgg caggcgttgt ccgcagggcc gtcgaggccg gggtccccgt gttcgccggc
     8401 accgatgcgg gcggcgggat cgtgcacggc cggatcgccg acgagatcat cgcgctgcac
     8461 agcgccgggc tgtccgcagc cgacgcgctc ggcgccgcga gctggtcggc ccgggactgg
     8521 ctcggccggc ccgggctgca tctgggcgcc cgcgccgacc tggtggtcta ccgatccgac
     8581 ccgcgcgccg acctctccgt cctgcgggag cccgcgctcg tcatgctgcg cggcaggccg
     8641 gtgcacgcca ccggctgacc cgccagcgcc cgtcggcccg tccgtgaatc cgcgcgggaa
     8701 gatcacccca ggtgacggct ccggcgcacc gcgtcacgtg ccgataggac cacgatcatg
     8761 cacgagcctg tgcacgacgt ccgatggcca cggccggtag cgtcgggagc gtgggagcag
     8821 cggaggggcc cgacgaggag cgttcgcgag agcggagcga cagcgcggca agggacagcg
     8881 cggcgaggga cagtgacctg cccgacagca gcccgcctgg cggcagccgg cccgacacca
     8941 gcctgcccca cagcagcccg cccgacagca ccccgcctga caccagcggg
//
LOCUS       DASRSC010000159        11404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1853763,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000159 DASRSC010000000
VERSION     DASRSC010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11404
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1853763"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1308
                     /locus_tag="VFE39_07665"
     CDS             151..1308
                     /locus_tag="VFE39_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6258513.1"
                     /translation="MEPSDLADVLSAVRAFIRKEVVPLEEQIDAEDAIPERIIATCKE
                     MGLYGFAIPEEYGGLGMTLTEEAQLAFELGWTTPALRSLFGTNNGIAGQVLLVGGTEE
                     QKKSWLPRLASGEITASFGLTEADAGSDPSGLATTARRDGGDWVINGSKRYITNAPVA
                     DVLMVFARTNPEAAGNRGISTFLVPAGTPGLSIGPKDHKMGQFGAWTADVHLDDVRVP
                     AEALVGGDAGIDQGFLTAAKCLAHGRAHIAALCVGMAGRLVHESVEFAKTREQGGRPI
                     ASFQLIQGLIADSVTDHYAGRALVLDVARAYDAGTDKVQGPSAAKYFASEMVGRVADR
                     AVQVHGGAGYMRGVAVERFYRDARLFRIYEGTSQIQQVIIARQALGRAARG"
     gene            complement(1305..2693)
                     /locus_tag="VFE39_07670"
     CDS             complement(1305..2693)
                     /locus_tag="VFE39_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6258514.1"
                     /translation="MSTDRPTSEAVEGGDLSEFGYQQELRRSLTLRDLLVYGLVFMVP
                     IAPFAIFGVVFNASMGMVPLTYVIGFVAMIFTALSYREMSRAFPISGSVYAYARRGLH
                     PAAGFLAGWAILLDYLLVPTLLYVTGAAALGSVLPAVPQAVWVILFVVVNTVVNLLGI
                     ETTARTNKIFLLGELVVLALFVVLAVVAISRGVNGASWTFTPFFNPEVFQPSLIFAAL
                     SVAVLSFLGFDAISTMAEETRGGARVVGRATVIALTMVAALFIVQTYLAALLVPGQTE
                     FAGETATNEAFYTVAGLVGGEWFKLLVAIATALGWAIANALVAQAATSRLLFSMARDR
                     QLPTFLAHVHPTRRVPERAVLLVSVVSLVLGLFFVGQIGLLSSLVNFGALFSFLLLHL
                     SVAGWYLIKQRQRTYGLHLVVPLLGFVIIGYVLVNADANAKIGGLVWLAVGVVVLVVL
                     RVTGRATQLKVS"
     gene            complement(2690..3640)
                     /locus_tag="VFE39_07675"
     CDS             complement(2690..3640)
                     /locus_tag="VFE39_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009082225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HET6258515.1"
                     /translation="MTEAIPLTTPRALADAGRQGTVGFGEYETWYRITGELRRDRAPL
                     VTLHGGPGCTHDYLLAIADLAEQTGRAVIHYDQLGNGRSTHLPEKGTDFWTVELFLAE
                     LDNLLAALGIQGDYHLLGQSWGGMLAAEHAVRRPTGLRSLTIADSPASMELWVAEANR
                     LRDLLPPEVQAVLLAHEADETYDHPDYQKAMQVFYDRHVCRVLPYPPEVAATFAALDA
                     DPTVYRTMNGPTEFHVVGTLRSWSIIDRLDRIAVPTQLVSGAYDEATPACVQPFADAI
                     PDVRRHVFPESSHLPHVEEREAFMTLLTGFLATVDERVAG"
     gene            3792..4043
                     /locus_tag="VFE39_07680"
     CDS             3792..4043
                     /locus_tag="VFE39_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HET6258516.1"
                     /translation="MPSWTGWSPRSRSARLSRELLARVNVGPAIEPVTEELRRRAVPQ
                     QRALVDAIADGDPDAAGAVAREHFTITRDALRRAVDRVG"
     gene            complement(4160..4558)
                     /locus_tag="VFE39_07685"
     CDS             complement(4160..4558)
                     /locus_tag="VFE39_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 4"
                     /protein_id="HET6258517.1"
                     /translation="MKVESRIFEIITVFFFIAAIVYTVLTHEVVGVVGLFLTGGLSLI
                     IGTYFRFVSRRLEARPEDNPDAEVSDGAGDVGFFSPGSYWPIMVAGSAAIVGIGLAFF
                     YVWLLVIGGVLLLIAVGGLVFEYHIRPAEH"
     gene            complement(4569..5498)
                     /locus_tag="VFE39_07690"
     CDS             complement(4569..5498)
                     /locus_tag="VFE39_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HET6258518.1"
                     /translation="MGRGRVAKLAALAALIIPLTTGCSVDEVIRFGWPVGVTPQAEAM
                     RQLWTWSAIAALVVGAITWGAMFWAIAFHRKRKGDDDSPPRQTQYNLPIEIVFTVIPT
                     IIVAVLFGFTVNVQNYVDAHLPNPDVRVNITAFQWNWRFDYPDARAANGQPITTQGTS
                     ETIPLLVLPTDRSIQFTQRSTDVIHSFFVPEFLFKRDVFPYPQQNDQDNVWRIDRIDR
                     EGAFVGRCAELCGAYHAMMNFEVRAVSPALYDRWVTLREQVNPATGALYTAGEALGAL
                     NCGQLCTPEAYTTYPFNTDRTKRSASEPVTAGS"
     gene            5711..7636
                     /gene="asnB"
                     /locus_tag="VFE39_07695"
     CDS             5711..7636
                     /gene="asnB"
                     /locus_tag="VFE39_07695"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019815148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6258519.1"
                     /translation="MCGLLGLLTAGADAAARTEPVAAAMRCARHRGPDESGTWNDADV
                     AFGFNRLSFIDIEHSHQPMSYAEGRYTIVFNGEIYNYLELRDELTQQYGARFATEGDT
                     EAILAAYHYWGPAAVGRLRGMFAFLIWDAQERVLFGARDPFGIKPLFVATGPAGVAFA
                     SEKKSLLEMAPALGLGGAGGGVTADLDPAALHHYLLLQYVPEPATLHRAIRRVESGTH
                     LTVRPGGEVEYERYFTPTFHSGAAAGPAALHSRIAEALADSVAKHMRADVTVGAFLSG
                     GIDSTAIAALAKRHNPDLITFTTGFEREGYSEVDVAAESAAAIGVRHVIRTVKADEMM
                     AALPLIIWYLDDPVADPALVPLWFIAREARQHVKVVLSGEGADELFGGYTIYHEPLSL
                     APFEKVPGAVRGLLGRASTRLPEGMRGKDLLRRGALPLEERYYGNARIFRDEHLAGIL
                     RQYDPRRSHTEITAAHYRDSAAWDPVSRMQHVDLFTWLRGDILVKADKMTMANSLELR
                     VPFLDPEVFAVASGLPLSQKLAEGTTKYALRRALEGIVPPHVLHRRKLGFPVPIRHWL
                     RDEMYQWARDIIRDSQADHLVDLSAVQRMLDAHRAGPVDHSRRIWTLLVFLLWHGIFV
                     EQRIRPEIPEPAYPVRP"
     gene            complement(7703..8623)
                     /locus_tag="VFE39_07700"
     CDS             complement(7703..8623)
                     /locus_tag="VFE39_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HET6258520.1"
                     /translation="MHFPGRFVDQLVADQLDRLSLSFLVDDMVIRPGGVAGNIAYALG
                     VLGQAPLLVGAVGADFGEYRSRLEANGVDCSAVLVCADVNTARFVCTTDDDMRQLASF
                     YTGAMARSREISLAPFTGRVDLALIGANDPEAMLRHTDECRELGLPFAADPSQQLARM
                     EGPEIRRLVEGARYLLTNDYEWELLLKKTGWTEAQIAEKIEIRITTHGEKGVEIYGPE
                     TLKIGIVPETGKVDPTGVGDAFRAGFLAAVAGGLSMERSAQLGSLIAVQVLETDGPQE
                     WTWDRAHGLERLRDAYGPDAATEIERVLPA"
     gene            complement(8765..9127)
                     /locus_tag="VFE39_07705"
     CDS             complement(8765..9127)
                     /locus_tag="VFE39_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HET6258521.1"
                     /translation="MTVDNTTEVVTETHGVELTDAAALKARALLEQEGRDDMHLRIAV
                     QPGGCAGLRYQLFFDDRSLDGDLFREYNGLKVGVDRMSAPYLQGAVIDFVDSIEKQGF
                     TIDNPNAGGSCACGDSFN"
     gene            complement(9282..10400)
                     /locus_tag="VFE39_07710"
     CDS             complement(9282..10400)
                     /locus_tag="VFE39_07710"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET6258522.1"
                     /translation="MRVVVAPDSFGGTLSATAAADAIATGWRRSGPDDELRLLPLADG
                     GTGFVEVLHTALGGEWHELEVTGPFGDPVSARWLRIGSTAYLESAAACGLHLVPRERR
                     VPDTARTVTTRGVGELVAEARDAGVTEVVIGLGGSATTDGGAGMLAALGAVAVDASGA
                     PVPAGGAALAGCARLEGVPELGGVRLVAAADVDNPLLGEHGAAAVFGPQKGADDTAVA
                     ELDAALAHFADVLAGGLGADVRDDPGAGAAGGLGAALLACGARRVSGAGLVRELVGLD
                     AELDKADLAITGEGSFDWQSLRGKLITAVARGAADRGVPCVVLAGQVSVGRREAAAVG
                     VDSAYSVAEDAGGVEASMADPAGTLAALAERVAARWSR"
     gene            10434..11066
                     /locus_tag="VFE39_07715"
     CDS             10434..11066
                     /locus_tag="VFE39_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3043 domain-containing protein"
                     /protein_id="HET6258523.1"
                     /translation="MRFLRRSAPSTVGADPTDTDPAQGNGDSTRTAGKGRPTPKRREA
                     ETKRRGPAPAPPRTQREASRLARANRPDKEQRRRDAAERRERMAAGDDRVLMPRDRGP
                     VKAYIRDVVDSRPHLMGLFMPLAVIVVLSLLVQVPSVQQYLSMFSLVALSVMIIEGVY
                     LGATVTKKARAKFPDAGINPLTTGWYAFTRASQLRRLRIPKPRVARGANP"
     gene            complement(11099..11296)
                     /locus_tag="VFE39_07720"
     CDS             complement(11099..11296)
                     /locus_tag="VFE39_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258524.1"
                     /translation="MAYRWRYLNAAGEQVEGPDEQFDNQQLAEEWFAADWELLREIGV
                     DAVTLLDGNDEVYGPMSLHEA"
BASE COUNT         1767 a         4064 c         3969 g         1604 t
ORIGIN      
        1 gcgcaggagg agcgcgtcag cgccacggtg atccagaccg tcggcgcgaa ggggtacgac
       61 ggtttcctgc tcgcactggt ccactagccg gctccccgtt cccgggcggc cggcgcgccc
      121 ctcaggcaac gaacgacggg agagatcgac gtggagcctt ccgacctggc cgacgtgctg
      181 tccgcagtgc gcgcgttcat ccgcaaggag gtcgtgccgc tggaggagca gatcgacgcc
      241 gaggacgcca tcccggagcg gatcatcgcc acctgcaagg agatggggct ctacgggttc
      301 gcgatccccg aggagtacgg cgggctcggg atgacgctca ccgaggaggc acagctcgcg
      361 ttcgagctgg gctggaccac cccggcgctg cggtcgctgt tcggcaccaa caacggcatc
      421 gccggacagg tgctgctggt cggtggcacc gaggagcaga agaagtcgtg gctgccgcgg
      481 ctggcgtccg gtgagatcac ggcctcgttc gggctcaccg aggcggacgc gggctcggac
      541 ccatccgggc tcgcgaccac ggcgaggcgc gacggcggcg actgggtgat caacggttcc
      601 aagcgctaca tcaccaacgc acccgtcgcc gacgtgctca tggtgttcgc ccgcaccaat
      661 cccgaggccg ccggcaaccg cggcatctcg acgttcctgg tgcccgccgg cacgcccggg
      721 ctctccatcg ggccgaagga ccacaagatg ggccagttcg gcgcgtggac ggccgacgtc
      781 cacctcgacg acgtgcgcgt cccggccgag gcgctcgtcg gcggcgacgc cgggatcgac
      841 cagggcttcc tgaccgcggc gaagtgcctg gcccacgggc gggcacacat cgcggcactg
      901 tgcgtcggga tggcggggcg gttggtgcac gagtccgtgg agttcgcgaa gacccgcgag
      961 cagggcggtc ggcccatcgc gtcgttccag ctgatccaag gcctgatcgc cgactcggtc
     1021 accgatcact acgcgggacg cgcactcgtg ctcgacgtcg cgcgggccta cgacgccggc
     1081 accgacaagg tgcagggccc gtcggccgcc aagtacttcg ccagcgagat ggtgggccgg
     1141 gtggccgacc gcgccgtgca ggtgcacggc ggggccggct acatgcgggg ggtcgcggtg
     1201 gagcgtttct accgcgacgc ccggctgttc cggatctacg agggcaccag ccagatccag
     1261 caggtgatca tcgctcgcca ggcactgggg agagcggccc gcggctagct caccttcagc
     1321 tgtgtggcac gccctgtcac gcgcagcacg accagcacca ccacaccgac ggcgagccac
     1381 accagcccgc cgatcttggc gttggcgtcg gcgttcacca gcacgtaccc gatgatcacg
     1441 aagccgagca gcggcacgac gaggtgcagc ccgtacgtcc gctgccgctg cttgatcagg
     1501 taccagccgg ccaccgacag gtgcagcagc aggaacgaga acagcgcacc gaagttcacc
     1561 agcgacgaca gcagcccgat ctgcccgacg aagaacagcc cgagcaccag gctcaccacc
     1621 gacacgagca gcaccgcccg ctccggcacc cggcgggtgg ggtgcacgtg cgcgaggaac
     1681 gtcggcagct gccggtcgcg tgccatggag aacagcagcc gcgacgtggc agcctgcgcc
     1741 accagcgcgt tcgcgatggc ccacccgagt gcggtggcaa tggccaccaa taacttgaac
     1801 cactcaccac cgaccaggcc tgcaaccgtg tagaacgcct cgttggtggc cgtctcgccg
     1861 gcgaactccg tctgcccggg cacgagcaac gcggcgaggt aggtctgcac gatgaacagc
     1921 gcggccacca tcgtcagggc gatcacggtc gcccgcccga ccacccgggc gccgcccctg
     1981 gtctcctctg ccatcgtgct gatcgcgtcg aagccgagga acgacagcac ggcaaccgac
     2041 agggccgcga agatcaggct gggctggaac acctccgggt tgaagaacgg cgtgaacgtc
     2101 cagctcgccc cgttgacccc gcgcgagatg gccaccaccg ccagcacgac gaacagcgcc
     2161 agcaccacca gctcgcccag caggaagatc ttgttcgtgc gcgcggtggt ctcgatcccg
     2221 agcaggttca ccacggtgtt gaccaccacg aagaggatca cccacaccgc ttgcggcacc
     2281 gcgggcagca ccgaacccag cgcagcggca ccggtgacgt agagcagggt ggggacgagc
     2341 aggtagtcga gcaggatcgc ccagccggcg aggaacccgg ccgccgggtg gaggccgcgg
     2401 cgtgcgtagg cgtacacgga gcccgagatc gggaacgcgc gcgacatctc ccggtagctc
     2461 aaggccgtga agatcatggc gacgaagccg atgacgtagg tgagcggcac cattcccatc
     2521 gacgcgttga agaccacgcc gaagatcgcg aacggcgcga tcggcaccat gaacacgagc
     2581 ccgtatacca gcaggtcgcg cagggtgagg ctgcggcgca gctcctgctg gtagccgaac
     2641 tcgctcaggt caccgccctc gacagcctcg ctggtgggtc ggtcagtact catccggcca
     2701 cccgctcgtc caccgtggcc aggaagcctg tgagcagcgt catgaaggcc tctcgttcct
     2761 cgacgtgggg caggtgactg gactcgggga agacgtgccg gcggacgtcg gggatggcgt
     2821 ccgcgaacgg ctgcacgcat gcgggtgtcg cttcgtcata cgcaccggag acgagctgcg
     2881 tggggacggc gatccggtcg agccgatcga tgatcgacca cgaccggagc gtcccgacga
     2941 cgtggaactc agtgggtccg ttcatggtgc ggtagaccgt ggggtcggca tcgagggcgg
     3001 cgaacgtggc cgccacctcc ggcgggtagg gcagcacccg gcacacgtgc cggtcgtaga
     3061 agacctgcat cgccttctgg tagtccgggt ggtcgtaggt ctcgtccgcc tcgtgggcga
     3121 gcagcacggc ctgcacctcg ggcggcagca ggtcgcgcag ccggttggcc tcggccaccc
     3181 acagctccat cgacgcgggc gagtcggcga tcgtcaggct gcgcagtccg gtcgggcgcc
     3241 gcaccgcgtg ctcggcagcg agcatgccgc cccaggactg ccccaacagg tggtagtcgc
     3301 cctggatccc cagcgcggcc agcaggttgt ccagctcggc gaggaacagc tcgacggtcc
     3361 agaagtcggt gcccttctcg ggcagatggg tggaccggcc gttgcccagc tggtcgtagt
     3421 ggatcaccgc gcgaccggtc tgctcggcca ggtcggcgat cgccagtagg tagtcgtggg
     3481 tgcagccggg gccgccgtgc agggtgacga gcggtgcccg gtcccggcgg agctcgcccg
     3541 tgatccggta ccaggtctcg tactcgccga acccgacggt gccctgccga ccggcgtccg
     3601 cgagtgcgcg aggtgtggtc agggggatcg cctccgtcag tcggcgagat catcactcgt
     3661 attcggtact cgaacaagta ccgttgagag cccaggatgg gacaattcgg atatgtcggc
     3721 cgagaaggcc cgcgacgaca cctggctcgg ttcggtaccg atgggccgca gcgtcgtcgg
     3781 gcgcagtgtc gatgccgtca tggaccggct ggtcaccgcg atcgcggtcg gcgaggctct
     3841 cccgcgagct gctggcccgt gtcaacgtcg ggccggccat cgagccggtc accgaggagc
     3901 tccggcgacg tgcggtgccc cagcagcggg cgctggtgga cgccattgcg gacggcgatc
     3961 ccgacgcggc gggggccgtc gcgcgcgagc acttcacgat cacccgtgac gcgctgcggc
     4021 gggccgtcga ccgggtcggc tgagcgcccg tccgagggcg actgcggcgc gacgagcggg
     4081 gtccgtcctt ctgatccgag agctgtgaca agccgtggaa ccgggccggc ccccgccggt
     4141 ccggccccgg gcggctgcgt cagtgctcgg cgggccggat gtggtactcg aacaccagac
     4201 cgccgacggc gatcagcagc aggacgccgc cgatcaccag gagccacacg tagaagaagg
     4261 ccagcccgat gccgacgatc gccgccgacc cggccaccat gatcggccag tagctgccgg
     4321 gggagaagaa gccgacatcg cccgcgccgt cggagacctc agcgtccggg ttgtcctccg
     4381 gacgggcctc caggcgtcgc gagacgaacc ggaagtacgt gccgatgatc agcgacagtc
     4441 cgccggtgag gaacagcccg accacgccga cgacctcatg ggtgagcacc gtgtagacaa
     4501 tggccgcgat gaagaagaag accgtgatga tctcgaagat gcgggattcg accttcatgg
     4561 cgtgcctctc agctccccgc ggtgaccggc tcggacgccg agcgcttcgt gcggtcggtg
     4621 ttgaacgggt aggtggtgta ggcctccggc gtgcacagct ggccgcagtt gagcgcaccc
     4681 agcgcctcgc ccgcggtgta gagcgcaccc gtggccgggt taacctgctc gcgcagcgtg
     4741 acccaccggt cgtacagcgc cgggctcacg gccctgacct cgaagttcat catggcgtgg
     4801 taagcgccgc acagctcggc gcagcgaccg acgaacgcgc cctcacggtc gatgcggtcg
     4861 atccgccaga cgttgtcctg gtcgttctgc tgggggtacg ggaacacgtc gcgcttgaac
     4921 aggaactccg gcacgaagaa gctgtggatc acgtcggtgg agcgctgcgt gaactggatg
     4981 cttcgatccg tgggcagcac aagcagcggg atcgtctcgg acgtgccctg tgtggtgatc
     5041 ggctgcccgt tcgccgcccg ggcgtcgggg tagtcgaacc gccagttcca ctggaacgct
     5101 gtaatgttga cccggacgtc gggattgggc agatgcgcgt cgacgtagtt ctggacgttg
     5161 acggtgaagc cgaagagcac cgcgacgatg atcgtcggga tcacggtgaa gacgatctcg
     5221 atcggcaggt tgtactgcgt ctggcgcggc gggctgtcgt cgtcgccctt gcgcttgcgg
     5281 tggaacgcga tcgcccagaa catcgcgccc caggtgatcg cacccaccac gagtgcggcg
     5341 atcgccgacc aggtccacag ctggcgcatc gcctcggcct gcggcgtgac gccgaccggc
     5401 cagccgaagc ggatgacctc gtccaccgag caaccggtgg tcagtgggat gatcagagcg
     5461 gccagggcgg ccagcttcgc cacccgtccg cggcccacgc gaacggaatc gcggtgatct
     5521 cccccgcgct gtgctcggcc cactgcgcgc cgcctcccca acccgtcgtg ttccgtcggc
     5581 cgccggacgc cccggctgcg cgagcctgca cgcgccttcg acctcgcgta gagactaacc
     5641 gagcagaccc tcgatccggg tgtcgggtcg gcaccgggcc ggcggtgagt cgtctgcata
     5701 ctggctcgct gtgtgtggac tgctgggact gctgaccgcc ggggccgacg ctgccgcccg
     5761 gaccgaaccg gttgctgcag ccatgcgctg cgcgcgccac cgggggccgg acgagagcgg
     5821 cacctggaac gacgcggacg tcgcgttcgg gttcaaccgg ctctcgttca tcgacatcga
     5881 gcactcgcac cagccgatga gctatgcgga gggccgctac acgatcgtct tcaacggcga
     5941 gatctacaac tacctcgagc tccgcgacga gctcacccag cagtacgggg ccaggttcgc
     6001 caccgagggc gacaccgagg cgattctggc ggcctaccac tactggggcc cggccgcggt
     6061 cggcaggctg cgcgggatgt tcgcgttcct catctgggac gcccaggagc gcgtgctgtt
     6121 cggcgctcgc gacccgttcg ggatcaagcc gctgttcgtg gccaccgggc cggcgggagt
     6181 cgccttcgcg agcgagaaga agagcctgct ggagatggcg cccgcgctgg gtctgggagg
     6241 ggcgggcggg ggtgtcaccg ccgacctgga cccggcggcg ctgcaccact acctgctgct
     6301 gcagtacgtg ccggagcccg ccacgctgca ccgcgcgatc cggcgcgtcg agtccggcac
     6361 gcacctcacg gtccgtcccg ggggggaggt ggagtacgag cgctacttca caccgacgtt
     6421 ccactccggc gccgccgccg ggcccgccgc cctgcactcc cggatcgccg aagcgctcgc
     6481 cgacagcgtg gccaagcaca tgcgcgccga cgtcaccgtc ggtgcgttcc tgtccggcgg
     6541 gatcgactcc acggcgatcg ccgcgctggc caagcggcac aacccggacc tgatcacgtt
     6601 caccaccggt ttcgaacgcg agggctactc ggaggtggac gtcgcggccg agtcggccgc
     6661 cgcgatcggg gtgcggcacg tcatccgcac cgtcaaggcc gacgagatga tggcggcgct
     6721 cccgctcatc atctggtacc tggacgaccc ggtggccgac cccgcgctcg tcccgctctg
     6781 gttcatcgcg cgtgaggccc gccagcacgt caaggtggtg ctgtcgggag agggcgcgga
     6841 cgagctgttc ggcggataca cgatctacca cgagccgctg tcgctcgcgc ccttcgagaa
     6901 ggtgccaggg gccgtgcggg ggttgctcgg ccgcgcctcg acgcgcctgc ccgagggcat
     6961 gcggggcaag gacctgctgc gccgcggggc actcccgctg gaggagcgct actacggcaa
     7021 cgcccgcatc ttccgcgacg aacatctcgc cgggatcctg cgccagtacg atccccgccg
     7081 ctcgcacacc gagatcaccg ccgcgcacta ccgcgactcc gcggcgtggg acccggtgtc
     7141 gcggatgcag cacgtcgacc tgttcacgtg gctgcgcggg gacatcctcg tgaaggccga
     7201 caagatgacg atggccaact ccctcgagct gcgggtgccg ttcctcgacc ccgaggtgtt
     7261 cgccgtggcg tccgggctgc cgctgtcgca gaagctggcc gagggcacca ccaagtacgc
     7321 gctgcgcagg gcccttgagg gcatcgtccc cccgcacgta ctgcatcggc gcaagctcgg
     7381 cttccccgtg ccgatccggc actggctgcg cgacgagatg taccagtggg ctcgcgacat
     7441 catccgcgac tcccaggccg accacctcgt cgacctctcg gccgtgcagc gcatgctcga
     7501 cgcccaccgc gccggtccgg tcgaccacag ccgccggatc tggacgctgc tggtcttcct
     7561 gctgtggcac gggatcttcg tcgagcagcg catcaggccg gagatcccgg agccggccta
     7621 cccggtccgc ccctgacccc tgatggtggc gagatccgcc gatccgccct cggaagggcg
     7681 gatcggcgga tctcgtgtgt cgtcaggccg gcaggacccg ctcgatctcg gtggctgcgt
     7741 cagggccgta ggcatcgcgg agccgctcca ggccgtgcgc ccggtcccag gtccactcct
     7801 gcgggccgtc ggtctccagc acctggacgg cgatgaggga gccgagctgc gccgagcgct
     7861 ccatggagag gccgcccgcc accgcggcca ggaacccggc acggaacgcg tcgcccacgc
     7921 ccgtcgggtc gaccttgccg gtctcgggga cgatgcctat cttgagcgtc tccggcccgt
     7981 agatctccac gcccttctcc ccgtgcgtgg tgatccggat ctcgatcttc tccgcgatct
     8041 gagcctcggt ccagccggtc ttcttgagca ggagctccca ctcgtagtcg ttggtgagca
     8101 gataacgcgc gccctcgacg agcctgcgga tctccgggcc ctccatgcgc gccagttgct
     8161 gcgacgggtc ggccgcgaag ggcaggccca gctcgcggca ctcgtccgtg tggcgcagca
     8221 tcgcctccgg gtcgttggct ccgatgagtg ccaggtcgac ccgcccggtg aacggggcga
     8281 gggagatctc ccgggaccgg gccatggcgc cggtgtagaa cgacgcgagc tgccgcatgt
     8341 cgtcgtcggt ggtgcagacg aaccgagcgg tgttgacgtc cgcgcagacg aggacggccg
     8401 agcagtcgac gccgttcgcc tcgagccgcg accggtactc accgaagtcg gccccgaccg
     8461 caccgaccag cagcggcgcc tgacctagca caccgagcgc gtaggcgatg ttgccggcga
     8521 cgccgcccgg ccggatcacc atgtcgtcga cgaggaagct cagcgagagt cggtcgagct
     8581 gatcggcgac gagctggtcg acgaaccgac ctgggaagtg catgagttga tcggtcgcga
     8641 tggacccggt tacggcgata ggcacggcca ggcaccctac cggtccggag atacagcaaa
     8701 ccccggctgg tgagccaaag ctcgcagccg gggttcgtgc cggatcacaa ctgccggacg
     8761 accgtcagtt gaacgagtcg ccacaggcgc aggaaccgcc cgcgttcggg ttgtcgatcg
     8821 tgaagccctg cttctcgatg ctgtcgacga agtcgatgac cgcaccctgc agatagggcg
     8881 cgctcatccg gtcgaccccg accttgaggc cgttgtactc gcggaacagg tcgccgtcga
     8941 gcgaccggtc atcgaagaac agctggtagc gcaggcctgc gcagccaccc ggctgaacgg
     9001 cgatgcgcag atgcatgtcg tcacggccct cctgctcgag cagggccctg gccttcaggg
     9061 cggcggcgtc ggtcagctcg acaccgtgcg tctcggtgac gacctcggtg gtgttatcga
     9121 cggtcatggc tctcccaaca acgagcgcgg cactgctgtt ccggtccgcg gcgtgtttcc
     9181 ggtccggggc gtgttccggt tctgcggtca tactatgccc ttgcaccccc cgcctgcctt
     9241 cccgtgggcg ccggggtgat ccgatccacc tcccgggtga cctaccggct ccagcgagca
     9301 gcgacgcgct ccgcgagcgc ggccagggtg cctgcggggt cggccatcga cgcctccacg
     9361 ccgccggcgt cctcggccac cgagtacgcc gagtcgacgc cgaccgccgc cgcctcccgc
     9421 ctgccgacgg acacctgacc ggccagcacg acacacggca ccccgcggtc cgccgccccc
     9481 ctcgccacgg cggtgatgag cttcccgcgc agcgactgcc agtcgaagct gccctcgccc
     9541 gtgatggcga gatcggcctt gtcgagctct gcgtccagcc cgacgagctc gcgcactagg
     9601 cctgccccgg acacccgacg ggcgccgcag gccaggagcg cggctccgag gccgcccgcc
     9661 gctccggctc ctggatcgtc gcggacgtcc gcgccgagcc cgccggccag cacgtcggcg
     9721 aaatgggcca gggccgcgtc cagctcggcg acggccgtgt cgtcggcgcc cttctgcggc
     9781 ccgaagacgg cggcggcgcc gtgctcgccg agcagcgggt tgtcgacgtc ggcggccgcg
     9841 acgagccgga cacctcccag ctcgggtacg ccctcgagcc gggcgcagcc ggcgagcgcc
     9901 gctcccccgg ccggtacggg cgcgccggac gcgtcgaccg ccaccgcgcc gagcgcggcg
     9961 agcatcccgg cccccccgtc ggtggtggcc gagccaccca gtccgatgac gacctccgtg
    10021 acgcccgcgt cccgggcctc ggcgacgagc tcgccgacgc cgcgggtggt gaccgtccgg
    10081 gcggtgtccg ggacgcggcg ctcccgcggc acgaggtgca ggccgcatgc cgccgccgac
    10141 tccaggtacg cggtcgaccc gatgcgcagc cagcgtgctg aaaccgggtc cccgaacggg
    10201 ccggtgacct ccaactcgtg ccactcgccg ccgagggccg tgtgcagcac ctcgacgaac
    10261 ccggtaccgc cgtcggccag cggcaacagc cgcagctcgt cgtcgggccc gctacgccgc
    10321 cagcccgtcg cgatcgcgtc ggccgcagcc gtcgcgctca aggtgccgcc aaatgagtcc
    10381 ggagccacga ccactcgcat gacgagcacc gtatcgcccg tatggttacg tgcgtgaggt
    10441 tcctgcgccg ctccgcgccg tccaccgtcg gtgccgaccc gaccgacacc gacccggcgc
    10501 agggcaacgg cgactccacc cgcaccgcgg gcaagggccg tcccacgccg aagcgccgcg
    10561 aggcggagac caagcgccgg ggtccggcgc cggccccgcc ccgcacccag cgcgaggcct
    10621 cccggctcgc cagggccaac cggccggaca aggagcagcg ccgccgcgat gccgccgagc
    10681 ggcgcgagcg gatggctgcc ggcgacgaca gggtcctcat gccccgcgac cgcgggccgg
    10741 tgaaggccta catccgcgac gtcgtggatt cccggccgca cctcatgggc ctgttcatgc
    10801 cgctcgccgt gatcgtggtg ctgtcgctgc tcgtgcaggt gccgtccgtg cagcagtacc
    10861 tcagcatgtt cagcctggtc gcgctctccg tgatgatcat cgaaggggtc tacctgggag
    10921 cgacggtcac gaagaaggcg cgcgcgaagt tcccggatgc cgggatcaac ccgctgacga
    10981 ccggctggta cgccttcacc cgcgccagcc agctccgacg gctacggatc ccgaagccgc
    11041 gggtggcgcg gggcgccaac ccctgacggc ggcacggtcc cagcggttga tccggtgctc
    11101 aggcctcgtg caggctcatc gggccgtaga cctcgtcatt gccgtccagc agcgtgacgg
    11161 catccacgcc gatctcgcgc agcaactccc agtcggccgc gaaccactcc tcggcgagct
    11221 gctggttgtc gaactgctcg tcgggaccct cgacctgctc gccagccgcg ttcaggtacc
    11281 gccaccggta cgccacggcc cggaggttag cgtgacgagg tggtcgtcgc ccgtcgcacg
    11341 ctcgtgctcg gcggcacgcg gtcaggcaag tcgcgctacg cggaggacct gctggccgcc
    11401 gatg
//
LOCUS       DASRSC010000160        11737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3001525,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000160 DASRSC010000000
VERSION     DASRSC010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11737
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3001525"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /gene="serC"
                     /locus_tag="VFE39_07725"
     CDS             complement(<1..1043)
                     /gene="serC"
                     /locus_tag="VFE39_07725"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HET6258525.1"
                     /translation="MTASTSTALQIPADLRPGDGHFGCGPSKVRPEQLAALADAGAAV
                     MGTSHRQKPVKSLVGRLRSGLAELFSLPEGYQVVLGNGGSTAFWDAAAFGLVRDRALH
                     LAYGEFSSKFADSTRGAPFLADPVVIKAEAGSAPVPAADPTCDVLAWAHNETSTGVAV
                     PVVRPEGAIDGQLVTIDATSAAGGLPVDVRQADAYYFAPQKGFASDGGLWIALLSPAA
                     LDRVAELAASDRWIPPFLSLATAVDNSLKDQTYNTPAIATLFLMAEQIDWMLGLGGLE
                     ACVARTADSSGRLYSWAEKSSFATPFVADPAHRSQVVGTIDFADSVDAAAVAKTLRAN
                     GIVDTEPYRKLGR"
     gene            1346..3442
                     /locus_tag="VFE39_07730"
     CDS             1346..3442
                     /locus_tag="VFE39_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258526.1"
                     /translation="MVASAGVATAPPAFRSVLGVRPRLVLASSLMLFVELALIRWTGS
                     NVVHLSYFSNFVLLGSFLGIGLGFLRAERARYRPFYSPIALAVLVLFVLAFPVTVDRG
                     QDDTVIYFTSLTTTGPPTWLVLPIVFLAAAVITAGPAELVGRCFTELPRLTAYRFDLI
                     GSLIGIAAFTGLSFLGAPPVWWGVIVAVAFAVLLVPAPDRTAVLVAVVLVGVPSALVV
                     AALWTESETPGARWSPYYKIQTGSRDVNGVPIVDIAANGVPHQQAIPAEARVAFEPQY
                     GLPYERSSPIQGGDLGNVLVIGAGSGTDVAIALRNGAQHVDAVEIDPGLLQIGREQHP
                     DRPYDDPRVDLHIDDGRAFLSRTDARYDLILFALPDSLTLVQGASSLRLESYLFTEEA
                     FASAREHLAPGGVLSMYNYYREGWLIDRLALTAQTVFGHAPCVDVVGGELGQAVVTVG
                     LTSADQSCAAAWGGPSADTPAPATDQRPFLYLFGATIPALYLVTIGLILLVSVVAVAV
                     VGGAGNIGGSYRRMRPYTDLFLLGAGFLLLQTKSIAGFALLFGTTWVVNAIVFAGVLL
                     AVLAAVEVTRRFRTPPLPVMYGVLFAGLLLSWLVPEAWLLAMPVPLRAVTAVVLAFLP
                     IFAANVVFAKRFTETADGTAAFGANLLGAMVGGCLEYLALLVGFPGLLLVAALLYAGA
                     FALAPRAALVPSPGLR"
     gene            3546..4826
                     /locus_tag="VFE39_07735"
     CDS             3546..4826
                     /locus_tag="VFE39_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6258527.1"
                     /translation="MTEASTPIRTQRVYRIHPAWWAAAVTFLALVGAAGFRAVPGVLM
                     DPLHDEFGWSISTISLAAAVNMALYGLTAPFAAALMERFGIRYVVTAALVVVAAGSGL
                     TVFMTASWQLVLCWGVLVGLGTGSMALALVATVTGRWFVARRGLVSGVLTAGGAAGGL
                     VFLPLVSQLAAAHGWRPAALAVAAAALGVAPLVLWLLRDRPRDIGAAPYGGTEADDVE
                     PVRSGAARLAMRALATAARTRPFWYLAAGMMICGATTMGLIQPHFIPAAHDHGMPQTT
                     AAGLLALVGIFDVAGTIASGWLTDRIDPRLLLLAYYVLRGLSLLALPTLFGPDIKANM
                     LAFIVFYGLDWVATIPPTMALCRELFGAAAPVVFGWVFASHQVGAALMALGAGIVRDE
                     LGAYDTAWYVGGALCVVAGVLSLLVGRAPAPAPV"
     gene            complement(4848..5945)
                     /locus_tag="VFE39_07740"
                     /pseudo
     CDS             complement(4848..5945)
                     /locus_tag="VFE39_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase 2"
     gene            6323..6973
                     /gene="pdxH"
                     /locus_tag="VFE39_07745"
     CDS             6323..6973
                     /gene="pdxH"
                     /locus_tag="VFE39_07745"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HET6258528.1"
                     /translation="MAERLATMRVDYDCEGFDIDALAPTWHEQLARWLVEAEAAGVAE
                     ANAMVLATTGEDSRPSSRTVLCKGVDARGVVFFTNYTSAKSHDLRVSRYASVTFPWYA
                     LHRQVHIRGPVELVTPAETQEYWASRPRGSQLGAWASAQSTVVRDRRALDDALEVVSQ
                     RFAAETVVPAPPHWGGWRLTPEQVEFWQGRENRMHDRLRFEVDGHERTWTVRRLAP"
     gene            7045..8448
                     /locus_tag="VFE39_07750"
     CDS             7045..8448
                     /locus_tag="VFE39_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6258529.1"
                     /translation="MTPGPEEPTRPNRLVQEGPTRQINTGPDAPTRPTGAAPTSATRR
                     ITGSVRGVLADTRPLRTPAFRRLWLAGVITVVGAQLSVVAVPTQIYQLTGSSAYVGLT
                     GLFGLVPLVVFGLWGGAIADVVDRRTVLLASGGAIALSSLALWVTAASGAGGVWAVLV
                     LFSVQTAFLAVNQPTRSAVIPRLLPVDQLPAANALNMTVVQIGAIAGPLLAGVLIPVV
                     GLPTLYLLDAVALLATLWATWRLPAVPPGAAMTRRAGLREVIDGFRYVAMHKVLLVSF
                     LIDVVAMGFGMPRVVFPETAHTVFGDPVSGGGALGLLFAAIPIGMVVAGLFSGRLTRV
                     RRQGVAVTFAICTWGLGVGLFGLTGSLWFAVLFLAVAGAGDMVSSTFRNSMLQTVATD
                     EMRGRMQGVFIVVVVGGPRLADLWHGPAADAIGPSAAAAVGGFAVIVFAIVVVAVFPM
                     FWRYRAPAAAAAPPKVV"
     gene            8523..9881
                     /locus_tag="VFE39_07755"
     CDS             8523..9881
                     /locus_tag="VFE39_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6258530.1"
                     /translation="MTDVSAVTAQPELKRAIGPKLLLFFVIGDILGTGIYALTGSVAG
                     VIGGALWVPFVLAFIVAFLTAFSYLELVGKYPRAAGAALYTHKAFGINFLTFMVTFTV
                     MASGVTSAATAAKAFGDTYLRQFIELPTWAVAAVFIAGLALINFRGVGESVKANVVLT
                     IIELSGLLLIIAVGIYAVAGGAGEPSRLVEINTGETTAFIAITAAASLAFFAMVGFED
                     SVNMAEECHNPPRIFPRAMLLGMGVAATIYVIIAVLSSMLVPAADLAAAKSGALFRVL
                     EVGAPGFPLGLFAVIGLFAVVNSALINMLMASRLLYGMSNERILPRVFGMVHEGRRTP
                     WVAIAFTSAIAIILVASVDITELGGTTALLLLAVFTIVNIAVLVLRRDKVDHDHFRAP
                     TWSPYVGIVLCGFLATPLSGRPLQQFVLAGILLAVGAVLWVINYLVVGRVDVDPEQLA
                     KG"
     gene            9978..11276
                     /locus_tag="VFE39_07760"
     CDS             9978..11276
                     /locus_tag="VFE39_07760"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HET6258531.1"
                     /translation="MADDTKNAVLHHPNGEHEMEIQAATEGASAFDISKLLPATGLVT
                     LDSGYGNTAACSSAITYIDGDAGILRYRGYPIDQLAESSTFLETSYLLIYGELPSKSE
                     LEAFTNKISRHTLLHEDLKRFFDGFPRDAHPMPVLSSAVSALSTFYQDSLDPFDQDAV
                     ELSTVRLLAKVPTIAAYAYKKSIGQPFLYPDNSLGLVENFLRMTFGFPAEPYEIDPDF
                     ARALDMLLILHADHEQNCSTSTVRLVGSSQANLFASISAGINALFGPLHGGANQAVLE
                     MLQRIRDVEDGNVDAFVERVKKREKGARLMGFGHRVYKNYDPRAKIVKQSADEILKKL
                     GVTDQLLDIAKALEERALADDYFVERKLYPNVDFYTGVIYRAMGFPTKMFTVLFALGR
                     LPGWIAHWREMINDPTTKIGRPRQLYIGSTERSYVRMGDR"
     gene            11394..>11737
                     /locus_tag="VFE39_07765"
     CDS             11394..>11737
                     /locus_tag="VFE39_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HET6258532.1"
                     /translation="MDDTAVVWFRRDLRVLDQPTFLAAADAASRSLALFVLDPALLVP
                     SGRARRTFLYRSLRALDDTLDGRLLVVRGDPADVVPRVSAGIDAATVHVAADFGPYGT
                     RRDEAVEKVLADA"
BASE COUNT         1525 a         4087 c         4137 g         1988 t
ORIGIN      
        1 ttgcggccga gcttgcggta gggctcggtg tccacgatgc cgttggcgcg cagcgtcttg
       61 gccacggccg cggcgtccac cgagtcggcg aagtcgatcg tgccgaccac ctgggagcgg
      121 tgcgcgggat cggcgacgaa cggcgtggcg aacgaggact tctcggccca ggagtagagg
      181 cggcccgagg agtcggcggt gcgcgcgacg cacgcctcga ggccgccgag cccgagcatc
      241 cagtcgatct gctcggccat caggaacagg gtggcgatag ccggcgtgtt gtaggtctgg
      301 tccttgagcg agttgtcgac ggcggtcgcg agcgacagga acggcgggat ccaccggtcg
      361 gacgcggcca gctcggccac ccggtccagg gcggccgggc tcagcagtgc gatccacagc
      421 ccgccgtcag aggcgaagcc cttctggggt gcgaagtagt acgcgtccgc ctggcggacg
      481 tccaccggca ggccgcctgc cgcggaggtg gcgtcgatcg tgacgagttg gccgtcgatc
      541 gcgccctccg ggcgcacgac cggcacggcg acgcccgtcg aggtctcgtt gtgggcccag
      601 gccagcacgt cgcacgtcgg gtccgcggcg gggacggggg cgctgcccgc ctcggccttg
      661 atcaccacgg ggtccgcgag gaagggggca ccgcgggtgg agtcggcgaa cttggacgag
      721 aactcgccgt aggccaggtg gagcgcccga tcgcggacca ggccgaaggc ggccgcgtcc
      781 cagaacgccg tggacccgcc gttgccgagc acgacctggt agccctcggg cagtgagaac
      841 agctcggcca gcccggagcg caatcggccg accagcgact tcaccggttt ctggcggtgc
      901 gacgtaccca tgacggcggc gccggcgtcg gcgagcgcgg cgagctgttc cgggcggacc
      961 ttggaggggc cgcagccgaa gtgcccgtcg ccgggcctga ggtcggcggg gatctgcagg
     1021 gcggtagatg tcgaagcggt cacggcccca gtctcctcca atccgggccg gctgctggag
     1081 acgccccagg tgccgcacgg actgcggcgg cagcgtgacc accagtgctg ccgggtcatg
     1141 ccgctcctgt ctcttccagt cgccgtcggt cagcaccgga caggtcttgg ccttctcgcg
     1201 atcacgacgg tgggcgcgtc gaggttcccg cgtgtcacca cccgcgcggc ttcggcggcc
     1261 ggtctcaacg cgatccgcga gaggatcgcg tacttacagt cccgatgtcc ggcctcccga
     1321 ggcgtcaccc gtaccctccg gccgcatggt cgcctccgcc ggtgtcgcca ccgcgccccc
     1381 cgccttccgg tcggtgctcg gcgtccggcc ccggctggtg ctggcgagct cgttgatgct
     1441 gttcgtcgag ctggcgttga tccggtggac cggctcgaac gtcgttcacc tgagctactt
     1501 ctccaacttc gtgttgctcg gctcgttcct ggggatcggc ctgggcttcc tgcgcgcaga
     1561 acgggcgcgg taccgcccct tctactcgcc gatcgcgctg gcggtgctgg tcctgttcgt
     1621 cctggcgttt ccggtgaccg tggaccgggg ccaggacgac acggtcatct acttcaccag
     1681 cctcaccacg acggggccgc ccacctggct cgtgctgccc atcgtgttcc tggccgcagc
     1741 agtgatcacg gctggaccgg ccgagctcgt gggccggtgc ttcaccgagc tgccgcggct
     1801 caccgcgtac cgcttcgatc tgatcggcag cctgatcggg atcgcggcct tcaccggcct
     1861 gtccttcctc ggagcaccgc cggtctggtg gggcgtgatc gtcgcggtgg cgttcgctgt
     1921 gcttctggta cccgcgccgg accggaccgc ggtgctggtc gcggtggtgc tggtgggggt
     1981 gccgtcggcg ctggtcgtgg ccgcgctgtg gacggagtcg gagacgccgg gggcgaggtg
     2041 gtccccgtac tacaagatcc agaccggctc ccgcgacgtg aacggggtgc cgatcgtcga
     2101 catcgcggcg aacggggtgc ctcatcagca ggccattccc gccgaggcgc gggtcgcgtt
     2161 cgagccccag tacgggctgc cctacgagcg gtccagcccc atacagggcg gggacctcgg
     2221 gaacgtgctc gtcatcgggg cgggcagcgg caccgacgtc gcgatcgcgc tgcgcaacgg
     2281 cgcccagcac gtcgacgccg tggagatcga cccggggctg ctgcagatcg gccgcgagca
     2341 gcacccggac cggccctacg acgacccgcg cgtggacctg cacatcgacg acggccgggc
     2401 cttcctctcg cgcaccgatg cccgctacga cctgattctc ttcgcgctgc ccgactccct
     2461 caccctggtg cagggcgcga gctcgctgcg gctggagagc tacctcttca ccgaggaggc
     2521 cttcgcctcc gcgcgcgagc acctcgcacc cggcggggtt ttgtcgatgt acaactacta
     2581 ccgcgagggt tggttgatcg accggctggc gctcaccgcg cagaccgtgt tcggccacgc
     2641 cccgtgcgtc gacgtggtcg gcggggagct cggccaggcg gtggtcacgg tcggcctgac
     2701 gtccgcggac cagagctgcg ccgctgcctg gggcggcccg tccgccgaca ctccggcgcc
     2761 cgcgacggac cagcgcccgt tcctctacct gttcggcgcc accatcccgg ccctctatct
     2821 cgtgaccatc gggctgatcc tgctggtcag cgtggttgcg gtcgcggttg tcggaggagc
     2881 ggggaacatc ggcggctctt accggcggat gcgcccctac accgatctgt tcctgttggg
     2941 ggcgggtttc ctgctgctgc agaccaagag catcgcagga ttcgcactgc tgttcggcac
     3001 cacctgggtg gtgaacgcga tcgtcttcgc cggcgtgctg ctcgccgtgc tcgccgccgt
     3061 cgaggtgacg cggcggttcc gcacaccgcc gctgccggtc atgtacggcg tgctgttcgc
     3121 gggactgctg ctcagctggc tggtgccgga ggcgtggctg ctggccatgc cggtgccgct
     3181 gcgtgctgtc accgcggtgg tactggcctt cctgccgatc ttcgcggcca acgtggtgtt
     3241 cgcgaagcgg ttcaccgaga ccgccgacgg caccgccgcg ttcggggcca acctgctcgg
     3301 cgcgatggtg ggcgggtgtc tggagtacct ggcgctcctg gtcggcttcc ccgggctgct
     3361 gctcgtggcc gcgttgctct atgcaggcgc cttcgccctg gcgccccgcg ccgcgctggt
     3421 cccgtcacct ggattgcgat agatgcgccg ttaggtcggc gttgcgagtg atatggcggg
     3481 atctttccct atcgtgacca tcgggccact ttcctcccga tgaccgagcg gcaatgctgg
     3541 ctaccgtgac cgaagccagc acgccgatcc gcacgcagcg cgtgtatcgc atccatccgg
     3601 cctggtgggc cgccgccgtc accttcctgg cgctcgtcgg tgcggcgggg ttccgcgccg
     3661 tccccggtgt actgatggac ccgttgcacg acgagttcgg ctggtcgatc tccacgatct
     3721 cgctcgccgc ggctgtcaat atggccctct acggcctgac ggctccgttc gcggccgcgc
     3781 tgatggagcg cttcggcatc cggtacgtcg tcacggcggc gctggtcgtg gtggccgcgg
     3841 gcagcggcct caccgtgttc atgacggcga gctggcagct cgtcctgtgc tggggcgtgc
     3901 tcgtcgggct cggcacgggc tcgatggcgc tcgcgctcgt cgccacggtc accggacgct
     3961 ggttcgtcgc ccgccgcggc ctcgtctccg gtgtgttgac ggcgggcggg gccgcgggcg
     4021 ggctggtttt cctcccgctc gtgtcgcagc tcgccgccgc acacgggtgg cggccggccg
     4081 ccctcgccgt ggccgcagcg gcgctcggcg tcgccccgct ggtgctgtgg ctgctgcgcg
     4141 accgcccgcg tgacatcgga gcggcgccct acggcgggac agaggccgac gacgtcgagc
     4201 cggtgcggtc gggcgcggcc cggctcgcga tgcgcgccct ggccaccgcc gccaggacgc
     4261 gcccgttctg gtacctggcc gccgggatga tgatctgcgg tgccaccacg atgggcctga
     4321 tccagccgca cttcatcccc gctgcccacg accacggcat gccgcagacc acggccgcgg
     4381 ggctgctcgc gctcgtcggg atcttcgacg tcgccggcac gatcgcgtcg ggctggctca
     4441 ccgaccgtat cgacccccgg ctgctgctgc tcgcctacta cgtgctgcgc gggctctcgc
     4501 tgctcgcgct gccgacgctg ttcgggccgg acatcaaggc gaacatgctg gcgttcatcg
     4561 tgttctacgg gctggactgg gtggcgacga tcccgcccac catggcgctg tgccgggagc
     4621 tcttcggcgc cgccgcgccc gtcgtgttcg gctgggtgtt cgcgtcgcac caggtgggtg
     4681 cggcgctgat ggcactgggc gccgggatcg tgcgcgacga gctgggcgcg tacgacacgg
     4741 catggtacgt cggtggtgcg ctgtgcgtcg tggccggcgt gctctcgctg ctggtggggc
     4801 gcgcgccggc accggccccg gtgtgagcgg ggccggcccg agcccggtca gtcgtgcttg
     4861 atctcgtccc agccctcgac tgcggagggc ttgcgcgggc cgggtccgat gtactgggcg
     4921 gacggcctca cgagcttgct ctcgcgcttt tgttccatga tgtgcgccga ccagcccgcg
     4981 gtgcgggcgc tggtgaacat ggccggcatc atgtgcggcg gcacctcggc aaagtccagg
     5041 atcactgctg cccagaactc gacgttggtc tcgatggggt ggtcggggcg ccgctcgcgc
     5101 agctgggtga gcgccgcctg ctcgagcgcc accgccgcct cgtaacgcgg ggcattcagc
     5161 tcctgacagg tacggcgcag cacgcgggcg cgcgggtcct cggcccggta gacccggtgc
     5221 ccgaagccca tgagcttctc cttgcggtcg aggatgcccg tgaccagcgc cgctgggtcg
     5281 ccggaccgct cgaccgcctc gagcatcggc aggacgcggg cgggtgcgcc gccgtgcaga
     5341 gggccggaca tcgcaccgat cgcgcccgac agggacgccg cgacgtcggc gccggtggac
     5401 gcgatgaccc gggcggtgaa cgtggaggcg ttcatcccgt gctcggccgc cgacacccag
     5461 tacgcgtcca cggccttgat gtgcgccggg tccggctcgc cgcgccagcg cgtcatgaag
     5521 cgctcggtga tcgtggtgca ctcgtcgatc cgcgactgcg gcacggcagg gatgccgatg
     5581 ccgcgggcgg actgtgcgac gtaggacagc gccatcaccg acgcccgcgc gagctgctcg
     5641 cgggcctcgt cgtcggtgat gtcgagcagc ggtcggtagc cccagtacgg ggcaagcatc
     5701 gcgagggcgg cctgcacgtc gacgcggacg tcccccgtgt ggacagggat ggggaacgac
     5761 tcggcaggcg gcagacccgg gccgaaccgg ccgtcgacga gcagggccca gacgttgccg
     5821 aacgtcacct tgccgacgag gtcctcgatg tcgaccccgc gatagcgcag ggctccgccg
     5881 tccttgtccg gctcggcgat ctccgtgcgg aaggccacgt ggccctcgag tcctgacttg
     5941 aaacccggcg gtggcggggg tgcggcgtct tcgctcgggc ccgtcgtccc cgctgcggtc
     6001 gtgtcgccag cggtcgtgcc cgaagcagtt gtgcccaaag cagtcggcgc agtggtcact
     6061 gcgtcctcct catcgtggtc cccggacaag gtcaccggcc gcaggcaccg gtggcgtcgt
     6121 cggccgggtt ccacgtactg gctggagtgt cccgtccagc acgttaacca cggccgcccg
     6181 tgacggtcga cgtggctggc gtcaccatgc ccccagcgcc acgtggaggc aacgggacag
     6241 cagggtggga tcggtcaccg agcctatccc gacaaaccgt cgcggccata ccgtgatggg
     6301 tcggtaacgg gggaggcgcg tcatggcgga gcggctggcg acgatgcgtg ttgactacga
     6361 ctgcgagggg ttcgacatcg acgcgttggc cccgacgtgg cacgagcagc tcgcccgctg
     6421 gctcgtcgag gcggaggcgg ccggcgtggc cgaggccaac gcgatggttc tggccaccac
     6481 aggggaggac tctcggccct cgtcgcgcac cgtgctgtgc aagggcgtgg atgcgcgcgg
     6541 ggtggtcttc ttcaccaact acacctcggc caagagccac gacctccgcg tcagtcgcta
     6601 cgcctcggtc acctttccct ggtacgccct gcaccggcag gtgcacatcc gcggcccggt
     6661 cgagctcgtg accccggccg agactcagga gtactgggct agccggcctc ggggctctca
     6721 gctcggtgcg tgggcgtcgg cccagtcgac ggtcgtgcgt gaccgacggg ccctcgacga
     6781 cgcgctcgag gtcgtcagcc agcggttcgc ggccgagacg gtggtacccg ctccaccgca
     6841 ctggggtgga tggcggctca cgccggagca ggtggagttc tggcagggcc gcgagaaccg
     6901 catgcatgac cggctgcggt tcgaggtcga cggccacgaa cgcacctgga ccgtccgccg
     6961 tctggctcct tgaaccgggg tcgtgggcac tcagcgcccg actcgaggcg ggcctgcggg
     7021 ggtcgagatg gtaatcgtat tcccatgacg cccggtccgg aggaacctac ccgcccgaac
     7081 cggctcgtgc aggaaggccc gaccaggcag atcaacaccg ggcccgatgc gccgacccgg
     7141 ccaaccgggg ccgctccgac ctccgcgacc cgacgtatca cgggatccgt ccggggtgtg
     7201 cttgccgaca cccgcccgtt gcgtacgccg gcgttccggc ggctctggct ggcgggcgtg
     7261 atcacggtgg tcggcgcgca gctgtcggtg gtcgccgtcc cgacgcagat ctaccagctc
     7321 accggctcgt ccgcctacgt cggcctgacc ggactgttcg ggctcgtgcc gctggtcgtg
     7381 ttcgggctct ggggcggagc catcgccgac gtggtggacc ggcgcacggt gctcctcgcc
     7441 tcgggcggtg ccatcgcact gtcgtcgctg gcgctgtggg tcaccgccgc gagcggcgcc
     7501 ggtggggtgt gggccgtgct ggtgctgttc tccgtgcaga ccgccttcct cgccgtcaac
     7561 cagcccaccc gcagtgcagt gatccctcga ctgctgccgg tcgatcagct ccccgcggcc
     7621 aacgcgctca acatgaccgt ggtccagatc ggggccatcg ccgggccgct gctggccggc
     7681 gttctgatcc cggtggtggg gctgccgacg ctctacctcc tcgatgcagt cgcgctgctc
     7741 gcgacgctgt gggccacctg gcggctcccg gcggtgccgc cgggagcggc tatgacccgc
     7801 cgcgccggcc tgcgcgaggt gatcgacgga ttccgctacg tcgcgatgca caaggtgctg
     7861 ctcgtgtcgt tcctcatcga cgtcgtggcg atggggttcg gcatgccgcg tgtggtgttt
     7921 ccggagacgg cgcacacggt cttcggtgac cccgtctccg gtgggggagc gctcgggctg
     7981 ttgttcgccg caatcccgat cgggatggtg gtcgccgggc tgttctccgg acggctcacc
     8041 cgggtacggc ggcagggcgt ggcggtgacg ttcgccatct gcacgtgggg cctcggcgtc
     8101 gggttgttcg ggctgaccgg gtcgctgtgg ttcgcggtgc tgttcctcgc ggtcgcgggc
     8161 gcgggcgaca tggtcagctc gacgttccgc aactcgatgc tgcagacggt ggccaccgac
     8221 gagatgcgcg gccggatgca gggcgtcttc atcgtggtgg tggtgggcgg cccacggctg
     8281 gcggacctct ggcacgggcc ggccgcggac gcgatcggcc cctcggcggc cgcagcggtg
     8341 ggtggttttg cggtgatcgt gttcgcgatc gtcgtcgtcg ccgtgttccc catgttctgg
     8401 cgataccggg cgccggcggc cgccgccgca ccgccgaagg tggtgtgacg tccaggtctc
     8461 gatcgtgaga aaccggacaa caagcgggtg acggggcgcc cgccggccgt caccatcatc
     8521 acatgactga tgtgagcgcg gtcaccgcac agcctgagct gaagcgggcg atcggtccga
     8581 agctgctgct gttcttcgtg atcggcgaca tcctgggcac aggcatctat gccctcaccg
     8641 gttcggtggc cggggtgatc ggcggtgcgt tgtgggtgcc gttcgtgctg gcgttcatcg
     8701 tcgcgttcct cacggcgttc agctatctcg agctggtcgg caagtacccg cgtgcagcag
     8761 gtgcggcgct gtacacccac aaggcgttcg gcatcaactt cctgaccttc atggtcacgt
     8821 tcacggtgat ggcgtccggc gtcacctctg ccgccacggc ggccaaggcg ttcggcgaca
     8881 cctacctccg gcagttcatc gagctgccga cctgggcggt cgccgccgtc ttcatcgccg
     8941 gcctcgcgtt gatcaacttc cggggagtgg gggagtcggt caaggccaac gtcgtgctca
     9001 cgatcatcga gctgtccggc ctgctgctca tcatcgccgt cggcatctac gcggtggcgg
     9061 gtggcgcggg ggagccgtcg cgactggtgg agatcaacac tggggagacc accgcgttca
     9121 tcgcgatcac cgccgccgca tcgctggcgt tcttcgccat ggtcgggttc gaggactccg
     9181 tcaacatggc cgaggaatgc cacaacccgc cgcggatctt cccgcgcgcg atgctcctcg
     9241 gaatgggcgt cgctgccacg atctacgtga tcatcgcggt gctgtcctcg atgctcgtgc
     9301 cggccgccga ccttgccgcg gcgaagagcg gcgccctctt ccgtgtgctc gaggtggggg
     9361 caccggggtt cccgctgggc ctgttcgccg tgatcggcct gttcgcagtg gtcaactcgg
     9421 cgctgatcaa catgctgatg gcgagccgcc tgctctacgg gatgtcgaac gagcggatcc
     9481 tcccgcgggt gttcggcatg gtccacgagg ggcgccggac gccgtgggtc gcgatcgcct
     9541 tcaccagcgc catcgcgatc atcctggtgg cgagcgtcga catcaccgag ctgggtggca
     9601 ccactgcact gctcctgctg gccgtgttca ccatcgtcaa catcgcggtg ctcgtgctcc
     9661 gccgcgacaa ggtggaccac gaccacttcc gagcgccgac ctggagcccg tacgtcggca
     9721 tcgtgctctg cggcttcctc gcgacgccgc tgtccggacg gccgttgcag cagttcgtgc
     9781 tggctgggat cctgctcgcg gttggcgcag tgctctgggt catcaactat ctcgtcgtgg
     9841 gcagggtgga cgtggacccc gaacagctcg ccaagggctg agcgccccac gtcacatgct
     9901 ctggcgggcg cgctcgaccg gccgagcact agcgttgcgg ccaaccacgg aaaagaagag
     9961 tgcgagggaa cctccacatg gccgacgaca ccaagaacgc tgtcctccac caccccaacg
    10021 gcgagcacga gatggagatc caggctgcca ccgagggtgc gtccgcgttc gatatcagca
    10081 agctgctgcc tgcaacgggg ctggtcaccc tcgattccgg ctacggcaac accgctgcct
    10141 gtagctcggc gatcacctac atcgatggtg acgccggcat cctgcgctac cgcggctacc
    10201 cgatcgacca gctggccgag agctcgacct tcctggagac cagctaccta ctgatctacg
    10261 gggagctgcc gtcgaagtcc gagctcgagg ccttcaccaa caagatcagc cggcacaccc
    10321 tgctgcacga ggacctcaaa cggttcttcg acggcttccc gcgcgacgcg caccctatgc
    10381 cggtgctctc cagcgccgtg agcgcgctgt ccaccttcta ccaggacagc ctcgacccgt
    10441 tcgaccagga cgccgtcgaa ctgtcgacgg tccggctcct ggccaaggtg cccaccatcg
    10501 cggcctacgc gtacaagaag tcgatcgggc agccgttcct ctatccggac aactcactgg
    10561 gcctggtcga gaacttcctc cggatgacct tcggtttccc ggccgagccg tacgagatcg
    10621 accccgactt cgcccgggct ctcgacatgc tgctgatcct gcacgccgac cacgagcaga
    10681 attgctcgac gtccactgtt cggctggtcg ggtcgtcaca ggccaacctg ttcgcgagca
    10741 tctccgcggg catcaacgcg ttgttcggac cgctgcacgg cggcgccaac caggcggtgc
    10801 tggagatgct ccagcgcatc cgcgacgtgg aggacggcaa cgtcgacgcc ttcgtcgagc
    10861 gggtcaagaa gcgggagaag ggcgcccgcc tgatgggctt cgggcaccgc gtctacaaga
    10921 actacgaccc gcgcgccaag attgtcaagc agagcgccga cgagatcctc aagaagctcg
    10981 gggtcaccga ccagctgctc gacatcgcga aggcactcga ggagcgcgcg ctggccgacg
    11041 actacttcgt cgagcgcaag ctctacccga acgtcgactt ctacaccggc gtcatctacc
    11101 gggccatggg cttcccgacg aagatgttca ccgtcctctt cgcgctcggc aggttgcccg
    11161 gctggatcgc gcactggcgc gagatgatca atgaccccac cacgaagatc ggtcgcccgc
    11221 gccagctcta catcggctcg accgagcgct cgtacgtgcg tatgggcgac cgctgaggag
    11281 ttccgtaccg ccgcggggtg acatcgctga cgttcagccg tcagggatgc caccccgctg
    11341 acattccgcg acgtcgacgc gcgggatcag cccaccgcct ttaacgtcgg tccatggatg
    11401 acaccgcagt cgtctggttc cggcgtgacc tgcgggtact ggaccagccc accttcctcg
    11461 ccgctgcgga cgccgcgtcc cggtcactcg cgctgttcgt cctcgacccg gcgttgctcg
    11521 tgccgtccgg cagggcccgc cggacgttcc tctaccggtc attgcgcgcg ctcgacgaca
    11581 ctctggacgg gcggctgctc gtcgtccgtg gcgatccggc cgacgtcgtc ccgcgggtct
    11641 cggccggcat cgacgcggcc accgtgcacg tcgccgccga cttcggcccc tacggcacgc
    11701 gccgggacga ggcggtcgag aaggtgctcg cggacgc
//
LOCUS       DASRSC010000161        25431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4149311,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000161 DASRSC010000000
VERSION     DASRSC010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 25431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25431
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4149311"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2100
                     /locus_tag="VFE39_07770"
     CDS             <1..2100
                     /locus_tag="VFE39_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HET6258533.1"
                     /translation="TPRADSPAYRGRIERLAAQWAELGPPGDPGVAAHLATLLRKLGA
                     TLRAEPFWPLAAREIGAGLLATGLCGGPGRPTYRFDDVIAPSLRLLRHEAAAAIGMRG
                     PEGERRLAAVLDELVAGVARSLQELNGAEQRADARMPPVTDRHVRAVYEQSSFGMVIL
                     GLDGRVLDMNEAVCRMFGLDGPLDQPRPVSDFVHPDDVLDVVERFQRIVGGGPEVARM
                     ELRLVRPDSEVLWVHVTASRIVDDDGNPSHLMAVVEDVSDRHRLRSWLQEASCQDQLT
                     RLPNRAITEQWLHRAFTAEGPQRVGICVLDIDGFHAINDGLGQQTGDRLLLAVAGRLQ
                     LAAGDHLVSRVGADEFAVLMAEPESGADVGRLADRLQAALVIPFHVDGHTLVVSASIG
                     VAETPTAGGCAAELLRAADVARSWAKALGGGRRVVFDPERDAAESARFALVSGLRGAI
                     GRGEFRLAFQPLVQLADRRVRGAEALVRWQHPEEGLIGPGRFIELAEHSGAIVPLGRW
                     VLEEACGQAASWWRELGAAAPFVSVNVSPLQLAEPGWVHEVTGVLNATGLPPGMLQLE
                     ITEQAVLGDEAVTLDALNALRTAGVRLALDDFGTGYSSLAWLRRLPVHALKIDGSFID
                     GLRNPSADPTDFSIVRALVEMAHALGLEVTAEWVETAVQAERLAELGCDIGQGRWFGD
                     AGPGVWVPGLWRRSISS"
     gene            2127..3764
                     /gene="purF"
                     /locus_tag="VFE39_07775"
     CDS             2127..3764
                     /gene="purF"
                     /locus_tag="VFE39_07775"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET6258534.1"
                     /translation="MPSSPRNEHPTDRNPNGPDPTDGPALDPTAADPAPREECGVFGV
                     WAPGEDVANLTYYGLYALQHRGQEAAGIAVSDGRRMVVFKDLGLVSQVFDEQVLSSLK
                     GHLAVGHCRYSTTGSTTWENAQPTFRTTATGSGIALGHNGNLVNTAELRDEVVAARVS
                     PSKSDRTSIRSSTDSDLITELLAATAADIGVEEAAMRLLPRVRGAFSLVFADEQTLYA
                     ARDPHGVRPLVLGRLERGWVVASETAALDIVGASVVREVEPGELLAIDADGMRSSRFA
                     APEPKGCVFEYVYLARPDTAISGRSVHAARVEIGRRLAVEHPVDADLVIPVPESGTPA
                     AIGYAQGSGIPYGQGLVKNAYVGRTFIQPSQTIRQLGIRLKLNPLRDVIRGKRLVVVD
                     DSIVRGNTQRALVRMLREAGALEVHVRIASPPVRWPCFYGIDFATRAELVAGSLDIEG
                     VRRSIGSDSLGYVSLEGLIAATEQPRTRLCSACFDGEYPIPLPEEARLGKHLLEKLTV
                     PVEMADRAVAETVSGAASGDAGPLTIGYGAADALRRP"
     gene            3828..4922
                     /gene="purM"
                     /locus_tag="VFE39_07780"
     CDS             3828..4922
                     /gene="purM"
                     /locus_tag="VFE39_07780"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET6258535.1"
                     /translation="MPPTTSGAEGPGASYASAGVDIDAGERAVEALRPFAEKASRPEV
                     MGGIGGFAGLFALKMGRYTEPVLAASTDGVGTKIAVAQAVDKHDTIGLDLVAMVVDDL
                     VVCGAEPLFLQDYIAVGRVVPDQVATIVKGIAEGCQQAGCALLGGETAEHPGLMEDGG
                     YDISATGVGIVEADAMLRPDRVRPGDVLLAMGSSGLHSNGYSLARHVLLDIARIPLAG
                     HVEEFGHTLGEELLIPTRIYARDCLALTAETGVRTFAHITGGGLARNLERVLPPGLEA
                     VVERGTWTPAPVFNLIASRGRVERTEMEKTFNMGVGMVAVVPADDVDRALAVLTARHV
                     PAWVLGDVRRAGAEAPAAGRVHLKGDHPRF"
     gene            complement(4926..5615)
                     /locus_tag="VFE39_07785"
     CDS             complement(4926..5615)
                     /locus_tag="VFE39_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6258536.1"
                     /translation="MRGNLFAVAVDLVVLTVRDGRLCVLLVERPAPPFPGDLALPGGF
                     VRQREDLLDAATRELVEETGLAVGRHHLEQLGSYAAPDRDPRGRVLSVGHLALLPGLA
                     EPMGKAGTAIGTWYPVCEVGSLPFDHERIVADGVERARAKLEYTTLAASFCPPEFTVS
                     QLRGVYEAVWGTTIDPRNFQRKVTSTPSFLLPTGQKVRTGRGRPGQLFRRGDASALHP
                     PMLRDPEHARQ"
     gene            complement(6445..7446)
                     /locus_tag="VFE39_07790"
     CDS             complement(6445..7446)
                     /locus_tag="VFE39_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6258537.1"
                     /translation="MRLGLIGLGRIGAFHAQTLSELPGVGSLVITDAVPAVTAQVAAR
                     VGAEAVDSPAKLLAAGVDGVLIAAATDAHPELLLAAVEAGLPTFCEKPLARDAADALA
                     VAERVRATGVPVQIGYPRRFDPGFLAARAAVAGGELGWLHTVRSTTLDPAPPPGAYIA
                     ASGGIFRDCAVHDFDAVRWVTGREVVEVYATGSNRGEQFFTEVGDVDTAAALLTLDDG
                     TLAVISNSRYNARGYDVRLELHGSADSVAAGLDDGLPLRSTEPGVTFPAGPAHGFFMD
                     RLAAAFRAELAAFARVVAGELPSPCTVGDAMEVVWIAEAAALSLQEHRPVRIEEVRA"
     gene            complement(7455..8231)
                     /locus_tag="VFE39_07795"
     CDS             complement(7455..8231)
                     /locus_tag="VFE39_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6258538.1"
                     /translation="MGLLDDRVVLVSGGTQGVGAGVARAAAGEGATVAVSGRRREPGE
                     KLVAELREAGTAALFVPADVADVGQAQASVAAVVTEFGRVDALVTAAGLTSRGTLVDT
                     TPELFDAHIAVNLRGPFFLMQAAVRDMLSRGAPGSIVNVISSSELGGQPYLAPYVAAK
                     AGLAGLTRNAAHAHRFDRIRINGLDIGWTDTEGEDATQRTFHAAQDDWRERAGARLPM
                     GRLGRVEEVAAFVVFLLSDRSGVVTGSVIDWDQNVFGGLD"
     gene            complement(8231..9394)
                     /locus_tag="VFE39_07800"
     CDS             complement(8231..9394)
                     /locus_tag="VFE39_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6258539.1"
                     /translation="MSLLTGTGSRLTGDDCRIEDLKAVLDETTDRSRYPHSAAVEQGV
                     LIYDAENLRAATASEAGLWDVRAELVRALADGPGVVVLRGAFPDRTVVDRATAAFEEL
                     IAEQRASGAVAGDHFAPPGANDRVWNALEKLAMRAPEVFVDYYANDVLALVASAWLGP
                     GYQMTSQVNVVNPGGTAQVGHRDYHLGFLSNASIEQYPAHVHRLSPVLTLQGAVAHTD
                     MPVESGPTLYLPHSQKYEPGYLATNLPEFREFFETHHVQLPLRIGDAAFFTPAVIHGA
                     GHNRSTDMRRMANLLQVSSAFGRAMESVDRERMCLSLYPALRARGAAAAGPAIAAAAE
                     GYAFPTNLDRDPPVGGLAPPSQADLVRRALAESWDEDTFRIELAAHSARRRTA"
     gene            9512..10285
                     /locus_tag="VFE39_07805"
     CDS             9512..10285
                     /locus_tag="VFE39_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6258540.1"
                     /translation="MPARDAAPSGAPCWIDLFSSDPDRAAEFYGRIFGWNAEQTPAEY
                     GGYVNFTKNGVMIAGMMRNDGSAGQPDAWTTYLSTPDAKATTEAAAAAGGQVHLEPMQ
                     VMHLGSMGMVADPGGAAVGLWQPGEHKGYGLVGEAGTPVWHELHTRRYREVLDFYRTV
                     FGWETSTMGDTDDFRYSQMVVDGTGYAGVMDGSAFLPEGVPSTWQVYIGVEDVDAALQ
                     LAGELGGTVLEPAEDTPYGRLAKIADPTGAAIKMSSLPA"
     gene            complement(10355..10753)
                     /locus_tag="VFE39_07810"
                     /pseudo
     CDS             complement(10355..10753)
                     /locus_tag="VFE39_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            complement(11155..11370)
                     /locus_tag="VFE39_07815"
     CDS             complement(11155..11370)
                     /locus_tag="VFE39_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258541.1"
                     /translation="MTTLSPPVVYDPYSAAFQADPWPVYRHLRDTAPVYVSERWGFYA
                     LSRLDDVRATRPSCRSSASTSTTRPRT"
     gene            11480..12496
                     /locus_tag="VFE39_07820"
     CDS             11480..12496
                     /locus_tag="VFE39_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6258542.1"
                     /translation="MNPGRRPRLEDVAEDVGVSTASVSLVLRGVAGPSAETRKRVLAA
                     ATRLGYRPDRTASLLARRRTHLLGVPVVLRDAFRAELAEEVQVAADERGYSVALGAVT
                     PVHDEDRVVETLLDLRCEALLLLSPELRPGALAELGGRVPVVVVGRHVEPAGFDVVRV
                     ADDEGVGQAVDHLVGLGHRDLVHVDGAGAPMAADRRSGFLAATRRHGLDSARVLRGGY
                     DEAAGAAAARALLDEPVLPTAVLAANDRSAIGLLDVFLRGGVRVPGDVSVVGYDDSGL
                     AGLAHVDLTTVSQEAAAQAARAVEAAVERLDGGRSEPREVVLPPRLVVRGTTGPPRQA
                     GRGG"
     gene            12658..13413
                     /locus_tag="VFE39_07825"
     CDS             12658..13413
                     /locus_tag="VFE39_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6258543.1"
                     /translation="MVLVQQSRSAPPRDVVVLAEVSKSYPGRAAVRALDRVTVGFASG
                     SFTAVMGPSGSGKSTLLHCAAGLDRPSEGRVLLAGQDISALREPKLTVVRRAHVGFVF
                     QSFNLVDALSVWDNVLLPQRLAGKRPDEAWAREVLARVGLAGREKARPGELSGGQQQR
                     VALARALAGRPEVIFGDEPTGALDLSTGREVLALLRRFVDEFGATVVMVTHDPAAAAW
                     ADRVVFLADGRLAGDLISPTAAQVSTRMVELTR"
     gene            13410..15956
                     /locus_tag="VFE39_07830"
     CDS             13410..15956
                     /locus_tag="VFE39_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6258544.1"
                     /translation="MIIKLAAGTVRRRRGAFAGTFLTAFLAVALIAGSGLLLFSVLTA
                     GPGADRFAAADAVVAGDRAVVLTTEQDKGDGEVKVKTKSERLTGAPALPAELVTAVAG
                     TPGVATAVPDAAFPVAVDLGGVPVRAPDRAAVVAHGWASAALTPYELTQGAPPGPGGV
                     VLDAALADGLRIGEAVRVTTRTGARDLRLVGVAAPATAEGVPGQAAVFVADAQVGELS
                     GLAGPTAVGVRVAPGADAAAVVAGLQQRIGGAAAVHTGADKVTADLPGALPDYIGAIS
                     IFGFVLGITGFAAVFVLTGTVSLAIRQRLRELALLRTAGATPRQLRRMLSLEIAIVTL
                     VAAAPALPVGVVVAHLVAARFRDLGAVPPQFTVALNVIVLIAAVVVGLLVTLLSTAVA
                     AHRAVRIAPTQALRETVTASGGGRVLRVVVAAVLVAGAVAVLGLVPLGGVYGMGMGFV
                     SSALLLCAVAALGPLAVGAVTAVVSRVVALSSVLGRIAAVMTRAESRRVVGVAIPLTL
                     MFAINATMLIDGEILTRVTADQQAARTASATVRVAATNAPGLPLDVAGQVCELPAVTG
                     SAATIGTRVIVDLGGKPEDYPAQGLQVSGEQALDLGVTAGSLDDLGRDGPDQATVAVS
                     GPLAEERGWKIGDRPDFWLADGTRTPLTVVAVFENWRGFGDLVLPAELAARHDPRGLV
                     GALYLRGEPGVAEAVHAEWPALAATDARDPRGGTADVQNQQAAWELMVVISIGFTAIA
                     VVNTFAIATSARRSEFAGLRLIGATAPQLHRLLGLEALVTVAVALVLGCLITGGVVGT
                     FSVAQDGQWRLFADPLRYLAMIGGVGFLGLVAAAVPARLVVRRRSLPGQA"
     gene            15987..17222
                     /locus_tag="VFE39_07835"
     CDS             15987..17222
                     /locus_tag="VFE39_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HET6258545.1"
                     /translation="MIVRAARELAYLLAGVLIGPATFLWALITTLASGLLSVTYLGPP
                     VFLVLTWVTRRLAGLERWRAGALLGRPVPAPYEPASGANPWARGRTLTREPATWRDLT
                     WLVLMFPLGLATGIPGVVVAVVDLGTILAPLWLWAVPNPHLPPVVDTLFNTVPGRFAL
                     TLLGVLLALPVRWLVGTLARTQAELAVKLLGQGPRQRLADRATELAVTRRRVVDAQAA
                     ELQRIERDLHDGAQARIVAAGMTLALAERKLRATGPQGDPARDDLLTARRQLDEALAE
                     LRRLVRGIYPPILTDRGLVAALGALAGDASLPVVVRAGDLGELAPAVQAAAYFVVAEA
                     LANTAKHATADECVVDVVRREDVLSLEVRDDGRGGADPSGSGLTGLRRRVEALDGRME
                     VTSPPGGPTVVRAELPCAS"
     gene            17210..17854
                     /locus_tag="VFE39_07840"
     CDS             17210..17854
                     /locus_tag="VFE39_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6258546.1"
                     /translation="MRIVIAEDLLLLRDGLIRILTDTGHTVVAAVDNAPDLESAVREH
                     RPDLSIVDVRLPPGFRDEGLRAALSVRGREPRTPILILSQYVERTYAAELLADGNGAV
                     GYLLKDRVTALDEFLDAIERVAGGGTVMDPEVVRQLFARNNRDQGVSALTPREREVLA
                     LVAQGLSNSAICTELVLAPPSVEKHITNILTKLELPPADDTHRRVRAVLAYLNH"
     gene            complement(17866..18099)
                     /locus_tag="VFE39_07845"
     CDS             complement(17866..18099)
                     /locus_tag="VFE39_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258547.1"
                     /translation="MTARGDLLGPAPHPSGKLTIVINISVSSRSLAAARSVDPDVSAA
                     AVRAAVEVAIAEQMRELRRSRDATRLDPTPYDG"
     gene            complement(18171..19043)
                     /locus_tag="VFE39_07850"
     CDS             complement(18171..19043)
                     /locus_tag="VFE39_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET6258548.1"
                     /translation="MNDQRIAGAPISWGVCEVPGWGYQLPLARVLAEMREVGLAATEL
                     GPDGFLPGEPGAMAEVLSRHHLQAVGGFTPLLLHLADRDPVPEVEKLLQAYAATGSDV
                     LVLSAVTGLAGYDARPALDDAGWRTLLTNLDRLTGLAIEHGVRAVLHPHIGTLVENGE
                     EVQRVLDGSSISLCLDTGHLLIGGTDPAALTREAPHRIAHTHLKDVDVTLAHQVQAGR
                     RTYTEAVREGMYRPLGQGDVDFEAIVGHLRGQGYDGWYVLEQDTILTEEPRGEGPLAD
                     IRVSVEHLRSVLGS"
     gene            complement(19036..20085)
                     /locus_tag="VFE39_07855"
     CDS             complement(19036..20085)
                     /locus_tag="VFE39_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6258549.1"
                     /translation="MSRRVAVGLAGLGRMGRIHARNLVSRCASARLACVYDADTAVAR
                     QLAEELEVPWTASFDELLADDGVKAVAIATSTGSHAELAMAAAAAGKHIFCEKPISLD
                     RDTTLAVIDAVGQAGVVLQVGFHRRSDPDWVAAAQRIRAGELGAVTLFRTSLRDMTSP
                     PPEYLTGSGGFFVDVTVHDLDVARWLVGEVVEVSAHGAATDPAFAALGDIDTAVVVLR
                     FENGALGVIDNSRSARYGYECSTEVMGSAATVRIDAPQRRHYEWRTPGLASHELPRDF
                     EERYPDAYADELDAFGRCVRDGGAPRVTGHDALAAFDLARAARRSWESGRPVGVKPLR
                     TANGVLYELEELQSE"
     gene            complement(20082..20951)
                     /locus_tag="VFE39_07860"
     CDS             complement(20082..20951)
                     /locus_tag="VFE39_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET6258550.1"
                     /translation="MKLAVDPYMLRRTPLLELPALVAEFGYQHIELSPREDFLPFFVH
                     PRADRARITAFRKELAGAGVEIASVLPLYKWSGPDEDERQAAVRYWRRAIQITVDLGC
                     GVMNSEFNGRPEQAAASEAQFWRSMEELLPVFEREGVRLVLEPHPDDFMEDGRGAVDL
                     IRGIDNELVTFLYCAPHTFHQGGDLAGVIRYAGPLLTQLHIADSFDHTGSSGLRYIVN
                     PPGSTARIHQHLDVGQGEVNWDLFFATLAEVGFDGIATVCVFAWEERARESLIFNREK
                     VQEYLGRHAVGSA"
     gene            complement(20948..21967)
                     /locus_tag="VFE39_07865"
     CDS             complement(20948..21967)
                     /locus_tag="VFE39_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6258551.1"
                     /translation="MTLGVGVIGVGMIGQDHIRRLTHVLSGARVAAVTDVDLDRARTV
                     ADDLPAAVLHTSGQDLIADPDVDAVVVTSWGPTHEEYVLACIAAGKQVFCEKPLATTE
                     EACARIVDAEVAAGRRLVMVGFMRRYDAQYRAMKDVVTGREIGLPLLMHAAHRNPSVP
                     SHFTSDMIINDSTVHDIDVARWMFDDEIDAITVFTPRVSRKAAPGFNDPLLVLLEMRS
                     GVLVDVEAMVNAGFGYDIRGEVVCEDGTVELSESAGALVKSAGRIAGRVPADWRERFV
                     RAFDTEFQAWIDAVTAGGTTGPSAWDGYAATVACETGLAALHSGTRVPVVLRERPDLY
                     KEAAR"
     gene            complement(21999..22814)
                     /locus_tag="VFE39_07870"
     CDS             complement(21999..22814)
                     /locus_tag="VFE39_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6258552.1"
                     /translation="MTDTIAEHAAQDLQAGTPLVQMENVGKAYGAIRALEEINLTVNA
                     GEVTCVLGDNGAGKSTLIKIISGLHPHTEGVVKVDGEQVHFASPRESLDHGIATVYQD
                     LAVVSLMEVWRNFFLGSELRKGTYPLAPLDITGMRRIADSELRKMGIEVKDINQPIGT
                     LSGGQRQCVAIARAVYFGARVLILDEPTAALGVKQSGVVLKYTAAARDAGLGVVFITH
                     NPHHAYMVGDHFIILKLGRCVLDKKRAGVTLDELTAEMAGGQELDELSHELKR"
     gene            complement(22822..23886)
                     /locus_tag="VFE39_07875"
     CDS             complement(22822..23886)
                     /locus_tag="VFE39_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6258553.1"
                     /translation="MSSTVTTSPPPQSPSAQQPARVRRSLVSRVLGRPEIGALAAAIA
                     IFIFFFAVAAPFRSLPAFSTVLYASSTIGIVAVGVALLMIGGEFDLSAGVAVATAALA
                     SAMISYQFSLNAWVGVVISLVIALAIGFVNGYLVVRTGIPSFLITLSTFFVLQGVNLG
                     VTKLVTGTVATTNIDRLDGFTSARAFFAGNIDLGFMTLEITVLWWILFVAVATWVLLR
                     TRTGNWIFASGGNAASARAVGVPVPKVKIGLFMGVAFLAWFLGQHLLFNFSSVQAGLG
                     VGNEFIYIIAAVVGGALLTGGYGSAVGSALGAFIFGMVTQGIVFAGWDPNWFKAFLGA
                     MLLLAVLVNLYVKNYASQRR"
     gene            complement(23948..24922)
                     /locus_tag="VFE39_07880"
     CDS             complement(23948..24922)
                     /locus_tag="VFE39_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6258554.1"
                     /translation="MRALAVAAALGVALAACSSQGGANQANQNSGPAVGSAGGKQYTI
                     AMVTHEAPGDSFWDKVRAGAQQAAKTLNVDLKYSNSNDAGQQATLVQNAIDSKVDGIA
                     VTLAYADQVGPAAQKAVQAGIPTVAFNSGINEYTKYGIPMYFGSDEDLAGQSAGQRMA
                     AAGGRKAICVVQEQGSVALEARCAGVKKTFPNTENLQVNGTDLPSVQQTIGAKLQQDP
                     SITDVVTLGAPIALAALQAKQGTSSQAKVVTFDLNADVAKDIQDGTIDFSIDQQPYVQ
                     GYMAVQALWLNLSNGNDIGGGKPVLTGPSFVDKSNIDKIATYAANNTR"
     gene            complement(24994..>25431)
                     /locus_tag="VFE39_07885"
     CDS             complement(24994..>25431)
                     /locus_tag="VFE39_07885"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET6258555.1"
                     /translation="DTHVDTQERVERFLEDTDPEYVDLCLDTGHISYCDGDNLAIIRR
                     FPERIRYVHLKQVDPEIRRRVREQRLSLAEAVPLGVMVEPPYGVPEMPPLLDALAALD
                     TDLYTVVEQDLYPVEPQIPLPIGARTAGYFRGCGLGPVRRWPY"
BASE COUNT         3448 a         8872 c         9318 g         3793 t
ORIGIN      
        1 actcctcgag cagactcccc ggcgtaccgc ggccgcatcg aacggctcgc cgcccagtgg
       61 gccgagctgg gtccgccggg cgaccccggc gtggccgctc acctcgccac tctgctccgc
      121 aagctcgggg cgaccctgcg agccgagccg ttctggccgc tcgccgcgag ggagatcggt
      181 gccgggctgc tcgccaccgg gctgtgcggc ggccctggcc ggccgaccta ccggttcgac
      241 gacgtgatcg cacccagcct ccgccttttg cggcacgagg cggcagcggc gatcgggatg
      301 cgcggtcccg agggcgagcg gcggctcgct gccgtgctcg acgaactggt ggcaggcgtc
      361 gcccgttcgc tgcaggagtt gaacggtgcc gaacagcggg ccgatgcccg gatgccgccg
      421 gtcacggatc ggcacgtccg cgcggtgtac gagcagtcgt ccttcggcat ggtgatctta
      481 ggcctggacg gtcgcgtcct cgacatgaac gaggccgtct gtcgcatgtt cgggctggac
      541 gggccactgg accagccacg gccggtgtcc gacttcgtcc accccgacga cgtgctcgat
      601 gtcgtcgagc gcttccagcg catcgtcggc ggcgggcccg aggtcgcgcg gatggagctg
      661 cggctcgtcc ggcccgactc ggaggtgctc tgggttcatg tcaccgcatc gcgcatcgtc
      721 gacgacgacg gcaacccgtc acacctgatg gcggtggtgg aggacgtctc cgaccggcat
      781 cggctgcggt cctggctcca ggaggcgtcc tgccaggacc agctcacccg cctgccgaac
      841 cgcgccatca ccgagcagtg gctgcaccgc gcgttcaccg cagagggacc ccagcgggtc
      901 ggcatctgcg tcctcgacat agacggcttc cacgccatca acgacggcct cggtcagcag
      961 acgggcgatc ggctgctcct cgccgtggcg ggcagactgc agctggccgc gggcgaccat
     1021 ctcgtcagcc gcgtcggagc ggacgagttc gcggtgctga tggccgaacc ggagagcggc
     1081 gccgacgtcg gcaggctcgc cgaccggctc caggccgccc tggtgatccc gttccatgtc
     1141 gacggtcaca ccctggtggt ctcggcgagc atcggggtgg ccgagacgcc caccgccggg
     1201 gggtgcgccg ccgagctcct acgggccgcc gacgtcgccc ggtcgtgggc caaggccctc
     1261 gggggtgggc gcagggtggt gttcgacccc gagcgtgacg cggcggagtc ggcgcggttc
     1321 gcgctggttt cgggcctccg gggcgcgatc gggcgcggtg agttccggct ggcattccag
     1381 ccgctcgtcc agctcgccga ccggcgggtc cggggcgcag aggcgctggt ccgctggcag
     1441 caccccgagg aggggctgat cgggccgggc cggttcatcg agctggccga gcacagcggc
     1501 gccatcgtcc cgctcggtcg atgggtgctc gaggaggcct gcgggcaggc ggcgagctgg
     1561 tggcgcgagc tcggcgccgc ggcgccgttc gtcagcgtga acgtctcgcc gctgcagctg
     1621 gcggagccgg gctgggtcca cgaggtcact ggcgtgctga acgccaccgg gttgccgccc
     1681 ggcatgttgc agctggagat caccgagcag gcggtgctcg gcgacgaggc ggtcaccctc
     1741 gacgcgctca acgcgctgcg gaccgcgggc gtccggctcg cgctcgatga cttcggcacc
     1801 ggctactcaa gcctcgcctg gctgcggagg cttccggtgc acgcgctcaa gatcgacggc
     1861 tcgttcatcg acgggctgcg caacccctca gccgatccga cggacttctc gatagtccgg
     1921 gcgctggtcg agatggccca cgcgctcggc ctcgaggtga ctgccgaatg ggtggaaacg
     1981 gccgtgcagg cggaacggct ggccgagctc ggatgcgaca tcgggcaggg caggtggttc
     2041 ggtgacgcag gtcccggagt gtgggtcccg ggtctctggc ggcgtagcat cagttcttga
     2101 gtcgtcgtct gcgcaaggag cgtgttgtgc cgagttcccc ccggaacgag cacccgaccg
     2161 accgcaatcc gaatggtccc gaccccaccg acggccccgc actcgacccc accgctgctg
     2221 accccgctcc tcgcgaggag tgcggggtct tcggtgtctg ggcccccggc gaggatgtcg
     2281 ccaacctcac ctactacggg ctctatgcgt tgcagcaccg tggccaggaa gcggccggaa
     2341 tcgccgtgtc cgacggccgc cgcatggtcg tcttcaagga cctcgggctg gtcagccagg
     2401 tcttcgacga gcaggtgctg tcgtcgctga agggtcacct tgcagtcggg cactgccggt
     2461 actcgaccac cggttccacc acgtgggaga acgcccagcc gaccttccgc accaccgcca
     2521 ccggcagcgg tattgcgctg ggacacaacg gcaacctggt caacacggcc gagctgcgcg
     2581 acgaggtcgt tgcggcgcgg gtctccccgt ccaagtccga ccgcacctcg atccgctcca
     2641 gcaccgactc ggacctgatc accgagctgc tggccgccac cgccgccgac atcggcgtcg
     2701 aggaggcggc gatgcgcctg ctgccccgcg tgcgcggtgc gttctcgctg gtgttcgccg
     2761 acgagcagac gctctacgcc gcccgcgacc cgcacggggt gcgcccgctg gtgctcggcc
     2821 gcctcgaacg cgggtgggtg gtggccagtg agaccgcggc cctggacatc gtcggtgcgt
     2881 ccgtggtgcg tgaagtcgag ccgggcgagc tgctggcgat cgacgccgac gggatgcgca
     2941 gctcccggtt cgcggcacca gagcccaagg ggtgcgtgtt cgagtacgtc tacctggccc
     3001 ggccggacac cgcgatctcc gggcggtccg tgcacgcggc ccgggtggag atcggccggc
     3061 gactcgccgt cgagcacccc gtcgatgccg acctggtgat ccctgtaccc gagtcgggca
     3121 cgccggctgc catcggctac gcgcagggct ccggcatccc ctatggacag ggcctggtga
     3181 agaacgccta cgtcgggcgc accttcatcc agcccagcca gacgatccgt cagctcggta
     3241 tccggctcaa gctcaacccg ctgcgcgacg tcatccgcgg caagcggctc gtggtcgtcg
     3301 acgactcgat cgtgcgcggc aacacccagc gcgcgctcgt acgcatgctg cgggaggccg
     3361 gtgcgctcga ggtccacgtg cggatcgcgt cgcctccggt gaggtggccc tgcttctacg
     3421 gcatcgactt cgccacccgg gccgagctgg tggcgggctc gctggacatc gagggtgtcc
     3481 gccggtcgat cggctcggac agcctgggat acgtctcgct ggaggggctg atcgccgcca
     3541 ctgagcagcc gcgtacccgg ctctgctcgg cctgcttcga cggcgagtac ccgatcccgc
     3601 tgcccgagga ggcgcggctc ggcaagcacc tgctcgagaa gctcacggtg ccggtggaga
     3661 tggccgacag ggctgtggcc gagaccgttt ccggcgccgc ctcgggcgat gccggcccgt
     3721 tgacgatcgg ttacggtgcc gccgacgcgc tgcgccgtcc ctgaccctca gtcgacctgt
     3781 acccggggcg cgccccgcaa actacacacg ccaggagctt tgcgacaatg ccgccgacca
     3841 cctccggagc cgaaggtccg ggcgccagtt atgcgtccgc cggcgtcgac atcgacgcgg
     3901 gtgagcgcgc ggtcgaggct ctgcgcccgt tcgcggagaa ggcgagccga ccggaggtga
     3961 tgggcggcat cggcgggttc gccgggctgt tcgcgctgaa gatgggccgc tacaccgagc
     4021 ccgtgctcgc ggcgtcgacg gacggggtgg gcacgaagat cgccgttgca caggccgtcg
     4081 acaagcacga cacgataggc ctcgacctcg tggccatggt cgtcgacgac ctcgtggtgt
     4141 gcggtgccga gccgctgttc ctgcaggact acatcgccgt cggacgagtc gttccggatc
     4201 aggtcgccac catcgtcaag ggcatcgccg agggttgcca gcaggccggc tgtgcgctgc
     4261 tgggtgggga gaccgccgag caccccgggc tgatggagga cggtggctac gacatctccg
     4321 ccacgggcgt cggaatcgtc gaggccgacg cgatgctgcg gcccgaccgg gtgcgccccg
     4381 gcgacgtgct tctggccatg ggctcgtccg gcctccactc gaacggctac tcgctggcca
     4441 ggcacgtgct gctggacatt gcgcggatac cgctggccgg tcatgtggag gagttcgggc
     4501 acaccctcgg cgaggagctg ttgatcccca cccggatcta cgcccgcgac tgcctcgcgc
     4561 tgaccgccga gaccggcgtt cgcaccttcg cgcacatcac cggcggtggc ttggcccgca
     4621 atctggagcg cgtgcttccg cccgggctcg aggccgtggt ggagcgcgga acctggacac
     4681 ccgcaccggt gttcaacctg atcgcctccc gcggccgggt cgagcgcacg gagatggaga
     4741 agaccttcaa catgggcgtc gggatggtgg ccgtggtgcc tgccgatgat gtcgaccggg
     4801 ccctggcggt gctcaccgcc cggcacgtcc cggcatgggt gctcggtgac gtccgacggg
     4861 cgggtgcgga ggcaccggca gcgggccggg tgcacctgaa gggtgaccac ccccgcttct
     4921 gaccgctact ggcgcgcgtg ctcagggtcg cggagcatcg gaggatgcag agcgctcgca
     4981 tcccctcgcc ggaacagctg gccagggcgg ccccggccgg tgcggacctt ctggccggtg
     5041 ggtagcagga agctcggcgt ggacgtgacc ttgcgctgga agttgcgcgg gtcgatggtc
     5101 gtcccccaga cggcctcgta caccccgcgg agctgcgaca cggtgaactc gggagggcag
     5161 aacgacgcgg ccagcgtcgt gtactccagc ttcgcgcggg cacgttcgac accgtcggcg
     5221 acgatccgct cgtggtcgaa cgggagtgaa ccgacctcgc acaccgggta ccacgtcccg
     5281 atcgccgtcc cggccttgcc catgggctcg gccaggccgg gaaggagggc gagatggccg
     5341 acgctcagca cccggccgcg ggggtcgcga tccggagcgg cgtagctgcc gagctgctcg
     5401 agatggtgcc ggccgaccgc caggcccgtc tcctcgacga gctcgcgcgt ggcggcgtca
     5461 agcagatcct cacgctgccg cacgaacccg cccggcaacg ccaggtcacc cgggaacggg
     5521 ggcgccgggc gctccacgag cagcacacag agccggccgt cgcgcacggt gaggaccacc
     5581 aggtccacgg cgacagcaaa gaggttgcca cgcatcggat ctcatcttgt cgagccagat
     5641 aggcggatca tcgtcgggct gacgctacag gtgacggtct gatcgatgaa cggggtgttg
     5701 atcgtcgtgg ggcagctgct gcggttggga ccgcatctcg tggaggtgcc gatcagcgcg
     5761 atgctcgtgc tcggggtggg gttcgtggca ggcgccgaga gcgcggcgcg cgctggagac
     5821 gctcgtcggg gcgaccgtgg gtgtgctggt gaacctcgtc ttcccgcccg cggtgcaggt
     5881 gcgctccgcg gcggcggcgg tgtagcggct atccgaggag atcgccgcgc tgctcgacgc
     5941 cgcgggggat ctcgccgcgg cccggcagcc ggccggccac cgctcaaggc cgttcgttcg
     6001 ttgagctcgc ttcccgatgt tctcgcgctc tgcaccgacg cgggcgatcg cgggcccggc
     6061 cgggtggcgc ggccgcgggc gaggacgggg cgatgtcaag gcgtcggcat cgttggggtg
     6121 ttcctctccg ggatcgagca tggtcaccag gagaggtagc acccccgatc gcggagtacg
     6181 tgataactcg ctgtatcacg taccgatccg ggcgagggtg cgcttccagt ggtcctctgc
     6241 gatctcgaag atccccatgc atgccggtgt cgataccgcc ttccgccatg ctcgcgtcct
     6301 cttcgcctgg gtcgttaggg gcgatgagga cggggaaccg agtgagcggt atgccgttcc
     6361 ggccccgggt cctgcgcgtg tccacggcgg ggcgggagcg actcgcgggc gcgaagcgag
     6421 cgactcgcgg ggtgggccgg agcgtcaggc gcggacctcc tcgatgcgca cgggccggtg
     6481 ctcctgcaag gacagcgcgg cggcctcggc gatccagacg acctccatgg cgtccccgac
     6541 ggtgcacgga gaaggcagct ctcccgcgac cacacgcgcg aatgcggcga gctccgcgcg
     6601 gaaggcggcc gccaggcgat ccatgaagaa cccgtgcgcc ggcccggccg ggaacgtgac
     6661 gcctggttct gtcgagcgca gcgggagccc gtcgtccagc ccggcggcga cgctgtcggc
     6721 cgagccgtgc agctccagcc gcacgtcgta gccccgcgcg ttgtaccggc tgttggagat
     6781 cacggcgagc gtgccgtcgt cgagggtcag cagggcagcc gcggtgtcga cgtcgccgac
     6841 ctcggtgaag aactgctcac cccggttgga gccggtcgcg tagacctcga cgacctcgcg
     6901 tccggtcacc cagcggaccg cgtcgaagtc gtgcacggcg cagtctcgga agatcccgcc
     6961 ggaggccgcg atgtaggccc ccggtggcgg tgccggatcc agcgtggtcg agcggacggt
     7021 gtgcagccat ccgagctcac cgccggccac cgcggcgcgg gccgccagga acccggggtc
     7081 gaatcggcgc gggtagccga tctggaccgg gacgccggtc gcgcgcaccc gctcggccac
     7141 agcgagggcg tcggcggcgt cgcgggcgag cggcttctcg cagaaggtcg gcagcccggc
     7201 ctccaccgca gcgaggagca gttcggggtg tgcgtcggtg gccgccgcga tcaggacgcc
     7261 gtcgaccccg gccgcgagca gcttcgccgg ggagtcgacc gcttccgccc ccactcgtgc
     7321 ggcgacctgc gcggtgacgg cgggcacggc gtcggtgatc acgagcgagc cgacgccggg
     7381 cagctcgctg agcgtctgtg cgtggaaggc gccgatccgg ccgaggccga tgagtcccag
     7441 acgcagagaa tcgttcaatc cagccctccg aagacgttct ggtcccagtc gatgacggag
     7501 ccggtgacga cgccgctgcg gtcggacagc aggaacacga cgaaggcggc gacctcctcg
     7561 acccgtccga gccggcccat cggcaaccgc gcgcccgcgc gctcgcgcca gtcgtcctgc
     7621 gcggcatgga aggtgcgctg tgtcgcgtcc tcgccctcag tgtcggtcca gccgatgtcg
     7681 aggccgttga tccggatccg gtcgaagcgg tgggcgtggg cggcgttgcg ggtgagtccg
     7741 gcgagcccgg ccttggcggc gacgtacggc gcgaggtagg gctgtccgcc cagctccgac
     7801 gaggagatca cgttcacgat cgagccgggc gcgccgcggg acagcatgtc gcgtacggcc
     7861 gcctgcatga ggaagaacgg gccgcgcagg ttgacggcga tgtgggcgtc gaagagttcg
     7921 ggcgtggtgt cgacgagggt gccgcgcgag gtcagcccgg ccgcggtgac cagggcgtcc
     7981 acccggccga actccgtcac caccgcggcg acagacgcct gcgcctgccc gacgtccgcc
     8041 acgtccgcgg gaacgaacag cgcggcggtt cctgcctccc tcagctcggc caccagcttc
     8101 tcgccgggct cgcgacgcct gccggagacg gccaccgtgg cgccttcgcc ggcggccgcc
     8161 cgcgccacgc cggcccccac cccctgcgtg ccgccgctga ccagcacgac tcggtcgtcc
     8221 agaagcccca tcacgccgtc ctgcgccggg cggaatgggc ggcgagctcg atccggaacg
     8281 tgtcctcgtc ccagctctcg gcgagcgccc ggcggaccag gtcggcctgg ctgggcgggg
     8341 cgagcccgcc gacgggtggg tcacgatcca ggttcgtggg gaacgcgtac ccctcggcgg
     8401 ccgcagcgat cgccgggcct gcggcggccg caccacgggc gcgtagcgcc gggtagagcg
     8461 acaggcacat ccgctcgcgg tcgacgctct ccatcgcacg cccgaacgcc gaggagacct
     8521 gcagcaggtt ggccatccgc cgcatgtccg tggaccggtt gtgcccggcg ccgtggatca
     8581 cggccggggt gaagaacgcc gcgtcgccga tccgcagcgg cagctggaca tggtgggtct
     8641 cgaagaactc ccggaactcc ggcaggttcg tggcgaggta gcccggctcg tacttctgcg
     8701 agtgcggcag gtacagggtc ggcccgctct ccacgggcat gtcggtgtgc gccaccgcgc
     8761 cctgcagcgt cagcacgggg gagagccggt gcacgtgcgc cgggtactgc tcgatcgacg
     8821 cgttcgagag gaaccccagg tggtagtcgc ggtggcccac ctgtgcggtc ccgccggggt
     8881 tcaccacgtt gacctgcgag gtcatctggt agcccgggcc gagccaggcc gaggcgacca
     8941 gcgcgagcac gtcgttcgcg tagtagtcga cgaacacctc cggtgcgcgc atcgcgagct
     9001 tctccagggc gttccacacg cggtcgttcg cgcccggtgg cgcgaagtgg tcgcctgcga
     9061 cggcacccga agcccgttgc tcggcgatca gctcctcgaa cgccgcggtg gcgcggtcga
     9121 cgaccgttcg gtccgggaac gcgccgcgga gcaccacgac acccggcccg tcggcgagcg
     9181 ctcgcaccag ctcggcccgg acatcccaca ggccggcctc cgacgcagtc gcggcccgca
     9241 ggttttcggc gtcgtagatc agtactccct gctcgacggc agcagagtgc gggtaccggg
     9301 accggtcggt ggtctcgtcc aggacggctt tcaggtcctc gatccggcag tcgtccccgg
     9361 tcagccggct gccggtaccg gtcagaagcg acatactcgt ccctttcctc gttccatgtg
     9421 gtgctccgag ttcgcccgat gagttattgg tgttcccctg gtcctattcg tcgtgggaac
     9481 ctgccccacc ccgacaccca ggaggccccc cgtgcccgct cgtgatgccg ctccgtccgg
     9541 agcgccctgc tggatcgacc tgttcagctc cgaccccgac cgcgccgccg agttctacgg
     9601 ccggatcttc ggctggaacg cggagcagac cccggcggag tacggcggct acgtcaactt
     9661 cacgaagaac ggcgtgatga tcgccgggat gatgcgcaac gacgggtcgg ccgggcagcc
     9721 cgacgcctgg acgacctacc tgtccacgcc cgacgccaag gccaccacgg aggcggccgc
     9781 cgcggcgggc gggcaggtgc acctcgagcc gatgcaggtg atgcacctgg gctcgatggg
     9841 catggtggcc gaccctggcg gcgccgcggt cgggctctgg cagcccggtg agcacaaggg
     9901 ctacggcctg gtcggcgagg caggcacgcc ggtctggcac gagctgcata cccggcgcta
     9961 ccgggaggtg ctcgacttct accgcacggt gttcggctgg gagaccagca cgatgggcga
    10021 caccgacgac ttccgctact cgcagatggt ggtcgacggg acgggctacg cgggtgtcat
    10081 ggacggtagc gcgttcctgc ccgagggtgt gccgtccacc tggcaggtct acatcggcgt
    10141 cgaggacgtc gacgccgctc tgcagctggc cggcgagctg ggcggcacgg tgctggagcc
    10201 cgccgaggac acgccgtacg gccgcctggc gaagatcgcc gacccgacgg gcgcggcgat
    10261 caagatgtcc tccctccccg cgtagtccgc cccgtccgtc gagagccgca ttacccaccg
    10321 ttatccggcg gcggccagga gggtctgcgc ggaggccgct ctgcgccagc tcgacctcga
    10381 acgatccggc gagcgtcggc tcggcgagcg gcttcggcgc gaagcgctcc acctgcagcg
    10441 accccggcgg ccagctcgcg cagagctcct cgacggcggc gagcagcggc cccggaccgc
    10501 agcagtacac gagcgtgtcc gggcggggag cgccgagcag gtcgtcgagg tcgagcaggc
    10561 cggtctcgtc ctgcggacgg atggtgacgc gggggtcggt cagctcgtcg aggaacgcca
    10621 tcgacgcccg cctccgcccg ccgtagacca gctcccactc cgcgccggcg gcatcggccg
    10681 ccccgatcat cgtcaggatc ggagtgatgc cgacgccgcc cgcgatgaac agataccgcg
    10741 gcggtggcag cagcgggcag gcgacctgca gcggggcgtc gaaccgcagc gcctcctcca
    10801 cggcggcggg gatccgtgac gggtcggcca gcagcgcggc ccgctggtcc gggcgggggt
    10861 cgtcgttctc ccggtccttg agcccgtgtg accagcgcac gaggtcctcg cggtccgagc
    10921 cggtggtggg cagcccgagc acgtcgaaga acaccacgta ggggatgggc caggagaggt
    10981 cctgcgcgat gtcggcctcg cccgcgtcgg cgaaccggtc gaccagcccc gtgaccaccc
    11041 ggcggacgtc gtcctccagc ttcgcgatcg cccgcggcag gaacgtccgc tgcacgatcc
    11101 ggcggagctg gtcgtggcgc gggttgtcga tgtcgggcag gaaacctggt gcactcatgt
    11161 ccttggccgt gtcgtcgatg tcgatgcact cgaacgacag gaaggtctcg tggcgcgcac
    11221 gtcgtcgagc cgggacaggg cgtagaagcc ccaccgctcg ctgacgtaga ccggcgcggt
    11281 gtcccggagg tgccggtaga ccggccacgg gtccgcctgg aaagcggcgg agtaggggtc
    11341 gtagacgacc ggggggctga gcgtcgtcac cgcgttcctc ctcgatgcgg agcgtggctt
    11401 taaagcgctt taaacacgag tactatgcga gacgcgccga cggcgtgtca agtgtgcgag
    11461 gggggtcgtc ctgagcgtcg tgaacccggg ccgcaggccc cggctggagg acgtcgccga
    11521 ggacgtcggc gtatccaccg catcggtgtc gctcgtgttg cgtggtgtgg ccggcccgag
    11581 cgcggagacc cgcaagcggg tgctggccgc ggcgacgcgg ttgggttacc ggcccgaccg
    11641 caccgcgagc ctgctggcgc gcaggcgcac gcacctgctc ggcgtgcctg tcgttctgcg
    11701 cgacgcgttc cgcgcggagc tggccgagga ggtgcaggtc gctgcggacg agcgcggcta
    11761 ctccgtggcg ctcggcgcgg tcaccccggt gcacgacgag gaccgcgtgg tggaaaccct
    11821 gctcgacctg cggtgcgagg cactcctgct gctctcaccc gagctccgcc ccggggcgct
    11881 cgcggagctc ggagggcggg tcccggtggt cgtagtgggc cggcacgtcg agccggccgg
    11941 tttcgacgtg gtgcgggtgg ccgacgacga gggggtcggt caggccgtcg accatctcgt
    12001 cgggctgggg caccgcgatc tcgtccacgt cgacggcgca ggcgcgccga tggccgccga
    12061 ccgccgctcg gggttcctgg ccgccacccg tcggcacggc cttgacagcg ctcgcgtgct
    12121 gcggggcggc tacgacgagg cggccggagc agcggcggct cgtgcactgc tcgacgagcc
    12181 cgtgctcccc accgcggtcc tcgcggctaa cgaccgcagc gcgatcggcc tcctcgacgt
    12241 cttcctccgc ggaggtgtgc gggtgcccgg cgatgtctcg gtggtcggct acgacgacag
    12301 cggcctcgcc gggctcgcgc acgtcgatct gacgaccgtg agccaggagg ccgctgccca
    12361 ggcggcccgg gcggtcgagg cagccgtgga gcgactggac ggggggcgga gcgagccgcg
    12421 ggaggtcgtg ctcccaccgc ggctggttgt gcgggggacg acggggccac cacgacaggc
    12481 cgggagaggc ggatgacccc cgcatcggcc gcgccgtcgc tcgtccggag aacctgagca
    12541 cccgattccc ggcggccgtg tgcgccgcgg ctgagctcca gggtagggac agccctgccg
    12601 gagagatcca ggacgacccg gtgggtttcg gcgccccgga actcaaggat catggacatg
    12661 gttctggttc agcagtcgag gagtgcccca ccgcgagatg tcgtggtgct ggccgaggtg
    12721 tcgaagtcct atccgggtcg agcggcggtg cgcgcgctgg accgcgtgac ggtgggcttc
    12781 gccagtggct cgttcaccgc cgtgatgggc ccgtccggat cggggaagtc gacgctgttg
    12841 cactgcgcgg ccggccttga ccggccgagc gaggggcgcg tgctgctcgc gggccaggac
    12901 atcagcgcgc tgcgcgaacc gaagctcacc gtcgtccggc gtgcccatgt ggggttcgtt
    12961 ttccagtcgt tcaacctggt cgacgcgctg tcggtgtggg acaacgtgct gctgccgcag
    13021 cggctcgccg ggaagcggcc cgatgaggcg tgggcgcggg aggtgctggc gcgggtcggc
    13081 cttgcgggac gggagaaggc gcggccgggc gagctgtccg gcggtcagca gcagcgtgtg
    13141 gcgctggcca gggcgctcgc cgggcgtccc gaggtgatct tcggtgacga accgaccggt
    13201 gccctcgatc tctccaccgg ccgcgaggtg ctggcgctgc tgcgacggtt cgtggacgag
    13261 ttcggagcga cggtggtgat ggtgacgcac gacccggccg cggcggcctg ggccgaccgc
    13321 gtggtgttcc tcgccgacgg ccggctcgcc ggagacctga tctcgccgac tgccgcgcag
    13381 gtgtcgaccc gtatggtgga gctgaccaga tgatcatcaa gctggccgcc gggaccgtgc
    13441 ggcgccgtcg gggcgccttc gccggaacct tcctcacggc gttcctggcc gtcgcgctga
    13501 tcgccggcag cggcctgctg ctgttctcgg tgctcaccgc agggcccggc gcggatcggt
    13561 tcgccgcagc cgacgcggtc gtcgccggtg accgggcggt ggtgctgacc accgagcagg
    13621 acaagggcga cggcgaggtc aaggtcaaga ccaagagcga gcggctgacc ggcgcgcccg
    13681 ccctgcccgc tgagctcgtc actgccgtcg cgggcacgcc cggtgtcgcc acggcggtgc
    13741 cggacgcggc gttcccggtc gcggtcgacc tcggcggggt cccggtgcgg gcgccggacc
    13801 gtgcggccgt ggtcgcgcac ggctgggcct cggctgcgct gaccccctac gaactgaccc
    13861 agggcgcacc gcccggtccg ggtggagtcg tgctggacgc cgcgctggcc gacgggctgc
    13921 ggatcggcga ggccgtccgg gtgaccaccc ggaccggcgc gcgggacctg cgcctggtgg
    13981 gcgtcgccgc accggccacg gccgagggtg tgccagggca ggccgcggtg ttcgtcgccg
    14041 acgcgcaggt cggcgagctg tccgggctgg ccgggccgac cgcggtcggt gtgcgggtgg
    14101 cgccgggcgc agacgccgcg gcggtggtcg cgggcctgca gcagcggatc gggggcgcag
    14161 ctgcggtcca caccggtgcc gacaaggtca cggccgatct gccgggtgcg ctgccggact
    14221 acatcggggc gatctcgatc ttcgggttcg tgctggggat caccgggttc gcggcggtgt
    14281 tcgtgctcac cgggacggtg tcgctcgcga tccgccagcg gttgcgggag ctcgccctgc
    14341 ttcgcaccgc gggggcgacc ccgcgccagt tgcgccgcat gttgagcctg gagatcgcga
    14401 tcgtgaccct cgtggccgct gcgccggccc taccggtcgg agtcgtggtg gcgcacctcg
    14461 tggcagcgcg gttccgcgat ctcggcgccg tcccgccgca gttcaccgtg gcgctgaacg
    14521 tcattgtgct gatcgccgcc gtcgtcgtcg ggctgctcgt gacgctgctc tccacggccg
    14581 tggcggccca ccgggcggtc cggatagcgc cgacacaggc actgcgcgag accgtgacgg
    14641 cttcgggcgg tggccgggtg ctgcgggtcg tcgtggcggc tgtgctggtc gccggcgcgg
    14701 tggcggtgct cggcctggtg ccgctgggcg gtgtgtacgg gatggggatg ggcttcgtgt
    14761 cctccgcgct gctgctgtgc gccgtcgccg ctctcgggcc gctcgcggtg ggcgcggtga
    14821 ccgcggtggt gtcgcgggtg gtcgcactga gcagtgtgct gggccggatc gccgcggtga
    14881 tgacccgggc ggagagtcgc cgggtcgtgg gagtggcgat cccgctgacg ctcatgttcg
    14941 cgatcaacgc cacgatgctg atcgacggcg agatcctcac cagggtgacc gccgaccaac
    15001 aggccgcccg taccgcctcg gcgaccgtcc gggtcgccgc cacgaacgcg ccaggtctcc
    15061 cgctcgacgt cgccgggcag gtgtgcgagc tgccggcggt gaccggttcg gccgccacca
    15121 tcggcacgcg cgtgatcgtc gatctcgggg gcaagccgga ggactacccg gcgcagggcc
    15181 tgcaggtctc gggcgagcag gctctcgacc tcggcgtcac ggcggggagc ctggacgatc
    15241 tgggccgcga cggtccggat caggccacgg tcgccgtgag cggcccgctc gccgaggagc
    15301 ggggctggaa gatcggcgac cggccggact tctggctggc cgacggcacg cggacgcccc
    15361 tgaccgtggt cgccgtgttc gagaactggc gtggcttcgg cgacctggtg ctgccggccg
    15421 agctcgccgc ccggcacgat ccccgtggcc tggtcggcgc cctctacctc cgcggcgagc
    15481 ccggggtggc cgaggcggtc cacgcggagt ggcccgcgct cgccgcgacg gacgcccgtg
    15541 acccccgggg cggcaccgcc gacgtgcaga accagcaggc cgcgtgggag ctgatggtcg
    15601 tcatctcgat cgggttcacc gcgatcgcgg tggtcaacac gttcgccatc gccacgagcg
    15661 cgcgccgtag cgagttcgcc gggctccggc tgatcggcgc gaccgcgccg cagctgcacc
    15721 ggctgctcgg cctggaggcg ctggtcaccg tggccgtcgc actggtgctg ggctgcctga
    15781 tcaccggcgg cgtggtgggc accttcagcg tggcccagga cgggcagtgg cggctgttcg
    15841 ccgacccgct gcgctacctg gccatgatcg gcggcgtcgg gttcctcggt ctggtggccg
    15901 cggcggtgcc ggcccggctg gtcgtccggc gccgatcgct ccccgggcag gcctagtttg
    15961 cgcttcctcg acactaggtt ctccaggtga tcgtccgtgc ggccagggaa ctggcctacc
    16021 tgctggccgg cgtgctgatc ggacccgcca cgttcctgtg ggccctgatc accacgctgg
    16081 ccagtggcct gttgtcggtg acctacctcg gaccgcccgt cttcctcgtc ctcacgtggg
    16141 tgacccggcg tctcgccgga ctggagaggt ggcgggcggg tgccctgctg ggcaggccgg
    16201 tgcccgcccc ctacgagccc gccagcggag cgaacccgtg ggcccgcggg cgcacgctca
    16261 cccgggagcc cgctacgtgg cgggacctca cctggctggt gttgatgttc ccgctcgggc
    16321 tcgcgaccgg gatacccggt gtcgtcgtcg cggtcgtcga tctcgggacg atcctcgcgc
    16381 ccctctggtt gtgggccgtg cccaacccgc acctgccccc ggtcgtcgac acgctgttca
    16441 acaccgtccc gggccgcttc gcgctcacgc tcctcggtgt cctgctggcc cttcccgtgc
    16501 ggtggctggt cggcaccctc gcgaggacgc aggccgaact cgcggtgaag ctgctggggc
    16561 agggcccccg gcagcgcctc gccgaccggg ccaccgagct cgccgtcacc cgtcgccggg
    16621 tggtggacgc gcaggccgcc gagctgcagc ggatcgagcg cgacctgcac gacggtgcac
    16681 aggcccgcat cgtggcagcg gggatgacgc tggcgctggc cgaacgcaag ctgcgtgcca
    16741 ccggtccgca gggtgacccg gcccgcgacg acctcctgac cgcccgccgg cagctcgacg
    16801 aggcgctcgc cgagctgcgc aggctggtcc gcgggatcta cccgccgatc ctcaccgacc
    16861 ggggcctcgt cgcggcgctg ggcgcgctgg cgggcgacgc atcgctgccg gtcgtggtgc
    16921 gcgccggcga cctcggtgaa ctggcgcccg cggtgcaggc ggccgcctac ttcgtggtcg
    16981 ccgaggcact ggcgaacacc gccaagcacg cgacggccga cgagtgcgtc gtggacgttg
    17041 tccgccgcga ggacgtgctc tcgctggagg tccgcgacga cggccgcggc ggggcggacc
    17101 cgtccggatc gggactcacc gggctgcggc gccgggtcga ggccctggac ggcaggatgg
    17161 aggtcacgag tccacccggc ggtcccaccg tcgtccgagc ggagctgccg tgcgcatcgt
    17221 gatcgccgag gatctgctcc tgctccggga cggcctgatc cggatcctca ccgacaccgg
    17281 gcacaccgtc gtcgcggcgg tggacaacgc ccccgacctc gagagcgcgg tgcgggagca
    17341 ccgccccgac ctgtccatcg tcgacgtccg gctgccgccg gggttccggg acgagggtct
    17401 gcgggccgcg ctgtcggtgc gggggcgcga gccgaggacg ccgatcctga tcctctccca
    17461 gtacgtcgag cgcacgtacg ccgccgagct gcttgccgac ggcaacggcg ccgtcggcta
    17521 cctgctcaag gaccgggtca ccgcgctgga cgagttcctc gatgcgatcg aacgggtcgc
    17581 cgggggcggc acggtgatgg acccggaggt cgtccggcag ctgttcgctc gcaacaaccg
    17641 cgaccagggc gtctcggcgc tcactccgcg cgagcgggag gtgctcgcgc tcgtcgcaca
    17701 ggggctgtcg aactccgcca tctgcaccga actggtgctc gccccaccct cggtggagaa
    17761 gcacatcacg aacatcctga ccaagttgga actgccaccg gccgacgaca cccatcgccg
    17821 ggtgcgggcc gtcctggcgt acctcaacca ctgagtggac gtcggctacc cgtcgtaggg
    17881 ggtggggtcg aggcgggttg cgtcgcggga gcgccggagc tcgcgcatct gctcggcgat
    17941 ggccacttcg accgcggccc ggacggcagc ggccgagacg tcagggtcga ccgacctggc
    18001 ggcggcaaga gaccgtgacg agacggagat gttgatcacg atcgtcagct ttcccgatgg
    18061 gtggggagcg ggtcccagca ggtccccgcg agcggtcatc aggcttatcg cctcggcgac
    18121 ggcgtttgat gccacccaca cgggtgatct cattcacgct ttgcggaccg ctacgagccc
    18181 agcaccgacc gtaggtgctc cacgctgacg cggatgtcgg ccagcgggcc ctccccgcgc
    18241 ggctcctcgg tgaggatcgt gtcctgctcc agcacgtacc acccgtcgta cccctgcccg
    18301 cgcaggtggc ccacgatcgc ctcgaagtcg acgtcgccct ggccgagcgg gcggtacatg
    18361 ccctcgcgga ccgcctcggt gtaggtgcgc ctgccagctt gcacctggtg ggccagcgtg
    18421 acgtccacgt ccttgaggtg ggtgtgggcg atccggtgcg gtgcttcccg ggtgagcgcc
    18481 gccgggtccg tgccgccgat gagcaggtgg ccggtgtcga ggcacagtga gatcgacgag
    18541 ccgtccagca cccgctgtac ctcctcgccg ttctcgacca acgtgccgat atgcgggtgc
    18601 agcaccgcgc ggacgccgtg ctcgatggcg agacccgtca gtcggtccag gttggtcagc
    18661 agcgtgcgcc agccggcgtc gtcgagggcg gggcgggcgt cgtacccggc caggccggtg
    18721 accgccgaca ggaccaacac gtccgacccc gtcgccgcgt aggcctgcag cagcttctcc
    18781 acctcgggga ccgggtcgcg gtcggccagg tgcagcagca gcggggtgaa cccgccgacg
    18841 gcctgcaggt ggtgccggga gagcacctcg gccatcgcgc ccggttcacc agggagaaaa
    18901 ccgtcgggcc ccagctcggt ggcggcgagc cctacctccc gcatctccgc gagcacccgc
    18961 gcgagcggca gctggtagcc ccagccgggt acctcacaga ctccccagga gatgggcgcc
    19021 ccggcgatcc tctggtcatt cactctgcag ttcctccagt tcgtacagca cgccgttggc
    19081 cgtgcgcagt ggcttcaccc cgacggggcg gcccgactcc cacgaccgcc gcgccgccct
    19141 ggccaggtcg aacgcggcga gcgcgtcgtg gccggttacc cgcggagcac cgccgtcgcg
    19201 cacgcagcgc ccgaaggcgt cgagctcgtc ggcgtaggcg tcggggtaac gctcctcgaa
    19261 gtcgcgcggc agctcgtggg acgcaagccc cggcgtgcgc cactcgtagt ggcgccgctg
    19321 tggggcgtcg atccgcacgg tcgcggcgct acccatcacc tcggtggagc actcgtagcc
    19381 gtaccgggcc gagcggctgt tgtcgatgac gccgagggct ccgttctcga aacgcagcac
    19441 gacgaccgcg gtgtcgatgt cgccgagcgc ggcgaacgcc gggtcggtcg ccgccccgtg
    19501 cgcgctcacc tccacgacct cgcctaccag ccagcgagcg acgtcgaggt cgtgcaccgt
    19561 gacgtcgacg aagaacccgc cggagccggt gaggtactcg ggaggcggag acgtcatgtc
    19621 ccgcaacgat gtgcggaaca gcgtgaccgc acccagctcg ccggcgcgga tccgctgggc
    19681 ggcggccacc cagtccgggt cggagcggcg gtggaacccc acctggagca ccaccccggc
    19741 ctgccccacc gcatcgatca cggcgagcgt ggtgtcgcgg tccagcgaaa tcggcttctc
    19801 gcagaagatg tgcttgcccg ccgctgcggc cgccatcgcc agctcggcgt gcgacccggt
    19861 ggacgtggcg atcgcgacgg ccttgacacc atcgtcagcc agtagctcgt cgaagctcgc
    19921 cgtccagggc acctcgagct cttcggcgag ctgccgggcg accgcggtgt ccgcgtcgta
    19981 cacgcacgcc agccgggccg aggcgcagcg cgaaaccaga ttcctcgcgt gaatgcgccc
    20041 catccggccg agcccggcga gcccgacggc aacgcggcgg ctcacgcgct gcccacggcg
    20101 tggcggccca ggtactcctg caccttctcg cggttgaaga tcagcgactc ccgcgcccgc
    20161 tcctcccagg cgaagacgca caccgtggcg atcccgtcga acccgacctc ggcgagcgtg
    20221 gcgaagaaca ggtcccagtt gacctccccc tggcccacgt ccaggtgctg gtggatgcgc
    20281 gctgtcgacc cgggcgggtt gacgatgtag cgcagccccg acgagccggt gtggtcgaag
    20341 gagtcggcga tgtgcagctg ggtgagcagt ggtcccgcgt accggatgac gccggcgagg
    20401 tcgccgccct ggtggaacgt gtgcggcgcg cagtacagga acgtcacgag ctcgttgtcg
    20461 atcccgcgga tcaggtcgac cgcgccccgc ccgtcctcca tgaagtcgtc ggggtgcggc
    20521 tcgagcacca gccgcacccc ctcccgctcg aacaccggga gcagctcctc catcgagcgc
    20581 cagaactgcg cctcgctggc cgcggcctgc tcggggcgcc cgttgaactc ggagttcatg
    20641 acgccacagc cgaggtcgac ggtgatctgg atggcgcggc gccagtaccg caccgcggcc
    20701 tgccgttcgt cctcgtcggg gcccgaccac ttgtacaacg gcagcaccga ggcgatctcc
    20761 acgccggcgc cggcgagctc cttgcggaac gccgtgattc tcgcccggtc cgcccgtggg
    20821 tgcacgaaga agggcaggaa gtcctcgcgc ggggacagct cgatgtgctg gtacccgaac
    20881 tccgcgacca gcgccgggag ctccagcagc ggggtcctgc gcagcatgta ggggtcgacg
    20941 gcgagcttca cctggcggcc tccttgtaca ggtcggggcg ttcgcgcagc accaccggga
    21001 cgcgcgtccc ggagtgcagt gcggcgagcc ccgtctcgca cgccacggtg gccgcgtagc
    21061 cgtcccacgc gctcgggccg gtggtgccgc ccgcggtgac ggcgtcgatc cacgcctgga
    21121 actcggtgtc gaaggcgcgg acgaaacgct cccgccagtc ggcgggcacc cggccggcga
    21181 tccgtccggc gctcttgacc agggcgcccg cgctctccga cagctcgacg gtgccgtcct
    21241 cgcagaccac ctcgccgcgg atgtcgtagc cgaagcccgc gttcaccatg gcctcgacgt
    21301 ccacgagtac tccactgcgc atctccagca gcacgagcag cgggtcgttg aaacccgggg
    21361 cggccttgcg gctcacccgc ggcgtgaaca cggtgatggc gtcgatctcg tcgtcgaaca
    21421 tccaccgggc gacatcgatg tcgtgcaccg tgctgtcgtt gatgatcatg tcgctggtga
    21481 agtgcgacgg caccgacggg ttgcggtgcg ccgcgtgcat gagcagcggc agcccgatct
    21541 cgcggccggt caccacgtcc ttcatagccc ggtactgcgc gtcgtagcga cgcatgaagc
    21601 cgaccatcac cagccgccgc cccgccgcga cctcggcgtc gacgatccgg gcgcaggcct
    21661 cctcggtggt cgcgagcggc ttctcgcaga agacctgctt gcccgccgcg atgcatgcca
    21721 ggacgtactc ctcatgggtc ggcccccagg acgtgacgac caccgcgtcc acgtccggat
    21781 cggcgatgag gtcctggccg ctggtgtgca gcacggcggc ggggaggtcg tccgcgaccg
    21841 tccgggcgcg gtcgaggtcc acgtcggtga ccgcggcgac ccgcgccccg gacaggacgt
    21901 gggtgagcct cctgatgtgg tcctgcccga tcatgcccac gccgatgacg ccgacgccta
    21961 gagtcacgtc gtttcctctc ctggtctgtg tgcgtgcgct agcgcttgag ctcgtgggac
    22021 agctcgtcga gctcctgccc gcccgccatc tccgcggtga gctcgtcgag ggtcaccccg
    22081 gcgcgcttct tgtcgaggac gcagcgaccg agcttcagga tgatgaagtg gtcgcccacc
    22141 atgtaggcgt ggtgcgggtt gtgggtgatg aacacgacgc cgagccccgc gtcccgcgcc
    22201 gcggccgtgt acttgagcac cacgccggac tgcttgacgc ccagcgcggc ggtcggctcg
    22261 tcgaggatga ggacgcgggc gccgaagtac acggccctgg cgatcgcgac acactggcgc
    22321 tggccgcccg acagtgtccc gatcggctgg ttgatgtcct tgacctcgat gcccatcttc
    22381 cgcagctcgc tgtcggcgat ccgccgcatg ccggtgatgt cgagcggggc gagggggtag
    22441 gtgcccttgc gcagctccga gccgaggaag aagttgcgcc acacctccat caacgagacc
    22501 accgcgaggt cctggtagac cgtggcgatg ccgtggtcga gcgactcgcg cggcgaggcg
    22561 aagtgcacct gctccccgtc gaccttgacc acgccctcgg tgtgcgggtg caggccagag
    22621 atgatcttga tgagggtcga cttcccggca ccgttgtcgc cgaggacgca ggtgacctcg
    22681 ccggcgttga ccgtcaggtt gatctcctcg agcgcccgga tggcgccgta ggccttgccc
    22741 acgttctcca tctggaccaa cggcgttccg gcctggagat cctgtgcggc gtgttccgca
    22801 atcgtgtcgg tcatgccggt ctcaccgcct ctggctcgcg tagttcttga cgtacaggtt
    22861 gacgagcacg gccagcagga gcatcgcgcc caggaaggcc ttgaaccagt tcgggtccca
    22921 gccggcgaag acgatgccct gggtgaccat gccgaagatg aacgccccga gtgcagagcc
    22981 caccgccgag ccgtagccgc cggtgagcaa cgcgccaccc acgaccgcgg cgatgatgta
    23041 gatgaactcg ttgcccacgc ccaggccggc ctgcacgctg ctgaagttga acagcaggtg
    23101 ctggccgagg aaccaggcca ggaacgcgac gcccatgaac aggccgatct tgaccttggg
    23161 cacgggcaca ccgaccgcac gcgcgctggc ggcgttgcca ccggaggcga agatccagtt
    23221 gccggtgcgg gtacgcagca gcacccacgt ggcgacagcc acgaacagga tccaccacag
    23281 caccgtgatc tcgagcgtca tgaagccgag atcgatgttg ccggcgaaga acgcgcgcgc
    23341 cgaggtgaac ccgtcgagcc ggtcgatgtt ggtggtggcc acagtgccgg tgacgagctt
    23401 ggtgacgccc aggttgacgc cctggagcac gaagaacgtg ctcagggtga tgaggaagct
    23461 gggaatcccg gtccgcacca ccaggtagcc gttcacgaag ccgatagcca gcgcgatgac
    23521 caggctgatg accacgccga cccacgcgtt caggctgaac tggtaggaga tcatcgccga
    23581 ggcgagggct gcggtggcca cggcgacacc ggccgagagg tcgaactccc caccgatcat
    23641 gagcagggcc acgccgacgg cgacgatgcc gatggtcgag ctggcgtaca gcaccgtcga
    23701 gaacgccgga aggctccgga agggcgccgc gaccgcgaag aagaagatga agatggcgat
    23761 cgcggccgcg agcgcgccga tctccggacg ccccagcacg cgggacacca gcgaccgccg
    23821 gacgcgtgcc ggttgttggg cggatgggga ctgcggcggc ggcgatgtcg tcacggtcga
    23881 gctcatgact ggctccaggg gtgggcggcg cgggggagcg gggcatgccg gctcccccgc
    23941 ggtggaacta gcgagtgttg ttcgcggcgt acgtcgcgat cttgtcgatg ttgctcttgt
    24001 cgacgaacga cggtccggtc agcacgggct tgccgccgcc gatgtcgttg ccgttgctga
    24061 ggttcagcca gagcgcctgc acggccatgt agccctggac gtagggctgc tggtcgatgg
    24121 agaagtcgat ggttccgtcc tggatgtcct tggcgacgtc ggcgttcagg tcgaacgtga
    24181 cgaccttggc ctggctgctc gtgccctgct tggcctgcag cgcggcgagc gcgatcggtg
    24241 cgccgagcgt gacgacgtcg gtgatcgagg ggtcctgctg gagcttcgcg ccgatcgtct
    24301 gttgcaccga cggcagatcc gtgccgttga cctggaggtt ctcggtgttg gggaaggtct
    24361 tcttcacacc ggcgcagcgg gcttcgagcg cgacgctgcc ctgctcctgc accacgcaga
    24421 tcgccttgcg gcctccggcg gcggccattc tctggcccgc cgactgcccg gcgaggtcct
    24481 cgtcggaccc gaagtacatg gggatgccgt acttcgtgta ctcgttgatg cccgagttga
    24541 aggcgacggt cgggatcccg gcctggaccg ccttctgcgc ggccggtccg acctgatctg
    24601 cgtacgccag cgtgacagcg atcccgtcga ccttgctgtc gatggcgttc tgcaccagtg
    24661 tcgcctgctg gccggcgtcg ttgctgttgg agtacttcag gtcgacgttg agcgtcttcg
    24721 ccgcctgctg ggcacccgcg cgcaccttgt cccagaatga gtcgcccggc gcctcgtgcg
    24781 tgaccatcgc gatcgtgtac tgcttgccgc cggcgctgcc gacggcgggc ccactgttct
    24841 ggttggcctg gtttgcaccg ccctggctgc tgcacgcggc caacgcgacg ccgagcgctg
    24901 cggcgactgc cagcgctcgc atcgacttcc cagtactcct cattgagttt ccttccagtg
    24961 aacagccggc cgcggtggcg ggctggggtt gcgtcagtag ggccaccgtc ggaccgggcc
    25021 gagcccacag ccgcggaagt agccggccgt gcgggcgccg atcgggagcg ggatctgcgg
    25081 ctcgaccggg tagaggtcct gctcgaccac ggtgtacaga tcggtgtcca gcgccgccaa
    25141 tgcgtcgagc agcggtggca tttcgggaac gccgtacggc ggctccacca tcacgcccag
    25201 cgggactgcc tcggcgaggg acagccgctg ctcgcggacc cgcctgcgga tctcggggtc
    25261 cacctgcttg aggtgcacgt acctgatccg ctccgggaag cgccggatga tggcgagatt
    25321 gtcgccgtcg cagtaggaga tgtgaccggt gtcgagacac aggtcgacgt actcggggtc
    25381 ggtgtcctcg aggaaccgct ccacccgttc ctgggtgtcg acgtgggtgt c
//
LOCUS       DASRSC010000162         4572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1529155,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000162 DASRSC010000000
VERSION     DASRSC010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4572
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529155"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFE39_07890"
     CDS             <1..792
                     /locus_tag="VFE39_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258556.1"
                     /translation="HVLAAGSSYRTLRQRSRSPGALIEKESALVHLADSLDGWPGGGS
                     GGYRATASSGWCTTSYRLMTRAWFTVQTGETKGSTMGNRPDETQSIEYEAKVLNVDPD
                     NIDRRVLSQGGVKVADRLMRRYVYDIEPGDMSRWIRLRDTGTSVTLATKRIRHDGIDG
                     TDEVEVGVDSFEKTHALLGAMGFVAKAYQENRRVSYRLNDCDVELDSWPLIPVYVEIE
                     GRSRDHVIEVARLLGFTETDLTGENTTKIYSRYGIDLSSISDLRL"
     gene            798..1085
                     /locus_tag="VFE39_07895"
     CDS             798..1085
                     /locus_tag="VFE39_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET6258557.1"
                     /translation="MAVYGYARARVQPALLAQEIQLRAAGCDTVVSDMASSRELRDGW
                     QRLVEAAITGDTIRVVGWSSLTGNPTEYRLTVRTLAARGVRVEALGGPPPF"
     gene            complement(1220..1498)
                     /locus_tag="VFE39_07900"
     CDS             complement(1220..1498)
                     /locus_tag="VFE39_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HET6258558.1"
                     /translation="MDEETVERVRAIVLAIPPGETSSYGEVAARAGLRSPRLVGRILA
                     MDGHDLPWHRVLRADGTCAPHIAVEQGARLRAEGVLLVDGRLPRPHRR"
     gene            complement(1503..2276)
                     /locus_tag="VFE39_07905"
     CDS             complement(1503..2276)
                     /locus_tag="VFE39_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6258559.1"
                     /translation="MSAAPLNVRTFGPRDGVPVLALHGVTGHSARWRVLAEALPEIRL
                     IAVDLRGHGRSPWTPPWHIEQHVADALAVLDDLGLARVAVAGHSFGGAIAVHLAHTAS
                     ERVERLMLLDPAIGLDAQDMLETAEASRADESFPDVPAARADRAQRWEGIGAELVDAE
                     LTDHLVPDGDRYRYRYCGAAAVVAWSEMARAALTPPAGTPTLLLPAAKADFVAPGWVD
                     ACRAELGDALTVTEIDAGHMVYLERTAEVADEMRRFMRT"
     gene            2427..>4572
                     /locus_tag="VFE39_07910"
     CDS             2427..>4572
                     /locus_tag="VFE39_07910"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HET6258560.1"
                     /translation="MWDEAAQRVLAHDSGPLRVVGGPGTGKTTLLLASVAGRIRAGAD
                     PERTLLLVGSRRAAGELRERLGEMVHGDGVVERTSREPLVRTVHSYAFGVLRLHAARN
                     GDPPPRLLASAEQDAVVRDLLAGELDGPEHGGAPDSGWPARLRPALGLPGFAAELREL
                     LLRAGERGLGPEELTELGRRHDLPEWYAAGRFFRSYEQVILLRGAAGRGAPQATAPAL
                     DAAELVSAALDALASDPELLAAERERVRHLVVDDAQDLDPQQMELVRALGSTAGTVLL
                     AGDRDQAVLNFRGADPNGLRAVDAETVVLTTDHRSAPAVRAAGARLVARLSGSVAGRE
                     RVAPPGDPDGPEGTVAVRVFGSPAQEAGWVADQLRRAHLSDGVPWSRMAVLARSTRRS
                     LPTLRRALLAAGVPVAAPPDELPLARQHAVVPLLMVLRCATRPDELDADAATALLTSP
                     LGSADPMRMRRLRRGLLRLHAGGRPARVAPEPDSQAEGDHAARPADIERSGSDPLLVE
                     ALQAAALGRPDPLVALPPQETAPLRRVGALLAIAGDAARDGESAEEVLWRVWQACGLG
                     PRWSEASARGGPVGAAADRDLDAVLALFDAAARHTDRLPGAAVASFVEYLADQQLPVD
                     SLAPRAPRGEAVVLLTAHAARGREWDVVAVPGVQEGSWPDLRLRGSLLGNERLVDLVA
                     GVAEPGRETVSRVAPLLAEERRLFYVACTRARH"
BASE COUNT          593 a         1596 c         1762 g          621 t
ORIGIN      
        1 catgttcttg ctgccggcag cagttatcga accctacgac agcgctcccg tagccctggc
       61 gcactcattg aaaaggaatc ggctctggtt catctggccg atagcctgga cggctggcca
      121 gggggcggct ccggcgggta ccgcgccacg gcgagttccg gatggtgcac aactagctac
      181 cgtctgatga cacgcgcgtg gtttacggtc caaacaggag aaacgaaagg ctcaacgatg
      241 gggaatcgcc ccgacgaaac gcagtccatc gagtacgaag ccaaagttct gaacgtcgat
      301 ccggacaata tcgatcgccg cgttctatcc caaggagggg tgaaggtcgc cgaccggctg
      361 atgcgccgct acgtctacga catcgagccc ggcgatatgt cgcggtggat caggctgcgc
      421 gacaccggca cttcggtgac gttggcgacc aagcgcattc gccacgacgg aattgatggc
      481 accgacgaag ttgaagttgg ggtcgacagt ttcgagaaga ctcacgccct actgggggcg
      541 atgggcttcg tcgcgaaggc ataccaggag aaccggcgcg tcagctaccg cctgaacgac
      601 tgcgatgtgg aactcgactc ctggccgctt atccccgtct acgtcgagat cgagggccgg
      661 tcgcgcgatc acgtgatcga ggtcgcccgc ctgctcggat tcaccgaaac cgatctcacc
      721 ggcgagaata cgaccaagat ttacagccgg tacgggatcg atctgtccag catcagcgac
      781 ctgcggttat aggggccatg gccgtctacg ggtacgcgcg ggcgagagtg cagcccgccc
      841 tgctggctca ggagattcag ctccgcgcgg cggggtgcga cacggtggtc tcggacatgg
      901 cgtcgagcag ggagctgcgc gacggctggc agcggcttgt ggaggccgcg atcaccggtg
      961 acacgatccg agtggtgggg tggtcgtcgc tgaccggcaa cccgacggag taccgcctga
     1021 ccgtgcgcac gctcgccgcg cgaggagtgc gggtggaggc gctgggcggg ccgcctccgt
     1081 tctgatctgg catcacggac ggcccgctcc cacgtcgggg gcgggccgtt ccttcaggcg
     1141 ggtgctctcc cgacatgtgt ccatgtcgcg tccggggccg gagcgggcag gtgggggcgt
     1201 gcggccggcg ggagcggtgt cagcggcggt gggggcgggg gaggcggccg tcgaccagga
     1261 ggacgccttc ggcccgcagg cgggcaccct gctcgacggc gatgtgcggg gcgcacgtcc
     1321 cgtccgcccg cagcacgcgg tgccacggca ggtcgtgccc gtccatcgcg aggatgcggc
     1381 caacgagccg tggcgagcgc agcccggcgc gggcggcgac ctcgccgtag ctcgacgtct
     1441 cgcccggcgg gatcgcgagc acgatggccc gcacccgctc gaccgtctcc tcgtccacgg
     1501 gctcaggtcc tcatgaaccg gcgcatctcg tccgcgacct ccgcggtgcg ctccaggtag
     1561 accatgtgcc cggcgtcgat ctcggtgacg gtcagcgcgt cgcccagctc cgcacggcag
     1621 gcgtccaccc atcccggggc gacgaagtcg gccttcgcgg caggcagcag gagggtcggc
     1681 gtgccggcag gcggggtgag cgccgcccgc gccatctcgc tccacgctac gacggcggcc
     1741 gcgccgcagt agcggtagcg gtagcggtcg ccgtcgggca cgaggtggtc ggtgagctcg
     1801 gcgtccacga gctcggcccc gatgccctcc caccgctgcg cgcggtcggc gcgagcggcg
     1861 gggacatcgg ggaaggactc gtcggcgcgg ctcgcctcgg ccgtctccag catgtcctgc
     1921 gcgtcgaggc cgatcgccgg gtcgagcagc atcaaccgct cgacgcgctc ggacgcggtg
     1981 tgcgccagat gcacggcgat cgcgccgccg aaggagtgcc cggcgacggc gacgcgcgcg
     2041 agaccgaggt cgtcgagcac ggccagcgcg tcggccacgt gctgctcgat gtgccatggt
     2101 ggcgtccacg gcgatcgccc gtggccgcgc aggtcgacag cgatcagccg gatctcgggc
     2161 agcgcctcgg ccagcacccg ccagcgcgcc gagtgaccgg tgacaccgtg cagggcgagg
     2221 acgggcacac cgtcgcgtgg cccgaaggtg cggacgttga gcggtgcggc agacacgtcc
     2281 gtaatcgtgc cagcagcact ccccgccgtc gcggtcccgg ggggtcgtgg ttgcatcgct
     2341 gcgtgtcccg tccctaccgt ccgcctgccc gtgccgcgct cgcgccgggg ctcgtccgca
     2401 cggcgcgggc gacgccgggc gagctggtgt gggacgaggc cgcgcagcgg gtgctcgccc
     2461 acgacagcgg gccgctgcgg gtggtcggcg ggccgggcac gggcaagacc acactgctcc
     2521 tggcgagcgt ggccgggcgg atccgggcgg gggccgaccc cgagcgcacc cttttgctgg
     2581 tcggaagcag gcgtgcggcg ggggagctgc gcgagcggct cggcgagatg gtgcacggcg
     2641 acggcgtggt ggagcgcacg tcgcgcgagc cgctggtgcg cacggtgcac tcctacgcgt
     2701 tcggggtgct gcggttgcac gcggcacgca acggcgaccc accgccccgg ttgctcgcga
     2761 gcgccgagca ggacgccgtc gtgcgcgacc tgctggccgg cgagctcgac ggaccggagc
     2821 acgggggagc gcccgactcg ggctggccgg cccggctgcg ccccgcactc gggctgccgg
     2881 ggttcgccgc cgagctgcgg gagctgctgc tgcgggcagg cgagcgtggc ctcgggcccg
     2941 aggagctcac cgagctcggc aggcgccacg acctgcccga gtggtacgcg gcgggccggt
     3001 tcttccgcag ctacgagcag gtcatcctgc tgcgtggggc agcagggcgc ggcgcgccgc
     3061 aggccaccgc gccggctctg gacgccgccg aactcgtgtc ggctgcgctg gacgcgctgg
     3121 cgtccgaccc ggaactgctc gcggccgagc gggagcgggt gcggcatcta gtcgtcgacg
     3181 acgcccagga cctcgacccg cagcagatgg agctcgtgcg tgcgctgggt tccacggcgg
     3241 gcacggtgct gctcgccggc gaccgggacc aggccgtcct caacttccgt ggtgccgacc
     3301 cgaacggcct gcgtgccgtc gacgccgaga cggtcgtgct caccaccgac caccgcagcg
     3361 cacccgccgt gcgggccgcg ggggctcggc tggtggcgcg gctgtccggt tccgttgccg
     3421 gacgcgagcg ggtggcgcct cccggcgacc cggacgggcc ggagggcacg gtggcggtgc
     3481 gggtgttcgg ctccccggcc caggaggcgg gctgggtcgc cgaccagctc cgtcgtgccc
     3541 atctgtccga cggcgtcccg tggtcgcgca tggcagtgct ggcccgctcc acccgccgtt
     3601 ccctgcccac gctgcgccgg gcgctgctgg ccgcgggcgt gccggtggcc gcgcctcccg
     3661 acgagcttcc actcgcccgg cagcacgctg tcgtcccgct gctgatggtg ctgcgctgcg
     3721 ccacccgtcc cgacgagctg gacgccgacg ccgcgaccgc cctgctgacc tctccactgg
     3781 gctccgcgga cccgatgcgg atgcgacggc tgcgtcgcgg gctgctgcgg ctgcacgccg
     3841 ggggacggcc tgcgcgcgtg gcacctgagc ccgactcgca ggccgagggc gaccacgccg
     3901 cgcgcccggc cgacatcgag cgctccggga gcgacccgct gctggtcgag gcgctgcagg
     3961 cggcggcact cggccgtcca gatccgctcg tcgcgctgcc accgcaggag acggctccgc
     4021 tgcgccgggt cggcgcgctg ctggcgatcg cgggcgatgc tgcgcgcgac ggggagagcg
     4081 ccgaggaggt gctctggcgg gtgtggcagg cctgcgggct ggggccgagg tggagcgagg
     4141 cgagtgcacg cggtggcccg gtcggcgcgg cggcggaccg cgatctcgac gccgtgctcg
     4201 ccctgttcga cgcagctgcc cggcacaccg accggttgcc cggtgccgcc gtggcgagct
     4261 tcgtcgagta cctcgccgac cagcagctcc cggtcgactc gctggccccg cgggcgccga
     4321 ggggcgaggc ggtggtgctc ctcacggcgc acgccgcacg ggggcgcgag tgggacgtcg
     4381 tggccgtgcc gggggtgcag gagggcagct ggccggacct gcggctgcgc ggcagcctgc
     4441 tcggcaacga gcggctcgtg gacctcgtgg ccggcgtggc cgagccgggc cgggagacgg
     4501 tgtcgcgagt ggccccgctg ctcgccgagg aacggcggct gttctacgtc gcgtgtacaa
     4561 gggcccggca ca
//
LOCUS       DASRSC010000163        13344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5784272,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000163 DASRSC010000000
VERSION     DASRSC010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 13344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13344
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5784272"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1134)
                     /locus_tag="VFE39_07915"
     CDS             complement(208..1134)
                     /locus_tag="VFE39_07915"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET6258561.1"
                     /translation="MADKAFRIMRAGAGSRPVMLAIAGDSASGKTTLTRGLVQALGPD
                     NCVSICVDDYHRFDRAERRDLPFTALHPDCNHLDIMEQHLQLLASGQPILKPVYDHST
                     GELARPERVEPNQYIIVEGLFPLYSKLARACFDVTVYLDPPEEIRRRWKLTRDTRARG
                     YSRPQVLDELNRRIAESEAYIRPQRSHADIVVRFAPIAVRDDPPGTQLSAELVLRPTI
                     RHPDLSGVLANHRTMHVKLCRDTDGRPVDTLHVHGYVPTEDSRTLEKAMWALLDQPGD
                     VPDGLGVLGWGQRSEPLAITQLLLLFHMLDAG"
     gene            1309..1965
                     /locus_tag="VFE39_07920"
     CDS             1309..1965
                     /locus_tag="VFE39_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6258562.1"
                     /translation="MPGPLRIVLVDDHEMVRHGLKAMLAPFRTRVRVVGEAEGADDAV
                     PLVDGLDPDIVLSDVRLNGESGLDLCRRLCEERAGRRVVLLSVYDDEQYLFQALRAGA
                     AGYLIKRITGEDLVRHLELVHAGETVIDPSMAGRAVASAARLHSGEFWPGARHGLTHR
                     ESEVLSLIVTGLSNRAIATRLVVGDETVKSHLRSVYRKLAVNDRAGAVAAALREGIFQ
                     "
     gene            1962..3182
                     /locus_tag="VFE39_07925"
     CDS             1962..3182
                     /locus_tag="VFE39_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HET6258563.1"
                     /translation="MTASRRPALGGLADLEAENALLLAIVEATSSGPDVEPLAAAVAR
                     LIVEATATDVCFVHVLDDAGRSLTLAGATPPFDCEVGTVRLPIGTGVAGWVAEHGEPA
                     VIVDDKHADARYLPIPALRGTDYTSMASVPMTSDLAGLVGVLNVHTVVRREFTPRDIR
                     LLTSIGSLVAGALHSARLHRRLVSRERANERFTEQVIAAQETERRRLAGDIHDGISQR
                     LVSLGYHLDAVAGTMRSDPAYAEQQLALARELADITVDEARIAIGGLRPPVLDDLGLA
                     GGLASLCRTVPDLDYELTLGDERLPEHVEIALYRIAQEALQNVQKHARASTVQLRFAV
                     WAGTARLEIGDDGCGFATGVGGLEARDLQPDGSGYGMRSMTERAELVGGTLTVRSRRG
                     SGTTVTATVPVPTG"
     gene            complement(3225..4166)
                     /locus_tag="VFE39_07930"
     CDS             complement(3225..4166)
                     /locus_tag="VFE39_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HET6258564.1"
                     /translation="MTTNARLRALVELADTGSVRGAAERLVVTESSVSAAVSALAADV
                     GVPLVRRNGRGVLLTPAGERYAGYARRILGLHDEAMAAARGEADPERGLLRLAAVTTA
                     GEHVVPALLASFRVRHPNVTLRLEVAHRARVWPLLAHHDVDLVLAGRPPDELAAGIRA
                     VSPNTLLVVGPPGLAAGFAPAAATWLMREAGSGTRDTCEALLAALEVDPPRLSLGSQG
                     AVIAAAEAGLGVTLVSRQAVARQLADGGLVELPMTGAPLYRPWHAVSHPHATAGTDLF
                     VTHLLARPELGWTAPPPLADAATGRTASAARDRHACP"
     gene            complement(4163..5230)
                     /gene="cbbX"
                     /locus_tag="VFE39_07935"
     CDS             complement(4163..5230)
                     /gene="cbbX"
                     /locus_tag="VFE39_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HET6258565.1"
                     /translation="MCAVRMNGLGDGSPRRGFAMPGSAVAPTPEVAPVDDGAAPSLAP
                     DAVVDLAAERSATGVDGVLDALDAELVGLAPVKARIAEIAALLLVDRTRARFGITTAP
                     PNLHMCFTGGPGTGKTTVALRMAGLLHRLGYLRRGHLVTVTRDDLVGQYVGHTAPKTR
                     DVLKRAMGGVLFIDEAYYLHKAENERDYGSESIEILLQVMENHRDDVVVILAGYAAEM
                     DVFFRSNPGMSSRIAHHITFPAYTVDELEQIADRMADQLGYVFAPDTRPVLRDYLERR
                     MARPRFANARSVRNAVERARLRQARRLVDGADPRVDRDALTTIEPADLLASRVFDPEP
                     DGTEPGERGTAEGCGSGTMPE"
     gene            complement(5331..5756)
                     /locus_tag="VFE39_07940"
     CDS             complement(5331..5756)
                     /locus_tag="VFE39_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HET6258566.1"
                     /translation="MRITQGTFSHLPDLTDDEITAQIAYCLDNGWPLSVEFTDDPHPR
                     NTYWEMWGLPMFDLADPAGVMLEVDTCRTSHPDCYVKLNAYDARLGRQTTALSFIVQR
                     PEPEPGFRLDRHEGADRRITYTTTSYATGRPQGERYPAT"
     gene            complement(5766..7196)
                     /locus_tag="VFE39_07945"
     CDS             complement(5766..7196)
                     /locus_tag="VFE39_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="form I ribulose bisphosphate carboxylase large
                     subunit"
                     /protein_id="HET6258567.1"
                     /translation="MSDRWSAGVIPYAEMGYWQPDYVPKDTDILCAFRITPQDGVPPE
                     EAGAAVAGESSTATWTVVWTDRLTAHEHYQAKCYKVEAVPGASGQYMAWIAYDLDLFE
                     EGSIANLTSSIIGNVFGFKALKALRLEDMRIPTHYVKTFAGPPHGIVMEREYLGKYGR
                     PLLGATTKPKLGLSARNYGRVVYEALRGGLDFTKDDENINSQPFMRWRDRHLFCMEAV
                     NRAQAATGEIKGHYLNVTAGTMEQMYERAEFARELGSVIVMIDLTVGYTAIQSMSAWA
                     RRHGVLLHLHRAGHGTYTRQKTHGVSFRVIAKWMRLAGVDHIHAGTVVGKLEGDPTIV
                     AGYYDTLRLDHVPADPVKGLYFDQDWASLPGVMPVASGGIHAGQMHQLLHHLGEDVVL
                     QFGGGTIGHPMGIAAGATANRVAVEAMIKARNEGRDVMVEGAEVLKTAAGRCRELDVA
                     LATWGDITFDYAATDTPDVVATPTGV"
     gene            7488..7727
                     /gene="ndhC"
                     /locus_tag="VFE39_07950"
     CDS             7488..7727
                     /gene="ndhC"
                     /locus_tag="VFE39_07950"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HET6258568.1"
                     /translation="MDEYLRSYALVGLLLMLGAGFVAVSYGANQLLRPHRPTREKVLG
                     YECGLDPVGEGWAQSHVRYYVFESLGDMLCPEPGR"
     gene            7724..8329
                     /locus_tag="VFE39_07955"
     CDS             7724..8329
                     /locus_tag="VFE39_07955"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HET6258569.1"
                     /translation="MTGVDAEEIALLAGAALAMGEPEIGHGLLTVTVAPTAWCHALEW
                     ARDELDCSLFDFLCGVDELDADPPGILVVAHLHCPAGRHHLLLRTLVPADDLRLPTAT
                     GVYRGANWHERETHEMFGVDFTGHPNLDPLLLPDGFEGLPLRKDFVLASRVVKQWPGA
                     VNPGQSAGDAARRPGKRPPGVPDPARWGPAAQPGPGTDPGA"
     gene            complement(8518..10215)
                     /locus_tag="VFE39_07960"
     CDS             complement(8518..10215)
                     /locus_tag="VFE39_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6258570.1"
                     /translation="MLIVTINMSILLIALPDIFRGIGIDPLAPANISYLLWLIMGYLV
                     VTAVLVVSFGRLGDIFGRTRMYNLGFAVFTVFSVLLAVTWTHGDAAAIWLIVMRVLQG
                     VGGALLLANSTAILTDAFPADQRGLALGINQVAAIAGSFLGLVVGGLLAPLNWHWIFL
                     VSVPVGVFGTIWAHYKLRETGVRGPARLDWWGNVTFAVGLVAVLVGITYGIQPYGTHV
                     MGWTSPTVLGLIIGGLVVLVGFAVIETRVPDPMFRMSLFRIRAFTAGNLANLLAALGR
                     GGLQFILIIWLQGIWLPEHGYAFEQTPLWAGIYMVPLTIGFLVAGPVSGLLSDRLGAR
                     PFSTAGMLLAAVSFLLLDLLPVDFDYRLFAALLALNGLAMGLFSSPNRAAIMNSLPPE
                     RRGAGAGMTSTFQNGATVLSIGVFFSLLIIGLSGTLPRALFSGLVEQGVPPDVATRVA
                     GLPPVGTLFAALLGDNPIQNLLGPQVLGALTPAQAEFLTGRAFFPELISGPFAAGLTL
                     ALGFAAAACLVAAVASALRGGKFLYQEPQSGTGPQPSSTSWGSTESSAATSAASPMA"
     gene            11415..12344
                     /locus_tag="VFE39_07965"
     CDS             11415..12344
                     /locus_tag="VFE39_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET6258571.1"
                     /translation="MDALSEVLPMLRIRSASPSRLEASGEWNATFRGCQHIKVAAVVS
                     GSCWVTADDGVPLRMDAGDCYMLSAGIPYRIASNLEAPLLDGNDLFTCATSSTVLLGD
                     RPDDANRTVLIGGMVIFDETTAALLLDSLPPVTRIGASTHPAHVLHPTLQLLADETAV
                     ALPGRTAMSEHLTEILFLQVLRSHLADHEGDDRQSLGWLGALNDPQIGAALALMHREP
                     GRRWTVAALATSAAMSRSAFALRFKTLVGLSPLDYLLQWRIRAAEQALKVTHRSVASI
                     AAEWGYESESAFSNAFKRVTGHRPTHYRGTVGS"
     gene            complement(12369..>13344)
                     /locus_tag="VFE39_07970"
     CDS             complement(12369..>13344)
                     /locus_tag="VFE39_07970"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HET6258572.1"
                     /translation="LIVRGHHFRLPVRTTLVVVAVAVLLGGAAIMVEGRARAGDGIPA
                     AVAQPSVVAPVAAPALAAGEAAGETAGETAASAAAVKAAVQAVDALDRASPNTKFGAA
                     VLDRTTGQLAVGAGGATAFYSASVVKLYTVVAILHRVETGSVTLTAADRTNIQRALVA
                     SDDQAMNALWERFGGPQTVAQTISLVGLKDSKPPDDSDSGEWGETLISARDVVAVYDY
                     VLRSMAPSSRDMIMGALEQAQDTGADGFDQAFGLIAPPRKPDVAAKQGWMWIGSDFWL
                     HSTGVLGADDRYVIAVLSKNPGQPSSAAARTIVTTAVSDAEAALPGPW"
BASE COUNT         1964 a         4735 c         4711 g         1934 t
ORIGIN      
        1 ctggctcgcc ggctaccagg tgacccaccc cgagcgcgca gtcgacgagc ggctgctggc
       61 gctggcctgg gtccgctacc gccagacgct cagcctgggc atcgaccgcg ccgaccattc
      121 ccacagcggc tgggccgccc ccgtccgcct cgggtgagtc gcaccgcctg gggtgctggt
      181 catttcagcg ggacctgggt gggtgggtca gccggcgtcc agcatgtgga agagcagcag
      241 gagctgggtg atcgccagcg gctcgctgcg ctgcccccag ccgagcacgc ccagtccgtc
      301 cggcacgtca ccgggctggt cgagcagcgc ccacatcgcc ttctcgaggg tgcggctgtc
      361 ctcggtgggg acgtagccgt gcacgtgcag cgtgtcgacc ggcctgccgt cggtgtcgcg
      421 gcacagcttc acgtgcatcg tccggtggtt cgccagcaca ccgctgaggt cgggatggcg
      481 gatggtcggc cggagcacga gctcggccga gagctgagtg cccggcgggt cgtcccgcac
      541 agcgatgggc gcgaaccgga cgacgatgtc ggcgtggctg cgctgcgggc ggatgtaggc
      601 ctccgactcg gcgatccgcc ggttgagctc gtcgagcacc tgcggccggg agtagccccg
      661 cgccctggtg tcccgggtca gcttccagcg gcggcggatc tcctcgggcg ggtcgaggta
      721 caccgtgacg tcgaagcagg cgcgggcgag cttgctgtac agcgggaaca ggccctcgac
      781 gatgatgtac tggttgggct cgacgcgctc cggccgggcc agttctcccg tggagtggtc
      841 gtagaccggc ttgaggatgg gctggccact ggccagcagc tgcaggtgct gctccatgat
      901 gtcgaggtgg ttgcagtcgg gatgcagcgc ggtgaagggc aggtcgcgcc gctcagctcg
      961 gtcgaaccgg tgatagtcgt cgacgcagat cgagacacag ttgtcggggc ccagcgcctg
     1021 caccagcccg cgggtgagag tggtcttgcc ggacgcgctg tcccccgcga tcgcgagcat
     1081 gaccgggcgc gagccagccc cggcgcgcat gatccgaaat gccttgtccg ccatcctcca
     1141 cgcaccgcct ctccgccgcg cccgacaccg ccatcagtgc cccggccgca gtggcatcga
     1201 ccgtaggcgg atcacggcgc catcgagtcc cccgcaacgg gggtcctacg ggggaggcgc
     1261 tcgcacagag ggtgtccgac cggctgtggc agcaactatc gtcagcagat gcccgggccg
     1321 ctgcgtatcg tcctcgtcga cgaccacgag atggtgcgcc acggcctgaa ggcgatgctg
     1381 gcgccgttcc gcacccgggt ccgggtggtc ggcgaggcgg agggcgccga cgacgcggtc
     1441 ccgctggtcg acggcttgga ccccgacatc gtgctctccg acgtccggct caacggggag
     1501 agcggcctcg acctgtgccg gcggctctgc gaggagcgag ccgggcggcg ggtggtgctg
     1561 ctgtcggtgt acgacgacga gcagtacctg ttccaggcgc tgcgggcggg ggcggcgggc
     1621 tacctgatca agcgcatcac cggggaggac ctggtgcgcc accttgagct ggtacacgcc
     1681 ggggagaccg tcatcgaccc cagcatggcc ggtcgcgcgg tcgcctcggc ggcccggctg
     1741 cacagcgggg agttctggcc gggcgccagg cacgggctca cccaccggga gagcgaggtg
     1801 ctctccctga tcgtcaccgg tctgtccaac cgcgcgatcg ccaccaggct ggtggtcgga
     1861 gacgagacgg tgaagagcca cctgcggtcc gtctaccgca agctcgcggt caacgaccgc
     1921 gccggtgccg tggcggccgc cctgcgcgag ggcatcttcc aatgaccgcc tcgcggcgcc
     1981 ccgcgctggg cggcctcgca gacctcgagg cggagaacgc gctgctgctg gccatcgtgg
     2041 aggccacctc gtccggaccg gacgtcgagc cgcttgccgc cgcggtcgcc cggttgatcg
     2101 tcgaggccac tgccaccgac gtctgcttcg tgcatgtgct cgacgacgcc gggcgctcgc
     2161 tcacgctcgc cggcgccacc ccgccgttcg actgcgaggt cggcacggtg cggctgccga
     2221 tcggcacggg ggtggcgggg tgggtcgccg agcacgggga gcccgccgtg atcgtcgacg
     2281 acaagcacgc ggacgcccga tacctgccga tcccggcgct gcgcggcacc gactacacct
     2341 cgatggcctc agtgccgatg accagtgacc tggccggcct ggtcggcgtg ctcaacgtgc
     2401 acacggtggt ccgccgcgag ttcaccccgc gcgacatccg gttgctcacc tcgatcggca
     2461 gcctggtggc gggcgctctg cactcggcgc gcctgcaccg caggctcgtc tcccgcgaac
     2521 gtgccaacga gcggttcacc gagcaggtga tcgccgccca ggagacggag cggcggcgac
     2581 tggcaggcga tatccacgac gggatctcgc aacgtctggt cagcctcggg taccacctgg
     2641 acgcagtggc cggcacgatg cgcagcgacc cggcgtacgc ggagcaacaa ctggcgctgg
     2701 cccgtgagct ggccgacatc acggtggacg aggcgcggat cgcgatcggc gggctgcggc
     2761 cccccgtgct ggacgacctc ggcctcgccg gcggcctggc cagcctatgc cggacggtgc
     2821 ccgacctcga ctacgagctg accctcggcg acgaacggct gcccgagcac gtcgagatcg
     2881 cgctgtaccg gatcgcgcag gaggcgctgc agaacgtgca gaagcacgcg cgggccagca
     2941 ccgtccagct gcggttcgcg gtgtgggcgg gcacggcgcg cctggagatc ggcgacgacg
     3001 gctgcggctt cgccaccggg gtgggcgggc tcgaggcgcg cgacctgcag ccggacggct
     3061 cggggtacgg gatgcgcagc atgacggagc gggcggagct ggtgggaggc acgctgacgg
     3121 tgcgctcccg ccgcgggtca gggacaacgg tcacggcaac ggtccccgtc ccgaccggct
     3181 gaggcagtct gacggcgcga ggacggtgtg ctccccgccg cggctcaggg acaagcgtga
     3241 cggtcccgag cggctgaggc cgtcctgcca gtggcagcgt cagccagggg cggtggggcg
     3301 gtccagccca gttcggggcg cgccagcagg tgtgtcacga acaggtcggt gcccgctgtc
     3361 gcgtgcgggt gactgaccgc gtgccatggc cggtacaggg gggcgcccgt catcggcagc
     3421 tccaccaggc cgccgtcggc gagctgccgg gccacggcct gccgggacac gagcgtcacc
     3481 ccgagccccg cctcggcggc cgcgatcacc gcgccttgcg agccgaggct gagccgcggc
     3541 gggtcgacct ccagggccgc gagcagggcc tcacaggtgt cgcgggtgcc cgagcccgcc
     3601 tcacgcatca gccacgtcgc ggcggcgggc gcgaagcccg cggccaggcc gggcggaccg
     3661 accaccagca gggtgttggg gctgacggcg cggatcccgg cggccagttc gtcgggggga
     3721 cgtccggcga gcacgaggtc gacgtcgtgg tgggcgagca agggccacac cctggcccgg
     3781 tgagcgacct ccagtcgcag cgtcacgttc gggtgccgca cgcggaacga ggcgagcaac
     3841 gccgggacga cgtgctcccc cgccgtcgtc accgcggcca gccgcagcag cccccgctcc
     3901 gggtcggcct cgccgcgcgc cgccgccatc gcctcgtcgt gcagacccag gatgcgccgg
     3961 gcgtacccgg cgtagcgttc cccggctggg gtgagcagca ccccgcggcc gttgcggcgc
     4021 acgagcggca ccccgacgtc cgcggccagc gcgctgaccg cggccgagac cgacgactcg
     4081 gtgaccacca ggcgctccgc ggcgccccgc accgagccgg tgtcggccag ctcgacgagg
     4141 gcccgcagcc gcgcgttcgt ggtcattccg gcatggtccc cgacccacac ccttcggcgg
     4201 taccccgctc gcccggctcc gtgccgtccg gttcggggtc gaacacccgg ctcgccagca
     4261 ggtcggccgg ctcgatcgtg gtcagcgcgt cccggtcgac ccgcgggtcc gccccatcga
     4321 ccagccggcg cgcctgacgc agccgggccc gttccacggc gttgcggacg ctgcgtgcgt
     4381 tggcgaagcg ggggcgggcc atgcggcgtt ccaggtagtc gcgcagcacg ggccgggtgt
     4441 cgggggcgaa gacgtacccc agctggtcgg ccatccggtc ggcgatctgc tccagctcgt
     4501 cgaccgtgta ggcgggaaac gtgatgtggt gggcgatccg cgagctcatg ccggggttgc
     4561 tgcggaagaa cacatccatc tcggccgcgt acccggcgag gatgacgacc acgtcgtcgc
     4621 ggtggttctc catcacctgc aggaggatct cgatggactc gctcccgtag tcccgctcgt
     4681 tctccgcctt gtgcaggtag taggcctcgt cgatgaacag cacgccgccc atcgcgcgct
     4741 tgaggacgtc ccgggtcttg ggcgcggtgt gtcccacgta ctgcccgacc aggtcgtcgc
     4801 gcgtgacggt gacgaggtgc ccgcggcgca ggtagcccag ccggtgcagc aggcccgcca
     4861 tccgcagcgc caccgtcgtc ttgccggtgc ccgggccgcc ggtgaagcac atgtgcaggt
     4921 tcggcggggc ggtcgtgata ccgaaacggg cccgggtgcg gtccaccagc agcagcgcgg
     4981 cgatctcggc gatccgcgcc ttgaccggcg ccagccccac cagctcggcg tcgagagcgt
     5041 ccaagacccc gtcgacgccg gtggcgctgc gctccgcggc gaggtcgacc accgcgtcgg
     5101 gcgccagcga aggcgcggcc ccgtcgtcca ccggcgccac ctccggcgtg ggcgccaccg
     5161 cgctacccgg catcgcgaac ccgcgtcgcg ggctcccgtc cccgagaccg ttcatccgga
     5221 cggcacacat ctcgtcgcca ccccgcatac gtcaggacgt gtgtgcggtg gccgcgacgt
     5281 gtgcgcggtc gcgccggctc tcacgtcgcc gcctctcaca tcccggcgtc tcacgtcgcc
     5341 gggtatcgct cgccctgcgg gcggcccgtc gcgtaggacg tcgtcgtgta ggtgatccgc
     5401 cggtccgcgc cctcgtggcg gtccaggcgg aagccgggct cgggctcggg gcgctgcacg
     5461 atgaacgaga gcgccgtcgt ctgcctgccg agcctggcgt cgtacgcgtt gagcttcacg
     5521 tagcagtcag ggtgagaggt gcggcacgtg tcgacctcga gcatcacccc ggccgggtcg
     5581 gccaggtcga acatcggcag cccccacatc tcccagtagg tgttgcgcgg gtgcgggtcg
     5641 tcggtgaact ccaccgacag cggccagccg ttgtcgaggc agtacgcgat ctgcgcggtg
     5701 atctcgtcgt cggtcaggtc gggaaggtgg gagaaggtcc cctgggtgat tcgcacgaag
     5761 gctcctcaga ccccggtggg cgtggcgacc acgtccgggg tgtcggtggc ggcgtagtcg
     5821 aacgtgatgt cgccccaggt ggccagcgcg acgtcgagct cgcggcagcg cccggccgcc
     5881 gtcttcagga cctccgcgcc ctcgaccatg acgtcccgcc cctcgttccg ggccttgatc
     5941 atcgcctcga cggcgacgcg gttggcggtg gcccccgcgg cgatgcccat ggggtggccg
     6001 atcgtgcccc cgccgaactg caggacgacg tcctcgccga ggtggtgcag cagctggtgc
     6061 atctggccgg cgtggatccc gccggacgcc accggcatca ccccgggaag ggacgcccag
     6121 tcctggtcga agtacaggcc cttgaccggg tcggcgggca cgtggtcgag ccgcagcgtg
     6181 tcgtagtacc cggcgacgat ggtcggatcg ccctccagct tgccgaccac ggtgccggcg
     6241 tggatgtggt cgacgccggc gagccgcatc cacttggcga tcacccggaa gctcacgccg
     6301 tgggtcttct gccgcgtgta ggtaccgtgc ccggcccggt gcaggtgcag cagcaccccg
     6361 tggcgccgag cccacgcgct catcgactgg atcgcggtgt agccgaccgt cagatcgatc
     6421 atcacgatga cgctgccgag ctcgcgggcg aactccgcgc gctcgtacat ctgctccatg
     6481 gtgccggcgg tgacgttgag gtagtggccc ttgatctccc cggttgcggc ctgcgcgcgg
     6541 ttgaccgcct ccatgcagaa caggtggcga tcgcgccagc gcatgaacgg ctgggagttg
     6601 atgttctcgt cgtccttggt gaagtccagc ccgccgcgca gggcctcgta gacgacgcgg
     6661 ccgtagttgc gcgcggagag cccgagcttg ggcttggtgg tggccccgag cagcgggcgg
     6721 ccgtacttgc ccaggtactc ccgctccatc acgatcccgt gcggcggccc ggcgaacgtc
     6781 ttcacgtagt gcgtcgggat ccgcatgtcc tccaggcgca gggccttgag cgccttgaag
     6841 ccgaacacgt tgccgatgat cgagctcgtc aggttcgcga tcgagccctc ctcgaacagg
     6901 tcgaggtcgt aggcgatcca tgccatgtac tgcccggacg cgcccggcac ggcctcgacc
     6961 ttgtagcact tggcctggta gtgctcgtgc gcggtgagcc ggtcggtcca gaccacggtc
     7021 caggtggcgg tgctcgactc ccccgccacc gctgcgccgg cctcctcggg cggcaccccg
     7081 tcctgcggcg tgatccggaa ggcacacagg atgtcggtgt ccttcggcac gtagtccggc
     7141 tgccagtagc ccatttccgc gtaggggatg acgcccgcgc tccaccgatc cgacatgggg
     7201 cgagcatgaa cccccaaacc tgaccgatca atcaagtctt cacagctgat acttcgccat
     7261 acactcaagt attggtcggg gtatcgccac ggtcggcccg ggacggcacg ctgcgcccgt
     7321 gacgtgggtg gggtggcggg aggccaccga gcaggcgctc tgcagcgggt gaccggactc
     7381 ttgggtgaat ggttgacggt tcactgtcga ctctccatag acggttcggg tgtcgggcct
     7441 catcgtggaa cagcgccagt tccccggccc ccagcgacgg gatgcccatg gacgagtacc
     7501 tgaggtcgta cgcgctggtc gggctgctgc tcatgctcgg agccggcttc gtggcggtga
     7561 gctacggcgc caaccagctg ctgcgcccgc accggccgac ccgggagaag gtgctcggct
     7621 acgagtgcgg tctcgacccg gtcggcgagg ggtgggcgca gtcgcacgtc cgctactacg
     7681 tcttcgaatc gctcggtgac atgctgtgcc cggaaccggg ccggtgacgg gcgtggacgc
     7741 cgaggagatc gccctgctgg ccggggccgc gctcgcgatg ggcgaaccgg agatcggcca
     7801 cggcctgctg acggtcaccg tcgcgcccac cgcgtggtgc cacgctctgg aatgggcgcg
     7861 cgacgagctc gactgctcgt tgttcgactt cctgtgcggc gtcgacgagc tcgacgccga
     7921 tccccccgga attcttgtgg tggcgcacct gcattgcccg gcgggacgcc accacctgct
     7981 cctgcgcacg cttgtgcccg cggacgacct ccggttgccc accgcgaccg gcgtataccg
     8041 cggcgcgaac tggcacgagc gggagaccca cgagatgttc ggggtggact tcaccggtca
     8101 cccgaatctc gacccgctgc tgctcccgga cggcttcgag ggcctcccgc tgcggaagga
     8161 cttcgtgctc gcctctcgtg tcgtgaagca gtggccgggc gcggtcaacc ccggccagtc
     8221 cgcaggcgac gccgcccgcc gccccggcaa gcgcccgccg ggcgtccccg accccgcacg
     8281 ctggggaccg gcggcgcagc cgggacccgg caccgaccca ggggcctgac ccggtgatgc
     8341 tgctgctcga catcggggtc cggctgggct gtggtcaccg gcggctacgg gtggctggcg
     8401 gtactggtcg tggtcaacac cgcgatcggg ctcgcctact acctcgcctg ggcggtccgg
     8461 ctcctcgcgc ggcgggaacg gggcgccccg cgttcggttc gctacggccc tcggtcgtca
     8521 cgccatcggg ctcgccgcac tcgtggccgc gctgctctct gtgctgcccc agctggtgct
     8581 cgacggctgg ggtccggttc cactctgagg ctcctggtag aggaacttcc cgccgcgcag
     8641 cgccgacgcg accgcggcaa ccaagcacgc cgccgcggcg aacccgaggg ccagcgtgag
     8701 gccggccgcg aacggcccgg agatcagctc cgggaagaac gcgcgtccgg tgaggaactc
     8761 ggcctgcgca ggcgtcagcg ccccgaggac ctgcggtccg agcaggttct ggatcgggtt
     8821 gtcgccgagc agcgccgcga agagcgtgcc gaccggcggc aggcccgcca cgcgggtcgc
     8881 gacgtcgggg ggcacgccct gctcgacgag tccggagaac agcgcacgtg gcagcgtgcc
     8941 ggacagaccg atgatgagca gcgagaagaa caccccgatc gacaggaccg tggccccgtt
     9001 ctggaacgtc gaggtcatgc cggcaccggc gccgcggcgt tcgggtggca ggctgttcat
     9061 aatcgcggct cggttgggcg agctgaacaa ccccatcgcc agcccgttca gcgcgagcag
     9121 cgcagcgaac agccggtagt cgaagtcgac gggcaacagg tccagcagga ggaagctgac
     9181 ggcggcgagc agcatcccgg ccgtcgagaa cgggcgcgcg ccgagccggt ccgagagcaa
     9241 cccggacact gggcccgcca cgagaaagcc gatggtcagc ggcaccatgt agatccctgc
     9301 ccacagcggc gtctgctcga atgcgtagcc gtgctcaggc aaccagatcc cctgcagcca
     9361 gatgatgagg atgaactgca gcccaccgcg gccgagcgcg gcgagcagat tggccaggtt
     9421 gcccgcggtg aaggcgcgga tccggaacag cgacatgcgg aacatcgggt ccggcacccg
     9481 cgtctcgatc accgcgaagc cgaccaggac gacgaggccg ccgatgatca acccgagcac
     9541 ggtcgggctg gtccagccca tgacgtgcgt tccgtagggc tggatgccgt aggtgatgcc
     9601 gacgaggacc gccacgagcc cgaccgcgaa cgtcacgttg ccccaccagt ccagccgggc
     9661 gggcccgcgg acgccggtct cgcgcagctt gtagtgcgcc cagatcgtgc cgaacacacc
     9721 gaccggcacg gacaccagga agatccagtg ccagttcagc ggcgcgagca gacccccgac
     9781 cacgagcccg aggaacgacc cggcgatcgc cgccacctgg ttgataccca gggcgaggcc
     9841 gcgctggtcg gcagggaagg cgtcggtgag gatcgccgtc gagttggcca gcagcagggc
     9901 gccaccgacg ccctgcagca cgcgcatgac gatcagccag atcgcggcgg cgtcaccgtg
     9961 ggtccacgtg accgccagga gcacggagaa gaccgtgaag accgcgaagc cgaggttgta
    10021 catccgggtg cgcccgaaga tgtcaccgag ccggccgaag ctgaccacca gcaccgcggt
    10081 gaccacgagg taccccatga tcagccagag caggtagctg atgttggccg gggcgagcgg
    10141 gtcgatgccg atgccacgga agatgtcggg cagtgcgatg agcaggatcg acatgttgat
    10201 cgtgacgatc agcacgccga tcgtggtgtt ggagagggca acccactagt agtggccgcc
    10261 cgtcgagtta tttagcatta ctaacaatat acgtccaagc gaggtccgtc cgcatccccc
    10321 tggacgatcc gcccgccgtc agccggagcg tctgacacag cgggcgatcg gcggggtcca
    10381 cgtcccccac gatcagagct tccaccaccc cggccaccgc ggccgtcgcc gtccgctgca
    10441 gagaccttcg ccggtcgcct gactgctcga tgagggcagg ctcgaggacc cacagtagga
    10501 gctccgcact ccgtcgacgg ccttgtgccg tacgtccgcg ggatcgaccc ggctcagcag
    10561 caccagcagg ggcgccagct cagcgagctc ggcacgacag ggggcgcaac cggcgagatg
    10621 gcgatcgaac tcagcactct cggacgaaca gaggctgccg agcagatagg agcccagcgc
    10681 gaggcagatg ccctcgtgct tgctcaccat gcctccctat cggagaagtc agcagcagcc
    10741 ctcatcacat gccccctgac gggcagacac ggcatccggt tcaccgcgag cccggcgtgt
    10801 ggcccacacc atcacaggcg tcctcgatcg acgccgtccg gggcccgttc acggttcggg
    10861 ggcctcgtca ggctcgcacc acgaacgcgg ggcagcacat gacgtccggc gcaaaacgat
    10921 cagaaggggt ccccattcac ctgccggtcg gcccgatcga accgcaccag gagaaactcg
    10981 cgcgttccgc gaccacagaa tccgacactc acctaccccc tgggagaaca ccgtctcgtt
    11041 caggaccagc cgcgctccgg tcgttcgccc tgcgacccac cgggggcacg ccgaccgggc
    11101 cgagggcttc ctccaggaga ccctgtcacc atcctggact gcggcgatgg ctcagatcac
    11161 acgttcggtg tcggagatat ctgaacccgg accggatacc ggctcgtaga ggtggacatg
    11221 gggatggtgg gccgacgcca agagcgcaca ggactccggc gggtcgcgtc cactggttca
    11281 ccccgcgctg gaaatgaccg ccgctgaggg tccccgtagg tgggtccgga cggccattcg
    11341 atccggcgcc gttccacggc ataccaggtc gcgcctctgc gctcggcggc tgattctcgc
    11401 atgatggagt tgttgtggat gcactttccg aggtcttgcc catgctgcgc atccgcagcg
    11461 cgtcgccgtc tcgtctggag gcgagcggag agtggaacgc gacgttccgg ggctgccagc
    11521 acatcaaggt cgccgccgtc gtgtccggct cctgctgggt gaccgccgac gacggtgtac
    11581 ccctgcgcat ggacgccggt gactgctaca tgctgtcagc agggatcccg taccggatcg
    11641 cgagcaacct cgaggcgcca ctgctcgacg gcaacgacct cttcacctgc gccacgtcca
    11701 gcacggtgct cctcggcgat cggccagacg atgcaaatcg gacggtgctg atcggcggca
    11761 tggtcatttt cgacgagaca accgcggccc tgctgctgga cagcctgccc ccggtcaccc
    11821 ggatcggcgc gagcacccac ccggcgcacg tcctgcatcc gaccctccag ctgctcgccg
    11881 acgagaccgc cgtcgctctg ccggggcgca ccgcgatgtc cgagcacctg accgagatcc
    11941 tcttcctcca ggtgctacgg agccacctcg ccgatcacga gggtgacgac cggcagtccc
    12001 tgggatggct cggcgctctg aacgatcccc agatcggcgc ggcgctggcc ctgatgcacc
    12061 gggagccggg caggcgctgg accgtggccg cgctcgccac gtccgccgcc atgtcgaggt
    12121 cggccttcgc cctgcggttc aagacgctcg tggggctctc cccgctggac tacctcttgc
    12181 agtggcggat ccgcgccgcc gagcaggccc tcaaggtcac ccaccggtcg gtggcctcca
    12241 tcgccgcgga gtggggctac gagtcggaga gtgcgttcag caacgccttc aagcgcgtca
    12301 ccgggcaccg gcccacccac taccgaggca cggtgggctc atagcccgtg ccctgccccg
    12361 agggcgggtc accatggccc gggcagcgca gcctcggcat cagacacggc ggtggtgacg
    12421 atcgtcctgg ccgcggcgga actcggctgc ccggggttct tgctgagcac cgcaatgacg
    12481 tagcggtcgt ccgcaccaag tactccggtg ctgtgcagcc agaagtccga gccgatccac
    12541 atccacccct gcttcgccgc gacatcgggc ttccgcggtg gcgcaatcag gccgaaagcc
    12601 tggtcgaagc cgtcggcccc cgtgtcctgt gcctgctcga gggcacccat gatcatgtcc
    12661 cggctgctcg gcgccatcga ccgcagaacg tagtcgtaca ccgcgacgac atcgcgcgcg
    12721 gagatcaacg tctctcccca ctccccgctg tcgctgtcgt ccggcggctt gctgtccttg
    12781 agcccgacga ggctgatggt ctgtgcaacg gtctgcgggc cgccgaacct ctcccacagc
    12841 gcgttcatcg cctggtcgtc gctggccacg agtgcccgtt ggatgttggt gcggtctgcc
    12901 gccgtgagcg tcacagaccc ggtctcgacg cggtgcagaa tcgcgaccac cgtgtagagc
    12961 ttgaccaccg acgcggagta gaacgccgtc gcaccaccgg caccgaccgc cagctgcccc
    13021 gtggtccggt cgaggaccgc ggcaccgaac ttggtgttgg ggctggcccg gtcgagggcg
    13081 tcaaccgcct gtaccgcggc tttcactgcg gctgctgacg ccgctgtctc cccggcggtc
    13141 tccccggcgg cctcccccgc agcgagagcg ggcgctgcca ccggcgcgac gacggacggc
    13201 tgcgcaaccg ccgccgggat cccgtcacct gcgcgggcac ggccttcgac catgatcgcg
    13261 gctccaccca ggagcacggc gacggcgacc accaccaggg tggttctgac gggcagccga
    13321 aaatgatgtc ccctcacgat cagg
//
LOCUS       DASRSC010000164        16742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5130664,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000164 DASRSC010000000
VERSION     DASRSC010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 16742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16742
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5130664"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1432
                     /locus_tag="VFE39_07975"
     CDS             <1..1432
                     /locus_tag="VFE39_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6258573.1"
                     /translation="VAAPRLSPDGRLLAWLQWNHPSMPWDDTELVVRDLGTGEVTVVA
                     GGPGESVAEPRWQPDGSLWFISDRTDWWNLYRWVPDADIEPVVRTDAEIGVPGWTFGS
                     ARFAVLADGRVVFARRRQGFDALAVRELDGSLVDLDLPFSAISAVRSTGPGTVAVVAG
                     SPAAESGVFLVDVATSAVEPLRPPRDLRVDPSMVSVPEAISFPSVDAAGERRTAHALF
                     YPPTHEQFRGPDGELPPLLVEIHGGPTSAAVPALSLPVQYWTSRGFAVVDVNYGGSTG
                     YGRAYREQLLGEWGVVDVADCMAAAEWLAAQGRVDRNRMCIRGGSAGGYTTLAALARA
                     DTPFAAGANYYGVADLAALAADTHKFESRYLDRLVGPYPEAREVYEERSPIHHVDRFD
                     RPLIVLQGAEDEIVPPSQSELIVDALRARRRPVAYLLFDGEQHGFRRAENIRRALDAE
                     LSFYAQVLDFELPADEGIEPVTVENL"
     gene            complement(1440..2318)
                     /locus_tag="VFE39_07980"
     CDS             complement(1440..2318)
                     /locus_tag="VFE39_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET6258574.1"
                     /translation="MSTSVLYDAPGPVTRRRTLISSGVASVIVLVVIALVIIRLASQG
                     QFSAERWGPMVNPFDPSFLAVWRLLGQALVATLVAAGLTLVFSLVIGTLFAVSRITAN
                     RYYRWLVVGVIEFLRGVPVVIAIFFAARVLPEVGLDLPILWYLVIGLTAYNSVIIAEI
                     IRAGVASLPRGQAEAGYSLGLTRGQVLSSILLPQAFRVMLPALISQLVVIVKDTSLGF
                     IISYEEFVRTANIIIQTLHNPIQTYAIVALIFIVINYGLGKLAEYVERRLSRAKRTAA
                     APADPATLDTAATGGG"
     gene            complement(2315..2965)
                     /locus_tag="VFE39_07985"
     CDS             complement(2315..2965)
                     /locus_tag="VFE39_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET6258575.1"
                     /translation="MGVIIEQFPLFVLGFLGTVRLCVFSAVGALVLGTILAGFRVSPV
                     PPLRMIGTAWVTVFRNTPLAVVLFAVAFGLPELGINGSYFYFGVIGLTCYTAAFVCEA
                     VRSGINSVSAGQAEAARAIGLTFSQSLSLIVLPQALRTVVPPLGSVIIAMIKNSAIAG
                     AFGIGGDLFANGVQLTSAQGYAAVGALTGALLGYLLLTIPGGILLGVLERKVAVAR"
     gene            complement(3035..3931)
                     /locus_tag="VFE39_07990"
     CDS             complement(3035..3931)
                     /locus_tag="VFE39_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6258576.1"
                     /translation="MRFSHLIRIAAVAATVVIAATACGDQSSSLGSSGAAPSVAQGAS
                     FPAGSTMDTLNKAQTLRLGTKFDQPLFGLKGLDGNPAGFDVEIAKIIAAKLGIPADKI
                     AYTEAPSAVREEVIEQGRVDMVAATYTINDKRKERISFAGPYYVAGQDLMVKSDNTTI
                     TGPDSLRGANAKVCSVAGSTPSENIKKYIDPANLTLFDVYSKCADALRTGQVDVVTTD
                     NVILTGLIENSGGAFKLVNQKFTEEPYGIGIKKGDVAFCEFINQTLKEAEADGSYKAA
                     WDSTVGKVSPEVPPLPAPGTCS"
     gene            complement(3996..4724)
                     /locus_tag="VFE39_07995"
     CDS             complement(3996..4724)
                     /locus_tag="VFE39_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET6258577.1"
                     /translation="MIRMASVDKHFGELHVLRDINLEVESGQVVVVLGPSGSGKSTLC
                     RTINRLEPIDSGTIEVDGKRLPEEGKALAGLRADVGMVFQSFNLFAHKTILENVTLGP
                     LKVRKVGKAVAGKDGLALLERVGIANQKDKYPAQLSGGQQQRAAIARALAMKPKVMLF
                     DEPTSALDPEMVQEVLDVMTALAGEGMTMLVVTHEMGFARRAAHRVVFMSDGEIVEDA
                     SPETFFSNPTTDRAKDFLGKILTH"
     gene            4853..5578
                     /locus_tag="VFE39_08000"
     CDS             4853..5578
                     /locus_tag="VFE39_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6258578.1"
                     /translation="MHILLVEDDDRIAAALRPALHRNGMTTTRLSRGRGAVDHLAGVD
                     VVLLDLGLPDIDGVDVCRAIREVSEVPVIVVSARGEVDDRILGLHSGADDYLVKPYDI
                     GELVARVHAVYRRRRVVSSAGPEDVVVVDNVSVDLHRHSVTVGGRPVNLTRKEFQVLA
                     LLASSGGAVCTRSRIVAEVWGRSWAGANRTLDVHVATLRTKLGRADLVQTVRGVGYRL
                     GLPESGNPDSVIPNSGVPDNGTE"
     gene            5581..7026
                     /locus_tag="VFE39_08005"
     CDS             5581..7026
                     /locus_tag="VFE39_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6258579.1"
                     /translation="MRTRLLVIVLFLVGLLAVGLGVPLALSNAQANQLELFTDRLTDT
                     IFYASLAERPITEADTTGLEQELARYDAVYGVAVMVVDRNGQLVATSRHPPPVLDEAG
                     RERVELALANRRSEAYPPLLPWDERPLVLAEPVLVDTEVRGATITVSPTAALRAEEVR
                     VWSLVAAAGLLALAMAVLVALPIVRWILRPVRRLDEGTGRVAAAVLAGAGAEPVADGS
                     GPPELRRLSVSFDRMAETVTQAYAAQRAFVADASHQLRNPLTALRLRLSNLEGHVDGS
                     AIEDHMAALEEADRLSTLLDGLLALARAERTAPVVVVEVDPAVADRIDAWRPLAEHTG
                     LQLVRCGRRGLRAAASAGAIETVLDAVLDNAVKFSPPGGTVTVRTGTTPTHVEVAVRD
                     TGPGLAPEELERATDRFWRSPAQSNVDGSGLGLAIAARTVELAGGELRLELPAGGGLQ
                     VIALLPQVPGEEPDGEQPVVEREAGMPSSPA"
     gene            complement(7115..8083)
                     /locus_tag="VFE39_08010"
     CDS             complement(7115..8083)
                     /locus_tag="VFE39_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HET6258580.1"
                     /translation="MEGGGSCGRRAVLGGLGLLGLLAALGGCSSPYASTRLTLATGGT
                     QGVYFGLGNALADAWQRKLGLDVRPEVLATAGSVDNLRLLATGAVDVAFSQVDTAADQ
                     LLKSAPGDQRAPRALARIYDDAVHVVVRATSPVTTLAGLRGARVSIGAPDSGVYVIAT
                     RLLGLAGVSPDTDLQAEQLGINESVAAMESGAIDAFFWSGGVPTDGVSRLARTVPIRL
                     LNLEDMITAVHTAYPAYASGTVPAATYGIPEPITTLLVRNFLLVPASMPDDLASALVE
                     GLFDAQPELAVASPSALTIDPRAAIGTQPVPLHPGAERFFRALKNG"
     gene            8148..9629
                     /gene="miaB"
                     /locus_tag="VFE39_08015"
     CDS             8148..9629
                     /gene="miaB"
                     /locus_tag="VFE39_08015"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HET6258581.1"
                     /translation="MGRSYTIRTYGCQMNVHDSERMAGLLEAAGYTKADSEDADVVVF
                     NTCAVRENADNRLYGNLGHLRPVKTARPGMQIAVGGCLAQKDRSEIVRRAPWVDVVFG
                     THNVHALPVLLERARHNATAQVEIAESLEVFPSTLPARRESAYAGWVSISVGCNNTCT
                     FCIVPSLRGTEKDRRPGDVLAEVSALAADGVLEVTLLGQNVNSYGVEFGDRGAFAKLL
                     RACGDVEGLERVRFTSPHPRDFTSDVIAAMAETPNVCPQLHMPLQSGSDDVLRRMRRS
                     YRSARFLAIIDEVRAAIPHAAISTDIIVGFPGETDADFEATLDVVRSARFANAFTFQY
                     SLRPGTPAAALPDQLPKAVVQERYERLVALQEEISWSANRALEGQVVEVLVSTGEGRK
                     DSATRRMSGRARDGRLVHFAPGPAASDAGGIRPGDIVEVAVGYGAPHHLVADGPVLSH
                     RRTGAGDVHEMGTRPGGVPTGLGLPSFGAPVQAAATPGCATSA"
     gene            9668..10144
                     /locus_tag="VFE39_08020"
     CDS             9668..10144
                     /locus_tag="VFE39_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258582.1"
                     /translation="MNERKVPEELAALDAELTGIGRRVQRQIDPGPAALGVSIAILVL
                     IVALILPWTGSVRGWEVLAGLEPRLGVLPRLFTFTSLGFGLVVSTLALATRWWALAWL
                     SAVGCGISVVTGLWAIWSRQVAVPVGGTGPGVGVVLALLAVITLAVCWARIAGRRG"
     gene            complement(10283..11545)
                     /locus_tag="VFE39_08025"
     CDS             complement(10283..11545)
                     /locus_tag="VFE39_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HET6258583.1"
                     /translation="MPVPVPVAQPSPVPSAGTHPGRWGRVDEEGTIYVHTPDGERVVG
                     NWQAGAPEEGLAHFARRFDDLLTEVELLAARLAAGGGEPKHALHSAKTLRDGLDEATV
                     VGDLVGLRARLDDLVVQAEEAVAAARSARDAQRAAAVARKEELAAEAEVLAAEATQWK
                     QAGDRFKAILDEWRAIRGIDRKTDELLWKRFSKAREAFNRRRGSHFADLDRQRATAKA
                     RKEELVVEAEKLTDSDDWGPTAARFRDLMTEWKAAGRAQKDADDGLWHRFRIAQDAFF
                     ARRAGTFAERDAEFTSNAEAKRALLVEAEKIDLSDPAGARAALRHIQERWEEIGKVPR
                     DDIRSLEAGLRAVEEKVREAADRQWRRTDPEAEARVAQFRERVAQFEAQAEKARAAGD
                     ARRAQQAQSQAEQWREWLTTAEQAVRQL"
     gene            12537..13265
                     /locus_tag="VFE39_08030"
     CDS             12537..13265
                     /locus_tag="VFE39_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258584.1"
                     /translation="MLSLVAVLPEPPLLVPELATGGAAETAELRSACVGAAARLAGAA
                     PRWIAVGADTGGRRTVCPPARGSFLGFGVDVVVGLDPASAAEPVDPDMPLPLLIAGWV
                     AAATGAGVSIRGELVAPDETPRACADLGAALAAECAADPGPVGLLVVGDGAATHSEKA
                     PGYLDERAGPFDAAVAAALRDADGGALAHLDVELATALFAAGRAPWQVLAGAAAGSRW
                     RGDLLHSSIPYGVAYHVAVWTRLT"
     gene            13262..14182
                     /gene="miaA"
                     /locus_tag="VFE39_08035"
     CDS             13262..14182
                     /gene="miaA"
                     /locus_tag="VFE39_08035"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET6258585.1"
                     /translation="MSGPPLVAVVGPTATGKSELGLALATDLGGEVVNADAMQLYRGL
                     DIGTAKLTPSERAGVPHHLLDVLDITEVASVAAYQRAAREVVERLRAGGRTPVLVGGS
                     GLYVQAVIDELEFPGTDPDLRAGLQAELAAGGSEALHARLAEVDPAAAEVVLPTNGRR
                     IVRALEVIALTGRPFPARLPTGGAARYDAVLLGVDRDTADLDARVARRVARMFAAGLV
                     DETRELLAHGLREGLTASRALGYQQVVAALDGVGDLGTAAAATVTATRRFVRRQRSWF
                     RRDPRIQWLDGGAPDLLVRARAVTSRAVGF"
     gene            14184..15059
                     /gene="dapF"
                     /locus_tag="VFE39_08040"
     CDS             14184..15059
                     /gene="dapF"
                     /locus_tag="VFE39_08040"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HET6258586.1"
                     /translation="MQFSKGHGTENDFVVLPDPDGVLDLTPARVAALCDRRRGLGADG
                     VLRVVRWAALGDGPEPEPGVEWFMDYRNADGTVAEMCGNGVRVFARYLEHAGWLTGGE
                     TPLGTRAGLRTVRLEPGDGTDGDVSVDMGPALVGETSTASIHGEAFPGVAVDVGNPHL
                     ACVTDVAIDGLDLTCPPGHDPALFPHGVNVEFVSPVSTSGGHAEIAMRVHERGVGETR
                     SCGTGTVAAVVAALRHAGRSRGTVAVHTRGGRLRVTVEGSTTVLHGPAVLVAHGELTR
                     SWWDAAAREPASVGC"
     gene            15171..16625
                     /gene="hflX"
                     /locus_tag="VFE39_08045"
     CDS             15171..16625
                     /gene="hflX"
                     /locus_tag="VFE39_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019816431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET6258587.1"
                     /translation="MTEPALSTTADDTRRATRGDYELEERTSLRRVAGLSTELTDVTE
                     VEYRQLRLERVVLVGVWTEGSSEQARASLTELARLAETAGSQVLDGLVQRRPRPDPAT
                     FIGTGKVDELRDAVLSAGADTVICDGELSPGQLRQLEEKLKVKVIDRTALILDIFAQH
                     ARSKDGKAQVELAQLSYLLPRLRGWGEALSRQVGGRAAGGVGIGGRGPGETKIELDRR
                     RIRTRMSKLRREISGMKQVRETQRGSRRRHEIPSVAIVGYTNAGKSSLLNSLTGAGVL
                     VEDALFATLDPTTRRTSTSDGRTYTLTDTVGFVRHLPHQLVEAFRSTLEETAGADLLV
                     HVVDASDALPEDQIKAVRQVLVEIGEQNAGTMPQELLVVNKTDAADDLQLARLRHLLP
                     DAVFVSARRGDGVQKLRERIAELLPRPEVDVELLLPYAEGSLVARVHSEGEVLAEEHT
                     PQGTRLHARVGAELATAVLPFALVGMPAEDGAAR"
BASE COUNT         2236 a         5841 c         6238 g         2427 t
ORIGIN      
        1 cgtcgccgcg ccccggctca gcccggacgg gcggttgctc gcctggctgc agtggaacca
       61 tccgtcgatg ccgtgggacg acacggagct ggtggtccgc gacctcggca cgggggaggt
      121 gacggtcgtg gccggcggcc cgggcgagtc ggtcgccgag ccgcggtggc agccggacgg
      181 gtcgctgtgg ttcatcagcg atcgcaccga ctggtggaac ctctaccgct gggtccccga
      241 cgcggacatc gagccggtcg tccggaccga cgcggagatc ggcgtgccgg gctggacctt
      301 cggctcggcg cgtttcgcgg tgctcgccga cgggcgggtg gtgttcgcgc ggcgccggca
      361 gggtttcgac gctctcgcgg tgcgcgagct cgacggctcc ctcgtcgacc tggatctgcc
      421 gttctcggcg atcagcgcgg tgcggtccac tggtcccggc accgtcgccg tcgtggcggg
      481 aagccccgct gccgagtcag gggtgttcct cgtggacgtg gccacctccg ccgtcgagcc
      541 gttgcggcca ccacgcgacc tccgcgtcga cccctcgatg gtgtcggtgc ccgaggcgat
      601 ctcgttcccg tcggtggacg ccgcgggtga acggcgaacc gcccacgcgc tgttctaccc
      661 acccacccac gagcagttcc gcggccccga cggcgagctc ccgcccctgc tcgtggagat
      721 ccacggcgga ccgacgtcgg cggccgtgcc ggcgctgagc ctccccgtcc agtactggac
      781 gagccgcggg ttcgcggtcg tggacgtgaa ctacggcggc tccaccggat acggccgtgc
      841 ctaccgcgag cagctgctgg gggagtgggg cgtggtcgac gtcgccgact gcatggccgc
      901 ggcggagtgg ctggcggcgc agggccgcgt cgaccggaac cggatgtgca tccgcggcgg
      961 ctcggcgggc ggctacacga cgctcgcggc gctggcccga gccgacaccc cgttcgcggc
     1021 tggagcgaac tactacggtg tggccgacct cgcggccttg gcggccgaca cgcacaagtt
     1081 cgagagccgt tacctcgacc gcctcgtcgg cccgtacccg gaagcccgcg aggtgtacga
     1141 ggagcgctcg cccatacacc atgtcgatcg cttcgaccgc ccgctgatcg tcctgcaggg
     1201 cgccgaggac gagatcgtcc cgccgagcca gagcgagctg atcgtcgacg cgctgcgggc
     1261 gcgccggcga cccgtggcct acctgctgtt cgacggggag cagcacgggt tccggcgcgc
     1321 cgagaacatc cgcagggcgc tggacgcgga gctcagcttc tacgcccagg tgctcgactt
     1381 cgagctgccg gcggacgagg gcatcgagcc ggtgacggtg gagaacctct gaccacgggt
     1441 cacccgccgc cggtggccgc agtgtccagc gtcgccgggt cggcgggtgc cgctgcggtc
     1501 cgcttcgccc gcgagagcct gcgctccacg tactcggcca gcttgccgag gccgtagttg
     1561 atgacgatga agatcagcgc cacgatcgcg taggtctgga tggggttgtg cagcgtctgg
     1621 atgatgatgt tcgccgtccg gacgaactcc tcgtagctga tgatgaagcc cagtgaggtg
     1681 tccttgacga tcaccacgag ctggctgatc agcgcgggca gcatcacccg gaacgcctgc
     1741 ggcagcagga tcgaggagag cacctgaccc cgggtcagcc ccagggagta gcccgcctcg
     1801 gcctgaccgc gcggcagcga cgccacaccg gcccggatga tctccgcgat gatcaccgag
     1861 ttgtaggcgg tgagcccgat gaccagatac cacaggatcg gcaggtccag gcccacctcg
     1921 ggcagcaccc gtgccgcgaa gaagatcgcg atgaccaccg ggacgccacg caggaactcg
     1981 atcacgccga cgaccagcca gcggtagtac cggttcgccg tgatgcggct gacagcgaag
     2041 agcgtgccga tcaccagcga gaagaccagc gtgagccctg cggcgaccag cgtggcgacc
     2101 agagcctgtc cgagcagccg ccagaccgcc aggaagctgg ggtcgaaggg gttgaccatg
     2161 ggtccccacc gttcggcgga gaactggccc tggctcgcca gccggatgat cacgagcgcg
     2221 atcacgacca gcacgatcac gctggcgacg ccactggaga tcagcgtgcg gcgccgggtg
     2281 acgggacccg gtgcgtcgta gaggaccgag gtgctcatcg ggccaccgcc accttccgct
     2341 cgaggacgcc cagcagaatg ccgccgggga tggtcagcag caggtagccc aggagcgcgc
     2401 cggtcagggc gcccaccgcg gcgtagccct gggccgaggt gagctggacg ccgttggcga
     2461 acaggtcgcc gccgatcccg aaggctccgg cgatcgccga gttcttgatc atggcgatga
     2521 tcacgctgcc gagcgggggc accacagtgc gcagcgcctg cggcagcacg atcagcgaga
     2581 gcgactggct gaaggtcagc ccgatcgcgc gggccgcctc ggcctgaccg gcggagaccg
     2641 agttgatgcc gctgcggacc gcttcgcaga cgaaggccgc cgtgtagcag gtcagcccga
     2701 tgacgccgaa gtagaagtag gacccgttga tgcccagctc aggcaggccg aacgccacag
     2761 cgaaaaggac gacagccagc ggggtgttgc ggaagaccgt gacccaggcc gtgccgatca
     2821 tccgtagcgg gggcaccggg gagacgcgga agccggccag gatcgtgccc agcacgagtg
     2881 caccgaccgc ggagaacacg cagagccgca ccgttccgag gaaacccagt acgaagagcg
     2941 ggaactgctc gatgatgacg cccacaacac ctctatcgga gacagactga ccggggtgcc
     3001 gaccggactg gtcggcaccc cggttgcggc gagatcagct gcaggtgccg ggtgcgggca
     3061 gcggcggaac ctccgggctg acctttccga cggtcgagtc ccacgcagcc ttgtagctgc
     3121 cgtcggcctc cgcctccttg agcgtctggt tgatgaactc gcagaaggcg acgtcgccct
     3181 tcttgatacc gatgccgtag ggctcctcgg tgaacttctg gttcacgagc ttgaacgccc
     3241 cgcccgagtt ctcgatcagg ccggtaagga tgacgttgtc ggtggtgacg acgtcgacct
     3301 gaccggtgcg cagcgcgtcg gcgcacttcg agtacacgtc gaacagcgtg aggttcgccg
     3361 ggtcgatgta cttcttgatg ttctcggaag gggtcgagcc ggccacggag cagaccttgg
     3421 cgttcgcgcc gcgcagcgag tccggaccgg tgatggtggt gttgtcgctc ttgaccatca
     3481 ggtcctggcc cgcgacgtag tacgggccgg cgaagctgat ccgctccttg cgcttgtcgt
     3541 tgatcgtgta ggtggcggcg accatgtcga cgcggccctg ctcgatcact tcctcacgca
     3601 cggcggacgg cgcctcggtg taggcgatct tgtcggcagg gatgccgagc ttggccgcga
     3661 tgatcttggc gatctcgacg tcgaagcccg ccgggttgcc gtcgagaccc ttcaggccga
     3721 acagtggctg gtcgaacttg gtgcccagcc gcaaagtctg ggccttgttg agcgtgtcca
     3781 tcgtgctgcc ggccgggaag gacgcgccct gggccacgga cggtgcagct cccgaggaac
     3841 cgagcgatga ggactggtca ccacaggcgg tggcggcgat caccacggtc gccgcgacgg
     3901 cggcgatccg gatgaggtga gagaaacgca tggtggtgcg ggtccttccg tcggtgcggg
     3961 aaaacggggc cggtgcgctt ccggcgtgcg ggccgtcagt gcgtgaggat cttgccgagg
     4021 aagtccttgg cgcggtccgt ggtgggattg gagaagaacg tctccgggct cgcgtcctcg
     4081 acgatctcgc cgtcggacat gaacaccacc cggtgggcgg cccggcgggc gaagcccatc
     4141 tcgtgggtca ccacgagcat ggtcatgccc tccccggcca atgcggtcat cacgtcgagg
     4201 acctcttgga ccatctcggg gtcgagggcg gaggtcggtt cgtcgaagag catcaccttc
     4261 ggcttcatgg cgagtgcgcg ggcgatcgcg gcccgctgct gctggccgcc ggagagctgg
     4321 gccgggtact tgtccttctg gttcgcgatc cccacccgct cgaggagtgc gagaccgtcc
     4381 ttcccggcga cggccttgcc caccttgcgt accttgagcg gcccgagcgt gacgttctcg
     4441 aggatcgtct tgtgcgcgaa gaggttgaag ctctggaaca ccatgccgac atcggcacgc
     4501 agccccgcga gggccttgcc ctcttcgggc aggcgcttgc cgtcgacctc gatggtgccg
     4561 ctgtcgatgg gttccagccg gttgatcgtc ctgcacagcg tcgatttgcc ggaccccgac
     4621 gggcccagca ccaccacgac ctggccgctc tcgacctcga ggttgatgtc gcgaaggacg
     4681 tgcagctcgc cgaagtgctt gtcgacggac gccatccgga tcatgggagt gttggtcatc
     4741 gggggcctcc gtgcatggcg ggacgctagg acgaatcgga cggctcagtc cagcaccttg
     4801 agcaacccat agggtttcgc tccgataacg ctcggcatgg aggcatgagg acgtgcacat
     4861 ccttctggtg gaggacgacg accgcatcgc ggccgcgctg cgcccggcac tgcaccggaa
     4921 cgggatgaca acgacgcggc tctcacgcgg acgtggagct gtcgaccacc tggccggcgt
     4981 cgatgtcgtg ctgctcgatc tcggcctccc cgacatcgat ggcgtcgacg tgtgcagggc
     5041 gatccgcgag gtcagcgagg tgccggtgat cgtggtgtcg gcgcgcggcg aggtcgacga
     5101 ccgcatcctc ggcctgcatt ccggcgccga cgactacctt gtcaagccct acgacatcgg
     5161 tgagctcgtg gcgcgcgtgc acgcggtgta ccggcggcgc cgggtggtgt cgtcggccgg
     5221 accggaggac gtcgtggtgg tcgacaacgt gagcgtcgac ctgcaccgcc actccgtcac
     5281 ggtcgggggc aggccggtca acctcaccag gaaggagttc caggtgctcg cgctgctcgc
     5341 cagctctggg ggcgcggtgt gcacgcgcag ccggatcgtg gcggaggtgt ggggccggtc
     5401 ctgggccggc gcgaatcgca cactcgacgt gcacgtggcc acgctgcgca ccaagctcgg
     5461 ccgcgccgac ctcgtgcaaa ccgtccgggg cgtgggctac cggctcggac tgcccgagag
     5521 cggcaacccc gacagtgtca tcccgaacag cggcgtcccc gacaacggta cggagtagga
     5581 gtgcgcaccc ggctgctggt gatcgtgctg ttcctggtcg gcctgctggc cgtcgggctc
     5641 ggcgtgccgc tggcgctgag caacgcgcag gcgaaccagc tcgagctgtt caccgaccgg
     5701 ctgaccgaca cgatcttcta cgcctcgctg gccgagcgcc cgatcaccga ggcggacacc
     5761 acggggctgg agcaggagct ggcccgctac gacgccgtct acggcgtggc ggtgatggtg
     5821 gtggaccgca acgggcagct ggtcgccacg tcgcgacacc caccccccgt gctcgacgag
     5881 gccggccgcg agcgggtcga gctggcgctg gcgaaccggc gctcggaggc ctacccgccg
     5941 ttgctgccat gggacgagcg gccgctggtg ctcgccgagc cggtgctggt cgacaccgag
     6001 gtccgcggcg ccaccatcac ggtgtcgccg acggccgcgc tgcgcgcaga ggaggtgcgg
     6061 gtgtggtcgc tggtcgcggc cgccgggctg ctggccctcg cgatggccgt gctggtcgcg
     6121 ctgccgatcg tccggtggat cctgcgcccg gtccgcaggc tcgacgaggg caccgggcgg
     6181 gtcgcggcgg cggtgctcgc cggggccggg gcggagccgg ttgccgacgg gtccgggccg
     6241 cccgagctgc gcaggctgtc ggtctcgttc gaccggatgg ccgagacggt gacgcaggcc
     6301 tacgcggcgc agcgcgcgtt cgtcgccgac gcgagccacc agctgcgcaa cccactcacc
     6361 gcgctgcgcc tgcggctgtc caacctggag ggccacgtgg acgggtccgc catcgaggac
     6421 cacatggccg cgctggagga ggcggatcgg ctctccacgc tgctggacgg gctgctcgcg
     6481 ctggcccgcg ccgagcgcac cgcaccagtg gtcgtcgtcg aggtggatcc tgcggtggcg
     6541 gaccggatcg acgcctggcg gcccctggcc gagcacacgg gcctacagct cgtccgttgc
     6601 gggcggcgcg ggttgcgggc ggcggcgtcg gccggggcga tcgagacggt gctcgacgcg
     6661 gtgctggaca acgcggtgaa gttctccccg ccgggcggca cggtgaccgt tcgcacgggg
     6721 acgacgccga cgcacgtgga ggtcgcggtg cgcgacacgg gcccgggcct cgcccctgag
     6781 gagctggagc gggccaccga ccggttctgg cgcagcccgg cgcagagcaa cgtcgatggc
     6841 tccgggctgg ggctggcgat cgccgcccgc actgtcgagc tcgccggtgg ggagctgcgg
     6901 ttggagctgc ccgcgggcgg tgggctgcag gtgatcgcgc tgcttcccca agtgccgggc
     6961 gaggagccgg acggggagca gccggtggtc gaacgggagg ccggcatgcc gtcctcgccc
     7021 gcctgacgtc cggcggaacg gtccgaccct cccggcccgg acccggaccg gtgggaatct
     7081 gccgggctcg gtgacccgcg gatccggaca gtcactagcc gttcttcaat gcgcggaaga
     7141 accgctctgc gccggggtgc agcggcaccg gctgggtgcc gatcgccgct cgggggtcga
     7201 tggtgagcgc cgaggggctc gccaccgcca gctccggctg ggcgtcgaac agaccctcga
     7261 cgagcgcgct cgcgagatcg tccggcatcg aggccggcac cagcaggaag ttgcgcacga
     7321 gcagggtcgt gatgggctcg gggatgccgt atgtcgcggc aggcacggtg ccggaggcgt
     7381 acgccgggta ggccgtatgg acggcggtga tcatgtcctc gaggttcagc aatcggatcg
     7441 gcactgtcct cgccagccgg ctgaccccgt cggtgggcac cccgccggac cagaagaacg
     7501 catcgatcgc gccactctcc atggcggcca cggactcgtt gatgccgagc tgctcggcct
     7561 gcaggtccgt gtccggcgac accccggcca accccagcag ccgcgtggcg atcacataca
     7621 cgcccgagtc gggagcgccg atcgacaccc tggctccccg caggccggcc agagtcgtca
     7681 ccggggacgt ggccctgacg acgacgtgta cggcgtcgtc gtagatccgg gcgagcgcac
     7741 gaggggcgcg ctggtcgccc ggggccgact tcaacagctg gtcggccgcg gtatccacct
     7801 ggctgaacgc cacgtccacc gctcctgtgg cgagcagtcg caggttgtcc accgagccgg
     7861 ccgtcgccag cacctcggga cggacgtcga gcccgagctt ccgctgccac gcgtccgcca
     7921 gggcgtttcc gagcccgaag tagacgccct gggtgccgcc ggtcgcgagc gtcagccggg
     7981 tgctggcgta gggggacgag cagccgccga gcgcggcgag caggccgagc aggccgagcc
     8041 cgcccagcac cgcgcgccgc ccgcacgagc cgccgccctc cacggggggc atcctgccgt
     8101 gcccggccga tgcggtcgac atcgtgccga cggcctaccc tcattccgtg ggccgtagct
     8161 acaccatccg cacctacggg tgccagatga acgtgcatga ttccgagcgg atggctgggc
     8221 tcctggaggc ggcgggatac acgaaggccg actcggagga cgcggacgtc gtcgtgttca
     8281 acacctgcgc cgtccgcgag aacgccgaca accggctcta cggcaacctc ggccacctcc
     8341 ggccggtgaa gaccgcccgg ccgggaatgc agatcgcggt gggcggctgc ctcgcgcaga
     8401 aggaccgctc cgagatcgtt cggcgcgctc catgggtcga tgtggtcttc ggcacccaca
     8461 acgtgcacgc cctgcccgtc ctgctggagc gcgcacgcca caacgccacc gctcaggtgg
     8521 agatcgccga gtcgctggag gtcttcccct ccacgctgcc ggcgcgccgc gagtcggcct
     8581 acgccggctg ggtgtcgatc tccgtgggct gcaacaacac gtgcaccttc tgcatcgtgc
     8641 cgtcgctgcg gggcacggag aaggaccgca ggccgggcga cgtcctggcc gaggtgtccg
     8701 cgctggcggc cgacggcgtg ctcgaggtga ccctgctcgg gcagaacgtc aactcctacg
     8761 gtgtcgagtt cggcgaccgc ggcgccttcg cgaagctgct gcgggcatgc ggcgacgtcg
     8821 agggcctcga acgcgtgcgg ttcacctcgc cgcacccccg cgacttcacc tccgacgtga
     8881 tcgcggcaat ggccgagacg ccgaacgtgt gcccccagct gcatatgcct ctgcagtccg
     8941 gctcggacga cgtgctgcgg cggatgcgcc gctcctaccg gtccgcgcgc ttcctggcga
     9001 tcatcgacga ggtgcgcgcc gcgatcccgc acgccgcgat cagcaccgac atcatcgtcg
     9061 gcttcccggg cgagaccgac gccgacttcg aggccaccct cgatgtggtg cgctccgccc
     9121 gcttcgcgaa cgcgttcact ttccagtact cccttcggcc cggcacgccc gctgcggcgc
     9181 tgcccgacca actgcccaag gccgtggtcc aggagcggta cgagcggctc gtcgcgctac
     9241 aggaggagat ctcctggtcg gccaaccgcg cgctcgaggg gcaggtcgtc gaggtactgg
     9301 tgtcgacggg ggaggggcgc aaggacagcg ccacccggcg gatgagcggc cgcgctcgcg
     9361 acgggcggct tgtgcacttc gctcccggcc cggcagcgtc ggacgccggt gggatccgtc
     9421 ccggtgacat cgtcgaggtc gccgtcgggt atggggcgcc gcaccatctc gtggccgacg
     9481 gcccggtcct gtcccaccgg cgcacgggtg cgggcgacgt ccacgagatg ggtacccggc
     9541 ccgggggtgt gccgaccggc ctgggcctgc cgtccttcgg cgcgccggtg caggcggcgg
     9601 ccacgcccgg gtgtgcgacg agcgcatgag cgccccggcg ggtgacggag tgcagcgagg
     9661 gagagcgatg aatgagcgga aggtccccga ggagctggcg gctctcgacg cggagctgac
     9721 cggaatcggt cggcgcgtcc agcgccagat cgaccccggc ccggcggcgc tgggcgtgtc
     9781 catcgcgata ctcgtgctga tcgtggcact gatcctgccg tggaccggct cggtgcgcgg
     9841 ttgggaggtg ctcgcgggcc tcgagccgcg gctcggggtg ctgccgcggc tgttcacctt
     9901 tacctcgctg ggcttcggcc tggtggtctc gacgctcgcg ctcgccaccc ggtggtgggc
     9961 gctggcctgg ctgtcggcgg tggggtgcgg gatctcggtg gtcacggggc tgtgggcgat
    10021 ctggtcccgg caggtcgcgg tgccggtcgg gggcaccggg cccggtgtcg gggtcgtgct
    10081 ggccctcctc gcggtgatca cactcgccgt ctgttgggcg cggatcgccg gtcgccgagg
    10141 gtgaaaggat ctggcgtcgg tgagaccgac gatcggagac atcgccgcct gacggcggcg
    10201 cccgcacggt tccgctgatc accggaactg tgcccgcacg gtcccttcga cgactgtgag
    10261 ggcacgatac cggtgatcat cctcagagtt gccggaccgc ctgttccgcc gttgtcagcc
    10321 actcgcgcca ctgctcggcc tggctctggg cctgctgggc gcgccgggcg tctccggcag
    10381 cgcgggcctt ctccgcctgc gcctcgaact gggccacccg ctcgcggaac tgggccaccc
    10441 gcgcttcggc ctcgggatcg gtgcggcgcc actggcggtc ggcggcctcg cgcaccttct
    10501 cctcgacggc gcgcaggccg gcctcgagcg accggatgtc gtcgcgcggg accttgccga
    10561 tctcctccca gcgttcctgg atgtggcgca gggcggcgcg cgccccggcc ggatcggaca
    10621 ggtcgatctt ctcggcctcc acgagcaggg cccgcttcgc ctcggcgttc gaggtgaact
    10681 cggcgtcgcg ctccgcgaag gtgccggcgc gccgcgcgaa gaacgcgtcc tgcgcgatgc
    10741 ggaagcgatg ccacaggccg tcgtcggcgt ccttctgagc gcggcccgcg gccttccatt
    10801 ccgtcatcag gtcgcggaag cgggccgcgg tggggcccca gtcgtcggaa tcggtgagct
    10861 tctccgcctc gacgaccagc tcctccttcc gcgccttggc cgtggctcgt tggcggtcga
    10921 ggtcggcgaa atgcgatccg cggcggcggt tgaacgcctc gcgcgccttc gagaaccgct
    10981 tccacagcag ttcgtcggtc ttgcggtcga tgccgcggat ggcccgccac tcgtcgagga
    11041 tcgccttgaa ccggtcgccg gcctgcttcc actgcgtggc ctccgcggcg agcacctcgg
    11101 cctcggcggc gagctcctcc ttgcgggcta ccgcggcggc ccgctgggcg tcccgcgcgg
    11161 accgggccgc ggcgacggcc tcctccgcct gcaccaccag gtcgtccaac cgcgcgcgca
    11221 gcccgaccag gtcgccgacc acggtggcct cgtccagtcc gtcacgcagc gtcttcgcgc
    11281 tgtgcaaggc gtgcttcggt tcgccgcctc ccgcggcgag acgggcggcg agcagctcga
    11341 cctcggtgag caggtcgtcg aaccggcggg cgaaatgggc gagcccctct tcgggcgccc
    11401 cggcctgcca gttacccacg acgcgttcgc cgtcgggtgt gtggacgtag atcgtgccct
    11461 cctcgtcgac acggccccag cggcccgggt gggtgcctgc gctcgggacg ggggagggct
    11521 gggcgacggg cacgggcacg ggcacgtccg gcggggcagc cgcctgcgcc gcaggtgcgg
    11581 ggtgcggcgc ggccgggacg gcgaccggcc ccggtcgggg cgcggcgggt accgcgggcg
    11641 gcccgggtcg cggtggcgcc gggacgacgg gtggcccagg ccgaggaggg ccgggaaccg
    11701 gggccgtggc ggtggtgtcc gggacgggtg attcctggtg gtcggaggcg gtgtcgggcg
    11761 catcgctgcc cggcggcgtg ggagcggtgg cgggcccggc gtcggtcggg ggtgcggggg
    11821 cggcgtcggg cgcatcggtg gccggagccg cgggggcggt gtcgggctcg ctggtggcag
    11881 gcgccgcggg ggcctcgtcg gtcggcggct cgacccccgt ggtggcgctg tccgcgctgg
    11941 aacctcgggc gtcgggggag gggccgggtg gcggggtgtc acgggcgggg acagggtgcg
    12001 cggcgtgggc cgcagccagc gggcccgcct ccgccagcgg aggggtggcc gctgcctcga
    12061 cgtcggagcc cggctcggcc gcagcgccgg accggtggtg gcgtccgagt tccagcggtg
    12121 aaggccgggg gaccggggtc tcctcggcct cagcgcctgc gacagcgggg tcgccggcgg
    12181 ccggctgcgc cggctcctgg cccgtcggct gcttcccttc cgcctgctcg accgcacggt
    12241 cggcgtcgac cgtcccgccc tcggtggccg tggctggcgt tcccgtaccc tgttgctccg
    12301 acaccgtcgt cctccttgct caggcccacc gcgagcggtg ggcgccccgc ccgggggcgg
    12361 agccgtgcac agcgaccgtc gccagtcctc gtgaccagtg gtcgtccagc gattcaaaca
    12421 gctccagggt cggaccggaa caacccgcat gggcgctgcg cagccagcgg ccggtccggg
    12481 tcggtgagcc gcgcccgaac acgctggtgg gagccccgcg gcatacgctg agatgcgtgc
    12541 tgtcgttggt cgccgtgcta cccgagccac cgctgctcgt ccccgaactg gcgacgggcg
    12601 gcgcggccga gaccgccgag cttcgctcag cctgtgtcgg cgccgccgcg cggttggccg
    12661 gggccgcgcc gcggtggatc gccgtgggcg ccgacaccgg cgggcggcgc acggtctgcc
    12721 cgcccgcccg gggcagcttc ctcggcttcg gcgtggacgt cgtggtgggg ctcgacccgg
    12781 cctcggccgc tgagcccgtc gaccccgaca tgccgcttcc gctgctgatc gccggctggg
    12841 tggcggccgc cactggcgcc ggtgtcagca tccggggtga gctcgtggcc cccgatgaga
    12901 caccgcgggc gtgcgccgac ctcggtgccg cgctcgccgc ggagtgcgcg gcagaccccg
    12961 ggccggtggg tctgctggtc gtgggggacg gcgccgcgac gcacagcgag aaggccccgg
    13021 gctacctgga cgagcgtgca ggaccgttcg acgcggccgt tgccgcggca ctccgggacg
    13081 ccgacggcgg cgcactggcg catctggacg tcgagctcgc caccgcactg ttcgccgcgg
    13141 gacgggcacc gtggcaggtt ctcgccggag ccgccgcggg gagccggtgg cgcggcgacc
    13201 tcctgcactc ctcgatcccg tacggcgtgg cctaccacgt ggccgtgtgg acgcggctca
    13261 cgtgagcgga ccaccactgg tcgccgtcgt gggccccacg gcgaccggca agtccgagct
    13321 gggcctcgcg ctggcgacgg atctgggcgg cgaggtcgtc aacgccgacg cgatgcagct
    13381 ctaccgcgga ctcgacatcg gcacagccaa gctcacgccg agcgaacggg ccggggtccc
    13441 gcaccacctg ctcgatgtcc tcgacatcac ggaggtggca tcagtggcgg cctaccagcg
    13501 cgcggcgcgg gaggtggtgg agcggctgcg agcaggcggc cggaccccgg tgctcgtcgg
    13561 cggatccggt ctgtacgtgc aggccgtcat cgacgagctc gagttccccg gaacggaccc
    13621 tgacctgcgt gcggggctgc aggcggagtt ggccgcgggc ggatccgaag cgctgcatgc
    13681 acggctcgcc gaggtcgacc cggcggccgc cgaggtggtg ttgccgacga acggccgacg
    13741 catcgtccgt gcgctcgagg tgatcgcgct gaccgggcgt ccgttcccgg cacggctgcc
    13801 caccggcgga gcggcgcgct acgacgcggt gctgctcggt gtcgacaggg acacagcgga
    13861 cctcgacgct cgggtcgccc ggcgggtcgc gcggatgttc gcagccgggc tcgtcgacga
    13921 gacccgcgag ctgctcgccc acgggctgcg tgaggggctc accgcgtcgc gcgcactggg
    13981 ctaccagcag gtcgtcgctg cgctcgacgg cgtcggggat ctcgggaccg ccgcggcagc
    14041 gacggtgacc gccacgcggc ggttcgtgcg ccgccagcgc tcctggttcc gccgcgaccc
    14101 gcgcatacag tggctcgacg gcggcgcgcc cgatctcctc gtccgggcca gggccgtgac
    14161 ctcgcgggca gtaggcttct gacgtgcagt tcagcaaggg acacggtacg gagaacgact
    14221 tcgtcgtgct ccctgacccg gacggcgtcc tggacctcac cccggcgagg gtggccgcgc
    14281 tgtgcgatcg caggcgcggc ctgggcgcgg atggcgtgtt gcgggtggtg cgctgggcgg
    14341 cgctcgggga cgggccggag cccgagcccg gtgtcgagtg gttcatggac taccgcaacg
    14401 ccgacggcac ggtcgccgag atgtgcggca acggggttcg ggtgttcgct cgctacctcg
    14461 agcacgcggg atggctcacc ggcggcgaga cgccgctggg cacgcgcgcc ggcttgcgta
    14521 cggtgcgtct ggaaccgggg gacggcaccg acggtgacgt ctccgtcgac atggggcccg
    14581 cgctcgtcgg ggagaccagc accgccagca tccacgggga ggcgttcccc ggcgtcgcgg
    14641 tggacgtcgg taacccgcac ctggcgtgtg tcaccgacgt cgccatcgac gggctcgatc
    14701 tgacctgccc gcccgggcac gacccggcgc tgttcccgca cggggtgaac gtcgagttcg
    14761 tcagcccggt gtcgacgtcg ggagggcacg ccgagatcgc gatgcgcgtg cacgagcgcg
    14821 gtgtggggga gacacggtcg tgtggcaccg gcacggtggc ggccgtcgtg gccgcgctgc
    14881 gacacgccgg tcggagcagg ggcacggtcg ccgtgcacac ccgcggcggg cgactgcgcg
    14941 tcacggtcga gggctcgacg acggtcctgc acgggcccgc cgtgctcgtg gcccacggcg
    15001 agctgacccg ttcctggtgg gacgccgcag cgcgggagcc cgcttcggtc ggctgctgag
    15061 ctgccggtcc gacaccacgg cgcgcggacc cgagcgactc gcgaggccgc taccacccgc
    15121 cgaaacaaac cagacagcgg gggcgttgac atgggagcat gaacctcaca atgactgaac
    15181 ccgccctgtc caccacagct gacgacacgc gccgcgccac ccgtggcgac tacgagctcg
    15241 aggagcgcac gtcgttgcgc cgcgtcgccg ggctgtccac cgagctgacc gacgtcaccg
    15301 aggtcgagta ccggcaactg cgcctcgagc gcgtcgtact cgtcggcgtc tggaccgaag
    15361 gcagctccga gcaggcccgg gcctccctca ccgagctggc gcgtctcgcc gagaccgccg
    15421 gttcccaggt gctcgacggt ctggtccagc gccgcccccg gcccgacccc gccacgttca
    15481 tcggcacggg caaggtcgac gagctgcgcg acgccgtgct ctcggccggc gccgacaccg
    15541 tgatctgcga cggcgagctg tctcccgggc agctgcgcca gctggaggag aagctcaagg
    15601 tcaaggtgat cgaccgcaca gcgttgatcc tcgacatctt cgcccagcac gcccgctcca
    15661 aggacggcaa ggcgcaggtc gagctggctc agctgtccta cctgctgccg cgcctgcgcg
    15721 gctggggtga ggcattgtcc cggcaggtcg gtggccgggc cgcaggtggc gtcgggatcg
    15781 gcggccgcgg ccccggtgag accaagatcg agctcgaccg caggcgcatc cgcacccgga
    15841 tgtccaagct gcggcgcgag atctccggga tgaagcaggt gcgcgagacc cagcgcgggt
    15901 cacggcgtcg tcacgagatc ccgtcggtcg cgatcgtcgg ctacaccaac gcgggaaagt
    15961 cgagtctgct caactcgctc accggcgcgg gcgtgctcgt cgaggacgcg ttgttcgcca
    16021 ccctcgaccc caccacccgg cgtacgtcca cctccgacgg gcgcacctac accctgaccg
    16081 acacggtcgg gttcgtgcgg cacctgccgc accagctcgt cgaggcgttc cggtccacgc
    16141 tggaggagac ggcgggcgcc gatctgctcg tgcacgtcgt cgacgcctcc gacgcgcttc
    16201 cggaggacca gatcaaggcc gtccgccagg tgctagtcga gatcggtgag cagaacgcgg
    16261 gcacgatgcc gcaggagctc ctcgtcgtca acaagaccga cgcggccgat gatctgcagc
    16321 tggcgcggct gcgccacctg ctgccggacg cggtgttcgt ctccgcacgg cgcggcgacg
    16381 gggtccagaa gctccgcgag cgcatcgccg agctgttgcc gcggcccgag gtcgacgtcg
    16441 agctgctgct gccctacgca gagggttcgc tggtggcgcg cgtgcactcc gagggtgagg
    16501 tactggccga ggagcacacc cctcagggca cccggttgca cgcccgcgtc ggcgctgagc
    16561 tcgccaccgc cgtgctgccg ttcgcgctgg tcgggatgcc cgccgaggac ggcgccgcac
    16621 gctgatccag ctcctcctgg ccggtgcgct cgtagcgtcg ctcgccggcg tgcctgctcc
    16681 ggaagcccgg tgtgccgtcg acgaccccag gctcacggag ctgtccgggc tcgtcgtcga
    16741 cg
//
LOCUS       DASRSC010000165         5054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4639574,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000165 DASRSC010000000
VERSION     DASRSC010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4639574"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1408)
                     /gene="pafA"
                     /locus_tag="VFE39_08050"
     CDS             complement(47..1408)
                     /gene="pafA"
                     /locus_tag="VFE39_08050"
                     /EC_number="6.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA];
                     GO_process: GO:0070490 - protein pupylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pup--protein ligase"
                     /protein_id="HET6258588.1"
                     /translation="MQRRIYGVETEFGVTCTFHGQRRLSPDEVARYLFRRVVSWGRSS
                     NVFLRNGARLYLDVGSHPEYATAECDSLTQLVAHDKAGERILEDLLVDAERRLVDEGI
                     GGDIYLFKNNTDSAGNSYGCHENYLVARAGEFSRIADVLLPFLVTRQLICGAGKVLQT
                     PRGAVYCLSQRAEHIWEGVSSATTRSRPIINTRDEPHADAERYRRLHVIVGDSNMSEV
                     TTLLKVGSATLVLEMIEAGVQFRDFTLDNPIRAIREISHDLTGRRTVRLAGGREASAL
                     DIQREYYSRAVEHVATRGSHDPTVQRVLDLWGRTLDAVESQNLSMIDREIDWAIKHRL
                     VERYRQRNDMDLANPRIAQIDLAYHDIRRGRGLFDLLQRKGMVDRVTDDGEIEAAKDT
                     PPQTTRAKLRGDFIAAAQAAGRDFTVDWVHLKLNDQAQRTVLCKDPFRSVDDRVEKLI
                     AGM"
     gene            1608..3152
                     /locus_tag="VFE39_08055"
     CDS             1608..3152
                     /locus_tag="VFE39_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET6258589.1"
                     /translation="MSWFRRAADEVNDADRAVSRAVSAMPPSPLDTAMKNLSTAANHS
                     LLWFALAALLASRRGASRQAAARALLAIAGASGTANALLKPALPRRRPPAAELPAYQT
                     IDHPPTSSSFPSGHAASAAAFATAVALESPRLGLVVAPLAASVAYSRVHVGVHWASDV
                     LAGAAVGSGVALATRRWWPVRRGDEARARPLDAVPELPRGEGLVIVSNQRSGDPEYDP
                     ANELETALPAAMVLRAEPDRDLDEQLDTAVAERDGWVRAIGVAGGDGTVAAGATVAGR
                     RNLPLVVVPTGTLNHFARDVGIYDLQEAVDATGAGEAVAVDLGLIDVHPGLGADPEIK
                     EVERVRCFLNTASLGSYPDLVRLREQWQPRWGKWPAFAAALVVVLHRAEPVEIKIDGR
                     WTSVWFLFVGNGPYHPRGMVPAWRPSLDSGLLDVRWLRADVRFSRLRAVVALLLGALG
                     HSRVYHQREVPVLDVELAVPGMLATDGEVVEEAGRYTFRVAKDAIPVYRRQEDRWTGR
                     DRPFVG"
     gene            complement(3253..3630)
                     /locus_tag="VFE39_08060"
     CDS             complement(3253..3630)
                     /locus_tag="VFE39_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6258590.1"
                     /translation="MSIELNHTIVAARDKNAEARFVTDVLGLPGPVPFGPFAVVELQN
                     GVSLDFMDTADVPSQHYAFLISEEEFDAVAGRLRDRGVPTYGDPGHQTPGHNTNDGGR
                     GLYFSSPDGHNLEVLTKPYGSGG"
     gene            complement(3695..4570)
                     /gene="prcA"
                     /locus_tag="VFE39_08065"
     CDS             complement(3695..4570)
                     /gene="prcA"
                     /locus_tag="VFE39_08065"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HET6258591.1"
                     /translation="MTMPFYSSVDQLLRDRSELARKGIARGRSVVVLTYTGGVLFVAE
                     NRSSENRPSALRKVSEIYDRIGFAAVGRYNEFETLRNAGIRMADVRGYSYDRRDVTGR
                     MLANYYAQVLGTSFVEQQKPYEVELCLAAVGETPEADQLYRITYDGSITDEPQFVVMG
                     GTTEPISAKLKDTYRPGMELAEAIGIAVEGLQATGTPAGSGPTGSNGGAQATRVLGAN
                     ALEVAVLDRSRPHRTFRRITGQVLRDLLPEENRAVDAPPPPTVAGDDVPAASGAVNGD
                     QEDQGDKPGGAANGS"
     gene            complement(4616..>5054)
                     /locus_tag="VFE39_08070"
     CDS             complement(4616..>5054)
                     /locus_tag="VFE39_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HET6258592.1"
                     /translation="ASMIQQNLGAALQGFVVVPLFVGYDLDAADPAKAGRIITYDATG
                     GRYDELLGYHAVGSGSVFAKSALKKLYDPAADRDLAVRTAVEALYDAADDDTATGGPD
                     LVRRIYPVVVTITADEGAVRRPEDEMETVVRAVVAGRMEKPGG"
BASE COUNT          671 a         1745 c         1836 g          802 t
ORIGIN      
        1 gcctgtgagc aggctgtggc gcggggagag tcgcgcccgc cgggtcctac attccggcga
       61 tcagcttctc cacgcggtcg tccaccgacc ggaaggggtc cttgcacagc acggtgcgct
      121 gcgcctggtc gttgagcttg aggtgcaccc agtcgacggt gaagtcgcgt cccgcggcct
      181 gggccgccgc gatgaagtcg ccgcgcagct tggcccgggt ggtctgcgga ggcgtgtcct
      241 tcgcggcctc gatctcccca tcgtcggtga cccggtcgac catgcccttg cgctggagca
      301 ggtcgaacag cccgcgcccg cgccggatgt cgtggtaggc gaggtcgatc tgggcgatgc
      361 gcgggttggc gaggtccatg tcgttgcgct ggcggtaccg ctcgacgagc cggtgcttga
      421 tcgcccagtc gatctcgcgg tcgatcattg acaggttctg cgactcgacg gcgtcgagcg
      481 tgcgccccca caggtcgagc acccgctgga ccgtcgggtc gtgggaaccg cgcgtggcga
      541 catgctcgac ggcgcggctg tagtactcgc gctggatgtc cagcgcgctc gcctcccgtc
      601 cgcccgcgag ccgcacggtg cgcctgccgg tcaggtcgtg gctgatctcg cggatcgcgc
      661 ggatcgggtt gtcgagggtg aagtcgcgga actggacgcc cgcctcgatc atctccagca
      721 ccagcgtggc cgagccgacc ttgagcagcg tggtgacctc ggacatgttc gagtcgccga
      781 cgatcacgtg cagcctgcgg taccgctcgg catcggcgtg gggctcgtcg cgcgtgttga
      841 tgatcggacg ggagcgggtg gtggccgagg agacaccctc ccagatgtgc tcggcgcgct
      901 gcgaaaggca gtacacggcg ccgcgagggg tctgcagcac cttgcccgcg ccgcagatca
      961 gctggcgcgt caccagaaag ggcagcagca cgtcggcgat gcgggagaac tcacctgccc
     1021 gcgcgaccag gtagttctcg tggcagccgt aggagttgcc cgccgagtcg gtgttgttct
     1081 tgaacaggta gatgtcgccg ccgatgcctt cgtcgacgag ccggcgctcg gcgtcgacga
     1141 ggaggtcctc gaggatccgc tccccggcct tgtcgtgcgc caccagttgg gtgagcgagt
     1201 cgcactccgc ggtggcgtac tcgggatggc tcccgacgtc gaggtagagc cgggcgccgt
     1261 tgcgcaggaa gacgttcgac gaacgtcccc acgacacgac ccggcggaac aagtaccggg
     1321 ccacctcgtc cggggagagg cgtcgctggc cgtgaaaggt gcaggtgacc ccgaactcgg
     1381 tctccacacc gtagatccgc cgctgcatcc cgtcagagta ggcggagaga ccccactcat
     1441 ggcacagctg tgaccgaccg gttcgggtac gtcgtcccgt agtttcgccg aagtcccctg
     1501 ccggttgggc cattcgcgtt gcttcacggg gccccgctgg gcctcgggga tgttcagcgg
     1561 gtcgccccgg cccagtcgga ggagcgcctg cgtagggtcg gtccgtgatg tcctggtttc
     1621 ggcgggccgc cgacgaggtg aacgacgccg accgcgccgt gtcgcgggcg gtctccgcga
     1681 tgcccccctc gccgctcgac accgcgatga agaatctgtc gaccgcggcc aaccacagcc
     1741 tgctgtggtt cgcgctcgct gcgctgctgg cgtcgcggcg aggggcgagc cgccaggcgg
     1801 ccgcccgtgc tctcctcgcg atcgcgggcg cgagtggcac ggcgaacgcg ctactcaagc
     1861 cggcgttgcc gcgccgcagg ccgcccgcgg cggagctccc ggcctaccag acgatcgatc
     1921 accccccgac ctcgtcgtcc ttcccgtccg ggcacgccgc gtcggcggct gcgtttgcca
     1981 ccgctgtggc tctggagagc ccgcggctcg gtctcgtcgt cgccccgctt gcggcgtcgg
     2041 tggcctactc gcgggtacac gtcggcgtgc attgggcctc ggacgtgctc gccggggcgg
     2101 ccgtgggcag tggtgtcgcg ctcgccaccc gccgttggtg gccggtgcgg cgcggcgacg
     2161 aggcccgggc ccggccgctc gacgcggtgc ccgagctccc ccgtggtgag ggcctcgtga
     2221 tcgtgtccaa ccagcgctcc ggtgaccccg agtacgaccc ggcgaacgag ctggagaccg
     2281 cattgcccgc ggcgatggtg ctgcgcgccg agcccgaccg cgacctggac gagcagctcg
     2341 acaccgcggt cgccgaacgg gacggctggg tccgggcgat cggcgtcgcg ggcggcgacg
     2401 gcacggtggc cgccggcgcc acggtggccg ggcgccgcaa cctgccactg gtggtggtgc
     2461 ccacggggac gctcaaccac ttcgcccgcg acgtcggcat ctacgacctg caggaagcgg
     2521 tggacgccac gggggcgggc gaggcggtcg ccgtggatct cgggctgatc gacgtgcatc
     2581 ccgggctcgg cgccgacccg gagatcaagg aggtcgagcg ggtccgctgt ttcctcaaca
     2641 ccgccagcct cggttcctac cccgacctcg tgcgcctgcg cgagcaatgg cagccgcggt
     2701 ggggcaagtg gcctgccttc gctgcagcgc ttgtcgtggt gctgcaccgc gccgaacccg
     2761 tcgagatcaa gatcgacggc cggtggacgt cggtctggtt cctgttcgtc ggcaacgggc
     2821 cctaccaccc gcgcggcatg gtgccggcct ggcgaccctc gctcgactcc ggcctgctcg
     2881 acgtgcgctg gttgcgcgcc gacgtgcggt tctcgcggct gcgggccgtc gtcgcgctgc
     2941 tgctcggcgc actcgggcac agccgcgtgt accaccagcg cgaggtgccc gtgctcgacg
     3001 tcgagctcgc ggtgccgggc atgctcgcca ccgacggtga ggtcgtcgag gaggcgggcc
     3061 ggtacacgtt ccgcgtcgcg aaggacgcga tcccggtgta ccggcggcag gaggacaggt
     3121 ggacggggcg cgaccgcccg ttcgtcggct gagcacgatc accgcaatcg ggacggccct
     3181 cgccctggcc gcctgatcac cagcgtcagg acatctcctg cacaggtgca gctgaggtcc
     3241 cgatcccggc gatcatccgc cgctgccgta gggcttggtc agcacctcga ggttgtgccc
     3301 gtccggcgag gagaagtaca ggccacggcc gccgtcgttc gtgttgtgcc ccggcgtctg
     3361 gtgcccgggg tcgccgtagg tcggcactcc ccgatcgcgc agccgaccgg cgaccgcgtc
     3421 gaactcctcc tcgctgatca agaacgcgta gtgctgcgag ggcacgtcgg cggtgtccat
     3481 gaaatcgagg ctcacgccgt tctgcagctc gacaacggcg aacggtccga acggcaccgg
     3541 gccgggcaac ccgaggacgt cggtgacgaa gcgagcctcc gcgttcttgt cccgcgcggc
     3601 gacgatggtg tggttcagct cgatgctcat ggctaccagc gtgcgacctc aacctgggtg
     3661 caggtcaagg cccggtccgc gataccgccc cgggctacga gccgttggcc gctccgcccg
     3721 gcttgtcgcc ctggtcctcc tggtcgccgt tcacggcgcc gctcgcggcc ggcacgtcat
     3781 caccggccac ggtgggcggg ggcggggcgt cgacggcgcg gttctcctcg ggaaggagat
     3841 cacgcaggac ctggcccgtg atgcgccgga acgtgcgatg cggccgggac cggtcgagca
     3901 cggccacctc cagcgcattg gcgccgagca ccctggtggc ctgcgcgccc ccgttgctcc
     3961 ctgtggggcc ggagccggcc ggggtgccgg tggcctgcaa gccctcgacc gcgataccga
     4021 tcgcctctgc cagctccatt ccgggccggt aggtgtcctt gagcttggcg ctgatcggtt
     4081 cggtggtgcc acccatcacg acgaactgcg gttcgtcagt gatcgagccg tcgtaggtga
     4141 tgcggtagag ctggtcggcc tccggcgtct cgcccaccgc ggccagacac agctcgacct
     4201 cgtagggctt ctgctgctcc acgaaggagg tgccgaggac ctgggcgtag tagttggcaa
     4261 gcatccgccc agtgacgtcg cggcggtcgt aggagtaccc gcgcacgtcg gccatccgga
     4321 tgccggcgtt gcggagcgtc tcgaactcgt tgtagcgccc gaccgcggcg aacccgatgc
     4381 ggtcgtagat ctcggagacc ttgcgcagcg cgctgggccg gttctcgctg gaccggttct
     4441 cggcgacgaa cagcacaccg ccggtgtagg tgagcacgac gacgctgcgg ccgcgggcga
     4501 tgcccttgcg cgccagctcg gagcggtcgc gcagcagctg gtcgacggag gagtagaacg
     4561 gcatcgtcac ggggcggact cctggatcgt cgcaggggtg gggagcacgg tcaggttatc
     4621 cgccgggctt ctccatgcgg ccggccacga cggcacggac gaccgtctcc atctcgtcct
     4681 cgggtcggcg caccgctccc tcgtccgcgg tgatggtgac gaccaccggg tagatccggc
     4741 ggacgagatc cgggccgccg gtggcggtgt cgtcgtcagc ggcgtcgtag agcgcctcga
     4801 cggccgtacg cacggcgagg tcacgatcag ccgcgggatc gtagagcttc ttcagcgccg
     4861 acttcgcgaa caccgagcca gaacccaccg cgtggtagcc gagcagctcg tcgtaccggc
     4921 caccggtggc gtcgtaggtg atgatgcggc ccgccttggc cgggtcggct gcatcgaggt
     4981 cgtacccgac gaacagcggt acgacgacga agccctgcag ggccgcgccg aggttctgct
     5041 ggatcatgct ggcc
//
LOCUS       DASRSC010000166         2462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1692279,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000166 DASRSC010000000
VERSION     DASRSC010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1692279"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VFE39_08075"
     CDS             complement(<1..1006)
                     /locus_tag="VFE39_08075"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HET6258593.1"
                     /translation="MSGTRETAGRSVQRPDFMAKLTGAAQYTVDIGMPGMAHAKILRS
                     DRAHARIVSIDRTAALDAPGVVGVITAADLGGLFPRFGHIVPDHSILAEEKVRYYGEP
                     VAIVVAQSAWAAADALELVRVTYADLPDVLDVAAALAPDAPLVHEHTYGGTADESFRV
                     LTGAEQERASSNLAHEVVLEWGDPDAALAGAATVHTTSVTFPMLYAYAMEPYNAVASY
                     TGDLHVISTAQHPFMVRDDLARVFGLPLSKVRVTSPFVGGGYGSKSYTKVEPLAAIAS
                     WAVGRPVKLVLDVEESIYTTRVDSAEVTMTTGVDSSGRILARVADMVMDSGAYADNSP
                     L"
     gene            complement(1003..1551)
                     /locus_tag="VFE39_08080"
     CDS             complement(1003..1551)
                     /locus_tag="VFE39_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET6258594.1"
                     /translation="MTSTDTAADGVASFPAAGERACTVQTTVNGVRVERALDPRMTLV
                     EFLREDLELTGTKVSCGLQVCGVCTVLVDDQPVSSCTYLAADVDGRAVRTVEGLVREG
                     ALNGLQRSFAEEFALQCGFCTPGFLMMATALLERDPDPTEETIREYLDGNFCRCTGYQ
                     PIVAAVRRAAARKAGEAGGEPS"
     gene            complement(1548..2360)
                     /locus_tag="VFE39_08085"
     CDS             complement(1548..2360)
                     /locus_tag="VFE39_08085"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET6258595.1"
                     /translation="MLPAFALIRPRSLSEALSAISDDDVPYSGGTELLLAMRAGLQRP
                     RALVDLKAVPELRSIALLDGGAEGTELRIGATTTHDELARDAVVRDSFPMLARVAAGV
                     GNARVRAQGTVGGNLCFAEPRSDLVPALAALDASVVLVGPGGSRRVPVAEFILGMYWT
                     VREPDELMLEVRVPVRPVRAHYVNLRITERPSVGVAIRVDADGRCRLVIASVGEQPLV
                     LDYDDMHEVDPDAVSAAVEPVPDLTGAEDYKRHLVAVMIRRAMAELETGKKQ"
BASE COUNT          353 a          888 c          853 g          368 t
ORIGIN      
        1 cgagtgggct gttgtcggcg tacgccccgg agtccatgac catgtcggcg accctggcga
       61 ggatccgccc ggaggagtcg acgcccgtgg tcatcgtgac ctcggcggag tcgacgcggg
      121 tggtgtagat cgactcctcg acgtcgagta ccagcttcac cggccgacct accgcccacg
      181 acgctatggc ggccagcggt tcgacctttg tgtaggactt cgacccgtat cccccgccga
      241 cgaagggcga ggtgacgcgc accttcgaca gcggcaaccc gaagacccgg gcgaggtcgt
      301 cgcggaccat gaacgggtgc tgtgcggtgg agatgacgtg caggtcgccc gtgtaggagg
      361 caaccgcgtt gtagggctcc atcgcgtagg cgtagagcat ggggaacgtg accgaggtgg
      421 tgtgcaccgt cgcggcgccg gccaaggccg cgtccgggtc gccccattcg agcacgacct
      481 cgtgggcgag gttgctgctg gcgcgctcct gctcggcgcc tgtgagcacg cggaacgact
      541 cgtccgccgt cccgccgtag gtgtgctcgt gtaccagcgg cgcgtcgggc gcgagagccg
      601 cggcaacgtc gagcacgtcg ggcaggtcgg cgtaggtgac ccggaccagc tccagggcgt
      661 ccgccgccgc ccacgccgac tgggcgacca cgatcgcgac cggctcgccg tagtaacgga
      721 ccttctcttc ggccaggatg ctgtgatccg ggacgatgtg accgaaccgc gggaagagcc
      781 caccgaggtc ggccgccgtg atgacgccga ccacgcctgg cgcgtccagc gcggccgttc
      841 ggtcgatgct gacgatccgg gcgtgcgcgc ggtcggagcg gaggatcttc gcgtgcgcca
      901 tgcccggcat gccgatgtcc actgtgtact gcgcggctcc ggtgagcttc gccatgaagt
      961 cggggcgctg gaccgaacgg cccgcggtct ccctcgtgcc gctcacgacg gctctccgcc
     1021 ggcctcgccg gccttccgcg ccgccgccct gcgcaccgct gcgacgatcg gctggtaccc
     1081 cgtgcacctg cagaagttgc cgtccaggta ctcccggatg gtctcctcgg tggggtccgg
     1141 gtcccgttcc agcaatgcgg tggccatcat cagaaacccc ggcgtgcaga agccgcactg
     1201 cagcgcgaac tcctcggcaa agctgcgctg cagcccgttg agcgcgccct cgcggaccag
     1261 gccttccacg gtgcgcacgg cccgcccgtc cacgtcggca gccaggtagg tgcaggagct
     1321 gacgggctgg tcgtcgacca gcacggtgca caccccgcac acctgcagcc cgcagctcac
     1381 cttcgtcccg gtcaactcca ggtcctcgcg caggaactcc acgagcgtca tccgcgggtc
     1441 gagggcccgc tccacgcgca ccccgttgac cgtggtctgg acggtgcagg ctcgctcccc
     1501 tgccgccggg aacgatgcca ccccgtcggc ggcggtgtcc gtgctggtca ttgcttcttc
     1561 cccgtctcga gctcggccat cgcccggcgg atcatgaccg cgacgagatg gcgcttgtag
     1621 tcctcggccc cggtgaggtc cgggacgggt tccaccgccg cggagactgc gtccgggtcg
     1681 acctcatgca tgtcgtcgta gtcgagcacc agtggctgct ccccgaccga ggcgatcacg
     1741 agccggcacc ggccgtcggc gtccacacgg atcgccactc ccacgctcgg ccgctcggtg
     1801 atcctcaggt tgacgtagtg ggcgcgaacc ggccggacgg gcacccgcac ctccagcatg
     1861 agctcgtcgg gctcccgcac ggtccagtac atccccagga tgaactcggc gaccgggacc
     1921 cggcggctcc cgcccggccc gaccagcacg accgacgcgt cgagtgccgc gagagccggg
     1981 acgaggtcgg agcgcggctc ggcgaagcac agattgcccc cgaccgtgcc ctgcgcgcgg
     2041 acccgggcgt tgccgacgcc tgcggcgacc ctggccagca tgggaaacga gtcgcgcacc
     2101 accgcatccc gcgcaagctc gtcgtgcgtg gtggtggcgc cgatccggag ctccgtccct
     2161 tccgcgcccc cgtcgagcag cgcgatgctg cgcagctcgg ggaccgcttt caggtcgacc
     2221 agtgcacgcg gtcgctgcaa cccggcccgc atggcgagca ggagttctgt tccgcccgag
     2281 tacgggacgt catcgtcgga gatggctgac agcgcctcgg aaagcgaccg cggccggatg
     2341 agtgcgaagg cggggagcat caggctgcac cgaggctggc gcctgcgttg acgaagaact
     2401 cgtcgaggat ccggttggcc ttcttgtgga tcgtcgggcc gcccatcccg gccacccgcc
     2461 cg
//
LOCUS       DASRSC010000167        18746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3657455,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000167 DASRSC010000000
VERSION     DASRSC010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 18746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18746
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3657455"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            255..545
                     /locus_tag="VFE39_08090"
     CDS             255..545
                     /locus_tag="VFE39_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009151975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HET6258596.1"
                     /translation="MTTTPPPPRTTAAFHVQAVISFAVSLVAAGLAVAYLPADPSVRA
                     FLALAVLYVVTSAFTLAKCVRDRQESAGVISRVDQARLDKLLAEHDPFTVDA"
     gene            complement(552..1076)
                     /locus_tag="VFE39_08095"
     CDS             complement(552..1076)
                     /locus_tag="VFE39_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET6258597.1"
                     /translation="MLGAGLDGMPDQLVGFRGPTACQVVGITYRQLDYWARTGLVVPS
                     IRGAAGSGSQRLYSFKDMLVLKVVKRLLDAGVSLQNIRVAVEHLRRRGIRDLAGITLF
                     SDGTTVYECASAEEVVDLLQGGQGVFGIAVSGAMREISGSIKDFPVERADGGSVDDTP
                     EDELSRRRARRAIS"
     gene            complement(1268..1741)
                     /locus_tag="VFE39_08100"
     CDS             complement(1268..1741)
                     /locus_tag="VFE39_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HET6258598.1"
                     /translation="MSEMRVVGVRVELPANQPILLLRETSGDRYLPIWIGSVEATAIA
                     LEQQGVKPARPLTHDLLKDVITGLGRRLEQVRITDLQEGTFYAELVFDGGIKVSARPS
                     DSVALALRIGVPIHAEESVLAEAGLVIPDEQEDEVEKFREFLDSISPEDFRGAQP"
     gene            complement(1845..2297)
                     /locus_tag="VFE39_08105"
     CDS             complement(1845..2297)
                     /locus_tag="VFE39_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6258599.1"
                     /translation="MTTNDGPGVPPERSPETTSVFRADFLSEAEPPAQDQPVSGVDAL
                     PAGSALLVVKRGPNAGSRFLLDRASTSAGRHPDSDIFLDDVTVSRRHAEFRSDAGEFV
                     VVDVGSLNGTYVNREPVDTAVLANGDEVQIGKFRLVFLTGPREPGAAG"
     gene            complement(2420..2806)
                     /gene="gcvH"
                     /locus_tag="VFE39_08110"
     CDS             complement(2420..2806)
                     /gene="gcvH"
                     /locus_tag="VFE39_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HET6258600.1"
                     /translation="MIPEDLRYTDAHEWVRDMGDGVVRIGITDHAQSQLGDVVFVQLP
                     SVGETVGAGTAVGEVESTKSVSDIYAPVSGTVVAVNESLEASPELINSGPYQAGWMID
                     VRLADDAEGVPGDLLDAAAYRELTGS"
     gene            complement(2816..3442)
                     /locus_tag="VFE39_08115"
     CDS             complement(2816..3442)
                     /locus_tag="VFE39_08115"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET6258601.1"
                     /translation="MPDLPTTQSRADRIVTVPNLLSVLRLAGVPLFLYLLLGPRADGW
                     AILVLAVGGFTDWLDGKLARLLGQYSRLGALLDPAVDRLYILAALVALGVRDVVPWWA
                     VVALVCRDLVLGVTLPVLRRRGYGPYRVTYLGKGATFLLLYAFPLLLLGQGTGWFADL
                     ARPFGYGFAVWGTALYLYSGALYLAQFVLAMRRPALPPGVAGGAGGGD"
     gene            3517..4035
                     /locus_tag="VFE39_08120"
     CDS             3517..4035
                     /locus_tag="VFE39_08120"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HET6258602.1"
                     /translation="MDASALKEIQAPLKARYQQDPGAALITLRADGELDGSGIACRVR
                     TATALAEAGLHPATGGDGTQLCSGDMLLEALVACAGVTLRAVATALGLEVSGTVRAEG
                     DLDFRGTLGVDKEAPVGFRDIRLTFDLDTTADADQVATLTKLTERYCVVLQTLVSSPA
                     TSVRVQTGESRP"
     gene            4338..5231
                     /locus_tag="VFE39_08125"
     CDS             4338..5231
                     /locus_tag="VFE39_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HET6258603.1"
                     /translation="MTRMVEDLRSVDHACAIPVSDEQLWEMTATFVAAGLAAGEQVVY
                     FDDGTSGGLLERLADDCVPVTESLDDGSLTIVPPDTTRATLRSPPPHVQDMICGTIDN
                     AVEQGYTGVRLTGQLSYALRRAGGVSLLQYDAALDEVVRARPARILCLYDRRRFPDGA
                     IEELRARHHTELVVPAIYDDNLLRITSTGPGTARLAGEVDHSNRPRIRRLLETALDQA
                     LRSHAGPTDITLDLSSLRFVDVAGAVSLVHAAEEFPSSHRLVLTGVRPRVLRVLDRCG
                     APFAAQLVVQPRTEQDSGETR"
     gene            5228..6187
                     /locus_tag="VFE39_08130"
     CDS             5228..6187
                     /locus_tag="VFE39_08130"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6258604.1"
                     /translation="MRIETTRSAVAFRLEHAVGYHRSDADLLAQLVPLAAAAIERGEP
                     VAVALRPGTDWALHEELGRPPGLIPLSRPQGSDSASGQTVAARRALELRELTSTTGGP
                     VTLLTEHITTLDGADGSFWTEFDAAVNVALAELPVRMTCFYPELPLHLEILDGSRRNH
                     PLLLVGGQMLHNPEHLYPRDVLAMRPAPAPALLGPPDVRMTFSAWQLHEVRSAVEGSL
                     LEVGYGRTRAEDLVLAVNEVATNAVEHGAPEAELAIWTGNGGELVCEVHDHGVLADPL
                     PGLQAPHPSDPRGRGVWIARQLCDSLHVWADRRGTHVRMHATP"
     gene            6422..7264
                     /locus_tag="VFE39_08135"
     CDS             6422..7264
                     /locus_tag="VFE39_08135"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET6258605.1"
                     /translation="MMQAHSAGPRRVTVLGSFTLTVDGATVPLGVDSRRLVAYLAVHP
                     RPQERPALAADLWPGVPILAAARLLADAAAAVGVPELFDEDGDEAAPCALAADVEVDL
                     ADAMGLVRALPDIPTSHDPDVALLSADILPGWTASWIAVERERFRQLRLHALEERSLR
                     LASAGRYDEAVAMAQTAVQAAPSRESARRALIEAHLAQGNIAAAVAEYDDYQELLRSS
                     VGGPAPSGLEALFPPSPVWPVLRARRPMPRSAVQLPGLRSVRGASGATRRLVAGGSVP
                     GTSR"
     gene            complement(7283..7747)
                     /locus_tag="VFE39_08140"
     CDS             complement(7283..7747)
                     /locus_tag="VFE39_08140"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6258606.1"
                     /translation="MRGHLWSVPNNDDRQERGRLRLRHRASAWELREIRRRVERWAGQ
                     NRLPDDAVIDLQLAVGEAVANGVEHAYRENATDVPTVEVDLELRAQGALQVVAVTVVD
                     HGRWRPVRARPRYRGRGLALIERLSRQFQVSTSGHGTRVCFEIPLHRLQPVG"
     gene            7798..8037
                     /locus_tag="VFE39_08145"
     CDS             7798..8037
                     /locus_tag="VFE39_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258607.1"
                     /translation="MHGSSEPDVAADPHLARTREAGGAEPGREHHPDDERADPDGAST
                     TGTDENEEFVGRATGQDAGYAGETGAEARAEEGSG"
     gene            8034..8348
                     /locus_tag="VFE39_08150"
     CDS             8034..8348
                     /locus_tag="VFE39_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4235 domain-containing protein"
                     /protein_id="HET6258608.1"
                     /translation="MRRDGNELSGLEKIAYKPIGLVFGLIGALVAGKVFGLVWKKLSN
                     EDETPQPLSDDYSTREVLLAATLQGAIFGLIKTAVDRYGMKGARRFLDAPRAPIRART
                     EV"
     gene            8351..8758
                     /locus_tag="VFE39_08155"
     CDS             8351..8758
                     /locus_tag="VFE39_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaB family protein"
                     /protein_id="HET6258609.1"
                     /translation="MPGKDEVPSTVERSDDKAERTWVKTYDSAMDSYDDDGARARRTA
                     FAALKHTHEKVGDHWESKDSYGPSDEQAAHGGVDSDRPTAGGVDANASKKHLLEIAQK
                     LDVRGRSTMTKDELVDAIGKANDRQTAKARKNS"
     gene            complement(8823..9827)
                     /gene="fgd"
                     /locus_tag="VFE39_08160"
     CDS             complement(8823..9827)
                     /gene="fgd"
                     /locus_tag="VFE39_08160"
                     /EC_number="1.1.98.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase
                     (coenzyme-F420)"
                     /protein_id="HET6258610.1"
                     /translation="MALRIGYKASAEQFAPRELVEYAVRAEEVGLDSVVTSDHFLPWR
                     HEGGHAPASLVWLPAVGERTSRVLLGTSVLTPTFRYNPAVLAQTFATLGLLYPGRVML
                     GVGTGEALNEIAVSGREWPEFKERFARLREAVRLMRELWTSEDVTFEGEYYRTVGATI
                     YDKPEQPIPVYVAAGGPVVAKYAGRSAEGFICTSGKGMDLYTEKLLPAVAEGAEAAGR
                     DPGAVDRMIEIKMSYDRDPQKALENTRFWAPLSLTAEQKHSVDSAVEMERLADKLPIE
                     QVAKRWIVASDPDDALAQIKPYVDAGLNHLVFHGPGEDQERFLTQFSEDVLPKLRALP
                     "
     gene            9976..11772
                     /locus_tag="VFE39_08165"
     CDS             9976..11772
                     /locus_tag="VFE39_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6258611.1"
                     /translation="MSESFEQQPHRAALGSLRRLAPYIRPHRRALVGGGLAALAGTLA
                     GLAIPLVTRAIVDGPIARSELSILPWLVAGVFAFGLLEAGLMMLRRRLVARPSTRIEA
                     DMRADLFAHLQRLPVAFHDQWPSGQLLSRATSDLNTIRWFVAFSGIFLVVNVLTMVAG
                     IAVLAWLSPVLGLAIGVIAVPLVVLTLLLERRYSVAARRGQDQAGDLATVIEESALGI
                     RVLKSLGRGRRLTARFVTDARGLRGTELLKVRLLAALWAVLVTLPEAAIALILGLGAY
                     GVAQGTLTLGTLVAAVTVMTYLYWPIASIGWLLAEASNAAAATRRYWEVRDVVPAITD
                     PEAPIAVPRPVRGELVLQDVSFRYPAGHVEVLRGVDLAVRPGETMALVGATGSGKTTL
                     TALVARLYDVTGGAVLIDGIDVRDLRLSDLRTAVATAFEDPVLISASVRENVALGFPS
                     ASDEQVWEALRVASADRFVATLPWGLDTRIGEQGMSLSGGQRQRLALARAVIGHPPVL
                     VLDDPLSALDVHTEAEVEAALRRVLGGVTALVVAHRPSTVQLADRVALLADGRIAAVG
                     THTGLLAADPEYRRLLASMQDDRTDEREEVGA"
     gene            11769..13682
                     /locus_tag="VFE39_08170"
     CDS             11769..13682
                     /locus_tag="VFE39_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6258612.1"
                     /translation="MSTDTGTGTDTDTETDRRSDLATDAAAEAWRGIAAEDDETDAVD
                     RRTGLRLQHRARLLVVELLRPHRGAVVLAVVLLVAETLAMLAGPLLVATAIDSGVPAA
                     LDGRPGPLAWAIAGYAATGVAGAGLRSAFTLLSGRIGQDVLLDLRRRVFAHGQRLSLD
                     FHESYTSGRLISRLTSDMDSLMDLLEKGFEGLFGAVLSLVGVTMLLLWLDPMLAAVVL
                     CGFVPLLVLTRWFQRSSRASYRRTRSTVARLIVHFVESMNGIRAVQAFRRERRNDEIM
                     DRLTTANRDANTTAFLTVAWYVAGVRLVGNATLALMLLIGGLRVLDGTLALGALTAFL
                     LYLRRLYDPLDDLAQFFNSYQSAAAALEKISSVLDTPVGVPEPTRPVALPTRVRGELE
                     FDHVSFAYRRAAERTVLPEFSLRIPAGQTVAVVGATGAGKSTLAKLAARFYDPVAGAV
                     RLDGVDLRDVTDVDLRSALVMVTQEAFLFSGSVADNIAMGRADDLGPPTPAEITAAAE
                     AVGAREFIEALPDGFDTDVRKRGGRLSAGQRQLVSLARVVLADPAVVLLDEATSSLDI
                     PSEQAVQAALETVLAGRTSLIIAHRLSTVLIADRVLVVADGRIVQDGSPEELLAGDGE
                     FTDLHEAWRASLA"
     gene            13699..14013
                     /locus_tag="VFE39_08175"
     CDS             13699..14013
                     /locus_tag="VFE39_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258613.1"
                     /translation="MPPQRPAPEPVSRAAFPFDCHTLARTGLVGAARALWVRDGTRSE
                     RISRALHVAHDVTSTDSNASPAKTPTRSSPGQGIAQLDQRNAEIRAAAVKAEIPADFV
                     RP"
     gene            14217..14828
                     /locus_tag="VFE39_08180"
     CDS             14217..14828
                     /locus_tag="VFE39_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258614.1"
                     /translation="MFALLVGFLAAEVWTSGGRAATAVSDEASSLRAVVLLSSEFPGE
                     TEAQMRSLVRRHIEDAITKEWPQMAEQGARLSAVPQPLADLQAMALGLRPETPGQTIA
                     QREIVTSLEHALDARRQRIIVSQSSVNEAKWGAVVTLAILTLFAIAFVHSGNRAGAAI
                     AMALFASAATAAIIMIAVQEHPFAGNFAVRPTPLEQVEPPLPP"
     gene            14897..15058
                     /locus_tag="VFE39_08185"
     CDS             14897..15058
                     /locus_tag="VFE39_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258615.1"
                     /translation="MSASPNRIIRLPEFVLISRIEGAYIRELQSNDPAVAYNRRPKHQ
                     GPDRMSGPG"
     gene            complement(15317..16519)
                     /locus_tag="VFE39_08190"
     CDS             complement(15317..16519)
                     /locus_tag="VFE39_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6258616.1"
                     /translation="MEPSIGTDPLPGDFFRYADLLTDEEQKVVARVREFLRTEVAPVA
                     LDHWSRAVFPHHLVPGFAALDIAALPYDDLDGPAARRLLIGFIALEIAHTDPSMNSFF
                     GIHNGLAMGSIDLCGSAEQRARWLPAMARMEQIGAFGLTEPHGGSDVARGLETTARRD
                     GSGADAGWVLDGEKRWIGNGTFADLVVIWARDVADDSVKGFVVGQDNPGMTTTKIENK
                     VSLRTVQNADIVLDGCRVLEADRLQNANSFADTNKVLRRTRGGVAWGSTGTMMAAYEI
                     ALEYAKEREQFGRPIAGFQLVQDLLVRMLGNITASLGMAVKLAQMQDAGEARDEHAAL
                     AKEFCTTKMRETVALAREVVGGNGVILDYGVAKFFADAEALYSFEGTKQINTLVVGRA
                     ITGHSAFV"
     gene            16632..17402
                     /locus_tag="VFE39_08195"
     CDS             16632..17402
                     /locus_tag="VFE39_08195"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6258617.1"
                     /translation="MSTELLDRAPTLERRYGRPVHAQIEQWLMDEITHGQIATGDRLP
                     GERALAAALRVSRMTLRQALDGLARRGVLVRLPGRSGGAFVAEPKIDCDLTGLAGFTE
                     QMRRAQRRAGAEVLSARTVGADERVAAALRIPVGAAVHELVRVRSADDAPLALERSWL
                     PAEHLADLLDHPLTGSIYDVLTTHYGLAPHTAVEYLESISADAADAVALAVPRGTALM
                     SVERIAHSVAGLPVEFARDVYRADRVRLMVRTAVSSAM"
     gene            17500..18645
                     /locus_tag="VFE39_08200"
                     /pseudo
     CDS             17500..18645
                     /locus_tag="VFE39_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874037.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
BASE COUNT         2648 a         6753 c         6578 g         2767 t
ORIGIN      
        1 cgtcccggaa cgcgtcctgg atcccctgcg tcgtgatgaa gtccttgttg tagccggcgt
       61 cgttgtagcc ggcgtccccg acaagcgccc agcccggccc ggccaccgtt cctgtgacgt
      121 cccctcggtt gccagggccg acggagggac tccaggatgg cccgtccggg cgggcccggg
      181 aagggccacc ggcgctgggc caacccgtag ttccgctgat gtggccctcg ctgcttgcgc
      241 accagactcc cggcatgacc acgacgcctc ccccgccccg gaccaccgct gctttccacg
      301 tgcaggccgt gatctcgttc gcggtctcgc tcgtcgcggc cggcctggcc gtcgcgtacc
      361 taccggccga cccgtcggtc cgcgcgttcc tcgccctcgc cgtgctctac gtggtgacgt
      421 ccgcgttcac gctggcgaag tgcgtgcgcg accggcagga gagtgcgggt gtcatcagcc
      481 gggtggatca ggcgcgactg gacaaactgc tcgccgaaca cgacccgttc acggtcgatg
      541 catgacctgc gtcagctgat ggcgcgacgg gcgcggcggc gggacagctc gtcctccggc
      601 gtgtcgtcga cgctgccgcc gtcggcacgc tccaccggga agtccttgat cgagccgctg
      661 atctcccgca tcgcaccgct gacggcgatg ccgaagacgc cctgcccgcc ctgcaacagg
      721 tcgacgacct cctcggcaga cgcgcactcg tagacggtgg tgccgtcgct gaacagcgtg
      781 ataccggcga gatcgcggat cccacgccgg cgcaggtgct cgaccgccac tcggatgttc
      841 tgtagcgaca cccctgcgtc cagcagccgc ttcacgacct tgaggaccag catgtccttg
      901 aacgagtaca gccgctgcga acccgaaccc gccgctccac ggatggacgg cacgacgagc
      961 cccgtgcggg cccagtagtc gagctgccgg taggtgatcc ccacgacctg gcaggccgtg
     1021 gggccccgga agccgacaag ctgatcgggc atcccgtcga gcccggctcc cagcaaggga
     1081 tcggggaaga gctcgccctg ttcgggtgag tcgaacggcg ggttctccac cgaactcctt
     1141 ccgccggggc ccgaaaagcg ggccgtcgac ccgacggtaa gcgggtgcgc gcggcgggtc
     1201 aacgcggcgc gccgccgcgg gtcacccagc accgggcgtg tcgagacctt gaacccgttt
     1261 gcgagcccta cggctgggcc ccgcggaagt cctccggcga gatcgagtcc aggaattcgc
     1321 ggaacttctc gacctcgtcc tcctgctcgt ccgggatgac caggcctgcc tccgcgagca
     1381 cgctctcctc cgcgtggatc ggcactccga tccgcagcgc gagtgcgacc gagtcgctcg
     1441 gtcgggccga caccttgatg ccgccgtcga agacgagctc ggcgtagaac gtgccttcct
     1501 ggagatcggt gatccgcacc tgctccaggc gacggcccaa gcccgtgatc acgtccttga
     1561 ggaggtcgtg ggtcagcggt cgggcgggtt tgaccccctg ctgctccaga gcgatcgcag
     1621 tggcctcgac cgaaccgatc cagatcggga ggtaccggtc accactggtc tcccgcagca
     1681 ggaggatcgg ctggttcgcg ggcagttcga cccggacgcc cacgacgcgc atctcgctca
     1741 tgcgcttccc tcctcctgac ggtcctgctg tgtcgacgct acacggcaca accggcccgc
     1801 gcccgcctcc ggacgggcgg cgaaccgtcg tcctgcgcct ggtctcagcc tgctgcacca
     1861 ggctcgcgcg ggccggtgag gaagaccagc cggaacttgc cgatctgcac ctcgtcacca
     1921 ttggccagca cggcggtgtc gaccggctcc cggttgacgt aggtgccgtt gaggctgccc
     1981 acgtcgacga cgacgaactc gcctgcgtcg ctgcggaact ccgcatgccg ccgcgagacg
     2041 gtgacgtcgt cgaggaagat gtcgctgtcc gggtggcggc ccgcgctggt ggatgcgcgg
     2101 tcgaggagga accgcgatcc cgcgttgggt ccccgcttga cgaccagcag ggcggagcct
     2161 gccggcagcg cgtcgacgcc ggagaccggc tggtcctggg cggggggctc ggcctcggag
     2221 aggaagtccg cacggaagac cgaggtggtc tccggggagc gctccggggg cacgccaggc
     2281 ccgtcgttcg tggtcacctc ggggtctcct cctgcatgga tgtagcggcg tcagtgtggt
     2341 ggtacggatc tggaagccgc acgtgctgtg ccgtgggttc cgcaaaccta ccccgcggag
     2401 ccgcccagct cagggccggt cagcttccgg tcagctcgcg gtatgccgcg gcgtcgagca
     2461 ggtcgcccgg cacgccctcg gcatcgtcgg ccagccggac gtcgatcatc cagccggcct
     2521 ggtaggggcc cgaattgatc agctcgggag aggcctcgag ggactcgttc accgcgacca
     2581 cggtgccgga caccggggca tagatgtcgg ataccgattt ggtcgactcc acctcgccaa
     2641 cggccgtgcc ggcgccgacg gtctcgccga ccgagggcag ctgcacgaag acgacgtcgc
     2701 cgagctggga ctgggcgtgg tcggtgatgc cgatcctgac caccccgtcc cccatgtcgc
     2761 gcacccattc gtgggcatcg gtgtagcgga ggtcctcggg gatcacgcaa cgagactagt
     2821 ccccgccccc ggccccgcct gcgacccccg gcggcagtgc gggtcggcgc atcgccagca
     2881 cgaactgcgc caggtaaagg gcgcccgagt agagatagag cgcggtgccc cagaccgcga
     2941 agccgtatcc gaaggggcgc gccaggtcgg cgaaccatcc ggtcccctgg ccgagcagga
     3001 gcaacgggaa cgcatagagc agcaggaacg tggcgccctt gccgaggtag gtgacccggt
     3061 agggcccgta acccctgcgg cgcaacaccg gcagggtcac gcccagcacc aggtcgcggc
     3121 acacgagcgc caccacggcc caccacggca cgacgtcccg cacgccgagc gcgaccagcg
     3181 cagcgaggat gtagagccgg tccaccgccg gatcgagcag ggcaccgagg cggctgtact
     3241 gcccgagcag ccgggccagc ttgccgtcga gccagtcggt gaaccccccg acagcgagca
     3301 cgaggatcgc ccacccgtcg gcccgcgggc cgaggagcag gtagagaaac agcggcaccc
     3361 cggccagccg caggacgctg agcaggttcg ggaccgtcac gatgcggtcg gcccgggact
     3421 gcgtggtggg caggtcgggc accccgggag cctactggtg gatccccgtc cacctcacat
     3481 gtacgcagcg ggaccggcag aggcaggatc atcggcatgg atgcatctgc gctgaaggag
     3541 atccaggccc ccctcaaggc ccgctaccag caggaccccg gcgccgcgtt gatcacgttg
     3601 cgggcggacg gcgaactcga cgggagcggc atcgcctgtc gcgtccgcac cgcgacggcg
     3661 ctggccgagg ccggtctgca tccggcgacc ggcggcgacg gcacgcagct gtgctcaggc
     3721 gacatgttgc tcgaggcgct cgtcgcgtgc gccggcgtca cgctccgcgc cgtcgccacc
     3781 gcgctcgggc tcgaggtgag cggcacggtc cgggccgagg gcgacctcga cttccgcggc
     3841 acgctcggcg tcgacaagga ggcgccggtc gggttccgcg acatccgcct gaccttcgac
     3901 ctcgacacca ccgccgacgc cgaccaggtc gcgaccctca ccaagctgac cgagcggtac
     3961 tgcgtggtgt tgcagacact ggtgtcctcg ccggcgacgt ccgtccgggt gcagaccggg
     4021 gagagcaggc cctgaggaac ggctgaactc cgaggcccgg tcgaggtcgc cggccgggcc
     4081 ccgaacgtcg cctgcgagcg gcgggatcgg ctcggccggt gtcccgacga gtgatgtccg
     4141 tcgcccggtc ggtggacagc ttctcctcct gggcccgttc gtcgcggctg atcgccgctt
     4201 cgccgcgcgg ccccgtcccg gagtgctccc gacccctggt gggtggacgt acagtgtcct
     4261 ttgcgtgacg cagcgttctg cccgttctgc cgggccgccg cgtcccggac gatgtcgggc
     4321 tccgcaggga ttgcgtgatg acgagaatgg tcgaggacct ccggtctgtc gaccacgcct
     4381 gcgccattcc tgtgagcgac gagcagctgt gggagatgac ggccacgttc gtcgccgccg
     4441 ggctggcggc aggcgagcag gtcgtgtact tcgacgacgg cacctccggg ggcctcctcg
     4501 agcggctggc ggacgactgc gtcccggtca ccgagtcgct cgacgacggc agcctcacca
     4561 tcgtgccgcc cgacaccacg cgcgcgacgc tgcgcagccc gccgccacac gtgcaggaca
     4621 tgatctgcgg gaccatcgac aacgcggtcg agcaggggta cacgggcgtc cgcctgaccg
     4681 gacagttgag ctatgcgctg cggcgggcgg gcggggtgtc cctgctccag tacgacgccg
     4741 cgctggacga ggtggtccgg gcccgcccgg ccaggatcct ctgcctctac gatcggcgcc
     4801 gtttccccga cggcgccatc gaggagctgc gcgcccggca ccacaccgag ctcgtcgtgc
     4861 cggcgatcta cgacgacaac ctgctgcgga tcacgagcac cgggcccggc accgcgcggc
     4921 tggccggaga ggtggaccac agcaaccggc cgcggatccg tcggctgctg gagacggcgc
     4981 tggaccaggc gctgcggtcg cacgcggggc ccaccgacat cacgctcgac ctctcctccc
     5041 tgcggttcgt cgatgtcgcg ggcgccgtca gcctcgtgca tgcggcggag gagttcccga
     5101 gctcgcaccg gctggtgctc accggtgtcc gaccccgcgt gctgcgcgtg ctcgaccgct
     5161 gcggtgcgcc gttcgcggca cagctcgtcg tccagccgcg gaccgagcag gattccgggg
     5221 agacgcgatg aggatcgaga ccacccggtc ggccgttgcc ttccgcctgg agcatgcggt
     5281 ggggtaccac cggtccgacg ccgacctgct cgcccagctg gtaccgctgg ccgcggcggc
     5341 catcgagcgc ggggagccgg tcgcggtcgc gctgcggccc ggcacggact gggcgctcca
     5401 cgaggagctg gggaggccac cggggctgat cccgctgagc cgcccacagg gctcggacag
     5461 cgcgtccggg cagacggtgg ccgcgcgcag ggcgttggag ctgcgcgagc tgacgtccac
     5521 caccggcggc ccggtcaccc tgctgaccga gcacatcacc accctcgacg gcgccgacgg
     5581 cagcttctgg accgagttcg acgcggcggt gaacgtcgcg ctggcggagc ttcccgtgcg
     5641 catgacgtgc ttctacccgg agctgccgct gcacctcgag atcctggacg ggtcgcggcg
     5701 caaccacccg ttgctgctgg tgggcggcca gatgctgcac aaccctgagc acctctaccc
     5761 ccgggacgta ctcgcgatgc gccccgcgcc cgctcccgcg ctgctgggcc cgcccgacgt
     5821 ccggatgacg ttcagtgcgt ggcagttgca tgaggtgcgt tcggccgtcg aaggctccct
     5881 tctcgaggtc ggctacgggc ggacccgtgc agaggacctc gtcctcgcgg tgaacgaggt
     5941 ggccaccaac gccgtcgagc acggcgctcc cgaggcggag ctggcgatct ggaccggcaa
     6001 cggcggcgag ctggtgtgcg aggtccacga ccacggtgtc ctcgccgatc ccctccccgg
     6061 gctgcaggcg ccccacccct cggacccccg gggacgtggt gtgtggatcg cccggcagct
     6121 gtgcgactct ctccacgtgt gggccgaccg ccggggcacc cacgtccgaa tgcacgcgac
     6181 accgtgagta agatcggatg tgcgtgtcac ccggacggcc gtccagcggc cggaagtgtg
     6241 acgaatatgc cccttggcaa cgcgcccggt cgggagaaca ctccttcgcg gactcgtgcg
     6301 ccatcgggcg gccgacaggg cacacgcgca gtaccacttt cgagcaccac tataagcaga
     6361 accacattcg ggcaccatca catttgggac caccatgtct gacgacgagc gacgatctcg
     6421 catgatgcag gctcactcag ccggtccgcg ccgggtcacc gtgctcggct cgttcacttt
     6481 gaccgtcgac ggcgctaccg tgccgctcgg ggtcgactcc cgccggctcg ttgcgtacct
     6541 cgcggtgcat ccacggcccc aggagcgccc ggcgctggcc gcggacctgt ggcccggcgt
     6601 ccccatcctc gcggccgcgc gcctgctcgc cgacgctgcc gcagcggtcg gcgtgccgga
     6661 gctgttcgac gaggacggcg acgaggccgc gccgtgcgcg ctggccgccg acgtcgaggt
     6721 cgacctcgcc gacgcgatgg gtctcgtccg cgcgctgccc gacatcccga ccagccacga
     6781 tccggacgtc gcgctgctct cggccgacat ccttcctggc tggacggcct cgtggatcgc
     6841 cgtcgagcgc gagcggttcc gacagctgcg actgcacgct ctcgaggagc gcagcctgcg
     6901 gctggcgtcc gcgggccggt acgacgaggc cgttgccatg gcgcagaccg ccgtgcaggc
     6961 ggctccgagc cgcgagagcg cccgccgcgc cctcatcgag gcacacctgg cccagggcaa
     7021 catcgcggcg gccgtcgccg agtacgacga ctaccaggag ctgctgcggt ccagtgtcgg
     7081 cggccccgct ccgtccgggc tggaggcgct gttcccgccc tcgccggtat ggccggtact
     7141 ccgggcgcga cgccccatgc cgcggtcggc cgttcagctg ccgggcctgc gctcggtacg
     7201 cggggcgtcc ggtgccaccc ggcggctcgt cgccggaggt tcggtccccg gcacgtcacg
     7261 ctgaccaagg cacgtccggc gtctacccga ccggctggag ccggtgcagg gggatctcga
     7321 agcacacccg ggtgccgtgg cccgaggtgg acacctggaa ctgccgcgac agccgctcga
     7381 tgagggccag gccgcgacct cggtagcgcg gccgggcccg caccggtcgc cagcgcccgt
     7441 ggtcgaccac cgtcaccgcc acgacctgca gggctccttg cgcccggagc tccaggtcca
     7501 cctcaacggt ggggacatcc gtggcgttct cgcggtaggc gtgctccacg ccgttcgcca
     7561 ccgcctcccc gacggccagc tgcaggtcga tcacggcgtc gtcggggagc ctgttctgcc
     7621 ctgcccagcg ctccactcgc cggcgaatct cgcgcagctc ccaggcggat gcgcgatggc
     7681 gcaaccgcag ccgacctctc tcctgccggt cgtcgttgtt cggcacgctc cacaggtgcc
     7741 cccgcacccg agagcgcaaa ccgtgtcaga cgggcactga tcgggtactc gccgctcatg
     7801 catggttcct ccgagcccga tgtcgccgct gacccgcacc tcgcacgcac ccgggaggcc
     7861 ggcggcgcgg aacccggccg cgagcaccac cccgacgacg agcgggccga cccggacggc
     7921 gccagcacca ccggcaccga cgagaacgag gagttcgtcg gccgggcgac gggccaggac
     7981 gccgggtatg ccggtgagac cggcgccgag gcacgcgcgg aggagggttc cggatgaggc
     8041 gcgacggcaa cgagctgagt ggactcgaga agatcgccta caaacccatc ggcttggtct
     8101 tcgggctcat cggggccctg gtggccggca aggtattcgg cctggtgtgg aagaagctgt
     8161 cgaacgagga cgagacgccg cagccgctgt ccgacgacta cagcacccgc gaggtgctcc
     8221 tcgccgccac gctgcagggc gcgatcttcg gcctcatcaa gaccgcggtg gaccggtacg
     8281 ggatgaaggg cgcgcggcgc ttcctggacg cgccgcgggc accgatccgc gctcgaacgg
     8341 aggtgtaggg atgccgggca aggacgaggt tccgagcacc gtggagcgct cggacgacaa
     8401 ggccgagcgg acctgggtga agacgtacga ctcggccatg gacagctacg acgatgatgg
     8461 cgcccgagcc aggcgcacgg cgttcgcggc gctgaaacac acccacgaga aggttggcga
     8521 ccactgggag tccaaggact cctacgggcc gtccgacgag caggccgccc atggcggtgt
     8581 ggacagtgac cggcccacgg cgggcggcgt cgacgcgaac gcctcgaaga agcaccttct
     8641 cgagatcgcg cagaagctcg acgtgcgcgg ccggtcgacg atgaccaagg acgagctggt
     8701 cgacgcgatc gggaaggcca acgaccgcca gacggcgaag gcccgcaaga acagctgacc
     8761 cgacccggga ccgccccgct cagccgagcc gggcggtgcg ttccgcggcg gaaaagcttc
     8821 ggtcagggca gcgcgcgcag cttcggcagg acatcctcgg agaactgcgt gaggaaacgc
     8881 tcctggtcct cccccggccc gtgaaagacg aggtggttga gcccggcgtc gacgtagggc
     8941 ttgatctggg ccagggcgtc gtccgggtcc gacgccacga tccagcgctt ggccacctgc
     9001 tcgatcggca gcttgtcggc cagccgctcc atctcgaccg ccgagtcgac gctgtgtttc
     9061 tgctccgcgg tgagtgacag cggcgcccag aaccgggtgt tctccagcgc cttctgcggg
     9121 tcgcggtcgt aggacatctt gatctcgatc atccggtcga ccgcgccggg gtcacgcccg
     9181 gccgcctcgg cgccctcggc cacggccggc agcagcttct ccgtgtagag gtccatgccc
     9241 ttgccggagg tgcagatgaa gccctcggcc gaccggcccg cgtacttcgc gacgaccggg
     9301 ccgcctgccg ccacgtacac cgggatgggc tgttccggct tgtcgtagat cgtggccccg
     9361 acggtgcggt agtactcgcc ctcgaacgtc acgtcctcgg acgtccacag ctcgcgcatg
     9421 aggcggacgg cctcacgcag ccgcgcgaag cgctccttga actccggcca ctcccggccc
     9481 gacacggcga tctcgttgag cgcctcaccg gtgccgacgc ccagcatcac gcggcccggg
     9541 tagagcaggc ccagcgtcgc gaaggtctgc gccagcaccg cggggttgta acggaacgtc
     9601 ggcgtgagca ccgacgtccc gagcagcacc cgcgacgtgc gctcccccac cgcaggaagc
     9661 cacacgagcg aggcgggagc gtgcccgccc tcgtgccgcc acggcaggaa atggtccgac
     9721 gtcaccacgc tgtcgagtcc cacctcctcc gcccgcaccg cgtactccac gagctcccga
     9781 ggcgcgaact gctccgccga tgccttgtag ccgatccgca acgccacgtc ctcagatcct
     9841 ttcccgatcc ggtggttctg ctccctccag agtgctacac gcgggtgctt gacctccact
     9901 cgcgtcgagg tcgcacggtg tggccatgag aagtcgcatg cgtccgacgt cggaagtacc
     9961 taccgtgatc gggccatgag cgaatcattc gagcagcagc cgcaccgtgc ggccctcggt
    10021 tcgctgcgcc gcctcgcccc ctacatccgc ccccaccgcc gggcgctggt cggaggcggc
    10081 ctcgccgcgc tcgccggcac ccttgccggg ctggcgatcc cactcgtcac ccgtgccatc
    10141 gtcgacggcc cgatcgcccg gtccgaactg tcgatactgc cgtggcttgt tgccggggtg
    10201 ttcgccttcg ggctgctcga ggcaggcctg atgatgctgc gccggcggct cgtcgcccgc
    10261 ccgagcaccc gcatcgaggc agacatgcgg gccgacctgt tcgcgcacct gcagcggctg
    10321 cccgtcgcct tccacgacca gtggccgtcg gggcagctcc tctcgcgcgc gacgtcggac
    10381 ctcaacacga tccgctggtt cgtcgcgttc tcggggatct tcctcgtggt caacgtgctg
    10441 accatggtgg cgggcatcgc ggtgctggcg tggctctccc ccgtgctggg tctggccatc
    10501 ggggtcatcg cggtgccgct cgttgtgctc accctcctgt tggagcgccg ctactcggtc
    10561 gccgcgcgcc gtgggcagga ccaggcgggg gatctcgcca cggtgatcga ggagtcggcc
    10621 ctcggcatcc gcgtgctcaa gtcgctcggc cggggacgca ggctcactgc ccggttcgtc
    10681 accgacgcgc gcgggctgcg gggcaccgaa ctgctgaagg tgcggctcct cgctgccctg
    10741 tgggccgtgc tcgtgacgct gcccgaggcg gcgatcgcgc tgatcctcgg gctgggggct
    10801 tacggggtcg cccagggcac gctgacgctc ggcaccctcg tggccgcggt caccgtcatg
    10861 acgtacctgt actggccgat cgcctcgatc ggctggctgc tggccgaggc cagcaacgcc
    10921 gcggcggcca cgcggcggta ctgggaggtt cgtgacgtcg tcccggcgat caccgacccg
    10981 gaagccccga tcgcggtccc gcgcccggtt cggggcgagc tcgtcctgca ggacgtgtcg
    11041 ttccggtacc cggccgggca cgtggaggtc ctgcgcgggg tcgacctggc agtgcgcccc
    11101 ggggagacga tggcgttggt cggcgccacg gggtcgggca agaccacgct cactgcgttg
    11161 gtggcccggc tctacgacgt caccgggggc gcggtgctga tcgacggcat cgacgtgcgc
    11221 gacctgcggc tgtccgacct gcgcaccgca gtggccaccg cgttcgagga cccggtgctg
    11281 atctcggcca gcgtgcggga gaacgtcgcg ctcgggttcc cgtccgcgtc ggacgagcag
    11341 gtgtgggaag ccctgcgggt ggccagcgcc gaccgtttcg tcgccacgct gccgtggggc
    11401 ctcgacacca ggatcgggga gcagggcatg tcgctctcgg gcgggcagcg ccagcggctc
    11461 gcgctcgcca gggcggtgat cgggcacccg cctgtcctcg tgctggatga cccgctctcc
    11521 gcgctggacg tgcacaccga ggccgaggtc gaggcggcgc tgcggcgggt gctgggcggg
    11581 gtgaccgccc tggtcgtggc gcaccggccg tccacggtcc agctcgccga ccgggtcgcg
    11641 ctgctggccg acggccggat cgcggccgtc ggcacgcaca ccgggctgct ggccgccgac
    11701 ccggagtacc gccggctgct ggcgagcatg caggacgacc ggaccgacga gcgagaggag
    11761 gtgggcgcgt gagcacggac accggcacgg gcaccgacac ggacacggag acggacagga
    11821 ggtcggacct ggcgaccgac gctgcggccg aggcctggcg cggtatcgcg gctgaggacg
    11881 acgagaccga cgccgtggac cggcgaaccg ggctgcggct gcagcaccgc gcccgcctgc
    11941 tcgtcgtgga gctgctgcgt ccgcaccggg gcgccgttgt gctggcggtg gtgctgctcg
    12001 tcgcggagac cctggcgatg ctcgccgggc cgctgctggt ggccaccgcg atcgacagcg
    12061 gggtgcccgc tgccctcgac gggcggcccg gcccgctggc ctgggccatc gcggggtacg
    12121 ccgccaccgg ggtcgcgggg gccggcctgc ggtcggcgtt cacgctgctg tccggccgga
    12181 tcgggcagga cgttctgctc gacctgcgcc gccgggtgtt cgcccacggg cagcggctgt
    12241 cgctcgactt ccacgagtcc tacacgtccg gccgcctgat ctcgcgcctg accagcgaca
    12301 tggactcgct catggacctg ctggagaagg ggttcgaggg cctgttcggc gccgtcctct
    12361 cgctggtcgg cgtcacgatg ctgctgctgt ggctcgaccc gatgctcgcc gcggtcgtgc
    12421 tgtgcggctt cgtgccgctg ctcgtgctca cccgctggtt ccagcgcagc tcgcgggcga
    12481 gctaccggcg cactcgcagc acggtcgcgc ggctgatcgt gcatttcgtc gagtcgatga
    12541 acggcattcg cgccgtgcag gccttccgcc gcgagcggcg caacgacgaa atcatggacc
    12601 ggctcaccac ggccaaccgc gacgccaaca cgaccgcgtt cctcaccgtg gcctggtatg
    12661 tggcgggggt gcgcctcgtc ggcaacgcga cgctggcgct catgctgttg atcggtggtc
    12721 tgcgggtgct tgacggcacg ctggcactgg gcgccctcac cgcgttcctg ctgtacctgc
    12781 gccggctcta cgacccgctc gacgatctgg cgcagttctt caacagctac cagtcggccg
    12841 ccgccgcgct ggaaaagatc tcctccgtgc tcgacacgcc ggtcggcgtg cccgagccga
    12901 cccgtccggt ggcgctgccg acccgcgtgc ggggcgagct ggagttcgac cacgtcagtt
    12961 tcgcctaccg gagggcagcc gagcgcacgg tgctcccgga gttctcgctg cgcatcccgg
    13021 ccgggcagac ggtcgccgtc gtcggggcca cgggtgccgg caagtcgacg ctggccaagc
    13081 tcgcggcgcg gttctacgac ccggtcgcgg gcgcggtccg ccttgatggg gtggacctac
    13141 gcgacgtcac cgacgtcgac ctgcgctccg cgctggtgat ggtcacgcag gaggcgttcc
    13201 tgttctccgg ctcggtcgcg gacaacatcg cgatgggccg ggcggacgat ctcgggccgc
    13261 ccacccccgc cgagatcacg gctgccgccg aggcggtcgg ggcgcgtgag ttcatcgagg
    13321 cgctgcccga cgggttcgac accgacgtgc gcaagcgcgg cggcaggctc tcggcggggc
    13381 agcgccagct cgtgtcgctc gcgcgggtgg tgctggccga ccccgcagtg gtgctgctcg
    13441 acgaggccac gtcgtcgctg gacatcccgt cggagcaggc ggtgcaggcc gcgctggaga
    13501 ccgtgctcgc cggccgcacg tcgctgatca tcgcccaccg gctctcgacg gtgctgatcg
    13561 ccgatcgcgt gctcgtcgtg gctgacgggc ggatcgtgca ggacgggtcg ccggaggagc
    13621 tgctggccgg cgacggcgag ttcaccgacc tgcacgaggc gtggcgcgcg tccctcgcct
    13681 gagcgggacg acgggtccat gcccccgcaa cgccccgcac ccgagccggt gtcgcgcgcc
    13741 gcgttcccct tcgactgcca caccctggcc cgcaccggcc tcgtcggcgc cgcccgtgcc
    13801 ctgtgggtac gcgacggcac ccggagcgag cgcatcagcc gcgcactgca cgtggcccac
    13861 gacgtgacga gtaccgacag caacgcgtcg cccgcgaaga cacccacaag gtcttccccc
    13921 ggtcagggga tagcccaact cgaccagcgc aacgccgaga tccgagcggc cgcagtcaag
    13981 gcggaaatcc cggcggattt cgtgcgacct tgaattcgac gccggcggcg aggactatcc
    14041 acagtgtgcc ggactggctg cacgaccttc ccgcgccctg gttggccgtg ttcgtgttcg
    14101 cgattgccgt tctcgtcacg gcggcgatct acgcgacggt gatctcgctc tcgaccggtg
    14161 agcgcgggcg cgcgttcgcg tcaatctcac cggggctgct cccgccgatg ggaatcatgt
    14221 tcgcgctgct ggtgggtttt ctggccgcgg aggtctggac ttcaggaggc cgcgctgcga
    14281 ccgcggtcag cgacgaggcg agctcgctgc gcgcggtcgt cctgctttcc tccgagttcc
    14341 ccggtgaaac ggaggcacaa atgcggagcc tcgttcgccg tcacatcgag gacgcgatca
    14401 cgaaagagtg gccacagatg gccgagcagg gagcgaggct gtccgccgtg ccgcagccgc
    14461 ttgcagatct ccaagcgatg gcgctcggcc tgcgccctga aactcccggc cagacaatcg
    14521 cccagcggga gatcgtcaca tcgctcgagc atgcgctgga cgcccggcgc cagcgcatca
    14581 tcgtgagcca gtcctccgtc aacgaggcca agtggggagc cgtggtcacc ctggcgatcc
    14641 tcaccctgtt cgcgatcgcg ttcgtccaca gcggcaaccg agccggagcc gcgatcgcga
    14701 tggcgctctt cgcctcggcg gcgaccgcgg cgatcatcat gatcgcggtc caggagcacc
    14761 cgttcgccgg gaacttcgct gttcgtccga cgccactcga gcaggtcgag cccccgctgc
    14821 cgccctagcc gagctgacac ggtcctttct gccgaatgaa tctcgtcgcc caccggttcg
    14881 ctgtcctgcg tggtgattgt cggcgagccc gaaccggatc atccggctcc ccgagttcgt
    14941 gctgatctcc cggatcgagg gcgcctacat ccgcgagctg cagtccaacg atcccgccgt
    15001 cgcctacaac cggcggccca agcaccaggg ccccgaccgc atgtccggac cgggctgatc
    15061 gtggaaccag cggtcggcga ctccaccacg tcgcgccgct ctaccgcggc gatcgcctcc
    15121 agggcccccg ccaggtcgac ggccggcctc ctgcctggcc tcgtggctgg cgcgagcagg
    15181 tcgaccgcgc ccggcgagga ggggggactc ggacgccgtg gcacggccga cagtgatgaa
    15241 gcgcgatgat ctcgtgatgc gttacacaac gtaacgattg ggagttgata ccggctagag
    15301 ctcagaggac gcgctgtcat acgaaggcgc tgtgccctgt gatggcgcgc ccgacgacga
    15361 gggtgttgat ctgcttcgtg ccctcgaagg agtagagcgc ctcggcatca gcgaagaact
    15421 tcgcgacccc gtagtcgagg atcaccccgt tgccgccgac cacctcgcgg gcgagcgcga
    15481 cggtctcccg catcttcgtg gtgcagaact ccttggccag cgcggcgtgc tcgtcccggg
    15541 cttcccccgc gtcctgcatc tgcgcgagct tcacggccat gccgagtgac gcggtgatgt
    15601 tgccgagcat gcggaccagc aggtcctgca cgagctggaa cccggcgatg ggccggccga
    15661 actgctcgcg ctccttcgcg tactccagcg cgatctcgta ggcggccatc atcgtgccgg
    15721 tggagcccca tgccactccc ccgcgcgtgc ggcgcaacac cttgttcgtg tcggcgaagg
    15781 agttggcgtt ctgcagccgg tccgcctcaa ggacgcggca gccgtcgagc acgatgtcgg
    15841 cgttctgcac ggtccgcagc gacaccttgt tctcgatctt ggtcgtggtc atgcctgggt
    15901 tgtcctggcc gacgacgaaa cccttgaccg agtcgtcggc gacatcgcgc gcccagatca
    15961 cgacgaggtc ggcgaaggtg ccgttgccga tccagcgctt ctcgccgtcg agcacccatc
    16021 ccgcgtccgc tcctgagccg tcgcgccgag cggtggtctc gagcccgcgt gccacgtccg
    16081 agccgccatg cggctcggtg aggccgaatg caccgatctg ctccatgcgt gccatcgcgg
    16141 gcagccagcg cgcgcgctgc tccgctgagc cgcagaggtc gatgctgccc atcgcgaggc
    16201 cgttgtggat gccgaagaag ctgttcatcg acgggtcggt gtgggcgatc tccagcgcga
    16261 tgaacccgat cagcaggcgg cgcgcggcgg gcccgtccag gtcgtcgtag ggcagggcgg
    16321 cgatgtcgag ggccgcgaag cccggcacca ggtggtgcgg gaacacggca cgggaccagt
    16381 ggtcgagggc caccggcgcc acctcggtgc gcaggaactc ccggaccctg gccaccacct
    16441 tctgctcctc gtccgtgagc aggtcggcat agcggaagaa gtcgccgggg agtgggtcgg
    16501 taccgatcga gggttccata cccgacgatc gcaccgcgct cccggcgcgg caaccggatg
    16561 acggtcccat gcatcgaggc cgccggtcgg ggagttcgcc gtgtgaacgc gagcccgcct
    16621 accctgagca cgtgtcgacg gagctgctcg atcgcgcccc caccctggag cgtcgctacg
    16681 gacgtccggt gcacgcgcag atcgagcagt ggctcatgga cgagatcacg cacgggcaga
    16741 tcgccaccgg cgaccgtctg cccggcgagc gggcactggc ggcggcgctg cgggtgagcc
    16801 gcatgacgct gcggcaggcg ctcgacggcc tcgcccgccg cggagtgctc gtccggttgc
    16861 ccgggcgctc gggtggagcg ttcgtggccg agccgaagat cgactgcgac ctgacggggc
    16921 tggccgggtt caccgagcag atgcgccggg cgcagcggcg ggccggggcc gaggtgctgt
    16981 cggcgcgtac ggtgggcgcc gacgagcggg tagcggccgc gctgcgcatc ccggtgggtg
    17041 cggcggtcca cgagctcgtg cgggttcgct cggcggacga cgcaccgctc gccctggagc
    17101 ggtcctggct gcccgcggag cacctcgccg acctgctcga ccacccgctc accggatcga
    17161 tctacgacgt gctcacgacc cactacggtc tcgccccgca cacggctgtc gagtacctgg
    17221 agtcgatcag cgccgacgcg gccgacgcgg ttgcgctcgc ggtgccccgg ggcacggcgc
    17281 tgatgagcgt cgagcgcatc gcgcattcgg tggccgggtt gccggtggag ttcgcccgcg
    17341 acgtctaccg tgccgaccgg gtgcggttga tggtgcgcac ggcggtgagc agcgcgatgt
    17401 aggaatcgcc tgtgcgcggc gccactgcgc gacccgtacc tcgcgtcgtt ctcgcgaaga
    17461 ggacaggacg tgtcctcttc ggctcaaacc gtcgcctgca tgaatgacac ggagatccgc
    17521 tctttccgca ttgacgtccc gcaggctgac ctcgacgacc tggtgcggcg gctcgggcac
    17581 acccgctaac ccgacgagga acctggcgcc ggctggaaca acggcatccc gctggggtac
    17641 tcgaaggagc tggccgagta ctggcgcagc agctacgact ggcgcctcca tgaagcccgg
    17701 ctcaacgcgc tcccgcagtt ccggagcatg atcgacggcc acgatgtgca tttcctgcac
    17761 gtccgctccg cccagccgga tgcgctgccg atcgtcctga cccacggctg gccggggtca
    17821 atcgtcgagt ttctcgacgt catcggaccg ctgaccgatc cggtcgcgca cggcggggac
    17881 gcccgggacg ccttccacgt ggtcgtcccc accatccccg gtttcgggat ctccggcccg
    17941 gcggtgggct ggtcgacgtc gcgcgtggca gtggcgtggg ccgagctgat gcgcaggctc
    18001 ggctactccc gctacaccgc acacggtggc gacaccgggg cgatgatctc ccgcgagctc
    18061 ggtctcgtcg acccggggca cctcgtcgcg ctgcacctga cctatatccc atcggcgaag
    18121 gtgagcgccg agacggccga cttctccgac ctcgcggagc agcggtccgt cgagaacctc
    18181 taccgcttcc agcacgagct cggcgcctac gcgatgttgc aggccacgcg cccccagacg
    18241 ctgtcgtacg cgctcaccga ctcccccgtc gggcagttgg cgtggatcgt cgagcggttc
    18301 aaggactgga ccgactcgaa ggacacaccc gaggacgctg tcgaccgcga cgcgatgctg
    18361 accaacgtga tgatctactg gctgaacggc accgccggct cgtcggcccg ctactacaag
    18421 agcgggaacg agggctgggg cgaacccgaa ccgccgtccc ccgtgcccac cgcgatcgcg
    18481 gtcctgccct atgacatcgg ctccccggcg cggcgtgtcg cggagcagaa ccacgagatc
    18541 gtgcgctgga cggagtacga gcggggcggg cacttcgccg cgatggagga gccggacctg
    18601 gtggtggcgg atctgcgcga gtccttccgg ccctaccgtc gctgagggcg ccggggaccg
    18661 catccggccg gagcagccgg gccgtcccgg tccggggctc ccgtcgggga cgttccggcc
    18721 cgggccgacg gcggcggcgc ctacga
//
LOCUS       DASRSC010000168        17254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4969161,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000168 DASRSC010000000
VERSION     DASRSC010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 17254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17254
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4969161"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VFE39_08205"
     CDS             <1..442
                     /locus_tag="VFE39_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6258618.1"
                     /translation="PLIALGPLLITTAGREATPTLVAALAAGFAVFVAATSALGSASE
                     AQRDVFTVLGAGRASTLLRLQLPAGLPLIVDGLTLAAPAAVLGAVIGEWFGARRGIGV
                     LLVSSMQNVQTDQLWAAALTAAAISLLAYVLLVALRGAVNRRFQ"
     gene            513..1352
                     /locus_tag="VFE39_08210"
     CDS             513..1352
                     /locus_tag="VFE39_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6258619.1"
                     /translation="MRAISIDALPASRRVPVAHEGGARPSPARLLARGLGRVLSRYWT
                     VAAFVVAWQLWSWLTGYNQIVVPPPAMVFGDVVGNIGAYLPDLGWTLAMSAAGLVAGM
                     ALGTMIAVVVWSSRIVSGMMTPVALVMRSVPVVAMIPVLARVFGYSWFTVLVMTTVVS
                     FFPAFVLVGSRLRAAPQATSDLMAVLGASRSTVLRLLLLPHAVPGLLVAFRLTAPTAV
                     LAAMLAEYLMGGRGLGTLFAQSVSFHEVGRAWGAALVATVVSVACFVAARAVERRGTA
                     RFS"
     gene            1403..2482
                     /locus_tag="VFE39_08215"
     CDS             1403..2482
                     /locus_tag="VFE39_08215"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6258620.1"
                     /translation="MSSEPAPWSRRRLLRTAGIAGLGLGLAPAVLAACGAGGDTGGGD
                     TATTKLRVALGWITNVEFAGFWLADDRGYYKQEGLDVEFLPGGPNAPDPTQTLAARGA
                     EIAIPVAMQAFLSAVNQGNDFVAYGTMFQNAPSALLSLTKKPVRTAQDLVGARVLGQQ
                     GVQPYVEAAMRLAGVPVQYEFIPVGYEPSPLVEGQGDVYTCFATNQPITLEQQGMVQD
                     RDFVVTTYEQLGLPQYSSPLCSKRSWLDANRPIVEKFMRATIRGWQDNAADPSVAAHL
                     AVEKYGADLGLDLAQQTRENELQIPYTQSELTTSSGLFRIDPARLSGPMYAAYTAAGV
                     TPLPDVAKIVDTGVLDNVFQGKATV"
     gene            2482..3468
                     /gene="add"
                     /locus_tag="VFE39_08220"
     CDS             2482..3468
                     /gene="add"
                     /locus_tag="VFE39_08220"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HET6258621.1"
                     /translation="MVRDLARLPKAHLHLHLEGAMRPSTLTDLAAAAGVAVPPVRGYA
                     SFSEFGLQYRSASALIATEADLRRVVREVIDDAADDGALWVEPHFYPPRYTGQLGTAD
                     EVLEIVMDEGQRAAEARGIGCGWIVSALRDFDPAQAVELAKLAARYAGSGVVSFGLAA
                     DEALFGPEPFAEAFGIARDAGLISAPHAGELAGPASVYGALDALGAQRICHGVRAIED
                     PALVERLATDGIVLDVCPTSNVMLAVVPSFDEHPLVRLLEAGVRCTINGDDPLLFGPG
                     LLAEYELVRNTMGLSDERLAQLARSSLVGSGAPVELVSDGVQLIEEWLTTAT"
     gene            complement(3544..4401)
                     /locus_tag="VFE39_08225"
     CDS             complement(3544..4401)
                     /locus_tag="VFE39_08225"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HET6258622.1"
                     /translation="MPALDLTAFADLGVFTPAAGSDAAKAFYDRWGFMVLRDLVDPAL
                     IDAMEAECVAAQQGVLAGELDARYGSRKYLDDESKAERFVNYVEHIEELAPALRTALA
                     TRALVELVTQILGPDFWPGRGGVVYQDARPGRESGYTRIGWHADWQSVPHLDIWPGTA
                     FTFHIDGTSPANGFLRVVPGSHLWATASPFENINNVSVPEGAPAARGYTDTPPPAPMP
                     LGFEKIRGEIPVYAERGDVILHDAYLWHSAARATDDDTVRRHVRGGWHGGDTSLMDRE
                     TGFVKNAAR"
     gene            complement(4492..5055)
                     /locus_tag="VFE39_08230"
     CDS             complement(4492..5055)
                     /locus_tag="VFE39_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6258623.1"
                     /translation="MNEPGARDRILAAAEELFADCGFDATPTSRIAERAGVPKGLVHY
                     YFKRKPDLLTALVERLPEDRVDPAHVVVPGDVAASLHRLVDVLDRTLDSSAMLSHLLW
                     READTHPAVLDALRSRFGRLVEQIRAVVAAAQPRCAAADVDSAAHLLASAVSYRHSVA
                     RHAEDGQDTMHRELRFLADALAAGAGA"
     gene            complement(5156..5518)
                     /locus_tag="VFE39_08235"
     CDS             complement(5156..5518)
                     /locus_tag="VFE39_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPW repeat protein"
                     /protein_id="HET6258624.1"
                     /translation="MTRRSWHRWQDWTALVIGVLTALSPIVVSTNATALWTLIVFGVL
                     IAATALWSLAQPDSVASEYVHAVLGVLLFISPWVLAYSDLGGASWTSWVAGVLTVAVG
                     LAALPSANAEHRGLAGQH"
     gene            complement(5630..6256)
                     /locus_tag="VFE39_08240"
     CDS             complement(5630..6256)
                     /locus_tag="VFE39_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HET6258625.1"
                     /translation="MAHFDLAWQPSLLDATADGVELDPGFPEVRRRELGEGAWVDHGP
                     GWCRGADDLCARLLDTTPWAGREVRMYDRVVPEPRLTHRWRLDDGPGPPAELCEMARV
                     LSERYGVAFTQVGVNLYRDGADSVAWHGDRVARDLPEAIVALVSLGAVRPFRLRPTGG
                     GASIAYLPGPGDLLVMGGSCQRTWQHSVPKTRSVGPRISIQFRHAYQR"
     gene            6343..7119
                     /locus_tag="VFE39_08245"
     CDS             6343..7119
                     /locus_tag="VFE39_08245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HET6258626.1"
                     /translation="MIDHRSAYGLVTDFDPFRRIEADTLALADAAEGHLTAAVPSCPG
                     WSVADLVWHLLEVQHFWGEIVTGRVQDPATVPEAVRPAEARLIPELRAGVARLVTTLR
                     GAEPAEPVYSWAARRDAGFVVRHQVQEAAVHRWDAEHAIGRESSLDAAAATDAVEEFL
                     EISTPFRADGAAAVGGRLELVAVDTGIRWVAGEDGHGTARWERLAAGAHPAAQEADAV
                     LRASASDLLLYLYRRRSAGALEVTGDVPVAERFALRTGTD"
     gene            complement(7175..8749)
                     /locus_tag="VFE39_08250"
     CDS             complement(7175..8749)
                     /locus_tag="VFE39_08250"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HET6258627.1"
                     /translation="MPAASRPRRSRVVAAATVALGALVASLLGATPAAAASGGGAASP
                     TCDEHAGSSVDDLLECVTVEGVRAHLDALQDIADANDGTRASGSPGYDASAEYVADEA
                     RRAGLAVTTQDFEFPFFQLNSQTLARTAPTPATYTAGTDFAAMTFSGSGDVSGTAMPV
                     DLRLPSTGGSTSGCEAADFAGFPAGGIALLQRGTCDFGIKARNAEAAGAAGALIFNEG
                     DAPDRTELLQGTLGAPIGIPVMGAAFAIGEALQNATVRLQVDAISENRTTTNVFAETP
                     HGGDTVLMVGAHLDSVPEGAGINDNGSGSASILEVARQVAGAETDATVRFAWWGAEEL
                     GLLGSEHYVATLPQPERDRIAGYLNFDMVGSPNFVRFVYDGDDSDAVGAGPGPAGSAA
                     IEDVFEKFYADRGLAQEGTDFSGRSDYGPFIAAGIPAGGLFTGAEGVKTPEQAAVYGG
                     TAGAAFDACYHQACDTIANINDTVLGENADAMASAILTLASDPALITGPATPAGPAGA
                     PAGGGGLAHEHGALRS"
     gene            complement(8882..10315)
                     /locus_tag="VFE39_08255"
     CDS             complement(8882..10315)
                     /locus_tag="VFE39_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6258628.1"
                     /translation="MDTAARISSEHGLPGHLVDFVAALRRHGVSVGPGETIDAARVLG
                     ALDLIHREHVREGLAAALLRRSGQRQAFDTLFELYFPAALGAGSGEVEVPRVDDAEGG
                     PVDVDALRDILADLLRDGDTAALAELARAVVDALGRSGAAGTGIQGAGGRPSWSAYQA
                     LRTLSPETLLARILADLQDATGDDTAFSDEVLRREIRDRIAAFRAMITDEVRRRTAEI
                     RGREEVAKSAVPKQIEQVEFLSAYAEQLAQLRRTVHPLARRLASRLAVRRKHAKRGSL
                     DMRRTLRRSMSTGGVPMRPAYRTRRPGRPELVILCDVSGSVAGFSHFTLLLVQALREQ
                     FSKVRVFAFVERADEVTHLFDPGAELSGVMQRVLREADLVAFDGHSDYGGSFGSFAEH
                     WGDAVTSKSSLLVLGDARTNYRDPNLAVVKRLVAQARHAHWLNPEPRRQWGSGDSAAL
                     RYADVLPMHECRTAAQLTAVVEALLPV"
     gene            complement(10337..11221)
                     /locus_tag="VFE39_08260"
     CDS             complement(10337..11221)
                     /locus_tag="VFE39_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019510639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6258629.1"
                     /translation="MGRVSAPQSPLFGSVADVADRLSGVGYLASTAVATTVFLADRLG
                     KPLLVEGPAGVGKTELAKAVAAATGSGLVRLQCYEGIDEARALYEWNHAKQLLRITAG
                     QGKESWDETRDDVFSEEFLLPRPLLTAIRRNEPTVLLVDEMDKADVEVEGLLLEVLSD
                     FQVTVPEMGTIIATRRPFALLTSNSTRELSEALKRRCLFLHLDFPDADLERRIVLSRV
                     PELAESLVDALVRTVRVLRGLDLRKSPSVAETIDWGRTLLALGLDTLDDEAMRATLGV
                     VLKHQSDAVKAAAELRLN"
     gene            11408..13510
                     /locus_tag="VFE39_08265"
     CDS             11408..13510
                     /locus_tag="VFE39_08265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HET6258630.1"
                     /translation="MTTGIPTQQDLRRRAEDVLATLAGPAARLREDQWNAIEALVAQR
                     RRALVVQRTGWGKSAVYFVATALLRAAGAGPTVIVSPLLALMRNQIDAAQRAGIHAVT
                     VNSANTSDWERTYAAVDAGEVDVLLVSPERLNNPDFRDRVLPRLTASAGMLVVDEAHC
                     VSDWGHDFRPDYRRLRALIAELPAGIPVLATTATANDRVVTDVAEQLGLGSHDAGDTL
                     VLRGSLDRESLRLSVVRLPTPAQRLGWLAAQLDALPGAGIIYTLTVSAAEEVAEFLRE
                     RGHPVASYTGKTDPEERLAAEGDLLANRVKALVATSALGMGFDKPDLGFVVHLGAPAS
                     PVAYYQQIGRAGRAVERAEVVLLPGREDRDIWAYFASLAFPPEPLVRQTLRVLRPEPL
                     STAAIETRVDLSRTRLEMLLKVLDSDGAAKRVKGGWVTTDEPWVYDEERHRRIAEARR
                     AEQQAMLDYLDTAECRLVFLRRQLDDLDAQPCGRCDNCAGPAWSTDVDAGAATAAEER
                     LTRPGVEVPPRKQWPSGMAELGVPVSGRIAAAELAEVGRVIGRLSDIGWGTRLRELVG
                     GTDVPVPADVLDACVRVLAGWGWTERPVGVVGIGSRTRPHQLDHLARRIAEIGRLPLL
                     GTLVPAGPRPGEHSNSAQRLAAVWGAFEAPTFAPTGGPLLLVDDLVDSGWTITVATRL
                     LRQAGASAVLPFALAVAG"
     gene            complement(13529..14770)
                     /locus_tag="VFE39_08270"
     CDS             complement(13529..14770)
                     /locus_tag="VFE39_08270"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6258631.1"
                     /translation="MAAADRARARMVDVLCDGDRIRSPAVEDAFRAVPRHVFLPGLAL
                     DEAYADEAVAVQFSDGVATSSASQPSMMAIMLEQLDLRPGHHVLEIGAGTGYNAALMA
                     RIVGPAGAVTAVDIDEDLIARAALHLAEAGVSGVELVAADGALGHPAGAPYDRVVLTV
                     GSSDIRPEWVAQLGPGGRLLLPLAVRGSQLSTAFDLGPEGMLHSDSVRSCAFIRLRGV
                     GASSDAGTRLDGVGLVLQVAEDGPGADPAGVTAALAEPGALLPVPAALGTADVWDGFG
                     LWLALTEPGLCRLLVCAPETGLSDDLLPLGNGAGTIALATTAGERPPGLAVVVAGSGA
                     DVRPGSVAVRVFGAAGPELARRLLASLDRWTAAGRPGAAEWRIAAMPAGAVGEAVPPG
                     VSVVRKEHCCLLVDLPGVPAN"
     gene            complement(14984..16108)
                     /gene="proB"
                     /locus_tag="VFE39_08275"
     CDS             complement(14984..16108)
                     /gene="proB"
                     /locus_tag="VFE39_08275"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET6258632.1"
                     /translation="MTARRQLAAARRIVVKVGSSSLTSLDGGLDPLRLDALVDALATR
                     RATGGQVVLVSSGAIAAGLAPLRLARRPRDLATQQAAASVGQLLLAQAYAASFGRHGQ
                     AIGQVLLTADDMIRRAHYRNAQRTLERLLGLDVLPVVNENDTVATDEIRVGDNDRLAA
                     LVAHLIGADGLVLLSDVDGLYDGDPRLPGSRLLGEVDAPSDLESVRVARPGAGSLGRG
                     GMATKITAAAMAAAAGIPVLLAAAEDVTSALDASEVGPKVGTAFRPSGRRMSARRFWL
                     LHAADVRGSLDLDAGAVAAVMDRRRSLLAAGIHGISGDFVAGDVVDLVGPHGVVVARG
                     VVGFDATELPALIGRRSRDLAPEQRREVVHADDLVPLTTP"
     gene            complement(16105..>17254)
                     /gene="obgE"
                     /locus_tag="VFE39_08280"
     CDS             complement(16105..>17254)
                     /gene="obgE"
                     /locus_tag="VFE39_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET6258633.1"
                     /translation="PTRKAPGFALLGEPGDTLDLVLELRSMADVGLLGFPSAGKSSLV
                     SALSAARPKVADYPFTTLVPQLGVVTAGEDVFTVADVPGLIPGASEGRGLGLDFLRHI
                     ERCSVLVHVVDCATFEPGRDPVSDVQALEDELARYTPALGGELSDRPRLIALNKIDVP
                     DAQDMAELVTAELTERFGWPVFPISAASRAGLRELSFAMAEQVRAYRAAQPILEPTRI
                     VLRPQAVDDAGFTVEADRELPGGFIVRGARPERWIRQTSFENDEAVGYLADRLARLGV
                     EEALAQAGAEAGCPVTIGDVTFDWEPSTPAGVAVLMSGRGTDRRLERDDRIRAAERKA
                     ARGERRKHRTDEELLADESSDDPAGPRWDDATWDDEPVESGERPRTRP"
BASE COUNT         2404 a         6293 c         6200 g         2357 t
ORIGIN      
        1 gccgctgatc gcgctgggcc cgctgctgat caccacggcc ggacgcgagg ccactccgac
       61 cctggtcgcc gcgctggccg cgggctttgc cgtcttcgtc gccgcgacgt cagcgctggg
      121 atcggcgagc gaggcccagc gggacgtgtt cacggtgctc ggcgcgggac gagccagcac
      181 cctgctgcgg ctgcagttgc ctgccggact cccgctgatc gtcgacggtc tcaccctcgc
      241 cgcgcccgcc gcggtgctcg gcgccgtcat cggggagtgg ttcggcgcgc gccgcggcat
      301 cggcgtcctg ctggtcagtt ccatgcagaa cgtccagacc gaccagctgt gggccgcggc
      361 gctgacagcg gccgcgatct ccctgctcgc ctacgtgctc ctggtggcgc tgcgcggagc
      421 cgtgaaccgg aggttccagt gagcgagctt gcgagcgaat cagtgtcaca gcgcctacgc
      481 gcagcgccga ccgagcgcag cgagggagtg cgatgagagc catcagcatc gacgcgctgc
      541 ccgcgtcccg ccgcgtgccg gtcgcacatg agggcggcgc ccggccctcg cctgcgcggc
      601 tgctcgcgcg aggcctcggg cgcgtcctct cccggtactg gacggtggcc gcgttcgtgg
      661 tcgcgtggca gctctggtcc tggctcaccg gctacaacca gatcgtcgtt cccccgcctg
      721 ccatggtgtt cggtgacgtc gtcggcaaca tcggcgccta ccttcccgac ctcggatgga
      781 cgctcgccat gtcggcggcc gggctggtcg ccgggatggc actcggcacc atgatcgcgg
      841 tcgtggtgtg gtcgtcgcgg atcgtctccg gaatgatgac gccggtcgcg ctcgtcatgc
      901 gatcggtgcc ggtggtcgcg atgatcccgg tgctcgccag ggtgttcggc tacagctggt
      961 tcacggtcct ggtgatgacc accgtggtct cgttcttccc ggcgttcgtg ctcgtcgggt
     1021 cccggctccg agccgcaccg caggccacgt ccgacctcat ggcagtactg ggcgcctccc
     1081 gctccaccgt gctgcgcctg ctgctgctcc cgcacgccgt gcccggacta ctggtggcct
     1141 tccggctcac cgcgcccacg gccgtgctcg cggcgatgct cgcggagtac ctgatgggcg
     1201 ggcgtggcct cggcacgctc ttcgcacagt cggtgagctt ccacgaggtc ggccgggcct
     1261 ggggagccgc actggtcgcg acggtcgtct ccgtcgcctg cttcgtcgcc gcccgcgccg
     1321 tcgagcgccg gggcaccgcc cggttcagct gagccctgcc ccacccattc ccgctccccc
     1381 cgatccaccc cgaggagacc gcatgtccag cgagcccgcc ccctggtccc gccgccggtt
     1441 gctgcgcacg gcaggcattg ccggcctcgg cctcggcctc gcgcctgccg tcctggcagc
     1501 ctgcggagca ggcggcgaca ccggtggcgg cgacaccgcc accacaaagc tgcgcgtggc
     1561 gctgggctgg atcacgaacg tcgagttcgc cggcttctgg ctcgccgacg accgcggcta
     1621 ctacaagcag gagggcctcg atgtcgagtt cctccccggc ggtccgaacg ccccggaccc
     1681 gacgcagacc ctcgccgcac gcggcgccga gatcgccatc cccgtggcaa tgcaggcgtt
     1741 cctatcggcc gttaaccagg gcaacgactt cgtcgcctac gggacgatgt tccagaacgc
     1801 gccgtcggcg ctgctgtcgc tgaccaagaa gccggttcgc accgcccagg acctcgtcgg
     1861 cgcccgggtg ctcgggcagc agggtgtgca gccctacgtc gaggcggcga tgcggctggc
     1921 gggcgtgccc gtccagtacg agttcatccc ggtcggctac gagccgagtc cgctggtcga
     1981 gggccagggc gacgtctaca cctgcttcgc caccaaccag ccgatcaccc tcgagcagca
     2041 gggcatggtg caggaccgcg acttcgtcgt cacgacctac gagcagctcg ggctgccgca
     2101 gtactccagc ccgctgtgca gcaagcgctc ctggctggac gcgaaccgcc ccatcgtcga
     2161 gaagttcatg cgcgcgacga tccgcggctg gcaggacaac gccgccgacc cgtcggtcgc
     2221 cgcgcatctc gcggtggaga agtacggcgc cgacctcggc ctcgacctcg cccagcagac
     2281 ccgggagaac gaactgcaga ttccgtacac acagagcgag ctgacaacgt ccagcgggct
     2341 gttccggatc gacccggcgc gcctgtccgg ccccatgtat gcggcgtaca cggccgccgg
     2401 ggtgaccccg ctccctgatg tcgcgaagat cgtcgacacc ggcgtgctcg acaacgtctt
     2461 ccagggaaag gccaccgtct gatggttcgc gacctcgccc gactgccgaa ggcccatcta
     2521 cacctccacc tggagggagc gatgcggccc tcgacgctga ccgacctggc ggccgccgcg
     2581 ggggtggcgg tcccgccggt gcgtggctac gcctcgttca gtgagttcgg gctgcagtac
     2641 cggagcgcgt ccgcgctgat cgccaccgaa gccgacctgc gccgggtggt gcgcgaggta
     2701 atcgacgacg ccgcggatga cggcgcgctg tgggtggaac cgcatttcta cccgccgcgc
     2761 tacaccggac agctcggcac cgccgacgag gtgttggaga tcgtcatgga cgaggggcag
     2821 cgcgcagctg aggcccgggg gatcggctgc ggctggatcg tctcggcgtt gcgcgacttc
     2881 gaccccgccc aggcggtgga gctggcgaag ctggccgccc gctatgcggg ttccggagtg
     2941 gtgtcgttcg ggctggcggc ggacgaggcg ctgttcgggc cggagccgtt cgcggaggcg
     3001 ttcgggatcg cccgcgacgc cggactgatc tccgcgccgc acgcgggcga gctggcgggg
     3061 ccggccagcg tctacggcgc gctcgacgcg ttgggcgcgc agcgcatctg ccacggcgtt
     3121 cgcgcgatcg aggacccggc gctggtggag cggctcgcca ccgacgggat cgtgctcgac
     3181 gtgtgcccga cgtccaacgt gatgctcgcc gtcgtgccgt cgttcgacga gcacccgctg
     3241 gtccggctgc tggaggcggg cgtgcgctgc accatcaacg gcgacgaccc tctgctcttc
     3301 ggccccgggc tgctggcgga gtacgagctg gtccgcaaca cgatgggcct gtccgacgag
     3361 cggctggcgc agctcgcgcg ctcgtcgctg gtgggctccg gcgcaccggt ggagctcgtg
     3421 tccgacggcg tgcagctcat cgaggagtgg ctgaccaccg cgacctgaca ggggcccggc
     3481 ctccagcggg gcgacctgct cgcgtccgac gtgagcaggg cgcccgctga gaatccggcc
     3541 gggtcagcgc gccgcgttct tcacgaagcc cgtctccctg tccatcagtg acgtgtcgcc
     3601 gccgtgccag ccgccgcgga cgtggcgccg cacggtgtcg tcgtcggtgg cgcgtgctgc
     3661 cgagtgccac aggtacgcgt cgtgcaggat cacgtcgcca cgctcggcgt agaccgggat
     3721 ctcaccgcgg atcttctcga agccgagcgg catgggtgcg ggcggcgggg tgtcggtgta
     3781 gccgcgagcg gcgggggcgc cctcggggac gctcacattg ttgatgttct cgaacggcga
     3841 cgcggtggcc cagaggtggc tgccgggtac gacgcggagg aagccgttgg ccgggctggt
     3901 gccgtcgatg tggaacgtga acgcggtacc cggccagatg tcgaggtgcg gaacggactg
     3961 ccagtccgcg tgccagccga tgcgggtgta gccggactcg cggccgggcc gggcgtcctg
     4021 gtagacgaca ccgccgcgcc ctggccagaa gtcgggcccg aggatctgcg tcaccagctc
     4081 gacgagcgcc cgcgtggcaa gtgcggtacg gagcgcaggc gcgagctcct cgatgtgctc
     4141 cacgtagttg acgaaccgct cggccttgct ctcgtcgtcg aggtacttcc gcgagccgta
     4201 gcgggcgtcc agctcccccg cgagcacccc ctgctgggcg gccacgcact ccgcctccat
     4261 ggcgtcgatg agcgctgggt ccaccaggtc gcgcaggacc atgaagcccc accggtcgta
     4321 gaacgccttg gcggcgtcgg agccggctgc gggggtgaac acgccgaggt cggcgaaggc
     4381 ggtcaggtcg agagcgggca cgtcgactcc tgtgtgtgaa ccggacggtt cacacagtgt
     4441 gaaggacgcc tgccccgcgc ggcgttacgg tccggtgaaa acgacggagg gctacgcgcc
     4501 ggcgcctgcg gccagcgcgt cggccaggaa acgcagctcg cgatgcatgg tgtcctggcc
     4561 gtcctcggcg tgccgggcca ctgagtgccg gtagctcacg gccgaggcca gcaggtgcgc
     4621 ggcgctgtcg acgtcggcag cggcgcagcg cggctgcgcg gccgcgacca cggcgcggat
     4681 ctgctcgacc agccggccga aacggcttcg cagcgcgtcg agcacggcgg gatgggtgtc
     4741 agcctcccgc cagagcaggt gcgagagcat cgccgacgag tcgagggtgc ggtcgaggac
     4801 atcgacgagc cggtgcaggc ttgctgcgac gtcgccgggc acgaccacgt gcgccgggtc
     4861 gacgcgatcc tccggcagcc gctcgaccag cgccgtcaac aggtccggct tgcgcttgaa
     4921 gtagtagtgc acgaggccct tcggcacgcc tgcgcgctcc gcgatccgtg aggtcggcgt
     4981 ggcgtcgaac ccgcagtcgg cgaacagctc ctccgcagcg gccaggatcc ggtcgcgcgc
     5041 cccaggctcg ttcatgtccc actcacccgt ccatcctgac gcccaaccat cctgaagcgc
     5101 agcacggccg cggccaacag gccacggccg tgctgtcgat cgtttccgcg tcgactcagt
     5161 gctgcccggc cagcccccgg tgctccgcat tcgcggacgg cagggcggcg aggcccaccg
     5221 cgaccgtcag cactccggcg acccaggacg tccaggacgc cccaccgagg tcgctgtagg
     5281 cgagcaccca cggcgagatg aacaacagaa cgcccagcac tgcgtgcacg tactcactcg
     5341 ccaccgaatc cggctgcgcc agcgaccaga gtgcagtggc ggcgatgagc acgccgaaca
     5401 cgatgagcgt ccacaacgcg gtggcgttgg tgctcaccac gatcggcgac agggccgtca
     5461 gcactccgat caccagcgcc gtccagtcct gccacctgtg ccatgacctg cgggtcatcg
     5521 cgatcaccct tccctcgtga ggagacgatc agatgacttc atggtgcgcc tgactggccg
     5581 cccggtcaag ttcgtccgtc ctgttcgtct gcgatctgga ccacaggagt cagcgctggt
     5641 aggcgtggcg gaactggatg ctgatgcgcg gcccgacgga gcgcgtcttg ggcaccgagt
     5701 gctgccacgt gcgctgacag gaaccgccca tgacgaggag gtcgcccggt ccggggaggt
     5761 acgcgatcga cgcgccgccg ccggtgggac gcaaccggaa cggccggact gcacccagcg
     5821 acaccagtgc gacgatcgcc tccggaaggt cgcgggcgac ccggtcgccg tgccaggcca
     5881 cactgtcggc gccgtcgcgg tagagattga cgcccacctg cgtgaatgcc acgccgtacc
     5941 gctcgctcag cactcgcgcc atctcacaca gctccgcggg cggacccgga ccgtcgtcga
     6001 gacgccagcg gtgggtgagc cgcggctccg gcacgacccg gtcgtacatc cgcacctctc
     6061 ggccggccca cggggtggtg tcgaggagcc gggcgcagag gtcgtcggcg ccgcgacacc
     6121 aacctggccc gtggtcgacc cacgccccct cccccagctc gcggcgccgc acctcgggga
     6181 aaccgggatc gagctcgacg ccgtccgcgg tggcgtcgag cagcgacggc tgccaggcga
     6241 ggtcgaaatg ggccacctcc gcgacggtac cccatatcga acatcagttc gaggttgtcc
     6301 gtcggcaacg ttctgtcggc ccctccggct agcgtcgccc tcgtgatcga tcatcgcagc
     6361 gcctacgggc tcgtcaccga cttcgatccc ttccgccgga tcgaggccga cacgctcgcg
     6421 ctcgccgacg ccgccgaagg ccacctcacc gcggccgtgc cgagctgccc cggatggtcg
     6481 gtggccgacc tggtgtggca cctgctcgag gtgcagcact tctggggcga gatcgtgacc
     6541 ggccgcgtac aggacccggc gacggtgccc gaggcggtgc ggcctgccga ggcccggctg
     6601 atccccgaac tgcgcgcggg cgtcgcccgg ctcgtcacga cgctgcgcgg ggcggagccc
     6661 gccgagccgg tctacagctg ggcagcccgg cgcgacgccg ggttcgtggt gcgacaccag
     6721 gtgcaggaag ccgccgtcca ccggtgggac gccgaacacg ccatcgggcg ggagagctct
     6781 ctcgacgccg cggcggccac cgacgcggtg gaggagttcc tggagatcag cactccgttc
     6841 cgggccgacg gggcagcagc ggtcggcggc aggctcgagc tggtcgccgt cgacaccggc
     6901 atccgctggg tcgccgggga ggacgggcac ggcaccgcac gctgggagcg gctggccgcc
     6961 ggagcgcacc ccgccgcgca ggaggcggac gccgtgctgc gggcatccgc ctccgacctg
     7021 ctgctctacc tctaccgccg ccggtccgca ggcgccctgg aggtcaccgg cgacgtgcct
     7081 gtcgccgagc ggttcgccct ccgcaccggc accgactgac ggcgcaggct cccgcccgca
     7141 ggcggagatc cccgcggcgc cgaccgcacc atcgtcagga gcggagggcc ccgtgttcgt
     7201 gggccagccc gccaccgcct gccggtgcgc ccgccggccc agccggtgtc gctgggcccg
     7261 tgatcagtgc cggatcggac gccagggtga gtatcgcgga ggccatcgcg tcggcgttct
     7321 cacccaggac cgtgtcgttg atgttggcga tggtgtcgca ggcctggtgg taacaggcgt
     7381 cgaacgcggc cccggccgtt ccgccgtaca cggccgcctg ctcgggagtc ttgacgccct
     7441 ccgcgccggt gaacaacccg ccggctggga tgcccgcagc gatgaacgga ccgtagtccg
     7501 agcgcccgct gaagtcggtg ccctcctggg ccaggccgcg gtcggcgtag aacttctcga
     7561 acacgtcctc gatggccgcc gatcccgcag ggccggggcc cgcgcccacg gcgtcggagt
     7621 cgtcgccgtc atagacgaag cggacgaagt tgggcgagcc caccatgtcg aagttcaggt
     7681 agcccgcgat ccggtcgcgc tcgggctgcg gcagcgtcgc cacgtagtgc tccgacccga
     7741 gcagcccgag ctcctcggcc ccccaccagg cgaaccgaac cgtggcatcc gtctccgccc
     7801 cggccacctg ccgcgcgacc tcgaggatcg aggcgctgcc gctgccgttg tcgttgatgc
     7861 cggcgccctc gggcaccgag tcgaggtgcg ccccgaccat cagcaccgtg tcgccaccgt
     7921 gcggcgtctc ggcgaagaca ttggtggtgg tgcggttctc gctgatcgcg tcgacctgca
     7981 gccgcacggt ggcgttctgc agcgcctcgc cgatggcgaa cgcggcgccc atcaccggga
     8041 tgccgatggg cgcgccgagc gtgccctgca gcagctcggt gcggtccggc gcgtcgccct
     8101 cgttgaagat caacgcgccc gccgcgcccg cggcctcggc gttgcgcgcc ttgatgccga
     8161 agtcgcaggt gccgcgctgc aagagggcga tgccaccggc ggggaagccg gcgaagtctg
     8221 cggcctcaca accactggtg gagccgcccg tcgagggcag ccggaggtcg accggcatag
     8281 ccgtgccgct cacgtcgccg ctgccggaga aggtcatggc cgcgaagtcg gtgcctgcgg
     8341 tgtaggtggc gggcgtcggt gcggttcggg cgagtgtctg gctgttcagc tggaagaacg
     8401 ggaactcgaa gtcctgagtg gtgacggcca ggccggcccg gcgggcctcg tcggccacgt
     8461 actccgccga ggcgtcgtaa cccggcgagc ccgatgcgcg cgtgccgtcg ttggcgtcgg
     8521 cgatgtcctg cagggcatcc aggtgcgcgc ggaccccctc gaccgtgacg cactccagca
     8581 ggtcgtcgac cgagctgccc gcgtgctcgt cgcacgtcgg gctcgccgca cctccacccg
     8641 aggccgccgc ggcaggggtg gctcccagca gtgaggccac gagtgcgccg agtgcgactg
     8701 tggctgcggc gacgacccgt gatcgacggg gtcgcgaggc tgcgggcatg atcgctccac
     8761 ctttgtcgcg gtaaaccctt ttgtccgggt tacgtcaatg gaggagaatg ccgatctctg
     8821 tcacgcaggg tcaagatcta gattcaatcg atacacatgt gctagatggc gggttgtcgc
     8881 gtcacaccgg aagcagggcc tccaccacag cggtgagctg ggcggccgtg cgacattcgt
     8941 gcatcggcag cacgtcggca tagcgcagcg ctgctgagtc gccgctgccc cactgccggc
     9001 gcggctccgg gttgagccag tgcgcgtgtc gcgcctgcgc caccagccgc ttgaccacag
     9061 ccaggttggg gtcccggtag ttggtgcgcg catcgccgag caccagcagc gagctcttcg
     9121 acgtcaccgc gtcgccccag tgctccgcga agctgccgaa ggagccgccg tagtcgctgt
     9181 ggccgtcaaa ggccacgaga tcggcctccc gcagcacccg ttgcatcacg ccggacagct
     9241 ccgcgcctgg gtcgaacagg tgggtcacct cgtccgcgcg ctccacgaac gcgaacaccc
     9301 gcaccttgct gaactgctcg cgcagcgcct gcacgaggag cagcgtgaag tggctgaacc
     9361 cggccaccga gccggagacg tcgcagagga tcaccagctc gggacgaccg ggccggcgcg
     9421 tgcggtacgc cgggcgcatc ggtaccccac cggtcgacat cgaacggcga agggtgcgcc
     9481 gcatgtccag gctgccgcgc ttggcgtgct tgcgccgcac cgctaaccgg gaggcgagcc
     9541 gccgcgccag cgggtgcacg gtgcgacgca gctgcgcgag ctgctccgcg taggcggaga
     9601 ggaactccac ctgctcgatc tgcttcggca ccgcggactt cgccacctcc tcacggcccc
     9661 ggatctcggc cgtgcgccgc cgcacctcgt ccgtgatcat cgcgcggaag gccgcgatcc
     9721 ggtcgcggat ctcccgacgc agcacctcgt cggagaacgc cgtgtcgtcg ccggtcgcgt
     9781 cctggagatc ggcgaggatg cgtgccagca acgtttccgg tgacagcgtc cgcagcgcct
     9841 ggtacgccga ccagctcggc cggccgcccg cgccctggat gcccgtgccg gccgcaccgg
     9901 agcgtccgag cgcgtccacc acggcacggg ccagctcggc gagcgcggcg gtgtcgccgt
     9961 cacgcagcag gtcggcgagg atgtcgcgca acgcgtccac gtcgaccggg ccgccctcgg
    10021 cgtcgtcgac ccgcggcacc tcgacctccc cgctgcccgc gcccagcgcg gccgggaagt
    10081 acagctcgaa cagcgtgtcg aacgcctgcc gctgccctga ccggcgcagc aacgccgctg
    10141 ccaggccttc gcgcacgtgc tcgcggtgga tcaggtcgag cgcgccgagc accctggcgg
    10201 cgtcgatggt ctcccccggt ccgacggaca ccccgtggcg gcgcagcgcg gcgacgaagt
    10261 cgaccaggtg tccgggcagg ccgtgctccg agctgatcct cgccgcggtg tccaccgcac
    10321 catcatggcc cgtcgatcag ttcagccgca gctcagcggc tgctttcacc gcgtccgact
    10381 ggtgcttcag taccacgccc agcgtggcgc gcatcgcctc gtcgtccagg gtgtcgaggc
    10441 cgagagcgag caacgtgcgg ccccagtcga tcgtctcagc caccgacggg gacttgcgca
    10501 gatccagccc gcgcagcacc cgcactgtgc ggaccagagc gtcgaccaac gactcggcga
    10561 gctctggcac cctgctcagc acgatccgac gctccaggtc ggcgtcggga aagtccaggt
    10621 gcaggaacag acaacgtcgt ttcagcgcct ccgacagctc gcgcgtggag ttcgaggtga
    10681 gcagcgcgaa cgggcgccgg gtggcgatga tcgtgcccat ctccggcacc gtcacctgga
    10741 agtcggacag cacctccagc agcagcccct cgacctcgac gtccgccttg tccatctcgt
    10801 cgacgagcag cacggtgggt tcgttcctgc ggatcgcggt gagcagcggc cgcggtagca
    10861 ggaactcctc cgagaagacg tcgtcgcgcg tctcgtccca ggactccttg ccctggcccg
    10921 cggtgatccg cagcagctgc ttggcatggt tccactcgta gagcgcgcgg gcctcgtcga
    10981 tgccctcgta gcactgcaac cgcaccagcc ccgagcccgt ggccgcggcg acggccttgg
    11041 cgagctcggt cttgccgacc ccggccggtc cctcgacgag cagcggcttg ccgaggcggt
    11101 cggcgaggaa gacggtggtg gcgacggccg tcgacgccag gtagccgacc ccggacaggc
    11161 ggtcggccac gtccgcgacc gaaccgaaca acggggactg gggagcgctc acgcggccca
    11221 tcctgcctgg cgacagcccg tgagcgaagc cgtgggccgg accggacgcg ccgtcgaccg
    11281 ggcggcgtcc gccgacgtgc attcaacccg tccaggcgga ggcccggcag gagttgtgac
    11341 ggcggttgtc cacaggcgga ctcccctgcc cgcccggctg tcggtgtcct cgtccacgat
    11401 gggttgcgtg acgacaggta tccccacgca gcaggatctc cgccggcggg ccgaggacgt
    11461 gctggccacc ctggccggtc cggccgcccg gctgcgggag gaccagtgga acgccatcga
    11521 ggcgcttgtc gcgcagcgca gacgggcgct ggtggtgcag cgcacgggct ggggaaaatc
    11581 ggcggtctac ttcgtggcca cggcgctgct gcgggcagcg ggcgccgggc ccaccgtgat
    11641 tgtctcgccg ctgctggcgc tgatgcgcaa ccagatcgac gcggcgcagc gggccggcat
    11701 ccacgcggtc accgtcaact cggccaacac ctccgactgg gagcgcacct acgcggccgt
    11761 cgacgccggc gaggtcgacg tgctgctggt cagcccggag cggctcaaca accccgactt
    11821 ccgcgaccgc gtgctgcccc ggctcaccgc gagcgccggg atgctggtgg tcgacgaggc
    11881 gcactgcgtg tccgactggg gccacgactt ccggcccgac taccggcggc tgcgggcgct
    11941 catcgccgag ctgccggcag ggatccccgt gctcgccacc acggccaccg ccaacgaccg
    12001 ggtggtcacc gacgtcgccg agcagctcgg gctgggttcg cacgatgcgg gcgacaccct
    12061 ggtgcttcgc ggctcgctcg atcgggagag cctgcggctg tccgtcgtac ggctgcccac
    12121 acccgcacaa cgactgggct ggcttgccgc gcagctcgac gccctgcccg gcgcaggcat
    12181 catctacacg ctcacggtct ccgcggccga ggaggtcgcg gagttcctgc gcgagcgggg
    12241 ccacccggtg gcgtcctaca cgggcaagac cgatccggag gagaggctgg ctgcggaggg
    12301 cgatctgctc gccaaccggg tcaaggcgct ggtcgccacc agcgcgctcg gcatggggtt
    12361 cgacaagccc gatctcgggt tcgtggtgca cctcggggcg cccgcctcac cggtcgccta
    12421 ctaccagcag atcgggcgcg ccgggcgcgc cgtcgaacgg gccgaggtgg tgctgctgcc
    12481 cggccgggag gaccgcgata tctgggccta cttcgcctcg ctcgccttcc ctccagagcc
    12541 gctggtccgc cagacgctgc gggtgctcag gcccgaaccg ctctccaccg ccgcgatcga
    12601 gacgcgcgtc gacctctcgc gtacccggct ggagatgctg ctcaaggtgc tcgacagcga
    12661 cggcgccgcg aagcgcgtca agggcgggtg ggtcaccacc gacgagccgt gggtctacga
    12721 cgaggagcgc caccgccgca tcgccgaggc ccgccgcgcc gagcagcagg cgatgctcga
    12781 ctacctcgac accgccgagt gtcggctggt gttcctccgc cgccagctcg acgacctcga
    12841 cgcacagccg tgcgggcggt gcgacaactg cgccgggccg gcgtggagca ccgacgtcga
    12901 cgcaggcgct gccacagcgg ccgaggaacg gctcacccgt ccgggggtcg aggtgccccc
    12961 gcggaaacag tggccgagcg ggatggccga gctcggggtg ccggtgtccg gccgaatcgc
    13021 cgcggcggag ctcgccgagg tcgggcgcgt gatcgggcgg ttgtccgata tcgggtgggg
    13081 cacgcggctg cgcgagctgg tcggggggac cgacgtgcct gtacccgccg acgtgctcga
    13141 cgcgtgtgtg cgggtgctgg ccggctgggg ctggacggag cggccggtcg gcgtggtcgg
    13201 gatcggctca cgcaccagac ctcaccagct cgatcacctg gcccgtcgga tcgccgagat
    13261 cggccgcctg ccgctgctcg gcaccctcgt tccggccggg ccgcgccccg gcgagcacag
    13321 caacagcgct cagcgcctgg ccgccgtgtg gggggcattc gaggcgccga cgttcgcacc
    13381 taccggcggg cccctcctgc tcgtggatga cctggtcgac agcggctgga cgatcacggt
    13441 cgccacgcgg ctgctgcggc aggccggggc gtcggcggtg ctgcccttcg cactggcagt
    13501 tgcgggctga gcgcgggccc gaccacgctc agttcgccgg cacgccgggc agatccacca
    13561 gtaggcagca gtgctccttg cgcaccacgc tcacccccgg cggcacggcc tcgccgaccg
    13621 ctccggcggg catcgcggcg atcctccatt ccgccgcccc cggccgtccc gccgccgtcc
    13681 accggtcgag cgaggcgagc agccgccggg cgagctctgg gccggccgcg ccgaacacgc
    13741 gcacggcaac cgagcccggc cggacgtcgg cgccggatcc cgctacgacc accgcgaggc
    13801 cgggcggccg ctcacccgct gtggtggcga gcgcgatcgt gccggcgccg ttgccgagcg
    13861 gcagcaggtc gtcggacagg ccggtctccg gggcgcacac cagcaaccgg cacagaccgg
    13921 gctcggtcag ggccagccag agaccgaagc cgtcccagac atccgcggtg cccagcgccg
    13981 ccggcaccgg gagcagcgcg cccggctcgg caagggctgc cgtcacgccg gccggatccg
    14041 cgccgggacc gtcctccgcg acctgcagga cgagtccgac gccgtccagc cgcgtaccgg
    14101 cgtccgagct cgctcccacg ccacggagcc ggatgaaggc gcagctgcgc accgagtcgc
    14161 tgtgcagcat cccttcgggg ccgaggtcga aggctgtgga gagctggctc ccgcgcacgg
    14221 cgagcggcag cagcaggcga ccgcctgggc ccagctgggc cacccactcg ggacggatgt
    14281 cgctgctgcc gacggtgagc acgacgcggt cgtagggcgc gcccgcggga tggccgagtg
    14341 ctccgtccgc cgcgaccagc tcaactcctg acacacccgc ctcggccaga tgcagagcgg
    14401 cccgggcgat gaggtcctcg tcgatgtcga cggcggtcac cgcacccgcc gggccgacga
    14461 ttcgcgccat cagcgcggcg ttgtagccgg tgcccgcccc gatctcgagc acgtgatgcc
    14521 cgggtcggag gtccagctgc tccagcatga tcgccatcat cgacggctgc gacgccgagc
    14581 tcgtcgcgac gccgtcggag aactggacgg cgaccgcctc gtcggcgtac gcctcgtcga
    14641 gcgccagccc cggcaggaag acgtggcggg gcactgctcg gaacgcgtcc tcgacggcgg
    14701 gcgaacgaat ccggtcgccg tcacacagga cgtcgaccat ccgggcgcgg gcacgatcgg
    14761 cggcagccat cggtcgaagt cttgtccggg caccctcagg gcgacaagcc gaaacggaca
    14821 actcgcgggc gaagaacgag cgacggcagg cgcagaacga gcgactcgca ggcccgaacc
    14881 gggcgacccg cgggcgcaga acgggcgact cgcgggcggg gaaggggcga ctcgcgggcg
    14941 caatacgggc gactcgcggg cccgaaacgg gcgactcgcg gggtcagggg gtcgttaggg
    15001 ggaccaggtc gtcggcgtgc accacctcgc ggcgctgctc gggcgcgagg tcgcgggagc
    15061 ggcggccgat cagcgcgggg agctcggtgg cgtcgaagcc caccaccccg cgggcgacga
    15121 ccacgccgtg cggccccacg aggtccacca cgtcaccggc cacgaagtcg ccggagatcc
    15181 cgtggatgcc ggcggcgagg agcgagcggc gacggtccat gaccgccgcc acggctccgg
    15241 cgtcgaggtc gagggagcca cgcacgtcgg ccgcgtgcaa cagccagaac cggcgggccg
    15301 acatccgacg tccggacggg cggaaggcgg tgcccacctt gggccccacc tccgaggcgt
    15361 ccagcgcgga ggtgacgtcc tcggccgcgg cgagcagcac cgggatgccg gcggcagcgg
    15421 ccatcgccgc tgccgtgatc ttggtggcca tcccgccgcg accgaggctg ccggcgccgg
    15481 gtcgggccac ccgcaccgac tccaggtctg atggcgcgtc cacctcgccg agcagccgcg
    15541 agccgggcag gcgggggtca ccgtcgtaga gcccgtcgac gtcggacagc agcaccaggc
    15601 cgtcggcgcc gatgaggtgc gccacgagcg cggccagtcg gtcgttgtca cccaccctga
    15661 tctcgtcggt ggccaccgtg tcgttctcgt tcaccaccgg cagcacgtcg agaccgagca
    15721 ggcgctccag cgtgcgctgg gcgttgcggt agtgggcgcg gcggatcatg tcgtcggcgg
    15781 tgagcagcac ctggccgatc gcctgcccat gccgcccgaa tgacgccgca taggcctgcg
    15841 cgagcagcag ctgcccgacg gatgccgcag cctgctgggt ggcgaggtcg cgcgggcggc
    15901 gggccaacct gagcggggcg agcccggccg cgatcgcacc cgacgacacc agcaccacct
    15961 ggccacccgt agcgcgccgg gtcgcgagcg cgtcgaccag cgcatcgagt cgcagcgggt
    16021 cgagtcctcc gtcgaggctc gtgagcgacg acgacccgac cttgacgacg atccggcgcg
    16081 cagcggccag ctgtcgccgg gcggtcacgg ccgggtccgg ggccgttccc cggactccac
    16141 aggctcgtcg tcccacgtcg cgtcgtccca gcgcgggccc gcggggtcgt cggacgactc
    16201 gtcggcgagc agctcctcgt cggtgcgatg cttgcgccgc tcgcctcggg ccgccttgcg
    16261 ttcggccgcg cggatgcggt cgtcgcgctc cagccgccgg tcggtgccgc gaccggacat
    16321 cagcacggcc accccagcgg gcgtgctcgg ctcccagtcg aacgtgacat cgccgatcgt
    16381 gaccgggcag ccggcctcgg cgccggcctg ggccagcgcc tcctccacgc cgagccgggc
    16441 gagccggtcg gcgaggtacc cgacggcctc gtcgttctcg aaggacgtct ggcggatcca
    16501 ccgctcggga cgggcaccgc gcacgatgaa cccgccgggc agctcgcggt cggcctcgac
    16561 ggtgaagccg gcatcgtcga ccgcctgcgg ccgcagcacg atgcgggtgg gctcgaggat
    16621 cggctgggcg gcgcggtagg cgcgcacctg ctccgccatc gcgaacgaca gctccctcaa
    16681 gcctgcgcgg ctggcggcgg agatcgggaa gacgggccag ccgaagcgct cggtcagctc
    16741 ggccgtgacg agctcggcca tgtcctgcgc atccggcacg tcgatcttgt tgagcgcgat
    16801 cagccgcggc cggtcggaca gctccccgcc cagtgcgggg gtgtagcggg ccagctcgtc
    16861 ctccaatgcc tggacgtcgg acacgggatc gcggccgggc tcgaaggtgg cgcagtcgac
    16921 gacgtgcacg agcactgagc agcgctcgat gtgacgcagg aagtccagcc ccaggccgcg
    16981 gccctcggac gcccccggaa tcaggccggg aacgtcggcc acggtgaaga cgtcctcgcc
    17041 tgcggtgacc accccgagct gcggcacgag cgtggtgaac gggtagtccg cgaccttcgg
    17101 cctcgccgcc gagagcgcgc tgaccagcga ggacttcccg gcgctgggga acccgagcag
    17161 cccgacgtcg gccatcgagc gcagctcgag cacgaggtcg agggtgtcgc ccggctcgcc
    17221 gagcagcgcg aacccggggg ccttgcgggt gggg
//
LOCUS       DASRSC010000169        31779 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2676412,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000169 DASRSC010000000
VERSION     DASRSC010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 31779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31779
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2676412"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..70
                     /gene="rpsH"
                     /locus_tag="VFE39_08285"
     CDS             <1..70
                     /gene="rpsH"
                     /locus_tag="VFE39_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET6258634.1"
                     /translation="GLQTDRQAKKNGVGGEVLAYVW"
     gene            83..622
                     /gene="rplF"
                     /locus_tag="VFE39_08290"
     CDS             83..622
                     /gene="rplF"
                     /locus_tag="VFE39_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006245132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET6258635.1"
                     /translation="MSRIGKLPITVPSGVDLTIDGRTVTVKGPKGILSHTVIEPITIE
                     RDETGTVVVKRPDDERRNKAMHGLSRTLVNNLVVGVTNGYEKKMEIYGVGYRVALKGR
                     TLEFALGYSHPIVIEAPEGITFTVESPTRFSVAGIDKQLVGEVAAKIRKLRKPDPYKG
                     KGVRYAGEVIRRKVGKTGK"
     gene            625..1029
                     /gene="rplR"
                     /locus_tag="VFE39_08295"
     CDS             625..1029
                     /gene="rplR"
                     /locus_tag="VFE39_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET6258636.1"
                     /translation="MAETISANARKRTASQVSNQRRTARTRRHARLRKKVSGSAERPR
                     LAVNRSARHIVVQLIDDLAGHTLASASTMEADVRTLEGDKKARAAKVGELVAARAREV
                     GVTAVVFDRGGYNYHGRIAALADAAREGGLEF"
     gene            1067..1714
                     /gene="rpsE"
                     /locus_tag="VFE39_08300"
     CDS             1067..1714
                     /gene="rpsE"
                     /locus_tag="VFE39_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET6258637.1"
                     /translation="MPGRARRDGGGPSTGPGGERSNSGDRRDRRDGGRGGGGQDKTPY
                     IERLVTINRVAKVVKGGRRFSFTALMIVGDGDGLVGVGYGKAKEVPAAIAKGVEEAKK
                     SFFRVPRIGGTIVHRVQGEAAAGVVLLRPASPGTGVIAGGPVRAVLECAGIHDVLSKS
                     LGSDNAINVVHATIAALKMIQRPEEVAARRGLPLEDVAPAQMLRARAAAAQGARS"
     gene            1714..1947
                     /gene="rpmD"
                     /locus_tag="VFE39_08305"
     CDS             1714..1947
                     /gene="rpmD"
                     /locus_tag="VFE39_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET6258638.1"
                     /translation="MAQTSSDATAQSTPTSDTKLVVTQVRSTIGVKANQRATLKSLGL
                     RKIRQTVERDDTPQVRGMIHTVRHLVTVEEVGS"
     gene            1947..2399
                     /gene="rplO"
                     /locus_tag="VFE39_08310"
     CDS             1947..2399
                     /gene="rplO"
                     /locus_tag="VFE39_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET6258639.1"
                     /translation="MSTTIKIHHLRPAPGAKTAKTRVGRGEGGKGGKTAGRGTKGSKA
                     RKNVPAGFEGGQMPLHMRLPKLKGFKNRFRVEYQVVNVGDLAQLFPAGGAVGPDELAE
                     AGAVRRNQLVKVLGNGDIDGIALTVSAHAFSGSARDKITAAGGTVTEL"
     gene            2584..3891
                     /gene="secY"
                     /locus_tag="VFE39_08315"
     CDS             2584..3891
                     /gene="secY"
                     /locus_tag="VFE39_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET6258640.1"
                     /translation="MLSAFRSALTTPDLRKKILFTLGILAVYRLGTAIPSPGVSYPNV
                     QECIRQVQGSSNSGIYSLINLFSGGALLQLSIFALGIMPYITASIIVQLLTVVIPRFE
                     QLKKEGQSGQNKLTQYTRYLTIALAILQATGIVALADRGQLFGDCSLPVIPNSGIYQL
                     SVIVIVMTAGTAVVMWLGEQVTERGVGNGMSLLIFTSIASRIPAEGANILSNGALVFI
                     GVCVFGLAIIASVVFVEQGQRRIPVQYAKRMVGRRMYGGTSTYLPLKVNQAGVIPVIF
                     GSSLLYLPDLVSRLAGSTGGGFQQFVQTYLVDQSSWVHITLYIALIIFFTYFYVGITF
                     NPEERADEMKKFGGFIPGIRPGRPTAEYLNFVLSRITLPGSLYLGIIAVLPNFFLGIT
                     GSGQNQNFPFGGTAVLIMVGVGLDTVKQIESQLMQRRYDGFLR"
     gene            3945..4493
                     /locus_tag="VFE39_08320"
     CDS             3945..4493
                     /locus_tag="VFE39_08320"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET6258641.1"
                     /translation="MRLVLVGPPGAGKGTQAVRLAERLDVPHISTGDLFRANLKDETE
                     LGREAKRYMDAGDLVPDAVTVAMVRDRLTHDDAAKGFILDGFPRTLRQAASLGELLAE
                     RGEDLDAVVEFQVAEDELVRRLMGRGREDDTEDVIRRRQQVYRDETAPLLGHYSDRLV
                     TVDAVGTVEEITDRVANALRSR"
     gene            4490..5317
                     /gene="map"
                     /locus_tag="VFE39_08325"
     CDS             4490..5317
                     /gene="map"
                     /locus_tag="VFE39_08325"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET6258642.1"
                     /translation="MSGGRSGVRGLLARWRGRDIELKSPEELEAMRAAGALVATTLVA
                     VTKLARPGVSTGELDELAEQVIREAGAVPSFLGYHGYPASICASVNEQIVHGIPAATQ
                     VLADGDLVSVDCGAILDGWHGDAAVTILVGSVSDADRALSAACEAALYAGIAAVQPGG
                     RLSDISHAVQVASETAGKRDGVSYGIVAEYGGHGIGTSMHMDPFLPNFGDPGQGPRLV
                     PGMALAVEPMLTAGDPETRELEDGWTVVAADGSRAVHWEHTVAITDNGPRILTVLPD"
     gene            5470..6570
                     /locus_tag="VFE39_08330"
     CDS             5470..6570
                     /locus_tag="VFE39_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET6258643.1"
                     /translation="MPRESAAGERRVALVPKVVEKLRSRGVEVVVEAGAGLGALIPDE
                     LYIAAGATVGDPWSAEVVVKVAPPSPEEVEHLRSGSTLIGFLAPLTARETIAALKAAG
                     VQAFAMEAIPRISRAQSMDALSSQGNVAGYKAVLLAAEKSTRFFPMLTTAAGTVKPAT
                     ALVLGVGVAGLQALATAKRLGARTTGYDVRPEVADQVRSLGAQWLDLGIEAVGEGGYA
                     RELTDDERAEQQQRLEKAITGFDVVITTANVPGRKAPTLVTAAAVEGMRPGSVVVDLA
                     GESGGNCELTEPGEVVVKHDVTIASPLNLPATMPEHASELYSRNVSALLELMLKDDGD
                     GGVTLAPDWDDEVLAKSCVTRVEATAASGGEA"
     gene            6570..6914
                     /locus_tag="VFE39_08335"
     CDS             6570..6914
                     /locus_tag="VFE39_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HET6258644.1"
                     /translation="MESGLLSNIAILVLAGFVGFAVISKVPNTLHTPLMSGTNAIHGI
                     VLLGGLIVLGHLENPSVIDQIILVIAIVFGMINVIGGFLVTDRMLGMFKAKPEPRQAP
                     SAEKAGTNGKGH"
     gene            6914..8296
                     /locus_tag="VFE39_08340"
     CDS             6914..8296
                     /locus_tag="VFE39_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HET6258645.1"
                     /translation="MATLVPILYIIAFALFIYGLMGLTGPKTAVRGNLIAAVGMTIAV
                     VATLLEVRHNWVLIIVGLVIGAALGVPSARQVKMTAMPQMVALFNGVGGGAVALIAWS
                     EFRTSDGFLAEPGYVVVATLFAAIVGSISFWGSLIAFGKLQEILPGRPIGLGKAQQFV
                     NGLLLLAAVACAVVAGLGGAPQIWIIGLLVLAAVLGVLVVLPIGGADMPVVISLLNAL
                     TGLSAAAAGLALNNTAMIVAGMIVGASGSILTNLMATAMNRSIPAIVAGGFGGTTTVP
                     GADDGVDRTVKSTSAADAAIQMAYASQVIVVPGYGMAVAQAQHAVKDMAKLLEAKGVE
                     VKYAIHPVAGRMPGHMNVLLAEADVSYDALKEMDDINDEFSRTDVTLVIGANDVTNPA
                     ARTDPSSPIHGMPILNVDQSRSVIVLKRSMSSGFAGIDNPLFYADNTSMLFGDAKKSV
                     AAVTEELKAL"
     gene            complement(8358..8549)
                     /locus_tag="VFE39_08345"
     CDS             complement(8358..8549)
                     /locus_tag="VFE39_08345"
                     /inference="COORDINATES: protein motif:HMM:NF016234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HET6258646.1"
                     /translation="MTGIAQLGRGLLWYLRELTGETQYDRYVERHAREHAGTAPLSRR
                     EFERRRIDGMDPKPGSRCC"
     gene            complement(8546..10696)
                     /locus_tag="VFE39_08350"
     CDS             complement(8546..10696)
                     /locus_tag="VFE39_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HET6258647.1"
                     /translation="MTLSELRSRMTPRSMLIWGAVAVVGAVCWGVLALSRGEQISAIW
                     LVGAALGSYAIAYRFYARFIAKKVLQVDDNRATPAERLDNGTDFQPMDRRVLFGHHFA
                     AIAGAGPLVGPVLAAQMGYLPGTIWIIVGVIFAGAVQDMVTLFFSMRRDGRSLGQMAR
                     DEIGPIGGVAALIAVFAIMIILLAVLALVVVNALAKSPWGTFSIAMTIPIALFMGYYM
                     RVIRPGRVIETSLIGVALLLLAIVAGGWVQNSSWAAAFTLDPTTLVFCLVIYGFAASV
                     LPVWMLLAPRDYLSTFMKVGTIALLAVGVLITAPLLKNAPVTEFAFSGKGPVFAGSLF
                     PFVFITIACGALSGFHALIASGTTPKLIQKESQVRFIGYGAMLLESFVAIMALIAASV
                     LDPGLYFAMNAPAGLLGTTVESASQAVNNLGFTITPEALAAAAASVQESTLIARTGGA
                     PTLAVGMSEIFSGAFGGDAFKAFWYHFAIMFEALFILTTVDAGTRVGRYMLQDTLGNV
                     YKPMSNVNWKPGMYLTSAVVVGAWGYFLYAGVTDPLGGINQLFPLFGIANQLLAAVAL
                     TVCTTLLIKTGRAKYAWVTAVPLAWDAAVTLTASYQKVFSPDPTLGFFAQREKYAAAL
                     DQGQVLAPAKNIADMQAVVTNSTVDGVLAALFAILIIIVIANALLVWYRALTSSEPLP
                     STEAPAVPSQIVAPSGLIPTAEEKRALATAGERS"
     gene            complement(10955..12265)
                     /locus_tag="VFE39_08355"
     CDS             complement(10955..12265)
                     /locus_tag="VFE39_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6258648.1"
                     /translation="MDDMPEEAADGRLRLPRPLRPFRHREYRLLATSMAASLFASGLW
                     LVAIVYQVIALDGGPSELSVVAAASSVGLLVSVLIGGVAADRLPKRAVMIAVEAVRIA
                     SAGTAGVLGVTGALQLWHLAVIAFVVGAAEAFFYPAYSAILPTLLPPDELLAANGVEG
                     TLRPIAQQALGPALAGVLVGAFVPGISLLLAAGIYALALVALLAMRTAPVTPMEAGNS
                     VLADLVEGFRYLFRTGWLFATLAFATLYVLVLIGPIEVLLPFAVRDQVGGGASSFALV
                     LGAFGIGGAVGSIVVSSWRLPRRYLTAMILLWGAGAAPLVLIGVTRHLWVMAAATFVV
                     GFTGAVATVIWGTLLQRRVPPHLLGRVSSLDFFVSLALMPVSMALAGPVGEWLGVPMT
                     FVLAGAVPVFLAVAAIVGWRLPADEIAHPLDAPVTQSNTPAEKA"
     gene            12391..12870
                     /locus_tag="VFE39_08360"
     CDS             12391..12870
                     /locus_tag="VFE39_08360"
                     /inference="COORDINATES: protein motif:HMM:NF019656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HET6258649.1"
                     /translation="MDDAAEPGRPGEVRPEDMRAGDADRERVLDRLRTAHVEGRLTTD
                     EFDERITATLAARTYGELGAITIDLPGEAPAPPTVVESAAPAATLAEYRDVDALQERD
                     ARGAVIGWAGASVTTIAIWAMSCIATASFVYPWWIWVAGPWGLVLLSSWVRDRRHEH"
     gene            13046..13267
                     /gene="infA"
                     /locus_tag="VFE39_08365"
     CDS             13046..13267
                     /gene="infA"
                     /locus_tag="VFE39_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET6258650.1"
                     /translation="MAKKDGAIEVEGRVVEPLPNAMFRVELENGHRVLAHISGKMRQH
                     YIRILPEDRVVVELSPYDLTRGRIVYRYK"
     gene            13299..13412
                     /gene="rpmJ"
                     /locus_tag="VFE39_08370"
     CDS             13299..13412
                     /gene="rpmJ"
                     /locus_tag="VFE39_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET6258651.1"
                     /translation="MKVQPSVKKICDKCKVIRRHGRVMVICENLRHKQRQG"
     gene            13602..13982
                     /gene="rpsM"
                     /locus_tag="VFE39_08375"
     CDS             13602..13982
                     /gene="rpsM"
                     /locus_tag="VFE39_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET6258652.1"
                     /translation="MARLSGVDLPRDKRMEIALTYIFGIGRTRSIQILEATGIDRDLR
                     SKDLTDEDLAALREYIETNYRVEGDLRREVQADIRRKIEIGCYQGIRHRRGLPVRGQR
                     TKTNARGRKGPKKTVAGKKKAGKK"
     gene            13982..14389
                     /gene="rpsK"
                     /locus_tag="VFE39_08380"
     CDS             13982..14389
                     /gene="rpsK"
                     /locus_tag="VFE39_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008747488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET6258653.1"
                     /translation="MPPRTRSASGPKKVRRKEKKNVAHGHAHIKSTFNNTIVSITDPT
                     GAVIAWASAGHVGFKGSRKSTPFAAQMAAESAARKAAEHGMKKVDVFVKGPGSGRETA
                     IRSLQAAGLEVGTISDVTPQPHNGCRPPKRRRV"
     gene            14407..15012
                     /gene="rpsD"
                     /locus_tag="VFE39_08385"
     CDS             14407..15012
                     /gene="rpsD"
                     /locus_tag="VFE39_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET6258654.1"
                     /translation="MARYTGPMTRKSRRLKVDLVGGDQAFERRPYPPGQHGRIRIKET
                     EYLLQMQEKQKARFAYGVLEKQFRRYYEEANRRTGKTGDNLLQILETRLDNVVYRAGL
                     ARTRRMSRQLVNHGHFMVNGHKVDIPSYRVSPYDIIDVRPKSLGTDPFIIAKELVGDR
                     PIPGWLQVVPSTLRILVHQLPERAQIDTQVTEQLIVELYSK"
     gene            15115..16158
                     /locus_tag="VFE39_08390"
     CDS             15115..16158
                     /locus_tag="VFE39_08390"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET6258655.1"
                     /translation="MLISQRPTLTEDSVVDTRSRFVIEPLEPGFGYTLGNSLRRTLLS
                     SIPGSAVTSIRIDGVLHEFTTVPGVKEDVTDIILNLKELVVSSEEDEPVTMYLRKQGP
                     GPVTAGDIVPPAGVTVHNPDLHIATLNDKGRLEVELVVERGRGYVPAMQNKVTGAEIG
                     RIPVDSIYSPVLKVTYKVEATRVEQRTDFDKLVLDVETKPSITPRDALASAGKTLVEL
                     FGLARELNVDAEGIEIGPSPQEADTIAAFAMPIEELDLTVRSYNCLKREGIHTVGELV
                     GRSEADLLDIRNFGAKSIDEVKMKLVGLGLALKDSPPGFDPSAAAAEYGSDGFDPETF
                     GDDGQDYAETEQL"
     gene            16161..16889
                     /gene="rplQ"
                     /locus_tag="VFE39_08395"
     CDS             16161..16889
                     /gene="rplQ"
                     /locus_tag="VFE39_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET6258656.1"
                     /translation="MPQPTKGPRLGGSPAHQRLILANLATALFEHGRITTTEAKARRL
                     RPYAERLITKAKRGDLHNRREIQKVIRDKTVVHRLIADIGPFFADREGGYTRITKTLP
                     RKGDNAPMAVIELVQQKTVTDEAERARRVASSQQKAAAAPAPAETETAAAPAEDTAGE
                     ESTSTAEAPYGEGSHGPLDDPTEAPEGFPIKGNEDSKLYHVPGSSHYDPTEAEVWFAS
                     AEAAEAAGFELPPSQREDDSTEES"
     gene            16973..17815
                     /gene="truA"
                     /locus_tag="VFE39_08400"
     CDS             16973..17815
                     /gene="truA"
                     /locus_tag="VFE39_08400"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005461396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET6258657.1"
                     /translation="MGEGATRGRETGDSRRFRLDLGYDGTEFSGWAMQPGRRTVCGVL
                     TEALSTVLREPVSLTVAGRTDAGVHATGQVAHADLPAELDAPWLVRRLARLLPSDVRV
                     RAVTPVPAAFDARFSALRRHYRYRVATAPHGAEPLRARDTLSWPHPLDLAVVVAASDR
                     LLGEHDFAAFCKRREGATTVRALQRLSWASADDGVVEAAVSADAFCHSMVRSLVGALL
                     DVGRGRRAVAWPAELLARRERASEVPVAPAHGLTLVAVDYPPPAELAVRAAATRRLRV
                     PGAS"
     gene            17981..18445
                     /locus_tag="VFE39_08405"
     CDS             17981..18445
                     /locus_tag="VFE39_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET6258658.1"
                     /translation="MFDPPVSDPAVLLDVALAEARAGLAEGGIPIGAALFAADGTLLG
                     SGHNRRVQDDDPSMHAETAAFRAAGRQRDYRSTIMVTTLSPCWYCSGLVRQFGIGSVL
                     VGEARTFHGGHEWLAEHGVAVTVLDDDRCADLMADFIAARPELWHEDIGEPS"
     gene            18442..19401
                     /locus_tag="VFE39_08410"
     CDS             18442..19401
                     /locus_tag="VFE39_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HET6258659.1"
                     /translation="MIPTIDLAAWRAGDAATGPAVDSALQRAGFLLVTGHGVDAALRA
                     AVRGAARRFFALPAEVKARYAVRIGGRGWLAPGVEANGNVEGTETPPDLKESFAVGPD
                     TPTGDPEVDGVWFPPNVWPAEVPELHHAVDRYLAAMTAVSRDVLELCADALRLPRETL
                     TALAAHPTWTFNINRYPPLTEVGQPAPGQYRIGPHTDFGTVTVLDREPGAGGLQVDCA
                     DGNGDGTGWADAPYDPAALTVNIGDLLAHWTGLRWRSGRHRVLPPQPDSPHEELISLV
                     FFFELDHDALVTPLAPPIGRRTDLPPVVSAPFLRERLDAISVG"
     gene            19460..20899
                     /gene="eccB"
                     /locus_tag="VFE39_08415"
     CDS             19460..20899
                     /gene="eccB"
                     /locus_tag="VFE39_08415"
                     /inference="COORDINATES: protein motif:HMM:NF016964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccB"
                     /protein_id="HET6258660.1"
                     /translation="MPRASAVRAPATRDQVDAYRFGLRRLEAALVRGDPVPLHEQVRS
                     QRRAALAGVVLGLLGLCGAAALAKVVPEPNWTQQAVIVGAGSGAMYVVAHGPDRLVPV
                     ANLPAARLVLAALRSGGSTGADPATATPVMVPDAALDTAARTPTAAVPGAVAVRPDGP
                     PVPPRWAVCDRTTADGDLVRTTVVAGMAPPEPMAPGDGVLLDGPDGATWLVTAGHRHR
                     VDTGDGKVMAAFGLTARVPRAAAPDLVSLLPEGPVLATPEVPGRGGPAPRGVPGRIGD
                     VLVAHPVGAAARLFVVLAGGLQEVPDVVAELLRVASGASRALPIGADVLAGAAFVDHV
                     RVDGWPVGAPRLREPAEAPVTCWTWTANGEPAGDVWVGLAEPLPATTLPVAFAQADGP
                     GNRVDAVAVGPGGAVRATGPGRPPGAGPVWLVAATGVTFGVADDATAAALGITAADPA
                     PEAALRLLPTGPALDLAAAGRVVDVLPFG"
     gene            complement(20921..22234)
                     /gene="mycP"
                     /locus_tag="VFE39_08420"
     CDS             complement(20921..22234)
                     /gene="mycP"
                     /locus_tag="VFE39_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion-associated serine protease
                     mycosin"
                     /protein_id="HET6258661.1"
                     /translation="MRRAACAVATALALTCPAVPAAAEVPPLPPPVRVEPPAGRPPGP
                     APGLHAPERCTPLPGDDGPADDEAVDDETGSRLRLSDVHRLATGRGVLIAVIDTGVFP
                     HRLLGDRLRGGGDYLTGGDGLDDCDGHGTAVAGLLAAAAGPGPGGRSDAPIGIAPGAR
                     LLAIRQSSPSFEVPGPDGTSHPAGDTETLAEAVVRAVREGADVINISEAVCLAAGQAA
                     VAGAGLHAALRFAADADVVVVVAAGNVGSGSCTEPGSGQVSLPGWYDDELLAVGAVGP
                     DDAPAPFTVPGPWVDVAAPGTGLRSLAVGGGTTTTGVDGTSFAAPWVAGLAALVRERF
                     PELSAKQVADRILVTARRPARGRDDRLGHGVIDPVAALTAVPAVLEPTARPVSGVAVA
                     ELAGTTPRPAAAPSSSQIDFVAAGLLLVTGAVAAALLRRRSRLPR"
     gene            complement(22231..23619)
                     /gene="eccD"
                     /locus_tag="VFE39_08425"
     CDS             complement(22231..23619)
                     /gene="eccD"
                     /locus_tag="VFE39_08425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion integral membrane protein
                     EccD"
                     /protein_id="HET6258662.1"
                     /translation="MRATAADGTYCRLTVLAPRARVDVALPADVPVAELVPMVLELVG
                     EPVFGLRPQPWRLSGAAGGPLPSGATLGELDVLDGELLRIAPEGTAPVPPVFDDPVDA
                     LAATAGTAGAADHRFGAAGVLAVSVAAAALLAGGPGAAGTLATVLVAALAAAVAVAHA
                     ARLARRATSDEHNGGSLHLSARTAAFAAVPLAAAAGWAALSHSSGAAQLLLATAAAGI
                     TAAVAQIALRVVAPVLVGTVVAAMPVGAATMVVLRLGVDPAAVAAGFGAVALVIGPLL
                     PRAALRLAGLPQPVVPADGGELVDADNGPDRLPPAELAERADLARGYLAGLAGGCAVV
                     AAAATLPVAGAGGWAGPTLAAVTVAVLALRARSFADRLPARTLLASAITAAVALAGLV
                     VVQAGAAARPVAAGALLVAAAGGALILGRTRPMGSPVSRRAVDLLEGVLVAAAIPLAL
                     AAMDLFRLVRGL"
     gene            23774..27727
                     /gene="eccCa"
                     /locus_tag="VFE39_08430"
     CDS             23774..27727
                     /gene="eccCa"
                     /locus_tag="VFE39_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EccCa"
                     /protein_id="HET6258663.1"
                     /translation="MGTVGVVRPRRLPPRMPGGELTLEPPPEPERVVPAGVLARLLPA
                     VMLLGSVGFVAVLGVHNPTSWLFGGMFAISTLSMMMSGSGRGGGTRSASLDEDRRDYL
                     RYLSLLRGRVRGIAAEQRAVLESVHPDPAAWPAVLACDRIWERRPADADFGQLRIGRG
                     AQRLATRLVAPQTGPVEGLEPVTALALRRFLLGHAVVPDLPVALSLRASSTVWLEPAE
                     PAAGLGPARGLARALVAQYALWHSPADALLAVVAPPTLAPEWEWVKWLPHLAHPRRRD
                     AVGPLRMITDDADDVRRWWVAELAGRPAGPGMGEPHLLVVVDEVAKGPGPWAGVAGVT
                     VLRVGAPPGRRPGPSVVRLIVGTDGLYRIGSADPARHSEPASANEQPTWIGRPDGLVV
                     AEARALARRLARYRPGGAEPAGDTTPRSALGLPTLLGLEPGPAGIAALRARWARADTD
                     RMRVPIGVDERGGPVTLDLKESAQGGSGPHGLCVGATGSGKSELLRTLVLGLAATHSP
                     AELNLVLVDFKGGATFLGLAGLPHVSAVITNLADELTLVDRMADALAGEITRRQELLR
                     SAGNLVGVADYAAARRSGADLPPLPALFVVVDEFSELLAQRPELLELLVTIGRLGRSL
                     GIHLLLASQRLEEGRLRGLESHLSYRIALRTFSAAESRAVLGVPDAHQLPSAPGSAFL
                     ATGTDELVRFRAAYVSAPGTAPPPPGVAAGRARAYPFRAGPVPVAAPAELVVPAEPDG
                     ADGPTVLDTVIDAMAGQGLPAHRVWLPPLDAPPPLDEVLGPVRPVPGRGLDAGRQALL
                     RVPVGLVDRPYQQRRDQLLIDLAGGSGHLAVVGGPRTGKSTALATTVLGLALTHTPDE
                     LGVHVLDFGGGALIPLAGLPHVGTVADRQEVDLVRRTVAELSAVLARRERLFRDAGIA
                     SVEEFRVRRAAGAHPGEPSTDLLLVVDGYLTLRREFDDLEARLLPLAAQGLSYGLHLA
                     VSATRWSELRPALKDLLGGRVELRLGEPSESEVDRRRAAEVPARPGHGLAPDGAPAVL
                     AAPRLGAGATDQAGLVAAIAAAWPGPGVPPVRLLPERIDYNRLPATGTGATGIPIGVD
                     EERLDRVEIDFAAEPHLTCFADAESGKTNMLRLVARGVTSRHGPEAARVVVVDHRRTL
                     LGVVPDTHLIGYASTVDATAEVAREVAASLRGRLPGPDVTPRQLRERSWWTGPDVYVL
                     VDDYDLVAPGGGAQHPLLPLVGFLPQAKDVGLHLVVARRCGGAGRALFDPLLGRLREL
                     AAPGLVMNGSPDEGALVESVKPAPLPPGRATLVDRRRGARRIQLAWLPPDAEDGL"
     gene            27816..29360
                     /locus_tag="VFE39_08435"
     CDS             27816..29360
                     /locus_tag="VFE39_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR03931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion-associated protein"
                     /protein_id="HET6258664.1"
                     /translation="MSGTPVRVAVQLGAATARLAAVGHDGEPWLAAEATAAGTTVPSL
                     LAELVPCPEELVLVHPAGRSASRAPAWMGEGLGLAGHVRAVSAPLAAAGRGDVVVLDV
                     GHAGAEVTRLDTHGQVLALQARPVGGAALDALLVELLGLSMAAPAALAEARRVREALS
                     LLPSVEVRTPECLRVGASEVRAVLARPLGAVIEALREVLRRTGPAPVLLVGGLARMPL
                     LAELVDEAGIADVSVAPRPDAAAVLGALTLPATAGLTIGPRGFAAPRPGAAGSPRSSG
                     AGSERCPRAAPATPVRPGPDRPRPIGPSVAGPELTTGGDRRQVPARRLPPLPPRTRRP
                     LLRTALLAAAAVGLIAGLLGVGALLPSGPAAATVPAGVLVQYGYRLDIPSGWEHTGGL
                     PERRRILLTRAGAPEGSDLIAVERTPLGYDTGAEPERAQAELRAEFDAAAGGSALSGY
                     RDARIAGREATSYRQQEADARTVVDWFVLLDGDAQLSVGCRYTATGNETVLAACAVVI
                     ASLRRA"
     gene            29817..30128
                     /locus_tag="VFE39_08440"
     CDS             29817..30128
                     /locus_tag="VFE39_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HET6258665.1"
                     /translation="MADGFGTTVEEMQRAGRHVFAVNDTVQADLATLRGRLGPLAGAW
                     RGEASTAFTGLMARWDADARALSEALRTIGEAIQGTGASYQAQEEQHSGELSAIRAAL
                     G"
     gene            30139..30426
                     /locus_tag="VFE39_08445"
     CDS             30139..30426
                     /locus_tag="VFE39_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HET6258666.1"
                     /translation="MSEIKVTFGALEAARADVGGTATRISGQLEELKRFLAPMAATWE
                     GQAAAEYQVTQRRWDTAAADLAAVLAQIGLALGTANESYRQVEQANAARWR"
     gene            30655..31098
                     /gene="rplM"
                     /locus_tag="VFE39_08450"
     CDS             30655..31098
                     /gene="rplM"
                     /locus_tag="VFE39_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET6258667.1"
                     /translation="MPTYSPKPGEITRAWHVIDATDVVLGRLATHAATLLRGKHKPTY
                     APHMDTGDFVIIVNAEKIAVSGNKRDGKFIYRHSGYPGGLRQRSVGEMIEKHPDRLVE
                     KAIKGMLPKNRLGRAMSKKLKVYAGPDHPHAAQQPAAFEITQVAQ"
     gene            31284..31706
                     /gene="rpsI"
                     /locus_tag="VFE39_08455"
     CDS             31284..31706
                     /gene="rpsI"
                     /locus_tag="VFE39_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET6258668.1"
                     /translation="MEAVPAIPAVFSDRPIQTVGRRKEAVVRVRLMPGSGAFRLNGKS
                     LEEYFPNKLHQQLIREPLVTVEKTERFDIFANLHGGGTSGQAGALRLAIARALIESDP
                     EDRPPLKKAGFLTRDPRVIERKKYGLKKARKAPQYSKR"
BASE COUNT         4624 a        11333 c        11581 g         4241 t
ORIGIN      
        1 cggcctgcag accgaccggc aggccaagaa gaacggcgtg ggcggagaag tcctcgccta
       61 cgtctggtga gggggtaaca ccatgtcccg aatcgggaag ctgcccatca ccgtgccgtc
      121 cggggtggac ctgaccatcg acggccgcac ggtgaccgtc aaggggccca agggcatcct
      181 gtcgcacacc gtgatcgagc cgatcacgat cgagcgtgac gagaccggca cggtcgtcgt
      241 caagcgcccg gacgacgagc gccgaaacaa ggccatgcac ggcctgtccc gcacgctcgt
      301 caacaacctc gtcgtcggcg tcacgaacgg ctacgagaag aagatggaga tctacggcgt
      361 cggctaccgc gtggcgctca agggtcggac gctggagttc gcgctcgggt acagccaccc
      421 catcgtgatc gaggcgccgg agggcatcac cttcacggtg gagtcgccca cccggttctc
      481 agtcgccggc atcgacaagc agttggtcgg cgaggtcgcc gccaagattc gcaagctgcg
      541 caagcccgac ccgtacaagg gcaagggcgt gcgctacgcc ggcgaggtca tccgccgcaa
      601 ggtcggaaag acgggtaagt gagcatggcc gagacgattt ccgccaacgc gaggaagcgc
      661 accgcttccc aggtgtcgaa ccagcgccgc accgcgcgca cccgccggca cgcccggctg
      721 cgcaagaagg tcagcggcag cgcagagcgt ccgcgccttg cggtcaaccg gagcgcacgg
      781 cacatcgtcg tgcagctcat cgacgacctg gccggtcaca cgctggcctc ggcgtcgacc
      841 atggaggccg acgtcaggac gctcgagggc gacaagaagg cccgcgcggc caaggtcggc
      901 gagctcgtcg ccgcccgtgc ccgcgaggtc ggggtcaccg cggtggtgtt cgaccggggt
      961 ggctacaact accacggccg gatcgcggcg ctggccgacg ccgcccgcga gggcggcctg
     1021 gagttctgat ggacacgatg cacaaccaga tggaaggaaa cctctgatgc cgggacgcgc
     1081 acggcgtgac ggcgggggcc ccagcaccgg gcccggaggc gagcgcagca acagcggcga
     1141 ccgccgggac cgccgcgacg gaggccgcgg cgggggcggc caggacaaga ccccgtacat
     1201 cgagcgactg gtcacgatca accgcgtggc caaggtcgtc aagggcggtc ggcgcttcag
     1261 cttcaccgcc ctgatgatcg tcggcgacgg cgacggcctg gtgggcgtcg gctacggcaa
     1321 ggccaaggag gtgccggccg cgatcgccaa gggcgtggag gaggcgaaga agagcttctt
     1381 ccgcgttccc cgcatcggcg gcacgatcgt gcaccgtgtg cagggcgagg ccgctgccgg
     1441 tgtcgtgctg ctgcgtccgg cgagtcccgg taccggtgtc atcgctggtg gaccggtgcg
     1501 tgccgtcctg gagtgcgccg gcatccacga cgtcctctcc aagtcgctgg gcagcgacaa
     1561 cgcgatcaac gtcgtgcacg ccacgatcgc cgcgttgaag atgatccagc gtccggagga
     1621 ggtggcggcc cggcgcgggc tgccgctcga ggacgtcgcg ccggcccaga tgctgcgggc
     1681 gcgtgctgcc gcggcgcagg gcgcgaggtc gtgatggccc agacctcgag cgatgccacg
     1741 gcgcagagca ctccgaccag cgacaccaag ctcgtcgtga cccaggtgcg cagcaccatc
     1801 ggcgtcaagg cgaaccagcg tgccacgctg aagtcgctcg gcctgcgcaa gatccgccag
     1861 acggtcgagc gggatgacac cccgcaggtc cgcggcatga tccacaccgt tcgccacctg
     1921 gtgacggtcg aggaggtcgg ctcctgatga gcacgacaat caagattcac cacctgcgtc
     1981 cggcccccgg ggccaagacg gcgaagaccc gggtgggtcg cggcgagggt ggcaagggcg
     2041 gcaagacagc aggccgcggt accaagggca gcaaggcccg caagaacgtg ccggccggct
     2101 tcgagggcgg gcagatgccg ctgcacatgc ggctgcccaa gctcaaggga ttcaagaacc
     2161 ggttccgcgt cgagtaccag gtggtgaacg ttggcgacct cgcccagctg ttccccgcag
     2221 gcggcgcggt cggcccggac gagctggccg aggccggcgc cgtccggcgc aaccagctgg
     2281 tcaaggtgct gggcaacgga gacatcgatg ggatcgcgct caccgtcagc gcgcacgcct
     2341 tctccggtag cgcccgtgac aagatcacgg ccgccggcgg gaccgtcacc gagctgtagt
     2401 tcggtgcccg ttcggtgcgg accggcgtca ttgcgccggt ccgcgccgtg ggggcggcgc
     2461 ggccgctgtt agagtcgtct ccgctcggtc ccacttcagg ccgggcgcgc gccgcattcg
     2521 cggcgcaatc cgtctggggc gggtccgcgc cggagcgggt caccagtaca ggaggatcac
     2581 cgagtgctca gcgcttttcg gtcggcactg accacgccgg atctccggaa gaagatcctc
     2641 ttcacgctcg ggatcctggc cgtctatcgc ctcgggaccg ccatcccgtc gcccggggtc
     2701 tcctatccca acgtgcagga atgcatccgg caggtccaag gcagctcgaa ctccggcatc
     2761 tactcgctga tcaacctgtt ctccggcggg gcgctgctgc agctatcgat cttcgctctc
     2821 ggcatcatgc cgtacatcac ggcgagcatc atcgtgcagc tgctcaccgt tgtcatcccg
     2881 cggttcgagc agctgaagaa ggaagggcag tcgggtcaga acaagctgac ccagtacacc
     2941 cggtacctca cgatcgcgct cgccatcctg caggccacgg ggatcgtggc gctcgccgac
     3001 cggggacagt tgttcggtga ctgctccctg ccggtcatcc ccaactcggg catctaccag
     3061 ctctcggtca tcgtgatcgt gatgacggcg ggcaccgcgg tcgtcatgtg gctgggggag
     3121 caggtcaccg agcggggcgt cggcaacggc atgtcccttc tcatcttcac ctcgatcgcg
     3181 tcccggatcc cggccgaggg cgccaacatc ctgagcaacg gcgccctggt cttcatcggc
     3241 gtctgcgtct tcgggcttgc catcatcgcc agcgtcgtct tcgtcgagca gggccagcgc
     3301 cgcatcccgg tgcagtacgc caagcgcatg gtcggccgtc gcatgtacgg cggcacgtcg
     3361 acgtacctgc cgctgaaggt caaccaggcc ggtgtcatcc cggtgatctt cggatcgtcc
     3421 ctgctctatc tccccgatct cgtctcgcgg ttggccggga gcaccggcgg cgggttccag
     3481 cagttcgtcc agacgtatct ggtggaccag tcgagctggg tgcacatcac cctgtacatc
     3541 gcgctgatca tcttcttcac gtacttctac gtcggcatca cgttcaaccc cgaagagcgg
     3601 gccgacgaga tgaagaagtt cggcggtttc atccccggca tccggcccgg ccggcccacc
     3661 gccgagtacc tcaatttcgt gctctcgcgc atcacgctcc ccggctccct gtacctcggc
     3721 atcatcgccg tgctgccgaa cttcttcctc ggcatcaccg gtagcgggca gaaccagaac
     3781 ttcccgttcg gcggcacggc ggtactgatc atggtcggcg tgggtctcga cacggtcaaa
     3841 cagatcgaaa gccagctcat gcagcgcagg tacgacgggt tcctgcgcta gcgtcgacgg
     3901 atggtctgtc gtgttcgggt tcgataacag gaggtcggtt tcgggtgcgt ctcgttctgg
     3961 ttggcccgcc gggtgcgggc aagggcacgc aggcggtgcg tctggccgag cgtctcgacg
     4021 taccgcacat ctccacgggg gacctcttcc gggcgaattt gaaggacgag acggagctcg
     4081 ggcgcgaggc gaagcggtac atggacgccg gcgacctcgt gcccgacgcg gtcaccgtcg
     4141 cgatggtgcg cgaccgtctc acacacgacg atgccgccaa gggcttcatc ctcgacggct
     4201 tcccgcgcac gttgaggcag gcggcgtcac tcggcgagct gctggccgag cggggcgagg
     4261 acctcgacgc cgtcgtcgag ttccaggtgg cggaggacga gctggtgcgc cggctgatgg
     4321 gccgcggtcg cgaggacgac accgaggacg tcatccgccg ccgccagcag gtctaccgcg
     4381 acgagacggc tccgctcctc gggcactaca gcgaccggct cgtgaccgtc gatgccgtcg
     4441 gtacggtcga ggagatcacc gaccgcgtcg ccaacgcgct ccgctcacgg tgagcggggg
     4501 acgcagcggc gtccgcgggc tgctggcccg ctggcgcggg cgggacatcg agttgaagag
     4561 tccggaagaa ctcgaggcga tgcgggcagc gggcgcgctg gttgccacca ccctcgtggc
     4621 ggtcaccaag ctggcccgac ccggcgtcag cacgggagag ctggacgagc tggccgagca
     4681 ggtcatccgg gaggcgggag cggttccctc cttcctgggc taccacggct acccggcatc
     4741 gatctgcgcg tcggtgaacg agcagatcgt gcacggcatc ccggccgcca cgcaggtgct
     4801 agccgacggc gacctcgtgt cggtggactg tggcgccatc ctcgacggct ggcacggcga
     4861 cgcagcggtg acgatcctgg tcggatcggt gtccgacgcg gaccgcgctc tgtcggcggc
     4921 ctgcgaggcc gcgctctacg ccggtatcgc ggccgtccag ccgggtggcc gcctgtccga
     4981 catctcgcac gccgtgcagg tcgcctcgga gacggccggg aagcgcgacg gtgtcagcta
     5041 cggcatcgtc gccgagtacg gcggccacgg catcggcacg tcaatgcaca tggatccgtt
     5101 cctgccgaac ttcggcgacc cggggcaggg cccgcgactc gtgccgggga tggcgctggc
     5161 cgtggagccg atgctcacgg cgggcgatcc ggagacccgc gagctcgagg atgggtggac
     5221 ggtggtcgcc gccgacggca gccgggccgt gcactgggaa cacacggtcg cgatcacgga
     5281 caacgggccg cgcatcctca cggtcctgcc ggactgaccc gtctgccggc tgcccggccg
     5341 tctgaatcgg tcgagcgtcc cccgcccgta cgtaccccgt cacacgtatc tcctgaatgt
     5401 tcgttccctg ggctacgctc ccgtcttgtg aatacagaat ccacagtacc gcggcgcacg
     5461 accgtggggg tgcctcgtga atccgccgcc ggtgagcgtc gtgtcgccct ggtgccgaag
     5521 gtcgtcgaga aactccggtc gcggggtgtg gaggtcgtgg tcgaggccgg ggccggtctg
     5581 ggcgcgctga ttccggacga gctgtacatc gcggccgggg cgaccgtcgg tgatccgtgg
     5641 tcggccgagg ttgtcgtgaa ggtggcccct ccgtcgccgg aggaggtgga acacctgcgc
     5701 tccggttcca ccctgatcgg tttcctggcg cccctgaccg cacgcgagac gatcgcggca
     5761 ctgaaggccg cgggtgtgca ggcgttcgcg atggaggcga tcccgcggat ctcgcgggcg
     5821 cagtcgatgg acgcgctgtc ctcgcagggg aacgtggcgg gatacaaggc ggtgctgctg
     5881 gccgccgaga agtccacccg gttcttcccg atgctcacca cggcggcggg cacggtgaag
     5941 ccggctactg ccctggtcct gggcgtcggg gtggcgggcc tgcaggccct cgcgacggcc
     6001 aagcggctcg gcgctcggac caccggctac gacgtgcggc cggaggtcgc cgaccaggtg
     6061 cggtcgctcg gggcccagtg gctggacctc ggcatcgaag cggtcggtga gggtgggtac
     6121 gcgcgcgagc tgaccgatga cgaacgggcc gagcagcagc agcgcctgga gaaggcgatc
     6181 accggtttcg atgtggtgat caccaccgcg aacgtccctg ggcggaaggc gcccacgctg
     6241 gtgacggcgg cggccgtgga gggcatgcgt cccggcagcg tcgtcgtgga cctggccggg
     6301 gagagcggcg gcaactgtga gctgaccgag ccgggcgagg tcgtggtcaa gcacgacgtg
     6361 acgatcgcgt cgccgctgaa cctgcccgcg acgatgcccg agcacgcctc ggagctgtac
     6421 tcccgcaacg tatcggcgtt gctggagctg atgctgaagg acgacggcga cggcggggtg
     6481 acgctcgcgc cggactggga cgacgaggtg ctcgcgaagt cctgcgtcac ccgtgtcgag
     6541 gccaccgctg caagtggggg agaggcgtga tggagagcgg actgctgtcg aacatcgcga
     6601 tcctggtgct ggccggcttc gtcggcttcg ccgtgatctc gaaggtgccc aacacgttgc
     6661 acaccccgct gatgtcgggc accaacgcga tccacgggat cgtgctgctg ggtgggttga
     6721 tcgtgctggg ccacctggag aacccatcgg tgatcgacca gatcatcctg gtcatcgcga
     6781 tcgtgttcgg catgatcaac gtgatcggtg gcttcctggt caccgaccgg atgctgggca
     6841 tgttcaaggc caagccggag ccccggcagg cgccgtcggc cgagaaggcc ggcaccaacg
     6901 ggaagggcca ctgatggcga cgctcgtccc gatcctctac atcatcgcgt tcgcgctgtt
     6961 catctacggc ctgatgggcc tgaccggccc gaagacagcg gtgcgcggca acctgatcgc
     7021 cgcggtcggt atgacgatcg ccgtggtggc caccctgctg gaggtccggc acaactgggt
     7081 gctgatcatc gtcgggctcg tgatcggcgc ggcactgggc gtgccgtccg cgcggcaggt
     7141 caagatgacc gcgatgccgc agatggtggc gctgttcaac ggcgtcggcg gcggggcggt
     7201 ggccctgatc gcctggtcgg agttccgcac cagcgatggc ttcctcgcag agcccggata
     7261 cgtggtcgtg gcgaccttgt tcgcggccat cgtcgggtcg atctcgttct gggggtcgct
     7321 gatcgcgttc gggaagctgc aggagatcct gccgggccgc ccgatcggac tgggcaaggc
     7381 gcagcagttc gtcaacgggc tcctgctgct ggccgcggtg gcgtgtgccg tggtggccgg
     7441 gctgggcggt gcgccccaga tctggatcat cgggctgctc gtgctcgcgg cggtgctcgg
     7501 cgtgctggtg gtgctgccga tcggcggcgc ggacatgcca gtggtgatct cgctgctcaa
     7561 cgctctgacg gggctgtcgg ccgcagcggc agggctcgcg ctgaacaaca cggccatgat
     7621 cgtggccggc atgatcgtcg gtgcgtccgg ttcgatcctc accaacctca tggccacggc
     7681 gatgaaccgg tcgatcccgg cgatcgtcgc aggcggcttc ggcggcacca ccacagtgcc
     7741 cggcgcggac gacggcgtcg accgcacggt gaagtcgacc agtgcggccg atgcggcgat
     7801 ccagatggcg tacgcgtcgc aggtgatcgt cgtgcccggc tacgggatgg cggtggccca
     7861 ggcgcagcac gccgtgaagg acatggccaa gctgctggaa gccaagggcg tcgaggtcaa
     7921 gtacgccatc cacccggttg ccgggcggat gcccggacac atgaacgtcc tgctcgccga
     7981 ggccgacgtc tcctacgacg ctctcaagga gatggacgac atcaacgacg agttctcccg
     8041 caccgacgtc acgctggtga tcggcgcgaa cgacgtcacc aatcccgcgg cgcgcaccga
     8101 cccgagctcg ccgatccacg gaatgccgat cctcaacgtc gaccagagtc gctcggtgat
     8161 cgtgctcaag cgctccatga gcagcggttt cgccggcatc gacaacccgc tgttctacgc
     8221 cgacaacacc tcgatgctct tcggcgacgc gaagaagagc gtggccgcgg tgacggagga
     8281 actgaaggcg ctgtaggtcc cgaacgaacg gcacccccgc cggatccctc cggcgggggt
     8341 gccgtgtcgt ttctgtgtca gcagcagcgg ctgccgggtt tcgggtccat cccgtcgatc
     8401 cggcgccgtt cgaactcgcg ccgggagagt ggggcggttc cggcgtgctc ccgggcgtgc
     8461 cgctcgacgt accggtcgta ctgggtctcg ccggtcagct cgcgcaggta ccagagcagg
     8521 cccctgccca gctgcgctat cccggtcacg accgctcgcc cgccgtggcg agtgcccgct
     8581 tctcctcggc cgtcgggatg agccctgacg gtgcgacgat ctgcgacggc acagcaggtg
     8641 cctccgtact gggcagcggc tccgacgagg tcagggctcg gtaccagacg agaagcgcgt
     8701 tggcgatcac gatgatgatc aggatcgcga acagggctgc gagcaccccg tccaccgtcg
     8761 agttggtgac gacggcctgc atgtccgcga tgttcttggc gggtgcgagc acctgcccct
     8821 ggtccagtgc tgcggcgtac ttctcgcgct gcgcgaagaa gccgagcgtc ggatcaggag
     8881 agaacacctt ctggtagctc gccgtgagcg tcaccgcggc gtcccaggcg agcgggacgg
     8941 cggtgaccca ggcgtacttc gcccgcccgg tcttgatcag cagcgtggtg cagaccgtca
     9001 gcgcgacggc ggccagcagc tggttggcga tcccgaacag cgggaagagc tggttgatcc
     9061 cgccgagcgg gtcggtgacc ccggcgtaga ggaagtaacc ccatgcgccc acgacaaccg
     9121 cgctggtgag gtacatgccc ggcttccagt tcacgttgga catcggcttg tacacgttgc
     9181 cgagggtgtc ctggagcatg tagcggccga cccgggtgcc ggcgtccacc gtggtgagga
     9241 tgaacagcgc ctcgaacatg atcgcgaagt ggtaccagaa cgccttgaat gcgtcgccgc
     9301 cgaacgcgcc ggagaagatc tccgacattc cgacggcgag ggtgggcgcg ccacccgtcc
     9361 gagcgatcag cgtgctctcc tgcacggagg cggcggccgc agcgagcgcc tcgggcgtga
     9421 tcgtgaagcc caggttgttc acggcctgcg acgccgactc caccgtcgtg ccgaggagcc
     9481 cggcgggcgc attcatcgcg aagtacaggc cggggtccag cacggatgcc gcgatcagcg
     9541 ccatgatcgc cacgaacgac tccaggagca tcgcgccgta gccgatgaac cggacctgcg
     9601 actccttctg gatcagcttc ggtgtggtgc ccgacgcgat cagagcgtgg aacccggaca
     9661 gcgccccaca ggcgatcgtg atgaacacga acgggaacag cgacccggcg aacacgggcc
     9721 ccttgccgga gaacgcgaac tcggtgaccg gcgcgttctt cagcagcggg gcggtgatca
     9781 gcacgccgac ggccagcaac gcgatcgtgc cgaccttcat gaacgtcgag agatagtcgc
     9841 gtggcgccag cagcatccac accggcagca ccgacgcggc gaagccgtag atgaccaggc
     9901 agaacaccag ggtcgtcggg tcgagggtga aggccgccgc ccagctcgag ttctgcaccc
     9961 agccgcccgc cacgatggcg agcagcagca gcgcgacgcc gatcaggctg gtctcgatga
    10021 cccgcccggg tcggatcacc cgcatgtagt agcccatgaa cagggcgatc gggatcgtca
    10081 tcgcgatgga gaacgtgccc cacggcgact tggcgagcgc attgacgacg accagcgcga
    10141 gcacggcgag caggatgatc atgatggcga acacggcgat cagtgccgcc accccgccga
    10201 tcgggccgat ctcgtcgcgc gccatctgcc cgaggctcct gccgtcgcgg cgcatcgaga
    10261 agaacagagt gaccatgtcc tgcacggcgc cggcgaagat gaccccgacg atgatccaga
    10321 tcgtgccggg caggtacccc atctgcgcag cgagcaccgg cccgaccagc ggaccggccc
    10381 cggcgatcgc cgcgaagtgg tggccgaaca gcacccgacg gtccatgggc tggaagtcgg
    10441 tgccgttgtc cagacgctcc gccggtgtcg cgcggttgtc gtcgacctgc aggaccttct
    10501 tcgcaatgaa ccgcgcgtag aaccggtagg cgatggcgta ggagccgagc gccgccccca
    10561 ccagccagat cgccgagatc tgctcgcccc gggagagggc caacactccc cagcacacgg
    10621 cgccgaccac cgcgaccgcg ccccagatca gcatcgagcg gggcgtcatc cgagatcgca
    10681 gctcggagag cgtcatgcgc cacctccctg ggccgaccca tcacaacggg tgctcctgcg
    10741 ttgcagcatc agttctcctc gcgccctcgc ggcgccgtcg ccgcgttccg atgcactcgt
    10801 tgccgacgta ccccgttcgg ttgacgtccg cgccggagat tctgcgcttt tcgccgccgt
    10861 ttggacacat ctggctgctc ggcgggctcc cgttccgccc gcatgtgtcc agaacgtgat
    10921 gcgagccgcg tcggtgcagg ttccgggcct gcgctcacgc cttctcggcc ggggtgttcg
    10981 actgggtgac gggggcgtcc agtgggtggg cgatctcgtc ggccggcagc cgccacccca
    11041 cgatcgccgc gaccgcaagg aacacgggaa cggctccggc caggacgaac gtcatcggga
    11101 caccgagcca ctcgccgacc ggaccggcga gtgccatcga caccggcatc agcgcgagcg
    11161 acacgaagaa gtccaggctg gagacgcggc ccagcaggtg gggcggtact cggcgctgca
    11221 gcaacgtgcc ccagatgacg gtcgccaccg cgccggtgaa tcccaccacg aacgtcgccg
    11281 cggccatgac ccacaggtgg cgggtgaccc cgatcagcac caacggtgcg gccccggcgc
    11341 cccacagcag gatcatggcc gtcaggtacc gccggggcag tcgccacgag gacacgacga
    11401 tcgacccgac cgcgccaccg atgccgaacg cccccaggac gagagcgaag ctgctcgcgc
    11461 cgccaccgac ctggtcgcgc acggcgaacg gcagcagtac ctcgatcggg ccgatgagga
    11521 cgagcacgta gagcgtggcg aaggcgagtg tggcgaagag ccacccggtg cggaagaggt
    11581 acctgaaccc ctcgaccagg tcggcgagca ccgagttccc ggcttccata ggggtgacgg
    11641 gagctgtgcg catggcgagg agcgcgacga gcgcgagcgc gtagatcccg gccgcgagca
    11701 ggagcgagat cccggggacg aatgcgccga ccagtacgcc ggccagggcc ggaccgaggg
    11761 cctgctgggc gatcggccgc agcgtgccct cgacaccgtt ggcggcgagc agctcgtccg
    11821 gcgggagaag ggtgggcagg atcgccgagt aggcggggta gaagaacgcc tctgccgccc
    11881 cgacgacgaa cgcgatcacg gccaggtgcc agagctgtaa ggcgccggtg accccgagta
    11941 cgcctgcggt ccccgccgag gcgatccgga cggcctccac cgcgatcatg acggcgcgct
    12001 tgggcagccg gtcggcggca acgcccccga tgagcacgct gaccagcagg ccgacgctcg
    12061 acgcggcggc gacgacggac agctcggacg ggccgccgtc gagtgcgatg acctggtaca
    12121 cgatcgcgac gagccacagc ccgctcgcga acagcgacgc cgccatcgac gtggccagca
    12181 agcggtactc gcggtggcga aaggggcgga gtgggcgggg caggcggagt ctgccgtcgg
    12241 cggcctcttc gggcatatcg tccataacgt caccgaacta tgtgaagtca cgtggaggtc
    12301 aaggggcacg gcaccctatc tgcataccgg cggcgtccgc gcccgaattg tccgttggct
    12361 gtgcggagcg gcggggggca ccatggagcg gtggacgacg cggcggagcc cgggcgacct
    12421 ggcgaagtgc ggcccgagga catgcgtgcc ggggacgccg accgcgagcg cgtgctggat
    12481 cggctccgga ccgcccatgt cgagggcagg ctgaccaccg acgagttcga cgagcgaatc
    12541 accgcgacgc tggcggcgcg tacctacggg gagctcggcg cgatcacgat tgacctcccg
    12601 ggcgaggcac cggcgccacc gaccgtggtg gagtccgccg cgccggcggc caccctcgcc
    12661 gagtaccggg acgtcgacgc gctgcaggag cgggacgccc gcggtgccgt gatcgggtgg
    12721 gccggggcga gcgtcacgac gatcgcgatc tgggcaatga gctgcatcgc gacggcctcg
    12781 ttcgtgtacc cctggtggat ctgggtggcc gggccgtggg gcctggtgct gctgtcgtcc
    12841 tgggtgcggg accggcgaca cgagcactga ccagcgcggc ttgtacccca tgtgcgggcc
    12901 cggccgcaac ggtcgtacac tgggagttcg gcgcgcctca tggcgccggt tccgtcgtgc
    12961 ctcccgcacc ccacggtccc ggagtagcgg cgtgcctacg cacggaaccg agagtacgca
    13021 cacctgtcac ggaacagcgg aggacatggc caagaaggac ggggcgatcg aggtcgaggg
    13081 ccgggtcgtc gagcccctgc cgaacgcgat gttccgcgtg gagctggaga acggacaccg
    13141 ggtcctcgcg cacatcagcg gcaagatgcg ccagcactac atccggatcc tccccgagga
    13201 ccgggtcgtg gtcgagctgt cgccgtacga cctgacccgc ggccgcatcg tctaccgcta
    13261 caagtgacca atcgacgaca caacagagag cgatggacgt gaaggtccag ccgagcgtca
    13321 agaagatctg cgacaagtgc aaggtgatcc gccgtcacgg gcgagtcatg gtgatctgcg
    13381 agaacctgcg ccacaagcag cgccagggct gatcgcggct gcgtggtcga cgacgacgca
    13441 gcaccagcac gacgcagcac aagcaacaca gaggaaccca gcgcacctgc cgggccacac
    13501 gggcccggtc cacccccgga accaggccgg ggcccggacg actcgtccgg gacgggcgtt
    13561 gggcagacct ggctcatgag aacagaggag taccccgccg catggcacgt ctctccggcg
    13621 tcgacctccc ccgcgacaag cggatggaga tcgcgctcac ctacatcttc gggatcgggc
    13681 gcacccggtc catccagatc ctcgaggcca ccgggatcga ccgggacctg cggtcgaagg
    13741 acctgaccga cgaggatctc gctgcccttc gcgagtacat cgagaccaac taccgcgtcg
    13801 agggtgatct gcgccgtgag gtgcaggccg acatccgtcg caagatcgag atcggctgct
    13861 accaggggat ccggcaccgc cggggcctgc cggtgcgtgg ccagcgcacc aagaccaacg
    13921 cccgcggccg caagggaccg aagaagacgg tcgccggcaa gaagaaggca ggtaagaagt
    13981 aatgccgccc cgtacccgct ccgcgtcggg accgaagaag gtccggcgca aggagaagaa
    14041 gaacgtcgcg cacggccacg cgcacatcaa gagcaccttc aacaacacga tcgtgtcgat
    14101 caccgacccg accggtgccg tgatcgcgtg ggcctccgcg ggccacgtcg gtttcaaggg
    14161 ctcgcgcaag tccacgccgt tcgccgcgca gatggccgcc gagagcgccg cccgcaaggc
    14221 cgccgagcac ggcatgaaga aggtcgatgt gttcgtgaag ggtcccggtt ccggccggga
    14281 gaccgcgatc cgttccctgc aggccgccgg cctcgaggtc ggcacaattt ccgacgtcac
    14341 cccgcagccg cacaacggct gccgtccgcc caagcggcgc cgggtctagg ggagggagaa
    14401 ccagacatgg ctcgttacac cggacccatg acccgcaagt cccgccggct gaaggtcgac
    14461 ctcgtcggcg gcgaccaggc gttcgagcgc cgtccctacc cgcccggcca gcacggccgg
    14521 atccggatca aggagacgga gtacctcctg cagatgcagg agaagcagaa ggcgcgcttc
    14581 gcatacggcg tgctcgagaa gcagttccgg cgctactacg aagaggcgaa ccggcgcacc
    14641 ggcaagaccg gtgacaacct gctgcagatc ctcgagacgc gtctggacaa cgtcgtctac
    14701 cgggctgggc tcgcccgcac ccggcggatg tcgcggcagc tcgtgaacca cggccacttc
    14761 atggtcaacg gtcacaaggt cgacatcccc agctaccgcg tctcgcccta cgacatcatc
    14821 gatgtccgac cgaagtcgct gggcaccgac ccgttcatca tcgccaagga actggtgggc
    14881 gaccgcccga tccccggctg gctgcaggtc gtcccctcga ccctgcgcat cctggtccac
    14941 cagctgcccg agcgtgcgca gatcgacacg caggtcaccg agcagctgat cgtcgagctc
    15001 tactcgaagt agtccgcgag tagcggctgg acaccccccg gtcccgcagg gaggggccgg
    15061 gcttgctgcg gcatcaaata gcggttgccg caggcctgaa ggaggcacac acccatgctg
    15121 atctctcagc gccccaccct gaccgaggat tcggtcgtcg acacccgctc ccggttcgtc
    15181 atcgagccgc tggagccggg cttcggttac acgctgggca actcgctccg gcgcaccctg
    15241 ctctcctcca tcccgggttc ggcggtgacc agcatccgca tcgacggcgt gctgcacgag
    15301 ttcaccaccg tgcccggcgt gaaggaggac gtcaccgaca tcatcctcaa cctcaaggag
    15361 ctcgtcgtca gctccgagga ggacgagccg gtgacgatgt acctgcgcaa gcagggccct
    15421 ggcccggtca cggcaggcga catcgtgccc ccggccggcg tcaccgtcca caacccggac
    15481 ctgcacatcg ccacgctcaa cgacaagggc cgcctcgagg tggagctcgt cgtcgagcgg
    15541 ggccgcggct acgtcccggc catgcagaac aaggtcaccg gcgccgagat cggccggatt
    15601 ccggtcgact cgatctactc gcccgtgctc aaggtgacct acaaggtcga ggccacccgc
    15661 gtcgagcagc gcaccgactt cgacaagctg gtgctcgacg tcgagaccaa gccctccatc
    15721 accccgcggg acgcgctggc ctcggccggc aagaccctcg tcgagctgtt cgggctcgcg
    15781 cgcgagctca acgtcgacgc cgagggcatc gagatcggcc cgtcgccgca ggaggccgac
    15841 accatcgccg ccttcgcgat gccgatcgag gagctggacc tcaccgtccg ctcctacaac
    15901 tgcctgaagc gtgagggcat ccacaccgtg ggtgagctgg tgggccgcag cgaggccgac
    15961 ctgctcgaca tccgcaactt cggtgcgaag tcgatcgacg aggtcaagat gaagctcgtc
    16021 ggcctcggac tcgcgctgaa ggacagcccg cccggattcg acccgtcggc cgcggccgcc
    16081 gagtacggct ccgacgggtt cgaccccgag accttcgggg acgacggcca ggactacgca
    16141 gaaacggagc agctgtagcc atgcctcagc ccaccaaggg gccccgtctc ggcgggtcgc
    16201 cggcgcacca gcggctgatc ctggccaacc tggccaccgc gctgttcgag cacggccgga
    16261 tcaccacgac cgaggccaag gcgcggcggc tccggccgta cgccgagcgc ctgatcacca
    16321 aggccaagcg cggcgacctg cacaaccgtc gcgagatcca gaaggtgatc cgcgacaaga
    16381 cggtcgtgca ccggctcatc gccgacatcg gtccgttctt cgccgaccgc gagggcggct
    16441 acacccggat caccaagacg ctgccgcgca agggcgacaa cgcccccatg gctgtgatcg
    16501 agctggtcca gcagaagacg gtcaccgacg aggccgagcg cgctcgtcgt gtcgcctcgt
    16561 cgcagcagaa ggccgcggcc gccccggctc ccgccgagac cgagaccgcg gccgccccgg
    16621 ccgaggacac ggcaggcgag gagagcacct ccaccgcgga ggcgccgtac ggcgagggca
    16681 gccacggtcc gctggacgac ccgacggagg ctcccgaggg cttcccgatc aagggcaacg
    16741 aggactcgaa gctgtaccac gtgcccgggt cgtcgcacta cgaccccacc gaggccgagg
    16801 tctggttcgc gtccgcggag gccgccgagg ccgccgggtt cgagctgccc ccgtcgcagc
    16861 gtgaggacga ctccaccgag gagtcctgat agttccgcag cacccgcacg accccgtcga
    16921 gcccgccgtc ctcagcgacg gcgggctcgc gggcgttccg gggcagctcg acctgggaga
    16981 aggggcgact cgggggcggg aaacgggcga ctcgcggagg tttcggctcg atctcgggta
    17041 tgacggcacc gagttctccg ggtgggcgat gcagccgggg cggcggaccg tgtgcggggt
    17101 gctcaccgag gcgctgtcca cggtgctgcg ggagccggtg tcgctgaccg tcgccgggcg
    17161 caccgacgcc ggggtgcacg ccacggggca ggtggcgcac gccgatctcc cggccgagct
    17221 cgacgcgccg tggctggttc gcaggctggc ccggctgctg ccgtccgacg tcagggtgcg
    17281 ggcggtgacg cccgtgcccg cggccttcga cgcgcggttc tccgcactgc gcaggcacta
    17341 ccgctatcgc gtggccaccg ccccgcacgg ggcggaacct ctgcgcgccc gcgacacatt
    17401 gtcctggccg cacccgctgg atctcgcagt ggtggtggca gcgtccgacc gcctcctcgg
    17461 cgagcacgac ttcgccgcgt tctgcaagcg ccgtgaggga gccaccacgg tgcgggcgct
    17521 gcagcggctg tcctgggcgt cggccgacga cggcgtggtg gaagccgccg tctccgccga
    17581 cgccttctgc cactcgatgg tgcggagcct ggtcggggcg ctgctcgacg tcggccgcgg
    17641 ccgacgggcg gtggcgtggc ccgcggagct gctggcacgc cgcgaacgtg cgagcgaggt
    17701 gcccgtcgcg cccgcccacg gtctgactct ggtggccgtg gactacccgc cccccgcgga
    17761 gctcgcggtt cgtgccgccg cgacccgccg cctgcgcgtg cccggcgcgt cgtaggccgt
    17821 ccgttcggcg gcctccccgt gggccctggc gcagttgctg gtcgagcggc ggcgcgctgc
    17881 ggcggggtga acgacgtttc gtcgcgctac gaagtcggtg tggcggcagc gactgttgcg
    17941 gcgcgcccgg ctcgccccgc agagtggggc cgtgaccacg atgttcgatc caccggtgtc
    18001 cgatccggcg gtactgctcg acgtcgcgct cgccgaggcc cgcgcaggcc tcgccgaggg
    18061 cggcatcccc atcggagcgg cgctgttcgc cgccgacggc acgctgctgg gcagcgggca
    18121 caaccgccgt gtgcaggacg acgacccgtc catgcatgcc gagaccgcgg cgttccgggc
    18181 ggccggccga cagcgcgact accgctcgac gatcatggtg acgacgctgt ccccgtgctg
    18241 gtactgctcc ggactggtac gccagttcgg catcggctcg gtgctggtcg gcgaggcccg
    18301 caccttccac ggcgggcacg agtggctcgc cgagcacggc gtcgccgtca cagtgctcga
    18361 cgacgaccgg tgcgcggatc tcatggccga cttcatcgcc gcccgcccgg agctgtggca
    18421 tgaggacatc ggggagccct catgatcccg accatcgacc tggcagcgtg gcgggccggc
    18481 gacgccgcca ctggtcctgc cgtcgactcg gcactgcagc gggccgggtt cctgctggtc
    18541 accgggcacg gcgtggacgc ggcgctgcgc gccgcggtgc gcggcgccgc gcgccggttc
    18601 ttcgcgctgc ccgcggaggt caaggcacgg tacgcggtgc ggatcggcgg ccgtggctgg
    18661 ctcgcccccg gcgtcgaggc caacgggaac gtcgagggca ccgagacgcc gcccgacctc
    18721 aaggagtcct tcgccgtcgg gccggacacg ccgaccggcg acccggaggt ggacggcgtg
    18781 tggttcccgc cgaacgtgtg gcctgccgag gtgccggagc tgcaccacgc cgtcgaccgg
    18841 tatctggcgg cgatgaccgc ggtgtcccgc gacgtgctgg agctgtgcgc cgacgctctc
    18901 cgcctccctc gcgagaccct caccgcgctc gccgcccacc cgacgtggac gttcaacatc
    18961 aaccggtacc caccgctcac cgaggtcggg cagccggcgc cggggcagta ccggatcggc
    19021 ccgcacaccg acttcggcac cgtcaccgtg ctggaccggg agccgggcgc cggcggactg
    19081 caggtcgact gcgccgatgg caacggggac ggcacgggct gggcggatgc cccctacgac
    19141 ccggccgcgc tcaccgtcaa catcggcgac ctgctcgcgc actggactgg cctgcgctgg
    19201 cgttccgggc ggcaccgggt gctgccgccg cagccggact cgccgcacga ggagctcatc
    19261 tcgctggtgt tcttcttcga gctcgaccac gacgcgctgg tcactccgct cgcaccgccg
    19321 atcggccgcc ggacggacct cccgcccgtt gtttccgcgc cgttcctgcg cgagcggctc
    19381 gacgcgatct cagtggggta gggccgcgag tttcggcgaa cgcccgcgag gtggtgatcg
    19441 ccgcggctag cgtcagaccg tgccccgagc gagcgccgtg cgggccccgg ccacccgtga
    19501 ccaggtggac gcataccggt tcggtctgcg ccgcctcgag gcggcgctgg tgcggggcga
    19561 tccggtgccg ctgcacgagc aggtccggtc gcaacgccgc gcggcgctgg ccggggtggt
    19621 gctggggctg ctcgggcttt gcggcgcggc ggctctcgcg aaggtggtgc cggagccgaa
    19681 ctggacgcag caggcggtga tcgtcggcgc ggggtcgggc gcaatgtacg tggtggcgca
    19741 cgggcccgac cggctcgtcc cggtcgccaa cctcccggcc gcccggctcg tgctggcggc
    19801 gctgcgcagt ggcggcagca ccggagccga cccggcaacc gccacccctg tcatggtgcc
    19861 ggacgccgcg ctcgacaccg cggctcgcac accgaccgct gccgtcccgg gcgcggtcgc
    19921 cgtacggccg gacggtcccc ccgtgccgcc gaggtgggcg gtgtgcgacc gaaccacggc
    19981 cgacggcgac ctcgtccgca ccaccgtggt ggcggggatg gcgccgccgg aacccatggc
    20041 gccgggcgac ggggtgctgc tcgacgggcc ggacggtgct acctggctgg tgacggcagg
    20101 ccatcgccac cgcgtcgaca ccggtgacgg caaggtgatg gccgcgttcg ggctcaccgc
    20161 ccgcgttccc cgcgcggccg ctcccgacct ggtgtccctg ctacccgagg ggcctgtgtt
    20221 ggccacgccg gaggtgcccg gccgcggcgg tcccgcccca cgcggggtgc cggggcggat
    20281 cggcgacgtc ctggttgccc atcccgtggg ggccgccgcg cggctcttcg tcgtgttggc
    20341 gggcgggctg caggaggtgc cggacgtggt cgccgagctg ctgcgggtgg cctcgggagc
    20401 atcgcgcgcg ctgcccatcg gtgccgacgt gctggccggc gcggcgttcg tcgaccacgt
    20461 gcgggtggac ggttggccgg ttggtgcgcc acggctccgt gagcccgccg aggcgcccgt
    20521 gacgtgctgg acctggacgg cgaacggtga gccggcaggc gacgtctggg tgggcctggc
    20581 ggagccgctg ccggcgacga ccctgccggt ggccttcgcg caggccgatg ggccggggaa
    20641 ccgggtggac gcggtcgccg tcgggcccgg tggcgccgtg cgtgccaccg ggccgggacg
    20701 cccgcccggc gccggaccgg tgtggctcgt ggcggccacc ggcgtgacct tcggggtggc
    20761 cgacgacgcc acggccgccg ctctcgggat cactgcggca gatcccgcgc cggaggcggc
    20821 gctgcgcctg ctgccgaccg gtccggccct ggacctggcc gctgcgggcc gggtggtgga
    20881 cgtgctgccg ttcggctgac ccctatcggc cctgcgacgc tcagcgcggc aggcgcgagc
    20941 gacggcgcag cagggcggcg gccaccgcgc ccgtcacgag cagcaggccg gccgcgacga
    21001 agtcgatctg cgagctcgac ggggcggcgg ccgggcgcgg tgtggtgccg gccagttcgg
    21061 cgaccgcgac cccggagacc ggcctcgcgg taggttcgag cacggcgggc accgcggtga
    21121 gtgcggcgac cggatcgatc acaccatgac ccagccggtc gtcgcggcca cgggccgggc
    21181 ggcgggccgt gacgaggatg cggtccgcca cctgcttcgc gctgagctcc gggaaccgct
    21241 cacgcaccag tgcggccaga ccggccaccc agggagcggc gaagctcgtg ccgtcgacgc
    21301 cggtggtggt cgtgccgccg ccgacggcca gcgagcgcag ccccgtaccg ggggcggcaa
    21361 cgtcgaccca cggcccgggc accgtgaacg gcgccggagc gtcgtccggg ccgacagcgc
    21421 ccacggcgag cagctcgtcg tcgtaccagc cggggagcga cacctggccg gagcccggct
    21481 cggtgcacga cccggagccc acgttcccgg ccgccaccac gacgacgaca tcggcatcgg
    21541 ccgcgaaccg cagcgcggcg tgcaggccgg caccggccac ggcagcctgg ccggcggcca
    21601 agcagaccgc ctcggagatg ttgatgacgt cggcgccctc ccgcaccgca cgcacgaccg
    21661 cctcggcgag ggtctcggtg tcgcccgccg ggtgggacgt gccgtccggc cccggcacct
    21721 cgaacgaggg gctcgactgg cggatcgcca gcagccgcgc gccgggtgcg atcccgatcg
    21781 gcgcgtcgct ccggccaccg ggccccggcc ccgcggccgc ggcgagcagg cctgcgacgg
    21841 cggtgccgtg cccgtcgcag tcgtcgaggc cgtcgccgcc ggtgaggtag tccccgccac
    21901 cgcgcaatcg gtcgccgagc agccggtggg ggaacacccc ggtgtcgatc acggcgatga
    21961 gcacgcctcg gccggtggcc aggcggtgca cgtcggacag ccgcaggcgg gagcccgtct
    22021 cgtcgtcaac agcctcgtcg tcagcgggcc cgtcgtcgcc cggcagtggg gtacagcgct
    22081 cgggagcgtg cagcccgggc gccggccccg gcggacgtcc tgcgggtggc tcgacgcgga
    22141 cggggggcgg caatggcggc acctcggcgg cggcgggaac tgccgggcag gtgagcgcca
    22201 gcgctgtcgc gaccgcgcag gccgcccgtc tcacaggccg cggaccagtc ggaacaggtc
    22261 catcgccgcg agcgccagcg ggatcgcggc ggcgacgagc acgccctcca gcaggtcgac
    22321 cgcccgccgc gagacgggag agcccatcgg gcgggtacgg ccgaggatga gcgcgccacc
    22381 cgccgccgcg acgagcaggg cgcccgcggc gacgggccgg gccgcggcac ccgcctgcac
    22441 cacgaccagc cccgcgagcg cgacggccgc ggtgatcgcg ctcgccagca gggtgcgggc
    22501 cggtagccgg tcggcgaagc tcctggcccg cagggcgagc accgccacgg tgaccgccgc
    22561 gagggtcggc ccggcccagc cgcctgcgcc ggcgaccggg agcgtggccg cggccgcaac
    22621 cacagcgcag ccgcctgcca gcccggcgag gtagccgcgg gccaggtcgg cccgctccgc
    22681 gagctcggca ggtggcagcc ggtccggccc gttgtcggcg tcgaccagct cgccgccgtc
    22741 ggcgggcacg accggctggg gcagaccggc gagccgcaac gcggcccgcg gcagcaacgg
    22801 gccgatgacg agggcgaccg cgccgaaacc ggccgccacg gcagcggggt cgacgcccaa
    22861 ccggagcacg accatcgtgg ccgcccccac cggcatcgcg gccaccacgg ttccgacgag
    22921 tacgggcgcc acgacgcgga gcgcgatctg ggccaccgcg gcagtgatgc ccgccgctgc
    22981 cgtggccagc agcagttggg ccgcgccgga ggaatgcgac agggctgccc atcccgcggc
    23041 ggccgccagc ggaacggccg caaacgcggc ggtgcgcgcg gacaggtgaa gggaacctcc
    23101 gttgtgctcg tcgctcgtgg cccggcgcgc gagccgggcc gcgtgggcca ccgcgacggc
    23161 cgccgccagc gccgcgacca ggacggttgc gagggtgccg gctgctcccg gcccgcccgc
    23221 gagcaacgcc gccgcggcga cggagacggc cagtaccccg gctgccccga accggtggtc
    23281 ggctgcacca gccgtgcctg ccgtggccgc cagcgcgtcc accgggtcgt cgaacaccgg
    23341 tgggaccggc gccgtaccct ccggcgcgat ccgcagcagc tccccgtcga gcacatcgag
    23401 ctcaccgagg gtggctccgg acggcagcgg cccgcccgcc gcgccggaca gccgccacgg
    23461 ctgcgggcgc agcccgaaga ccggctctcc gaccagctcg agcaccatgg gcaccagctc
    23521 cgcgaccggc acgtcggcgg gtagcgccac gtcgaccctc gcgcgcgggg cgagcaccgt
    23581 gagccggcag tacgtcccgt cggcggcagt ggcgcgcatg gcggagctcc cctctgcgaa
    23641 cgacgggacg acttctccgg aacggccccg ggaggccccg tcggctgcct agcatcgcag
    23701 cgcacgggcc gcgggcaggg ccgttcggcg gaactcgttc gagcggtcgg ctgcacggca
    23761 ggagggggcg ggcatgggca cggtcggagt cgtccggcca cggcgtctcc cgccgcggat
    23821 gcccggcggc gagctcacgc tcgaaccgcc accggagccg gagcgggtcg tccccgcggg
    23881 ggtgctggcc cggctgctgc cggccgtcat gctgctcggt tccgtcggtt tcgtcgccgt
    23941 gctcggcgtg cacaacccca cgtcctggct tttcgggggc atgttcgcca tctcgacgct
    24001 cagcatgatg atgagcggat cgggccgtgg gggcggcacc cggtcggcga gcctcgacga
    24061 ggaccgccgc gactacctgc gctacctgtc gctgctgcgt ggacgcgtcc gcggcatcgc
    24121 cgcggagcag cgcgccgtgc tcgagtcggt gcatccggac ccggcggcat ggcccgcggt
    24181 gctcgcctgc gaccggatct gggagcgcag gcccgccgat gccgacttcg gtcagctgcg
    24241 gatcggccgc ggggcgcagc ggctggccac ccggctggtc gccccgcaga ccgggccggt
    24301 cgagggcctc gagccggtta cggcgctggc gctgcgccgg ttcctgctcg gccacgccgt
    24361 cgtgccggac ctcccggtgg ccctgtcgct gcgcgccagc tccacggtgt ggctggaacc
    24421 ggccgaaccc gcggcggggc tcgggccggc ccgcgggctc gcccgagcgc tcgtcgccca
    24481 gtacgcgctc tggcacagcc cggccgacgc gctgctcgcc gtcgtcgcgc cgccgacgct
    24541 cgctcccgag tgggagtggg tgaagtggct gccgcacctc gcccacccgc gcaggcggga
    24601 cgccgtcggc ccgctccgca tgatcaccga cgacgcggac gacgtgcgtc gctggtgggt
    24661 ggccgagctc gccggcaggc ccgcgggccc gggtatgggt gagccgcacc tgctcgtggt
    24721 cgtcgacgag gtggcgaagg ggccggggcc gtgggcgggt gtcgccgggg tgacggtgct
    24781 gcgggtcggc gcgcccccgg gacggcggcc ggggccgtcc gtcgttcgtc tgatcgtggg
    24841 caccgacggg ctgtaccgca tcggctccgc cgacccggcg cgccactccg agccggcgtc
    24901 agccaacgag cagccgacct ggatcggacg ccccgacggt ctggtcgtcg ccgaggcgag
    24961 ggcgctcgcc cggcgcctgg cgcgctaccg gccagggggc gccgaaccgg cgggcgacac
    25021 gaccccgcgg tccgcgctcg ggctgcccac gctgctcggc ctggagcccg gcccggccgg
    25081 cattgccgcg ctccgcgcac ggtgggcacg tgccgacacc gaccggatgc gcgtgccgat
    25141 cggcgtcgac gagcgcggcg gcccggtcac gctggacctc aaggagtcgg cgcagggcgg
    25201 cagcggcccg cacggcctct gcgtgggcgc caccggctcc ggcaagagcg agctgctgcg
    25261 caccctcgta ctgggccttg ccgccacgca ctcgcccgcc gagctgaacc tcgtgctcgt
    25321 cgacttcaag ggcggggcca cgttcctcgg gctggccggg ctgccgcacg tctcggccgt
    25381 gatcaccaac ctggccgacg agctgacgct ggtggaccgg atggccgacg cgctggccgg
    25441 tgagatcacc cggcgccagg agctgctgcg gtctgccggc aacctcgtgg gtgtcgccga
    25501 ctacgccgca gcccgccgga gtggggccga cctgccgccg ctgcctgcgc tgttcgtcgt
    25561 ggtcgacgag ttctccgagc tgctcgcgca gcgccctgag ctgctcgagc tgctcgtcac
    25621 catcggccgg ctcggacgct cactcgggat ccacctgttg ctcgcctcgc agcgcctcga
    25681 ggagggccgg ttgcgtgggc tggagtcgca cctgtcctac cggatcgcgc tgcgcacgtt
    25741 ctccgcggcg gagtcgcggg cggtgctcgg cgtgcccgac gcccaccagc tgccgtccgc
    25801 ccccggctcg gcgttcctcg cgacgggcac cgacgagctc gtccggttcc gcgccgccta
    25861 cgtgtcggca ccgggcacag caccccctcc gccgggtgtg gcagcgggcc gggcccgcgc
    25921 gtacccgttc cgggcggggc ccgtgccggt ggccgccccg gcagagctcg tcgtgccggc
    25981 ggagcccgac ggcgccgatg gtcccaccgt cctcgacacc gtgatcgacg cgatggcagg
    26041 tcaggggctg cccgcacacc gcgtctggct accgccgctg gatgccccac cacccctcga
    26101 cgaggtgctc ggcccggttc gcccggtgcc gggccgcggg ctcgacgcgg gccggcaagc
    26161 cctgctccgc gttcccgtcg gcctggtcga ccggccctac caacagcgcc gcgaccagct
    26221 cttgatcgac ctggccgggg gatcgggcca cctcgccgtg gtcggcgggc cgcggacggg
    26281 caagtccacg gcgctggcca ccaccgtgct cgggctcgcg ctgacccaca ccccggacga
    26341 gctcggcgtg cacgtgctcg acttcggtgg cggcgcgctc atcccgctcg ccgggctgcc
    26401 gcatgtcggc accgtcgccg accgccagga ggtcgacctc gtgcggcgca ccgtcgccga
    26461 gctctccgct gtcctcgccc ggcgcgagcg cctgttccgc gacgccggga tcgcgtccgt
    26521 cgaggagttc cgggtgcggc gcgccgccgg tgctcatccg ggcgagccga gcaccgacct
    26581 gctgctcgtc gtcgacgggt atctcacgct gcgccgcgag ttcgacgacc tggaggcacg
    26641 gctgctgccg ctggccgcgc agggcctgtc ctacgggctg catctcgcgg tgtccgccac
    26701 ccggtggagc gagctgcgcc ccgccctgaa ggaccttctc ggcggccgcg tcgagctacg
    26761 gctgggcgag ccctcggagt ccgaggtgga tcgccgccgg gccgcggagg tgccggcccg
    26821 gccggggcac gggctcgccc ccgacggtgc cccagctgta ctcgccgcgc cccggctggg
    26881 cgccggtgcc accgaccagg ccgggttggt ggccgcgatc gcagcggcgt ggcccggtcc
    26941 cggcgtcccc ccggtccggc tgctgcccga gcgcatcgat tacaaccggc tgccggctac
    27001 cggcaccggt gccaccggca tcccgatcgg ggtggacgag gagcggctcg accgcgtcga
    27061 gatcgacttc gccgcagaac cccacctgac ctgcttcgcc gacgcggaga gcggaaagac
    27121 gaacatgctg cggctcgtcg cccgcggtgt cacgtcccgg cacgggccgg aggccgcgcg
    27181 ggtggtggtc gtcgaccacc gccggacgct gctgggcgtc gtgccggaca cccatctgat
    27241 cggctacgcc agcaccgtcg acgccaccgc cgaggtggcc cgggaggtgg cggcctcgct
    27301 gcggggcagg ttgcccggcc ctgacgtgac cccgcgccaa ttgcgcgagc gcagttggtg
    27361 gaccggcccc gacgtatacg tcctcgtcga cgactacgac ctcgtcgctc ccggcggggg
    27421 cgcccagcac ccgctcctgc cgctggtggg cttcctgccg caggccaagg atgtgggcct
    27481 gcacctcgtc gtcgcacggc gctgcggtgg agcggggagg gcgctgttcg acccgctcct
    27541 ggggcgcctg cgcgagctcg ccgcaccggg gctggtgatg aacggcagcc cggacgaggg
    27601 cgcgctggtc gagtcggtca aaccggcgcc cctgccgcct gggcgagcca ccctcgtcga
    27661 ccggcgccgc ggggccaggc gaatccagct cgcgtggctt ccgccggacg ccgaggacgg
    27721 cctgtgatcg gccgctcggc ctctgggcgg cgggcgagcg ccggcagccg cccggtgaac
    27781 gtgtgcgtcc cggccggggc gggcggggga ggacggtgag cggcacgccg gttcgcgtcg
    27841 ccgtccagct gggcgcggcc accgcccggc tggcggccgt cggacacgac ggcgagccgt
    27901 ggttggcggc ggaggcgacg gccgccggga ccacggtgcc gtccctgctg gcagagctcg
    27961 tcccctgccc cgaggagctc gtgctcgtcc acccggcggg tcgatccgcg tcgcgtgcgc
    28021 cggcgtggat gggggagggc ctggggctcg ccgggcatgt gcgcgcggtc tcggccccac
    28081 tcgccgcggc cgggcggggt gacgtggtcg tgctcgacgt cgggcacgcg ggggcggagg
    28141 tcacccggct ggatacccac ggccaggtgc tggccctcca ggcccggccg gtgggcggag
    28201 ctgcgctcga cgcgctcctg gtggagctgc tcggactgtc catggccgca cccgccgcgc
    28261 tggccgaggc gcggcgggtg cgcgaggcgc tgtccctgct gccctcggtc gaggtgcgga
    28321 cgcccgagtg cctccgggtg ggcgcgagcg aggtccgcgc ggtactcgcg aggccgctcg
    28381 gtgccgtgat cgaggcgttg cgcgaggtgc tgcggcggac ggggcccgcg ccggtgctgc
    28441 tcgtcggcgg cctggcccgc atgccgctgc tcgcggagtt ggtggacgag gccgggatcg
    28501 ccgacgtgag cgtcgctccc cggccggacg ccgccgccgt gctgggggcg ttgacgcttc
    28561 ccgccaccgc cgggctgacg atcggaccac gggggttcgc ggcgccccgg ccgggtgccg
    28621 caggctcccc ccggagctca ggggcgggat ccgaacggtg tccccgggct gcgcccgcca
    28681 cacccgtacg gcccggtccg gatcgtccgc gtccgatcgg gccgtccgtt gcgggcccgg
    28741 agctcacaac cggaggggac cgcaggcagg tcccggcccg caggctgccc ccactgcctc
    28801 cccgaacccg ccggccgctg ctgcggaccg ctctgctggc cgcagcagcg gtgggtctga
    28861 tcgccggcct cctcggcgtc ggcgccctgt tgccgtcggg gcctgcggcc gccacggtgc
    28921 cggccggggt gctcgtgcag tacggctacc ggctcgacat cccgtcaggg tgggagcaca
    28981 cgggcgggct gccggaacgg cgccggatcc tgctcacccg ggcgggggcg ccggagggca
    29041 gcgacctcat cgcggtcgag cgcaccccgc tcggttacga caccggtgcc gagcccgagc
    29101 gggcgcaggc ggaactgcgg gccgagttcg acgccgcggc gggcgggtcc gcactgtcgg
    29161 gctaccggga cgcccgcatc gcggggcgcg aggccacgag ctaccgacag caggaggcgg
    29221 acgcccggac cgtcgtcgac tggttcgtgc tcctcgacgg cgacgcgcag ctcagcgtgg
    29281 gctgccgata cacggcgacc gggaacgaaa cggtcctcgc cgcgtgcgcc gtcgtcatcg
    29341 cgtcgctgcg gcgcgcctga gggcgagccg ctcgcgcggc ggcctgcctg gtcgacctgc
    29401 gcgacgagac ctcgacggcc cccaccgcgg gccggcgtca gagcctgcgc cgcgagcggc
    29461 gacggcaggc gcgctgtggt ccggctggag ctggtgaacc gatcgggccg cgccttgatc
    29521 acgcccacgg ccaccggtgc ggagcgccga agaccgcggc agccacgccc ccggagtgtc
    29581 gcagggtcgc cgggcccgct cgccggccag gtcgcagcag cccggccagc cgtgccctcc
    29641 ctgcgcgagg cgttgccggc ggatggtcgg cgggcgctcg attccacccc cggggtgccg
    29701 ccccccatcc cgcgttcgca cctgcacacg atcccgcggg ttcgggcccc gcgcggtggc
    29761 gcgccctgcg actgtggccg gacagcagac cgcagcacgg gacaggggtg atcggcatgg
    29821 ccgacgggtt cggtacgacc gtcgaggaga tgcagcgtgc cggacggcac gtgttcgcgg
    29881 tgaacgacac cgtgcaggcc gacctggcca cgttgcgcgg gaggctcgga ccgttggcgg
    29941 gggcgtggcg cggggaggcg tccacggcgt tcaccgggtt gatggcgcgc tgggacgccg
    30001 acgcgcgcgc gctgagcgag gcgttgcgca ccatcggtga ggcgatccag ggcacggggg
    30061 cgagctacca ggcgcaggag gagcagcact ccggcgagct gtccgcgatc cgcgctgcgc
    30121 tgggctgaag ggggagcaat gtctgagatc aaggtcacgt tcggagcgtt ggaggcagcg
    30181 agagcggacg tggggggcac cgccacccgc atctccggcc agttggagga gctcaaacgg
    30241 ttcctcgccc cgatggccgc cacctgggag gggcaggcgg ccgcggaata ccaggtcacg
    30301 cagcgcaggt gggacacggc cgcggccgac ctggctgccg tgctggccca gatcgggctg
    30361 gcgctgggca cggccaacga gagttatcgg caggtggagc aggccaacgc ggcccgctgg
    30421 cggtagccgc cggcggctcc ggaacggggg tgcccggcgg ttggaggggc cgttttgacc
    30481 ccactccgga ccggcgggta cgctcgttct tcgacgtgcg gtgggtggag acctgcggcc
    30541 gcttcaccgc ggcccgcacg gcccgtcacc gggcccggcg ccttccccgc acccgcgcac
    30601 cagaggcagc tcccgagcat ttcgacgagc acacgacagc gacgaggtag gtccgtgccc
    30661 acgtacagcc ccaagcccgg cgagatcacc cgcgcctggc atgtgatcga tgctacggac
    30721 gtggtgctcg gccgcctcgc cacgcacgcc gccacgcttc tgcgtggcaa gcacaagccg
    30781 acgtacgccc cccacatgga cacgggcgac ttcgtcatca tcgtcaatgc ggagaagatc
    30841 gccgtctccg gcaacaagcg ggacggcaag ttcatctacc gccacagcgg ctaccccggc
    30901 ggtctgaggc agcgctcggt cggagagatg atcgagaagc atcccgaccg cctcgtcgag
    30961 aaggcgatca agggcatgct gccgaagaac cgtctcggcc gagcgatgag caagaagctg
    31021 aaggtctacg ccggacccga ccacccgcac gccgcgcagc agccggccgc gttcgagatc
    31081 acccaggtcg cgcagtgacg accgcagaga acgaagaggg acagcccgtg accacccccg
    31141 agcccgggac cgaggccgcc gagacggcag ctgccgagat cgaggccgtc gaggaggccg
    31201 cgctcgacgc cgacgccacg gacctcgccg acgccaccga cgccacggac ctcgccgacg
    31261 ccgccgacga gtacgacgcc gagatcgagg cggtgcctgc catcccggcg gtgttctccg
    31321 accggccgat ccagacggtg ggccgccgca aggaggccgt cgtccgcgtc cggctgatgc
    31381 ccggcagcgg ggcgttccgc ctcaacggca agtcgctcga ggagtacttc ccgaacaagc
    31441 tgcaccagca gctgatccgc gagccgctgg tcaccgtcga gaagaccgag cgcttcgaca
    31501 tcttcgccaa cctgcacggt ggcggcacgt cgggccaggc aggcgcgctg cgcctggcga
    31561 tcgcccgtgc gctgatcgag tccgaccccg aggaccgccc gccgctcaag aaggccggct
    31621 tcctcactcg cgacccgcgc gtgatcgagc gcaagaagta cggcctcaag aaggcccgta
    31681 aggctcctca gtacagcaag cgctgatctt ggctctgtcg gccgggaacc tcgcaagctc
    31741 ggtcaccgtg cgacgtctgt tcggcaccga cggcgttcg
//
LOCUS       DASRSC010000170        15876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4641055,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000170 DASRSC010000000
VERSION     DASRSC010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 15876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15876
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4641055"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1766
                     /gene="recQ"
                     /locus_tag="VFE39_08460"
     CDS             <1..1766
                     /gene="recQ"
                     /locus_tag="VFE39_08460"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HET6258669.1"
                     /translation="AGNDALALMPTGGGKSLCYQIPALVRNGVGVVVSPLIALMQDQV
                     DALRELGVKAAFLNSTLDFAGQRQVEDALRTGNLDLLYIAPERLRVESTLRLLDRGRI
                     ALFAIDEAHCVAQWGHDFRPDYLALSHLHERWPDVPRIALTATATEATHAEIATRLGL
                     DEATARSADPRSADAPRGARHFVASFDRPNIQYRIVPKDEPRRQLLDLLRTEHPGDAG
                     IVYCLSRNSVEKTAEFLVAQGITALPYHAGLDARTRAANQSRFLREDGVIMVATIAFG
                     MGIDKPDVRFVAHLDLPKSVEGYYQETGRAGRDGLPSTAWLAYGLADVVQQRKMIDDS
                     DGDLAHRRRLAQHLDAMLALCETVECRRVQLLNYFGQQGEPCGNCDTCLTPPESWDGT
                     VAAQKALSTVFRLDRERRQKFGAGQIIDILLGKRTAKVEQFDHQSLTVFGVGTELAEG
                     EWRGVIRQLMAQGLLAVEGNYQTFVLTEASRAVLRGERQVMLRRDPQRQARMRSRSPR
                     APQAELPATAAPVFERLRAWRGAAAKEQGVPAYVIFHDATLREIATRMPTTAAELGTI
                     SGIGENKLAKYGEQVLETLAG"
     gene            complement(1792..2643)
                     /locus_tag="VFE39_08465"
     CDS             complement(1792..2643)
                     /locus_tag="VFE39_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6258670.1"
                     /translation="MTAGTGSRVATLPSGEVVAALGQGTWYLGEDPRRRAAEITALRL
                     GLDLGLTVVDTAEMYGDGAAEALVGEAIAGRRDEVFLVDKVLPHHATRAGTVRACQES
                     LLRLGTDRIDLYLLHWRGRVPLAETLAGFADLLDAGAIRYWGVSNFDSDDMAELYGVP
                     GGDAVATDQVLYNLTRRGPEYDLLPWCAEHCVPVMAYSPIEQGRLLGRPALVAVADRH
                     RATPAQVALAWVLRREGTIAIPRSANPEHVRENAAARDIELTADDLAVLDAAFPPPRR
                     PQPLEML"
     gene            complement(2747..5209)
                     /locus_tag="VFE39_08470"
     CDS             complement(2747..5209)
                     /locus_tag="VFE39_08470"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6258671.1"
                     /translation="MVQALPGRARVVIVGGGVGGTSVAYHLAERGERDVLLVERAELT
                     SGSTFHSAGLVGQLRSDPALTRMNGWSVELYRRLQDSEHAPGWVESGSLRLASSPARL
                     EEIRRQAGWAQRSGLPLALISADEARELFPLMSTEGVLGAAYTPTDGQVDPARLCYAL
                     AALARAGGVTIAQQTRVVGIDTMDGRVAGVRTDRGDVECEVVVDCGGMFAAEIARLVG
                     VRIPVVPMSHQYVVTAPLPAGTLPSGRPLPSLRDPDLLVYYRQEVDGLVMGGYERQSA
                     PFTATARSYDAVPQDFNGKLLAPDWDRFDEIVANSQIRVPVLADTGVASMINGPEAFT
                     PDNEFCLGETEVAGFFVAAGFCAHGIAGAGGIGRALAAWILDGDPGLDLWHMDVRRFG
                     RHYRSPGYTLARTVENYESYYDIRYPAAERTSGRPLRTSPAYPWHAANGAVFGEKAGW
                     ERVNHYSTPGSEELRPRGWAGRHWSPCVETEHVAVREAVGLFDETSFSKIEVSGPGAA
                     AFCAHVFAGRTDREPGAVVYTQALNDRGGIEMDVTVTRVASEEFFVVTGTAFGPHDLG
                     WLCRQARLSGAAVRLADVTGAWACFGLWGPRARDVLDPLTPTSLSTLDFPYPAMRETT
                     VGDVPVRAVRVTFVGELGWELYCSTEYGAALWRTLVDAGAPHALRPCGYRAIESLRLE
                     KGYRVWGSDIGPDTTPDEAGLSFAVRRGDGGGGFVGREALLAARARGVVRTLRCLVLG
                     DPRVVALGGEPVRIGAEVVGQVTSGGYGYTVARSIANAYLPSGTAEGTHVAVQVDGTW
                     VTAVVAPSTLYDPKGERIRGEA"
     gene            5262..6215
                     /locus_tag="VFE39_08475"
     CDS             5262..6215
                     /locus_tag="VFE39_08475"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HET6258672.1"
                     /translation="MEVRTAALDPAIDALLDSIPCTARHPRTVEDLPGGLTNRNLKIT
                     NPDGCCVVRIPAEAAGGLLGIDRAHEHANSVAAATAGVGAPVLHHRPGVGLTVGYLPG
                     RPLTDDDLHDPAVLTRVAAACRQLHAGPRFVNDSDMFVVQARYRHIVAEHGFRLPRRY
                     DEFAPQVTLIREALGAGTTVPCNNDLLAANLLDDGRRVRIIDYEYSGNNDPCFELGNI
                     WSEAALPLPLLDVLVTAYYGRRRPGQVARARLFALMAKYGWTLWASIQDGCSPLDFDF
                     WSWGMEKYDRAVREFDAPDLEHLLRAARRPDIERQLDAEAP"
     gene            6244..7587
                     /locus_tag="VFE39_08480"
     CDS             6244..7587
                     /locus_tag="VFE39_08480"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET6258673.1"
                     /translation="MLTIDQLRAAVDDGSIDTVLVAFTDMQGRLQGKRCAAPYFLEEV
                     VAHAAEACNYLLAVDVEMNTVGGYAMSSWERGYGDFVLRPDMATLRRVPWHEATALVL
                     CDAQWADGSPVVASPRQVLRGQLDRLAEHGLAADVGTELEFMLFADSFDTAWRKAYHD
                     LEPANLYNVDYSMLGTARVEPLLRRIRNGMAGAGMVVESAKGECNLGQHEIAFRYADA
                     LTTCDNHSIYKTGAKEIADQSGMALTFMAKYDEREGNSCHIHISLRGKDGAVFPGDGT
                     HGFSPLFAHFLAGQQACLQELTYLFAPNVNSYKRYAEGSFAPTAVAWGLDNRTCALRV
                     VGHGPSLRVENRVPGGDMNPYLGVAALIAAGLHGIENELPLEPELRGNAYTSDAPRVP
                     VTLREAAELFAESKVAVDAFGQEVVDHYANAARVELAAFDATVTDWERRRGFERL"
     gene            7568..8299
                     /locus_tag="VFE39_08485"
     CDS             7568..8299
                     /locus_tag="VFE39_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET6258674.1"
                     /translation="MASNGSSSPVVGLTAYGEHVAYGVWDHHAVLLPRTYPDVVLAAG
                     GVPVLLPPRVDAATAVDRLDALVLSGGPDIEPQRYGATPHPGTGVARPERDAAEIAML
                     HRALERGIPVLGVCRGVQMINVALGGTLVQHLPDAVGHQGHNPSPGVFGTTVVALVPG
                     SRVATAVGTAVAVQCHHHQALDRIAPGLTVTGRAADGTVEAAELAGAPFVVGVQWHPE
                     QDATDVRLMAALVAAAARTRAVRTA"
     gene            8359..9405
                     /locus_tag="VFE39_08490"
     CDS             8359..9405
                     /locus_tag="VFE39_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258675.1"
                     /translation="MTSRRLHLIGSYPADTTDDAMNEIMELAGPYLTSLPDGETGDRR
                     NWIVPIIESMRLHPDLEVRREGDWSGYDHLLSFRVRRGHRLAGDSLHFGYTEAHAAAR
                     PVFERVREAHGRSDLAFQIGMPSGLGMAMFTMGPPGPLVHRRPFTDAIAREITQIRAD
                     AADDVIVQLELPAELAFVTMTPGPLRVAVAAWLSRTIASLVRATPGGTRFGLHLCMGD
                     QRHEAMFRMRDTRPVVALLNAVGARWPAGYSLEFVHAPLAAGAAPPSLDPAFYAALAA
                     LRLPAHTRFVAGLLHEDRSADELLGVVSAVERALGRTVDLGASCGLGRRSREMAQSIL
                     QQGAQLCGPVVGAP"
     gene            9457..10224
                     /locus_tag="VFE39_08495"
     CDS             9457..10224
                     /locus_tag="VFE39_08495"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET6258676.1"
                     /translation="MIDRFAGRTAVVTGGGSGIGLATTRRLAEEGARVVVADIDAGPG
                     QAAADEVGGLFVRTDVTSEVEVEALFAAAAEHYGSVDVAFNNAGISPPEDDSILTTGL
                     DAWRRVQEVNLTSVYLCCRAAIPHMQRAGRGSIVNTASFVAVMGAATSQISYTASKGG
                     VLAMSRELGVQFARENIRVNALCPGPVDTPLLQELFAADPERAQRRLVHIPMGRFARP
                     EEIAGAVAFLASDDASFITASTFLVDGGISGAYVTPL"
     gene            complement(10295..10525)
                     /locus_tag="VFE39_08500"
     CDS             complement(10295..10525)
                     /locus_tag="VFE39_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258677.1"
                     /translation="MGEAIAGRRDEVFLVSKVLPHHATRRGDGAGLRGQPAPPGSDRL
                     DLYLLHWRGAIPLEETLAGFSDLMAAGSASGA"
     gene            complement(10816..13056)
                     /locus_tag="VFE39_08505"
     CDS             complement(10816..13056)
                     /locus_tag="VFE39_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HET6258678.1"
                     /translation="MLYERLDARRADTAQRLHSVLHDETVGTPQALTERDAAAAMYTD
                     RLAALRAAEHGLAFGRLDTDDGERRYVGRLGLLDEENEYEPLLMDWRAPAARPFYTAT
                     AASPEGIRRRRHLRTRRREVTAVDDEVLDLDDATAVSQAGGLTSEAALLAAVDSRRTG
                     RMSDIVATIQAEQDTIIRSKPSGVLVVQGGPGTGKTAVALHRAAYLLYTHRDRLARRG
                     VLVVGPNPTFLSYIGQVLPSLGETSVVLGTVGQLHPGLDARRAEPAATAAVKGRAEMA
                     PVIAAAVRDRQQLPRRPIELVVEQQRVRLDRDIANAARTRARRSRKPHNEAKRTFHKE
                     AVRLLAEQVARTLGGRGLLDAGDLADIREELTESRALTRELDTLWPTLSAEQLLTDLF
                     ASPRRLNTVARRLPAADRELLQRPAPAADVPPNQRWTPADVPLLDEAAELLGDDGAEA
                     AAREAAALREEVMYAQGVLDVLDLEEDLDPELLRATDVVDADRLAERQAVRRYDSTAD
                     RAAADREWTYGHVIVDEAQELTPMAWRLIMRRCPSRSMTIVGDIAQTGDLSGATSWGE
                     VLSPFVANRWRLEQLTVNYRTPSEIADVADDVLAAIEPGLTAPRSVRSTGVPPRAVGV
                     DGSRLDDAVAKVLAEEAGAVGDGRTAVLAPTARVAALRERLVGEPPADAPDIDLSAPV
                     VVLPVNSAKGLEFDAVVVVEPAELLAESSRGANDLYVALTRSTQRLAVVHSQPLPAML
                     HRLHHA"
     gene            13329..14099
                     /locus_tag="VFE39_08510"
     CDS             13329..14099
                     /locus_tag="VFE39_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigF"
                     /protein_id="HET6258679.1"
                     /translation="MSESSRDSDYAHLAPLFAEFASLPPDDPGRVALRGKLVTGYLPV
                     AQHIARRFAGRGEPVDDLEQAGTVGLLNAVDRFEPDRGIDFLSYAVPTITGEIRRHFR
                     DRTWSMRVPRRLKDLQGAINGAIGPLSQELGRAPRPSEIAARLGIPTEEVVEGLDAQQ
                     AYRSTSLDELVAGADTTLTDTLGSADTELEKVEYRETLAPLLDELPERERTILLLRFF
                     GNLTQTQIADHVGISQMHVSRLLSQTVAQLRRRMTDDG"
     gene            14128..14616
                     /locus_tag="VFE39_08515"
     CDS             14128..14616
                     /locus_tag="VFE39_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6258680.1"
                     /translation="MTTNVLHSIDVAVPVSTAYNQWTQFESFPRFMDGVERIEQVTPT
                     RTHWVTKIAGVEREFDAEITEQNPDERVAWTTEKGTHQAGVVTFHRIDAAHTRVTLQL
                     DHDPQGLVEKAGDALGIVQRRVKGDLQNFKEFIETRGSEDGGWRGDVPRSPQAGDTTP
                     PA"
     gene            complement(14638..15183)
                     /locus_tag="VFE39_08520"
     CDS             complement(14638..15183)
                     /locus_tag="VFE39_08520"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HET6258681.1"
                     /translation="MEGPDTGASSAGSERPGEGPLQRADRNMLELLQELRVAQTGVQI
                     LFAFLLSLSFTDRFAEIDAVQRWTYVVTLLLSVVTAGLLVAPAAVHRVTFRRGVKQET
                     VEMGHRLFTVGLATLALTLTGAVLLVLDVAVNRAFAISVAAAVGVVLLAMWFALPLRL
                     LRRSPANAERPPPGDRGAARL"
     gene            complement(15223..15804)
                     /locus_tag="VFE39_08525"
     CDS             complement(15223..15804)
                     /locus_tag="VFE39_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HET6258682.1"
                     /translation="MAGKLAGKRIAILATDGVEQVELTRPRDAVTQEGARTEIVSLSE
                     GEIQAMNNDIEPADTFTADRSAKEASAADYDALIMPGGTVNADRLRMSPDVVAFVQEI
                     FRSGKPVGVICHGPWTLVEADLVRGRTLTSYPSLRTDIRNAGGTVLDEEVVTDHGLVS
                     SRNPDDLPAFCAKLVEEFTEGAHQVHEEGATVS"
BASE COUNT         2281 a         5794 c         5588 g         2213 t
ORIGIN      
        1 tcgccggcaa cgacgctctg gcgctgatgc cgacgggtgg cggaaagtca ctctgctatc
       61 agatcccggc gcttgtccga aacggcgtcg gcgtcgtcgt gtcacccctc atcgcgctga
      121 tgcaggacca ggtcgacgcc ctgcgcgagt tgggcgtcaa ggccgcgttc ctcaattcga
      181 cgctcgattt cgccggccag cggcaggtcg aggacgcgct ccggaccggc aacctcgacc
      241 tcctctacat cgccccggag cggctgcgcg tggagtccac cctgcgcctg ctcgaccgcg
      301 gcaggatcgc gctgttcgcc atcgacgagg cgcattgcgt cgcgcagtgg ggccacgact
      361 tccgccccga ctacctcgcc ctctcgcacc tgcacgaacg ctggccggac gtgccccgca
      421 tcgcgctcac cgccaccgcc accgaggcca cccacgcgga gatcgccacc cggctgggcc
      481 tcgacgaggc cactgcgcgc tccgccgatc cacgctccgc tgacgctccg cgaggtgccc
      541 ggcacttcgt cgcgagcttc gatcggccca acatccagta ccggatcgtg ccgaaggacg
      601 aaccccgccg ccagctgctc gacctgctgc gcaccgagca tcccggcgac gcgggcatcg
      661 tctactgcct ctcgcgcaac tcggtggaga agacggccga gttcctcgtg gcgcagggga
      721 tcaccgcgct gccctaccac gccggcctcg acgccaggac gcgcgcggcg aaccagtcgc
      781 ggttcctgcg cgaggacggc gtgatcatgg tggccacgat cgcgttcggc atgggcatcg
      841 acaagcccga cgtccggttc gtcgcacacc tggacctgcc gaagtccgtg gagggctact
      901 accaggagac cggccgggcc gggcgcgacg ggctaccgtc cacggcgtgg ctcgcctacg
      961 ggctcgccga cgtcgtgcag cagcgcaaga tgatcgacga ctccgacggc gacctcgccc
     1021 accgccgcag gctcgcccag cacctcgacg cgatgctcgc gctctgcgag acggtcgagt
     1081 gccggcgggt gcagctgctg aactacttcg ggcagcaggg cgagccgtgc ggcaactgcg
     1141 acacgtgcct gacgcccccc gagtcctggg acggcacggt ggccgcgcag aaggcgctct
     1201 ccacggtgtt ccggctcgac cgggagcggc gccagaagtt cggggccggg cagatcatcg
     1261 acatcctgct cgggaagcgc accgcgaagg tcgagcagtt cgaccaccag tcgctcaccg
     1321 tgttcggtgt gggcaccgag ctcgccgagg gcgagtggcg cggggtgatc cggcagctga
     1381 tggcgcaggg cctgctggcc gtcgagggca actaccagac gttcgtcctc accgaggcga
     1441 gtcgcgctgt gctgcgcggc gagcggcagg tgatgctgcg gcgcgatccg caacggcagg
     1501 cacggatgcg ttcccgctcg ccgcgcgcac cccaggcgga gctgcccgcc acagcggcgc
     1561 cggtgttcga gcgactgcgg gcgtggcgcg gggcggcggc gaaggagcag ggcgtgccag
     1621 cctatgtgat cttccacgac gccacgctgc gcgagatcgc cacccggatg cccacaaccg
     1681 cggccgagct gggcacgatc agcggcatcg gggagaacaa gctcgcgaaa tacggcgagc
     1741 aggttctcga aactctcgcc ggctgaggcg aaccggggtg ctgcgcccgc gtcagagcat
     1801 ctcgagcggc tgcggacggc gcgggggcgg gaaagctgcg tcgagcacgg cgaggtcgtc
     1861 tgcggtgagc tcgatgtcgc gggcggccgc gttctcccgg acgtgctcgg ggttggcgga
     1921 gcgcgggatc gcgatcgtgc cctcccgccg cagcacccag gccagcgcca cctgggcggg
     1981 cgtcgcgcgg tgccggtcgg cgaccgcgac cagtgcgggc cggccgagca gcctgccctg
     2041 ctcgatcggc gagtaggcca tcaccgggac gcagtgctcg gcgcaccacg gcagcaggtc
     2101 gtactccggg ccacgccgtg tgaggttgta aagcacctgg tcggtcgcca cggcatcgcc
     2161 gcccgggacg ccgtacagct cggccatgtc gtcgctgtcg aagttgctca ccccccaata
     2221 ccggatcgca cctgcgtcga ggaggtcggc gaatccggcg agggtctcgg ccaggggcac
     2281 tcgcccgcgc cagtgcagca ggtacaggtc gatgcggtcg gtgccgagcc ggagcaggct
     2341 ctcttggcac gcccgcaccg tgccggcgcg ggtggcgtgg tggggcagca ccttgtcgac
     2401 gaggaacacc tcgtcgcggc ggccggcgat ggcctccccg accagcgcct ccgcggcccc
     2461 gtcgccgtac atctcggcgg tgtcgaccac ggtcaggccg aggtcgaggc ccagccgcag
     2521 cgccgtgatc tcggcggcgc gccgccgcgg atcctccccc aggtaccacg tgccctgccc
     2581 gagagccgcc accacctcac ccgatggcaa ggtcgcgacg cggcttccgg ttccggctgt
     2641 catccgcacc tcctggtctc ggtggtcacc gcaggccgca caccaccgtc ccacccaggg
     2701 cgtccacgtg cggctcgccc ggaccagtgc ggcgatcgtg tggtgatcag gcttctccgc
     2761 ggatccgttc gcccttcggg tcgtacaagg tcgacggcgc cacgacggcg gttacccagg
     2821 tgccgtcgac ctgtaccgcc acgtgcgtgc cctcggcggt gcccgagggc aggtaggcgt
     2881 tggcgatcga gcgggcgacc gtgtagccgt agccgccgga ggtcacctga ccgacgacct
     2941 cggccccgat ccgcacgggc tcgccaccca gcgcgaccac acgcggatcc ccgagcacga
     3001 ggcaccgcag cgtccggacg acccctctcg cccgggcagc cagcagtgcc tcgcgcccga
     3061 cgaacccgcc gccaccgtcc ccccggcgga cggcgaagct cagaccggcc tcgtcggggg
     3121 tcgtgtcggg gccgatgtcg gagccccaga cccgataccc cttctccagc cgcaggctct
     3181 cgatcgcgcg gtacccgcac ggtcggagtg catgcggcgc accggcgtcg acaagggtgc
     3241 gccagagcgc agcgccgtac tccgtggagc agtacagctc ccaccccagc tcccccacga
     3301 acgtgacgcg caccgcccgc accgggacgt ccccgacggt cgtctcccgc atcgccggat
     3361 agggaaagtc tagagtggac agtgaggtcg gggtgagcgg gtcgagcacg tcccgggcgc
     3421 gcggccccca cagcccgaag cacgcccatg cccccgtcac gtcggccagc cgcaccgctg
     3481 cacccgacag tcgagcctgc cgacacaacc agccgaggtc gtgcggcccg aacgccgtgc
     3541 ccgtcaccac gaagaactcc tcggacgcga cgcgggtgac ggtgacgtcc atctcgatcc
     3601 cgccacggtc gttgagcgcc tgcgtgtaga cgaccgctcc gggctcacgg tcggtccgcc
     3661 cggcgaagac gtgggcacag aatgcggcgg cgcccggtcc ggacacctcg atcttcgaga
     3721 aggaggtctc gtcgaacagg cccacggcct ctcgcacggc gacgtgctcg gtctccacgc
     3781 agggcgacca gtgccggcct gcccagccgc gcggccgcag ctcctccgac ccgggagtgg
     3841 agtagtggtt cacccgctcc caaccggcct tctccccgaa caccgccccg ttggccgcgt
     3901 gccacgggta ggcgggcgag gtgcgcagcg gccggccgga cgtgcgctcg gcggccgggt
     3961 agcggatgtc gtagtagctc tcgtagttct ccacagtgcg ggcgagcgtg tagcccggcg
     4021 agcggtagtg ccggccgaag cgacgcacgt ccatgtgcca caggtcgagg ccggggtcgc
     4081 cgtcgaggat ccacgcggcc agcgcgcgcc cgataccccc ggcgccggcg atcccgtgcg
     4141 cgcagaagcc ggccgccacg aagaaccccg cgacctcggt ctccccgagg cagaactcgt
     4201 tgtccggggt gaatgcctcc ggaccgttga tcatcgacgc cacgccggtg tcggccagta
     4261 ccggaacccg gatctgcgag ttggcgacga tctcgtcgaa ccggtcccag tccggcgcga
     4321 ggagcttgcc gttgaagtcc tggggcacgg cgtcgtagga gcgggcggtg gccgtgaagg
     4381 gcgcgctctg ccgctcgtag ccgcccatca ccagcccgtc gacctcctgc cggtagtaga
     4441 cgagcaggtc ggggtcgcgc agcgacggca gcggcctccc ggacgggagc gtgccggccg
     4501 ggagcggtgc ggtcaccacg tactggtgcg acatcggcac cacggggatc cggacgccca
     4561 cgagccgggc gatctccgcg gcgaacatgc ctccgcagtc gacgaccacc tcgcactcga
     4621 cgtccccacg gtcggttcgg acgccggcga cccgtccgtc catggtgtcg atcccgacca
     4681 cccgcgtctg ctgcgcgatc gtcacgccgc ccgcgcgggc gagcgcggcg agggcgtagc
     4741 agagccgcgc cggatcgacc tgcccgtcgg tcggggtgta cgcggccccg agcaccccct
     4801 cggtggacat cagtgggaag agctcgcgcg cctcgtcggc ggagatcagc gccagcggca
     4861 ggccggaccg ctgtgcccag ccggcctgcc ggcggatctc ctcgaggcgg gccggtgagg
     4921 acgccagccg cagtgagccg gactcgaccc agccgggcgc gtgctcgctg tcctgcagcc
     4981 tgcggtagag ctcgaccgac cagccgttca tcctggtcag cgcggggtca gagcgcagct
     5041 ggccgaccag gcccgccgag tggaacgtgg agccgctggt gagctcggcg cgctcgacca
     5101 gcagcacgtc acgctcaccc cgctccgcga ggtggtaggc cacgctcgtc ccgcccactc
     5161 ccccaccgac gatcacgacg cgggcccgcc cggggagcgc ttgcaccatg tcgcgacact
     5221 acggtctaga ccgttcatgg cgcgagccct accatccagc gatggaggtg cggaccgcgg
     5281 cactcgatcc cgcgatcgac gccctgctcg actccatccc ctgcaccgcc cggcaccccc
     5341 gcacggtcga ggacctgccc ggcgggctca ccaaccgcaa cctcaagatc accaaccccg
     5401 acgggtgctg cgtggttcgg attcccgccg aggcggctgg cggcctgctc ggcatcgacc
     5461 gcgcacacga gcacgccaac tccgtggccg cagccaccgc gggcgtcggc gcaccggtgc
     5521 tgcaccaccg gcccggcgtc ggactgaccg tgggctacct gcccggtcgg cctctcaccg
     5581 acgacgacct gcacgacccg gccgtgctga cccgcgtggc ggccgcctgc cggcagctgc
     5641 acgccgggcc gcggttcgtc aacgactccg acatgttcgt ggtgcaggcg cgctaccggc
     5701 acatcgtcgc cgagcacggc ttccggctgc cgcggcgcta cgacgagttc gccccgcagg
     5761 tgacgctgat ccgggaggcg ctcggggccg gcacgacggt gccgtgcaac aacgacctgc
     5821 tcgccgccaa cctcctggac gacgggcggc gggtgcggat catcgactac gagtactcgg
     5881 gcaacaacga cccctgcttc gagctgggca acatctggag cgaggccgcg ctgccgctgc
     5941 cgctcctcga cgtgctcgtc accgcgtact acggacggcg ccgcccaggg caggtcgcca
     6001 gggcgcggct gttcgcgctc atggcgaagt acggctggac gctgtgggcg tcgatccagg
     6061 acggctgcag cccgctggac ttcgacttct ggtcgtgggg gatggagaag tacgaccgcg
     6121 ccgtccggga gttcgacgct cccgacctcg agcacctgct cagggcagca cgacgacccg
     6181 acatcgagcg gcagctggac gccgaagccc cgtgagatca tcctccgcat gagacgacac
     6241 gggatgctca cgatcgacca gctgcgcgca gccgtggacg acgggtcgat cgacaccgtg
     6301 ctcgtcgcgt tcaccgacat gcagggccgc ctgcagggca agcggtgtgc ggccccgtac
     6361 ttcctcgagg aggtcgtggc gcacgcggcc gaggcctgca actacctgct ggccgtcgac
     6421 gtggagatga acaccgtcgg cggctacgcg atgtcgagct gggagcgtgg ctacggcgac
     6481 ttcgtcctga gaccggacat ggcgacactg cgccgcgtcc cgtggcacga ggccaccgcc
     6541 ctcgtgctgt gcgacgcgca gtgggcggac ggctcgcccg tcgtcgcgtc gcccaggcag
     6601 gtcctgcgcg gccagctcga ccggctcgcc gagcacgggc tcgcggccga cgtgggcacg
     6661 gagctggagt tcatgctctt cgccgactcg ttcgacaccg cgtggcgcaa ggcctaccac
     6721 gacctcgagc ccgcgaacct ctacaacgtg gactactcga tgctcggcac cgcgcgcgtg
     6781 gaaccgttgc tgcggcggat ccgcaacggg atggccggag cgggcatggt cgtcgagtcg
     6841 gcgaagggcg agtgcaacct cggccagcac gagatcgcgt tccgctatgc cgacgcactc
     6901 accacctgcg acaaccactc catctacaag accggggcca aggagatcgc cgaccagtcg
     6961 ggcatggcgc tcaccttcat ggccaagtac gacgagcgcg agggcaactc ctgccacatc
     7021 cacatctcgc tgcgcgggaa ggacggagcg gtcttccccg gggacggcac gcacggcttc
     7081 tccccgctgt tcgcgcactt cctggcaggt cagcaggcat gcctgcaaga gctcacctac
     7141 ctgttcgcac cgaacgtcaa ctcctacaag cgctacgccg agggctcctt cgcgcccacg
     7201 gccgtcgcct ggggcctgga caaccgcacc tgcgcgctgc gcgtcgtcgg gcacggcccg
     7261 tcgctacggg tggagaaccg ggtgcctggc ggtgatatga acccttacct gggggtcgcg
     7321 gcgctgatcg cggcagggct gcacggcatc gagaacgagc tcccgctcga gccggagctc
     7381 aggggcaacg cctacacgtc cgacgcaccg cgggtgccgg tcacgctgcg ggaggcggcc
     7441 gagctcttcg ccgaatcgaa ggtggccgtc gacgcgttcg gccaggaggt cgtggaccac
     7501 tacgccaacg cggcgcgggt ggagctggcc gccttcgacg cgacggtcac cgactgggag
     7561 cgcaggcgtg gcttcgaacg gctctagcag cccggtcgtc gggctcacgg cctacggcga
     7621 gcacgtcgcg tacggggtgt gggaccacca cgcggtgctg ctgcctcgga cctatcccga
     7681 cgtcgtgctc gccgccggcg gggtccccgt gctcctaccg ccccgcgtcg acgccgcaac
     7741 cgccgtcgac cggctcgacg cgctcgtgct gtccggcggc cccgatatcg agccgcagcg
     7801 gtacggcgcc accccacacc cgggtaccgg tgtcgcgcgt cccgagcgcg acgcggccga
     7861 gatcgcgatg ctgcaccggg cgctcgagcg cggcatcccg gtactgggtg tgtgccgggg
     7921 cgtacagatg atcaacgtcg cactcggtgg caccctcgtc cagcaccttc ccgacgccgt
     7981 cgggcaccag ggacacaacc cctccccggg cgtcttcggc accaccgtgg ttgccctggt
     8041 cccgggcagc cgcgtggcca ccgccgtcgg caccgcggtg gccgtgcagt gccaccacca
     8101 ccaggcgctc gaccgcatcg cccccgggct gaccgtcacc gggcgcgccg cggacggcac
     8161 ggtggaggcc gccgagctgg cgggggcacc cttcgtggtg ggagtccagt ggcatcccga
     8221 gcaggacgcc accgacgtgc gcctgatggc cgcgctggtc gcggcggcgg cccggacccg
     8281 agcggtgcgg acggcatgag tcgccggctg tcgtgatcag ctcttgacga tctcttggtg
     8341 attgctcacc ctcggtgagt gacgagtcgc cggctgcacc tgatcggaag ctatccggcc
     8401 gacaccaccg acgatgcgat gaacgagatc atggagctgg cgggcccgta cctgacctcg
     8461 ctccccgacg gcgagaccgg cgaccggcgc aactggatcg tccccattat cgagagcatg
     8521 cgcctgcatc ccgatctgga ggtgcgtcgc gagggcgact ggtcgggcta cgaccacctc
     8581 ctcagctttc gggtgcgccg cggccatcgg ctcgccggtg actccctcca tttcggctac
     8641 accgaggccc acgccgccgc ccgccccgtc ttcgagcggg tgcgcgaggc gcacggtcgc
     8701 agcgatctcg cgttccagat cggcatgccg agcggcctcg gaatggcgat gttcacgatg
     8761 ggtccgccgg gaccactcgt gcaccggcgc cccttcaccg atgccatcgc gcgggagatc
     8821 acgcagatcc gcgccgacgc ggccgacgat gtgatcgtcc agctcgagct gcccgccgag
     8881 ctggccttcg tcacgatgac cccagggccg ctgcgcgtcg cggtggccgc gtggctcagc
     8941 cgcaccatcg cctcgctggt gcgcgccacc ccgggcggca cgcgcttcgg actgcacctg
     9001 tgcatgggtg accaacgcca cgaggccatg ttccggatgc gcgacacgcg accggttgtg
     9061 gcgctgctca acgccgtcgg cgcacgctgg cccgccggct actccctcga gttcgtccac
     9121 gccccgctgg cggcgggcgc cgcaccgccg tcgctggacc cggcgttcta cgcggcgctg
     9181 gcggcgctgc gcctcccggc tcacacccga ttcgtcgccg ggctcctgca cgaggaccgg
     9241 tcggcggacg agctgctcgg ggtcgtgtcg gccgtggaga gggccctcgg ccgcacggtc
     9301 gacctcgggg cgtcgtgcgg tctggggcgg cggtcccgcg agatggcgca gtcgatcctg
     9361 cagcagggcg cgcagctctg cgggcccgtg gtgggcgcgc cgtagggagg gcggtccgcg
     9421 gcagggcgcg ccgcgccgcg gcattagtct cgcggcatga tcgatcggtt cgcgggacgc
     9481 acggcggtcg tgacgggcgg cggcagcggc atcgggctgg ccacgacccg ccggctcgcc
     9541 gaggagggcg cacgcgtcgt cgtcgccgac atcgacgccg ggccgggaca ggcggcggcc
     9601 gacgaggtgg gcgggctctt cgtgcgcacc gacgtgacgt ccgaggtcga ggtcgaggcg
     9661 ctgttcgcag cggcggcgga gcactacggc agcgtggacg tcgcgttcaa caacgcgggc
     9721 atctccccgc cggaggacga ctcgatcctg acgacgggcc tcgatgcgtg gcggcgcgtg
     9781 caggaggtga acctcacctc ggtgtatctg tgctgccggg ccgcgatccc gcacatgcag
     9841 cgggccggtc gcgggtcgat cgtcaacacg gcgtcgttcg tggcggtcat gggtgcggcc
     9901 acatcgcaga tctcctacac cgcgtccaag ggcggggtgc tcgccatgtc gcgcgagctg
     9961 ggcgtgcagt tcgcgcgcga gaacattcgg gtcaacgcgc tgtgcccggg tccggtggac
    10021 accccgctgc tgcaggagct gttcgcggct gatcccgagc gtgcccaacg caggctggtg
    10081 cacatcccga tgggccggtt cgcccggccc gaggagatcg caggcgccgt cgccttcctc
    10141 gccagcgacg acgcctcgtt catcacggcg tcgacgttcc tcgtggatgg cggcatctcg
    10201 ggtgcgtacg tgacgccgct gtagcggacg gacggatcag agcacctcga ggggtacctt
    10261 ccgatctccg ccatgtccgg ggcatcgaag ttgctcacgc cccactagcg gatccggcgg
    10321 ccatcaggtc ggagaagccg gccagggtct cctccagcgg gatcgccccc cgccagtgca
    10381 gcaggtacag gtccagccgg tcggacccag gcggcgcagg ctgtcctcgc aggcctgcac
    10441 cgtccccccg gcgggtggcg tggtgcggca gcaccttgct gaccaggaac acctcgtccc
    10501 gcctgccggc gatcgcctcg cccaccagca ccctcggcgg cgccgtcggc gtacatctcc
    10561 gcggtgtcga ccacgttgag ccccaggtcg agccccagcc gaagggacag gatctccgac
    10621 gcccggctgg cctgccgatc cgcgaagtgc cacgtgccct ggcccagcgc cgaaatcgtc
    10681 tcgcccgaag gcaggtccac ggaccggacc accgtcgtca tgccgacgac gatgacatgg
    10741 gaccggcacc gcagtgaccg gcccggcggg ccgttttcgc cgcccacggt cggctcgtgg
    10801 tgacgacgga cgcgctcagg cgtggtgcaa ccggtgcagc atcgccggca gcggctggct
    10861 gtgcacgacc gcgaggcgct gggtcgagcg ggtgagggcc acgtagaggt cgttggcgcc
    10921 gcgggatgac tcggcaagca gctcggcggg ctccaccacc acgacggcgt cgaactccag
    10981 gcccttcgcc gagttcaccg ggagcaccac cacaggtgcg ctgaggtcga tgtccggcgc
    11041 gtccgcgggc ggctcgccca ccaggcgctc ccgcaatgcc gccacccgcg cggtcggggc
    11101 gagcacggcg gtacgaccgt cgcccaccgc gccggcttcc tcggcgagca ccttcgcgac
    11161 cgcgtcgtcg agccggctcc cgtccacgcc cacggcccgg ggcggcaccc cggtgctgcg
    11221 gactgaccgg ggtgcggtga gtcccggttc gatcgcggcg agcacgtcgt cggccacgtc
    11281 cgcgatctcg gacggcgtgc ggtagttgac ggtcagctgt tcgagccgcc accggttcgc
    11341 cacgaacggc gagagcacct cgccccagga cgtcgcgccc gacaggtcac cggtctgcgc
    11401 gatgtccccc acgatcgtca tggaccggct ggggcagcga cgcatgatca gccgccaggc
    11461 catcggcgtg agctcctgcg cctcgtcgac gatcacgtgg ccgtaggtcc actcccggtc
    11521 ggccgcggcc cggtcggcgg tggagtcgta gcggcgcacc gcctgccgct cggcaagccg
    11581 gtcggcgtcc acgacgtcgg tggcgcgcag cagctcgggg tcgagatcct cctcgaggtc
    11641 cagcacgtcg aggaccccct gtgcgtacat cacctcctcg cgcagcgcgg ccgcctcccg
    11701 cgccgccgcc tcggcgccgt cgtcaccgag cagctcggcg gcctcgtcga gcaacgggac
    11761 gtcggcgggc gtccagcgct ggttcggcgg gacgtcggca gcgggtgcgg ggcgctgcag
    11821 cagctcccgg tcggcggccg ggagccgccg ggccaccgtg ttgagccgcc tcggggaggc
    11881 gaacagatcg gtgagcagct gctcggccga cagcgtcggc cacagggtgt cgagctcacg
    11941 ggtgagcgcc ctgctctcgg tgagctcctc gcggatgtcg gcgagatcgc cggcgtcgag
    12001 caggccgcgt ccccccagcg tgcgcgccac ctgctcggcc agcaggcgga ccgcctcctt
    12061 gtggaaggtg cgcttggcct cgttgtgcgg cttgcgcgaa cgccgggcgc gtgtgcgggc
    12121 ggcgttggcg atgtcgcgat cgagccggac ccgctgctgc tcgacgacga gttcgatggg
    12181 acgccggggc agctgctggc ggtcgcggac ggctgcggcg atcaccggcg ccatctctgc
    12241 ccggcccttc accgcggcgg tggccgcggg ttcggcgcgg cgcgcgtcga gacccgggtg
    12301 gagctgcccg acggtcccga gcaccacgct tgtctccccc agcgagggca ggacctggcc
    12361 gatgtagctc aggaacgtcg ggttgggccc gacaaccagc acaccgcggc gggccagccg
    12421 gtcgcggtgg gtgtagagca ggtatgcggc gcggtgcagc gcgacggccg tcttgcccgt
    12481 ccccgggcca ccctgcacga cgagcacccc ggacggcttg gagcggatga tcgtgtcctg
    12541 ctcggcctgg atcgtggcca cgatgtcgga catccgcccg gtccgccggg agtcgacggc
    12601 ggcgagcaac gccgcctcgc tcgtcagccc cccggcctgc gagacggccg tggcgtcgtc
    12661 cagatcgagc acctcgtcgt ccaccgcggt cacctcgcgg cgccgggtgc gcaggtgccg
    12721 ccgccgccgg atgccctcag gggacgcagc cgtggccgtg tagaacgggc gtgccgctgg
    12781 tgcgcgccag tccatcagca gcggttcgta ctcgttctcc tcgtccagca gcccgagccg
    12841 cccgacgtag cgccgctcgc cgtcgtcggt gtcgagccga ccgaacgcca gcccgtgctc
    12901 cgcggcgcgc agggccgcga gccgatcggt gtacatggcg gctgcggcgt cgcgctccgt
    12961 gagcgcctgc ggcgtgccca ccgtctcgtc gtgcagcacg ctgtggagac gctgcgcggt
    13021 atcggcgcgc cgggcgtcga ggcgctcata gagcatgcct acgtactggc gctcgtactc
    13081 gagaccgtcc tcggcagagg tgtgcctcgt ggcgtcggtg gtgatgtcgt cggtggtgat
    13141 gtcgtcggtg gtgatgtcgc tgccgctacg agcgggggac gtgctgttcg acaccggcaa
    13201 ttcaccttcg cgaagcgggc ggacggaatg gcgacttacg aacgtaccag cgcccgcgtt
    13261 ttcccccgcc aggccgcggg tagctcacac ggtgcgttcc gaccactgcc acacctggct
    13321 ggcgtgtcgt gtctgaatcg tcccgcgact ccgactacgc ccacctggcg ccgctgttcg
    13381 ccgagttcgc gtcccttccg ccggacgacc ccggccgggt cgcactgcgt gggaagctcg
    13441 tgacagggta cctgcccgtg gcgcaacata tcgcccgtcg cttcgccggc cgcggcgaac
    13501 cggtggacga tctcgagcag gcgggcaccg tcggcctgct caacgcggtg gaccggttcg
    13561 agcccgaccg cggcatcgac ttcctgtcgt acgcggtacc gaccatcacg ggcgagatcc
    13621 gacggcactt ccgggaccgc acgtggtcga tgcgggtgcc tcggcggctc aaggacctgc
    13681 agggtgcgat caacggcgcc atcggcccgc tctcacagga gctcgggcgg gcgccgcggc
    13741 ccagcgagat cgcggcgcgc ctcggcatcc cgaccgagga ggtcgtcgag ggtctggacg
    13801 cgcagcaggc ctatcgcagc acctcgctcg acgagctcgt ggccggtgct gacaccacct
    13861 tgaccgacac cctcgggagc gccgacaccg agctcgagaa ggtcgagtac cgggagacgc
    13921 tcgccccact gctcgacgag ctgcccgagc gcgagcggac gatcctgctg ctgaggttct
    13981 tcggcaacct cacccagacc cagatcgccg accacgtcgg catctcgcag atgcacgtgt
    14041 ctcggctgct gtcccagacg gtcgcacagc tacgacgccg gatgaccgac gacgggtagg
    14101 cgggcgcgga ccgggtaaac gaccgccatg accacgaatg tgctgcacag tatcgatgtc
    14161 gccgtgccgg tcagcacggc ctacaaccag tggacgcagt tcgagtcgtt cccgcggttc
    14221 atggacggcg tggagcgcat cgaacaggtg accccgacca ggacgcactg ggtcaccaag
    14281 atcgccgggg tcgagcgaga gttcgacgcc gagatcaccg agcagaaccc ggacgagcgc
    14341 gtggcgtgga cgaccgagaa gggcacgcac caggcgggtg tcgtgacctt ccaccgcatc
    14401 gacgccgccc acacccgcgt caccctgcag ctcgatcacg acccgcaggg tctggtcgag
    14461 aaggcgggcg atgcgctggg catcgtccag cgccgggtga agggcgatct ccagaacttc
    14521 aaggagttca tcgagacgcg tggcagcgag gacggcggct ggcgtggtga cgtgccgcgc
    14581 agcccgcaag ccggggacac gacgccgccc gcctgacccc gaggcagtcg aagcgaccta
    14641 gagacgggcc gctccccggt ctccgggggg cggcctctcc gcattcgccg gtgaccggcg
    14701 caggagccgg agcggaaggg cgaaccacat cgcgagcagg acgaccccga cggcggcggc
    14761 gaccgatatc gcgaacgctc ggttgaccgc gacgtcgagc acgagcagaa ccgcaccggt
    14821 gagggtgagc gccaacgtgg ccagcccgac ggtgaacagc cggtgaccca tctccacggt
    14881 ctcctgcttc accccgcggc ggaacgtcac gcggtggact gcagccggcg cgaccagcaa
    14941 cccggcggtg acgaccgaca gcagcaacgt gacgacgtag gtccagcgct gcaccgcgtc
    15001 gatctcggcg aagcgatcgg tgaacgacag cgacagcagg aacgcgaaga ggatctgcac
    15061 cccggtctgc gccacccgca gctcctgcag caactcgagc atgtttcggt cggcccgctg
    15121 cagcgggccc tcgcccggcc gctcggaccc ggctgacgac gcaccggtgt ccgggccctc
    15181 catgggcgga gggtacccgg acaccggatg gaggcgcggt gctcagctca cggttgcccc
    15241 ctcctcgtgg acctggtgcg ccccctccgt gaactcctcg acgagcttcg cgcagaaggc
    15301 cggcaggtcg tcggggttgc ggctcgacac caggccgtgg tcggtgacga cctcctcatc
    15361 gaggacggtg cccccggcgt tgcggatgtc ggtgcgcagg ctcgggtacg aggtgagggt
    15421 ccgtccccgc acgaggtcgg cttcgaccag cgtccacggc ccgtggcaga tcaccccgac
    15481 cggtttgccc gagcggaaga tctcctgcac gaaagcgacg acgtcggggc tcatccgcag
    15541 acggtcggcg ttcacggtgc cgccgggcat gatcagagcg tcgtagtcgg cggccgacgc
    15601 ctccttcgcc gacctgtccg cggtgaaggt gtcggcgggt tcgatgtcgt tgttcatcgc
    15661 ctggatctcg ccctcggaga gcgagacgat ctccgtccgt gctccctcct gggtcaccgc
    15721 atcgcgtggg cgcgtgagct ccacctgctc gacgccgtcc gtggccagga tcgcgatgcg
    15781 cttgccggcg agcttgcctg ccatgagctt cctttcctcg atgatggtgt gctgatcgcg
    15841 tacccgcgcc ggagcgcggg tatgcggtgc tcatga
//
LOCUS       DASRSC010000171         7950 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_877912,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000171 DASRSC010000000
VERSION     DASRSC010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7950
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_877912"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1804
                     /locus_tag="VFE39_08530"
     CDS             <1..1804
                     /locus_tag="VFE39_08530"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HET6258683.1"
                     /translation="ARVFLDHVRPELEAEGICILRWGDLSDDQRARLSAYFSAQVFPV
                     LTPLAVDPAHPFPYISGLSLNLAVTVRDPEGRTERFARVKVPNNVPRLVRVRTDDDSI
                     TFLPIEDLISAHLGSLFTGMELAEVHAFRVTRNADVEVEEDRDEDLLQSLERELARRR
                     FGPPVRLEVTDTMSEHVLELLLRELDVHPGDVVTVPGLLDLTALWAVHAVDRPDLKDE
                     PFVPATHPAFADRETPRSVFATLREGDVLVHHPYDSFSTSVQRFIEQAASDPNVLAIK
                     QTLYRTSGDSPIVDALIDAAAAGKQVVALVEIKARFDEQANIRWARELEKAGVHVVYG
                     LVGLKTHCKTSLVVRQEGSTIRRYCHIGTGNYNPKTARLYEDLGVLTADPTIGADLTD
                     LFNSLTGYSRQTSYRSLLVAPYGVRRGIVRRIEDEIDAHRAGDSNARIRFKLNSLVDE
                     QVIDALYRASQAGVPVEVVVRGICALRPGRPGLSDNIHVRSILGRFLEHSRVFHFGAA
                     DEYWIGSADMMHRNLDRRVEVLLRVADPSLADKLGEMFDSCLDPATRCWTLHADGSWE
                     PSPPPGSGVSPVRDHQAEMMMRHAAVAMALAESE"
     gene            1801..2739
                     /locus_tag="VFE39_08535"
     CDS             1801..2739
                     /locus_tag="VFE39_08535"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6258684.1"
                     /translation="MSGAAHADRRDSTVGLDADVLAAGAVLWRHGTQSPELALVHRPR
                     YDDWSFPKGKVDPGETMPFTAVREVAEETGQAARLGPVLDDVRYTVPEGRKLVRYWAA
                     EALGGEFVPGDETDELRWVDPAHAAEMLSYRHDVDVLERFTALGPPASMVLLVRHAKA
                     GSRHQWDGHDMLRPLSETGREQARQLARLLALFGPDRIASASPVRCRQTVAPLAEKLG
                     LPVLDEPLLGETEFAQDPEAGLARFRELAAEPGVSVLCSQGGVIPHIVGALATAAGLP
                     GVDPDDVPSRKGSTWALAFGEDTTLRAADYYPRPTG"
     gene            complement(2746..3261)
                     /locus_tag="VFE39_08540"
     CDS             complement(2746..3261)
                     /locus_tag="VFE39_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET6258685.1"
                     /translation="MNKTQLIDALAARIGDRRTAASAVDGLLETIIETVRAGDSVSLT
                     GFGVFETRARAARVARNPRTGEIVDVPATTVPAFRPGVGFRAAVGGAEVHATPAPRAT
                     PVRLVSVAAASAVAVAEPPAAEPSLKAKSDTSFATAAPGKKAGKSEKRDKGKQAKSKN
                     DKNDKNKGKKK"
     gene            complement(3309..3896)
                     /gene="leuD"
                     /locus_tag="VFE39_08545"
     CDS             complement(3309..3896)
                     /gene="leuD"
                     /locus_tag="VFE39_08545"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET6258686.1"
                     /translation="MEAFSTHTGIGVPLRRSNVDTDQIIPAVYLKRVTRTGFDDGLFH
                     AWRQQPDFILNVEPFSRGSVLVAGQDFGTGSSREHAVWALMDFGFRVVISSRFGDIFR
                     GNSAKAGLLAAEVSQPDVELLWKLLENDPGTEVTVDLTEKTVQARELVVRFEIDDYTR
                     WRLLEGLDDIGLTLRHAEDIAAFETARPARLPITT"
     gene            complement(3896..5308)
                     /gene="leuC"
                     /locus_tag="VFE39_08550"
     CDS             complement(3896..5308)
                     /gene="leuC"
                     /locus_tag="VFE39_08550"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HET6258687.1"
                     /translation="MGRTLAEKVWDQHLVRRGEGSEPDLLYIDLHLVHEVTSPQAFDG
                     LRLANRPLRRPDLTLATEDHNVPTIDVGLPIADAVSRTQVETLRKNCAEFGVPLYPMN
                     HAEQGIVHVVGPQLGLTQPGMTVVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQSLP
                     LRRFRTMAINVNSADGRLREGVTSKDVVLAMIAQISTGGGQGYVLEYRGNVIENLSME
                     ARMTICNMSIEAGARAGMIAPDETTFAYLKGRDRAPSGPQWDEAVAAWRELRTDDDAT
                     FDAEVHIDADALTPFVTWGTNPGQGLPLSASVPDPAGIVDENERASAEKALTYMGLKA
                     GTPLREVAIDTVFLGSCTNGRIEDLRAAAAVIKGKKVADGVRMLVVPGSMRVRFQAED
                     EGLHEIFTAAGAEWRSAGCSMCLGMNPDQLAPGERSASTSNRNFEGRQGKGGRTHLVS
                     PLVAAATAVRGTLSSPEDLN"
     gene            5362..6063
                     /locus_tag="VFE39_08555"
     CDS             5362..6063
                     /locus_tag="VFE39_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET6258688.1"
                     /translation="MRQHSGIGVLDKAVGVLRAVATEPCGLAELCQRTGLPRATAHRL
                     AVGLEVHGLLYRGADGRWRPGPALAELSAGGVDPLLEAAAAVLPRLRDITGESVQLYR
                     RDGVQRVCVATAEPASGLRDTVPVGTRLPMTAGSGAKVLAAWAEPSVQRAVLAEATFG
                     ERVLVDVRRRGWAQSVAERETGVASVSAPVRDGSGHVVAAVSVSGPVDRIGRRPGVRW
                     AADLLAAAEALQHRL"
     gene            6196..>7950
                     /locus_tag="VFE39_08560"
     CDS             6196..>7950
                     /locus_tag="VFE39_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET6258689.1"
                     /translation="MSYLLGIDVGTTRTAAAIGRSGTRPGDTEIVNLGDRSSAVPSVL
                     YIGDDGSVVVGEAAERRAVSNPDHVVREFKRRIGDPTPILVAGRPWAPEELSARLVRW
                     VVDRAAQREGGAAARIAVTIPASWGAHKKDRLGGALAGQGVPVTFIAEPEAAALHYAA
                     GERVEPGSTIAVYDLGGGTFDAAVVHKDGHGFTLLGRPEGLERLGGVDFDEVVFEHVL
                     QGLPELFDGLDDSDPAVLSAVAAVRRECTEAKEALSSDTEVSIQVMTPGGQGSVRLHR
                     SEFEAMIRPRVEETVEALRRAVGSAGLAPAQLSAVLLVGGSSRIPLVGQLVSEQLGRP
                     VAVDADPKNAIAMGAVLSMTPSSGTGAMPQVSSPRTGPHGRQPIGAAAAPLMGAAAGA
                     PPIGTGATPPIGVGGGPSPYAPGGNATHRTGVGAAAGANGGAAANQWMPGSGGPAAPM
                     PQRPAPAFPERPQLDMDDGRDFEYAERRPSLTPVRLVSIGGAVAAAAIVAAVFLWPTG
                     QAVPVDASVPSTIAPTSAGAEPTPAAPPEEQAPPNGETTHRSTPSATPKPAEQPPVVE
                     PSVAPSRQPEPTTTPPPTP"
BASE COUNT         1091 a         2867 c         2853 g         1139 t
ORIGIN      
        1 cgcacgcgtg ttcctcgacc acgtgcgccc ggagctcgag gccgagggca tctgcatcct
       61 gcgctggggc gacctgtccg acgaccagcg ggcgcggctc tcggcctact tctccgcgca
      121 ggtgttcccg gtgctcacgc cactggcggt ggacccggcc cacccgttcc cctacatctc
      181 cgggctctcg ctgaacctcg cagtcacggt gcgcgacccg gagggccgca ccgagcggtt
      241 cgcgcgggtc aaggtgccca acaacgtgcc gcggctggtg cgggtgcgca ccgacgacga
      301 cagcatcacc ttcctgccca tcgaagacct catctccgcc catctcggct cgctgttcac
      361 cggcatggag ctcgccgagg tgcacgcctt ccgggtcacg cgcaacgccg acgtggaggt
      421 cgaggaggac cgcgacgagg acctgctgca gtcgctggag cgcgagctcg cgcgacgccg
      481 gttcgggcct ccggtgcgcc tcgaggtcac cgacacgatg agcgagcacg tgctggagct
      541 gctgctgcgc gagctggacg tccaccccgg cgacgtcgtc accgtgccgg ggctgctcga
      601 cctcaccgcg ctgtgggccg tgcacgccgt cgaccggccc gacctcaagg acgagccgtt
      661 cgtcccggcc acgcaccccg cgttcgccga ccgggagacc ccgcgcagcg tgttcgccac
      721 cctccgggag ggggacgtgc tcgtccacca cccctacgac tcgttctcca cgagcgtgca
      781 gcggttcatc gagcaggcgg catccgaccc caacgtcctc gcgatcaaac agacgctcta
      841 ccgcacgtcc ggcgactccc cgatcgtcga cgccctgatc gacgccgccg cggcaggcaa
      901 gcaggtcgtg gcgctggtgg agatcaaggc gcggttcgac gagcaggcca acatccgctg
      961 ggcgcgcgag ctggagaagg cgggcgtcca cgtcgtctac gggctcgtcg ggctgaagac
     1021 gcactgcaag acctcgctcg tggtgcgtca ggagggctcg acgatccggc gctactgcca
     1081 catcggcacc ggcaactaca acccgaagac cgcacggctc tacgaggacc tcggcgtgct
     1141 caccgccgac ccgacgatcg gtgccgacct cacagacctg ttcaactccc tcaccggtta
     1201 ctcccgccag acgtcctacc ggagcctgct ggtggcgccc tacggcgtcc gccgtggcat
     1261 cgtccggcgg atcgaggacg agatcgacgc ccaccgcgcg ggcgactcca acgcgcggat
     1321 ccggttcaag ctcaactcgc tcgtcgacga gcaggtgatc gacgcgctct accgggcgtc
     1381 gcaggcgggt gtgcccgtcg aggtggtggt gcgtggaatc tgcgcgctcc gtccgggccg
     1441 cccgggcctg tcggacaaca tccacgtccg gtcgatcctc ggccggttcc tggagcactc
     1501 gcgggtcttc cacttcgggg cggccgacga gtactggatc ggcagcgcgg acatgatgca
     1561 ccgcaacctg gaccggcgcg tcgaggtgct gttgcgggtg gccgatccga gcctggcgga
     1621 caagctcggc gagatgttcg actcctgcct cgaccccgcc acccggtgct ggacgctgca
     1681 cgccgacggg tcatgggagc cgtcgcctcc cccgggctcg ggggtgtccc ccgtgcggga
     1741 ccaccaggcc gagatgatga tgcggcacgc tgccgtcgcc atggccctgg ctgaaagcga
     1801 atgagcggag cggcacacgc cgaccgtcgg gacagcacgg tcggcctgga cgccgacgtg
     1861 ctggcggcag gagccgtgct ctggcggcac ggcacccaaa gcccggagct ggcgctcgtg
     1921 cacaggcccc gctacgacga ctggtcgttc cccaagggca aggtcgatcc cggcgagacg
     1981 atgccgttca ccgccgtgcg ggaggtcgcc gaggagacgg gtcaggccgc ccggctcggc
     2041 cccgtgctcg acgatgtgcg ctacacggtg cccgagggca ggaagctggt gcgctactgg
     2101 gcggcagagg cgctcggcgg cgagttcgta ccgggggacg agaccgacga gctgcgctgg
     2161 gtcgatccgg cgcacgctgc cgagatgctc tcgtaccggc acgacgtcga tgtgctggag
     2221 cggttcaccg ccctgggacc gcccgcgtcg atggtgctgc tcgtgcggca tgccaaagcc
     2281 ggcagccggc accagtggga cggccacgac atgctgcgac cgctgtcgga gacgggccgc
     2341 gagcaggccc ggcagctggc ccgcctgctg gcgctgttcg ggccggaccg gatcgcgagt
     2401 gcgtccccgg tgcgttgccg ccagaccgtg gcgcctctgg ccgagaagct gggcctcccg
     2461 gtactcgacg agccgctgct cggtgagacc gagttcgcgc aggaccccga ggccgggctc
     2521 gcccggttcc gggagctcgc cgcggagccc ggcgtcagcg tgctgtgcag ccagggcggc
     2581 gtgataccgc acatcgtcgg cgccctcgcc accgccgcag gcctgccggg ggtggatccc
     2641 gacgacgtcc cgtcccgaaa gggcagcacc tgggcgctgg ccttcggcga ggacacgacg
     2701 ctccgggcgg ccgactacta cccccgcccc accggctgaa gcccgctact tcttcttgcc
     2761 cttgttcttg tcgttcttgt cgttcttcga cttcgcctgc ttgcccttgt cgcgcttctc
     2821 cgacttcccc gccttcttgc cgggagcggc cgtcgcgaac gatgtgtcgg acttcgcctt
     2881 gagcgacggc tcggcggccg gcggctcggc cacagcaacg gcggacgccg cagccacgct
     2941 cacgaggcgg accggcgtgg cgcggggagc cggggtggcg tggacttccg cgccgccgac
     3001 cgcagcgcgg aagccgacgc cgggacggaa cgccggcacc gtggtcgcag gcacgtcgac
     3061 gatctcgccg gtgcgcgggt tgcgcgccac tcgggcggcg cgcgcgcgtg tctcgaagac
     3121 cccgaacccg gtgagcgaca cggagtcgcc ggcgcgcacg gtctcgatga tggtctcgag
     3181 gagcccgtcg acagcgctgg ccgctgtgcg acgatcaccg atacgcgccg ccaacgcgtc
     3241 gatgagctgc gtcttgttca tgacaagacg gtacccgccg cgccggtagc ccggcgggca
     3301 cgccctgatc aggtcgtgat cggcaaccgc gccgggcgcg ccgtctcgaa agccgcgatg
     3361 tcctcggcgt ggcgcagcgt caacccgatg tcgtcgagcc cctccagcag ccgccagcgg
     3421 gtgtagtcgt cgatctcgaa ccgcacgacg agctcacgcg cctgcaccgt tttctccgtc
     3481 aggtcgacgg tgacctcggt gcccgggtcg ttctccagca gcttccagag cagctccaca
     3541 tcgggctgcg acacctccgc ggccaggaga cccgccttcg ccgagttgcc gcggaagatg
     3601 tcgccgaacc gggaggagat gaccacccgg aacccgaagt ccatgagcgc ccagaccgcg
     3661 tgctcccgcg aggaaccggt gccgaagtcc tgccccgcca ccagcaccga gccgcgcgag
     3721 aacggctcga cgttgaggat gaagtcgggc tgctgacgcc aggcatggaa gagcccgtcg
     3781 tcgaacccgg tgcgggtgac ccgcttgaga tagaccgcgg ggatgatctg gtcggtgtcg
     3841 acgttggacc ggcgcagcgg cacgccgatg cccgtgtggg tgctgaatgc ttccatcagt
     3901 tcaggtcctc cggagagctc aacgtcccgc gcacggccgt ggccgcggcc acgagcgggg
     3961 acacgaggtg ggtccggccg cccttgccct gccggccctc gaagttgcgg ttcgacgtcg
     4021 atgcgctgcg ctcgcccggc gcgagctggt cggggttcat ccccaggcac atggagcaac
     4081 cggcggatcg ccactcggcg cctgccgcgg tgaagatctc gtgcagcccc tcgtcctcgg
     4141 cctggaaccg cacccgcatc gaacccggca cgaccagcat gcggacgccg tcggccacct
     4201 ttttgccctt gatcacggcg gcggctgccc gcaggtcctc gatgcgccca ttggtgcagg
     4261 agccgaggaa caccgtgtcg atggccacct cgcgcagcgg ggtgcccgcc ttcagaccca
     4321 tgtaggtgag cgccttctcc gcggaggcgc gctcgttctc gtccacgatg ccggccgggt
     4381 cgggcacgct cgcggacagc ggcagtccct ggccggggtt ggtgccccag gtgacgaacg
     4441 gggtgagggc gtcggcgtcg atgtggacct cggcgtcgaa cgtcgcgtcg tcgtcggtgc
     4501 gcagctcacg ccatgcggcc acggcctcgt cccactgcgg cccggacggc gcgcggtcgc
     4561 ggcccttcag gtaggcgaac gtggtctcgt cgggcgcgat catcccggcc cgtgcgcccg
     4621 cctcgatcga catgttgcag atcgtcatcc gggcttccat cgagaggttc tcgatgacgt
     4681 tgccgcggta ctcgagcacg tagccctgtc caccgcccgt gctgatctgg gcgatcatcg
     4741 cgagtacgac gtccttgctg gtgacgccct cgcggaggcg cccgtcggcc gagttgacgt
     4801 tgatcgccat cgtccggaac cgccgcaggg gcagggactg ggtggcgagc acgtgctcca
     4861 cctcggaggt gccgatgccg aaggcgaggg ccccgaacgc gccgtgcgtg gaggtgtggc
     4921 tgtcgccgca gaccaccgtc atgcccggct gggtgagccc gagctgcggg ccgacgacgt
     4981 gcacgatgcc ctgctcggcg tggttcatcg ggtagagcgg cacgccgaac tcggcgcagt
     5041 tcttccggag cgtctccacc tgcgtgcgcg acaccgcgtc ggcgatcggc agcccgacat
     5101 cgatggtggg gacgttgtgg tcctcggtgg caagtgtgag gtcgggcctg cgcagggggc
     5161 ggttggcgag ccggagcccg tcgaacgcct gtgggctcgt gacctcgtgc accaggtgca
     5221 ggtcgatgta gagcaggtcg ggctcgctgc cttccccgcg acggaccagg tgctggtccc
     5281 agaccttttc cgccagcgtg cgccccatga ccatcacctc ctcactggat gtctcagatg
     5341 tcgagaagtt agtatcgtct tgtgagacag catagcggca tcggcgtcct cgacaaggcc
     5401 gtgggcgtgc tgcgcgccgt tgccaccgag ccgtgcgggc tggccgagct gtgccagcgc
     5461 accggtctgc cccgggccac cgcgcaccgg ctggccgtgg ggctcgaggt gcacgggctg
     5521 ctgtaccgcg gggccgacgg gcggtggcgt cccggtcccg cgctcgccga gctctccgcc
     5581 ggcggcgtcg acccgctgct cgaggcggcc gccgccgtgc tgccccggct gcgcgacatc
     5641 accggggaga gcgtgcagct ctaccgccgg gacggggtgc agcgcgtctg cgtggccacg
     5701 gcggagccgg ccagcgggct gcgcgacacc gtgccggtcg gcacgcggct gccgatgacc
     5761 gccgggtccg gggccaaggt gctggcggcg tgggcagagc cctccgtgca gcgcgcggtg
     5821 ctggccgagg ccacgttcgg cgaacgcgtg ctcgtggacg tccgcaggcg cggctgggcc
     5881 cagagcgtgg ccgagcgcga aaccggggtg gcgagcgtgt ccgcccccgt gcgggacggc
     5941 tccggccacg tcgtcgcggc ggtttcggtg tcggggcccg tggatcggat cggcagacga
     6001 ccgggcgtcc ggtgggccgc cgacctgctt gcggcggcgg aggcgctaca gcaccgactg
     6061 tgagcgccac agtattacga cggatgtcac agagggaccg atacccgcat gatcgatgcg
     6121 aacacgggcg gctaacgtga tcggcactct gctcgatgga caagcagagt cgttcggaca
     6181 cgaagacggg tgacgatgag ttatctgctc ggcatcgatg tgggcaccac ccgcaccgcc
     6241 gccgcaatcg gccggtcggg cacccgcccg ggtgataccg agatcgtcaa cctcggcgac
     6301 cgctccagcg ccgtcccctc cgttctctac atcggggacg acggctcggt ggtcgtgggc
     6361 gaggcagccg aacggcgggc ggtgagcaac ccggaccatg tggtgcgcga gttcaagcgc
     6421 cggatcggtg accccacccc gatcctggtc gcgggccggc catgggcccc ggaggagctg
     6481 tcggcgcggc tggtgcgctg ggtggtcgac cgggccgcac agcgcgaggg cggcgcggcc
     6541 gcccgcatcg ccgtcaccat tcccgcgtcg tggggcgccc acaagaagga ccggctgggc
     6601 ggagcgctcg ccgggcaggg cgtgcccgtc acgttcatcg ccgagcccga ggccgcggcg
     6661 ctgcactacg cagcgggcga gcgggtggag cccggctcga cgatcgcggt gtacgacctc
     6721 ggcggcggca ccttcgacgc cgccgtcgtc cacaaggacg gccacggctt caccttgctg
     6781 gggcgccccg agggcctcga gcggctcggc ggcgtcgact tcgacgaggt cgtgttcgag
     6841 cacgtcctcc aggggcttcc cgagttgttc gacgggctcg acgactccga tccggcggtg
     6901 ctgtcggcag tggcggcggt tcggcgcgag tgcaccgagg ccaaggaggc actcagcagc
     6961 gacaccgagg tctcgatcca ggtcatgacg ccgggcggcc aagggtcggt gcggctgcac
     7021 cgcagcgagt tcgaagccat gatccggccc cgcgtcgagg agaccgtcga ggcgctgcgg
     7081 cgcgctgtcg gctcggccgg gctcgcaccg gcacagctgt cggccgtcct gctggtcggc
     7141 gggtcctccc gcatcccgct cgtgggccag ctcgtctccg agcagctggg gcgtccggtc
     7201 gcggtggatg ccgacccgaa gaacgccatc gccatgggcg ccgtgctgtc gatgacaccg
     7261 tcatccggca ccggcgccat gccgcaggtg tcgtccccgc gcaccggccc gcacggccgc
     7321 cagccgatcg gcgccgcggc ggctcccctc atgggggctg ctgcgggggc accaccgatc
     7381 ggcacagggg ccaccccgcc catcggtgtc gggggcggtc cctcgccgta cgcgcccgga
     7441 ggcaacgcca cgcaccggac cggcgtcggc gccgccgccg gtgcgaacgg gggcgctgcc
     7501 gccaaccagt ggatgccggg ctccggtggg ccggcggccc cgatgccgca gcgcccggcg
     7561 cccgccttcc cggagcggcc gcagctggac atggacgacg ggcgcgactt cgagtacgcc
     7621 gagcgacgac cgagcctgac ccctgtgcgc ctggtgagca tcggcggcgc cgtcgccgcg
     7681 gcggccatcg tcgcggcggt gttcctctgg ccgaccgggc aagcggtccc ggtcgatgcg
     7741 agcgtgccga gcaccattgc gcccacctcg gcgggggcgg aacccacccc cgccgccccg
     7801 cccgaggagc aggccccgcc caacggtgag acgacccacc ggtccacgcc gtcggcgacg
     7861 ccgaagcctg ccgagcagcc tccggtcgtc gaaccgtcgg tggcgccgtc tcggcagccg
     7921 gagcccacca cgacaccacc tccgaccccc
//
LOCUS       DASRSC010000172        11655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5464917,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000172 DASRSC010000000
VERSION     DASRSC010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5464917"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..157)
                     /locus_tag="VFE39_08565"
     CDS             complement(<1..157)
                     /locus_tag="VFE39_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HET6258690.1"
                     /translation="MRAMWSGAVSFGLVSVPVKAYSATTNHYIRFHQVHAADGGRIKY
                     RRTCSIDG"
     gene            244..1155
                     /gene="ligD"
                     /locus_tag="VFE39_08570"
     CDS             244..1155
                     /gene="ligD"
                     /locus_tag="VFE39_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET6258691.1"
                     /translation="MLATPGALPVGPEWLFEVKWDGMRLLVDVADGQVRLTSRTGRDV
                     TANFPELADLTRLAPDVLLDGEVVLLEGGVPSFAALQDRMHGPVLHRTAQARPVTYMV
                     FDVLRLYGVSLLDRPLEERRGTLERLDLAPLPILSLSPAYTDGRALLEATRQRGMEGV
                     VAKRRDGVYRPGRRSPSWTKVTHRQTQVCVVGGWRPERSSANRIGALLLGVPDGAGLR
                     YAGRVGSGLAGETAQRVLRERLIEVDRTPFTVRLPRSDGVGAHWCEPLTVVEVAHTGW
                     TDGGRLRQPVFRGVRDDLEPEQVRRED"
     gene            1329..1937
                     /locus_tag="VFE39_08575"
     CDS             1329..1937
                     /locus_tag="VFE39_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HET6258692.1"
                     /translation="MTTMLTWMADDGHGLEGARLLLHTSGFRALGRMVRAEPGGDFTA
                     SYRLIVGEDAGLERLSVTAATAERERHLTVNRTDDGVWLLDTGTGGGSTRTDFVGAVD
                     VDLEFSPMFNTIPIRRLGLHREAGEHTVPMVFVSLPDLGVRVVEQTYRTVSELDDATG
                     QAVVEFRSGDFTAELVVDADGAVVNYPGLARRLLAGVTPAAG"
     gene            complement(1941..2885)
                     /locus_tag="VFE39_08580"
     CDS             complement(1941..2885)
                     /locus_tag="VFE39_08580"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET6258693.1"
                     /translation="MTERAVCVIGTGLIGGSLLRAADKAGREVWGTSGSAATAEQARA
                     DGFDVTTDTDAALRRAAESDALVVLAVPLTVVPEVLRRVDAAAPTCRLTDAVSVKVAV
                     ADAVARYAPRSRYVGGHPMAGTAESGWVAGRAGLFADAAWVVVADDGLDLEVWADVAE
                     LAWACGARVVPAAADEHDLAVARVSHLPHLLAAVLAAVGAAGEDDLPLALAASSFADG
                     TRVAGTRPELVLAMCEGNRDALLAAVDDALGRLGAMRGALASTGGLAATVHAGHAGRQ
                     RWAASRNPDGMRVPRGRTSLSALRDIGRRGGAVVGWAG"
     gene            complement(2959..4287)
                     /locus_tag="VFE39_08585"
     CDS             complement(2959..4287)
                     /locus_tag="VFE39_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET6258694.1"
                     /translation="MITSLRDLGLTLRSGELSPVEHVTEVLDRLATDSHNAVITLDAE
                     RAVAQAAALTAELARGEWRGPLHGVAIGVKDLFDVAGLPTRAGSRVLADAPAATADAP
                     VVARLRAAGAVVVAKLHTHEFAYGPTGDVAATGPARNPHDPSRITGGSSSGSAAAIAA
                     GHLPLALGTDTGASVRTPAALCGVVGLKPAFGALSTEGVFPLSETCDHVGVLAADVHA
                     ASVAWDVLSRPDGTGGGIQAPGVAGVRVGVLVDDYWRAFDPALNAAVDAAAETLRARG
                     ASVVEVSTPMVGELAATYPVIVGAEAYATHSTWLAERPQDYQDATRERLLLWADQPAR
                     EYVDALRAKRRLAAAVRAAVADVDVLLLPTTRLRATAIGVDAIDVDGVAVPVRPQLLD
                     LTLPFNLTGWPAVSVPGRVADGGLPAGVQVVGVRFEERGVLRVAGALARE"
     gene            4413..4997
                     /locus_tag="VFE39_08590"
     CDS             4413..4997
                     /locus_tag="VFE39_08590"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HET6258695.1"
                     /translation="MTTALVVIDVQRGFDDPYWGPRNNPDAEENIAALLDGWRATGRP
                     VVLVHHDSLTPGSPLAAGTPGNGFAAILDGVQPALTFGKNVNSAFHGEIDLDAWLREH
                     DIEAVVLVGIQTNMCVETTARVGGNLGHRVTVAIDATFTFDLAGPDGAVLTAEELTRA
                     TAVNLHGGGFARIATTTEVLAELGQASASRANPA"
     gene            complement(4964..6178)
                     /locus_tag="VFE39_08595"
     CDS             complement(4964..6178)
                     /locus_tag="VFE39_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET6258696.1"
                     /translation="MSLDAAQITGLLTQARGVQPRTVALRRAVHRHPELGLELPRTRN
                     AVVTALADLPLQVHLGRAVSSVVAVLEGHRSGPTILLRGDMDALPLAEDTGLAFASQV
                     SGAMHACGHDTHVAMLASAARVLATRRDELAGRVLFMFQPGEEGFHGARHMIDEGLLD
                     RTGPAGRPDAAYALHISATVPSGEVHVRPGPLMAAADTIRVTVSGRGGHASAPHDALD
                     PLPAAAAMVGALQTAVTRRVDTHHPVVLTITRIVAGTTTNIIPETAFMEGTVRTLSEA
                     SRATVHDEIRRVCRYTAMAHGCTAEVEIEPGYPVTVNDDEAADRLGRLADTVVGRDRV
                     VTMPTPIMGAEDFSYVLAEVPGALAFLGGCPPGVHPGAAAPNHSNRVVFDEAAMAAGV
                     AVYAGLALDALA"
     gene            6270..6791
                     /locus_tag="VFE39_08600"
     CDS             6270..6791
                     /locus_tag="VFE39_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine deaminase-associated protein"
                     /protein_id="HET6258697.1"
                     /translation="MAVQSVEARADVREQALPGYAVAAIREDGQWRCTRMTPEALVDL
                     DTAITELRELRSTGAVLGLLDVDDEFFVLLRPSPGGIALMVSDAVAALDYDVAADVLD
                     LLRLELPPEEEALDSPPWPEGDLAILADLGLPADELEVLAGEVELYPDEQLAAIGRRC
                     GFADALAEVVDAG"
     gene            6851..7288
                     /locus_tag="VFE39_08605"
     CDS             6851..7288
                     /locus_tag="VFE39_08605"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET6258698.1"
                     /translation="MLVRRAIEIAAAAPRTGDVPIGAVVVDAEGRELAAACNGREALG
                     DPTAHAELLALRAAAAVRGEWRLEGCTLAVTVEPCTMCAGAIGLARVARVVFGAWEPK
                     TGAAGSLWDVLRDRRLNHRPEVVAGVLAQECAALLESFFAGYR"
     gene            7373..7795
                     /locus_tag="VFE39_08610"
     CDS             7373..7795
                     /locus_tag="VFE39_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6258699.1"
                     /translation="MEQLVQRTRVGAYVVCVQDDALLLVRFSGSDRWTLPGGGLDHGE
                     RPEDAAVREVDEETGLQVVLGPLIGVDSTRWDRASDGRPIDMHALRILYTGEVVGGSL
                     RDEIGGSTDRAEWVRLAVVAARPRARLVGIGLAAAGHA"
     gene            complement(7799..8167)
                     /locus_tag="VFE39_08615"
     CDS             complement(7799..8167)
                     /locus_tag="VFE39_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HET6258700.1"
                     /translation="METSAAAKLLLEEEASARLAEHLDALPEAPVSCLVLETELRRLA
                     VRVGLPQVAVTSVLERFDLLETDRSLFREAGLLPGPHLRSLDALHVAAALRLDAGVML
                     SYDRRQIAAAEAVGLRTLAV"
     gene            complement(8176..8430)
                     /locus_tag="VFE39_08620"
     CDS             complement(8176..8430)
                     /locus_tag="VFE39_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HET6258701.1"
                     /translation="MARTIPHRELRNNSSAVLREVQAGETIAVTNNGEVVAVLIPPPR
                     RPGAVLPIRRALVRGGFADLPRVRLDHPLQDDLDDLRGDR"
     gene            complement(8459..9208)
                     /locus_tag="VFE39_08625"
     CDS             complement(8459..9208)
                     /locus_tag="VFE39_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET6258702.1"
                     /translation="MTPVLEVRGLVKRYGAATVLGGVDLTVDEHQVVALIGTSGSGKS
                     TLLRCVDLLEEIDDGQVLLDGVDVSDPRSDVAGAQRRMAIVFQAFNLFPHMTAAQNVA
                     LAPRVVHRVPAAEADERARDLLRRVGLESFAGAYPDRLSGGQQQRVAIARALAVQPRL
                     LLLDEITSALDPELVGEVLMIVRELAGGGMTILMATHEMGFAKQVSDRVCFLDGGRVL
                     EAGPPGQVLGAPVHERTRQFLTRVIAAGRLA"
     gene            complement(9205..10059)
                     /locus_tag="VFE39_08630"
     CDS             complement(9205..10059)
                     /locus_tag="VFE39_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET6258703.1"
                     /translation="MTEPPVATELSPLARERLAYRRSRARRSTLVAFASTVVFAGAVA
                     LGVASSPGWPRVRDTFFDVHVAWSSLPSILEGLWLNIRVLVVAEIGILVLGLVIAVLR
                     TLRGPVFLPVRALATGYVDLFRGVPLLIALYLIGFGVPALRLQGMPTDTAVLGTATLI
                     LVYSAYVAEVFRAGIESVHPSQRAAARSLGLTHRQSMRLVVLPQAVRRVLPPLLNDFV
                     ALQKDVGLISVLGAVDAIRAAQIEAARTFNFTPYVVAALLFVLLAIPTGRVADTLAAR
                     AARRQGWS"
     gene            complement(10056..10910)
                     /locus_tag="VFE39_08635"
     CDS             complement(10056..10910)
                     /locus_tag="VFE39_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6258704.1"
                     /translation="MTPPLPVRRLLAAAVVVLAAACAPADTNTPPAAASCVPGALPTR
                     TAGTLTVGTDQPVYPPWYLDDDPTSGRGFESAVAYAIAGELGYPRDTVTWTRVPFNAA
                     VQPGPKAFDLNLTEFSITDERRQAVDFSAPYYDVKQAVVALGSSPFAGATTIAGLKDA
                     RLGAQVGTTSYEAIVSQLAPSSQPAVFNTNDDAKLALTNGQVDAIVVDLPTAFFITSA
                     ELDGGKIIGQLPAGIGTPEQFGAVLDKGSPLTGCVSDAVERLRAAGTLAALEQQWLAT
                     AGSAPELR"
     gene            complement(11035..>11655)
                     /locus_tag="VFE39_08640"
     CDS             complement(11035..>11655)
                     /locus_tag="VFE39_08640"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6258705.1"
                     /translation="KEFGAKAFKFYNVRYDYGSPFPWRMDDPKIAFPIFEKAQELGIN
                     LIGVHKGVPLGPQPIEHTRTWDMDGAAANFPDINFVIFHVGLPFIDETCWQLIRYPNL
                     HASLAATINFLVRAPRMFAEIIGKLLFWCGEDKIVYGSEAPIFHPQWAIKAFMDFQIP
                     QDLCDGYGYPQLTDQAKRKILGENLLRLHGMDVAETTARLGRQQAH"
BASE COUNT         1641 a         4185 c         4246 g         1583 t
ORIGIN      
        1 caccgtcgat cgagcacgtg cgcctgtact tgatccgccc accatcggcc gcgtgcacct
       61 ggtggaaccg gatgtagtga ttggtcgtgg ccgagtacgc cttgaccggt acgctgacca
      121 gaccgaacga cacggcgccg ctccacatcg cgcgcatgca ggcccctgtt ccttccgcga
      181 gcaaacctcg ccagcaacac tgtgtgaatc tctaccgggt gccagcatga cggcgtgcag
      241 cccatgctcg ccacccctgg agcccttccg gttggtccgg aatggctgtt cgaggtgaaa
      301 tgggacggca tgcggctgct cgtcgacgtg gccgacggtc aggtccgcct caccagtcgc
      361 accgggcgtg acgtcacggc gaacttcccc gagctcgccg acctcacccg gctcgcgccc
      421 gacgtcctgc tcgacggtga ggtcgtcttg ctcgagggcg gcgtcccgag cttcgcggcg
      481 ctgcaggacc gcatgcacgg ccccgtcctg caccgcacgg cacaggctcg gcccgtgacc
      541 tacatggtgt tcgacgtgct gcgcctctac ggcgtgtcgt tgctggatcg cccgctcgag
      601 gagcgccggg gcaccctcga gcgcctcgat ctcgcccccc tgccgatcct ctccctctcc
      661 ccggcctaca ccgacggccg cgctcttctc gaggccacgc gtcagcgggg catggagggt
      721 gtggtggcca agcgccgcga cggcgtctat cggccggggc ggcggagccc gagctggacg
      781 aaggtcaccc accggcagac gcaggtctgc gtcgtcggcg gctggcggcc ggaacggagc
      841 agcgcgaacc ggatcggggc gctcctgctc ggcgtccccg acggcgccgg gctgcgctac
      901 gcgggaaggg tcggctccgg cctcgcgggg gagacggcgc agcgtgtgct gcgggagcgg
      961 ctgatcgagg tggatcggac gccgttcaca gtgcggctgc cgcgatccga cggcgtcggg
     1021 gctcactggt gcgagccgct gaccgtcgtc gaggtcgccc acaccggttg gaccgacggt
     1081 ggccgcctgc gccaaccggt gttccggggc gttcgcgatg atctggaacc cgagcaggta
     1141 cggcgggaag attgatccac tgcgggactg gccgatccgt ccggtgcggt gcaggatgtc
     1201 ccccgtcacc gctcgagaag ggggcgctgc accgtgcctg tgcctgtccg tatgcggcct
     1261 accacttcgg ttgggtgctg agtgtcaccc tcggccagcg gcgcacggcg tcgtctacgg
     1321 tggttccgat gacgacgatg ctcacctgga tggccgacga cggccacggg ctcgaaggtg
     1381 cgcgactgct cctccacacc tcgggcttcc gcgcgctggg caggatggtc cgggctgagc
     1441 cggggggtga cttcaccgcc tcctaccggc tgatcgtcgg cgaggacgct ggcctggagc
     1501 ggctctcggt gaccgcggcc accgcggagc gggaacgtca cctgacggtc aaccgtactg
     1561 acgacggggt gtggctgctc gacaccggga ccggcggtgg aagcacccgc accgacttcg
     1621 tgggcgcagt ggacgtcgac ctggagttca gcccgatgtt caacacgatc ccgattcgcc
     1681 gcctcggtct gcaccgcgag gcgggcgagc acacggtgcc gatggtgttc gtgtcgctgc
     1741 ccgatctcgg cgtgcgggtg gtcgagcaga cctaccgcac ggtgtcggag ctcgacgacg
     1801 ccaccgggca ggccgtggtg gagttccgca gcggagactt caccgcggag ctcgtggtcg
     1861 atgccgacgg tgcggtggtc aactacccgg ggctcgccag acggctcctg gccggcgtca
     1921 cgcctgcggc cggctgaggc tcagcccgcc cagcccacta ccgcgccacc gcggcggccg
     1981 atgtcccgca gggccgacag cgaggtccgg ccccgcggca cgcgcatgcc gtccgggttg
     2041 cggctggccg cccaccgctg ccgtccggca tgcccggcgt gcaccgtggc ggccagcccc
     2101 ccggtggagg cgagcgcacc gcgcatcgcg ccgagccggc ccagagcgtc gtccacggcg
     2161 gcgagcaggg cgtcgcgatt tccctcgcac atggcgagca ccagctccgg acgggtgccg
     2221 gccacccgcg tgccgtcggc gaacgaggat gcggcgagcg cgagcggcag gtcgtcctcg
     2281 cccgcggcgc cgacggcggc cagtacagcg gcgagcaggt gcggcaggtg tgacacccgg
     2341 gccacggcga ggtcgtgctc gtcggccgcc gccggcacga cccgcgcccc gcacgcccag
     2401 gcaagctcgg cgacgtcggc ccacacctcg aggtcgagcc cgtcgtcagc gaccacgacc
     2461 caggcggcgt cggcgaacag cccggcccgt cccgcgaccc agccggactc cgccgtgccg
     2521 gccatcgggt gcccgcccac gtaccgcgag cgtggggcgt accgggccac ggcgtcggcc
     2581 acggcgacct tgacgctcac cgcgtcggtc agccggcagg tcggggccgc cgcgtcgacg
     2641 cggcgaagca cctcgggtac gacagtgagc ggcacggcca gcaccacgag cgcgtcggac
     2701 tcggcagccc ggcgcagcgc cgcatcggtg tcggtggtga cgtcgaaccc atcggccctg
     2761 gcctgctccg cggtcgccgc cgatccgctc gtgccccaca cctcacgccc ggccttgtcc
     2821 gcggcgcgca gcagcgagcc gccgatcagg ccggtgccga tcacgcacac cgctcgctcc
     2881 gtcacaccac gcaggttatg cgcttcgcgc ccgtgcgcgg atatcggtgc cagagcactc
     2941 gtttccgcgc acgacccctc actcccgggc cagcgctccc gccacgcgca acactccccg
     3001 ctcctcgaac cgcacgccga cgacctgcac cccggccggc agcccgccat cggcgacgcg
     3061 gccgggcacc gacacggcag gccagccggt gaggttgaac ggcagcgtga ggtcgagcaa
     3121 ctgcggtcgc accggcacag ccacgccgtc gacatcgatc gcgtccacgc cgatggcggt
     3181 ggcgcgcagg cgggtggtgg gcagcaggag gacgtccacg tccgcgacgg cggcgcgcac
     3241 cgcggccgcg agccgtcgct tggcgcgcag tgcgtcgacg tactcgcgag caggctggtc
     3301 ggcccacagc aggagtcgtt ccctggtggc gtcctggtag tcctgcgggc gctcggccag
     3361 ccacgtcgag tgggtggcgt acgcctccgc cccgacgatc accgggtagg tggcggcgag
     3421 ctcgcccacc atcggggtgc tcacctcgac gacggaggcg ccgcgggcgc gcaacgtctc
     3481 ggccgcggcg tccaccgcgg cgttgagcgc cgggtcgaag gcccgccagt agtcgtcgac
     3541 cagcaccccg acccgcaccc ccgcgacgcc tggggcctgg atcccaccgc cggtgccgtc
     3601 cggcctgctg aggacatccc acgccaccga cgccgcgtgc acgtcggccg cgagcacccc
     3661 gacgtggtcg caggtctccg acagcgggaa caccccctct gtggacagcg cgccgaaagc
     3721 cggcttcagg ccgaccacac cgcacagggc ggccggcgtc cggacgctcg cgcccgtgtc
     3781 ggtgcccagc gcgagcggga ggtgcccggc cgcgatcgcg gctgctgacc ccgatgacga
     3841 gccacccgtg atccgactcg ggtcgtgcgg gttccgggcc gggccggtgg cggcgacgtc
     3901 gccggtcggg ccgtaggcga actcgtgggt gtgcagcttc gcgacgacga cggcgcccgc
     3961 cgcacggagc ctcgcgacga ccggggcgtc cgctgtggcc gccggggcgt ccgcgagcac
     4021 cctggagccc gccctggtgg gcagccccgc cacgtcgaac aggtccttca cgccgatcgc
     4081 gacgccatgg agcggccccc gccactcgcc gcgggcgagc tcggcggtca gcgcggccgc
     4141 ctgcgcgact gcccgctcgg cgtcgagcgt gatcacggcg ttgtgggagt cggtggcgag
     4201 gcgatcgagg acctcggtga cgtgctcgac gggggagagt tcgccggaac gcagcgtcag
     4261 tccgagatcg cgtagggagg tgatcacctg cccatcctga cgcagacacc ggcccggttc
     4321 ctcggacgtg tggcgagcag ctccaggaat gtgagaaacc cggcggcccg gccgcccccc
     4381 ggagcgtggc tcttggcggc agcctcgatg ccatgaccac cgcactcgtt gtgatcgacg
     4441 tccaacgcgg cttcgacgat ccctactggg gtccgcgcaa caatccggac gccgaggaga
     4501 acatcgccgc gctccttgac ggctggcggg ccacgggccg gcccgtcgtc ctcgtccacc
     4561 acgactcgct cacgccgggc tctccgctgg ccgccggcac acccggcaac ggcttcgcgg
     4621 ccatcctgga cggcgtccag cccgcgctga cgttcggcaa gaacgtcaac tccgcgttcc
     4681 acggcgagat cgacctcgac gcgtggctgc gcgaacacga catcgaggcc gtcgtgctgg
     4741 tggggatcca gacgaacatg tgcgtggaga ccacggccag ggtcggcggc aacctcggtc
     4801 accgcgtgac cgtcgccatc gacgccacct tcaccttcga cctcgccggt cccgacgggg
     4861 ccgtcctgac cgccgaggag ctcactcggg cgacggcggt caacctccac ggcggcgggt
     4921 tcgcgcggat cgcgacgacg acggaggtgc tggccgagct cggtcaggcg agtgcgtcga
     4981 gggccaaccc ggcgtagacg gcgacccctg cagccatggc ggcctcgtcg aagaccaccc
     5041 ggttggagtg gttgggtgcg gcagctcccg ggtggacgcc cggcgggcag ccgccgagga
     5101 aggccagcgc gcccggcacc tcggcgagca cgtaggagaa gtcctctgca cccatgatcg
     5161 gagtcggcat cgtgacgacc cggtccctgc cgaccacggt gtccgcgagg cggccgagtc
     5221 ggtcggcagc ctcgtcatcg ttgacggtga ccgggtagcc cggctcgatc tcgacctccg
     5281 cggtgcagcc gtgcgccatc gccgtgtagc ggcacacgcg ccggatctcg tcgtgcaccg
     5341 tggcccgtga cgcctcggag agggtgcgca cggtgccttc catgaaggcc gtctccggga
     5401 tgatgttggt ggtggtgccc gcaacgatcc gggtgatcgt cagcacgacg gggtggtggg
     5461 tgtcgacgcg gcgggtcacc gcggtctgca gcgctcccac catcgcggcg gccgccggca
     5521 gcgggtcgag cgcgtcgtgg ggggccgagg cgtggccacc gcgtccgctg acggtcaccc
     5581 ggatggtgtc cgccgccgcc atcagcgggc ccggccgcac gtgcacctcg ccggacggca
     5641 ccgtggcgct gatgtgcagc gcgtaggccg cgtcgggacg gccagcgggc ccggttcggt
     5701 ccagcagccc ctcgtcgatc atgtgccggg cgccgtggaa gccctcttca cccggctgga
     5761 acatgaacag gacccgcccg gccagctcgt cacgccgcgt cgccagcacc cgggccgccg
     5821 aggcgagcat ggccacgtgc gtgtcgtggc cgcaggcgtg catggcgccg ctcacctgcg
     5881 acgcgaaggc gagcccggtg tcctcggcga gcggcagtgc gtccatgtcg ccacgcagga
     5941 ggatcgtcgg cccggaccgg tgaccctcca gcaccgccac caccgagctg accgcgcgac
     6001 cgaggtgcac ctgcagcggg aggtccgcca gcgcggtgac gacggcgttg cgggtgcgcg
     6061 gcagctcgag cccgagctca ggatggcggt gcactgcgcg acgcaacgcc accgtccgtg
     6121 gttggacgcc gcgggcctgg gtgaggagtc cagtgatctg cgcagcatcc agtgacatgg
     6181 agcgatcctg gcagctctcc gaggtgacgt ggatggcatt tcgggttgtg gtttggacca
     6241 cagtgactcg gaggcctacc gtagtgggca tggcggtgca gagcgttgag gcgcgcgcgg
     6301 acgtgcgtga acaagccctg cccgggtacg ccgttgcggc gatccgggag gacgggcagt
     6361 ggcgctgcac ccgcatgacg cccgaggcgc tcgtcgacct cgacaccgct atcactgagc
     6421 tgcgcgagct acggtccacc ggagcggttc tcgggctgct cgacgtcgac gacgagttct
     6481 tcgtgctgct gcggcccagc ccgggcggga tcgcgctgat ggtgtccgac gccgtggcgg
     6541 ccctcgacta cgacgtggcc gccgacgtcc tcgacctgct gcgcctcgag ctcccgccgg
     6601 aggaggaggc tctcgactct ccgccgtggc ccgagggcga cctcgcgatc ctcgccgatc
     6661 tcggcctgcc cgccgacgag ctcgaggtac tcgccggcga ggtcgagctc taccccgacg
     6721 agcagctggc cgcgatcggt cgccgctgtg ggttcgccga cgcgctggcc gaggttgtcg
     6781 acgcagggtg accgccccgc ggggcgcagg cgcagcgcgc accgggtggg gccccacccc
     6841 atcggatgag ctgctggtcc gccgcgctat cgagattgct gctgccgcac cgcgcaccgg
     6901 tgacgtgccg atcggcgccg tggtcgtcga cgccgagggc cgggagctgg ctgcggcgtg
     6961 caacggccgc gaggcgctgg gcgacccgac ggctcacgcc gagctgctgg cattgcgggc
     7021 ggccgccgcg gtgcgggggg aatggcggct cgagggctgc acgctggccg tgacggtcga
     7081 gccctgcacg atgtgcgcgg gcgcgatcgg gctggctcgg gtcgcgcgag tggtgttcgg
     7141 ggcgtgggag ccgaagacag gagcggccgg ctcgctgtgg gatgtcctgc gcgaccgcag
     7201 gctcaaccac cggcccgagg tggtggccgg agtgctggcg caggagtgtg cggcgttgct
     7261 ggagtcgttc ttcgccgggt atcgatgagc gagcttgcga gcgacaaagc gccttcgcgc
     7321 cgctgaccga gcgcagcgag ggattgcgat gagcacgcgc tagcctgcgt cgatggagca
     7381 gttggtgcag cggacccgcg tcggcgcgta cgtggtgtgc gtgcaggacg acgcgctgct
     7441 gctggtccgc ttcagcggca gcgaccggtg gacgttgccc ggtggcgggc tcgaccacgg
     7501 cgagcggccc gaggacgccg cggtgcgcga ggtcgatgag gagacgggcc tgcaggtcgt
     7561 gctcggcccg ctgatcgggg tcgactccac gcgctgggac cgcgcgagcg acggcaggcc
     7621 gatcgacatg cacgccctgc ggatcctcta caccggcgag gtggtcggcg gctcgctgcg
     7681 tgacgagatc ggtgggagca ccgaccgggc cgagtgggtg cggctcgcgg tcgtcgccgc
     7741 ccgcccccgc gcccggctgg tcggcatcgg gctcgccgca gcaggccacg cctgaccgct
     7801 acacggccag cgtccgcagc ccgacggcct ccgcggcggc gatctgcctg cggtcgtagg
     7861 acagcatgac cccggcgtcc agccggaggg ctgcggcgac gtgcagggcg tcgaggctgc
     7921 gcaggtgtgg gcccggcagg aggcctgcct cccggaagag cgatcggtcg gtctcgagga
     7981 ggtcgaaccg ctcgagcacg gacgtgacgg cgacctgcgg gagcccgacg cgcacggcca
     8041 ggcggcgcag ctccgtctcc agcaccagac acgacaccgg cgcctcgggc agtgcgtcga
     8101 ggtgctcggc gagccgcgcc gaggcctcct cctccaggag cagcttcgcg gccgcggacg
     8161 tctccacgta gacgatcaac ggtcgccgcg gaggtcgtcg agatcgtcct gcagcggatg
     8221 gtccaggcgt actcgcggca ggtcggcgaa accgcccctg acaagcgccc gccggatcgg
     8281 cagtacggct ccgggccggc gcgggggcgg gatcagcacg gcgacgacct cgccgttgtt
     8341 cgtcaccgcg atcgtctcgc ctgcctgcac ctcgcgcagt actgcgctgc tgttgttgcg
     8401 cagttcgcgg tgcgggatcg tcctggccac gagctcaccc ccgtagcaac cgtagcactc
     8461 acgccaaccg gcccgccgcg atcacccggg tcaggaactg ccgggtccgc tcgtgcaccg
     8521 gagcgcccag cacctggccc ggcggcccgg cttccagcac gcgaccgccg tcgaggaagc
     8581 acacgcggtc cgacacctgc ttggcgaacc ccatctcgtg ggtggccatg aggatcgtca
     8641 tgccgccccc ggccagctcc cggacgatca tgagcacctc gccgaccagc tccggatcca
     8701 gggcggacgt gatctcgtcg aggagcagca gccgtggctg caccgccagc gcgcgagcga
     8761 tcgccacccg ctgctgctgc ccaccggaca gccggtccgg gtaggcaccg gcgaacgact
     8821 cgagcccgac ccggcgcagc aggtcgcgcg cccgctcgtc cgcctcggcg gccggcacgc
     8881 ggtgcaccac ccgcggggcg agcgccacat tctgcgccgc cgtcatgtgg gggaacaggt
     8941 tgaacgcctg gaacacgatc gccatccggc gttgggcgcc ggcgacgtcg gaccgtgggt
     9001 cggagacatc gacgccgtcg aggaggacct gcccgtcgtc gatctcctcc aacaggtcca
     9061 cgcaccgcag cagcgtcgac ttcccggagc ccgacgtgcc gatcaacgca accacctggt
     9121 gctcgtcgac ggtcaggtcg acgccgccga gcaccgtcgc cgcgccgtag cgcttgacca
     9181 gcccgcgcac ctcgagcacg ggggtcatga ccagccctgc cggcgggcgg cccgtgcggc
     9241 cagggtgtcg gcgacccggc cggtcgggat cgcgagcagc acgaacagca gcgccgcgac
     9301 cacgtacggg gtgaagttga acgtccgggc cgcctcgatc tgcgcggccc ggatcgcgtc
     9361 cacggcaccg agaaccgaga tcagcccgac gtccttctgc agtgcgacga agtcgttgag
     9421 cagcggtggc agcacccggc gcaccgcctg cggcagcacg accagccgca tcgactgccg
     9481 atgggtgagt cccagcgatc gtgccgcggc tcgttgcgac gggtgcaccg actcgatgcc
     9541 ggcgcggaac acctctgcca cgtacgcgga gtagacgagg atcaacgtgg cagtgccgag
     9601 cacggcggtg tcggtcggca tgccctgcag ccgcagcgcc ggcaccccga acccgatcag
     9661 gtacaacgcg atgagcagcg gcaccccgcg gaacaggtcg acgtagccgg tggccagcgc
     9721 ccgcaccggg aggaacaccg ggccgcgcag cgtgcgcagc acggcgatca ccaggcccag
     9781 caccaggatc ccgatctcgg cgaccacgag cacgcggatg ttgagccaga gaccctcgag
     9841 gatcgacggc agcgatgacc acgccacgtg cacgtcgaag aacgtgtcgc ggacccgcgg
     9901 ccagcccggt gagctcgcca ccccgagagc gaccgcccca gcgaacacca ctgtggacgc
     9961 gaacgcgacc agcgtcgagc gccgtgcgcg cgaacgccgg tacgccagcc gctcccgggc
    10021 gagcgggctg agctccgtcg cgacgggtgg ctccgtcacc gcagttccgg tgccgacccc
    10081 gccgtagcga gccactgctg ctccagggcg gcgagggtcc cggcggcccg cagccgctcg
    10141 acggcatcgg acacgcaccc cgtgagcggg ctgcccttgt ccagcaccgc gccgaactgc
    10201 tcgggcgtac cgatgccggc cggcaactgc ccgatgatct tcccgccgtc cagctccgcg
    10261 gaggtgatga agaacgccgt gggtaggtcc acgacgatcg cgtccacctg cccgttggtg
    10321 agggccagct tcgcgtcgtc gttggtgttg aagacggcag gctgtgacga gggcgcgagc
    10381 tgggagacga tcgcctcgta gctggtcgtg ccgacctgcg cgcccagccg cgcgtccttc
    10441 agcccggcga tggtcgtcgc gccggcgaac gggctggatc cgagggcgac gacggcctgc
    10501 ttgacgtcgt agtagggcgc cgagaagtcg acggcctgcc ggcgctcgtc ggtgatcgag
    10561 aactcggtga ggttcaggtc gaacgctttg ggcccgggct ggacggccgc attgaacggc
    10621 acccgggtcc aggtcaccgt gtcgcgcggg tagccgagct cgccggcgat ggcgtacgcc
    10681 acggcgctct cgaacccccg gcccgacgtc gggtcgtcgt ccaggtacca cggtgggtag
    10741 acgggctggt cggtgccgac ggtcagggtt cccgcggtgc gggtcgggag cgccccgggc
    10801 acgcaggacg ccgccgctgg tggggtgttc gtgtcggcag gagcgcaggc ggcggcgagg
    10861 acgaccacgg ccgccgcgag caggcgccgg accgggagcg ggggggtcac cgggagattt
    10921 tggcgtgcgg cacccccggc gtcgatgatc gccacactcg cgacgatctc tgagcgatgc
    10981 ccggacccgg gcgcgcaccc ccaccgcgcc cgagcccggg gccgccgggg gaggtcagtg
    11041 cgcctgctgc cggcccagcc gtgccgtcgt ctccgccacg tccatgccgt gcagccgcag
    11101 caggttctcg ccgaggatct tgcgcttcgc ctgatcggtg agctgcgggt agccgtagcc
    11161 gtcgcagagg tcctgcggaa tctggaagtc catgaacgcc ttgatggccc actgcgggtg
    11221 gaagatcggt gcctcggacc catagacgat cttgtcctcg ccgcaccaga acagcagctt
    11281 gccgatgatc tcggcgaaca tccgcggcgc ccggacaagg aagttgatcg tcgctgcgag
    11341 cgaggcgtgc aggttcgggt accggatgag ctgccagcag gtctcgtcga tgaacggcag
    11401 gccgacatgg aagatcacga agttgatgtc agggaagttg gcggcggcgc cgtccatgtc
    11461 ccaggtccgg gtgtgctcga tcggctgcgg gcccagcggt actcccttgt gcacgccgat
    11521 gaggttgata cccagctcct gcgccttctc gaagatcggg aacgcgatct tcgggtcgtc
    11581 catccgccag gggaacggtg agccgtagtc gtagcggacg ttgtagaact tgaacgcctt
    11641 ggcgccgaac tcctt
//
LOCUS       DASRSC010000173        15296 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3335927,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000173 DASRSC010000000
VERSION     DASRSC010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 15296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15296
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3335927"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VFE39_08645"
     CDS             <1..314
                     /locus_tag="VFE39_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258706.1"
                     /translation="HLRATKYAAPGTAPNTVGVVVLDPPPPGSLRTAAVTALELVAAD
                     PGGQRRGYRAEVGTATGPPGGSVVVDLATRYVVLAHQPDGRWLVAADSPATPDLPAAE
                     D"
     gene            408..2126
                     /locus_tag="VFE39_08650"
     CDS             408..2126
                     /locus_tag="VFE39_08650"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET6258707.1"
                     /translation="MSFAELEELIRKYLSTGIAVVVILVAPGTEAAPGGGLPAEPSIT
                     TIEPDGSAPAAAGGGTGRTSGSEGSDGNSAPAPAPDGGGEPSGAGGTTTGRSDDGSGA
                     DDRTGASGRDPERDTAFEETDLGTAGAGDMQADVRPESETGTGDTGRSADDSGRDNRG
                     SAGEVVAGDRPNGDKPRSGAGTDNSPNDPASADRNHAGDSDSGSGTRYDTRSDAGSNS
                     DTDADDDSESDSNAGSGTDDSSDTSDDTRSGSGSGSGSGTGTDDGNGSDSSGDPRSGS
                     SSDSARSKTGTDAGSSAGTTAAEQHGWGEPNREDDFAKGTGQWDIYDGPGHAGQGTRS
                     PSAVSVQDGLLTITGDSSGTTAGMAWNPGQKYGRWEGRVRAPEGDPSYNALLLLWPDA
                     EDFPVGGEIDFMEMMDPTREQTDIFIHYGEDNSQVNGKVEVDGTEWHNWAVEWAPDAI
                     TAYVDGEEWYRTTDTSIFPPGPMHLCIQLDWFPEGDTPQESVMQVDWVRQYSLDESGT
                     GSDSRKSRSEGNPVDSDTNDESDTNDDSDTNDDTDSGDDITGSDRQKDSSGPIRELLR
                     RMFSWR"
     gene            2292..3218
                     /locus_tag="VFE39_08655"
     CDS             2292..3218
                     /locus_tag="VFE39_08655"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HET6258708.1"
                     /translation="MSDTQQLALPDPPPSGPPPADRRRLVLIVVAITAAAALVAVLIA
                     VVGSGGEGRRGGSQTPAPAPAEGGRDTNDPIRPQGLSTLDPSARSDSEAAIRGNWPLL
                     THDEFDGTAVNTGTWDLYTGETTGGVGKHDPDNLSVTDGTMKLTSHGYSSAGMAWSDG
                     QKYGRWEVRARTQRGNGYGAVILLWPDAEDWPEGGEINFMEIPKGQRSETHFVLHYGE
                     DNSQVGTTVPGEFTQWHNYAVEWAPDHVAGFIDGQEIFRSSDPKTIPPRPMHLAIQQD
                     IGPYGKDWIPPLDNTTPDRVQLEVDWVRIYGV"
     gene            complement(3375..4853)
                     /locus_tag="VFE39_08660"
     CDS             complement(3375..4853)
                     /locus_tag="VFE39_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET6258709.1"
                     /translation="MGSVRLVSAIYGTPVIERLFRVSERGSTVGRELRGGLVTFVTMS
                     YIIVLNPLILGSFTPDGPDAKTDVLGNVLGVPQVAAVTALAAGVMTLLFGLIANYPFA
                     IATGLGINTLVAVTLAPLMSWPEAMGLVVVDGLIIVLLAATGFRTAVFNAVPPALKAA
                     IAVGIGLFIAFVGLVDAGFVRRIADAAGTTVPVGLGIGNSIASWPTFVFVVGLLITAV
                     LVARGVRAGILLGVVTSTVIAIIVESIVHVGPSAGTNPKGWALSVPTLPDSVVRLPDL
                     SLVGAVSFGAFVHLGLLTVVLLVFTLVLANFFDAMGTMTGLGQQAGLVDKDGQLPAVG
                     RALIVEGAGAVVGGAASASSNTVFVESAAGIAEGARTGLANMLTGVLFLLAMFFTPLY
                     SVVPIEAAAPELVIVGALLMRQITQIDFTEFRVALPAFLTIVVMPFTYSIANGIGAGF
                     ISYTVLAAATGRARAVHPLMWVVSVAFVGYFAVGPLRALFRG"
     gene            4854..5105
                     /locus_tag="VFE39_08665"
     CDS             4854..5105
                     /locus_tag="VFE39_08665"
                     /inference="COORDINATES: protein motif:HMM:NF022205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2530 domain-containing protein"
                     /protein_id="HET6258710.1"
                     /translation="MVDPPALSPRLSQVHTVAVVGTTLWLLGAAALLILWAVLGRHLD
                     IWFTTCVAGALIGGFGYGIFSWQRATARRGSRMAQQGLE"
     gene            5134..5871
                     /locus_tag="VFE39_08670"
     CDS             5134..5871
                     /locus_tag="VFE39_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6258711.1"
                     /translation="MSDRPTAVVTGASSGIGAATARALAKAGFYVVLGARRVDRCAEI
                     AAEIDGTAVRLDVTDAESVAAFAAAVPDCKLLVNNAGGAKGLEPVVQADEDAWRWMFE
                     TNVIGTLRVTKALLPALLASGDGHIVTVTSIAALESYDNGAGYTGAKHAQAMLHRTLR
                     GELLGQPVRLTEVLPGLVETEFSLVRFDGDEERAAGVYRGITPLTPADVADVIAFAAT
                     RPSNVNIDQIVIKPRNQASAMRAHREP"
     gene            complement(5883..6095)
                     /locus_tag="VFE39_08675"
     CDS             complement(5883..6095)
                     /locus_tag="VFE39_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258712.1"
                     /translation="MRPDELSEQTTTRAEPLPEERVAEHGGEDRRAEAAEVLRDSEER
                     LAEVVEGNVPGDAAHEHRRSEETATM"
     gene            complement(6133..7308)
                     /locus_tag="VFE39_08680"
     CDS             complement(6133..7308)
                     /locus_tag="VFE39_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET6258713.1"
                     /translation="MKRMLLVLAVVLVGTLGVVTAATAAGGLRGSAAASAPQVLAPSA
                     AIDYGPATGSSGTTPTGTATVTVRDGAFDDVTLTAPGGDTVQGTFNDDRTSWSTTEPL
                     GYDMSYTWGGTATGTDGMSVPLQGAFATLAPAKTVRGRLNIGDGRTVGIGAPVRIQFN
                     GHVADRAAVERALSVGTSVPVEGSWGWLPDEGGGSRVDYRPKAYWPSGTTVTVKANLY
                     GVAYGQGAYGAADISSTFEIGRAQIVKADVNSHRMIVLRDGHQVLDFPASYGLGSDPN
                     RNTRSGIHIVTEKFTDKRMISQRYGYDSVEQWAVRISNNGEFIHANPASAAAQGSANV
                     THGCVNLSIDNAKEYYDTALYGDPVEVTGTPVQLSARDGDIWDWTMSWDKWKGLSAL"
     gene            complement(7400..8437)
                     /locus_tag="VFE39_08685"
     CDS             complement(7400..8437)
                     /locus_tag="VFE39_08685"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET6258714.1"
                     /translation="MLVPDVVTRLAPLTTLRLGGPAGKVVAAASADIVVDAVRAADAA
                     GEPLLLLGGGSNVVIADEGWPGTVVRVASAGRVVERQSDGSVLLTVEAGEDWDDVVAA
                     CVADGLGGLECLSGIPGRTGATPVQNVGAYGVEIAELLIDVDLYDRRAGVVRQHVPAG
                     ELGLGYRASALKGRADAIVLRVRFALSGDSASAPIRYPELARALGVEPGSRVPAAVAR
                     EAVLGLRRGKGMVLDPADRDTWSAGSFFTNPVLPEAGVPGDAPRWPAGAGLVKIPAAW
                     LIERAGFRRGHPGPGGRVALSDKHVLALTNRGDGSTADLLALAREVRDGVRERFDVDL
                     VPEPVLIGCRI"
     gene            8462..8968
                     /locus_tag="VFE39_08690"
     CDS             8462..8968
                     /locus_tag="VFE39_08690"
                     /inference="COORDINATES: protein motif:HMM:NF022161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HET6258715.1"
                     /translation="MSHLVDHRSTSPYRADEVYAAMVDPEYLRARLAQIGGARPELLD
                     HHADEQGAHYRLRHGLDAKDLPSLLRGVLPGDITVERDESWTRVDRGRYTGETKVSVA
                     GTPASAAGGMRLNDTDGGGSELRVRIDVTVNVPVIGGRIESFVVGQVQSLLEMESRFL
                     QEWLAKTR"
     gene            complement(8856..10178)
                     /locus_tag="VFE39_08695"
     CDS             complement(8856..10178)
                     /locus_tag="VFE39_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6258716.1"
                     /translation="MTSRAIETDHPARTQRHAVAAMTFATGVIVANNYYAQPLEDALA
                     GAFHASSSAIGAVLTLIQLSYALGLATLVPLGDLLERRRLIVTMVSVTVIGMVLVAVA
                     PTLGVLAAAAAMVGLTTVAAQVLVPFAAHLAPKGQEGKMISTVMSGLLIGVLVSRVVA
                     GLVSQALGWRAVFILGALLTAVSVALLWRTLPVLPPATRVRYPALLRSVLTLLREEPV
                     LRWRIVYSASSYAAFGALWTSIGFMLAAPPHNFNEGSIGLFALFGVAGAVGARFAGRL
                     ADRGWAHYAVGGFLAVAVVAWVPISEGAGAGVGALVALAIGLLLFDLGVQGVHVSNQA
                     IIYALRPDARSRINSAYMTLYFLGGAAGSGLSAVLYAGHGWRGVSVLGVALPAVGLVL
                     WVVDTVLRRRAVSVSSRAIPAGTGTPSPTGSAPGRPRSSRSGRRSPAR"
     gene            10270..10604
                     /locus_tag="VFE39_08700"
                     /pseudo
     CDS             10270..10604
                     /locus_tag="VFE39_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030847.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
     gene            complement(10623..11051)
                     /locus_tag="VFE39_08705"
     CDS             complement(10623..11051)
                     /locus_tag="VFE39_08705"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03667 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HET6258717.1"
                     /translation="MTFGLPDTSTEFGARAARRLRDEVVAWLTTVDDAGTPQPAPVWF
                     LWDGESALIYSDSRAKRLRHIQYNPRVALHLDGDGTGGDIVVLTGEVAEAPDEPPVQN
                     NPAYLAKYGERITSGWGTPEVFASTYSVPLRFRPRRIRGH"
     gene            complement(11100..12497)
                     /locus_tag="VFE39_08710"
     CDS             complement(11100..12497)
                     /locus_tag="VFE39_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6258718.1"
                     /translation="MTQTVSGRVEALRAAMDGPVILPGDADYDDGRRVWNAGIDRRPG
                     AIARCASTADVVAAIGFAREEGLEVAVRGGAHNSSGTAVCDDGLMIDLSLLNQVVVDP
                     EGRRALVGGGALLTDLDSAAQAHGLAVPAGMIGHTGVGGLTLGGGMGWLTRKFGMSID
                     NLQSAEVVTADGRVLRASADEHPDLFWAVRGGGGNFGVVTSFEFRLHEVDPMVEFGLF
                     FWNLEQGPAVLRLARELIAALAPDLNIVIAALNAPPAPFVPAEHQFAPGYALLVTGFD
                     GTPAFAEIAARIRDDLPPLFDLVTPMPYVQLQQLLDEGNAWGQHCYEKGTAVAELTDA
                     VIDVVTEQVPLKNFPTSIVLFYWLDGAYSRVDDDATAFGGTRSPQFAVAIVGFAPDAD
                     LLVPDREWVRNFWTALRPLALGSGNGYVNTMSEYGDERLRASYGSAKYDRLAGIKTVY
                     DPGNFFHRNANIKPA"
     gene            complement(12627..14939)
                     /gene="lon"
                     /locus_tag="VFE39_08715"
     CDS             complement(12627..14939)
                     /gene="lon"
                     /locus_tag="VFE39_08715"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HET6258719.1"
                     /translation="MTETLKLPVLPLADSVVLPGMVVPVRLDEPEIQAAVDAANSVDR
                     KVLIVPRLDGKYAAVGTIAVLEQVGRLPSGERAAVVRGEGRAQIGSGVTGPGAALWVE
                     ASPIPEQPVTGRATELAKEYKALVIGILQQRGAWQVIDSVQQITAPGQLADTAGWASY
                     LDLDHKAQLLAETDVVKRLELLVEWTREHVAEQEISEKISNDVREGMEKTQREFLLRQ
                     QLAAIRKELGEDEPEGADDYRTRVENADLPEHVRKAALTEVGKLERASDQSPESGWIR
                     TWLDTVLDIPWTTKTDDTDDLVAARAILDADHAGLDDVKERLVEFLAIRARRNRRGLG
                     TVGGRGSGAVLSLVGPPGTGKTSLGESVARALGRKFVRVALGGVRDEAEIRGHRRTYV
                     GALPGRIVRAIREAGSMNPVVLLDEIDKLGSDFRGDPSSALLEVLDPAQNHTFRDHYL
                     EVDLDLSDVLFLATANVAETIPGPLADRMEMVTLDGYTEAEKVAIARDHLLPRQVEKV
                     GLEPSDVEFSEVALGMIAAEYTREAGVRQLERGLAKVLRKVAVQLDSDTAERPVHIDA
                     DALVGFLGRPRFTPESAERTSVPGVATGLAVTGAGGDVLFIEATSMEGEPGLTLTGQL
                     GDVMKESVSIALSYLRSRGLAGDLAARKLHVHVPAGAVPKDGPSAGITMVTALASLAS
                     GRPVKSSVGMTGEVTLQGKVLPIGGVKQKLLAAHRAGLTDVIIPKRNEPDLDDLPESV
                     RGQLRVHPVADVADVLELALEPATVAAAAA"
     gene            complement(15101..>15296)
                     /locus_tag="VFE39_08720"
     CDS             complement(15101..>15296)
                     /locus_tag="VFE39_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HET6258720.1"
                     /translation="ITGMEYGGITPVGLPEGWPLLVDAAVAAAPAVVIGSGVRHSKLA
                     LPGALAARLPGAEVADGLGR"
BASE COUNT         2456 a         5503 c         5257 g         2080 t
ORIGIN      
        1 cccacctgcg ggccacgaaa tatgcggcgc cgggcacggc gccgaacacc gtcggcgtgg
       61 tcgtgctgga tccgccgcca cccggatcgc tgcgtaccgc cgcggtcacg gcgctggagc
      121 tcgtcgcggc cgacccgggc ggtcagcggc gtggctaccg ggcagaggtc ggcactgcta
      181 cgggcccacc gggaggctcc gtcgtcgtcg atctcgccac gcgctacgtg gtgctggccc
      241 accaacccga cggccggtgg ctcgtggccg ccgactcccc tgccaccccc gatctccccg
      301 ccgcggagga ctgatgcgac aggagtgccc gtcatgagcg agcttgcgag cgaatcagtg
      361 tcacagcgcc ttcgcgcagc gccgaccgag cgtagcgagg gagtgcgatg agcttcgcgg
      421 agctcgagga gctgatccgg aagtacctgt cgaccggcat cgccgtggtc gtcatcctcg
      481 tcgcgcctgg caccgaggcg gcaccgggcg ggggccttcc cgccgagccg tcgatcacga
      541 cgatcgagcc cgacgggtcg gcaccggctg cggcaggcgg tggcaccggg cgcacctccg
      601 gcagcgaggg ctccgacggg aacagcgctc cggccccggc tcccgacgga ggcggcgagc
      661 cttccggcgc cggcgggacc accaccggcc gatcggacga tgggtccggc gccgatgacc
      721 gcaccggtgc gagtggccgg gatccggagc gtgacactgc cttcgaagag accgacctcg
      781 gaaccgccgg cgcgggagac atgcaggccg acgtgagacc agaatcggag accggcacgg
      841 gcgacaccgg ccgctcggcg gacgacagcg gccgggacaa ccgcggctcc gcaggcgagg
      901 tggttgccgg cgatcgcccg aacggcgaca aaccccgcag tggcgccggc acagacaaca
      961 gcccgaacga ccccgcctcg gccgaccgga accacgccgg agactccgat tccggctccg
     1021 gcaccagata cgacaccaga tccgacgccg gctccaacag cgataccgat gccgacgacg
     1081 actccgagtc cgactccaac gccggttccg gcaccgacga cagctccgac accagcgacg
     1141 acactcggtc cgggtccggg tccgggtccg ggtcgggcac aggtaccgac gacggcaacg
     1201 gctccgacag cagtggcgac cctcgctccg gctccagctc cgacagcgct cgtagcaaga
     1261 ccggaacgga tgcaggctcg agcgccggca ccacggccgc cgagcagcac gggtggggcg
     1321 agccgaaccg ggaggacgac ttcgccaaag gcaccggcca gtgggacatc tacgacggtc
     1381 ccgggcatgc ggggcagggc acccggtcgc cttctgctgt ctcggtgcag gacggtcttc
     1441 tcacgatcac cggcgattcc tccggcacca cggccggtat ggcgtggaat ccgggccaga
     1501 agtacgggcg gtgggagggc cgggtgcggg cgccggaggg cgacccgtcc tacaacgcgc
     1561 tgctgctgct ctggcccgac gcggaggatt tcccggtggg cggggagatc gacttcatgg
     1621 agatgatgga ccccacgcgc gagcagaccg acatcttcat ccactacggc gaggacaact
     1681 cgcaggtgaa cgggaaggtc gaggtcgacg gcacggagtg gcacaactgg gccgtggagt
     1741 gggcgccgga cgcgatcacc gcctacgtgg acggcgagga gtggtaccgc accaccgaca
     1801 ccagcatctt cccgcccggt ccgatgcacc tgtgcatcca gctcgactgg ttcccggagg
     1861 gcgacacgcc ccaggagtcg gtcatgcagg tcgactgggt gcggcagtac tccctcgacg
     1921 aatcgggcac aggttccgac agccggaaga gccggtcgga ggggaacccc gtcgacagcg
     1981 acaccaacga cgaaagcgac accaacgacg acagcgacac caacgacgac accgactccg
     2041 gtgacgacat caccggcagc gaccggcaga aggacagcag tgggccgatt cgggagctgc
     2101 tgcggcgcat gttctcgtgg cggtgacgcg cagtcatcgg catcgcgcat aggaggcgcg
     2161 gcggtcgacc ggccgcgatg gcagaggcga gctgcgccag ctccggccct ctggcgtgcc
     2221 gcaatcgatg aacgtaggtg atcgggaacc tctcagcgtg tacacagtgg cctcgcccta
     2281 cggttccgct cgtgtccgac acccagcagc tggctctgcc cgaccctccg ccttccgggc
     2341 ccccacccgc ggatcggcgt cggctggtcc tgatcgtggt ggcgatcacc gcggcggccg
     2401 ctctggtggc ggtgctgatc gcggtcgtcg gtagtggtgg cgaggggagg cggggcggga
     2461 gccagacacc ggcccccgcc ccggccgaag ggggccgtga cacgaacgac ccgatccgcc
     2521 cgcagggcct ctcgaccctc gacccgagcg cgcggtccga ctccgaggcc gcgatacggg
     2581 gcaactggcc gctcctgacg cacgacgagt tcgacggcac cgcggtgaat accggcacgt
     2641 gggacctcta caccggggag accaccgggg gcgtcggcaa gcacgacccg gacaacctgt
     2701 cggtcaccga cggcacgatg aagctcacca gccacggcta ctcctccgcc gggatggcgt
     2761 ggtcggacgg ccagaagtac ggacgctggg aggtccgggc acggacacag cgaggcaacg
     2821 gctacggggc ggtcatcctg ctctggccgg acgccgagga ctggccggag ggcggcgaga
     2881 tcaacttcat ggagatcccc aaggggcagc gcagcgagac ccacttcgtc ctgcactacg
     2941 gcgaggacaa cagccaggtc ggcacgaccg tgccgggtga gttcacgcag tggcacaact
     3001 acgcggtcga gtgggcaccc gaccacgtgg ccggcttcat cgacggccag gagatcttcc
     3061 ggtccagtga tccgaagacg atcccgccgc gtccgatgca cctcgcgatc cagcaggaca
     3121 tcggcccgta cggcaaggac tggatcccac cgctcgacaa cacgacaccc gatcgcgtgc
     3181 agctcgaggt cgactgggtg cgcatctacg gcgtctgaga ccggtcagtc cgcaccggaa
     3241 cacgcatcgg ggtcctggcc ggtcggtcag gaccccgatg cgctttccga actaacgcgt
     3301 cactacacca cgaagcaaca aaacgggagg tgagcctaac ctctcctgaa gtcgcaaggc
     3361 attcctccgc gccgctatcc gcggaacagc gcccggagcg gcccaaccgc gaagtagccg
     3421 acgaacgcca cggacaccac ccacatcagg gggtgcacgg cacgggcccg tccggtggcg
     3481 gcggccagca cggtgtagga gatgaatccc gccccgatcc cattggcgat cgagtacgtg
     3541 aaaggcatga cgacgatcgt caggaacgcc ggcagcgcga cccggaactc ggtgaagtcg
     3601 atctgggtga tctgccgcat cagcagcgcc cccacgatca ccagctccgg cgccgccgcc
     3661 tcgatcggca cgaccgagta gagcggggtg aagaacatcg cgagcaggaa gagcacgccg
     3721 gtgagcatgt tggccaggcc ggtgcgggca ccctcggcga tacccgccgc cgactcgacg
     3781 aacaccgtgt tggacgacgc cgacgccgcc cctccaacca ccgcgcccgc accctcgacg
     3841 atcagggcgc ggcccactgc gggcagctga ccatccttgt cgacgagccc ggcctgctgg
     3901 cccagcccgg tcatcgtgcc catcgcgtcg aagaagttgg cgagcacgag cgtgaacacc
     3961 agcagcacga cggtgagcag gccgaggtgg acgaaggcac cgaaggacac cgccccgacg
     4021 agcgaaaggt cgggcagcct gaccacgctg tcagggagag tcggcaccga cagtgcccat
     4081 cccttcgggt tcgtgcccgc cgacggtccg acgtgcacga tcgactcgac gatgatcgcg
     4141 atcaccgtgg aggtgacgac cccgagcaga atcccggccc gcacgccacg ggccaccaac
     4201 acggccgtga tcagcaggcc gacgacgaac acgaacgtcg gccacgacgc gatcgagttc
     4261 ccgatgccca gcccgaccgg gaccgtggtg cctgccgcgt cggcgatgcg ccggacgaat
     4321 ccggcgtcca ccagcccgac gaacgcgatg aacaggccga tcccaacggc gatcgccgcc
     4381 ttcaacgccg gcggaaccgc gttgaacacc gccgtgcgga agcccgttgc ggcgagcagc
     4441 acgatgatca gaccgtcgac cacgaccaga cccatcgcct ccggccagct catcaggggg
     4501 gcgagcgtga cggcgacgag cgtgttgatc ccgagaccgg tagcgatcgc gaacgggtag
     4561 ttggcgatga gcccgaacag cagcgtcatg acaccggccg ccagcgcggt caccgccgcg
     4621 acctggggca cgccgagcac gttgcccagc acgtcggtct tggcgtcggg tccgtccgga
     4681 gtgaagctgc cgaggatcaa cgggttgagc acgatgatgt aggacatcgt cacgaacgtg
     4741 acgagcccgc cgcgcagctc ccgaccaaca gtggagccgc gctcggacac gcggaagagg
     4801 cgctcgatca cgggggtacc gtaaatcgcg gacaccagcc gtacgctgcc catatggtcg
     4861 atcctccagc gctgtcaccg cggctcagcc aggtgcacac ggtggccgtc gtcggcacca
     4921 cactgtggct gctcggggct gcggctctgc tgatcctctg ggccgtgctt ggccgccacc
     4981 tcgacatctg gttcacaacc tgcgtcgccg gcgcgctgat cggcgggttc gggtacggca
     5041 tcttctcctg gcagcgtgca accgctcgcc gcggttcacg aatggcccag cagggcctgg
     5101 agtaaccttt ccgcgccgca ttaggctcga accatgagcg atcgacccac cgccgtcgtc
     5161 accggggcca gctccggcat cggcgccgca accgcccgtg cgctcgcgaa ggccgggttc
     5221 tacgtcgtgc tgggcgcccg gcgcgtcgac cggtgcgccg agatcgccgc cgagatcgac
     5281 ggcaccgcgg tgcgcttgga cgtcacggac gccgagtcgg tcgctgcctt cgccgcggcc
     5341 gtgcccgact gcaagctgct cgtcaacaac gccgggggag caaagggcct ggagccggtc
     5401 gtacaggccg acgaagatgc ctggcgctgg atgttcgaga ccaacgtgat cggcacgctg
     5461 cgcgtgacca aggcgctgct accggcgctg ctcgcctccg gcgacgggca catcgtcacg
     5521 gtcacctcga tcgcggcctt ggagagctac gacaacgggg cgggctacac gggggcgaag
     5581 cacgcgcagg cgatgctgca ccgcacgctg cggggggagc tgctcgggca gccggtgcgg
     5641 ttgacggagg tcctgcccgg cctggtggag accgagttct ccctcgtgcg cttcgacggc
     5701 gacgaggagc gggcggccgg cgtgtatcgc ggcatcacgc ccctgacccc cgcggatgtg
     5761 gccgacgtga tcgcgttcgc cgccacccgg ccgagcaacg tgaacatcga ccagatcgtg
     5821 atcaagcccc gcaaccaggc gagcgcgatg cgggcacacc gcgagccctg agccggcacc
     5881 tgctacatgg tcgccgtctc ctcggaccgg cggtgctcgt gggccgcatc gcccggcacg
     5941 ttgccctcga caacctccgc cagacgctcc tctgagtcgc gcaggacctc ggccgcctct
     6001 gcacggcggt cctccccacc gtgctcggca acccgctcct cggggagggg ctcggcccgg
     6061 gtggtcgtct gctcgctcag ctcgtcggga cgcatccggg cgcgtacccg gacgagccga
     6121 ggggcaaccc tcctacaggg cagacaggcc cttccacttg tcccagctca tcgtccagtc
     6181 ccagatgtcg ccgtcgcggg cagagagctg caccggcgtg cccgtcactt cgaccgggtc
     6241 gccgtacagc gcggtgtcgt agtactcctt ggcgttgtcg atcgagaggt tgacgcagcc
     6301 gtgggtgacg ttggcggagc cctgcgccgc cgccgacgcc gggttggcgt ggatgaactc
     6361 gccgttgttg ctgatccgca cggcccactg ctcgacgctg tcgtagccgt accgctggct
     6421 gatcatccgc ttgtcggtga acttctcggt cacgatgtga atgccggacc gggtgttgcg
     6481 gttggggtcg gacccgaggc cgtagctcgc cgggaagtcg aggacctggt gcccgtcgcg
     6541 caacacgatc atccggtgac tgttgacgtc ggccttgacg atctgtgcgc gaccgatctc
     6601 gaacgtcgag gagatgtcgg ctgcaccgta ggcaccctgc ccgtacgcca ccccgtacaa
     6661 gttcgccttg accgtgacgg tggttccgct cggccagtac gccttgggcc ggtagtcgac
     6721 ccgcgagccg ccgccttcgt cgggcaacca gccccacgag ccctcgacgg gcaccgacgt
     6781 gccgaccgac agggctcgct cgaccgcggc ccgatcggcg acgtggccgt tgaactggat
     6841 gcgcaccggc gccccgatac cgacggtgcg accgtcgccg atgttgagcc tgccacgcac
     6901 ggtcttcgcg ggcgccaatg tggcgaacgc gccctgcaac ggcacggaca tgccgtcggt
     6961 accggtggcg gtgccacccc acgtgtagga catgtcgtag ccgagcggct cggtcgtgct
     7021 ccagctcgtc cggtcgtcgt tgaacgtgcc ctgcaccgtg tctccgccag gggcggtcag
     7081 cgtgacgtcg tcgaacgcgc cgtcccggac cgtcaccgtc gccgttccgg tgggagtggt
     7141 gccgctcgag ccggtggccg gtccgtagtc gatcgccgcc gacggcgcca gtacctgtgg
     7201 cgccgaggcg gctgccgacc ctctcaaccc gcccgcggcc gtggcggcgg tgaccacacc
     7261 cagcgtgccc accagcacca ctgccaggac cagcagcatc ctcttcatga atccccccac
     7321 cgcactaccc gcagtgagag acgtccgagt acctgcccgg ttgccccccg tgactgcttg
     7381 gtgggagatt acgcgcgatt cagattcggc agccgatcaa caccggctcc ggtaccagat
     7441 cgacgtcgaa ccgttcgcgc accccgtcac gcacctcgcg ggccagcgcg agcagatcgg
     7501 ccgtggagcc gtcgccgcgg ttggtcaggg cgagcacgtg cttgtccgac agggcgacgc
     7561 gcccgccggg gccgggatgt ccgcgccgga acccggcccg ctcgatcagc caggccgccg
     7621 ggatcttgac gagcccggct ccggcgggcc agcgcggcgc gtcccccggt acgccggctt
     7681 ccggaagcac cgggttggtg aagaacgacc cggcgctcca ggtgtcgcgg tcggccggat
     7741 cgagcaccat gcccttgcca cgccgcagcc cgagaaccgc ctcccgagcc accgccgccg
     7801 gcacccggga gcccggctcg acgccgagcg cccgcgcaag ctcggggtag cggatcggcg
     7861 cgctcgcgct gtccccggag agggcgaacc gaacccgcag cacgatggcg tcggcgcggc
     7921 ccttgagtgc gctggcgcgg taccccagac ccagttcccc ggcaggcacg tgctgccgga
     7981 cgactcctgc gcgacggtcg tagaggtcga cgtcgatgag cagctcagcg atctccacgc
     8041 cgtaggcgcc gacgttctgt acgggggttg caccggtccg cccggggatg ccggagaggc
     8101 actccagccc cccgagcccg tcggccacgc aggccgcgac tacgtcgtcc cagtcctccc
     8161 ccgcctcgac cgtgagcagc acggaaccgt cgctctgccg ctccacgacc cgtccggcgc
     8221 tggccacccg cacgactgtg cccggccacc cctcgtccgc gatgacgacg ttcgagccgc
     8281 cgccgagcag cagcaggggc tcgccggcag cgtccgccgc ccggacggcg tcgaccacga
     8341 tgtcggcaga ggccgcagcg accaccttgc ccgcggggcc gccgagccgc agcgtggtca
     8401 acggggcgag gcgagtgacc acgtcgggta cgagcacggc gccaacggta acctccgaaa
     8461 catgtcccac ctggtcgacc accgctccac gtcgccgtac cgggcggacg aggtctacgc
     8521 ggcgatggtc gaccccgagt acctgcgggc ccggctggca cagatcggtg gggcacgccc
     8581 cgagctgctg gatcaccacg ccgacgagca gggcgcgcac taccggctgc gccacggcct
     8641 ggacgcgaag gacctgccgt ccctgctgcg gggcgttctt cccggtgaca tcaccgtcga
     8701 gcgggacgag agctggacgc gggtggaccg cggccgctac accggcgaga cgaaggtgag
     8761 cgtcgccggc actccggcgt ccgctgcagg cgggatgcgg ctcaacgaca ccgacggcgg
     8821 gggcagcgaa ctgcgtgtcc gcatcgacgt caccgtcaac gtgccggtga tcggcggccg
     8881 gatcgagagc ttcgtggtcg gccaggtgca gagcctgttg gagatggagt cccggttcct
     8941 gcaggaatgg ctcgcgaaga cacgctgacc gcacggcgtc gcaggacggt gtccaccacc
     9001 cagagcacga gcccgaccgc cggtagcgcc acgccgagca ccgacacgcc gcgccacccg
     9061 tggcccgcgt acagcacggc ggagagaccg cttcccgccg ctccgcccag gaagtacaac
     9121 gtcatgtatg cggagttgat ccggctgcgc gcgtccgggc gcagggcgta gatgatcgcc
     9181 tggttgctga cgtgcacgcc ctgcaccccg aggtcgaaca ggagcagccc gatcgcgagc
     9241 gccaccagcg ccccgacccc cgctccggct ccttcgctga tcggcaccca ggccaccacc
     9301 gcgactgcca ggaagccgcc gacggcgtag tgcgcccagc cgcgatcggc gagccgtcca
     9361 gcaaaccggg ctcccaccgc cccggccacc ccgaacaggg cgaacagtcc gatggatcct
     9421 tcgttgaagt tgtgcggagg ggcggccagc atgaagccga tgctggtcca gagcgcgccg
     9481 aacgcggcgt agctcgacgc gctgtagacg attcgccagc gcagcaccgg ctcctcgcgc
     9541 agcagcgtca gcacggagcg caggagcgcc gggtagcgca ccctggtggc gggtggcagc
     9601 acgggcagcg tgcgccacag cagcgcgacc gacaccgcgg tgagcagggc tccgaggatg
     9661 aacaccgcgc gccagcccag cgcctgcgac accaacccgg ccacgacgcg ggagaccagt
     9721 acgccgatca gcagcccgct catcaccgtg ctgatcatct tgccctcctg gcccttgggg
     9781 gcgaggtggg cggcgaaggg cacgagcacc tgcgcggcca cggtcgtgag cccgaccatg
     9841 gccgccgccg ccgcgaggac gcccagcgtc ggcgccacgg cgacgagcac catcccgatg
     9901 accgtcaccg acaccatcgt gacgatgagc ctgcggcgct ccagcaggtc gcccagcgga
     9961 acgagggtcg cgagcccgag cgcgtagctg agctggatga gtgtaaggac cgcgccgatc
    10021 gcggaggagg aagcgtggaa cgccccggcg agggcgtcct cgagcggctg agcgtagtag
    10081 ttgttggcga cgatcacacc ggtggcgaag gtcatcgcgg ccaccgcgtg gcgttgggtg
    10141 cgggccgggt ggtcggtctc gatcgcccgt gaagtcaccg ttcaagtctg cgcccggcga
    10201 ctcgcgctgg agacatgctc tttaccacag tgcaccatct cggaggtgga tgtcttcgcc
    10261 gagcagcgct atgtgtactc cgatccgacc accggctggt tcttcacgcg acaggtggtg
    10321 cgggccgact cactgccctt cgatgccgag gagctgcgga cccgcttctc ggaggtcgca
    10381 gcagagctcg gcggagaggc cgactggacg gtcaccgacg ccgcggtgcc caagcgcgct
    10441 gtcctgctgg tgaccaggga accgcactgc ctgcacgacc tgcgggtcgg gtcgcggccg
    10501 gggagctgcc tgtgcagctc gaggccgtga tcggcaacca cgactcactg ggcgacatcg
    10561 tgcgcgcgca cggcgtgccg ttccaccacg tcgcgttccc ggctccctcg accgcggcgt
    10621 cgtcagtggc cgcggatccg gcggggccgg aaccgcagcg gcaccgagta cgtggacgcg
    10681 aagacctcgg gggtccccca cccgctggtg atccgctcgc cgtacttggc gaggtaggcc
    10741 gggttgttct ggacgggagg ctcgtccggt gcctccgcca cctcaccggt gagcacgacg
    10801 atgtcgccgc cggtcccgtc cccgtccagg tgcagcgcca cccgagggtt gtactggatg
    10861 tgacgcagcc gcttggcgcg gctgtcgctg tagatcaacg ccgactcccc gtcccagagg
    10921 aaccagaccg gggcgggctg cggggtgccg gcatcgtcta cggtcgtgag ccaggcgacg
    10981 acctcgtccc gcaaccggcg ggcggccctc gccccgaact ccgtggacgt atcgggcagc
    11041 ccgaacgtca tggctcctcc tcagggcgac gacccgcctc gcagtcgcgg ggcgcgggat
    11101 caggcgggct tgatgttggc gttgcggtgg aagaagttgc cggggtcgta gaccgtcttg
    11161 atgccggcca gccggtcgta cttcgccgat ccatagcttg cccggagccg ttcgtccccg
    11221 tactcactca tcgtattgac gtacccgttc ccgctgccca gggctagcgg gcgcagcgcc
    11281 gtccagaagt tgcggaccca ttcgcggtcc ggcacaagca gatcggcatc cggcgcgaag
    11341 ccgacgatcg ccaccgcgaa ctggggtgat cgggtgcccc cgaacgccgt cgcatcgtcg
    11401 tcgacgcggc tgtaggcgcc gtcgagccag tagaacagca cgatcgacgt cgggaagttc
    11461 ttgagcggga cctgctcggt gacaacatcg atcaccgcgt ccgtcagctc cgcgacggcg
    11521 gtgcccttct cgtagcagtg ctggccccac gcgttgccct cgtcgagcag ctgctggagt
    11581 tggacgtagg gcatcggggt gaccaggtcg aacagcggcg ggagatcatc gcggatgcgg
    11641 gcggcgatct cggcgaacgc gggcgtcccg tcgaagccgg tgacgagcag cgcgtagccg
    11701 ggcgcgaact ggtgctcggc gggcacgaac ggagccggtg gcgcgttgag agcggcgatc
    11761 acgatgttga gatccggtgc gagcgccgcg atgagctctc tggccagccg cagaaccgcc
    11821 gggccctgct ccaggttcca gaagaacagg ccgaactcca ccatgggatc gacctcgtgc
    11881 agcctgaact cgaacgaggt gaccaccccg aagttgccac cgccgccgcg gaccgcccag
    11941 aacaggtcgg ggtgctcgtc cgcggaggcc cgcagcaccc gcccgtcggc cgtgacgacc
    12001 tccgccgact gcaggttgtc gatactcata ccgaacttgc gggtcagcca gcccatgccg
    12061 ccgccgagcg tgagcccgcc gacgcccgtg tggccgatca tcccggccgg aaccgccagt
    12121 ccgtgggcct gtgccgccga gtccaggtcg gtcaggagcg caccgccacc gaccagggca
    12181 cgccggcctt cggggtcgac cacgacctgg ttcaacaggc tcaggtcgat catcagaccg
    12241 tcgtcgcaca cggcggttcc cgacgagttg tgcgctccgc cgcgtaccgc tacctccagc
    12301 ccctcctcgc gcgcgaaccc gatcgcggcg acgacgtcgg cggtggaggc gcaccgggcg
    12361 atggcgcctg ggcgccggtc gatgcccgcg ttccagaccc ggcgtccgtc gtcgtagtcg
    12421 gcgtcgcccg gcaggatgac cgggccgtcc atcgctgccc gaagcgcctc gactctcccg
    12481 ctgaccgtct gagtcatgaa ctgttctcct cgttcgggac gtccgagtga cgtcgtccgg
    12541 tgaaggagca tggttaccga gcgcagatac ggagaacggg tggtcatgct gcggagcagc
    12601 acgaccaccc gttcggggac gatgactcag gcggcagccg ccgccaccgt cgccggctcc
    12661 agtgccagct ccaggacgtc ggccacgtcg gccaccgggt gcacccgcag ctgaccgcgc
    12721 accgactccg gcaggtcgtc gaggtcgggc tcgttgcgct tcggaatgat cacgtcggtc
    12781 agcccggctc ggtgcgcggc cagcaacttc tgcttgaccc cgccgatcgg gaggaccttg
    12841 ccctgcagcg tgacctcgcc cgtcatgccg acgctcgact tgaccggtcg cccggaggcg
    12901 agcgacgcga gcgccgtcac catcgtgata ccggccgagg ggccgtcctt cggaaccgcg
    12961 ccggccggca cgtggacgtg cagcttgcgc gcggccagat caccggccag cccgcgcgac
    13021 cgcaggtacg acagcgcgat cgacaccgat tccttcatga cgtcgcccag ctggcccgtc
    13081 agggtcagcc ccggctcgcc ctccatcgag gtggcctcga tgaacaggac gtcgccgcct
    13141 gcgccggtca cggcgagccc ggtcgcgacg cccggcaccg aggtgcgctc agccgactcg
    13201 ggggtgaacc gcgggcggcc caggaacccg acgagcgcgt cggcgtcgat gtgcaccgga
    13261 cgctccgccg tgtcgctgtc gagctgcacg gcgaccttgc ggaggacctt cgccaggccg
    13321 cgctccagct gccgcacgcc cgcctcgcgg gtgtactcgg cggcgatcat gcccagcgcg
    13381 acctcactga actcgacgtc ggacggctcc agcccgacct tctcgacctg ccggggcagc
    13441 aggtggtcac gcgcgatggc gaccttctcc gcctcggtgt acccgtcgag cgtcaccatc
    13501 tccatccggt cggccagcgg gccggggatg gtctcggcga cgttcgccgt ggccaggaac
    13561 agcacgtccg agaggtcgag gtcgacctcg aggtagtggt cccggaacgt gtggttctgc
    13621 gccgggtcga gcacctcgag cagcgccgag ctcgggtcac cccggaagtc gctgccgagc
    13681 ttgtcgatct cgtcgagcag caccaccggg ttcatcgagc ccgcctcgcg gatggcccgg
    13741 acgatccggc cgggcagcgc gccgacgtag gtgcgccggt ggccgcggat ctcggcctcg
    13801 tcgcgcacgc cgcccagtgc gacgcgcacg aacttgcgcc ccagcgcccg cgcgaccgac
    13861 tcgcccagcg acgtcttgcc tgtaccggga gggccgacca gggacagcac cgcaccggaa
    13921 ccgcgcccgc cgacggtgcc gaggccgcgg cggttgcgcc gcgcccggat ggcgaggaac
    13981 tcgaccagcc gctccttgac gtcgtcgagg cctgcgtggt cggcatccag gatggcccgg
    14041 gcggccacca ggtcatcggt gtcatcggtc ttcgtggtcc acgggatgtc gaggaccgtg
    14101 tcgagccacg tgcggatcca gcccgactcg gggctctggt ccgacgcgcg ctccagcttg
    14161 ccgacctcgg tcagcgcggc cttgcgcacg tgctcgggca ggtcggcatt ctccacacgg
    14221 gtgcgatagt cgtcggcacc ctcgggctcg tcctcgccca gctccttgcg gatcgcggcc
    14281 agctgctggc gcagcaggaa ctcccgctgg gtcttctcca tgccctcgcg gacgtcgttc
    14341 gagatcttct cggagatctc ctgctcggcg acgtgctcac gcgtccactc gacgagcagc
    14401 tccagccgct tgaccacatc ggtctcggcg agcagctgcg ccttgtggtc caggtcgagg
    14461 taggacgccc agcctgccgt gtcggcgagc tggccgggtg cggtgatctg ctgcaccgag
    14521 tcgatcacct gccaggcacc gcgctgctgc aggatcccga tgaccagggc cttgtactcc
    14581 ttggcgagct cggtcgccct gccggtgacc ggctgctcgg ggatcggact cgcctcgacc
    14641 cacagtgcgg cgccgggacc ggtcacgccg gacccgatct gtgcgcggcc ctcgccacgc
    14701 accacagccg cccgctcccc gctggggagc ctgccgacct gctcgaggac ggcgatcgtg
    14761 cccacggcgg cgtacttgcc gtcgaggcgc ggaacgatca ggaccttgcg atcgacgctg
    14821 ttcgctgcat cgaccgctgc ctggatctcc ggctcgtcga gccggacggg aaccaccatg
    14881 ccgggcagca ccaccgagtc ggcgagcgga agcaccggca gcttcagcgt ctctgtcata
    14941 ccctggtcaa cgcttacacc gttgacggaa ttccaaagct caagtaagtc cgccaggagc
    15001 gaacgcagaa accttgagcc ccctcggctc tactcggtga acgtcgaacg gcatctgcac
    15061 agggcgaaac gtaagcggcg ggctcgcgcg acccagcgtg ctaccggccg aggccgtcgg
    15121 ccacttccgc gccaggcaac cgcgcggcga gcgcgccggg tagggcgagc ttcgagtgcc
    15181 gcacgccgct cccgatcaca acggccggag cggcggccac ggcggcgtcg accagcagcg
    15241 gccagccctc gggcaggccg acgggggtga tccctccgta ctccatgccg gtgatc
//
LOCUS       DASRSC010000174        18826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3661908,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000174 DASRSC010000000
VERSION     DASRSC010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 18826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18826
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3661908"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VFE39_08725"
     CDS             <1..650
                     /locus_tag="VFE39_08725"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6258721.1"
                     /translation="AAVAIELAEARAEQQRAAMLDERDRIAADLHDHVIQRLFAAGLS
                     LRSIGTSLGSGPLADRVLATVQDLDATIGQIRTTIFQLHELPMSAPGGLQAQLLDVVA
                     EATPALGFEPAVRFPGAGAALPAEIAEDLVAVLREALTNVARHARARSAEVDLVARPD
                     RLVLTVRDDGAGMPPTMRSSGLANMRRRAERHRGSFHTARREPSGTVVTWSVPND"
     gene            884..1534
                     /locus_tag="VFE39_08730"
     CDS             884..1534
                     /locus_tag="VFE39_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6258722.1"
                     /translation="MTISVFLLDDHEIVRRGIAQLLESEDDIEIVGEAGTAAQAMARI
                     PALRPDVAVLDVRLPDGEGVSVCRDVRSAVTPPPACLILTSYSDDKALLGAIMAGAAG
                     YLLKQVTGVDLVGAVRTVAAGGSLLDPNATAAVLERLRKSGERADPRYESLSPQERRI
                     LGLIANGLTNRQIGTEMHLAEKTVKNYVSSLLHKLGFARRTEAAVYGAEMRRGDGR"
     gene            2606..3034
                     /locus_tag="VFE39_08735"
     CDS             2606..3034
                     /locus_tag="VFE39_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5313 family protein"
                     /protein_id="HET6258723.1"
                     /translation="MSRPHALPASPRHPGPLRWLWYAAGGRLPAAYREWVLYDITCRT
                     WPLRHLVRLLTQLAPVAVVLLVLIPGPVGVRVMAVLAGSLMGLAYSFVFLYEATEHRA
                     VKAGYPTGTAQRVREDRHAARALTRAADDFERSRRHLRGR"
     gene            complement(3049..3468)
                     /locus_tag="VFE39_08740"
     CDS             complement(3049..3468)
                     /locus_tag="VFE39_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET6258724.1"
                     /translation="MDLERALSFVRPRNKGVLTTLHKDGRPHLSNVLYATADDDSFWV
                     SVTEDRVKTRNMRRDPRVCLHVTDESFWQYVVLDGEARLGPVARDVGDEGVDALVEYY
                     RRASGEHPDWDGYRRVMVAEGRLIAVISATKAYGQVS"
     gene            complement(3550..4581)
                     /locus_tag="VFE39_08745"
     CDS             complement(3550..4581)
                     /locus_tag="VFE39_08745"
                     /EC_number="3.5.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF009803.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004328 - formamidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formamidase"
                     /protein_id="HET6258725.1"
                     /translation="MTGLGGLNPSPGGMVLGLVQAAVPIVTEPADLAAAVDRITGTVM
                     GARRALPAPELLVFPEYSLHGLDPSTWLDDRMLCDVDGPEVEKLRGACAEARIWGCFS
                     IMERNPGRAPYNTGIIVDDTGELRLHYRKLHPWVPAEPWHPGDLGIPVCDGPAGSRLA
                     LIICHDGMLPETAREAAYRGANVILRTAGYTYPIQQAWRFTNQGNAFCNLAYTASVCL
                     AGPDGAGIHSQGEAMVCDVDGTVLEQGDGTPGRIVTAEVVPARADEARRGWGVENNIY
                     QLGHRGYSAVEGGATDCPYTYVRDLAAGRYHLPWEGQVRHTDGTGAGLGTPADVRAAA
                     RPAAMPTDD"
     gene            complement(4578..6059)
                     /locus_tag="VFE39_08750"
     CDS             complement(4578..6059)
                     /locus_tag="VFE39_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET6258726.1"
                     /translation="MDAKQLCSLPATDLSRLVRSGEVSPVEVVDAALERIEHLDPHLH
                     AFVELTADRARQRAREIADALARGEDVGPLAGVPVAVKDLIATAGIPTRSGSRAYRDF
                     VPDEDDVVVERITAAGAVVLGKTTVPEFGYSGVGHNPISPTARNPWNSALTPGGSSAG
                     SGIAVATGMSPVALGSDGGGSVRIPAAHCGIYGFKASMGRVPLYPGCRDERYPGMSGW
                     ESLECIGPMTRTVDDAALMMSVLAGPDPRDRHSIPAGDVDWSTAAAGDVRGMRIAFSA
                     DLGYITVDPQVRAVVTDAVQAFRELGCTVEEVDPGWPDPHDTFWRLVVADTDLTGMRE
                     LAARYGNEMSPHLLDMLSRHWTAEQLTDAVMARKGVVNRMWRFMTDYDLLVTPTLTVP
                     PFPVGMQGPEEVDGRIVPSSAWLGFTQPINMTGQPAASCPAGMTADGLPVGLHIVGRH
                     LDDAAVLRASAAFERARPWAMRWPGMVEELTEAPMPTGAGVAP"
     gene            complement(6077..7417)
                     /locus_tag="VFE39_08755"
     CDS             complement(6077..7417)
                     /locus_tag="VFE39_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HET6258727.1"
                     /translation="MTTTRQTDPQVPEHHGLESEFEHEPVPVSRRRSLGSVSAVWFGF
                     PMIITNAVFGGTIVYGLGFWRGIAAIIIGNIVLLAYVGTLSYIAGRTGKNFALTAAET
                     FGRHGATIAAGFLSTVVIGWFAFQTGLTGSTLHASLGWNQTWMILLAGVLYIAITFVG
                     IRALTIIGLIAAPLFVVLAVVALVLAARNGGLSGITSYRGGGPNATVFSIGAAVTIVI
                     AGFADSGTMTADFTRWSRTGREAVIASFTAFPVANFISLLVGGIVVAAAKAVDPATNG
                     GDFLPILTSHGPVLSAIAVVFVFANLGSVCTHCLYNGAVGWSQLTGGKMRVLTIVLGL
                     LGLVAAVAGVWSYFSEWLNLLGIFVPPIGAILIVDQVLRRRAEARPVRPWRAAPFVAW
                     AIASAGSYAVHVMAPQLSEAIAGIVLGGVVYAVIDAFSPSPAREPASVPAADPA"
     gene            complement(7488..8279)
                     /locus_tag="VFE39_08760"
     CDS             complement(7488..8279)
                     /locus_tag="VFE39_08760"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HET6258728.1"
                     /translation="MSLAVPPGPAPVITRTLDLNCDLGESFGVYRYGADDAVMPLVTS
                     ANVACGAHAGDPRTMRTSVELAARHRVAVGAHVGLPDREGFGRRMIAVTAEEVHDLCL
                     AQMGALSAFTRAAGTPLVHLKLHGALYMMAMGDADLADAVCRAVLGFDASLRVVALPA
                     SALAASARAHGLTVVEEFFADRPYRNGQVTMFGWTPEELGPPDAAADRVEAMLADPTC
                     GSIGTVCVHSDTPGAPALLQAVSDRLRRLGCDLRAPAPASSAPRT"
     gene            complement(8276..9787)
                     /locus_tag="VFE39_08765"
     CDS             complement(8276..9787)
                     /locus_tag="VFE39_08765"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET6258729.1"
                     /translation="MPHEELTAAAFRQQLLAGTTSSADLVRWYLQRIADLDAAGPCLG
                     AVVTVNPDALADAEALDAAFAATGELRPLHGVPVLVKDQAETAGIPTAFGSALFTHYV
                     PERDATVVARLREAGAVILAKTAMCDFAAGWFSFSSRGEHTKNPYALDREIGGSSAGT
                     AAGLAANLGLLGVAEDTGGSVRVPGSFANLFGLRPTTGLVSRTGFSPLVHFQDTPGPM
                     GRTVGDVAALLDVMAGYDPADPFTAVAARSRGLGNYVTALAGADLSGARVGVLEDAFG
                     ARPESGPVNDAVRAAVEELRAAGAEVVDGLTLTGLQDWISQTSLYSTQSKEDITAFLA
                     ARPMAPVHSFDEIYASGTFHPLTDLMNDIAAGPQVAAEAPDYHRMRLRQEEFRRALLH
                     LMAGADVDVLVYPTVQVPPPTRQELADLRWTALTFPTNTVIASQSGMPAMTVPVGTTQ
                     DGLPVGMDVLGRPLTETHLLRFARAWEMHAQPRRAPVLPTAGAAPSALAEASR"
     gene            complement(9900..10616)
                     /locus_tag="VFE39_08770"
     CDS             complement(9900..10616)
                     /locus_tag="VFE39_08770"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6258730.1"
                     /translation="MNREAITVSTVNQEVYERLRGAIRTGSLPPGVKISLRSLADTLG
                     VSTMPVREAMRKLQAQGLVQFERRSVTVVQLTADEVVQVFQIRLRLEQLAAEWALQRL
                     TDEDIADLRAVLVEMADPGIAPEAWRRLNRQFHVRFYQCARSPHLLELIHNIWDRVDS
                     SMTIYASTVDDFAEAHRQHLRMLELIESRDLPGLLDETAWHLEYTSRTVAEALGEALG
                     EPVLHARADTADATPLTPHP"
     gene            10740..10901
                     /locus_tag="VFE39_08775"
     CDS             10740..10901
                     /locus_tag="VFE39_08775"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6258731.1"
                     /translation="MGRSPQYSMGGFPLSRPSRPDEVAELVAFLVSERASAITGAQHV
                     NAGGSLRTV"
     gene            complement(10995..11537)
                     /locus_tag="VFE39_08780"
     CDS             complement(10995..11537)
                     /locus_tag="VFE39_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HET6258732.1"
                     /translation="MTEQPSDTTAGSHGRVADKPWSPGSPTSPNRSARRTIASFDTYE
                     EAERAVDRLSDLDFPVERVAIVGQDLQTIEQVTGKLDYPRAAWRGAVSGAIPGVLIGW
                     IFGLFNWVDPLITGLLLALYGLVIGAVIGAIVGVIVYALQGGRRDFASVVMMRPQRFE
                     VVVDDEVAEQAAKLLGAAAP"
     gene            complement(11551..12186)
                     /locus_tag="VFE39_08785"
     CDS             complement(11551..12186)
                     /locus_tag="VFE39_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6258733.1"
                     /translation="MDLSGRTDQVPLHPADRIAASVALQRWVSTVHPSCRRNPVTLLR
                     FDPFRELDRLAEQTLSVGARALRTMPMEALRRGNQFLIYLDVPGVHPEDIDLTVERNV
                     VSVRARRAPARREGDEVIIDERPYCEFARQLFLGDNLDPDGMTADTANGVLTLTIPVS
                     EASKPRRVELGSSETGSSTGDATSGATGDRAGATTTTGSETASAADRPSTV"
     gene            12409..14112
                     /locus_tag="VFE39_08790"
     CDS             12409..14112
                     /locus_tag="VFE39_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6258734.1"
                     /translation="MYNGRPITAMREQLSNLCSLFTLSMVMSDGRDEWQILELAVTSV
                     GSLTSCRAVAGFLTGEGGELSSDSSTLAYPELTKRLVELDGADGRITGTGAAWAWAYA
                     LPTGHVHAGYYVVAADAEPPQSQLLLLRMLAQQTGRALATASLHRREREAAGQLRVLN
                     AQLADVNDRLAASVSDLERRRRVHETLSAVAASGAGEAGISHALHRLTGLGVAVEDPF
                     GNLRAWAGPGRPDPYPRLPARRRIELLADARRSPRPVRDRDRIIAVAQPRDEVLGVLA
                     LVDPGHQAGEHELFALEHATVVLSMELAHLRSLAENELRLRRDLMDDLLTGTDDESAL
                     SRSQALGHDLRTPHRVLVAHWTGAPTEDAVVRAVEQAVTRVLDTSVLLARRSGRVVLV
                     APHSNGQSDRNQWGELHREVTKRLRSTTGAIGVGGVCATPSEVPRSYAEAERALRVRL
                     GSSAPAGMTIYDDLGIYRLLVLGEDDHEVKQFVREWLGPLLDYDEASRSDLVTTLWQY
                     FECGGNYDNTARALLIHRSTLRYRLRRIRDLSGHDLGVVDSRLNLHVATRAWQILRGL
                     S"
     gene            complement(14141..14749)
                     /locus_tag="VFE39_08795"
     CDS             complement(14141..14749)
                     /locus_tag="VFE39_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET6258735.1"
                     /translation="MAHYFESPARGLAAGERAQVVPLRIGGREVRLATAPGVFSGSRV
                     DPGTAVLLDAVDFSGRHGDLLDIGCGYGPLSLTMASFAPDATVWAVDVNDRALELCRA
                     NAQRLGLNNVRVCRPEQVPAEIRFRFVCSNPAIRIGKPKLHAMLSHWLGRLEPDGEAY
                     LVVQRNLGSDSLHRWLVEQGWPTTRVTSVRGYRVLQCLPRNP"
     gene            complement(14811..15197)
                     /locus_tag="VFE39_08800"
     CDS             complement(14811..15197)
                     /locus_tag="VFE39_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HET6258736.1"
                     /translation="MEFHNVARSGQIPEGVVRRMFVGDVEIALSRSDGTVHATSNYCS
                     HLDCLLSSGKATAEGLLCSCHGSVFDYESGEPLCPPATRPITVFPVREDDGQIWVGVD
                     EELAKAAMRRRAARSRRRPALTPPDQ"
     gene            complement(15218..16474)
                     /locus_tag="VFE39_08805"
     CDS             complement(15218..16474)
                     /locus_tag="VFE39_08805"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6258737.1"
                     /translation="MSATPSTVVAVGAGQAAVSAVRMLRRRGFEGRLVVLGDERHAPY
                     QRPPLSKEYLRGEADLDDLTILQPSWCSANDVELLLGTRAERISYDTSARRVHLAGGG
                     EIVADAVLVATGVRARRLPGIEGNRVVHLRTLDDADRLRAQLPGAGRIAIIGGGLIGS
                     EVAASGRDLGVEVTCLEAAALPLLGQLGPAMACVYADLHRENGVDLRFGQQIESVVET
                     SSGVVVRTRLGDVVEADLLVVAVGAVPNDELARASGLAMDPVAGGVLVDAGCRTAIEG
                     VFAAGDIASRFDPTTGRHVRAEHLDNAGRQGMAVARALLGQAEVAEDPAWFWSDQYEH
                     NLQFVGRPRADATVVVRGSISDREFTAFYVHEGRVHAAFAVNSGGDVTAARQLITGAV
                     PVAETVLADEDTDLLALLDTVDTVDA"
     gene            complement(16528..16863)
                     /locus_tag="VFE39_08810"
     CDS             complement(16528..16863)
                     /locus_tag="VFE39_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET6258738.1"
                     /translation="MTPEAGTVEWVAVAPLVELQRRRKKQVEVAGRAIALFLVGDRVF
                     ALDDTCVHKGRSLSRGSLWKGKVVCPAHQWTFDPETGRADDQDDCQPVHDVLVVDGVV
                     HVDPRPRQI"
     gene            complement(16956..17714)
                     /locus_tag="VFE39_08815"
     CDS             complement(16956..17714)
                     /locus_tag="VFE39_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET6258739.1"
                     /translation="MTLTVDSSATTSDAPQLLSRGEFRAALENAIKGREAKNASFSQA
                     WADGLLTREHFARWAENHYHYVGPFADYLGYIYGNTPDRFTDAKDFTLQNMYEEELAD
                     IRHTDLLIRFGEACGTTKERIEDPNNMNAVTRGLQSWCYAVAMREHFVVATAALVVGL
                     ESQVPGIYKKQIVPLREAYGFTEDEIEFFDLHITSDEVHGERGYQIVLDHADTVELQQ
                     RCLDFVRWGAEMRFSYTRALYDTYVATTPAGAAG"
     gene            complement(17870..>18826)
                     /locus_tag="VFE39_08820"
     CDS             complement(17870..>18826)
                     /locus_tag="VFE39_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258740.1"
                     /translation="ISEKLGIAKSRPSVEIIAEPLTVVEDRVPVGAGVATRGGGGGEP
                     PVDVLPAAGGGGDAGDGPGGDSPEPAAAAADDEQDARTARLSRIFAIGVPVIFAVMVV
                     YMALGKFTTLVPPLAGGSAAGLVGGVAALLVIVAVVALDGPTKALSTCGEHLVDGLVF
                     AFKAMGVVLPIAGFFFLGNADYAAKIIGLPDGQKAPGLLVDLVTAGERFIPGNPLVMC
                     FGVLLVGMATGLEGSGFSGLPLTGSLSGALGHTVGMDPATLAAVGQMGSVWAGGGVLV
                     AWSSLMVVAGVTRVPVIDLARKCFVPVVAGLVVATLVAATMF"
BASE COUNT         2810 a         6673 c         6667 g         2676 t
ORIGIN      
        1 aggccgccgt cgccatcgag ctcgccgagg cccgtgcgga gcagcaacgc gccgccatgc
       61 tcgacgagcg cgaccgcatc gccgccgatc tgcacgacca cgtcatccag cgcctgttcg
      121 ccgccgggct gtccctgcgg agtatcggga cgagcctcgg gagcgggccc ctggccgatc
      181 gcgtgctcgc caccgtccag gacctcgacg ccaccatcgg ccagatccgc accacgatct
      241 tccagctcca cgagctgccc atgtcggccc ccggcggcct gcaggcccag ctgctcgacg
      301 tcgtcgccga ggccacgccg gcgctggggt tcgagcctgc ggtccggttc cccggtgccg
      361 gggccgccct gcccgccgag atcgcggagg acctcgtcgc cgtcctgcgc gaggcgctca
      421 cgaacgtcgc caggcacgcc cgtgcccgga gcgccgaggt cgacctcgtc gctcgccccg
      481 acaggctggt cctcaccgtt cgtgacgacg gcgccggcat gccgcccact atgcgtagca
      541 gtgggctggc caacatgcgc aggcgtgcag aacggcaccg cggctcgttc cacaccgctc
      601 gtcgggagcc gagcggcacc gtggtgacat ggtccgtccc gaacgactag cactcgtcca
      661 ccgacatgga ctgtcgggtc cgatgggggc cgagcgtgag ggcgtctcgg ggggctcccg
      721 gccgcacccg tccacgtcgt cgccgtcccc ggctgcgtct gtacggctga gacctgctgc
      781 atatcgccgc tcggactcct ccgttcgggc catcaggaga ggctgagttc gttgtgcgtc
      841 ggtaccgtcg acctgctcaa ccagcgacga gaggtccgat cccgtgacca tctccgtgtt
      901 cctgctcgac gatcatgaga tcgtccggcg cggcatcgcg cagctgctcg agtccgagga
      961 cgacatcgag atcgtcggcg aggcgggcac agcagcacag gctatggcgc gcatcccggc
     1021 cctgcggccc gacgtggcgg tgctcgatgt ccggctgcct gacggcgagg gggtgagcgt
     1081 gtgccgcgac gtccgctccg ccgtcacacc gccgcccgcg tgcctgatac tcacctcgta
     1141 ctccgacgac aaggcgctgc tcggagcgat catggcgggt gccgcaggat atctgctgaa
     1201 gcaggtcacg ggcgtagacc tcgtcggggc ggttcggact gtcgcagcag gcggctcgct
     1261 gctcgacccg aacgccaccg cggccgtgct ggagcggctc cgcaagagcg gcgaacgcgc
     1321 cgacccgcgg tacgagtcgc tcagcccgca ggagcggcgc atcctcggcc tcatcgccaa
     1381 cggactgacg aaccggcaga tcggcaccga gatgcacctc gcggagaaga cggtcaagaa
     1441 ctacgtgtcc tcgctgctgc acaagctcgg tttcgcccgc cgcaccgagg cggccgtcta
     1501 cggggcggag atgcgccgcg gcgacgggcg ttgacctggg cccggcagcg ggcgatgggg
     1561 caccatgcgc atggctcggg ggccgcacac cgtcgctccc cagcacggcc tacggccccc
     1621 ggccgtgccg aagggcgcgg gcccgatgac cgtccgtcac gatcgccctg gcccactggc
     1681 tccgcgccgc accggcccca accccgtcgc ggccgtgggc tgcagaacct catctcctcc
     1741 ggcctgtcca gaatcgcccg ctcgagccgg tcggggcaga ggcaaaggtc ccgttctcac
     1801 tgacgccacc ccctgcaacg cctcgccgtc gagtcgcatt tgaggctttg gcacgccctc
     1861 cgtgcctcag ctcggacgtt tccgacaaag cttctatttc ctccgccccg tgacccggag
     1921 ctgctggcgg aggtggcgac gtggctcgcc acgaatcttc gaggtcgaga ggcatcagcg
     1981 gagggagcct cggtaaattg actggacaca gactcgactt tctcgacaag tgtccgaatt
     2041 cggcaattgt ccgacaggtg gcggggcgat acacgtcgca ccacttcaat cggttttgat
     2101 ccgtcggcat cgcctcgtta gttcggtgcg agcggccagg gccagccgcc agcgccggtc
     2161 gagaggggtc gaggacgcga acgcagcgcc ggggcgcctc gccgtcccgc tcgtctgccg
     2221 ccaccacgtg gacctgtgcc gcaccacctg ctccccgcgc tcggtgtcac ccgcccgccg
     2281 cctgatcaca gcgccccggg caccatcgac tcgcgaccgc cgcgccccgc ctcgccacgg
     2341 cctggggagc ggccttcgag ccgctctccc cggagtagcg cgcgaccctc gtccgcacga
     2401 tccgggccta cgaggaggcg tccggctcct gacggccggt cgtgcgcgga tacgagtgct
     2461 ctagcactcg tatccgcgca cggggcgggc gtacgatccc gaccaccgga gacttcctgc
     2521 gtcggcaccg acgccgacgc cgcctctggc acacccccag tcccggtccc gggggccggg
     2581 acagtgcgcg aggggagcag tgtcgatgag ccgtccgcat gcactacctg cgtcgccgcg
     2641 acacccgggg cctctccgct ggttgtggta cgccgccggc ggtcggctcc ccgcggcata
     2701 ccgggaatgg gtgctgtacg acatcacgtg tcgcacgtgg ccgctacgcc acctcgtgcg
     2761 gttgctcacc caactcgcgc cggttgccgt cgttctgctg gtgctgatcc cgggcccggt
     2821 gggggtccgg gtgatggcgg tgctggccgg gtcgctgatg ggcctcgcgt actccttcgt
     2881 cttcctctac gaggccaccg agcaccgggc cgtcaaggcc ggctacccga ccggcaccgc
     2941 acagagggtt cgggaggatc gccatgcggc gcgcgcgctg acccgggctg ccgatgactt
     3001 cgaacggagt cggcgccatc ttcggggtcg gtgatcgggt gcgggccgtc aggacacctg
     3061 gccgtatgcc ttcgtggccg agatgaccgc tatcaggcgc ccctcagcga ccatgacccg
     3121 ccggtacccg tcccagtccg ggtgctcgcc cgaggcccgg cggtagtact cgaccagcgc
     3181 gtcgacgccc tcgtcgccga cgtcgcgggc aaccggcccg agccgggcct cgccgtccag
     3241 gaccacgtac tgccagaacg actcgtccgt gacgtgcagg cagacgcgcg ggtcgcgacg
     3301 catgttcctg gtcttcaccc gatcctcggt gaccgagacc cagaagctgt cgtcgtccgc
     3361 ggtcgcgtac aggacgttcg acaggtgcgg ccggccgtcc ttgtgcaggg tggtcagaac
     3421 gcccttgttg cgcgggcgaa cgaacgacag ggcgcgctcg agatccatcc ctcgatcctc
     3481 ccaggtccgg gcggtcgggc ggcgtcgagt caggtggcgg acgccctgac ccgaccgccg
     3541 tgccgcccgt cagtcgtccg tcggcatcgc cgccggacgc gcggctgcac ggacgtcagc
     3601 gggagttccg aggcccgcgc cggtgccgtc ggtgtggcgc acctgccctt cccacggcag
     3661 gtggtagcgc ccggctgcca ggtcccgcac gtaggtgtac gggcagtcgg tggcgccgcc
     3721 ctcgacggcg ctgtagccgc ggtgtcccag ctggtagatg ttgttctcca cgccccagcc
     3781 gcgccgggcc tcgtcggcac gcgccggcac cacctccgcc gtgacgatgc ggccaggcgt
     3841 cccgtcaccc tgctccagca cggtgccgtc cacatcgcag accatggcct cgccctgcga
     3901 gtggatcccc gccccgtccg gccccgcgag gcagaccgac gccgtgtagg cgaggttgca
     3961 gaacgcgttg ccctggttgg tgaagcgcca cgcctgctgg atcggatagg tgtagccggc
     4021 ggtgcgcagg atgacgttcg ccccgcggta cgcggcctcg cgcgcggtct cgggcagcat
     4081 cccgtcgtgg cagatgatga gggccagccg ggaaccggcg gggccgtcgc acaccgggat
     4141 cccgaggtcg ccggggtgcc acggctcggc cggcacccac gggtgcagct tgcggtagtg
     4201 cagccgcagc tcgccggtgt cgtcgacgat gatgccggtg ttgtacgggg cccggcccgg
     4261 gttgcgctcc atgatcgaga agcaccccca gatccgcgcc tccgcacacg ccccgcgcag
     4321 cttctcgacc tccgggccgt cgacgtcgca gagcatccgg tcgtcgagcc acgtcgaggg
     4381 gtccagcccg tgcaggctgt actcggggaa caccagcagc tcaggcgccg ggagggcgcg
     4441 gcgtgcgccc atcaccgtgc cggtgatccg gtccaccgca gccgccaggt ccgccggttc
     4501 cgtgacgatc ggcacggcgg cctggacgag gcccagcacc atcccgccgg gtgaggggtt
     4561 cagtccaccc aatcccgtca cggcgccacc cccgccccgg tcggcatcgg cgcctccgtc
     4621 agctcctcca ccatcccggg ccaccgcatc gcccagggac gggcccgctc gaaggccgcc
     4681 gaggcgcgca gcaccgccgc gtcgtcgagg tggcggccga cgatgtgcag gccgaccggc
     4741 agaccgtcgg ccgtcattcc cgcggggcag ctcgcggcgg gctgaccggt catgttgatc
     4801 ggctgggtga accccagcca cgccgacgac ggcacgatgc ggccgtcgac ctcctccggg
     4861 ccctgcatcc cgacggggaa cgggggcacc gtgagcgtgg gggtgaccag caggtcgtag
     4921 tcggtcatga accgccacat ccggttgacc accccctttc gcgccatcac ggcatccgtg
     4981 agctgctccg cggtccagtg gcgggagagc atgtcgagca ggtgaggcga catctcgttg
     5041 ccgtaccggg ccgccagctc gcgcatcccg gtcaggtcgg tgtccgcgac gaccaggcgc
     5101 cagaacgtgt cgtgcgggtc cggccagccg gggtcgacct cctcgaccgt gcacccgagc
     5161 tcgcggaacg cctgcacggc gtccgtcacg acggctcgca cctgcggatc gaccgtgatg
     5221 tagcccaggt ccgcgctgaa cgcgatgcgc atcccgcgga cgtcacccgc cgctgcggtg
     5281 ctccagtcga cgtcaccggc cgggatcgag tggcggtctc gggggtcggg gccggcgagc
     5341 accgacatca tcagtgcggc gtcgtcgacc gtgcgggtca tcgggccgat gcactcgagc
     5401 gactcccagc cggacatccc cggatagcgc tcgtcgcggc agccgggata gagcggcacc
     5461 cgccccatcg atgccttgaa cccgtagatg ccgcagtggg cggccgggat gcggacggag
     5521 ccgccgccgt cgctgcccag cgcgacaggg gacatgcccg tcgccaccgc gatcccggac
     5581 cccgcgctcg acccacccgg tgtgagggcg gagttccagg ggttacgggc cgtcggggag
     5641 atgggattgt gtcccacacc gctgtagccg aactccggca cggtcgtctt tcccagcacc
     5701 acggcgccgg ccgcggtgat ccgttcgacc accacgtcgt cctcatcggg cacgaagtcg
     5761 cggtaggcgc gcgaccccga gcgggtcggg atccccgccg tcgcgatgag gtccttcacg
     5821 gccacgggca cgcctgccag cggacccacg tcctcccccc gcgccagcgc gtcggcgatc
     5881 tcccgcgccc gctgccgggc gcggtcggcg gtgagctcga cgaaggcgtg caggtgcggg
     5941 tccaggtgct cgatgcgctc gagcgccgcg tcgaccacct cgaccggtga cacctccccg
     6001 ctgcggacca gccgggacag gtcggtagcc ggtagcgagc acagctgctt cgcgtccacg
     6061 atgtcctcct ccgcgctcag gccgggtcag cggccgggac cgacgcgggc tcccgcgcag
     6121 ggctggggct gaacgcgtcg atcaccgcgt agacgacgcc gccgagcacg atgccggcga
     6181 tggcctcgct gagttgcggc gccatgacgt ggaccgcgta cgagccggcc gaggcgatcg
     6241 cccaggccac gaacggggct gcgcgccacg gccgcacggg gcgggcttcg gcgcgccggc
     6301 gcagcacctg gtcgacgatg aggatggccc cgatcggcgg gacgaagatt ccgagcaggt
     6361 tcagccactc ggagaagtag ctccacactc ccgccacggc ggcgacgagc cccaggagcc
     6421 cgaggacgat cgtgagcacg cgcatctttc caccggtcag ctggctccaa ccgacggcgc
     6481 cgttgtagag gcagtgggtg cagaccgagc cgaggttcgc gaagacgaac acgaccgcga
     6541 tggcggagag caccggaccg tggctggtga ggatcggcag gaagtcacca ccgttggtgg
     6601 ccggatccac ggccttggcc gcggccacga cgatgccgcc gacgagcaac gagatgaagt
     6661 tggcgacggg gaatgccgtg aaggaggcga tgaccgcctc acgcccggtc cgcgaccagc
     6721 gcgtgaagtc ggcggtcatc gtgccggagt cggcgaagcc tgcgatgacg atcgtgacgg
     6781 cggcgccgat gctgaagacc gtggcattcg ggccgccgcc cctgtagctg gtgatccccg
     6841 acagtccgcc gttgcgagcg gcaagcacga gcgcgaccac cgcgagcacc acgaacagcg
     6901 gggcggcgat gaggccgatg atcgtgagcg ctcggatgcc gacgaacgtg atggcgatgt
     6961 agagcacccc ggccagcagg atcatccacg tctggttcca gccgagcgac gcgtgcaggg
     7021 tcgacccggt gaggccggtc tggaatgcga accagccgat gacgacggtg gagaggaagc
     7081 ccgccgcgat cgtcgcgccg tggcggccaa acgtctcggc ggcggtgagc gcgaagttct
     7141 tgccggtccg gcccgcgatg tagctcaagg taccgacgta ggcgagcagg acgatgttgc
     7201 cgatgatgat ggccgcgatc ccgcgccaga aaccgaggcc gtagacgatg gtgccgccga
     7261 agaccgcgtt ggtgatgatc atcgggaagc cgaaccagac ggccgacacg gagccgagcg
     7321 atcgcctccg cgagaccggc acgggttcgt gctcgaactc cgactcgagc ccgtggtgtt
     7381 cagggacctg cgggtccgtc tgccttgtgg tggtcacgac gtggtccttc cgatcgggtg
     7441 agccagtacg tccgtggacc ggcggggacg aagaggtgtg gctgccgtca ggtacgtggt
     7501 gcggaagaag cgggcgccgg agcgcgcagg tcgcagccga gccggcgcag gcgatcgctg
     7561 acggcctgca ggagggcggg cgcgccaggg gtgtcgctgt ggacgcagac ggtgccgatg
     7621 gagccgcagg tggggtcggc gagcatggcc tcgacccggt cggccgcggc atccggagga
     7681 ccgagctcct ccggcgtcca gccgaacatc gtcacctggc cgttccggta cgggcggtcg
     7741 gcgaagaact cctcgacgac ggtcaggccg tgggcccgag cggacgcggc gagggcggac
     7801 gcgggcaggg cgaccacccg caggctcgcg tcgaagccga ggacggcccg gcacaccgcg
     7861 tcggccaggt ccgcgtcgcc catcgccatc atgtacaggg caccgtgcaa cttcaggtgc
     7921 acgagtgggg tcccggccgc ccgggtgaag gcggacaggg cacccatctg cgcgagacag
     7981 aggtcgtgga cctcctccgc cgtcacggcg atcatgcggc gcccgaagcc ctcccgatcg
     8041 gggagcccca cgtgcgcacc gacggcgacg cggtgccggg cggcgagctc gacgctcgtg
     8101 cgcatggtgc gcggatcgcc ggcatgggcg ccgcaggcga cgttggccga ggtgacgagc
     8161 ggcatcaccg cgtcgtcggc gccgtaacgg tagacgccga aactctcgcc gaggtcgcag
     8221 ttgaggtcca gcgtcctggt gatgaccggt gccggtccgg gagggacggc gaggctcacc
     8281 gggacgcctc ggccagcgcg gaaggagcag cacccgcagt gggcagcacc ggcgcacgcc
     8341 gcggttgcgc gtgcatctcc caggcgcggg cgaacctcag cagatgcgtc tccgtgagtg
     8401 ggcggccgag gacgtccatg ccgaccggca ggccgtcctg cgtcgtcccg acgggcacgg
     8461 tcatggccgg catgccgctc tgcgaggcga tgacggtgtt cgtcgggaac gtgagtgcgg
     8521 tccagcgcag gtccgccagc tcctgtctcg ttggcggcgg tacctgcacg gtgggataga
     8581 cgaggacgtc gacgtcggca ccggccatca ggtggagcag ggcccggcgg aactcctcct
     8641 gccgcagccg catccggtgg tagtcgggag cctcggccgc cacctgcggt cccgcggcga
     8701 tgtcgttcat caggtcggtc agcgggtgga acgtcccgct ggcgtagatc tcgtcgaagc
     8761 tgtgcaccgg cgccatcgga cgggccgcga ggaaggccgt gatgtcctcc ttcgactggg
     8821 tggaatacag cgaggtctgg ctgatccagt cctgcagacc tgtcagcgtc aggccgtcga
     8881 caacctcggc gccggcggcc cggagctcct cgaccgcagc gcgcacggcg tcgttgaccg
     8941 gaccggactc gggccgcgcc ccgaacgcgt cctcaaggac gccgacgcgc gcgccggaca
     9001 ggtcggcgcc ggcaagcgcg gtgacgtagt tgcccagccc gcgcgaccgt gcggccaccg
     9061 cggtgaaggg gtcggcgggg tcgtacccgg ccatgacgtc caggagcgcg gcgacgtcgc
     9121 cgacggtgcg gcccatcggc cccggcgtgt cctggaagtg cacgagcggc gagaagccgg
     9181 tgcggctcac cagcccggtt gtggggcgca gcccgaacag gttggcgaag gacccgggca
     9241 cccgaacgga cccgcccgtg tcctcggcga ccccgaggag gcccaggttc gcggccaggc
     9301 ccgctgcggt tccggcgctg gagccgccga tctcgcggtc gagggcgtac gggttcttcg
     9361 tgtgctcgcc tcgtgaggag aacgagaacc aaccggcagc gaagtcgcac atcgcggtct
     9421 tggcgaggat caccgccccc gcctcccgca ggcgggccac caccgtcgcg tcccgctccg
     9481 gcacgtagtg ggtgaagagc gccgatccga acgccgtcgg gatgcctgcc gtctcggcct
     9541 ggtccttgac gaggacgggg acaccgtgca gcggacggag ctcccccgtc gccgcgaacg
     9601 cggcgtcgag ggcttcggcg tcggcgagcg cgtcggggtt gaccgtcacg acggcaccca
     9661 ggcacgggcc ggcggcgtcg aggtcggcga tgcgctgcag gtaccagcgc accaggtcgg
     9721 cgctgctcgt ggtgccggcc agcagctgct gacggaaagc ggcggccgtg agttcctcat
     9781 ggggcacgtg gttctccctc aggtccgggg cgcgcgggag cgcaccgggt cgcaatgcgt
     9841 ccgggcgggc ggccctcgtg tgtgcgaccg gtgcggtcgc gccaaatcag ggtgtggggt
     9901 caggggtggg gggtcagggg tgtcgcgtct gcggtgtcgg cgcgcgcgtg cagcacgggc
     9961 tcaccgagcg cctcccccag cgcctcggcg acggtgcggc tggtgtactc gaggtgccag
    10021 gccgtctcat cgaggaggcc gggcaggtcg cgggactcga tgagctcgag catcctcaga
    10081 tgctggcggt gcgcctcggc gaagtcgtcc accgtcgagg cgtagatggt catcgaggaa
    10141 tcgacccgat cccagatgtt gtggatgagc tcgagcagat gggggctgcg ggcacactgg
    10201 tagaaccgca cgtggaactg gcggttcagc cgccgccatg cctccggcgc gatgcccggg
    10261 tcggccatct ccacgaggac ggcgcgcagg tccgcgatgt cctcatcggt gaggcgctgt
    10321 agggcccact cggcggccag ctgttccagc cgcaggcgga tctggaagac ctggacgacc
    10381 tcgtccgccg tcagctggac gaccgtgacg ctgcggcgct cgaactggac gaggccctgc
    10441 gcctgcagct tccgcattgc ctcccgcacg ggcatcgtgc tgacgcccaa cgtgtcggcc
    10501 agcgaccgca gcgagatctt gacccccggc gggagggacc ccgtacggat cgcgccccgc
    10561 agccgctcgt acacctcctg gttgaccgtc gagaccgtga tcgcctcccg gttcacggga
    10621 tacctcctgc tgtgatctgt gatcacagac gctacggagg ccatgtgtcc gcgccgtaga
    10681 cgcgcgttgc gactgcatta cgagtgccgc tcgctcgagt cggggagccg aaacacgtcg
    10741 tgggccgctc cccgcagtac tccatgggcg gattcccact cagccgaccg agccggcccg
    10801 acgaggtcgc cgaactggtc gcgttcctcg tctccgagcg tgcgtccgcg atcaccggcg
    10861 cccagcacgt gaacgccggc ggcagcctcc ggaccgtctg accgccgccg tctcacacgt
    10921 gcgcagccag gcgtctcagc aagcgcgtgc ccatcacccg tgacgcggcg ctcgccgacc
    10981 taccgccgag ccactcaggg cgccgccgct cccagcagct tcgccgcctg ctcggccacc
    11041 tcgtcgtcga cgaccacctc gaagcgctgc ggccgcatca tcacgaccga ggcgaagtcg
    11101 cgacggccgc cctgcagcgc gtagacgatc accccgacga tggcgccgat caccgcgccg
    11161 atgaccaggc cgtagagggc gagcagcaag cccgtgatca gcgggtcgac ccagttgaag
    11221 agcccgaaga tccacccgat cagcacgccg ggaatggcac cgctcaccgc tccccgccat
    11281 gcggcgcgtg gatagtccaa cttcccggtg acctgctcga tggtctgcag gtcctgcccg
    11341 acgatcgcca cccgctcgac cgggaaatcg aggtcggaga gccggtcgac ggcccgctcc
    11401 gcctcctcgt aggtgtcgaa ggaggcgatc gtccgccggg ccgaccggtt cgggctcgtc
    11461 gggctcccgg gcgaccacgg cttgtctgcg acacgtccgt gcgatccggc cgtggtgtcg
    11521 ctgggctgtt cggtcatgat gtctccccga tcagacggtc gacggccggt cggccgccga
    11581 agcggtctcc gacccggtcg tggtggtggc gccggcgcgg tccccggtgg caccagaggt
    11641 ggcgtccccg gtggaggacc cggtttccga ggaaccgagt tcgacacggc gcggcttgct
    11701 cgcttcactg accgggatgg tcagggtcag caccccgttg gccgtgtcag ccgtcatgcc
    11761 gtccggatcg aggttgtccc ccaggaacag ctggcgggca aactcgcagt agggccgctc
    11821 gtcgatgatc acctcatcgc cctcccgccg tgcgggggcg cgacgggcgc gcacgctcac
    11881 cacgttgcgc tcgacggtca ggtcgatgtc ctcggggtgg acgccgggca cgtcgaggta
    11941 gatgagaaac tggttgccgc gccgcagcgc ctccatgggc atggtccgca gggcacgagc
    12001 cccgacggag agcgtctgtt cggccagtcg gtcaagttca cggaacggat cgaatcgcaa
    12061 aagagtcacg gggttcctcc tgcaggacgg gtgcaccgtg ctcacccagc gttgcagcgc
    12121 caccgacgcg gcgattcggt ccgcgggatg caacggcacc tgatctgttc gccccgacag
    12181 gtccatgggc ccacgcgacc tgcaccgacc cgcgtcgccg ggacgttgcg cgcggtccgc
    12241 cgcggcgcac ttccggcgga cgacgccgcc gcgcagcccg cgagcaccga ttcagtgtcc
    12301 ccgcgagcgg gaacacgttc cggggatgag ttcaccggcg tagggtcaac acaaatttgc
    12361 cgcctcggcg ctcgatcgaa cgtttcgacg aggaatggcg aggagtggat gtacaacggt
    12421 cggccgatca ccgcgatgcg cgagcagctc tcgaacctgt gcagcctgtt cacgttgtcg
    12481 atggtgatgt ccgatggacg cgacgagtgg cagatcctcg agctcgccgt cacctcggtc
    12541 ggctcgctca cctcgtgccg ggccgtagcc ggcttcctga ccggcgaggg cggcgaactg
    12601 agctcggaca gcagcaccct cgcgtacccg gagctcacca agcggctggt ggagctcgac
    12661 ggtgccgacg gtcggatcac cgggaccggt gcggcctggg cgtgggccta cgccctgcca
    12721 accggccacg tccatgccgg gtactacgtc gtcgctgccg acgccgagcc accgcagagt
    12781 cagctcctcc tgctgcggat gctcgcgcag cagaccggcc gtgccctcgc caccgcctcc
    12841 ctgcaccggc gggagcggga ggccgccggc cagttgcgcg tgctcaacgc acaactcgcc
    12901 gacgtcaacg accgactcgc cgcaagcgtc tccgacctgg aacggcgccg gagggtccac
    12961 gagacgctca gcgcggtggc agcctccggc gccggcgaag cgggcatctc ccatgcgctg
    13021 caccgcctca cggggttggg agtcgcggtc gaggacccct tcggcaacct gcgcgcctgg
    13081 gccggcccgg gcagacccga tccctacccg cgactcccgg cccgccgccg catcgaactc
    13141 ctggccgatg cccgccgcag tccacggccg gtccgcgacc gcgaccgcat catcgcggtc
    13201 gcccaaccgc gcgacgaggt gctcggcgta ctcgccctgg tcgatcccgg acatcaggct
    13261 ggtgagcacg agttgttcgc gctcgagcac gccaccgtgg tgctgagcat ggagctggca
    13321 cacctgcgga gcctggccga gaacgagctg aggttgcgtc gggacctgat ggacgacctg
    13381 ctcaccggca ccgacgacga gagcgcgctg tcccggtccc aggccctggg tcacgacctc
    13441 cgcacgccgc accgggtcct ggtggctcac tggaccggag cgccgacgga ggacgcagtc
    13501 gtccgtgccg tcgagcaggc ggtgacgcgg gtgctggaca cgagcgtctt gttggcgcgc
    13561 cggtcgggtc gggtggtgct cgtcgcgccg cactccaacg ggcagagcga ccggaaccag
    13621 tggggcgagc tgcaccgcga ggtgacgaag aggctgcgtt cgacgacggg cgcgatcggc
    13681 gtcggcggcg tgtgcgccac accatcggag gtcccgagat cctatgcgga ggctgagcgg
    13741 gcgctgcgag tccggctggg atcgtcggct ccggccggta tgacgatata cgacgatctc
    13801 ggcatctacc ggctgctggt tctcggggag gacgaccacg aggtgaagca gttcgtccgg
    13861 gagtggctcg ggccgctgct cgactacgac gaagcgagca gatccgacct ggtcaccacc
    13921 ctgtggcagt acttcgagtg cggtggcaac tatgacaaca cggcacgggc gctgctcatc
    13981 caccgcagca cgctccgata ccgcctgcgc cgcatccgag acctcagtgg ccatgatctg
    14041 ggcgtggtgg acagcaggct caacctgcac gtcgccaccc gggcctggca gattctccga
    14101 ggtctctcgt gatgcgcggc atcggcggtc gagccactca ctagggattc cgcggaaggc
    14161 attgcaggac ccggtacccg cgcaccgagg tgacacgtgt cgtgggccag ccctgctcca
    14221 ccagccagcg gtggagcgag tcggagccga ggttgcgctg caccacgagg tacgcctcgc
    14281 cgtccggttc gaggcgcccg agccagtggc tgagcatcgc gtgcagcttc ggcttgccga
    14341 tccggatggc cgggttggag cacacgaacc ggaagcggat ctcggccggc acctgctcgg
    14401 gacggcacac ccggacattg ttcagcccca gccgctgcgc gttggcgcgg cacagctcga
    14461 gcgctcggtc gttgacgtcg accgcccaca cggtggcatc gggcgcgaag gacgccatgg
    14521 tgagcgacag cggcccgtag ccgcagccga tgtcgagcag gtcaccgtgg cggccggaga
    14581 agtcgacggc gtcgaggagg accgcggtgc ccgggtcgac ccgcgatccc gagaacactc
    14641 ccggcgcggt tgccagtcgc acctcgcggc cgccgatccg cagggggacc acctgcgcgc
    14701 gttcgccggc ggcgagcccc cgcgcgggag actcgaagta atgcgccatg gtggcatgat
    14761 ggcaccccgc cgcacggccg gccggaagcc tgcagtgcgc cgacgtggat ctactggtcg
    14821 ggtggggtca gcgccggacg ccgccgggag cgggcggccc tgcggcgcat cgccgccttc
    14881 gccagctcct cgtcgacacc gacccagatc tgaccgtcgt cctcccggac ggggaagacc
    14941 gtgatcggcc gcgtcgccgg cgggcagagc ggttcaccac tctcgtagtc gaacacgctg
    15001 ccgtggcacg agcacagcag cccctcggcg gtggccttgc cgctggagag caggcagtcg
    15061 aggtggctgc agtagttcga cgtggcatgc accgtgccgt cgctgcgcga gagcgcgatc
    15121 tcgacgtcgc cgacgaacat ccggcggacc acgccttccg ggatctggcc cgagcgggcg
    15181 acattgtgga actccacggt gttctccggt tctcccgtca ggcgtcgacg gtgtcgacgg
    15241 tgtcgagcag ggcgagcagg tcggtgtcct cgtcggccag cacggtctcc gcaaccggca
    15301 cagcgccggt gatcagctgc cgagcggcgg tgacgtcacc gccggagttc acggcgaaag
    15361 cggcatggac ccggccctcg tgcacgtaga aggcggtgaa ctcgcggtcg ctgatgctgc
    15421 cccgcacgac gaccgtggcg tcggcccgcg ggcgtcccac gaactgcagg ttgtgctcgt
    15481 actggtccga ccagaaccag gcgggatcct cggcgacctc cgcctgcccc agcagtgctc
    15541 tcgccaccgc catcccctgg cggccggcgt tgtcgaggtg ttcggcacgg acgtgccgcc
    15601 cggtggtcgg gtcgaatcgg ctggcgatgt cgcctgcggc gaacacgccc tcgatggcgg
    15661 tgcggcatcc ggcgtcgacg aggacgccgc ccgccacggg atccatcgcc agcccggacg
    15721 cgcgcgcgag ctcgtcgttc ggcaccgcac cgaccgccac cacgagcaga tcggcctcga
    15781 cgacgtcgcc gagccgggta cgcacgacga caccggacga ggtctcgacc accgactcga
    15841 tctgctggcc gaagcggaga tcgacaccgt tctcgcggtg caggtcggca tagacacagg
    15901 ccatcgccgg gccgagctgc ccgagcagcg gcagggcggc tgcctccaga caggtcacct
    15961 cgacgccgag gtcgcgcccc gacgcggcta cctccgagcc gatcagcccc ccgccgatga
    16021 tcgcgatccg cccggcgcca gggagctgcg ccctgagccg gtcggcgtcg tcgagggtgc
    16081 gcaggtggac gacgcggttg ccctcgatac cgggcagcct tcgtgcgcgc accccggtgg
    16141 cgaccagcac cgcgtcggcg acgatctccc caccaccggc gagatgcacc cggcgtgcgg
    16201 acgtgtcgta ggagatccgc tcggcccgtg tcccgagcag cagctcgacg tcgttggcgc
    16261 tgcaccacga cggctggagg atggtcaggt cgtcgaggtc tgcctcaccg cggaggtact
    16321 ccttcgacag cggcggccgc tggtacgggg catgccgctc gtcgccgagc acgaccagcc
    16381 tgccctcgaa cccgcggcgg cgcagcatcc gtaccgccga cacggcggcc tggcctgcgc
    16441 ccaccgcgac aacggtggag ggggtagcgg acacggacgc tcctggggtg tcgggagaac
    16501 gggcggctag gagggttcag cgcgctccta gatctggcgc gggcggggat cgacgtggac
    16561 caccccgtcc accacgagca cgtcgtgcac gggctggcag tcgtcctggt cgtcggcccg
    16621 gccggtctcg gggtcgaagg tccactggtg ggcgggacag acgacctttc ccttccacag
    16681 gctgcctcgg gagagcgagc ggcccttgtg gacacaggtg tcgtcgagcg cgaacacgcg
    16741 gtctccgacg aggaacagcg cgatggcccg gccggcgacc tcgacctgct tcttcctgcg
    16801 acgttgcagt tccaccagcg gagccaccgc gacccactcc acggtccctg cttccggggt
    16861 catggtcctc ctccgttcaa cgggtgcggc ggtgttcgac aggtgcgacg gtcccggtgg
    16921 gtgcgctgga cgggcgcacc caccgggcag gtggatcagc cggcggcgcc ggccggggtc
    16981 gtggcgacgt aggtgtcgta gagcgccctc gtgtaggaga agcgcatctc cgcgccccac
    17041 cgcacgaagt cgaggcagcg ctgctgcagc tcgacggtgt cggcgtggtc gagcacgatc
    17101 tggtagcccc gctcgccgtg gacctcgtcg gaggtgatgt ggaggtcgaa gaactcgatc
    17161 tcgtcctcgg tgaagccgta ggcctcccgc agcggcacga tctgcttctt gtagatgccc
    17221 ggcacctggg actcgagccc cactacgagg gcggccgtgg ccacgacgaa gtgctcgcgc
    17281 atcgccaccg cgtagcacca ggactgcagc cccctggtca ccgcattcat gttgttcggg
    17341 tcctcgatgc gctccttggt ggtgccgcag gcctcgccga accggatgag caggtcggtg
    17401 tggcggatgt cggccagctc ctcctcgtac atgttctgga gcgtgaagtc cttggcgtcg
    17461 gtgaagcggt cgggggtgtt gccgtagatg tagccgaggt agtcggcgaa ggggccgacg
    17521 tagtggtagt ggttctcggc ccaccgcgcg aagtgctcgc gggtcaggag cccatctgcc
    17581 catgcctggc tgaacgaggc gttcttggct tcgcggccct tgatcgcgtt ctcgagcgca
    17641 gcacggaact caccacgtga gagcagctgc ggtgcgtcgg atgtggtggc ggagctgtcg
    17701 accgtcagag tcatcagggc ctctcctgga gagtgtggaa ccgagctgat gccctgaacg
    17761 gtaaggacga aggacaccgc tgggctcggt catcgtgcac aacaaccgcc aaaatgttgc
    17821 agcaccctgc accggctggc gcagggtgct gcggtggaga accagcaggt cagaacatgg
    17881 ttgcggccac aagggtggct acgacgagcc ctgcgacgac gggcacgaag catttgcgag
    17941 cgaggtcaat gaccggaacg cgcgtgaccc ccgcaaccac catgagggac gaccaggcga
    18001 ccaggactcc gccgcccgcc cagaccgagc ccatctgccc gacggcggca agcgtcgccg
    18061 gatccatgcc gacggtgtga ccgagcgcac cggagagcga tcccgtcaac gggagcccgg
    18121 agaagcccga gccctccagc cccgtggcca tgccgaccag cagcaccccg aagcacatga
    18181 ccagcgggtt cccgggaatg aaccgctcgc ctgccgtgac caggtcgacg agcaggcccg
    18241 gcgctttctg gccgtcgggg aggccgatga tctttgccgc gtagtcggcg ttgccgagga
    18301 agaagaaccc cgcgatcggg agcaccacac ccatcgcctt gaacgcgaag accaagccgt
    18361 cgacgaggtg ctcgccgcag gtcgacagcg ctttggtcgg accgtcgagc gcgaccacgg
    18421 ccacgatcac cagcagggcg gcgacgccac cgaccagccc ggccgcgctc cctcccgcga
    18481 gcggcgggac cagcgtggtg aacttgccca gcgccatgta gacgaccatg acggcgaaga
    18541 tgaccggcac gccgatggcg aagatcctcg acaggcgggc agtccgggcg tcctgctcgt
    18601 cgtcggcggc tgcggccgcg ggctcgggcg aatcgccacc aggtccgtcg ccggcatcgc
    18661 caccgccccc tgccgccggg agcacatcga cgggaggctc acccccgcct cccccgcgcg
    18721 tcgctacgcc cgcaccgacg ggaacccggt cctcgacgac cgtgagcggc tcggcgatta
    18781 tctcgacgct cgggcgtgac ttcgcgattc ccaacttctc cgagat
//
LOCUS       DASRSC010000175         6025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1207352,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000175 DASRSC010000000
VERSION     DASRSC010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207352"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..488
                     /locus_tag="VFE39_08825"
     CDS             9..488
                     /locus_tag="VFE39_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3093 domain-containing protein"
                     /protein_id="HET6258741.1"
                     /translation="MSEHPSAAAPATSGRPRFDERLSVPVWWYLPAIGVAVLLGAEVH
                     MGYPGIRSWIGYVVTVPLFVIALFCLGRMRVRVAAGELHVGAARLPLRYIGRVEIVPK
                     DGKQVALGPELDPAAFLMLRSWVGPVVRLEVTDPDDPTPYWIFSVRDSEALVAALER"
     gene            complement(663..965)
                     /locus_tag="VFE39_08830"
     CDS             complement(663..965)
                     /locus_tag="VFE39_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 domain-containing protein"
                     /protein_id="HET6258742.1"
                     /translation="MATDYDAPRRNETDDLAEDSLDELKARRTEAQSSVVDVDESDTA
                     ESFELPGADLSGEELTVKVLPKQADEFTCASCFLVHHRSRLAETNGSQYICRDCAA"
     gene            1199..2314
                     /locus_tag="VFE39_08835"
     CDS             1199..2314
                     /locus_tag="VFE39_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET6258743.1"
                     /translation="MALSSDPDVTDDSRGVPDTHPGADATAVGAWLAERLDDTAWRHS
                     TLRPIGAGRSNLTYRVDSPAGAVVLRRPPVGEVAATAHDMGREQRVISALAETSVPVP
                     RVLATSAGGPPVDAPCFVMELVDGVVPLGELPPGWATTEGDRKRVSHALVDVLVALHG
                     IDPDAVGLGDFGRPEGFLARQVRRWVTQWEKARETGIPVSEETLTELTRLADALGAAV
                     PTTQRHTIVHGDYRIDNCLFDAEDPGVIRAVLDWELSTLGDPLADLGLLLVYWRDADE
                     HPAWRAAQYIPSPTQLPGFLGRTELVKAYAAGSGLDLAPLPWYVAFGAFKLAVVLAGI
                     LARVRAGMVPASMAAGLEGSVDPLVALGHHVLAEGLA"
     gene            2314..2991
                     /gene="cei"
                     /locus_tag="VFE39_08840"
     CDS             2314..2991
                     /gene="cei"
                     /locus_tag="VFE39_08840"
                     /inference="COORDINATES: protein motif:HMM:NF035953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope integrity protein Cei"
                     /protein_id="HET6258744.1"
                     /translation="MAGETIRRARPYQRRRRGPVLIVVSVLAVLAVFTWTTVLINAGG
                     AAGTQLCPPPVAGPPPGEPLPSGALNAVTPVPPSSVQVRVLNAGGQRGQANLVAAQLG
                     DLGFSEAAPPDNDPFYPDAKLECTGQLRFGPAGEAAASTVAIVLPCAELVRDGRAEAT
                     VDVAVGTAFGDLNPSRAAQAVLDQLSAGPSRGTDGSANADPNAPDVVPAPPTVDPSML
                     EEARAGC"
     gene            complement(3008..3895)
                     /locus_tag="VFE39_08845"
     CDS             complement(3008..3895)
                     /locus_tag="VFE39_08845"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET6258745.1"
                     /translation="MNEARANVPTDPDPSSLDDPEALRRIAERLVTEAAEYLHGLPRP
                     WEDDLAADSAQSVSTKSTPTDVVTASDHALETLIRQRVAELRPGDAVVGEEHGGTAGD
                     ARTLWVVDPIDGTVNFLYGMPWYAVSVAAVRDGASVAGAVIEPASGRLWSAAVGGGAT
                     CDGRPLRVSAASDVSLSLLGTGFSYSAERRVRQVRMVSGLLPHVRDVRRMGAAALDLC
                     AVAAGWVDAYLEHGCNWWDWAAGALIAAEAGAVVRTPGPTGSVPPDDGLGADALFAAT
                     PGIADEVSALAREHGAASV"
     gene            4153..5568
                     /locus_tag="VFE39_08850"
     CDS             4153..5568
                     /locus_tag="VFE39_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6258746.1"
                     /translation="MAAADSATRIDSSVDTTTSPAPARARSRTGASAKTATKSPRSRT
                     ATPTKKNGPPAKKAAGDPAVDGEPTDLDGADGELDGEPTDLEEVDVELDAELVADAPE
                     GAEVVVDDDDDEDDDEEVANTGANRRSPSQRERSSTPQKSGDFVWDEEESEALRQARK
                     DAELTASADSVRAYLKQIGKVALLNAEEEVELAKRIEAGLYAAERMRRSIDAGEKVSP
                     QLRRDLRWIVRDGERAKNHLLEANLRLVVSLAKRYTGRGMAFLDLIQEGNLGLIRAVE
                     KFDYTKGYKFSTYATWWIRQAITRAMADQARTIRIPVHMVEVINKLGRIQRELLQDLG
                     REPTPEELAKEMDITPEKVLEIQQYAREPISLDQTIGDEGDSQLGDFIEDSEAVVAVD
                     AVSFTLLQDQLQSVLATLSEREAGVVRLRFGLTDGQPRTLDEIGQVYGVTRERIRQIE
                     SKTMSKLRHPSRSQVLRDYLD"
BASE COUNT          884 a         2214 c         2090 g          837 t
ORIGIN      
        1 cggtccccgt gagcgagcat ccatcggcgg ccgcgcctgc gacgtccggc cgtccccggt
       61 tcgatgagcg gttgtccgtg ccggtgtggt ggtacctgcc ggccatcggg gtcgcggtgc
      121 tgctgggcgc cgaggtgcac atgggctacc cgggcatccg gtcgtggatc ggctacgtcg
      181 tcaccgtgcc gctgttcgtg atcgccctgt tctgcctcgg gcgcatgcgc gtccgggtgg
      241 cggccggcga gctccacgtc ggggcggcgc ggcttccgct ccgctacatc ggccgggtgg
      301 agatcgtccc gaaggacggc aagcaggtgg cactgggccc ggagctggat ccggccgcgt
      361 tcctgatgct ccgctcatgg gtcggtccgg tcgtgcgcct cgaggtcacc gacccggacg
      421 acccgacgcc ctactggatc ttcagcgtcc gcgattcgga agcgctggtg gctgcgctcg
      481 agcggtgacc ggcacggcgc cggcccgacc cgacagcccg agaacggccc cggatgcgat
      541 tcgaccccgg accggaggac cggttcgggg cgatctcgct tctacgccct cgagcgccgg
      601 ccggtgggcc gcgctccgga cgtcaggctg ctgatgtcac agcgtgacgt caggggcgcc
      661 cgtcacgcag cacagtcacg gcagatgtac tgcgagccgt tggtctcggc cagccggctg
      721 cggtggtgca ccaggaaaca gctggcacac gtgaactcat cggcctgctt cggcagcacc
      781 ttgaccgtga gttcctcgcc ggacaggtca gcaccaggca gctcgaagct ttccgctgtg
      841 tccgactcgt ccacatcgac gacggacgac tgcgcctcag tccgccgggc cttcaactcg
      901 tcgagcgagt cctcagccag gtcgtcggtc tcgttgcgcc ggggcgcgtc gtagtcggtc
      961 gccatcgttc ccaccctctt aacacacctg tcgtcatcgt gcggctggtc aacgcggcag
     1021 caccggggtt tgtgcccagc atccgagtga cacgcgtctc agccctaccc cggttgcggc
     1081 tcgatcggat atacgccgca actgctctga ctgcccccgg ggcgcagagg gtagctcagg
     1141 ctctcgacac ccgcccggcg ccccgccgtc ctggtgcgcg gccgttaggg tgcccgacgt
     1201 ggctctctcc tccgaccccg acgtcaccga cgattcgcgg ggcgttcccg acacgcaccc
     1261 gggggccgat gcgaccgccg tcggtgcctg gttggccgag cgcctcgacg acacggcgtg
     1321 gcgtcacagc acgctgcgac cgatcggcgc gggccgatcg aacctcacct accgcgtcga
     1381 cagccccgcc ggggccgtcg tgctgcgccg accaccggtc ggcgaggtcg cggccaccgc
     1441 ccacgacatg ggccgggaac aacgcgtgat ctccgcactg gcggaaacga gcgtgcccgt
     1501 cccccgggtg ctggcgacgt cggcgggcgg gccgccggtg gacgcgccgt gcttcgtgat
     1561 ggagctcgtg gacggggtcg tcccgctcgg cgagctcccg ccgggctggg ccaccaccga
     1621 gggcgaccgc aagcgcgtga gccacgccct ggtcgacgtg ctggtcgcgc tccacggcat
     1681 cgacccggac gcggtggggc tgggcgactt cggcaggccg gagggtttcc tcgcacgtca
     1741 ggtgcggcgc tgggtcacac agtgggagaa ggcccgcgag accgggatcc cggtgagcga
     1801 ggagacgctc accgagctca cccggctcgc cgacgcactg ggcgccgcgg tgccgaccac
     1861 acaacgccac acgatcgtgc acggcgacta ccgcatcgac aactgcctgt tcgatgccga
     1921 ggaccccggg gtcatccggg ccgtcctcga ctgggaactg tcgacattgg gcgacccgct
     1981 cgccgacctc gggctgcttc tcgtctactg gcgcgacgca gacgagcacc cggcctggcg
     2041 agctgcccag tacataccga gccccacgca gctccctggg ttcctcgggc gcacggagct
     2101 cgtgaaggcc tacgccgccg ggtcgggtct cgatctggcg cccctgccgt ggtacgtcgc
     2161 gttcggggcg ttcaagctgg cggtggtgct ggccggcatc ctcgcgcggg tccgcgccgg
     2221 gatggtgccg gcgtcgatgg cggcggggct cgagggcagc gtcgacccgt tggtggcgct
     2281 ggggcaccac gtgctcgccg agggcctcgc gtgatggcgg gcgagacgat acggcgcgcc
     2341 cggccctatc aacggcgcag gcgcgggccc gtgctgatcg tggtgtcggt gctggcggtg
     2401 ctcgcggtgt tcacctggac gacggtgctg atcaacgccg gcggtgccgc ggggacgcag
     2461 ctgtgtcccc cgccggtcgc cgggcccccg cccggtgagc cactgccgtc cggtgcgctc
     2521 aacgcagtga ctcccgtgcc accgtcgtcc gtccaggtgc gggtgttgaa cgcgggaggg
     2581 cagcgcggac aggcgaacct ggtggccgca cagctgggtg acctcggctt cagcgaggcg
     2641 gcacccccgg acaacgaccc cttctacccg gacgcgaagc tggagtgcac cggccagctg
     2701 cgcttcgggc cggcaggtga ggccgcggcg agcactgtgg cgatcgtcct gccgtgcgcc
     2761 gagctcgtgc gggacgggcg tgccgaagcc accgtcgacg tggcggtcgg caccgcgttc
     2821 ggcgacctca acccgtcccg ggccgcgcag gccgtgctcg accagctgtc ggcgggcccc
     2881 agcaggggca cggacggctc cgcgaacgcc gacccgaatg cgcccgacgt ggtcccggcg
     2941 ccgccgacgg tcgacccctc gatgctcgaa gaggcccgcg ccggctgttg acctccgcgc
     3001 gcccggtcta cacggacgcc gcgccgtgct cccgcgccag cgcggacacc tcgtcggcga
     3061 tgccgggcgt cgcggcgaag agggcgtccg ctccgagacc gtcatcgggc gggacgctgc
     3121 cggtcgggcc gggcgtgcgc accacggcgc cggcctcggc ggcgatgagt gcgcccgccg
     3181 cccagtccca ccagttgcac ccgtgctcca ggtaggcatc gacccaaccc gccgccaccg
     3241 cgcagagatc gagggctgca gcgcccatgc gccgcacatc gcgtacgtgc ggcagcaggc
     3301 cgctgaccat gcgcacctgc cgcaccctcc gctccgcgct gtaggagaac ccggtgccga
     3361 gcagggacag cgacacgtcg gacgcggcgg acacgcgcag cggcctgccg tcacaggtgg
     3421 cccctccgcc gacagcggca ctccacagcc gccccgacgc cggctcgatc acggccccgg
     3481 cgacggatgc cccgtcacgc acggccgcca ccgacaccgc gtaccagggc atgccgtaga
     3541 ggaagttgac ggttccgtcg atcgggtcga cgacccacag cgtccgggcg tcccccgcgg
     3601 ttcccccgtg ctcctccccc accacggcgt cgcccggccg cagctcggcg acccgctgcc
     3661 ggatcagggt ctccagggcg tggtcggacg ccgtcaccac atcggtgggc gtgctcttcg
     3721 tcgacacgct ctgcgccgag tcggctgcca ggtcgtcctc ccatggtcgg ggtagcccgt
     3781 gcaggtactc ggcggcctcc gtgaccaggc gctcggcgat ccggcgcagg gcttccgggt
     3841 cgtcaaggga cgacgggtcg gggtcggtcg ggacgttcgc acgggcctcg ttcacggaca
     3901 tcatccgacc acacgacacc tgcgggatga cggtcctgta cccgctcagc cacgcaccgt
     3961 cgactcttcc cgtggcgagg cgcggacgtc tggagcacac gcagttacaa tgatgcggta
     4021 ccccgatgcg ggatggcctc cagagaacac caggacggcg atccggcgcg gtatccgaca
     4081 acatctcgac ctgcagccgg atctcatacg cgtcccggcg caccgcagag cgcaccgtag
     4141 cgagagggcc cagtggcagc cgcagactcc gcaacccgca tcgactcgtc cgtcgacacg
     4201 acaacgagtc ccgcacccgc acgtgcccgt tcacggacgg gcgcgtccgc caagaccgcg
     4261 acaaagtcgc cgcggtctcg cacggccacg ccgacgaaga agaacgggcc gccggctaag
     4321 aaggccgccg gcgaccccgc ggtcgacggc gagcccaccg acctcgacgg cgccgacggc
     4381 gagctcgacg gcgagcccac cgacctcgag gaggtcgacg tcgaactcga cgccgaactc
     4441 gtcgccgacg cgcccgaggg cgccgaggtg gtggtggacg acgacgatga cgaggacgac
     4501 gacgaagagg tcgccaacac cggcgccaac cggcgcagcc cgtcccaacg ggagcgttcc
     4561 tccaccccgc agaagtccgg cgacttcgtc tgggacgagg aggagtccga ggccctccgg
     4621 caggcccgca aggacgccga gctcaccgcc tccgccgact cggtccgcgc ctacctgaag
     4681 cagatcggca aggtcgcgct gctcaacgcg gaggaggagg tcgagctcgc gaagcgcatc
     4741 gaggccggcc tctacgccgc cgagcggatg cgccgctcga tcgacgcggg tgagaaggtt
     4801 tccccgcagc tccgccgtga cctgcgctgg atcgtccgcg acggcgagcg cgccaagaac
     4861 cacctgctgg aggccaacct ccgcctcgtg gtgtcgctgg ccaagcgcta caccggtcgc
     4921 ggcatggcgt tcctcgacct catccaggag ggcaacctcg gcctgattcg cgcggtcgag
     4981 aagttcgact acaccaaggg ctacaagttc tcgacctacg ccacgtggtg gatccgccag
     5041 gccatcaccc gcgcgatggc cgaccaggcc cgcaccatcc gcatcccggt gcacatggtc
     5101 gaggtcatca acaagctcgg ccgcatccag cgcgagctgc tccaggacct gggccgtgag
     5161 cccacgccgg aggagctggc caaggagatg gacatcaccc cggagaaggt actggagatc
     5221 cagcagtacg cccgggagcc catctcgctc gaccagacca tcggcgacga gggcgacagc
     5281 cagctcggtg acttcatcga ggactccgag gcggtcgtcg cggtcgacgc ggtgagcttc
     5341 acgctgctgc aggaccagct ccagtcggtt ctcgccacgc tgtccgagcg cgaggcgggc
     5401 gtcgtccggc tgcggttcgg cctcaccgac ggccagccgc gcacactcga cgagatcggt
     5461 caggtctacg gggtcacccg ggagcggatc cggcagatcg agtcgaagac gatgtcgaag
     5521 ctgcgccacc cgtcgcggtc gcaggtgctg cgcgactacc tggactgaca acgatcacaa
     5581 gaggcgctct gagccgcgag aacgtggctc ggagggcttt cttgtgtcgc acagctcttg
     5641 tgtcgcacag cccctgccag cggagtcccc tggtcgccat ggctatccgc cacctggtcc
     5701 agggtcaccc ccgaagcaag agcgccatga tggtgccgga tcccggcgga gaccgacctc
     5761 ctcacccacg agtcacccgc tacctgatct gcctgcagga agactggcga ccgcctggtg
     5821 tgcggatcgg ccccgagcga cctccgcaca gcgcccgccg gcgaggaagc tccaaccggc
     5881 cgatctgttc gctgtgcaca gtgcgaccgt ccgggcgccg ttcagcggtg ccggcgccgt
     5941 cgtgcgcggg ctactgccca ctccgagtag tcgctcggcc gcgtggacgg caacctcccg
     6001 gcggtgctga ccacaggtct cgtcg
//
LOCUS       DASRSC010000176        16273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2190516,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000176 DASRSC010000000
VERSION     DASRSC010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 16273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16273
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2190516"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(494..628)
                     /locus_tag="VFE39_08855"
                     /pseudo
     CDS             complement(494..628)
                     /locus_tag="VFE39_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662050.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
     gene            861..1412
                     /locus_tag="VFE39_08860"
     CDS             861..1412
                     /locus_tag="VFE39_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HET6258747.1"
                     /translation="MRHGHGGRGRVFFGHKPGSPFIVSDPLLCIVEIPKGSRNKYEWE
                     PELGLMLLDRFLSASVVFPTDYGFIRGAYSSDGDPMDVLIAVSEPTFPGCGVYARPVA
                     VLWLCDQEEREPKVVCVPCKDPNWQGVSDLGDLPGQLVEEIAHFFVAYKQREGHDVTV
                     DGWGGADEARRAVQDGLARAAHT"
     gene            1469..3406
                     /locus_tag="VFE39_08865"
     CDS             1469..3406
                     /locus_tag="VFE39_08865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HET6258748.1"
                     /translation="MDRPRLAALDGVRALAVVAVLLFHGGVGWLGGGFLGVDVFFVLS
                     GYLITALLLAEWRRTGRLRLGAFWARRARRLLPALVVVVAVVAAVSQFLLPRDEMALL
                     RGDALGALGYVANWRLILRGTDYFAQTASPSPLQHTWSLGIEEQFYLLWPLVVLALLR
                     WTPTRHRRVALLGVVSLGAITSVAVGWLLSGQDADLDRAYFGSDSRAVSILLGCGLAV
                     ALSEHPVVSARARRVCGVAALAGALVIGWMCVHADGADPRFYRGGLLVTALAAVAVLA
                     DAVLVPDSLTARLLSVPPLPVLGRISYGVYLWHWPLFTWLDAERTGLTGSGLLAVRCA
                     VTLLVAGASFVLIERPILAGRRWQLQPRRTLAAAGTALVVTGCSVLVPLATGPPPVSQ
                     LVDNPRDNDTSALPALPDGPSTPARPHHHTPGLPVRVDTFGDSMARTLMKNLPSRPDL
                     IVDDRTMLGCGIALGGPFRYFGSVQQQPAGCAQWPTLIEQATAADDPDVALILVGRWE
                     TMDRVHDGRWTHIGDPSFDDYLRGQLERAVNLATARGAVAALCTQPYNKRGERPDGGL
                     WPEDEPERVNRWNLLVRQVAAAHPHDVHLVDLAARVSPAGHFTYAVDGVQVRSDGVHF
                     TAAGVRWLAPWLVPQLVTAAR"
     gene            3551..4081
                     /locus_tag="VFE39_08870"
     CDS             3551..4081
                     /locus_tag="VFE39_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015102687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258749.1"
                     /translation="MLKRALWQGLVSGAAGAVVMTLGEKVEQRLTGRPDSHMPARVLE
                     RLAGAAEQPRRQPLPVNWAMHFGQGALIGVLRSVMANVGLRGPAASAMFTVVRLTNDQ
                     ILENATGVGAPPPTWPRPELVVDLVHKAVYAFTTGVVADALAASSGPGPGQRHAALRP
                     GRESDVGPLPRAAASA"
     gene            4535..6043
                     /locus_tag="VFE39_08875"
     CDS             4535..6043
                     /locus_tag="VFE39_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6258750.1"
                     /translation="MTTYAPEYLDLVTSRDSGRVTEAFRREIAGLVSEGGLISEPAQL
                     RTYECDGLTGFRVVPALVVLPATTDEVAAVVRLCARERVPFVARGAGTGLSGGSLPVA
                     DGVVIGLARMRRVLSVDVANQRAVVQPGVTNASISAMVAQHDLYFAPDPSSQVVCSIG
                     GNVAENSGGAHCLKYGFTTNHVYALTFVTADGSVVTLGGDSLDPVGPDLRAVVLGSEG
                     TLGIVTEITVRLLRKPEAVRTLVADFPDAEHAGNAVSDIIAAGIVPAAVEMLDTLAIQ
                     ACEKAVQAGYSLGCAAALVVELDGVDAEVEEEFVRVQEICRDNQTTNIRIANDPDERA
                     KVWKGRKAAFAAMGRISPDYFVQDGVIPRTKLGSTLSRIREMSTEAGLRVANVFHAGD
                     GNLHPLVLYNAAVPGEAERAEHLATAIVELCVEQGGSITGEHGVGSDKACSMLKMFSP
                     EDLTVMARIRAAFDPDGICNPGKVLPTPRLCGERPGPYRPHPLEASGVIERL"
     gene            6060..7280
                     /locus_tag="VFE39_08880"
     CDS             6060..7280
                     /locus_tag="VFE39_08880"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6258751.1"
                     /translation="MDNSTSLGVERPASVAEAAELLHGTRGPLLFRGAGTKQAWGGRI
                     TEPALVVETAELSRLLAYNPADMTVAVEAGMALRDLQEILGEKGQWLALDPPSAATGA
                     TVGGLLATGDSGPRRLRYGALRDLVIGVTLVLSDGTVAHGGGTVIKNVAGYDLPKLLY
                     GSLGTLGLVAEVVLRLHPRPETSATVTVASSVDRAADVALRLLASPLEPSAVDWVQTG
                     GGLLAVRLEGTRAGVEAQRRAVVEMLTEDGARADWHTGADEDALWQELTAPHRPAPDA
                     TLAYVGTLPSRTTDVARTLAEGGEAGGATTELASRPALGLHTARFTGAPDGVAKAFEA
                     WRAQLLGMGARAVLHERPADVDVLVDALGPAPSSAGLLQALQHRFDPDRRCGPGRLGP
                     WLAPSPTREDTPQP"
     gene            7277..8698
                     /locus_tag="VFE39_08885"
     CDS             7277..8698
                     /locus_tag="VFE39_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET6258752.1"
                     /translation="MTQPTRHDENQRVTVEPPAAVGNGAGVFDLHHPPSDDLISDCVH
                     CGFCLPTCPTYLLWGEEMDSPRGRIYLMKIGREGSVPLDDTYTQHFDACLGCMACVTA
                     CPSGVQYDKLIEAVRPQLERNHSRRWDDRAFRGMIFSLFPYPSRLRVAAVFGAAYERI
                     GGHRLLERSGLLKFLPKRLQAVEALMPPAHLRDLAKRTPAFTPAVGTARRRVGFLTGC
                     VQRVFFADVNAATVRVLSAEGCDVVAPRKQRCCGALSEHAGREPEALDRARRLIDAFE
                     DTGVDTIVSNVAGCGSSLKEYARLLRDDPEYAERAAAFSAKARDVSELLAELGPVAPR
                     HPIEATVAYHDACHLGHAQGVRAQPRAVLGTIPGLQMTDIPEAEICCGSAGIYNLVNP
                     EPAEELGRRKVDNVLSVSPDALATSNPGCLLQIRRYWPGDLPMFHPVELLDASIRGVD
                     PIAAAGRRSQDKLEARTAEPGTF"
     gene            8763..9587
                     /locus_tag="VFE39_08890"
     CDS             8763..9587
                     /locus_tag="VFE39_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005456685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET6258753.1"
                     /translation="MTDHPLRYDINLSILFTELPLLERPAAAAAAGFTAVEFWWPFDA
                     AVPDGSDVDRFAAAVEDAGVQLVGLNFYAGDMPGGDRGLVSWPARSAEFRANIGVALA
                     LGARLGCTAFNALYGNRVDDATPEQQDELAAENLALAAKAAAEQGAVVLLEPVSGAPR
                     YPLRTAADVVAVMDGVERDAGADNLRLLFDLYHLTVNGDDIPAAIDAYASRTGHVQIA
                     DAPGRHEPGTGNIDIDGHLAALADAGYSGWVGLEYKPSGSSTDSLDWLPRARRSSS"
     gene            9619..10533
                     /locus_tag="VFE39_08895"
     CDS             9619..10533
                     /locus_tag="VFE39_08895"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HET6258754.1"
                     /translation="MTTVAFIGLGIMGGPMAGHLVDAGFDVVGFNRTPDKVAALVERG
                     GRGAGSIAEAVRDADIVAVMVPDSPDVQDVLAGEDGVFDTARKGTLVIDFSSIRPDVT
                     AALAEQAKAAGLRLLDAPVSGGEAGAKNASLSIMVGGDPADYEAAQEVLQAVGTTIVH
                     VGPSGSGQTVKAANQLIVAGNIQLLAEAITFLEAYGVDMAAALKVLGGGLAGSKVLDQ
                     KGEGMLARSFEPGFRIELHHKDMGIVTSAAREAGVVIPLGAVVAQLMASARANGEGGL
                     DHSALLRGVLRLSGRDPDRTDTDDSDTA"
     gene            10547..12322
                     /gene="gcl"
                     /locus_tag="VFE39_08900"
     CDS             10547..12322
                     /gene="gcl"
                     /locus_tag="VFE39_08900"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HET6258755.1"
                     /translation="MPRMRSVDAAVAILEKEGVDTVFGLPGAAINPFYSAMRDNGTIR
                     HYLARHVEGAAHMAEGYTRAAAGNIGVCVGTSGPAPTDMITGLYSAQGDSIPILAITG
                     QAPVARLHKEDFQAVDIAAIAAPVTKMAMTVLEPAQVPGAFQQAFHLMRSGRPGPVLL
                     DLPFDVQMAEIEFDPDTYEPLPVYKPRATRAQAVKALDMLQRSERPVIIAGGGVVNAN
                     ASHLLVELAEVLGVPVIPTLMGWGSIPDNHRLMAGMAGLQTAHPYGNATLLASDFVFG
                     IGNRWANRHTGGLDVYRSGRTFVHIDVEPTQIGRVFPPDYGIVSDAGAALEVLLEVAK
                     ERAAAGRLPDRSEWADECFERRRTMVRRTHFDDIPVKPQRVYEEMNKAFGPETRYVST
                     IGLSQIAGAQFLHVFHPRHWINAGQAGPLGWTIPAALGVCVADPQATVVALTGDYDFQ
                     FMMEELAVGAQFNLPFVHVLVNNAYLGLIRQSQRAFDMDFCVQLSFDNINAPEVNGYG
                     VDHVKVAEGLGCKALRVVEPAEILPALEDAKKLMAEHRVPVMVEVILERVTNISMGTE
                     LDNVVEFEDLAASHEDAPTFVALLD"
     gene            complement(12362..13576)
                     /locus_tag="VFE39_08905"
     CDS             complement(12362..13576)
                     /locus_tag="VFE39_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET6258756.1"
                     /translation="MTGHVETNEAHRTSPPGRGIFDPQLLDRCISCGFCLPACPTYAV
                     SQDEGSSPRGRITLMRALETGRLDEDDPRLREESSFCLGCRACEPVCPAGVQYGDLLE
                     EWRDHQWRGRHRPPLAQVLMMVAERTPLLRAMGVVRRHARTAPRPADTSLMLGCFERG
                     LYPGLSWTVRTLLPDIAVPAQQGCCGALHAHNGESERGRELARRLGEELPGVIVTTAG
                     GCAAHLAGVLGRDRVHELSEHLAKTGAAPVGEVTVDGRRARVAIQDSCHLRNGLRVFA
                     EPRALISTVAEFVPVTGDDSCCGSAGTYSLLRPRDSRRILDPKLDAFEAAAVDYVVAV
                     NPGCLRQLQTGLRRRGSAIRAVHLADLLAAAAGGRPLPRGVPWRVPRILTRLGRGSRQ
                     RGAARSTDGWSA"
     gene            complement(13573..14955)
                     /locus_tag="VFE39_08910"
     CDS             complement(13573..14955)
                     /locus_tag="VFE39_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6258757.1"
                     /translation="MSLDRALVAELQAIVGGEHVRTDRGDVEPYARDATPVFRAVPDA
                     VVWPRTSEEVAAVLRLATQRHVPVVPRGAGSNLCAATVAERGGIVLVLTRMNRILEIS
                     PDELLARVEAGVTTAALADAAAAKGLLYAPDPGSRTVSTVGGNVATCAGGLRGLKYGV
                     TRNYVLGATAVLPTGEVIRSGGRLWKDVAGYDLTRLLTGSEGTLGVLTEVTVALLPMP
                     ATTNTGVAYFPTLADAGRAVGAIIGDGIVPATLEFLDAKCIGAVEEYANLGLRLDAGA
                     LLLFGDDGPADVVDRNLGRIGEQCTANGAMEVTLAESVARSEALLTARRCSLPALSRL
                     GTLTLLEDATVPRPRLAEMVDRIDDIADRYDVPIATFGHAGDGNLHPTCVVDQRDEAA
                     IDRAHKAFGDVFAAAIDMDGTITGEHGVGAAKLPYLQARLGADQMALLRRIKAAFDPA
                     GILNPGKLGS"
     gene            complement(14952..15611)
                     /locus_tag="VFE39_08915"
     CDS             complement(14952..15611)
                     /locus_tag="VFE39_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HET6258758.1"
                     /translation="MGAREEVLGRIRAALTDVPTDEDIEIPRGYHRSRVQPEGTVERF
                     RERVADYKATVEQVSADGVAAAVDKALRTVGASRVAVPPGLPTEWVPGDGAVTLVQDS
                     RQAPLSVAELDAVDAVVTGSVVGIAETGTIVLESGELCGRRALTLVPDVHVCVVRADD
                     VVDDVPAAVARLRSDRPLTWISGPSATSDIELDRVEGVHGPRTLHVLVVTGDDAGGDR
                     P"
     gene            complement(15611..>16273)
                     /locus_tag="VFE39_08920"
     CDS             complement(15611..>16273)
                     /locus_tag="VFE39_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET6258759.1"
                     /translation="GERMNPYTSMWTGVSEGDGPQSVHIVLVDNGRTRALADPVGRQA
                     LRCIRCSACMNFCPVYERTGGHAYGSVYPGPIGAILNPLLNGVDAGPVEKSLPYASSL
                     CGRCYEVCPVAIDIPDVLVHLRGKVVDASREHPVPNAEMLGMRAAGWTFDRPGRLAAV
                     QRLAGFGSRLLGDRAGLGPIPFLGPAAKWSRARDIPLPPRESFRAWWQRTRGKQSRKG
                     AS"
BASE COUNT         2290 a         5960 c         5698 g         2325 t
ORIGIN      
        1 gcttctacga cacacccggc atcgaccccc tcagcctcgt ccgcgtcgct gccaccgccg
       61 tcaccggcgg cgacccgggt gccgcggggg cggcctcatg gacgcataca cgtgcccgcc
      121 cacgtagcac cgcctggccg tcgtcgacga tggccttgtc ggcggcggac gcggtgacga
      181 gtgcctggag ggcgaggttg tttggcggcc ctcgggtagc cgcggctggt ctggtagatc
      241 agcgcggcgg cgtggccggc acccaggcga agcaccagcg ccgcgaaagg gactcgtgac
      301 acccgaccgt gacgccatcc gctacgagag gtactccggc gagggctgca gccgctccgg
      361 agcacccaac ccgtgaccgc tgccgtcgaa cggctccggc ccctgcggac cgagcgctcc
      421 gcagctccgc agctcggcga tctgatccgc gctgtacccg gacgcggtga ggacacggga
      481 ggtgtcagcc cgaagggccc caacggcgcg tctcgtcacc ggtgggcgcc tcgaccttgg
      541 tcacctccgc gccgtggtcc gcgagcatct gcgcgcagaa gggaccagcg aggacccggc
      601 tcaggtcgag gacccgcacc ccgcagatcg cgcctgcgag cggttcgggg ggccccgcgc
      661 tcacgccggg atccctgggc gacgcaccga gagatcgatg ccgcgcctaa gccggaaggt
      721 tgagtgtcca ggtcaaggtc gggttggtcg tcggcttcga cgtgggagat ccaggtgtcg
      781 aggcgttcgc gtcggctcca ccacgttggc ggtcccccgt tcgggcgcgt cgaccggggg
      841 caccggcgtc gcgtccgcgg atgcggcacg gacacggcgg gcggggtcga gtgttcttcg
      901 gtcataagcc gggtagcccg ttcatcgtgt ccgacccgct tctgtgcatc gtggagatcc
      961 cgaaaggatc gcggaacaag tacgagtggg aacccgagct cggactgatg ctgttggaca
     1021 gattcctgtc cgcttcagtg gtgttcccca ccgactacgg cttcatccgc ggcgcctact
     1081 ccagcgacgg tgacccgatg gacgtgctca tcgcggtctc cgagccgacg ttccccggct
     1141 gcggggtcta tgccaggccg gtcgcggtgc tctggctgtg cgaccaggag gaacgcgagc
     1201 cgaaggtggt ctgcgtgccc tgtaaggacc cgaactggca gggcgtgagc gatctgggcg
     1261 acctgcccgg ccagctggtc gaggagatcg cccatttctt cgtcgcctac aagcagcggg
     1321 agggccacga cgtcaccgtc gacgggtggg gtggcgcgga cgaggcgcgg cgggcggtcc
     1381 aggacggcct cgcccgcgcg gcgcacacct aaatgctcgc cccggcgcgc cgcgctcacc
     1441 agcgcagcag gctgggatga ccccggggat ggacagacct cgcctcgcgg cgttggacgg
     1501 cgtccgcgcc ctggcggtgg tggcggtgct gctgtttcac ggcggggtcg ggtggctcgg
     1561 aggcggtttc ctcggggtcg acgtgttctt cgtactcagc ggttatctga tcaccgcgct
     1621 gctgctggcg gagtggcgac gtacagggcg gctcaggctc ggcgcgttct gggcccggcg
     1681 agcacggcga ctcctgcccg cgctcgtcgt ggtggttgcc gtcgttgcgg cggtgagcca
     1741 gttcctcctg ccccgggatg agatggcgtt gctacgcggt gacgccctcg gcgcactggg
     1801 ctacgtggcg aactggcggc tgatcctgcg gggaaccgat tacttcgccc agacggcctc
     1861 gccgtcgccg ctgcagcaca cgtggtcgct cgggatcgag gagcagttct atctgttgtg
     1921 gccgttggtg gtcctcgcgc tgctgcgatg gacgccgacg cgccaccgac gggtcgcctt
     1981 gctcggcgtt gtcagcctcg gggccatcac atccgtggcc gtcgggtggc tcctctcggg
     2041 gcaggacgcg gacctcgacc gagcgtattt cggtagcgac agcagggcgg tgagcatctt
     2101 gctggggtgc gggctcgcgg tcgcgctgag cgagcacccg gtggtgtccg cgcgtgcgcg
     2161 gcgggtctgc ggtgtggccg cgctggccgg cgctctggtc atcggctgga tgtgcgtgca
     2221 cgccgacggt gcggatccgc ggttctaccg gggaggcctg ctggtcactg cgctcgcggc
     2281 ggtcgccgtg ctggccgatg ccgtccttgt ccctgactcg ctgaccgcgc ggctgctgtc
     2341 ggtgcccccg ctgcccgtcc tcgggcgcat ctcctacggc gtctatctct ggcactggcc
     2401 gctcttcaca tggctggacg ccgaacgcac cgggctgact gggtccggcc tgctcgccgt
     2461 gcgctgcgca gtgacgctcc tggtggccgg cgcttccttc gtgctgatcg agcgaccgat
     2521 cctcgccggg cgacgctggc aactgcagcc acggcgcacg ctcgcggcgg ccggcaccgc
     2581 gctcgtggtc accgggtgca gcgtcctcgt ccccctcgct accggtccgc ccccggtatc
     2641 ccaactcgtc gacaacccgc gcgacaacga cacctcggct ctgcccgccc tgcccgacgg
     2701 cccatccacc cccgcccgtc cccatcacca cacaccgggc ctcccggtcc gggtggacac
     2761 cttcggtgac tccatggcgc ggacgctgat gaagaacctg ccgtcccgac ccgacctcat
     2821 cgtggacgac cgcacgatgc tgggctgcgg catcgccctc ggcgggccgt tccgctactt
     2881 cggatccgtc cagcagcaac ccgccgggtg cgcccagtgg ccgacactga tcgagcaggc
     2941 gaccgccgcc gacgaccccg atgtcgcgct catcctggtc ggccgctggg agacgatgga
     3001 ccgggtacat gacggccggt ggacccacat cggtgacccg agcttcgacg actacctacg
     3061 tggccaactg gagcgtgcgg tgaacctcgc aaccgctcga ggcgccgtgg ccgcattgtg
     3121 cacccagccc tacaacaagc ggggcgagcg ccccgacgga gggctctggc ccgaggacga
     3181 acccgagcgg gtcaaccgct ggaacctcct ggtccgccag gtcgccgcgg cgcacccgca
     3241 cgacgtccat ctggtcgacc tcgccgcgcg agtcagcccc gccggccact tcacctacgc
     3301 ggtcgacggc gtccaggtac gcagcgacgg ggtgcatttc accgcggccg gcgtacgctg
     3361 gctcgccccc tggctcgtac cgcaacttgt caccgcggct cgatgagaca gcagtagcag
     3421 tagcagcacc ggctcggact gctggtgtgc ggtcgtcttc cggccttcgg gtgcatccac
     3481 acgacgcatt tacccgttcg tcgctcgatc aaagggggtt gatcacggtg gcgaggggta
     3541 gccgctgggc atgttgaagc gtgcgttgtg gcagggcctg gtctccgggg ccgctggtgc
     3601 ggtggtgatg accctggggg aaaaggtcga gcagcggctg accggccgac cggattcaca
     3661 catgcccgcg cgcgtgctgg aacggctggc cggggcggcg gagcaaccgc ggcgtcagcc
     3721 gctgccggtg aactgggcaa tgcatttcgg ccagggtgcg ctgatcgggg tgctgcgcag
     3781 cgtcatggcg aacgtgggac tgcggggccc ggcggcttcg gcgatgttca ccgtggtgcg
     3841 tctgaccaac gaccagatct tggagaatgc cacgggcgtc ggggcgccgc ccccgacctg
     3901 gccacggccg gaactggtgg tggacctggt gcacaaggcg gtgtacgcct tcaccaccgg
     3961 cgtggtcgcc gatgcgctcg cggccagctc cggccccggg cccgggcagc gtcacgccgc
     4021 tctgcgtccc ggccgtgagt cggacgttgg gccgcttcct cgcgccgccg cttccgcatg
     4081 acgccctgac gctggccgaa cttccccgga ccgcgttcaa gggcgacctc ctcgcaccgc
     4141 gcctcgggcg cgtggtgcaa cgcctatccg accggcgcac cgcagcactg gtcagccacg
     4201 ccgtagtggc gaatcttcgc cgacagggtg tgcaacgcgc cgacgtcgac gccgctcgcg
     4261 cccgcgcttt cgaccactcc gcacctacgc cgccattcgt tccgccggac gccggaacac
     4321 cgacaaacgg attccatgtg gattccagcg ggaactgccg cgttacgcaa atcgcgttgc
     4381 aggaatgcgt ttcagatgct gtggcggcgc ctggatcacg aagcgacgtt cggccacgac
     4441 cagcgtcggc ccgttggttt gcccggttgc cgtgtgggta cgcccaggct cgcctggccg
     4501 ggttggcgcg cgatcaagcc gaggaggcac ggcaatgacg acgtacgctc ctgagtacct
     4561 ggacctcgtc accagccggg acagcgggcg agtgaccgag gcgtttcgtc gggagatcgc
     4621 cgggctggtc agcgagggcg ggctgatctc cgagcctgcc caactgcgaa cgtacgagtg
     4681 cgacgggctc accggcttcc gcgtggtccc ggcgctcgtc gtgctgccgg cgacgacgga
     4741 cgaggtcgcc gcggtggtac ggctctgcgc ccgcgagcgc gtaccgttcg tggctcgcgg
     4801 cgcgggcacg gggctgtccg gcgggtcact ccccgtggcc gatggcgtcg tgatcggact
     4861 ggcccggatg cgacgcgtcc tgtccgtcga cgtcgccaac cagcgcgcgg tggtgcagcc
     4921 cggcgtcacg aacgccagca tctcggcgat ggtcgcccag cacgacctgt acttcgcccc
     4981 cgacccgtcc agccaggtgg tgtgctccat cggcggcaac gtcgcggaga attccggcgg
     5041 cgcccactgc ctgaagtacg gcttcaccac caaccacgtg tacgcgctga cgttcgtcac
     5101 cgccgacggc agcgtggtca cgcttggcgg cgactcgctg gaccccgtcg ggccggacct
     5161 ccgcgcggtc gtcctcggat ccgagggcac gctgggcatc gtcaccgaga tcaccgtacg
     5221 actgctgcgc aaacccgagg ccgtgcgcac gctcgtcgcc gacttccccg acgccgagca
     5281 cgcggggaac gcggtcagcg acatcatcgc cgccggcatc gtcccggccg ccgtggagat
     5341 gctcgacacg ctggcgatcc aggcctgcga gaaggccgtg caggccggct actcgctggg
     5401 ctgcgccgca gcactcgtcg tcgagctcga cggggtcgac gccgaggtcg aggaggaatt
     5461 cgtccgggtg caggagatct gccgagacaa ccagacgacc aacatcagga ttgcgaacga
     5521 cccggacgag cgggccaagg tctggaaggg ccgcaaggct gccttcgcgg cgatgggacg
     5581 gatcagcccc gactacttcg tccaggacgg cgtcatcccg cgcacaaagc tgggcagcac
     5641 cctgagccgg atccgcgaga tgtcgactga ggcgggactg cgcgtcgcca acgtcttcca
     5701 cgccggcgac ggcaacctgc atccgctcgt cctctacaac gccgccgtgc cgggagaggc
     5761 agagcgggcc gagcacctcg ccacagcgat cgtcgagctg tgcgtcgaac agggcgggtc
     5821 catcaccggc gagcacggcg tcggcagcga caaggcctgc tcgatgctga agatgttcag
     5881 cccggaggac ctcaccgtga tggcccggat acgggccgcg ttcgatcccg acggcatctg
     5941 caaccccggc aaggtcctgc cgaccccccg gctgtgcggt gaacgccccg gcccctatcg
     6001 cccacacccg ctggaggcgt cgggtgtcat cgagcggctg tgaggttccg atgaccgtca
     6061 tggacaacag cacgtcgctg ggtgtcgagc ggccggcctc ggtcgccgag gccgccgagc
     6121 tgctgcacgg cacccgcggg cccctgctct tccgcggcgc cgggacgaag caggcctggg
     6181 gaggacgcat cacggagccc gcgctcgtcg tcgagaccgc cgagctgtct cgcctgctgg
     6241 cctacaaccc ggcggacatg accgtcgccg tcgaggccgg gatggccctg cgcgacctcc
     6301 aggagatcct cggcgagaag ggccagtggc tggctctcga tccgccctcg gcggccaccg
     6361 gcgcgactgt cggaggcctg ctcgccaccg gcgactccgg accgcgacga ctgcgttacg
     6421 gcgccctgcg cgacctggtc atcggcgtca ccctcgtcct gtcggacggc acggtcgccc
     6481 atggcggcgg cactgtgatc aagaacgtcg cgggctacga cctgcccaag ctgctgtacg
     6541 gatcactggg caccctcggc ctcgtcgccg aggtcgtgct gcgcctgcat ccccgaccgg
     6601 agacgagcgc gaccgtcacg gtggcgagct cggtggaccg ggcggccgac gtggccctgc
     6661 ggctgctcgc ctcaccgctg gagccgtcgg ccgtcgactg ggtgcagacc ggcggcgggt
     6721 tgctcgccgt ccgcctcgaa ggcacgcggg caggcgtcga ggcccagcgc agggccgtcg
     6781 tggagatgct cacggaggac ggggcgcggg ccgactggca caccggggcg gacgaggacg
     6841 cgctctggca ggagctgacc gcccctcacc gtccggctcc ggacgcgacg ctcgcgtatg
     6901 tcggcacgtt gcccagccgc acgacggatg tggcccggac cctggccgag ggcggcgagg
     6961 cgggcggtgc gacaacggag ctcgcctccc ggcccgcgct cggcctgcac accgcccgct
     7021 tcaccggagc acccgatggg gtcgcgaagg ccttcgaggc gtggcgggcg cagctgctcg
     7081 gcatgggcgc gagggccgtg ctccatgagc ggcccgcgga cgtcgacgta ctggtcgacg
     7141 ccctggggcc ggcaccgtca tcggcgggtc tgctgcaggc gctgcagcac cgcttcgacc
     7201 ccgaccgccg ctgtggcccg ggccggctcg gcccgtggct cgcgccctcc cccaccagag
     7261 aggacacgcc ccagccatga cccagcccac caggcacgac gagaaccagc gcgtgactgt
     7321 cgagccgccg gcggccgtcg gtaacggtgc cggcgtcttc gacctgcatc acccgccctc
     7381 cgacgacctg atctcggact gtgtgcactg cggtttctgc ctgcccacct gcccgacgta
     7441 cctgctgtgg ggcgaggaga tggactcccc gcgcgggcgc atctacctga tgaagatcgg
     7501 cagagagggc tcggtccccc tcgacgacac ctacacccag cacttcgacg cctgcctcgg
     7561 ctgcatggcc tgcgtcaccg catgcccgtc gggtgtccag tacgacaagc tcatcgaagc
     7621 cgtccgcccg cagctcgaac ggaaccacag ccgccggtgg gacgaccgcg cgtttcgcgg
     7681 gatgatcttc tcgttgttcc cctacccgag ccggctgcgg gtcgccgcgg tcttcggcgc
     7741 ggcctatgag cgcatcggcg ggcaccgact gctcgagcgg agcggtctgc tgaagtttct
     7801 gcccaagcgg ctccaggccg tggaggcgct catgccgccg gcccacctgc gagacctcgc
     7861 caagcgcact ccggctttca ccccggccgt cggcaccgcg cggcggcgcg tgggcttcct
     7921 gaccggttgc gtccagcggg tcttcttcgc cgacgtcaat gccgccaccg tccgcgtgct
     7981 gagcgcggag ggctgcgacg tcgtcgcgcc gcgcaagcaa cgctgctgcg gcgcgctcag
     8041 cgagcacgcc gggcgcgagc ccgaggcact ggaccgggcg cgccggttga tcgacgcctt
     8101 cgaggacacg ggcgtcgaca ccatcgtgtc caacgtcgcc ggttgtggct cgtcgctcaa
     8161 ggagtacgcg cggctgctcc gggacgaccc ggagtacgca gagcgggcgg ccgccttctc
     8221 cgcgaaggcg cgggacgtct ccgagctcct cgccgagctc gggccggtag caccacgtca
     8281 ccccatcgaa gccacggtgg cgtaccacga cgcctgccac ctcgggcacg cccaaggggt
     8341 gcgggcccag ccccgcgcgg tgctcggaac gatccccggc ctgcagatga ccgacatccc
     8401 cgaggccgag atctgctgcg ggtccgccgg catctacaac ctggtcaacc cggagccggc
     8461 ggaggagctc gggcggcgca aggtcgacaa cgtgctgtcc gtcagccccg atgccctcgc
     8521 cacgtccaac ccgggctgcc tgctgcagat tcgccggtac tggcccggtg acctcccgat
     8581 gttccacccg gtggagctgc tcgacgcctc catccgcggc gtcgacccga tcgccgccgc
     8641 cgggcgacgt tcccaggaca agctcgaggc ccgcacagcc gaaccgggca ccttctgacg
     8701 cgacaccccc acccgccgtc cgccgtccct gactcgcagg ggtgccacgg gaaaggatcg
     8761 ctatgaccga tcacccgctg cgctacgaca tcaacctgtc catcctcttc accgagctgc
     8821 cgctgctgga gcgcccggcc gccgccgccg ccgctggctt caccgcggtc gagttctggt
     8881 ggccgttcga cgcggccgta ccggacggga gcgacgtgga ccggttcgcc gcggccgtcg
     8941 aggacgcggg cgtgcagctc gtcgggctca acttctacgc cggggacatg ccggggggcg
     9001 accgtgggct ggtgtcctgg cccgcgcggt ccgcggagtt ccgggccaac atcggcgtcg
     9061 cgctcgccct gggcgcacga ctgggctgca cggccttcaa cgcgctctac ggcaaccgcg
     9121 tcgacgacgc cacccccgag cagcaggatg aactggccgc ggagaacctg gcgctggcgg
     9181 cgaaggccgc cgccgagcag ggcgcggtcg tcctcctcga acccgtcagc ggcgcgccac
     9241 gctatccgct gcgcaccgct gccgacgtcg tcgcggtgat ggacggggtc gaacgggacg
     9301 cgggcgccga caacctccgc ctcctcttcg acctctacca cctcaccgtg aacggtgacg
     9361 acatacccgc cgcgatcgac gcgtacgcat cgcggacggg gcacgtgcag atcgccgacg
     9421 ctccgggacg ccacgagccg ggcacgggaa acatcgacat cgacgggcac ctggcggcgc
     9481 tggccgacgc cgggtactcg ggctgggtgg ggttggagta caagcccagc ggatccagca
     9541 ccgacagcct cgactggctg ccgcgtgccc ggcgcagcag cagctgatga cccgcagcac
     9601 cgatctcgag gagagcccat gacaacggtc gcgttcatcg gtctcggcat catgggcggc
     9661 ccgatggccg gccacctcgt cgatgccggt ttcgacgtcg tcggcttcaa ccgcacgccg
     9721 gacaaggtgg ccgcgctggt cgagcgcggc ggccggggcg cgggcagcat cgccgaggcg
     9781 gtgcgggacg ccgacatcgt cgcggtgatg gtcccggact cccccgacgt ccaggacgtc
     9841 ctcgccggcg aggacggcgt cttcgacacc gcgcgcaagg ggacgctcgt catcgacttc
     9901 tccagcatcc gccccgacgt caccgccgcc ctggccgagc aggcgaaggc cgccggcctg
     9961 cgcctgctcg acgccccggt gtccggaggt gaggccggcg cgaagaatgc gtccctgtcc
    10021 atcatggtcg gcggggaccc ggcggactac gaggctgcgc aggaggtgct gcaggctgtc
    10081 ggcacgacca tcgtgcacgt gggaccgagc ggttccggcc agaccgtcaa ggcggccaac
    10141 cagctcatcg tggccggcaa catccagctc ctcgccgagg ccatcacctt cctcgaggcc
    10201 tacggcgtcg acatggccgc cgccctgaag gtcctcggcg gcggcctcgc cggcagcaag
    10261 gtcctcgacc agaagggcga gggcatgctg gcacggtcct tcgagccggg gttccgcatc
    10321 gagctgcacc acaaggacat gggcatcgtc acctccgcgg cgcgcgaggc cggcgtcgtg
    10381 atcccgctcg gcgcggtcgt cgcgcagctc atggcgtcag cccgcgcgaa cggcgagggc
    10441 ggcctcgacc actccgccct gttgcgcggc gtgctgcggc tctccggccg cgatcccgac
    10501 cggaccgaca ccgacgactc cgacaccgcc tgacaggagc acgcccatgc ctcgtatgcg
    10561 atccgtcgac gccgccgtcg ccatcctcga aaaagagggc gtcgacaccg tgttcggcct
    10621 gcccggcgcg gcgatcaacc cgttctactc ggcgatgcgc gacaacggca ccatccgcca
    10681 ctatctggcc cgtcacgtcg agggtgccgc gcacatggcc gagggctaca cccgcgccgc
    10741 cgcaggcaac atcggcgtct gcgtcggcac ctccggcccc gcccccacgg acatgatcac
    10801 ggggctctac tccgcgcagg gcgactcgat cccgatcctc gccatcaccg gccaggcgcc
    10861 ggtggcccgc ctccacaagg aggacttcca ggccgtcgac atcgccgcga tcgcggcccc
    10921 ggtgaccaag atggcgatga cggtgctcga acctgcgcag gtgcccggcg ccttccagca
    10981 ggccttccac ctgatgcgct cgggacggcc cggacccgtg ctgctcgacc tgccgttcga
    11041 cgtgcagatg gccgagatcg agttcgaccc cgacacctac gagccgcttc ccgtctacaa
    11101 gccccgcgcg acccgggcgc aggccgtcaa ggcactggac atgctccagc ggtccgagcg
    11161 gccggtgatc atcgccggtg gcggcgtggt caacgccaac gcctcgcacc tgctcgtcga
    11221 gctggccgag gtgctggggg tccccgtcat cccgacgctc atgggctggg ggtcgattcc
    11281 cgacaaccac cggctcatgg ccggcatggc tgggctgcag accgcccacc cctacgggaa
    11341 cgcgacgctg ctcgcgtccg acttcgtatt cggcatcggc aaccgctggg ccaaccggca
    11401 caccggcggc ctcgacgtct accggtcggg acgcacgttc gtacacatcg atgtcgagcc
    11461 gacccagatc gggcgcgtct tccccccgga ctacgggatc gtgtccgatg ccggtgccgc
    11521 gctggaggta ctcctcgagg tcgcgaagga gcgcgcggct gccgggcggc tgccggaccg
    11581 ctcggagtgg gcggacgaat gcttcgaacg ccggcgcacc atggtccgcc gcacccattt
    11641 cgacgacatc ccggtcaagc cgcagcgagt gtacgaggag atgaacaagg ccttcggccc
    11701 ggaaacgcgc tacgtctcca cgatcggcct gtcccaaatc gcaggcgcgc agttcctgca
    11761 tgtgttccac cctcgacact ggatcaacgc cgggcaggcc ggtcctctgg gctggacgat
    11821 cccggcagcc ctcggggtgt gcgtcgccga cccgcaggcc accgtcgtcg cactcacggg
    11881 cgactacgac ttccagttca tgatggagga gctcgccgtg ggggcgcagt tcaacctgcc
    11941 cttcgtgcac gtcctggtga acaacgccta cctcggcctg atccgccagt cccagcgcgc
    12001 gttcgacatg gacttctgcg tccagctgtc cttcgacaac atcaacgccc cggaggtcaa
    12061 cggctacggc gtcgatcacg tcaaggtcgc ggagggactg ggctgcaagg cgctgcgggt
    12121 cgtcgaaccg gccgagatcc tgccggcttt ggaggacgcg aagaagctca tggccgagca
    12181 ccgggtgccg gtcatggtgg aggtcatcct cgaaagggtc accaacattt ccatgggcac
    12241 cgaactcgac aacgtcgtcg agttcgagga cctcgccgcc agccacgagg acgcacccac
    12301 gttcgtcgca ctgctggact gatgggacgg cgccgccgta aagagggacg ccgcagtcga
    12361 ctcaggccga ccagccatcg gtgctgcggg ccgcgccccg ctgccgggat ccccggccga
    12421 gtctggtcag gatgcgtgga acccgccacg gcaccccccg cggcagcggc cggccgccgg
    12481 ccgccgccgc gagcaggtcc gcgaggtgga cggcacggat cgcgctgccc cggcgccgca
    12541 ggccggtctg cagctggcgc aggcagccgg ggttgaccgc gacgacgtag tcgacggcgg
    12601 cagcctcgaa cgcgtcgagc ttcgggtcga ggatccgccg gctgtcccgc gggcgcagca
    12661 gtgagtacgt gcccgcggag ccacagcacg agtcgtcgcc cgtcaccggg acgaactcgg
    12721 cgacggtcga gatcagcgcc cgcggctcgg cgaagacgcg cagcccgttg cggaggtggc
    12781 aggagtcctg gatcgcgacc cgcgcacggc gaccgtcgac ggtcacctct ccgacgggcg
    12841 ccgcgccggt cttggccagg tgctcgctga gctcgtgcac ccggtcgcgg ccgagcactc
    12901 cggccaggtg cgcggcgcag cccccagccg tggtcacgat gacgcccggg agctcctcac
    12961 ccagccgtcg ggccagctcg cgaccccgct cggactcgcc attgtgggcg tgcagtgctc
    13021 cgcagcagcc ctgctgcgcc ggcacagcga tgtcgggcag caacgtgcgg acggtccagc
    13081 tgagccccgg gtacaggccc cgctcgaagc agccgagcat gagcgaggtg tccgccggac
    13141 gcggtgccgt gcgggcgtgc cgccgcacta cgcccatcgc ccgcagcagc ggggtccgtt
    13201 cggccaccat catcagcacc tgtgcgagcg gcggccggtg ccgcccgcgc cactggtggt
    13261 cgcgccactc ctccagcagg tcgccgtact gcacaccggc cgggcagacg ggttcgcacg
    13321 cccggcagcc gaggcagaac gacgactcct cgcgcaaccg cggatcgtcc tcgtcgagcc
    13381 gcccggtctc gagcgcccgc atcagggtga tgcggccgcg cggcgaggag ccttcgtcct
    13441 gcgacaccgc gtaggtcggg caggccggca ggcagaaccc gcaggagatg cagcggtcga
    13501 gcagctgcgg gtcgaagatc ccgcgcccgg gagggctggt ccgatgggcc tcgttggtct
    13561 cgacgtggcc ggtcatgagc ccagcttccc ggggttgagg atcccggcgg ggtcgaacgc
    13621 ggccttgatg cggcgcagca gcgccatctg gtcggccccg agccgggcct gcaggtacgg
    13681 cagcttggcc gcgccgacac cgtgctcgcc ggtgatcgtg ccgtccatgt cgatcgcggc
    13741 cgcgaacacg tcgccgaacg ccttgtgcgc ccggtctatc gcggcctcgt cgcgctggtc
    13801 gaccacacag gtggggtgca ggttcccgtc gccggcgtgg ccgaacgtgg cgatcggcac
    13861 gtcgtaccgg tcggcgatgt cgtcgatgcg gtcgaccatc tcggccagcc gcgggcgggg
    13921 cacggtcgcg tcctccagca gtgtgagcgt cccgagccgc gacagcgcgg gcagcgagca
    13981 ccgccgcgcg gtgagcagtg cctccgagcg ggcgacgctc tcggcgagcg tcacctccat
    14041 cgccccgttg gccgtgcact gctcgccgat gcggccgagg ttgcggtcca cgacgtcggc
    14101 cgggccgtcg tcgccgaaca gcagcagcgc cccggcgtcc agccgcagcc cgaggttcgc
    14161 gtactcctcg accgcgccga tgcacttggc gtcgaggaac tcgagcgtgg cggggacgat
    14221 gccgtcgccg atgatcgccc cgactgcccg gccggcgtcg gccagcgtcg ggaagtaggc
    14281 gacgccggtg ttcgtcgtcg cgggcatcgg cagcagggcg acggtgacct cggtgaggac
    14341 cccgagggtg ccctccgagc cggtgagcag gcgggtgagg tcgtagccgg cgacgtcctt
    14401 ccacagccgc cctccggagc ggatcacctc gccggtcggg aggacggccg tcgcccccag
    14461 tacgtagttg cgggtgacgc cgtacttgag cccgcgcagc ccaccggcgc acgtagcgac
    14521 gttgccgccg acggtcgaga cggtgcggct gcccgggtcg ggcgcgtaca gcaggccctt
    14581 cgcggcagcg gcgtcggcca gcgcggcggt cgtgaccccg gcctcgacac gggcgagcag
    14641 ctcgtccggg ctgatctcca ggatccggtt catccgcgtg agcaccagca cgatcccacc
    14701 ccgctccgcc actgtggcgg cgcacaggtt cgagccggcg ccgcgcggca cgacggggac
    14761 gtgccgctgc gtggccaggc gcagcaccgc ggcgacctcc tccgaggtgc gcggccagac
    14821 gacggcgtcc ggcacggcgc ggaacaccgg cgtggcgtca cgggcgtagg gctccacgtc
    14881 gccgcggtcc gtgcggacgt gctcgccgcc gacgatcgcc tgcaactcgg cgaccaacgc
    14941 gcggtccagg ctcatggtcg gtctcctcct gcgtcgtcac cggtgacgac gagcacgtgc
    15001 agcgtgcgcg ggccgtgcac gccctcgacg cggtcgagct cgatgtcgct ggtcgcggac
    15061 ggccccgaga tccaggtcaa cgggcggtcg gagcggagtc gggcgacggc ggccggcacg
    15121 tcgtcgacca cgtcgtcggc ccgcacgacg cacacgtgga cgtcggggac gagcgtgagc
    15181 gctcgccggc cgcacagctc gccgctctcc agaacgatcg tcccggtctc ggcgatgccg
    15241 acgacggatc cggtgaccac cgcgtcgacg gcgtcgagct cggccaccga cagcggcgcc
    15301 tgccgggagt cctggaccag ggtgacggcc ccgtcgcccg gcacccattc cgtcggcagc
    15361 cccggcggca cggcaacgcg gctcgcgccg acggtgcgca gcgccttgtc caccgccgcg
    15421 gccacgccgt cggccgacac ctgctcgacg gtcgccttgt agtcggcgac ccgctcgcgg
    15481 aaccgctcga ccgtgccctc cggctgcacc cggctgcggt ggtagccgcg cgggatctcg
    15541 atgtcctcgt cggtcggcac gtcggtcagc gcggcgcgga tccggccgag cacctcctcg
    15601 cgcgctccca tcacgaggct cccttccggc tctgcttccc ccgcgtgcgc tgccaccagg
    15661 cccggaacga ctcccgcggg ggcaacggga tgtcgcgcgc ccggctccac ttcgccgccg
    15721 ggcccaggaa cgggatcggt cccagccccg cccggtcgcc gagcagccgg cttccgaacc
    15781 cggccagccg ctggaccgcg gcgaggcggc cgggccggtc gaacgtccac ccggccgccc
    15841 gcatgccgag catctcggcg ttcggcacgg ggtgctcccg cgaggcgtcg acgaccttgc
    15901 cacgcaggtg caccagcacg tccgggatgt cgatcgccac cgggcagacc tcatagcagc
    15961 gcccgcacag cgacgaggcg tacggcagcg acttctccac cggccccgcg tcgacgccgt
    16021 tgagcagcgg gttgaggatc gccccgatcg gacccgggta gaccgaaccg taggcgtgcc
    16081 cacccgtgcg ctcgtacacc gggcagaagt tcatgcaggc cgagcaccgg atacagcgca
    16141 gggcctgccg gcccacaggg tcggcgaggg ctcgcgtgcg cccgttgtcg accaggacga
    16201 tgtggacgct ctgcggcccg tcgccctcgg acacgcccgt ccacatcgag gtgtacgggt
    16261 tcatccgctc tcc
//
LOCUS       DASRSC010000177         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2026703,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000177 DASRSC010000000
VERSION     DASRSC010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2026703"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VFE39_08925"
     CDS             complement(<1..625)
                     /locus_tag="VFE39_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HET6258760.1"
                     /translation="MADQISDAILDAVLAADPASRVACETLITTGLVVVAGEITTTAT
                     VDFAEVVRRTICDIGYDNGAYGFDGRHCAVMVTLDKQSPDIAQGVDVAHEHRGRLSED
                     ELDSAGAGDQGMMFGFATRETPELMPLPIALAHRLSRRLSEVRKDGTLPYLRPDGKTQ
                     VTIEYAGDQAVRVDTVVVSSQHAQDVDLDRVMTGDIRQHVVEPELAEL"
     gene            complement(691..888)
                     /locus_tag="VFE39_08930"
     CDS             complement(691..888)
                     /locus_tag="VFE39_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258761.1"
                     /translation="MAAWMERSPRALRLFTTVERRSKEILITPDCGLLHVPAHAARAK
                     MLAMATAAAAVRDRIQKEKNT"
     gene            complement(985..1278)
                     /locus_tag="VFE39_08935"
     CDS             complement(985..1278)
                     /locus_tag="VFE39_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258762.1"
                     /translation="MDTTSRPGRDLRTGTEHTLVGPLLRELERIAGRADTERATADQE
                     LIAELRREIAALRAAVAHLRAGTSALPGHLRPVVARAMVGLDPAGGGASNAAG"
     gene            1415..2737
                     /locus_tag="VFE39_08940"
     CDS             1415..2737
                     /locus_tag="VFE39_08940"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6258763.1"
                     /translation="MPTSACPSAVPAAGDRRENLLLRELVAVYSHLSGLASQDSDVDG
                     VVRLVAHRTGSAVAVLGPTVEVLAAAPTDAGERLADPAVSASLGPVLAAAAQNRRPVS
                     LPGLGAAAGPIVVAPILVGDEVAAHLVTLPPVEPRSGEQELDDDLNLLLTEHAATICG
                     IILGRERVVAAAAGQARTDLIEGLLLARDRDDGEAERWAGHLGFVPGCEHHVLTVRLL
                     EGGGAGTPASDDTAHVLALAEHAISRRLPGAIVAVRDTEVVAVVAAPPGRGGGLDGVR
                     SLAEGCRAAVLDRHPDAAVVAGVGGCCRTAGEISRSYSEARSAVDAARRMGRAPAVLA
                     FDDLGVHRLLLQVPDLGELRAFAADVLGELVRDREHRANLVGTLSEWFRCNGSPQRTA
                     RELHVHPNTVSYRIRRVEEIAGLRLDHYRDRLMAQLACEIMQVLGDLS"
BASE COUNT          337 a          978 c         1069 g          443 t
ORIGIN      
        1 gcagctcggc cagctcgggt tccaccacgt gctggcggat gtcgccggtc atcacccggt
       61 ccaggtcgac gtcctgggcg tgctggctgg acaccaccac ggtgtccacc cggaccgcct
      121 ggtcaccggc gtactcgatg gtcacctgcg tcttcccgtc cgggcgcagg tagggcagcg
      181 tgccgtcctt gcgcacctcc gacaggcgcc gggagagccg gtgcgcgagc gcgatcggca
      241 gcggcatcag ctccggcgtc tcccgcgtgg cgaagccgaa catcatgccc tggtcgcccg
      301 ctccggcgct gtccagctcg tcctcgctga gcctgccgcg gtgctcgtgg gccacatcca
      361 cgccttgcgc gatgtcgggc gactgcttgt ccagggtcac catcacggcg cagtggcggc
      421 cgtcgaaccc gtaggcgccg ttgtcgtagc cgatgtcgca gatggtccgg cgaacgacct
      481 ccgcgaagtc gacggtggcc gtcgtcgtga tctcgccggc caccacgacc aagccggtgg
      541 tgatgagcgt ctcgcaggcg acgcgggagg cggggtcggc agcgagcacg gcgtcgagga
      601 tcgcgtcgga gatctggtcg gccatcttgt cggggtggcc ctcggtgacc gactccgacg
      661 tgaagaggtg ctcgttctcg gccagggtga tcacgtgttc ttctccttct ggatgcggtc
      721 gcggacggcg gcagccgcgg tggccatcgc gagcatcttt gcgcgggcgg cgtgggccgg
      781 tacgtgcagc aggccgcagt cgggggtgat caggatctcc ttgctccggc gttcgacggt
      841 ggtgaacagc cgcagggcac ggggcgagcg ctccatccag gcggccacgc gacggtagag
      901 ggcattgacc cggctcgctc ccggcgcgct gatcgtggtg gatagtcccg ctatttgtgg
      961 tggttcacag gcgcctggga cggctcatcc cgccgcgttc gacgcgcctc cacccgccgg
     1021 gtcgaggccg accatggctc gcgcgacgac gggccgcagg tgccccggca gcgcggaggt
     1081 gccggcgcgt agatgtgcga cggccgcccg cagggccgcg atctctctcc tgagttcggc
     1141 gatgagttcc tggtcagcgg tggcacgctc ggtgtcggcg cggccggcga tgcgctcgag
     1201 ctcgcggagc agcggcccga ccagggtgtg ctcggtgccg gtgcggagat cccgtccggg
     1261 cctcgaagtc gtgtccactc gtgctccttc gcatcggcgt cccgctcggc atccccgacg
     1321 ttatccaccc ggggaccccg gttacatgtg tcggaacaca aatcgtcgcc gtcggtcctt
     1381 ggggagatcg gacagtggcc attacgctgc tcccatgcct acgtcggcat gtccgtctgc
     1441 cgtcccggcc gcaggtgacc gccgggagaa cctgctgctg cgcgagctgg tggccgtcta
     1501 cagccacctg tccggcctcg cctcccagga ctccgacgtg gacggcgtcg tccggctcgt
     1561 cgcgcaccgc accggatcgg ccgtggcggt actcgggccc accgtcgagg tgctcgccgc
     1621 ggcgcccacc gatgcggggg agcggctggc ggaccccgct gtgtcggcct cgctcgggcc
     1681 ggtactggcc gccgcggcgc agaaccgcag gccggtgtcg ttgccagggc tcggtgccgc
     1741 ggccgggccg atcgtcgtgg cgccgatcct cgtcggcgac gaggtcgcgg cgcacctggt
     1801 gacgctgccg ccggtcgagc cgcggtcggg tgagcaggag ctcgacgacg acctgaacct
     1861 cctgctgacg gagcacgcgg cgacgatctg cgggatcatc ctcggccgcg agcgggtggt
     1921 cgcggccgcc gctggccagg cccgcaccga cctcatcgag gggttgttgc tcgccaggga
     1981 ccgcgacgac ggcgaggcgg agcgctgggc cggccatctc ggcttcgttc ccgggtgcga
     2041 gcaccatgtg ctcaccgtgc ggctactgga gggaggcggt gccggcacgc cggcgtccga
     2101 cgacaccgcc catgtgctcg cgctcgccga gcacgcgatc tcccggcggc tacccggggc
     2161 gatcgtcgcg gtccgggaca cggaggttgt ggccgtggtg gcagcccctc cggggcgcgg
     2221 tggcgggctc gacggtgtcc gctcgctggc cgaggggtgc cgggccgcgg tcctggaccg
     2281 ccatccggac gccgcggtcg tcgccggggt gggcggctgc tgccggacgg cgggggagat
     2341 ctcccggtcc tactccgagg cgcgcagtgc ggtcgacgcg gcgcgccgga tgggtcgggc
     2401 gcccgccgtg ctcgcgttcg acgacctcgg cgtgcaccgg ttgctgctgc aggtgcccga
     2461 cctcggggag ctgcgcgcct tcgccgctga cgtgctcggc gagctggtgc gcgaccggga
     2521 gcaccgggcc aacctcgtcg gcaccctgag tgagtggttc cgctgcaacg gcagcccgca
     2581 gcgcaccgca cgggagctgc acgtgcatcc gaacacggtg tcctaccgca tccggcgggt
     2641 cgaggagatc gccgggctca ggctggacca ttatcgcgac cggctgatgg cgcagctggc
     2701 gtgcgagatc atgcaggtgc tgggggacct atcgtgacca ggctgaacaa gtcgcccgcg
     2761 gggttccgcg agcgggtggc gcagggggtg ctcgtcgccg acggggcgat gggcacggtg
     2821 ctgcacg
//
LOCUS       DASRSC010000178         4140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3009481,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000178 DASRSC010000000
VERSION     DASRSC010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3009481"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VFE39_08945"
     CDS             complement(<1..624)
                     /locus_tag="VFE39_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020629370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6258764.1"
                     /translation="MTRTAPTRPPAATTGAAVSIRDVHRSYGGVRAVDGVDLAIAPGE
                     VVALLGPNGAGKSTTIDILLGLSLADSGDVRLFGRTPRRAVVDGLVGAMLQSGDIPDD
                     VTVAEVVGLTGALFPKPLPVAEALRRAGVADVAGRRYGRLSGGQKQRVRFAAALVCDP
                     DLLVLDEPTVAMDVRARREFWTAMRGYTRSGRTVLFATHYLAEAEEYA"
     gene            1041..2399
                     /locus_tag="VFE39_08950"
     CDS             1041..2399
                     /locus_tag="VFE39_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET6258765.1"
                     /translation="MTTFLSIMLGVLVVVAITALTGYFVAQEFGFMAVDRSRLKARAA
                     AGDAVAERTLGITRRTSFMLSGAQLGITVTGLLVGYVAEPMIGYGIGELLGGVGASPG
                     VGLAIGTVFALLFATVVQMVFGELFPKNLAISRSEPVARALTLSTSIYLTLFGWLIRL
                     FDAASNLLLKVLRIEPVHDVEHAATPRDLEAIIDESRDSGDLDDELSTLLDRVLDFTD
                     RTARAAMIPRPRVTSVSAEETVDHLVAVMASGHSRLPVIGSHADDVVGVICLRDVLAL
                     EGRDLATVRVADVARLPVFVPASLPLPSVLVRLRETDDEFAVVVDEYGGLAGVITIED
                     IAEELVGEITDEHDPAGVDRTRIGDGEWTVPGTMSVDEVERLLDADLPDGDYQTVGGL
                     LISELQRLPEAGETVIIRLPRVAGADDEEEPRRLAVTVCALERRVPATVRLAWAASEV
                     PA"
     gene            2396..3400
                     /locus_tag="VFE39_08955"
     CDS             2396..3400
                     /locus_tag="VFE39_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET6258766.1"
                     /translation="MNTGAAIAVSVGLIALSAFFVAVEFALVAARRYRLEEAATSSPA
                     ARAALRSAKDLSLLLAGSQLGITLCTLGLGAIGKPAVEDLLAPLFTGLGEGAATVMSF
                     VLSLVVVTFLHLVVGEMAPKSWAIAHPERSATMLALPMRAFMVVTRPVLVALNGMANW
                     CLRRVGVQPVDELGAGRNPDDLRELVDHSATTGALDAERHDRLVTALELDSTPLREVV
                     RPRAEAAGVPAGAGVEAIRAAARASGHLRLVVGDPDGRPIGVVHVRDALVQPAGTIAA
                     DLMRPVPTLDAATPIHDALGAMRERRNHLALVEADGELLGLVTLQDLLDRLFPAPSSG
                     "
     gene            3493..3693
                     /locus_tag="VFE39_08960"
     CDS             3493..3693
                     /locus_tag="VFE39_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258767.1"
                     /translation="MDASTLHPAELDDSALAKIRALEQQLGCPLVAYAPETPYAPLSD
                     EQVAALQRAEAELGVRLLAYHR"
     gene            complement(3709..>4140)
                     /locus_tag="VFE39_08965"
     CDS             complement(3709..>4140)
                     /locus_tag="VFE39_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HET6258768.1"
                     /translation="YVIEPAAGVGRSMMAFLIEAYTEDEAPNAKGGVDKRVVLRLDRR
                     LAPVKAAVLPLSRNADLSPKARDLAAQLRKNWNVEFDDAGAIGRRYRRQDEIGTPFCI
                     TVDFDTLTDQAVTIRERDSMAQERVALDQVEGYLAARLLGC"
BASE COUNT          553 a         1524 c         1465 g          598 t
ORIGIN      
        1 ggcgtactcc tccgcctccg ccaggtagtg cgtggcgaac agcaccgtgc gcccgctgcg
       61 cgtgtagccg cgcatcgccg tccagaactc gcgcctggcc cgcacgtcca tcgcgactgt
      121 cggctcgtcc agcacgagaa ggtccgggtc gcagacgagc gcggcggcga accgcacacg
      181 ctgcttctgc ccgcccgaga ggcgcccgta gcgccggccc gccacgtcgg cgacgcccgc
      241 ccggcgcagc gcctccgcga cgggcagcgg cttcgggaac agcgcgcccg tcaacccgac
      301 gacctccgcc accgtgacgt cgtccgggat gtcgccggac tgcagcatcg ccccgaccag
      361 cccgtcgacc accgcccgcc gcggcgtccg cccgaacagc cggacgtcgc cggagtcggc
      421 aagcgagagc ccgagcaaaa tgtcgatcgt cgtcgacttg ccggcgccgt tgggcccgag
      481 cagcgcgacc acctcgccgg gggcgatcgc gaggtcgacg ccgtcaaccg ctcggacccc
      541 gccgtagctg cgatgcacgt cccggatcga caccgccgca cctgtggtgg cggcaggggg
      601 acgcgtcgga gcggttctcg tcatgccgat gacactgtcg cccccgcccc gcccgcgccc
      661 cgatcacccg tcacgacctc cccgtgacgg atgtcaggga cgccccgtcg aacgaacggc
      721 actcgcgtcc gatgggtctg gccgaacgca ccgcccgtcc ggaagccggg ctcctgctcg
      781 cacccggctc cgcaccgatc accgggaccg agcggcccgt cgatcgggcg tcaggaggtc
      841 accgtgagcg atgtggcgca gtagccgaga atcgccgtac tgtgacgcac tcgcggccat
      901 tccgggaagc agccatcccg acggcgcgtt caacgtaacg agatgcccat ctgggctgcc
      961 tacagaacag gaccgatgga cccctccgac agtacgggca ccgtcctcgc aggacggtcc
     1021 cccgcccggg ccggtcgccc gtgaccacct tcctctcgat catgctgggc gtgctcgtgg
     1081 tcgttgcgat caccgcgctc accggctact tcgtcgccca ggaattcggc ttcatggccg
     1141 tggaccgttc tcggctcaag gcccgcgcgg ccgccggtga cgcggtcgcg gagcgcacac
     1201 tgggcatcac ccgccgcacc tctttcatgc tctcgggcgc acagctcggt atcaccgtca
     1261 ccgggctgct ggtcgggtac gtggccgagc cgatgatcgg ctacggcatc ggcgagctgc
     1321 tcggcggcgt cggtgcgtct cccggcgtcg gcctcgcgat cggcaccgtg ttcgcgctgc
     1381 tattcgccac cgtggtgcag atggtgttcg gggagctctt ccccaagaac ctggcgatct
     1441 cgcgctccga accggtggcg cgggcgctca cactgtccac gtcgatctac ctgacgctgt
     1501 tcggttggct gatcaggctg ttcgacgccg cgtcgaacct tctcctgaag gtgctgcgca
     1561 tcgagccggt gcacgacgtg gagcacgccg ccacgccgcg cgacctcgag gccatcatcg
     1621 acgagtcccg cgacagcggc gacctcgacg acgagctgtc cacgctgctc gaccgcgtcc
     1681 tcgacttcac cgaccgcacc gcgcgcgccg cgatgatccc gcggccgcgc gtgacgtcgg
     1741 tgtcggccga ggagacggtc gaccatctgg tggcggtgat ggcgtccggg cactcccggc
     1801 tcccggtgat cggcagccac gccgacgacg tcgtcggcgt gatctgcctg cgggacgtgc
     1861 ttgcgctgga gggccgggac ctggcgaccg tgcgcgtggc cgacgtggcc cgcctgccgg
     1921 tgttcgtgcc ggcatcgctg ccgctgccct cagtgctcgt ccggctgcgc gagaccgacg
     1981 acgagttcgc cgtcgtcgtc gacgagtacg gcggcctcgc cggcgtcatc accatcgagg
     2041 acatcgctga ggagctcgtc ggggagatca ccgacgagca cgacccagct ggcgtcgacc
     2101 gcacccggat cggcgacggg gagtggacgg tgccgggcac gatgagcgtc gacgaggtgg
     2161 agcgcctgct ggacgccgac ctgcccgatg gcgactacca gaccgtcggc ggcctgctga
     2221 tcagcgagct gcagcggttg ccggaggcgg gcgagacggt catcatccgg ctgccccgag
     2281 ttgcgggcgc cgacgacgag gaggagccgc ggcggctcgc cgtcacggtg tgcgccctcg
     2341 agcgccgggt gccggccacg gtacgcctgg cctgggccgc ctcggaggtg ccggcatgaa
     2401 caccggagcg gcgatcgccg tctcggtcgg cctgatagca ctcagtgcgt tcttcgtcgc
     2461 cgtcgagttc gcgctcgtgg cggcgcggcg ttaccgcctg gaggaggccg ccacgtcgag
     2521 cccagcggcg cgcgcggcac tgcgcagcgc gaaggacctc tcgttgctgc tcgccgggtc
     2581 gcagctcggg atcacgctgt gcacgctggg gctgggcgcc atcggcaagc ccgcggtcga
     2641 ggacctgctg gcgccgctgt tcaccgggct cggcgagggg gccgcgaccg tcatgtcgtt
     2701 cgtgctgtcg ctggtcgtgg tgacgttcct gcacctggtc gtcggtgaga tggcgccgaa
     2761 gtcgtgggcg atcgcgcacc ccgagcgctc ggcgacgatg ctggccctgc cgatgcgagc
     2821 gttcatggtc gttacgcggc cggtgctggt ggcgctcaac ggcatggcga actggtgcct
     2881 gcgccgcgtc ggcgtgcagc cggtggacga gctgggagcc gggcggaacc ccgacgacct
     2941 gcgcgagctg gtggaccact cggctaccac aggcgcgctg gacgcggagc gccacgaccg
     3001 gcttgtcacg gcgctggagc tcgacagcac cccgctgaga gaggtggtca ggccccgcgc
     3061 ggaggcggca ggcgtgccgg ccggcgccgg ggtggaagcg atccgggcgg cggcacgcgc
     3121 gagcgggcac ctgcggctcg tggtcggcga tccggacggc cgccctatcg gggtcgtgca
     3181 cgtccgcgac gctctcgtcc agcccgccgg caccatcgcg gccgacctga tgcggccggt
     3241 gccgacgctc gacgccgcca cgccgatcca cgacgcgctc ggtgcgatgc gggagcggcg
     3301 caaccacctg gcactggtcg aggccgacgg cgagctgctc ggcctcgtca cgctgcagga
     3361 cctgctggac cggctgttcc cggcgccgtc gagcggttga cgcgtccggg acccggctgg
     3421 gtggttctgc tcgccgggtc ccggggccgg tcctacgatc ggccaccacc gccccacggg
     3481 aggccgaacc cgatggatgc ctcgacgctc caccccgccg agctcgacga ctccgcgttg
     3541 gcgaagatcc gcgcgctgga gcagcagctg ggctgcccgc tcgtcgctta cgcaccggag
     3601 accccgtacg ccccgctcag cgacgagcag gtggccgcgc tgcaacgcgc ggaggccgag
     3661 ctgggcgtgc ggctgctcgc ctaccaccgc tagccccgag cccgctcctc agcagccgag
     3721 cagtcgggcc gcgaggtagc cctccacctg gtccagcgcc acccgctcct gcgccatgga
     3781 gtcgcgctcg cggatcgtga ccgcctggtc cgtgagggtg tcgaagtcga ccgtgatgca
     3841 gaacggtgtg ccgatctcgt cctggcgccg gtaacggcgg ccgatcgcgc ctgcgtcgtc
     3901 aaactcgacg ttccagttct tgcggagctg ggcggccaga tcgcgggcct tgggcgacag
     3961 gtcggcgttg cgcgacagcg gcagcaccgc agccttgacc ggcgcgagcc gccggtccag
     4021 ccgcaacaca acacgcttgt cgacgccccc cttggcgttg ggcgcctcgt cctcggtgta
     4081 ggcctcgatg aggaacgcca tcatcgacct gccgaccccg gcggcgggct cgatcacgta
//
LOCUS       DASRSC010000179         9241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2683009,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000179 DASRSC010000000
VERSION     DASRSC010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9241
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2683009"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1431
                     /gene="lnt"
                     /locus_tag="VFE39_08970"
     CDS             <1..1431
                     /gene="lnt"
                     /locus_tag="VFE39_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET6258769.1"
                     /translation="WAVLHGRGIRAGFGWGFVFGLSFLLPLLTWIGSLVGPVPWVALC
                     VFEALFVGLAGAGIALVSRLPAGPLWAAAVWVGGEALRSRVPFGGMPWGRVGFGQPDG
                     PLLPVASIGGVPLLSFATVLAGLALGELVRRIVRDPGVVRRSPLRLVGPAAVLVLTLV
                     AGPLAALVPATPGAPDRTATIAAVQGNVPRLGLDFNAQRRAVLDNHVRVTEQLAADVA
                     AGRRPQPDIVIWPENSSDIDPLQNPDAAAQIDRAARAIKAPILVGAVLRNPDGLTTTN
                     SSLVWEAGLGVVERNDKRRLQPFGEYMPWRSFFRHLSSYVDRAGYFVPGPGPGAVDMA
                     GIRVGIAICWEVAFDDLVEQSVDAGATVLAVPSNNATFGFSEMTYQQLAMSQVRAVEH
                     DRSVIVATTSGVSATITPDGTVTASTREFTPDVLVDRTTLRDTTTLATRLGSVPELVL
                     VALGVVAVGVGVVTRRRTGTGKDEDD"
     gene            1424..2197
                     /locus_tag="VFE39_08975"
     CDS             1424..2197
                     /locus_tag="VFE39_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HET6258770.1"
                     /translation="MTEPTGPVLVVIPTYDERDNLGPVLSRLHAAVPAADVLVVDDAS
                     PDGTGELADAMAADDPRIRVLHRAEKSGLGAAYLAGFAAALRGDHQVIVEMDADGSHA
                     PEDLPALLAALEDADLVLGSRYVPGGTVCNWPAHREWISRGGNLYSRIALGVSIQDIT
                     GGYRVFRRQVLEELDLDDVSSQGYCFQVDVAWRAVRAGFRVREVPITFTDRERGTSKM
                     SGGIVGEAFWRVTCWGANRLLHRSRGQDDAVSGARFVHD"
     gene            2190..2687
                     /locus_tag="VFE39_08980"
     CDS             2190..2687
                     /locus_tag="VFE39_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HET6258771.1"
                     /translation="MTEVREELTWELFGTASRELAEQVAADGYQPDIILSIARGGLFV
                     AGALGYALDVKNLHVANVEFYTGVNERLPVPILLPPVPNVVDLSGAQVLVADDVADTG
                     ATLKLVRDFCAGHVADVRCAVVYEKPHSTVACEYVWRHTDRWINFPWSTQPPVVKRSN
                     QVLDA"
     gene            complement(2768..3112)
                     /locus_tag="VFE39_08985"
     CDS             complement(2768..3112)
                     /locus_tag="VFE39_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein RbpA"
                     /protein_id="HET6258772.1"
                     /translation="MADRVLRGSRLGAVSYETDRNHDLAPRQSMRYACPRGHDFEVPF
                     ANDAEAPATWECRLHGSISRLVDGNEPEQKKVKPPRTHWDMLLERRSVAELEALLTER
                     LELLAERRREIA"
     gene            complement(3441..4745)
                     /locus_tag="VFE39_08990"
     CDS             complement(3441..4745)
                     /locus_tag="VFE39_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6258773.1"
                     /translation="MNTAAPPTSAARGQIVSWGLWDWGSAAFNAVILTFVFSVYLTDA
                     VGEDLPGSVNANSWLGYSIGASGLLIALMAPVIGQRTDAAGRRKLSVGIWTALTVASM
                     AGLSLVEDDYRFLWLGLLLLGLGSIFFELASVSYNAVLTQVSTPATIGRVSGFGWAMG
                     YFGGIVLLLVVYVGFISGDGGILGVPTENGLNIRLVALVAAGWFLVFALPMFFAVPEA
                     PPRTARPPRLGVVGSYRALFSDLRDLYRSSPHTVYFLGASALFRDGLAAIFTFGAVLA
                     VTVYGIGAGDVLVFGVAANVVSAIGALVAGRIDDRVGPKAVIVGSLVGMLVAGVILLF
                     VSGPTMFWIFGLFLTLFVGPAQSSSRTFLARLAPAGQEGQLFGLYATTGRAVSFLAPT
                     LVGLFTYLFGSDRAGIVGILLVLAVGLLALSPVRPPVVAADA"
     gene            complement(4808..5605)
                     /locus_tag="VFE39_08995"
     CDS             complement(4808..5605)
                     /locus_tag="VFE39_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HET6258774.1"
                     /translation="MDRHPYLDGPYPRAYAHRGWHTGDLAGYENTLPAFRRAVDEGFG
                     YLELDVHASADAVPVVHHDATLDRTTDGVGPIAALPAAAIAQARVNGSEPIPLLEQVL
                     TELPDTRITIELKSHAVVEPVLAVLDRTDSWHRVCLGSYHDGRLAKARSAGGARLCTS
                     LAQRSAFGLRARAWLDALPGPLSRLPAPPVTGNLAQLPRRFGALTVVDAALVRAAHQG
                     GREIHVWTVDDPVEMGALLDLGVDGLLSDRPDLLREVLHSRGSWQGS"
     gene            complement(5632..7554)
                     /gene="ligD"
                     /locus_tag="VFE39_09000"
     CDS             complement(5632..7554)
                     /gene="ligD"
                     /locus_tag="VFE39_09000"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HET6258775.1"
                     /translation="MPLEEYRRKRDARRTPEPVPETDPTSPGPGHRFVIQEHHARRLH
                     WDVRLERDGVLASWAVPKGLPTEPETIRLAVRTEDHPIEYLEFSGEIPPGEYGGGAMT
                     IWDAGTYETEKWNPREVIVRLRGDRASGRFVFIRTDRDGAKDNWLLRRSDPAPGREPL
                     PHGVLPMLATPGPPPTADDNRDRWLQVGFGGRRVMLRVEGGRTQVTDEDGEELSVPRL
                     RELGPSLGATQVLLDGELTGPVEKAELWIGDLLHLDGRDTCRLPFRERRALLERLSLD
                     GQHWKLAPVFPGGGPEVVAAAGQQGLPHVVAKDADSPYEPGRRSARWVEVSTGAPAKQ
                     GRREAPRRGGAAGVGRARLTNPDKVLYPLTGTTKSEVLAHYLAVADVMLPHLRNRAVT
                     MVRWPDGVEKSSFFEKDVSRHAPEWIRTARVGTPGGRSENADFPLIDDAEGLAWAANL
                     AALELHVPQWRIGPRGGQQLPDLVVFDLDPGEGTTVVDCARVAERIADRLADDELVAY
                     PRTSGGKGLQLYLPVTVSRAERTSEFAKALAEELARETPGRVTAVMAKARRRGKVFVD
                     WSQNNPHKTTIASYSLRGRARPTVATPVTWEEVRSCRRVEDLVFTADDLPARIAEHGD
                     LLAPLFTDPQRLPRRS"
     gene            7627..7824
                     /locus_tag="VFE39_09005"
     CDS             7627..7824
                     /locus_tag="VFE39_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET6258776.1"
                     /translation="MYVCICAAVPDTEIRSCIARGARTVEDVGNACGAGTGCGSCHDH
                     IDVFLAAAHAPSELMDLPATA"
     gene            7931..8416
                     /gene="bfr"
                     /locus_tag="VFE39_09010"
     CDS             7931..8416
                     /gene="bfr"
                     /locus_tag="VFE39_09010"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HET6258777.1"
                     /translation="MRGDPEIIALLNDQLTSELTAINQYFLHAKMQQNWGLTKLAAYT
                     RAESIDEMRHAEKITDRILFLEGLPNYQRLFSLRIGQTVREQLQADLAVELEVVERLQ
                     PGVTMCREKGDHTTANLFEEILADEEHHIDYIETNLELIDRLGEQLYLAQLVDQPPTP
                     G"
     gene            8758..8970
                     /locus_tag="VFE39_09015"
     CDS             8758..8970
                     /locus_tag="VFE39_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3072 domain-containing protein"
                     /protein_id="HET6258778.1"
                     /translation="MTENRAGADSSAAKDPDDWTTGDEPMTGAQKSYLQTLAQEAGEQ
                     PPPDDMSKADASKRIDELQERTGRGR"
BASE COUNT         1413 a         3206 c         3273 g         1349 t
ORIGIN      
        1 tgggcggtgc tgcacggtcg cggcatccgc gcagggttcg gatggggctt cgtgttcggg
       61 ctctcgttcc tgctgcccct gctcacctgg atcggctctc tcgtgggccc cgtgccgtgg
      121 gtggccctgt gcgtgttcga agcgctcttc gtggggctgg ccggggccgg gatcgcgctg
      181 gtgtcccggc tgccggccgg gccgctctgg gcagcagcgg tgtgggtcgg gggtgaggcg
      241 ctgcggtcgc gggtgccgtt cggcgggatg ccgtgggggc gcgtcgggtt cggccagccc
      301 gacgggccgc tgctgccggt ggcgtcgatc ggcggggtgc cgttgctgtc gttcgccacc
      361 gtgctcgccg ggctggcgct cggggagctg gtgcgccgga tcgtccgcga tccgggtgtc
      421 gtacgacgta gtcccctccg gctggtcggg ccggcagcgg tcctcgtgct gacgctggtg
      481 gccggtccgc tcgccgcgct cgtacccgcc acccccggag cgcccgaccg caccgccacg
      541 atcgccgccg tgcagggcaa cgtgccgcgc ctgggcctgg acttcaacgc ccaacgccgg
      601 gcggtgctcg acaaccacgt ccgggtcacc gagcagctgg ccgccgacgt cgcggccggg
      661 cggcgtcccc agccggacat cgtgatctgg ccggagaact ccagcgacat cgaccccctg
      721 cagaaccctg acgccgccgc ccagatcgac cgtgcggcgc gcgccatcaa ggccccgatc
      781 ctggttgggg ccgtgctgcg caacccggac gggctcacca cgaccaactc ctcgctcgtc
      841 tgggaggcgg gcctcggggt ggtcgagcgc aacgacaagc gcaggttgca gcccttcggt
      901 gagtacatgc cgtggcgcag cttcttccgg cacctctcgt cgtacgtcga ccgggccggg
      961 tacttcgtgc cgggcccggg tccgggcgcg gtcgacatgg ccgggatccg ggtcggcatc
     1021 gcaatctgct gggaggtggc gttcgacgac ctggtcgagc agagcgtcga cgccggcgcc
     1081 accgtgctcg ccgtgccgag caacaacgcc accttcgggt tctcggagat gacgtaccag
     1141 cagctcgcaa tgtcgcaggt ccgggccgtc gagcacgacc gttcggtgat cgtggccacc
     1201 acgagcggtg tgagcgcgac catcactccc gacggcaccg tcacggccag cacacgcgag
     1261 ttcactcccg acgtgctggt cgatcgaacc actcttcggg acaccactac gctggccacg
     1321 cggctgggat ccgtacccga gttggtcctg gttgcgttag gagtggtggc ggtcggtgtc
     1381 ggggttgtga cccgacgtcg cacgggcacg gggaaggacg aggatgactg agcccacggg
     1441 gccggtgctg gtggtgatcc ccacctacga cgagcgggac aacctcggtc cggtgctgtc
     1501 gcggctgcat gctgcggtac cggcagctga cgtcctcgtg gtggacgacg cgagccccga
     1561 cggtacgggc gagctcgccg acgcgatggc tgcggacgat ccccgaatcc gggtgctgca
     1621 ccgcgcggag aagtccggcc tcggtgcggc ctacctcgcg ggtttcgccg ccgccctgcg
     1681 tggcgaccac caggtcatcg tggagatgga cgccgacggc tcgcatgctc ccgaggatct
     1741 gccggccctg ctggccgccc tcgaggatgc cgatctcgtg ctgggctcgc gctacgtgcc
     1801 cggcggcacg gtgtgcaact ggccggcgca ccgcgagtgg atctcccgcg gcggcaatct
     1861 gtactcgcgc attgcgctgg gcgtgtcgat ccaggacatc accggtggct accgtgtgtt
     1921 ccgccgccag gtgctggagg agctcgatct cgacgacgtg tcgtcgcagg gctactgctt
     1981 ccaggtcgac gtggcgtggc gggccgtgcg ggccggattc cgggtccgcg aggtacccat
     2041 cacgttcacc gatcgcgagc ggggaacgtc gaagatgagc ggcgggatcg tcggcgaagc
     2101 gttctggcgt gtgacgtgct ggggggccaa ccgactgctc caccgctccc ggggccagga
     2161 cgacgcggtg tccggggcgc ggttcgtgca tgactgaggt ccgcgaggag ctgacctggg
     2221 agttgttcgg gacggcgtcg cgcgagctgg ccgagcaggt cgcggctgac ggctaccaac
     2281 ccgacatcat cctgtcgatc gcgcggggcg ggctgttcgt ggccggcgcg ctgggctacg
     2341 ccctggacgt caagaacctg cacgtcgcca acgtcgagtt ctacacgggg gtgaacgagc
     2401 gcctgcccgt gccgatcctg ctgccaccgg tgcccaacgt cgtggacctg tccggcgcgc
     2461 aggtgctcgt cgcggacgat gtggccgaca caggggccac tctgaagctc gtccgcgact
     2521 tctgcgccgg tcacgtggcc gacgtgcgct gcgccgtggt gtacgagaag ccgcacagca
     2581 cggtggcgtg cgagtacgtg tggcggcaca ccgaccggtg gatcaacttc ccgtggtcga
     2641 cgcagccgcc ggtcgtgaag cggtcgaatc aggtgctcga cgcctagcag ggtgctgttc
     2701 agcgcgctca tcggcgcaca cgacaaagcc gggccacccc gcgggtgacc cggctccggt
     2761 tgtcaggtca ggcgatctcg cgacggcgct cggccagcag ctccagacgc tcggtgagga
     2821 gcgcctccag ttcggccacc gaccggcgct cgaggagcat gtcccagtgg gtgcggggtg
     2881 gcttgacctt cttctgctcg ggctcgttgc catcgaccag cctggagatg ctgccgtgca
     2941 acctacactc ccaggtcgcc ggcgcctccg catcgttggc gaaaggcacc tcgaagtcgt
     3001 gcccacgcgg gcacgcgtag cgcatcgact gccgcggcgc gaggtcgtgg ttgcggtcgg
     3061 tctcgtagct gacagccccg agccggctgc cacggagcac gcggtcggcc atgcgtcttc
     3121 ctcccgttca cggccccagg gaggttccct tggggcattt ccaccgggtg taacgtctgc
     3181 ggagcagccg tctgttcccg ccattggggc ggtccccgcc agatggcgtc gttgcccgac
     3241 ggacaccgga cgtttgttgc cccgatcgtt gagtgtcccc catgtaggcc cgttacacac
     3301 gccactgtga cgtgtcagcc gactgtgacc tatgtcaccg ctgttgacga ggtctttacg
     3361 tgggggcggc tccccgtcat cagaaccttc cccgcacgca aactgccggc caggacagtg
     3421 cagcgaggag cgcgtcgtcg tcatgcgtcg gccgctacga ccggcggacg caccggcgac
     3481 agcgccagca gacccacggc gagcaccagc aggatgccga cgatgccggc gcggtcggag
     3541 ccgaacaggt aggtgaacag gcccacgagt gtgggggcga ggaaggacac cgctcgcccg
     3601 gtggtggcgt agaggccgaa cagctggccc tcctgacccg cgggggcgag ccgggcgagg
     3661 aaggtgcgcg acgaggactg cgcaggcccg acgaagagcg tgaggaacag gccgaagatc
     3721 cagaacatcg tgggaccgga cacgaacaag aggatcaccc cggccaccag catccccacc
     3781 agcgagccca cgatcacggc cttgggtccg acccggtcgt cgatccgccc cgccacgagc
     3841 gcgccgatcg cggagaccac gttggccgcg acaccgaaga cgagcacgtc gcctgcgccg
     3901 atcccgtaga ccgtgacagc caggaccgcc ccgaaggtga agatcgcggc gaggccgtcg
     3961 cggaacaggg cgctcgcgcc gaggaagtac acggtgtgcg gactggaccg gtagaggtcg
     4021 cgcaggtcgg agaacagtgc ccggtaggag ccgacgaccc ccaaccgtgg tgggcgcgcc
     4081 gtccggggcg gagcctcggg cacggcgaag aacatcggca gcgcgaagac gaggaaccag
     4141 ccggccgcga cgagcgcgac cagccggatg ttgagcccgt tttcggtggg gacgccgagg
     4201 atgcccccgt caccgctgat gaagccgacg tagaccacca gcagcagcac gatcccgccg
     4261 aagtaaccca tggcccagcc gaagcccgac acccggccga tcgtcgccgg cgtcgagact
     4321 tgggtgagca ccgcgttgta ggacaccgag gcgagctcga agaagatcga cccgaggccg
     4381 aggagtagca agcccagcca caggaagcga tagtcgtcct cgacgaggga gagcccggcc
     4441 atcgacgcga cagtcagcgc ggtccagatc ccgaccgaca gcttgcggcg ccccgctgcg
     4501 tcggtgcgct gcccgatcac gggggccatc agcgcgatga gcagacctga tgcgccgatc
     4561 gagtagccga gccagctgtt cgcgttgacg ctgccgggca ggtcctcacc caccgcgtcg
     4621 gtcaggtaca cggagaagac gaacgtcagg atcacggcgt tgaacgccgc cgatccccag
     4681 tcccacagtc cccaggagac aatctggccg cgagcggcag aagtgggcgg cgcggccgtg
     4741 ttcacgagcg gagggtagcg gcgaccgctg cccgtgcacg gccgtcctca acggtgatgc
     4801 tcaaccgtca cgagccctgc caggagccgc gggagtgcag cacctcgcgc agcaggtcgg
     4861 gccggtcgga gagcaggccg tcgacgccga ggtcgagcag ggcgcccatc tcgaccgggt
     4921 cgtcgacggt ccacacgtgg atctcgcggc caccctgatg tgccgcacgc acgagcgcgg
     4981 cgtcgaccac tgtcagcgca ccgaagcggc gggggagctg ggcgaggttg cccgtcaccg
     5041 gtggcgccgg taggcgcgac aacggccccg gcagcgcgtc gagccaggcc cgagcgcgca
     5101 acccgaatgc cgagcgctgg gccagggagg tgcacagccg ggcgccgccc gcgcttctcg
     5161 ccttggccaa ccgcccgtcg tggtagctcc cgaggcagac gcgatgccag ctgtcggtcc
     5221 ggtcgagcac cgcgagcacg ggctcgacga cggcgtggga tttcagctcg atcgtgatcc
     5281 gggtgtcggg cagctcggtg agcacctgtt cgagcagcgg gatcggctcg ctgccgttca
     5341 cccgggcctg ggcgatcgct gcggccggca gggccgcgat cggcccgacg ccgtcggtgg
     5401 tgcggtcgag cgtcgcgtcg tgatgcacga cgggcacggc gtctgcactc gcgtgcacgt
     5461 cgagctcgag gtagccgaac ccctcgtcga cagcgcggcg gaacgcgggg agggtgttct
     5521 cgtagcccgc gaggtcgccc gtgtgccagc cccggtgggc gtacgcccgc gggtacgggc
     5581 cgtcgaggta gggatgccgg tccacgcgtg gagcctgcca gccgtcgtgg ctcacgagcg
     5641 acgcggaagt cgttgcgggt cggtgaacag cggggcgagc aggtcgccgt gctcggcgat
     5701 ccgggcgggc aggtcatcgg cggtgaacac caggtcctcg acgcggcgac aggagcgcac
     5761 ctcctcccat gtcacgggcg tcgccacggt gggccgcgcc cggccccgca aggagtagct
     5821 tgcgatcgtc gtcttgtgcg ggttgttctg gctccagtcg acgaacacct tgccccggcg
     5881 tcgagccttg gccatcacgg cggtgacccg gcccggggtc tcgcgggcca gctcctcggc
     5941 gagcgccttg gcgaactccg acgtccgctc ggcacgggac accgtgaccg ggaggtacag
     6001 ctgcaacccc ttgccgccac tggtgcgcgg gtacgccacc agttcgtcgt cggccaggcg
     6061 gtcggcgatc cgctccgcga cacgggcgca gtcgacgacc gtggtgccct cacccgggtc
     6121 gaggtcgaac accaccaggt ccgggagctg ctgcccgccg cgcgggccga tccgccactg
     6181 cgggacgtgc agctccagcg cggcgaggtt cgcggcccac gccaggccct ccgcgtcgtc
     6241 tatcaacggg aagtcggcgt tctccgagcg gcctccgggc gtgccgaccc gtgcagtccg
     6301 gatccactcc ggggcgtggc gcgagacgtc cttctcgaag aacgacgact tctccacccc
     6361 gtccggccag cggaccatcg tgaccgcccg gttgcgcagg tgcggcagca tgacgtcggc
     6421 aacggccagg tagtgcgcga gcacctccga cttcgtggtg ccggtgagcg ggtagagcac
     6481 cttgtccggg ttcgtgagcc tcgcgcgccc gacgccggcc gctccgccgc gccgaggcgc
     6541 ctcccggcgc ccctgtttcg ccggtgcgcc cgtgctcacc tccacccacc gggcgctgcg
     6601 ccgccccggc tcgtacgggg agtcggcgtc cttcgccacg acgtggggga ggccctgctg
     6661 cccggccgct gccacgacct ccgggccgcc gccggggaag acgggcgcga gtttccagtg
     6721 ctggccgtcg agggatagcc gttccagcaa cgcgcgccgc tcccggaacg gcagccggca
     6781 ggtgtcgcgg ccgtcgagat ggagcaggtc tccgatccac agctcggcct tctccaccgg
     6841 ccctgtcagc tcaccgtcga ggagcacctg cgtggcgccg agcgagggcc ccagctcgcg
     6901 caggcgcgga acggacagct cctcgccgtc ctcgtcggtg acctgcgtcc ggccgccctc
     6961 cacgcgcagc atgacgcgcc tgcctccgaa accgacctgc aaccaccggt cccggttgtc
     7021 gtccgcggtc gggggcggcc cgggcgtcgc aagcatcggc agcacgccgt gggggagggg
     7081 ctctcgtccg ggagccgggt cggagcggcg cagcagccag ttgtccttgg ctccgtcacg
     7141 gtcggtacgg atgaacacga accggcccga cgcccggtca ccacgcagcc ggacgatgac
     7201 ctcgcggggg ttccacttct cggtctcgta ggtgccggca tcccagatcg tcatcgcgcc
     7261 gccgccgtac tcgccgggcg ggatctcccc ggagaactcc aggtactcga tcggatggtc
     7321 ctcggtacgc accgcgagcc ggatcgtctc cggctcggtc ggcaggccct tcggtacggc
     7381 ccaggaggca agcacaccat cccgctcgag ccggacgtcc cagtgcaacc ggcgcgcgtg
     7441 gtgttcctga atgacgaatc ggtgaccggg accgggcgac gtcggatccg tctcgggcac
     7501 aggctcgggc gtgcgcctcg cgtcgcgctt gcggcggtac tcctccagcg gcacgaagca
     7561 aggttagggc ttgcgtccac catccggacg atgtcagcat aggcttgcct gaacaaagga
     7621 gggcctgtgt acgtctgcat ctgcgcagcg gtgccggaca ccgagatccg ttcctgcatc
     7681 gcccgcggtg cccgtaccgt cgaagacgtc ggcaacgcgt gcggcgccgg caccggatgc
     7741 ggttcgtgcc acgaccacat cgacgtcttc ctcgcggccg cgcacgctcc gtcggaactg
     7801 atggacctcc ccgccacagc ctgagccctg aggatctcct gagctgcgca aaggcgatcc
     7861 tgctcggccc ccaacgcgca tcaccgctac cgtctgttgt ccctgactcc gacgaagcga
     7921 ggactgcggc atgcgtggcg accccgagat catcgccctg ctcaacgacc agctcacgag
     7981 tgagctgacc gcgatcaacc agtacttcct gcacgcgaag atgcagcaga actggggcct
     8041 caccaagctc gcggcgtaca cccgtgcgga gtccatcgac gagatgcggc acgcggagaa
     8101 gatcaccgac cgcatcctgt tcctcgaggg actcccgaac taccaacgcc tgttctcact
     8161 gcggatcggt cagacggtcc gcgaacagct gcaggccgac ctcgccgtcg agctcgaggt
     8221 cgtggagcgg ctgcagcccg gagtgacgat gtgccgggaa aagggtgacc acaccacggc
     8281 caacctgttc gaggaaatcc tcgccgacga ggagcaccac atcgactaca tcgagaccaa
     8341 cctcgagctg atcgacaggc tcggcgagca gctctacctg gcccagctcg tcgaccagcc
     8401 ccccaccccg ggctgactcc cggtccttca agaccagcca tccggttctg cttggcaccg
     8461 cctgaaaggg tttgcagagt ttggtcagcg acgacgggga gcggcatggc ggcgacaatc
     8521 tgatggcggc aacgatgtga tggcggcaac gatgtgatgg cggtgaacca tccgccacgt
     8581 ggcccaggcc gcggaagtct tcggtgtcga cggtatcccc ggcgatcacc gggcccgaga
     8641 tggtgcgccg agaaacccgc cggaaaaagg tgttgcaggc ggaggccgcc gcgctgtcat
     8701 aacgtcatgg ggacggctcc gccggcgagc ccgggggctc gaatgtatgt tcgagtcatg
     8761 accgagaacc gtgcaggggc agattccagc gccgccaagg atcctgacga ctggacgacc
     8821 ggcgacgaac cgatgaccgg cgcccagaag tcgtacttgc agacgctggc gcaggaggca
     8881 ggcgaacagc cgccaccgga cgacatgtcc aaggcggacg cctcgaagcg gatcgacgaa
     8941 ctgcaggaac gcaccggccg cggccgctga ctgaccgacc ggcactgggt gcttcccccg
     9001 ggtcgataac cgatgagccg ataatcaacg ttatgacagt tcctccgcgt caccgggcga
     9061 cgcccgccga tccagcggcc ctggtcggcg ggcctgctag aacgtgatct ccgggcctct
     9121 ctgccgacgc cggggttccc gaacgtccag cggctcctcg aggagtgcgc gaacgccgcg
     9181 aactgacccg agcacgaagg aggccccgca ccggcggtgt gggccccttc cgtgtgggtc
     9241 a
//
LOCUS       DASRSC010000180         1644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_61409,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000180 DASRSC010000000
VERSION     DASRSC010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61409"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VFE39_09020"
     CDS             complement(<1..846)
                     /locus_tag="VFE39_09020"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HET6258779.1"
                     /translation="MAAEEHAREVLQRVFGYDAFRGSQQQVIDHVVAGGDALVLMPTG
                     GGKSLCYQIPALVREGVGVVISPLIALMQDQVDALRALGVRADFLNSTQSLDERRAVE
                     ASFLAGDLDLLYLAPEGLSVPATQRLLDRGRIALFAIDEAHCVSQWGHDFRPDYLALA
                     MLHERWPTVPRIALTATATQATHAEIALRLNLKEARHFVASFDRPNIQYRIVPKNEPR
                     RQLLDLLRTEHRGDAGIVYCLSRASVDKTAEFLVQQGIAALPYHAGLDARTRAANQSR
                     FLREDC"
     gene            1060..>1644
                     /locus_tag="VFE39_09025"
     CDS             1060..>1644
                     /locus_tag="VFE39_09025"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET6258780.1"
                     /translation="MVRIRLLGPVDVIVHDTPYAVPGLRRKAVLVALALQPGQIVHVD
                     RLIDIVWGDAAPATAANTLQAHISQLRRLLGGRHTIQSRPPGYVLDVGAEATDLEQAE
                     RLLREGTQAADPVDGVALVRAAVSLWRGAPLADLAELPWFGDHARRLEHLLLHARQTL
                     VEARLALGEHTALVPELEALSRQHPLHEQIHRQLM"
BASE COUNT          244 a          564 c          591 g          245 t
ORIGIN      
        1 gcagtcctcg cgcaggaacc gcgactggtt ggccgcacgg gtgcgcgcgt cgagcccggc
       61 gtggtaaggc agggccgcga tgccctgctg cacaaggaat tcggcggtct tgtcgaccga
      121 cgcccgggac aggcagtaca cgatgccggc gtcgcccctg tgctcggtgc gcagcaggtc
      181 gagcagttgc ctgcggggct cgttcttcgg cacgatccgg tactggatat tgggccggtc
      241 gaagctcgcc acgaagtggc gagcctcctt caggttgagg cggagcgcga tctcggcatg
      301 ggtggcctgc gtcgcggtcg cggtcagagc gatccggggt accgtcggcc agcgctcgtg
      361 cagcatcgcc agtgccaggt agtcggggcg gaagtcgtgg ccccactgcg acacacagtg
      421 cgcctcgtcg atcgcgaaca gggcgatcct gccgcgatcc agcagccgct gcgtggccgg
      481 gacactcaga ccctccggcg ccagatagag cagatcgaga tcgcccgcga ggaacgacgc
      541 ctcgacggcg cggcgctcgt cgaggctctg tgtcgagttg aggaagtcgg cccggacgcc
      601 gagtgcccgc agtgcgtcga cctgatcctg catgagcgcg atcagcgggg agatgacgac
      661 gccgacgccc tcgcgcacca gggccgggat ctggtaacac agcgacttgc caccgccggt
      721 cggcatgagc acgagcgcgt cgccacccgc gacgacatgg tcgatgacct gctgctggga
      781 gccccggaag gcgtcatatc cgaagacccg ctggaggact tctcgcgcgt gctcctcggc
      841 tgccacccgg cgagtctacg ttcgcgaccg cgtgccgcaa ccacgccggt acgcgggtcc
      901 caaaagtccg gtgcatgact ttccgacctg gtcggctggg ggcaccgcta gcctcaggct
      961 ggttgggccc tgtcctgtcg atcgtgtcga gccgaggcga agtcggggcc tcgatccggc
     1021 aagacggcgg gacagggcct cgttgtggga gaaggtccgg tggtacggat aaggcttctc
     1081 ggtccggtcg acgtgatcgt gcacgacacc ccgtacgcgg tgcccggcct gcggcgcaag
     1141 gccgttctcg tggccctggc gctgcagcct ggacagatcg tccacgtgga ccggctgatc
     1201 gacatcgtct ggggcgatgc cgcgcccgcc accgcggcca acacgctgca ggcccacatc
     1261 tcccagctgc gccgcctgct cggcgggcgg cacacgatcc agtcccgccc cccggggtac
     1321 gtgctggacg tcggcgccga ggccaccgac ctggagcagg cggagcgtct gctccgcgag
     1381 gggacgcagg ctgccgaccc ggtcgacggt gtggcgctgg tgcgggccgc ggtctccctg
     1441 tggcgcgggg cgccgctggc cgacctcgcc gaactgccct ggttcggcga tcacgcacga
     1501 cggctggagc atctgctgct gcacgcccgg cagaccctcg tcgaggcccg tctggcgctg
     1561 ggcgagcaca ctgcgctggt accggagctg gaagccctca gccggcagca cccgctgcac
     1621 gagcagatcc accggcaact gatg
//
LOCUS       DASRSC010000181         7396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4321566,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000181 DASRSC010000000
VERSION     DASRSC010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4321566"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..143)
                     /locus_tag="VFE39_09030"
     CDS             complement(<1..143)
                     /locus_tag="VFE39_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET6258781.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKA"
     gene            complement(316..1119)
                     /locus_tag="VFE39_09035"
     CDS             complement(316..1119)
                     /locus_tag="VFE39_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HET6258782.1"
                     /translation="MARRTTVLEVRRTERLTPHMIRVVAGGDQLASFPDTSYTDRYVK
                     IIFPRRGVAYPEPFDMDEIRARLPREQWPATRTYTVRSFDPVGGELSIDFVHHGDAGI
                     AGPWAVAARPGDVLRLLGPGGAYAPDPDADWHLLVGDESALPAISAACERIPTGVPAR
                     AVLEVADAAEQQELSSPGDLRICWLHRDANAPDQLVEAVRALDFPPGRVHAFVHGEAG
                     LVRGVRTHLLAEREVARELLSVSGYWRLGRDEEGWREDKAVESAAAAGD"
     gene            complement(1211..1561)
                     /locus_tag="VFE39_09040"
     CDS             complement(1211..1561)
                     /locus_tag="VFE39_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HET6258783.1"
                     /translation="MAQIREIRLIDDLDGDAADETVEFGVDGKNYEIDLSKDNAEKLR
                     DALAAFVAAARRSGGGGGRRRGNSAASAPVRRPSIDREQNQAIRDWARKRGMKVSDRG
                     RIPAEVLDAYHQEN"
     gene            complement(1675..3129)
                     /gene="lysS"
                     /locus_tag="VFE39_09045"
     CDS             complement(1675..3129)
                     /gene="lysS"
                     /locus_tag="VFE39_09045"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET6258784.1"
                     /translation="MRVRRAKRAEMLAQGRDPYPVAVERTHSLREVRAAHGDLPPDTG
                     TGEKVGVTGRVIFLRNAGKLCFATLREGNGTELQAMLSLALVGEEELARWKSTVDLGD
                     HVFVRGEVATSRRGELSVMADRWEMAAKAVRPLPVAHRELSEETRVRQRYVDLIVRPQ
                     AREMVRNRAEVVRTLRSVLHERDFVEVETPMLQLQHGGATARPFVTHANALDTDLYLR
                     IAPELFLKRSVVGGIERVFEINRNFRNEGIDSTHSPEFAMLEAYQAYATYDDMADLTR
                     ELVLACARAVFGSTVVRHADGSEHDLGTPWRSVTLHDAVSKAVGQDVTLDTSVEDLRK
                     LAVAHDVELRDSLSAGEIVLELFEKLVEHTLVEPTFVRDYPIEVRPLTRQHRSEPRLA
                     EAWDLIVFGTELATAYSELVDPVEQRERLYAQSLLAAAGDVEAMQVDEDFLRAMEYGM
                     PPSGGLGMGIDRLLMTLTGLGIRETILYPIVRSE"
     gene            3260..3997
                     /locus_tag="VFE39_09050"
     CDS             3260..3997
                     /locus_tag="VFE39_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6258785.1"
                     /translation="MTASRARSFGAAAAEYARYRPEYAEAAIDRALEPVVARPTLRLL
                     DLAAGTGKLTGALLRRGSVIAVEPDPAMLAELRARFPESDARDGSAESIPVPAGSVDA
                     VLVGQAWHWFDAQRALPEIARVLRPGGVLAALWNGDDPDVEWVARYREAAGIASRLRA
                     DAGLRLPEHPDFGPSEHSAHPNPVRTTVESLIAMLATHSWSLVAEPAEREAAFARVRA
                     YLATRPETSAGEFELPLVTDVVRLLRR"
     gene            complement(3913..4767)
                     /locus_tag="VFE39_09055"
     CDS             complement(3913..4767)
                     /locus_tag="VFE39_09055"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HET6258786.1"
                     /translation="MLLCVDVGNTQIALGLYADTDTDDPEVHPPLVRDWRMRTDPRMT
                     SDELEVAVEALLGRYAAQVTGIAALSTVPSLLRELRLLIDRRSDPNVVVGPGVRTGVP
                     LLVDNPREVGADRVMNTLAAHRLFDTACVVVDFGTSTNIDVVSAKGEFLGGALAPGIE
                     VSLEALAARAAALRTVELVRPRSVIGKNTVECLQSGVLYGFAGQVDGLVTRILAELGP
                     KAGPVTVIGTGGLAPLMAGVSETITEFVPDLTLLGLRLTYLRNRRTTSVTSGSSNSPA
                     DVSGRVAR"
     gene            complement(4780..5655)
                     /gene="panC"
                     /locus_tag="VFE39_09060"
     CDS             complement(4780..5655)
                     /gene="panC"
                     /locus_tag="VFE39_09060"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HET6258787.1"
                     /translation="MTVRTQGGYAAGQLTVHRSPAEIAPVTRALRGAGRKVALVPTMG
                     ALHEGHRELIRHARRTPGATVTAVSIFVNPLQFGAGEDLDRYPRPLEADLQVCREEGV
                     ELVFLPGVTDMYPDGADTTIVPGRVGDELEGAVRPGHFAGVLTVVAKLFHIVAPEVAF
                     FGEKDYQQLVMIKKMVRDLDFPVSVVGVPTVREPGGLALSSRNAYLAPEERPVAATLQ
                     RALAAGAAVSARGPEAVLGAARAVLAEEPELQVQYLELRDPDLGRRPQTGRARLLVAA
                     RLGRTRLIDNIAVQL"
     gene            complement(5652..6545)
                     /locus_tag="VFE39_09065"
     CDS             complement(5652..6545)
                     /locus_tag="VFE39_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HET6258788.1"
                     /translation="MGADRPARLTVGVVSAGRVGAVVGAAWAAAGHHVVATSGVSRAS
                     VRRAAALLPGVPLRAPDEVVAAANLVLLAVPDDVLPGLVRGLAAAGSFRPGQIVVHTS
                     GAHGVGALRPAAEHGVLPLALHPVMTFTGRTEDVARLRGCSVGVTATADDEAAWSVGE
                     ALVVEMGAEPVRIPEEVRPLYHAALAHGANHLVTLVRDCVETLERAGVSPAERLVAPL
                     LSAALDNVLRHGDRALTGPVARGDVATVRTHLRELAAADPDLAETYRVLAARTARRAS
                     AAGLLSEYAAKDVLAILEGSP"
     gene            complement(6701..>7396)
                     /locus_tag="VFE39_09070"
     CDS             complement(6701..>7396)
                     /locus_tag="VFE39_09070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HET6258789.1"
                     /translation="LLLRGVLSPFAHPLFTVMTGIAAGIASRSRSIPVRVVTVLIGYL
                     LAVALHALWNGSASLFGGAVFFLVYGFIMVPMFLAVAYVVVWQRRREQRIVIDQLPGF
                     ARAGWIAPSEVPLLSSLAGRRGWRAAVRRRSGRQVAKAVSDYQDSVTELAFLRSRMER
                     GAVGASGAHWHDEALGGLLRARARAVGHPEALTVAMRHRGPQNWTPPPPGPPPSPAVP
                     PDAPPQSWPPPRR"
BASE COUNT         1121 a         2671 c         2527 g         1077 t
ORIGIN      
        1 gccttcgcgg ccactccctc gccctcgtgg atgaggccaa ggaggatgtg ctcggtgccg
       61 atgtagttgt ggttgagcat cctggcctct tcttgggcca gaacgacaac acgcctcgct
      121 cggtcggtga acctctcgaa catttacact cctagcctgg ctgcgccatg caccggcgcg
      181 cgctgcgccg tgtacacccc gggtaccact gtatcggccc gggggcggtg gtcgcctggg
      241 tcgttcgtag tggaaacaac gctgcaaccc gcgcggggat tccccgtcgg gccgttgcgc
      301 cggtgaactc gctgctcagt cgcctgcagc cgctgcactc tcgacggcct tgtcctcccg
      361 ccatccctct tcgtcgcggc ctagacgcca ataccccgag accgacagca gctcgcgcgc
      421 cacctcacgc tcggcgagga gatgagtccg gacaccgcgc accagtccgg cctccccgtg
      481 tacgaaggcg tgtacgcgcc cgggcgggaa gtccagggct cggacggctt cgacaagctg
      541 gtcgggcgca ttcgcatccc ggtggagcca gcagatccgc aggtcgcccg gtgagctcag
      601 ctcctgctgc tcggcggcgt cggccacctc caggacggcg cgcgccggca ctccggtcgg
      661 aatgcgctca cacgccgcgg aaatggcggg aagcgcgctc tcgtcgccca ccagcaggtg
      721 ccagtccgcg tcggggtccg gggcgtacgc gccgccgggg ccgagcagtc gcagcacatc
      781 gcccggacgg gcggccactg cccacggccc cgcgatgccc gcgtcgccgt ggtgcacgaa
      841 gtcgatcgac agttcgccac cgacggggtc gaaggacctg acggtgtagg tccgggtggc
      901 cggccactgc tcacgcggca gccgcgcccg gatctcgtcc atgtcgaacg gctcgggata
      961 agccactcca cgacggggga agatgatctt cacataccga tccgtgtagg acgtgtcggg
     1021 gaaggaggcg agctggtcgc caccagccac tactcggatc atgtgcgggg tcagccgctc
     1081 cgtgcggcga acctccagga cggtcgtcct gcgggccacg ggacctcctc taccggctcc
     1141 accggtgagg ccaccctaac aaatgtggaa cgggccggcg cacgccgccg gccctcaccc
     1201 gtcccgtcaa tcagttctcc tggtggtacg cgtccaatac ctcggccgga attcgaccgc
     1261 ggtcggacac cttcatgccc cgcttacggg cccagtcccg gatcgcctgg ttctgctcac
     1321 ggtcgataga cggacgtcgc accggggcgc tcgccgccga gttgccacgg cgacgaccgc
     1381 ctccgccgcc ggaacgccgg gcggctgcca cgaacgccgc gagtgcgtcc cgcagcttct
     1441 ccgcattgtc cttcgacagg tcgatctcgt agttcttgcc gtcgacgccg aactcaaccg
     1501 tctcgtcggc ggcatctccg tccaggtcgt cgatcagtcg gatctcacgg atctgcgcca
     1561 ccgtcgcagc cctccatact tcatcgttgt tgtctgcggc gacgccgcat agggcgcacg
     1621 atataactaa ttcgcggtga ctgtcgagtc gccctgcccc gcactcacgg tttgttactc
     1681 agaacgcacg atcgggtaca gaatggtctc cctgattccg agcccggtca gcgtcatgag
     1741 cagccggtcg atgcccattc ccagaccacc cgacggtggc attccgtact ccatcgcacg
     1801 gaggaagtcc tcgtcgacct gcatcgcctc gacatctccc gccgcggcca gcagggactg
     1861 cgcgtagagc ctttcccgct gctccacagg atccacgagt tcggagtagg cggtggcgag
     1921 ctccgtgccg aacacgatga ggtcccaggc ctcggcgagt cgcggctcgc tgcggtgctg
     1981 cctggtcagt ggccggacct cgatcgggta gtcccggacg aacgtcggct cgacgagtgt
     2041 gtgctcgacg agcttctcga acagttccag cacgatctcg ccggccgaca gcgaatcccg
     2101 cagttcgaca tcgtgagcca ctgcaagctt ccgcagatcc tccactgagg tgtccagcgt
     2161 cacgtcctgc ccgaccgcct tcgagaccgc gtcatggagc gtcaccgaac gccacggcgt
     2221 acccagatcg tgttcggacc cgtctgcatg ccggacgacc gtagaaccga agacggcgcg
     2281 ggcgcaggcc agcaccaact cccgcgtcag atcggccatg tcgtcgtacg tcgcgtaggc
     2341 ctggtaggcc tccagcatcg cgaactccgg cgagtgcgtc gagtcgatgc cctcgttccg
     2401 gaagttccga ttgatctcga agacgcgctc gataccaccc accacgctgc gcttgaggaa
     2461 aagttccgga gcgatccgca ggtacagatc ggtgtcgagt gcgttggcgt gggtgacaaa
     2521 cggacgcgcg gtggcaccgc cgtgctgcaa ctgcagcatc ggtgtctcca cctcgacgaa
     2581 gtcgcgctcg tggagtaccg accgcagtgt gcgcacgacc tcggcgcgat tgcggaccat
     2641 ctcgcgcgcc tggggtcgga cgatgaggtc gacgtagcgc tggcgcaccc gcgtctcctc
     2701 ggacagttcg cggtgcgcca ccggcagcgg gcgcacggcc ttcgcggcca tctcccagcg
     2761 gtcggccatc accgacagct cgccccgccg ggaggtggcc acctcgccgc gcacgaagac
     2821 gtggtcgccg aggtccacag tgctcttcca gcgggccagc tcctcctcgc ccaccagcgc
     2881 gagactcagc atcgcctgca gctccgtacc gttgccctcc cgcagagtgg cgaagcagag
     2941 cttgccggcg ttgcgcagga agatcacccg tccggtgact ccgaccttct cgccggtgcc
     3001 cgtatccggg ggcaggtcgc catgagcggc gcgcacctcg cggagactgt gcgtgcgctc
     3061 gaccgcgacg ggatagggat cccggccctg ggccagcatc tccgcccgct tggcccggcg
     3121 cacccgcatc tgctcgggaa gctcgttgtc ggggccgtcg aagggctcgg tgctcacgcc
     3181 tccaggttag tgacgtctgc atttgtgttt cccagccggt tgcccggcgg gagtccgggg
     3241 ctgacgtacg gtcgagtacg tgactgcttc ccgggctcga tccttcggcg ccgcggccgc
     3301 cgaatacgcc cgttatcgtc cggagtacgc cgaggccgcc atcgaccggg ccctggagcc
     3361 tgtggtcgca cgtcccacac tccggctgct ggacctggca gccggcaccg ggaaactcac
     3421 gggcgcgctg ctccgtcgcg gctcggtcat cgccgtcgag cccgacccgg cgatgctcgc
     3481 cgagctgcgg gcccggtttc cggagagcga cgcgcgcgac ggcagcgccg agtcgatccc
     3541 ggtgccggcc ggatcggtcg acgcggttct ggtgggccag gcgtggcact ggttcgacgc
     3601 gcagcgcgca ctgccggaga tcgctcgtgt gctacggccc ggcggggtgc tggctgccct
     3661 ctggaacggc gacgacccag acgtggagtg ggtggcccgc tatcgcgagg cggccggaat
     3721 cgcgtcgcgg ttgcgggccg acgcggggct ccggctgccc gagcaccctg acttcgggcc
     3781 gagcgagcac agcgcccatc ccaatccggt gcgcaccacc gtcgagagtc tgatcgcgat
     3841 gctcgccacg cactcgtggt cgctggtcgc cgaaccggcc gagcgcgagg ccgccttcgc
     3901 ccgggtccgg gcctacctcg ccacacgacc ggagacgtcg gcgggcgagt tcgagctgcc
     3961 gctcgtcacg gacgtggtgc gcctgttgcg cagataggtc agccgtagcc ccagcagcgt
     4021 caggtcgggc acgaactcgg tgatcgtctc ggacacgcct gccatcaggg gcgcgaggcc
     4081 accggtgccg atgacggtga ccgggcctgc cttcgggccg agctcggcga ggatgcgcgt
     4141 gaccaggccg tcaacctgcc cggcgaagcc gtagagcacg ccggactgca ggcactccac
     4201 cgtgttcttg ccgatcaccg agcggggccg aaccagctcg acggtgcgca gcgcggccgc
     4261 gcgggcggcc agtgcctcca gcgagacctc gatgccgggg gcgagggcgc ccccgagaaa
     4321 ctctcctttc gcggacacga cgtcgatgtt ggtggacgtc ccgaagtcga cgaccacgca
     4381 cgcggtgtcg aacagccgat gcgcggcaag cgtgttcatg acgcggtcgg cacccacctc
     4441 gcgcggatta tcgaccagca acggaacgcc tgtgcgcacc ccgggtccga cgacgacgtt
     4501 cggatcactg cggcggtcga tgagcagccg caactcccgg agcagactgg gcactgtgga
     4561 cagcgccgcg attccggtga cctgcgccgc gtagcgcccg agcaaagcct cgacggccac
     4621 ctcgagctcg tcggaggtca tgcgcggatc ggtgcgcatc cgccagtcgc gcaccagagg
     4681 cgggtggacc tcggggtcgt cggtgtcggt gtccgcgtaa aggccgagcg cgatctgggt
     4741 gttgccgacg tccacgcaga gcagcacggc gtgttcctcc tacagctgca cggcgatgtt
     4801 gtcgatcagc cgggtccggc cgagccgggc cgcgaccagc agccgcgccc gaccggtttg
     4861 ggggcgtcgt ccgaggtcgg ggtcgcggag ctcgaggtac tgcacctgca gctccggctc
     4921 ctcggccagc accgcgcgcg cggcgcccag taccgcttcc ggcccgcggg ccgacaccgc
     4981 agccccggcg gccagcgcac gctggagcgt ggcggccacg gggcgctcct cgggtgcgag
     5041 gtaggcgttg cggctcgaga gggccaagcc gccgggctcg cggaccgtcg gcaccccgac
     5101 caccgatacc gggaagtcca ggtcgcgcac catcttcttg atcatcacca gctgctggta
     5161 gtccttctca ccgaagaacg cgacctctgg cgcgacgatg tggaagagct tcgcgacgac
     5221 ggtgagcaca ccggcgaagt gaccgggccg tacggcgccc tcgagctcgt cgccgacccg
     5281 ccctgggacg atcgtggtgt cggcgccgtc ggggtacatg tccgtgacac cggggaggaa
     5341 caccagctcg acgccctcct cgcggcacac ctgcagatcg gcctcgagcg gcctgggata
     5401 gcggtcgaga tcctcgcccg ccccgaactg cagcgggttc acgaagatcg acacagcggt
     5461 gactgtggcg cccggcgtcc gacgggcgtg tcggatcagc tcccggtggc cctcgtgcag
     5521 agcgcccatc gtcggcacga gcgccacctt gcggcccgcg ccgcgcagcg cacgcgtcac
     5581 cggcgcgatc tcggcgggcg accggtgcac ggtcagctgt ccggcggcgt atccgccctg
     5641 cgttcggacc gtcacggtga accttccagg atggcgagca cgtccttcgc ggcgtactcg
     5701 gacagcaggc ccgctgcgga cgcccggcgt gccgtccggg cggcgagcac gcggtaggtc
     5761 tcggcgaggt cagggtcggc ggcagcgagc tcgcgcaggt gtgtgcgcac cgtcgcgacg
     5821 tcgccccgcg ccaccgggcc ggtcagcgcc cggtcaccgt ggcgcagcac gttgtccagc
     5881 gcggccgaca gcagcggtgc gacgagccgt tcggccgggc tgacgccggc gcgctccagg
     5941 gtctcgacgc agtcgcggac aagcgtgacg aggtggttcg ccccgtgtgc gagcgcagcg
     6001 tggtacagcg gtcggacctc ctccgggatc cgcacgggct ccgcacccat ctccacgacc
     6061 agagcctcgc cgacgctcca cgcggcctcg tcgtctgccg tggctgtcac accgacgctg
     6121 cagccccgga gccgcgcgac gtcctcggtg cgaccggtga acgtcatcac ggggtgcaga
     6181 gcgagcggca gcacaccgtg ctcggcggca ggacggagag caccgacacc gtgggcaccg
     6241 gaggtgtgca cgacgatctg gcctggacgg aagctgccgg cggcagccag ccctcggacc
     6301 aggccgggca gcacgtcgtc cggaactgcg aggagcacga ggtttgcggc cgcgacgacc
     6361 tcgtcgggtg cccgcagcgg cacgccgggc agcagtgcgg cagcacgccg caccgacgcc
     6421 cgggacaccc cggaggtggc gaccacgtgg tgcccggcgg cggcccaggc cgcccccacg
     6481 acggccccga cgcgaccagc cgagacgacc ccgacggtga gccgggccgg cctgtctgca
     6541 cccatcactt ctcctcgttc gttcggcgtc cccgctcggg taccggacga tgatgagtcg
     6601 ccgcgagcac aggttacgcg gctacgcagc accgacgagg acagctgggc ggcttcacca
     6661 gaacagtggc ccaacgggtg tgacgcactc gccgcgccac tcagcggcga ggtgggggcc
     6721 agctctgtgg cggcgcatcg gggggcaccg cggggctcgg cggtggtccg ggcggtggcg
     6781 gcgtccagtt ctgcggaccc cggtgccgca tcgccaccgt cagggcctcc gggtgcccca
     6841 ccgcacgcgc gcgcgcccgc aggagcccgc cgagcgcctc gtcgtgccag tgcgcccccg
     6901 acgcaccgac agcaccgcgc tccatcctcg agcggaggaa cgcgagctcg gtgacgctgt
     6961 cctggtagtc cgagacggcc ttcgccacct gcctacccga tctacgccgc acggccgcac
     7021 gccaccctcg ccgtccggcc aggctggaca gcagcggcac ctcgctcggc gcaatccacc
     7081 cggcccgcgc gaaaccgggc agctgatcga tcacgatgcg ctgctctcgc cggcgctgcc
     7141 agacgacgac atatgccaca gccaggaaca tcggcaccat gatgaagccg tagaccagga
     7201 agaagacggc gccgccgaaa agcgatgccg acccgttcca cagggcgtgc agcgccaccg
     7261 ccaggagata gccgatgagc accgtgacca cgcggacggg gatgctgcgg ctccgcgaag
     7321 cgatgccggc ggcgataccg gtcatcacgg tgaacagcgg atgggcgaac ggtgagagca
     7381 cgccccgcag caggag
//
LOCUS       DASRSC010000182        11102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_882901,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000182 DASRSC010000000
VERSION     DASRSC010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11102
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882901"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..604)
                     /gene="phnH"
                     /locus_tag="VFE39_09075"
     CDS             complement(17..604)
                     /gene="phnH"
                     /locus_tag="VFE39_09075"
                     /inference="COORDINATES: protein motif:HMM:NF017645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HET6258790.1"
                     /translation="MTGAAVAPPLVRPGNRDAHRAFRAVLDALARPGTTTHLPVSAGV
                     LSALGPLLALADLDTPVCVLGDGAVATEQWLGAVALATSAPASGLGEARLVAVLRPVT
                     VDELRAVRVGSPADPEEAALVTLAVPALTGGPTVRLTGPGTAAGAVITATGLPAGWLE
                     AREAVAFPAGADLLLVDPDGACVGLPRSTRTTGGS"
     gene            complement(601..1056)
                     /locus_tag="VFE39_09080"
     CDS             complement(601..1056)
                     /locus_tag="VFE39_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HET6258791.1"
                     /translation="MSVTEQRVAAAESREERVGLLAQAGTAELVALADGCLADGARLA
                     VLVPPEVGCVATEVLEPVGHERFLLGDVLACRAEVELGGSRGWAMRLGDDRAAVLAAA
                     VCDAEAEADRPMAGEVAALCHRVAQRVAAAEAAEWAALAPTVVAFEELA"
     gene            complement(1062..1799)
                     /locus_tag="VFE39_09085"
     CDS             complement(1062..1799)
                     /locus_tag="VFE39_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6258792.1"
                     /translation="MGSVIPSRGTRYLDIAEQLATELPDLPAGARVAGEHDITLRFGV
                     SRAAARAALQELERRLLVRRIQGAGTFVNRRIDYVISHGRRPSWHETVAAAGAHPRTV
                     VRGVDVVALPEGPAGLLDLPPGTRAHRLRRQSYIDDLLAAASHEWIPVATVPELGAAL
                     HAAESVDLVLRQVGRVQPVRSWSRVSLDVPPADVLAGLQMESTAPVWLVESLSRDGEG
                     GPPVMCSSSWMRADALRVVVELGEGPG"
     gene            2110..3066
                     /locus_tag="VFE39_09090"
     CDS             2110..3066
                     /locus_tag="VFE39_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HET6258793.1"
                     /translation="MNRTLRHVLPAVAAVLLTVALAACGESASTATGGGGTGAADPDT
                     LVFAAVPSEQSTSLQADFKPVLDMLARETGKKIEFRQATDYAAVIEGQLSGQVQIAQY
                     GPLSFVLAQAKGAAITPVGAQISAKGATPGYQSYAITKAGSPIKSIADFAGKKICFVD
                     PNSTSGYLYPTAALLQAGIDPTTGIQPIFAGGHDASVLEVASGRCDAGFAEDSMVDTT
                     LIQKGQLKPGDITTLWKSDIIPGSPVAISTSLSPELQAKLTDAFQNKANADYLKANGF
                     CDGTCTVGDGGYWGYAPVDNAFYDPIRKVCDTTKNKNCTTAS"
     gene            3063..3890
                     /gene="phnC"
                     /locus_tag="VFE39_09095"
     CDS             3063..3890
                     /gene="phnC"
                     /locus_tag="VFE39_09095"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HET6258794.1"
                     /translation="MTGRGRTDGSANGAGTPPVVEFAGVTKRFGPDVLGLDDVSLTVH
                     TGEVVVLLGLSGSGKSTLLRHVDGLQLPTSGTVRVLGVDVGAARGSELRALRRRIGFV
                     FQQFDLVGSRTVLENVCTGALGRVRGPRLGLPTYPRALRREALEHLDRVGLGDRAFQR
                     ADTLSGGQQQRVAVARALMQRPEVLLADEPVASLDPESSEQVMALLKTISRENSLTVL
                     CSLHQVDLALGWGDRIVGLRTGRTVLDLPTAGLGRDDVMAVYSGVGADAGALLTTAG"
     gene            3890..5647
                     /gene="phnE"
                     /locus_tag="VFE39_09100"
     CDS             3890..5647
                     /gene="phnE"
                     /locus_tag="VFE39_09100"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019815637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HET6258795.1"
                     /translation="MAAPTAGAGAPQRLAARPSASSTVAVFVLLAILAAALWAFADLR
                     INVATFVDGSHQAVAFARRTLPLDFPPLPEILRLCGQTLAIVILATLLSTILSVPLAL
                     LAATTTTPNDASRLGARALIVVARAVPDVVLAIVFVRIFGIGALTGVLAMGLHSVGMI
                     GKMYADAIEQIDAGAPTALTATGATRLQRIAGGVLPPVLPAFVAIGLHRLDINLRVSV
                     VLGFVGVQGIGFAIADSLAVLDYQRGIALALVVLVLCIVVELISGAIRTALLGRAAAG
                     PLRGISGGLSRLVHVRSAIGRHTERRDDGLDLDADLTRDPRLRSAAALVEGRLAPPWD
                     ARRISAATYTAITAAVLVASVVASGVSPLEFLEALPRVLASLGLFLPPETAGLFGALL
                     EQLLVTVQIALAATLIGVVLSLPIGALAARNVAPTPGVANAFRLVILLIRGIPELILA
                     IVFVVITGLGQVAGAVALGVGAVGFLGKLVADSLEEVDAGPQRALLATGGSRSQVFFA
                     ATVPAGARAIVAHVLYLLDTNIRSATLLGIVGAGGIGFYLISAARVLEFGVVTTILIM
                     IFCTVLLVELLAIWLRDRF"
     gene            5677..6195
                     /locus_tag="VFE39_09105"
     CDS             5677..6195
                     /locus_tag="VFE39_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET6258796.1"
                     /translation="MNIRETVRTDLLAMADLPYDGEEVDQLAHALQAGTLAAAADGRP
                     ALVAAALLHDVGRLPHVEAALPAAPHERAGAAYVTALLGAEVGWLVGSHVLAKRALVA
                     TDPDYFALLSPTSVRTLAEQGGPAAGDEVERFLHHRLAADAMQLRRWDDQAKDRAARP
                     MPIDDLLDAALG"
     gene            complement(6208..6522)
                     /locus_tag="VFE39_09110"
     CDS             complement(6208..6522)
                     /locus_tag="VFE39_09110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258797.1"
                     /translation="MIRRAATLVLTALIAFVLAGCGGGGSQTDCGLDGCTITFPRSGA
                     AAVSVLGIEARVVGVEGGTAELEIAGQRITVPVGGETQAQGFTVGVERVTDTEVVVRI
                     RP"
     gene            6776..7975
                     /locus_tag="VFE39_09115"
     CDS             6776..7975
                     /locus_tag="VFE39_09115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6258798.1"
                     /translation="MTGPTVPTAERTADRRAEFIDAADRLAERFAERAAAHDQDGSFP
                     HENYVDLADAGLLRLSVPQELGGLGAGLGDVIAVLERLAAGDGATALSVTMHISPLGQ
                     WGAVWRRTGSLRLAHLLREAAAGSLVWAAITGEPGVRNDMTDARTVAEKVAGGYLLTG
                     RKIFATNTAIATHFSTTARHDGADDGPRLLLCQVAMDQPGVRVHPTWNAMGMRATRSD
                     DLELDGVFVPDEQVVHSLPVGHLDRRVLETVWAWAMPAFGAVYTGIAAGALDWTVRRV
                     IRSERAEDGVVQDVIGACRILLETSRAMLYRHVDEVENGRLLDQGVQSGLARCAMVKY
                     VAANNAVEVLHRLVDVVGGPAYTRNLPFERMWRDVQAGPIMPMGNLAARRLVGADTLG
                     VVTAPVS"
     gene            complement(8003..8773)
                     /locus_tag="VFE39_09120"
     CDS             complement(8003..8773)
                     /locus_tag="VFE39_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009078026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET6258799.1"
                     /translation="MTAVRVERSGPVTTVFLHRPARRNAVDGATAAALADAFRTFDAD
                     EDAAVAVLHGEGGVFCAGADLTAVGTERGNHVAPDGDGPMGPTRMRLSKPVIAAVSGH
                     AVAGGLELALWCDLRVADETAVFGVFCRRWGVPLIDGGTVRLPRLIGTSRAMDLILTG
                     RPVDAAEALAIGLANRVVPPGEALAAAQELAAQLAAFPQTCMRRDRLSMLDQDGLSEA
                     DALDAEFAHGQVSLAADALAGAARFAGGAGRHGSFGDS"
     gene            complement(8770..9261)
                     /locus_tag="VFE39_09125"
     CDS             complement(8770..9261)
                     /locus_tag="VFE39_09125"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258800.1"
                     /translation="MLAVELVRRGAARHAGSTAVVAGAPELTAETCTVDGWVRTRDLA
                     RIDERGYVTLVDRTSDMIITGGYNVYPREVEDVLLAHPAVAAAAVVAAPDETWVEAVT
                     AFVTLWPGVPPPAADELQGAVRAQLAGYKVPKQVHVVDALPLSPVGKVLRRALRDPLW
                     GRS"
     gene            9664..10419
                     /locus_tag="VFE39_09130"
     CDS             9664..10419
                     /locus_tag="VFE39_09130"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6258801.1"
                     /translation="MADSGIDSVLRDRVALITGASGGIGQEIARRLAESGTDLLLTYG
                     GHADEAEHVADQARKLGRRAEVMQADFADPDTPARVVAAADERLGGIDVLVPAAGVAA
                     QKSWEDVDGALWDETMTVNVRAPFFLAQQTLPRMVEQRFGRILLFSSVAAFTGGVIGP
                     HYAAAKAALHGLVHNLSANVAGSGVTINAIAPALIAGTRMLPLGPDDPVPARIPVGRL
                     GTTEEVADLSLAMLRNGYLTSKVIALDGGAYPD"
     gene            complement(10385..>11102)
                     /locus_tag="VFE39_09135"
     CDS             complement(10385..>11102)
                     /locus_tag="VFE39_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HET6258802.1"
                     /translation="FLWILGILNLVVLIGIIRIFRRMRTGEYDEQELEEQLNKRGFMN
                     RFLNGLTRSITKPWHIYPVGVLFGLGFDTATEVGLLVLAGGAAAFSLPFYAILVLPIL
                     FAAGMSLLDSLDGLFMNFAYGWAFARPVRKVFYNITITALSVTVALVIGTIELVSVIT
                     QQAGIDSGPLAAVASIPLNDAGYGLVALFAIAWLVAFAIWRLGHVEQRWSATPGEPGT
                     ATGLTGGRCLSPGTPRRRER"
BASE COUNT         1549 a         4100 c         3913 g         1540 t
ORIGIN      
        1 cccgagtacc ccattgtcag ctccctccag tcgtgcgcgt gctgcgcggc aggccgacgc
       61 aggcaccatc cgggtcgacg agcagcaggt cggcgcctgc ggggaacgcc accgcttccc
      121 gggcctcaag ccagcccgcc ggcagcccgg tcgcggtgat cacggcgccg gccgctgtgc
      181 cgggcccggt caggcgcacc gtcgggccgc cggtcagggc gggcacggcg agggtgacca
      241 gcgcggcctc ctccgggtcg gccggactcc ccacgcggac ggcccgcagc tcgtccaccg
      301 tgaccgggcg cagcaccgcg accagccgcg cctccccgag gcctgacgca ggcgccgacg
      361 tcgcaagagc gacggcgccg agccactgct cggtggcaac ggcgccgtcg ccgagcacgc
      421 acaccggcgt gtcgaggtcg gcgagggcga gcagcggtcc gagcgcggac agcacccccg
      481 ccgataccgg caggtgggtg gtcgtccccg gccgggccag tgcatcgagg acggcgcgga
      541 aggcgcggtg ggcgtcccgg ttgccgggcc ggaccagcgg cggcgcaact gcggcccccg
      601 tcacgcgagc tcctcgaacg cgacgacggt gggggcgagg gctgcccact cggcagcttc
      661 ggccgcggcc acgcgctgcg cgacgcggtg gcacagggca gccacctctc ccgccatcgg
      721 cctgtccgcc tcagcctcgg cgtcgcacac ggctgcggcg agcacggccg cccggtcgtc
      781 accgaggcgc atcgcccacc cccggctccc gcccagctcc acctcggcac ggcacgcgag
      841 cacgtcgccc agcaggaacc gctcgtggcc gaccggctcc agcacctccg tcgccacgca
      901 cccgacctcc ggcgggacca acaccgccag ccgcgcgcca tcggccaggc acccgtcggc
      961 cagcgccacg agctcggcgg tcccggcctg cgccagcaac ccgacccgct cctcccgcga
     1021 ctccgcagcc gctacccgtt gctctgtcac gctcataccg ctcatcccgg tccttccccg
     1081 agctccacca ccacgcggag ggcatccgcg cgcatccaag aactgctgca catcactggg
     1141 ggcccgccct cgccgtcacg gctgaggctc tccaccagcc agaccggtgc cgtcgactcc
     1201 atctgcagcc cggcgagcac gtccgcgggc ggcacgtcga ggctcacgcg actccacgac
     1261 cgcaccggct gaacgcgtcc gacctggcgc agcaccaggt cgacggactc ggcggcgtgc
     1321 agcgcggcgc ccagctccgg gacggtcgcg accgggatcc actcgtgcga cgcggccgcc
     1381 agcaggtcgt cgatgtagga ctggcggcgt agccggtgcg cgcgggtgcc cggcggcagg
     1441 tcgagcaggc cagcggggcc ctccggcagg gccaccacgt cgacgccgcg cacgaccgtc
     1501 cggggatgtg ctcccgccgc ggccacggtc tcgtgccacg acgggcgccg gccgtgggag
     1561 atgacgtagt cgatccgccg gttgacgaac gtcccggcgc cctggatccg gcgcaccagg
     1621 agcctgcgtt ccagctcctg caaggccgcg cgcgccgccg cccggctgac gccgaaccgc
     1681 agggtgatgt cgtgctcccc ggcgacgcgc gcccccgcgg gcagatcggg cagctcggtc
     1741 gcgagctgct cggcgatgtc caggtaccgc gtgcctcggg aagggatgac ggagcccatg
     1801 tccacgaccc ttcggcagac gatgttcgcg agaacgaggc tgcccctccg acttgtccgg
     1861 acaactccgc tgacacgaac ggggggcggt ccccgggtga accccggtga cggttgtcgc
     1921 cgaccaagtg gggcaaaggg gatcagtcgc gagttgagcg ggcatgggca gcgaaccgtt
     1981 actgcgcgtt acgcctgtcg taacccgggg gaccgcccgc cagcatcgcg ggcgcggagg
     2041 ctcggccctg ttgtcggacc gccggaccgt cagtcccgtc tcgcgccgaa cagggagacc
     2101 agacctgcca tgaaccgcac tctccgccac gtgcttcccg ccgtggctgc cgtgctcctc
     2161 accgtcgccc tcgctgcgtg cggcgagagc gcctccaccg ctaccggcgg cggtggaacg
     2221 ggagcggccg acccggacac cctggtcttc gccgccgtcc ccagcgagca gtcgacgagc
     2281 ctgcaggcgg acttcaagcc ggtcctcgac atgctcgcgc gcgagaccgg aaagaagatc
     2341 gagttccgcc aggcgacgga ctacgccgcg gtgatcgagg gccagctctc cgggcaggtc
     2401 cagatcgccc agtacggccc gctgtcattc gtgctcgcgc aggccaaggg cgccgcgatc
     2461 accccggtcg gggctcagat cagcgccaag ggcgccaccc ccggctacca gtcctacgcc
     2521 atcacgaaag cgggttcgcc gatcaagtcg atcgcggact tcgcgggcaa gaagatctgc
     2581 ttcgtcgacc ccaactcgac gtccgggtat ctctacccca ccgcagctct gctacaggcc
     2641 gggatcgacc cgaccaccgg tatccaaccg atcttcgccg gcggccacga cgcctccgtt
     2701 ctggaggtcg cgtccggtcg ctgcgatgcc gggttcgccg aggactcgat ggtggacacg
     2761 acgctcatcc agaaggggca gctgaagccc ggcgatatca ccaccctctg gaagtccgac
     2821 atcattcccg gctcgcccgt cgcgatcagc acgtcgctct cccccgagct gcaggcgaag
     2881 ctcaccgacg cctttcagaa caaggcgaac gccgactacc tgaaggccaa cggtttctgc
     2941 gacgggacct gcacggtcgg cgacggcggc tattggggtt atgcgccggt ggacaacgcc
     3001 ttctacgacc cgatccgcaa ggtctgcgac accaccaaga acaagaactg caccaccgca
     3061 tcatgacggg cagggggagg acggacggga gcgcgaacgg cgccggcacc ccgccggtgg
     3121 tggagttcgc cggggtcacg aagcggttcg gtcccgacgt cctcggcctc gacgacgtct
     3181 ccctgacggt gcacacgggc gaggtggtcg tcctgctggg tctgtccggt tcggggaagt
     3241 ccactctcct gcgccacgtc gacggcctgc agctgcccac ctcgggcacg gtgcgggtgc
     3301 tcggggtcga cgtcggcgcg gctcgcggca gcgagctgcg ggcgctgcgc cgccggatcg
     3361 gcttcgtctt ccagcagttc gacctggtgg gcagccgcac ggtgctggag aacgtgtgca
     3421 caggcgccct cggccgggtc cgcgggcccc gcctgggcct gccgacgtac ccgagagcgc
     3481 tgcgccgtga ggcgttggag cacctggaca gggtggggct gggcgaccgg gcgttccagc
     3541 gggcggacac cctgtctggc gggcagcagc agcgggtcgc ggtggcccgc gcgctgatgc
     3601 agcggcccga ggtgctgctg gccgacgagc cggtggcatc gctggacccc gaatcgtccg
     3661 agcaggtgat ggccctgctg aagacgatca gccgggagaa ctcgctcacc gtgctctgca
     3721 gcctgcacca ggtggacctc gcgctcggct ggggcgaccg gatcgtgggc ctgcgcaccg
     3781 ggcggaccgt cctcgacctg ccgaccgccg gactgggccg cgacgacgtg atggcggtgt
     3841 actccggcgt cggcgccgac gcgggagcgc tgctgacgac cgcgggttga tggccgcccc
     3901 caccgccggc gccggggcgc cccagcggct cgcggcgcga ccgtcggcgt cgtcgacggt
     3961 cgccgtgttc gtgctgctcg cgatcctggc tgcagcgctg tgggcgttcg ccgacctgcg
     4021 gatcaacgtc gcgacgttcg tcgacggctc ccaccaggcc gtggcgttcg cccggcggac
     4081 gctgccgctc gacttcccgc cgttgccgga gatcctccgc ctctgcgggc agaccctcgc
     4141 catcgtcatt ctggccaccc tgctctcgac gatcctgtcg gtgccgctgg cgctgctcgc
     4201 tgccaccacc acgaccccga acgacgcgag tcgcctcggg gcgagggcgt tgatcgtcgt
     4261 cgcccgcgcg gtgcccgacg tcgtgctggc gatcgtcttc gtccgaatct tcggcatcgg
     4321 cgcgctcacc ggcgtactgg ccatgggcct gcactccgtc ggcatgatcg gcaagatgta
     4381 cgccgacgcg atcgagcaga tcgacgccgg agcacccacc gcgctcacgg cgaccggcgc
     4441 cacccgcctg cagcggatcg cgggcggggt cctccccccc gtgttgccgg cgttcgtcgc
     4501 gatcgggctg caccggctcg acatcaacct gcgcgtctcc gtcgtgctcg ggttcgtggg
     4561 cgtgcaggga atcggcttcg ccatcgcgga ctcgctggcc gtgctcgact accagcgcgg
     4621 gatcgcgctg gcgctggtcg tgctcgtgct gtgcatcgtc gtcgagctga tctcaggggc
     4681 catccgcacc gcgctcctgg gccgcgcggc agccggcccc ctgcgcggga tatcgggcgg
     4741 cctgtcccgg ctcgtccacg tccggagtgc gatcggtcgg cacacagagc gccgggacga
     4801 cgggctagac ctggacgcgg acctcactcg ggatccgcgc ctgcggtccg ccgcggccct
     4861 cgtcgagggc aggctggccc cgccgtggga cgccaggcgg atctctgcgg cgacctatac
     4921 ggcaatcaca gctgccgtgc tcgtcgcgtc cgtcgtcgcc tcgggcgtct cacccctgga
     4981 gttcctcgag gcgctcccgc gggtgctcgc cagcctgggg ctgttcctcc cacccgagac
     5041 ggccggcctc ttcggggcgc tgctcgaaca gctgctggtg accgtgcaga tcgcgctcgc
     5101 cgcgacgctg atcggcgtgg tgctgtccct cccgatcggt gcgctcgccg cgcgcaacgt
     5161 cgcgcccacg ccgggcgtcg cgaatgcgtt ccggctcgtc atcctcctca tccgcggcat
     5221 cccggaactg atcctcgcga tcgtcttcgt ggtgatcacg ggcctcggcc aggtggcagg
     5281 tgcggtggcg ctcggggtgg gcgcggtggg cttcctcggc aagctggtgg cggactcgct
     5341 ggaggaggtc gacgccgggc cacaacgggc cctgctcgct accggcggtt cacggtcaca
     5401 ggtgttcttc gcggcgacgg tcccggccgg ggcccgcgcg atcgtggcgc acgtgctcta
     5461 cctgctcgac acgaatatcc gctcggcaac gctgctcggc atcgtgggtg cgggcgggat
     5521 cggcttctac ctgatcagcg ccgcacgggt gctcgagttc ggcgtcgtga ccacgatctt
     5581 gatcatgatc ttctgcacgg ttctgctcgt cgaactgctg gcgatctggc tgcgggaccg
     5641 gttctgagtg gcttctccga cagagatgac ggttccatga acatccgcga aacggtgcgc
     5701 acggacctgc tggccatggc cgacctgccc tacgacggcg aggaggtcga tcaactcgcc
     5761 catgctctgc aggccggcac cctcgccgcc gcggcggatg gccggcccgc gctggtggcc
     5821 gcggccctgc tgcatgacgt gggcaggctg ccgcacgtgg aggccgcgtt gcctgcggct
     5881 ccgcacgagc gggcgggcgc cgcgtacgtc accgcgttgc tcggtgcgga ggtggggtgg
     5941 ctggtcgggt cgcacgtcct ggccaagcgg gcgctggtgg ccaccgaccc cgactacttc
     6001 gccctgctct ccccgacctc ggtgcgcacc ctcgccgaac agggcgggcc ggccgccggg
     6061 gacgaggtgg agcgattcct gcaccatcgg ctcgccgccg acgcgatgca gctgcggcgg
     6121 tgggacgacc aggccaagga ccgggccgcc cggccgatgc cgatcgacga cctgctggac
     6181 gcagccctcg gctgaccggc ggcaggctca gggccggatc cgcaccacga cctcggtgtc
     6241 ggtgacccgc tccaccccca ccgtgaagcc ctgggcctgc gtctccccgc ccaccggaac
     6301 ggtgatgcgc tgcccggcga tctccagttc ggccgtgccc ccctccacgc cgacgacccg
     6361 ggcctcgatg cccagcacgg acaccgccgc ggcaccgctg cgcgggaacg tgatggtgca
     6421 gccgtcgagc ccgcagtcgg tctgcgatcc tccgccccca cacccggcga ggacgaacgc
     6481 gatgagagcg gtcaggacga gcgtggcggc acgacggatc atgatcagat cgtacgggcc
     6541 gggcgcccga tttgtccggt acccgggtgg ccgcacacgc ggggcgacgg ggcgtgctcg
     6601 cggaggtggc gcaggggcca cggtggcccc ctgggcgagg cggcgcaccc gcccgaggat
     6661 aggggcttcc acctcgggac gctgctggtc gaggccatcc ttccggttga cgtcagggcg
     6721 gccggtcaac cgtacggtgg gcagccaggc gaccacacgg aatcccgggg gacccatgac
     6781 cggaccgaca gtgccgacgg cggagcgcac ggccgatcga cgcgcggagt tcatcgatgc
     6841 ggcggaccgg ctcgccgagc gcttcgccga gcgtgcggca gcgcacgacc aggacggcag
     6901 cttcccgcac gagaactatg ttgacctcgc cgatgccggt ctgctgcgcc tctccgttcc
     6961 gcaggagctc ggcggcctgg gagcggggct cggcgacgtc atagcggtgc tcgaacggct
     7021 cgcggcgggc gacggcgcca cggcgctctc cgtcacgatg cacatctcgc cactgggcca
     7081 gtggggcgcc gtgtggcggc gcaccggtag cctccggctg gcgcacctgc tgcgcgaggc
     7141 ggcggccggg tcgctggtct gggcggcgat caccggtgag cccggcgtcc gcaacgacat
     7201 gacggatgca cggacggtgg cggagaaggt cgccggcggg tacctcctca ccggccggaa
     7261 gatcttcgca accaacaccg cgatcgccac ccacttctcg acgacggccc ggcacgacgg
     7321 cgccgacgat gggccacggc tgctgctgtg ccaggtggcg atggaccagc ccggtgtgcg
     7381 ggtgcacccc acgtggaacg cgatggggat gcgggccacc cgtagcgacg acctggagct
     7441 cgacggcgtc ttcgtgcccg acgaacaggt cgtgcactcg ctcccggtgg ggcatctgga
     7501 tcggcgggtg ctggagacgg tgtgggcgtg ggcgatgccg gcgttcgggg cggtctacac
     7561 gggcatcgcc gcgggagccc tcgactggac cgtgcgccgg gtcatccgct ccgagcgcgc
     7621 ggaggacggc gtcgtgcagg acgtcatcgg cgcctgccgg atcctgctgg agacctcgcg
     7681 ggcgatgctg taccgccacg tcgacgaggt ggagaacggt cgtctcctgg accagggcgt
     7741 gcagtccggg ttggcccggt gcgcgatggt caagtacgtg gccgcaaata atgccgtcga
     7801 ggtgctgcac cggctcgtcg acgtcgtggg cggccccgcc tacacccgga acctgccgtt
     7861 cgagcggatg tggcgtgacg tccaggccgg cccgatcatg ccgatgggca acctcgcggc
     7921 ccggcgcctt gtcggcgccg acacgctcgg cgtggtgacg gcaccggtct cctgaccgga
     7981 tcggacgctg ctcacgggcg gatcaggaat cgccgaacga cccgtgcctg cccgctcccc
     8041 cggcgaaccg cgccgcgccg gcgagcgcat cggcggccag cgagacctgg ccgtgtgcga
     8101 actcagcatc cagcgcatcg gcctcggaca gcccgtcctg gtcgagcatc gagaggcgat
     8161 cacgccgcat gcaggtctgc gggaacgccg ccaactgcgc cgccagctcc tgggccgcgg
     8221 cgagggcctc ccccggtggg acgacgcggt tggcaagccc gatcgccagc gcttccgcgg
     8281 cgtcgaccgg acgcccggtg aggatcaggt ccatcgcccg gctggtgccg atcagccggg
     8341 gcaaccgcac ggtgccgccg tcgatcagcg ggacgcccca gcgccggcag aagacgccga
     8401 acaccgccgt ctcgtcggcc acgcgcaggt cgcaccacag ggccagctcc aacccgcccg
     8461 ccacggcgtg cccggacacc gcggcgatca ccggcttcga cagccgcatc cgggtcggcc
     8521 ccatcggccc gtcaccgtcg ggggcgacgt ggttgccgcg ctcggtgccg accgccgtca
     8581 gatcggcgcc cgcgcagaag acgccgccct cgccgtgcag cacggcgacg gcggcgtcct
     8641 cgtcggcgtc gaaggtgcgg aacgcgtcgg ccaacgcggc ggccgtcgcc ccgtcgacgg
     8701 cgttgcggcg ggcgggccgg tgcaggaaca ccgtggtgac ggggccgctc cgctccacgc
     8761 ggacggcagt cacgaccggc cccacagtgg gtcgcgcagc gcccggcgca gtaccttgcc
     8821 caccggggac aacgggagcg cgtccacgac gtgcacctgc ttcggcacct tgtacccggc
     8881 gagctgggcg cggaccgccc cctgtagctc gtccgcagcc ggcggcggca caccgggcca
     8941 gagcgtgacg aacgcggtga ccgcctccac ccacgtctcg tccggcgccg cgaccacggc
     9001 ggccgcggcg acggccgggt gggccagcag cacgtcctcg acctcccggg ggtagacgtt
     9061 gtaccccccg gtgatgatca tgtcgctggt gcggtcgacc agggtcacgt agccgcgctc
     9121 gtcgatgcgg gcgaggtcgc gggtgcgcac ccacccgtcg acggtgcacg tctccgcggt
     9181 cagctccggt gcgccggcca cgaccgccgt gctgcccgcg tgccgggcgg cccccctgcg
     9241 caccaactcg acagccagca tccgcacccc ccagtcggtc gcacgttcaa cctatctccg
     9301 gatccgcggc cccggacgtc acggcgcgcg ggcgcctgct gagccgtgac aactgccaag
     9361 attttctcct ggggttggta acggttcgcc gtcccgatcc gagcgtttag gaaccgctcg
     9421 gcgccaatcc ccccgggagt cgggcggcaa gctgcggtcc cgctggacgc cgtgttcagc
     9481 ggggccgcag cgtccatccg gcacccaggt tggtggcggg gtccagcgtt ccagccacac
     9541 ccaccacacg gccctgcgtc atcaactcgg gacagtggtg atccttgagt agtgccacga
     9601 gcaggcccgc ccggtcgtac tgtcggaatc gatgtgccga cagtctgatg gaggaggatg
     9661 cacgtggccg acagtggcat tgactcggtt ctgcgcgatc gcgtggccct gatcaccggg
     9721 gcctccggtg gcatcggcca ggagatcgcc cggcgcctcg ccgagagcgg caccgatctg
     9781 ctgctcacct acggcgggca cgccgatgag gccgagcacg tggcggacca ggcgcgcaag
     9841 ctcggccgcc gcgccgaggt gatgcaggcc gacttcgcgg accccgacac tccggcccgg
     9901 gtcgtcgccg cggccgacga acggctgggc ggcatcgacg tgctggtccc tgcggcgggc
     9961 gtggccgcgc agaagtcatg ggaggacgtc gacggggcgc tgtgggacga aaccatgacc
    10021 gtgaacgtcc gcgcgccgtt cttcctcgcc cagcagacgc tgccgcgtat ggtcgagcaa
    10081 cggttcggcc ggatcctgct gttctcctcg gtggccgcgt tcaccggcgg ggtgatcggg
    10141 ccgcactacg cggcggccaa ggcggcgctg cacggcctcg tgcacaacct ctcggcgaac
    10201 gttgccggtt ccggcgtcac catcaacgcg atcgccccgg ccctgatcgc cggcacccgg
    10261 atgctcccgc tcgggccgga cgatccggtg cccgcgcgaa ttcccgtcgg ccgcctgggc
    10321 accaccgagg aggtggccga cctctcgctc gccatgctcc gcaacggcta cctgaccagc
    10381 aaggtcatcg ctctcgacgg cggggcgtac ccggactgag gcatcgcccg ccggtcaggc
    10441 cggtggccgt accgggctcc cccggcgtcg cggaccatct ctgctcgaca tgcccgagcc
    10501 tccagatcgc gaacgcgacc agccacgcga tcgcgaagag cgcgacgagg ccgtacccgg
    10561 cgtcgttcag cgggatcgag gcgacggccg cgagcggccc cgagtcgatc cccgcctgct
    10621 gggtgatgac cgacaccagt tcgatcgtcc cgatgaccag agcgaccgtg acggacagcg
    10681 ccgtgatggt gatgttgtag aacaccttgc ggacgggcct ggcgaacgcc cagccgtagg
    10741 cgaagttcat gaacagaccg tcgagagagt cgagcaggct catgcctgcg gcaaacagga
    10801 tcgggagcac gaggatcgca tagaacggca gactgaacgc ggcagccccg cccgccagca
    10861 cgaggagtcc cacctcggtg gcggtgtcga atcccagccc gaacagcacc ccgaccgggt
    10921 agatgtgcca cggtttcgtg atcgaccggg tcagcccgtt gaggaaccgg ttcatgaacc
    10981 cgcgtttgtt gagctgttcc tcgagctcct gctcgtcgta ctccccggtc cgcatcctcc
    11041 gaaagatccg gatgatcccg atcagcacga cgaggttgag gatccccagg atccacagga
    11101 ac
//
LOCUS       DASRSC010000183        13331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2684668,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000183 DASRSC010000000
VERSION     DASRSC010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 13331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13331
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2684668"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..2470
                     /gene="treY"
                     /locus_tag="VFE39_09140"
     CDS             71..2470
                     /gene="treY"
                     /locus_tag="VFE39_09140"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HET6258803.1"
                     /translation="MTGSAPHRPSVPSATYRLQFSTDTTFADAVQLLPYLDALGVGAL
                     YASPLLESGARSNHGYDVVDPTRVSAERGGEEGRRALMTAVRERGLQLVLDIVPNHVG
                     VEVAKANPWWWDVLARGRSSQYASYFDIDWDAGPVLLPILDADEERALKELALSDDRS
                     ELRYYEHAFPVAPGTGDAGSAQEVHERQHYRLVSWKRGAAELTYRRFFDVSTLAALRV
                     EIPEVFEATHREVLRWVDAGEVDGIRVDHPDGLSDPGGYTRRLRAAIGPDRWLLVEKI
                     LGVGEELPSSWPVNGTSGYEALREIQGVFVDPGGGGLLTQFTAEHVGHKESLHAAEHA
                     ARREVADTILAAEVRRIAEACLSEPSISTALVPVPEAAQSTDEEPGETADRVRTAVAE
                     LLCAFPVYRSYLPEGRSALETAVSVARTHRPDLDDVLRAIRAAMLAEPQGLLATRVQQ
                     TSGMVMAKGVEDTTFYRWNRFVALNEVGGDPSRFGVSPTEFHRAMAAREASWPATMTT
                     LSTHDTKRSEDVRARLAVIAEIPGEWIERMRHWAAAHPLPDRSLELLAWQNLVGAWPI
                     SAERFAGYLSKASKEAKLVTSHVEPVPEVDEAIAAWSEQVLADTELVAEIEEFVARIS
                     APGWSNSLGQKLLQIAGPGVPDVYQGTELFEYSLVDPDNRRPVDWAQRRQLLAGLDEG
                     QLPDVDASGAAKLLVTASALRLRRYRPEVFTGYRPIHAEGPAADHAIAFARSASLVAV
                     ATRLPLGLAARGWDDTVLPLPEGATDWYDVISGAAVDGSAPSLAGVLERYPVALLVRP
                     A"
     gene            2567..4306
                     /gene="treZ"
                     /locus_tag="VFE39_09145"
     CDS             2567..4306
                     /gene="treZ"
                     /locus_tag="VFE39_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HET6258804.1"
                     /translation="MTEFAVWAPQHDQVRVLVDGTTHPMSAEGGGWWRTTVEDAAPGS
                     AYAFLLDDDETPLPDPRSRWQPTGVHGASRVYDDADFFWTDRSWTGRQLPGSVLYELH
                     IGTFTPDGTFDSAVERLDHLAALGIDIVEVLPINAVDGPRNWGYDGVGWYSVTENYGG
                     PDAFKRFVDACHARGLGVVLDVVYNHLGPSGAYLDRFGPYFAGSNIWGPSLNLDGAYS
                     EPVRRYVIENALMWLRDFHVDGLRLDAVHALRDSRAIHVLEQLAVEVEALSTHVGRPL
                     SLIAESDLNDPRLITAREAGGYGLHAQWADDIHHTLHAVLTGEAQGYYADFAIAGLTG
                     LAHVFTRAFLHEGTWSSFRQRNHGAAVDTRRIPGHRFVAYLQNHDQVGNRATGDRLAE
                     TLSPGLLACGAALVFGSPFTPMLFMGEEWGARTPWQFFSYFPDPALQDAVRDGRTAEF
                     AEHGWGDAEVPDPNADATFLDSKLDWDEAADEPHATLLRVHRELIALRKAWPELSDPW
                     LDEVEVEIDEVARTVVLHRGGLRVACNLGSDAVTIALGTPIGRILLASEPVEGETDAL
                     SLPPESFAIVQLG"
     gene            complement(4356..4979)
                     /locus_tag="VFE39_09150"
     CDS             complement(4356..4979)
                     /locus_tag="VFE39_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6258805.1"
                     /translation="MNRSDAADRLLAAAEKLYYARGIHAVGMDDVRAEAGVSLKLLYQ
                     LFPSKEHLVAAYLDARDEGWRGRLADHVAATGGSARDQLLAVFDWLGLWFAEPGFRGC
                     AFINAFGELGGSAPLVIAVVRRHKGLFREYLLDLARGLDVPDPAGVAEQLLLLAEGAM
                     TAAAVLPELRAARQAHRAAAALLDASLPSGPPADAGARRRATQSSSA"
     gene            5078..5536
                     /locus_tag="VFE39_09155"
     CDS             5078..5536
                     /locus_tag="VFE39_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6258806.1"
                     /translation="MAGRPPFPPFDALTAARKVQAAEDAWNTCDPERVSLAYTEDSVW
                     RNRDRHVVGRGAIVAFLTEKWQRELDYTLRKELWAFEGNRIGVRFQYECHDASGQWFR
                     SYGNELWEFSEDGLMRRREASINDRPISESERRIFGPRPTEEYGVPFPLW"
     gene            complement(5588..6583)
                     /locus_tag="VFE39_09160"
     CDS             complement(5588..6583)
                     /locus_tag="VFE39_09160"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HET6258807.1"
                     /translation="MSASTSSPVTVTVTGAAGQIGYALLFRIASGQLLGPDTPVRLRL
                     LEIPQAVKAAEGTAMELDDCAFPLLAGIAITDDATVAFDGANVALLVGARPRTKGMER
                     GDLLEANGGIFAPQGRAINAGAADDIRVLVVGNPANTNALIAQAAAPDVPAERFTAMT
                     RLDHNRAVAQLAKKLSVPVGEITKLTIWGNHSATQYPDLYHAEVGGKIAAEQVDDAWL
                     RDDFIPTVAKRGAAIIEARGASSAASAANAAIDHVFDWVNGTPEGDWTSAAVPSDGSY
                     GVPEGIISSFPVTAKDGKWEVVQGLEINDFSRERIDASVAELVEERDAVKSLGLL"
     gene            6747..7949
                     /locus_tag="VFE39_09165"
     CDS             6747..7949
                     /locus_tag="VFE39_09165"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HET6258808.1"
                     /translation="MTAGASSPTDPGATPTAARSLAPDLARGGMLLLIALANVHIYIF
                     GHPIGLRGYPTALDGVDQVVALLQMTLVDGRAYPLFGLLFGYGIGQLARRRSAVGMPA
                     PAVTKLVRRRGAWMIVIGMAHGVLLWPGDIIGCYGLLAVLMAGLLVRGTDRALLVTAM
                     IGVAFVTLVFAASALPMPDSQAFLPSMAIESPAAALAVRAVEWLGVGFVFQPFGVFGA
                     VALGAWAARRRLLDEPERHRPLLVRVAVLGLAAAVLLGLPMALMTAQLWTAPPLGMML
                     LAGSLHALGGYAGGIGYAAAFGLLAIRLARRGGPGLVTHALQACGQRSLSCYLAQSVA
                     FVALLPAWTLGLGEDAHLWQTALVAVGTWLVILLVAAASDRAGYRGPAEILLRRLTYG
                     PRRAPQHA"
     gene            8175..8630
                     /locus_tag="VFE39_09170"
     CDS             8175..8630
                     /locus_tag="VFE39_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258809.1"
                     /translation="MSPAQLPSDPPGPEGPGSERPPSWMGAPGYSPYRDDLPAHAPMP
                     GAPPYPSAEEHPPPPIGRPAARTGFRAALGAAVWAGVNLALVLIVTGAPPSTTDVARF
                     LGALLVPTLLAALAVWLIARGRAWPFWLLVLAAAPFFWVLRAVAAQAGG"
     gene            8866..9036
                     /locus_tag="VFE39_09175"
                     /pseudo
     CDS             8866..9036
                     /locus_tag="VFE39_09175"
                     /inference="COORDINATES: protein motif:HMM:NF044751.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9011..9490)
                     /locus_tag="VFE39_09180"
     CDS             complement(9011..9490)
                     /locus_tag="VFE39_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET6258810.1"
                     /translation="MTWENPSATRRRQILRATRTVAVVGASPNPARASNFVATYLLAS
                     TDFDVYFVNPNASEILGRPVYKRLADLPVVPDLVDVFRRREDLPGVLDDVLALDGVQT
                     LWLQFGLFDEDVAHRAEDAGLEVVMHRCLKVEHARFHGGLHLAGFDTGVISARRRTR"
     gene            complement(9487..10788)
                     /locus_tag="VFE39_09185"
     CDS             complement(9487..10788)
                     /locus_tag="VFE39_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HET6258811.1"
                     /translation="MSDHDRRWGFRTRAVHAGHVPDSATGARAVPIYQSTSFVFEDTT
                     DAASLFALQKYGLIYSRIANPTVAVLEERLASLDGGIGAVATSSGQAAEFLTFACLAG
                     AGDHIVAAAGLYGGTITQLDVTLRRFGVDTTFVPGGDPAAFAAAITPRTKLLFAEIIS
                     NPGGEVADIAGLADVAHAAGLPLVVDATLATPYLCRPIEHGADIVIHSATKFLGGHGT
                     TLGGVVIESGRFDWGNGNFPQMTEPVPSYGGITWWGNFDEFGFLTKLRAEQLRDVGAS
                     LSAHSAFLLIQGVETLPQRMEAHMAGARAVAEWLHADPRVSYVRWAGLPDHPDHERAA
                     RYLPKGPGAVFSFGVQGGREAGQRFIESVQLCSHLANVGDARTLVIHPGSTTHQQLTD
                     DQLRDAGVPPDLVRISVGLEDVEDILWDLDQALAAASKVES"
     gene            complement(10819..11523)
                     /locus_tag="VFE39_09190"
     CDS             complement(10819..11523)
                     /locus_tag="VFE39_09190"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6258812.1"
                     /translation="MQTEVMFTASGGTTLPGVLTVPDGAAGPGPALVMIYEVFGMTDE
                     MRRVARDLAADGYTVLIPDLFARGPAKPICVARALRTTLRGEGRELDDLEAARRWLAD
                     RPEVDGDRIGTIGFCLGGSFALLLARTGRYKVSAPFYSGRIDLPRSCPVVASFGGKDL
                     STRGYPERLDARLQAMDVPHDVVTYPDAGHSFFTRTPGVMGTVAARLPIHAEYHAPSA
                     EDAHRRIVAFFREHLG"
     gene            complement(11541..12953)
                     /locus_tag="VFE39_09195"
     CDS             complement(11541..12953)
                     /locus_tag="VFE39_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258813.1"
                     /translation="MSDIRWEGYSHEEIYAAVHQGPGRSISAAAEAAWAATEALILRI
                     DERIAAAMAQSAGGWEGSAAEATRTAMTPLGQWALDAANDAKITAQAVTTQGEQAREL
                     RNAMPEPNTAQLEAEYGRALTDPTYIFHGRDDLQAAEQESANRAARAIDLMNGYTNNS
                     YENRSHMDYWTLPPQVTVETTAPAPPAGSGAAGSAIGAFGAGGGPGAAGVAVAGTNPA
                     VGVGGGLSAPVAGAALPAGPAGVGPGGAPSASGTAGGGAGPGLVPSVVGSGSAGVGGG
                     ARRRAGGTPGDAAGRGGGMGPPTTRGTGSGTRGPSRDGGLAPPVGTSRPSPGGPRDGV
                     RPVVPGPPPRGGPAPNWRDLVPAGPGRQPGAPGPWARSDEDRRLLPPGGSRAAGEPPA
                     RPPGGDRPATDPASRAGPTARTTSGHPGLYPPMTAGAAGGPDQERRRPDYLIDDTDAF
                     ADDRWFTPGVIGADDPLPPR"
     gene            complement(12950..13285)
                     /locus_tag="VFE39_09200"
     CDS             complement(12950..13285)
                     /locus_tag="VFE39_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258814.1"
                     /translation="MPGQYPPVTLRLVPHTIPAVRAAVDDSLAELVPQLIRLRQDGVI
                     PEPWLGDPMSRKVVDHYQAQVLNGPDSPYAALRAYEAELLKVRDNLQVLEDHYRRTEG
                     ENTALWGRA"
BASE COUNT         1775 a         4905 c         4711 g         1940 t
ORIGIN      
        1 ggggcggtgc ggtgcacacc gtcccggcgc ggtcggtgct ggtcctgcag cgcatcgaga
       61 acgccggcgc atgacgggct cggctccgca caggccgtcc gtgccctcgg cgacgtaccg
      121 cctgcagttc tccacggaca ccacgttcgc cgacgccgtc cagctgctgc cctacctcga
      181 cgcgctcggg gtgggtgcgc tgtacgcgtc gccgctgctc gagtccgggg cgcgctccaa
      241 ccacggctac gacgtggtcg accccacccg cgtgtcggcg gagcggggcg gcgaggaggg
      301 gcgccgcgcg ctcatgacgg cggtccggga gcgtggcctg cagctcgtcc tggacatcgt
      361 gccgaaccac gtcggcgtcg aggtggcgaa ggccaacccg tggtggtggg acgtgctcgc
      421 gcgcggccgc tcgtcgcagt acgcgtcgta cttcgacatc gactgggacg ccgggccggt
      481 gctcctgccc atcctcgacg ccgacgagga gagggccctc aaggagctgg cgctctcgga
      541 cgaccggtcg gagctgcggt actacgagca cgcgtttccc gtggcgcccg gcaccggaga
      601 cgccggctcg gcgcaggagg tccacgagcg gcagcattac cggctcgtgt cctggaagcg
      661 gggcgcggcc gagctgacct accgccgctt cttcgacgtg tccaccctcg ccgccctgcg
      721 cgtcgagatc ccggaggtgt tcgaagccac ccatcgcgag gtgctgcgct gggtcgacgc
      781 cggcgaggtc gacggcatcc gggtcgacca ccccgacggc ctgtccgatc ccggcggcta
      841 cacgcggcgg ctacgagcgg cgatcggtcc cgaccgctgg ctgctggtgg agaagatcct
      901 cggcgtcggt gaagagctgc cctcatcgtg gccggtcaac ggcacctccg ggtacgaggc
      961 gctacgcgag atccagggcg tcttcgtcga tccgggcggc ggcgggctgc tcacccagtt
     1021 cacggccgag cacgtcggcc acaaggagtc gctgcacgcg gcggagcacg cggcccgccg
     1081 cgaggtagcc gacaccatcc tcgcggcgga ggtgcgccgg atcgccgagg cttgcttgtc
     1141 ggagccgagc atcagcaccg cgctggtgcc cgtgcccgag gcggcacagt ccacggacga
     1201 ggagcccggc gagacggccg accgcgtgcg cacggccgtc gccgagctgc tgtgcgcatt
     1261 tccggtctac cggagctacc tgcccgaggg ccgctcggct ctcgagaccg cggtgtcggt
     1321 ggcccgcacc caccggcccg acctcgacga cgtgctgcgc gccatccgcg ccgcgatgct
     1381 ggccgagccg cagggcctcc tcgccacccg cgtgcagcag acgtcgggca tggtgatggc
     1441 caagggcgtc gaggacacca cgttctaccg ctggaaccgc ttcgtcgcgc tcaatgaggt
     1501 gggcggcgac ccgtcccgct tcggcgtctc ccccaccgag ttccaccgcg ccatggctgc
     1561 ccgtgaggcc tcctggccgg ccaccatgac gaccctgtcc acgcacgaca ccaagcgctc
     1621 ggaggacgtc cgcgcccggc tcgcggtgat cgccgagatc ccgggcgagt ggatcgagcg
     1681 gatgcgtcac tgggccgccg cgcacccgct ccccgaccgg tcgctggagc tcctcgcctg
     1741 gcagaacctc gtgggtgcct ggccgatctc cgccgagcgg ttcgccggct acctgagcaa
     1801 ggcgtccaag gaggccaagc tcgtcaccag ccacgtcgag cccgtacccg aggtggacga
     1861 ggcgatcgcc gcctggtcgg agcaggtgct ggccgacacg gagctggtgg ccgagatcga
     1921 ggagttcgtc gcgcggatct ccgcacccgg ctggtccaac tcgctcgggc agaaactgct
     1981 gcagatcgcc ggacccggcg tgccggacgt ctaccagggc acggagctgt tcgagtactc
     2041 cctcgtcgac ccggacaacc ggcgcccggt cgactgggcg cagcgccggc agctgctcgc
     2101 cggcctcgac gagggccagc tgccggacgt cgatgcctcg ggagcggcga aactgctggt
     2161 cacagccagt gcgctgcgac tgcgtcgcta ccggcccgag gtgttcaccg gctaccggcc
     2221 gatccacgct gagggtccgg cggccgacca tgccatcgcg ttcgcccgct cggcctcact
     2281 ggtcgcggtg gccacgcggc ttccgctcgg tctggcagcg cgcggctggg acgacacagt
     2341 gcttccactg ccggaaggtg ccaccgactg gtacgacgtg atcagcgggg ccgccgtcga
     2401 cggctccgcg ccgtcgctgg ccggggtgct ggagcgctat ccggtggcgc tgctggtgcg
     2461 gccggcatag gggcctgggg gccgcttggc cccctcgatc atgatgtgct gagtcacacc
     2521 cctcacaccg ggcgcggcga ggtgacgccc ggcaggatca tgtctcgtga ccgagttcgc
     2581 cgtgtgggcg ccgcagcacg atcaggttcg ggtgctggtc gacggcacga cgcatcccat
     2641 gtccgcagag ggcgggggct ggtggcgcac caccgtcgag gatgccgccc ccggttccgc
     2701 ctacgccttc ctcctcgacg atgacgagac cccgctcccc gacccgcggt cgcgctggca
     2761 gcccaccggc gtgcacgggg catcccgggt gtacgacgac gcggacttct tctggaccga
     2821 ccgctcctgg accggccggc agctgcccgg cagcgtgctc tacgagctgc acatcggcac
     2881 gttcaccccc gatggcacgt tcgactccgc cgtcgagcgg ctcgaccacc tcgcggcgct
     2941 gggcatcgac atcgtcgagg tgctgccgat caatgccgtc gacggacccc ggaactgggg
     3001 ctacgacggg gtgggctggt attcggtcac cgagaactac ggcggccccg acgcgttcaa
     3061 gcggttcgtc gacgcctgcc atgcccgtgg gctgggcgtc gtgctcgacg tggtctacaa
     3121 ccacctcggc ccgtcggggg cctacctgga ccggttcggc ccgtacttcg ccggcagcaa
     3181 catctggggc ccctcgctga acctcgacgg tgcgtactcg gaacccgtgc gccgatacgt
     3241 catcgagaac gctttgatgt ggctgcgcga cttccatgtc gacgggctcc gcctcgacgc
     3301 cgtgcacgcc ctgcgcgact cgcgtgccat ccacgtgctc gagcagctcg ctgtcgaggt
     3361 cgaggcgctg tcgacacacg tggggcggcc actgtcgctc atcgccgagt cggacctcaa
     3421 cgacccgcgg ctgatcaccg cacgcgaggc gggcggctac ggcctgcacg cccagtgggc
     3481 cgacgacatc caccacaccc tgcacgccgt gctcaccggc gaggcgcagg gctactacgc
     3541 ggacttcgcg atagccggtc tcacggggct ggcacacgtg ttcaccaggg cgttcctcca
     3601 cgaaggcacg tggtcgagct tccggcagcg caaccacggc gcagccgtgg acacccgccg
     3661 catccccggc caccgcttcg tggcctacct gcagaaccac gaccaggtcg gcaaccgtgc
     3721 caccggggac cgactcgccg agacgctctc ccccggcctg ctcgcctgcg gcgccgcact
     3781 ggtgttcggc tcaccgttca cgcccatgct gttcatgggc gaggagtggg gcgcgagaac
     3841 gccgtggcag ttcttctcct acttcccgga tccggcgctg caggacgcgg tgcgagacgg
     3901 ccgtaccgct gagttcgccg agcacgggtg gggcgacgcc gaggtgcccg accccaacgc
     3961 ggatgccacg ttcctcgact cgaagctgga ctgggacgag gccgcggacg agccgcacgc
     4021 cacgctgctg cgggtccacc gcgagctcat cgcgctgcgg aaggcatggc cggagctgtc
     4081 cgacccgtgg ctggacgagg tcgaagtgga gatcgacgag gttgcccgca ccgtggtgct
     4141 gcaccgcggc gggctccggg tcgcgtgcaa cctcggcagc gacgcggtca cgatcgcgct
     4201 cggcacgccg atcggccgga tcctgctggc gtcggagccc gtggagggtg agaccgatgc
     4261 gctcagcctg ccaccggagt cgttcgcgat cgtgcagctg ggctgatccg gcgctgatcc
     4321 ggttccccct caggcggtgc ggagcgaccg gcgcgtcagg cagaactcga ctgcgtcgca
     4381 cgtcgtcgag caccggcgtc ggcaggcggc ccgctcggca gtgaggcgtc gagcagagcg
     4441 gccgcggccc ggtgggcctg ccgtgccgcc cgcagctccg gcagcaccgc cgccgcggtc
     4501 atcgccccct ccgccagcag cagcagctgc tcggcgacac ccgccgggtc gggtacgtcg
     4561 agaccgcgcg cgagatcgag caagtactcc cggaagagcc ccttgtgccg gcgcacgacg
     4621 gcgatgacca ggggcgcgct gccgcccagc tccccgaagg cgttgatgaa cgcacagccc
     4681 cggaagcccg gctcggcgaa ccagaggccc agccagtcga agacggcgag cagctgatcc
     4741 cgcgcgctcc cgccggtggc cgcgacatgg tcggcgagcc gccctcgcca gccctcgtcg
     4801 cgcgcgtcca ggtacgccgc gaccaggtgt tccttcgacg ggaagagctg gtagagcagc
     4861 ttgagcgaga cgccggcctc cgcgcggaca tcgtccatgc cgacagcgtg gatcccgcgc
     4921 gcgtagtaca gcttctccgc cgccgcgagc agccggtctg ccgcgtcact gcgattcaca
     4981 ccactcctcc accccgccga gaacgaacgt tctctacgct atcgcacgcc ggaaaacggc
     5041 cgttctccgg tgaggccccg acgaggagaa ccgatccatg gccggtcgtc cacccttccc
     5101 cccgttcgac gccctgacag ctgcgcggaa ggtccaagcc gcggaggacg cctggaacac
     5161 ctgcgatccc gagcgggtct cgctggccta cacagaggac tcggtgtggc gcaaccgcga
     5221 ccggcacgtg gtaggccgtg gcgccatcgt ggcgttcctg accgagaagt ggcagcgcga
     5281 gctcgactac acactgcgca aggagttgtg ggcgttcgag ggcaaccgga tcggcgtccg
     5341 gttccagtac gagtgccacg acgcgtccgg ccagtggttc cgcagctacg gcaacgagct
     5401 gtgggagttc tccgaggacg ggctgatgcg ccgccgggag gccagcatca acgatcgccc
     5461 gatctccgag tcggagcggc gcatcttcgg cccgcgcccc accgaggagt acggagtgcc
     5521 gttcccgctg tggtgaccac cacgggtggg cgccgtcaag gccgcccacc ccgaccgcag
     5581 atcgagatca gagcaggccg aggctcttca cggcgtcgcg ctcctcgacc agctcggcca
     5641 ccgaggcgtc gatcctctcg cgcgagaagt cgttgatctc caggccctgg acgacctccc
     5701 acttcccgtc cttcgccgtg accgggaacg aggagatgat gccctcgggc acgccgtagg
     5761 agccgtccga cgggacggcc gcggaggtcc agtccccctc cggggtgccg ttgacccagt
     5821 cgaagacgtg gtcgatcgcc gcgttggcag ccgacgccgc cgacgaggca ccgcgcgcct
     5881 cgatgatcgc cgcaccgcgc ttggcgaccg tcgggatgaa gtcgtcacgc agccaggcgt
     5941 cgtccacctg ctcggcggcg atcttgcccc cgacctcggc gtggtagagg tccgggtact
     6001 gagtggccga gtggttgccc cagatcgtca gcttcgtgat ctcgccgacc ggcaccgaca
     6061 gcttcttcgc gagctgggcg acagcgcggt tgtggtcgag ccgggtcatc gcagtgaagc
     6121 gctcggccgg cacgtccggc gcggcggcct gggcgatcag ggcattggtg ttggccgggt
     6181 tacccaccac gagcacgcgg atgtcgtcgg cggcaccggc gttgatcgcg cggccctgcg
     6241 gcgcgaagat cccgccgttg gcctccagga ggtcaccgcg ctccatgccc ttggtacgcg
     6301 ggcgggcgcc gaccaacagt gcgacgttcg cgccgtcgaa cgccacggtg gcgtcgtcgg
     6361 tgatggcgat gccggcgagc agcgggaagg cgcagtcgtc gagctccatc gcggtgccct
     6421 cggcggcctt caccgcctgc gggatctcca acaggcgcag gcggaccgga gtgtccggcc
     6481 cgagcagctg cccggaggcg atccggaaga gcagcgcgta gccgatctgg ccggcggctc
     6541 cggtgacggt gacggtgacg ggggatgagg tcgacgcaga cacgctcggg aggctagccc
     6601 tccgccggcc tcggcgccgt cacggggagc acgttgaggg gccggagtgg gcagcggcac
     6661 accgaccccg ccaaggggcg gacaccggat ctgcgccggt cgcccagtgg acctccggcc
     6721 cggtccgtcc tcattatcct tttcgcatga ccgccggcgc gtcctcaccc accgaccccg
     6781 gcgccacccc caccgccgca cgctccctgg ccccggacct ggcgcgcggc ggcatgctct
     6841 tgctcatcgc gctcgccaac gtgcacatct acatcttcgg gcaccccatc ggtctacgcg
     6901 gctacccgac ggcgctcgac ggcgtggacc aggtggtggc actgcttcag atgaccctcg
     6961 tcgacgggcg ggcctatccg ctcttcggtc tgctcttcgg ctacgggatc gggcagctcg
     7021 cccggcggcg cagtgcggtg ggcatgccgg cgcccgccgt cacgaagctc gtgcggcggc
     7081 gcggcgcctg gatgatcgtc atcggcatgg cccacggggt gctgctctgg cccggcgaca
     7141 tcatcggctg ttacgggctg ctggccgtgc tgatggccgg cctgctcgtg cgcggcaccg
     7201 accgcgcgct gctcgtcacc gccatgatcg gggtggcatt cgtaacgctg gtcttcgccg
     7261 cgagcgccct cccgatgccc gactcgcagg ccttcctgcc ctcgatggcc atcgagagcc
     7321 cggctgccgc cctggccgtg cgcgccgtcg agtggctcgg cgtcgggttc gtgttccagc
     7381 cgttcggggt gttcggcgcg gtggctctcg gagcctgggc cgctcgacgg cggctgctcg
     7441 acgaaccgga gcggcaccgg ccgctgctgg tacgcgtggc cgttctcggg ctcgcggcag
     7501 cggtgctgct cgggctgccc atggccctga tgaccgcgca gctgtggacg gcgccgccgc
     7561 tcggcatgat gctgctcgca ggctcgctgc acgccctggg cgggtatgcg ggcggcatcg
     7621 ggtacgccgc ggccttcggc ctcctcgcga tccggctcgc ccggcgcggc ggtcccgggc
     7681 tggtcaccca cgctctccag gcctgcggac agcggtcgtt gtcctgctac ctcgctcagt
     7741 cggtggcgtt cgtggccctg ctcccggcct ggacactcgg cctcggcgag gacgcgcacc
     7801 tgtggcagac cgccctggtc gcagtgggca catggctggt gatcctgctg gtggccgcag
     7861 cctcggatcg ggccggctac cgcggcccgg cggagatcct gctgcggagg ctgacgtacg
     7921 gcccacgccg cgccccccag cacgcatgag caacgcgccg cccctcggtg ggccgggcgt
     7981 taccgtctct ccgctgaggc ccgctcagcc gccgcccgaa ccatccggtc ccaaggagcc
     8041 ccaacaggcc acgcgtcggt ggaccccggg ctaccggtct ttccggtgtg ccccgctcaa
     8101 ctgccgtccg atcccggtcc cgagtagccc ctaacggacc acgcggcggt ggaacgcggg
     8161 ctaccgtctt tcccgtgagc cccgctcaac tgccgtctga tccacccggt cccgaggggc
     8221 cagggtccga gcgtcctccg tcgtggatgg gcgcacccgg ctactcgccc tatcgcgacg
     8281 acctaccggc ccacgccccg atgccgggcg ctccgcccta cccctcggcc gaggagcacc
     8341 cgccgccgcc gatcgggcgg cccgcggcac gcaccggctt ccgcgcggcc ctcggggcgg
     8401 cggtgtgggc gggggtcaac ctggcgttgg tgctcatcgt caccggagcg cccccctcga
     8461 ccacggacgt tgcccggttc ctcggcgccc tgctcgtccc caccctgctc gccgcgcttg
     8521 cggtgtggtt gatcgcccgc gggcgggcct ggcccttctg gctgctggtg ctggccgccg
     8581 cgccgttctt ctgggtgctg cgggcggtgg ccgcgcaggc cggcggttga cgtcgatcag
     8641 gcccggtagg ccacgagccc accacggagc agtccgccgc ggtctgcagg gacaggtcga
     8701 tcattcggga gcccaccagc gggtcggggt cgtcgaggtc gtagcgcagg tccaggccgg
     8761 acatcatccc gacgaggtag cggccggcat ccggcagccg ggccaggtcg acgaagatat
     8821 cgcgcagcgc ccacgcgtcg accacgtcgt cgccgacgcg tcgactcgcc cgtgggcacc
     8881 gcgctcggag ccgcgcccgg tgtcgaccgg gtgctggtcc gcccggacgg ctacgtctgc
     8941 tggaccggcg ccggcccggc ggcgtcgccc gcggccgcgc tgcactgctg gttctgcgcc
     9001 gccgtgccgg tcaccgcgtc cgccgccgcg cgctgatcac accggtgtcg aagcccgcga
     9061 ggtgtaggcc gccgtggaag cgggcgtgct ccaccttcag gcagcggtgc atcacgacct
     9121 ccagccccgc gtcctccgcg cgatgggcga cgtcctcgtc gaagagcccg aactgcagcc
     9181 acagtgtctg cacgccgtcg agcgcaagca cgtcgtcgag aacacccggc aggtcctccc
     9241 ggcggcggaa gacgtccacg aggtcaggca ccacgggcag gtcggcgagg cgcttgtaca
     9301 ccggacggcc gaggatctcg gacgcgttcg gattgacgaa gtacacgtcg aagtcggtgc
     9361 tcgccagcag gtaggtggcg acgaagttgc tggccctcgc cgggttcggg gaggccccca
     9421 ccacagcgac ggtgcgcgtg gcgcgcagga tctgcctgcg ccgggtcgcc gaggggttct
     9481 cccaggtcac gattccacct tcgatgcagc agcgagggcc tggtccaggt cccacaggat
     9541 gtcctcgacg tcctccagcc cgacgctgat ccgcaccagg tccggtggca cgcctgcgtc
     9601 gcgcagctgg tcgtctgtca gctgctggtg cgtggtggag ccggggtgga tcacgagcgt
     9661 cctcgcgtca cccacgttgg cgaggtggct gcacagctgc accgactcga tgaaccgctg
     9721 tccggcctcg cgcccgccct gcacgccgaa cgagaacacg gcacccggcc ccttgggcag
     9781 gtagcgtgcg gcccgctcgt ggtcgggatg gtcgggcagc ccggcccaac ggacgtagga
     9841 cacccgaggg tcggcgtgca gccattccgc caccgcgcgg gcgccagcca tgtgcgcctc
     9901 catccgctgc ggcagcgtct cgacgccctg gatcagcagg aacgcgctgt gcgccgacaa
     9961 cgaggcgccg acgtcgcgca gctgttccgc acggagcttc gtcaggaagc cgaactcgtc
    10021 gaaattgccc caccaggtga tgccgccgta ggacggaacc ggctccgtca tctgcggaaa
    10081 gttgccgttg ccccagtcga agcgccccga ctcgatcacc acgcccccca gcgtcgtgcc
    10141 gtgcccgccg aggaacttcg tcgccgagtg gatcacgatg tcggcgccgt gctcgatcgg
    10201 gcggcacagg tacggggtgg cgagcgtcgc atcgaccacc agtgggagcc ccgctgcgtg
    10261 tgcgacgtcg gcgagccccg cgatgtcggc cacctcgcct ccggggttgg agatgatctc
    10321 cgcgaacagc agcttggtgc gcggcgtgat cgccgcggcg aacgccgccg ggtctccacc
    10381 gggtacgaac gtggtgtcga cgccgaaccg gcgcagcgtc acgtccagct gcgtgatcgt
    10441 gccgccgtag agccccgccg ccgccacgat gtggtcgccc gccccagcga ggcaggcgaa
    10501 cgtcaggaac tccgcagcct gcccgctgga ggtcgcgacc gccccgatcc caccgtccag
    10561 gctggcgagg cgttcctcca gcaccgccac ggtggggttg gcgatgcggc tgtagatcaa
    10621 gccgtacttc tgcagcgcga acaggctggc cgcgtcggtg gtgtcctcga agacgaagct
    10681 cgtggactgg tagatcggta ccgcacgggc ccccgtggcg gagtcgggga cgtgcccggc
    10741 gtgcaccgct cgggtgcgaa agccccagcg gcggtcgtga tcgctcatac cgtccatcct
    10801 gcccccgggc ggcgggaatc agccgaggtg ctcccggaag aacgccacga tccgccggtg
    10861 tgcgtcctcg gcgctcggcg cgtggtactc ggcgtgtatc ggcaggcgcg ccgcgacggt
    10921 gcccatcacg ccgggtgttc gggtgaagaa cgagtgccct gcgtcggggt aggtgacgac
    10981 gtcgtgcggc acgtccatcg cctggaggcg ggcgtcgagc cgttcggggt agccccgggt
    11041 ggacaggtcc ttaccaccga agctcgcgac gaccgggcac gagcggggga ggtcgatgcg
    11101 tccgctgtag aacggggcgc tcaccttgta ccgaccggtg cgcgcaagca gcagcgcgaa
    11161 gctgccaccg aggcagaacc cgatggtgcc gatccgatcc ccgtcgacct cgggacgatc
    11221 ggccagccag cggcgcgccg cctccaggtc gtcgagctcg cggccttccc cgcggagcgt
    11281 ggtgcgcagg gcccgggcca cgcagatcgg ctttgccggc ccgcgggcga acaggtccgg
    11341 gatgagcacg gtgtacccgt cggccgcgag gtcgcgcgcc acgcgccgca tctcgtcggt
    11401 catgccgaac acctcgtaga tcatcaccag cgcagggccg ggaccggcgg cgccgtcggg
    11461 cacggtgagc acgcccggca gcgttgtgcc gccgctcgcg gtgaacatga cctcggtctg
    11521 cacctgcgcg acggtaccgg tcagcgtggt ggaagtgggt catcggcgcc gatcacccct
    11581 ggggtgaacc accggtcgtc ggcgaaggcg tccgtgtcgt cgatgaggta gtcgggcctg
    11641 cggcgctcct ggtccggacc gccggccgcg cccgcggtca tcggcgggta gaggccggga
    11701 tgcccgctcg tcgtgcgggc cgtggggccg gcgcgagagg ccggatcggt ggcgggccgg
    11761 tccccgccgg gcggtcgagc cggtggttct ccggccgccc tgctcccgcc tggtggcagc
    11821 agtcgacggt cctcatcgct gcgcgcccat ggcccgggtg ctcccggctg ccgccccggc
    11881 ccggcaggca ccaggtcgcg ccaattcggg gcaggcccac cgcgcggggg cggaccgggg
    11941 acgaccgggc ggacgccgtc tcgcgggcca cccggactgg ggcgcgatgt gcccaccggc
    12001 ggggcgagcc ccccatcgcg ggacggcccg cgcgtgccgc tgcccgtacc tcgtgtggtg
    12061 ggcgggccca tccctccccc tctgccggcc gcgtcccccg gcgtgccacc cgcccgacgc
    12121 cgagcgcctc cgccgacccc ggcggagccc gacccgacca cggacggcac gagccctggc
    12181 cccgcgccac cgcctgcggt cccgctcgcg gagggagcac cgcccgggcc gacgcctgca
    12241 ggcccggccg gcagggccgc accggccacc ggagcagaca gcccgccacc gacaccgacc
    12301 gcggggttgg tccccgccac agcgaccccg gccgcacctg gtccgcctcc ggcgccgaac
    12361 gcaccgatcg ctgacccggc cgcaccacta cccgccggcg gtgcgggagc cgtcgtctcc
    12421 acggtcacct ggggcggaag ggtccagtag tccatgtggc tgcggttctc gtaggagttg
    12481 ttcgtgtagc cgttcatcag gtcgatcgcc cgcgcggcgc gattggcgct ctcctgttcc
    12541 gcggcctgta ggtcgtcccg cccgtggaag atgtacgtgg ggtcggtcaa ggcacggccg
    12601 tactccgcct cgagctgcgc cgtgttcggc tccggcatgg cgttgcgcag ctctcgcgcc
    12661 tgctcgccct gcgtggtcac cgcctgcgcc gtgatcttcg cgtcgttggc ggcatcgagc
    12721 gcccactggc cgagcggggt catggccgtg cgtgtggcct cggcagcgct gccctcccag
    12781 ccacccgcgg actgcgccat cgccgccgcg atgcgctcgt cgatccgcag gatcagcgcc
    12841 tcggtcgcgg cccacgcggc ctccgcagct gccgagatcg accggccggg gccctggtgc
    12901 accgcggcgt agatctcctc gtggctgtag ccctcccacc ggatatcgct cacgcccggc
    12961 cccacaaggc tgtgttttcg ccctcggtgc ggcggtagtg gtcctccaag acctggaggt
    13021 tgtcgcgcac cttgagcaac tcggcctcat aggcccgcag cgcggcgtac ggactgtcgg
    13081 gaccgttcaa gacctgcgcc tggtagtggt cgacgacctt cctgctcatc ggatcaccga
    13141 gccatggctc gggaatgacg ccgtcttgac ggaggcggat cagctgcggt accagctccg
    13201 caagtgagtc gtcgaccgcc gcccgcactg cgggaatcgt gtgcggcacc agccggagcg
    13261 tgacaggcgg atattggccg ggcatcgtac gctcctcagc tcacgggact tgctcgtcgg
    13321 ccacgctagg c
//
LOCUS       DASRSC010000184         5461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3178405,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000184 DASRSC010000000
VERSION     DASRSC010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3178405"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFE39_09205"
     CDS             complement(<1..188)
                     /locus_tag="VFE39_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258815.1"
                     /translation="MAGSVDLAGGSGYAVEDSRLRTSPPDVLRLAGEPLPLTIPARIY
                     VCGITPYDVTHLGHASTFV"
     gene            complement(288..1124)
                     /locus_tag="VFE39_09210"
     CDS             complement(288..1124)
                     /locus_tag="VFE39_09210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET6258816.1"
                     /translation="MSDPETWLGDAVRGPRELPPEIDTTIPSVARGYDYALGGKNNFE
                     IDRVAADAYTAVFPGAVALARHNRALLQRVVRYLVGEIGIGQIIDVGSGLPTVGNVHE
                     VAHRIDPDVRVVYVDIDPIVLAHARALLASNDTTIVIQADAAEPSSILDDPKTRELLD
                     FDKPFAVLMSGILHHLPDEKDPAGVAQAFKDAMPSGSYLLASNFLDDDDPRAKEAERS
                     ITGNFGTGRFRTWEEQRPFFDGLELVEPGLVYANDWRPGDEIEKDSPWHTFYCGGVGR
                     KP"
     gene            complement(1241..2110)
                     /locus_tag="VFE39_09215"
     CDS             complement(1241..2110)
                     /locus_tag="VFE39_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6258817.1"
                     /translation="MTRRLADTTGVQADPSNVEQLHAWDGDTGDFWAAHAERFDEGVA
                     AHHGHLLAAAGIDATENVLDIGCGAGQTTRDSARCASAGSALGVDLSSRMIELARRLA
                     QREQVANVTFQQADAQNHPFPDRSFDIAISRSGAMFFGDAPAAFANIARAVRDGGRLV
                     LLAWQPFEQNEWLRAFRTALAAGRDLPVPPPGAPGPFALSDPDRVRRVLASAGFVDVR
                     LHGLTEPMYFGPDPDDACRFVAGQQAGMLHDLDADGRARALDALRADMADHQTDRGVL
                     YESAAWLIEARRS"
     gene            complement(2178..2573)
                     /locus_tag="VFE39_09220"
     CDS             complement(2178..2573)
                     /locus_tag="VFE39_09220"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6258818.1"
                     /translation="MVLMADPVSLSVVRQRFRRWLAALCWPEEEVDDVVMAVNEAVSN
                     AIEQIHPSEIGAEVRVAARQYAELDGGRRILIAVAGGKLWSPEPASFPGQGLLMIHSC
                     MDDVGVSREHDGITMMMTSAKVPPLSRGA"
     gene            complement(2625..3002)
                     /locus_tag="VFE39_09225"
     CDS             complement(2625..3002)
                     /locus_tag="VFE39_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6258819.1"
                     /translation="MNGPLGGVPPAEQLMTINRDDRSGCVVLVVHGEVDLSTGSRLME
                     AGSEALHQAAGQPVVLDLSDVDFLSSSGMGLLVALHDEGRGMHTPLRVVVDEKQSVIR
                     PIRTMGLDEVLSLFGSLDEAITA"
     gene            3393..3812
                     /gene="rsfS"
                     /locus_tag="VFE39_09230"
     CDS             3393..3812
                     /gene="rsfS"
                     /locus_tag="VFE39_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET6258820.1"
                     /translation="MTATDEALDMARVAVAAAADKKAQDIVVLDVSDQLVITDCFVIA
                     SAPNERQVQAIVDEVEEKLRQHGAKPTRREGTREGRWVLLDFVDVVVHVQHTEERGFY
                     GLDRLWKDCPRVAFPEAETEAVPDGSAPVGVDGDGTG"
     gene            3809..4429
                     /locus_tag="VFE39_09235"
     CDS             3809..4429
                     /locus_tag="VFE39_09235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6258821.1"
                     /translation="MSLQRLVLLRHGQTDYNVAGRMQGHLDSVLTETGLAQAAGVAPE
                     IARFAPDRLISSDLRRAVDTADIVGAACSQPVKLDARLRETHLGEWQGCSVAEIEQAW
                     PGAIAAWRSDPAWTPPGGESRIDVVRRSQPVVDELDEEFASAPDGTVVLVAHGGLIAG
                     LVSGLLALPTSAWPSIGGIGNCHWAALARRADHPRWRLVGYNVGTW"
     gene            4543..5277
                     /gene="octT"
                     /locus_tag="VFE39_09240"
     CDS             4543..5277
                     /gene="octT"
                     /locus_tag="VFE39_09240"
                     /EC_number="2.3.1.273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016414 - O-octanoyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosylglycerate octanoyltransferase"
                     /protein_id="HET6258822.1"
                     /translation="MGGPILLVLADSLAFHGPERAEPADEPRLWPNVAATALGGRAEL
                     VAGIGWTARHAWHALTHDPRVWALMPTVDALVLGVGGMDTLPSPLPTAVRELIPALRP
                     DGLRRAVRAGYLRAQPPLARTLASVVPGGGPVALPPRLTVRYLERCRRAVLALRPGIP
                     VVAVLPPVHRAAVYGSVHAGRAAAEYATRAWARGCGAALLDLRALVGEHVLGGHGNPD
                     GMHWGWQAHAAVGAACAELLHPRVER"
     gene            5211..>5461
                     /locus_tag="VFE39_09245"
     CDS             5211..>5461
                     /locus_tag="VFE39_09245"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET6258823.1"
                     /translation="MAGARGGRCGVRGVAAPQGGALTGVPAIGCVVVPVAVVTDSTAY
                     LPAGVAEEREIRVVPLEVRLGQRVGREGCDIGITELSAAL"
BASE COUNT          770 a         1926 c         1950 g          815 t
ORIGIN      
        1 acgaacgtcg atgcgtggcc caggtgcgtc acgtcgtagg gggtgatccc gcagacgtag
       61 atccgggcag ggatcgtcag cggcaatggc tcgccggcca gtcgcagcac gtcgggcgga
      121 ctcgtccgca ggcgggaatc ctcgacggcg tagcccgaac cacctgcaag atccaccgat
      181 ccggccatcg ccccatggtg gcacggtgtg cccggatcga cgagccacca ggagggctcg
      241 ctactcctgc cggcgtaagt aacttgcgac gcgggccgcc cgcgcgatca cggcttgcgg
      301 ccgacacctc cgcagtagaa cgtgtgccac gggctgtcct tctcgatctc gtcgcccggg
      361 cgccagtcgt tcgcgtagac cagcccgggc tccaccagtt ccaacccgtc gaagaacggc
      421 cgctgctcct cccaggtgcg gaaccgcccg gtgccgaagt tgccggtgat cgagcgttct
      481 gcttccttcg cccgcgggtc gtcgtcgtcg aggaagttgc tcgccagcag gtagctaccg
      541 gagggcatcg cgtccttgaa cgcctgtgcc acgccggccg ggtccttctc gtcgggaagg
      601 tggtgcagga tcccggacat gaggacggcg aagggcttgt cgaagtcgag gagctcacgg
      661 gtcttcgggt cgtcgaggat cgaactcggc tccgccgcgt cggcctggat gacgatcgtg
      721 gtgtcgttgg acgcgagcag cgctcgggcg tgcgcgagca cgatcgggtc gatgtcgacg
      781 tagacgacgc gcacgtccgg gtcgatccgg tgcgccacct cgtgcacgtt gcccacggtg
      841 ggcaggcccg atccgacgtc gatgatctgg ccgatcccga tctcgcccac caggtaccgg
      901 acgacacgct ggagaagtgc cctgttgtgc ctggccagcg cgaccgcacc agggaacacc
      961 gcggtgtagg cgtcggcagc gacgcggtcg atctcgaagt tgttcttgcc gcccagcgcg
     1021 tagtcgtagc cccgcgccac cgaagggatg gtggtgtcga tctcgggggg cagttcccgg
     1081 gggccgcgca ccgcgtctcc cagccacgtc tctggatcgc tcactggccc tcgtccttcg
     1141 ctcgtcccgg atcacccgtg cagcggactc agcgtagtgc tccaggcgcg aatcgtgatc
     1201 atgtttctcg tcggatgtgg tgaccggtgc gggctctcgg tcagctgcgt cgcgcctcga
     1261 tcagccaggc cgccgactcg tacagcacac ctcgatcggt ctggtgatcc gccatgtcgg
     1321 cccgcagggc gtcgagtgcg cgggccctgc cgtcggcgtc gaggtcatgc agcatcccgg
     1381 cttgctggcc ggcgacgaac cggcaggcgt cgtcggggtc ggggccgaaa tacatgggct
     1441 cggtgaggcc gtgcagccgc acgtccacga acccggccga cgccagcaca cgacgcacgc
     1501 ggtcgggatc gctcagcgca aaggggcccg gtgcccccgg tggaggcacg ggtagatcac
     1561 gaccggcagc cagggcagtg cggaacgcgc ggagccactc gttctgctcg aacggctgcc
     1621 aggccaggag gacgaggcgt cccccgtcgc gcacggcacg cgcgatgttg gcgaacgccg
     1681 ccggagcatc gccgaagaac atcgccccgc tccggctgat ggcgatgtcg aaggaccggt
     1741 cggggaaggg atggttctgg gcatcggcct gctggaacgt cacgttcgcg acctgttccc
     1801 gctgcgccag ccggcgcgcg agctcgatca tgcgcgagga cagatccacc ccgagtgccg
     1861 acccggccga ggcgcatcgc gcggagtccc gggtcgtctg tccggcaccg cacccgatgt
     1921 cgagcacgtt ctcggttgca tcgattccgg cggccgcgag caaatgaccg tggtgagcgg
     1981 ccactccctc gtcgaagcgc tcggcgtggg cagcccagaa gtcacccgtg tcgccgtccc
     2041 aggcatgtag ctgctcgacg ttggacgggt cggcctgcac accggtggtg tccgcgagtc
     2101 tccgtgtcac gtccgcagtc tggaacaccg gcccgacgat cttcaagggc aggccctgcg
     2161 cgccgactgg tcggcggtta cgccccgcgg gacagcggag gaaccttcgc gctggtcatc
     2221 atcatcgtga tcccgtcgtg ctcccggcta accccgacgt cgtccatgca cgagtggatc
     2281 atgagcagac cctggccggg gaagctcgcc ggctcggggc tccacagctt gccccccgcc
     2341 accgcgatga ggatacgcct cccgccgtcc agctcggcgt actgccgcgc cgccacgcgc
     2401 acctcggccc cgatctccga cgggtggatc tgctcgatgg cgttcgacac cgcctcgttg
     2461 acggccatca cgacatcgtc gacctcctcc tccggccagc agagggcggc cagccaccgg
     2521 cggaagcgct ggcgcacgac cgacagtgac accggatccg ccatgaggac catggagatc
     2581 acggcatcta ggtcatcggt gtacgacatc agctgcgcgc ctgatcacgc cgtgatggcc
     2641 tcgtcgaggg agccgaacag cgagagcacc tcgtccagtc ccatcgtcct gatcgggcgg
     2701 atcaccgact gtttctcgtc gacgaccacc cgcagcgggg tgtgcatgcc acggccttcg
     2761 tcgtgcagtg cgaccagcag ccccatcccc gaggaggaca ggaagtcgac gtccgagagg
     2821 tcgaggacca ccggctgacc cgcggcctgg tgcagagcct cgctccccgc ctccatcagg
     2881 cggctaccgg tgctcaggtc cacttccccg tgcaccacga gcaccacgca cccgctccgg
     2941 tcgtcacggt tgatcgtcat cagctgctcg gccggcggca ctccgccgag agggccgttc
     3001 atgctggcga agcgtacaga tggtcagcca tggttcatcc ccatgtggtg tcgccacggt
     3061 gagcgagcgg cgttgagcgt cgcgcaggac gcgccgggcc agcgcaacga tgtccttcag
     3121 accgcggcgg tcgagcagcc gagccacgtc acgggccgcc gccagtgcga cctcgtcgcc
     3181 ctccgccgac tgctctcgtg ccatcgattc ctgtgtcata ggccccccgg ctgccatcgt
     3241 gacgacttcc tccgatcatg cccgtacccg gtgcccgacc ggcggcaaac ctccgcggac
     3301 cgtcgggcgg cagccgattc ccggcgccag cacggcaact gggacaatgg ccacgaagag
     3361 gtgggcccaa ggcccaccgg gagaggggaa cggtgacggc gacggacgag gcgctcgaca
     3421 tggcccgcgt ggcggtggcc gccgctgccg acaagaaggc gcaggacatc gtcgtgctcg
     3481 acgtgtccga tcagctggtg atcaccgact gcttcgtgat cgcctccgcg cccaacgagc
     3541 ggcaggtcca ggccatcgtc gacgaggtcg aggagaagct ccggcagcac ggggcgaagc
     3601 ccacgcgccg cgagggcacc cgggaagggc gctgggtgct gctggacttc gtcgacgtcg
     3661 tggtgcacgt ccagcacacc gaggagcgcg ggttctacgg gctcgaccgg ctgtggaagg
     3721 actgcccacg ggtcgcgttc ccggaggccg agaccgaggc tgttccggac gggtcggcgc
     3781 cggtgggcgt cgacggggac gggaccgggt gagcctgcag cggctggtcc tgctccggca
     3841 cggccagacc gactacaacg ttgcgggccg gatgcagggc catctcgact cggtgctcac
     3901 cgagacgggg ctggcccagg cggccggcgt cgcacccgag atcgcccgct tcgcccccga
     3961 ccggctgatc agctccgacc ttcggcgtgc ggtggacacc gccgacatcg tcggggccgc
     4021 ctgcagccag cccgtcaaac tcgacgcccg gctgcgcgag acgcacctcg gggagtggca
     4081 gggctgctcg gtggcggaga tcgagcaggc atggcccggc gcgatcgccg cgtggcgctc
     4141 cgatcccgcc tggacgccgc ccggtggcga gtcgcgcatc gacgtggtcc ggcgttcgca
     4201 accggtggtc gacgagttgg acgaggagtt cgcgtcggcg cccgacggca ccgtggtgct
     4261 cgtcgcgcac ggcgggctga tcgcgggcct ggtcagcgga ctgctcgcat tgcccacatc
     4321 ggcgtggccg tcgatcggcg gtatcggcaa ctgccactgg gcagcgcttg cccgccgcgc
     4381 cgaccacccg cgctggaggt tggtcggcta caacgtcggc acgtggtgaa tgacgcggcc
     4441 agtgcaggcc cgcacggcgt gctcggaccc gccgactcgt cggcgggggt gggcggagcc
     4501 gctgctgtcg gtcggcggta cggcgtgccg gacgtagctc aggtgggcgg gcccatcttg
     4561 ctggtactcg ccgactcgct cgccttccac ggcccagagc gggccgaacc cgccgacgag
     4621 cccaggctgt ggccgaacgt ggccgccacc gcgctcggcg ggcgcgcgga gctggtcgcc
     4681 gggatcgggt ggacggcccg gcatgcctgg cacgcactca cccacgatcc gcgggtgtgg
     4741 gcgctcatgc ccaccgtcga cgcgctggtg ctcggcgtgg gcgggatgga caccctcccc
     4801 tccccgctgc ccaccgcggt gcgcgagctg atcccggcgc tgcgacccga cggactgcgc
     4861 cgtgcggtgc gggcaggata cctgcgggcc cagcctccgc tcgcccgcac gttggcctcg
     4921 gtggtgcccg gtggcggccc ggttgcgctc ccgccgcggc tgacggtgcg ctacctggag
     4981 cggtgccggc gggcagttct cgcgctccgg ccggggatcc cggtcgtcgc ggtgctgccg
     5041 cccgtgcacc gcgccgccgt ctacgggtcg gtgcacgcgg gccgtgccgc ggccgagtac
     5101 gcgacccgcg cctgggcgcg cgggtgcggt gctgccctgc tcgacctcag ggcgctcgtc
     5161 ggcgagcacg tgctcggcgg ccatggcaac cctgacggca tgcactgggg atggcaggcg
     5221 cacgcggcgg tcggtgcggc gtgcgcggag ttgctgcacc ccagggtgga gcgctgacgg
     5281 gcgtccctgc catagggtgc gtcgtcgtgc ccgtcgccgt cgtgaccgac tccaccgcgt
     5341 acctgcccgc cggcgtggcg gaggagcgcg agatccgcgt ggtgccgctg gaggtccggc
     5401 tggggcagcg cgtgggtcgc gaggggtgcg acatcggcat caccgagctg tccgccgcgc
     5461 t
//
LOCUS       DASRSC010000185         3807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_63863,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000185 DASRSC010000000
VERSION     DASRSC010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63863"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1987)
                     /locus_tag="VFE39_09250"
                     /pseudo
     CDS             complement(158..1987)
                     /locus_tag="VFE39_09250"
                     /inference="COORDINATES: protein motif:HMM:NF020417.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
     gene            complement(1998..2579)
                     /locus_tag="VFE39_09255"
     CDS             complement(1998..2579)
                     /locus_tag="VFE39_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HET6258824.1"
                     /translation="MTTPAGERTVSLRETGPARRGTREDEVVIAVSPAFAGHFTKTIV
                     DVPHAEVLRQLLAGIEEQGVTARVIRVRDTADLAAIAHLGAKLSGSGIAVGVLSRGTT
                     MLHQKDLPRLSNLELFPQSPLLDAEVFRKIGSNAAQYAKGESPEPVPTRNDQMARPRW
                     QAKAALLHLKEFECIEADRGPIEVEPAFELSPA"
     gene            complement(2582..>3807)
                     /locus_tag="VFE39_09260"
     CDS             complement(2582..>3807)
                     /locus_tag="VFE39_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase large
                     subunit"
                     /protein_id="HET6258825.1"
                     /translation="IQVAADAAEGALRGFAELETTLGVLRYAPLVAIALQIGAQVGRG
                     GVLTQCALEEATELELGMRGITAYAETISIYGTESVFVDGDDTPWSKAFLASAYASRG
                     IKMRFTSGTGSEVQMGNAEGKSMLYLEIRCILMAKGAGVQGLQNGSISCIGVPGAMPG
                     GIRAVAAENLVASMVDLECASGNDQSFSHSAMRRTARAMPQLLPGTDFVCSGYSGVPN
                     LDNMFAGSNFDTDDYDDWNTIQRDLQVDGGLRHVREEEILQARNTAARALQALFAHLD
                     LPAVTDQEVEAATYANSSNDYPLRDVLADLKGAQSVMDRGLTGLDLVKGLEATGFHDV
                     AENLLTVLRQRVSGDLLQTSAILTPDFVPLSAINDANDYAGPGTGYRLQGERWEQLKK
                     LRHVTSATNPETEVG"
BASE COUNT          498 a         1397 c         1352 g          560 t
ORIGIN      
        1 gctggggcgt cgcgaccgtc ggtgtccccg acgggctcga cccgggtcgg cacgccctcc
       61 tcctctgcac ccgcgcatac ctcgtgcagc acggcggggg acgcgccggg gtgccggtag
      121 atgacgaccg acgggcgctc cacctcccgg ccgtcgctca cgtcgactcc tcggcgtacg
      181 cgtccaccag cccggacgcc accgcgttgc gcgggccttc gctgccgtgg atgttgccgg
      241 tgccgcagac gatgccgtgt ggggcgagcg cgtcggcgat catgtcgggg atctcgaagt
      301 cgagcgccga gccgccgagc agcaccacga agtccagcgc gcgcaggttg ccctgcggcg
      361 ccacctgccg cagtgcgcgc accgcgttca cgcagaacac ccgccgcttg gcctctcgcc
      421 gcacccggcg gacggcgtcg aggctgtgcc gcgtgggcac cgggaccagc ccgccgggag
      481 ccatcaccac gacgcgggcg aacacgtgcg gcggcagggg ctcgtcgacg aattccaccg
      541 tgccgtcctc gtgccggagg tggtagaagc tctacacctt ggccagcggg tgccgcttga
      601 cactctcggc gatctcccgg tcgtccagcg ccagttcgga ggcgatgagc ttcgtgacca
      661 gctctccggc ccccgccaca tgtacggccg tggtctcgcc ggcggcggtg agcagggcgg
      721 cgtcggtgga gccgccgccg aggtcgatca ccgcgacggg gcggtcgacc ccgggggtgg
      781 tgagcgcccc tcgcacagcc atcgcgccct ccaccccgcc gacccccgcg gagctcccga
      841 gctgggcggc gacccggtcg gcgacggcct gcatgcggct gcggtccgtg ctcaccatcg
      901 ccgcgagcgc gacggcgtgc tcgagcgcga cctcgccggc gagtccgccc cgcacctccg
      961 cgggcacgat ggtgtcgacg gccagcacgt cgcggatggc gatctcgctc gccggctgcc
     1021 cggtgacgtc ggtcatcgtg tcgcggacct gcgcgagcat gcccccgacg tgggtgccgg
     1081 gctccccgtg cacgtcgtcg aggggctggg cccgggcgag ggtggccatg atctcgtcgg
     1141 ccccggcatc gacctcgacc cgcagggtct tgcccgctcc gcgcagctcc aacacccccg
     1201 ccgggatcag ccggtcggtg acgtccccgg tcggggtccg gatcacgaca gcggaccggt
     1261 tgccggtgag cgccctcgcc accggcgaga cctgccgggt ctgggcgggg tcgagcgcga
     1321 agacggtcgc gaggccgtag gagttcgaca gggtgcggat cgtccgccct ggcggcgcca
     1381 cctcgacggc ggcgagcatc cccatcggga ccttctccac cgccgcgacc tcgtcgacca
     1441 cggggatcgg tttctcgagc cggttggtga cgaggaccgc gtcgtcaccg gccagcaccg
     1501 ccgcgaccac gtccactccg tcgcggaccg ccgcgttgag ggtggccgcc acgtcgtcga
     1561 agtcccaacc gtgcgggacg atgacgatca gcgacccctc ggcgccgcgc cggtcgccga
     1621 gcagctcccc gatcggcacc gtgacgccca cgcctaggcc ctcgccgccc ggcgtggccg
     1681 ggttgtgccc gatcatcgtg gactcggtga tgatcgtctc ggtgatcgtc tccatcgcca
     1741 gcccgctgat caccggcgtg gcctcgttga gcagcaccgc gtgcagccga tcggtcaggg
     1801 gcccgacgcg ggcgagcacg gcgcgcaccg cggccactgc gccgtcggcg ttctccgggg
     1861 tgcctttgac ccccgtggtc cgcatcaggg cggtgccgag gtaggtcacc gccccctcgg
     1921 gcccgacgcg cgccagacag gcctcggtgg tggtgttgcc gacgtcgacg ccgacgacca
     1981 gcctcatgtc cacctcgcta ggcggggctc agctcgaacg ccggctccac ctcgatgggg
     2041 ccgcggtcgg cctcgatgca ctcgaactcc ttgaggtgca gcagcgccgc cttggcctgc
     2101 cagcgcggcc gcgccatctg gtcgttgcgc gtcgggaccg gctcgggcga ctcgcccttg
     2161 gcgtactgcg cggcgttgga gccgatcttc cggaacacct cggcgtcgag cagcggcgac
     2221 tgcggaaaca gctcgaggtt ggagagccgc ggcaggtcct tctggtggag catcgtggtg
     2281 ccgcgggaca gcacgcccac ggcgatcccg gagcccgaga gcttcgcgcc gaggtgggcg
     2341 atcgccgcga ggtccgcggt gtcgcggacc cggatcaccc gggccgtgac gccctgctcc
     2401 tcgatgccgg ccagcagttg gcgcagcacc tcggcgtgcg ggacgtcgac gatcgtcttg
     2461 gtgaagtgcc cggcgaacgc cggggacacc gcgatcacga cctcgtcctc acgggtgccg
     2521 cgccgggcgg gtccggtctc gcgaaggctg acggtgcgct ctccggcagg agtcgtcatc
     2581 gtcagcccac ctcggtctca ggattcgtcg ccgatgtgac gtggcggagc ttcttgagct
     2641 gctcccagcg ctcgccctga agccggtagc cggtacccgg gcccgcgtag tcgttggcgt
     2701 cgttgatcgc ggacagcggg acgaagtcgg gcgtgaggat cgccgatgtc tgcagcaggt
     2761 cgccggacac ccgctgacgc agcacggtca gcaggttctc cgcgacgtcg tggaacccgg
     2821 tagcctccag ccccttgacc aggtccagcc cggtcaggcc gcggtccatc accgactgcg
     2881 cgcccttgag gtcggcgagc acgtcgcgca gcgggtagtc gttcgacgag ttggcgtagg
     2941 tggccgcctc cacctcctgg tcggtgaccg ccggcaggtc caggtgcgcg aacagcgcct
     3001 gcagcgcgcg cgccgccgtg ttgcgcgcct gcaggatctc ttcctcccgg acgtgccgca
     3061 ggccgccgtc gacctgcagg tcgcgctgga tggtgttcca gtcgtcgtag tcgtcggtgt
     3121 cgaagttgga cccggcgaac atgttgtcga ggttgggcac cccggagtag ccggagcaga
     3181 cgaagtcggt gcctggcagc agttggggca tcgcccgcgc ggtccggcgc atcgccgagt
     3241 gcgagaacga ctggtcgttg cccgaagcgc actcgaggtc gaccatgctc gcgacgagat
     3301 tctccgcggc caccgcgcgg ataccgcccg gcatcgcgcc cggcaccccg atgcagctga
     3361 tcgagccgtt ctgcaggccc tgcacgccgg cgcccttggc catgaggatg cagcggatct
     3421 ccaggtacag catggacttg ccctcggcgt tgcccatctg tacctcggaa ccggtgcccg
     3481 aggtgaaacg catcttgatc ccacgcgacg cgtaggcgga ggcgaggaat gccttggacc
     3541 acggcgtgtc gtcgccgtcg acgaacaccg actccgtgcc atagatcgag atcgtctcgg
     3601 cgtaggcggt gatgccacgc atgccgagct cgagctcggt ggcctcctcg agcgcgcact
     3661 gggtgagcac gccgccgcgc ccgacctgcg cgccgatctg cagcgcgatc gccaccagcg
     3721 gggcgtagcg caggacgccc agcgtggtct ccagctccgc gaacccgcgc agcgcgccct
     3781 ccgcggcatc cgcggcgacc tggatcg
//
LOCUS       DASRSC010000186        11064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5634896,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000186 DASRSC010000000
VERSION     DASRSC010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11064
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5634896"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /gene="galE"
                     /locus_tag="VFE39_09265"
     CDS             complement(<1..876)
                     /gene="galE"
                     /locus_tag="VFE39_09265"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET6258826.1"
                     /translation="MKLLVTGGAGYVGSVCAAHLVDSGHDVTVLDDLSTGHLDAVPEG
                     ARFIEAELSAAADDVLAEGFDGVLHFAARSLVGESVQRPELYWSGNVVTSFRLLEAMR
                     RHGTLRLVFSSTAATYGEPEQVPIPEDAPTRPTNPYGASKLAIDHAIRSYAIAHGLAA
                     MSLRYFNVAGAHGRFGERHTVETHLIPLVLQVASGAREVVQVFGDDWPTADGTCVRDY
                     IHVDDLAAAHLLALEHAQPGRHDIYNLGSGAGFSVREVIEACRAVTGHAIPTTIAPRR
                     SGDPAVLIASSEAATA"
     gene            complement(1024..1743)
                     /locus_tag="VFE39_09270"
     CDS             complement(1024..1743)
                     /locus_tag="VFE39_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007027698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HET6258827.1"
                     /translation="MNELIDTTEMYLRTIYELEEEGVVPLRARIAERLGQSGPTVSQT
                     VARMERDGLVVVAGDRHLELTDPGRARAVSVMRKHRLAERLLSDVIGLEWEFVHAEAC
                     RWEHVMSDAVERKLVRLLDNPTISPYGNPIPGLDELSQLPDAAPVGVSAPPTLEVGLQ
                     RLDEFARRGGGRVEVRRIAEHVQVDADLMAELKDAGIVPGRDVDVRAISRFGDAVPVA
                     ADGADASVAPLIAHAVLVRAR"
     gene            1849..2715
                     /locus_tag="VFE39_09275"
     CDS             1849..2715
                     /locus_tag="VFE39_09275"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET6258828.1"
                     /translation="MTPLIAPSDLAALLDAPRSSERPVVLDVRYRLGGPPGKADHDAA
                     HLPGAVYLDLDTELADPPGAGGRHPLPSHDRLQAALRRAGVHDGSRVVAHDDGNGTVA
                     ARAWWLLRWAGLPADRVAVLDGGFAAWVGAGLPVTDEPSRPEAGDVTVRPGGMPVLDA
                     DGAAALARRGVLLDARVGPRYRGETEPVDAKAGHIPGALNAPAVDLAGPDGRWPAPEV
                     VAERFSALGVDDGPGDAAAYCGSGVTAAALVLAVEHARLRPPARPIALYVGSWSSWSA
                     DPSRPVATGAAP"
     gene            2753..3922
                     /locus_tag="VFE39_09280"
     CDS             2753..3922
                     /locus_tag="VFE39_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HET6258829.1"
                     /translation="MTPRAAVVWTPEFLRYDLGDDHPLDPIRLDLTMRLASGLGVLEG
                     IEPLCPEQAPDEEVQRVHVPSYLSAVKSAPEAPFGVGHGLGTEDNPIFFGMHEASALV
                     CGGSLLGAREIAEGRTDRAVNIAGGLHHAMADRASGFCVYNDCAVAISWLLDHGFDRI
                     AYVDVDVHHGDGVQASFYRDPRVLTISMHQHPMTLWPGTGWSSEHGEGDGTGYAVNVP
                     LPPGTGDGGWLRAFHAVVPSLITAFRPQILVTQCGVDTHYEDPLANLGLSVDGHRAIY
                     RALRELAEHAAGGKWLAVGGGGYGLFRVVPRSWTHLVATVLDRDVEPNRPLPAGWVAH
                     AAGLTGQQLPTAMTDGRETGYEPWTEGADRVDTAVMETRRAVFPLHGLDPDDPRD"
     gene            3924..6689
                     /locus_tag="VFE39_09285"
     CDS             3924..6689
                     /locus_tag="VFE39_09285"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6258830.1"
                     /translation="MTPSPASAPPAGEQPTTATDSSDAGPGYPRHWEADVVASDGGIV
                     HLRPILPSDADALLRLHESLSERTRYLRFFGPYPRISPRDLERFTTVDHRSRVAFICL
                     LGHEIIAVGRYEGLPGDGGPGGTTGTAGPDSAEVAFVVRDSHQGRGLGSILLEHLAAA
                     ARENGLRRFEAEVLIENHQMVRVFREAGYQISREFAEGVLHLEFDIDPTERSLAVRDA
                     REQRAEARSVHNVLHPTSVAVIGASTDPAKIGHAVLANLLRGNFTGPVFPVNPEARSV
                     RGVRAYSSVTEIPDEVDLAVVAVPASGIDEVMDSCLEKGVKALVVVSAGFADAGEGGT
                     VAERRLVAEARVHGMRVIGPNALGVANTDPEVRLNATLAPDVPGRGRVGFFSQSGALG
                     IAILAAAKERGLGLSTFVSAGNRADLSGNDLLQYWQTDPATDVVLLYLETFGNPRKFA
                     RLARRLARTKPIVAVKSGRHSGSTTALAASSAPIDDDSVKALFEQAGVIRVETLSELF
                     DTALLLAYQPLPAGPRVAVVGNSTALGVLVSDALLDEGMELAGPPVDVGTAAAPEEFA
                     AAVAKAIDDGAGADGHGKADALVTVFVPPVAVPGATYARALREAVAGADKPVVAVFLA
                     AEGVPAELAVPREDGSPGPGSVPSYPSPERATAALARVSRYARWRARPVGEFVSPAGL
                     DTDRARELVLRFGAVERHELTDGQAVELLACFGIPVIEFRRVTGADAAVAAADELGYP
                     VALKAVGVHWRHRTDLVGVRLDVVTPAGVRQAHADIARLTGQDEVYVQRMAPKGVSCV
                     LDVVEDPSFGSLLSFGLSGMATELLGDRAFRVVPVSDQDAAALVRAPRAAPLLAGYRG
                     TEPVRLDALEDLVLRVGKIAEDLPEVRSLSLDPVLASAEGAFVTGARVVLGPPPTRHD
                     GGPRRLR"
     gene            complement(6717..8378)
                     /locus_tag="VFE39_09290"
     CDS             complement(6717..8378)
                     /locus_tag="VFE39_09290"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HET6258831.1"
                     /translation="MPEFRHVDVLPLGPDDTDYRRLDIPPGRVVETPSLPGRTFLEID
                     PATITALVREAVHDIQHMLRPSHLGQLRSIIDDPEASPNDRFVATDLLRNACVSAGGV
                     LPMCQDTGTAIVIGKRTETVLTGGNDEEAVSRGIFEAYDELNLRYSQMAPTSFWDETN
                     TGTNLPAQVELYSAPGQAPKYEFLVMAKGGGSANKTFLYQETKALLNPKKLATFLDEK
                     LRSLGTAACPPYHLAIVVGGMSAEYTLKVAKLASARYLDALPDVGSDAGHAFRDRDLE
                     QQVLELTREFGIGAQFGGKYFCHDVRVVRLPRHGASLPVGIAVSCSADRQARAKITAE
                     GVFLEQLERDPARFLPEVTESDDLSEEVVRVDLNRPMDEIRAQLSSLPVKTRVSLSGP
                     LVVARDIAHAKIAERLEAGESMPDYLRDHPVYYAGPAKTPEGYASGSFGPTTAGRMDS
                     YVEQFQAAGGSLVMLAKGNRSKQVTTSCATYGGFYLGSIGGPAARLAQDCIRKVDVLE
                     YPELGMEAVWKIEVEDFPAFVVVDDKGNDFFAGVSQPTLQVTFRR"
     gene            8570..10948
                     /locus_tag="VFE39_09295"
     CDS             8570..10948
                     /locus_tag="VFE39_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET6258832.1"
                     /translation="MAGSILGTSVRRVEDLELITGASTYVGNLALDGVLHLAFVRSPL
                     AHARIIGIDTSGAQASPGVVAVFTAADLGLPAHHGLMVVNPDLPRPPLATDRVRFVGE
                     PVAVVVGETKAAAVDAVELVEVDYDPLPAVVDPEAALDPGSEPQFPELGSNLAAGIRS
                     DTDPLDDAEVVVRARMVNQRLAVMPMEGNAIAVLPGPWGEEGAGDELTIWVSTQMPHA
                     FRAQTAGLFGLDQDRVRVIAPHVGGGFGGKAGLLAEHTATIGAARALGRPVTWVETRS
                     ENLVSMPHGRAQVGFYELGLTRDGVITGLRARVVADSGAYAGFGGALALGPTYLLSQG
                     VYRIPKIGFDAAVALTNTTPVGAFRGAGRPEATAYLERMMDVAAAELDLDPVEIRRRN
                     FIQPADFPYTTIPGAHYDIGDYDLPLREALRMVDYQKVREEQANRRESGDPVQLGIGV
                     SVYVEITAGGGGTEFGSVTVHADGSATISAGTSGHGQGHPTAFAMLASDRLGIPMDKI
                     RFVQSDTAVVPRGGGTGGSRSLQMGGNAVRAAAEDVLEQARRRAAAMLEAAVDDVQLT
                     ENGEFGVAGVPSVTLTWAQVAGSAEAEGAELAAALDFKQEGATFPFGAHVSVVEVDTD
                     TGRVRPLRHVAVDDCGRILNPLLVNGQQHGGLAQGIAQALYEEVVYDPEGQPLTGTLA
                     DYRMPSAADLITYEAANTETPTPMNPLGAKGIGESATVGATPAVQNAVVDALRSLGVR
                     HVDIPCTPERVWRAVQDARTGRPADPWREPPAVFATLPVRSASAGAVEGGEI"
BASE COUNT         1515 a         3961 c         3994 g         1594 t
ORIGIN      
        1 agccgtggcg gcctcgctgg aggcgatgag cacggccgga tcaccgctcc ggcgcggtgc
       61 aatggtggtc gggatcgcgt gaccggtcac ggctcggcag gcctcgatca cctcacgcac
      121 ggagaagccc gcgccgctgc cgaggttgta gatgtcgtgg cgtcccggct gggcgtgctc
      181 gagcgccagc aggtgggcgg cggccaggtc gtccacgtgg atgtagtcgc gcacgcacgt
      241 tccgtccgcg gtgggccagt cgtccccgaa cacctgcacg acctcacgcg cgcccgacgc
      301 cacctgcagc acgaggggga tcaggtgcgt ctccacggtg tgccgctcgc cgaaacgccc
      361 gtgcgcgccg gccacgttga agtagcgaag gctcatggcg gccaggccgt gagcgatggc
      421 gtacgacctg atcgcgtggt cgatggcgag cttgctcgcc ccgtaggggt tggtggggcg
      481 ggtgggcgcg tcctccggga tgggtacctg ctcgggctcc ccgtaggtgg ccgcggtcga
      541 ggagaacacc agccggagcg ttccgtgcct gcgcatggcc tcgagcagcc ggaaggaggt
      601 cacgacgttg cccgaccagt acagctccgg cctctgcacc gactcgccga ccagggaacg
      661 ggcggcgaaa tggagcaccc cgtcgaaacc ctcggccagc acgtcgtcgg cggcggcgga
      721 cagctcggcc tcgatgaagc gggcgccctc gggcaccgca tccaggtgcc cggtggacag
      781 gtcgtcgagc accgtcacgt cgtggcccga gtccacgaga tgcgcggcgc agacactgcc
      841 gacgtagccg gctccgccgg tgacgagcag tttcacgaag gtcctcctgg gacatcgtcg
      901 gtggcggggc accacgccga gcgagcgcgt gccggcaccg cgcgccgctc tgcacgggag
      961 cgcgtcggcg gcgcccaccg tctcacgacg ctcccgtcta ccggacgacg gggagcggtt
     1021 gggtcagcgc gcgcgcacca gcacggcatg cgcgatgagc ggtgccacag aggcgtcggc
     1081 cccgtccgcg gctacgggaa cggcgtcacc gaagcgggag atggcccgca cgtccacgtc
     1141 gcgtcccggc acgatgcccg catccttgag ctccgccatc aagtcggcgt ccacctgcac
     1201 gtgctcagcg atacggcgca cctccacgcg gcccccaccg cgccgggcga actcgtcgag
     1261 ccgctgcagc cccacctcga gcgtgggcgg ggccgagacg cccaccggcg cggcgtcggg
     1321 aagctgcgac agctcgtcga ggccggggat cgggttgccg tagggagaga tggtggggtt
     1381 gtcgagcagc cgcacgagct tgcgctccac ggcatcgctc atgacgtgct cccaccggca
     1441 ggcctcggcg tgcacgaact cccactcgag cccgatgaca tcggagagca gtcgctcggc
     1501 caggcggtgc ttgcgcatca ccgacaccgc tcgcgcacgg ccggggtcgg tgagctcgag
     1561 gtgccggtca ccggccacca ccaccaggcc gtcgcgctcc atccgggcca cggtctggct
     1621 caccgtcgga ccgctctgac cgaggcgttc ggcgatccgc gcgcgcagcg gtacgacacc
     1681 ctcctcctcg agttcgtaga tcgtccggag gtacatctcg gtggtatcga tgagctcatt
     1741 cacgcgctca gccctttcgt caagccccca tcctatccgg gcaacacctc actgaggccc
     1801 accatccttg gcagaatgcc tgggcatcat ggaggggtgg tcgcaccgat gactcccttg
     1861 atcgctccgt cggatctcgc cgcactgctg gacgctccgc gctcgtcgga gcggccggtc
     1921 gtgctggacg tgcgctatcg gctcggtggg ccgcccggca aagccgatca tgacgccgca
     1981 cacctccccg gcgccgtgta cctcgacctc gacaccgagc tggccgatcc acccggcgca
     2041 ggtggccggc acccgctgcc gtcgcacgac cggctgcagg ccgcactccg gcgagcgggt
     2101 gtgcacgacg ggtcccgggt ggtcgcacat gacgacggca acggcacggt ggccgcgcgc
     2161 gcgtggtggc tgctgcgctg ggcagggcta cctgccgacc gggtcgccgt gctcgacggc
     2221 ggcttcgccg cgtgggtcgg cgcgggcctt ccggtcaccg atgaaccgtc gcgccctgag
     2281 gccggtgacg tcactgtgcg accgggcggc atgccggttc tcgacgccga cggtgccgca
     2341 gcacttgctc gtcgcggcgt gttgctcgac gcgcgggtgg ggccgcgcta ccggggggag
     2401 acggaaccgg tcgacgccaa ggcagggcac atccccggag cgctcaacgc acccgccgtc
     2461 gacctcgccg gtcccgacgg gcggtggccg gcgcccgagg tggtggcgga gcggttctcg
     2521 gcactcgggg tcgacgacgg tccgggcgac gctgccgcct actgcggctc cggggtcacc
     2581 gccgcggcgc tggtgctcgc cgtcgagcat gcccggctgc ggccgcctgc gcgtccgatc
     2641 gcgttgtacg tcggatcctg gtccagctgg tcggccgacc cgtcgcggcc ggtggccaca
     2701 ggcgcggcgc cctgaggcgc gcgagacgtg tccatcccct acggtcgcgc ccatgactcc
     2761 gcgagccgcg gtggtgtgga cccccgagtt cctccgctac gacctcggcg acgaccaccc
     2821 gctcgacccg atccgccttg atctgacgat gcgcctcgca tcggggctcg gcgtgctcga
     2881 gggcatcgag ccgctgtgtc ccgagcaggc gccggacgag gaggtgcagc gggtgcacgt
     2941 gccgtcgtac ctctcggcgg tgaagtccgc gcccgaggcg ccgttcgggg tgggccatgg
     3001 gctcggcacc gaggacaacc cgatcttctt tgggatgcac gaggccagcg ctctggtgtg
     3061 cggtggctcg ctgctcgggg ccagggagat cgccgagggc cggaccgacc gggccgtcaa
     3121 catcgccggc gggctgcacc acgcgatggc cgaccgcgcg tccgggttct gtgtctacaa
     3181 cgactgcgcc gtcgcgatct cctggctgct cgaccacggg ttcgaccgga tcgcctacgt
     3241 cgacgtcgac gtccaccacg gcgacggcgt gcaggcatcg ttctaccgcg acccgcgggt
     3301 cctcaccatc tccatgcacc agcacccgat gacactgtgg cccggcacgg gctggtccag
     3361 cgagcacggc gaaggcgacg gcaccgggta cgcggtcaac gtgccgctgc cgccgggcac
     3421 tggcgacggc ggatggctgc gcgcgttcca cgcggtggtg ccgtcgctga tcacagcgtt
     3481 ccgcccgcag atcctcgtca cccagtgcgg ggtcgacacc cactacgagg acccgctggc
     3541 caacctcggg ctgtccgtcg acggccaccg cgcgatctac cgggctctgc gcgagctcgc
     3601 cgagcacgcc gcaggcggaa agtggctggc cgtcgggggc ggcggctacg gtctgttccg
     3661 ggttgtcccg cgctcctgga cgcacctcgt cgctaccgtg ctcgaccggg atgtcgagcc
     3721 gaaccggccg ctgcccgcag ggtgggtggc ccacgccgcc gggctcaccg gccagcagct
     3781 gccgacggcg atgaccgatg gccgcgagac cggatacgag ccgtggaccg agggtgccga
     3841 ccgcgtcgac accgccgtga tggagacaag gcgcgcggtg ttcccgctgc acggcctcga
     3901 tcccgacgac ccacgggact gacatgaccc cctcgcccgc ctcggcgccc cctgccggtg
     3961 agcagcccac caccgcaacg gactcctccg acgccggccc gggctacccg cgccactggg
     4021 aggccgatgt cgtggcctcc gacgggggga tcgtgcacct gcgcccgatc ctgcccagcg
     4081 atgccgacgc gctgctgcgg ttacacgaga gcctgtccga gcgcacgcgg tacctccggt
     4141 tcttcggccc ctatccccgg atctcgccgc gtgacctgga gcggttcacg accgtcgacc
     4201 atcgctcgcg agtggcgttc atctgcctgc tcggccacga gatcatcgcc gtcggtcgct
     4261 acgaggggct gcccggcgac ggcggcccag ggggcaccac cggaaccgca ggccccgact
     4321 cggccgaggt ggcgttcgtc gtccgcgact cgcaccaggg gcgcggcctc ggctcgatcc
     4381 tgctcgagca cctcgccgcg gctgcccggg agaacgggct gcgccggttc gaggccgagg
     4441 tgctcatcga gaaccaccag atggtgcggg tcttccggga ggccggctac cagatcagcc
     4501 gggagttcgc cgagggcgtg ctgcacctcg agttcgacat cgaccccacg gagcgttcgc
     4561 tggccgtccg tgacgcccgc gagcagcggg ccgaggcgcg cagcgtgcac aacgtcctgc
     4621 atcccacgtc ggtcgcggtg atcggcgcgt ccaccgatcc cgccaagatc gggcacgccg
     4681 ttctggccaa cctgctccgc ggcaacttca ccgggccggt gtttccggtc aaccccgagg
     4741 cccgctcggt ccgtggggtg cgtgcctaca gctcggtcac cgagatcccc gacgaggtcg
     4801 acctcgcggt cgtcgccgta ccggcgtccg ggatcgacga ggtcatggac tcctgcctgg
     4861 agaagggggt caaggcgctg gtggtggtca gcgcggggtt cgccgacgcc ggggagggcg
     4921 gcacggtcgc cgagcggcgg ctcgttgccg aggcgcgcgt ccacggaatg cgggtgatcg
     4981 gccccaacgc gctcggggtg gccaacaccg accccgaggt gcggctcaac gccaccctcg
     5041 cccccgatgt gccgggccgg ggccgggtgg gtttcttctc gcagtccggg gccctcggca
     5101 tcgcgatcct cgctgcggcc aaggagcggg ggctcggcct gtccacgttc gtctccgcag
     5161 gcaaccgcgc cgacctgtcg ggcaacgacc tgctgcagta ctggcagacc gacccggcca
     5221 ccgacgtggt gctgctgtac ctggagacgt tcggcaaccc ccgcaagttc gcccggctcg
     5281 cgcgccgcct cgcccggacg aagccgatcg tggcggtgaa gagcggtcgg cactcgggtt
     5341 cgacgactgc gctcgcggcc agctccgcgc cgatcgacga cgacagcgtc aaggccctgt
     5401 tcgagcaagc aggcgtcatc cgcgtcgaga cgctgtcgga gctgttcgac accgcgctcc
     5461 tgctggcgta ccagccgctg ccggccgggc cacgggtggc cgtcgtcggc aactcgaccg
     5521 cgctgggcgt gctcgtcagc gatgccttgc tcgatgaggg gatggagctc gccgggccgc
     5581 cggtcgacgt cggcacggcc gccgcgcccg aggagttcgc ggcggcggtg gcgaaggcga
     5641 tcgacgacgg tgcgggggct gacgggcacg ggaaggccga tgcgctcgtc accgtcttcg
     5701 tcccaccggt cgccgtaccc ggcgcgacct acgcgcgggc actgcgcgag gccgtcgcgg
     5761 gtgccgacaa gccggtcgtc gcggtcttcc tcgcggccga gggtgtgccg gccgagctgg
     5821 ccgtgccgcg cgaggacggc tcgccgggtc ccggctccgt gcccagctac ccgagcccgg
     5881 aacgggcgac ggccgcgctc gcccgggtca gccgctacgc gcgctggcgc gcgcgcccgg
     5941 tgggggagtt cgtcagcccg gccggcctcg acaccgaccg ggcccgggag ctcgtcctgc
     6001 gtttcggcgc ggtggaacgg cacgagctta ctgacggaca ggcggtcgag ctcctcgcct
     6061 gcttcgggat accggtcatc gagttccgcc gggtcacagg agctgacgcc gccgtcgctg
     6121 cggccgacga gctcggctac ccggtcgcgc tcaaggcggt cggcgtccac tggcgccacc
     6181 gcaccgacct cgtgggggtg cgactcgacg tcgtcactcc cgccggcgtg cgccaggccc
     6241 acgcggatat cgcgcggctc accggtcagg acgaggtcta cgtgcagcgg atggcaccca
     6301 agggggtgtc gtgcgtcctg gacgtcgtgg aagacccctc gttcggttcg ctcttgtcgt
     6361 tcgggctgtc gggaatggcc acagagctgc tgggtgaccg ggcgttccga gtggtcccgg
     6421 tgtccgacca ggacgcggcc gcgctggtcc gcgcgccgcg ggccgccccg ttgctggccg
     6481 gttaccgcgg caccgaaccg gtccggctgg acgccttgga ggacctggtg ctgcgcgtcg
     6541 gcaagatcgc cgaggacctg cccgaggtgc gttcgctcag cctcgacccg gtgctcgcct
     6601 cggccgaggg cgcgttcgtc acgggggcga gagtggtgct cgggcccccg cccacccggc
     6661 acgacggcgg cccgcgccgc ctgcggtgag ggccgacagg gcagccgtca gggccgctac
     6721 ctccggaacg tcacctgcag cgtgggctgg ctgaccccgg cgaagaagtc gttgcccttg
     6781 tcgtccacca cgacgaatgc cgggaagtcc tccacctcga tcttccagac cgcctccatg
     6841 ccgagctcgg gatactccag cacgtcgacc ttgcggatgc agtcctgtgc cagccgggcc
     6901 gcagggccgc cgatggagcc gaggtagaac ccgccgtagg tggcgcagga ggtggtcacc
     6961 tgtttcgacc ggttgccctt ggccagcatc accagtgacc cgcctgcggc ctggaactgc
     7021 tccacatagg agtccatccg gccggccgtg gtgggtccga aggacccgga tgcgtacccc
     7081 tcgggcgtct tcgcgggtcc tgcgtagtag acggggtggt cgcgcaggta gtccggcatg
     7141 ctctcgcctg cctccaggcg ctccgcgatc ttggcgtggg cgatgtcgcg ggccacgacg
     7201 agcggcccgc tgagcgagac acgcgtcttc accgggagcg aggacagctg cgcacgaatc
     7261 tcgtccatcg gtcggttgag gtcgacgcgc accacctcct cggacaagtc gtcggactcc
     7321 gtgacctcgg ggaggaagcg ggccgggtcg cgctccaact gctccaggaa caccccttcg
     7381 gcggtgatct tggcgcgggc ctggcgatcg gcagagcagg acaccgcgat gccgaccggc
     7441 agtgacgcgc cgtgccgggg cagccgcacc acccgcacgt cgtggcagaa gtacttgccg
     7501 ccgaactggg cgccgatgcc gaactcgcgg gtgagctcca ggacctgctg ctccaggtcg
     7561 cggtcgcgga acgcgtggcc ggcgtccgag ccgacatcgg ggagggcgtc gaggtaacgg
     7621 gccgaggcga gcttcgcgac cttcagggtg tactcggccg acatcccgcc gacgacgatc
     7681 gcgaggtggt acggcgggca ggcggccgtg ccgagcgagc gcagcttctc gtccaggaac
     7741 gtggcgagct tcttcgggtt cagcagcgcc ttggtctcct ggtagaggaa ggtcttgttg
     7801 gccgagccgc cgcccttggc catcaccagg aactcgtact tgggggcctg cccgggcgcg
     7861 ctgtacagct cgacctgcgc gggcaggttg gtgccggtgt tcgtctcgtc ccagaacgac
     7921 gtgggcgcca tctgcgagta gcgcaggttg agctcgtcgt aggcctcgaa gatgccgcgt
     7981 gatacggcct cctcgtcgtt gccgccggtc aggaccgtct cggtgcgctt gccgatgacg
     8041 atcgcggtgc cggtgtcctg gcacatcggc agcacgccac ccgccgagac gcacgcgttg
     8101 cgcagcaggt cggtggcgac gaaccggtcg ttcggtgacg cctcaggatc gtcgatgatc
     8161 gagcgcagct ggccgaggtg cgacggtcgc agcatgtgct ggatgtcgtg cacggcctcg
     8221 cggaccagtg cggtgatcgt cgccgggtcg atctcgagga aggtccggcc cggcagcgac
     8281 ggcgtctcga ccacgcgtcc cggagggatg tcgaggcggc ggtagtcggt gtcgtcggga
     8341 ccgaggggaa gcacgtccac gtggcggaac tcgggcaccc aacgaaccta acagcgccgc
     8401 ggccacgaca tggatcggga ggtcggaccg gcccccgacc agtccgaccc cccggggaat
     8461 gcgcgagccg acaagtcggc cgtgcaacgt cgcggtgaga ccttctcttg ctcggcgcgg
     8521 cacgtcaact tgtgagcgtt gcgaagcaac gttccgtagt gtcgacctta tggctggatc
     8581 gattctcggc acctccgttc gccgagtgga agacctcgaa ctcatcaccg gggcgagcac
     8641 ctacgtcggg aacctcgccc tcgacggcgt cctgcacctc gccttcgtcc gctccccact
     8701 ggcccacgcc cggatcatcg ggatcgacac atccggggca caggcatctc ccggcgtcgt
     8761 cgccgtcttc accgcggctg acctcggcct ccccgcgcac cacggcttga tggtcgtcaa
     8821 ccccgacctg ccgcgcccac cgctggccac cgaccgagtg cgcttcgtcg gcgagcccgt
     8881 cgcggtcgtg gtcggcgaga cgaaggccgc cgccgtcgac gcggtcgagc tcgtggaggt
     8941 cgactacgac ccgctgccgg ccgtggtcga cccggaggcc gccctggacc cgggctccga
     9001 gccgcagttc ccggagctcg gcagcaacct cgccgccggg atccgttccg acaccgatcc
     9061 gctcgacgac gccgaggtgg tcgtccgcgc tcgcatggtc aaccagcggc tcgccgtcat
     9121 gcccatggag ggcaacgcga tcgccgtcct cccgggcccg tggggtgagg agggagcggg
     9181 agacgagctg acgatctggg tgtccaccca gatgccgcac gccttccgcg cccagaccgc
     9241 cggcctcttc ggcctcgacc aggaccgcgt ccgcgtgatc gcgccgcatg tcggcggcgg
     9301 gttcggcggc aaggccggcc tgctcgccga gcacacggcg accatcgggg cggcgcgggc
     9361 actgggccgt ccggtcacct gggtggagac ccgctcggag aacctcgtct cgatgccgca
     9421 cggccgggcg caggtcggct tctacgagct gggcctcacc cgggacggcg tgatcaccgg
     9481 cctgcgggcc cgcgtggtgg ccgacagcgg cgcctacgcc ggcttcggcg gcgcacttgc
     9541 gttgggcccg acctacctgt tgagccaggg cgtgtaccgc atccccaaga tcgggttcga
     9601 cgccgccgtg gccctcacca acaccacgcc ggtcggcgcg ttccgcggtg cgggccgtcc
     9661 ggaggccacc gcctatctgg agcggatgat ggacgtcgcc gcggccgagc tggatctcga
     9721 tccggtggag atccgtcgac gcaacttcat ccagcctgcg gactttccgt acacgacgat
     9781 ccccggggcg cattacgaca tcggtgacta cgacctgccc ctgcgggagg cgctgcggat
     9841 ggtcgactac cagaaggtcc gggaggagca ggccaaccgc cgggagtcgg gcgacccggt
     9901 gcagctcggc atcggtgtga gcgtctacgt ggagatcacc gcgggcggcg gcggcaccga
     9961 gttcggttcg gtgaccgtgc acgccgacgg ctccgccacg atctccgcgg gtacgtcggg
    10021 acacgggcag ggccacccga cggcgttcgc catgctcgcc agcgaccgcc tcggcatccc
    10081 gatggacaag atccggttcg tgcagtccga cacagcggtg gtgccgcggg gtggcgggac
    10141 gggcggctcg aggtcgctgc agatgggtgg caacgccgtc cgcgcagcgg ccgaggacgt
    10201 gctggagcag gccaggcggc gcgccgcggc catgctggag gccgctgtcg acgacgtgca
    10261 gctcaccgag aacggcgagt tcggcgtggc cggcgtgccc tcggtgacgt tgacctgggc
    10321 gcaggtggcc ggctccgccg aggccgaggg agcggagctc gcggccgcgc tcgacttcaa
    10381 gcaggagggc gccacgttcc ccttcggtgc ccacgtgtcc gtcgtcgagg tcgacaccga
    10441 caccggccgg gtgaggccgc tgcgccacgt ggcggtggac gactgcggcc gcatcctcaa
    10501 cccgctgctc gtgaacggtc agcagcacgg cgggctcgcc cagggcatcg cgcaggcgct
    10561 gtacgaggaa gtcgtctacg acccggaggg ccagccgctc accgggacgc tcgccgacta
    10621 ccggatgccg agcgcggccg acctgatcac gtacgaggcc gccaacaccg agacgcccac
    10681 accgatgaac ccgctggggg ccaaggggat cggcgagtcc gccacggtcg gcgcgacgcc
    10741 agcggtgcag aacgcggtgg tcgacgccct gcgttcgctc ggggtgcgcc acgtcgacat
    10801 cccgtgcacc ccggaacggg tgtggcgggc cgtccaggac gcccgcaccg ggcgtcccgc
    10861 cgacccgtgg cgcgaaccgc ccgccgtctt cgcgacgctg ccggtgcgct cggcgtcagc
    10921 gggcgcggta gaaggcgggg agatctgagg caggcagctc ctcgccgtcc gggtaggtgg
    10981 atcacccccg ccgcgtgacc acgagagtgg tccgggccag agtgatcgtg acgacatttc
    11041 tcgacgtcga ggagttgccg gtga
//
LOCUS       DASRSC010000187         7671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4490616,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000187 DASRSC010000000
VERSION     DASRSC010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4490616"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1277
                     /locus_tag="VFE39_09300"
     CDS             150..1277
                     /locus_tag="VFE39_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HET6258833.1"
                     /translation="MRDLVEIGMGREARRSYDLSQVEIVPSRRTRSSQEVSTAWQLDA
                     YQFGIPLVTHPTDAVVSPASAVRIGQLGGLAVLNAEGLWARHADAEGALQRVLDAAAA
                     EDDPTAAVRLLQELHAAPIQPALLTEALKTVGEAGVTVAARVSPQRARELTPDLLAAG
                     LEILVVQGTIISAEHVSGGEPLNLKEFINSLDVPVVAGGVGDYRTAMHLMRTGAAGVI
                     VGYGESSATTTDDVLGIGVPMATAIVDAAAARRDYLDETGGRYVHVIADGGMAGSGDM
                     AKAIACGADALMLGEQLAEAAESPGQGLYWTSAAAHPSLPRSEVTGFAPSERDLQTLL
                     FGPASSPYGTVNLFGGLRRAMAKTGYSDLKEFQRVGLTVRG"
     gene            1472..3022
                     /gene="guaA"
                     /locus_tag="VFE39_09305"
     CDS             1472..3022
                     /gene="guaA"
                     /locus_tag="VFE39_09305"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET6258834.1"
                     /translation="MQPLTVLVVDYGAQYAQLIARRVREAQVYSEIVPGNTPVDEIVA
                     RKPAALILSGGPSSVYEPGAPSIDPELFTSGIPVLGLCYGFQAMALSLGGEVAPDGTR
                     EYGRTELTVCAAPGALHDGLPGRHPVWMSHGDSVVRAPDGFVVTASSERAAVAAFEDT
                     ERRLAGVQYHPEVGHSPYGQEVLRRFLHEIAGITPGWTTSSIIDDTIAAVRSQVGEGR
                     AICGLSGGVDSAGAAALVQRAIGDRLTCVFVDHGLLRAGEREQVERDFVAATGAKLHT
                     VDARKRFLDALDGVTDPEEKRKIIGREFIRVFEAATAEVAAEEHVGFLVQGTLYPDVV
                     ESGGGSGTATIKSHHNVGGLPDDIEFALVEPLRALFKDEVRRVGAELGLPETIVARQP
                     FPGPGLGIRIIGAVTQDRLETLRAADAIAREELTAAGLDRTIWQCPVVLLADVRSVGV
                     QGDGRTYGHPVVLRPVSSEDAMTADWTRLPYDVLERISTRITNEVSEVNRVVLDVTSK
                     PPGTIEWE"
     gene            3013..3936
                     /locus_tag="VFE39_09310"
     CDS             3013..3936
                     /locus_tag="VFE39_09310"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET6258835.1"
                     /translation="MGVAIPAERPGRLADALRTGRFAVTAEIGPPRGADVDEIRESAV
                     LLRDWVDAANITDNQGAQVRVSSFAGSIVALAAGLEPVMQMTCRDRNRIALQSDLLSA
                     GALGVPNLVLMTGDRPERGDHADATAVFDLSSSSLMTTARILRDEGRLLSGRAIDPPP
                     QYLLGAVDTGAGVPDRLEQKVADGAEFVQTQFVFDVDDFVVWIEKVRDLGLDHRCGIL
                     AGVGPVRSLRALEFIAGIPGVVIPPAFERRLRGVPAERVAAEGMAACAETVAALREVP
                     GVAGVHVMAVGYERGVPEILDAAGLEPTPRA"
     gene            complement(4084..4308)
                     /locus_tag="VFE39_09315"
     CDS             complement(4084..4308)
                     /locus_tag="VFE39_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258836.1"
                     /translation="MAEWDEKPATATRRRGPDVLTLLVGVLSLGMATAAFVGEVPTLA
                     GFDPRWLLAAGATLIGLMLLVGSLRGRGNS"
     gene            complement(4301..5524)
                     /locus_tag="VFE39_09320"
     CDS             complement(4301..5524)
                     /locus_tag="VFE39_09320"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET6258837.1"
                     /translation="MNGTDVQNTLREMWETRPARPRDDRQVAGVAAGIARRYDIDPTL
                     VRVGFVVAAFTGIGAALYIAAWFLLPEEPVDPAAPAEARSPRTIVVVGLAIAAVVSIG
                     TVFRGDSEIVLPTLVIAALLFLLHRSRGHRGSSGSRERAAAEAPTTVTPAGPSLLKEP
                     AGTGTPPAWDPLGAAPFAWDLPEPAPVGPQPEPPRRRLPVTPVTLGLALLAGGITAVI
                     MLVAGALSLANLPVLSGVVLAVIGAGLVIGAFLRAGRGLIPVALLLGALTWALLAAPL
                     DRWQGNGVGDLQAAPTTVAALQPSYQRTAGQITLDLRNLDLAVPAGGNSSPVRSTITL
                     GVGDVEVRVPQNADVSLTSTTGVGSVEFDGRSESGPGAHLAVTDDLGADGVRTGRPLI
                     LDIETGAGSVEVHRG"
     gene            5698..6981
                     /locus_tag="VFE39_09325"
     CDS             5698..6981
                     /locus_tag="VFE39_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET6258838.1"
                     /translation="MVPACSPPLVRRRSGRFVAGVAGGVADHLGVNVLWVRAAFILLA
                     GVHGAGVLAYGLLWIFVPQEHADVRRPLGRTERQQALGLAALGIGAGLAAAAVGNTVV
                     GWIVGPLGVVAIGAAVVWREADESQRRRWTEGARSGVTDLAGGGRTALLRVFAGAVFV
                     ASGIGVFLLGNLDLSQVQFGLLAVLATLLGVGVLTVPWWVRLMRELGEERRERIVETE
                     RAEIAAHLHDSVLQTLALIQRQSDQPREVLRLARGQERELRHWLYGPTGYGRSARASG
                     EPTLDAGLADAITTAAAEVEDTYAIDVRPVVVGDRPLDDGLRALVLAAREAMVNAAKH
                     SGVGEVSAYVEVEPTEVHVFVRDRGIGFDPDAVPDDRHGLADSVHGRMRRHGGAVRLR
                     STPGEGTEVHLSMPVDGPADTEPEAQAEAQKEATT"
     gene            6978..7670
                     /locus_tag="VFE39_09330"
     CDS             6978..7670
                     /locus_tag="VFE39_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6258839.1"
                     /translation="MTDTTDEAPGSEVTGQIRVFLVDDHGLFRAGVRAELERFVPDVT
                     VVGEAGSVDEAVAAIRHLRPDVVLLDVHMPDGGGAEVLRQLRPELPDVVFLALSVSDA
                     AEDVIAVIRAGARGYVTKTISGSELTEAVRRVKSGDAVFSPRLAGFVLDVFSDRPGAA
                     PPGDPELDLLTRRERDVLRLLARGYAYKEIAGELFISVKTVETHVSSVLRKTQLSNRY
                     ELSRWASDRRLV"
BASE COUNT         1014 a         2699 c         2892 g         1066 t
ORIGIN      
        1 ggagcgcaga cgatcgagca gctgcagcag gcgcagctgg tccggatcac cgccgcgggg
       61 ctcaaggaga gccacccgca cgacatcacg atgacggccg aggcgcccaa ctacgtggcc
      121 cgctagcggg atctctacgc tggtcacccg tgcgtgacct cgtggagatc gggatgggcc
      181 gggaggcccg tcgcagttac gacctgtcgc aggtcgagat cgtgccctcg cggcgtaccc
      241 gcagctcgca ggaggtctcg accgcctggc agctcgacgc gtaccagttc gggatcccgc
      301 tggtgaccca cccgaccgac gccgtcgtct caccggccag cgccgtgcgg atcgggcagc
      361 tgggcgggtt ggccgtgctc aatgccgagg gcctctgggc gcggcatgcc gatgccgagg
      421 gggcgctgca gcgggtgctc gacgccgccg cggccgagga cgacccgacg gctgcagtgc
      481 ggctcctgca ggagttgcac gccgcgccga tccagcccgc gctgctcacg gaggcgctca
      541 agaccgtggg cgaggcgggg gtcacggtgg ccgcgcgcgt gagcccgcag cgtgcccgcg
      601 agctcacgcc cgacctactc gctgccgggc tcgagatcct cgtcgtgcag ggcacgatca
      661 tctctgccga gcacgtctcg ggtggggagc cgttgaacct caaggagttc atcaactccc
      721 tcgacgtgcc ggtcgtcgcc ggcggggtgg gcgactaccg caccgcgatg cacctcatgc
      781 gcaccggcgc ggccggggtg atcgtcgggt acggggagtc gtcggcgacc accaccgacg
      841 acgtgctcgg tatcggggtg ccgatggcca ccgcgatcgt cgacgccgcc gcggcgcgcc
      901 gtgactacct cgacgagacg ggcggtcgct acgtccacgt gatcgccgac ggcggcatgg
      961 ccggctcggg tgacatggcg aaggccatcg cctgcggggc cgatgcgctg atgctgggcg
     1021 agcagctcgc cgaggcagcc gagtcgccgg ggcagggcct gtactggacc tcggcggcgg
     1081 cgcacccctc gctgccgcgt tcggaggtca ccggcttcgc gccgagcgag cgggatctgc
     1141 agaccctgct cttcggcccg gcgagcagcc cctacggcac cgtgaacctg ttcggtggcc
     1201 tccggcgggc gatggcgaag acgggctact cggacctgaa ggagttccag cgggtgggcc
     1261 tcaccgtccg cggctgacgc cgaacgcgca cccgggtcgc gaaccgtaca ccagctcccc
     1321 gcgaaggggg ccgcgtgcgg ctcgcggcga tcaccggggc cgctggcgtt gatgatcagc
     1381 gtgagccgcg cgtcagggcc ccgcggaggg cgctcgggta cggctcgcag ggatcaatac
     1441 tcggcgccag gggccgacta tcgtggtacg ggtgcaaccc ctcaccgtcc tcgtcgtcga
     1501 ctacggggcc caatacgccc agctgatcgc ccgcagggtg cgggaggcgc aggtgtactc
     1561 cgagatcgtg ccgggcaaca ccccggtcga cgagatcgtg gcgcgcaagc ccgccgcgct
     1621 gatcctgtcc ggcgggccgt cgagcgtgta cgagccgggt gcgccgagca tcgaccccga
     1681 gctgttcacc tccgggatcc cggtgctggg gctctgctat ggcttccagg cgatggcgct
     1741 ctcactgggt ggcgaggtgg caccggacgg cacccgcgag tacgggcgca ccgagctgac
     1801 ggtctgcgcc gcgccggggg cgctgcacga cggcctgccg ggccggcacc cggtgtggat
     1861 gagccacggc gactccgtgg tgcgtgcgcc ggatggtttc gtggtgacgg cgtcgtcgga
     1921 gcgggcggcg gtggcggcgt tcgaggacac cgagcggcgg ctcgcgggcg tccagtacca
     1981 ccccgaggtc gggcactcgc cgtacgggca ggaggtgctg cgccggttcc tgcacgagat
     2041 cgccggcatc accccgggct ggaccacttc ctcgatcatc gacgacacga tcgccgcggt
     2101 gcgctcgcag gtgggcgagg gaagggcgat ctgcgggctg tccggcggcg tcgactccgc
     2161 gggggcggcc gcgctcgtgc agcgggccat cggcgaccgg ctgacctgtg tgttcgtcga
     2221 tcacgggctg ctgcgggccg gcgagcggga gcaggtggag cgcgacttcg tggccgcgac
     2281 cggggccaag ctgcacaccg tcgatgcgcg gaagcggttc ctggacgcgc tcgacggggt
     2341 caccgacccc gaggagaagc gcaagatcat cggccgggag ttcatccggg tcttcgaggc
     2401 cgcgacggcg gaggtggcgg ccgaggagca tgtgggcttc ctcgtccagg gcaccctcta
     2461 ccccgacgtc gtcgagtccg gcggcgggtc cggcaccgcc acgatcaaga gccatcacaa
     2521 cgtgggcggg ttgcccgacg acatcgagtt cgcgctggtc gagccgctgc gggcgctgtt
     2581 caaggacgag gtacgccggg tgggtgccga gctcggcctg ccggagacga tcgtggcgcg
     2641 ccagccgttc cccggccccg gactgggcat ccggatcatc ggtgcggtca cccaggaccg
     2701 gctggagacg ttgcgtgccg ccgacgcgat cgcgcgcgag gagctcaccg ccgccgggtt
     2761 ggaccgcacg atctggcagt gtccggtggt gctgctcgcc gacgttcgca gcgtcggtgt
     2821 gcagggtgac ggccgcacct acggtcatcc ggtggtgctc cggccggtgt ccagcgagga
     2881 cgccatgacc gccgactgga cgcgcctgcc ctacgacgtg ctcgaacgca tctccacccg
     2941 gatcaccaac gaggtgtcgg aggtgaaccg cgtggtcctc gatgtcacca gcaagccccc
     3001 gggcacgatc gagtgggagt agcgatcccg gcggaacgac ccggccgcct cgccgacgcc
     3061 ctgcgcaccg gacggttcgc cgtcacggcc gagatcgggc cgccgcgcgg cgccgacgtg
     3121 gacgagatcc gcgagtccgc agtgctgctg cgtgactggg tggacgccgc gaacatcacc
     3181 gacaaccagg gtgcccaggt ccgggtgtcg agcttcgccg gcagcatcgt cgcgctcgct
     3241 gcgggtctgg agccggtcat gcagatgacc tgccgtgacc gcaaccggat cgcgctgcag
     3301 tcggacctgc tctcggcggg cgctctcggc gtccccaacc tcgtgctgat gaccggcgac
     3361 cggcccgaac gcggcgacca cgccgatgcg accgcggtgt tcgacctgag ctccagcagc
     3421 ctgatgacca ccgcgcgcat cctgcgggac gagggccggt tgctgtcggg gcgggccatc
     3481 gacccgccgc cgcagtacct gctcggcgcg gtcgacacgg gtgcgggtgt gcccgaccgg
     3541 ctcgagcaga aggtggccga cggagccgag ttcgtgcaga cccagttcgt gttcgacgtc
     3601 gacgacttcg tcgtctggat cgagaaggtg cgtgacctcg ggctcgacca ccggtgcggg
     3661 atccttgccg gtgtcgggcc cgtccggtcg ctgcgggcgc tcgagttcat cgccggaatt
     3721 cccggcgtgg tcatcccgcc cgccttcgag cggaggctgc gtggggtgcc ggccgagcgc
     3781 gtcgccgcgg agggcatggc cgcgtgcgcg gagactgtcg ccgcgttgcg agaggtcccc
     3841 ggcgtagccg gcgtgcacgt gatggccgtc ggctacgagc ggggcgtgcc ggagatcctc
     3901 gacgccgccg ggctggagcc gacccccagg gcctgagctg ccgcgccggc ggcggtgacg
     3961 acacgacgag ggcggcaccc gggtcgtcga cccctgatgc cgccccgcgt ggccgccgct
     4021 cccccgagcg cggcccccgg tcccgatggc tccccagtca ccggttccgg tcgttctccg
     4081 tgatcagctg ttgccgcgcc cccggaggct cccgaccaac agcatcaacc cgatcagcgt
     4141 cgccccggcg gcgagcagcc accgtgggtc gaagcctgcg agcgtcggca cctcgccgac
     4201 gaacgccgcc gtcgccatcc ccagcgagag cacccccacc aggagcgtca gcacgtcggg
     4261 gccacgccgc cgcgtcgcgg tcgccggctt ctcgtcccac tcagccacgg tgcacctcca
     4321 cgctccccgc gcccgtctcg atatcgagga tgagcggtct tcccgtccgg acgccatcgg
     4381 cgcccaggtc gtcggtcacc gcgaggtggg cccccggacc ggactccgag cggccgtcga
     4441 actccacact gcccaccccg gtcgtgctgg tcaggctgac gtcggcgttc tggggcaccc
     4501 gcacctcgac gtcgcccacg ccgagcgtga tggtggaccg caccgggctc gagttgccgc
     4561 ccgcgggcac cgcgaggtcg agattgcgca ggtcgagcgt gatctgcccc gccgtgcgct
     4621 ggtagctggg ctgcagtgcc gccacggttg tcggggccgc ctgcaggtcg ccgacgccgt
     4681 tgccctgcca gcggtcgagc ggcgcggcca ggagtgccca ggtcagtgcg ccgaggagca
     4741 gggcaaccgg gatcagaccg cgaccggccc ggaggaacgc gccgatcacc agcccggccc
     4801 cgatcacggc gagcaccacc ccggagagca ccggcaggtt ggccagcgag agcgcgccgg
     4861 ccacgagcat gatcacggcg gtgatgccac cggcgagcag agcgaggccc agcgtcaccg
     4921 gggtgacggg caggcgtcgc cgcggaggtt ccggctgcgg gccgacgggc gccggctcgg
     4981 ggaggtccca ggcgaacggg gcggctccga gtgggtccca tgccggaggg gtcccggtgc
     5041 cggccggctc cttgagaagc gagggccccg cgggcgtgac ggtggtcggg gcctccgcgg
     5101 cggcccgctc cctgcttccg ctcgagccac ggtggccccg gctgcggtgc agcaggaaca
     5161 gcagggccgc gatgaccagc gtgggcagca cgatctcgct gtcgccacgg aacacggtcc
     5221 cgatgctcac caccgctgcg atcgccaggc cgacgaccac gatcgtccgt ggtgaacgcg
     5281 cctccgcagg cgctgcaggg tcgacgggct cctctggcag gaggaaccac gcggcgatgt
     5341 agagcgccgc gccgatcccg gtgaacgccg ccacgacgaa cccgacgcgc accagcgtcg
     5401 ggtcgatgtc gtaccggcgc gcgatgcccg cggcgacccc cgccacctgc cggtcgtccc
     5461 gcggccgtgc gggccgtgtc tcccacatct cccgcagcgt gttctgcacg tctgtcccgt
     5521 tcatcgctct cctgcctcca tgggagacag cctgctcggt ctggcgcagt cgagacatag
     5581 gggatggccc ggagccgtcc cggaggtttc cccgatgtca ctcctccacg cacatgtgac
     5641 gatggtgacg tgcccacggt tcccgcggcg accccgccgc ccgcgccacc gccgacggtg
     5701 gtgcctgcat gctcgccgcc gctcgtccgc aggcgcagcg gccggttcgt cgcgggtgtg
     5761 gccgggggcg tcgcagacca cctcggcgtg aacgtgctct gggtgcgcgc ggcgttcatc
     5821 ctgctcgccg gcgtccatgg ggcgggggtt ctcgcctacg gactgctgtg gatcttcgtc
     5881 ccgcaggagc acgccgacgt ccggcggccg ctggggcgca cggagcgtca gcaggcgctg
     5941 ggactggccg cgctcggtat cggcgcgggg ctcgccgccg cggctgtggg caacacagtc
     6001 gtgggttgga tcgtgggccc gctcggcgtg gtggcgatcg gtgccgccgt cgtctggcgg
     6061 gaggccgacg agtcgcaacg ccgacgctgg acggagggtg cgcgttcggg ggtcaccgat
     6121 ttggccgggg gcgggcgcac cgcgctgctg cgggtgttcg cgggcgcggt cttcgtcgcc
     6181 agcgggatcg gcgtgttcct gctgggcaac ctggatctct cgcaggtgca gttcgggctg
     6241 ttggccgtgc tggcgacgct gctcggcgtc ggcgtgctga ccgtcccgtg gtgggtgcgt
     6301 ctgatgcgcg agctcggcga ggagcgccgc gagcggatcg tcgagaccga gcgcgcggag
     6361 atcgccgcac acctgcacga ctccgtgctc cagacactcg cgctcatcca gcgccagtcc
     6421 gaccagcccc gcgaggtgct gcggctggcc cgcggccagg agcgcgagct acggcactgg
     6481 ctgtacgggc ccaccggcta cggccgctct gctcgcgcgt ccggagagcc cacccttgac
     6541 gccgggctgg ccgacgcgat cacgacggcg gccgccgagg tggaggacac ctacgcgatc
     6601 gacgtccgcc ccgtcgtggt gggcgaccgg ccgctcgacg acgggctgcg ggccctcgtg
     6661 ctcgccgctc gcgaggcgat ggtcaacgca gccaagcatt ccggggtggg cgaggtgagc
     6721 gcgtacgtcg aggtggaacc gacggaggtg cacgtgttcg tccgcgaccg cggcatcggg
     6781 ttcgacccgg acgcggtgcc ggacgaccgg cacggcctcg ccgactcggt acacggcagg
     6841 atgaggcggc acggcggtgc ggtccggctg cggagcaccc cgggcgaggg caccgaggta
     6901 cacctgagca tgccggtgga cggaccggcc gacacggagc ccgaggccca ggccgaggct
     6961 cagaaggaag cgacgacatg accgacacca ccgacgaggc accgggtagc gaggtcaccg
     7021 ggcagattcg cgtcttcctc gtcgacgacc acggcctgtt ccgggccggg gtgcgcgccg
     7081 agctcgagcg gttcgttccg gacgtgacgg tggtgggcga ggccggctcc gtcgacgagg
     7141 cggttgctgc gatccggcac ctgcggcccg acgtggtgct gctcgacgtc catatgcccg
     7201 acgggggcgg cgccgaggtg ctgcgccagc tgcggccgga gctgccggac gtggtcttcc
     7261 tggccctgag cgtgtcggac gcggcggagg acgtcatcgc cgtgatccgt gcgggggcgc
     7321 gcggttacgt caccaagacg atctcgggga gcgagctgac ggaggccgtg cgccgggtga
     7381 agtccgggga cgcggtgttc tcgccgcggc tggccgggtt cgtgctcgac gtgttctccg
     7441 accggcccgg tgctgccccg cccggcgacc cggagctcga cctgctcacc cgccgggagc
     7501 gcgacgtgct gcggctgctc gcgcggggct acgcgtacaa ggaaatcgcc ggggagctgt
     7561 tcatctcggt gaagacggtg gagacgcacg tgtcgagcgt gctgcgcaag acgcagctgt
     7621 ccaaccgcta cgagctgtcg cggtgggcgt cggaccggcg gctcgtgtag c
//
LOCUS       DASRSC010000188         3838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_557994,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000188 DASRSC010000000
VERSION     DASRSC010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557994"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VFE39_09335"
     CDS             <1..519
                     /locus_tag="VFE39_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258840.1"
                     /translation="VRTMIVPRVGPPIARGRLRFLLPWFVFGAVAVAVVTTTTLWSVA
                     LRTSAAGRTTLDGAGIDMGAVPAAAPSLPSIAAPPPSAVATTTAAAVITTLRRPRPRV
                     LAPPAPALPSRAAAVTSAAPPPAPVPTTLPTAACVESDVDAATCASEVPSIASGRRTS
                     AVVAARDLVGGG"
     gene            531..1019
                     /locus_tag="VFE39_09340"
     CDS             531..1019
                     /locus_tag="VFE39_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyadenylate-specific 3'-exoribonuclease AS"
                     /protein_id="HET6258841.1"
                     /translation="MRFFYDCEFIEDGTTIDLVSIGVVGEDGREFYAVSTEFDPSRAG
                     AWVRANVLPKLPSPSDPAWRSRVRLRADLLEFLTSAPGEVELWAWIAAYDHVALCQLW
                     GAMPALPRPLPRFTRELRQRWEDTGRPPLPPPTSDAHDALADARLNAKRWEAIESAVA
                     AR"
     gene            complement(1053..1196)
                     /locus_tag="VFE39_09345"
     CDS             complement(1053..1196)
                     /locus_tag="VFE39_09345"
                     /inference="COORDINATES: protein motif:HMM:NF040511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPGG-motif small membrane protein"
                     /protein_id="HET6258842.1"
                     /translation="MATLLWIIAVVLVIAGVLAILRKQLVWGIVLIIVGLLVGPGGVS
                     IFV"
     gene            1384..2772
                     /locus_tag="VFE39_09350"
     CDS             1384..2772
                     /locus_tag="VFE39_09350"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HET6258843.1"
                     /translation="MNWTVDAPVEVLPELPPLPPELRARLDDALARPAAQQPDWPDAA
                     HVAHVRTVLESVPPVTLPPEVDRLRERLAQVARGEAFLLQGGDCAETFVDNTEPHLRA
                     TIRTLLQMAIVLTYGASLPVVKVGRIAGQYAKPRSAPVDALGLPSYRGDIVNSLVAHP
                     AARVPDPSRMVRAYANASAAMNLVRALTATGMADLTMVHDWNKDFVRTSPAGERFENI
                     AAEIERALRFMDACGVDDHNLHSVEFYASHEALLIDYERAMLRLDASRPEPRLYDLSA
                     HFVWIGERTRQLDGAHVAFAELLANPIGLKIGPTTTPELAVEYVERLDPDGTPGRLTL
                     VSRMGNGKVRDTLPPIVEKVEASGHKVIWQCDPMHGNTVESTTGYKTRHFDRIVDEVQ
                     GFFEVHRALGTHPGGIHVEVTGEDVTECLGGAQEISDADLAGRYETACDPRLNTQQSL
                     ELAFLVAEMLRG"
     gene            complement(2877..>3838)
                     /locus_tag="VFE39_09355"
     CDS             complement(2877..>3838)
                     /locus_tag="VFE39_09355"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HET6258844.1"
                     /translation="RVAARLEVPRVPPPVPPALPVEEQETVPSGPRRVLAGAHGTGTY
                     PAPHPVPGGGRTGTRMMPRPDAEYLAAPDGPPPHLRARRRSRRIFGVWVAIVLVLCLL
                     VGATAWWLGTGRWTAMPPLVGLEQTTAQQLLGQADLVASVTTAHDDGQPAGVVSATDP
                     PPDARLLRGSTVQLTISAGRPTVPAVAPGTAVAVAEQAVRDAGLTPVRTTDADQFSGT
                     VPTGAVVGTDPASGTALPLGAPVTLVLSRGPEPPPDDRVEVPFVIGKRFGDAADKLGD
                     AGLDAEEDSRGFPFGRDNGRVIEQDPSAGSLVEPGTTVTLHTL"
BASE COUNT          526 a         1413 c         1375 g          524 t
ORIGIN      
        1 gtccgcacca tgatcgttcc gcgggtcggg ccgcccatcg cccgcggccg cctgcggttc
       61 ctgctcccgt ggttcgtgtt cggcgcggtc gccgtcgcgg tggtcacgac cacgaccttg
      121 tggtcggtgg cgctgcgcac ctcggcggcg ggtcgcacca cattggacgg cgccgggatc
      181 gacatgggcg ccgtgcccgc ggcggccccc agcctgccgt cgatcgccgc tccgccgcca
      241 tccgccgtcg cgaccacgac cgccgccgcg gtcatcacca cgctccgcag gccgcgaccc
      301 cgcgtgctcg cgccgcctgc tccggcgctc cccagcagag ccgccgctgt caccagcgcg
      361 gcgcctccac cggccccggt tccgacgacc cttccgacgg ccgcgtgcgt tgagtcggac
      421 gtcgatgccg ccacctgtgc gagcgaggtc ccctcgatcg cgtccggtcg gagaacgtcg
      481 gccgtggtgg ccgcgcgcga cctcgtcggc gggggatagc ctcgggagtc gtgcgcttct
      541 tctacgactg cgagttcatc gaggacggga cgacgatcga cctggtgtcg atcggcgtgg
      601 tcggggagga cggccgcgag ttctatgccg tctcgacgga gttcgatccg tcgcgggccg
      661 gcgcctgggt gcgtgccaac gtgctgccca agctcccctc accgtccgac ccggcttggc
      721 gctcacgcgt gcggctgcgc gcggatctgc tggagttcct gacctccgcc ccgggggagg
      781 tcgagctgtg ggcgtggatc gccgcgtacg accacgttgc gctctgccag ctgtgggggg
      841 cgatgccggc gctgccgcga ccgctgcccc ggttcacccg tgagctccgg cagcgctggg
      901 aggacacggg acgtcccccg ctgccaccgc cgacctcgga cgcccacgac gcgctcgccg
      961 acgcccggct gaacgcgaag cgctgggagg ccatcgagtc ggctgtggcc gcccgctgac
     1021 ctgcagtggg cggccaccga tcggatgcgg ggtcagacga agatgctgac cccgccgggc
     1081 ccgacgagca ggccgacgat gatgagcacg atgccccaga cgagctgctt gcgcaggatc
     1141 gcgagcacgc cggcaatgac gagtacgacg gcgatgatcc acagtagggt tgccatgcac
     1201 gggagtgccc tgatcagtcc ctcgtggaat cgggccgttc gggtgacgcg gcgaccgtac
     1261 ggtccgcacg agagtcgtcc gggagggctc cgaacggtgt aatgcgcctc gatggcggct
     1321 gcttttcgcg cagtgacgga gcccggccac ggaccgcgcc tcacacggcc tactctgaaa
     1381 actgtgaact ggaccgtcga cgcaccggtc gaagtgctgc ccgagctgcc ccccttgcct
     1441 cccgaactgc gcgcccgcct ggacgacgcg ctggctcgtc ccgccgccca gcagcccgac
     1501 tggcccgacg ccgcacacgt cgcacacgtt cgcacggtgc tcgagagcgt ccctccggtc
     1561 accctgccgc cggaggtcga ccggctccgg gagcggctgg cccaggtcgc ccgcggggag
     1621 gccttcctcc tccagggtgg cgactgcgcc gagaccttcg tcgacaacac cgagccgcac
     1681 ctccgcgcca cgatccgcac gctgctgcag atggcgatcg tcctcaccta tggcgcgtcg
     1741 ctgccggtgg tgaaggtggg gcggatcgcc gggcagtatg cgaagccccg cagcgcgccc
     1801 gtcgatgcgc tgggcctgcc ctcctaccgc ggcgacatcg tcaactcgct ggtcgcgcac
     1861 ccggccgccc gggtgcccga cccatcccgc atggtgcgtg cctacgccaa cgccagcgcg
     1921 gccatgaacc tggtgcgtgc cctgacggcc accggcatgg ccgacctcac gatggtgcac
     1981 gactggaaca aggacttcgt gcgcacgtcg ccggcgggcg agcggttcga gaacatcgcc
     2041 gcggagatcg agcgggccct gcggttcatg gacgcgtgcg gcgtggacga ccacaacctg
     2101 cacagcgtcg agttctacgc gagccacgag gcactgctga tcgactacga gcgggcgatg
     2161 ctccggctgg acgcaagccg gccggagccg cggctctacg acctctcggc gcacttcgtg
     2221 tggatcgggg agcgcacccg ccaactcgac ggcgcccacg tggccttcgc cgagctgctg
     2281 gccaacccga tcgggctcaa gatcggcccg accaccacgc ctgagctcgc ggtcgagtac
     2341 gtcgagcggc tcgacccgga tggcacgccg gggcggctca ccctggtcag ccggatgggc
     2401 aacggcaagg tccgcgacac gctgccgccg atcgtcgaga aggtggaggc gtccgggcac
     2461 aaggtcatct ggcagtgcga cccgatgcac ggcaacaccg tcgagtccac caccggctac
     2521 aagacgcgcc acttcgaccg gatcgtcgac gaggtacagg gcttcttcga ggtgcacagg
     2581 gcgctgggca cccatcccgg gggcatccac gtcgaggtca caggcgagga cgtcacggag
     2641 tgcctgggtg gggcccagga gatctcggac gccgacctcg cgggtcggta cgagaccgcc
     2701 tgcgaccccc gcctgaacac acagcagtcc ttggagctcg cgttcctcgt ggcggagatg
     2761 ctccgcggct gacgccccac ccagcccagc ccgggagtcg ggagctggat tcggccgact
     2821 cgggggcggg gaacgggcga ctcgcgggct ggattcgggc gactcgcggg gtgtggtcag
     2881 agggtgtgga gggtgaccgt ggtgccgggc tcgacgagcg acccggcgct cggatcctgc
     2941 tcgatcacgc ggccgttgtc gcgaccgaag ggaaacccgc gagagtcctc ctccgcgtcc
     3001 agcccggcat cgcccagctt gtcggcagcg tcgccgaacc gcttgccgat cacgaaaggc
     3061 acctccacgc gatcatccgg tgggggctcg gggccccggc tgagcaccag ggtgacgggt
     3121 gcgcccagcg ggagcgcggt gccgcttgcc ggatcagtgc cgacgacggc gcccgtgggc
     3181 accgtgccgc tgaactgatc ggcatccgtg gtgcgcaccg gggtgaggcc cgcgtcccgc
     3241 acggcctgct ccgctaccgc cacggcagtc cccggggcca ccgccggcac cgtcggccgg
     3301 ccggctgaga tcgtgagctg cacggtgctg ccgcgcaaca gccgggcgtc gggtggggga
     3361 tcggtggccg acacgacacc ggcaggctgc ccgtcgtcgt gcgccgtggt gacgctcgcg
     3421 acgaggtcgg cctggccgag caactgctgc gccgtggtct gctcgagccc gaccaggggc
     3481 ggcatcgccg tccaccgtcc ggtgccgagc caccaggccg tcgcccctac caacaggcag
     3541 agcacgagaa cgatcgcgac ccagacgccg aagatccgtc ggctgcgccg ccgggcgcgc
     3601 aggtgcggcg gtggcccgtc cggcgccgcg agatactccg cgtcgggccg cggcatcatc
     3661 cgcgtgccgg tgcggccccc gccgggcacg ggatgcgggg cggggtacgt gccggtgcca
     3721 tgcgctccgg cgagcacgcg ccgcgggccg gagggcaccg tttcctgctc ctccaccggg
     3781 agtgccggcg gcaccggcgg cggcacgcgc gggacctcga gccgcgccgc cacccggt
//
LOCUS       DASRSC010000189         3773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_885996,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000189 DASRSC010000000
VERSION     DASRSC010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885996"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VFE39_09360"
     CDS             complement(<1..315)
                     /locus_tag="VFE39_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017988127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6258845.1"
                     /translation="MGRSVLVTGGHRGIGLAIAKAFAAQGDQVAVTHGSPVEGLPGEL
                     LPVQCDVTDTAAVDAAFTEIEDKHGPVQVLVSNAGITDDGLLMRMTEESFTRVVAPGF
                     VDT"
     gene            complement(479..1465)
                     /locus_tag="VFE39_09365"
     CDS             complement(479..1465)
                     /locus_tag="VFE39_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6258846.1"
                     /translation="MFSHPLWLLLLVVVAALAAGYVLLLRRRRRDTVRFTNLELLDRV
                     APKRPGWHRHLPAAALIGALAVLTVALAGPMAEARVPRNRATVVLVIDVSLSMQATDV
                     EPSRLAAAQVAAKAFADQLTPGINLGLVSFAGTAAVLVSPTVDREPIKRAVDGLKLSE
                     STATGEAIFAAMQSVETFSQAIAGAAADGPPPARIVLMSDGKQTVPGGVMPEEEPRGS
                     FTAARAAATAGIPVSTISFGTDYGSIEINPGERTPVAVDDASMRQIADLSGGQFFTAA
                     SEDELRQVYAELGEQIGYEVRRVDTSRPWLAGGALLLVAGVGSGLALGRRFP"
     gene            complement(1466..2404)
                     /locus_tag="VFE39_09370"
     CDS             complement(1466..2404)
                     /locus_tag="VFE39_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HET6258847.1"
                     /translation="MTAAARRPPIAPPSLRDGRIEAALRTLELAVRGRLDGLLQGNHL
                     GLVPGPGSELGEARTYQPGDDVRRMDWAVTARTTEPHVRETVADRELETWVVLDLSPS
                     LDFGTASCEKRDLAIATLAAVTHLTRGGGNRIGALVATGERTIRIPARGGIVHARGLL
                     RRVALTPRAPEGTRGDLTTAIEQLRRPPRRRGLAVVISDFLGEPDWERSLRALSNRHD
                     LLAVEVLDPRELELPDVGTVVLADPETGRQREVVTSPLLCREFAAAAAQHRDRVATAL
                     RRSGAAHLTLRTDSDWIADIVRFVLARKRAWSGGGR"
     gene            complement(2401..3450)
                     /locus_tag="VFE39_09375"
     CDS             complement(2401..3450)
                     /locus_tag="VFE39_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6258848.1"
                     /translation="MSVQESVPSPAHEGERLERVVFEIKRVIVGQDRLVERMLVGLLA
                     KGHLLLEGVPGVAKTLAVETVAKVVGGSFARLQFTPDLVPADILGTRIYRQGREEFDV
                     ELGPVVANFVLADEINRAPAKVQSAMLEVMAERHLSIGGRTFPMPDPFLVLATQNPIE
                     NEGVYPLPEAQRDRFLFKIIVEYPGVEEEREIVYRMGAEPPVAHQVIDPAELLRLQTV
                     ASQVFVHHALVDYVVRLVVATRTPAEHGLSDIAGWVSYGASPRATLGIVAAARALALV
                     RGRDYVLPQDVLDVAPDVLRHRLVLSYDAVADGVPMDHIISRVLATVPLPQVTARPQH
                     EPAAPVPYTSASSST"
BASE COUNT          553 a         1381 c         1337 g          502 t
ORIGIN      
        1 ggtgtcgacg aacccgggag ccacgacgcg cgtgaacgac tcctcggtca tccgcatgag
       61 cagcccgtcg tcagtgattc ccgcattcga gaccagcacc tgcaccgggc cgtgcttgtc
      121 ctcgatctca gtgaacgcgg cgtcgacggc cgcggtgtcg gtgacgtcgc actgcacggg
      181 cagcagttcg cccggcaggc cctcgacggg gctgccgtgt gtcaccgcca cctggtcgcc
      241 ctgcgccgcg aacgccttgg cgatcgccag cccgataccg cggtggcctc cggtcaccag
      301 cacgctgcgt cccacggggc ctaccctatg gtccggcgac ccaccggcgg aaaccgggcg
      361 actcgcgcgc ggattcgggc gactcgcgcg gcaccactcc gcgagtcgcc cgttctccgc
      421 ccccgagtcg cccgaatccc gccccgagtc gcgcacctgg cgcgcgcgag tcgcccgtct
      481 acgggaagcg gcgccccagg gccaggccgc tgcccacccc cgcgaccagc agcaatgcgc
      541 ctccggcgag ccatggtctg ctcgtgtcga cgcggcgtac ctcgtagccg atctgctcac
      601 cgagctcggc gtacacctgt cgcagctcgt cctcgctggc cgcggtgaag aactggccac
      661 ccgagaggtc ggcgatctgg cgcatcgacg cgtcgtccac cgccaccggg gtgcgctcgc
      721 cagggttgat ctcgatggag ccgtagtcgg tgccaaagga gatcgtggag acggggatgc
      781 cggccgtcgc ggccgcgcgc gccgccgtga acgagccccg gggctcctcc tccggcatca
      841 cgcccccggg caccgtttgc ttcccgtcgc tcatcagcac gatccgggcg ggcggcggac
      901 cgtccgcggc agcaccggcg atggcctgcg agaacgtctc gaccgactgc atcgccgcga
      961 agatcgcctc gccggtggcg gtcgactccg acagcttcaa cccatccacc gcacgtttga
     1021 tcggctcgcg gtcgaccgtc ggtgagacga ggacggccgc cgtacccgcg aacgacacca
     1081 gcccgaggtt gatgccgggc gtcaactggt cggcgaacgc cttggccgcg acctgggcgg
     1141 ccgccaacct gctcggctcg acgtcggtgg cctgcatcga caacgacacg tcgatcacca
     1201 gcaccaccgt ggcccggttg cgcggaaccc gcgcctccgc catcgggccc gccagggcga
     1261 cggtgagcac ggccagggcg ccgatcagcg ccgcggccgg gagatgccgg tgccagcccg
     1321 ggcgcttcgg ggccacccgg tcgagcagct ccaggttcgt gaaccggacc gtgtcgcggc
     1381 gacgcctgcg cagcagcagg acgtacccgg cggcgagcgc cgctaccacg accagcagca
     1441 gcagccacaa ggggtgggag aacatctacc gcccaccccc cgaccaagcg cgcttgcggg
     1501 ccagtacgaa ccgcacgatg tcggcgatcc agtccgagtc ggtgcgcagc gtcaggtgcg
     1561 cggcaccgct gcggcgcagc gccgtggcca cccggtcgcg gtgctgcgcg gctgcggcag
     1621 cgaactcccg gcacaggagc gggctggtga ccacctcccg ctgcctgccg gtctcggggt
     1681 cggcgagcac cacggtgccg acgtcgggca actccagctc acgcggatcg agcacctcga
     1741 cggccagcag gtcgtggcgg ttcgacaacg cgcgcagcga gcgttcccag tcgggttcgc
     1801 cgagaaagtc ggagatcacg acggcgaggc cgcgacgccg gggcggccgg cgcagctgtt
     1861 cgatcgcagt cgtgagatcg ccgcgagtgc cttcgggggc gcgcggcgtc aacgcgacgc
     1921 gacgcagcag cccgcgggcg tggacgatgc cgccgcgagc cgggatgcgg atcgttcgtt
     1981 cccccgttgc caccagggcg ccgatccggt tgcccccgcc gcgggtgagg tgggtgaccg
     2041 cggcgagcgt cgcgatcgcg aggtcgcgct tctcgcacga ggctgtgccg aagtccaggc
     2101 tgggcgacag gtcgagcacg acccacgtct ccaactcacg atcggccacg gtctcccgga
     2161 cgtgtggctc ggtggtacgg gcggtgacgg cccagtccat gcgccggacg tcgtcgcccg
     2221 gctggtaggt gcgtgcctcc ccgagctcgc tgccgggccc ggggaccagg ccgaggtggt
     2281 tgccctggag caacccgtcg aggcgccccc gaacggccag ctccagcgtc cgcagcgcgg
     2341 cctcgatccg cccgtcccgg agcgagggtg gcgcaatggg gggccgtcgg gccgcagccg
     2401 tcacgtcgag ctgctcgccg acgtgtacgg caccggggcg gccggctcgt gctggggacg
     2461 ggcggtgacc tgcggcagcg gaacggtggc gagcacccgg gagatgatgt ggtccatcgg
     2521 cacgccgtcg gcgaccgcgt cgtaggacag cacgagccgg tgccgcagca catcgggcgc
     2581 gacgtccagg acgtcctgcg ggagcacgta gtcgcggccg cgcaccagcg cgagcgcgcg
     2641 agcggcggcc acgatgccca gggtggcgcg cggtgacgcg ccgtaggaca cccagcctgc
     2701 gatgtcgctg agcccgtgct cggcgggggt acgggtggcg acgaccagcc gcacgacgta
     2761 gtcgaccagg gcgtggtgca cgaacacctg cgaggccacc gtctgcagcc gcaggagctc
     2821 ggcagggtcg atcacctggt gcgcgacggg cggctcggca cccatccggt agacgatctc
     2881 ccgctcctcc tcgacgccgg gatactcgac gatgatcttg aagaggaacc ggtcgcgctg
     2941 cgcctccggt agcgggtaga cgccctcgtt ctcgatcggg ttctgggtgg ccagcacgag
     3001 gaacgggtcg ggcatcggga acgttctgcc gccgatcgac aggtgccgct ccgccatcac
     3061 ctcgagcatg gccgactgca ccttcgccgg ggcgcggttg atctcgtcgg cgagcacgaa
     3121 gttggccacg accggcccga gctcgacgtc gaactcctcg cgaccctgcc ggtatatacg
     3181 ggtgccgagg atgtcggcag gcacgaggtc gggagtgaac tgcaggcggg cgaagctacc
     3241 gccgacgacc ttcgcaacgg tctcgaccgc gagcgtcttg gcgacgccgg gcacgccctc
     3301 gagcagcagg tggcccttgg ccagcaggcc gacgagcatc cgctcgacga gccggtcctg
     3361 accgacgatc acccgcttga tctcgaagac gacgcgctcg agccgctcac cctcatgggc
     3421 cgggctgggg acggactcct ggacgctcac gctgccatcc cacctcacgg caggtgagac
     3481 gccggtgaac ggaccgcacc gctccccccg gcgctgccgt cggctcggct tgaccgccgg
     3541 cttgtcatgg cggttcgacg gtcgtcgaat cgttgtcgaa acgctggcgt caatgtttca
     3601 ttgacagcgg gtgggtggac ggcgaagggc agccggtccc gatctcaccc cggcactcga
     3661 ccgcaccgac ctgctgccgg cggtggcggc cgaccggcac gaccacccat gtcgcggcac
     3721 ccctgatcga tccgcggccg cgctcccgga tcaccagcca ccgcgctcag agc
//
LOCUS       DASRSC010000190         6342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2854131,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000190 DASRSC010000000
VERSION     DASRSC010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2854131"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VFE39_09380"
     CDS             complement(<1..851)
                     /locus_tag="VFE39_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6258849.1"
                     /translation="MNRSAEAFYAAREFLVAHREDYETAYRDFRWPKIDEFNWAIDHF
                     DAVAADPERGAARALWIVEQDGSEGYWTFAELSTRSNQVANWLREHGVARGDRVILML
                     GNQVELWETLLAAMKLGAVVIPATTLLTASDLRDRLDRGGAKHAVVGGADTAKFDGVE
                     GDYTRIAVRGAPQGWLDYAGAAAAPADFTPDGVTRGSDTLLLYFTSGTTAKPKLVEHT
                     HASYPVGHLSTMYWIGLQPGDIHLNISSPGWAKHAWSNVFAPWIAESCVLVMNYSRFD
                     AASVLEVL"
     gene            1032..1493
                     /locus_tag="VFE39_09385"
     CDS             1032..1493
                     /locus_tag="VFE39_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258850.1"
                     /translation="MIDHQWRGRAAAAALLAGLLLSGCGSATPPAAPVATPPPGPTAD
                     ATTGPTTGPPIETSKPAQLPWPAAGAADAAALQAAADSGTQPWLLDPAEVAMSYAAAA
                     HGWTDAEASPEADGTSVDVRNSDGRRLALTLAQPGRTGTGGIWVVTAEQPR"
     gene            1715..2128
                     /locus_tag="VFE39_09390"
     CDS             1715..2128
                     /locus_tag="VFE39_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsgA family sporulation/cell division regulator"
                     /protein_id="HET6258851.1"
                     /translation="MTSDSVQQDMFTVLHGQPVPVVTRWTYTAADPFAISLAVGTRSD
                     RWIEWLVARDLVIDSIAGPVGEGDIRMTPQEVQGYDIVEIEIRSTDGRAVLEVDRDLL
                     QHFIEATIDIVALGDELNRMDLDDEIAKITQSCAE"
     gene            complement(2213..2425)
                     /locus_tag="VFE39_09395"
     CDS             complement(2213..2425)
                     /locus_tag="VFE39_09395"
                     /inference="COORDINATES: protein motif:HMM:NF036408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5313 family protein"
                     /protein_id="HET6258852.1"
                     /translation="MERPGPLRWLWYAMGGGLRPAHRRWVLHDLTVRTWRLRQPFRSI
                     VQILPVAVLAEATEHRALKAGFARAP"
     gene            complement(2462..3211)
                     /locus_tag="VFE39_09400"
     CDS             complement(2462..3211)
                     /locus_tag="VFE39_09400"
                     /inference="COORDINATES: protein motif:HMM:NF039203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5709 domain-containing protein"
                     /protein_id="HET6258853.1"
                     /translation="MAQRDYEEQPEAPDLGASVQLESDQQLVSPPGDRDGLDAGYVPA
                     DRPYVLDEDGVSARGMRAGDTLDQRLRRERREEPVDPDRAGRLTVSGEGAALERPDAM
                     DGVDVGIDGGAASAEEAAVHVIDGPGAPIEAEPSLAESPALDDPELDDALGGDPLAER
                     ARQEAADDVRADGEVFGPGSGRLDESGEPVGGGSADTGGGWTGRGPEPALPRDPADRD
                     PAAGIDANREVGGAAAPASGRRDVGPDTGLT"
     gene            3313..3963
                     /locus_tag="VFE39_09405"
     CDS             3313..3963
                     /locus_tag="VFE39_09405"
                     /inference="COORDINATES: protein motif:HMM:NF037150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET6258854.1"
                     /translation="MTSPTPPNSWSGAATPLPQPAAAPARNPAAARPRDSAATRQALV
                     DAGRTLFATVGYDATTVRAIADRAGVNQALLFRYFGNKEGLFAEAVLGEALELLADAP
                     RHDLLERTLAAVLDDEQGHAAEILMAVLRAAGSSQVGEAVRIRLGEAYSAAFASLVDT
                     DDPADAAVRADLLLAWLLGIALNRSVLRAGPLHDPQVITAHVLRVATALLGPRRPS"
     gene            4027..5229
                     /locus_tag="VFE39_09410"
     CDS             4027..5229
                     /locus_tag="VFE39_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6258855.1"
                     /translation="MIRAYPFGEPDGLELDPIYGELRRDEPVSRVKLPYGGEAWLALR
                     HADVRTVLGDPRFSRAAVVGKDVPRVRPEIDNQAASILNMDAPEHTRLRKLVASAFTV
                     RRVEELRPRAAELTAELLAGMRAAGSGADLVEHLSVPLPVTIICELLGVPVEDRAIFR
                     AGADAALSTSALSPSERAAAGAAMTTYMASLVAQRRAAPTDDLLGALVTARDEGDRLS
                     EGELVGLGIGILIAGHETTMNQIGNMAYSLLTRPDRGDGLRGDPEAVARGVEELLRFT
                     PLGASAGFPRIATEDVELSGVTVHAGEAVVVSVHAANRDPEVFEDPETLVLDRAVNRH
                     IGFGYGVHHCLGAQLARMELQEAVGALLREFPDLRLAVAPEDVEWRTGAFVRGPRALP
                     LAWGDDHG"
     gene            complement(5295..6020)
                     /locus_tag="VFE39_09415"
     CDS             complement(5295..6020)
                     /locus_tag="VFE39_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258856.1"
                     /translation="MLIRRRSRRNPAGLVHRIAIVLRWPIGLLAVSWRYMWRTTPIHR
                     SESDGDVHDLPPPVPPEVLDDDVQPVEDGVGPMFRRRYSVRVEGATKRPAEVMATFAG
                     DPNCSAPGEVAVFLKTRGQPGAMTAGDEFLIRMPGPWDGPVRVIDRTPTSFRLATLRG
                     HLEAGQIEFGCCPDGDWLVFEIDSCARSGDRFSHLLYSWLGLAKEIQLNLWVETCLRF
                     AARTGGRVRGGVQVHTRRLSELS"
     gene            6107..6325
                     /locus_tag="VFE39_09420"
     CDS             6107..6325
                     /locus_tag="VFE39_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258857.1"
                     /translation="MIANGALMRRAALALHGASLASIGLCVVLWLRAKTVDQDERGNA
                     ERRALFVGLWPPMFWLFGDAAERAGDRR"
BASE COUNT          892 a         2309 c         2248 g          893 t
ORIGIN      
        1 agcacctcga ggaccgacgc ggcgtcgaag cgggagtagt tcatgacgag cacgcaggac
       61 tcggcgatcc acggcgcgaa gacgttgctc cacgcatgct tggcccagcc gggggaggag
      121 atgttcaggt ggatgtcgcc gggctgcagg ccgatccagt acatggtcga caggtgcccg
      181 acggggtagg acgcgtgcgt gtgttccacg agcttcggct tcgcggtcgt gccggaggtg
      241 aagtagagca ggagggtgtc cgaaccgcgg gtgacgccgt cgggggtgaa gtccgcgggt
      301 gcagcggcgg cgccggcgta gtcgagccag ccctgcgggg caccccgcac ggcgatgcgg
      361 gtgtagtcgc cctcgacgcc gtcgaacttc gccgtgtcgg ctccaccgac caccgcgtgc
      421 ttcgccccgc cacggtcgag ccgatcgcgc aggtcggacg cggtgagcag cgtcgtggcc
      481 gggatgacga cggcgccgag cttcatcgcg gcgagcagcg tctcccacag ctcgacctga
      541 tttccgagca tgaggatgac ccggtcgccg cgggccacgc cgtgctcgcg cagccagttg
      601 gccacctggt tcgaccgcgt ggagagctcg gcgaaggtcc agtatccctc gctgccgtcc
      661 tgctcgacga tccacagcgc gcgggccgcc ccgcgctccg ggtcggccgc tacggcgtcg
      721 aaatggtcga tcgcccagtt gaactcgtcg atcttgggcc agcggaagtc gcggtaggcc
      781 gtctcgtagt cctcccggtg tgcaacgagg aactcgcgcg ccgcgtagaa agcttctgcc
      841 gaccgattca tgacctcacc ccgggatagc tgccgctgct gatcgcaccc taatgagcgc
      901 tccatctaca cagcgcggag cgctccatct acacagcgcg ggaggggtct cggaggtgag
      961 accgtccggt cccccggacg cgccggacgc gcccggccca cggaaccaga cgaaccgcct
     1021 accgttgcgg cgtgatcgat caccagtgga ggggccgcgc cgctgcggcc gctctcctcg
     1081 ccggcctgct gctctccgga tgtggcagcg ccaccccgcc cgccgcccct gttgccacgc
     1141 ccccgccggg gccgacggcg gatgcgacga ccgggccgac gacggggccc ccgatcgaga
     1201 cgtcgaagcc ggcgcagctg ccctggcccg ctgcgggcgc ggcggatgcc gcagcactcc
     1261 aggccgcggc ggacagcgga acccagccct ggctgctgga tccggcggag gtggcgatgt
     1321 cctacgccgc ggcggcgcac gggtggactg atgccgaggc ctctcccgaa gcggacggca
     1381 cgagcgtcga tgtccgcaac tccgacggac ggcgactcgc gctcacgctc gcccagcccg
     1441 gccgaacggg cacgggcgga atctgggtgg tcacggcgga gcaaccccga tgagaccacc
     1501 gaccagagca ggttcctgcg ggtgaacggg cgcgtcactg cgccgatcta gctcagctcg
     1561 gctcgacgac cgtgaccgtg tgtgatgaag gatgcctgta ctcacttgtt cgggtgaaca
     1621 ccggacgagt gtgcagcgtt ccgggtccgg ccgtcgatct cctccacaga cgccgccgca
     1681 cgagcggcgg cccgatcgcc agggggcgac gacgatgacc agcgactcgg tacagcagga
     1741 catgttcaca gtgctgcacg gtcagcccgt gccggtcgtg acgcggtgga cctacaccgc
     1801 ggccgacccc ttcgccatct ccctcgcggt cggcaccagg agtgatcgtt ggatcgagtg
     1861 gctggtcgcc cgcgatctgg tgatcgactc gatcgccggt cccgtcgggg agggagacat
     1921 ccggatgaca ccgcaggagg tgcagggcta cgacatcgtg gagatcgaga tccgctccac
     1981 cgacggtcgg gccgtgctgg aggtcgaccg cgacctgctc cagcacttca tcgaggcgac
     2041 gatcgacatc gtggccctcg gggacgagct caaccggatg gacctcgacg acgagatcgc
     2101 gaagatcact cagagttgcg ccgagtagga gcctgacccc cgcggtaccg ctgcgcgtac
     2161 cgctccgctg ccgcctcgcg ggtgcccgca tccgcctggt cgcgcacggc cttcacggtg
     2221 cccgcgcgaa cccggccttc agcgcccggt gctccgtggc ctccgccagc accgccaccg
     2281 gcaggatctg cacgatcgag cggaagggct gccgcaacct ccaggtgcgc acggtcaggt
     2341 cgtgcagcac ccaccgtcga tgcgccggcc tcagcccacc gcccatcgcg taccagagcc
     2401 accgcagcgg accgggccgt tccatttccc cgaacctatt cggcgggcgt gccaccggga
     2461 ctcacgtcag gcccgtgtcc gggccgacgt ccctgcgtcc cgatgcaggc gcggcggcgc
     2521 cgccgacctc ccgattcgca tcgatgccgg cagctggatc gcggtcggcg gggtcgcggg
     2581 gcagggcggg ttcgggtccg cgtccggtcc agccaccgcc ggtgtcggcg ctcccgccac
     2641 cgaccggctc accgctctcg tccagccggc ccgaaccggg gccgaacacc tcgccgtcgg
     2701 cgcggacgtc atcggcggcc tcctgccggg ctcgctcggc gagcgggtcg ccgccgagcg
     2761 cgtcgtcgag ctcagggtcg tccagagcgg gcgactcggc cagcgacggc tcagcctcga
     2821 tcggcgctcc gggaccgtcg atcacatgca ccgcggcctc ctcggcagac gcggcaccgc
     2881 cgtcgatccc gacgtcgacg ccgtccatcg catcaggccg ctcgagcgcg gcaccctcgc
     2941 cggagaccgt cagccgcccg gcgcggtccg ggtcgaccgg ctcctcgcgc cgctcccgcc
     3001 ggagccgttg gtcgagcgtg tcgcccgccc gcattccacg ggcgctgacg ccgtcctcgt
     3061 cgagcacgta gggccggtcc gccggcacat aaccggcgtc cagcccatcg cggtcgcccg
     3121 gaggtgaaac cagctgctgg tcgctctcca actgcacgga tgcacccagg tcgggggcct
     3181 cgggctgttc ctcgtagtct cgctgagcca tggcgcgcgg atacccacac cggtagcccg
     3241 ccacactccg ggtgtttccg cccattccgc gcggtacgtc ccgatcaccc ggcacgcgcg
     3301 ggaggagaat cgatgacgtc gccgacccca ccgaacagct ggtccggcgc cgcaaccccg
     3361 ctcccccagc ctgcggccgc cccggcacgc aacccggccg cggctcggcc gcgcgactcg
     3421 gccgcgaccc gccaggccct ggtcgacgcc ggccgcacgc tcttcgccac cgtcggctac
     3481 gacgccacga ccgtacgtgc gatcgccgac cgcgccgggg tcaaccaggc cctgctgttc
     3541 cgctacttcg gcaacaagga gggcctgttc gccgaggccg tgctgggcga ggcgctggaa
     3601 ctcctggccg acgccccgcg gcacgacctg ctggagcgca ccctggcggc ggtgctggac
     3661 gacgagcagg gccacgcggc cgagatcctg atggcggttc tgcgagcagc aggcagctcc
     3721 caggtgggtg aggccgtgcg gatccggctg ggcgaggcct actcggcggc gttcgcgtcg
     3781 ctcgttgaca ccgacgaccc ggccgacgcg gcggtccgcg ccgacctgct cctggcgtgg
     3841 ctgctcggca tcgccctcaa ccgatccgtc ctgcgcgcgg ggccgctgca cgatccgcag
     3901 gtgatcaccg cacacgtact gcgtgtcgcc accgcgttgc tcgggccgcg ccgaccctct
     3961 tgacatgcca ggacgagact tcctaccgtt cgaagtaagc ggacgcttac ttttgggggc
     4021 tttatcatga ttcgtgcgta tccgttcggg gagccggacg ggctggagct cgacccgatc
     4081 tacggggagc tgcgccgcga cgagccggtc tcccgagtga agctgcccta cggcggggag
     4141 gcctggctgg ccctccgcca cgccgacgtg cggacggtac tgggcgaccc gcggttcagc
     4201 cgcgcggccg tcgtcggtaa ggacgtcccc cgggtgcggc cggagatcga caaccaggcc
     4261 gcctcgatcc tcaacatgga cgctcccgag cacacccgcc tgcgcaagct ggtggccagt
     4321 gcgttcaccg tgcggcgggt ggaggaactg cggccacgcg ccgccgagct caccgcggag
     4381 ctgctcgccg ggatgcgcgc ggccggctcc ggtgccgacc tcgtcgagca cctgtccgtg
     4441 ccgctgccgg tcacgatcat ctgcgagctg ctgggcgtcc cggtcgagga tcgcgccatc
     4501 ttccgggccg gcgcggatgc ggcgttgtcg acgtcggcgc tgtccccttc ggagcgggct
     4561 gcggcgggcg cggcgatgac gacgtacatg gcgagtctgg tcgcgcagcg ccgcgccgcc
     4621 ccgaccgacg atctgctcgg cgcgctggtc actgcacggg acgagggtga ccggctcagc
     4681 gagggcgagc tggtcggcct ggggatcggc atcctcatcg ccggtcacga gacgaccatg
     4741 aaccagatcg ggaacatggc ttactccctg ctcacgcggc ccgaccgagg tgacgggctg
     4801 cgcggcgacc cggaggcggt cgcgcggggc gtcgaggagc tgctccggtt cacgccgctc
     4861 ggggcgtcgg ccgggttccc ccggatcgcg acggaggacg tggagctgtc cggcgtgacg
     4921 gtgcatgccg gcgaggcggt ggtggtgtcg gtacacgccg ccaaccgcga ccccgaggtg
     4981 ttcgaggacc ccgagaccct ggtgctcgac cgtgcggtca accgccacat cggcttcggc
     5041 tacggggtcc accactgcct cggcgcacag ctggcgcgca tggagctgca ggaggcggtc
     5101 ggcgcgctgc tccgcgagtt cccggacctg cgcctcgccg tggcgccgga ggacgtcgag
     5161 tggcgaacgg gcgcgttcgt ccgcggtccg cgcgccttgc ccctggcgtg gggggacgac
     5221 cacggctgag ccggttcgta ggcgggcaac cgaggacggt ggtcggacgc gcttgtcgct
     5281 ggggacaccg agcactacga gagctcgctc agccgacggg tgtgcacctg cacccctccg
     5341 cggacccgcc ctcccgtgcg agccgcgaac cgcaggcagg tctcgaccca gaggttgagc
     5401 tggatctcct tcgccagccc cagccagctg tagagcagat gcgagaaccg gtcgcccgac
     5461 cgtgcacagg agtcgatctc gaagaccaac cagtccccat cggggcagca cccgaactcg
     5521 atctgccccg cctccaggtg cccgcgcagg gtggccagcc ggaacgacgt gggggtccgg
     5581 tcgatcacgc gcaccggccc gtcccagggg ccgggcatcc ggatgaggaa ctcgtcgcct
     5641 gcggtcatcg cgcccggctg cccccgcgtc ttcaggaaca ccgccacctc cccaggggcg
     5701 ctgcagttcg ggtcgcctgc gaatgtcgcc atcacctcgg cgggacgctt cgtggccccc
     5761 tccacacgga ccgagtagcg gcggcggaac atcggtccca cgccgtcctc gacgggctgt
     5821 acgtcgtcgt cgagcacctc gggcgggacg ggcgggggca ggtcgtgaac gtctccatcg
     5881 ctctccgacc ggtggatcgg ggtggtgcgc cacatgtagc gccaggacac cgccaacagc
     5941 ccgatcggcc agcgcagcac gatcgcgatc cggtggacga gccctgcggg gttgcggcgg
     6001 gagcgccggc ggatgagcac aaaaggtggg gtacccgctt cttgtgctcg ccaacggcgc
     6061 tggtcgcgca cgcgtaggag caggagaggg cgggtactcg catcccatga tcgccaacgg
     6121 cgccctgatg cgccgcgccg cgctggcgct gcacggggcg tcgctcgcct cgatcgggct
     6181 gtgcgtcgtg ctgtggctgc gcgccaagac cgtcgaccag gacgagcggg gcaacgccga
     6241 gcgccgcgcg ctgttcgtgg gcctgtggcc cccgatgttc tggttgttcg gggacgccgc
     6301 agagcgcgca ggcgaccggc gctgagcgcc gtgcgggacc tc
//
LOCUS       DASRSC010000191        16251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1868480,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000191 DASRSC010000000
VERSION     DASRSC010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 16251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16251
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868480"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VFE39_09425"
     CDS             <1..1306
                     /locus_tag="VFE39_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6258858.1"
                     /translation="LGAVPRVDTAERRPLLPINGNPPLLVDLPAGCPFRPRCPISVPE
                     CATEEPKLAPVSAPVLEGMHAVACIRAGHIDGGRVDGVAIYPVPPVPVAPSAAIPREQ
                     RPAVLELDGISKTFPLLKGKLLRRRVGSVNAVSGVHLDIRQGESLGLVGESGCGKTTT
                     LLEIMNLREPERGSIRVAGVDVADLKGRAQERALRRKLQMVFQDPMGALDPRLTVFDI
                     LAEPLRAVGEADRAAIGHRIDELMELVGLDPEHSDRFPTAFSGGQRQRIGIARALATN
                     PSLVVLDEPVSALDVSIQAGVINLLNDVKVELGLSYLFVAHDLSVVRHISDRVAVMYL
                     GRIVEIGAVDAVFDNPRHPYTQALLSAIPVPDPQVERSRERVVLRGDLPSPTDSSPGC
                     PFVSRCPLHPTLEPAQRARCISETPELTARADTDQLNACHFR"
     gene            1372..3045
                     /locus_tag="VFE39_09430"
     CDS             1372..3045
                     /locus_tag="VFE39_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter family substrate-binding
                     protein"
                     /protein_id="HET6258859.1"
                     /translation="MHVLKRTAALAATAVLSVVLAACGSAGSNGGDVSAQSLAAQVAY
                     NPQPYDNIKDGGTLTTSMPEISPQFNSWQGDATAYTGEVWGWYNPVLITFTADGQAVF
                     NPDYLTDVKQETVGGNTRVTYTINPKATYNDGTPIDWTSFEAVWKANRGTDPAYIVNS
                     SDGYDQIRSVTQGVNARQAVVTFNGLDLWWQNLFGTVLNPKVLAPALFNQGYINNPHP
                     EWGAGPYTVSKFDQLNGVITFERNPKWWGKRGKLDSMTFLAMESSADINAFKNGQLDA
                     VSVGSKDRLAQVTGQPGTEIRKSASLQLDYFTLNGTSPVLSDIQVRKAIFEGIDRNEI
                     AKVQLQGLGYTADPAGSMTLLPFQEGYQDDFDKVVTFDPEQAKKDLDAAGWPVGPDGI
                     RVKNGRPLEFSYVNTGDDPIAKAVAGATAAMLKNIGIKLDIRQVPSSDFSKIVAGQQF
                     DMFYSGVAESSPYGIASICQLFCSDSQFIKSGVNSPANDTLIRSVNTLPTPQEQYTRA
                     SEAETAAFTTFGVMPTVNLPAIDAVKKGLANYGPGRYFSTTPENIGWQK"
     gene            complement(3068..4084)
                     /locus_tag="VFE39_09435"
     CDS             complement(3068..4084)
                     /locus_tag="VFE39_09435"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6258860.1"
                     /translation="MFFALTEDQREFDAAVRGYLADRFDLEAVRAVVEDPSGDGHPAS
                     LWKAAAEQGWLAVLVPEEHDGLGLGLVEAQVIARALGAGVAPGPWRGTVLAAEAIRLA
                     GSDEQRAAWLPRLAGGDAVGALTLRGSVPGTLPAVEYGEIADVVVARSADGLALVEGA
                     SAVRQGSYDGSVRLAAVEAAESQALPGATAEVIDELAARATVLVAADLVGIAREAISR
                     TVAYDRERQQFGVPVGSFQAIKHALADLHIGVTMAEHAALYAAHAVDGGSADAPLAVS
                     VAKAKASDVAIGATAAMIQYHGGIGYTWEHEAHFYYKRAKRLAGQFGDADAHRARIAD
                     LTMR"
     gene            complement(4094..5263)
                     /locus_tag="VFE39_09440"
     CDS             complement(4094..5263)
                     /locus_tag="VFE39_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6258861.1"
                     /translation="MDLTYTAAEEEFRGELRSWLRSHIPEEWTRPGFWESLDDDESFR
                     LRRDWERAKALAGFAGIEWPTEFGGRGGTPGMRAIYDEEMVLARAPRTVNPLGLSFLA
                     PTVMAIGTDAQKKEIIGPLLRNEVIWCQGFSEPGAGSDLAALSTRGVRDGDDFVVNGQ
                     KVWTTNAVHGDKIFTMVRTDAGSAKHRGISMLLIDMHQPGVEARPLKQMSGASEFGEV
                     FFTDARVPAVDCLGEIGDGWRTAMLLLSFERGASGIAQYTEFRRQYDEIVAVAKKLGR
                     DGDPAVRDKLARVLTELECLRLHAMHVLTQVEQGRDLGFEASMTKLQWSETFQDLWET
                     FDDVLGEDATLDAIDGLDLRPLHAQSMWSRSVTIWGGSSQVQRNITAERVLGLPR"
     gene            5394..5636
                     /locus_tag="VFE39_09445"
     CDS             5394..5636
                     /locus_tag="VFE39_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258862.1"
                     /translation="MRTERVTVSLPAELVDEARNAVLRGTARSISAYIAEAVSARQLR
                     DRSLATLADLYGGPPPPDELAEARRTLRLVPPAAAV"
     gene            5752..6144
                     /locus_tag="VFE39_09450"
     CDS             5752..6144
                     /locus_tag="VFE39_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258863.1"
                     /translation="MIGGRVLDTTALAAFAIGRPVYVRALVWAAVEENIVLAVPSAAL
                     GRAWSLLEPEHHAALQVLLDLPNTVIDELGPASALESGLLLAASGQDDIVAGQVAASA
                     RRRGWPAVTGDPATLRKLDTTVTIEELP"
     gene            complement(6156..6914)
                     /locus_tag="VFE39_09455"
     CDS             complement(6156..6914)
                     /locus_tag="VFE39_09455"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HET6258864.1"
                     /translation="MSASVERWLTFEGLDNVRDIGGLPLRGGGTTRPGLLLRSASLHH
                     CTPADVEHLVDVFGLRLVLDLRTPREIEQDGPTALARAGVETVALSFLPEQGRTLPET
                     DDDTDPLVRNYLGYLSDRGENVVTAVRLLAAPDAGPSLVHCAAGKDRTGVLVALMLDA
                     AGVERDAVVADYALSAEQVEALFRRWTTASGEPMPEDIARHLPRAEAMAAVLAHLDAE
                     YGANSSGGAAGWLRANGLDEESLTRLRARLTGAP"
     gene            complement(6974..7300)
                     /locus_tag="VFE39_09460"
     CDS             complement(6974..7300)
                     /locus_tag="VFE39_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HET6258865.1"
                     /translation="MIYEIREYTTVPGRMPALVKRFNEHTFGVFRKHGMEVAFISLTE
                     LGGNSSNELVYVMKFDSYEDMASKWAAFQADPDWRAARSASEADGPIVASISRRVLSP
                     AAFAAR"
     gene            7511..8635
                     /locus_tag="VFE39_09465"
     CDS             7511..8635
                     /locus_tag="VFE39_09465"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HET6258866.1"
                     /translation="MTDPRTSLIRQQLADLGRRHAAEESRGATTPRATRVLIIGNFGN
                     GNTGDEAMLARTLQELPANTEVRVVSRNPRMVEAMHRVPAVAMEGIAFTKALKWCDGI
                     AVVGGGLFGTGASSLVKALPAIVWAATARGRDITYVAIGVYPGTPERVLDLLRRSVRK
                     PGRISVRDEVSAATLGNAIVAPVVGDLAMGLTPAPAADARRALAAAGVDPAVPLLLVA
                     PKALPNPILVDCLQDAAETLARRWIERGGAVAGLAISTHADYGLGLARRDEVLIEELG
                     ERLGRRVPVLGPQLPPALAKAVVGEADALFGLRIHALIFAVSTGVPCLGVGWEERTTA
                     FLAEHEQSAIGTRPPAEELHAWVDRSLPARWSARPRLLSS"
     gene            complement(8653..9123)
                     /gene="soxR"
                     /locus_tag="VFE39_09470"
     CDS             complement(8653..9123)
                     /gene="soxR"
                     /locus_tag="VFE39_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HET6258867.1"
                     /translation="MDTRDLLTIGEVAHRSGFPPSALRYYEREGLLHTTRTSGGQRRY
                     ERSVLRRLAFIRAARTLGVGLDEVREGLDQLPDGRTPNKADWSRLSRSWRGRLDEQIQ
                     ALVALRDGLDSCIGCGCLSLQRCRLYNPGDRVAELGPGARLLPPMLRRTPAEGV"
     gene            9171..9908
                     /locus_tag="VFE39_09475"
     CDS             9171..9908
                     /locus_tag="VFE39_09475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6258868.1"
                     /translation="MNGTIAIVTGAGQGFGLALTAALAARGSRVVACSRDADRLLAAT
                     AGLPGVVALPGDITDPEFRAELVSTAWRLDLLVHNAGELGPSPLPSLEHFPLDALRGV
                     LETTVLAPLALTQLALPLLRSSTAGTVITLSSDAAVEAYPGWGGYGAAKAALDHVSAV
                     LAVEEPALRVYAFDPGDMRTAMHQRAFPGEDISDRPDPMTVVPALMRLLDRRPESGRY
                     RAADLMDDGQQAQRAPGTPDRPAGSTA"
     gene            9905..11008
                     /locus_tag="VFE39_09480"
     CDS             9905..11008
                     /locus_tag="VFE39_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HET6258869.1"
                     /translation="MRAAAATTFAVPPELSATEPPEVRGLARDGVRLLVARDRRPLQH
                     TTFRDLPLALHRGDLVVVNTSDTEPAAVDGHRTGRSDGRSADGPPVTLHISGPAPDAL
                     FVVELRTPDGRRVTDGRAGESVRLPGGVVATLLTGHPDPWVVTGSRLWRARIPVAGGL
                     RAWLAAVGRPIRYSYLRGDWPLDAYRTVFAAPGPEFASAEMPSAGRPFTAAVLDGLRR
                     RGVGVAELVLHTGVSSLEPDEVPLPERYRVPLATADAVNATRAAGGRVVAVGTTVARA
                     LETVADPAGRVHAGEGWTHLVLGPDRPSRVVTGLLTGWHEPQASHLLLLEAVAGARLV
                     GRAYAAAVDRGYLWHEFGDSCLLFGRQTARRRV"
     gene            11529..12035
                     /locus_tag="VFE39_09485"
     CDS             11529..12035
                     /locus_tag="VFE39_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HET6258870.1"
                     /translation="MSFAVGTTDPSSGPDEQLASLDKATSLAADLAQLHAKQAREQAD
                     RDRVQAVIDSGGLDGWIAEALQIMDMPQSYASGLKRIIMAESGGNPRAINTWDSNARR
                     GTPSQGLMQTIPSTFRAYVHPSLAGRPITDPVANITAGVRYMIANYGIGTLKAGGRSN
                     SSGDYVGY"
     gene            complement(12148..12378)
                     /locus_tag="VFE39_09490"
     CDS             complement(12148..12378)
                     /locus_tag="VFE39_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET6258871.1"
                     /translation="MPRSPILDVPISEDPIRLGQFLKLAGLAEHGGHAKALLEAEEVL
                     VNGRVETRRGTQLRAGDVVAIGDRRARPVPLV"
     gene            complement(12501..13433)
                     /locus_tag="VFE39_09495"
     CDS             complement(12501..13433)
                     /locus_tag="VFE39_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6258872.1"
                     /translation="MPSRERQFEHPALFYTGLDDYLAGTLSFIHEGLGADEPVAVAVP
                     GSRLEPLRAELGSVADQVRFLDMNEAGRNPGRILPGVLLAFADEHPDAARVRIIGEPI
                     WPGRSATEYPACVQHEALINLAFAGRPATILCPYDAEGLPPEVLADAEATHPMVLDAG
                     APRASTSYAVDRVLAECNRPLPELTEHPPLRVDESMLSRARGFAHAEAGRAGLGEDRV
                     ADVEMVVNELVTNSVEHGGGMGILRIWIEDAHLVVEVRDVGQLHDPLLGRRPLRPLQA
                     RGRGLFLVNHLADLVRLHTGPDGTTMRAYFGLPT"
     gene            complement(13624..13962)
                     /locus_tag="VFE39_09500"
     CDS             complement(13624..13962)
                     /locus_tag="VFE39_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258873.1"
                     /translation="MDGAGGAVASPRRTDGSACIGDDSGHLRRDLDSGVAAAQAAAGD
                     TYVNVLGARIARQSLDAGVLDEIFVRIAPVLLCDGVRLFDHQGGTNVKLERLSPTHAP
                     LATNIRLRIV"
     gene            complement(13902..14357)
                     /locus_tag="VFE39_09505"
     CDS             complement(13902..14357)
                     /locus_tag="VFE39_09505"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HET6258874.1"
                     /translation="MTAAAHDERHALLEELGLLRARRRELEESLVADDHRPHDFGEEG
                     LTTELRDEIDWIDRRIRDVVHYLWEAGRPEGDTSERVGPYSVVTLRFPDGSSESAQVT
                     RFPDEDVPVVTPESPLGRALLGAQPGDEITWTAPEGRLRARVERMDRPA"
     gene            14641..15072
                     /locus_tag="VFE39_09510"
     CDS             14641..15072
                     /locus_tag="VFE39_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET6258875.1"
                     /translation="MPTTSDFERMLRGVALRVTRPRVAVLSAVHDHPHADTASIIGVV
                     REDLGEVSHQAVYDVLRALTAAGLIRCIQPPGSLARYESRVRDNHHHVVCRSCGAIAD
                     VDCAVGTTPCLTASHDHGFAIDEAEVIYWGLCPDCSPAVSS"
     gene            15101..>16251
                     /locus_tag="VFE39_09515"
     CDS             15101..>16251
                     /locus_tag="VFE39_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HET6258876.1"
                     /translation="MSDSPNAVVGEMNEEQEARCPVATGRRPHPTEGGSNRDWWPNQL
                     NLKILRKHPAVADPMGEDFDYAAAFNTVDLDALARDVDEVLTTSQEWWPADFGHYGPF
                     MIRMAWHSAGTYRIHDGRGGAGAGMQRFAPLNSWPDNGNLDKARRLLWPVKKKYGRKV
                     SWADLMIFAGNRALETMGFTTFGFAGGRADVWEPDEDVYWGPERTWLGDERYTGDREL
                     ENPLAAVQMGLIYVNPEGPNGTPDPLAAARDIRETFGRMAMNDEETVALIAGGHTFGK
                     AHGAADPDRYVGAEPEGAPLELQGLGWASSFNSGKGEDAITSGLEVTWTDRPTEWDNG
                     FFDILFGYEWELTKSPAGAHQWKPKNDGGANTVPDPESGELVRTPTMLTT"
BASE COUNT         2453 a         5912 c         5552 g         2334 t
ORIGIN      
        1 gctcggtgcc gtcccgcggg tggacaccgc cgagcgccgt ccactcctgc cgatcaacgg
       61 caacccgccg ctcctggtcg acctacctgc gggctgcccg tttcggcccc gctgcccgat
      121 ctcggtgccg gagtgcgcaa ccgaggaacc gaagctggcg ccggtgtccg ccccggttct
      181 cgaggggatg catgcggtgg cctgcatccg cgccggtcac atcgacggcg gccgggtcga
      241 cggcgtggcg atctacccgg tgccacccgt gccggtggcc ccatcggcag cgattccccg
      301 cgagcagcgt cccgccgtgc tcgagctgga cgggatcagc aagacgttcc cgcttctcaa
      361 gggaaagctg ctgcgacggc gtgtggggtc ggtgaacgca gtctcgggtg tccacctcga
      421 catccgccaa ggcgagtcgc tcgggctggt gggcgaatca ggttgcggga agaccaccac
      481 cctgctggag atcatgaacc tccgtgagcc cgagcggggc agcatcaggg tcgcgggcgt
      541 tgatgtcgcg gacctgaagg gaagagctca ggaacgtgct ctgcgacgca agctgcagat
      601 ggtgttccag gacccgatgg gggcgctcga cccgcggctg acggtcttcg acatcctggc
      661 ggagccgttg cgggccgtgg gcgaggcgga ccgggccgcg atcgggcacc ggatcgacga
      721 gttgatggag ttggtcgggc tcgaccccga acacagcgac cggttcccca ccgccttctc
      781 cggaggacag cgccagcgca tcggcatcgc ccgcgccctg gcaaccaacc cgtcgctggt
      841 ggtgctggac gagccggtct cggccctgga cgtctccatc caggccggcg tgatcaacct
      901 cctgaatgat gtgaaggtcg agctcgggct gtcctacctg ttcgtcgcgc acgacctttc
      961 cgtggtgcgg cacatctcgg atcgggtggc cgtgatgtac ctgggccgca tcgtcgagat
     1021 cggggccgtc gacgcggtct tcgacaatcc gcgacacccg tacacccagg cgctcctctc
     1081 ggcgatcccg gttcccgacc cgcaagtcga gcgctcgaga gaacgggtgg tgctccgcgg
     1141 cgacctgccc agcccgaccg actcctcccc cggctgcccg ttcgtcagcc ggtgtcccct
     1201 gcacccgacc ctcgaacccg cgcagcgcgc ccgctgcata agcgagaccc cggagctgac
     1261 cgccagggcg gacaccgacc agctgaacgc ctgtcacttc cgctgatgct cggcgaggca
     1321 gatccgcggt ggattcggcg cacccaatac ctcatcagaa ggaaaccgac catgcacgtc
     1381 ctgaagcgaa ccgctgcgct cgcggccacc gccgtgctgt cggtggtgct ggcggcctgc
     1441 ggcagcgccg gatcgaacgg gggcgatgtc agcgcccaat cgctcgcggc ccaggttgcg
     1501 tacaacccgc agccctacga caacatcaag gatggtggca cgctcaccac gtcaatgccg
     1561 gagatctccc cccagttcaa cagttggcag ggcgacgcca ccgcttacac gggggaggtg
     1621 tggggctggt acaacccggt gctcatcacg ttcacggctg acgggcaggc cgtcttcaac
     1681 cccgactacc tcaccgacgt gaagcaggag accgtcggcg gcaacacccg ggttacctac
     1741 acgatcaatc cgaaggccac ctacaacgac ggcactccga tcgactggac gtccttcgag
     1801 gcggtgtgga aggccaaccg gggtactgat ccggcctaca tcgtcaactc gagcgacggt
     1861 tacgaccaga tccggtcggt gacgcaggga gtgaacgcac gccaagcggt ggtcacgttc
     1921 aacggcctcg acctgtggtg gcagaacctg ttcggcactg tgctcaaccc gaaggtcctc
     1981 gcccccgcgc tgttcaacca ggggtacatc aacaacccgc acccggaatg gggtgccggc
     2041 ccctacaccg tcagcaagtt cgatcagctg aacggcgtca tcaccttcga gcgcaatccg
     2101 aagtggtggg gaaagcgggg caagctcgac tcgatgacgt tcctcgcgat ggagtcctcg
     2161 gccgacatca atgcgttcaa gaacggccag ctcgacgccg tgtcggtcgg cagcaaggac
     2221 cggctggcgc aggtcaccgg gcagccaggc accgagatcc gcaagagtgc ctcattgcag
     2281 ctcgattact tcacactcaa cggcaccagc cccgtcctct ccgacatcca ggtgcgcaag
     2341 gcgatcttcg aaggcatcga ccgcaacgag atcgccaagg tccagctcca gggcctcggc
     2401 tacacggccg acccagccgg ttcgatgacc ctgctgccct tccaggaggg ctaccaggac
     2461 gacttcgaca aggtcgtcac cttcgacccc gagcaggcga agaaggatct cgacgccgcc
     2521 ggatggccgg tcggcccgga cggcatccgg gtcaagaacg gccggcctct cgagttcagt
     2581 tacgtcaaca ccggggacga ccccatcgcg aaagcggtcg ccggagcgac cgcggccatg
     2641 ttgaagaaca tcggcatcaa gctggacatc cgacaggtcc cgtcgagcga cttctccaag
     2701 atcgtcgccg ggcagcagtt cgacatgttc tactccggcg tggcggagtc cagcccgtac
     2761 gggatcgcca gcatctgcca gctgttctgc tcggactccc agttcatcaa gtccggcgtc
     2821 aacagccccg cgaacgacac gctgatcagg tccgtgaaca cgttgcccac gccgcaggag
     2881 cagtacacga gggcttccga agcggagacc gcggctttca cgaccttcgg agtcatgccc
     2941 acggtgaacc tgccggcgat cgacgcggtg aagaagggcc tggccaacta cgggccgggg
     3001 cgctacttca gcacgacgcc ggagaacatc ggttggcaga agtagcactc gtgcgcggac
     3061 gggcgcgtca gcgcatggtg aggtcggcga tgcgggcccg gtgggcatcg gcgtcgccga
     3121 actggccggc cagccgcttg gcgcgcttgt agtagaagtg cgcctcgtgc tcccaggtgt
     3181 acccgatccc gccgtggtac tggatcatcg cggcggtcgc cccgatggcg acgtcgctcg
     3241 ccttggcctt cgccaccgag accgcgagcg gggcgtcggc cgacccgccg tcgacggcgt
     3301 gggctgcgta gagcgcggcg tgctccgcca tcgtcacgcc gatgtgcagg tcggccagcg
     3361 cgtgcttgat cgcctggaac gacccgaccg ggacgccgaa ctgctgccgc tcccggtcgt
     3421 aggcaacggt gcgcgagata gcctcgcgcg cgatgccgac caggtcggca gcgacgagaa
     3481 cggtggcgcg cgctgcgagc tcgtcgatca cttcggcggt ggcccccggc agtgcctggc
     3541 tttcggcggc ctccaccgcg gcgagccgca cgctgccgtc gtaggagccc tggcgaacgg
     3601 cggacgcgcc ctcgaccagt gcgagcccgt cggcggagcg ggcgacgacg acgtccgcga
     3661 tctcgccgta ctccactgca ggaagcgtgc ccggcacgct gccgcgcagg gtcagggcgc
     3721 ccacggcgtc accaccggcc agccgcggga gccaggccgc ccgctgctcg tccgaccccg
     3781 ccaggcggat cgcctccgcg gcgagcaccg tgccccgcca tggccccggc gccacgccgg
     3841 cgccgagcgc gcgggcgatc acctgcgcct ccaccagccc cagcccgagc ccgtcgtgct
     3901 cctcgggcac gagcaccgcc agccagccct gctcggcggc ggccttccac agcgaggcgg
     3961 ggtgcccgtc gccggacgga tcctcgacga cggcgcggac ggcctcgagg tcgaaccgat
     4021 cggcgaggta gccgcgcacc gcggcgtcga actcacgctg gtcctcggtc agtgcgaaga
     4081 acatgaccag ctcctatcgc ggcaggccga gcacgcgctc ggcggtgatg ttgcgctgca
     4141 cctgcgagga cccaccccag atcgtgacgg atcgggacca catcgactgc gcgtgcagcg
     4201 gccggaggtc gagaccgtcg atcgcatcga gggtggcgtc ctcgccgagg acgtcgtcga
     4261 aggtctccca caggtcctgg aaggtctcgg accactgcag cttggtcatc gacgcctcga
     4321 aaccgaggtc acgcccctgc tccacctgcg tcagcacgtg catggcgtgc agtcgcaggc
     4381 attccagctc ggtgaggacg cgcgcgagct tgtcacgcac cgccgggtcg ccgtcgcggc
     4441 cgagcttctt cgccaccgcg acgatctcgt cgtactgccg gcggaactcc gtgtactggg
     4501 cgatgccgga cgcgccgcgt tcgaaggaga gcagcagcat cgccgtgcgc cagccgtccc
     4561 cgatctcccc gaggcagtcg acggcgggca cgcgggcgtc ggtgaagaac acctcgccga
     4621 actcgctggc cccgctcatc tgcttgagcg gccgcgcctc gacgccgggt tggtgcatgt
     4681 cgatgagcag catcgagatg ccgcggtgtt tggccgaccc ggcatccgtg cggaccatgg
     4741 tgaagatctt gtcgccgtgc acggcgttgg tggtccagac cttctggccg ttgaccacga
     4801 agtcgtcgcc gtcgcgtacg cccctcgtgg acagggcggc gaggtcggag cctgcgccgg
     4861 gctcggaaaa gccctggcac cagatcacct cgttgcgcag cagcggcccg atgatctcct
     4921 tcttctgggc gtccgtgccg atggccatca cggtgggggc gaggaacgac aggccgagcg
     4981 ggttgacggt gcgcggtgcg cgcgccagca ccatctcctc gtcgtagatc gctctcatgc
     5041 cgggtgtccc gccgcgaccg ccgaactccg tgggccactc gatgcccgcg aagccggcca
     5101 gcgccttggc acgctcccag tcgcgacgca gccgaaagct ctcgtcgtcg tcgagggact
     5161 cccagaaccc cggccgggtc cactcctcgg ggatgtggga ccgcagccac gaccgcagct
     5221 cgccgcggaa ctcctcttcc gctgcggtgt acgtcaggtc catcgtgacc tcctcgtcgg
     5281 cacgcttccg ggctccgtcg accaccttgg catacccgtc agtaaccggc gtgctccctg
     5341 ccccttggtt gacggtgcgt gagcactacc agtgcggcgg gtacgctccg cacatgcgca
     5401 ctgagcgtgt cacggtgagt ctcccggccg aactggtcga tgaggcccgc aacgccgtgc
     5461 tgcgcgggac ggccaggagc atctcggcct acatcgccga agccgtctcc gcccggcagc
     5521 tccgggaccg gtcccttgcc accctcgccg acctgtacgg cggtccaccg ccacccgatg
     5581 agctcgctga ggcgcggcgg acgctgcgcc tggtgccacc cgcggccgca gtgtgatggg
     5641 cgccgtccac accggcgctc tccacaggga cgcactccag acggtccacc gctgtcccgg
     5701 acaccgcgtg atggccgccc gccacccggg cgtcgtcgcg gcgggcgccg catgatcggc
     5761 gggcgggtcc tcgacaccac cgcactggcc gccttcgcca tcggcaggcc cgtctatgtg
     5821 cgggcgctgg tctgggctgc ggtcgaggag aacatcgtgc tcgcggtccc gagcgccgcg
     5881 ctcggtcggg cgtggtcgct gctcgaaccc gagcatcacg cggccctgca ggtgctgctg
     5941 gacctgccca acaccgtgat cgacgagctc ggccccgcct cggcgctgga gtccgggctg
     6001 ctacttgccg cctcgggaca ggacgacatc gtggccggcc aggtggcggc gagcgcccgc
     6061 cgcaggggct ggcccgcggt gaccggcgat cccgccacgc tgcgcaagct cgacacgacc
     6121 gtgacgatcg aggagctccc ctgacgcctg gggagctacg gggcgcccgt caggcgggcg
     6181 cgcagccgcg tcagtgactc ctcgtccagg ccgttcgcgc gaagccaacc cgcggctccg
     6241 ccggaactat tcgcgccgta ctcggcatcg aggtgggcga gcaccgcggc catggcctcg
     6301 gcgcgcggca ggtgccgggc gatgtcctcc ggcatcggct cacccgatgc cgtggtccac
     6361 cggcggaaca acgcctccac ctgctctgcg gagagcgcgt agtcggccac caccgcatcg
     6421 cgctcgacgc cggccgcgtc cagcatcagc gcgacgagca cacctgtgcg gtcctttccg
     6481 gccgcgcagt gcacgagcga gggaccggcg tccggcgccg ccaggagccg taccgcggtg
     6541 acgacgttct caccgcggtc gctcaggtag ccgaggtagt tgcgcaccag cgggtcggtg
     6601 tcgtcgtcgg tctcggggag cgtccgtccc tgctccggga ggaagctgag cgccaccgtc
     6661 tcgaccccgg cacgggcgag ggccgtcggc ccgtcctgct cgatctcccg gggcgtgcgc
     6721 aggtccagca ccagccgtag cccgaacacg tcgacgaggt gctcgacatc ggcgggcgtg
     6781 cagtggtgca gcgaagcact acgcagcagc aggcccggcc gggtggtgcc gccgccgcgc
     6841 agcggtaggc caccgatgtc gcggacgttg tcgagaccct cgaaggtgag ccagcgctcc
     6901 acggatgcag acactccgtc accctcgctc attcgatcga cttccgcacc cctgtgtacg
     6961 cgatcggccc gatctatcgg gcggcgaacg cggcggggct gagcacccgc ctgctgatcg
     7021 acgcgacgat cggcccgtcg gcctcgcttg cgctgcgcgc cgcccgccag tccgggtcgg
     7081 cctggaacgc cgcccacttc gacgccatgt cctcgtagga gtcgaacttc atcacgtaga
     7141 cgagctcgtt gctggagttg cctcccagct ccgtgaggga gatgaaggcg acctccatgc
     7201 cgtgcttccg gaagacgccg aacgtgtgct cgttgaaccg cttcaccagg gcaggcatcc
     7261 gcccgggcac cgtcgtgtac tcacggatct cataaatcat cagacaatga tatgggtatg
     7321 tgggacgact gccagacgat cgtacccggc gtacggccac cgcccgatag gggccgttcg
     7381 gcgaaggtgc cgcccgcgcg gccgccggga caccgtaggc tgcggcgaac gagtgaccgt
     7441 ggctacgcag ctcctccgca cctccggccg atggtcacag agagtcgcac cgcccgaacc
     7501 gaggaactcg atgacggatc cccgcaccag cctcatccgc cagcagctcg ccgatctcgg
     7561 caggcggcac gccgccgagg agagccgtgg tgccaccact ccgcgtgcca cccgtgtgct
     7621 gatcatcggc aacttcggca acggcaacac cggcgacgag gccatgctcg cccgcacgct
     7681 gcaggagctc cccgccaaca cggaagtgcg tgtcgtgtcc cgcaacccgc ggatggtcga
     7741 ggccatgcac cgggtaccgg cggtcgcgat ggagggcatc gcgttcacga aagcgctcaa
     7801 gtggtgcgat ggcatcgccg tggtcggcgg aggcctgttc ggcacggggg cgtcgtcgct
     7861 ggtcaaggcc ctcccggcga tcgtctgggc cgcaacggca cggggtcgcg acatcacgta
     7921 tgtggcgatc ggcgtctacc ccggcacgcc cgagcgggta ctcgacctgt tgcgtcgctc
     7981 ggtccgcaag ccgggccgga tcagtgtgcg cgacgaggtg tcggcagcca cgctgggcaa
     8041 cgcgatcgtg gcgccggtgg tgggcgacct tgcgatggga ctcacgcccg ccccggccgc
     8101 cgacgctcgg cgcgcgctcg cagcggccgg tgtcgaccca gccgtgccgc tgctgctcgt
     8161 cgcgcccaag gcgctgccca acccgatcct cgtcgactgt ctgcaggacg ccgccgagac
     8221 gctggcccgg cgctggatcg agcgtggtgg agccgtcgcc ggactggcga tcagcacgca
     8281 cgccgactac gggctggggc tcgcccgccg cgacgaggtg ctgatcgagg agctcggcga
     8341 gcgcctgggc cggcgggtcc cggtgctcgg cccgcagctg ccgccagcgc tcgccaaggc
     8401 cgtggtcggc gaggccgatg cactgttcgg cctgcggatc cacgcgctga tcttcgccgt
     8461 gtccacgggg gtgccctgcc tcggcgtggg ctgggaggag cgcaccaccg ccttcctcgc
     8521 cgagcacgag cagtcggcga tcggcacccg gccgcctgct gaggagctgc acgcctgggt
     8581 ggatcggtcg ctgccggccc gctggtccgc gcgcccgcgt ctgctctcct cgtagctcgc
     8641 gcgtccgggg gctcagactc cctcagccgg ggtgcgccgc agcatcggcg gcagcagcct
     8701 cgcgccgggg ccgagttcgg cgacccggtc gccggggttg taaagccggc agcgctgcag
     8761 cgagaggcag ccgcagccga tgcacgagtc gagcccgtcg cgcagggcca ccagcgcctg
     8821 gatctgctcg tcgaggcgcc cgcgccagga ccgcgacagc cgtgaccagt ccgccttgtt
     8881 cggggtccgg ccgtcgggga gctggtcgag gccttcgcgt acctcgtcca ggccgacgcc
     8941 gagcgtgcgt gccgcccgga tgaacgcgag cctgcgcagc accgaacgtt cgtaccggcg
     9001 ctgtcccccg gacgtacggg tggtgtgcag cagcccttcc cgctcgtagt accgcagcgc
     9061 cgacggtggg aagccgctgc ggtgcgccac ctcgccgatg gtgagcaggt ccctggtgtc
     9121 catcccgtct cccttgacct gaagctggct tcaagtagca cggtagtctc atgaacggga
     9181 caatcgccat cgtcaccggt gccgggcagg gcttcgggct cgccctcacc gccgccctcg
     9241 ccgctcgagg cagccgggtc gttgcctgct cccgggacgc cgaccggctg ctcgccgcca
     9301 cggccggcct gccgggcgtg gtcgccctgc ccggcgacat caccgacccc gagttccggg
     9361 ccgagctcgt cagtaccgcg tggcggctcg acctgctcgt gcacaacgcc ggcgagctcg
     9421 gtccgtcgcc gctgcccagc ctcgagcact tcccgctcga cgcgctgcgc ggcgtgctcg
     9481 agacaaccgt gctggcccca ctggcactca cccagctcgc gctgccgttg ctgcgcagct
     9541 cgaccgcggg caccgtgatc actctgagct ctgacgcagc cgtcgaggcc tatccgggct
     9601 ggggcggcta cggtgccgcg aaggcggcgc tcgaccatgt ctctgcggtg ctcgccgtcg
     9661 aggagccagc cctgcgggtc tacgccttcg accccggcga catgcgcacg gcgatgcacc
     9721 agcgggcctt ccccggtgag gacatctccg accgccccga cccgatgacc gtcgttccgg
     9781 cgctcatgcg cctgctcgac cggcgtccgg agagcggccg ctaccgcgca gcggacctta
     9841 tggacgacgg gcagcaggcg cagcgggcac ccgggacacc ggaccggcct gcggggagca
     9901 cagcgtgaga gccgccgccg ccacaacgtt cgccgtgccg cccgagctgt cggccaccga
     9961 gcctcccgag gtccgcggcc tggcccgcga cggcgtccgg ctcctggtcg cccgcgaccg
    10021 gcggccgctg cagcacacga ccttccgcga cctcccgctg gcgctgcacc gcggtgacct
    10081 cgtggtcgtc aacacctccg acaccgagcc tgccgccgtc gacgggcacc ggacgggccg
    10141 cagcgatggg cgctcggccg acggaccgcc cgtcaccctg cacatctccg gccccgcgcc
    10201 cgacgcgctg ttcgtcgtcg agctacggac gccggacggg aggcgggtca ccgacggccg
    10261 cgcgggcgag tccgtccggt tgccgggtgg cgtggtggcc acgctgctga ccggccaccc
    10321 ggatccctgg gtcgtcacgg gtagcaggct atggcgggca cggataccgg tggccggcgg
    10381 gctgcgggcg tggctcgcag cggtgggacg accgatccgg tactcctacc tgcgcgggga
    10441 ctggccgctc gacgcgtacc ggaccgtctt cgcggcgccg gggccggagt tcgccagcgc
    10501 cgagatgccc agcgcgggcc gcccgttcac cgcggccgtg ctggacgggc tgcgcaggcg
    10561 cggcgtgggg gtggcggagc tggtgctgca cacgggtgtg tcgtcgctcg agcctgacga
    10621 ggtgccgtta cccgagcggt atcgggtgcc gctcgcgacg gccgacgccg tcaacgcgac
    10681 cagggcagcg ggtggccggg tggtcgcggt cggcaccacc gtcgcgcgcg cgctcgagac
    10741 ggtggccgac ccggcgggcc gcgtacacgc cggggagggc tggacgcatc tggtgctcgg
    10801 gcccgaccgt ccgtcgcgcg tggtgaccgg gcttctgacg ggctggcacg agccgcaagc
    10861 ttcccacctg ctgcttctcg aggcggtcgc gggcgcgcgg ctcgtcggcc gggcctatgc
    10921 ggcagccgtc gaccggggtt atctgtggca cgagttcggg gactcgtgcc tgctgttcgg
    10981 gcggcagacc gctcgccgac gcgtgtaaac gttccgcctc gttcgtagtt attcgcctgt
    11041 cccgatcctg cgagccgaac ggcaggcggg gtgcggtggc cgggcacccc cggtcttgct
    11101 cgggagcgac gcgcgccaat aaggtcacct cagcgtcacg atccgatcat gaacgcaacc
    11161 gagccgggac tccccacccg gctcaaccgg cctccccacc ggcagcgtga gcggatcgct
    11221 cccccgcgct gaaagggcgt tcgttggcag gacaccgctc cccccgtggt cgtctagcgc
    11281 gccgcactgt cgaccccgta tccggccggc accagaccac tcccccaccc accggcccag
    11341 tcctgcgcac caccgttctc gccaccgctg ccgccgtcgg tgcggttgcc accggcacgt
    11401 tcaccgcact cgtcccgacc cccggtgatg gcaccgccac caccgcagcc gccgaaggct
    11461 tcttcgaggc cgccctcgcg gcgaccacca ctccgctcag gtccaattca gcggcctcct
    11521 tggtccccgt gagcttcgcg gttggcacga ccgacccctc cagcgggccc gacgagcagc
    11581 tcgccagtct cgacaaggcg acgtcactgg ccgcggacct ggcgcagctg cacgccaagc
    11641 aggcgcgcga gcaggccgac cgcgaccgcg tccaggccgt gatcgacagc ggcggcctgg
    11701 acgggtggat cgccgaggcg ctgcagatca tggacatgcc gcagtcctac gcctccggcc
    11761 tcaagaggat catcatggcc gagtcgggcg ggaacccgcg ggccatcaac acctgggaca
    11821 gcaatgcccg ccgcggcaca ccgtcgcagg gcctgatgca gacgatccca tccacgttcc
    11881 gggcatatgt gcatccgtcg ctggccggcc ggcccatcac cgaccccgtc gccaacatca
    11941 ccgccggggt gcggtacatg atcgcgaact acgggatcgg gaccctcaag gccggcggac
    12001 gcagcaacag ctccggcgac tacgtcggat actgagctca caacctcgga gtcagcccgc
    12061 cgcacaactg cgcgcgcagc gcccgagccg tacacgtgat ccgcggagcc gacggggacc
    12121 gccgggcctc ctcagcggcc gacgaggtta gacgagcggg accggccgag cgcgccggtc
    12181 gccgatcgcg acgacatcgc cggctcggag ctgcgtgccc ctccgggtct cgacccgccc
    12241 gttcaccagc acctcttcgg cctccagcag cgccttcgcg tgtcccccat gctcggcgag
    12301 tcccgcgagc ttgaggaact ggccgagtcg aatggggtcc tcggagatcg ggacatcgag
    12361 gatcggagat ctcggcacgc acccatcatg gcgcacaccc cgcttcgcca ccccgtcggc
    12421 gcggtggccc gacgagcgcc tccggacctc ttcggggtgt gagtcaagtg agcacgacga
    12481 cccggcggga gaaccgtgcc ctacgtcgga agcccgaagt aggcacgcat cgtggtgccg
    12541 tcggggccgg tgtggagccg gacgaggtcc gcgagatggt tgaccaggaa caaccctcgc
    12601 ccgcgggcct gcagggggcg gagcggacgt cggccgagga ggggatcgtg cagttgcccc
    12661 acgtcccgga cctcgacgac gaggtgcgca tcctcgatcc agatgcggag gatgcccatc
    12721 ccgccgccgt gctcgacgct gttggtgacc agttcgttga ccaccatctc gacgtcggcg
    12781 acccggtcct ccccgaggcc tgcgcggccg gcctccgcgt gggcgaaccc gcgagcccgg
    12841 ctcagcatcg actcgtcgac gcgcaacggc gggtgctccg tgagctccgg aagagggcgg
    12901 ttgcactcgg cgagaacccg atcgaccgcg tacgacgtgc tggccctcgg tgcgccggcg
    12961 tccagcacca tcggatgcgt cgcctccgcg tcggccagca cctccggcgg caggccctcg
    13021 gcgtcgtaag ggcacaggat cgtggccggg cgaccggcga acgccaggtt gatcagggcc
    13081 tcgtgctgga cgcacgccgg gtactcagtc gcgctgcggc caggccagat cggctcaccg
    13141 atgatgcgca cccgggccgc atccgggtgc tcgtccgcga aggcgagcag cactcccggc
    13201 aggatccggc cggggttgcg tcccgcctcg ttcatgtcca ggaaccgcac ctggtctgcg
    13261 acggaaccaa gctcggcgcg caacggctcg agccgggagc cgggtacagc caccgcgacc
    13321 ggttcgtcgg cgcccaggcc ttcgtggatg aacgacagcg tgccggcaag gtagtcgtcc
    13381 agcccggtgt agaacagcgc cgggtgctcg aactgcctct cccgcgatgg catcggatcg
    13441 atcgcgaacg ccccactcac gatgccacct ccagctcccc agcgacctac ggatgcatcg
    13501 tgaaccgcag ccacacgagg gaccggtcaa ctagacgcgt ggccgcgtcc cactgacatt
    13561 gttgcagcac gccgaccgcg gaagcggcac agccaccgcc gcggaggacg agaacggtcg
    13621 ccatcagacg atgcgtaacc ggatgttggt cgccaacggc gcgtgggtcg ggctgagccg
    13681 ttcgagcttg acgttggtcc caccctggtg atcgaacagt cggacgccgt cgcagagcag
    13741 gacgggcgcg atgcgcacga agatctcatc gagcacaccc gcgtcgaggg actgccgggc
    13801 gatcctggcg ccgaggacgt tgacgtacgt gtcacccgcg gcggcctgcg ccgcggcgac
    13861 gccgctgtcg aggtcgcgac gaaggtgacc ggaatcgtcc cctatgcagg ccgatccatc
    13921 cgttctacgc gggctcgcaa ccgcccctcc ggcgccgtcc acgtgatctc atcccccggc
    13981 tgcgccccga gcagcgcccg ccccagcggg ctctcgggcg tgacgaccgg gacgtcctca
    14041 tcagggaacc gcgtgacctg cgcggactcg gaggagccgt cagggaaccg gagcgtcacg
    14101 acgctgtagg gcccgacccg ctcggacgtg tccccttcgg gtcgtccggc ctcccagagg
    14161 tagtgcacga cgtcacggat gcgccggtcg atccagtcga tctcgtcgcg gagctcggtg
    14221 gtcaggccct cctcgccgaa gtcgtgcggc cgatggtcgt cggccacgag actctcctcc
    14281 agctcgcgcc gacgcgcccg cagcagcccc agctcctcca gcagagcatg gcgctcgtcg
    14341 tgagcggcgg ccgtcatacc tcaccgtcgc acgcggctcc gccgctgcgt ccggcccgcg
    14401 agggcggact tgtcccgtgc ccttcgcccc cacgggcgga ggaggccgaa cgcgggaggc
    14461 ggtgctgatc cggctgcatg tcgtcagagc cgctgagctt cttccccgtt cgcccggaca
    14521 cgagggaccc ctgccttcgg cagccgagcc ggccacgttg tgtgctcccc agccgaacat
    14581 gcggtcgagg gccgacgcct gatcttgatc aattccagaa gatgggccac acttcggagc
    14641 gtgccgacca cttcggactt cgagcgcatg ttgcgcgggg ttgctctgcg tgtgacgcgt
    14701 cctcgggtag cggtgctctc cgcggtgcac gaccatccgc acgccgacac ggcctcgatc
    14761 atcggtgtcg tgcgtgagga tctcggtgag gtgtcccacc aggccgtcta cgacgtgctg
    14821 cgcgcgctga ctgccgcggg cctaattcgg tgcatccagc cgccgggctc cttggcgcgc
    14881 tacgagtcgc gagtccggga caaccaccac cacgtcgtgt gtcggtcgtg cggtgccatc
    14941 gccgatgtcg attgcgccgt cggcaccacg ccctgcttga ccgcatccca cgaccacggc
    15001 ttcgcgatcg acgaggccga ggtcatctac tggggcctgt gcccggactg ctcccccgca
    15061 gtcagttcct gagcacaccc ttgatcccgg aaggattccc gtgtctgaca gtccaaacgc
    15121 cgtcgtcgga gagatgaacg aggagcagga agcccggtgc ccggtcgcga ccgggcgccg
    15181 cccgcacccg accgagggcg ggagcaatcg agactggtgg ccgaaccagc tcaacctgaa
    15241 gatcctgcgg aagcaccccg ccgtggccga ccccatgggc gaggacttcg actacgccgc
    15301 ggcgttcaac acggtcgacc tcgatgccct ggccagggac gtagacgagg tgctgacgac
    15361 gtcgcaggag tggtggccgg ccgacttcgg ccactacggg ccgttcatga tccggatggc
    15421 gtggcacagc gccggcacgt accgcatcca cgacggccgc ggcggcgcag gcgcaggcat
    15481 gcagcgcttc gcgccgctga acagctggcc cgacaacggg aacctcgaca aggcgcgccg
    15541 gctgctctgg ccggtgaaga agaagtacgg ccgcaaggtc tcgtgggccg acctgatgat
    15601 cttcgcgggt aaccgcgccc tggagacgat gggcttcacg accttcggct tcgccggcgg
    15661 ccgggcggac gtctgggaac ccgacgagga cgtctactgg ggccccgagc gcacctggct
    15721 cggcgacgag cggtacaccg gtgaccggga gctggagaac ccgctggccg ccgtccagat
    15781 gggcctcatc tacgtcaatc ccgagggccc gaacggcact ccggacccgc tcgcggcggc
    15841 ccgggacatc cgcgagacct tcgggcggat ggcgatgaac gacgaggaga ccgtcgcgct
    15901 gatcgcgggc gggcacacct tcggcaaggc gcacggcgcg gccgacccgg acaggtacgt
    15961 cggcgccgag cccgagggcg ccccgctgga gctgcagggt ctgggctggg cgagctcgtt
    16021 caacagcggc aagggcgagg acgccatcac cagcggcctc gaggtgacct ggaccgaccg
    16081 gccgaccgag tgggacaacg gcttcttcga catcctcttc ggctacgagt gggagctgac
    16141 caagagcccc gccggtgccc accagtggaa gccgaagaac gacggcggcg cgaacaccgt
    16201 gcccgacccc gaatccggtg agctcgtccg cacgccgacg atgctgacga c
//
LOCUS       DASRSC010000192         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4164235,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000192 DASRSC010000000
VERSION     DASRSC010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4164235"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1067..2404
                     /locus_tag="VFE39_09520"
     CDS             1067..2404
                     /locus_tag="VFE39_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET6258877.1"
                     /translation="MTDRRTAPASESTRCYVLDTSVLLSDPWSLTRFDEHEVVLPLVV
                     ISELEGKRHHPELGWFARTALRTLDELRIEHGRLDAPVPIGTAGGTLHVELNHTDPEV
                     LPAGFRTDSNDSRILACALNLAAERKLDGRGGVVLVTKDMPLRVKAAAVGLQADEYHA
                     LDVVPSGWTGMADLEVTSADVDALFRDGAIDHDAARDLPCNTGLRLTGGGSAALGRVT
                     ADKRVRLIRGDREAFGLHGRSAEQRVALDLLLDPEVGIVSLGGRAGTGKSALALCAGL
                     EAVLERQQHRKVVVFRPLYAVGGQELGYLPGSESEKMSPWAQAVFDTLGALVSQPVMD
                     EIMARGMLEVLPLTHIRGRSLHDTFVIVDEAQSLERNVLLTVLSRLGTASRVVLTHDV
                     AQRDNLRVGRHDGVAAVIEKLKGHPLFAHVTLTRSERSPIAALVTEMLEGPAA"
BASE COUNT          349 a          825 c          856 g          385 t
ORIGIN      
        1 ccgcgagcag cccggcgacg gacgccgtga cgataattcc agccttcttc agcacagttt
       61 ctctcctgtt ggctcgtgcg gagtcgaccg gagccgattc catttagtgt cagcgcttga
      121 ccggggagcc ctcgctgacc gagagaaagg tagcgcgtcg gggatggcgc aggcaaacga
      181 agtaccgcca tcgggtgagt caaatacttt gcgtttggtt acgcatcctt tcgggggtct
      241 tcgcgaccag tgcaacgcaa tggacgggca tgcacatcca cgttccgtga ttatgaatca
      301 cccctctgcg cgcgtatcct tttcgatgga gacgcagcac gtgatcggtg gtgtccggca
      361 tgtgggcgcc cgagcccgcg gcgggttgcg ccggcgcggg gcgagaggtg gtcgccgtcc
      421 acctgctcat cgccctgagc atcaccgaat gctcccccgg gtcgtcacca gggacggctg
      481 tgacgccgcc tgcgtggtcg ggtgcgccgt agcccgctgc cgctgccggc ggttgctcgc
      541 cgacctctct tgcggggccg gcgccacgcc gctacggagg agcggttccg gcgcctcctc
      601 gacgaggctt gcgccagtgg ccgtgcgaga ggtccgctgg cccacgtggc ggaacgggca
      661 aatggtgcaa tagcccacga cggacggtgt tcgtcgagcg gtgaacggta gtccgttctg
      721 ttaattctcg gtgctggtgt accagatgac acggcgtggc tgttgaccgc acccgggtaa
      781 ccgccggtag cgttctcctc ggcggtgcgt acccgatgcg gaccgctcgg gaggccctgg
      841 tcgtggtgtc acttgcaccc gagggggccg gcatccggcc ctcgcgggcg tggacggcgg
      901 cgagttccag gtcgtcggaa cgcccaagcc agggtcggcc ggcccaccag tgtgtgggcg
      961 cggtgccgcg cccacgaggc agcattcgtc gcaccagtcc acgagggcgc atagaggcgc
     1021 tccacctcgc gggaacggcg tcgacgagcg ctagcgagga gcatccgtga ccgaccgtcg
     1081 taccgccccc gcttcggaat ccacgcgctg ctacgtgctc gacacctctg tcctgctctc
     1141 cgacccgtgg tcgctgaccc ggttcgacga gcacgaggtc gtcctgccac tggtcgtcat
     1201 ctccgaactg gagggcaagc ggcaccaccc ggagctgggg tggttcgccc gcacggcact
     1261 gcgcacgctt gacgagctgc gcatcgaaca cggccgcctg gacgcgccgg tgccgatcgg
     1321 caccgcaggc ggaacgctgc acgtcgagct caaccacacc gacccggagg tgctccccgc
     1381 tggtttccgc accgactcga acgacagccg catcctggcc tgcgccctca acctcgccgc
     1441 cgaacgcaag ctcgacggcc gcggcggcgt cgtcctcgtc accaaggaca tgccgctgcg
     1501 ggtgaaggcc gccgctgtcg ggctgcaggc cgacgagtac cacgccctcg acgtggtgcc
     1561 gtccggctgg accggcatgg ccgacctcga ggtgacgtcc gcggacgtgg atgcactgtt
     1621 ccgggacggc gccatcgacc acgacgcagc ccgtgacctg ccgtgcaaca ccggcttgcg
     1681 gctcacgggc ggcgggtcgg ctgccttggg ccgggtgacg gccgacaagc gcgtccggct
     1741 catccgcggc gaccgcgagg cattcgggct gcacgggcgc tccgccgagc agcgggtcgc
     1801 gctggacctg ctgctcgacc cggaggtcgg gatcgtgtcc ctcggtggcc gggccggcac
     1861 cgggaagtcg gcgctcgcgc tctgcgccgg cctggaggcg gtgctcgagc gtcagcagca
     1921 ccggaaggtg gtggtgttcc ggccgctcta cgccgtcggc gggcaggagc tcgggtacct
     1981 gcccggcagt gagagcgaga agatgtcgcc gtgggcgcag gcggtgttcg acacgctcgg
     2041 cgcgctggtc agccagcccg tgatggacga aatcatggcg cgcgggatgc tcgaggtgct
     2101 gccgctcaca cacattcgcg ggcggtcgct gcatgacacc ttcgtgatcg tcgacgaggc
     2161 acagtcgctg gagcgcaatg tgctcctcac cgtgctctcc cggctgggaa cggcgtcccg
     2221 ggtggtgctc acccatgacg tcgcgcagcg cgacaacctc cgcgtggggc ggcacgacgg
     2281 cgtcgctgcg gtgatcgaga agctgaaggg ccacccgctc ttcgcgcacg tgacgctcac
     2341 ccgcagcgag cggagcccga tcgccgccct ggtgacggag atgctggagg gcccggccgc
     2401 ctgaacgggt cgtgc
//
LOCUS       DASRSC010000193         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3345650,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000193 DASRSC010000000
VERSION     DASRSC010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3345650"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /gene="prs"
                     /locus_tag="VFE39_09525"
     CDS             complement(<1..423)
                     /gene="prs"
                     /locus_tag="VFE39_09525"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET6258878.1"
                     /translation="MSSTTTSGTKKMLLVSGRAYPALADEVASCLGIKPTPTKLFGFA
                     NGEIFVRYLDSVRGCDVFALQSHTSPINEWIMEQLIMVDALKRASASRITVVTPFFGY
                     ARQDKKNRGREPISARLMADLFATAGADRLMAVDLHTAQ"
     gene            complement(420..1961)
                     /gene="glmU"
                     /locus_tag="VFE39_09530"
     CDS             complement(420..1961)
                     /gene="glmU"
                     /locus_tag="VFE39_09530"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET6258879.1"
                     /translation="MSEPRPVAVIVLAAGEGTRMNSRIPKVLHSMCGRSMLGHALAAA
                     GELAPERLVVVVGHRRDEVSAEAARQAPDVHVVVQDRLGGTGHAVRTVTEALGDLSGI
                     VVVTYGDMPLLRAQTLAALVREHAAAGNAVTVLTALVPDPSGYGRILRDGSGTLAAIV
                     EEADATAAQRAISEINSGCYAFDGALLADAIKRVATGNAQGQEYLTDVVGILRGDGHL
                     AGTVLAGDPAEIQGVNDQVQLAQARRAFNGRLLEAWMRAGVTIIDPSSTWVDVDVTLA
                     SDAMILPGTHLEGRTSVGTGARIGPGCLLRDTSVAPDAMLLYAVCESAEIGPGATVGP
                     FARLRPGTRIGPGAHIGTFVELKNSTVGEGAKVPHLSYVGDADIGERSNIGAATIFAN
                     YDGMAKHHTTVGAHVFTGSDTVLVAPLTIGDGAYTAAGSVVTEDVPAGAIAVARGRQH
                     NSPGWVERKRPGTGSAAAAARARQAGAPGTELPAAGAPGTGAPGTGAPAGVTDAEGHE
                     GARDR"
BASE COUNT          261 a          740 c          726 g          301 t
ORIGIN      
        1 ctgggcggtg tgcaggtcga ccgccatcag ccggtcggcg cccgcggtgg cgaacaggtc
       61 ggccatgagc cgggccgaga tcggctcgcg gccccggttc ttcttgtcct gccgcgcgta
      121 cccgaagaac ggcgtcacca cggtgatccg gctggccgac gcccgcttca gggcgtcgac
      181 catgatcagc tgctccatga tccattcgtt gatgggcgac gtgtggctct gcagcgcgaa
      241 cacgtcacag ccccggacgg aatcaaggta gcggacgaag atctcgccgt tggcgaagcc
      301 gaagagcttg gtcggcgtcg gcttgatgcc caggcacgag gccacctcgt cggccagcgc
      361 gggataagcg cggccggaca cgagcagcat cttcttcgtg cccgacgtgg tcgtgctgct
      421 caccggtccc gtgctccttc gtgcccctcg gcgtccgtga cgcctgctgg tgccccggtt
      481 cctggtgccc cggttcctgg tgctccggcc gccgggagct cagtccccgg tgccccggcc
      541 tggcgggcgc gggcggcggc cgcggcggat ccggtgcccg ggcgcttgcg ctccacccat
      601 cccggcgagt tgtgctgcct gccgcgggcg acggcgatcg ccccggcggg cacgtcctcg
      661 gtgaccaccg agccggccgc ggtgtacgcc ccgtccccga tggtcagggg ggccaccagg
      721 acggtgtcgc tgccggtgaa cacgtgcgcg ccgaccgtcg tgtggtgctt ggccattccg
      781 tcgtagttgg cgaagatcgt ggccgcgccg atgttggagc gctcgccgat gtccgcgtcg
      841 cccacatagg acagatgggg caccttggct ccctcgccca ccgtggagtt cttcagctcg
      901 acgaaggtgc cgatatgcgc gcccgggccg atccgggtac cggggcgcag ccgggcgaac
      961 ggcccgacgg tcgcgccggg gccgatctcg gccgactcgc acacggcgta gaggagcatc
     1021 gcgtccgggg ccaccgaggt gtcccgcagc aggcagccgg ggccgatccg ggcccccgtc
     1081 ccgaccgacg tgcgtccctc caggtgcgtg cccggcaaga tcatcgcgtc ggacgcgagc
     1141 gtcacgtcca cgtcgaccca ggtcgacgac ggatcaatga tcgtgacccc ggcgcgcatc
     1201 cacgcctcga gcaggcggcc gttgaaggcg cggcgggcct gggcgagctg cacctggtcg
     1261 ttgacgccct ggatctcggc cggatcgccg gccaggacgg tgccggccag gtggccgtcg
     1321 ccccgcagga tgcccacgac gtcggtcagg tactcctggc cctgcgcgtt gccggtggcg
     1381 acccgcttga tcgcgtcggc cagcagcgcg ccgtcaaagg catagcagcc ggaattgatc
     1441 tcgctgatgg cccgctgggc cgcggtggcg tcggcttcct ccacgatcgc ggccagcgtg
     1501 ccgctgccgt cccggaggat gcgcccgtac ccggacgggt ccgggaccag cgcggtgagc
     1561 acggtcaccg cgttacccgc cgcggcgtgc tcccggacga gcgcggccag cgtctgcgcc
     1621 cggagcaggg gcatgtcacc gtaggtcacc accacgatgc cggacaggtc accgagagcc
     1681 tcggtcaccg tgcggaccgc gtgtccggtg ccgcccagcc ggtcctgcac caccacgtgc
     1741 acatcaggcg cctgccgggc ggcttcggcg ctgacctcgt cccggcggtg gccgacgacg
     1801 accacgaggc gctccggcgc gagctcaccc gccgccgcta gcgcgtgccc gagcatgctg
     1861 cgaccgcaca tggagtgcag caccttgggg atccgcgaat tcatccgggt gccctcgccc
     1921 gcggcgagga cgatgacagc gaccgggcga ggctcgctca cggcagcagg tctcctcggt
     1981 tagcgcacca gcatctgcgg tctccggggc ttccgcgggt ttcgcccg
//
LOCUS       DASRSC010000194         8440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5476515,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000194 DASRSC010000000
VERSION     DASRSC010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8440
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5476515"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..786
                     /locus_tag="VFE39_09535"
     CDS             160..786
                     /locus_tag="VFE39_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET6258880.1"
                     /translation="MSTNTSPDTRTYGRSPDGDPPVAGASQPRLHQHGPEIQPFRTMR
                     QLPLALSFETRAQSVQLLNRILADSQILHSHYKKYHWLMRGPTFYQLHLLTDKHAAEQ
                     ATLVDELAERIQSLGGVSVGDPRHAAEITELPRPPDGAQAVPSMLSQLLEDHEIIIGV
                     LRDAIQKTEQNGDSGTNDLLTGTVLRCHEMQVWFLAEHLVETPLVKEE"
     gene            950..1399
                     /locus_tag="VFE39_09540"
     CDS             950..1399
                     /locus_tag="VFE39_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6258881.1"
                     /translation="MSLMSDPFAGFERLTQQMMGGRQRPHPIPMSAVRRGDQVIAYLD
                     LPGVGHEDVDITIERNVVTIRAERRPNYQDGDEVIVDERPYGVFTRQVFLGDSLDLDK
                     MTADYARGELCLTIPISEKAKPRRIELHSSEGEPQQVTAGGTEGSAS"
     gene            complement(1533..2207)
                     /locus_tag="VFE39_09545"
     CDS             complement(1533..2207)
                     /locus_tag="VFE39_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6258882.1"
                     /translation="MRAAPQSRSFEVLDVDLDGAVALPPDPAGVVLFAHGLGGSRHDE
                     RDVLAARELNNIGIATVMVDLLTPAEQRIDAATAEFRFDAAFLGVRVIALADLLAAER
                     GTSGLPGGVLGSHSGAAAALIAAAARPQWVRAVASRGGRPELAGEFLQLARAPTLLVV
                     GEYDHPLRELNEEAADRIAGPSRVAVVPDAGHLFDEPGAAAMAAELTRDFFRVLLRAR
                     EQAYSN"
     gene            2344..3117
                     /locus_tag="VFE39_09550"
     CDS             2344..3117
                     /locus_tag="VFE39_09550"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HET6258883.1"
                     /translation="MDDDRFLSIVEREAGVDREAAVDAVRVTLTTLAQRVKPGPLREV
                     QRQAPELVRPLLSPKQPRELYDAAGFVARVAERLGVDEESARRRARAVFVALSRAVSL
                     EAWQDVVRELPMDYAPLLAGSLRSRVPMPLAEFLDWVTDRGGIDRETARRASEAVLET
                     LGERITGGEVDDVLKELTPELRPPLERGRERSRGRAQKMSLEEFVGRIAEREGVPVEE
                     ARAHARAVMNTIRDAVVAKEWRDISAQLPNSYLEELAEP"
     gene            complement(3090..3611)
                     /locus_tag="VFE39_09555"
     CDS             complement(3090..3611)
                     /locus_tag="VFE39_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6258884.1"
                     /translation="MVIAFDGSPAALRALWEAAALLAPRPGLVVVVWEAGVAYDFATI
                     PSAGLDLPPAQFDLRSAAELDQALYADARELARHGAAIATSLGLPSEGLAVADELTVG
                     ETLVRVADDVDAPAMVVGRHEHSALHDLLLGSTARHVIQHVPCPVLVVRHAEKKGDST
                     PRGRGYGSASSSR"
     gene            3870..4796
                     /locus_tag="VFE39_09560"
     CDS             3870..4796
                     /locus_tag="VFE39_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6258885.1"
                     /translation="MTGTPDDVADGGTPPPRWFVESLAAPVERGEAEVDGVAIRYRAW
                     GERGGDGLVLVHGGAAHGRWWDHVAPLLAARGTRVVAPDLSGHGDSGRRASYGLASWA
                     DEVLAIADVAGAGEAPVVIGHSMGGMVALTAALRFGARLGGVMAIDAPVRDHAPEEVA
                     AREQRAFGPLRTYPTREAAISHFRTVPLQPDDLPYVRAHIASTSVRAVDGGWAWKFDP
                     RVFGRIGLTPGQLDRVPCRVALFRAEHGLVPAAMGEMIFDRMGRVIPVVEIPAAGHHV
                     MIDQPLALVTALRTILADWRHSLPLVSAAPVA"
     gene            complement(4818..6455)
                     /locus_tag="VFE39_09565"
     CDS             complement(4818..6455)
                     /locus_tag="VFE39_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET6258886.1"
                     /translation="MATSTDGVPGAQPTAPRPGPAEPSSPEESGRMTHRQTLEALSGM
                     LLAMFTAFLSSTIVSNALPTIITDLHGTQHQYTWVITATLLSSTASTPIWGKLADLFS
                     KKLLVQLSILIFTVGSVLAGLAQSVDTLIAWRAVQGLGLGGLQSLVIIAIAAMISPRE
                     RGRYTGPIAAVMSVATVAGPLIGGVIVDTSWLGWRWCFFVGAPLAVVSLVVVQRTLNI
                     PVIRRPVHIDYLGAALIAGGVCDLLIWVTLAGTEFAWASSTSLLLVAIGVVLLGGAVL
                     VETMAKEPIIPLRLFRDRTTTLATLGSIAVGVALFGGAVFLGQYFQIARGYSPTSAGL
                     LTLPMIIGSMASSTLSGLLITRLGRWKGFLVGGSVLMVAGFALLATTDHATNIVLLGV
                     YLFLLGTGMGMTMQNLVLAVQNTVAGTDLGAATSTVSFFRSLGGTVGVSVLGAVLAAR
                     VTDLTMRGITAAGVPVPAGSDASSVGIGSLNELPDALADIVRAAYGDATAQVFLVAAV
                     LSVLTLLAVLAIKEVPLRTKSGLEMQREAMQEAGETA"
     gene            complement(6508..7017)
                     /locus_tag="VFE39_09570"
     CDS             complement(6508..7017)
                     /locus_tag="VFE39_09570"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET6258887.1"
                     /translation="MYQPVGGPVIASTTAADLVDALRTIVRTGRATRQAAHFGELPGS
                     SAATLVVLDRHGEQRMGELAERLCVDPSVVSRQVATLTRSGFAARRPDPVDRRAQLLG
                     ITDAGREALQRYRGEWVAWVAEALGEWNDDDAHDLVASLHRLAQNLQQMPPVPPRPPF
                     LHPTAPAVR"
     gene            complement(7057..7494)
                     /locus_tag="VFE39_09575"
     CDS             complement(7057..7494)
                     /locus_tag="VFE39_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET6258888.1"
                     /translation="MSHEDVLPALEEELATLWRRARISARDAARALHPQLDPTAYPVV
                     VLLGRTSAMRMSELGAALFLDKSTVSRQVDAAVRAGLAERTVDPSDARARLVALTPSG
                     RAKLTKLHADQRARWEDALAGWSREDIVQLTALLAKLSQAGIA"
     gene            complement(7598..>8440)
                     /gene="mftD"
                     /locus_tag="VFE39_09580"
     CDS             complement(7598..>8440)
                     /gene="mftD"
                     /locus_tag="VFE39_09580"
                     /EC_number="1.4.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235874.1"
                     /note="MftD, an enzyme found in the mycofactocin
                     biosynthesis locus, performs an oxidative deamination of
                     3-amino-5-[(p-hydroxyphenyl)methyl]-4,
                     4-dimethyl-2-pyrrolidinone (AHDP). The resulting compound,
                     now called pre-mycofactocin (PMFT), is a biologically
                     active redox cofactor that can oxidize the
                     non-exchangeable NADH of TIGR03971 family SDR-type
                     oxidoreductases; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pre-mycofactocin synthase MftD"
                     /protein_id="HET6258889.1"
                     /translation="RSVEDVCAVNDKTFFQMYWVGDRDTLAARAERAKRAGAKGLIVT
                     LDWSFSHGRDWGSPAIPEKMGWKTILKYVPEGVTRPRWGVEFLRSGKIPDLTVPNIPV
                     DGSTPPTFFGAYGQWMFTPPPSWADVAWLREQWGSDSPFCVKGITRVDDAKRAVDAGA
                     TAIQVSNHGGNNLDSTPATIRLLPAVADAVGDQIEILLDGGVRRGSDVVKALALGARA
                     VMIGRAYLWGMAAGGEKGVANVLAILRSGIDETLLGLGKSSIHQLNPGDLVIPAGFTR
                     SAQK"
BASE COUNT         1265 a         2853 c         3148 g         1174 t
ORIGIN      
        1 tacgagtgct ccagcactcg tatccgcgca cggacttccg cctgccggta ccgccggcgc
       61 ccgtggattc gccccgccag gcccatgctc tcttcgcccg caggggcgca gattgggggt
      121 cagcgggcgc gagcccgcgt ccgcgggaag gagcgttgca tgtccacgaa caccagcccg
      181 gacacccgga cctacgggcg ctcgcccgac ggcgacccgc cggtggccgg cgcgagccag
      241 cctcggctgc accagcacgg ccccgagatc caaccgttcc ggacgatgcg gcagctgccc
      301 ctcgccctgt cgttcgagac gcgcgcgcag agcgtgcagc tgctcaaccg gatcctggcc
      361 gactcgcaga ttctgcacag ccactacaag aagtaccact ggttgatgcg cggcccgacc
      421 ttctaccagc tgcatctgct gaccgacaag catgcggccg agcaggcgac gctggtcgac
      481 gagctggccg agcggatcca gagcctgggc ggggtgtccg tgggcgaccc gcggcacgcc
      541 gcggagatca cggagctgcc tcggccgccg gacggcgcac aggcggtccc gtccatgctg
      601 tcccagctgc tggaggacca cgagatcatc atcggggtgc tgcgtgacgc gatccagaag
      661 accgagcaga acggtgattc cggcacgaac gacctgctca cgggcacggt gctgcgctgc
      721 catgagatgc aggtctggtt cctcgccgag catctcgtgg agaccccact ggtgaaagag
      781 gagtgaggag tcgccgcaca gccgagcgcg gagcgactgc gggaccgccg tccgtgctgc
      841 ggacgagccc cgggcccagg gcccgggatg ccgcgatctc caggaactgg tgcataccgc
      901 cgcggtgacc ggccgccggt gaacgagcaa cgagtggagg aggaaaccga tgtcattgat
      961 gtccgatccg tttgcgggtt tcgagcggct gacgcagcag atgatggggg gccgccagcg
     1021 accccacccg attccgatgt cggccgttcg caggggagat caggtcatcg cctatctcga
     1081 cctgccgggc gtaggtcacg aggacgtcga catcaccatc gagcgcaatg tggtcacgat
     1141 ccgcgcggaa cggcgtccca actatcagga cggcgacgag gtgatcgtcg atgagcgccc
     1201 ctatggggtc ttcacgcggc aggtgttcct cggcgacagt ctggatctgg acaagatgac
     1261 agcggactac gcgcgcggcg agctgtgtct gacgatcccg atctccgaga aggccaagcc
     1321 acggcggatc gagctccaca gcagtgaagg ggaaccgcag caggtcacgg cgggcggcac
     1381 cgaaggtagc gcaagctaga cgagcatgat cgggtccgtc accggtggcc ccacgccgcc
     1441 ggtgacggac tcgtgctgcc tcggcggtgg cgatgcggcc acttccacga gatgaggcca
     1501 cccccgcgac ctgcgcggcc tgacggggac ggtcagttgc tgtacgcctg ctcccgggcc
     1561 cgcaggagca cacggaagaa gtcgcgggtc agctctgctg ccatcgccgc cgcgccgggc
     1621 tcgtcgaaca ggtggccggc gtcggggacg acggcgaccc ggctcggccc ggcgatgcgg
     1681 tcggctgcct cctcgttgag ctcgcgcagt gggtggtcgt actcgccgac gaccagcagc
     1741 gtcggggcgc gggcgagctg caggaactcg cccgcgagct cggggcggcc gccacgggag
     1801 gcgaccgccc gcacccactg cggccgtgcc gctgcggcga tcagggctgc ggcggcgccc
     1861 gagtgcgagc ccagcacccc gccgggcaag ccgctggtcc cgcgctcggc cgcgaggagg
     1921 tcggcgaggg cgatcacgcg gacgcccaga aaggcggcgt cgaagcggaa ctcggcggtc
     1981 gcggcgtcga tccgctgctc ggcgggggtg agcaggtcca ccatgactgt cgcgatcccg
     2041 atgttgttga gctcgcgcgc tgcgagtacg tcccgctcgt cgtgccggct gccgcccagc
     2101 ccgtgggcga agagcaccac accggccggg tccgggggca gcgccacggc gccgtcgaga
     2161 tcgacatcga ggacctcgaa gctccgggac tgcggggccg ccctcacggc gccgtttcct
     2221 tcacgacgcc actgtccccc gggtatccgg ggtgggcttg ccccccgcga ggtgaggatc
     2281 cgcgcaccga ttccacctgc gagtacgagc cctgtccgcc gtgcagttgg cacggtgggt
     2341 cgggtggacg atgaccgctt cctctcgatc gtcgagcgcg aggccggagt cgaccgtgaa
     2401 gccgcggtcg acgccgtccg ggtgacgctc acgaccctcg cgcagcgggt gaaaccggga
     2461 ccgctgcggg aggtccagcg gcaggctccc gaactggtgc ggcccctcct gtcaccgaag
     2521 cagccgcggg agctgtacga cgcggcgggg ttcgtggctc gggtggccga gcggttggga
     2581 gtcgacgagg agtcggcccg caggcgtgcg cgcgccgtat tcgtggcgct gagccgggcc
     2641 gtctcgctgg aggcgtggca ggacgtggtg cgggaactgc ccatggacta tgcgccgcta
     2701 ctcgcgggga gcctccgctc acgggttccg atgcccctcg cggagttcct cgactgggtg
     2761 accgaccggg gcgggatcga ccgtgagacg gcgcggcgcg cgagcgaggc ggtgctcgag
     2821 actctcggcg agcgcatcac gggcggcgag gtggacgacg tgctcaagga gctcaccccg
     2881 gagctgcggc ccccgctgga gcggggccgg gagcgcagcc gtgggcgggc gcagaagatg
     2941 tcactcgagg agttcgtggg tcgcatcgcc gagcgtgagg gtgtgcccgt cgaggaggcc
     3001 cgcgcgcacg cgcgcgcggt gatgaacacg atccgcgacg cggtcgtggc caaggagtgg
     3061 cgcgacatca gcgcccagct tccgaactcc tacctggagg agctcgcgga gccgtagccc
     3121 cggccccgag gagttgagtc gcccttcttc tcggcgtgcc gcacgacgag caccgggcac
     3181 gggacgtgct ggatcacgtg gcgcgccgtg ctccccagca acaagtcgtg cagcgcgctg
     3241 tgctcgtgcc ggccgacgac cattgcgggg gcgtcgacgt cgtccgccac ccggacgagg
     3301 gtctcgccga cggtcagctc gtcggcgacg gcgagccctt cggagggcaa tccgagcgag
     3361 gtggcgatcg ccgcgccatg ccgggccagc tcgcgggcgt cggcgtagag cgcctggtcc
     3421 agctccgcgg ccgaccgcag gtcgaactgg gcaggcggga ggtcgaggcc ggcactcggg
     3481 atcgtcgcga agtcgtaggc gacgcccgct tcccagacca cgacgacgag gccgggccgc
     3541 ggggcgagca gcgccgcggc ctcccacagg gcgcgcagcg cggcggggga gccgtcgaaa
     3601 gcgattacca ccggaccgga gggcatgggg ctggtttccc ggcagcggcc gggaggaacc
     3661 tccgcgatcc ttcggcaacg ctatcgaggc cggtccgttc cgccgctcag ctgtacgggg
     3721 acgatagttc cagccgaata aacgtcgccc cgatcgcgcg tcgaccctcc tctcgctgcc
     3781 ctgacagggc cgtcaacctg gggagggcga ccccgggccg gacggtccgc ggttacgctc
     3841 cgtgcattcc cgcacagagg agcacgtgca tgacaggtac cccggacgac gttgcggatg
     3901 gcggaacgcc tccgccgcgg tggttcgtcg agtcgcttgc ggcgccggtg gagcgtggcg
     3961 aggccgaggt cgacggggtt gcgatccgct atcgggcgtg gggtgagcgc ggcggggacg
     4021 gcctggttct ggtgcacggc ggcgccgcgc acggccggtg gtgggaccac gtcgcaccgc
     4081 tgctcgcggc ccggggcacg cgggtggtgg ctcccgacct gtccgggcac ggcgacagcg
     4141 ggcggcgcgc gtcatacggg ctggcgagct gggcggacga ggtactggcc atcgctgacg
     4201 tggcgggggc gggggaggca ccggtcgtca tcgggcacag catgggcggg atggtcgcgc
     4261 tcacggcggc gctccgcttc ggggcccggc tcggcggcgt gatggcgatc gacgcaccgg
     4321 tgcgcgatca cgcgccggag gaggtcgcgg cccgcgagca gcgggcgttc gggccgctgc
     4381 ggacctaccc gacccgcgag gcggcgatct cgcacttccg caccgtgccg ctgcagcctg
     4441 acgacctgcc gtacgtgcgg gctcacatcg cctcgacctc ggtacgagct gtcgacggcg
     4501 gctgggcgtg gaagttcgac ccgcgtgtct tcggccggat cgggctgacc cccggccagc
     4561 tggaccgtgt gccctgccgc gtcgccctct tccgcgccga gcacgggctc gtgcctgccg
     4621 ccatgggcga gatgatcttc gaccggatgg gccgcgtcat ccccgtcgtg gagatcccgg
     4681 ccgccgggca ccacgtgatg atcgatcaac cactggcgct ggtgaccgcg ctgcggacga
     4741 tcctcgccga ctggcgccac tcgctcccgc tggtctccgc tgcccccgtc gcctgaccgt
     4801 cgttcgcggg ggatcggcta ggcggtctct cctgcctcct gcatggcctc gcgctgcatc
     4861 tcgaggccgc tcttcgtccg cagggggacc tccttgatgg cgaggaccgc aagcagcgtg
     4921 agcacggaca gcacggctgc cacgaggaag acctgcgctg tggcgtcgcc gtacgcggcc
     4981 cggacgatgt cggcgagggc gtcgggcagt tcgttgaggc tcccgatccc gacgctgctc
     5041 gcgtcgctac cggccgggac cggtaccccg gccgcagtga tgccgcgcat ggtcaggtcg
     5101 gtcacacgcg cggcgagcac ggccccgagc acggacacgc cgaccgtccc gcccagcgac
     5161 cggaagaagg agacggtcga ggtggccgca ccgagatcgg tgccggccac cgtgttctgc
     5221 acggcgagca cgaggttctg catggtcatg cccattccgg taccgagcag gaagaggtac
     5281 acgccgagca ggacgatgtt cgttgcgtgg tcggtggtgg cgagcagagc gaagcccgcg
     5341 accatgagga ccgagccgcc gacgaggaag cccttccacc gtccgagcct ggtgatgagc
     5401 aggccggaca gtgtggacga tgccatcgaa ccgatgatca tcgggagcgt gagcagcccg
     5461 gccgaggtag gggagtagcc gcgggcgatc tggaagtact ggccgaggaa gacggcgccg
     5521 ccgaagagcg ccacgccgac ggcgatgctg ccgagcgtgg ccagggtggt ggtgcggtcg
     5581 cggaagagcc gcagcgggat gatcggttcc ttcgccatgg tctccacgag cacggcgccg
     5641 cccagcagga cgacgccgat cgcgacgagc agcagcgagg tgctcgacgc ccacgcgaac
     5701 tcggttcccg cgagtgtcac ccagatcagc aggtcgcaga ccccgccggc gatgagggct
     5761 gcgccgaggt agtcgatgtg gacggggcgc ctgatcacgg ggatgttgag ggtgcgctgg
     5821 acgacgacga gcgagaccac cgcgagcggt gcgccgacga agaagcacca gcgccagccg
     5881 agccaactgg tgtcgacgat gacgccgccg atgagcggtc cggccacggt ggcgaccgac
     5941 atcacggccg cgatcgggcc cgtgtaccgg ccgcgctccc gcgggctgat catggcggcg
     6001 atcgcgatga tcaccagcga ctgcagcccg ccgagcccga ggccctgcac ggcgcgccag
     6061 gcgatcagag tgtcgaccga ctgcgccaga ccggcgagca ccgagccgac cgtgaagatc
     6121 aggatggaga gctggaccag gagtttcttg ctgaacaggt cggcgagctt gccccagatc
     6181 ggggtgctgg ctgtggatga caacagggtc gcggtgatga cccaggtgta ctggtgctgc
     6241 gtcccgtgca gatcggtgat gatcgtcggc agcgcgttcg acacgatcgt cgaggacagg
     6301 aacgcggtga acatcgccag cagcatcccg gagagcgcct cgagggtctg ccggtgcgtc
     6361 atccggccgc tctcctcggg cgacgatggt tctgcagggc cgggccgtgg agcagtcggc
     6421 tgggcgccgg gcaccccgtc ggtggacgtg gccacaggtc gtcctcctcg tgcttggggt
     6481 gttcggatag cggggccggg atcggcgtca acgcaccgcg ggcgcagtgg ggtgcaagaa
     6541 cggcgggcgg ggcgggaccg gcggcatctg ctgcaggttc tgggccagtc ggtggagcga
     6601 cgcgacgagg tcgtgggcgt cgtcgtcgtt ccactccccg agcgcttccg caacccacgc
     6661 gacccactcg ccgcggtagc gctggagggc ctcgcgtccc gcgtccgtga tccccagaag
     6721 ctgtgcgcgg cggtcgacgg gatcgggacg gcgggcggcg aagcccgagc gcgtgagcgt
     6781 cgccacctgc cggctcacga ccgacgggtc gacgcacagg cgttcggcca actcccccat
     6841 gcgctgctcc ccgtgccggt cgaggacgac gagtgtggcc gcggacgagc cgggcagctc
     6901 accgaagtgc gcggcctgtc gagtggctcg tccggtccgg acgatcgtcc gcagcgcgtc
     6961 gaccagatcc gcggcagtcg tcgacgcgat cacaggcccc ccaactggtt gatacatgca
     7021 accatagcgc aaatagttgc atgtagcaag cttcagtcag gcgatgcccg cttggctgag
     7081 cttcgcgagc agggctgtca gctgcacgat gtcctcgcgg gaccagccgg ccagcgcgtc
     7141 ctcccaccgg gcccgctggt cggcatggag cttggtcagt ttcgcccggc cggacggggt
     7201 gagcgcgacg aggcgtgccc tggcgtcgct cgggtcgacg gtccgctcgg ccagaccggc
     7261 gcggaccgcc gcgtccacct gccggctgac cgtcgacttg tcgaggaaca atgcggcgcc
     7321 gagctcagac atccgcatcg ccgaggtacg gccgagcagg accacgaccg ggtacgccgt
     7381 cgggtccagc tgggggtgta gggcacgggc ggcgtcgcgc gccgagatcc gtgcccggcg
     7441 ccagagcgtc gccagctcct cctccagcgc cgggaggacg tcctcgtgtg acatggaacg
     7501 acctcctgcc atggttcgcc atgattgatg aaccacacag atgatcgcag gtgccggagc
     7561 ccgaccatcc catgtggcgg cccgaccggg ctgcctccta cttctgcgcg ctccgggtga
     7621 agccggcggg gatcaccaga tccccggggt tgagctggtg gatcgaggat ttgcccaggc
     7681 cgaggagggt ctcgtcgatg ccgctgcgca ggatggcgag aacgttggcc acccccttct
     7741 cgccgcctgc ggccatgccc cacaggtagg cgcgtccgat catcacggca cgggcgccga
     7801 gggcgagggc cttgaccacg tcgctgcccc ggcggacccc gccgtcgagg aggatctcga
     7861 tctggtcgcc gacggcgtcg gcaacggcgg gcagcagccg gatcgtcgcg ggggtggagt
     7921 cgaggttgtt gccaccgtgg ttggagacct ggatggcggt ggcgcccgcg tccacggcgc
     7981 gcttggcgtc gtcgacgcgg gtgatgccct tgacgcagaa cggcgagtcc gagccccact
     8041 gctcgcgcag ccacgcgacg tcggcccagg agggcggagg ggtgaacatc cactggccgt
     8101 aggccccgaa gaaggtcggc ggcgtggacc cgtcgacggg gatgttgggc accgtcaggt
     8161 cggggatctt gccgctgcgc aggaactcga cgccccagcg cgggcgggtc acgccctcgg
     8221 ggacgtactt gaggatcgtc ttccagccca tcttctccgg gatggccggg ctgccccagt
     8281 cgcggccgtg ggagaacgac cagtcgaggg tgacgatcag gcccttggcc ccggcgcgct
     8341 tggcgcgctc ggcgcgggcg gcgagggtgt cccggtcacc gacccagtac atctggaaga
     8401 acgtcttgtc gttgaccgcg cacacgtcct cgaccgaccg
//
LOCUS       DASRSC010000195        11160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1869469,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000195 DASRSC010000000
VERSION     DASRSC010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11160
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869469"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFE39_09585"
     CDS             <1..559
                     /locus_tag="VFE39_09585"
                     /inference="COORDINATES: protein motif:HMM:NF025382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4232 domain-containing protein"
                     /protein_id="HET6258890.1"
                     /translation="GCSGGGTQPVPVPVPVPPQVSAAPAPSNRPPQGSGPPTSGGGSA
                     NPQRCTAAELSVSVGAADPGAGSVVRPLVFTNTGSRTCELRGFPGVSYVAGADGHQVG
                     PAAEMSGERGDEVAISPRGTAVAQLQLVNVQNFEAAVCMPTSVRGLRVYAPGETAAVF
                     VPMDGTGCAGRPPGPQLTVRTVTAR"
     gene            complement(563..970)
                     /locus_tag="VFE39_09590"
     CDS             complement(563..970)
                     /locus_tag="VFE39_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 family protein"
                     /protein_id="HET6258891.1"
                     /translation="MPFDTTIAVLLRDDLETWQRLSVTAFLASGIAANPELLGQPYAD
                     ADGTPYLPMFRQPVVVLAGDAEVLAAAHNRAVRRGMPASVFTEELFATGHDEANRAAV
                     RAVARDDLKLVGLAVHGPRNAVDKVLKGATLHR"
     gene            1020..1793
                     /locus_tag="VFE39_09595"
                     /pseudo
     CDS             1020..1793
                     /locus_tag="VFE39_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643343.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(1778..2686)
                     /locus_tag="VFE39_09600"
     CDS             complement(1778..2686)
                     /locus_tag="VFE39_09600"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03564 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET6258892.1"
                     /translation="MQIGVWIEDDRPLAAVVADVEKAARLGFARAWFGQRTGWDPLTL
                     IASVGDRAPGIGLGTAVALTWPHHPLALAAQALTTQAAIGGRLTLGIGPSHQVIVESR
                     YGYPWTRPGQHVEDHLAILAPALAGEAVDVRAATVTAAGAVTVPDVARPPLLLAAHGP
                     RLLRLAGERADGVITTWSGPRGLAEHVVPQVRAAAAGRPDPQVVVGVVASLTHDPDAA
                     RAYVAENFGMAGELPSYRLSMDQQGFAGPADTVLAGDEATLEKAVRRFADAGATEFQL
                     CAVGPPAERARTVAFFGELARALPRA"
     gene            2761..3642
                     /locus_tag="VFE39_09605"
     CDS             2761..3642
                     /locus_tag="VFE39_09605"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6258893.1"
                     /translation="MGVAVEIRQLRYFVAVAEELHFGRAAARLAVVQPAVSQQIGRLE
                     RELGVRLLERSSRHVALTGDGERLLTEARSALAAVDRVGAVASELAAGRAGTLRLGTS
                     PGLGERVRRGVARMRANTPGLALVLVDGLVQAHIAALRAGELDLALVRGPVRVRGLRA
                     VELWRDRLHVVLPAAHPDAGAAAVPIGVLAEVTLRMPERSADTALHDAVLAACRAAGF
                     APSLGRPVRSIEDAMVEIGLGEPAATVVHGWAGAAGTTSDVAVRPIDPPVEVPGYLLL
                     PGDGGPDCLAALVTAFT"
     gene            3824..4867
                     /gene="mgrA"
                     /locus_tag="VFE39_09610"
     CDS             3824..4867
                     /gene="mgrA"
                     /locus_tag="VFE39_09610"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HET6258894.1"
                     /translation="MTYLAAPDRYESIDSGRTTFRRCGRSGLDLPAVSLGLWHNFGDD
                     KPLDTQRAILRRAFDRGVTHFDLANNYGPPYGSAERNFGTIFSQDFRPYRDELILSTK
                     AGYDMWPGPYGQGGGSRKYLLASLDQSLARMGVDYVDIFYSHRFDPTTPLEETMGALD
                     TAVRSGKALYAGISSYSGERTSEAVAILRELGTPLLIHQPSYSMLNRWIEDDLLDVLG
                     ETGVGCIAFSPLAQGMLTNKYLDGVPADSRAAQGKSLSPDLLTAKNITHVRALNEMAT
                     ARSQSLAQMALAWALRNPRVTSVLIGASSVTQLDDSLGALENLDFSPEELDQIDQHAV
                     EAGINLWARSSER"
     gene            complement(4941..5945)
                     /locus_tag="VFE39_09615"
     CDS             complement(4941..5945)
                     /locus_tag="VFE39_09615"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6258895.1"
                     /translation="MTDLATADAAPPSTWEQHRVELTAYCYRMLGSSADAEDAVQDTM
                     VRAWRSFDRFEGRSSVRSWLYKIATNVCLTMLDGRKRRARPMDFGPAGSAEAPLNDPS
                     PEISWLEPIPDARVLPTTADPAELAVSRESVRLAFVAALQHLPARQRAVLILREVLQW
                     KASEVAELLGTTVASVNSALQRARATLSENNLDSPAPVAELDDDHRELLSRYAQAFER
                     YDVGALVALLHEDAILNMPPYELWLQGVEQIQKFWTGHGAGCAGSRLVPLEANGLPGF
                     GQWRRNPDGSGYHAWAVQVLEISGDRISAYSSFLDVETLFPMFGLPLRLDPAGEPIPD
                     "
     gene            complement(6037..7248)
                     /locus_tag="VFE39_09620"
     CDS             complement(6037..7248)
                     /locus_tag="VFE39_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6258896.1"
                     /translation="MSAAVTTAGQRRSSRTSSWWVGVAIVLVAANLRPAVVGVAPLLS
                     EIRASEGLSGTAAGVLTALPVLCFGLLAPAAPRLARRFGIERAVLGALVLLCAGFAVR
                     AAGSVVLLFAGTLLVGAAIAIGNVLLPALIKRDFAHRTGVMTGLYTMAISAGGALAAG
                     VTVPVASAAGLDWRGALAVWGLFALIALVFWLPQLRRVDHRTGTGGMRVSGLWRDGLA
                     WQVTAFMGLQSLGYYAVSTWIPAVFADRGFDPEAAGWLLSLASFVGIAGSMAAPLLAT
                     RFSRQRLIAAGVTGITALGLLGVIALPGAELVSMALLGFGQGAALGLALTLVALRSPD
                     AAHASQLSGMAQSIGYVVAASGPFLVGAVHDATGSWTVPLVLLLLLFVPQGVVGVLAG
                     RDRRVRSGLSP"
     gene            complement(7245..7928)
                     /locus_tag="VFE39_09625"
     CDS             complement(7245..7928)
                     /locus_tag="VFE39_09625"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HET6258897.1"
                     /translation="MGLSAVRSGRVCDEVMTQLEGLIESGEWPVGSKIPAEPELVAAL
                     GVGRNTVREAVRALEHAGLLEPRRGDGTYVRATSGLRAAILRRARRSGALDVLAVRAS
                     LERDAAAFAAVRRTDADIVAIRNAVAARRASRDGGDRPAFVRADLAFHRAVIAATANP
                     VLIDLYAGLTEALERTVAQIDELDDDPAAFPGHEELAVAIIAGDPVAARAAADSYLDA
                     ARSLVEGSR"
     gene            7983..8375
                     /locus_tag="VFE39_09630"
     CDS             7983..8375
                     /locus_tag="VFE39_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HET6258898.1"
                     /translation="MRALQHKMDELDARLSRIGQHPFRAKFRLRGRDRAVVDLRGMET
                     VRRHAEDLIGKRLAPAEPRNDGKQTPYRGHPVFVAQHATATCCRTCLQRWHGIPAGRP
                     LDDAERAYVVEAICRWIAGQYPPASPPT"
     gene            complement(8707..9176)
                     /locus_tag="VFE39_09635"
                     /pseudo
     CDS             complement(8707..9176)
                     /locus_tag="VFE39_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439344.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(9266..9913)
                     /locus_tag="VFE39_09640"
     CDS             complement(9266..9913)
                     /locus_tag="VFE39_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET6258899.1"
                     /translation="MAHLLHIDSSIQGDRSVSRKLTARAAAAWRTAHPAGTVTYRDLG
                     SNPLPHLDTAGGLAGTVPPEQHTPDQAASWALTEQLVNEIKQATTLLLGLPLYNFGAP
                     SSVKTWVDRLIALGLSIDPETRTGLLGGRDFVVLAARGGGYGAGNPREGWDHAEPWLP
                     HALASTGLEPRFITAELTLATSNPAMAELIPLAEQSLAEAERTIDALWTPASAVA"
     gene            9999..10475
                     /locus_tag="VFE39_09645"
     CDS             9999..10475
                     /locus_tag="VFE39_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET6258900.1"
                     /translation="MSSWPVVNQPVHRCGALLDHLARRMRLRAEAVLAPVGLRPRHLV
                     ALTVLRARGGCPQQALAAMLEMDGTNVVGLLNELEAEKLVERRRSSEDRRRHVVELTD
                     AGAKQLAKVEIALAAVEEEVLGALDESQRETLYNLLLQAARGRVPSCTEAIDAELS"
     gene            complement(10577..10957)
                     /locus_tag="VFE39_09650"
     CDS             complement(10577..10957)
                     /locus_tag="VFE39_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258901.1"
                     /translation="MIGGRALDASAIVTFATGRSIYAAALVWTAVEEGIVLVVPSTAV
                     AAAWAQLPEKDHPVLEVLLELPVTVVDDLTATRARTVGARGGDQLQAHALLCAQERGW
                     ALVTDDADQYAGVDRAGVEVEQLP"
     gene            complement(10954..>11160)
                     /locus_tag="VFE39_09655"
     CDS             complement(10954..>11160)
                     /locus_tag="VFE39_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258902.1"
                     /translation="PDVLEEAQAAVASGAAKTLSAFVADALEAKLSRARSLAALERAL
                     GGRPPQEELDRVRRDLGLPSAPAA"
BASE COUNT         1615 a         3978 c         4015 g         1552 t
ORIGIN      
        1 gggctgttcc ggcggcggga cgcaacccgt ccccgtcccc gtgcccgtgc cgccgcaggt
       61 cagcgccgcg ccggctccct cgaaccgccc gcctcagggc agcgggcccc cgacgagcgg
      121 cggcggctcc gcgaacccgc agcgctgcac cgcggccgag ctgtcggtct cggtgggcgc
      181 agccgacccc ggtgccggtt cggtcgtacg cccgctcgtg ttcaccaaca ccggctcgcg
      241 gacgtgtgag ctgcgcggat tccccggcgt ctcctacgtc gccggcgccg acggtcatca
      301 ggtgggcccg gccgcggaga tgtcgggtga gcgcggcgat gaggtcgcca tctcgccgcg
      361 cggtaccgcg gtggcccagc tccaattggt gaacgtgcag aacttcgagg ccgccgtctg
      421 catgccgacg tcagtacgag ggctgcgcgt ctacgcgccc ggtgagaccg ccgctgtatt
      481 cgttcccatg gatggcaccg ggtgtgccgg taggccgccg ggtccgcagc tcacggtcag
      541 gacggtcacc gcccgctgac cgtcagcggt gcagcgtcgc gcccttgagg accttgtcca
      601 cggcgttgcg cggcccgtgc acggccaggc cgaccagctt caggtcgtcg cgggccacgg
      661 ctcgcaccgc cgcccggttc gcctcgtcgt ggccggtggc gaacagctcc tcggtgaaca
      721 ccgacgccgg catgccgcgt cgcacggcgc ggttgtgggc ggcggcgagc acctcggcgt
      781 cgccggcgag gacgaccacc ggctggcgga acatcggcag gtagggcgtg ccgtcggcgt
      841 ctgcgtacgg ctggccgagg agctcggggt tcgctgcgat tccgctggcg aggaacgcgg
      901 tgacgctcag ccgctgccag gtctccaggt cgtcccggag cagtacggcg atcgtggtat
      961 cgaaaggcac gaacggagcg tgccgctgtc ggctggtgcc tttcttgtac gttgttcacg
     1021 tgcctgcggt acgggcgtgg gcgccgcagg tgcccggaat cgccgaggtc ttccacgcgc
     1081 ggttcaccga ccacgcgtac cccgagcacg cccacgacac ctgggccttg ctcgtcgtcg
     1141 acgagggcgc gatccgctac gacctggacc gccacgagca cggcgccctc cgccccgggg
     1201 tcacgctgct cccgccgcac gtggcccaca cgggccgggc ggccacccac catggcttcc
     1261 gcaagcgggt gttgtacgtc gacacggagg tgctcgacga gtccctcacc gcagcggcag
     1321 tcgggatgcc gggcctggac gacggcctgc tccgcacccg cgtcgaccag ctgcatcgcg
     1381 cgctcgacac accgggctcc gcgctggagg ccgaaagcag gctcgcgctg atcggcgccc
     1441 ggatccggca gcaccttcac gcgcctgccg cagagccgcc gaccgcgcgg ctcgccgctg
     1501 acctgcgtga cctgctcgac gcccggaccg tcgcagggct cgccctccgc gatgcggcga
     1561 cgctgctgca cgcgcatccc gcccacctcg tgcgctgctt cacccaggcg tacggcctgc
     1621 cgccccaccg ctacctcacg ggccgccgcg tcgaggcggc ccgccgccgg ttgctcgacg
     1681 gggagccacc tgccgacgtc gcggtcgccg tggggttcca cgaccaggcc cacttgcacc
     1741 ggcacttcac ccgtctggtc gggaccacgc ccggccgcta cgcccgcggc agggcgcggg
     1801 ccagctcgcc gaagaacgcg accgtgcgcg cccgctccgc aggtggtccc accgcgcaga
     1861 gctggaactc cgtcgcaccc gcgtcggcga accgccgcac agccttctcc agcgttgcct
     1921 cgtcgccggc gagcacggtg tccgccgggc ctgcgaaccc ctgctggtcc atcgacagcc
     1981 ggtagctcgg tagctcaccc gccattccga agttctccgc gacgtaggcg cgggccgcat
     2041 ccgggtcgtg ggtcaggctg gcgaccaccc cgacgacgac ctgcggatcg ggccggccgg
     2101 ccgctgccgc acggacctgc ggcaccacat gttcggcgag cccacgcggg ccgctccagg
     2161 tggtgatcac gccgtccgcc cgctcgcctg cgaggcgcag caggcgtggc ccgtgcgccg
     2221 cgagcagcag cggcggccgg gccacgtcgg ggacggtcac tgcgcccgcc gccgtgacgg
     2281 tcgccgcccg gacgtcgacg gcctcgccgg cgagtgctgg cgcgaggatg gccaggtggt
     2341 cctccacgtg ctgccctggc cgcgtccacg ggtagccgta gcggctctcg acgatcacct
     2401 ggtggctcgg cccgatcccg agcgtgagcc gtccgcctat cgcggcctgt gtggtgaggg
     2461 cctgcgcggc gagcgccagc gggtggtgcg gccaggtcag cgcgaccgcc gtaccgagcc
     2521 cgatccccgg cgcacggtcg ccgaccgagg cgatcagggt gagcgggtcc cagccggtcc
     2581 gctgcccgaa ccaggcccgc gcgaacccga gccgggccgc cttctcgaca tccgcgacga
     2641 cggcggccag cggccggtcg tcctcgatcc acacaccgat ctgcatgtct ccaccccagc
     2701 gcccgaccgg gcggcgatca acgagcagct agcctgtgca gagataacgg atcgtgatgg
     2761 atgggggtcg ccgtggagat ccgtcagctc cgctacttcg tggcggtggc cgaggagctg
     2821 cacttcggcc gggcggccgc gcggctcgcc gtcgtccagc cggcggtgag ccagcagatc
     2881 ggacggctcg agcgcgagct gggcgtgcgg ctgctcgagc gctcgtcgcg ccacgtcgca
     2941 ctcactggtg acggcgagcg actgctcacc gaagcgcgct cggcgcttgc cgctgtcgac
     3001 cgggtgggtg cggtcgcgag cgagctcgcg gccgggcggg caggcaccct gcggctcggc
     3061 acaagcccgg ggctggggga gcgggtgcgc cgaggggtgg cccgtatgcg cgcgaacaca
     3121 ccgggactcg ctctcgtcct cgtcgacggg ttggtccagg cacacatcgc cgcgctgcgg
     3181 gcaggtgagc tcgacctcgc cctcgtgcga ggcccggtcc gggtgcgtgg cctgcgcgct
     3241 gtcgagctgt ggcgcgaccg gctgcacgtc gtgctgcccg ccgcgcaccc ggacgcgggc
     3301 gcagccgcgg tacccatcgg cgtgctggcc gaggtgacgc tgcggatgcc cgagcgttcc
     3361 gccgacaccg cgctgcacga tgccgtgctg gccgcgtgcc gagcggcggg gttcgcgccg
     3421 tccctcgggc ggcccgtgcg ctccatcgag gacgccatgg tcgagatcgg cctgggggag
     3481 cccgccgcta ccgtggtcca cggctgggcc ggggccgcgg gcaccacctc cgatgtcgcc
     3541 gtccgcccga tcgacccgcc ggtggaggtg cccggctacc tgctgctacc cggtgacggc
     3601 ggcccggact gcctcgcggc gctcgtcacg gcattcactt gagcagattc gctttctcgc
     3661 cgtcacagat ccgcggggtc gtgacagtgt gatccctcgt ggtcgcatta ttgccgatgt
     3721 gcagtgattg tcctttttgc acggtcggtg aagattcagg aagtttggtg gtaccgctca
     3781 cccaccctcc tgcgcgctcc ggtcggccgt gcaggatggg ggagtgacgt atctcgccgc
     3841 ccccgatcgc tacgagtcca tcgattccgg ccgcacgaca ttccggcgct gcggacgcag
     3901 cggcctcgat ctgcccgcgg tctcactcgg gctgtggcac aacttcggcg acgacaagcc
     3961 gctcgacacg cagcgcgcga tcctgcggcg cgcgttcgac cgcggcgtca cgcacttcga
     4021 cctggccaac aactacggcc cgccctacgg cagcgccgag cgcaacttcg gcacgatctt
     4081 cagccaggac ttccgtccgt accgagacga actgatcctg tccacgaagg ccggctacga
     4141 catgtggccg ggcccctacg ggcaaggcgg cgggtcgcgg aagtacctgc tggcgagcct
     4201 cgaccagtcg ctcgcccgga tgggcgtgga ctacgtggac atcttctaca gccaccgctt
     4261 cgaccccacc acgccgctcg aggagacgat gggggcgctc gacaccgcgg tccgctccgg
     4321 caaggcgctc tacgccggca tctcgtccta cagcggggag cgcaccagcg aggcggttgc
     4381 gatcctgcgt gagctcggca caccgctgct gatccaccag ccgtcgtact cgatgctcaa
     4441 ccgctggatc gaggatgacc tgctcgacgt tctcggcgag acaggtgtcg gctgcatcgc
     4501 gttctcaccg ctcgcgcagg gaatgctcac caacaagtac ctcgacggcg tccccgccga
     4561 ctcccgagcg gctcagggca agtcgctctc gcccgacctg ctcaccgcga agaacatcac
     4621 ccacgttcgc gcgctcaacg agatggcgac cgcacgcagc cagtcacttg cgcagatggc
     4681 gctggcctgg gcgctgcgca acccgcgcgt gacgtcggtg ctcatcgggg ccagtagcgt
     4741 cacccagctc gacgacagcc tgggcgcgct ggagaacctc gacttcagtc cagaggagct
     4801 ggaccagatc gaccagcacg ccgtggaggc cgggatcaac ctgtgggccc gctcgtcgga
     4861 gcgctgaccg accgtccgat gatcggttcc agtcgcacgt gaccgccccg gcgatggcgg
     4921 tgacgtgcgg ctcgcactga tcagtcgggg atgggctcgc ccgccgggtc gagccggagc
     4981 gggagcccga acatcgggaa cagcgtctcg acgtcgagaa acgagctgta cgcgctgatc
     5041 cggtcgccgg agatctccag cacctggacc gcccaggcgt ggtagccgct gccgtcgggg
     5101 ttgcgccgcc actgaccgaa gcccggcagg ccgttggcct cgagcggaac cagccgggaa
     5161 ccggcgcacc cggcgccgtg cccggtccag aacttctgga tctgctcgac accctgcagc
     5221 cacagctcgt agggcggcat gttgaggatg gcgtcctcgt gcagcaaagc gaccagcgcg
     5281 ccgacgtcgt agcgttcgaa cgcctgggcg tagcgggaga gcagctcgcg atggtcgtcg
     5341 tccagctcgg cgacgggcgc gggtgagtcg agattgttct cggacagggt ggctcgggcc
     5401 cgctggagcg cgctgttgac cgaggcgacc gtggtgccga gcagctcggc gacctcggac
     5461 gccttccact gcagcacctc gcgcaggatc aacaccgcac gctgacgggc cggcaggtgt
     5521 tgcagcgcgg ccacgaacgc gagccggacg gactcccggg acaccgccag ctcggcgggg
     5581 tcggccgtcg tgggcaggac gcgggcgtcg gggatgggtt ccagccagga gatctcgggt
     5641 gacgggtcgt tgagcggcgc ctcggccgag cccgcaggcc cgaagtccat cggacgggcg
     5701 cggcgcttgc ggccgtcgag catggtgagg cagacgttcg tcgcgatctt gtagagccag
     5761 gagcgcaccg aggagcggcc ctcgaaacgg tcgaacgacc gccacgcccg caccatcgtg
     5821 tcctgcacgg cgtcctcggc gtcggccgac gagccgagca tgcggtagca gtacgcagtg
     5881 agctccacgc gatgctgctc ccacgtgctg ggtggggcgg cgtcggcggt ggcgagatca
     5941 gtcattcggg ggtcccctct cgtggcggtg ttcacgcccg tgcggggcag cgtagggggc
     6001 agcaccgaca aatccaccca ctccgggcct gtcacctcat ggtgacaggc ccgaccgcac
     6061 ccgccggtcg cggcctgcga ggacaccgac gacgccttga ggcacgaaca ggagcagcag
     6121 cagcacgagg ggaacggtcc agctgccggt ggcgtcgtgc accgcgccga ccaggaacgg
     6181 gcctgaggcg gcgaccacgt acccgatgct ctgggccatc ccggacagct gcgacgcgtg
     6241 cgcggcgtcg ggggagcgca gggccaccag ggtcagggcg agcccgagcg cggcaccctg
     6301 tccgaagccg agcaatgcca tcgagacgag ctcggcgccc ggcagcgcga tgacgccgag
     6361 cagtccgagc gccgtgattc ccgtcacgcc ggcggcgatc agccgctgcc gcgagaagcg
     6421 ggtggccagc agcggcgccg ccatggaccc ggcgatgccg acgaagctgg ccagcgacag
     6481 cagccaaccg gccgcctccg ggtcgaaccc tcggtcggcg aacaccgcgg gaatccaggt
     6541 cgacacggcg tagtaaccga gcgactgcaa ccccatgaac gcggtgacct gccaggcgag
     6601 cccgtcgcgc cagagccccg agacgcgcat cccacccgtt ccggtgcggt ggtccacccg
     6661 tcgcagctgc ggcagccaga acaccagcgc gatcagggcg aacagccccc atacggccag
     6721 cgctccacgc cagtcgaggc cagccgcact cgccaccggc accgtgacgc ctgcggcgag
     6781 cgcaccaccg gcggagatcg ccatcgtgta cagcccggtc atcacccccg tccggtgcgc
     6841 gaagtcgcgc ttgatcaggg ccggcagcag cacgttcccg atggcgatcg cggccccgac
     6901 cagcagggtt ccggcgaaga gcagaaccac cgaaccggcg gcgcggacgg cgaaacccgc
     6961 acacagcagc accagggcgc cgagtaccgc gcgctcgatg ccgaaccgcc gggccaggcg
     7021 cggagcggcc ggcgcgagca ggccgaagca gagcaccggc aacgcggtca gtacgccagc
     7081 tgccgtgccg gacaggccct cgctcgcccg gatctccgac agcagcgggg cgacgccgac
     7141 cacagcgggc cgcaggttcg ccgcgaccag cacgatcgcg acgcccaccc accacgagga
     7201 cgtacgggac gatcggcgtt gccctgccgt ggtgacggcg gcgctcaccg acttccctcg
     7261 acgagactgc gggcggcgtc gaggtagctg tcggcggcgg cccgtgccgc gaccggatcg
     7321 cccgcgatga tcgccaccgc gagctcctcg tggccgggga aggccgccgg gtcgtcgtcg
     7381 agctcgtcga tctgggcgac ggtgcgctcc agtgcctcgg tgagacccgc gtagaggtcg
     7441 atgagcaccg ggttggcggt cgcggcgatc acggcgcggt ggaacgcgag gtcggctctc
     7501 acgaacgcgg gccggtcacc gccgtcgcgc gatgcccggc gggcagcgac ggcgttgcgg
     7561 atggcgacga tgtccgcgtc ggtgcggcgg accgcggcga acgctgcggc gtcccgctcc
     7621 aggctcgccc tgacggcgag cacatcgagc gcccccgagc gccgggcccg ccgaaggatg
     7681 gcggcgcgca ggccgctcgt tgcgcgcacg tacgtgccgt caccccgccg gggttccagc
     7741 aggccggcat gttcgagggc ccggactgcc tcccgcacgg tgttgcgccc cacgcccaga
     7801 gccgcgacca gctccggctc cgccgggatc ttcgatccca ccggccactc accgctctcg
     7861 atcaatcctt cgagctgcgt catgacctcg tcgcacaccc ggcccgaacg caccgctgat
     7921 aatcccacca agacatccta acctcccatg tttgcacgga ggcacgggaa ggctgcgacc
     7981 gcatgcgggc gttacagcac aagatggacg agttggacgc gcggctcagc cggatcggac
     8041 agcacccgtt ccgcgcgaag ttccgcctgc gtggccgcga tcgggccgtg gtggaccttc
     8101 gcggaatgga gacggtgcgc cggcacgccg aggatctgat cggcaagcgc ctcgcgcccg
     8161 ccgagccccg caacgacggc aagcagaccc cgtatcgggg ccacccggtg ttcgtcgccc
     8221 agcacgcgac ggcgacgtgc tgccggacct gcctgcaacg gtggcacggc atccctgcgg
     8281 gacgccccct cgacgacgcc gagcgcgcct acgtcgtcga ggcgatctgc cggtggatcg
     8341 ccgggcagta cccaccggca tccccgccga cctgatcgcc cgcgaatgga acggccggga
     8401 attgcgcgcc tccaccggta ccgtcaaatc agctgcccga tcacaggccc atcaccggcc
     8461 cgacacagtc ctgctggcag agatcgaact ggcaatggcc tttcgcgatt ccaggcgtcc
     8521 gacgagggtc gattcgacga tgagcccggc gagcagcgag gagaacgtgc aggggggagg
     8581 agctgccgcg tgcatcgtca gagtcagcgg cctgcccacg gccgattcgc cgcgccgggc
     8641 gacccgtccg gcagatcggc ggccgacgga gcccccggac tcagcggctc acagcagtct
     8701 ggtcggcccc acgatgggcc gcggcacagc ctgcttgact ccgccaccgt cactcaagac
     8761 gacgacgtcc ggcgcgagga tgtcgagcag gctctctggc ggaccgcggc cgggctcttg
     8821 tcgacggctt ccacgatctc gtcgtacccc agatcgaaca cctcgcgaag cacgaacacc
     8881 gcccgctcag tcggcgtgag cgtctccagc accagcagca tcgccatcga gacgctgtca
     8941 gcgagctcca cgtcctcggc cacgtcgggc gcggtcagca gcggctcggg caaccaggga
     9001 ccgatgtacg actccttgcg ccggccaagc gtacgcagcc gggcgagcgc ctgccgagcg
     9061 atgatcctta ccaggtatgc acgctgatcc cgcaccgtgt cgagatcgac gtcggcccac
     9121 cgcagccagg tctcctggag gacgtcctcc gcgtcggcgg ccgagctgag cagcaacgaa
     9181 catgatcact ccacgtggtg gaaccgaggt tctgcggggt gggcttccgg cggcacctcg
     9241 gttgaggtgc cgcctgggag atgcctcagg ccactgcgga tgccggggtc cacagagcgt
     9301 cgatggtgcg ttcggcctcg gcgaggctct gttcggccag cgggatcagc tccgccatcg
     9361 cggggttcga tgtggcgagg gtgagttcgg cggtgatgaa gcgcggttcg aggcccgtcg
     9421 aggccagggc gtgcggaagc cagggttccg cgtggtccca tccttcgcgg gggttgcccg
     9481 cgccgtaccc cccgccgcga gcggcgagca cgacgaagtc gcggccgccg agcaggccgg
     9541 tgcgggtctc gggatcgatc gacagtccca gtgcgatgag gcggtcgacc caggtcttga
     9601 cgctgctcgg tgcgccgaag ttgtagagcg gaagtccgag caggagcgtg gtggcttgct
     9661 tgatctcgtt gacgagctgt tcggtgagcg cccaggaggc ggcctggtcc ggggtgtgct
     9721 gctcgggcgg aacagtcccg gccagtccgc cggcggtgtc gaggtggggg aggggattgc
     9781 tgcccaggtc gcggtaggtg acggtgcctg cggggtgggc ggtgcgccag gcggcggcgg
     9841 cgcgggcggt gagcttgcgg ctgacggagc gatcaccctg gatgctcgag tcgatgtgca
     9901 ggaggtgcgc cacttcgtta atccttccta gttagtttgt gtaacacaga ctatctatag
     9961 cacagcgatg gatggcatgg gcgggtaggc tgtgctgcat gagtagctgg cctgtcgtca
    10021 accagccggt gcaccgctgt ggtgcgttgc tcgaccacct cgcgcgccgg atgcgtctgc
    10081 gtgccgaggc ggtgctggcg ccggtgggcc tgcggccgcg tcatctcgtg gcgctcacgg
    10141 tgttgcgggc tcggggcggc tgtccgcagc aggcgctggc cgcgatgctg gagatggatg
    10201 ggacgaacgt cgtcgggctg ctcaacgagc tcgaggcgga gaagctggtc gaacggcggc
    10261 gctcgtccga ggatcggcgg cggcacgtgg tcgagctcac tgacgcgggt gcgaagcagc
    10321 tcgcgaaggt cgagatcgcg ctggcggcgg tcgaggagga ggtgctcggc gcgctcgacg
    10381 agagtcagcg cgagacgctc tacaacctgc ttctgcaggc ggcccgtgga agggtgccga
    10441 gctgcaccga agccatcgac gccgagctca gctaggcggt atggagttcc gggttggggc
    10501 ctctccacat gtgaacgggg cggagtgcag ccaggcggcg gtcatgcagt ttctgctggc
    10561 ggcgacgatg aactctctat ggcagttgct cgacctcgac gccggcccgg tcgacaccgg
    10621 cgtactggtc ggcgtcatcg gtgaccagtg cccacccgcg ctcttgggcg catagcaggg
    10681 cgtgcgcctg cagctggtcg ccgcccctcg cgccgaccgt gcgggcgcgg gtggccgtga
    10741 ggtcgtcgac gacggtcacc gggagctcca gcaggacctc gagcaccgga tggtctttct
    10801 ccggcagctg tgcccacgcc gcggcgaccg cggtggacgg caccaccagc acgatgccct
    10861 cctcgacggc ggtccacacc agcgcggcgg cgtagatcga gcggccggtc gcgaacgtga
    10921 cgatggcgga ggcgtcgagg gcgcggccgc cgatcacgcg gcgggagcgg agggcaggcc
    10981 gaggtcgcgg cgcacgcggt cgagttcctc ctgtgggggc cggccgccga gtgcccgttc
    11041 cagtgcggca agcgagcggg cgcgggacag cttggcttcc aacgcgtcgg cgacgaacgc
    11101 ggacagggtt ttggcggcgc cgctggcgac ggcggcctgt gcctcctcga ggacgtcggg
//
LOCUS       DASRSC010000196         8399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_562580,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000196 DASRSC010000000
VERSION     DASRSC010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562580"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VFE39_09660"
     CDS             complement(<1..498)
                     /locus_tag="VFE39_09660"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HET6258903.1"
                     /translation="MWTLSGFADEISPDLDEQVRVLTELGMSQLELRGAWDTNVAEFD
                     DEQVERVARTLHGAGIGVSSIGSPIGKVPVTADLDEHVARFRRCAQVGRRLEARYVRV
                     FSFYPVDPQDPAADRDTVLRGMSALAAAAEQEGVVLLHENEKAIYGDVPERCLDLVES
                     VGSPAL"
     gene            complement(530..1732)
                     /locus_tag="VFE39_09665"
     CDS             complement(530..1732)
                     /locus_tag="VFE39_09665"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6258904.1"
                     /translation="MNDSLLDAPVRFAVVGCGVIGRLHAEVLAAEHGTALSVLVDTDP
                     AAADVVADHVAGLFGKRPAVTTSLTEALARDDVDAVAVCVPSGAHAAVSVPVLESGRH
                     LVVEKPLDVSVAAGRAVAEAAARHPGLVAAVISQHRFDPASVAVHDAVARGELGRVTS
                     AVATVSWWRSQGYYDSGDWRGTWALDGGGALMNQGVHTVDLLLWFLGRPIDVVAHTAL
                     LAHERVEVEDTVAAILRFESGALATLHATTAAYPGLTVRLQVMGDAGSAVVDNDRLYY
                     FHSAHRSTASAAGDAGVGPTRGVGGNGNQAAELLGTEETGRQVGGGPGTSAMFDPHHR
                     QYRDILEAIRAGRAPGVTVADALLALATVQAVYESARTGAPVLIADVLEGRVVPAPEP
                     PRRPAEVS"
     gene            complement(1729..2607)
                     /locus_tag="VFE39_09670"
     CDS             complement(1729..2607)
                     /locus_tag="VFE39_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6258905.1"
                     /translation="MTAAGRRRPAVDATAILPPGLRRGVIVVLVLLVLAPCAYILVAS
                     LMPDLEVASGTVVPSSVTLENWVAIWTTVPLAQGLTTSVVVCGVVAVVSALLAVTTAY
                     VLVRFTFVGRVTFLRALVGLQSVPGTLLLLPLFVVYASAPAYLGVTVVGTTWGLMITY
                     LTFALPFATWVMVTYIRGMPVELEEAGLLDGLSRVGVLRRIVLPLSAPGLIVAAIFSF
                     LLGWNDVLFASVLTQPKTRTAAVVLQAFGASQEGGALPLYGQVMSSAVVCAVPVVILY
                     LIFQRYLVGGLTGGLK"
     gene            complement(2604..3590)
                     /locus_tag="VFE39_09675"
     CDS             complement(2604..3590)
                     /locus_tag="VFE39_09675"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6258906.1"
                     /translation="MSLTSPAERPPTATGPAGDVGGPARVGTRRAGPTRRRQGPLWLM
                     TPGGALLLVVVVVPFVLALWMSVTDLDQYTLRQWAGAPFLGLGNYVEAFTSGPLLHSV
                     WLSVAFSVLTTVLTLPLGIAAAFAAHDRFRGRGLVRSVFLIPYVLPTFVVGTVWRIAL
                     GPDGAVNTVLGSVGIDGGNWLIGGRTFWTLVLVDTWAAWPFVYLLCLAGLQSIGREVH
                     EAVAVDGASWWQKTVHIVLPSLRGPLLLAVIIATLHHLNNFTLPFVLFGSPAPDEVNV
                     LPIAIYSTSFQTFRFGLAAAMSVASLVLVLIPLLFYLRAVRLDTGLEKGAQA"
     gene            complement(3587..4933)
                     /locus_tag="VFE39_09680"
     CDS             complement(3587..4933)
                     /locus_tag="VFE39_09680"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6258907.1"
                     /translation="MSARKLVAVAGALTVAVSLAACGGGSSGGGGGGHAGQTLQVYLS
                     RQASYPNEQQQWMQHVKDEFTKKTGASVEFETYSSATEEQQKIQTSVVSGQGPDIYEI
                     GTTFTPTAYSSGAFLKLGDEQWNAVGGRDRFVPSTLAMSGPDEADQIAVPFSSLPFVL
                     VYNTQMFTAAGIGGPPSTWDELVADAKKLTHGDTFGLAMDYKDPFSPWKYVWMFAQQY
                     GNPLVQGTTARIDDPTVARAYDAYFGFLTEDRIVNPDAANWASADATANFASGKSAMF
                     AMTSASVIPTLAKSPVANSYAFAPMPTVPPGESALPAGGVPATTIVSGQNLVVASYST
                     QQDLALQYVELVTSEAEQQHFSEVFGVLPTNAAAADAIASGKAQYAPVLEAGKQAKPT
                     PFTGAWSEIQLGLTNITVQSLAGLSAGSVPPDQVQRQLADLQKTAQAAVDKAAAAR"
     gene            complement(5138..5557)
                     /locus_tag="VFE39_09685"
     CDS             complement(5138..5557)
                     /locus_tag="VFE39_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6258908.1"
                     /translation="MRWVRETPAHWDADKERVLGGLAPELFGFGAPSEGDSLGDEWWH
                     VEDGDRVVGYGRLDDTWGDAEILVLVDPERRRSGVGAFILERLEQEASARHLNYIYNV
                     VPTAHPDPETVTGWLAGRGFTPNAVGELRKKVPVGAA"
     gene            complement(5554..7158)
                     /locus_tag="VFE39_09690"
     CDS             complement(5554..7158)
                     /locus_tag="VFE39_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HET6258909.1"
                     /translation="MTDLFNAAEYLTERRVAAGDGDRVALIHPGGVTTYAELSDEVHR
                     LAAGLLAIGVRPEERVLMCMADDVELATGILAAMYVGAVAVPSSTMLTGGELRKLVVD
                     SRARVLLGGAQFADAVTAAATGAPDLRQVVLTGDAAPQVVGPAALTWQDLLDAGTGTA
                     VTAPYATWDESPALWLYTSGTTGTPKGAMHRHSDIRYICETYGTQVLGVSRYDVCLSV
                     AKLFFAYGIGNSLFFPLSVGATAVLEPSRPNPKLYADRVAEHGVTLFFGGPSFWGPLM
                     AADLPLEAFATVRNGVSAGEALPARMFHGVREQYGFEILDGIGSTEALHIFISNVAGK
                     VVPGSSGFPVPGYRVELRRTDGTVIEGPGEPGMLYIAGDSICTGYWCRTEVNRAVFQG
                     EWMRTGDQYVRGEDGSYTCLGRTDDVLKVGGIWVSPTEVETRLLEHPSLAEAVVVGVP
                     DEDGLDKPVAYVVGRPGVRVDPDELIAFCRGGLASFKRPRLVVEVDELPKTATGKIQR
                     YRLRALATATEAPVPTTPADVAEVQS"
     gene            7422..>8399
                     /locus_tag="VFE39_09695"
     CDS             7422..>8399
                     /locus_tag="VFE39_09695"
                     /EC_number="1.1.99.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NDMA-dependent alcohol dehydrogenase"
                     /protein_id="HET6258910.1"
                     /translation="MKTKAAVLRGVGQPFEIEELTLDPPKAGEVLIRYVAAGLCHSDE
                     HLRHGDIVPRFPIVGGHEGAGIIEEVGAGVTRLKPGDHVICSFLPVCGHCRFCAAGMT
                     NICDMGATILDGMLPDGTYRFHSDRGEDIGGMCMIGTFSERATISENSAVKVADDVPL
                     DNAVLIGCGVPTGFGSAVHAAATEPGDTIAIYGIGGIGINAVQGAALAGAANVVAIDP
                     LANKREMAERMGATHSCPTAEEAHAVIQEITNGVGADKAIITVGVVDTAVTTNAFHAI
                     RKGGTVVITGLADPTKNTIEIPGALLTLFEKRIQGSLFGSGDPFKDIPRL"
BASE COUNT         1304 a         2982 c         2990 g         1123 t
ORIGIN      
        1 cagcgcgggc gagcccacgg actcgacgag gtcgaggcag cgctcgggga cgtccccgta
       61 gatcgccttc tcgttctcgt gcagcagcac gaccccctcc tgctcggccg ccgccgccag
      121 ggccgacatg ccgcgcagca cggtgtcccg gtccgcggca gggtcctgcg ggtccacggg
      181 gtagaaggag aagacccgca cgtaacgggc ctcgagccgg cgccccacct gcgcgcagcg
      241 gcggaagcgc gcgacgtgct catccaggtc cgcggtcacc ggcaccttgc cgatgggcga
      301 gccgatcgac gacaccccga tgcccgcgcc gtggagggtg cgggccacgc gctccacctg
      361 ctcgtcgtcg aattcggcga cgttcgtgtc ccaggcgcca cgcagctcca actgggacat
      421 tcccagctcc gtgagcacgc ggacctgctc gtccaggtcg ggggagatct cgtcggcgaa
      481 gccggacagg gtccacacgg tgggctcctc tggtcggggt gggtgggtgt cagctgactt
      541 cggcggggcg ccgaggaggt tcgggggccg gcacgacccg cccctccagg acgtcggcga
      601 tcaggaccgg ggcgccggtg cgggcggact cgtagacggc ctgcacggtg gccagggcga
      661 gcagggcgtc ggccacggtc acgccgggcg cgcggcccgc ccggatcgcc tcgaggatgt
      721 cgcggtactg gcggtggtgg gggtcgaaca tggcgctggt gcccgggccg cctccgacct
      781 gccgcccggt ctcctccgta ccgaggagct ctgccgcctg gttgccgttg ccgccgaccc
      841 cacgggtggg cccgacgccg gcgtcccccg ccgccgacgc cgtggagcgg tgagcgctgt
      901 ggaagtagta cagccggtcg ttgtccacca ccgcggagcc ggcatcgccc atcacctgca
      961 ggcggacggt caggccgggg taggccgccg tggtggcgtg cagcgtcgcg agcgcaccgg
     1021 actcgaagcg gaggatcgcc gccacggtgt cctccacctc cacccgctcg tgggcgagca
     1081 gggccgtgtg ggcgaccacg tcgatcggcc ggccgaggaa ccacagcagc aggtcgacgg
     1141 tgtgcacgcc ctggttcatc agcgcgccgc ccccgtcgag ggcccacgtg ccgcgccagt
     1201 cgccggagtc gtagtaaccc tggctgcgcc accaggacac cgtcgcgacg gccgaggtga
     1261 cccggcccag ctccccgcgg gcgacggcgt cgtggacggc gacgctcgcc gggtcgaaac
     1321 ggtgctggct gatcacggcc gcgacgaggc cggggtggcg ggcggcggcc tcggcgacgg
     1381 cgcgccccgc cgccacgctg acgtcgagcg gcttctccac caccaggtgg cgcccggact
     1441 cgagaaccgg gaccgagacc gcggcgtgcg caccgctggg cacgcagacg gcgacggcgt
     1501 cgacgtcgtc gcgggccagc gcctcggtca gagacgtggt gacggcggga cgtttgccga
     1561 acaaccctgc cacgtggtcg gcgacgacgt ccgccgcggc cgggtcggtg tccacgagca
     1621 cgctgagcgc ggtgccgtgc tcggcggcga ggacctccgc gtgcaggcgt ccgatcactc
     1681 cgcagccgac gacggcgaac cgcacgggtg catccagcag ggaatcgttc acttcagccc
     1741 tccggtcagg ccacccacga ggtagcgctg gaagatcagg tacaggatga cgacgggcac
     1801 ggcgcagacc acggcggacg acatgacctg cccgtacagc gggagcgccc caccctcctg
     1861 cgaggccccg aacgcttgca ggacgaccgc tgcggtccgg gtcttgggct gggtgaggac
     1921 gctcgcgaac agcacgtcgt tccagcccag caggaaggag aagatcgcgg cgacgatcag
     1981 tccgggggcg ctcagcggga gcacgatgcg gcggagcacc ccgacgcggc tcagcccgtc
     2041 caggagcccg gcctcctcca gctccaccgg catgccccgg atgtaggtga ccatcaccca
     2101 tgtcgcgaac ggcagcgcga acgtcaggta ggtgatcatc aggccccagg tcgtgcccac
     2161 cacggtcacg cccaggtacg cgggcgccga ggcgtagacg acgaacagcg ggagcagcag
     2221 cagcgtgccg ggcacgctct gcaggcccac cagcgcccgc aggaacgtga cccgcccgac
     2281 gaaggtgaac cggacgagga cgtacgcggt ggtcaccgcc agcagcgccg agaccacggc
     2341 gaccaccccg cagaccacca cgctggtggt caggccctgc gccagcggca ccgtcgtcca
     2401 gatcgcgacc cagttctcca gcgtcacgct gctggggacg accgtgcccg aggccacctc
     2461 gaggtccggc atcagcgagg ccaccaggat gtaggcgcac ggcgccagca cgagcagcac
     2521 cagcacgacg atcacgccgc ggcgcagccc cggcgggagg atcgcggtgg cgtccacggc
     2581 ggggcgccgg cggccggcgg cggtcacgcc tgcgccccct tctccagccc ggtgtcgagg
     2641 cgtacggccc gcaggtagaa cagcagcggg atcagcacca gcaccagcga ggccaccgac
     2701 atcgccgcgg ccaggccgaa gcggaacgtc tggaacgagg tcgagtagat cgcgatcggc
     2761 aggacgttca cctcgtcggg ggcgggcgag ccgaacagca cgaacggcag cgtgaagttg
     2821 ttgaggtggt gcagggtcgc gatgatcacg gccagcagca ggggaccgcg caggctcggc
     2881 agcacgatgt gcacggtctt ctgccaccac gaggcgccgt cgacggccac ggcctcgtgc
     2941 acctcgcggc cgatggactg caggccggcc aggcagagca ggtacacgaa cggccacgcc
     3001 gcccaggtgt cgaccagcac gagggtccag aaggtgcggc cgccgatcag ccagttgccc
     3061 ccgtcgatcc cgacggagcc gagcacggtg ttgaccgcgc cgtccggccc gagcgcgatg
     3121 cgccacacgg tgccgaccac gaacgtcggc agcacgtagg ggatgaggaa gaccgagcgt
     3181 acgaggccgc gaccccggaa ccggtcgtgc gcggcgaacg ccgcggcgat cccgagcggc
     3241 agcgtcagca ccgtggtcag cacgctgaac gcgacgctca gccacacgct gtgcagcagc
     3301 gggccggagg tgaaggcctc gacgtagttg ccgagaccga ggaacggcgc ccccgcccac
     3361 tgccgcagcg tgtactggtc gaggtcggtg accgacatcc acagcgccag cacgaacggc
     3421 acgacgacca ccacgagcag cagcgcgcca cccggcgtca tcagccacag gggcccctgc
     3481 cggcgccgcg tcgggccggc ccggcgggtg ccgacgcgcg ccggcccgcc cacgtcgccg
     3541 gccgggccgg tcgccgtggg cgggcgttcc gccgggctgg tgaggctcac cgggcagccg
     3601 ccgccttgtc gacggccgcc tgcgcggtct tctggagatc ggcgagctgt cgttggacct
     3661 ggtcgggcgg caccgagccc gcgctcagcc ccgcgagcga ctgcacggtg atgttggtca
     3721 gccccagctg gatctccgac cacgcgccgg tgaacggggt gggcttggcc tgcttgcccg
     3781 cttccagcac cggcgcgtac tgcgcctttc cgctcgcaat ggcgtcggcg gccgccgcgt
     3841 tggtcggcag caccccgaac acctcggaga agtgctgctg ctcggcctcg gaggtgacca
     3901 gctccacgta ctgcagggcc aggtcctgct gggtggagta ggaggcgacc acgaggttct
     3961 ggccggacac gatggtggtg gcgggcacgc cgcctgcggg cagtgcgctc tcaccgggcg
     4021 ggacggtggg catcggagcg aacgcgtagg agttcgccac cggcgacttc gcgagcgtcg
     4081 ggatgacgct cgcgctggtc atggcgaaca tcgcggactt cccggaggcg aagttggccg
     4141 tcgcgtcggc cgaggcccag ttggcagcgt ccgggttcac gatccggtcc tcggtgagga
     4201 acccgaagta ggcgtcgtag gcccgggcga cagtggggtc gtcgatccgg gcggtggtgc
     4261 cctgcaccag cgggttgccg tactgctggg cgaacatcca gacgtacttc cagggcgaga
     4321 aggggtcctt gtagtccatc gcgaggccga aggtgtcgcc gtgggtcagc ttcttcgcgt
     4381 ccgccaccag ctcgtcccat gtggacggtg gcccgccgat gcccgcggcg gtgaacatct
     4441 gggtgttgta gacgagcacg aacggcagcg acgagaacgg cacggcgatc tggtccgcct
     4501 cgtccgggcc ggacatcgcc agcgtggagg gcacgaaccg atcccggccc ccgacggcgt
     4561 tccactgctc gtcgccgagc ttcaggaacg ccccgctgga gtaggcggtg ggagtgaacg
     4621 tcgtgccgat ctcgtagatg tcggggccct ggcccgagac gaccgacgtc tggatcttct
     4681 gctgttcctc ggtcgccgag ctgtaggtct cgaactcgac gctcgcgccg gtcttcttcg
     4741 tgaactcgtc cttgacgtgc tgcatccact gctgctgctc gttggggtag gacgcctgcc
     4801 ggctgaggta gacctgcagc gtctgcccgg cgtggccccc gccgcccccg ccgctcgacc
     4861 cacccccgca ggctgccagg gacaccgcca ccgtgagcgc acccgccacc gctaccaact
     4921 tgcgagcgga catcgtcaga cctccttgtc tgatcgcagc agcggaccag gcctctgctg
     4981 ggcccgaaga taggcaggcc ccatcgcggg cacaatccct tgcaggaatt tgcagtgccg
     5041 ggcgcggtga ggcgctcctc atcagcagaa atgcctggac cgggcagagt cgatttgctc
     5101 ggcggcacgc agaacaggag tggcggggcc ccgggggtca ggctgccccg accggcacct
     5161 tcttgcgtag ctcgcccacc gcgttgggcg tgaacccccg gccggcgagc cagccggtca
     5221 ccgtctcggg gtccgggtgg gccgtgggca cgacgttgta gatgtagttc aggtgccggg
     5281 ccgaggcctc ctgctccagc cgctcgagaa tgaacgcgcc cactcccgac cggcgccgct
     5341 ccgggtcgac cagcacgagg atctcggcat cgccccaggt gtcgtcgagc cggccgtagc
     5401 cgaccacgcg gtcgccgtcc tccacatgcc accactcgtc gccgagggag tcgccctcac
     5461 tcggggcgcc gaagccgaac agctccgggg cgaggccgcc gagcacgcgc tccttgtcgg
     5521 cgtcccagtg cgcgggggtc tctcggaccc acctcacgac tgcacctccg cgacgtccgc
     5581 aggcgtggtg ggaacgggcg cctcggtggc ggtcgccagt gcgcgcagcc ggtagcgctg
     5641 gatcttgccg gtggccgtct tgggtagctc gtccacctcg acgacgagcc gcggccgctt
     5701 gaacgacgcg aggccccccc ggcagaacgc gatcagctcg tccgggtcga cccgcacgcc
     5761 cggccggccc acgacgtagg cgaccggctt gtccaacccg tcctcgtccg gcacgccgac
     5821 gaccacggcc tcggcgagcg acgggtgctc caacagccgc gtttccacct cggtggggct
     5881 cacccagatc ccgccgacct tgagcacgtc gtcggtccgg ccgaggcacg tgtagctgcc
     5941 gtcctcgccg cgcacgtact ggtcgccggt gcgcatccac tcgccctgga acaccgcccg
     6001 gttgacctcg gtgcggcacc agtacccggt gcagatcgag tcgcccgcga tgtagagcat
     6061 cccgggctcg ccgggcccct cgatcaccgt cccgtcggtg cgtcgcagct ccacccgata
     6121 tcccgggacg gggaacccgg agctgccagg gacgaccttg cccgccacgt tggagatgaa
     6181 gatgtgcaac gcctcggtgg aaccgatgcc gtcgaggatc tcgaagccgt actgctcgcg
     6241 gacgccgtgg aacatccgcg cagggagtgc ctcgcccgcc gagacgccgt tgcggacggt
     6301 cgcgaacgcc tccagtggca ggtcggcggc catcagcgga ccccagaagc tcgggccgcc
     6361 gaagaacagc gtgacgccgt gctcggccac gcggtcggcg tacagcttcg ggttcggccg
     6421 cgacggctcc aggaccgccg tggcgcccac cgacagcggg aagaacagcg agttgccgat
     6481 cccgtaggcg aagaacagct tggccaccga cagacagacg tcgtatcggc tgacgccgag
     6541 cacctgggtg ccgtaggtct cgcagatgta ccggatatcg gaatgccggt gcatggcgcc
     6601 cttgggggtg cccgtggtgc cggaggtgta gagccacagt gcgggcgact cgtcccacgt
     6661 cgcgtaggga gcggtgaccg cggtacccgt gcccgcgtcg agcagatcct gccaggtgag
     6721 cgcggccgga ccgacgacct gcggcgctgc gtcaccggtg agcaccacct gccgcaggtc
     6781 gggagccccc gtcgcggcgg ccgtcacggc gtcggcgaac tgggcgccgc cgagcagcac
     6841 ccgggcgcgc gagtcgacca cgagcttgcg cagctcaccg ccggtcagca tggtcgagga
     6901 cggcacggcg accgccccga cgtacatcgc ggcgaggatg ccggtggcga gctcgacatc
     6961 gtcggccatg cacatcaaca cccgctcctc ggggcgtacc ccgatcgcga ggagcccggc
     7021 ggcgagccgg tggacctcgt ccgagagctc ggcgtaggtc gtgaccccgc ccggatggat
     7081 gagcgcgacg cggtccccgt cgcccgcggc gacgcggcgt tcggtgaggt actccgcggc
     7141 gttgaagagg tcggtcatga aggtggctcc catgccggtt cgggtgggca aactctacag
     7201 cttgatcact ctccgctaaa gatcagtaga agatttgccc ggggtttgcg ttactccgat
     7261 cggccctatc cgtcgcgctt ccggccttcc caagccctgt gcaggctgct accaatgcca
     7321 agagcggtga acccgagacg atgaggtaac gggcgtcccg gggccacgcc ggagccactc
     7381 gaatcatgat cagccaatga gggctgatgt ctggagggtc catgaagacg aaggccgcgg
     7441 tgctgcgcgg ggtaggtcag ccgttcgaga tcgaggagct gaccctcgac ccgcccaagg
     7501 cgggagaggt gttgatcagg tacgtcgccg cgggactctg tcactccgac gagcacctcc
     7561 gccacggcga catcgtgccg cgcttcccga tcgtcggcgg ccacgaaggt gccgggatca
     7621 tcgaggaggt gggggccggt gtcacccggc tcaagccggg cgaccacgtc atctgctcgt
     7681 tcctgccggt ctgcggccac tgccgattct gtgccgccgg gatgaccaac atctgcgaca
     7741 tgggcgccac catcctcgac gggatgttgc ccgacggcac ctaccgcttc cattccgacc
     7801 gcggcgagga catcggcggg atgtgcatga tcggcacgtt ctccgagcgc gccacgatct
     7861 ccgagaactc cgctgtcaag gtcgccgacg acgtgccgct cgacaatgcc gtgctgatcg
     7921 gctgcggtgt gccgacgggc ttcgggtcgg cggtgcacgc cgctgcgacc gaaccgggcg
     7981 acaccatcgc gatctacggc atcggcggga tcggcatcaa cgccgtgcag ggtgcagcgt
     8041 tggccggcgc ggccaacgtc gtggccatcg acccgctggc gaacaagcgc gagatggccg
     8101 agcggatggg ggccacgcac tcctgcccca ccgcggagga ggcgcacgcg gtgatccagg
     8161 agatcaccaa cggggtgggc gccgacaagg cgatcatcac cgtcggcgtg gtcgacaccg
     8221 cggtcaccac caacgccttc cacgccatcc gcaagggcgg caccgtggtc atcaccggcc
     8281 tggccgaccc caccaagaac accatcgaga tccccggcgc gctgctcacc ctgttcgaga
     8341 agcgcatcca gggctcgctg ttcggctcgg gcgacccgtt caaggacatc ccgcggctg
//
LOCUS       DASRSC010000197         9421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_401430,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000197 DASRSC010000000
VERSION     DASRSC010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9421
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401430"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..431)
                     /locus_tag="VFE39_09700"
     CDS             complement(48..431)
                     /locus_tag="VFE39_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HET6258911.1"
                     /translation="MTDPQNFYAEVGGAPVFDKIVSRFYEQVSEDEVLRPIYPEEDLG
                     PAEVRLRMFLEQYWGGPHTYSDQRGHPRLRMRHAPFKVGPIERDAWLRCMRVAVDEAG
                     LDDEHRAQLWQYLTYAAASMVNSDF"
     gene            complement(466..1476)
                     /locus_tag="VFE39_09705"
     CDS             complement(466..1476)
                     /locus_tag="VFE39_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HET6258912.1"
                     /translation="MTTIATIPGNVAESLLPVRPACADDAGSLCAALYQWTNNDFLSR
                     SGDAILTGTLHIALIVALACAGRFLIHRAITRVVDGATNSRITQIVGRKPRLGTNGSA
                     GPVSQRRAQRARTIGSVLRSLSSAVVLLIGSIMVLGEFGVALGPILASAGIVGVAVGF
                     GAQNLVRDFLSGVFMLLEDQYGVGDIVDLGEASGVVESVGLRITTVRDVNGTVWYVRN
                     GEILRVGNKSQGYAVAVVDVPLAHSADIAQAIELVAETATERVAATDVVESVLETPEV
                     LGVERVGPEGVTLRMTVKVKPGQQFAVQRALNAAITNALDHAGVPRPGVVPPPLARPS
                     VP"
     gene            complement(1473..3575)
                     /locus_tag="VFE39_09710"
     CDS             complement(1473..3575)
                     /locus_tag="VFE39_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6258913.1"
                     /translation="MDPPRPYPDAERLDLVEDLHGHRIADPYRWLEDPEDSRTQTWST
                     AQDMLARRHLDTLPGRDRLAATLTELLSAGSVSAPLWRAGRSFFTRREPGQEHAVLHV
                     REAHGTERVLLDPTALDPSGLTTLDGWVPDLEGRQLAYQLSSGGDEQSILHVLDVNTG
                     EDIEPPIDRCRYSDVAWLPGGSEFVYVRMVDEDEAPDGEQAFHRRVWRHRVGTPAESD
                     ELVDAPGLYDEHTYYGVRISRDGTWLVVTGNVGTARRDSVWIAELGEKTPPLTPILTQ
                     DDDVQCHAWVDRDGLLYLHTTDGAPRWRLAVADPRTPGREHWHELVAEDPGSVLQAVR
                     RLEPSAPGGDTVLVLARARHAVAELALHSADGAPIGTVPLPGTGSLTGLSVADRDTPE
                     QAGRVWFGWTDLVTPPQVHRFDLATGESVLETAAPGAVTVPAVRTEHRMFTSVDGTTV
                     RMFVIAPEGRTRTRPALVTGYGGFGISREPAYTAQALAWVAAGGLYALVSLRGGGEEG
                     EDWHLAGNRANKQNVFEDLHAAAAALIDAGDTTAESLAIMGGSNGGLLVGAALTQRPK
                     LYRAVVCSAPLLDMVRYEEFSLGRTWNDEYGTAADPQELGWLLSYSPYHHVNPGVAYP
                     AVLFTVFESDTRVDPLHARKMCAALQHATTGDPLTRPVLIRRETDVGHGARSVSRTVT
                     LATDQLAFLAAQTGLELP"
     gene            complement(3726..4376)
                     /locus_tag="VFE39_09715"
     CDS             complement(3726..4376)
                     /locus_tag="VFE39_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6258914.1"
                     /translation="MSLSSTRRSTVTRLRDRARTDRADLHAVLDAGLVCHLGLVRDGV
                     PVVLPTGYGRIGDTVYVHGSTGAGYLRVADGFPVCLTVTHIDGIVYARSLFHHSMNYR
                     SAVVHGTARAVTDPAERLDALRAIVEHLSPGSWEHARQPNPRELAATAVFAIGLDEAS
                     VKVRTGPPGDDDADIEAGGRWAGVLPVHTVFGLPESCRTLPPDAPIPEHIADRRVG"
     gene            4406..5881
                     /locus_tag="VFE39_09720"
     CDS             4406..5881
                     /locus_tag="VFE39_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET6258915.1"
                     /translation="MVGPILSEFGRPLVELPPLLLDRNGERPLAVQLADALRAVTAGG
                     TLRAGERLPSTRELAATLGVSRTVTAAAYDQLLAEGWAVGRRGAGTFVVGAPAGPPAA
                     RWPSAPPEADRDGEHGAISLRAGTPCLEVLDRAAWRRAWRAAADPPPDAAPDPVGLPA
                     FRAAVVEHLLRHRGLPVDQAQVLATGGSTAAVAELARTLPAGARVAVEEPGYQRGVAA
                     LQTAGLTVARVPVDGAGLVVDALPPGLAAVYCTPAHQYPLGARLSAARRMALVERARA
                     EGFLVIEDDYDGELRYDVAPLPLLAALGPDVVAHLGTVSKLLTPTLGAGWLVVPPAVR
                     AAILDQRYRTGARPAPAGQRVFVALAAHGDLARHLRRLRRELAERRVAVAVAVRAAGH
                     ILEGDAAGAHVLVPLPGLGAEQSVVAAAAAGGVTLDGLARHHAGDPDRAGIVLGYAEP
                     RRAELERGLAVVTDVLARCTPAVTATASPVDLPGRPSRRDR"
     gene            complement(5823..8426)
                     /locus_tag="VFE39_09725"
     CDS             complement(5823..8426)
                     /locus_tag="VFE39_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6258916.1"
                     /translation="MSNTTLGLLMATINSSIVLIALPDIFRGIGLDPLASGNTSYLLW
                     MIMGFLVVTAVLVVGFGRLGDMYGRVRMYNIGFAIFTVASVFLAITWQSGSAGALWLI
                     GWRIIQGIGGAFLFANSSAILTDAFPVHQRGLALGINGVAAIAGSFIGLVLGGVLAPV
                     NWHLVFLVSVPFGVFGTFWAYFKLHDTSVRTPAKMDWWGNITFAVGLVAVLVGITYGI
                     QPYGDHVMGWTSPFVLTCIIAGIVVLVLFGIIETKVASPLFTLSLFKIRRFTLGNSAN
                     LLASLGRGGLQFVLIIWLQGIWLPLHGYSFEQTPLWAGIYMLPLTVGFLIAAPVSGVL
                     SDRIGGRGLATAGMLVSALSFVLLLILPIDFPYLGFAAVLLVNGVGMGLFTSPNRADV
                     MNSLPAGARGAGAGMTATFQNSAMVLSIGLFFGLMIAGLSEHLPTVLQQGLLEHGVPA
                     ADASRIAALPPVAVLFAAFLGYNPVQQLLGGVLDTLPPDQASFLTGRSFFPELISGPF
                     ANGLTVAFWFAICATVLAAVASWFIGGRRSTDPAASVGAELAAVGGESGFGPTEFVAS
                     IAAEREPEPGPSGPVSRVLTASAGEIIGQVRTSGGSAVPDGVVTVTDAYGRQVARSGV
                     DQDGRYALRGMPPGTYMAIATSPGFRPDVTAIVLNGSGVVHHFALSGDGTVTGAVRAA
                     SSGAPLARAMVLATDSAGRVVGSTRTGPDGAFVLPGVPAGRTTVTASLPGHVPGAAAV
                     RVVPDAPAVVEVLLQSTVAGLTGRVTGTDGAPLPSATVAVINADGDALAVTTTGADGT
                     YAITGLEPGHYTVVATAQATFPVQLPVGEQARVDLRLGSPAAQPAVVPSLFSDPGATA
                     YPADRRATLSP"
     gene            complement(8602..>9421)
                     /locus_tag="VFE39_09730"
     CDS             complement(8602..>9421)
                     /locus_tag="VFE39_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6258917.1"
                     /translation="FQHLWRQVGRNIGSYATYPRLVGETGGKDFVVAHPSADVDVLRT
                     ALIRGAFEYQGQKCSAASRAFVPRSVWTRLRDDLVDEVEALPMGDVTDFRNFLGAVID
                     RRAFDKVTAAQQAARSDPALEVIAGGTADDSTGYFVRPTVIVGSDHNHDVFRTEYFGP
                     LLAVHVYDDGDFEQVLEHVDRGAPYGLTGAVIARDRAAVARATEALRFAAGNFYVNDK
                     PTGAVVGQQPFGGGRASGTNDKAGSIYNLLRWVSPRTIKETFVPSTTTGYPHQA"
BASE COUNT         1369 a         3306 c         3486 g         1260 t
ORIGIN      
        1 atcttgccaa atccggagca gtatgagcgc gaaccgcgcg ttcggcttca gaagtcgctg
       61 ttgaccatgc ttgcggcggc gtaggtgagg tactgccaca gctgcgcgcg gtgctcgtcg
      121 tccagcccgg cctcgtcgac ggcgacccgc atgcagcgca gccacgcgtc gcgctcgatc
      181 ggcccgacct tgaacggcgc gtgccgcatc cgcagccgcg ggtgcccgcg ctggtcggag
      241 taggtgtgcg ggccgcccca gtactgctcg aggaacattc gcagccggac ctcggccgga
      301 ccgagatcct cctcggggta gatcgggcgg agcacctcgt cctcgctgac ctgctcgtag
      361 aagcgagaca cgatcttgtc gaagaccggt gcgccgccga cctcggcgta gaagttctgg
      421 ggatcggtca caggtccatc atcctccgcc ccggcgggtg cggtttcagg gcaccgacgg
      481 acgggccagg ggtgggggta ccacaccggg gcgcgggaca ccggcgtggt cgagagcgtt
      541 ggtgatcgcc gcgttcaggg cgcgctgcac agcgaactgc tggcccggct tgaccttcac
      601 cgtcatccgc agcgtcacgc cctccggtcc gacccgttcg acgcccagca cctccggcgt
      661 ctccagcacg gactcgacca cgtccgtcgc ggccacccgc tccgtggcag tctcggccac
      721 gagctcgatg gcctgggcga tgtcggcgct gtgcgcgagc ggtacgtcca cgacggcgac
      781 ggcatagccc tggctcttgt tcccgacgcg gaggatctcg ccgttgcgca cgtaccagac
      841 ggtgccgttg acgtcgcgga cggtggtgat gcgcaacccc accgactcga ccacgccgct
      901 ggcctcgccg aggtcgacga tgtcaccgac gccgtactgg tcctccagca gcatgaacac
      961 gccggagagg aagtcgcgga cgaggttctg cgcgccgaag ccgaccgcga ccccgacgat
     1021 gccggccgac gcgaggatcg ggcccagcgc caccccgaac tcccccagca ccatgatcga
     1081 cccgatcagc agcaccaccg cggagctcag cgagcgcagc accgacccga tcgtgcgggc
     1141 ccgctgcgcc cgccgctgtg acaccgggcc ggcgctgccg ttcgtgccca accgtggctt
     1201 gcggccgacg atctgggtga tccgggagtt ggttgcgccg tcgacgaccc gggtgatggc
     1261 gcggtggatc aggaaccgac cggcacacgc cagcgcgacg atcagcgcga tgtgcagtgt
     1321 cccggtgagg atcgcgtccc ccgagcggga cagaaagtcg ttgttcgtcc actggtagag
     1381 cgccgcgcac aaggagccgg cgtcatcggc gcaagccggg cgcacgggaa gcagggactc
     1441 ggcgacgttg ccgggaatgg tcgcgatcgt ggtcacggga gctccagtcc ggtctgcgca
     1501 gccaggaagg ccagctggtc ggtcgcgagc gtcacggtgc gcgagaccga ccgggcccca
     1561 tggccgacgt cggtctcgcg gcggatcagg acgggccggg tgagcgggtc gccggtggtg
     1621 gcgtgctgga gggcggcgca catcttgcgg gcgtgcaggg ggtcgaccct ggtgtcggac
     1681 tcgaacaccg tgaacagcac ggccgggtac gcgacgcccg ggttcacatg atggtagggc
     1741 gagtaggaca gcagccagcc cagctcctgc gggtcggcgg ccgtgccgta ctcgtcgttc
     1801 caggtgcggc cgagggagaa ctcctcgtag cgcaccatgt ccagcagcgg ggcggagcag
     1861 accacggccc ggtagagctt gggacgctgg gtaagcgcgg ccccgacgag caggcccccg
     1921 ttcgagcctc ccatgatcgc gagcgattcg gccgtggtgt cgcccgcgtc gatcagcgcg
     1981 gcagcggcgg catgtaggtc ctcgaacaca ttctgcttgt tcgcgcggtt gccggcgagg
     2041 tgccagtcct cgccctcctc accgcccccg cgcaacgaga ccagcgcgta cagcccgccg
     2101 gccgccaccc acgccagtgc ctgcgcggtg taggcgggct cgcggctgat tccgaacccg
     2161 ccgtagcccg tgaccagcgc cggtcgggtg cgggtgcgcc cctccggagc gatcacgaac
     2221 atccgcacgg tcgtgccgtc gactgacgtg aacatccggt gctcggtgcg cactgcgggc
     2281 acggtgaccg cgccgggggc ggcggtctcg aggacgctct cgccggtggc gaggtcgaag
     2341 cggtgcacct gcggcggggt gaccaggtcg gtccagccga accagacgcg acccgcctgc
     2401 tccggcgtgt cgcggtcggc gacggagagc ccggtgagcg atcccgtgcc gggcagcggc
     2461 acggtgccga tgggtgcccc gtcggcggag tgcagtgcca gctcggccac ggcgtgccgg
     2521 gccctggcga gcaccagcac ggtgtcgccc ccgggcgcgg acggttccag ccttcgcacc
     2581 gcctgcagta cggaccccgg gtcctcggcc accagctcat gccagtgctc ccggccgggg
     2641 gtgcgcgggt cggcgaccgc cagccgccac cgcggcgcgc cgtcggtcgt gtgcaggtag
     2701 agcagcccat cgcggtcgac ccatgcgtgg cactgcacgt cgtcgtcctg ggtgagtatc
     2761 ggcgtcaggg gcggggtctt ctccccgagc tccgcgatcc agacgctgtc gcggcgcgcc
     2821 gtgccgacgt tgcccgtgac caccagccat gtgccgtccc gggagatgcg cacgccgtag
     2881 taggtgtgct cgtcgtagag ccccggagcg tcgacgagct cgtccgactc cgcgggcgtg
     2941 cccacgcgat ggcgccacac gcgccgatgg aaggcctgct ccccgtcggg tgcctcgtcc
     3001 tcgtccacca tccggacgta gacgaactcg ctcccgccgg gcagccacgc cacgtccgag
     3061 tagcggcagc ggtcgatcgg cggctcgatg tcctcgccgg tgttgacgtc gagcacgtgc
     3121 aggatcgact gctcgtcgcc gccgctggag agctggtagg ccagctgccg gccctcgagg
     3181 tcgggcaccc agccgtcgag ggtggtgagg ccggaggggt cgagtgcggt cgggtcgagc
     3241 agcacccgct cggtgccgtg cgcctcgcga acgtggagca ccgcgtgttc ctggccgggt
     3301 tcgcgccggg tgaagaacga tcggccggcg cgccacaacg gggccgacac cgagccggcg
     3361 gagaggagct cggtcagggt cgcggcgagt cggtcgcggc cggggagagt gtcgagatgc
     3421 cggcgggcca gcatgtcctg cgccgtcgac caggtctgcg tgcgggagtc ctcggggtct
     3481 tccagccagc ggtacgggtc ggcgatgcgg tgaccgtgca ggtcctcgac gagatccagc
     3541 cgctctgcgt cggggtaggg gcggggaggg tccacgcgaa tcgaggttac gtgcaccggg
     3601 atcagtggct cccgccccct ccgcgacccg gagcgtcatc ggcgatcgcc gtcaggcgca
     3661 cctcggcggg tcggtcgtgc gcggctcgga gcgatcgcgg gggactcctc gcggccggtc
     3721 gcgattcagc ccacccgacg gtccgcgatg tgctcgggga tcggggcgtc cggcgggagc
     3781 gtgcggcacg actcaggcag gccgaagacc gtatggaccg gcaggacgcc ggcccaccgg
     3841 ccgcccgcct cgatgtcggc atcgtcgtcg cccggaggcc cggtgcgcac cttgacgctg
     3901 gcctcgtcga ggccgatcgc gaacaccgcc gtggcggcga gctcgcgggg gttcggctgc
     3961 cgcgcgtgct cccacgaccc gggggagagg tgctcgacga tggcgcgcag cgcgtcgagc
     4021 cgctccgccg ggtcggtgac ggcccgcgcg gtgccgtgca cgaccgcact gcggtagttc
     4081 atcgagtggt ggaagagcga gcgggcgtag acgattccgt cgatgtgggt caccgtcagg
     4141 cagaccggga agccgtcggc cacccgcagg tagccggcgc cggtcgagcc gtgcacgtag
     4201 accgtgtcgc cgattcgtcc gtagcccgtg ggtagcacca ccgggacgcc gtcacggacg
     4261 aggccgagat ggcacaccag cccggcgtcg agcaccgcgt gcaggtcggc gcgatcggtg
     4321 cgggcccgat cacggaggcg ggtgacggtg ctgcggcggg tggaggagag gctcacggtg
     4381 tcatcgtcgc ccggacagtg gcatcgtggt agggccaatc ctgtccgagt tcggaaggcc
     4441 actcgtggag ctgccgccat tgctgctcga ccggaacggc gagcgcccgc tcgcggtgca
     4501 gctcgccgac gcgctgcgtg ccgtcaccgc ggggggcacc ctgcgcgccg gggagcggct
     4561 gccgtccacg cgcgagctgg ctgccaccct cggcgtgagc cgcacggtca ccgcggccgc
     4621 ttacgaccag ctgctcgccg agggctgggc ggtgggacgg cgcggagcgg gcacgttcgt
     4681 cgtcggggct cctgccggac cacccgccgc ccgctggccc tcggcgccac cggaagccga
     4741 ccgggacggc gagcacgggg cgatctccct acgggccggc accccgtgcc tggaggtgct
     4801 cgaccgcgct gcctggcgcc gcgcctggcg cgcggcagcc gacccaccgc cggacgcggc
     4861 accggatccg gtcgggctgc cggcgttccg ggccgcagtg gtcgagcacc tgctgcgcca
     4921 ccgtgggctg ccggtcgacc aggcccaggt gctcgccacc gggggcagca ccgccgccgt
     4981 cgccgagctg gcacgaaccc tgcccgccgg agcgcgggtg gccgtcgagg agccggggta
     5041 ccagcggggt gtcgccgcgc tgcagaccgc cgggctcacc gtcgcccggg tgccggtgga
     5101 cggggcaggg ctcgtggtcg acgccctccc gcccgggctc gcggccgttt actgcacgcc
     5161 ggcacaccag tacccgctcg gggcgcggct gtccgcagca cgccggatgg cactggtgga
     5221 gcgagcgcgc gccgagggct tcctcgtgat cgaggacgac tacgacggtg agctgcgcta
     5281 cgacgtggct ccgctgccac tgctcgccgc gctcggaccg gacgtggtgg cgcacctcgg
     5341 gacagtcagc aagctgctca ccccgactct cggtgcgggc tggctggtgg tgccccccgc
     5401 tgtgcgcgcc gccatcctcg accagcggta ccgcaccggt gcccggcccg ctcccgccgg
     5461 gcagcgggtg ttcgtggcgc tggccgcgca cggcgacctg gcccggcatc tgcggcggct
     5521 gcgccgggag ctggccgagc gacgcgtcgc cgtcgccgtg gcggtacggg cggccggtca
     5581 catcctcgag ggcgacgcgg caggtgccca cgtcctcgtc ccactcccgg gcctaggcgc
     5641 ggagcagtcc gtcgtcgccg cagccgcggc cggcggcgtc accctcgacg ggctggcgcg
     5701 ccaccacgcg ggcgatccgg accgggcggg catcgtcctc ggttacgcgg agccgcggcg
     5761 ggccgagctc gagcgcggtc tcgcggtggt cacggacgtg ctggcgcgtt gtacgcccgc
     5821 ggttacggcg acagcgtcgc ccgtcgatct gccgggtagg ccgtcgcgcc gggatcgctg
     5881 aacagggacg ggacgactgc gggctgggct gcaggactgc ccaggcgcag gtcgacccgc
     5941 gcctgctcac ccacgggcag ctgcaccggg aacgtggcct gggcggtggc caccacggtg
     6001 tagtggcccg gctccaggcc ggtgattgcg tacgttccgt ccgccccggt cgtggtgacg
     6061 gccagggcgt ctccgtcggc gttgatgacg gccaccgtcg cggacggcag cggcgcaccg
     6121 tcggtgccgg tcaccctgcc ggtgaggccg gcgaccgtcg actgcagcag tacttcgacg
     6181 acggcgggcg cgtccgggac gacccggacc gcggccgcac cgggcacatg ccccggaagg
     6241 ctcgccgtca ccgtggtccg gcccgcgggg accccgggca gcacgaacgc gccgtcgggg
     6301 ccggtgcggg tgctaccgac cacccggccc gccgagtccg tcgcgagcac catggcacgg
     6361 gcgagcggcg ccccgctact ggccgcgcgg acggctccag tcaccgtgcc gtcaccgctg
     6421 agcgcaaagt ggtgcaccac gcccgagccg ttgaggacga tcgccgtcac gtccggccgg
     6481 aagccgggtg acgtggcgat ggccatgtac gttcccggtg gcatcccgcg cagggcgtaa
     6541 cggccgtcct ggtccacgcc cgagcgcgcg acctgccgtc cgtaagcgtc cgtcacggtc
     6601 acgaccccgt cggggaccgc gctgccgccc gatgtacgga cctggccgat gatctcgcct
     6661 gcggacgcgg tcaggactcg ggagaccggc ccgctcgggc cgggttccgg ctcgcgctca
     6721 gccgcaatgc tcgcgacgaa ctcggtcggg ccgaaccccg actcgccccc gaccgcggcg
     6781 agctcggcgc ccaccgacgc cgccggatcc gtcgagcggc gccctccgat gaaccaggag
     6841 gcgaccgcgg caaggacggt ggcacagatg gcgaaccaga aggccaccgt gaggccgttg
     6901 gcgaacggcc cggagatgag ctcagggaag aagctccgcc cggtcaggaa ggacgcctgg
     6961 tcgggcggca acgtgtccag caccccgccg aggagctgct gcacggggtt gtagccgagg
     7021 aaggcggcga acagcacggc cacgggcggc agcgcggcga tgcggctcgc gtcggccgct
     7081 ggaacgccgt gttccagcag cccctgctgc aggacagtcg ggaggtgctc ggacagcccc
     7141 gcgatcatga ggccgaagaa caggccgatg gagagcacca ttgccgagtt ctggaaggtc
     7201 gcggtcatcc cggcaccggc gccacgcgct ccagcgggca ggctgttcat cacgtcggcc
     7261 cggttgggcg acgtgaacag acccatgccc accccgttga ccagcaggac ggcggcgaag
     7321 ccgaggtagg ggaagtctat cggcaggatc aacagcagca cgaagctcag ggcagagacg
     7381 agcatgccgg cggtggccag cccgcgcccg cctatgcggt cggacagcac gcccgacacc
     7441 ggagccgcga tgaggaagcc gaccgtcagg ggcagcatgt agatgcctgc ccacagtggt
     7501 gtctgctcga agctgtagcc gtgcaggggc aaccagatgc cctgcagcca gatgatgagg
     7561 acgaactgca ggccaccccg gcccagcgac gccaagaggt tcgcgctgtt gccgagggtg
     7621 aagcggcgga tcttgaacag tgacagcgtg aagagcgggc tcgccacctt ggtctcgatg
     7681 atcccgaaca gcaccagcac gacgatcccg gcgatgatgc aggtgagcac gaacgggctc
     7741 gtccagccca tgacgtggtc gccgtagggc tggatgccgt aggtgatgcc caccagcacc
     7801 gcgaccaggc cgaccgcgaa cgtgatgttg ccccaccagt ccatcttcgc cggcgtgcgg
     7861 acgctggtgt cgtgcagttt gaagtaggcc cagaacgtcc cgaagacccc gaacggcacg
     7921 gacacgagga acacgaggtg ccagttcacc ggcgcgagca cgccgccgag gacgaggccg
     7981 atgaacgagc ccgcgatcgc cgcgacgccg ttgatgccga gcgcgaggcc gcgctggtgt
     8041 acggggaagg cgtcggtgag gatcgcgctg gagttcgcga acaggaacgc gccgccgatg
     8101 ccctggatga tgcgccaccc gatgagccac agcgcccccg cgctgccgct ctgccacgtg
     8161 atggcgagaa acacggacgc aacggtgaag atcgcgaacc cgatgttgta catccgcacg
     8221 cggccgtaca tgtcgccgag gcggccgaac ccgacgacca gcaccgccgt gacgaccagg
     8281 aagcccatga tcatccacag caggtagctg gtgttgcccg acgccagcgg gtcgaggccg
     8341 atgccgcgga agatgtcggg cagcgcgatc agcacgatcg aggagttgat cgtcgccatg
     8401 agcaacccga gggtcgtgtt ggacatggcg atccacttgt agccgggtcc cggctctgcg
     8461 cgcgtcggcc ctcccgccgc cggtccgcgc tcctgaactg ccgccacgtc caccactccc
     8521 gccgaggaga aaaaagcaat tagttgtccc atgctaaaca catggggcgg cggggatgtt
     8581 ccggcccggg taacgcctgc gtcaagcctg gtgcgggtag cccgtcgtgg tcgacggcac
     8641 gaacgtctcc ttgatcgtgc gaggactcac ccagcgcagc aggttgtaga tcgaacccgc
     8701 cttgtcgttg gtgcccgacg cgcggccgcc gccgaagggc tgctggccga cgacggcgcc
     8761 ggtcggcttg tcgttgacgt agaagttgcc cgcggcgaac cggagcgcct ccgtcgcgcg
     8821 cgcgaccgcg gcacggtccc tggcaatcac cgcaccggtc aggccgtacg gcgccccgcg
     8881 gtcgacgtgc tccaggacct gctcgaagtc gccgtcgtcg tacacgtgca cggcgagcag
     8941 cggtccgaag tactcggtgc ggaacacgtc gtggttgtgg tccgagccga cgatcaccgt
     9001 cggccggacg aagtagccgg tggagtcgtc ggcggtgcca ccggcgatga cctccagcgc
     9061 gggatccgag cgcgccgcct gttgcgccgc ggtcaccttg tcgaacgccc ggcggtcgat
     9121 cacggcgccg agaaagttgc ggaagtcggt gacgtcgccc atcgggagcg cctccacctc
     9181 gtcgacgagg tcgtcgcgca gccgggtcca gaccgaccgc gggacgaacg cgcgggaggc
     9241 ggccgagcac ttctggccct ggtactcgaa cgccccgcgg atgagggcgg tgcgcagcac
     9301 gtcgacgtcg gccgaggggt gggcgacgac gaagtccttg ccgccggtct cgccgaccag
     9361 gcgggggtag gtggcgtagg agccgatgtt ccgcccgacc tgccgccaca ggtgctggaa
     9421 c
//
LOCUS       DASRSC010000198        28715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_236606,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000198 DASRSC010000000
VERSION     DASRSC010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 28715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28715
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236606"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFE39_09735"
     CDS             <1..669
                     /locus_tag="VFE39_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005462938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HET6258918.1"
                     /translation="PTLLGHLESVPVAGPEVAAPFRMPVQYVIRPRTDQLHDYRGYAG
                     QVAAGTVRPGDEVVVLPGGARTTVACVDTADGPLHAAGAGRSITVLLADDIDIARGDV
                     IASAAAPPRLTSELDATLCWLADKPLRPGARLLLKHGARTTPVIVGALGDRLDTETIS
                     YVDAAEQLNINDIARVSLHTADQLPVDDYADIRATGSFLLIDPPTGNTLAAGLVGSPL
                     ALAP"
     gene            672..1517
                     /locus_tag="VFE39_09740"
     CDS             672..1517
                     /locus_tag="VFE39_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HET6258919.1"
                     /translation="MVPLQPAGSLLSRPPARRPGPGRTGARVDAAAPVLVAVAHGSRD
                     PRSAATVAALADEVRRQHPELDVRVSFLDLSAPRLPDVLAAVAAEGHRRAVVVPLLLG
                     HAFHARVDVPGEVSRAVRRHPQLDIATADVLGGSSLLEAAALERLAAVAGPLDDPGLG
                     VVLAAIGSSHAPANAAVAQVVSGWSRRFGWAGGTAAFATTTEPTVPQAIAALREQGAQ
                     RVAVAQWILAPGLLPDAIARAARAAGPDVLLAGPLAAHPAVAALIAARYTAAPGTAAT
                     SRAAG"
     gene            1692..1835
                     /locus_tag="VFE39_09745"
     CDS             1692..1835
                     /locus_tag="VFE39_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258920.1"
                     /translation="MTSTQLCPPGPAASPRGDLASMSGFLVTDLERFWPSRRGHAFDE
                     FCR"
     gene            1955..3046
                     /locus_tag="VFE39_09750"
     CDS             1955..3046
                     /locus_tag="VFE39_09750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6258921.1"
                     /translation="MPRLRAPALIPVVIAVIALLAATACSRAQPSGSETAAPVAGGTA
                     PEVRLGYFPNVTHAPAIIGVEQGLFTAELGSTKLTPQTFNAGGDAVNALLGGSLDVTY
                     IGSGPAINAFAKSDGAVRVIAGATEGGAQLVVKPDITDPQQLKGKTIATPQLGNTQDI
                     ALKVWLKKNGLTAGDGPEDVKIANLDNPRTLDAFRQGSVDGGWLPEPWSSRLVLDAGA
                     KVLLDERSLWPEGHFPTTVLLVRAEFLQKYPETVRALLRAHLKAIDLAQSNPAQAKTI
                     VNQGLQKLTGSALAGPVIDRAFQNITLDPDPLASTFPHLAQDSATAGITQKPTDLAGF
                     LDVAPLNAELQAVGKPPVDAAGLDKPGGS"
     gene            3046..3861
                     /locus_tag="VFE39_09755"
     CDS             3046..3861
                     /locus_tag="VFE39_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6258922.1"
                     /translation="MTATLDRHLGTQHRGAVDLQGVGKVFGRGADAVTALTDVDLRVQ
                     PGEFVVLLGASGCGKTTLLNLVAGLDRPTSGSVTVDSGRPAVMFQEPALLPWLDAGRN
                     VELPLRLSGVGRAARRERAAQLLDLVRLSGQARKRPHELSGGMRQRVALARALAATTT
                     NGSTSSPANGTSTDGGLLLMDEPFAALDAITRDFLQGELQRIWQATRAAVLFVTHDVR
                     EAVRLGQRVVLLSSRPGTVVREWDLGAAGVDREELTEEITGRLREVISTHART"
     gene            3848..4738
                     /locus_tag="VFE39_09760"
     CDS             3848..4738
                     /locus_tag="VFE39_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6258923.1"
                     /translation="MPAPEVVSETERTDTAAVGAGLDALDSPTEARKPVLRRVLATGL
                     PPLIALVLFLGAWQAVWASAMYPEYQLPAPLTVWQEFAGTVADGRIWQILWISLHRAV
                     LGFLASVVIATPLGLLIAKVPVVRAAIGPLLAGMQSLPSVAWVPAAVLWFGLTDTTIY
                     FVVLLGAIPSIANGLVAGIDQVPPILPRVGQVMGAGRIDSARLILLPAALPGYIAGCK
                     QGWAFSWRSLMAAEIIATSPQLGIGLGAYLKEGSDFNSMPGVIAAILLILLVGVGIEL
                     LAFRPLERRVLRARGLAAAT"
     gene            4882..5034
                     /locus_tag="VFE39_09765"
     CDS             4882..5034
                     /locus_tag="VFE39_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258924.1"
                     /translation="MSTTQPRDDPTVGGARRTALNEDWAATIVGLLLLALVLVLAGVV
                     PGWLVP"
     gene            complement(5219..7144)
                     /locus_tag="VFE39_09770"
     CDS             complement(5219..7144)
                     /locus_tag="VFE39_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HET6258925.1"
                     /translation="MTSAIAIVGAGPRGVGILERIGASVPELLPGTRHGTGLDVHLID
                     PYPAGAGRIWRYEQSPLLAMNSMAADVTMFTDDTVVCEGPIRSGPSFWDWAQELRAHG
                     DPDRLGPELAAELRAVTAATFPSRRLQSAYLTWVLHEVIAGLPAGVRVQIHHTRATGL
                     VEDATTQVVHLEDGQVLRVDAVVIASGHLDATPTDEERALAARAAGLGLRYLPPEQTT
                     DSDLSVIEPGETVIVRGMGLAFIDLVVLLFEGRGGRFEPAGPDDDALRYVPSGAEPHL
                     VVGSPRGAPYHSKTHYPLRAGRPPLPRFFGPAAVDPLIASAEPVHLRAQVWPLMAKEI
                     AWGWYHELFLGHPQRVRLTWDDFASRYAELDWGTPAMDELLTAAVPDAVDRIDFAALD
                     RPLDGLRAPDLAALQPLVRERIAEDLRQHLDPAHTPHLGAFVAMLSVYGEITRLRGSG
                     VLSARSRAFDMSWWQGFFNSVASGPPGFRVRELLALSQAGFVEFLGAGMWVDVEDGAF
                     SAGSATLQGAPPVTASVLVDARLPEPSAARTVDPLLAGLMRDGDACEDVLVDDDGTVL
                     RNTGLVRVRPADGALIDASGSAHPRRFAVGPHTTVKVAGAFTRPGMNSQSLRYNDALA
                     RAVLRSLPASTAAASAA"
     gene            complement(7576..8376)
                     /locus_tag="VFE39_09775"
     CDS             complement(7576..8376)
                     /locus_tag="VFE39_09775"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HET6258926.1"
                     /translation="MPGILVPGHDLSPVLLHVPHAGTEIPGWTRPHLLLDDRELAAEV
                     AALTDHHTDRIAAAAAERARVRPWALINPVSRFVVDPERFPDEREEMARVGMAAVYTR
                     GTRRQRLRVEDGAHREALLAAYYAPWALAVQEAVDGRLAAAGRAVLLDVHSYPSAPLP
                     YELHASGPRPAVCLGTDAVHTPEWLFAAARVAFAPVGTVGLNSPFAGTYVPLAHYGTD
                     RRVLSIMIEIRRDGYLREPSGAPTAGAGRVAAALADLVDAVTPAPNPY"
     gene            8602..9717
                     /locus_tag="VFE39_09780"
     CDS             8602..9717
                     /locus_tag="VFE39_09780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6258927.1"
                     /translation="MPVEFLGIGSTNNGSETDPRTGPAFDKEYTLALARAHEETGWDR
                     VLTAYGSGSPDPAQAAALIATHTERLQLLLAHRPNVSYPTFAAKTVATLDQISDGRLT
                     LHVITGGNDHEQQREGDTLPKDRRYDRTREAIQIFKKAWTSRTPFDFHGEHYDFTDFV
                     LDVEPAQKPRPRISFGGSSPAAYKVGAEEADIYALWGEPLAGTAEQIATITELAGAAG
                     RPAPTFQVAFRPILGRTEEEAWEKAYATVARIQDRTKGGTSQLTRRHKLTDPENAGSQ
                     RLLEVAAQGERFDRALWTVTAKATGGAGNSTALVGTPETVAAALLDYYDLGVRILSAR
                     GYDLLEDAKEFGREVIPLVRAEVARRDAEQARTAVPA"
     gene            complement(9791..10069)
                     /locus_tag="VFE39_09785"
     CDS             complement(9791..10069)
                     /locus_tag="VFE39_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258928.1"
                     /translation="MKWLFPPRRFPAVIMFEDWLVGQTSAPRPRSTLAQLMLDRRFAE
                     VDLGSQRNRDAVRLLLDDFERDAEGQWVRSDPLPSRAGAEPSRPPSHR"
     gene            complement(10135..10500)
                     /locus_tag="VFE39_09790"
     CDS             complement(10135..10500)
                     /locus_tag="VFE39_09790"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET6258929.1"
                     /translation="MPFTALLGATALRAGPDEVALRLDWDATRCTSGGLLHGGALMAL
                     ADAAGGWCAFLNLPDGATGTATVSSATNFLRAVTSGHANAVARVLHKGRRTIVVDTEV
                     HDAAGKLAARVTQTQAVLR"
     gene            10624..11421
                     /locus_tag="VFE39_09795"
     CDS             10624..11421
                     /locus_tag="VFE39_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6258930.1"
                     /translation="MTAATATGTAFGSLLRDWRQRRRLTQLDLGLQADVSARHLSFLE
                     TGRSKPSREMVLHLSEELDVPLRGRNELMMAAGFVPAYTEHGLDDAELGEVQRSLRRI
                     LDSQEPYPAVLVDGSWNLLAANRAVALLTDLVDPALLAAPANVLRVSLHPRGLAPYVV
                     DLEEYAAHLVSRVRRQAERAATTGLRALLGELVGYCRDAGLDPTAQPHDRIVLPLRLR
                     HPDQELALFSTVAVLGAPLDVTLEEVAIESFFPADDATAAYLRERFA"
     gene            11543..12142
                     /locus_tag="VFE39_09800"
     CDS             11543..12142
                     /locus_tag="VFE39_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258931.1"
                     /translation="MGAGLRVGAAAARGLLDAAVAGVSGALVPRVEQRAQDMLHASVI
                     EVELTAAARLSGTDTVVADLPAGWATVLSRMFQTGRDLSGRAVAAAVGGTRAVPGRAC
                     GGGRRADRRPRRPCRARRLRHAARARGPGRTASRCSSPTGWRTSRHADQILVLEWGRL
                     IEHGRHEQLIALGGTYHELFTLQARAYGESAGEPDTVPA"
     gene            12139..12909
                     /locus_tag="VFE39_09805"
     CDS             12139..12909
                     /locus_tag="VFE39_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6258932.1"
                     /translation="MTDELVHLDVAAGVATITLDSPANRNALSAQLRRELLAHLDSAI
                     ADDTARVIVLSHTGRVFCAGMDLKESRGAGAGDQGVNEFPAILERIWAAPTPVVARLA
                     GPARAGGVGLVAACDIAVAAEDATFAFSEVRIGVVPAIISVTVLPRLLPRAAHELFLT
                     GETFDAARAAQIGLVNSAVPGVHLDAEVHRYTEALRLGAPGALAATKEMLRRERPSAM
                     AEQFAEMQELSARFFAGEEGQEGMRAFIEKRPPSWAVV"
     gene            complement(13031..13630)
                     /locus_tag="VFE39_09810"
     CDS             complement(13031..13630)
                     /locus_tag="VFE39_09810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HET6258933.1"
                     /translation="MDNADHMSQSIDLAVAAISGAEQARYGDSSPCSDFAVRDVVNHI
                     AFGLLLAHRCATREAWDPSWTAADRTPFLSGRPESEWAAACGEQGRATAQAWASPAAW
                     EGESHMAGTAMPAAVVGSIMTAEFVVHAWDLAVATGQPLDVPSGLGEVVLEGVEAMAP
                     SGRDGGWFGAEVQVPADAPPFERALAASGRDPRWSPTDR"
     gene            complement(13685..14089)
                     /locus_tag="VFE39_09815"
     CDS             complement(13685..14089)
                     /locus_tag="VFE39_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HET6258934.1"
                     /translation="MDVRETNRRVIEQFRGGGPVEGMHRDRLVLLTTAGRRTGRPHTT
                     PMMFHRDDDVLLVVASNIGAPQHPDWYLNLVVDPAVDIEIDGERYPGAAEALEGDRRE
                     ETWAMLTDTYPFFADHETRTERTIPVVALRRS"
     gene            14175..15023
                     /locus_tag="VFE39_09820"
     CDS             14175..15023
                     /locus_tag="VFE39_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HET6258935.1"
                     /translation="MDVLETALDEVLDRALGVLSRRPLVALTGAGLSTDSGIPDYRGP
                     GSPRRTPMTYQDFLSGEPAQRRYWARSYVGWARMAHAEPNAGHRALAELERVGALHGL
                     ITQNVDGLHSAAGSRALIDLHGRIADVVCLGCGRRSRRDELQRRLDALNPGFVDAAGA
                     AVEAAPDGDAELAATDGFRLARCTGCGGVVKPDVVFFGENVPRGRVAGAYAMVDAAGA
                     LLVAGSSLTVMSGLRFVRHAHKRGTPVVIVNRGATRGDELAEVLVDAGCSEVLPMIAA
                     IAAAAG"
     gene            complement(15035..15226)
                     /locus_tag="VFE39_09825"
     CDS             complement(15035..15226)
                     /locus_tag="VFE39_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258936.1"
                     /translation="MMRAVADWWDAVELWITQLPFPMQVVLAVLVMLPLCWGTAAGVD
                     RVVDLVVDRVSVRRGTGRD"
     gene            15304..16269
                     /locus_tag="VFE39_09830"
     CDS             15304..16269
                     /locus_tag="VFE39_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6258937.1"
                     /translation="MFAVYAAEPNADAPLDSLTVGERPEPEVPDGWVAVTVSAASLNM
                     HDLWTLRGVGIKPEQFPMILGCDGAGTLEDGTEVVLHSVIGDATLHGDETLDPKRTLL
                     TEKHQGTFAETVVVPKRNAVPKPAGLSAVQASVMGTAWLTAYRMLFVKSGLRPGQTML
                     VQGASGGVSTALVQLGSAAGMRVWVTGRTEDKRAVAEKLGAHATFPSGERLPERVDAV
                     FETVGDATWSHSMRSLRPGGAIVVCGSTSGPNPGADLQRLFFLQLRVIGSTMGTRDEL
                     SDLLRFVAEAGIKPEIGLELPMDQAEGGFRAMLEGETSGKIVFTR"
     gene            16418..16840
                     /locus_tag="VFE39_09835"
     CDS             16418..16840
                     /locus_tag="VFE39_09835"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6258938.1"
                     /translation="MTGLVELLDRLDRAVAAALSPVTSAEGVSRDGWRVLLMLARGGG
                     RSMGEIASHTALPAPTATRVVDRLVAGRLAYRRTDPEDRRRVLVHLAGDGRAVVERVC
                     GRVERRAGEALGSPHTPERAQLAELLEALACQDLTALG"
     gene            complement(16918..17118)
                     /locus_tag="VFE39_09840"
     CDS             complement(16918..17118)
                     /locus_tag="VFE39_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258939.1"
                     /translation="MQRPAAVRAGDAVRVLDRPAHGVTVAGLMAALTGARDEIPAVVA
                     AREHLGARGRDWLDRALGAMRN"
     gene            complement(17560..17982)
                     /locus_tag="VFE39_09845"
     CDS             complement(17560..17982)
                     /locus_tag="VFE39_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258940.1"
                     /translation="MTNLPVVPGGGSPGVAALVLLVVLLVGVGAGRTKRRWTLLLAGL
                     LGVLVLGIVIVALREPAGTDDRASAITALYTVAVPLAVAFTAGWLCGRGTWFRRLVVL
                     GIAVLLLAAFPYAAAGRVTADTLFRAPAAAPQGEGTSG"
     gene            complement(18063..19910)
                     /gene="lepA"
                     /locus_tag="VFE39_09850"
     CDS             complement(18063..19910)
                     /gene="lepA"
                     /locus_tag="VFE39_09850"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HET6258941.1"
                     /translation="MSSFADKTFTPPELIRNFCIIAHIDHGKSTLADRMLQLTGVVEG
                     RAMRAQYLDRMDIERERGITIKAQNVRLPWQVGETEHVLHLIDTPGHVDFTYEVSRAL
                     EACEGAILLVDAAQGIEAQTLANLYLALEKDLTIIPVLNKIDLPAADPDRYAAEIAHI
                     VGCDPDDVLRVSAKTGAGVRELLDEVVRQVPAPVGDPDAPARAMIFDSVYDTYRGVVT
                     YIRVVDGKITPRERIKMMSTGTTHELLEVGIVSPEPKPSIGLGVGEVGYLITGVKDVR
                     QSKVGDTITSQRHGATEPLTGYREPRPMVYSGLYPVDGSEYPDLREALDKLQLNDAAL
                     TYEPETSAALGFGFRCGFLGLLHLEITRDRLEREAGLDLISTAPNVVYRVIKDDGTEM
                     TVTNPSDWPTGKVAQIFEPVVKVTILAPSEFIGTIMELCQGRRGQLGGMDYLSESRVE
                     LRYTMPLAEIIFDFFDALKSRTRGYASLDYEEAGEQEAELVKVDIMLQGEPVDAFSAI
                     RHKDDAYSYGTSMTTKLRELIPRQQFEVPIQAAIGSRIIARENIRAIRKDVLAKCYGG
                     DITRKRKLLEKQKEGKKRMKTIGRVEVPQEAFVAALSTDSAGDAKAGKK"
     gene            complement(20615..21322)
                     /locus_tag="VFE39_09855"
     CDS             complement(20615..21322)
                     /locus_tag="VFE39_09855"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET6258942.1"
                     /translation="MGTALVLVHDAVPGRGEQEVGTVGPALTGLGLDPLVTTLVGGGP
                     GVPDLAEVAVLVVLGASESAADDTVPWLAAELDLMGQAVATGTPVLGICFGAQALARV
                     LGGAVGRAPRSERGFVPLGTAAPDLLPAGEWLQFHDDAFTLPPGAQELASNEVGLQAF
                     AHGPHLGVQFHPEITPAAFAAWVGSWEESGVAEVLAAEVDLPALRADLVARSAATEDA
                     CHDLVARFATHAGVLVP"
     gene            21435..21899
                     /gene="cynS"
                     /locus_tag="VFE39_09860"
     CDS             21435..21899
                     /gene="cynS"
                     /locus_tag="VFE39_09860"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: protein motif:HMM:NF002773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HET6258943.1"
                     /translation="MTTTPQPPLDRKQAATAVLAAKRRTGVRWADLGKTIDRSTEWTT
                     AALLGQHPLTPEQTAALGAVLELDDPVIDALGLPPVRGRDVVDTSEPIVYRLQEMVQV
                     YGSTITELVREEFGDGIVSAIDFEMDLVRVPDPKGDRVRITLNGKFLPYRTW"
     gene            22055..23860
                     /locus_tag="VFE39_09865"
     CDS             22055..23860
                     /locus_tag="VFE39_09865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6258944.1"
                     /translation="MTLCSSVAINRPSECSLVTGGPSCRCPEDAPRLADGQIVVRATD
                     KFRWQQQASPDGRSAARRGLDGLLAHARRNSPSVVVSELLRMSILVRILRPNRPGDEI
                     KAEVEALLQEYMELAELDDDPRRLGEAATLRAHRTAMFGKAENALADTAAAHAILTDL
                     TSPGPGEDPAEWSRLLARSLNVLVLVLLKLGSHELADEVSQRAIAMSENCASTMDRLV
                     YQLNRVRLQLSWAFRLERGGREAAAATRFVGAAQTAHTAALLWGPAHDRPAGGPPAVE
                     ECSIIGSAYALNQPGPEHLEMLARLTPMAFFPDDLKLIAIATARCLLAAGRPGDAAAS
                     LAPLHRELQADTSGGLVLAVHREFAMIDDLARGAPRRSDALASYTAALEDELWALREA
                     RLTALRSHSEHHRLTREHGAVAAQALQDPLTGLPNRRALDLRLLEVTTSSACQPCAVA
                     LIDLDGFKDVNDARSHAAGDTVLREVAACLRTILRSQDLVARYGGDEFVVVMPATPLA
                     EARAALQRAADAVAALPADVAAGVTMSVGVVRAPLDSEPAAALAAADAAMYRAKHSGG
                     NTVISGPGADTSAGALVSTHHVVGRQGVTTSPRTA"
     gene            complement(23835..24587)
                     /locus_tag="VFE39_09870"
     CDS             complement(23835..24587)
                     /locus_tag="VFE39_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6258945.1"
                     /translation="MTVVLVHGNPETSAVWDLLSERLGALGYDDQVRLSPPGFGAPVP
                     EGWTATVDEYRDWLVDELEEIGRPVDLVGHDWGGGHVLNVAMTRPDLLRTWCSDILGV
                     YDPEYVWHELAQGWQTPGVGETAIAQRLAIPVEQAAAALADRGMTPAVAARVAAGFDA
                     VMGECILALYRSAAQPVMAELGTGLEAAAARPGLALLPTEDHFVGTDEQRRRAAARAG
                     ARLEVLDGLGHWWMTQDPDRGAAALTRFWDSL"
     gene            24749..25009
                     /gene="rpsT"
                     /locus_tag="VFE39_09875"
     CDS             24749..25009
                     /gene="rpsT"
                     /locus_tag="VFE39_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET6258946.1"
                     /translation="MANIKSQIKRVKTNEKRRQRNKSVKSAVRTAVRRFREAAEAGDT
                     EKATELQRAASRALDKAASKGVIHKNQAANRKSSLSKRVGAL"
     gene            complement(25091..26065)
                     /locus_tag="VFE39_09880"
     CDS             complement(25091..26065)
                     /locus_tag="VFE39_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET6258947.1"
                     /translation="MSASPPPAPLRLVLGEEELLVERAVEQIVAEVRAVEPGAELRRY
                     RAAELTPVDLADNLSPSLFSEGRVIVLQAAHEVGKELAAAIVEQSADPAEGIVLVVLH
                     AGGARNKALADALRKGGAVVTTCNRITRFEERSDFVRAEVRRAGGRIAPDAVGVLIEA
                     VGSDLRELAAATNQLVADTGGTVDERAVRRYHRGRAEASGFAVADKAMAGDRPGALEA
                     LRWALVLGVAPVLVADALADGLRTLARVGAAGRGDPNRLGGTLGMPPWKVRKAQAQVR
                     AWRPESLAVAFDAAAQVNADVKGAAADPGYALERAVLRICAARESRAV"
     regulatory      26202..26320
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            26329..27600
                     /gene="thrC"
                     /locus_tag="VFE39_09885"
     CDS             26329..27600
                     /gene="thrC"
                     /locus_tag="VFE39_09885"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET6258948.1"
                     /translation="MTLTADVSASTSYDLGPARALSCRECSHQIPLAAEFACPQCFGP
                     LEVAYDFGTVTRESIEAGPKSIWRYQQLLPVPSTVREHANTEPGLTRLIKADRLGVAL
                     GLRNLWVKDDTGNPTHSFKDRVVAVALAAARELGFSVLACPSTGNLANAVAAAAARAG
                     WDSIVLIPSSLEQAKVLTTAVYDGTLLAVDGNYDDVNRLATELAAEHEDWAFVNVNVR
                     PYYAEGSKTLGYEVAEQLGWRLPEQIVVPVASGSQLTKVDKGFTELGSLGLVEPTPYR
                     VFGAQATGCSPVAKAFEDGHDVVQPVRPDTIARSLAIGNPADGPYVLDTVRRTGGAMA
                     HVSDAEVVDGIRLLARTEGVFAETAGGVTVATAKKLVETGKLDPDAETVLLITGDGLK
                     TLDAVAGQIGPSATVPSTSKAVREALAARGL"
     gene            complement(27707..28354)
                     /locus_tag="VFE39_09890"
     CDS             complement(27707..28354)
                     /locus_tag="VFE39_09890"
                     /inference="COORDINATES: protein motif:HMM:NF037041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258949.1"
                     /translation="MSLIHRAQLTPTKRELIAEWLPSRPWSRNAPAGQAELIGAYRFD
                     DPSGAVGIETHLVRTGDAQVLQVPLTYRGAPLAGAEDFLVGTTEHSVLGRRWIYDGCG
                     DPVYGAVLATAILAGGREAEEWVEQADGRSERREPAVRVVGSGTPGVEAPSVDSVSAV
                     DDATTTTVGASGLELVVRRVLGGLTHVDGDVETLTGTWPGSAEPTSLAYARRRPS"
     gene            complement(28373..>28715)
                     /locus_tag="VFE39_09895"
     CDS             complement(28373..>28715)
                     /locus_tag="VFE39_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258950.1"
                     /translation="QAGALMTELAEAPRPGSLDALTAASHHRPLATDLRTLPAADLCT
                     LRAVSQQRFGSFYSELDQLRAYDVIVRFPHVTPAETRPGRPGPRPPRGPGGVLPVEAG
                     VTSAPLAALAH"
BASE COUNT         4078 a        10691 c        10063 g         3883 t
ORIGIN      
        1 ccgacgctgc tcgggcatct ggagtcggtg cccgtcgccg gcccggaggt cgccgcgccc
       61 ttccgcatgc cggtgcagta cgtgatccgt ccccgcaccg accaattgca cgactaccgc
      121 ggctacgcgg gacaggtcgc cgccggcacg gtgcggccgg gcgacgaggt ggtcgtgctg
      181 cccggcggtg cccgcaccac ggttgcgtgc gtcgacacgg cggacgggcc gctccacgcc
      241 gccggcgccg ggcgcagcat cacggtcctg ctggccgacg acatcgacat cgcccggggt
      301 gacgtcatcg cctcggcagc ggcgccaccg cgcctcacga gcgagctgga cgccacgctg
      361 tgctggctcg ccgacaagcc gctgcggccg ggggcccggc tgctgctcaa gcacggcgcc
      421 cgcaccacgc cggtgatcgt cggtgcgctc ggcgaccgcc tcgacaccga gaccatctcc
      481 tacgtggacg cagccgagca gctgaacatc aacgacatcg cgcgcgtctc gctgcacacg
      541 gccgaccagc tcccggtgga cgactacgcc gacatccgcg ccaccggcag cttcctgctc
      601 atcgacccgc ccaccggcaa cacgctcgcg gcgggcctgg tcggcagccc gcttgcgctg
      661 gccccctagc catggtgccg ctccagcccg ccggctccct cctgtcgcgt ccccccgcgc
      721 gcaggccagg gccgggccgg accggcgccc gggtcgacgc ggcggcgccc gtgctggtcg
      781 ccgtcgcaca cggcagccgc gacccgcggt cggcggccac ggtcgcggcg ctcgccgacg
      841 aggtgcgccg gcagcacccc gagctcgacg tgcgtgtgag cttcctcgac ctgtccgcac
      901 cccggctacc ggacgtgctc gctgcggtgg ccgccgaggg gcaccggcgc gcggtggtcg
      961 tcccgctgct gctcgggcac gcctttcacg ccagggtcga cgtgcccggt gaggtgagcc
     1021 gcgcagtgcg gcggcatccg cagctcgaca tcgcgacggc cgacgtgctc ggtggcagct
     1081 ctctgctgga ggcggccgcg ctggagcgtc tcgccgccgt cgccggcccg ctcgacgacc
     1141 ccgggctcgg cgtcgtgctc gccgccatcg gttcctcgca cgcacccgcg aacgcggccg
     1201 tcgcgcaggt ggtcagtggc tggtcccggc gtttcggctg ggcgggtggc acggcggcgt
     1261 tcgccacgac gaccgagccg accgtgccgc aggcgatcgc cgcgctacgg gaacaggggg
     1321 cacagcgggt ggcggtggcg cagtggatcc tcgcccctgg cctgctcccg gacgcgatcg
     1381 cgcgggcggc gcgcgccgcg ggcccggacg tgctgctcgc cggcccactc gcggcccatc
     1441 cggcggtagc ggccctgata gccgccaggt acacggctgc ccccggcacc gccgccacct
     1501 cccgtgccgc aggctgactc cgacccgtgc gcgacatgga cacatctcgc tgttcggcag
     1561 ccgcctcggt ccgcgtcagg tggccatgtc gcgcctccgt ccgcctgttc cggtgtacgg
     1621 acatttcgtc gcgttcccgg cacgcggacg cctggttgtc cttgtccacc cgcgcgacgc
     1681 acgatggtgc cgtgacctcg acgcagctgt gcccgccggg ccccgccgcg tcgccgcgcg
     1741 gggacctcgc ctcgatgtcg ggcttcctgg tcacggatct cgagaggttc tggccgtccc
     1801 gccgcggcca cgccttcgac gagttctgtc gctgacctct cgcaccgccc gctgatccgc
     1861 tgccgcgctg tcgcgcgccc atcagcacgc gtgccgcagc ccgcccggcc atccgctccc
     1921 cgggcacgcc ccatccagaa caggacttca tccgatgccc cggctccgcg cccccgccct
     1981 gatccccgtc gtgatcgccg tgatcgccct gctggccgcc accgcgtgct cgagggccca
     2041 gccctcgggc tcagagacgg cagcgcccgt cgccgggggg acggcgccgg aggtgcggct
     2101 cggatacttc cccaacgtca cgcacgcacc ggcgatcatc ggcgtcgagc agggcctgtt
     2161 caccgccgaa ctgggcagca ccaaactgac cccgcagacc ttcaacgccg gcggtgacgc
     2221 ggtcaacgcg ctgctcgggg gatcgctcga cgtcacctac atcggctccg gcccggcgat
     2281 caacgccttc gcgaagtccg acggtgccgt ccgggtcatc gccggtgcca ccgagggcgg
     2341 cgcgcagctc gtcgtgaagc ccgacatcac cgatccgcag cagctcaagg gcaagacgat
     2401 tgccaccccg cagctgggca acacgcagga catcgcgctc aaggtgtggc tgaagaagaa
     2461 cgggctgacc gcgggtgacg gccccgagga cgtcaagatc gccaacctgg acaacccgcg
     2521 cacgctcgac gcgttccgcc agggcagcgt cgacggcggc tggctgcccg agccgtggag
     2581 ctcgcgactc gtcctcgacg ccggcgcgaa ggtgctcctt gacgagcgca gcctgtggcc
     2641 ggaggggcac ttcccgacca ccgtgcttct cgttcgcgcc gagttcctgc agaagtaccc
     2701 ggagacggtc cgcgcgctgc tgcgggcgca cctgaaggcg atcgacctgg cgcagagcaa
     2761 cccggcgcag gccaagacga tcgtcaacca gggcctgcag aagctcaccg gcagtgcgct
     2821 cgccgggccg gtgatcgacc gcgccttcca gaacatcacg ctcgaccccg atccgctcgc
     2881 ctcgacgttc ccgcatctcg cgcaggacag cgcaaccgcc gggatcaccc agaagccgac
     2941 ggacctggcg gggttcctcg acgtcgcgcc gctcaatgcc gaactgcagg ccgtcggaaa
     3001 gccccccgtg gacgccgcag gactcgacaa gcccggaggc agctgatgac cgccactctc
     3061 gaccgtcacc tcggtaccca gcaccgcggc gctgtcgacc tgcagggcgt cggaaaggtc
     3121 ttcggtcgcg gagccgatgc ggtcaccgcg ctgacggacg tcgacctccg ggtccagccg
     3181 ggtgagttcg tcgtgctgct gggcgcgtcg ggctgcggca agacgacgct gctcaacctc
     3241 gtcgccgggc tggaccggcc cacctccggc agcgtgacgg tcgacagcgg caggcccgcc
     3301 gtcatgttcc aggagcccgc cctgctcccg tggctcgacg caggccgcaa cgtcgagctc
     3361 ccgctgcggc tgtccggggt cggccgcgcc gcccgccggg agcgcgcggc gcagctgctg
     3421 gacctcgtcc gcctgagcgg gcaggcgcgc aagcggcccc acgagctgtc cggcggcatg
     3481 cgccagcgtg tcgcgctggc ccgcgcgctc gccgccacga cgacgaacgg gtcgacgagc
     3541 tcgcccgcga acgggacgag caccgacggc ggtctcctcc tgatggacga gccgttcgcc
     3601 gcgctggacg ccatcacccg cgacttcctg cagggcgagc tgcagcggat ctggcaggcc
     3661 acccgtgcgg ccgtgctgtt cgtgacccac gacgtccgcg aggccgtccg gctcgggcag
     3721 cgggtcgtgc tgctctcgtc gcggccgggc accgtcgtgc gcgaatggga cctcggcgcc
     3781 gcgggggtgg atcgcgagga gctcaccgag gagatcaccg ggaggctccg ggaggtgatc
     3841 tcgacgcatg cccgcacctg aggtcgtctc cgagaccgag cgcaccgaca cagccgcggt
     3901 cggcgccggg ctggacgcgc tcgactcgcc caccgaagcc cgcaagccgg tgctgcgccg
     3961 ggtgctggcc accgggttgc cgccgctgat cgcgctcgtc ctcttcctcg gcgcgtggca
     4021 ggccgtgtgg gcctcggcga tgtacccgga gtaccagctt cccgcgccgt tgacggtctg
     4081 gcaggagttc gccggcaccg tcgccgacgg ccggatctgg cagatcctgt ggatctcgct
     4141 ccaccgcgcg gtcctcgggt tcctcgcctc cgtggtgatc gccaccccgc tcgggctcct
     4201 catcgcgaag gtccccgtcg tgcgggccgc gatcggcccc ttgctggccg gcatgcagag
     4261 cctgccgtcg gtcgcctggg tgcccgcggc tgtgctgtgg ttcggcctga ccgacaccac
     4321 gatctacttc gtagtgctgc tcggtgccat accctcgata gccaacgggc tcgtcgccgg
     4381 catcgaccag gtaccaccga tcctgccgcg cgtcgggcag gtgatgggcg ccggacggat
     4441 cgactcggcg cgcctgatcc tgctgcccgc cgcgctgccg ggctacatcg caggctgcaa
     4501 gcaggggtgg gcgttctcct ggcgctccct gatggcggcg gagatcatcg cgacctcccc
     4561 ccaactgggc atcgggctgg gcgcgtacct caaggagggc agcgacttca acagcatgcc
     4621 cggggtcatc gccgccatcc tcctgatcct gctggtcggc gtcggcatcg agctactggc
     4681 gttccggccg ctggagcgcc gcgtcctgcg ggcccgcggc ctggccgcgg cgacctgatg
     4741 cccgcgctcg cgctgtcccg tcgccggcat gtcgactact gccgcgtcct cagcacgagc
     4801 tgtcccgcct gatcggctcc cgccccggtg gcgcgtgcac gtggtgcgcc ggggcctcga
     4861 tcggacgaca ggacgagaac catgagcaca acgcaacccc gcgacgaccc gacggtcggc
     4921 ggtgcccgcc gcaccgccct caacgaggac tgggccgcga cgatcgtcgg cctcctgctc
     4981 ctcgcgctcg tgctcgtgct cgcaggcgtg gtgcccggct ggctggtgcc ctgatgagca
     5041 gcgctcgcgg ccgccgcctg cgcaacaaca ccagcatgcg gcggctcgcc gcgctgccgt
     5101 ccgtccgctg gcagtgacgg tatgacggcg aggccgaggc gacgaagccc tgcttccccg
     5161 tccactcgcg gagccgaaga cgcgctcggg agccgggtcc cgccgagggc tggtacggct
     5221 atgcggcgga ggcggccgct gtgctcgcgg ggaggctgcg cagcaccgcg cgggccaggg
     5281 cgtcgttgta gcgcaggctc tgcgagttca tccccggtcg ggtgaaggcg cccgccacct
     5341 tcaccgtggt gtgcgggccc accgcgaagc ggcgcggatg ggccgacccg gaggcgtcga
     5401 tcagcgcgcc gtcggccggg cgcaccctga ccagcccggt gttgcgcagg acggtgccgt
     5461 cgtcgtcgac gagcacgtcc tcgcaggcgt ccccgtcgcg catcaggccc gcaagcagtg
     5521 gatccacggt gcgcgcggcg ctcggctccg gcagcctggc gtcgacgagc acgctcgcgg
     5581 tgacgggcgg tgcgccttgg agcgtcgcgc tgcccgcgct gaacgcgccg tcttcgacgt
     5641 ccacccacat cccggccccg aggaactcga cgaaccccgc ctgggacaac gcaagcagct
     5701 cacgcactcg gaacccgggc gggccgctgg cgacggagtt gaagaagccc tgccaccagc
     5761 tcatgtcgaa cgcgcgcgac cgagccgaca gcactccgga cccgcgcagc cgggtgatct
     5821 ccccgtatac cgagagcatc gcgacaaacg cacccagatg cggcgtgtgg gccgggtcga
     5881 ggtgctgacg cagatcctcg gcgatgcgct cacgcaccag tggctgcagc gcggcgaggt
     5941 caggcgcgcg caggccgtcg agcgggcggt cgagtgccgc gaagtcgatc cggtcgacgg
     6001 cgtcgggcac ggcggcggtg agcaactcgt ccatcgccgg cgtaccccag tccagctcgg
     6061 cgtaccggga ggcgaagtcg tcccaggtca gccgaacccg ctgcgggtgg ccgaggaaca
     6121 gctcgtggta ccaaccccag gcgatctcct tggccatcag tggccacacc tgtgcgcgca
     6181 ggtggacggg ctcggcggag gcgatcaacg gatcgaccgc ggcgggtccg aagaaccgcg
     6241 gcagcggcgg acgcccggcg cgcagggggt agtgggtctt ggagtggtag ggcgccccgc
     6301 gcggcgagcc gacgaccagg tgcggctcgg cgcctgatgg gacatagcgc agggcgtcgt
     6361 cgtccggccc cgcaggctcg aaccggccgc cgcgcccctc gaacagcaac accacgaggt
     6421 cgatgaacgc gagccccatc ccgcggacga tcaccgtctc ccccggctcg atcaccgaca
     6481 ggtcggagtc ggtggtctgc tcgggcggca ggtagcgcag ccccaacccg gccgcgcggg
     6541 cggccagggc gcgctcctcg tcggtggggg tggcatcgag atgcccggag gcgatcacga
     6601 ctgcgtcgac ccggagcacc tggccgtcct ccaggtgcac gacctgtgtc gtcgcgtcct
     6661 cgaccagccc agtggcgcgg gtgtggtgga tctgcacgcg cacgcccgcg ggcagtccgg
     6721 cgatcacctc gtgaagcacc caggtcaggt aggcgctctg caaccgcctg ctcggaaaag
     6781 tcgccgccgt gacggcgcgc agctcggctg ccagctcggg tcccagccga tcggggtcac
     6841 cgtgggcgcg cagctcctgc gcccagtccc agaacgaggg tcccgaccgg atcggtccct
     6901 cgcagacgac cgtgtcgtcg gtgaacatgg tgacgtcggc cgccatcgag ttcatcgcca
     6961 gcagcggaga ctgctcgtag cgccagatcc ggcctgcgcc ggcagggtag ggatcgatca
     7021 ggtgcacgtc gaggccggtt ccgtggcggg tgccgggaag cagctcgggc acgctcgcac
     7081 cgatgcgctc gagtatcccg acgccgcgcg gacccgcgcc gacaatcgcg atcgcggagg
     7141 tcacagaaaa cttcctctcg gcaggcggac gtggacggtc cgtgcctcag agggctgcgc
     7201 tggtgcggca caggtccacg tgccaccggc gcgccagctg tgtccccaca caccgactgt
     7261 agatcggcac agcgggcggg gccaccgaaa ccgacgagca tcacgccctg ccggcggcgg
     7321 aacgcgtgct gcggaaccac ggctgatcgc tgcgagatcc gggatccagc ccggcgaagg
     7381 gccgaaacct tgatctcgcg gcgatcaacg acccgcgcga ccggaaaggg cgactcgtgg
     7441 acgggaaacc ggcgactcgc gggggactcg cgggcgggac aagccggcgc ggacgggcga
     7501 ctcgggggcg ggaaacgggc gactcgcgcg accggaacgg gcgactcgcg ggcgggaaac
     7561 gggcgactcg cggggtcagt acgggttcgg ggcgggcgtg actgcgtcga cgaggtccgc
     7621 gagggcggcg gctacgcgcc cggcacctgc ggtcggggcg ccggagggct ccctcaggta
     7681 cccgtcgcgg cggatctcga tcatgatcga aagcactctc ctatcagtgc cgtaatgggc
     7741 gagcgggacg taggtaccgg cgaacgggga gttcagcccg acggttccga ccggggcgaa
     7801 ggccacccgc gccgccgcga agagccattc cggcgtgtgt acggcgtcgg tgcccaggca
     7861 caccgcgggt cgggggccgc ttgcgtgcag ctcgtacggc aggggtgccg acgggtagga
     7921 gtgcacgtcc agcagcacgg cgcggccggc ggcggcgagc cggccgtcga ccgcctcctg
     7981 caccgccagg gcccacggcg cgtagtacgc ggcgagcagg gcctcgcgat gcgcgccgtc
     8041 ctcgacgcgg agccgctgcc gacgcgtgcc ccgggtgtag acggcggcca tgcccacccg
     8101 ggccatctcc tcccgctcgt cggggaaccg ctcggggtcg acgacgaacc gggacaccgg
     8161 gttgatcagc gcccacggcc gcacccgcgc acgttcggca gcagcagcgg cgatccggtc
     8221 ggtgtggtgg tcggtcagcg cggccacctc ggcggccagc tcgcggtcgt cgagcaacag
     8281 gtggggccgc gtccagccgg ggatctccgt gccggcatgc gggacgtgca gcagcaccgg
     8341 gctgaggtcg tgacccggaa cgaggatgcc cggcacccac ccatcctgcc cgtcggtgca
     8401 gctgccgcgc tcctcccggt gtgaccgttt cgacgcacgg ttgacggccc cacccgtcgg
     8461 tgctggagtg gaggtcgtga ccatccgggt tcccctggtg acccgaccgc acatcgacct
     8521 cggtcgcgtg tggtcggcgg cctgtccggg ctgctgagcc cgcagctacc agaccgcacc
     8581 cgccatccgg aaggaccacc catgcccgtc gagttcctcg gcatcggctc caccaacaac
     8641 ggctcggaga cagatccgcg caccggtccc gctttcgaca aggagtacac gctcgccctc
     8701 gcccgggcgc acgaggagac cggctgggac cgcgttctca ccgcgtacgg gtcgggcagc
     8761 cccgaccccg cccaggcggc ggcgctgatc gcgacgcaca ccgagcggct gcaactgctg
     8821 ctcgcccacc gtccgaacgt ctcctacccg accttcgccg cgaagacggt cgccaccctc
     8881 gaccagatca gcgacgggcg gctcaccctg cacgtgatca cgggcggcaa cgaccacgaa
     8941 cagcagcgcg aaggcgacac cctgcccaag gaccgccgct acgaccgcac ccgcgaggcc
     9001 atccagatct tcaagaaggc gtggacctcg cggacgccct tcgacttcca cggcgagcac
     9061 tacgacttca ccgacttcgt gctcgacgtc gagccggccc agaagccccg cccgcgcatc
     9121 tccttcggcg ggtcctcccc cgcggcgtac aaggtgggcg cggaggaggc cgacatctac
     9181 gcgctgtggg gggagccgct cgccggcacc gccgaacaga tcgcgacgat caccgagctc
     9241 gccggcgcgg ccgggcggcc ggcgccgacg ttccaggtgg cgttccgccc gatcctcggc
     9301 aggaccgagg aggaggcttg ggagaaggcc tacgcgacgg tggctcgcat ccaggaccgg
     9361 acgaagggcg gcacgtcaca gctcacccgc cggcacaagc tcaccgatcc cgagaacgcc
     9421 ggctcccagc ggctgctgga ggtcgcggcg cagggcgagc ggttcgaccg ggcgctgtgg
     9481 acggtcacgg ctaaggccac cggtggggcg ggcaactcca ccgccctcgt cggcaccccg
     9541 gagacggtgg ccgccgcgct gctggactac tacgacctcg gcgtccggat cctgtccgcc
     9601 cgcggctacg acttgctcga ggacgccaag gagttcggtc gcgaggtgat cccgctggtg
     9661 cgtgccgagg tggccaggcg cgacgcggag caggcccgca ccgccgtccc cgcctgacgc
     9721 cccgcgcacg ctgcgccggg cgggaccggt acgtgaggtc ccgtccggcg gatcgtcatg
     9781 ccagcagcgc tcagcggtgt gagggcgggc gggacggttc cgccccggcg cgcgacggca
     9841 gcgggtcgct acgcacccac tggccctcgg cgtcgcgttc gaagtcgtca agcagcagcc
     9901 gcaccgcgtc gcggttgcgc tgcgaaccga ggtcgacctc ggcgaaacgg cggtcgagca
     9961 tcagctgcgc gagggtgctg cgcggccggg gagcagacgt ctggccgacg agccagtcct
    10021 cgaacatgat cactgcgggg aacctccggg gcgggaacaa ccacttcacg atgccatcgg
    10081 atcgcccgac tgcggcccgc gtgtgacgca gccgtcccgc gtcccgggcg gaactcagcg
    10141 gagcaccgct tgagtctgcg tcacccgcgc cgcgagcttc ccggccgcgt catgcacctc
    10201 cgtgtcgacg acgatcgttc ggcggccctt gtgaagcacc cgggccacgg cgttggcgtg
    10261 cccggacgtc acggcccgca ggaagttcgt cgccgacgag actgtcgccg tgcccgtcgc
    10321 gccatcgggc aggttgagga acgcgcacca gccgcccgcc gcgtccgcca gcgccatcag
    10381 cgcacccccg tgcagcaggc cgccggacgt gcaccgcgtg gcatcccagt cgaggcgcag
    10441 cgccacctcg tccgggccgg cccgcaacgc ggtggccccg agcagggcgg tgaacggcat
    10501 cacggtgtgg agctgagcgg tgaggtcgtc ggccacggtg cacacgctgc cggtcgccgc
    10561 gcggctcgtc catgacctcg gacgtcatcg atcaccgtcg ccgcgaccgc tagcgtcgcc
    10621 ctcatgaccg ccgcaaccgc caccggaacc gcgttcggat cgctgttgcg cgactggcgt
    10681 cagcgacgcc gcctcacaca gctcgacctc ggcctgcagg ccgacgtgtc cgcgcggcac
    10741 ctgagcttcc tggagaccgg ccggtcgaag ccgagccggg agatggtgct gcacctctcg
    10801 gaggagctgg acgtgccgtt gcgcggccgg aacgagctga tgatggccgc cgggttcgtg
    10861 ccggcctaca ccgagcacgg cctcgacgac gccgagctgg gcgaggtgca gcgctcgctg
    10921 cggcggatcc tcgacagcca ggagccctac cccgcggtcc tcgtcgacgg ctcgtggaac
    10981 ctgctcgcgg cgaaccgagc ggtcgcgctg ctcaccgacc tcgtcgaccc cgcgctgctc
    11041 gccgcgccgg cgaacgtgct gcgggtgagc ctgcatccgc gcgggctcgc cccctacgtc
    11101 gtcgatctcg aggagtacgc cgcgcacctg gtgtcccggg tgcgccggca ggcggagcgg
    11161 gccgccacca ccggcctccg cgcgctgctc ggggagctcg tcgggtactg ccgggacgcc
    11221 ggcctcgacc cgacggccca gccccacgac cgcatcgtgc tgcccctgcg gctgcgccac
    11281 ccggaccagg agctggcgct gttctcgacg gtggcggtgc tcggcgcgcc gctcgacgtg
    11341 acgctggagg aagtcgccat cgagagtttc ttccctgccg acgacgcaac ggccgcctat
    11401 ctgcgcgaac ggttcgcctg atccctcccg atcgccccgc ccgtcgtgga tcatggagat
    11461 ccggtcgcgt gacgggggac cgcgtgggag agaagccggg gcctgccccg ctgatcgggg
    11521 tcgacgacat cggcacgccc tcgtgggcgc agggctacga gtcggcgcgg ccgcggcccg
    11581 tggcctgctg gatgccgcag tcgccggtgt gtcgggcgcg ctcgtgccgc gcgtggagca
    11641 gcgggcgcag gacatgctgc atgcctcggt gatcgaggtg gagctcaccg ccgccgcccg
    11701 gctgtcgggc accgacaccg tcgtcgccga tctccccgcc ggctgggcca ccgtgctctc
    11761 ccggatgttc cagaccgggc gcgacctgtc ggggcgggca gtggcagccg cggtcggtgg
    11821 cacgcgggct gtaccgggac gcgcctgtgg tggtggccga cgagccgacc ggcgccctcg
    11881 acgcccgtgc cgagcacgcc gtcttcgcca cgctgcacgg gctcgggggc cggggcgcac
    11941 cgcatcacgg tgctcgtcac ccaccggctg gcgaacgtcc cgccacgccg accagatcct
    12001 cgtgctcgag tggggcaggc tgatcgagca cggccgccac gagcagctga tagccctcgg
    12061 tggcacctac cacgagctgt tcacgctcca ggcccgcgcg tacggcgagt cggcgggaga
    12121 gccggatacg gtcccggcat gaccgacgag ctggtgcacc tcgacgtcgc cgcgggcgtc
    12181 gcgacgatca cgctggactc ccccgccaac cgcaacgcgc tgtccgccca gttgcgccgg
    12241 gagctgctgg cacacctcga ctccgcgatc gcggacgaca cggcgcgggt gatcgtgctc
    12301 agccacaccg gacgcgtgtt ctgcgcaggc atggacctca aggagtcgcg cggggccggg
    12361 gcgggggacc agggcgtcaa cgagttcccc gcgatcctgg aacggatctg ggccgcaccg
    12421 acacccgtgg tcgcgcggct ggccggcccg gcccgggcgg gcggcgtcgg gctggtggcg
    12481 gcctgcgaca tcgccgtggc cgccgaggac gcgacgttcg cgttcagcga ggtccggatc
    12541 ggtgtggtcc cggcgatcat ctcggtgacg gtgctgccgc gactgctgcc gcgcgccgcg
    12601 cacgagctgt tcctcaccgg cgagaccttc gacgccgccc gcgccgcaca gatcggcctg
    12661 gtgaacagcg cggtgcccgg cgtccacctc gacgccgagg tgcaccgcta caccgaggcg
    12721 ctgcggctgg gcgctcccgg cgcgctggcg gccaccaagg agatgctgcg ccgggagcgg
    12781 ccgtcggcca tggccgagca gttcgccgag atgcaggagc tctcggcccg cttcttcgcg
    12841 ggcgaggagg gtcaggaggg catgcgggca ttcatcgaga agcggccgcc ctcctgggca
    12901 gtcgtctagg ccgttccctg cgacgccgag tggcgcaggc cgttgacgat gagcgtcaac
    12961 gaggaccaag acggtgactc agcgctacac cacctgccat ggctacacga gaacagggcc
    13021 tgatacggcg tcaccggtcg gtagggctcc agcgcgggtc gcggcccgac gcggcgagcg
    13081 cccgctcgaa cggcggcgcg tcggcgggca cctgcacctc ggcgccgaac cagccgccgt
    13141 cacgccccga cggcgccatc gcctcgacgc cctcgagcac cacttctccg aggccggacg
    13201 gcacgtcgag cggctgcccg gtcgcgacgg ccaggtccca ggcgtgcacg acgaactcgg
    13261 cggtcatgat cgagccgacc accgcggcgg gcatcgccgt gccggccatg tgcgactcgc
    13321 cctcccaggc cgcaggggag gcccacgcct gcgccgtggc ccggccctgc tcgccgcatg
    13381 ccgccgccca ctcgctctcc gggcgcccgc tgaggaacgg ggtgcggtcg gccgcggtcc
    13441 aggacgggtc ccaggcctcc cgggtggcgc agcggtgggc gagcaggagc ccgaaggcga
    13501 tgtggttcac cacgtcccgc accgcgaagt cggagcacgg ggaggagtcg ccgtaccggg
    13561 cctgctcggc accgctgatc gcggcgaccg cgaggtcgat gctctggctc atgtggtctg
    13621 cgttgtccat gcctcgaatc tgccggatgc cctcccgccg gtattgcacg aacccgacag
    13681 gtgctcagct ccgccgcagc gccaccacgg ggatcgtgcg ctcggttctg gtctcgtggt
    13741 cggcgaagaa cgggtaggtg tccgtgagca tcgcccaggt ctcctcccgc cggtcgccct
    13801 cgagggcctc ggccgcgccc gggtacctct cgccgtcgat ctcgatgtcg acggcggggt
    13861 cgacgacgag gttgaggtac cagtccgggt gctgcggcgc tccgatgttc gaggcgacga
    13921 cgaggagcac gtcgtcgtcg cggtggaaca tcatcggggt ggtgtgaggc cggcccgtcc
    13981 ggcgcccggc ggttgtgagc aggacgagcc ggtcgcggtg cataccctcg accggcccgc
    14041 ccccccggaa ctgctcgatc acccggcggt tggtctccct cacgtccatc accccacgat
    14101 cctcgggcac ggccggtgga tattggacga acgcgacagg cggctcgtcc tcggtggccg
    14161 gttagcctgg tcgggtggac gtgctggaga cggcgcttga cgaggtgctg gaccgggcgc
    14221 tcggcgtact ctcccgcagg ccgctcgtgg cgctgaccgg cgccggtctg tccaccgact
    14281 ccggtatccc ggactaccgc ggccccggtt ccccgcgtcg caccccgatg acctaccagg
    14341 acttcctctc cggggagccc gcccagcgcc gctattgggc gcgcagctac gtgggatggg
    14401 cgcggatggc gcacgcggag cccaacgccg ggcatcgcgc gctcgccgag ctggagcgcg
    14461 tcggcgcgct gcacggcctg atcacgcaga acgtcgacgg gctgcacagc gccgccggca
    14521 gccgggcgtt gatcgacctg cacggccgca tcgccgacgt ggtgtgcctc ggatgcggtc
    14581 gccgcagccg gcgcgacgag ctgcagcgac ggctcgacgc actgaacccg ggcttcgtcg
    14641 acgcggcagg tgccgccgtc gaggccgccc cggacggcga cgcggagctc gccgccaccg
    14701 acgggttccg gctcgcccgg tgcaccggct gcggcggcgt cgtcaagccc gacgtcgtgt
    14761 tcttcgggga gaacgtcccg cgcgggcggg tcgccggcgc ctacgccatg gtcgacgccg
    14821 cgggcgcgct cctcgtcgcc ggcagctcgc tcaccgtgat gtccgggctg cgcttcgtcc
    14881 gccacgcgca caagcgcggc acgcccgtgg tgatcgtcaa ccgcggcgcc acccggggcg
    14941 acgagctggc cgaggtgctg gtcgacgccg gctgctcgga ggtgctcccg atgatcgccg
    15001 cgatcgcggc ggccgccgga taacccgacg cggatcagtc gcgtccggtg ccccgccgga
    15061 cagagacgcg gtccacgacg agatcgacga cgcggtccac gcccgccgca gtaccccagc
    15121 acagcggcag catcaccagc accgccagca cgacctgcat cgggaagggg agctgggtga
    15181 tccacagctc gaccgcgtcc caccagtcgg cgacggcccg catcatgttc ccacccccat
    15241 caccttcgca cccgcatcac gtccacacgg tagtcgggag gcgatgcgac tagtttccca
    15301 gccatgttcg ccgtctacgc cgcagagccc aacgccgatg ctccgctcga ctccctcacc
    15361 gtgggcgagc gccccgagcc cgaggtgccc gacggatggg ttgccgtcac cgtctccgcc
    15421 gcgagcctca acatgcacga cctgtggacg ctgcgcggcg tcggtatcaa gcccgagcag
    15481 ttcccgatga tcctcggctg cgacggggcc gggacactcg aggacggcac cgaggtcgtg
    15541 ctgcactcgg tgatcgggga cgccacgcta cacggtgacg agaccctcga cccgaagcgg
    15601 actctgctca ccgagaagca ccagggcacg ttcgccgaga ccgtcgtcgt cccgaagcgc
    15661 aacgccgtgc ccaagccggc cggtctctcc gccgtgcagg cgtccgtgat gggcacggcc
    15721 tggctcaccg cctaccggat gctgttcgtg aagtcgggcc tgcggcccgg ccagacgatg
    15781 ctcgtacagg gcgcgtccgg cggggtgtcc acggcgctgg tacagctcgg tagcgcagct
    15841 ggcatgcggg tctgggtcac cggccgcacc gaggacaagc gggcggtggc ggagaagctg
    15901 ggcgcccatg cgacgttccc gtcgggcgag cggttgcccg agcgcgtcga tgccgttttc
    15961 gagacggtcg gtgacgccac gtggtcgcac tcgatgcggt cccttaggcc aggtggtgcg
    16021 atcgtcgtct gcgggtcgac gagcggtccg aacccggggg ccgatctaca gcggttgttc
    16081 ttcctccagc tgcgtgtgat cggctcgacc atgggcaccc gcgacgagct ctctgacctg
    16141 ctgagattcg tcgcggaagc cggaatcaag cccgagatcg ggctcgaact gccgatggat
    16201 caggcggaag gaggcttccg cgcaatgctc gaaggggaaa catcgggaaa gatcgtcttt
    16261 acgagatgac accgtccgtc gcgctggtgc tgccgttcgt cgacggtata tcgatcctga
    16321 gagacgcggc aggcggtctc aggacgtaca gtacgtaacc gtgtctgagc caccaagtcg
    16381 aatggcgtcg gggtccacgt accccccggc gccgcgcgtg acaggtctgg tcgagctgct
    16441 cgaccggttg gaccgcgcgg ttgccgccgc gttgtcgccg gtcacctcgg cggaaggggt
    16501 cagccgagac ggctggcgcg tcctactgat gttggcccgt ggaggtggtc ggagcatggg
    16561 cgagatcgcc tcgcacaccg ctctgcccgc gccgacggcc actcgcgtcg tggaccggtt
    16621 ggtcgccggt cggctcgcct accggcgtac cgaccccgag gatcgccgtc gggtcctcgt
    16681 ccacctcgcg ggggatggac gcgccgtggt cgagagggtc tgcgggcgcg tggaacgccg
    16741 cgcaggcgag gctctcggct ccccccacac ccccgagcgc gcccagctcg ccgagctcct
    16801 ggaagccctg gcctgccagg acctcaccgc cctcggctaa ccctcgtccc cgtccccccg
    16861 tcgactcccc gctgtctcct ccccgctgtc tcccccacca accgattggc ccccggatca
    16921 attccgcatc gccccgaggg ccctgtccag ccagtcccgt ccgcgggcac cgaggtgctc
    16981 gcgggcggcc acaaccgccg gtatctcgtc ccgcgccccg gtcagcgccg ccatcaggcc
    17041 ggccaccgtg acgccgtgag ccggacggtc gagcacgcgc acggcgtcgc ctgcccgcac
    17101 ggctgcaggc cgctgcacgg cgagatacgc gtccggccgt cgcgcgtcga cgaaacgccg
    17161 gaccaggcgg gcacgctgcg gaagccggca cagacccggc agggatccgt ggcagcacgg
    17221 gcctcgccga ccgcccagcg ctcgccgagg acggcgcccg agcagtccag cccggacagg
    17281 gtgaggttct cccccacccc gcccggcagc acctgctgcc gaagccctgc cgtccagaac
    17341 gcgaggtcgt cgcacgagta ggcgtagacg gctggtccgg gccgccgtgg tgctcgacgt
    17401 cgcacactgt gtccccgtcg acgccggacg tcgtgagcag taccggaccg tcgaccggtc
    17461 gcctgtcgat gccggagcgc ccgctctaga ccttcgtcca ggggtccgag cgcacgacgg
    17521 ccacgttgac cgactcgacg cgtccggggg tcgacgtcgt catccggaag ttccctctcc
    17581 ctgtggggcg gcggccggag cccggaacag cgtgtcggcg gtgacccggc ccgcggcggc
    17641 atacgggaac gccgcgagca gcaggaccgc tatcccgagc acgaccaggc gccgaaacca
    17701 cgtgccccgc ccacacagcc atccggctgt gaacgccact gcgagcggca cagccaccgt
    17761 gtacagcgcg gtgatcgccg acgcccgatc atcggtgccc gccggttcgc ggagcgcgac
    17821 gatcacaatg ccgagcacga gtaccccgag cagccccgcg agcaacaagg tccagcgccg
    17881 cttcgtccgg ccggcgccca ccccgaccag caggacgacg agaaggacga gcgcagcgac
    17941 gcccggcgac ccaccgccgg ggaccaccgg cagattcgtc atgaccttga tggtaggggg
    18001 cagagcggat catcgggacc cgcagggaag aagccccggc gacgtcggca ggccgagcgg
    18061 acctacttct tcccggcctt ggcgtcgccg gcggagtcgg tggacagcgc tgccacgaag
    18121 gcctcctgcg gcacctcgac ccgtccgatg gtcttcatcc gcttcttgcc ctccttctgc
    18181 ttctccagga gcttgcgctt gcgggtgatg tcgccgccgt agcacttggc gaggacgtcc
    18241 ttgcggatcg cccggatgtt ctcccgcgcg atgatccgcg acccgatcgc cgcctggatc
    18301 ggcacctcga actgctggcg tgggatcagc tcacgcagct tggtggtcat ggaggtgccg
    18361 tacgagtagg cgtcgtcctt gtgccggatc gccgagaacg cgtcgaccgg ctcgccctgg
    18421 agcatgatgt cgaccttgac cagctccgcc tcctgctcgc ccgcctcctc gtagtcaagg
    18481 ctggcgtagc cgcgggtgcg ggacttcagt gcgtcgaaga agtcgaagat gatctccgcg
    18541 aggggcatcg tgtagcgcag ctcgacccgg ctctccgaca ggtagtccat gccgccgagc
    18601 tggccgcgcc tgccctggca cagctccatg atcgtgccga tgaactccga cggcgccagg
    18661 atcgtcacct tcacgaccgg ctcgaagatc tgtgccacct tgccggtcgg ccagtccgac
    18721 gggttggtca ccgtcatctc ggtgccgtcg tccttgatca cccggtagac cacgttcggg
    18781 gcggtcgaga tcaggtcgag gcccgcctcg cgctccagcc gatcgcgggt gatctccagg
    18841 tgcagcaacc cgaggaagcc gcagcggaag ccgaagccga gcgcggccga cgtctccggc
    18901 tcgtaagtga gcgcggcgtc gttgagctgc agcttgtcca gtgcctcgcg caggtcgggg
    18961 tactccgagc cgtcgacggg gtagagaccg gagtacacca tcggtcgcgg ctcgcggtag
    19021 ccggtgagcg gctcggtggc gccgtggcgc tggctcgtga tggtgtcgcc gaccttcgac
    19081 tgccgcacgt ccttcacgcc ggtgatcagg tagccgacct cgccgacgcc gagcccgatg
    19141 ctcggcttgg gctccggcga gacgatgccc acctcgagca gctcgtgggt agtgccggtg
    19201 gacatcatct tgatccgctc gcgcggggtg atcttcccgt cgaccacgcg aatgtaggtg
    19261 accacgccgc ggtaggtgtc gtagaccgag tcgaagatca tcgccctggc cggggcgtcg
    19321 gggtcgccga ccggcgccgg gacctgccgg accacctcgt cgagcagttc gcgcacgccc
    19381 gcgccggtct tggcgctgac ccgcagcacg tcgtccgggt cgcagcccac gatgtgcgcg
    19441 atctcggcgg cgtagcggtc cgggtcggca gcaggcaggt cgatcttgtt gaggaccggg
    19501 atgatcgtga ggtccttctc cagcgccagg tagaggttgg ccagcgtctg cgcctcgatg
    19561 ccctgcgcgg cgtcgaccag caggatcgca ccctcgcacg cctccagcgc ccgcgagacc
    19621 tcgtaggtga agtcgacgtg gccgggggtg tcgatcagat gcaggacgtg ctcggtttca
    19681 cccacctgcc agggcagccg gacgttctgt gccttgatcg tgatgccgcg ctcgcgctcg
    19741 atgtccatcc ggtcgaggta ctgggcacgc atcgcgcggc cctccaccac accggtgagc
    19801 tgcagcatgc ggtcggccag ggtggacttg ccgtggtcga tgtgggcgat gatgcagaag
    19861 ttccggatca gctccggcgg ggtgaacgtc ttgtcggcga acgagctcac tcacgattcc
    19921 attcaggtgc aggtagctcc atcgtcccat gcgctcagcc gacgggctgc tgaccgtccc
    19981 attcgtcgag cggagcgcag tgccggcgcc acacgcagcg ccgcgggagg tcgccgagtg
    20041 cggttcggcc gttgcaccac agcagcgccg cccacgggtc ctcgtcctcc ggcccccacg
    20101 ggaagagccg gtgcagtacg cccgtggcga gcgccgggtc ggggacgaac ggctgccgag
    20161 gccggtcgcg gcgtcggcag tgtgcacgag gatctcgtcg caggccatgg cggcgaagcc
    20221 ggacacgtcg gccggccccg ccaggtgccg gccacgggct cccggcgccg ccgatgcgac
    20281 gacggcggcc agcacggcgg cgtgggtgac gacggtgtgc aggaggcggc tcggctgcgt
    20341 tgccgggcgc acccgcagat ccatcgtgga cagctccacc ggcccggccg cgaggtcggc
    20401 ggcataccac aggagcgtgt cggcgatgtg cgccacgacc tcacagaccg cgaggttgag
    20461 gtcgcctgcc ggaatcgtcc agtcgagctc gagggtgggc gacagtgccg accggcacgc
    20521 agctgcggcc aggcgcacgt cgtcggcgtc cacggcgtga ccgtagcccc actccccggt
    20581 cccgatcggc gggtcaggcg gacggtcacc gagctcaggg gacgaggacg ccggcatggg
    20641 tggcgaagcg cgccaccagg tcgtggcagg cgtcctcggt cgcggcggag cgggcgacga
    20701 gatcggcccg cagggcaggc aggtcgacct ccgcggcgag gacctcggcg accccggact
    20761 cctcccacga cccgacccac gccgcgaacg ccgccggggt gatctcgggg tggaactgca
    20821 cgccgaggtg cggaccgtgg gcgaacgcct ggagcccgac ctcgttgctc gccaactcct
    20881 gcgcccccgg cggcagggtg aaggcgtcgt cgtggaactg caaccactcc cccgcgggca
    20941 gcaggtcggg cgccgcggtg ccgagcggga cgaagccccg ctccgaccgg ggcgcgcgcc
    21001 cgaccgcgcc tccgagcacg cgggccaaag cctgcgcacc gaagcagatg ccgagcaccg
    21061 gcgtgccggt cgcgactgcc tgtcccatca gatcgagctc agcggcgagc catgggacgg
    21121 tgtcgtcggc ggccgactcg ctcgcaccca gcacgacgag caccgcgacc tcggcgagat
    21181 cgggcactcc cgggccgcct ccgacgaggg ttgtgacgag cgggtcgagc ccgagcccgg
    21241 tcagggccgg gccgaccgtg ccgacctcct gctccccgcg accggggaca gcgtcgtgga
    21301 cgagtaccag agccgttccc atgatcccca ttagtactgc acctcggccg ggccacaggt
    21361 gccgtgatgg ttccgcaaca cgctggtcac gcatcggaat cgtcatggac acatgggtca
    21421 tgaggatcgg caacatgact accacgccgc agcctcccct ggatcgcaag caggccgcaa
    21481 ccgccgtgct ggccgccaaa cgccggaccg gggtgcgctg ggccgacctg ggcaagacca
    21541 tcgaccgctc caccgagtgg accaccgccg ccctgctcgg ccagcacccc ctcaccccgg
    21601 agcagaccgc cgcgctcggc gccgtactgg agctggacga cccggtgatc gacgcattgg
    21661 gcctgccacc ggtgcgcgga cgcgacgtcg tcgacacctc cgagcccatc gtctaccggc
    21721 tccaggagat ggtgcaggtc tacggcagca cgatcaccga gctcgtgcgt gaggagttcg
    21781 gcgacggcat cgtctccgcc atcgacttcg agatggacct cgtgcgcgtg cccgacccga
    21841 agggcgaccg cgtccggatc accctgaacg ggaagttcct gccctatcgc acctggtgat
    21901 ccgaacccga ccgcccccgt ggcccgatcc cgtgtcacca tgaaccagat cgcgtggaga
    21961 cgcggggccg gaggagatct cgcgggcccc gggccgggac ccagccccgg cgacgaccga
    22021 ccgagcagcc cgatggcctt ccctgggggc gaccgtgaca ttgtgctcgt ccgtcgcgat
    22081 caaccgcccg agcgagtgct ccctcgtcac cggcgggccg agctgtcgct gcccggagga
    22141 cgccccccgg ctggccgacg ggcagatcgt cgtccgcgcc accgacaagt tccgctggca
    22201 gcagcaggca tcgcccgacg gacgcagtgc cgcgcgacgg ggcctcgacg gcctgctcgc
    22261 ccacgcccgc cgcaacagcc cgtcggtcgt cgtctccgag ctgctccgga tgagcatcct
    22321 ggttcgcatc ctccgcccca accgccccgg cgacgagatc aaggccgagg tcgaggcgct
    22381 gctccaggag tacatggagc tcgcggagct cgacgacgac ccccgcaggc tcggcgaggc
    22441 cgccaccctc cgcgcgcacc gcacggccat gttcgggaag gcggagaacg cgctcgccga
    22501 caccgccgcc gcgcacgcca tcctcaccga cctcacctcc cccggccccg gcgaggaccc
    22561 ggccgaatgg tcccggctgc tcgcccggag tctcaacgtt ctggtgctcg tactgctcaa
    22621 gctcggctcg cacgaactgg cggacgaggt gtcgcagcgc gcgatcgcca tgtccgagaa
    22681 ctgcgcgtcc acaatggacc ggctcgtgta ccagctcaac cgcgttcggc tgcagctgtc
    22741 ctgggcgttt cgtctcgagc gcggcggacg cgaagcggcg gcggccaccc ggttcgtcgg
    22801 cgccgcgcag accgcgcaca ccgccgcgct gctctggggg cccgcgcacg accggcccgc
    22861 aggcggaccg ccggccgtcg aggagtgctc gatcatcggg tcggcatacg cactgaacca
    22921 gccgggaccc gaacacctcg agatgctggc ccgcttgact ccgatggcgt tcttccccga
    22981 tgacctaaag ttgatcgcca tcgccaccgc gcgctgcctg ctcgccgcgg gccggcccgg
    23041 cgacgcagcg gcgtccctcg ccccgttgca ccgggagtta caggccgaca cctcgggcgg
    23101 actcgtcctc gccgtgcacc gggagttcgc catgatcgac gacctcgccc gtggagcacc
    23161 ccggcggtcc gatgccctcg cgagctacac cgcggctctc gaggacgaac tgtgggcgct
    23221 gcgagaggcg cggctcacag cactgcgcag ccactccgag caccaccggc tgacccgcga
    23281 gcacggcgcc gtcgccgcac aggccctgca ggacccgctc accggcctgc cgaaccggcg
    23341 ggcgctcgac ctgcgcctcc tcgaggtgac cacgtcgtcg gcctgccagc cgtgcgccgt
    23401 cgcgctgatc gacctcgacg gcttcaagga cgtcaacgat gcgcgctcgc acgccgcagg
    23461 cgacaccgtc ctccgcgagg tggcggcgtg cctgcgcacg atcctgcgtt cgcaggacct
    23521 ggttgcccga tacggcggcg acgagttcgt cgtggtcatg ccggccaccc cgctcgcgga
    23581 ggcgcgcgcg gcgttgcagc gggccgccga cgccgtggcg gcgctgcccg ccgacgtcgc
    23641 agccggcgtc acgatgtccg tgggggtggt ccgcgccccg ctcgacagcg agcccgctgc
    23701 ggcgctggcg gccgccgacg ccgcgatgta ccgggcgaag cactcgggcg gcaacaccgt
    23761 gatcagcggc ccgggcgcgg atacgagtgc aggggcactc gtatccactc accacgtggt
    23821 cggccgccag ggcgtcacaa cgagtcccag aaccgcgtga gcgccgccgc gccgcgatcg
    23881 gggtcctggg tcatccacca gtgtccgagc ccgtcgagca cctcgaggcg agcgcctgcc
    23941 cgggccgcgg cacgacgccg ttgctcgtcg gtacccacga agtgatcctc cgtcgggagc
    24001 agcgctagac cggggcgggc ggctgcggcc tccagcccgg tgccgagctc ggccatcacc
    24061 ggctgggcgg cggagcggta gagcgccagg atgcactccc ccatcacagc gtcgaacccc
    24121 gccgcgacgc gcgcggccac cgcgggagtc atgccgcggt ccgccagggc agccgcggcc
    24181 tgctccaccg ggatggccag gcgctgcgcg atcgccgtct caccgacgcc gggggtctgc
    24241 cagccctgcg cgagctcgtg ccacacgtac tccgggtcgt agaccccgag gatgtccgag
    24301 caccaggtgc ggagcaggtc aggacgcgtc atcgcaacgt tgagcacgtg cccgccgccc
    24361 cagtcgtggc cgaccaggtc gaccgggcgc ccgatctcct ccagctcatc caccaaccag
    24421 tcgcggtact cgtcgaccgt tgccgtccaa ccctccggga ccggggcacc gaaaccgggc
    24481 ggcgacagcc gcacctggtc gtcgtagccg agcgcgccga ggcgctcgct cagcaggtcc
    24541 cacaccgccg aggtctcagg attgccatgc acgagaacca ctgtcacgtg accaccttat
    24601 gcacgccgca ctgcccggcg accggggccg attaacgtct ggtcctgtcc gggctgtagc
    24661 attgaccgct gagcgcccgg cgtgaacgca ggatcccccg ccgccggtgc ggaccggaac
    24721 aaggcgaaga agaactcgag gcattcgcgt ggccaacatc aagtcccaga tcaagcgggt
    24781 caagaccaac gagaagcgcc gtcagcgcaa caagtcggtc aagtccgccg tccgcacggc
    24841 cgtgcggcgt ttccgcgagg ctgcggaggc cggcgacacc gagaaggcca ccgagctgca
    24901 gcgtgccgcg tcccgggcgc tggacaaggc ggccagcaag ggcgtcatcc acaagaacca
    24961 ggccgcgaac cgcaagtcgt cgctgtccaa gcgcgtcggc gcgctctgat ccggctccac
    25021 atccggccct gacggcgctg cccctccggg gcggcgccgt tgcgctgtgt ccggaagtgc
    25081 ttgcccggcc tcacaccgcg cgtgactcgc gcgccgcgca tatccgcagc accgctcgct
    25141 ccaaggcgta gccggggtcg gcggccgcac ccttgacgtc ggcattcacc tgggcagcgg
    25201 cgtcgaacgc gacggcaagg gactccgggc gccaggcccg tacctgcgcc tgcgctttgc
    25261 gtaccttcca cggcggcatc ccgagtgttc ccccgagccg gttcgggtcg ccccgacccg
    25321 ctgcgcccac cctggccagc gtgcgcaacc cgtcggccag cgcatcggcc accagaaccg
    25381 gcgccacacc gagcacgagc gcccaccgca gcgcctccag cgccccgggc cggtcgcctg
    25441 ccatggcctt gtctgccacc gcgaacccgg acgcctccgc gcgcccccgg tggtagcgac
    25501 gcaccgcccg ttcgtcgacg gtgccaccgg tatcggcgac cagctggttc gtcgcggcgg
    25561 ccagctcccg caggtcggat ccgacggcct cgatcaggac gccgacggcg tccggcgcga
    25621 tccgcccgcc tgcccggcgc acctcggcgc gcacgaaatc ggaccgctcc tcgaagcggg
    25681 tgatccgatt gcacgtggtg acgacggcgc cgcccttgcg cagcgcgtcg gccagcgcct
    25741 tgttgcgcgc gccccccgcg tgcagcacga ccagcacgat gccctccgcc ggatcggcgg
    25801 actgttcgac gatggcagcg gcgagctcct tgccgacctc gtgtgcggcc tgcaggacga
    25861 tcacccggcc ctcggagaac agcgacgggc tgaggttgtc ggccaggtcg accggagtca
    25921 gctcggcggc ccggtaccgg cgcagctcgg cgcccggctc gaccgccctg acctccgcga
    25981 cgatctgctc gaccgcgcgc tccaccagca gctcctcctc accgaggacg aggcgcagcg
    26041 gcgcgggtgg aggtgacgcg gacatgcctc ccatgctcgc gcacggcacc gacatcgcgt
    26101 gacccggctc ccagatcatg agcagagttt gggtctgcgg ccgccggtgc cgtaacgtga
    26161 aggtcatccg caggcccctg tgccgcggca caactgaaca tggctcatcc agaggggcag
    26221 agggaacggc ccgttgaagc cccggcaacc ggcgaagcgc gcatcacgcg cctcgatcac
    26281 ggtgccaatt ccgacccgca tagtgcgggg aagatgagga gatcttcaat gacccttact
    26341 gccgatgtct cggcatccac gtcctacgac ctcgggccgg cgcgcgcctt gtcctgtcgc
    26401 gagtgctccc accagatccc gctggccgcc gagttcgcct gcccgcagtg cttcgggccc
    26461 ctcgaggtcg cctacgactt cggcaccgtc acgcgggagt cgattgaggc cgggccgaag
    26521 tcgatctggc gttaccagca gctgctcccc gtgccgtcca cagtccggga gcacgccaac
    26581 accgagccag ggctcacccg cctgatcaag gccgaccgcc tgggcgtcgc gctgggcctg
    26641 cgcaacctct gggtcaagga cgacacgggc aacccgaccc actcgttcaa ggaccgcgtg
    26701 gtggccgtgg cgctcgccgc cgcccgtgag ctcgggttct cggtgctcgc gtgcccctcg
    26761 acgggcaacc tcgccaacgc cgtggccgcc gccgccgccc gggccgggtg ggactccatc
    26821 gtgctgatcc cgtcgtccct cgagcaggcc aaggtgctca ccaccgccgt gtacgacggc
    26881 acgctgctcg ccgtggacgg caactacgac gacgtcaacc ggctggccac cgagctcgcc
    26941 gccgagcacg aggactgggc gttcgtcaac gtcaacgttc ggccctacta cgccgagggc
    27001 tccaagaccc tcggctacga ggtggctgag cagctcggct ggcggctccc cgagcagatc
    27061 gtcgtgcccg tcgcatccgg gtcgcagctg accaaggtcg acaagggctt caccgagctc
    27121 ggatcgctgg gacttgtgga gcccacgccg taccgggtgt tcggggcaca agccaccggc
    27181 tgctcgcccg tggcgaaggc cttcgaggac ggccacgacg tcgtgcagcc cgtccgcccc
    27241 gacacgatcg cccgctcgct cgcgatcggc aacccggccg acgggcccta tgtcctcgac
    27301 acggtccgcc gcaccggggg cgcgatggcg cacgtgagcg acgctgaggt ggtggacggc
    27361 atccggctgc tcgcccgcac cgagggcgtc ttcgccgaga ccgcaggcgg ggtgacggtc
    27421 gccaccgcga agaagctcgt cgagacgggg aagctcgacc cggatgccga gaccgtgctg
    27481 ctgatcaccg gcgacggtct caagaccctc gacgcggtgg cgggccagat cggcccgtcg
    27541 gccacggtgc cctcgaccag caaggccgtg cgcgaggcac tggcggcccg cgggctctga
    27601 gaggactgcc gggctcgccg cttccgtgac cgctgcgggc agccgccccc cgcggtgtca
    27661 cgcacgtgcc agatcagcga agtacccgcc tgctctccgt cgccggctag ctcggccggc
    27721 gacgggcgta cgccagcgag gtcggctcgg cgctccccgg ccaggttccg gtgagggtct
    27781 cgacatcgcc gtcgacgtgg gtcagcccgc cgaggacgcg ccgcaccacg agctcgagcc
    27841 cggacgcacc gaccgtcgtg gtggtggcgt cgtccacggc cgacaccgag tcgaccgagg
    27901 gagcctcgac gcccggcgta ccggagccga ccacccggac ggcgggctcc cggcgctcac
    27961 tccggccgtc cgcctgctcc acccactcct cggcctcccg gccgccggcg aggatcgccg
    28021 tcgcgaggac ggcgccgtac acggggtcgc cgcacccgtc gtagatccac ctgcgcccga
    28081 gcacggagtg ctccgtggtg ccgacgagga aatcctcggc gcccgccagc ggcgcgccgc
    28141 ggtaggtcag cggcacctgg agcacctgcg cgtcgccggt ccgcaccagg tgcgtctcga
    28201 tccccaccgc accggacggg tcgtcgaacc ggtaggcacc gatcagctcg gcctgccctg
    28261 ccggggcgtt cctcgaccac ggccgcgacg gtagccattc ggcgatgagc tcacgcttcg
    28321 tcggggtcag ctgcgcacga tggatcaagg acatggagcg gaaactacgt cattagtgcg
    28381 cgagagccgc gagcggcgca gatgtcactc cggcttccac cgggagaacg cctcctggac
    28441 ctcgtggggg gcgtggtcca gggcgtcctg gtcgcgtttc ggccggcgtg acgtggggga
    28501 agcggacgat gacgtcgtag gcgcgcagct ggtccagctc ggagtagaag ctgccgaaac
    28561 gctgctggga caccgcccgg agggtgcaga ggtcggccgc aggcagggta cgcaggtcgg
    28621 tcgccaacgg ccggtggtgg cttgcggccg tgagcgcatc gaggctgccc ggccgcggcg
    28681 cctccgccag ttccgtcatc agggcgcctg cctgg
//
LOCUS       DASRSC010000199         5241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2691454,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000199 DASRSC010000000
VERSION     DASRSC010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2691454"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="VFE39_09900"
     CDS             complement(<1..1072)
                     /locus_tag="VFE39_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HET6258951.1"
                     /translation="MFLPPDLPDLPVRGVLHEVTGTLADRGTAVLVAPPGTGKTTLVP
                     LALAAGLGTGGKVLVAEPRRLAARAAAARMAALVGEPVGETVGYAVRGDSRRSAATRI
                     EVVTSGLLLRRLIADPELAGVGVVLLDECHERHLDADLLLAMLLDARDGLRPDLQLLA
                     TSATVATGRLAELLGNAGGDAPVLRVEARTFPVAVRHTPPARGERIEACVARAVRAAL
                     NHDDGDVLAFLPGVAEIRRTAAALSGVAADVLPLHGRMPVDEQDRALRPGARRRVVLA
                     TAVAESSLTVPGVRVVVDGGQARVPRVDHRRGLSGLVTARVSAAVAEQRAGRAGREAA
                     GRVYRCWPEGELLARYPEPEIRT"
     gene            complement(1108..2058)
                     /locus_tag="VFE39_09905"
     CDS             complement(1108..2058)
                     /locus_tag="VFE39_09905"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6258952.1"
                     /translation="MSGRPVALVTGASRGLGFLLARELADRGHDLVVNARSEDGLAIS
                     AAELQGRGVSEVLAVPGDVSVEADARRLVAAACERFGRIDVLVANAGIIQVGPASAMR
                     MPDYADAMNVMFWGVLHPTLAALPVMRELGGGRILVVTSVGGKLPAPHLLPYTAAKHA
                     AVGFAEGLQVEAGRYGVTVTTAVPGLMRTGSPRNALFTGDRVAEHRWFTLADSIPLLS
                     MDAERAAAKLVRATLRGRPEVVLTPAAKIGVRLHGLAPATTLRLLAAANRLLPTDETA
                     RPVQPGHAVARPGRWFDTLTGLTRRAAQRYHQHDDAAAPR"
     gene            complement(2055..3107)
                     /locus_tag="VFE39_09910"
     CDS             complement(2055..3107)
                     /locus_tag="VFE39_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HET6258953.1"
                     /translation="MTTVLRTSRPRLVEPPALPEPRGPLSAAVIGSLRRGPEILPPVE
                     VTEPYGDDLQLALYCLYELHYRGFAGVEDDLEWEPSLLTVRAAMERVFLDALHDDVSP
                     SDGSIDAVDAELESLLVEPLEARGVSHHLRREGERWQLREYVAHRSLYHLKEADPQAW
                     VIARLDGPAKAALVTVEHDEYGAGNPDRMHAHLFAEMMLALALDASYGAYLDATPAQT
                     LAEVNLMSLCGLHRALRGALIGQFAMVELTSSPGSDRLVRAMRRLDLPDAAVEFYAEH
                     IEADAVHEQLVRRGVIAPLLAAEPALARDIVFGIRASGFLAERFGDLLLERWAEGRSA
                     LRHPLPDAPPIRPAAS"
     gene            complement(3104..3283)
                     /locus_tag="VFE39_09915"
     CDS             complement(3104..3283)
                     /locus_tag="VFE39_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HET6258954.1"
                     /translation="MLTDEGPLLVSGPVEVVLPDGSRVRSDRPVSALCMCRRSRRQPF
                     CDTSHRRKVRQDEGG"
     gene            complement(3315..3965)
                     /locus_tag="VFE39_09920"
     CDS             complement(3315..3965)
                     /locus_tag="VFE39_09920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HET6258955.1"
                     /translation="MLLRPPGVYRAEGDTQMLTDVMRQGGFAIGREVLDIGTGSGALA
                     VSAARAGARSVTAVDLSLRSVVATWLNSRLHGAAVSVHRGDLFVPVAGRRFGLVVANP
                     PYVPAPTETLPRHRMSRCWDAGPDGRALLDRICAGVTDVLAPGGDVLLVHSAVCDTAM
                     TLDALARAGLVAQVLSRATVPFGPVMRRRAALLEARGMIRPGQSFEELAVVGARHG"
     gene            complement(4008..5084)
                     /locus_tag="VFE39_09925"
     CDS             complement(4008..5084)
                     /locus_tag="VFE39_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET6258956.1"
                     /translation="MTTAAAGTPEAPSRPRRGLTATMANTSTATTQTTETNVVTDTIV
                     AQLRALEQLTQTEAQIARLRIAQARTDAVRRELQQNGDNAVRRARRIADQLRALDAVP
                     DVVTPALGRVLALVKSMVEQAQPIDEALLGDLSLEHQLLDRARYVRVLADRAELPAVE
                     RLADDLVTAHSATVDWLTTVLAEEALGGPAALTPTPLQRVAGGVVHAVSLPTRFAVAR
                     VNRAVHIAYRSGEQARDALGDVAGKVVQLGSGTREVAAAGRDAALQRAERVAAREGAG
                     GVAGVVHETRADLGSLKASELPIQHYEEMTAQRAIAALRELTSPADLTVMIAFEESHK
                     NRSGVISAAQTRYASIAQNAAGVE"
BASE COUNT          688 a         1933 c         1951 g          669 t
ORIGIN      
        1 cggtgcggat ctccggttcc gggtagcgtg ccagcagctc accctctggc cagcagcggt
       61 agacgcgccc ggctgcctcc cggcccgccc ggccggcccg ctgctcggca acggcggccg
      121 acacacgtgc ggtgacgagc cccgacaggc cgcggcgatg gtccacacgc ggcaccctgg
      181 cctgcccccc gtccacgacg acccggacgc cgggcacggt caggctggac tccgccacgg
      241 ccgtcgcgag caccacccgg cgccgggcgc ccggccgcag agcccgatcc tgctcgtcga
      301 ccggcatccg gccgtgcagc ggcaacacgt ccgcggcgac gccggacagg gcagcggcgg
      361 tgcgccggat ctcggcgacg ccgggcagga aagcgaggac gtcgccgtcg tcgtggttga
      421 gcgcggcgcg cacggccctg gccacgcacg cctcgatccg ctcgccgcgg gccggtggtg
      481 tgtgccgcac cgcgacgggg aacgtcctcg cctccacccg aagcaccggg gcgtcgccgc
      541 cggcattgcc gagcagttcg gccagccgcc cggtggcgac ggtggccgat gtggcgagca
      601 gctgcaggtc gggccgcagc ccgtcgcggg cgtcgagcag catggcgagc agcaggtcgg
      661 cgtcgaggtg ccgttcgtgg cactcgtcca gcagcaccac gcccaccccg gcgagctccg
      721 gatcagcgat cagcctgcgc agcagcagcc cggacgtcac gacctcgatg cgcgtggccg
      781 ccgaccggcg cgagtccccg cgcaccgcgt acccgacggt ttcgccgacc ggctcaccca
      841 cgagcgccgc catccgtgcg gcagcagccc gggccgcaag cctgcgcggt tcggcgacca
      901 gcaccttccc gcccgtcccg agccccgcgg ccagcgcgag cgggacgagc gtggtctttc
      961 cggtgccggg cggcgcgacg agcaccgcgg tgccccggtc ggccagcgtg cccgtcacct
     1021 cgtggaggac gccgcggacc ggcaggtcgg gcaggtcggg tggcaggaac atctccggca
     1081 gtctgcccgt gcccggtacg ggacggttca gcgtggggcg gccgcgtcgt cgtgctggtg
     1141 gtaccgctgc gcagcacgtc gggtgagacc ggtgagcgtg tcgaaccagc ggccgggacg
     1201 cgcgacggcg tgtcccggtt gcaccggacg agcggtctcg tccgtcggca gcagccggtt
     1261 ggccgcggcg agcagccgca gcgtcgtggc cggggcgagc ccgtgtagcc gcacgccgat
     1321 cttcgcggcc ggggtgagca ccacctcggg acgcccgcgc agcgtggcgc gcacgagctt
     1381 cgccgcggcg cgctccgcgt ccatggacag cagagggatg ctgtcggcga gggtgaacca
     1441 ccggtgctcg gcgacccggt ccccggtgaa gagggcgttg cgtggcgagc cggtgcgcat
     1501 cagcccgggc accgccgtcg tcacggtcac cccgtaccgt cccgcctcga cctgcaggcc
     1561 ctcggcgaac cccacggccg cgtgtttggc tgcggtgtag ggcagcaggt gtggggccgg
     1621 cagcttcccg cccaccgacg tgacgaccag gatccggccc ccgccgagct cgcgcatcac
     1681 cggcagcgcg gccagggtcg ggtgcagcac cccccagaac atgacgttca tcgcgtcggc
     1741 gtagtcgggc atgcgcatcg cggacgccgg cccgacctgg ataatgccgg cgttggcaac
     1801 cagcacgtcg atccggccga agcgctcgca ggccgcggcc accagccgcc gggcgtcggc
     1861 ctcgaccgac acgtcaccgg gaaccgcgag gacctccgag accccgcggc cctgcagctc
     1921 ggcggccgag atggcgaggc cgtcctcgga ccgggcgttc acgacgaggt cgtggccgcg
     1981 gtcggccagc tcgcgggcca gcaggaagcc caggccgcgg ctcgcgccgg tgacgagggc
     2041 gacggggcgg ccgctcatga cgccgccggc cggatcgggg gtgcgtccgg aagcgggtgg
     2101 cgcagggccg agcggccctc cgcccagcgc tccagcagca ggtcgccgaa ccgttcggca
     2161 aggaacccgc tcgcgcggat gccgaacacg atgtccctgg ccaacgccgg ttccgcggcc
     2221 aggagcgggg cgatcacccc gcgccggacg agctgctcgt gcacggcgtc ggcctcgatg
     2281 tgctcggcgt agaactcgac ggccgcgtcg ggcaggtcga ggcggcgcat cgcgcggacg
     2341 agccggtccg agccggggga ggacgtcagc tcgaccatgg cgaactgccc gatcaacgcg
     2401 ccgcggaggg cccggtgcag cccgcacaac gacatcaggt tcacctcggc cagcgtctgc
     2461 gcgggcgtcg cgtccaggta ggcgccatag gaggcgtcga gggccagcgc cagcatcatc
     2521 tcggcgaaca ggtgcgcgtg catgcgatcc gggttgcctg cgccgtactc gtcgtgttcg
     2581 acggtcacca gcgcggcctt cgccgggccg tccagccggg cgatcaccca cgcctggggg
     2641 tcggcctcct tgaggtggta gagggaacgg tgcgccacgt actcacgcag ctgccagcgc
     2701 tcgccctcgc ggcgcaggtg atgggagacg ccgcgggctt ccagcggctc gacgagcaac
     2761 gactccagct cggcgtcaac ggcgtcgatg gagccgtcgg agggggacac gtcgtcgtgc
     2821 agcgcgtcga ggaagacccg ctccatcgcc gcgcggacgg tcagcagtga tggctcccac
     2881 tcgaggtcgt cctcgacacc ggcgaagccc cggtagtgca gctcgtagag gcagtagagc
     2941 gcgagctgca ggtcgtcgcc gtagggctcg gtgacctcga ccggcggcag gatctccggt
     3001 ccccggcgca gcgagccgat cacggccgcc gacaggggcc cgcggggttc cggcagcgcg
     3061 ggcggctcaa cgagtcgggg ccggctcgtg cggagcactg tcgtcatccc ccttcgtcct
     3121 ggcgcacctt gcgccggtgg ctggtgtcgc agaacggctg gcggcggctg cggcggcaca
     3181 tgcacagcgc actgaccggg cggtcggagc gcacgcggct cccgtccggc agcacgacct
     3241 cgacgggccc ggacaccagc agcggcccct catccgtcag catgatctcg cgggtccgag
     3301 gacaccgtgc gggatcagcc atgccgggct ccgaccaccg cgagctcctc gaagctctga
     3361 ccggggcgga tcatgccgcg cgcctcgagc agcgcagcgc gtcggcgcat cacggggccg
     3421 aacggcacgg tcgcacggga gagcacctgt gccacgagcc cggcccgcgc caaggcgtcg
     3481 agtgtcatgg cggtgtcgca caccgccgag tgcacgagca ggacgtcgcc gccgggggcg
     3541 aggacgtcgg tcacgcccgc gcagatccgg tcgagcaggg ccctgccgtc cggtcccgcg
     3601 tcccagcagc gggacatgcg gtggcggggc agcgtctcgg tcggggcggg cacgtagggc
     3661 gggttcgcca ccaccaggcc gaagcggcgc ccggccaccg ggacgaagag gtcaccgcgg
     3721 tggaccgaga ccgccgcgcc gtgcagcctg ctgttgagcc acgtggcgac caccgatcgc
     3781 agcgacaggt cgacggctgt gaccgagcgg gcaccggcgc gggcagccga aacggccagc
     3841 gccccgctcc cggtcccgat gtcgaggacc tcgcgaccga tggcgaaacc gccctgtcgc
     3901 atcacgtcgg tcagcatctg ggtgtcaccc tcggcccggt agaccccggg tggccgcagc
     3961 agcatcacac tggacctgtg cccgctgctc ggtaacccaa acgcaactta ttcgacgccg
     4021 gcggcgttct gcgcaatcga ggcgtagcgc gtctgcgcgg ccgagatgac gcccgagcgg
     4081 ttcttgtggc tttcctcgaa cgcgatcatc accgtgaggt ccgcgggcga cgtcagctcg
     4141 cggagcgcgg cgatcgcgcg ctgcgccgtc atctcctcgt agtgctggat gggcagctcg
     4201 gacgccttca ggctgcccag gtcggcccgt gtctcgtgca ccacgcccgc gacgccgccc
     4261 gcgccctctc gtgcggccac ccgctcggcc cgctgcagcg cggcgtcgcg accggccgcg
     4321 gcgacctcac gggtgccgct cccgagctgc acgaccttgc cggccacgtc gccgagggcg
     4381 tcacgcgcct gctcgccgct gcggtacgcg atgtgcacgg cgcggttgac cctcgcgaca
     4441 gcgaaccgcg tcggcaggct gaccgcgtgc accacgccgc cggccacccg ctgcagcgga
     4501 gtgggcgtca gcgcggcggg tccgcccagt gcctcctcgg cgagcacggt cgtgagccag
     4561 tcgacggtgg cgctgtgcgc ggtgacgagg tcgtcggcga gcctctcaac ggccggcagc
     4621 tccgcccgat cggccagcac gcgcacgtag cgggcgcggt cgagcagttg gtgctcgagg
     4681 ctgaggtcgc cgagcagcgc ctcgtcgatc ggctgggcct gctccaccat gctcttgacg
     4741 agcgcgagca cccgtcccag cgctggtgtc accacgtcgg gcacggcgtc gagcgcccgc
     4801 agctggtcgg cgatgcgacg ggcgcgccgc accgcgttgt cgccgttctg ttgcagctcg
     4861 cgccggacgg cgtcggtgcg ggcctgggcg atgcggagcc tggcgatctg ggcctcggtc
     4921 tgcgtgagct gctcgagcgc gcggagctgc gcgacgatcg tgtcggtcac gacgttcgtc
     4981 tcggtcgtct gtgttgtcgc ggtgctggtg ttcgccatgg tggcggtcag tcctctccgt
     5041 ggtcgggacg gtgcctccgg ggtgcccgct gcagcggtgg tcacgcatcg ccaccatgtg
     5101 accacgctct cgggtgcttt cgtgatcggg tcgcctgcag gcccttcaag cctcgtcgag
     5161 gcgagcccgc gccctggcgg tgcgggtggc cgcggcaccc gccgccctgg ccgaggactt
     5221 cgcggtggcc taggcatcgc g
//
LOCUS       DASRSC010000200         5241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3837695,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000200 DASRSC010000000
VERSION     DASRSC010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3837695"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..1068)
                     /locus_tag="VFE39_09930"
     CDS             complement(256..1068)
                     /locus_tag="VFE39_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HET6258957.1"
                     /translation="MTDEQQRVSISVTAGVADVRLNRPEKRNALDPAMFAGLVEAGER
                     LRSDVSVRAVVLSGEGPDFCAGLDFTSFRAMTSGERLSATAQLPSSAGPAKAAGQRAA
                     YVWAELPVPVIAAMRGNALGGGLQIALGADIRIVAPDARLSVMEVNWGLVPDMTGTQL
                     LPELVGRDVAKELTWTGRVVSGDEAAALGLCTRTDPDPRSAALALAAEIAARSPHAVR
                     AAKRLLDLAGRVDLEAGFAAEQTEIGALIGSPNQVEAVTARFEKRDPSFEDC"
     gene            complement(1086..1859)
                     /locus_tag="VFE39_09935"
     CDS             complement(1086..1859)
                     /locus_tag="VFE39_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET6258958.1"
                     /translation="MREIRYEVTDRVATLTIDRPEKRGAMTYAMFDEFSADVARASAD
                     PDVTVLLVTGVPGSFCSGIDLADLASRSPDDRGRRDGDGRADSLLMGCPKPVVAAVDG
                     MAVGMGAEFATQADLRVISTRARFSWNFVHRGLVSDTGAGTWLLPRQIGVGPALRLLY
                     TGADLGADEALALGFATAVVEPDALPQAALELASTIATASPFALVRTKKLVLDGAGAR
                     LRAHVDATRAAMAECFASDDHAEGVAAFLERRPARFTGR"
     gene            1930..3189
                     /locus_tag="VFE39_09940"
     CDS             1930..3189
                     /locus_tag="VFE39_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6258959.1"
                     /translation="MDFELPVELTQRLADLDDFIEREIRPLQAQDDNERFFDHRREYS
                     RTDFENGGVPRREWEELLGEMFRRADAAGWLRYGLPKEVGGSDGTNLDMAVIREHLAH
                     KGLGLHNDLQNEASIVGNFPFVHMMLQYGSQAQKEEFLEPMLTRTKRIGFGLTEPEHG
                     SDATWMETTAVRDGDDWVLNGAKRFNSGMHSATHDVIFARTSGKPGDPRGITAFFTPV
                     DSPGFSVDFHWWTFNMPTDHAEVTIRDVRVPSSAIFGEEGAGLAVAQTFVHENRIRQA
                     ASGVGAGQYCVDRSVEYARKRVTFGQPLATRQAIQWPLVELQTEAEMLRGLVRKTAWE
                     LDRVPHMQISDKVSMCNYRANRFVCEAADRAMQVHGGLGYTRHTPFEHIYRHHRRYRI
                     TEGSEEVQMRKVAGYLFGFAGPRKGQG"
     gene            3195..3665
                     /locus_tag="VFE39_09945"
     CDS             3195..3665
                     /locus_tag="VFE39_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258960.1"
                     /translation="MDDFRELGGRLSNWGRWGVDGRRDERGTTDLLTPERIVAAASLV
                     RDGKVFDLGIPFGPDGPQPGGGRINPVQLLSETGADQDFPGAFHYADDYVFMPLQSAS
                     QWDGLEELIAPVGRAALGELLEVEDLAQRHSHVPAPPIKFRRAVGSPINPLAIK"
     gene            3731..4378
                     /locus_tag="VFE39_09950"
     CDS             3731..4378
                     /locus_tag="VFE39_09950"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6258961.1"
                     /translation="MARPALPEPGVRRAGPTPARVPHGAVGEAGILDAALAVMAEHGF
                     HGTSVRDIAVRAGVSPAALYHHFASKQQVLATIMERGIKALLERTHAALADAGDEPAD
                     RLRALVEVHVLFHLQDQRGTLLGTSELRALEEPVRSRHLAKRHQQQRLFDGVITQGVD
                     LGVFTTPIPLEASRAIVVMCTGVANWFSPKGRMSRAEVVRRYQRLALDMVGAHDA"
     gene            4401..>5241
                     /locus_tag="VFE39_09955"
     CDS             4401..>5241
                     /locus_tag="VFE39_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET6258962.1"
                     /translation="MTAAATLDGRPVDAGDPDVLARLLARRLTTVTGAEVSVERLTRL
                     TGGASRETWSFAALTDDGHRRLVLRRDPPGPGRPEGMAREAAAVAAAHAAKVPVPLLV
                     DSGTDPAVLGSPYLVTEYVDGETIARRLLREPQYASARESMATELGRTLARIHSVPLV
                     AVPGLETHDPLEHLLASYDALGEPLPTIEIALRWLERRRPDPVAETVVHGDFRTGNLI
                     IAPDGLRAVLDWEVVHRGDPREDLGWLCVKCWRFGSALPAGGFGTVDQLLDGYMEVAG
                     TRPD"
BASE COUNT          743 a         1840 c         1906 g          752 t
ORIGIN      
        1 gggctgactg ttcttcggcc tgtgtcccgg ccatcgccgt cggggtgagt ggctggagag
       61 gcctgcttcg cccgaagtcg gtgggccgtc tcctcaggcc cgtgctggtc ctcgtcgagg
      121 aggttgggtg tcgcgtcgag atgttccgcc ggtccggtcg tgatcgcgat tgatccgcac
      181 aagcggtcat ggaccgcggt ggcggtgaac gcttcgttgc agctgcagac ctcggtccgg
      241 gtcgacgccg accggtcagc agtcctcgaa gctcgggtca cgcttctcga atcgggccgt
      301 cacggcctcc acctggttcg ggctgccgat cagcgcgccg atctccgtct gctccgctgc
      361 gaacccggcc tcgaggtcca cccggccggc caggtcgagc agtcgcttcg ccgcccgcac
      421 cgcgtgcggg ctgcgggcgg cgatctctgc cgccagggcg agggcggcgc tgcgcggatc
      481 ggggtcggtg cgtgtgcaca acccgagcgc ggcggcctcg tcgccgctga ccacgcggcc
      541 ggtccaggtc agctccttgg cgacgtcgcg gccgacgagc tcgggcagca gttgggtgcc
      601 cgtcatgtcg ggaaccagcc cccagttgac ctccatcacc gacaggcgcg cgtcgggcgc
      661 cacgatgcgg atgtcagcgc cgagggcgat ctgcaggccg ccgcccagcg cgtttccgcg
      721 catcgcggca atcacgggga cgggcagctc tgcccatacg tacgcggccc gctggcctgc
      781 cgccttggcc ggcccggcgg acgacggaag ctgcgcggtc gccgagagtc gttcgcccga
      841 cgtcatcgcg cggaacgagg tgaagtcgag gcctgcgcag aagtcgggcc cctcgccgga
      901 gagcacgacc gcgcgtaccg agacgtcgga cctgagccgc tcgcccgcct cgacgaggcc
      961 cgcgaacatc gcggggtcga gcgcgttgcg cttctccggg cggttcaggc gcacgtcggc
     1021 gaccccggcg gtcacgctga tggacacccg ttgctgctcg tcggtcatgc ggtctgctcc
     1081 tgatctcatc gtccggtgaa cctggccggg cgccgctcga ggaacgcggc cactccctct
     1141 gcatgatcgt cggacgcgaa gcactcggcc atggccgccc gggtggcgtc gacgtgcgcc
     1201 cggaggcggg cgcctgcgcc gtcgagcacc agcttcttcg tcctgaccag cgcgaacggt
     1261 gaggcggtcg cgatcgtgct ggccagttcg agcgcggcct gcggcagcgc gtcgggctcg
     1321 acgaccgcgg tggcgaagcc gagcgccagc gcttcgtcgg ccccgaggtc ggcacccgtg
     1381 tagagcagcc gcagcgcagg ccccacgccg atctggcggg gtagcagcca cgtccctgcg
     1441 ccggtgtcgg acaccaggcc gcgatgcacg aagttccagc tgaaccgtgc ccgggtggag
     1501 atcactcgca ggtcggcctg ggtggcgaac tccgctccca tgcctacggc catgccgtct
     1561 acggcggcga cgacgggttt cgggcagccc atgagcagcg aatcggcgcg cccgtccccg
     1621 tcgcgtcggc cccggtcgtc gggggagcgt gaggcgaggt cggcgaggtc gatcccggag
     1681 cagaagctac cgggcacgcc ggtgaccagc agcaccgtca cgtccgggtc ggcactcgcg
     1741 cgagcgacgt ccgcggagaa ctcgtcgaac atcgcgtacg tcatggcgcc gcgcttctcc
     1801 ggccggtcga tggtcagcgt ggctactcgg tcggtcacct catagcggat ctcgcgcacg
     1861 tgccgtctcc ctcttccccc gacgctggtg gcggtgcaat gctagctgaa cgatcgttaa
     1921 ggagggcctg tggatttcga actgcccgtc gagctgaccc agcgcctcgc cgacctcgat
     1981 gacttcatcg agcgggagat cagaccgctg caggcgcagg acgacaacga gcgtttcttc
     2041 gaccaccgtc gcgagtactc ccgcaccgac ttcgagaacg ggggcgtgcc gcggcgcgag
     2101 tgggaggagt tgctcggcga gatgttccgc cgcgccgacg ccgccggatg gctgcgctac
     2161 gggctgccga aggaggtggg cggctccgac ggaacgaacc tcgacatggc ggtgatccgc
     2221 gagcacctgg cacacaaggg ccttgggctg cacaacgacc tgcagaacga ggcgtcgatc
     2281 gtcgggaact tcccgttcgt ccacatgatg ctccagtacg gctcgcaggc gcagaaggag
     2341 gagttcctcg agccgatgct cacgcgcacg aagcgcatcg gcttcgggct gaccgaaccc
     2401 gagcacggca gcgacgcgac atggatggag accaccgcgg tccgcgacgg cgacgactgg
     2461 gtcctcaacg gcgcgaagcg cttcaactcg gggatgcact cggccaccca cgacgtgatc
     2521 ttcgcgagga catcggggaa gcccggtgac ccgcgcggga tcacggcgtt cttcaccccc
     2581 gtcgactctc ccggtttctc tgtcgacttc cactggtgga cgttcaacat gcccaccgac
     2641 cacgccgagg tcaccatccg cgatgtgcgc gtcccgagct ccgcaatctt cggggaggag
     2701 ggtgcggggc tggcggtggc ccagaccttc gtgcacgaga acaggatccg gcaggcggcc
     2761 tccggtgtcg gcgccgggca gtactgcgtc gaccgcagcg tcgagtacgc gcgtaagcgc
     2821 gtgacgttcg gccagccgct agcgacccgg caggcgatcc agtggccgct ggtggagctg
     2881 cagacggagg cagagatgct gcgcgggctg gtccgcaaga ccgcgtggga gctcgaccgg
     2941 gtgccgcaca tgcagatcag cgacaaggtc tcgatgtgca actaccgggc gaaccggttc
     3001 gtgtgcgagg ccgccgaccg cgcgatgcag gtgcacggcg ggctcggcta cacgcggcac
     3061 acgccgttcg aacacatcta ccgccaccac cgccgctacc ggatcaccga ggggtcggag
     3121 gaggtccaga tgcgcaaggt cgcgggctac ctgttcggct tcgccggacc gcggaaggga
     3181 cagggctgag acaggtggac gacttccgcg agctcggcgg ccggctgtcg aactggggcc
     3241 gctggggcgt cgacgggcgg cgtgacgagc ggggcacgac cgacctgctc accccggaga
     3301 ggatcgtcgc cgccgcgagc ctggtgcgcg acggcaaggt gttcgacctc ggcatcccct
     3361 tcggaccgga tggcccgcag cccggcggcg ggcgcatcaa ccccgtccaa ctgctctccg
     3421 agaccggcgc cgaccaggac ttccccggcg ccttccacta cgccgacgat tacgtcttca
     3481 tgccactgca gagcgcgtcg cagtgggacg gccttgagga actcatagcg cccgtcggcc
     3541 gcgcagccct cggcgagctt ctcgaagtcg aggatctcgc ccagcgtcat tcccatgtcc
     3601 cggcgccgcc catcaagttc cgccgggcgg tcggctcacc gatcaacccg ctggctatca
     3661 agtaagggga tctacgtgcg ggtagcgtgg tcgctccgcc ccaccgggac ggtgcagagg
     3721 aggtggccgc gtggcgaggc cagctctgcc ggagcccggc gtacgccgcg ccgggccgac
     3781 gccggcgcgc gtgccgcacg gcgcggtcgg cgaggcgggg atcctggacg cagcgctcgc
     3841 ggtcatggcg gagcacggct tccacggcac gtcggtccgg gacatcgcgg tgcgcgcggg
     3901 ggtcagcccg gcggcgctct accaccactt cgcgtccaag cagcaggtgc tcgcgacgat
     3961 catggagcgc gggatcaagg cgctcctgga gcgcacgcac gctgcgctgg cggacgcggg
     4021 cgacgagccg gcggaccggc tgcgggcgct cgtcgaggtg catgtgctgt tccacctcca
     4081 ggaccagcgg ggcacgctgc tcggcacgag cgagctgcgc gcgctggagg agccggtgcg
     4141 cagccgccac ctcgccaagc gccaccagca gcagcgcctg ttcgacgggg tgatcacgca
     4201 gggcgtggac ctcggcgtgt tcacgacgcc gatcccgctc gaggcctccc gggcgatcgt
     4261 cgtgatgtgc actggcgtgg cgaactggtt ctccccgaaa ggtcggatga gccgcgccga
     4321 ggtggtgcgc cgctaccagc ggctcgcact ggacatggtg ggggcgcacg acgcctgacc
     4381 cgctcggccc ggggtccgcg atgacggccg ccgcgacgct cgacgggcgc ccggtcgacg
     4441 cgggcgaccc ggatgtgctc gcccgcctgc tcgcgcgccg gctcacgacg gtgacgggtg
     4501 ccgaggtgtc cgtcgagcgg ctcacccgcc tcaccggtgg tgccagccgg gagacgtggt
     4561 cgttcgcggc gctcaccgat gacgggcacc gcaggctcgt gctgcgccgt gacccgcccg
     4621 gcccggggcg gccggagggg atggcccgcg aggccgccgc cgttgccgcc gcgcacgccg
     4681 cgaaggtgcc cgtgccgctg ctcgtcgact ccggtaccga cccggcggtg ctcggctcgc
     4741 cgtacctggt caccgagtac gtcgacggcg agacgatcgc gcgcaggctg ctgcgcgaac
     4801 cgcagtacgc gagtgcgcgc gagtcgatgg ccaccgagct gggccggacc ctggcccgga
     4861 tccacagcgt gccgctggtc gccgtgccgg gcctggagac ccacgaccca ctcgagcacc
     4921 tgctggccag ctacgacgcg ctgggcgagc cgctgccgac catcgagatc gcgctgcgct
     4981 ggctcgagcg gcgccggccg gacccggtgg cggagacggt ggtgcacggc gacttccgca
     5041 ccggcaacct gatcatcgct cccgacgggc tgcgcgccgt gctcgactgg gaggtggtgc
     5101 accgcggcga cccgcgggag gacctgggct ggctgtgcgt gaaatgctgg cgcttcggtt
     5161 cggccctgcc agctggcggg ttcggcaccg tcgaccagct gctcgacggc tacatggagg
     5221 tcgcgggcac gcggcccgat c
//
LOCUS       DASRSC010000201         5661 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5314340,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000201 DASRSC010000000
VERSION     DASRSC010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5314340"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VFE39_09960"
     CDS             <1..1048
                     /locus_tag="VFE39_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET6258963.1"
                     /translation="RLLTRTIGSAWADDIFSESAAAAFWQTLSLPPLLLGLFGVLGYV
                     GDWFGPDTVTAVQHRIIDLTSGVFSRNAVDEIIAPTVAQVLTTAHGEVVSLGFLLSFW
                     SGSSAMAAFVDAITRAYRQDRLRNIVWQRVLAILLYLVGLAIGIVFLPIVALSPDRLL
                     PLLPDAWEPAAAFVSGWLYYPLIGLVLVLALTTLYKIALPLKPPWYRGLPGALLAAVV
                     FLVGVTGLRVYLDWITGTGYTYGALAAPIAFLLGTFFIALAIVIGAHLNAAVQELWPA
                     PLRRRGRPVPAPGPERQAPADLADAIRHHPEAAAVVLERLEYVIARPVVTTAVATAGD
                     RQLNRRPPDAPDGS"
     gene            complement(1168..1806)
                     /locus_tag="VFE39_09965"
     CDS             complement(1168..1806)
                     /locus_tag="VFE39_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258964.1"
                     /translation="MRPAPGEQGPRGEQGASGEQGTPGEQGRPGERGEQGAPGEKGER
                     GEQGARGEQGLPGERGEKGEHGDKGLPGDQGPRGEQGLSGEKGAPGEQGPRGEQGARG
                     EQGLPGEQGPVGPAGPPGPQGAPGPQGLPGPQGQQGWAPAPSRQPQEAAARWVTPASA
                     KAVPGLTSGLELAFIPIQIFTSMLDTTLTVQKNVWNSVTAAASTGSRDAKRN"
     gene            2212..3204
                     /locus_tag="VFE39_09970"
     CDS             2212..3204
                     /locus_tag="VFE39_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6258965.1"
                     /translation="MSIVRWGVVGPGRIAAKVVDDFPHVPGAEVVAVASRSPQRAEAF
                     AGAHEIGRVHDSYRAIVEDDAVDVLYIATPHPHHRAVALAALKGGKAVLVEKAFTVTP
                     AATREIADAATDARVFAMEAMWTRFQPAVVRMRELIADGAIGEVRAVQGELGVHASTD
                     PLDRYYNIAIGGGALFDLTVYPISFAQMVLGTPDTVAAHGTLDATGVDIEEAVLLGWA
                     DGRTASLFTSLRCATPGQMRVFGTGGWIDLLPRFHHPDTFVLHRTGRDAEEVTVPHIG
                     GGYAHELREVTDCVLAGRTQSEVMPLADTIAVQDVMGAVADQLGMVPQEGPAEL"
     gene            3311..3457
                     /locus_tag="VFE39_09975"
     CDS             3311..3457
                     /locus_tag="VFE39_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6258966.1"
                     /translation="MQVRIGLVTVVVEEYDPAIAFFVDVLGFELVEDSPSLTPGWRAR
                     ASSS"
     gene            complement(3561..4826)
                     /locus_tag="VFE39_09980"
     CDS             complement(3561..4826)
                     /locus_tag="VFE39_09980"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6258967.1"
                     /translation="MTLLSERAIAPLDALRRQTFASLSNRNYRRYLTGQSISLVGTWM
                     QTIAQSWLVLQLTGSGTALGLVVALQTLPTLVLGPYGGVIADRMDKRRLMIGLQSVMG
                     VLALALGLLTITGAVQLWQVYVLAFVLGLNNCFENPARQSFVLEMVGSDDLRNAVSLN
                     SVLVNVARAVGPAVAGLVIATGGIGLCFLINAASFVAVVTSLLRLDVSALSPSNPTPR
                     APGQLREGLTYVRRTPRLAIPLLMMALVGCLAYEFQVVLPVVASQTFAGDARTYGFLT
                     AAMGAGAVVGGLYMAARGRTGMWSLVVGAAAFGLALLATALSPTLGVALVGMGLVGAA
                     SVAFQSAGNSTLQLAAAPHMRGRVMALWAVAFLGSTPIGGPIAGLVSQHFGGRAGLAL
                     GAVACIVAAGLGAVVARRESAARSAEVIG"
     gene            complement(4823..5281)
                     /locus_tag="VFE39_09985"
     CDS             complement(4823..5281)
                     /locus_tag="VFE39_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6258968.1"
                     /translation="MDSFDGEDVTRLRVALARISRLLDRQSRGNSLTSTQTSVLATVA
                     RLGPIRLSELADVEGINPTMLSRIVGKLEDGGLLRRRPDPDDGRAARVECTDAGSAEH
                     LRLRAERTQLLTARLAAMPAARAAELLAALPALEALADQLTLATTRKENR"
BASE COUNT          777 a         2068 c         1952 g          864 t
ORIGIN      
        1 ccgactcctg acccgcacca tcggtagtgc gtgggccgac gacatcttca gcgagtcggc
       61 cgcggcggca ttctggcaga cgctgtcgct gccgccgctg ctgctcggac tctttggtgt
      121 gctcggctac gtgggcgact ggttcggccc cgacacggtc acggccgtcc agcaccggat
      181 catcgacctc accagcggcg tgttcagccg caacgccgtc gacgagatca tcgccccgac
      241 ggttgcccaa gtcctcacga ccgcccacgg cgaggtcgtc tcgctcgggt tcctcctcag
      301 cttctggtcg ggttcgtcgg cgatggccgc cttcgtcgac gcgatcacgc gggcctaccg
      361 tcaggaccgg ctacgcaaca tagtctggca gcgggttctc gccatcctgc tgtacctggt
      421 cggcctggcc atcgggatcg tgttcctgcc gatcgtcgca ctgagtcccg accgcctgct
      481 tcccctgctt cccgatgcgt gggagcccgc tgcggcgttc gtgtccgggt ggctgtacta
      541 cccgctgatc gggctggtgc tcgtgctggc gctcaccacg ctctacaaga tcgcgctacc
      601 gctcaagccg ccgtggtatc ggggcctgcc cggtgcgctg ctggccgccg tcgtgtttct
      661 cgtcggtgtg acgggcctgc gcgtctacct cgactggatc accggcaccg gctacaccta
      721 cggtgcgctg gcagcgccca tcgcgttcct gctcggcacc ttcttcatcg ccctcgcgat
      781 cgtcatcggt gcgcacctca acgcagcggt gcaggagctg tggcccgccc cgttgcggcg
      841 acggggcaga ccagtaccgg cgcccggccc ggagcgacag gctccggcgg acctcgccga
      901 tgcgatccgc caccatcccg aggccgccgc tgtggtcctg gaacgcctcg agtacgtcat
      961 cgcgcgccct gtggtcacca cggcggtcgc gacggcaggc gaccgacagc tcaaccgccg
     1021 gccgcccgac gctcccgacg gttcctgaac aggccgaccg gcaggtcgag cgcgatcacg
     1081 tgcgcgtgcg gctcccggca gagcgcagcc ggcggacgtc ctctccgtcg tagcggtgag
     1141 aacgtccgcc ggccgacgat ggagcgatca gttccgcttc gcgtccctgg agccggtgct
     1201 cgcggcagcg gtcaccgagt tccacacgtt cttctgcacc gtcagcgtcg tgtcgagcat
     1261 ggaggtgaag atctggatcg ggatgaacgc cagttcgagc ccggacgtga ggccgggcac
     1321 cgctttcgcc gatgccgggg tgacccaacg cgccgctgcc tcctgcggct ggcggctggg
     1381 cgccggcgcc caaccctgct gcccctgggg gccgggaagt ccttgtggtc cgggagctcc
     1441 ctgaggcccg ggcggcccag ccggtcccac cgggccctgc tcgccgggca ggccctgctc
     1501 cccgcgtgca ccctgctcgc cgcggggtcc ctgttcgccg ggcgcgccct tctcgccgga
     1561 cagaccctgc tcaccgcggg gcccctggtc gccgggcagg cccttgtcgc cgtgctcgcc
     1621 cttctctccg cgctcgccgg gcaggccctg ctccccgcgt gcgccctgct cgccacgctc
     1681 gcccttctca ccaggcgcgc cctgctcgcc gcgctcgccg ggcctgccct gctcaccagg
     1741 ggtgccctgc tccccgcttg caccctgctc gccgcggggg ccctgctcgc cgggcgcggg
     1801 gcgcacgccc tgcgcgctac gcggccgtgc ctctcccgct ctgccggggc cagccggctg
     1861 accggtcgtg cccggctccc gacgcgacga ctgcccggcc cgaggacgat ccccattgcc
     1921 cacgggctgc tccgcgccgg tgctccggcg gaccgtcgag ctcggctcgc gtgcttcctc
     1981 tgccatatgc gtctccttcg tcgtccatcc gtacgtgcta tgcctgcgtg tgccgctgcc
     2041 gggtcccggc gctcactgct gctaggtatc ccaggcgctg atcagtaacc cacgcgggtg
     2101 ggtggggctc gtcacaatcg ttctcgaacg tccgttcgag ttgtccacgg gcctgtccac
     2161 acctgtggac gcgctcggca tccaggaacc ggcccggcca ggatgggtgg catgagcatc
     2221 gtccgctggg gagtggtggg tccgggccgg atcgcggcga aggtggtgga cgacttcccg
     2281 cacgtgcccg gagcggaggt cgtcgcggtc gcgtcccggt cgccgcagcg cgccgaggcc
     2341 ttcgccggcg cccacgaaat cggccgcgtc cacgactcct accgggcgat cgtcgaggac
     2401 gacgccgtcg acgtgctgta tatcgccacc ccgcacccac accaccgggc cgtcgctctc
     2461 gcggcgctca agggcgggaa ggccgtgctg gtggagaagg cgttcacggt gacgcccgcc
     2521 gccacccggg agatcgccga cgcggccacc gacgcacggg tcttcgcgat ggaggccatg
     2581 tggacccgct tccagcccgc cgtcgttcgg atgcgggagc tgatcgccga cggcgcgatc
     2641 ggcgaggtcc gcgccgtcca gggggagctc ggggtgcacg cctcgaccga cccgctggac
     2701 cggtactaca acatcgcgat cggcggcggc gccctgttcg acctcaccgt gtacccgatc
     2761 tcgttcgccc agatggtgct cggcacaccg gacacggtcg ccgcccacgg cacgctcgac
     2821 gccaccggag tcgacatcga ggaggccgtg ctgctcggct gggccgacgg ccgcacagcc
     2881 tccctgttca cctcgctccg ctgtgccacc cccgggcaga tgcgcgtgtt cggcaccggc
     2941 ggctggattg acctcctgcc gcgtttccac catcccgaca cgttcgtgct ccaccgcacc
     3001 ggccgcgacg cggaggaggt gacggtgccg cacatcggtg gcggctacgc ccacgagctg
     3061 cgtgaggtca ccgactgcgt cctcgccggg cggacgcaga gcgaggtcat gccactcgcc
     3121 gacaccatcg cggtgcagga cgtgatgggc gcggtagccg accagctcgg catggtgccg
     3181 caggaagggc ctgccgagct ctgaccgcga ggctggggcg ggaccggacg ctccgagcgc
     3241 tctccgtggt cctcgctgcc ccgtggccct gggcgcgtca ggctcagcgt ggagggttca
     3301 tcatcgccgg gtgcaggtgc ggatcggtct ggtcaccgtc gtcgtcgagg agtacgaccc
     3361 cgcgatcgcc ttcttcgtcg acgtcctcgg tttcgagctc gtggaggact cgccgtcgct
     3421 cacgcccgga tggcgcgcgc gggcgtcgag ttcatgaccc cgacccggtc ggagcgctac
     3481 ggccgcgtcg ccgtgttcct cgacatcgcc gggaaccggt gggacctgct cagaccgccc
     3541 tgaacaccgc ccccaaggtc tcacccgatg acctcggcac tcctggcggc tgattcccgc
     3601 cgggcgacga ccgcacccag accagccgcg acgatacagg ccaccgcacc gagtgccagc
     3661 ccggcgcgtc ccccgaaatg ctggctgacc agcccggcga tcggaccgcc gatcggcgtc
     3721 gacccgagga acgcgacggc ccacagcgcc atcacgcgcc cacgcatgtg cggggcggcg
     3781 gccaactgca gcgtgctgtt gcccgccgac tggaacgcga cgctcgccgc gccgaccagg
     3841 cccattccca ccagtgccac cccgagagtg ggcgagagtg cagtggccag cagcgccagg
     3901 ccgaacgcgg cggctccgac gaccaatgac cacatcccgg tgcgtccccg tgcggccatg
     3961 tagaggccgc cgaccacggc gcccgcaccc atcgccgctg tcagaaaccc gtaggtccgc
     4021 gcgtcaccag caaacgtctg gctcgcgacg accggcagca ccacctggaa ctcgtacgcc
     4081 aggcacccga ccagcgccat catcagcagc gggatcgcga gcctcggcgt ccggcggacg
     4141 taggtcaggc cctcgcgcag ttgcccagga gcgcgcggcg tcgggttcga gggggagagc
     4201 gccgagacgt cgagccggag gagcgacgtg acgaccgcga cgaagctcgc cgcgttgatc
     4261 aggaagcaca agccgatgcc cccggtggcg atgacgaggc cggccacggc cgggccgacc
     4321 gcgcgggcaa cgttgacgag caccgagttg aggctcaccg cgttgcgcag atcgtcggat
     4381 ccgaccatct ccaggacgaa cgactggcgc gccggattct cgaagcagtt gttcaggccg
     4441 agcacgaacg cgagcacgta tacctgccac agctgcacag cgccggtgat cgtcaacagc
     4501 ccgagcgcca gggcgagcac gcccatcacc gactggaggc cgatcatcag ccggcgcttg
     4561 tccatccgat cggcaatgac gcctccgtag ggaccgagca cgagcgtcgg cagcgtctgc
     4621 agcgcgacga cgagaccgag tgcggtaccg gagccggtga gctgcaggac cagccaggac
     4681 tgggcgatcg tctgcatcca ggtgccgacg agcgagatcg actgaccggt caggtagcgg
     4741 cggtagttgc ggttggagag ggaggcgaag gtctgtcggc gcagcgcgtc gagcggggcg
     4801 atcgctcgct cgctgagcaa tgtcatctgt tctccttgcg ggtggtggcg agggtgagct
     4861 ggtcggcgag ggcttcgagg gccggcagcg cggcgagcag ctcggccgcg cgggctgcgg
     4921 gcatcgccgc gaggcgggcc gtgagcagct gggtgcgttc ggctcgtagt cgcaggtgct
     4981 ccgcgctgcc cgcatccgtg cactcgacgc gcgcagcgcg cccgtcatcg ggatcggggc
     5041 gccgccgcag caggccgccg tcctcgagtt tgccgacgat gcgcgagagc atcgtcgggt
     5101 tgatgccctc gacatcagcg agctcggaca agcgtatcgg cccgaggcgc gcaaccgtgg
     5161 ccaggacgga tgtctgtgtg ctggtcagcg agttgccgcg ggactgccga tcgagcaggc
     5221 gcgagatgcg ggcgagtgcg acgcgtagcc gggtgacgtc ctcgccgtcg aagctgtcca
     5281 tgtcgccctc ctcatataat tgcgtgccga caagcaagta tagcaccctg cgtgttagga
     5341 gtgctggtcg cggcaggtgc gttggtcaca gacgacgcag gcgtcgagca gctcaccgtg
     5401 gtgcccttgt cgttggcgag ctcggggtgg cgggcggtga agcgccgtcc atcgtggcat
     5461 cgtcgtcgcc ggggagccgt tgatcgaccg ccggtcggcg gcgcaaccca gcggcccgcg
     5521 aaagcaccgc cggaacgcat ccgccgcagt agagtccgcg gacgtggacc gcgcagagat
     5581 cacgtccctg gccgagctcg tggacgtcgt cggaactccg attccgcgtg tcgcgcagaa
     5641 ggaacgccag gttctcgacc c
//
LOCUS       DASRSC010000202        18759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_403083,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000202 DASRSC010000000
VERSION     DASRSC010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 18759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18759
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403083"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VFE39_09990"
     CDS             <1..923
                     /locus_tag="VFE39_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020632924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HET6258969.1"
                     /translation="GLGEVVIDVVASAVNRADLMQRQGHYPPPPGASDIIGLECSGRI
                     AQLGDGVEGWQVGDEVCALLAGGGYAEKVVVPAAQLLPIPSGVDIVTAAGLPEVACTV
                     WSNVVDAGRLSAGETFLVQGGSSGIGTHAIQVAKALGARVAATAGAPDRLERCRELGA
                     DIVIDYHDDIPAELKKATDGHGADVILDNMGAKGLAANLDALAPDGRLLIIGLQGGAK
                     GELNLNKLLVKRASVTAMGLRGRPIDGPHGKGAVVAGVREQVWPLIAEGKVQPIVHAT
                     FPMPDVAEAHAQLEAGGVIGKVLLVTPAKS"
     gene            1065..1625
                     /locus_tag="VFE39_09995"
     CDS             1065..1625
                     /locus_tag="VFE39_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial proteasome activator family protein"
                     /protein_id="HET6258970.1"
                     /translation="MSQPSEQHTNDKGEQQVMVIGPDGRPVGMATVPQSGEDGGDAQG
                     PGAGIEQPGKVMRIGTMIKQLLEEVRAAPLDDASRNRLREIHENSIKELEGGLAPELR
                     DELHRLTLPFSETTIPSDAELRIAQAQLVGWLEGLFHGIQTALFAQQMAARAQLEQMR
                     RGLPPGTMPQQPGAEAGLGRGTGQYL"
     gene            1749..2636
                     /locus_tag="VFE39_10000"
     CDS             1749..2636
                     /locus_tag="VFE39_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6258971.1"
                     /translation="MARTMTTGDDRAEDTTSAPGVVPDPPGSRSWQRAFDDLGRGWRQ
                     RPLWGYLGWQDIKQRYRRSVLGPLWISITMAVVATAMGILYGALFGQDLRTFLPYVAT
                     GLLIWYFINGCILEGSEVFIANEGLIRFLPAPLSLHVYRLVWRQTLFFLHNLVVWGIL
                     AAIFRPPLGWSLLLAVPAFMLLVINGAWISVVTGIIATRFRDIPPIIASLANLLFFVT
                     PIVWQYDTLLKNPAVAERARIAELNPVLHFVEILREPLLGEPIVWRHWAVVGVITVAG
                     CLAALVCLRNYRSRVAYWV"
     gene            2644..3411
                     /locus_tag="VFE39_10005"
     CDS             2644..3411
                     /locus_tag="VFE39_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6258972.1"
                     /translation="MVSIDLDGAAVDFPIFDAKTRSLKKAVLGRAGGRIGTDSKVPII
                     EALRDITVSLRKGDRVALVGHNGAGKSTLLRLMSGIYEPTRGRSSVIGKVAPVFDLAV
                     GMDPEISGLENILIRGLFLGMTRKQMEARVDDIAAFTELGDYLKMPLRTYSTGMRVRL
                     ALGVVTSIDPEILLLDEGIGAVDAEFLAKARTRLNELVERSGILVFASHSDEFLADLC
                     DTAIWMDRGTIREHGPLRDVLRHYKGRDVLAEIEAAR"
     gene            3408..4334
                     /locus_tag="VFE39_10010"
     CDS             3408..4334
                     /locus_tag="VFE39_10010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005460971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6258973.1"
                     /translation="MRHEPLPAGSVVAVVVTRHRADMLVDSLAALAKQTHPIAHLVVV
                     DNGPDQPAREVVEASGLPATWLPSWNNLGGAGGFALGMLHALAMGAEWIWLGDDDGYA
                     ADETVLATLVDLADRRGLAAVSPTVADKDEPDRLAFPIRRGLTWHRSRAALAADVDHG
                     ADDELLPGVAALFNGALFRAATLDVVGVPDLRLFVRGDEVEMHRRLVRSNLPFGTSLR
                     ATYLHPAASDDDKPMLCGRMHARDPDDPVKRYYTYRNRGYLVSQPGMRKVGLLEVPRF
                     AWYFLVTKRSPRQFAQWVRLVRQGRAERFHRA"
     gene            4624..5862
                     /locus_tag="VFE39_10015"
     CDS             4624..5862
                     /locus_tag="VFE39_10015"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6258974.1"
                     /translation="MWDWLRFLEHWQLFLPVGVIGGISWSVWLIRKLMSARYRPLTND
                     FRTTTSVVVPSFREDPNVLMRCLDTWLAQGPTEVIIVLDIEDLEARDRLMARQDRRLH
                     VMMFAHEGKRSALGVGIRAARHEILVLTDSDTAWEPGLLGAVQMPFVNSKVGAVGTRQ
                     NVHEPRSSVWRRVADWIIDLRYMDYVPATARRGGVVCVSGRTAAYRRSAVMPVLANLE
                     HEYFLGRRCVAGDDGRLTWLVLAQGYRTEHQDSARALSMFPSTFRAFCKQRVRWSRKS
                     YRCYLTAIYKGWLWRAPLISQLTVFQILFTPVTMFATVWYLVAAWFSPQRELAVILGL
                     AWLFIGRAIRSFSHLRRRPSDVVILPLVTLVIMFVALPIKAWAFVTMNKQGWLTRRAD
                     LVGGEGQDEASLTEDSRGAG"
     gene            5849..7762
                     /locus_tag="VFE39_10020"
     CDS             5849..7762
                     /locus_tag="VFE39_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET6258975.1"
                     /translation="MAQADPPRRSAGRRWLVLLTALAVVGGMGVAAVQTGLVDRVVTG
                     LGPVDAGSRLDDAEEVGAVVPPTDAEDAAPTTEAQQRQATLVEAEDDRLFAIVSDRPP
                     SDVPYEVAAAGQAVPTVVLTARSNPYDLPALERLGAAARLPDGSWLLSRSVVVARGAE
                     LRIQEPGATLRLASGPSGFATLVAFKGTLTLSGGPDAPLTLTSWDPATNAADAERGDG
                     RAYIRAVGARMDLTAVHVSDLGFWSGRTGGVAWTGSAGAAATGSANHTTIERGHYGIF
                     TSRTKDAVVNDTILRDNELDGLLLHRETSGLVATGVTSTGNGRDGITATRGAQRITLS
                     QVTASDNAGSGIRIDGSPLAAEATAGGASTAPGRGYTIERSTAAGNTEVGILAVEAAG
                     LVLRNNSVSGNRDGIVVRGGTDRPTVSGNAVDAAEFGIAVRSGARDAQVSGNRIGSAV
                     IAIQVTDASAKIHQNEVRAASRYGVSLVGEVTGSSVAANKLAGRGLSALDVNRVGTTA
                     TVAISGNDEARWNVDRDELRYWRDYAVDHPLLLLWLLILLLPLAARVWTKRRRRIRDE
                     HAHPYVNVPPGPPVPGNSNVITSIGRPAPTAPRPPPPVVPPAATPPPQDVTATVALPV
                     TRVTVVSGRGARR"
     gene            7759..8835
                     /locus_tag="VFE39_10025"
     CDS             7759..8835
                     /locus_tag="VFE39_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HET6258976.1"
                     /translation="MTPPPDEVTRGRFHRRVNRPRVTSILGGLVAVGVLAGVAACGAG
                     GGTGPPPPPTTAPLPAAGTACTQNPTTEVHNADELTEALAGARTGSVIRMAAGTYRGM
                     FVATAQGTPDKPIALCGGRDAILDGGTTDGGYAFHLDGATHWELSGFTVRGGQKGVMA
                     DRTQGTVIDGLLVEGTGDEAIHLRQNSTDNVVRGNTVQRTGLRNGKFGEGIYIGSAES
                     NWCEQTNCEPDRSDRNVIEGNTISGTTSESVDIKEGTTGGILRGNTFSGAGMTDADSW
                     VDIKGNGWSIVGNVGTDSPEDGFQVHEILDGWGLDNTFDSNTATVNADGFAINVTKNK
                     ERNRVGCDNRGIDAGQGLSPEACG"
     gene            8884..10188
                     /locus_tag="VFE39_10030"
     CDS             8884..10188
                     /locus_tag="VFE39_10030"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HET6258977.1"
                     /translation="MFARRIAVIGTGYVGLTTGACLASLGHHVVCADIDRDKVDRLND
                     GQIDILEPGLPELVSEGVAAGRLEFVLGARQAIVRPDEPAVEVVFLCVPTPMGEGGAA
                     DLAAVQAVANEVRHLLPKDCVVVNKSTVPVGTAEATRKMLGRRDVSVVSNPEFLREGS
                     AVHDFLHPDRIVVGSDAQDAAERVAALYSKLGAPTQLTDAASAEMVKYAANCFLAMKL
                     SYVNALADLCERLGADVIEVTEGMGYDRRIGQTFLSPGPGWGGSCFPKDTHALLQIAA
                     AAGMDFELVGASLRTNETTRLRVVEKVRAAVGGSLAGQRIGLLGLTFKAGTDDLRDSP
                     ALAVAALLRAEGAELTGYDPACPQDVPGVTDGIIVVDDALDAAKDAVALVVLTEWQQF
                     RSLDWTALGGVVGAPVVIDTRNLLDPDVLRRAGFAWIGLGRS"
     gene            complement(10245..10658)
                     /locus_tag="VFE39_10035"
     CDS             complement(10245..10658)
                     /locus_tag="VFE39_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET6258978.1"
                     /translation="MTATRPAPAQLGLVAQLSRFVAIGVLSALVDFGSYQGLLALGTW
                     VHAAKAISFILGTTTAYLLNRRFTFTVDGAGRARFAGFVLLYGTTFALNIGVNALMLA
                     VLPAIPMRVSLAWVIAQGAATAINFVMLRTVVFRR"
     gene            10769..12463
                     /locus_tag="VFE39_10040"
     CDS             10769..12463
                     /locus_tag="VFE39_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HET6258979.1"
                     /translation="MTTVTDADTPTGVRALADDYVRRLADLDTRVATMLGTHPGDDRV
                     PNLSPDGQSATDELARRVLTALPTAVVADEDDRRCARLLRERLEAQLAVSETGEHLRA
                     VRNIVGPHQQVRQLFELMPTATSDDWAVVARRMAKVPQAYSGYVASLTEGSRRGLHAA
                     PRQVETVIGQLDAWLTDAGGSWFQAFVAKAADVTGPTVRDALHEGAGAATAAAAELRD
                     FLAREYLPAAQGTPDGVGAERYRIGARVSTGSDLDLAEAYAWGWDEFRRLDAEMRREA
                     EGVLAGAGPVMAMRHLDENGAAVDGVDEVRAWLQQMMDTAMDELDGVHFDIAAPVRTV
                     EAMIAPSGSAAAPYYTRPSLDFSRPGRTWLPTLGQHRFPLWGLISTWYHEGVPGHHLQ
                     LAQWTHLANRLSTYQTSVGSVSGNTEGWALYSERLMDELGYLTPPGARLGYLDAQQMR
                     AIRVVIDIGMHLGLQVPADSPLLAGETWTPEVARQFFGAHSGRPGPFLDSEIVRYLGW
                     PGQAISYKLGERAWLAGRDAAMTARGADFDLKAWHAAALSLGSLGLDDLTAELARL"
     gene            complement(12479..13081)
                     /locus_tag="VFE39_10045"
     CDS             complement(12479..13081)
                     /locus_tag="VFE39_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HET6258980.1"
                     /translation="MSTTPPDPPGIRISDADRERAAAQLHKALAEGRITVSELEERLS
                     VVYAARYEADLRPPLADLPGAHQLAVPASAPQSAPDAPPLVLRTGASTIKRRGAWDVP
                     PRLRLQSAMGSVVLDFCDTALPYDVIDIEVDLGAGSARLLVPDESTANIDRVVASMGT
                     VKSSVPATRRPGVPHFVVHGRAGMGSVSVRRRYHFAGHYF"
     gene            13333..14694
                     /locus_tag="VFE39_10050"
     CDS             13333..14694
                     /locus_tag="VFE39_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6258981.1"
                     /translation="MSGTASLRGWGRTAPTTAQVHEPRSVDDVRAAVAAAGPRGIIAR
                     GLGRSYGDPAQNAGGTVLDMTGLATIHSIDADSGIAVVDGGVSLDTLMRRALPYGLWV
                     PVLPGTRQVTIGGAVGADVHGKNHHSKGSFGNHVLSLDLVTADGALRTLTPDGPDAEL
                     FWATVGGMGLTGVIVRATVQLHRTESAYFVVDTDRTADLDELMGLLTDGSDDTYGYSA
                     AWFDTTTTGAQLGRAVLTRGSLATLDQLPPKLRHDPLKFEAPQLLSFPDVFPNGLANR
                     ITLRAFSELWYRKAPKRQRGSVQNITTFYQVLDLFGDWNRVYGSRGFLQYQFLVPFGE
                     EPTFRKVVEKIAQSPHASGLNVLKRFGEGNAAPLSFPQPGWTITVDFPIARGLHRFCE
                     ELDELVLGAGGRLYLAKESRASAATIRRGYPRFEEWRKVRDAADPTGVFTSDMARRLE
                     LTN"
     gene            14694..15449
                     /locus_tag="VFE39_10055"
     CDS             14694..15449
                     /locus_tag="VFE39_10055"
                     /EC_number="1.1.1.333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenylphospho-beta-D-erythro-pentofuranosid-
                     2-ulose 2-reductase"
                     /protein_id="HET6258982.1"
                     /translation="MIDAVGNPQSVLLMGGTSEIGLAVVEAFAADRPLRVVLAARPSE
                     RLDAARARLEGLGCAVETLPFDARALDTHADVVRKAFTGGDIDVAVVAFGLLGDNEQA
                     WTDVETAVELAQVNYTAAVSVGVALAERLRAQGHGALVAFSSVAGERARRSNFVYGST
                     KAGMDAFYSGLTEALRPSGVGVTVVRPGFVHTRMTEGRKPAPLSTTPEAVAAVVVDAV
                     RNRRELVWAPAPLRVLMSVLRHLPRAVFRRLPV"
     gene            15570..15935
                     /locus_tag="VFE39_10060"
     CDS             15570..15935
                     /locus_tag="VFE39_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6258983.1"
                     /translation="MKYMLIMQLNPVAWDALSPEQRDEVMNGHGAFLDTIKASGEMVE
                     THALAEPAQSAVVRVRGGVPAVTDGPFLEAKEFMGGFYIVECETRERALELAGLIPDA
                     GVEGMGIEVRPVMFSAGPE"
     gene            complement(15974..16765)
                     /gene="trpC"
                     /locus_tag="VFE39_10065"
     CDS             complement(15974..16765)
                     /gene="trpC"
                     /locus_tag="VFE39_10065"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET6258984.1"
                     /translation="MSVLDGILDGVREDLEQRQNDVSLAELKARAAAAPPPLDPLPAF
                     RTTGVSIIAEVKRKSPSKGALADIPDPARLAGEYAAGGAAAISVLTEQRRFGGSLADL
                     DAVRKSVDVPVLRKDFMVTQYQLWEARAHGADLALLMVVSLGGGLLADMLGLSRELGL
                     TALVEAHTAAELERAIEVGADVIGINARDLTTLKVDRSVFAELAPHIPEDAVRVAESG
                     VAGPEDVAAYRRSGADVVLVGEALVRAGDPRSSVGHFIAAANAQV"
     gene            complement(16867..18384)
                     /locus_tag="VFE39_10070"
     CDS             complement(16867..18384)
                     /locus_tag="VFE39_10070"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET6258985.1"
                     /translation="MTRAGLEPTTPSGSRGALHGELRNLTGLRIVAAAWVVVFHFHFT
                     PLPGVTDVVDVLGPLITSGALGVDLFFVLSGFVITYTYLDALGPALRARATARFVWAR
                     ACRVWPAYAVVFHLFGLWLVARAVFGSDSNIAFQAVQPELGPGEYVQQLFMVQLWDNP
                     FFDGASWVGSTWSISAEWLAYLLFPLAVLVLFRVRNLPAVVLGVAALALMAPMAWAYL
                     SSGSPYYPWSWLVRILCGFSGGALMYLAVRKMRRTARMQLGASVLAVVLPVLIAAGLL
                     LGELVGPGRGGAVIALFPLLVGALAVADRGPAMVLSAEWAVYGGRISYCLYLVHIPIF
                     ELYWLALQKFSWLGPDTPLAHVIGALVLMSTLTVAALAYRVVEEPARRRMRRMVSVGG
                     PSTPMVTPPVPPRHRAVGGAGTGWPEVGNTSAVSRTAARRRALATGNSRESGTGDGAA
                     PFARLSSPAPRHAASGTRQPTLAAALIDAQRDRPAHRGDYERAAYVRGGFLNVES"
     gene            complement(18544..>18759)
                     /locus_tag="VFE39_10075"
     CDS             complement(18544..>18759)
                     /locus_tag="VFE39_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HET6258986.1"
                     /translation="AWSFQIAARARGLGSAWTTLHLRYEKEIAELLGIPPNVRQGVLL
                     PTAYYTGETFKPAPREPLDTVLHLDRW"
BASE COUNT         2859 a         6604 c         6597 g         2699 t
ORIGIN      
        1 cgggcctcgg agaggtcgtc atcgacgtgg tggcgagcgc ggtcaaccgc gccgacctga
       61 tgcagcgtca gggccactac ccgccgcccc ctggggcgtc ggacatcatc gggctggagt
      121 gctccggccg gatcgcgcag ctcggcgacg gcgtcgaggg gtggcaggtc ggcgacgagg
      181 tgtgcgccct gctcgccggc ggcgggtacg cggagaaggt ggtggtgccc gcggcgcagc
      241 tgctgccgat cccgtccggc gtggacatcg tgacggccgc tgggctgccc gaggtggcgt
      301 gcacggtgtg gtcgaacgtc gtggacgcag ggcggctcag cgccggcgag accttcctcg
      361 tccagggcgg gagcagcgga atcgggacgc acgcgatcca ggtcgcgaag gcactcgggg
      421 ccagggtcgc cgccacggcg ggtgccccgg atcggctgga acgctgccgc gagctcggcg
      481 ccgacatcgt catcgactac cacgacgaca tcccggccga gctgaagaag gccaccgacg
      541 gccacggcgc cgacgtcatc ctcgacaaca tgggcgccaa gggcctcgcc gccaacctcg
      601 acgccctcgc ccccgacggc cggctgctga tcatcgggct gcagggcggc gctaagggcg
      661 agctgaacct caacaagctg ctggtcaagc gggccagcgt cacggcgatg gggctgcgcg
      721 ggcggccgat cgacgggccc catggcaagg gcgcggtggt cgcgggcgtc cgggagcagg
      781 tgtggccgct gatcgcggag gggaaggtgc agccgatcgt gcatgcgacc ttcccgatgc
      841 ccgacgtcgc ggaggcacac gcgcagctcg aggcaggtgg cgtgatcggg aaagtgctgc
      901 tggtgacgcc ggccaagagc tgaggacgga gcgactgcca ccgggtctca aggccctcgt
      961 ggctgggtat gtcccgttct ccgtgcggat acggtagcga gaagaacacg tcgaacagtg
     1021 atgcggcgag ggagaaccgt gctcgccggg aaacatgggg acccatgagc cagccatcgg
     1081 agcagcacac gaacgacaag ggcgagcagc aggtcatggt catcgggccg gacggcaggc
     1141 cggtgggtat ggcaacggtg ccgcagtcgg gcgaggacgg cggcgacgcg cagggcccgg
     1201 gggccgggat cgagcagccg ggcaaggtca tgcggatcgg cacgatgatc aagcagctgc
     1261 tggaggaggt acgcgccgcg ccgctcgacg acgcgtcccg caaccggctc agggagatcc
     1321 acgagaactc gatcaaggag ctggagggcg gtctcgcacc ggagctgcgc gacgagctgc
     1381 accgcctgac cctgccgttc tccgagacca ccatcccgtc cgacgcggag ctgcggatcg
     1441 cgcaggcaca gctcgtcggg tggctggagg gcctgttcca tgggatccag acggcgctgt
     1501 tcgcgcagca gatggccgcc cgcgcccagc tggagcagat gcgcaggggg ctgccccccg
     1561 gcaccatgcc acagcagccc ggcgccgagg ccggcctggg tcggggcaca ggtcagtacc
     1621 tgtaacccgg ccgccgctca ccgttgcagg ggagcaacgt tcgtgcgacg gcatcgcgtg
     1681 gacgttgctc tgctgcattc ggggcggggc cgcacgagcg cctcatcacc cgccggtact
     1741 ctcgtcgggt ggctcgaacg atgaccaccg gtgacgaccg ggcagaggac acgacgtccg
     1801 cgccgggggt cgtgccagac ccacccgggt cgcgcagttg gcagcgggca ttcgacgacc
     1861 tcgggcgggg ttggcggcag cggccgctgt gggggtacct cggctggcag gacatcaagc
     1921 agcgctaccg ccgctcggtg ctcggaccgc tgtggatcag catcaccatg gccgtggtcg
     1981 ccacggcgat gggcatcctc tacggggccc tgttcggcca ggacctccgg acgttcctgc
     2041 cctacgtggc caccggcctg ttgatctggt acttcatcaa cggctgcatc ctcgagggca
     2101 gcgaggtctt catcgccaac gagggactga tcaggttcct cccggcgccg ctgagcctgc
     2161 acgtctaccg gctggtgtgg cggcagacat tgttcttcct gcacaacctc gtggtgtggg
     2221 ggatcctcgc cgccatcttc cggccgccgc tcggctggtc tctgctgctg gcggttcccg
     2281 ccttcatgct gctcgtcatc aacggggcgt ggatctccgt ggtcacgggc atcatcgcca
     2341 cccggttccg cgacatccct ccgatcatcg cgagcctcgc gaacctgctg ttctttgtga
     2401 cgccgatcgt gtggcaatac gacacgctgc tgaagaatcc ggccgtggcc gagcgggccc
     2461 ggatcgcgga gctgaacccc gtgctgcact tcgtcgagat cctccgcgag ccgctgctgg
     2521 gcgagccgat cgtctggcgc cactgggccg tcgtcggcgt catcacggtg gcgggctgcc
     2581 tcgcggcgct cgtctgcttg cgcaactacc gctcccgcgt cgcgtactgg gtctgaggac
     2641 ggcatggtca gcatcgatct cgacggcgcc gccgtcgact tcccgatctt cgacgccaag
     2701 acgcggtcat tgaagaaggc cgtgctcggc cgcgcgggcg gccggatcgg caccgactcc
     2761 aaggtgccca tcatcgaggc gctgcgcgac atcacggtgt ccctgcgcaa gggcgaccgc
     2821 gtcgcgctcg tcgggcacaa cggggcaggc aagtccaccc tgctgcggct catgtcgggc
     2881 atctacgaac ccacccgcgg gcgctcctcc gtgatcggca aggttgcacc ggtcttcgac
     2941 ctggccgtcg ggatggaccc ggagatctcc gggctggaga acatcctcat ccgcggcctg
     3001 ttcctcggta tgacgcgcaa gcagatggag gcgcgggtcg acgacatcgc ggccttcacc
     3061 gagctcggcg actacctcaa gatgccgctg cgcacctact ccacgggcat gcgcgtgcga
     3121 ctcgcgctcg gcgtcgtcac cagcatcgac cccgagatcc tcctgctcga cgagggtatc
     3181 ggcgcggtcg acgccgagtt tctcgccaag gcgcgcaccc ggctcaacga actggtggag
     3241 cgctccggga tcctggtgtt cgcgtcgcac tccgacgagt tcctcgcgga cctgtgcgac
     3301 acagccatct ggatggaccg gggcaccatc cgggagcacg gtcccctccg tgacgtgctg
     3361 cgccactaca agggccgcga cgtactggcc gagatcgaag ccgcccggtg agacacgagc
     3421 cgctccccgc aggctcggtc gtcgcggtgg tcgtcacgcg ccaccgcgcc gacatgctgg
     3481 tcgactcgct ggccgcgctc gcgaaacaga cccaccccat cgcccacctc gtcgttgtcg
     3541 acaacgggcc ggaccagccc gcgcgggagg tcgtcgaggc cagtggcctc ccggccacgt
     3601 ggttgccctc gtggaacaac ctcggcggag ccggcgggtt cgccctcggc atgctgcacg
     3661 cgctcgccat gggcgcggag tggatctggc tgggcgacga cgacggctac gcggccgacg
     3721 agacggtgct cgccaccctc gtcgatctcg cggatcgccg tgggctggcc gccgtgtcgc
     3781 ccaccgtggc cgacaaggac gagccagacc gccttgcgtt cccgatacgt cgggggctca
     3841 cctggcaccg gtcgcgggcg gcgctcgccg ctgacgtcga ccatggcgcc gacgacgaac
     3901 tgctccccgg cgttgccgcg ctgttcaacg gggcgttgtt ccgggctgcc accctcgacg
     3961 tcgtgggggt cccggacctc cggctgttcg tccggggcga cgaggtggag atgcaccgca
     4021 ggctggtgcg gtcgaacctg ccgttcggca cgtcgctgcg ggcgacgtac ctgcacccgg
     4081 ccgcctcgga cgacgacaag ccgatgctct gtggccggat gcacgcccgt gaccccgacg
     4141 atccggtgaa gcgctactac acctaccgca accgcgggta cctcgtctcg caaccgggga
     4201 tgcggaaggt cgggctgctg gaggtgccgc ggttcgcctg gtacttcctg gtgacgaaac
     4261 gcagcccgcg gcagttcgcc cagtgggtgc ggctggtgcg gcaggggcgt gcggagcggt
     4321 tccaccgcgc ctgacctccc cggcgtccgt tcacggcacg ccaccacgta tcactcgatc
     4381 gtgtgtgatt aagatcacag cgaattcgac gcatctttct ctgctgttag tcgtgcgtct
     4441 agttcaatgt tcacggaatg cgactaacat ccctctcgta agcttccgac ctgcgacgaa
     4501 ggctgcctcg atcgatccag gtaaatactc ggttaactcc tcggctcacc actccaggtg
     4561 atcgtcgcct gtaccccgct gctaattttt ctcctcgata gtggccgaat cgggggatac
     4621 gcgatgtggg attggctccg ctttctggag cattggcaac tattcctgcc ggttggcgtc
     4681 atcggcggaa tctcgtggtc ggtctggctg atccgcaagc tcatgtcggc ccgctaccgc
     4741 ccgctgacga acgacttccg caccacgacg tccgtggtcg tcccgtcgtt ccgtgaagac
     4801 ccgaacgtcc tcatgcgctg cctcgacacg tggctggcgc aggggccgac cgaggtcatc
     4861 atcgtgctcg acatcgagga cctcgaagcc cgcgaccgcc tgatggcgcg ccaggaccgg
     4921 cgcttgcacg tcatgatgtt cgcccacgag ggcaagcggt cggcgctcgg tgtcggcatc
     4981 cgcgcggccc gccacgagat cctcgtactc accgactccg acaccgcgtg ggagccgggc
     5041 ctgctcggcg ccgtgcagat gcccttcgtc aactcgaagg tcggcgccgt cggcacccgg
     5101 cagaacgtcc acgagccacg cagcagcgtc tggcgccgcg tggccgactg gatcatcgac
     5161 ctgcggtaca tggactacgt gcccgcgacg gcccgccgcg gcggcgtcgt ttgcgtctcc
     5221 ggacgcaccg ccgcctaccg ccgatccgcg gtgatgccgg tcctcgcgaa cctcgagcac
     5281 gaatacttcc tcggcaggcg ctgcgtcgcc ggcgacgacg gcaggctgac ctggttggtc
     5341 ctcgcccagg gctaccggac cgaacaccag gactcggcgc gagccttgtc gatgttcccc
     5401 tccacgttcc gggcgttctg caagcagcgc gtccgctgga gccgcaaatc ctaccgctgc
     5461 tacctcaccg ccatctacaa gggctggttg tggagggccc cgctgatctc acagctgacc
     5521 gtgttccaga tcctgttcac cccggtcacc atgttcgcga cggtctggta cctcgtcgcg
     5581 gcctggttca gcccgcaacg cgagctcgcc gtgatcctcg gcctcgcgtg gctcttcatc
     5641 ggtcgcgcca tccgcagctt ctcgcacctg cgccgcagac cgtccgatgt cgtcatcctg
     5701 ccgctcgtca ccctagtgat catgttcgtg gctctaccga tcaaggcctg ggcgttcgtc
     5761 accatgaaca agcaggggtg gctcacccgg cgtgccgatc tcgtcggcgg cgagggccag
     5821 gacgaggcca gcctgacgga ggacagccgt ggcgcaggct gatcccccga ggcgcagcgc
     5881 agggcgccgc tggctcgtgc tgctgaccgc gctcgccgtc gtcggcggca tgggggtcgc
     5941 ggccgtccag acgggcctgg tcgatcgcgt cgtcaccggc ctcggcccgg tggacgccgg
     6001 cagcaggctc gacgacgccg aggaggtcgg agccgtggtg ccgccgaccg acgccgagga
     6061 tgccgctccc acgaccgagg cgcagcagcg ccaggccacg ctcgtcgagg ccgaggacga
     6121 ccggctcttt gccatcgtca gcgacaggcc gccctcggac gtcccctacg aggtggctgc
     6181 cgcaggccag gccgttccca ccgtggtgct cacggcccgc tccaatccct acgacctccc
     6241 ggcgctggag cgcctcgggg ccgcggcgag gctgcccgat gggtcctggt tgctgagccg
     6301 ctcggtggtg gtcgcacgcg gcgccgagct gcggatccag gagccggggg cgacgctgcg
     6361 gctggccagc ggaccaagcg ggttcgcgac gctggtcgcg ttcaagggga ccctgaccct
     6421 ctcgggcggt cccgacgcgc cgctgacact caccagttgg gacccggcga cgaacgccgc
     6481 agacgccgag cgcggcgacg gccgggccta catccgtgcg gtcggggcgc ggatggacct
     6541 cacggcggtg cacgtctccg acctcggttt ctggagcggt cgtaccggcg gtgtggcctg
     6601 gaccgggagc gcgggtgctg cggccaccgg gtcggcaaac cacacgacga tcgagcgcgg
     6661 ccactacggg atcttcacca gccgaaccaa ggacgccgtc gtcaacgaca cgatcctgcg
     6721 cgacaacgag ctggacgggc tgctcctgca ccgcgagacg agcggcctcg ttgccacggg
     6781 cgtcacaagc accggcaacg gacgcgacgg catcaccgcg acgcggggcg cccagcgcat
     6841 caccctctcc caggtcacgg cctcggacaa cgcaggtagc gggatccgga tcgacggttc
     6901 cccgctggcc gccgaggcga ccgccggcgg ggccagcacc gccccgggca ggggctacac
     6961 gatcgagcgc tcgaccgcgg cgggcaacac cgaggtgggg atcctcgccg tcgaagccgc
     7021 cgggctcgta ctgcgcaaca actccgtctc ggggaaccgc gacggcatcg tggtccgtgg
     7081 cggcacggac cggccgaccg tctcgggcaa tgccgtggat gccgccgagt tcggcattgc
     7141 ggtgcggagc ggcgcccggg acgcgcaggt cagcggcaac cggatcggct cggccgtcat
     7201 cgcgatccag gtcacggacg cgtcggcgaa gatccatcag aacgaggtcc gcgccgcgtc
     7261 ccggtacggc gtctcgttgg tcggtgaggt gaccgggtcc tcggtcgccg ccaacaagct
     7321 ggcaggccgt gggttgtcgg ctctcgacgt gaaccgcgtc ggcaccaccg ccaccgtcgc
     7381 catatccggg aacgacgagg cccggtggaa tgtcgatcgg gacgagctgc gctactggcg
     7441 tgactacgcc gtggaccatc cgctgctgct gctgtggctg ctcatcctcc tgcttccgct
     7501 cgccgcccgg gtgtggacca agcgccgcag gcggatccgc gacgagcacg cccaccccta
     7561 cgtcaacgtg ccgccagggc cgccggtccc cggcaacagc aacgtgatca cgtcaatcgg
     7621 caggccggcg ccgaccgcgc cacgcccgcc cccgccggtg gtcccgcccg cggcgacgcc
     7681 cccaccccag gacgtcaccg cgaccgtcgc gctgccggtc acgcgtgtca cggtggtctc
     7741 cggacgagga gcccgccggt gacgcccccg cccgatgagg tcacccgggg ccgattccac
     7801 cgccgggtca accggccccg ggtcacctcg atcctcggcg gcctggtcgc ggtgggtgtc
     7861 ctggccggcg tcgccgcctg cggcgccggt ggtggtaccg gcccaccgcc acccccgaca
     7921 actgcgccgc tgccggcggc cggaacggca tgcacgcaga accccaccac tgaggtccac
     7981 aacgccgacg agctcaccga ggcgctcgcg ggtgcacgta cgggatcggt gatccgcatg
     8041 gccgcgggca cctatcgagg catgttcgtc gccacagctc agggcacccc ggacaagccg
     8101 atcgccctct gcggcggccg cgacgcgatt ctcgacgggg gcaccaccga cggtggctac
     8161 gcgttccatc tcgacggcgc cacgcactgg gagctgagcg gttttacggt ccgcggcggt
     8221 cagaagggcg tgatggccga tcgcacccag ggcaccgtca tcgacggcct cctcgtcgag
     8281 ggcaccggcg acgaggcgat ccacctgagg cagaacagca ccgacaacgt cgtgcggggc
     8341 aacaccgtgc aacgcaccgg cctacgcaac ggcaagttcg gcgagggcat ctacatcggg
     8401 agcgccgaga gcaactggtg cgagcagacc aactgcgagc ccgaccgcag cgaccgcaac
     8461 gtcatcgagg gcaacaccat ctccgggacg acctcggagt cggtggacat caaggaaggc
     8521 accaccggcg ggatcctccg cggcaatacg ttctcgggcg ccgggatgac cgacgccgac
     8581 tcgtgggtgg acatcaaggg gaacggctgg tcgatcgtcg gcaacgtcgg caccgacagt
     8641 cccgaggacg ggttccaggt ccacgagatc ctcgacggct ggggcctgga caacaccttc
     8701 gactccaaca ccgccacggt caacgcggat ggcttcgcga tcaacgtcac caagaacaag
     8761 gaacggaacc gggtcggctg cgacaaccgc ggcatcgacg ccggtcaggg cctgagcccc
     8821 gaagcctgcg gctgatgccc acgaccctcc cggaccatcc gaatcgaatc gagaggagcc
     8881 gctgtgttcg ctcgacggat cgccgtcatc ggcaccggct acgtaggact gacgactggc
     8941 gcgtgcctgg cctcactggg ccatcacgtg gtgtgcgccg acatcgaccg cgacaaggtc
     9001 gaccgcctga acgacggcca gatcgacatc ctcgaacccg ggttgcccga gctggtctcc
     9061 gaaggcgtcg ctgcgggccg tctcgagttc gtcctcggcg cccggcaggc gatcgtccgg
     9121 ccggacgagc ccgccgtcga ggtggtgttc ctctgcgtcc cgacgccgat gggcgagggc
     9181 ggggccgccg acctcgccgc ggtgcaggcg gtcgcgaacg aggtgcgcca cctgctgccg
     9241 aaggactgcg tcgtggtcaa caagtcgacg gtcccggtcg gcacggcaga ggcgacccgg
     9301 aagatgctgg gcaggcgcga cgtgtcggtc gtcagcaacc cggagttcct cagggagggc
     9361 agcgcggtcc acgacttcct gcaccccgac cggatcgtgg tcggcagcga cgcccaggac
     9421 gccgcggagc gcgtggctgc cctgtactcc aagctcggcg cccccaccca gctcaccgac
     9481 gcagccagcg ccgagatggt caagtacgcg gcgaactgct tcctggcgat gaaactgtcc
     9541 tacgtcaacg cgctcgccga cctgtgtgaa cgcctcggcg cggacgtgat cgaggtcacc
     9601 gagggcatgg gttacgaccg gcggatcggg cagaccttcc tgtccccggg ccccggctgg
     9661 ggaggctcgt gcttcccgaa ggacacccat gccctgctgc agatcgctgc ggcggccggg
     9721 atggacttcg agctggtcgg ggcgagcctc cggaccaacg agacgacgcg cctgcgggtg
     9781 gtcgagaagg tccgcgcggc tgtcgggggc tcgctcgccg ggcagcggat cgggctgctc
     9841 gggctcacgt tcaaggccgg caccgacgac ctgcgcgact cacccgcgct cgccgtcgcg
     9901 gcgctgctgc gcgcggaggg cgccgagctg accggttacg acccggcctg cccgcaggac
     9961 gtccccggtg tgaccgacgg gatcatcgtc gtcgacgacg cgctggacgc ggccaaggac
    10021 gcggttgcgc ttgtcgtgct caccgagtgg cagcagttcc ggtcgctgga ctggaccgca
    10081 ctcggcggtg tggtcggcgc gcccgtggtg atcgacaccc gcaacctgct cgaccccgac
    10141 gtgctccgcc gcgccgggtt cgcgtggatc gggctcggcc gcagctgacc cacgaccggt
    10201 ccggcggcgc ccggccgtcg ggccggaacc cgtgggacgt gtggtcagcg gcggaagacg
    10261 accgtgcgga gcatgacgaa gttgatggcg gtggcggcgc cctgggcgat cacccaggcc
    10321 aggctgaccc gcatcgggat cgccggcagc acggcgagca tgagggcgtt cacgccgatg
    10381 ttcagcgcga acgtcgtgcc gtagagcagt acgaagccgg cgaaccgggc ccggcctgca
    10441 ccgtcgaccg tgaacgtgaa gcgccggttc agcaggtacg cggtggtggt gccgaggatg
    10501 aagctgatgg ccttcgcggc gtgtacccac gtgcccagcg cgagcagccc ctggtaggag
    10561 ccgaagtcga cgagggccga cagcacgccg atcgcgacga acctgctcag ctgcgcgacc
    10621 aggccgagct gcgccggtgc gggtcgggtc gcggtcaccg cagcagggta tcggccgggc
    10681 aggatcggcc ggcgaccgcc atcgccccgc cgatgccggc ggacgggcgc ggatgcgaga
    10741 gcttgtgtcc tcccggcacg atcatcgggt gaccaccgtg accgatgccg acacccccac
    10801 cggggtacgc gccctcgccg acgactacgt ccgccgcctc gccgacctcg acaccagagt
    10861 cgcgaccatg ctgggtaccc atcccggcga cgaccgggtc cccaacctct ctcccgacgg
    10921 ccagagtgcg accgacgagc tcgcgcgaag ggtgctcacc gcccttccca ccgctgtggt
    10981 ggccgacgag gatgatcgac gctgcgcccg cctgctgcgg gagcgcctcg aggcccagct
    11041 cgccgtgagc gagactggcg agcacctgcg cgcggtgcgc aacatcgtgg gcccccacca
    11101 gcaggtacgg cagctcttcg agctgatgcc gacggccacg tccgatgact gggcggtggt
    11161 ggcgcgccgg atggcgaagg tgccccaggc ctactcgggt tacgtcgcat cactcaccga
    11221 gggatcccgc cgcgggttgc acgccgcgcc ccgccaggtc gagacggtca tcggccagct
    11281 cgacgcctgg ctcacggacg cgggcgggtc ctggttccag gcgttcgtcg caaaggcggc
    11341 cgacgtcacc ggacccacgg tgcgcgacgc gctgcacgaa ggagcaggcg ccgccacggc
    11401 tgccgccgcc gagctccggg atttcctcgc ccgggagtat ctgcccgcgg cgcagggcac
    11461 gccggacggc gtcggcgcgg agcgctaccg gatcggagcg cgcgtgagca ccggctcgga
    11521 cctcgatctc gccgaggcct acgcctgggg ctgggacgag ttccgccgcc tggacgcgga
    11581 gatgcgccgc gaggcggaag gggtactggc aggcgccggc cccgtcatgg cgatgcgtca
    11641 cctcgatgag aacggtgcgg cggtcgacgg cgtcgacgag gtgcgcgcct ggctgcagca
    11701 gatgatggac accgcgatgg acgagctgga cggtgtgcac ttcgacatcg ccgcgccggt
    11761 tcgcaccgtc gaggcgatga tcgcgccgtc cggcagcgcc gcggcgccgt actacacgcg
    11821 cccgtcgctg gacttctccc ggcccggccg gacgtggctg cccacgctgg ggcagcaccg
    11881 gttcccgctg tggggtctga tcagcacctg gtaccacgag ggcgtccccg gccatcatct
    11941 gcagctcgcg cagtggacgc acctcgccaa ccggctttcg acgtaccaga cctcggtcgg
    12001 ctcggtgtcc ggcaacaccg agggctgggc actctactcc gagcggctga tggacgagct
    12061 gggctacctg accccgcccg gtgcgcggct cgggtatctc gacgcgcagc agatgcgcgc
    12121 cattcgggtg gtgatcgaca tcggtatgca cctcgggctg caggttcccg ccgactcgcc
    12181 gctgctcgcc ggggagacct ggacgccgga ggtggcgcgt caattcttcg gggcccactc
    12241 cggccgtccc ggcccgttcc tggacagcga gatcgtccgg tacctgggct ggcctggtca
    12301 ggcgatcagc tacaagctcg gcgagcgggc gtggctggcc gggcgggatg ccgcgatgac
    12361 cgctcgcggt gcggacttcg acctcaaggc gtggcatgcc gccgccctgt ccttgggcag
    12421 cctgggcctg gacgacctga cggccgagct cgcccgcctg taggacgtcg gagccgcctc
    12481 agaagtagtg gccggcgaag tggtagcgcc tgcgcacaga caccgagccc atcccggcgc
    12541 ggccgtgcac cacgaaatgg gggacgccgg ggcgacgggt ggcggggacg ctgctcttga
    12601 cggtgcccat gctggccacg acgcggtcga tgttggcggt ggactcgtcc ggcacgagga
    12661 gccgcgcgga gcctgcgccg aggtccacct cgatgtcgat gacgtcgtac ggcagggctg
    12721 tgtcgcagaa gtcgaggacg acggagccca tggcgctctg caggcgcagc cgcggcggga
    12781 cgtcccaggc tccgcggcgc ttgatggtcg aggctccggt gcgcaacacc aggggtggtg
    12841 cgtccggcgc gctctgcggg gcggacgccg ggacggcgag ctgatgggcg ccgggcagat
    12901 cggccagtgg cggacggaga tcggcttcgt accgcgccgc gtagaccacc gacagccgct
    12961 cctccagctc ggaaacggtg atgcggccct cggcgagcgc cttgtggagc tgggcggcgg
    13021 ctcgctcccg gtcggcgtcg gagatcctga ttccgggcgg atccgggggc gtcgtactca
    13081 cgttcgcgag gatagtgccg atacatgccg tcgccagacc ttcagtccga cgtcggcggg
    13141 gttcggagct ggagtgtgaa cgccggtcgt cgaacaccgt gacgaccgtg ggcacgacgt
    13201 cgccgcgcgg cgcccgcctt gagctgcaac gtcacctgac gaatgcgtct cctggccggc
    13261 atgagaggga gcgtgggatc tccagcgggt ggaggctcaa gcgcaatatc gacggggagt
    13321 agcctgaccc cgatgtccgg aacggcgagc ctgcgcggat gggggcgcac cgcgcccacc
    13381 acggcgcagg tgcacgaacc caggtccgtc gatgatgtcc gcgcggccgt ggcggcggca
    13441 ggcccacgcg ggatcatcgc ccgcggcctc ggccggtcct acggtgatcc tgcgcagaac
    13501 gcgggcggca cggtgctcga catgaccggc ctcgccacca ttcactccat cgacgccgac
    13561 agcgggatcg cggtggtcga cggcggggtc agcctcgaca cgttgatgcg tcgcgccctg
    13621 ccgtacgggc tctgggtacc tgtcctgccg ggcacccggc aggtgacgat cggcggggcg
    13681 gtcggcgccg atgtccacgg caagaaccat cacagcaagg gcagcttcgg gaaccacgtc
    13741 ctgtcgctgg acctggtcac cgccgatggt gcgctccgca ccctcacccc ggacggcccg
    13801 gacgccgagc tgttctgggc cacggtgggc gggatgggtc tcacgggtgt gatcgtgcgt
    13861 gccacggtgc agctgcaccg cacggagtcg gcctacttcg tggtcgacac tgaccgcacc
    13921 gccgacctcg acgagctgat ggggttgctc accgacggct ccgacgacac ctacggctac
    13981 tccgccgcct ggttcgacac cacgaccacc ggcgcgcagc tcggccgcgc ggtgctcacg
    14041 cggggttcgc tcgccaccct cgaccagctg ccgcccaagc tgcgccacga tccgctgaag
    14101 ttcgaggcgc cgcagctgct gtcgttcccc gacgtgtttc cgaacgggct ggccaaccgg
    14161 atcacgctgc gcgccttcag cgagctctgg taccgcaagg ccccgaagcg ccagcgcggg
    14221 tcggtgcaga acatcacgac cttctaccag gtgctcgacc tgttcggcga ctggaaccgg
    14281 gtctacggat cgcgcggttt cctgcagtac cagttcctcg tcccgttcgg cgaggaaccg
    14341 acgttccgca aggtcgtcga gaagatcgcg cagtcgccgc acgcgtccgg gctcaacgtg
    14401 ctcaagcgct tcggcgaggg caatgcggcg ccgctgtcgt tcccgcagcc gggctggacg
    14461 atcaccgtcg acttcccgat cgcgcgcggg ctgcaccgct tctgcgagga gctcgacgag
    14521 ctcgtactcg gcgcgggcgg gcggctgtac ctggccaagg agtcgcgcgc gagcgccgcg
    14581 accatccgcc gcggctaccc ccggttcgag gagtggcgca aggtacgcga cgcggccgat
    14641 cccaccgggg tcttcacatc cgacatggcc agacggctgg agctgacgaa ctgatgatcg
    14701 acgccgtggg caacccgcag agcgttctgc tgatgggcgg aacctcggag atcggactgg
    14761 ccgtcgtcga ggcgttcgcc gccgaccggc cgctccgcgt ggtgctggcc gcgcggccct
    14821 cggagcggct ggatgcggcc cgggcccgtc tcgagggtct cgggtgcgcc gtcgagacgc
    14881 tcccgttcga cgcacgcgct ctcgacacac acgccgacgt ggtgcgcaag gcattcaccg
    14941 gcggcgacat cgacgtggcc gtcgtcgcct tcgggctgct cggcgacaac gagcaggcgt
    15001 ggaccgacgt cgagaccgcc gtcgaactgg cacaggtgaa ctacaccgcg gccgtgtcgg
    15061 tgggcgtcgc gctcgcggag cggctgcgcg cccagggcca tggggcgctg gtcgcgttct
    15121 cctcggttgc gggcgagcgg gcacggcggt cgaacttcgt ctacggctcc accaaggccg
    15181 gcatggacgc gttctactcc ggactcaccg aggcgttgcg cccgtccggt gtcggcgtca
    15241 ccgtggtgcg ccccggattc gtgcacacca ggatgaccga aggcaggaag cccgctccgt
    15301 tgtcgaccac ccccgaagcc gtcgccgccg tggtggtgga cgccgtacgc aaccgccgcg
    15361 agctcgtctg ggctccggct ccgctgcggg tgttgatgag cgtgctgcgg cacctgccgc
    15421 gcgcagtgtt ccgccgcctg cccgtctgat ccccggggga cggtcagcga cacccggaca
    15481 cgggtgaacc cgatgtcgag aacgatgcgg gtgctccgac ctgttcgcga cggtgcccac
    15541 cggcggcgcc gggcctgagg agtagacctg tgaagtacat gctgatcatg cagctgaacc
    15601 cggtcgcgtg ggacgcgctg tcccccgagc agcgcgacga ggtgatgaac ggccacggcg
    15661 ccttccttga cacgatcaag gcttcgggag agatggtcga gacccacgcc ctggccgaac
    15721 cggcgcagag cgccgtcgta cgcgtgcggg gcggagtccc ggccgtgacc gacggcccgt
    15781 tcctggaggc caaggagttc atgggcggct tctacatcgt ggagtgcgag acgcgggagc
    15841 gtgcgctcga gctcgccggc ctgatcccgg acgccggggt cgaagggatg gggatcgagg
    15901 tccggccggt catgttctcg gcgggccccg agtaacgcca aggccccgaa ggcctgtgct
    15961 cgagccgggc ggctcagacc tgggcgttgg ccgcggcgat gaagtgcccg accgaggagc
    16021 gcgggtcgcc tgcgcgaacc agcgcctcac ccaccagcac cacgtccgct ccggaccggc
    16081 ggtacgcggc gacgtcctcc ggcccggcga cacccgactc ggccacccga accgcatcct
    16141 ccgggatgtg cggtgccagc tcggcgaaga ccgagcgatc gaccttcagc gtcgtcaggt
    16201 cgcgggcgtt gatcccgatg acatccgctc cgacctcgat tgcacgttcg agctcagcgg
    16261 ccgtgtgtgc ctcgacgagg gcggtgagtc cgagctcgcg ggacagcccg agcatgtcgg
    16321 cgagcaggcc gcccccgagt gagaccacca tcagcagcgc caggtcggcg ccatgggccc
    16381 gggcttccca gagttggtac tgcgtgacca tgaagtcctt gcgcagcacc gggacgtcca
    16441 ccgacttcct tacggcgtcc aggtcggcca gcgagccacc gaaccgccgc tgctcggtca
    16501 gtacgctgat ggcagcggca ccgcccgcgg cgtactcacc cgcgagccgg gcagggtccg
    16561 gaatgtcggc cagcgcgccc ttgctcggac ttttccgttt cacctcggcg atgatcgata
    16621 ccccggtggt gcgaaaggct ggcaacggat ccagcggcgg cggcgcggcc gcggcacgcg
    16681 ccttcaactc ggcaagggaa acatcgttct gccgctgttc gagatcctcc cgaactccgt
    16741 cgagaatgcc gtccagaacg gacatctcgt cccctcgaaa tcgtgcttga actgcacagt
    16801 ggcctcgcga atcaacctac gccgggccct gacgttgcga agtgtgttcc ggctctcgtg
    16861 gcgctttcag ctctccacgt tcaggaaccc cccgcgcacg tatgcggcgc gttcgtagtc
    16921 accgcggtgt gccggtcgat cgcgctgggc gtcgatcagc gcggcggcga gtgtcggctg
    16981 acgcgtaccc gacgcggcgt ggcgcggcgc cggcgaggac aacctggcga acggggcggc
    17041 cccgtcaccg gtgccgctct cgcggctgtt cccggtggcg agggcccggc gcctggccgc
    17101 ggtgcgggag acggccgatg tgttgcccac ctccggccat ccggtgcctg cccctccgac
    17161 agcccggtgc cgagggggca cgggtggcgt caccatcggc gtcgacggcc caccgaccga
    17221 caccatccgg cgcatgcggc gccgggccgg ctcctccacc acgcggtatg cgagcgccgc
    17281 caccgtgagg gtcgacatca gcacgagcgc cccgatgacg tgcgccagcg gcgtatcggg
    17341 cccgagccag gagaacttct gcagcgccag ccagtagagc tcgaagatcg ggatgtgcac
    17401 gaggtagagg cagtaggaga tgcggccgcc gtacacggcc cactccgcgg acagcaccat
    17461 ggccgggccg cgatcggcca cggccagggc cccgaccaag agcgggaaca acgcgatcac
    17521 cgcgccgccc cggcccggcc cgacgagctc gccgagcagc aggcccgccg cgatcagcac
    17581 cggcagcacg acggcgagca ccgacgcgcc gagctgcatc ctggccgtcc ggcgcatctt
    17641 gcggacggcc aggtacatca gcgcgcctcc gctgaacccg cacaggatgc gcaccagcca
    17701 gctccacggg tagtacgggc tgccgctgct caggtatgcc caagccatcg gagccatcag
    17761 cgcaagcgcg gcgacgccca gcaccacggc gggcaggttg cggacccgga agagcacaag
    17821 cacggccagc gggaagagca ggtaggccag ccactccgcg ctgatcgacc aggtggaccc
    17881 cacccaggac gccccgtcga agaaggggtt gtcccagagc tggaccatga acagctgctg
    17941 gacgtactcg cccgggccga gctccggctg gacggcctgg aacgcgatgt tggagtcgga
    18001 cccgaacacc gcgcgggcca ccagccacag tccgaacagg tggaacacca cggcgtacgc
    18061 cggccacacg cggcaggcgc gcgcccacac gaaccgggcg gtggcacgcg cacgcagcgc
    18121 cggccccagg gcgtccagat aggtgtaggt gatcacgaac ccgctgagga cgaagaacag
    18181 gtcgaccccg agggcaccag aggtgatcaa cggccccagc acgtcgacca cgtcggtgac
    18241 ccccggcagc ggggtgaagt ggaaatggaa gacgacgacc cacgccgccg cgacgatccg
    18301 cagcccggtc agatttcgca gctccccgtg cagtgcacct cgagaaccgg acggggtggt
    18361 gggctccagg cctgctctcg tcacattgga aaaacgagaa ctcgctgacc agcggttcgc
    18421 gcgacggccg gaacgtaccg ggttggcctg cactgtcact gcccgctggc aagctgtacg
    18481 gccgaccggg tggcaccgac ccgtaccgtt tcagtacagc gtgcaactac cgcacacgtt
    18541 ccgctaccag cggtccaggt gcagcaccgt gtcgagcggc tctcgcgggg cgggcttgaa
    18601 cgtctctccg gtgtagtacg cggtgggcag gagtacgccc tgccggacgt tcggggggat
    18661 cccgagcagc tcggcgatct ccttctcgta acgcaggtgc agggtcgtcc aggcgctgcc
    18721 gagtccgcgg gcgcgagcgg cgatctggaa gctccacgc
//
LOCUS       DASRSC010000203        12812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_892115,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000203 DASRSC010000000
VERSION     DASRSC010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12812
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_892115"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1120
                     /locus_tag="VFE39_10080"
     CDS             <1..1120
                     /locus_tag="VFE39_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6258987.1"
                     /translation="LAVEVSGTGRHPAAWRRADAHPEALLTAPYHVGLAELAARSGAD
                     FVAVPDSFAPPGDDPDLVPLRLDAVAIAARVAPVVAGVGLVPSVTVTHTEPFHISKAI
                     ATLDIVSAGRAGWEPVASRTPAEAEQFGRKPAAPPEELWREAAAAVEVVVRLWDSWED
                     DAVIRDVATGRYVDRDKLHYIDFTGEFFSVKGPSITPRSPQAHPLVVLRGDEPEALPV
                     AGAWADVVRVAAADLAAAHSARERVRAAVAAAGRDPDTVAVLLDVEVLLGADPRSARR
                     ELDALDAHHEVAASSLRVVDTADALVDVIAEAVASGAADGLTVVPLALPSGLQAIAAD
                     VMPRLAGRGLRMPVPPGATLRDRFGLPRPANQFVEAKA"
     gene            1117..2487
                     /locus_tag="VFE39_10085"
     CDS             1117..2487
                     /locus_tag="VFE39_10085"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151993.1"
                     /note="This protein belongs to a clade of FMN-dependent
                     monooxygenases, within a broader family of
                     flavin-dependent oxidoreductases, the luciferase-like
                     monooxygenase (LMM) family, some of whose members use
                     coenzyme F420 rather than FMN; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NtaA/DmoA family FMN-dependent monooxygenase"
                     /protein_id="HET6258988.1"
                     /translation="MTGKKQIHLAAHFPGVNNTTVWSDPVSGSQTDFASFVHFAQTAE
                     RGLFDFLFLAEGLRLREHRGRIHDLDVVGRPDTLTVLNAIAAVTTHLGLGGTLNTTFN
                     EPYELAKQLASLDHLSGGRAAWNLVTSSDAFTGENFRRGGFLRPEQRYERAQEILDAT
                     RELWLSWAEDAVVADRERGVFVRDGGAPVVEHRGEQFSFSGRFSLPPTPQGHPVLIQS
                     GDSDAGREFGARTADAIFSRHGTLETGQAFYRDLKGRLPRYGRAERDVTILPATTFVL
                     GDSAEEAEALAAQVRRAQVSPQTAIAFLEQVWGRDLSDYDPDGPLPDVDPDPEGVSIT
                     RGRVRHEKDTVAVARQWRELAEAKGGLSIRELIIETTARQSFVGTPRQVAEQMDTYVQ
                     ENASDGFILIPHLVPGGLDEFVEKVVPELQDRGSYRTEYTGSTLRHHLGLARPTAGRL
                     AARAAG"
     gene            complement(2552..2756)
                     /locus_tag="VFE39_10090"
                     /pseudo
     CDS             complement(2552..2756)
                     /locus_tag="VFE39_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226387.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            complement(2759..3349)
                     /locus_tag="VFE39_10095"
     CDS             complement(2759..3349)
                     /locus_tag="VFE39_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6258989.1"
                     /translation="MIDVLQIAGAHHRYGALTALDSVDLTVRAGECVALLGPNGAGKT
                     TLVGLATGLLTPQAGRVRVCGGDPRRAATRRHLGVVQQSMGFPRTRLAARVLADDRRA
                     PRCGRGDPADHAHRGGGRRRRPGRGVAPRPCRGRRHPGRAHRPPTRPHDHRADLASRC
                     RAAGAARRALRDPGRRPGPGGHDRPGGRPPVVAGTR"
     gene            3473..4528
                     /locus_tag="VFE39_10100"
     CDS             3473..4528
                     /locus_tag="VFE39_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HET6258990.1"
                     /translation="MAERVLSTDELNRAVLARQLLLERSPLPPERAVEQVAGLQTQYA
                     PSGYIGLWSRLAGFRRDALTDALLQGRIVQGWVMRCTIHMVSARDYPPLTEAVRAARR
                     DWYARVARPAGVDMEALADAVRGHLAEGPLKQVEIQRLLAADGFPREAWSGAQLWLDL
                     VRVPPAGTWGTPRAHVYGLAEQTVPVPEPAPSVEDGQELLARRYLGGFGPASVKDVSS
                     FCGWTITATRRVLERLELRRFRDESGGELVDLPRAPLPPADTPVPVRFLGQWDASLLV
                     HARRAEILPEEYRPRVFARSIPQSVPTFLVDGHVAGTWRHVDGAVRCEPFHELPDGAS
                     REVKEEAERLTEFYARG"
     gene            complement(4540..4710)
                     /locus_tag="VFE39_10105"
     CDS             complement(4540..4710)
                     /locus_tag="VFE39_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258991.1"
                     /translation="MVTAIIVLICVALAVAISIIRPTGNFPAPTVVDRDRERVLADLR
                     ALPDYRCDVRLP"
     gene            4796..5761
                     /locus_tag="VFE39_10110"
     CDS             4796..5761
                     /locus_tag="VFE39_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6258992.1"
                     /translation="MLSIVAALVLPEVSPFEFGTICEVFGIDRSNEGVPPFELRVCGP
                     TAGEPVSSTVGMSVVPDHGLDGLRGADLVAVAATRQRSFPEPVLEALREAAAAGSTML
                     SVCSGAFVLGAAGLLDDRHCTTHWMNVDELRERYPRARIDPDVLFVDDGNLITSAGTA
                     AGIDACLHLVRRELGSAIVNTIARRMVVPPQRDGGQRQFVEQPVPRCSSDGLAPVLDW
                     VLDHLEVEHSVTDLARRARMSDRSFARRFVAETGTTPHRWLTLQRVLRAQRLLEDTDL
                     GMDEVAERSGFGGGALLRHHFRKVVGVAPADYRRTFAVRGAVAGR"
     gene            complement(5790..6884)
                     /locus_tag="VFE39_10115"
     CDS             complement(5790..6884)
                     /locus_tag="VFE39_10115"
                     /inference="COORDINATES: protein motif:HMM:NF021577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DICT sensory domain-containing protein"
                     /protein_id="HET6258993.1"
                     /translation="MDRSAGELLGKPALVEFSHAIEQFALAAAPGEPLIVIAMFQKLS
                     YFARALPVYEAIADRGAVTLVGMAEDVPPLLPPGIHHALVPASDSLAREWSITVLGPH
                     GGATLVAVDLETLDPVARTIEEGRQFCGRWSFRRDDAFHEVLRLRSQLRLTPAVVGQI
                     DAVLRAVQVRPEPAHQDWWGVPARFLAHRMEAAVRARTAAEVALEAVAEDRGERDPRT
                     GLYTEKFLDRWTAGLGAGTLPIGLALLRVFGIAEVRADYGLRAELAALHGVSGCVQDL
                     LTRVDRVVRIGREDFLVVLPSSPPEKVMWFCQEVCERVARLEDVYPFIALPGVAAATV
                     TRSRPLPIQQLVEQVELGGRESEPVSLLAD"
     gene            7263..8618
                     /locus_tag="VFE39_10120"
     CDS             7263..8618
                     /locus_tag="VFE39_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6258994.1"
                     /translation="MQQGQARVAVIGAGPSGLYAAAALLASGAPVSVEVLDRLPAPYG
                     LVRYGVAPDHVKMKSVIRVLQKPFDPAHVEFLGGVRVGDGGIPLDVLREHFHAVVHAT
                     GSSVDRRLGVPGEELAGSLGSGAFVSWYCGHPDHRDLLPLLDHADVAVVGAGNVALDV
                     ARVLAKTAEEMAETDVPEPVLDALRSSAVRDVHVLIRRGPQHVRFTPAELRQIGELAN
                     ADVVVHDDGLLAAGIEQPEDRRQKQNLQMLTEWAGRETTGKPRRVHLRFLRNPVRLLG
                     TGGRVSGVVVERTRIDDAGRVVGTGEEETLDVGLVVPAIGYDAEPIPGLPFDARTGTV
                     PNDGGRVVRDGVPVPGAYVTGWIKRGPTGVIGTNKSDAAETVAALLADLPGLPAPKSP
                     EPAAFRAALASYGMRPVDWTGWLRVDAEEIRRGGRRGAERVKVADLAEMLDAAHGVTT
                     P"
     gene            complement(8682..9251)
                     /locus_tag="VFE39_10125"
     CDS             complement(8682..9251)
                     /locus_tag="VFE39_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HET6258995.1"
                     /translation="MRAEQDELIPLKVLIAGGFGVGKTTLVRSLSEIPPLLTEQAMTA
                     ASIGVDDADAVPDKRTTTVAMDFGRITVDKSLILYLFGTPGQSRFWFMWDELARGAVG
                     AVVLVDLRRVDDCFPAIDYFENRRLPFVVGVNRFPGAEDYDEETVRDALALPADCPLV
                     WMDARNAASSSSALVTLVEHALARTAVVG"
     gene            complement(9241..9624)
                     /locus_tag="VFE39_10130"
     CDS             complement(9241..9624)
                     /locus_tag="VFE39_10130"
                     /inference="COORDINATES: protein motif:HMM:NF017170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HET6258996.1"
                     /translation="MEYPDPAGAGPVRGGRVVPVYALTGGRTRSGAGRDMPVETLVTM
                     TSAGGRTNDLQLEYRTTVELAARPISIVEIGAALGVPVGVARVLVSDLVDAGHLAVHL
                     PPQTVGGGGPAPQILERLLEGLRAR"
     gene            complement(9627..10028)
                     /locus_tag="VFE39_10135"
     CDS             complement(9627..10028)
                     /locus_tag="VFE39_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET6258997.1"
                     /translation="MSTRSGQLDWLLVEFARDVPGVVHGVVVSGDGLRLATSPGVGVA
                     LGDQLSAAASGLVSLARGTAHLLGAGPVSQTIVEMAGGYLFVSSVSQGATLAVFADRR
                     CDIGMIGYEMTMLANRVGHALTPAPRTSAGG"
     gene            complement(10025..12079)
                     /locus_tag="VFE39_10140"
     CDS             complement(10025..12079)
                     /locus_tag="VFE39_10140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6258998.1"
                     /translation="MARLLAYLPRGNLLDDKAWQRRHRLLQWVLLLHVPGLALFGLWL
                     GNRPAVVGYVLIAPVVALVGGYAIKQRRLASFFVTGGLVFCSAGLVVLTKGTIEAHFH
                     FFIIIGFIALYQDWVPFLWNVTFTVVSHGIGTIWLGNLIFATPAGQANPWLWSGIHGV
                     AVLAACVGMVIFWRITEDEQVEKEALGRQLVTADAEIGRRRFTSEMLVNLARRNQSML
                     YRQLDIINQLEEKEQDPDALSDLFTLDHLATRVRRNAENLLVLAGEQPPRTWSAPVPL
                     RDVVRAAIAETEDLDRVVFAVNEAIAVAGHAVADLTHLLAELTENAVRFSPPDTVVTV
                     RVRPDRRDEGAYTMTIEDWGVGMPPDDLAAANELLARPRDVDLAVAQRLGFHVVSRLA
                     ARHAVGVSLSTTPGSGVTAIVSLPAELFAETGDRPAPAPAAEDVVAVPRPYVPGHRFR
                     RPDGFATGPVRPVAADRRVGAESAAGVGRLSTESALPRPRHAADGAPAIASSPQSLST
                     QGSPPQYSPQAPSTPQGAPRAAEQGWVDGGWRGWWNPESAGAAEAGPANGQNATVDSR
                     TAVAEATPSNGATHGAQPAARLRPVPAPRQYSDSAPSGSGRRTPPGAAPSGGGPSLRR
                     RVPQSHLAPELRRPDAADDPAPVPQYDGAAASALSRYQASRKAAQAQVGEHPENRGGE
                     ST"
     gene            complement(12577..>12812)
                     /locus_tag="VFE39_10145"
     CDS             complement(12577..>12812)
                     /locus_tag="VFE39_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6258999.1"
                     /translation="RLQRRHEIVTTGGMDGVLRIGAALRACGFPVRDFTVEVRDGVPY
                     SSVTCTVSVTSDESATFAERIGAVPAVVSIAPC"
BASE COUNT         1790 a         4607 c         4658 g         1757 t
ORIGIN      
        1 tctggcagtg gaggtgtccg gcacgggccg gcacccggcg gcgtggcggc gggccgacgc
       61 tcacccggag gcgttgctca cggcgccgta ccacgtcggg ctggcggagc tcgcagcccg
      121 ctccggcgcc gatttcgttg ccgtgcccga ttccttcgcc ccgcccggcg acgatccgga
      181 cctggttccg ctgcgactgg acgcggtggc gatcgccgcg cgggtggcgc cggtcgtcgc
      241 cggggtcggg ctggttccgt cggtgaccgt cacccacacc gagccgttcc acatctccaa
      301 agcgatcgcc accctcgaca tcgtgtcggc cggccgagcc ggctgggagc ccgtcgcgtc
      361 gcggacaccc gccgaggccg agcagttcgg tcgcaagccc gccgcgccgc ccgaggagct
      421 gtggcgcgag gccgccgccg cggtcgaggt cgtggtgcgg ctctgggact cctgggagga
      481 cgacgccgtg atccgcgacg tcgccaccgg ccgctacgtc gaccgcgaca agctgcacta
      541 catcgacttc accggcgagt tcttctccgt gaagggcccg tcgatcacgc cgcgaagccc
      601 gcaggcccac ccgctggtcg tcctgcgcgg ggacgagccg gaggcgctgc cggtggcggg
      661 ggcgtgggcc gacgtcgtcc gcgtcgccgc tgccgacctc gccgcagcgc actctgcccg
      721 cgagcgggtg cgcgccgcgg tggctgcggc gggcagggac ccggacaccg tcgcggtgct
      781 gctcgacgtg gaggtgctgc tgggtgccga cccacgcagc gcccgccggg agctcgacgc
      841 gctcgacgcc caccacgagg tcgccgcctc gtcgctgagg gtcgtcgaca cggcggacgc
      901 gctggtcgac gtgatcgccg aggccgtagc gagcggcgcc gccgacggcc tcaccgttgt
      961 gcccctcgcg ctgcccagcg gcttgcaggc catcgccgcc gacgtgatgc cccggctcgc
     1021 cggccgcggc ctgcggatgc cggtcccgcc cggggccacg ctgcgcgacc gcttcggcct
     1081 gccccgcccc gccaaccagt tcgtggaggc aaaggcatga ccggcaagaa gcagatccac
     1141 ctggccgcgc acttccccgg cgtgaacaac accaccgtct ggagcgaccc ggtgtcgggc
     1201 agccagaccg acttcgcgtc gttcgtgcac ttcgcgcaga cggccgagcg gggcctgttc
     1261 gacttcctgt tcctcgccga ggggctgcgg ttgcgcgagc accgcgggcg gatccacgac
     1321 ctcgatgtcg tcgggcgccc cgacacgctc accgtgctca acgcgatcgc cgccgtcacc
     1381 acacacctcg gcctgggcgg caccctcaac accacgttca acgagcccta cgagctcgcg
     1441 aagcagctgg cctcgctcga ccacctctcc ggcggccggg ccgcgtggaa cctcgtcacc
     1501 agctcggacg cgttcaccgg cgagaacttc cgccgcgggg ggttcctgcg gcccgagcag
     1561 cgctacgagc gtgcgcagga gatcctcgac gccacccgcg agctgtggct ctcctgggcc
     1621 gaggacgcgg tcgtggccga ccgggagcgc ggcgtgttcg tccgcgacgg gggagcgccc
     1681 gtcgtggagc accggggtga gcagttctcg ttctcggggc ggttctcgct cccgccgacg
     1741 ccgcagggac acccggtgct gatccagtcc ggtgactcgg atgccggtcg ggagttcggg
     1801 gcccgcaccg ccgacgcgat cttctcccgg cacggcacgt tggagacggg ccaggcgttc
     1861 taccgcgatc tgaagggcag gctgccgcgc tacggacgcg ccgagcggga tgtcacgatc
     1921 ctgccggcca cgaccttcgt gctgggcgac agcgcggagg aggcggaggc gctggccgcg
     1981 caggtgcggc gggcgcaggt gagcccgcag accgcgatcg cgttcctcga acaggtgtgg
     2041 ggccgcgacc tgtcggacta cgaccccgat ggcccgctgc ccgacgtcga ccccgacccg
     2101 gagggggtgt cgatcacccg cggccgggtg cgccacgaga aggacacggt ggccgtggcc
     2161 cggcagtggc gcgagctcgc cgaggccaag ggcggcctct cgatccggga gctgatcatc
     2221 gagacgaccg ctcggcagtc gttcgtcggc accccgcggc aggtggccga gcagatggac
     2281 acctacgtgc aggagaacgc cagcgacggc ttcatcctga tcccgcacct ggtgcccggc
     2341 ggcctcgacg agttcgtcga gaaggtggtg ccggagctgc aggaccgcgg cagctaccgc
     2401 acggagtaca ccgggtcgac cctgcgccac cacctcgggc tcgcccgccc caccgccggt
     2461 cgcctcgcag cccgggccgc agggtgacgg ggttgctgag gagggtgttg aacaagtccg
     2521 cgcggtagca gagttgtcca gcgcgctcct caagccggcc ccggtcgcgg gcgacgcggg
     2581 cggcctcgat ccggttccgc accccgagct tgatgatcgc cgtggagagg tagttgcgca
     2641 ccgtcccctc ggccaggtgc agcttggcgg cggagcacgt cccgctcgcg gtcggtcatc
     2701 gggtcggcgc cgtcccatgc ggcgacggcg agcgccgggt ccaccacccg ctgcccgctc
     2761 atcgagtacc agccaccacc ggaggacgtc ctccgggccg gtcgtggcca cctggacccg
     2821 gtcgccgtcc cgggtcacgg agagcacgcc gggcagcgcc cgcagcacgg catcgggaag
     2881 cgaggtccgc gcgatgatcg tgcggtcggg taggcgggcg gtgagcccgg ccggggtgtc
     2941 ggcggccacg acacggccgt ggtgcaacac cacgacccgg tcggcgacgg cggcctcctc
     3001 cacgatgtgc gtggtcagca ggatccccac gcccgcatcg cggcgcgcgg cgatcgtctg
     3061 ccagaacgcg cgccgcgagg cgagtgcgcg ggaagcccat cgactgctgc accacgccga
     3121 ggtgtcgccg ggtcgccgcc cgccgcgggt ccccgccgca gacgcgcacc cggcccgcct
     3181 gcggggtcag cagcccggtg gccagcccca ccagcgtcgt cttgcctgcg ccgttcgggc
     3241 ccagcagcgc cacgcactcg cccgcgcgga cggtcaggtc caccgagtcg agggcggtca
     3301 gggcgccgta ccggtggtgc gcgcccgcga tctgcagtac gtcgatcatg ccgttcacgg
     3361 tgccgcgtgc gggccgggcg gggcagggga cggagtcagc ccctcgccct gacaattgtc
     3421 acgatcgtct gtgatcgggg acttgtcggt ggttcggggc acactcctga ccatggcgga
     3481 gcgcgtgttg tccaccgacg agctcaaccg tgcggttctc gcccgccagc tcctgctcga
     3541 acggtccccg ctgccaccgg aacgcgccgt cgagcaggtc gcggggctgc agacgcagta
     3601 cgcgccgtcg ggctacatcg ggctgtggtc gcgcttggcg ggtttccgcc gcgatgccct
     3661 cacggacgcg ctgctgcagg ggcgaatcgt gcagggctgg gtgatgcgct gcacgatcca
     3721 catggtctcg gcgcgcgact acccgccgct caccgaggcc gtccgcgcag ctcgccggga
     3781 ctggtacgcc cgcgtcgcgc gaccggcggg cgtcgacatg gaggccctcg ccgatgcggt
     3841 gcgcggccac ctcgcggagg ggccgctcaa gcaggtcgag atccagcggc tactggccgc
     3901 cgacgggttt ccgcgcgagg cgtggtcggg cgcccagctc tggctcgatc tggtgcgggt
     3961 gccgccggcg ggcacctggg gcacgccacg ggctcacgtc tacgggctgg cggagcagac
     4021 ggtccccgtg cccgagcccg caccctcggt ggaggacggg caggagctgc tggcccggcg
     4081 gtacctgggt ggtttcggcc cggcgtcggt caaagacgtc tcgtcgttct gcggctggac
     4141 gatcaccgcc acgcggcgcg tcctggagcg cctggagctc cgccgctttc gcgacgagtc
     4201 cggcggcgaa ctggtcgacc tgccgcgcgc cccgctgccg cctgccgaca ccccggtgcc
     4261 cgtccggttc ctgggccagt gggacgcgag ccttctggtg cacgcgcggc gtgcggagat
     4321 cctgccggag gagtaccggc cgcgggtctt cgcgaggtcg atcccccagt ccgtgcccac
     4381 gttcctcgtc gacggccacg tggccggtac gtggcgtcac gtcgacggcg ccgtccggtg
     4441 cgagccgttc cacgagctgc ccgacggggc ctcccgtgag gtgaaggagg aggccgaacg
     4501 cctcacggag ttctacgcca ggggatgacc acccggttgt cagggcaggc ggacgtcgca
     4561 ccggtagtcg ggcagcgcgc gcaggtccgc aagcacccgc tcgcgatcgc ggtcgaccac
     4621 ggtcggcgcg ggaaaattgc cggtcggacg gatgatcgag atcgcgacgg cgagtgcgac
     4681 gcagatcagg acgatgatcg cagtaaccat ggcagtaatt attcgagcat cgacttcctg
     4741 ccaccagtgg cagtcgcgcc cacgacagca aagatcctgc cattacactg gccgcatgct
     4801 gtccatcgtt gccgcgctcg tgcttcccga ggtctcgccc ttcgagttcg gcacgatctg
     4861 cgaggtcttc ggtatcgatc gcagcaacga gggggtgccg ccgttcgagc tgcgggtctg
     4921 cgggccgacg gcgggggagc cggtctcgag caccgtgggg atgagcgtcg tgcccgacca
     4981 cgggctcgac ggcctgcgcg gcgccgacct cgtcgcggtc gccgccaccc ggcaacggtc
     5041 gttccccgag cccgtactgg aggcgctgcg cgaggccgcc gcggccggca gcacgatgct
     5101 cagtgtgtgc tcgggagcgt tcgtgctcgg tgccgccggg ttgctcgacg accgccactg
     5161 caccacccac tggatgaacg tcgacgagct tcgcgagcgc tacccgcggg cccgcatcga
     5221 cccggacgtg ctgttcgtcg acgacggcaa cctcatcacc agcgcgggca cggcggcggg
     5281 catcgacgcc tgcctgcacc tcgtgcgccg ggagctgggc agtgcgatcg tcaacacgat
     5341 cgcccgccgg atggtcgtgc ccccgcagcg cgacggtggg cagcggcagt tcgtggagca
     5401 gccggtcccg cggtgctcgt ccgacggcct cgcgccggtg ctggactggg tgctcgatca
     5461 cctggaggtc gagcactcgg tcacggatct cgctcgccgg gctcgaatgt ccgaccgcag
     5521 cttcgcccgt cggttcgtcg ccgagacggg caccacgccc caccgctggc tgaccctgca
     5581 gcgcgtgctg cgtgcgcagc ggctgctcga ggacaccgac ctcggcatgg acgaggtggc
     5641 cgagcgcagc ggcttcgggg gtggggcgct gctgcgccac cacttccgca aggtcgtcgg
     5701 agtggcgccc gccgactacc ggcgcacctt cgccgtgcgt ggggcggtcg cgggccggtg
     5761 atgcgatccc gcggctgctc gggtgagcgt cagtcggcca gcaggctgac cggttcggac
     5821 tcccggcccc ccagctccac ctgctccacg agctgctgga tcggcagcgg cctgctgcgc
     5881 gtcaccgtgg ccgctgcgac gcccgggagg gcgatgaacg ggtagacgtc ctccagccgc
     5941 gccacgcgct cacacacctc ctggcagaac cacatgacct tctccggcgg cgaggacggg
     6001 agcacgacga ggaagtcctc gcggccgatc cggaccaccc tgtcgacgcg cgtgagcagg
     6061 tcctgtacac atccactgac cccgtgcagg gctgcgagct cggcccgcaa accgtagtcc
     6121 gcccgtacct cggcgatgcc gaagacgcgc agcagggcca gaccgatcgg caacgtgccc
     6181 gcgccgaggc ccgccgtcca gcggtcgagg aacttctcgg tgtagaggcc ggtgcgtggg
     6241 tcgcgctcgc cgcggtcctc cgcgacggcc tccagggcca cctccgccgc cgtgcgcgcc
     6301 cgcacagcgg cctccatccg gtgggcgagg aaacgggccg ggacgcccca ccaatcctgg
     6361 tgcgcgggtt cgggccggac ctgcacggca cgcaggacgg catcgatctg cccgacgacg
     6421 gccggcgtca gccgcagctg cgagcgcagc cggagcacct cgtggaaggc gtcatcccgg
     6481 cggaaggacc accggccgca gaactgcctg ccttcctcga tcgtccgtgc caccgggtcg
     6541 agcgtctcga ggtcgacggc gacgagcgtg gcgccgccgt gcgggccgag cacggtgatg
     6601 ctccactcgc gagcgagcga gtccgacgcc ggcaccagcg cgtgatgtat cccggggggc
     6661 agcagaggcg ggacgtcctc ggccatcccc acgagcgtga cggctcctcg gtcggcgatg
     6721 gcctcgtaga ccgggagagc gcgcgcgaag taggacagct tctggaacat cgcgatgacg
     6781 atcagtggct caccgggtgc ggcggcaagg gcgaactgct cgatcgcgtg cgagaactcc
     6841 accagcgccg gcttgccgag aagttcgccc gcacttcggt ccacgaggcg ggagggtagc
     6901 cgcgttgggg gcatcatcgt tgcgccgaac ggatgatcag tgggttcctg agtcgtggcg
     6961 cacaccgtcc tccgtgcgct cccgatcggg gatcgttcgc cgtaatgtcg ggacgtgagc
     7021 aagatgcgaa cgggtggggc cgctgtgctc accgagcccg attccgatcg cgccgtcgcc
     7081 ccgaccttca actgccccga gcggcacgcc ggcttggcgc cgggcccgga gatgttcgcc
     7141 gcagctccgc gcccctcggt ccacaggcag gcccgtgaga tcagcgttag ggagcactga
     7201 accacgtcct gaccggttcc cggtggcagg atcggatcag gaccggcaca ggagatgagc
     7261 gcatgcagca gggacaggct cgcgtcgcgg tgatcggagc gggcccgtct ggcctgtacg
     7321 ccgcggcggc cctactcgcg tccggtgcgc ccgtgagtgt cgaggtgctg gatcggctgc
     7381 ccgcaccgta cggcctcgtc cgttacggcg tggcgcccga ccacgtgaag atgaagtcgg
     7441 tcatccgcgt gctgcagaag cccttcgacc cggcgcatgt cgagttcctc ggcggggtcc
     7501 gggtcgggga cggcggcatc ccgctggatg tgctgcggga gcatttccac gccgtcgtgc
     7561 acgccaccgg tagctcggtc gaccgccgcc tgggggtgcc gggcgaggag ctggccggct
     7621 cgctcgggtc cggcgcattc gtcagctggt actgcggcca tccggaccac cgagatctgc
     7681 ttccgctgct ggaccacgcc gacgtggcgg tggtcggcgc cggcaacgtc gcactcgacg
     7741 tcgcccgcgt gctggccaag acggctgagg agatggccga gaccgacgtc cccgagccgg
     7801 tgctcgacgc gctgcgcagc agcgccgtcc gcgacgtcca cgtcctgatc cgccgcggcc
     7861 cgcagcatgt tcgattcacc ccggcggagc tgcgccagat cggcgagctc gcgaacgcgg
     7921 acgtggtcgt gcacgacgac gggctgctcg cggccggcat cgagcagccc gaggacaggc
     7981 ggcagaagca gaacctgcag atgctcacgg agtgggccgg gcgggagacc acgggcaagc
     8041 cgcggcgggt gcacctgcgc ttcctgcgca acccggtgcg gctgctcggc acgggcgggc
     8101 gggtgagcgg tgtggtcgtg gagcgcaccc ggatcgacga cgccggtcgt gtcgtcggca
     8161 cgggcgagga ggagacgctc gacgtcggcc tcgtggtgcc tgccatcggg tacgacgcgg
     8221 agccgatccc agggcttccc ttcgacgccc gcaccggcac cgtgccgaac gatggcgggc
     8281 gggtcgtgcg cgacggcgtc cctgtgcccg gcgcctacgt cacagggtgg atcaagcgcg
     8341 gcccgaccgg tgtgatcggc acgaacaaga gcgacgccgc cgagaccgtc gccgcgttgc
     8401 tggccgacct tcccgggctc cccgcgccga aatcccccga gcccgcggcg ttccgggcgg
     8461 cgctggcctc gtacggcatg cggccggtgg actggacggg gtggctccgg gtcgacgccg
     8521 aggagatccg tcgcggcggc cggcgtggcg cggagcgggt gaaggtggcc gacctggcgg
     8581 agatgctcga cgcagcgcac ggcgtcacga ccccctgaca gggttggcct tcgtcgtgcg
     8641 ctcgttgctg ctgtcgcgtc gggacctgga gttcctgtga ctcagccgac gaccgcggtg
     8701 cgggcaaggg cgtgttcgac cagggtcacc agcgccgaac tgctcgacgc cgcattcctg
     8761 gcgtccatcc agaccagggg gcagtccgcc ggcagcgcga gagcgtcccg gacggtctcc
     8821 tcgtcgtagt cctccgcccc cgggaaccgg ttgacgccca ccacgaacgg gagccgacgg
     8881 ttctcgaagt agtcgatggc cgggaagcag tcgtccacgc gacgcaggtc caccagcacc
     8941 acggcgccga cggccccgcg ggcgagctcg tcccacatga accagaaccg ggactgcccc
     9001 ggggtgccga acaggtagag gatcagcgac ttgtcaacgg tgatgcggcc gaagtccatg
     9061 gcgacggtgg tcgtgcgctt gtccggcact gcgtcggcgt cgtcgacccc gatcgacgcg
     9121 gccgtcatgg cctgctccgt gagcagcggt gggatctcgg agagcgagcg gacgagcgtc
     9181 gtcttgccca ccccgaaccc gccggcgatc aacaccttca gcgggatcag ctcgtcctgc
     9241 tcagcgcgca cggagtccct ccaacagtct ttcgaggatc tggggggcgg ggccgccgcc
     9301 gcccaccgtc tgcggtggca ggtgcaccgc cagatgaccg gcgtcgacca ggtcgctgac
     9361 caggacccgc gccacaccga cgggcacgcc gagcgctgca ccgatctcga cgatcgagat
     9421 cggccgcgcg gccagctcga cggtggtgcg gtactccagc tgcaggtcgt tcgtgcgccc
     9481 gcccgcggag gtcatcgtca cgagcgtctc caccggcatg tcccggcctg cgccggaacg
     9541 ggtgcggccg ccggtgagcg catacacggg cacgacgcgg ccgccccgca cggggccggc
     9601 gccggcaggg tcggggtact ccaccactac ccgcccgcac tcgtgcgcgg tgccggtgtg
     9661 agcgcgtgac cgacccggtt tgccagcatc gtcatctcgt acccgatcat cccgatgtcg
     9721 caacgccggt cggcgaacac cgcgagcgtc gcgccctggc tgaccgagct cacgaagagg
     9781 taaccgcccg ccatctcgac gattgtctga ctgaccggtc ccgcgccgag caggtgcgcc
     9841 gtcccgcgcg ccaggctcac gagtccggac gccgccgcag agagctggtc gcccagggcg
     9901 acaccgacgc cgggcgacgt ggccagacgg agcccgtcac cggagacgac caccccgtgg
     9961 accacgcccg gtacgtcgcg ggcgaactcg accagcagcc agtccagctg ccccgaccgc
    10021 gtgctcatgt ggactctcct cctcggtttt cggggtgctc cccgacctgc gcctgcgcgg
    10081 cctttcgact cgcctggtag cgggagaggg cgctggcggc agccccgtcg tactgcggga
    10141 cgggcgccgg gtcgtccgct gcgtccggac gacgcagctc gggggcgaga tgggactgcg
    10201 gcacccgtcg gcgcaggctc ggaccgccgc ccgagggcgc ggcgcccggt ggcgtccgac
    10261 ggcccgaacc ggacggggcc gagtcggagt actggcgggg cgcgggcacc gggcgcagtc
    10321 gggcagcggg ctgtgccccg tgcgtcgcgc cgttcgacgg ggtcgcctcg gcgaccgcgg
    10381 tccggctgtc caccgtcgcg ttctgtccgt tcgcgggccc cgcctcggcg gcacccgccg
    10441 actccgggtt ccaccagcca cgccaaccgc cgtcgaccca cccttgctcg gccgcgcgcg
    10501 gcgcgccctg cggcgtcgag ggggcctgcg gcgagtattg gggcggcgag ccctgcgtcg
    10561 acaaggattg gggcgatgac gcgatcgcag gagcgccgtc ggcggcgtgc cgcggccggg
    10621 gcagggcgct ctcggtgctc aaccgcccga cccctgccgc ggactcggcg ccgacgcgcc
    10681 ggtccgcggc caccggcctg accggtcccg ttgcgaaccc gtcgggacgg cggaaccggt
    10741 ggccgggcac gtagggacgc gggacggcga cgacgtcctc ggcggcgggc gccggagccg
    10801 gtcggtcccc ggtctcggca aagagctcgg ccggaaggga gacgatcgcc gtgacaccgg
    10861 aacccggcgt ggtgctcagc gacaccccca cggcgtgtcg tgcggcgagc cgggacacga
    10921 catggaagcc caggcgctgc gccaccgcca ggtccacgtc gcggggccga gcgagcagct
    10981 cgttcgcggc ggcgaggtcg tcgggcggca tgccgactcc ccagtcctcg atcgtcatcg
    11041 tgtacgcgcc ttcgtcccga cggtccgggc ggacgcgaac ggtgacgacg gtatccggcg
    11101 gcgagaagcg caccgcgttc tcggtcagct ctgccagcag gtgcgtcagg tcggcgacgg
    11161 cgtgaccggc caccgcgatg gcctcgttga ccgcgaacac cacccggtcg aggtcctcgg
    11221 tttcggcgat ggccgcgcgc acgacgtcgc gcagcgggac gggggccgac cacgtgcgcg
    11281 gcggctgctc ccccgcgagc accagcaggt tctccgcgtt gcgcctgacc cgggtggcca
    11341 ggtggtcgag cgtgaacagg tcggagaggg catccggatc ctgctccttc tcctccagct
    11401 ggttgatgat gtccagctgc cggtagagca tgctctggtt ccggcgggcg aggttgacca
    11461 gcatctcgga ggtgaaccgg cgccgcccga tctccgcgtc ggccgtcacc aactggcggc
    11521 ccagcgcttc cttctcgacc tgctcgtcct ccgtgattct ccagaagatc accattccga
    11581 cgcacgccgc gagcacggcg acgccgtgaa tccccgacca caaccacggg ttcgcctggc
    11641 ccgccggggt cgcgaagatc agattgccga gccagatcgt gccgatgcca tggctgacga
    11701 cggtgaacgt gacgttccag aggaacggca cccagtcctg gtagagcgcg atgaagccga
    11761 taatgatgaa gaagtggaaa tgcgcctcga tggtgccctt ggtgagcacc acgagcccag
    11821 ccgagcagaa gacgagtccg ccggttacga agaacgacgc aagccgccgc tgcttgatgg
    11881 cgtagccacc gacgagggcg accacggggg cgatcagcac gtagccgacg acggcgggcc
    11941 ggttgccgag ccacaagccg aacagcgcca acccgggcac gtgcagcagc aggacccact
    12001 gcagcaaccg gtgccgccgc tgccaggcct tgtcgtcgag caggttgccg cgcggcagat
    12061 aggccaggag acgggccaca ccctggccac caccttcgcg gccgtctacc cagccctgcc
    12121 caggaggacc attcggggta atgccctccc caggaggacc gatcggcgca atggcctgca
    12181 tcgagatctg ctccgtccgg ccgccacaac ggctcgtcgt tctcgtcgtc ggcggcgaac
    12241 ggtacaacta gctccagcgg atggaggacg gcggtatgct gttacgcaaa tggcgtagca
    12301 atattgcgct aaatggtcta cacccgttca gcggtaaccg gatccacgag tcgtgttatc
    12361 cgagtccgcc atcaacggcg atctcgcagg gtcggcacgg caggcccaga ccggcatcca
    12421 gggcctggac ctgcggggtc gcaaagccgt gatggccggc gcggcggggc tcaccctcct
    12481 gctggagcgg cgacactggc gaactctcga ccgcgccacg ctcgacgggc gccccgagtg
    12541 gctccagggg cgccggaggc ccagcgccag ggtgggtcag cagggtgcga tggacacgac
    12601 cgccggcacg gcgccgatgc gctcggcgaa cgtggccgac tcgtcgctgg tgaccgagac
    12661 ggtgcaggtc accgagctgt aggggacgcc gtcgcggacc tcgacggtga agtcgcggac
    12721 cgggaagccg caggcgcgca gcgcagcgcc gatgcgcagc acgccgtcca tgccgccggt
    12781 ggtcacgatc tcgtggcggc gctgcagacg gg
//
LOCUS       DASRSC010000204         8429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2694820,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000204 DASRSC010000000
VERSION     DASRSC010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8429
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2694820"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VFE39_10150"
     CDS             <1..899
                     /locus_tag="VFE39_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
                     /protein_id="HET6259000.1"
                     /translation="HELETTRSWELRRIDHPAVEPLLAYKELARLHSAHGWSWLRTWV
                     DGGRFRPEYVVGGVVSGRWASRGGGALQIPRAVRAAVRADPGWRLVVADAAQLEPRVL
                     AALSGDHALAPGEDDLYTALAAREFGGDRAKAKLALLSAMYGQTSGGAGVLLATLRRR
                     FPAAMGYVEDAARAGEEGRLVRSHLGRTCPPARPGHEQPAAARARGRFTRNFVVQATA
                     AEWALVLLAELRLQLRAARSPARLVFFQHDEVLLHTPAAAAEEAATAVRAAADTAGRR
                     LFGPSPVRFPMQLRIVEGYDER"
     gene            912..1133
                     /locus_tag="VFE39_10155"
     CDS             912..1133
                     /locus_tag="VFE39_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259001.1"
                     /translation="MQAQYSSCSRRHWYGAVCGRPAGDEALGLHHLDVETAGRVLDTV
                     VITDQRGPGRTSMSIVDSGVVKPLARTTS"
     gene            complement(1252..1559)
                     /locus_tag="VFE39_10160"
                     /pseudo
     CDS             complement(1252..1559)
                     /locus_tag="VFE39_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501311.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
     gene            complement(1668..2594)
                     /locus_tag="VFE39_10165"
     CDS             complement(1668..2594)
                     /locus_tag="VFE39_10165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6259002.1"
                     /translation="MRPRSGRWAFPLVLAAVMTVVAAATATADVPPAPSEQTPAQSYV
                     ALGDSYASGPLIPVQTGQPAGCLRSTNNYPAIVGRTLGVPGFQDVSCSGATTENLAGS
                     QSVRLGVNAPQFDALSKDTELVTLTIGGNDIGFSSIINECATRSPRQPAGAACRDFYT
                     AGGTDQLAERIDGTAPKIADALKEIGKRSPDARVLLVGYPAILPDTGPGCFPVVPFSP
                     GDVAYLRETEKRLNAMLADEAAAADIDFVDTYTPTIGHDMCQLPGTKWIEGLVPTAPA
                     YPVHPNALGMAGIAAVVVEALADETVPVAAGT"
     gene            complement(2723..3958)
                     /locus_tag="VFE39_10170"
     CDS             complement(2723..3958)
                     /locus_tag="VFE39_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HET6259003.1"
                     /translation="MNERPADIGELLRPLAPQVLGVVVRRHGQFDACEDAVQDALLAA
                     AVQWPVEGVPDNPRGWLITVASRRLADQWRSENARRRRESTAAALDGMDAAPAADVDR
                     AGAADDTLTLLFLCCHPALSPPSQVALTLRAVGGLTTTQIAHAFLVPESTMAQRISRA
                     KRTVAGTEFARPAEAERTERLGAVLHVLYLVFNEGYTATSGPDLQRPDLTREAIRLAR
                     QVHRLLPDDGEAAGLLALMLITDARRPARTRPDGALVPLAEQDRSRWDTAPIAEGVAL
                     ISATLARAPVGPYQLQAAIAAVHAEAGRAEDTDWPQVAALYRVLDVLAPGPVVTLNRA
                     VAVAMVDGPQAGLDLLATLDSDERIGGLHRVPAVRGHLLEMAGDEAAARSAYLEAARR
                     TTSVPEQRYLEDRAARLPL"
     gene            complement(3955..4305)
                     /locus_tag="VFE39_10175"
     CDS             complement(3955..4305)
                     /locus_tag="VFE39_10175"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6259004.1"
                     /translation="MKYMLLTYGIPAPGAHLEASGNGAEDTLLAEIVETGEWVDRAPL
                     ADPALTRTVRVLDGAVGTTDGPFTAVAEQLTGYWLVDCESMGRALELAALLAGTRTVA
                     VEVRPLMDPSGMEM"
     gene            4438..5976
                     /locus_tag="VFE39_10180"
     CDS             4438..5976
                     /locus_tag="VFE39_10180"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: protein motif:HMM:NF004829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HET6259005.1"
                     /translation="MSGVDQDGSPPVLIVGAGPTGLTTALLLARLGVACTVVDRHVGI
                     HGLPRAVHLDDEAVRILQRAGVAEAFARISSPATGLRLLDATLRPFAEFRRDRPVGDH
                     GHPESNLFDQPDLERILRAEVGRQPLVELREGVEVTTVRPSDLLVELRETCSGAARRV
                     CAEAVLGCDGAGSTIRPAIRARFRDLGFTERWFVLDVCCERPLPGWGGVDQVCDPRRA
                     ATFLPLPGNRYRWEFRMRPGETAAELARPERIAELTARWGTPASGLEVLRAAEYTFRA
                     RITDRWRHGRVLLLGDAAHLTPPFIGQGLGAGLRDAHNLAWKLAAVLGGADEDVLLDS
                     YQAERAPHAEAVIRGAVLVGRAMTGGQDIAAALRRPATGLLLRVPGVRAAAQRGIATR
                     YAPGPLVDRRRHRRDLAGTHCPQPTVLVDGHPSRLDDVLGDGYTLLAAGPVPPALRAR
                     AQRLGATVLQLRPTPEAAGRVQHDHGQLDDGGQLRAWLARGRAGAALLRPDRIVLATA
                     PHAP"
     gene            complement(6373..7452)
                     /locus_tag="VFE39_10185"
     CDS             complement(6373..7452)
                     /locus_tag="VFE39_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET6259006.1"
                     /translation="MSASAYIAGFGIAVPGGGLTQTEMWDGFFAQHFAGDRMAERIFR
                     GAGVRRRHAAVNPVVEDVSRWSTGERMRRYVAEALPLGKEAVAAALADAGLAPGDVGL
                     LAVVSCTGYVTPGLDIRVAADLGMASDVQRLFVGHMGCYAAIPGLGAAADYVVARGRP
                     AVVLCLELTSLHVQPAADDHGHVDRGQVVSHALFGDAAAAVVVLPNAADPRRVGGLEV
                     VEIAAVTDAASADHMTWDVTDLGFRMGLSPEVPRVLARHVSDMVSGLLGRHGLTTADV
                     DGWAVHPGGPRILSTVAERLGLPDDALDASLGVLAERGNCSSATVLLVLDALRTMRRP
                     RSRRATVAMAFGPGLTLYVALLRGT"
     gene            complement(7449..8222)
                     /locus_tag="VFE39_10190"
     CDS             complement(7449..8222)
                     /locus_tag="VFE39_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6259007.1"
                     /translation="MPDVRNGRIARLARNDPAQYDALARHWWRPRGPFAMLHWLAAAR
                     GALVPPAPREEAVLVDLGCGAGLLAPHVDAAGYRHIGIDVTASALAQAAAHGVTAVRA
                     DVAAVPLADGCADVVAAGEILEHVPDLAAVVAEACRLLRPGGLLVIDTLAATALSRFV
                     AVTLAERIPGAAPPGIHDPALFVDRAALVRECARHGVSLRLRGIRPSLVGLARWATGR
                     ADSVPMVSVPTTAVLFQAVGRRGQEEPGLTSGGGGDSST"
     gene            8249..>8429
                     /locus_tag="VFE39_10195"
     CDS             8249..>8429
                     /locus_tag="VFE39_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005438064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259008.1"
                     /translation="MRPLTAKAVALLRSCHPEPTVAVTLMVTALAVTTGRDVAGVLLV
                     AAAVLTGQLSIGWLND"
BASE COUNT         1110 a         3138 c         3058 g         1123 t
ORIGIN      
        1 ggcacgagct ggagaccaca cgctcgtggg agctgcgccg gatcgaccat ccggccgtcg
       61 agccgctgct ggcgtacaag gagctggccc ggctgcactc cgcccacgga tggtcgtggc
      121 tgcgcacctg ggtggacggc ggccggttcc ggccggagta cgtggtcggc ggggtggtgt
      181 cgggccggtg ggcctcccgc ggcgggggcg ccctgcagat cccacgcgcc gtgcgcgccg
      241 ccgtccgggc cgatcccgga tggcgcctgg tcgtggccga cgccgcgcaa ctggagccac
      301 gggtgcttgc ggcgctctcg ggtgaccacg cgctcgcccc cggcgaggac gacctgtaca
      361 ccgccctcgc cgcccgcgag ttcggcggcg accgcgccaa ggccaagctc gcgctgctgt
      421 ccgcgatgta cggccagacc tccggtggtg ccggagtact gctggccacc ttgcgcaggc
      481 ggttccccgc ggcgatgggc tacgtggagg acgccgcgag ggccggtgag gagggcaggc
      541 tcgtgcgctc acacctcggc cgcacctgcc cgcctgcgcg ccccggtcac gagcagccgg
      601 ccgcggcccg cgccaggggc cggttcaccc gcaactttgt cgtgcaggcg accgcggcgg
      661 agtgggcgct ggtcctgctc gcggagctgc gcctgcagct gcgggcggcc aggagcccgg
      721 ctcggctggt gttcttccag cacgacgagg tgttgctgca caccccggct gcagcagccg
      781 aggaggccgc gactgccgtc cgcgccgccg ccgacacagc cggccgccgg ctcttcggcc
      841 cgtccccggt gcgtttcccg atgcagctgc ggatcgtcga gggctacgac gagcgctgaa
      901 tcccaccagg catgcaggct cagtactcgt cgtgcagccg ccgccactgg tacggcgcgg
      961 tctgcggacg cccggcgggc gacgaggcgc tcgggctcca ccacctcgat gtagaaaccg
     1021 ccggtcgggt actcgacacc gtcgtcatca cggatcagcg tggcccgggg cggacgtcca
     1081 tgtcgatcgt cgacagcggt gtggtgaagc ccctggcccg aaccacgtcg tgacatgggt
     1141 ggggtcggtc cagacccgga agaccacctc tcgtggtacg tcgaggacgt gggtgctggt
     1201 gaactcccgc tccgcaggcc gtgccctgcg ccggcagcca tccgggctcc cctcctggaa
     1261 ctgccgcagg tggtcgtcga ggcggtcgaa gcgccctccc agaactcctg gcagcgctgc
     1321 acccagtcgg ctacgtcggc gagcggaccc gcatcgagcc ggcccggccg ccactgcgcg
     1381 gagcggctgc gcgtgatcag gccggcacgt tcgaggacct tcaggtgctt gaagatcgcc
     1441 ggcatgctga ccgcgaaagg ctcagccagc tcggtgaccg aggcctcgcc ccggctcagg
     1501 cgcgcaaggg tggcgcgccg ggcgcaaaag cacaaccgaa tggttgagga cgtgcacagc
     1561 tgcggaacgc cgcgttccgg tgcgtccctc agtcactcgc cacgcgggcg ctggccgcac
     1621 agttccggtt cgatgccggg ccgacgacgc agcgggccta gcggaggtca ggtgcccgcc
     1681 gccaccggga cggtctcgtc ggccagggcc tcgacgacca ctgcggcgat ccctgccatg
     1741 cccagggcgt tggggtgcac cggataggcg ggagccgtgg gcaccagacc ctcgatccac
     1801 ttcgtgccag gcagctggca catgtcgtgc ccgatagtgg gcgtgtaggt gtcgacgaag
     1861 tcgatatcgg ccgcggcggc ctcgtcggcg agcatcgcgt tgagccgctt ctcggtctcg
     1921 cgcaggtagg ccacgtcacc agggctgaac gggaccaccg ggaagcagcc gggtccggtg
     1981 tcggggagga tcgccgggta gccgacgagg agcacccggg catccgggga gcgcttgccg
     2041 atctccttca acgcgtcagc gatcttcggt gcggtgccgt cgatccgctc ggcgagctgg
     2101 tcggtgccgc ccgcggtgta gaagtcccgg cacgcggccc cggccggctg ccggggggag
     2161 cgggtggcgc actcgttgat gatcgagctg aaaccgatgt cgttgccacc gatcgtgagc
     2221 gtcaccagct cggtgtcctt cgacagcgcg tcgaactgcg gggcgttgac gcccagccgt
     2281 acggactgcg agcccgcgag gttctccgtg gtggctccgc tgcagctcac gtcctggaaa
     2341 ccgggcacgc cgagcgtgcg ccccacgatc gccgggtagt tgttggtgga gcgcaggcac
     2401 ccggccggct gcccggtctg caccgggatt agcggaccgg acgcgtagga gtcgccgagc
     2461 gctacgtagc tctgcgccgg cgtctgctcc gacggagcgg gcgggacgtc cgcggtcgcg
     2521 gtcgccgcgg ccaccacggt catgaccgcc gccaggacga ggggaaaggc ccagcggccg
     2581 ctgcgaggac gcacggtatc ccaacgacga caacacgagt gggttactca ccgccggcgg
     2641 gttggcgtcc tcgcacggtg gctgtcgtcc tcgtggctgt cgtcctcgca gcgtccccag
     2701 cctctgcgaa gacgccgggc tgtcataggg gcaggcgggc agcgcggtcc tcgagatacc
     2761 gctgctccgg cacgctggtg gtgcggcggg ctgcctccag gtaggcggac cgggctgctg
     2821 cctcgtcccc cgccatctcc agcagatggc cgcggacggc cggcacgcgg tggagccccc
     2881 cgatccgctc gtcggagtcg agcgtcgcca agaggtccag gcccgcctgc ggaccgtcca
     2941 ccatcgcgac ggcgacggcc cggttgagcg tgacgaccgg gccgggcgcg agcacgtcca
     3001 acacccggta gagcgcggca acctgcggcc agtcggtgtc ctcggcgcgc cccgcctcgg
     3061 cgtgcacagc cgcgatcgcc gcctggagct ggtaagggcc gaccggcgcg cgggccagcg
     3121 tggcgctgat cagcgcgacc ccctccgcaa tcggcgcggt gtcccagcgg ctcctgtcct
     3181 gctcggccag gggcacgaga gcgccgtccg ggcgggtgcg ggcgggcctg cgggcgtccg
     3241 tgatcagcat cagggcgagc agcccggcgg cctcaccgtc gtcgggcagc agccgatgca
     3301 cctggcgagc cagccggatc gcctcgcggg tgaggtcggg acgctgcagg tcaggaccgg
     3361 aggtggccgt gtagccctcg ttgaagacga ggtacagcac gtgcagcacg gcaccgagcc
     3421 gttcggtccg ctcggcctcg gccggcctgg cgaactcggt gccggcgacc gtgcgcttcg
     3481 cccggctgat ccgctgcgcc atcgtcgact cgggtacgag aaacgcgtgc gcgatctggg
     3541 tggtggtcag cccgccaacg gcccgcaggg tgagcgctac ctgcgacggg ggcgacagcg
     3601 ccgggtgaca gcacaggaac agcagggtga gcgtgtcgtc cgccgccccg gcccggtcga
     3661 cgtcagcggc aggtgccgcg tccattccgt cgagggccgc agccgtgctc tcgcggcgcc
     3721 tgcgcgcgtt ctcgctgcgc cactggtcgg ccagccgccg cgaggccacg gtgatcagcc
     3781 agccgcgcgg gttgtcgggc acaccctcga ccggccactg caccgcggcg gcgagcagcg
     3841 cgtcctgcac cgcgtcctcg cacgcgtcga actggccgtg ccggcgcacc accaccccga
     3901 ggacctgcgg cgccagcggg cgcagcagct ccccgatgtc cgcgggccgc tcgttcacat
     3961 ctccatcccg gacgggtcca tcaacggacg gacctcgacc gcgacggtgc gggtcccggc
     4021 cagcagggcg gcgagctcga gggcccggcc catgctctcg cagtcgacca gccagtagcc
     4081 cgtcagctgc tccgcgacag cggtgaacgg tccgtcggtg gtccccaccg caccgtcgag
     4141 cacccggacg gttcgggtca acgccggatc ggccagtggt gcgcggtcca cccactcccc
     4201 cgtctccacg atctccgcca gcaaggtgtc ctccgcgccg ttgcctgagg cctccagatg
     4261 cgcacccggg gcgggaatgc cgtaggtcag cagcatgtac ttcatctggc gctcctcctc
     4321 ggccgggcgc ggcgggtgct gcgctcctca ccgaggacgt cggagccgtg cggctgccct
     4381 cgacatgacg gagcctacgg cccctgtcca tgtcgcagct gtgcttggct gacggaggtg
     4441 agcggtgtgg accaggacgg cagcccgccg gtactgatcg tcggcgccgg tcccaccggg
     4501 ctcaccacag ccctcctgct ggcccggctc ggagtggcct gcacggtcgt cgaccggcac
     4561 gtcgggatcc atgggctgcc ccgtgcggtg cacctcgacg acgaggcggt gcgcatcctg
     4621 cagcgggccg gcgtcgccga ggcgttcgct cgcatcagca gcccggcgac gggtctgcgg
     4681 ctgctcgacg cgacactccg gccctttgcg gagtttcgcc gtgaccggcc ggtcggcgac
     4741 cacggccacc ccgagtccaa cctgttcgac cagcccgacc tcgaacggat tctgcgcgcc
     4801 gaggtgggcc gccagccgct ggtggagctg cgggaggggg tcgaggtgac caccgtccgg
     4861 ccgtctgacc tgctcgtcga gctgcgggag acgtgctcgg gcgccgcacg gcgggtgtgt
     4921 gcggaggcgg tactcggctg cgacggcgcc ggcagcacga tccgtcccgc catccgggcc
     4981 cgcttccgcg atctggggtt caccgagcgg tggttcgtcc tcgacgtctg ctgcgagcgg
     5041 ccgctgccgg gttggggcgg tgtcgaccag gtctgcgacc cccgccgcgc ggcgaccttc
     5101 ctcccgctgc ccggcaaccg gtatcggtgg gagttccgga tgcgtcccgg tgagacagcc
     5161 gcggaactcg cacggccgga gcggatcgcc gagctcaccg cccggtgggg caccccggcg
     5221 tccgggttgg aggtgctgcg cgccgcggag tacacgttcc gggcaaggat caccgaccgg
     5281 tggcgccatg gccgggtgct gctgctcggc gacgccgccc acctgacgcc cccgttcatc
     5341 ggtcagggcc tcggcgccgg gctgcgcgac gcccacaacc tcgcctggaa gctcgccgcc
     5401 gtgctgggcg gcgccgacga ggacgtcctc ctcgacagct accaggccga gcgcgcgccg
     5461 catgcggagg cggtgatccg cggcgccgtc ctcgtgggac gggcgatgac cggcggacag
     5521 gacatcgctg ccgcgttgcg ccgcccggcg accggcctgc tcctccgcgt accgggggtg
     5581 cgtgcggcgg cgcagcgcgg gatcgcgacg cggtatgcgc ctggaccgct ggtcgaccgc
     5641 cgccgacacc gccgcgacct cgccggcaca cactgcccgc agccgaccgt cctggtcgac
     5701 gggcacccgt cccggctcga cgacgtcctg ggcgacggct acaccctcct cgccgcgggt
     5761 cccgtgcccc ctgcgctgcg ggccagggcg cagcgactcg gcgccacggt cctccaactc
     5821 cgcccgaccc cggaagcggc cggtcgagtg cagcacgacc atgggcagct ggacgacggc
     5881 gggcagctgc gcgcgtggct tgcccgcggc cgcgcaggtg cggccctgct ccgccccgac
     5941 cggatcgtcc tggccaccgc cccacacgca ccctgacccc gcgagtcgcc cgttccccgc
     6001 ccgcgagtcg cccggttccc gcccgcgagt cgcccgtttc gcgaccccgt gtcgcccgtt
     6061 tcgcgacccc cgtgacgtcc cgtttcgcgc gccggcgagg gcagtttccc gcccgcgagt
     6121 cgcctcttgc tgccgtgact cgcccgcggg ggcgcgagtc gtcacgattg cgggccacga
     6181 acgggcggac ccaacgtgcc cgatgcgggc ggccgagccc gtagccacgc cgccactgac
     6241 ggatcttccg taggcggccc tcatcatgga caccatcccg cagggtgact cgcccgccgt
     6301 ttggggcgac tcgggggcgg tttcggggcg actcgcgggc ggttttcggg cgactcgcgg
     6361 gcggtttctg agctaggtgc cgcgcagcag ggcgacgtag agcgtgagcc cgggcccgaa
     6421 ggccattgcc accgtggcgc gtctcgaacg cggacgcctc atggtgcgca gggcgtcgag
     6481 cacgaggagc accgtggccg acgagcagtt gccgcgctcg gcgagcactc ccagcgaggc
     6541 gtccaacgcg tcgtccggta gcccgagccg ctccgcgacg gtgctcagga tccgggggcc
     6601 gccggggtgc acagcccacc cgtccacgtc cgccgtggtc agcccgtgcc gtccgagcag
     6661 ccccgacacc atgtcgctga cgtggcgagc cagcacccgt ggcacctcgg gcgagagccc
     6721 catccggaac cccaggtcgg taacgtccca ggtcatgtgg tcggcactcg ccgcgtcggt
     6781 gaccgccgcg atctcgacca cctcgagtcc accaacgcgc cgagggtccg ctgcgttcgg
     6841 caacacgacg acagcagcgg cggcgtcgcc gaacagtgcg tgggacacga cctggcccct
     6901 gtcgacgtga ccgtggtcgt ccgcggccgg ctgcacgtgc agcgacgtca actccaggca
     6961 cagcaccacg gcgggccggc cgcgggcgac gacgtagtcg gcggcagcac cgaggccggg
     7021 gatggcggcg tagcagccca tgtggcccac gaacagccgc tgcacgtcgg aggccatccc
     7081 gagatcggcc gcgacgcgga tatcgaggcc cggcgtgacg tatccggtgc acgagacgac
     7141 cgcgagcagc cccacatcac cgggcgcgag gccggcgtcg gcaagcgccg ccgccaccgc
     7201 ctccttgccc agcgggagcg cctcggcgac gtagcgccgc atccgctcgc cggtcgacca
     7261 acgcgagacg tcctcgacca ccgggttcac cgcggcgtgc ctgcgccgca cccctgctcc
     7321 acggaagatc cgctccgcca ttcggtcacc ggcgaaatgc tgcgcgaaga acccgtccca
     7381 catctcggtc tgggtgagcc cgccccctgg tacggcgatg ccgaatcccg cgatgtacgc
     7441 agaggcgctc acgtggagga atccccgccg ccgcccgatg tcaatcccgg ctcctcctgg
     7501 ccccgccgtc ccacggcctg gaacagcact gcggtggtcg gcaccgacac catcggcacc
     7561 gagtcggccc gtccggttgc ccagcgggcg aggccgacga gcgacggccg gatcccgcgc
     7621 agccgcaggg acaccccgtg ccgggcgcac tcgcgcacga gggcggcacg gtcgacgaac
     7681 agcgcgggat cgtggatgcc cggtggcgcc gcgccgggta tgcgctcggc cagcgtcacg
     7741 gcgacgaacc ggctcagcgc cgtggcagcc agggtgtcga tcacgagcag gccaccaggg
     7801 cgcagcagcc ggcatgcctc cgccacgacg gcggccagat cgggtacatg ctcgaggatc
     7861 tcccccgctg cgacgacgtc ggcgcacccg tcggccagcg gcacggccgc gacgtcagcg
     7921 cgcaccgccg tcaccccgtg ggccgctgcc tgggcgagcg cggacgccgt gacatcgatg
     7981 ccgatgtgcc ggtaacccgc tgcgtcgacg tgcggggcga ggaggccggc tccgcagccc
     8041 aggtcgacca ggacagcctc ctcgcgtggg gccgggggta ccagcgcgcc gcgagccgcc
     8101 gcgagccagt gcagcatcgc gaagggcccc cggggccgcc accagtgccg cgccagggcg
     8161 tcgtactggg ccgggtcatt gcgcgccagc cgggcgattc ggccgttccg gacgtcgggc
     8221 acgggtccag gctgccagac tgcccaccat gcggcctctc accgcgaaag cggtcgcgct
     8281 gctgcgctcc tgtcaccccg aacccacggt cgcagtcacg ctcatggtca cggccctcgc
     8341 ggtgaccacc ggccgggacg tggccggggt gctgctcgtg gccgcggcag tgctcaccgg
     8401 gcagctctcg atcggctggc tcaacgacc
//
LOCUS       DASRSC010000205         2265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4659656,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000205 DASRSC010000000
VERSION     DASRSC010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2265
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4659656"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VFE39_10200"
     CDS             complement(<1..183)
                     /locus_tag="VFE39_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259009.1"
                     /translation="MAQQLFELGRRGLERRELGGELVQRQDVLVADGLVVAHRDVEGA
                     GTGERLVQCRRAQLGVP"
     gene            182..313
                     /locus_tag="VFE39_10205"
     CDS             182..313
                     /locus_tag="VFE39_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259010.1"
                     /translation="MQGDQEAMDGFARAKAGMTSPAEFFSEENVGPIPGPGADGPGR"
     regulatory      411..519
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            673..>2265
                     /locus_tag="VFE39_10210"
     CDS             673..>2265
                     /locus_tag="VFE39_10210"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683603.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase
                     (aminomethyl-transferring)"
                     /protein_id="HET6259011.1"
                     /translation="MTDDRPALHDLERGIPFVERHLGPRAEELDTMLAVIGAESLDDL
                     ADSVVPDSIRDREPISSSLPPAASETAVIAELRALAARNTVTVPMIGLGYSGTITPPV
                     IRRHVLENPAWYTAYTPYQPEISQGRLEALLTFQTTVADLTGLPVAGASLLDEATAAA
                     EAMTLLRRAGRAASPRFVVDADTLPQTIDVLRTRAEPLGIELVVTDLAGGLPDGDLFG
                     LLLSYPGASGAVADPSALIAAAHERGALVAVAADLLALTMLTPPGELGADAAVGTTQR
                     FGVPLGFGGPHAGYLSVQQKHARQLPGRLVGLSRDADGNPALRLALQTREQHIRREKA
                     TSNICTAQVLLAVVAACYAVYHGPDGLRRIAQRAHRMAAVLAAGLRAGGVEVLHEQFF
                     DTVAVRVPGRAVDVADAAHAAGIALRRVDGDTLGIACSEVTTTAHLQAVFAAFGVTAD
                     IAALDDATDDALPAGLVRSSEYLTHPVFHEHRSETSLMRWLRRLADADLALDRTMIPL
                     GSCTMKLNAAVEMEPITWPEFAD"
BASE COUNT          307 a          842 c          807 g          309 t
ORIGIN      
        1 cgggacgccg agctgtgcgc gacggcactg gaccagacgc tctccggtgc ccgccccttc
       61 gacgtcgcga tgcgcgacta ccagtccgtc cgcgacgagc acgtcctgcc gctgtacgag
      121 ttcaccgccg agctcgcgtc gctcgagccc ccgccgcccg agctcgaaca gctgctgggc
      181 catgcagggc gaccaggagg ccatggacgg gttcgcgcgg gccaaggccg ggatgacctc
      241 gcctgcggag ttcttctccg aggagaacgt cggacccatc cccggcccgg gcgcagacgg
      301 tcctggtcgc tgacgccgcg cgcgcccggc cacgaggcgg ccgccgtcgt tcacggccga
      361 gtggggggag ggcgaccgtg ggggagcggt accatatgtc gggtagtcgc cgaacccgcg
      421 cgggagagtc ccggacgccg tcgcagctga cgtcgaccgg gcgccgaagg agcaaatcct
      481 ccccggaatc tctcaggcat ccaggaccgc ccgggtgaga cgcctctgga aagtgacggg
      541 cgtgcccgtc cgccgacggg gaaagcccgc tctgtcacgg ggtgaatctc tcaggcgccc
      601 ggttctcggg cggggacaga gggggagggc agcagttgcg tgccctgccc attgccagac
      661 ctcggaggcc ctgtgaccga cgaccgcccc gcgctgcacg acctcgagcg tggcatcccg
      721 ttcgtcgaac gacacctcgg cccgcgggcc gaggaactgg acaccatgct cgccgtgatc
      781 ggagcggagt ccctcgacga cctcgccgac tcggtggtgc ccgacagcat ccgggaccgt
      841 gagcccatct cctccagcct gccgccggct gcctccgaga cggcggtgat cgccgagctg
      901 cgggccctcg cggcacggaa cacggtcact gtcccgatga tcgggctggg ctactcgggc
      961 acgatcacgc cgccggtgat ccgccggcac gtgctggaga acccggcctg gtacacggcc
     1021 tatacgccct accagccgga gatcagccag ggccggctcg aagcactgct gacgttccag
     1081 accaccgtcg ccgacctcac cggactgccc gtcgccgggg cgtcactgct cgacgaggcc
     1141 accgcggccg ccgaggcgat gacgctgctg cgccgggccg gccgcgccgc gtccccgcgc
     1201 ttcgtggtcg acgccgacac cctgccgcag acgatcgacg tgctgcgcac ccgcgccgag
     1261 ccgctgggga tcgagctggt ggtgacggac ctcgccgggg ggctgcctga cggcgacctg
     1321 ttcgggctgc tgctgtccta cccgggtgcc tccggagcgg tcgccgatcc gtcggcgctg
     1381 atcgctgcgg cgcacgagcg cggtgcgttg gtcgccgtgg ccgcggacct gctggccctc
     1441 acgatgctca ccccgcccgg cgagctcggt gccgacgccg cggtgggcac cacccagcgg
     1501 ttcggggtgc cgctcggctt cggtgggccg cacgcgggct acctctcggt gcagcagaag
     1561 cacgcgcgtc agctgcccgg gcggctcgtc ggcctgtccc gcgacgccga cggcaacccg
     1621 gcgctgcggc tcgcgctgca gacccgagag cagcacatcc ggcgggagaa ggcgacgtcg
     1681 aacatctgca ccgcgcaggt gctgctcgca gtggtcgccg cgtgctacgc ggtgtaccac
     1741 gggccggacg gcctgcgccg catcgcgcag cgcgcgcacc ggatggcagc cgtgctggct
     1801 gccgggttgc gggccggtgg tgtcgaggtg ctccacgaac agttcttcga caccgtggcg
     1861 gtccgcgtgc cgggacgcgc cgtcgacgtg gccgacgccg cgcatgccgc cggcatcgcg
     1921 ctgcgccggg tggacggcga caccctgggc atcgcctgtt ccgaggtgac gaccacggcg
     1981 catctccagg ccgtgttcgc agcgttcggg gtgacggccg acatcgccgc cctcgatgac
     2041 gccacggacg acgcgctccc cgccggcctc gtccggagca gcgagtacct cacccatccg
     2101 gttttccacg agcaccgctc ggagacgtcg ttgatgcgct ggctccgccg cctcgccgac
     2161 gccgacctgg cgctggaccg cacgatgatc ccgctcgggt cgtgcacgat gaagctgaac
     2221 gcggccgtcg agatggagcc gatcacctgg cccgagttcg ccgac
//
LOCUS       DASRSC010000206         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5317962,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000206 DASRSC010000000
VERSION     DASRSC010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5317962"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /gene="sbcD"
                     /locus_tag="VFE39_10215"
     CDS             complement(<1..189)
                     /gene="sbcD"
                     /locus_tag="VFE39_10215"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003102553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HET6259012.1"
                     /translation="MRLLHTSDWHVGKTLKGHSRLDEQEQVLREIVGIAREHEVDAVL
                     VAGDLYDTAAPSAQAQQLV"
     gene            353..1345
                     /locus_tag="VFE39_10220"
     CDS             353..1345
                     /locus_tag="VFE39_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET6259013.1"
                     /translation="MASGLVAALAYAGPPPLTLARTFTSWRFTPGVATTVAALGGGYG
                     IGLRRRPEGAGPWPRGRTVCFGAGVVSIALVGMSFLGVYDDTLFWVRAVQNIVLLMVI
                     PMLLAMGAPITLLRDLLPEPARGRLSRMLHSRAARVATFPLIVTVVLTLPLLVLYLSP
                     LYELTLRNAAASALAGLVLATTGFVYFWTRFRIDPAPRADPYGVTLAITIVEMIGDAV
                     LGVVLWLGPLVAAGFYTALSRDWGPSLRVDQLLGAGVLWIGGDVVGLPFIGVVFARMT
                     REDHQRAAVIDAELDELEAARPASPDSADPGDAAQRPRLWWEDDPELSQRFRQR"
     gene            complement(1376..1843)
                     /locus_tag="VFE39_10225"
     CDS             complement(1376..1843)
                     /locus_tag="VFE39_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259014.1"
                     /translation="MKTVYRVLAYLVALAVVIQAATIAFAVFGLLKWIDDGGVLDRAA
                     TRSGPDSFTGASGFVAHGTNGQMIVPILAVLLLVVSFFAKVQSGVVWAGAVLLTVVVE
                     VVLGTFAHALPELGILHGILAIALFAIAVIAARQAEAAAPREAEPAGTGRPNR"
     gene            complement(1983..>2563)
                     /locus_tag="VFE39_10230"
     CDS             complement(1983..>2563)
                     /locus_tag="VFE39_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6259015.1"
                     /translation="GHQVTVVDLHNLPERAQRFVVGVVLASETARKEAAGPGGLLFTM
                     IDELNKYAPREGSSPIKEVLLDIAERGRSLGIILIGAQQTASEVERRIVSNSSVKVVG
                     RLDPAEAGRPEYGFLPASQRQRATLAKPGTMFVSQPEIPVPLAVEFPFPAWATRLSES
                     AGSPAASTNGAVRRDPFARLPAVPDDEDDVPF"
BASE COUNT          346 a          903 c          908 g          406 t
ORIGIN      
        1 cacgagctgc tgggcctgcg ccgagggcgc cgcggtgtcg tagaggtccc ccgcgacgag
       61 cacagcgtcg acctcgtgct cccgggcgat gccgacgatc tcgcggagca cctgctcctg
      121 ctcgtcgagg cgcgaatggc ccttgagagt cttgccgacg tgccagtcgg acgtatgtag
      181 cagtcgcatc agacggacat cgtaggcgcc gcgatctccc gcatccggga cgcgcgccga
      241 tggtggtccg cgcaggaccc gcggcccctg cgcggaccgg atccggcacg cgaaacgcgt
      301 ccgccttccc gcccgcaccg accggctcgg ctccgcgcgt agcgtcgcgg gcgtggcttc
      361 cggactggtc gcggcgctgg catatgccgg tccgccgccg ttgacactgg cacgcacgtt
      421 cacgtcctgg cggttcacgc ccggcgtggc gacgacagtg gccgcgctcg gcgggggtta
      481 tgggatcggg ctgcgccgtc gtccggaagg ggcgggtccg tggccacgtg ggcggactgt
      541 gtgcttcggg gccggggtcg tctcgatcgc gctggtcggg atgtcgttcc tcggcgtcta
      601 cgacgacacg ctgttctggg tgcgggcggt gcagaacatc gtgttgctga tggtgatccc
      661 gatgctgttg gcgatgggtg ccccgatcac gctgctgcgt gacctgctac ctgagccggc
      721 acgcggccgc ctgtcgcgga tgctgcacag ccgggcggcc cgggtcgcca cctttccgtt
      781 gatcgtcacc gtcgtgctga ccctgcccct gctggtgctg tacctcagtc cgctgtacga
      841 gctgaccctg cgcaacgcgg cagcgagcgc gcttgccggg ctggtcctgg cgaccacggg
      901 gttcgtgtac ttctggaccc ggttccgcat cgatccggcg ccccgggccg atccgtacgg
      961 ggtgacgctc gcgatcacga tcgtggagat gatcggcgac gccgtgctcg gtgtggtgct
     1021 gtggctgggc ccgctggtcg cggccggctt ctacacggcg ctgtcccgcg actggggccc
     1081 gagcctgcgc gtcgaccagc tgctcggagc cggcgtgctc tggatcgggg gcgacgtggt
     1141 ggggctgccc ttcatcggag tcgtgttcgc gcggatgacg cgcgaggatc accagcgcgc
     1201 tgctgtgatc gacgcggagc tcgacgagct cgaggccgcc cggcccgcgt ctcccgattc
     1261 tgccgatccg ggagacgcgg cgcagcgccc gcggctgtgg tgggaggacg acccggagct
     1321 gtcgcagcgg tttcggcaac gctgagcgtg cccccggacg aggcgctcgt gcggctcacc
     1381 ggttcggccg gcctgtgcct gccggctccg cctccctggg ggcggctgcc tccgcctgcc
     1441 gtgccgcgat cacggcgatc gcgaacaacg cgatggcgag gatcccgtgc aggatgccga
     1501 gctccggcag ggcgtgggcg aaggtgccca gcaccacctc gaccacgacc gtgagcagca
     1561 cggcaccggc ccagacgacg ccgctctgca ccttcgcgaa gaaggacacg acgagcagca
     1621 gcacggcgag gatcgggacg atcatctgcc cgttggtccc gtgtgcgacg aagccggagg
     1681 cgccggtgaa cgaatcgggt ccgctcctcg tcgcggccct gtcgagtacc ccgccgtcgt
     1741 cgatccactt cagcaacccg aacaccgcga acgcgatcgt cgcggcctga atgacgaccg
     1801 cgagggccac cagataggcc aggacgcgat agaccgtttt catggatctc cctcgtcaga
     1861 gcacgcttgg tcgtcggctg accggaccgt gctccatcag aggtcagaat cggcgggacg
     1921 gccacccccg acgtccggtc ccgccgttac ctcccgaaac atccgctact gcatgagccg
     1981 actcagaacg ggacgtcgtc ctcgtcgtcg ggcaccgcag gcagccgggc gaagggatcg
     2041 cgccgcacgg caccgttggt gctcgcggcc gggctacccg ccgactcgga cagccgcgtc
     2101 gcccaggccg ggaacgggaa ctcgacggcc agcggcaccg ggatctccgg ctgcgagacg
     2161 aacatcgtgc ccggcttcgc gagcgtcgcg cgctgccgct gggacgcggg caggaagccg
     2221 tactcgggac gccctgcctc ggcggggtcg agcctgccga ccaccttcac cgacgagttc
     2281 gagacgatcc gccgctccac ctcgctcgcc gtctgctgcg caccgatgag gatgatcccc
     2341 agtgagcggc cgcgctcggc gatatcgagc agcacctcct tgatcgggct cgatccctcc
     2401 cgcggcgcgt acttgttgag ctcgtcgatc atcgtgaaca acagcccgcc gggccctgcg
     2461 gcctccttgc gggcagtctc ggatgccagc accacgccga cgacgaaccg ctgggcgcgc
     2521 tcggggaggt tgtgcaggtc gacgaccgtg acctggtgtc cgt
//
LOCUS       DASRSC010000207         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5482965,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000207 DASRSC010000000
VERSION     DASRSC010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5482965"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="VFE39_10235"
     CDS             complement(<1..543)
                     /locus_tag="VFE39_10235"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET6259016.1"
                     /translation="MTRRLLMWDVDRTLLVGGGVGVEAYAAAFTAVTGRAWAGTLIAA
                     GRTDLEITPQLFAQHGIADAAPHLASFFTRYASEFGARSHLVRGRGWLLPGVADVLAA
                     LDARPGVIQTLVTGNITAIGIAKVAAFGLDRYFDTTIGGYGDDDAVRSELVRRSRRRA
                     EAAHGPFGAEDVLVIGDTEHD"
     gene            673..897
                     /locus_tag="VFE39_10240"
     CDS             673..897
                     /locus_tag="VFE39_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259017.1"
                     /translation="MAGLTIFAVLLVALALAAIRYGADTRTADSWTPGRGEPEAPRAR
                     ASVRSDLAALLRAIRGVHIPRRRSAHVSCG"
     gene            965..1918
                     /locus_tag="VFE39_10245"
     CDS             965..1918
                     /locus_tag="VFE39_10245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET6259018.1"
                     /translation="MASIGYFLSCEQFGPKELIEQARRAEEAGFERLWISDHFHPWND
                     EQGQSPFVWGVIGALSEATSLPVTTAVTCPTFRIHPAIIAQAAATAAVQLDGRFVLGV
                     GSGEALNEHVLADHWPSIGIRREMLEEAIEIIRRLHAGKEISHHGTYYEVQEAKIYTR
                     PERPVPIYVSAFGPQSAELAGRVGDGLCTTMPDSDLLQVFREAGGAGKPTQAGTKVCW
                     DRDAERAVETAHRIWGNEGLPGQLAQVLPRPRDFMDVQPLVPRDVIASSMTCGDDADK
                     HIAQVQRYVDAGLDEVYVQQIGHDLPGFFDAWRKDVLPAFG"
BASE COUNT          319 a          725 c          755 g          297 t
ORIGIN      
        1 gtcgtgctcg gtgtccccga tcaccagcac gtcctcggcg ccgaacggcc cgtgggcggc
       61 ctcggcccgg cgcctgctgc gccgcacgag ctcggaccgg acggcgtcgt cgtcgccgta
      121 gccgccgatc gtggtgtcga aataccggtc cagcccgaac gccgccacct tggcgatgcc
      181 gatggcggtg atgttgccgg tgacaagggt ctggatcacg ccgggacggg cgtccagcgc
      241 ggcgaggacg tcggcgacgc cgggcagcag ccacccgcgg ccgcgcacga gatggctgcg
      301 tgcaccgaac tccgacgcat agcgtgtgaa gaagctcgcg aggtgcggag ccgcgtcggc
      361 gatgccgtgc tgtgcgaaca gctgcggggt gatctcgagg tccgtgcgtc cggccgcgat
      421 gagcgtgccg gcccacgccc tgccggtcac ggcggtgaaa gccgccgcat acgcctcgac
      481 ccccaccccg ccgccgacga gcaaggtccg gtcgacgtcc cacatcagca gccgtcgggt
      541 cacccgccga tcctcgcacc gggaatccct caggccggcg ccgcccggac gccgcaggac
      601 ggcgtgaacc tctgatgccg ggcgccgtcc cttaccccga acctggtctc accagcgaac
      661 acaggagtcg acatggctgg attgaccatc ttcgcggtac tgctcgtggc actggcgctc
      721 gccgcgatcc gctacggcgc cgacacccgc accgcggaca gctggacgcc ggggcggggc
      781 gaaccggagg cccctcgcgc acgggcgagc gtccgctccg acctggccgc gctgctgcgg
      841 gcgatccgtg gtgtgcacat ccccaggcgt cgcagcgccc acgtgagctg cgggtgacac
      901 ccggccacct ctcgcggcac atgattgcga atcgggcgac ctggtccggg gtaggtaaac
      961 cggcatggcg agcatcggct acttcctgtc ctgcgaacag ttcggcccca aggagctcat
     1021 cgagcaggcc cgtcgcgccg aggaggcggg gttcgagcgg ctgtggatct ccgaccactt
     1081 ccatccgtgg aacgacgagc agggtcagag cccgttcgtg tggggtgtga tcggggctct
     1141 gtccgaggcc acgtccctcc ccgtcaccac ggcggtgacg tgtccgacgt tccgcatcca
     1201 cccggcgatc atcgcccagg ctgccgcgac tgcggccgtc cagctcgatg gccggttcgt
     1261 gctcggagtg gggagcgggg aggccctcaa cgagcacgta ctggcggacc actggccgtc
     1321 gatcgggatc cggcgcgaga tgctggagga ggcgatcgag atcatccgac ggctccatgc
     1381 gggcaaggag atcagccacc acggcacgta ctacgaggtg caggaggcga agatctacac
     1441 ccggcccgag cgtccggtgc cgatctacgt ctcggcgttc ggtccgcagt ccgcagagct
     1501 ggccgggcgc gtcggcgacg gcctgtgcac gacgatgccc gacagcgacc tgctgcaggt
     1561 gttccgggag gcgggcggcg ccggcaagcc gacgcaggcg ggcacgaagg tgtgctggga
     1621 ccgggacgcc gagcgggcgg tcgagacggc ccaccggatc tggggcaacg agggcctgcc
     1681 ggggcagctc gcccaggtcc tgccgcggcc gcgggacttc atggacgtcc agccgctcgt
     1741 gccgcgtgat gtgatcgcca gctccatgac ctgcggggac gacgcggaca agcacatcgc
     1801 acaggtccag cgttacgtcg acgccggcct cgacgaggta tacgtgcagc agatcggcca
     1861 cgatcttccg ggcttcttcg acgcgtggcg caaggacgtg ctgccggcgt tcgggtgaac
     1921 tccacgacct gacagcgggg gcggtgcagt ggtgagcgtg gtcacctggg actatgtgtg
     1981 acgggtggtg cgttgaacca ccagagaccg cactggccag gtagtactcg tgccgaaggc
     2041 cccgcgggct cgcccgcggg agcgccgtcc gtgctcccgt ttcactgcga cggaag
//
LOCUS       DASRSC010000208        12426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5151769,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000208 DASRSC010000000
VERSION     DASRSC010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12426
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5151769"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2329
                     /locus_tag="VFE39_10250"
     CDS             <1..2329
                     /locus_tag="VFE39_10250"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET6259019.1"
                     /translation="GWSGSEVVGQRVMGPDPTRRTFDAPTAARILDELGAGRSFSGRV
                     PATPVSGRTLYFRAAPGPGSTIVGVLQDVDDTEASDEAFALLDALWETAPVGLAYFDT
                     EVRYRRLNGAVLDIDGGTVDDRLGRTPEEVHGPVGATIAEGLRAVLADGKPRLDVPVQ
                     GRLWHGRGPFQEWRMYLYPVRAPEGPIVGVGVVLVDVTAAERTRRELAESAAQREHAL
                     NRYQSLVAATSAAVWIRGADGSALRDAPGLRAITGQSAAEHAGWGFLDAVHPAHRHAL
                     DVAWRAAATTEPAEVFVHSYRLRTAAGGYRWFRTRAVPVHSGGRVVEWVGTETDIDDG
                     MRARDRLAVLAQATFAMNAALDPDAEGELVALAEAVVPQFADLCRVFLLEPDPLPQAN
                     CAGRDRRPGAVRTAPGGGTVTGRRSVTRSAPGIPPSPSSEERFTFPAEHPISRSVRTA
                     APVLMQVSDETASHWRAPEDQASWGTVIDVNSVLVAPVFSRGAVVAALLFIACGDRAP
                     YTQDDLALVGDLAARASAAVETAVTFQRSREVSVALQSAMLSLPPRYPGVEIEARYLP
                     AAAELQVGGDWYDAFTLPGGDLAVGVGDVEGHDLPAAATMGQLRSMLRGLAHDSEDPP
                     SAVLARLDRVASGLDVTRFTTLVYGRLSLAGGRAMFRWSNAGHPGPILIGPDGRPRLL
                     SGEVDVVLGVDPGARRHDCEVEIEPGSTLLLYTDGLFERRRDPGDHANTQLLDLVRGG
                     APLPLPEFCDHLVRATIADTGDDIVVLALRLAAGG"
     gene            2326..2982
                     /locus_tag="VFE39_10255"
     CDS             2326..2982
                     /locus_tag="VFE39_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HET6259020.1"
                     /translation="MTIEELVARAAALAAGPRRAILGITGSPGAGKSTLVAALLARLG
                     DVCVAHVPMDGFHLADVALESIGRRDAKGAPDTFDVGGYVALLRRLRAAAADPAEDVV
                     YAPAFERDLEQPIAGAIAVPREARLVLTEGNYLLLDEGRWAEVRPELDEVWFCDPRPD
                     VRLAQLTARHVAFGKEPARAAAWVAAVDEPNAAMVERTRDRAHLIVPAVLLQSLPAAD
                     "
     gene            complement(3000..4154)
                     /locus_tag="VFE39_10260"
     CDS             complement(3000..4154)
                     /locus_tag="VFE39_10260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3500 domain-containing protein"
                     /protein_id="HET6259021.1"
                     /translation="MEQAVTEAPREVADRMAEAAGAWLACLEAEQRQVASGATPGADD
                     PSDAERLRWFYTPTDHGGLTLHQQRPAQQRLAMRLVATGLSTAAYVTAATVMGLENVL
                     DHVEGFTVTFGRERGRDPGMYYLRVFGEPGGAAPWGWRFGGHHVSLNNLVVDGRVAAT
                     TPCFLGSDPATAPLLGGTVLRPLAAPEDLAREIVRSLPPELAQRAVLLPHAPTDIVGG
                     NRSRLADGDEAIPLVGVWRGPFPDPGEQDALKAGSDRAEAATGVDRDGHRALALTGTP
                     KGLPAAELDAGQRELLLQLLATYLGRVPDGVSPLHHYHDPAALDAVHLAWAGSTAAGE
                     PHYYRLQGPRLLAEWDNTQRQVNHAHSVWRDPESDFGFDVLAAHRAAHHS"
     gene            complement(4522..5511)
                     /locus_tag="VFE39_10265"
     CDS             complement(4522..5511)
                     /locus_tag="VFE39_10265"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6259022.1"
                     /translation="MVPLSVLDLVPIGSGSTATVALQHSTALIRRVEELGFRRYWVAE
                     HHGMPGIASSSPAVLIAHLAGATSLIRVGSGGVMLPNHQPLVIAEQFGTLDALHPGRI
                     DLGIGRAPGTDQRTARALRRGAEQVGADDFPEQLTELAAYFRGEGPVVAVPAHGQEPA
                     MWLLGSSGYSAQLAGLLGLPFAFAHHFSAENTLPALALYRESFRPSSILSEPYSMIAA
                     SVLAADTNAQAQRLALPSALQFLRLRLGRPGLVPNLEEASAYPYTAEERAFVDDRMAG
                     QIIGSPDTVREGVHGLVERTGVDELMVLTSTHDGADRLRSYELLAEAVAPARV"
     gene            5634..6569
                     /locus_tag="VFE39_10270"
     CDS             5634..6569
                     /locus_tag="VFE39_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259023.1"
                     /translation="MRPPADPYETGPLEIDQLGAVLAGMLRTPGVQAARAVDGRRGLV
                     VAAVGATGGDDVAALVQLARATSSVAPRGDGLDDVVVTTRRSVHVLRECGVPGTFLHL
                     RLDRERGDVGAARRALRAAGLQRAVRAWLDGPPARPAAPLAPGVFQAGVFQVVSSRPV
                     ASDATTAQPITAQPTTAQPRPAEHGPALPVMVEPVPAPGMPVSPGPRATAVPTSGPSL
                     IPRPRHPTVPAFDQQPALVTLAGVRPQASTGALAVLALQDVDPRADADLPLPRRKPSP
                     RPVTVGPAGDAVLKQAWARDMDALQRVLAGLRRLP"
     gene            6656..8731
                     /gene="thrS"
                     /locus_tag="VFE39_10275"
     CDS             6656..8731
                     /gene="thrS"
                     /locus_tag="VFE39_10275"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET6259024.1"
                     /translation="MPAPASTAVSAPVRVPAGTTAGTAVREAGLPTSGPSTVVVVRDP
                     QGQLRDLAWAPDADVEVEPVGADTEDGRSVIRHSAAHVLAQAVQQLHPEAKLGIGPPI
                     TDGFYYDFDVETPFTPEDLGKLESAMKKIIKAGQRFSRRRFDSLDDARKELADEPYKL
                     ELIDLKGGSEAEDGAVADSEVMEVDASGELTIYDNVHAHTGETVWSDLCRGPHIPTTR
                     FIPAFKLTRSAAAYWRGSEKNPQLQRIYGTAWESQEALDAHLERLAEAERRDHRRLGA
                     ELDLFSFPDELGSGLPVFHPKGGIIRRELEDYSRRRHEEAGYEFVYTPHITKGRLYEI
                     SGHLDWYADSMYPAMHLDEERGPDGEVRKPGQDYYLKPMNCPMHILIFDSRGRSYREL
                     PLREFEFGSVYRYEKSGVIHGLTRVRGMTQDDAHIFCTAEQVQDELKSLLKFVLDLLR
                     DYGLDDFYLELSTRNPDKSVGTDEMWDEATEALREAAEDSGLELVADPGGAAFYGPKI
                     SVQARDAIGRTWQMSTIQLDLNLPERFGLEYTAADGSRQRPVMIHRALFGSIERFFGV
                     LTEHYAGAFPAWLAPVQVVAIPVTDEQVGYVEDVAAQLRRRGVRVEVDAGDDRMQKKI
                     RTHTLQKVPFLMLVGARDVEAGAVSFRFRDGTQLNGMPVADAVAAVADWIASRNNTSP
                     TGDVFAAAP"
     gene            8874..9476
                     /locus_tag="VFE39_10280"
     CDS             8874..9476
                     /locus_tag="VFE39_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET6259025.1"
                     /translation="MSDAAPPGATSGPAEPGAEPRDGVGTPDGFRRLWTPHRLAYIKD
                     AGGDGCPFCRIPGLPDEEGLVVARGRTVYAVLNLHPYNPGHLMVLPYRHVAELEDLTP
                     EEAVELMTCTQAAVRALKEVAAPHAFNVGLNLGTVAGGSLAEHLHQHVVPRWGGDANF
                     IAVVGQTKVIPQLLSETRALLAAAWSVGGEPPEKAGADPD"
     gene            9544..10197
                     /locus_tag="VFE39_10285"
     CDS             9544..10197
                     /locus_tag="VFE39_10285"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HET6259026.1"
                     /translation="MLNVLARVRVNRVTDPIGRWLVGHGVAPDVVTVIGTVGTVAAAL
                     WFLPRNQLFVGTLVITLFVLFDLVDGAMARARGHGTRFGAVLDSTCDRVADGAVFAAL
                     TWWCLGVGDDRALGVAALVCLVAGQLVSYIKARAEGAGLSADGGLVERAERLIVALVG
                     TGLHGLGVPYALPVALWVLAAATVWTVGQRILAVYRSAQAHAGNSVASDSADPAPSP"
     gene            10257..11246
                     /locus_tag="VFE39_10290"
     CDS             10257..11246
                     /locus_tag="VFE39_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol mannoside acyltransferase"
                     /protein_id="HET6259027.1"
                     /translation="MSPPGGTAPGGAPRAARFAERITDAEYAAGWRLVQMLPQSPALG
                     AFRLGADLAARRGGASVRRLRANLARVVPSASPAELDALVREGMRSYARYWWEMFRLP
                     AMDPAEIHARTDPFVIGSGPFFEALDAGRPVVFAVPHSGNWDTAGVWLVEMLRRRGLP
                     PAFTTVAQRLRPESLYRRFVAYRESLGFEVVAAEDGSAAYRALIRRLRAGGVVCLVAD
                     RDVTATGVEVQFLGEPARLPAGPARLAGLTGALLVPAYPRFTPDAWLLRMAEPVPVGR
                     GTDAVAKATQDVADTFGRLIAEAPADWHALQPIWTADHTPHGEASAEGSEADR"
     gene            11288..>12426
                     /locus_tag="VFE39_10295"
     CDS             11288..>12426
                     /locus_tag="VFE39_10295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6259028.1"
                     /translation="MRVGIVCPYSLDVSGGVQAHVVGLAGALEDLGHSVSVLAPAALN
                     TPVPAFVTPAGRALGVPYNGSVARVSFGPVTYARARRWLAENSFDVLHLHEPTTFSLS
                     VLALFAAEGPIVATFHTSTERSRALSAFGGMLRPLMEKVTARIAVSPLARRVQVEHLG
                     GDAVEIPNGVDLPAFADGPLLSGYPRQETVGFLGRFDEPRKGMTVLLDALRALAPSRP
                     ALRVLVVGRGDVGALRRAAGTVADRLDVLGPVDDATKAAALRSVDVFCAPNLGGESFG
                     IVLTEAMAAGAPVLASDLDAFRAVLGGAGAGVSFPRGDAAALAVSLSALLDDPARRAA
                     MSAAGRLRAADFGWPAVAASVVRVYRAAVAADPRRVVVGPGIAGS"
BASE COUNT         1582 a         4445 c         4676 g         1723 t
ORIGIN      
        1 gggctggtcc ggcagtgagg tggtcggaca gcgcgtgatg ggccccgacc ccacccggcg
       61 cacgttcgac gcaccgaccg ctgcccgcat cctcgacgag ctcggcgcgg ggcggtcgtt
      121 cagcggcagg gtgcccgcca cgcccgtcag cgggcgcacg ctctacttcc gtgccgcacc
      181 tggacccggc tcgaccattg tcggagtgct gcaggacgtc gacgacacgg aggcgtcgga
      241 cgaggcgttc gcgctgctcg acgcgctgtg ggagaccgcc cccgtcgggc tcgcgtactt
      301 cgacaccgag gtgcggtacc ggcggctcaa cggtgctgtc ctcgacatcg acggcggcac
      361 ggtcgacgac cggctgggcc ggacgccgga ggaggtccac ggacccgtcg gcgccacgat
      421 cgccgagggg ttgcgcgcag tactcgccga cggcaagcca cggttggacg tcccggtaca
      481 gggacggttg tggcacgggc gtggtccgtt ccaggagtgg cggatgtacc tctacccggt
      541 gcgtgcgccg gaggggccca tcgtcggggt cggcgtggtg ctggtggacg tcaccgcggc
      601 cgagcgcacc cggcgggagc tggcagagtc cgcggcgcag cgcgagcacg cgctcaaccg
      661 gtaccagagc ctggtggcgg ccacgagcgc ggcggtctgg atccgggggg ccgacggcag
      721 cgcgctgcgc gacgccccgg gcctgcgggc gatcaccgga cagagcgccg cggagcacgc
      781 cggatggggc ttcctcgacg ccgtccatcc cgcgcaccgc cacgccctcg acgtggcctg
      841 gcgggcggcg gccaccactg aacccgccga ggtgttcgtc cactcctacc ggctgcgcac
      901 ggccgcgggg ggttaccgct ggttccgcac gcgcgcggtc cccgtccact ccggagggcg
      961 ggtcgtggag tgggtgggca ctgagaccga catcgacgac gggatgcgcg cccgcgaccg
     1021 gctcgccgta ctggcccagg ccacgttcgc gatgaacgcc gcgctcgacc ccgacgccga
     1081 gggcgagctg gtggcgctgg ccgaggcggt ggtcccgcag ttcgccgacc tgtgccgggt
     1141 cttcctactc gagccggacc cgttgcccca ggcgaactgc gcgggccgag atcgtcggcc
     1201 cggcgcagtg cgcaccgccc ccggcggcgg gaccgtcacg gggcggcggt ccgtcacgcg
     1261 gagcgcgccg gggatcccgc cgtcaccgtc gagcgaggaa cgcttcacgt ttcctgccga
     1321 gcatccgatc tctcgcagtg tgcgcaccgc agcgcccgtc ctgatgcagg tgtccgacga
     1381 gacggcgtcg cactggcggg ctccggagga tcaggcctcg tggggaacgg tgatcgacgt
     1441 caactcagtg ctggtggccc cggtgttctc gcgcggcgcg gtcgtggcgg cgctgctgtt
     1501 catcgcgtgc ggggaccggg cgccctacac gcaggacgac cttgcgctcg tcggcgacct
     1561 cgcggcgcgc gcctcggccg ccgtggagac cgcggtgacg ttccagcgga gccgcgaggt
     1621 ctcggtggcg ctgcagagcg cgatgctcag cctgccgccg cggtaccccg gtgtggagat
     1681 cgaggcccgg tacctgcccg cggcggcgga gctgcaggtc ggcggcgact ggtatgacgc
     1741 cttcacactg cccgggggcg acctggcggt cggggtcggc gacgtcgagg gtcacgacct
     1801 gcccgccgcg gccacgatgg ggcagctgcg cagcatgctc cggggactgg cgcacgacag
     1861 cgaggacccg ccgtcggcgg tgctggcccg gctggaccgg gtggcgtccg gactcgacgt
     1921 cacacggttc accacgctcg tctacgggag gctctccctg gccgggggac gtgcgatgtt
     1981 ccgctggtcc aatgccggcc atcccgggcc gatcctgatc ggcccggatg ggcggccgcg
     2041 gctgctgtcg ggtgaggtcg acgtcgtact gggggtcgac cccggggcgc gccggcacga
     2101 ctgcgaggtg gagatcgagc ccggttcgac gctgctgctc tacaccgacg gactgttcga
     2161 gcgccgccgc gaccccggcg accacgcgaa cacgcagctc ctggacctcg tgcgcggggg
     2221 cgcccccctc ccgcttcccg agttctgtga ccacctcgtg cgagcaacca tcgccgacac
     2281 cggcgacgac atcgtggtgc tcgccctgcg tcttgcggcg ggcggatgac catcgaggag
     2341 ctcgtggcgc gggccgctgc actcgccgcc ggcccccggc gagcgatcct ggggatcacc
     2401 ggcagccccg gtgcgggcaa gtcgacgctg gtggcggccc tgctcgcgcg gctgggcgac
     2461 gtctgcgtcg cgcacgtgcc gatggacggg ttccatctcg ccgacgtggc actcgagtcg
     2521 atcgggcgcc gggacgcgaa gggagccccg gacacgttcg acgtcggcgg ctacgtcgcc
     2581 ctgctgcgca ggctgcgggc cgccgccgcc gacccggcag aggacgtggt ctacgcgccc
     2641 gcgttcgagc gcgatctcga gcagcccatc gccggagcca tcgccgtgcc gagagaggcg
     2701 cggctggtgc tcaccgaggg caactacctg ctgctcgacg aggggcggtg ggcggaggtc
     2761 cgccccgagc tcgacgaggt gtggttctgc gacccgcggc cggacgtccg gctggcccag
     2821 ctcaccgccc ggcacgtcgc gttcggcaag gaaccggcgc gcgccgcggc gtgggtggca
     2881 gcggtggatg agcccaacgc cgccatggtg gagcggacca gggaccgggc gcacctcatc
     2941 gtcccggccg tcctcctgca gtcgctcccg gccgccgact gagcacgtcg ccgaatctgt
     3001 cagctgtggt gcgcggcccg gtgcgcggcg aggacgtcga acccgaagtc ggactccggg
     3061 tcacgccaca ccgagtgggc gtggttgacc tggcgctgcg tgttgtccca ctcggccagc
     3121 agccggggcc cctgcaggcg gtagtagtgc ggctcacccg cggcggtcga gcccgcccag
     3181 gcgaggtgga cggcgtcgag cgctgcgggg tcgtggtagt ggtgcagcgg cgatacgccg
     3241 tccgggacgc ggccgaggta ggtggcgagc agttgcagta ggagctcgcg ctgcccggcg
     3301 tcgagctccg cggccggcag gcccttcggc gtgccggtca gcgccagtgc gcggtggccg
     3361 tcgcggtcga caccggtggc ggcctcggcc cggtcgctgc ccgccttcag ggcgtcctgc
     3421 tcgcccgggt cggggaacgg gccgcgccag acgccgacga gcgggatggc ctcgtcgccg
     3481 tcggccaggc gggaccggtt tcccccgacg atgtcggtcg gggcgtgggg tagcagcacg
     3541 gcgcgctggg cgagctcggg cggtagcgag cggacgatct cgcgggcgag gtcctccggt
     3601 gcggcgagcg ggcgcagcac cgtcccgccc agcaacggcg ccgtggccgg gtcggagccc
     3661 aggaagcacg gcgtcgtggc ggcgacgcgg ccatcgacga cgaggttgtt gagcgacaca
     3721 tggtgcccgc cgaagcgcca accccacggt gccgcgccgc ccggttcgcc gaacacccgc
     3781 aggtagtaca tgccggggtc acgcccgcgc tcgcgcccga acgtcacggt gaagccctcg
     3841 acgtggtcga gcacgttctc cagccccatg acggtcgccg ccgtcacgta cgcggcggtc
     3901 gacaggccgg tcgcgaccag ccgcatggca agccgctgct gtgcggggcg ctgctggtgg
     3961 agggtgagtc cgccgtggtc ggtgggggtg tagaaccagc ggagccgctc ggcgtcggac
     4021 gggtcgtcgg cgcccggggt ggcgccgctg gccacctgtc gttgctcggc ctcgaggcac
     4081 gcgagccacg cgcccgccgc ttcggccatc cggtccgcga cctcgcgggg cgcttcggtc
     4141 actgcctgct ccatgcccga gacagtacga cgggatcagc gagcacgtcc gtgatctcgt
     4201 cgtggagcgc ttccgcgccc ggtcaggcaa gcggcgccgg tccgccggac ccgtgggcgc
     4261 ggcgagggcg gatctcgcac cggacggcgg cagcgtcaag cccggtgaga tcggcaatga
     4321 gcgagcttgc gagcgaatca gtgttacagc gcggcacgta cagcaccagg ccccgctcgg
     4381 agagccagac caccacctgg aacgtgcgct cgccccccgc ggccacgccg gccggcgtag
     4441 gccctggggc gggcggggcg tcagtcgcag gcggtcgccg agcagtcagc gatcggggta
     4501 ggtggcgtgg cacagggggg ttcagacgcg cgcaggagcc accgcttccg cgagcagctc
     4561 gtacgagcgc agccggtcgg ccccgtcgtg ggtgctggtg agaaccatca gctcgtctac
     4621 accggtgcgc tccacgagcc cgtggacgcc ctcgcgcacc gtgtcgggcg agccgatgat
     4681 ctggccggcc atccgatcgt cgacgaacgc ccgctcctcc gccgtgtacg ggtacgcgct
     4741 cgcctcttcc aaattcggaa ccaggccggg tcgcccgagt cgcagccgca ggaactgcaa
     4801 cgcgctcggc agggcaaggc gctgcgcctg ggcgttcgtg tcagcggcca gcaccgaggc
     4861 ggcgatcatg gagtagggct ccgacaggat ggacgacggc cggaacgact cgcggtagag
     4921 cgccagtgcc gggagggtgt tctcggcgct gaaatggtgg gcgaacgcga acggcaggcc
     4981 caacagtccg gccagctgcg cgctgtagcc gctggacccg agcagccaca tcgccggctc
     5041 ctggccgtgc gccggcacgg ctaccactgg gccctcgccc cggaagtacg ccgccagctc
     5101 cgtgagctgc tcggggaagt cgtcggcgcc cacctgctcg gcgccgcggc gcagggcgcg
     5161 ggcggtgcgc tggtcggtgc ccggggcacg gccgatcccg aggtcgatcc ggccggggtg
     5221 cagcgcgtcg agtgtgccga actgctccgc gatgacgagc ggctggtggt tgggcagcat
     5281 gaccccgccg gacccgacgc ggatcagcga ggtggccccg gcgaggtggg cgatcagcac
     5341 ggcgggcgac gagcttgcga tccccggcat cccgtggtgc tcggcgaccc agtagcgacg
     5401 gaagccgagc tcctcgacgc gccggatcag cgccgtgctg tgctggagcg ctaccgtggc
     5461 cgtcgagccg gagccgatgg ggacgaggtc gagcactgag agaggcacca catttgatcc
     5521 aaccgcgacg cgcggacggt tcttcccggg cgttccgacg ccgaatggcc catgacgggc
     5581 gctgccgagc gggtgccctg ccgctgctcc ggaccgtcac gccaggctga ggtatgcgcc
     5641 cccccgccga cccgtacgag accggtccgc tcgagatcga ccagctcggc gccgtgctcg
     5701 ccggcatgct gaggacgccc ggcgtccagg ctgcccgcgc ggtggacggc cggcgggggc
     5761 tggtcgtcgc cgcggtgggc gcgacgggcg gagacgacgt ggccgccctc gtccaactcg
     5821 cccgcgccac ctcttccgtc gcgccgcggg gggacgggct cgacgacgtg gtcgtcacca
     5881 cccgccggtc ggttcacgtg ctgcgggagt gtggggtgcc cggaacgttc ctgcacctcc
     5941 gcctcgaccg ggagcgcggg gacgtcggcg ccgcccgccg ggccctccgt gcggccggcc
     6001 tgcaacgggc ggtgcgggcc tggctggacg gtccaccggc ccggccggcg gcgccgctgg
     6061 cgccgggcgt cttccaggcg ggcgtcttcc aggttgtctc ctcgcggccg gtcgcctccg
     6121 atgcgacaac tgcccagccg atcacagctc agccgacaac tgcccagcca cgtcctgcag
     6181 agcatgggcc ggcgctaccg gtgatggtgg aaccggtgcc cgcgccgggg atgccggtct
     6241 caccgggccc tcgggcgacg gccgtaccga cctccgggcc gtcgctcata ccgcggccgc
     6301 ggcacccgac cgtgcccgct ttcgaccagc agcccgcact ggtgaccctc gccggggtgc
     6361 ggccgcaggc cagtaccggg gccctggccg tgctggcgct gcaggacgtc gacccccggg
     6421 ccgatgccga tctgccgctg ccgcgccgca agccgtcccc ccggccggtg acggtgggcc
     6481 cggcggggga tgccgtcctc aagcaggcat gggcccggga catggacgcg ttgcagcggg
     6541 tgctggccgg gttgcgcagg cttccgtaga agccgccggc gggcgctgcc ggaaaccccc
     6601 tcgccgtgcc cctctagcat cgacaccaca gccgtctact gatggaggcc ctctcgtgcc
     6661 cgcgccagca tccaccgccg tgtccgctcc cgtccgggtg ccggcgggga cgacggcggg
     6721 caccgccgtg cgcgaggcgg gcctccccac gagcggcccg agcaccgtgg tcgtcgtccg
     6781 cgacccgcag gggcagctcc gcgacctcgc ctgggcgccc gacgccgacg tcgaggtcga
     6841 gccggtcggc gccgacaccg aggacggccg cagcgtcatc cggcactccg cggcgcacgt
     6901 gctcgcacag gccgtgcagc agctgcatcc cgaggcgaag ctcgggatcg ggccgccgat
     6961 caccgacggc ttctactacg acttcgacgt cgagaccccc ttcacccccg aagacctcgg
     7021 caagctcgag tcggcgatga agaagatcat caaagccggc cagcgcttct cccggcgccg
     7081 cttcgactcc ctggacgacg cccgcaagga gctcgccgac gagccgtaca agctggagct
     7141 gatcgacctc aagggcgggt cggaggccga ggacggggcg gtcgcggaca gcgaggtcat
     7201 ggaggtcgac gcctccggcg agctgaccat ctacgacaac gtgcacgccc acaccgggga
     7261 gacggtgtgg tccgatctct gccgcggccc gcacatcccc accacacggt tcatccccgc
     7321 gttcaagctc acgcgctccg ccgccgcgta ctggcgcggc agcgagaaga acccgcagct
     7381 gcagcgcatc tacggcacgg cgtgggagag ccaggaggcc ctcgacgccc acctggagcg
     7441 gctggccgag gcggagcgcc gcgaccaccg ccgcctcggt gcggagctcg acctgttctc
     7501 gttccccgac gagctgggct cgggcctgcc ggtgttccac ccgaagggcg ggatcatccg
     7561 gcgcgagctg gaggactact cccggcgccg gcacgaggaa gcgggctacg agttcgtcta
     7621 cacgccgcac atcaccaagg ggcggctcta cgagatctcc gggcaccttg actggtacgc
     7681 cgacagcatg tacccggcga tgcacctcga tgaggagcgc ggcccggacg gtgaggtgcg
     7741 caagccgggg caggactact acctcaagcc gatgaactgc ccgatgcaca ttctgatctt
     7801 cgactcgcgc gggcggtcgt accgcgagct gccgctgcgc gagttcgagt tcggctcggt
     7861 gtaccgctac gagaagtcgg gcgtgatcca cgggctcacc cgcgtgcgcg gcatgaccca
     7921 ggacgacgcc cacatcttct gcaccgcgga gcaggtgcag gacgagctca agtcgctgct
     7981 caagttcgtg ctcgacctcc tgcgcgacta cggcctcgac gacttctacc tcgagctgtc
     8041 gacgcgcaac cccgacaagt cggtcggcac ggacgagatg tgggacgagg ccaccgaggc
     8101 gctgcgcgag gccgccgagg actcgggcct cgagctcgtc gccgacccgg gcggcgcggc
     8161 gttctacggg ccgaagatct cggtgcaggc gcgggacgcc attggccgta cctggcagat
     8221 gtcgaccatc cagctcgacc tcaacctgcc cgagcgcttc ggcctcgagt acaccgccgc
     8281 ggacggctcg cggcagcgcc ccgtcatgat ccaccgggcc ctgttcggtt ccatcgagcg
     8341 gttcttcggc gtgctcaccg agcactacgc cggtgccttc cctgcgtggc tcgcccccgt
     8401 gcaggtcgtc gcgatcccgg tcaccgacga gcaggtcggg tacgtcgagg atgtcgccgc
     8461 gcagctgcgc cggcgcggcg tccgggtcga ggtggacgcg ggtgacgacc ggatgcagaa
     8521 gaagatccgg acccacacgc tgcagaaggt gccgttcctg atgctcgtgg gtgcgcggga
     8581 tgtcgaggcg ggtgctgtga gcttccggtt ccgcgacggc acgcagctca acgggatgcc
     8641 cgtagccgac gcggtggcgg cggtcgccga ttggatcgcg agccggaaca acacgtcccc
     8701 gaccggcgat gtcttcgcgg ccgcgccgta actgccggtc cgggcgcctc tgacggcacc
     8761 gtcgggtcgt ccgcccaccc gaggtaggag gccgatccgg cgggctgtca ggaaccacct
     8821 ggatcccggc tctcatcgga caagatccat cggacacgcc ttaggatcgc gccatgagcg
     8881 acgcggcccc gccgggagcg acgagcggcc cggccgagcc aggggccgag ccgcgcgacg
     8941 gcgtcggcac gcctgacggc ttccgcaggc tgtggacgcc gcaccggttg gcgtacatca
     9001 aggatgccgg gggcgacggt tgcccgttct gccgtatccc cggcctcccg gacgaggagg
     9061 gtctcgtcgt cgccaggggg cgcacggtct acgcggtcct caacctgcac ccctacaacc
     9121 ccgggcacct catggtgctg ccctaccggc acgtcgccga gctggaggat ctcacccctg
     9181 aggaggcggt cgagctcatg acctgcaccc aggccgccgt ccgggcgctg aaggaggtgg
     9241 cggcgccgca cgcgttcaac gtcgggctga acctgggcac cgtcgcgggt ggctcgctgg
     9301 ccgagcacct gcaccagcac gtcgttccgc gctggggtgg cgacgcgaac ttcatcgcgg
     9361 tcgtcgggca gaccaaggtg attccgcagc tgctctctga gacgcgtgcg ttgctggccg
     9421 ctgcatggtc ggtgggcgga gagccgccgg aaaaggccgg tgccgacccg gactaggctg
     9481 caagtggccc gggcggcggc cggcggccgg ccaccgtggg ccccggtcga caagcaggag
     9541 tccatgctca acgtcctcgc ccgcgtgcgc gtcaaccgcg tgaccgaccc gatcggccgg
     9601 tggctcgtcg ggcacggggt cgcgcccgat gtggtcactg tcatcggcac ggtcggaacg
     9661 gttgccgcgg cgctctggtt cctcccgcgc aaccagctct tcgtcggcac gctggtgatc
     9721 acgctcttcg tgctgttcga cctcgtggac ggcgcgatgg cacgggcgag ggggcacggc
     9781 acccggttcg gggcggtgct cgactccacg tgcgacaggg tggcggacgg cgcggtgttc
     9841 gccgcgctca cctggtggtg cctcggagtg ggcgacgacc gcgccctggg ggtggccgca
     9901 ctggtctgcc tcgtcgcggg ccagctcgtc tcctacatca aggcccgggc agaaggtgcg
     9961 gggctgtcgg cggacggcgg gctcgtcgag cgggccgagc gcctcatcgt cgcgctcgtc
    10021 ggtaccggcc tgcacgggct gggcgtgccc tacgcgctcc ccgtcgccct ctgggtgctg
    10081 gcggcggcga cggtgtggac ggtcgggcaa cggatcctcg cggtgtaccg gagcgcgcag
    10141 gcgcatgcgg ggaattctgt ggcgagcgac tccgccgacc cggcgccgag cccgtgaccg
    10201 cccgactcga gccctcgcac gccgacgggg tgccggaggc ggtgtccgcg cgcaccgtgt
    10261 ccccgcccgg cgggacggcg ccgggtggcg caccacgcgc tgcccggttc gccgagcgga
    10321 tcaccgatgc tgagtacgcg gcggggtggc ggctggtgca gatgctcccg cagagcccgg
    10381 cgctcggggc tttccggttg ggcgccgacc tcgccgcgcg ccgcggcggg gcgagcgtgc
    10441 ggcgactgcg ggccaacctg gcgcgcgtcg tgccgtccgc ctccccggcc gagctcgatg
    10501 cgctggttcg cgagggcatg cgcagctacg cccgctactg gtgggagatg ttccggctgc
    10561 cggcgatgga tccggcggag atccacgccc ggaccgaccc gttcgtcatc ggcagcggcc
    10621 cgttcttcga agcgctcgac gccggccgtc ccgtggtctt cgcggttccg cacagcggca
    10681 actgggacac cgcaggcgtg tggctggtgg agatgctgcg ccggcgcggc ttgccgccgg
    10741 cgttcacgac tgtggcgcag cggctgcgcc cggagtcgct ctaccggcga ttcgtcgcct
    10801 accgggaatc gctcgggttc gaggtcgtcg cggccgaaga cgggtcggcg gcgtaccggg
    10861 cgctcatccg gcgactgcgc gccggtggcg tcgtctgcct cgtggccgac cgggacgtca
    10921 cggccaccgg cgtcgaggtg cagttcctcg gcgagcctgc gcgtcttccc gcgggccccg
    10981 cccggttggc cgggctcacg ggcgccctgc tggtgccggc ctacccccgc ttcaccccgg
    11041 acgcctggtt gctgcggatg gcggagccgg tgccggtggg caggggcacc gacgccgtgg
    11101 caaaggcgac gcaggacgtc gccgacacgt tcggccggtt gatcgccgag gcgccggcgg
    11161 actggcacgc gctgcagccg atctggaccg ccgaccacac tccgcacggc gaagcgtcgg
    11221 cagagggcag cgaggccgat cgatgagtga gcttccgagc gggttatcgc tgaggaggca
    11281 gcaggcgctg cgggtgggca tcgtctgccc gtactccctc gacgtctccg gtggggtgca
    11341 ggctcacgtc gtgggcctcg caggagcgtt ggaagacctc gggcacagcg tcagcgtgtt
    11401 ggctcccgcc gccctcaaca cgccggtacc cgcgttcgtc actccggccg ggcgggcgct
    11461 gggtgtgccc tacaacgggt cggttgcgcg ggtgtcgttc gggccggtca cctacgcgcg
    11521 ggcgcgccgc tggctcgccg agaactcctt cgacgtgctc cacctgcacg agccgaccac
    11581 gttcagcctg tccgtgctgg cgctgttcgc cgccgagggg ccgatcgtgg cgacgttcca
    11641 cacctccacg gagcggtcgc gcgcgctgag tgcgttcggc gggatgctgc ggccgctgat
    11701 ggagaaggtc acggcgcgga tcgcggtgtc gccgctggcc cgccgcgtac aggtcgagca
    11761 tctcggtggc gacgccgtgg agatccccaa tggggtcgac ctcccggcct tcgccgacgg
    11821 gccgctgctg tccggctatc cgcgccagga gaccgtcggc ttcctgggcc ggttcgacga
    11881 gccgcgcaag ggcatgaccg tgctgctcga cgcactccgt gccctggccc cgtcgcgacc
    11941 tgccctgcgt gtgctggtgg tcggcagggg ggatgtgggc gcgctgcgtc gcgccgcggg
    12001 cacggtggcc gaccggctcg atgtgctcgg tcctgtcgac gacgcgacaa aggctgccgc
    12061 gctgcgatcg gtggacgtgt tctgcgcgcc gaacctggga ggcgagagct tcggcatcgt
    12121 actgacggag gcgatggcgg ccggggcgcc ggtgctggcc agcgatctcg acgccttccg
    12181 tgccgtgctg gggggggcgg gggcgggcgt ctccttcccg aggggcgacg ccgctgcgct
    12241 ggccgtgtcg ctctccgccc tgctggacga ccccgcgcgg cgcgccgcga tgtccgcggc
    12301 aggccgactg cgggccgccg acttcggctg gcccgccgtg gccgcctccg tggtgcgggt
    12361 ctaccgggcc gcggtggcgg ccgacccgcg ccgcgtcgtc gtcggtcccg ggatcgcggg
    12421 atcgtg
//
LOCUS       DASRSC010000209         7503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5319080,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000209 DASRSC010000000
VERSION     DASRSC010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7503
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5319080"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VFE39_10300"
     CDS             complement(<1..255)
                     /locus_tag="VFE39_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259029.1"
                     /translation="MASAGVTGGTAGEIGSGGDGRRPAPSTILVYSHRPEVREAIINA
                     VGRRPAPDVGRVTFREAGGVAEVLAACDAGEVDLAILDGEA"
     gene            414..1022
                     /locus_tag="VFE39_10305"
     CDS             414..1022
                     /locus_tag="VFE39_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HET6259030.1"
                     /translation="MTTAAPNISARIHSLNRPNLVSIGTIVWLSSELMFYAGLFAIYF
                     TARAQNPPGQTWPPAPTELDVPYALVVTIVLVASSFTCQFGVFAAERGDVFGLRRWYL
                     LTLLMGTFFVLGQANEYHTLVTEHETTFASGPYGTVFYLATGFHGLHVTGGLIAFIFL
                     IIRTKLSKFTPQQATAAIVVSYYWHFVDVVWIGLFAVIYFIR"
     gene            1053..1895
                     /locus_tag="VFE39_10310"
     CDS             1053..1895
                     /locus_tag="VFE39_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HET6259031.1"
                     /translation="MTTTPPSRRLRGPRSRFRRRLAGALALGIGLLAAGGLYTMLAPQ
                     PQVAIAQPTDAALVAKGQELYNSHCITCHGTNLQGVQNRGPSLIGVGDAAAYFQVSSG
                     RMPLARQEAQAVRKPPLPEFDPSTPEGAANLDALGAFVQANGGGPTLPAESDAALRGN
                     DTARGGELFRLNCASCHNFTGRGGALTSGKFAPSLDPATNTQIYAAMLTGPQNMPRFS
                     DRQLTPEEKTDIIGYIKSVTNGNNNPGGDPLGGIGPVSEGLIAFIVGLAGLIGFALWL
                     GAKS"
     gene            1892..3046
                     /locus_tag="VFE39_10315"
     CDS             1892..3046
                     /locus_tag="VFE39_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HET6259032.1"
                     /translation="MTTSRQDPPALGTEHPHPRIPSAAEVEDMTPEQRAILAAELDDV
                     VIVHNQRKWPIPGTRAEKRAERSVALWFAISALSALAFLLAFLFWPFEYRAPSQPGYT
                     MYALYTPVVGATFGLAVLALGIAVIAYVKKFFPDEVSVQQRHDGASDEIARQTVMAQL
                     AKAGQDTGIARRKLITRAAGAAAGIFGLGLGIAAIAPLVRNPWKAGPNSPLWVTAWKP
                     VAGETVYLRRDTGIPDEISLVRPEDQEAGSMETVFPFRESERGNPEALQAALQGSDTP
                     VMLIRFRPGTQVTQGPGTENYHYGDYYAFSKICTHLGCPASLYEAQTQRILCPCHQSQ
                     FLATDDAKPIFGPATRALPKLPITVNNEGYLVATGDFNQPVGPAFWELRS"
     gene            3043..4767
                     /locus_tag="VFE39_10320"
     CDS             3043..4767
                     /locus_tag="VFE39_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase cytochrome b
                     subunit"
                     /protein_id="HET6259033.1"
                     /translation="MSGITTPTSSSGGLHAKGAAALDELDQRYHPAAGMRKQFNKVFP
                     THWSFMLGEIALYSFIVLLLSGTYLGLFFDPSMVEVTYNGPFDNLRGIHMSRAYESAL
                     EISFQVRGGLFVRQVHHWAALLFVAAMVAHMCRTFFTGAFRKPREANWVIGVLLLFLG
                     FMEGFSGYSLPDDLLSGTGLRIASGFILAVPVIGTWTQWALFGGEFPGTEILPRLYII
                     HVLLIPGILLALIAVHVGLVWYQKHTQFPGPGRTENNVVGVRILPAFAAKGGAFFAVT
                     VGVLGIMGGVFQINPIWNFGPYNPSHISAGSQPDWYVLFTEGMLRIFPPWNIAIGDYT
                     IPPAFWASPAFLPVLYVLAGVYPTIERHFTKDNALHNLLQRPRDVPVRTSLGVMAIAF
                     YGMLVLSGSNDLIAYFFDISLNATTWAGRIGVLVVPPLAYYVSYRICIGLQRSDRAVL
                     DHGIETGIIKRLPHGEFIEVHQPLAGVDDHGHPIPLEYQGAPVPKRMNQLGMGGAPVS
                     GSLLSPDSPEETTALERARAEEAALEAARHNGASTDAGHGEREARTEEREALAGPHAR
                     ESGPHAGD"
     gene            complement(4906..6126)
                     /locus_tag="VFE39_10325"
     CDS             complement(4906..6126)
                     /locus_tag="VFE39_10325"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HET6259034.1"
                     /translation="MVAAQAPDNGMRRRRLMRWGVAAVGVALVGQAVVLALWPAAHLL
                     MIDLQVYRAGGEHIVRHEQLYTGGVLLDLPFVYPPFAAVLFAPLSLLPLDVLKVAWTA
                     GGLGLLAYVVHRCGRSVGVPAGSAATTMLLTLLATCLDPVRTTLYLGQINIVLLAVVV
                     GDVLGRRDSRWRGVGVGIAAAVKLTPLVFVVYLLLTGRKRAAATAVGVFVAATGIGFL
                     AAPADSVAYWFDGTFAAADRISDVAGTANHSLNGLLARLGSHGTVAWLVGAVVLGTVT
                     VGLAVRAHRAEQEVLALTLCGLGSAALAPFAWSHHWVWVVPLVVLLAHRAVAGERAAS
                     AALLGVLVATVAVVTALPGPGVGPIPSTGLISLQPDVYLLLFLAVLTASAVQRRTRTG
                     AARSRNQCTLDRFS"
     gene            6175..7296
                     /locus_tag="VFE39_10330"
     CDS             6175..7296
                     /locus_tag="VFE39_10330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET6259035.1"
                     /translation="MPDGERLPSSSMLALTTIRNDDEIDTTTRRRAMKVDAVLQGSGL
                     AELAAEAQEREAAGYDGLWSFEGPHDPFLPLMPVAEHTARVAVGTAIAVAFARSPMTT
                     AYVAHDLQVHSEGRFLLGLGSQVRPHIERRFAMPWSHPAPRMREYVQALRAIWAAWND
                     GEKLNFQGDFYAHTLMTPFFSPAPSPWGPPKVYLAAVGEHMTRAAGEVCDGLQPHTFT
                     TQRYLREQTIPILEEGLAASGRSLADFSISFSGFVVTGTTEEEMATAARGVRQQIAFY
                     GSTPAYRKVLDLHGWGELGVELNRLSRSSDDDRWQRMGELVEDEVVDAFAVVTEPDRL
                     GPALLERYGSIVDRFAFYAPYPHGDDLFGPAIETLRAGS"
BASE COUNT         1149 a         2744 c         2516 g         1094 t
ORIGIN      
        1 cgcctccccg tccaggatcg cgaggtcgac ctcgcccgcg tcgcacgcgg cgagcacctc
       61 ggccaccccg cccgcctcac ggaacgtcac ccggcccacg tccggcgccg gccgcctgcc
      121 gaccgcgttg atgatcgcct cacggacctc ggggcggtgg ctgtagacca ggatcgtcga
      181 cggcgccgga cgtcgcccgt ccccgccgct accgatctcc cctgccgtgc caccggtcac
      241 acctgcgctc gccacggcgc ggatactagt cgtccggacc ggccgggaac cccggccccg
      301 cggcgtccgt tggcggctgt gacgagggac gcggggccgc cgtccaccga tcgtgatcta
      361 ccgaggggta tcccgacagg ctgtagaagt tcgcgtgggg tcataatgcc ccggtgacga
      421 cagcggcacc caacatcagc gcgcggatcc actcgctgaa ccggcccaac ctggtcagca
      481 tcggcacgat cgtctggctg tccagcgagc tgatgttcta cgccgggctg ttcgcgatct
      541 acttcaccgc gcgggcccag aaccctcccg gccagacgtg gcccccggca ccgaccgagc
      601 tggacgtgcc gtacgcgctc gtcgtgacga tcgtgctcgt ggcctcgtcg ttcacctgcc
      661 agttcggcgt cttcgcggcc gagcgcggcg acgtgttcgg cctgcgccgg tggtacctgc
      721 tcacgctcct gatgggcacg ttcttcgtgc tcgggcaggc gaacgagtac cacacgctgg
      781 tcaccgagca cgagacgacg ttcgcgtccg gcccctacgg cacggtcttc tacctggcca
      841 ccgggttcca cggcctgcac gtcacgggcg ggctcatcgc cttcattttc ttgatcatcc
      901 gcaccaagct cagcaagttc accccgcagc aggccaccgc ggcgatcgtc gtgtcgtact
      961 actggcactt cgtcgacgtg gtgtggatcg gcctgttcgc cgtcatctac ttcatccgct
     1021 gaccgccgac agagggctgg aagacgcaga cgatgaccac gacacccccc tcaaggcgac
     1081 tccggggccc gcgtagccgc ttccgccggc gccttgccgg cgcgttggcg ctcggtatcg
     1141 gcctgctcgc cgccggcggt ctctacacga tgctcgcgcc gcagccgcag gtcgcgatag
     1201 cgcagccgac cgatgccgcg ctggtggcga agggccagga gctttacaac tcccactgca
     1261 tcacctgcca cggcacgaac ctgcagggcg tgcagaaccg cgggccgagc ctgatcggtg
     1321 tcggcgacgc ggccgcctac ttccaggtct cgtcgggtcg catgccgctc gcccggcagg
     1381 aggcgcaggc ggtgcgcaag cccccgctgc cggagttcga tccgagcacc cccgagggtg
     1441 ccgcgaacct cgacgcgctc ggcgccttcg tccaggccaa cggcggcggt cccacactgc
     1501 ctgccgagtc cgacgccgcg ttgcgcggca acgacaccgc ccgcggcggt gagctgttcc
     1561 ggctcaactg cgcgtcgtgc cacaacttca ccggccgcgg tggcgcgctg acctccggca
     1621 agttcgcacc cagcctggat ccggccacca acacccagat ctacgccgcg atgctcacgg
     1681 gtccgcagaa catgccgagg ttctccgacc ggcagctcac gccggaggag aagacggaca
     1741 tcatcggcta catcaagtcg gtgaccaacg gcaacaacaa ccccggcggc gacccgctgg
     1801 gcggcatcgg gccggtctcc gagggcctga tcgcgttcat cgtcggcctg gccggcctga
     1861 tcggattcgc actgtggctg ggagcgaagt catgacgacc tcacgccaag acccgcccgc
     1921 gctgggcacg gagcacccgc acccgcgcat cccctccgcc gccgaggtgg aggacatgac
     1981 gccggagcag cgcgccatcc tggccgccga gctcgacgac gtcgtgatcg tccacaacca
     2041 gcggaagtgg ccgatcccgg gcacccgggc cgagaagcgg gccgaacgct ccgttgcact
     2101 gtggttcgcg atctccgcgc tctccgcgct cgcgttcctg ctggccttcc tgttctggcc
     2161 cttcgagtac cgggcgccct cccagcccgg ctacaccatg tacgcgctct acacgccggt
     2221 cgtcggggca accttcgggc tcgcggtgct ggcgctgggc atcgccgtca tcgcctacgt
     2281 gaagaagttc ttccccgacg aggtgtcggt gcagcagcgc cacgacgggg cgtccgacga
     2341 gatcgcgcgc cagaccgtga tggcgcagct cgccaaggcg ggccaggaca ccgggatcgc
     2401 gcgccgcaag ctgatcacgc gcgcggcggg cgcggcggcc ggcatcttcg gcctcgggct
     2461 cggcatcgcc gcgatcgccc cgctggtgcg caacccgtgg aaggccggac ccaactcccc
     2521 actgtgggtc accgcgtgga agccggtagc cggcgagacg gtgtacctgc gccgcgacac
     2581 cgggatcccg gacgagatct cgctggtgcg ccccgaggac caggaagcgg gctcgatgga
     2641 gaccgtgttc ccgttccggg agtccgagcg aggtaacccg gaggcgctcc aggctgctct
     2701 gcagggctcc gacactccgg tgatgctcat ccggttccgc cccggcaccc aggtcaccca
     2761 gggtcccggc acggagaact accactacgg cgactactac gccttctcga agatctgcac
     2821 gcacctgggc tgcccggcat cgctgtacga ggcgcagacg cagcggatcc tgtgcccctg
     2881 ccaccagtcc cagttcctcg ccacggacga cgcgaagcca attttcggtc ccgcgacgcg
     2941 cgcactaccc aaacttccca ttaccgtgaa caacgagggc tacctcgtcg ccaccggtga
     3001 cttcaaccag ccagtcggtc cggccttctg ggagctgaga tcatgagcgg gatcaccacg
     3061 cccaccagct ccagtggtgg gctccacgcc aagggcgccg cggcactcga cgagctggac
     3121 cagcgctacc acccggcagc gggtatgcgg aagcagttca acaaggtctt cccgacgcac
     3181 tggtcgttca tgctcggcga gatcgcgctc tacagcttca tcgtcctgct gctctcgggc
     3241 acgtacctcg ggctgttctt cgacccgtcc atggtcgagg tcacgtacaa cggcccgttc
     3301 gacaacctgc gtggcatcca catgagccgc gcctacgaga gcgccctcga gatctcgttc
     3361 caggtgcgcg gcggcctctt cgtccggcag gtgcaccact gggccgctct gctgttcgtg
     3421 gccgcgatgg tcgcgcacat gtgccgcacc ttcttcaccg gcgcgttccg caagccgcgc
     3481 gaggccaact gggtcatcgg ggtcctgctg ctgttcctcg ggttcatgga gggcttcagc
     3541 ggctactcgc ttcccgacga cctgctgtcg ggcacggggc tgcggatcgc ctccgggttc
     3601 atcctggcgg tgccggtgat cggcacctgg acccagtggg ccctattcgg cggcgagttc
     3661 cccggcacgg agatcctccc gcggctctac atcatccacg tgctgctcat tccggggatc
     3721 ctgctcgcgc tcatcgcggt gcacgtcggg ctggtctggt accagaagca cacccagttc
     3781 ccgggaccgg gccgcaccga gaacaacgtg gtgggcgtcc ggatcctccc ggcgttcgcg
     3841 gccaagggcg gggccttctt cgcggtgacc gtcggcgtcc tcgggatcat gggcggggtg
     3901 ttccagatca acccgatctg gaacttcggc ccgtacaacc cctcccacat ctccgccggt
     3961 tcccagccgg actggtacgt gctcttcacc gagggaatgc tgcggatctt cccgccgtgg
     4021 aacatcgcca tcggggacta cacgatccca ccggccttct gggccagtcc cgcgttcctg
     4081 ccggtgctct acgtgctcgc cggggtctat ccgacgatcg agcgccactt caccaaggac
     4141 aacgcgctgc acaacctgct gcagcggccc cgggacgtcc cggtccgcac ctcgctcggc
     4201 gtcatggcca tcgcgtttta cgggatgctg gtcctgagcg gcagcaacga cctgatcgcg
     4261 tacttcttcg acatctcgct caacgccacc acctgggcag gccggatcgg cgtcctggtc
     4321 gtcccgccac tggcgtacta cgtgtcctac cggatctgca tcggcctgca gcgctccgac
     4381 cgggcggtcc tcgatcacgg catcgagacc ggcatcatca agcggctgcc ccacggcgag
     4441 ttcatcgagg tgcaccagcc gctcgcgggt gtggacgacc acgggcaccc catcccgctg
     4501 gagtaccagg gcgcaccggt gcccaagcgg atgaaccagc tgggcatggg tggcgccccg
     4561 gtatcgggtt cgttgctcag ccccgactcc cccgaggaga cgacggcgct ggagcgggcc
     4621 agggccgagg aggccgctct cgaggcggca cggcacaatg gcgcctccac cgacgccggg
     4681 cacggcgagc gcgaggcgcg caccgaagag cgcgaagcgc tggccgggcc gcacgcgcgg
     4741 gagtcgggac cgcacgccgg cgactgagag tcgcgccagc acccacccga gagggccccg
     4801 ccgggacacc ggcggggccc tctcgccgtt cgcggcttct gatccaccca cccagcgtgc
     4861 accggttccg gttccgggct gaaccggtgc gcgttcgggg gctgctcagg aaaaccggtc
     4921 gagcgtgcac tggttccggc tcctggctgc accggtgcgc gttcggcgct ggacagcgct
     4981 cgccgtgagc actgcgagga acagcagcag gtagacgtcg ggctgcagcg agatcagtcc
     5041 ggtgctcggg atcggcccga ccccgggccc gggcagggcc gtgaccacag cgaccgtggc
     5101 aacgagcacg ccgaggagcg ccgcgctggc agcacgctcg cccgccaccg ctcggtgggc
     5161 gagcaggacg accaggggca cgacccacac ccagtggtgc gaccaggcga acggcgcaag
     5221 cgctgccgag cccagaccgc acagcgtcag cgcgagcacc tcctgctccg cccggtgcgc
     5281 ccgcacggcc agccccacgg tgacggtgcc gagcaccacg gcgccgacga gccacgccac
     5341 cgtcccgtgg ctgccgagtc gcgcgagcag cccgttcagt gagtggttgg cggtgcccgc
     5401 gacgtcagag atccgatcgg cggcggcgaa cgtcccgtcg aaccagtagg cgacagagtc
     5461 ggcaggggcg gcgaggaacc cgatccccgt ggcagcgacg aacaccccga cggcggtggc
     5521 cgcggcccgc ttgcgcccag tgagcagcag gtagacgacg aacaccagcg gcgtcagctt
     5581 cacggcggcc gcgatgccga cgccgacccc gcgccagcgg ctgtccctgc gtcccagcac
     5641 gtcgcccacc acgacggcga gcagcacgat gttgatctgt ccgaggtaga gcgtggtgcg
     5701 caccgggtcc aggcacgtcg cgaggagtgt gagcagcatc gtcgtggcgg cggatccggc
     5761 cggcacaccg acgctgcggc cgcagcggtg cacgacgtag gcgagcaggc cgaggccgcc
     5821 cgccgtccac gcgaccttga gcacgtcgag gggcagcagg gacagtggcg cgaacagcac
     5881 cgctgcgaac ggcgggtaga cgaacgggag gtccaacagc acgccaccgg tgtacagctg
     5941 ctcgtggcgc acgatgtgct ctccgcccgc ccggtacacc tgcaggtcga tcatcagcag
     6001 gtgcgccgca ggccagagcg cgagcacgac ggcctgcccg acgagcgcca cgccgacggc
     6061 ggccacgccc caacgcatca accggcgcct gcgcatcccg ttgtccggcg cctgcgctgc
     6121 gaccatcagc cctccgcggt tgtcggcgcc cgcggctagc gggagcggga tggcgtgccg
     6181 gatggggagc gcctgccgtc gtcgtccatg ctcgcactca ccacgatccg gaacgacgac
     6241 gagatcgaca cgacgacgag gcggagggcc atgaaggtcg acgcagtgct gcagggctca
     6301 gggctggcgg aactcgccgc cgaggcgcag gagcgggagg ccgccggcta cgacgggctg
     6361 tggagcttcg aggggccgca cgacccgttc ctcccgctga tgccggtggc cgaacacacc
     6421 gcacgggtgg cggtgggcac cgccatcgcg gtcgccttcg cccgcagccc catgaccacc
     6481 gcgtacgtgg cgcacgacct gcaggtgcac tccgagggcc gtttcctgct gggcctgggg
     6541 agccaggtgc ggccgcacat cgagcgccgc ttcgccatgc cgtggagcca ccccgccccg
     6601 cggatgcgcg agtacgtgca ggcactgcgc gcgatctggg cggcctggaa cgatggcgag
     6661 aagctgaact tccagggcga tttctacgcc cacacgctca tgacgccgtt cttctccccg
     6721 gcgccgagcc cctgggggcc gcccaaggtg tacctcgcgg ctgtcggcga gcacatgacg
     6781 cgcgccgcag gcgaggtgtg cgacggcctc cagccgcaca cgttcacgac gcagcgttac
     6841 ctgcgcgagc agaccatccc gatcctggag gagggcctcg ccgcctcggg gcgctcgctc
     6901 gccgacttct cgatcagctt ctcgggcttc gtcgtgaccg ggacgaccga ggaggagatg
     6961 gcgacggccg cgcgcggcgt gcggcagcag atcgcgttct acgggagcac ccctgcttac
     7021 cggaaggtgc tggacctgca cggctggggg gagctcggcg tcgagctcaa ccggctctcg
     7081 cgcagcagcg acgacgaccg ctggcagcgg atgggcgagc tcgtcgagga cgaggtcgtg
     7141 gacgccttcg cggtggtcac tgagccggac cggctgggtc ctgcactgct ggagcggtac
     7201 ggcagcatcg tcgaccggtt cgccttctac gcgccctacc cgcacggcga cgacctgttc
     7261 ggaccagcga tcgagaccct gcgcgcaggg agctgacgcc tggggggcac cgggacggtc
     7321 acgacgtcga gaccggtgat cccggcgaag tcggccgggt tgcacgtatg gacaggcagt
     7381 tcgtttctgc aggaccgact gtcgggccgc ccgctcatcg tccgtcgtcg caaccaaggg
     7441 tccgacggac agctcggcga gagtgatcgt cgagatgagc ggctcgttgg gcaggccctg
     7501 ctc
//
LOCUS       DASRSC010000210         9141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1715102,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000210 DASRSC010000000
VERSION     DASRSC010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9141
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715102"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1582)
                     /locus_tag="VFE39_10335"
     CDS             complement(35..1582)
                     /locus_tag="VFE39_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6259036.1"
                     /translation="MSSPTQRLRRWWASRTLRLRITLVVGVVALAALLALSRLGVGLL
                     YGTMLGAADAELRGEAQGVAAQLAAGTPPADVRQPEIRIVDSAGDPVDGGPDLPLRPK
                     QVRELAAGDALTAGRLTELRRWLAVSTVTPDGSTRLVVVTADIVGGTMLLAHAAVGFV
                     VGALVVGAVVVLAAWVATRAALRPVDRMRTAAAALPPGQRLPVPVPRDELRALAEEIN
                     LLLARRDEAVARLERFTGDAAHELRSPVAAIRAQAEVAVAHPDPELAQESLRSVAEEA
                     ARLSTLLSDLLALARADAGQRPPAQSVDLVAAARAAMGRAAGAEADEPVLQLVAPAPA
                     HAGASPSEVALVLDNLIANARRYARSLVRIGVLPARRSVRLVVEDDGPGIPEADRQRI
                     FDRFLRLSPEAGGGAGLGLALVAALVRGRGGSVTAGATPSGGARIEVRWPAAAVPESG
                     PAPGTGPPPAGSARSARPGAGPPDGRSPPAGSARSARPTAGLPDGGSPSARSAGSTTP
                     EAGPGPE"
     gene            complement(1620..2303)
                     /locus_tag="VFE39_10340"
     CDS             complement(1620..2303)
                     /locus_tag="VFE39_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6259037.1"
                     /translation="MARVLVVDDEPGIRTAVARGLTAEGMEVVALADGPSALNAALTG
                     SFDAVVLDIILPGLSGYRVLEQLRAAGVDTPVLLLSAKDGEFDQADGLDLGADGYLVK
                     PFAFVVLVAQLRALLRRRDAALGRTGQRVRLGSLIVDPVSRSVSWNGAVVELSPREFA
                     LLHALACRPDTAVAKDELLRLVWGDEQAASRNVVEVYVGYVRRKLDAVGAGHVLKTVR
                     GYGYIVESS"
     gene            2379..3458
                     /locus_tag="VFE39_10345"
     CDS             2379..3458
                     /locus_tag="VFE39_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HET6259038.1"
                     /translation="MATGQGDARNRGTRSRGRAGRTVAAGVAVAGAVGLGLLAVPGGA
                     GAAPELPPVSAADLVGSVLAARPGAFGGTVELDNALGLPALPNLPQAANGTSTARVWS
                     DGEGRGRIQVPTKAGERTLVSDGATFWSWNSEDRTVTRAPEHSAQRPEGAATDPTAAA
                     TQALAALQPTSEISVDGTAEVAGRPAYELVLAPVPSERTLLREVRIAVDADKRVPLRL
                     TVLANGSSDPALQLGFTNLTFGAQDPSLFTFTPPPGATVQDEQRPQGGQAGKPEAAGP
                     TVVGKGWDTVIVTKKPPQAPARGAEGDRFGGGADLSALGTPVSGPWGSGRLITTTVAG
                     AIVTDDGRIAAGAVPEQVLAEALSQ"
     gene            3455..4444
                     /locus_tag="VFE39_10350"
     CDS             3455..4444
                     /locus_tag="VFE39_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6259039.1"
                     /translation="MTSSPRVDAAAAPAGAAPVRPVAARAVGLRKTFGRTVAVDGIDL
                     EVPAGSVLGMLGPNGSGKTTLIRMLLGLTRPTAGAAELLGHPIPEESARALPQVGALV
                     EGPGFHPFLSGRENLRRAAATEPLLGTAEIREAVDAALHRVGLGDAADRRFRGYSLGM
                     KQRLGLAAALLLPRSLVVLDEPTNGLDPAGTRDVRQIIAELHAGGATVIVSSHLLAEV
                     EATCTHVAVLQSGSLVAAGELNALLATGTGGLEITTPDVELALRTLRSAGATTYRAER
                     SPAEEQAIVVGDDGPAAPEAIALLVRAGVAVHEARRSRSNLDDLFARLTEESS"
     gene            4441..5301
                     /locus_tag="VFE39_10355"
     CDS             4441..5301
                     /locus_tag="VFE39_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6259040.1"
                     /translation="MTSAVLTGEPLSAPHNAPLGRLLAAELRWVLRRPRTLVMLGLFA
                     LVPVLISIGVAVANRTPGPGAGLIGAIAGNGLVLPVAAMTLALALLLPLAVSMAAADA
                     IAGEAAHGTLRGLLIAPVGRLRLVAMKAFGVLVVATLATLALAVMGVLAGLVVVGGAD
                     GQLVTLSGTAVGLGDALARVALVAVWTVWQLAAVGAVALAISSFTEHPLVVLASVLGG
                     LIVFGVLSAIPALEWLQPYLLTSGWTAGADALRDPLPLDGMAESSLRALCYVAIGGGL
                     TIHRMLRRDA"
     gene            5597..5893
                     /locus_tag="VFE39_10360"
     CDS             5597..5893
                     /locus_tag="VFE39_10360"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HET6259041.1"
                     /translation="MTELLDDAAITAALADLPGWERQGDAIVRTAKLPSFPAAVSVVD
                     RVAEIAEGRDHHPDIDIRWRTLTFRCSTHSAGGITEKDTALAASISEQIDAAGG"
     gene            complement(5900..7096)
                     /locus_tag="VFE39_10365"
     CDS             complement(5900..7096)
                     /locus_tag="VFE39_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259042.1"
                     /translation="MSARTYLLAAGAFAVGTSAYVVSGVLPAVSSELGVSLTAAGQLA
                     TAFALAYAVGAPLIATLAGRWDRRTVLLVALVAAAVGNGLSAVATTYPLLIVGRIIAA
                     IGAAAYTPAATLVATALLPPAQRARAVAIVFGGLTLALAIGVPAGDLLGSSIGYGGVF
                     AAVAVVSALVAAAVPICLPRMAAPPAVPLRERFAVAADRRVLTVLAMTVVGVLAAMVV
                     FIYVTPLLNATAGIHGATIALLLVVYGVGAMVGNTWGGRAADRFGTVPTLYAVLAGIA
                     VTVVTLSLTASTLVGAAMALVLWGIFSWAFNPPLQSLLLELAPTGGLVLSLNASAIYL
                     GTALAGVVGGVVIGMGGLLVLPLVGGGLVLVVIALLLTLQLGRRRVAEAEKVPVPACA
                     GLAAAD"
     gene            7242..7514
                     /locus_tag="VFE39_10370"
     CDS             7242..7514
                     /locus_tag="VFE39_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6259043.1"
                     /translation="MRVEQVLHALGDPVRLMIVQALAAAPEGVACGVIPLPVTKSTRT
                     HHLRTLREAGVISMRTEGTRRMSTLRRDDLDALYPGLLDGVLAAPR"
     gene            7563..8357
                     /gene="ppk2"
                     /locus_tag="VFE39_10375"
     CDS             7563..8357
                     /gene="ppk2"
                     /locus_tag="VFE39_10375"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020639778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HET6259044.1"
                     /translation="MSRLPRKPYEKELLRLQGELVQMQEWVRTSGARLVVVFEGRDAA
                     GKGGAIKRVSEFLNPRICRIVALPAPSERERSQWYFQRYTEHLPAAGEIVLLDRSWYN
                     RAGVERVMGFCTPDEYARFLRQCPVFEELLVEDGILLRKYWFSVSDAEQERRFRGRMS
                     DPMRQWKLSPMDLESITRWEDYSRAKDEMFVHTDTAATPWFVVEAEDKRRARINMIAH
                     LLATVPWQPVASRDLELPQRPPRRGYQRPPRDRFNEVPDHAAMLVG"
     gene            complement(8397..>9141)
                     /locus_tag="VFE39_10380"
     CDS             complement(8397..>9141)
                     /locus_tag="VFE39_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET6259045.1"
                     /translation="HNLGLIEDGARRAAAAGARVVVFPEAAMCCFGVPLGPVAEPLDG
                     PWAGRVRAIADAAGVTVVAGMFTPADDGRVHNTLLATGGGVEAAYDKIHLFDAFGFAE
                     SRTVAPGSKPVSIVVDEVTVGLATCYDLRFPGLFQELAGNGATVVLVPASWGAGPGKR
                     EQWDLLVRARALDSTAFVVACDQADPTTVGRDAGSAPTGIGASLAVGPLGVVLDQLGA
                     EPGLLLVDLAMDEVEQARRAVPVLANRRF"
BASE COUNT         1301 a         3439 c         3327 g         1074 t
ORIGIN      
        1 acgccagggc gctcgcccgc tcctccggcc cgcactactc cggaccgggc ccagcctccg
       61 gagtcgtcga gccggccgac cgggcagacg gagaaccgcc atcgggcaac ccggcggtcg
      121 gcctggcaga gcgggccgac cccgcaggag gagaccggcc atcgggcggt ccagcgcccg
      181 gcctcgccga gcgggccgac cccgcagggg gaggcccggt accgggagcc gggccgctct
      241 cggggacagc cgccgccggc caccgcacct cgatgcgggc cccgccggac ggagtcgcac
      301 cggccgtcac ggagccgcct cggccccgga ccagcgccgc aaccagggcg agcccgagac
      361 cggcaccacc gccggcctcc ggggacaggc gcaggaagcg gtcgaagatc cgctgccggt
      421 cggcctcggg gatgcccgga ccgtcgtcct ccacgacgag ccgcaccgac cggcgcgccg
      481 gcagcacgcc gatccgcacg agggaacggg cataacgacg cgcgttggcg atgaggttgt
      541 ccagcacgag cgcgacctcc gacgggctcg cgccggcgtg cgcaggcgcc ggggcgacga
      601 gctgcagcac cggttcgtcg gcctcggcgc ctgcggccct gcccatggca gcgcgcgcgg
      661 cggccacgag gtcaaccgac tgcgccggag gccgctgccc ggcgtcggcc cgggccagtg
      721 cgagcaggtc cgagagcagc gtcgagagcc gggccgcctc ctccgccacc gaacgcagcg
      781 actcctgcgc cagctccggg tcggggtgcg ccacggccac ctccgcttgc gcgcggatcg
      841 cggccaccgg cgacctgagc tcgtgcgcgg cgtcgcccgt gaaccgttcc aggcgcgcga
      901 ccgcctcgtc gcgccgtgcc agcaggaggt tgatctcctc ggccagcgcg cgcagctcgt
      961 cgcgcggtac gggcaccggc aggcgctggc cgggcggcag ggcagcggca gcggtgcgca
     1021 tccgatccac cgggcgcaac gccgccctgg tggccaccca cgctgcgagc acgacgacgg
     1081 ccccgaccac gagcgcgccg accacgaacc cgaccgcggc gtgcgcaagc agcatcgtcc
     1141 caccgacgat gtccgccgtc acgacaacga gacgggtgga gccgtccggc gtcactgtgc
     1201 tgacggccag ccatcgccgc agctccgtga gacgcccggc ggtaagtgca tcgcccgccg
     1261 cgagctcccg gacctgcttg ggccgcagcg gcaggtcggg cccgccgtcg acggggtcgc
     1321 ccgcgctgtc gacgatgcgg atctccggtt gccgcacatc ggcgggggga gttccggccg
     1381 ccagctgcgc cgctaccccc tgggcctcgc cacgcagctc ggcatcggcg gcgccgagca
     1441 tcgtgccgta gagcaggccc acacccagcc ggctcagcgc cagcagagct gcgagggcga
     1501 cgacccccac cacaagcgtg atccgcaacc gcaacgtccg cgaggcccac catcgccgca
     1561 gccgctgcgt ggggctgctc atggcggttc ggccccggcc ccgacgagca ccaagccggt
     1621 catgacgact cgacgatgta gccgtagccg cgcaccgtct tcaggacgtg cccggccccg
     1681 accgcgtcga gcttgcgccg cacgtacccg acgtacacct cgacgacgtt gcggctggcc
     1741 gcctgctcgt cgccccagac gaggcgcaac agctcgtcct tcgcgacggc cgtgtcgggc
     1801 cggcaggcga gcgcgtgcag cagcgcgaac tcacgcggcg aaagctcgac cacggcaccg
     1861 ttccagctga ccgacctgga gacggggtcg acgatcaggg accccagccg gacgcgctgc
     1921 ccggtccggc cgagtgcggc atcacggcgg cgcagcaacg ctcgcagctg cgccaccagc
     1981 acgacgaagg cgaacggctt gacgaggtag ccgtccgctc cgaggtcgag cccgtcggcc
     2041 tggtcgaact cgccgtcctt ggccgacagc agcagcaccg gcgtgtccac gccggccgcg
     2101 cgcagctgct cgagcacccg gtagcccgac aagccgggca ggataatgtc gagcacgacc
     2161 gcgtcgaagc tgccggtgag ggcggcgttg agcgcggacg gaccgtcggc gagggccaca
     2221 acctccatgc cctcggccgt gagcccccgc gcgacggccg tccggatgcc cggttcgtcg
     2281 tccaccacca gcacgcgtgc cattgccgcg gacgctaccc ggcggagggc ccgctccacc
     2341 ttggaggact ctcagcaggg atgagtcacg ctgtgcacat ggcaacgggg cagggtgacg
     2401 cgaggaacag gggcacgcgg agcaggggaa gggcaggacg cacggtcgcc gcgggcgtcg
     2461 cggtcgcggg ggcggtcggt ctcggcctgc tggcggtccc cggcggggcc ggggcggcgc
     2521 ccgagctgcc gcccgtctcg gcggccgatc tggtcggctc ggtgctggcg gcgcgccccg
     2581 gcgcgttcgg cggcacggtc gagctggaca acgcgctggg cctcccggcg ctgccgaacc
     2641 tgccgcaggc ggccaacggc acgagcaccg cgcgggtgtg gtccgacggt gagggccgcg
     2701 gccggatcca ggtccccacc aaggcgggtg agcgcacgct ggtgtccgac ggggcgacct
     2761 tctggtcctg gaactcggag gaccgcacgg tcacgcgggc accggagcac agcgcgcagc
     2821 gtccggaggg cgccgccacc gaccccacgg ccgcggccac ccaggcgctc gccgcgctcc
     2881 agcccaccag cgagatcagc gtcgatggca cggccgaggt cgcgggccgc ccggcgtacg
     2941 agctcgtgct cgccccggtt ccgtcggaac gcacactgct gcgcgaggtc cggatcgccg
     3001 tcgacgccga caagcgcgtg ccgctgcggc tgacggtgct ggccaacggc tcgtccgacc
     3061 ccgcgctgca gctcgggttc acgaatctca cgttcggtgc gcaggacccg tcgctgttca
     3121 cgttcacgcc gccgcccgga gccacggtcc aggacgagca acgtccccag ggcgggcagg
     3181 ccgggaagcc cgaggctgca gggccgacgg tcgtcggcaa gggctgggac acggtgatcg
     3241 tcacgaagaa accgccgcag gcccccgccc gcggagcgga gggtgaccgg ttcggcggcg
     3301 gcgccgacct gtccgcactg ggcacgcccg tcagcggacc gtggggcagc gggcggctga
     3361 tcaccacgac cgtggcgggc gcgatcgtca ccgacgacgg ccggatcgcg gccggcgccg
     3421 tgcccgagca ggtcctcgcc gaggccctct cccagtgacg agcagccccc gggtcgacgc
     3481 agcggctgcc ccggccggcg ccgcgccggt ccgtcccgtg gccgcgcggg ccgtcggact
     3541 ccgcaagacc ttcggccgca ccgttgcggt cgacgggatc gacctcgagg tcccggcggg
     3601 ctcggtactc ggcatgctcg gaccgaacgg ctcgggcaag accacgctca tccggatgct
     3661 cctcgggctg acgcggccca ccgcgggagc cgccgagctg ctcggccacc cgatccccga
     3721 ggagtcggcc agggccttgc cgcaggtcgg cgcgctcgtc gaggggcctg gcttccaccc
     3781 gttcctgtcc gggcgggaga acctgcgccg ggccgcggcc accgagccgc tgctcggcac
     3841 cgcggagata cgcgaggcgg tcgacgcggc gctgcaccgc gtcgggctcg gggacgcggc
     3901 ggaccgacgg ttccgcggct actcactcgg gatgaagcag cgcctcggcc tggctgcggc
     3961 gctgttgctc ccccggtcgc tcgtcgtgct cgacgagccg accaacggtc tcgaccccgc
     4021 cggcacccgc gacgtccggc agatcatcgc cgagctgcac gcagggggcg ccaccgtcat
     4081 cgtctcctcg cacctgctcg ccgaggtgga ggccacgtgc acgcacgtgg ccgtcctgca
     4141 gtcgggctcg ctcgtggcgg cgggcgagct gaacgcgctg ctggcgaccg gcaccggcgg
     4201 gctggagatc accactcccg acgtcgagct cgcgctgcga acgctgcgga gcgcgggcgc
     4261 cacgacctac cgggcggagc ggtcgcccgc cgaggagcag gccatcgtcg tgggcgatga
     4321 cggcccggcc gcgccggagg cgatcgcgct gttggtgcgg gcaggggtcg cggtgcacga
     4381 ggcgcggcgc agccggtcca acctcgatga cctgttcgcg cggctgaccg aggagtcgtc
     4441 atgacctctg cagtgctcac cggcgagccg ctctccgcgc cgcacaacgc accgctgggc
     4501 cggctgctcg ccgccgagct gcgctgggtg ctgcgccgcc cgcgcacgct ggtgatgctg
     4561 gggctcttcg cgctcgtacc cgtgctgatc tcgatcgggg tggccgtcgc gaaccgcacg
     4621 cccggacccg gggccggcct gatcggggca atagccggaa acggactggt gctgccggtc
     4681 gcggcgatga cgctggccct cgccctcctg ctgccgctcg ccgtgtcgat ggcagcagcc
     4741 gacgcgatcg cgggcgaggc agcgcacggc acactgcgcg ggctgctgat cgcgccggtg
     4801 gggcggctgc ggctcgtcgc gatgaaggcg ttcggcgtgc tcgtcgtcgc gacgctggcg
     4861 acactggccc tcgcggtgat gggcgtgctc gcggggctcg tcgtggtcgg cggggccgac
     4921 ggccagctcg tcacgctctc cggcacggcc gtcgggctcg gggacgcgct ggccagggtg
     4981 gcgctcgtcg ccgtctggac ggtgtggcag ctcgccgcgg tgggcgcggt cgcactcgcg
     5041 atctcgtcgt tcaccgagca tccgctcgtc gtgctggcct cggtgctggg cgggctgatc
     5101 gtgttcggcg tgctcagcgc gatcccggcc ctggagtggc tgcagcccta cctcctcacc
     5161 agcggctgga cggcgggcgc cgacgccctg cgcgaccccc tcccgctcga cggcatggcg
     5221 gagagctcgc tgcgcgccct ctgctacgtc gccatcggcg gcggtctgac gatccaccgc
     5281 atgctccgcc gcgacgcctg acccaccaca cctgcgcgag tcgccccaag tcaacccgcg
     5341 agtcgcccga tttcagcccg cgagtcgccc ccattccgcc cgcgagtcgc cccaaatcaa
     5401 cccgcgagtc gcccgatttc ggcccgcgag tcgcccccat tccgcccgcg agtcgccccc
     5461 aagcggcgcg agccgcgcgg gtccgcccgc gagccgcccg ggtccgcccg ccagtcgccc
     5521 ggaaccccgc gcgagtcatc cgaatgcctg cgcacgttcc ggccccgctc gcggggtgcc
     5581 gggcaggatg cacggcatga ccgaactgct ggacgacgcc gcgatcaccg ccgccctggc
     5641 cgacctgccc ggatgggagc gccagggcga cgcgatcgtg cgcaccgcga agctgccctc
     5701 gttccctgcc gccgtgtccg tggtggaccg ggtggccgag atcgcggagg ggcgcgacca
     5761 ccaccccgac atcgacatcc gctggcggac gctcacgttc cggtgctcga cgcactcggc
     5821 gggcggcatc acggagaagg acaccgcgct ggccgcatcg atctcggagc agatcgacgc
     5881 ggccggtggc tgagccccgc tagtcggcgg cggccagccc cgcgcaggcg ggcacgggca
     5941 ccttctcggc ctccgcgacg cgtctgcggc cgagctgaag cgtcagcagc agggcgatca
     6001 ccacgaggac gaggccgcca cctaccaagg gcagcaccag caggccgccc atgccgatca
     6061 cgacgccgcc gaccaccccg gcgagggcgg tgccgaggta gatcgctgag gcgttgagcg
     6121 agagcaccag gccgccggtg ggggccagct ccagcagcag cgactgcaac ggggggttga
     6181 aggcccagct gaagatgccc cacaggacga gggccatggc ggcacccacg agggtgctcg
     6241 cggtgagcga cagcgtcacg accgtgacgg cgatcccggc gagcacggcg tagagcgtgg
     6301 gcacggtgcc gaaccggtcg gccgcgcgac cgccccacgt gttgccgacc atcgccccga
     6361 cgccgtagac gaccagcagc agcgcgatgg tggcgccgtg gatgccggcc gtggcgttca
     6421 gcaggggcgt cacgtagatg aacacgacca tggcggcgag aacgccgacc acggtcatcg
     6481 cgagcaccgt cagcacgcgc cggtcagcgg cgacggcgaa gcgctcgcgc agcggcaccg
     6541 ccgggggcgc cgccattcga ggcaggcaga tcggcacggc ggccgcgacc agcgccgaga
     6601 ccactgccac cgccgcgaag acgccgccgt agccgatgct gctgccgagc aggtcgcctg
     6661 cgggaacgcc gatggcgagt gccagcgtca gaccgccgaa cacgatggcg acagcacgtg
     6721 cgcgctgagc cggcggcaga agtgcggtgg cgacgagcgt ggcggccggt gtgtaggcgg
     6781 ccgccccgat cgcggcgatg atccggccga cgatcagcag cggataggtg gtggccacgg
     6841 cggagagccc gttcccgacc gcggccgcga cgagcgcgac gagcagcacg gtgcggcgat
     6901 cccagcgccc ggccagcgtg gcgatcagcg gggcaccgac ggcgtaggcg agcgcgaaag
     6961 cggtggcgag ctggcccgcg gccgtcagcg agacgcccag ttcggagctg acggcgggca
     7021 gcacgcccga caccacgtag gcgctcgtgc cgacggcgaa ggcgccggcg gccagcaggt
     7081 aggtgcgtgc ggacatcgat ggacctctcg ggcagtgctg gacgagaact ggtacgacat
     7141 gcatcgtact acgacgttag tcgaacttcg acagatgtcg tacaatcggt ctcgtgtcca
     7201 ccgccgacca ggccatcctc cagcagccca cccgcgagga gatgcgtgtc gagcaggtac
     7261 tgcacgccct cggcgacccg gtgcgcctca tgatcgtgca ggcactcgcc gccgcacccg
     7321 agggagtggc ctgcggtgtc ataccgctac ccgtcaccaa gtccacacgc acccaccatc
     7381 tgcgcaccct gcgcgaggcg ggtgtgatct cgatgcgcac cgagggcacg cggcggatga
     7441 gcacactgcg ccgtgacgac ctcgacgcgc tgtacccggg cctgctcgac ggcgtgctgg
     7501 ccgcgccccg ctgacgcgtt cacagctggc cgcggaaccc tgcaccaggg acgatgccag
     7561 gcatgagccg ccttccccgc aagccgtacg agaaggagct gctgcgcctt cagggtgagc
     7621 tggtgcagat gcaggagtgg gtccgcacca gcggcgcgcg gttggtggtg gtgttcgagg
     7681 gccgcgacgc cgccggcaag ggcggggcga tcaagcgcgt gtcggagttc ctgaacccgc
     7741 ggatctgccg catcgtcgcc ctgcccgcac cgtcggagcg ggaacgctcg cagtggtact
     7801 tccagcgcta caccgagcac ctccccgcgg caggggagat cgtgctgctg gaccggagct
     7861 ggtacaaccg cgccggcgtc gagcgggtga tggggttctg cactcccgac gagtacgccc
     7921 gcttcctgcg ccagtgcccc gtgttcgagg agctgctcgt cgaggacggg atcctgctgc
     7981 gcaagtactg gttctcggtc agcgacgccg agcaggagcg ccgcttccgc ggccggatga
     8041 gcgatccgat gcgtcagtgg aagctctccc cgatggacct cgagtcgatc acgcggtggg
     8101 aggactactc ccgggcgaag gacgagatgt tcgtccacac cgacaccgcc gccaccccgt
     8161 ggttcgtggt ggaggccgag gacaagcggc gcgcccggat caacatgatc gcgcacctgc
     8221 tcgcgaccgt cccgtggcag ccggtggcat cgcgcgacct ggagctcccg caacggccac
     8281 cgcggcgcgg ctaccagcgc ccgccacgcg atcgcttcaa cgaggtgccc gaccacgcgg
     8341 ccatgctcgt gggctgacgg ccaccgtgcc gtgtcggcga cccgttcgag aggcgctcag
     8401 aaccggcggt tggccagcac cggtaccgcg cggcgcgcct gctccacctc gtccatggcc
     8461 aggtcgacga gcagcaggcc gggctcggcg ccgagctggt cgagcacgac gcccagcggc
     8521 ccgacggcga ggctcgcccc gatccccgtc ggcgcgctgc ccgcatcccg gccgacggtc
     8581 gtcgggtcgg cctggtcgca cgccaccacg aacgcggtgc tgtcgagggc cctggcccgg
     8641 accagcaggt cccactgctc gcgcttgccc gggccggcgc cccacgacgc cggcaccagc
     8701 acgacggtgg caccgttgcc cgcaagctcc tggaagagcc ccgggaagcg caggtcgtag
     8761 caggtggcga gaccgaccgt gacctcgtcg acgacgatgc tcaccggctt cgaccccggg
     8821 gcgacagtgc gcgactcggc gaatccgaac gcgtcgaaca ggtggatctt gtcgtaggcc
     8881 gcctccacac caccgccggt cgccagcaac gtgttgtgca cacggccgtc gtcggccggg
     8941 gtgaacatgc cggcgacgac ggtgacccct gcggcgtcgg cgatcgcccg gacccgccct
     9001 gcccacggcc cgtccagcgg ctcggccacc ggaccgagcg gtaccccgaa gcagcacatg
     9061 gccgcctccg ggaacaccac gacccgtgcc cccgccgccg cggcccggcg tgcgccgtcc
     9121 tcgatcaggc cgaggttgtg g
//
LOCUS       DASRSC010000211        11426 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5807475,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000211 DASRSC010000000
VERSION     DASRSC010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11426
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5807475"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1029)
                     /locus_tag="VFE39_10385"
     CDS             complement(43..1029)
                     /locus_tag="VFE39_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HET6259046.1"
                     /translation="MARPALLHRVPATPPVLMRRLAIAAVVAQAGIAVTGSVVRVTGS
                     GLGCPTWPECFPGSLVPAPHPEVAALTQWIEFSNRLLTFAVVLIAGTCLIAALATRPR
                     RARLTRLALVQPLGVVAQAVIGGITVLTGLAWWTVSVHFLVSMVLVWLGMQLVDTATD
                     GDGPPRRTVRGAVRGLVATSTAVLAALLAAGTLVTAAGPHAGDAATPRLGIGVPAAAQ
                     LHADLLFAYLGMLVGLGFALAAVDASQRLRRRYVWLIVAVVAQGALGGIQYALGVPEA
                     LVSLHVLGAALVTVGAAALWSGTTERPNVVSADVPADAPGATQPEPALADRG"
     gene            1400..2281
                     /locus_tag="VFE39_10390"
     CDS             1400..2281
                     /locus_tag="VFE39_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6259047.1"
                     /translation="MWQVTVLAGGLALLAAALFAVSTVAQQRSASTVPDDTAHGLGLI
                     RVLVRRPQWWAGVVGDGGGYLVQAAALGFGSLLLVQPLLVTTLLFALPLGAAWAGRAL
                     AREDWLWSVLLAVSLAVFVVTGNPTDGVDRAPVGAWLPTGAILAGMFVVCVVAAARSR
                     GTARAVLLAAATAVSYGVAAALTKGVVGQLDEGLVALLTSWETYALGIVSCAGTLLQQ
                     SAFQAGDLGASLPTMIVGEPVVAVVIGVTVLQEKLRANGAEWVLIALLVVVMVAATTA
                     LARSSARTSAVPRPAPV"
     gene            complement(2337..5783)
                     /gene="lysS"
                     /locus_tag="VFE39_10395"
     CDS             complement(2337..5783)
                     /gene="lysS"
                     /locus_tag="VFE39_10395"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET6259048.1"
                     /translation="MSSGTEADRHPADQREHRLHKLAAARAGHGLPYRYPTDHTAADI
                     RTTHVGLGPDAFTTDRVRVAGRVDLIRRHGGLSFAVLRDRTGTIQLFVDTAVLGPDGH
                     RAFDDLDRGDWVGAEGTVMTTRRGELSVCVESFALLGKSLLEASDKHRGLSDVETRYR
                     QRYVDLAANERTREIFRIRHTAVRAIRRHLEDRGFAEVEGPVLQTIQGGASARPFVTH
                     HNALDLDMYLRIALELHLKRLIVGGLERVFEIGRVFRNEGIDTRHNPEFTMLEAYQAF
                     ADYHDMMDLVEGMIIAAARAALGPDDELVVHYGGCSMDLGAAPWPRVRFADMIAEKTG
                     ATMHPGMPVEDARAVLDRLRIPYERGWGAGRLMKEVYDEKVQHDVVGPVFCIDYPREV
                     SPLARTHRDDPDYVERFELIVAGFELCNAYSEQNDPVEQLAAFEAEARAKAGGDPEAG
                     DIDLDYIRALEHGMPCTGGLGVGIDRLVMLLAGVDSIREVILFPTLRPDFAVPPGSGP
                     HGAPRPVLPPTPVAPGTAAGQRTGAPSGNGLPASAAGPLMALPDLAPAAHPIPPPERP
                     PERSRLAVRVIAVLTALGGVLQLLAQLPLVHARMNVRDELGQLWLPVAGHVVSVIVGL
                     LLILLADQLAKRKRLAWQLAVVAFSVGGFAHMAKGPHPVAITICVAMLVALVWHRELF
                     RAPADPPSLLRLLRFVPLYLAGVLAFGFVALGVERDRIDPGLTLGGGLVTIGGGLIGI
                     DGPYTYRSPFFAAFFPVALVALGVAGLLVFAILVFRPLVARHPHTESDWEHANRLVHT
                     YGWDTLAYFSLRDDKSFFFSSDGEAVLAYTYLGGYALVSGDPIGERTSVVQVLDEFLA
                     MCDERAWNPAFLAVREASMPLYASRGFTSFYLGDEAILDCARFSLEGPGRKSLRAAVR
                     RVGRSYRFQLVTESNASPRLVEQLNAISAKWRGKAPERGFTMSLSQDVTGAEANREFL
                     LCVALDEDGAPGGFLRLVPAFGPSFGYTLDLMRHDPAAPNGMTEFLIAGAAAALGQRG
                     ICRLSMNFAMWGRLFADDITFSPAQRAARWAVGVLNPFFQIRSLHDFNAKFDPEWMPR
                     VLAYRHRIDLPRVGLLYAGAEGFLALPVIGELLVPKAVGGVAAPSAPGPAPERPTGTR
                     AA"
     gene            complement(5953..8127)
                     /locus_tag="VFE39_10400"
     CDS             complement(5953..8127)
                     /locus_tag="VFE39_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HET6259049.1"
                     /translation="MVHRALRVTAAACVGFYLCRYVLGDAETAVYALFGAIALGALSE
                     VTGASGQRTRTYLAALLVGLVLVSLGTMLAASTWAAAAGMLVVGFAVTYAGVGGPRVA
                     GVANGLQLFYVLPCFPPFAPGSLDDRLIGLTVGVLLLAVADRVLWPASAPPDVALRVA
                     DAAGAAAAYARALQAATVAGDPAGPAFRCTDLRAEAGHAADALRLSNLPMTGRPTGPS
                     VHDRSLTRAGVAVRMAIARLGTSADRLGTRDAVEPEAATLEMLPETAQLLGADADALE
                     AVRDALRGTGPPPAPDVVERARRGYVEHGMRRLAAGVDPEPWLRAALAVVAVTEALRT
                     LVLAVRGTVGASVPPPADTPDDFWFLHASPGKLWWERLRAHLTPRSVYLQNAVRLALG
                     LAAARLVAGAFDLSHGFWVLLATLSLMRTSAVAGRAVLVRAFSGTVAGAVAAGLVLTF
                     VGSDTTVYAWVTPLLMIVAFAAGPLLGLAAAQAGFTLVVAVVFAQVAPATWQLAADRL
                     LDVVVGGLIGAVIGAAVWPRGGSGEVRRVAAASLDAGADEIVATAFFLTSAGGAPPST
                     ALTRLSVMFDHTYSQYRTEPSGLGPEPDWLVVLAVVHRIGDRAQTMRVRHPEADQLPW
                     PDFASQLRADAADVAAAYRGAAAEIAASRPPPAGVAEQLRERIAAQHLTVTFTDSPAE
                     ALRAFDGWGWIHALVDELERIEHALSPMARSNARPQHTTTSR"
     gene            complement(8326..9678)
                     /gene="zwf"
                     /locus_tag="VFE39_10405"
     CDS             complement(8326..9678)
                     /gene="zwf"
                     /locus_tag="VFE39_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET6259050.1"
                     /translation="MTAPVPAAGALVLFGVTGDLARKMILPALYRLTERGELTVPVIG
                     IARTDWDTDALRKHVGESVRAVLPDVDDAVLERLQRAVHLVAGDYGDPTTFDRLATTL
                     REQAGADAFAVHYLAVPPGLFGTVADGLAAVGLNEQARLVVEKPFGHDLASARALNAQ
                     LRRNFDDDRLFRVDHYLGKEPVEDLLVLRFANTVIEPLWNRTWIDHIDITMAEDFDVA
                     DRGSFYDAVGTVRDVVQNHLLQVLAYLTMEAPQSDSAEHQRDAKHRLLQAVRAVDPAE
                     VVRGQYTGYRDVPGVAPDSTTETYVALTLHIDDDRFTGVPVHIRAGKALPETVLDLVV
                     VLRSPPRPLFHGAAQDPPPNRIRLRLQPDAGVTFYLLAKQPGGGDIATELPVSVDFRQ
                     VLGPVHAAYERIFADALSGNPAHFARMDTLEEAWRVVEPILDPGTAPLPYAPGTWGPT
                     "
     gene            complement(9675..10697)
                     /gene="gnd"
                     /locus_tag="VFE39_10410"
     CDS             complement(9675..10697)
                     /gene="gnd"
                     /locus_tag="VFE39_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010314636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HET6259051.1"
                     /translation="MRIAMVGLGRMGANMVRRLLRDGHECVVHDISEKAVQQLVDEGA
                     QGAGSLKELVGALAPPRTVWLMLPAAITGSTVIDLLDLLDPGDAVVDGGNSSYRDDID
                     RAEVAAVHDIDYVDCGTSGGVWGLDRGYCLMIGGPDRAVDRLDPIFASLAPGVGTAGR
                     TPGRTGEVSRAEHGYLHCGPSGAGHFVKMVHNGIEYALMAAYAEGLNVIRGAGAGKHQ
                     AEADAETAPLREPRYYPYDGIDLAEVTEVWRRGSVIGSWLLDLTAEALAADPALEQFT
                     GRVSDSGEGRWTSIAAIEEGVSAPLLTAALYSRFASRGSELFADKVESAQRKEFGGHA
                     EKPAAS"
     gene            11042..>11426
                     /locus_tag="VFE39_10415"
     CDS             11042..>11426
                     /locus_tag="VFE39_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HET6259052.1"
                     /translation="MTAVSPKPVTPRPYPARRPVKGSTLVKMLHTTDPKDIAILYLVT
                     SFGFFLVGGLMALLMRAELAVPGQQFLSNEQYNQLFTQHGTIMLLLYATPILFGFANY
                     IVPLQIGAPDVAFPRLNAFSYWLFLF"
BASE COUNT         1638 a         4018 c         4244 g         1526 t
ORIGIN      
        1 cgatccgcac gtcatgcgcc tgcggggggc gatggtgcgg agtcagcccc ggtcggcgag
       61 ggccggctcc ggctgggtgg caccgggggc gtcggcgggg acgtcggcgg atacgacgtt
      121 cgggcgctcg gtggtgccgg accagagcgc cgcggccccg accgtgacga gcgcggcccc
      181 gaggacgtgc agcgacacca gcgcctcggg aacgccgagt gcgtactgga tgccgccgag
      241 agcgccctga gccacgacgg cgacgatgag ccacacgtag cgccgccgca gtcgctgcga
      301 tgcgtccacg gccgccagcg cgaacccgag tccgacgagc atcccgagat aggcgaagag
      361 caggtcggcg tgcagttgcg ccgccgcggg cacgccgatg ccgaggcggg gcgtcgccgc
      421 gtcgcccgca tgcggtccgg cggcggtcac gagggtgccg gcggcgagca gtgcggccag
      481 cacggccgtg ctcgtggcga ccagcccgcg gacggccccc cggacggtcc gccgcggcgg
      541 accgtcgccg tccgtcgcgg tgtcgacgag ctgcatgccc agccacacca gcaccatcga
      601 gacgaggaag tgcaccgaca cggtccacca ggccaggccg gtgagcaccg tgatcccgcc
      661 gatcaccgcc tgcgcgacca cgccgagagg ctgaaccagc gcgagccggg tgagccgcgc
      721 acggcgcggg cgggtggcca gcgccgcgat gaggcaggtg ccggcgatga gcaccactgc
      781 gaacgtgagc agccggttgc tgaactcgat ccattgcgtc agcgccgcca cctccgggtg
      841 cggcgcgggc accaggcttc ccggaaagca ctccggccac gtcggacagc cgagcccgga
      901 gcccgtcacc cgcaccacgg acccggtgac cgcgataccg gcctgggcga cgacggcggc
      961 gatggccagc cggcgcatca gcacgggcgg ggtcgcggga acacggtgga ggagtgcggg
     1021 tcgggccacc ccctgatggt aggtgggcgt caccaggccg cgacagcctg ctcgaaggct
     1081 cctgcaggct gcaccagtat ctgacggacg ccgagcctgc cgtcgacgcc gaccccggag
     1141 aagtcccgcg cagcgctcac ggcggagcgt agatcgtcgg gcggccggcg cactcggctc
     1201 gttcagcagg cggcgaccga cgtcgacgcg gtcgtcgagc atgcgggtcc gtcgacgcgg
     1261 tcgtcgagca tgcgggtcga cgccaccgca cacagtgcgg cgaccgcgca cgcgacaggc
     1321 ggtgtgcacg ctgacccacg ggtgcgcgct gcgcaacgcg gccgtccgac cagcgtcgat
     1381 gtggccggca gcaggcatga tgtggcaggt gacagttctc gcgggagggt tggcgttgct
     1441 cgcggccgcc ctcttcgccg tctcgacggt cgcgcagcag cggtccgcgt ccaccgtgcc
     1501 ggacgacacc gcccatggcc tcgggctgat ccgcgtgctg gtccggcgcc cgcagtggtg
     1561 ggcgggggtg gtcggcgacg gcggcggcta cctggtgcag gcggccgcgc tggggttcgg
     1621 ttcgttgctg ctggtacagc cgctgctggt cacgaccctg ctgttcgcgc tgccgctggg
     1681 ggcggcgtgg gcaggccggg cgctcgcgcg ggaggactgg ctctggtccg tgctcctcgc
     1741 ggtgtcactg gcggtgttcg tggtcacggg gaatccgacc gacggggtcg accgcgcccc
     1801 cgtcggtgcg tggctgccga cgggcgccat tctcgccggg atgttcgtgg tgtgcgtcgt
     1861 ggccgcggcc cggagccgag gcacggcgcg cgccgtgctc ctcgccgccg ccaccgccgt
     1921 gtcgtacggg gtggcggccg cactgaccaa gggggtcgtc gggcagctcg acgaggggct
     1981 cgtcgccctg ctgaccagct gggagaccta cgcgctcggc atcgtctcct gcgcgggcac
     2041 gctgctgcag cagtccgcgt tccaggcggg cgacctcggg gcatcgctgc ccaccatgat
     2101 cgtgggggag ccggtcgtgg cggtcgtgat cggggtgacc gtgctgcagg agaaactgcg
     2161 tgccaacggc gccgagtggg tgctgatcgc cctgctcgtc gtcgtgatgg tggcggcgac
     2221 cacggcgctg gcccgctcgt cggcgcggac gagcgcagtg ccgcgacccg cgccggtctg
     2281 aacccggcga gctacgccgg ctccagaccg tccggctcca cgaggggcga gcagggtcac
     2341 gctgccctcg tcccggtcgg cctctccggt gcggggcccg gtgcggacgg cgcggccacg
     2401 ccccccacgg ccttcggcac cagcagctcg ccgatcacgg gcagggcgag gaaaccctcg
     2461 gcgccggcgt agaggaggcc gacgcgcggt aggtcgatgc ggtggcggta ggccagtacc
     2521 cggggcatcc actccgggtc gaacttggcg ttgaaatcgt gcagcgacct gatttggaag
     2581 aacgggttga gcaccccgac ggcccagcgg gccgcgcgct gggcggggct gaacgtgatg
     2641 tcgtcggcga aaagccgtcc ccacatcgcg aagttcatcg acagccggca gatgccacgc
     2701 tgccccagcg ccgccgccgc cccggcgatc aggaactcgg tcatgccgtt gggggcggcg
     2761 gggtcgtggc gcatcaggtc gagcgtgtag ccgaaggacg gcccgaaggc gggcacgagg
     2821 cgcaggaagc cacccggcgc accgtcctcg tcgagcgcca cgcacagcag gaactcacgg
     2881 ttggcctcgg cgccggtgac gtcctgggac aacgacatgg tgaacccgcg ctcgggggcc
     2941 ttgccccgcc acttcgcgct gatcgcgttg agctgctcca ccagccgggg cgaggcgttc
     3001 gactcggtga ccagctggaa gcggtagctg cgtccgacgc gacgcaccgc cgcgcgcagg
     3061 ctcttgcgcc ccggcccctc cagtgagaac cgcgcacagt cgaggatcgc ctcgtcgccg
     3121 aggtagaagc tggtgaaccc acgggaggcg tagagcggca tgctggcctc gcgaacggcg
     3181 aggaaggcgg ggttccaggc ccgctcgtcg cacatggcga ggaactcgtc cagcacctgg
     3241 acgaccgacg tccgctcccc gatgggatca ccggagacca gcgcgtagcc gccgagatag
     3301 gtgtaggcga gcacggcctc gccgtcggag gagaagaaga agctcttgtc gtcgcgcagc
     3361 gagaagtagg cgagggtgtc ccagccgtag gtatggacga gccggttggc gtgctcccag
     3421 tcactctcgg tgtgcgggtg gcgtgccacc agcggccgga acaccaggat cgcgaacacc
     3481 agcagcccgg cgacaccgag cgccaccagc gccacgggga agaacgcggc gaagaacggt
     3541 gaccggtagg tgtaggggcc gtcgatcccg atgagcccgc cgccgatggt gacgagcccg
     3601 ccgccgaggg tgagcccggg gtcgatgcgg tcacgctcga cgccgagagc gacgaacccg
     3661 aaggcgagca cgcccgccag gtagagcggc acgaaccgga gcaggcgcag cagcgatggg
     3721 gggtcggccg gggctcggaa gagctcgcgg tgccacacca acgccaccag cattgcgacg
     3781 cagatcgtga tcgcgacggg gtgcggtccc ttcgccatgt gcgcgaaccc gcccaccgag
     3841 aaggcgacga cggccagctg ccacgccagg cgtttgcgtt tggccagctg gtcggccagc
     3901 aggatcagga gcagcccgac gatcacggag acgacgtgcc ccgccaccgg gagccacagc
     3961 tgcccgagct cgtcgcgtac gttcatccgg gcgtgcacga gcgggagctg ggcgagcagc
     4021 tgcaacaccc ctccgagcgc ggtgagcacg gcgatgaccc gcacggcgag ccggctgcgc
     4081 tcgggaggtc gctccggcgg cgggatcggg tgcgccgccg gggcgaggtc gggcagcgcc
     4141 atgagcggcc cggccgccga cgcgggtagc ccgttcccgg acggggcgcc cgtccgctgg
     4201 cctgccgcgg tcccaggcgc caccggggtg gggggcagca cggggcgggg cgcgccgtgt
     4261 ggtccgctcc ccggaggtac ggcgaagtcc ggccggagcg tcgggaacag gatcacctcg
     4321 cggatcgagt cgacgccggc cagcagcatg acgagacggt cgatcccgac tccgaggccg
     4381 cctgtgcacg gcatgccgtg ctccagtgcg cggatgtagt cgaggtcgat gtcgcccgcc
     4441 tccgggtccc cgcctgcctt cgctcgggcc tccgcctcga aggcggcgag ctgctcgacc
     4501 gggtcgttct gctcggagta ggcgttgcac agctcgaacc cggcgacgat cagctcgaaa
     4561 cgctccacgt agtcggggtc gtcgcggtgc gtcctggcga gcggggagac ctcgcgcggg
     4621 tagtcgatgc agaagaccgg cccgacgacg tcgtgctgca ccttctcgtc gtagacctcc
     4681 ttcatgagcc ggcccgcgcc ccagccgcgc tcgtacggga tgcgcagccg gtcgaggacg
     4741 gccctggcgt cctcgaccgg cataccggga tgcatcgtgg ccccggtctt ctcggcgatc
     4801 atgtcggcga accggacccg cggccacggt gcggctccca ggtccatgga gcacccgccg
     4861 tagtgcacga ccagctcgtc gtcggggccg agggccgccc gcgccgcggc gatgatcatg
     4921 ccctcgacga ggtccatcat gtcgtggtaa tcggcgaacg cctggtaggc ctcgagcatc
     4981 gtgaactcgg ggttgtgccg ggtgtcgatg ccctcgttgc ggaacaccct gccgatctcg
     5041 aagacccgct cgagaccgcc cacgatcagc cgcttgaggt gcagctccag cgcgatgcgc
     5101 aggtacatgt cgaggtcgag cgcgttgtgg tgggtgacga acggccgcgc cgacgcgcca
     5161 ccctggatcg tctgcagtac cgggccctcg acctcggcga agccccggtc ctccaggtgc
     5221 cggcggatcg cgcggacggc ggtgtgccgg atgcggaaga tctcgcgcgt ccgctcgttg
     5281 gcggcgaggt ccacgtagcg ctgccggtag cgggtctcga cgtcgctgag tccgcgatgc
     5341 ttgtcggagg cctcgagcag tgactttccc agcagggcga agctctcgac gcagacagag
     5401 agctcgcccc gccgggtggt catcacggtg ccctcggcgc cgacccagtc gccccggtcg
     5461 aggtcgtcga acgcccggtg gccatcgggc ccgaggacgg ccgtatcgac gaacagctgg
     5521 atcgtgccgg tccggtcgcg cagcacagcg aagctcaggc ccccgtgccg ccggatgagg
     5581 tcgaccctgc ccgcgacgcg cacacggtcc gtcgtgaagg catcgggacc cagacccacg
     5641 tgtgtcgtcc ggatgtcggc ggccgtgtgg tcggtggggt agcggtaggg gagcccatgc
     5701 ccggcgcgcg ccgccgcgag cttgtgcagc cggtgttcgc gctggtccgc gggatgccgg
     5761 tcggcttcag taccggagct caccgtcgcc acgataaggt cgtcggtcgt tcgcgcctag
     5821 ggttcccgat gtccccgatc ggagtgcgct ccaggcggac cggctccctc acgcgaagac
     5881 gagcgcgagg tgggccgcgg cgcggaactc tccgccggcg tcccgcacca tcggaggccg
     5941 ttcctcccgt gctcagcggg acgtcgtcgt gtgctgcgga cgcgcattcg accgcgccat
     6001 cgggctcagg gcgtgctcga tccgctccag ctcgtccacc agcgcgtgga tccaccccca
     6061 gccgtcgaac gcccgcagcg cctccgcagg cgaatcggtg aacgtgacgg tcaggtgctg
     6121 cgccgcgatc cgctcgcgga gctgttctgc tacccctgcc gggggcggcc tgctggccgc
     6181 gatctccgcg gctgccccgc ggtaggccgc agcgacatcc gccgcgtcgg cccgcagctg
     6241 gctcgcgaag tcgggccacg gcagctggtc ggcctccggg tgccgcaccc gcatggtctg
     6301 cgcgcggtcg ccgatgcggt gcaccacggc gagtacgacg agccagtccg gctcgggacc
     6361 cagcccgctg ggctcggtgc ggtactgaga gtaggtgtgg tcgaacatca ccgacagcct
     6421 ggtgagtgcc gtcgacggcg gcgccccgcc cgcgctggtc aggaagaagg cggtggcgac
     6481 gatctcgtcg gcgccggcgt ccagggacgc cgcggccacc cggcggacct ccccgctgcc
     6541 accgcgcggc cacacggccg ccccgatcac cgccccgatc aggccgccga cgacgacgtc
     6601 aagcagccgg tccgcggcga gctgccacgt agcgggtgcg acctgggcga agacgacggc
     6661 gacgacgagg gtgaaacctg cctgcgcggc tgccagcccg agcagtggac ccgccgcgaa
     6721 cgccacgatc atcagcagcg gggtgaccca tgcgtacacc gtggtgtcgg accccacgaa
     6781 ggtgagcacg agcccggcgg cgaccgcccc cgcaaccgtg ccggagaacg cccgcaccag
     6841 cactgctcgg ccggcgacgg ccgaggttcg catcaggctc agcgtggcga gcagcaccca
     6901 gaaaccgtgc gacaggtcga acgcgcccgc cacgaggcgg gcagcggcca ggccgagcgc
     6961 gagccggacc gcgttctgca ggtagaccga tcgcggggtg aggtgcgcgc gcaggcgttc
     7021 ccaccagagc ttgccggggg aggcgtgcag gaaccagaag tcgtcgggag tgtccgccgg
     7081 cggcggcacg gaagccccga cggtgccgcg gacggccagc acgagtgtgc ggagtgcctc
     7141 ggtgaccgcg accaccgcga gcgcggcgcg cagccacggt tcggggtcga ccccggcagc
     7201 cagccggcgc atcccgtgct cgacgtagcc cctgcgggcg cgctccacga cgtccggggc
     7261 gggcggcggg ccggtaccgc gcagcgcgtc gcgcaccgcc tccagcgcat cggcgtctgc
     7321 gccgaggagc tgtgccgtct cggggagcat ctcgagggtc gccgcctcgg gctccacggc
     7381 gtcccgcgtg cccagccggt ctgctgatgt tccgagccgg gcgatggcca tgcgtacggc
     7441 gacgcccgca cgggtgaggc tgcggtcgtg cacgctgggc ccggtcggtc gccctgtcat
     7501 cgggaggttg ctcagccgga gggcgtcggc ggcgtggccc gcctccgcgc gaaggtcggt
     7561 gcagcggaaa gccgggccgg ccggatcgcc ggccacagtt gccgcctgca gcgcacgcgc
     7621 gtacgcggcc gcggcacctg cggcgtcggc gacacgcagg gccacgtccg ggggcgccga
     7681 agcgggccag agcacccggt cggcgaccgc gagcagcagc accccgaccg tgaggccgat
     7741 gaggcgatcg tcgagcgagc cgggggcgaa cggcgggaag cacggcagca cgtagaacag
     7801 ctggagcccg ttggcgacgc ctgccacccg cggaccgccg acgcccgcgt aggtcaccgc
     7861 gaacccgact accagcatcc ccgcggcggc cgcccaggtg ctcgcggcga gcatggtgcc
     7921 gagcgagacg aggacgaggc cgacgagcag cgccgcgagg taggtgcggg tgcgctggcc
     7981 tgatgccccg gtgacctcgg acagtgcgcc cagcgcgatc gccccgaaca gggcgtacac
     8041 agccgtctcg gcatccccga ggacgtaacg gcagaggtag aagcccacgc aggcggccgc
     8101 ggtgacgcgt agggcccggt ggaccacgac gagaccaggg tcgtggcggt tcagccacga
     8161 ccgaagcccg ccgaggtgcc cctcggtccc ccggccctcc ccggccggcc gcactacagc
     8221 agtgggggtg gcgccggacc ggcgtcgcgg ctcatcgcac tccctcgctg cgctcggtcg
     8281 gcgctgcgcg aaggcgctgt gacactgatt cgcgcgcaag ctcgctcatg tcgggcccca
     8341 cgtgccgggc gcgtacggga gcggcgcggt gcccgggtcg aggatgggct cgacgacccg
     8401 ccacgcctcc tcgagcgtgt ccatccgcgc gaaatgtgcc gggttgccgc tcagcgcgtc
     8461 ggcgaagatc cgctcgtacg ccgcatgcac cggtccgagc acctggcgga agtcgaccga
     8521 caccggcagc tcagtggcga tgtccccacc gccgggctgc ttggcgagca ggtagaaggt
     8581 caccccggcg tcgggttgca ggcgcagccg gatcctgttg ggtggcgggt cctgcgctgc
     8641 gccgtggaac agcgggcggg gcggtgaccg gagtacgacg accagatcca gcaccgtctc
     8701 cggcaaggcc ttgccggccc ggatgtggac cggcacaccc gtgaagcggt cgtcgtcgat
     8761 gtgcagggtg agggccacgt acgtctcggt cgtcgagtcg ggggcgacgc cggggacgtc
     8821 gcggtagccc gtgtactgcc cgcgcaccac ctcggccggg tcgaccgcgc gaacggcctg
     8881 caggagccgg tgcttcgcat cgcgctggtg ctcggcggag tcgctctgcg gggcctccat
     8941 cgtcaggtag gcgagcacct gcagcaggtg gttctgcacg acgtcgcgga ccgtgccgac
     9001 ggcgtcgtag aaggagccgc ggtccgcgac gtcgaagtcc tcggccatcg tgatgtcgat
     9061 gtggtcgatc caggtgcggt tccacagtgg ttcgatcacc gtgttggcga agcggagtac
     9121 cagcaggtcc tccaccggct ccttgccgag gtagtgatcg acgcggaaca gacggtcgtc
     9181 gtcgaagttc cggcgcagct gggcgttcag cgcgcgggcg gaggcgaggt cgtgaccgaa
     9241 gggtttctcc acgacgagcc gggcctgctc gttgagcccg accgcggcga gcccgtcggc
     9301 caccgtgccg aacagcccgg gcggcacggc caggtaatgc acggcgaatg catcggcgcc
     9361 cgcctgttcg cgcagggttg tcgcaagccg gtcgaacgtc gtggggtcgc cgtagtcgcc
     9421 cgccaccagg tgcacggccc gctgcagccg ttcgagcaca gcgtcgtcga cgtcgggcag
     9481 cacggcgcgc acgctctccc cgacgtgttt gcgcagcgcg tcggtgtccc agtcggtgcg
     9541 ggcgataccg atgaccggca cggtgagctc accccgctcg gtgagccggt acagggcggg
     9601 caggatcatc ttccgcgcaa ggtcaccggt gacgccgaag agcaccagtg cgcccgctgc
     9661 gggcactggc gcggtcaaga cgcggccggc ttctcggcgt ggccgccgaa ctccttgcgt
     9721 tgcgccgact ccaccttgtc ggcgaacagt tcactgccgc gcgaggcgaa ccggctgtac
     9781 agcgcggcgg tgagcagcgg cgcggagacg ccctcctcga tggccgcgat ggacgtccag
     9841 cggccctcgc cggagtcgga gacgcggccg gtgaactgct cgagcgccgg atcggcggcc
     9901 agtgcctcgg ccgtgaggtc cagcagccac gagccgatga cgctgccgcg gcgccagacc
     9961 tcggtgacct ctgcgaggtc gatgccgtcg taggggtagt agcgcggctc gcgcagcggc
    10021 gcggtctcgg cgtcggcctc cgcctggtgc ttgccggccc cggcgccgcg gatcacgttc
    10081 aagccctcgg cgtaggccgc catcagggcg tactcgatgc cgttgtgcac catcttgacg
    10141 aagtgcccgg cgccggacgg accgcagtgc aggtagccgt gctcggcgcg ggacacctcc
    10201 ccggtgcggc caggggtgcg tccggcggtg ccgacgccgg gggcgagcga cgcgaagatg
    10261 gggtcgagcc ggtcgactgc acggtccggg cccccgatca tcaggcagta cccacggtcg
    10321 agcccccaga caccgccgct ggtgccacag tccacgtagt cgatgtcgtg gacggcggcg
    10381 acctcggcgc ggtcgatgtc gtcgcggtag gaggagttgc cgccgtcgac gactgcgtca
    10441 ccggggtcga gcaggtcgag caggtcgatc accgtggatc cggtgatggc cgccgggagc
    10501 atgagccaca ccgtgcgcgg tggcgcgagc gcgccgacga gctccttgag cgatcccgct
    10561 ccctgggcgc cttcgtcgac cagctgctgc accgccttct ccgagatgtc gtggacgacg
    10621 cactcgtggc cgtcacggag caggcggcgc accatgttcg cgcccatccg gcccaggccc
    10681 accatcgcga tcctcatgct cggctcctct gcctcggact gcatctgcct cgactgcatc
    10741 tgcctcgggc gctatcggcc tcgggtactg caccgtcccg ccgttcaggg gtcgtcgacc
    10801 gcctcgtaca ggtggcatcg ccagtgccac tcgtcgacgc cggccccggt cgcgatacat
    10861 cggccaacgg aacggggctc cgtgcgcgaa agtaccgcgc tctgctcctg acgcacggcg
    10921 agtgggcgca acgacggacg acagtgccaa cgaacgggtg tcgacagcgc gcctcacagc
    10981 tcctaccgtc ctcacacgcg gacttcggcg tcgagcgcgg gctcacgcac caggaggcac
    11041 catgaccgct gtctctccga agccggtcac cccgcgcccc tatccggcgc gccggccggt
    11101 caagggatcc acgctggtga agatgctgca caccacggat cccaaggaca tcgccatcct
    11161 gtatctggtg acgtcgttcg ggttcttcct ggtcggcggg ctgatggcgc tgctgatgcg
    11221 cgccgagctg gcggtgccgg ggcagcagtt cctgtccaac gagcagtaca accagctgtt
    11281 cacgcagcac ggcacgatca tgttgctgtt gtacgcgacg ccgatcctgt tcggcttcgc
    11341 gaactacatc gtgccgctgc agatcggtgc acccgacgtc gcgttcccgc ggctcaacgc
    11401 cttctcgtac tggttgttcc tgttcg
//
LOCUS       DASRSC010000212         4701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3843992,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000212 DASRSC010000000
VERSION     DASRSC010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4701
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3843992"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VFE39_10420"
     CDS             complement(<1..894)
                     /locus_tag="VFE39_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HET6259053.1"
                     /translation="MAEVLVLVDHLEGEIKKSTFELLTAARTLGEPSAVVVGPAGTAA
                     KLGGALKEHGAQKIYVAETDSTEFLTPEVGVLASLIESKDPAAVLIAVSADGKEIAGR
                     VAVRTNSGLLGDVVAVSPEGVTHSVFGGAYTAEAKATTAHPVITLRPGSVEIEPSAGA
                     GAEETVPVPAAHGRVATVTSREPIVGGDRPELTEASVVVSGGRGVGSADDFQVVEALA
                     DSLGAAVGASRAAVDSGYYPHQFQVGQTGKTVSPQLYIALGISGAIQHRAGMQTSKTI
                     IAVNKDAEAPIFEVADFGVVGD"
     gene            complement(924..1709)
                     /locus_tag="VFE39_10425"
     CDS             complement(924..1709)
                     /locus_tag="VFE39_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HET6259054.1"
                     /translation="MNIVVLVKQVPDTYSERKLNSSDGTLDRDATDAVLDEINERAVE
                     AALQLKEAHDGTVTVLTMGPDRATDAIRKALSMGADKAVHLSDEALHGSCAVQTARAL
                     AKALGTVEGGFDLVVAGNEASDGRSAAVPAMVADVLGLPSLTHAREVTVDGSTVTVRR
                     ETDDGVTHLTAELPAVVSVGEKINEPRYPSFKGIMAAKKKPVSTISLADAGIDPSEVG
                     LANALTSVTSSQPKPPKSAGEKIEDEGDGGAKVAAFLVSQKLI"
     gene            1971..2102
                     /locus_tag="VFE39_10430"
     CDS             1971..2102
                     /locus_tag="VFE39_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259055.1"
                     /translation="MDRIRSFLAGPQGRRLTEQGRRMAADPRNQERARGLLARFRRR"
     gene            2185..3159
                     /locus_tag="VFE39_10435"
     CDS             2185..3159
                     /locus_tag="VFE39_10435"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HET6259056.1"
                     /translation="MSVLVRAHPSRRSGTIRTWGAAMTLAVVASACGNPTGGEVSLEP
                     HTAPGQPAGTMLLATGTDFAAIVQRVQEAISETGGSVTTVVDHAGDARAAGVAIPPST
                     LVIGGSPAAQLPLLRIDQRAGADLPQRYLVRQGSDGSVSLTVDSADYVAAVAGVSDPG
                     ARNALRDSTAAVLGEAVPGTPVPVPSPLVGVTPSNYLLTVVGSASVPTTVDRLRRGAD
                     RRPTRSVATVDLAAGSADPGPAIRPTSVVLASDAQAEAPLLAAAPSIGLDLPLRFVVW
                     LDDQNRTQIGHPDVRRIAARHGVAVDDPNIAQLAADADRLSRLAAGIA"
     gene            3179..3490
                     /locus_tag="VFE39_10440"
     CDS             3179..3490
                     /locus_tag="VFE39_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259057.1"
                     /translation="MIVTGYDDRQGCGYVLSTGADAADGLPGGKPVSHNGVVVQATPE
                     LAALLTDALAHRPTALLSWSGPRVPLTLDDTTLLAWLTNDVGGVLTVRGTPAPHPRAP
                     G"
     gene            3891..4655
                     /locus_tag="VFE39_10445"
     CDS             3891..4655
                     /locus_tag="VFE39_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6259058.1"
                     /translation="MTDVDAGQVGCLPLTGERTVPNVSHENYWFRRHEVVYRALLPRC
                     SGAVVLEAGCGEGYGADLLADSAAAVLALDYDPVTAAHVARRYPRVGSARANLVALPV
                     RDGGCDAVVSLQVIEHLWEQERFLRECHRVLRPGAALLLSTPNRLTFSPGRDTPLNPF
                     HTRELSGSELAGLVRGAGFVDVEILGLHHGRRLRELDARHGGAIVDAQVAVAVAGAQW
                     PPALLDDVASVTCADFELSAGDLDASLDLVALAVRP"
BASE COUNT          631 a         1800 c         1578 g          692 t
ORIGIN      
        1 gtcgcccacc acaccgaagt cggcgacctc gaagatcggc gcctcggcgt ccttgttcac
       61 cgcgatgatc gtcttcgacg tctgcatccc ggcccggtgc tggatcgccc cggagatgcc
      121 caacgcgatg tagagctgcg gagacaccgt cttgccggtc tgccccacct ggaactggtg
      181 cgggtagtag cccgagtcca ccgcagcgcg cgacgcgccg acagcggcac cgagcgagtc
      241 ggccagcgcc tcgaccacct ggaagtcgtc ggccgagccg acgccccggc caccggaaac
      301 caccacggat gcctcggtca gctccggccg gtcgccgccc acgatcggct cccggctcgt
      361 gacggtggcg acgcggccgt gtgcggccgg caccggcacg gtctcctccg cgcccgcacc
      421 cgcggacggc tcgatctcca ccgagccagg ccgcagcgtg atcaccgggt gcgcggtggt
      481 ggccttcgcc tccgcggtgt acgcaccgcc gaacaccgag tgtgtgacgc cctccggcga
      541 cacggccacg acgtcaccga gcagcccgct gttcgtgcgc accgcgaccc gcccggcgat
      601 ctccttgccg tcggccgaca ccgcgatgag caccgccgcc gggtccttcg actcgatcag
      661 cgacgccagc acccccacct ccggggtgag gaactccgtc gagtcggtct ccgcgacgta
      721 gatcttctgc gcgccgtgct ccttcagggc accgcccagc ttggcggcgg tgccggccgg
      781 gccgaccacg accgccgacg gctcacccag cgtgcgcgcc gcggtcagca gctcgaacgt
      841 actcttcttg atctcaccct cgaggtggtc gacgaggacc agcacctcgg ccatgaactt
      901 ctcctcttcc cgtcgatccc gcgtcagatg agcttctggg agaccaggaa cgccgccacc
      961 ttcgcgccgc cgtcgccctc gtcctcgatc ttctcgcccg cgctcttcgg cggcttcggc
     1021 tgcgaggacg tcaccgacgt cagcgcgttg gcgagcccga cctccgacgg gtcgatcccg
     1081 gcgtcggcca ggctgatcgt ggagaccggc ttcttcttcg ccgccatgat ccccttgaac
     1141 gacgggtaac gcggctcgtt gatcttctct cccacgctca cgaccgcggg gagctcggcg
     1201 gtcaggtgcg tgacgccgtc gtcggtctca cgccggaccg tgaccgtcga cccgtcgacg
     1261 gtgacctccc gcgcgtgcgt cagcgacggc aggccgagca cgtcggcgac catcgcgggc
     1321 actgcggcac tccgcccgtc cgacgcctcg ttgccggcca ccaccaggtc gaacccgccc
     1381 tcgacggtgc cgagcgcctt ggccagtgcc cgagcggtct gcacagcgca cgagccgtgc
     1441 agcgcctcgt ccgacagatg caccgccttg tccgcgccca tcgacagcgc cttacggatc
     1501 gcgtcggtgg cccggtcggg ccccatcgtc aggacggtga ccgtcccgtc gtgggcctcc
     1561 ttcagctgca gggccgcctc gaccgcgcgc tcgttgatct catcgagcac cgcgtcggtg
     1621 gcgtcccggt ccagggtgcc gtcggacgag ttgagcttgc gctccgagta ggtatccggc
     1681 acctgcttga ccagcacgac gatgttcatc ggacctcttc gacctcctgc cggtgcggac
     1741 gagccgcggt gggtctcacg tgggcggtct tgctcggcgc ccggttccga ctctgccacc
     1801 ctccaccacg gctcgctgcg gtgggcagcc ggatcgggca attgttaccg gtgagtatcc
     1861 ggagcggcaa acggcccgcg ggtgactctc ctcacccgag gcggaggccc cggccgcggc
     1921 cgcgatcgtg tggcagcggc ccggaccggg tatccgacaa aatgcccatc atggatcgga
     1981 tccgtagctt cctcgccggc cctcagggga ggcgtctcac cgaacagggt cgccgcatgg
     2041 ccgccgaccc gcgcaaccag gagcgggccc gcggcctgct ggcgcgcttc cggcgccgct
     2101 gaccggcgag ccgacgcgtt ccgccggacg ggggggcccc ggccccgcta gggtcgggca
     2161 ccgtctactc gcggagctgc cccaatgtcc gttctggtca gagcccaccc cagccggagg
     2221 tccggcacga tccgcacgtg gggcgcggcg atgacgctgg ccgtggtcgc ctccgcgtgt
     2281 gggaacccca cgggcggcga agtttcgctg gagccgcaca cggcaccggg ccagccggcg
     2341 ggcacgatgc tgctggcgac cggcacggac ttcgccgcca tcgtgcagcg ggtgcaggag
     2401 gccatcagcg agaccggcgg ctccgtcacc accgtggtcg atcacgccgg cgacgcacga
     2461 gcagccgggg tcgcgatacc gccctccacc ctggtcatcg ggggctcgcc tgccgctcag
     2521 ctgccgctgc tacgcatcga ccagcgggcg ggggccgacc tgccgcagcg ctatctcgtg
     2581 cgccagggct ccgacggctc ggtgagcctc accgtcgaca gcgccgacta cgtggctgcg
     2641 gtggcgggcg tgtccgaccc cggagcgcgg aatgcgctgc gtgacagcac cgccgccgtc
     2701 ctcggggagg cagtgccggg caccccggtc ccggtcccgt ccccgcttgt cggcgtgaca
     2761 ccgtcgaact atctgctgac cgtggtcggc agcgcgagcg ttcccaccac tgtcgatcga
     2821 ctccgccggg gtgccgaccg ccgcccgacc cgctccgtcg ccacggtcga cctggcggcg
     2881 ggctccgcgg accccgggcc cgcgatccgg cccacctcgg tggtgctcgc gagcgacgcc
     2941 caggccgaag caccgctgct cgccgccgcc ccgtcgatcg ggctggacct tcccctgcgc
     3001 ttcgtcgtgt ggctcgacga ccagaaccgc acccagatcg gtcatcccga cgtgcggcgc
     3061 atcgccgccc gccacggagt ggccgtcgac gacccgaaca tcgcgcagct agccgccgac
     3121 gccgaccgcc tctcgcggct cgccgccggc atcgcctaat gagcaacgga gcgagacggt
     3181 gatcgtgacc ggttacgacg accggcaggg atgcggctac gtcctcagca cgggggccga
     3241 tgccgccgat ggcctccccg gcggcaaacc cgtcagccac aacggggtgg tggtacaggc
     3301 caccccggag ctcgccgccc tgctgaccga cgcgcttgcc caccggccga cggccctgct
     3361 gagctggtcg gggccgcggg tcccgctcac gctcgacgac accacgctgc tggcctggct
     3421 caccaacgat gtcggcgggg tcctcaccgt ccggggcacg ccagcccccc acccgcgggc
     3481 tccgggttga cagagctacg gccacccgcc cactgagggt ccaggcaggc taggcccatc
     3541 tcgttgaacc ggcgaatgac ctgccgaatc gcgtcctcgt cgcctaggac cagccgagcg
     3601 atggccggca cggtgttccc gctggccgag gccaggatga ccatcgcccg ccgcagccgc
     3661 accgcctatc cactcccgcg ccgcacgatc tgttggagac gcgggccttt ctgatcggtc
     3721 agccgacgaa ccctgaccgg ttctccattc gaccagcctg accgcttggt gatcatcagt
     3781 cgcgcggtcg gcgtgttgcc ctgatgccgg ccaacgttca tggacacagc acgagcactg
     3841 gacggacgca tcacgtggtg ggaccggacc agcggtaggg taccgacgcg atgacggacg
     3901 ttgacgctgg tcaggtgggc tgcctacccc tcacgggaga gcgcacagta cccaatgtaa
     3961 gtcacgagaa ctactggttc cgccgccatg aggtggtcta ccgggcactg ctgccgcgct
     4021 gctcgggcgc ggtggtgctg gaggcagggt gcggcgaggg ctacggcgcc gacctgctgg
     4081 ccgacagcgc agccgccgtg ctggcgctgg actacgaccc cgtcaccgcc gcgcacgtgg
     4141 cacggcgcta tccgcgcgtc gggtctgcac gtgccaacct cgtggcactc cctgtgcggg
     4201 acggcggctg cgacgcggtg gtctcgctgc aggtgatcga gcacctctgg gagcaggagc
     4261 ggttcctgcg cgagtgccac cgggtgctgc gccccggcgc cgcgctgctg ctctccacgc
     4321 ccaaccgcct gacgttctcc cccggccgcg acaccccgct caacccgttc catacccggg
     4381 agctctcggg tagcgagctg gcggggctgg tccggggcgc cggattcgtc gacgtcgaga
     4441 tcctcgggtt gcaccacggc cggcggctgc gcgagctcga cgcccggcac ggcggcgcga
     4501 tcgtcgacgc acaggtcgcc gtggccgtcg cgggagcgca gtggcccccg gccctgctgg
     4561 acgacgtcgc ctcggtgacg tgtgccgact tcgagctcag tgcgggcgac ctcgacgcga
     4621 gcctggatct cgtcgccctc gccgtgcgcc cgtgagtgag cctgccggga cgttctgcct
     4681 ggtcctgcac agccacctgc c
//
LOCUS       DASRSC010000213         6867 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_897638,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000213 DASRSC010000000
VERSION     DASRSC010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_897638"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1323)
                     /locus_tag="VFE39_10450"
     CDS             complement(61..1323)
                     /locus_tag="VFE39_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259059.1"
                     /translation="MSSSSASLADYRTVLTSPGASAPALASAVGRLPIAMFGLATLLY
                     VQRASGSFGAAGLVSAGILAGESLGAVVQGRVMDRRGPTRLLLLVAVLFGLAVTLLTT
                     AVEADQPLPVLVALALVAGLVQPALAGASRALWTDLVPRGPLRDAAFGYEAISLEVFF
                     ILGPAIAAFLVLAPWPGTGLVVAAAAMVAGSVGFALTPAVRARPSVLTHPGPSGSIAP
                     SVGLLGALSRPGMRTVALASLGFGLVVGGVEVGVPAVTAAAGSAAAGGLLLSAWSVTS
                     VLAGVLYGLQPWPRPLHLRLPVLLGAFAVLVAAMALTGPLGSLPVLVVAMLMAGALIT
                     PQATAHSMAVDSAAPTGTAAEAFGWVVTAAALGLAAGQSVAGIVIEAAGPPAAFLASG
                     SAGVVLAVVLWLRRGTLALMATPAPVAA"
     gene            complement(1434..2108)
                     /locus_tag="VFE39_10455"
     CDS             complement(1434..2108)
                     /locus_tag="VFE39_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HET6259060.1"
                     /translation="MDEVLIRAGIFQGVEPHAADALAQALEPAEFPRGHVIFAEGEPG
                     DRLYIVGSGKVKIGRKSPDGRENLLMVAGPSDMFGELSIFDPGPRTSSATAVTEVRTY
                     TMDRGALREWIAKRPEIAEQLLRVLARRLRRTNNMLADLIFTDVPGRVAKSLLQLARQ
                     FGSQESGLLRVTHDLTQEEIAQLVGASRETVNKALADFAHRGWLRLEGKSVLILEPER
                     LARRAR"
     gene            complement(2287..2538)
                     /locus_tag="VFE39_10460"
     CDS             complement(2287..2538)
                     /locus_tag="VFE39_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259061.1"
                     /translation="MTAWLSVLVPLLVMFFALGMERVESKMREATLRPEELDELLERP
                     RPDEIRALFRQGTGRALELFRLRNRAPRGRRSAKHGKAA"
     gene            2790..3524
                     /gene="nth"
                     /locus_tag="VFE39_10465"
     CDS             2790..3524
                     /gene="nth"
                     /locus_tag="VFE39_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010307876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET6259062.1"
                     /translation="MLRTLAATHPDAHCELDFRTPLELAVATILSAQSTDKLVNSVTP
                     ALFERYPTALDYAKADRNELETMIHSTGFFRNKASSLIGLGAAVVEKHDGVLPHTLAE
                     LVALPGIGRKTANVILGNAFDVPGITVDTHFGRLVRRWGWTQEEDPVKVEHAVGALVP
                     KRDWTIVSHQVIFHGRRVCHARKPACGACTLSNDCPAYGTGPIDPIAAAALVKGPSRP
                     HLLAMAGIPDDAAPAVEPAGEDQDAP"
     gene            3580..4227
                     /locus_tag="VFE39_10470"
     CDS             3580..4227
                     /locus_tag="VFE39_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010307878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6259063.1"
                     /translation="MSREQVRVRRSELVTTLVVVLLAAAAVFALWPRSAPERSDPAGA
                     RTPTAVAVPDAELAPLRAAARAAPCPSGAGAQAAGPLAGITVPCLGAPGLVDVGAALA
                     GRPALINVWASWCAPCRDELPALAAYAARPDAVPVLGIDARDDPRAALSLLRDVDVTL
                     PSVTDPDGTLRAALDIPPALPVSYVVRADGSVARVDPPVPFQSADDVAAAVKRLS"
     gene            4241..4933
                     /locus_tag="VFE39_10475"
     CDS             4241..4933
                     /locus_tag="VFE39_10475"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HET6259064.1"
                     /translation="MTHLQPERAPAWLDSLLRRVGGVDAATLSHHPIPPPEGGRRAAV
                     LVLFGEDAVHGPDVLLVERASTLRDHAGQVAFPGGGADPEDPDAVATALREAEEETGV
                     EPGGVVPLAVLPELFIPPSGFVVTPVVGYWERPVAVHAVDPGETAAVVRVPLAALADP
                     ANRLRVRHPSGTTGPAFAVAGLLVWGFTGALLSALLDLGGWASPWDITRVDDLSAAWS
                     AARASRQEVAGS"
     gene            5153..6349
                     /locus_tag="VFE39_10480"
     CDS             5153..6349
                     /locus_tag="VFE39_10480"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarP family serine protease"
                     /protein_id="HET6259065.1"
                     /translation="MSWVDGVVLGLALIAAVSGWRHGMAVALLSFVGVLTGAVLGVRL
                     APLLAAGVQSPTTRVVVSVVVVVLLVALGETTGVFFGRRIRDRITGQRSLQVDSTLGS
                     VLQAITVVIAAWLVALPLASASFPGLASGVRGSEVLRAVDSVMPAGAKALPAELRQLL
                     NNSGFPDVLSPFAQTPITEVGPPDPALAQSPMIADVSSSVLKIRGRAPSCARQLEGSG
                     FVIGPQLVLTNAHVVAGTDETGVEVAGKRGRTTELSAEVVLYDPEVDVAVLRVPDLVA
                     KPLPFDSQPAKPGDDAVVLGYPLDGPYTATAAKIRSMINLKGPDIYDDGEVTREVYTV
                     RAVVRSGNSGGPLISPTGQVIGVVFGAALDDQETGFVLTVQQVASVVQRAPGLRTEVT
                     TGSCAA"
     gene            complement(6362..>6867)
                     /locus_tag="VFE39_10485"
     CDS             complement(6362..>6867)
                     /locus_tag="VFE39_10485"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HET6259066.1"
                     /translation="TTVLGILKAYTTRVGSGPFPTELLDAMGEHLRKAGGEVGVTTGR
                     PRRCGWFDAVIGRYAARVNGITDFFLTKLDVLSGLEKVPICVGYEVDGRRIDEMPMTQ
                     TDVHHAVPVYEELPGWFEDLSACRTFDELPAAARAYVHRIEELTGAPVSAIGVGPGRD
                     QTITRDV"
BASE COUNT          987 a         2526 c         2438 g          916 t
ORIGIN      
        1 gctgccgagt ggtcggtgcg agcgcagctg gactacctgc gcgcctcggc ctgagagtcg
       61 ctatgcggcc accggggccg gggtcgccat gagggcgagg gtgccgcggc gcagccacag
      121 gaccacggcc agcaccacgc cggccgagcc actcgccagg aaggccgcag gcgggccggc
      181 tgcctcgatc acgatccccg ccaccgactg tcccgcggcg agcccgagcg cggcggcggt
      241 gaccacccag ccgaacgcct ccgccgccgt ccccgtggga gcggcgctgt cgacggccat
      301 cgagtgcgcc gtggcctgcg gcgtgatgag cgccccggcc atgagcatcg ccaccacgag
      361 caccggcagc gaaccgagcg gccccgtcag cgccatcgcc gcgacgagca cagcgaacgc
      421 tccgagcagc accggcagcc gcagatgcaa cgggcggggc cacggctgca gcccgtacag
      481 cacacctgcg agcaccgacg tgaccgacca cgctgacagc aacagaccgc ccgccgcggc
      541 cgatccggcc gcagccgtca cggcgggcac accgacctcc accccgccga cgacgaggcc
      601 gaaccccaac gacgcgagcg cgacggtgcg cataccgggc cgggacagcg cgccgagcag
      661 cccgaccgac ggggcaatgg aaccggacgg cccggggtgc gtcagcacgc tgggtcgggc
      721 ccggaccgcg ggggtgaggg cgaacccgac cgagcccgcg accatggccg cggcggccac
      781 caccaggccg gtgccgggcc acggggcgag cacgaggaac gcggcgatcg ccggaccgag
      841 gatgaagaag acctcgagac tgatcgcctc gtagccgaat gcggcgtccc gcagcggtcc
      901 gcgcggcacg aggtcggtcc acagcgctct ggacgccccg gccagggcgg gctgcacgag
      961 cccggcgacc aacgccaacg ccacgagcac cggcagcggc tggtctgctt cgaccgcggt
     1021 cgtgagcagg gtaaccgcca acccgaacag caccgcgacc aacagcagca gccgggtagg
     1081 cccgcgccgg tccatcaccc ggccctggac gaccgcgccg agcgactccc ccgccaggat
     1141 gcccgcggac acgagacctg cggcaccgaa cgagccggag gcccgctgga cgtagagcag
     1201 ggtagccagg ccgaacatcg cgatcggcag gcggccgacc gcggaggcga gcgccggggc
     1261 ggacgccccc ggcgacgtca ggaccgtgcg gtagtcggcg agcgaagcgg acgaggacga
     1321 cacgtgcgcc agcgtgacac attctggtac gtccgtacca tttctttacc gcgagcgcgt
     1381 cggcgtgcac tcccgtgcga tagccgggaa tgtggtccgg gaactccggc gcgctatcgc
     1441 gcccggcggg cgagccgctc tggttcgagg atgagcacgc tcttgccctc cagccgcagc
     1501 caaccgcggt gggcgaagtc ggccagcgcc ttgttgaccg tctcccggct cgcgccgacg
     1561 agctgcgcga tctcctcctg cgtcaggtcg tgggtgacgc gcagcagccc cgactcctgc
     1621 gagccgaact ggcgcgcgag ttgcaacagc gacttcgcca cccggcccgg cacgtcggtg
     1681 aagatcaggt cggcgagcat gttgttggtg cgccggagcc gccgcgcgag cactctgagc
     1741 agctgctcgg cgatctcggg ccgcttcgcg atccactccc gcagcgcgcc gcggtccatc
     1801 gtgtacgtgc ggacctcggt gaccgcggtg gccgacgacg tacgcggccc cgggtcgaag
     1861 atggacagct caccgaacat gtcggacggc ccggccacca tcaacaggtt ctcgcgaccg
     1921 tccggcgact tgcggccgat cttgaccttg ccgctgccga cgatgtagag ccggtccccc
     1981 ggctcgccct ccgcgaagat gacatgccca cgggggaact ccgcaggctc gagagcctgc
     2041 gccaaggcgt cagccgcgtg cggctcgact ccctggaaga tccctgcgcg gatcagaacc
     2101 tcgtccactg caccgcccct cgtccccagc cgctttccgc ggcggaccac tggccggcca
     2161 gtctaggggg tgcgtcgacc gtccgtgacg ccgcaaccga ccttcacccg cagcccggag
     2221 cggtttcgtc acgaataatc ccagttcagt acggttcgac cggtctcaag acgccccatg
     2281 cgggcgtcat gcggccttgc cgtgcttggc ggaccgacgc ccgcgcggag cgcggttgcg
     2341 cagtcggaag agttcgagcg cccggccggt gccctggcgg aacagggccc ggatctcgtc
     2401 ggggcgcggg cgttcgagga gctcgtcgag ctcctcaggg cggagtgtgg cctcccgcat
     2461 cttggattcc acccgctcca taccgagcgc gaagaacatc accagcagcg gcacgaggac
     2521 ggacaaccat gcagtcatga cggggggatc gtctcgcacg agtgacaggc gacgaccggc
     2581 aggggtgagc atgttggcga gtcgatatcg tttcgtaaca aagccggtca cgcaccgtca
     2641 ccgccggtct gcgccgggcg acttcgccgg gcgtcggcgt cgcgccgtac gctcgaagcg
     2701 tgaccaccgc ttcgcggaac gcggcgcgcc tcgccaaacg ggtggccgac ggggagccgt
     2761 ccctcggacg ggcccgacgg gtcggccgga tgctgcgcac cctcgccgcc acccatcccg
     2821 acgcccactg cgagctcgac ttccgcaccc cgctggagct cgccgtcgcc acgatcctgt
     2881 cagcgcagag caccgacaag ctcgtcaact cggtcacgcc cgctctgttc gagcgctacc
     2941 ccaccgcgct cgactacgcg aaggccgacc gcaacgagct cgagacgatg atccacagca
     3001 ccggcttctt ccggaacaaa gcctcctcgc tcatcgggtt gggcgccgct gtcgtcgaga
     3061 agcacgacgg cgtgctcccg cacacgctcg ccgagctggt cgccctgccc ggcatcggcc
     3121 gcaagacggc caacgtgatc ctcggcaacg ccttcgatgt cccgggcatc accgtcgaca
     3181 cgcatttcgg acggctcgtc cggcgctggg gatggaccca ggaggaggac ccggtcaagg
     3241 tcgagcacgc ggtgggagcg ctcgtgccca agcgcgactg gacgatcgtg tcgcaccagg
     3301 tgatctttca cggccgccgc gtctgccacg cccgcaagcc cgcgtgcggc gcctgcacgc
     3361 tgagcaacga ctgccccgcc tacggcacgg gccccatcga tccgatcgcg gccgccgcgc
     3421 tcgtcaaggg cccgtcgcgg ccgcatctgc tcgccatggc cggtatcccc gacgacgccg
     3481 cccccgcggt cgaacccgcc ggcgaggacc aggacgcgcc atgacgacgg gccccgccca
     3541 ccacgagcac gtccacagat gctcccgaga ggcgtgccgg tgagccgcga gcaggtcagg
     3601 gtgcgccgat cggagcttgt caccaccctg gtcgtggtgc tgctggcagc ggcggccgtc
     3661 ttcgccctgt ggccgcgcag cgcaccggag cgcagcgacc cggcgggcgc tcggacgccc
     3721 acggcggtag cggtgcccga cgccgagctc gccccgctgc gtgccgccgc gcgggcggca
     3781 ccatgcccgt cgggcgccgg cgcccaagcg gccggaccgc tcgccgggat caccgttccg
     3841 tgcctcggcg cgcccggtct cgtcgacgtc ggcgccgcgc tcgccggccg gcccgctctg
     3901 atcaacgtct gggcctcgtg gtgcgcgccg tgccgcgatg agctgcctgc actggccgct
     3961 tacgccgccc gaccggatgc agtaccggtg ctgggcatcg acgcccgcga cgatccgcgg
     4021 gccgcgctgt cactgctccg cgacgtcgac gtcacgctgc cgtcggtcac cgatccggac
     4081 ggcacgctgc gcgccgcgct ggacatccct cccgcgctgc ccgtgtccta cgtcgtgcgt
     4141 gccgatggca gcgtcgcccg ggtcgacccg cccgtgccgt tccagagcgc cgacgacgtc
     4201 gccgccgccg taaagcggtt gtcgtgaggg tgggacggcc gtgacgcacc tgcagcccga
     4261 gcgggcgcct gcctggctgg attcgttgct gcggcgtgtc ggcggcgtcg atgcggccac
     4321 gctgagccac caccccatcc ctcctccgga ggggggccgg cgggccgctg tgctcgtgct
     4381 cttcggcgag gacgccgtac acggtcccga cgtgctgctc gtggaacggg ccagcaccct
     4441 gcgcgatcac gccggtcagg tggcgttccc cggtgggggt gccgacccgg aggaccccga
     4501 cgccgtcgcc accgctctgc gggaggccga ggaggagacc ggggtggagc cgggcggtgt
     4561 ggtgccgctg gcagtgctgc ccgagctgtt catcccgccg tcgggcttcg tcgtcacccc
     4621 tgtggtgggg tactgggagc gtccggtcgc cgtgcatgcg gtcgacccgg gcgagaccgc
     4681 cgccgtcgtg cgcgtgccac tcgccgcgct ggctgacccc gccaaccggt tacgggtccg
     4741 ccacccgtct ggtaccaccg ggcccgcatt cgccgtagcg gggctgctcg tgtggggttt
     4801 cacgggggcc ttattgtccg cgctgttgga tctagggggc tgggcgagcc cctgggacat
     4861 cacccgggtg gacgacctca gcgcagcgtg gtccgcggca cgggccagtc ggcaggaggt
     4921 cgcaggatca tgagcgcagg gctccggcgg gccgacccgg gcgagatgtg cgcacaacgg
     4981 gcggcgcgtt gagcgagcgc gcgagcgaat catcgacgca gcgcccgcgc gccgagacat
     5041 ctcacgatgg gccggccgag cgcagcgagg gcgagccagt gagcgagctt gcgagcgaat
     5101 catcgacaca gcgcctgcgc gaagcgccga ccgagcgcag cgagggcgag cgatgagctg
     5161 ggtcgacggg gtggtgctgg ggctggcgct gatcgcggcg gtgtccggtt ggcggcacgg
     5221 gatggccgtg gcgctgttgt cgttcgtcgg cgtgctcacc ggggcggtgc tcggggtgcg
     5281 gctggcaccg ttgctggccg ccggcgttca gtcgcccacc acgcgggtcg tcgtcagcgt
     5341 cgtcgtggtc gtgctgctgg tcgccctcgg cgagaccacc ggcgttttct tcgggaggcg
     5401 catccgcgac cggatcaccg gccaacgctc actgcaggtc gactccacgc tggggtcggt
     5461 gctgcaggcc atcaccgtcg tcattgctgc gtggctcgtc gcactcccgc tggcctcggc
     5521 cagcttcccc gggctcgcct cgggcgtccg cgggtcggag gtgctccggg ccgtcgactc
     5581 ggtgatgccg gcaggcgcca aggcgctgcc tgccgagctg cgtcagctcc tgaacaactc
     5641 cggcttcccc gacgtgctca gcccgttcgc gcagaccccg atcaccgagg tcggtccgcc
     5701 cgaccccgcg ctcgcgcaga gtcccatgat cgctgacgtg agcagcagcg tgctcaagat
     5761 ccgcggtcgc gcgccgtcgt gcgcgcggca gctggagggc agcggtttcg tgatcgggcc
     5821 ccagctcgtg ctgaccaacg cccacgtcgt ggcgggcacg gacgagaccg gcgtcgaggt
     5881 cgccgggaag cgagggcgaa cgaccgagct cagcgccgag gtggtgctct acgaccccga
     5941 ggtcgacgtg gcggtgctcc gggtgcccga cctggttgca aagccgctgc cgttcgattc
     6001 tcagccggcg aagccgggcg acgacgctgt cgtgctcggc tacccgctcg acggtcccta
     6061 caccgccacg gcggccaaaa tccgatcgat gatcaacctg aagggcccgg acatctacga
     6121 cgacggcgag gtgactcggg aggtctacac cgtgcgggcc gtggtccggt ccggcaactc
     6181 cggcggcccg ctcatctcac cgaccggcca ggtgatcggg gtcgtcttcg gcgcggctct
     6241 ggacgaccag gagaccgggt tcgtcctcac cgtccagcag gtggcctccg tggtgcaacg
     6301 ggcgccgggc ctgcgcaccg aggtgaccac gggtagctgc gccgcgtgag gcgggccggg
     6361 ctcagacgtc gcgggtgatc gtctggtcgc ggccgggtcc cacaccgatc gcgctcacgg
     6421 gcgcacccgt cagctcctcg atgcggtgga cgtaggcgcg cgccgcagcc gggagctcgt
     6481 cgaacgtccg gcacgccgac aggtcctcga accagcccgg cagctcctcg tacacgggca
     6541 cggcatggtg cacgtcggtc tgtgtcatcg gcatctcgtc gatgcgcctg ccgtcgacct
     6601 cgtagcccac gcagatcggc accttctcca gtcccgacag cacgtcgagc ttggtgagga
     6661 agaagtcggt gatcccgttg acgcgggcgg cgtagcggcc gatcaccgcg tcgaaccagc
     6721 cgcaacggcg cggccgcccg gtggtgaccc ccacctcgcc accggccttg cgcaggtgct
     6781 cacccatcgc gtccagcagc tcggtgggga acgggccgga gccgacgcgg gtggtgtagg
     6841 ccttcaggat tcccagcacg gtggtga
//
LOCUS       DASRSC010000214        12149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1715945,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000214 DASRSC010000000
VERSION     DASRSC010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12149
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1715945"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="VFE39_10490"
     CDS             <1..1407
                     /locus_tag="VFE39_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine dehydrogenase"
                     /protein_id="HET6259067.1"
                     /translation="TSLMRWLRRLADADLALDRTMIPLGSCTMKLNAAVEMEPITWPE
                     FADLHPFVPEADAEGTLALIADLERWLAELTGYAAVSLQPNAGSQGEFAGLLAIAAYH
                     RSRGEAHRDVCLIPASAHGTNAASAVMAGMRVVVVATSATGDVDLDDLHAKIAEHGPA
                     LAALMITYPSTHGVFEARVREVCDAVHEAGGQVYVDGANLNALLGLARPGAFGGDVSH
                     LNLHKTFCIPHGGGGPGVGPVAVAEHLAPFLPGRGEVGAVSAAPHGSPGVLPISWAYV
                     RLMGTDGLRRATLTAITAANYVAKRLAEHYPVLYAGERGHVAHECILDLRGITSDTGV
                     TVDDVAKRLADYGLHAPTMSFPVAGTLMVEPTESEDLAEIDRFVNAMIGIRAEIDRVA
                     AGEWPVTDNPLRGAPHTAASLIDKWDHPYPRELAAYPTGVTPGVHDRKVWPPVRRIDG
                     AHGDRNLVCSCPPVEAYS"
     regulatory      1777..1950
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            2255..3886
                     /locus_tag="VFE39_10495"
     CDS             2255..3886
                     /locus_tag="VFE39_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HET6259068.1"
                     /translation="MTTTMGERRSAVLDDAHVGDIRGALGTIRLDDTGPRRRLSAKLK
                     TLLAIIGPGLIVMVGDNDAGAFATYGQAGQNYGTKLLWTLLLLVPVLFVNQEMVLRLG
                     AVTGVGHARLILARFGRFWGAFSVIDLLILNALTLVTEFVGITMAVGYLGLPKIPAVI
                     LAGGVIVAAAFTGSFRRFERIAVAFCLGSLLLIPVYVLAHPAVSQMAHDLVVPSLPAG
                     SGGMATLMLLIIGIVGTTVAPWQLFFQQSYIIDKRITPRFMRYEKVDLWIGIGVVMVG
                     AAATMGFTAVIFAGTQGFGNFTSSTGIAQGLAAYAGKAAGALFAVALLDASIIGAFAV
                     SLSSAYAIGDVFGVKHSLHRGVKQAKGFYLVYAGLIGLAAALVLIPGSPLGLLTAGVQ
                     TLAGVLLPSASVFLLLLCNDRQVLGPWVNGRATNLFTSIVVGVLVTLSIILTSAVLFP
                     DIAAGQIVTIIQVCAGVGVLATGYALLRRFRGGSAAGRIDRSDQQDWRMPPLDELTKP
                     VMSTGRKIGMGALRLYLAAAMILVIIKIAQTVLGH"
     gene            complement(4148..5035)
                     /locus_tag="VFE39_10500"
     CDS             complement(4148..5035)
                     /locus_tag="VFE39_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyladenine DNA glycosylase"
                     /protein_id="HET6259069.1"
                     /translation="MTTELTSAAPVVLEEPVWRARAARHRARVGRWTGLHRERRRRGE
                     SHPVMDFLFTYYSLRPARLELWHPGPRALLRGGDELLAVPGHVRDGDCVRLDVDALPP
                     RITGTAAFVRDLLLATASRPARLSCFGLHEWAMVYGSDAPRHGAVPLRLGAAGTDAVV
                     ESLSVHCTHHDAFRFFTAAARPLNAISPERADQIANEQPGCLHATMDLYKWAYKLAPA
                     TPSELLADCFALAVEVRVLDMRASPYDLADLGYSPVRIETPSGRAEYARAQAAFGERA
                     APLRDRLIALCRVLAPVEA"
     gene            complement(5067..5486)
                     /locus_tag="VFE39_10505"
     CDS             complement(5067..5486)
                     /locus_tag="VFE39_10505"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019819086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET6259070.1"
                     /translation="MTTMTSIFTPAELAYLRGQRLARLATIGPDGAPQVRPVGFRVDA
                     ENGTVDIPGLQNPSTQKWRNVVRDGRVALVVDDVVPEPWRPRALEIRGTAEALPDAMP
                     AGFPGVRPGVIRIHPERILVFGLDDPDSPGARRVRPA"
     gene            complement(5520..6572)
                     /locus_tag="VFE39_10510"
     CDS             complement(5520..6572)
                     /locus_tag="VFE39_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET6259071.1"
                     /translation="MGNDLLLLLAAVALLGANAFFVGAEFALISARRDRLEAMAVSGT
                     PAARTVLRAGADLSRMLAASQLGITISSLLLGRLGEPAVAHLIERPLQWAGLPEALLH
                     PISFTVSLAIVVVAHILLGEMVPKNIAIAGPERTAIWLVPPFLVFTTVMRPLIELFNV
                     MANGVLRLVGVESRDELETSFTSGELADLIAESGREGLLDDHESRRLTRTLSSAEATV
                     ADVLVPSDELVTLPANPTVENVAQTVADTGFSRFPVPAPDGKLIGYLHVKDILDLVDD
                     PDTAVPPERVRGLPEVPVSFRLDEALAVLRRSRAHLGRVVNGRGETVGVVAMEDLVEH
                     YVGEVRDATHAPRSQA"
     gene            complement(6565..7920)
                     /locus_tag="VFE39_10515"
     CDS             complement(6565..7920)
                     /locus_tag="VFE39_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET6259072.1"
                     /translation="MSVLMSVLGLIAVAALTLGTAISVASEFALTALERSQVDAHVAE
                     VGDRRARAVQRAHRGLSFQLSGSQLGITVTTLVTGYIAEPAIASLFRPGLTAIGLSDG
                     LAAGIATVLSLLLATTLSMVFGELVPKNLAIADPLRTARAVVWLQSGFARAFRWLITG
                     LNSAANAVVRRLGVEPAEELRSARSPQELSSLVRTSAEHGTLDAGTATLLDRSLRFTD
                     RVAEDLMTPRVRVESLDADDTVADLVMLARRSGFSRFPVHDRDPDAVLGVVHVKQAFG
                     VPAAKRERAVLRDMVQPVPTVPGSLDGDELLTRLRGSGLQLAVVVDEYGGTAGIVTLE
                     DLIEEIVGDVRDEHDRAEQAQVRPLGRGSWLVSGLLRGDEVADATGFEMPPGDYETLA
                     GMVLARLGRIPDVGEDVVVDGWRLTVMRRDRNRIAELRVACADTGGAASTAKREEATR
                     G"
     gene            8288..9250
                     /gene="coaA"
                     /locus_tag="VFE39_10520"
     CDS             8288..9250
                     /gene="coaA"
                     /locus_tag="VFE39_10520"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET6259073.1"
                     /translation="MDGSRNGAREASSPFVDFDRDAWAKLGENTPLPLTAEELDQVRS
                     LGDEVDLDEVREVYLPLSRLLTMHVQEGGRLYRAYRTFLGAKTARTPFVIGIAGSVSV
                     GKSTTARLMRLLLARWPQHPRVELVTTDGFLHANAELERRGLMTRKGFPESYDRRALL
                     RFVTEVKAGRAEVTAPVYSHLVYDIVPDERLTVHRPDILLLEGLNVLQPAQPAREGGA
                     ALAVSDFIDFSVYVDAATDDIRRWYVERFLRLRETAFRDPESYFRRYAALDEAGAIAR
                     AESIWAEINGPNLERNIKPTRGRASLVLRKGPKHNITGIRLRKV"
     gene            9291..9740
                     /locus_tag="VFE39_10525"
     CDS             9291..9740
                     /locus_tag="VFE39_10525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6259074.1"
                     /translation="MTSAQRVDTDEQTDLAARLRLVVGRLHRRIRIDGRESIPPLQLS
                     ALATVEQHGPLRLSELARREAVTAPTMSRVLAALDDQGLVIRTPDPSDARGVRIVLSE
                     EGAARLAEVRSHRTALVARRLARLDADQRQILEAAMPALEALLVDDD"
     gene            complement(9760..10554)
                     /locus_tag="VFE39_10530"
     CDS             complement(9760..10554)
                     /locus_tag="VFE39_10530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HET6259075.1"
                     /translation="MGLTRSDAPLSAHAPKTVNYRIDGPAHKLLMHPFPRRVRAEFAG
                     RTVLDTRKGVLLHETALPPRLYVPETDIDPSAFVPSDHRTHCPFKGDATYRSVQVGDR
                     VVENALWAYPDPTAEAPWLAGYASLYWEAADAWFDEDEEVQALTDPYHRVDIRRSSRH
                     VQVLAGDTVVAESNAPLMLAETGLPLRWYLSPDDVQVPLEPTATSTHCPYKGDASYWT
                     ARLPDGRELTDAVWGYPEPLPESARIGGRVSFLHDGLRVLVDGEQV"
     gene            complement(10608..>12149)
                     /gene="asnB"
                     /locus_tag="VFE39_10535"
     CDS             complement(10608..>12149)
                     /gene="asnB"
                     /locus_tag="VFE39_10535"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HET6259076.1"
                     /translation="VLHAHREWGRRDPRDAVRELNGMFAYALWDPLARELVLVRDRLG
                     VKPLYYYPTPHGVLFGSEPKAVLANALAARVVDADGLRRALTFSANPGNSVFRGLREV
                     PPGHVVRVTREGIRELPYWRLEDTGHTDDVPTTVRHVRELLEDTAQRQLVADVPLCTL
                     LSGGLDSSALTALAARHTTDRVRSFAVDFTGYTENFSADDVRGTPDGPYVHEVAEFVG
                     TVHTDITLSTDELMEPGARAATLHARDLPITTGDLDTSLYLLFRAVRQHSTVALSGES
                     ADEVFGGYRDFHDADTVAADTFPWMAGRMLNTDMTRPGLLDQALAKQLDLPGYVEAQY
                     RTALAEVPGLTGPAAADPHERRMREICYLYLTRFLPVLLDRKDRMSMAVGLEVRVPFC
                     DHRLVEYVFGTPWSHKTFDGREKSLLRAATADLLPDSVVQRVKSPYPSTQDAGYERML
                     RAQLAALLERPDSPVAQLLDPVAVRERLAEPVNDQSGMGARVRLEQILTLDAWFTDYG
                     VTLDL"
BASE COUNT         1802 a         4139 c         4390 g         1818 t
ORIGIN      
        1 acgtcgttga tgcgctggct ccgccgcctc gccgacgccg acctggcgct ggaccgcacg
       61 atgatcccgc tcgggtcgtg cacgatgaag ctgaacgcgg ccgtcgagat ggagccgatc
      121 acctggcccg agttcgccga cttgcacccg ttcgttcccg aggccgacgc cgagggcacg
      181 ctcgcgctga tcgccgacct cgagcgctgg ctcgccgagc tgaccggcta cgccgccgtg
      241 tcgctgcagc ccaacgccgg gagccagggc gagttcgcgg gtctgctcgc gatcgctgcc
      301 taccaccgca gccggggcga ggcgcaccgc gatgtctgcc tgatcccggc gagcgcccac
      361 gggaccaacg cggcctcggc ggtgatggcc gggatgcgcg tggtcgtggt ggccacctct
      421 gccaccggcg acgtcgacct cgacgacctg cacgccaaga tcgccgagca cgggccggca
      481 ctggccgcgc tcatgatcac ctacccgtcg acgcacgggg tcttcgaggc gcgggtccgc
      541 gaggtgtgcg acgccgtgca cgaggcgggc ggccaggtct acgtcgacgg tgcgaacctc
      601 aacgcgctcc tcgggctggc ccggcccggc gcgttcggcg gcgacgtcag ccacctcaac
      661 ctgcacaaga cgttctgcat cccgcacggg ggcggcggcc cgggagtcgg gccggtggcg
      721 gtggccgagc acctggcgcc gttcctgccg gggcggggcg aggtcggcgc ggtctcggcg
      781 gcaccgcacg gtagcccggg tgtgctgccg atctcgtggg cctatgtgcg gctgatgggg
      841 accgacgggc tgcgccgggc cactctcacg gccatcaccg cggccaacta cgtcgcgaag
      901 cgcctcgccg agcactaccc ggtgctctac gcgggggagc gggggcacgt cgcccacgag
      961 tgcatcctcg acctgcgcgg gattacgagc gacacggggg tcaccgtcga cgacgtcgcg
     1021 aagcgacttg ccgactacgg cctgcacgcg ccgaccatgt cgttcccggt ggccggcacc
     1081 ctcatggtgg agcccaccga gagcgaggat ctcgccgaga tcgaccggtt cgtgaacgcg
     1141 atgatcggga tcagggccga gatcgaccgc gtggcagcgg gggagtggcc ggtcacggac
     1201 aaccccctgc gtggcgcccc ccacacggcc gccagcctga tcgacaagtg ggaccacccg
     1261 tacccgcgcg agctggccgc ctacccgacc ggtgtcacgc cgggcgtcca cgaccgcaag
     1321 gtgtggccgc cggtacgccg gatcgacggc gcccatggcg accgtaacct cgtctgcagc
     1381 tgccccccgg tggaggccta ctcctgacgc agagcgccgc ggatcggcga tccgcggcgc
     1441 ttcctcggag ggcaggaccc ggcccaccat gtcaatggtt cattgacgcg gacgtcccgc
     1501 caatcacaga ttgacgaccc cgggctgcct tccggcatag ccgcaaaccc aggcgtacaa
     1561 ccggaccgga agtcggcagg ttgcactgtt tctgctgtgg cgcgggctgg tcaggctcac
     1621 gttcctgcgt cggtcgacac gctcaacctc tggacctgga aaacggcttc ctatttccag
     1681 gtaaatggac ggtttcacgg gggagacctc gggcacactg gccgtcttca cgcttgtgcg
     1741 cacgatgttc ggggacctat cgtgaccgag gtttgactgg cacctcgtta ggtgaggcgt
     1801 ctgttcggac ataagccact gatctgacga cgtcgagaga cgcccaaggt taggacaggt
     1861 ttccccgggc taaggggtca cccgaagtgg cttccccggg agcgatacgg ggatacgccg
     1921 gacagtgcca aagctcttac gagatgtagg tgcaccttcc ctcactgctg atcggcggct
     1981 gcgcctgaaa tgtgtgacac cggtggtcga ccagtgtccc ggccggatgg aggtgagggc
     2041 aatgcagggc agtagtccgg gtcgaagtcg acccccgcat ccgcatgaca tgccctcgtc
     2101 ttcgttctcc ggcatccggt agttcccctg cagcatcggt tggtcgctgc ccacgtcgtc
     2161 cggcctgttg ccgggaggtg gggttgcgcc ggccaggtca ggtggcgccg cgtgccccgt
     2221 cctgccccgg tccggcggca gaggggttgt ttccatgacc acgaccatgg gggagcgccg
     2281 tagtgcggtc ctcgacgacg cgcacgtcgg cgacatccga ggcgcactgg gcaccatccg
     2341 actggatgac accggtcccc gacggcggct gtcagcgaag ctgaagacct tgctggcgat
     2401 catcggcccc gggttgatcg tcatggtcgg cgacaacgac gcgggcgcgt tcgccaccta
     2461 cgggcaggcc gggcagaact acggcacgaa gttgttgtgg acactgctgc tgctggtgcc
     2521 agtgttgttc gtgaaccagg agatggtgct gcggctgggc gcggtgaccg gcgtcgggca
     2581 cgcccgcttg atcctggcgc ggttcggccg cttctggggc gcgttcagcg tgatcgacct
     2641 gctcatcctc aacgcgctga cgctggtgac ggagtttgtc gggatcacca tggcggtcgg
     2701 ctatctggga ctgccgaaga tccccgcggt catcctggcc ggcggggtga tcgtcgccgc
     2761 ggcgttcacc gggtcgttcc gccggttcga gcggatcgca gtggccttct gcctcgggtc
     2821 gctgctgctc atcccggtct atgtgctggc gcacccggct gtctcgcaga tggcgcatga
     2881 cctcgtggtc ccgtcgctgc cggccggctc gggtggaatg gccacgctga tgttgctgat
     2941 catcggcatc gtgggcacca cggtcgcccc gtggcagttg ttcttccagc agtcctacat
     3001 catcgacaaa cggatcaccc cccggttcat gcgctacgag aaggttgacc tgtggatcgg
     3061 catcggggtg gtgatggtcg gcgcggcggc aacgatgggc ttcacggcgg tgatcttcgc
     3121 cggcacgcag gggttcggga atttcaccag cagcacgggc atcgcccagg gactggccgc
     3181 gtacgcgggg aaggcggccg gtgcgctgtt cgcggtcgcg ctgctggacg cgtcgatcat
     3241 cggcgcgttc gcggtgtccc tgtccagcgc ctatgccatc ggtgacgtgt tcggcgtgaa
     3301 gcactcactg caccgggggg tgaagcaggc caaaggtttc tacctcgtct acgcggggtt
     3361 gatcgggctc gcagcggcgc ttgtgctcat tcccggctcc ccgctgggcc tgctgacggc
     3421 aggcgtgcag accctcgccg gtgtgctgct gccctccgca tcggtgttcc tgctactgct
     3481 gtgcaacgac cggcaggtgc tcggtccgtg ggtcaacggc cgggcgacca acctgttcac
     3541 gtccatcgtg gtcggtgtcc tcgtcacctt gtccatcatc ctgacctcgg cggtgctgtt
     3601 ccccgacatc gctgccggcc agatcgtcac gatcatccaa gtctgcgcgg gtgtcggggt
     3661 gctggccacg ggctacgcac tcctccgccg ctttcggggc ggctcggcgg cgggtcgcat
     3721 cgaccgaagc gatcagcagg actggcggat gccgccactg gacgagctga ccaagccggt
     3781 catgtccacc ggacgcaaga tcggaatggg tgcgttgcgc ctgtacctgg ccgccgcgat
     3841 gatcctggtc atcatcaaga tcgcccaaac ggtgctcggc cactgacata tgcgccctgc
     3901 agcacacgcc gcaggccgat cgtcatcgaa tccacggagg agcgggtccc cgtcctcgcg
     3961 ggcgggtgga ccccctcctg cccacccgcc cgcggtgatg agaggcgggg tgtggtcgcc
     4021 cagatacatc aacttctggg cgctgccctg ttgtcaatga aacattgacg cctaccgatc
     4081 gtcgatcaaa cattgaccac gacgacggca ccggccgaag aaccggcaca acggagcgac
     4141 ctgggcttca ggcctcaacc ggggcgagga cccgacagag cgcgatcagt cggtcccgca
     4201 acggggcggc gcgttcgccg aacgcggcct gggcccgtgc gtactcggcc cgtccggacg
     4261 gggtctcgat gcggacgggg gagtagccga gatcggccag gtcgtacggg ctggcgcgca
     4321 tgtccagcac ccgcacctcg acagcgaggg cgaagcaatc cgccagcagc tccgacggcg
     4381 tggcaggggc gagcttgtac gcccatttgt agaggtccat cgtcgcgtgc aggcagcccg
     4441 gttgctcgtt cgcgatctgg tcggcgcgct cgggactgat cgcgttgagg ggacgggccg
     4501 cggccgtgaa gaatcggaac gcgtcgtgat gcgtgcagtg cacggatagc gactcgacca
     4561 cggcgtcggt tcctgctgct ccgagccgga gtgggacggc tccgtgccgg ggtgcatcgc
     4621 tgccgtacac catcgcccac tcgtgcagtc cgaagcagct cagccgggcc ggacgggacg
     4681 ccgtggcgag cagcaggtcc cgcacgaagg cggcggtgcc ggtgattcgg ggcggaaggg
     4741 cgtcgacgtc gaggcgtacg cagtcgccgt cccggacgtg ccccgggacc gccagcaact
     4801 cgtcgccgcc cctgagtagc gcacgcggcc ccgggtgcca cagctcgagt cgcgccggcc
     4861 ggagcgagta gtaggtgaac aggaagtcca tgacgggatg cgactcgccc cgacgccggc
     4921 gctcgcggtg gagcccggtc caccgcccga cccgggcccg gtgccgggct gcgcgggccc
     4981 gccacaccgg ctcctcgagc accacgggcg ctgcggaggt gagctcggtc gtcatggcag
     5041 ctcacggtac ggctgtggcg tgccggtcag gcaggccgca cccgccgggc gccgggggag
     5101 tcggggtcgt ccaggccgaa gaccaggatg cgctcagggt gtatgcggat cacacccggc
     5161 cgcactccgg ggaatcccgc aggcatggcg tccggcagag cctcagcggt tccgcggatc
     5221 tccagggccc gcggccgcca cggttccggc acgacgtcgt cgacgacgag cgcgacgcgg
     5281 ccgtcacgga ccacgttgcg ccatttctgc gtggacgggt tctgcaggcc cgggatgtcg
     5341 accgtgccgt tctcggcgtc gacgcggaag ccgacgggcc gtacctgcgg cgcgccgtcg
     5401 ggtccaatcg tggcgagccg ggcgaggcgc tgcccgcgga ggtaggccag ctcggccggg
     5461 gtgaagatcg aggtcatggt ggtcatggca caaccctgcg acctggagcc gggttcaggt
     5521 caagcctgcg atcgcggcgc gtgcgtggca tcgcggacct cgccgacgta gtgctccacc
     5581 aggtcctcca tcgccaccac cccgacggtc tcaccgcggc cgttcaccac ccgccccagg
     5641 tgggcgcgtg agcgccgcag caccgctagc gcctcgtcga ggcggaagga gacgggcacc
     5701 tcgggcaacc cgcggacacg ctcggggggc acggcggtat ccgggtcgtc gacaagatcc
     5761 aggatgtcct tcacgtgcag gtagccgatg agcttgccgt cgggggcagg taccgggaac
     5821 cgggagaagc cggtgtcggc gacggtttgg gccacgttct cgacggtcgg gttcgctggc
     5881 agcgtcacga gctcgtcgct cggcacgagg acgtcggcga cggtggcttc ggccgagctg
     5941 agcgtgcggg tgaggcgtcg cgactcgtgg tcgtcgagca ggccctcccg gcccgactcg
     6001 gcgatgaggt cggccagctc ccccgatgtg aaggaggtct cgagctcgtc gcgcgactcc
     6061 acgccgacga gccgcaggac gccgttggcc atcacgttga acagctcgat cagcgggcgc
     6121 atcacggtcg tgaagacgag gaacggcggc accagccaga tcgcggtccg ctcgggcccg
     6181 gcgatggcga tgttcttggg caccatctcg ccgagcagga tgtgcgcgac caccacgatg
     6241 gccagcgata ccgtgaacga gatcgggtgc agcagggctt cgggcagacc tgcccactgc
     6301 agcgggcgct cgatgaggtg cgcgaccgcg ggttcgccga gacggccgag cagcaacgag
     6361 gagatcgtga tacccagctg tgacgcggcc agcatgcggg acaggtccgc gccggcccgc
     6421 agcacggtgc gggcggctgg ggtaccggag accgccatcg cctcgagccg atcgcgccgc
     6481 gcggagatga gcgcgaactc ggctccgacg aagaaggcgt tggcgccgag cagcgccacc
     6541 gccgccagga gcaacagcag gtcgttaccc acgcgtggcc tcctcccgct tggcggtgga
     6601 tgcggcgcca ccggtgtcgg cacaggccac gcgcagttcg gcgatgcggt tgcggtcgcg
     6661 gcgcatgacc gtgagtcgcc acccgtcgac gacgacgtcc tcgccgacgt cggggatacg
     6721 tcccagccgg gcgagcacca tcccggccag cgtctcgtag tcgccggggg gcatctcgaa
     6781 ccccgtggcg tcggccacct catcgccgcg cagcagcccg gacaccagcc agctgccgcg
     6841 ccccagcgga cgcacctgcg cctgctcggc gcggtcgtgc tcgtcgcgca cgtcgccgac
     6901 gatctcctcg atgaggtcct cgagggtgac gatgcccgcc gtgccgccgt actcgtcgac
     6961 caccaccgcg agctggaggc cggagccgcg gagccgggtg agcagctcgt cgccgtcgag
     7021 cgaccccggc acggtcggga ccggttgaac catgtcgcgc agcacggcgc gctcccgctt
     7081 cgccgcgggc accccgaacg cctgcttgac gtgcaccacg ccgagcacgg cgtccggatc
     7141 cctgtcgtgc acagggaacc gcgagaaccc gctccgtcgc gcgagcatga ccaggtcggc
     7201 gacggtgtcg tcggcgtcga gggactcgac ccgcacccgt ggggtcatca ggtcctcggc
     7261 gacccggtcg gtgaaccgca gcgagcggtc gagcagggtg gcggtaccgg cgtcgagggt
     7321 gccgtgctcg gcgctggtgc gcaccagtga gctgagctcc tggggcgaac gggccgaccg
     7381 cagctcctcg gcgggctcga ccccgagccg ccgcacgacc gcgttggcag cactgttcaa
     7441 cccggtgatc aaccagcgga acgcccgggc gaagccgctc tgcaaccaga ccacggcccg
     7501 ggcggtgcgc aacgggtcgg cgatggcgag gttcttcggt acgagctcgc cgaacaccat
     7561 ggacagcgtc gtggccagga gcaacgacag cacggtggcg atgccggcgg ccagcccgtc
     7621 cgacaggccg atcgcggtga gaccgggccg gaagagggac gcgatcgcgg gctcggcgat
     7681 gtagccggtg accagcgtgg tcaccgtgat gccgagctgg ctccccgaca gctggaacga
     7741 cagcccgcgg tgggcgcgct gcacggcacg agcgcgccgg tcgccgacct cggcgacgtg
     7801 tgcgtcgacc tgactgcgct cgagcgcggt gagcgcgaac tccgatgcca ccgatatcgc
     7861 ggtgccgagg gtgagcgcgg cgacggcgat cagtccgagg acggacatca ggacgctcat
     7921 atcactccct cgctgcgctc ggtcggccca tcgtgagacg tcacagcgcg cgggcgcagt
     7981 gacactgatt cgcgcgcaag ctcgctcctc ggcggtcacg acagcgggtg tcgccacggg
     8041 aaggatggac agggcgccgg gtcgaacccg gcctactact gcaacccggg tcggcggggt
     8101 gtgcaggggg cacgggcgtg ctcctcgtca ggcggaacaa ccagtctaag gcgcgtggcc
     8161 cgcgcgggcc gaccttgccc accggacgag cggccgttac cggcctccgg gcaggcaccg
     8221 cattcccgac gtcacgccgg tcacgtctcg acgcggtggc ccgcgcgggg atctaagttg
     8281 acccgtcatg gacggcagtc gcaatggtgc gcgcgaggcg tcttcgccat tcgtggactt
     8341 cgacagggac gcctgggcga agctcggtga gaacacgccg ctgccactca cggccgagga
     8401 gctcgaccag gtacgcagcc tcggtgacga ggtcgacctc gacgaggtgc gggaggtcta
     8461 cctgccgctg tcgcgcctgc tcacgatgca cgtacaggag ggcgggcggc tctaccgcgc
     8521 gtaccggacg ttcctcggcg cgaaaactgc gcgcaccccc ttcgtcatcg gaatcgcggg
     8581 ttcggtgtcg gtcgggaagt ccaccacggc ccggttgatg cgcctgctgc tcgcccgctg
     8641 gccccagcac ccgcgcgtgg agctggtgac gaccgacggg ttcctgcacg ccaatgccga
     8701 gctggagcgg cgcgggctga tgacccgtaa gggtttcccg gagtcctacg accggcgggc
     8761 gctgctgcgg ttcgtcaccg aggtgaaggc ggggcgcgcc gaggtcacgg cccccgtgta
     8821 ttcgcacctg gtctacgaca tcgtccccga cgagcggctc accgtgcacc gtccggacat
     8881 cctgctgctc gagggactga acgtgctgca gcccgcacag ccggcgcggg agggcggcgc
     8941 ggcgctggcg gtgagcgatt tcatcgactt ctcggtctat gtcgacgccg ccaccgacga
     9001 catccggcgt tggtacgtcg aacgcttcct gcggttgcgg gagaccgcgt tccgggatcc
     9061 ggagtcctac ttccggcgct acgccgcact cgacgaggcc ggcgcgatcg cccgggccga
     9121 gagcatctgg gccgagatca acgggccgaa cctggagcgc aacatcaagc cgacgagggg
     9181 ccgggcgagc ctcgtgttgc gcaagggccc gaagcacaac atcacgggta ttcggttgcg
     9241 aaaggtgtga ccggaaagac gtctcgcgag cagcctggca cgatgtgcgg atgaccagcg
     9301 cacagcgcgt cgacaccgac gagcagaccg acctcgcagc gaggctgcgc ctggtcgtcg
     9361 gtaggttgca tcgccggatc cgcatcgacg gtcgggagtc gatcccgccg ctacagctgt
     9421 ccgctctcgc caccgtggag cagcacgggc cgttgcggct ctcggagctc gcccgccggg
     9481 aggcggtgac ggcaccgacg atgagcaggg tcctcgcggc cctggacgat cagggcctcg
     9541 tcatccgcac gcccgacccg agcgacgccc gcggggtgcg gatcgtgctg tccgaggaag
     9601 gcgccgcccg gctggccgag gtccgctcgc accgcacagc gctggtggcg cggcgactcg
     9661 cccgtctcga cgccgaccag cgccagatcc tcgaggcggc catgcctgcg ctcgaggcgc
     9721 tgctcgtcga cgacgactga ctcccagccg tggggcgtgt cagacctgct cgccgtcgac
     9781 cagcacccgc aggccgtcgt gcaggaagct cacccggccc ccgatgcgcg ccgactccgg
     9841 cagcggctcc gggtatcccc acacggcgtc ggtgagctcc cggccgtcgg ggagccgcgc
     9901 cgtccagtag ctcgcgtcgc ccttgtatgg gcagtgggtg ctggtggcgg tgggttcgag
     9961 tggcacctgc acgtcgtcgg gggagaggta ccagcgcagc ggcagcccgg tctcggcgag
    10021 catcagcggg gcattcgact cggctacgac ggtgtcaccg gcgagtacct gcacgtgccg
    10081 gctcgaccga cggatgtcga cgcggtggta tgggtcggtc agggcctgga cctcttcgtc
    10141 ctcgtcgaac caggcgtcgg cggcctccca gtacagcgac gcgtagccgg cgagccacgg
    10201 ggcctcggcc gtcgggtccg ggtacgccca cagcgcgttc tccacgacgc ggtcaccgac
    10261 ctgcacggac cggtaggtgg cgtcgccctt gaacgggcag tgcgtcctgt ggtcgctggg
    10321 gacgaacgcc gagggatcga tgtcggtctc gggaacgtag agccggggcg gcagcgcggt
    10381 ctcgtgcagc aggacgccct tgcgcgtgtc gagcaccgtc cggccggcga actcggcccg
    10441 cacgcggcgc ggaaacggat gcatgagcag cttgtgtgcg ggtccgtcga tgcggtagtt
    10501 gacggtcttc ggtgcgtgcg ccgacagtgg cgcgtcgctg cgagtcagtc ccatgtgggc
    10561 gcaacaccgc ccgctccctc gaacttcccg agggagcggg cgggccgtca caggtcgagc
    10621 gtcacgccgt agtcggtgaa ccaggcgtcg agggtcagta tctgctcgag ccgaacccgg
    10681 gcgcccatcc cggactggtc gttgacgggc tcggcaagcc gctcgcgcac ggcgacgggg
    10741 tcgagcagtt gcgccacggg tgagtcgggc cgttcgagca gcgccgcgag ctgcgcgcgc
    10801 agcatccgct cgtagccggc gtcctgcgtc gacgggtacg ggctcttcac ccgctgcact
    10861 accgagtcgg gcagaaggtc cgcggtggcg gcgcgcagca ggctcttctc ccgcccgtcg
    10921 aaggtcttgt gcgaccacgg cgtgccgaag acgtactcga cgaggcggtg gtcgcagaac
    10981 ggcacccgca cctcgagtcc gaccgccatg ctcatgcggt ccttgcggtc cagcagcacg
    11041 gggaggaagc gggtgaggta gagatagcag atctcgcgca tccggcgttc gtgcggatcg
    11101 gcggcggcag ggccggtcag cccgggcacc tcggcgagcg ccgtccggta ctgggcctcg
    11161 acgtagccgg ggagatcgag ctgcttggcc agggcctggt cgagcagccc cggtcgggtc
    11221 atgtcggtgt tcaacatccg cccggccatc caggggaacg tgtcggctgc gacggtgtcg
    11281 gcgtcatgga agtcgcggta accgccgaag acctcgtcgg ccgactctcc ggacagcgcc
    11341 acggtcgagt gttgccggac cgcccggaac agcaggtaga gcgacgtgtc gaggtcgccc
    11401 gtggtgatcg gtaggtcgcg cgcgtgcagt gtggcggccc gcgcgccggg ttccatcagc
    11461 tcatcggtcg acagcgtgat gtcggtgtgg acggtgccga cgaactcggc cacctcgtgg
    11521 acgtacggcc cgtccggggt gccgcgcacg tcgtcggcgg agaagttctc cgtgtagccg
    11581 gtgaagtcga cggcgaagga gcgcacgcga tccgtggtgt gtcgggcggc cagcgcggtc
    11641 agggccgacg agtcgagccc acccgagaga agcgtgcaca gtggcacgtc ggccaccagc
    11701 tggcgctgcg ccgtgtcctc cagcagctct cggacgtgcc ggaccgtggt gggcacgtcg
    11761 tcggtgtgcc cggtgtcctc gaggcgccag tagggcagct cgcggatgcc ctcgcgggtc
    11821 acccgcacca cgtggccggg tggcacctcc cgcagcccgc ggaacacgga gttcccgggg
    11881 ttggcgctga acgtgagcgc cctacgcagc ccgtcggcgt cgaccacgcg ggcggcgagg
    11941 gcgttggcca gcacggcctt gggctcggag ccgaacagca cgccatgcgg ggtggggtag
    12001 tagtagagcg gcttgacccc cagccgatcg cgcacgagca ccagctcgcg ggcaagcggg
    12061 tcccagagcg cgtaggcgaa catgccgttc agctcacgca ccgcgtcgcg cgggtcgcgc
    12121 cgaccccact cccggtgcgc gtgcagcac
//
LOCUS       DASRSC010000215        11632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4337565,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000215 DASRSC010000000
VERSION     DASRSC010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11632
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4337565"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..824
                     /locus_tag="VFE39_10540"
     CDS             45..824
                     /locus_tag="VFE39_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET6259077.1"
                     /translation="MSNVGGRDGGVQSVDRAISVLEILARRGEAGVTELAAEIDVHKS
                     TAFRLLGALEGRGLVEQAADRGKYRLGFGLIPLAGAVSDRLDVTRQGRPVCEQLAADL
                     GETINVAVLQEHWAVNVDQALGQSTVATHNWIGRLTPLHCTSSGKVLLAHLDAERRAA
                     LLAASGMPGFTPSTLTSPVALDPELERVRETGLALAVEEYEIGLNAAAAPVHDRSGEV
                     IAAVSVSGPAYRLGTDRLDELATPLRAGAAEMSRRMGWFRP"
     gene            1093..2145
                     /locus_tag="VFE39_10545"
     CDS             1093..2145
                     /locus_tag="VFE39_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HET6259078.1"
                     /translation="MITRSTLGLTVVLAAIAGCAVGGPATTASADAPAGNKDHACSPS
                     VSIDSFSDALDKTTFDNTFVGNLSALAVDTDGQIDALSDRSSLFTLDARTYKPTGVVS
                     LADENGQPLDSEGLALDRDGTRLITSEVEPSVRRYDRDGKLLGRLPVPDALRVAPAGR
                     ATTNQSFEGLTLQPGDRTLIASMEGPLAGDDADLVRFQTWNRHDAEADFALAAQYGYR
                     VDAGVGVSEITATGDGRLLVLERGFTAGVGNTVRLYLADPRDATDVSGVANLTAQTDA
                     RLIGKTLLADIGTCPSLGATAKQPQPNPLLDNIEAVAVTEKDNNRLRLLLSSDDNQNA
                     VQITRLYSLTAELPEQ"
     gene            complement(2155..3246)
                     /locus_tag="VFE39_10550"
     CDS             complement(2155..3246)
                     /locus_tag="VFE39_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HET6259079.1"
                     /translation="MIRTLPGSAYTDVPVFAAEQERIFERSWTCVALAADLAAPGAFR
                     TVTVGRESVVLVRGRDGAVRAFLNVCRHRGARLCTEESGQLRRNLQCPYHAWTYDLAG
                     KLVAAPNLTSMPDVRRDEYGLLAVHLREWLGYVWICLAPEPPSFEADVLGEVVTRLGD
                     ITAIESYGIEHLALGRRITYDVAANWKLIVENFMECYHCATIHPELTEVLPEFADGYA
                     AQYYVGHGAEFGSEIAGFTVDGTAGPVRLPGISADQDRRYYAITVKPQVFVNLVPDHV
                     IVHRMTPLAPDRTVVVCDWLFARDVVAAGSDIGPSVELFHRVNAQDFAACERTQPAMN
                     SRAYRDGGVLVPSEHHIGQFHTWVADRLA"
     gene            complement(3336..4466)
                     /gene="solA"
                     /locus_tag="VFE39_10555"
     CDS             complement(3336..4466)
                     /gene="solA"
                     /locus_tag="VFE39_10555"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HET6259080.1"
                     /translation="MNTYDVIVVGLGGMGSAAVRHLAARGVRVLGLERFTPAHDQGSS
                     HGGSRIIRQSYFEDPAYVPLLLRAYDLWAEAEKEADAELLTLTGGLYLGPPDSTTFAG
                     SLRAAQEWNLPHEVLDAAAVRARFPTISPAADEVAVFEERAGFVRPEAAVSAHLELAT
                     RNGADLRFAQQVQTWNAGTDGVRVRTADGEYSARHLVISPGAWAPSLLADLGLPLRVE
                     RQVQYWFAPPGGVAPFVGNPVYVAEQAGGDQIYGFPAIDGPDGGVKVAFFRHGRDTDP
                     DALDRVVTDAEVDEMRARVADTLPGLVGPVVKTVPCMYTTTPDEHFVIAPHPEHDNVT
                     VACGFSGHGFKFVPVVGEILADLATTGTTAHPIALFHPRRFT"
     gene            complement(4463..6883)
                     /locus_tag="VFE39_10560"
     CDS             complement(4463..6883)
                     /locus_tag="VFE39_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004560542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6259081.1"
                     /translation="MTPRVVVIGAGVVGAAVADELTARGWTDVTVLDRGTLPLPGGSS
                     SHAPGLVFQTNPSKTMAAFARYTVEKLSTFDGLLEESWCFNPVGGLEVATTPERLADL
                     RRRHGLATSWGIESRLVDPDECAALHPLVAADRVLGGFHTPTDGLAKAPRAVVAQLDR
                     ATDRGARVLGNHRVLQVLSDKGRVTGVRTDHGDVPADLVVSATGFWGPQTGALVDLTI
                     PLLPMAHQYVTTTPLAELAGRNTETAEASAPILRHQDRDLYFREHVDRVGIGSYAHRP
                     MPVDLDALPGTGMPSVLDFTPEDFAPSWADARELLPALRGAAIDSGFNGIFSFTPDGM
                     PLIGEHPALAGFWVAEAVWVTHSCGVGQALARWLVDGEPGIDVHECDLNRFEEAQLAP
                     SYVETRSAQNFVEVYDILHPLEPPAQPRPLRVSPFHARQVELGAVFLEAAGWERPHWF
                     EANAGLPEVSRIPGRGEWASRFWSPIAGAEALVTRDRVAMYDMTPLKRLEVTGPGALA
                     LLQRLTTNNLDKKPGTVTYTLLLEPSGGVRSDLTVARLGEQRFQVGANGNLDLDRLTR
                     EAGDDVAVRDITGATCCIGLWGPRARDVLASVTRDDVSHAGFGYFRARPIHVGGVPVT
                     AMRLSYVGELGWELYTSAELGLRLWDVLWDAGRRYGVIAAGRSAFNSLRLEKGYRGAG
                     TDMTTEHDPYEAGLGFAVRPGSYVGSSAIAGRQSPARRLVPLLLDEPAHVVMGKEPVW
                     HAGLPVGYVTSAAYGYTIDASIAYAWLPAEAAEPGTAVHVEYFGERLPAVVAAEPLFD
                     PEMKRIRS"
     gene            complement(6880..7209)
                     /locus_tag="VFE39_10565"
     CDS             complement(6880..7209)
                     /locus_tag="VFE39_10565"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HET6259082.1"
                     /translation="MIHVCALAELPRGTARRLPLSPPVSVFHTEDGELYAIDDTCTHQ
                     DASLADGWLEGCEVECPLHAAIFDLRTGEPDGLLAREPVRTHRVVVEADTVYVVLAGH
                     DALEAAS"
     gene            complement(7353..8132)
                     /locus_tag="VFE39_10570"
     CDS             complement(7353..8132)
                     /locus_tag="VFE39_10570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259083.1"
                     /translation="MPRLHAAFSLGTVAGAVLGAASAAAGVPIEVQIIMVAALIPVVA
                     TVSTRRFLPVQESTPEQKAAGSGALTAWREPRTLLVGLLVLGFAFTEGSANDWIAVAM
                     VDGYGGGETIGAVAFGFFVTAMTLGRLVGGAALERFGRVAVLRATAAIALAGLLLVVL
                     GGSTPVALGGALLWGVGASLGFPVGMSAAADDPARAAARVSVVSSIGYTAFLAGPPLI
                     GLLAEHVGILRALFVVLAALVLGLLASGATRPLPVTEPAAR"
     gene            complement(8136..8555)
                     /locus_tag="VFE39_10575"
     CDS             complement(8136..8555)
                     /locus_tag="VFE39_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259084.1"
                     /translation="MTTTGISPAADILRARIAVVVAFAANGLAFASFISRTPAIRDTL
                     GLSSGQLGLLLLCLSGGAVTGLPLSGPLVHRLGPGRAVLAGALSESIGLALLVVGLST
                     GVVAPAAIGLVLTGLGTGVWDVAMNVEGADVERRLGR"
     gene            8653..9705
                     /locus_tag="VFE39_10580"
     CDS             8653..9705
                     /locus_tag="VFE39_10580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6259085.1"
                     /translation="MSTRPTLAAVAACAGVSPSTASLVFSGSPRVTPSTRERVLAAAA
                     TLGYAGPDPLAASLRRGRSGVIGAFVGERLLYAFRDPVAVQLLDGITEVLGPHGVGLL
                     LLAGDTGRPSTEQIGRIPLDAAIFATCGLEDDPALDQLRARGVPLVVVEGPVLDGVVL
                     IDIDDRRGTRELARHLHDLGHRRVEVLTMPLRLDGRRGPIDGARRARAHYRDVRHRLE
                     GVEDVFGPVPAVEAASNAVDEGTAAGRALLDVPAHRRPTAVIAHSDMLAAGVLQAAAD
                     LGLRVPEDVSVAGFDGADLHWLGDTRLTTVVQPTDEKGRAAAQAAMDLVAGGSPRDLT
                     LPVTLRIGTTSGPAPS"
     gene            complement(9861..10757)
                     /locus_tag="VFE39_10585"
     CDS             complement(9861..10757)
                     /locus_tag="VFE39_10585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HET6259086.1"
                     /translation="MQRYEWLRRIERLDPETDFYEIHRISSAHEFPWDYIQSLSFALF
                     RTYAVPSIGELLASTGEFTERVQKRYDDTGLILDAVVEHGFASQQGRAAIRRMNQMHG
                     AYDISNADMLYVLSTFVTVPIRWIDELGWRRLSEQEKIAGANYYRELGRHMGIKDIPV
                     THQEFAAHLDDYEAEHFAFHPGARAVADSTLDLFTTFPPNHRAPAGLVKRFSYGLMDD
                     PLRAAFRYPRISAFEQALARRAVWLRGRVVRFMRPREEPHFARQLPNFRSYPDGHDVS
                     TLGTFPRGCPVPHEAPAGESAA"
     gene            10825..11460
                     /locus_tag="VFE39_10590"
     CDS             10825..11460
                     /locus_tag="VFE39_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6259087.1"
                     /translation="MTSPVDAHSPAAPRGRREERTATSRARILDAAVACLVESGYAGA
                     TTLRIQAQAGVSRGRLLHHFPSRDQLLVAASRHLATERLRRTAERIESLAGGLSGVER
                     LDRAIELMWETFSEPHFWAAVELWTAARTNEDLRAALLPEEREIGAAIRAGMDRFYGP
                     EVIEHPHYPQVRELLFTSMRGVGLTYALEHRDPHTDPHLDLWKQTARTLVT"
BASE COUNT         1749 a         4241 c         4087 g         1555 t
ORIGIN      
        1 tcgccgtcaa gcagtgtgcc cgttttgatg catatagttt cgccatgagc aacgtcggcg
       61 ggcgagacgg tggagtgcag tccgtcgata gggcgatcag cgtgctggag atcctggccc
      121 gccgcgggga ggcgggggtc accgagctgg cggccgagat cgacgtccac aagtccacgg
      181 cgttccggct cctcggcgcc ctggagggtc gcggcctcgt cgagcaggcc gccgaccgcg
      241 gcaagtaccg gctcgggttc gggctgatcc cgctggccgg agcggtttcc gaccgcctcg
      301 acgtcacccg gcagggccgc cccgtctgcg agcaactggc ggccgacctc ggcgagacga
      361 tcaacgtggc cgtgctgcag gagcactggg ccgtgaacgt cgaccaggcg ctgggccagt
      421 ccaccgtcgc cacccacaac tggatcggcc ggctcacgcc gctgcactgc acatcgagcg
      481 ggaaggtact gctcgcccat ctcgacgccg aacgccgcgc cgcgctgctc gccgcgtccg
      541 ggatgccggg gttcacgccg agcacgctca cgtctccggt ggcactcgac cccgagctgg
      601 agcgggtgcg cgagacgggc ctcgcgctgg cggtcgagga gtacgagatc gggctcaatg
      661 ccgccgcggc acccgtgcac gaccgttccg gcgaggtgat cgcggcggtc agcgtgagcg
      721 gcccggccta ccgcctgggt acggaccggt tggacgagct cgcgacgcca ctacgagcgg
      781 gtgcggcgga gatgagcagg aggatgggct ggttcaggcc gtgaacggat cttcgcggcg
      841 atctcatcgg gttcgccgta cacctggttc ttgacgaagc ggtacatccg ccagccctgg
      901 tccaccagag tcgtgtagcg gcccacgtca cgcaacacgc gctcgcggct gccgtggtcg
      961 gcaccctcgt actccatgcc gatccggtgg gctcgtcgtg agatgaacac cagagctggt
     1021 ctcccggagt tccaacagcg ctcatctcca cttcccatcc gttgccccgc ggttctcccc
     1081 tagcgtggcg ccgtgatcac gcgatctact ctcggcctca ccgtcgtact ggcggcgatc
     1141 gccggttgtg ccgttggcgg accggccaca accgcgagcg ccgatgcccc ggccggcaac
     1201 aaggaccacg cctgctcgcc gtcggtgagc atcgactcct tctccgacgc gctggacaag
     1261 accaccttcg acaacacctt cgtcggcaac ctctcggccc tcgcggtcga cacggacggc
     1321 cagatcgacg ccctctccga ccggtcgtcg ctgttcaccc tggatgcgcg gacgtacaag
     1381 ccgaccggcg tcgtgagcct cgcggacgag aacggccagc cgctggactc cgaaggcctg
     1441 gccctcgacc gcgacggcac ccgcctgatc acctccgagg tcgagccgtc cgttcgccgg
     1501 tacgaccgtg acggcaagct cctcggccgg ctcccggttc ccgacgccct gcgcgtggcc
     1561 ccggccggac gcgccaccac gaaccagtcc ttcgaggggc tgaccctgca gcccggggac
     1621 cgcacgctga tcgcctcgat ggagggtccg ctcgccggcg atgacgccga cctggttcgc
     1681 ttccagacct ggaaccgcca tgacgcggag gccgacttcg cactcgccgc ccagtacggc
     1741 taccgcgtgg acgccggggt cggcgtctcc gagatcaccg ccaccggtga cggccgcctc
     1801 ctggtgctgg aacgcggctt caccgccggc gtcggcaaca ccgtccgcct gtacctcgcg
     1861 gacccgcggg acgcgaccga cgtcagcggc gtagcgaatc tgaccgcgca gaccgacgcc
     1921 cggctgatcg gcaagacgct gctggcggac atcggcacgt gccccagcct cggtgccacc
     1981 gccaagcagc cccagcccaa cccgctgctc gacaacatcg aggccgttgc cgtcacagag
     2041 aaggacaaca accgcctgcg gctgttgctg agcagcgacg acaaccagaa cgccgtccag
     2101 atcacgcggt tgtactcgct cacggcggag ctgcccgagc agtaggtccg ctgctcacgc
     2161 caggcggtcg gccacccagg tgtggaactg tccgatgtgg tgctcgctgg gcaccagcac
     2221 gcccccgtcc cggtacgccc ggctgttcat cgccggttgg gtgcgctcgc aggccgcgaa
     2281 gtcctgggcg ttgacgcggt ggaacaactc caccgacggg ccgatgtcac tccccgccgc
     2341 caccacgtct cgagcgaaca gccagtcgca caccacgacc gtgcggtccg gggcaagggg
     2401 ggtcatccgg tgcacgatca cgtggtcggg caccaggttc acgaagacct gcggcttgac
     2461 cgtgatcgcg tagtagcggc ggtcctggtc ggcggagatg ccggggagcc gcacgggacc
     2521 ggcagtcccg tcgacggtga acccggcgat ctccgatccg aactccgcgc cgtgccccac
     2581 gtagtactgc gccgcgtacc cgtcggcgaa ctcgggcagc acctccgtca gctccgggtg
     2641 gatcgtcgcg cagtggtagc actccatgaa gttctcgacg atcagcttcc agttcgccgc
     2701 cacgtcgtac gtgatccgcc ggcccaacgc caggtgctcg atgccatagc tctcgatcgc
     2761 cgtgatgtcc ccgagccgcg tgacgacctc gccgagcacg tccgcctcga aggaaggtgg
     2821 ttcgggagcg aggcagatcc agacgtatcc gagccattcc cgcaggtgga cggcgagcag
     2881 cccgtactcg tcgcggcgga cgtcgggcat cgacgtcaga ttgggagcgg cgaccagctt
     2941 cccggcgagg tcgtaggtcc acgcgtggta ggggcactgc aggttgcgcc gcagctgccc
     3001 ggactcctcc gtgcacagcc gcgcaccacg gtggcggcag acgttcagga acgcgcgcac
     3061 ggcgccgtca cggccgcgga cgagcaccac gctctcccgg ccaaccgtca ccgtgcggaa
     3121 cgcaccgggc gcggcgaggt cggcggcgag tgcgacacac gtccagctgc gctcgaagat
     3181 cctctcctgc tccgcggcga acaccggcac gtccgtgtag gccgagccgg ggagggtgcg
     3241 gatcagtgac tgctggccga ggccgggctg cccgggcggg ggatgctcgt cgagcgcggg
     3301 ctgcccagga cctggttgcc cagaatgcac agcggtcacg tgaacctccg cgggtggaac
     3361 agggcgatgg ggtgggcggt ggtgccggtg gtggccaggt ccgcgaggat ctccccgacc
     3421 accggcacga acttgaaccc gtggccggag aacccgcagg ccacggtgac gttgtcgtgc
     3481 tcggggtgag gagcgatcac gaagtgctcg tcgggggtgg tggtgtacat gcacggcacc
     3541 gtcttcacga ccggccccac cagcccgggc agggtgtcgg cgacgcgggc ccgcatctca
     3601 tcgacctcag cgtcggtgac cacgcggtcg agcgcgtcgg gatcggtgtc ccggccgtgg
     3661 cggaagaacg cgaccttgac cccgccgtcc ggtccgtcga tggcggggaa tccatagatc
     3721 tggtcacccc cggcctgctc agccacgtag acggggttgc cgacgaacgg agcgacgccg
     3781 ccgggcggcg cgaaccagta ctggacctgc cgttcgacgc gcagcgggag cccgaggtcg
     3841 gcgagcaggc tcggcgccca cgcgcccggt gagatcacca ggtggcgggc gctgtactcg
     3901 ccgtcggcgg tacgcacgcg cacgccatcg gtcccggcgt tccacgtctg cacctgctga
     3961 gcgaaccgca ggtcggcccc gttacgggtg gccagctcca ggtgcgcgct cacagccgcc
     4021 tcgggacgga cgaaccccgc gcgctcctcg aacaccgcca cctcgtcggc ggccgggctg
     4081 atcgtcggga agcgggcccg gaccgcggcg gcgtccagca cctcgtgcgg gaggttccac
     4141 tcctgcgcgg cgcgcaggct gcctgcgaac gtcgtgctgt cgggcggacc gaggtagagg
     4201 ccaccggtga gggtgagcag ctcggcgtcg gcctccttct ccgcctccgc ccacaggtcg
     4261 taggcccgca gcagcagtgg gacgtaggcg gggtcctcga agtaggactg ccggatgatc
     4321 cgcgagcccc cgtggctgga accctggtcg tgcgccgggg tgaaccgctc gagcccgagc
     4381 acccgcaccc cccgcgccgc gaggtgccgg accgccgccg accccatccc cccgagcccg
     4441 accacgatca cgtcataggt gttcacgatc tgatccgctt catctcgggg tcgaagaggg
     4501 gttcggcggc caccaccgcc gggagacgct cgccgaagta ctccacgtgg accgccgttc
     4561 caggttccgc ggcttccgcc ggaagccagg cataggcgat gctcgcgtcg atcgtgtagc
     4621 cgtaggccgc gctggtgaca tacccgacgg ggagcccggc gtgccacacc ggctccttgc
     4681 ccatgacgac gtgtgcaggc tcgtcgagca gcagcgggac cagccgtcgg gcgggggact
     4741 gccgcccggc gatcgccgag ctgccgacgt aggaccccgg ccgcaccgcg aaccccagcc
     4801 cggcctcgta cgggtcgtgc tccgtggtca tgtcggtgcc ggcaccgcgg tagcccttct
     4861 ccaaccgcag cgagttgaag gcgctgcgcc ccgccgcgat cacgccgtag cgccggccgg
     4921 cgtcccacag cacgtcccac agacgcagcc ccagctcggc gctggtgtag agctcccagc
     4981 ccagctcacc gacgtaggac aaccgcatgg ccgtgacggg cacgccgccg acgtggatcg
     5041 ggcgggcccg gaagtagccg aacccggcat gcgacacgtc gtcgcgcgtg accgacgcca
     5101 gcacgtccct ggcgcgtggc ccccagagcc cgatgcagca cgtggcgccc gtgatgtcgc
     5161 ggaccgccac gtcgtcgccc gcctcgcggg tgagccggtc caggtccagg ttgccgttgg
     5221 cgcccacctg gaaccgctgc tcgcccagcc gcgcgacggt cagatcgctg cgcacgcctc
     5281 cggagggctc cagcagcagg gtgtaggtga ccgtgcccgg cttcttgtcc aggttgttgg
     5341 tggtgagccg ctgcagcagc gccaacgcgc cgggaccggt gacctccagg cgcttgagcg
     5401 gggtcatgtc gtacatcgcc acccggtcac gggtgaccag cgcctccgcg cccgcgatcg
     5461 gcgaccagaa ccgcgacgcc cactccccgc gccccgggat gcgtgacacc tccggcaggc
     5521 cggcgttggc ctcgaaccag tgcggccgct cccagcccgc cgcctccagg aacacggccc
     5581 cgagctccac ctgccgggcg tggaacgggc tcacccgcaa cggccgcggc tgcgcgggcg
     5641 gctccagcgg atgcaggatg tcgtagacct ccacgaagtt ctgcgcgctg cgggtctcca
     5701 cgtaggacgg cgcgagctgc gcctcctcga accggttgag gtcgcactcg tgcacgtcga
     5761 tgccgggctc cccgtccacc agccagcgcg caagcgcctg tccgacaccg caggagtggg
     5821 tcacccacac cgcctcggcc acccagaagc ccgccagggc cgggtgctcg ccgatgagcg
     5881 gcatgccatc gggggtgaag gagaagatgc cgttgaagcc gctgtcgatc gccgcgccgc
     5941 gcagggcggg cagcagctca cgtgcatccg cccaggacgg tgcgaaatcc tcgggggtga
     6001 agtcgaggac gctcggcatg cccgtcccgg gtagcgcgtc gaggtcgacg ggcatggggc
     6061 ggtgcgcgta ggagccgatg ccgacgcggt cgacgtgctc ccggaagtac aggtcgcggt
     6121 cctggtggcg caggatcggc gcgctcgcct cagcggtctc ggtattgcgc ccggccagct
     6181 cggcgagcgg cgtcgtcgtc acgtactggt gtgccatcgg cagcaacggg atcgtcaggt
     6241 cgaccagcgc acccgtctgc ggcccccaga acccggtggc ggagacgacg agatcggcgg
     6301 ggacgtcgcc gtgatcggtg cggacgcccg tgacccgccc cttgtccgac agcacctgaa
     6361 ggacccggtg gttcccgagc acccgggcac cgcggtcggt ggcgcggtcg agctgcgcca
     6421 cgacggcccg gggggccttg gccagcccgt cggtgggggt gtggaagccg cccagcaccc
     6481 ggtcggcggc gaccaggggg tgcagggccg cgcactcgtc cgggtcgacg aggcgggact
     6541 cgatgcccca gctcgtggcc aggccgtgcc tgcgcctcag gtcggcgagc cgctcgggtg
     6601 tcgtcgcgac ttcaagcccg ccgaccgggt tgaagcacca tgactcctcc agcagcccgt
     6661 cgaacgtgga cagtttctcg acggtgtagc gggcgaaagc ggccatcgtc ttcgatgggt
     6721 tggtctgaaa caccagaccg ggagcgtgcg aggacgaccc gccgggcaac ggcagcgtgc
     6781 cccggtcgag cacggtgacg tcggtccagc cgcgggccgt gagctcgtcg gcgacagcgg
     6841 caccgacgac gccggccccg atgacgacga cccggggcgt catgatgcgg cttccagggc
     6901 gtcgtgccct gcgagcacga cgtagacggt gtcagcctcg acgaccacac ggtgggtgcg
     6961 gacgggctcg cgggcgagca ggccgtcggg ctcgccggtg cgcagatcga agatcgcggc
     7021 atgcaacgga cattcgacct cgcatccttc gagccagccg tcggcgagcg aggcgtcctg
     7081 gtgggtgcac gtgtcgtcga tggcatagag ctcgccgtcc tcggtgtgga acaccgagac
     7141 cggcgggctg agcgggaggc ggcgggcggt gccgcgggga agttctgcga gtgcgcagac
     7201 gtggatcatg ctggctctcc gaagcgcagt gcggaacgtg gtgcacgact cgcaacaggg
     7261 ttcgctgttg atcgaacgcc gtcaagagcc gacgcgccct ggaaaggcga ccgtaacctg
     7321 cgcaccctgg ccgaaagccg gggtttgccg gctcagcggg cggcgggttc cgtcacgggc
     7381 agcggacggg tggcccccga ggcgagcagc cccagcacga gggcagcgag cacgacgaac
     7441 aatgcgcgca ggatgccgac gtgctcggcg agcagtccga tcaacggcgg gccggccagg
     7501 aacgcggtgt agccgatcga gctgaccaca gagacacggg cggcggcgcg ggccgggtcg
     7561 tcggccgccg cgctcatgcc gaccgggaag cccagcgagg ccccgacccc ccagagcagg
     7621 gccccgccga gcgcgacggg cgtcgacccg ccgagcacca cgagcagcag cccggcgagc
     7681 gcgatcgccg cggtcgcgcg cagcacggcg acgcggccga accgttccag cgccgcgccc
     7741 ccgaccagcc gcccgagcgt catcgcagtg acgaagaacc cgaacgcgac cgcgccgatc
     7801 gtctcgccgc cgccgtagcc gtcgaccatc gcgacggcaa tccagtcgtt ggccgagccc
     7861 tcggtgaacg caaacccgag caccagcagc ccgaccagga gcgtccgcgg ctcgcgccac
     7921 gcggtgagag cgccggaacc ggccgccttc tgctccggtg tcgactcctg cacgggcagg
     7981 aaccgccggg tggaaacagt ggccacgacg gggatcaggg cggccaccat gatgatctgc
     8041 acctcgatcg gcacccccgc ggcggcagac gccgccccca gcacagcacc ggccacggtg
     8101 ccgaggctga acgccgcgtg cagccggggc atgagctagc ggccgagcct gcgctcgacg
     8161 tcagccccct cgacgttcat cgcgacgtcc cagacgccgg tgcccagtcc ggtcaggacc
     8221 aggccgatcg ccgcaggcgc cacgacgccg gtggacaacc cgaccaccag cagggccagc
     8281 ccgatgctct ccgacagtgc gccggccagc acggcgcgac ccggcccgag ccggtgcacg
     8341 agcggcccgg agagcggcag cccggtgacg gcgccgccgg acaggcacag caggagcagg
     8401 ccgagctgtc ccgacgagag tccgagcgtg tcccggatcg caggcgtgcg ggagatgaaa
     8461 gaggcgaacg ccagcccgtt ggcggcgaac gcgacgacca cggcaatgcg ggcgcgaagg
     8521 atatcggcgg cgggtgatat cccggtcgtc gtcacagctg ctcctgtcga atcgattcga
     8581 tcgccgtcga ccgtacgcgc cgatccccgg cggcctcaac cggcaatcgc gtgatccggc
     8641 taccgttcca ccgtgtccac ccgcccaacc ctcgcggccg tggcggcgtg cgccggggta
     8701 tcgccctcga cggcgtcgct ggtgttctcc ggcagcccgc gggtcacgcc cagcacgaga
     8761 gaacgcgtgc tggcggccgc ggccacgctg ggctacgccg ggcccgaccc gctcgccgcc
     8821 tcgctgcgcc gtgggcgcag cggggtgatc ggcgcattcg tcggcgagcg gctgctctat
     8881 gcgttccgcg acccggttgc cgtccagctg ctcgacggga tcaccgaggt gctgggaccg
     8941 cacggcgtcg ggctgctgct gctcgcagga gacaccgggc gtccctcgac cgagcagatc
     9001 ggccgcatcc cgctcgatgc cgcgatcttc gcgacctgcg gcctcgagga cgatcccgcg
     9061 ctcgaccagc tgcgggcccg cggagtaccg ctcgtcgtcg tcgagggccc ggtgctggac
     9121 ggcgtcgtgc tcatcgacat cgatgaccgg cgcggcaccc gcgaactcgc ccggcacctg
     9181 cacgacctcg gtcaccgccg cgtcgaggtc ctcacgatgc cgctgcgcct cgatggccgc
     9241 cgcggcccca tcgacggcgc ccggcgggcc cgcgcccact atcgcgacgt gcgccaccgg
     9301 ctggagggcg tggaggacgt tttcggcccc gtgcccgccg tcgaggcggc cagcaacgcc
     9361 gtcgacgagg gcaccgcagc cggacgcgcg ttgctcgatg taccggccca ccgccgcccc
     9421 accgcggtga tcgcacacag cgacatgctc gccgccggag tgctgcaggc cgcagcggac
     9481 ctcgggctgc gcgtaccgga ggacgtcagc gttgcagggt tcgacggcgc cgacctgcac
     9541 tggctcggtg acacccggct caccaccgtt gtgcagccga ccgacgagaa ggggcgcgcc
     9601 gccgcccagg ccgcgatgga cctcgtcgcc ggcggcagcc cgcgtgacct gacgctgccg
     9661 gtgacactgc ggatcggcac cacgagcgga ccggcaccgt cctgagggag tcgtgcgcgg
     9721 cgaggtgggt caggcggcac cccgcgccag cctcatcccg tttcgtgcgc cggagcaggc
     9781 gtggatggcc ggccgctgac gcggctcgtg cgcggatacg agtgccggag cactcgtatc
     9841 cgcgcacagc ggagggcggg tcaggcggcg ctctcgccgg cgggtgcctc gtgcggcacg
     9901 gggcaaccgc gcgggaacgt gcccagcgtg ctcacgtcgt ggccgtcggg gtagctgcgg
     9961 aagttgggca gctgccgggc gaagtgcggc tcctcccgcg gccgcatgaa ccgcaccacc
    10021 cgcccgcgca gccacaccgc gcgccgcgcc agcgcctgct cgaacgcgga gatccgtggg
    10081 tagcggaagg cggcgcgcag cgggtcgtcc atcagcccgt aggagaaccg cttgaccaac
    10141 ccggccggcg cgcggtggtt gggcgggaac gtcgtgaaca ggtccagcgt gctgtcggcg
    10201 acggcacggg cgccggggtg gaacgcgaag tgctcggcct cgtagtcgtc gaggtgggca
    10261 gcgaactcct ggtgcgtgac cgggatgtcc ttgatgccca tgtgtcgccc gagctcgcgg
    10321 tagtagttgg cgccggcgat cttctcctgc tccgagaggc gtcgccagcc gagctcgtcg
    10381 atccaccgga tcggcaccgt gacgaacgtc gagagcacgt agagcatgtc ggcgttcgag
    10441 atgtcgtacg ccccgtgcat ctggttcatc cggcggatcg cagcgcggcc ctgctggctg
    10501 gcgaagccgt gctcgacgac ggcgtcaagg atgaggccgg tgtcgtcgta acgcttctgc
    10561 acccgctcgg tgaactcgcc cgtcgacgcg agcagctcgc cgatgctcgg cactgcgtag
    10621 gtgcggaaca gcgcgaagga cagtgactgg atgtagtccc atgggaactc gtgggcgctg
    10681 ctgatgcggt ggatctcgta gaagtcggtc tccgggtcga gccgctcgat ccgccgcaac
    10741 cattcgtacc gctgcatgcg cggagagtag gacggacaaa ccgaaagggt caacactgac
    10801 tgctaacgac tacccttccc ggacatgacg tcccccgtcg atgcgcactc ccccgccgcc
    10861 ccgcgcgggc gtcgcgagga gcgcacggcc accagccggg cccgcatcct cgacgcggcg
    10921 gtcgcgtgcc tcgtcgagtc cgggtacgcg ggcgcgacga ccctgcgcat ccaggcacag
    10981 gccggggtgt cccgcggtcg cctgctgcac catttcccct cgcgcgacca gctcctcgtc
    11041 gccgcgtccc ggcacctggc gaccgaacgc ctccggcgca ccgccgaacg gatcgagagc
    11101 ctcgccggcg gcctctcggg cgtcgagcgc ctggaccgcg ccatcgagct gatgtgggag
    11161 accttctccg agccgcattt ctgggccgcc gtcgagctgt ggacggcagc ccgcacgaac
    11221 gaggacctgc gcgccgcgct gctgcccgag gaacgcgaga tcggcgcagc catccgcgcc
    11281 ggcatggatc ggttctacgg cccggaggtg atcgagcacc cgcactaccc gcaggtccgc
    11341 gagctactgt tcaccagcat gcgcggcgtc gggctcacct acgcgttgga gcaccgcgac
    11401 ccgcacaccg acccccacct ggacctctgg aagcagaccg cccgcaccct cgtgacctga
    11461 cccgcgagtc gcccaatccc cgcccccgag tcgcccaatc cccgcccgcg agtcgcccga
    11521 ttcgcgcccg cgagtcgccc gcattccgcc cacgagtcgc ccacattcct cccgcgagtc
    11581 gcccgagccc tgagccaccg gtctcccgct agccgggctg cggagccacc gg
//
LOCUS       DASRSC010000216         6211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4504265,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000216 DASRSC010000000
VERSION     DASRSC010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4504265"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..1737)
                     /locus_tag="VFE39_10595"
     CDS             complement(28..1737)
                     /locus_tag="VFE39_10595"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET6259088.1"
                     /translation="MPAPPEEVNPAELPQGPPPPLPDGALRVVALGGIGEIGRNMTVF
                     EYDGRLLVVDCGVLFPAEDSPGVDLILPDFRAIEDRLDDIDALVLTHGHEDHIGAVPF
                     LLRQRPDLTVVGSRFTLALVAAKCKEHRLTPHLLEVKEGARLRHGSFDCEYFAVNHSI
                     PDALAVAIRTPAGLVLHTGDIKLDQLPLDGRLTDLAGFSRLGDEGVDLFLVDSTNADI
                     PGFVIAEREIGPVLDGIFHRAEARIIVACFASHVHRVQQVLDAAAEHGRKVCFVGRSM
                     VRNMGLAAELGFLNVPEGLLIELDEAMELPDDKLVIVSTGSQGEPLSALARMARGDHR
                     SVSIKPEDTVILASSLIPGNETAVYAVINGLTRLGASVVHQGTAKVHVSGHSPAGELL
                     FLYNAVRPSNVMPVHGEWRHLRANAVLATRTGVPEDSVVLAEDGVVVDLVDGRASITG
                     RVEVGRVYVDGLAVGDIGESTLGDRLVLGEGGFISITVAVDSTTGRAVSRPTVSGRGF
                     SDDPKALDAVVQLVEDELSRTEAEGITDTHRIAQSVRRVVGKWVGETYRRRPMIVPTV
                     IPV"
     gene            complement(1859..2857)
                     /gene="dapA"
                     /locus_tag="VFE39_10600"
     CDS             complement(1859..2857)
                     /gene="dapA"
                     /locus_tag="VFE39_10600"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET6259089.1"
                     /translation="MSPTPAGPRPSGRPFGRVLTAMVTPFDADGHLDLDKAEEVAAHL
                     IDLGNDGLVLNGTTGEGPTTTDREKSDLVRAVVSAVGNRATIVAGAGTYDTAHSIELS
                     RAAEKAGAHGLLIVTPYYSRPPQAGLVLHFTAIADATDLPVMLYDIPPRSVIPIEIET
                     LQRLAEHPRIVAVKDARNDLRVGTEVLATTPLAYYSGDDPVNLPWLSVGAVGFVSVIG
                     HVVANRLREMLDAYEAGEVDRARTLNASALPVIRAMGRVGGVVFAKAALGLRGLDVGC
                     PRLPLPPATEEQVAAIAGDLGAAGIALDPDAYRRTDRGHGALGASSAELGVEVAYR"
     gene            complement(3052..3678)
                     /locus_tag="VFE39_10605"
     CDS             complement(3052..3678)
                     /locus_tag="VFE39_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03085 family metal-binding protein"
                     /protein_id="HET6259090.1"
                     /translation="MTLASSERAAICDELERVGPDRPTLCEGWSTRHLLAHLLVRERQ
                     PWAAPGIVVPALAPFTERAMGGYEGAPWGDMVGQLRAGPPLWSPFRIPKLDEVGNGIE
                     FFVHHEDVRRGEPGWEPRPPDPVRDAKLWAAVVRMGSGLYRHSPVGIVLRRPSGDQHV
                     VRTGAGLVTIVGEPAELVLHAFGRDAARVELEGDPTAVEAVAAAARGV"
     gene            complement(3764..4804)
                     /locus_tag="VFE39_10610"
     CDS             complement(3764..4804)
                     /locus_tag="VFE39_10610"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6259091.1"
                     /translation="MRPARAGRTEHARGRWQRTSVRVALVARLARVAYLAPVALSAAL
                     PWAWFAVRDAIGPVTDVLAIVLPAVVALIATAAVVGAVRLRRRRWLAPAASVLVLGCA
                     TVVGPWLPSDAGRVAPGRAVTVVAANVMGSPESADELLALQADVLVVSEATDDLAPVL
                     VPAYPYRERAVGGPEVAVYSRLPLRLVESQAPDLPGLRLEVAGPAGPFVLYALHVPRP
                     WFTTVGAYQATVTEHHGIMRALAGRVAGEALPVVVTGDLNTPDRGRDYREILRDGGLV
                     DAMRDGWGGYTSTGQWAPLLLRIDHVLVSTGWCGDAPRRVALPGSDHHAVASSVGPCN
                     AARTEPSLGSAR"
     gene            complement(4801..5553)
                     /gene="thyX"
                     /locus_tag="VFE39_10615"
     CDS             complement(4801..5553)
                     /gene="thyX"
                     /locus_tag="VFE39_10615"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HET6259092.1"
                     /translation="MPQIVPLGVQLVAKTEFVAPEGVPWSTDADGGQALAEFAGRACY
                     QSWAKPNPSTATNAGYLRHILQVGHLSVLEHGSVSFYLTGVSRSLTHELIRHRHFSYS
                     QLSQRYVPERDAAMVEPDVIAADPELHNLFTEAAQASVTAYEALLEGLEKRFADVPNA
                     TLRRKQARQAARSVLPNATETRIVVTGNYRAWRHFIAMRATEHADVEIRELAIECLRQ
                     LQREVPNVFGDFEIATLEDGTEIASSPFVTEG"
     gene            5653..6171
                     /locus_tag="VFE39_10620"
     CDS             5653..6171
                     /locus_tag="VFE39_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HET6259093.1"
                     /translation="MDLSQYVTQLREDLASAAAAGDEQTRRTAALLGAAIEPAARLAL
                     MNALSDLAAEVTAALPDRVVEVRLDGRDVRVAVTADPAAEPAAEEPPPPPFRGFPPGA
                     DAGDISRITLRLMEQMKNQAEQAAAAQGVSLNSWLAQAVQGALAGGGRRGRRNWTQGD
                     RDERRLRGWVEG"
BASE COUNT          910 a         2244 c         2251 g          806 t
ORIGIN      
        1 accgcggcgc ggctgcaggt cgggcggtca gacggggatc accgtcggca cgatcatcgg
       61 ccgcctgcgg taggtctcgc cgacccactt gcccaccacc cggcgcaccg actgggcgat
      121 gcggtgggtg tcggtgatgc cctcggcctc tgtccgcgac agctcgtcct ccaccagctg
      181 caccacagcg tcgagtgcct tgggatcgtc ggagaacccg cgacccgaca cggtcgggcg
      241 cgacacggca cggcctgtgg tcgagtcgac cgccaccgtg atcgagatga agccgccctc
      301 acccagcacg agccggtcgc ccagcgtgct ctcgccgatg tcaccgacgg cgagcccgtc
      361 gacgtacacg cgccccacct cgacccgccc ggtgatcgac gccctgccgt cgacgagatc
      421 gacgacgacg ccgtcctccg cgagcaccac ggagtcctcc ggcaccccgg ttctggtcgc
      481 cagcacggcg ttcgcccgca gatggcgcca ctcgccgtgc accggcatca cgttgctcgg
      541 ccgcaccgcg ttgtagagga acaggagctc tccggcaggc gagtgcccgg agacgtgcac
      601 tttcgcggtg ccctggtgga cgacggacgc gcccagccgg gtgagcccgt tgatcaccgc
      661 gtagaccgcg gtctcgttgc ccgggatcag cgagctggca aggatcacgg tgtcctcggg
      721 cttgatgctc accgagcggt ggtcgccgcg ggccatgcgg gccagcgccg agagcggttc
      781 gccttgcgac cccgtggaga cgatcacgag cttgtcgtcg ggaagctcca tcgcctcgtc
      841 gagctcgatg agcagcccct cgggcacgtt gaggaagccc agctccgcgg ccaagcccat
      901 gttgcgcacc atcgaccggc cgacgaaaca caccttgcgg ccgtgctcgg cggcggcgtc
      961 gagcacctgt tggacgcggt gcacgtggct cgcgaagcag gcgacgatga tccgcgcctc
     1021 ggcccggtgg aagatcccgt cgagcaccgg gccgatctcc cgctcggcga tcacgaaccc
     1081 ggggatgtcg gcgttcgtgg agtcgacgag gaacaggtcc acgccctcgt cgccgagccg
     1141 ggagaagccc gccaggtcgg tgagcctgcc gtcgagcggg agctggtcaa gcttgatgtc
     1201 accggtgtgc agcaccaggc ccgccggcgt gcggatcgca acggccagcg catcggggat
     1261 cgagtggttg accgcgaagt actcgcagtc gaacgagccg tgccgcagcc gcgcgccctc
     1321 cttcacctcg agcaggtgcg gggtgagccg gtgctccttg cacttggcgg ccaccagcgc
     1381 gagcgtgaac cgcgacccga cgacggtcag atccggccgc tggcgcagca ggaacggcac
     1441 cgcgccgatg tggtcctcgt ggccgtgggt gagcaccagc gcgtcgatgt cgtcgaggcg
     1501 gtcctcaatg gcccggaagt cgggcaggat caggtcgacg ccgggcgagt cctcggcggg
     1561 gaacaacacc ccgcagtcga cgacgagcag cctgccgtcg tactcgaaga ccgtcatgtt
     1621 gcggccgatc tcgccgatgc cgccgagcgc gaccacgcgc agggcgccgt cgggcagcgg
     1681 cggcgggggg ccttgcggga gctcggccgg gttgacctcc tccggcggag cgggcacggg
     1741 acggccgtgg tcgcgccggg gcggccggga acgtcgatcg gaccgggggc ggtcggaccg
     1801 agctgggggg cgactcaact agtttcctcg cagtagtggt aggagcgatg gtgctctctc
     1861 agcggtaggc gacctcgaca ccgagctcgg ccgaagaggc gcccagcgcc ccgtggccgc
     1921 gatcggtgcg gcggtacgca tcgggatcga gagcgatccc cgcggcgccc aggtcgccgg
     1981 cgatggccgc cacctgctcc tcggtggccg gaggcagcgg cagccggggg cacccgacgt
     2041 cgaggccacg cagcccgaga gcggccttcg cgaacaccac cccgccgacg cggcccatcg
     2101 cgcggatcac cggcagggcg cttgcgttca gtgtgcgggc ccggtcgacc tcgccggcct
     2161 cgtaggcgtc gagcatctcg cgcagccggt tggccacgac gtgcccgatc acgctgacga
     2221 acccgaccgc gccgaccgac agccacggca ggttcaccgg gtcgtcgccc gagtagtagg
     2281 ccaggggtgt ggtggcgagg acctcggtac cgacccggag gtcgttgcgg gcgtccttca
     2341 cggccacgat ccgcggatgc tcggcgagcc gctgcagcgt ctcgatctcg atcgggatca
     2401 cgctgcgcgg cggaatgtcg tagagcatca ccggcaggtc ggtggcatcg gcgatcgccg
     2461 tgaaatgcag caccaacccc gcctgcggcg gtctcgagta gtagggcgtg acgatcagca
     2521 gcccgtgcgc gcccgccttc tccgctgcgc gggagagctc gatgctgtgt gcggtgtcgt
     2581 aggtacctgc ccctgcgacg atcgtggccc ggttgcccac ggcgctcacc acggcacgca
     2641 ccaggtcgga cttctcccgg tcggtcgtgg tgggcccctc ccctgtggtg ccattgagca
     2701 ccagcccgtc gttgccgagg tcgatgaggt gggcggcaac ttcctcggcc ttgtcgaggt
     2761 ccaggtggcc atcggcgtcg aagggtgtga ccatcgcggt cagcacccgg ccgaacggtc
     2821 ggcccgacgg gcgcggccca gcaggggtgg gactcatggg gtgacggtac cgcccggagg
     2881 acggcgggtg ctccccagcg ccgtgccggg cgcatcgaaa tcaaccgcag ggggagcgat
     2941 tccgtgcagg ggcggccggt ggcgggggca tcggtcctcg tcgactccgc tgggaagcag
     3001 gacgaaccgg tcacccgacc gccgccgaac gtgccggccc gtctccgcgc gctacacgcc
     3061 ccgagcggcg gcggccaccg cctcgacggc cgtggggtcg ccctccagct cgacgcgcgc
     3121 tgcgtcgcgc ccgaacgcat gcaggacgag ctcagcgggc tcgcccacga tcgtcaccag
     3181 gccggcgccg gtcctgacga cgtgctggtc acccgagggc cgccgcagga cgatgcccac
     3241 cgggctgtgc cgatagagcc cactccccat gcggaccacg gcggcccaga gcttcgcatc
     3301 gcgcaccggg tcgggcggcc gtggctccca gcccggctcg ccacggcgga cgtcctcgtg
     3361 gtggacgaag aactcgatgc cgttgccgac ctcgtcgagc ttggggatcc ggaacggcga
     3421 ccacagcggc gggccggccc gcagctgccc caccatgtca ccccacgggg cgccctcgta
     3481 gccgcccatc gcccgctcgg tgaacggcgc cagcgcgggc accacgatcc cgggcgccgc
     3541 ccacggctgc cgctcgcgga ccaggaggtg ggcgagcaga tggcgcgtgc tccagccctc
     3601 gcacagggtg ggacggtcgg gccccacccg ctccagctcg tcgcagatcg ccgcacgctc
     3661 gctgctcgcc agggtcatcg ctcgccctcg ctgcgctcgg ccggcccggc gtgagacgtc
     3721 tcagcgtgcg ggcgctgtgt ccatgattcg ctcccgaggt ctctcatcgc gccgacccta
     3781 gcgacggctc ggtccgcgcc gcattgcacg ggccgaccga tgacgccacg gcgtggtgat
     3841 cggaaccggg cagcgcgacc cgtcgtgggg cgtcaccgca ccaccccgtg ctgacgagga
     3901 catggtcgat gcgcaggagc aacggcgccc actggccggt ggacgtgtac ccgccccacc
     3961 cgtcgcgcat cgcgtcgacg agcccgccgt cgcgcaggat ctcccggtag tcgcgccccc
     4021 ggtcgggggt gttgaggtcc ccggtgacca cgacgggcag cgcctccccg gccacgcgcc
     4081 cggcgagcgc tcgcatgatg ccgtggtgct ccgtgacagt ggcctggtag gcgccgacgg
     4141 tggtgaacca cggccgcggg acgtgcagcg cgtacaggac gaacggaccc gccggaccgg
     4201 ccacctccag ccgcagcccg ggcaggtcgg gagcctgcga ctcgaccagc cgcagcggca
     4261 gcctgctgta gacggccacc tccgggccgc cgaccgcccg ctcgcggtac gggtaggcgg
     4321 gcaccaggac gggggccagg tcgtcggtcg cctccgacac gaccagcacg tcggcctgca
     4381 gcgcgaggag ctcgtcggca gactcgggcg agcccatcac attggcggcc accaccgtca
     4441 ctgcgcgacc tggcgccacc cgacccgcgt ccgacggcag ccacggcccg acgaccgttg
     4501 cgcaacccag cacgagcacg gacgccgcgg gcgccagcca ccgccgcctg cgcaggcgca
     4561 ccgcgccgac gacggccgca gtggcgatca gcgccaccac cgctgggagc acgatcgcga
     4621 gcacgtcggt gaccggcccg atggcgtcac gcacggcgaa ccaggcccac ggcaacgccg
     4681 cggacagcgc cacgggagcg aggtaggcca cccgagcgag acgggcgacc agggcgacgc
     4741 ggacggacgt gcgctgccac cgaccgcggg cgtgctccgt gcggccggct cgcgccggtc
     4801 tcacccctcg gtgacgaagg ggctcgacgc gatctcggtg ccgtcctcca acgtcgcgat
     4861 ctcgaagtcg ccgaacacgt tgggcacctc gcgctgcagc tggcgcaggc actcgatggc
     4921 gagctcgcgg atctcgacat cggcgtgctc ggtggcccgc atggcgatga agtgccgcca
     4981 cgctcggtag ttgccggtga cgacgatgcg cgtctcggtg gcgttgggca ggaccgagcg
     5041 tgcggcctgc cgcgcctgct tgcgccgcag cgtggcgttg gggacgtcgg cgaacctctt
     5101 ctccagccct tcgagcagcg cctcgtaggc cgtgaccgac gcctgcgcgg cctcggtgaa
     5161 caggttgtgc agctcgggat cggctgcgat gacgtcgggc tcgaccatgg ccgcgtcgcg
     5221 ctccgggacg taacgctggg agagctggct gtaggagaag tgccggtggc ggatgagctc
     5281 gtgggtgagc gagcgcgaga ccccggtgag gtagaagctc accgagccgt gctcgagcac
     5341 ggacaggtgc ccgacctgga ggatgtgccg caggtacccg gcgttggtcg cggtggacgg
     5401 gttgggcttg gcccacgact ggtaacacgc gcggccggcg aactccgcga gcgcctggcc
     5461 cccgtccgcg tccgtgctcc acggcacgcc ctcgggcgcg acgaactcgg tcttggcgac
     5521 gagctggacg cccagcggaa cgatctgagg catgacggca gcgtaaccgg gcaccccgac
     5581 ggaaccggca tgacaccaga tgacaccatc gggctccttg actgatgtca cgattgacgc
     5641 cacagtgatg tcatggacct cagccagtac gtcacgcagc tgcgggagga cctcgcctcc
     5701 gcagccgctg ccggcgacga gcagacgcgg cgcaccgccg cgctgctcgg tgccgccatc
     5761 gagccggctg cacgactggc tctcatgaac gcgctctccg atctcgcggc cgaggtcacc
     5821 gccgcgctac cggaccgcgt ggtcgaggtg cgcctcgacg gccgcgacgt gcgtgtcgcg
     5881 gtgaccgccg acccggccgc cgaaccggcc gcggaggagc ccccaccccc gccgttccgc
     5941 gggttcccgc cgggcgccga cgcgggggac atcagccgga tcacgctgcg gctgatggag
     6001 cagatgaaga accaggcaga acaggccgcc gccgcccagg gcgtgtcgct caactcgtgg
     6061 ctggcgcagg cggtgcaggg ggcactggcc ggcggcggac ggcgtggccg ccggaactgg
     6121 acacagggtg accgcgacga acggcgccta cgcggatggg tcgaggggtg agcgacatgg
     6181 tggacttcgc gaaggacgag cccggcaccg c
//
LOCUS       DASRSC010000217         8075 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1716998,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000217 DASRSC010000000
VERSION     DASRSC010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8075
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1716998"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..2091
                     /locus_tag="VFE39_10625"
     CDS             61..2091
                     /locus_tag="VFE39_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6259094.1"
                     /translation="MTETPGTTFHDLQAYVALPRLTGLALSPDGSRLVTTVAALGPEK
                     NSYRPALWEIDPTGEQPARRLTRGPTGESSPAFTPDGDLLFTSARPDPDAKEQPSDAP
                     QALWRLPAGGEAHIVGTRPGGVGGPVVARDTGTVVVSSMTLPAAVTADDDEKRRKERR
                     EKKVSAILHFGYPVRLWDHDLGPDEPRLVTGAVREDGPVEWRDLTPAVGGALVDVAHD
                     VSPDGATVVTAWQVAEPGGSSRSTLVAIDVATGERQVLAADPTHEFGGPVISPDGTQV
                     ACWQESISTPTNPPATRLVVVPLAGGEARDVAPGWDRWPGDVRWTPDSSALIVVADDA
                     GRAPVFRIELADATVTRLTGDDGAYSDVRVAPDGVHVYALRSAVDHPPAPVRLDARTP
                     DQEPVLLPGPARSPVLPGTLTEVHATAADGSPLRAWLVLPDGTAESSPAPLLLWVHGG
                     PLGSWNAWQWRWNPWLMAARGYAVLLPDPALSTGYGLDFVARGWGQWGGAPYTDLMAI
                     TDAAVARPDIDAERTAAMGGSFGGYMANWIAGHTDRFRAIVTHASLWALDQFGPTTDA
                     SYYWTREMTPEMAEHNSPHRHADAVTTPMLVIHGDKDYRVPIGEGLRLWWDLVSRHDG
                     DPAALPHRFLYFPDENHWVLSPQNAAVWYQTVTAFLDHHLLGKDWETPDLLR"
     gene            2301..3521
                     /gene="fdhA"
                     /locus_tag="VFE39_10630"
     CDS             2301..3521
                     /gene="fdhA"
                     /locus_tag="VFE39_10630"
                     /EC_number="1.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016697413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018467 - formaldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde dehydrogenase,
                     glutathione-independent"
                     /protein_id="HET6259095.1"
                     /translation="MGGNKIVVYKEPGVVAVEDHDIPKLEVPADVAKAKGLTQKAPHG
                     VILRNVATNICGSDQHMVRGRTTAPPGQTLGHEIMGEIVEKGEDVQFLDIGDICSVPF
                     NIACGRCRNCKEGQTGVCLNVNPARAGSAYGYVDMGGWEGGQAQYVMVPFADFNLLKI
                     PDREQALEKVLDLTMLSDIFPTGYHGAYTAGVTTGSTVYVAGAGPVGLAAAYSSQLLG
                     AAVVIVGDRNPDRLAQARSFGCETVDITADASLEDQIAQILGVPEVDSAVDAVGFEAS
                     GSGKGAGEAPAAVLNSIMAITRAGGKLGIPGLYVTGDPGAPDEDAKEGTLKVRLGLGW
                     AKSHSFTTGQCPVLKYNRQLMMAILHDKAHIADAVNATVIPLDEAPRGYQEFDQGAAR
                     KYVLDPNGMIAQAS"
     gene            complement(3535..4962)
                     /locus_tag="VFE39_10635"
     CDS             complement(3535..4962)
                     /locus_tag="VFE39_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6259096.1"
                     /translation="MSASLHVLVVGGGMTGLSTALLLAQDGHRVTVLERDAASPPEPE
                     TAWEEWDRRGCAQFHLPHYALPPFRRLLEAELPAVSAALLAAGAAPYDVVGELPEAIT
                     GGRRDGDDRYVALASRRPVLEAVVARAAEAQPGVEVRRGVVVRGLLTDTPDRTGVPHV
                     LGVVTGDGEQVRADLVVDAGGRRSAMPALLAAAGGAAPAEEREDSGFVYYARYFRADG
                     RPALQGQVHQFYNSVSVLTLPSDRGTWSVTFAAGSRDIELHVLREPDRWMAVARCFPA
                     SAAWIDAEPVTDVKVIAGIEDRIRHYVLDGRPVASGLLPVGDAWACTNPSLGRGMSMA
                     LMHAVLLRDVLRAGSGDRPAELSVRWYEATGEVLEPWYRATVANDRHRLAEVEADRSG
                     VPYVPADRGWAMAKALQAGSRRDPDVLRALLDIASMEMLPPEVFGRPGMVERVLAAGA
                     GAPQYAMPGPDRRGLLAAAGVAVPV"
     gene            complement(5065..5988)
                     /locus_tag="VFE39_10640"
     CDS             complement(5065..5988)
                     /locus_tag="VFE39_10640"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HET6259097.1"
                     /translation="MPPAPGFRAAVQRARATVRAYLGLTKTRIIEQLLVVTVPAMFLA
                     QRGVPSVWLIIATLVGGAAAAASAHALNCVVDADIDAVMKRTARRPLAKGQVPPRNAL
                     VFGLALGVLSAVWLGLTTNWLAAGLSVAAIAFYVLVYTLLLKRRTSQNIVWGGAAGCM
                     PVVIGWAAVTGTVQWPALVMFGVIFFWTPPHFWALAMRYRDDYAAAGVPMLPVVATPV
                     QVSRRILAYSWVMAAWTLLLVPATSWIYAAVAVLGGAWFVFQAHRLHAAVAAGRDVTP
                     MKLFHLSNMYLCVLFAAIAVDAAVGLPVLRP"
     gene            6243..>8075
                     /gene="tkt"
                     /locus_tag="VFE39_10645"
     CDS             6243..>8075
                     /gene="tkt"
                     /locus_tag="VFE39_10645"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET6259098.1"
                     /translation="MADTDIETLTRANVPADWSDLDKRTVDTIRVLAADAVQKVGNGH
                     PGTAMSLAPLAYLLFQRVMRHDPTDPDWIGRDRFVLSAGHASLILYIQLYLSGYGLEL
                     EDLEALRTWGSKTPGHPEHRHTRGVEITTGPLGQGFASAVGMAIAARRERGLFDPEPG
                     TGESPFDHDIYVIASDGDIEEGVTSEASSLAGRQQLGNLTVIYDDNQISIEDDTNIAL
                     SEDTAKRYEAYGWHVQVVEGGENVTGILAALEAAKAETSRPSFIVLRTVIGYPAPNKM
                     NTGKAHGSALGDEEVAEVKKILGFDPDKSFQVEDEVLAHARKVVDRGRAAHEAWNTQF
                     EDWAAREPERKDLLERLLGRDLPIGWEKALPSWEPDPKGVATRKASGEVLKSLADVLP
                     ELWGGSADLAESNNTTMEGADSFGPESAATKMWDAHPYGRTLHFGVREHAMGAILNGI
                     ALHGPTRPYGGTFLTFSDYMRPAVRLAALMQSSVIYVWTHDSIGLGEDGPTHQPIEHF
                     AALRAIPGLAFVRPGDANETAHAWRATLERPEGPKGLALTRQNLPVLEGTSAEGVARG
                     GYVLAEASSGKPQVILMATGSELQIAVEAREVLEADGVPTRVVSM"
BASE COUNT         1299 a         2911 c         2828 g         1037 t
ORIGIN      
        1 cgtaggagtc acgtcgggta gaaccgggca gacttctcgc accgtgctag acatcaactc
       61 gtgacggaga ccccgggcac caccttccat gacctgcagg cgtatgtggc gctgccccgc
      121 ctcaccgggc tggcgctgtc ccccgacggc agccgactcg tcaccacggt ggcggcgctc
      181 ggcccggaga agaacagcta ccgcccggcg ctctgggaga tcgaccccac cggcgagcag
      241 cccgcgcgca ggctcacccg cggccccacc ggcgagtcct cccccgcctt cactcccgac
      301 ggcgacctgc tgttcacctc cgcccggccg gaccccgacg ccaaggaaca gccctccgat
      361 gcgccgcagg cactgtggcg actcccggcc ggcggcgagg ctcacatcgt gggcacccgg
      421 cccggcgggg tcggcggacc ggtggtcgca cgcgacaccg gcactgtcgt cgtctcgtcg
      481 atgacgctcc cggccgccgt gaccgccgac gacgacgaga agcgccgcaa ggaacggcgc
      541 gagaaaaagg tcagtgcgat cctgcacttc ggctaccccg tccggctctg ggaccacgac
      601 ctcggaccgg acgagccccg gctcgtcacg ggcgcagtgc gcgaggacgg tcccgtcgag
      661 tggcgcgacc tgacccccgc agtgggcgga gcgctcgtcg acgtggccca cgatgtctcc
      721 ccggacggtg ccacggtcgt caccgcgtgg caggtcgccg agcccggcgg gtcgtcgcgc
      781 agcacgctcg tggcgatcga cgtggcgacc ggggagcggc aggtgctcgc cgccgacccc
      841 acccacgagt tcggcgggcc ggtgatcagt cccgacggca cgcaggtggc gtgctggcag
      901 gaatccatct cgacgcccac gaacccaccc gcgacccggc tcgtggtggt gccgctggcc
      961 ggtggcgagg cgcgcgacgt ggcgcctggc tgggaccgct ggcccggcga cgtccggtgg
     1021 acgcctgact cttcggcact gatcgtcgtg gccgacgacg ccggccgcgc acccgtcttc
     1081 cgaatcgagc tcgccgacgc caccgtcact cgtctgaccg gcgacgacgg cgcctacagc
     1141 gacgtcaggg tggcaccgga cggggtgcac gtctacgcac tgcgcagcgc ggtcgaccac
     1201 ccgccggcac ccgtccggct cgacgcgcgc accccggatc aggagcccgt gctgctaccc
     1261 ggtccggccc ggtcccccgt gctgccgggc acgctcactg aggtgcacgc cacggccgcc
     1321 gacggcagcc cgctgcgtgc ctggctcgtg ctgcccgacg gcacggcgga gagctccccc
     1381 gctccgctgc tgctctgggt gcacggcggt ccgctcggta gctggaacgc ctggcagtgg
     1441 cgctggaacc cgtggctgat ggccgcccgt gggtatgccg ttctactgcc ggacccggcg
     1501 ctgtccaccg ggtacggcct cgacttcgtc gcgcgcggtt gggggcagtg gggcggagcg
     1561 ccctacacgg acctgatggc gatcaccgac gccgctgtcg cccggcccga catcgacgcg
     1621 gagcgcacag cagccatggg cggctccttc ggcgggtaca tggccaactg gatcgccggg
     1681 cacaccgacc gcttccgcgc gatcgtgacc cacgcgagtc tctgggcact cgaccagttc
     1741 gggcccacca ccgacgcctc ctactactgg acgcgcgaga tgacgccgga aatggcggag
     1801 cacaacagcc cgcaccgcca cgccgacgcc gtcaccacac ccatgctggt gatccacgga
     1861 gacaaggact accgggtgcc gatcggtgag ggcctgcgcc tgtggtggga cctggtctcc
     1921 cggcacgacg gcgatccggc cgcccttccg caccggttcc tgtacttccc cgacgagaac
     1981 cactgggtgc tgtcgccgca gaacgcagcg gtctggtacc agacggtcac ggcgttcctg
     2041 gaccaccacc tcctgggcaa ggactgggag acgcccgacc tcctgcgctg acccgatatg
     2101 cgggacgact cgctccgcga atgggtgatg cccgcgctcg gccaactgcc gggttttagc
     2161 tggtgaacgg cgtacgagct ggtccgatcg cggggaagca gggtccaggt gctgcctgcc
     2221 ccttgcgcgg gccctcgccc gcgagtaggt ctcggagggg agagtgagtc caagcccaaa
     2281 gaaaggtgtg gaggattcac atgggtggca acaagatcgt ggtttacaag gagcctggcg
     2341 tggtcgcggt cgaggaccac gacattccga agctcgaggt gcccgcagac gtggcgaagg
     2401 cgaaggggtt gacgcagaag gccccacatg gcgtgatttt gcgcaatgtc gccacgaaca
     2461 tctgcggctc cgatcagcac atggtgcgcg gtcgcaccac tgcccctccc ggccagacgc
     2521 tcggccacga gatcatgggg gagatcgtcg agaagggcga ggacgtccaa ttcctcgaca
     2581 tcggcgacat ctgctcggtg ccgttcaaca tcgcctgcgg acgctgccgc aactgcaagg
     2641 aaggccagac cggcgtctgc ctcaacgtga acccggcccg ggccgggtcg gcctacgggt
     2701 acgtcgacat gggcggctgg gagggcggcc aggcgcagta cgtcatggtg ccgttcgccg
     2761 acttcaacct gctgaagatc cccgaccggg agcaggcgct ggagaaggtt ctcgacctga
     2821 cgatgctgtc ggacatcttc cccaccggat atcacggcgc gtacaccgct ggtgtgacga
     2881 ccgggtcgac ggtctacgtc gcgggcgccg gaccggtcgg actcgccgcg gcgtactcgt
     2941 cgcagctgct cggcgctgcc gtggtcatcg tcggggaccg caaccccgac cggctcgcgc
     3001 aggcgcgctc gttcggctgt gagacggtgg acatcacggc ggacgcgtct ctcgaggacc
     3061 agatcgcaca gatcctcggg gtgcccgagg tggactcggc cgtcgacgcc gtgggcttcg
     3121 aggccagcgg gagcgggaag ggcgcggggg aagccccggc cgccgtcctc aactcgatca
     3181 tggccatcac gcgcgcgggc ggcaagctcg gcatccccgg cctctacgtc accggcgacc
     3241 ccggggcgcc tgacgaggac gcgaaggaag gcacgctcaa ggtccggctg gggctcggat
     3301 gggcgaagag ccattccttc acgacgggcc agtgcccggt gctgaagtac aaccgccagc
     3361 tgatgatggc catcctgcac gacaaggccc acatcgctga tgcggtcaac gcaacggtga
     3421 tccccctgga cgaggcgcct cgcggctacc aggagttcga ccagggcgcg gcgcgcaagt
     3481 acgtcctgga cccgaacggg atgatcgctc aggcgtcgta acgcctgatc cgggctacac
     3541 cggaacggcc acccctgctg cggcgagcag tccacggcgg tcggggccgg gcatcgcgta
     3601 ctgcggtgct ccggccccgg ccgccaggac acgctcgacc attcccgggc ggccgaacac
     3661 ctccggcggc agcatctcca tcgaggcgat gtcgagcagc gcgcgcagga cgtccgggtc
     3721 gcgccgggag cccgcctgca gagccttggc cattgcccag ccgcggtctg ccgggacgta
     3781 cgggacgccg gaccgatcgg cttcgacctc ggcgaggcgg tggcggtcgt ttgcgacggt
     3841 ggcgcggtac cagggctcga gcacctcgcc cgtcgcctcg taccaccgaa cggacagctc
     3901 agcagggcgg tcgccgctcc cggcccgcag cacgtcgcgc agcagcaccg cgtgcatcaa
     3961 cgccatcgac atcccgcgcc ccagggacgg gttggtgcac gcccaggcgt cacccacggg
     4021 aagcagcccg ctggcgaccg gacgcccgtc caggacgtag tgccggatgc ggtcctcgat
     4081 gcccgctatc accttcacgt cggtcaccgg ttccgcgtcg atccacgccg cgctcgccgg
     4141 aaagcaccgc gccaccgcca tccaccggtc gggctcacgc agaacatgca gctctatgtc
     4201 ccgcgagccg gcggcgaacg tgacgctcca ggtcccgcgg tccgacggga gggtgagcac
     4261 cgagaccgag ttgtagaact ggtggacctg accctgtagt gccgggcggc cgtccgcgcg
     4321 gaagtagcgg gcgtagtaga cgaaaccgct gtcctcgcgc tcctcggccg gggcggcgcc
     4381 accggccgcg gcgaggagag cgggcatggc cgatcgacgg ccacccgcgt cgaccaccag
     4441 gtcggcccgc acctgctcgc cgtcgccggt gaccaccccg aggacgtgcg gcacgcccgt
     4501 gcggtccggc gtatccgtca gtaggccccg cacgaccacc ccgcggcgga cctccacccc
     4561 cggctgcgcc tccgcagccc gggcgacgac ggcctcgagc accgggcgcc gggaagcgag
     4621 cgcgacgtag cggtcgtccc cgtcgcgccg gccccccgtg atcgcctcgg gcagctcgcc
     4681 cacgacgtcg tacggcgcgg cgcccgcggc gaggagggcg gcggagacgg caggcagctc
     4741 ggcctccagc aggcgccgga acggcggaag ggcgtagtgc ggcagatgga actgggcgca
     4801 gccgcgccga tcccactcct cccacgccgt ctcgggctcg ggagggctcg ccgcgtcgcg
     4861 ttccaggacg gtcacgcggt ggccgtcctg ggccagaagc agtgccgtgg acaggccggt
     4921 catgccgccg ccgacgacaa ggacatgcag ggacgccgac accgatacct ccagaggggg
     4981 tgcctggggc gggaacgccc ggagttcgag gtcggacagc cacgatgacc tctgcggcga
     5041 ggctatcggc gaaccgcggg gtgctcaggg gcgcaggacc gggaggccca ccgcggcgtc
     5101 caccgcgatc gcggcgaaga ggacgcagag atacatgttg gacagatgga agagcttcat
     5161 cggggtgacg tcgcggcccg cggcgacagc ggcgtggagc cggtgggcct ggaacacgaa
     5221 ccaggcacct ccgagcaccg ccacggcggc gtagatccag gaggtggcgg ggacgagcag
     5281 cagcgtccac gcggccatca cccacgagta cgcgaggatg cgccgcgaca cctgcaccgg
     5341 cgtggcgacg acggggagca tcggcacgcc ggcggccgcg tagtcgtcgc ggtagcgcat
     5401 ggcgagtgcc cagaagtgcg ggggcgtcca gaagaagatc acaccgaaca tcaccagcgc
     5461 cggccactgc acagtgccgg tcacggcagc ccagccgatc accacgggca tgcagcccgc
     5521 agcaccgccc cacacgatgt tctgtgaggt gcgtcgcttg agcagcagtg tgtacacgag
     5581 cacgtagaag gcgatcgcgg ccactgacag ccccgcggcc agccagttcg tggtgagccc
     5641 gagccacacg gcgctgagca cccccagcgc gagaccaaag accagcgcgt tgcgcggagg
     5701 aacctggccc ttggcgagcg gacgcctcgc ggtgcgcttc atcaccgcgt cgatgtcagc
     5761 gtcgaccacg cagttgaggg cgtgagcgct cgccgcggcc gccgcaccgc cgacgagcgt
     5821 ggcgatgatc agccacaccg agggcacccc gcgctgggcg aggaacatcg ccggcacggt
     5881 gacgacgagc aactgctcga tgatgcgggt cttcgtgagc ccgaggtacg cccgcacggt
     5941 ggcgcgcgcc cgctgcaccg cggcccggaa gcccggagca gggggcacct gcgacgcccc
     6001 ctggtgggac acgggcacac tcaccggcaa cctcaatcca cgtggtagtg gggctcagac
     6061 gcacccagtt ctacggcctg tcgatggtaa cggggcgcat gtcgggaggc gcttccgggt
     6121 agacccgggt gaccgccgag ccagtgatca caggtggcta cgccaggtcg gtaggctgac
     6181 gcgcaagccg gatgcgtcga ggcgttccgc ctgcgccccc accagaaacc tagggaagtg
     6241 ccgtggctga caccgacatc gagactctga cccgagccaa cgtgcccgcc gactggagcg
     6301 atctggacaa gcggacagtc gacaccatca gagtgctcgc cgccgacgcc gtgcagaagg
     6361 tgggcaacgg gcaccccggc acggcgatga gcctggcgcc gctggcgtac ctgctgttcc
     6421 agcgggtcat gcggcacgac ccgaccgacc ccgactggat cggccgcgac cgcttcgtac
     6481 tttcggccgg ccacgccagc ctgatcctct acatccagct ctatctctcc ggctacggcc
     6541 tggagctcga ggacctcgag gccctgcgca cctggggctc caagaccccc ggccaccccg
     6601 agcaccggca cacccgcggg gtggagatca ccaccgggcc gctcggtcag ggcttcgcct
     6661 ccgccgtcgg catggcgatc gcggcccgcc gggagcgcgg tctgttcgac ccggaaccgg
     6721 gcaccggaga gagcccgttc gaccacgaca tctacgtcat cgcctccgac ggcgacatcg
     6781 aggagggcgt cacctccgag gcgtcgtcgc tggccggccg ccagcagctc ggcaacctca
     6841 ccgtgatcta cgacgacaac cagatctcga tcgaggacga caccaacatc gcgctctcgg
     6901 aagacaccgc gaagcgctac gaggcctacg gctggcacgt gcaggtcgtc gagggcgggg
     6961 agaacgtcac cggcatcctc gccgccctcg aggccgcgaa ggccgagacg agccgcccct
     7021 cgttcatcgt gctgcgcacc gtcatcggtt accccgcgcc gaacaagatg aacaccggca
     7081 aggcgcacgg ctccgccctg ggtgacgagg aggtggccga ggtcaagaag atcctcggtt
     7141 tcgaccccga caagtcgttt caggtcgagg acgaggtgct cgcgcacgcc cggaaggtcg
     7201 tcgatcgcgg acgtgcggcc cacgaggcct ggaacacgca gttcgaggac tgggccgccc
     7261 gtgagccgga gcgcaaggac ctgctcgagc ggctgctcgg ccgcgacctc ccgatcggct
     7321 gggagaaggc gctgcccagc tgggagcccg accccaaggg ggtcgcgacg cgcaaggcgt
     7381 cgggtgaggt gctcaagtcc ctcgccgacg tgctgcccga gctgtggggc ggctcggccg
     7441 acctcgcgga gagcaacaac accaccatgg agggtgccga ctcgttcggc cccgagtccg
     7501 cagccacgaa gatgtgggac gcccacccct acggccgcac gctgcacttc ggtgtgcgcg
     7561 agcacgcgat gggggccatc ctcaacggca tcgcgctgca cggcccgaca cgcccgtacg
     7621 gcggcacgtt cctcacgttc agcgactaca tgcgcccggc cgtccggctc gcggcgctga
     7681 tgcagtcgag cgtcatctac gtgtggacgc acgactccat cggtctgggc gaggacggcc
     7741 cgacccacca gccgatcgag cacttcgccg cgctgcgcgc gatccccggg ctcgcgttcg
     7801 tccggcccgg cgacgccaac gagaccgccc acgcgtggcg ggccacgctg gagcggccgg
     7861 aggggccgaa gggtctcgcg cttacccggc agaacctgcc cgtgctggag ggcacgtcgg
     7921 ccgagggcgt cgcccgcggg ggctacgtgc tggcggaggc gtcgtccggc aagccgcagg
     7981 tgatcctcat ggccaccggg tccgagctac agatcgccgt ggaggcccgc gaggtcctcg
     8041 aggccgacgg cgtgcccacg cgggtggtgt cgatg
//
LOCUS       DASRSC010000218        12798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3028259,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000218 DASRSC010000000
VERSION     DASRSC010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12798
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3028259"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..728
                     /locus_tag="VFE39_10650"
     CDS             234..728
                     /locus_tag="VFE39_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6259099.1"
                     /translation="MGRSVLVTGGNRGIGLAIAKAFAAQGDQVAVTHGSPVEGLPGEL
                     LPVQCDVTDTAAVDAAFTEIEDKHGAVQVLVSNAGITDDGLLMRMTEESFTRVVAPGF
                     VDTDMTRALPDARRTEILGQVPLGRYASPYEIAAAVTWLGSESAGYIAGAVIPVDGGL
                     GMGH"
     gene            760..1551
                     /gene="fabI"
                     /locus_tag="VFE39_10655"
     CDS             760..1551
                     /gene="fabI"
                     /locus_tag="VFE39_10655"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HET6259100.1"
                     /translation="MGLLEGKRLLITGVITDASLAFHAAKIAQEQGADVVLTGFGRMR
                     LVERIAQRLPKPAPVVELDVSDQSQLDSLVERVSPHLAGANGEEEPKLDGVLHSIGFA
                     PASCLGENAFLNAPWEDVATTIQVSAYSFKSLSTAVLPLLGPGSSIVGMDFDNRQAWP
                     AYDWMGVAKSTLESVTRYLARELGPKGIRVNLVAAGPVRTMAAKSIPGFAAFEDAWDG
                     RAPLGWDVNDPVPVAKTVCALFSDWLPVTTGSMVWADGGFHAIGV"
     gene            1559..2605
                     /locus_tag="VFE39_10660"
     CDS             1559..2605
                     /locus_tag="VFE39_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET6259101.1"
                     /translation="MRKQVDALLVLSFGGPEGPEDVRPFLENVVRGRGIPPERLDAVE
                     EHYRHFGGVSPLNARNRDLIAAIRQRTDLPVYFGNRNWHPMVEDTVAEMMRDGVRRAL
                     VFATSAYGGYSACRQYQEDIVRARKTVGDGAPELVKLRHFFDHPEFVAANADAVRAAG
                     ASLPADLRDDARLVFTAHSVPVAVDAAGGPPEEGGHRYSRQIAEAARLVAAELGVAEY
                     DLVWQSRSGPPEVPWLEPDIVDHIDALRDRGVAAVVVAPVGFVSDHVEVVWDLDTEAR
                     ERATELGMGFARAATAGPDPRFADMVVELVAEHVHDAAPRSLGAVPSAGCTVNGAPCA
                     VDCCAPAQRPSRPE"
     gene            complement(2768..2914)
                     /locus_tag="VFE39_10665"
     CDS             complement(2768..2914)
                     /locus_tag="VFE39_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259102.1"
                     /translation="MTASKQFLTSASDAWGAENTSAGTDPAAARAAADRAAAFYYTGV
                     PEQA"
     gene            complement(3221..3370)
                     /locus_tag="VFE39_10670"
     CDS             complement(3221..3370)
                     /locus_tag="VFE39_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259103.1"
                     /translation="MDDQSEHRQVEQDHSRAVTGQARAEVEPDVGIEFCNDVATDPTA
                     TVRTL"
     gene            complement(3527..4729)
                     /locus_tag="VFE39_10675"
     CDS             complement(3527..4729)
                     /locus_tag="VFE39_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET6259104.1"
                     /translation="MAASEPLAILEALAGHRSPTAPVPVTPGVSDWPGVDDPEGLRAQ
                     LVDWLGQYANAGTRRTYAYALGLPIAWVDAIGGAPPAGSGQAAPPPAQAGPLHDLAWF
                     RWCARQAIDPRDATARHVKAWLHALDAAGAQRRTRQRMLSTLSALYGHLAETGVVAAN
                     PAALNRARLGLSSTARDASPTVRLTAEQLKALLVAAARLPYSRSPRLARLYALRAVAM
                     VALLTLGLRISEVVGLDRGDLYRTGGDDVLRVLGKGGLHREVYLTDLVRDALTAYLAE
                     RDRVGDAALPARRGRTGAGGTPLLATRNGGRCSRVDLYTLLRRIAAHAGPVLDGLADR
                     VHPHALRHAYVTIALEQDARIQHVQADVGHATIATTQHYDRGRRTRASTAADLVAAAI
                     AAAPDPMP"
     gene            complement(5160..5942)
                     /locus_tag="VFE39_10680"
     CDS             complement(5160..5942)
                     /locus_tag="VFE39_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6259105.1"
                     /translation="MTDSGSPLVQITGVTKSFDAVRALQGVDLSIDKAEVTALLGDNG
                     AGKSTLVRCLTGVHPPDRGEIRFRGEPIRLTSPDDARRLGIETVYQTLGLIEDLQVWQ
                     NLYLNRELTTGFGPFRVVDRKAMIASSKEILSRLDVDVPNVRATVRRMSGGQRQSIAI
                     ARAANWGETLVIMDEPTAALGVRETAAVEELIRRLRESGVTVLLVSHDMGQVMRVADT
                     AYVLRRGRTAARRSIPDTTGDELVGLITGAIAGDEAGRDAVA"
     gene            complement(5953..6915)
                     /locus_tag="VFE39_10685"
     CDS             complement(5953..6915)
                     /locus_tag="VFE39_10685"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6259106.1"
                     /translation="MTDLAVPPRNERRRFSMGDAARYAAPIGLVVVFVAFFIATPDFL
                     TVGNISDLLVSAAILLVLAMGQQLVVTVAGIDLSVGSNLPWAAAVLGFAYSHGWGLAV
                     SILAAVLAGMLVGVVNGVLVAKLSMTDFIVTLGSLSVVSGITLLLTGGNTVSVNSSFL
                     QGLALDGIGPLRWFWVVAALVALVVGFLLFRTRTGTYFLATGGNIEAARESGINVSRI
                     RLLAYAGSGLACGLASVLLVARTGGADPSLQTDLLLSSIAAVVLGGSSLFGGRASVLG
                     SAAGAVLLQSLINGFTLIGISQYYQPIAVGVVVLGAAFISRFQK"
     gene            complement(6922..7956)
                     /locus_tag="VFE39_10690"
     CDS             complement(6922..7956)
                     /locus_tag="VFE39_10690"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6259107.1"
                     /translation="MNTAMTPLRTALVLCAAATICTGLVACGRGEQAASGGGSGNVRI
                     AIVTRDFTNPYWAALRDGAVAEGKAQGVTVDVQAGSSETDSTGENAQISTLAGQNVSC
                     FGVVPVNATNVITPLIPVARKKIPILNLDTQIDPNASAQAGVSYASFIGSDNLSAGEQ
                     AGKALMAKMGGTGKVAILQGIAGEQNGINRQQGFEKATAGALQVVATQPADYDQAKAQ
                     TVTDAILKAHPDITGIFAANDTMGLGAAQSVRNAGLTGKVSIISVDGITAALQAVKDG
                     TLTGTISQYPYAEGQMAVQACMKLVQKQTVPERIVAPIQLIDASNADKAIASFPQPIA
                     PFDNPVAGGK"
     gene            complement(7993..9036)
                     /locus_tag="VFE39_10695"
     CDS             complement(7993..9036)
                     /locus_tag="VFE39_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6259108.1"
                     /translation="MAVRMRDIARLAGVSEATVSLALSGNSRVSEATRRRVIAIADEL
                     GYRPNAAARSLRTDSTRALGLVVSDVANPFFAELAGRIERIAAAQGYSVVLCNSDEDA
                     ARQDGYLSRLLAGSEVDGVMLVPAGGMTPGILAAGAARARMVLLDRPIAADGVGGVSE
                     WLRAIPVVRGSGAAALGEVAELLIGLGHRRIGIIALPQTTQIGRERHDMMLGALTGQG
                     LAASAVEVVSGDFRQHSGELAVERLLASPNPPTAYVAADGLMAIGAMKALRRAGLRIP
                     RDVSLVGYDDAPWFDLLDPPLTTVAQPVVELAEAAMRSMYALLAGETPPTTALTCQLV
                     RRSSCGPPPDAHS"
     gene            9969..11018
                     /locus_tag="VFE39_10700"
     CDS             9969..11018
                     /locus_tag="VFE39_10700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver protein"
                     /protein_id="HET6259109.1"
                     /translation="MIDEALRCGYSKCRAELPPPGPQGGRRRAFCRDTRWPGGRTCAQ
                     MARAERDALDALGLDSGRATFRLDADRLREHVEAVREPVAELAGALSAVSARLDEVEG
                     SALAAVEAANRGAAEAEAARVDAQQARERAERESREARDRAARAGEERAAAVDRAAAA
                     ARQALEATEALGAARQVAEEATAARQAAEDRAVAAARQVGEAERATQEAAVRAGRADA
                     ERAAAQARAQEWQAESEERRVELDAVRSQLAAVANARDDTARRASAAAAALAEEREGR
                     QRADQELAGARERAGVEMALRTRADGELERLRTEVERAHADARLARAELTALRERGTD
                     ELRALVTAAVQGKAL"
     gene            11056..11895
                     /locus_tag="VFE39_10705"
     CDS             11056..11895
                     /locus_tag="VFE39_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET6259110.1"
                     /translation="MTSPLRWFITGASTGFGRTFAEVLLAQGDHVVATARDKGAVADL
                     VERYPDAALALPLDVTDPEQVREAVAAATEAGPVDVLVNNAGHGLLGALEELSDEQIQ
                     QVLGVNLLGALAVTRAVLPHMRARRSGHIVQMSSVGGVVANPGHAVYATTKFALEGAS
                     EALAGEVAPFGIHVLIVEPGPFRTDFLGRSLEMATPIADYQGGPAGALRARFATQHGL
                     QPGDPVLAIETIIGVVRAGSAPLRLPLGHAAVQRIREKLQRQLDDLEAYADLSMSTDR
                     PVG"
     gene            11972..>12798
                     /locus_tag="VFE39_10710"
     CDS             11972..>12798
                     /locus_tag="VFE39_10710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET6259111.1"
                     /translation="MRIGLGLPHYGALATVDALLQVSRAAEAMGYDSLWTGDRLFVPV
                     RPSNPYPDASGVIPPQFATYMDPLTALTFAAAHTERVRLGMSTLNVLWYPPVVLARTL
                     TTLDILSRGRVDAGFGIGWMRDEYTAVNVPWERRGARLDETLDLLEKIWTSELVHHDG
                     PLFTVVPTPIEPKPVQRPRPPILLPGFTPQAMRRVGRRADGWLAVGAPLSGLGYLWST
                     IRREAEDAGRDPAEIRFIVRMNPHLTDVKADTEKVPSKGTMDQVIDYARCLVEVGAD"
BASE COUNT         1829 a         4503 c         4637 g         1829 t
ORIGIN      
        1 cgaaaccttg atctcgcggc gatcaacgac ccgcgcgacc ggaaagggcg actcgtggac
       61 gggaaaccgg cgactcgcgg gggactcgcg ggcgggacaa gccggcgacg cgcggacggg
      121 cgactcgggg gcgggaacgg gcgactcgcg gggtggtgcc gcgcgagtcg ccccaatccg
      181 cgcgcgagtc gcccggttta cagcggtggg ccgccggacc atagggtggc cccgtgggac
      241 gcagcgtgct ggtgaccgga ggcaaccgcg gtatcgggct ggcgatcgcc aaggcgttcg
      301 cggcgcaggg cgaccaggtg gcggtgaccc acggcagccc cgtcgagggc ctgccgggcg
      361 aactgctgcc cgtgcagtgc gacgtcaccg acactgcggc cgtcgacgcc gcgttcaccg
      421 agatcgagga caagcacggc gcggtgcagg tgctggtctc gaatgcggga atcactgacg
      481 acgggctgct catgcggatg accgaggagt cgttcacgcg cgtcgtggct cccgggttcg
      541 tcgacaccga catgacccgc gccctccctg acgcccgtcg taccgagatc ctcggccagg
      601 tgccgctcgg tcgctacgcg agtccgtacg agatcgcagc tgccgtcacg tggctgggtt
      661 cggagagcgc cggatacatc gccggagccg tcatccccgt cgacggcggc ctcggcatgg
      721 ggcactaggc atccgctccc cgaacacgga aaggctccca tgggactgct cgagggcaag
      781 cgcctgctca tcaccggtgt catcacggat gcgtcgctgg cgttccacgc cgcgaagatc
      841 gcccaggaac agggcgccga cgtggtgctc accggtttcg gacggatgcg gttggtggag
      901 cggatcgcgc agcggctgcc gaagcctgcg ccggtcgtcg agctcgacgt ctcggaccag
      961 tcccagctcg acagcctcgt cgagcgggtg tcgccacacc tcgccggtgc gaacggggag
     1021 gaggagccga agctcgacgg cgttctccac tcgatcggat tcgcgccggc gtcctgcctg
     1081 ggggagaacg cgttcctgaa cgcgccgtgg gaggacgtcg ccacgacgat ccaggtgagc
     1141 gcctactcgt tcaagtcgct gtccaccgcg gtgctgccct tgctcgggcc gggcagctcg
     1201 atcgtcggca tggacttcga caaccggcag gcgtggcccg cctacgactg gatgggcgtc
     1261 gccaagtcca ccctcgagtc ggtcacccgg tacctggcgc gcgagctcgg gccgaagggc
     1321 atccgggtga acctcgtcgc ggccggcccg gtgcggacga tggccgcgaa gtccatcccc
     1381 ggcttcgccg cgttcgagga cgcctgggac gggcgcgcgc cgctcggctg ggacgtcaac
     1441 gacccggtgc ccgtcgcgaa gaccgtctgc gcgctgttct cggactggct gccggtcacc
     1501 accggctcga tggtgtgggc ggacggcgga ttccacgcca tcggtgtcta atcgagttgt
     1561 gcgaaaacag gttgacgccc tgctggtgct gagcttcggc ggccccgaag ggcccgagga
     1621 tgtccggccc ttcctggaga acgtcgtccg cggccgcggc atcccccctg agcggctcga
     1681 cgccgtcgag gagcactacc gacatttcgg gggcgtgagc ccactcaacg cgcgcaaccg
     1741 cgacctgatc gccgcgatcc ggcagcgcac cgacctgccc gtgtacttcg gcaaccgcaa
     1801 ctggcatccg atggtcgagg acaccgtcgc cgagatgatg cgcgacggcg tccgccgcgc
     1861 actcgtgttc gccaccagcg cgtacggcgg gtactcggcg tgccggcagt accaggagga
     1921 catcgtgcgg gcgcgcaaga ccgtggggga tggcgccccg gaactggtga agctgcggca
     1981 cttcttcgac catcccgagt tcgtcgccgc caacgccgac gccgtgcgcg ccgcgggtgc
     2041 gagcctgccc gccgacctcc gcgacgacgc tcggctcgtg ttcaccgccc actcggtgcc
     2101 ggtcgccgtc gacgccgctg gcgggccgcc cgaggagggc gggcaccggt actcgcgaca
     2161 gatagccgag gccgcacggc tggtcgcggc ggagctgggt gtcgcagagt acgacctggt
     2221 gtggcagtcg cgctcggggc cgccggaggt cccgtggctc gaaccggaca tcgtcgacca
     2281 catcgacgcg ctgcgcgacc gtggcgtggc cgcagtcgtg gtcgccccgg tcgggttcgt
     2341 gtccgaccac gtcgaggtgg tgtgggacct ggacacggag gcgcgggagc gcgccaccga
     2401 gctgggcatg ggcttcgcgc gcgcggcgac ggccgggccc gacccgcggt tcgccgacat
     2461 ggtggtggag ctggtggccg agcacgtcca cgacgccgcc ccgcggtccc tgggtgccgt
     2521 gccgagtgcc ggctgcacgg tcaacggtgc cccatgtgcc gtcgactgct gcgccccggc
     2581 tcagcgcccg tcgcggccgg agtgagcgag cctcgcgcga acggcactct cgtccggtaa
     2641 tgcgggacga accggccgtt cacgcgatgg accctccgct cgtgcctgcg gtgcaaccgg
     2701 gccgaagccg cacaaacggc gctctcgttc accagtgccg gacggacggg ccgtgtgtgc
     2761 gagaacctca ggcctgctcg ggcaccccgg tgtagtagaa ggctgccgcg cggtcggccg
     2821 cggctcgggc cgctgcgggg tcggtgccgg ccgaggtatt ctccgcgccc caggcgtcac
     2881 tggccgacgt caggaactgc ttgcttgccg tcatccgtgg cgaaccctgc ctcgctttcc
     2941 gcctggtcca ccgcccggcc ccacgacacg tgcatggcca gccccacgag gacgatgtcc
     3001 cagccgatgc cgaccgcacc gggaccgtat tcgttccagc gttcgtcgtc gacatgggcg
     3061 atgtgctcga tctcgaaccg ggtgccggct gtctgacacc tcggtcagcg ggacctcgat
     3121 ccaggccacc tcctcgccgc tcccaggtgc ggcgaaaccg tgcggcggct cccgcgagtt
     3181 cgctcccacc catcagccaa atgtccttgc cctcctcctg ctacagcgtc cgcaccgtcg
     3241 ctgtcgggtc ggtggcgacg tcgttgcaga actcgattcc aacatcgggt tccacctcgg
     3301 cgcgggcctg cccggtgacc gcacgggagt ggtcctgctc tacctggcga tgctcggact
     3361 gatcgtccac cgtcccggac cttctcgcac cttacgggct cgacgccctg atcgacgcga
     3421 tggtgacgcg cctcctcccg gcgctgaccg ccgtccgcgc agagtcggtg tgctccgcgc
     3481 agtgcccccg gagcctgcga cgagcagacc gggtctgcga ggacgactac ggcatggggt
     3541 ctggggcggc tgcgattgcg gccgctacca ggtctgccgc cgtgctcgct cgtgtgcgcc
     3601 tgccccggtc gtagtgctgg gtggtggcga tcgtggcgtg cccgacgtcg gcctggacgt
     3661 gctggatgcg ggcgtcctgc tccaacgcga tcgtgacgta ggcgtgccgc aacgcgtgcg
     3721 ggtgcacccg gtcggcgagg ccgtccagga ccggcccggc gtgggccgcg atccggcgta
     3781 gcagcgtgta gaggtccacg cgcgagcagc ggccgccgtt gcgggtggcg agcaggggcg
     3841 tcccacctgc gccggtgcgg ccccgccgcg cgggcagcgc cgcgtcgccc acgcggtcgc
     3901 gctcggcgag gtaggccgtc agcgcgtcac ggacgaggtc ggtgaggtag acctcgcggt
     3961 gcagtccacc cttgccgagc acgcggagta cgtcgtcgcc gcccgtgcgg tagaggtcgc
     4021 ctcggtcgag gcccaccacc tcggagatcc gcaggcccag cgtcagcagc gccaccatgg
     4081 ctaccgcgcg cagggcgtac aaccgggcca gccgggggct gcgggagtag ggcagccggg
     4141 ccgccgcgac gagcagcgcc ttgagctgct cggccgtgag ccgcacggtc ggggacgcat
     4201 ccctggcggt cgacgacagc cccagccgcg cccggttgag cgccgccgga ttcgccgcga
     4261 cgacgccggt ctcggccagg tgtccgtaga gcgccgacag cgtggagagc atccgctggc
     4321 gggtgcgtcg ctgcgcgccg gcggcgtcaa gggcgtgcag ccaggccttg acgtggcggg
     4381 ccgtggcgtc gcgggggtcg atggcctgcc gcgcgcacca gcggaaccag gcgaggtcgt
     4441 gcagcgggcc ggcctgcgcg ggaggcgggg cggcttgtcc ggaccccgcg ggcggcgctc
     4501 ccccgatggc gtccacccag gcgatcggca gacccagggc gtaggcgtac gtgcgacggg
     4561 tgccggcgtt ggcgtactgc ccgagccagt cgacgagctg ggcgcgcagc ccctcgggat
     4621 cgtcgacgcc gggccagtcc gagaccccag gggtgacggg caccggcgcg gtaggggagc
     4681 gatgacccgc gagtgcttcc aggatggcca acggctcgga cgctgccacc agcggagcct
     4741 agcggtgtgt tcctcctgct cggcctctcc gggtgcggcg tgccggccgg tgaccggttc
     4801 ggtccgcaaa ggctcatccg gccgccgtcg gtgcgccgga agccgttacc ggcgcgctcg
     4861 ctgccagggg tctcacctgc gggcagaatt gagcgcatgc gagccgtgat gggaggttcg
     4921 atgaatccgc gttcgtccgg cgagcctgtc gcgagctcgg gcgccctcgg cgtcggcccc
     4981 gactcgcgcg gatcgcggtc ggcgcccccg cccctacgga ggcaacggcc tgattggcgc
     5041 ccttgccgcc ccgcaccgcg tgcgcggaga ccccggcatc gtctccccgg cgcgggaaag
     5101 ccgaccgcga cgacgacgtg gtcgatgttg accgagccga tgacagcacg gacccgtcgt
     5161 cacgcaacgg cgtcgcggcc ggcctcgtcc ccggcgatgg caccggtgat gagaccgacc
     5221 agctcgtctc ccgtggtgtc ggggatgctc cggcgggccg cggtccggcc gcggcgcagt
     5281 acgtaggcgg tgtcggcgac ccgcatcacc tgccccatgt cgtggctgac cagcagcacg
     5341 gtgacgcccg actcccgcag tctccggatc agctcctcca cggccgccgt ctcccgcacg
     5401 cccagcgcgg cggtcggctc gtccatgatc accagcgtct cgccccagtt ggcggccctg
     5461 gcgatggcga tgctctggcg ctgcccgccc gacatccggc ggacggtggc gcgcacattc
     5521 ggcacgtcga cgtccagccg cgagaggatc tccttgctgg acgcgatcat cgccttcctg
     5581 tccaccaccc ggaacgggcc gaaccccgtg gtcagctccc ggttgaggta gaggttctgc
     5641 cagacctgca ggtcctcgat cagcccgagg gtttggtaca cggtctcgat accgagcctg
     5701 cgcgcgtcgt ccggcgaggt gagccggatc ggctcgcccc ggaaccggat ctccccccgg
     5761 tcgggtgggt gcaccccggt gaggcagcgc accagggtgg acttcccggc cccgttgtcg
     5821 ccgaggagag cagtgacctc ggccttgtcg atgctgaggt ccacgccctg cagcgcgcgt
     5881 accgcgtcga aggacttcgt gacgccggtg atctggacga gcggtgagcc gctgtcggtc
     5941 atggcgaccc tcctacttct ggaaccggga gatgaacgcg gcaccgagca ccaccacgcc
     6001 caccgcgatg ggttggtagt actgcgagat gccgatcagc gtgaacccgt tgatcaggct
     6061 ctgcagcagc accgcccccg ccgccgagcc cagtaccgag gcgcgaccgc cgaacaggct
     6121 cgacccgccg agaaccaccg cggcgatgga actgagcagc aggtcggtct gcaggctcgg
     6181 gtccgcaccg ccggtgcggg ccacgagcag cacgctggcc agtccgcagg ccagcccgga
     6241 cccggcgtac gcgagcagcc ggatgcgcga cacgttgata cctgactcgc gtgcggcctc
     6301 gatgttgccg ccggtggcga ggaagtaggt gccggtccgg gtgcggaaca acaggaagcc
     6361 gaccacgagc gccaccagcg cagcgaccac ccagaaccag cgcagcgggc cgatcccgtc
     6421 cagcgccagc ccctgcagga agctcgagtt gaccgacacc gtgttgccgc cggtcagcag
     6481 cagcgtgatt ccgctgacca cggacagcga accgagcgtg acgatgaaat ccgtcatgct
     6541 cagcttggcg accagcaccc cgttcaccac gccgaccagc atgccggcga gcaccgcagc
     6601 caggatcgag acggccaggc cccagccgtg cgagtaggcg aacccgagca ccgcggccgc
     6661 ccacggcagg ttggagccga ccgagaggtc gatgccggcc acggtcacca cgagctgctg
     6721 gcccatcgcg agcaccagca ggatcgccgc cgacaccagc aggtcgctga tgttgccgac
     6781 cgtcaggaag tccggcgtag cgatgaagaa cgcgacgaac acgacgacca ggccgatcgg
     6841 ggcggcgtag cgtgcggcgt cgcccatcga gaagcgacgc cgctcgttcc gtggcggaac
     6901 ggcgaggtca gtcatgggtg gtcacttccc tccggccacg gggttgtcga atggcgcgat
     6961 cggctgcggg aacgaggcga tcgccttgtc cgcgttgctg gcgtcgatga gctggatcgg
     7021 cgccacgatg cgctcgggca cggtctgctt ctgcaccagc ttcatgcagg cctgcaccgc
     7081 catctggccc tcggcgtagg gatactgcga gatcgtgccg gtcagcgtgc cgtccttgac
     7141 ggcctgcagc gcggctgtga tgccgtccac ggagatgatc gagaccttgc ccgtgagccc
     7201 cgcgttacgc accgactgcg cggctccgag gcccatggtg tcgttcgccg cgaagatgcc
     7261 ggtgatgtcg gggtgcgcct tgaggatcgc gtcggtcacc gtctgcgcct tggcctggtc
     7321 gtagtcggcg ggctgggttg ccaccacctg gagcgcaccc gcggtggcct tctcgaagcc
     7381 ctgctggcgg ttgatgccgt tctgctcgcc ggcgatgccc tgcaggatcg ccaccttgcc
     7441 ggtgcctccc atcttggcca tcagggcctt cccggcctgc tcgccggcgg acaggttgtc
     7501 ggagccgatg aacgaggcgt aggagacacc ggcctgcgcc gacgcgttcg ggtcgatttg
     7561 cgtgtccagg ttcaggatcg ggatcttctt gcgcgccacc gggatcagcg gggtgatcac
     7621 gttggtcgcg ttgaccggga ccaccccgaa acagctgacg ttctgcccgg cgagcgtgga
     7681 gatctgggcg ttctccccgg tggagtcggt ctcgctgctc ccggcctgca cgtcgacggt
     7741 gacgccctgc gccttgccct cggcgaccgc gccgtcccgg agcgccgccc agtagggatt
     7801 ggtgaagtcg cgggtgacga tggcgatccg tacgttgccg gatccgccgc ccgatgcggc
     7861 ctgctcgccc cgcccgcacg ccacgagacc cgtacagatc gtggcggccg cacagaggac
     7921 gagcgcggtg cgaagtggtg tcattgcggt gttcatggtg gttcccttct ccagctccgg
     7981 ggattcgttc gatcagctgt gtgcgtccgg tggcggcccg caggagctcc gccgcaccaa
     8041 ctggcaggtc agggccgtgg tcggtggcgt ctctcccgcc aacagggcgt acatcgaccg
     8101 catggcggcc tcggcgagct cgaccaccgg ctgggccacg gtggtgagtg gtgggtcgag
     8161 caggtcgaac cacggcgcgt cgtcgtagcc gacgagcgac acgtcacggg ggattcgcag
     8221 gccggcgcga cgcagcgctt tcatcgcgcc gatggccatc aagccgtcgg cggcgacata
     8281 cgcggtgggc gggtttggtg aagcgagcag gcgctccacc gcgagctcac cgctgtgctg
     8341 ccggaaatca ccgctcacca cctcgacggc ggacgcggcg agcccctgcc cggtgagcgc
     8401 cccgagcatc atgtcgtggc gctcccggcc gatctgggtg gtctggggca gcgcgatgat
     8461 cccgatccgg cggtggccga ggccgatcag cagctcggcg acctcgccca gagccgcagc
     8521 acccgagccg cgcacgaccg ggatggcgcg cagccactcg gacacaccgc cgaccccgtc
     8581 cgccgcgatg ggccggtcca gcagcaccat cctggcccgt gccgcacccg ccgcgaggat
     8641 ccccggggtc atcccgccgg ccggcacgag catcacgccg tccacctcac tcccggcgag
     8701 cagccgggac aggtagccgt cctggcgtgc ggcgtcctca tcggagttgc acagcacgac
     8761 cgagtagccc tgggcggcgg cgatgcgctc gatccgccct gcgagctcgg cgaagaacgg
     8821 gttggccacg tcgctgacca ccaggccgag cgcccgggtg gagtcggtgc gcagcgagcg
     8881 ggcggcggca ttcgggcggt agccgagctc gtcggcgatc gcgatgacgc gtctacgcgt
     8941 ggcctcggat acgcggctgt tcccggacag cgcgagcgag acggtggcct cggagacgcc
     9001 ggccaaccgg gcgatgtcgc gcatccgaac agccatggca ccgcacttcc tgtcgacgtc
     9061 gacacgaatt caatcgatta actgggccga gaatcgcaca ggtgcgggcg aaggggaagg
     9121 tccggtctgc ccccgcccct cgagcagcga accagagtgg cgttggttgg ccgtcgatcc
     9181 ctgctctggt ggagcagcga gacccaccgg ctgacaggag acgaaggacg gcctacgtcg
     9241 accgcacatc agcgacatac ccggtgcagc tctcttcgct gaacaactcc gggcaccttc
     9301 ggcgatcgcc atcaccaggg cctgtccggt cagttttccc tgtcgaacca ccgaactgat
     9361 caagatggtt gagcggcgat caagatcatc gatctgcgac gactgtcctg gttcagccat
     9421 gccgatgcgg agatacgtcg ctgaccagcg cgcattccgt tgtgttcggc cgtgctgttg
     9481 tggctcggat ggtgccgcga gcgtggtcgc gatgggccgt cggtccctgc gtgggcgaag
     9541 cggccggcgg tgccggccgc cgagagccgg cccgtcgatc gacggcccac cgccggggcc
     9601 aggcacgcga ggacttcgga ctggagaccg tctactggga cgccggcacc ggccggctct
     9661 gccccgcctg ctcaaggggg ccgtacgcgg gggccggtgt tcgttaccca ccgccgaccc
     9721 ggaccggcga gggtcgtcag cccgcgcgac gtgtgcccgg acacggggct gtcggaccaa
     9781 cctagccaga acggctgcgc cccgaacacc accttcgccg cgcccaggac agcgacgacc
     9841 ccctcttttt ttgccccgcc accgccatac gaaccaacga aacagtggtt tcgttggtag
     9901 gtagtttggt tttcgaaact cacaaccgct tgcacagttg tgtaactcga actgctaggg
     9961 tgctgatcat gatcgacgag gccctacgct gcggctacag caagtgccgg gccgagctgc
    10021 ccccgcccgg cccccagggc ggcaggcgcc gcgccttctg ccgggacacc cgttggccgg
    10081 gtggccggac gtgcgcccag atggcccggg ccgagcgcga cgcactcgac gcgctcggcc
    10141 tggactcggg ccgcgcgacc ttccggctcg acgccgaccg gctgcgggag cacgtcgagg
    10201 ccgtgcgtga accggttgct gagctggccg gggcgctttc ggccgtctcg gcccggctcg
    10261 acgaggtgga gggctccgct ctcgccgcgg tcgaggcggc caaccgtggg gccgccgagg
    10321 ccgaggccgc gcgcgtcgac gcccagcaag cccgggagcg ggcggagcgg gagtcccggg
    10381 aggcgcgcga ccgggcggcg cgggcggggg aggagcgtgc ggccgccgtc gaccgcgcgg
    10441 ctgccgcggc acgacaggcg ctggaggcga ccgaggccct gggtgcggcg cgtcaggtgg
    10501 cggaggaggc cacggcggcg cggcaggccg ccgaggacag ggccgttgca gccgcccggc
    10561 aggtcggcga ggccgagcgg gcgacgcagg aggcggccgt ccgggccggg cgagccgacg
    10621 ccgaacgagc cgccgcgcag gcccgcgcgc aggagtggca ggccgagtcc gaggaacggc
    10681 gggtcgagct cgacgccgtc aggtcgcagc tggcggccgt cgcgaacgcc cgcgacgaca
    10741 ccgcgcgacg ggcctcggcc gccgctgccg ccctggccga ggagcgagag gggcggcagc
    10801 gggccgatca ggagctggcc ggtgcgcggg agcgcgctgg ggtggagatg gccctgcgga
    10861 cgcgggcgga cggcgagctc gagcggctgc ggacggaggt cgagcgagcc cacgccgacg
    10921 cccggctggc gagggccgaa ctgactgccc tgcgggagcg cggcaccgac gaactgcggg
    10981 ccctcgtcac ggccgccgtg caggggaagg ccctctgacc ggagatgcag ccgttggcgg
    11041 atgcaccatg gtcgtatgac ctccccgctc cgctggttca tcaccggcgc atccaccggc
    11101 ttcggacgca ccttcgcgga ggtgctgctg gcccagggcg accacgtcgt cgcgaccgcc
    11161 cgggacaagg gcgccgttgc cgacctggtg gagcgctatc ccgacgcggc gctcgcgctg
    11221 ccactcgacg tcaccgaccc cgagcaggtc cgtgaggcgg tggccgccgc caccgaggcg
    11281 ggccccgtcg atgttctggt caacaacgcg ggccacggcc tgctcggcgc actggaggag
    11341 ctgtccgacg agcagatcca gcaggtgctc ggcgtcaacc tgctcggagc gctggccgtc
    11401 acccgggccg tgctgccgca catgcgcgcc cggcggagcg ggcacatcgt gcagatgtcg
    11461 tcggtcggcg gcgtcgtcgc caacccgggc cacgcggtct acgcgaccac gaagttcgcg
    11521 ttggaggggg catcggaggc gctcgcgggc gaggtcgcac cgttcgggat ccacgtgctg
    11581 atcgtcgagc ccgggccgtt ccgcaccgac ttcctgggcc ggtcgctcga aatggccacg
    11641 ccgatcgcgg actaccaggg cggaccggca ggggcgctgc gcgcccggtt cgcgacgcag
    11701 cacgggctgc aaccgggcga ccccgttctg gcgatcgaga cgatcatcgg ggtggttcgc
    11761 gccggctcgg cgccgttgcg cttgccgctg gggcacgcgg ctgtgcagcg catccgagag
    11821 aagctgcagc gccagctgga cgacctcgag gcgtacgcgg acctgtcgat gtcgaccgac
    11881 cgtccggtgg gctgaggcgc gtccgtcggt cggcgcgttc acgcaatggc ggtgcgacgg
    11941 cgaaaacatt cggacgatgt gaggattgtt catgcgaatc ggcttgggct taccccatta
    12001 cggcgcgctc gccaccgtcg acgctctgct gcaggtcagc cgggccgccg aggcgatggg
    12061 gtacgacagc ctgtggacgg gcgatcgcct tttcgtcccg gtccgtccga gcaaccccta
    12121 cccggacgcc agtggcgtca ttccgcccca gttcgcgaca tacatggatc cgctcaccgc
    12181 attgacgttc gcggccgcgc acaccgagcg ggtccggctg gggatgagca cgctcaacgt
    12241 tctgtggtat ccgccggtgg tgctcgcccg gaccctcacc acgctcgaca tcctcagccg
    12301 cgggcgggtc gacgcgggat tcggaatcgg ctggatgcgc gacgagtaca cggcagtgaa
    12361 cgtcccgtgg gagcgccggg gcgcgcgcct cgacgagacg ctcgacctgc tggagaagat
    12421 ctggacctcg gagctcgtgc accacgacgg cccgttgttc acggtcgtcc cgacaccgat
    12481 cgaacccaag cccgtgcagc gcccccggcc gccgatcctg ctccccggtt tcacaccgca
    12541 ggccatgcgg cgggtcgggc ggcgggccga cggatggctc gcagtcggtg ctcccctgtc
    12601 cggcctcggc tacctctgga gcaccatccg acgggaagcc gaggacgccg gacgcgaccc
    12661 cgccgagatc cggttcatcg tccggatgaa tccccacctg acggacgtga aggccgacac
    12721 cgagaaggtt ccctcgaagg gcaccatgga ccaggtcatc gactatgccc gatgcctggt
    12781 ggaggtgggt gcggacga
//
LOCUS       DASRSC010000219         4935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2700611,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000219 DASRSC010000000
VERSION     DASRSC010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2700611"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VFE39_10715"
     CDS             complement(<1..499)
                     /locus_tag="VFE39_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HET6259112.1"
                     /translation="MTARTTFPSVAPSIAVVGGGVSGLAAAHRLRVLLGPSATISVLE
                     QRDRTGGVLHTVDLAGVPFDVGAEAFLARRPEMAVLLGELGLTGEQVHPTAAKASLRV
                     NGRTAALPGATLLGVPTSDARLDGLLSATGLAAVAAEPALPLHWQRGSDVALGGLLRH
                     RFGDEL"
     gene            complement(496..1560)
                     /gene="hemE"
                     /locus_tag="VFE39_10720"
     CDS             complement(496..1560)
                     /gene="hemE"
                     /locus_tag="VFE39_10720"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HET6259113.1"
                     /translation="MVLNPPSDVVARRDLRTAPLLAAARGESPEHIPVWFMRQAGRSL
                     PEYRALRAGSGMLQACLTPDLTCEITLQPVRRHGVDAAILFSDIVIPLYIAGVGVDIV
                     PGTGPVVAEPVRTMADVDRIPALHPDQVAPVAEAITLLLRELGETPLIGFAGAPFTLA
                     SYLVEGGPSRNHERTKALMRSAPEVWHALMGRLAESTSVFLRAQIDAGVDAVQLFDSW
                     VGALSDRDYRAYAQPHSARVLTGLADTGVPRIHFGVGTGELLGAMAEAGADVVGVDWR
                     IPLDEAARRTGGTHPVQGNLDPAVLFADQPSIEAEARRIVDEGRRTPGHVFNLGHGVL
                     PATDPDVLTRLVELVHSLRP"
     gene            1679..2260
                     /locus_tag="VFE39_10725"
     CDS             1679..2260
                     /locus_tag="VFE39_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3000 domain-containing protein"
                     /protein_id="HET6259114.1"
                     /translation="MPDATAPPPEFRQAVASLRAMRPRPELIVSALDAPPRLAPYTWA
                     FSVEADASTAPGEELDEPDTSGRLILLHDPAGQDAWEGTFRLVCFVQARLDPEQLGDE
                     MLPVVGWSWLTEALDHGGAEYVALGGTVTQTSSVRFGDIAGPRRDDDVELRASWTPTG
                     PDLSRHAEAFCALIASAAGLPPVGILPLAHRTV"
     gene            2564..3217
                     /locus_tag="VFE39_10730"
     CDS             2564..3217
                     /locus_tag="VFE39_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017976119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6259115.1"
                     /translation="MVPHPREELFSVLVVDDHPLLREAIAARLRSMGAGTVYEAASVA
                     EARARAHANGPCDLAILDLGLPDGSGLDLVTELRSLGWNRLVVLASSDDPYAVRSAFQ
                     AGAQAYLLKSASAAIVTDGVKRVLDGGVYADPTVAPLLATGTRVPGTDNTPRELSARE
                     VEVLQLVADGQSNKEIGEALSLSALTVKSHLSRIGRKLGTGDRAQMVALAMRAGVIR"
     gene            3280..4458
                     /locus_tag="VFE39_10735"
     CDS             3280..4458
                     /locus_tag="VFE39_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HET6259116.1"
                     /translation="MLAPRDGLPSVVADRRALAHTAAAFAAGSGPIAVDAERASGFRY
                     SQRAYLVQLRRAGAGTALVDPIPLQSDLSELAPALTGPEWVLHAANQDLACLAEVGLV
                     PSALFDTELAGRLAGLPRVGLGPLVEQLLGLSLEKGHGAADWSRRPLPEDWLVYAALD
                     VEVLVELRDVLAGLLAEQGKLEWAIQEFEAVRTAGPPRPRAEPWRRTSGLHKLRKPRA
                     LAAVRALWEARDRLAAERDIAPGRVLPDAAIVEAAVAAPEAARDLAALPVFRGRSQRR
                     LTAYWYAALAEAAGLDAAELPKIAPPAEGPPPVARWADREPDAAARLAAARTAIAAVG
                     EERSVPVENLLQPDLLRRLCWSPPADGDVAGALRTGGARPWQIDLLAPLLEPALQARG
                     "
     gene            complement(4459..>4935)
                     /locus_tag="VFE39_10740"
     CDS             complement(4459..>4935)
                     /locus_tag="VFE39_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6259117.1"
                     /translation="TEGAGLADVRPVDLAVLVVPALAAVRADAARRGLRVQVRVAPAP
                     ATGDPVLLERVAGNLLENAVRHNVVGGWMDVCTGVRDGYAELQVSSAGPAIPADRVQE
                     LFEPFRRGPVARTGNAPGSGLGLSIVRAVVQAHGGVVHAVPVSGGGLAVTVRLPAA"
BASE COUNT          682 a         1824 c         1773 g          656 t
ORIGIN      
        1 cgagctcgtc accgaaccgg tggcgcagca gcccgcccag cgcgacgtcg gaccctcgct
       61 gccaatgcag cggcagggcg ggctccgcgg cgacggcggc cagcccggtg gcggagagga
      121 gcccgtccag tctggcgtcg ctcgtcggca cgccgagcag ggtcgcgccg ggcagggccg
      181 ccgtacgccc gttcacccgc aacgacgcct tcgccgccgt ggggtgcacc tgctccccgg
      241 tgaggccgag ctcgcccagc agcacggcca tctccggccg ccgggcaagg aacgcctccg
      301 cgcccacgtc gaagggcact ccggcgagat cgacggtgtg cagcacgccg ccggtgcggt
      361 cgcgctgttc gagcacgctg atggtggccg acgggccgag caggacgcgc agccggtgtg
      421 ccgcggcgag cccggagact ccgccaccga cgacggcgat gctcggggcc acgctgggaa
      481 aggtcgtccg cgccgtcacg gccgcaggct gtgcaccagc tcgacgagcc gggtgaggac
      541 gtccggatcg gtcgcgggga gcacaccgtg gccgaggttg aacacgtgcc ccggtgtgcg
      601 gcggccctcg tcgacgatcc ggcgggcctc ggcctcgatc gacggctggt cggcgaagag
      661 cacggcgggg tcgaggttgc cctgcaccgg atgagtgccg ccggtgcggc gggccgcctc
      721 gtcgagcggg atccgccagt cgacgcccac gacgtccgct ccggcctcgg ccatggcacc
      781 gagcagctcg cccgtgccga cgccgaagtg gatccgcggc acgccggtgt cggcgagccc
      841 ggtgagcacc cgggcggagt ggggctgagc gtaggcccgg tagtcgcggt ccgacagcgc
      901 accaacccac gagtcgaaga gctggaccgc gtcgacgccg gcgtcgatct gggcccgcag
      961 gaacaccgag gtcgactcgg cgaggcggcc catcagtgcg tgccagacct cgggggccga
     1021 cctcatgagg gccttcgtgc gctcgtggtt gcggctcggg ccaccctcga cgaggtacga
     1081 ggcgagggtg aacggggcgc ccgcgaagcc gatgagcggg gtctccccca gctcacgcag
     1141 cagcagcgtg atcgcttcgg ccaccggagc cacctggtcg gggtgcagcg ccgggatccg
     1201 gtcgacatcg gccatggttc gcaccggctc tgccaccacc gggccggtgc ccggcacgat
     1261 gtccaccccc actccggcga tgtagagcgg tatgacgatg tcgctgaaca ggatcgccgc
     1321 gtcgacaccg tggcggcgca ccggctgcag ggtgatctca caggtcaggt cgggcgtcag
     1381 acaggcctgc agcatccccg acccggcgcg cagcgcccgg tactccggaa gcgagcgccc
     1441 cgcctgacgc atgaaccaga ccgggatgtg ctccggggac tcgccgcgcg ccgcggcgag
     1501 caggggcgca gtgcgtagat cgcggcgggc gacgacgtct gaagggggat tgagcaccat
     1561 gagaagtaca ttctctcacg gctcggcgtt ggatcacccg acggtggacg gcgcgggccg
     1621 tgaatcatgc ggcgcgcctc ggcacccgga cacggagagc gacctagagt tctcgctcgt
     1681 gcccgatgcg accgccccac ctccagagtt ccggcaggcg gtcgcctcgc tgcgcgccat
     1741 gcgtccgaga ccggagttga tcgtctccgc cctggatgcg ccgccccggc tcgcccccta
     1801 cacgtgggcg ttcagtgtgg aagcggacgc gagcacggcc cccggcgagg agctcgacga
     1861 gccggacacc tccggccggc tgatcctcct gcacgacccg gccgggcagg atgcctggga
     1921 gggcacgttc cgcctggtgt gcttcgtgca ggcccggctc gaccccgagc agctcggtga
     1981 cgagatgctg cccgtcgtcg gctggtcgtg gctcacggag gcactcgacc atggcggagc
     2041 ggagtacgtc gcactcggcg gcaccgtcac gcagacgtcc tcggtgcggt tcggagacat
     2101 cgcaggcccg cgccgggacg acgacgtgga gctacgcgcc tcctggacgc ctaccggacc
     2161 cgacctgtcc cgtcacgcag aggcattctg cgcgctcatc gcatcggcgg cgggccttcc
     2221 cccggttggg atacttcccc tagcgcatcg gacggtctga cctggcgttc acctgggtct
     2281 gagactccga agagatcagg tccgtcaccc gtacgggtgg atgcatatcg acaacgctgt
     2341 aactttcacc cggagaactt cgaaccacca cacgacgtta gaccgcttgg ggggctaggc
     2401 ttcctccacg acacctcctc gggcggtcgg gcgacagctt catcgaccgg ggttctggtg
     2461 ccggtcgggg ggcaacgacc gactccaggg aggtagtgac gtggccgtag tgggccaggg
     2521 cgcaccagct cgcggcactc cgggtgccgt gctgtcgccc tcgatggtcc cgcatccgcg
     2581 ggaagagttg ttctcggtgc tggtggtcga cgaccacccg ctccttcgcg aggcgatcgc
     2641 cgccaggctg cgctccatgg gcgcgggaac ggtgtacgaa gccgcctcgg tcgccgaagc
     2701 acgggctcgg gcgcatgcga acgggccctg tgacctggcg atcctggatc tcggtctgcc
     2761 cgatggcagc ggtctcgacc tcgtgaccga actgcggtcg ctcggatgga accggctcgt
     2821 tgtgctggcg tcctcggacg acccgtacgc cgtgcgctcg gcattccagg cgggtgcgca
     2881 ggcctacctg ctgaagtccg cgtcggcggc gatcgtcacc gacggggtca agcgcgtgct
     2941 ggacggcggt gtctacgccg atccgacggt ggccccgctg ctcgccacgg gcacccgggt
     3001 gccgggcacc gacaacacgc cccgcgagct ctccgcccgc gaggtcgagg tactgcagct
     3061 ggtggccgac gggcagtcca acaaggagat cggcgaggcg ctgagcctgt ccgcgctcac
     3121 ggtcaagagc cacctgtccc ggatcgggcg caagctcggc acgggggacc gcgcccagat
     3181 ggtcgcgctg gcgatgcgtg ccggggtcat ccgctgaccg cagatccgca tcaggacaag
     3241 gggcagtacg acccgccgga cgctccagca gcagttccgc tgctggcgcc ccgcgacggg
     3301 ctgccttcgg tcgtcgccga ccgcagggcg ctcgcacaca cggccgcggc cttcgcggcg
     3361 gggagcggac cgatcgccgt cgacgccgag cgcgcctccg gcttccggta ctcgcaacgg
     3421 gcctacctgg tacaactgcg ccgtgcgggc gcgggtacgg ccctcgtcga cccgatcccg
     3481 ctccagagtg acctttccga gctcgccccg gcgctgaccg ggccggagtg ggtgctacac
     3541 gcggccaacc aggacctggc gtgcctcgcc gaggtgggtc tcgtcccctc cgcgctcttc
     3601 gacaccgagc tggccggacg gctggccgga ctcccccgcg tcggcctcgg ccccctggtc
     3661 gagcagctgt tgggtctctc cctggagaag ggccacggtg ccgccgactg gtcgcggcgc
     3721 ccactgcccg aggactggct cgtctacgcc gcactggacg tcgaggtgct ggtcgagctg
     3781 cgtgacgtac tcgccgggct gctcgccgag cagggcaagc tcgagtgggc gatacaggag
     3841 ttcgaggccg tgcgcaccgc cgggccaccg cggccccgcg ccgaaccatg gcgacgcacc
     3901 tccgggctcc acaagctgcg caagccgcgc gccctggccg ccgtccgggc gctgtgggaa
     3961 gcgcgtgacc ggctcgccgc cgagcgcgac atcgcaccgg gacgtgtcct ccctgacgcc
     4021 gcaatcgtcg aggctgccgt cgccgcgccc gaggcggcgc gtgacctggc agcattgccg
     4081 gtgttccgcg gccggtccca gcggcgcctc accgcgtact ggtacgccgc actggccgag
     4141 gccgccggcc tcgatgctgc ggagctcccc aagatcgctc cgcccgccga ggggccgccc
     4201 ccggtggcgc ggtgggccga ccgcgagccc gatgcagccg cccgcctcgc cgcggcccgt
     4261 accgcgatcg ccgctgtggg cgaggagcga tcggtgccgg tggagaacct gctgcagccc
     4321 gatctcctcc gtcgcctgtg ctggtccccg cccgccgacg gcgacgtggc aggcgcactg
     4381 cgcaccggtg gggcgcgccc ctggcagatc gacctgctgg cccccctcct ggagcctgca
     4441 cttcaggccc ggggctgatc aggccgctgg caggcgcacc gtcaccgcca gtccgccgcc
     4501 cgacacaggc acggcgtgaa cgactccgcc atgggcctgg accaccgccc gcacgatcga
     4561 gagccccagc ccggaaccag gcgcgttgcc cgtgcgcgcc accgggccgc gacggaacgg
     4621 ctcgaacaac tcctgcaccc ggtcggccgg gatcgccggg ccggcggagg acacctggag
     4681 ctccgcgtag ccgtcccgca cgccggtgca gacgtccatc cagccgccga cgacgttgtg
     4741 ccgcaccgcg ttctccagca ggttccccgc cacgcgctcg agcagcaccg ggtcgcccgt
     4801 tgccggtgcg ggcgccaccc ggacctgtac ccgcagccct cgccgcgcgg catcggcgcg
     4861 gacggcggcc agtgccggca cgacgagcac ggcgaggtcc acgggccgca cgtcggccaa
     4921 cccggcacct tcggt
//
LOCUS       DASRSC010000220        15198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3521214,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000220 DASRSC010000000
VERSION     DASRSC010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 15198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15198
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3521214"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1943)
                     /locus_tag="VFE39_10745"
     CDS             complement(<1..1943)
                     /locus_tag="VFE39_10745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HET6259118.1"
                     /translation="MVLGLVAAVVLAVMSLPVAGGAGALAAQVSSGSVNVAPSALLAG
                     NTPNVTTVTDSTGKPIAYLYDQFRVPATSAQISPAMKAAIVAIEDKRFFEHGAIDPVG
                     TLRALVNDAGGGAQQGGSTLTQQYVKNFELYVAATTDAQRQAAVAPSLARKIHEAELA
                     VQLDQELSKDQILTRYLNIVYFGHGAYGVAAAAQVYFGTTAAALTIPQAALLAGMVQS
                     PTQFDPIQHPDAAKSRRDLVISQLRQVGSITDADAAAATAAPLGVQSAAAVPAEGCTA
                     AGDNGYFCSYVVRYLEQAGFSSQQLSSGGYTIRTTLDPQAQATAKAAVNSQVPPTTPH
                     VADVLSFVQPGQNQHPITAMVANRTYGNAPGQSSYGLPYQPENLGAGSVYKIFTAATA
                     MEQGLIGIDSVIDVPASGYLSPIYLDGSGHPTPVRNAEQGLAPQLSLTDALAQSPNTA
                     FVKLEESTGIAPVVDMAQRLGLTSLSDPVSNKPGALSIADTVKAQKQASFTLGVSPTS
                     DLELANVEATLQSHGMWCPPTPIASVTDQNGAPVPVNQLACKQAVAPALADTLVNGLS
                     HDDQPGGTSATSAAAEGWNRPTAAKTGTTEIYQAAAFAGSTPQLAGAAIVFDDSAHPR
                     PICDGSPPSTCSSGSLYGGGAPAR"
     gene            2172..2372
                     /gene="tatA"
                     /locus_tag="VFE39_10750"
     CDS             2172..2372
                     /gene="tatA"
                     /locus_tag="VFE39_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HET6259119.1"
                     /translation="MGELSPWHWLIVIGVVVLLFGAKRLPDAARSLGRSARILRAELQ
                     AGTAPPATEPDVSRPENDSTKE"
     gene            2410..2709
                     /locus_tag="VFE39_10755"
     CDS             2410..2709
                     /locus_tag="VFE39_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6259120.1"
                     /translation="MQAVLEALVEPRRREILRLVRDGELPAGAIAEQFPDVARPTVSQ
                     HLRVLRGAGLLVERREGTRRFYRARPDALAELRAFVEDFWEARLADIQTIAEKGP"
     gene            2732..3022
                     /locus_tag="VFE39_10760"
     CDS             2732..3022
                     /locus_tag="VFE39_10760"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HET6259121.1"
                     /translation="MRLVSAAGTTFLDDLGVAGSRGALFPAFSVHTRDGNGIRHVLTQ
                     PADFPDGTPRGMDLLTPVWNLLDLLPSGRGDREPDNTYPVAPAAHAGGRSGA"
     gene            3019..3216
                     /locus_tag="VFE39_10765"
     CDS             3019..3216
                     /locus_tag="VFE39_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259122.1"
                     /translation="MTRWLPPVLGALLLIIGAVWTLQGANLLGGSFMSGSRMWLVIGL
                     VVLIAGVALLSRSLRGRRTRR"
     gene            3281..3931
                     /locus_tag="VFE39_10770"
     CDS             3281..3931
                     /locus_tag="VFE39_10770"
                     /inference="COORDINATES: protein motif:HMM:NF025683.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HET6259123.1"
                     /translation="MNKVRRDSRWRLDAVHRVGAVLFGLGLWVFGILGIVDRLKPFST
                     SGESVLGLSSNGLLSAISLIVGGVLIAAALRGGRTASTVTVVVGAAFVLSGLVNVLVL
                     NTPLNLLAFRMPNVIFSLVSGALLLFLGAYGRFTGGLPADNPYQAERHGGGDLEVPLP
                     TVFRDQADVVAARELAEAERAVAQHAATREQVVRVAAARGARRAEDRVSEWRAGSG"
     gene            4177..4323
                     /locus_tag="VFE39_10775"
     CDS             4177..4323
                     /locus_tag="VFE39_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259124.1"
                     /translation="MALLLARLPQLPQAVLTTRGRTAAHAVDTGKSTVHPVIAAAASS
                     IARS"
     gene            4631..5917
                     /locus_tag="VFE39_10780"
     CDS             4631..5917
                     /locus_tag="VFE39_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6259125.1"
                     /translation="MAWDFSTEPEFEAQLEWMRGFVRTEIIPLETLDLDRETFARITA
                     PLKEQVKERGLWAAHLPPELGGGGFGQVRLGLMHEILGQCRYAPGIFGNQAPDSGNAE
                     LLAIGGTPEQKERFMHPLLRGELRSCFSMTEPGAGADPTLLSTRAVLHGDEYVINGHK
                     WFSSNASHADFLIVMAVTDPDVSAYQGSSMFIVPVDTPGVQIVRDIPVMDHPTIGGQL
                     YGGHAEVLYTDVRVPKENLVGNPGDGFRLAQQRLGPGRIHHAMRWLGQSKRAFDMMCE
                     RAVSRYTHGSVLADKQMVQDWVALSAAEMQAARLMTLHAAWTMDQVGAANARVEIAMI
                     KYWGATVLHDVIDRAIQVHGSLGFSGDLPLESMYRAARAARIYDGPDEVHKATVARRL
                     LRGYTPVDVPSEHVPTRTEAARKKFAEYLEGATANL"
     gene            complement(6014..6991)
                     /locus_tag="VFE39_10785"
     CDS             complement(6014..6991)
                     /locus_tag="VFE39_10785"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6259126.1"
                     /translation="MRIAKRLGRDVGVVGLGCWQLGADWGHVEESEALAVLHGAADTG
                     VTFFDTADVYGDGRSETLVGRFLRERGDARLTVATKMGRRVAQAPENYTRENFRAWND
                     RSRANLGVDTLDLVQLHCPPTPVYSSGEVYDALDELVADERIAAYGVSVERVEEALTA
                     ISRPGVASVQIILNALRQKPLEQVLPAAADAGVAIIVRVPLASGLLSGRYTAATTFDA
                     DDHRTYNRHGEAFDVGETFAGVPFEVGLQAVERLRPLVPAEATMAQFALRWIVDQPQV
                     TVVIPGARNAEQARGNAAAAELAPLGEQVHAAVREIYDELVRPHVHDRW"
     gene            7053..7892
                     /locus_tag="VFE39_10790"
     CDS             7053..7892
                     /locus_tag="VFE39_10790"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HET6259127.1"
                     /translation="MRLVPAVIGMAAVVVAACGQPTAAPGGEGGPVDVAALVGSWSVT
                     ATATADTMLVLHPDGFELRSGGPAVRYGSWRADSSGLFVAHVTGASGGARPDMSTPPW
                     LEAATTVTAAAGDRLLLDAAGRTTAQLSPRAWQPPAPGGPTGRSGPAAPLPAGLEPVE
                     RSRLIGRWEIADHERAALAPQPPHVDLAPDGAWTGTDGCNNGAGRWTTGPAGALLATS
                     GPMTLMACAGMAPVPGWLGQTARAGFDGATLVLVDADGRELGRLVRGPAAGSARLAPL
                     PRG"
     gene            complement(7860..8045)
                     /locus_tag="VFE39_10795"
     CDS             complement(7860..8045)
                     /locus_tag="VFE39_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259128.1"
                     /translation="MRWWKWLGLAGLAGVAATGVVTARAERRRRSYTPEEIRSRLHER
                     VAQARTGSAAGERREPG"
     gene            8065..8802
                     /locus_tag="VFE39_10800"
     CDS             8065..8802
                     /locus_tag="VFE39_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259129.1"
                     /translation="MPGSVITLDRACELAGTGDVWVFRGSSTADRAIRGLTNAPVNHV
                     GMAIVVDDLPPLMWHAELGRSLPDRWSGAHQRGVQLHDLRDAVLQWGHRYGQHAWLRQ
                     LAGPLSREMEDAALRTVARLDGTPFPSTARLAGRWLRGRAPVLRRPGREGADAPGLET
                     AYCAEVVAITYEEMGLLAEHRIPSWYDPGRFWSGDELDLAPGFHLGGEIAVALPPAPG
                     TTNVPVDAAHRRTWRPRRRLPERRSNV"
     gene            complement(9207..9956)
                     /locus_tag="VFE39_10805"
     CDS             complement(9207..9956)
                     /locus_tag="VFE39_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6259130.1"
                     /translation="MGLEVERCGRILLVRMARPAKRNAIDGSMTTALDAALNELDDDP
                     ELWVGVLSGTPAAFSAGTDLVAGAGAATERGGNYGVVGRDRNTPLIAAVEGIAYGGGF
                     ELVLACDLVVAARTARFALPETARGLVANCGALFRTQRALPRNIATEMLLTGEPLGAE
                     RAWTLGLVNTLVEPGEAEAAALSLAERVCANAPVAQRATLRALADMVADDDARGWSAT
                     ERAQLTVNMSQDAIEGVEAFREKRAPRWNGR"
     gene            10237..10572
                     /locus_tag="VFE39_10810"
     CDS             10237..10572
                     /locus_tag="VFE39_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lsr2 family protein"
                     /protein_id="HET6259131.1"
                     /translation="MAKQTTVTLVDDLDGSEAEEQVEFAFEGKAYEIDLSAANSARLR
                     SALEPFVSAARRTGGRRRSGGTAVATRPSTDREQNQAIREWAQEQGMKISERGRIPSN
                     VLEAYHQSH"
     gene            10628..11686
                     /locus_tag="VFE39_10815"
     CDS             10628..11686
                     /locus_tag="VFE39_10815"
                     /inference="COORDINATES: protein motif:HMM:NF022694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3047 domain-containing protein"
                     /protein_id="HET6259132.1"
                     /translation="MTQPAAVRMVLPGSSPPWTSTGTEVCRGDRVTLLGSGRINWSES
                     HWGGAKYHLWGRVPGGEVFGCTQDTTTVVVDRSGPLELCVYLGAWADRSGTLVTGACL
                     DDRSSYGRERGLLAVTLLHWPQGVDPVDGLAALEPGVADPRLVAAERRRLLDPVVPPA
                     GWSYLLAAGPGDIYRHAVAEGRPAIEVVCDDDAGVIEKDVDMALRSDTTIEWSWRVES
                     LPGLVPENTAWTHDLLGIAAVFDTGFPTPGTPVSSLNWFWSSALQPEDDTFDCPVPGW
                     TEHETHLPVRRGPAGLGRWRRETRAVRVDHERFMGPAPTRIRSLRLVAVSHFDHGCGR
                     ATFRDIILRSGGHRIQVL"
     gene            11780..12160
                     /locus_tag="VFE39_10820"
     CDS             11780..12160
                     /locus_tag="VFE39_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6259133.1"
                     /translation="MTTTTPSTTLVHAYLAAWNETDPIARRGAIEAAFAPDVRYVDPL
                     ADVRGTDALDALIAGAQQQFPGMEFRPVGDVDAHHDVCRFQWGLGPAGAEPLVIGFDV
                     AAIAADGRITTIHGFLDRVPATAS"
     gene            complement(12230..12991)
                     /locus_tag="VFE39_10825"
     CDS             complement(12230..12991)
                     /locus_tag="VFE39_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HET6259134.1"
                     /translation="MDITDGVALVTGGASGLGLATTKALVAAGAKVVILDLPSSPGQE
                     RATELGDAVRFAAGDVRSEADVVAALDVADALGPLRVAVNCAGTANAIRVIGKKGVFP
                     LAEFTRIIEINLIGTFNVLRLAAERMVAHDPVGEERGVIVNTASAAAFDGQIGQAAYS
                     ASKGGVVGMTLPIARDLASMSIRVVTIAPGLFDTPLMAILPEEARASLGAQVPHPSRL
                     GDPAEFGALAAHIVANPMLNGEVIRLDGAIRMGPR"
     gene            complement(13044..13592)
                     /locus_tag="VFE39_10830"
     CDS             complement(13044..13592)
                     /locus_tag="VFE39_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6259135.1"
                     /translation="MLTAADELDSGAVGLRRWRRADADDLYRAVSESLEHLAPWMAWA
                     ARGYSTADAVAYLARTEELWGTDYDYGIVAPGPALVGSCSMMSRIGAGGFEIGYWVHP
                     AHIRRGYATAAAAALVAEAFRVGADRVEIVHDLANIASGAIPRRLGFREVDRRTPQEE
                     RSAGEVGVDVVWRLGATDAAMV"
     gene            complement(13656..14861)
                     /locus_tag="VFE39_10835"
     CDS             complement(13656..14861)
                     /locus_tag="VFE39_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259136.1"
                     /translation="MSIEAHSGVGERGRLRLLQLAALTSTCDRFAIAPLLVVIGLDLG
                     APLSAVAGVASAYFLAYGLMQPVWGMISDRIGRVRVMRLALLGATLAGVGSMLAPNLL
                     VLGVMRTLAGGCFAALIPATLVYVGDMWPPASRQRPLSDVLAASSLGIAVATAGAGLL
                     ADLFHWRVVPAVTGAAAALLWIALARLPEPDRGPATGNPLRSVARVLRNRWALVVYAL
                     VFVEGIAVLGLLTYLAPAVQALGASAALAGLVAASFGVGALGWSRIVRLLVGRLPPGG
                     LAAIGGALLIAGWAVPSVVVTLPTVAVAGLLLGGSWAFLHTTLQSWATEVVPTERATA
                     VALFAALLFLGSSAGAAAAGPLADAGAFGTVFRVALGLAVPLALAAAVARTRYGGAGG
                     PPSAAPGAE"
     gene            15002..>15198
                     /locus_tag="VFE39_10840"
     CDS             15002..>15198
                     /locus_tag="VFE39_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259137.1"
                     /translation="MRRTIFGLIALLLLAACAAPAAEAPPDLSGDWHGSIETPGAPLQ
                     IGVRLAGDGGTFDLPAQGLRAL"
BASE COUNT         2168 a         5557 c         5328 g         2145 t
ORIGIN      
        1 cgggccggcg caccgccgcc gtagagagag ccgctcgagc aggtgctggg cggcgacccg
       61 tcgcagatcg gacgcgggtg cgcggagtcg tcgaacacga tcgccgcgcc ggcgagctgc
      121 ggcgtgctgc cggcgaacgc tgcagcctgg tagatctccg tcgtccctgt cttggccgcg
      181 gtcggccggt tccagccttc cgccgcggcc gatgtcgccg acgtgccgcc cggttggtcg
      241 tcgtggctga ggccgttcac cagggtgtcg gccaacgccg gagcgaccgc ctgcttgcag
      301 gcgagctggt tcaccggcac cggcgcgccg ttctggtccg tcaccgaggc gatcggcgtc
      361 ggcgggcacc acatgccgtg gctctgcagc gtggcttcga cattggccag ctccaggtca
      421 ctcgtgggcg acaccccgag ggtgaacgat gcctgcttct gcgccttcac cgtgtcggcg
      481 atcgacaggg ctcccggctt gttgctcacc gggtcggaca gcgaggtcag cccgagccgc
      541 tgcgccatgt ccaccaccgg tgcgatcccg gtgctctcct cgagcttcac gaacgcggtg
      601 ttgggcgact gggcgagcgc gtcggtcagc gagagctggg gggcgaggcc ttgctcggcg
      661 ttgcgtaccg gcgtcgggtg gcccgacccg tccaagtaga tcggggagag gtagccgctc
      721 gccgggacgt cgatcaccga gtcgatgccg atgaggccct gctccatcgc ggtggcagcg
      781 gtgaagatct tgtagaccga gcccgcaccc aggttctccg gctggtaagg cagcccgtag
      841 ctggactgcc cgggcgcgtt gccgtatgtg cggttggcca ccatggcggt gatcgggtgc
      901 tggttctggc cgggctgcac gaaggagaga acgtcggcga cgtgcggcgt ggtcggcggc
      961 acctgactgt tcaccgcggc cttggccgtc gcctgtgcct gcgggtccag cgtggtgcga
     1021 atcgtgtagc cgcccgacga gagctgctgg gaggagaacc cggcctgctc caggtagcgc
     1081 acgacgtagg aacagaagta gccgttatcg cccgcagccg tgcagccctc ggccggtact
     1141 gccgcagcgg actgcacgcc cagcggcgcc gcggtggcgg cggccgcgtc cgcatcggtg
     1201 atcgagccca cctggcggag ctgggagatc accaggtctc gtcgcgactt ggccgcgtcg
     1261 ggatgctgga tcgggtcgaa ctgggtgggg ctctgcacca tcccggcgag caacgcggcc
     1321 tgcggaatgg tcagcgccgc tgcggtggtg ccgaagtaga cctgggctgc cgccgcgacg
     1381 ccgtaggcgc cgtgcccgaa gtagacgatg ttgaggtagc gggtgaggat ctgatccttg
     1441 ctgagctcct ggtcgagctg gaccgcaagc tcggcctcgt ggatcttgcg ggcgaggctc
     1501 ggcgccacag ccgcctggcg ctgcgcgtcg gtggtggccg ccacgtacag ctcgaagttc
     1561 ttcacgtatt gctgcgtgag cgtcgagcca ccttgttgag ccccgccacc tgcgtcgttg
     1621 acgagggcgc gcagggtgcc gaccggatcg atggccccgt gttcgaagaa acgcttgtcc
     1681 tcgatggcga cgatcgccgc tttcatcgcc ggagagatct gcgcactcgt ggcggggacc
     1741 cggaactggt catagaggta cgcaatgggc tttccggtgg aatcggtcac cgtcgtgacg
     1801 ttcggtgtgt tcccggcaag tagagcggac ggtgcgacat tgaccgaacc actgctgacc
     1861 tgcgccgcca gcgcaccagc gccgcccgcc actggcaagc tcatcaccgc cagcaccact
     1921 gctgccacca gtcccagtac caccagcttc gcgcacctgc gaacaactcc cacgccacaa
     1981 catctaccag cagtgacgct gggtgcgcag cgcttggacg aatgagcagg aacttccggt
     2041 aacggaccag cgaacccttg gggaggaacc gccgaaacgc tccccagtat atgcccgggc
     2101 ttctgcggaa gtgcacactc gacatgttca ggtgacgcac cgacagcaca gcattgcgaa
     2161 aggcgggaga aatgggcgag ctcagcccct ggcactggct catcgtgatc ggcgtagtgg
     2221 ttcttctgtt cggcgcgaaa cgactgcccg atgcagcgcg cagcctcggc cggtcagcac
     2281 ggatcctccg ggcggagctt caggccggca ccgcccctcc agctaccgaa ccggatgtga
     2341 gccgaccgga gaacgactcg accaaagagt aggcttccac ctacgtaatc gatggcctac
     2401 gctggtccga tgcaggccgt cctcgaagcc ctcgtcgaac cgcgcaggcg cgaaatcctg
     2461 cggttggtgc gcgacggcga actgccggcg ggcgccatcg ccgagcagtt ccccgacgtc
     2521 gcccggccca cggtgtcgca gcacctgcgc gtgctgcgcg gcgccgggct gctcgtggaa
     2581 cgccgcgagg gaacgcggcg gttctaccgc gcccgacccg acgcgctcgc cgagctgcgc
     2641 gccttcgtcg aggacttctg ggaggcccgg ctggccgaca tccagacgat cgccgagaag
     2701 gggccgtgat cacagaagcc ggtggtcacg ggtgcggctg gtgtccgcgg ccggcacgac
     2761 attcctcgac gacctcgggg tggcggggtc gcgcggcgcg ctgttccccg cgttcagcgt
     2821 ccacacccgc gacgggaacg gcatacgcca tgtcctcacc cagcccgccg acttccccga
     2881 cggcaccccg cgcggcatgg acctgctgac ccccgtgtgg aacctgctcg acctgttgcc
     2941 atccggtcgc ggcgaccgcg agccggacaa cacctatccg gtcgcccctg cagcgcacgc
     3001 aggaggacga tcgggcgcat gacccgatgg ctcccgcccg tgctcggcgc gctcctgctc
     3061 atcatcgggg cggtgtggac attgcagggg gccaacctgc tcgggggcag cttcatgagc
     3121 gggtcacgaa tgtggctcgt gatcgggctg gtggtgctga tcgccggggt cgcgttgctc
     3181 tcccgctcgc tgcggggccg ccgcacccgc cgctgagctg ttcgagggcg gcagggcaga
     3241 tcaactggcg agaggtgccg gttcggttca ccatcagttc atgaacaagg tacggcgcga
     3301 ttcgcgttgg cgcctcgacg cagtgcaccg cgtcggtgca gtgctgttcg gcctcggact
     3361 gtgggtcttc ggcatcctcg gcatcgtcga ccggctcaag ccgttctcga ccagcggcga
     3421 gtcggttctg gggctgtcgt ccaacggcct gctgtccgcg atctcgctga tcgtgggcgg
     3481 ggtgctgatc gcggcggcgt tacgtggcgg ccgcacggcg tccacggtga cggtcgtcgt
     3541 gggagccgcg ttcgtcctgt ccggcctggt caacgtgctc gtcctcaaca cgccgctcaa
     3601 cctgctcgcg ttccggatgc ccaacgtgat cttcagtttg gtctccggcg ctctcctgct
     3661 gttcctgggc gcgtacggcc gcttcaccgg tgggcttccc gcggacaacc cctaccaggc
     3721 ggaacgtcac ggcggcggcg acctggaggt tccgcttccg accgtgttcc gggaccaggc
     3781 cgacgtggtc gccgcacggg agctcgccga ggccgagcgc gccgtcgccc agcacgccgc
     3841 tactcgcgag caggtggtgc gcgtcgccgc tgcccgcggg gcgaggcggg ccgaggaccg
     3901 ggtcagcgag tggcgggcgg gctccggctg atccgagcgg ctgatccggg tggaccggct
     3961 cagcgggtct ccgggcggcg caacaggccg cggatacagg ccgcgagcca ccacgcttcg
     4021 acgcgttccg gcgcccagcc gcgcccgtcc acccagtcgc gcgtacaggt cgggcgccat
     4081 gtagagccac acctggtcga tggcgcttcc gaggtcgaaa ccgtcagtgg cctgccgctt
     4141 cccggcgagc atcgtgatcc acatcgtggc gccggcgtgg cgctgctgct cgctcgcctc
     4201 ccacagctcc cgcaggccgt cctcaccact cgcggccgca ccgcggcgca cgccgtcgat
     4261 accggcaaat cgacggttca cccggtgatc gcggcggcgg cgagctcgat cgcgcggtcc
     4321 tgatcgggct cgtcccgtac atggccgcgg ctgctattgg gaccacctgc ggtgcggctg
     4381 ggtctgcgcg tccacccgcg cagcgccgga gaccgaggcg cagccggagg tgccctcgcc
     4441 cgcacccgag ccggctcccg cacccgagcc gctgaccggc acgcgctgaa ccgcacgagt
     4501 cggtctgcgc cgctgtcgat ttgcgccgct gtcgatctgt gctgctgtcg atctgtgctg
     4561 gcggcggaag ccggggcacg atgggcccgg gggtcggccg gccctcggcg acgatgcgga
     4621 ggcgcggacg atggcgtggg acttctcgac ggaacccgag ttcgaggctc agctcgagtg
     4681 gatgcgcggc ttcgtccgca cggagatcat ccccctggag accctcgacc tcgaccggga
     4741 gaccttcgcc cggatcaccg ctccgttgaa ggagcaggtg aaggagcgtg gcctgtgggc
     4801 cgcgcacctg cctcccgaac tcggcggtgg tgggttcggg caggtcaggc tcgggttgat
     4861 gcacgagatc ctcgggcagt gccgttacgc gccggggatc ttcggcaacc aggcgcccga
     4921 ctccggcaac gccgagctgc tggccatcgg cggcacgccg gagcagaagg agcgcttcat
     4981 gcacccgctg ctgcgcggcg agctgcgtag ctgcttctcg atgaccgaac ccggcgcagg
     5041 cgcggacccg acgctgctgt ccacccgggc tgtgctccac ggggacgagt acgtgatcaa
     5101 cggccacaag tggttctcgt cgaacgcatc acacgccgac ttcctgatcg tcatggctgt
     5161 caccgatccg gacgtgtcgg cctaccaggg ctcgtcgatg ttcatcgtcc cggtcgacac
     5221 acccggggtg cagatcgtgc gcgacatccc ggtgatggac cacccgacca tcggtgggca
     5281 gctctacggc gggcacgccg aggtgctcta caccgacgtg cgggtgccga aggagaacct
     5341 cgtcggcaac cccggcgacg gcttccggct cgcccagcag cggctcggtc ccggtcgcat
     5401 ccaccatgcg atgcgctggc tcggacagtc caagcgggcg ttcgacatga tgtgcgagcg
     5461 cgccgtcagc cgctacacgc acgggtcggt gctggccgac aagcagatgg tgcaggactg
     5521 ggtggccctg tcggccgccg agatgcaggc ggcccggctg atgacgctgc acgccgcgtg
     5581 gacgatggac caggtcggtg ccgccaacgc ccgcgtggag atcgccatga tcaagtattg
     5641 gggcgcgacg gtgctccacg acgtgatcga ccgagcgatc caggtgcacg gctcactcgg
     5701 gttctccggc gacctgccgc tcgagtcgat gtaccgggcc gcccgcgccg cccggatcta
     5761 cgacgggccg gacgaggtgc acaaggccac ggtggcccgc cggctcctgc gcgggtacac
     5821 ccccgtcgac gtcccgtccg agcacgttcc gacccgcacg gaggcggcgc ggaagaagtt
     5881 cgcggagtac ctggagggag ccaccgcgaa cctgtgacgg ccccgggccc ggccccgcgg
     5941 cgcgaacggc gcggtcgcac gcatccggcg tacgagagtg ccgttcgtcc gacgggccgg
     6001 gcgccgggcg gggtcaccag cggtcgtgca cgtgggggcg caccagctcg tcgtagatct
     6061 cgcggacagc ggcgtgcacc tgctccccca ggggtgcgag ctccgctgcg gcggcgttgc
     6121 cgcgcgcctg ctcggcgttc cgggcacccg ggatgaccac ggtgacctgc ggctggtcga
     6181 cgatccagcg cagcgcgaac tgcgccatcg tcgcctcggc aggcaccagc ggccgcagcc
     6241 gctccacggc ctgcagcccc acctcgaacg gcacgccggc gaacgtctcg cccacgtcga
     6301 aggcctcgcc gtgccggttg taggtgcggt ggtcgtcggc gtcgaacgtg gtggcagcgg
     6361 tgtagcgccc cgacagcagc ccggacgcga gcggaacccg cacgatgatc gcgacgccag
     6421 cgtcggcggc cgcaggcagc acctgctcca gcggcttctg gcgcagcgcg ttgaggatga
     6481 tctgcacgct cgccaccccg ggccgggaga tcgcggtgag cgcctcctcc acccgctcga
     6541 cgctcacccc gtacgccgcg atccgctcgt cggccaccag ctcgtcgagc gcgtcgtaga
     6601 cctcgcccga ggagtagacg ggcgtgggtg ggcagtgcag ctgcaccagg tccagcgtgt
     6661 cgacgccgag gttggcccgc gaccggtcgt tccaggcccg gaagttctcc cgcgtgtagt
     6721 tctccggcgc ctgcgcgacg cgccgcccca tcttcgtcgc caccgtgagc cgcgcgtcgc
     6781 ctcgctcgcg caggaaccgg ccgacgagcg tctcgctgcg gccgtcgccg tagacgtccg
     6841 ccgtgtcgaa gaacgtgacg ccggtgtccg cggcgccgtg cagcaccgcg agggcctcgc
     6901 tctcctcgac gtgaccccag tccgcgccca gctgccagca ccccaacccg accacgccga
     6961 cgtcacgacc gaggcgcttc gcaatccgca tctccacgga ccgagcctag gcttcgtgca
     7021 acccgttgcc ccctcggctc cgtcctaggg tcatgcgcct cgtgccggca gtgatcggaa
     7081 tggccgccgt cgtcgtggcc gcctgcgggc agccgacggc cgcacccgga ggagaaggtg
     7141 gacccgtgga cgtggccgcg ctcgtcggta gctggtcggt gaccgcgacc gcgaccgcgg
     7201 acaccatgct cgttcttcac cccgacgggt tcgagctgcg ctccggcggc ccggccgtcc
     7261 ggtacggcag ctggcgggcc gactcgtccg gcctgttcgt cgcgcacgtc acgggtgcca
     7321 gcggtggggc gcgtcccgac atgtccacgc cgccctggct ggaggccgcc accaccgtca
     7381 cggccgcggc cggcgaccgt ctgctgctcg acgcggcggg caggacgacg gcgcagctgt
     7441 ccccgcgggc ctggcaacca ccggcccccg gcgggcccac cgggcgatcc ggcccggcgg
     7501 cgccgctccc ggccggcctg gagcccgtcg agcgttcacg gctgatcggg cgctgggaga
     7561 tcgcggacca cgagcgggca gccctggcgc cgcagcctcc acacgtcgac ctggccccgg
     7621 acggtgcctg gaccggcacg gacggctgca acaacggtgc cggtcggtgg acgaccggcc
     7681 cggcaggggc cctcctcgcc acgagcggtc cgatgacgct catggcctgc gcgggtatgg
     7741 cgcccgtccc cggctggctg gggcagaccg cacgcgccgg gttcgacggc gcgacgctcg
     7801 tgctggtcga cgcggacggc cgcgagctcg ggcggctggt ccgaggcccg gccgccggat
     7861 cagcccggct cgcgccgctc ccccgcggct gacccggtcc tggcctgggc cacccgctcg
     7921 tgcagccggg accggatctc ctcgggcgtg taggaccgcc tgcgccgctc ggcccgcgcc
     7981 gtgacgacgc cggtggccgc cacgcccgcg aggccggcga gccccagcca cttccaccac
     8041 cgcatggcgg ttaacctacc ggccgtgccg ggcagcgtga tcaccctcga ccgggcgtgc
     8101 gagctggccg gcaccggcga cgtctgggtg ttccgcggca gcagcacggc agaccgcgcc
     8161 attcgcggcc tgacgaacgc accggtcaac cacgtcggca tggccatcgt cgtcgacgac
     8221 ctgccgccgc tgatgtggca cgccgagctc ggccggtcgc tgccggaccg gtggtcgggc
     8281 gcgcaccagc gcggcgtcca actgcacgac ctgcgcgatg cggtcctgca gtgggggcac
     8341 cgctacgggc agcacgcctg gttgcggcag cttgcgggcc cgctcagcag ggagatggag
     8401 gacgcggcgc tgcggacggt ggcgcggctc gacggcaccc cgttcccctc caccgcccgc
     8461 ctggccgggc ggtggctgcg gggccgggcg ccggtgctgc gccgccccgg ccgcgagggc
     8521 gcggacgcac ccggtctgga gacggcctac tgcgccgagg tcgtggccat cacctacgag
     8581 gagatgggtc tgcttgccga gcatcgcatc ccgagctggt acgacccggg gcggttctgg
     8641 agcggcgacg agctggacct ggcacccggc ttccacctcg gcggggagat cgcggttgca
     8701 ctcccgcccg cgcccggcac gacgaatgtc ccggtcgacg ccgcccaccg gcggacttgg
     8761 cgcccgcgcc gccggctccc tgagcgacgg tccaacgtct gagaacgtct gttcacatcg
     8821 gctgatctgt cacactcagt cgccagaatc accgctcgga tacgggctgc aatcgccgcc
     8881 ggccgacagc gaagaatccg ggcccgatgc atggcacggg cctcggtgcg gttggttgcg
     8941 ggaggacctc cggcacggcg ggcaagcgtc tcggcatccc gcgccaagcg ctccgaggca
     9001 cttgtgaatc tcctgcgcca aacggtcgcc tctcggcagc tgcgcgaagc ccgtcgtttc
     9061 cggtgcccga ccggggtgga cgcccggatt cgttgtgcat gccgagccgc tgtgccgaca
     9121 tgccccggac tgcatgagga catcaccatc tcgggccatg cccgcggagg agtgcgttgg
     9181 tcgcatccgt attgcgccgg cgccactcac cgaccgttcc agcggggagc gcgcttctcg
     9241 cggaacgctt caacgccctc gatggcatcc tgcgacatgt tgaccgtcag ctgagcgcgt
     9301 tcggtggcgg accagcctcg ggcgtcgtcg tcggccacca tgtcggccag cgcccggagg
     9361 gtggcgcgct gggcgaccgg cgcattcgcg cagacgcgct ccgcgagcga gagcgcagct
     9421 gcctctgcct cgcccggctc caccagcgtg ttcaccagcc cgagcgtcca cgcccgttcg
     9481 gctccgagcg gctctccggt cagcagcatc tcggtcgcga tgttgcgcgg cagggcccgc
     9541 tgcgtgcgga atagtgcccc gcagttcgcc acgagcccgc gcgccgtctc gggcagggcg
     9601 aaccgcgccg tccgtgctgc aaccacgagg tcgcaggcca gtacgagctc gaacccaccg
     9661 ccgtaggcga tgccctcgac ggcggcgatc agcggcgtgt tccggtcccg cccgaccacg
     9721 ccgtagttgc ccccgcgctc agtcgcggca cccgcaccgg ctacgaggtc ggtgcccgcg
     9781 gaaaaggcgg ccggcgtccc cgagagcact cccacccaca gctccggatc gtcgtccaac
     9841 tcgttgagcg cagcgtcgag agcggtcgtc atactcccgt cgatcgcgtt gcgcttggcc
     9901 ggacgcgcca tccggaccag cagaatccgt ccacatcgct cgacctcgag acccacgggt
     9961 caagcctagt cagcaggggc gcagcgttgg cactggtggc ctgaccggct ctttttcggt
    10021 cggcggggat cccattcggc cgggaatggc ctggcccacg gggcagccgg cgggcccagg
    10081 ccgcaccgat ccatccgacc ccgatcgaca atgggcaccc gaatccttta ttatcaacca
    10141 cgcaattgga gcccaccccc gttcggaacc gtggaaccct cgtaccacat cgagggcacc
    10201 ccggcgtacc cggagcaccc tggaaaggca gcgcagatgg ctaagcagac gacagtcacc
    10261 ctggtcgatg atctcgatgg cagcgaggcg gaggagcagg tcgagttcgc gttcgagggg
    10321 aaggcctacg agatcgacct ctcggccgcc aatagcgcca gattgcgctc ggccctcgag
    10381 ccgttcgttt ctgccgcccg ccgcaccggc ggccgccgcc gttcgggcgg caccgccgtc
    10441 gccacccggc ccagcaccga ccgcgagcag aaccaggcca tccgggaatg ggcacaggag
    10501 caggggatga agatctccga gcgcggccgc atcccgtcca atgtgctcga ggcctaccac
    10561 cagagccact gacgggcaga acaactgatc gggcccgcca cgccgcagga tctgcgaagg
    10621 tggggcgatg acccaaccgg cagccgtgcg gatggtgctg ccgggcagta gtccgccgtg
    10681 gaccagcacc ggtaccgagg tgtgccgcgg cgaccgcgtc acattgttgg gctcgggccg
    10741 catcaattgg tcggagagcc actggggtgg ggcgaagtac cacctgtggg gccgcgtccc
    10801 gggcggggag gttttcggct gcacccagga caccaccacc gtggtcgtcg accgttcggg
    10861 accgctggag ctctgcgtct acctgggcgc gtgggccgat cgttccggca ctctcgtcac
    10921 gggcgcgtgt ctcgacgacc gcagctccta cgggcgggag cggggcttgc tggcggtcac
    10981 cctgctgcat tggccgcagg gcgtcgatcc ggtcgacggg ctggcggcgc tcgagccggg
    11041 cgtcgccgat ccgcggctcg tcgccgccga gcggcgtcgg ctgctcgacc ccgtcgtccc
    11101 accggcgggc tggtcctacc tgctggctgc cggaccaggc gacatctacc ggcacgctgt
    11161 ggccgagggc aggccggcga tcgaggtcgt ctgcgacgac gacgccgggg tgatcgagaa
    11221 ggacgtcgac atggccctgc ggagcgacac gacgatcgag tggagctggc gggtcgagtc
    11281 gctgccgggc ctcgtcccgg agaacaccgc ctggacgcac gacctgctgg gcatcgccgc
    11341 agtgttcgac accggattcc cgacccccgg caccccggtg tcgagcctca actggttctg
    11401 gagcagcgca ctgcagcccg aggacgatac gttcgactgc cccgtcccgg gctggaccga
    11461 gcacgagacg cacctgccgg tgcgccgggg cccggcgggg ctcggccggt ggcggcggga
    11521 gacccgcgcc gtgcgcgtgg accacgagag gttcatgggc ccggccccga cacgcatcag
    11581 atcgttgcga ctcgtcgcgg tgagccactt cgaccacggc tgcggccggg cgacgttccg
    11641 ggacatcatc ctgcgtagcg gcgggcaccg catccaggtg ctctgacggc cagccttacc
    11701 tccgaggtga tcgacccggc tccccctgcc gggcgaggct ctccctgtcg ccatcaactc
    11761 gacatccgga ggatccccga tgaccaccac tacacccagc accactctgg tccatgccta
    11821 cctggcagcg tggaacgaga ccgatccgat cgcccgccgc ggcgcgatcg aggccgcctt
    11881 cgcgccggac gtgcgctacg tcgacccgct ggccgacgtc aggggcacgg acgcgctgga
    11941 cgcgctgatc gccggggcgc agcaacagtt cccgggcatg gagttccggc cggtcggcga
    12001 cgtcgacgcg caccacgacg tctgccgatt ccagtggggc ctcggcccgg caggcgccga
    12061 gccactggtg atcggcttcg acgtggccgc gatcgcggcc gacggccgga tcaccaccat
    12121 ccacggcttc ctggaccgcg tgcctgccac ggcatcctga tccacttccg ggctgcctcc
    12181 ggcggggccg gcggccccgc cggatctcgg cggcccgtcc ggcccatcgt cagcgcggac
    12241 ccatccggat cgcgccgtcg agccggatga cctcgccgtt gagcatgggg ttggccacga
    12301 tgtgcgcggc cagcgcgccg aactcggccg ggtcgcccag ccgggacggg tgcggcacct
    12361 gcgcgcccag tgatgccctg gcctcctcgg gcaggatggc catcagcgga gtgtcgaaca
    12421 ggcccggtgc gatggtgacc acccggatgg acatcgaggc caggtcacgg gcgatcggaa
    12481 gcgtcatgcc caccaccccg cccttggacg cggaataggc cgcctggccg atctggccgt
    12541 cgaaggcggc cgccgaggcg gtgttgacga tcacgccccg ttcctcgccc accgggtcgt
    12601 gcgccaccat tcgttcggcg gcgagccgca gcacgttgaa cgtgccgatc aggttgatct
    12661 cgatgatgcg ggtgaactcc gcgagcggga agacaccctt cttcccgatc acccggatcg
    12721 cattggcggt gccggcgcag ttgacggcca cccgcagcgg gcccagcgcg tcggcgacgt
    12781 cgagcgccgc gaccacgtcg gcctcgctgc gcacgtcgcc cgccgcgaac cgcaccgcgt
    12841 cgccgagctc cgtggctcgc tcctggcccg gcgagctcgg caggtcgaga atcaccacct
    12901 tcgctcccgc cgccaccagc gccttcgtcg tggcgagccc gagcccggac gcgcccccgg
    12961 tgaccagcgc gacgccgtcg gtgatgtcca cagattccgc tcctcgtcca aaccgtcaca
    13021 ggtgactgtt cctgcagtgg atgtcatacc atcgctgcat cggtggcgcc gaggcgccac
    13081 accacgtcga cacccacctc ccccgcgctg cgctcctcct gcggcgtccg gcgatccacc
    13141 tcacggaagc cgagccggcg cgggattgcc ccactggcga tgttggcgag gtcgtgcacg
    13201 atctcgacgc ggtcggcacc gacgcggaag gcctccgcga ccagcgccgc agcggcagcc
    13261 gtcgcatacc cgcgccgaat gtgtgcggga tgcacccagt acccgatctc gaaaccgcct
    13321 gcgccgatcc ggctcatcat cgagcagctg ccgaccagcg ccgggccggg ggcgacgatg
    13381 ccgtagtcgt agtcggtgcc ccagagctcc tcggtgcggg ccaggtaggc cacggcgtcg
    13441 gcggtggagt agccgcgcgc cgcccaggcc atccacgggg ccagatgctc cagcgactcg
    13501 ctcaccgcgc ggtacaggtc gtcggcatcg gcgcggcgcc accggcgcag cccgaccgca
    13561 ccggagtcga gctcgtccgc tgcggtcagc acgcccgcga ttctgccggg cggtcaccac
    13621 accgtgagca gcacgtggtc gaccgagagc ggcggctact ccgcgccggg tgctgcgctc
    13681 ggcggtccgc ctgcccctcc gtatcgcgtg cgggcgaccg ccgccgccag cgcgagcggc
    13741 accgccagcc ccagcgccac ccggaacacc gttccgaacg cccccgcgtc ggccagcggc
    13801 ccggccgccg ccgcgcccgc cgagctcccc aggaacagca gcgcggcgaa gagcgccacc
    13861 gcggtggccc gctccgtcgg caccacctcc gtcgcccagc tctgcagggt cgtgtgcagg
    13921 aacgcccacg acccgccaag cagcagcccg gcgacagcca ccgtcgggag ggtgaccacg
    13981 acggacggga ctgcccagcc ggcgatcagt aacgcgccgc cgatggcggc gagcccgccc
    14041 gggggcaacc tcccgaccag caaccgcacg atgcgcgacc acccgagcgc cccgactccg
    14101 aacgacgccg ccaccagccc ggcgagcgcc gccgatgccc ccagcgcctg cacggcgggc
    14161 gccaggtagg tgagcaggcc gagcaccgcg atgccctcga cgaacaccag ggcgtacacc
    14221 acgagcgccc atcggttgcg cagcacccgc gccaccgaac gcagcgggtt gccggtggct
    14281 ggtccgcggt ccggctccgg gagccgggcc agcgcgatcc agagcagggc cgccgcggca
    14341 cccgtcacgg ccggcaccac ccgccagtgg aacaggtcgg cgagcaggcc ggcccccgcc
    14401 gtcgcgaccg cgatgccgag cgaggacgcg gccagcacgt ccgacagggg gcgctggcgc
    14461 gaggcgggcg gccacatgtc gccgacgtag accagcgtgg ccgggatgag cgcggcgaaa
    14521 cagccgcccg ccagcgtgcg catcacgccg aggaccagca ggttcggcgc gagcattgat
    14581 ccgacgcctg cgagcgtcgc cccgagcagc gccaggcgca tcacgcggac ccgaccgatg
    14641 cggtcgctga tcattcccca caccggctgc atgaggccgt aggcaaggaa gtaggcgctc
    14701 gcgacgccgg cgacggccga caggggcgcg cccaggtcga gaccgatcac cacgagcagg
    14761 ggggcgatgg cgaaccggtc acaggtgctg gtcagggcgg ccagctgcag cagccgcaac
    14821 ctgccccgct cccccacacc ggagtgggct tcgatgctca cccgctgaca atgcttgagc
    14881 acggtcgcct gcgctgcccg aaagatggtg agaaccgcca ctacgacgcg tccgccgctc
    14941 cgtcgcgggc gagaggtgcc gacggtcggg cctcccgtcg accactcact acggtgtgcg
    15001 gatgcgacgg acgatcttcg ggctcatcgc gctgctgctc cttgccgcgt gcgccgcacc
    15061 ggcagcggaa gcacccccgg acctctccgg cgactggcac ggcagcatcg agacacccgg
    15121 ggcgccgttg cagatcggag tgcgcctggc aggcgacggc ggcacgttcg accttcccgc
    15181 ccagggactg cgcgcgct
//
LOCUS       DASRSC010000221        16339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2375398,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000221 DASRSC010000000
VERSION     DASRSC010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 16339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16339
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2375398"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VFE39_10845"
     CDS             complement(<1..250)
                     /locus_tag="VFE39_10845"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HET6259138.1"
                     /translation="MLGPIPIRAYAICIIVGIIVAVMWSERRFVARGGQPGTVTDVAV
                     FAVPFGLVGGRLYHVATDWETYFGPGGDPVGALKIWNGG"
     gene            complement(377..1189)
                     /gene="trpA"
                     /locus_tag="VFE39_10850"
     CDS             complement(377..1189)
                     /gene="trpA"
                     /locus_tag="VFE39_10850"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016335954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET6259139.1"
                     /translation="MSAVEAIFRESREQDRGTLVGYLPAGYPTVDGSVELFGALVEGG
                     CDLLEIGIPYSDPVMDGPTIQAAAETALRAGTKLRDVLSVVERVTAAGGRAVIMTYYN
                     PVLRYGVDAFARDLAAAGGLGMITPDLIPDEADEWFAASTAHGLDRIFLVAPSSSEER
                     IASTAAAGSGFLYATSTMGVTGARDSVASVAPELVARCRAHTTLPVGVGLGVRSADQA
                     AEVASFADGVIVGSAFVTAAARGGAGDVRALAAELAGGVRHAGRPSATPAPA"
     gene            complement(1263..2603)
                     /gene="trpB"
                     /locus_tag="VFE39_10855"
     CDS             complement(1263..2603)
                     /gene="trpB"
                     /locus_tag="VFE39_10855"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET6259140.1"
                     /translation="MSTPSGHEPDERGHFGRYGGRFVPEALIAALDELAAAYDKARGD
                     RDFLDELDRLHRDYSGRPSPLTDAPKLGEHAGGARILLKREDLNHTGSHKINNVLGQA
                     LLTQRMGKSRVIAETGAGQHGVATATACALLGLECVVYMGEVDTERQALNVARMRLLG
                     AQVVPVTTGSRTLKDAINEALRDWVTNVDRTHYLLGTVAGPHPFPTMVRDFHRIIGLE
                     AREQVLARTGRLPDAVAACVGGGSNAIGIFHAFLDDPGVRLVGFEPGGDGVETGRHGA
                     TLTEGSPGTLHGAMSYLLQDEDGQTAESHSISAGLDYPGVGPEHALLKDLGRAEYEPV
                     TDAEAMDAFALLCRTEGIIPAIESAHAIAGAVRLGRELGPDGIVLVNLSGRGDKDVDT
                     AAKWFGMIGLGESAEDASGTAIAEASLAGPGAAAPEHRPSGETPGTTEAEGVQR"
     gene            complement(2693..3517)
                     /gene="trpC"
                     /locus_tag="VFE39_10860"
     CDS             complement(2693..3517)
                     /gene="trpC"
                     /locus_tag="VFE39_10860"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET6259141.1"
                     /translation="MEHGGASVLDSIVDGVRADLAVREAEVDFAEIKRRAAATAPPRD
                     VMAALRAPGIGVIAEVKRRSPSKGDLAVIADPADLAVAYAGGGARVISVLTERRRFGG
                     SLADLAAVRAAVDVPVLRKDFVVSPYQVHEARAYGADLVLLIVAALEQNALDSLLDRV
                     ESLGMTALVEVHTEEEADRALEAGAKVIGVNARNLHTLEVNRSIFGQIAPGLPSDVLR
                     VAESGVRGPGDLLTYAGWGADAVLVGEGLVTSGNPGGAVRSLVAAGFHPSCSRMVR"
     gene            complement(3561..4148)
                     /locus_tag="VFE39_10865"
     CDS             complement(3561..4148)
                     /locus_tag="VFE39_10865"
                     /inference="COORDINATES: protein motif:HMM:NF021079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trp biosynthesis-associated membrane protein"
                     /protein_id="HET6259142.1"
                     /translation="MTQTRTRKARVLVAVCAGLAGSAALLWAASATVWYHGAGVAGRP
                     GTESTGAQVAPSLTGVALLALAAVAAVVAIGGVLRRLVGALLALVAVVAGVVAVRALV
                     VLPTAPDPVARTPAPLLAVAGAAALLAVGLFVLLREPRLPRFGSRYAAPGDRRAEVDP
                     DRAAWQALDAGMDPTADVPGSRGDDPGDGARTGPG"
     gene            complement(4145..5737)
                     /locus_tag="VFE39_10870"
     CDS             complement(4145..5737)
                     /locus_tag="VFE39_10870"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET6259143.1"
                     /translation="MTVTAPAPALGAVTPSREEFRALAVGHRVIPVTRRLLADDETPV
                     GVYRKLAGDRPGTFLFESAENGRSWSRWSFVGARSAAALTSVDGELVWTGEVPVGLPT
                     SGDPLAALRTVVEELRSEPLPGLPPLTGGMVGYLGYDVVRRLERLPELTTDDLQLPEL
                     MMLLAADLAAVDHHEGTITLIANAINWDARDERVDEAYDDAVARLDRMTTELAAPAAS
                     TVAVYQPVEPRFTRRRSPAEYHAAVEVVKEQIRDGEAFQVVVSQRFEAECPADPLDVY
                     RVLRATNPSPYMYLLRLESAGTSTHPARRFDIVGSSPEALVTVRDGKATTHPIAGTRW
                     RGATEEEDLLLEKELRSDEKERAEHVMLVDLGRNDLGRVCEAGTVKVHSFFSVERYSH
                     VMHLVSTITGTLRRDCNAFDAVIACFPAGTLSGAPKPRALQIIEELEPTRRGLYGGIV
                     GYLDFAGDADTAIAIRTAVVRDGIAYVQAGGGIVADSDPAAEDTESLNKARAVLSAVA
                     TAATLAEPPIGTDTAETPGPPR"
     gene            5825..6484
                     /locus_tag="VFE39_10875"
     CDS             5825..6484
                     /locus_tag="VFE39_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET6259144.1"
                     /translation="MAAPEEHSPPGARGAPVDAGSADAGPRRRGRRPGGADTRAQLLA
                     AARVEFADRGYDGATVRVIAERAGVDPAMVNHWFGGKESLFTAALDLPVDPGVILADV
                     VPGDPDHLGERIIARFLEIWDTTGGAPLAAVLQSVAGHDAAARMLRDFVSRVLVGKVV
                     STVAPDRPELRAGLCGSQVFGLAIIRYVLKVEPLASADHATVIGAVAPNLQRYLTGPL
                     P"
     gene            complement(6496..7155)
                     /locus_tag="VFE39_10880"
     CDS             complement(6496..7155)
                     /locus_tag="VFE39_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6259145.1"
                     /translation="MIKLLLVDDEELVRAGLRTILGAEPDITVVGEASDGADVPGLVR
                     RFAPDVVLMDVRMQAVDGIAATRHLVTTLPNPPRVLVLTTFGNDGHVYDALQAGATGF
                     LLKRARPAEIVQAVRTVAGGDSLLFPAAVRDLVATRGRMGGDALRSAGLTEREGEVLR
                     WVARGLSNAEIARALMVGVETVKTHVGNVLAKLGARDRTQAVVAAYESGFIDPRRPVD
                     V"
     gene            complement(7152..8408)
                     /locus_tag="VFE39_10885"
     CDS             complement(7152..8408)
                     /locus_tag="VFE39_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6259146.1"
                     /translation="MSRPLRLLVAPLVSGLTYRRFVHLLLGAVLLLPYLGLGALFVAS
                     VNEGGLDPFGVMLVLVAATAAAVGVALVPGVRALEITAARALLGAPVPDPEPARSDAW
                     PARRRAAGWLLLNLVAGGLTALVTLAVVPASIGFLIAPWRELTPFPTGVRAAWLPVVG
                     LLLPLLLLHAVSAAGAVLGRLAVRVLGPSPAERMAVELTRAREAERALAERNRLAREL
                     HDSVGHALTVTTLQAGAAARVGTDPTFVAGALDAIAEAGRSALEDLDHVLGLLRDGPD
                     GAAEPDRAPVPDLGDLEALLAGARSAGVTLDVEVRGPTDAVPRVVSREAYRIVQEGLT
                     NALRHAGPVPVAVRVTVVGPDVLELELTNPLGAHGSSRTGGGRGLSGMRERVHVLRGD
                     LVAGSGGRVWQVLARLPLRGSEMMTS"
     gene            complement(8618..9358)
                     /locus_tag="VFE39_10890"
     CDS             complement(8618..9358)
                     /locus_tag="VFE39_10890"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HET6259147.1"
                     /translation="MSTTEELFATDAYRRSFEASITEVDREAGRVALARTAFYPGGGG
                     QPHDLGTLDWGAGPVAVARVKRDAGRIWHWLDADELPGAGTEVAGELDWSRRHLLMRT
                     HTALHVLCGVVWSEFAIPVTGGNMEPGAGRLDFPLESISAELGRKLEARINEELTAAR
                     AVVVEFLGRDAADADPALIRTAANLIPREIDPLRVIAIVGLDKQADGGTHVASTAEVG
                     AVRVTGTESKGKGNKRVRIEVMEAPATG"
     gene            complement(9390..9761)
                     /gene="hisI"
                     /locus_tag="VFE39_10895"
     CDS             complement(9390..9761)
                     /gene="hisI"
                     /locus_tag="VFE39_10895"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HET6259148.1"
                     /translation="MTARVQQSALDPAIAARLKRTPDGLVCAIAQQHGTGEVLMVAWM
                     DDEALHRTLTTGRATYWSRSREEYWVKGATSGHTQQVHEVRLDCDGDAVLVVVDQTGA
                     ACHTGTRTCFETDVLLVRDGA"
     gene            complement(9758..10531)
                     /gene="hisF"
                     /locus_tag="VFE39_10900"
     CDS             complement(9758..10531)
                     /gene="hisF"
                     /locus_tag="VFE39_10900"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HET6259149.1"
                     /translation="MSVAVRVIPCLDVDAGRVVKGVRFADLRDAGDPVEMARLYDAEG
                     ADELTFLDVTASSAERATMLDVVRRTAEQVFIPLTVGGGIRSTDDVDTLLRAGADKVS
                     INTAAIARPELLHEASRRFGAQCIVLSVDARTVPEGSEPTPSGWEVTTHGGRRGTAID
                     AVEWAARGQELGVGEILLNSMDADGTRAGFDIGMIAAVRKVVDVPVIASGGAGAVEHF
                     LPAVRAGADAVLAASVFHFGQLRIADVKNALRGGHVEVR"
     gene            complement(10545..11312)
                     /gene="priA"
                     /locus_tag="VFE39_10905"
     CDS             complement(10545..11312)
                     /gene="priA"
                     /locus_tag="VFE39_10905"
                     /EC_number="5.3.1.16"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase/phosphoribosylanthranilate isomerase
                     PriA"
                     /protein_id="HET6259150.1"
                     /translation="MSFTLLPAVDVADGQAVRLVQGEAGTETGYGAPREAALQWQNDG
                     AEWIHLVDLDAAFGRGSNAELLAAVVGELDVAVELSGGIRDDASLERALGTGCARVNL
                     GTAALEDPAWCARAIAAHGDRIAVGLDVRITDAGHRLAARGWTTDGGDLWEVLERLDR
                     DGCARYVVTDVSKDGTLRGPNIDLLRDVAGVAGAPVIASGGIAEVSDLVALAEVAATG
                     VNIEGSIVGKALYAGRFTLPEALAAVRAVDVAGRAAG"
     gene            complement(11469..12728)
                     /locus_tag="VFE39_10910"
     CDS             complement(11469..12728)
                     /locus_tag="VFE39_10910"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HET6259151.1"
                     /translation="MALPPGPELGALLAGLDLAQVPNDHVVDVLRAHARQVAHEQARM
                     LAAIVEVGRSTPFPDPTDAEGRKVLGARRLMAVHEWASGEIAAALTLTSTAADRELSF
                     ADTVVTRLPQVFAALSAGLIDRPKAWVFADHLDDLIAEQIAAICSVLVPMAPRLTTGR
                     LRARLVRMIQEIDPEHARRRYQKAVRERCVVGYLDRDGTATINASGLPVDEAAAACER
                     LELLGETVKRAGHPGRLAQIQTDLFLGMLDGRFHGLTEKQIVTELLARSREDRPCPAP
                     LVPDPADSADSGTDSTGSEASGGDTAGRGTCCTDASGGEAWGAAGLPASPTRTTRTTT
                     PEAGPPPAATSVPGATATTSTSTRAAGAWASPSQGTSSGPARSDAPTARGALRSRLRS
                     WRRVRRRRIRAGLPTSRPAASPRMVRS"
     gene            complement(12813..13448)
                     /gene="hisH"
                     /locus_tag="VFE39_10915"
     CDS             complement(12813..13448)
                     /gene="hisH"
                     /locus_tag="VFE39_10915"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HET6259152.1"
                     /translation="MSQIVVLDYGSGNLRSAERALRRVGADVTVTADMRAALEADGLV
                     VPGVGAFAACMAGLSAVKGPQIIDRRLAGGRPVLGICVGMQVLFDLGVEWGERTAGCG
                     QWPGTVERVKADVLPHMGWNTLRVPAGSTLFAGLDADTRFYFVHSFGVRRWEMEESDV
                     LKPPLVTWAHHGEDFVAAVENGPLAATQFHPEKSGDAGAAVLQNWLRDVVG"
     gene            13609..13731
                     /locus_tag="VFE39_10920"
     CDS             13609..13731
                     /locus_tag="VFE39_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259153.1"
                     /translation="MSLGAFEAIAVATAMPTVAVALDGLAVYALAFAEPRWPEG"
     gene            14028..14351
                     /locus_tag="VFE39_10925"
     CDS             14028..14351
                     /locus_tag="VFE39_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259154.1"
                     /translation="MQAAVAHPGEPLAPLSGLALAGGLALFYLGELAYRWRDHHRLMV
                     DRLVAGAAAAAVIPIADRVPAVATLGVLLLIGALRVAWEIRSGVFPRPAGRPVRGRPS
                     WTRSR"
     gene            complement(14317..14916)
                     /gene="hisB"
                     /locus_tag="VFE39_10930"
     CDS             complement(14317..14916)
                     /gene="hisB"
                     /locus_tag="VFE39_10930"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HET6259155.1"
                     /translation="MSRIGRVERITKESKVLVEIDLDGTGTTEIATGVPFFDHMLDAL
                     GKHGAFDLTVTASGDVDIDVHHTVEDVAIVLGQAIRQALGDKKGIRRFGDAWIPMDEA
                     LAQAVVDVSGRPYTVHTGEPDVMQGFVVGGHYPTVLNRHVFESLAFHGHLALHIRVLD
                     GRDPHHITEAEFKAVARALRAAVEPDTRITGIASTKGAL"
     gene            complement(14943..16070)
                     /locus_tag="VFE39_10935"
     CDS             complement(14943..16070)
                     /locus_tag="VFE39_10935"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HET6259156.1"
                     /translation="MTALIPGVGAEVSLDDLPLRDDLRGRSPYGAPQLDVAVRLNTNE
                     NPYPPPPELVADVIEAARAAAAELHRYPDRDAVALRTDLAEYLSGATGVPLGPANVWA
                     ANGSNEVLQQIMQAFGGPGRTAVGFEPSYSMHPIIAAGTRTEWLPAPRRADFTIDVDA
                     AAAVLRERAPDLTFLTSPNNPTGQSLEPADLATLIDAAPGLVVVDEAYAEFSDRPSAI
                     TLLGSRGAKLIVSRTMSKAFAFAGGRLGYLAAAPAVVDALQLVRLPYHLSTLTQAAAR
                     AALRHADATLGTVALLRAERDRVVADLAATGYDVVPSDANFVLFGSFAEAGRAWRAFL
                     ERGVLVRDVGIPARLRVTIGTPEENDAFLHVAAEVAAAQVV"
     gene            complement(16067..>16339)
                     /locus_tag="VFE39_10940"
     CDS             complement(16067..>16339)
                     /locus_tag="VFE39_10940"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HET6259157.1"
                     /translation="RTVRNAGAIFVGPFAPVSLGDYCAGSNHVLPTGGCARHSAGLSV
                     QTFLRGVHIVEYTEDALRDVADQVVTLAAAEDLPAHGQAVTARFSR"
BASE COUNT         2277 a         6094 c         5767 g         2201 t
ORIGIN      
        1 gcccgccatt ccagatcttc agggcgccga ccgggtcgcc accggggccg aagtaggttt
       61 cccagtcggt ggccacgtgg taaagacggc cgcccaccag cccgaacggg accgcgaaca
      121 cagccacgtc cgtgaccgtg ccgggctggc cgccgcgcgc gacgaagcgc cgctcgctcc
      181 acatcaccgc gacgatgatg ccgacgatga tgcagatcgc gtacgcccgg atcgggatcg
      241 ggccgagcat ccagaccccg cgatccgggc tcggaatgaa ggccagcagg gctgtcgcca
      301 cggcagaact caccacgtca cggtatcgcc atgcctcggc agcaggtcgg agcggtcgtg
      361 gtggctcatg gtgtggtcag gccggggcgg gtgtggcgga tggccgaccg gcatgtcgga
      421 caccgcccgc gagctccgct gccagtgcac ggacatcccc ggcgccaccc cgggcggccg
      481 ccgtgacgaa cgccgacccg acgatgacgc cgtcggcgaa ggaggcgacc tcggccgcct
      541 ggtccgccga ccgcaccccg agcccgacgc cgaccggcag cgtcgtgtgt gcacggcacc
      601 gcgcgacgag ctccggggcg accgaggcga cgctgtcccg cgctccggtg acgcccatcg
      661 tcgaggtggc gtagaggaag ccgctgcccg ccgccgcggt ggaggcgatc cgctcctcgc
      721 tggaggacgg cgcgacgagg aagatccggt ccaacccgtg cgcggtggag gcggcgaacc
      781 actcgtcggc ctcgtccggg atcaggtccg gggtgatcat gccgagcccc ccggcggcgg
      841 cgaggtcgcg cgcgaacgcg tcgacgccgt agcggagcac cgggttgtag taggtcatga
      901 tgacggcccg cccgcccgcc gccgtgaccc gctcgaccac cgacagcacg tcacgcagct
      961 tcgtgccggc ccgcagcgcc gtctccgccg cggcctggat ggtcgggccg tccatcaccg
     1021 ggtcggagta cgggatgccg atctcgagca ggtcgcagcc gccctcgacg agcgcaccga
     1081 acagctccac cgagccgtcg acagtcgggt agccggccgg gaggtagccg accagcgttc
     1141 cacggtcctg ttcccgggac tcccggaaga tcgcctcgac cgcactcatc gctcggcctg
     1201 cccatcggga gacgtcactg cgcgcaggcc ctgtgacagc gattcgctcg caagctcgcc
     1261 cattaccgct gcacgccctc tgcctccgtg gtgcccggcg tctccccgct gggccggtgc
     1321 tcgggtgcgg cggcgcccgg acctgcgagc gaggcctcgg cgatcgccgt gccgctcgcg
     1381 tcctcggcgc tctcgccgag cccgatcatg ccgaaccact tcgccgcggt gtcgacgtcc
     1441 ttgtcgccgc gccccgacag gttcaccagc acgatgccgt cgggcccgag ctcgcggccg
     1501 aggcgcacag ctccggcgat cgcgtgggcc gactcgatcg ccgggatgat cccctccgtg
     1561 cggcagagca acgcgaacgc gtccatcgcc tcggcgtcgg tgaccggctc gtactcggcg
     1621 cggccgaggt ctttgagcag tgcgtgctcc gggcccactc cgggatagtc gagccccgcc
     1681 gagatcgagt gcgactccgc ggtctggccg tcctcgtcct gcagcaggta ggacatcgcg
     1741 ccgtgcagcg tgcctggcga gccctccgtg agggtggccc cgtgccgccc ggtctcgacc
     1801 ccgtcgccac cgggttcgaa cccgaccagc cggacgcccg gatcgtcgag gaacgcgtgg
     1861 aagatcccga tcgcgttcga cccgccgccg acgcaggccg cgaccgcgtc ggggagccgc
     1921 cccgtgcgcg ccagtacctg ctcgcgggcc tccagaccga tgatccggtg gaagtcgcgg
     1981 accatcgtcg ggaacgggtg tggtccggca accgtgccga gcaggtagtg cgtccggtcg
     2041 acgttcgtga cccagtcgcg cagcgcctcg ttgatcgcgt ccttcagcgt gcgggaacct
     2101 gtcgtgacgg gcacgacctg cgccccgagc agccgcatgc gcgcgacgtt gagcgcctgc
     2161 cgctcggtgt cgacctcgcc catgtagacc acgcactcca ggccgagcag tgcgcacgcc
     2221 gtggccgtgg cgaccccgtg ctggcccgcg cccgtctcgg cgatgacgcg cgacttgccc
     2281 atccgctggg tgagcagtgc ctgacccagg acgttgttga tcttgtgaga accggtgtgg
     2341 ttgaggtcct cgcgcttgag caggatgcgc gccccgcccg cgtgctcgcc gagcttcggg
     2401 gcgtcggtga gcggggacgg gcgcccggag tagtcccggt gcagccggtc gagctcgtcg
     2461 aggaaatcgc ggtcgccacg ggccttgtcg taggccgcgg ccagctcgtc gagtgccgcg
     2521 atcagcgcct ccgggacgaa ccggccgccg tagcgcccga aatggccgcg ctcgtccggc
     2581 tcgtgcccgg acggcgtcga gatgccgtcg ctggccttgc tggtggtccc cgaaccgctg
     2641 gtcgtcaaca caacatcctt gctacggcat ccttgttagg gcgtcatcct tcttaccgca
     2701 ccatcctgga gcatgacggg tggaagcccg ccgccaccag gctgcgcacc gcgccgccgg
     2761 ggttgccgct ggtgaccaac ccctcgccaa caagcaccgc gtcggcaccc cagcccgcgt
     2821 aggtgagcag gtcgccgggc ccccgcacac ccgactcggc cacccgcagc acgtcgctgg
     2881 gcagcccggg cgcgatctgc ccgaagatcg aacggttgac ctcgagggtg tgcaggttcc
     2941 gcgcgttcac cccgatgacc ttggcccccg cttcgagcgc gcggtcggcc tcctcctcgg
     3001 tgtgcacctc gacgagcgcc gtcatgccga gcgactcgac tcggtccagc agtgagtcga
     3061 gcgcattctg ctccagggcc gcgacgatca gcagcaccag atcggctccg taggcccgtg
     3121 cctcgtgcac ctggtacggg ctgacgacga agtccttgcg cagcaccgga acgtccaccg
     3181 cggcgcggac ggcggccagg tcggccagcg aaccgccgaa ccggcggcgc tcggtgagga
     3241 cactgatcac ccgcgccccg ccaccggcgt aggccaccgc gagatcggcc gggtcggcga
     3301 tgaccgccag atcacccttg gacgggctac gccgcttgac ctcggcaatc accccgatgc
     3361 cgggtgcgcg caacgccgcc atcacatcgc ggggcggggc cgtggccgcc gcgcgccgct
     3421 tgatctcggc gaagtcgacc tccgcctcac gtaccgcaag atcggccctg acaccgtcca
     3481 cgatggaatc gaggacgctc gctccgccgt gctccatggc gttggtcccc ctctccggat
     3541 agggccgggc gcgaatcatg ctagccaggc ccggtacggg ctccgtcgcc aggatcgtcc
     3601 cccctgctcc cgggcacgtc ggcggtgggg tccatccccg cgtcgagcgc ctgccacgcg
     3661 gcccggtcgg ggtccacctc ggcccgccgg tcccctggtg ccgcataccg cgacccgaat
     3721 ctcggcagcc gcggttcgcg cagcagcacg aacagcccga ccgcgagcag cgcggcggcc
     3781 cccgcgacgg cgagcagcgg tgccggcgtc cgggcgacgg ggtccggcgc ggtcggcagg
     3841 acgaccagcg cccgcaccgc gaccacgccc gccacgacag ccaccagcgc gagcagcgcc
     3901 ccgacgagcc tgcgcagcac cccgccgatt gccacaaccg ccgccaccgc ggccagtgcg
     3961 agcagcgcca cgccggtgag cgacggcgca acctgcgccc cggtggattc ggtgccgggc
     4021 cgcccggcta cgcccgcgcc gtgataccag acggtggccg aggcggccca cagcagggcg
     4081 gccgaacccg ccagtcctgc gcacacggcg accagcaccc gtgccttccg cgtgcgcgtc
     4141 tgggtcaccg tgggggaccc ggcgtctcgg cggtatcggt gccgatcggc ggctccgcaa
     4201 gcgtcgccgc ggtcgccacc gccgacaaca ccgcccgcgc cttgttcagg ctctcggtgt
     4261 cctctgccgc cgggtcggag tcggcgacga tcccgccgcc ggcctgcacg taggcgatcc
     4321 cgtcgcgcac cacggccgtg cggatcgcga tcgccgtgtc ggcgtcgccc gcgaagtcga
     4381 ggtagccgac gattccgccg tacaggcccc gccgcgtggg ctccagctcc tcgatgatct
     4441 gcaacgcccg cggcttcggt gctcccgaca gggtgcctgc ggggaagcac gcgatcacgg
     4501 cgtcgaacgc gttgcagtcg cggcgcagcg tgccggtgat cgtcgacacc aggtgcatga
     4561 cgtggctgta gcgctcgacg gagaagaagc tgtgcacctt gaccgtgccc gcctcgcaga
     4621 cccggccgag gtcgttgcgg cccaggtcga cgagcatcac gtgctcggcg cgctccttct
     4681 cgtcgctgcg cagctccttc tcgagcagca ggtcctcctc ctcggtggcc ccgcgccacc
     4741 gggtgcccgc gatcggatgc gtggtcgcct tgccatcgcg caccgtgacc agggcctccg
     4801 ggctggaccc gacgatgtcg aagcgccgtg ccgggtgcgt cgacgttccg gcggactcca
     4861 gccgcagcag gtacatgtac gggctcgggt tggtggctcg cagcacgcgg tacacgtcga
     4921 gcgggtcggc cgggcattcc gcctcgaacc gctgcgagac gaccacctgg aacgcctcgc
     4981 cgtcccggat ctgctccttc acgacctcga cggcggcgtg gtactcggcg ggggaacggc
     5041 gccgggtgaa cctcggctcg acgggctggt acaccgccac cgtggacgcg gcaggtgccg
     5101 cgagctcggt cgtcatccgg tcgagccggg cgacggcgtc gtcgtaggcc tcgtcgacgc
     5161 gctcgtcgcg cgcgtcccag ttgatggcgt tggcgatcag cgtgatcgtg ccctcgtggt
     5221 ggtcgaccgc agccaggtcg gcggccagca gcatcatcag ctcgggcagt tggaggtcgt
     5281 cggtggtgag ctcgggcagc cgctccaggc gccgcaccac gtcgtagccg aggtagccga
     5341 ccatcccacc ggtcagcggc ggcagcccgg ggagcggctc actgcgcagc tcctcgacga
     5401 cggtccgcaa cgccgcgagc gggtcacccg acgtcggcag cccgaccggc acttcgccgg
     5461 tccagaccag ctcgccgtcg acggacgtca gcgcggccgc gctgcgcgct ccgacgaagg
     5521 accagcgcga ccaggaccga ccgttctccg ccgactcgaa caggaacgtt ccggggcgat
     5581 cacctgccag cttgcggtag accccgaccg gggtctcgtc gtcggcgagc agcctgcggg
     5641 tcaccgggat cacccggtgc ccgacggcca acgcgcggaa ttcttcacgg ctgggggtga
     5701 ccgcgccgag ggcgggcgca ggcgcggtga cggtcatgcc gacattgtgg caacacgcgc
     5761 gcaaccaagc acgagagctg tcccaccatc cgcccgcgag aggagctcgg aggaggatgg
     5821 agccgtggcc gcccccgagg agcactcccc gcccggcgcc cgtggagcgc ccgtcgacgc
     5881 cgggtcagcc gacgccgggc cccgccgccg cgggcgcaga ccgggcggcg ccgacacccg
     5941 tgcccagctg ctcgccgccg cccgtgtgga gttcgccgac cgcggctacg acggcgcgac
     6001 ggtgcgggtg atcgccgagc gggcgggcgt ggaccctgcg atggtcaacc actggttcgg
     6061 tggcaaggag tcgctgttca ccgcggcgct ggacctcccg gtggaccccg gtgtgattct
     6121 cgccgacgtg gtgcccggcg atcccgacca tctcggcgag cggatcatcg cgcggttcct
     6181 ggagatctgg gacaccacag gcggggcgcc cctcgccgcc gtgctccaga gcgtggcagg
     6241 ccacgacgcc gcggcccgga tgctgcggga tttcgtgagc cgcgtcctcg tcgggaaggt
     6301 ggtgtcgacg gtggcaccgg accggccgga gctgcgcgcg gggctgtgcg gctcccaggt
     6361 gttcggcctg gcgatcatcc ggtacgtcct caaggtggag ccgctcgcgt cggccgacca
     6421 cgccaccgtg atcggcgcag tggcgccgaa cctgcagcgc tacctcaccg gtccactgcc
     6481 gtgagagaga ccaactcaga catcgacggg tcggcggggg tcgatgaacc cggactcgta
     6541 ggccgccacc acggcctggg tgcggtcgcg ggcaccgagc ttggccagca cgttgccgac
     6601 gtgggtcttc acggtctcca caccgaccat cagcgccctg gcgatctcgg cgttcgacag
     6661 gccacgagcc acccagcgca gcacctcgcc ctcccgctcg gtaagcccgg cgctgcgtag
     6721 cgcatccccg cccatacggc ccctggtggc cacgagatcc cgcaccgccg cggggaacag
     6781 cagcgaatcg ccgccggcga ccgtacgtac cgcctgcacg atctcggcgg gccgcgcgcg
     6841 cttgagcagg aacccggtgg cgcccgcctg cagcgcgtcg tacacgtgtc cgtcgttgcc
     6901 gaacgtcgtg agcacgagca cccggggcgg gttcggcagg gtcgtgacca ggtgccgggt
     6961 tgcggcgatg ccgtcgactg cctgcatccg cacgtccatc agcaccacgt cgggcgcgaa
     7021 cctgcgtacc agcccgggaa catcggcgcc gtcggaggcc tccccgacga ccgtgatgtc
     7081 cggctcggcg ccgaggatcg tgcggagccc ggcccgcacg agctcctcgt cgtccaccag
     7141 caacaacttg atcatgacgt catcatctcg gatcccctca acgggagccg ggccagcacc
     7201 tgccacactc tgccaccgga tccggccacc aggtcaccgc gcagcacgtg cacgcgctcc
     7261 cgcatcccgg acagcccgcg cccgccgccg gtacggctgc tgccgtgggc gccgagcggg
     7321 ttggtcagct ccagttcgag cacgtcgggg cccaccaccg tgacccggac cgcaaccggc
     7381 accggtcccg cgtggcggag ggcgttggtg aggccctcct gcacgatccg gtaggcctcg
     7441 cgggacacca cccggggcac cgcgtccgtc ggcccccgca cctccacgtc cagcgtgacc
     7501 cctgccgatc gggcgccggc caggagcgcc tcgaggtcgc ccaggtcggg caccggcgcc
     7561 cggtccggtt cggcggcgcc atccggcccg tcgcgcagca gcccgagaac gtggtccaga
     7621 tcctccagcg cgctccggcc cgcctcggcg atcgcgtcga gcgctccggc gacgaacgtg
     7681 ggatcggtgc cgacccgggc ggccgcaccg gcctgcaggg tggtgacggt cagcgcgtgt
     7741 cctaccgagt cgtgcagctc cctggcgagc cggttgcgct ccgccagtgc ccgctccgcc
     7801 tcccgcgcac gggtgagctc gacggccatc cgctcggcgg gcgagggacc gagcacgcgc
     7861 accgcgagcc ggccgagcac cgccccggcg gccgagaccg catgcagcag gagcagcggc
     7921 agcagcagcc ccaccaccgg aagccacgcc gcgcggacac cggtgggaaa cggggtgagc
     7981 tcacgccacg gcgcgatcag gaagccgatc gacgccggca cgacggccag tgtcacgagc
     8041 gctgtgaggc cgcctgccac caggttcagc agcagccatc cggccgcgcg gcggcgcgca
     8101 ggccacgcgt ccgacctcgc gggttccggg tccggcaccg gggcgccgag gagggcacga
     8161 gctgcggtga tctccagggc ccgcacgccc ggcaccagcg ccacccctac cgccgctgca
     8221 gtggccgcca cgagcaccag catcacgccg aacgggtcca gcccgccctc gttgaccgac
     8281 gcgacgaaca gcgccccgag ccccaggtac ggcagcagca gcaccgcgcc cagcagcaga
     8341 tgcacgaacc ggcggtaggt cagccccgag acgagcgggg ccaccagcag ccgtagcggg
     8401 cgggacacgg gacgatcgtc gcagaagcgg gtaccggaga tcctcccccg cgcgagggag
     8461 cccgatcgtt tcgcggggag tcaccgggcc caatggggcg gcgctgttcc ccggcatgac
     8521 agattgcgca gctcacaggt ctgttcggcg gcttcgccct cgcttcgtgg cgggctgctg
     8581 gccctcaccg gtaccggcgt ggcccatccg gccgccgtca gcccgtcgcg ggtgcttcca
     8641 tgacctcgat ccgcaccctc ttgttcccct tgcccttgga ctcggtgccg gtgacccgca
     8701 cggcgccgac ctcggccgtg ctcgcgacat gcgtgccgcc gtcagcctgc ttgtcgaggc
     8761 cgacgatggc gatgacgcgc agcgggtcga tctcgcgcgg gatgaggttc gccgccgtac
     8821 ggatcagcgc ggggtcggcg tcggccgcgt cacggccgag gaactcgacg accaccgcac
     8881 gggcggccgt cagctcctcg ttgatccgcg cctccagctt gcggcccagc tcggccgaga
     8941 tcgactccag cgggaagtcc agcctccccg cgcccggctc catgttgccg ccggtgaccg
     9001 ggatggcgaa ctcgctccac accaccccgc acaacacgtg cagcgcggtg tgcgtgcgca
     9061 tcagcaggtg gcggcgactc cagtccagct cgcccgccac ctcggtgccc gcgcccggca
     9121 gctcgtcggc gtcgagccag tgccagatgc ggccggcgtc ccgcttcacc cgggcgacgg
     9181 cgaccggtcc tgcaccccag tccagcgttc ccaggtcgtg cggctgcccg cctcctcccg
     9241 ggtagaacgc ggtgcgcgcc agcgccaccc gaccggcctc ccggtcgacc tccgtgatgc
     9301 tcgcctcgaa ggaccggcga taggcgtcgg tggcgaagag ctcctcggtg gtgctcatgc
     9361 gccgtccctg acaagcagga cgtcggtgct catgcgccgt ccctgacaag caggacgtcg
     9421 gtctcgaagc acgtccgcgt ccccgtatgg cacgccgcac cagtctggtc gaccaccacg
     9481 agcacggcgt cgccgtcgca gtcgagacgc acctcgtgca cctgctgggt gtggccggag
     9541 gtggcgccct tcacccagta ctcctcgcgg gagcgcgacc agtaggtggc ccggccggtg
     9601 gtgagggtgc ggtgcagcgc ctcgtcgtcc atccacgcca ccatcagcac ctcgccggtg
     9661 ccgtgctgct gggcgatcgc acagaccagc ccgtctggag tgcgcttgag ccgggccgcg
     9721 atcgccgggt caagggcgga ctgctgcaca cgcgccgtca tcggacctcc acgtgcccgc
     9781 cccgcagggc gttcttgacg tcggcgatgc gcagctgccc gaagtggaag acgctggcgg
     9841 cgagcaccgc gtcggccccc gcccgcacgg cgggcaggaa gtgctcgacg gcgcccgcgc
     9901 ccccgctcgc gatcacgggg acgtccacga ccttgcgcac cgcggcgatc atgccgatgt
     9961 cgaagcccgc ccgggtgccg tcagcgtcca tcgagttgag caggatctcc cccacaccga
    10021 gctcctggcc gcgcgcggcc cactcgacgg cgtcgatcgc ggtgccccgg cgcccgccgt
    10081 gggtggtgac ctcccagccc gacggggtcg gttcgctccc ctcgggcact gtgcgggcgt
    10141 cgacggacag cacgatgcac tgcgccccga accggcggct ggcctcgtgc agcagttccg
    10201 ggcgcgcgat cgccgccgtg ttgatgctga ccttgtcggc cccggcccgc agcaacgtgt
    10261 cgacgtcgtc ggtgctgcgg atcccgccgc cgaccgtgag cgggatgaat acctgctccg
    10321 ccgtgcggcg caccacgtcc agcatcgtgg cgcgctcggc agacgacgcg gtgacatcga
    10381 ggaacgtgag ctcgtcggcg ccctcggcgt cgtagagacg ggccatctcg accgggtcgc
    10441 cggcatcgcg gaggtcggcg aaccggaccc ccttcaccac ccggcccgcg tcgacgtcga
    10501 ggcacgggat gacgcgcacg gccacgctca tgaggatccc cctgtcatcc cgcagcccgg
    10561 ccggcgacat cgacggcccg gaccgcggcc agggcctcgg gcagggtgaa ccggccggcg
    10621 tagagcgcct tgccgacgat cgagccctcg atgttcactc cggtcgccgc cacttcggcc
    10681 agcgccacga ggtcggacac ctcagcgatg ccacccgacg cgatgaccgg cgcgccggca
    10741 accccggcga cgtcacgcag caggtcgatg ttggggccgc gcagggtgcc gtccttgctg
    10801 acgtcggtga cgacgtagcg ggcgcatccg tcgcggtcga gccgctccag cacctcccac
    10861 aggtcaccac cgtcggtggt ccagccacgg gcggcgagcc ggtggccggc atcggtgatc
    10921 cgtacgtcga gcccgaccgc gatccggtcg ccgtgagcgg cgatggcgcg ggcgcaccac
    10981 gcggggtcct ccagcgccgc ggtaccgagg ttcacccgcg cgcagccggt gcccagcgcc
    11041 cgctccagcg acgcatcgtc gcggatgccg ccggacagct ccaccgcgac gtcgagctcc
    11101 ccgacgacgg cggccaagag ttcggcattg gagccacggc cgaacgcggc gtccagatcg
    11161 acgaggtgga tccactcggc gccgtcgttc tgccactgca gcgcggcctc ccgcggcgcg
    11221 ccgtaaccgg tctcggtgcc ggcctcgccc tggaccaggc ggacggcctg accgtcggcg
    11281 acgtcgacgg cgggaagcag cgtgaaactc acaccagaag cgtaccgagc gggcacatcc
    11341 gacctcgccg gcgttgcgat atcagtaccg atcccgaggc ggtcggcgtc agaagggcgg
    11401 caggtcgtcg aggtcgaccg gctgcggtgg ttccggccgc ggcggcgggc gggccggttc
    11461 cggtcgggtc aagatcgaac cattctcggc gaagcggccg gacggctcgt cggaaggccc
    11521 gcccggatcc gacggcgccg gacacggcgc cacgaccgga ggcgagatcg gagcgccccg
    11581 cgtgcggtag gcgcgtccga gcgggctggt ccagacgaag tgccctggct cgggctggcc
    11641 caggcaccag ccgccctcgt gcttgtagag gtggtggcgg tcgcacccgg gaccgatgtt
    11701 gcggctggtg gtggccccgc ctcgggcgtg gtcgtgcgtg tggtccttgt cggacttgcg
    11761 ggcaggccgg cggcacccca cgcctcgccc cctgacgcgt cggtgcagca cgtgccgcga
    11821 ccagccgtgt cgccgccgga cgcctcgctc ccggtcgagt cggtaccgct gtcggctgag
    11881 tcggccggat cgggtacgag cggcgccggg caggggcgat cctcccggga ccgggcgagc
    11941 agctccgtga cgatctgctt ctccgtcagg ccgtggaagc gaccgtcgag catcccgagg
    12001 aacaaatccg tctggatctg agcaagccgg ccggggtgtc cggcccgctt gaccgtctcc
    12061 cccagcagtt cgaggcgttc gcacgccgca gccgcctcgt cgacgggcag gcccgaggca
    12121 ttgatcgtcg ctgttccgtc gcggtcgagg tagccgacga cgcaccgctc ccgcacggcc
    12181 ttctggtacc gccggcgcgc gtgctccgga tcgatctcct ggatcatccg cacgagccgg
    12241 gcccgcagcc ggccggtggt gaggcgcggc gccatcggca ccaggaccga gcagatcgcg
    12301 gcgatctgtt cggcgatgag atcgtccagg tgatcagcga agacccacgc ctttggccgg
    12361 tcgatcagtc cggccgagag cgccgcgaac acctgaggca gccgcgtgac gaccgtgtcg
    12421 gcgaaggaga gctcgcggtc ggccgctgtg ctggtgagcg tgagcgctgc ggcgatctcc
    12481 cccgaggccc attcgtgcac cgccatgagc ctccgcgcac ccaggacctt gcgcccctct
    12541 gcgtcggtgg ggtcggggaa cggtgtggat cgaccgacct cgacgattgc ggccagcatc
    12601 cgcgcctgct cgtgcgccac ctgtcgggca tgcgcgcgca gcacgtcaac gacgtggtcg
    12661 ttgggaacct gggcgagatc gagccctgcc agtagcgcgc cgagctccgg ccctggcggc
    12721 agtgccacca gaccaccgga aacgcgacca ccatcctgca cgagtcgaac atacattcga
    12781 cccctgacag tttgtcgatc aggcgactcg gctcagccga cgacgtcgcg cagccagttc
    12841 tgcagcacgg cggcgccggc gtcgccggac ttctccggat ggaactgggt ggccgcgagg
    12901 gggccgttct ccaccgccgc gacgaagtcc tcgccgtggt gcgcccaggt gacgagcggc
    12961 ggcttcagca cgtccgactc ctccatctcc cagcgccgca ccccgaagga gtgcacgaag
    13021 tagaagcggg tgtcggcgtc gaggcccgcg aacagggtgg agccggcggg gacgcgcaag
    13081 gtgttccagc ccatgtgcgg cagcacgtcg gccttgaccc gttcgacggt accgggccac
    13141 tgcccgcaac cggcggtgcg ctccccccac tccacgccga ggtcgaagag cacctgcatc
    13201 cccacgcaga tgcccagcac cggacggccg cccgcgaggc ggcggtcgat gatctgggga
    13261 cccttgacgg cgctgaggcc ggccatgcac gcggcgaagg cgcccacccc ggggacgacg
    13321 aggccgtcgg cttcgagggc ggcccgcatg tcggccgtga ccgtgacgtc ggcgccgacg
    13381 cggcgcaacg cgcgttccgc ggaacgcagg ttgcccgatc cgtagtccaa cacaacgatc
    13441 tgtgacacgc ggcccaggat agccgcaact cagttgacct cgaccccggt tgaagttgca
    13501 cggtgccggc cgtgaccacg accgcgcgcc cgaggaaatt cgcccggtgc cgcccgcggt
    13561 ctgataggcg gacccctgcg ggcgctgacc gtcggctccg tcgctctcgt gtccctcggc
    13621 gcgttcgagg cgatcgccgt cgcgacggca atgccgaccg tcgccgtcgc cctggacggc
    13681 ctcgccgtgt atgccctcgc gttcgcggaa ccacggtggc cggagggctg acgctggtgg
    13741 cggtgctcgt cgcaggacgg gcccgcgtag gaacggtatg accccgccac ggccgagtgc
    13801 ggtggccaca gacgttgccg gggcgcgagg acccggtgat tcgtggccgt cgcactagac
    13861 taggggaggg gtccagcagg cagtcgacct cccggccctg gatgccggcg tggtgccggc
    13921 aggtccagtg ggcaggtgcc gcctggtggt ccgaggtcca ggccgagcga ccgggggtgc
    13981 ctgaactctg acgctcgcgc gcctggccgt gccacctacc aatcggcgtg caggcggcgg
    14041 tcgcccaccc cggggagccg ctggcgccgc tgtcgggcct cgccctggcc ggcggcctcg
    14101 cgctgttcta cctcggcgag ctcgcctacc ggtggcgcga ccaccaccgg ctgatggtgg
    14161 accggctcgt cgccggggcg gccgcggccg ccgtcatccc gatcgcggac cgggtccccg
    14221 cggtggcgac gctgggtgtc ctgctgctga tcggcgccct gcgtgtcgcg tgggagatcc
    14281 ggtcgggggt cttcccgagg ccggccggcc ggcccgtcag agggcgccct tcgtggacgc
    14341 gatcccggtg atccgcgtgt ccggctccac ggcggcgcgc agtgcacgtg cgacggcctt
    14401 gaactcggcc tccgtgatgt ggtgcgggtc gcgcccgtcg agcacgcgga tgtgcagcgc
    14461 gaggtggccg tggaaggcca gcgactcgaa gacgtgccgg ttgagcaccg tcgggtagtg
    14521 cccgccgacc acgaacccct gcatgacatc cggctcaccg gtgtgcacgg tgtagggccg
    14581 cccggaaacg tcgacgacgg cctgggcgag ggcctcgtcc atcgggatcc acgcatcgcc
    14641 gaagcggcga atgcccttct tgtcgcccag ggcctgccgg atcgcctggc cgagcacgat
    14701 cgcgacgtcc tcgacggtgt gatgtacgtc gatgtccacg tcgcccgacg ccgtgacggt
    14761 caggtcgaac gccccgtgct tgcccagcgc gtcgagcatg tggtcgaaga acgggacccc
    14821 ggtggcgatc tccgtggtgc ccgtcccgtc gaggtcgatc tcgacgagga ccttcgactc
    14881 cttcgtgatc cgctccacgc gaccgatccg gctcactcct gaaacctccg tcactgcctg
    14941 cgtcacacaa cctgcgcggc agcgacctcc gctgccacgt gcaggaatgc gtcgttctcc
    15001 tccggcgtgc cgatcgtgac cctcaaccgc gcagggatcc cgacgtcacg aaccagcaca
    15061 ccacgctcca ggaaggcacg ccaggcccgt ccggcttcgg cgaaggagcc gaacaacaca
    15121 aagttggcat cgctcggcac gacgtcgtag cccgtggcgg cgaggtcggc caccacccgg
    15181 tcgcgctcgg cgcgcagcag cgcgaccgtg ccgagggtgg cgtcggcgtg ccgcagcgcc
    15241 gcccgcgccg ctgcctgggt gagcgtcgag aggtggtacg gcaaccggac gagctgcaac
    15301 gcgtcgacga cggcaggcgc cgccgcgagg taccccaacc gccccccggc gaacgcgaac
    15361 gccttgctca tcgttcggct gacgatcagc ttcgcaccgc ggctgccgag cagggtgatc
    15421 gcgctgggcc ggtcggagaa ctcggcgtag gcctcgtcga ccaccaccag gccgggcgcc
    15481 gcatcgatca gcgtggccag gtcggcgggc tccagtgact ggccggtggg gttgttcggg
    15541 ctggtcagga acgtgaggtc gggcgcccgc tcgcgcagga cggcggcagc cgcgtcgacg
    15601 tcgatcgtga agtcggcacg acgtggcgcg ggcagccact ccgtgcgggt gccggcggcg
    15661 atgatcgggt gcatcgagta cgacggctcg aaccccacag ccgtgcggcc cggtccgccg
    15721 aaggcctgca tgatctgctg cagcacctcg ttggaaccgt tggcggccca gacgttggcc
    15781 gggccgagcg gcacaccggt ggcaccgctg aggtactcgg cgaggtccgt gcgcagtgcg
    15841 actgcgtccc ggtccgggta gcggtgcagc tcggcggctg ccgcacgcgc cgcctcgatc
    15901 acgtccgcta ccagctccgg cggcggcggg tacgggttct cgttcgtgtt gagccgcacc
    15961 gccacgtcga gctggggggc gccataaggg ctccggccgc gcaggtcgtc gcgcagcggc
    16021 aggtcgtcga gcgagacctc ggcaccgacc cccggaatga gcgcggtcac cgggagaacc
    16081 gggccgtgac agcctggccg tgcgcgggca ggtcctcggc ggcggcgagc gtgacgacct
    16141 ggtcggcaac gtcgcgcagc gcgtcctcgg tgtactcgac gatgtgcacg ccgcgcagga
    16201 acgtctgcac cgacaggccc gcggagtgcc gcgcgcaccc gccggtgggc agcacgtggt
    16261 tggacccggc gcagtagtca cccagcgaca ccggcgcgaa cgggccgacg aagatcgcgc
    16321 ccgcgttgcg gaccgtgcg
//
LOCUS       DASRSC010000222        14778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3195873,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000222 DASRSC010000000
VERSION     DASRSC010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 14778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14778
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3195873"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1092
                     /locus_tag="VFE39_10945"
     CDS             <1..1092
                     /locus_tag="VFE39_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6259158.1"
                     /translation="ALPRLDIDPAGSATVPTDEPPASAPALIVYTSGTTGSPKGAMLS
                     RGALASNLDALADAWAWTVDDLLVHALPLFHVHGLVLGVIGPLRRGGTLHHIGALDAR
                     TLAGSGASMVFGVPTQYHRLADQLETDPDAAAAIGRARLLVSGSAALTAVDHARLHRA
                     TGLAVRERYGLTETLILTAARAEDEPEPGTVGRPLRGTEVRLTPLAGEQADDLGAVEV
                     RGPGLFDGYLNRPDATAAAHTPDGWFCTGDIGRWTASGALALVGRSATDLIKSGGYKI
                     GAGEIENALLEHAGVAEVAVVGVPDDDLGERIVAFVVPTGIAPPERELVDHVAALLAP
                     HKRPREVRFTDALPRNDMGKVVKARLPRD"
     gene            1167..2702
                     /locus_tag="VFE39_10950"
     CDS             1167..2702
                     /locus_tag="VFE39_10950"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH and CHAD domain-containing protein"
                     /protein_id="HET6259159.1"
                     /translation="MTDTTTRTGNQTYRGPAGTGAPRLAALPGVHRADVTPQQVIEIE
                     RYDTDDHRLTAAGITVALHRRADEPPQWHLDLPDGDVPEHLRVPLADAAGLSPAVPGE
                     IDALVRGAARERAVRPVGRIRRVRTETRLLDEDERLLALVVHDEVTLATLGRATDVEA
                     WTEVELRRAEADNALVAELEERFAESGLRPAVLAAEEELERLLRPTPKARRLRAGKRG
                     SAGAGVMEYLGAQLDRLAAEDLRVRRDEPDAVHQLRVASRRLRSALQAYRPLLDRTRT
                     DPLVDGLRELGRELAPARDAEVLHEHISSGLTGLAPELLLGPVQAQMTRHFAREQAEA
                     RAAVLAVLDSERHMGLRAALDALVERPPLTKRAARPARKEVPALVARTARRLERAVQV
                     ATDPAVPDSQRDAAVHSARKAGKRLRYATEVAKPAVGKQAKKFAKALKGFQSALGEHQ
                     DTVVARGALRELGAMANSAGENGFSFGVLYGQDAARAEQIERDLPVLWSEAWTRRNRR
                     WLR"
     gene            2959..3981
                     /locus_tag="VFE39_10955"
     CDS             2959..3981
                     /locus_tag="VFE39_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET6259160.1"
                     /translation="MSEPRDAVADLRRIAFLLERAHESTYRVRAFRTAAAVLGRRDAD
                     EIAGLVRSGELVRLKGVGEVTARCVAESVAGEEPVYLRRLEATEGTPLDEAAEVLRAA
                     IRGDCHSHTDASDGGSPLREMVQTAQELGHDYLVVTDHSPRLTVANGLSADRLRAQIE
                     QVAELNVELGDGFRVLTGIEVDINEDGSLDQDEDLLAELDVVVASVHSKLRMPRADMT
                     QRMLTAVANPHVDVLGHCTGRMVTGNRKRPESQFDADGVFAACAEYGVAVEVNSRPER
                     LDPPKRLLRLAVEAGCSFTIDTDAHAPGQLDWLGNGCERAAACGVPMERVLNTWTAER
                     LLARSR"
     gene            4403..6157
                     /locus_tag="VFE39_10960"
     CDS             4403..6157
                     /locus_tag="VFE39_10960"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET6259161.1"
                     /translation="MPAAETPSLIPSAAADRARQSDPDSTDRGADSTEPADDQQADGK
                     DSGAGSGDAGATGVSGDSNGGNSANTVNPSVSPDPADPAEPADPAEPADPAEPADPAE
                     PADPAEPADAADPAEPAGPAGPAGPAGPAEPADATERPDPGEPTDTADPADAADPADS
                     ARTASAAGPANQGGPASPGGPASPGGPASPAGSVSPAGPASPAGSRDPAGSVSPVSPV
                     RPVRLVRPVCPAGPPSPAGSGDPAGAGSPAGSGDPAGSGSLGGPASPAGPASPADPAS
                     PPGPTDPADPAGPTNAGNSAAGVPATTQEPADPGERAPADGPAAGEQPGNAALPADSS
                     ECATTAAERYGWGQPARRSDFQDGLPPDWHPYGPEPGHARRGTRTPEAISVANGVATI
                     TATPDGTTGAMSWHPGQRYGRWEACVRADEGQGGINALMLLWPVAEDFPVGGEIDWME
                     IMSDDRQETSFFLHYGENNDQTTGSVRHHSMEWSAYALEWTSEKMTAYVNGDEWYSTT
                     ETDKFPPRPMNMTMQLDYFGGAGGGTAMHMDWAQQWALPESEPAELSLAPGDPATGQP
                     DEYPERAPRVLDGSGATE"
     gene            complement(6252..7187)
                     /locus_tag="VFE39_10965"
     CDS             complement(6252..7187)
                     /locus_tag="VFE39_10965"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HET6259162.1"
                     /translation="MAGEALVDLVPAPVGGYFEMAPGGSPANVAVALSRLGVPARLLA
                     RIADDMLGRRIREHLTVNGVDLDHAVRAREQTSMAMVAVDTDGGPSYDFRIAGTADWQ
                     WTPDELTGALDGPVVALHSGSLALTTPPGAAVLRELITRSAATATISYDPNCRPLLMG
                     DPSDVLAGAHELLAVADVVKVSSEDLEWLVPGSTPDAVLEDWLARGPAVIAITLGGDG
                     ALAGTAAGVRSRRAGIPVPVVDTVGAGDTFSAALLAGLHRRGLLGAAARPELKAIDAD
                     TLDGLLDEAVTAAAITCSRRGADPPTTDDLLARRA"
     gene            complement(7263..8351)
                     /locus_tag="VFE39_10970"
     CDS             complement(7263..8351)
                     /locus_tag="VFE39_10970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6259163.1"
                     /translation="MCANGTPPVTLRMLADQLGLHVSTVSRALHGTSDESGRAASPAT
                     VERIRRLATELDYRPNPHAASLRTRRSNLIGVLVPRLSDIVLAMIYEGIEEAAAEHDL
                     STFVANTRDLPEIQRIRTDMVLARRVDGMIFGDAYVDARFLGEVAARGVPFVLVNRRA
                     GDLPSVTCNDHLGGRLLGEHLLELGHRDVAVVAGEPYASTGIDRTQGFVSAYAEAGIE
                     IPPRRIVRSPFDATGGRAAAEQLLADGPPPSAIFAVNDFAAIGVLGALRDHGLHAGTD
                     IAVAGFNDTPLAAELPVPLTTVRSPMHEMGRRGIELLVRLLRGEQVESEQLRPELVVR
                     VSTTGALRGGGSSGSKQGVARTHVHPPA"
     gene            8499..9377
                     /locus_tag="VFE39_10975"
     CDS             8499..9377
                     /locus_tag="VFE39_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HET6259164.1"
                     /translation="MPKEIFVSIGVDVDAVAGWLGSYGGEDSPCDISRGLFAGEVGVP
                     RLLRLFERKNLRTTWFVPGHSLETFPDQTRMIVDAGHEVGAHGYSHENPIAMTRDQES
                     AILDRSIELIEKLTGNRPTGYVAPWWEFSPVTNELLLERGVKYDHSLMHNDFSPYYVR
                     VGDSWTKIDYSKPAEEWMKPLVRGEESGLVEIPANWYLDDLPPMMFVKASANSHGFVN
                     PRQLEEMWRDQFDWVYQEMDYATFTITIHPDVSGRPQTLLMLDRLIEHINGHDGVRWA
                     TFNEIADDFLSRFPRS"
     gene            9425..10756
                     /locus_tag="VFE39_10980"
     CDS             9425..10756
                     /locus_tag="VFE39_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259165.1"
                     /translation="MSAVPETGTTPETNTFRRVGVRATRRATVIAFFAWLFAVFDYIM
                     FGTLLPQIASSFGWSEATASLVSTLVSVGTAIIALAVGPVVDRIGRRKGMILTVSGTA
                     VASALSAATPGAGYLIGVRSLGGLGLSEQAVNATYLNEIYAVTDDEVIKKRRGLVYSL
                     VQGGWPLGVLLASAWVAIFLPSIGWRGCFLLAVFPAVLIALMRRGLRESPQFELELQL
                     RALRKEGKTAEATELARSYGVDDQPSAPLTAIFRGQALRNTVVLGFAWFINWFGIQVF
                     SVLGTTVLTEGKHVSFTNSLLLLIVINAVGYLGYVAHGFLGDRLGRRNVIAFGWILSG
                     VLFALMLTVATSTLSVVVLYSLGMFFLVGPYAALLFYMGECYDTRCRATGTGFLNALS
                     QPGAILAGVIVTSMLASGVDWGHAALVVGAVGTFVSGFVMFGARKAAVLQG"
     gene            10771..11526
                     /locus_tag="VFE39_10985"
     CDS             10771..11526
                     /locus_tag="VFE39_10985"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6259166.1"
                     /translation="MSEVAIITGAASGIGRALAVSYAEAGVRTVIGTFPGDPHDPQET
                     AKAVAQAGGECVVHEVDVRDPAQVDAFAQRALDEWGRLDIAVANAGVLRWAALPDLSD
                     AAWDDMLSVDLTGVLRTMRSAAARMDGGAMVAVSSIAGGVYGWGEHAHYAAAKAGVLG
                     LCRSLAVELAPRRIRVNTVIPGYVVTPQSLDPVNSLGPDGLERAGRDIPLGRPGRPDE
                     LASVIRFLTSEQASYVTGQTIIVDGGLTVRMPA"
     gene            11539..12255
                     /locus_tag="VFE39_10990"
     CDS             11539..12255
                     /locus_tag="VFE39_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010314838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6259167.1"
                     /translation="MAELPGEPVLHGRTALVVGGGSGIGRAIAQAFTAAGADVVVADR
                     KNARVYIDVTDEDAVRAAVAETSSVLGRIDVLVNSAGILTESPLMEMDLATWTETIGV
                     DLTGVFLCCRHVVPGMVERGFGRVINIASQLGIKGGRDLAHYSAAKAGVIGLTKSLAL
                     EVAEYGVLVNAIAPGPIETPMVEGITEGWKAAKRRELPLGRFGRPEEVAPTAVLLASD
                     PGGNLFVGQTLGPNSGDVMP"
     gene            12256..12924
                     /locus_tag="VFE39_10995"
     CDS             12256..12924
                     /locus_tag="VFE39_10995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259168.1"
                     /translation="MCGACGDRTPSDWARPWFADLAARAAAVTALLRLVTRPGVRVAA
                     RSGGWLVSGPTGATTVCGGLSALVAAARQWGPAVPEFAPGASGRLAMPEPDGRRGLLL
                     RVDPAAPPTVLRTGLTEVTVPDDASALRQLAAVSTPPWSLRYYLSDVVGVDAPWGRPG
                     DVLTGNAVDVVVWLEWARQAGAFSDRSISARCPLIDGRELDLEIRAGHVVRARSLATG
                     SATS"
     gene            complement(12932..13375)
                     /locus_tag="VFE39_11000"
     CDS             complement(12932..13375)
                     /locus_tag="VFE39_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005461109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6259169.1"
                     /translation="MAQVTARAQRTIEAPAERVRTALADYRTVRPTILTEHYGDYRVE
                     AGGIGAGSRVHWTLQATKNRVRDQLVEVSVPAEDRIVERDANSSMVTTWTVTPAGADR
                     SVVSVETTWNGASGIGGFFERTFAPKGLQRIHDGVLSNLVGAVRE"
     gene            complement(13420..14007)
                     /locus_tag="VFE39_11005"
     CDS             complement(13420..14007)
                     /locus_tag="VFE39_11005"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259170.1"
                     /translation="MRFPRILPIAALAVATLALSACGDIAGGDDGYGYTASPVPLSAP
                     PKPSGDGYGSSSGSGTSVGGTATGGTGTGGVALAARVSPDLGTIVTDAQGFTLYRFDQ
                     DKAKPPTVTCAGDCAAKWPPVVVDPEGQLTLQGVDQAMIGMVQRPDGESQLTINGWAV
                     YRFSGDKTPGTTTGQGVDNVWFAVRPDGRKAATKP"
     gene            complement(14112..>14778)
                     /locus_tag="VFE39_11010"
     CDS             complement(14112..>14778)
                     /locus_tag="VFE39_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259171.1"
                     /translation="WLIEHVWFLREPLDALAGDPDQVVAQAQTWSNVAGALRCVATDH
                     AAAGVYGWSGPAGMAYHGAVGDYARAVNDVAGQADQLANLVLGTGAAVGTVRALIRDL
                     IADFLSRVLRRAIAALATAPVTLGGSTAAAIGAIALEAYRLACHLAERISRLLDVLSD
                     AGSTAGRLVESMRHVVTELRATAPYMQESLEPIPAGEVVEAGKQFSGAELDEQPVEQP
                     PAR"
BASE COUNT         2081 a         5182 c         5357 g         2158 t
ORIGIN      
        1 gcccttcccc gtctcgacat cgatccggcc ggctccgcaa ccgtgccgac cgacgagccg
       61 cccgcgagcg cgcccgcgct catcgtctac acgtccggga cgaccggctc gcccaagggc
      121 gcgatgctct cccgcggggc cctcgcgtcc aatctggacg cgctggccga cgcctgggcg
      181 tggaccgtcg acgacctgct ggtgcacgcg ctgccgctgt tccacgtcca cggtctggtg
      241 ctcggggtca tcgggccgct gcgccgcggc gggacactgc accacatcgg cgcgttggac
      301 gcacggaccc tggccgggag cggggcgtca atggtcttcg gcgtgccgac gcagtaccac
      361 cggctggccg accagctcga gaccgatccg gacgccgccg ccgcgatcgg ccgtgcccgg
      421 ctgctggtgt cgggctccgc ggcgctcacc gccgtggacc acgcccggct gcaccgggct
      481 accggcctcg cggtccgcga gcgctacggg ctcaccgaga cgctgatcct caccgccgcc
      541 cgcgcggagg acgagccgga gccgggaacg gtcgggcgcc cgttgcgcgg caccgaggtg
      601 cggctcacgc cgctggcggg tgagcaggcc gacgatctcg gagcggtcga ggtgcgcggg
      661 ccggggctgt tcgacgggta cctcaaccga cccgacgcga cggcagctgc gcacacgccc
      721 gacggctggt tctgcaccgg tgacatcggg cgctggacgg cgagtggcgc gctggcgctg
      781 gtcggtcgca gcgccaccga cctgatcaag tcgggcgggt acaagatcgg cgcaggggag
      841 atcgagaacg cgctgttgga gcatgcaggc gtggccgagg tcgcggtcgt cggagtgccc
      901 gacgacgacc tgggtgagcg gatcgtcgcg ttcgtcgtgc ccacgggtat cgctcctccg
      961 gagcgggagc tcgtggacca tgtcgccgcg ctgctcgcgc cgcacaaacg ccctcgtgag
     1021 gtgcggttca ccgacgccct gccccgcaac gacatgggca aggtggtgaa ggcccggctg
     1081 ccgcgcgact gatcctgcgc acgtgacctc agccggatga tccgtcgacg cgattgacgg
     1141 cgcgcgggtt cgggcaacct gatcggatga cggacacgac gacccgcacc gggaaccaga
     1201 cctaccgcgg acctgccgga acgggcgcgc cccgcctcgc cgcgctcccc ggcgtccatc
     1261 gggccgacgt cacgccgcag caggtgatcg agatcgagcg atacgacacc gacgaccatc
     1321 ggctcacggc ggcgggcatc accgtcgcgc tgcatcggcg agcggacgag ccgccccagt
     1381 ggcacctcga cctgccggac ggtgacgtgc ccgaacatct gcgcgtcccg ctcgccgacg
     1441 ccgccgggct ctcgcccgca gtgcccggcg agatcgacgc gctcgtgcgt ggggcggcac
     1501 gtgagcgcgc ggtccggccc gtgggccgga ttcggcgcgt gcgtaccgag acccgtctgc
     1561 tcgatgagga cgagcgcctg ctcgcgctcg tcgtgcacga cgaggtcacc ctcgccaccc
     1621 tgggccgcgc caccgacgtc gaggcctgga ccgaggtgga gctccgccgc gccgaggcgg
     1681 acaacgccct cgtcgcggag ctggaggagc ggttcgccga gagcgggttg cgtcccgctg
     1741 tgctcgccgc ggaggaggag ctggagcggc tgctgcgtcc cacgcccaag gcccgccgcc
     1801 tgcgcgccgg caagcgcggt tccgcgggcg ccggggtgat ggagtacctc ggtgcccagc
     1861 tcgaccggtt ggcggccgag gacctgcgcg tccgtcgcga cgaaccggac gccgtgcacc
     1921 agctgagggt tgcgtcccgc cgcctccgca gcgccctgca ggcataccgc ccgctgctgg
     1981 accgcacccg caccgatccg ctcgtggacg gcctccgcga gctgggccgc gagctcgcgc
     2041 ctgcccggga cgccgaggtg ctgcacgagc acatctcgtc cgggctcacc gggctcgcgc
     2101 ccgagctcct gctcgggccc gtgcaggcac agatgacccg gcacttcgcc cgggaacagg
     2161 ccgaggccag ggccgcggtg ctcgccgttc tggacagcga acgacacatg gggctgcggg
     2221 ccgcgctcga cgcgctggtg gagcggccac cgctgaccaa gcgcgcagcc aggcccgccc
     2281 gcaaggaggt gcccgcgctc gtggcccgca ccgctcggag gttggagcgt gcggtccagg
     2341 tcgccaccga ccctgcggtc cccgattccc agcgggacgc cgcggtgcac agcgcccgta
     2401 aggccggcaa gcgcctgcgg tacgccaccg aggtggccaa accggcagtg gggaagcagg
     2461 cgaagaagtt cgcgaaggcg ctcaaaggct tccagtcggc gctcggggag caccaggaca
     2521 cggtggtcgc gcgtggtgcc ctgcgtgagc tgggtgcaat ggcgaactcg gcgggggaga
     2581 acggcttcag cttcggagtg ctgtacggcc aggacgccgc gcgcgccgag cagatcgagc
     2641 gcgacctgcc ggtgctgtgg tcggaggcgt ggacgcgccg caaccgccgc tggctccgct
     2701 gacggatcct ccgccgcacc ccgccacccc cgcgagtcgc ccctttcccg cccgcgagtc
     2761 gcccggttcg gtcccgcgag tcgcccggat tagtcccgcg agtcgcccgg ttcggtcccg
     2821 cgagtcgccg ggtttggtcc cgccagttgc cctgatccgt tccgcgagtt gtccggatca
     2881 agctggcggt cgccccatga gcggttggac ggattgcggg cgggatctcg cgagtcggtc
     2941 cgtgcgacga tcgccgccgt gagcgaaccc cgggacgctg tcgctgatct gcgacggatt
     3001 gccttcctgc tggagcgggc gcacgagtcg acctaccgcg tgcgcgcgtt ccgcactgcg
     3061 gccgcggtgc tcggaaggcg ggacgccgac gagatcgccg gcctggtccg gtcaggtgag
     3121 ctggtgcggc tgaagggcgt cggcgaggtg acggcgcgct gcgtggccga gtcggtcgcc
     3181 ggggaggagc ctgtctacct gcgcaggctg gaggccaccg agggcacgcc gctcgacgag
     3241 gccgccgagg tgctgcgggc ggcgatccgc ggcgactgtc acagccacac cgatgcctcc
     3301 gacggaggct cccccttgcg ggagatggtg cagacggcgc aggagctggg ccacgactac
     3361 ctggtggtca ccgatcactc gccacggctc accgtggcga acgggttgtc ggcggaccgt
     3421 ctgcgcgctc agatcgagca ggtcgccgag ctcaacgtcg agttgggcga cggcttccgc
     3481 gtgctcacgg gcatcgaggt cgacatcaac gaggacggct cgctcgacca ggacgaggat
     3541 ctgctcgccg agctggacgt ggtcgtcgcg tcggtgcact cgaagctccg gatgccgcgg
     3601 gcggatatga cccagcggat gctgacggcc gtcgcgaacc cgcacgtcga tgtgctcggg
     3661 cactgcacgg gccgcatggt cacgggcaac cgcaagcggc cggagtcgca gttcgatgcc
     3721 gacggagtct tcgccgcctg tgcggagtac ggcgtcgcgg tcgaggtgaa ctcccgtccc
     3781 gaacggctcg atccgcccaa gcggctgctg cggctggccg tcgaggcggg ttgctcgttc
     3841 accatcgaca ccgatgcaca tgccccgggt cagctcgact ggcttggcaa cggctgcgag
     3901 cgtgcggccg cgtgtggcgt gccgatggag cgggtgctca acacctggac cgccgagcgg
     3961 ctgctcgcgc gtagtcgatg agcacgcgac gcgcgagagt atcgcggcac tccgatgggg
     4021 gttgctcact ccgtcactca acgtgatatc ggcgcacccg aaccccctag ctgatggatg
     4081 ccccgtgggg aatcggattg ctcgacatcg gcaaccttgc agcttgcgcc ccggtaggaa
     4141 ctcgcccgag ccgtactgaa cacagagtca actcgccggc ggagagcctc cgatagaggg
     4201 gtactcgaca gggtggtcgg cctcgcgctg cgcacgatcc gaagctgaaa tgtcgccgac
     4261 agctttggtt ctggtgatgc aagaggtgac agtggtgcga ttgaggtctc ggtatctgcc
     4321 cgcggcggtg ggggcgtgtc tactgcttgc ggcggccggt ctgccggccc tggcgagccc
     4381 cgcgccttcg gcgcaggagc agatgcccgc cgcggaaacg cccagcctca taccgtctgc
     4441 tgccgcggat cgcgcccgcc agtccgaccc cgactcgacc gaccgaggcg cggacagcac
     4501 cgaaccggca gacgaccagc aggcggacgg caaggactcc ggggccggtt cgggcgacgc
     4561 tggtgctact ggggtgtcgg gcgactccaa tggtgggaac tcggccaaca ccgtgaaccc
     4621 ctccgtttct cccgaccccg ccgaccctgc cgagcccgcc gaccccgccg agcccgccga
     4681 ccctgccgag cccgccgacc cggccgagcc agccgacccg gccgagcccg ccgacgccgc
     4741 ggaccccgcc gagcccgccg gccctgccgg ccccgccggc ccagccggcc cagccgagcc
     4801 cgccgacgct acagagcgcc ccgaccccgg cgaacccacc gatactgcgg accctgccga
     4861 tgctgcggac cccgccgact ccgctagaac tgctagtgcg gctggccctg cgaaccaggg
     4921 tggcccggcg agccccggtg ggcctgcgag cccgggtggt cctgcgagcc cggctggctc
     4981 ggtgagtccg gctggtccgg cgagcccggc cggttcgagg gatccggctg gctcggtgag
     5041 tccggtgagt ccggtgagac cggtaagact ggtaagaccg gtctgtccgg ctggtccgcc
     5101 gagcccggcc ggttcggggg atccggctgg tgcggggagt ccggctggtt cgggggatcc
     5161 ggctggttcg gggagcctgg gtggcccggc gagtccggct gggccggcga gccccgccga
     5221 tccggcgagc ccgcccggcc ctaccgaccc cgccgatcct gccggcccga ccaacgccgg
     5281 gaactccgcc gccggggtac cggccactac gcaggagccc gccgaccctg gggagcgggc
     5341 gcccgctgac ggaccggcgg ccggcgagca gcccgggaat gccgccctgc ctgcggactc
     5401 gtccgaatgc gccacgacgg ccgcagagag gtacgggtgg ggccagcccg cccgccgctc
     5461 tgacttccag gacgggctcc cgcccgactg gcacccctac ggccccgaac ccggccatgc
     5521 caggaggggc actcgcaccc ccgaggcgat cagcgtggcg aacggggtcg ccaccatcac
     5581 tgcgacgccg gacggcacga cgggagcgat gagctggcac cccgggcagc ggtacggccg
     5641 gtgggaggcc tgcgtccgcg cggacgaggg ccagggcggg atcaacgcgc tgatgctctt
     5701 gtggccggtg gcggaggact tcccggtggg cggcgagatc gactggatgg agatcatgtc
     5761 ggatgatcgc caggagacca gctttttcct gcactatggc gagaacaatg atcagactac
     5821 cggctcggtg cggcaccact ccatggagtg gtcggcctat gccctggagt ggacatcgga
     5881 gaagatgacc gcctacgtca acggggacga gtggtactcc accaccgaga ccgacaagtt
     5941 tccgccccgt ccgatgaaca tgacgatgca gttggactac ttcggcgggg cgggcggcgg
     6001 gaccgcgatg cacatggact gggctcagca gtgggcgctg cccgagagcg agcccgccga
     6061 gctgtccctg gcgcccggcg acccggcgac ggggcagccg gatgagtacc cggagcgcgc
     6121 tccccgcgtg ctcgacgggt cgggcgccac cgagtaggtc ggggacccac gagccgtggg
     6181 cggcggggga cgaaaggatc tggggccgtc acccgccgcc cacgaaccac acgtgtcgac
     6241 gtacgccgag ttcaggccct gcgggcgagc aggtcgtcgg tcgtgggcgg gtcggcgccg
     6301 cgccgtgaac aggtgatggc cgccgccgtg acggcctcgt cgagcaggcc gtcgagcgtg
     6361 tcggcgtcga tcgccttcag ctcggggcgg gcggccgcgc ccagcaggcc acgccggtgc
     6421 agcccggcca gaagtgcggc ggagaacgtg tcgccggccc ccaccgtgtc gaccaccggg
     6481 accgggatgc ccgcacgccg ggatcgcacg ccggcggcgg tgccggccag tgcgccgtcg
     6541 ccgccgaggg tgatggcgat caccgcgggt ccacgtgcga gccagtcctc cagcacggcg
     6601 tccggcgtcg agcccggaac gagccactcg aggtcttcgg agctcacctt gaccacgtcg
     6661 gccaccgcga gcagctcgtg ggccccggcg agcacatcgc tcggatcgcc catcagcaac
     6721 gggcggcagt tggggtcgta gctgatcgtg gcggttgcgg cggagcgggt gatcagctcg
     6781 cggagcacgg cggcgccggg cggcgtggtc agcgcgagcg aaccggagtg cagcgcgacg
     6841 acgggcccgt cgagcgcgcc ggtcagttcg tcgggcgtcc actgccagtc ggccgtaccg
     6901 gcgatccgga agtcgtagct cgggccaccg tcggtgtcga cggccaccat cgccatcgac
     6961 gtctgctccc gagcgcgcac ggcgtggtcg agatccaccc cgttgacggt gaggtgttcg
     7021 cggatgcgac ggccgagcat gtcgtcggcg atgcgtgcca gcagccgtgc cggcacaccc
     7081 agccgggaca gtgcaacggc gacgttggcc gggctaccgc cgggcgccat ctcgaagtag
     7141 ccgcctacgg gggccgggac gaggtcgacg agcgcctcgc ccgccacggc gacgatgccg
     7201 gtctcgatcg gtggactgat gaccgctccc ctctccggga cggacccagc gcccgccgcg
     7261 cgtcatgctg gcgggtggac gtgtgtccgc gccactccct gcttcgaccc cgatgaacct
     7321 ccgccacgga gcgccccggt ggtcgagacc cggacgacga gctcggggcg aagctgctcc
     7381 gactccacct gctcaccccg cagcagccga accagcagct cgatcccgcg gcggcccatc
     7441 tcgtgcatcg gcgagcgcac ggtggtcaac ggcaccggca gctcggccgc cagcggagtg
     7501 tcgttgaaac ccgccacggc gatgtccgtg cctgcatgca ggccgtggtc gcggagcgct
     7561 ccgagaacac cgatcgctgc gaagtcgttc acggcgaaga tcgcgctcgg cggtgggccg
     7621 tcggccagca gctgctccgc ggctgcacgg ccgccggtcg cgtcgaaggg cgaccgcacg
     7681 atccgtcgtg gggggatctc gatcccggcc tcggcgtagg cggagacgaa accctgggtg
     7741 cggtcgattc cggtactcgc gtagggctca ccggccacca ccgcgacgtc ccggtgtccg
     7801 agctcgagca ggtgctcgcc gagcaggcgt ccgccgaggt ggtcgttgca ggtcactgac
     7861 ggaaggtcgc ctgcccgccg gttcaccaga acgaacggca cgccgcgcgc cgcgacctcg
     7921 ccgaggaacc gggcgtcgac gtaggcatcg ccgaagatca tcccatcgac gcggcgcgcg
     7981 aggaccatgt ccgtgcggat gcgctggatc tcggggagat cccgggtgtt ggccacgaag
     8041 gtggacagat cgtgctcggc cgcggcctcc tcgatgccct cgtagatcat cgccaggacg
     8101 atgtccgaca gtcgcggaac gagcacgccg atcaggttgc tgcggcgcgt acggaggctc
     8161 gcggcgtgcg ggttgggccg gtagtcgagc tctgtggcga gccgtcggat ccgttcgacg
     8221 gtggccgggg aagcggctcg gccgctttcg tccgacgtgc cgtgtaaagc gcgggagacg
     8281 gtcgacacat gcagcccgag ctggtcggcg agcatccgca gggtgaccgg gggcgttccg
     8341 ttcgcgcaca caggtcgctc cattttccgt aaccgcggtt ttcgcaaccg taacaatcgg
     8401 ttgcattgac atcgtcccgc ccgggctgga acgttacccg aacgtttggg tcgctaacag
     8461 ccccctgcgg atgccatttc tgacgaaagg tccggttcgt gccaaaagaa atcttcgtat
     8521 cgatcggcgt cgatgtcgac gcggtcgccg gctggctcgg ctcctacggc ggagaggact
     8581 cgccgtgtga catctcccgc ggcctcttcg cgggggaggt gggcgtgccc cggttgctgc
     8641 gcctgttcga gcgcaagaac ctgcgcacga cctggttcgt ccccgggcac tcgctggaga
     8701 cgttccccga ccagacccgc atgatcgtcg acgccgggca cgaggtggga gcacacggct
     8761 actcgcacga gaacccgatc gccatgacgc gggaccagga gagcgcgatc ctggaccgca
     8821 gcatcgagct gatcgagaag ctcaccggca accgcccgac cggctacgtc gcgccgtggt
     8881 gggagttctc cccggtgacc aacgagctgc tgctcgagcg gggtgtcaag tacgaccact
     8941 ccttgatgca caacgacttc tccccctact acgtgcgggt gggggacagc tggacgaaga
     9001 tcgactactc caagccggcc gaggagtgga tgaagcccct cgtccgcggc gaggagagcg
     9061 ggctcgtcga gatccccgcg aactggtacc tcgacgacct gcctccgatg atgttcgtca
     9121 aggcgagcgc caacagccac gggttcgtca acccgcgcca gctggaggag atgtggcgcg
     9181 accagttcga ctgggtgtac caggagatgg actacgcgac gttcacgatc accatccacc
     9241 ccgatgtctc ggggcggccg cagacgctgc tgatgctgga ccggctgatc gaacacatca
     9301 acggtcacga cggagtgcgc tgggccacat tcaacgagat cgccgatgac tttctgagcc
     9361 gtttcccgcg gtcctgagag tcccggaatt cccgtttccg accaattctg aaaagggcac
     9421 cgccatgtcc gctgtacccg agacgggtac cactcccgag acgaacacgt ttcgccgagt
     9481 cggggttcga gccactcgga gggcgaccgt tatcgccttc ttcgcctggc tgttcgcggt
     9541 cttcgactac atcatgttcg gcaccctgct gccgcagatc gccagcagtt tcggctggag
     9601 cgaggccacg gcgtcgctgg tctccaccct cgtgtcggtg ggcaccgcga tcatcgcact
     9661 ggccgtcggg cccgtcgtcg accgcatcgg gcgccgcaag gggatgatcc tcaccgtctc
     9721 gggcacggca gtcgcgtcgg ctctctcggc ggcgacgccg ggtgcgggct acctcatcgg
     9781 tgtgcggtcg ctcggcggtc tcggcctgtc cgagcaggcc gtcaacgcca cctacctcaa
     9841 cgagatctac gccgtcaccg atgacgaggt gatcaagaag cgacgcggcc tggtctacag
     9901 cctcgtgcag ggcggctggc cgctcggcgt gctcctggcc tcggcgtggg tcgccatctt
     9961 cctgccgtcg atcggctggc ggggctgctt cctgctcgcg gtgttccccg ccgtgctcat
    10021 cgccctgatg cggcgggggc tgcgcgagag cccgcagttc gagctcgaac tgcagctgcg
    10081 ggcattgcgc aaggagggca agaccgccga ggccaccgag ctggcgcgct cgtacggcgt
    10141 cgacgaccag ccctccgccc cgctgacggc gatcttccgc ggtcaggccc tgcgcaacac
    10201 cgtggttctc ggcttcgcct ggttcatcaa ctggttcggc atccaggtgt tcagcgtgct
    10261 gggcacgacc gtgctcaccg agggcaagca cgtcagcttc accaactcgt tgctgctcct
    10321 catcgtgatc aacgccgtgg gctacctggg ctacgtcgcg cacggcttcc tcggcgaccg
    10381 gctgggccgc cgcaacgtga tcgcgttcgg ctggatcctc tccggggtgc tgttcgcgct
    10441 gatgctgacg gtcgcgacca gcacgctcag cgtcgtggtg ctgtactcgc tcggcatgtt
    10501 cttcctggtc ggcccgtacg cggcgttgct gttctacatg ggcgagtgct acgacacccg
    10561 gtgccgggcg acgggcaccg ggttcctcaa cgcgctgtcc cagccgggtg cgatcctggc
    10621 cggtgtgatc gtcacgtcga tgctcgcgtc gggcgtggac tggggccacg ccgcgctcgt
    10681 cgtcggcgcg gttggcactt tcgtctccgg gttcgtcatg ttcggggccc gcaaagcagc
    10741 ggtgctgcaa ggctgaacga aggagtattc atgtccgaag tcgcgatcat caccggggcg
    10801 gcgagcggga tcggccgagc gctggccgtg tcctacgcgg aggccggggt gcgcacggtg
    10861 atcggcacct tccccggcga cccgcacgac ccgcaggaga ccgcgaaggc ggtggcccag
    10921 gcaggcggtg agtgcgtggt ccacgaggtg gacgtgcgcg atccggccca ggtcgacgcc
    10981 tttgcccaac gtgccctcga cgagtggggg cggctcgaca tcgcggtggc caacgccggg
    11041 gtgctgcgct gggcggcgtt gccggacctg agcgacgccg catgggacga catgctgtcg
    11101 gtcgacctca caggggttct gcgcacgatg cggtcggccg ccgcacggat ggacggcgga
    11161 gcgatggttg ccgtgtcgtc catcgcgggc ggcgtctacg gctggggcga gcacgcgcac
    11221 tatgcagccg ccaaagccgg tgtgctcggg ctctgccgga gcctggcggt ggagctcgca
    11281 ccgcgccgga tccgggtcaa cacggtgatc cccgggtacg tcgtcacacc gcagtcgctg
    11341 gacccggtga actcgctcgg acccgacggc ctggagcgcg ccggccggga catcccgctc
    11401 ggtcgcccgg gtcggccgga cgagttggcg tcggtgatcc ggttcctgac ctcggagcag
    11461 gcgtcgtacg tgaccgggca gaccatcatc gtcgacggcg ggctcaccgt ccgcatgccc
    11521 gcatgaggac ggtgcagcgt ggctgagttg cccggagaac cggttctgca cggccggacg
    11581 gcgctggtcg tcggcggtgg cagcgggatc ggccgcgcca tcgcgcaggc cttcaccgcg
    11641 gccggtgccg acgttgtggt cgccgaccgg aagaacgcca gggtgtacat cgacgtcacc
    11701 gacgaggacg cggtgcgtgc cgccgtcgcc gagacgtcga gcgtgctggg acgcatcgac
    11761 gtcctggtca actccgccgg gatcctcacc gagtcgcccc tcatggagat ggatctcgcg
    11821 acctggacgg agacgatcgg cgtcgacctc acgggagtct tcctgtgctg ccggcacgta
    11881 gtgcccggca tggtggagcg gggcttcggc cgggtcatca acatcgcgtc ccaactcggc
    11941 atcaagggcg ggcgcgacct cgcgcactac tccgcggcga aggcgggtgt catcgggctg
    12001 acgaagtccc tcgccctcga ggtggccgag tacggggtgc tcgtcaacgc gatcgcgccg
    12061 gggcccatcg agacgccgat ggtggagggc atcaccgagg ggtggaaggc cgcgaagcgg
    12121 cgtgagctcc cgctcggccg cttcggccgt cccgaggagg tggcaccgac ggcggtcctg
    12181 ctggccagcg atcccggcgg caacctgttc gtcgggcaga cgctcggacc caactccggg
    12241 gacgtgatgc cctagatgtg cggggcgtgc ggcgatcgga cgccgtcgga ctgggcgcgg
    12301 ccgtggttcg ccgacctcgc cgcgcgggcc gccgcggtca cagccctgct gcggctggtc
    12361 acccggcccg gcgtacgggt cgcggcgcgc agcggcggct ggctggtcag cgggccgacc
    12421 ggcgcaacga cggtgtgcgg cgggctgagc gctctcgtcg ccgccgctcg gcagtggggg
    12481 cccgccgtgc cggagttcgc cccgggcgcg tccggccggc tcgccatgcc ggagccggac
    12541 ggccgcaggg gtctcctcct gcgcgtcgat ccggcagcac cgccgacggt cctgcgtacc
    12601 ggcctcaccg aggtgacggt gcccgacgac gcgtccgcgc tgcggcagct cgccgcggta
    12661 tccaccccgc cgtggtcatt gcggtactac ctgagcgacg tggtcggggt ggacgccccg
    12721 tggggccggc ccggcgacgt gctgacgggc aacgcggttg atgtcgtggt gtggctggag
    12781 tgggccagac aagccggcgc cttctccgac cggtcgatct ccgcccggtg tccgctgatc
    12841 gacgggcggg aactcgacct cgaaatccgc gcaggccatg tcgtgcgcgc gagatcgctg
    12901 gccaccggct cggccacgag ctgagccgga ctcactcgcg gacggctccg accaggttgg
    12961 acagcactcc gtcgtgaatc cgctgcaggc cctttggcgc gaacgtccgc tcgaagaagc
    13021 cgccgattcc gctcgcgccg ttccaggtgg tctccaccga gacgaccgag cggtcggcgc
    13081 ctgcaggcgt gaccgtccac gtcgtgacca tgctggagtt ggcgtcccgc tccacgatgc
    13141 ggtcctcggc cgggacgctg acctcgacca actggtcacg cacccggttc ttcgtcgcct
    13201 gcaacgtcca gtgcacccgg ctgcctgcac cgatcccacc ggcctcgacg cggtagtcgc
    13261 cgtagtgctc cgtgaggatg gtcggtcgca ctgtccggta gtcggccagc gctgttcgta
    13321 cccgctcggc gggtgcctcg atggtgcgct gtgcccgggc cgtcacctgt gccatggacc
    13381 catcctgccc cggcgctccc gcgcggtccg agcggggcgt cacggtttcg tggccgcctt
    13441 cctcccgtcc ggtctgacgg cgaaccacac gttgtcgacg ccctgccctg tcgtcgtgcc
    13501 aggagtcttg tcgcccgaga agcggtagac cgcccacccg ttgatcgtga gctggctctc
    13561 gccgtcgggt cgctggacca tgccgatcat ggcctggtcc acgccctgca gcgtgagctg
    13621 gccttccgga tcgacgacga ccggcggcca cttcgccgcg cagtcgcccg cgcacgtgac
    13681 ggtcggcggc ttggccttgt cctggtcgaa gcggtagagc gtgaagccct gtgcgtccgt
    13741 gacgatcgtg ccgaggtcgg gcgacacgcg cgccgccagc gcgacgccgc cggtgcccgt
    13801 gccgccggtg gccgtgccgc cgacggaggt gccggagccc gagctggatc cgtagccgtc
    13861 gcccgatggc ttggggggtg ccgacagcgg gacgggggac gcggtgtagc cgtaaccgtc
    13921 atcgccaccg gcaatgtcgc cgcaggcgct gagtgcgagg gtggcgacgg cgagcgcggc
    13981 gatcggaagg atgcggggaa agcgcatggg gagctcctcg atcggggcca gttcggggct
    14041 ggccgactcc tgacacacgg gtgccgggcg gggacggttc atccgatttc ggcaagtggc
    14101 cgtcggggtg attaccgggc gggcggctgc tccacgggct gctcgtccag ctctgcgccc
    14161 gagaactgct tgcccgcctc gaccacctcg ccagcgggaa tcggttcgag ggactcctgc
    14221 atgtacggcg ccgtggctcg gagctcggtg accacgtgcc gcatgctctc gacgagccgg
    14281 ccggctgtgc taccggcgtc cgacagcacg tcgagcagcc gggagatgcg ctcggcgagg
    14341 tggcaggcga ggcggtaggc ctccagcgca atggcaccga tcgccgccgc ggtcgaccct
    14401 ccgagcgtga ccggcgcggt cgccagcgcc gcgatcgccc gtctgagaac cctgctgagg
    14461 aagtcggcga tgaggtcacg gatcagggcg cggacggtgc cgaccgcagc tcccgtgccg
    14521 agcaccaggt tcgcgagctg gtcggcctgc ccggccacgt cgttgacagc acgtgcgtag
    14581 tcgcccaccg caccgtggta agccatgccg gcgggcccgc tccacccgta cacccctgct
    14641 gcggcgtgat cggtcgcaac gcaccggagc gcaccggcga cattgctcca ggtctgcgcc
    14701 tgggcgacca cctggtcggg gtcgccggcc agtgcatcga ggggctcgcg aaggaaccag
    14761 acgtgctcga tgagccaa
//
LOCUS       DASRSC010000223        12430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4341630,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000223 DASRSC010000000
VERSION     DASRSC010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12430
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4341630"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..926)
                     /locus_tag="VFE39_11015"
     CDS             complement(153..926)
                     /locus_tag="VFE39_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6259172.1"
                     /translation="MRRVSKRVSPWFLVLLAVTVGGGALTVIGTPEALISGGSVLLTA
                     GVVLLVLGGWAVSLCLHEFGHAAVAYRGGDLSVRDKGYLTLDIRRYTDVGLSFVLPIV
                     FLLLGGIPLPGGAVWINHGAIRSRAMRSLVSFAGPATNIVIGALLTAAVALVPMPIGL
                     AIGLSCLALIQVLAFVLNILPVPGLDGFGVLEPYLSLPARQLAARVRPWAPIVLFLLI
                     IGVPGVSQLFFEIGGAIYSAVGGDFNLAAQGYSQLLFWR"
     gene            1100..1432
                     /locus_tag="VFE39_11020"
     CDS             1100..1432
                     /locus_tag="VFE39_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET6259173.1"
                     /translation="MNVITATMELVETTAGVPFPAADAFGEAVVPLGQDEADEQDWQE
                     RALCAQTDPEAFFPEKGGSTREAKRICSGCEVRAECLDYALAHDERFGIWGGLSERER
                     RRLRRAVS"
     gene            complement(1625..2104)
                     /locus_tag="VFE39_11025"
     CDS             complement(1625..2104)
                     /locus_tag="VFE39_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HET6259174.1"
                     /translation="MTTARRTLRRSPRRRDRRGRGLRGLMYPATTPASRTRAEKFDAL
                     VLEALEPIELRWGSELVDLDLAVDEVPEVDETSPDDVVWGQGVLADVGVPLAQLVPAG
                     VDPEGLPSRARIVLYRRPLEARARGGADLADLLHEVLVEQVAEYLNIEPDAVDGGES"
     gene            2204..2551
                     /locus_tag="VFE39_11030"
     CDS             2204..2551
                     /locus_tag="VFE39_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 domain-containing protein"
                     /protein_id="HET6259175.1"
                     /translation="MRRCSRTGCTELAVATLTYVYADSTAVVGPLATQAEPHSYDLCT
                     KHAHRLTAPLGWEVVRFEGEFAPPPQSNDDLTALAEAVREAGRVDRPVEAVPTTGTGR
                     RGHLRVLPGPPDD"
     gene            2606..3961
                     /locus_tag="VFE39_11035"
     CDS             2606..3961
                     /locus_tag="VFE39_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HET6259176.1"
                     /translation="MANLSAIVKAYDIRGVVGEQLDVPTVRDIGAAMASLVRTEGASG
                     VVVGHDMRASSPELAAAFAEGVTGQGLDVISIGLASTDMLYFASGTLHLPGAMFTASH
                     NPAKYNGIKLCRAGAAPIGQDTGLSAIREAVEAGVSAGPGGGTVTERELLDEYAAYLR
                     GLVDLSDGRRLRVVVDAGNGMGGYTVPAVLAPLALDVVSMYFELDGTFPNHEANPLDP
                     ANLVDLQKRVLIEGADIGLAFDGDADRCFVVDELGEPVSPSAITALVAVRELAKSPGS
                     AVIHNLITSRAVPEIVTEHGGRPVRTRVGHSFIKQTMAETGAVFGGEHSAHYYFREFW
                     NADSGMLAALLVLAALGEQDRPISQLMSVYGRYAASGEVNSTVPDQADRIAAIKQTYG
                     SVDGVELDELDGLTVQLPGGSWFNLRASNTEPLLRLNVEAADSVAVAALRDEVLAVIR
                     A"
     gene            4081..4302
                     /locus_tag="VFE39_11040"
     CDS             4081..4302
                     /locus_tag="VFE39_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HET6259177.1"
                     /translation="MAVELDPQLMDILACPSEDHAPLLAGTPTDPHADVLTCTACGRR
                     FPVVDGVPVLLLDEAITPADPPSPGTAGD"
     gene            4305..5465
                     /locus_tag="VFE39_11045"
     CDS             4305..5465
                     /locus_tag="VFE39_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259178.1"
                     /translation="MLDDILLADPRALAALDTGGVLRAAATAGAQVRSAAQGATDAGV
                     GDLGGTRPRALVLLRRPGASGPAAGLLCALLGPASPMPIVVVDAVPGWIGPLDVVVGH
                     TADADDAELADGIAFAVRRGAEVVLSAPIDGPVASAGAGRVRLVEQRIPVPPGLDLPR
                     ALAVGLAVVGALGLLAAPLDSAMGPLADQLDAEAERNQPGHEPFMNPAKSLALRLAEH
                     TPLLWGTDPLGAAVAEHAAAALATHAGVVAHADDVGRAATATGLLRALDLASAGADVF
                     HDPFDDDADGGAQAPPVRLVLLATGEDDPGHVTLRVAGRRWPSADVVHPVDEVARGVP
                     HAGLLRAGLLASRFDVAAVYLGLATRTNDPEYRDDARSGGARFSTGATSEES"
     gene            5473..6693
                     /gene="manA"
                     /locus_tag="VFE39_11050"
     CDS             5473..6693
                     /gene="manA"
                     /locus_tag="VFE39_11050"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET6259179.1"
                     /translation="MELLDNPVRPYAWGSRTVIADLLGQEIPSPHPQAELWFGAHPAD
                     PSRLVHPDGSRTSLLDALRADPEHLLGADRSGHWDATLPFLLKVLAADEPLSLQAHPS
                     IEQARTGFAREEAAGIPRDAADRNYRDANHKPELICALTEFHALVGFREPAATVRLLR
                     ALEVPELAGHAELLAAQPDQDGLRALFTTWITLPQSMLDVLVPVLQEGCIRLAGSGGD
                     FHTEARTVLELSERYPGDAGVLAALLLNRVTLAPGEALYLPAGNLHAYLSGAGIELMA
                     NSDNVLRGGLTPKHVDVPELLRVLDFAAAPPPVLTGRPEGGWIRYDTPAKEFLLRRWA
                     GGTGEPVAVPDGGPRILLCTAGAACVRSPGGEMQLARGSSLWLAAAETGITMHRQAED
                     TELFLASDGLSSPR"
     gene            6834..7754
                     /locus_tag="VFE39_11055"
     CDS             6834..7754
                     /locus_tag="VFE39_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET6259180.1"
                     /translation="MAATGGTRAIVAALLANAGIAVAKFIGYLITGSSSMLAEAVHSV
                     ADTSNQGLLLLGGRQAKRAATEEHPFGYGRDRYFYSFVVALLLFTLGSVFALYEGIHK
                     LGSHEPLTSPLVAVLILVVAIGLESWSFRTAIHESRPLKGSGTWWQFIRQSKVPELPV
                     VLLEDLGALVGLALALLGVGLTVLTGNSVFDAIGTICIGVLLGVIAIILIVEMKSLLI
                     GEGATSPVLTKIVSAIESGDVERVIHIKTQYLGPDELLVAAKVALPPGRPVEDVARAI
                     DEAEARIRAAVPEARVIYLEPDLDRTPVAS"
     gene            7865..9415
                     /locus_tag="VFE39_11060"
     CDS             7865..9415
                     /locus_tag="VFE39_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6259181.1"
                     /translation="MAVGDRQSGIMRTKSVEQSIADTDEPDSRLRKDLGTWDLIVFGV
                     AVVVGAGIFTLAASTAGNVAGPSVSLAFVLAAIACGLAALCYAEFASTVPVAGSAYTF
                     SYATFGELIAWIIGWDLVLEFAVGSAVVAKAWSEYLATVLGLTFGVEVPTSVDVGPVT
                     LDWGALLLVGVLTVLLALGTKLSSRVSLVFTTIKVVIVLLVIVVGIGYVNVANYSPYI
                     PPAAEGGDAASGLQQSLLSVLGGGASSTYGVFGLLAAASLVFFAFIGFDVVATTAEET
                     KDPQKSLPRGIFGSLAIVTVLYVAVALVLTGMVRYTELQTQPDGTRATLATAFESLGV
                     TWAATVIAVGALAGLTTVVMVLMLGQIRVLFAMARDGLLPRGLSRTSSVRGTPARATI
                     LVGAVVAAGATFFPIGALEEMVNIGTLFAFVLVSVGVVILRRTRPDLHRGFRTPLVPL
                     VPVLSVLACLWLMLNLTVETWLRFVVWMLIGLVVYALYGRTHSRLGLRDGADSEAVSV
                     RKESRNMP"
     gene            9482..10825
                     /locus_tag="VFE39_11065"
     CDS             9482..10825
                     /locus_tag="VFE39_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0569 family flavin-dependent
                     oxidoreductase"
                     /protein_id="HET6259182.1"
                     /translation="MVRSVHDGDHHPVVVVGGGQAGLSMSWWLTQRSIGHVVLERDRV
                     ATEWRERRWDTFSLVTPNWQCDLPGHPYAGGDADGFMVRAEIVAYLEAYAASFGAPVV
                     ERTEATRLRGRRAGGFEIRTTAGTLTADQVVVAAGPYQVPIRPRMAERLSTGVVQIHS
                     SEYRNPDQLPPGEVLVIGTGQSGCQIAEDLHLAGRRVHLATGSAPRVARLYRGRDVVA
                     WLHDMGYYRKSVTEFDDADAVRFRVNHYVTGRDGGRDIDLRAFARDGMRLYGRLAGVD
                     GGLLRFAPNLRQHLDAADAVSESIKDSIDTFIAAHGIHAPTEPRYVPVWEPADEPTEL
                     DLDAAGITGVVWSTGFGRDHRFIELPIFDGRGYPTHERGVTNWPGLYFLGLPWLHTWG
                     SGRFSGVGADAEHLAREVVAHQHRTVHTGLHWLAGTPYQTSPADDEEWIAPRTVA"
     gene            10856..11368
                     /locus_tag="VFE39_11070"
     CDS             10856..11368
                     /locus_tag="VFE39_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0572/Sll0783 family nitrogen starvation
                     response protein"
                     /protein_id="HET6259183.1"
                     /translation="MADLTDLEKQSLEEIQHPALPEGSTIYGGTKVFPDYQAENGETY
                     FTLVHGIAHESSVSFVAILQATRALRKGFESAIYFYGPGSLNCLATRGFPTTGNSAFP
                     GEHNINNSLGTFIQEGGKVYCCRFGLSLHGAREEDLIEGVIPTHPLDVQDALIHYARK
                     GAIINSTYMF"
     gene            11485..12336
                     /locus_tag="VFE39_11075"
     CDS             11485..12336
                     /locus_tag="VFE39_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET6259184.1"
                     /translation="MASLRMAAVAAPFGRDLEEDLARISALVADARASGVRLLALPEA
                     CLGGYLASLDGPEDRPPALRIDGPEIRTLAMLAREMVVVAGYCEQDDAGVRYNSAVCV
                     TGDGVLANYRKVHQPLREDLSYGAGDRFAAFDSPVGRLGMQICYDKAFPEAARALALD
                     GAQIVVSISAWPLSATDPHPDPAQDRWMRRFDLFDRARALENQIVWLAANQSGTFGSL
                     RFASGAKVVDPGGEVLATTGVRAGTAVAELDVAAVVESARRAMGYLRDLRPDAYGSAA
                     APRPAAA"
BASE COUNT         1764 a         4461 c         4382 g         1823 t
ORIGIN      
        1 gatcagcgtc gagtccgacg atctgcggtg gtggccgctc gatgcgctcc ccgatgacag
       61 cgatgcagtg gcgacggtgg tggccgccgc gcgcggacgc cgggccgcca ctcccgcctg
      121 ctgaccacgc cggaagtccc cctcgacgcg cgtcagcgcc agaagagcaa ctggctgtag
      181 ccctgcgcgg cgaggttgaa gtcaccgccg accgcggagt agatcgcgcc gccgatctcg
      241 aagaacagct gcgacacccc gggcaccccg atgatcaaca ggaacagcac gatcggtgcc
      301 cacgggcgta cccgcgccgc gagctgccgc gcgggcaggg acaggtacgg ctcgagtacg
      361 ccgaacccgt cgaggcccgg caccggcagg atgttcagga cgaacgcgag cacctggatc
      421 agcgccaggc aggacagccc gatcgcgagc ccgatgggca tcggaaccag tgcgacggca
      481 gccgtgagta acgctccgat gacgatattg gtggcgggac cggcgaacga caccaagctc
      541 cgcatggcgc gcgagcggat cgcgccgtgg ttgatccaca ctgcgccacc cggtagcgga
      601 ataccaccca gcagaaggaa cacgatgggg agcacgaacg acagtccgac atccgtgtag
      661 cggcggatgt cgagagtgag ataacccttg tcccgtaccg acaggtcgcc gccgcggtac
      721 gcgacggccg catgtccgaa ctcgtgcagg cacagcgaca ccgcccagcc gccgagcacc
      781 agcagtacga cacctgccgt gagcagcact gatccgcccg agatgagcgc ttcgggagta
      841 ccgatgacgg tcagtgcgcc accgccaacc gtcacggcca ggagtaccag gaaccacggg
      901 ctgaccctct tcgaaactcg gcgcaccaga ccattgtgct gatcgggcga ccgaccgggt
      961 ggagtgcatc acccggatgg cagcttcagc ttgccaaggc ccgactgaca gcgctgtact
     1021 tacatggctg ttattcaagg ggtgggggcc gatggatccc ctcgggcagc cattcgccag
     1081 ggtgcgggga ggtgggcgag tgaacgtgat cacagccacg atggaactgg tcgagacgac
     1141 ggctggtgtg ccgttcccgg ccgccgacgc gtttggggag gcggtcgtcc cgctcggtca
     1201 ggatgaggcc gacgagcagg attggcagga gcgcgcgctc tgcgcgcaga ccgatccgga
     1261 ggcgttcttc cctgaaaagg gtggctccac tcgcgaggcc aagcggatct gctcgggctg
     1321 cgaggtgcgg gcggagtgcc tggactacgc gctcgcccac gacgagcgct tcgggatctg
     1381 gggcggcctg tccgagcgcg agcggcgtcg cctgcgccgc gcggtctcct gacggctccc
     1441 tggtcgccag aaggcgccga accgcgggtg ccaccccgca gcacccggac caacaggaag
     1501 ggcggccggc agccgtgcag gtggatcgac cagcagggca agagccgctc actcgtgtga
     1561 ttcaaggcgg ggatccccga gctgaggcgc accggcggtg ccgcggggac acgcgcaccg
     1621 cccgctagga ctccccgccg tccacggcgt cgggctcgat gttgaggtac tccgccacct
     1681 gctccacgag cacctcgtgg agcaggtcgg ccaggtccgc gccgccccgg gcccgggcct
     1741 cgagcgggcg gcggtacagc acgatccggg cgcgggacgg cagtccctcg gggtcgacgc
     1801 ccgccggcac cagctgggcg agcggaacgc cgacgtcggc cagcactccc tgaccccaca
     1861 ccacgtcgtc gggcgaggtc tcgtccacct cgggcacctc gtcaacggcg aggtccagat
     1921 ccacgagctc actgccccag cgcagctcga tcggctcgag cgcctccaac accagcgcgt
     1981 cgaacttctc ggcccgcgtg cgggaggccg gcgtggtggc cggatacatg agcccacgca
     2041 gtccacggcc gcgccggtct cgccggcggg gcgagcggcg cagggtgcgg cgcgcagtgg
     2101 tcactcgcgc gatcctacgg ctctcggccc gccctgctcg acgcccgcgc gtcgcggtgg
     2161 gatgaagcgc ggtgcaagcg ctatcgtcgg ggacgtgctg agtgtgcggc ggtgttcgcg
     2221 gaccgggtgc accgagctcg cggtggccac gctcacctac gtctacgccg actccacggc
     2281 agtcgtaggt ccactggcca cgcaggccga accgcattcc tatgacctgt gcaccaaaca
     2341 cgcgcatcgg ctcacggcgc cgcttggctg ggaggtggtg cggttcgagg gcgagttcgc
     2401 cccgccgccg caaagcaatg acgacctgac ggcgctcgcc gaggccgtcc gcgaagctgg
     2461 ccgggtggac cgcccggtcg aggccgtgcc caccaccggc accggccgcc gcggccacct
     2521 gcgggtgctg cccgggcccc cggacgactg aggggtcgtc cgactgcctg cgccgggaat
     2581 ccgccgcccg ctagggtctt cccccgtggc aaacctgtcg gcgatcgtga aggcctatga
     2641 catccgcggc gtcgtcggcg agcagctcga tgtcccgacc gtgcgggaca tcggcgccgc
     2701 catggcctcc ctcgtgcgta cggagggcgc gagcggcgtc gtcgtcggtc acgacatgcg
     2761 cgccagctcc ccggagctgg cggccgcgtt cgccgagggc gtcacggggc agggcctgga
     2821 cgtcatctcc atcgggctcg ccagcaccga catgctctac ttcgcctccg gcacgctcca
     2881 cctgccgggc gcgatgttca cagccagcca caacccggcg aagtacaacg ggatcaagct
     2941 gtgccgtgcg ggcgccgcgc cgatcggaca ggacaccggg ctgagcgcga tccgggaggc
     3001 cgtcgaggcc ggggtatcgg ccggccccgg cggcggcacc gttaccgaac gtgaactgct
     3061 cgacgagtac gccgcctatc tgcgcgggct cgtcgacctg tcggacggcc gccgcctgcg
     3121 ggtcgtggtc gatgccggca acgggatggg cgggtacacc gtgcccgccg tgctggcgcc
     3181 gttggcactg gacgtcgtgt cgatgtactt cgagctcgac ggcaccttcc ccaaccacga
     3241 ggccaacccg ctcgaccccg ccaacctggt ggacctgcag aagcgggtgc tgatcgaggg
     3301 cgccgacatc gggctcgcct tcgacggcga cgccgatcgc tgcttcgtcg tcgacgagct
     3361 cggcgagccc gtgagcccca gcgcgatcac cgcgctggtg gccgtccgcg agctggcgaa
     3421 atcgcccggt tccgccgtca tccacaacct gatcacgtcc cgggccgtac cggagatcgt
     3481 caccgagcac ggggggcggc cggtccgcac ccgggtgggc cactcgttca tcaagcagac
     3541 gatggccgag accggcgctg tcttcggcgg tgagcactcg gcgcactact acttccgcga
     3601 gttctggaac gccgactccg ggatgctcgc cgcgctgctc gtgctggccg cgctcggcga
     3661 gcaggaccgc ccgatctcgc agctgatgag cgtctacggg cggtacgccg cgtccggtga
     3721 ggtcaactcg acggttcccg accaggccga ccggatcgcc gcgatcaagc agacctacgg
     3781 gagcgtcgac ggcgtcgagc tcgacgagct cgacgggctc accgtccagc tgcctggcgg
     3841 ctcctggttc aacctgcgcg cgtccaacac cgagccgctg cttcggctca atgtcgaggc
     3901 cgccgactcc gtcgccgtgg ccgccctgcg cgatgaggtg ctcgcggtca tccgcgcata
     3961 gcggacggcc tccaccggta ccagccggtc ggatggacca ggcgcggagc gcttcgccct
     4021 ggcagcatcg cgggcaacgg atctgcccgc ggagccgtcc cgcagagaaa gaggaagacc
     4081 gtggccgtag agctcgaccc ccagctgatg gacatcctgg cctgcccgtc cgaagaccac
     4141 gcgccgctgc tggccggtac ccccaccgat ccgcacgccg acgtgctcac ctgcacggcg
     4201 tgcggccgtc gcttccctgt cgtggacggc gtcccggtgc tgctgctcga cgaggcgatc
     4261 acgcccgccg atccgccttc ccccggcacg gccggcgact gacggtgctc gacgacatcc
     4321 tgctcgccga tccgcgggcg ctcgccgctc tcgacacagg tggcgtgctc cgtgcggccg
     4381 ccaccgcggg cgcccaggtg cgttcggcgg cgcagggcgc caccgacgcg ggcgtcggcg
     4441 acctgggcgg gacgcggccg cgtgcactgg tgctcctgcg caggccgggc gcgtccggtc
     4501 cggcggcagg cctgctctgc gcactgctcg ggccggcgtc cccgatgccg atcgtggtcg
     4561 tcgacgcggt gccgggctgg atcggaccgc tggacgtcgt cgtcggccac accgccgacg
     4621 ctgacgatgc cgaactcgcc gacgggatcg cgttcgcggt gcgtcgtggc gcagaggtcg
     4681 tgctgtccgc acccatcgac ggccccgtgg cctcggccgg ggcgggccgc gtccggctgg
     4741 tggagcaacg gatcccggtc ccgccggggc tcgacctgcc gcgggcgctg gccgtcgggc
     4801 tcgccgtggt gggcgcgctc ggcctgctcg ccgccccgct ggactccgcg atgggaccgc
     4861 tggccgatca gctcgacgcc gaggcggagc gcaaccagcc cggccacgag ccgttcatga
     4921 accccgcgaa gtcgctcgcg ttgcgtctcg ccgagcacac gccgttgctg tggggcaccg
     4981 atccgctcgg cgccgccgtc gccgagcacg ccgctgccgc actggcgacg cacgcgggcg
     5041 tggtggcgca cgccgacgac gtcggccggg ccgccaccgc caccggcctg ctccgggcgc
     5101 tggacctggc gtccgcagga gccgacgtgt tccacgaccc gttcgacgac gacgcggacg
     5161 gcggtgcgca ggcacctccc gtgcgcctgg tgctgctcgc caccggagag gacgaccccg
     5221 gtcacgtgac gctccgggtc gccggtcgtc gctggccgtc ggccgatgtg gtgcacccgg
     5281 tggacgaggt cgcccgtggc gtcccgcatg cgggactgct gcgggccggg ctgctcgcgt
     5341 cgcgcttcga cgtggccgcg gtgtacctgg ggctggccac ccgcacgaac gatccggagt
     5401 accgagacga cgcccgctcc ggcggcgcgc gcttctccac cggtgctacg tctgaggaga
     5461 gctgacgaga tcgtggagct gctggacaac ccggtacgac cgtacgcctg gggatcacgc
     5521 acggtgatcg ccgacctgct gggtcaggag ataccgtcac cgcaccccca ggccgagctc
     5581 tggttcggtg cgcacccggc cgacccgtcc cggctggtgc accccgacgg gtcgcgtacg
     5641 tccctgctcg acgccctgcg cgccgacccg gagcacctgc tcggcgccga ccgcagcggt
     5701 cactgggacg cgaccctgcc gttcctgctc aaggtgctgg ccgccgacga gcccctctcg
     5761 ctgcaggcgc atcccagcat cgagcaggcg cgcaccgggt tcgccaggga ggaggccgcg
     5821 ggcatccccc gtgacgccgc cgaccgcaac taccgcgatg cgaaccacaa gcccgagctg
     5881 atctgcgcac tcaccgagtt ccacgccctc gtcgggttcc gcgagccggc ggccacggtg
     5941 cggttgctgc gcgcgctcga ggtcccggag ctggccgggc acgccgagct gctcgccgcg
     6001 cagccggacc aggacggcct gcgcgcgctg ttcaccacgt ggatcacgct cccgcagtcg
     6061 atgctcgacg tcctggtgcc cgtgctgcag gagggctgta ttcggctggc cgggagcggt
     6121 ggcgacttcc acaccgaggc acgcacggtg ctggagctgt ccgagcgcta ccccggtgac
     6181 gcgggcgtgc tggccgcgct gctgctcaat cgcgtcacgc tggcgcccgg cgaggcgctg
     6241 tacctgcccg caggcaacct gcacgcctac ctgtccggtg ccggtatcga gctgatggcc
     6301 aactccgaca acgtcctgcg cggcggactc acgcccaagc acgtcgacgt gcccgaactg
     6361 ctccgggtgc tcgacttcgc cgcggcacca ccgccggtgc tcaccggacg cccggagggc
     6421 gggtggattc gctacgacac cccggcgaag gagttcctgc tgcgccgctg ggccggtggc
     6481 accggggaac cggtcgccgt gcccgacggc ggcccgcgga tcctcctgtg cacggcgggc
     6541 gcggcctgcg tccgctcgcc gggcggagag atgcagctcg cccgcggctc gtcgctgtgg
     6601 ctggcggcgg ccgaaacggg tatcacgatg caccggcagg ctgaggacac ggagctgttc
     6661 ctggccagcg acggcctcag ttcgccgcgc taggcgccca cgctgccgta ggtcacccat
     6721 ggcccagcgc ctcaccggga tcgtcgacca ggcgattctg cccgcggtcc acgagcgccc
     6781 ggacaagcgc tagggtcccg ggcgcagcag gcccgcaggt cgaggagagt gcagtggcag
     6841 caactggtgg aacccgggcg atcgtggcgg ccctgctggc caacgcgggg atcgcggtcg
     6901 ccaagttcat cgggtacctg atcaccgggt cgtcctccat gctggcggag gcggtgcact
     6961 cggtggccga cacgtcaaac cagggcctgc tgctgctcgg cgggcggcag gcgaagcgtg
     7021 cggcgacgga ggagcacccg ttcggctacg gccgcgaccg gtacttctac tcgttcgtgg
     7081 tggccttgct gctcttcacg ctcgggtcgg tgttcgcact ctatgagggc atccacaagc
     7141 tggggtccca cgaaccgctg acgtcgccgc tcgtcgccgt cctgatcctc gtcgtcgcga
     7201 tcgggctcga gtcgtggtcc ttccgcacgg cgatccacga gtcccggccg ttgaagggct
     7261 cgggaacctg gtggcagttc atccgccagt ccaaggtgcc cgagctgccg gtggtgctgc
     7321 tggaggacct cggcgcgctc gtcggtctcg cgctcgccct gctcggcgtc gggctcaccg
     7381 tgctcacggg caactccgtg ttcgacgcga tcggcacgat ctgcatcggc gtgctgctcg
     7441 gcgtcatcgc gatcatcctg atcgtcgaga tgaagagcct gctgatcggc gagggcgcca
     7501 catcgccggt gctcacgaag atcgtctccg cgatcgagtc cggtgacgtc gagcgggtca
     7561 tccacatcaa gacccagtac ctcgggcccg acgagctgct cgtggctgcg aaggtcgcac
     7621 tgccgccagg gcgcccggtc gaggacgtcg cccgggccat cgacgaggcc gaggcccgga
     7681 tccgggcagc cgtgccggag gcccgcgtca tctacctcga gcccgacctc gaccggacgc
     7741 cggtggcaag ctgacccgcc tgccgcctgc tcgacgccca gcagtcgatc gtgggacgag
     7801 ctgtaccgga ccgctctgtc gctagcggtg gtccggttca tcaccctggg ttagtttctc
     7861 cgccatggct gtgggagaca ggcagagcgg gatcatgcgg acgaagtccg tggagcagtc
     7921 cattgccgac acggacgagc ccgacagccg gctccgcaag gatctcggca cctgggacct
     7981 gatcgtcttc ggcgtggcgg tggtggtggg ggccggcatc ttcacgctgg ctgcgagcac
     8041 ggcgggcaac gtcgccggac cgtccgtgtc gctggcgttc gtgcttgccg ccatcgcctg
     8101 cggactcgcc gcgctgtgct acgcggagtt cgcctccacc gtgcccgtgg cgggcagcgc
     8161 ctacaccttc tcctacgcca ccttcggcga gctcatcgcg tggatcatcg gctgggacct
     8221 ggtgctggag ttcgccgtcg gctccgcggt agtggccaag gcctggtcgg agtacctggc
     8281 gacggtgctg ggcctgacgt tcggcgtcga ggtgccgacc tcggtcgacg tcgggccggt
     8341 cacgctggac tggggagccc tgctgctcgt cggggtgctc accgtgctcc tcgccctcgg
     8401 cacgaagctg tccagccgag tcagcctcgt cttcaccacg atcaaggtgg tgatcgtgct
     8461 gctggtgatc gtcgtgggca tcggctacgt caacgtcgcc aactacagcc cgtacatacc
     8521 gccggccgca gagggcgggg acgccgccag tggcctgcag cagtccctgc tctcggtgct
     8581 cggcggcggg gccagcagca cctacggcgt gttcggcctg ctcgcggcgg cctcgctggt
     8641 gttcttcgcg ttcatcgggt tcgacgtcgt ggccaccacc gcggaggaga cgaaggaccc
     8701 gcagaagtcg ctgccccggg gcatcttcgg gtcactcgcg atcgtcaccg tcctctatgt
     8761 ggccgtcgct ctggtgctca ccggcatggt ccgctacacg gagctgcaga cgcagcccga
     8821 cggcacgcgc gccaccctgg ccacggcgtt cgagtcgctg ggcgtgacgt gggccgccac
     8881 cgtgatcgcg gtcggcgcgc tcgccgggct caccaccgtg gtcatggtgc tcatgctggg
     8941 ccagatcagg gtgctgttcg cgatggcgcg tgacgggctg ctgccccggg gcctctcccg
     9001 cacgagcagc gtgcggggca ccccggcccg ggcgacgatc ctcgtcgggg ccgtggtggc
     9061 cgctggtgcc acgttcttcc cgatcggcgc gctggaggag atggtcaaca tcggcacgct
     9121 gttcgcgttc gtgctggtgt cggtgggtgt cgtgatcctg cgccggaccc ggcccgacct
     9181 gcatcgcggg ttccgcaccc cgctggtgcc gctggtgccg gtgctgtccg tgctcgcatg
     9241 tctctggctg atgctcaacc tgacggtcga gacgtggctg cggttcgtcg tctggatgct
     9301 gatcggcctc gtcgtgtacg cgctctacgg ccgcacgcac tcccggttgg gcctgcgaga
     9361 tggggccgac agcgaggctg tgagcgttcg gaaagaatca cgaaacatgc cctaacgtcg
     9421 gcgtgttccg ggggaaaccc cctagtgaca tgcggccggt ctaatcgact gtgtgatcga
     9481 gatggtgcgg agcgtccacg acggggacca tcaccccgtc gtcgtggtcg gcggcggcca
     9541 ggccggactg tcgatgagct ggtggctgac ccagcggtcg atcgggcacg tcgtgctgga
     9601 gcgcgaccgg gtggccaccg agtggcgcga gcgccggtgg gacacgttct ccctggtcac
     9661 gcccaactgg cagtgcgacc ttccgggcca cccgtacgcg ggtggcgacg ccgatggctt
     9721 catggtccgg gccgagatcg tcgcctacct ggaggcgtac gccgcgtcct tcggcgcgcc
     9781 cgtcgtcgag cgcaccgagg ccacccggct gcgcggtcgc agggcaggcg gcttcgagat
     9841 ccggaccacg gccggcacgc tcaccgccga ccaggtcgtc gtcgcggccg ggccgtacca
     9901 ggtgccgatc cggccgcgga tggccgagcg gctgtctacc ggcgtcgtcc agatccactc
     9961 ctcggagtac cgcaatcccg accagctgcc gccgggggag gtgctggtca tcggaaccgg
    10021 gcagtcgggc tgtcagatcg cggaggacct gcacctggcc ggccgccggg tgcaccttgc
    10081 cacgggcagc gcgccccggg tcgcgcgcct ctaccggggc cgggacgtcg tcgcctggct
    10141 ccatgacatg ggctactacc gcaagagcgt caccgagttc gacgacgccg acgccgtgcg
    10201 gttccgggtc aaccactacg tcaccggccg agacggcggc cgcgacatcg acctgcgcgc
    10261 cttcgcccgc gacggtatgc ggctctacgg gcggctcgcg ggcgtcgacg gcgggctcct
    10321 gcggttcgcg ccgaacctgc ggcagcacct cgacgcagcc gacgcggtgt ccgaaagcat
    10381 caaggactcg atcgacacct tcatcgccgc ccacgggatc cacgccccga ccgagccgcg
    10441 atacgtcccg gtctgggaac ccgccgacga accgaccgag ctggacctgg acgcagccgg
    10501 aatcaccggc gtcgtgtgga gcaccgggtt cggccgagac caccgtttca tcgaactgcc
    10561 gatcttcgac gggcgcggct atccgaccca cgagcgcggc gtcacgaact ggcctggcct
    10621 gtacttcctc gggctgccgt ggctgcacac gtggggctcg ggccgcttct ccggagtggg
    10681 cgccgacgcc gagcacctcg cccgcgaggt cgtcgcccac cagcaccgca ccgtgcacac
    10741 cggcctgcac tggctggcag gcaccccgta ccagacctcg ccggccgacg acgaggaatg
    10801 gattgctcca cgcaccgtcg catagcccgt cccaccacct cacgaaggag cactcatggc
    10861 cgacctgacc gatctcgaga agcagagcct cgaggagatc cagcacccgg cgttgccgga
    10921 gggctcgacg atctacggag gcaccaaggt cttccccgac taccaggccg agaacggcga
    10981 gacctacttc acgctcgtgc acggcatcgc ccacgagtcg tcggtgagct tcgtggcgat
    11041 cctgcaggcc acgagggcgc tgcggaaagg cttcgagtcg gcaatctact tctacgggcc
    11101 cggctcgctc aactgcctgg ccacccgcgg gttcccgacc acgggcaact ccgcgttccc
    11161 cggcgagcac aacatcaaca actcgctggg gacgttcatc caggagggcg gcaaggtcta
    11221 ctgctgccgc ttcgggctct cgctgcacgg cgcccgcgag gaggacctca tcgagggcgt
    11281 catccccacg cacccgctcg acgtgcagga cgcattgatc cactacgccc gcaagggcgc
    11341 gatcatcaac tcgacctaca tgttctgagc gggcgggctt cgacgggcag gggatggggt
    11401 gggggctgcc cgagggcggc tcccacccct tcgtcggccg gaccgaccac ccccgaggac
    11461 caacccaagg acggagcacg agagatggca tcgctgcgca tggcggcggt cgcggccccg
    11521 ttcggccgtg acctggagga ggacctcgca cggatctccg ccctggtggc cgacgcgcgc
    11581 gcgtccggcg tgcggctgct ggcgttgccg gaggcgtgcc tgggcggcta cctggcgagt
    11641 ctggacggcc ccgaggaccg gccacctgcg ctgcgcatcg acgggccgga gatccgcacg
    11701 ctggcaatgc tggcccgcga gatggtggtg gtggccggtt actgcgagca ggacgacgcg
    11761 ggcgtccgct acaacagcgc ggtctgcgtg accggcgacg gcgtgctcgc caactaccgg
    11821 aaggtgcacc agccgctccg cgaggacctg agctatggcg caggcgaccg cttcgccgcg
    11881 ttcgactcgc cggtcggcag gctgggcatg cagatctgct atgacaaggc attccccgag
    11941 gcggcgcgag cgctcgcgct cgacggcgcc cagatcgtcg tctcgatctc ggcctggccg
    12001 ctgagcgcca ccgaccccca tcccgatccg gcgcaggacc ggtggatgcg ccgcttcgac
    12061 ctcttcgacc gggccagggc actggagaac cagatcgtct ggctcgcggc gaaccagagc
    12121 ggcacgttcg gctcgctgcg gttcgcgtcc ggcgcgaagg tcgtcgaccc cggcggcgag
    12181 gtgcttgcca ccaccggtgt cagagcgggc actgcggtag cagagctcga cgtcgcggcg
    12241 gtggtggagt cggcccggcg agccatggga tacctgcgcg acctgcgacc ggatgcgtac
    12301 ggctcggcgg cggctccccg ccccgcggcc gcctgaacac tcggggcgtg acagcgatcc
    12361 ggatcggagc cgtggctgcc ggcttcggca gggacctgac gttcggcctc tcccgcatcg
    12421 cgacgttggt
//
LOCUS       DASRSC010000224        16728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4341692,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000224 DASRSC010000000
VERSION     DASRSC010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 16728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16728
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4341692"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..918
                     /locus_tag="VFE39_11080"
     CDS             220..918
                     /locus_tag="VFE39_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6259185.1"
                     /translation="MSRPTEHEVLAAVLQIRAGRLQVLAWQRARPPDSGRWALPGGRL
                     GDDEDVERSVRRQLAEKVDVREVTHVEQIAVFSNPDRVPGPRLVATAFLGLVPSDSDP
                     AVPADTTWHPLSVLPPTAFDHTAIVEAAHDRLRAKLSYTNLGFALAPREFTISELRGL
                     YVAALGHPVSATNLQRVLTRRRLLEPTGEVAPPGPAGGRPAALFRFTDRTLRVTDAFA
                     VLRPPSEQLRSAVP"
     gene            923..1600
                     /locus_tag="VFE39_11085"
     CDS             923..1600
                     /locus_tag="VFE39_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HET6259186.1"
                     /translation="MAETLPLFPLGTVLMPGASLPLHIFEPRYRQLTVDLVTGAVPDK
                     EFGVVAVREGWAPDDDGLAGLHSVGCTAELRDVRRLPDGRFDLVTRGARRFRLLDLDE
                     ESKPYLMGSVEFLPDTDTPVADGMRQMLTTAARAAHRRYCDTAWKAGDWSEPESDVEP
                     DTLPHVLAADCLLPIGDRQRLLEQTSPIERLRMVRALLARETGLLSQLRAVPAPITTF
                     AVETSVN"
     gene            complement(1735..2226)
                     /locus_tag="VFE39_11090"
     CDS             complement(1735..2226)
                     /locus_tag="VFE39_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HET6259187.1"
                     /translation="MANWSSVLRQASKIGLDLLSSRKQAPAAPPRLPARTASTVERAR
                     TIIYSPDPDGTPDPGEIVWAWVPFEEDDGRGKDRPLLVVGRQGNELVGLMLSSQQDRS
                     GDWDWISIGSGAWDREGRESFVRLDRVLEVGEHDIRREGAILDRHRFERVAAELRTRF
                     GWR"
     gene            complement(2340..2921)
                     /locus_tag="VFE39_11095"
     CDS             complement(2340..2921)
                     /locus_tag="VFE39_11095"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6259188.1"
                     /translation="MSAGAQIISAPDLRWSATWEDDVTDDEHRVLAALFARCFPRSRT
                     VFTGARSWSSGRPELRLVGRSDGRAVAHVGVLRRFLRVAERSASVLVGDVGLVAVDPD
                     VQGRGFGAQLLGRTASTLHDLELPFGYLTCGDHVAAFYATAGWMRVDGGTRMVRADSR
                     VQVYGGVSMVLPVHAPIQEWPAGCLVDRNGLEI"
     gene            complement(3090..4097)
                     /gene="bioB"
                     /locus_tag="VFE39_11100"
     CDS             complement(3090..4097)
                     /gene="bioB"
                     /locus_tag="VFE39_11100"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HET6259189.1"
                     /translation="MTTTETGILATARTKVLEEGTGLSEAQVLDVLRLPDDALDELLE
                     LAHQVRMRWCGPEVEVEGIISLKTGGCPEDCHFCSQSGLFDSPVRSAWLDVPSLVEAA
                     KQTAKTGATEFCIVAAVRGPDARLMTQVAAGIAAIKDEIDINIACSLGMLTQEQVDDL
                     AAMGVHRYNHNLETARSHFPNVVTTHTWEERWETLRLVREAGMEVCCGGILGMGESLE
                     QRAEFAGQLAALTPDEVPLNFLNPRPGTPFGDLEPMAGPDALRTVAAFRLALPRTVLR
                     FAGGREITLGDLGARKGLLGGINAVIVGNYLTTLGRPAESDIDLLGELQMPIKALNET
                     L"
     gene            complement(4094..4831)
                     /gene="bioD"
                     /locus_tag="VFE39_11105"
     CDS             complement(4094..4831)
                     /gene="bioD"
                     /locus_tag="VFE39_11105"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HET6259190.1"
                     /translation="MTREGARSENATDAGILVVTGTGTGVGKTVVTAAVAALACARGD
                     RVAVLKPAQTGVGAGEPGDLADIARLVPSVTSRELARYPDPLAPATAARRAGTPPVTP
                     AAAAEAARELAADHDLVLVEGAGGLLVRLTDEGGTLADVAALLGAPVLVVATAGLGTL
                     NHTALTVEALRSRTLTCAGVVVGAWPAEPDLATRCNRTDLPAITGVPLIGVLPDGMGA
                     LLPEEFADAARRGLASALGGEWQTAVP"
     gene            complement(4828..5979)
                     /locus_tag="VFE39_11110"
     CDS             complement(4828..5979)
                     /locus_tag="VFE39_11110"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HET6259191.1"
                     /translation="MHPFPPSHPLAWLAPHAARRREAGLRRELRPRSASDGLLDLAGN
                     DYLGLTIDPRVIGGALEALHTWGAGSTGSRLVTGTTTLHAELERELAAFCGAESALVF
                     SSGYAANLGLLTALSGPDALIVSDAANHASLVDACRLSRARVEVVPHDDPAAVDAVLA
                     GRPEPRALVVTESVNSVDGGLSPVRELYAVCRNRGALLVVDEAHGLGVVGPGGRGLLA
                     QVGLAGADDVVVTVTLSKALGSQGGAVLGPAAVTAHLVDTARSFIFDTGLAPASVGAA
                     LAALQVLRAEPDRPVAVLRAAAELADAAGVAAPPSAVVSVVLGDPVRAVEAARACAAL
                     GVRVGCFRPPSVPEGTSRLRLTARATLTEADTALVRAVLAEVLAPAPAG"
     gene            complement(6144..6527)
                     /locus_tag="VFE39_11115"
     CDS             complement(6144..6527)
                     /locus_tag="VFE39_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6259192.1"
                     /translation="MPASAEAWVHWRTGSRAFRPACSAVARAADGEIVGYVLSYEHDA
                     DTARTGRGDLYVATVGTIAAYRGRGVAAELLARVLQAARDQGYDTSSLTVDAHNPTGA
                     LGVYDRAGYRLNRREITFALPPTHP"
     gene            complement(7260..8540)
                     /locus_tag="VFE39_11120"
     CDS             complement(7260..8540)
                     /locus_tag="VFE39_11120"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HET6259193.1"
                     /translation="MTIDDLLAADRAHVWHPYAPMPASVAPLAVESAAGVRLRLADGR
                     ELVDGMSSWWAAIHGYAHPVLDAAVRDQLGSMSHVMFGGLTHGPAVDLARLLVDITPA
                     GLQHVFLADSGSVSVEVAIKMCLQYWRSRGRPEKHRLLTWRGGYHGDTFGAMSVCDPD
                     GGMHGLWRDVLPQQVFAGRPPATFETAYVAELAELVERHAAELAAVIVEPVVQGAGGM
                     RFHDSRYLHVLRELCLTYDVLLVFDEIATGFGRTGELFAADHPGVAPDVMCVGKALTG
                     GYLTMAATLCTGEVARGITEGEAGVLMHGPTFMGNPLAAAVAAASIRLLLGGDWRARV
                     HGIEAALRNGLAPARNLPGVVDVRVLGAIGVVQLDREVDVAAATAAAVDAGVWLRPFR
                     DLVYTMPPYICGPAELTAIGAAVVAAAATPHHSP"
     gene            8592..9455
                     /locus_tag="VFE39_11125"
     CDS             8592..9455
                     /locus_tag="VFE39_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HET6259194.1"
                     /translation="MSTAAESATAHPALSRRRELGATSARSLLLTVLGEFVLPRDDAV
                     WTQVLIDVLGGLGVETKSARQALARTAAEGLLVSDRAGRRVRWALSEQGRRLLSDGAA
                     RIYGFGAAARPWDGRWLVLLVSVPEARRQLRHRLRTRLAWAGLGSPAPGVWLTPDPTK
                     QDEVAEVVADLELTGVSSSFVGPFGAIGAQLQVVEQAWNLDEVETAYEEFLETFACAT
                     PTDPADVLAHQIHLVHTWRRFPFLDPKLPGELLPAGWAGARAAELFDSLHSRWDEPAQ
                     QHWLRLVGTAS"
     gene            9565..9897
                     /locus_tag="VFE39_11130"
     CDS             9565..9897
                     /locus_tag="VFE39_11130"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HET6259195.1"
                     /translation="MAAYAVFFSYTPQAWERMITKPGDRAGAARAMIESAGGTLESLY
                     FMLGDRDGFAVVHVPGNEDVAALSIAIASSGAFSQVETRALVAPQDLAGVLEKAAAAR
                     GAYTPPGE"
     gene            complement(10100..10315)
                     /locus_tag="VFE39_11135"
                     /pseudo
     CDS             complement(10100..10315)
                     /locus_tag="VFE39_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010988951.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            10559..11449
                     /locus_tag="VFE39_11140"
     CDS             10559..11449
                     /locus_tag="VFE39_11140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259196.1"
                     /translation="METLAGAWALILWAVLVMIVSVIRRRRCLRDAARPIRRGRQAAL
                     VAGALVIAAVAPAVGAWHGRFAFGLLLAGPIFCLVMAAGSLATELLVRPVAAPRRRAT
                     LAARHFPQYVDRPQLIAVCALFAVAVGFLTTTAFFGDADDKGRPGRWLFLHNFNPDPR
                     FGGSIILWPGAYYALPLLAALGLTLLMTSIALRRIAARPRTVTADGAADPVVADERAR
                     RRSATVVLAAAGVAAAAALCGAGMVAASGLAGAEAPWNVLGMLLWVVVAAAAAVGAWS
                     AAVLVIPDRARRPHPAPATA"
     gene            11446..11865
                     /locus_tag="VFE39_11145"
     CDS             11446..11865
                     /locus_tag="VFE39_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6259197.1"
                     /translation="MTGDPVVEIDLDDPTPPYAQLRRGLAALIEQGTLAGGQRLPTVR
                     QLAGDLGIAAGTVMRAYKELESAGYVRGDRRRGTVVIPRERDPVDVDAALRALAVAYC
                     HRAEDVGATPEQTLAAVSRVLADDHAAVVTRGGSASG"
     gene            complement(11890..15435)
                     /gene="dnaE"
                     /locus_tag="VFE39_11150"
     CDS             complement(11890..15435)
                     /gene="dnaE"
                     /locus_tag="VFE39_11150"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET6259198.1"
                     /translation="MARPSSGDSFVHLHTHTEFSMLDGAARLDDLFKEAARMEMPALA
                     MTDHGNVYGAYEFWRKAKAHGVKPIIGMEGYLSPGSRHERKRATLGGQTVSDDNPGEM
                     YTHMTLLAENTEGMHNLFRLSSLASLEGYYYKPRMDRELLETYGKGIIATTGCPSGEV
                     QRLLQQDKFDAACQAASDYRDIFGKDNFFCELLDHGIAIERQVRDALYDLKKKLDLPG
                     LATNDLHYTYAEDAKSHEVLLCVQTGKTLADPGRFKFDASDFYLKSPAEMRAQWDPVF
                     PEACDNTLLIAERVDVGFTEGQDLMPRAPVPEGETEESWLIKEVERGMHMRFPNGITQ
                     QYRTQVEYELGVIIQMGFPGYFLVTADLIQHAKSVGIRCGPGRGSAAGSLVAYALGIT
                     DLDPIKHKLIFERFLNPDRISMPDIDMDFDERRRGEMIRYTTEKYGEDRIAQIITYST
                     IKAKAAIKDSARVLFGQPGYAVADRISKAMPAAVMGKDIPLSGLFDPSHKRYSEATEV
                     RALYESDPQVKEIIDTARGLEGLKRQWGVHAAGVIMSSKPLIDVIPIQRRDSDGAIIT
                     QFDMGACETLGLLKMDFLGLRNLTIIDDALENIQLNGKPALVIESLELDDPTTYELLG
                     RGDTLGVFQLDGGPMRDLLRRMAPTEFDDISAVGALYRPGPMGANAHNDYADRKNKRQ
                     DVTPIHPELAEPLKDILGETYGLIVYQEQVMAIAQKLAGYSLGKADLLRRAMGKKKKE
                     ILDKEYAGFAQGMKDNGYSEAAVKTLWDILLPFSDYAFNRAHSAAYGVVSYWTAYLKA
                     NYPAEYMAAVLTSVRDDKDKAAVYLAECRRMGITVLAPDVNDSVRNFAPVGNDIRFGL
                     GGIRNVGVNVVDAIVAARKEKGAFTDFSDFLRKVDAVVCNKKVIESLIKAGAFDSLGH
                     PRKGLLLVHADAIDAVMSTKKAASMGQFDLFGSMDGSGPSELDSVFDVKVPTDEWDAK
                     HKLAVEREMLGLYVSGHPLHGVEQVLTAKSDMAISTILEGTVPDGHQVTIGGILANVN
                     RRVNKNGEPWASAQLEDLAGGIEVLFFPRCYQVVGVDVAEDAIVLVKARVNRRDDRVS
                     LIVNDLAVPDLTHAGGSAGAPVKVSMRTELCTPQRVSKLKDVLSNHPGTSEVHLSLVN
                     GTRATVLRLDDALKVTHSPSLMGDLKALLGPGCLG"
     gene            15773..16693
                     /locus_tag="VFE39_11155"
     CDS             15773..16693
                     /locus_tag="VFE39_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6259199.1"
                     /translation="MSRPSPYASLVARVPVREDEVVLRGGRTRLRVYGKDDAAYRMVF
                     LHGLRGDHHGLEPIVAHLRDVRVVVPDLPGFGESPPLPDDTHDVAGYAAWTRELLAAV
                     APGGDAVLAGHSFGSIVAAAAVSHCGPEPVPSTRVRGLVLVNPIAASALAGPRVVMTQ
                     LAVLYHRLAAALPERAGTALLRNRAMTRVSSVAMVTTVDPQLRRWIHGEHDRYFSTFA
                     DRRTLLEAFHASVRHDVGEFAHAVTVPTLLVAAERDDIAPPAAQRALAGRFADVRLVM
                     VPGTGHLAHYEAPDAVAAAMSAFLAALPVP"
BASE COUNT         2298 a         6109 c         5899 g         2422 t
ORIGIN      
        1 ggacctcctc cgcccacgcg gcgtcgaacg tcggggcggt gacgtcggtg agcgtcacga
       61 tgtcctcctc ctgttttcga ctataagtcg taaacaggcc gatcgtagca gcgggccctc
      121 gctcctacac cgttctcgtg accgggaact catcccggcg caacccgccg ttcatgtgtc
      181 cgcgcgggcc tccgttgccg cgacgcctac catccgcgca tgagccgccc gaccgagcac
      241 gaggtgctgg cggcggtgct gcagatccgc gcaggcaggc tgcaggtgct ggcctggcaa
      301 cgagcgcggc cgccggattc gggccggtgg gcgctgcccg gcggccggct cggcgacgac
      361 gaggacgtcg agcggtcggt ccgacgccag ctcgcggaga aggtcgacgt ccgtgaggta
      421 acccacgtcg agcagatcgc ggtgttcagc aaccccgatc gggtgcccgg ccctcgtttg
      481 gtcgcgaccg cgttcctcgg gctcgtgccg tccgactccg atcccgcagt gcctgccgac
      541 accacgtggc acccgctcag cgtcctgccc ccgacagcgt tcgaccacac cgccatcgtc
      601 gaggccgccc acgaccgcct gcgggccaag ctgtcctaca cgaacctcgg gttcgcgctc
      661 gccccccgcg agttcacgat ctccgagttg cgcgggctct atgtggccgc gctcggccac
      721 cccgtgtccg cgaccaacct gcaacgcgta ctcacccggc gccggctcct ggagcccacc
      781 ggcgaggtcg cccctcccgg ccctgcaggc ggacgtcccg cagcgctctt ccggttcacc
      841 gacaggaccc tccgcgtgac cgacgccttc gcggtactcc ggccgccgag cgagcaactg
      901 cgcagcgccg taccgtagtc gggtggccga gacgctcccg ctgttcccgc tgggcacggt
      961 gctcatgccc ggggcctcgc tgccgctgca catcttcgaa ccgcgctacc ggcagctcac
     1021 cgtcgatctc gtcaccggcg ccgtgccgga caaggagttc ggcgtcgtcg ccgtccgcga
     1081 gggctgggcc cccgacgacg acggcctcgc cggactccac tccgtcggct gcacggccga
     1141 gctgcgcgac gtacgcaggc tgcccgacgg gcgcttcgac ctcgtcaccc gcggcgcgcg
     1201 gcgcttccgg ctgctcgacc tcgacgagga gtcgaagccc tacctgatgg ggtcggtgga
     1261 gttcctgccc gacaccgaca cgcccgtcgc cgacgggatg aggcagatgc tcaccaccgc
     1321 ggcccgagcc gcacaccgcc gctactgcga cacggcgtgg aaggccgggg actggtccga
     1381 gccggagtcg gacgtggagc cggacacctt gccccacgtg ctcgcggccg actgtctgct
     1441 cccgatcggc gaccggcagc gcctgctgga gcagacctcc ccgatcgagc ggctgcggat
     1501 ggtgcgcgcc ctgctggcac gcgagaccgg cctgctcagc cagctccgcg ccgtgcccgc
     1561 cccgatcacg accttcgcgg tggaaaccag cgtcaactaa ccccacccgc cgccccaccc
     1621 cacgcgagtc gcccgcccca ggcccgcgag tcgccccgtt tccgcccgcg agtcgcccac
     1681 cccaggcccg cgagtcgccc gatctccgcc cgcgagtcgc ccgggatcgg cgtgtcagcg
     1741 ccagccgaag cgcgtccgga gctccgccgc cacgcgttcg aaccggtgcc ggtccaggat
     1801 cgcgccctcg cggcggatgt cgtgctcgcc gacctccagc acccggtcga gccggacgaa
     1861 cgactcccgc ccctcccggt cccatgcacc ggatccgatc gagatccagt cccagtcgcc
     1921 ggagcggtcc tgctggctcg acaacatcag gccgacgagc tcgttgccct gtcgcccgac
     1981 gaccagcagc ggtcggtcct tgccccgccc gtcatcctcc tcgaacggga cccacgccca
     2041 cacgatctcg ccggggtcgg gcgtgccgtc ggggtcgggc gagtagatga tcgtgcgcgc
     2101 ccgctccacg gtcgatgcgg tccgggccgg cagtctcggc ggagcggccg gcgcctgctt
     2161 gcgcgacgac agcaggtcca gaccgatctt cgaggcctgc cgtaggacgc tgctccagtt
     2221 cgccatgagc acagcatgaa cgacggaccg gcaggccctg tcgtcccccc atgatcgacg
     2281 gaacgtggca gcggatcatc gcctcgctgg gcctgcacgc tcccgctacg gcgaccgggt
     2341 cagatctcca gcccgttgcg gtccaccagg cacccggcgg gccactcctg aatcggcgcg
     2401 tgcaccggca gcaccatcga caccccgccg tagacctgca cccggctgtc cgcacgcacc
     2461 atccgtgtcc cgccgtccac gcgcatccag ccggccgtcg cgtagaacgc agcgacgtga
     2521 tcgccgcagg tcaggtaccc gaacgggagc tccaggtcgt gcagggtcga cgcggtccga
     2581 ccgaggagct gcgcgccgaa ccccctgccc tgtacgtccg gatcgacggc gacgaggccg
     2641 acgtccccga cgagaaccga ggcgctccgc tcggcgacgc gcagaaaccg ccgcaggaca
     2701 ccgacgtgcg cgacggctcg cccgtccgag cgaccgacga gccgcagttc cggtcggccg
     2761 ctggaccagc tccgggcgcc ggtgaacacg gtacgtgacc gcgggaagca gcgggcgaac
     2821 agcgcggcga gcacccgatg ctcgtcgtcg gtgacgtcgt cctcccaggt cgcggaccag
     2881 cggaggtcag gtgcggagat gatctgcgct ccggcgctca ccgtggcggc tgcaccgcgc
     2941 ggtccagctg gtgggcgtca cctgtaccac catgcgccgg gcgcattgcg ggcagaaccg
     3001 tggcggttcg agctcccgtc gggcggcgca gcgcaggtgg tcaccctcct cggagtgcgc
     3061 gccgcactgg tcgcagaaca ccggcaccgt cacagcgtct cgttcagcgc cttgatcggc
     3121 atctgcagct cgccgagcag gtcgatgtcg gactccgccg ggcggccgag cgtcgtcagg
     3181 tagttaccga cgatcaccgc gttgatcccg cccagcagcc ccttgcgcgc gccgaggtcg
     3241 ccgagggtga tctcccggcc gccggcgaag cgcaggacgg tgcggggcag tgccaaccgg
     3301 aacgccgcga cagtgcgcag agcgtcgggt cctgccatcg gctcgaggtc gccgaacggg
     3361 gtgccggggc gcgggttgag gaagttgagc ggcacctcat cgggggtcag cgctgcaagc
     3421 tgcccagcga actccgcccg ctgctccagg ctctccccca tccccaggat cccgccacag
     3481 cacacctcca tgccggcctc gcgtaccagc cgcagcgtct cccagcgctc ctcccacgtg
     3541 tgggtggtga cgacgttcgg gaagtgcgag cgggcggtct cgaggttgtg gttgtagcgg
     3601 tggacgccca tcgctgcgag gtcgtcgacc tgctcctggg tgagcatgcc cagcgagcag
     3661 gcgatgttga tgtcgatctc gtccttgatc gcggcgatgc ccgcggcgac ctgcgtcatc
     3721 agccgggcgt ccggcccgcg gacggcagcg acgatgcaga actcggtggc gcccgtcttg
     3781 gcggtctgct tggccgcctc gacaagtgac gggacgtcga gccacgccga gcggaccggc
     3841 gagtcgaaca agcccgactg ggagcagaag tggcagtcct cgggacaccc gccggtcttg
     3901 agcgagatga tcccctcgac ctcgacctcc ggaccgcacc atcgcatccg cacctggtgc
     3961 gcgagctcga ggagctcgtc gagcgcgtcg tcaggcagcc gcagtacgtc gagcacctgc
     4021 gcctcggaca ggcccgtgcc ctcctccaac accttcgtgc gtgcggtggc caggatgccg
     4081 gtctcggtgg tcgtcacggg acagcagtct gccattcgcc gcccagcgcc gaggccagcc
     4141 cgcgacgcgc ggcgtcggcg aactcctcgg gcagcagagc gcccatcccg tcggggagca
     4201 ccccgatcag cggtacgccg gtgatggcgg gcagatctgt gcggttgcac ctcgtggcga
     4261 ggtcgggctc ggcaggccag gccccgacga ccacccccgc gcacgtcaac gtgcgggacc
     4321 ggagggcctc gacggtcagc gcggtgtgat tgagcgtgcc gagacccgcg gtggccacca
     4381 ccagcacggg agcgccgagc agagcggcga cgtcggcaag tgtgccgccc tcatcggtga
     4441 gcctgaccag caaccctccg gcaccctcga ccagcacgag gtcgtggtcc gcggccagct
     4501 cccgcgccgc ctcggcggcc gccgcaggcg tgacgggcgg tgtgccggcg cgccgggcgg
     4561 cggtggcggg tgcgagcggg tcggggtagc gggcgagctc gcgcgacgtg acgctcggga
     4621 cgagccgggc gatgtcggcc agatccccgg gctccccggc gccgacgccg gtctgggcgg
     4681 gcttcagcac ggccacccgg tccccgcggg cgcaggcgag cgcggccacc gccgcggtga
     4741 cgaccgtctt gccgaccccg gtccccgtcc ccgtcacgac gaggatgccc gcgtcggtgg
     4801 cgttttccga ccgcgccccc tcgcgggtca tcccgccggc gcgggggcca gtacctcggc
     4861 caggaccgcg cggaccagcg ccgtgtccgc ctccgtgagg gtcgccctgg ccgtcagccg
     4921 cagccggctc gtcccctccg gtaccgaggg cggtcggaaa cagccgaccc gcaccccgag
     4981 ggcggcgcag gcccgcgccg cctcgaccgc ccgcaccgga tcgccgagca ccaccgacac
     5041 caccgccgaa gggggagccg ccaccccggc cgcgtcggcc agctccgcgg cagcccgcag
     5101 caccgccacc ggccgatccg gctcagcccg gagcacctgc agagcggcga gcgccgcgcc
     5161 gacggacgcg ggggcgaggc cggtgtcgaa gatgaacgag cgtgcggtgt cgacgagatg
     5221 tgcggtgacc gcggccggcc cgagcacggc tccgccctgg ctgccgagcg ccttggagag
     5281 cgtcacggtg acgaccacgt cgtccgcccc ggcgagtccc acctgtgcga gcaggccgcg
     5341 cccgccggga ccgaccacac ccagtccgtg cgcctcgtcg accacgagca gcgcgccgcg
     5401 gtttcggcag acggcgtaca gctcgcgcac cggggagagt ccgccatcga cgctgttgac
     5461 gctctcggtg acgacgagcg cccgcggctc gggtcgaccc gcgagcacgg cgtcgacggc
     5521 ggccgggtcg tcgtgcggaa cgacctcgac ccgggcacgc gagagccggc aggcgtccac
     5581 gagcgaggcg tggttggcgg cgtcggagac gatcagcgcg tcgggacccg acagcgccgt
     5641 cagtaggccg aggttcgcgg cgtagccgga ggagaagacc agcgccgact ccgccccgca
     5701 gaacgccgcg agctcgcgct ccagttcggc gtgcagcgtg gtggtgcccg tgaccaggcg
     5761 ggacccggtg gagcccgcgc cccacgtgtg cagcgcctcc agcgcgcccc cgatcacgcg
     5821 gggatcaatg gtgaggccga ggtagtcgtt cccggccagg tcgagcaggc catcggaggc
     5881 gctgcgcgga cgcagctcgc ggcggagccc ggcctcccgg cgacgggcgg cgtgcggagc
     5941 cagccaggcc agcgggtgcg agggcgggaa ggggtgcacg ggcgaccacg ttaacgaccc
     6001 ggctcatgcg tccggaatgg gcgactcccg acggacggtg cgccctgcgc gcacaggccc
     6061 cccggtgggc gactcgcgta cggatgctga gcgcgggccg cggccggcgc accgcaggcg
     6121 gcaacgtcct tgccgcagcg gggtcaggga tgggtcggag ggagggcgaa ggtgatctcc
     6181 cggcggttca ggcggtaacc cgcccggtcg tagacgccca gggcaccggt cgggttgtga
     6241 gcgtcgacgg tgagcgagga cgtgtcgtaa ccctgatcac gggccgcctg caacacgcgc
     6301 gcgagcagtt cggcagcgac tccgcggccc cggtacgccg cgatcgtgcc gaccgtggcg
     6361 acgtacaggt cgcccctgcc ggtgcgcgcc gtgtcggcgt cgtgctcgta gctgagtacg
     6421 tagcccacga tctcgccgtc tgctgccctc gccaccgccg agcaggccgg acggaaggcc
     6481 cggctcccgg tgcgccagtg cacccatgcc tcggcgctcg ccggcactga accccagtgg
     6541 tcggcacagg cgctgttgtg cgcggcgcgg agcggctcgt ccagcgcgcg gcgtcgtagt
     6601 acgggcccag cgacgccacg gccaacccgg gcggcgcggg aatgggcgcg accggatcgg
     6661 tcagttcgcg gtggagctcc gaccaccacc ggaccggccc catcgcgttt tcctcggcca
     6721 gcgcgacggc gcccgcgttg gtctcgggca cccgcatgac caggatcgcg ccgagctcgc
     6781 ctgcccgctc gcgcgccacg gccagcagcg cgttcccaat tccccgacgc cggtacgcgg
     6841 gatgcacgcc cgcgtccgag atcaggtgcg caccctctgc gcgtccgctg cgcagccgca
     6901 gcacctgata ggccatagcc gtgctctcgt cgcccgcggg cccgtcgagc acgacgatgg
     6961 tgtcgcgttc caggtcgagc tcgggatcag cgagctcctc ggcacagtcg tcggcgtcgt
     7021 agtgcaaatc acgccggtcg acctcctcga ccgcggccag cagttcggcc caccgcccgg
     7081 cgtcgtcggg gctcatcgga cggccgcgca atccggaccg gagcgccggc gggaggacag
     7141 gcagggccac ggccgcgacg ctaggacatc ccggctcgag cacgcacacg gtttgcaccc
     7201 ggcgactcgc gggcggaaac ctggcgactc gcgggcgggg gacgggcgac tcgcgggggt
     7261 cagggactgt ggtggggggt tgctgctgcg gccaccaccg cggccccgat cgccgtgagc
     7321 tccgccggtc cgcagatgta gggcggcatc gtgtagacga ggtcgcggaa cggccgcagc
     7381 cacaccccgg cgtcgacggc cgccgccgtc gccgcggcca cgtccacctc gcgatcgagc
     7441 tggacgacgc cgatcgcacc gagcacgcgg acgtccacga cgccgggcag gttccgggcc
     7501 ggggccagac cgttgcgcag ggccgcctcg atgccgtgca ccctggcccg ccagtccccg
     7561 cccagcagca gccggatcga cgctgccgcg accgcggccg cgagcgggtt gcccatgaac
     7621 gtcgggccgt gcatcagcac gcccgcctcg ccttcggtga tcccgcgcgc gacctccccg
     7681 gtgcagagcg tggcagccat cgtcaggtag ccgccggtca gggccttgcc gacgcacatc
     7741 acgtcgggcg cgacgccggg gtggtcggcc gcgaacagct cgccggtgcg gccgaaaccg
     7801 gtggcgatct cgtcgaacac cagcagcacg tcatacgtga ggcacagctc gcgcaacacg
     7861 tgcaggtagc gcgagtcgtg gaaccgcatg ccgcccgccc cctgcaccac cggctccacg
     7921 atcaccgctg ccagctcggc ggcgtgccgc tccacgagct cggccagttc ggcgacgtag
     7981 gccgtctcga acgtcgccgg cggccggccc gcgaacacct gctgcggcag gacgtcgcgc
     8041 cacagcccgt gcatgccgcc atcggggtcg cagacgctca tcgcgccgaa cgtgtcgccg
     8101 tggtagccgc cgcgccacgt cagcaaccgg tgcttctcgg ggcggccacg ggaacgccag
     8161 tactggaggc acatcttgat cgcgacctcc accgataccg agcccgaatc ggcgaggaag
     8221 acatgctgca gcccggcggg ggtgatgtcg accagcagtc gcgcgaggtc gacggccggc
     8281 ccgtgcgtga gaccaccgaa catcacgtgg ctcatcgagc cgagctggtc gcgcaccgcg
     8341 gcgtcgagca cggggtgggc gtagccgtgg atcgccgccc accacgacga catgccgtcg
     8401 accaactccc ggccgtcggc gagccgcagc cggacccccg cggccgactc gaccgcgagc
     8461 ggcgccacgc tcgccggcat cggtgcgtag ggatgccaca cgtgcgcgcg gtcggccgcg
     8521 agcaggtcgt cgatcgtcac gaccagggag cctaggcgga ggaggtgacg ggccgactaa
     8581 agtggcgggt catgtccacc gccgccgagt ccgcgaccgc gcatcccgcg ctgtcccggc
     8641 gccgggagct cggtgccacg agcgctcgtt ccctactgct caccgtgctc ggggagttcg
     8701 tgctgccgcg cgacgatgcg gtctggacgc aggtactcat cgacgtgctc ggtgggctcg
     8761 gggtcgagac gaagtcggcc cggcaggcac ttgctcgtac cgcggcggag ggcctgctcg
     8821 tctccgatcg ggcggggcgc cgtgtccggt gggcgctcag cgagcagggc cgcaggctgc
     8881 tctccgacgg cgccgcgcgc atctacgggt tcggggcagc ggcgcgaccg tgggacgggc
     8941 ggtggctggt gctgctcgtc agcgtgcccg aggcccgccg tcagctgcgg caccgcctgc
     9001 gcacgcgcct ggcctgggcc gggttgggct cccccgcccc cggtgtctgg ctcacccccg
     9061 accccaccaa acaggacgag gtggccgagg tggtcgccga cctcgagctg accggcgtga
     9121 gcagctcgtt cgtgggcccg ttcggggcga tcggcgccca gctgcaggtc gtcgagcagg
     9181 cgtggaacct cgacgaggtg gagaccgcgt acgaggagtt cctcgagacg ttcgcgtgtg
     9241 cgacgccgac cgacccggcc gacgtgctcg cccaccagat ccacctcgtg cacacctggc
     9301 gccgcttccc gttcctcgac ccgaagctac ccggcgagct gctgcccgcg ggttgggccg
     9361 gagcccgggc ggcggagctg ttcgacagtc ttcacagtcg ctgggacgag ccggcccagc
     9421 agcactggct ccggctcgtc ggcacggcca gctgaggcac gcacctcacc tcacgcacct
     9481 cacggatgct gcgcgcgtac accgctcggc gggtgacagt gcgccaactt ggtggtgcac
     9541 tgacagctcc tgcaaggagg gactgtggcc gcctacgccg tctttttcag ctacaccccc
     9601 caggcctggg agcgcatgat cacgaaaccg ggtgatcgcg ctggcgcggc ccgggcgatg
     9661 atcgagagcg caggcgggac gctcgagtct ctctatttca tgctcgggga ccgcgacggc
     9721 tttgcggtgg tgcatgtgcc aggcaacgag gacgtagcgg ccctctcgat cgccatcgcg
     9781 agcagcggcg ctttctcaca agtcgagacc cgcgcgctcg tggcgccaca ggacctcgcc
     9841 ggagtcctgg agaaggcggc tgccgcccgc ggcgcctaca cgccgccggg cgagtaggcg
     9901 gtcaccctgc cagatcgagt tcgtcgggat cgatcgcctc tacgaccgtc gcgtacgcga
     9961 ccgttgccgc agcgagggcg tcgcggagct gggcgagggc agcgtgaggc atcggtggtc
    10021 acccgcgctc aacatcgaca gaatcgaggg cgcgctgggc attggcccgc tcgcccgggc
    10081 cgcggccggt ggccatgccg acccggtcga tgcgcagcag ggtgccgtcg acgatgacga
    10141 acgccttgcc cgctgcggca gtcacggcct cgtgcagagc cggcgccatc gcggtaagca
    10201 cgtcgagagc ttcgcggatg tagcggaaca ccgtggtcgt gccgatggcg aacccggcgg
    10261 cgagatcggc gtaggtctca cccttgcgca ggtgcgcgag caccagcagg gcctgctcga
    10321 cacggtgatc cccgcagggt aggtcggcac ccgaagctcc tggcccagac gtgttgatct
    10381 cgtcaatcgc ccgtctacca ggagcttcac tcatctcagc gctgccacac cgcagcaggc
    10441 ctcagcggtt ccagccagat tggaacggct cagtaacgta agccactttg tatcacaaaa
    10501 ttgccacaaa cgaggcgctc ggctagcttt gtgtcatccg attgatacag gagctgctgt
    10561 ggagaccctc gccggggcct gggccctgat cctctgggcg gtgctggtga tgatcgtttc
    10621 ggtcatccga cgtcgccgct gcctccggga cgccgcgcgc ccgatccggc gtggtcggca
    10681 ggcggcgctc gtggcgggcg ccctcgtgat cgccgcggtt gcgccggccg tcggggcgtg
    10741 gcacggccgc ttcgcgttcg gcctactgct ggccggtccg atcttctgcc tggtgatggc
    10801 cgccgggtcg ctggccaccg agctcctggt ccgcccggtc gccgccccgc gcaggcgggc
    10861 gaccctcgcg gcccggcact tcccgcagta cgtcgaccgg ccgcagctga tcgccgtgtg
    10921 cgcgctgttc gcggtcgccg tcgggttcct cacgaccacc gcgttcttcg gcgacgccga
    10981 cgacaagggt cggcccggcc ggtggctgtt cctgcacaac ttcaaccccg atccccggtt
    11041 cggcggctcg atcatcctgt ggccgggcgc ctactacgcc ctgccgctgc tggccgcgct
    11101 cggcctcacc ctgctcatga cctccattgc cctgcgccgg atcgccgccc gaccgcgaac
    11161 ggtcaccgcg gacggggctg ccgacccggt ggtcgcggac gagcgggcgc ggcggcgctc
    11221 ggcgaccgtc gtcctggctg ccgccggggt cgcggccgcg gccgcgctgt gcggggccgg
    11281 gatggtcgcg gcttccggtc tcgccggcgc cgaggcgccc tggaacgtcc tcgggatgct
    11341 cctctgggtc gtggtcgccg ccgctgccgc ggtcggcgcc tggtcggcgg ccgtcctggt
    11401 catccctgac cgggcgcgcc ggccgcaccc ggcaccggct acggcgtgac cggcgacccg
    11461 gtcgtcgaga tcgacctcga cgacccgacg ccgccctacg cccagctgcg ccgcggcctc
    11521 gcggccctga tcgagcaagg gaccctggcc ggtgggcagc gcctcccgac cgtccgccag
    11581 ctcgccggcg acctcggcat cgccgccgga acggtcatgc gcgcctacaa ggagctggag
    11641 agcgccgggt acgtccgcgg cgaccgcagg cgcggcacgg tcgtcatccc ccgggagcgc
    11701 gatccggtgg acgtcgacgc cgccctgcgc gctctcgccg tcgcctactg ccaccgcgcg
    11761 gaagatgtcg gcgccacccc tgagcaaacg ctggccgcgg tgtcccgagt gctggccgac
    11821 gaccacgcgg ctgtggtcac ccggggcggg agcgcttccg ggtagaccac ggcggtcgtc
    11881 ccactcgcgt cagcccaggc aacccggacc gagcaacgcc ttgaggtcgc ccatcaggga
    11941 cggggagtgg gtgaccttga gcgcgtcatc cagcctcaga acggtggcgc gggtgccgtt
    12001 gaccagcgac aggtgcacct ccgaggtgcc cgggtggttg ctcagcacgt ccttcagctt
    12061 cgagacccgc tggggcgtgc acagctcggt gcgcatgctc accttcaccg gcgcgccggc
    12121 actcccgccc gcatgggtga ggtcaggcac cgccaggtcg ttgacgatca gcgagacgcg
    12181 gtcgtcgcgg cggttgaccc tggccttgac cagcacgatc gcgtcctctg cgacgtcgac
    12241 cccgacgacc tggtaacagc gcgggaagaa cagcacctcg atgccaccgg cgaggtcctc
    12301 cagctgcgcc gacgcccacg gctcgccgtt cttgttcacc cggcggttca cgttggcgag
    12361 gatgccgccg atggtgacct ggtggccatc ggggacggtg ccctcgagga tggtggagat
    12421 cgccatgtcg gacttggccg tgagcacctg ctcgacgccg tgcagcgggt ggcccgacac
    12481 atagagcccg agcatctccc gctcgacggc gagcttgtgc ttggcgtccc actcgtcggt
    12541 gggcaccttg acgtcgaaca cggagtcgag ctccgacggg ccggacccgt ccatggagcc
    12601 gaacaggtcg aactgcccca tggaggcggc cttcttcgtg ctcatcaccg cgtcgatcgc
    12661 atcggcgtgg acgagcagca ggcccttgcg cggatggccg agcgagtcga atgcacccgc
    12721 tttgatcagc gactcgatga ccttcttgtt gcagaccacc gcgtcgacct tgcgcaggaa
    12781 gtcggagaag tcggtgaatg cgcccttctc cttgcgcgcc gcgacgatcg cgtcgacgac
    12841 gttcacaccg acgttgcgga tgccgccgag tccgaagcgg atgtcgttgc cgaccggggc
    12901 gaagttgcgc accgagtcgt tgacgtcggg cgccagcacc gtgatgccca tccggcggca
    12961 ttccgccagg tagaccgcgg ccttgtcctt gtcgtcgcgg accgacgtga gcaccgcggc
    13021 catgtactcg gccgggtagt tggccttgag gtaggcggtc cagtaggaga ccacgccgta
    13081 cgccgcggag tgggcgcggt tgaacgcgta gtcggagaac ggcagcagga tgtcccagag
    13141 cgtcttgacc gcggcctcgg agtagccgtt gtccttcatg ccctgggcga agccggcgta
    13201 ctccttgtcg aggatctcct tcttcttctt gcccatcgcg cggcgcagca ggtcggcctt
    13261 gccgagcgag tacccggcca gcttctgcgc gatggccatg acctgttcct ggtacacgat
    13321 caggccgtag gtctcgccga ggatgtcctt gagcggctcc gccagctccg ggtggatcgg
    13381 agtgacgtcc tggcgcttgt tcttgcggtc ggcgtagtcg ttgtgcgcgt tggcgcccat
    13441 cggccccggg cggtacagcg cgccgacggc ggagatgtcg tcgaactccg tgggggccat
    13501 gcgccgcaaa aggtcgcgca tcggcccacc gtcgagctgg aacaccccga gggtgtcacc
    13561 gcggcccagc agctcgtagg tcgtcgggtc gtcgagctcc aggctctcga tcacgagcgc
    13621 cggcttgccg ttgagctgga tgttctccag ggcgtcgtcg atgatcgtga ggttgcgcag
    13681 gccgaggaag tccatcttga gcagcccgag cgtctcgcag gcgcccatgt cgaactgcgt
    13741 gatgatggcg ccgtcggagt cgcggcgctg gatggggatg acgtcgatca gcggcttgct
    13801 cgacatgatc acgccggccg cgtggacgcc ccactgccgc ttgagaccct cgaggccgcg
    13861 cgccgtgtcg atgatctcct tgacctgcgg gtcgctctcg tagagcgccc gcacctcggt
    13921 ggcctcggag tagcgcttgt gggacgggtc gaacaggccc gagagcggga tgtccttgcc
    13981 catcactgcg gccggcatcg ccttggagat gcggtcggcc acggcgtagc cgggctggcc
    14041 gaacagcacg cgcgccgagt ccttgatcgc ggccttcgcc ttgatcgtgg agtaggtgat
    14101 gatctgggcg atccggtcct cgccgtactt ctccgtggtg tagcggatca tctcgccgcg
    14161 ccgacgctcg tcgaagtcca tgtcgatgtc gggcatggag atgcggtcgg ggttgaggaa
    14221 ccgctcgaag atcagcttgt gcttgatggg gtcgaggtcg gtgatgccca gcgcgtacgc
    14281 cacgagcgag cccgcggccg acccgcggcc gggcccgcac cggatgccga cggacttggc
    14341 gtgctggatg aggtcggccg tgacgaggaa gtagcccggg aaccccatct ggatgatgac
    14401 gccgagctcg tattcgacct gcgtgcggta ctgctgcgtg atcccgttcg ggaaccgcat
    14461 gtgcatcccg cgctcgactt ccttgatcag ccagctctcc tccgtctcgc cctccgggac
    14521 gggagcgcgg ggcatgaggt cctggccctc ggtgaacccg acgtcgacgc gctcggcgat
    14581 gagcagcgtg ttgtcgcagg cctcggggaa caccggatcc cactgggcgc gcatctcggc
    14641 aggggacttg aggtagaagt cggaggcgtc gaacttgaag cggcccgggt cggcgagggt
    14701 cttaccggtc tgcacgcaca gcagcacctc gtgcgacttg gcgtcctccg cgtaggtgta
    14761 gtgcaggtcg ttggtggcga ggccggggag gtcgagcttc ttcttgaggt cgtagagcgc
    14821 gtcgcggacc tggcgctcga tcgcgatgcc gtggtcgagc agctcgcaga agaagttgtc
    14881 cttgccgaag atgtcgcggt agtcgctcgc cgcctggcag gcggcgtcga acttgtcctg
    14941 ctgcagcagc cgctgcacct caccggaggg gcacccggtg gtggcgatga tccccttgcc
    15001 gtaggtctcc agcagctcgc ggtccatgcg gggcttgtag tagtagccct cgagactggc
    15061 cagcgacgag agccggaaga ggttgtgcat cccctccgtg ttctccgcga gcaacgtcat
    15121 gtgggtgtac atctcacccg ggttgtcgtc gctgacggtc tgcccgccca gggtggcgcg
    15181 cttgcgctcg tgccggctgc cgggcgagag gtagccctcc atgccgatga tcggcttgac
    15241 cccgtgggct ttcgccttgc gccagaactc gtaggcgccg tagacgttgc cgtggtcggt
    15301 catcgccagc gccggcatct ccatgcgcgc ggcttccttg aacaggtcgt ccagtcgggc
    15361 cgcaccatcg agcatggaga actcggtgtg cgtgtggagg tggacgaagc tgtcgccgct
    15421 gctcggcctg gccatcgggg tggggagctc cctcctggcg cggcccgcga gggcagcgct
    15481 cgatcggcgc ggccgccgca acgggacatc ctcgggggac gcaccgggcc gactggccgc
    15541 tgggatgact caggctaacc ccgttccccg acagtcttcc gcacccgggc ggtccttcgg
    15601 ccgaacgcgc tgctcacctc gccgaccggc gtgtcgacag ccgcgcagca cggcgcgggc
    15661 agcccctcga cgaacgaacg gcgccctcgt ccagaagcat cggacgaggg tgccgttcgt
    15721 tgcatccagc gggccgccgc cgcccggcac gggagcccta gactcgcctg ccatgagccg
    15781 gccctccccg tacgcgtcgc tcgtcgcgcg tgtgcccgtg cgggaggacg aggtggtgct
    15841 gcgcggcggc cgcacccgcc ttcgggtcta cggcaaggac gacgccgcct atcggatggt
    15901 cttcctgcac gggctgcgcg gcgaccacca cgggctggag ccgatcgtcg cgcacctgcg
    15961 ggacgtgcgg gtggtcgtgc cggacctgcc cggcttcggg gagtccccac cgctcccgga
    16021 cgacacccac gacgtggccg gctacgcggc gtggacgcgc gagctgctcg cggccgtggc
    16081 cccggggggc gacgccgtgc tcgccggcca ctccttcggc tcgatcgtgg ctgccgcggc
    16141 ggtcagccac tgtggaccgg agccggtccc ctcgacgcgg gtgcgcggcc tcgtgctcgt
    16201 caacccgatc gcggcgtccg cgctggccgg gccgcgggtt gtcatgaccc agctggccgt
    16261 gctctaccac cggctcgccg ccgcccttcc cgagcgcgcc ggcaccgcac tgctgcgcaa
    16321 ccgggcgatg acgagggtgt ccagcgtggc gatggtcacg acggtcgacc cgcagctgcg
    16381 tcgctggatc cacggcgagc acgaccggta cttcagcacg ttcgccgacc ggcgcacgct
    16441 tctcgaggct tttcacgcct cggtgcgcca cgacgtcggc gagttcgccc acgccgtcac
    16501 cgtcccgacg ctgctggtgg ccgccgagcg cgacgacatc gcaccacctg cggcccagcg
    16561 cgcgctggcc ggccgcttcg cggacgtgcg gttggtgatg gtccccggca ccggccatct
    16621 cgcccactac gaggctcccg acgcggtcgc ggccgcgatg tcggcgttcc tcgccgcgct
    16681 gccggtcccg tgagaatcct gctcgactgc cgctatgtcc gcatcgac
//
LOCUS       DASRSC010000225         4185 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4996447,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000225 DASRSC010000000
VERSION     DASRSC010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4996447"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VFE39_11160"
     CDS             complement(<1..728)
                     /locus_tag="VFE39_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HET6259200.1"
                     /translation="MTSDTAVDQSSAQSDGGREVLKRDRVVIRFAGDSGDGMQLTGDR
                     FTSETAAFGNDLSTLPNFPAEIRAPAGTLPGVSSFQLHFANYDILTPGDRPDVLVAMN
                     PAALKANIADVPPGGVLIVNTDEFTKRNLTKVGYESNPIEDGSLEQYSVFPVAMATLT
                     RGALADTGLSKKDAERSKNMFALGLLSWMYHRPHESTERFLREKFARRPDLAEANILA
                     FKAGHAYGETTESFAVTYEVAPAHL"
     gene            complement(870..1625)
                     /locus_tag="VFE39_11165"
     CDS             complement(870..1625)
                     /locus_tag="VFE39_11165"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET6259201.1"
                     /translation="MATAVRTTITDRVAVLTVSNPERRNAMNLDLSAKLVDAVQSAAG
                     DDGVGAIVITGEEPAFCAGGDLSELAKADPATLRTVYAGFLAVADCPLPTIAAVNGAA
                     VGAGLNLALACDLRLAGPRAKFDARFLPLGLHPGGGYTWMAQRVLGPQGAAALTLFGD
                     VVDADEAARIGLVYRVCDDVVAAAVELGGRAAAAPPDLVRTTKATLRLTAGMATQAEA
                     VEVEVRAQAASVHSDEFQQRLAALQAKISRSSR"
     gene            1768..2631
                     /gene="htpX"
                     /locus_tag="VFE39_11170"
     CDS             1768..2631
                     /gene="htpX"
                     /locus_tag="VFE39_11170"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019814944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET6259202.1"
                     /translation="MHSSMNGLKTALLLGGLSALVLFVGSLFGRGGLLIALVVALGMN
                     GYAYFNSDKLALRAMHARPITEAEHPGMYRIVRELSRTARQPMPRLYISPTAAPNAFA
                     TGRSPRHAAVCATTGLLSILDERELRAVLGHELSHVYNRDILISSVAGALASMVSLLA
                     NIALFAGMFGGGDEDRPNPLALLLIALLGPVAAGLVQMAVSRSREYQADASGAALTRD
                     PLALASALRKLERGTQLAPLPPEPRLMAQSHLMIANPFRAGDQMTRLFSTHPPLQDRI
                     RRLEEMYRGGP"
     gene            complement(2933..3262)
                     /locus_tag="VFE39_11175"
     CDS             complement(2933..3262)
                     /locus_tag="VFE39_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6259203.1"
                     /translation="MTPVERFQKAFDARDVDAIMAAMTPDCVFEDTTPPDGRRHVGAE
                     AVRAAWTALFATSPDGVFTTEELIDAGDRVVARWRYEWGSGHVRGVDVFTVRDGLVAE
                     KLAYVKG"
     gene            complement(3259..3831)
                     /locus_tag="VFE39_11180"
     CDS             complement(3259..3831)
                     /locus_tag="VFE39_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HET6259204.1"
                     /translation="MLTAVDVREIYARCSAEFGAQVHAVDGRWDAPTPLPGWTVRDLV
                     HHLVEEELWAPPLFAGSTIEEIGSPFEGDLLGDDPLGTFDIAAARALGAVCAEGALDR
                     VVHLSFGDHPGREYAMQLAADHLVHAVDLARALGTDETMDAAAVVSVRGWFDTMESTY
                     REIGVIGPRADVPDGAGAQAELLAMMGRTP"
     gene            complement(3837..>4185)
                     /locus_tag="VFE39_11185"
     CDS             complement(3837..>4185)
                     /locus_tag="VFE39_11185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6259205.1"
                     /translation="ATPTETREFPNGRLDLLRVAGADIGRLTLQPGWRWSTDVKPAAG
                     TELCEAPHFQYHVSGTLHVSTADGEEFDAVAGQVTALPSGHDAWVVGDEPVVVVDWWG
                     ASDYARGASEQGG"
BASE COUNT          580 a         1514 c         1473 g          618 t
ORIGIN      
        1 agatgggcgg gcgcgacctc gtaggtgacg gcgaacgact cggtggtctc gccgtaggcg
       61 tgccctgcct tgaacgcgag gatgttggcc tcggcgagat ccggacgacg ggcgaacttc
      121 tcccgcagga accgctcggt gctctcgtgc ggccggtggt acatccacga cagcagcccg
      181 agggcgaaca tgttcttcga ccgctcggcg tccttcttgg acaggccggt gtccgcgagt
      241 gcgccccggg tgagggtggc catcgcgacg gggaacaccg agtactgctc gagcgagccg
      301 tcctcgatcg gattcgactc gtagccgacc ttcgtgaggt tccgcttggt gaactcgtcg
      361 gtgttgacga tcagcacccc gccggggggc acgtcggcga tgttggcctt gagtgcggcc
      421 gggttcatcg caaccaggac gtccgggcgg tcgcctgggg tgaggatgtc gtagttcgcg
      481 aagtgcagct ggaacgacga cacgccgggg agagtgccgg caggggcgcg gatctcggcc
      541 gggaagttgg gcagcgtgga gaggtcgttg ccgaacgccg cggtctcgga cgtgaaccgg
      601 tcgccggtga gctgcatgcc gtcgccggag tcgcccgcga agcggatgac gacgcggtcg
      661 cgcttgagca cctcacggcc gccgtcggac tgggccgagg actggtcgac ggcggtgtca
      721 gaagtcattc gaggtcggtc ctggggtcga cgggacgtgc tgagggttgc ctcaggaggt
      781 taactcccgg ttcgtccgag tgtcctagca ccccgctgca cgtcacacct gatcgttcgc
      841 tcagacgcaa ggccgtgtta cccgctttct cagcggcttg acctactgat cttcgcctgc
      901 agtgccgcaa gccgctgttg gaactcgtcc gagtgaaccg atgccgcctg ggcccgcacc
      961 tcgacctcga ccgcttcggc ctgggtggcc atcccggcgg tcaggcgcag cgtcgccttg
     1021 gtggtgcgga cgaggtcggg aggcgccgcg gcagcccggc cgccgagctc gacggcggcc
     1081 gccaccacgt cgtcgcacac gcggtacacc aacccgatcc gggcggcctc gtcggcgtcc
     1141 acgacgtcac cgaagagggt cagcgcggcc gcgccctgcg gaccgagcac tcgctgtgcc
     1201 atccacgtgt atcccccgcc gggatgcagg ccgagcggca ggaaccgtgc gtcgaacttc
     1261 gcgcgggggc cggcgagccg caggtcgcag gcgagcgcga ggttcagccc ggcccccacg
     1321 gcggcgccgt tcacggcggc gatggtgggc agcgggcagt cggcgacggc gaggaaccct
     1381 gcgtagacgg tgcgcagcgt cgcggggtcg gccttcgcga gctcgctcag gtcgccgccc
     1441 gcgcagaacg ccggctcctc gccggtgatc acgatcgcgc ccacgccgtc gtcgcccgcg
     1501 gcgctctgca cggcgtcgac gagcttcgcc gacagatcca ggttcatcgc gttgcgccgc
     1561 tcggggttcg acaccgtgag gacggcgacc cggtcggtga tcgtggtgcg gacagcggtg
     1621 gccatgtccg catcttgctc tgcgcggcgg ccgttcgcgt cgttgtgccc gtcacgtcgc
     1681 tccggcggat cgggcggcgt ggcccgatcg tccggaacac cacccgggcg tggttcgttg
     1741 tccgaacggg agcaggaagg acccgaagtg cacagttcga tgaacgggtt gaagacggcg
     1801 ctgctgctgg gtgggctgag cgcactggtg ctgttcgtcg ggtcgctctt cgggcgcggg
     1861 gggctgctga tcgccctcgt ggtcgcgctg ggcatgaacg gctacgcgta cttcaactcc
     1921 gacaagctcg cgctgcgcgc catgcacgcg cggccgatca ccgaggcgga acacccgggc
     1981 atgtaccgga tcgtgcgcga gctctcgcgc acggcgcgcc agcccatgcc gcggctctac
     2041 atcagcccga ccgcagcgcc caacgcgttc gccaccggtc gtagcccccg gcacgccgcc
     2101 gtctgcgcca ccaccggcct tctgtccatc ctcgatgagc gggagctgcg ggcggtgctc
     2161 gggcacgagc tgagccacgt ctacaaccgc gacatcctga tctcctcggt ggccggggcg
     2221 ctcgcctcga tggtgagcct cctcgccaac atcgccctgt tcgccgggat gttcggcggt
     2281 ggcgacgagg accgacccaa cccgctggcg ctgctgctga tcgcactgct cgggcccgtg
     2341 gcggccgggc tcgtccagat ggccgtgagc cggtcgcgcg agtaccaggc cgacgccagc
     2401 ggcgctgcgc tcacccgtga cccgctggcg ctcgcgtcgg ccctgcgcaa gctcgaacgc
     2461 ggcacccaac tcgcaccgct gcccccggaa ccgcggctga tggcccagtc gcacctgatg
     2521 atcgccaacc cgttccgggc aggcgaccag atgaccaggc tgttctccac gcacccgccg
     2581 ctgcaggacc gcatccggcg gctggaggag atgtaccggg gaggcccgtg agcggatacg
     2641 ggtgcccgcg cacccgtatc cactcacgac ctgcggtcag gcggggacgg ggaacagacg
     2701 cccaagccac gcctgccagt cggcggtcag cgcctcggcg tccaccggct ctccgcagaa
     2761 gtagtggtag gcgctcaccc cgcagcctgc ctcgccctcc gctccgatgc ggtgcaggcc
     2821 gcgcgccgac cggacgccga aaaccggagc acggtgccgc cgccgtcgcg cgatcgcctg
     2881 ccacgcctgc tccgggttgg cgtccagctc gctcctcccg atcgcgccgg cgtcagccct
     2941 tcacgtaggc gagcttctcg gccacgagcc catcccggac ggtgaagacg tcgacgccgc
     3001 ggacgtgccc ggacccccac tcgtagcgcc accgcgccac cacccggtcc ccggcatcga
     3061 tgagctcctc ggtggtgaac acaccgtccg gcgaggttgc gaacaacgcg gtccaggccg
     3121 cccgcaccgc ctcggccccg acgtgccggc gtccgtccgg tggggtcgtg tcctcgaaga
     3181 cgcagtcggg cgtcatcgcg gccatgatcg catcgacgtc ccgcgcgtcg aatgccttct
     3241 ggaagcgctc cacgggggtc atggcgtccg tcccatcatc gccagcagct cggcctgcgc
     3301 tccggcgccg tccgggacgt cggcccgcgg gccgatcacc ccgatctccc ggtaggtcga
     3361 ctccatcgtg tcgaaccagc cccgcaccga cacgacggcg gcggcgtcca tcgtctcgtc
     3421 ggttcccagc gcccgagcca gatcgacggc atgcacgagg tggtcggcgg cgagctgcat
     3481 cgcgtactcg cgaccggggt ggtcgccgaa ggacaggtgc accacccggt ccagtgcccc
     3541 ctcggcacac acggcgccga gggcccgtgc ggcggcgatg tcgaacgtgc ccagcgggtc
     3601 gtcgcccagc aggtcgccct cgaacggcga gccgatctcc tcgatcgtcg agccggcgaa
     3661 cagcgggggc gcccagagtt cttcctcgac gaggtggtgc accaggtccc gcacggtcca
     3721 ccccggaagc ggggtcggcg cgtcccagcg accgtcgact gcgtgcacct gcgccccgaa
     3781 ctcggcgctg cacctggcgt agatctcgcg gacgtccaca gcggtgagca tgcccgtcac
     3841 ccgccctgtt cagaagcgcc gcgggcgtag tccgacgcgc cccaccagtc gaccacgacg
     3901 accggctcgt cgccgaccac ccaggcgtcg tgcccggacg gcagtgcggt gacctgccct
     3961 gcgacggcgt cgaactcctc gccgtcggcg gtactgacgt gcagcgtgcc ggacacgtgg
     4021 tactggaagt gcggcgcctc gcacagctcg gtgcccgccg ccggcttgac gtcggtggac
     4081 caccgccagc ccggctgcag ggtcagccgg ccgatgtccg cgccggcgac ccgcagcagg
     4141 tcgaggcgcc cgttcgggaa ctcgcgggtc tcggtcgggg tggcg
//
LOCUS       DASRSC010000226         5680 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2870008,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000226 DASRSC010000000
VERSION     DASRSC010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2870008"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFE39_11190"
     CDS             <1..802
                     /locus_tag="VFE39_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259206.1"
                     /translation="PNGAPGQVHTAAAVHRLLTEVVAEHDRVALFPPADDGVPSDPGD
                     VWQDRGRAVTPPDPKRRRKLAIALTALAAAVLLVGGYIAIQVSSVFSEGNTPPIVVGG
                     APVAPPNVPPGGATPGQGGIAAVAGVEVYDTVGDRDNAGRVSRVIDGNITSGWSTFSY
                     KQQFPALKPGVGVMVSFATAVQLSELTIQSPSAGTVIQVRSAPTPDAPFNETALITQV
                     TLGDGSTPVSLAGSQPVTHVLLWITKLSGGGDNHVSEIDEVRFLRAGS"
     gene            863..1414
                     /gene="sigM"
                     /locus_tag="VFE39_11195"
     CDS             863..1414
                     /gene="sigM"
                     /locus_tag="VFE39_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigM"
                     /protein_id="HET6259207.1"
                     /translation="MCSPAPTDAELLAAHRAGDRHAFTELTARYRSRLWGLALRTLHH
                     PEDAADAVQETLVAAYRRAGSYRGDASVRTWLHRILLNTCIDRIRHERRRTTVPLPDR
                     DLQAGHSDLATELVTRLAVDAALAALPVDQRVAVVLVDVQGWPLVEVAELLEVPVGTV
                     KSRCARGRARLAVLLGHLREDHR"
     gene            1411..2187
                     /locus_tag="VFE39_11200"
     CDS             1411..2187
                     /locus_tag="VFE39_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259208.1"
                     /translation="MTAELPDLALLADLDAGLLDPARARQVRAAAAANPRSAAMLNAL
                     AGIRAELASVPTPPVPPEIAARWAAALEAEAASAGERSAGIHPVEAGPGPARTTGDPA
                     GVRNPARPSRGARPPHRAGSSGPPGVRPWPRRPLRAAGAALVLAALVIAGVLWPRSAP
                     LPPLDRVNLAAAARAAIGTTDVGELADPDRRAGCLMAVAPALPPAAPLLGGRRVELDS
                     REAVLLVLATGRLGTFHVAVVDPGCGPAGGTLLESAVIGG"
     gene            2284..3282
                     /gene="trxB"
                     /locus_tag="VFE39_11205"
     CDS             2284..3282
                     /gene="trxB"
                     /locus_tag="VFE39_11205"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET6259209.1"
                     /translation="MNTDEVRELIIIGSGPAGYTAAVYAARAQLQPLVFEGTQFGGAL
                     MTTTEVENYPGFKDGILGPELMEQMREQAVRFGAELRSEDVEQVSLEGDVKTVVANGV
                     TYRSRAVILAMGAAARYLHVPGEQLLLGRGVSSCATCDGFFFRDKDIAVIGGGDSAME
                     EATFLTRFAKSVTIVHRRDEFRASRIMLERAKSDPKMRWRTNVEVVEVLGENSVSGLK
                     LRDRTTGEDSVLDVSGMFVAIGHDPRSELVRDVLATDDAGYVKVDTPSTRTAIPGVFA
                     AGDLVDHTYRQAVTAAGSGCAAAIDAERWLADGPVPSELAGGGYGQVSEQVAAVTG"
     gene            3279..3650
                     /gene="trxA"
                     /locus_tag="VFE39_11210"
     CDS             3279..3650
                     /gene="trxA"
                     /locus_tag="VFE39_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET6259210.1"
                     /translation="MTRVSLALSDERRSQMANTVNVTDDSFDADVLKSNKTVVVDFWA
                     TWCGPCKMVAPVLDEIAGENKDKLTVAKLDIDANPSTARDYQVMSIPTMIVFQDGKPV
                     KQIVGAKPKAALLSDLADYLG"
     gene            3831..4988
                     /locus_tag="VFE39_11215"
     CDS             3831..4988
                     /locus_tag="VFE39_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HET6259211.1"
                     /translation="MQLLRRGDSGPAVVEIRSTLRRFDLLADGAGGPGDDRFDDSVEQ
                     AVRAFQQQRGLITDGIVGPATYRALREAGWSLGDRMLALMISAPMSGDDVVALQERLL
                     ELGYDPGRPGGVFDEQTEAALRKFQREYGLVPDGLCGPATLRSLRQLGRKVTGGRPHL
                     LREQELLRQAGPRLRGKRIVVDPGHGGPDRGIVAAGVAEADLMWDLARRLVGRMTATG
                     MDAMLSRQEDTCPAETERAAFANNAGADLVLSLHTDANHSMHAQGLATFHFGNGSGAT
                     STVGEALAGFIQRELLTRTAMHDCRTQGRTWEMLRLTRMPTVRVEIGYLSHIGDRMRL
                     LDPAFRDIVAEGILVAVKRLYLLGQDDQPTGTFTFSDVLAREMERGGAQAI"
     gene            complement(5056..>5680)
                     /locus_tag="VFE39_11220"
     CDS             complement(5056..>5680)
                     /locus_tag="VFE39_11220"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6259212.1"
                     /translation="NLDDLPKRCRRCVFWELSEHLGKQAADFGSTELEKEAWVSEVLL
                     EWGSCGRIVHVEGVPAGYVMYAPPSAVPRAAEMPTGPVSADAVLLTTMQVMPEFAGEG
                     LGRMLAQAVVKDLTRRGVKAIEVFGEQRPGTEPGCLVPADFLRGVGFKTIRPHPRYPR
                     LRMELRSAMTWKEDVEAALEQLLGTITIPMQTSGVAEKSYRQVLSST"
BASE COUNT          852 a         2066 c         1955 g          807 t
ORIGIN      
        1 cccgaacggc gccccagggc aggtccacac cgccgccgcg gttcaccggc tgctcaccga
       61 ggtggtggcc gagcacgacc gcgtggccct gttccccccg gccgacgacg gcgtcccgtc
      121 cgaccccggg gacgtctggc aggaccgggg ccgtgccgtc accccgcccg acccgaagcg
      181 caggcgcaag ctcgccatcg cgctcaccgc actcgctgcc gccgtgctcc tcgtcggcgg
      241 ctacatcgcg attcaggtca gctcggtgtt ctccgaaggg aacactcccc cgatcgtcgt
      301 cggcggtgcg cccgtcgcgc ccccgaacgt cccgccgggg ggggcgaccc caggacaggg
      361 cgggatcgcc gcagtcgcgg gcgtggaggt ctacgacacc gtgggcgacc gtgacaacgc
      421 gggccgggtg tcgcgcgtca tcgacggcaa catcacgagc gggtggagca ccttcagcta
      481 caagcagcag ttcccggcgc tcaagcccgg cgtcggggtg atggtgtcgt tcgccacggc
      541 cgtgcagctg tccgagctca cgatccagtc accgagcgcc ggcacggtga tccaggtgcg
      601 ctcggccccc accccggacg cccccttcaa cgagaccgcg ctgatcaccc aggtgaccct
      661 cggggacggc agcaccccgg tgtcgctcgc cgggagccag ccggtcacgc acgtcctgct
      721 gtggatcacg aagctcagcg gcgggggcga caaccacgtc agcgagatcg acgaggtccg
      781 tttcctccgc gccggctcct gatctgcacc atttttccga aacgcccacg gcaggcgggg
      841 agcgccacct actctccgcc tcgtgtgctc cccggccccg accgatgcgg agctgctggc
      901 cgcgcaccgg gcgggtgacc gccacgcgtt cacggagcta acggcgcgtt atcgctcccg
      961 cctgtggggg ttggcgcttc gcacactgca ccacccggag gacgccgctg acgccgtgca
     1021 ggagaccctc gtggccgcgt accggcgggc cggctcgtac cggggcgacg cttcggtccg
     1081 tacctggctg caccggatcc tcctcaacac ctgcatcgac cgcatccggc acgagcgcag
     1141 gcgcacaacg gtcccactgc cggaccgcga cctgcaggcc gggcactccg acctcgccac
     1201 cgaactggtc acccggctgg ccgtcgacgc ggcgctcgcg gcactgcccg tcgaccagcg
     1261 ggtggcggtg gtgctggtgg acgtccaggg ttggcccctg gtggaggtcg ccgaacttct
     1321 ggaggtaccg gtgggcacgg tgaagagccg ctgcgcgcgg ggacgcgctc gtctggcggt
     1381 gctgctagga cacctgcggg aggaccaccg atgacggcag aactccccga cctcgccctg
     1441 ctcgcggacc tcgacgccgg gctgctcgat cccgcccggg cgaggcaggt acgtgcggcg
     1501 gccgccgcga atccccggtc ggccgcgatg ctgaatgcgc tcgcgggcat ccgcgccgag
     1561 ctggcctctg tgcccacccc gcccgtgccg ccggagatcg ccgctcgttg ggcagcggcc
     1621 ctggaggcgg aggcggcgtc tgccggcgaa cgctctgccg gcatccaccc tgtcgaagcc
     1681 ggacccggcc cggccagaac caccggtgat ccggcaggcg tacgcaaccc ggcgcggccg
     1741 agccggggcg cgcggccacc gcaccgtgca ggctcctccg ggccgccggg tgtccggccg
     1801 tggccgcgca ggccactgcg cgccgccggc gccgcactcg tgctcgccgc actcgtcatc
     1861 gccggcgtgc tgtggccccg gtcagccccc ctgccgccgc tcgatcgggt gaacctggcc
     1921 gcagccgcac gggcggcgat cggcaccacc gacgtcggcg agctggcaga tcccgaccga
     1981 cgcgccgggt gcctcatggc cgtcgctccg gccctaccgc ccgctgcgcc gctcctcgga
     2041 ggacgccggg tcgagctcga cagccgcgag gcggtactgc tcgtgctcgc cacagggcgc
     2101 ttgggcacgt tccacgtcgc ggtcgtcgac cccggctgcg ggcctgccgg cggcaccctc
     2161 ctggagtcgg ctgtcatcgg cgggtgatgt cactcacctc gaatcgggcg cccggtagca
     2221 gtcggaatgc ccgtccctgg gatcgtgttg acaggcaaag acgacagggg aggacgggcg
     2281 ccagtgaaca ccgacgaggt gcgcgagctc atcatcatcg ggtcgggtcc ggccgggtac
     2341 accgcggccg tatacgcggc ccgcgcacag ctgcaaccgc tggtattcga gggcacgcag
     2401 ttcggcggcg ccctgatgac caccacggag gtcgagaact accccggctt caaggacggg
     2461 atcctcggcc cggagctcat ggagcagatg cgcgagcagg ccgtgcggtt cggtgcggag
     2521 ctgcgctccg aggacgtcga gcaggtgtcg ctggagggcg atgtcaagac cgtcgtcgcg
     2581 aacggcgtca cctaccgctc ccgggccgtg atcctggcga tgggtgcggc cgctcggtac
     2641 ctgcacgtcc ccggtgagca gctgttgctg ggacgggggg tcagctcctg tgccacgtgc
     2701 gacggcttct tcttccgcga caaggacatc gccgtgatcg gcggcggtga ctcggcgatg
     2761 gaggaggcca ccttcctcac ccggttcgcc aagtcggtga cgatcgtgca ccgacgtgac
     2821 gagttccggg cctcccggat catgctggag cgcgcgaaga gcgaccccaa gatgcgctgg
     2881 cgcaccaacg tcgaggtcgt cgaggtcctc ggcgagaaca gcgtgtccgg cctgaagctg
     2941 cgcgaccgca ccaccggcga ggactccgtg ctcgacgtct ccgggatgtt cgtcgcgatc
     3001 ggccacgacc cgcgcagcga gctcgtgcgc gacgtgctcg ccaccgacga cgccggctac
     3061 gtgaaggtcg acacgcccag cacgcgcacc gcgatccccg gcgtgttcgc cgcgggtgac
     3121 ctcgtcgacc acacctaccg gcaggccgtc actgcggcgg gctcgggctg cgcggccgcg
     3181 atcgatgccg agcgttggct cgccgacggc cccgtaccca gcgagctcgc cggcgggggc
     3241 tacgggcagg tgtcggagca ggtcgccgcc gtcaccggct gactcgggtt tcactagctc
     3301 tttccgacga acggagatca caaatggcca acaccgtcaa cgtcaccgac gacagtttcg
     3361 acgcggacgt cctcaagagc aacaaaacgg tcgtcgtcga cttctgggcg acgtggtgcg
     3421 gtccgtgcaa gatggttgcc ccggtcctcg atgagatcgc gggtgagaac aaggacaagc
     3481 tgacggtcgc caagctggac atcgacgcca atccgtcgac ggctcgcgac taccaggtga
     3541 tgtcgattcc caccatgatc gtcttccagg acggcaagcc ggtcaagcag atcgtcggcg
     3601 ccaagcccaa ggccgcgctg ctctccgatc tcgccgacta cctcggctga accgggcgag
     3661 ccatcccaca caagtacccc gcttccggat atcggacgcg gggtacttgt ctatcacgac
     3721 acaagcatgg tccgacacgg cccgccgtcc cccttctgtg aagcagcagg gcacaatgag
     3781 cgacggccca gcaccgcagc ggcggtgcgc gacgaacgag cgaggagtgc atgcagctgc
     3841 tgcgccgagg tgacagcggt ccggccgtcg tcgagatccg gtccacgctg cgacggttcg
     3901 acctgctcgc cgacggtgcg ggcggccccg gggacgatcg cttcgacgac tccgtcgagc
     3961 aggccgtacg cgcgttccag caacaacggg gcctgatcac cgacgggatc gtcgggccgg
     4021 ccacctaccg cgcgctgcgt gaggccggat ggagcctcgg cgaccggatg ctcgccctga
     4081 tgatctccgc accgatgagc ggggacgacg tcgtcgctct ccaggagcgg ctgctcgagc
     4141 tcgggtacga ccccgggcgc cccggcggcg tgttcgacga gcagaccgag gccgctctgc
     4201 ggaagttcca acgcgagtac gggctcgtgc ccgacgggct gtgcgggccg gccaccttgc
     4261 gctccttgcg tcagctcggt cgcaaggtca ccggggggcg cccacacctg ctccgcgaac
     4321 aggagctcct gcgccaggcc gggccgcggc tgcggggcaa gcggatcgtg gtcgaccccg
     4381 gccacggcgg tcccgaccgg ggcatcgttg ccgccggcgt cgcggaggcc gacctgatgt
     4441 gggacctcgc ccggcggctc gtaggccgga tgactgccac gggcatggac gcgatgctgt
     4501 cgcgccagga ggacacctgc ccggccgaga ccgagcgcgc ggcgttcgcc aacaacgcag
     4561 gcgccgatct cgtgctgtcg ctgcacaccg acgccaacca cagcatgcat gcgcagggcc
     4621 tggcgacgtt ccacttcggc aacggttcgg gcgccacgtc cacggtcggt gaagctctgg
     4681 ccggattcat ccagcgcgag ctgctcacgc gcaccgcgat gcacgactgc cgcacgcagg
     4741 gccgcacctg ggagatgctg cggttgacgc ggatgcccac tgtccgcgtg gagatcggct
     4801 acctctccca catcggtgac cgcatgcggc tgctggaccc cgccttccgg gacatcgtcg
     4861 ccgaaggcat cctcgtcgcc gtcaagcggc tgtacctgct gggtcaggac gatcaaccga
     4921 ccggcacgtt cacattctcc gatgtactcg cccgtgagat ggagcgtggc ggggcacagg
     4981 ccatctgacc ggcgtggtcg ccgtaggccc gcagcgtctc gtacagggcg cccggaacgg
     5041 tccggacagc gccgttcagg tgctgcttag tacctggcgg taggacttct cggcgacgcc
     5101 gctggtctgc atcgggatag tgatggtgcc gagcagctgt tccaacgcgg cctcgacgtc
     5161 ctccttccag gtcatggcgg agcgcagctc catccgcagc cgcggatacc tcgggtgcgg
     5221 ccggatggtc ttgaatccga cgccccggag gaagtcggcg ggaacgaggc agccgggctc
     5281 ggtgccggga cgctgctccc cgaacacctc gatcgccttg acgccgcgac gggtgaggtc
     5341 tttcacgacg gcctgcgcca gcatccggcc caatccctcg ccggcgaact ccggcatcac
     5401 ctgcatggtg gtgagcagca ccgcatcggc actgaccgga ccggtgggca tctccgcggc
     5461 gcgcggcacg gccgaggggg gcgcgtacat cacgtagccg gcggggactc cctccacgtg
     5521 cacgatccgg ccgcacgaac cccactcgag gagcacctcg gagacccagg cctccttctc
     5581 gagctcggtg gacccgaagt ccgccgcctg cttgcccaga tgctcggaca actcccagaa
     5641 cacacaccga cgacagcgct tgggcaaatc gtccagattg
//
LOCUS       DASRSC010000227         6918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_83944,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000227 DASRSC010000000
VERSION     DASRSC010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83944"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="VFE39_11225"
     CDS             complement(<1..816)
                     /locus_tag="VFE39_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6259213.1"
                     /translation="MNAPPAALLDARYQVGPVLARGGMSTVYRGTDIRLDRPVAIKVM
                     EPGLAADPAFRTRFEREARSAARIDHPAVVDVHDQGDHMGVDGPVLFLVMELVEGGTL
                     RDVLRARGALGVPAAVAVMEPVLAGLSAAHRLGLVHRDVKPENVLISRTGEVKVADFG
                     LVTAAAHAGASHAGMIMGTVAYLSPEQVATGAADARSDVYAAGVLLYELLTGSAPYTG
                     DTAISVAYRHVNSDMPAPSLIAGDVPPELDDLVLRATRRDPAARPADAAAMLAE"
     gene            884..1240
                     /locus_tag="VFE39_11230"
     CDS             884..1240
                     /locus_tag="VFE39_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2175c family DNA-binding protein"
                     /protein_id="HET6259214.1"
                     /translation="MNDVFVLSDVVATLPVAEVAERLNQPVSRVHQLVRDGHLLSFRR
                     DGEVVVPADFLGEDDAVVKGLYGTITVLRDGGYSDVDILRWLFAEDDSLPGTPVASLR
                     AGRHREVKRRAQAMAF"
     gene            complement(1270..2211)
                     /locus_tag="VFE39_11235"
     CDS             complement(1270..2211)
                     /locus_tag="VFE39_11235"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HET6259215.1"
                     /translation="MPGQGRAELDAAGITDPALRAAYVNCRELNARHGRTYFLATRLL
                     PRERRPAVHALYGFARHADEIVDDLGDHRPVAEKAARLDALSAQLERTLAGHPTDSAV
                     LTALADTAGRYGIDGRHFTDFMASMRMDTHVTGYATFDDLGVYVHGSAAVIGLQMLPI
                     LGTTVPRAEAEPPAAALGVAFQITNFLRDVGEDLDRGRLYLPAEELAAFGVDRDLLTW
                     CRRTRRADRRVRRALAHLVAHTRAVYRRAAPGITMLEPVSRACVACAFNLYCGILDEI
                     EAADYHVLHRRVRVSNRTRAAVALPGMLRAVVARARS"
     gene            complement(2189..3901)
                     /gene="mptB"
                     /locus_tag="VFE39_11240"
     CDS             complement(2189..3901)
                     /gene="mptB"
                     /locus_tag="VFE39_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HET6259216.1"
                     /translation="MKPPSPVDGSALPEALPGAASTEHASAPVSASAEAGPAPGEESP
                     HPAQPADSGGSASGSLWRTRLGNPPRRPLLIGLAGALMMVVGGFGAGGVLIRDPLLTN
                     SALGFWRYGHGREIATVLIYTGVALMVWAWVQLGREVLARRVGGRAVLTTAAVWTAPT
                     LLAPPLFTRDIFSYLAQGGLSLAGFDPYAVGPEAMPGIFTDNVHYFWQDTPAPYGPLF
                     ILIAKAVAWATGNNIILGVILMRLALLPGLLLLVWALPELTRRLGGRVPVALWIAVAN
                     PVMIIHMVGGGHNDLLVVGLLAAGALVALRGRPAGGIALVTAAMAVKASAGIALPFLV
                     LVWAARFPGSRRARIAKATAAGVGVFAVVFGLCTLVARVNLGWLPALSAPSMIVNWMS
                     IPTGVGEFLHTLVGLVANVPKQPFINVARIIGAALLLWIAARQWWAARDGGPDAVHRA
                     GIVLLAVAVLSPATLPWYMSWGMALLAMVAWSPRGLAWIVFASLMLVIVYYPNGEDAL
                     YNWPYLAACALLAALAAVSLLRPDPLRLAAGARARKPAEAMPMPATASVAAPSSPGAG
                     PGTG"
     gene            complement(3930..5483)
                     /gene="crtI"
                     /locus_tag="VFE39_11245"
     CDS             complement(3930..5483)
                     /gene="crtI"
                     /locus_tag="VFE39_11245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016166 - phytoene dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HET6259217.1"
                     /translation="MTRTVPGPTDHVVVVGAGLSGLSAALHLLGAGRRVTLIERAEHP
                     GGRAGRLDLHTERGIYQVDTGPTVLTMPDLLDEAFGAVGEKLEERLDLVELDPAYRTH
                     FADGSTIDVHTDGVAMEAEVRRVCGPAAAAGYRRTREWLTRLYRAEIDSFIGANLDSP
                     LDLIGPDLVRLAALGGFGRLGPRVARLLPDERLQRIFSFQALYAGVPPHLALGAYGVI
                     AYMDTIGGVFFPMGGMRQVGQALADAAVAAGAEIWYGRTATGLARSGGRVTAVRHADA
                     NVPGSDTERLVCDAVVLTPDLPVVRRLVGRSPRRAVPLRHSPSAVVLHAGLTGTRPEL
                     AHHTISFGSAWRRTFREIIDEGRLMSDPSLLVTRPTATDPGLAPAGSDLLFVLAPCPN
                     TDRGTLDWARIGPAYRDELLGVLEARDHPGLGGLTGDIEVSRLVTPSDWAADGLAAGT
                     PFSLAHTLFQTGPFRPRNLVRGLENVVLAGCGTTPGVGIPPVLISGRLAAQRITGPAA
                     GARRVPTGG"
     gene            complement(5480..6607)
                     /locus_tag="VFE39_11250"
     CDS             complement(5480..6607)
                     /locus_tag="VFE39_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET6259218.1"
                     /translation="MTHAEAVDTATPGYHRPDDGGLTAAVETELATFLRARTADSRAI
                     DPAFAEATDALVAFVLNGGKRIRPTFAWWGWRGAGGAVDAAESVAVLRAISALELIQA
                     CALIHDDLMDASATRRGRPTVHVEFARRHVAAGWGGQPARFGAAAAILLGDLALAWAD
                     DMLRAAGLPPPALVRAAPPWQAMRTEMLGGQYLDVLHQASGDTSARAALQIDRYKTAA
                     YTVERPLHLGAAIADAGPELIAAYRRFGADIGVAFQLRDDLLGVFGDPAVTGKPAGDD
                     LREGKRTLLLAFAVERAEEQGRTTAREAITAAVGDPDLDAAGVARIRDLLTELGAVRS
                     MEHRIAALTGSALDALSAVEIDEPAASELVQLATLATRRRS"
     gene            6749..>6918
                     /locus_tag="VFE39_11255"
     CDS             6749..>6918
                     /locus_tag="VFE39_11255"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HET6259219.1"
                     /translation="MKVTQRISGDRPVFSVEFFPPKDEVGEAELWRAVRRLEPLDPAF
                     VSVTYGAGGSSRR"
BASE COUNT          976 a         2413 c         2661 g          868 t
ORIGIN      
        1 ctccgcgagc atcgccgcgg cgtccgccgg acgtgcggcg gggtcgcgcc gggtggcgcg
       61 cagcacgagg tcgtcgagct ccggaggcac gtccccggcg atcagcgacg gcgccggcat
      121 atcggagttg acatgccggt acgccacgga gatggcggtg tcgccggtgt agggcgccga
      181 gccggtgagc agctcgtaga gcagcacgcc agccgcgtag acgtcgctgc gggcgtccgc
      241 cgcacccgtc gcgacctgct ccggggagag gtaggcgacg gtccccatga tcatgccggc
      301 gtgactggcc ccggcgtgcg ccgcagcggt gacgagtccg aagtcggcga ccttgacctc
      361 accggtgcgg ctgatcagca cgttctccgg cttgacgtcg cggtgtacga ggcccaggcg
      421 gtgtgccgcg gacaggccgg ccagcaccgg ctccatgacg gccaccgcag ccggcacacc
      481 cagtgcgccc cgggcgcgga ggacgtcgcg cagcgtgccg ccctcgacga gctccatcac
      541 caggaacaac accggaccgt cgacgcccat gtggtcgccc tggtcgtgca cgtcgacgac
      601 ggccgggtgg tcgatccgcg cggcggaacg ggcctcgcgt tcgaaccgcg tgcggaacgc
      661 ggggtcggct gcgagcccag gctccatcac cttgatcgcg accggccggt ccagccggat
      721 gtcggtgccc cggtagacgg tcgacatgcc gccgcgggcg agcacgggcc caacctgata
      781 gcgcgcgtcg agcagcgcgg ccggcggggc gttcacgggc aagacggtac cgccccaccc
      841 ggatggtgcc ggagcagccg ctgctgtggc atcgtgtttc ctcgtgaacg acgtgttcgt
      901 gctctccgat gtcgtggcga cgctgcccgt cgcggaggtc gccgagagac tcaaccagcc
      961 cgtctcacgg gtacatcaac tggtacgcga cggccacctg ctgtcgttcc ggcgcgacgg
     1021 tgaggttgtg gtccccgccg acttcctcgg cgaggacgac gcggtcgtca aggggctgta
     1081 cggcacgatc accgtgctgc gcgacggcgg ctactccgac gtcgacatcc tgcgctggct
     1141 gttcgccgag gacgactcgc tgcccggcac gccggtggcg tcgctgcgtg ccggacggca
     1201 ccgggaggtc aagcgccgcg cccaggcgat ggcgttctga ccctcgggca cgtgccggcc
     1261 gtgtgaagct caggagcggg cacgggcgac gacggcgcgc agcatccccg gcagggcgac
     1321 ggcggcgcgc gtgcggttcg acaccctcac cctgcggtgg agcacgtggt agtcggccgc
     1381 ctcgatctcg tcgaggatcc cgcagtagag gttgaacgcg caggcgacgc aggcccggga
     1441 caccggttcg agcatggtga tgcccggcgc ggcgcggcgg tagaccgcgc gggtgtgtgc
     1501 gacgaggtgt gcgagtgccc gccggacccg ccggtcggcc ctgcgggtcc gccgacacca
     1561 ggtgagcagg tcgcggtcga ccccgaacgc ggcgagctcc tcggcgggca ggtagaggcg
     1621 gccgcggtcg aggtcttctc cgacgtcgcg gaggaagttc gtgatctgga aggcgacgcc
     1681 gagggccgcg gcgggcggct cggcctcggc acgcggcacg gtggtgccca agatcggcag
     1741 catctgcagt ccgatcaccg cggccgagcc gtgcacgtag acgccgaggt cgtcgaaggt
     1801 ggcgtagccg gtgacgtggg tgtccatccg catcgaggcc atgaagtccg tgaaatggcg
     1861 gccgtcgatg ccgtagcgcc cggcggtgtc ggcgagggcg gtgagcacgg cgctgtcggt
     1921 cgggtgaccg gcgagggtgc gctccagctg cgcggacagc gcgtcgagcc gggcggcctt
     1981 ctccgcgacg ggccggtggt caccgagatc gtcgacgatc tcgtcggcgt gccgggcgaa
     2041 gccgtacagg gcgtgcacgg caggccgccg ctcgcggggt agcagccggg tggccaggaa
     2101 gtaggtgcgg ccgtgccggg cgttcagctc ccggcagttg acgtaggcgg ctcgcaacgc
     2161 cgggtcggtg atcccggcgg cgtcgagctc agcccgtccc tggcccggca ccgggggagg
     2221 acggcgctgc aacggacgca gtggccggca tcggcatcgc ctcggccggt ttgcgggccc
     2281 gggccccggc ggcgagccgg agtgggtcgg gccggagcaa cgacaccgcg gcgagggcgg
     2341 ccagcaacgc gcaggccgcg agatagggcc agttgtagag ggcgtcctcg ccgttggggt
     2401 agtacacgat cacgagcatc agcgacgcga agacgatcca ggcgagcccc cggggcgacc
     2461 aggcgaccat cgcgagcaga gccatgcccc agctcatgta ccacgggagg gtggcgggcg
     2521 acaggaccgc aacggccagc agcacgatcc cggcccggtg cacggcgtcc ggtccgccgt
     2581 cccgtgccgc ccaccactgc cgagcggcga tccagagcag cagggccgcc ccgatgatgc
     2641 gggcgacgtt gatgaacggc tgcttcggga cgttcgccac cagcccgacg agggtgtgga
     2701 ggaactcgcc cacgccggtg gggatcgaca tccagttcac gatcatcgac ggggcgctca
     2761 gcgcaggcag ccacccgagg ttgacccggg ccaccagcgt gcacagcccg aacaccacgg
     2821 cgaacacgcc gaccccggcc gcggtcgcct tcgcgatgcg tgccctgcgg gagcccggga
     2881 agcgtgccgc ccacaccagc acgaggaatg gcagcgcgat gccggcgctc gccttcacgg
     2941 ccatggctgc ggtgacgagc gcgatccccc cggcaggccg cccgcgcagc gcgaccaggg
     3001 ccccggcggc gagcagcccg acgacgagca ggtcgttgtg cccgccgccg accatgtgga
     3061 tgatcatcac ggggttggcg acggcgatcc acagggcgac gggtacccgc ccgccgaggc
     3121 gacgggtgag ctcgggcagt gcccagacca gcagcagcag ccccggcagc agcgcgagcc
     3181 gcatgaggat gacgccgagg atgatgttgt tgccggtggc ccaggccacc gccttcgcga
     3241 tgaggatgaa caggggcccg tagggcgcgg gggtgtcctg ccagaagtag tgcacgttgt
     3301 cggtgaagat gcccggcatc gcctccgggc ccaccgcgta cgggtcgaac ccggcgagcg
     3361 agaggccgcc ctgcgcgagg tagctgaaga tgtcgcgggt gaacagcggg ggcgccagca
     3421 gcgtgggcgc ggtccagacc gccgcggtcg tgagcacggc ccggccgccc acgcggcgcg
     3481 cgaggacctc gcggcccagc tggacccacg cccacaccat cagcgccacg cccgtgtaga
     3541 tgagcaccgt ggcgatctcg cgtccgtgcc cgtagcgcca gaagccgagc gcggagttgg
     3601 tgagcagcgg gtcgcggatc agcaccccgc ccgccccgaa cccgccgacg accatcatca
     3661 gcgccccggc cagcccgatc agcagcggtc gccgcggcgg gttgcccagt cgggtgcgcc
     3721 acaacgagcc ggacgcggac ccgcccgagt cggccggctg ggccgggtgc ggcgactctt
     3781 caccgggcgc cgggccggcc tctgccgacg ctgaaactgg cgccgatgcg tgctccgtcg
     3841 acgctgctcc tggcagcgcc tcgggcagcg ccgatccgtc gaccggtgat ggtggcttca
     3901 tgacgcgatc catgctcgca tgacccgtgt cagccgcctg tgggtacccg ccgcgccccc
     3961 gcggcgggcc ctgtgatgcg ttgtgcggcg agccgtccgg agatcagcac cggcggaatg
     4021 cctacgccgg gggtggtgcc gcagccggcc agcacgacgt tctccaggcc gcggacgagg
     4081 ttccgcggcc ggaacggtcc ggtctggaac agggtgtgcg cgagggagaa cggggtgccc
     4141 gcggcgagcc cgtcggcggc ccagtcggac ggcgtcacca ggcgggacac ctcgatgtcc
     4201 ccggtcagtc cgcccaggcc ggggtggtcg cgggcctcga ggacgcccag cagctcgtcg
     4261 cggtacgcag ggccgatgcg ggcccagtcc agcgtgccgc ggtcggtgtt ggggcacggg
     4321 gcgagcacga agaggaggtc ggagcccgca ggggcgagcc ccgggtcggt cgcggtcggg
     4381 cgcgtgacca gcagcgaagg gtcgctcatc aggcggccct cgtcgatgat ctcgcggaag
     4441 gtgcgccgcc acgccgaccc gaaggagatc gtgtggtgcg cgagctcggg ccgcgtgccg
     4501 gtgagtccgg cgtggagcac aaccgcgctg ggggagtggc gcaggggcac ggcgcggcgc
     4561 gggctgcggc cgacgagccg gcgcacgacc ggaagatccg gtgtcagcac gaccgcgtcg
     4621 cagacgagcc gttcggtgtc gctacccggg acgttcgcgt cggcatgccg gacggcggtg
     4681 acccgcccgc cgctgcgcgc caggccggtg gcggtgcgcc cgtaccagat ctctgcgcct
     4741 gcggcgaccg cagcgtcggc gagcgcctgc cccacctgcc gcatgccgcc catcgggaag
     4801 aagacgccac cgatcgtgtc catgtaggcg atgacgccat acgcgccgag agcgaggtgc
     4861 ggtggcacac ctgcgtacag cgcctggaac gaaaagatcc gctgcagccg ctcgtcgggg
     4921 agcagccgcg ccacccgcgg gccgagccgt ccgaatccgc ccagcgccgc gagccggacg
     4981 aggtcggggc cgatcaggtc gagcggtgag tccaggttgg cgccgatgaa ggagtcgatc
     5041 tcggcgcggt agagccgcgt gagccactcg cgcgtacgcc ggtagcccgc cgcggccgcg
     5101 ggaccgcaca cccgccgaac ctcggcctcc atcgccacgc catccgtgtg gacgtcgatg
     5161 gtggagccgt cggcgaagtg ggtgcggtag gcggggtcga gctcgacgag gtcgaggcgc
     5221 tcctccagct tctcgcccac ggcgccgaac gcctcgtcga gcaggtccgg catggtgagc
     5281 acggtgggtc cggtgtccac ctggtagatg ccgcgctccg tgtgcaggtc gagccgtcct
     5341 gcgcgccctc cgggatgctc tgcgcgctcg atgagcgtga cgcggcggcc ggcccccagc
     5401 aggtgcaggg cggcgctcag tccggacagg cccgcgccga ccacgacgac gtgatcggtc
     5461 ggtccgggga ccgtgcgggt caactcctcc tccgggtggc cagtgtggcc agctggacca
     5521 gctcggatgc tgccggctcg tcgatctcga cggccgacag agcatcgagc gcggaaccgg
     5581 tgagggccgc gatccggtgc tccatcgacc ggacggcccc gagttcggtg aggaggtcgc
     5641 ggatccgggc caccccggcg gcgtcgaggt ccggatcacc gacagcggcg gtgatcgcct
     5701 ctcgcgccgt cgtgcgcccc tgctcctccg cccgctcgac ggcgaacgcc agcagcagcg
     5761 tgcgcttgcc ctcgcgcagg tcgtcgccgg caggcttgcc ggtgacggcc gggtcgccga
     5821 agacgcccag caggtcgtcg cggagctgga acgccacgcc gatgtcggcc ccgaagcgcc
     5881 ggtacgcggc gatcagctcc ggcccggcat cggcgatcgc cgcaccgagg tgcaacgggc
     5941 gttccacggt gtaggcggcg gtcttgtagc ggtcgatctg caacgcagcg cgcgccgagg
     6001 tgtcgccgct ggcctggtgc agcacatcga ggtactgccc gccgagcatc tccgtgcgca
     6061 tcgcctgcca gggcggtgcc gccctgacga gcgcgggtgg tgggaggccc gccgcgcgca
     6121 gcatgtcatc ggcccacgcc aacgccaggt cgccgagcag gatggccgcg gccgctccga
     6181 accgggcggg ctgcccgccc catccggccg cgacgtgcct gcgggcgaac tcgacgtgca
     6241 ccgtcgggcg gccccgccgg gtggcggagg cgtccatcag gtcgtcgtgg atcagcgcgc
     6301 aggcctggat cagctcgagc gcgctgatcg cgcgcagcac ggccaccgac tcggccgcgt
     6361 cgaccgctcc acccgcgccc cgccagcccc accacgcgaa cgtgggccgg atacgcttcc
     6421 ccccgttgag gacgaacgcg acgagcgcat cggtggcctc ggcgaacgcc gggtcgatgg
     6481 ctctcgagtc ggccgtgcgg gcccgcagaa aagtggcgag ctccgtttcg acggcggcgg
     6541 tgaggccgcc gtcgtcgggt cggtggtagc cgggtgtcgc ggtgtcgacc gcttccgcgt
     6601 gagtcatcgg gagcaccgcg tcgggtcggg ccggagggcg ccgcggcatc ggggaagcag
     6661 atgtgctggt gagcggggca cgggtggagg gtagcggcgg gggcacactc cggcgcgctg
     6721 acggcacggc cggtcgtagg ctgaacccgt gaaggtcacc caacggatca gcggcgaccg
     6781 gcccgtgttt tcggtggagt tctttccgcc caaggacgag gtgggcgagg cggagctgtg
     6841 gcgggcggtg cgccggctgg agccgctcga cccggcgttc gtctcggtga cgtacggcgc
     6901 gggcggctcc agccggcg
//
LOCUS       DASRSC010000228         3470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1069717,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000228 DASRSC010000000
VERSION     DASRSC010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1069717"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..900)
                     /locus_tag="VFE39_11260"
     CDS             complement(46..900)
                     /locus_tag="VFE39_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HET6259220.1"
                     /translation="MPEGDTVYLTGKRLRAALSGRSLLRGELRHPRLVEHDLAGRTVL
                     DVATVGKHLFTRFDDGRSLHSHLRLDGSWHLYRPGMAWQRPAHQARAVLETADRTAVG
                     FALHDLELLPTDEEDRLVGHLGPDLLDPTWDSGHAAEALRRFTARGSSELGLVLLEQR
                     VMAGVGNLYKTEVCFLLGLSPWTLVRDVPDPAAAITLSRELLLRNADRPQQSTTGELA
                     RGRQTWVFERGGQRCRRCGTRVRTAEQGGADARSAGAPRGGVYARLAYWCPRCQPGPA
                     PRPKPRHH"
     gene            1026..1934
                     /locus_tag="VFE39_11265"
     CDS             1026..1934
                     /locus_tag="VFE39_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6259221.1"
                     /translation="MPVIEVDQLHKRYRDQVAVHDVSFSVEEGEIFGVLGRNGAGKTT
                     TVECIEGLRSPDGGSLSVLGLHPERDRVELRSVLGIQLQASELPDKLRVAEAVELFAS
                     FYPSPADPGELLERWGLGAKRKTAFGNLSGGQKQRLSIALALVGNPRVAILDELTTGL
                     DPQARRDAWEAIEQIRRGGVTVVLVTHFMEEAERLCDRIAVIDAGRVVALDTPAGLVA
                     GLSGGQRLRFRPSIPFEDAVLTQLAEVESVRRNGPQVEVTGGGDLLHVVTSALARHQV
                     VAVDLRLEQATLDDAFVALTGRAAEN"
     gene            1947..2738
                     /locus_tag="VFE39_11270"
     CDS             1947..2738
                     /locus_tag="VFE39_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013221994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6259222.1"
                     /translation="MNESTSTTPTLTRAPQRRAPRPLRALAAAETRLFLRDPLTVLLA
                     VALPSVILAGLGAVPALREPADVFGGLRFVDYFAPSLLAVNIAVLGLQNLPTGLATYR
                     EKGVLRRLSATPMDPTAVLVVQLVIHLVTAAAGMVLMLAVAVYVFDVPAPRHPVWFVL
                     TFLLGTSAVFAIGLVIAAVAPRARLAAGIGTVAFMLTQFFAGVYLPKFLLPEVVVRIG
                     EFVPPGIGAFQGAWTGDGPAPLQLMVMAVIAVAATAVAARFFRWE"
     gene            2743..>3470
                     /locus_tag="VFE39_11275"
     CDS             2743..>3470
                     /locus_tag="VFE39_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259223.1"
                     /translation="MSGAERGAAVAEGCGPAVGPLPGSDELLDARERRFDSLFERYAP
                     YLGLVIGTALTPFVIPSPEGFWLVTGPLVLAVGTWSYVFTSRQPARASDPRLGPVYIS
                     GLLVLSAVLVYLSPFYGFQALAGYTHSFLYLRGRWRWAGVVATAGLVAYSQLGGGLIA
                     ELNPGVIAGWVAITLINGAVAGGFTYFGTLSAQQSHRRRDIIVELNESNRRLSESLEE
                     NAALHVQLLAQAREAGVLDERARLA"
BASE COUNT          433 a         1210 c         1301 g          526 t
ORIGIN      
        1 gcgggcgact cgggggcggg aaacgggcga ctcgcggggg tgcggtcaat ggtggcgggg
       61 cttggggcgg ggggccgggc cgggttggca gcgggggcac cagtaggcga gccgggcgta
      121 gacgccgccc cgcggagctc cggcggagcg cgcatcggca ccgccctgct ccgctgtgcg
      181 gacgcgggtg ccgcagcggc gacaccgctg cccaccgcgc tcgaacaccc aggtctgacg
      241 gccgcgcgcc agctccccgg tggtggactg ctggggacgg tcggcgttgc gcagcaggag
      301 ctcgcgggag agcgtgatcg ctgccgccgg gtccgggacg tcccgcacga gggtccacgg
      361 cgacagtccg agcaggaagc acacctccgt cttgtagagg ttcccgacgc ccgccatcac
      421 ccgctgctcc agcagcacga gcccgagctc cgacgaaccg cgtgccgtga accggcgcag
      481 cgcctccgcg gcgtgcccgc tgtcccatgt ggggtcgagc aggtcaggcc cgaggtgtcc
      541 gaccagccgg tcctcctcat cggtcggcag tagctccagg tcgtgcaagg cgaacccgac
      601 ggcggtgcga tcagcggtct cgagcaccgc cctggcctga tgggcgggcc gctgccacgc
      661 catccccggg cggtagaggt gccacgagcc gtccagtcgg aggtggctgt gcaggctgcg
      721 gccgtcgtcg aagcgggtga acaggtgctt gccgacggtc gcgacgtcga gcacggtgcg
      781 tccggcgagg tcgtgctcca cgagccgcgg atggcgcagc tcgccccgca ggagcgaccg
      841 gccggagagc gcggcgcgta ggcgcttccc ggtgaggtag accgtgtcgc cctccggcac
      901 ggggccgacg gtagcgcggg ccgggctggt ccaccgatcg gttgagccgg ggtccacctc
      961 cggccacaga gactccgccg cgacggtcga tctccggggg cgcaggcgga agcacggttg
     1021 ttcccatgcc cgtcatcgag gtggatcagc tgcacaagcg ctaccgcgac caggtcgcgg
     1081 tgcacgacgt ctccttctcc gtggaagagg gggagatctt cggcgtcctc ggccgcaacg
     1141 gcgctgggaa gaccaccacc gtggagtgca tcgaggggtt gcgctccccg gacggaggct
     1201 ccctctcggt gctcgggctg caccccgagc gggaccgcgt ggagctgcgc agtgtgctcg
     1261 gcatccagct gcaggcgagc gagctgcctg acaagttgcg tgtcgcggag gccgtcgagc
     1321 tgttcgcgtc gttctacccg tcgcccgccg acccgggcga gctgctcgaa cgctggggtc
     1381 tcggcgcgaa gcggaagacc gcgttcggga acctctcggg gggccagaag cagcggctct
     1441 cgatcgcgct ggcgctggtg gggaacccac gggtggcgat cctcgacgag ctgaccacgg
     1501 ggctcgaccc gcaggctcgc cgcgacgcct gggaggcgat cgagcagatc cgccgcggtg
     1561 gcgtgacggt cgtgctggtc acccatttca tggaggaggc cgagcggctc tgtgaccgca
     1621 tcgccgtgat cgacgcggga cgcgtggtcg cgctcgacac cccggcaggg ctcgtggccg
     1681 ggctcagtgg cgggcagcgg ctgcgcttcc gcccgtcgat cccgttcgag gacgcggtgc
     1741 tcacgcagct tgccgaggtg gagtcggtgc ggcgcaacgg cccgcaggtc gaggtcacgg
     1801 ggggcggcga cctgctgcac gtcgtcacgt ccgcgctggc ccgccaccag gtcgtcgccg
     1861 tcgacctgcg cctcgagcag gccaccctcg acgacgcgtt cgtcgccctg accggccgtg
     1921 cggccgagaa ctgaggagcc cgaccgatga acgagagcac gtccacaacc ccgaccctga
     1981 cccgggcgcc gcaacgccgc gcgccgcggc cactgcgcgc actcgcggcg gcggagaccc
     2041 ggctgttcct ccgcgatccc ctcacggtgc tgttggcggt cgccctgcca tcggtgatcc
     2101 tcgcgggtct cggcgccgtc ccggcgctcc gcgagcccgc cgacgtcttc gggggtctcc
     2161 ggttcgtcga ctacttcgca ccgtccctac tggcggtcaa catcgccgta ctcggcttgc
     2221 agaacctgcc caccggactc gccacctatc gggagaaggg cgtgctgcga cggctgtcgg
     2281 ccacgccgat ggacccgaca gcggtactgg tggtgcagtt ggtgatccac ctcgtgacgg
     2341 ctgccgccgg catggtgctg atgctcgccg tcgccgtgta cgtcttcgac gtgccggcgc
     2401 ctcgccaccc ggtctggttc gtcctcacgt tcctgctggg cacctcggca gtgttcgcga
     2461 tcgggctcgt gatcgcggcc gtggcgccgc gggcccggct cgccgccgga atcggcaccg
     2521 tcgcgttcat gctgacgcag ttcttcgccg gggtgtatct gcccaagttc ctgctcccgg
     2581 aggtggtcgt gcgcatcggg gagttcgtcc caccgggcat cggggcgttc cagggcgcct
     2641 ggacgggaga cggaccggcc ccactgcagc tgatggtgat ggccgtgatc gcggtggcgg
     2701 cgacggccgt tgccgctagg ttcttccggt gggagtgagg ccgtgagcgg cgccgagcgg
     2761 ggtgcggcgg tggcggaggg ctgcggcccc gcggtgggcc cgctgccggg cagcgacgag
     2821 ctgctcgatg cccgcgagcg caggttcgac tcgctcttcg aacgctacgc gccctatctc
     2881 ggactggtca tcggtacggc gctcacgccg ttcgtgatcc cgtctccaga ggggttctgg
     2941 ctggtcaccg gcccgctcgt gctcgcagtc ggaacctggt cctacgtctt cacgagccgg
     3001 caaccggcgc gcgcgtcgga cccccggctg ggcccggtgt acatcagcgg gctgctggtc
     3061 ctgagcgcgg tgctcgtgta tctgtcgccc ttctacgggt tccaggcctt ggccgggtac
     3121 acgcactcgt tcctgtacct gcgggggcgc tggcgctggg cgggcgtcgt ggccacggcg
     3181 gggctcgtcg cgtactccca gctcggcggc ggactgatcg ccgagctcaa cccgggcgtc
     3241 atcgcgggct gggtggcgat cacgctgatc aacggcgcgg tggcgggggg cttcacctac
     3301 ttcggcacgc tgtccgccca gcagtcccac cgcaggcggg acatcatcgt cgagctgaac
     3361 gagtccaacc ggcggctctc ggagtcgctg gaggagaacg cggccctgca cgtccagctg
     3421 ctggcccagg cgcgggaggc cggcgtgctc gacgagcggg cccgcctggc
//
LOCUS       DASRSC010000229         4289 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_905694,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000229 DASRSC010000000
VERSION     DASRSC010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4289
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905694"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFE39_11280"
     CDS             <1..604
                     /locus_tag="VFE39_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017985454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259224.1"
                     /translation="RLAVRLRAAGHTVVALDEGDRDRERWAAATREAALSGTRAKALA
                     AAAVRADQVERVIRPALESGAVVVVDRFLVSPLVQFGVAADRAHDELDPGELENLAAW
                     ATGRLRPDVSVLLDRAPTGPVSPVRGISGEEHVRVQRLLTRMAAAEPHRYVVVDADGS
                     ADDVAERVYTGLQPLLPARPTAPADGAVPDSPAAATAPAP"
     gene            601..1752
                     /locus_tag="VFE39_11285"
     CDS             601..1752
                     /locus_tag="VFE39_11285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET6259225.1"
                     /translation="MTVWDEVVGQPSAVAELSAAAENPAAMTHAWLFTGPPGSGRSVA
                     ARAFAAALQCPFHGCGQCPSCHTVMTGTHSDVREVVPEGLSISVRSMRQLVHSAARRP
                     VSGRYQVLIITDADRLTENAANALLKFIEEPPDQTIFLLCAPSDHPEDVQITIRSRCR
                     PVPLRTPSPGAIADVLVRRDGIEPETARWAASVAGGHVGRARRLARDADARTYRETVL
                     GLPMRVRGLGDAFAYAGELVRNAKAEATELSETRDAIEREELAVAMGAGGVGKGVAAA
                     ARGAKAAEKDLERQQKSRNTRNQRDAIDRALVDLAGFFRDALVVASGARVALTNPDRD
                     ADIRRAAAEWSPESVLRRLEAVLTCRTALDQNVKPEIAVESMMTALQRG"
     gene            complement(1789..2517)
                     /locus_tag="VFE39_11290"
     CDS             complement(1789..2517)
                     /locus_tag="VFE39_11290"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6259226.1"
                     /translation="MEDRPLQLIDLGYVCELYREFAGELAAVGKAQREYAESMKATMT
                     PQLDDLEAEITYLLLRATEPAAVMELGTFHGWSTSWILSALRDNGSGHLHSFDMIDNV
                     VRNVPHDLAEGRWTFVQGDIRNTLDQVPTDTGYLFVDAAHNSRFARWYLHNLFPRVPS
                     GIPVSVHDAFHFRTTLPLHEGKVVVRWLKEKDVPFFTPSRAKARADYDALNKLRNELG
                     LAPIRGAGDNPMIYFRMPSLSATA"
     gene            2649..2725
                     /locus_tag="VFE39_11295"
     tRNA            2649..2725
                     /locus_tag="VFE39_11295"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2683..2685,aa:Thr,seq:cgt)
     gene            2834..3133
                     /locus_tag="VFE39_11300"
     CDS             2834..3133
                     /locus_tag="VFE39_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259227.1"
                     /translation="MARGSIETLRTGFRARVYVGKDPITGKQTYHRGETRRNRRDAEA
                     DRRPDLHTTVGTLLDRWMEIVDHELSTAETTAGYVRRTIKPALGDMPLRKLQRGG"
     gene            complement(3572..3844)
                     /locus_tag="VFE39_11305"
     CDS             complement(3572..3844)
                     /locus_tag="VFE39_11305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259228.1"
                     /translation="MLTGADNEHPWRATIPSSRFGQRPSEPVEYQRVTPKTVVELDVD
                     ASFDDHRWRHAARFVRLPASTIRPSEVLGSGSDASARRIRSGSTRP"
     gene            complement(3873..>4289)
                     /locus_tag="VFE39_11310"
     CDS             complement(3873..>4289)
                     /locus_tag="VFE39_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259229.1"
                     /translation="RVGLLGDAAFSSSPMSGGGTGLALVGAYLLAGELAAADWDPEAG
                     FAGYEERMRSFVEANQEIGRLHVHSRDVPAPDAEPSPEPDMEALMELVERAINGVELP
                     DYAGVPDSGARHSPRHSSSADTISVAGCMSSAAPGR"
BASE COUNT          622 a         1486 c         1559 g          622 t
ORIGIN      
        1 gcggctggcc gtgcgcctgc gggccgcggg tcacacggtg gtcgccctgg acgagggcga
       61 ccgcgatcgc gagcggtggg ccgctgccac ccgcgaggcg gcgctgtcgg gcacgcgggc
      121 gaaggcgctc gccgcggcgg cggtgcgggc cgaccaggtc gagcgggtga tacggccggc
      181 gctggagagc ggtgcggtcg tggtcgtcga ccggttcctg gtgagcccgc tggtgcagtt
      241 cggggtggcg gccgaccgcg cgcacgacga gctcgacccc ggcgagctgg agaacctggc
      301 agcctgggcc accggccgac ttcgtcccga cgtgtccgtg ctgctcgacc gcgccccgac
      361 cggacccgtg tcccccgtgc gcggcatctc cggcgaggaa cacgtgcgag tgcagcggct
      421 gctcacccgc atggcggccg cggagccgca ccgctacgtc gtcgtcgacg ccgacggctc
      481 ggccgacgac gtggccgagc gcgtctatac cggtctacag ccgctcctgc ctgcgcggcc
      541 caccgccccg gccgatggcg cggtccccga ctcgccggcg gcggctacgg ccccggcgcc
      601 gtgaccgtct gggacgaggt cgtcgggcag ccgtcggccg ttgccgagct gagcgcagcc
      661 gccgagaacc ctgcggccat gacgcacgcc tggctgttca cgggcccgcc cggctccgga
      721 cggtccgtgg cggcgcgcgc cttcgcggcg gcgctgcaat gcccgttcca cggttgcggg
      781 cagtgcccgt cctgccacac ggtgatgacg ggcacccact ccgacgtccg cgaggtcgta
      841 cctgaagggc tgtcgatctc ggtgcgatcg atgaggcagc tcgtgcattc ggcagcccga
      901 cgtccggtca gcggccgcta ccaggttctg atcatcaccg acgccgaccg gctcaccgag
      961 aacgcggcca acgcgctgct caagttcatc gaggagccgc ctgatcagac gatcttcctg
     1021 ctgtgcgcgc cgtccgacca tcccgaggac gtccagatca ccatccggtc acgatgccgt
     1081 ccggtgccgc tgcgcacccc gtcaccgggg gccatcgccg acgtgctggt ccgccgggac
     1141 gggatcgagc cggagacggc gcgctgggcg gcatcggtcg cgggtggcca cgtcggccgg
     1201 gcgcgcaggc tcgcgcgtga tgccgacgcg cggacgtacc gcgagacggt gctggggctg
     1261 ccgatgcgtg tccgggggct cggagatgcg ttcgcctacg cgggcgagct ggtccgcaac
     1321 gcgaaggcgg aggccaccga gctcagcgag acccgcgacg ccatcgagcg tgaggagctg
     1381 gcggtcgcca tgggcgccgg tggcgtgggc aagggggtcg ccgcggccgc gcggggggcg
     1441 aaggcggcgg agaaggacct ggagcgccag cagaagtcgc gcaacacccg caaccagcgc
     1501 gacgcgatcg accgcgccct ggtcgacctg gccggtttct tccgcgacgc gctggtggtg
     1561 gccagtggcg cacgggtggc gctgacgaac cccgaccgcg acgccgacat ccgacgggcg
     1621 gccgccgaat ggtcgcccga gtcggtgctg cgcaggctgg aggcagtgct cacctgccgc
     1681 acggcgctcg accagaacgt caagccggag atcgccgtcg agtcgatgat gacggcgctg
     1741 caacgggggt gatccacgag gcgcgcgacc cgcgacgcct cgccggtctt atgcagtcgc
     1801 cgacaacgac ggcatcctga agtagatcat cgggttgtcg cctgcgcccc gtatgggcgc
     1861 gagcccgagc tcgttgcgca gcttgttcag cgcgtcgtag tccgcacgcg ccttcgcccg
     1921 agacggggtg aagaagggca cgtctttctc cttcagccag cggacgacca ccttgccctc
     1981 gtgcaggggg agcgtggtgc ggaagtggaa ggcgtcgtgc acactcaccg gaatcccgga
     2041 cggcacccgc gggaacaggt tgtggaggta ccaccgggcg aagcggctgt tgtgcgcggc
     2101 gtcgacgaac aggtagccgg tgtcggtggg cacctggtcg agcgtgttcc tgatgtcgcc
     2161 ctgcacgaac gtccaccgac cctctgcgag gtcatggggc acgttgcgca ccacgttgtc
     2221 gatcatgtcg aagctgtgca ggtgcccgga cccgttgtcc cggagagcgc tgaggatcca
     2281 ggacgtcgac cacccatgga acgtcccgag ctccatcacc gcggccggtt cggtggcccg
     2341 cagcaacagg taggtgatct ccgcttcgag gtcgtcgagc tggggggtca tcgtggcctt
     2401 catcgactcg gcgtactccc gctgggcctt gcccacggcg gccagctcgc ccgcgaactc
     2461 gcggtacagc tcgcagacat accccaggtc gatcagctgc aacggacggt cctccatgtg
     2521 gatgtctcct gaagccgggc gacggccact gtatccgccg gtccggggcc taccggacgg
     2581 ggtgacggcc actggacggt gggcgtcgag ggtgcggagg cggtcgctac actgaccggg
     2641 caccatcggc cgccttagct cagtcggtta gagcgacgca ctcgtaatgc gtaggtctgg
     2701 ggttcgactc cccaaggcgg ctccacagaa gtagcaggtc acagcgttgc tacttaccat
     2761 cgatgatcga atcagtgccc taggttggca tgattcttgg catgtcggcc tttgtcgggc
     2821 ctacctttag cccgtggcga ggggttcgat cgagacgttg cgcaccggct tccgtgcgcg
     2881 cgtgtacgtc ggcaaggacc cgatcacggg gaagcagacc taccatcggg gcgagacccg
     2941 ccgaaaccgc cgggacgcgg aggccgaccg gcggcccgac ctccacacca cggtcggcac
     3001 gctgttggac cgctggatgg agatcgtcga tcacgagctg agcaccgccg agacgacggc
     3061 cggctacgtg cggcggacga tcaagccggc gctcggggac atgccactgc ggaagctgca
     3121 gcggggcggg tgatcgacga tccgctcgag actgggacgg ggtcaacatc ttcgccgagc
     3181 cacagctgga ggacatgtac cccgatggcg acggggcggc cgccggcacc gccgggtgag
     3241 cggccgttcg cgtattccgc gtggtcggcg ctacccgggc aggaggccgc gggcaggctc
     3301 gtcgtgacac gagccatgac acggaaaccg cgttggttgc ggctctcctg gataggcgcc
     3361 gagcacgggc ggcggttcac cgagcgcgcc tacaacgcct tacaccgagc ggtcgcaagg
     3421 tcgaaccctg tctgccgcgg gcaccagcac tatgcggcca gcggctctcc gggcttgggc
     3481 ccgcaacggg cggtgcgttc ggcatgtctg cgccagcagc gctgcaagga gggcaacgcc
     3541 atccggctga tcctccaacc ctgatcgacg ttcacggccg ggtggaaccg gagcggatcc
     3601 gccgcgcact ggcatcactg ccgctgccca ggacctcgga cgggcggatc gtgctggcgg
     3661 gcaggcggac gaaacgcgcc gcgtgccgcc accggtgatc gtcgaatgag gcgtcgacgt
     3721 cgagctccac aacggttttc ggcgtgaccc gttggtactc caccggctcg ctcggtcgtt
     3781 ggccgaaccg actcgacggg atcgtcgccc gccacgggtg ttcattgtcg gcaccggtca
     3841 gcacgtggcg atgtcgttgt gtgccgaccc ggtcagcggc cgggtgcggc cgacgacatg
     3901 cagccggcca cgctgatcgt gtcggccgag gacgagtgcc tcgggctgtg ccgggccccg
     3961 gagtccggga ccccggcgta gtcgggcagc tcgacgccgt tgatcgcgcg ctccaccagc
     4021 tccatgagcg cctccatgtc gggctccggg ctcggctcgg cgtccggcgc ggggacatcg
     4081 cggctgtgca cgtgcagccg gccgatctcc tggttggcct cgacgaacga gcgcatccgc
     4141 tcctcgtagc ccgcgaaccc ggcctccggg tcccagtcgg ctgcggccag ctccccggcc
     4201 agcaggtacg cgccgaccag tgccagcccg gtgccccctc cggacatcgg cgacgagctg
     4261 aacgccgcgt cgccgagcag ccccacgcg
//
LOCUS       DASRSC010000230         5811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_577876,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000230 DASRSC010000000
VERSION     DASRSC010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577876"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(621..1898)
                     /gene="tyrS"
                     /locus_tag="VFE39_11315"
     CDS             complement(621..1898)
                     /gene="tyrS"
                     /locus_tag="VFE39_11315"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020629629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET6259230.1"
                     /translation="MGIDILDELEWRGLIAQSTDLDALRRDLAEGVLTLYAGFDPTAP
                     SLHAGNLVPLLTLRRFQQAGARPIVLAGGATGMIGDPREVGERSLNSAETVTGWAERI
                     RGQLERFVEFDDSPTGALIANNLEWTGPQSVLEFLRDVGKHFSINVMLGRETVKRRLA
                     TDGMSYTEFSYLLLQSQDYLHLHRRYGCALQVGGSDQWGNILGGVELVRRVEGAAVHA
                     LTTPLVTDSEGRKFGKSTGGGSLWLDPELTSPYAWYQYFLNVSDADVGRYLRLFTFLP
                     PEEIEELEKSVAERPHLRAGQRRLAEELTTLVHGRRHTEQVIAASAALFGRGDLHDLD
                     AGTLEAALSEVPHATVRLDERPTVVDLLVATGLAESRGAARRTVGEGGAYVNNAKVPD
                     EQWTPEEHDLLPGRWLVVRRGKRHLAGARAVDG"
     gene            complement(1933..2136)
                     /locus_tag="VFE39_11320"
     CDS             complement(1933..2136)
                     /locus_tag="VFE39_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259231.1"
                     /translation="MEWITIPGTANSGLDQQSGHPALRVGREGRVVDLTEEQGVPLSF
                     SSVLSLPALLDELPAEFDAHLGT"
     gene            complement(2235..2867)
                     /locus_tag="VFE39_11325"
     CDS             complement(2235..2867)
                     /locus_tag="VFE39_11325"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005163246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HET6259232.1"
                     /translation="MTLLDREELATDVLLAAERLLGCTVEADTPEGTVAVRLVEVEAY
                     RGADDPASHSFRGRTARNGVMFGPAGHLYVYFIYGMYFCANISCLREGEAGAVLLRAG
                     EVVSDLAVAHVRRSTARRSADLARGPARLATLLGLGREDNGVDLTDPGSPVRLLAASP
                     VDPALVRSGPRVGVAAAHELPWRFWLDGSPAVSPYRPGRGASAGQLGPER"
     gene            complement(2864..4159)
                     /locus_tag="VFE39_11330"
     CDS             complement(2864..4159)
                     /locus_tag="VFE39_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6259233.1"
                     /translation="MTSPADLAHPALDLDAARFAYDELRAQGLALDLTRGKPSSAQLD
                     LAGPMLSLPGERYRTADGIDTRNYQGLQGLPELRAVFSPILDVPVEQLIAFGNSSLEL
                     MHDTVVHALLSPLPGAERRWVDEDRIAFLAPTPGYDRHFALCERLGVELVQVPMTPDG
                     PDMDVVEELVGADPSVKGIWCVPKYSNPDGAVYSEDTVRRLASMPAAAPDFRIFWDNA
                     YAVHHLTDDEVEIPDVLTLAAEAGHEDRPFVFGSTSKITLAGAGVAFLGASPANVSWW
                     LALTAKRTIGPDKINHLRHAMFLRDADGVREHMRRHRELIAPKFAAVDRVLTERLGSV
                     PGVRWSRPAGGYFVTLTVPDGTAAEVVRLAGEAGIALTPAGATHPYSTDPEDRTIRLA
                     PTFPELAEVERAMSGVTDCVRLALASRRAEREHGDTTAQ"
     gene            complement(4156..5583)
                     /gene="argH"
                     /locus_tag="VFE39_11335"
     CDS             complement(4156..5583)
                     /gene="argH"
                     /locus_tag="VFE39_11335"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET6259234.1"
                     /translation="MSSNLWGGRFASGPADALAALSKSTHFDWRLAPYDLRGSKAHAR
                     VLARAGLLTDDELAGMLDALAKLEADVESGAFGPAPDDEDVHSALERGLIDRAGPELG
                     GKLRAGRSRNDQVATLFRMWLRDAARRVGARVVDVVDALVAQAGAHPDAPMPGRTHLQ
                     HAQPVLLAHHLAAHAHALLRDVDRLRDWDRRAAVSPYGSGALAGSSLGLDPQAVAAEL
                     GFDSSSANSIDGTAARDFAAEAAFVLAMIAVDLSRIAEEVIIWATAEFSYVTLDDAFS
                     TGSSIMPQKKNPDVAELARGKAGRLIGNLAGLLATLKAMPLAYNRDLQEDKEPLFDSV
                     EQLELLLPAVAGMVATLTFHTDRLAELAPTGFTLATDVAEWLVRAGVPFRTAHEAAGG
                     CVRAAESRGVGLADLTDAELVAVHPSLTPAVREVLTVAGSIASRNARGGTAGERVAEQ
                     LDALRAAAAAARGALAPAALTGGPA"
     gene            complement(5580..>5811)
                     /locus_tag="VFE39_11340"
     CDS             complement(5580..>5811)
                     /locus_tag="VFE39_11340"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007025565.1"
                     /note="catalyzes the formation of
                     2-N(omega)-(L-arginino)succinate from L-citrulline and
                     L-aspartate in arginine biosynthesis, AMP-forming; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HET6259235.1"
                     /translation="FVAQTQEYVTGEVRMTLHAGRAVPTGRRSDASLYDFNLATYDEG
                     DVFDQSLAKGFVQLWGLPSKIAAKRDQRVRGS"
BASE COUNT          856 a         2066 c         2049 g          840 t
ORIGIN      
        1 ctagcaaacc tgcgacgggg tcgcaggtaa aaacaaactg gcataaaacc aattcatgat
       61 aaagctcgac acactgttga gttctcaaaa gacacccgca caccatccac caccgctagg
      121 cagtgctccg gggctgtgtt ctcgtctgcg tcgccaccgg acagtgccgg agcaactttt
      181 ccacgataca tggtcctgac cgtggagtca aaccccacca ggcccacatc cgtgggagcg
      241 caccttgcga tgcgatccct ccgggggctg cgctgcaacc ggacagtgcc cggggcaact
      301 ctccaagctt acgtggtcct tgccgtggtg tcaaaccccg ccaggcccac ttcgccagag
      361 caactctcga actgtacgtg gtcctgagcg cggggtcaag ccccaccagg cccacatcca
      421 gaaggcgctg caccttgcgg tgcggtcctt ccggggcggc cgcctgtccg gggctgactc
      481 tgggccggag gatctccgtc ccggtctgtc gtgctgcccg ccgtggcgac gaggagaaag
      541 ttacacaggc cgttcgtcgg tagtcaaatc gggggtccct cttccgcgtc tccgcaggtc
      601 agaagccgat ccgaccggct ttagccatcg acggcacgcg caccagccaa gtgccgcttg
      661 ccccgtctca ccacgagcca gcgcccgggc agcaggtcat gttcctcagg cgtccactgc
      721 tcgtcgggca ctttcgcgtt gttcacgtac gccccgccct caccgacggt gcgtcgagcg
      781 gcgccccgac tctctgcgag gccggtcgcg acgagcaggt cgacgacggt cggccgctcg
      841 tcgagccgaa ctgtggcgtg cggaacctcg gacagcgctg cctccagcgt ccccgcgtcg
      901 agatcgtgca ggtcgcctcg gccgaacaga gccgcgctcg ccgcgatgac ctgttcggtg
      961 tggcgccggc cgtgcacgag ggtggttagc tcctcggcca gcctgcgctg tccggcgcgc
     1021 agatgcggcc gttcggcgac ggacttctcc agctcctcga tctcctctgg cggaaggaac
     1081 gtgaacaacc gcaggtaccg gccgacgtcg gcgtccgaca cgttgaggaa gtactggtac
     1141 caggcgtacg gcgacgtcag ctccgggtcg agccagaggc tcccgccccc ggtcgacttt
     1201 ccgaacttgc gtccctcact gtccgtgacc agcggggtgg tgagtgcgtg gaccgccgcg
     1261 ccctcgactc gccgcacgag ctcgacgcca ccgaggatgt tgccccactg gtcggagccg
     1321 ccgacctgca gggcacatcc gtaacgccgg tgcaagtgca ggtagtcctg gctctgcagc
     1381 agcaggtagc tgaactcggt gtacgacatg ccatcggtgg ccagcctgcg cttgaccgtc
     1441 tcccgaccga gcatcacgtt gatcgagaag tgcttgccga cgtcccgaag gaactcgagc
     1501 acggactgcg gccctgtcca ctccagattg ttcgcgatca gcgcgccggt gggtgagtcg
     1561 tcgaactcga cgaaccgctc gagctgccca cggatgcgtt ccgcccagcc ggtgaccgtc
     1621 tcggcgctgt tcaacgaccg ctcgccgact tcacgcggat ccccgatcat gccggtggct
     1681 cccccggcga gcacgatggg ccgcgctccc gcctgctgga accgtcgcag cgtcagcagc
     1741 ggtacgaggt tgccggcgtg caggctcggg gcagtagggt cgaagcccgc atagagggtg
     1801 agcactccct cggcgaggtc gcgccgaagc gcgtccaagt cggtgctctg cgcgatcagg
     1861 ccgcgccact ccagctcgtc gaggatgtcg atgcccacgg caccgatcct cccccacgcc
     1921 tgccagccgt tttcaggtgc ccaggtgcgc gtcgaactcc gcgggcagct cgtcgagcag
     1981 cgccggcagg ctcagcacgc tgctgaacga caacggcacg ccctgctcct cggtgaggtc
     2041 caccacgcgg ccctcccgcc cgacccgcag cgccgggtgt ccggactgct ggtcgaggcc
     2101 ggagttcgcc gtgccgggga tggtgatcca ttccacgagg tcattctggt cgagcagccc
     2161 gagttgctcg gtgctgatct ccgcgtagga acctctgccc cgccagctgg tcgaaccagg
     2221 cgggcacggt gaaactacct ttcgggtccg agctgccccg ccgatgcgcc ccgaccgggg
     2281 cggtaggggc tcacagccgg cgacccgtcc agccagaacc gccacgggag ttcgtgcgca
     2341 gcggccaccc cgacgcgggg cccggagcga acgagcgccg gatcgacggg cgaggcggcc
     2401 aacagccgca caggcgaccc gggatcggtg aggtccaccc cgttgtcctc ccgtccgagc
     2461 cccagcagtg tcgcgagccg ggcggggccg cgcgccagat cggctgagcg gcgggcggtg
     2521 gagcggcgga catgagccac cgccaggtcc gacaccacct cgcccgcgcg cagcagcacc
     2581 gcgcccgcct ccccctcgcg aaggcagctg atgttcgcgc agaagtacat cccgtagatg
     2641 aaatagacgt acaggtgccc ggcgggcccg aacatcacgc cgttgcgtgc ggtgcggccc
     2701 cggaagctgt gtgaggcggg gtcgtcggca ccgcggtagg cctccacctc gacgagccgc
     2761 acggcgaccg tgccctccgg ggtgtcggcc tcgaccgtgc acccgagcaa tcgctcggcc
     2821 gcgaggagca cgtcggtcgc gagctcttcc cggtccagca gcgtcactgg gccgtcgtgt
     2881 ctccgtgctc gcgctcggct cggcgggagg ccagggccag gcggacgcag tcggtcacgc
     2941 cggacatggc gcgctccacc tcggccagct ccgggaacgt cggggcgaga cggatcgtgc
     3001 ggtcctcggg atcggtgctg tacggatgcg tcgcacctgc cggggtgagc gcgatccccg
     3061 cctcgcccgc cagccggacg acctcggcgg ccgtgccgtc cggcaccgtc agcgtcacga
     3121 agtagccgcc cgcaggtcgc gaccagcgta cgcccggcac cgacccgagg cgctcggtca
     3181 ggacgcggtc gacggcggcg aacttcgggg cgatcagctc ccggtgccgc cgcatgtgct
     3241 cgcgcacgcc gtcggcgtcg cgcaggaaca tcgcgtgccg caggtggttg atcttgtccg
     3301 gcccgatcgt gcgcttcgcg gtgagcgcga gccaccacga cacgttggcc ggggacgcac
     3361 ccaggaacgc gacgcccgca ccggcgagcg tgatcttcga cgtggagccg aacacgaacg
     3421 gccggtcctc gtggcccgcc tcggccgcca gcgtcagcac gtcggggatc tccacctcgt
     3481 cgtcggtgag gtggtgcact gcgtaggcgt tgtcccagaa gatccggaag tcgggggctg
     3541 cagcgggcat cgacgccagc ctgcgcacgg tgtcctcgga gtagacggcg ccgtcggggt
     3601 tggagtactt cggcacgcac cagatgccct tgaccgaggg gtcggcgccc acgagctcct
     3661 cgacgacgtc catgtcgggg ccgtcgggcg tcatcggcac ctgcaccagc tcgacaccga
     3721 ggcgctcgca cagcgcgaag tgccggtcgt agccgggggt cggggccagg aacgcgatgc
     3781 ggtcctcgtc gacccagcgg cgttcggctc ccgggagtgg gctgaggagg gcgtgcacga
     3841 cggtgtcgtg catgagctcc aggctcgagt tgccgaacgc gatcagctgc tcgaccggga
     3901 cgtccaggat cggcgagaac acagcacgca gctccgggag cccctgcagg ccctggtagt
     3961 tgcgcgtgtc gatgccgtcg gcagtgcggt atcgctcgcc cggcaggctg agcatgggtc
     4021 cggcgagatc gagctgcgca ctcgacggct tgccgcgggt gagatcgagc gcgagaccct
     4081 gggcacgcaa ctcgtcgtag gcgaagcggg ccgcgtcgag atcgagcgct gggtgtgcca
     4141 ggtcggcagg cgaggtcatg cgggtcctcc ggtcagggcg gcgggcgcga gggcgcccct
     4201 ggcggccgcc gcggcggcgc gcagcgcgtc gagctgctcg gcgacccgct cccccgctgt
     4261 accgccgcgg gcgttccgag aggctatcga gcccgccacg gtgagcacct cgcggacggc
     4321 gggcgtcagc gaggggtgca cggcgaccag ctccgcgtcg gtcaggtcgg cgagcccgac
     4381 gccgcgcgac tcggcggcac ggacgcagcc accggccgct tcgtgcgccg tgcggaacgg
     4441 gacgcccgcg cgcaccagcc actcggcgac gtcggtcgcg agggtgaacc cggtgggggc
     4501 cagctcggcg agccggtcgg tgtggaacgt cagggtggcg accatcccgg cgaccgcggg
     4561 gagcaacagc tccagctgct caacggagtc gaacaggggc tccttgtcct cctgcaggtc
     4621 tcggttgtag gccagcggca tcgccttgag ggtggcgagc agcccggcca ggttgccgat
     4681 cagccgcccc gctttgccgc gcgcgagctc cgccacgtcc gggttcttct tctgcggcat
     4741 gatcgagctg ccggtggaga aggcgtcgtc gagggtgacg taggagaact ccgctgtcgc
     4801 ccagatgatc acctcctcgg cgatccggga caggtccacc gcgatcatcg cgagcacgaa
     4861 cgcggcctcg gcggcgaagt cgcgggccgc cgtgccgtcg atggagttgg cgctcgagga
     4921 gtcgaagccc agctctgcgg ccacggcctg cggatcgagc ccgagcgatg accccgccag
     4981 tgcccccgac ccgtacggag atacggcggc gcgccggtcc cagtcgcgca gccggtcgac
     5041 gtcgcgcagc agcgcgtggg cgtgtgcggc gaggtggtgg gcgagcagga cgggctgcgc
     5101 gtgctgcagg tgggtacgcc cgggcatcgg ggcgtccggg tgcgccccgg cctgcgcgac
     5161 cagcgcgtcg acgacgtcca cgacgcgcgc gccgacccgg cgggcggcgt cccgcagcca
     5221 catccggaac agcgttgcca cctggtcgtt gcgcgatcgc ccggcccgga gcttgccgcc
     5281 gagctcggga ccggcccggt cgatgaggcc gcgctccagc gccgagtgga cgtcctcgtc
     5341 gtcgggcgcg ggaccgaacg cgcccgactc cacgtcggcc tccagcttgg ccagcgcgtc
     5401 gagcatgccc gcgagctcgt cgtccgtgag caggccggcg cgggcgagca cgcgggcgtg
     5461 tgccttcgaa ccccgcaggt cgtacggcgc cagccgccag tcgaagtgcg tggacttcga
     5521 cagggcggcg agcgcgtcgg ccggtccgct tgcgaaccgg ccgccccaga ggttcgagct
     5581 cacgagcccc gcacccgctg gtcccgcttg gcggcgatct tgctgggcag gccccacagc
     5641 tgcacgaagc ccttggcgag gctctggtcg aacacgtcgc cctcgtcgta ggtggcgagg
     5701 ttgaagtcgt agagcgaggc gtcggagcga cgcccggtcg gcaccgcgcg gccggcgtgc
     5761 agcgtcatcc gcacctcgcc cgtgacgtac tcctgtgtct gcgccacgaa c
//
LOCUS       DASRSC010000231         7166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_578151,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000231 DASRSC010000000
VERSION     DASRSC010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7166
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578151"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(889..965)
                     /locus_tag="VFE39_11345"
     tRNA            complement(889..965)
                     /locus_tag="VFE39_11345"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(929..931),aa:Arg,seq:tct)
     gene            1405..2112
                     /locus_tag="VFE39_11350"
     CDS             1405..2112
                     /locus_tag="VFE39_11350"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6259236.1"
                     /translation="MRPGSTTMWESNIAAWTAQRTVHAVEILGEAGLSVQGRPIRDAD
                     DQAAWLAATLRGLELGPAHVLGVSSGGWLVVNLALHDPDPVASLSLIDPACVFGRFPL
                     KIVVASLATLPAAPKFLRNRTLRWISGGVAVDDEPIARVIASAMTKFRLAVPAPTYPG
                     DDELRALRVPTLALIAGRSEIHDGRKAFERARSLLPDVRAELWPEATHAVTGQFSDEV
                     NAVVLDFLRRVDDRRSE"
     gene            2129..2800
                     /locus_tag="VFE39_11355"
     CDS             2129..2800
                     /locus_tag="VFE39_11355"
                     /inference="COORDINATES: protein motif:HMM:NF017530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HET6259237.1"
                     /translation="MVTITEDLVLLLFDPESGRAVVDSTSLDRAIGGALLLDLSTRDR
                     LTAEGADAKARLFVADSSATGDPLLDAALARLDGRNPLRAQKAVERLARKTREPVLQR
                     LVERGLVRRDRGRLLGIIPVTTWPAGDAGPVTELRGRVAAVLRDGAAPDQHLALLISL
                     LHAVKAEHKLVEGPRRQLRARAEEVAQGDWAGPAVRKAVQAVQTSVAVAVAASASAAG
                     SAGGS"
     gene            2932..3726
                     /locus_tag="VFE39_11360"
     CDS             2932..3726
                     /locus_tag="VFE39_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016337966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HET6259238.1"
                     /translation="MSPTLRSAAGSDVGLRRSMNQDSAATTPRLLVVADGMGGHAHGE
                     VASSVAVAALLDLDAQLRGTDLSGVDLPAALGGGIADAAARLTQLSEQNPELQGTGTT
                     VVAFLVHGTRIGVAHIGDSRAYLLRAGELVRLTRDHTLVQSLIDEGRISPEEAASHPR
                     RSWLIRTLQESTPAEPDLFPIDGLVGDRYLICSDGVTAVLDDAAIRDALSTVAEPADA
                     VAQLITRANEGGGPDNITCIVADLVDQAPEPGEVQVVVGAAERSEF"
     gene            complement(3773..4495)
                     /locus_tag="VFE39_11365"
     CDS             complement(3773..4495)
                     /locus_tag="VFE39_11365"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6259239.1"
                     /translation="MTDGAGTVDGCVAERLLRAATGPGGGGPVRWSAAPLVTAERVLE
                     LCCGTGVLADELPAGHWLGVERGVTPASRRPLLRATPCALPLRGGAVDAVAILLALPR
                     LPDLDTMFAEVRRVLRPGGTLVVVVPSAVSRTVGELRLAPLLSPVHRSGWRHRAALDH
                     TGWLLAAADFAVLSDDRVPFTLPLPDVAAAHDLVADLPRAGLWPPDLPAAVRTRVAAG
                     LARRAGHGRMLPIPLRRLVARR"
     gene            4581..6821
                     /locus_tag="VFE39_11370"
     CDS             4581..6821
                     /locus_tag="VFE39_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HET6259240.1"
                     /translation="MAHATVEPAAPPPQTRPPSGLAGVVGLGVAVAVMVAASLTALTD
                     ARPVTSLGLPDPGVLTNVGLPAVRAGTEALMVLTIGALLLAAFLVSPQRAGYLDVGGY
                     RALRAASWTAAGWAAGSILLIPLNVADALGRPVGDVLDPGLLIDLIPRLSAATAWSLT
                     ALVALLVLVGCRTVLTWGWSAVLFGLALLGPLPVALTGHSAGGGAHDIASDSLVLHVL
                     AASLWVGGLVALLGIATTRGPDRSSALATAVPRFSRLALACWLVLAVTGVVNALVRIP
                     LTALIGSEYGALVMAKVAALIVLGALGAAHRRSSVGPASQGDTRALLRLGGVEVLLML
                     ATIGLAVALGRSAPPDTGGVPSRTEAVIGYDLSGPPTLAGLAFDWRFDLVFGTAAIVL
                     AIAYLLGVRRLRRRGDAWATGRTLAWLVGCLALLVATSSGIGRYAPAMFSVHMGQHMI
                     LGMLVPILLVLGGPVTLALRVLPPSGRAGPPGPREWLLAAVHSPIARWLTHPLVALAL
                     FVGSYYVLYFSGLFPAALPQHWAHKLMNLHFLLVGALFFWPIVGIDPAPRRLPPPARM
                     GLVFASVPFHAFFGVAVMSANTALGGEYYQSLALPWVPDPLHDQHLGGGLAWAAGEVP
                     LLLVVIALLVQWSRQDERSARRDDRRADADGDSDLEAYNAMLRRLATNGPPLVAQSDE
                     SGYGSGHTEDVARTTPTPPHDPSDGDSSAVSTAQRHDQSTVDGLRGTRNDRPLVARRD
                     PDRDVE"
BASE COUNT          951 a         2500 c         2616 g         1099 t
ORIGIN      
        1 agcggggtgg tacgtggact cgcgcctcgt ctcgggccgc gagccgggca gcggccgggg
       61 ccgcccggca ggacatcgcc cgcaggcgaa cccatcgcag cgcaaacgcg cgggacgggc
      121 agcggcgaca tcgacttgga ctctgtcatc agccatgccc ggcgtgacgt gtccgcgagc
      181 cagcggttga cctgtcgaga gcacccgcac ccgcgcttgc gtaccaggat cgggttgggg
      241 agggcgtcgg cgggaacgcg ccgaagatcg accggacgga cgcgccccat gcgggccaga
      301 agtcgacata cgccgccgtc ctgctgcctg cgcgcgacgc atcgcggaca ttggtggaga
      361 tccgcgctgc ccagtcgttg ggctgcgcct ggagctcgag caacggtgcc gccgctgagt
      421 cgccgatccg cacgaccccg agagctgtac gccgaagaag cggacacgct ctcctccgag
      481 gttgttcgcc tcccggtgct catcgcggaa gcttggtgcc acccagacca cggcgtcggt
      541 accggcggcg taggtgatca gctggccgag gtgactgtgg tcggtctggg tcagctggtt
      601 ctcgacgatc agaacgcagt catgagtgag gtccagaccg atgaggtcca gtgaggacgc
      661 gccgacgcga tgctcggcgt tggtcagctc gaggtcgatc ccgaggacgt cggcgagcac
      721 gtccgcgcgg cgagcagcca cggcgtcatg tcgggcctcg tgcgcccaca cggtccacgc
      781 gtcgatgcgc tgcagccgtc cgagctccat gccgcgacgc taacagcgcc ctcgctccat
      841 gaataactcc acgcaccgtg ctagcaggga acgctcaagg ctgtgaactg gtgcccccgg
      901 caggactcga acctgcgacc tagagattag aaggctcttg ctctatccag ctgagctacg
      961 agggccggag cggtccagcc gaaccgccac gaagagggta gcgggacgca cgcacgagcg
     1021 ggtggcaggt tcggaacttc gatcatcgcc caacgcggcg ggttccgaac gcgcacctct
     1081 ctccgtcgtg tcccgccgga gctatctacc gccgcccagc cgatcggtcc gataccgtac
     1141 cgatcggtca cggggacgcg gtgatcactg caccggtact gacctgctgc ggcacgtcgc
     1201 cgtgcggccc cgggccgtat ccgcggacgc agcggggacc tcgacggccc cgtgatcggc
     1261 ggtcagctgc tgaggccgag agggtggcgc ggcggcgctg ctggccgtca gggcgggagg
     1321 ggtcggggga tctcatgatc gggcacttcg ctgacgccgg cgccgaggcg cggttccgga
     1381 ccgcctatgt ggagggtggc ccgcatgcgg cccggcagca cgacgatgtg ggagtcgaac
     1441 atcgcggcct ggaccgcgca gaggaccgtt cacgcggtcg agatcctcgg cgaggccggc
     1501 ctgagcgtcc agggacggcc catccgcgac gccgacgacc aggccgcctg gctggcggcg
     1561 accctgcggg ggctggagct cggcccggcg cacgtgctcg gggtgtcctc cggcggctgg
     1621 ctggtggtga acctcgcgct gcacgacccg gacccggtcg cgtcactgag tctgatcgat
     1681 ccggcgtgcg tcttcgggcg ctttccgctg aagatcgtgg tggcgtcgtt ggcgaccctg
     1741 cccgcggccc cgaagttcct gcgcaaccgc acgttgcgtt ggatctcggg cggagtggca
     1801 gtcgacgacg agccgatcgc gcgcgtgatc gcgtccgcca tgacgaagtt ccggctggcc
     1861 gtcccggcgc cgacgtatcc cggcgacgac gagttgcgcg ctctccgcgt tccgacgctt
     1921 gcgctgatcg ctgggcggag cgagatccac gacgggcgca aggcgttcga gcgggcccgg
     1981 tcgctgctgc ccgacgtgcg ggccgagctg tggcccgagg ccacacacgc ggttacgggt
     2041 caattctccg acgaggtgaa cgccgttgtg ctggacttcc tccgacgagt cgacgaccga
     2101 cggtcggagt gagcggaagg tatgtgggat ggtgaccatc accgaggacc tcgtcctcct
     2161 tctcttcgat cccgagtccg gccgggccgt cgtcgacagc acgtcgctcg accgtgccat
     2221 cgggggcgcg ttgctgctcg atctgtcgac gcgtgaccgg ctcaccgccg aaggcgcgga
     2281 cgcgaaggcg cggctcttcg tcgcagactc ctccgccacc ggcgacccct tgctcgacgc
     2341 cgcgctggcc cggttggacg gccgcaaccc gctgagggcc cagaaagccg tggaacggct
     2401 ggcgcgcaag acgcgggagc ctgtgctgca gcgcttggtc gagcgggggc tggtgcggcg
     2461 cgaccgcggc cggctgctgg gcatcatccc cgtcacgacg tggcccgccg gcgatgcggg
     2521 gccggtgacg gagctgcgcg ggcgagtggc cgcggtactg cgggacgggg cggcccccga
     2581 tcagcacctc gccctgctga tctccttgct gcacgcggtc aaggccgagc acaagctcgt
     2641 ggaggggccg cgtcggcagc tgcgagcccg cgccgaggag gttgcgcagg gcgactgggc
     2701 cggtccggcg gtacgcaagg ccgtgcaggc ggtgcagacc agtgtcgcgg tggccgtcgc
     2761 ggcctcggcc tcggcggcgg gctccgccgg tggctcctag cgagaccggg ccccatgcca
     2821 gcgaccgcag ccggtgtccg gctcagcgag gcggcggcat catccccgtc cggggcacgc
     2881 gggcgtgccg ttggacgatc agtttctgta ccgcgtgaga gggtgagcgc catgtcaccg
     2941 acgctgcgtt ccgcggcagg ctccgacgtc gggctccgaa ggtcgatgaa ccaggattcg
     3001 gccgccacca ccccgagact gctcgtcgtc gcagacggca tgggcgggca cgctcacggg
     3061 gaggtggcca gctcggtcgc cgtcgccgca ctcctcgacc tcgacgctca gctgcgcggc
     3121 acggacctct ccggggtcga cctgccggcc gcgctcggtg ggggcatcgc cgacgcggcc
     3181 gcccggctga cgcaactgag cgagcagaac ccggagctcc agggcaccgg caccactgtc
     3241 gtcgcgttcc tcgtgcacgg tacgcggatc ggtgtggcgc acatcggaga ttcgcgggcc
     3301 tatctactgc gcgcgggcga gctggtccgg ctcacccgcg accacaccct ggtccagagc
     3361 ctgatcgatg aggggcgcat ctcgccggag gaggcagcct cccacccccg ccggtcgtgg
     3421 ctgatccgga cgttgcagga gagcacgccg gccgaaccgg acctcttccc gatcgacggc
     3481 cttgtcggcg accgatattt gatctgttcc gacggggtca cggcggtact cgacgacgcc
     3541 gcgatccggg acgccctctc gaccgtggcg gagccggcgg acgcggtcgc gcaactcatc
     3601 acgcgggcca acgagggcgg cggaccggac aacatcacgt gcatcgtggc cgacctggtc
     3661 gaccaggctc cggagccagg cgaggtacag gtcgtggtgg gcgccgccga gcgcagcgag
     3721 ttctgaccgc cgcatggggt agggagcggg tcgccgcgga tccggcatgg agtcagcgcc
     3781 gcgccaccag ccgccgcagc ggtatcggga gcatgcgacc gtggccggcc cggcgggcga
     3841 gcccggccgc cacccgcgtg cgtacggcag cgggcaggtc cggcggccac aagcccgcgc
     3901 gggggaggtc ggccacgagg tcgtgcgcgg ctgcgacatc gggtagcggg agcgtgaacg
     3961 gcacgcggtc gtcgctgagc acggcgaagt cggccgccgc gagcagccag ccggtgtggt
     4021 cgagcgcggc gcggtgccgc catccggagc ggtgcacggg ggagagcagc ggggcgagcc
     4081 ggagctcccc gaccgtccgg gacaccgcgg acggcacgac caccacgagc gtgccgccgg
     4141 ggcggagcac ccggcggacc tcggcgaaca tcgtgtccag gtcgggcagg cgggggaggg
     4201 cgagcaagat cgcgacggcg tcgacggcgc cgccacgcag cgggagcgca cagggcgtgg
     4261 cgcgcaggag cggacgtctg gacgcgggcg tcacacctcg ctcgacgccc agccagtgac
     4321 cggcggggag ctcgtcggcc agcacgccgg tgccgcagca gagctcgaga acgcgctcgg
     4381 ccgtgaccag gggcgccgct gaccaccgca cgggcccgcc accgcccgga ccggtggcgg
     4441 cccgcagcag ccgctcggcg acgcagccgt ccacggtccc cgccccgtcc gtcacggggg
     4501 gagtttcgca gggctcaacc ttccgtcccg tccctaccgt cccggggtgc gatccgttgc
     4561 gaccggcata gcctgggtct atggcgcacg cgactgtcga gcctgccgct ccgccaccgc
     4621 agaccagacc gccgtccggc ctggccggtg tcgtcggact gggcgtcgcc gtcgccgtga
     4681 tggtggcggc cagcctcact gccctcaccg atgcgcgtcc ggtgacttcg ctgggccttc
     4741 ccgacccggg ggtgttgacc aatgtcggtc tgcctgccgt gcgggcgggc accgaggcgc
     4801 tcatggtcct gacgatcggc gcgcttctgc tcgcggcgtt cctcgtctcg ccgcagcggg
     4861 ccggctacct ggatgtgggc gggtaccggg cgctccgcgc ggcgtcgtgg accgcggccg
     4921 gctgggcggc gggctccata ctgctgatcc cgctgaacgt cgccgatgct ctgggccggc
     4981 ccgtcggcga cgtgctcgat cccggcctgt tgatcgacct gataccccgg ctgtccgcgg
     5041 ccaccgcgtg gtcgttgacc gcgctcgtcg cgctgctcgt gctcgtcggc tgccgcacgg
     5101 tgctcacctg ggggtggagc gcggtgctgt tcggcctggc gctgctcggc ccgttgccgg
     5161 ttgcgctgac cgggcactcc gcgggtggtg gcgcccacga catcgcgagc gacagcctgg
     5221 tgctgcatgt cctggccgcg tcgctgtggg tgggcgggct cgtcgcgctg ctcgggatcg
     5281 ccactacccg gggccccgat cgcagcagcg cactggcgac tgccgtaccc cgcttctccc
     5341 ggctggcact ggcgtgctgg ctcgtgctcg ccgtcacggg tgtggtgaac gcactcgtcc
     5401 gaatcccgct caccgcactc atcgggtccg agtacggcgc cctcgtgatg gcgaaggtgg
     5461 cggcgctgat cgtgctcggt gcgctcggcg ctgcccaccg ccgctcgtcg gtggggccgg
     5521 cgtcccaggg ggacacccgg gcgctcctgc gcctcggcgg ggtcgaggtg ctcctgatgc
     5581 tcgcgacgat cgggctcgcg gtggcgctgg gccgcagcgc tccgccggac acaggcggcg
     5641 tgccgtcccg caccgaggcc gtgatcgggt acgacctctc cgggccgccg acactcgccg
     5701 ggctggcgtt cgactggagg ttcgacctgg tcttcggtac cgccgcgatc gtgctggcca
     5761 tcgcctacct gctgggtgtg cgtcggctgc gccggcgcgg tgacgcctgg gccaccggcc
     5821 gcaccctggc ctggctggtg ggctgcctcg cgctccttgt ggccacgagc tcggggatcg
     5881 gtcggtacgc cccggccatg ttcagcgtgc acatgggtca gcacatgatc ctggggatgc
     5941 tcgtgccgat cctgctcgta ctgggtgggc cggtcacgct cgccctgcgg gtgctcccgc
     6001 cgtccggccg ggccgggccg cctggtccgc gcgagtggtt gctcgccgcg gtgcactctc
     6061 cgatcgcccg ctggctgacg cacccgctcg tggcgctggc cttgttcgtc ggtagctact
     6121 acgtcctgta cttctcggga ctgttcccgg ccgccctgcc ccagcactgg gcgcacaagc
     6181 tgatgaacct gcacttcctg ctggtgggcg cgctcttctt ctggccgatc gtcggcatcg
     6241 acccggcacc gcgccgcctc ccgcccccgg cccgcatggg tctcgtcttc gcgtccgtgc
     6301 ccttccacgc gttcttcggc gtggccgtga tgagtgccaa caccgcgctg ggcggcgagt
     6361 actaccagag tctcgccctg ccgtgggtgc ccgatccgct acatgaccag cacctgggcg
     6421 gtggcctcgc gtgggctgcg ggagaggtgc cgctgctgct cgtggtgatc gcgctgctgg
     6481 tccagtggtc gcgccaggac gaacggtcgg cccgacgcga tgatcgtcgg gcggacgcgg
     6541 acggcgactc ggacctcgag gcatataacg cgatgttgcg ccgcctggcg acgaacggac
     6601 cgccgctcgt cgcgcagagc gacgaatcag ggtatggctc gggtcacaca gaagatgttg
     6661 cccggactac tccgacgccc ccgcatgatc cgtcggacgg cgattcttcc gccgtttcca
     6721 cagctcagag gcatgaccaa agcacagtgg acggtctgag gggcacccga aacgaccgac
     6781 cgctcgtcgc gaggcgcgac cccgatcgcg acgttgagtg acgtccgacc gctcgtttct
     6841 gcaggtcagc gccctagtgg ggggccgatt tgacgtcgtg tcaacccctc gcgtaacttt
     6901 ctcgttgtca gcgagacacc ggacagaacg aaggccctag cggcctcggt caacagggct
     6961 aactgaccgc cccctccact gggggccccc gggattgaac ttccccgggc ggtgttacag
     7021 tgggggaaca cagccccgga gcactgccta gcggtggtgg atggtgtgcg ggtgtctttt
     7081 gagaactcaa cagtgtgtcg agctttatca tgaattggtt ttatgccagt ttgtttttac
     7141 ctgcgacccc gtcgcaggtt tgctag
//
LOCUS       DASRSC010000232         2649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2053291,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000232 DASRSC010000000
VERSION     DASRSC010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053291"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFE39_11375"
     CDS             <1..535
                     /locus_tag="VFE39_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6259241.1"
                     /translation="DLGHNVTAAARSPKPVIAAIDGYCFGVGLEIALSCDIRLATPRS
                     QFALPEMNLGMIPGSGGTQRLARLIGLSRAKFHIMTATRIQAAQALDWGIVAGLQDDR
                     EALYAAVDELAVRMAAFSPAAQRTAKEVLDKGVDGPLYTGIELERKAYAMLRATHDFA
                     EGVAAFTEKRKPEFTGR"
     gene            543..>2649
                     /locus_tag="VFE39_11380"
     CDS             543..>2649
                     /locus_tag="VFE39_11380"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HET6259242.1"
                     /translation="MKPAPFAYVRPGDLDGALAELAGGDAKVLAGGQSLVPVLAMRLG
                     RPATLVDITAVDGLDRIGIEDGALRVGAAVRQRAVEHADLARAVPLLGLALPFVGHRE
                     LRSRGTVCGSLAHADPAAELPAVAACLGAVLEIAGPRGRRTVGAEEFFAGAMTTAVAA
                     DEVLVGAAFPVAHTGEGHGFGEIARRHGDFALAGAVTQVRRGEDGAVLEARLTAFGVA
                     DRPVTRDVTELVRATDGAERPLREAMAEIAAEVVDTAGDTHGSTGYRRRLLTVLAARE
                     LSRAHRAAQPPESPSPESQHPESQHLESQHPEPRHGGGGLRRAGDHGPRRARGRVGAA
                     ELCDVTMRANGSDVTVRVSPRMHLADALREYLGLTGTHLGCEHGVCGMCTVLVDGAAA
                     RACLLFAVQCEGADIITVEGLGSPDEQHPLQQAFSAHHALQCGFCTPGMLMSSYDLLA
                     GDVSDGSVIPEELPERMSGVLCRCTGYRGILAAVADVAAAYPGGVPAPMNCVSRTLVG
                     RGGGPGQGATGPEGPGHEAPVEAGEPAGEVAIPAGSPSATVEVTSALTAPVDAVWAVL
                     DDVERMVACLPGAELTESLGGDRYRGTAGVALGPVKLSFAGLAQIIERVPADRRLRMI
                     GQGSDAGGSATQADIRLRAEPGWGGGTVLRADADLYLSGRVAQFGRALAGDVSRRLFE
                     QFATAVDAAAAGREAPARRR"
BASE COUNT          330 a          951 c         1005 g          363 t
ORIGIN      
        1 cgacctcgga cacaacgtca ccgccgcagc gcgcagcccg aaaccggtga tcgcggcgat
       61 cgacgggtac tgcttcggcg tcgggctgga gatcgccctg tcctgtgaca tccggcttgc
      121 cacgccacgc agccagttcg ccctgcccga gatgaacctc ggcatgatcc cgggatcggg
      181 cggcacccag cggctcgccc ggctgatcgg gctctcccgc gcgaagttcc acatcatgac
      241 cgccacgcgg atccaggcag cgcaggcgct ggactggggg atcgtggccg gcctgcagga
      301 tgaccgcgag gccctgtacg cggccgtcga tgagttggcg gtacggatgg cggccttctc
      361 cccggccgcc cagcgcacgg cgaaggaggt gctggacaag ggcgtggacg gaccgctcta
      421 cacgggtatc gagctggagc ggaaggccta cgccatgctg cgtgccacgc acgacttcgc
      481 ggagggggtc gccgcgttca cggagaagcg caagccggag ttcaccggac ggtgaggctg
      541 tcatgaaacc ggcaccgttc gcctacgtcc gacctggcga cctcgacggc gcgctcgccg
      601 agctcgcggg cggggacgcc aaggtgctcg ccggcggcca gtcgctcgtc ccggtgctcg
      661 cgatgcgctt gggccgcccc gccacgctcg tcgacatcac ggccgtcgac gggctcgacc
      721 ggatcggcat cgaggacggc gccctccggg tcggggcggc ggtccggcag cgcgcagtgg
      781 agcacgctga cctcgcccgc gccgttccgc tgctcgggct ggcgctgccc ttcgtcgggc
      841 accgggagct gcgcagccgc ggtaccgtct gcggaagtct cgcccacgcc gacccggccg
      901 ccgagctccc cgccgtggcg gcttgcctcg gtgccgtgct ggagatcgcc ggcccccggg
      961 gacggcgcac cgtcggtgcc gaggagttct tcgccggggc gatgacgacc gccgtggcag
     1021 ccgacgaggt gctggtcggc gcggcgtttc cggtcgcaca cacgggagag ggccacggct
     1081 tcggcgagat cgcccgccgc cacggcgact tcgcgctggc cggggcggtc acgcaggtgc
     1141 gtcggggcga ggacggggcc gtgctcgagg ctcggctcac cgcgttcggc gtggccgacc
     1201 gtccggtgac ccgcgacgtc accgagctcg tccgggcgac cgacggtgcg gagcggccac
     1261 tgcgcgaggc gatggccgag atcgccgcgg aggtggtcga cacggcgggc gacacgcacg
     1321 gctcgaccgg ctaccgccgc cgcctcctga cggtcctggc cgcgcgcgaa ctgtcccgcg
     1381 cgcatcgtgc cgcccaaccc cctgaatctc catcccccga gtcccagcac cccgagtccc
     1441 agcacctcga gtcccagcac cccgagcccc gccacggtgg cggtggtctc cggcgggccg
     1501 gagaccacgg tccgcgtcgt gcacgcgggc gggtgggggc ggcggagctc tgcgacgtca
     1561 cgatgcgagc gaacgggagc gacgtcactg tgcgggtgtc gccgcgcatg caccttgccg
     1621 acgcgttgcg ggagtacctc ggcctcaccg gcacccacct cggatgcgag cacggcgtgt
     1681 gcggcatgtg taccgtcctc gtcgacgggg cagccgccag ggcgtgcctg ctgttcgcgg
     1741 tgcagtgcga gggtgccgac atcatcaccg tcgagggcct cggcagtccc gatgagcagc
     1801 acccgttgca gcaggcgttc tccgcgcacc acgcgctcca gtgcggcttc tgtacgcccg
     1861 gcatgctcat gagctcctac gacctgctcg caggtgacgt gtccgacggg tccgtcattc
     1921 cggaggagct gccggagcgg atgtccgggg tgctctgccg ctgcaccggc taccgcggga
     1981 tcctggcagc cgtggcggat gtcgccgccg cgtaccccgg cggcgtgccg gcgccgatga
     2041 actgcgtgtc ccgcacgttg gtcgggcgcg gcggcgggcc gggccagggg gcgaccgggc
     2101 cggagggtcc tggacatgag gcgcccgtcg aggcaggtga gcccgcgggt gaggttgcga
     2161 tcccggccgg gtccccgtcc gcgacggtcg aggtgaccag cgcgctcacc gctccggtcg
     2221 acgccgtgtg ggcggtgctc gacgacgtcg agcggatggt ggcctgcctg cccggtgccg
     2281 agctcaccga gtcgctcggc ggcgaccgtt accggggcac ggccggggtg gcactcgggc
     2341 cggtgaagct gtcgttcgca gggctcgcac agatcatcga gcgggtcccg gccgaccgtc
     2401 ggctccggat gatcggccag ggttccgatg cgggcggcag cgcgacgcag gccgacatcc
     2461 ggctgcgggc cgagcccggt tggggcggtg gcacggttct gcgcgcggac gccgacctct
     2521 acctgtcggg ccgcgtcgcg cagttcgggc gcgcgctggc gggggacgtc agcaggcggt
     2581 tgttcgagca gttcgccacg gccgtcgacg ctgccgcggc aggacgagag gcgcccgccc
     2641 ggcgtcgcc
//
LOCUS       DASRSC010000233         3951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1071105,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000233 DASRSC010000000
VERSION     DASRSC010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071105"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VFE39_11385"
     CDS             <1..557
                     /locus_tag="VFE39_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="membrane protease subunit"
                     /protein_id="HET6259243.1"
                     /translation="VHIDRVEIKDVALPETMKRSMSRQAEAERERRSRVITAEGELQA
                     SQKLAQAAEVMAEHPAALQLRLLQTVVEVAAEKNSTLVLPFPVELLRFLERSTPSEAA
                     EASQATTPKVPELTEPTSPELSSAPEPATAAFSTPQAVPSSTPEPVNSSTPETVNSSE
                     PEAAPSPNPATVAASRPAAVASSN"
     gene            659..2086
                     /locus_tag="VFE39_11390"
     CDS             659..2086
                     /locus_tag="VFE39_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET6259244.1"
                     /translation="MAHSPADQPATLTASAGLRDLMQYVADHVAGYRERVGDGPAFPS
                     TVDLDDVRAALGSLPDEPTPAAAVVEQLADAVEPALVASTGPRYFGFVIGGVLDAATA
                     AEVLTTGWDQNAFNPLTSPAGAVVEEVAGAWLTDLLGLPVGVSFGFVTGGQGGNTVCL
                     AAARHGVLSDAGWDVERRGLHGAPPVRVVAGTGRHATIDRALRLLGLGTDALVPVRTS
                     LQGSIDVEDLGRALASTSVDPTIVCLQAGEVNTGAFDDFPAAIASAHQHGAWVHVDGA
                     IGLWAAASPATRHLVAGVERADSWSTDGHKWLNVPHDAGYAFCAHPATHVAATSYSAA
                     YLTGQGSDALRGPGDYVLESSRRARGFTTWAALRQLGRSGVADLVDRCCALARRFAHQ
                     LATVDGVTVVNDVVLNQVLVRFGDDEQTNRVIDAVQRSGECWMGGTTWHGMRLMRISV
                     SSWRTTEADVDRSVAAIVTALEQRS"
     gene            complement(2112..3377)
                     /locus_tag="VFE39_11395"
     CDS             complement(2112..3377)
                     /locus_tag="VFE39_11395"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HET6259245.1"
                     /translation="MMEQFAAARPGVGALLRSLDEAPPTDLVDVCSGWLARRVSARAC
                     VLLLADYAEASLEPVTRGPGAARIQPQDLALSAAGEAYREQRSITAALDHPGEAPAAG
                     AAPGGTGGPELVVYLPVSVRAERLGVLAVTLPGHDLGREVEEVLEDVARVLGHVLTGA
                     RRYTDRFEMLRRRRELGLAAEIQWELLPSLAFELPGFSIAGTVEPAYEIGGDNFDYAV
                     SAHRLTVAVSDAAGHGLRAALLASLSVTAMRNARRAHCSIVEQADAANRYLVEQFPGP
                     DFVTGLVLELDVDSGEGMLLNAGHPPPLLLRDGAVSEFVVAPALPLGLTPSARYPLHP
                     FRLEPGDRLLLFTDGITEAHQRGEREFGYRRLGAMLAEHHDLTPPEFVRHVTRAVTES
                     CNGELNDDATAVCLDWHPAPVVAAHRVGG"
     gene            3598..3915
                     /locus_tag="VFE39_11400"
     CDS             3598..3915
                     /locus_tag="VFE39_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259246.1"
                     /translation="MVPDEVDLGAAAAVPVAGVTALRALRRLGFLTGRRVLITGASGG
                     VGGYAVQLAASAGAHVIASVGSAARGEGLVMFALVGGPFRAENGCGCPLTPLLYGPFE
                     VVV"
BASE COUNT          551 a         1464 c         1400 g          536 t
ORIGIN      
        1 gcgtgcacat cgaccgcgtc gagatcaagg acgtcgcgct acccgagacg atgaaacggt
       61 cgatgtcacg ccaggccgaa gccgaacgcg agcgacggtc ccgcgtgatc accgccgagg
      121 gcgagctgca ggcctcacag aaactggccc aggccgccga ggtgatggcc gagcatcccg
      181 cagcgctgca gctgcgcctc ctgcagaccg tcgtcgaggt cgccgccgag aagaactcca
      241 ccctcgtgct gcccttcccc gtcgagctgc tccgcttcct cgaacgatcg acaccgtccg
      301 aggcggcgga ggcctcccag gcgaccacac ccaaggtccc cgagctcacc gagccgacct
      361 ctccggagct gtcgtcggcg ccggaaccgg ccacggcggc cttctcgacg ccgcaagccg
      421 tgccctcgtc aacaccggaa cccgtgaact cgtcgacacc ggaaaccgtg aactcgtcgg
      481 agccggaagc cgcgccctcg ccgaacccgg cgacggtggc cgcgtcgagg cctgcggccg
      541 tggcctcgtc gaactagcct gattcgcggt cgcgtgccgc gagttgcgcc cccggcagag
      601 ggagcacgtc ggcgagccgc atgtcggtgc acgacccggg ctgcggcatg gtcgacggat
      661 ggctcactcc cccgccgacc agccggccac cctgaccgcg tccgcaggcc tgcgcgacct
      721 gatgcagtac gtggccgacc acgtggccgg ctaccgggag cgggtcggcg acgggccggc
      781 ctttccctcc acggtcgacc tcgacgacgt ccgtgccgcg ctcggctcgc tgcccgacga
      841 gccgaccccg gcggccgcag tggtcgagca actggcggac gcggtcgagc cggctctggt
      901 ggccagcacc ggcccgcgct acttcgggtt cgtgatcgga ggggtgctcg acgccgcgac
      961 ggcggcggaa gtactgacca cggggtggga ccagaacgcg ttcaacccgc tgacctcccc
     1021 cgccggggcc gtggtcgagg aggtggccgg cgcctggctc actgacctcc tcggccttcc
     1081 cgtcggggtc tcgttcggct tcgtgaccgg cggccagggc ggcaacacag tctgtctcgc
     1141 cgccgcccgg cacggcgtgc tgtccgacgc gggctgggac gtcgaacgcc ggggtctgca
     1201 cggcgcgcca ccggtccggg tggtggccgg cacggggcgg cacgccacca tcgacagggc
     1261 actacgcctg ctggggctgg gaaccgatgc tctggtcccg gtgcggacca gcctgcaggg
     1321 ctcgatcgac gtcgaggatc tcggacgggc gctggccagc acgagcgtgg acccgaccat
     1381 cgtctgtctg caggccggtg aggtgaacac cggcgcgttc gacgacttcc ccgcggcgat
     1441 cgcaagcgcg caccagcacg gcgcatgggt ccacgtcgac ggggcgatcg ggctgtgggc
     1501 cgccgccagc ccggccaccc gccacctcgt ggcgggcgtg gaacgggccg actcgtggtc
     1561 cacggacggc cataagtggc tcaacgttcc ccacgacgcc ggctacgcgt tctgcgccca
     1621 tccggccacg cacgtcgcag cgacctccta cagcgccgcc tacctcaccg gccagggttc
     1681 cgacgcgctg cgggggcccg gcgactacgt gctggagtcc tcccgccggg cccgcggctt
     1741 cacgacgtgg gccgcgctgc gtcagctcgg ccggtccgga gtcgccgacc tggtcgaccg
     1801 ctgctgcgcg ctcgcccgcc ggttcgccca ccagctcgcc accgtcgacg gagtaacggt
     1861 cgtcaacgac gtcgtgctca accaggtgct cgtccgcttc ggcgacgacg agcagaccaa
     1921 ccgggtgatc gacgccgtcc agcgctccgg cgagtgctgg atgggcggca cgacctggca
     1981 cggcatgcgc ctgatgcgca tctcggtgtc gagctggcgc accacagagg ccgacgtcga
     2041 tcgctccgtc gccgccatcg tgacggcact cgagcagcgg tcctgaccgg cgggctcgac
     2101 gcgctagccg ctcagcctcc tactcggtgc gcagcgacca ccggggcggg gtgccagtcg
     2161 aggcagaccg cagtggcgtc gtcgttgagc tcgccgttgc aggactcggt gacggcgcgg
     2221 gtgacgtgcc ggacgaactc ggggggcgtg aggtcgtggt gttcggcgag catggcgccg
     2281 aggcggcgat aaccgaactc ccgctcgccg cgctggtgcg cctccgtgat gccgtcggtg
     2341 aacaacagca gtcggtcacc gggctccaac cggaacgggt gcagcgggta gcgagccgac
     2401 ggcgtgagcc ccagcggcag cgcgggcgca acgacgaact cgctcaccgc accgtcgcgc
     2461 agcagcagcg gcggtggatg ccccgcgttg agcagcatcc cctcgccgct gtcgacgtcg
     2521 agctccagga ccagcccggt cacgaaatcc ggcccgggaa actgctccac caggtaccgg
     2581 ttggccgcgt cggcctgctc cacgatcgag cagtgcgcgc ggcgggcgtt gcgcatcgcg
     2641 gtgaccgaca ggctggccag cagcgcggcc cgcagtccgt gaccggccgc atcgctgacc
     2701 gccaccgtca accggtgcgc gctgaccgca tagtcgaagt tgtcgccccc gatctcgtac
     2761 gccggctcca ccgtgccggc gatggagaac ccgggaagct cgaaggccag gctcggcagc
     2821 aactcccatt ggatctccgc ggccaacccc agctcacgac gccgccgcag catctcgaac
     2881 cggtcggtgt agcggcgcgc cccggtcagg acatgcccca acacccgcgc cacgtcctcg
     2941 agaacctcct ccacctcacg gcccaggtcg tgacccggca gggtgacagc cagtaccccg
     3001 agtcgctcgg cgcgcaccga caccgggaga tagaccacca gctccggtcc acctgtccca
     3061 ccgggcgcgg cccccgccgc cggggcctca ccggggtggt ccagagcggc ggtgatgctg
     3121 cgctgctcgc ggtaggcctc cccggcggca ctgagggcca ggtcctgtgg ctggatccgg
     3181 gcggcccccg ggccgcgtgt gaccggttcc aggctcgcct ctgcgtagtc ggccagcagc
     3241 agaacacagg cccgggcgga cacgcgccgg gccagccagc ccgagcagac gtccaccaga
     3301 tccgtggggg gcgcttcgtc caggctccgc aacagcgcgc ccaccccggg ccgagccgcg
     3361 gcgaactgct ccatcatcgc gcccaccctg ccaccacgtg cgtcgcgcgt gtcagaccgg
     3421 gcgtggcgga tcaggactcc ttcgtggagt agccgggcgt gcactctcca gccacgcccg
     3481 gcgccgctcg gtgagcggac ggaagtcgga caggcgcccg gacaggtcca ccgcctcacc
     3541 ttcgggccgg gcggtgcgtg ggcgcagctg cgggcagtcc cggctgccga gctcgcggtg
     3601 gttccggacg aggtcgatct cggagcggcc gcggccgtcc ccgtcgcggg ggtgacggcg
     3661 ctgcgtgcgc tgcgcagact cggattcctg accggtcgcc gcgtgctgat caccggggcg
     3721 tcaggtgggg tgggcgggta cgcggtgcag ctggctgcgt ccgccggtgc gcacgtgatc
     3781 gcctcggtcg gcagcgcagc gcgcggtgag ggtctagtca tgttcgccct ggtgggcggc
     3841 ccgttccggg ctgaaaatgg gtgtggttgc ccgctgacgc cgttgctcta tggccccttc
     3901 gaggtcgtgg tctagtcggg cgctgggagc tgccccgtgg gcccttcagt g
//
LOCUS       DASRSC010000234        12846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3688830,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000234 DASRSC010000000
VERSION     DASRSC010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12846
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3688830"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /locus_tag="VFE39_11405"
     CDS             complement(<1..1135)
                     /locus_tag="VFE39_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259247.1"
                     /translation="MARRARRFAYGPYTNGPDPLAPPVDLRAAMDEIGRDVMEGSSPR
                     SALQELLRRGMENRRGLDELTREVWQRRSRLQRENRLDGTLQEVRELLQRALDAERDS
                     LGRQDTDDARFREMQLDALPSDTGGAVRELDSYDWQSPDAREAYEEIRDLLGREMLDQ
                     RFAGMKQAMENATPEDVQRIREMLDDLNDLLANHATGQDTTEQFQQFMQQHGEFFPEN
                     PQNTEELVDLLAQRAAAAQRMLNSMTPEQRAELMELSQQAFGDPRLAQALAQLDAQLQ
                     GLRPGEDWDGQGRFRGDNPMGMGEATRAMEQLGQLDALAEQLSQSYPGARMEDIDLDA
                     LGDHLGDKARVDARALAELERELQRQGLFERAPDGSLQLSPKAL"
     gene            complement(1217..2605)
                     /locus_tag="VFE39_11410"
     CDS             complement(1217..2605)
                     /locus_tag="VFE39_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6259248.1"
                     /translation="MPHDLPRTLGALRASGHEMRGVKDEIRQNLLTALREGRDPWPGI
                     VGFESTVLPQLERALIAGHDVVLLGERGQGKTRLLRSIVNLLDELTPVIEGSELGEHP
                     YEPITPASRRRAAELGDDLPVAWRHRDERYTEKLATPDTAVADLIGDVDPVKVAEGRS
                     LGDPETIHYGLVPRAHRGIVAINELPDLAERIQVSLLNVMEERDIQVRGYTLRLPLDV
                     LLVASANPEDYTNRGRIITPLKDRFGAEVRTHYPLELADEIALVQQEAHLAAEVPRHL
                     IEVVARFTRSLRESSAVDQGSGVSARFAVAAAETVAAAALRRAAITGEERAVARPVDL
                     ESVPAVLRGKLEFASGEEGRESEHLEHLLRRATADTARARLRGIDLNPLAEAVASSPV
                     RTGERVAAVEVVAALPAVDALTEVAKRLDAGGTEDPGPMASAAELALELLFLTRRLAK
                     DESDDGDTVSYG"
     gene            2735..3718
                     /locus_tag="VFE39_11415"
     CDS             2735..3718
                     /locus_tag="VFE39_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6259249.1"
                     /translation="MKAIRQYEFGAADTLRFEDVADPVPGDGQVLIEVAAAGVHLLDT
                     IIRAGMTGGPFPPPTLPMTPGREVAGVVGGVGPGVDGSWAGRSVVAHLGMASGGYAAL
                     AVAPAAALHAVPDAIAPETAVAMIGTGRTALGILDAAELAADDVVLIPAAAGGLGALF
                     VQAARAAGATAVGLAGGPDKVEQVRALGADVAVDYREPDWADQVQAALGDRKPTLLLD
                     GVGGAVGRTALTMLDIGGRIVMFGGSSGAVTEFSSADVVAGGLSVVWVTGPRMLRRYG
                     GLRPLETRALAEAAAGRLVPVVHPPFPLADAAAAHIALEGRATTGKVVLTP"
     gene            3891..4946
                     /locus_tag="VFE39_11420"
     CDS             3891..4946
                     /locus_tag="VFE39_11420"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET6259250.1"
                     /translation="MTTDTIRATCEPRAGSDFARLSRQIAAAGLLDRKPGRYAVRFAL
                     VGAAVAATTTAFVLLGNSPWQLIVAAAAAVVYGQIALLAHDVAHRQVFRTRRAASITG
                     RLLGNLGIGMSYGWWMDKHTRHHANPNHEGLDPDVDAGALVWTGEQAAAGRGLTRFVN
                     RHQAVLFFPLLTLAGLDLRKSSVQGLLGERGAAVRGRRLELALLAAHAVGYLTALLLV
                     LPPGLAVAFFVIHQGLFGVYLGSIFAPNHKGMAMPQGRMDFLRKQVITSRNVRGTTSL
                     TDGLLHVAMGGLNHQIEHHLFPSMPTPNLRRARPVVREFCAEIGVPYHETGLLRSWAE
                     SLGQLRRASAPLRGGAE"
     gene            4995..5897
                     /locus_tag="VFE39_11425"
     CDS             4995..5897
                     /locus_tag="VFE39_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259251.1"
                     /translation="MMLKPATIDLPAAVDFMATHARPLDRHRLRLLLGTGAPEPVLAA
                     TEAYRNADGGYGWGLEPDLRSGTSQPGAALHAFEAFEDIAPMTTTRAGALCDWLESVS
                     LPAGGLPFALPVNDPAGTARWWAQADAGASSLQITAVVTAAALRVARHDPVVAAHPWT
                     ARATRYCLDAVRAVEERPHALVLAFALQFLDAAHDSEPEAAALLDRLATHLPDDGILP
                     VDGGTESEVIRPLDIAPAPDRPVRSLFAPDVVVADLHRLGGTQQDDGGWRVEFAEFSP
                     AGALDWRGHATVRAVAVLRANGVI"
     gene            complement(5913..6818)
                     /locus_tag="VFE39_11430"
     CDS             complement(5913..6818)
                     /locus_tag="VFE39_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HET6259252.1"
                     /translation="MTLTAFGLLVLGVLLAEPVSRALAAAHWPARDPVGALLVWQAVG
                     LAGGVALLGSGVMYGLSPLGPSPSVAASVAVGALAVGEVPPALTVTHFAALLAALALA
                     LRLVSVLAVVTLRTLRVRRRHRDLLDLLGTPWPAVPGARVLDHPIPVAYCLPGLRSRL
                     VLSAGVLDALEPAAVRAVLAHEQAHLRERHDLVVLPFVAWGATAPFVRGMVCAQLAVA
                     ALIEMRADDVASAEVTANQLTGALRTMGGAAPAAALSSFTDGLDHRLDRITTPPPPLP
                     GAVRVLVRLAAAGLVAVPTALLLVP"
     gene            complement(6834..7175)
                     /locus_tag="VFE39_11435"
     CDS             complement(6834..7175)
                     /locus_tag="VFE39_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HET6259253.1"
                     /translation="MPTLGELERAVMETLWSAAGPVTARDVQDALGSRDLATTTVLTV
                     LGRLERKGLVTREREGRAHRYRSVASREDHVAELMRDALDAAPDRGAALARFLGSIPA
                     DERATLRELLG"
     gene            7528..9012
                     /locus_tag="VFE39_11440"
     CDS             7528..9012
                     /locus_tag="VFE39_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259254.1"
                     /translation="MLLTTVLGSGLVMIDSTVVNVALAHIGTEFGAGFIALQWTVNAY
                     TLTLASLILLGGSLGDHFGRRRIFLIGVVWFALASLMCGLAPNIETLIAARALQGVGG
                     ALLTPGSLALISASFHGPDRAAAVGAWSGLGGIAGAVGPFLGGWLVEWNWRAVFLINL
                     PLAVLIVAVAVRHVPESRDTESAAGLDIAGTVLAVLGLAGLTYSLTGLSARGAAPDLV
                     AGLIVGVVALVAFVLWERRSPHPLVPPELFRNTTFSATNAVTLLIYGALGVVFLLLVL
                     QLQIVVGFSPLAAGTSLLPVTLIMLAFSARAGALAGRIGPRWPLTLGPLISAAGLLLM
                     LRIGPGASWLTDVLPAVLVFGAGLALTVAPLTATVLDSAPDRYAGAASGVNNAVARAA
                     GLLAVAVIPGIAGIRGDDYTDPASFGTGFRIAMVIGAGLLVAASLLAFVVVRRPLRAA
                     EPDAAEPDAADTGAPERVRIEECMHCPVTGPQVLPPPDRALGNR"
     gene            complement(9062..9898)
                     /locus_tag="VFE39_11445"
     CDS             complement(9062..9898)
                     /locus_tag="VFE39_11445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET6259255.1"
                     /translation="MTVELGRYGVWRHVGGLTPELAAEVEALGYGAIWIGGSPPGDLR
                     LAESLLDATERIAVATGIVNMWTTPAEEAAESYHRIEAAHPGRFLLGVGIGHPEATSD
                     YRRPYATIVEYLDRLDAAKVPVDGRVLAALGPKVLKLAAERTAGAHPYLTTPEHTREA
                     RELLGEGVLLAPEHKVVLDTDPERARAIGRPVVAKPYLGLRNYTSNLLRLGWSEEDLA
                     DGGSDRLIDALVAHGDAAAVAARLTEHLDAGADHVCAQVLPGGDDPVPALRELAEALN
                     LR"
     gene            9968..11602
                     /locus_tag="VFE39_11450"
     CDS             9968..11602
                     /locus_tag="VFE39_11450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6259256.1"
                     /translation="MSREPTTRAAPLRMRPRVVVPPDPRQPREAPAGTEAVVVGGGVA
                     GISAAVVLAERGVAVTLLEAAPTLGGRLGAWPHTLPDGTEQVVEHGFHAFFRHYYTWR
                     AVLRRIDPALSFLRPVDGYPVISRQWPPEDLTGLPAAPPLNLLALFARSPSLGLRDLL
                     GSRQSLAAELLAYDRERTTAAFDTVPAAEFLAALGMSDRARAMLFEAFARSFFAEPGA
                     LSAAELIAMFHYYFLGNPEGIGFDAPATDYLTCIWEPFRAFLVRRGAEIRTSTAARAI
                     LPADGHRWRVELADAAPLETRHLVLATEPGALRSLLAASPAAAAVAPRLAAKTAALTV
                     APPFAVTRLWLDRDVAPERSTFSAVSREPTLDSVTLYSRLERPSAAWAARTGGAVVEL
                     HSYACAAPDADTATARMRAELAALWPETAAAGLVHLQQRLEATAPTFPPGGAGTRPTV
                     SGEARGIRVAGDFVETPFLTGLMERASASGVLAANDVLAEVGAGPEEILGGPQRGLLA
                     GLLPARATPRWSTPPSSIPGRVRVEPAAAPSAARRR"
     gene            11599..>12846
                     /locus_tag="VFE39_11455"
     CDS             11599..>12846
                     /locus_tag="VFE39_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3'-5' exonuclease/DNA polymerase"
                     /protein_id="HET6259257.1"
                     /translation="MRIALDPGPRDGPVRIRRLADDGAPLGSEETLTDLARVAELERE
                     HGPRWVFARAHTYRRLLAAGVEVARAHDVELVEGLLVGAAGHYGQPHGLAAATARLHG
                     RTPDPDPPAGDDGPPALFETGPATDELTDVVAVHAAQQRALAAAGPGMRLLAAAESAG
                     VLVAEEMTCYGLPWRAEIHDQLLTELLGPRPVLGGRPPKLAALAEDVTTAFDGRPLNP
                     DSPAEVLRAFARAGHELETTRSWELRRIDHPAVEPLLAYKELARLHSAHGWSWLRTWV
                     DGGRFRPEYVVGGVVSGRWASRGGGALQIPRAVRAAVRADPGWRLVVADAAQLEPRVL
                     AALSGDHALAPGEDDLYTALAAREFEGDRARAKLALLSAMYGQTSGGAGVLLATLRRR
                     FPAAMAYVEDAARAGEEGRLVHSH"
BASE COUNT         1623 a         4733 c         4665 g         1825 t
ORIGIN      
        1 gcaacgcctt gggcgacagc tgcagcgacc cgtccggcgc ccgctcgaac aggccctgac
       61 gctgcaactc gcgttccagc tcggccaggg cgcgagcgtc gacccttgcc ttgtcgccca
      121 ggtggtcgcc gagcgcgtcg aggtcgatgt cctccatccg cgcacccgga tagctctgcg
      181 agagctgctc ggcgagcgca tcgagctgcc cgagctgctc catcgcgcgg gtcgcctcgc
      241 ccatgcccat cgggttgtcg ccgcggaatc ggccctgccc gtcccagtcc tcgcctggcc
      301 gcaggccctg cagctgggcg tcgagctggg ccagcgcctg cgccagccgt gggtcgccga
      361 aggcctgctg ggacagctcc atcagctcgg cccgctgctc gggcgtcatc gagttgagca
      421 tccgctgggc cgccgccgca cgctgcgcga gcaggtcgac gagctcctcg gtgttctgcg
      481 ggttctccgg gaagaactcg ccgtgctgct gcatgaactg ctggaactgc tccgtggtgt
      541 cctgcccggt ggcgtggttc gcgagcaggt cgttgaggtc gtcgagcatc tcccggatgc
      601 gctggacgtc ctccggggtg gcgttctcca tcgcctgctt catgcccgcg aagcgctggt
      661 cgagcatctc ccggccgagc aggtcgcgga tctcctcgta ggcctccctg gcgtccgggg
      721 actgccagtc gtaggagtcc agctcgcgga ccgcgccgcc ggtgtcggac ggcagcgcgt
      781 cgagctgcat ctcccggaac cgggcgtcgt cggtgtcctg ccgtccgagc gagtcgcgct
      841 cggcgtcgag agcacgctgc aggagctcgc ggacctcctg cagcgtgccg tcgagccggt
      901 tctcccgctg cagcctgctg cgccgctgcc agacctcgcg ggtgagctcg tcgagcccgc
      961 gccggttctc catgccgcgg cgcaacagct cctgcagcgc ggagcgcggc gaggagccct
     1021 ccatgacatc gcgaccgatc tcgtccatcg cggccctgag atcaaccggt ggagcgagcg
     1081 gatcgggccc gttggtgtag ggcccgtagg cgaacctacg tgcgcgtcga gccacggcac
     1141 ctcctgtgcg cttgcacgaa cggcacgctg gtccggtagg tccgcacgaa tgtgccgctc
     1201 gtcgcgtcga tcgctgtcat ccgtagctca cggtgtcgcc gtcgtctgac tcgtcctttg
     1261 ccaggcgcct cgtgaggaac agcagctcta gcgccagttc cgccgcgctc gccatcgggc
     1321 ccgggtcctc cgtgccgccg gcgtccagcc gcttcgccac ctcggtgagg gcgtccacag
     1381 cgggcagcgc ggccaccacc tcgacggccg ccacgcgctc cccggtgcgc accggggagc
     1441 tggcgacggc ctcggcgagc gggttgaggt cgatgccgcg cagccgtgcg cgtgcggtgt
     1501 cggccgtggc ccggcggagc aggtgctcca ggtgctcgga ctcgcggccc tcctcgccgg
     1561 aggcgaactc cagcttgccg cgcagcacgg caggcactga ctccaggtcg acgggacgcg
     1621 cgacggcacg ttcctcgcct gtgatcgcgg cccgccgcag cgcagcagcg gccacggtct
     1681 cggcggcggc cacggcgaac cgcgccgaca cccctgagcc ctggtcgacg gcgctggact
     1741 cgcgcagcga ccgcgtgaac cgggcgacga cctcgatcag atgccgcggc acctcggccg
     1801 cgaggtgtgc ctcctgctgg accagcgcga tctcgtcagc cagctccaac gggtagtggg
     1861 tgcggacctc ggcgccgaag cggtccttga gcggggtgat gatccgcccg cgattggtgt
     1921 agtcctcggg gttggccgac gcgacgagca gcacgtccag cggcaggcgc agggtatagc
     1981 cgcgcacctg gatgtcgcgc tcctccatca cgttcagcag cgacacctgg atgcgttcgg
     2041 ccaggtcggg cagctcgttg atcgcgacga tcccgcggtg cgcgcggggt acgaggccgt
     2101 agtggatcgt ctccgggtcg cccagggagc ggccctcggc gaccttgacg gggtcgacgt
     2161 cgccgatgag gtcggcgacg gcggtgtcgg gggtggcgag cttctcggtg tagcgctcgt
     2221 cgcggtgccg ccaggccaca ggcaggtcgt cgcccagctc ggcggcgcgc cggcgggagg
     2281 cgggagtgat gggctcgtag ggatgctcgc cgagctcgga gccctcgatc acgggggtca
     2341 gctcgtcgag caggttgacg atggaccgca gcaggcgcgt cttgccctgg ccacgctcgc
     2401 ccaacagcac gacgtcgtgg ccggcgatga gcgcgcgttc cagctgcggg agcacggtcg
     2461 actcgaaccc gacgatgccg ggccatgggt cgcgcccctc gcggagtgcg gtgaggaggt
     2521 tctggcggat ctcgtccttg acaccacgca tctcgtggcc ggacgctcgg agggcaccga
     2581 gggtgcgggg aagatcatgg ggcacgtaat caacgttacg ccgggatccc cctttcgtgc
     2641 gcggcgtcgc cgctccggaa cggttgttga cctctacctg acttcagctc ctagcgtgat
     2701 cgcagcaggt cggcgacagg tgagggagac gcgagtgaag gcgatccggc agtacgagtt
     2761 cggggcggcg gacaccctgc ggttcgagga cgtagccgac cccgtaccgg gcgatgggca
     2821 ggtgctcatc gaggtcgccg ccgcaggcgt gcacctcctc gacacgatca tccgggccgg
     2881 catgacgggc ggcccgttcc cgccaccgac gctgcccatg acgccgggtc gcgaggtggc
     2941 gggcgtcgtc gggggcgtcg gtcccggcgt cgacgggagc tgggccgggc gcagcgtcgt
     3001 cgcgcacctc gggatggcca gcggggggta cgccgcattg gcggtggctc cggccgcggc
     3061 gctgcacgcc gtacccgacg cgatcgcgcc ggagacggcc gtcgccatga tcggcaccgg
     3121 tcgtacggcg ctcggcatcc tcgacgccgc ggagctcgca gccgacgatg tcgtgctcat
     3181 ccccgcggcg gccggcgggc tgggggcgct gttcgtccag gcggcacggg ccgcgggcgc
     3241 gaccgccgtc gggctggccg gcggaccgga caaggtggaa caggtgcggg cgctcggcgc
     3301 cgacgtcgcc gtcgactacc gggagccgga ctgggccgac caggtccaag cggccctcgg
     3361 cgaccggaag ccgacacttc tcctggacgg cgtcggcggt gcggtcggcc ggacggcgct
     3421 gacgatgctc gacatcggtg ggcggatcgt catgttcggc gggtcgtccg gcgcggtcac
     3481 cgagttcagc agcgccgacg tcgtcgcagg cgggctgtcg gtcgtgtggg tcaccggacc
     3541 gcgcatgctg cgccgctacg gcggcctgcg cccactggag acccgagcgc tggccgaggc
     3601 tgcggccggc cggctggtac cggtggtgca cccgccgttc ccgctcgccg acgcggcggc
     3661 cgcccacatc gctctggagg gtcgcgcgac gacgggcaag gtggtcctga cgccctgagg
     3721 aggcgccctg cgcaggaatg cgctcctggc cggcaggccc gaccgcgtgg cggccacgtc
     3781 gcgctgacgg cgcctgtcgg aacaggggcc tcggagggga gggttgcccc accccgatcc
     3841 ggggggcgca ccggctcaac ccgggtgtcg gattcccgca ggctcgcccc atgaccaccg
     3901 acaccatccg ggccacctgc gagccacgag ccgggagcga cttcgcgcgc ctgtcgcggc
     3961 agatcgccgc cgccgggctg ctggaccgca agcccggccg ctatgccgtc cgcttcgccc
     4021 tcgtcggcgc ggccgttgcc gcgacgacca ccgcgttcgt cctgctcggg aactcaccat
     4081 ggcagctgat cgtcgccgct gccgccgccg tggtgtacgg ccagattgcg ctgctcgcgc
     4141 acgacgtcgc gcaccgacag gtcttccgga cccgccgcgc cgcctcgatc actgggaggt
     4201 tgctcggcaa cctcggcatc ggcatgtcct acggctggtg gatggacaag cacacgcgcc
     4261 accacgcgaa ccccaaccac gaagggctcg accccgacgt cgatgcgggc gcgctcgtct
     4321 ggaccggcga gcaggcggcg gcgggccggg gcctcacccg attcgtgaac cgccaccagg
     4381 cggtgctgtt cttccccctc ctcacgctgg ccggactcga cctgcgcaag agcagcgtcc
     4441 agggtctgct gggcgagcgc ggcgccgcgg tacgcggccg ccggctggag ctggcgttgc
     4501 tcgccgccca tgccgtcggg tacctgacgg cactgttgct ggtactgcca ccgggcctcg
     4561 cggtcgcctt cttcgtgatc caccagggcc tgttcggggt gtacctcgga tcgatcttcg
     4621 ctcccaacca caagggcatg gcgatgccgc agggccgcat ggacttcctg cgcaagcagg
     4681 tgatcacatc gcggaacgtg cggggcacca cgtccctgac cgacggcctg ctgcacgtgg
     4741 cgatgggtgg actcaaccac cagatcgagc atcacctctt cccgagcatg ccgactccga
     4801 acctgcggcg agcgcgcccg gtggtgcggg agttctgcgc cgagatcggc gtgccctacc
     4861 acgagacggg cctgctccgc tcctgggcgg agtcgctggg acagctgcgc cgcgccagtg
     4921 cgccgttgcg cggaggggcc gagtgaccgg aacgttcacg acgggcgccg gtgcgggacg
     4981 agaggctctc gcccatgatg ctgaaaccgg cgacgatcga cctgcccgct gcagtggact
     5041 tcatggcgac ccacgctcgt ccgctcgacc ggcaccgcct ccggctgctc ctcggcacgg
     5101 gcgccccgga gcctgtgctc gccgcaaccg aggcgtaccg caacgccgac ggcggctacg
     5161 gctggggact ggaaccggac ctgcgatccg gcacgagcca accgggagcc gcgctgcacg
     5221 cgttcgaggc gttcgaggac atcgctccca tgacgacgac acgcgccggg gcgctgtgcg
     5281 actggctgga gtcggtgtcg ctgccagccg gcggccttcc gttcgcgctg ccggtgaacg
     5341 atccggcagg caccgcccgc tggtgggcgc aggccgacgc aggggcgtcg tcgctgcaga
     5401 tcaccgccgt cgtcacggct gcggccctgc gcgtggcacg gcacgacccg gtcgtcgctg
     5461 cccacccgtg gaccgcccgt gccacccggt actgcctcga tgccgtgcgg gccgtggagg
     5521 agcgcccgca cgcgctcgtc ctggccttcg cgctgcagtt cctcgacgcc gcgcacgaca
     5581 gcgagcccga ggcggctgcg ctcctcgatc ggctcgccac gcacctcccg gacgacggca
     5641 tccttcccgt cgacggcggc acggagagcg aggtgatccg cccgctggac atcgcaccgg
     5701 cccccgatcg tccggtgcgg tcgctcttcg ctcccgacgt ggtcgtcgcc gacctgcacc
     5761 ggctcggcgg gacgcagcag gacgacggcg gctggcgggt ggagttcgcg gagttctccc
     5821 cggcaggcgc gctcgactgg cgaggtcacg ccacggtgcg ggcggtggcg gtcctgcgcg
     5881 ccaacggcgt gatctgagcc gtgcccgtgc ggtcagggga ccagcaacag cgcggtgggt
     5941 accgccacca gcccggcggc agccaaccgc accagcacgc ggacagcacc cggcaggggc
     6001 ggtggcggcg tggtgatccg gtccagccgg tggtccagcc cgtcggtgaa cgagctcaac
     6061 gcggcggccg gtgctgcacc gcccattgtc cgcagcgccc cggtcagctg gttcgcggtg
     6121 acctccgccg acgccacgtc gtcggccctc atctcgatca acgcggccac cgccagctgg
     6181 gcgcagacca tgccacgcac gaacggcgcc gtcgcccccc aggccacgaa cggcagcacc
     6241 acgaggtcgt gacgttcccg caggtgggcc tgttcatggg cgagcacggc ccggacggca
     6301 gccggctcga gggcatcgag gacgccggcg gagagcacga gccgcgaccg caatccaggg
     6361 aggcagtacg ccacggggat cgggtggtcg agcacccggg cgccgggtac ggctggccac
     6421 ggcgtaccca gcaggtcgag caggtcccgg tggcgacggc ggacgcgcag cgtacgcagg
     6481 gtgaccactg ccagcacact gaccagccgc agggcgagcg ccagcgccgc cagcaacgcg
     6541 gcgaagtggg tgaccgtgag cgccggggga acctcgccga cggccaacgc gccgaccgcc
     6601 acggaggcgg cgaccgacgg cgagggtccc agcggcgaca ggccgtacat cacccccgag
     6661 ccgagcagcg cgaccccgcc cgcgagcccg accgcctgcc acacgagcag ggccccgaca
     6721 gggtcgcggg caggccagtg cgcggcggcg agcgcccggc tgaccggctc ggcaaggaga
     6781 acgccgagca cgagcaggcc gaacgcagtg agcgtcatcg gacgtaggtt ggttcagccg
     6841 agcagctcgc gcaatgtggc gcgctcgtcg gccgggatcg agccgaggaa ccgggccaac
     6901 gccgcgcccc ggtcgggtgc cgcgtccagc gcgtcgcgca tgagctcggc gacgtggtcc
     6961 tcgcggctgg ccaccgagcg gtagcggtgg gcccggccct cgcgctcccg cgtgacgagg
     7021 cccttgcgct ccagccgccc cagcactgtg agcacggtgg tggtggccag gtcacggcta
     7081 cccaaggcgt cctgcacgtc gcgggccgtg acaggcccgg cggccgacca cagggtctcc
     7141 attacggcac gttccagctc acccagcgtc ggcacccacc tattctactt caggtagaac
     7201 gcttctggta catcggtgag tggatctgcg cttagtcacc tacctgcagg aagtgcgacc
     7261 acgctccgga accggcgtac ctccgtccgg atctccgttg agatcttcgg ggccggcggc
     7321 cagccgtccg gtcggcttct cggtgtcctc gctggtccag catgcttcga aggccgcatc
     7381 cagagcgggt cgtcgacgag cgggtcggtg cgcctgagcg cgagaggggc cgcggtgagc
     7441 ctcaccgggc gctcacacca cggcatagtg acgtccatgg cggcggaccc gggcgcaatc
     7501 cgaatgggca ccgcacgagg ccgatgggtg ctcctgacca ccgtgctcgg gtccggcctc
     7561 gtgatgatcg acagcacggt ggtgaacgtc gcgctcgcgc acatcggcac cgagttcggc
     7621 gcgggtttca tcgcgctgca gtggacggtc aacgcctaca cgctcaccct cgcctcgctg
     7681 atcctcctgg gtgggtcgct cggtgaccac ttcgggcgcc gccgcatctt cctgatcggc
     7741 gtcgtgtggt tcgcgctggc gtcactgatg tgcggtctcg cccccaatat cgagaccctc
     7801 atcgcggcca gggcactgca gggcgtcggc ggcgcgctgc tcaccccggg cagcctggca
     7861 ctgatctcgg cgtcgttcca cggaccggac cgggcggctg ccgtcggtgc ctggtcgggc
     7921 ctgggcggga tcgccggcgc cgtcgggccc ttcctcgggg gttggctggt ggagtggaac
     7981 tggcgggcgg tgttcctcat caacctgccg ttggccgtgc tgatcgtcgc ggtggcggtc
     8041 cggcacgtgc cggagagccg cgacaccgag tccgccgccg ggctcgacat cgccggcacg
     8101 gtgctcgccg tgctcggcct cgccggcctg acgtactcgc tcaccgggct cagcgcgcgt
     8161 ggtgcggctc cggacctggt cgccggcctg atcgtcgggg tcgtggcact ggtcgcgttc
     8221 gtcctgtggg agcggcgctc accgcatccg ctggtgccgc ccgaactgtt caggaacacg
     8281 acgttcagcg ccaccaacgc cgtgacgctc ctgatctacg gcgccctcgg cgtggtcttc
     8341 ctgctgctgg tgctgcaact gcagatcgtc gtggggttca gtccactcgc cgccggcacc
     8401 tcgctgctgc ccgtcacact gatcatgctc gcgttctccg cgcgcgccgg ggcgctcgcc
     8461 ggacggatcg ggccgcggtg gccactgacg ctgggcccgc tgatcagcgc ggcagggctg
     8521 ctgctcatgc tccggatcgg acccggcgcc tcgtggctca ccgacgtgct gcccgccgtg
     8581 ctcgtcttcg gcgcgggtct ggcgctcacc gtcgccccgc tgaccgccac cgtgctcgac
     8641 agcgcccccg acaggtacgc cggagcggca tcgggggtga acaacgcggt cgcccgagca
     8701 gcggggctgc tcgcggtggc cgtgattccc ggcatcgccg gcatccgggg cgacgactac
     8761 accgaccccg cctcgttcgg caccgggttc cggatcgcca tggtgatcgg agcgggactg
     8821 ctggtcgcgg cgtcgctgct ggcgttcgtg gtggtccggc gcccgctccg ggcagcggag
     8881 cccgatgcag cggagcccga tgcagcggac accggcgcgc cggagcgggt gcggatcgag
     8941 gagtgcatgc attgcccggt gaccggaccg caggtgcttc ccccaccgga ccgggcgctc
     9001 ggcaaccgct gaaccgacgc ctcgcgtccc cgaccacgct ccaccgccga ggcgcggcgg
     9061 cttaccgcag gttcagcgcc tccgcgagct cgcgcaacgc gggcaccggg tcgtccccac
     9121 ccggcagcac ctgcgcacag acgtggtcgg cgcccgcgtc gaggtgctcg gtgagccgtg
     9181 cggcgacggc ggcggcgtca ccgtgggcga cgagcgcgtc gatcagccgg tcgctcccac
     9241 cgtcggccag gtcctcctcg ctccacccca ggcgcagcag gttggacgtg tagttgcgca
     9301 ggccgaggta gggcttcgca acgacgggac gcccgatcgc ccgggcgcgc tccgggtcgg
     9361 tgtcgagcac caccttgtgc tccggcgcga gcagcacgcc ctcacccaag agctcccgcg
     9421 cctcgcgggt gtgctccggc gtggtcaggt aggggtgcgc gcccgccgtg cgctcggcgg
     9481 ccagcttcag caccttcggc ccgagcgcgg cgagcacgcg cccgtccacc ggcaccttcg
     9541 cggcgtccag ccgatccagg tactcgacga tggtggcgta gggacgccgg tagtcgctgg
     9601 tcgcctcggg gtggccgatg cccacgccga gcaggaaccg gccggggtgc gcggcctcga
     9661 tccggtggta ggactcggca gcttcctccg caggtgtcgt ccacatgttg acgatgccgg
     9721 tggccaccgc gatccgctcc gtggcgtcga gcagcgactc ggcgagccgc aggtccccgg
     9781 gaggcgagcc gccgatccag atggcgccgt agcccagcgc ctccacctcc gccgccagct
     9841 ctggggtgag cccacccacg tgccgccaga cgccgtaccg acccagttct accgtcatga
     9901 cggcggcaac cccgcagcgc cccggcgcat tccggcaagt gtcggtgcgg gcacataggg
     9961 tctttccgtg agccgagagc cgaccacccg ggcagccccg ctgcggatgc gtccgcgggt
    10021 ggtggtgccg cccgatccac ggcagccgcg cgaggccccc gccggcacgg aggcggtggt
    10081 cgtcggaggt ggggtggcgg ggatcagcgc cgccgtcgtg ctcgccgagc gcggcgtcgc
    10141 ggtcacgctg ctggaggccg cgcccaccct cggcggccgg ctcggcgcct ggccacacac
    10201 gttgcccgat ggcaccgagc aggtggtcga gcacggcttc cacgcgttct tccgccacta
    10261 ctacacgtgg cgcgcggtgc tccgccggat cgacccggcg ttgagcttcc tgcgcccggt
    10321 cgacggctac ccggtgatct cacgacagtg gccgcccgag gacctcaccg ggttgcccgc
    10381 ggcgccgccg ctcaacctgc tggcgctgtt cgcccgctcc ccgagcctgg gcctgcgcga
    10441 tctcctcggc tcccggcagt cgctggccgc cgagctgctc gcctacgacc gcgagcgcac
    10501 caccgcggcg ttcgacacag tgcctgctgc ggagttcctc gccgcgctgg gcatgtccga
    10561 ccgcgccagg gcgatgctgt tcgaggcgtt cgcacggtcg ttcttcgccg agcccggagc
    10621 gctgtccgct gccgagctca tcgcgatgtt ccactactac ttcctgggca accccgaggg
    10681 catcggcttc gacgcacccg ccaccgacta cctcacctgc atctgggagc cgttccgggc
    10741 cttcctcgtg cggcgcggcg ccgagatccg cacctccacc gccgcacggg cgatcctccc
    10801 tgccgacggg caccgctggc gcgtcgagct ggccgacgca gctccgctgg agacccgtca
    10861 tctggtgctc gcgaccgagc ccggcgcctt gcgaagcctg ctcgccgcct cgccggccgc
    10921 cgccgccgtc gcaccgcggc tggcggcgaa gacggccgcg ctcaccgtgg ctccaccgtt
    10981 cgcggtcacc cggctgtggc tcgatcgcga tgtcgcgcca gaacgttcga cgttcagcgc
    11041 cgtcagccgg gagcccacgc tcgactccgt caccctctac tcccgcctgg agcggccgtc
    11101 cgccgcgtgg gcggcgcgca ccggcggagc ggtggtggag ttgcactcct acgcctgcgc
    11161 cgcacccgac gccgacacgg cgacggcgcg gatgcgcgcc gagctggcag cgctctggcc
    11221 ggagacggcg gccgcaggcc tggtgcacct gcagcagcgg ctggaggcca cggcccccac
    11281 cttcccgccc ggcggcgccg gcaccaggcc cacggtcagc ggcgaggcca ggggaatccg
    11341 ggtagccggc gacttcgtgg agacgccgtt cctcaccggc ctcatggagc gtgcatcggc
    11401 gtcgggggtg ctggcggcca acgacgtgct cgccgaggtg ggcgccggcc cggaggagat
    11461 cttgggtggg ccgcaacgcg ggttgctcgc cgggctgctt cctgcgcgag ccacaccgcg
    11521 ctggtcaaca ccgccctcgt cgataccggg tcgggtgcgc gtggagcccg cagcagcgcc
    11581 ctcggcggcg cggcgcaggt gaggatcgcc ctcgatccgg ggccacgcga cggcccggtc
    11641 cgtatccgcc ggctcgccga tgacggcgcc ccgctcgggt ccgaggagac cctgaccgac
    11701 ctcgcgcgtg tcgccgagct ggagcgcgag cacgggccac gatgggtgtt cgcccgtgcg
    11761 cacacctacc ggcggctgct ggctgctggc gtggaagtgg cgcgtgctca cgacgtcgag
    11821 ctggtggagg gcctgctcgt gggtgctgcc ggccactacg gccagccgca cggcctggcc
    11881 gccgccaccg cccggctgca cggccgcact cccgaccccg acccgcccgc tggcgacgac
    11941 ggtccaccgg cattgttcga gacggggcct gccacagacg agctgaccga cgtcgtggcc
    12001 gtgcacgccg cccagcagcg cgcgctcgcc gcggccgggc caggaatgcg gctcctcgcg
    12061 gccgccgagt ccgccggggt gctcgtggcc gaggagatga cgtgttacgg cctgccgtgg
    12121 cgcgcggaga tccacgatca gctgctcacc gagctgctcg gcccgcgtcc tgtgctcggg
    12181 ggcaggccgc cgaagctggc cgcgctcgcc gaggacgtca cgacggcctt cgacgggcgg
    12241 ccgctcaacc ccgactcccc cgccgaggtg ctgcgcgcct tcgcccgggc cgggcacgag
    12301 ctggagacca cacgctcgtg ggagctgcgc cggatcgacc atccggccgt cgagccgctg
    12361 ctggcgtaca aggagctggc ccggctgcac tccgcccacg gatggtcgtg gctgcgcacg
    12421 tgggtcgacg gcggccggtt ccggccggag tacgtggtcg gcggcgtggt gtcgggccgg
    12481 tgggcatccc gcggcggggg cgccctgcag atcccccgcg ccgtgcgcgc cgccgtccgg
    12541 gcggaccccg gatggcgcct ggtcgtggcc gacgccgcgc agctggagcc acgggtgctt
    12601 gcggcgctct cgggcgatca cgcgctcgcc cccggcgagg acgacctgta caccgccctc
    12661 gccgctcgcg agttcgaggg cgaccgggcc agggccaagc tcgcgctgct gtccgcgatg
    12721 tacggccaga cctccggtgg tgccggagtg ctgctggcca cgttgcgcag gcggttcccc
    12781 gcggcgatgg cctacgtgga ggacgccgcg agggcaggtg aggagggcag gctcgtgcac
    12841 tcacac
//
LOCUS       DASRSC010000235        11507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4019706,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000235 DASRSC010000000
VERSION     DASRSC010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11507
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4019706"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(330..1673)
                     /locus_tag="VFE39_11460"
     CDS             complement(330..1673)
                     /locus_tag="VFE39_11460"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HET6259258.1"
                     /translation="MDRQQEFVLRTLEERDIRFVRLWFTDVLGYLKSVAVAPAELEGA
                     FAEGIGFDGSAIEGFARVYESDMVARPDPSTFQVLPWESSSGEHYSARMFCDIAMPDG
                     SPSWADPRHVLRRALSRAGEAGFTCYVHPEIEFYLLRDLPNDGSPPVPADNGGYFDQA
                     SHDVAPHFRRNAIETLESMGISVEFSHHEGGPGQQEIDLRYADALTMADNIMTFRYVV
                     KEVAITQGVRASFMPKPFSAHPGSAMHTHFSLFEGDRNAFHDPNDPFELSETGKAFVA
                     GVLRHAREISAVTNQWVNSYKRLITGGEAPTAVSWGHANRSALARVPMYSPGKSSTRR
                     VEVRTLDTACNPYLAFAVILGAGLTGVQKGYELPPPTEDDVWSLTETERRAMGYESLP
                     QNLTEALAAMEHSELVAEILGEHVFDFFLRNKRAEWDGYRRQVTPYELKTYLPVL"
     gene            complement(1683..4619)
                     /locus_tag="VFE39_11465"
     CDS             complement(1683..4619)
                     /locus_tag="VFE39_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HET6259259.1"
                     /translation="MALEFRVLGTLEVTSDGGRLSLPSTRHQRVVAALLLAPNSVVPL
                     SKLVEAVWSDEPPATAVKQVQNCVSVLRERLGGQVILTDGPGYRIAVGEDQLDLLRFH
                     RGLSDARRLAAEGRLADAVREARAGLGLWRGSVLDGLCISVLTNRVTLLNEQRLGAIE
                     QCVDWQLELGEHRDVAAELMELVAEHPLREKLHAQLMLALNRGGRQADALKVFHDLRT
                     RLVDELGIDPGSEVLQLHERILAGEPQVPRTVPRPPAAEAGAGTEAPVAGLRDDRLDL
                     AVRELASAISRQWTVEVEMRSLHRPEPVPLRWSSTGRQVAAVASAVLGDGPDGARPDR
                     LSLSGGLGDVVGQFRQLPTRQLVVLGEPGAGKSVLAILLTLGLLAEPLPAEPIPVLLP
                     LASWNPHREHLHRWLARRLVEEYPALANRSAYGRDAATRLILEGRVVPVLDGLDETPP
                     ALHAAAIDALDQAVAGGRPLVVTCRSTEYELAVNQAGSMLARAAVVEIEPVEVDDAIA
                     FLTARRRLGETRWQPVVDHLRRNPAGVLAQALRTPLMVDLARTAYAHPCSNPADLCDT
                     TRFPDRGTIEGHLLDAYLPAVYRQTPAPPAQGDRPTAARSYDPDQAERWLTFLAQHTH
                     RLPAGDLAWWQLDKAVPRLVSSVYLGLPAAVLFAVAGWLAGGPVIGMIYGLSFAAAGC
                     VSHAFGRRSGPLRVEFRIRGTTMRFLRRCAVGVVIAVSLGLGWSLSVGVVILLAVVFG
                     LAVGAHVWVGTPVDARLVSSPASVLRNDRVAAMSFALSLLVSLGLFYGMAFAFTRETR
                     FIGVLNGRFDLALVLAGGLASALLGRFLMRNMGSVAYGLAGAVVGGLVFPRATNPTTA
                     IAVGIVFGLAVGLTVCLARAWGAAVLNRVWLASRGHTPLRLMRFLDDAHRRGVLRQVG
                     AVYQFRHLRLEERLTVRASQDHERSPRRRAGVREGAGPSVRTPDRVPDVAATEQRRNM
                     SVTRRS"
     gene            complement(4938..6497)
                     /locus_tag="VFE39_11470"
     CDS             complement(4938..6497)
                     /locus_tag="VFE39_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6259260.1"
                     /translation="MRWTSFAAALCSAVLLLVACTQATTGSAAAVAPDRAQLARFYDQ
                     KLSWGPCADFATTAGDRQNFADPKFDCARLEVPLDYNAPDGQTAQIAVLRQRATGARI
                     GSLLVNPGGPGASGASLAASLSGRIAQTPLAQRFDLVGFDPRGVGASTPTIDCLNDAE
                     WEVERADLDVDPSPAGVAQTEAENRQYANRCSQRSGGVPVLANAGTRDVVKDVDILRA
                     ALGDEKLTYLGYSYGTRIGSAYAEAFPQNVRALVLDGALDPTETTIDRTVKQNAGFQQ
                     AFDAYAADCARQPNCPLGTNPAKATAAFQALTRPLIDKPARAEGGSRTLSYPDAVTGT
                     IQALYLSQLWPALSRGLSGLATGDGTILLRLADIYNDRGPDGHYGNGIEAFVAISCVD
                     EQRITDRAQQAELIRRSNEAAPFRDDGRGIVPALDPCAFWPAPPTSQPHVPQVEGLPP
                     TLVVSTTGDPATPYQAGVDLAKALKGSLLTFEGTQHTVALQGVPCVDDAVAKYLIDAT
                     LPPPDTVCRAS"
     gene            6705..7586
                     /gene="panB"
                     /locus_tag="VFE39_11475"
     CDS             6705..7586
                     /gene="panB"
                     /locus_tag="VFE39_11475"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HET6259261.1"
                     /translation="MSHTPISVTGGEVQPPYRSGPASGGQSRPKRTRIHHLREFKERG
                     ERWPMLTAYDMYSAEIFDAAGMPVLLVGDSAANNVYGYENTLPVTVDELLPLVKAVVR
                     GTSSALVVADLPFGSYQISPEQALTTSFRFMKEGGAHAVKLEGGARFVPQVEALAGSG
                     IPVMAHLGFTPQSEHALGGFRIQGRGDAGERLVEDALALQAAGAFSVVLEMVPADVAK
                     RVTAELVIPTIGIGAGPDTDAQVLVWSDMAGMNRGRKPRFVKQYADLGGVLLDAAKRF
                     GDEVRGGMYPDAEHSYS"
     gene            complement(7868..8989)
                     /locus_tag="VFE39_11480"
     CDS             complement(7868..8989)
                     /locus_tag="VFE39_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HET6259262.1"
                     /translation="MEPRAVQEDRAFRYGIEHECALLHSDGGFADYTDTGFDELQAVI
                     DALPEDPDDYPELRVGDQGIKRKRWYVEGYERFDTHGRLVRCDPKGIEIRTRIHGSVD
                     AAVGALRADVARLDGALAPTRLRTTVIGFNPLRSEYRIEPPLNEWERQHRLGSPEERT
                     AHLHMSTYGPDLNLSCATLGTAALMDIGRKLTHYSPWLVPLSFSSPFRDGAPWGGLSA
                     RTAVRTGLRPAVLVFLGERDEQVDADPSLTQEARIPAEVGRIEFKAFDACPDPVLYGE
                     LLSWLTGLVLDETLPGRRTTPDAALHQHVALAGLGDDDVHACTGALLDAAAAALAGRD
                     DDLGRLESLRGRWARRECPAVTMLARYEDGEPVAGLRHP"
     gene            9069..9632
                     /locus_tag="VFE39_11485"
     CDS             9069..9632
                     /locus_tag="VFE39_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HET6259263.1"
                     /translation="MIGEDPLAVLRDRRSRAALASPAPPRGELQEILDAAWSVPDHGR
                     LRPWRFIVIEEAARDLLGDAFAAAHAEREPTADPAELHRTRKKPLRAPLIVVVVATPR
                     PHPKVPVWEQRATAACVAYSVVLAAHVRGLGAMWRTGWYGEAPKVRAHLGLVDGEEVT
                     GWVYLGTPAGPEPPPRPPADPPVTWFS"
     gene            complement(9653..11092)
                     /locus_tag="VFE39_11490"
     CDS             complement(9653..11092)
                     /locus_tag="VFE39_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HET6259264.1"
                     /translation="MHLPPTRAPRGAPQFAAVRAEFALPDTFPAEVLAEAATAASRPP
                     TAGPERVDATDLELVTIDPRGSKDLDQALGVSRRGGGFRVHYAIADLGVMVSPGGALD
                     AEARRRGQTIYLPDGPVPLHPPTLSEDAASLLPDGSRGAVLWTIDLDDAGEPVTVDVR
                     RAVVRSRARLDYAGVQADAEAGRLHPALVALPELGLLRRHQAVRRGVIELELPEQEVV
                     PDGLGGWTVQVRRRLDVEVWNAEISLLTGMAAARIMIDAGVGLLRTLPAVEPEALEGF
                     RRAARELGFTWPPGSTAAEILADLPGDAPAALALRRAATILLRGAGYAAFDRDAGVPV
                     PDDAGHGGIGAPYAHVTAPLRRLADRFGTELCLAVTAGLEPPSWLREALPALPEVMTV
                     SDTLAGKVDRACVDRTGAALLAGRVGEEFDGVALRPNGPEGELGEVYLPDPPVLARCA
                     GPLTAGASVRVRLAVADPTTGRIEFAAVS"
     gene            complement(11135..>11507)
                     /locus_tag="VFE39_11495"
     CDS             complement(11135..>11507)
                     /locus_tag="VFE39_11495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HET6259265.1"
                     /translation="WSAQVRNVQGQEIPATDIPWIRAREMWVHAVDLDVGASFADLPA
                     PMLAELLTEVATTMGAKADCPSLRLVAPDGTWTVGDAENAITVSGPAPELLEWLLGRG
                     KGRALRSSTEGRKLPVLPRWL"
BASE COUNT         1705 a         4118 c         4112 g         1572 t
ORIGIN      
        1 gcggccccca cgacctcccc ggcccggtac accgtcaaca aagccgtcgc gggctcaccg
       61 gtgcggcaca gagcgtcgat cacggtcagc tcgaccgtgt accggatcgg gtcggcgccg
      121 agcagcggga gggccagccg ggcgaactcc tcgacgtcat cgtgcagaag gatctccacg
      181 cctcatactc tccaccgccg ttgccgcagc tgcgatcgct tatcgaccat gatcagcagt
      241 tcgacatgcc gagccgcgcc atgctcgacc atgggccccg agccgctgat cacgggtagc
      301 cgggtgacag agggtggaga aggccggcgc tagaggaccg gcagatacgt cttcagttcg
      361 tacggcgtga cctggcggcg gtacccgtcc cactccgccc gcttgttgcg caggaagaag
      421 tcgaacacgt gctcgccgag gatctccgcg accagctccg agtgctccat ggccgccagc
      481 gcctcggtca ggttctgcgg cagcgactcg taccccatcg cgcggcgctc ggtctcggtg
      541 agcgaccaga cgtcgtcctc ggtgggcggc ggcagctcgt agcccttctg cacgccggtg
      601 agcccggcgc cgaggatcac cgcgaacgcc aggtaggggt tgcacgcggt gtcgagcgtg
      661 cggacctcca cccgtcgagt ggacgacttg cccggggagt acatcgggac cctcgccagc
      721 gccgagcggt tggcgtgtcc ccagctgacg gctgtgggcg cctcgccacc ggtgatcagg
      781 cgcttgtagg agttgaccca ctggttggtg accgcgctga tctcccgtgc gtgccgcagg
      841 accccggcca cgaacgcctt gccggtctcc gagagctcga acgggtcgtt cgggtcgtgg
      901 aaggcgttgc ggtcgccctc gaacaaggag aagtgcgtgt gcatcgccga gccggggtgg
      961 gccgagaacg gcttgggcat gaacgaggcg cgcacgccct gggtgatcgc gacctccttc
     1021 acgacgtacc ggaacgtcat gatgttgtcg gccatcgtga gcgcgtcggc gtagcgcagg
     1081 tcgatctcct gctggcccgg gccgccctcg tggtggctga actcgacgga gatacccatc
     1141 gactcgagcg tctcgatcgc gttgcgccgg aaatgcggtg cgacgtcgtg gctggcctgg
     1201 tcgaagtacc cgccgttgtc ggcgggaacc ggcgggctgc cgtcgttggg caggtcacgg
     1261 agcagataga actcgatctc ggggtggacg tagcaggtga acccggcctc gcccgccctg
     1321 ctcaaggcgc gccgcagcac gtgccggggg tcggcccacg acggtgagcc gtcgggcatc
     1381 gcgatgtcgc agaacatccg ggccgagtag tgctcacccg acgagctctc ccacggcagc
     1441 acctggaagg tcgagggatc gggccgcgcg accatgtccg actcgtacac ccgcgcgaag
     1501 ccctcgatcg cggacccgtc gaagccgatt ccctccgcga acgcgccctc cagctcggcc
     1561 ggggcgacgg ccaccgactt gagatagccg agcacatcgg tgaaccagag gcggacgaag
     1621 cggatgtcgc gttcctcgag tgtgcggagc acgaactcct gctggcggtc catgcgccga
     1681 gcctaggaac gtcgcgttac agacatgttt cgtcgctgct cagtagctgc gacatcgggc
     1741 acacgatcag gagtccgcac cgacggtccc gcgccttccc gcacgcccgc cctacgccgg
     1801 gggctccgct cgtggtcctg cgatgccctc actgtgagac gttcctccag acggaggtgg
     1861 cggaactggt agacagctcc cacctgccgc agcacaccac gacggtgggc gtcgtcgagg
     1921 aaacgcatga gccgaagcgg ggtgtggccc cgtgacgcca gccagacgcg gttgaggaca
     1981 gcggcccccc acgcccgggc caggcacacc gtgagtccga cggcgagccc gaacacgatg
     2041 ccgaccgcga tggcggtcgt cgggttcgtc gcgcgcggga agaccagacc gccgaccacc
     2101 gcaccggcga gtccgtaggc aacacttccc atgttcctca tcaggaaacg gccgagcagt
     2161 gctgacgcca gcccaccggc gagcaccagg gcgagatcga agcgaccgtt caggaccccg
     2221 atgaaccggg tctcacgggt gaaggcgaag gccatcccgt agaagaggcc cagtgacacc
     2281 agcaaggaca gcgcgaacga catggcagcg acccggtcgt tcctcagcac actggccggg
     2341 ctggacacca gcctcgcgtc gaccggagtg ccgacccaga cgtgggctcc gacggcgagg
     2401 ccgaagacca ccgcgagcag gatgaccact ccgaccgaca acgaccagcc gagccccagg
     2461 ctcaccgcga tcaccacgcc gaccgcgcac ctgcgcagga acctcatcgt cgtgccccgg
     2521 atccggaact cgacgcgcag cggccccgat cgtcgcccga aggcgtgtga cacgcagccg
     2581 gccgcggcga acgagagacc gtagatcatc ccgatcaccg gaccaccggc gagccagccg
     2641 gcgacggcga acagcacggc ggcgggcagg ccgaggtaca cgctgctgac cagtcgcgga
     2701 actgccttgt cgagctgcca ccaggcaaga tccccggcgg gcagccgatg cgtgtgctgg
     2761 gcgaggaagg tgagccaccg ctcggcctgg tccggatcgt aactgcgcgc agccgtcgga
     2821 cggtcgccct gtgcgggcgg tgccggcgtc tgccggtaga cggcgggcag ataggcgtcc
     2881 aggaggtggc cttcgatcgt gccccggtcg gggaaccggg tggtgtcgca gagatcagca
     2941 gggttcgagc agggatgcgc gtaggcggtt ctggccaggt cgaccatcaa cggggtacgg
     3001 agtgcctgcg ccaacacacc ggcgggattc cggcgcaggt gatccacgac aggctgccag
     3061 cgggtctcac cgagccgtct gcgtgcggtg aggaaggcga tggcgtcatc gacctcgacc
     3121 ggttcgatct cgacgacggc ggctctggcc agcatcgatc ccgcctggtt caccgcgagc
     3181 tcgtactcgg tgctgcggca ggtcaccacg agcgggcgtc cacccgccac cgcctggtcg
     3241 agggcgtcga tcgccgcggc gtgcaacgcc ggtggcgttt cgtcgagacc gtcaagcacc
     3301 ggaacgaccc tgccctcgag aatcagccgg gttgcggcgt cccggccgta ggcggatctg
     3361 ttggccaggg ccgggtactc ctcgacgagt ctgcgggcca gccaccggtg cagatgttcg
     3421 cggtgggggt tccacgaggc caagggcagc agcaccggta tcggctcagc gggcagtggc
     3481 tcggcgagca gccccagcgt gagcaggatc gccagcacgg acttaccggc accgggttct
     3541 cccagcacca ccagctggcg cgtggggagc tgccggaact gcccgaccac gtcgccgaga
     3601 ccaccgctca ggctcaggcg atcgggccgg gcgccgtccg gtccgtcacc cagcacggcg
     3661 gaggccaccg cggccacctg ccgtccggtg ctcgaccacc tgagcggaac cggttcgggc
     3721 cggtgcagcg atcgcatctc gacctcgacc gtccactgcc gggagatcgc cgacgcaagc
     3781 tcccggaccg ccaggtcgag acggtcgtcg cggaggccgg cgacgggcgc ctctgtgcca
     3841 gcccctgcct cggccgccgg tggacgcggc acggtccggg gtacttgcgg ctcgccggcg
     3901 aggatgcgtt cgtgcagctg gagcacttcg ctgccgggat cgatgccgag ctcgtcgacg
     3961 agccgggtgc gcaggtcatg gaagaccttc agcgcgtcgg cctgccgtcc gccccggttc
     4021 aacgccagca tcagctgcgc gtgcagcttc tcgcgcagcg ggtgctcggc gaccagctcc
     4081 atcagctcag cggcgacgtc gcggtgctcc cccagttcga gctgccagtc gacgcactgc
     4141 tcaatggcgc cgagccgctg ctcgttcagc agagtgacgc ggttggtcag cacggagatg
     4201 cacaggccgt cgagcacgga gccacgccac agccccaacc ccgcgcgggc ctcccggacg
     4261 gcgtccgcga gcctgccttc tgcagcgagc ctgcgggcgt cggacaggcc gcggtggaac
     4321 cgcagcagat ccagctggtc ctcgccgacc gcgatccggt aaccgggccc gtcggtgagg
     4381 atgacctgac caccgaggcg ctcccgtagc accgatacac agttctgcac ctgcttgacg
     4441 gcggtcgccg gtggctcgtc actccagacc gcctcgacca gcttcgagag cggcaccacc
     4501 gagttgggcg ccagtagcaa ggctgcgacg acccgttgat gccgggtgct cggcagactc
     4561 agcctgcccc cgtcgctggt cacttcgagc gttccgagca cgcggaactc caacgccatg
     4621 cttctccccc ggcacgtcct gcgggggatg ctacccgcag tagctggccg aacactgctg
     4681 gcgccggatt tcggcggaag gacggcgtag ggacaaggtg atgacgagga ctgtcacgtt
     4741 ctgtccgtcc cgttcccacg aatggggcat cggtgcggcg gatcggtgat tgccagtcgt
     4801 ggggggttgt caatcaccgt tccgcacgcg ccgcgcgttc ccacgcgagt acgtcacggc
     4861 ccaggcgggc ctggtgctgc tccgccggcg gcctcctgac gaggatgccg acctgcgcgg
     4921 tcgcaggagt cgcgggatca gctcgcgcgg cacaccgtgt cgggaggggg cagcgtggcg
     4981 tcgatcaggt acttcgcgac ggcgtcgtcc acgcacggaa caccctgcag ggccaccgtg
     5041 tgctgcgtgc cctcgaaggt caacaggctg cccttcagcg ccttcgccag gtccaccccc
     5101 gcctggtagg gagtggccgg gtccccggtc gtcgagacga cgagcgtcgg cggcagtccc
     5161 tccacctgcg gcacgtgcgg ctggctggtc ggcggggccg gccagaacgc gcacgggtcg
     5221 agcgccggga cgatgccacg cccgtcatcg cggaacgggg cagcctcgtt ggaccgacgg
     5281 atcagctccg cctgctgggc gcgatcggtg atccgctgct cgtcaacgca gctgatggcg
     5341 acgaacgcct cgatgccgtt gccgtagtgg ccgtccgggc cgcggtcgtt gtagatgtcg
     5401 gccagtcgca acaggatcgt gccgtcaccc gttgcgagac cggacaggcc gcgggacagc
     5461 gccggccaca gctgggacag gtagagcgcc tggatggtgc ccgttaccgc gtccgggtag
     5521 gacagcgtcc ggctgccgcc ctcggcccgg gcgggcttgt cgatcaaggg gcgcgtgaga
     5581 gcctggaacg cggctgtggc cttcgcgggg ttggtgccca gcgggcagtt cggctggcgg
     5641 gcgcagtccg cggcgtaggc gtcgaaggcc tgctggaagc cggcgttctg cttcacggtg
     5701 cggtcgatcg tggtctcggt tgggtccagg gctccgtcga gcaccagggc acggacgttc
     5761 tggggaaacg cctctgcgta ggccgagccg atccgggtgc cgtacgagta gccgaggtag
     5821 gtcagcttct cgtcgccgag cgccgcacgc aggatgtcga cgtccttgac gacgtcgcgg
     5881 gtgcccgcgt tcgccagcac cggcaccccg ccggagcgct ggctgcaccg attcgcgtac
     5941 tgccggttct cggcttcggt ctgcgcgacg ccggccggcg acgggtcgac gtccaggtcg
     6001 gcccgctcga cctcccactc ggcgtcgttc aggcagtcga tcgtcggggt gctcgccccg
     6061 acaccgcgcg ggtcgaaccc gacgaggtcg aagcgctggg ccagcggagt ctgcgcgatc
     6121 cgtcccgaca gggacgccgc gaggctcgcc cccgacgcac cgggaccgcc cggattgacc
     6181 agcaacgacc cgatccgtgc gccggtcgcc cgctgcctca ggaccgcgat ctgtgcggtc
     6241 tggccgtcag gggcgttgta gtcgagcggc acctcgaggc gcgcgcagtc gaacttcggg
     6301 tcggcaaagt tctgacggtc gccggcggtg gtggcgaagt ccgcgcaggg gccccaggac
     6361 agcttctggt cgtagaagcg ggccagctgc gctcgatcgg gagccaccgc agcagccgag
     6421 ccggtcgtcg cctgggtgca ggcaacgagc agcagaacgg cggagcagag agcggcggcg
     6481 aatgaggtcc atctcatgcg ggctggcacc gcgccagcat gccagcgccg ggcggttgtg
     6541 catctcctgc cgcgctcgcc gcctcacacg tccggtgagg ggtcctcgtg tgtgaacctc
     6601 gtcccacaga cggtgcagtg acgctgcgcg gtgtcacgct ttctgtgtca gcacctgacg
     6661 acccggggac ccgcgcaacg ggcctcgagg gaggacggac aacgatgtct cacacaccga
     6721 tcagcgtgac cggcggcgag gtccagccgc cgtaccgctc cggacccgcg tccggcggcc
     6781 agtcaaggcc gaagcggaca cggatccacc acctgcgcga gttcaaggag cgcggcgagc
     6841 ggtggccgat gctcaccgcc tacgacatgt actccgccga gatcttcgac gcggccggca
     6901 tgccggtgct gctcgtcggg gactcggccg ccaacaacgt ctacggctac gagaacaccc
     6961 tgccggtgac ggtcgacgag ctgctaccgc tggtcaaggc cgtggtgcgg ggcaccagct
     7021 cagcgctcgt cgtggccgac ctgccgttcg gcagctacca gatcagcccg gagcaggcgc
     7081 tcaccacctc cttccgcttc atgaaggagg gcggcgcgca cgccgtcaag ctcgagggcg
     7141 gggcgcgctt cgtcccccag gtcgaggcgc tcgcggggtc gggcatcccg gtcatggccc
     7201 acctcgggtt cacgccccag agcgagcacg ccctgggcgg attccggatc cagggccgcg
     7261 gggacgccgg cgagcggctg gtcgaggacg cgctggcgct ccaggccgcg ggggcgttct
     7321 cggtggtgct ggagatggtg cccgccgacg tcgcgaagcg ggtgacggcc gagctcgtca
     7381 tcccgacgat cggcatcggt gcaggcccgg acacggacgc ccaggtgctc gtctggtcgg
     7441 acatggccgg gatgaaccgc gggcgcaagc cccggttcgt gaagcagtac gccgacctcg
     7501 gcggggtcct gctggacgcc gccaagcggt tcggtgacga ggtgcgtggc gggatgtacc
     7561 ccgacgcgga gcactcgtac agctaggacc tcccgcacgg gcgcgggccc cgtagccgcg
     7621 cccgtgcggt gacccggctt cgccgcgtgg cgagccgggc ccgtgcgcac gacagggcgc
     7681 tcgcagtcga ctcgcgcagc gccaggcgtc tcgctcccag gaacgggact cgcgggggtt
     7741 ttccgggcga ctcgcggggg ctttccgggc gactcgcgcg caggaacggg cgactcgggg
     7801 gcgctttccg ggcgactcgc gcggcaggaa cgggcgactc gggggctttt ttcgggcgac
     7861 tcgcgggtca ggggtggcgc aggcctgcga ccggttcgcc gtcctcgtag cgggcgagca
     7921 tcgtcacggc ggggcactcg cgacgggccc agcgaccacg caggctctcc agccggccca
     7981 ggtcgtcgtc gcgcccggcc agcgctgccg ccgccgcgtc gaggagcgct ccggtgcagg
     8041 cgtggacgtc gtcgtccccg agcccggcga gcgcgacgtg ctggtgcagg gcggcgtccg
     8101 gggtggtgcg gcggccgggc agggtctcgt ccagcacgag cccggtgagc cagctgagca
     8161 gctccccgta cagcacgggg tcggggcagg cgtcgaacgc tttgaactcg atccgcccca
     8221 cctcggccgg tatccgcgcc tcctgcgtga gcgacgggtc ggcgtccacc tgctcgtcgc
     8281 gttcaccgag gaataccagc accgcgggac ggaggccggt gcggacagcg gtccgcgccg
     8341 agagaccacc ccacggcgca ccgtcccgga acggcgagga gaacgacagt ggcaccagcc
     8401 acgggctgta gtgcgtgagc ttgcgcccga tgtccatgag ggcggcggtg ccgagagtgg
     8461 cgcaggacag gttcaggtcg ggcccgtagg tcgacatgtg gaggtgcgcg gtgcgctcct
     8521 ccggcgagcc gagccggtgc tgccgctccc actcgttgag cggcggctcg atcctgtact
     8581 ccgagcgcag ggggttgaac ccgatcaccg tggtgcgcag ccgcgtagga gcgagcgcgc
     8641 cgtcgagccg ggccacgtcc gcgcggagcg cgcccacagc agcgtccacc gagccatgga
     8701 tccgggtacg gatctcgatg cccttggggt cacagcgcac cagccgccca tgggtgtcga
     8761 atcgctcgta gccctcgacg taccaacgct tgcgcttgat cccctggtca ccgacgcgca
     8821 actccgggta gtcgtccggg tcctccggga gcgcgtcgat gaccgcctgc agctcgtcga
     8881 atccggtgtc ggtgtagtcg gcgaagccgc cgtcgctgtg cagcaacgcg cactcgtgct
     8941 cgatgccgta gcggaacgct ctatcctcct ggacggcgcg gggctccacc gatcgacctc
     9001 ccgggatgtc ggcgaaccac tcgccgtgct gagggtagcc tcatcgcagc aacgagcgga
     9061 ggtggcggtt gatcggcgag gacccgctgg cagtgctgcg cgatcgacgc tcccgggccg
     9121 cactggcctc gcccgccccg ccccgaggtg agcttcagga gatcctggac gccgcctggt
     9181 cggtcccgga ccacgggcgg ctgcgcccgt ggcggttcat cgtcatcgag gaggcagcgc
     9241 gagatctgct cggcgacgcg ttcgccgccg cccacgcgga acgcgaaccg accgccgatc
     9301 ccgccgagct gcaccgcacg cgcaagaagc cgctgcgggc gccgctgatc gtcgtcgtgg
     9361 tcgcgacgcc gcgaccgcac ccgaaggtgc cggtctggga gcagcgggcc acggcggcgt
     9421 gcgtcgccta cagcgtcgtg ctcgccgcgc acgtccgcgg gctcggcgcg atgtggcgca
     9481 ccggctggta cggcgaggcg ccgaaggtgc gcgcccacct cgggctcgtc gacggcgagg
     9541 aggtgacagg ctgggtctac ctgggcaccc cggcagggcc cgagccgcca ccccgccccc
     9601 ccgcggatcc gcccgtcacc tggttcagct gacgccccgg acgccgccgc cctcagctga
     9661 cggcagcgaa ctcgatccgg cccgtggtcg ggtcggccac ggccagtcgc acccgcaccg
     9721 aggcacccgc cgtcagcggc ccggcgcagc gcgccagcac cggcgggtcg ggcaggtaga
     9781 cctcgcccag ctcgccctcc gggccgttcg gccgcagcgc cacaccgtcg aactcctccc
     9841 ccacgcgccc ggcgagcagc gcggccccgg tgcggtcgac acaggcacgg tcgacctttc
     9901 ccgcgagcgt gtcggacacg gtcatcacct ccggcagcgc cggcagggcc tcccgcagcc
     9961 acgacggcgg ctcgaggccg gcggtcactg ccaggcagag ctccgtgccg aaccggtcgg
    10021 ccagccgccg aagcggggcc gtgacgtgcg cgtagggcgc accgatcccg ccgtggcccg
    10081 cgtcgtcggg gaccggcaca cccgcgtccc ggtcgaacgc cgcgtagccc gcaccgcgca
    10141 gcaggatggt tgcggcgcgc cgcagcgcca gcgcggccgg ggcgtcgccc ggcaggtcgg
    10201 ccaggatctc ggccgccgtg ctgcccggcg gccacgtgaa gcccagctcg cgcgccgccc
    10261 ggcgaaaccc ctccagcgcc tccggctcca cggcgggcag ggtgcgtagc aggccgacgc
    10321 cggcatcgat catgatccgc gcggccgcca tccccgtgag cagcgagatc tccgcgttcc
    10381 acacctcgac gtcgagccgc cgccggacct ggacggtcca gccgccgagg ccgtccggaa
    10441 cgacctcctg ctcgggcagc tccagctcga tcacccctct ccgcaccgcc tggtggcggc
    10501 gcagcaggcc gagctcaggc agcgcgacca acgccgggtg cagccggccc gcctcggcgt
    10561 cggcctgcac acccgcatag tccagccgcg ccctggaccg gacgacggcc cggcgcacgt
    10621 cgaccgtcac cggctcgccc gcgtcgtcga ggtcgatcgt ccagagcacc gcgccgcgcg
    10681 agccgtcggg gagcaggctc gccgcgtcct cggacaacgt cggcgggtgc aggggcaccg
    10741 gcccatcggg caggtagatc gtctgccccc gcctgcgcgc ctccgcatcg agggcgccgc
    10801 ccggtgacac catcaccccg agatcggcaa tcgcgtagtg cacccggaag ccgccgccgc
    10861 gacgggagac gccgagcgcc tggtccaggt ccttcgaccc acgcgggtcg atcgtcacca
    10921 gctcgaggtc ggtggcgtcc acgcgctccg gacccgcggt cggtggccgc gaggccgccg
    10981 tggccgcctc ggccaacacc tccgccggga acgtgtcagg cagcgcgaac tcggcgcgga
    11041 cagcagcgaa ctgtggtgcg ccgcgcggcg ctcgcgtcgg gggcaggtgc acgcccggag
    11101 ccttccacgc aaccggtccg gtcgggaccg ctcctcagag ccagcgcggc agaacgggca
    11161 gcttccggcc ctccgtgctg ctgcgcagcg cacggccctt gccgcggccg aggagccact
    11221 cgagcaactc cggcgcaggc ccggagacgg tgatcgcatt ctcggcatcg cccaccgtcc
    11281 atgtcccgtc gggggcgacc aggcgcaggg atgggcagtc cgccttggcc cccatcgtcg
    11341 tagcgacctc ggtgagcaac tcggcaagca tcggcgccgg aaggtcggcg aacgaggcac
    11401 cgacgtcgag gtcgacggcg tggacccaca tctcccgcgc ccggatccag gggatgtcgg
    11461 tagccgggat ctcctggccc tgcacgttcc tcacctgggc cgaccat
//
LOCUS       DASRSC010000236         8759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4839569,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000236 DASRSC010000000
VERSION     DASRSC010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8759
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4839569"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1187)
                     /gene="ilvC"
                     /locus_tag="VFE39_11500"
     CDS             complement(183..1187)
                     /gene="ilvC"
                     /locus_tag="VFE39_11500"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET6259266.1"
                     /translation="MTVNIYYDDDADLSIIQGRKVAVIGYGSQGHAHALSLRDSGVDV
                     RIGLPEGSKSRAKAQDEGLRVVTPAEASAEADLIMILAPDTKQRFIYAEDIAPHLQPG
                     DAIFFGHGFNIRYELIKPPANVDVAMVAPKGPGHLVRRQFVDGKGVPCLIAVEQDPSG
                     NAKALALSYAAAIGGARAGVIETTFTEETETDLFGEQAVLCGGAAALVQTGFEVLTEA
                     GYAPEIAYFECLHELKLIVDLMYEGGIANERFSISDTAEYGDLTRGPRIITPAVKDEM
                     RKILAEIQDGRFAQEWVAEDEAGRPTFTRLQKEGAEHPIEQVGEKLRGLMSWVGTKAG
                     "
     gene            complement(1218..1724)
                     /gene="ilvN"
                     /locus_tag="VFE39_11505"
     CDS             complement(1218..1724)
                     /gene="ilvN"
                     /locus_tag="VFE39_11505"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET6259267.1"
                     /translation="MQRHTLSVLVEDKPGVLARVSGLFSRRGFNIESLAVGSTEHPDI
                     SRMTIVVAVDDFPLEQVTKQLNKLVHVIKIVELEPAASVQRELLLVKVRADPGVRSQV
                     LEIVNLFRAKAVDVTPEALTIEATGTADKLSALLKNLEPFGIREMVQSGMVAVGRGPR
                     SITAAAVR"
     gene            complement(1724..3676)
                     /locus_tag="VFE39_11510"
     CDS             complement(1724..3676)
                     /locus_tag="VFE39_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET6259268.1"
                     /translation="MTTATPRSGPVPAPAGPKPAPPPGRQGTHPGPSPVQANPVQAAP
                     GAHIDRVPMTGAQSLVRSLEEVGCEVVFGIPGGTILPAYDPLLDSTRVRHVLVRHEQG
                     AGHAATGYAQATGKVGVCMATSGPGATNLVTPLADAHMDSVPVVAITGQQTRPLIGTD
                     AFQEADITGITMPITKHNMLVTDAAEIPRAIAEAFHLASTGRPGPVLVDIPKDVLQEQ
                     TTFSWPPELKLPGYRPTTRPHGKQIREAAKLMCAAAQPVLYVGGGVMKANAEAELLEL
                     AELTDIPVVTTLMARGAFPDSHRQHVGMPGMHGTVTAVAAMQESDLLVALGARFDDRV
                     TGHLASFAPNAAIVHADIDPAEISKNRRADVPIVGDAKEVIAELVDALRIERAERGHT
                     DLTAWWSRLDGLRSTYPLGYDWPADGSLSPEYVIERIGQIVGPDALYAAGVGQHQMWA
                     AQFIRYEKPRTWLNSGGLGTMGYAVPAAMGAKMGRPDAVVWAIDGDGCFQMTNQELAT
                     CALEGIPIKVAIINNGNLGMIRQWQNLFYSERYSQSDLGTHKHRIPDFKLLAEAMGCV
                     GLRAESKDEVDRVIHQAMEINDRPVVIDFVVGADAQVWPMVAAGTGNSEIMAARNIRP
                     LFDGDELVAGVDEVAAVTAEALGDNR"
     gene            complement(3914..4717)
                     /locus_tag="VFE39_11515"
     CDS             complement(3914..4717)
                     /locus_tag="VFE39_11515"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6259269.1"
                     /translation="MITQSRVAPDVVALSAALPGPGGTMLPVNAYCLLGPEPVLVDTG
                     LSPLGEQFRSELWNLVDPADLRWVVVTHDDRDHTGSLRTILDQAPQATVVTNFISMVK
                     IGEDWPLPMDRLRLVNTGDRLGIGDDVLHVFRPPTYDSPGTLAFHAVHRSICFSSDCL
                     GAFLPDTVELGANAEDLPADDYAAGVAVFASALSPWMHDIAADRWHATLADLRRRDVG
                     TLLSTHGLAVRHGLGPLLDMAAGLPAGAAFVSPGQEFVDAVLSMSGNAH"
     gene            complement(4741..5511)
                     /locus_tag="VFE39_11520"
     CDS             complement(4741..5511)
                     /locus_tag="VFE39_11520"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HET6259270.1"
                     /translation="MTTSDTRRRAEPRRSVLPRVVAMQLAATEYGRLLDLLRDLGPDD
                     WSLRTDCTEWDVRELAAHNLGMAEMAASMRENLRQNAAARRRGGVFIDALTGLQVEER
                     RGMTPAALVERYAEVGPLAARGRRRTPGIVRRTAMPGTQPVGGSQERWTFGFLVDTIL
                     TRDTWMHRIDIARATRRDLVLTADHDGVLVGDVVSEWAGRHGSPCTLTLTGPAGGSWS
                     FGTGGPELELAAVEFCRIVSRRAGRPPGAGLLDVHVPF"
     gene            complement(5508..6152)
                     /locus_tag="VFE39_11525"
     CDS             complement(5508..6152)
                     /locus_tag="VFE39_11525"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6259271.1"
                     /translation="MLGETTTSWQAKRREGTRQEILSAAWAVAREHGLAALTLREVAA
                     RVGMRAPSLYSHFDSKHAIYDAMFEQAWSEYEELARESDATVPAEPRAALRYLAWRFF
                     DFSVADLARHQLMNVRTIPDFTPSAESYAPAVRVLDALHVRLAAIGITDGADIDLFVA
                     LVGGLIDHQWANDPGGTRWARLIDRAVDMYADALHLPRSDISSSDESATDQEER"
     gene            6221..6643
                     /locus_tag="VFE39_11530"
     CDS             6221..6643
                     /locus_tag="VFE39_11530"
                     /inference="COORDINATES: protein motif:HMM:NF022216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET6259272.1"
                     /translation="MSTAAGEGAEAPAPRVVFRVSPLVVLVGLVLAVCVTPVAGGAPY
                     LWLIYLAPIAVIVRTLRVRTVVDAEALTVRRIVGGRRVPWSQISSLRLGHGGRHDRTR
                     VSAMLTDGAELPLPAVHVRDLPQLAAASGGRLPDPAGE"
     gene            6649..8490
                     /gene="ilvD"
                     /locus_tag="VFE39_11535"
     CDS             6649..8490
                     /gene="ilvD"
                     /locus_tag="VFE39_11535"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET6259273.1"
                     /translation="MPALRSRVTTHGRNAAGARSLWRATGMTDDDFGKPIVAIANSYT
                     QFVPGHVHLKNLGDLVAEAIREAGGVPREFHTIAVDDGIAMGHGGMLYSLPSREVIAD
                     AVEYMVNGHAADALVCISNCDKITPGMLNAAMRLNIPTVFVSGGPMEAGKAVVVDGVA
                     QAPMDLITAISASASSVVDDEGLALVERSACPTCGSCSGMFTANSMNCLTEALGLALP
                     GNGSTLATHAARRELFLEAGRTVMALAKRWYAEDDASALPRSIATRKAFENAMALDVS
                     MGGSTNTVLHILAAAQEGEIDFDLEAIDTVSRRVPCLAKVAPNSDYHMEDVHRAGGIP
                     AILGELWRAGLLNEDVHTVHSPSMQQWLSEWDVRAEAPSEKAVELFHAAPGGVRTTEA
                     FSTENRWSSLDTDAAGGCIRDAEHAYTADGGLAVLRGNIAPDGAVIKTAGIPEDIWHF
                     SGPARVVESQEEAVSVILTKQIQPGDVLVVRYEGPAGGPGMQEMLHPTAFIKGAGLGA
                     KCALITDGRFSGGSSGISVGHISPEAAAGGVIGLIEDGDTVLIDVRSRTLEVQVADEV
                     LAERRAKMDSSERPWQPKDRDRAVSKALRAYAALATSAHTGAVRRVP"
     gene            complement(8542..>8759)
                     /locus_tag="VFE39_11540"
     CDS             complement(8542..>8759)
                     /locus_tag="VFE39_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259274.1"
                     /translation="PSQAYCPAVGAGSTAPELREVERWWAQAHVHVQYLGFAADHLGP
                     ARVAEMIETAASAMDRLGPALKSTTTR"
BASE COUNT         1248 a         3098 c         3103 g         1310 t
ORIGIN      
        1 cgccccgatg gtagatgcgc tgctcgccgg ggctcccagc ggcgtggccg cgactgacgg
       61 gacgaacggc acgaaaggga cgttgttccc gcgccttcgg gtgaaaatcc cggatcgccc
      121 acgaacggaa ctcccgtccg gacctgccgg acgggagtgc cgtcgtcatg cgtgcgggcg
      181 ggtcagcccg ccttcgtgcc cacccagctc atgaggccgc ggagcttctc gccgacctgc
      241 tcgatcgggt gctctgcacc ctccttctgg aggcgggtga acgtcgggcg ccctgcctcg
      301 tcctcggcca cccactcctg cgcgaagcgc ccgtcctgga tctcggcgag aatcttccgc
      361 atctcgtcct tgaccgcagg cgtgatgatg cgcgggccac gggtgaggtc gccgtactcc
      421 gccgtgtcgg agatggagaa acgctcgttg gcgatgccgc cctcgtacat gaggtcgacg
      481 atcagcttga gctcgtgcag gcactcgaag taggcgatct ccggcgcata gcccgcctcg
      541 gtgagcacct cgaacccggt ctggacgagc gcagcggcgc cgccgcagag cacggcctgc
      601 tcaccgaaca ggtcggtctc ggtctcctcc gtgaacgtgg tctcgatgac gcccgcgcgg
      661 gcgccgccga tggccgctgc gtaggagagg gccagcgcct tcgcgttgcc gctggggtcc
      721 tgctcgaccg cgatcaggca gggcacgccc ttgccgtcga cgaactgccg gcgcacgagg
      781 tgcccggggc ccttcggcgc caccatcgcg acgtcgacgt tcgccggcgg cttgatcagc
      841 tcgtagcgga tgttgaagcc gtgcccgaag aagatcgcgt cgcccggctg gagatgcggc
      901 gcgatgtcct cggcgtagat gaaccgctgc ttggtgtccg gcgccaggat catgatcaga
      961 tccgcctccg cggacgcctc ggcgggcgtc accacccgca ggccctcgtc ctgcgccttc
     1021 gcgcggctct tcgagccctc gggcaggccg atccgcacgt cgacgccgga gtcgcgcagg
     1081 gagagcgcgt gcgcgtggcc ctggcttccg tagccgatca cggcgacctt acggccctgg
     1141 atgatcgaca ggtcggcgtc gtcgtcgtag tagatgttga cagtcatagc tggtcagtgg
     1201 ttcctctcgg gtattgccta gcggacggcg gcggcggtga tcgaccgggg accacggccg
     1261 acggccacca tccccgactg caccatctcg cggatgccga agggctcgag gttcttcagt
     1321 agcgcgctca gcttgtcggc cgtaccggtg gcctcgatcg tgagggcctc cggcgtcacg
     1381 tccaccgcct tcgcgcggaa caggttgacg atctcgagca cctggctgcg cacgcccggg
     1441 tcggcgcgca ccttgaccag gagcagctcg cgctggacgg acgcagccgg ctccagctcc
     1501 acgatcttga tcacgtgcac gagcttgttg agctgcttcg tgacctgctc gagagggaag
     1561 tcgtcgacgg cgacgacgat ggtcatgcgg gagatgtcgg gatgctcggt ggagccgacc
     1621 gcgagggact cgatgttgaa gccgcgccgg gagaacaagc ccgagacccg ggcgagcacc
     1681 cccggcttgt cctccaccag caccgagagc gtgtggcgct ggatcatcgg ttgtccccca
     1741 gcgcctcggc ggtgacggcc gcgacctcgt cgaccccggc cacgagctcg tcaccgtcga
     1801 acagcggccg gatgttgcgg gccgccatga tctccgagtt gccggtgccc gcggcgacca
     1861 tgggccacac ctgcgcgtcg gcaccgacca cgaagtcgat cacgaccggc cggtcgttga
     1921 tctccatcgc ctggtggatg accctgtcga cctcgtcctt ggactccgct cggagcccga
     1981 cgcagcccat cgcctcggcg aggagcttga agtcggggat ccggtgcttg tgcgtgccga
     2041 ggtcgctctg ggagtaccgc tccgagtaga agaggttctg ccactgccgg atcatgccga
     2101 ggttgccgtt gttgatgatg gcgaccttga tcgggatgcc ctccagcgcg caggtggcga
     2161 gctcctggtt ggtcatctgg aagcagccgt cgccgtcgat ggcccacacc accgcatccg
     2221 ggcggcccat cttcgcgccc atcgcggcag ggacggcgta gcccatcgtg ccgaggccgc
     2281 cggagttgag ccacgtgcgc ggcttctcgt acctgatgaa ctgcgccgcc cacatctggt
     2341 gctgcccgac acctgcggcg tacagggcgt cgggaccgac gatctgcccg atccgctcga
     2401 tcacgtactc gggcgacagc gagccgtcgg cgggccagtc gtagcccagc ggataggtgg
     2461 agcgcagccc gtcgagcctc gaccaccacg cggtgaggtc ggtgtggccg cgctcggccc
     2521 gctcgatgcg cagcgcgtcg acgagctcgg cgatgacctc cttggcgtcg cccacgatcg
     2581 ggacgtcggc ccggcggttc ttggagatct cggccgggtc gatgtcggcg tgcacgatcg
     2641 ccgcgttggg cgcgaagctg gcgagatgac cggtgacccg gtcgtcgaac cgcgccccga
     2701 gcgcgacgag caggtcggac tcctgcatgg cggccaccgc ggtgaccgtg ccgtgcatac
     2761 cgggcatccc gacgtgctgg cggtggctgt ccgggaaggc accgcgggcc atcagcgtgg
     2821 tgaccacggg gatgtcggtc agctcggcca gctcgagcag ctcggcctcc gcgttcgcct
     2881 tcatgacgcc gccaccgacg tagagcaccg gctgcgccgc cgcgcacatc agcttggccg
     2941 cctcgcggat ctgcttgccg tgcggccggg tggtcggccg gtagccgggc agcttgagct
     3001 ccggcggcca ggagaacgtc gtctgctcct gcagcacgtc cttggggatg tccaccagga
     3061 ccgggccggg tcggcccgtg gacgccaggt ggaatgcctc cgcgatcgcg cgcgggatct
     3121 ccgccgcatc ggtcacgagc atgttgtgct tggtgatcgg catggtgatg ccggtgatgt
     3181 ccgcctcctg gaaggcgtcg gtgccgatca gcggtcgggt ctgctgcccg gtgatggcga
     3241 ccaccggaac cgagtccatg tgtgcgtccg ccagcggggt gacgaggttg gtggcacccg
     3301 gtcccgacgt cgccatgcag acgccgacct tgccggtggc ctgcgcgtac ccggtggcgg
     3361 cgtgccccgc gccctgctcg tggcggacca gcacgtgacg cacccgcgtc gagtcgagca
     3421 acgggtcgta ggcgggcagg atcgtgccgc ccgggatgcc gaagacgacc tcgcagccca
     3481 cctcctcgag cgagcggacg agcgactggg cgccggtcat gggcacccgg tcgatgtgcg
     3541 cgccgggagc ggcctgcacg gggttcgcct gcacggggct cgggcccgga tgggtgccct
     3601 ggcggcccgg gggcggcgcc ggcttcggtc ccgccggtgc ggggaccggg ccggaacggg
     3661 gcgttgcggt ggtcatatct gcggtcagct ccgcgggagt tgggtgtgga catgaaaaaa
     3721 cccccgaccg ccgggtggcg gaacgagggt ggcgcgtcga cgaccgagag ctcactcagg
     3781 cgtggacgcg cctgcaaagt acgagcacct cgacgacggg catgccttgg acgctagctc
     3841 cgatcagggc gaggcgtcaa acccctggga aactcgtacc gcatgatggg acgacatgca
     3901 gtgccacagc ggttcaatgg gcgtttcccg acatcgacag caccgcgtcg acgaactcct
     3961 gcccaggcga cacgaacgcc gccccggcgg gaaggccggc ggccatgtcc agcagcgggc
     4021 cgagcccgtg tcgaaccgcg agcccgtgcg tgctgagcag cgtgccgacg tcgcgccggc
     4081 gcaggtccgc cagcgtggcg tgccaccggt cggcggcgat gtcgtgcatc cacgggctca
     4141 gcgcggaggc gaagaccgcg acgcctgccg cgtagtcgtc ggcggggagg tcctcggcgt
     4201 tcgcgccgag ctcgacggtg tccgggagga acgcgcccag gcagtcggag ctgaagcaga
     4261 tgctccggtg gaccgcgtgg aacgccaggg tcccggggct gtcgtaggtc ggcgggcgga
     4321 agacgtggag cacgtcgtcg ccgatcccca gccggtcgcc ggtattgacc agccgcagcc
     4381 ggtccatcgg caacggccag tcctcgccga tcttcaccat gctgatgaag ttcgtcacca
     4441 cggtcgcctg cggcgcctgg tcgaggatcg tgcgcaggct gccggtgtgg tcgcgatcgt
     4501 cgtgcgtgac gacgacccag cgcaggtcgg ccgggtcgac caggttccac agctccgagc
     4561 ggaactgttc gccgagcggg gagagccccg tgtcgaccag caccggctcc ggcccgagca
     4621 ggcagtaggc gttgacgggc agcatcgtcc cgccgggccc cggcagcgcc gccgacaacg
     4681 cgacgacgtc cggcgcgact cgtgactggg tgatcatcgg ggtctcctcc cccattgagc
     4741 tcagaacggc acgtggacgt ccagcaaccc cgcgccggga ggcctgccgg cccggcggga
     4801 gacgatgcga cagaactcca cggcggcgag ctccagctcg ggcccgccgg tcccgaagga
     4861 ccagctgccg ccggcaggac cggtgagcgt gagggtgcag ggggacccgt ggcgcccggc
     4921 ccactccgag acgacgtcgc cgacgagcac cccgtcgtgg tcggcggtga gcaccaggtc
     4981 gcggcgggta gcccgggcga tgtcgatgcg gtgcatccag gtgtcgcggg tgaggatcgt
     5041 gtcgacgagg aagccgaacg tccaccgttc ctggctcccg ccgaccggct gcgtacccgg
     5101 catcgccgtc cggcgcacga tcccgggggt acgacgccgt ccgcgcgccg ccaacgggcc
     5161 gacctccgcg taccgctcga cgagcgcggc cggggtcatc ccgcggcgct cctcgacctg
     5221 cagcccggtg agcgcgtcga tgaacacccc gccgcggcgg cgggccgccg cattctgccg
     5281 gaggttctcg cgcatcgagg ccgccatctc ggccattccg aggttgtgcg cagccagctc
     5341 gcggacgtcc cactcggtgc agtccgtccg gagcgaccag tcgtcgggcc cgaggtcacg
     5401 cagcaggtcg agcaggcgcc cgtactcggt cgccgccagc tgcatcgcca caacccgagg
     5461 aaggaccgag cggcggggct ccgcccgtcg gcgtgtgtcg gatgtcgtca tcgttcctcc
     5521 tgatccgtgg cggactcgtc gctactggaa atgtcactgc ggggcagatg gagtgcgtcg
     5581 gcgtacatgt cgacggcgcg gtcgatgagc cgggcccagc gggtgccccc tgggtcgttc
     5641 gcccactggt ggtcgatcag gccgccgacc aacgcgacga acaggtcgat gtcggcgccg
     5701 tcggtgatgc cgatcgccgc gagccggacg tggagcgcgt cgagcacgcg taccgcgggg
     5761 gcgtagctct cggcgctcgg ggtgaagtcg gggatcgtgc gcacgttcat cagctggtgg
     5821 cgggccaggt cggcgaccga gaagtcgaag aacctccagg cgaggtaccg cagagcggca
     5881 cgcggctcgg cggggaccgt cgcgtcgctc tcccgggcca gctcctcgta ctcgctccag
     5941 gcctgctcga acatcgcgtc gtagatcgcg tgtttggagt cgaaatgcga gtacagcgac
     6001 ggtgcacgca tcccgacccg cgccgcgacc tcgcgcagcg tcagcgcggc gagcccgtgc
     6061 tcgcgcgcca ccgcccaggc agccgacagg atctcctggc gcgtcccctc acgccgtttg
     6121 gcctgccaac tcgtggtagt ttctcctaac acgattagga ccttcctcct gcccaccacc
     6181 gctgtcaacg ggcagccggg catcggggga agatggctgc atgagcactg ctgcaggcga
     6241 aggcgccgag gcccccgctc cccgtgtcgt cttccgcgtg tccccgctcg tcgtgctcgt
     6301 cgggctcgtc ctcgccgtct gcgtcacgcc ggtggcgggc ggggcgccgt acctctggct
     6361 gatctacctc gcgccgatcg ccgtgatcgt ccggacgctg cgggtgcgca ctgtcgtcga
     6421 cgccgaggcc ctgaccgtgc gccggatcgt cggcggtcgg cgcgtaccgt ggtcacagat
     6481 cagctcgctg cggcttggcc acgggggtcg gcacgaccgc acccgggtca gcgcgatgct
     6541 gaccgacggt gccgagctac cgctccccgc cgtccatgtc cgtgacctgc cccagctcgc
     6601 cgccgccagt ggcggccggc tgcccgaccc ggctggagag tgagcccgtt gccggctctg
     6661 cgttcccgcg tcaccaccca cggccgcaac gccgccggag cacgttcgct ctggcgcgcc
     6721 accggcatga ccgacgacga cttcggcaag ccgatcgtcg ccatcgccaa ctcctacacc
     6781 cagttcgtgc cagggcacgt gcacctaaag aacctcggcg acctcgtcgc ggaggcgatc
     6841 cgcgaggcgg gcggcgtgcc gcgggagttc cacaccatcg ccgtcgacga cggcatcgcg
     6901 atgggccacg gcgggatgct ctactcactc cccagccgcg aggtgatcgc cgacgccgtc
     6961 gagtacatgg tcaacgggca cgccgccgac gcgctggtct gcatctccaa ctgcgacaag
     7021 atcacccccg gcatgctgaa cgccgcgatg cgcctcaaca tccccaccgt cttcgtctcc
     7081 ggcggcccga tggaggcggg caaggcggtc gtggtcgacg gcgtcgccca ggcgcccatg
     7141 gacctgatca ccgccatctc ggcctccgca tcgagcgtgg tggacgacga aggactcgcg
     7201 ctcgtggagc gttccgcctg cccgacctgc ggctcgtgct cgggcatgtt cacggcgaac
     7261 tcgatgaact gcctcaccga ggcgctcggg ctcgcactgc ccggcaacgg ctccaccctg
     7321 gccacgcacg ccgcgcgccg cgagctgttc ctggaggccg gtcgcacggt gatggcgctc
     7381 gcgaagcgct ggtacgcgga ggacgacgcc tccgctctgc cccgctcgat cgccacccgc
     7441 aaggcgttcg agaacgcgat ggcgctcgac gtctcgatgg gcggctcgac gaacaccgtg
     7501 ctgcacatcc tcgcagcggc acaggagggc gagatcgact tcgacctgga ggccatcgac
     7561 acggtgtcgc ggcgggtgcc gtgcctggcc aaggtggcgc cgaactccga ctaccacatg
     7621 gaggacgtgc accgggccgg tggcatcccg gccatcctgg gtgagctgtg gcgggccggg
     7681 ctgctcaacg aggacgtgca cacagtgcac tccccgtcga tgcagcagtg gctctccgag
     7741 tgggacgtcc gcgccgaggc gccgtcggag aaggccgtgg agctgttcca cgccgccccg
     7801 ggcggggtgc gcaccacgga ggcgttctcc acggagaacc gctggtcctc gctcgacacc
     7861 gacgccgcgg gcggctgcat ccgcgacgcc gagcacgcct acaccgccga cggcgggctg
     7921 gccgtgctgc gcggcaacat cgcccccgac ggcgccgtca tcaagaccgc cggcatcccc
     7981 gaggacatct ggcacttctc cggccccgcc cgggtcgtgg agagccagga ggaggccgtg
     8041 tcggtcatcc tcaccaagca gatccagccc ggtgacgtgc tcgtggtgcg ctacgagggc
     8101 cctgcgggcg ggccgggtat gcaggagatg ctgcacccca ccgcgttcat caagggtgcc
     8161 ggtctcggcg ccaagtgcgc gctgatcacc gacggccggt tctccggtgg gtcgagcggc
     8221 atctcggtcg ggcacatctc ccccgaggcc gctgccggcg gggtgatcgg cctcatcgag
     8281 gacggcgaca ccgtgctgat cgacgtccgg agccggacgc tcgaggtgca ggtcgccgac
     8341 gaggtcctgg ccgagcgacg ggccaagatg gactcctccg agcgcccctg gcagccgaag
     8401 gaccgcgacc gcgccgtctc gaaggcgttg cgcgcctacg cggcgctggc cacgagcgcg
     8461 cacaccggag cggtccgccg ggttccctaa ggcccgtgcg cggatacgag tgctccggca
     8521 ctcgtatcca cgcacgacct ctcagcgtgt ggtggtcgac ttcagcgccg ggcccaggcg
     8581 gtccatggcg ctcgccgcgg tctcgatcat ctcggccacc cgcgccgggc ccaggtggtc
     8641 ggcggcgaag ccgaggtact ggacgtggac gtgcgcctgc gcccaccacc gctcgacctc
     8701 gcgcagctcc ggagctgtcg accccgcacc caccgccggg cagtacgcct gcgatggct
//
LOCUS       DASRSC010000237         9933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3527634,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000237 DASRSC010000000
VERSION     DASRSC010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9933
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3527634"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..1211)
                     /locus_tag="VFE39_11545"
     CDS             complement(216..1211)
                     /locus_tag="VFE39_11545"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HET6259275.1"
                     /translation="MTVARSGRRRTVLIGAVAVLAVLVAGIAVVLAKPDGGGSAGITA
                     VAPAYPAPGMLEKGPAADAKGDYPRSGVAGERAQSAPAPDGAPGPGAPFGSVQRALVR
                     TAQVTVEVADPAISARQLRTAAAGVSAFVTQEQSSDTGSWLVLRVPADALDRFIEDIA
                     AFGRVLGRSTQVLDATEEAVDLDARVASQQASVTRVRSLLAEATSIGDVVAIESELSR
                     READLDSLTSRLEALRDQVALSTLTVDLRAPGVPPAQNRPAPGFRDGLATGWEGLRAL
                     GSGAAAVIGFVLPFLPLLALLGGIAWLARRIIRARRTPAAAGAGGRGGPGSEGES"
     gene            complement(1339..1797)
                     /locus_tag="VFE39_11550"
     CDS             complement(1339..1797)
                     /locus_tag="VFE39_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HET6259276.1"
                     /translation="MAAALAELDADHHRRGPGQFLVTLPGTNRLQTHCWLLVREHSLF
                     VQAFVCRQPDEDHDAVYRFLLQRNARLYGVHYALDRIGDIHLIGRVGLHAVTTAELDR
                     VLGQVLEAADGDFNTLLELGFASSIRREHAWRQERGESLANLKAFEHLFT"
     gene            complement(1841..3154)
                     /gene="mshA"
                     /locus_tag="VFE39_11555"
     CDS             complement(1841..3154)
                     /gene="mshA"
                     /locus_tag="VFE39_11555"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HET6259277.1"
                     /translation="MTIASDRAVRRPRRVCLLSVHTSPLEQPGTGDAGGMNVYIVETA
                     TRMAQRGVDVEIFTRATSSENPPVVELAPGVLVRHVAAGPFEGLGKNDLPSQLCAFTA
                     GVLRAEARHEPGYYDVVHSHYWLSGQAGWLARDRWGVPLVHSAHTLARVKNAALADGD
                     QPEPMVRVIGEDQVVAEADRLIGNTDEETRQLVELYGADPHRTVTVAPGVDLARFTPG
                     KRAASRQVLGLAPDAVVLAFVGRIQPLKAPDVLVRAAAEMLRRDPALRSRLVVLVAGG
                     PSGSGLTEPTALQELGASLGITDVLRFLPPQTRQGLVHVYRAADVVAVPSHNESFGLV
                     ALEAQACGTPVVAARVGGLSVAVDHGRSGLLVPGHSAEQWADALGAVALVPGRRDELG
                     RAATRHARGFSWDRTAEALLHTYAEAATEYAERQGLVVRASIPAG"
     gene            complement(3185..6157)
                     /locus_tag="VFE39_11560"
     CDS             complement(3185..6157)
                     /locus_tag="VFE39_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6259278.1"
                     /translation="MTAPSFGGARVDIDPFGTGALRAAVLDSWAGSPTRFREDANAEE
                     DLRLGGYADAWFVELAQNAADAARSAGRSGRLRVTVTDGELRVANTGAPLDAAGVAAL
                     ASLRASAKRDDPGSVGRFGVGFAAVLAVSDAPRVVSSDGGVAFSAVRTAEAVAALPGP
                     AAELARRDEPPVLRLVWAADERPPDGFDTEVRLPLRPGLDVHALLAQARETAPDLLLA
                     LPDLVEIAVGDDVLLRVDGPVPGEVTIGTRRWLLARRTGTLQADDARSQATEQRSRRD
                     WSACWALPLTAAGLPDPLSGDVLHAPTAAVERLALPARLIATVPLEPDRRRVRPGPAT
                     DRVLAGAAQAYLDLVRAVPPVQRLALVPEPGFPRSELDGRLRDLLLDALRAAAWLPGV
                     TGAELVPGRAEWLDLPGADQPDARLPALLAEAGFDRLLAPASMSVSPRSVRAPTALSE
                     LGVHRLTAAELVTRLFGVERPPQWWRALYAELAPIAETVPGLVDELRALPVPLADGRV
                     AAGPATVLLPPVDGAEAGAVEHIAALALPGLHVADPAAAHPLLTRLGAAVADPAGLLE
                     HPALAEAVERSVDDAEAGLDTRPLAEAVLELVAELGSAPDGLAALALPDRDGRPARAD
                     ELMLPDAALRPLLAADVPLDVLHEGFAARVPRSALTAVGVVDGFAVLVDEDPVAPDHD
                     LDDEECWWDSLDTPPARLVAVRDLDLVAENAWPAALALLAAERETRAALTAPGSYTSW
                     WLTRHARLGGRRPGHWRLPTAHGLAALYDPPPGAGDSDDSGGGVVDEAVLSAIGVRAD
                     LAISDASVAADLMARLADPARHPDVALVAEAHAALAEAVAVDRVHPADLDMPEQVRAL
                     DGSVAEVDVAMVLDAPWLAAVLPAGELVTGGDPAALADLLDLPVASEIVAAEVEGTGR
                     AVRWAELAEVVVACHTLGAPVPAGELTVHDELWVAVRRPVSGRFRVPTWRDATGQWHA
                     ADPVRALLGLLAEDDVPG"
     gene            complement(6157..6933)
                     /locus_tag="VFE39_11565"
     CDS             complement(6157..6933)
                     /locus_tag="VFE39_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3027 domain-containing protein"
                     /protein_id="HET6259279.1"
                     /translation="MNVVPTLDPPTRLVDAVEQARSAAIDQAAADMGGRAALDAVGEH
                     LEAVSEGPGGVTHYFAARQGGYRGWRWSVTLASGGEDTPITVSEVVLLPGPDALVAPA
                     WVPWGQRIRPGDLGVGDLLPTEEGDERLVPGYLASDDPAVEEVVLEVGLGRPKVLSHR
                     GRELAAQRWFEGTHGPASDMARAAPAPCGTCAFFLPIAGSLRGAFGVCGNEYSPADGA
                     VVTVGFGCGAHSDVQVEPVSPVAVAELVYDDGVDLEPVEK"
     gene            6961..7740
                     /locus_tag="VFE39_11570"
     CDS             6961..7740
                     /locus_tag="VFE39_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019816673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminyl-peptide cyclotransferase"
                     /protein_id="HET6259280.1"
                     /translation="MAFGRALVGALVLIVAGCAAASPAPDLAPGAPVLRPEVLAELPH
                     DPTAFTQGFELADGGLYEGTGLAGRSQLRELDPVSGAVRRSVPLPGRLFGEGITVVGD
                     RIWQLTWRDDVVLEWDRSTFTLIRELPLADEGWGLCHDGTRLVRSDGTDMLHFQDPTT
                     FAETGSIAVTLDGRPVTELNELECADGQVWANVWQTDRIVRIDPATGRVTAVVDAAGL
                     LDAGRRAHADVLNGIAALGNGEFLLTGKLWPVTFRVRFVPR"
     gene            8026..9792
                     /locus_tag="VFE39_11575"
     CDS             8026..9792
                     /locus_tag="VFE39_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259281.1"
                     /translation="MGQNDQVARSESSPPRRGRRGGRRTWVQGDGERATSVTPPPPGT
                     EPPQRPLRQDVETPSPPTAPERQPYEARRRYPWHDDPDYDPAAHRRTPSPSWPPPASD
                     QGTNAADASDEEPRTEAAPPAAPGETVGEDDRPTPRMPSKLTVTRVAAFRGRQLTQSG
                     IRAFRRAAGADGADRSGLTALTYATMATYAVDAAVAVALANTLFFAAATAESKANVAL
                     YLAITVAPFAVVAPVIGPLLDRLQRGRRAALAVSFAGRAVLAVVMALNYDTWVLYPAA
                     LGALVLSKSFSVLKAAVTPRVLPTAITLVTTNSRLTAFGLAAGAVFGGIAAGVATLAG
                     SPGALLYTAALSVVGTVLCLRIPRWVESTAGEVPAALRTTRRGRRSPMGRGAVVALWG
                     NGALRILTGFLTLFVAFVVKGQTEADPGRQLLLIGIVGAAAGVGSFAGNAVGSRRRFG
                     RSDRVVLGCIVAAVASAVVAAVLPGIATAALVGLVGASASALGKVCLDAVIQRDLPEE
                     SRASAFGRSETVLQLAWVFGGALGVLLPHSTFWIGFAVVAAVVTLAGVQTALVSRGRS
                     LLPFIGHRVHPGATPQPARTVR"
BASE COUNT         1341 a         3771 c         3594 g         1227 t
ORIGIN      
        1 gaaccacagc aaccaagtgg ggcggtagtt gcatcgaggc cgcgccggag tggaaccacc
       61 ggcgccggcg aggccagtgc gaagcgctca cgcggaaggc aaatcagcgg atcggccgaa
      121 cgagacacct gagcgccggc agccttccag tcgtcgagca cccacgcgga ccgcggaggc
      181 accatgacgc tctgaccaag tagtttccgc cggtctcacg actccccctc agatcccggg
      241 ccaccccgcc cacccgcacc tgccgcagcc ggcgtccgcc gggcccggat gatccgccgg
      301 gccagccagg cgattccgcc cagcagggcg agcagtggca ggaacggcag tacgaacccg
      361 atcaccgcgg ccgcaccgga tccgagggcg cgcagccctt cccatcccgt cgcgagcccg
      421 tcgcggaagc ctggagcggg ccggttttgc gccggcggca ccccgggcgc cctcaggtcg
      481 accgtcaacg tcgacagcgc cacctggtcg cgcagcgcct cgagcctgct cgtcagtgag
      541 tccagatcgg cttcccggcg ggacagctcg gactcgatgg ccaccacgtc gccgattgac
      601 gtggcctcgg cgagcagcga ccgcacccgc gtgacgctcg cctgctggct cgcgacccgc
      661 gcgtcgaggt cgacggcctc ctcagtggcg tcgaggacct gggtgctgcg ccctaggacc
      721 cgcccgaacg cggcgatgtc ctcgatgaac cggtcgagcg cgtcagccgg cacccgcaga
      781 accagccagc tgcctgtgtc gctcgactgc tcctgcgtga cgaatgcgct caccccggcc
      841 gccgccgtac ggagctgccg ggcggatatc gccggatcgg ccacctcgac agtgacctgc
      901 gcggtgcgga ccagcgcacg ttgcacggag ccgaacggtg cacctggtcc tggcgccccg
      961 tccggcgccg gagccgactg ggctcgttct ccggcgacgc cggacctggg gtagtcgcct
     1021 ttcgcatccg cggcggggcc cttctccagc atgccgggcg ccggataggc aggcgcaacg
     1081 gcggtgatcc cggccgaccc gccgccatcg ggcttggcca gcaccacagc gataccggcc
     1141 acgagtaccg cgagcaccgc caccgctccg atcagcaccg tcctgcgcct gcccgaccga
     1201 gccaccgtca ccccctcctc gccccggtgc cccggggttc cggcaggagg acggtggccg
     1261 cgggcgcgga ggttggtcgc ccaaggtcac gatcgcgtca cgcacgccgg acgggccgct
     1321 cgacccccgg ttcggtcgtc aggtgaacag gtgctcgaag gccttcaggt tggcgaggct
     1381 ctccccacgt tcctgccgcc aggcgtgctc ccgccggatc gacgaggcga agcccagctc
     1441 gagcagcgtg ttgaagtcgc cgtcggcggc ctcgagcacc tgtccgagca cgcggtccag
     1501 ctcggcggtg gtgacagcgt gcaggcccac ccgcccgatc aggtggatgt cgccgatgcg
     1561 gtccagcgcg tagtgcacgc cgtagagccg ggcgttgcgc tgcagcagga accggtagac
     1621 ggcgtcgtgg tcctcgtcgg gttggcggca cacgaaggcc tgcacgaaca gcgagtgctc
     1681 gcggaccagc agccagcagt gcgtctgcag ccggttggtt ccgggcagcg tcacgaggaa
     1741 ctgtccgggg ccgcgccggt ggtggtcggc gtccagctcc gccagcgcgg ccgccaccgc
     1801 cgcgtcggcg gcatcgacgt cctcggtgct cacggctgca tcatcccgcc gggatgctcg
     1861 cacggacgac gagcccctgg cgctccgcgt actccgtggc cgcttccgca taggtgtgca
     1921 gcaaggcctc ggccgtccga tcccaggaga acccgcgcgc gtgccgcgtg gccgcgcgac
     1981 ccagctcgtc gcgtcgaccg ggaacgagcg ccaccgcgcc cagcgcgtcg gcccactgct
     2041 ccgcactgtg gccgggcacg agcagcccgg accggccgtg gtccacggcg accgacagcc
     2101 cgccgacgcg cgccgcgacc accggggtac cgcaggcctg cgcctccagc gccaccagac
     2161 cgaaggactc gttgtggctc ggcacggcca ccacgtccgc agcccggtag acgtgcacca
     2221 gaccctgccg ggtctgcggg ggcaggaagc gcaggacgtc ggtgatcccg agcgaggcac
     2281 cgagctcctg cagcgccgtc ggctcggtca ggccgctgcc cgacgggccg ccggcgacga
     2341 gcacgacgag tcgggagcgc agtgccggat cacggcgcag catctcggct gccgcgcgca
     2401 ccagcacgtc gggagccttg agcggctgga tgcgcccgac gaacgcgagc acgaccgcgt
     2461 ccggggcgag accgaggacc tgccgggacg cggcgcgctt gcctggggtg aaccgggcaa
     2521 ggtccactcc gggcgcgacg gtgacggtgc gatgcgggtc ggcgccatag agctcgacga
     2581 gctggcgcgt ctcctcgtcg gtgttgccga tgagtcggtc ggcctcggcc accacctggt
     2641 cctcaccgat cacccgcacc atcggttccg gctggtcgcc gtcggccaac gcggcgttct
     2701 tgacgcgggc aagcgtgtgg gcgctgtgca cgagcgggac cccccagcgg tccctggcca
     2761 accaaccggc ctgcccggac agccagtagt gcgaatgcac cacgtcgtag tagccgggct
     2821 cgtgtcgggc ctccgcgcgc agcacgcccg cggtgaaggc gcacagctgg ctcggcagat
     2881 cgttcttgcc gagcccttcg aacggcccag ccgccacatg gcggacaagc acgccgggcg
     2941 ccagctcgac caccggcggg ttctccgacg acgtcgcgcg ggtgaagatc tcgacgtcca
     3001 caccgcgctg tgccatcctc gtggcggtct ccacgatgta gacgttcatg ccgccggcgt
     3061 caccggtgcc cggctgttcc aggggcgagg tgtgcacgga cagcaggcag acccgacggg
     3121 gtcgccgcac cgcacggtcg ctggcgatgg tcacacggcc ctgtgtagca cgactcgaga
     3181 cgactcagcc gggcacgtcg tcctcggcca gcaggccgag cagggcccgc accgggtcgg
     3241 ccgcatgcca ctgtccggtc gcatcacgcc aggtcgggac ccggaaccgg ccgctcaccg
     3301 gtcgccgcac ggccacccac agctcgtcgt ggacggtgag ctcaccggcc ggcaccggcg
     3361 ccccgagggt gtggcaggcc accacaacct cggcgagctc cgcccagcgg accgcccgtc
     3421 ccgtgccctc cacctccgcg gccacgatct cggaggccac gggcaggtcg agcaggtcgg
     3481 ccagcgcggc ggggtcgccg cctgtcacca gctcgcccgc cgggagcacc gccgccagcc
     3541 acggcgcatc gagcaccatc gccacgtcga cctcggcgac gctgccgtcg agtgccctca
     3601 cctgctccgg catgtcgaga tccgccgggt gcacgcggtc gacggcgacc gcctcggcca
     3661 gcgccgcgtg cgcctcggcc acgagcgcga cgtcggggtg ccgggccggg tcggccagcc
     3721 gggccatcag gtcggcggcc acggacgcgt cggagatggc gaggtccgcc cgcaccccga
     3781 tcgcggagag cacggcctcg tcgaccaccc cgccgcccga atcgtcgctg tcaccggcgc
     3841 ccggcggcgg gtcgtagagc gccgccaggc cgtgcgccgt agggaggcgc cagtggccgg
     3901 gtcggcggcc gccgagccgg gcgtggcggg ttagccacca ggaggtgtac gaaccgggag
     3961 ccgtgagcgc ggcccgggtc tcgcgttcgg cagcgagcag ggcgagcgct gcgggccacg
     4021 cgttctctgc gacgaggtcg aggtcccgga cggcgacgag ccgcgccggc ggggtgtcga
     4081 gcgagtccca ccagcactcc tcgtcgtcga ggtcgtggtc gggggccacc ggatcctcgt
     4141 cgacgagcac cgcgaagccg tccaccacgc cgaccgcagt gagcgccgat cgcggcacgc
     4201 gcgccgcgaa accctcgtga aggacgtcga gcgggacgtc cgcagcgagc aggggacgca
     4261 gcgcggcgtc gggcagcatc agctcgtcgg ctcgggccgg gcggccatct cgatcgggca
     4321 acgccagcgc ggcgaggcca tccggtgcgc tgccgagctc agcgaccagc tccagcaccg
     4381 cctcggcgag tgggcgggtg tccagcccgg cctccgcgtc gtccacggac cgttccacgg
     4441 cctcggcgag cgcggggtgc tcgagcaggc cggccgggtc ggcgacggcg gcaccgagcc
     4501 gggtgagcag cgggtgcgcc gccgccgggt cggccacgtg caggccgggc agggcgagcg
     4561 ccgcgatgtg ctcgacggct cccgcctcgg cgccgtccac gggcggcagg agcaccgtcg
     4621 ccgggccggc ggccaccctg ccgtcggcaa gcgggacggg gagcgcccgc agctcgtcga
     4681 ccaggccggg gaccgtctcg gcgatggggg ccagctccgc gtagagcgcc cgccaccact
     4741 gcgggggacg ctcgacgccg aacagccggg tcaccagctc cgctgcggtc agccggtgca
     4801 cacccagctc ggagagcgcc gtcggcgccc gaacagaacg gggcgacacg gacatcgacg
     4861 ccggtgccag cagccggtcg aacccggcct cggcgagcag tgcgggcagc cgtgcgtccg
     4921 gctggtccgc gccgggcagg tccagccact ccgcacggcc cggtacgagc tccgcgccgg
     4981 tgacgccagg cagccaggca gcggcgcgca gagcgtcgag cagcaaatca cgtagccgcc
     5041 cgtccagctc cgaccgcggg aaccccggct cgggcacgag tgcgagccgc tgcacgggcg
     5101 ggaccgcccg gacgaggtcg aggtaggcct gcgccgcgcc ggccagcacc cgatcggtgg
     5161 ccgggccggg gcgcacgcgg cggcgatcgg gctccaacgg cacggtggcg atcagccggg
     5221 caggcagcgc gagccgctcg acagccgccg tcggcgcatg caggacgtcg ccggagaggg
     5281 ggtcggggag gccggcggcg gtgagcggca gcgcccagca ggccgaccag tcgcgccgcg
     5341 accgctgctc ggtggcctgc gatcgggcat cgtcggcttg gagcgtgccg gtgcgccggg
     5401 cgagcagcca gcgccgggtc ccgatcgtca cctcaccggg caccggaccg tcgacgcgga
     5461 gcaggacgtc gtccccgacc gcgatctcca cgaggtcggg aagcgcgagc agtaggtctg
     5521 gcgcggtctc gcgggcctgc gcgagcagcg cgtgcacatc gaggccggga cgcagcggca
     5581 gccgcacctc ggtgtcgaac ccgtcaggcg gccgctcgtc ggctgcccac accaaccgca
     5641 gcacgggcgg ctcgtcacgg cgggccagct cggccgccgg tccgggcagc gcggcgaccg
     5701 cttcggccgt gcggaccgcg gagaacgcga ccccaccgtc gctactgacc acgcgcggag
     5761 cgtcggacac agccagcacg gccgcgaagc cgacgccgaa ccggccgacc gaacccgggt
     5821 cgtcccgctt ggcggacgcg cgcagtgaag ccagcgcggc caccccggcc gcgtccagcg
     5881 gagccccggt gttggccacc cgcagctcgc cgtcggtgac cgtgacgcgg agccgtccgg
     5941 agcggccagc ggaccgtgcg gcgtcggcgg cattctgggc aagctcgacg aaccacgcgt
     6001 cggcgtaacc gccgaggcgc aggtcctcct ccgcgttggc gtcctcgcgg aagcgcgtgg
     6061 gcgaaccggc ccaggaatcc agcactgcgg cgcgcagcgc gcccgtgccg aacggatcga
     6121 tgtcgacgcg cgctccgccg aaactgggtg cggtcactac ttctcgaccg gctccaggtc
     6181 gaccccgtcg tcgtagacga gctcagccac cgccaccggc gagaccggct cgacctggac
     6241 atcggaatgg gccccgcagc cgaagccgac ggtgacgaca gctccgtcgg caggcgagta
     6301 ctcgttgccg cacacgccga aggcgccacg gagcgaaccg gcgatcggca ggaagaacgc
     6361 gcaggtccca cacggagccg gggcggcgcg ggccatgtcc gaggccggtc cgtgcgtccc
     6421 ctcgaaccag cgctgtgcag caagctcgcg gcccctgtgg gagagcacct tcggcctgcc
     6481 gaggccgacc tcgagcacga cctcctccac cgcagggtcg tcggaggcga gatacccggg
     6541 cacgagccgc tcgtcgccct cctcggtcgg cagcaggtcg cccacgccga ggtcgcctgg
     6601 ccggatccgc tgcccccacg gcacccaggc cggtgccacg agcgcatccg ggccgggcag
     6661 caggacgacc tcactgaccg tgatcggcgt gtcctcacct ccggaggcga gcgtgaccga
     6721 ccaccgccag ccgcggtatc caccctggcg ggcagcgaag tagtgcgtga cgccacctgg
     6781 cccctcggag acggcctcga ggtgctcccc gacagcgtcg agggcggcgc ggccgcccat
     6841 gtcggcagcg gcctggtcga tggcggcgga ccgggcctgc tccaccgcgt ccacaagccg
     6901 ggtcggcggg tcgagggtcg gtacgacatt caccacaggg attgtgccac cctccccgcc
     6961 atggctttcg ggcgtgcgct tgtcggggcc ctggtactga tcgtggccgg ctgcgccgca
     7021 gcgtccccgg cgccggacct ggcccccggg gcacctgtgc tccgaccgga ggtgctggcc
     7081 gagctgccgc acgaccccac cgcgttcacg caggggttcg agctcgccga cggtggcctc
     7141 tacgagggca ccggtctcgc cgggcgctcg cagctgcgcg agctcgaccc ggtgagtgga
     7201 gccgtccgtc ggtcggtccc actgccgggc cggttgttcg gagagggcat caccgtggtg
     7261 ggcgaccgca tctggcagct cacgtggcgc gacgacgtgg tgctggagtg ggatcgcagc
     7321 acgttcacgc tcatccgcga gctgcccctc gcggatgagg gctgggggct gtgccacgac
     7381 gggaccaggc tggtgcgcag cgacggcacc gacatgctgc acttccagga ccccaccacg
     7441 ttcgccgaga ccggctcgat cgccgtcacc ctcgacggcc gaccggtgac cgagctcaac
     7501 gagctggagt gcgcggacgg gcaggtctgg gccaacgtct ggcagaccga ccggatcgtg
     7561 cgcatcgatc ccgccaccgg ccgcgtcacg gcggtggtcg acgccgccgg gctactggac
     7621 gccggacgcc gggcgcacgc ggacgtcctg aacggcatcg cggcgctggg caacggtgag
     7681 ttcctgctca ccgggaagct ctggcctgtg acgttccgcg tccggttcgt cccccgctga
     7741 cccgcgagcc gcccacccag tcccgcgagt cgccccattt ccgcccccga gtcgcccgcc
     7801 cgagtcccgc gagtcgcccg gtttccgccc ccgagtcgcc ccgccccgca cgcgagtcgc
     7861 ctcctcccgc acgcgagtcg cccccgtctc ggcacgcgag tcggccctct ccgcttgcgg
     7921 gtcggccgtc ccgacacacg cgagtcgccc gtcccggctg cgtcagtcgc ccgatctgat
     7981 catcgtggcc cgcctcccgt gtgacactca tcgcagagcg tccagatggg ccagaatgac
     8041 caggtggccc gctccgaatc gtccccgccc cgccgcggcc gtcgtggggg gcgccggacg
     8101 tgggtccagg gcgacggcga gcgggccacg agtgtgacgc caccgccccc cggcaccgag
     8161 ccgccgcagc gccccctgcg tcaggacgtc gagacaccga gccccccgac cgcgcccgag
     8221 cggcaaccgt acgaggcgcg ccggcgctac ccctggcacg acgacccgga ctacgaccct
     8281 gcggcacacc gccgcacgcc gtccccgtcg tggccgccgc ccgcctccga ccagggcacc
     8341 aacgcggcgg acgccagtga cgaggagccc aggacagagg cggcgccacc ggccgccccc
     8401 ggggagaccg tgggtgagga cgatcggccc acgccccgga tgccgagcaa gctcaccgtc
     8461 acgcgcgtgg cggctttccg cggccggcag ctgacccaga gcggcatccg ggcgtttcgc
     8521 cgggccgccg gtgccgacgg cgccgaccgt tccggcctca ctgcgctcac ctacgccacg
     8581 atggcgacct acgcggttga cgcggctgtg gccgtcgcgc tggccaacac cctgttcttc
     8641 gcagcggcca cggcggagag caaggccaac gtcgccctct acctggccat cacggtggca
     8701 ccgttcgccg tggtcgcgcc ggtgatcggg ccactgctcg accggttgca gcggggtcgc
     8761 cgcgcggcgc tggccgtgtc gttcgccggc cgagccgtgc tcgccgtggt gatggcgctc
     8821 aactacgaca cctgggtgct ctatccggct gcactcggcg cactggtgct cagcaagtcg
     8881 ttctccgtac tcaaagcggc ggtcacgcca cgcgtcctgc cgacggcgat cacgctcgtg
     8941 acgacgaact cccggctgac ggcgttcggg ctggccgcgg gcgccgtctt cggtggaatc
     9001 gcggcgggcg tcgccacgtt ggccggttcg ccgggcgcac tcctgtacac ggccgcgctg
     9061 tcggtcgtcg gcaccgtgct gtgcctgcgg atcccgcggt gggtggagtc cacggcgggc
     9121 gaggtgccgg ccgcgctgcg caccaccaga cggggcaggc gcagcccgat gggccgcgga
     9181 gccgtggtcg cgctgtgggg caacggcgcg cttcgaatcc tcaccgggtt cctcacattg
     9241 ttcgtcgcgt tcgtggtgaa gggtcagacg gaggccgatc ccggccgaca gctgctcctg
     9301 atcgggatcg tcggggccgc tgcaggcgtg ggctcgttcg ccggcaacgc cgtcggctcc
     9361 cggcgccggt tcgggcggtc cgaccgcgtc gtgctggggt gcatcgtggc cgccgtcgcc
     9421 tccgccgttg tcgccgccgt gctgcccgga atcgccacag cggccctcgt cggcctggtc
     9481 ggcgcctcgg cgagcgcgtt gggcaaggtc tgcctggacg ccgtgatcca gcgcgacctg
     9541 cccgaggagt cgcgtgcgtc ggcgttcggc cgctcggaga cggtgctgca gctggcctgg
     9601 gtgttcggcg gcgcgctggg tgtgttgctt ccgcacagca cgttctggat cgggttcgcg
     9661 gtggtcgctg ccgtggtcac gctggcgggc gtccagacgg cgctggtcag ccggggacgc
     9721 agcctcttgc cgttcatcgg gcaccgggtg catccggggg ccacaccgca accggcccgc
     9781 accgtccgct gaggcgccga ccgtagggtt gacgggtgcc gcgccgactc ctgctgggcc
     9841 gactcctgct gggccgactc ctgctgggcc gaccccggtc ggcccgtctc cctctgggcc
     9901 gcctggtccc actaccggtg gcggcgctcc tcc
//
LOCUS       DASRSC010000238         4934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_581889,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000238 DASRSC010000000
VERSION     DASRSC010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4934
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581889"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VFE39_11580"
     CDS             complement(<1..473)
                     /locus_tag="VFE39_11580"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6259282.1"
                     /translation="MSKQSSVERAARAAERFGSAADAVDAAAADLLGVNRTDLRILGA
                     VVERALTAGQVADTVGLSPAAATTAIQRLVARGHLTREPDPDDRRRAVVELTPSAREQ
                     AKLIYGPVGEAGVAELHRFTAAELEVIADFLERGRALQLAQAERIRGMAGARDQE"
     gene            526..993
                     /locus_tag="VFE39_11585"
     CDS             526..993
                     /locus_tag="VFE39_11585"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HET6259283.1"
                     /translation="METQQLPSWLPTANRIVRAVHRIGLPLGTIHLLTVPGRRTGRPR
                     ATPVSPLVVDGHRYLVAGLPQSDWARNARAAGRGELVRGRRRRDVALREVTDPDLRRA
                     VMRAFPTEVPHGVDFFVRIGLVERADPEQFAAAAERVAVFELGDTPGDTAARP"
     gene            complement(1013..1210)
                     /locus_tag="VFE39_11590"
                     /pseudo
     CDS             complement(1013..1210)
                     /locus_tag="VFE39_11590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332412.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
     gene            complement(1340..2260)
                     /locus_tag="VFE39_11595"
     CDS             complement(1340..2260)
                     /locus_tag="VFE39_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HET6259284.1"
                     /translation="MLRSALLAASRSRAVRAAVEAGPFDLLVNRFVAGTTVEDALTAA
                     RRITADRLVTVDHLGEDTTDRAQADATVAAYRTLLGRLSHEGLADRVEVSVKLSALGQ
                     SLAVDADKIALESARQICESAAAAGTTVTVDMEDHTTTESTLGIVRDLRVDHPWVGAV
                     LQAQLRRTEGDCRDLAGGGSRVRLCKGAYDEPASAAFRRGEVDRSFVRCLKVLMAGEG
                     LPMVATHDPRLVEIARFLAERHGRTADDYELQMLYGVRPHAQSELAAEGLRVRVYLPY
                     GTEWFGYFMRRLGERPANLAFFARSLLTRG"
     gene            2357..3988
                     /locus_tag="VFE39_11600"
     CDS             2357..3988
                     /locus_tag="VFE39_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6259285.1"
                     /translation="MDSTLRQLLAAVGEPLVDVVEVPAGLDVPVTGLAIVDPDDEPDQ
                     YTGQLVLVIGARGRDAVAIVRTLARGRAAAVAVKAGGAVDELRAAAAGAGVALLVVRP
                     DVRWERLESLARDVLDSGGDTGEDGPGDLFSLAQTVALLTHGIVSIEDTASRVLAYSR
                     SDSDAAQVDELRRLSILGWQGPPGYLRMLREWGVFDRLRTGEEVVHVDEHADLGIRRR
                     LAVGIHAGQRQLGTIWVQEGATPFAERAADVLVGASRVAAGHLIRRRSRQAPGARWSR
                     DLVAGLLDGRTNPGLVAGMFGLDEHGSALVVAFAVRDRGSLPAGDRATYELSLAELTD
                     VVSVHAATYRRAALTASHDARVYAVLPDVPRDRAEPALLAMCTEVAAMTLRRTGVRVQ
                     AGIGSTVAGLADVGASRGEADRVLDAMASGPEDSTVAAFADLRAEVLLNQTLGLLQAS
                     PELRDPAVALLVGYDRDHGTDLVGSVVAWLDAMGDVRVAAQRLTVHPNTLRYRVRRAV
                     AVSGLRLDDPRARLMHHLQLLAVRSDKRKPASSSG"
     gene            4051..>4934
                     /locus_tag="VFE39_11605"
     CDS             4051..>4934
                     /locus_tag="VFE39_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009075672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6259286.1"
                     /translation="MDAITSTPKPRNEPVRDYGPASPERASLQAALAELSGQHHDLTV
                     TIDGRQHMAGGTRIDVVAPHDHAHVLGTTANAGHEDAKVAIEAALRAGPAWRALPFDE
                     RAAVLLRAADLLAGPWRDRLNAATMLGQSKTAIQAEIDSACELADFWRFNVHFARQIH
                     ENQPNSGTGAWNRMDYRPLEGFVYAITPFNFTAIAGYLPTAPALMGNTVIWKPSPTQG
                     LAAHLTMRLLEEAGLPPGVINLLTGDGRDISEVLLADRALAGIHFTGSSATFQHLWRQ
                     VGGSIGSYATYPRLVGETG"
BASE COUNT          695 a         1771 c         1807 g          661 t
ORIGIN      
        1 aactcctgat cccgcgctcc tgccatcccc cggatccgct cggcctgcgc cagctgcagt
       61 gcccggccgc gctcgaggaa gtcggcgatc acctcgagct cggccgccgt gaagcgatgc
      121 agctcggcca cccccgcctc accgacgggc ccgtagatca gcttcgcctg ctcccgggcc
      181 gacggcgtca gctcgacgac ggcgcgcctg cggtcgtcag gatcgggctc tcgcgtgagg
      241 tggccgcgag ccacgagccg ctggatggcc gtggtggccg cggcggggct gagccccacg
      301 gtgtcggcca cctgccctgc ggtgagcgcc cgctccacca ccgcgccgag gatgcgcaga
      361 tccgtgcggt tgacgccgag caggtcggcc gctgccgcat ccacggcgtc ggcagcactg
      421 ccgaaccgtt cagcggcacg ggcggcgcgc tccacggagg attgcttcga cactcgaagg
      481 agtctactct cggctcatta cttcgacggt cgaagggatt ctcccgtgga gacacagcag
      541 ttgccgtcct ggctcccgac agcgaaccgc atcgtgcggg ccgtgcaccg gatcgggctg
      601 ccgctcggca cgatccatct cctgaccgtt cccggccgcc ggacgggacg gccacgcgcc
      661 acacccgtgt cgccgctcgt cgtggacggg caccggtacc tcgtcgcggg gctgccgcag
      721 tccgactggg cgcgcaacgc tcgggccgcc ggccgcggcg agctcgtgcg gggcaggcga
      781 cgccgggacg tcgcgctgcg tgaggtgacc gaccccgacc tccgccgagc ggtgatgcgc
      841 gccttcccga cggaggtgcc gcacggggtg gacttcttcg tgcgcatcgg gctcgtcgaa
      901 cgagccgacc ccgagcaatt cgccgcggcg gccgagcggg tggccgtgtt cgagctcggc
      961 gacaccccgg gcgacaccgc ggctcgacca tgacgcgacg cccctgcgcg cgtcaggcct
     1021 gagcgtcgct cgaccgcgac agcgccgacc tgcgcctgct gtatgcgaag tagacgacca
     1081 gcccgatcag gaaccagacg gcgaaccgca tccaggtgtc cgggctcagg aagctgatca
     1141 gccacagcga gaacaccacg ccgatcgcgg gcaccaccgg catgccgggg cagcggaacg
     1201 agcggggcag gtagacggtc gcggcgccgg tgaacgcgcc ggtcagctcg aacggcgtga
     1261 acgggttgta gttgcccgcg tcgatgtgga agaacccgac cggcggtcgt gccatctcca
     1321 accgtgctga aacagctgct cagccgcggg tcagcagcga gcgcgcgaag aatgccaggt
     1381 tggcggggcg ctcgccgagc ctgcgcatga agtagccgaa ccactcggtg ccgtaaggca
     1441 ggtagacgcg cacccgcagc ccctcggccg cgagctcgga ctgcgcgtgg ggccgcacgc
     1501 cgtagagcat ctgcagctcg tagtcgtcgg ccgtgcggcc gtgccgctcg gccaggaagc
     1561 gcgcgatctc gaccagtcgc gggtcgtggg tggccaccat cggcagaccc tcgcccgcca
     1621 tcaggacctt caggcaccgg acgaaggaac ggtcgacctc cccgcgacgg aacgcggccg
     1681 acgccggctc gtcgtaggcg cccttgcaca gccggacccg cgagcccccg cctgcgaggt
     1741 cgcggcagtc gccctcggtg cggcgcagct gcgcctggag cacggcgccg acccagggat
     1801 ggtcgacgcg gaggtcgcgc acgatcccca atgtcgattc ggtggtggtg tggtcctcca
     1861 tgtcgacggt gacggtggtg cccgccgcgg cggccgactc gcagatctga cgcgccgact
     1921 cgagcgcgat cttgtcggcg tccaccgcga ggctctggcc gagcgcggag agcttcaccg
     1981 acacctccac gcggtcggcg aggccctcgt gactgagccg ccccaacagg gtccggtatg
     2041 cggccacggt tgcgtcggcc tgcgcacggt cggtggtgtc ctcgccgagg tggtcgacgg
     2101 tcacgagccg gtcggcggtg atccggcgag cagccgtgag ggcgtcctcc acggtcgtgc
     2161 ccgccacgaa gcggttcacc agcaggtcga acgggccggc ctcgacggcg gcacggacgg
     2221 cccgggaccg ggaggcagcg agcagggcgc tccgaagcat gcgcaggacc gtagatcgcg
     2281 tccgcaccgt tcggacttgt ccgatcagat aaggttcggg cacttcgctc gtccgagccg
     2341 atgatgaggc cgcaccgtgg acagcaccct gcgccagctc ctggccgcgg tcggcgagcc
     2401 actggtcgac gtggtggagg tgcccgcagg gctcgacgtg cccgtcacag gcctcgccat
     2461 cgtcgatccc gacgacgagc cggaccagta cacgggacag ctcgtactgg tgatcggcgc
     2521 ccgcggccgc gacgccgtgg cgatcgtccg cacgctggcg cgtgggcgcg cggcggccgt
     2581 cgccgtgaag gcgggcggtg cggtggacga gctgcgcgcc gcggctgcag gcgcgggggt
     2641 cgcgctgctg gtcgtgcgcc cggacgtgcg gtgggaacgg ctggagtccc tcgccaggga
     2701 cgtgctcgac agcggcggcg acaccgggga ggacggcccc ggcgacctgt tctcgctggc
     2761 ccagacggtc gcgttgctca cgcacggcat cgtcagcatc gaggacacgg ccagccgagt
     2821 actcgcctac tcccgctcgg acagcgacgc cgcgcaggtc gacgagctgc ggcggctgtc
     2881 gatcctgggc tggcagggcc cgccgggcta cctgcgcatg ctgcgcgagt ggggggtgtt
     2941 cgaccggctc cgcacgggcg aggaggtggt gcacgtcgac gagcacgccg acctcggcat
     3001 ccgccggcgg ctcgccgtcg gtatccacgc ggggcagcgg cagctgggga cgatctgggt
     3061 gcaggaaggc gccacgccgt tcgccgagcg ggccgcggac gtcctcgtcg gtgcgtcgcg
     3121 ggtggcggca ggccacctga tccggcggcg cagccggcag gcgccgggcg cgcggtggag
     3181 cagggacctc gtggccgggc tactggacgg gcggacgaac ccggggctgg tcgccggcat
     3241 gttcgggctg gacgagcacg ggagcgcgct cgtggtcgcg ttcgccgtcc gcgaccgcgg
     3301 ctcgcttcca gcgggtgacc gcgcgaccta cgagctgagc ctggccgagc tcaccgacgt
     3361 cgtctcggtg cacgccgcca cctaccgccg ggccgcgctc accgcatcgc acgacgcacg
     3421 ggtctacgcc gtgctgcccg acgtgccgcg ggaccgggcc gagcccgcgc tgctcgcgat
     3481 gtgcaccgag gtggcagcaa tgacactgcg ccgcaccggc gttcgcgtgc aggccgggat
     3541 cgggtcgacc gtggccggtc tggccgatgt gggggcgtcc cgcggcgagg ccgaccgggt
     3601 gctggacgcg atggcgagcg gccccgagga ctccaccgtg gccgccttcg ccgacctccg
     3661 ggccgaggtg ctgctcaacc agaccctcgg gctgctgcag gcgagcccgg agctgcgtga
     3721 tcccgccgtc gcgctgctgg tcgggtacga ccgggaccac ggcaccgatc tcgtcggctc
     3781 ggtggtcgcg tggctcgacg caatgggtga cgtgcgggtg gcggcgcagc ggctcacggt
     3841 ccaccccaac acgctgcgct accgggtgcg gcgggcggtg gcggtcagcg ggctgcgcct
     3901 ggacgacccc cgggcccggt tgatgcacca tctgcagctg ctcgctgttc gatcggacaa
     3961 gcgcaagccg gcatcgtcat ccggctgaac gaaccgtccc ggtcggttgg ttcctagggt
     4021 tctgggcacc tgacccgggg aggcttcgcc atggacgcga tcaccagcac gccgaagccg
     4081 cgcaacgagc ctgtgcgcga ctatggaccg gcctcgcctg agcgggcgag cttgcaggca
     4141 gcgttggccg agctcagtgg gcagcaccac gacctgaccg tgacgatcga cggccgccag
     4201 cacatggcgg gtggcacgcg catcgacgtc gtcgccccgc acgaccatgc ccacgtactg
     4261 ggcaccacgg ccaacgcggg ccacgaggac gccaaggtcg cgatcgaggc ggccctgcgg
     4321 gccggcccgg cgtggcgcgc gctgccgttc gacgagcgcg ccgctgtgct gctgcgggct
     4381 gccgacctgc tcgcgggacc gtggcgcgac cggctcaacg ccgcaacgat gctcggccag
     4441 tcgaagacgg cgatccaggc agagatcgac tccgcctgcg agctcgccga cttctggcgc
     4501 ttcaacgtgc acttcgcgcg tcagatccac gagaaccagc cgaacagcgg gaccggcgcg
     4561 tggaaccgca tggactaccg cccgttggag ggcttcgtct acgcgatcac cccgttcaac
     4621 ttcaccgcga tcgccggcta cctgcccact gcgcccgcgt tgatggggaa cacggtgatc
     4681 tggaagccgt cgccgacgca gggcctcgcc gcgcacctca cgatgcggct gctcgaggag
     4741 gcaggcctgc caccgggcgt gatcaacctg ctcacggggg acggtcgcga catctccgag
     4801 gtgctgctcg ccgaccgagc actcgccggc atccacttca ccggctcgtc ggccacattc
     4861 cagcacctgt ggcggcaggt cggggggagc atcggctcct acgcgaccta cccccgactg
     4921 gtcggtgaga ccgg
//
LOCUS       DASRSC010000239        14221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5333247,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000239 DASRSC010000000
VERSION     DASRSC010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 14221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14221
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5333247"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VFE39_11610"
     CDS             complement(<1..491)
                     /locus_tag="VFE39_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016696932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HET6259287.1"
                     /translation="MRYLITGATGYIGGRLAARLIDAGAGTVRCLVRDPDKLRDVPWA
                     HLAEIMQGDLLDPASIRAACEGVDVLYFLVHSLSDRDFAATDRRGALITGQAAREAGV
                     RRIVYLGGLHPDGALSEHLASRTEVGEILLRSGVPTAVLQAAVIIGSGSASFEMLRYL
                     TERL"
     gene            719..2872
                     /locus_tag="VFE39_11615"
     CDS             719..2872
                     /locus_tag="VFE39_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259288.1"
                     /translation="MVGDAASVLADALAAGRELDLQGAALSATTLAAVLTDAPPPGAP
                     SLRLRRANITGELRLTGATVGIPVELRGCVFAHVPDLRMAQFAGLALTGCRLPGLRAG
                     NLRVTADLLLDDGFTAHGPVHLADAQIGGSLRLSGGRLRGAGGRALIGDRMVVDGTCY
                     ARRLRSSGEVRLPGARITGNLDLGGADLSSPTGDALDATGVTVGGSLLAGRHNAGPDV
                     AFCASGRVLLAGSRVGGDLVLSGARIERSGAAAARDPEPAGESRMPVVPGGIVDGTAC
                     LVADRIRVEGNLELDDGMHTDGTVRLPNAVVGGYLRLSGARLTGPAGAMERGIALLGD
                     GMDVSGDLEGRDNGRGPLVCAGQLRLVDAHVRGSASLSGVELATPGGYALLADRLRIG
                     GEFYLRRLRCAGTVRLQNAEIGATLDCTGARLEHPRLRPDGTPRPSLDARAATIGKDM
                     ICTEGFTAAAGVRVRRMEARKSVQFVDATLGTTSTAGIRYALNAYGLVTAELVLRPAA
                     APAGRVRLAQAQVSTFADSAPLWAAVGGIDLDGFDYQKMNDTHVIDIQTRLHWLEQVM
                     PDYAAGPYEQLAAAYRRAGDEELSERVLMARQSRRYAEAGVAGRIWGALQRWTVGFGY
                     QPWLAVCWLVLFAVLGGAWFATHAPPPVDEGQNPVFNPWLFAADTLLPIVNLGQDGYW
                     RLDGASQWIASALVAAGWILATTAAAGAARILKRV"
     gene            3051..3431
                     /locus_tag="VFE39_11620"
     CDS             3051..3431
                     /locus_tag="VFE39_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259289.1"
                     /translation="MSPATRYSTTDDDWNPATGRHAFNEDAVLTPIFHALTRGGWRSR
                     QHEPAAPALPPVPTAPPRAPDPVDAFRRDPLTAPIPIQTLAPAAIPVTPSSGTGAHAV
                     PAARHELVDQGRHHRRREAVTSHW"
     gene            3786..4742
                     /locus_tag="VFE39_11625"
     CDS             3786..4742
                     /locus_tag="VFE39_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259290.1"
                     /translation="MFPTAASTAGACLRRDAVRLLGEKRVRTALSSGEFGSPFPGVLV
                     DPARAAEPSTVIAAGWLASGPDAIITGSTGAFLHGYTAAAPTPVHVVVPYGSRKRTRP
                     GLVVHNGRSLDFDRRFVDGLPVLCVERIVTDLVCSAAPADALAVVDQVLAALAPAERA
                     PFRDELHRRLRERADPRGTRIGTRLLDLATGRADSPAESWLLWRIVDLGFPVPIINYQ
                     VCTIDGEPRYRVDLGWPDLRIAIEYYGHAAHAERTEEDEARILELERRGWIVIIVRAE
                     DLAHMSRVEREVDEAFRRRGVYLRERTVGALRARRHRDLRAS"
     gene            complement(5305..6627)
                     /locus_tag="VFE39_11630"
     CDS             complement(5305..6627)
                     /locus_tag="VFE39_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET6259291.1"
                     /translation="MTSLGVRLTAPVVLPCDPGCSVLRDAVVDIDAGGRITHVGPRSS
                     APPTDAPVRALPGALVPGLINTHAHTPMSVLRGMGGDLPLLSWLYDVMWPAEGRLDAA
                     DVRAGMTAGTVELLRTGCTTSVEMYFFMDAVIEAVTAVGSRVVLTPGIIAAPGWDRLG
                     TWQQMRDEVSARIDAVGLRSGPGERIELGYGPHGAYTLPPEALESVGEHARERGALVH
                     MHVAEAANEDLAQREAYGSVPALLDRVGMLGGRVLAAHGVQLSDDDIALLAARGTAVA
                     HCPGSNAKLAAGIARVSALRRAGVRVGLGTDGPASGDDLDLWAEARLAGLLARVTSGD
                     AAALTAAELLLMATRDGAAAIGRDDIGALEPGRWADLVHVDLSDSAFVAPEDDAQLLS
                     NLVWAGGSRLVRDVWVAGEQVLADREPTRVNRAATTADLRKVAARLHT"
     gene            complement(6624..7250)
                     /locus_tag="VFE39_11635"
     CDS             complement(6624..7250)
                     /locus_tag="VFE39_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HET6259292.1"
                     /translation="MTAAIPPDPAPRVMLVWDAPNMDMSLGSLLGARPTAAFRPRFDA
                     VGRWLLELAGPDAIAEATVFTNVVPGSTEVVRPWVEALRNVGFAVFAKPKITDDSDVD
                     EDMLAHIRRRAEEGVLRHVVVASGDGRAFREPLEELEKQGTSVTVIGFREHASFALTS
                     EVIEFIDLEDIEGVFREPLPRITLDSLPDTGAWLPPFRSLRSLLEARR"
     gene            7414..7845
                     /gene="paaI"
                     /locus_tag="VFE39_11640"
     CDS             7414..7845
                     /gene="paaI"
                     /locus_tag="VFE39_11640"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HET6259293.1"
                     /translation="MTDHESDDVAARTAKAMHRADAAGRGAGVRLLDVGPGRARVAMT
                     VESRHVNGHGICHGGYLFLLADAALAYASNSYGTSAVASGADIAFLHPVPLGAELVAE
                     AVERARTGRSGLYDVTVRSGEQAVAEFRGRTRQVPGLPPPG"
     gene            complement(7868..9058)
                     /locus_tag="VFE39_11645"
     CDS             complement(7868..9058)
                     /locus_tag="VFE39_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016335598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HET6259294.1"
                     /translation="MTSLVATNVGVPAPRSAERCPATGAVTAPPQAAPDAVDPGEAEQ
                     FLTQFYAETSPSGSIDYRMWQVRHEIDSTGSYTHTVDELTYGARVAWRNSARCIGRLY
                     WNSLRLRDRRHVTAPADVAAECVEHLRDATRGGRIRSTITIFAQDRPDRPGPRIHNDQ
                     LVRYAGHRTTSGEIRGDSRYAGFTDRAVELGWQRPEPPGRFDVLPLLVSDGGGEPEMF
                     DVPADAVLEVPLIHPDHEWFAELRLRWHAVPAISNMPLVVGGVTYPAAPFNGWYLGTE
                     IGARNLVDADRYNLLPAVADRLGLDMSSERTMWRDRALVEMVRAVQYSFDVAGVTMAD
                     HHSESQRFLTHLAREESAGRRCPADWSWIVPPVSGGLTPVYHRYYDEPDASTRPAFLQ
                     PRSS"
     gene            complement(9122..9832)
                     /gene="trmB"
                     /locus_tag="VFE39_11650"
     CDS             complement(9122..9832)
                     /gene="trmB"
                     /locus_tag="VFE39_11650"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET6259295.1"
                     /translation="MHSFVHHRSRLTDGQQHAWERWWPSRGRDVADLLSGAEPFDPPA
                     WFGRSAPLILEIGSGMGESTAALAAAAPDVDHIAVEVFEPGLAQLLMRMADAGLSNVA
                     LLRGDAVALLHECVPAASLAGIRIFFPDPWPKRKHHKRRLVQPDFVSLAASRLVPGGI
                     LHMATDWDDYACQMRDVCDAEPFLENAAADQPGGWTPRPEWRPVTKFEQRARIEGRDV
                     RDLLYRRAFTGPSSGHSA"
     gene            complement(9861..10946)
                     /locus_tag="VFE39_11655"
     CDS             complement(9861..10946)
                     /locus_tag="VFE39_11655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6259296.1"
                     /translation="MSTPAVTATVVICVYTEKRWGDIVAAVDSVAAQDVTAAETLVIV
                     DHNAALLSRAQEAFTTRGVRVLPNAHKQGLSGARNTAVAEATGDVVVFLDDDATARPG
                     WLAALLAPYSDPTVAAVGGVAHPRWPARGVRAGTARPHVLPGADPGNGDATGELDWIV
                     GCTYTGQPTEQAEVRNLMGCNMSFRRDVFERVGGFAEDIGRIGKNPLGCEETELCIRA
                     RQAYRRVGEKIRIVFEPRAIVDHRVSEDRVEWAYLRRRSWAEGLSKAAVSALVGSDDA
                     LSTERSYVAKVLPTAVLRELRQRNVVSAAAVVTALAWTSAGFLRGKLPGATAGVRLPA
                     AQSDSGARVDTAREGAPAAPEHPNVGG"
     gene            complement(10986..11711)
                     /locus_tag="VFE39_11660"
     CDS             complement(10986..11711)
                     /locus_tag="VFE39_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6259297.1"
                     /translation="MVPTRNEARNLEIILPAIAAVRPAVHEIIVVDGNSIDGSIEAAK
                     RVLPSVRTITQTRKGKGNAMACGFAAATGDVIVMFDADGSADPAEIPAFVSALIAGAD
                     FAKGSRFTTGGGSDDITLLRKTGNAGLNGVANALFGTAYTDLCYGYNAFWADLLPVLE
                     LPPVDAPPPAEGMLWGDGFEIETVLNCRIAAAGLKITEVPSIERLRMFGETNLRTFAD
                     GARVLRTLLAEHRRADRHTSDRR"
     gene            12021..13934
                     /locus_tag="VFE39_11665"
     CDS             12021..13934
                     /locus_tag="VFE39_11665"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HET6259298.1"
                     /translation="MSTETVDPHVALAAELARLGRRLDEMGAELLRLHTDAAPVAAGP
                     APDAGTAPWSRSGDARPGAQVASNVDSAARSALDAPGGIAAGQGTPAPYWTPVPQPSG
                     PAEARPYPLVTRRLTWTAISGARLLAWTGGGVTLLGVVLLLVLAASRGWFSPPARVTV
                     GALLGVALIGLALWLHRRESARAGALALAATGFATLYLVVAAATALYDYLPDVPALLV
                     ALLVAAAGLGLADRWRAQLLGGGVVVGAALLAPVIVTDWLLVALALALQLAALPVVLR
                     RGWSVLMLVAAAGPVIFGSAVGAMVVVDGDVVTIAVVLGVLAVGLGTAMPAARMLPRE
                     PVAGLVAATPVPVLATGAALGGWDGGALTAVAALAMGGFAAVPGLDRLLRTVGVTAAA
                     VALFQATLVAVEGSTATAVLIGQAIVAAVVAAVLRSRLPMMIALAYGTVGVLMGLAQD
                     APVAALVQFPAIPYDQPGPGALLTGVGVSALALVLAVALLVAGGRVELIRPDTASAPL
                     WIPIGLVGLYGATSLMVTIALLVSTDRAGFTGGHAVVTVSWTIAALVLLARGISRAAL
                     RITGLVLVAAAVAKLVLFDLVALDGVARVAAFLGAGLVLLAAGTRYARLVAEAEAEAS
                     MEREAQGAPGNAS"
     gene            14009..>14221
                     /locus_tag="VFE39_11670"
     CDS             14009..>14221
                     /locus_tag="VFE39_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259299.1"
                     /translation="MPDRYIDAVRARRVELRAWRGLLTGGHRAELRSCLALSRIAAAA
                     EASGALAGLAREAADHIETGDRAARGQ"
BASE COUNT         1924 a         4991 c         5260 g         2046 t
ORIGIN      
        1 aggcgctcgg tgagatagcg caacatctcg aagctcgccg agccggaccc gatgatcacc
       61 gccgcctgca gcaccgcggt cggcacgccg ctgcgcaaca ggatctcgcc cacctcggtg
      121 cgggacgcga ggtgctcgga cagcgccccg tcgggatgca gtccgcccag gtagacgatc
      181 cggcgcacgc cggcttccct ggcggcctgc cccgtgatga gcgcgccgcg gcggtcggtg
      241 gcggcgaagt cccggtctga cagcgagtgc acgaggaagt agaggacgtc gacgccctca
      301 cacgccgcgc ggatgctcgc ggggtcgagg agatcgccct gcatgatctc cgcgaggtgc
      361 gcccacggga catcccgcag cttgtcggga tcgcgaacca ggcagcggac ggtgcccgcg
      421 ccggcatcga tcagccgcgc ggccagccga cccccgatgt acccggtcgc gccggtgatg
      481 aggtaacgca ccccccgatg ctcccggcag ccgcggattg ccgctacttc acggcccgcg
      541 accatttcag ccgggggacc actcgcaccc cgtgactcac ccgctcgcgg cggctcggcg
      601 ccggctgggt ggttcaggtt cacccaggac aggctgatct ttacccgcga agatcctttc
      661 ggttcgcaac gtcgtggtag acactccgaa tactcgtgga tcatgggagg ccggcgctgt
      721 ggtgggggat gcggcgtcgg tgctcgccga tgcgcttgcg gccggccggg aactcgatct
      781 ccagggcgcc gcgctgtcgg caacgaccct cgcggccgtc ctcaccgacg cgccaccgcc
      841 aggtgcaccg tcgctgaggc tgcggcgcgc caacatcacc ggcgagcttc ggctcaccgg
      901 agccaccgtc gggattcccg tcgaactgcg tggttgcgtg ttcgcgcacg tccccgacct
      961 gcggatggcg cagttcgccg gactcgcgct caccgggtgc cggcttcccg gcctccgggc
     1021 aggcaacctg cgcgtgactg ccgacctgct gctcgacgac gggttcaccg cacacggacc
     1081 ggtccacctc gccgacgcgc agatcggcgg gtcgctgcgg ctgtcggggg gacggctgcg
     1141 cggagcgggc ggccgagcgc tgatcggcga ccgcatggtc gtcgacggca cgtgctacgc
     1201 gcggcggctg cgcagcagtg gggaagtccg gttgcccggg gcgcggatca ccggcaacct
     1261 cgacctcggt ggggccgacc tgagcagccc gacgggggac gccctcgacg ccaccggcgt
     1321 cacggtcggc ggcagcctcc tcgcaggccg gcacaacgcc ggaccggacg tggcgttctg
     1381 cgcgtcgggc cgggtgctgc tcgccggttc cagggtcggg ggcgacctcg tgctgtccgg
     1441 ggcccgcatc gagcggagcg gggccgccgc cgcacgcgac ccggaaccgg cgggggagag
     1501 tcggatgccc gtcgtcccgg gcgggatcgt cgacggcacg gcgtgcctcg tggccgaccg
     1561 catccgggtg gagggcaacc tcgaactcga cgacgggatg cacaccgatg gcaccgtgcg
     1621 gctcccgaac gccgtcgtcg gcggctacct gcggctctcc ggtgcccggc tgaccggccc
     1681 tgcgggcgcg atggagcgcg ggatcgcgct gctcggggac ggcatggacg tcagcggcga
     1741 cctcgagggc cgcgacaacg gccgcggccc gctcgtctgc gctggtcagt tgcgcctggt
     1801 ggacgcccac gtccggggga gtgcgagcct gtccggggtc gagctcgcca ctcccggcgg
     1861 gtacgcgctg ctcgccgacc ggttgcggat cggcggcgag ttctacctac gccggctgcg
     1921 gtgcgcaggc accgtccggc tgcagaacgc cgagatcgga gcaacgctcg actgcacggg
     1981 tgcgcggctg gagcatccgc ggctacggcc cgatggaaca ccgcgaccgt cgttggacgc
     2041 gcgtgcggcg accatcggca aggacatgat ctgcacggag ggcttcacgg cggccgccgg
     2101 cgtgcgagtg cgacgtatgg aggcgcgcaa atcggtgcag ttcgtcgacg cgacgctcgg
     2161 cacgacgtcc acggctggca ttcgatacgc cctcaacgcc tatggcctgg tgaccgcgga
     2221 gcttgtcctg cgtccggccg ccgcccccgc cgggagagtg cgtctggccc aggctcaggt
     2281 gagcaccttc gccgattccg ctccgttatg ggcggccgtt ggtggcatcg atctcgacgg
     2341 ttttgattac cagaagatga atgacacaca tgtgattgac atccagacgc gtcttcactg
     2401 gctcgaacag gtgatgcccg actacgcagc gggaccatac gagcagttgg ccgccgccta
     2461 ccggcgtgcg ggcgacgagg agctgtccga gcgcgtcctc atggcgaggc agagtcggcg
     2521 ctacgccgag gccggcgtcg ccgggcggat ttggggcgcc ctccagcgct ggacggtggg
     2581 attcggctat cagccctggt tagcggtctg ttggctcgtg ctgttcgccg tcctcggtgg
     2641 ggcctggttc gctactcatg cgccgcctcc ggtggacgag ggacaaaacc ccgtcttcaa
     2701 tccgtggctc ttcgccgccg acacgctcct cccgatcgtg aacctcgggc aggacgggta
     2761 ctggcgcctg gacggcgcat cgcaatggat agcgagtgcg ttggtcgcag cggggtggat
     2821 cctcgcgaca acggctgcag cgggcgccgc acgaatactc aagcgggtgt gacgacacgc
     2881 aaagtccacc cgaacgggcg gaaccggggc gcagagctgt aacggtggct cggaacccgt
     2941 ttccacgccc gagtgtcccc gttgtgggtg taagtgcgtc gatcggccag ccagaagagg
     3001 gccgtaggca tcaccatgga aggcgcacag aacgacgacg gaggtgacgc gtgtccccag
     3061 cgacccgcta ctcgacgacc gacgacgact ggaacccggc cacgggtagg catgcgttca
     3121 acgaggacgc ggtgctcacg ccgatcttcc atgcgctcac gcgtggcggg tggcgcagtc
     3181 gccaacacga gccggccgcc ccggcccttc cccccgttcc caccgcgccg ccgcgcgccc
     3241 ccgacccggt cgacgcattc cggcgcgacc cgctgaccgc accgatcccg attcagaccc
     3301 tcgcgcccgc cgccattccc gtcacgccca gctccggcac cggggcccat gccgtgccgg
     3361 cggcaagaca cgagctggtc gaccagggcc ggcaccaccg caggcgcgag gccgtcacct
     3421 cgcactggtg agatcacccg cgctcgaacg cgcgacgaat ggcggccgct ggggagtggc
     3481 cgcgcacgac ggcagtaact gcactactgc ctgcgagggc cggggtacgc gggcaacttc
     3541 ctcggcgccg cgaccccggc cctcagcaac cccccgccgc ccaccccgcg agtcgcccgt
     3601 tcccggcccg cgtgtcgacc ggtttctgcc cgcgtgtcga ccggttccgg tctgtgggag
     3661 agctgtgacg gcagttcggt ctgacagcaa cccctgcgcc tcggacttgt ccacatcgcg
     3721 cctcagttgt ccacagccca gcactgtggc ccctgatcgc actcccgctg cgggatcgtc
     3781 gagccgtgtt tcccaccgct gcgagtactg caggtgcatg cctgcgccgt gatgccgtcc
     3841 ggctactggg cgagaagcgg gttcgcaccg cactcagctc tggcgagttc ggttctccgt
     3901 ttcccggagt gctcgtcgat cctgcgcggg cagcggaacc gtccaccgtc atcgcggcgg
     3961 gttggttggc ctccggaccg gacgcgatca tcaccggttc cactggtgcg ttcctgcatg
     4021 gatacacggc agcagcgccg acgccggtgc acgtcgtcgt gccctacggc agcaggaaac
     4081 ggacgaggcc tgggctggtg gtgcacaacg gccggtcgct cgacttcgat cgacggttcg
     4141 tcgacggcct accggtcctc tgcgtggagc gaatcgtcac cgatctggtg tgctccgctg
     4201 cgccggcgga cgcgctggcc gtggtcgacc aggtattggc tgcgctcgcc ccggctgagc
     4261 gcgcaccatt tcgcgacgag ctccaccgac ggctccggga acgtgcggat ccccgcggaa
     4321 cccggatcgg cacccggcta ctcgatctcg ccaccggtcg agcagactcg ccggccgaga
     4381 gctggctgct ctggcggatc gtcgatctgg gatttcccgt gccgataatc aactatcagg
     4441 tctgcaccat cgatggtgag ccccggtaca gggtcgacct cggctggccc gatctgagga
     4501 tcgccatcga gtactacggc cacgccgccc atgccgagcg tactgaggag gacgaggcca
     4561 ggatcctcga gctcgagcgg cgcggttgga tcgtcatcat cgtccgcgcg gaggatctcg
     4621 cccacatgtc ccgcgtggag cgcgaggtcg acgaggcatt ccgcaggcgg ggcgtgtacc
     4681 tgcgcgagcg caccgtcgga gcgctgcgcg ctaggcggca ccgcgaccta cgtgccagct
     4741 gaaacaccct ggccgactga cctgtcgggg ccgacagtgc cattcgcccg gccctgagca
     4801 gaacctggga cccgggcgcc ggatcgggac gacccgcccg acgggaagag ggcgactcgc
     4861 ggacgcggga tccgggccac tccgttctcg acgccggcgc cgcgccgagc gcgacatcgg
     4921 cgtccgtcgc gtgcttgctc gttgcgcagt tcgaagattc accacccggc acgccgggtg
     4981 taccgagcgg gcggccgcgc cctcatggcg tcgggcaccg ggtggccgcc ccagggcgag
     5041 tagcccgtgt cctcgtgagc cgaattcgac cgagtcgcgg gtgggatccg ggcagctgcg
     5101 ggctggacgt gggcggctcg ccggccgaat ccgggcgact cgcggagagg aacgggcgag
     5161 tcccgggcgg aactgggcga ctcgcgggcg ggaatcgggc gacttccggg cgggaactgg
     5221 gcgactcgcg ggcgggaact gggcgactcg cgggcggatt tcgggcgact cgcgggcccg
     5281 aaacgggcga ctcgcggggg tgggtcaggt gtggaggcgg gctgccactt tccggaggtc
     5341 ggccgttgtg gcggcccggt tcacccgtgt cggctcgcgg tccgccagca cctgctcgcc
     5401 cgccacccag acgtcccgga cgagccggga accgccggcc cagacgaggt tcgacagcag
     5461 ctgcgcgtcg tcctccggcg cgacgaatgc cgagtcgctg aggtcgacgt gcaccaggtc
     5521 ggcccaccgg cccggttcca gggcaccgat atcgtcccgc ccgattgcgg ccgcgccgtc
     5581 ccgggtggcc atgagcagca gctccgcggc cgtgagcgcc gcggcatcgc cgctggtcac
     5641 gcgggcgagc aggccggcga gccgggcctc ggcccacagg tcgaggtcat cgccggaggc
     5701 tggcccgtcg gtgccgagcc cgacgcgcac acccgcgcgc cgcagcgccg acactcgggc
     5761 gataccggcc gcgagcttcg cgttcgagcc cgggcaatgg gcgacggcag tgccacgggc
     5821 agccagcagt gcgatgtcgt cgtcggacag ctgcacgccg tgcgcggcca gcacgcgtcc
     5881 accgagcatc ccgacgcggt cgagcagcgc gggtacggag ccgtacgcct cgcgctgggc
     5941 gaggtcctcg ttggctgcct cggcgacgtg catgtgcacc agcgccccgc gctcacgtgc
     6001 gtgctcgccc accgactcga gcgcctccgg cggcagtgtg taggccccgt gtggtccata
     6061 gccgagctcg atccgctcgc ccggcccgga ccgcagcccg acggcgtcga tccgggccga
     6121 cacctcgtcg cgcatctgct gccacgtgcc gagccggtcc cacccgggcg cggcgatgat
     6181 gcccggcgtc agaacgacgc gcgatccgac ggccgtgacg gcctcgatca ccgcgtccat
     6241 gaagaagtac atctccacgc tggtggtgca gccggtgcgg agcagctcga cggtgcccgc
     6301 ggtcatgccc gcgcgcacgt cggctgcgtc cagcctgccc tccgccggcc acatcacgtc
     6361 gtacaaccag ctcagcagcg gcaggtcgcc ccccatgccg cgcagcaccg acatcggcgt
     6421 gtgggcgtgc gtgttgatga gcccggggac gagggcgccg ggcagcgcgc ggacgggcgc
     6481 gtcagtcggg ggggccgacg accgcggtcc gacgtgggtg atccgcccgc cggcatcgat
     6541 gtcgacgacg gcatcgcgca gaacgctgca gccggggtcg cagggcagta cgaccggggc
     6601 ggtgagccgt accccgaggc tcgtcatcgg cgggcctcca gaagagagcg cagagagcgg
     6661 aacggcggca gccaggcacc ggtgtcgggc agcgagtcga gtgtgatgcg cgggagcggt
     6721 tcgcggaaca ccccctcgat gtcctccaga tcgatgaact cgatcacctc ggacgtgagc
     6781 gcgaagctcg cgtgctcgcg gaagccgatg accgtgacgc tcgtgccctg cttctcgagc
     6841 tcctcgagcg gctcccggaa cgcccgtccg tcaccggacg ccacgacgac gtggcgcagc
     6901 acgccttcct ccgcccggcg gcggatgtgg gcgagcatgt cctcgtcgac atcggagtcg
     6961 tcggtgatct tgggtttcgc gaacacggcg aagcccacgt tccgcagcgc ctcgacccac
     7021 ggccggacga cctccgtgct gcccggcacc acattggtga agacggtggc ctcggcgatc
     7081 gcgtccggtc cggcgagttc cagcaaccac cggcccacgg cgtcgaagcg cggacggaag
     7141 gccgcggtcg gccgcgcgcc gagcagcgag cccaggctca tgtccatgtt gggcgcgtcc
     7201 cagaccagca tcacgcgtgg ggccgggtcc ggggggatgg ccgcggtcac gagcgccgga
     7261 ccggggagca ggtcggggtc atgaggccga ccctagcgct gcgtgaggga tagcatcccc
     7321 ggcgatggac gagagcgggg acgctgaatg cggaagcgtc accacgaccc cgcaggccac
     7381 gatcgcgaac cggtcgacgg gcagggctcc ggggtgacgg accacgaatc cgacgacgtc
     7441 gccgcccgca ccgcgaaggc gatgcaccgg gccgacgctg ccggtcgcgg agcgggggtg
     7501 cggctgctcg atgtcgggcc cggccgcgcc agggtcgcga tgacggtcga gtcgcggcac
     7561 gtcaacggcc acgggatctg ccacggtggg tacctgttcc tgctcgccga cgccgccctc
     7621 gcctacgcat ccaacagcta cggcacctcg gcggtcgcgt cgggcgccga catcgcgttc
     7681 ctacaccccg tgcccctggg cgccgagctc gtggccgagg cggtggaacg ggcgaggacc
     7741 ggacgctcgg ggctctacga cgtgacggtt cggtccggcg agcaggcggt ggcggagttc
     7801 cgtggccgca cccgccaggt gccgggtctg ccgccacccg gctgaggact cgcggcggtg
     7861 ggcggggtca ggagctgcgc ggctggagga aagccgggcg cgtcgaggcg tcgggctcgt
     7921 cgtagtaccg gtggtagacg ggggtcaggc caccggagac gggcggcacg atccagctcc
     7981 agtcggcggg gcagcgacga cccgcactct cctcacgagc gaggtgggtg aggaaccgct
     8041 gggactcgga gtgatggtcg gccatcgtca ccccggccac gtcgaaggag tactgcaccg
     8101 cccgcaccat ctcgacgagt gcgcggtcgc gccacatggt gcgctctgaa ctcatgtcca
     8161 gcccgaggcg gtccgcgact gcggggagca ggttgtagcg gtcggcgtcg acgaggttgc
     8221 gtgcgccgat ctcggtgccg agataccagc cgttgaacgg cgcggccgga taggtcacgc
     8281 cgccgacgac cagcggcatg ttgctgatcg ccggcacggc gtgccaccgc agacgcagct
     8341 cggcgaacca ctcgtggtcg gggtggatga gcgggacctc cagcacggcg tcggccggaa
     8401 cgtcgaacat ctccggctcg ccccctccgt cggagaccag caacggcagc acgtcgaacc
     8461 ggccgggcgg ctcggggcgt tgccacccga gctcgacggc gcggtcggtg aacccggcgt
     8521 accggctgtc gccgcggatc tcgccgctcg tggtgcggtg cccggcgtag cggacgagct
     8581 ggtcgttgtg gatgcgcggt ccgggccggt ccggccggtc ctgggcgaag atcgtgatcg
     8641 tggagcggat ccggccgccg cgggtggcgt cgcgcaggtg ctcgacgcat tccgcggcca
     8701 cgtcggccgg agccgtcacg tgccgcctat cccgcaggcg gaggctgttc cagtacaggc
     8761 ggccgatgca gcgcgccgag ttgcgccagg ccacgcgggc gccgtaggtc agctcgtcga
     8821 cggtgtgggt gtagctaccg gtggagtcga tttcgtgccg cacctgccac atgcgatagt
     8881 cgatcgagcc ggacggcgag gtctcggcgt agaactgggt gaggaactgc tccgcctccc
     8941 cggggtcgac ggcatcgggt gccgcctgcg gtggggcggt gacagccccg gtggccgggc
     9001 aacgctccgc cgagcgggga gcgggtacac caacgttcgt tgcaacgagc gacgtcaccc
     9061 ggcgcctcag cctttcactc gccgacaccc ccggggatcg gggcctgctc ttcctacacg
     9121 gtcacgcgct gtgaccagaa gatggcccgg tgaatgcgcg acggtacaga agatcgcgca
     9181 cgtcacggcc ctcaatacgg gcgcgctgct cgaacttggt gacagggcgc cattccggac
     9241 gcggcgtcca accccccggc tggtccgccg cggcgttctc gaggaacggc tcggcgtcgc
     9301 acacgtcgcg catctggcag gcgtagtcgt cccagtcggt ggccatgtgc aggatgccgc
     9361 ccggcaccag ccgcgaggcc gccagcgaca cgaaatccgg ctggaccagg cgccgcttgt
     9421 ggtgcttgcg cttgggccac gggtcgggga agaagatccg gatgccggcc agcgacgcgg
     9481 caggcacgca ctcatgcagc agtgccacgg cgtcaccgcg cagcagggcg acgttcgaca
     9541 gcccggcgtc ggccatacgc atcagcaact gcgcgaggcc cggctcgaag acctcgaccg
     9601 cgatgtggtc gacatccggg gcggcagcgg ccagtgcagc cgttgactcc cccataccgg
     9661 acccgatctc caggatcagc ggcgcgctcc gtccgaacca ggcgggcggg tcgaacggtt
     9721 ccgcgccgct cagcaggtcg gcgacgtccc ggccacggct cggccaccac cgctcccagg
     9781 cgtgttgctg gccgtccgtg aggcggctgc ggtggtggac gaagctgtgg atcggtgggt
     9841 gcaccgcggt cggccccgcc tcagccaccc acgttcgggt gctccggggc cgcgggagcg
     9901 ccctcccgcg cggtgtcgac gcgcgctccg ctgtcgctct gcgcggcagg cagccgcacg
     9961 cccgcggtgg cgccgggcag tttgcccctc aggaagccgg cgctggtcca ggccagcgcg
    10021 gtgacgaccg cggccgccga cacgacgttc cgctgacgaa gctcccgcag cacggctgtg
    10081 ggcagcacct tcgcgacata gctgcgctcg gtggagagcg cgtcgtcgct gcccacgagc
    10141 gcggagacgg ccgccttcga cagcccctcc gcccagctgc gtcggcgcag gtaggcccac
    10201 tcgacgcggt cctcgctcac gcggtggtcg acgatcgcgc gcggctcgaa cacgatccgg
    10261 atcttctcgc cgacgcgccg gtaggcctgc cgggcgcgga tgcagagctc ggtctcctcg
    10321 cagcccaggg ggttcttacc gatgcggccg atgtcctcgg cgaagccgcc gacgcgctcg
    10381 aacacgtccc ggcggaacga catgttgcag cccatgaggt tgcggacctc ggcctgctcg
    10441 gtgggctggc cggtgtaggt gcagccgacg atccagtcaa gctcgccggt cgcgtcgccg
    10501 tttccggggt cggcaccggg caggacgtgg ggacgtgccg taccggcgcg cactccgcgc
    10561 gccggccagc gcgggtgggc cacccctccc accgcggcga ccgtggggtc gctgtagggc
    10621 gcgagcagcg cggcgagcca tcccgggcgg gccgtggcgt cgtcgtcgag gaacacgacg
    10681 acgtccccgg tcgcctcggc gacggcggtg ttccgggcgc cggagaggcc ctgcttgtgt
    10741 gcgttgggca gcacccgcac cccgcgcgtg gtgaacgcct cctgcgcccg ggacaggagc
    10801 gcggcgttgt ggtcgacgat gaccaaggtc tccgccgcgg tgacgtcctg cgcggcgacc
    10861 gaatccactg cggcaacgat gtcgccccag cgcttctcgg tgtagacgca gatcacgacg
    10921 gtcgccgtga cggcgggcgt actcacgggg cggtctcctt cacgggatgg ggcgaccgcc
    10981 cgtgactagc ggcggtcgga tgtgtgtcgg tcggcacgcc ggtgctcggc cagtagggta
    11041 cgcaggaccc gcgcgccgtc ggcgaatgtg cggaggttcg tctcgccgaa catgcgcagg
    11101 cgctcgatcg agggcacctc ggtgatcttc agaccggcag cggcgattcg gcagttgagc
    11161 accgtctcga tctcaaagcc gtcaccccac agcattccct cagcgggtgg cggcgcgtcc
    11221 accgggggga gctccaggac cggcagcagg tcggcccaga aggcgttgta gccgtagcag
    11281 agatcggtgt acgccgtgcc gaacagcgcg ttcgcgacgc cgttgaggcc ggcgttgccg
    11341 gtcttgcgca ggagggtgat gtcatcgctg ccgccgccgg tggtgaaccg gctgcccttg
    11401 gcgaagtcgg cgcctgcgat caacgccgag acgaacgccg gaatctccgc ggggtcggct
    11461 gagccgtcgg cgtcgaacat cacgatgacg tcgccggtgg ccgcagcgaa tccgcaggcc
    11521 atcgcgttgc ccttgccctt gcgggtctgg gtgatcgtac gcaccgaggg caggacgcgc
    11581 ttggccgcct cgatggagcc gtcgatcgag ttgccatcga ccacgatgat ctcgtggacg
    11641 gcgggacgaa ccgcggcgat cgccggaaga atgatctcca ggttgcgcgc ctcgttgcgg
    11701 gttggcacca caacggagac ggccgggaac cgggtgcggc tcggattggg acgtggtcga
    11761 gggcgacgcg atgctcgacg ggccgcctcg tggtgtggcg cgtggtgcgc cgcctccgcg
    11821 gggacagctg ggcgggcagg cgaaacggcg ctcacacaac ctccagacaa gtccgctcga
    11881 ctgggacaac gagaccgtag cgagcgggtt gcggtaacta gctctgttcc ccccgaatgg
    11941 gggtaggtga tgaatctgtg accggaagcg ggtagggata cggaccgcac cgggagcggt
    12001 cgcgcgccat cctcttccgc atgagcaccg agaccgtgga tccccatgtc gctctcgccg
    12061 ccgagctggc tcggctcggc cggaggctcg acgagatggg cgcggagttg ctgcggctgc
    12121 atacagacgc cgccccggtc gctgccggac cggcgcccga cgcagggacg gcaccgtggt
    12181 cacgatcagg agacgcacgt ccaggcgcgc aggttgcctc aaatgtcgat tccgccgctc
    12241 gttcggctct ggatgcgccg ggcggcatcg ccgccgggca ggggacaccg gcgccctact
    12301 ggacgcccgt cccgcagccg tcggggccgg ccgaggcccg cccgtacccg ctggtcacgc
    12361 gacgcttgac gtggacggcg atctccggag cgaggctcct cgcctggacc ggcggcggag
    12421 tgacgttgct cggcgtcgtc ctgctgctcg tcctggccgc ctctcgaggc tggttctccc
    12481 ctccggctcg ggtgacggtg ggcgcgctgc tcggtgtcgc gctgatcggc ctcgcgttgt
    12541 ggctgcaccg tcgggagagt gcccgggcgg gcgctctcgc cctggctgcg acgggattcg
    12601 cgacgctcta cctcgtggtg gcggcggcca cggcgctgta cgactatctc cccgacgtgc
    12661 cggcgctgct ggtcgcgctg ctggtggcgg cggccgggct cggcctggcc gatcggtggc
    12721 gggcgcagct gctgggcggc ggcgtggtcg tgggggcggc gctgctggcg cccgtgatcg
    12781 tcacggactg gctgctggtg gcgctcgcgc tggcgctgca gctggcggcg ctgccggtcg
    12841 tgctgcgcag ggggtggtcc gtgctgatgc tggtcgcggc ggccggaccg gtgatcttcg
    12901 gatccgccgt cggcgccatg gtggtcgtgg acggagacgt ggtgacgatc gccgtcgtgc
    12961 tcggtgtgct ggccgtgggc ctcgggaccg cgatgcccgc cgcgcggatg ctgccgcgag
    13021 agccggtggc ggggctggtc gccgccacgc ccgtccccgt gctggcgacg ggggccgccc
    13081 tcggcggctg ggacggcggc gcgctcacgg cggtcgccgc gctggccatg ggtgggtttg
    13141 ccgcggtacc gggcctggac cgcctgctcc gcaccgtggg cgttaccgcc gcggccgtcg
    13201 cgctgttcca ggcgacgctg gtcgccgtgg aggggtccac cgcaactgca gtgctgatcg
    13261 gccaggcgat cgtggcggcc gtggtggctg ccgtgctgcg ctcgcggctg ccgatgatga
    13321 tcgcgttggc ctacggcacc gtcggggtac tgatggggct ggctcaggac gccccggtcg
    13381 ctgcgctcgt gcagttcccg gcgatcccct acgaccagcc cggcccgggc gctctcctca
    13441 ccggcgtcgg cgtctcggcg ctcgccctgg tactcgccgt ggcgctgctc gtggcgggcg
    13501 gcagggtcga gctgatccgg ccggacacgg cgtccgcccc gctgtggata ccgatcgggc
    13561 tcgtcgggct ctatggcgcg acctcgttga tggtcaccat cgcgctgctc gtctcgacgg
    13621 accgcgccgg cttcaccggc gggcacgccg tggtgacggt gtcctggacg atcgccgcgc
    13681 tcgtcctgct ggcccgcggg atcagcaggg ccgcgctgcg gatcaccggg ctcgtgctgg
    13741 tcgccgccgc cgtcgcgaag ctggtgctgt tcgacctcgt cgcgctcgat ggagtggcca
    13801 gggtggcggc gttcctcggt gccgggctgg tgctgctggc ggcgggcacc cgctacgcac
    13861 ggctcgtcgc ggaggccgag gcggaggcga gcatggagcg cgaggcgcag ggcgcccccg
    13921 gaaacgcgag ctgagcctcc cgttcgcgat cgcacaccgc tacggccgtt cggccccgcc
    13981 gaccgtccgg cgctgcgacg ctccgggcat gcctgatcgg tacatcgacg ccgtccgcgc
    14041 ccgccgcgtc gagctgcgcg cgtggcgggg cctgctgacc ggcggtcacc gggcggagct
    14101 gcgcagctgc ctggccctga gccggatcgc cgcggcagcc gaggcgagcg gggcgctggc
    14161 cgggctcgcc cgggaggcgg ccgaccacat cgagaccggc gaccgcgcgg cccgggggca
    14221 g
//
LOCUS       DASRSC010000240         6309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3205028,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000240 DASRSC010000000
VERSION     DASRSC010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6309
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3205028"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFE39_11675"
     CDS             <1..496
                     /locus_tag="VFE39_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005440906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HET6259300.1"
                     /translation="PGATPAIRFNFLSTEQDRREWIESVRIAREILGQPALAQYDGGE
                     AAPGPAVQTDEQVLAWVAGHAVSAMHYAGTCRMGTGGDAVVDPTSMRVHGTDGLRVVD
                     ASIMPNVTNANTFAPVVMIAEKAADMILGRTPLPAQLEPYHQHRNTQAQPAQPTEHSA
                     QPIS"
     gene            526..2166
                     /gene="menD"
                     /locus_tag="VFE39_11680"
     CDS             526..2166
                     /gene="menD"
                     /locus_tag="VFE39_11680"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HET6259301.1"
                     /translation="MNPSTMQAEVIVDELVRCGATDAVLCAGSRNAPLSFALQAADAA
                     GRIRLHVRIDERTAGFLALGLALRSGRPVPVCTTSGTAVANLHPAVLEASYAGVPLLV
                     LSADRPQEMVGTGANQTIDQAGLFGGAVRLALTGALGGGAEGNGGWRSLVDRAVAAAT
                     GALGGPPGPVQLNLPFAEPLVPDGSGVAPAGRDGGGPWTSVPPIAREVPVLPLDPAAP
                     TLVVAGSGAPAGLHDLPVPVIAEPASQPWPTAVRTGPWLLGTPSAGLLKPAQVVVAGR
                     PTLHRSVQRLLADPSVATYALADPDGRPWTDVAGTVRAVGALPTLTPDPSWIHRWRTA
                     DTAAAKALDQALDAETAPVGLRLARAVLAALPDGAQLLLGSSNPVRDVSLAAAPRHGL
                     TVLANRGVAGIDGSVSTAMGAALAHGGPAYALLGDLTLLHDATGFVIGPDEPRPDLTV
                     VVLNDGGGGIFGLLEQGAPEHAAAFERIFGTPHNVDLAALCAATGVAHVRVDVAGIPA
                     ALAPAPGVRVVEVPADRLSLRAGHAALRATVDAAVAAA"
     gene            complement(2205..3443)
                     /locus_tag="VFE39_11685"
     CDS             complement(2205..3443)
                     /locus_tag="VFE39_11685"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HET6259302.1"
                     /translation="MTIAPLPTGLGVRTRLIDDPGRLIDLLPDDRNPLSWVRGPDGLV
                     GWGEVARFTGTGPGRFAEADAWWRSFTDRLHVTDDVGMPGTGPVAFASFTFTDTSPGS
                     ALVVPRVLVGRRDGRSWITEFSRGNGPSSVRAVSPVRASGTLRFADGMLPVAGYRAAV
                     TEAVRRMRAGELEKACLAHDLLAVGEAPLDPRFLLGGLAERYPTCWSYAVDGLVGATP
                     ELLVRRAGGVVESRVLAGTIWPGEGDDLSGQLLGSAKDRHEHALAVDSLAAALRPLCS
                     TLDVPQTPGVIALHNVSHLASDVRGTLHPAAPASLLELAEAVHPTAAVGGTPRDTALV
                     LIAELEGMDRGRYAGPVGWVDGHGDGEFGIALRCAQLDGPVARLFAGCGVVADSDPDR
                     EAREAAAKMVAVRDALEAGA"
     gene            3537..4022
                     /locus_tag="VFE39_11690"
     CDS             3537..4022
                     /locus_tag="VFE39_11690"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HET6259303.1"
                     /translation="MTTGPGRTDTRAVDELAALVRPAARGVAARLPEIIAVVAVLVTV
                     LAAFAVAGAALDDRAIAANPAVAEAEVLRGWSFSRTLVRFTVANGEAVVPEHGVFYPR
                     GLQEGERVAVEYDVTKPDLVRVAGRSALDGTVPLLLGVLGTWAVLGPLAVWLRRRRAG
                     T"
     gene            complement(4026..4895)
                     /locus_tag="VFE39_11695"
     CDS             complement(4026..4895)
                     /locus_tag="VFE39_11695"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6259304.1"
                     /translation="MRATPFAFVIIWASGFVVAKYAAPYAEPLSFLILRYAGVVVLML
                     ALALVAGAAWPGRRQALHIAVAGVGIQAGYLGGVWAAVAAGMPAGVTALVVNLQPVLT
                     AAFAVLLGERLGRLQVFGLLLGFAGVALVVSSKLTTAGITPLTLGLTTMALFAITAGT
                     LYQKRFCPEFDLRTGQVVQFAAAILVTLPFAVTFESFRFDWTPQLVGALAWSVLVLTG
                     GGISLLFLMLRRGAAAQVTSYFYLVPGITALMALVMFHETLGPIAIAGMVVAVLGVAL
                     ATRANGRARSDSP"
     gene            complement(5065..5895)
                     /locus_tag="VFE39_11700"
     CDS             complement(5065..5895)
                     /locus_tag="VFE39_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HET6259305.1"
                     /translation="MTVQQHPSAPPSPLPVERGLLTRALEVAGRLANDAAEVITATAG
                     RGHQPGATHKANPFDWVTDTDRTLERHTRRVLASEFPGVPVVGEEFGADADAANAPYR
                     WVVDPVDGTANYVAGFPWYAYSLALVDAFGPVVGVIADPSRAQIYAAARGRGVRANGV
                     AVTVTPRSPAGGLVCAELSLERAPMFARQAAAAHAGVRVLGSSALAITQVALGHAVAA
                     VLDGYQEWDVAGAACLAAEAGALVVDGNGQPDPLPTGAMVVAVPGALDEVLRWWHADR
                     "
     gene            6050..>6309
                     /locus_tag="VFE39_11705"
     CDS             6050..>6309
                     /locus_tag="VFE39_11705"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6259306.1"
                     /translation="MRVAIVSECFLPVINGVTNSVLRVVEHLTAAGHETLVIAPGMDG
                     PTEYAGARVVRIAAVDLPVVSSMPVGVPSRRVLTALREFTPDV"
BASE COUNT          855 a         2371 c         2282 g          801 t
ORIGIN      
        1 cccgggagca acgcccgcca tccggttcaa cttcctgtcc acggagcagg accgtcggga
       61 gtggatcgag tccgtccgga tcgccaggga gatcctcggc cagcccgccc tcgcgcagta
      121 cgacggcggc gaggcagccc ccggaccggc ggtgcagacc gacgagcagg tgctcgcctg
      181 ggtcgccggc cacgcggtct cggcgatgca ctacgcgggc acctgccgga tgggcaccgg
      241 gggcgacgcc gtcgtcgacc ccacgtcgat gcgcgtccac ggcaccgacg gcctgcgcgt
      301 ggtcgacgcg tcgatcatgc cgaacgtcac caacgccaac accttcgccc cggtcgtgat
      361 gatcgccgag aaggccgccg acatgatcct cggacggacc ccgctgccgg cgcagctcga
      421 gccataccac cagcaccgga acacgcaggc acagccggcc cagcccacgg agcattcggc
      481 acagcccatc agctagggcg gcgcggcggc gcctacgctc gagccgtgaa cccgtcgacg
      541 atgcaggccg aggtgatcgt cgacgagttg gtgcgctgcg gcgccacgga cgcggtcctg
      601 tgcgcgggct cgcgcaacgc tccgctgtcg ttcgcgctgc aggccgccga cgctgccgga
      661 cgaatccggt tgcacgtccg gatcgacgaa cggaccgcgg ggttcctggc gctcggtctc
      721 gccctgcggt ccggccggcc ggtgccggtg tgcaccacgt cgggcacggc ggtggccaac
      781 ctgcaccctg cggtgctcga ggcgtcctac gccggggtgc cgttgctcgt gctcagcgcc
      841 gaccggcccc aggagatggt cggcaccggg gccaaccaga cgatcgacca ggcggggctc
      901 ttcggtgggg ccgtgcggct cgcgctcacc ggggccctcg gtggcggcgc ggaaggcaac
      961 ggcggctggc gttcactagt cgaccgtgcc gtcgccgcgg cgacgggagc cctcggcggg
     1021 ccgcccggac cggtccagct caacctgccc ttcgccgagc cgctggtgcc cgacggatcc
     1081 ggtgtcgcgc cggcgggccg cgacggaggt ggaccgtgga catcggtacc accgatcgca
     1141 cgtgaggtgc cggtgctgcc gctcgacccc gccgcgccga cgctcgtcgt cgccgggagc
     1201 ggtgccccgg ccggcctgca cgacctaccg gtgccggtga tcgccgagcc ggcgtcgcaa
     1261 ccgtggccca cggccgtgcg caccggaccg tggctgctcg gcaccccgtc cgcgggacta
     1321 ctcaagccgg cgcaggtggt ggtggccggc cgacccacgc tgcaccggtc cgtccagcga
     1381 ctgctcgccg acccgtccgt cgccacctac gcgctcgccg acccggacgg ccggccgtgg
     1441 accgacgtgg caggcaccgt ccgagcagtc ggtgcgctgc cgaccctgac ccccgacccg
     1501 tcgtggatcc accgctggcg gaccgccgac accgccgcgg cgaaggcgct ggaccaggcg
     1561 ctggacgcgg agaccgcgcc cgtcggcctc cgtctcgcgc gggccgtact cgcggccctg
     1621 ccggacggcg cccagctgct gctcgggtca tcgaaccccg tgcgggacgt ctcgctcgcc
     1681 gctgccccac ggcacgggct caccgtgctg gccaaccgtg gtgtcgccgg catcgacggc
     1741 agcgtgtcga ccgcgatggg tgctgcgctc gcccacggcg gcccggcata cgcgctgctc
     1801 ggtgacctca cgctgctgca cgacgccacc gggttcgtca tcggcccgga cgagccacgg
     1861 cccgacctga ccgtcgtcgt cctcaacgac gggggcggcg gcatcttcgg gctgctggag
     1921 cagggcgcgc ccgagcatgc ggcggcgttc gagcggatct tcgggacgcc gcacaacgtc
     1981 gacctggccg ccctgtgcgc tgccaccggt gtcgcccacg tccgggtgga cgtcgccggc
     2041 atcccggccg cactcgcccc cgcccccggc gtgcgggtgg tggaggtgcc cgccgaccgc
     2101 ctgtcgctgc gtgcggggca cgcggcactg cgggccaccg tggacgcggc ggttgccgcc
     2161 gcctgaacga cggccccgga caccccgatg cggcgagccg gtgctcaggc tccggcttcg
     2221 agcgcgtccc ggacggccac catcttcgcg gcggcctccc gcgcctccct gtcgggatcg
     2281 gagtcggcca cgacaccaca ccccgcgaag agccgcgcga ccggaccgtc cagctgcgcg
     2341 cagcgcaatg cgatgccgaa ctcgccgtcg ccgtgcccgt cgacccagcc gacagggccg
     2401 gcgtagcggc cgcggtccat gccctcgagc tccgcgatca ggacgagcgc ggtatcgcgg
     2461 ggcgtgcccc cgacggcggc cgtcgggtgc acggcctcgg ccagctccag cagcgaggcg
     2521 ggagcggcag ggtggagcgt gccgcgcacg tcgctggcga ggtgggacac gttgtgcagc
     2581 gcgatcaccc ctggcgtctg cggcacgtcg agcgtgctgc agagggggcg gagcgcagcg
     2641 gccaacgagt cgacggccag cgcgtgttca tgccggtcct tggcggagcc cagcagctgc
     2701 cccgacaggt cgtcgccctc ccccggccag atcgtcccgg ccagcacccg cgactcgacg
     2761 acgcccccgg cgcgccgcac cagcagctcg ggcgtggccc ccaccagccc gtccacggcg
     2821 tacgaccagc aggtcgggta gcgctcggcc agcccgccga ggaggaaccg cgggtcgagc
     2881 ggggcctcgc cgacggccag caggtcgtgg gcgaggcagg ccttctcgag ctcgcccgcg
     2941 cgcatccggc gcaccgcttc ggtcaccgcc gcgcggtaac cggcgacggg cagcatgccg
     3001 tcggcgaagc gcagcgtgcc gctggcccgg accgggctga ccgcccgcac cgacgacggc
     3061 ccgttcccgc gcgagaactc agtgatccag gaccggccgt cgcggcgacc caccagcacg
     3121 cggggcacga cgagcgccga gccgggggac gtgtcggtga acgtgaagct ggcgaacgcc
     3181 accggaccgg ttccgggcat ccccacgtcg tccgtcacat gcaggcggtc ggtgaacgac
     3241 cgccaccagg cgtccgcctc ggcgaaccgc cccggtccgg tgccggtgaa gcgggcgacc
     3301 tccccccagc cgacaagacc gtcaggtcca cgcacccagg acaacggatt gcggtcgtcg
     3361 ggtaggaggt cgatcagccg gcccgggtcg tcgatgagcc tggttcgcac gcccaacccg
     3421 gtcggtagcg gggcgatcgt cacgaaatcg agagtaggag cctgcgccgg gccgtgcgcg
     3481 cccggcccgg gaggtggtgc cgacactcgg gtgtccacca ggcataggat cgacttgtga
     3541 cgactggtcc cggccgaacc gacacccggg ccgtcgacga gctggcggcg ctcgtgcgcc
     3601 cggccgcgcg gggggtcgca gcgcgtctgc cggagatcat cgcggtagtg gcggtgctgg
     3661 tcaccgtgct ggccgcgttc gctgtcgcgg gcgccgcatt ggacgaccgc gcgatcgccg
     3721 ccaacccggc cgtggccgag gccgaggtgc tgcgcgggtg gtcgttctcc cgcacgctcg
     3781 tccgtttcac cgttgcgaac ggcgaggcgg tcgtgccgga gcacggggtc ttctacccgc
     3841 ggggcctgca ggagggcgag cgggtcgcgg tcgagtacga cgtcaccaag cccgatctcg
     3901 tacgtgtggc cgggcgctcc gcgctcgacg ggacggtgcc gttgctcctc ggcgtgctcg
     3961 ggacgtgggc ggtgctgggg ccgctcgctg tctggctgcg ccggcgccgc gccggcacct
     4021 gacgctcacg gcgaatcgga tcgagccctg ccgttcgcgc gggtggccaa ggccaccccg
     4081 agcaccgcca ccaccatccc ggcgatggcg atcggtccga gcgtctcgtg gaacatcacc
     4141 agcgccatca gcgcggtgat cccggggacg aggtaaaagt agctggtcac ctgggcagcc
     4201 gcgccgcggc gcagcatcag gaacagcagc gagatcccgc cgccggtgag caccagtacc
     4261 gaccaggcca acgcgccgac cagctgcggg gtccagtcga accggaagga ctcgaacgtc
     4321 accgcgaacg gcagcgtcac caggatcgcc gccgcgaact gcacgacctg tccggtgcgc
     4381 aggtcgaact ccgggcagaa ccgcttctgg tacagggtgc ccgcggtgat cgcgaacagc
     4441 gccatcgtgg tcagcccgag cgtcagcggg gtgatcccgg cggtcgtgag tttgctcgag
     4501 acgacgaggg cgaccccggc gaacccgagc agcagcccga agacctggag ccgcccgagt
     4561 cgctcgccga ggagcacggc gaacgcggcg gtcagtaccg gctgcaggtt caccaccagc
     4621 gcggtgaccc cggccggcat gcccgccgcg acagccgccc acaccccgcc gagatagccc
     4681 gcctggatgc ccacccctgc caccgcgatg tgcagtgcct gccgtcgccc cggccacgcg
     4741 gctccggcga cgagcgcgag cgcgagcatc agcacgacca ccccggcgta gcggaggatc
     4801 aagaagctca gcggctccgc gtacggggcg gcgtacttcg cgacgacgaa gcccgacgcc
     4861 cagatgatca cgaaggcgaa tggggtggcc cgcacggcca gcgtggcggc ggacgaggtg
     4921 gacgtcgtgg cgatgcccga ccctaacccg cgagccgccc ggattcggcc cgtgagtcgc
     4981 ccggtctggt tgcgcgagtc gcccggattc ggcccgtgag tcgcccagcc cgatcgcgcg
     5041 agtcgcccga tccggtctga ggcgctaccg gtccgcgtgc caccacctca gcacctcgtc
     5101 gagcgccccc ggcacggcga ccaccatcgc accggtgggc agcgggtcag gctgcccgtt
     5161 cccgtcgacg acgagcgcgc cggcctccgc cgcgaggcag gccgcacccg cgacgtccca
     5221 ttcctggtag ccgtccagca ccgccgcgac ggcatgcccc agcgccacct gcgtgatcgc
     5281 gagagccgac gacccgagga cccgcacccc ggcgtgcgca gcggcggcct gtcgggcgaa
     5341 catcggcgcg cgttccagcg agagctcggc acacaccaga cctccggcgg gtgagcgtgg
     5401 cgtgaccgtc accgcaacgc cgttcgcccg caccccacgt ccgcgggcgg cggcgtagat
     5461 ctgagcccgg ctggggtcgg cgatcacccc gacgaccggt ccgaaagcgt ccaccagggc
     5521 gaggctgtag gcgtaccacg ggaagccggc aacgtagttg gccgttccgt cgaccggatc
     5581 gaccacccac cggtagggcg cgttggcggc gtccgcgtcg gccccgaact cctcgccgac
     5641 caccggtacc ccggggaact ccgatgcgag tacccgacgc gtgtggcgct ccagggtgcg
     5701 gtcggtgtcg gtgacccagt cgaacgggtt cgccttgtgg gtggcgccgg gctggtggcc
     5761 acggccggcg gtagccgtga tgacctctgc cgcgtcgttg gcgaggcggc cggccacctc
     5821 cagggccctg gtgagcagcc ctcgctccac cgggagcggt gacgggggcg cgctcgggtg
     5881 ctgctggacg gtcatgtggt ccgagcgtca ccgcctccgg tgtccccggt gcgtcgccca
     5941 ggtgacgtgt catggatcac ccggccaatt gtgtgcctga catgggttgt tggccgcgag
     6001 ttcgatggcc cgtcaacgtc tcgcccttcg ggcgcgcgac ggtgcccggg tgcgcgtcgc
     6061 catcgtctcc gagtgcttcc tccctgtgat caacggtgtc acgaactccg tactccgggt
     6121 cgtcgagcac ctgacggcgg cggggcacga gacgttggtg atcgcgcccg ggatggatgg
     6181 tcctaccgag tacgccggtg cccgggtcgt ccggatagcg gccgtggacc tgccggtcgt
     6241 ctcgtcgatg ccggtgggcg tgccgagccg ccgcgtgctg accgccctgc gcgagttcac
     6301 ccccgatgt
//
LOCUS       DASRSC010000241        11726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4187639,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000241 DASRSC010000000
VERSION     DASRSC010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11726
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4187639"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /gene="upp"
                     /locus_tag="VFE39_11710"
     CDS             <1..613
                     /gene="upp"
                     /locus_tag="VFE39_11710"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET6259307.1"
                     /translation="VVDHPLAKARLSELRDARTDNPTFRAALRDLTLMLIYEATRDAP
                     LVTAPLHTPVARTLGHRLANPPLLVPVLRAGLGMADAALGLMPEAQMGFVGLSRDEET
                     HQPVPYMESLPETLDGRPVFVLDPMLATGGSMAHTIRLLTERGATDVTSICVLAAPEG
                     VEHLRASGLPVRVVTASIDERLNDSAFIVPGLGDAGDRQFGAV"
     gene            complement(849..1418)
                     /locus_tag="VFE39_11715"
     CDS             complement(849..1418)
                     /locus_tag="VFE39_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WHG domain-containing protein"
                     /protein_id="HET6259308.1"
                     /translation="MVRPKVHDEALRGRLLGRALERVAADGVSGLSLRALAADCGTST
                     TAVYSLFGGKTGLLAALFDEALRLFREHLAVTPGDDSLDDLVRLGLAYQRGALAQPHL
                     FEVVFDGGPPNRAAGAALAPLREFVERAVKDDALRPDIDPTTAALALWATVHGWVSLQ
                     LRGLLPPGTEARFEDVLRCVTAGWTATAP"
     gene            complement(1478..3088)
                     /locus_tag="VFE39_11720"
     CDS             complement(1478..3088)
                     /locus_tag="VFE39_11720"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET6259309.1"
                     /translation="MRWIADDPDPETRMELQRVLAAAMVDAPGAVDDLHDRMAGMLRF
                     GTAGLRGPVRAGPAGMNVAVVRRATAGLAAWLARGGERGSVVIGRDARHGSEAFATAA
                     AEVLAAAGFRVLALPAPLPTPLLAFAVRELGAVAGVQITASHNPPADNGYKVYLADGA
                     QLAPPSDAEIEAAINATPAAVSIPTSGEFSTVDVAEAYLDRAGQLPRGPARQLRVALT
                     PLHGVGGATAVHALHRAGFTDVHVVAAQAMPDPDFPTVSFPNPEEPGATDALLALATE
                     VGADLAIALDPDADRCALAVPVAGFGEGEGGSAWRLLTGDESGVLLGDHLLRSGDTHG
                     VPYRDPLVATTVVSSSMLRPLATAYGARYAETLTGFKWIVRGGAGLVYGYEEALGYCV
                     DPDAVRDKDGIAAAVLACDLAAGLKAAGRSLTDRLDELAVAHGVHRTEGLSLRRDPAE
                     RDAVVERLRSGPPTGWSAEYPAPDVLVLRRDGERLVIRPSGTEPKLKAYLEVVEPVPD
                     AAALAAAKAAAQGRLDALRVEACDLLES"
     gene            complement(3118..3918)
                     /locus_tag="VFE39_11725"
     CDS             complement(3118..3918)
                     /locus_tag="VFE39_11725"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET6259310.1"
                     /translation="MSVDASAPPAVAAAAAIAAATGVDAHDVAVVLGSGWRPAADVIG
                     EPAHEIVMADLPGFVPPAVAGHGGTIRSVPVGGHRALVLLGRTHLYEGHGVDPVAHGV
                     RTAAAAGCRTVVLTNAAGGIREGMRVGEPVLISDHLNLTGRSSLHGPRFTDLTDLYSA
                     RLRALAREIDPTLSEGVYAGLPGPHFETPAEIRMLRRLGADLVGMSTVLEAIAARAEG
                     VEVFGLSLVTNLAAGMTGEPLDHHEVLAAGAAAAGRMGELLRDLIARS"
     gene            complement(3956..5602)
                     /locus_tag="VFE39_11730"
     CDS             complement(3956..5602)
                     /locus_tag="VFE39_11730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6259311.1"
                     /translation="MPQDSPEDQHVRRIGGRYRLSDLLGSGAMGTVWAGYDEVLQRRV
                     AVKELKVPPGVPEQEAAEARERIMREARALGGLSHPNIITVFDVVDVDGAPMVVLELV
                     PSRNLATMIGEQGTLTLQQTAVVGFATAGGLRAAHRSGITHRDVKPGNVLVADDGRVK
                     LTDFGIARNIADAPMTSVGLVLGSPAYIAPEVAAGQPVTPAADLWGLGATLFAALEGR
                     PPYDVHGDPVSTITEVVDGEVPRPRTSSPVADVIMALMVKDPDARMPLEEVRHRLRPL
                     IDDPDDPLYPGSPDAPTIGSGLAVPSSAARFEFAGGRPPPLPPPPTGARGPAETAAPL
                     ATSPGPLPPVRNRPAAAPRAASTAAPARHSVSSESVSGWSAAGLVVAGAAVVLAGALG
                     GWSVTRLVGGQSPLTTVNVTSTSTAMLEHTDALGFSIPVPQDWTEQRNEPAGGLPSVD
                     FVSSARTEELAVQRADSTEAAQAVAGTLLDPPAPAPAPAPADAVQLRYESGDYTSWRR
                     ILPADKGVWTITLTVPRVAAGSTSAALFELVAGGFTSTPA"
     gene            6015..7253
                     /locus_tag="VFE39_11735"
     CDS             6015..7253
                     /locus_tag="VFE39_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET6259312.1"
                     /translation="MTLLEAHTDLVPPAEAAVTDFGAGSGPAWLDSWLLAHRSDVVAW
                     RRALHALPELGRVEHRTTELLARRLMAAGLEPRTLPGGTGLVCDIGYGDRCVALRADL
                     DALPLQEDTGLPYASTVDGVMHACGHDAHAAMLLGAGLALASAPALPGRVRLIFQPAE
                     EVQPGGAIDMVADGVMEGVQRVFALHCDPRLEVGKVGTRVGPITSACDVLEVRLTSPG
                     GHTSRPHLTADLVEALGILITQLPLLLTRQVDPRSGTVLVWGAVQAGEAANAIPQHGV
                     LRGTLRTADHHTWGELEGKVRALVAALLAPTGTGYVLEHIRGVPPVVNEAVSAGMLAD
                     AATAVLGPDAATSTEQSSGGEDFAWYLEHAPGAMARLGVWPGHGPMRDIHQPTFDLDE
                     RALPFGVRMHVQVALAALTT"
     gene            complement(7556..8005)
                     /locus_tag="VFE39_11740"
     CDS             complement(7556..8005)
                     /locus_tag="VFE39_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HET6259313.1"
                     /translation="MPLYAAYGSNMDPAQMKERAPHSPMAGTGWLVDWRLTFGGEDYG
                     WEGALSTVVEEPGSQVFVVLYDVPDHDSAQLDRWEGGELGLHKKLRLRVHTLEGDVLA
                     WIYVLNDYEGGEPSARYLGVIADAAEQAGAPDDYVSALRSRPSRKSV"
     gene            8078..9481
                     /locus_tag="VFE39_11745"
     CDS             8078..9481
                     /locus_tag="VFE39_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone dehydrogenase"
                     /protein_id="HET6259314.1"
                     /translation="MTRIVIMGGGPAGYEAALVAAQHGSDVTVVEREGMGGACVLDDC
                     VPSKTLISSAGIRVDLHRAPDLGLIADRQAIGVDLPTVHARIKRLALAQSADVRARVE
                     REGVRIVRGTGVFTDAPSVRAPHVVETRTLDGGREELPADVVLIATGATPRVLDDAKP
                     DGERILTWRQLYDLEALPEHLIVVGSGVTGAEFVSAYVELGARVTLVSSRDRVLPGED
                     ADAAAVLQDVFAERGVEILSQARADSVRRDGDGVVVTLADGRTVTGSHALMTVGSVPN
                     TADLGLEKIGVETGRGGFVPVDRVSRTSVPGVYAAGDCTGVLMLASVAAMQGRIAMWH
                     ALGEGVAPIKLKTVAAAVFTRPEIATVGISQNAIDAGEVPARTIMMPLSTNPRAKMQG
                     LRRGFVKLFCRPATGVVVGGVVVAPVASELILPIALAVQNGLTVADLAHTFSVYPSLS
                     GSVTEAGRQLMRHGDLD"
     gene            complement(9491..9766)
                     /locus_tag="VFE39_11750"
     CDS             complement(9491..9766)
                     /locus_tag="VFE39_11750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HET6259315.1"
                     /translation="MARGVDRTAAAAAAVLVGAAVATELRKPPVERTWHGSVAGLVPY
                     DLRPPTFDRLRSRLWNPDDPAVLVPHPFGVGWTVNFAALRELASGRA"
     gene            9884..10243
                     /locus_tag="VFE39_11755"
     CDS             9884..10243
                     /locus_tag="VFE39_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259316.1"
                     /translation="MTTVPATSRVPAGAVLLAGLGAQLVDDAVFLVLPPTALLAPLSG
                     LLAILLTAGAARIVTRDRTGAVVARTGLAVGLTSAVIGLLVGGLGMVAILLAGVTVVA
                     GVAGAVAGRGLTKNDIS"
     gene            complement(10436..11227)
                     /locus_tag="VFE39_11760"
     CDS             complement(10436..11227)
                     /locus_tag="VFE39_11760"
                     /inference="COORDINATES: protein motif:HMM:NF025377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESX secretion-associated protein EspG"
                     /protein_id="HET6259317.1"
                     /translation="MTEPPLVLDPPHMHRHVLTAVEFDVLWERLGLGATPVVLRLASP
                     GRTHAERRDVLAAGWQALRERGLAGPAGADPELVRLLHLLARPAEQLELRGWWDRSVR
                     AVAAGARGACVLAVRQDDTVTLGSHRSLPSALLRALPQAGPGPGRASTVPAAVLAAAT
                     AAQGAGLRSALLAKHVPSVDAGLLARMLIGIEGRAQIVALVSDRWGVLRRSGGVLAVL
                     DGPRGRYLMTRTVGEDGVEWATVAPTDARRLRHLVAELLAPTSQT"
     gene            complement(11393..>11726)
                     /locus_tag="VFE39_11765"
     CDS             complement(11393..>11726)
                     /locus_tag="VFE39_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259318.1"
                     /translation="PGATLHPVALQSWRRRAEDWLSTRRASRPPALAMTAFAQWIDQL
                     PGRPVFVADTAEPDYLFLYWYLQRFTGRWPFASTLTDASLHGRMSCSTLCPLAGCRMP
                     EPSLARTS"
BASE COUNT         1535 a         4311 c         4338 g         1542 t
ORIGIN      
        1 ggtcgtcgac cacccactgg ccaaggcccg gctctcggag ctgcgcgacg cgcgcacgga
       61 caaccccacg ttccgcgcgg ctctgcgcga cctcaccctg atgctgatct acgaggccac
      121 ccgggacgcg ccgctggtca ccgccccgtt gcacacgccg gtggcgcgca ctctcgggca
      181 ccggctggcg aaccccccgc tgctggtgcc ggtgctgcgc gccgggctcg gcatggccga
      241 cgccgcgctg gggctgatgc ccgaggcgca gatggggttc gtcgggctct cccgcgacga
      301 ggagacccac cagcccgtgc cctacatgga gtcgctccct gaaacgctcg acgggcgccc
      361 tgtgttcgta ctcgacccga tgctcgccac cggaggctcg atggcacaca ccatccggct
      421 gctcaccgag cgcggggcca ccgacgtgac gtcgatctgc gtgctggccg cccccgaggg
      481 tgtcgagcac ctgcgggcca gcgggctacc ggttcgcgtc gtcaccgcca gcatcgacga
      541 gcggctcaac gactccgcgt tcatcgtccc cggcctcggc gatgctggcg accgccagtt
      601 cggcgccgtc tgacgcgaca cgccgcagga ccgtcaccat tcctggtgaa tctcgcgccg
      661 cggcgagacg ctcggccccg ccgcatcgac ctacgggacg acggcgggcc gaggggcggt
      721 actcggacga gggggcggac gacgattgcc gagcggcagc cggtggtcgt cgaacgctgt
      781 ggtccgcgcc cggcccgccg tcccacagtg agtgctccgg ctgcggtcgt ccgccgcgca
      841 cgtccgtcct agggcgccgt cgcggtccag ccggccgtga cgcaacgcag gacgtcctcg
      901 aagcgggcct cggtgcccgg cgggagcaga ccccgcagct gcagcgacac ccagccgtgc
      961 acggtggccc agagagcgag agcggcggtg gtggggtcga tgtcgggccg cagtgcgtcg
     1021 tccttgaccg cgcgctcgac gaactcgcgc agcggcgcga gcgcggctcc ggcggcgcgg
     1081 ttcgggggac ccccgtcgaa caccacctcg aacaggtgcg gctgcgccag cgccccgcgc
     1141 tggtaggcca gtccgagccg gaccaggtcg tcgaggctgt cgtcgcccgg cgtcacggcc
     1201 aggtgctctc ggaacagccg caacgcctcg tcgaacaggg ccgccagcag gcctgtcttg
     1261 ccgccgaaca gtgagtagac ggccgtggtg gacgtgccgc agtcggcggc gagggcgcgc
     1321 aggctcagcc ccgatacacc gtcggcggcg actcgctcca gcgctcgacc gaggaggcgg
     1381 ccacgcagcg cctcgtcgtg gaccttgggc cggaccatcg gcgaaggtta gtaccccggt
     1441 gatcgggccc ggataccagt gctccggctc gagggtgtca gctctccagc agatcgcagg
     1501 cctcgacgcg cagcgcgtcg aggcggccct gtgctgcggc cttcgcggcg gccagcgcag
     1561 ccgcgtccgg taccggctcg acgacctcca ggtacgcctt gagcttcggc tcggtgcccg
     1621 acgggcggat caccaggcgt tcgccgtcgc gccgcagcac gaggacgtcg ggcgcggggt
     1681 actcggccga ccagccggtg ggcgggccgg accggagccg ctcgaccacg gcgtcgcgct
     1741 ccgcgggatc cctccgcagc gacaggccct cggtgcggtg caccccatgc gcgacggcca
     1801 gctcgtcgag ccggtcggtc aggctccgtc cagccgcctt cagccctgcc gccagatcac
     1861 acgcgagcac ggcggccgcg atgccgtcct tgtcgcgcac cgcgtcgggg tcgacgcagt
     1921 agcccagcgc ctcctcgtag ccgtacacca gcccggcgcc gccccggacg atccacttga
     1981 accccgtgag ggtctcggcg taccgcgccc cgtacgcggt ggcgagcggg cggagcatcg
     2041 acgaggagac gacggtggtc gccaccagcg ggtcgcggta cggaacgccg tgagtgtccc
     2101 cggagcgcag cagatggtcg cccagcagca cgccgctctc gtcgccggtg agcaaccgcc
     2161 aggcgctccc gccctcgccc tcgccgaagc ccgcaaccgg cacggccagc gcgcaccggt
     2221 cggcgtcagg gtccagggcg atcgcgaggt cggcgcccac ctcggtggcc agcgcgagca
     2281 gggcgtcggt ggccccgggc tcctcagggt tggggaagga caccgtcggg aagtcgggat
     2341 cgggcattgc ctgcgctgcg acgacgtgga cgtcggtgaa cccggcccgg tgcagcgcgt
     2401 gcaccgcggt cgcgccgccg actccgtgca gcggggtgag cgccacccgc agctgcctgg
     2461 ccgggccacg cggcagctgc ccggctcggt ccaggtaggc ctccgcgacg tcgacggtcg
     2521 agaattcgcc gctggtcgga atcgagaccg cggccggggt ggcgttgatg gccgcctcga
     2581 tctcggcgtc cgacggcggc gccagctggg ctccgtcggc caggtagacc ttgtagccgt
     2641 tgtcggccgg cgggttgtgc gacgccgtga tctgcacgcc ggctacggcg ccgagctcgc
     2701 ggacggcgaa tgccagcagc ggggtgggca gcggcgcggg cagtgccagc acccggaagc
     2761 ccgcggcggc cagcacctcc gcggccgccg tggcgaacgc ctcggagccg tgccgtgcgt
     2821 cgcggccgat cacgaccgaa ccccgctcgc ccccgcgggc cagccacgcc gccagcccgg
     2881 ccgttgcgcg gcggacaacg gccacattca tccccgcagg gccggcccgc accggtccgc
     2941 gcagtccggc ggtaccgaag cgcagcatcc ccgccatccg gtcgtggagg tcgtcgaccg
     3001 cccccggcgc atcgaccatg gccgctgcca gcacccgctg cagctccatg cgcgtctcgg
     3061 ggtcggggtc gtcggcgatc caccgcatcg ccgactcccg taggtcgggc ggcagggtca
     3121 cgagcgggcg atcaggtcgc gcagcagctc acccatgcgt cctgccgccg ccgcacctgc
     3181 ggccagcacc tcgtggtggt cgagcggctc accggtcatc ccggcggcca ggttcgtcac
     3241 gagcgacagg ccgaacacct cgacgccctc ggcgcgggcc gcgatcgcct cgagcaccgt
     3301 ggacatcccg acgaggtcgg cgcccaacct gcgcagcatc cggatctcgg ccggcgtctc
     3361 gaagtgcggg ccgggcagac ccgcgtagac gccctcggac agtgtcgggt cgatctcccg
     3421 ggcgagcgcg cgtagccgcg ccgagtagag gtcggtcagg tccgtgaagc gcgggccgtg
     3481 cagcgacgag cgtcccgtca gattcaggtg gtcggagatc agcaccggct cgcccacccg
     3541 catgccctcc cggataccgc ccgccgcgtt ggtgagcacc accgtgcggc agcccgccgc
     3601 cgcggccgtg cgtacgccgt gtgccacggg gtcgacgccg tggccctcgt agaggtgcgt
     3661 gcggccgagc agcacgagcg cccggtgccc gccgacgggc accgaccgga tggtgccacc
     3721 gtgccccgcg acggcggggg ggacgaaacc gggcaggtcc gccatcacga tctcgtgcgc
     3781 cggctcgccg atcacgtcgg ccgcaggccg ccagccggac ccgagcacga ctgccacgtc
     3841 gtgggcgtcg acgccggtgg cggcggcgat ggcggcggcc gcggcgacgg ctggtggtgc
     3901 ggaggcgtcg acgctcatgc gcggagccta cgttcggccc ggacggccgc cggagtcagg
     3961 cgggcgtgga cgtgaagccg ccggcgacca gctcgaacag ggccgcggag gtggatccgg
     4021 cggcgacccg cgggacggtc agcgtgatcg tccacacgcc cttgtcggcg ggcaggatgc
     4081 gccgccagga cgtgtagtcg ccggactcgt accgcagctg tacggcatcg gccggggcag
     4141 gcgcaggtgc gggtgcgggt ggatcgagca acgttcccgc cacggcctgg gcggcctccg
     4201 tggagtcagc ccgctgcacg gcgagctcct cggtgcgggc cgagctgacg aagtcgacgc
     4261 tcggcagtcc accggccggc tcgttccgct gctccgtcca gtcctgggga accgggatgc
     4321 tgaagcccag cgcgtccgtg tgctcgagca tggccgtgct ggtcgaggtg acgttcaccg
     4381 tggtcagcgg cgactggccg cccaccagcc gggtgaccga ccacccgccc agtgcaccgg
     4441 ccagcacgac cgcggcaccc gcgacgacca gcccggctgc gctccatccc gacacggact
     4501 ccgaggagac cgagtgccgc gccggggccg ccgtgctcgc ggcccgcggc gccgctgccg
     4561 gtcggttgcg gaccggcggc aagggaccgg gcgacgtcgc caatggggcg gctgtctccg
     4621 ccggccctcg cgcgccggtc ggcggcggtg gcaggggagg cgggcgcccc ccggcgaact
     4681 cgaagcgcgc cgccgagctc ggcacggcga gcccggaccc gatcgtcggt gcatcgggcg
     4741 acccggggta gagcgggtcg tcggggtcgt cgatgagcgg ccgcagccgg tgccggacct
     4801 cctcgagcgg catccgggcg tccgggtcct tgaccatcag cgccatgatc acgtccgcca
     4861 ccgggctgga cgtccgcggt cgcggtacct cgccgtccac cacctcggtg atcgtcgaca
     4921 ccgggtcgcc gtgcacgtcg tacggcgggc gcccctcgag ggcggcgaag agcgtggcgc
     4981 cgaggcccca gaggtccgca gccggagtca ccggctggcc ggccgcgacc tccggtgcga
     5041 tgtacgcggg ggagccgagg acgaggccca cgctggtcat gggcgcgtcg gcgatgttgc
     5101 gggcgatgcc gaagtcggtg agcttgacgc ggccgtcgtc ggcgacgagg acgttgcccg
     5161 gcttgacgtc ccggtgcgtg atcccgctgc ggtgcgccgc ccggagcccg cctgcggtcg
     5221 cgaacccgac gaccgcggtc tgctgcagcg tcagcgtccc ctgctcaccg atcatcgtgg
     5281 ccaggttgcg cgacggcacg agctccagga cgaccatcgg tgcgccgtcg acgtcgacga
     5341 cgtcgaacac cgtgatgatg ttcgggtgcg acaggccgcc gagtgcccgc gcctcgcgca
     5401 tgatgcgttc gcgcgcctcc gccgcctcct gctcgggtac gccgggcggg accttcagct
     5461 ccttgaccgc cacccggcgc tgcagcacct cgtcgtagcc cgcccacacc gtgcccatgg
     5521 caccgctgcc gagcagatcg gacaaccggt accgcccacc gattcggcgc acgtgctggt
     5581 cttccgggct gtcctgcggc acccgcccat tctgacgagc ccgcacccgg gtggcgctgc
     5641 cgccccccac gtcctggccc aatgcctcac tccacgtggt cgcgcgggcc gtcggacagt
     5701 gcgtgggaga ggtccggacg gggtaggacg acgactgtgc gctacgcaga agattgccgg
     5761 gacggacgac gagcggtgcc ggcccccgtt cgtctcggca tccggcgacc gttcgtcact
     5821 gcatacgggc ccgcccgcag cagatccgca acccctcggc gaggatttcc acccgaggcg
     5881 cgattccggt tggtacaccg ccgtccgggt gagcaccatg gtcagatgcc cgacagatca
     5941 gcctcgaggc gcgcaccgct gacgctgcgc gctgcgaccg gtctctcgac gaccaccgca
     6001 cagggtggtg tgaggtgact ttgctggagg cccataccga cctcgtgcca cccgcggagg
     6061 ctgccgtcac cgacttcgga gcgggcagcg gaccggcatg gctcgactcc tggctactgg
     6121 cccaccgcag cgacgtggtg gcctggcggc gcgccctgca cgccttaccg gagctcggac
     6181 gcgtcgagca ccgcaccacc gagctgcttg cgcggcgact gatggcggcg ggcctcgagc
     6241 cgcgtaccct ccccggcggc accggcctcg tctgcgacat cggctacggc gaccgctgcg
     6301 tcgcgttgcg tgccgacctc gacgcccttc ccctgcagga ggacacgggc ctgccctacg
     6361 cgtccacggt cgacggtgtc atgcacgcct gcgggcacga cgcgcacgcc gcaatgctgc
     6421 tcggggccgg cctcgcactc gcttccgcgc cggcgttgcc cggccgcgtg cggctcatct
     6481 tccagcccgc cgaggaggtc cagccgggcg gggcgatcga catggtcgcc gacggcgtca
     6541 tggagggcgt ccagcgcgtc ttcgcgctgc actgcgaccc gcggctggag gtcggcaagg
     6601 tcggcacgag ggtcgggccg atcacgtcgg cctgcgacgt cctggaggtc cggctcacct
     6661 cgccgggtgg ccacacctca cggccacacc tcacggccga cctcgtcgag gccctcggga
     6721 tcctcatcac gcaactgccg ctgctcctca cccggcaggt cgacccgcgc tcgggcaccg
     6781 tgctcgtctg gggcgcggtg caggcgggcg aggcggccaa cgccatcccg cagcatggtg
     6841 ttctgcgcgg cacgctgcgc acggccgacc accacacctg gggggagctg gagggcaagg
     6901 tccgtgcact ggtcgcggcg ttgctcgcgc ccaccggaac cgggtacgtg ctcgagcaca
     6961 tccggggcgt gccacccgtg gtcaacgaag ccgtcagcgc cggcatgctg gccgacgccg
     7021 ccaccgccgt cctgggcccg gacgccgcca ccagcaccga gcagtccagc gggggcgagg
     7081 acttcgcctg gtacctggag cacgcgcccg gcgccatggc gcggctcggg gtgtggcccg
     7141 gccacggccc gatgcgtgac atccaccagc ccacgttcga cctcgacgag cgcgccctcc
     7201 ccttcggcgt ccggatgcac gtccaggtag ctctggccgc cctgaccacc tagccaaccc
     7261 acccgcgagt cgcccgaatt cggcccgcga gccgcccggt ctggccccgc gagtcgcccg
     7321 gattcggccc gcgtgtcgcc ccgatcaggc cgccagtcgt ccagacgctg gtgactcccc
     7381 ggtgtggccg gggatcccgg cgacgcaggt gtcggcgtgc gtggcccccg agccgcccgc
     7441 gtcaggcagc ttgtgtccgg agtggatacc cggaggtcga cctgggccgg tccagctggg
     7501 cgaccgtgct gccgcgggtg cggcggcccg gcggacgggg tcggtcggct gctgctcaga
     7561 cggacttgcg gctggggcgg ctgcgcagcg ccgagacgta gtcgtcaggt gcgccggcct
     7621 gttcggcggc gtcggcgatc accccgaggt agcgagcgct cggttccccg ccctcgtagt
     7681 cgttgagcac gtagatccac gccagcacgt cgccctcgag ggtgtggacc cgcaggcgta
     7741 gcttcttgtg caacccgagc tcaccgccct cccagcggtc cagctgggcg gagtcgtggt
     7801 cgggcacgtc gtagaggacg acgaacacct gcgaaccggg ctcctcgacg acggtggaga
     7861 gcgcgccctc ccagccgtag tcctcgccac cgaaggtgag ccgccagtcg acgagccacc
     7921 cggtacccgc catcggcgag tgcggggcac gctccttcat ctgcgcggga tccatgttcg
     7981 acccgtaggc ggcgtacaac ggcaccgcca cagagtagtg cgccgccgcc cgcgtaccgt
     8041 tccgggggaa ccggacatct ggaggaggca tgcctcggtg actcgcatcg tgatcatggg
     8101 cggggggccc gcggggtacg aggcagcgct ggtggcagca caacacggca gcgatgtcac
     8161 cgtcgtcgag cgggaaggca tgggcggcgc gtgcgtgctc gacgactgtg tgccgtcgaa
     8221 gaccctcatc tcgtcggccg gtatccgggt cgacctgcac cgcgcccctg acctgggcct
     8281 cattgccgac cggcaggcga tcggggtcga cctgcccacc gtgcacgccc ggatcaagcg
     8341 cctcgcgctg gcacagtcgg ccgacgtgcg agcccgtgtc gagcgcgagg gcgtccgcat
     8401 cgtccgcggc acgggcgtct tcaccgacgc gccgtccgta cgggcgcccc acgtcgtcga
     8461 gacccggacg ctcgacggcg gccgtgaaga gctcccggcc gatgtggtgt tgatcgccac
     8521 gggtgccacc ccgcgcgtgc tcgacgacgc gaagcccgac ggtgagcgca tcctcacctg
     8581 gcgccagctc tacgacctcg aggcgttgcc cgagcacctg atcgtggtgg gctccggcgt
     8641 caccggagcg gagttcgtgt cggcgtacgt cgagctcggg gcgcgtgtca cgctggtgtc
     8701 cagccgcgac cgcgtgctgc ccggggagga cgccgacgcc gcagcggtgc tgcaggacgt
     8761 gttcgccgag cggggcgtgg agatcctctc ccaagccagg gccgactcgg tgcgccgcga
     8821 cggggacggg gtcgtcgtca ccctcgccga tgggcgcacg gtcaccggct cccacgcgct
     8881 catgaccgtg ggctcggtgc ccaacaccgc cgacctcgga ctggagaaga tcggcgtcga
     8941 gaccgggcgg ggcggtttcg tcccggtcga ccgggtctcg cgcacgtcgg tacccggcgt
     9001 ctatgcagcg ggcgactgca cgggcgtgct gatgctcgcc tctgtcgccg cgatgcaggg
     9061 ccggatcgcg atgtggcacg ccctaggtga gggcgtcgca ccgatcaagc tcaagacggt
     9121 ggccgccgca gtgttcaccc gcccggagat cgcgaccgtc gggatcagcc agaatgcgat
     9181 cgacgcaggc gaggttccgg ctcgcacgat catgatgccg ctgtcgacga acccgcgggc
     9241 gaagatgcag gggttgcgcc gcggtttcgt caagctgttc tgccgccccg ccacgggcgt
     9301 ggtcgtgggc ggtgtcgtcg tcgcgccggt cgcgagcgag ctgatcctgc cgatcgcgct
     9361 ggccgtgcag aacgggctca ccgtcgccga cctggcccac accttctcgg tttatccgtc
     9421 cctgtccggt tccgtcaccg aggcaggccg ccagctgatg cgccacggcg acctggactg
     9481 aggacgcacc tcacgcgcgg ccgctcgcca gctcgcgcag cgccgcgaag ttcaccgtcc
     9541 atccgacgcc gaacgggtgc ggcaccagca cggccggatc gtcggggttc cacagcctgc
     9601 tccgtaaccg gtcgaacgtg ggcggccgca ggtcgtaggg aaccaggccg gcgacgctcc
     9661 cgtgccacgt ccgttcgacc gggggcttgc gcagctcggt cgcgacagcc gcgcccacca
     9721 gcacggccgc tgcggctgcg gcggtgcgat ccacgcctct tgccatgctg ggcgacgata
     9781 cgccgaccgc cgccggtgac cggtgccccc gccgagcgac cctgggttcg gctccggggg
     9841 atccccgatg ccggtcgcag cggcggcgcg gacgatcggt gccatgacca ctgtgcctgc
     9901 cacgtcccgg gtgcccgccg gcgccgtcct gctcgccggg ctcggcgcgc agctcgtgga
     9961 cgacgccgtc ttcctcgtcc tcccgccgac cgcgctgctc gccccgctga gcgggctcct
    10021 cgcgatactc ctcaccgccg gtgccgcgcg gatcgtcacg cgggaccgca ccggcgcggt
    10081 ggtggcccgc acgggcctcg ccgtcggcct gacgtcggcg gtgatcgggc tcctcgtcgg
    10141 cggcctaggc atggtggcga tcctgctcgc cggcgtcacc gtcgtggccg gggtggccgg
    10201 tgcggtggcg gggcgcggcc tgaccaagaa cgacatctcc tgacgcgccg cggcgcccgg
    10261 gcggcggagg ccatccacgg tcagggggct cgcaggccag atacgggcga ctcgcgcggg
    10321 ggcctcgcgg gcggcgactc gcggggcggg gacgggggtc gactcgcggg cgggaagggg
    10381 cgactcgcgg gcggaaagag ggcgactcgc gggcgggaag ggggcgactc gcgggtcagg
    10441 tttggctggt gggtgcgagt agttcggcga ccaggtgccg taaccggcgg gcgtcggtgg
    10501 gggcgacggt ggcccactcg acgccgtcct cgccgaccgt gcgggtcatg aggtagcggc
    10561 cgcggggtcc gtcgagtacc gcgagaacac cgcccgagcg acggagcacg ccccagcggt
    10621 cgctgaccag ggcgacgatc tgcgcgcgcc cctcgatacc gatgagcatg cgggcgagga
    10681 gacccgcgtc gacggacgga acgtgcttgg cgaggagtgc tgaccgcagc ccggcgccct
    10741 gagctgcggt ggccgctgcg agcacagcgg ccggcacggt gctcgcccga ccggggcccg
    10801 gcccggcctg cgggagcgcg cggagcagcg cggacgggag tgagcggtgc gatccgagcg
    10861 tgacggtgtc gtcctggcgg acggccagca cacatgcccc gcgcgcgccg gcggccacgg
    10921 cccggacgct gcggtcccac caccctcgca gctcgagttg ctctgcaggc cgggccagca
    10981 ggtgcagcag gcggacgagt tcggggtcgg cgccggcggg gccggccagc ccgcgctcac
    11041 gcagcgcctg ccagcctgcc gccaggacgt cccgccgctc ggcatgggtg cggccgggcg
    11101 acgcgagccg caggacgacg ggggtggcgc ccagcccgag gcgttcccac aacacgtcga
    11161 actcgacggc ggtgagcacg tgccggtgca tgtgcggcgg gtcaaggacg agaggcggct
    11221 cggtcatggc ggtcctccca cggggcggtc tccgaccgcg acggtaggcg gggaggtgcg
    11281 gtggcgtggg cgttcgacga actcaattgc gatcgcgaag atcgctgcgg gccggccgcg
    11341 acggccgatg cggttgcagt ccgcaccggc cgtcgcgggt gtccacctct cgtcagctcg
    11401 tccgggccag tgacggctcc ggcatccgac atccggcgag cgggcagagg gtcgagcagc
    11461 tcatgcggcc gtggaggctc gcatcggtga gcgtgctggc gaagggccag cgcccggtga
    11521 agcgttggag ataccagtac aggaacagat agtcgggttc tgcggtgtcg gcgacgaaca
    11581 ccgggcgccc tggcagctga tcgatccatt gggcgaaggc ggtcatcgcg agggcgggtg
    11641 gacggctcgc ccggcgggtg gagagccagt cctccgcccg tcgcctccag ctctggagag
    11701 cgaccgggtg cagggtggca ccgggc
//
LOCUS       DASRSC010000242         8589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5167737,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000242 DASRSC010000000
VERSION     DASRSC010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5167737"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..691)
                     /locus_tag="VFE39_11770"
     CDS             complement(<1..691)
                     /locus_tag="VFE39_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6259319.1"
                     /translation="MRIAFVGKGGSGKTTSAAMFSRYLAELGHPVLAIDADINQHLGS
                     ALGHDGPPPRALGTDLTWLKDHLRGTNPRITSADVMIKTTPPGSGSRLLDLAPDGELL
                     RRYSAVAGGVRHLATGEFDEADVGVSCYHSKTGAVELYLNHLVDGPGEYVVVDMTAGA
                     DAFASGLFTRFDLTVLVSEPTRRGVGVYRQYAEHAAGHGVALRVLGNKVSDPDDVAYL
                     REQVGDALLGWF"
     gene            complement(788..2086)
                     /locus_tag="VFE39_11775"
     CDS             complement(788..2086)
                     /locus_tag="VFE39_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259320.1"
                     /translation="MNVHHRAWGVWAVGLAAYLVGVMHRTSFGVAGLDAATRFHAGPA
                     VLSGFVVLQLLVYASLQVPVGVLLDRFGARLLVAVGAITMAAGQLMLALATALPLAIL
                     ARVLVGAGDALSFISVLSVVTAWFPARRVPLMTQLTGLIGQLGQVLSAVPLAALLHGP
                     GWTTAFVSAAALGVAVAIAVLGVVRDRPPGAPAPPQAPSPREVLVGLASSWREPGTRL
                     GLWTHMGTQFSGTVFSLLWGVPYLVAGQGFSPGEASVMLTVLVAVGIVAGPLFGEFTA
                     RHPLRRSWLVLAVIGLTSIMWTLVLLVPPPAPRWMLVLLVIVLALGGPASMVGFDYAR
                     TFNPGHRQGTAVGIVNVGGFVASLLVALAVGVVLGLIGPADGYTPEAFRGAWTVQYVI
                     WGLALVGVLVARSRARRKMAFEGVLVPPLREVLADRRRAR"
     gene            2243..3220
                     /locus_tag="VFE39_11780"
     CDS             2243..3220
                     /locus_tag="VFE39_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HET6259321.1"
                     /translation="MLEPAELYEVAADAPQLDEPVLLVALEGFVDAGNAARLAVEALL
                     ADREARNVVRFDADQLVDYRSRRPPLRFETDHWAAYDPPELDVVALSDTADTGYLLLS
                     GPEPDTQWERFSAAVEQIVRRLGIRLVINLTAIPMAVPHTRPIGVTAHGTRPELTEGH
                     EAWFATAQVPGSAVGLMEFRLGEAGLDAMGFAVHVPHYLARAEYPQAARVLLDHVGLA
                     AGLYLPTEVLLKAAERAEAEIAEQVEGSDEVRQVVQALERQYDTVSTGRGERSSLGLS
                     GTLPSADELAAEVERFLAGHGDADNSDDNNSDDNSDDNGDEGRGGAQPS"
     gene            3327..3785
                     /locus_tag="VFE39_11785"
     CDS             3327..3785
                     /locus_tag="VFE39_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4262 domain-containing protein"
                     /protein_id="HET6259322.1"
                     /translation="MCWMCDDPTLQPEQYLEHTARLIEEHGWVVQGVSGRRDRPPWSY
                     TVGLTSHHRPELVITGLPAPRAATLLDGVASHLLHATEPPPGEQTRLVGGPLVEFVAV
                     EHPDVHLVVAQVLYGPAVRALQVVWADDRDHWPWHPGFRGRQPVLGPRAS"
     gene            complement(3836..6211)
                     /locus_tag="VFE39_11790"
     CDS             complement(3836..6211)
                     /locus_tag="VFE39_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase"
                     /protein_id="HET6259323.1"
                     /translation="MTSIEARRDTASDASRTDPAAVLREIERRVLWLATAIVHHANRV
                     RPNPSGLKVGGHQASSASIATIMTALWFEHLRSGDRVSVKPHASPVLHAINYLLGELD
                     ERYLTTLRAYAGLQSYPSRAKDPDPVDYSTGSVGIGATAPIWGALARRYVTSLGADPG
                     MGRQYSLVGDAELDEGACWEAILDPMVGELGEIVWIVDLNRQSLDRVVPNIGASRLQG
                     MFDAAGWQVLTVKYGRLLEELFTRPGGAALRRRIDEMPNPEYQRLLRCTADQLRERLP
                     GDDPALRGLIGELDDATLHAAVRNLGGHDLAALRDAFTAIDDARPTVVFAYTVKGYGL
                     ASEGHPQNHSSLLTPEQMTELAGAVGASLEDPWARFPHGSAAAEVCESVATRLRREPV
                     ASAAVAPIPADIGRTPAGTATTQAALGRALLDLTRSAPEAARRVVTLCPDVSSSTNLG
                     GWVNKVGVWSARERVDWFADDPETILHWRERPTGQHLELGIAETNLVGLLGELGATWS
                     RWGEPLLPIGVLYDPFVERALEPWSFGIYAGGQSILVGTPSGVSLAPEGGAHQSISTP
                     SVGLEQPGCISYDPAFAIDVEWTLLASLARLGRPDGTSAYLRLSTRPVDQTLADVPTD
                     PAARDRRRRQVVAGAYPLYRAGTGCAGTGRAGTGTATEPALTVAAMGAVVPEALAAAE
                     RLDALGYPTDVVCVTSAGLLFDALQARSGHGDGETWILDAAFPARRATPMVTVLDGHP
                     HTLAFLAGINQVRAAHLGVSRFGQSGDLDDVYRHHGLDADSIVRAALDLAP"
     gene            6338..6820
                     /locus_tag="VFE39_11795"
     CDS             6338..6820
                     /locus_tag="VFE39_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET6259324.1"
                     /translation="MRTTGGVDATDARLLLALTENPRASVLALAERLGLSRNTVQARL
                     AGLEARGAVTSFERRIDPAALGYPLTAFVTVQVVQRRLAEVAAALDGVPEVIEVLGLS
                     GQADLLVQVVARDADDLYRIAGQLLATEGVERTTTSLVMRTLVEHRLAPLLKRLAGQA
                     "
     gene            6869..7276
                     /locus_tag="VFE39_11800"
     CDS             6869..7276
                     /locus_tag="VFE39_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HET6259325.1"
                     /translation="MTDNTTPDLPSEIVEMLGHPNPAVIGTVRRDGAPVTVATWYLYR
                     DGRILVNMDAERRRLDHIRHDPRVSITVLDGEDWGTHVSMQGRLTLQDDPDLQVLDMI
                     ARHYTGKPYPQRDRPRVTGVLEIEAWHGWGRFA"
     gene            7481..>8589
                     /locus_tag="VFE39_11805"
     CDS             7481..>8589
                     /locus_tag="VFE39_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HET6259326.1"
                     /translation="MTVSETPRSSETPRSSETPGSSDPTAADATSGRGSAEQRYPVDR
                     WVFGVAAALVVAFIVWGTLSTDTLSAVATAVLGGVMNAGGWAFVLAASGFVVFALWLA
                     MSRYGKIPLGRDGEKPEFRTVSWVAMMFSAGMGIGLMFYGVSEPLAHFTNPPPGTVPA
                     NSAEALDVAMATTLFHWTLHPWAIYAVVGLAIAYSTFRRGRRQLISSAFAPLLGERRS
                     EGAFGKAIDILAIFATLFGSAASLGLGTLQIGGGLQANGLPPLGDLLLVSIIALLTIA
                     FVCSAVTGVAKGIQWLSNINMVLAAVLAVVVFVAGPTVLILNLIPTAIGDYLGNMAQM
                     AARTAATGGDATAAWLASWTVFYWAWWISWTPFVG"
BASE COUNT         1302 a         3065 c         3067 g         1155 t
ORIGIN      
        1 cgaaccatcc gagcagggcg tcgcccacct gctcgcgcag ataggcgacg tcgtccgggt
       61 cgctcacctt gttgccgagc acacgcagtg ccacgccgtg tccggcggcg tgctcggcgt
      121 actgccggta gacgccgaca ccgcgccggg tcggctcgct gaccagcaca gtcaggtcga
      181 agcgggtgaa caggccggag gcgaaggcgt cggcccccgc cgtcatgtcc accaccacgt
      241 actcgccagg gccgtcgacg aggtggttga ggtagagctc caccgccccg gtcttcgagt
      301 ggtagcagga gacgccgacg tcggcctcgt cgaactcgcc ggtcgccagg tggcggacgc
      361 cgcccgccac cgccgagtac cgccgcagca gctcgccgtc cggggcgagg tcgagcagcc
      421 gcgacccgga gccgggcggg gtcgtcttga tcatcacatc ggcggaggtg atgcgcgggt
      481 tcgtaccgcg cagatgatcc ttcagccagg tgaggtccgt cccgagcgcg cgcggtggcg
      541 ggccgtcgtg gccgagagcc gaaccgaggt gctggttgat gtccgcgtcg atcgcgagta
      601 ccggatgacc gagctcggca aggtaccggc tgaacattgc cgccgacgtc gtcttcccac
      661 tgccgccctt gccgacgaac gcgatccgca cggtccctcc gatgatcgtc ttccgaagcc
      721 ggcgctaatg ataagcgtta tcactatcgg gtgacggcat cgcctcggtg cgggacggga
      781 tcatcggtca gcgcgcgcgg cgccggtcgg cgagcacctc gcgcaacggg ggcaccagca
      841 cgccctcgaa cgccatcttt cggcgggccc tggacctggc gaccagcacg ccgacgagcg
      901 cgagccccca gatcacgtac tggaccgtcc acgccccccg gaacgcctcc ggggtgtagc
      961 cgtccgccgg cccgatgagc ccgagtacga ctcccaccgc gagcgccacg agcagcgacg
     1021 cgacgaaccc gccgacgttg acgatcccga cagcggtgcc ctgccggtgg ccggggttga
     1081 aggtgcgggc gtagtcgaaa cccaccatgg acgccggtcc gccgagggcc agcacgatca
     1141 cgagcagcac gagcatccaa cggggggccg gcggcgggac gagcagcacg agcgtccaca
     1201 tgattgacgt caagccgatc accgccagca ccagccacga gcgccgcagc gggtgccggg
     1261 cggtgaactc gccgaagagc ggccccgcga cgatccccac cgcgacaagc accgtgagca
     1321 tcacgctcgc ctcgccgggc gagaagccct ggcccgccac caggtacggc acgccccaga
     1381 gcagcgagaa cacggtgccg gagaactggg tgcccatgtg cgtccacaac ccgaggcggg
     1441 tgccgggctc gcgccatgac gaggcgagcc caacgagcac ctcgcgtggt gacggcgcct
     1501 gcggtggtgc gggtgcaccc ggcggacggt cgcgcaccac cccgagcacc gcgatcgcga
     1561 cggccacccc gagcgccgcc gcggacacga acgcggtggt ccacccagga ccgtgcagca
     1621 gcgcggccag cgggacggca ctgagcacct gcccgagctg cccgatcagg ccggtgagct
     1681 gtgtcatgag cggcacccgg cgcgctggga accacgccgt gacgaccgag agcacgctga
     1741 tgaagctcag cgcgtcccct gcgccgacca gcacccgcgc gaggatcgcc agcggcagcg
     1801 cggtggccag cgcgagcatc agctgtcccg cggccatcgt gatcgccccg accgcgacga
     1861 gcaggcgcgc cccgaaccgg tcgagcagca cgccgacggg tacctgcaga ctcgcgtaga
     1921 cgagcagctg cagcaccacg aaacccgaca gcacggccgg accagcgtgg aacctggtcg
     1981 cggcgtcgag gccggctact ccgaacgacg tgcggtgcat gacgccgacg agataggcag
     2041 ccagccccac cgcccagacg ccccaggcgc gatgatgcac gttcatgtca gttccatcgt
     2101 gcgtgcggaa acgcccgaac ggggatgctg tgcggggaaa ttcacgttgc accggtgaga
     2161 cctgatcgca agggcgaacc atcggccgtg cgacgtgcga tgggcggggc ccggtgcccc
     2221 gtcgagaaac cggaggtgtg cggtgctgga gccggccgag ctgtacgagg tggcggcgga
     2281 tgcgccgcag ctggacgagc cggtgctgct ggtggcgctg gagggcttcg tcgacgcggg
     2341 gaacgcggcg agactggccg tggaagcgtt gctggcggac cgcgaggcgc ggaacgtggt
     2401 gcgcttcgac gccgaccagc tcgtggacta ccggtcgcgg cgcccaccgc tgcgcttcga
     2461 gaccgaccac tgggctgcct acgacccgcc cgaactcgac gtcgtggcac tgtcggacac
     2521 cgccgacacc ggatacctgc tgctctccgg tcccgaaccg gacacgcagt gggagcgctt
     2581 ctctgctgcc gtcgagcaga tcgtgcggcg actcggcatc cgtctggtga tcaacctcac
     2641 ggccattccg atggccgtgc cgcacacgcg gccgatcggc gtgaccgccc acggcacccg
     2701 cccggagctc accgagggcc acgaggcgtg gttcgccaca gcgcaggtgc cgggcagcgc
     2761 ggtggggctg atggagttcc gcctcgggga ggccgggctc gacgcgatgg gcttcgcggt
     2821 acacgttccg cactacctgg cccgagcgga gtacccgcag gccgcgcgcg tcctgctgga
     2881 ccacgtcggg ctggccgccg ggctctacct gcccaccgaa gtgctgttga aggccgccga
     2941 acgcgccgag gcggagatcg ccgagcaggt ggagggctcc gacgaggtgc gccaggtcgt
     3001 ccaggcgctg gagcggcagt acgacacggt gtccaccggt cgcggcgagc ggtcgagcct
     3061 cgggctctcc gggacgctgc ccagtgccga cgagctcgcc gccgaggtcg agcggttcct
     3121 cgccggccat ggcgacgccg acaacagtga tgacaacaac agtgacgaca acagtgacga
     3181 caacggcgac gaggggcgcg gaggggcgca gcccagctga accggccctg tggacaactt
     3241 ctcgccggcg cgcggcgtac gcccgtcgcc gtcggtggtc acggtcatcg tggcccggtc
     3301 ggagtcgacc gaccgaggag gaaccgatgt gctggatgtg cgacgacccc accctccaac
     3361 ccgagcagta cctggagcac acggctcggc tgatcgagga gcacggatgg gtggtgcagg
     3421 gtgtgtcggg ccggcgcgac cgcccaccgt ggtcgtacac agtgggcctc acgtcccacc
     3481 accggcccga gctcgtgatc accgggctgc cggcgccccg ggccgcgacg ctgctggacg
     3541 gggtcgcgtc gcacctgctg cacgccaccg agccaccgcc cggtgagcag acgcggctcg
     3601 tcggtgggcc attggtcgag ttcgtcgcgg tcgagcatcc cgacgtccac ctcgtcgtgg
     3661 cccaggtcct gtacgggccg gccgtgcgcg cgctgcaggt ggtgtgggcc gacgatcggg
     3721 atcactggcc atggcatccc ggcttccgcg gccgccagcc ggtgctgggc ccccgggcga
     3781 gctgaccgtg ccgggtccgg agcgacacga acgcggacga tcgggatggg ccgggtcagg
     3841 gagccaggtc cagggccgcg cggacgatgc tgtcggcgtc gaggccgtgg tgacggtaga
     3901 cgtcgtccag gtcgcccgac tgcccgaacc gggagacgcc gaggtgcgcg gctcgcacct
     3961 ggttgatgcc ggccaggaag gccagcgtgt gcgggtgccc gtccagcacc gtgaccatcg
     4021 gcgtcgcgcg gcgggccggg aacgcggcgt cgaggatcca ggtctccccg tctccgtgcc
     4081 cgctgcgggc ctgcagcgcg tcgaacagca acccggcgct cgtcacgcac acgacgtcgg
     4141 tggggtagcc gagcgcgtcg agccgctcgg cggccgcgag agcctcaggc acgaccgcac
     4201 ccatcgcggc gacggtcagc gcaggctccg tggcggtgcc ggttcccgcc cgacccgttc
     4261 ctgcacaacc cgttcctgcc cggtacagcg ggtaggcccc ggccaccacc tgtcgccgac
     4321 ggcgatcccg cgcggcaggg tcggtcggca cgtcggcaag tgtctggtcc accggccgtg
     4381 tcgagagccg caggtaggcc gaggtgccgt ccggccgccc gagccgggcc agcgacgcca
     4441 gcagtgtcca ctccacgtcg atggcgaaag cggggtcgta actgatgcaa ccgggctgct
     4501 ccagccccac cgacggcgtg ctgatcgact ggtgcgcccc gccctccggc gccagcgaca
     4561 ctccggaggg cgtgccgacg aggatcgact ggccgccggc gtagatcccg aacgaccagg
     4621 gctcgagcgc acgctcgacg aacgggtcgt agagcacccc gatgggcagg agcggttcac
     4681 cccagcggct ccaggtggcg cccagctccc ccagcagtcc cacgaggttc gtctcggcga
     4741 tgcccagctc caggtgctgg ccggtgggcc gctcgcgcca gtgcaggatc gtctcgggat
     4801 cgtcggcgaa ccagtccacc cgctcgcggg ccgaccacac gcccaccttg ttgacccagc
     4861 cgcccaggtt cgtcgacgag ctgacgtccg ggcacagggt caccacccgc cgggccgcct
     4921 cgggcgcgga gcgggtgagg tcgagcagcg cgcggcccag cgccgcctgg gtggtggcgg
     4981 tgccggccgg ggtgcggccg atatcggccg ggatcggcgc cacggcggcc gacgccaccg
     5041 gttcccggcg cagccgcgtg gccaccgact cgcacacctc ggcagcggcg gacccgtggg
     5101 ggaagcgcgc ccagggatcc tcgagggacg cgccgacggc cccggcgagc tcggtcatct
     5161 gctcgggcgt gagcagcgac gagtggttct gcgggtgccc ctcgctggcc aggccgtacc
     5221 ccttcacggt gtaggcgaag accaccgtgg ggcgggcgtc gtcgatcgcc gtgaaggcgt
     5281 cgcggagagc cgcgagatca tgacccccga ggttgcggac ggcagcgtgc agggtggcgt
     5341 catcgagctc accgatcagc cccctcagcg ccgggtcgtc gccggggagc cgctcgcgca
     5401 gctggtccgc cgtgcaccgc aggagccgct ggtactccgg gttgggcatc tcgtcgatgc
     5461 gtcgacgcag ggcagcaccg ccggggcggg tgaacagctc ctccagcagc cgcccgtatt
     5521 tgacggtgag cacctgccag cccgcggcgt cgaacatgcc ctgcagtcgc gatgccccga
     5581 tgttcgggac cacgcggtcg agcgactgcc ggttgaggtc gacgatccac acgatctcgc
     5641 cgagctcgcc gaccatcggg tcgaggatcg cctcccagca ggcgccctcg tcgagctcgg
     5701 catcgccgac cagggagtac tggcggccca tgcccgggtc ggcgccgagg gaggtgacgt
     5761 aacggcgggc gagcgcaccc cagatcggcg cggtcgcgcc gatcccgacg gagccggtgg
     5821 agtagtcgac cgggtcgggg tccttggccc ggctcgggta gctctgcagc ccggcgtacg
     5881 cgcgcagggt ggtcagatag cgctcgtcga gctcgccgag caggtagttg atcgcatgca
     5941 gcaccggcga ggcgtgcggc ttgaccgaga cccggtcacc gctgcgcagg tgctcgaacc
     6001 agagcgcggt catgatcgtc gcgatcgacg cgctcgacgc ctggtggcct cccaccttca
     6061 gcccgctggg gttcgggcgc acccggttgg cgtggtggac gatcgcggtg gcgagccaga
     6121 gcacccgtcg ctcgatctcc cgcagcaccg cggccgggtc ggtcctgctc gcgtccgaag
     6181 cggtgtcccg ccgcgcctcg atgctcgtca tcatgccctc ctgacaccat gttgcccgtc
     6241 aactggacca ccagcacgat gactgcacaa ccgaaggcgc tgtgattaga cataatgctg
     6301 agtccgaggt gatcgccacg ggaagggagc gagcagaatg cgcaccacag gtggcgtcga
     6361 cgcgaccgac gcgcggttgc tgctcgcgct caccgagaac ccacgcgcat cggtgctcgc
     6421 gctggcggag cgactcgggc tctcccgcaa cacggtgcag gcccggctgg ccggcctcga
     6481 ggctcgcggc gcggtcacct ccttcgagcg ccggatcgac ccggcagcgc tcggctaccc
     6541 gctcaccgcc ttcgtgacgg tgcaggtcgt gcagcgccgc ctcgccgagg tcgccgccgc
     6601 gctcgacggg gtgccggagg tgatcgaggt gctggggctg tccggccagg ccgatctgct
     6661 ggtacaggtg gtcgcccgcg acgcggacga cctgtaccgg atcgccgggc aactgctcgc
     6721 caccgaaggg gtcgagcgca ccaccacatc cctggtgatg cgcacgctcg tggagcaccg
     6781 cctcgccccg ctgctgaagc ggctcgccgg gcaggcctga gcaccgctcg gcgctgcgcg
     6841 ggcagccgtc ctcagtgacg atccggccat gacagataac acaacgcctg acctaccatc
     6901 cgagatcgtc gagatgctgg gtcaccccaa cccggcggtg atcggcaccg tccgccgcga
     6961 cggcgcaccg gtcaccgtcg ccacctggta cctctaccgc gacggacgga tcctcgtgaa
     7021 catggacgcc gaacggcggc gcctcgacca catccggcac gatccccggg tgtcgatcac
     7081 cgtcctggac ggggaggact ggggcacgca cgtcagcatg cagggcagac tgaccctgca
     7141 ggatgatccc gacctccagg tcctcgacat gatcgcccgg cactacaccg gcaagccgta
     7201 cccgcagcgc gaccggccac gggtcacggg tgtgctcgag attgaggcat ggcacggctg
     7261 gggccggttc gcctgagcgc aggcggccgg tcggcgcctg ccggccgcac caccgggccg
     7321 atcatgcgca atgccgcccc tgcttccaca acgagaaggt tgccgtcccg gcgctccggt
     7381 tgacgccgcc gccccgggag acgcagggtg cacccatccg cgaacttcgc ccgtttcatc
     7441 cgcacgaaag gggaggctcc gcccatcgga gccgcacaga atgaccgttt ccgagacacc
     7501 caggagcagc gagacaccca ggagcagcga gacacctggg agcagcgatc ccaccgcggc
     7561 ggatgcgacg tccggccgag gctcggccga gcagcgctac ccagtggaca ggtgggtctt
     7621 cggtgtggcc gcggcgctcg tcgtcgcgtt catcgtctgg ggcacgctgt ccaccgacac
     7681 gttgagcgcg gtcgccaccg cggtgctcgg cggagtcatg aacgcgggcg gttgggcgtt
     7741 cgtgctggcg gccagtggct tcgtggtgtt cgcgctgtgg ctggcgatga gccggtacgg
     7801 gaagatcccg ctgggccgcg acggcgagaa gcccgagttc cgcaccgtct cgtgggtcgc
     7861 gatgatgttc agcgccggaa tgggcatcgg gctcatgttc tacggcgtca gcgagccact
     7921 cgcccatttc acgaacccgc cgccgggcac cgtgcccgcg aactcggccg aggcgctgga
     7981 cgtggcgatg gccaccacgc tgttccactg gacgctgcac ccgtgggcga tctacgcggt
     8041 ggtcgggctg gcgatcgcct acagcacgtt ccgccggggc cgccgccagc tgatcagctc
     8101 cgcgttcgcg cccctgctgg gcgagcggcg cagcgaaggc gcgttcggca aggcgatcga
     8161 catcctggcg atcttcgcga cgctcttcgg ctccgcggcg tccctcgggc tcgggacgct
     8221 gcagatcggc ggtggcctgc aggccaacgg gttgccgccg ctcggcgatt tgctactggt
     8281 gtcgatcatc gctctgctga cgatcgcgtt cgtctgctca gccgtcaccg gtgtcgccaa
     8341 gggcatccag tggctctcca acatcaacat ggtgctcgcc gccgtgctcg ccgtcgtggt
     8401 gttcgtggcc ggtccgacgg tgctgatcct caacctgatc ccgacggcca tcggggacta
     8461 cctcggcaac atggcgcaga tggctgcccg caccgcggcc accggcggcg acgccaccgc
     8521 cgcgtggctg gcgagctgga cggtcttcta ctgggcctgg tggatctcct ggaccccgtt
     8581 cgtcggcat
//
LOCUS       DASRSC010000243        10696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1730033,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000243 DASRSC010000000
VERSION     DASRSC010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10696
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1730033"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VFE39_11810"
     CDS             <1..610
                     /locus_tag="VFE39_11810"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6259327.1"
                     /translation="DLAGRLGWHSVFAPALLGEPARRWHAAPGDGTDPGGPAYGIGLL
                     SRHPLLCPERTTLCGGGAGERRPEDAGRALPGWDHEPRTVLRAQVVPPGDRPLVVATA
                     HLSYLVWRGVRQLHHAASWTAACGLPALLIGDLNLPLRLLRPALAGTGWLVAAATATF
                     PAWRPGMQLDHLLGRSTRLRAVRVGPRGPSDHLPVSAQVVLA"
     gene            732..1469
                     /locus_tag="VFE39_11815"
     CDS             732..1469
                     /locus_tag="VFE39_11815"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET6259328.1"
                     /translation="MRRSGLRWSVIGTLLLAMLALLAAGRATVIMIYLGVAYRDEVDP
                     ISQPLSFYEFVDGGSQLLTSAVVALAVGALAVLFGMARAGVRLAGRPTVLFTTWALCL
                     VLAAIFPTDDSPRILTFAGWVHQFAGAGILALLSFAGFAAAPRLAESKPWRPVVGLVG
                     ALSVAAAALAVVYVVSRLNDWIPGFVSVFGGVDPGGILQRTALVFQGAVVAALAVQLA
                     RVSWPAVRPRTGPWLPDSTAPSPTPPC"
     gene            complement(1529..3268)
                     /gene="cobT"
                     /locus_tag="VFE39_11820"
     CDS             complement(1529..3268)
                     /gene="cobT"
                     /locus_tag="VFE39_11820"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HET6259329.1"
                     /translation="MNSDSFYEVVNGRRDVRSGFLPDAPVDDAVLRRVLAAAHAAPSV
                     GFSQPWDFLVIRDRAVRERVQAHVAASRAAYAASLPGARAAAFRDLKIEAVLDASLNI
                     AVTCDPTRGGRHTLGRQTQPRTASYSTVGAVQNLWLAARAEGLGVGWVSFGDERAMAA
                     LLGLPPHLELVAYLCVGPVDAFATEPELATVGWARRRPLRWAVHHDTYGRRGLPGEEP
                     MDLLDETVAAIVAPDAAARAAAGERLDRMTKPRGSLGRVEDVAVTLAGISGSCPPPVP
                     EPAAVAVFAADHGVHAQGVTPWPQEVTAQMVGNFLAGGAVVNAFARRLGAEVCVVDVG
                     VAAPLPNAGIGAVPGLLPRRVADGTADMTGGPALTRAQAQSAVEVGIETARDLVAAGN
                     RCLVTGDMGIANTTAAAALICVFTGADPAAATGRGAGIDDATLARKTAVVAAALRRHE
                     PDPADPVGVLAAFGGLEHAALAGFVLGAAALRVPVLLDGVNAGAAALVAAALAPAATG
                     YCIAGHRSAEPGHSHALAHLGLQPLLDLGMRLGEGTGALLALPVLQAAVQALLDVATF
                     DAAGVTEKAETQE"
     gene            complement(3335..4075)
                     /gene="cobI"
                     /locus_tag="VFE39_11825"
     CDS             complement(3335..4075)
                     /gene="cobI"
                     /locus_tag="VFE39_11825"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HET6259330.1"
                     /translation="MINTLVGVGVGPGDPELVTVKAANLLAKADIVFVPVADSGETGR
                     AEQTVLFYAEAWRVERVEFALHDREHTERRERAWDAAAAQVARWYARRPGGTAVFATI
                     GDPCVYSTFSYLAASVRDVVGELSVELVPGITAMQALAARSGTSLVEGREALALFPMT
                     AGPERFREALQRGDAVAAYKFGRMLPETLAVLRETGRIDEAVYGAGLGLAEEDIRPAC
                     ELDPREPGPYLSTLVVPPRRTGRGHGLR"
     gene            complement(4072..7932)
                     /gene="cobN"
                     /locus_tag="VFE39_11830"
     CDS             complement(4072..7932)
                     /gene="cobN"
                     /locus_tag="VFE39_11830"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051116 - cobaltochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HET6259331.1"
                     /translation="MTGAETPVLLLSTADTELLAARSSGGLYRTANPARVEPAGLPAL
                     LDGVSMVVLRLLGGRRAWPEGVDVLVACGLPVVLLGGEAAPDAELMAASTVPAGVASE
                     ALAYLAAGGRDNLRELHHFLSDTVLLTGEGFAPPAPAPDFGVHGEREQRPDRPTVGVV
                     FYRAHELSGNTAFVDTLCDAIEARGANALPVFCGSLRGLPPGASDEGSGLMELLARVD
                     ALVATVLAAGGTRATDAVAGGEEDSWDAGALAALDVPVLQGLCLTTSRATWAASDAGL
                     SPMDAAMQVAIPEFDGRLITVPFSFKELDAGDDIPVYRADPERAARLAGLAVRHARLR
                     STPVAERKLAVVLSSYPTKHARVGNAVGLDTPASAVVLLRALRDAGYAVGDFPEDSDT
                     LIHTLIAAGGHDVEWLTEEQLAAAPSRVRLTDYRRWFDALPAALTDGIREHWGEPPGS
                     LYVAGAGDDTAIVLASLRFGNVLLMIQPPRGFGENPVAIYHDPDLPPSHHYLAAYRWL
                     EHEFGADAVIHLGKHGTLEWLPGKGLGLSAECAPDAVLGELPLVYPFIVNDPGEGTQA
                     KRRAHAVVVDHLVPPMARADTYGEMAKLEQLLDEYATVAAMDPAKLPALRARIWEVVT
                     TAQLHHDLHLDSAPGEQDFDDFVLHIDGYLCEVKDVQIRDGLHVLGGVPAGEAQVNLV
                     LSILRATQVWGGRRALPGLRAALATWAGLDEQTLLATAGARVAGLDERPVRADVSAGS
                     AVGSTGGVQGEAGPGLTGLAPGPDERAVGADLLDDSAVRPVGEPLQRLVGLAGGPAVT
                     GADLVDVLEAVARALVTEVQTRGWSGAAAVCAELLGEEIPDVVAVLEFGCAEVVPRLE
                     RTTDEVAGVLHALDGGYVPAGPSGSPTRGLVNVLPTGRNFYSVDPKAIPSRSAWEIGS
                     ALAESLVARHLADTGTYPQSVGLTVWGTSAMRTQGDDLAEILALIGCRPVWDEASRRV
                     TGVEVVPFEELGRPRIDVTVRISGFFRDAFPHVITLLDDAIRMVAELDEPAEQNHLRA
                     HVEEDVAAHGDRRRATSRIFGSKPGAYGAGLLPLIDARNWRSDADLAEVYAVWGGYAY
                     GRGLDGREARADMEQSFRRIAVAAKNQDTREHDILDSDDYFQYHGGMVAMVRALTGRS
                     PAAYVGDSAVTDAVRTRTLAEETKRVFRARVVNPKWIAAMQRHGYKGAFELAATVDYL
                     FGYDATAGVVDDWMYSQLAESYVFDETNRAFMERSNPWALRGITERLLEAADRGMWAK
                     PDDAVLDRLRQTYLDLEGDLEGES"
     regulatory      complement(7963..8164)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(8241..8831)
                     /locus_tag="VFE39_11835"
     CDS             complement(8241..8831)
                     /locus_tag="VFE39_11835"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HET6259332.1"
                     /translation="MPNNEKSLLDLTVRELFDSVAARTSAPGGGGAAALVTALAAALT
                     GMASRFGDGAAVAEADDLRGRATPLADADAAAYQAFLDAVRLPHDDPSRPAAVATARR
                     DAIGVPTEIGELAAAVAELAAGLARDGNPRLRGDAVAAVRLAAAAAATAAELAAVNVH
                     GAEGTADLERARTAATAARHRVTELDREQPGPLTTG"
     gene            complement(8849..10069)
                     /gene="cobA"
                     /locus_tag="VFE39_11840"
     CDS             complement(8849..10069)
                     /gene="cobA"
                     /locus_tag="VFE39_11840"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HET6259333.1"
                     /translation="MTEPYLVGLDLAGRRVVVVGGGTVAQRRIAGLLAAGADVEVVAP
                     EVTTAVEGMATAQELHWTARGYADGDLVGAWYVVACTDDAGVNAAVAAEAERGHVFCV
                     RADDAPAGSAVTPAVGRHDGLTVGVLAGRRPRRSAAVRTALVEALQTGLVDDSAEPVR
                     PGVALVGGGPGDPELITVRGRRLLARADVVVTDRLGPRELLDTLAPHVEVIDASKIPY
                     GRAMNQARINELLIEHARAGRFVVRLKGGDPYVFGRGFEEVLACVDAGVPVTVVPGVT
                     SAFAAPAVADVPVSHRGVAHEIVVVSGHLAPGDPGSLVDWPALGRLRGTVVLLMAVER
                     IAAFAAALIDGGRPADTPVAVVQDGTMRIQRSVRATLATVADAVREHGISPPAVVVIG
                     PVAGLAAGPVTQLT"
     gene            complement(10066..>10696)
                     /locus_tag="VFE39_11845"
     CDS             complement(10066..>10696)
                     /locus_tag="VFE39_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010306248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HET6259334.1"
                     /translation="FGRPLGPHPVLQDLLDQRVTAALAGDPAAGTAVLLVGRGSTDPD
                     ANAEVCKVARLLQEGRAYDFVEPSFVSLARPDVITGLARCRALGARRVVVAPYFLFDG
                     VLPRRVVEQAVAFRAEHPDVDVRVADHLGDCDELAGLVVERYREALHGDIRMNCDTCA
                     YRVLLPGFEDKLGAPQTPHHHPDDPAHGHGHGHGGHPAATVDAVVGEWS"
BASE COUNT         1406 a         4077 c         3846 g         1367 t
ORIGIN      
        1 cgaccttgcc ggcaggctcg gctggcacag cgtgttcgcg cccgccctgc tcggcgagcc
       61 ggccaggcgg tggcacgccg ccccgggcga tggcacggac cccggcggtc ccgcgtacgg
      121 gatcgggctg ctgagcaggc atccgctgct ctgccctgag cgcaccacgc tctgcggggg
      181 cggcgcgggc gagcgacgtc ccgaggacgc cggacgcgcg ctgccaggct gggaccacga
      241 accacggacg gtgctgcggg cgcaggtggt tccaccgggc gaccggcccc tcgtcgtcgc
      301 caccgcgcac ctgtcctacc tggtctggcg cggcgtgcga cagctgcacc acgcagcgtc
      361 ctggaccgcg gcctgcggcc tacccgcgtt gctgatcggc gacctcaacc tgccgctgcg
      421 gctcctccgg cccgcacttg ccggaaccgg ctggctcgtg gcggctgcca ccgccacgtt
      481 cccggcgtgg cgaccgggca tgcagctcga ccatctgctc ggccgtagca cacggctgcg
      541 cgctgtgcgg gtgggtccgc gtgggcccag cgaccacctg cccgtcagcg cccaggtcgt
      601 cctcgcgtga ttcccgtctg accgtcacct cccggggtgg ccgtcggagg acgaccgaac
      661 ggtgaaaaag aaccgtttcg agtcgtcctc ggtgatcgcc tgcccccggc gggcgaggca
      721 tgatgtggac catgcggaga tccggcctgc ggtggtcggt gatcggcacg ttgctgctgg
      781 cgatgctcgc tcttctcgcc gccgggcggg ccacggtgat catgatctac ctcggcgtgg
      841 cctaccggga cgaggtcgac ccgatctcgc aaccgctgag cttctacgag ttcgtcgatg
      901 gtggctcaca gctgctcacc tcggcagtcg tggccctggc cgtcggagcc ctcgccgtgt
      961 tgttcgggat ggcccgcgcc ggggtgcggc tggcgggccg accgacggtc ctgttcacga
     1021 cgtgggccct gtgtctcgtg ctggccgcga tctttccgac cgacgactcg ccgcggatcc
     1081 tcacgttcgc cggctgggtg caccagttcg ccggggccgg gatcctcgcg ttgctgtcct
     1141 tcgccgggtt cgcggccgcc ccccgactgg ccgagagcaa gccgtggcga ccggtggtcg
     1201 ggctcgtggg ggcgctgtcg gtcgcggcgg ccgcgctggc ggtcgtgtac gtcgtgagca
     1261 ggctgaacga ctggattccc gggttcgtct cggtgttcgg cggcgtcgac cccggcggga
     1321 tcctgcagcg gacggcgctg gtgttccagg gcgcggtcgt cgcggcgctg gccgtccaac
     1381 tggcgcgggt gtcctggcct gccgtgcgcc cccgcaccgg cccgtggctc cccgactcca
     1441 cggcgccctc gccgacgccg ccgtgctgac ccaggtcagg gcgccgtcgt cccggccgcc
     1501 aggcccgcgt gacgtccggc agaaccgttc actcctgcgt ctccgccttc tcggtcaccc
     1561 cggccgcgtc gaacgtggcg acgtccagta gcgcctggac ggcggcctgc aacaccggga
     1621 gggcgagcag cgcgccggtg ccctctccca gccgcatccc gaggtccagc agcggttgca
     1681 ggccgaggtg tgcgagcgcg tggctgtgcc ccggttcggc ggagcggtgc ccggcgatgc
     1741 agtaacccgt cgctgccggg gcgagcgcgg ccgccaccaa tgcagcggcc cccgcgttca
     1801 ccccgtccag gagcaccggc acccgcagcg cagcggcacc gagcacgaac ccggccagcg
     1861 cggcgtgctc gagcccgccg aacgcggcga gcacgccgac cgggtcggcc gggtcgggct
     1921 catgccgcct gagcgccgca gcgacgaccg cggtctttcg ggccagcgtg gcgtcgtcga
     1981 tgccggcgcc cctgcccgtg gccgcagccg ggtcggcccc ggtgaacaca cagatcaggg
     2041 cagcggccgc ggtggtgttg gcgatgccca tgtcgccggt gacgaggcac cggttgcctg
     2101 ccgcgacgag gtcacgcgcg gtctcgatgc ccacctcgac ggccgactgc gcctgcgcgc
     2161 gggtgagtgc cggtccaccg gtcatgtccg cggtgccgtc cgcgacgcgc cgcggaagca
     2221 ggccgggaac cgctccgatg ccggcgttgg ggagcggcgc cgccaccccg acgtccacga
     2281 cacacacctc cgccccgagc cgccgcgcga aggcgttcac cactgccccg ccggccagga
     2341 agttccccac catctgggcc gtgacttcct gcggccacgg cgtcaccccc tgcgcgtgca
     2401 cgccgtggtc ggcggcgaac acggccaccg cggcgggctc gggcaccggt ggggggcagc
     2461 tcccgctgat cccggcaagc gtcacggcta cgtcctcgac gcgcccgagc gagccacgcg
     2521 gcttcgtcat ccggtcgagg cgctccccgg cggcggcgcg cgccgcggcg tccggcgcga
     2581 cgatcgcggc cacggtctcg tcgagcaggt ccatcggttc ctccccgggt agcccccggc
     2641 ggccgtacgt gtcgtggtgc acggcccagc gcagcggcct gcgccgcgcc caccccaccg
     2701 tcgccagctc cggctcggtc gcgaacgcgt cgacgggccc gacgcacagg tatgccacca
     2761 gctccagatg cggcgggaga ccgagtaggg cggccatggc gcgctcgtca ccgaagctga
     2821 cccagccgac gcccagaccc tccgcccgcg ccgccagcca caggttctgc acggcaccga
     2881 ccgtggagta ggacgccgtc cgcggctgcg tctggcggcc gagcgtgtgt cgaccgcccc
     2941 gagtcgggtc gcaggtgacg gcgatgttca gcgaggcgtc gagaacggcc tcgatcttca
     3001 ggtcgcggaa cgccgcggcc cgcgcgcctg gcagcgaggc tgcgtacgcg gcgcgggagg
     3061 cggcgacgtg ggcctgcacc cgctcccgca ccgcccggtc ccggatgacg aggaagtccc
     3121 acggctggga gaaccccacg ctcggcgcgg cgtgcgcagc ggcgagcacg cggcgcagca
     3181 cggcgtcgtc gacgggggcg tccgggagga aacccgagcg gacgtcccgc ctgccgttca
     3241 cgacctcgta gaaggagtcg gagttcacct ggccacctcc agcggtcgtg cgcggatacg
     3301 agtgtcatag cacccgtttc cgcgcacggg cgcgtcagcg caggccatgg ccgcgtcccg
     3361 tgcgccgggg cgggacgacg agggtggaga gatagggccc aggctcacga gggtccagct
     3421 cgcaggccgg acggatgtcc tcctccgcga gccccagccc cgcgccgtac accgcctcgt
     3481 cgatccggcc ggtctcgcgg aggacggcaa gggtctcggg cagcatccga ccgaacttat
     3541 aggcggcgac ggcgtccccg cgctgcagcg cctcacggaa ccgctccggg ccggccgtca
     3601 tcgggaacag tgcgagcgcc tcgcgcccct cgaccagtga cgtgccgctg cgcgcggcca
     3661 gggcctgcat cgcggtgatc cccggcacca gctcgaccga cagctccccc accacgtccc
     3721 gcacggacgc agcgaggtag ctgaacgtcg agtacacgca cgggtcgccg atcgtcgcga
     3781 agaccgcggt gccgcccggg cgccgggcgt accagcgcgc cacctgcgcc gcggccgcgt
     3841 cccaggcccg ctcccggcgt tcggtgtgct cgcggtcgtg gagcgcgaac tccacccgct
     3901 cgacccgcca cgcctcggcg tagaagagca cagtctgctc ggcgcgtccg gtctcgccgg
     3961 agtcggcgac gggcacgaac acgatgtcgg ccttggccag caggttcgcc gccttgacgg
     4021 tgaccagttc cgggtcgccg gggccgacgc cgacgccgac cagcgtgttg atcacgactc
     4081 gccctccagg tcgccttcga ggtcgaggta ggtctgccgc aaccggtcga gcacggcgtc
     4141 gtccggcttg gcccacatcc cgcggtcggc ggcttcgagc agccgctcgg tgatcccgcg
     4201 cagcgcccac gggttcgacc tctccatgaa ggcgcggttg gtctcgtcga aaacatagct
     4261 ctcggcgagc tgggagtaca tccagtcgtc gacgacaccg gccgttgcgt cgtagccgaa
     4321 caggtagtcg acggtggcgg ccagctcgaa cgcgcccttg tagccgtggc gctgcatcgc
     4381 cgcgatccac ttcgggttca ccaccctggc ccggaagacc cgtttggtct cctcggcgag
     4441 tgtgcgtgtc cgcaccgcgt cggtgaccgc cgagtcgccg acgtacgctg ccggcgagcg
     4501 cccggtgagc gcacgcacca tcgcgaccat cccgccgtgg tactggaagt agtcgtcgga
     4561 gtcgaggatg tcgtgctcgc gggtgtcctg gttcttggcc gccaccgcga tccggcggaa
     4621 cgactgctcc atgtccgccc gtgcctcacg gccgtccagc ccgcggccgt aggcgtagcc
     4681 gccccacacg gcgtagacct cggcgaggtc ggcgtcgctg cgccagttgc gggcgtcgat
     4741 cagcggcagc agcccggccc cgtaggcgcc cggcttggag ccgaagattc gagacgtggc
     4801 ccggcggcgg tcgccgtgcg cagcgacgtc ctcctcgacg tgggcgcgca ggtggttctg
     4861 ctcggcgggc tcgtccagct cggcgaccat ccggatggcg tcgtcgagca gcgtgatgac
     4921 gtgcgggaac gcgtcgcgga agaacccgga gatccgcacg gtcacgtcga tccgcggccg
     4981 gcccagctcc tcgaacggaa cgacctcgac gccggtcacc cgccgcgacg cctcgtccca
     5041 caccgggcgg cagccgatca gcgcgaggat ctcggcgagg tcgtcgccct gggtgcgcat
     5101 cgcggaggtc ccccacaccg tcaggccgac gctctgcggg tacgtacccg tgtcggcgag
     5161 gtgccgggcg accagcgact cggccagcgc gctcccgatc tcccacgcgg agcgcgacgg
     5221 gatcgccttg gggtccacgg agtagaagtt gcgcccggtc gggagcacgt tgaccaggcc
     5281 gcgggtcggc gagcccgacg gcccggccgg gacgtacccg ccatcgagag cgtgcagcac
     5341 accggcgacc tcgtcggtcg tgcgctccag gcgcggcacg acctcggcgc agccgaactc
     5401 cagcacggcc accacgtcgg ggatctcctc acccaggagc tccgcgcaca ccgccgcagc
     5461 accgctccag ccccgggtct gtacctccgt gacgagcgcg cgggcgaccg cttcgagcac
     5521 gtcgacgaga tcggcgccgg tgacggcagg ccccccggcc agcccgacga gccgctgaag
     5581 cggctccccc accgggcgaa cggcactgtc gtccagcagg tccgcaccga cggcccgctc
     5641 gtccggcccg ggcgcaagcc cggtcaggcc cggccccgcc tccccctgca cgcccccggt
     5701 tgaaccaacg gcacttccgg ccgacacgtc cgcccgaacg ggccgctcgt ccagtccggc
     5761 gacgcgcgcc cccgcggtgg cgaggagggt ctgctcgtcc agccccgccc aggttgccag
     5821 cgccgctcgg aggccgggca gggcgcggcg gccgccccag acctgtgtcg cgcgcaggat
     5881 cgacagcacc aggttgacct gcgcctcgcc ggccggcacc ccacccagca cgtgcagccc
     5941 gtcgcggatc tggacgtcct tgacctcgca caggtaaccg tcgatgtgca ggacgaagtc
     6001 gtcgaagtcc tgctccccgg gcgccgagtc gaggtgcagg tcgtggtgca gctgcgcggt
     6061 ggtgacgacc tcccagatcc gggcgcgtag cgccgggagc ttcgccgggt ccatcgcggc
     6121 gacggtggcg tactcgtcga gcaactgctc gagcttcgcc atctcgccgt aggtgtcggc
     6181 gcgcgccatc ggcggcacca gatggtcgac gacgaccgcg tgggcgcggc gcttggcctg
     6241 cgtgccctcg ccgggatcgt tcacgatgaa cgggtagacc agcggcagct cgccgagcac
     6301 ggcatcgggg gcgcactccg ccgacagccc caggcccttg ccgggcagcc actccagcgt
     6361 gccgtgcttg ccgaggtgga tcacggcgtc ggcgccgaac tcgtgctcga gccagcggta
     6421 cgcggcgaga tagtggtgcg acggcggcag gtccgggtcg tggtagatcg ccaccgggtt
     6481 ctcgccgaac ccgcgcggtg gctggatcat cagcagcacg ttgccgaacc gcagggacgc
     6541 cagcacgatg gccgtgtcat ctccggcgcc ggcgacgtag agcgagccgg gcggctcccc
     6601 ccagtgctcg cggatcccgt cggtcagggc ggccggcaac gcgtcgaacc accggcggta
     6661 gtcggtgagc cgcacccgcg acggagcggc cgcgagctgt tcctcggtga gccactcgac
     6721 gtcgtggccg ccggccgcga tcagcgtgtg gatcagcgtg tcggagtcct ccgggaagtc
     6781 ccccaccgcg tagccggcgt cccgcagcgc ccgcagcagc acgaccgcgg acgccggcgt
     6841 gtccaacccg accgcgttgc cgacccgtgc gtgcttcgtg gggtagctgg acaggaccac
     6901 ggccagcttc cgctcggcga cgggcgtcga ccgcagccgt gcgtgccgga cggcgagccc
     6961 ggccagccgg gcggcccgct cggggtcggc gcgatacacc gggatgtcgt cgcctgcgtc
     7021 gagctccttg aacgagaacg gcaccgtgat cagcctgccg tcgaactccg ggatcgccac
     7081 ctgcatcgcg gcgtccatcg gggagagccc cgcgtcggag gccgcccacg tcgctcggga
     7141 cgtcgtcagg cagagcccct gcagcacggg gacgtcgagc gcggcgagcg cgcccgcgtc
     7201 ccaggagtcc tcctcgcccc ccgctaccgc gtctgtcgcc cgggttccgc cggcggcgag
     7261 caccgtggcg accagcgcat cgacgcgggc cagcaactcc atcagaccag acccctcgtc
     7321 cgacgcaccc ggcgggagac cccgcagcga cccgcagaac accggcagcg cattggcgcc
     7381 ccgggcctct atcgcgtcgc agagcgtgtc gacgaacgcg gtgttgcccg acagctcgtg
     7441 cgcccggtag aagaccaccc cgaccgtcgg acggtccgga cgctgctcgc gctcgccatg
     7501 gacgccgaag tcgggtgccg gcgcgggtgg cgcgaagccc tccccggtga ggaggaccgt
     7561 gtcggacagg aaatggtgca gctcgcggag gttgtccctc ccccccgcgg cgaggtaggc
     7621 cagcgcctcg gacgccacac ccgcaggcac cgtcgacgcc gccatcagct ccgcgtccgg
     7681 cgcggcctcg ccaccgagca gcaccaccgg cagcccgcac gcgaccagca cgtccacgcc
     7741 ctcgggccag gcccggcgcc ccccgagtag ccgtagcacg accatcgaca ccccgtcgag
     7801 cagcgcgggc aggcccgccg gctcgacccg agcagggttg gccgtgcggt agagcccgcc
     7861 ggagctgcgg gcggccagca gctccgtgtc ggcggtggac agcagcagga ccggagtctc
     7921 ggcgcccgtc atcgttcctc ctcgagggtc cgcgccctct gttcggggac atgcgacggc
     7981 ggcgagtctc ctggctccca gatcagcacg ggccgcccga ccttcccgcc tcccggcagt
     8041 ggtcatgagg tgggcgtccg ctccccggtg acagtggcgg gaccgcgccg gattcccacc
     8101 ggcttcctcc actgccgtcg atggaaacca gatcacagag cccaccgccg ggtcaacgag
     8161 aggtctggca ccgaacgaac ccggacgagc ggcactgccg tccgatcggc ccggacgaac
     8221 gtgccgttcc agcgattggg tcagccggtg gtcagcgggc ccggctgctc ccggtccagc
     8281 tccgtcacgc ggtggcgcgc cgccgtggcg gccgtgcggg cgcgctccag atcggccgtg
     8341 ccctccgcgc cgtggacgtt gactgcggcc agctcggccg cagtggctgc ggccgctgcg
     8401 gcgagccgga cagcggccac ggcgtcgccg cgcagccgcg ggttgccgtc ccgggcgagg
     8461 ccggcggcga gctccgcgac ggccgcggca agctccccga tctccgtggg caccccgatc
     8521 gcgtcccgcc gggcggtggc gaccgcggcc ggccgggacg ggtcgtcgtg cggcagccgc
     8581 acagcgtcga ggaaggcctg gtaggccgcg gcatccgcgt cggccagcgg ggtggcgcgg
     8641 ccacggaggt cgtcggcctc ggcgaccgca gctccgtcgc cgaatcggga cgccatcccg
     8701 gtgagtgccg ctgccagcgc cgtgaccagc gccgccgcgc cgccgccgcc gggggccgag
     8761 gtgcgggctg ccacggagtc gaacagctcg cgaaccgtca ggtcgagcag cgacttctca
     8821 ttgttcggca cgatcgggtt cctcctgatc aggtcagctg ggtgaccgga ccagcggcca
     8881 ggcccgcgac cgggccgatc acgaccaccg cgggcgggct gatgccgtgc tcgcgcactg
     8941 cgtcggccac ggtggcgagg gtcgcgcgga cagaacgctg aatccgcatg gtgccgtcct
     9001 gcaccaccgc gacgggggtg tcggccgggc ggccgccgtc gatcagcgcc gctgcgaacg
     9061 cggcgatccg ctccaccgcc atcagcagga ccacggtgcc gcgcaaccgc ccgagcgcgg
     9121 gccagtcgac gaggctgccc gggtcgcccg gagccagatg tcccgaaacc acgacgatct
     9181 cgtgcgccac accccggtgg ctcaccggca cgtcggcgac ggcgggcgcg gcaaacgcgc
     9241 tggtcactcc gggcacgacg gtgacgggga cgcccgcgtc gacacacgcg agcacctcct
     9301 cgaacccgcg cccgaagacg tacgggtcgc cgcccttgag gcgcaccacg aaccgcccgg
     9361 cccgcgcgtg ctcgatgagc agctcgttga tccgcgcctg gttcatcgcg cggccgtagg
     9421 ggatcttgga cgcgtcgatc acctcgacgt gcggggcgag cgtgtcgagc agctcgcgcg
     9481 gaccgagccg atcggtgacg acgacatcgg cgcgggccag cagccgccgc ccgcgcaccg
     9541 tgatcagctc cgggtcaccc ggcccgccac cgaccagtgc caccccgggc cggaccggtt
     9601 cggcggagtc gtcgacgagc ccggtctgca gcgcctccac gagtgcggtg cggaccgcgg
     9661 ccgagcggcg gggacgccgc ccggccagta ccccgacggt gagcccgtcg tgcctgccga
     9721 cggcgggggt gacggcgctg ccggcgggag cgtcgtcggc gcgcacgcag aacacgtggc
     9781 cccgctccgc ctcggcggcg accgccgcgt tcacgccggc gtcgtcggtg caggccacca
     9841 cgtaccaggc cccaacgagg tcaccgtcgg catatccgcg ggcggtccag tgcagctcct
     9901 gcgccgtcgc catgccctcg accgccgtgg tgacctcggg cgccaccacc tcgacgtccg
     9961 caccggcagc gaggagcccc gcgatccgcc gctgcgccac cgtgccgccg ccgacgacga
    10021 cgacacggcg cccggcgagg tcgaggccga cgagatatgg ctcggtcatg accactcccc
    10081 caccaccgcg tcgacggtgg ccgccgggtg ccccccgtgg ccgtgcccgt gcccgtgcgc
    10141 ggggtcgtcg ggatggtggt gcggggtctg cggagccccg agcttgtcct cgaaccccgg
    10201 cagcagcaca cggtaggcgc aggtgtcgca gttcatcctg atgtcgccgt gcagcgcctc
    10261 gcggtagcgc tcgacgacga gccccgcgag ctcgtcgcag tcgccgaggt ggtccgcgac
    10321 acggacgtcg acgtccgggt gctcggcgcg gaacgccacg gcctgctcga cgacccgccg
    10381 cggcagcacg ccgtcgaaca ggaagtacgg cgcgacgacc acccggcgcg cgcccagcgc
    10441 gcggcagcgg gcgagcccgg tgatgacgtc cgggcgggcc agcgagacga acgacggctc
    10501 gacgaagtcg tacgccctgc cttcctgcag cagccgcgcg accttgcaga cctccgcgtt
    10561 cgcgtccggg tcggtggatc cgcggccgac cagcaacacg gccgtacccg cggccggatc
    10621 gccggccagt gccgcggtga cccgctggtc cagcaggtcc tgcagcaccg ggtgcggacc
    10681 gagcgggcgg ccgaac
//
LOCUS       DASRSC010000244         2570 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2223932,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000244 DASRSC010000000
VERSION     DASRSC010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2223932"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VFE39_11850"
     CDS             <1..1078
                     /locus_tag="VFE39_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6259335.1"
                     /translation="YDDQRALLADPRISADTIHPHYPFVSAGQAASRKQVQAFISMDD
                     PEHGRLRRMVNAPFAIKRVEALRPAIQKIVDGLIDDMLAGSTPVDLVEAFALPVPSLV
                     ICELLGVPYADHDFFQANSRILVKRTATPEQALAAQQRLLDYLDRLISEKLANPGNDL
                     LSELAVQHVATGELSWHEAASMARLLLIAGHETTANMIALGTLALLQHPEQLAELRAT
                     DDPKLIAGAVEELLRHLSIVHTGRRRVALTDIEIGGRTIRAGEGVILANDIGNRDPDA
                     FHDPDPDRLDLHRDARRHVAVGFGVHQGLGQPLARVELQVVYGTLYRRVPTLRLATGL
                     GDLDQIPFKHDGLVYGVYRLPVTW"
     gene            1105..1299
                     /locus_tag="VFE39_11855"
     CDS             1105..1299
                     /locus_tag="VFE39_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET6259336.1"
                     /translation="MKVTVDQNKCVASGQCVLAAPDIFDQRDEDGIVVLLDPDPPVDR
                     ADDVRLAAAVCPALAITVED"
     gene            complement(1746..>2570)
                     /locus_tag="VFE39_11860"
     CDS             complement(1746..>2570)
                     /locus_tag="VFE39_11860"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6259337.1"
                     /translation="GHVVLYLRGLLELVRGRYADALAAFRGAEPLARRLVAPHYTVPR
                     ARALLVHALVRLDDIDGAEQALAQLGSEDHEHGEMRIATAVLRLAQDDPHAAVAALAP
                     VVDGTSAALPWIGLAHAFLLEAIARDAIGDEDAADRALERALDLVEPGGALALFLLHP
                     APGLLERHPRHRTAHAALLTEILNLLAGNRRLPSRGGREPLREPLSASETRVLRYLPT
                     NLSAPEIADELYLSVHTVKTHMRHLYAKLDTHRRAEAVERARALYLLAPSSRERQD"
BASE COUNT          394 a          908 c          897 g          371 t
ORIGIN      
        1 ctacgacgac cagcgagcct tgctcgccga cccgcggatc agcgccgaca ccatccaccc
       61 gcactacccc tttgtcagcg caggccaagc agcgtcccgg aagcaggtgc aggccttcat
      121 cagcatggac gatccggagc atggccgcct gcgccggatg gtgaacgcac cgttcgcaat
      181 caagcgggtg gaggcgctgc ggccggccat ccagaagatc gtggacggcc tgatcgacga
      241 catgctcgcc ggctccaccc cagtggatct ggtcgaggcg ttcgccctgc cggtcccgtc
      301 gctggtgatc tgcgagctac tcggcgtccc gtatgccgac cacgacttct tccaggccaa
      361 ctccaggatc ctggtcaagc gcacggcgac tcccgagcag gcgctggcgg cccagcagcg
      421 gctgctcgac tacctcgacc ggctgatctc cgaaaagctc gcgaatccgg ggaacgatct
      481 gctctcggag ctggccgttc agcacgtcgc cactggggag ctgtcctggc acgaagccgc
      541 ctccatggct cgcctgctgc tgatcgccgg gcacgagacg accgcgaaca tgatcgcgct
      601 gggcacgctg gcgctgctgc agcaccccga gcagttggcc gagctgcgcg ccaccgacga
      661 cccgaaattg atcgcaggcg cagtcgaaga gctgctgcgc cacctgagca tcgtgcacac
      721 cgggcggcgc cgggtggcgc tgaccgacat cgagatcggc ggccggacca ttcgcgccgg
      781 cgagggcgtg atcctggcca acgacatcgg caaccgggac ccggacgcct tccacgaccc
      841 cgacccggac cggctcgacc tccaccgcga cgcccgccgc cacgtggccg tcggcttcgg
      901 tgtccaccag ggcctcggcc aacctctggc ccgggtcgag ctacaggttg tctacggcac
      961 cctctaccgc cgcgtcccta ccctgcggct ggccactggc ctgggggacc tcgaccagat
     1021 cccgttcaag cacgacgggc ttgtctacgg cgtctaccgc ctgcccgtca cctggtgacg
     1081 acaacgaatc agaggagaca tcccatgaag gtgaccgtcg accagaacaa gtgcgtcgcc
     1141 tccgggcagt gcgtgctcgc cgccccggac atcttcgacc agcgcgacga ggacggcatc
     1201 gtcgtcctgc tcgaccccga cccgcccgtc gaccgcgccg acgacgtccg gctcgcggcc
     1261 gcggtgtgcc ccgccctggc catcaccgtc gaggactaga cccgtgctcg atcgggtcgt
     1321 gatcgtgggg ggcctcggcc ccgggcctga ctggcccgcg cgtcgggctg ttgaccgccg
     1381 cgagtggtcg tggtcggcgc cggcttcctc ggcgccgagg tggccgcggt cccccggagc
     1441 atggctccca ggtgccggtt cgatcaggaa ccggagcacg acgtcgcgca cccccgcgtg
     1501 aatcaccgat cggctgtcca tcgttggaga tcttcggttg gctgatggct acccgggacg
     1561 ggcccgcgag cacgagcgga tgaccgctca ccatgaggcc cgacgtgtcc tgggccagcg
     1621 tcctgatcat gaccaggcgg tcgcccactg ccgccggaaa ccgcgatgcg gcggggaggg
     1681 gtgggcaagc cagcacgccg gccacgatcg ggcagcggca cgaatgccct tcgtcgacag
     1741 gctcgtcagt cctggcgctc acgcgaggag ggggcgagca ggtagagggc gcgggcccgt
     1801 tcgacggcct cggctcggcg gtgggtgtcg agcttggcgt agaggtggcg catgtgcgtc
     1861 ttgactgtgt gcacggacag gtagagctcg tcggcgatct ccggggctga gaggttggtc
     1921 ggcaggtagc gcaggacacg ggtctcgctg gcgctgagcg gctcccgcag cggttcccgc
     1981 ccgccgcgcg atggcagacg tctgttgccg gcgagcaggt tgaggatctc ggtgagcagg
     2041 gcggcgtggg cggtgcgatg ccgggggtgg cgctcgagca gccccggcgc ggggtgcagc
     2101 aggaacaacg cgagcgcgcc gccaggctcg acgaggtcga gcgcgcgctc cagtgcccga
     2161 tcggcggcat cctcgtcgcc gattgcgtcc cgggcaatcg cctccagcag gaacgcgtgc
     2221 gcaagcccga tccacgggag tgcggccgag gtaccgtcga cgaccggcgc aagcgcagct
     2281 accgccgcgt gcgggtcatc ttgggctagc cgcagcaccg cggtggcgat acgcatctcg
     2341 ccatgctcgt ggtcttcgct gccgagttgg gcaagggctt gctcggcgcc gtcgatatcg
     2401 tccaggcgca ccagagcgtg caccagcagc gctcgtgccc gaggcacggt gtagtgcggg
     2461 gcgacgagtc gacgggccag cggttcggcg ccccggaacg cggccagcgc atcggcgtac
     2521 cgcccgcgca ccagctcgag cagcccacgg aggtaaagga caacgtgtcc
//
LOCUS       DASRSC010000245        23272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5823465,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000245 DASRSC010000000
VERSION     DASRSC010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 23272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23272
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5823465"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1238
                     /locus_tag="VFE39_11865"
     CDS             <1..1238
                     /locus_tag="VFE39_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005437821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HET6259338.1"
                     /translation="IDPPGDTRSDLWFYFHLGRRIREKLAGSTDPRDRPILELTWDYP
                     TEGPYDEPSADAVLREINGADEDGKALSGYTGLKADGSTSSGCWIYSGVYAEEVNQAA
                     RRRPGHEQSWVAPEWGWAWPMNRRILYNRASADPDGNPWSERKALLRWDADEGKWVGP
                     DVPDFEVTKPPNYRPPDGAKAQDALAGDEPFIMQSDGRAWLHVPAGLVDGPLPAHYEP
                     EESPVRNALYGQQRNPARQRYHRPENQYHPDAGTPEADVFPFVFTTFRITEQHTAGGM
                     SRWTPYLNELQPAMFLEVSPELAAERGLEHTGWATVVTARSAIEGKVLVTERAKPLRV
                     GGKVVHQIGIPWHWGPNGLSTGDAANELLGVTLDPNVHIMETKAATCDIRPGRRPRGP
                     QLVEYLAAYRRRAGLEEHG"
     gene            1235..2152
                     /locus_tag="VFE39_11870"
     CDS             1235..2152
                     /locus_tag="VFE39_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HET6259339.1"
                     /translation="MTNRLSGPLPDPSADAGYPSDHPERVGFFTDTSVCIGCKACEVA
                     CKEWNALPSEEAGVPGDLTGMSYDNTGGLGASTWRHVAFVEQSVPARKSPQVDLGMPA
                     ATLPGAGTDGSGDATEVRWLMSSDVCKHCTHAACLDVCPTGALIRSEHGTVVVQNDIC
                     NGCGYCIPACPYGVIDQRPGDGGAFKCTMCHDRLGAGLMPACATACPTESIQFGPLDE
                     LRARAAERVDALHLAGVTDARLYGHDPNDGVGGDGAFFLLLDEPEVYGLPPDPVVTTR
                     DLPSMWRHAGAAAAAVAGVVAAAFLGARR"
     gene            2149..3309
                     /gene="nrfD"
                     /locus_tag="VFE39_11875"
     CDS             2149..3309
                     /gene="nrfD"
                     /locus_tag="VFE39_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HET6259340.1"
                     /translation="MRWRRSAERRNTSWTGDRVGGWAPYGTRDDRPGGTAPGGAGPGG
                     AGPDRSVAQNPRVQQRRRGRGEQLMVPDAEFRSYYDRPIIKPPVWKVPDVPTYLYLGG
                     VAGVSSATAALADATGRPVLRRVGRLAAAFGATVSVGALVHDLGRPTRFLHMLRVFKP
                     TSPLSVGSWILAPFGALAGATAASELTGIAPGLARLAGAGAGALGPALTTYTAVLLAD
                     TAVPAWHEAYRELPFVFAGSALASGGGVGMLAGHEAGPARAIAVAGAAVELAATSVLE
                     RRIGLAARAYRTGRAGAVLKAARAATVAGAVGAVLSAKAGRHTRLLSVVSAALLNAGS
                     AATRYGVFEAGMASARDPEYTVVPQRERLDARAQGVAVDRTADRSSPPAVST"
     gene            complement(3355..3669)
                     /locus_tag="VFE39_11880"
     CDS             complement(3355..3669)
                     /locus_tag="VFE39_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259341.1"
                     /translation="MAGGLAASAVGVAAQLPEATGTALLGVAREAFVTRLHLTSAAAA
                     VIAAVIAIIAAVALEQPAQLARTRHGNADDPPVGPGRMSDSPDIEEKVLSPCSGAVCE
                     TG"
     gene            complement(3682..3804)
                     /locus_tag="VFE39_11885"
     CDS             complement(3682..3804)
                     /locus_tag="VFE39_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259342.1"
                     /translation="MIDVGLFRIRAFNAALVVNFLAIFVMVGYFLFIGQYLQRA"
     gene            complement(3801..3944)
                     /locus_tag="VFE39_11890"
     CDS             complement(3801..3944)
                     /locus_tag="VFE39_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259343.1"
                     /translation="MSVVALLAVVYGLKQIAQDGVSVVPAVAGGLGVGALWVRRQRQL
                     ADA"
     gene            complement(4096..4656)
                     /locus_tag="VFE39_11895"
     CDS             complement(4096..4656)
                     /locus_tag="VFE39_11895"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259344.1"
                     /translation="MSRSSLSLACSVLAAGMVALTGCAASNTPAPAAPAVSPPTTAAA
                     GGATQQPGGHHGGHGSPGDAGPSLYAVQSGPLGVVATDGAGHLMYRSDADSADPSTSN
                     CSGSCAQTWLPVLVAAGQEPELLGVDAAAVGRLTRPEGTTQLTLAGWPLYRHRDDQGG
                     LQSAGQNGADGIWFAITPTGDKATAA"
     gene            complement(4714..6768)
                     /locus_tag="VFE39_11900"
     CDS             complement(4714..6768)
                     /locus_tag="VFE39_11900"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HET6259345.1"
                     /translation="MDFDSAYRHGFLRVAACTLSTSIADPAANAESVLRVARECHDEG
                     VGVAVFPELTLSGYSIEDILLQDSLLDAVEQALLDVVTGSADVLPVLVVGAPLRFRHR
                     VYNTAVVIHRGRILGVVPKSYLPNYREFYERRQLAPGDDISTTILIGGPDAALDVPFG
                     PDLLFSASDVPGFVLHVEVCEDMWVPIPPSAEATLAGATVLANLSGSPITVGRAEDRH
                     LMCRSASSRCLAAYVYAAAGEGESTTDLAWDGQTMVYENGVLLAESDRFPQGERRSVA
                     DVDLDLLRAERLRMGTFDDNRRHHSARTDSFRRVEFRLDPPGGDIGLLRELERFPFVP
                     ADESRLEQDCYEAYSIQVSGLEQRLRAIGQPKVVIGVSGGLDSTHALIVAAKAMDRQG
                     RPRSDILGFTLPGFATGERTKGNAIRLARALGITFEELDITATARLMLTEIGHPFSRG
                     EPAYDVTFENVQAGLRTDYLFRIANQRGGIVLGTGDLSELALGWSTYGVGDQMSHYNV
                     NGGVPKTLIQHLIRWVVSSKQFEAEVGEVLESVLDTEITPELVPTGEDEQVQSSEATV
                     GPYSLQDFSLFHVLRYGFRPSKIAFLAWHAWRDPDHGDWPAGFPADKRPAYTLAEIRH
                     WLEVFAQRFYSFSQFKRSALPNGPKVSAGGSLSPRGDWRAPSDMSARIWLEEVRREIP
                     EE"
     gene            6959..7141
                     /locus_tag="VFE39_11905"
     CDS             6959..7141
                     /locus_tag="VFE39_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259346.1"
                     /translation="MIVVIWLVIGLIATYQRGYFGQSDVNCAALGTVLVTVVVGPLNY
                     LGVNPRIECQVPQPSP"
     gene            complement(7220..7450)
                     /locus_tag="VFE39_11910"
     CDS             complement(7220..7450)
                     /locus_tag="VFE39_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015102448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259347.1"
                     /translation="MATAETGHITGTRDKDYNLIWFTEACLDNTLRLETYIEDAESDG
                     DNEVAELFRRAQTESRKGADQGKQLLARRLSG"
     gene            complement(7509..7661)
                     /locus_tag="VFE39_11915"
     CDS             complement(7509..7661)
                     /locus_tag="VFE39_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259348.1"
                     /translation="MPTLLSTMHDDLPDLRLLLVSGEACPQDLAIHWHRPFDVRRWVP
                     DLATGV"
     gene            complement(7806..9092)
                     /gene="ectB"
                     /locus_tag="VFE39_11920"
     CDS             complement(7806..9092)
                     /gene="ectB"
                     /locus_tag="VFE39_11920"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HET6259349.1"
                     /translation="MMSERPVSTGNEVFARRESRVRSYSRKYDVVFEEARGSLVWDTE
                     GREYLDFLAGAGALNYGHNDPDMADALVAHIRGGGITHGLDLFTRPKMEFLEAFEGLI
                     LEPRGMDYRVQFTGPTGTNAVEAALKLARKVTGRRNVIAFTNGFHGVSQGALAATGNR
                     DKRMGTTLSLPDVTRLPYDGYLGGSVDTADLLDRMLSDHSSGVDEPAAILLETVQGEG
                     GLNVASRPWLRRIAAIAKTYGALLIVDDIQAGCGRTGSFFSFEGTGVTPDLITMSKSI
                     SGFGLPMALLLLKPELDIWKPGEHNGTFRGNAHAFVTARVALEKFWGDRSFQEDVVAS
                     GVLLGERLTQIAKLVPGARVKGRGMMQGIDVRDGSVAAEICRLCARQQLIVETAGPDD
                     EIVKILAPLTTPTHLLHRGLDIIEESAAANLHDVAV"
     gene            9480..10223
                     /locus_tag="VFE39_11925"
     CDS             9480..10223
                     /locus_tag="VFE39_11925"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6259350.1"
                     /translation="MTDGSVAVVTGAGSGLGRVIARALLCASFRVALAGRRRDALEDT
                     AAGEPGAVALPTDVADAASVARLFGAVRETWGRVDLLVNNAGTSGPSGSIDEIAVEDW
                     TATVGTNLTGAFLCAREAFAAMRAQQPQGGRIINNGSISAHTPRPGSAAYTATKHAIT
                     GLTRSISLDGRPYRIACGQIDIGNAATDMTARIATGVRQADGSVRPEPTFDAAHVADA
                     VLLMARLPLDTNVQFLTITATSMPFIGRG"
     gene            complement(10276..11049)
                     /locus_tag="VFE39_11930"
     CDS             complement(10276..11049)
                     /locus_tag="VFE39_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6259351.1"
                     /translation="MDTGLQGRTAIVPGSSSGLGLAVARTLAAEGANVVLAGRRGELV
                     RAEAAKLPSAVGVEADLADPDAPATLVAEAEQRFGQVDVLVLNGGGPPPGTAAEFTPE
                     QFAAAVNLLVQPHLRLVAAVLPGMRERGFGRIVAVGSSGVQQPIEGLVASNAARAALA
                     GYLKTLAGEVAADGVTVNMVLPGRIETDRVAVLDRAIAERSGTTPEQQRARAEAAIPA
                     RRYGTPEEFAAVVTFLASAAASYVTGEQVRCDGGMIRTH"
     gene            11327..12010
                     /locus_tag="VFE39_11935"
     CDS             11327..12010
                     /locus_tag="VFE39_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HET6259352.1"
                     /translation="MPVTDEAIEKIKAMIVSGELGPGARLPREADLAERLGLSRNSLR
                     EAVKALSLIRVLDVRQGDGTYVTSLDPHLLLDAMTFVVDFHRDDTVLEFFEVRRILEP
                     AATAMAALTMTEEQVDGLRAVLAELDQDPGVEALVANDLEFHRRVAVGSGNTVLCSLI
                     EGLSGPTTRARIWRGLTQEGAVARTIEQHAAICDAIAARQPDIARSWATIHVAGVEQW
                     LRSALGDSP"
     gene            complement(12133..12903)
                     /locus_tag="VFE39_11940"
     CDS             complement(12133..12903)
                     /locus_tag="VFE39_11940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6259353.1"
                     /translation="MTAGGGGEFAGLAAIVTGGGSGIGLATARLLAERGARVACLDLD
                     PSGVDAPLLGLATDVTDQDSVDAAVATVVERFGGLDVLVNNAGIGAQGTVETNPLDEW
                     HRVLDVNVLGIVRLSRSALPHLRRSSAAAIVNTCSIAATAGLPQRALYSASKGAVQSL
                     TLAMAADHVAEGIRVNCVNPGTADTPWVGRLLDAASDPAAERAALEARQPMGRLVSAP
                     EVAAAIAYLASPVSAATTGTALFVDGGMQGLRLRPRQD"
     gene            complement(13288..14259)
                     /locus_tag="VFE39_11945"
     CDS             complement(13288..14259)
                     /locus_tag="VFE39_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HET6259354.1"
                     /translation="MDLFVGMDVGGSRSRAAVVDGAGRVCGAAEGPGGNPVSHPPDQA
                     FRALAGAMRAVLQGLPASAVRAAVIGMAGVGSVRLPGVSASLADLAVEVGLRCRPTVV
                     GDVVVAFAAGTPEREGTVLISGTGATAARIAGLEPVDGTDGHGWLVGDVGSAFWLGRR
                     AVRAALAALDGRRTPTALLPAVTAALLGAAVNTPDSGVTAEQIVHTVHAAPPIALARL
                     APLVSSAARAGDAVAGTIVAAAATALVADVHAIRHPDERTPIVLAGSVAGGDTPVAEL
                     LRTRLSARWPGCVTTAGDTARAAAWLAAARSVPAPRAADLHAVILRT"
     gene            14360..15319
                     /locus_tag="VFE39_11950"
     CDS             14360..15319
                     /locus_tag="VFE39_11950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010307918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET6259355.1"
                     /translation="MAQVAGSDTPAEAGTGQLLVHLRSLLPGLAGALRQVANVILEDP
                     AWASRATIVELGERSGTSPATVTRFCRTLGVAGYTELRVGIATEIGRADQAGWEVGIG
                     ADVLPTDPLDRVLRTLLAVDMQAMHETAAALDLAVIDAVADAVVAARRVDFYATGGSA
                     AVLEDQQLRLYRIGIATWTWADVHNGLTSAALLGAGDVAIALSHSGRTTETVEMLAEA
                     GRRGATTVAITNFSTSPIADVADHLLGTSVRETAFRAGAMSARHSQFLVLDLLYLAVA
                     QRTHERSTEAFARTADAVAGHRTPYEPRARGGSARSVDPHRGA"
     gene            15319..16110
                     /locus_tag="VFE39_11955"
     CDS             15319..16110
                     /locus_tag="VFE39_11955"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET6259356.1"
                     /translation="MTSERHTTSEAAASDPVSAAAFAEAAAVAVRRVSTEQADAVAAA
                     AALLVDGLRAGGVVQTFGTGHSQALAMEIAGRAGGLVPTNMIALRDLVILGGDRPDVL
                     FNEHLERDPAVAHRIYELAGVHPSDVFVMASNSGGNGSVVELARIVKERGHGLVAITS
                     MAHSSRVTSRHPSGLRLFELADVVLDNGAPYGDAVLTTGELAVGGISSITAALLAQMV
                     VAEVVRTMLAAGEAPPVYLSANIPEGDAHNSALEARYAGRIRRSA"
     gene            16225..17652
                     /gene="ngcE"
                     /locus_tag="VFE39_11960"
     CDS             16225..17652
                     /gene="ngcE"
                     /locus_tag="VFE39_11960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_function: GO:0048030 - disaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0006032 - chitin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0015764 - N-acetylglucosamine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine/diacetylchitobiose ABC
                     transporter substrate-binding protein"
                     /protein_id="HET6259357.1"
                     /translation="MNPSRPVLGLDRRQFLQRLAAAGLLAVPGAGLLSACATGGGGTT
                     TAAQGQVSATNPLGVATDAPLDVVIFKGGYGDDYALAHEAMYKEHYPQAQVKHSGSTQ
                     IAQEMQPRFVAGNPPDVLDDTGSQKIEYAVLVNDNQLTDLGPLLDAPSADDPNVKVRD
                     TLLPGVIEQGTFGDKFVILNYVYETYALWYSKPLFEKNGWQAPTTWDEMMALCEKIKA
                     AGIAPFTYGGVNASDYVIDLNLGAAIKQGGLDVVKKMDNLQPGAWTQDTVLAATQRLE
                     EMVRKGYFMPGSEGLKHTEAQTAWVQGKAAFYVSGSWIENEMKGITPPDFAMTAAPIP
                     VLGSGSAMPVAALRTQPAEAFVVPAKAKNVHGGMEYLRLMLSKQGGEKFAELTASVPA
                     VKGATANAKKTPALESLSAALAAAGSNVFSWQFRTWYSSYSTLLITETTNLLTGRTDA
                     AGYLAKLQAETDRIAADQSIAKYHR"
     gene            17659..18576
                     /locus_tag="VFE39_11965"
     CDS             17659..18576
                     /locus_tag="VFE39_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6259358.1"
                     /translation="MRHGKYRFIASFLAIPLVLYVVFVVSPYAQAFYISMTDWRGFSG
                     QQNFIGLRNYAELATDGRFWQAMGHNGLLILVVPLVTIAFALFLAAVTTVAGRQGAVG
                     RGGVRGSRFYQVVFFFPQVVPVVIAGVLFGFFFNPDAGLLNGVLRVVGLGWLTRPWLG
                     DPALALPSVMAVLIWGAVGFYYVLFSSAMKSVPPDVFEAAVLDGAGRLRTFWSITLPM
                     IWDSVQVSYIYLGIMVLDGFALLQVMLPVPGGGPDGSTVVVSQYLYETAFTGGRFGYA
                     SAIGVALCLVTLLLAVAVLRLTRRQQGEY"
     gene            18588..19502
                     /locus_tag="VFE39_11970"
     CDS             18588..19502
                     /locus_tag="VFE39_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6259359.1"
                     /translation="MTLETSAPATAPPARPQRRASRQDTAGRSTGERTLGVFTHGFLV
                     AWAILVAVPLLWAVLTAFKTDSEIFTNPWSLPAHWQFGNFARAWTTANIGTYFVNSLI
                     VVSVSVVLVMLLGSMVAYVLARYEFPGRSVIYYAFVAGMTFPIFLALVPLFFVVQNLG
                     MLGTYQGLILVYTAYALPFTVFFLTGFFRTLPGALAEAAMLDGCSHAGVFFRIMLPLA
                     RPGLISVGIFNFLGLWNQYLLPLVLMPDSQHYVLSQGLAVLAANQGYRSDWSALFAGL
                     VIALLPVLAVYVIFQRRIQEGLSVGAIK"
     gene            19611..19847
                     /locus_tag="VFE39_11975"
     CDS             19611..19847
                     /locus_tag="VFE39_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259360.1"
                     /translation="MFGLPQPVKRVLAGRRVERDGQVLDLDLQLFGRIDVLLASSAGG
                     TVDQAALAEQRRQADLAAEVVAAAGLDDVVTEDL"
     gene            19848..20384
                     /locus_tag="VFE39_11980"
     CDS             19848..20384
                     /locus_tag="VFE39_11980"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HET6259361.1"
                     /translation="MPRGAGPLAARLAVPPAVPETAGLVYFHGGGFVMGSIASTDPLC
                     RLLEAQSGVRVLSVDYRLAPEDPYPAALEDAIAAYPGPGPTRRGAVRRHAILRADDLS
                     GMPPALVESKLTGRSTVDRPSSSSASPGHTERGRRGRCATRNRRVCVQVEPRPGATVT
                     GTRVDQSRSLDGRPDRRE"
     gene            20465..21166
                     /locus_tag="VFE39_11985"
     CDS             20465..21166
                     /locus_tag="VFE39_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HET6259362.1"
                     /translation="MGAGTEFLGRIVDVLPDDAFRAPSALPGWSRAHVVAHLARNAEA
                     LSRLANWARTGIETPMYADREQRAAEIESSAQAPVERLRRELVSTADELDAALAALDD
                     AAWRAEVRTALGRAIPATEVPWMRVREVWLHAVDLDAGGALTDLPLEIVDALLDEVTV
                     TLSSREGCPAARLAPLDRDRTWPLGPEPAERIVEVRGDAARVLGWLIGRSAGGVDAVD
                     AGGAPAAVPAAPRWL"
     gene            complement(21240..21689)
                     /locus_tag="VFE39_11990"
     CDS             complement(21240..21689)
                     /locus_tag="VFE39_11990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_6728 family protein"
                     /protein_id="HET6259363.1"
                     /translation="MQTFLPYPDFAASAAVLDNLRLGKQRVEAMQVLRAVTRATYGWK
                     RHPAVRMWAGYPEGVAAYGLVICAEWVGRGRADTCAATIGTDLAEHGLPPPRTQAELA
                     ALSLLPKWIGDERVHRSHRSALVRKDPEFYGPLFSDADPEAGYFWPV"
     gene            complement(21700..22299)
                     /locus_tag="VFE39_11995"
     CDS             complement(21700..22299)
                     /locus_tag="VFE39_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259364.1"
                     /translation="MTDRFDGWIAGLGTAAGLRVVVGCWPRSPLGAFTDVMVEQPDGH
                     RVLLAPSGEVADFVAATYVFDEVRVAPVHAEVDGARWRVDAAPLDLAFTVGARTPLGL
                     LLCAVPRPMATSPRWINAIDVVARRLLPGVRTTGSAVGGRREYYAALDLHGLTAASTT
                     WDGADQGVLAPVAPPVRFGFGSTPRAPSLTRVVTLVQQA"
     gene            22522..23043
                     /locus_tag="VFE39_12000"
     CDS             22522..23043
                     /locus_tag="VFE39_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6259365.1"
                     /translation="MPQPEGAELEAVRARRVALKDKYLRAVGERPATTVRGVHHLALI
                     ARDVEETIKFYQDLLGFPLVELVENRDYQGSSHFFFDIGNRNLLGFFDFPGHDHPDFS
                     ETIGGVQHLALSTSPEEFAAARKRLDDAGVDYLGPDRGVDNSLYIRDPNGVGIEFYCE
                     ELGKFEGEPLLSS"
BASE COUNT         3350 a         8184 c         8355 g         3383 t
ORIGIN      
        1 cgatcgaccc gcccggtgac acccgcagtg acctgtggtt ctacttccac ctgggccggc
       61 ggatccggga gaagctggcc ggctcgacgg acccgcggga ccgtccgatc ctagagctga
      121 cctgggacta tccgacggag ggtccctacg acgaaccgag cgccgacgca gtcctgcggg
      181 agatcaacgg cgccgacgag gacggcaagg cgctctccgg ttacacgggg ctcaaggccg
      241 acggatccac cagcagcggt tgctggatct acagcggggt gtacgccgag gaggtcaacc
      301 aggccgcgcg gcgcaggccc ggccacgagc agagctgggt ggccccggag tggggctggg
      361 cgtggccgat gaaccggcgg atcctgtaca accgggcgtc cgccgacccg gacggcaacc
      421 cgtggagcga gcgcaaggca ttgctccgct gggacgccga cgagggcaag tgggtcgggc
      481 cggacgtgcc cgacttcgag gtgacgaagc cgccgaacta ccggcccccg gatggtgcga
      541 aggcgcagga cgcgctcgcc ggcgacgagc cgttcatcat gcagtccgac ggccgcgcgt
      601 ggctgcacgt cccggccggc ctcgtcgacg ggccgctgcc cgcgcactat gagccggagg
      661 agtcgccggt gcgcaacgcg ctgtacgggc agcagcgcaa cccggcccgc cagcgctacc
      721 accgcccgga gaaccagtac cacccggatg cgggcacacc ggaggcggac gtcttcccct
      781 tcgtgttcac cacgttccgg atcaccgagc agcacacggc cggcgggatg agccggtgga
      841 cgccgtatct gaacgagctg cagcccgcga tgttcctcga ggtctctccg gagctcgccg
      901 ccgagcgcgg actggagcac acgggctggg ccacggtggt cacggcgcgg tcggcgatcg
      961 agggcaaggt gctggtcacc gaacgggcca aacccctacg ggtgggtggc aaggtcgtgc
     1021 accagatcgg tatcccgtgg cactggggcc cgaacggcct ctcgaccggg gacgcggcca
     1081 acgagctgct cggcgtcacg ctcgacccga acgtgcacat catggagacg aaggccgcca
     1141 cctgcgacat ccgccccggc cgacggccta gagggccgca gctggtggag tacctggccg
     1201 cgtaccggcg ccgcgccggg ctggaggagc acggatgacc aaccgcctct cggggccgct
     1261 gcccgacccg tcggccgatg ccggctatcc ctccgaccac ccggaacggg tcgggttctt
     1321 caccgacacc agcgtctgca tcgggtgcaa ggcctgcgag gtggcctgca aggagtggaa
     1381 cgcgctgccg tcagaggaag caggcgttcc cggcgacctc accggcatga gctacgacaa
     1441 caccggcggc ctcggggcga gcacctggcg gcacgtggcg ttcgtggagc agagcgtgcc
     1501 cgcgcggaag tcgccgcagg tcgatctggg gatgcccgct gccacgctgc ccggagccgg
     1561 gacggacggc agcggcgatg ccaccgaggt ccgctggctg atgtcctcgg acgtctgcaa
     1621 gcactgcacc cacgcggcct gcctggacgt ctgtcccacc ggggccttga tccgctccga
     1681 gcacggcacc gtggtcgtgc agaacgacat ctgcaacggc tgcggatact gcatcccggc
     1741 atgcccgtac ggcgtgatcg accagcggcc cggcgacggt ggcgcgttca aatgcacgat
     1801 gtgccacgac cgcctcggcg ccgggctcat gcctgcgtgc gccacggcgt gcccgacgga
     1861 gtcgatccag ttcggcccgc tggacgagct gcgtgcccgc gccgccgagc gggtggacgc
     1921 gctgcacctg gccggggtca ccgacgcgcg gctgtacggg cacgacccga acgacggcgt
     1981 cggcggcgac ggcgccttct tcctgctgct cgacgagccc gaggtgtacg gcctgccgcc
     2041 ggacccggtg gtcaccaccc gcgatctgcc ctccatgtgg cgccatgccg gtgccgcggc
     2101 cgccgcggtg gccggtgtcg tggcggcggc gttcctcggg gcgcggcgat gaggtggcga
     2161 cgttccgcgg aacgcaggaa cacgagttgg accggcgacc gcgtcggcgg ttgggcgccc
     2221 tacggcacca gggacgaccg gccgggcggc accgcgcccg gcggggccgg tccggggggc
     2281 gcgggcccgg accggtcggt ggcccagaac ccgcgggtcc agcagcgtcg acgtgggcgc
     2341 ggcgagcagt tgatggtgcc cgacgcggag ttccgctcct actacgaccg cccgatcatc
     2401 aagcccccgg tctggaaggt gcccgacgtc ccgacctacc tctacctcgg tggtgtggcg
     2461 ggggtgtcgt cggcgacggc cgcgctggcc gatgcgaccg gcaggccggt actgcgccgc
     2521 gtcgggcggc tggccgcagc gttcggggcc accgtcagtg tgggcgcgct ggtccacgac
     2581 ctgggcaggc ccacccgatt cctgcacatg cttcgggtgt tcaagccgac ctcgccactg
     2641 tcggtcgggt cgtggatcct cgcgccgttc ggcgctctgg ccggcgccac cgccgcctcc
     2701 gagctcacgg ggatcgcgcc gggcctcgcg cgcctcgccg gggcaggcgc cggtgccctg
     2761 ggcccggcgc tgaccacata cacggccgtg ctgctggccg acaccgcggt tccggcgtgg
     2821 cacgaggcct accgggaact cccgttcgtg ttcgccggca gcgcgctcgc cagcggtggg
     2881 ggcgtgggga tgttggcggg ccacgaggca ggcccggccc gcgccatcgc cgtggcgggg
     2941 gcggccgtcg agcttgccgc cacgtccgtg ctggaacggc ggatcggact ggccgcccgc
     3001 gcctaccgca ccggtcgtgc cggagccgtg ctgaaagccg cgcgagcggc caccgtcgct
     3061 ggagcggtcg gggcggtgct gtccgcgaag gcaggccggc acacgcgact gctctcggtg
     3121 gtgtccgccg cgctgctgaa cgcaggctcg gctgccaccc gttacggggt gttcgaggcc
     3181 ggcatggcgt cggcccgcga ccccgagtac acggtagtcc cgcagcgcga gcggctcgat
     3241 gctcgcgctc agggcgtcgc ggtcgatcgt acggctgacc gatcttcgcc gcccgcggtc
     3301 agcacatgat cagcagaccg gatcggcccg atcagcaaac tgggatccgt gccctcaccc
     3361 ggtctcgcac actgctcctg agcaggggct cagcaccttc tcctcgatgt ccgggctgtc
     3421 agacatccgg cctggcccga ccggtgggtc gtcggcgttg ccgtgcctgg tcctcgcaag
     3481 ctgggcgggc tgctcgagcg caaccgcggc gatgatcgcg atcacggccg cgatgacggc
     3541 ggcggccgcc gaggtcaggt ggagccgcgt cacgaacgcc tcgcgcgcga cgccgagcag
     3601 ggccgtaccc gtggcctcag ggagctgggc ggccactccg accgccgagg cggccaggcc
     3661 gccggccacg acgtatgcgg gctacgcccg ctgcaggtac tgaccgatga acaggaagta
     3721 gccgaccatc acgaagatcg ccaggaagtt gacgacgagg gcggcgttga aggcccggat
     3781 ccggaacagg ccgacgtcga tcatgcgtcg gcaagctgcc gctggcggcg cacccagagc
     3841 gcgccgacgc ccaggccgcc ggcgacggcc ggcaccacgg acacaccgtc ctgcgcgatc
     3901 tgcttcagcc cgtagaccac tgcgagcagg gcgacgaccg acatgcccgg cgctgaccag
     3961 gtccagcagg ccggcgtcgg ggtcgggata ctccggcagt accccgtggc ccgagtacga
     4021 gcagtgcgct catcacgggc agggccagga ggaacaccgt gcccggcgga cgtcatccac
     4081 cggatccggc ccggctcaag ctgcggtcgc cttgtcgccg gtgggcgtga tggcgaacca
     4141 gatgccgtcc gcgccgttct gtccggcgct ctggaggccg ccctggtcgt cgcggtggcg
     4201 gtacaggggc cagccggcga gggtgagctg ggtggtgccc tcggggcggg tgagcctgcc
     4261 gaccgcagcc gcgtcgacgc ccaggagctc gggctcctgt ccggccgcga ccaggaccgg
     4321 cagccacgtc tgagcacagg acccggagca gttggacgtc gatgggtcgg cgctgtcggc
     4381 atcggaccgg tacatgaggt gcccggcgcc gtccgtcgcg acgacaccga ggggaccgct
     4441 ctgcacggcg tacagcgaag gcccggcgtc gccgggtgag ccgtgtccgc cgtggtgccc
     4501 gccgggttgc tgcgttgcgc cgcccgcggc ggcggtggtc ggtgggctga cggccggggc
     4561 ggccggggcc ggggtgttcg aggcagcgca accggtcagg gcgaccattc cggccgcgag
     4621 gactgaacac gcgagggaga gcgaggagcg agacacggcg ggccatcctc ctccggcaca
     4681 acctcgccgt cgaccggggg ccacgacttg cgctcactcc tcgggaatct cgcgccgcac
     4741 ctcctccagc cagatgcggg cggacatgtc cgagggcgcc cgccagtcgc cgcgtggcga
     4801 cagtgatccg ccggctgaga ccttcgggcc gttgggcagt gccgagcgct tgaactggct
     4861 gaagctgtag aaccgttggg cgaagacctc cagccagtgc cggatctcgg cgagcgtgta
     4921 ggcgggccgc ttgtcggcgg ggaagccggc tggccagtcc ccgtggtccg ggtcccgcca
     4981 cgcgtgccag gccaggaacg cgatcttgga cggacggaac ccgtaacgca ggacgtggaa
     5041 gagggagaag tcctgcaggg agtaggggcc gaccgtcgcc tcgctgctct gcacctgctc
     5101 gtcctcgccg gtcggcacga gctcgggagt gatctcggtg tcgagcacgg actccaggac
     5161 ctcgccgacc tcggcctcga actgcttcga cgagacgacc caccggatca ggtgctggat
     5221 cagggtcttc ggcacgccgc cgttgacgtt gtagtgcgac atctggtcgc ccacgccgta
     5281 ggtcgaccag cccagtgcca gctcggagag gtccccggtg ccgaggacga tgccgccgcg
     5341 ctggttggcg atccggaaca ggtagtcggt gcgcagcccg gcctgcacgt tctcgaacgt
     5401 gacgtcatag gccggctccc cccgggagaa gggatggccg atctcggtca acatcaggcg
     5461 tgccgtcgcc gtgatgtcga gctcctcgaa ggtgatcccg agcgcgcgtg ccagccggat
     5521 ggcgttgccc ttcgtgcgtt cgcccgtggc gaacccgggc aacgtgaacc cgaggatgtc
     5581 actgcggggc cggccctgac ggtccatcgc cttcgcggcg acgatcagcg cgtgggtgga
     5641 gtccagccca ccggagacgc cgatgaccac cttgggctgc ccgatggcgc gcaggcgctg
     5701 ctcgagcccg gacacctgga tgctgtacgc ctcgtagcag tcctgttcga gccgcgactc
     5761 gtccgccggc acgaagggga accgctcgag ctcccggagc agcccgatgt cgccgcccgg
     5821 cgggtcgagg cggaactcga cgcggcggaa cgagtccgta cgggcggagt gatgcctgcg
     5881 gttgtcgtcg aaggtgccca tgcgcaggcg ctcggcccgc agcaggtcga ggtccacgtc
     5941 ggcgaccgac cgccgttcgc cctgggggaa gcggtccgac tccgccagca gcacaccgtt
     6001 ctcgtagacc atcgtctggc cgtcccaggc gaggtcggtg gtcgactcgc cctcgcctgc
     6061 ggccgcgtag acgtaggcgg cgaggcatcg tgacgaggcg gaccggcaca tcaggtgccg
     6121 gtcctcggcg cggccgaccg tgatggggct gccggacagg ttcgccagca ccgtggcccc
     6181 cgccagcgtc gcctcggcgc tcggtgggat cggcacccac atgtcctcgc agacctcgac
     6241 atgcaggacg aacccgggca cgtcggaggc ggagaacagc aggtcggggc cgaacgggac
     6301 gtcgagagcc gcatccggac cgccgatgag gatcgtcgtg ctgatgtcat cgcccggcgc
     6361 gagctgacgc cgctcgtaga actcccggta gttcgggagg tacgacttcg gcaccacgcc
     6421 gaggatgcgc ccgcggtgga tgacgacggc ggtgttgtag acgcggtgcc ggaagcgcag
     6481 cggcgcgccc acgaccagca ccggcaggac gtcggcggac ccggtgacga cgtcgagcag
     6541 cgcctgttcg acggcgtcga gcaaagagtc ctggaggagg atgtcctcga tcgagtagcc
     6601 cgacagggtc agctcgggaa agacggccac gccaacgccc tcgtcgtggc attcgcgggc
     6661 aacgcgcagc accgactcgg cgttcgcggc cgggtcggcg atcgaggtac tgagcgtgca
     6721 ggccgcgacg cggaggaagc cgtggcggta cgcggagtcg aagtccatgc gggcggagac
     6781 gaccttcctc gtgaggggga caccggagat cctgtccgca gtctgcccac atcgccgggt
     6841 ctgaggatgc tcagcgcgcc gtgcgcgtgg tatccgtccg gtttggaacg aggtgtccga
     6901 cgggtaccgg agagcacttg accttgggga gctggggagc agtatgcgga tcggtggcgt
     6961 gatcgtcgtg atctggctcg tgatcgggtt gatcgcgacc tatcagcggg gctacttcgg
     7021 acagtctgac gtgaattgtg ccgcgctcgg aacggtgctg gtcaccgtcg tcgtcgggcc
     7081 gctgaactac ctcggggtca atcctcggat cgagtgccag gtgccccagc cgtcgccatg
     7141 aggacgatga ggacatcgtg aaggggcgcg gaggccgggg aaccgtccgg cgccggtctg
     7201 ccgacgccgg acggggcggt cagccggaca gccgccgggc cagcaactgc ttgccctggt
     7261 cggcgccctt gcggctctct gtctgtgctc gccggaagag ctcggcgacc tcgttgtcgc
     7321 cgtcgctctc tgcgtcctcg atgtaggtct ccaggcggag tgtgttgtcc aggcaggctt
     7381 cggtgaacca gatgaggttg tagtccttgt ccctggtccc cgtgatgtgc ccggtctctg
     7441 cggtcgccat agggtgctcc cttctcgcgg tgaagaatgc gccctggaca taccccgtgt
     7501 ggtggcagtc acacaccggt cgcgaggtct ggcacccatc tgcgaacgtc gaatgggcgg
     7561 tgccagtgga tggcgaggtc ctgggggcac gcctccccgg agaccagcag caacctcaga
     7621 tcggggaggt cgtcgtgcat cgtcgagagc agggtgggca cgcagcaccg ctgggttctt
     7681 cgaacaggtg gttcaggcgc tcgggtccag cggaccgact ggcgcagcgt cgtcgctggt
     7741 gaggaccagg tccgcgagcc ggccgaacac gccggccggc cgcgtcccct cgtcgaatgc
     7801 cgtgctcaca ccgcgacgtc gtgaaggttg gccgcagcgg actcctcgat gatgtcgaga
     7861 ccacggtgca ggagatgtgt gggagtggtc agcggggcaa ggatcttgac gatctcgtcg
     7921 tcggggcccg cagtctccac gatcagctgc tgacgcgcgc acagccggca gatctcggcg
     7981 gcgacgctcc cgtcgcgaac gtcgatgccc tgcatcatcc cgcggccctt cacccgggcg
     8041 ccgggcacga gcttcgcgat ctgtgtcagc cgctcgccga gcagtacacc gcttgccacc
     8101 acgtcctcct ggaagctgcg gtcgccccag aacttctcga gcgccacccg cgcggtgacg
     8161 aacgcatgcg cgttgccgcg gaaggttccg ttgtgctcgc ccggtttcca gatgtccaac
     8221 tccggcttga gcaggagcag cgccatcggc agcccgaagc cggagatcga cttggacatc
     8281 gtgatcaggt ccggggtgac ccccgtgccc tcgaagctga agaagctgcc cgtgcgtccg
     8341 caaccggcct ggatgtcgtc gacgatcagc agtgcgccgt acgtcttcgc gatggcagcg
     8401 atgcggcgca gccacggccg ggaggcgacg ttgaggccgc cctcgccctg cacggtctcg
     8461 aggaggatcg cggcgggttc gtcgacgccg ctggagtggt cggacagcat gcggtccagc
     8521 aggtccgcgg tgtcgacgga gccaccgagg tagccgtcat agggcagccg ggtcacgtcg
     8581 ggcagggaca gcgtcgtccc catgcgcttg tcccggttcc cggtggcggc cagcgccccc
     8641 tggctgacgc cgtggaaccc gttggtgaac gcgatgacgt tgcggcggcc cgtgaccttc
     8701 ctggccagct tgagagccgc ctccaccgcg ttcgtcccgg tcgggccggt gaattgaacc
     8761 cggtagtcca tgccgcgcgg ttcgaggatc aggccctcga aggcctccag gaactccatc
     8821 ttcgggcggg tgaagaggtc caacccgtgc gtgatccccc cgcccctgat gtgcgcgacc
     8881 agggcatccg ccatgtccgg gtcgttgtgt ccgtagttga gcgccccggc tccggccagg
     8941 aagtccaggt actcccggcc ctcggtgtcc cagaccaggc tcccgcgggc ctcctcgaag
     9001 acgacgtcgt acttgcggct gtaactgcga acgcgcgact cacgccgcgc aaagacctca
     9061 ttgccggtcg agaccgggcg ttcactcatc atgtctgggc ttacgcatcc accctggact
     9121 ttgttcaatg cacagccgtg accatactca cggcatttgc gcaaggggct gaaccactaa
     9181 gtcgtaacgg ttgcgtaaac gcttgaacct gcatggagcc ctcgtgacag gcactaaccg
     9241 gacaccctgg aggcgttgcg ggagccctgc ggaaatcgat accaggatgt gattcaaccc
     9301 attctgaggc attcgatacg tcaagcggtc gcgtggacgc ccggggccgg tgacgccgct
     9361 gacgtgctcg tcatggaggt tggggctgtt cagacgggcc agtgcagcgc ccagcccacc
     9421 cgaccgcgca acgcgggtca ggctaacgtc ccggtcgacc cgatgatcgg aggacaacgg
     9481 tgacggacgg ctcggtagcg gtggtgacgg gcgcgggttc cggcctgggc cgggtcattg
     9541 cgcgggcgct gctctgcgcg agtttccggg tagctctggc cggtcgccgc cgtgatgctc
     9601 tggaagacac ggcagccggg gagccggggg cggtggcatt gcccaccgac gtcgccgacg
     9661 cggcgtccgt cgcgcggctc ttcggagccg tgcgcgagac gtgggggcgg gtggatctgc
     9721 tggtcaacaa cgccggcaca tccggcccga gcggcagcat cgacgagatc gccgtcgagg
     9781 actggacggc cacggtgggc accaacctca ccggggcgtt cctctgcgcg cgggaggcct
     9841 tcgccgcgat gcgcgcgcag cagccgcagg gtgggcggat catcaacaac ggttcgatct
     9901 ccgcccacac cccccgaccg ggtagcgccg cctacaccgc gacgaagcac gcgatcaccg
     9961 ggttgacgag gtcgatctcg ctggacgggc ggccgtaccg catcgcctgc gggcagatcg
    10021 acatcggcaa cgccgccacc gacatgaccg ccaggatcgc caccggcgtg cggcaggccg
    10081 acgggagcgt ccggccggag ccgaccttcg atgccgctca cgtcgctgat gccgtgcttc
    10141 tcatggcccg cctgccgctc gacaccaacg tgcagttcct cacgatcacc gctacgtcca
    10201 tgccgttcat cgggcgcgga taagggcccg tgcgcggata cgagcgcgcc agcacccgta
    10261 tccgcgcacg accgctcagt gggtgcggat catcccgccg tcgcagcgga cctgctcgcc
    10321 cgtcacgtag gacgccgcgg cgctcgcgag gaacgtgacg acagcggcga actcctccgg
    10381 cgtgccatag cgcctggcgg ggatcgccgc ctcggcccgg gcccgctgct gttccggcgt
    10441 ggtgcccgag cgctcggcga tggcgcggtc cagcacggcg acgcggtctg tctcgatgcg
    10501 gccgggcagc accatgttca cggtcacgcc gtccgcggcg acctcaccgg cgagcgtctt
    10561 gaggtagccg gccagggcgg cgcgcgctgc gttggacgcc acgagcccct cgatcggctg
    10621 ctgcacgccg ctcgatccca ccgccacgat ccggccgaaa ccgcgctcac gcatgcccgg
    10681 cagcaccgcg gcgacgagcc ggaggtgagg ctgcacgagg aggttgaccg cggctgcgaa
    10741 ctgctcgggg gtgaactcgg cggccgtccc cgggggcggc cccccaccgt tcagcaccag
    10801 cacgtccacc tgtccgaagc gctgctccgc ctccgccacg agcgtcgcgg gcgcgtcggg
    10861 atcggcaaga tccgcctcga ccccgacggc cgacggcagc ttggccgcct ccgcccgcac
    10921 cagctccccg cgccgcccgg ccagtacgac gttggcgccc tccgcggcga gcgtgcgggc
    10981 gacggcgagc ccgagcccgg agctcgaccc cggcacgatc gcggtgcgtc cctgaagtcc
    11041 cgtgtccatc aggtgctcac tgccgtcggt acgtcccgca ggcctcggct actgggttcc
    11101 atgtgcacgg agtcaaccac ctcaaagctc ccgcgccacc gggccgtaca ccgtgacgat
    11161 ctcgtcgagc atggcccgct ggccgtgcct gagtccgtcc gccgccaacg acccgagccg
    11221 cgcacctggt gcgtgctcga gtacgtgtca ggtgcgcgac gtgtacgcca catacacggg
    11281 ctgcacgggg cagtactctc agacatccga tgtcttggga ggttgggtgc cggtcactga
    11341 cgaagccatc gagaagatca aggcgatgat cgtctccggt gagctgggcc ccggcgcgcg
    11401 gctgccgcgc gaggcggatc tcgccgagcg actggggctg tcgcgcaact cgctgcggga
    11461 ggcggtcaag gcgctctctc tgatccgggt gctcgacgtc cgccagggcg acggcaccta
    11521 cgtcaccagc ctggacccgc acctgctgct cgacgcgatg accttcgtcg tcgacttcca
    11581 ccgcgacgac acggtgctgg agttcttcga ggtacggcgc atcctggagc cggccgccac
    11641 cgcgatggct gccctcacga tgaccgagga gcaggtcgac ggactgcgtg ctgtgctcgc
    11701 ggagctcgac caggatcccg gcgtcgaggc tctcgtggcc aacgacctgg agttccaccg
    11761 cagggtggcg gtcggcagcg gcaacaccgt gctgtgctcg ctgatagagg gcctctccgg
    11821 cccgacgacc cgggcccgga tctggcgcgg gctgacacag gagggcgccg tcgcgcgcac
    11881 catcgaacag cacgccgcca tctgcgacgc gatcgctgca cgtcagcccg acatcgcccg
    11941 ctcgtgggcc accatccacg tggccggcgt cgagcagtgg ctgcgcagcg cgctgggcga
    12001 ctctccctga cgcgactccg cgcgctcggt gttgtcgtcg caggaccact gggctctgcg
    12061 aggagcaggg accggcgcgc ggctacgtcc tgtggatccg gcgcgccgtg tcgcccagca
    12121 ccgctgctcg agtcagtcct ggcgcggccg caaccgcagg ccctgcatac cgccgtccac
    12181 gaagagcgcg gtcccggtgg tggcggccga caccgggctc gccaggtacg cgatggccgc
    12241 ggccacctcc ggtgccgaca ccaggcgtcc catcggctgg cgcgcctcca gcgcggcgcg
    12301 ttcggcggcg gggtcactgg ccgcgtcgag cagcctgccg acccacggtg tgtcggccgt
    12361 tccggggttg acgcagttga cgcggatgcc ctcggcgacg tgatcggccg ccattgccag
    12421 cgtgagcgac tgcacggcgc ccttgctcgc gctgtacagg gcccgctggg gtaggccggc
    12481 cgttgccgcg atcgagcagg tgttgacgat cgccgcggcc gacgaccggc gcaggtgcgg
    12541 caacgcggac cggctgagcc ggacgatgcc gagcacgttg acgtcgagga cgcggtgcca
    12601 ctcgtccagc ggattggtct cgacagtgcc ctgcgcaccg attccggcgt tgttcaccag
    12661 cacgtccagt ccgccgaagc gctccaccac ggtggcgact gcggcgtcga cggagtcctg
    12721 gtcggtgacg tcggtggcca ggccgagcag cggcgcgtcg acgccggacg ggtcgaggtc
    12781 gaggcaggcg acgcgggcgc cgcgttcggc gagcagccgc gcggtggcca gcccgatccc
    12841 ggagccgcca ccggtgacga tcgcagccag cccggcgaac tcgcccccgc ctcccgcggt
    12901 cactgcgcgc tccgctgcgg ttcgcgggcc caggccgcgc cgtcggggac gcggtgtgtg
    12961 gccagggtca ggggacacat ctcagcgccg gagcccggcg cggtcggcgc caggtactgt
    13021 ggcgccacac gtagaagccg caccgctact ccctcagaca tcggatgtat cgtcggtctt
    13081 tatacggcca atctccgagt ccgtgcaagc tcggggggag gttctcgcat gagacatccg
    13141 atctctgccg gctcgtcggg ttcgatggta ttgccggaat caggccgcga cgctttccct
    13201 cgggctctgc cgaccgcggc ggggcggcgc cccgtgaggc gccggccgtg cccgcattga
    13261 tcgggaagtg gatgcctggc cgaggcgtca cgttctgagg atcaccgcgt gcaggtccgc
    13321 cgcccgcggg gccggaacgg agcgggcggc ggccagccag gcggcggcgc gtgcggtgtc
    13381 gcccgcggtg gtgacgcaac ccggccagcg ggcggagagc cgcgtgcgca ggagttcggc
    13441 aaccggtgtg tcaccgcccg cgacactccc ggccaggacg atcggcgtgc gctcgtcggg
    13501 gtggcggatc gcgtgtacgt cggccaccag cgcggttgcc gccgccgcca cgatggtgcc
    13561 ggcgacggcg tccccggccc gggcggcgct gctcacgagc ggggcgagtc gcgcgagcgc
    13621 gatcggcggc gcggcgtgga ccgtgtgcac gatctgttcg gccgtgacgc cgctgtcggg
    13681 agtgttcacc gcagcaccga gcaacgcggc ggtcacggcg gggagaaggg ccgtcggtgt
    13741 gcgccggccg tccagtgcag cgagggcggc gcgcacagcg cggcggccca gccagaacgc
    13801 cgacccgacg tcgccgacca gccagccgtg accgtccgtc ccgtcgacgg gttcgaggcc
    13861 ggcgatccgg gctgccgtgg caccggtgcc cgagatgagg acggtgccct cccgctcggg
    13921 agtgcctgcc gcgaaggcga cgacaacgtc gcccacgacc gtgggccggc agcgcaggcc
    13981 gacctcgacg gccaggtcgg cgaggctcgc cgacaccccg ggcaaccgga cggacccgac
    14041 gcccgccatc ccgatcacgg ccgcccgcac ggcggacgcg ggtaggccct gcaggacggc
    14101 ccgcatcgcg cccgccagcg ctcggaatgc ctggtcggga gggtgcgaga cggggttccc
    14161 gcccggcccc tccgctgccc cgcacacccg cccggcgccg tcgacaaccg cggcccgaga
    14221 gcggctgccg cccacgtcca tcccgacgaa gaggtccacc cggcgctccc ttaacagaaa
    14281 cgtcttaccg aagtcttgac agttcacctg actcggtgga aattttcacc accttgctcc
    14341 ggctcgaaag gttgtactgg tggctcaagt tgctggatcg gacactcctg ccgaggcggg
    14401 caccggtcag ctgctcgtgc acctccgcag cctgctcccc gggctcgccg gagcgttgcg
    14461 gcaggtcgcg aacgtgatcc tggaggatcc cgcgtgggcg tcccgggcga ccatcgtgga
    14521 gctgggggag cgcagcggta cctcgcccgc cactgtcacc cgcttctgcc gcacgctggg
    14581 cgtcgcgggt tatacggagc tgcgcgtggg catcgccacc gagatcggac gcgccgacca
    14641 ggccggatgg gaggtcggca tcggcgccga cgtactgccg accgacccgc tcgaccgggt
    14701 gttgcggacg ttgctggccg tggacatgca ggcgatgcac gagaccgcgg cggccttgga
    14761 cctcgcagtc atcgacgcgg tcgccgacgc ggtggtggcc gcgcggaggg tcgacttcta
    14821 cgccaccggt ggcagcgcag cggtgctcga ggaccagcag ctgcggctgt accggatcgg
    14881 catcgccacc tggacctggg ccgacgtgca caacggcctg accagcgcgg ccctgctcgg
    14941 agccggtgac gtggccatcg cgctgtcgca cagcggccgc accaccgaga ccgtcgagat
    15001 gctggccgag gcagggcgcc gcggcgccac gacggtggcg atcaccaact tctcgacgtc
    15061 gccgatcgcc gacgtcgccg accacctgct gggcacctcg gtacgggaga cggcgttccg
    15121 cgccggggcg atgtcggcgc ggcactccca gttcctggtg ctcgacctgc tctacctggc
    15181 tgtcgcacaa cgcacgcacg agcgctcgac ggaagccttc gcgcgcaccg ccgacgccgt
    15241 ggccgggcac cgcaccccct acgagccgcg cgcgaggggc ggatccgcgc gctccgtcga
    15301 cccgcaccga ggagcctgat gacgtccgag aggcacacca cgagcgaagc cgcggcgtcc
    15361 gaccccgtct cggcagcagc cttcgccgag gcagcagctg tcgctgtccg gcgggtgtcg
    15421 accgagcagg cggacgccgt cgcggcggcg gcggccctgc tggtcgacgg attgcgggca
    15481 ggtggggtgg tgcaaacctt tggtaccggc cactcccagg cgctggcgat ggagatcgcc
    15541 ggccgcgcag ggggtctcgt gcccaccaac atgatcgccc tgcgggacct ggtgatcctg
    15601 ggcggtgacc ggccggacgt gctgttcaac gagcacctgg agcgggaccc ggcggtggcc
    15661 caccggatct acgagctggc cggcgtgcac ccgtccgacg tgttcgtgat ggcgtcgaac
    15721 tcgggcggca acggctcggt cgtggagctg gcgcggatcg tcaaggagcg cgggcacggc
    15781 ctggtcgcca tcacgtcgat ggcccacagt tcgcgggtga cctcgcgtca cccctccgga
    15841 ctgcggctgt tcgagctcgc cgacgtcgtc ctcgacaacg gggcgccgta cggggacgcg
    15901 gtgctgacca cgggcgagct cgccgtcggt gggatctcgt cgatcacggc cgccctgctc
    15961 gcccagatgg tcgtcgccga ggtcgtgcgc acgatgctcg ccgcaggcga ggctccaccg
    16021 gtctacctct cggccaacat ccccgagggc gacgcccaca actccgcact ggaagcccgg
    16081 tacgccggcc gcatccgcag gtccgcctga gcaccgccct cccctcctcg tccgctcccc
    16141 gccgacacca cctgccctcc tcgccccacc ctcgttgatc aactgcacca ccaccggttc
    16201 ccccttcagc tcaaggagac agccatgaac ccgtctcgtc ccgtgctcgg actcgaccgc
    16261 cggcagttcc tgcagcggct ggcggccgcg ggcctgctcg cggtgccagg agcagggttg
    16321 ctctccgcgt gcgccaccgg gggcggggga accaccaccg ccgcgcaggg ccaggtcagc
    16381 gccaccaacc cgctcggtgt tgccaccgac gcgccgctgg acgtcgtgat cttcaagggc
    16441 ggctacgggg atgactacgc cctcgcccac gaggcgatgt acaaggagca ctacccgcag
    16501 gcgcaggtca agcactcggg gagcacccag atcgcgcagg agatgcagcc gcgcttcgtc
    16561 gcgggcaacc cgcccgacgt cctggacgac acgggctccc agaagatcga gtacgcggtg
    16621 ctcgtgaacg acaaccagct gaccgacctg ggcccgctgc tggacgcccc atcggcggat
    16681 gacccgaacg tcaaggtgcg cgacacgctg ctgcccggcg tgatcgagca gggcaccttc
    16741 ggcgacaagt tcgtcatcct caactacgtc tacgagacct acgcgctctg gtactcgaag
    16801 ccgctgttcg agaagaacgg ctggcaagcg cccaccacgt gggacgagat gatggcgctc
    16861 tgcgagaaga tcaaggccgc cgggatcgcc ccgttcacct acggcggtgt caacgcgagc
    16921 gactacgtca tcgacctcaa cctgggcgcg gccatcaagc agggcggcct cgacgtcgtg
    16981 aagaagatgg acaacctgca gccgggagcc tggacgcagg acacggtgct ggcggcgacc
    17041 cagcggctcg aggagatggt ccgcaagggc tacttcatgc cgggctcgga gggcctcaag
    17101 cacaccgagg cgcagacggc ctgggtgcag ggcaaggccg cgttctacgt ctccggctcg
    17161 tggatcgaga acgagatgaa gggcatcacg ccgccggact tcgcgatgac cgcggcgccc
    17221 attcccgtgc tgggcagcgg ctcggccatg ccggtcgctg cgttgcgcac gcagccggcc
    17281 gaggcgttcg tggtgccggc caaggccaag aacgtgcacg gtggcatgga gtacctgcgg
    17341 ctgatgctgt ccaagcaggg cggtgagaag ttcgccgagc tcaccgcgtc agtgcccgct
    17401 gtgaagggcg ccaccgccaa cgcgaagaag acgccggcgc tggagtcgct gtcggccgcg
    17461 ctcgccgccg ctgggagcaa cgtcttcagc tggcagttcc ggacctggta ctcgagctac
    17521 agcaccctgc tgatcaccga gacgaccaac ctgctcaccg ggcgcacgga cgcggcgggc
    17581 tacctcgcga agctgcaggc ggagaccgac cggatcgccg cggaccagag catcgccaag
    17641 taccaccgct gaccagccat gcgccacgga aaataccggt tcatcgccag cttcctggcg
    17701 atcccgctgg tgctctacgt cgtgttcgtc gtgtcgccgt acgcccaggc gttctacatc
    17761 tcgatgaccg actggcgggg cttctccggt cagcagaact tcatcggcct gcgcaactac
    17821 gccgagctgg ccaccgacgg ccggttctgg caggcgatgg ggcacaacgg gctgctgatc
    17881 ctggtggtgc cgctggtgac gatcgcgttc gccctgttcc tcgcggccgt cacgaccgtt
    17941 gcgggccgcc agggtgcggt gggccgcggc ggtgtgcggg gatcacgctt ctaccaggtg
    18001 gtcttcttct tcccccaggt cgtcccggtg gtgatcgccg gggtgctctt cgggttcttc
    18061 ttcaaccccg acgcgggcct gctcaacggg gtgctccggg tggtcggtct cggctggctc
    18121 acccggccgt ggctcggcga ccctgcgctc gcgctgccgt cggtgatggc cgtgctcatc
    18181 tggggcgcgg tcggcttcta ctacgtgctg ttcagctcgg ccatgaagtc ggtgccgccg
    18241 gacgtcttcg aggcggcggt gctcgatggc gccggccgtt tgcgcacctt ctggagcatc
    18301 acgctgccga tgatctggga cagcgtccag gtctcctaca tctatctggg gatcatggtg
    18361 ctcgacgggt tcgcgctgct tcaggtgatg ctgccggtgc cgggtggcgg cccggacggg
    18421 tccacggtgg tggtgtcgca gtacctctac gagaccgcct tcaccggggg ccggttcggc
    18481 tacgcgtcgg cgatcggggt cgcgctgtgc ctggtgacgc tgttgctggc ggttgccgtc
    18541 ctgcggctga cccgccgtca acagggcgag tactgaggag gacggcaatg actctcgaga
    18601 catcggcccc cgcgacggcc cctcccgccc ggccgcagcg gcgggccagc cggcaggaca
    18661 ccgccggaag gtcgaccggc gagcggacgc tcggcgtctt cacccacggc ttcctcgtcg
    18721 cgtgggcgat cctggtggcg gtcccgctgc tctgggcggt gctgaccgcg ttcaagaccg
    18781 acagcgagat cttcaccaac ccgtggagcc tgcccgcgca ctggcagttc ggcaacttcg
    18841 cgcgggcctg gaccacggcc aacatcggca cgtacttcgt caacagcctc atcgtcgtca
    18901 gcgtgtccgt cgtgctggtg atgctgctgg gctcgatggt ggcctacgtc ctggctcgct
    18961 acgagttccc tgggcggagc gtcatctact acgcgttcgt cgccgggatg acgttcccga
    19021 tcttcctggc cctcgtgccg ctgttcttcg tggtgcagaa cctcgggatg ctcggcacct
    19081 accagggact gatcctcgtc tacacggcgt acgcgctgcc gttcaccgtc ttcttcctca
    19141 ccggcttctt ccggacgctg ccgggggcgc tcgccgaggc ggcgatgctg gacggctgct
    19201 cgcacgccgg ggtgttcttc cggatcatgc tgccgctcgc acgtccgggg ctgatcagcg
    19261 tggggatctt caacttcctc gggctgtgga accagtacct gctgccgctg gtgctgatgc
    19321 ccgacagcca gcactacgtg ctctcccagg ggctcgcggt gctggcggcg aaccagggct
    19381 accgctccga ctggagcgcg ctgttcgccg gcctggtgat cgccctgctg ccggtgctcg
    19441 ccgtgtacgt catcttccaa cgccggatcc aggaggggct gagcgtggga gccatcaagt
    19501 aggggcctga acaccgtggt cgtcaacacg gtgaggcgcg gatcggtgcg gcgtaccgag
    19561 tgatcggctc cagggcgcga ctccgggcac tgcgctgggg tctacggatc atgttcggac
    19621 tcccgcagcc cgtcaagcgg gtgctggcgg gacgccgcgt cgagcgcgac gggcaggtac
    19681 tcgacctgga cctgcagctc ttcgggcgga tcgacgtgtt gttggccagc tccgccggtg
    19741 ggacggtcga ccaggccgcg ctggccgagc agcgccggca ggccgacctc gcggccgagg
    19801 tcgtggccgc cgccggcctg gacgacgtgg tcaccgagga cctgtaggtg ccccgtgggg
    19861 ccggaccgct cgccgcccgg ctcgctgtgc ctcccgccgt gccggagacg gccggactgg
    19921 tctacttcca cggcggcggg ttcgtcatgg gcagcatcgc ctccaccgat ccgctgtgcc
    19981 gcctgctcga ggcccaatcc ggtgtgcgcg tgctctcggt cgactaccgg ctcgccccgg
    20041 aggaccccta ccccgcggcc ctcgaggacg ccatcgccgc ctaccccggg ccagggccta
    20101 cgcgacgggg tgccgtccga cgacatgcca tcctgcgggc cgacgacctg tcggggatgc
    20161 cgcccgcgct ggtggagagc aagctgacgg gcaggtcgac ggtcgatcgg ccctcgagct
    20221 catccgcatc ccccgggcat accgagcggg gtaggcgtgg tcgctgcgcg acccggaacc
    20281 ggcgcgtgtg cgtccaggtc gagccccgac cgggcgctac cgtcaccggg acgagggtgg
    20341 atcagtctcg ttcactcgac ggtcggcccg atcggcgaga gtaggaggcg ctgccggcgg
    20401 atgtcgcagc gcggctggag gcgcgcccgt gaatcgccag gacaccacag tcaccctgcc
    20461 ctggatgggt gccggcaccg agttcctcgg ccgaatcgtc gacgtgctgc ccgacgacgc
    20521 gttccgggcg ccgagcgcgc tgcccgggtg gagcagggcc cacgtcgtcg cgcacctcgc
    20581 gcgtaacgcg gaggccctca gccggctcgc gaactgggcg cgcaccggca tcgagacgcc
    20641 gatgtacgcc gaccgcgagc aacgggcagc agagatcgag tcgtcggcgc aggcaccggt
    20701 cgagaggctg cgccgggagc tggtgagcac cgccgatgag ctcgacgcgg ctctcgccgc
    20761 gctggacgac gcggcgtggc gggccgaggt gcgcaccgca ctcggccggg cgatacctgc
    20821 caccgaggtg ccgtggatgc gggtgcggga ggtgtggctg cacgccgtcg acctcgacgc
    20881 cgggggcgcg ctcacggatc tcccgctgga gattgtcgac gcgctgctgg acgaggtcac
    20941 ggtcacgctg tcgtcgcgcg agggctgccc cgccgctcga ctcgctccgc tggaccgcga
    21001 tcgaacgtgg ccgctcggcc cggagccggc cgagcggatc gtcgaggtgc gtggtgacgc
    21061 ggcccgcgta ctcggctggt tgatcggccg gagcgccggc ggtgtcgatg cggtcgacgc
    21121 aggcggcgcg ccggctgcgg tcccggcggc gccgcgctgg ttgtagggat tgcggccccg
    21181 gtcccgaccg cgcgtagggc gcgcccgctg cgcccgggag cgcggcggtc gtagcccagt
    21241 cagactggcc agaagtagcc cgcctcggga tccgcgtcgg agaacagcgg gccgtagaac
    21301 tccgggtcct tgcgcaccag tgcggaccgg tggctgcggt ggacacgctc gtcgccgatc
    21361 catttcggca gcaggctcag cgcggcaagc tcggcctgcg tgcgcggcgg tggcagcccg
    21421 tgctcggcga ggtcggtgcc gatcgtggcg gcgcacgtgt cggcacgacc ccgccccacc
    21481 cactcggcgc agatcaccag accgtacgcc gcgacgccct ccggataccc ggcccacatc
    21541 cgcacggccg ggtgccgctt ccagccgtag gtcgcccggg tcacggcgcg cagcacctgc
    21601 atggcctcca cccgctgctt gcccagccgc aggttgtcga gcaccgcggc gctcgccgcg
    21661 aagtcggggt agggcaggaa cgtctgcatc tcgagcggcc tacgcctgct ggacgagggt
    21721 cacgacgcgg gtcagcgacg gggcgcgggg cgtcgatccg aatccgaacc ggacgggggg
    21781 cgctaccggg gcgaggacgc cctggtccgc gccgtcccaa gtcgtggacg ccgccgtcag
    21841 gccgtgcagg tcgagggcgg cgtagtactc ccggcgcccg ccgacggcac tgcccgtggt
    21901 gcgcaccccg ggcagcaggc ggcgcgctac gacgtcgatc gcgttgatcc atcgtgggct
    21961 cgtcgccatg ggcctgggca ctgcgcagag caggagtccg agaggggtcc gcgccccgac
    22021 cgtgaacgcg aggtccagcg gcgcagcgtc gacccgccac cgtgctccat cgacctccgc
    22081 gtggaccggg gccacccgca cctcgtcgaa cacgtacgtg gcagccacga aatcggcgac
    22141 ctcgcccgac ggtgcgagca gcacgcgatg cccgtccggc tgctccacca tgacgtcggt
    22201 gaacgctccg agcggcgagc gcggccagca cccgaccacg acgcgcagac cggccgccgt
    22261 ccccagcccg gcgatccagc cgtcgaagcg gtcggtcatc gtgccccctc cggttccccc
    22321 ttactacccg cctcgacgct gtcctactgc tcggccgcgc ctgggagcca cgccggtcgc
    22381 cttcgctccg ccgaccgcac gccgcacgac ggggagacgt ggcggtgccc cgcctgccag
    22441 gctgggcgga gcggggccgg aatgttgctg atgcaagtac cgttgagaca gccacagaac
    22501 gtccaggaga tggaggatcc gatgccacag ccagagggcg ccgagctgga agccgtccga
    22561 gcgagacggg tcgcgctcaa ggacaagtac ctgcgtgcgg tgggggagcg tccggcgacg
    22621 accgtgcgcg gtgtgcacca cctcgcgctg atcgcgcgcg acgtcgagga gacgatcaag
    22681 ttctaccagg acctgctcgg gttcccgctg gtcgagctcg tcgagaatcg ggactaccag
    22741 gggtcgagcc acttcttctt cgacatcggc aaccgcaacc tcctcggctt cttcgacttc
    22801 cccggccacg accatcccga cttctccgag acgatcggag gggtccagca tctcgcgctg
    22861 tccacgtcgc ccgaggagtt cgccgctgcc cgcaagcgcc tcgacgacgc cggcgtcgac
    22921 tatctcggtc cggaccgcgg cgtcgacaac agcctctaca tccgtgaccc gaacggcgtc
    22981 gggatcgagt tctactgcga ggagctcggc aagttcgaag gggagccgct gctctcctca
    23041 taacgtccac tgagcgagga gtagcgacgg tcacgcaacc ggtgcggggg catgcgcgtc
    23101 ctctcttgtg ggacaactgc gccgcgtgat caccgctacc gttcgccgga cggggccgcg
    23161 cggccgggat ggtgggcagc atgaacggtg agtaccgagg acggggcgcc ccgcccccgc
    23221 cggcaggccg ctcggggcgg ccgccgcgcc gcggtggtgg ccccggctgg gg
//
LOCUS       DASRSC010000246         3182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4188991,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000246 DASRSC010000000
VERSION     DASRSC010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4188991"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..484)
                     /locus_tag="VFE39_12005"
     CDS             complement(50..484)
                     /locus_tag="VFE39_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6259366.1"
                     /translation="MKIADILAAKGRTVHSVLPWLTVGEVAERLGRLGVGAVLVCDEN
                     RAIRGIVSERDIVRALGRDGAALLTKQVSEVMTRHVSTCEPEETVAHAMARMTAGRYR
                     HLPVVVDGALVGMVSIGDLVKNRLREMELETGVLRDVVIARY"
     gene            1045..1290
                     /locus_tag="VFE39_12010"
                     /pseudo
     CDS             1045..1290
                     /locus_tag="VFE39_12010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019807301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
     gene            1352..2923
                     /locus_tag="VFE39_12015"
     CDS             1352..2923
                     /locus_tag="VFE39_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HET6259367.1"
                     /translation="MSERSRSEEAPIRVGNRYRLDERIGAGAMGAVWRGTDELLNRTV
                     AVKELLAAAVPATAAGGDALEESRQRILREGRIGARLQHPHVISMFDVVVHEDRPWLV
                     MEYLPSKSLAQVLGEKGALEPREVADVGRQVADGLAAAHVAGVVHRDVKPGNVLLAED
                     GRVKLTDFGVSRAVDDVQLTRTGLIAGTPAFLSPEVARGGEPTAASDVFALGATLYAA
                     VEGTPPFGLNDNAYALLHKVATGEVRPPKQAGPLTGVMMRLLSTDPTDRPSATQARDA
                     LAAVANGRDSGVVAPAAAAAPGATAIMGAATTASPAPPGPPTTVSAAPPSGQEPDPPR
                     RWRRSALLAALAVLLLGGGLTAAVLLWGDGGNPAGSHAAPSAVNTPSAAAPSETPDTS
                     APAIPAPQPSPTHSRTPAPTTSPEPAPQSAPSDPVGFIQNYYSLLPGNTAAAWDLLSP
                     SAQDASGGRSGFESFYGRMQSVSLENVRQTGDDTVEATVVFAEQNGRTTREPYRFVMG
                     TDGDERTIIESFSKA"
     gene            complement(2924..>3182)
                     /locus_tag="VFE39_12020"
     CDS             complement(2924..>3182)
                     /locus_tag="VFE39_12020"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HET6259368.1"
                     /translation="LSPIHRVDRITAPLLVVHGLHDTNVPIAEAQQMVDALRERGASP
                     GFLLFDDEGHEVRGTENKILFVREVVRWVTAHLQEVGEQTA"
BASE COUNT          428 a         1162 c         1116 g          476 t
ORIGIN      
        1 cgctcccgct cgcctgctcc ggcgcgggag cgccgttggt cgtgagcggt cagtagcgcg
       61 cgatcacgac gtcgcgcagc acaccggtct ccagctccat ctccctgaga cggttcttca
      121 ccaagtcacc gatgctgacc atgccgacca gcgcgccgtc gaccaccacg ggcaggtgcc
      181 ggtagcgtcc ggcggtcatc ctggccatcg cgtgggccac cgtctcctcg ggctcgcacg
      241 tgctcacgtg ccgggtcatg acctcgctga cctgcttggt gaggagtgcg gcgccgtccc
      301 ggccgagcgc ccggacgatg tcgcgctcgg acacgatgcc gcggatcgcc ctgttctcgt
      361 cgcacacgag gacagcaccg acgccgaggc ggccgagtcg ctcggcgacc tcacccacgg
      421 tgagccacgg caacacggaa tgtacggttc gtccctttgc ggcgaggatg tccgcgatct
      481 tcatgtactc tccgccttcg tcgtcgcttg tggttcaacc gctgtgtctg atgatccccc
      541 cagaccccct ccaacgggtg gcatcgtcgc cccgatcacc cgctcggcgt acggctgctg
      601 tgctatgtcg tgatccgtgc gcccaccggc ttgccaggtg cctcgcgggc ggacgccggc
      661 ccgatcggta acggtcgcga atagcgccgc tcggccacga ggacggccgt cagcgctggc
      721 cacgccgtcc tgacctgcgg gggaagcaga tcgcctgcca ccgaacggca ggtcgtgccc
      781 gacacgggct ccgtcgttcg gcctcgcgcg gcccacctgc ctgcctagcg tccgcccgtg
      841 cgtcgtccgt cattctctga tgccgttctg ccgccggtgt tcgcggtgct cgccgtggct
      901 gcggtgctgg gcggtgttgt ctcgctagcc gtgtggatgg gcgttgccgt ggaggcgcgt
      961 gcgacggtgc ctggcagcga tcggtacgtc accggggcgt ccacgcgggg gaagagcggc
     1021 acggcgggcc ccactacgga gcctgtctgg gatcggctcg cccggtgcga gtcgtcgggc
     1081 aactgggcga tcgacaccgg caacgggtac ttcggcggac tgcagttcga tgctgtcacc
     1141 tggcgcgcct acggcggcat cgagttcgct cctgcggcgc accgggccac ccgggagcag
     1201 cagatcgccg tcgccgccag ggtgcgcgac gaccgggacg gctatggttc ctggcccggg
     1261 tgcgcgatca agctcggcct accgagatga cagaatgcga cagggaccgc gttcggcggt
     1321 gccgttgtgg cgatcgctac cctgacgggc gatgagcgag cgctcgcgat ccgaggaggc
     1381 ccccatccgc gtcggcaacc ggtaccgcct ggacgagcgg atcggggctg gcgccatggg
     1441 tgctgtgtgg cgcggcaccg acgagctgct caaccgcacc gttgccgtca aggagctgct
     1501 cgctgcggcc gtcccagcca cggcagcggg cggcgacgcg ctggaggagt cgcggcagcg
     1561 catcctgcgg gagggtcgga tcggagcgcg gctgcagcac ccgcacgtga tcagcatgtt
     1621 cgacgtcgtg gtgcacgagg accggccgtg gctcgtcatg gagtacctcc cgtcgaagtc
     1681 gctggcccag gtgctcggtg agaagggcgc gctcgaaccg cgggaggtcg ccgacgtggg
     1741 ccgccaggtg gccgacgggc tggccgccgc ccatgtcgcc ggggtggtgc accgtgacgt
     1801 gaagccgggc aacgtgctcc tggccgagga cgggcgcgtc aagctcaccg acttcggcgt
     1861 ctcccgcgcg gtcgacgacg tgcagctcac ccgcaccggg ctcatcgccg gcaccccggc
     1921 cttcctctcg ccggaggtcg cccgtggtgg cgagccgacg gcggcgtccg acgtgttcgc
     1981 gctcggggcc acgctgtacg cggcggtgga gggcactccg cccttcggac tcaacgacaa
     2041 cgcgtacgcg ctactgcaca aggtcgccac cggcgaggtc cggcccccga agcaggccgg
     2101 cccgctcacc ggcgtgatga tgcggctgtt gagcaccgat cccaccgacc gcccctccgc
     2161 cacgcaggcc cgcgacgcgc tggcggcggt cgcgaacggc cgcgacagcg gagttgtcgc
     2221 gccggccgcg gccgccgctc ccggcgccac cgccatcatg ggtgctgcca ccaccgcctc
     2281 cccggcccca ccgggccctc ccaccaccgt ctccgcggcg ccgccgagcg ggcaggaacc
     2341 ggaccccccg cgccgctggc gcaggtccgc actgctcgcc gcgctggcgg tgctcctgct
     2401 gggcggcggg ctcacggcag cggtactgct gtggggggac gggggcaacc ccgcggggtc
     2461 gcacgccgct ccctccgccg tcaacactcc ctcggccgca gcgccctcag agaccccgga
     2521 cacgagcgcg cccgcgatcc ccgcgcccca gcccagcccg acccacagcc gcacaccggc
     2581 gccgaccacc tcgcccgagc cggcgccaca gtcggcgccc agcgacccgg tcgggtttat
     2641 ccagaactac tactcgctgc tcccaggcaa cacggctgct gcgtgggatc tgctcagccc
     2701 gtcggcacag gatgcttccg gcggtcgctc cgggttcgag agtttctacg ggcggatgca
     2761 gtcggtgagc ctggagaacg tccgtcagac cggcgacgac accgtggagg ccacggtggt
     2821 gttcgccgag cagaacggca ggaccactcg cgagccctac cgcttcgtga tgggcaccga
     2881 cggggacgaa cgcacgatca tcgagtcgtt cagcaaggcg tagtcaggct gtctgctccc
     2941 cgacctcctg caggtgtgcg gtcacccatc gcaccacctc gcggacgaac aggatcttgt
     3001 tctcggtgcc gcgcacctcg tggccctcgt cgtcgaagag caggaagccg ggtgaggccc
     3061 cgcgctcgcg cagggcgtcg accatctgtt gtgcctcggc gatgggtacg ttcgtgtcgt
     3121 gcaggccgtg caccacgagc agtggtgcgg tgatgcggtc gacgcggtgg atcggggaga
     3181 gc
//
LOCUS       DASRSC010000247        19410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1569975,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000247 DASRSC010000000
VERSION     DASRSC010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 19410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19410
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1569975"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VFE39_12025"
     CDS             complement(<1..624)
                     /locus_tag="VFE39_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diiron oxygenase"
                     /protein_id="HET6259369.1"
                     /translation="MNVKTLSDREATAERLLRSSLDHSYEPSIDVDWEAPLVEGAWGI
                     PAHRVSLYGTELWEGLSEEQRRTLSVHELCSIARMGIWFEMILMQMLLRYAYDRDPRR
                     PHIQYALVEIADECRHSVMFAKMAERYGVPDYAPRRMTHELGRLFKTIATGPAMFAGT
                     LFVEEILDQLQREAMRDETVQPLTRMVNKIHVTEEARHVRYAREELMR"
     gene            complement(621..1520)
                     /locus_tag="VFE39_12030"
     CDS             complement(621..1520)
                     /locus_tag="VFE39_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6259370.1"
                     /translation="MTRTVTSAGTATGTMAGTRVLVTGAAGGFGAPTVARLLERGVQV
                     VGLDVAAAEHTIACDITDDAAVSRAVAEAVRRLGGLDAVVHYAGIGIPNDAGGALAPD
                     ALRVIDVNLLGAWRVTAAALPALMDARARRLGRKGRVVFVSSELAYLTLPFVSAYSVA
                     KRGMTAYADALRLEYGSELHVTTVYPGYVRTPIHDAGRELGLALEGQVRREEVDDVVA
                     TVLRALTTATPPRDTACTRRGAIELWAGRHLPGLVGSAVARRIRHRAAAGHFDGSPIA
                     AGLVRRLTGRGTDPAPIKERSAS"
     gene            1574..2275
                     /locus_tag="VFE39_12035"
     CDS             1574..2275
                     /locus_tag="VFE39_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6259371.1"
                     /translation="MPRDAPPVKAGPPSARQLSRAERRDERRDEMVQAAIDAVRTHGP
                     GVSVAEIAARAGITKPVLYRHFIDRADLQRAVGREAVALLMGRMAPELDPKREPAELI
                     RGVVDAFLAGIEDEPELWRFVVHHPIERAPGAEVVDDAREQIARLLATLIGERLRSLG
                     LDSGGAEAWAQGLVGMVESAGDWWLERRTMSRGALTDYLTTLIWGGVSGVLGGTPAAP
                     ASLRLIERDAGGHHG"
     gene            2268..2591
                     /locus_tag="VFE39_12040"
     CDS             2268..2591
                     /locus_tag="VFE39_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4873 domain-containing protein"
                     /protein_id="HET6259372.1"
                     /translation="MDDDHTEGYRGPATLTVDGRDLPIQVVLDARHEPLDGRLHWFGR
                     VVLDDGAGPELYTALTASTSRIGLSTGAATAEARIGDVDPWGRYRVTGVGVPPFPIEP
                     PIEDD"
     gene            complement(2678..2905)
                     /locus_tag="VFE39_12045"
     CDS             complement(2678..2905)
                     /locus_tag="VFE39_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 domain-containing protein"
                     /protein_id="HET6259373.1"
                     /translation="MEVKIGIAESARELVVSSDQTPDEVQALVDDALKTDDGLLKLVD
                     DKGRRYLVRASQIAYVEIAPATAGRVGFAIG"
     gene            complement(3001..3669)
                     /locus_tag="VFE39_12050"
     CDS             complement(3001..3669)
                     /locus_tag="VFE39_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like fold-containing protein"
                     /protein_id="HET6259374.1"
                     /translation="MVTARSADPAGAGRATVDLLGVLAYGELSAFDRLAEDARTAPSL
                     SGRAALSAMAAAEIGHFRMLEEHLRGLDVSVEDAMRPFVAQWDAYHASTAPRTWLESL
                     VKAYLGDGLAADFYREIGGWLPGPTGELVQQVLADTGHSAFAEREVRAACASSRQVHD
                     RLALWGRRLLGEAVTQAQQIVAERDELAEFIVSGSGDLSGIAALLKRLQSNHGKRMAR
                     LGLT"
     gene            4017..5798
                     /locus_tag="VFE39_12055"
     CDS             4017..5798
                     /locus_tag="VFE39_12055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6259375.1"
                     /translation="MDSPDNSTDAAVDEIVEAHPLQAGAPVAPDSPTFAELGVRPEIV
                     RALAEAGIVRTFAIQELTLPLALAGDDIIGQARTGMGKTLGFGVPLLQRVTPPSEQPA
                     ANADGESADRTKDVPQALVVVPTRELCVQVAKDIADAGKHLGVRVTAIYGGRAYEPQL
                     AALRKGVDVVVGTPGRLLDLAEQRHLVLGRVKALVLDEADEMLDLGFLPDVERIMRML
                     PEKRHTMLFSATMPGPIIALSRAFLNRPTHIRAEEADQGSIHERTRQLVYRAHAMDKV
                     ELLTRVLQADERGLTMIFARTKRTVQRVADDLAERGFAAAAVHGDLGQGAREQALRAF
                     RSGKVDVLVATDVAARGIDVTDVTHVVNYQCPEDAKTYVHRIGRTGRAGKEGVAVTLV
                     DWDEMPRWKMISDDLGLDMPEPVETYSTSEHLYSDLAIPTGSTGRLPRSQRTRAGLDA
                     EYVDTEGDHGGRKRGDKSSRAGRVGGARSTGAARNGDEDAERSRPRRTRARTRSRSGV
                     TVGGGDATGAETATDSAPTASTSGTVDAATTTDRPDSGPDTGAGSRAEGDRPRRRRRR
                     GGTRRRGAEGNDGEGGEQQSAPDAAAS"
     gene            5857..7131
                     /locus_tag="VFE39_12060"
     CDS             5857..7131
                     /locus_tag="VFE39_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259376.1"
                     /translation="MRLPPERRRRSDIVTAVVLALLLIGAGLTLWRTSPVAHTADDPA
                     AIPIAPPPAATDVPAGFAEAWRAPSGATDQPVVAGPAAVTADGSRVVGRDAATGAERW
                     SYARDRPLCTVDSAFPDGGVRRILALYAGTTGYCSELTALRSDTGTRLAASNPDVRPG
                     TRLLADRTYVVATGADYLEVWRSDLVKTLEYGAVTTPLQADRQPRAGCTYGSVAASGG
                     RVGVIERCPGEATDRLTVLAADGEDGADTPQVEFSVPLPSAGATLVALSADRAAVALP
                     GPPRLELLDGAGQQVGLVPLDVPDADLATDAPGRIATTESTVGSANGTGAGDGRVYWW
                     TGSRTIALDGTDLAPLWTVDGTLGPAFPYGGGLLVPVPAGLLDVDAARGTALRTLPVA
                     RADPTAPVRLAAVGNVLLEQRGTEVVALRPAG"
     gene            complement(7184..7468)
                     /locus_tag="VFE39_12065"
     CDS             complement(7184..7468)
                     /locus_tag="VFE39_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259377.1"
                     /translation="MSARRTGGWLRGLSGLLAGGLVALLVGLCIAWYIADRSGTSGPG
                     ASTLAWHAVAAVAAVLAQRHADRHRGAAGAMTACIVIAITVALLTAEWLA"
     gene            complement(7465..8376)
                     /locus_tag="VFE39_12070"
     CDS             complement(7465..8376)
                     /locus_tag="VFE39_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HET6259378.1"
                     /translation="MPVNGGAQLGFDFETTRLVRVTPSRLATWTDCPRRYRMTYLDRP
                     SPPRGGAWAHNTLGAVVHLALRSLFDLPAADRTPRAAAALVNRHWTSEGFRDAAQAAE
                     YRERAGEWVADYAAEWSAGGASIDPEWSAGRASMDTVAESDVGPVGLEQWVSVATDRI
                     LAEGRVDRIDRRGTELVVVDYKTGRRTPTVEDARSSQALALYALATEHTLRRPCTQVE
                     LHHLPSREVLAWRHDRASLREHVRRAEETAADLAAASDELVAGGDAESIFPPRPGPRC
                     STCHVRRNCPEGRAAAPERTSWALLAP"
     gene            complement(8405..9310)
                     /locus_tag="VFE39_12075"
     CDS             complement(8405..9310)
                     /locus_tag="VFE39_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HET6259379.1"
                     /translation="MAALGAGLASGVASPRIDLHTHSTASDGTDSPAGLVRAAAAAGV
                     DVLAITDHDTTGGWAEAAAALPERMSLVRGAEFSCVSPTGRGDRDVAVHLLGYLFDPD
                     HVAIVDEQARLRAERVERLRKMIEKMAADGYPVDVETVFGLLPPGASAGRPHLARALV
                     AAGVVGSVSEAFATMLYNGSPYYVPKTDTPVRAAIDMVRAAGGVVVFAHPLARKRGRV
                     VEPSVIVELAAAGLAGVEVDHPDHSAEDRDLLRGLAADAGLVATGSSDYHGSNKTTPI
                     AAEATSEAAFGELFSAATGAEVLTG"
     gene            complement(9298..9834)
                     /locus_tag="VFE39_12080"
     CDS             complement(9298..9834)
                     /locus_tag="VFE39_12080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET6259380.1"
                     /translation="MLAPREIDEYLLPTERRVIRVRQHWAFMWKHVTQTALFLLLMVL
                     LERYLPKGVLIDNLVFYMALVAVLRFTVLTILWWIERIVITDKRVMLARGIVVHNVGM
                     MPLSKVTDLTFQRTLGGRMLGYGTLIVESAGQIQALNRIDYMPRPEEIYEALSELVFG
                     EKGKTRATGMLARGRWRR"
     gene            complement(9839..10030)
                     /locus_tag="VFE39_12085"
     CDS             complement(9839..10030)
                     /locus_tag="VFE39_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259381.1"
                     /translation="MSPYARTVGVVNVKKLVGFLAIALLVFYVFTQPDSAAGNIQSIG
                     TTLRNGAESVIRFLNQLVV"
     gene            10101..10496
                     /locus_tag="VFE39_12090"
     CDS             10101..10496
                     /locus_tag="VFE39_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6259382.1"
                     /translation="MIQRGEHTPCVGALTFDEGGRLLLVRRANDPGRGLWSLPGGRVE
                     PGEDDATALVREMAEETGLVVRPGPLVGRVLRGRYAIADYRCTVIGGELRAGDDALDA
                     RWCDAAALAELPLVEHLVETLTEWDVLPR"
     gene            complement(10534..11037)
                     /locus_tag="VFE39_12095"
     CDS             complement(10534..11037)
                     /locus_tag="VFE39_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET6259383.1"
                     /translation="MQFGRYYEEFEVGAVYKHWPGKTVTEYDDHLFCLLTMNHHPLHM
                     DAHYAAETTEFGRNVVVGNYIYSLLLGMSVPDVSGKAIANLEVESLRHTKPTFHGDTI
                     YGETEVLGKTESRSKDDRGVVHVETRGYKQDGTVVCTFRRKVMVPKRSYGDARGGEQP
                     GRPVPQT"
     gene            11363..12406
                     /gene="corA"
                     /locus_tag="VFE39_12100"
     CDS             11363..12406
                     /gene="corA"
                     /locus_tag="VFE39_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009072950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HET6259384.1"
                     /translation="MKSSDSLLPKLRVPISAYVVDCAVYVEGGRLPGHWSHDRALAEV
                     RRRDDAFVWIGLHEPDEEQITGIADVFGLHELAVEDAVQAHQRPKLERYDDTLFMVLK
                     TVCYMGKPQPSAENEIVETGEVMVFLGSDFVITVRHGEHSSLRDVRNELEADPEQLAL
                     GPAAVLHAIADHIVDTYLGVTEAIENDIDEIEAEVFAPRSAVDSEQIYVMKREVLELR
                     RAVVPLGAPVRKLTEGYSSLVPHEVRSYFRDVDDHLVTVTERIAGFNELLTTLVDAVL
                     AKITMRQNNDMRKITAYVAIISVPTMIAGIYGMNFDHMPELHWRYGYPLIWLVIVVIC
                     AGLFRAFRRNEWL"
     gene            12505..13491
                     /locus_tag="VFE39_12105"
     CDS             12505..13491
                     /locus_tag="VFE39_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5914 domain-containing protein"
                     /protein_id="HET6259385.1"
                     /translation="MNELRPVNPLRRLHRPRWAEQQPTWADATPGLIGAALRRAQDRS
                     PGGWFVLAGSREIRPGRAFGRVVAGRELVAWRDDDGMLYVGPGACPHLGAALCDAPVH
                     NGQLVCRWHGLALGPDGRPGWRPVPAHDDGVLAWVRLDDLADGRPSDAPVLGPRPTGR
                     DLAAVATVIGRCEPEDVIANRLDPWHGAWFHPYSFTALRVLSAPPIDCPPAEDRFLVE
                     VTFTVGRRFGVPVRAEFSCPDARTITMEIVDGEGTGSVVETHATPLRTGQDGLPRTAV
                     IEAVLAHSDRPGFGHARRVAGALRPLVGVAARRLWRDDIAYAERRYALRAHF"
     gene            complement(13567..13731)
                     /locus_tag="VFE39_12110"
     CDS             complement(13567..13731)
                     /locus_tag="VFE39_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259386.1"
                     /translation="MALLEPLTAAVLAALLLDDRLGAVGVTGAVLLGAAVILAGRGNR
                     DRPVPERSNA"
     gene            complement(14024..14866)
                     /locus_tag="VFE39_12115"
     CDS             complement(14024..14866)
                     /locus_tag="VFE39_12115"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6259387.1"
                     /translation="MSPSSKPGPVERAGVPRSFDAHARTYDKLVAANPGYHEHLRMTV
                     RRMGLPNRGAGLRLLDLGCGTGASTAALLDGAPEAQITGVDASAEMLAQARRKTWPTT
                     VRFVHGNVENLAMNGIVGPYDGILAAYLVRNLVHRDAALRQFHRLLRPGAPIAVHEYS
                     VRDSLRSRAVWTAICWGVIIPMGKIRTGSAGLYRYLWRSVLQFDGVGALTERLAAAGF
                     DDVRVQTVPGWQQHIVHTFLGRRPATDEPARIALRSVDAPDVDPDAMTPPEGVDMTEE
                     PTGT"
     gene            15000..15842
                     /locus_tag="VFE39_12120"
     CDS             15000..15842
                     /locus_tag="VFE39_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HET6259388.1"
                     /translation="MPATGFPAADAENDFLRVRRKQVLSRLAAWLRREPDDVSEILPF
                     DEVVAALGRTGERRLGLQVVPLESIVGSVDRSRDFDRWFRPRSQRTRDRWERLARAQR
                     RGETIPPIDVYRVGELHFVRDGHHRVSVAHALGLRSIDAYVTEVTTKIDATGIARRGD
                     LITKDLRRVFLDRVPLPGPSLCTILVTDAWSYAELSQAVEAWGFRLMQQENRYLDRET
                     VARRWYSEEYRPVVRMLEQAGLIGTRTEAEAYLRLATQRYRLMRTHRWDDQVVNRLRA
                     DPGP"
     gene            complement(15864..17081)
                     /gene="ugpC"
                     /locus_tag="VFE39_12125"
     CDS             complement(15864..17081)
                     /gene="ugpC"
                     /locus_tag="VFE39_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET6259389.1"
                     /translation="MADIVLDHITKRYPDGTIAADDINVEIADGEFIILVGPSGCGKS
                     TALNMIAGLEDISEGELRIGGERMNERAPKDRDIAMVFQSYALYPHMTVRENMGFPLK
                     MAKVDKAEINRKVEEAATILDLTQHLDRRPANMSGGQRQRVAMGRAIVRSPKAFLMDE
                     PLSNLDAKLRVQMRTMVSKLQQRLQTTTVYVTHDQTEAMTLGDRIVVMRGGIVQQVGS
                     PQTLYDTPANLFVAGFIGSPAMNFLPAKLEGGTFRTPLGDLALTDELRRTLEDADVQR
                     EVIVGIRPEHFEDAALVEEHQRGRGSVFTAQVEVLESMGSDKFAYFSIEGEQAMSDDL
                     ADLAADAGTADVPGGDTGTLVTRLSATSGVREGERTEVWVDTAKIRVFDPSSGKNLTS
                     GTGAATAGETVRA"
     gene            complement(17088..17927)
                     /locus_tag="VFE39_12130"
     CDS             complement(17088..17927)
                     /locus_tag="VFE39_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6259390.1"
                     /translation="MATTTGDPRTRVWWGIAGLLVVLYALVPVLWILSLSFKTTDTIT
                     DGNFIPRSWTLDNYAQIFSLDIFTSALVNSIGIAIISTVLAVVIGTMAAYAIARLDFP
                     GKKVLVGVSLLIAMFPQISLVSPLFDIERAVGLFDTWPGLILPYITFALPLAIYTLSA
                     FFREIPWELEKAAKMDGATPMQAFRSIIVPLAAPGVFTTAILVFIFCWNDFLFAISLT
                     STSRSQTATVAIANFTGASQFAEPTGSIAAAAVLLTIPIIIFVLLFQRRIVAGLTSGA
                     VKG"
     gene            complement(17930..18892)
                     /locus_tag="VFE39_12135"
     CDS             complement(17930..18892)
                     /locus_tag="VFE39_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6259391.1"
                     /translation="MSIQSGALAAATDQASRRPAGPTKAQLSEGKRAERNLGLLLCAP
                     AAIIIVLVTAYPIGYAIWLSLQRYDLRFPAEQTFVGLSNYATVLASGYWWQAFGVTML
                     IMVVSVVIEFVLGLALALLMHRTLVARGLVRTVILIPYGIVTVVAAFSWQFAWTPDKG
                     YLAALLPTGSAPLTDFGSALAIIIGAEVWKTTPFMALLLLAGLALVPDDLLKAAQVDG
                     AGPWQRLTQIILPMMKPAILVALLFRTLDAFRVFDNIYILTQGSNGTGSVSMLGYDNL
                     FKAFNLGIGSAISVLIFICVALLAILFIKLFGASAPGSEPTGRR"
     gene            complement(18889..>19410)
                     /locus_tag="VFE39_12140"
     CDS             complement(18889..>19410)
                     /locus_tag="VFE39_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6259392.1"
                     /translation="ASIHTPPPDGGGGGTFIDAQGKPSGTDTGRRVVDEFAWAPYPSV
                     VPGQPARPTIGGINLAVSATGDHQDLAFEAIQCLRNRDNQLRNAVEGGVPPTLADLYS
                     DPAFQAKYPAWEAIKNSLDNASVRPLTPAYTSISIVLSDLLNPPAEINPDTAVPVLVD
                     QVEGAANSEGLVP"
BASE COUNT         2890 a         6949 c         6823 g         2748 t
ORIGIN      
        1 ccgcatcagc tcctcgcggg cgtagcggac gtgccgggcc tcctccgtca catggatctt
       61 gttgaccatc ctggtgagcg gttgcaccgt ctcgtcgcgc atggcctcgc gctgcagctg
      121 gtcgaggatc tcctcgacga acagcgtgcc ggcgaacatg gcgggcccgg tggcgatggt
      181 cttgaacagc ctgccgagct cgtgggtcat gcggcggggc gcgtagtcgg gcaccccgta
      241 gcgctcggcc atcttcgcga acatgaccga gtgccggcac tcgtcggcga tctcgaccag
      301 cgcgtactgg atgtgcggac ggcgcgggtc gcggtcgtag gcataccgca gcaacatctg
      361 catgaggatc atctcgaacc agatgcccat gcgggcgatg ctgcagagct cgtgcaccga
      421 cagcgtgcgc cgctgctcct cgctgaggcc ctcccacagc tcggtcccgt acagcgacac
      481 ccggtgcgcc gggatgcccc aggcgccctc gacgagcggg gcctcccagt cgacgtcgat
      541 gctcggctcg tacgagtggt ccagcgatga gcggagcagc cgctcggctg tggcctcccg
      601 gtcgctgagg gtcttgacgt tcacgatgcg ctcctctcct tgatgggcgc ggggtcggtg
      661 ccacggccgg tcagcctgcg tacgaggccg gcggcgatcg gggagccgtc gaagtggccc
      721 gcggcggcgc ggtggcggat gcgccgggcg acggcggacc cgacgaggcc gggcaggtgc
      781 cgccctgccc acagctcgat cgcccccctg cgggtgcagg cggtgtcccg gggcggcgtg
      841 gccgtggtga gtgcgcgcag caccgtggcg accacgtcgt cgacctcctc gcggcgcacc
      901 tggccctcca gcgccaggcc gagctcgcgg ccggcgtcgt ggatgggagt gcggacgtag
      961 ccgggataga cggtggtgac atgcagttcg ctgccgtact cgaggcgcag cgcgtcggcg
     1021 tacgcggtca tcccgcgctt ggccacgctg taggcagaca cgaacggaag cgtcaggtag
     1081 gccagctcgc tggagacgaa cacgacccgg cccttgcgcc ccagacgccg ggcgcgggcg
     1141 tccatcagcg ccggcagcgc ggctgcggtg acgcgccacg cgcccagcag gttgacgtcg
     1201 atgacacgca gggcgtcggg ggccagcgcg ccgccggcgt cgttcgggat gccgatgccc
     1261 gcgtagtgca ccaccgcgtc caggccaccc agccgccgca cggcctcggc gacggcccgg
     1321 gagaccgcag cgtcatcggt gatgtcgcac gcgatggtgt gctcggcagc ggccacgtcg
     1381 agcccgacga cctgcacacc gcgctcgagc aggcgggcca cggtcggggc gccgaaacca
     1441 cctgcggcac cggtgacgag gacccgcgtg ccggccatgg tgccggtcgc cgtccccgcg
     1501 gacgtcacgg tacgggtcat cggttccccc ttgccgcttg ttgttaccgg cggtaactgt
     1561 taccactggt acggtgccgc gcgacgcccc tcctgtcaag gccggacccc cgtccgcccg
     1621 gcagctcagc cgcgcggaac gccgggacga gcgccgggac gagatggtgc aggcggccat
     1681 cgacgccgtc cgcacgcacg gccccggagt gtccgtggcc gagatcgccg cgcgggcagg
     1741 catcacgaaa ccagtgctct accgccattt catcgaccgc gccgacctgc agcgcgccgt
     1801 cggccgcgag gcggtcgcgt tgttgatggg acggatggcg cccgagctgg atccgaagcg
     1861 cgagccggca gagctgatcc gcggggtcgt cgacgcgttc ctcgccggca tcgaggacga
     1921 gcccgagctc tggcggttcg tcgtgcacca ccccatcgaa cgcgcgccgg gggccgaggt
     1981 cgtcgacgac gcccgcgagc agatcgcgcg gctcctggcc actctcatcg gggagcggct
     2041 gcgcagcctc ggcctcgact ccggcggcgc ggaggcgtgg gcacagggac tggtcgggat
     2101 ggtggagagc gcaggcgact ggtggctgga gcggcgcacc atgagcaggg gggcgctcac
     2161 cgactacctg acgacgttga tctggggcgg cgtgtccggc gtgctcggcg ggacgcccgc
     2221 cgcgccggcg tcgctgcggc tcatcgagcg tgatgccgga gggcaccatg gatgacgacc
     2281 acacggaggg ctaccgaggc cccgcgacgc tcaccgtcga tggccgcgac ctgccgatac
     2341 aggtggtcct ggacgcccgg cacgaaccac tggacggccg gctgcactgg ttcgggaggg
     2401 tcgtcctgga cgacggggcc gggcccgagc tgtacacggc gctcaccgcg tcgacgagcc
     2461 gcatcgggtt gagcaccggc gccgccacgg ccgaggccag gatcggcgac gtcgacccgt
     2521 ggggccgcta ccgcgtgacc ggagtcggcg tacccccgtt cccgatcgag ccacccatcg
     2581 aggacgactg accgcacaca cctgacggcc accgcacaca cgtcaggcga tgtgcgcggt
     2641 gaccacaaga tgtgcgcggc cggtagggcg gccgagctca gccgatggcg aagcccaccc
     2701 ggcctgcggt cgcgggcgcg atctcgacgt aggcgatctg cgatgcgcgc accaagtagc
     2761 gcctgccctt gtcgtcgacc agcttgagca gcccgtcgtc ggtcttcagc gcgtcgtcga
     2821 ccagagcctg gacctcgtcc ggcgtctggt cgctggacac cacgagctcg cgcgcgctct
     2881 ccgcgatgcc gatcttgacc tccaccggtt gacctcctgg tcacgttgtc gaactggccc
     2941 ggcccaggct agtgcagctg gacacaccga gccctgatgg gcagtccgcc ctcggcgaac
     3001 tcaggtcaac ccgagcctcg ccatgcgctt gccgtggttg gactgcaacc gcttgagcag
     3061 cgcggcgatc ccggacaggt caccggagcc ggagacgatg aactcggcca gctcgtcgcg
     3121 ctcggccacg atctgctgtg cctgggtgac ggcctcgccg agcagcctgc gcccccacag
     3181 cgcgagccgg tcgtgcacct gcctgctgct cgcgcacgcc gccctgacct cgcgttcagc
     3241 gaacgcgctg tgcccggtgt cggcgagcac ctgctgcacc agctcacccg tcgggccggg
     3301 gagccagccc ccgatctccc ggtagaagtc ggctgccaac ccgtcgccga ggtatgcctt
     3361 caccagcgac tccagccagg tccggggcgc ggtggacgcg tggtaggcgt cccactgcgc
     3421 cacgaacgga cgcatggcgt cctcgacgga gacgtccaac ccccgcaggt gctcctcgag
     3481 catccggaag tgccctatct cggcggccgc cattgccgaa agggcggccc ggccgctgag
     3541 ggacggcgcg gtcctcgcgt cctcggcgag ccggtcgaac gcggacagct cgccgtacgc
     3601 cagcacgccg agcaggtcga cggtcgcccg ccccgctccg gcaggatccg ccgaccgcgc
     3661 agtcaccata ggacgagcct agaggtcacg cgacgccgcg gggcggtgca catcaccttg
     3721 tcgacatgcg cgcggggctg accggggacc gggcaccggg taggatgtcg gacagcgcag
     3781 cgcccgcacg ggtgcgcgcc tacccgcggt caccgaccgg agcccccaca gagggggtac
     3841 aggatcggag ccgcaacgac ctggtgcgtg cagcgccttg tcggttctcc cacctgggcg
     3901 cgccgtgacc tccacggaac agggcagccg gtggtcgacg agccggcccc ggctcctgtg
     3961 cgcggagaga ggcgatcatc ctgtcggttc agagcgacac atccaccgag tcccccgtgg
     4021 actccccaga caactccaca gacgcagccg tcgacgagat cgtcgaggcg cacccgctgc
     4081 aggccggcgc ccccgttgcg ccggactccc ccaccttcgc cgagctcggg gtgcgcccgg
     4141 agatcgtgcg agcgctggcc gaggccggca tcgtgcgcac gttcgcgatc caggagctca
     4201 cgctcccgct ggcgctggcc ggcgacgaca tcatcggcca ggcccgcacc ggcatgggca
     4261 agacgctcgg cttcggagtc ccgctgctgc agcgcgtcac tccgccgtcc gagcagcccg
     4321 cggccaacgc cgacggcgag tcggccgacc gcaccaagga cgtgccgcag gcgctcgtcg
     4381 tggtgccgac ccgcgagctg tgtgtgcagg tcgccaagga catcgccgac gccggcaagc
     4441 acctcggcgt ccgtgtcacc gcgatctacg gcgggcgcgc ctacgagccg cagctggccg
     4501 ccctgcgtaa gggcgtcgac gtcgtggtgg gcactcccgg ccgcctgctc gacctcgccg
     4561 agcaacgcca cctcgtactg ggccgtgtca aggccctcgt gctcgacgag gccgacgaga
     4621 tgctggacct gggcttcctg cccgacgtcg agcggatcat gcggatgctg cccgagaagc
     4681 ggcacaccat gctgttctcg gccaccatgc ccggcccgat catcgcgctg tcgcgggcct
     4741 tcctgaaccg gcccacgcac atccgcgcgg aggaggccga ccagggttcg atccacgagc
     4801 gcacccggca gctggtctac cgcgcccacg ccatggacaa ggtcgagctg ctcacccgcg
     4861 tgctccaggc cgatgagcgc gggctcacca tgatcttcgc gcgcaccaag cgcaccgtgc
     4921 agcgcgtggc cgacgacctg gccgagcgcg gattcgcggc cgcggctgtg cacggcgacc
     4981 tcggtcaggg cgcccgcgag caggcgctgc gcgcgttccg ctccggcaag gtcgacgtgc
     5041 tggtggccac cgacgtcgcg gctcgcggta tcgacgtcac cgacgtcacg cacgtggtca
     5101 actaccagtg cccggaggac gccaagacct acgtccaccg gatcggccgc accgggcgcg
     5161 caggcaagga gggcgtcgcc gtcacgctgg tcgactggga cgagatgccg cgctggaaga
     5221 tgatcagcga cgacctcggt ctcgacatgc ccgagcccgt cgagacctac tcgacgtccg
     5281 agcacctgta ctccgacctc gccatcccga ccggatcgac cgggcggctg cctcgctccc
     5341 agcgcacccg cgcggggctg gacgcggagt acgtcgacac cgagggcgat cacggcggcc
     5401 gcaagcgggg cgacaagtcc tcccgcgccg gccgcgtcgg cggtgcacgg tccaccggcg
     5461 ccgcacgcaa tggcgacgag gacgccgagc ggtcgcgccc gcgtcgcacc cgtgcccgca
     5521 cgcgcagccg gtccggcgtg acggtcggtg gcggcgacgc aaccggcgcc gagacggcca
     5581 ccgactcggc tcccaccgcc tcgacgagcg gcaccgtcga tgccgccact accaccgaca
     5641 ggcccgacag tggtcccgac acgggagcgg ggtcgcgcgc cgagggagac cggccgcgcc
     5701 ggcgtcgtcg gcgtggaggc acccgccgcc gcggtgccga gggcaacgac ggcgagggcg
     5761 gcgagcagca gtccgcgccg gacgccgccg ccagctgacc agcacccggt gcgtctcgag
     5821 ccgagccggc ggctgcggtc accgcagccg cggcgcttgc gcttaccgcc cgagcggcgc
     5881 cgccgcagcg acatcgtcac tgcggtggtg ctggcgctgc tgctgatcgg ggccggtctc
     5941 accctgtggc gcaccagccc cgtcgcccac accgcggatg atccggcggc catcccgatc
     6001 gcgccgccgc ctgcggctac ggatgttccc gccgggttcg ccgaggcatg gcgggcgccc
     6061 agcggcgcca ccgaccagcc cgtcgtggcc ggtccggcgg ctgtcaccgc cgacggctcc
     6121 cgcgtcgtcg gccgcgacgc ggccaccggc gccgagcgct ggagctacgc ccgggaccgg
     6181 ccgctgtgca ctgtcgactc tgcgttcccc gatggcggag tcaggcgcat cctcgctctc
     6241 tacgccggca ccaccgggta ctgcagcgag ctgacggcgc tgcgctcgga caccgggacg
     6301 cggctcgccg cgagcaatcc cgacgtccgg cccggcacac gactcctggc ggatcgcacg
     6361 tacgtggtcg cgaccggtgc cgactacctg gaggtctggc gcagcgacct cgtcaagacc
     6421 ctcgagtacg gcgccgtcac cacacccctg caggcggacc ggcagccgcg ggcgggctgc
     6481 acctacggct cggtggcggc cagcggagga cgcgtcgggg tcatcgagcg ctgcccgggc
     6541 gaggccaccg accggctcac ggtgctcgcc gccgacggcg aggacggtgc tgacacgccg
     6601 caggtggagt tctcggtccc gctgccctca gccggcgcaa cgctggtcgc tctctcggcg
     6661 gaccgggccg cggtggcgtt gccggggccg ccacggctgg aactgctcga cggggccgga
     6721 caacaggtcg gcctcgtccc cctcgacgta ccggacgccg accttgcgac cgacgcgccc
     6781 ggcaggatcg cgacgaccga gtccaccgtg ggcagcgcga acgggacggg cgccggagac
     6841 ggccgcgtct actggtggac cggctcccgg acgatcgcgc tcgacggcac cgacctcgcc
     6901 ccactctgga cggtcgacgg caccctcgga cccgcttttc cctatggcgg gggcctcctc
     6961 gtgcctgtcc cggccgggct cctggacgtc gacgccgccc gcggcaccgc gctgcgaacc
     7021 cttcccgtcg cccgcgccga tccgacggcg ccggtccggc tcgcggccgt gggcaacgtg
     7081 ctgctcgagc aacgcggcac ggaggtggtc gccctgcgcc cagccggctg accggggctc
     7141 gcgggctcgg ggcgggcgca ggcggagcac ccggggacca ccgtcaggcc aaccactcgg
     7201 cggtcagcag tgccaccgtg atcgcgatca cgatacaggc ggtcatggcc ccggccgcgc
     7261 cccggtgacg gtcggcgtgc cgctgagcca gcaccgccgc gacggccgcg acggcgtgcc
     7321 acgcgagcgt gcttgcgccc ggtccggacg tgccggaacg gtcagcgatg taccaggcga
     7381 tgcacagccc caccagcagc gccacgagcc cgcctgcaag caggccggac agaccgcgca
     7441 gccatccccc ggttcgccgg gcgctcacgg cgcgagcagg gcccacgagg tccgctccgg
     7501 agcggcggcc ctgccctccg gacagttgcg cctgacatgg cacgtcgagc accgaggccc
     7561 gggacgaggc gggaagatcg actcggcgtc gccgcccgcg acgagctcgt ccgaggcggc
     7621 agcgagatca gcggccgtct cctccgcccg ccggacgtgc tcgcgcagcg acgcgcggtc
     7681 gtgccgccag gccagcacct cacggctcgg caggtggtgc agctccacct gcgtgcaggg
     7741 gcggcggagg gtgtgttcgg tggcaagggc gtacagcgcg agggcctgcg aggacctcgc
     7801 gtcctcgacg gtgggggtac gcctgcccgt cttgtagtcg acgaccacca gctccgttcc
     7861 cctccggtcg atccgatcga cccgcccctc ggcgaggatc cggtcggtgg cgaccgacac
     7921 ccactgttcc agccccaccg gaccgacgtc cgactcggcc acggtgtcca tgctcgctct
     7981 gccggcgctc cactcgggat cgatgctcgc tccgccggcg ctccactcgg cggcatagtc
     8041 cgccacccac tcacccgccc gctcgcggta ctccgccgcc tgggcggcgt ctcggaaacc
     8101 ctcgctcgtc cagtgccggt tgaccagcgc cgctgccgcg cgcggggtgc gatctgcagc
     8161 cggcaggtcg aacagggagc gcagtgcgag gtggacgacg gcgccgaggg tgttgtgtgc
     8221 ccaggcccct cccctcggag gggagggccg gtccaggtag gtcatccggt accggcgggg
     8281 gcagtcggtc caggtggcca gcctcgacgg cgtcacccgt accagccggg tggtctcgaa
     8341 gtcgaaaccg agctgcgcac cgccgttcac cggcagcacc gtaaacctcg cccgccgggc
     8401 tcggtcaccc ggtgagcacc tcggcccccg tggccgcgga gaacagctcc ccgaaggcgg
     8461 cctcgctcgt ggcctcggcc gcgatcgggg tggtcttgtt gctcccgtga tagtcgctgg
     8521 agccggtggc caccagtccg gcatccgcgg cgagtccgcg cagcaggtcg cggtcctcgg
     8581 cggaatggtc gggatggtcc acctccacac cggcgagccc ggctgccgcg agctcgacga
     8641 tcaccgacgg ctccaccacc cgtccccgct tccgggccag cgggtgggcg aacaccacca
     8701 cgccgcccgc ggcgcgcacc atgtcgatcg cggcacggac cggcgtgtcg gtcttcggca
     8761 cgtagtacgg gctgccgttg tagagcatcg tcgcgaacgc ctcgctcacc gacccgacca
     8821 cgcccgccgc cacgagtgca cgcgccaggt gcggccttcc ggcgctcgcc ccgggcggga
     8881 gcagcccgaa gacggtctcg acgtcgacgg ggtagccgtc ggccgccatc ttctcgatca
     8941 tcttgcgcag ccgctcgacg cgctcggccc gcagccgcgc ttgctcatcg acgatcgcga
     9001 catggtccgg gtcgaagaga tagcccagca ggtgcaccgc gacgtcccgg tcgcccctgc
     9061 ccgtggggct gacgcaggag aactccgcgc cgcggaccag cgacatccgt tcgggcagcg
     9121 cggcggccgc ctcggcccat cccccggtgg tgtcgtggtc ggtgatcgcc agaacgtcca
     9181 ccccggcggc agcagcggcc ctcacgaggc ctgcggggct gtccgtgcca tctgaggccg
     9241 tggaatgcgt atgcaggtcg atgcgaggac tggccacgcc tgaggctaac ccggcgccta
     9301 gcgccgccat cggcctcggg ccagcatgcc cgtggccctc gtcttgccct tctcgccgaa
     9361 gaccagctcg gacagggcct cgtagatctc ctcggggcga ggcatgtagt cgatccggtt
     9421 gagtgcctgg atctgaccgg cggactcgac gatcagggtg ccgtagccca gcatccgacc
     9481 accgagcgtg cgctggaacg tcaggtcggt gaccttgctc agcggcatca tgccgacgtt
     9541 gtgcacgacg atgccccggg cgagcatcac ccgcttgtcg gtgatcacga tgcgctcgat
     9601 ccaccacagg atggtcagca cggtgaaccg caacaccgcc acgagggcca tgtagaacac
     9661 gaggttgtcg atgagcacgc ccttgggcag gtagcgctcg agcagcacca tgagcagcag
     9721 gaacagggca gtctgcgtga cgtgcttcca catgaacgcc cagtgctggc gcacgcggat
     9781 gacccgacgc tcggtcggga gcaggtactc gtcgatctcg cggggcgcaa gcaccggact
     9841 acacgacgag ctggttcaag aagcggatga cggattccgc cccgttgcgc agcgtggtgc
     9901 cgatgctctg gatgttgccc gcagcgctgt ccggctgggt gaagacgtag aagacaagca
     9961 acgcgatcgc gagaaacccg acgagcttct tgacgttcac cacacccacc gttctggcgt
    10021 aaggggacac gtgcccatga tttgtaccgc acccatccgg ctgagggtat ccggcgcgcg
    10081 gggccgtatg gtcgttgacg atgatccaaa gaggtgaaca taccccgtgc gtgggggcct
    10141 tgactttcga cgagggaggc aggcttctgc tggtccgccg ggcgaacgat cccggccgcg
    10201 ggttgtggtc cctgcccgga ggccgggtgg aaccgggcga ggacgacgcc acggcgctgg
    10261 tccgggagat ggcggaggag accggcctcg tggtgcggcc cggaccattg gtcggccggg
    10321 tgctccgcgg gcgctacgcc atcgccgact accggtgcac cgtcatcggc ggcgaactcc
    10381 gtgccggcga cgatgcactc gacgcccgat ggtgcgacgc tgcggctctg gccgagctac
    10441 cgctcgtcga acacctcgtg gagacgctca cggagtggga cgtcctcccg cgctgacggc
    10501 gtgtgccggc acgccgggcc ccgccgccag acgtcaggtc tgtggcaccg gccggccggg
    10561 ctgctccccg ccgcgcgcat cgccgtagga ccgcttcggc accatcacct tccggcggaa
    10621 cgtgcagacg accgtgccgt cctgcttgta cccgcgcgtc tcgacgtgga cgacgccgcg
    10681 gtcgtccttc gacctcgact cggtcttgcc cagcacctcg gtctcgccgt agatcgtgtc
    10741 gccgtggaac gtcggcttcg tgtgcctgag cgactccacc tcgaggttgg cgatcgcctt
    10801 gcccgagacg tcgggcaccg acatccccag cagcagcgag tagatgtagt tgccgaccac
    10861 gacgttgcgc ccgaactccg tggtctcggc cgcgtagtgc gcgtccatgt gcagcgggtg
    10921 gtggttcatg gtgagcaggc agaagagatg gtcgtcgtac tcggtcaccg tcttgcccgg
    10981 ccagtgcttg tagaccgctc cgacctcgaa ctcctcgtag taccggccga actgcaacgc
    11041 cgccgcctcc atcgtcgata accaggtggg agtaatctag tggtcggacc ggccacgaac
    11101 ttccactggc gggtctctga gtgcttgcca cgtaccgcgt ggccacgagc acaggacgag
    11161 gaggtgagcg cggtgccgac ggcaccctgt tgcagtagtc gccgcgttct cggcacggcg
    11221 acctcgtaac ggtctccccg gtcgggacgc gagctccacg cccgcgcgat gagcagtgct
    11281 ccacgcgcgt cccgaactcc ggaggtacct cgtgccgccc ctttcctcgc tccggcctgc
    11341 catccgcgcc ggtcgactgt ccgtgaagag cagcgattcg ctgctgccca agttgcgcgt
    11401 cccgatctcg gcctacgtcg tcgactgtgc ggtctacgtg gagggtgggc gcctgccggg
    11461 tcactggtcc cacgaccgcg cgctcgccga ggtgcgccgc cgcgacgacg ctttcgtctg
    11521 gatcgggctg cacgagcccg acgaggaaca gatcaccggg atcgccgacg tcttcggcct
    11581 gcacgagctc gccgtcgagg acgccgtgca ggcccaccag cgcccgaagc tggagcgcta
    11641 cgacgacacg ctcttcatgg tgctcaagac ggtctgctac atggggaagc cgcagccctc
    11701 ggcggagaac gagatcgtcg agaccggcga ggtgatggtg ttcctgggct ccgacttcgt
    11761 gatcacggtg cggcacggcg agcactcctc gctccgggac gtgcgcaacg agctggaggc
    11821 cgaccccgag cagctcgctc tggggccggc agcggtgctg cacgcgatcg ccgaccacat
    11881 cgtcgacacg tatctcggcg tcaccgaggc gatcgagaac gacatcgacg agatagaggc
    11941 cgaggtcttc gcaccgcggt ccgcggtcga ctccgagcag atctacgtga tgaagcgcga
    12001 agtgctcgag ctgcgccgcg cggtcgtccc gctgggtgcg ccggtacgca agctcacgga
    12061 gggctacagc tcgctggtgc cccacgaggt gcggtcgtac ttccgggacg tcgacgacca
    12121 cctggtcacg gtcacggagc ggatcgcggg cttcaacgag ctgttgacca cgctggtcga
    12181 cgccgtgctg gcgaagatca cgatgcggca gaacaacgac atgcgcaaga tcaccgccta
    12241 cgtcgcgatc atctccgtcc ctacgatgat cgcgggcatc tacgggatga acttcgacca
    12301 catgcccgag ctgcactggc gctacggcta tccgctcata tggctggtga tcgtcgtgat
    12361 ctgcgcgggg ctcttccggg cgttccgacg caacgagtgg ctctgacgcc caccgcgcag
    12421 cagctcgtgg cgccgcgccg cgccacccga ccgggtcggg cgatcgaccg gcgggtcggg
    12481 cgcggccgtc ctagccttga cggcgtgaac gagctccgtc cggtcaaccc gctgcgaagg
    12541 ctgcaccgcc ctcgctgggc cgagcagcag ccgacatggg ccgacgcgac acccgggctc
    12601 atcggcgcag cgttgcggcg ggcgcaggac cggtcgcccg gcgggtggtt cgtgctggcc
    12661 gggagccgcg agatccggcc gggccgggcg ttcgggcgcg tggtcgccgg ccgggagctc
    12721 gtggcatggc gcgacgacga cggcatgctg tacgtcgggc cgggcgcgtg cccccacctc
    12781 ggcgcagccc tgtgtgatgc ccccgtgcac aacgggcaac tcgtgtgccg gtggcacggc
    12841 ctggcgctcg ggccggacgg gcggcccggc tggcggccgg tgccggcaca cgacgacggc
    12901 gtgctcgcct gggtgcgtct cgacgacctc gcagacggtc gcccgtccga cgccccggtg
    12961 ctcggccctc gccccacggg ccgggatctc gccgccgtcg ccacggtgat cgggcggtgt
    13021 gagccggagg acgtcatcgc caacaggctt gacccgtggc acggcgcctg gttccacccg
    13081 tactcgttca cagcgcttcg ggtgctgagc gcaccaccga tcgactgccc cccggcggag
    13141 gaccgctttc tcgtcgaagt gacgttcacg gtcggccggc gcttcggggt gccggtacgg
    13201 gcggagttca gctgccccga cgcgcggacg atcaccatgg agatcgtcga tggcgagggc
    13261 accggcagcg tcgtcgaaac ccacgccacg ccgctgcgaa cgggccagga cggtctgccc
    13321 cgcaccgcag tcatcgaggc cgtgctggcc cactccgacc gtccgggatt cggccacgcg
    13381 cgacgtgtcg ccggtgcact gcgcccgctc gtcggtgtgg ccgcccggcg gctctggcgc
    13441 gacgacatcg cctacgccga gcgccgctac gcgctacgcg cccacttctg agccgctgcc
    13501 ccaacgcttc aggatgacgt agagccgggg gcacaatcgc catgcccccg ggcacaacgg
    13561 cgtggcctac gcgttgctgc gttccgggac ggggcgatcc cggttccccc ggcccgcgag
    13621 gatgaccgcg gccccgagga gcaccgcacc ggtgacaccg acggcgccca gccggtcgtc
    13681 gagcagcagc gcggcgagca ctgcagcggt caatggctcc aacagcgcca tcaccacacc
    13741 gaccgtcgcc gacgatgagc gcagccgccc cagaggactc cagccagaac gaggaagggc
    13801 accccggaga cccgggtggt tcggacgatg gacatgggtg ttctgcgctc ctgcgacggg
    13861 ggaaaggggg gtcgcggaag cggggccggt ccgagcgcac gcgagacgtg cggcaccggt
    13921 ggtcgaagga cgatctggcg cgcacctcgc gctgatcagg cgagtgcgca ggcggccccg
    13981 cccctagtcg accggcctcc tacgggcacg cgggttcaag aaatcacgtc cctgtgggct
    14041 cctcggtcat gtcgacgccc tcgggcgggg tcatcgcgtc gggatcgaca tccggggcgt
    14101 cgacactcct cagcgcgatc cgtgccggct cgtcagtggc gggtctgcgg ccgaggaagg
    14161 tgtgcacgat gtgctgctgc cagcccggga cggtctgtac ccgcacgtcg tcgaacccgg
    14221 ccgcggccaa ccgttcggtg agtgcaccca cgccgtcgaa ctgcaggaca ctgcgccaga
    14281 ggtaccggta caggccggcg gagcccgtgc ggatcttccc catcgggatg atcacgcccc
    14341 agcagatcgc agtccagacc gcacggctgc ggagcgagtc gcggaccgag tactcgtgca
    14401 ccgcgatcgg cgcgccgggc cgcagcaggc ggtggaactg gcgcagtgcg gcgtcgcgat
    14461 gcacgaggtt gcgcacgagg taggccgcga ggatgccgtc gtaggggccg acgatcccgt
    14521 tcatcgccag gttctcgacg ttgccgtgca cgaaccggac ggtggtcggc cacgtcttgc
    14581 gacgggcctg cgccagcatc tccgccgacg cgtccactcc ggtgatctgg gcctccggag
    14641 caccgtcgag aagcgcggcc gtggacgcac cggtgccaca gcccaggtcg agcagccgaa
    14701 gcccggcccc ccggttcggc aggcccatcc gtctcaccgt catccggaga tgctcgtgat
    14761 agccggggtt ggccgcaacg agcttgtcgt aggtgcgggc gtgggcgtcg aacgagcgcg
    14821 gcacgcctgc acgctcgacc gggcccggct tggacgaggg ggacacgctc tgcatcctgt
    14881 cgcatccggg gccatctcgt cgccatgaca cgtaccctga ccccggtggt ccggggcccc
    14941 gcgcggtgag cgacgaggtc gaggccgggc agcagcgacc ccgactcgag gaggggtgaa
    15001 tgcccgccac cgggttcccc gccgccgatg ccgagaacga cttcctccgg gtccgccgca
    15061 agcaggtgct ctcccggctc gccgcgtggc tgcggcgcga gcctgacgac gtctccgaga
    15121 tcctgccctt cgacgaggtc gtggccgcac tgggccgcac gggggaacgc cgcctcgggc
    15181 tgcaggtcgt cccgctggag tcgatcgtcg ggagcgtcga ccgctcacgc gacttcgacc
    15241 gctggttccg gccgcgctca caacgcacgc gggaccggtg ggagcggctg gcgcgggcgc
    15301 agcggcgggg cgagaccatc cccccgatcg acgtctaccg ggtgggtgag ctgcacttcg
    15361 tgcgcgatgg ccaccaccgc gtctcggtgg cgcacgcact ggggctccgg tcgatcgacg
    15421 cgtacgtcac cgaggtgacc accaagatcg acgcaaccgg aatcgccagg cgcggcgacc
    15481 tgatcaccaa ggacctgcgc cgggtgttcc tcgatcgcgt ccccctgccc ggcccctcgc
    15541 tgtgcacgat cctcgtcacc gacgcgtggt cctacgccga gctcagtcag gccgtcgagg
    15601 cctggggctt ccggctgatg cagcaggaga accgctacct cgaccgcgag acggtggccc
    15661 ggcgctggta ctccgaggag taccgcccgg tggtacgcat gctcgagcag gccggcctga
    15721 tcggcacccg cacggaggcc gaggcctatc tccggctggc cacacagcgc taccgcctga
    15781 tgcgcaccca ccgctgggac gaccaggtgg tcaaccgcct gcgggccgac ccgggcccct
    15841 gaccgggtcg acccgcaggg tccctaggcg cgcaccgtct ccccggccgt tgcggccccc
    15901 gttcccgagg tcaggttctt cccgctcgac gggtcgaaga ccctgatctt ggcggtgtcc
    15961 acccagacct ccgtccgctc gccctcgcgc acgcccgatg tggcggacag gcgcgtgacc
    16021 agcgtgccgg tgtctccccc tggcacgtcg gcggtgccgg catcggccgc caggtcggcc
    16081 agatcgtcgg acatcgcctg ctcgccctcg atgctgaagt aggcgaactt gtccgagccc
    16141 atcgactcca gtacctccac ctgggcggtg aacaccgacc cgcggccccg ctggtgctcc
    16201 tcgacgagcg ccgcgtcctc gaagtgctcc ggccggatgc ccacgatcac ctcgcgctgc
    16261 acgtcggcgt cctcgagcgt gcggcgcagc tcgtcggtga gggcgagatc gcccagtggg
    16321 gtgcggaacg tgccaccctc gagcttcgca ggcaggaagt tcatggcggg cgagccgatg
    16381 aacccggcga cgaagaggtt ggccggggtg tcgtagagag tctgcggcga accgacctgc
    16441 tgcacgatcc cgccacgcat gacaacgatg cggtcgccga gtgtcatggc ctcggtctgg
    16501 tcatgcgtga cgtacacggt ggtggtctgc agccgctgct ggagcttgga gaccatcgtg
    16561 cgcatctgca cgcgcagctt ggcgtcgagg ttggacagcg gctcgtccat caggaacgcc
    16621 ttggggctac ggacgatggc gcggcccatc gccacccgct ggcgctgacc accggacatg
    16681 ttcgccggcc tgcggtcgag gtgctgagtg aggtcgagga tcgtcgcagc ctcctcgacc
    16741 ttgcggttga tctcggcctt gtccaccttg gccatcttga gcgggaagcc catgttctcc
    16801 cggacggtca tgtgcggata gagcgcgtag gactggaaca ccatggcgat gtcgcggtcc
    16861 ttgggcgccc gctcgttcat ccgctcgccg ccgatccgca gctcgccctc gctgatgtcc
    16921 tccagcccgg cgatcatgtt gagcgccgtg gacttcccgc agcccgacgg cccgaccagg
    16981 atgatgaact cgccgtcggc gatctcgacg ttgatgtcgt cggccgcaat cgtgccgtcg
    17041 gggtagcgct tggtgatgtg gtccagaacg atgtcagcca cgtgatctca gcccttcact
    17101 gcaccggacg tcaggccggc gacgatgcgc cgctggaaca acagaacgaa gatgatgatc
    17161 gggatcgtga ggagcactgc ggcagccgcg atcgagccgg tgggctcggc gaactgcgac
    17221 gcgccggtga agttcgcgat cgccacggtg gcggtctgcg accgcgacgt ggacgtcagc
    17281 gagatcgcga acaggaagtc gttccagcag aagatgaaca ccaggatggc cgtggtgaac
    17341 acgccgggcg cagccagggg gacgattatc gagcggaacg cctgcatcgg cgtggccccg
    17401 tccatcttgg ccgccttctc cagctcccac gggatctcgc ggaagaacgc cgagagcgtg
    17461 tagatcgcca gcggcagcgc gaacgtgatg tagggcagga tcagcccggg ccaggtgtcg
    17521 aacagcccca ccgcgcgctc gatgtcgaac agcggcgaga ccaacgagat ctgcgggaac
    17581 atcgcgatca gcagcgacac cccgaccaga accttcttgc cggggaagtc gaggcgggcg
    17641 atggcatagg ccgccatcgt gccgatcacg acggccagca ccgtcgaaat gatcgcgatg
    17701 ccgatcgagt tcaccagtgc actggtgaag atgtcgagcg agaagatctg cgcgtagttg
    17761 tccagggtcc agctgcgcgg gatgaagttg ccgtcggtga tcgtgtcggt ggtcttgaac
    17821 gagaggctca agatccacag caccggcacc agcgcgtaga ggacgaccag caggccggcg
    17881 atcccccacc agactctcgt gcggggatcg cccgtcgtcg tcgccatcgt caacgcctcc
    17941 cggtcggctc ggagcccggc gccgacgcgc cgaacagttt gatgaacaga atggcgagca
    18001 gagcgacgca gatgaagatc agcacactga tggcggagcc gatgccgagg ttgaaggcct
    18061 tgaacagatt gtcgtaaccc agcatcgaca ccgagccggt gccgttggaa ccctgggtca
    18121 ggatgtagat gttgtcgaaa acccgaaacg cgtcgagcgt gcggaacagc agcgcgacaa
    18181 ggatcgccgg cttcatcatc ggcaggatga tctgcgtcag ccgctgccac ggccctgcgc
    18241 cgtcgacctg cgcggccttg agcaggtcgt cgggcacgag ggccagaccg gcgagcagca
    18301 gcagggccat gaacggggtg gtcttccaga cttcggcgcc gatgatgatg gccagcgcgg
    18361 agccgaagtc ggtgagcggt gcgctgccgg tgggcagcag cgccgccagg taccccttgt
    18421 ccggcgtcca ggcgaactgc cagctgaacg cggccaccac cgtgacgatc ccgtacggga
    18481 tgaggatgac cgtgcggacc aggccgcggg cgacgagcgt gcggtgcatg agcagcgcga
    18541 gggcgagccc cagcacgaac tcgatcacca ccgagacgac catgatcagc atcgtcacgc
    18601 cgaacgcctg ccaccagtag ccggacgcca gcaccgtggc gtagttgctc agccccacga
    18661 aggtctgctc ggccgggaag cggaggtcat agcgctgcag cgacagccag atcgcgtaac
    18721 cgatcgggta ggcggtgacc agcacgatga tgatcgccgc cggggcacac agcaggagtc
    18781 cgaggttccg ctccgcgcgc ttgccctccg acagctgcgc cttggtgggc cccgccgggc
    18841 gccgggacgc ctggtcggtc gccgctgcga gagcaccact ctggatactc atggcaccag
    18901 cccttccgaa ttggcggcgc cctcgacctg gtcgacgagc accgggaccg cggtgtcggg
    18961 gttgatctcc gcgggcgggt tgagcaggtc ggacagcacg atcgagatgc tcgtgtatgc
    19021 cggcgttagc ggccggacgc tcgcgttgtc gagcgagttc ttgatcgcct cccaagccgg
    19081 atacttcgcc tggaacgccg ggtcgctgta gaggtcggca agggtcgggg gcacaccgcc
    19141 ctccacggcg ttgcgcagct ggttgtcgcg gttgcgcagg cactggatgg cctcgaacgc
    19201 gaggtcctga tggtcgcccg tcgcgctgac ggcgaggttg atgccgccga tcgtgggtct
    19261 ggcgggctgc cccggtacga cgctcgggta cggcgcccag gcgaactcgt cgaccacccg
    19321 gcggccggtg tctgtgcccg acggcttgcc ctgggcgtcg atgaacgtgc cgccgccccc
    19381 accgtcgggc ggcggggtgt ggatcgaggc
//
LOCUS       DASRSC010000248        18973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_258351,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000248 DASRSC010000000
VERSION     DASRSC010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 18973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18973
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258351"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..2326
                     /locus_tag="VFE39_12145"
     CDS             62..2326
                     /locus_tag="VFE39_12145"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET6259393.1"
                     /translation="MTAVLPCSDRPPHGCAPRHRRGRRRHDPSRSRLQWILAVGRGSA
                     RARAQRTVQTAVVPVQLERRTRLPPDARSAGRARRMLQEALRPLDLGGGDGADGDLVD
                     TVVLLASELCENAVLHAGTEFEVALTVTDDEVAVAVTDRGAGPLELHLAQPRQRYGRA
                     ASHGRGLALVQRLATTWGTRHESDGRHVIWFSVGRGPAVAAPPTPPAPPDTRRVWSTA
                     EQARWLLHVPSALGDRLEPAELVAELIRRLRELLDADSVDVEVDEGDGNGPQEIARDG
                     AGRAGGTVVEIRLPMTAPLRGVLRVVHRPGAQAPGVEADRRRDLTELIASRVAMAVES
                     KWLRGVDQRRRAWMTYLAETSELLGQSLDVELAVAVVPQVVVPRLGRWCAVHLMEPSG
                     RLRLAALTHADETKLTELRGVLDPDRTPGLPSELRTRFAELVRDGTAPVRFAVPTDGI
                     ALPLRARGRTIGTLLVGRPEGRPHTPEDVVLAGDIARRAALTIHNAQSTGAHVAVSQA
                     LQQALLPRALPMAPGLDFAAEYLPASTGSDVGGDFYDVLTVDTSRWLVSIGDVCGKGA
                     RAAARTGLVRDVLRVLIRDGRPLPRAIEMLNQVMIEAGDPLQFCTIAAAMVSRRRPVA
                     IDPSPRTGHTGLDVELVLAGHVQPVLVRADGAAELIGQFGTAVGLVPSIRLECTPHRL
                     APGDTLLVYTDGVTERRRGREQFGSERLLDVATKAAGRPARQVVAAVREAVERFSADP
                     LNDDVALLAVRATP"
     gene            2353..3939
                     /locus_tag="VFE39_12150"
     CDS             2353..3939
                     /locus_tag="VFE39_12150"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET6259394.1"
                     /translation="MAARLAAVRVPLEPAVTHPGASTAAAISPHGGGALDKKPSGRTV
                     LRELRSWPTLLLVLFCIGVGVPVTVTLTPAQDLVAFGQHISVGARPPALSVAGPARLV
                     QVGNTALDVDRLHVYGPLRPQLTMGPVQRSAAAAAVFDPVEGPEVRDEAVSSVTTAFI
                     TWYVLGGLGLIAVTLAAAAGAAGLRTLVVLRRQSRETGGHAPLHEIFTYCVRAARRMT
                     AIALVASVVAWGVSGVLAYTGAVQGLRGVSSLAQLVGAYHLSPDPVGPPLSGYDGAVI
                     GDSRAVRVGGPPLPGGTPDDAACERSTDSLAAEIGHLASAKVLNLACPSATVTTGLRG
                     PQQRGDRVVPPQIGVLKQVQDLRFVVVAIGPNDVGWTDFLRYCYGVADCSDQLTQGEF
                     DYRMAAFDRVYGDLLVDLNDLPGRPQVIVMTSYGAFRPDADCADTRAAGHPGLDPAKI
                     ELLTARNEQLNAVLSAGAEKYGFAVARPELALLCEDGTDGLGPDLQGMADPFPFHPTG
                     IGSLRMASSVLRLIAPAQER"
     gene            4012..5073
                     /locus_tag="VFE39_12155"
     CDS             4012..5073
                     /locus_tag="VFE39_12155"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET6259395.1"
                     /translation="MGYWVLHVDLDQFIAAVEILRRPELAGAPVVVGGAGDPTQRAVV
                     ATASYPAREHGVRSGMPLRTAARRCPDAVFLPADNAHYEEVSEEVMATLRRIPDVVVE
                     VMGWDEAFLGVETDDPESLAREVRAAVLAATRLSCTVGIGDNLLRAKLATEFGKASPG
                     SERGPGMGTGVFCLTAANWEEVMAERPTQALWGIGTKTARKLAERGLTTVRELAAADP
                     GVLAADLGPSMGPWYVQLGRGIGRTRVEGTPWVRRSRSRETTFQENITVWDDVRTEVA
                     ALARRVARDITVDGRPAARVAVKVRFAPFSTYTRSATLDAPTYDGDRIEAGALRVLEL
                     FTSRRPVRLLGVRAEFEQP"
     gene            complement(5105..5851)
                     /locus_tag="VFE39_12160"
     CDS             complement(5105..5851)
                     /locus_tag="VFE39_12160"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005162682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HET6259396.1"
                     /translation="MKVLVLGGTGQARRLADALNERKFVVTSSLAGRVAVPSLPPGDV
                     RIGGFGGVDGLAAWLRRHGTDVVVDATHPFAVRMTVHAVAATAVAGVPLLVLRRRGWT
                     AGPGDRWHRVPDAASAAALLPTLGERVFLSTGRGDLAAFAALDGLWFLLRSVDPPQPP
                     LPARCEVVRARGPFTADAERELLREHRIDVLVTRDSGGEMTAAKLVAARELGVPVVLL
                     DRPPAPDVPAVGTVEEAVRWLEEARERGAP"
     gene            complement(5848..6615)
                     /gene="cobF"
                     /locus_tag="VFE39_12165"
     CDS             complement(5848..6615)
                     /gene="cobF"
                     /locus_tag="VFE39_12165"
                     /EC_number="2.1.1.152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009081420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043819 - precorrin-6A synthase
                     (deacetylating) activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A synthase (deacetylating)"
                     /protein_id="HET6259397.1"
                     /translation="MRRIYVIGIGAGDPQHLTLQAVEAMNRVDVFFTIDKGDDKGDLA
                     RLRIELLDRHVTRHHRVVEAGDPERDRAAAPGGYEAAVVDWQERRESIYQRMIADELD
                     EDGCGAFLVWGDPALYDGTLRILERIGARGAVGFEVESIPGISSVQALAARHRLILNR
                     VGRPFMVTTGRRLAAQGLPAGIDDVVVMLDAHNAFATLPDTDLDIYWGAYLGTADEVL
                     LHGDLQQVKGEIVRVRAEQRERKGWIMDTYLLRRRDG"
     gene            complement(6625..7674)
                     /locus_tag="VFE39_12170"
     CDS             complement(6625..7674)
                     /locus_tag="VFE39_12170"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HET6259398.1"
                     /translation="MSGDWWILLLAALPVLALLVAVAVRSRPSGTDPDSLTAEPEDRP
                     LPAVVANPTKIEPGTRRRIESVCAGLGWAEPLWLETTVEDPGTGQARLAVERGADVVL
                     ACGGDGTVRSVAQALAGTGVAMGLVPAGTGNLLARTLGTPDNVAAAVRIALTGDDRKI
                     DVGRIRVDGSADEHVFLVMAGTGFDAAIMESTPEALKVRVGPLAYVISGLRAMRGRRT
                     RVTLTLDDGTPLRRRTRTVVVGNSGTLLGGLVLMPSATIDDGVLDVVNIAPKGLAGWV
                     AVAGRVISKRRAGNSRVEHWQARSILIEAEAPQPSQLDGDPIGDATRLQIRVEPAALV
                     MRVPDTPPPDVPDPG"
     gene            complement(7712..8830)
                     /gene="ald"
                     /locus_tag="VFE39_12175"
     CDS             complement(7712..8830)
                     /gene="ald"
                     /locus_tag="VFE39_12175"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET6259399.1"
                     /translation="MRIGVPREVKNHEYRVALTPAGAHELTRSGHHVLVEQGAGLGSS
                     ITDADYEAVGARIVATPDEVWSAADLLLKVKEPIEQEYRRLRRGQVLFTYLHLAASLP
                     CTEALLAAGTTAIAYETVRLPDGTLPLLAPMSEVAGRLAPQAGASALMRAAGGRGVLL
                     GGVPGVRPASVVVIGAGVSGQNAVAMAVGLGADVTVLDLDITKLRRLDDRYAGRVRTI
                     VSNAHELEKACLGADLVIGAVLVPGARAPRLVSNDLVAAMKQGSVLVDIAIDQGGCFE
                     DSRPTTHDDPTYRVHGSVFYCVANMPGAVPHTSTYALTNATLPYVLRLANLGWADALA
                     ADPALAAGLSTHDGALTSLEVAHDLGLPYAEPRGLVAA"
     gene            8898..9437
                     /locus_tag="VFE39_12180"
     CDS             8898..9437
                     /locus_tag="VFE39_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET6259400.1"
                     /translation="MPLPPAGALPARALPPQDVRPLDSTDHAILRVLGSDARISNKDL
                     AERVGVAQSTCLARVRTLHESGVIRGYHADIDPRALGHDLQAMIAIRLQPHARAVMGD
                     LVAELSRRSEVLDVYFVAGANDFLVHVATASTDELRRFVADHLNRDPAIAGTETNLIF
                     EHTRAAGRRGSVSAPDQPS"
     gene            complement(9445..10656)
                     /locus_tag="VFE39_12185"
     CDS             complement(9445..10656)
                     /locus_tag="VFE39_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HET6259401.1"
                     /translation="MRYDIQAIRDRFPALKAGYSHFDGPGGSQVPDVVADAVARTLVS
                     PLANRGRVTAAERTADDIVTGARQAMADLLGTDPGGIVFGRSMTQLTFDMARTLAATW
                     RPGDEVVVTRLDHDANIRPWVHAAEAAGAVVRWADFDPATGELTEEDVAAVLSPRTRL
                     VAVTAASNLIGTCPPVRAITDLAHEAGALAYVDGVHFTAHAAVHLEALGADFYACSPY
                     KFLGPHCGVLAAAPALLADLHPQKLLPSTDTVPERFELGTLPYELLAGTTAAVDFLAS
                     LGSSSVDPALGRREQLVCAMSVVGQHEDRLRVRIEEGLAALPGVTVYSRAASRTPTLL
                     VGFADREPGAAYEFLAGRGVNAPASSFYALEASRRLGLGDGGALRIGLAPYNDADDVD
                     RLLEGLRAFLA"
     gene            complement(10745..12142)
                     /locus_tag="VFE39_12190"
     CDS             complement(10745..12142)
                     /locus_tag="VFE39_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259402.1"
                     /translation="MVDRMFASEGDSVNVGRQGSTRWPDRWDDVPPQGPSGYEAAADV
                     ARRLRARLDRQLWLLDRMRTEQLYPEPAALPDLLEASRRMQRDTESLLLLCGQERATR
                     DTAPRRLSDLLADAASAAEEPHRVDVRHAPAATVSAGAATELLHVLAELVEDVTVVYP
                     GVHLDVASRIEARALVVDVSVDGGPHHDPDGFDGRGVAVVAEQLAQRSHHGISLHRPL
                     AGPPRTGSGPVASVHCPPNAITVDEPAPVLSDPLLTIGNGIGSLGNGSMQPSNGSAQL
                     SNGSGLLSNSSGLAGNGSGYFGNGSGHPDVPPPVEPQEPLYSPSAPSQVDELFGPLLD
                     LPLEPIDDRYATPIFEAIASAWFRDPEPAAALGDPGGQTNGADPLNWESPNDSEWQAA
                     AARAARSDPAPVTPSGLPRRRPGNQLVPPPRVQGTNPARERVPDRVRERLTTYQRGLR
                     QGRHRAPGSDEPDAW"
     gene            12530..13645
                     /locus_tag="VFE39_12195"
     CDS             12530..13645
                     /locus_tag="VFE39_12195"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HET6259403.1"
                     /translation="MTGVRVLVGTRKGAFVLTSDGKRERWDVSGPHFSGWEVYHVTGS
                     PADPDRVYAAPSGGWFGQLVQRSDDGGVTWSAVGNEFVYEGVPGTHQWYDGTPHPWEF
                     ARVWHLEPALNDPDTVYAGVEDAALFRSADGGRTWQELPGLREHGSGPQWQPGAGGMC
                     LHTVVLDPRDPARMHVAISAAGVFRTDDAGKTWQATNRGLQSEGIPDPDAEVGHCVHN
                     LAMHPSRPDVLFMQKHWDVMRSDDGGDTWHDIGGNLPSDFGFPIAVHAHEPETVYVVP
                     ITSDSEHFPPEGKLRVYRSRTGGNEWEALTNGLPQQHCYINVLRDAMAVDSLEDCGVY
                     FGTTGGQVYASSDAGNPWAPIVRDLPAVLSVEVQTIP"
     gene            13642..13965
                     /locus_tag="VFE39_12200"
     CDS             13642..13965
                     /locus_tag="VFE39_12200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6259404.1"
                     /translation="MSDPITATAPIRIKLPAHLRKLAQVDGEVTLDLAGPPTLRMVLD
                     TLERTYPVLRGTIRDHDTHRRRAFVRYFACEQDISHASPDDPLPDAVAAGSEPLLIVG
                     AMAGG"
     gene            complement(14009..14629)
                     /locus_tag="VFE39_12205"
     CDS             complement(14009..14629)
                     /locus_tag="VFE39_12205"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HET6259405.1"
                     /translation="MARYFDVHPIDPQRRAIGQVGDIVRSGGLIAYPTDSCFALGCQL
                     GDQEGLERIREIRHLDDKHHFTLVCRDFAQLGQFVQVSNTVFRTIKAATPGSYTFILP
                     ATKEVPRRLLHAKKKTVGVRIPQHTVAQALLAELGEPLLSSTLLLPDEPEPLTQGWEI
                     KERLDNALDAVLDSGDCGTEPTTVVDFSGPEPEIVRRGAGDPARFE"
     gene            14860..15258
                     /locus_tag="VFE39_12210"
     CDS             14860..15258
                     /locus_tag="VFE39_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259406.1"
                     /translation="MTSTRFTDFLTVPFATLSVVAFLRFREPGLHRAGEPAALRQHVA
                     ATFRILTQQQRLVPVIALAVLSSLLLQSIFEFGPLWLVAAMPVIMYGPYWAGLMATLG
                     LGGLLAGRLRLDRPAPWPPRSVSCCWPAWR"
     gene            15622..16062
                     /locus_tag="VFE39_12215"
     CDS             15622..16062
                     /locus_tag="VFE39_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HET6259407.1"
                     /translation="MLMRTDPFRELDRLTQQVLGTATGTCSRPTAMPMDAYRAGDEFV
                     VAFDLPGVAPDAIDLDVERNVLTVRAERRPTVSGEQVEMQVAERPLGMFSRQLFLGDS
                     LDTEHIQAAYEAGVLTLRIPVAEQAKPRKIAITGADEEPRQINA"
     gene            16717..17892
                     /locus_tag="VFE39_12220"
     CDS             16717..17892
                     /locus_tag="VFE39_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET6259408.1"
                     /translation="MLVAAVVLLVASGSGTAAPAGSTAAAAGATPPHVRLVPADGATA
                     VAPAAPIRVQATGGTLAGITLTADDGTSIPGTLSRDRRTWTASAAPAYGTIYRFGGRA
                     INDGGSVPVTGGFTAVDPTQLVRASTSIGDGAVVGVAAPIDIRFDRRIAEAERATIQR
                     GLKVRTTVPVEGSWAWLPDNDRGSRVHWRPATYWPSGTGVTVEDPLYGRDLGLAGFGA
                     ADLTSTFSIGRSQIVRADIGSHRMIVIRDGKEVTNVPATYGLESDPRRVTRGGTHVVM
                     SKSSTVLMSNPAFNYENIPMHWAVRISNNGEFIHANPASGYAHGRRNVTHGCVNLSTS
                     NARAYFDSALYGDPVEVTGSTIPLSSRDGDIYDWTIPWDQWKTMSALPSSVARRLPR"
     gene            18184..>18973
                     /locus_tag="VFE39_12225"
     CDS             18184..>18973
                     /locus_tag="VFE39_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259409.1"
                     /translation="MLRSQPVIGSIEQDRRGSEEPLPRRWTVFGVVSVALLMASIDQT
                     SVATALPAIQHDLGGSLAWTSWTITVYALGQIMAMPIAGKLSDQFGRKNIFLAAIVLF
                     TVTSLCAGMAGDIAILIIVRVFQGMAAGAIMPSATGIVADQFGRDRDRAIGLFTSIFP
                     IGAIIGPIVGGVLVTYTSWRAIFLINLPLGVLLLILVPVLIRRTPRTRAAHHIDLKGI
                     GLLCGLLLSTMTTITALGTPGDLRLQVTAAAVAGVVAAVLGWRFL"
BASE COUNT         2643 a         6953 c         6692 g         2685 t
ORIGIN      
        1 ctccgccgcg gcgggctccg tcgtccccgg aaccgtggtg ctctggggcc gtgtcgtggt
       61 catgaccgcc gtcctcccat gctcggaccg gccgccgcac ggatgcgcgc cacggcaccg
      121 gcgcggccgc cgcaggcacg accctagtcg atcccgtctg cagtggatcc tcgctgtcgg
      181 tcggggatcg gcgcgagcgc gcgcacagcg gacagtgcag actgcagtcg tgcccgttca
      241 gctcgagcgg cggaccaggc tgccgccgga cgcccgatcc gccggacgag cgcggcgcat
      301 gctccaggag gctctgcggc ccctcgacct gggtggagga gacggcgccg acggcgacct
      361 cgtcgacacc gtcgtgctgc tcgccagcga gctgtgcgag aacgccgtcc tgcacgcagg
      421 caccgagttc gaggtggccc tcaccgtcac cgatgacgag gtcgccgtcg ctgtgacgga
      481 ccggggtgca ggtcccctcg agctgcacct cgcccagcca cgacagcgct acggccgtgc
      541 ggccagccac ggacgcggac tcgccctcgt ccagcggctc gccaccacct ggggcacccg
      601 ccacgagtcc gacggccgac acgtcatctg gttctcggtc ggccgcggtc ctgctgtggc
      661 cgccccgccg accccgcccg ccccgcccga cacccggcgc gtgtggagca ccgccgagca
      721 ggctcgatgg ttgctgcacg tcccgtcggc gctgggcgac cggctcgaac cggccgagct
      781 ggtggccgag ctgatccgca ggctgcgcga gctcctcgac gccgactccg tggacgtgga
      841 ggtcgacgaa ggcgacggca acggccccca ggagatcgcc agggacggcg ccggtcgggc
      901 cgggggaacg gtcgtcgaga tccgcctgcc catgacggcc ccgctgcgcg gcgtcctgcg
      961 ggtggtgcac cgccctggcg cgcaggcgcc gggtgtcgag gccgaccgca gacgcgacct
     1021 caccgagctg atcgccagcc gggtcgccat ggcggtcgag tcgaagtggc tgcgtggggt
     1081 cgaccagcgc aggcgggcgt ggatgaccta cctcgccgag acgagcgagc tgctcggcca
     1141 gtccctcgac gtcgagctgg ccgtggcggt ggtcccgcag gtcgtcgtcc cccggctcgg
     1201 ccggtggtgc gcggtgcacc tcatggagcc ctccggacgg cttcggctgg cagcgctcac
     1261 ccacgccgac gagaccaagc tcaccgagct gcgcggggtg ctggatccgg accgcactcc
     1321 cggcctgccc tccgagctgc gaacccggtt cgccgagctg gtccgcgacg gcacggcccc
     1381 ggtgcgcttc gccgtgccca ccgacgggat cgcgttgccg ctgcgcgcgc gcggccgcac
     1441 catcggcacc ctcctcgtcg gcaggcccga gggccgtccg cacaccccgg aggacgtcgt
     1501 gctggcgggc gacatcgccc gccgggccgc gctgacgatc cacaacgccc agagcacggg
     1561 cgcccacgtc gcggtctcgc aggcgctgca gcaggcgctg ctcccccgcg cactgccgat
     1621 ggcgccgggc ctcgacttcg cggccgagta cctcccggcg agcaccggca gcgacgtcgg
     1681 tggtgacttc tacgacgtgc tcaccgtcga cacgtcgcgc tggctggtat cgatcggcga
     1741 cgtctgcggc aagggcgccc gtgccgcggc ccgcaccggt ctcgtccgtg acgtgctgcg
     1801 ggtgctcatc cgggacggca ggccgctgcc gcgcgccatc gagatgctca accaggtgat
     1861 gatcgaagcg ggcgacccgc tgcagttctg caccatcgcc gcggccatgg tcagcaggcg
     1921 tcgaccggtg gccatcgacc cctcgccccg caccggacac accggcctcg acgtcgagct
     1981 cgtgctggcc gggcatgtcc agccggtgct cgtgcgggcc gatggcgccg ccgagctcat
     2041 cgggcagttc ggcacggccg tcgggctggt ccccagcatc cgcctggagt gcacgccgca
     2101 ccggctggcg cccggcgaca cgcttctcgt ctacaccgac ggggtcaccg aacgccgccg
     2161 cggccgcgag cagttcggct ccgagcggct gctcgacgtc gctacgaagg cggctggacg
     2221 accggcgaga caggtggtcg cggccgtacg ggaggcggtc gagcggttct ccgccgaccc
     2281 gctcaacgat gacgttgcac tactggcggt acgcgcgacc ccctgaccag gccggatcgc
     2341 cggcgtcgcc ccatggccgc cagattggcg gctgtccggg taccgctgga gcccgctgtc
     2401 acgcatcctg gtgcttcaac ggcggcggcg atctcgcccc atggaggagg agcgctggac
     2461 aagaagccca gcgggcggac ggtgctccgc gaactccgtt cgtggccgac gctcctgctc
     2521 gtgctgttct gcatcggcgt cggcgtcccc gtcacggtca cgctcacccc ggcccaggac
     2581 ctcgtcgcct tcggccagca catctccgtc ggcgcccggc caccggcgct gtccgtcgcc
     2641 ggcccggcac gactcgtgca ggtcgggaac acggccctcg acgtcgaccg cctgcacgtc
     2701 tacggaccgc tgcgcccgca gctgaccatg ggcccggtgc agcgcagcgc ggcggcagcc
     2761 gccgtgttcg acccggtcga gggcccagag gtccgcgatg aggccgtgtc gtcggtcacc
     2821 accgcgttca tcacctggta cgtcctcggc gggctcggcc tgatcgccgt cacgttggcg
     2881 gccgccgcgg gcgcggcggg tctgcgcacc ctcgtcgtac tgcgccgcca gagccgcgag
     2941 accggtggac acgcaccgct gcacgagatc ttcacctact gcgtgcgggc ggcccgccgg
     3001 atgaccgcga tcgcgctcgt cgcgtccgtc gtggcatggg gtgtctcggg ggtgctcgcc
     3061 tacaccggcg ccgtgcaggg gctgcgcggc gtgtcgtcgc tcgcccagct cgtcggcgcg
     3121 taccacctga gccccgatcc ggtcgggccg cccctcagcg gctacgacgg cgcggtgatc
     3181 ggggactcgc gggccgtacg cgtcggcggt cccccgctgc ccggcggcac acccgacgac
     3241 gccgcctgcg agcgcagcac cgactcgctg gccgccgaga tcggtcacct ggcctcggca
     3301 aaggtgctca acctcgcctg tccgagcgcg accgtcacga cagggctgcg tggcccgcag
     3361 cagcgcggcg accgagtggt tcccccgcag atcggcgtgc tcaaacaggt gcaggacctc
     3421 cgcttcgtcg tcgtggcgat cggcccgaac gacgtcggct ggaccgactt cctgcgctac
     3481 tgctacggcg tggccgactg ctccgaccag ctcacccagg gcgagttcga ctaccgcatg
     3541 gcggcgttcg accgcgtcta cggcgacctg ctcgtcgacc tcaacgacct gccgggccgc
     3601 ccccaggtca tcgtgatgac gtcctacggg gcgttccgtc ccgacgccga ctgcgccgac
     3661 acccgggccg cgggccaccc ggggctggac ccggcgaaga tcgagctgct gaccgcgcgc
     3721 aacgagcagc tcaacgcggt gctgagcgcc ggcgcggaga agtacggctt cgcggtggcc
     3781 cgccccgagc tggcgctgct gtgcgaggac ggcaccgacg ggctgggccc ggacctgcag
     3841 gggatggccg acccgttccc cttccacccc acggggatcg ggtcgctccg gatggcgtcg
     3901 tcggtgctgc ggctgatagc gcccgcccag gagcgctgac cgcgccccgg catgccctct
     3961 ctcccggtac agcgcaggtg ctgtgtcggt cccggcgggc acactgtccg cgtggggtac
     4021 tgggtactgc acgtcgacct cgaccagttc atcgccgcgg tcgagatcct gcgccgaccc
     4081 gagctggcag gcgcgccggt cgtcgtcggc ggcgcgggcg acccgacgca gcgcgcggtc
     4141 gtcgccaccg cgtcctaccc tgcgcgcgag cacggtgtgc gttccgggat gccgctgcgc
     4201 acggcggcca ggcggtgccc cgacgcggtg ttcctgcccg cggacaacgc ccactacgag
     4261 gaggtctccg aggaggtcat ggccaccctg cgccggattc ccgatgtggt ggtcgaggtg
     4321 atgggctggg acgaggcgtt cctcggcgtg gagaccgacg accccgagtc cctggcccgg
     4381 gaggtacggg ccgccgtgct cgcggcgacc cgcctgtcgt gcacggtagg catcggcgac
     4441 aacctgctgc gcgcgaagct ggccaccgag ttcggcaagg cgtcgcccgg gagcgaacgc
     4501 gggcccggca tgggtacggg cgtcttctgc ctcaccgctg ccaactggga ggaggtgatg
     4561 gccgagcgtc cgacccaggc cctgtggggt atcggcacga agaccgcccg caagctcgcc
     4621 gagcggggcc tcaccaccgt gcgcgagctg gccgccgccg acccgggtgt actggccgcc
     4681 gacctgggcc cttcaatggg cccctggtac gtccagctcg ggcggggcat cggccgcacc
     4741 agggtggagg gcacgccgtg ggtgcgccgc tcgcgcagcc gcgagacgac cttccaggag
     4801 aacatcaccg tctgggacga cgtgcgcacc gaggtggccg cgctggcccg ccgcgtcgcc
     4861 cgggacatca cggtcgatgg caggccggcc gcacgggtgg ctgtcaaggt gcgcttcgcg
     4921 cccttttcca cctacacccg cagcgcgacg ctcgacgcgc cgacctatga cggcgaccgg
     4981 atcgaggcgg gtgcgctgcg cgtgctggag ctgttcacct cgcgacgccc ggtgcgcctg
     5041 ctcggggtgc gggcggagtt cgagcagccg tagcgaccct gctgatcagc cgatctgcgc
     5101 ggggtcacgg tgcgccgcgc tcccgggcct cctccagcca ccgcaccgct tcctcgaccg
     5161 tccccaccgc gggcacgtcg ggtgcgggcg gccggtcgag cagcacgacg ggcacgccca
     5221 gctcgcgcgc ggcgaccagc ttggccgcgg tcatctcgcc cccgctgtcg cgggtgacca
     5281 gcacgtcgat gcgatgctcg cgcaacagct cgcgctcggc gtccgcggtg aacggcccgc
     5341 gggcgcgcac gacctcgcac cgggccggca ggggcggctg cggcgggtcg acggagcgga
     5401 gcaggaacca gagcccgtcg agcgccgcga acgcggcgag atccccgcgt ccggtggaca
     5461 ggaagacgcg ttcccccagg gtgggcagca gcgcggcggc gctcgccgcg tccgggaccc
     5521 ggtgccagcg gtctcccggg cctgccgtcc aaccgcggcg ccgcaggacg agcagcggca
     5581 cccccgcaac ggcggtggcc gcgacggcgt gcaccgtcat ccggacggcg aacggatgcg
     5641 tggcatccac caccacgtcg gtcccgtgcc ggcgcagcca cgcggccagc ccgtcgacgc
     5701 cgccgaaccc gccgatccgg acgtcccccg gcgggagtga gggcaccgcc acccgcccgg
     5761 ccaacgagga cgtgacgacg aacttccgtt cgttcagtgc gtcggcgagc cgccgggcct
     5821 gcccggtgcc gccgaggacg aggaccttca cccgtcccgc cgccgcagca ggtaggtgtc
     5881 catgatccag cccttgcgct cgcgctgctc ggccctgacc cgcacgatct cgcccttgac
     5941 ctgctgcagg tccccgtgca ggagcacctc gtccgccgtg cccaagtagg caccccagta
     6001 gatgtcgagg tcggtgtcgg gaagcgtggc aaaagcgttg tgtgcgtcga gcatgacgac
     6061 gacgtcgtca atgcccgccg ggagaccctg ggctgcgagc ctgcgtcctg tcgtgaccat
     6121 gaacgggcgc ccgacccggt tgaggatcag ccggtgccgc gccgcgagcg cctgcacgct
     6181 gctgatcccc gggatcgact cgacctcgaa ccccacggca ccgcgcgcgc cgatgcgctc
     6241 caggatgcgg agcgtgccgt cgtagagggc ggggtcgccc cagacgagga aggcgccgca
     6301 tccgtcctcg tcgagctcgt cggcaatcat ccgctggtag atcgactcgc gacgctcctg
     6361 ccagtcgacg acggcggcct cgtacccgcc cggcgccgcg gcccggtccc gctccgggtc
     6421 gccggcctcc accacgcggt ggtgtcgcgt cacgtgccgg tccaggagct cgatacgcag
     6481 ccgcgccagg tcgcccttgt cgtcgccctt gtcgatggtg aagaagacgt cgacacggtt
     6541 catcgcctcc accgcctgga gcgtgaggtg ctgggggtcg cccgcgccga taccgatgac
     6601 gtagatcctg cgcactgctg gacgctaacc cggatccggc acgtcgggcg gaggggtgtc
     6661 gggcacgcgc atgacgagcg cggcgggctc cacccggatc tgcagccgtg tcgcgtcccc
     6721 gatcgggtcg ccgtcgagct gggatggctg cggcgcctct gcctcgatga ggatcgaccg
     6781 cgcctgccag tgctcgaccc gggagttccc tgcgcgccgc ttgctgatca cgcgaccggc
     6841 caccgcgacc cacccggcga ggcccttcgg ggcgatgttc acgacgtcga gcacgccatc
     6901 gtcgatcgtg gcactcggca tcaggacgag cccgccgagc agcgtgccgc tgttgcccac
     6961 gacgaccgtg cgggtgcggc ggcgcagcgg agtcccgtcg tcgagggtca gggtgacgcg
     7021 cgtgcgcctg ccccgcatcg cccgcagccc ggagatcacg taggcgagcg gaccgacccg
     7081 caccttcagc gcctccgggg tgctctccat gatcgcggcg tcgaacccgg tgcccgccat
     7141 caccaggaag acgtgctcgt ccgccgagcc gtccacccgg atccggccga cgtcgatctt
     7201 ccggtcgtcg ccggtcaggg cgatgcggac agccgcggca acgttgtcgg gggtaccgag
     7261 ggtgcgggcc agcaggttgc cggtaccggc cggcacgaga cccatcgcca ccccggtacc
     7321 ggcgagcgcc tgcgcgacgg accgcaccgt gccgtcgccg ccgcaggcga ggacgacgtc
     7381 ggcgccgcgc tcgacggcga gccgggcctg gccggtgccg ggatcctcga cggtggtctc
     7441 cagccacagc ggctccgccc agcccaggcc cgcgcagacg gactcgatcc gcctccgtgt
     7501 gccaggctcg atcttggtcg ggttcgcgac gaccgcgggc aatgggcggt cctccggctc
     7561 cgcggtcaac gagtccggat cggtgcccga cgggcggctc ctgaccgcca cggccacgag
     7621 cagagccagc acgggcaggg cggccagcag gaggatccac cagtcgccgg acacggcgga
     7681 acaataatcg tccagccgcg ggcaacgggc ctcaggccgc gacgagcccc cgcggctcgg
     7741 cgtagggcag gccgagatcg tgggccacct cgaggctggt gagggcgccg tcgtgggtgg
     7801 acagacctgc ggccagcgcg ggatcggccg cgagcgcgtc agcccaaccg aggttcgcca
     7861 ggcgcagaac gtagggcagg gtggcgttgg tcaacgcgta ggtcgaggtg tgcggcaccg
     7921 cgccgggcat gttcgccacg cagtagaaca ccgagccgtg cacgcggtag gtcgggtcgt
     7981 cgtgtgttgt gggccgggag tcctcgaagc agccgccctg gtcgatggcg atgtcgacga
     8041 gcacggagcc ctgcttcatc gcggcgacga gatcgttgct caccagccgg ggcgcccggg
     8101 ctcccggcac caggacggca ccgatcacga ggtcggcacc gaggcaggcc ttctcgagct
     8161 cgtgcgcgtt ggacacgatg gtgcgcacgc ggccggcgta gcggtcgtcg agtctgcgga
     8221 gcttcgtgat gtcgaggtcg agcacggtga cgtcggcgcc gagcccgacg gccatcgcca
     8281 ccgcgttctg cccggagaca cccgcaccga tcaccacgac gctcgccggg cgcacgccgg
     8341 gcacgccgcc gagcagcaca ccacgcccac cggcggcgcg catcagcgcg ctcgccccgg
     8401 cctgcggcgc gagccggccc gccacctcgc tcatcggagc gagcaacggc agcgtgccgt
     8461 caggcagtcg gacggtctca taggcgatcg cagtggttcc ggccgccagc agcgcctcgg
     8521 tgcacggaag cgacgcggcg aggtggaggt aggtgaacag cacctggccg cgtcgcagcc
     8581 ggcggtactc ctgctcaatc ggctccttca ccttgagcag caggtcggcg gcgctccaga
     8641 cctcgtcggg cgtggcgacg atgcgagcgc ccacggcctc gtagtcggcg tcggtgatcg
     8701 agctgccgag gcccgcgccc tgctccacga gcacgtggtg cccggagcgg gtcagctcgt
     8761 gcgccccggc aggggtcagc gcgacacggt actcgtggtt cttgacctcg cggggcactc
     8821 cgattcgcat aagcgcagaa tgaagaaatc actgaacacg tgcaatagtt gcgcagaaga
     8881 ttcggtatcc tgcttctatg ccactgcctc ctgcgggcgc actgcccgcc cgagccctgc
     8941 cgccgcagga tgttcggccc ctcgacagca ctgaccacgc gatcctccgt gtcctcggca
     9001 gcgacgccag gatctccaac aaggacttgg ccgagcgggt cggcgtcgcc cagtcgacgt
     9061 gcctcgcgcg ggtgcgcacg ctgcatgaga gcggcgtgat ccggggctac cacgccgaca
     9121 tcgaccccag ggcactcggc cacgaccttc aggcgatgat cgcgatccgg ctgcagccgc
     9181 acgcgcgggc cgtgatgggc gacctggtgg ccgagctcag caggcgcagt gaggtactcg
     9241 acgtctactt cgtcgccggg gcgaacgact tcctcgtgca cgttgccacg gcgagcaccg
     9301 acgagctgcg ccggttcgtc gccgaccacc tcaaccgcga tcccgccatc gccggcaccg
     9361 agaccaacct gatcttcgag cacacgcgcg cggccggcag gcgcggctcc gtctccgcgc
     9421 ccgaccagcc atcgtgaccc cggctcaggc gaggaacgcg cggagcccct cgaggagccg
     9481 gtcgacgtcg tcggcgtcgt tgtagggcgc gagcccgatg cgcaatgcac cgccgtcacc
     9541 gagaccgagg cgccgcgacg cctcgagggc gtagaaggag ctcgcgggcg cattcacgcc
     9601 gcgcccggcg aggaactcgt acgccgcgcc gggctcccgg tcggcgaacc cgaccagcaa
     9661 cgtcggcgtc cgagaagccg cgcgggagta cacggtcacc ccggggagcg cggcgagccc
     9721 ctcctcgatg cggacccgca gccggtcctc gtgctgcccc accacgctca tggcgcagac
     9781 cagctgctcc cgcctaccca gcgccgggtc gacgctcgac gaacccagag acgccaggaa
     9841 gtcgacggcc gcggtggtgc cggcgagcag ctcgtagggc agggtgccga gctcgaaccg
     9901 ctccgggacg gtgtcggtgg aaggcagcag cttctgcggg tgcaggtcgg cgaggagcgc
     9961 aggcgccgcg gcgagcacac cgcagtgcgg gccgaggaac ttgtacgggg agcaggcgta
    10021 gaagtccgcg cccagcgcct cgaggtgtac ggcggcatgc gccgtgaagt gcaccccgtc
    10081 cacgtaggcg agggcgcccg cctcgtgcgc gagatcggtg atggcccgca cgggcggaca
    10141 ggtgccgatc aggttcgacg ccgcggtgac ggcgacgagg cgcgtgcgcg gtgagagcac
    10201 ggcggcgacg tcctcctcgg tgagctcacc cgtcgcgggg tcgaagtccg cccaccggac
    10261 gaccgctccc gctgcctccg ccgcgtgcac ccacggccgg atgttggcgt cgtggtcgag
    10321 ccgggtgacc acgacctcgt cgccaggtcg ccaggtggcg gccagggttc gggccatgtc
    10381 gaaggtcagc tgggtcatgc tgcggccgaa cacgatcccg cccgggtcgg tgccgagcag
    10441 gtccgccatc gcctgccggg ctccggtcac gatgtcgtcg gcggtgcgct cggctgccgt
    10501 gacccgaccg cggttggcca gcggggagac cagtgtgcgg gccaccgcgt cagcgacgac
    10561 gtccggaacc tgcgaccctc ctgggccgtc gaagtgggag tacccggcct tcagcgcggg
    10621 gaagcggtcg cggatggcct ggatgtcata gcgcatacgc gaaccttgtc atgagcatcc
    10681 gggagcggca tcccacgtgt gatcatccca cctgtgatct gacccggggc cgaggacacc
    10741 ggcgtcacca ggcgtcgggc tcgtccgagc cgggcgcgcg atggcggccc tggcgcaacc
    10801 ctcgctggta ggtggtgagg cgctcccgca cccggtcggg gacgcgttct ctggcggggt
    10861 tcgtgccctg gacgcgcggc ggcggaacga gctggttgcc gggacggcga cgcggtagac
    10921 cgctcggcgt gacgggcgcg gggtcggagc gagcggcccg cgcggcggcc gcctgccact
    10981 cgctgtcgtt cggcgactcc cagttcagcg ggtcggcgcc gttggtctgg ccgcccgggt
    11041 cccccagggc cgcggcgggc tcggggtcac ggaaccatgc cgatgcgatg gcctcgaaga
    11101 tcggggtggc gtaccggtcg tcgatcggct cgagcggcag gtccaacagc gggccgaaca
    11161 gctcgtccac ctgagagggc gccgacgggg agtacagcgg ctcctgcggc tcgaccggcg
    11221 gcgggacgtc ggggtgtccg gagccgttgc cgaagtaacc agatccgttg cccgcgagac
    11281 cggaactgtt gctcagcagc ccggaaccgt tgctcagctg cgcggatccg ttgctcggct
    11341 gcatggatcc gttgcccagc gagccgatgc cgtttccgat cgtcaggagc ggatcggaga
    11401 gcaccggagc gggctcgtcg acggtgatgg cgttaggcgg gcagtgcacg ctcgcgaccg
    11461 gcccggagcc ggtgcgagga ggcccggcga gcgggcgatg cagggagatg ccgtggtgcg
    11521 aacgctgggc gagctgctcg gcgacgacgg ccacgcctcg cccgtcgaag ccgtcggggt
    11581 cgtggtgcgg cccgccgtcc accgatacat cgacgacgag ggcgcgcgcc tcgatacgac
    11641 tcgcgacgtc gagatgcact cccgggtaga ccacggtgac gtcctcgacg agctcggcca
    11701 gcacgtgcag caactccgtg gccgcgccgg ccgagacggt ggcggcggga gcgtgccgga
    11761 cgtcgacgcg gtgtggctcc tcggcggcgg acgcggcgtc ggccagcagg tcggacagcc
    11821 gccggggggc ggtgtcacgg gtcgcgcgtt cctggccgca cagcagcagc aagctctcgg
    11881 tgtcgcgctg catccggcgg ctggcctcga gcaggtcggg aagcgcggcg ggctcggggt
    11941 agagctgctc cgtccgcatc cggtccagca gccacagctg ccggtcgagg cgagcccgca
    12001 accggcgcgc cacgtcggcc gcggcctcgt agcccgacgg gccctgcgga gggacatcgt
    12061 cccaccggtc gggccaccgt gtggatccct ggcgtccgac gttcaccgag tcgccctcgg
    12121 atgcgaacat cctgtcgacc accagtcgat cctctcgaac gtgttcgatg cgccgctccc
    12181 ctcctcgggg gccgtgcgtg ggtgcggcac agccgggcaa cgcggagtgg catctcctgg
    12241 cgtagcacgt tacatcgcga tgctccacct gcgggacagg tcacgggggc tgcgctcagg
    12301 acacgccccc atgcggcggg cgggcacgga cagtacggcc gacaatgaac cggcagcaca
    12361 gtagcccaca gcggacgagc gggcgacggc cggggggacg agcggccgcg atcctgcgat
    12421 caaccagatg ggcggacttc gggacgcacc gatgagttcc cgcctcggcg ccggtcacca
    12481 ttgccgtgcg ccgtgcggtg cggcgcgaaa tgtggagtgg aggcctgcgg tgacgggtgt
    12541 tcgggtactg gtcggcacgc gcaagggtgc gttcgttctg acctccgacg gcaagcgcga
    12601 gcgctgggac gtcagcggcc cgcacttctc cgggtgggag gtctaccacg tgacggggtc
    12661 acccgccgac cccgatcgcg tctacgccgc gccgtccgga ggctggttcg gtcagctcgt
    12721 gcagcggtcc gacgacggcg gcgtcacgtg gtcggcggtg ggcaacgagt tcgtctacga
    12781 gggcgtgccc ggcacccacc agtggtacga cggcacaccg cacccctggg agttcgcgcg
    12841 ggtctggcac ctggagccgg cgctgaacga tccggacacg gtctacgccg gcgtcgagga
    12901 cgccgctctg ttccgctccg ccgacggtgg ccgcacctgg caggagttgc ccggactgcg
    12961 cgagcacggc tcgggcccgc agtggcagcc gggagcgggc ggcatgtgcc tgcacacggt
    13021 cgtgctggat ccgcgtgatc ccgcccggat gcatgtcgcg atctcggcgg caggcgtgtt
    13081 ccgcacggac gacgccggca agacctggca ggcgaccaac cgggggctgc agtcggaggg
    13141 catccccgac cccgatgccg aggtggggca ctgcgtgcac aacctggcga tgcacccgtc
    13201 gcggccggac gtgctgttca tgcagaagca ctgggacgtc atgcgcagcg acgacggcgg
    13261 cgacacctgg cacgacatcg gcggcaacct gcccagcgac ttcggcttcc cgatcgcggt
    13321 gcacgcgcac gagccggaga cggtgtacgt cgtgccgatc accagcgact cggagcactt
    13381 cccgccggag ggcaagctgc gggtgtaccg cagccgcacc ggcgggaacg agtgggaggc
    13441 cctcaccaac ggcctgccgc agcagcactg ctatatcaac gtgctgcgcg acgcgatggc
    13501 cgtcgactcc ctcgaggact gcggcgtgta cttcggcacc accggcggcc aggtgtacgc
    13561 gtcatcggac gccggaaacc cgtgggcgcc catcgtgcgg gacctgcctg cggtgctgtc
    13621 ggtggaggtg cagacgatcc catgagcgac ccgatcaccg ccaccgcgcc gatccggatc
    13681 aagctgcccg cccacctgcg gaagctcgcg caggtcgacg gcgaggtcac gctcgacctc
    13741 gcgggcccgc ccactctgcg catggtgctc gacaccctgg agcgcacgta tccggtgctg
    13801 cgcggcacga tccgcgacca cgacacgcac cggcgtcggg cgttcgtgcg gtacttcgcc
    13861 tgcgagcagg acatctccca cgcctcgccc gacgacccgt tgcccgacgc ggtggcggcg
    13921 gggagcgaac cgctcctcat cgtgggggcg atggccggtg gctgagcagg ccgtcctcga
    13981 cggtgcggcg cccgaccggt aggccggatc actcgaaccg ggcagggtcc cccgcgcccc
    14041 ggcgcacgat ctccggttcg ggcccggaga agtcgaccac ggtcgtgggc tcggtgccgc
    14101 agtcgccgga gtcgagaacg gcgtccagcg cgttgtcgag ccgctccttg atctcccagc
    14161 cctgggtgag cggttcgggc tcgtcgggca gcagcagtgt ggacgacagc aggggctccc
    14221 ccagctcggc gagcaacgcc tgggccacgg tgtgctgggg gatccgcact ccgactgtct
    14281 tcttcttcgc gtgcagcagc ctgcggggca cctccttcgt ggcaggcagg atgaacgtgt
    14341 agctgcccgg cgtcgccgcc ttgatcgtgc ggaagaccgt gttgctgacc tgcacgaact
    14401 gcccgagctg ggcgaagtcg cggcagacga gcgtgaagtg gtgcttgtcg tcgaggtggc
    14461 ggatctcccg gattcgttcc agcccctcct ggtcgcccag ctggcacccc agcgcgaagc
    14521 aggagtcggt cgggtaggcg atgagacccc ccgatcggac gatgtcgccg acctggccga
    14581 tcgcgcgccg ctggggatcg atcgggtgca catcgaagta ccgcgccatg cggagagctt
    14641 agggcgggag cagcacggac gggtgtaacg ccggggcgcg gtcgcggcac tgcacagcca
    14701 tcgatcccct caaccgatca gaggccgtga tgctgatgcg cacgccgggc ccgcaccggg
    14761 ccgaaccgcg gctctccacg atgcgccggc gcctgctgcc cctgcacgtc gagcgccgct
    14821 ctcgtcgcct gcgcgttggc gggtggctgg atcgccggca tgacttccac ccggttcacc
    14881 gacttcctga ccgttccgtt cgccacactc tcggtcgtcg ccttcctgcg gttccgggag
    14941 cccgggctgc acagggccgg ggagccggcc gcgttgcggc agcacgtcgc cgccacgttc
    15001 cgcatcctca cgcagcagca gcggctggtg cccgtcatcg cgctcgccgt gctgtcctcg
    15061 ctcctcctgc agtcgatctt cgagttcggc ccgctgtggc tggtcgccgc catgccggtg
    15121 atcatgtacg gcccctactg ggccggcctg atggccacgc tcgggctcgg cgggctgctc
    15181 gccggacggc tccggctcga ccggcccgcc ccctggccac cgcggtcggt ctcatgctgc
    15241 tggccggcct ggcgctgacc accgccacca gctccgtcgt cgtgatgatc gcccagatcg
    15301 tgctggcgct gctcctggtg gccgccggca tccacgtcac ccacctcctg cacgacgccg
    15361 tgccctcgac ggtgcgcgcc ggcgtcgcct ccggcgtcgg cgcgatgtcc tggatcgtgt
    15421 tcctgccctt cgccctgaca gcagcggcgg cctcagccgc cgtcatcggg aatctcaccc
    15481 gaccgaggta tgaccgccag cttgcactcc acaaaacctc tatctacgct tacaggtatc
    15541 tggagtggct cccggccatc cccgggtcgc caccccggtg catcgccgct ttgtgtcgat
    15601 cacgagagga gtggaccagc gatgctcatg cgcaccgacc ccttccgcga gttggaccgg
    15661 ctcacccagc aggttctcgg caccgccacc ggcacctgta gtcgtccgac cgccatgccg
    15721 atggacgcct accgcgccgg cgacgagttc gtggtcgcct tcgacctgcc cggtgtcgcc
    15781 cccgacgcca tcgacctgga cgtcgagcgc aacgtgctca ccgtccgcgc cgagcggcgg
    15841 cccaccgtgt ccggcgagca ggtcgagatg caggtggccg aacgccccct tgggatgttc
    15901 tcccgccagc tgttcctcgg cgacagcctc gacacggagc acatccaggc cgcctacgag
    15961 gccggtgtac tgaccctccg catccccgtg gccgaacagg ccaagccgcg caagattgcg
    16021 atcaccggcg ccgacgagga gccgcggcag atcaacgcct gacggcaggg cagatcaggt
    16081 cccttgacgg gagggggtga tcgatcgaca tccgcgagac gtcccagacg atcggtgccc
    16141 cgccggttta ccggcggggc accccgacat cctggccggc ctgtatgagc gggcgacccg
    16201 gcgggccggt gctggcgcgc agcacgagcg tggtcgacag ctccacccgc ggcatcgacc
    16261 ggctacgccc ggcgctagcc gcagcggcaa ccggacggcc tccgcccggc ggccggaccc
    16321 aggatgcccg cggccgccgg cgtgatcacc gcgaggcgca cgctatcgcc ccgtcacggc
    16381 gctgggaggc ggttcgcgcc catcacccgg cccaggccgt ggctacgctc cgcgagacgc
    16441 tttaccccgc acgagcctga gcgactccac cgttctcgcc acaatggcgt aatgggtcga
    16501 caatttcgat gctgcagtca ttctccgtta cgacacgggg tcgatgttca ccattctggg
    16561 taatggttgc ccgatcgaag gccgtaaggc cgaaatgaga tcccgacgtt attgacctgg
    16621 acccacacaa ttgttctcat aattccgggg aatcgactgc ggggagtaac ggtggtcagg
    16681 cccggaacgg atcgtgcgaa acggcgcgca gcagggatgc tcgtggcggc agtcgtgctg
    16741 ctcgtcgcgt ccgggtcggg aacagctgct cctgccggct cgaccgctgc cgccgcgggg
    16801 gcgacacccc cgcacgtccg actcgtcccg gccgacggcg ccaccgccgt ggcacccgcg
    16861 gcaccgatcc gcgtgcaagc gaccggcggc accctcgcgg gtatcaccct caccgccgac
    16921 gacggcacct cgataccggg cacgctgtcg cgggaccgcc gaacctggac cgcttctgcc
    16981 gcccccgcct acggcaccat ctaccgcttc gggggtaggg ccatcaacga cggcgggtcc
    17041 gtcccggtca ccggcggttt caccgctgtc gacccgactc agctggtccg tgcctcgacg
    17101 agcatcggtg acggcgcggt cgtcggggtc gccgccccga tcgacatccg cttcgaccga
    17161 cgcatcgccg aggccgaacg cgccaccatc caacggggtc tgaaggtgcg aacgaccgtg
    17221 ccggtcgagg ggtcctgggc gtggctgccc gacaacgacc gcggctcgcg agtccactgg
    17281 aggccggcca cctactggcc ctccggaacg ggggtcacgg tcgaggaccc tctctacggg
    17341 cgcgacctcg gccttgccgg gttcggcgct gccgatctga ccagcacctt cagcatcggc
    17401 cgctcccaga tcgtccgcgc cgacatcggg tcacaccgga tgatcgtcat ccgcgacggc
    17461 aaggaggtaa ccaacgtgcc tgcgacctac gggctggaat ccgacccgcg gcgggtcact
    17521 cgaggtggca cccatgtcgt gatgagcaag tcctccaccg ttctgatgtc caatccggcg
    17581 ttcaactacg agaacattcc gatgcactgg gccgtgcgga tctccaacaa cggcgagttc
    17641 atccacgcca accccgcttc gggttacgcg catggcagac ggaacgtcac ccacggctgc
    17701 gtgaacctgt ccacgtcgaa cgcgcgcgcc tacttcgact cggctcttta cggggatccc
    17761 gtcgaggtca ccggaagcac catcccgctc tcctcccggg acggtgacat ctacgactgg
    17821 acgattccct gggaccagtg gaagaccatg tcggccctcc cgtcatccgt ggcgcgtcgg
    17881 cttcctcggt aaggccccgg tcggacccac gccccctcct gcgactgcgc cgccggcacc
    17941 tgcacacggg gtgcccggga tcgcgggcgt cccggagggg cggatcgtct cgcgcaagct
    18001 cctcgcgatc gccgggctcg gcgaacacga gcaggacttg cccgacgccc tggcgttggc
    18061 ggcgccgtgg tgcccgaccg catcttcgac ctgcaccgcg tggtcccgca ccggccgccg
    18121 cggagcgggt gaaccttgtc cagccttttg cttgcttagc gtcaaacaat taatatccgg
    18181 tccatgcttc gctcccaacc cgtcatcggc tcgatcgaac aggatcgacg ggggagcgag
    18241 gagcccctcc cccgtcgctg gaccgtcttc ggcgttgtca gcgtcgccct gctcatggcc
    18301 tcgatcgacc agacgtccgt ggccaccgca ctccccgcga tccagcacga cctcggcggc
    18361 tccctcgcct ggaccagctg gacgatcacc gtctacgcgc tcggccagat catggccatg
    18421 ccgatcgccg gcaagctcag tgaccagttc ggacgcaaga acatcttcct ggccgccatc
    18481 gtcctgttca ccgtgacctc gctctgcgcg gggatggcgg gcgacatcgc catcctcatc
    18541 atcgtgcggg tgttccaggg catggcggcc ggcgcgatca tgccgtcggc caccgggatc
    18601 gtggccgacc agttcggccg ggaccgcgac cgtgcgatcg gcctgttcac cagcatcttc
    18661 ccgatcggcg cgatcatcgg gccgatcgtc ggcggcgtgc tcgtgaccta cacctcctgg
    18721 cgggccatct tcctgatcaa cctcccgttg ggcgtgctgc tgctgatcct ggtccccgtc
    18781 ctgatccgga ggacgccccg cacccgggcc gcccaccaca tcgacctcaa ggggatcggg
    18841 ctgctctgtg ggctgttgct gtccaccatg accacgatca ccgcgctcgg cacccccggc
    18901 gacctccggc tgcaggtcac cgcggcggcg gttgccgggg tggtggcggc cgtgctcggg
    18961 tggcgcttcc tgg
//
LOCUS       DASRSC010000249         5886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4026697,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000249 DASRSC010000000
VERSION     DASRSC010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4026697"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VFE39_12230"
     CDS             complement(<1..1164)
                     /locus_tag="VFE39_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6259410.1"
                     /translation="MPAEKSMDFGPSARRLIGRLAPERAGAVAVLVLGIISVTLSVIG
                     PKILGHATDVIFAGVIGRQIPPGTSTAEAAAAARAAGDETFANVIQGSGVVPGQGIDF
                     ALLSQVLLGVIAIYVAASLFGWLQGYMLNGIVQRTIFGLRREVEEKLNRLPLRYFDRQ
                     QRGEVLSRVTNDIDNISQTLQQTMSQLLTSLLTVVGVLLMMVLISPLLALVALVTIPV
                     SVLVTRAVAKRSQKQFVAQWRHTGALNAHIEEAFTGHELVRVFGRRREVEADFGEKNE
                     RLFDASFRAQFISGIIMPSMMFIGNLNYVLVAVVGGLRVASGSMSLGDVQAFIQYSRQ
                     FTQPITQLASMANVLQSGIASAERVFELLDAEEQVPDPAVPAVPSTRRGRVVFE"
     gene            complement(1284..3032)
                     /locus_tag="VFE39_12235"
     CDS             complement(1284..3032)
                     /locus_tag="VFE39_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6259411.1"
                     /translation="MLIRLLRERLRPYTRPLLLVVMLQLVGTMASLYLPSLNAEIIDQ
                     GIAKGDTDYIVGTGGWMLLVALVQIACSIAAVYVGARTAMGFGRDVRAALFHRVGEFS
                     AREVNRFGAPSLITRGTNDVQQVQMLVLLSCTMLVTAPIMCFGGVVMALQEDAQLSRL
                     MLICVPVLVLAIGAVIVRMVPGFRSMQVRIDEVNRVLREQITGIRVVRAFVREPEETA
                     RFARANADLTDVATRVGRLMAMIFPIVMLVLNVSSVAVLWFGASRIDAGQMQIGALTA
                     FLSYLIQILMAVMMATFMVMMIPRAAVCAERVIEVLDTESSVSLPERPVVPRAATGVL
                     EMRGAGFAYPGAAEPVLRDVTLTARPGTTTAIVGSTGAGKTTLLGLVPRLFDAASGAV
                     LVDGVDVRDLEPEHLWSRVGIVPQKPYLFSGTVASNLRYGNPDATDEQLWGALEVAQA
                     AEFVRAMPERLAAPIAQGGTNVSGGQRQRLAIARLLVRAPQIYLFDDSFSALDLATDA
                     RLRAALRPVTATATVLVVAQRVASIRDADQIVVLDDGAVVGIGTHDELLATCSTYAEI
                     VASQFSAEEMSAEEVV"
     gene            complement(3026..3679)
                     /locus_tag="VFE39_12240"
     CDS             complement(3026..3679)
                     /locus_tag="VFE39_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6259412.1"
                     /translation="MVTPSGRAPRMAPDARRAAIIAATLPLVLRHGAAVTTRQIAQAA
                     GIAEGTIFRVFPDKESVVQAVVAEAFDPAPTLRELAAVDCELPFRERLTAAVTVLQDR
                     IAGVFGLLNALGWTGPPDREGRPAAARHPPGTANPTEINDAFRAAIVELIGSDECRLR
                     VPAAELAHVLRLLVFSGTHPMIADGHQLEPEQIVAILLDGLAAPLPAPPDTEEDRPC"
     gene            complement(4142..5002)
                     /locus_tag="VFE39_12245"
     CDS             complement(4142..5002)
                     /locus_tag="VFE39_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6259413.1"
                     /translation="MFQARLHDGTSIDVEVRGAGPTVLLPVNPRPVEGPQADAMRRWG
                     ADPALGRALVDGLADSFRVVAFDYEGHVLATPKPDTLTPANVAGDILAVADAAGAERF
                     AYYGYSWLALCGLQLAIRTDRLWALAMGGYPPIDGPYAEMLQVTAAAHRMSTEVDAVP
                     TDFSSEGTDWSSVEVTTSERQTRQFMTLYAALQAFDDRAAQRHITCPRLCFAGSADEI
                     EYGERWGGVRVRMAGPLVGRRSELEGHGWEVHVIDGLDHTGAMQATHVLPILRPWLAA
                     ARPAVPLRRE"
     gene            5082..>5886
                     /locus_tag="VFE39_12250"
     CDS             5082..>5886
                     /locus_tag="VFE39_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET6259414.1"
                     /translation="MAVFAGQGDAARSMPLLWREKGAAGHRHGPGPKPGLTVDAVVSA
                     AIGVADDANVAGLSMRAVAERLGVTAMALYTYVPGKNELVDLIYDGVHAELPTDGRPA
                     SGWRAAVTAWAEDLVALYVRHPWALQVSFARPVLGPHEQAVLEALVRILRETGAGAEV
                     LRRVVSVLFHLVRGTARTIAEARIAPTAGSTSEQEWWAGRAAQLAEVVPDFAERFPQT
                     MWLAGAGAARTGDGQTPYLEHEAREALAVGLAVLLDGLEAGTIAVPSRGR"
BASE COUNT          901 a         2110 c         2047 g          828 t
ORIGIN      
        1 ctcgaagacc acacgaccgc ggcgggtgga cggcaccgcg ggcaccgccg gatcgggcac
       61 ctgctcctcg gcgtcgagca gttcgaacac gcgctcggcc gaggcgatcc ccgattgcag
      121 gacgttggcc atggaagcca gctgggtgat gggctgggtg aactggcggg agtactggat
      181 gaacgcctgg acgtcaccga ggctcatcga cccggacgcc acccgcagcc cgccgaccac
      241 ggccaccagc acgtagttca ggttcccgat gaacatcatc gacggcatga tgatcccgga
      301 gatgaactgc gcgcggaagc tggcgtcgaa cagccgctcg ttcttctccc cgaagtccgc
      361 ctccacctcg cggcgccgcc cgaacacccg caccagctcg tgaccggtga acgcctcctc
      421 gatgtgggcg ttcagagcgc cggtgtgccg ccactgcgcc acgaactgct tctgcgaccg
      481 cttcgcgacg gcccgcgtga ccagcaccga caccgggatc gtgacgagcg cgaccagcgc
      541 cagcagcggc gagatcagca ccatcatcaa cagcacgccg accacggtga gcagcgacgt
      601 cagcagctgg ctcatcgtct gctgcagggt ctgggagatg ttgtcgatgt cgttggtgac
      661 gcggctgagt acctcgcccc gctgctgccg gtcgaagtag cgcagcggca gccggttgag
      721 cttctcctcc acctcgcggc gcagcccgaa gatcgtgcgc tgcacgatcc cgttcagcat
      781 gtacccctgc agccagccga acagggaggc tgcgacgtag atcgcgatga cgccgagcag
      841 cacctggctc agcaacgcga agtcgatgcc ctgtccgggc acgacgcccg agccctggat
      901 gacgttggcg aacgtctcgt cgcccgcggc gcgggcggcg gcggccgctt ctgcggtgct
      961 ggtgccaggt gggatttgcc gcccgatcac accggcgaag atgacgtcgg tggcatggcc
     1021 aaggatcttc ggaccgatca ccgacagcgt cacgctgatg atgccgagga ccagaacggc
     1081 gaccgccccg gcccgctccg gcgcgagcct gccgatcagc cggcgcgccg acggaccgaa
     1141 gtccatcgac ttctcggccg gcatcccagc tgccccccac cgcgggccac cctgacgcgt
     1201 ggcggccatt tcgcgtgccg cgcgctgggt gtcgtcgctc ccctcgcccg gggggacggg
     1261 cgtcggggac gtgctcggcc tggtcatacc acttcctccg cgctcatctc ttcggcgctg
     1321 aactgggacg cgacgatctc ggcgtacgtc gagcaggtcg cgagcagctc gtcgtgcgtc
     1381 ccgatgccga cgacggcgcc gtcgtcgagc acgacgatct ggtcggcgtc gcggatgctg
     1441 gccacccgct gggcgaccac cagcaccgtc gccgtcgccg tgacgggtcg cagtgccgcg
     1501 cggagccggg cgtccgtggc caggtcgagc gcggagaacg agtcgtcgaa cagatagatc
     1561 tgcggcgcac gcacgagcag tcgcgcgatc gcgaggcgtt gccgctggcc gcccgagacg
     1621 ttcgtgccac cctgcgcgat cggcgccgcc agtcgctcgg gcatggctcg tacgaactcg
     1681 gcggcctggg ccacctcgag tgccccccac agctgctcgt ccgtggcgtc ggggttgccg
     1741 taccgcaggt tgctcgcgac ggtgcccgag aacaggtagg gcttctgcgg cacgatcccg
     1801 acccggctcc agaggtgctc aggttcgagg tcgcgcacgt cgacgccgtc gacgagcacc
     1861 gcgccgctcg cggcgtcgaa cagccggggc acgagcccga gcagggtggt cttgcccgct
     1921 ccggtgctcc cgacgattgc ggtggtcgtc ccgggccggg ccgtgagcgt gacatcgcgc
     1981 agcacgggct cggcggctcc ggggtaagcg aagccggcgc cgcgcatctc cagcacgccc
     2041 gtggccgccc gggggacgac cggccgctcc ggcagcgaga ccgacgactc cgtgtcgagc
     2101 acctcgatga cccgctcggc gcagacggcc gcgcggggga tcatcatcac catgaatgtg
     2161 gccatcatga ccgccatgag gatctggatc aggtagctca ggaacgcggt gagcgccccg
     2221 atctgcatct gcccggcgtc gatccggctc gcgccgaacc agagcacagc cacgctggag
     2281 acgttgagca cgagcatgac gatcgggaag atcatcgcca tcaggcgccc gacccgcgtc
     2341 gcgacgtccg tcaggtccgc gttggcccgc gcgaaccgcg ccgtctcctc cggctcccgg
     2401 acgaatgccc gcaccacccg gatgcccgtg atctgctcgc gcagaacgcg gttcacctcg
     2461 tcgatccgca cctgcatgct gcggaagccc ggcaccatcc gcacgatcac cgcgccgatc
     2521 gcgagcacca gcaccgggac acagatcagc atcagcctgg acagctgagc gtcctcctgc
     2581 agggccatga ccacgccgcc gaagcacatg atcggcgctg tcaccagcat cgtgcaggac
     2641 agcagcacaa gcatctgcac ctgctggacg tcgttggtcc cccgggtgat cagcgacggg
     2701 gcaccgaacc ggttgacctc gcgcgcggag aactccccca cccggtggaa cagcgccgcg
     2761 cgcacgtcgc ggccgaaccc catggcggtg cgcgcgccga cgtacaccgc ggcgatcgag
     2821 cacgcgatct gcacgagcgc gacgagcaac atccacccgc cggtgcccac gatgtagtcc
     2881 gtgtcgccct tggcgatgcc ctggtcgatg atctcggcat tgaggctcgg caggtagagc
     2941 gaggccatcg tgccgaccag ctgcagcatg acgaccagca gcaacggccg ggtgtagggg
     3001 cgcaaccgct cgcgcagcag gcggatcagc atggacggtc ttcctctgtg tcggggggtg
     3061 cggggagcgg ggcagcgaga ccgtccagca ggatcgcgac gatctgctcg ggctccagct
     3121 ggtggccgtc ggcgatcatc ggatgagtgc cggagaagac gagcagccgc aggacgtgcg
     3181 cgagttcggc ggccggcacg cgcagtcggc actcgtcgga gccgatcagc tcgacgatgg
     3241 ccgcacggaa ggcgtcgttg atctcggttg ggttcgcggt ccccggcggg tgccgcgccg
     3301 ccgccggccg cccctcccgg tccgggggtc cggtccaacc cagggcgttg agcaggccga
     3361 acacccccgc tatgcggtcc tgtagaaccg tgacggccgc cgtgaggcgc tcgcggaacg
     3421 ggagctcaca gtcgacggcc gccagctcgc gcaaggtggg ggccgggtcg aacgcctccg
     3481 ccaccactgc ctggaccacc gactccttgt ccgggaagac ccggaagatc gtgccctcgg
     3541 cgatcccggc ggcctgggcg atctgccggg tggtcacagc ggcgccatgg cgcagcacga
     3601 gcggcagcgt ggcggcgatg atcgcggccc gacgcgcgtc gggcgccatg cggggggcgc
     3661 ggccgctcgg agtcaccatg ccggcgaccg taaatgagcg cgcactcact agcaaacgcg
     3721 ttccgttccc gccctgctac tgagtgacat gcggacgatc cggttgttcg cgcacagtta
     3781 cgtaccccgc ggccgccggt cccgtcagcc gcgtcggggt gggcgggatg gagctcgaca
     3841 gcatctccga gacgccgctg gacgggttgc acaccctggt cgaaacgacg tggaccgtcc
     3901 acgtcgaccc cagggagacg acgccgaacc cttgacgctg gcgtcgccgt tcctgctgcg
     3961 ccgccacgag gaacgatggc ggatcgtcct ctacctcaac caccatgaca tcgcgagcat
     4021 catcagcacc cggcgcccgg cgctcggtgt aaccatcacg tcggccgctg ccaccacttc
     4081 tgcgcctgga cggggctcgc gcgtggacgc ccggctcgcg cacccctcga ggtctgggga
     4141 cttactcacg tcgaagcggc acggcgggcc gggcggccgc gagccagggc cgcaggatcg
     4201 gcaggacgtg ggtggcctgc atggctccgg tgtggtcgag cccgtcgatg acgtgcacct
     4261 cccagccgtg gccctccagc tccgagcgcc gcccgaccag cgggccggcc attctcaccc
     4321 gcacgccgcc ccagcgctcc ccgtactcga tctcgtccgc cgagccggcg aagcagaggc
     4381 gcggacacgt gatgtgccgc tgggcggcgc ggtcgtcgaa ggcctgcagg gccgcgtaca
     4441 gcgtcatgaa ctggcgggtc tgccgctcgc tcgtcgtcac ctccaccgat gaccagtcgg
     4501 tcccctccga actgaaatcc gtcggcacgg cgtccacttc ggtggacatc cggtgcgccg
     4561 ccgccgtcac ctgcagcatc tcggcgtacg gcccgtcgat cggcgggtac ccgcccatgg
     4621 cgagcgccca cagccggtcg gtgcgtatcg cgagctggag accgcagagc gcgagccagg
     4681 agtacccgta gtaggcgaac cgctcggcgc ccgcggcgtc ggccacagcc aggatgtcgc
     4741 ctgcgacgtt ggccggcgtc agcgtgtccg gcttgggagt ggcgaggacg tggccctcgt
     4801 agtcgaaggc gaccacccgg aacgagtccg ccagcccgtc gacgagcgct cggccgagtg
     4861 ccggatccgc gccccaccgg cgcatcgcgt cggcctgcgg cccctcgacc ggtcgaggat
     4921 tcacgggcag caacaccgta ggaccggcgc cgcgaacctc gacgtcgatg ctcgtcccgt
     4981 catgcagccg tgcctggaac atcgatattc cccctgcgaa ctctttacga cgtatagcaa
     5041 tggccgcgct accctagcgt acgacgtaag gagttgtctt tgtggctgtt ttcgccggtc
     5101 agggcgatgc cgcccggtcg atgcccctgc tgtggcggga gaagggcgcg gccgggcacc
     5161 gacacggtcc cggccccaaa cccggactga ccgtcgacgc ggtcgtgtcg gcggcgatcg
     5221 gggtggccga cgacgcaaac gtggccggac tgtccatgcg tgcagtggcc gagcggctcg
     5281 gcgtgacggc catggcgctc tatacctatg tgccgggcaa gaacgagctc gtcgacctga
     5341 tctacgacgg cgtccacgcc gaactgccca ccgacggccg gcctgcctcg gggtggcgcg
     5401 ccgccgtcac ggcgtgggcc gaggacctcg tcgcgctgta cgtgagacat ccctgggccc
     5461 tgcaggtgtc gttcgcgcgg ccggtgctcg gcccgcacga gcaggccgtc ctcgaggctc
     5521 tggtgcggat cctgcgcgag acgggtgcgg gcgccgaggt gctgcgccgc gtcgtgagcg
     5581 tgctcttcca cctcgtgcgc gggacggcca ggacgatcgc ggaggcgcgt atcgcgccga
     5641 cggcgggcag cacctccgag caggaatggt gggccggccg ggcggcacag ctggccgagg
     5701 tcgtgcccga cttcgccgag cgcttccccc agacgatgtg gctcgcgggc gcgggtgcgg
     5761 cgcggaccgg ggacggccag acgccctacc tggaacacga ggcacgcgag gccctcgcgg
     5821 tcgggctcgc agtgctgctg gacggcctcg aggcgggcac catcgcagtc ccgtcccgag
     5881 gccggt
//
LOCUS       DASRSC010000250         4491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_260158,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000250 DASRSC010000000
VERSION     DASRSC010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260158"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VFE39_12255"
     CDS             complement(<1..644)
                     /locus_tag="VFE39_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET6259415.1"
                     /translation="MTATWPAPLAPGPVRGRVAVPGSKSVTNRALLLAALSGGAAVVA
                     GAPATRDTALMVGAMRALGVPLEVDGERVAFAPHDGLRGGGAVDCGLAGTVMRFVPPA
                     AALADGPVAFDGDPRAQERPMGTVLDALRALGAQVDGDRLPFTLHGTGALPGGDVVID
                     ASASSQFVSGLLLSGARYDKGVTVHHDGKPVPSLPHIEMTVDMLRAAGVDVDDT"
     gene            complement(680..1576)
                     /locus_tag="VFE39_12260"
     CDS             complement(680..1576)
                     /locus_tag="VFE39_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6259416.1"
                     /translation="MDVRLQDVRYFIAVAEELSFTRAAERLFVSQPALSKQIRQLETQ
                     LRAELFTRRPRQVALTAAGQALLPRARRLIAEWDGAVEDVRAAATADERMLTVGFHTR
                     IGRGLVPAITATMGARLPGWGLQFRQVPWDDPAVGLAGGGVDVAVAWLPVAAGLSTRI
                     IATEDRWVTLPVGHRLADRPAVRWSDIQDEPFVALPASAGPMRDFWLAADRRTSPPRI
                     AAEAATADESFEVVAAGCGVVLVAAGNAEIYGRDDLVHIPVLDLPPAELAVAWRTGDR
                     RTAVRVVVDTCFQCAAAGPAAS"
     gene            1638..2546
                     /locus_tag="VFE39_12265"
     CDS             1638..2546
                     /locus_tag="VFE39_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6259417.1"
                     /translation="MRAIVHDPAAGLRHTDVPDPDPRPSELLLDVRAAALNFLDVAYH
                     DELFAPGAVLGVDAAGVVLAPAADGSGPPAGSRVVTFALGGAWAQRRAAATADAAVVP
                     DGVDLAVAAALPAAGVTALHAVRRLGPLLGRRVLVTGASGGVGRYAVQLAARAGADVV
                     AAVGNPGRACGLVELGARQVVTDLADVTMPVHGVLDNVGGPLLAEAFALLAEDGLALS
                     IGQASRQPTTIDFEQERRRGGGRRLEPFTVPSGIGPDLAVLLDLVERGQLDSQIGWRG
                     SWLRAADAAEALLARQVPGKAVLEIG"
     gene            2552..3391
                     /locus_tag="VFE39_12270"
     CDS             2552..3391
                     /locus_tag="VFE39_12270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET6259418.1"
                     /translation="MELGVHGLNAKAAVSPAVTARLARLAEELGYRSWWVGEHVVVPS
                     PRTPATPIDPRDPMLDPLVHLAYVAALTSQIELGTAVIILPQRNPVVLAKQAASLDVL
                     SGGRLLLGVGAGYLEPEMTAVGVPMADRGARTDEYLDAMTELWGSPAPAYKGRFVGFA
                     DVDAHPRPERPGGPRIVVGGRSRAAYRRAVTRGHGFFGTGSVEDLAGDIAGLRRTVAE
                     VERPARLGRLEITAMPLGPVDSATATRYADLGVDRLVVYPLPFEDPDDIARFLEHHAD
                     LAR"
     gene            3411..4205
                     /locus_tag="VFE39_12275"
     CDS             3411..4205
                     /locus_tag="VFE39_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HET6259419.1"
                     /translation="MCGRYASIKAPADLADEFRAVDATDGAATADYNVAPTKQIVTVV
                     ERHPRDVDGTPDPDRTERTLRMVRWGLVPSWAKDPKAGARMINARSESAATKPAFRRA
                     LNARRCLIPADGWFEWQRGPEHKQPYYTHYSDGSSLAMAGLWEFWKPKDDPDGQYPDG
                     LVTATVLTTEAVGPLAQVHDRMPLVLPASAWDAWLDPDAHADSDAVAALLRPPSEQLV
                     ANLELRPVSPLVNSVRNNGPELLDPLSPRDVREPLQLDLLAGPNPK"
     gene            4291..>4491
                     /locus_tag="VFE39_12280"
     CDS             4291..>4491
                     /locus_tag="VFE39_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005440698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6259420.1"
                     /translation="MSTEVGTPHGPARIARHDADGDATAVLLLGHGAGGGVGAPDLLA
                     AAGTAVAAGVHVVLVEQPYRVAG"
BASE COUNT          587 a         1652 c         1687 g          565 t
ORIGIN      
        1 tccgtgtcgt cgacatccac gccggcagcc cgcaacatgt cgacggtcat ctcgatgtgc
       61 ggcagcgacg gcacgggctt gccgtcgtgg tgcacggtga cccccttgtc gtagcgggca
      121 ccggacagca gcagcccgga cacgaactgg gatgacgccg atgcgtcgat caccacgtcg
      181 ccgccgggga gcgcaccggt gccgtgcagc gtgaacggca gccggtcgcc gtccacctgc
      241 gcacccaggg cgcgcagcgc gtcgagcacc gtgcccatcg gccgttcctg tgcccgcggg
      301 tcgccgtcga acgcgaccgg gccgtcggcc aatgccgctg cgggcggcac gaaccgcatg
      361 acggtgccgg cgagcccgca gtccaccgct ccgccgcccc gcaggccatc gtgcggggcg
      421 aaggccaccc gctcgccgtc cacctcgagc gggacgccga gcgcgcgcat ggcgccgacc
      481 atcagcgccg tgtcacgggt ggctggggcc ccggccacga ccgccgcacc tccggagagg
      541 gcggccagca gcagggcccg gttggtcacg gacttggacc cgggtacggc cacgcggccg
      601 cgtacggggc cgggagcgag cggcgcgggc caggtggcgg tcacgccgca atgattcccc
      661 atggccgcca gcatgccggt caggaggccg ctggccccgc cgccgcgcac tggaaacagg
      721 tgtcgacgac cacccgtacc gccgtgcgcc ggtcgccggt gcgccaggcg acggcgagct
      781 cggcgggcgg caggtcgagc acggggatgt gtacgaggtc gtcccggccg tagatctcgg
      841 cgttcccggc cgccaccagc accacgccgc agccggctgc gacgacctcg aaggactcgt
      901 cggccgtggc ggcctcggcc gcgatccgcg gcggggacgt ccgccggtcg gcagcgagcc
      961 agaagtcgcg catcgggccg gccgaggcgg gcagcgcgac gaacggctcg tcctggatgt
     1021 cgctccaccg cacggcgggc cggtcagcga gccggtggcc gaccggaagc gtcacccagc
     1081 ggtcctcggt ggcgatgatc cgcgtggaga ggcccgcggc gaccggcagc cacgcgacag
     1141 cgacgtcgac cccgccgccc gcgagaccca ccgccgggtc gtcccacgga acctgccgga
     1201 actgcaaccc ccaccccggg agtcgcgcac ccatcgtcgc ggtgatggcc gggacgagcc
     1261 cccggccgat gcgggtgtgg aagccgacgg tgagcatccg ttcgtcggcg gtcgcggcgg
     1321 cccggacgtc ctcgaccgca ccgtcccact cggcgatcag tcggcgggcg cgtggaagga
     1381 gtgcctgccc tgctgccgtg agcgccacct ggcggggtcg ccgggtgaac agctccgccc
     1441 gcagctgcgt ctccagctgc cggatctgct tgctcagcgc cggctgcgag acgaacaggc
     1501 gctcggcagc ccgggtgaag ctcagctcct cggccacggc gatgaagtag cgcacatcct
     1561 gcaggcgcac atccatagcc acaggttatg gcaacaggtc ttggacgcac gtctgtcggg
     1621 ggcgaacgct cgtggatgtg cgagccatcg ttcacgatcc cgccgcaggg ctgcggcaca
     1681 ccgatgtccc cgaccccgat ccccgcccca gcgagctctt gctcgacgtg cgggccgcag
     1741 cgctgaactt cctcgacgtc gcctaccacg acgagctctt cgctcctggc gccgtgctcg
     1801 gtgtcgacgc cgccggggtg gtgctcgcgc cggcggcgga cggttccggg ccgcccgcag
     1861 gcagtcgcgt cgtcacgttt gctctgggcg gggcgtgggc gcagcgccgg gccgcggcca
     1921 ccgcggacgc ggccgtcgtg ccggacgggg tggaccttgc cgtggcagcg gcgctgccgg
     1981 cggccggggt caccgcgctc cacgccgtac ggcggctggg tccgctgctc ggccgccgcg
     2041 tgctcgtgac cggggcgtcc gggggcgtgg gccggtacgc cgtccagctc gcggcgcggg
     2101 ccggggccga cgtcgtcgcg gcggtcggta acccggggcg tgcgtgcggc ctggtcgagc
     2161 tgggggcgcg gcaggtggtc accgacctgg ccgacgtgac gatgcccgtg cacggcgtgc
     2221 tcgacaacgt cggtggtccg ctgctcgcgg aggccttcgc gctgctcgcc gaggatggcc
     2281 tcgcgctgag catcgggcag gcgtcccggc aaccgaccac gatcgacttc gagcaggagc
     2341 gcaggcgcgg aggcggcagg cggctggagc cattcaccgt cccgtccggc atcggccccg
     2401 acctggcggt gctgctcgac ctcgtcgaac gcgggcagct cgattcccag atcggctggc
     2461 gcggctcctg gctccgtgct gccgacgccg cagaggcact gctcgcccgg caggtgccgg
     2521 gcaaggccgt actcgagatc gggtgaccgc gatggaactg ggtgtccacg gtttgaacgc
     2581 gaaggcggcg gtgtcgcccg cggtgacggc ccgcctggcg aggctcgcgg aggagctcgg
     2641 gtaccggtcg tggtgggtgg gcgagcacgt cgtggttccg agcccgcgca cgccggcgac
     2701 accgatcgat ccgcgcgacc cgatgctcga cccgctcgtg cacctggcgt acgtggccgc
     2761 cctgaccagc caaatcgagc tgggcaccgc ggtgatcatc ctcccgcagc gcaaccctgt
     2821 ggtgctggcg aagcaggccg cgagcctcga cgtgctgagc ggcgggcggc tgctgctcgg
     2881 cgtgggggcg gggtacctgg agccggagat gacggcggtg ggcgtcccga tggcggatcg
     2941 cggcgcgcgc accgacgagt acctcgacgc catgaccgag ctctggggct cgcccgcacc
     3001 cgcctacaag ggccgcttcg tcgggttcgc cgacgtggac gcgcacccgc ggccggagcg
     3061 gccgggcggg ccacggatcg tcgtcggtgg ccgcagccgg gccgcctacc gccgggcggt
     3121 cacccgcggc cacgggttct tcggcaccgg atcggtcgag gatctggccg gggacatcgc
     3181 gggactgcgc cggacggtgg cggaggtcga gcggcccgcg cggctcggcc ggctggagat
     3241 cacggcgatg ccgctggggc ctgtcgattc cgccaccgcc acccgctacg ccgacctggg
     3301 cgtcgaccgg ctcgtcgtct acccgctccc gttcgaggac cccgacgaca tcgcccggtt
     3361 cctggagcac cacgccgacc tcgcgcgctg atcggtgcga cgatggggac atgtgcggcc
     3421 gatacgcctc catcaaggcc cccgccgacc tcgccgacga gttccgggcg gtcgacgcca
     3481 ccgacggcgc tgcgacggcg gactacaacg tggcgcccac caagcagatc gtcaccgtcg
     3541 tggagcggca cccacgcgac gtcgacggca cgcccgatcc ggatcgcacc gagcgcacgc
     3601 tccggatggt ccgctgggga ctggtgccgt cctgggccaa ggaccccaag gcgggcgccc
     3661 gcatgatcaa cgcccggtcg gagtcggcgg cgacgaagcc cgcgttccgc cgggcactga
     3721 acgcgcggcg gtgcctgatc cccgccgacg gctggttcga gtggcagcgg gggccggaac
     3781 acaagcagcc gtactacacc cactactccg acggcagctc gctcgcgatg gcggggctct
     3841 gggagttctg gaagccgaag gacgaccccg acggccagta ccccgacggg ctcgtgacgg
     3901 ccacggtgct gaccaccgag gcggtcggac cgctcgcgca ggtgcacgac cggatgccgc
     3961 tggtgctgcc ggcctcggcg tgggacgcct ggctcgaccc ggatgcgcac gcggactccg
     4021 acgcggtggc ggcgctgctg cggcccccgt cggagcagct ggtggcgaac ctggagctgc
     4081 ggccggtgtc gccgctggtc aacagtgtgc gcaacaacgg ccccgagctg ctcgacccgc
     4141 tctcgccgag ggacgtccgc gagccgctgc agctggacct gctggccggg ccgaacccga
     4201 agtgagcccg gtgatcgcca taggtcgcac accagcgcca cgatcggggc gctcccgtgc
     4261 ggtccgtgcc gatcacgtcg gggcgcggcc gtgagcaccg aggtcgggac gccgcacggc
     4321 cccgcccgga tcgcccggca cgacgccgac ggcgatgcca cggccgtgct cctgctcgga
     4381 catggcgcgg gcggtggggt gggcgcaccg gacctgctcg ctgcggcggg caccgctgtc
     4441 gcggccggag tacatgtcgt gctggtcgag cagccctacc gcgtcgccgg t
//
LOCUS       DASRSC010000251        16195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3697213,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000251 DASRSC010000000
VERSION     DASRSC010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 16195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16195
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3697213"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /gene="obgE"
                     /locus_tag="VFE39_12285"
     CDS             complement(<1..660)
                     /gene="obgE"
                     /locus_tag="VFE39_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET6259421.1"
                     /translation="MSKFVDRVVLHATAGAGGNGCASVHREKFKPLGGPDGGNGGRGG
                     SVVLVVDPGVHTLLDFHHRPHAMARNGKQGQGGFRAGANAEDLELQVPDGTVVFDTEG
                     EIIADLVGVGTRFVAAEGGRGGLGNAALASAARKAPGFALLGEPGENRDLVLELRSMA
                     DVGLVGFPSAGKSSLVAALSAARPKIADYPFTTLEPSLGVVRVDDTTEFVVADVPGLI
                     EG"
     gene            complement(739..996)
                     /gene="rpmA"
                     /locus_tag="VFE39_12290"
     CDS             complement(739..996)
                     /gene="rpmA"
                     /locus_tag="VFE39_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET6259422.1"
                     /translation="MAHKKGASSSRNGRDSNAQRLGVKRFGGQVVGAGEIIVRQRGTK
                     FHPGTGVGRGGDDTLFALVQGAVEFGYTRGRKVVNIVPASV"
     gene            complement(1009..1317)
                     /gene="rplU"
                     /locus_tag="VFE39_12295"
     CDS             complement(1009..1317)
                     /gene="rplU"
                     /locus_tag="VFE39_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET6259423.1"
                     /translation="MYAIVKTGGKQYKVAVDDVLTVEKIDGKPGAEVSLPALLLVDGD
                     DVTTDAEALAAASVTATVVEHTKGPKIRIHKFKNKTGYHKRQGHRQPLTKVQVTGIAK
                     "
     gene            complement(1449..4685)
                     /locus_tag="VFE39_12300"
     CDS             complement(1449..4685)
                     /locus_tag="VFE39_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HET6259424.1"
                     /translation="MSNNDSPAGTTGGPVTVPVLDDLPAKMRVHALAKVLGRTSREVL
                     AVLADLGEAGRSAQSSIDRKVAEQVVTALVPATAARSADPEPAAEPAEAAVEEAPVEE
                     AAPLAPVFAPPTPLFQPPTRPARTRRRGAEPEATPVSDETELSPVSTDAPVEERVAEP
                     APAATDGDDSDDSDDGARRRRRRGRRGRGRGRGSDGFDDESGDDSDDSDDSDEAVSVD
                     TTAAGAAGAGAESGSDQDEDSDRESDDEAGGSRRRRRRRRRRGSADDGADTADTADEP
                     DPPNTVVRVRQPREQRSDDSADGGGDGVQSVRGSTRLEAKRQRRRDGRDAGRRRVPIL
                     SEAEFLARRESVDRSMVVRERGDRVEIAVLEDDVLVEHFVTDHGGTGPSMVGNIYLGR
                     VQNVLPSMEAAFVDIGRGRNAVLYAGEVDWDAAGLGGKARRIEQALSSGDSVLVQVTK
                     DPIGHKGARLTTQISLAGRFLVYVPSGGAAGISRKLPDVERKRLKDMLKEIVPSEAGV
                     IIRTASEGVSHEALERDVRRLQAQWEVVKTKAEQTGPNKPKAPVLLYEEPDLLLKVVR
                     DQFNEDFTRLIAEGDDAWDTLQNYVGHVAPELADRLHRHVGPSDVFAEYRIDEQLLKG
                     LDRKVWLPSGGTLVIDRTEAMTVIDVNTGKFTGSGGNLEETVTRNNLEAAEEVVRQLR
                     LRDIGGIIVIDFIDMVLEANRDLVLRRLTECLARDRTRHQVAEVTSLGLVQMTRKRVG
                     GGLLEHFSTPCEHCRGRGVIVNAEQTLEKGDSGHDRSDRGDRSDRDAPGRRSRSRGRR
                     GDSEERNGHGDQHSGGQYNGGQQNGGQQNGVRVDTNTSDAEVGVGAPAPVVEAAYPVV
                     VEAPAVADVPVAEAPDAPVIDAPAVEPAVVEVPVPTGRRTGGRRIARRGVVETSPQAR
                     PVDVVADAVEEVVVERSIAEQAAPAAASPTAAAVADSAVADSPVAGSAVAGPPVADSP
                     VVASPEAGSAAADSEQHAQPRPRQRRGRVTRTAGAPAPVAGDAPVAAVVTVPAARTAS
                     SEPATPVAAPPQPAPTGATRPRRTRRAASRPAGPPVTDGTGESGGDPV"
     gene            complement(4875..5678)
                     /locus_tag="VFE39_12305"
     CDS             complement(4875..5678)
                     /locus_tag="VFE39_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HET6259425.1"
                     /translation="MARVRPGEAANPAPPTVQRIRLRFAKRGRLRFLSHRDVARSFER
                     AVRRAGVPVAHSHGFSPHPRLSWIGAAPTGSASEAEYLEIGLTREVDPAALVTALDAA
                     LPDGLDVLDAAVACGTALAERIDGSRWVIELPGVAPDALRAALAALLAEESIVVERVT
                     PSGRRQIDVRAALVHAEVSRDANAPGATSGGSLSTPPDQGAVCAIMTAVVRHTTPAVR
                     PDDVLGALDVVAGLKSPVPAKATRMAQGLLDHRGDLADPLGSDRVTTRA"
     gene            6030..6164
                     /locus_tag="VFE39_12310"
     CDS             6030..6164
                     /locus_tag="VFE39_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259426.1"
                     /translation="MRAFTGAGLGVSMRVLLDAAQEPGGDPAQALDEALALLDAGLPL
                     "
     gene            complement(6170..6688)
                     /locus_tag="VFE39_12315"
     CDS             complement(6170..6688)
                     /locus_tag="VFE39_12315"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6259427.1"
                     /translation="MSDRAGSGPQVGIRVADERDVAALARLRRTWLEERTGRPVEDPG
                     FEAAFEQWWRVELPRRTFWLAEVGSARSGFTAVGSINVVEIVHMPRPGARSGRIGHVG
                     NAFVLASFADGGVPAALLAAALEHARDRRYRRLLLAPTGGSTAFYRRAGFVPAGEQML
                     MLDPSTLPTPPQ"
     gene            complement(6681..8636)
                     /locus_tag="VFE39_12320"
     CDS             complement(6681..8636)
                     /locus_tag="VFE39_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HET6259428.1"
                     /translation="MTVPSVYPDLEPLLPRVSKPVQYVGGELNAQLKDWESAAVRWAL
                     MYPDAYEVGLPNQGLMILYEVLNERVDALAERCYAVWPDLEALMREHGVPAFTVDGHR
                     PLGAFDLLGVSFSTELGYTTLLTALDLAGIPLHAIDRGIDHPVVVAGGHAAFNPEPIA
                     DFVDVAAIGDGEEVVGEITGVVKEWKAAGRPGGRRELLLRLAATDGCYVPSLYAVSYG
                     DDGEIASVAPVDERVPRSVTKRTTTDLDAWPYPKKPLVPMAETVHERASVEIFRGCTR
                     GCRFCQAGMITRPVRERTLAGIGEMVDSAVRASGFDEVGLLSLSSADHSEIAEITKGL
                     ADRYEGTNTSLSLPSTRVDAFNIDLANEISRNGRRSGLTFAPEGGSERIRRVINKMVS
                     EEDLIRTVSEAFAQGWRQVKLYFMCGLPTEEDEDVLEIAGMAHRVIQAGRAASGHNDI
                     RCTISIGGFVPKPHTPFQWAAQAHPEVVDERLRKLRAAVNSNRKLGRNVGMRYHDGQP
                     SLIEGLLARGDRRVGRVIERVWRDGGRFDGWSEHFSYERWTGAAAAELEPLGVSLDWF
                     TTRERGRDEVLPWDHLDSGLERDWLWDDWQDALSGREQDDCRWTPCFDCGVCPSTGTD
                     IEVGPSGTTLLPLTVVNPVSGGPGTSV"
     gene            complement(8776..9567)
                     /locus_tag="VFE39_12325"
     CDS             complement(8776..9567)
                     /locus_tag="VFE39_12325"
                     /inference="COORDINATES: protein motif:HMM:NF015060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HET6259429.1"
                     /translation="MTVTFQRPVLADLIRNAVVVLLAVLQLVVSGLGGIGLAGDTAGA
                     VAHEYASPLLAAGWAFAIWAPIYAGFLAYAVYQVLPAQRGRQIHRETGWWLAASAVFN
                     IAWVLAFEAGWLPLAELLILALLVTLAVVFGRLSRQPADSVLERVVLRGTVALYTGWV
                     SFAVALCTAATGVWVGLPGTGALAAIAAVVVLLAVSVIASWVVLSGTAVVGYAIAAVW
                     AAAGIALNDPPAGVVVAGAIAIVTVIAAATRRLTTAGNPVRAAWG"
     gene            complement(9656..10486)
                     /locus_tag="VFE39_12330"
     CDS             complement(9656..10486)
                     /locus_tag="VFE39_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6259430.1"
                     /translation="MTAQVPAPPAPPALLRLIPAGSYAGRSRALLLRSATASRRAWLA
                     FVSGFFEPVFYLVAMGQGLGSLVGVLPGPDGAPISYAAFIAPGLLAASAMNGAVFDST
                     FNVFFKLKYGRLYDAMLATPLGPVDIALGEIGWALIRGGLYALGFLSVMAGFGLLVSP
                     WALLALPAALVVAFAFAAVGMAATSYMRSWQDFDLVTLAILPMFLFSTTFYPLAVYPR
                     WLQLVVEFLPLFHAVELMRGLTTGAVHLGMVGHLAYFLVMIVVGVMVASRRLDVLLLR
                     "
     gene            complement(10483..11256)
                     /locus_tag="VFE39_12335"
     CDS             complement(10483..11256)
                     /locus_tag="VFE39_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6259431.1"
                     /translation="MLLVVEHFWTWYRRNWRATAVSSVLQPLLFLLAFGVGFGSLVAG
                     TGSAAAATGGVDYIVWLAPALLAVSTVQSAAFESTYPVLSGFKWQRTYFAMTAGPISP
                     AQVALGHLLWVVCKMAGAGAIYIGVIAVFGGVTGPGIVVSLMVAVLTGAAVAALVTAF
                     SATVENEGAAFNALFRFVVIPMTLFSGTFFPVDRLPGWVQPVALISPLWHGTELARSA
                     ALGAVPQPLAALGHLAYLCALLVLGLALATRFFARRLAQ"
     gene            complement(11367..12320)
                     /locus_tag="VFE39_12340"
     CDS             complement(11367..12320)
                     /locus_tag="VFE39_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6259432.1"
                     /translation="MSGLAASVLFVADGLVQARGLRKRFGDFEAVRGVDVDVARGEVF
                     GFLGPNGAGKSSTMRMIACVSPRSDGALQVLGMDPDVDGPRIRARIGVVPQLDNLDTE
                     LTVRQNLQIYGRYFGLSRAHVRAKATELLEFAQLTERADDSVEPLSGGMKRRLTIARA
                     LVNEPELLLLDEPTTGLDPQARHLLWERLYRLKREGVTQIITTHYMDEAEQLCDRLVV
                     MDGGVIVAEGSPADLIERYSTREVLELRFDPALRPGVDALALLAERVEELPDRLLLYT
                     ADGEHTHAEVARAGFVPLSALVRRSSLEDVFLRLTGRTLVD"
     gene            12376..12954
                     /locus_tag="VFE39_12345"
     CDS             12376..12954
                     /locus_tag="VFE39_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET6259433.1"
                     /translation="MAVRTDARDRMLRTAAVLFRAQGYHATGLNQVLAESGAPKGSLY
                     FHFPGGKEQLAAESIELAGAELCSAIRAALASTADPATALDHVLALLGDHLVASDFRD
                     GCPVATVALDAGAGSEPIRTACVGAYDSWQSLIADHLRAAGIASADDLATVLLAAIEG
                     ALLLARTRHDLAPLRTVGAHLRLLLALDRSAE"
     gene            12951..13793
                     /locus_tag="VFE39_12350"
     CDS             12951..13793
                     /locus_tag="VFE39_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6259434.1"
                     /translation="MKVHHLNCGTMRPFGGRLVSGTGSLLATAEMVCHCLLVETDHGL
                     VLVDSGLGTGDVTDPDATLTRWWQRMSRPVLDLAETALHQVEQLGFSASDVRHVVLTH
                     LDLDHAGGLRDFPDAQVHVLRTELAAALAGRTGWERTRYPHLQWAHGPRWAPHDPQGE
                     EWFGFQAVRDLPGLPPEILLVPLVGHTVGHTGVAVRESDRWLLHAGDAYFFHGEMRPH
                     APYCTPGLRLMQSRVETARGLRLANQQRLRELVRSHGEEVTVFSAHDPVELRAAQPAG
                     GGIS"
     gene            complement(13786..14316)
                     /locus_tag="VFE39_12355"
     CDS             complement(13786..14316)
                     /locus_tag="VFE39_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6259435.1"
                     /translation="MTEEPFSGPHLVQEAHPEMGTVLGTLWLAVTRAGGAVGFPSDAA
                     EQDVRAVAEQAIEDVRAKRQQLIVIGADHALAGTVFLRRGTGTVVAHRAEVLRLMVRP
                     DLQGRGWGKRLLDAAVAHATALGLEQLLLSTRGGTDMPTFYKRQGWSEVGVFPASLRL
                     GPDDIRDEHWFQLRLS"
     gene            complement(14313..14723)
                     /gene="ndk"
                     /locus_tag="VFE39_12360"
     CDS             complement(14313..14723)
                     /gene="ndk"
                     /locus_tag="VFE39_12360"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET6259436.1"
                     /translation="MTERTLVLVKPDGVERKLVGEVLARIERKGLDFVAVELRTVDRE
                     LAAQHYAEHDGKPFFGSLLEFITSGPVLAAVVEGPRAIAAFRQLAGGTDPVEKATPGT
                     IRGDFALETQFNLVHGSDSPESAAREIKLWFPHL"
     gene            complement(14755..15306)
                     /locus_tag="VFE39_12365"
     CDS             complement(14755..15306)
                     /locus_tag="VFE39_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007028198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HET6259437.1"
                     /translation="MATPTPRRPLLRHTLGRRPLVRILIVAGVVLVAAALLVFEPWAL
                     VIDRRVDEAAPAPSAPTAPQAPAAPIELARGELIAHEHASTGSAVVLQLPDGSRVLRL
                     ENLDTSDGPDLHVWLSDAPVIPGGDGWHVFDDGRHVDLGVLKGNLGSSNYPIPADVDL
                     AQLASVSVWCERFAVSFAAAALS"
     gene            complement(15351..15728)
                     /locus_tag="VFE39_12370"
     CDS             complement(15351..15728)
                     /locus_tag="VFE39_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004562803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4233 domain-containing protein"
                     /protein_id="HET6259438.1"
                     /translation="MTAPDPMKGIRGVFAATLVLESIVVALALLVLSKFGAGATALGV
                     TVIVVLAAAMVVAAGMQRRPWGLGLALALQVVMIACGLLVPVLGVLGVVFALVWGGLL
                     LLRRDVAGRMARGELPSQQLRDS"
     gene            complement(15725..>16195)
                     /locus_tag="VFE39_12375"
     CDS             complement(15725..>16195)
                     /locus_tag="VFE39_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HET6259439.1"
                     /translation="VRAAFAGVTSPGRLERLAAGQGVPTVFVDAAHNPHGARALATAL
                     TTDFRFARLIGVIGVMQEKNARGMLEELEPVLQEVVVTANSSPRAMDPDELGALAVEV
                     FGSDRVSVEPVLAEAVEQAGELAEEGGESGVGVLVTGSVVTAGETRALFGKEPS"
BASE COUNT         2368 a         5874 c         5796 g         2157 t
ORIGIN      
        1 gccctcgatg agcccgggaa cgtcggccac gacgaactcc gtcgtgtcgt cgacccgcac
       61 gacgccgagc gacggctcga gcgtggtgaa cggatagtcg gcgatcttcg gacgggctgc
      121 ggagagcgcc gcgacgagcg aggacttgcc cgccgacggg aacccgacga ggccgacgtc
      181 ggccatcgac cgcagctcga gcaccaggtc gcggttctct cccggctcgc cgagcagcgc
      241 gaacccggga gccttgcgag ccgccgaggc gagcgcggcg ttgcccagcc cgccgcggcc
      301 gccctcggcg gcgacgaagc gggtgccgac cccgacgagg tcggcgatga tctcgccctc
      361 ggtgtcgaac accactgtgc cgtcgggcac ctgcagttcg aggtcctcgg cgttggcgcc
      421 cgcccggaag ccgccctggc cctgcttgcc gttgcgagcc atcgcgtgcg ggcggtggtg
      481 gaagtcgagc agggtgtgca cgccggggtc gacgacgagc acgaccgagc cgccccgtcc
      541 gccgttgccg ccatcgggac cgccgagcgg cttgaacttc tcgcggtgca cggaggcgca
      601 gccgttgccg cccgcccccg cggtggcgtg tagcaccacc cggtccacga acttcgacat
      661 cgacttctcc tcacacacga caacggcggg ccgccccgag ggcggcccgc cgtgttcaga
      721 acttgggtgg tgccgacctc agaccgaggc cggcacgatg ttcacgacct tgcggccgcg
      781 ggtgtaaccg aactcgacgg cgccttggac gagggcgaac aacgtgtcgt cgccgccccg
      841 cccgacaccg gtacccgggt ggaacttggt gccgcgctgg cggacgatga tctcgcccgc
      901 gcccacgacc tggccgccga agcgcttcac gccgaggcgc tgtgcgttgg agtcgcgccc
      961 gttgcgcgag ctggacgcac ccttcttgtg tgccatggct gcgtctccct acttcgcgat
     1021 gccggtgacc tggaccttcg tcaggggctg acggtgcccc tggcgcttgt ggtacccggt
     1081 cttgttcttg aacttgtgga tacggatctt cgggcccttg gtgtgctcga cgaccgtggc
     1141 ggtgactgac gcggcggcca gcgcctccgc atcggtggtg acgtcgtcgc cgtccaccag
     1201 gagcagagcc ggaaggctga cctccgcgcc gggcttaccg tcgatcttct cgacggtgag
     1261 cacgtcatcg acggccacct tgtactgctt accgccggtc ttgacgatcg cgtacatcga
     1321 cgcactgctc ctagctcggg ggacgaagca cagaactctg tcctcttctt agctctgcgc
     1381 gggccgcatc agccgatgaa cggagtgcgg cgcaggactc ccagggtacg gaagggtttc
     1441 ggggagggtc acaccgggtc tcccccgctc tccccggtgc cgtcggtgac gggcgggccg
     1501 gccgggcggg atgccgcgcg gcgggtgcgc cggggccgcg tggccccggt cggggcgggc
     1561 tgcggcgggg ccgcgacggg cgttgcgggc tcgctcgagg ccgtgcgtgc agccgggacc
     1621 gtgaccacgg ccgcgaccgg agcgtccccg gcgaccggag cgggcgcgcc cgccgtgcgg
     1681 gtgacgcggc cacgccgctg gcggggccgc ggctgcgcgt gctgctcgga gtcggcagca
     1741 gcggagcctg cctcgggaga ggccaccacc ggagagtcgg ccacaggagg gcctgccact
     1801 gcggagccgg ccaccggaga gtcggccaca gcggagtcgg ccacagcggc ggcggtcggt
     1861 gaggcggcgg ccggtgcggc ctgctccgcg atcgaccgct cgacgacgac ctcctcgacc
     1921 gcgtcggcga cgacgtcgac cggacgcgcc tgcggggacg tctcgacgac gccccgacgc
     1981 gcgatcctgc gaccaccggt gcggcgaccg gtgggaacgg gcacctcgac gaccgccggc
     2041 tcgaccgcgg gagcgtcgat gaccggcgca tcgggagcct cggcgaccgg gacgtcggcc
     2101 acggcggggg cctccacgac cacggggtac gcagcctcga ccaccggcgc cggagcgccg
     2161 accccgacct cggcatcgga cgtgttggtg tcgacccgca caccgttctg ctggccaccg
     2221 ttctgctggc caccgttgta ctggccgccg ctgtgctggt cgccgtggcc gttgcgctcc
     2281 tcgctgtcac cgcgccgacc ccggctgcgc gaccggcggc cgggcgcgtc ccggtcggag
     2341 cggtcgccgc ggtccgagcg atcgtgccca gagtcgccct tctcgagggt ctgctcggcg
     2401 ttgacgatca cgccccgccc gcggcagtgc tcgcacggcg tggagaagtg ctcgaggagc
     2461 cctccgccca cgcgcttgcg ggtcatctgc acgaggccga gcgaggtgac ctccgccacc
     2521 tggtggcggg tccggtcgcg ggccaggcac tcggtgaggc gccgcagcac gaggtcgcgg
     2581 ttggcctcga gcaccatgtc gatgaagtcg atgacgatga tgccgccgat gtcgcgcagc
     2641 cggagctggc gcacgacctc ctcggccgcc tccaggttgt tgcgggtgac cgtctcctcg
     2701 aggttgccgc cggacccggt gaacttgccg gtgttgacgt cgatgaccgt catcgcctct
     2761 gtgcggtcga tcaccagcgt gccgccggag ggcagccaga ccttgcggtc cagccccttg
     2821 agcagctgct cgtcgatccg gtactcggcg aacacgtccg atgggccgac atggcggtgc
     2881 aaccggtcgg cgagctcggg agcgacgtgc cccacgtagt tctgcaatgt gtcccaggcg
     2941 tcgtcgccct cggcgatgag ccgggtgaag tcctcgttga actggtctcg gacgaccttg
     3001 aggagcaggt cgggctcctc gtagagcagc accggggcct tgggcttgtt cgggcccgtc
     3061 tgctcggcct tggtcttgac gacctcccac tgcgcctgca ggcgccggac gtcgcgctcc
     3121 agcgcctcgt ggctgacgcc ctccgaggcc gtgcggatga tcactccggc ctcgctgggg
     3181 acgatctcct tgagcatgtc cttgagccgc ttgcgctcga cgtcgggcag cttgcggctg
     3241 atcccggcgg cgccgccgga cggcacgtag acgaggaagc ggcccgccag gctgatctgc
     3301 gtggtgagcc gggcaccctt gtgcccgatg gggtccttgg tgacctgcac gagcacgctg
     3361 tcgccgctgg agagcgcctg ctcgatgcga cgggccttgc cgcccagacc ggcggcgtcc
     3421 cagtcgacct cgccggcgta cagcacggcg ttgcggccgc ggccgatgtc gacgaacgcg
     3481 gcctccatgc tcggcagcac gttctggacc cggccgaggt agatgttgcc gaccatggac
     3541 ggcccggtgc cgccgtggtc ggtgacgaag tgctcgacga gtacgtcgtc ctcgagcacg
     3601 gcgatctcga cgcggtcgcc ccgctcgcgc acgaccatcg agcggtcgac ggactcacga
     3661 cgggccagga actcggcctc ggacaggatc gggacgcgac ggcggcccgc gtcccgaccg
     3721 tcgcgacggc gctgacgctt ggcctcgagc cgggtggagc cgcggacgct ctgcacgccg
     3781 tcgccgcccc cgtcggcgct gtcgtccgaa cgctgctcac ggggctgacg cacccgcacg
     3841 acggtgttcg gcgggtcggg ctcgtcggcc gtgtcggcgg tatcggcgcc gtcgtcagcc
     3901 gatccacgcc ggcggcgacg ccgacggcga cggcgcgagc cgccggcctc gtcgtcgctc
     3961 tcgcggtcgc tgtcctcgtc ctggtcggaa cccgactcgg ccccggcgcc cgcggcgccg
     4021 gccgcggtgg tgtcgacgga gacggcctcg tcggagtcgt cggagtcgtc ggagtcgtcg
     4081 ccgctctcgt cgtcgaaacc gtcgctgccg cggccccgac cccggccccg gcggccacgg
     4141 cgcctgcgcc tgcgggcacc gtcgtcggag tcgtcggagt cgtcgccgtc ggtggcggcg
     4201 ggagcgggct cggcgacgcg ctcctctaca ggagcgtcgg tactgacagg agacagctcg
     4261 gtctcatcgg agacgggggt cgcctccggc tcggctccac ggcggcgagt gcgtgcaggc
     4321 cgggtgggcg gctggaacag cggggtgggg ggtgcgaaca ccggagcgag cggggcggcc
     4381 tcctcgacgg gggcctcctc gaccgcggcc tcggcaggct cggcggcggg ctcggggtcg
     4441 gccgaccgag ccgcggtggc cggcacgagc gcggtgacga cctgctcggc caccttgcgg
     4501 tcgatgctgg actgcgcgct gcgccccgcc tcgcccaggt cggccagcac ggcgaggacc
     4561 tcgcggctgg tgcgcccgag caccttggcc agcgcatgca cccgcatctt cgcgggcagg
     4621 tcgtccagga cgggtacggt gaccggcccg ccggtggtac cggcgggcga atcgttattc
     4681 gacattcagc tcctccgccc ccgggcgcct ccggagggag gcggccgcgc agggacacgt
     4741 gcagttcttt ctctccgcgc ggtgcgcgcg acgagcaatc ctttggtctc gctccgcccc
     4801 ggggggcggc tcgttgctcg acggcgtgcc gtgaccggca ggggcgccgg tgcgccggga
     4861 gccgacccgc ccgttcaggc gcgggtggtg acccggtcgg aaccgagtgg gtcggccaag
     4921 tcgcctcgat ggtcgagcag gccctgcgcc atccgggtgg ccttcgcggg caccggcgac
     4981 ttgaggcctg cgacaacgtc gagtgcaccc aacacgtcat cgggtcgaac ggcgggtgtc
     5041 gtgtgccgta caaccgccgt cattatcgca cagactgccc cctgatcggg gggtgtcgac
     5101 agtgagccac ccgacgtagc ccccggagcg ttggcgtccc ggctcacctc ggcgtgcacg
     5161 agcgccgccc tgacgtcgat ctggcgccgt ccggacggcg tgacgcgttc gacgacgatg
     5221 gactcctcgg ccagcagcgc cgcgagcgcc gcccgcaggg cgtccggcgc caccccgggc
     5281 agctcgatca cccagcggct gccgtcgatc cgttcggcca gcgcggtacc gcaggccacc
     5341 gcggcgtcga gcacgtcgag cccgtcgggc agtgccgcgt cgagcgcggt gacgagcgcc
     5401 gccggatcga cctccctggt cagaccgatc tccaggtact ccgcctcgct ggccgagccg
     5461 gtgggcgccg cgccgatcca ggacagccgc gggtgcgggc tgaacccgtg ggagtgggcc
     5521 accggcaccc ctgcccgccg cacggcgcgc tcgaagctgc gcgccacgtc ccggtgggag
     5581 aggaagcgca gcctgccccg tttggcgaag cgcagccgga tgcgctgcac ggtgggaggg
     5641 gcggggttcg ccgcctcccc gggacgcacg cgggccatgg agccagccta cgagcaccgg
     5701 cctggcggcc accccgtggg cagcccggga gcacggtccg gacgacggca gggaccgcgc
     5761 gcggcccacc ccgcgaaacg agcggcgcgc ggcccgcaac gtcagagcac gtcctccagt
     5821 cggatgggca ggcgccggaa ccggcgtccc gttgcgtggt ggaccgcgtt ggcgatggcg
     5881 cctgcgacgc cgaccatgga gacctcgccc agccccttgg tgggcgagct cgtcgtccgc
     5941 gagaacctcc acctcgtcgt ccggggcctc gaggtgctgg ccgaactggg tggcggagcg
     6001 cacggggccg cgacccgcgg gacacggcag tgcgcgcgtt caccggggcc gggctcggcg
     6061 tgagcatgcg cgtgctcctc gacgcggcgc aggagcctgg gggcgatccc gcgcaggcgc
     6121 tcgacgaggc gctcgccctc ctcgatgccg ggctaccgct ctgaccgtcc tactgcggcg
     6181 gcgtcgggag ggtggacggg tcgagcatca gcatctgttc ccccgcgggg acgaagcccg
     6241 cccgccggta gaacgcggta cttcccccgg tgggagccag cagcagccgt cggtagcggc
     6301 gatcacgggc gtgctccagc gccgcggcca gcagggcggc aggcacgccc ccgtcggcga
     6361 acgacgcgag cacgaaggcg ttgcccacgt gcccgatccg gccgctgcgc gcacccgggc
     6421 ggggcatgtg cacgatctcc accacgttga tcgaaccgac cgcggtgaag ccgctgcgcg
     6481 ccgacccgac ctcggccagc cagaacgtcc gccgcggcag ctcgacgcgc caccactgtt
     6541 cgaacgcggc ctcgaaaccc gggtcctcga ccggccgccc cgtgcgctcc tcgagccatg
     6601 tgcggcgcag ccgcgccagt gcggctacgt cgcgctcgtc ggcgacacgt attccgacct
     6661 gcggaccgga accggcgcga tcagacactc gtgcccgggc ccccggacac ggggttcacg
     6721 acggtcagcg ggagcaaggt ggtgccgctg ggcccgacct cgatgtccgt gccggtggaa
     6781 gggcagacac cgcagtcgaa gcacggcgtc cagcggcagt cgtcctgctc ccggccggag
     6841 agggcgtcct gccagtcgtc ccagagccag tcccgctcca ggccggagtc gaggtggtcc
     6901 cacggcagca cctcgtcgcg accgcgctca cgcgtggtga accagtcgag cgagacgccc
     6961 agcggctcca gctcggccgc ggcggcaccc gtccagcgct cgtaggagaa gtgctcgctc
     7021 cagccgtcga accgcccacc gtcgcgccac acccgctcga tcacgcggcc gacgcgccgg
     7081 tcaccgcgcg cgagcaggcc ttcgatgagc gagggctgcc cgtcgtgata gcgcatcccg
     7141 acgttgcggc ccagcttccg attgctgttg accgccgccc gcagcttgcg cagccgctcg
     7201 tcgacgacct cggggtgcgc ctgggcggcc cactggaacg gggtgtgcgg cttcggcacg
     7261 aagccgccga tcgagatggt gcagcggatg tcgttgtgcc cggacgccgc gcggcccgcc
     7321 tggatcacgc ggtgcgccat tccggcgatc tccaggacgt cctcgtcctc ctcggtgggc
     7381 agcccgcaca tgaagtacag cttcacctgc cgccagccct gcgcgaacgc ctcgctgacg
     7441 gtgcggatca ggtcctcctc cgacaccatc ttgttgatca cgcgccggat ccgctcggac
     7501 ccgccctccg gcgcgaatgt cagcccggag cggcggccgt tgcgggagat ctcgttggcg
     7561 aggtcgatgt tgaacgcatc gacgcgcgtg ctcggcagcg acaacgacgt gttcgtgccc
     7621 tcataccggt cggcaaggcc cttcgtgatc tccgcgatct ccgagtggtc ggcgcttgac
     7681 aacgacagca gcccgacctc gtcgaacccg ctcgcgcgca ctgccgagtc gaccatctcc
     7741 ccgatccctg ccaaggtccg ctcgcgaacc ggccgggtga tcattcccgc ctggcagaaa
     7801 cggcagccgc gggtgcaccc gcggaagatt tcaacgctgg cccgctcgtg caccgtctcg
     7861 gccatcggca ccaacggctt cttcgggtag ggccatgcgt cgaggtcggt ggtggtgcgc
     7921 ttggtgacgg agcgcggcac gcgctcgtcc accggcgcga ccgacgcgat ctcgccgtcg
     7981 tcgccgtagg acacggcata gagcgacggg acgtagcagc cgtcggtggc ggcgaggcgc
     8041 agcaacagct cgcgcctgcc ccctggcctg ccggccgcct tccactcctt gacgacgccg
     8101 gtgatctctc cgaccacctc ctcgccgtcg ccgatcgcgg caacgtcgac gaagtcggct
     8161 attggttccg ggttgaacgc ggcgtggccg ccggccacca cgaccggatg gtcgatcccg
     8221 cggtcgatcg cgtgcagcgg gataccggcg aggtcgagcg cggtgaggag ggtggtgtag
     8281 ccgagctcgg tggagaagct cacgccgagc aggtcgaagg ccccgagcgg ccggtgcccg
     8341 tcgacggtga acgccggcac accgtgctcg cgcatcagcg cctcgagatc gggccagacc
     8401 gcgtagcagc gctccgcgag ggcatcgacg cgctcgttga gcacctcgta gaggatcatg
     8461 aggccctggt tgggcagccc gacctcgtac gcatcggggt acatcagcgc ccaccggaca
     8521 gcggccgact cccagtcctt gagctgcgcg ttcagctcgc cgccgacgta ctgcacgggc
     8581 ttcgagacgc gcggcagcag cggttcgagg tcgggataca ccgacggaac tgtcatgatg
     8641 ccgccgctcc cgctgggaga atgcacgtcg ccggtcacgc tgatccaggg tagccccacc
     8701 gcctgagggc tacgcgaccg tgcgcgaccc ctggcggatc gtgcgcccgg atactcgccg
     8761 cggcgaccct gacgcctatc cccaggcggc gcgcacgggg ttgcccgcag tggtgagcct
     8821 gcgcgtggcc gcagcgatca cggtgacgat cgcgatcgcg ccggcgacga ccactccggc
     8881 gggcgggtcg ttcagcgcga tgccggcagc cgcccacacc gccgcgatgg cgtaaccgac
     8941 gacagccgtg cccgagagca ccacccacga cgcgatgacc gacaccgcca gcagcacgac
     9001 cactgcagcg atcgcagcca gagcaccggt gccgggcaat ccgacccata ccccggtggc
     9061 cgcggtacag agggcgacgg cgaacgacac ccatcccgtg tacaacgcca ccgtgccacg
     9121 cagcacgacc cgctccagga cgctgtcggc cggctgccgg ctgagccgac cgaacacgac
     9181 cgcgagggtg acgagcagcg cgaggatcag cagctcggcg aggggcagcc agcctgcctc
     9241 gaacgccagc acccaggcga tgttgaacac ggccgacgcg gcgagccacc atccggtctc
     9301 gcggtggatc tggcgccccc gctgagccgg gagcacctgg tagacggcat aagcgaggaa
     9361 accggcatag atcggcgccc agatcgcgaa cgcccagccc gccgccagca gggggctggc
     9421 gtactcatgg gccaccgccc cggccgtatc gcccgcgaga ccgatcccgc cgagaccgga
     9481 cacgacaagc tggagcactg cgagcagcac cacaacggcg ttccggatca ggtcggcgag
     9541 caccggtcgc tggaacgtta cggtcatgcc ctgcattgtc gacctgctta cggcccgcgg
     9601 ccagcccagt cgccgacggg tccggacgcc ggggtgagcg gaagatcgac cggtctcacc
     9661 ggagtagtag cacgtccagc cgtcgcgatg ccaccatgac gccgaccacg atcatcacca
     9721 ggaagtacgc gaggtggccg accatcccga ggtgcaccgc acccgtggtg aggccacgca
     9781 tcaactccac ggcgtggaac agcgggagga actccaccac gagctgcagc caccgcgggt
     9841 acaccgcgag cgggtagaac gtggtggaga acaggaacat cggcaggatc gcgagcgtga
     9901 caaggtcgaa gtcctgccac gaccgcatgt aggacgtggc ggccataccg acggccgcga
     9961 acgcgaacgc cacgaccaac gcggcgggca gtgcgagcag cgcccacggc gagacgagca
    10021 ggccgaagcc ggccatcacg gacaggaacc ccaacgcgta gaggccaccg cggatgagcg
    10081 cccagccgat ctccccgagc gcgatgtcga ccggcccgag aggcgtggcc agcatggcgt
    10141 cgtagagcct gccgtacttg agcttgaaga agacgttgaa ggtggagtcg aacacggccc
    10201 cgttcatcgc cgacgccgcc agcagccctg gcgcgatgaa cgcggcgtag ctgatgggtg
    10261 cgccgtcggg acccgggagt acaccgacca gcgacccgag cccctggccc atcgcaacca
    10321 ggtagaagac cggctcgaag aaccccgaca cgaacgcgag ccaggcccgc cgggacgcgg
    10381 tggccgagcg gagcagcagc gcgcggctgc gcccggcgta gcttccggcc gggatcagcc
    10441 gcagcagcgc gggaggggcg ggtggcgcgg gcacctgcgc ggtcactggg ccagcctcct
    10501 ggcgaagaaa cgggtggcga gcgcgagccc gagcacgagc agggcgcaca ggtaggccag
    10561 gtgccccagc gcggcgagcg gctgtggcac ggcaccgagc gccgcggagc gggccagctc
    10621 ggtgccgtgc cacagcgggg agatcaacgc aaccggctgc acccaccccg gtaaccggtc
    10681 gaccgggaag aacgtgcccg agaacagcgt catcgggatg acgacgaagc ggaagagggc
    10741 gttgaacgcc gcgccctcgt tctccacggt ggcggagaac gccgttacca gggccgccac
    10801 agcggctccg gtgagcacgg ccaccatcaa cgacaccacg atgccggggc cggtgacacc
    10861 cccgaagacg gcgatcactc cgatgtagat ggcgccggca ccggccatct tgcacacgac
    10921 ccagagcagg tgtccgagcg cgacctgcgc cggggagatc gggcccgccg tcatcgcgaa
    10981 gtaggtgcgc tgccacttga atcccgacag caccggatac gtggactcga aggccgcgct
    11041 ctgcaccgtg gacacggcca gcagcgcggg cgcgagccac acgatgtagt cgacaccgcc
    11101 ggtggccgcg gcggcgctgc cggtgccggc gaccagcgat ccgaagccga ccccgaaagc
    11161 caacaggaac aggagcggtt gcagcaccga cgacaccgcg gtggcccgcc agttgcgccg
    11221 gtaccaggtc cagaagtgct cgaccaccaa cagcaccccg cgccaccgcc cgggcgcccg
    11281 accggtggag cgtgcgagct ccctcagtgc tgatgattcg ttcgcactcg ccctcagtgc
    11341 tgatggttcg ttcgcacgct ctctcatcag tccaccaggg ttcggccggt gagccggagg
    11401 aacacgtcct ccagcgagga gcgccgcacc agcgccgaga gcggcacgaa gccggcgcgc
    11461 gcgacctcgg cgtgggtgtg ctcgccgtcg gccgtgtaga gcagcagccg gtcgggcagt
    11521 tcctccacgc gctcggccaa cagcgcgagg gcatcgacgc cggggcggag ggcggggtcg
    11581 aaccgcagct ccagcacctc tcgcgtggag taccgctcga tcaggtcggc cggcgacccc
    11641 tccgccacga tcacgccgcc atccatcacg acgagtcggt cgcacagctg ctcggcctcg
    11701 tccatgtagt gggtggtgat gatctgggtg acgccctcgc gtttgagccg gtacagccgc
    11761 tcccacagca ggtggcgggc ctgcgggtcg agccccgtgg tgggctcgtc gagcagcagc
    11821 agctcgggct cgttgaccag ggcgcgcgcg atcgtgagac ggcgcttcat gccgccggag
    11881 agtggctcga ccgaatcgtc ggcgcgctcg gtgagctgtg cgaactccag cagctcggtg
    11941 gccttggccc gcacgtgcgc gcgggagagc ccgaagtagc ggccgtagat ctgcaggttc
    12001 tgccgcaccg tgagctcggt gtcgaggttg tccagctgtg ggacgacgcc gatgcgggcc
    12061 cggatgcgcg ggccgtcgac gtccgggtcc atgccgagca cctgcagcgc accgtcgcta
    12121 cggggcgaca cgcacgcgat catccgcatc gtggacgact tgccggcgcc gttaggcccc
    12181 aggaagccga acacctcgcc gcgtgcgaca tcgacgtcga ccccacggac cgcctcgaag
    12241 tcgccgaagc gcttgcgcag cccgcgggcc tgaaccagtc catccgccac gaacagcacg
    12301 ctagcggcga gccccgacat agcgcgtgga cgtttcagac ttggttctgg ctagaccggt
    12361 ctatatagtt cggtcatggc agttcgaacc gacgcccgcg accggatgct gcgcaccgcg
    12421 gcagtgctgt tccgggcgca gggctatcac gcgacaggtc tcaaccaggt gctcgccgag
    12481 agcggcgccc cgaagggctc gctgtacttc catttccccg gcgggaagga acagcttgcc
    12541 gccgagtcga tcgagctggc gggcgccgag ctgtgcagcg cgatcagggc cgcgctggcg
    12601 agcactgcgg acccggccac agcactggac cacgtcctcg cactgctcgg cgaccacctc
    12661 gtcgcgtcgg actttcgcga cggctgcccg gtcgcgacag tcgcgctgga cgccggagcc
    12721 ggcagcgagc ccatccgcac ggcctgcgtc ggcgcctacg actcgtggca gtcactcatc
    12781 gcggaccacc tgcgggctgc ggggatcgct tccgccgatg acctcgccac cgtgctgctc
    12841 gccgcgatcg agggcgccct gctgctcgcg cgcacccggc acgacctcgc tccgctgcgc
    12901 accgtcggcg cgcacctgcg cctcctgctg gctctcgatc ggagtgcgga atgaaggttc
    12961 accacctcaa ctgcggcacc atgcgcccct tcggcggcag gctggtgagc gggacgggat
    13021 ccctcctcgc caccgccgag atggtctgcc actgcctgct ggtcgagacc gaccacggcc
    13081 tcgtcctggt cgacagcggg ctcgggacgg gcgacgtcac cgacccggac gccaccctga
    13141 ccagatggtg gcagcggatg tcccgcccgg tgctggacct ggccgagacc gcgctgcacc
    13201 aggtcgagca gctgggcttc agcgcgtccg acgtgcgcca cgtcgtgctg acccacctcg
    13261 acctcgacca cgccggcggt ctgcgcgact tccccgacgc ccaggtgcac gtgctgcgca
    13321 ccgagctggc ggcggcgctc gcggggcgca cgggctggga gcgcacgcgc tacccacacc
    13381 tccagtgggc gcacgggccg cggtgggccc cgcacgaccc gcagggcgag gagtggttcg
    13441 gcttccaggc ggtgcgcgac ctccccgggc tgccgcccga gatcctgctg gtcccgctgg
    13501 tcggccacac cgtcgggcac accggcgtgg cggtgcgcga gagcgatcgc tggctgctgc
    13561 acgccggcga cgcgtacttc ttccacgggg agatgcgccc ccacgcgccc tactgcacgc
    13621 cgggactgcg cctgatgcag agccgggtgg agaccgcccg cgggctgcgg ctcgccaacc
    13681 agcagaggct gcgtgagctc gtgcgctcgc acggggagga ggtcaccgtg ttcagcgcac
    13741 acgacccggt ggagctgcgg gccgcgcagc ccgccggcgg cgggatcagc tgaggcgcag
    13801 ctggaaccag tgctcgtcgc gtatgtcgtc ggggcccagc cgtagcgacg cgggaaagac
    13861 gcccacctcg gaccagccct gccgcttgta gaacgtgggc atgtcggtgc ccccgcgggt
    13921 cgagagcagc agctgctcca gcccgagcgc ggtagcgtgg gcgacggccg cgtcgagcag
    13981 cctcttcccc cagccgcggc cctgcaggtc tgggcggacc atgagccgga gcacctcggc
    14041 gcggtgcgcg accaccgtgc ccgtgccgcg gcgcaggaac accgtgccgg caagcgcgtg
    14101 gtcggcgccg atcacgatca gctgctgccg cttcgcccgc acgtcctcga tcgcctgctc
    14161 ggcgacggct cgcacgtcct gctcagcggc gtcggagggg aacccgaccg caccaccggc
    14221 ccgcgtgacc gcgagccaga gcgtgcccag caccgtgccc atctccggat gcgcctcctg
    14281 caccaggtgt gggccgctga agggctcctc cgtcacaggt gggggaacca gagcttgatc
    14341 tcccgtgcgg cgctctcggg cgagtcggat ccgtgtacga ggttgaactg ggtctccagg
    14401 gcgaagtcgc cccggatcgt gccgggggtg gccttctcca ccgggtcggt tccgcccgcg
    14461 agctgccgga acgcggcaat cgcccggggg ccctcgacta cggcggccag taccggaccg
    14521 gaggtgatga actccagcag gctgccgaag aacggcttgc cgtcgtgctc ggcgtagtgc
    14581 tgggcggcga gctcgcggtc gacggtgcgc agctcgacgg cgacgaagtc gaggcccttg
    14641 cgctcgatcc gggcgaggac ctcgccgacc agcttgcgct cgacgccatc gggcttcacg
    14701 aggaccaggg tgcgttcagt cacgtgcgca ccctatcgac ggcctctgcg ccgctcagga
    14761 gagggcggcc gccgcgaacg acacggcgaa acgctcgcac cagacgctca cgctggcgag
    14821 ctgcgcgaga tcgacgtcgg ccgggatggg gtagttcgag ctgccgaggt tgcccttgag
    14881 cacgccgagg tcgacgtggc ggccgtcgtc gaagacgtgc cagccgtctc cgcccgggat
    14941 gaccggggcg tcggagagcc acacgtggag gtccggaccg tcgctcgtgt cgaggttctc
    15001 cagccgcagc acccgggacc cgtccgggag ctgcaggacg acagcggatc cggtgctggc
    15061 gtgctcgtgg gcaatcagct cgccgcgggc gagctcgatc ggtgcggcgg gtgcctgggg
    15121 ggccgtcggg gcggacggcg cgggggcagc ctcgtccacc cgccggtcga tgacgagtgc
    15181 ccagggctcg aagacgagca gcgcggcggc gacgagcacg acgccggcga cgatcaggat
    15241 gcggacgagg gggcggcggc ccagcgtgtg tcgaagtagt gggcggcgcg gcgtgggagt
    15301 tgccatacag gtcagtgccg caccaccgcg cgtccgttac gccgctcttc tcacgaatcg
    15361 cggagctgct ggctgggcag ctcgccgcgc gccatcctcc cggcgacgtc gcggcgcagc
    15421 agcagcaacc cgccccagac gagtgcgaac accacgccca ggacgcccag cacgggtacc
    15481 agcaggccgc aggcgatcat cacgacctgc agcgccagcg ccagcccgag cccccacggc
    15541 cgccgctgca tcccggcggc gacgaccatc gcggcggcca ggacgacgat caccgtgaca
    15601 cccaacgcag ttgcgcccgc gccgaacttc gacagcacca gcagcgccag cgcgacgacg
    15661 atcgactcca ggacgagggt ggcggcgaat acgccccgta tccccttcat gggatccggc
    15721 gcggtcatga cggttccttg ccgaacaggg ctcgcgtctc ccctgctgtg accaccgagc
    15781 ccgtgacgag tacaccgacc cccgactcgc cgccctcctc tgccagctcc ccggcctgct
    15841 cgacggcctc ggcgagcacc ggttcgacgc tcacgcgatc cgagccgaac acctcgacgg
    15901 cgagcgcacc gagctcgtcc ggatccatcg cgcgcggcga ggagttggcc gtgacgacca
    15961 cctcctgcag caccggttcc agctcctcca gcatgccccg ggcgttcttc tcctgcatga
    16021 cgccgatgac gccgatcagg cgggcgaacc ggaagtcggt ggtgagcgcg gtggcgagcg
    16081 cgcgggcgcc gtgcgggttg tgcgcggcgt cgacgaacac cgtcggtacg ccctgacctg
    16141 cggccagccg ctcgagcctg cccggcgagg tgacgcccgc gaacgcggcc cggac
//
LOCUS       DASRSC010000252         9242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3210398,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000252 DASRSC010000000
VERSION     DASRSC010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3210398"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(559..726)
                     /locus_tag="VFE39_12380"
                     /pseudo
     CDS             complement(559..726)
                     /locus_tag="VFE39_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034719.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fpg/Nei family DNA glycosylase"
     gene            complement(729..1220)
                     /locus_tag="VFE39_12385"
     CDS             complement(729..1220)
                     /locus_tag="VFE39_12385"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HET6259440.1"
                     /translation="MRVYLGSDHAGFELKAHLVAHLTGLGHEAIDVGPVSYDPEDDYP
                     PFCIEAARRTIADPGTLGVVIGGSGNGEQIAANKVPGIRAALAWSIETATLSRQHNDA
                     QVVAVGARMHSLDEAAEFVSTFVATPFSGEPRHQRRIDLLAEYERTGTPPPLPPGQVP
                     AAS"
     gene            complement(1289..2626)
                     /locus_tag="VFE39_12390"
     CDS             complement(1289..2626)
                     /locus_tag="VFE39_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET6259441.1"
                     /translation="MTTTERPRRGRGTAIGEGITWAARWSLRLLLVAAGAWLVWTVIA
                     LLWSVVFPVIMAVIVSTVLWPPTAWLRRHGFPPALASATVLLAGMVVLAGLIALITTS
                     VAGSIPEISRAASSGIQSIQQWLAGRPLNFAQGQLDAVLQNATTQLQQSISVITTSVL
                     TGIGSVASGVITALLTLVLVFLFLKDGPRFLPWLRRVMGEGAGGHLAEVLRRVWKTLA
                     DFIRVQAIVALVDSVLIGLGLVILGVPLAVPLAVLTFLGAFIPIVGAIVAGALGVLVA
                     LVSNGFTTALIVLAIIVAVQQLEGNVLQPVLQGRSLGLHAAVVLLAVTAGSTLFGIAG
                     AFLSVPVAAAGAVVLRYLSERVDARASGEAQPPRVDPPLETVQTVLAMPGGLLPDGSA
                     PDPDAPEPAGPATHTEDVAPEDEDEGQGEDRARADRAEESTDGRTEDKGTGRR"
     gene            2761..3621
                     /locus_tag="VFE39_12395"
     CDS             2761..3621
                     /locus_tag="VFE39_12395"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HET6259442.1"
                     /translation="MSTDSDGPAAGIVLAGGRSSRMGESKAALEWHGSTLLRRTAGLL
                     ARAVDGPVVVVRAPGQPLPALPAGVEAVDDPVEGLGPMQGIATGLAAVADRAQVAFVC
                     STDLPFLHPAYVRRVLAALDADVDVVLPHAHGHRQPLAAAYRTALAPVVAGLLADGQL
                     KPAMLFDNCRVHRTDDADLLADPRLGALDPGLDSVLNVNEPDDYRAARARPAPEITVQ
                     RYGALASNGRRGPESVRAATLGAAAAAVGLALDRHVVAALNGDQITRDAEMPLVAGDM
                     VAFLSADAGG"
     gene            3662..5458
                     /locus_tag="VFE39_12400"
     CDS             3662..5458
                     /locus_tag="VFE39_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HET6259443.1"
                     /translation="MTAGGYFGRALVVDVRGDSATGTPLELDDRVLRAYLGGVGLGTW
                     LLHRLAPPGVDPLAPEAPLAFVFSPLVGTPLTTSAKFAVLAKSPLTGMITDALASSQF
                     AISGKLTGHDAIVVRGRADELSVVMVDASGVRLEAAPELAGMAAAEAESAVRERFGRG
                     WRTAAVGPAAERGVRYATISHDGRHAGRGGLGAVLGAKNVKAVLVKAGTRVDVADQQA
                     VLAAAKDLRERSFGPATAKYRELGTLANLLAFNAVSTLPTRNFTAATFAEAPRLAAEE
                     LHEMRGVARNSCASCSIGCEHIYSRKGGGSQRMEYENVFALGPLCGVSDPDDVFAASA
                     RCDELGIDTISAGGTIAWAMECVERGLIDAPWLRFGDGAALLRALDLIGSRTEGLGAL
                     LAMGSRAAAAVVGQGSIDFAPQVKGLELPGYEPRSLQSMALGLAVNARGADHNRSGAY
                     EADLSGDLDRLAGGSAHVAAAIGTEDRAAVMDSMILCKFLRGVFTEPFDEWAALLRAV
                     TGWDVTADELRTAARRIVLAKRLFNLREGATTDDDTLPRRMLDTPLELGSGRTATLTA
                     DRLRSMVEGYYTGRGLDVEGRPDPADLADLLL"
     gene            complement(5462..6967)
                     /locus_tag="VFE39_12405"
     CDS             complement(5462..6967)
                     /locus_tag="VFE39_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET6259444.1"
                     /translation="MTEVQRSASTYPLGVSRPDFRMDDLPTPEEVFRVRRIGPRQMVM
                     FVIGPSLIALGISIGSGEWLLGPQAVGQYGFVGVGWVIVVSAVLQTFYNVECARYVVA
                     TGEAPVVGWGRVPPGKMLWIPLSVFVVIFAFIAGGWAASAGQGLFALVNGRVADASEV
                     QQTRWYAIGLLVVVFLITASARRISRALELSNWVMVGFILVTLIVVDLIVVPWSVWWE
                     GIRGFVTPAAPPAGITATQLGALAGFTALASGLNWYVMGHYRDKGYGMGHRTGYIAGM
                     RGGQRELLSVGVTFPDDERNTSLWRRWYRLLMVDMWGVFFVGAMLGMLLPTVLMSQAV
                     ALSGEQPTAANVPTFAASALQTEYGSGMFYLLLVLGVLILFSTQLGIFEAMVRVVTDA
                     APAVSPRMRALIEGDPRRFYYPFMIALLVAISVILHLAVPVTLVQFSANMSNLGALIY
                     PFLLIYLNSRLPKAARPRPWHYVILLLNFLFFGFFFVNFVADLLGDPLITF"
     gene            7252..9117
                     /locus_tag="VFE39_12410"
     CDS             7252..9117
                     /locus_tag="VFE39_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6259445.1"
                     /translation="MYHAANAQEEKRPFSMATLRRIVAFVGPHRRRMATFLLVSVIGA
                     VLAVATPVLAGRVVDAIVKGGPMGVVIGLAGLIAVIAVAVAVSGIVQRWLSASLGEGL
                     ILDLRAAVFHHVQRQPIAFFTRTRTGALVSRLNNDVIGAQRAFSDTLSGVIGNLVTLT
                     LTLVVMLTLSWQVTLLALVLLPVFVLPARRMGHRLAYLEREAADHNAAMGTQMTERFS
                     APGATLIKLFGRPAEETREFVARAARVRDIGVRTAMVQWVFVTALMLVSALALALVYG
                     LGGYIALTGGMAAGDVVALALLLTGLYAPLTALASARVEVMSALVSFERVFEVLDLPP
                     LIAEPADPLPLPEGPLSVEFDGVRFGYPAADKVSLASLEEVALLDGRGGEEVLHDVSF
                     TAESGQMIALVGSSGAGKSTIAQLLPRLYDVDAGAVRLGGVDVRRLSFDTIRGALGLV
                     TQDGHLFHESIRSNLLLARPEAHDDELWEVLRRARLADLVASLPDGLATVVGERGYRL
                     SGGERQRLTIARLLLARPRVVVLDEATAHLDSTSEAAVQEALGEALLGRTAIVIAHRL
                     STIRAADQILVLEAGQIVERGTHAELIAQDGRYAELHRTQFAQPEVAAPVALAGI"
BASE COUNT         1372 a         3363 c         3222 g         1285 t
ORIGIN      
        1 ccccgacgat ctcgccgtgg aagtcgggat tctggaaccc cagcacaatg agctggacag
       61 gtccgatagc catgtctctc cctttctccg tcagcagccg cagttcgcgg gagtcgttgc
      121 cgacgtcgcc cgcggaccag ggtcctcagc gtcaccgccc gtgcctcgga tcgcctcatc
      181 caccacgggt gacagccggc tccgtcgagc gcccacgacg agctcagccc gcaactgctg
      241 ggggaggcca cgtcgagacc cagggaactg cacagcgcca acagctcgcg caggggcgac
      301 tcgtcgggat ctcaccgtag atgatggtct ccgggggcga ccgggcggac cttcatctcc
      361 tagaacgcgt tgcacgctcg ctcggacatc cgttcggtca cccggaactc atagcgtgcg
      421 cccgccaaca ccgaccaatc caccgacccg gggcctgcgc gatggacgcg cactccgaag
      481 cggaagccgg actaggaccg gacttggtca gcttcacaag cctcgcacca gcaactatgc
      541 acctctgcac ccgtcctagt ggaacaggtg cttgccgtgc gcctcggcac gcaccagcac
      601 tcggccgtcg acggccgcgg cgctgccggc gacccggccc tgcgggctcg acacggccac
      661 gggtgcgccg gcgaaccggc ggcggtgcag ccgggcgagc cggtggaggg tgtggccttc
      721 cggcatcgct aggaggcggc cgggacctgg ccgggcggca acgggggcgg ggtgccggtg
      781 cgttcgtact cggcgagcag gtcgatgcgc cgctggtggc ggggttcccc ggagaacggg
      841 gtggcgacga acgtggagac gaactccgcc gcctcgtcga ggctgtgcat ccgcgcgccc
      901 acggccacga cctgcgcgtc gttgtgctgg cggctcagag tcgcagtctc gatgctccag
      961 gcgagcgccg cccggatgcc gggcaccttg ttcgcggcga tctgttcccc gttgcccgag
     1021 ccgccgatga cgacaccgag ggtgccggga tcggcgatgg tccgccgggc cgcctcgatg
     1081 cagaagggcg ggtagtcgtc ctccgggtcg tagctcacgg ggccgacgtc gatcgcctcg
     1141 tggcccagac cggtcaggtg cgcgaccagg tgggccttga gctcgaagcc ggcgtggtcg
     1201 gaaccgaggt agacacgcac gagacgaaat actgccgcat gccatccgta gccggcgtcg
     1261 acggggttcg cgcggatggg tggccgtctc aacggcgtcc ggtgcccttg tcctcggtcc
     1321 tgccgtcggt actctcctct gcccgatccg cccgggccct gtcctcgccc tggccctcgt
     1381 cctcgtcctc gggggcgacg tcctcggtgt gagtggccgg tccggcgggt tcgggggcat
     1441 cagggtcggg cgccgaaccg tccggcaaca gtccgcccgg catggcgagg acggtctgca
     1501 cggtctccag cggcggatcc acccgcggcg gctgcgcttc cccgctcgcc cgcgcgtcga
     1561 cccgttcgct caggtatcgc agcaccaccg ctcccgcggc cgccaccggc acggacagga
     1621 acgctcccgc gatgccgaac agcgtgctgc ccgctgtgac ggcgaggagc acgaccgcgg
     1681 catgcagccc gagactgcgc ccctgcagga ccggctgcag cacgttcccc tcgagctgct
     1741 gcacggccac gatgatcgcc agcacgatga gcgccgtggt gaacccgttg ctcaccagcg
     1801 ccaccaggac tcccagcgcg ccggccacga tcgcgccgac gatcgggatg aacgcgccca
     1861 ggaacgtgag cacggcgagc gggaccgcca acggcacccc gaggatcacc agccccagcc
     1921 cgatcaggac actgtcgacg agtgccacga tcgcctgcac ccggatgaag tcggcgaggg
     1981 tcttccagac ccgccgcagc acctcggcca gatgcccgcc tgcgccctcg cccatcaccc
     2041 ggcgcagcca cggcaggaac cgggggccgt ccttgaggaa caggaacacg agcaccagcg
     2101 tcagcagggc agtgatcact cccgaggcca cactgccgat cccggtgagc acgctcgtgg
     2161 tgatcaccga gatgctctgc tgcagctgcg tcgtcgcgtt ctgcaggacc gcgtcgagct
     2221 gaccctgcgc gaagttgagc ggccgcccgg ccagccactg ctggatcgac tggataccgc
     2281 tggatgcggc gcggctgatc tcggggatgc ttcccgccac cgaggtggtg atcagcgcga
     2341 tgagcccggc gagcaccacc atcccggcga gcagcaccgt ggccgaggcg agcgcgggcg
     2401 ggaagccgtg cctgcgcagc cacgccgtgg gcggccacaa cacggtcgag acgatcaccg
     2461 ccatgatcac gggaaagacg accgaccaca gaagcgcgat caccgtccag accagccagg
     2521 cgcccgctgc caccagcagc agccgaaggc tccaccgcgc cgcccacgtg atgccctcgc
     2581 cgatcgcggt gccgcggccc cggcgcggcc gctcggtcgt ggtcacgaca actcctctgc
     2641 tcgtccgggc ggcgccgcac gtgtgtggca cggacttccc ggtggcccag ccctacccgc
     2701 cgacaaggct ctcagacatc agagatccgc accggacccc gtacgcctac gatcgcgcag
     2761 gtgagcaccg acagcgacgg tccggccgca gggatcgttc tcgcgggcgg gcgctcgtcg
     2821 cggatgggcg agtccaaggc ggcgctggag tggcacggct ccacgttgct gcgacgcacc
     2881 gccggcctgc tggcccgcgc ggtcgacggt ccggtcgtcg tcgtccgggc gcccggccaa
     2941 ccccttcccg cgctgccggc gggcgtcgag gccgtcgacg acccggtgga gggcctcggc
     3001 ccgatgcagg gcatcgccac ggggctcgcc gcggtggcgg accgcgcgca ggtcgcgttc
     3061 gtctgctcca ccgacctgcc gttcctgcac cccgcgtacg tgcggcgggt actggccgcg
     3121 ctggacgccg acgtcgacgt cgtactgccg cacgcccacg ggcaccggca gccgctcgcg
     3181 gcggcgtacc gcacggcgct cgcgcccgtc gtcgccgggc tgctggccga cgggcagctg
     3241 aagcccgcga tgctcttcga caactgtcgg gtgcaccgca ccgacgacgc agacctgctc
     3301 gccgaccccc ggctcggcgc gctggacccg gggctggact cggtgctcaa cgtcaacgaa
     3361 cccgacgact accgcgccgc ccgggcccgc ccggcgcccg agatcaccgt ccagcgctac
     3421 ggcgcgctcg cgtcgaacgg caggcgcggc cccgagtccg tccgggcggc caccctcggc
     3481 gcagccgcag ccgccgtcgg gctggcgctg gaccggcacg tcgtcgccgc cctgaacggc
     3541 gaccagatca cccgcgacgc ggagatgcca ctcgtcgccg gcgacatggt cgccttcctg
     3601 tcggccgacg ccggcggctg atcggcggca gcgccccgaa catcggcata cagtcgtcac
     3661 catgacggca ggcggatatt tcggacgggc cctcgtcgtc gacgtgcgcg gtgacagcgc
     3721 caccgggacg ccgctggagc tggacgaccg ggtcctgcgc gcgtacctcg gtggggtcgg
     3781 gctcggcacc tggctgctgc accgactcgc tcccccgggt gtcgacccgc tggccccgga
     3841 ggcaccactc gcgttcgtct tctccccgct ggtgggcaca ccgctcacga cgagcgccaa
     3901 gttcgcggtg ctggccaagt cgccgctcac cgggatgatc accgatgcgc tggcctcgtc
     3961 ccagttcgcg atctcgggaa agctcaccgg gcacgacgcg atcgtcgtgc gcggccgcgc
     4021 cgacgagctg tcggtggtga tggtcgacgc atccggcgtg cgcctcgagg cagccccgga
     4081 gctggccggg atggccgcgg cggaggcgga gtcggcggtg cgcgagcggt tcggccgggg
     4141 ctggcgaacg gccgcggtcg ggcctgccgc ggagcgcggc gtccggtacg cgacgatcag
     4201 ccacgacggc cggcacgccg gacgcggggg gctgggcgcc gtgctgggcg cgaagaacgt
     4261 caaggccgtg ctggtcaagg ccggtacgag ggtggacgtg gccgaccagc aggccgtcct
     4321 cgccgcagcg aaggacctgc gcgagcgcag cttcggcccg gccaccgcca agtaccggga
     4381 gctcggcacg ctggccaacc tgctcgcctt caacgccgtg tcgacgctgc ccacccgcaa
     4441 cttcaccgcc gccacgttcg ccgaggcacc ccggctcgcc gccgaggagc tgcacgagat
     4501 gcgcggcgtg gcccgcaaca gctgcgcgtc gtgctccatc ggctgcgagc acatctactc
     4561 gcgcaagggc gggggcagcc agcgcatgga gtacgagaac gtgttcgcgc tgggtccgct
     4621 gtgcggggtg tccgaccccg acgacgtgtt cgccgccagc gcccgctgcg acgagctcgg
     4681 catcgacacg atctcggcgg gcggcaccat cgcctgggcc atggaatgcg tggagcgcgg
     4741 cctgatcgac gctccgtggc tgcggttcgg tgacggggcc gcgctgttgc gggcgctcga
     4801 tctgatcggc tcccgcacgg aggggctcgg cgcgctgctg gctatgggct cgcgcgcggc
     4861 cgccgcggtc gtcgggcagg gatcgatcga cttcgcgccg caggtcaagg gcctggagct
     4921 gccagggtac gagcctcggt cgctgcagtc gatggcgctc ggactggcgg tcaacgcccg
     4981 gggcgccgac cacaaccgct ccggcgccta cgaggccgac ctctcgggcg acctggaccg
     5041 gctggccggt ggctccgcac acgtcgccgc agcgatcggc accgaggacc gcgccgccgt
     5101 catggactcg atgattctct gcaagttcct ccgcggggtg ttcacggagc ccttcgacga
     5161 atgggcggca ctgctgcggg ccgtcaccgg ctgggacgtc accgccgacg agctgcgcac
     5221 ggcggcccgc cggatcgtgc tggcgaagcg gctgttcaac ctgcgcgagg gcgccacgac
     5281 cgacgacgac accctgccgc ggcgcatgct cgacaccccg ctcgagctgg gctccggccg
     5341 caccgccacg ctcaccgccg atcgcttgcg gtccatggtc gagggctact acaccggccg
     5401 aggtctggac gtcgaggggc gccccgaccc cgctgacctg gccgacctgc tgctctgagg
     5461 gtcagaacgt gatcaacggg tcgccgagga ggtcggccac gaagttgacg aagaagaacc
     5521 cgaagaacag gaagttgagc agcaggatca cgtagtgcca cggccgcggc cgggccgcct
     5581 tcggcagcct gctgttgagg tagatgagca ggaacgggta gatcaacgcg ccgaggttcg
     5641 acatgttcgc cgagaactgc acgagcgtca ccggcaccgc gaggtgcagg atcaccgaga
     5701 tcgccaccag cagcgcgatc atgaacgggt agtagaagcg gcgagggtcg ccctcgatca
     5761 gcgcacgcat gcgggggctg accgcgggcg cggcgtcggt gaccactcgc accatcgcct
     5821 cgaagatccc gagctgcgtg gagaacagga tgagcacgcc cagcacgagc agcaggtaga
     5881 acatgcccga gccgtactcg gtctgcagcg cgctcgccgc gaacgtgggc acgttggcgg
     5941 ccgtcggctg ctcgcccgac agcgccaccg cctgcgacat cagcaccgtg ggcagcagca
     6001 tgcccagcat cgcgccgacg aagaacacgc cccacatgtc caccatcagc aggcggtacc
     6061 agcggcgcca caggctcgtg ttgcgctcgt cgtcggggaa ggtgacaccc accgacagca
     6121 gctcgcgctg cccgccgcgc atcccggcga tgtagccggt gcggtggccc attccgtagc
     6181 ccttgtcgcg atagtggccc atcacgtacc agttcagccc cgacgccagc gcggtgaacc
     6241 cggctagggc cccgagctgg gtggcggtga tacctgctgg tggggccgcc ggggtgacga
     6301 acccgcggat gccttcccac cacacgctcc acggcacgac gatgaggtcg accacgatca
     6361 gcgtgaccag gatgaagccc accatgaccc agttggacag ttccaacgcc ctgctgatcc
     6421 ggcgggccga tgcagtgatg aggaagacca cgaccagcag cccgatcgcg taccagcgcg
     6481 tctgctgcac ctcggacgca tcggcgaccc ggccgttgac cagcgcgaac aggccctggc
     6541 ccgctgaagc ggcccacccg cccgcgatga aggcgaagat cacgacgaac acggacagcg
     6601 ggatccacag catcttgccc ggtggcacgc gcccccagcc gacgaccggc gcctcgccgg
     6661 tggcgaccac gtaccgcgcg cactcgacgt tgtagaaggt ctgcagcacc gcggacacca
     6721 cgatgaccca gccgaccccg acgaacccgt actggccgac tgcctgcggc ccgagtagcc
     6781 actcgccgct gccgatcgag atgccgagcg cgatgagact cgggccgatc acgaacatga
     6841 ccatctggcg gggaccgatc cggcggaccc ggaagacttc ctccggcgtc ggcaggtcgt
     6901 ccatacggaa atcgggccgg ctcacgccga gcgggtaggt actggcgctg cgctgcacct
     6961 cggtcatgac gcccccgaat gtctcatttg tgagcaatat cactaatggt cgccgaggtg
     7021 ggggcagcgg tcaagcaggg cttgacttga acctcgcttt aacttctagt gttcggcgca
     7081 atcgggaaga accggacgag aactgtcggt cccactcgcc acactggcga ggcggagttg
     7141 ttccgcaccg atgagttccg ccggctcccc atgtcctatg ggtgcacggc gggtgcggcg
     7201 aaccggaacg aggagcagct catgagcatg gagatggtgg cgtggcactc gatgtaccac
     7261 gcggcgaacg cccaggagga gaagcgccct ttctcgatgg ccacgctgcg ccggatcgtc
     7321 gcgttcgtgg ggccgcaccg ccgccgcatg gccacgttcc tgctggtcag cgtgatcggc
     7381 gcggttctcg cggtcgccac accggtgctc gcgggccggg tcgtggacgc catcgtcaag
     7441 ggcgggccga tgggcgtcgt gatcggcctg gccgggctca tcgcggtcat cgcggtggcc
     7501 gtggccgtca gcgggatcgt gcagcgctgg ctctcggcgt cgctcggtga agggttgatc
     7561 ctcgacctgc gcgccgcagt gttccaccac gtgcagcgcc agccgatcgc gttcttcacc
     7621 cgtacccgca ccggcgcgct ggtgagccgg ctcaacaacg acgtcatcgg cgcccagcgc
     7681 gcgttcagcg acacactgtc cggcgtcatc ggcaacctcg tcacgctcac gctcacgctc
     7741 gtggtgatgt tgacgctgtc gtggcaggtc acgctgctcg cgctcgtgct gctgccggtg
     7801 ttcgtcctcc cggcccgccg gatgggccac cggctcgcct acctggagcg cgaggcggcc
     7861 gaccacaacg cagccatggg cacccagatg accgagcggt tctcggcccc gggcgccacg
     7921 ttgatcaagc tgttcgggcg gcctgccgag gagacgcgcg agttcgtcgc ccgggccgcc
     7981 cgcgtccgcg acatcggcgt gcgcacggcg atggtgcagt gggtcttcgt cacggcgctc
     8041 atgctcgtct ccgcgctggc gctcgcgctg gtctacgggc tcggcgggta tatcgcgctc
     8101 accggcggga tggccgcggg tgacgtcgtc gcgctcgccc tgctgctcac cggcctctac
     8161 gccccgctca cggcgctggc cagtgcacgc gtcgaggtga tgagtgcact ggtgagcttc
     8221 gagcgcgtgt tcgaggtgct cgacctgccg ccgctcatcg cggagcccgc cgacccgctg
     8281 ccgctgcccg aggggccgct ctcggtggag ttcgacgggg tgcgcttcgg ctaccccgcc
     8341 gccgacaagg tgtcgctggc gtcgctggag gaggtcgcac tgctcgacgg caggggcggc
     8401 gaggaggtgc tgcacgacgt gtccttcacg gccgagtccg ggcagatgat cgcgctcgtg
     8461 ggctcgtccg gcgccggcaa gtcgacgatc gctcagctgc tcccccggct ctacgacgtc
     8521 gacgcgggtg cggtgcggct gggcggggtc gacgtgcggc ggctctcgtt cgacacgatc
     8581 cgcggggcgc tgggcctggt cacccaggac ggccaccttt tccacgagtc gatccgctcc
     8641 aacctcctcc tcgcccgccc ggaggcccac gacgacgagc tctgggaggt gctgcgccgg
     8701 gcccgcctcg ccgacctggt ggcctcgctg cccgacgggc tcgccaccgt cgtcggcgag
     8761 cgcggatatc ggctgtccgg cggcgagcgc cagcggctca ccatcgcgcg gctcctgctc
     8821 gcccgtcccc gggtggtggt gctcgacgag gccaccgccc acctcgactc cacctccgag
     8881 gccgccgtgc aggaggcgct gggcgaggcg ctcctcggcc gcaccgccat cgtcatcgcc
     8941 caccggctct ccaccatccg ggccgccgac cagatcctcg tgctggaggc gggccagatc
     9001 gtcgaacgcg gcacccacgc cgaactgatc gcacaggacg gccgctacgc agaactacac
     9061 cgcacccagt tcgcccagcc ggaggtggcc gccccggtag ccctagcggg gatctgaggg
     9121 gtgatcctcg ctgaggggtg tgctcttcgc agggccggcg ggtcctgcga agagcacgcc
     9181 aggtcaccgg ggaccgctca tacctcgggg aaagccacga accgcaccag gacgtggcga
     9241 gc
//
LOCUS       DASRSC010000253         1878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4848130,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000253 DASRSC010000000
VERSION     DASRSC010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4848130"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1247
                     /locus_tag="VFE39_12415"
     CDS             <1..1247
                     /locus_tag="VFE39_12415"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HET6259446.1"
                     /translation="TPDVVVTGLGATTPLGGDVASTWEALLAGRSGVRSIDDADHEWV
                     ERHQLPVKIAAPLAVAPTEVLPRVQARRLDRCEQVALVAAKEAWAHAGLGDRDDMQVE
                     PERLAVVVGTGIGGAVTLLDQDDLLENSGLRKVSPLTVPMLMPNGPAAWIGIEYGAQG
                     GVHAPVSACASGAEALAWALRLLRGGEVDVVIAGGAEACITGITVAGFVQARTLSQRN
                     DEPERASRPFDVDRDGFVLGEGAGIMVLERADFAAARGATVHGRLAGAGLTSDAYHIT
                     GPEPEGVGQSRAIAKAVRDAGLDPADVGHVNCHATSTVAGDVGETKAVRKALGDHPIL
                     TAPKSALGHMVGAAGAVESIITLLSIKEGVIPATLNLENLDPGVELDVVAGEPRKVDL
                     DAAVCDSFGFGGHNAAVVFTSP"
     gene            complement(1333..1821)
                     /locus_tag="VFE39_12420"
     CDS             complement(1333..1821)
                     /locus_tag="VFE39_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3145 domain-containing protein"
                     /protein_id="HET6259447.1"
                     /translation="MNARGVVFVHSSPTAVCPHVEWAVSGVLGTKVSLQWTPQPAVQG
                     QMRAECSWTGPTGSGGALAGALRAWPMLRFEVTEEPSQGVDGERFCHVPTLGLWSGRT
                     SANGDIVVGEDRLRALLVEVSATQLPARMHELLGTAWDDALEPFRLAGDGAPVTWLHQ
                     VG"
BASE COUNT          274 a          684 c          669 g          251 t
ORIGIN      
        1 ccactcctga cgtcgtcgtc accgggctcg gcgcgacgac cccgctcggc ggggacgtcg
       61 cgtcgacgtg ggaggcgctg ctcgccgggc gttccggcgt ccggagcatc gacgacgccg
      121 atcacgagtg ggtcgagcgc caccagctgc ccgtgaagat cgctgcaccg ctggccgtcg
      181 cgcccaccga ggtactcccc cgcgtccagg cccgcaggct ggaccggtgc gagcaggtgg
      241 cgctcgtcgc ggccaaggag gcatgggccc acgccggcct gggcgaccgg gacgacatgc
      301 aggtggagcc cgagcgactc gcggtggtgg tcggcaccgg catcggcggc gcggtcaccc
      361 tgctcgacca ggacgacctg ctggagaaca gcggcctgcg caaggtctcg ccactcaccg
      421 tgccgatgct gatgccgaac gggccggcag cctggatcgg catcgagtac ggcgcccagg
      481 gcggcgtgca cgcgccggtc tcggcgtgcg cgtccggcgc ggaggccctg gcgtgggcac
      541 tgcggctgct gcgcggcgga gaggtcgatg tcgtcatcgc cggtggcgcg gaggcctgca
      601 tcaccggcat caccgtcgcc gggttcgtgc aggcccgcac gctgtcgcag cgcaacgacg
      661 agccggagcg ggcctcccgg ccgttcgacg tcgaccgcga cgggttcgtg ctcggtgagg
      721 gtgccgggat catggtgctc gagcgcgcgg acttcgcggc ggcccgcgga gccaccgtgc
      781 acggcaggct cgccggcgcc ggtctcacgt cggacgcgta ccacatcacc ggcccggagc
      841 cggagggcgt cgggcagtcg cgggccatcg cgaaggcggt ccgcgacgcc gggctggatc
      901 cggccgacgt cgggcacgtc aactgccacg ccacctccac ggtggccggt gacgtgggtg
      961 agacgaaggc ggtccgcaag gcgctcggcg atcacccgat cctcaccgcg ccgaagtcgg
     1021 cactgggtca catggtcggc gcggcaggcg ccgtcgagag catcatcacg ttgctctcga
     1081 tcaaggaggg cgtgatcccg gcgacgctca acctcgagaa cctcgacccc ggcgtcgagc
     1141 tcgacgtagt ggcgggcgag ccgcgcaagg tcgacctgga cgccgcggtc tgcgactcgt
     1201 tcgggttcgg cggccacaat gcggccgtgg tcttcaccag cccgtgaacg gaaagcgttg
     1261 ctgccgggcc gatgagcgga cgccagggcc ggagcaacgc tttccagtca cgctctaccc
     1321 cacctgatcg cgctacccca cctgatgcag ccaggtgacg ggcgcaccgt cgcccgccag
     1381 ccggaacggc tccagcgcgt cgtcccacgc cgtaccgagc agttcgtgca ttcgggcggg
     1441 gagctgggtc gccgacacct cgacaagtag ggcccgcagc cggtcctccc ccaccacgat
     1501 gtcgccgttg gcgctggtcc gcccgctcca gaggccgagt gtgggtacgt ggcagaaccg
     1561 ctcgccatcg accccctggc tgggttcctc ggtcacctcg aaccgaagca tcggccaggc
     1621 gcgcaacgct cccgcgagcg cgccaccaga accggtcggc cccgtccagc tgcactcggc
     1681 ccgcatctga ccctgcaccg caggttgtgg agtccactgc agggagactt tggtcccgag
     1741 cacgccagag accgcccact cgacgtgcgg acacaccgca gtcggcgacg agtggacgaa
     1801 gaccacgccg cgtgcgttca ctgcagacct ccaacgtcga cgaggatcgt cttcccctac
     1861 gtcctcgcgt cgcggcgg
//
LOCUS       DASRSC010000254         4672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2390292,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000254 DASRSC010000000
VERSION     DASRSC010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2390292"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..964)
                     /locus_tag="VFE39_12425"
     CDS             complement(194..964)
                     /locus_tag="VFE39_12425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6259448.1"
                     /translation="MDDVLQRIDRFCDEVPRHWATPLDDGPLRLFVRNRPGWPFYARP
                     VPGGPPVTAEDVERVRARQRALRVPEAFEWVAGEAPSMADAARAAGLAVKMCPLLVLD
                     GAPVGPPLPAGYSARLVGPADGDLQAAAHSVLTVAAAAFGAPSPGPPVGAALAELRAD
                     LAAGAIARALVTGPDGPVTAGSAQRVGDVVELVGIGTVAAARGRGLGGAVTALLAGAA
                     RDAGAEVVFLAAGDAAATRVYERVGFRRVGECGIAESG"
     gene            complement(985..2625)
                     /gene="cimA"
                     /locus_tag="VFE39_12430"
     CDS             complement(985..2625)
                     /gene="cimA"
                     /locus_tag="VFE39_12430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HET6259449.1"
                     /translation="MSRTEPAETPLGDAFHVYDTTLRDGAQREGISYSVADKLAVARH
                     LDALGVGFIEGGWPGAMPKDTEFFARAASGELRLRHAALVAFGATRRAGLTAAADPQV
                     RALLDSAAPVVTLVAKSDRRHIERALRTDVRENCAMVADTVAFLRGEGRRVFLDAEHF
                     FDGYRFDPETALRVLDAAVGAGADVAVLCDTNGGMLPLGIAEVVTEVAERTGFRLGIH
                     CQDDTGSAVANSVTAVQAGATHVQCTANGYGERTGNADLFAVVGNLVTKLNMPVLPDG
                     AFGELTRTSHALAEIANIAPDTHQAYVGTSAFAHKAGLHASAIKVDPELYNHMDPVAV
                     GNGQRVLVTEMAGRASVELKGVELGLDLAGRPDVVSSVVATVKEREAQGWSFEAADAS
                     LELLMRSALGGADRVPFALESYRVIVEHGADGDVVAEATVRVVLADGQRLIATAEGNG
                     PVNALDAALRGALTPSCPWLSDVELSDYKVRILTTGRSDSYGTDAVTRVLVESTNRSG
                     SWTTVGVHSNVVEASWLALVDALAYAALRAPVPAGAGS"
     gene            2911..3219
                     /locus_tag="VFE39_12435"
     CDS             2911..3219
                     /locus_tag="VFE39_12435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HET6259450.1"
                     /translation="MFQLTVLYNQPEDPAAFDKHYDEVHAPLAATIPGLQRYTVSRPG
                     PDADGNQPPYHLVAVLEFESQETFGAGMSGPEGQAAVADLPNFAGAGTTMLTGEATTV
                     "
     gene            complement(3227..4270)
                     /locus_tag="VFE39_12440"
     CDS             complement(3227..4270)
                     /locus_tag="VFE39_12440"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HET6259451.1"
                     /translation="MRLAVIPGDGIGPEVIDEALKVLNEVEPGAATTNYDLGAARWHS
                     TGELLPESVLTELQEHDAILLGAVGDPSVPSGILERGLLLRLRFELDHHVNLRPARLY
                     PGVRGPLSGNAEIDMLVVREGTEGPYVGTGGLLRKDTPHEIATEVSVNTAFGVERVVR
                     DAFARATRRPRRHLTLVHKTTVLSYSGRLWSRLVEEVSLEHPEVSVAYQHVDATTIHM
                     VTDPGRFDVIVTDNLFGDILTDLAAAVTGGIGLAASGNLDVSRRNPSMFEPVHGSAPD
                     IAGQGIADPTAAVLSVALLLDHLGNRDAARRIEAAVAFDLATRDHSATGHTQSIGDRL
                     AALVSAHATTNAS"
BASE COUNT          665 a         1713 c         1645 g          649 t
ORIGIN      
        1 caggtgtaac agcggacatc tgctctgccc gctcaccgtg atcaaccgga tgaaattcgt
       61 gatccgctgc cggtccagca ggcccgcggg cgttgcgtcg accgtgtcgc ccgcgtcgcg
      121 catgtcggcg gcgacccggt ccaggtcgac catcgcgtcc aggcccccac cgtgatcgat
      181 gtctgaccag ctctcagccc gactcggcga tgccgcactc gcccacccgc cggaatccga
      241 cgcgctcgta gacgcgggtg gcggcggcgt cacccgcggc gaggaacacc acctccgcgc
      301 ccgcgtcccg ggcggccccg gccagcagtg ccgtcaccgc tccaccgagt ccccggccgc
      361 gcgcggctgc cacggtgccg atcccgacca gctcgaccac gtccccgacg cgttgcgccg
      421 atcccgcggt gaccggcccg tccgggcccg tgaccagcgc tcgcgcgatc gcccctgcgg
      481 cgagatcggc acgcagctcc gcgagggcgg cgccgaccgg tgggccggga gacggggcgc
      541 cgaaggctgc agcagcgacc gtcagtacag agtgcgccgc cgcctgcaga tcgccgtcgg
      601 ccggcccgac caggcgcgcg gagtagccgg ccgggagtgg ggggcccaca ggggcgccgt
      661 cgagcacgag cagcgggcac atcttcaccg ccaggcccgc cgcgcgggcc gcgtccgcca
      721 tcgacggggc ctcgccggcg acccactcga acgcctccgg cacgcgcagc gctcgctggc
      781 gtgcccgtac ccgctcgacg tcctctgcag tgaccggcgg accgccgggg accgggcggg
      841 cgtagaacgg ccagcccggg cggttgcgga cgaagagccg cagcgggccg tcgtcgagag
      901 gggtggccca gtgccgtgga acctcatcgc agaaccggtc gatccgctgc agcacatcgt
      961 ccacgggctg atcctgccct ggcgtcagct cccggcgccc gcggggacgg gagcgcgcag
     1021 cgccgcgtag gccagcgcgt cgacgagagc gagccaggac gcctcgacga cgttgctgtg
     1081 cacgcccacg gtggtccacg aacccgatcg gtttgtggac tccaccagca cacgcgtgac
     1141 cgcatcggtg ccgtagctgt ccgaacgacc ggtggtgagg atgcggacct tgtagtcgga
     1201 cagctccacg tccgagagcc acgggcacga cggtgtgagc gcgccccgta gtgccgcatc
     1261 gagcgcgttg accggcccgt tgccctccgc ggtcgcgatc agccgctgcc cgtcggccag
     1321 gacgacccgc accgtcgcct cggcgacgac gtcgccgtcg gcaccgtgct cgacgatcac
     1381 gcggtaggac tcgagggcga agggcacccg gtcggccccg cccagggctg agcgcatgag
     1441 cagctccagc gaggcgtcgg ccgcctcgaa cgaccaaccc tgcgcctcac gctccttgac
     1501 ggtggccacc accgacgaca ccacgtcggg acggccggcc aggtccagac cgagctcgac
     1561 acccttgagc tcgacgctgg cccggccggc catctccgtg accagtaccc gctgcccatt
     1621 gccgaccgcc acagggtcca tgtggttgta cagctccggg tccaccttga tcgcactcgc
     1681 gtgcaggccc gccttgtggg cgaacgctga cgtcccgaca taggcctggt gggtgtcggg
     1741 agcgatgttg gcgatctcgg ccaacgcgtg cgaggtgcgg gtcagctcgc cgaacgcgcc
     1801 gtccggtagg acgggcatgt tcagcttggt caccaggttc ccgacgacgg cgaacaggtc
     1861 ggcgttgccg gtccgctcgc cgtagccgtt cgcggtgcac tgcacgtgcg tcgcgccggc
     1921 ctgcaccgcg gtgaccgagt tggccaccgc gctgcccgtg tcgtcctggc agtggatgcc
     1981 gaggcggaag ccggtgcgct cggccacctc ggtcacgacc tcggcgatgc ccagcgggag
     2041 catcccgccg ttcgtatcgc acagcaccgc gacgtcggcg ccggcgccga ccgcggcgtc
     2101 cagcacccgc agcgccgtct ccgggtcgaa gcggtagccg tcgaagaagt gctcggcgtc
     2161 gaggaacacg cgcctgccct caccgcgcag gaacgccacc gtgtcggcca ccatcgcgca
     2221 gttctcccgg acgtcggtgc gcagcgcccg ctcgatgtgg cggcggtcgg acttcgcaac
     2281 cagcgtgacg accggcgccg cgctgtcgag cagggcgcgg acctgcgggt cggccgccgc
     2341 cgtgagaccg gcgcgccgcg tcgccccgaa cgcgacgagc gcggcgtgcc gcagccggag
     2401 ctcccccgac gcggcgcggg cgaagaactc cgtgtccttc ggcatcgccc ccggccagcc
     2461 gccctcgatg aagccgaccc ccagcgcgtc gaggtgccgg gcgacggcca gcttgtcggc
     2521 caccgagtag ctgatcccct cgcgctgggc gccgtcgcgc agcgtcgtgt cgtagacgtg
     2581 gaacgcgtcg ccgagcgggg tttcggctgg ttcggtgcga gacatccggg gtgctcctgg
     2641 gggctcggga cgtgatcggg caacaaaaaa gaccccccgc gaatgcgaga ggtctgcgcg
     2701 tcggctgtgg tgctggttag ccgacgcgcc tgtcgataat gatcacgctg aagtgctgca
     2761 ccgtgtcagc atggcacgga tcgtcccggg atgtcacacc gccgtcccgg atagtggaca
     2821 cgctatgtcc acagagggcg cgctccgcta ctgattaggc gcggagcctg cggacaccgt
     2881 ggtccgtggg cccgtctctg gaggtgctgc gtgttccagc tcaccgtgct ctacaaccaa
     2941 cccgaggacc ctgccgcgtt cgacaagcac tacgacgagg tgcacgcccc gctcgccgcc
     3001 acgatccccg gcctgcagcg ctacacggtg agccggcccg gcccggacgc cgacggcaac
     3061 cagcccccgt atcacctcgt cgccgtactc gagttcgagt cgcaggagac cttcggtgcc
     3121 ggcatgagcg gcccggaggg tcaggccgcc gtcgccgatc tcccgaactt cgccggcgca
     3181 ggtacgacga tgctgacggg cgaggccaca accgtgtgac ggcgcgtcag gaagcgttcg
     3241 tcgtggcgtg ggcgctcacc agggcagcga gccggtcgcc gatactctgc gtgtggcccg
     3301 tggcgctgtg atccctcgtg gccaggtcga acgccaccgc ggcctcgatg cggcgagcgg
     3361 cgtcccggtt gccgaggtgg tcgagcagca gcgccaccga gagcaccgcg gccgtggggt
     3421 cggcgatgcc ctgcccggcg atgtcgggcg cgctgccgtg caccggctcg aacatgctcg
     3481 ggttgcggcg gctcacgtcg aggttgccgc tggccgcgag cccgattccg cccgtgacgg
     3541 ctgccgctag gtcggtgagg atgtcaccga acaggttgtc ggtgacgatc acgtcgaacc
     3601 ggccgggatc ggtgaccatg tggatcgtgg tggcgtccac gtgctggtag gccacgctga
     3661 cctcggggtg ctcgagtgag acctcctcca ccagccgcga ccagagccgc ccggagtagc
     3721 tcagcacggt ggtcttgtgc accagcgtga ggtgccggcg cggccggcgg gtggcgcggg
     3781 cgaacgcgtc gcggaccacc cgctccacgc cgaaggccgt gttgacgctg acctccgtgg
     3841 cgatctcgtg cggggtgtcc ttgcgcagca ggccgccggt gccgacgtac gggccctcgg
     3901 tgccctcgcg gacgacgagc atgtcgatct cggcgttgcc ggacagcggg ccgcggacgc
     3961 ccgggtagag ccgtgcggga cgcaggttca cgtggtggtc gagctcgaag cgcaggcgca
     4021 gcagcaagcc ccgctcgagg atgccgctgg gcacggaggg gtctcccacg gcgccgagca
     4081 ggatcgcgtc gtgctcctgc agctccgtga ggaccgactc cggcagcagc tcgcccgtcg
     4141 agtgccaccg tgcggcgccg aggtcgtagt tcgtggtcgc cgcaccgggc tcgacctcgt
     4201 tgaggacctt cagcgcctcg tcgatgacct ccggcccgat gccgtcgccg ggaatgactg
     4261 caaggcgcat tgccgatgtc ctctctccac gtgaccccga agcagccggg ggcacgttac
     4321 cgactgcaac ggcgccgcta cgccgtccgc cacccgatcg gggtcgcccc aacggatgga
     4381 actgctgcgg aagcgagcca gacgggccgt gattgcgccc cgacacacca ccaaccgggc
     4441 cattctgccg tgcgcatcag aatgcctgcc cgccgccccc gcactcgcga ctgggccatc
     4501 gggcatttcg ggtggtcggg acgagctctc gcggccgaga gccctcaccc gttcgccgcg
     4561 tcgcgccact acccggtgac ccctgtcgca taccgggtgc cgatactgct gcaggagtga
     4621 ccggtgccgc taccgggcct caaccgatca ggtgagtgca ccgtccattc gg
//
LOCUS       DASRSC010000255        13006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3210793,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000255 DASRSC010000000
VERSION     DASRSC010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 13006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13006
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3210793"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..667)
                     /locus_tag="VFE39_12445"
     CDS             complement(2..667)
                     /locus_tag="VFE39_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6259452.1"
                     /translation="MGGIVGTAERNGARERFDKRQAILRAAITVFAREGYAQAGVDVI
                     AAEAGVAQPTVYNHFEDKETLFRQAIAADSDRALAENLAAVERLQDQDGDVRPMLEDV
                     AHRLLVCYCDDRSWALRRLLYIELTRFPDLIDVVRESAADRVTDALADKLARLSLAGR
                     LGKLDPDEAAEQLIALLTGPMEARARLGTRRVPDDDLRAVARAAVRTFLHAFAAKPDE
                     YGR"
     gene            complement(819..1208)
                     /locus_tag="VFE39_12450"
     CDS             complement(819..1208)
                     /locus_tag="VFE39_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6259453.1"
                     /translation="MLKIHIASVFVDDQAKAETFYVDVLGFVKKNDIPLGEARWLTVV
                     SATESNGTELLLEPDGHPAVGPFKKALVADGIPFTSFAVEDANAEFERLRSAGVHFVQ
                     EPVNMGPVTTAVFDDTCGNLIQIASEN"
     gene            complement(1219..1491)
                     /locus_tag="VFE39_12455"
     CDS             complement(1219..1491)
                     /locus_tag="VFE39_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6259454.1"
                     /translation="MSALFKALVDPTRRAILDELTDKDGQTLFEICSRLTMKHGLGSS
                     RQAISQHLAVLEEAGLVTTRREGRYKFHHLDTGPLRSIIERWPIDP"
     gene            1570..2919
                     /locus_tag="VFE39_12460"
     CDS             1570..2919
                     /locus_tag="VFE39_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HET6259455.1"
                     /translation="MLDWTKHVIWWHVYPLGFTGAEGSAADAPHPVPRLRALEPWLGY
                     LLDLGCNGLALGPVFASGTHGYDTTDHFRIDPRLGTSDDLRWLLDTCRERGVHVLLDG
                     VFNHVGRDFPQFRDVLEHRGASRWANWFDLDLEAGGPDGFGYRDFEGHHELVALNHAE
                     PEVIDHITTVMNHWLDVGASGWRLDAAYAIPQDALRAISDAVHAEHPDAWLVGEVIHG
                     DYPSFVRTGGLDSVTQYELWKAIWSSLNDRNLFELAWALQRHDALLDDLVPLTFVGNH
                     DVTRLASRLTDPELLGHALAVLFGVAGVPAVYYGDEQAFRGVKEDRAGGDDAVRPAFP
                     ETTDGLAPHGWPVYRTHQELIGMRRRNAWLTGARTAVDHVANEQLAFTSRSGDGEVSV
                     LLNLADREHRFDPRGDAQVLASSGSGGDSWLLAPRSWAVLAGNEKSDGTVSTATDQF"
     gene            2970..3662
                     /locus_tag="VFE39_12465"
     CDS             2970..3662
                     /locus_tag="VFE39_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6259456.1"
                     /translation="MDASPTFVLIPGAGGSAFYWHRLVEELHRRGRAVIAVDLPAGDD
                     SAGLAEYTDAVVAAIGDRSRIVLVAQSLAGFTGPLVCERVPVELLVLLNAMVPTPGEA
                     PGDWWENTGQAQAAAQLAEREGRSSGDGFNPAVEFFHDVPPEVTAAVFAQPEPVQCGT
                     PFEKPWPLERWPDVPTRFLQGRDDRFFPLEFQRRVVRERLGIPVDEMPGGHLVALSRP
                     RELADRLEAYLS"
     gene            3799..4074
                     /locus_tag="VFE39_12470"
     CDS             3799..4074
                     /locus_tag="VFE39_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6259457.1"
                     /translation="MAAAGEAWAKEMDGRGVRLQGNRLRPIADATTVRVRETEVLVSD
                     GPFAETKEQILGYDLLDCQDLDEAIEVASKHPVARYGSIEVRPFWTG"
     gene            4071..5366
                     /locus_tag="VFE39_12475"
     CDS             4071..5366
                     /locus_tag="VFE39_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6259458.1"
                     /translation="MTGVEAAAVEAAVVAAFRAEWGRVVATLIRMTGDWDLAEECAQE
                     ALAQAVARWPRDGVPRSPGAWLTTTARNHALNRLRRTALEAAKLREAAMEPIQGDQDD
                     GSAVQDDRLRLIFTCCHPALPLEGRVALTLRSLAGLTTAEIARAFLVSESTMAQRLVR
                     TKRKIREAGIPFRVPPDHLLPDRTAAVLGVIYLLFNEGYAAAAGSELLRHDLCAEAIR
                     LARTLARLMPDEPEVLGLAALLLLQDSRRGARLDDTGEPVTLEDQDRSRWDAAAIAEG
                     LELLDAALRRGGPGRRPAGPYQVQAAIAACHARAGRAVDTDWPQIAALYGRLVEMTSS
                     PVVRLNRAVAVAMADGPAAGLELVDALGGEPAGYHLLPATRADLLRRLGRYGDAADAY
                     RDALRLTGTDAERRFLTRRLAEVSPPPPLASTGTPAGRL"
     gene            complement(5338..5850)
                     /locus_tag="VFE39_12480"
     CDS             complement(5338..5850)
                     /locus_tag="VFE39_12480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259459.1"
                     /translation="MPTTTTSAPALFSPEWAEAVRDAVDRGPSDELRAEKLPTYWEWI
                     DEVRAGYDDSWALVDPDLPAEFGGGPAHLLLQWERGRCTRAAIVGPEEAAGATYVLRA
                     THEAWRELLDGADAGRMLIYRRVRLERGDVLQFFRGVYFVVEALAALGRVPARLDEVG
                     RLTDDRQAYQ"
     gene            complement(5853..6815)
                     /locus_tag="VFE39_12485"
     CDS             complement(5853..6815)
                     /locus_tag="VFE39_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase"
                     /protein_id="HET6259460.1"
                     /translation="MVTRQETVQERVANSTLAQIRDVDIDDVLDYLHADLKKLPDYLD
                     LYRRYLRQRWDVYELDFDQDAIDWRTRMSAEERAAFVAISSGFHHGERQVEVELPVFM
                     LGGSEESKIYMSSQIEDEARHTIFFDRFYREVVALPGTGIQDVLDASYPHVSETFVGP
                     FGLLAHQADELRRDPEDPAARVRYGTTYFLWIEGVLALSVMKITLSYCRTRKLLPAYY
                     SGFTATCRDEARHVQGGMRILRDAVQSDPAMLHEIHETLRTILRMNGAVSRRLALESL
                     GWTPEEVRRLMIRQLTMKLSDVGIDLPPDLREQIDRLQPELAGG"
     gene            complement(6882..8618)
                     /locus_tag="VFE39_12490"
     CDS             complement(6882..8618)
                     /locus_tag="VFE39_12490"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HET6259461.1"
                     /translation="MSGVPVSGAGADPSDGTSEPGRLSPAGRRAAVAAARTGRFDVVV
                     VGGGVTGAGAALDAASRGLSVVLVEAGDLASGTSSRSGKTVHGGLRYLEQLNFSLVAE
                     ALRERDLMVRTTAPHLVTPEPFLVPFTTPWERPYIGAGVLLYDLMAAAGRTGGRGGVP
                     HHRHLTRRGALREAPGLAADVVTGAVQYYDGRMDDARHTLAVARTAAALGAHVLSHAR
                     VVGVLREGPRVAGVVVEDALSGDRFDVTASVVINAAGVWAADVQAMAGSASFAVRPAK
                     GVHLLVEQGAFDSRTGLLARAEDSVIILRRWFGHWLLGTTDTTYTGDRRAPTVEQADV
                     DYLLRNVNRYLGRPLSRSDVLGAFAGLRPLLAPVTRDAATTSALSRDHTVVAEPAGMV
                     TVVGGKYTTYRRMARDAVDAAGCELGRALPPSPTAEMPLLGAAGWRAVGNRVPRLAAE
                     HGVPADHVHRMLHRYGAELPAVLAPVRDDPALGRPLRGAEGYLPVEFRYAVTHEGAAT
                     LSDVLTRRTHLVIERTDGGTAAAPAVAALLAPLLGWDETRQAREVAEHRAEVERDRTA
                     LASAGPAPIDLR"
     gene            complement(8615..10099)
                     /locus_tag="VFE39_12495"
     CDS             complement(8615..10099)
                     /locus_tag="VFE39_12495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET6259462.1"
                     /translation="MNPPTWLGVDAGTSVVKAALFDEDGGALAVAGRPLDLVHGADGA
                     VEQDLEHVLAHLGEVVQEVVATAGRSPSVVALTGQGDGLWLTDAAGAPVRPALSWLDG
                     RASELLGGWTADGVTERVFRTNGTTLFPGAPGPLLGWLDAHEPASLDAAATAGTCKDA
                     LFMRLTGVRATDPSDSSLPFGDGTGTGYSDAVLDAMGLAHRSNLLAPISTPVPQAELS
                     AAGAALLGLPVGVPVSSGPFDFPACAFGGGVRGIGDGLLIVGTTLGCLVHVDRLATDG
                     EPAGFSVATGEPGRWLRAMPAMVGTASMDWMLTTLGLGVEAIDAALGTSPPGAGGVEV
                     LPYFATSGERAPFVDPEACGQVTGVRLTTTRDDLVRAMCEGLAYAARHCFEAAGGVAR
                     LVACGGGGRSRPWLQVFADVLGVPLELARTPEVGARGAVLAAAAARGEPLDVGTWTAA
                     QGIVEPDGTVQAHYDDGYARYLDHVRAAQPFWATRRASLATVSR"
     gene            complement(10096..10743)
                     /locus_tag="VFE39_12500"
     CDS             complement(10096..10743)
                     /locus_tag="VFE39_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET6259463.1"
                     /translation="MLLHDERVQLVQYCRRMQADKLTVGTSGNISVRSGDHIAITPSG
                     AAYEDLTPESICVIDLDGTPVEDGLEPSSEVQMHTSVYRHTSAQAVVHTHPLYASTLS
                     AVVDELPPVHYMVALLGGPVRVAPYATYGSSELAENSVRAMEGRFGVILQNHGATTYG
                     DSLAKAYTRSVYLEWVCQMYYQARLLGEPNLLQLDEIARVAEKLGSYGQSVPGRS"
     gene            complement(10788..11618)
                     /locus_tag="VFE39_12505"
     CDS             complement(10788..11618)
                     /locus_tag="VFE39_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET6259464.1"
                     /translation="MVEVAGHSDEPSENTPPARALGPEGRQARITDLVLRRGTVSAQE
                     LAEAFGVSVMTIHRDLDELQRQGVLRKARGVATARPSGTFESNVEFRAKASVEAKRMI
                     AQHACRHVEPGMSVLIDDSTTAAQMLPHLAALAPLTVATNYLAALVELARLRDVQVMA
                     LGGHYDVQHDSFLGSGCIDAVRSMRFDVAFVSTSAVSEGYAFHQEDKIVAVKRAMVEV
                     AARTHLLIDHSKLTRSALHRLLPLHRFASVIVDADVPARDLAALRENDVRVEVAGRAE
                     "
     gene            11912..>13006
                     /locus_tag="VFE39_12510"
     CDS             11912..>13006
                     /locus_tag="VFE39_12510"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET6259465.1"
                     /translation="MPGPEPADDPAADALWLGVDIGTQGVRAVLVDGAGTVLGTGSAP
                     LHRDVRDGDRHEQDPAEWWSATCTATRAALAGPVGRRVGAVSIDSTSGTLVVQSPSGL
                     PAGPGLMYDDRRAALEAARVQEVGDALWNALGYRMQASWALPKVVQLAGRGAVRAGHR
                     FAHQADHIAARLAGHPVPTDWSHALKTGYDLLGDTWPTAVLDALGVDPALLPPVVTPG
                     QRVAVVSAAAAEASGIPAGTPIAAGMTDGCAAQVATAALAPGEWCSALGTTLVLKGST
                     RELLRDPTGAVYCHRNPDGGWLPGGASSTGAGVLSRDLAGEDLDAVTERARHRGVPSG
                     ATYPLVGTGERFPFVAHDAAGFDVGGPVDGD"
BASE COUNT         1808 a         4610 c         4734 g         1854 t
ORIGIN      
        1 gctacctgcc gtactcgtcg ggcttcgcgg cgaaagcgtg caggaaggtg cgcaccgcgg
       61 cccgtgcaac cgcccgcagg tcgtcgtccg ggacgcgccg ggtgcccagc cgggcacgtg
      121 cctccatcgg gccggtgagc agcgcaatga gctgttcggc ggcttcatcc ggatcgagct
      181 tgcccagcct cccggccaag gagagccgcg cgagtttgtc ggccagtgcg tcggtgacgc
      241 gatccgccgc gctttcccgg acgacgtcga tcaggtcggg gaaccgggtg agctcgatgt
      301 acaacaggcg ccgcaacgcc cacgaacgat cgtcgcagta gcacacgagt aggcggtgcg
      361 ccacgtcctc cagcatcggg cgcacgtcgc cgtcctggtc ctgcagtcgc tcgaccgcgg
      421 ccaggttctc ggccagcgcc cggtcggagt cggctgctat ggcctggcgg aacagggtct
      481 ccttgtcctc gaagtggttg tagacggtgg gttgggccac accggcctcg gccgcgatga
      541 cgtccacccc ggcctgggcg tatccctcgc gggcgaacac ggtgatggcg gccctcagga
      601 tcgcctgccg cttgtcgaag cgctcacggg cgccgttgcg ctcggccgtg ccgacgattc
      661 cgcccacgcc ggcaccgtac agcccaactt gaacccgtga gttcactcag ctgggcggtc
      721 atcgccgccc gcggcacaag agcccgtgcg cggatacgag tgctggaaca ctcgtatccg
      781 cgcacggccg gggtcagggg aggctgcagg gtcggcggtc agttctcgct ggcgatctgg
      841 atgaggttgc cgcaggtgtc gtcgaagacg gcggtggtca ccgggcccat gttcacgggc
      901 tcctgcacga agtggacgcc cgccgaccgg aggcgttcga actcggcgtt cgcgtcctcc
      961 accgcgaatg aggtgaacgg gatgccgtcg gccactagtg ccttcttgaa cggcccgacg
     1021 gccgggtggc cgtccggctc cagcagcagc tccgtgccgt tcgactccgt ggccgacacc
     1081 accgtcagcc accgggcctc acccagcggg atgtcgttct tcttcacgaa gccgagcacg
     1141 tcgacgtaga acgtctcggc cttcgcctgg tcgtcgacga agacgctggc aatgtggatc
     1201 ttcagcatgg gtctctcctc atgggtcgat cggccaccgt tcgatgatcg agcgcagcgg
     1261 ccccgtgtcg aggtggtgga acttgtagcg gccttctcgc ctggtggtga ccaagcctgc
     1321 ttcctcgagc accgcgaggt gctgggagat cgcctgtcgc gacgagccca gtccgtgctt
     1381 catcgtcagg cggctgcaga tctcgaacag agtctggccg tccttgtccg tgagctcgtc
     1441 gaggatcgcc cttctcgtcg gatcgacaag agccttgaac aaggcgctca cggggagatt
     1501 tataggcaag tggcgacttg cctgtcaagg cagtaggttc ggccgacagg cggaccagca
     1561 ggaggcacgg tgctcgactg gacgaagcac gtcatctggt ggcacgtcta cccgctcggc
     1621 ttcacgggcg ccgagggctc cgctgccgac gcaccccacc cggtgccgcg gttgcgcgcg
     1681 ctggaaccgt ggctgggcta tctgctcgac ctcggctgca acggcctcgc gctcggcccc
     1741 gtgttcgcct ccggtaccca cggctacgac acgaccgacc acttccgcat cgacccgcgc
     1801 ctcggcacct ccgacgacct ccgctggttg ctcgacacgt gccgcgagcg gggtgtgcac
     1861 gtcctgctcg acggggtgtt caaccacgtc ggccgggatt tcccccagtt ccgggacgtg
     1921 ctggagcacc gaggagcgtc acgctgggcc aactggttcg acctggatct cgaggcgggc
     1981 ggacccgacg ggttcggcta ccgcgacttc gagggtcacc acgagctggt ggcactcaac
     2041 cacgccgaac cggaggtgat cgaccacatc acgaccgtga tgaaccactg gctggacgtg
     2101 ggcgcgagcg ggtggcgact cgacgcggcc tatgccatcc cgcaggatgc gctccgggcg
     2161 atcagcgacg ccgttcacgc cgaacacccc gacgcctggc ttgtcggtga ggtgatccac
     2221 ggtgactacc cctcgttcgt ccgcacgggt gggctggact cggtgaccca gtacgagctg
     2281 tggaaggcca tctggagctc gctgaacgac cgcaatctgt tcgagctggc ctgggcgctg
     2341 cagcggcacg acgcactgct ggacgatctc gtgccgttga ccttcgtggg caaccacgac
     2401 gtcacacggc tcgcgagcag gctgaccgat ccagagttac tgggtcatgc gctggccgtg
     2461 ctcttcggcg tggccggggt accggccgtg tactacgggg acgagcaggc gttccgggga
     2521 gtgaaggaag atcgggctgg gggcgacgac gcggtgcggc ccgcgttccc ggagacgacg
     2581 gacgggctcg cgccccacgg ttggccggtg taccggacgc accaggagct gatcgggatg
     2641 cgccggcgga acgcgtggct gaccggggca cgcaccgcgg tcgaccacgt ggcgaacgag
     2701 cagctcgcgt tcaccagccg aagcggcgac ggagaggtgt cggtgctgct caacctcgcg
     2761 gaccgggagc accgcttcga cccgcgtggc gatgctcagg tgcttgcgtc gagcgggtcg
     2821 ggtggcgatt cctggctgct ggcgccgcgc tcatgggcgg tcctcgcagg caacgagaag
     2881 tcggacggca cggtctcgac ggccaccgat cagttctgac ccgccgggcg gtctcctctc
     2941 cgcaagcccg gatgacggag ggacccccag tggacgcgag cccgaccttt gtgctgatcc
     3001 ccggtgccgg gggcagcgcg ttctactggc accggctggt cgaggagctg caccggcgtg
     3061 gtcgtgcggt catcgccgtc gacctgcccg caggcgacga ctccgccggg ctcgcggagt
     3121 acacggatgc ggtggtcgct gccatcggtg accgttcgcg gatcgtcctg gtggctcagt
     3181 cgctcgccgg gttcaccggg ccgctcgtgt gcgagcgggt accggtcgaa ctgctcgtgc
     3241 tgctcaacgc gatggtgccg acgccggggg aggcgccggg cgactggtgg gagaacaccg
     3301 ggcaggcgca ggcggccgcc cagctcgctg agcgcgaggg acggtcgtcc ggggacgggt
     3361 tcaacccggc cgtcgagttc ttccacgacg tgccgcccga ggtgacggcc gcggtcttcg
     3421 cgcagccgga gccggttcag tgcggcacgc cgttcgagaa gccgtggccg ctggagaggt
     3481 ggccggacgt tcccacgcgg ttcctgcagg gccgcgacga ccgcttcttt ccgctggagt
     3541 tccagcggcg ggtggtgcgg gaacgactgg gcatccctgt cgacgagatg ccgggaggtc
     3601 acctcgtcgc gctcagcagg ccccgggagc tggcggaccg gctggaggcg tacctgtcct
     3661 gacactttct cggtcgaact gtcgatcctg gcgtttcccg attgtcgttg tagcgaccgg
     3721 actaacgaag gaggatccgt gcagtacctg ctgatgatct gcggagtcga ggacgccgag
     3781 cccgtgcccg gtgtggacat ggccgccgct ggtgaggcgt gggccaagga gatggacgga
     3841 cgcggcgtcc gcctacaggg caaccggctg cgcccgattg cggacgccac gacggtgcgg
     3901 gtgcgcgaga ccgaggtgct ggtctccgac ggcccgttcg cggagaccaa ggagcagatt
     3961 ctcggctacg acctgctcga ctgccaggat ctggacgagg cgatcgaggt ggcgtcgaag
     4021 cacccggtgg cgcggtacgg gtcgatcgag gtgaggccgt tctggaccgg gtgacgggcg
     4081 tcgaggcggc ggcggtcgag gccgccgtcg tcgcggcgtt ccgcgcggag tggggccggg
     4141 tcgtcgcgac gctgatccgg atgacgggcg actgggatct cgcggaggag tgcgcccagg
     4201 aggcgctggc gcaggcggtg gcgcgctggc cccgggacgg tgtcccgcgc agccccggcg
     4261 cgtggctcac cacgacggcg cgcaaccacg cgctgaaccg cctccggcgc acggcgctgg
     4321 aggccgccaa gctgcgggag gccgcaatgg agcccatcca gggggaccag gacgacggga
     4381 gcgccgtgca ggacgaccgg ctccggctga tcttcacctg ctgccatcct gcgctcccgc
     4441 tggagggccg ggtggccctc accctgcgct ccctcgccgg cctcaccacc gcggagatcg
     4501 ctagggcgtt cctcgtgtcg gagtcgacga tggcgcagcg cctggtgcgc accaagcgga
     4561 agatccgcga ggccgggatc ccgttccgcg taccgcccga ccacctcctg cccgaccgga
     4621 cggccgccgt gctcggtgtg atctacctgc tgttcaacga ggggtatgcc gctgccgccg
     4681 gttccgagct gctgcgccac gacctgtgcg cggaggcgat ccggctcgcc cgcacgctgg
     4741 cccggctcat gcccgacgaa cccgaggtcc tcggcctggc cgccctcctg ctcctgcagg
     4801 actcgcgccg cggcgcgcgg ctggacgaca cgggcgagcc ggtcacgctg gaggaccagg
     4861 accggtcccg gtgggacgcc gccgcgatcg ccgaggggct cgagctgctc gacgccgcgc
     4921 tccggcgcgg gggtccgggc cggcggccgg cgggtcccta ccaggtgcag gcggccatcg
     4981 cggcgtgcca cgcgagggca gggcgggcgg tggacaccga ctggccgcag atcgccgcgc
     5041 tctacggcag gctcgtcgag atgacgtcct cgccggtcgt ccggctcaac cgtgctgtgg
     5101 ccgtcgccat ggccgacgga ccggcagccg ggctggagct ggtggacgcc ctcggtgggg
     5161 agccggcggg ctaccacctg ctccccgcaa cccgggccga cctgctccgg cggctcggca
     5221 ggtacggcga tgcggccgac gcctaccgcg atgcactgcg gctcaccggc accgacgccg
     5281 agcgccggtt cctcacccgc cgcctcgccg aggtcagccc gccgccaccg ctcgcctcta
     5341 ctggtacgcc tgccggtcgt ctgtaagacg cccgacctca tcgaggcgag ccgggacgcg
     5401 gccgagcgcg gcgagcgcct cgacgacgaa gtagaccccg cggaagaact gcagcacgtc
     5461 gccgcgctcg agccggaccc gccggtagat gagcatgcga cccgcgtcgg ccccgtcgag
     5521 cagctcgcgc cacgcctcgt gggtggcgcg aaggacatag gtcgcccccg cggcctcctc
     5581 cggccccacg atcgcggctc gcgtgcaccg tccccgctcc cactgcagca gcaggtgggc
     5641 cggcccaccg ccgaactccg cgggaaggtc ggggtcgacg agcgcccacg agtcgtcgta
     5701 gccggcccgg acctcgtcga tccactccca gtacgtcggg agcttctcgg cccgcagctc
     5761 gtcgctcgga ccgcggtcca ccgcatcccg cacggcttcg gcccactcag ggctgaacag
     5821 tgcaggcgcg ctggtcgtcg tcgtgggcat cgtcagcctc ccgccagctc gggctggagg
     5881 cgatcgatct gctccctgag gtcgggaggg aggtcgatgc cgacgtcgga gagcttcatc
     5941 gtcagctgcc ggatcatcag cctgcgcacc tcctccgggg tccagccgag cgactccaga
     6001 gcgagccgac ggctcacggc cccgttcatc cgcaggatcg tgcgcagcgt ctcgtggatc
     6061 tcgtggagca tggccgggtc ggactggacg gcgtcccgca ggatccgcat gcccccctgc
     6121 acatggcgcg cctcgtcccg gcaggtcgcg gtgaaccccg agtagtacgc ggggaggagc
     6181 ttgcgggtgc ggcagtagga cagcgtgatc ttcatgaccg acagcgcgag cacgccctcg
     6241 atccacagga agtaggtggt gccgtagcgg acgcgcgccg cagggtcctc cgggtcccgg
     6301 cgcagctcgt cggcctgatg ggcgagcagc ccgaacggcc ccacgaacgt ctcggagacg
     6361 tgcgggtagg aggcgtcgag cacgtcctgg atgcccgtgc cggggagcgc cacgacctcg
     6421 cggtagaagc ggtcgaaaaa gatcgtgtgc cgggcctcgt cctcgatctg gctcgacatg
     6481 tagatcttgg actcctcgct gccgccgagc atgaacaccg gcagctcgac ctcaacctgg
     6541 cgttcgccgt ggtggaagcc ggaggagatc gcgacgaacg ccgcgcgctc ctcggcgctc
     6601 atccgggtgc gccagtcgat ggcgtcctgg tcgaagtcga gctcatagac atcccagcgc
     6661 tgccggaggt agcgccggta cagatcgagg tagtcaggga gcttcttgag gtccgcgtgc
     6721 aggtagtcga gcacgtcgtc gatgtcgacg tcccggatct gggcgagggt ggagttcgcc
     6781 accctctcct gcaccgtctc ttgccgtgtc accatgccgt cgccccccgt cagcgcaccg
     6841 tgcgcggccg ccctcgaccg tcgcaggctc tagaaccgcc ttcaccgtag gtcaatcggc
     6901 gccggtccgg cactggcgag cgccgtccgg tcgcgctcca cctcggcgcg gtgctccgcg
     6961 acctcccggg cctgccgggt ctcgtcccag cccagcagcg gcgcgaggag agcggccacg
     7021 gccggggcgg ccgccgtgcc gccgtccgtg cgttcgatga ccaggtgcgt ccggcgggtg
     7081 aggacgtcgg acagggtcgc cgcgccctcg tgggtgaccg cgtagcggaa ctccacgggc
     7141 aggtagccct ccgcgccacg taaggggcgg ccgagcgccg ggtcgtcgcg aacgggcgcc
     7201 agcaccgccg gcagctcggc gccgtaacgg tgcagcatgc ggtgcacgtg gtcagcggga
     7261 acgccgtgct ccgccgccag tcgcggcacc cggttgccga ccgcgcgcca gccggcggcg
     7321 ccgaggagcg gcatctcggc ggtcggcgac ggagggagcg cacggcccag ttcgcacccg
     7381 gcggcgtcga cggcgtcgcg ggccatccgc cggtaggtcg tgtacttccc accgacgacc
     7441 gtgaccatcc cggccggctc cgcgacgacc gtgtggtcgc gggagagcgc ggacgtggtg
     7501 gcggcgtcgc gggtcaccgg cgcgagcaac gggcgcagcc cggcgaacgc accgagcaca
     7561 tccgagcggg agagcgggcg gccgaggtag cggttgacgt tgcgcagcag gtagtcgacg
     7621 tcggcctgct ctaccgtggg agcccggcga tcgcccgtgt aggtggtgtc ggtggtgccg
     7681 agcagccagt ggccgaacca cctgcgcagg atgatcacgg agtcctcggc gcgggcgagc
     7741 aacccggtgc gggagtcgaa cgcgccctgc tccaccagca ggtggacgcc cttggccggg
     7801 cgaacggcga acgaggccga tccggccatc gcctgcacgt ccgccgccca cacgccggcc
     7861 gcgttgatca ccacggacgc cgtgacgtcg aaccggtcgc cgctcagtgc atcctcgacg
     7921 acgacgcctg ccacccgtgg gccctcgcgg agcacgccga ccacccgcgc gtggctcagt
     7981 acgtgcgccc cgagtgctgc cgcggtgcgg gcgacggcga gggtgtgccg cgcgtcgtcc
     8041 atccggccgt cgtagtactg cacggcgccg gtgacgacgt cggccgccag gccgggggcc
     8101 tcccgcagcg cgccgcggcg cgtcaggtgc cggtggtgcg gtactccacc gcgcccgccg
     8161 gtgcgccctg ccgccgccat caggtcgtac aacagcacgc ccgcgccgat gtagggccgc
     8221 tcccacggcg tggtgaacgg gacgaggaag ggctcgggcg tcacgaggtg tggtgcggtg
     8281 gtgcggacca tgaggtcgcg ctcgcgcaag gcctcggcga cgagcgagaa gttcagctgc
     8341 tccaggtagc gcagcccgcc gtgcaccgtc ttgcccgacc gcgacgacgt gcccgaagcc
     8401 aggtcgcccg cctcgacgag caccaccgac aggccccggc tcgccgcgtc gagcgcggcc
     8461 ccggccccgg tcaccccgcc gccgacgacc acgacgtcga accgcccggt gcgggcggcc
     8521 gcgaccgcgg cgcgtcggcc ggcgggggac agccgcccgg gctccgacgt cccgtctgac
     8581 ggatcggctc cggcaccgct caccgggaca ccgctcaccg ggacaccgtc gccagggacg
     8641 cccgcctggt ggcccagaac ggctgcgcgg cccggacgtg gtcgaggtag cgcgcgtagc
     8701 cgtcgtcgta gtgggcctgc accgtgccgt cgggctccac gatcccctgc gccgccgtcc
     8761 acgtgccgac gtcgagcggc tcgccgcgcg cggccgccgc ggcgagcacg gcgccgcgag
     8821 cgcccacctc cggtgtgcgg gccagctcca gcggcacgcc gagaacgtcg gcgaagacct
     8881 ggagccacgg ccgtgaccgg ccgccgccgc cgcaggccac gagccgcgcc acgccacccg
     8941 ccgcctcgaa gcagtgccgt gccgcgtacg ccaggccctc gcacatcgct cgcaccagat
     9001 cgtcgcgggt ggtggtcagt cgtacgccgg tgacctgccc gcaggcctcc gggtcgacga
     9061 acggcgcccg ctccccggac gtcgcgaagt agggcagcac ctccacacca cccgcgcccg
     9121 gcgggctcgt ccccagcgcg gcgtcgatcg cctcgacgcc gagccccagt gtggtgagca
     9181 tccagtccat cgacgccgta ccgaccatcg cgggcatggc ccgcagccag cggcccggct
     9241 ccccggttgc gacggagaac ccggcgggtt cgccgtcggt cgcgagccgg tcgacgtgga
     9301 ccaggcagcc gagcgtggtg cccacgatca gcaagccgtc gccgatgccc cgcacgccgc
     9361 ccccgaacgc gcaggccggg aagtcgaacg ggccggagct caccggaacg ccaacgggaa
     9421 gtccgagcag ggcggccccg gccgcggaca gctcggcctg gggcacgggc gtggaaatcg
     9481 gcgcgagcag gttgctgcgg tgggccagcc ccatcgcgtc gaggaccgcg tcgctgtagc
     9541 cggtgcccgt gccgtcgccg aaaggtagcg agctgtcgct ggggtcggtg gcccgcacgc
     9601 cggtgagccg catgaacagg gcgtccttgc aggtgcctgc cgtggcggcc gcgtcgagcg
     9661 acgcgggttc gtgtgcgtcg agccagccga gcaggggccc gggcgcgccg gggaagagcg
     9721 tggtgccgtt ggtccggaac acccgctcgg tgacgccgtc ggccgtccag ccgccgagca
     9781 gctccgacgc ccggccgtcc agccaggaca gcgcgggtcg cacgggtgcg cctgccgcgt
     9841 cggtgagcca gaggccgtcg ccctggccgg tgagggccac cacactcggc gagcggcctg
     9901 ccgtcgcgac cacctcctgc accacttcgc cgagatgggc gagcacgtgc tcgaggtcct
     9961 gctcgacggc gccgtccgcg ccgtgcacga ggtcgagggg ccgacccgcg acggcgagcg
    10021 caccgccgtc ttcgtcgaag agggcggcct tgaccacgga ggtcccggcg tcgacgccga
    10081 gccaggtcgg ggggttcatg accgcccggg caccgactgc ccgtagctac cgagtttctc
    10141 cgccaccctg gcgatctcgt cgagctgcag caggttcggc tccccgagca accgggcctg
    10201 gtagtacatc tgacagaccc actccaggta gacgctgcgg gtgtaggcct tcgcgaggct
    10261 gtcgccgtag gtggtggcgc cgtggttctg gaggatcacg ccgaaccggc cttccatcgc
    10321 gcgcacgctg ttctcggcca gctcggagct gccatacgtg gcgtacggag caacccgcac
    10381 cgggccgccg agaagcgcca ccatgtagtg caccggcggc agctcgtcga cgaccgcgga
    10441 gagtgtgctc gcgtagagcg ggtgggtgtg caccacggcc tgcgcggagg tgtgccggta
    10501 gaccgaggtg tgcatctgca cctcggatga gggctcgagc ccgtcctcga ccggcgtccc
    10561 gtccaggtcg atcacgcaga tcgactcggg ggtgaggtcc tcgtaggccg cgccgctcgg
    10621 cgtgatcgcg atgtggtcgc cggaccggac ggagatgttg ccggaggttc cgacggtgag
    10681 cttgtcggcc tgcatccggc ggcagtactg caccagctgg acgcgttcgt cgtggaggag
    10741 catgtcgggc gggttccttc ccggtgggcg acgagggcgg cccgggctca ttcggcccgg
    10801 ccggccacct ccacacgcac gtcgttctcc cgcagcgcgg ccaggtcgcg ggcgggaacg
    10861 tcggcgtcga cgatgacgga ggcgaaccgg tgcaggggca gcaggcggtg cagtgcgctg
    10921 cgcgtgagct tgctgtggtc gatgagcagg tgggtgcgtg cggcgacctc gaccatcgcg
    10981 cgcttcaccg cgacgatctt gtcctcctgg tggaaggcgt agccctcgct caccgccgat
    11041 gtgctgacga aggcgacgtc gaaccgcatc gaacgcaccg cgtcgataca cccggagccc
    11101 aggaacgagt cgtgctgcac gtcgtagtgg ccgcccaggg ccatgacttg gacgtcgcgg
    11161 aggcgggcca gctcgacgag cgcggcgagg tagttggtgg cgaccgtgag cggtgcgagc
    11221 gcggcgaggt gggggagcat ctgcgctgcg gtggtggagt cgtcgatcag caccgacatg
    11281 cccggctcga cgtgccggca ggcgtgctgc gcgatcattc gcttggcctc gacgctggcc
    11341 ttcgcgcgga actccacgtt ggactcgaag gtgccgctgg gccgcgccgt ggcgacccca
    11401 cgcgccttac gcagcacgcc ctggcgctgc agctcgtcga gatcccggtg gatggtcatc
    11461 accgagacgc cgaacgcctc ggccagctcc tgtgcggaga ccgttccacg gcgcagcacc
    11521 aggtcggtga tgcgggcctg cctgccctcg ggaccgagcg cacgcgccgg aggagtgttc
    11581 tcggacggtt cgtccgagtg cccggccacc tccaccactg gtcttctcct tcgctcgaac
    11641 tgctgcgtct ggcatcacag cccctcgcgc ccgatcagtc aagcaaagat gtgatagcga
    11701 catagatcgg tgtgattgtc ggagatcgtc agcaacctgt gcgactccca taggctgtgg
    11761 cctgcacaaa cgctccggat cggccggaca gacatcacac acaaatctgt gacaaccacg
    11821 tattgacgtg agatgtgctc cgccatactg tggcgcctcg cggcgatccc cgtggaacga
    11881 gtcacacgcc gtgtccgagg aaggagcaac ggtgcccggt ccggagcctg ctgacgaccc
    11941 tgcagccgac gcgctgtggc tgggcgtcga tatcggcacg cagggagtcc gggccgtcct
    12001 cgtcgacggc gccggcaccg tgctcggtac gggctccgcg ccccttcatc gcgacgtgcg
    12061 cgacggcgac aggcacgagc aggaccccgc ggagtggtgg agtgccacct gcaccgccac
    12121 ccgtgccgcg ctcgccggcc ccgtgggccg ccgggtgggc gcggtgagca tcgactcgac
    12181 ctcgggcacc ctggtcgtgc agtccccgag cgggctcccg gccgggcccg gcctcatgta
    12241 tgacgaccgg cgtgccgctc tcgaggccgc ccgggtgcag gaggtgggcg atgcgctgtg
    12301 gaacgcgctc ggctaccgga tgcaggccag ctgggcgctg ccgaaggtcg tccagctcgc
    12361 cgggcggggc gcggtgcgtg ccgggcaccg gttcgcccac caggccgacc acatcgccgc
    12421 gcggctggcc gggcaccccg ttcccaccga ctggagccac gccctcaaga ccggctacga
    12481 cctgctcggc gacacctggc ccaccgcggt gctcgacgcc ctcggcgtcg acccggcgct
    12541 gctgccgccg gtggtgaccc cggggcagcg ggtggccgtc gtgtcggccg cggctgccga
    12601 ggcgtccggg atcccggccg gcacgccgat cgccgcgggc atgaccgacg gctgcgcggc
    12661 ccaggtggcg accgcggcgc tcgcgcccgg tgagtggtgt tcggccctgg gcaccaccct
    12721 cgtgctcaag ggctccacgc gtgagctcct gcgcgatccc accggcgcgg tgtactgcca
    12781 ccgcaacccc gacggcggct ggcttccggg cggcgcgtcg agcaccgggg ccggcgtcct
    12841 ctcccgcgac ctcgccggag aggacctcga cgcggtcacg gagcgcgcgc ggcaccgtgg
    12901 agttccctcg ggcgcaacgt atccgctggt gggcacgggg gagcggttcc cgttcgtcgc
    12961 ccacgacgcg gcgggcttcg acgtcggcgg ccccgtggac ggcgac
//
LOCUS       DASRSC010000256        12605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2717460,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000256 DASRSC010000000
VERSION     DASRSC010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12605
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2717460"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VFE39_12515"
     CDS             <1..230
                     /locus_tag="VFE39_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259466.1"
                     /translation="SLPSLPVVGALPSLPSLPSADSLPSLPSLPSADQSAAHSLQAAV
                     QLDLPNSSTATDTLPGAGDVFSTSDLLAGLI"
     gene            356..1480
                     /locus_tag="VFE39_12520"
     CDS             356..1480
                     /locus_tag="VFE39_12520"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET6259467.1"
                     /translation="MDVWAAAAERERTHGDLVNLSAGQPSTPAPPAVREAAVAALTDD
                     LLGYTVSLGIPELRSAVAGHYARRYDLDVPPEQVVITTGSSGGFLLAFLSAFDAGDRV
                     ALARPGYPCYRNILTALGCEVVELPCGPETRYQPTVAMLEALDEPVRGLVVASPANPT
                     GTVLDPTELVALASYCERNGVQLVSDEIYHGISYAGAPATSSAWSTSSEAIVVNSFSK
                     YFSMTGWRLGWLLLPPRLLRPAECLAGNFTVCPPALAQRAAIGAFDPTSYVEADSHVA
                     RYAVNRGLLLDGLPRLGITRLAPADGAFYVYADVSHLTGSKTSMQWVYQALSEVGVAL
                     APGIDFDPVDGDRFVRLSFAGATDDITEALRRLAGWLRGS"
     gene            complement(1639..2775)
                     /locus_tag="VFE39_12525"
     CDS             complement(1639..2775)
                     /locus_tag="VFE39_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HET6259468.1"
                     /translation="MRSTSRARRPAAHDGPIDLRSDTVTLPTKQMRAAMAAAEVGDDV
                     LDRDPTMRELEVRVAGLLGAADALWTPSGSMGNLIALMHHLDRGDAFLAPAGAHVLDA
                     ELGTAAWLAGGMPRPMDHDAGPGKVSPAAVRRAVGSTGPYYSLRTTLLCLENTHNAAG
                     GAITAPEEHAAVVAAARGAKLRVHLDGARLWHAAVALGVPPGALTVGADTVQVCLSKG
                     LGAPVGSVVAGSAEFVEEARRLRKMLGGGVRQGGVLAAAGLVALDRVDRLSEDHERAR
                     TLAAGLRERGWLVGVPETNIVLVAVADLDGTIRRFADAGVRASTMAGQVRLMTHADLT
                     DADITAALERIGPVEPSRPGATAPAPGRAARLRAGRAPAHPAGC"
     gene            2936..3466
                     /locus_tag="VFE39_12530"
     CDS             2936..3466
                     /locus_tag="VFE39_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HET6259469.1"
                     /translation="MDEAGEREFVPDTFEEYFRARRDSVRRTAFLLCGDWHRADDHAQ
                     AAFVALHRHWRRIRDRGALDAWMRRTLVRAVVDESRRPWRRERFTPEPAHASSVPSDS
                     DAVATRHVLVDGLRSVPPRQRAVLVLRFLDGLDVAATATALGCSEGTVKSQTAHGLAA
                     LRTVLGDALDDLRPAG"
     gene            3482..4180
                     /locus_tag="VFE39_12535"
     CDS             3482..4180
                     /locus_tag="VFE39_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259470.1"
                     /translation="MTDVDDEELGTLFRAAASDSGAPPPRFDHDSVVSASRRAAARRR
                     AALAGGVVALFAVVGVVATIALPASPDRDASTVAAPMLAPNPGARLDAGPAADAGSGQ
                     AAAEAAGAGAPPLGPGTAECADRQDPALRTIVEEVLPGVAGAPEAATTMECRPRGERG
                     INVEVSDGGAVGLLSVQYLPPGVIVALVPGAVSAPTVSGGTVIVSSRASEPGAPPPFA
                     DRLESTAAFLAPRL"
     gene            4240..4818
                     /locus_tag="VFE39_12540"
     CDS             4240..4818
                     /locus_tag="VFE39_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET6259471.1"
                     /translation="MGTAKGERRRQELVTAAAALLRSGGFDAVRHRAVAERAGLPLAS
                     TTYYFASLDDLVTAAVERTSRDELAEGREQLDALADGPVATPALVELVLDQLLGPESR
                     DGGLDAVLLRYERLVGAGRRPYLAPLMRELREDLDALLAEILVRSGHPLDAAALRDLV
                     ALVDGAVISALIEADADPRSVASGVMIRALRA"
     gene            4899..6317
                     /gene="purB"
                     /locus_tag="VFE39_12545"
     CDS             4899..6317
                     /gene="purB"
                     /locus_tag="VFE39_12545"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET6259472.1"
                     /translation="MIPNVLAARYASPELVRLWSPEHKIVLERRLWIAVLAAQRDLGV
                     DVPESAIADYERVVEQVDLESIAARERVTRHDVKARIEEFDALAGHEHVHKGLTSRDL
                     TENVEQLQVRLSLEHVRDRTVAVLSRLARLAAEYAELVMAGRSHNVAAQVTTLGKRFA
                     SAADELLVAYGRLEDLRARYPLRGIKGPMGTAQDMLDLLDGDAAKLASLEDRVAAHLG
                     FAGVLTSVGQVYPRSLDFDVLSALLQLAAGPSSLATTIRLMAGIELATEGFQPGQVGS
                     SAMPHKMNSRSTERINGMMVLLRGYAGMAAELAGAQWNEGDVSDSVVRRVALPDAFFA
                     LDGLFETTLTVLDEFGAYPAVIARELDRYLPFLATTAVLMAAVRAGVGRETAHEVIKE
                     HAVGVALAMREKGQADNDLVARLAADERLGLPAGALDGLLADPLRFTGAATTQVSAVV
                     ARVREIVDAHPEAAAYTPGAIL"
     gene            6314..7144
                     /locus_tag="VFE39_12550"
     CDS             6314..7144
                     /locus_tag="VFE39_12550"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET6259473.1"
                     /translation="MNLLHSGKVRDVYADGDDLILVASDRLSIYDVVLPTPVPDKGAL
                     LTQLSLWWFDRLADITAHHVISASDVPAEFVGRAVRCRRLSMLPVECIARGYLAGLGL
                     KEYEKQGTVSGVALPPGLVEGSKLPEPIFTPTTKAPVGEHDEFMTFDEVVDVVGRDTA
                     ARLRDLTLAVYARGAEIAARNGILVADTKLEFGLDPAGEIVLADEVLTPDSSRFWPAD
                     TWEPGRPQFSFDKQYVREWSATMNWDRRPPGPEVPTEVVAATRARYVEVYERITGEEW
                     "
     gene            7218..8009
                     /locus_tag="VFE39_12555"
     CDS             7218..8009
                     /locus_tag="VFE39_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003095964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2334 domain-containing protein"
                     /protein_id="HET6259474.1"
                     /translation="MVTQPPVAHLVVSLSGLADDSPESLSRGADFTAELDERGVPLSQ
                     LFRPRAASGTLRVDSPLVRWMHARRAAGDAVVLHGYDHTADPIGSWQSSALARVGRRA
                     EFATLPRHEAGLRLTAARRALTVLGLRTDLFVPPRWLASPGTVEALHDQGFGVLADEN
                     GVRLLQGQGIDTVVRARVLGFRVSGERHPLADDRKAAEAWRCRMLEAEVARTVRRGGL
                     VRINVRAKDLKRGARRTAVLAAVDAALRIGAVPATYRLTATSRAA"
     gene            8312..8938
                     /locus_tag="VFE39_12560"
     CDS             8312..8938
                     /locus_tag="VFE39_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6259475.1"
                     /translation="MQTTHFGHSCLLLDTGAARLLFDPGTFSTGFEAVDGLDAVLVTH
                     QHFDHIDADRLGALLAANPDASLIVDSGTAEQLADIEHEVVAPGASIQVGGSRIDVLG
                     GEHAVIHPDIPVIANNAYLIDGTHLHPGDSFTPPPAPVDVLFLPTGAPWLKLSEAVDY
                     MRAVAPRTAVPIHEAVLARPAMHYRLFEQLAPEQTTVRVLDRETATAL"
     gene            complement(9229..10224)
                     /locus_tag="VFE39_12565"
     CDS             complement(9229..10224)
                     /locus_tag="VFE39_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HET6259476.1"
                     /translation="MGRWLVLGGTRFLSRAVAAAAVARGHEVVCVARGVSGGVPDGTK
                     LLVADRDVPGALEPLRGEPFDAVVDVARMSLPWVRSALHALAVNAAHWTFISTINVYA
                     DSTTPDQGVDATLVVPRTDDTPTEDPEAYGPIKVASENAVRDTVGPRALIVRSGLICG
                     PDDGSDRFGYWPNRFARGGHAVVPADPGQPAQLVDVRDLADWIVRSGEEHVVGTFDGS
                     GPRSTLGAVLDEIADSVGAAGLEQVAVAPEALTAAGVQPWSGPRSLPLWLTGSDVGIL
                     ARNTAPAAAAGLRCRPVAETAGAALEHERALGPDRPRRAGLTPAEESEILAAHLP"
     gene            10285..10518
                     /gene="purS"
                     /locus_tag="VFE39_12570"
     CDS             10285..10518
                     /gene="purS"
                     /locus_tag="VFE39_12570"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HET6259477.1"
                     /translation="MAKVVVDVMPKPEILDPQGQAVAGALARLGVAGVEDVRQGKHFE
                     LEVDDSVDDETLHRVAGSLLANPVIEDWVVTRT"
     gene            10515..11189
                     /gene="purQ"
                     /locus_tag="VFE39_12575"
     CDS             10515..11189
                     /gene="purQ"
                     /locus_tag="VFE39_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HET6259478.1"
                     /translation="MRIGVITFPGTLDDVDAARAVRYAGAEPVALWHADRDLQGVDAV
                     IVPGGFSYGDYLRAGAIASLAPIMTEVVDAAGRGTPVLCICNGFQILCEAGLLPGALT
                     RNSGLHFVCRDQRLLVESSSTAWTSDLTAGQEILVPLKSGEGRFVADAATIERLEGDG
                     RVVFRYAGANPNGAMSAIAGISSADGRVVGLMPHPEHAIDALTGPSRDGLALFTSVLQ
                     IAASAV"
     gene            11285..12523
                     /locus_tag="VFE39_12580"
     CDS             11285..12523
                     /locus_tag="VFE39_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HET6259479.1"
                     /translation="MTRSVSAGPRHVLLVEDDAGDVFLVRELLAEVDPGLRVTVAESV
                     SDVVERGLLAHCDCVLLDLNLPGSDGLDGLLTILQTGGSPAVCVLTGLDDEHLGTAAV
                     AAGAQDYLVKAKVDGQVLIRAVRYAVERRRAESSLLRLREEQLAAAESARLERGLLPS
                     PLLAGSPVRARSFYRAGRTRSLIGGDFFDAVLGPDGTVHAIVGDVCGHGPDEAALGAL
                     LRVSWRALVLAGVDEPQVLPKLQQLLITERHVRSLFTTVCTVAFRSELDAAGVRSATE
                     MLVRLAGHPPPVSLIPVPRPAAPPVGLPLGVAADATWEPVCVPLGAEWALLLHTDGLI
                     EGRGPVPGEPLWEEGLLEMLEQERDTDLEKLPARLVERAQDLNGGPLSDDIAILLLSS
                     DTAPQDAPQAGMAPSAVELP"
BASE COUNT         1634 a         4565 c         4570 g         1836 t
ORIGIN      
        1 actccctgcc gtcgctgccg gtcgtcggcg ccctgccgtc gctgccctcg ctgcccagcg
       61 ccgactcgct tccgtcgttg ccgtcgctgc cgagcgccga ccagtccgcc gcgcacagcc
      121 tgcaggcagc cgtccagctc gacctgccca acagcagcac cgccaccgac accctccccg
      181 gcgccggtga cgtcttctcg acctcggacc tgctcgcagg cctgatctga ggcgaccggc
      241 ggccgtcctc ctcgggtggg gacggccgcc ggcccgcgct taggctcgag gcgtgcaccc
      301 tctgacatcc gggatcacgg ctgcggcccg tgcctcgatt cctcccttcc acgtcatgga
      361 cgtgtgggca gccgctgccg agcgagagcg cacccacggc gacctggtga acctctccgc
      421 gggtcaaccg tccacaccgg ctccccccgc cgtgcgggag gccgcagtcg ccgcgctgac
      481 cgatgacctg ctcggttaca cggtctcact cggaattccg gagctgcgtt ccgctgtcgc
      541 cgggcactac gcccgccgct acgacctcga cgtgccgccg gaacaggtgg tgatcaccac
      601 cgggtcgtcc ggcggcttcc tgctcgcgtt cctgtccgcg ttcgacgcgg gggaccgggt
      661 ggcgctggcc aggcccgggt acccctgcta tcgcaacatc ctgacggccc tcggctgtga
      721 ggtcgtcgaa ctcccgtgtg gccccgagac ccgctaccag ccgaccgtcg cgatgctcga
      781 ggcgttggac gagccggtgc gagggctggt cgtggccagc ccggccaacc cgacggggac
      841 cgtcctggac ccgaccgaac tcgtggctct cgcgtcgtac tgcgagcgca acggcgtcca
      901 actggtgagc gacgagatct accacggcat ctcctacgcg ggcgccccgg cgacctcttc
      961 ggcgtggagc acctcatccg aggcgatcgt cgtcaactcc ttctcgaagt acttctccat
     1021 gaccgggtgg cggttggggt ggctgctgct gcccccgcgg ctgctgcgcc cggccgaatg
     1081 cctcgccggc aacttcaccg tctgcccgcc cgctctcgcg cagcgggccg cgatcggcgc
     1141 gttcgatccc accagctacg tagaggccga ctcgcacgtc gcccggtacg cggtgaatag
     1201 gggactactg ctcgacggcc tgccacgcct gggtatcacg cgcctcgccc cggccgacgg
     1261 cgcgttctac gtctacgcgg acgtctccca cctaacgggt tctaagacct cgatgcagtg
     1321 ggtctaccag gcactttccg aggttggggt agctctggca cccgggatcg acttcgaccc
     1381 cgtcgacggg gaccgcttcg tacggctctc gttcgcgggc gctacggacg acatcaccga
     1441 ggcactacgg cgcctagccg ggtggctacg ggggagctga gcggagcgtc ttctacgtct
     1501 ggtccgcgcg tcacaccaga cctcttgtag gccagcgcgg tggatccggt tgtcctgcgc
     1561 gggggacacc gacgccgtca cggaagcgct gcggaggatg gggactggct cggctcggct
     1621 gaagcggtca ccgcgtgatc agcaccccgc gggatgcgct ggggcgcgac cggcccgcaa
     1681 cctggccgcg cggcccggcg cgggcgctgt ggcgcccggc cgggacggct ccaccggccc
     1741 gatccgctcc agcgccgccg tgatatcggc gtcggtcagg tcggcgtggg tcatgagccg
     1801 gacctgccct gccatcgtcg acgcgcggac gccggcgtcg gcgaagcggc gaatcgtgcc
     1861 gtccaggtcg gccacggcca ccagcacgat gttggtctcc ggcaccccga ccaaccagcc
     1921 gcgctcgcgc agccctgcgg cgagcgtcct ggcgcgctcg tggtcctcgg acaggcgatc
     1981 cacgcggtcc agcgccacca gtcccgccgc ggcgagcacc ccgccctgac ggacgccgcc
     2041 gccgagcatc ttgcgcagcc gcctggcctc ctcgacgaac tcggccgagc cggcgaccac
     2101 tgacccgacg ggcgccccga gacccttgct caggcagacc tgcacggtgt cggcgcccac
     2161 ggtcaaggcc cccggcggca cgcccagcgc caccgcggcg tgccagagtc gggcgccgtc
     2221 gaggtgcacc cgcagcttcg ccccccgcgc cgcggcgacg acggccgcgt gctcctccgg
     2281 tgcggtgatg gcgccgcccg cagcgttgtg cgtgttctcc aggcacagca gcgtggtgcg
     2341 cagcgagtaa tagggcccgg tggagcccac ggcgcggcgc acggctgcgg gcgagacctt
     2401 gccgggaccg gcgtcgtggt ccatcggtcg cggcatcccg ccggcgagcc aggcggccgt
     2461 accgagctcg gcgtcgagca cgtgcgcgcc cgcgggagca agaaacgcgt cgccgcggtc
     2521 gaggtggtgc atcagcgcga tgaggttgcc catcgagccg ctgggggtcc acagcgcgtc
     2581 ggccgctccg agcaggcctg ccacccgcac ctcgagctcg cgcatcgtcg gatcgcggtc
     2641 gaggacgtcg tcgccgacct cggcggccgc cattgcggcc cgcatctgct tcgtggggag
     2701 ggtgacggtg tccgagcgga gatcgatcgg gccgtcatgg gccgccggcc gccgggctcg
     2761 tgatgtggat cgcacggcat gcgattcaac caggaggcga cgggctcgtc tctgctgggt
     2821 cacagaactg tcacgacgtt gggctattcg tgtgaagcgc gcccaactgg gcaaccccgg
     2881 agcccgatcc ttgcgtcccg tatccgacac cgacgtggga ggggggaaca gccgggtgga
     2941 cgaggccggc gagcgcgagt tcgtcccaga cacgttcgag gagtacttcc gcgcacgtcg
     3001 ggactcggtg cggcgtaccg cgttcctgct gtgcggcgac tggcaccgcg ccgacgacca
     3061 cgcacaggcg gcgttcgtcg cgctgcaccg gcactggcgg cggatccgtg accgcggtgc
     3121 gctcgacgcg tggatgcggc gcacgctcgt gcgcgccgtc gtcgacgagt cccggcggcc
     3181 gtggcgcagg gagcgtttca cccccgagcc cgcccacgcg agcagcgtcc cgtccgactc
     3241 ggacgcggtc gccacgcggc atgtattggt ggacgggttg cggtcggtgc cgccgcgcca
     3301 gcgtgccgtt ctcgtgctgc ggttcctcga cggcctggac gtcgccgcca ccgcgactgc
     3361 gttgggttgc tccgagggca cggtcaagag ccagaccgcc cacggcctcg cggcgttgcg
     3421 cacggtgctc ggtgacgcgc tcgacgacct acggcctgcg ggataggaga gaggtgacgg
     3481 gatgactgac gtcgacgacg aggagttggg cacgctgttc cgtgccgccg cctccgactc
     3541 gggggcgccg ccaccgcgct tcgaccacga ctcggtggtg agcgcgtccc gtcgcgccgc
     3601 tgcacgtcgc cgtgctgcct tggcgggggg cgtggtcgcg ctgttcgcgg tggtcggcgt
     3661 cgtggccacg atcgcgctgc ccgcctcccc cgaccgagac gcctccacgg tcgcggcgcc
     3721 gatgctcgcg ccgaaccccg gcgcgcggct ggacgccggg ccggcggcag acgccgggag
     3781 cggccaggct gcggcggagg cggcaggagc gggagccccc ccactcgggc cgggcaccgc
     3841 ggagtgcgcc gatcggcagg accctgcgct gcggacgatc gtcgaggagg tcctgcccgg
     3901 cgtggcaggc gccccggagg cggccaccac gatggagtgc cgtccgcgcg gtgagcgcgg
     3961 catcaacgtc gaggtcagcg acggcggcgc ggtggggctg ctcagcgtgc agtacctgcc
     4021 gccgggtgtg atcgtcgcac tcgtcccggg cgccgtcagt gcgccgaccg tctcgggcgg
     4081 cacggtgatc gtgagctcgc gcgcgtccga gccgggtgcc ccgccgccgt tcgcggacag
     4141 gctggagtca acggcggcct tcctggcgcc ccgcctctag cgccttcccg acctcgcccc
     4201 cacgctggcg gtttcgcgca gcggcggaag actgcagccg tgggtaccgc caagggtgag
     4261 cggcgtcggc aggagttggt gacggcggcc gcggcgctgc tgcgctccgg cggcttcgac
     4321 gccgttcggc accgggccgt cgccgagcgc gccgggctgc cgctggcgtc caccacctac
     4381 tacttcgcct cgctcgacga cctcgtcacc gcggccgtgg agcgcaccag ccgggacgag
     4441 ctcgccgagg gcagggagca gctcgatgcg ctcgcggacg gaccggtcgc cacgcccgcg
     4501 ctcgtcgagc tggtgctcga ccagctgctc ggccctgagt cgcgcgatgg cgggctcgac
     4561 gccgtcctgc tgcgctatga gcggctcgtc ggcgcgggca ggcggccgta cctggcgccg
     4621 ctcatgcgcg agctgcgcga ggacctggac gcgctgctcg ccgagatcct cgtgcggtcc
     4681 ggccacccgc tcgacgccgc ggcgttgcgc gacctcgtcg ccctggttga cggcgcggtg
     4741 atcagcgcgc tcatcgaggc ggacgccgac ccgcgttcgg tcgctagcgg cgtgatgatc
     4801 cgcgccctgc gcgcgtgagg gctgtgagcg gagcggcggg tggtggttcg gagcactagt
     4861 ttcgatgcac gagcgcagag cgcctatcgt ttccgcacgt gattcccaac gtgctggctg
     4921 ctcgctacgc gagcccggag ctcgtccggc tgtggtcgcc cgagcacaag atcgtgctgg
     4981 aacgccgact ctggatcgcc gtgctcgcgg cgcagcgcga cctgggcgtc gacgtccccg
     5041 agtccgcgat cgccgactac gaacgcgtgg tcgagcaggt cgacctggag tcgatcgccg
     5101 cccgtgagcg agtcacccgc cacgacgtga aggcccggat cgaggagttc gacgccctcg
     5161 ccggtcacga gcacgtgcac aagggcctca ccagccgcga tctcactgag aacgtcgagc
     5221 agctgcaggt ccggctctcg ctcgaacacg tccgcgaccg caccgtggcc gtcctctccc
     5281 ggctcgcccg gctggcagcc gagtacgccg agctggtgat ggcgggacgc agccacaacg
     5341 tcgcggcgca ggtcacgacg ctcggcaagc ggttcgcctc cgctgcggac gagctgctcg
     5401 tcgcgtacgg ccggctcgag gacctgcgcg cgcgctaccc gttgcgcggc atcaagggcc
     5461 cgatgggcac cgcccaggac atgctcgacc tgctcgacgg ggacgctgcc aagctcgcgt
     5521 cgctggagga ccgggtcgcg gcgcacctgg gattcgccgg cgtgctcacc agcgtcgggc
     5581 aggtctaccc gcgctccctg gacttcgacg tgctcagcgc gctgctccag ctcgccgccg
     5641 ggccgtcgtc gctggccacg acgatcaggt tgatggcagg catcgagctc gccaccgagg
     5701 gcttccagcc cggccaggtc ggctcgtcgg cgatgccgca caagatgaac agccgttcca
     5761 ccgaacggat caacggcatg atggtgctgc tccgcggcta cgccggcatg gctgccgagc
     5821 tggccggagc gcagtggaac gagggtgacg tctccgactc cgtggtccgc cgagtcgccc
     5881 tgcccgacgc gttcttcgcg ctcgacggcc tgttcgagac caccctgacc gtgctcgatg
     5941 agttcggcgc ctacccggcc gtcatcgcgc gcgagctgga ccgttacctc ccgttcctgg
     6001 cgacgacggc tgtgctgatg gctgcggtcc gcgccggcgt cgggcgggag accgcccacg
     6061 aggtgatcaa ggagcacgct gtcggcgtcg cgctcgcgat gcgggagaag ggccaggccg
     6121 acaacgacct cgtcgcgaga ctggccgctg acgaacggct ggggctgccg gctggggcgc
     6181 tcgacgggct ccttgccgac ccgctgcggt tcaccggcgc cgccacgacc caggtcagtg
     6241 cagtcgtcgc ccgggtgcgg gagatcgtcg acgcccatcc cgaggctgcc gcctacaccc
     6301 ccggggcgat cctgtgaatc tgctgcacag cggcaaggtc cgggacgtct atgccgatgg
     6361 cgacgacctg atcctcgtgg cgtccgaccg actctccatc tacgacgtgg tgctcccgac
     6421 gccggtgccg gacaagggcg cgctgctcac ccagctgtcg ctctggtggt tcgaccggct
     6481 cgccgacatc acggcgcacc atgtcatctc ggcgtcggac gtgcccgcgg agttcgtcgg
     6541 ccgggccgtc cggtgccgca ggctctcgat gctgcccgtc gagtgcatcg cccgcggcta
     6601 cctcgccggg ctggggctca aggagtacga gaagcagggc acggtctccg gggtggcact
     6661 gccgcccggg ctcgtcgagg gctcgaagct gccggaaccg atcttcacgc ccacgacgaa
     6721 ggctcccgtg ggtgagcacg acgagttcat gaccttcgac gaggtcgtcg acgtggtcgg
     6781 gcgggatacg gcggcacggc tgcgcgacct gacgctcgcg gtgtacgcgc gcggggcgga
     6841 gatcgcggcg cgcaacggca ttctggtcgc cgacacgaag ctggagttcg ggctcgaccc
     6901 ggcgggggag atcgtgctcg ccgacgaggt gctcacgccc gactcgtcca ggttctggcc
     6961 cgccgacacc tgggagccgg gccgcccgca gttctccttc gacaagcagt acgtccgcga
     7021 gtggtcggcc accatgaact gggaccggcg gccgcccgga cccgaggtgc ccaccgaggt
     7081 cgtcgccgca acacgtgccc gttatgttga ggtctacgag cggatcaccg gcgaagaatg
     7141 gtgatgggcg ccggagtagc acccgcccct cacagcacgg tcaacccccg ttcacgtagg
     7201 catgcgttca tgggtgggtg gtcacgcagc cgcccgtcgc ccatctcgtc gtgtcgctgt
     7261 ccgggctggc cgacgactca cccgagtcgc tgagccgcgg cgctgacttc accgcggagc
     7321 tcgacgagcg cggcgtgccg ctgtcgcagc tgttccgccc ccgcgcggcg tccgggacgc
     7381 tgcgggtgga ttcgccgctc gtgcgatgga tgcacgcgcg gcgcgcggcg ggcgacgccg
     7441 tcgtgctgca cggctacgac cacaccgccg acccgatcgg ctcatggcag tcctcggcgc
     7501 tcgcgcgggt gggtcggcga gcagagttcg ccacgctgcc ccgccacgag gccggcctgc
     7561 ggctcaccgc ggcccgccgc gcgctgacgg tgctgggcct gcgcaccgac ctgttcgtcc
     7621 cgccacgctg gctcgcgtcg cccggcaccg tggaggcgct gcacgaccag ggcttcggtg
     7681 tgctggccga cgagaacggt gtgcgcctcc tgcagggcca gggcatcgac accgtcgtgc
     7741 gggcgcgggt gctcgggttc cgcgtctcgg gggagcgcca tccgctcgcc gacgaccgga
     7801 aggccgccga ggcgtggcgg tgccggatgc tcgaggccga ggtcgcccgt accgtgcggc
     7861 gtggtgggct ggtgcggatc aacgtcaggg ccaaggacct gaaacgggga gcgcgccgca
     7921 ccgccgtgct ggcggccgtg gacgccgcgc tccgcatagg tgctgttccc gcgacctacc
     7981 gcctgacggc aacgtcccgc gcggcctgac ccgcccgcga gtcgcccggt ttcggcctgc
     8041 gagtcgcccg tccccactcc gcgagtcgcc cgatccccgc ccgcgagtcg cccgtcccca
     8101 gcccccgtgt cgcccggttt cggcctgcga gtcgccgtgg ccgggaagaa agacccctcc
     8161 gtcccgaacc tcagccacgt ctgccccgcc cggttgagca gaaggtcggc gagatgcgca
     8221 ccgagcgcga gcagcggctc aatgtccccg cgccgcgcga tctgcactgt catccgcacc
     8281 tacgcgtcct gccggtggga cactcgaaga cgtgcagacc acccacttcg gccactcgtg
     8341 tctcctgctc gacaccggcg ccgcgcggct gctgttcgac cccgggacgt tctcgaccgg
     8401 cttcgaggcg gtggacggac tcgacgccgt cctcgtcaca catcagcact tcgaccacat
     8461 cgacgccgac cggctcggcg cgctactggc cgccaacccc gatgccagcc tgatcgtcga
     8521 ctccgggaca gccgagcagc tcgcggatat cgagcacgag gtggtggcgc caggggcgtc
     8581 gatccaggtg ggcggttccc ggatcgacgt gctcggcggg gagcacgcgg tgatccaccc
     8641 ggacatcccc gtcatcgcca acaacgccta tctgatcgac ggcacccacc tgcaccctgg
     8701 cgactccttc acgccgccgc ccgcgccggt cgacgtgctc ttcctgccga ccggggcgcc
     8761 ctggctcaag ctgtccgagg ccgtggacta catgcgggcg gttgcgccgc gcacggccgt
     8821 gccgatccac gaagccgtcc tcgcccgacc ggcgatgcac tacaggctgt tcgagcaact
     8881 cgccccagag cagaccaccg tccgcgtcct cgaccgcgag acggccactg cgctctgaca
     8941 cgcccacagc tcggctgctg gcgactcgtg catgcagaca ggaattggcg ggcggagaac
     9001 aagcgactcg cgggcacgac gggggcgact cgcggacccg aacccgacca cgtgcgcgca
     9061 ccagtccgcg ggtgcggcac cacagcgact cgcacacaca tccgaggttg tgactcgcgg
     9121 gccgggggcg actcgtgggc ggaatttggg cgactcgcgg ggttggaacg gggcgactcg
     9181 tgggcggaat tcgggcgact cgcggggttg ggacgggcga ctcgcggctc aggggaggtg
     9241 cgcggccagg atctccgact cctcggccgg ggtgagcccg gcgcggcgcg gacggtccgg
     9301 gccgagagcg cgttcgtgct ccagcgcggc gccagcagtc tcggcgaccg gacggcaccg
     9361 caggcctgcg gctgcggcgg gtgcggtgtt cctcgccagg atgccgacgt ccgacccggt
     9421 cagccacagc ggcagtgacc ggggcccgga ccacggctgc actccggctg ccgtgagcgc
     9481 ctccggtgcg acggccacct gctccagtcc cgcagccccc accgagtcgg cgatctcgtc
     9541 gagtacggcg cccagggtcg agcgggggcc actgccgtcg aaggtcccga cgacgtgctc
     9601 ctccccggac cgcacgatcc agtcggcgag gtcgcgtacg tcgacgagct gagccggctg
     9661 tcctggatcg gccggcacga ccgcgtgccc gccccgggcg aaccggttcg gccagtagcc
     9721 gaaccggtcg ctgccatcgt ccgggccgca gatgaggccg gagcgcacga tcagcgcccg
     9781 gggtcccacg gtgtcgcgca ccgcgttctc ggacgccacc ttgatcgggc cgtacgcctc
     9841 cgggtcctcg gtgggtgtgt cgtccgtccg cgggacgacg agtgtggcgt cgacgccctg
     9901 gtcgggggtg gtgctgtcgg cgtacacgtt gatcgtcgag atgaaggtcc agtgcgccgc
     9961 gttcacggcc agggcgtgga gtgccgaccg cacccacggc agcgacatcc tggcgacgtc
    10021 cacgacggcg tcgaacggct cgccgcgcag gggttcgagc gcgccgggta catcgcggtc
    10081 cgcgacgagg agcttcgtac cgtccggaac gccaccggaa accccccggg ccacgcacac
    10141 cacctcatgg cctcgtgcca cagccgcggc tgccaccgcc cgggagagga aacgtgtgcc
    10201 gccgagcacc aaccaccgac ccatgggagg aggctcacgg acggtggcgc ggggcggcaa
    10261 tggtgttcgc cgtacgctga tgtggtggcg aaggtcgtgg tggatgtgat gccgaagccg
    10321 gagatcctcg acccgcaggg ccaggccgtc gcgggtgcgc tcgcgcggct cggcgtggcc
    10381 ggtgtcgagg acgtccggca gggcaagcac ttcgagctcg aggtggacga ctcggtcgac
    10441 gacgagaccc tgcaccgggt ggccggcagc ctgctggcca acccggtgat cgaggactgg
    10501 gtggtgacca ggacatgagg atcggggtca tcactttccc gggcacgctc gacgacgtcg
    10561 acgctgcccg ggcggtgcgc tacgcgggcg ccgagcccgt cgcgctctgg cacgccgacc
    10621 gcgacctgca gggcgtcgac gcggtgatcg tccccggtgg cttctcctac ggcgactacc
    10681 tgcgcgccgg agccattgcc agcctcgccc cgatcatgac cgaggtcgtg gacgccgccg
    10741 gccgcggcac gcccgtgctg tgtatctgca acggcttcca gatcctgtgc gaggccgggc
    10801 tgcttcccgg cgcgctgacc cgcaactccg ggctgcactt cgtgtgccgc gaccagcggc
    10861 tgctggtgga gtccagctcc accgcctgga cgagcgacct cacggcgggc caggagatcc
    10921 tcgttccgct gaagtcgggc gaaggtcgct tcgtcgccga cgccgccacg atcgaacggc
    10981 tcgagggcga cggccgggtc gtgttccggt acgcgggcgc gaaccccaac ggggcgatgt
    11041 cggcgatcgc cgggatcagc tcggccgacg gccgggtcgt cgggctcatg ccgcaccccg
    11101 agcacgcgat cgacgcgctc accgggccgt cgcgcgacgg gttggcgttg ttcacgtctg
    11161 tgctgcagat cgcagcgtcg gctgtgtgac agcgccccgt ggtgggtgac gtgagtttcg
    11221 ggatctagga tttcgggtgc ctcgtgcacc gtgctgcggg ggcccgcgag gagaagcgcc
    11281 cggtatgacc cgatccgttt cggctgggcc ccggcacgtg ctgctggtgg aggacgacgc
    11341 cggcgatgtg ttcctcgtgc gtgagcttct cgccgaggtc gaccccgggc tgagggtgac
    11401 ggtcgccgag tccgtgagcg acgtggtcga acgcggcctg ctcgcgcact gcgactgcgt
    11461 gctgctcgac ctcaacctcc ccggttccga cggtctggac gggctgctca cgatcctgca
    11521 gaccgggggc tctcccgccg tctgcgtgct cacgggcctg gacgacgagc acctcggtac
    11581 ggcggctgtg gcggcgggcg cgcaggacta cctggtgaag gccaaggtcg acgggcaggt
    11641 gctgatccgg gcagtgcgct acgccgtgga gcggcgccgc gcggagtcga gcctgctgcg
    11701 gctgcgggag gagcagctcg cggcggcgga gtcggcccgg ctcgagcgcg ggctgctgcc
    11761 gtcgccgctc ttggccggga gtccggtccg ggcgcggtcg ttttaccggg cgggccgcac
    11821 ccgttcgttg atcggcggcg acttcttcga cgcggtgctc gggcccgacg ggaccgtgca
    11881 cgcgatcgtc ggtgacgtgt gcggccacgg cccggacgag gcggccctgg gggcgctgct
    11941 gcgggtgtcg tggcgcgcgc tcgtgctggc cggcgtggac gagccgcagg tgctgcccaa
    12001 gctccagcag ttgctgatca ccgagcggca cgtccggtcg ctgttcacga cggtctgcac
    12061 cgtggcgttc cggtcggagc tggacgctgc cggcgtgagg tcggcgacgg agatgctcgt
    12121 gcggctggcg ggacatccgc caccggtgtc gctgatccct gtgccgcgcc cggcggcgcc
    12181 gccggtgggg ctgcccctcg gcgtggccgc cgatgcgaca tgggagcccg tgtgcgtacc
    12241 gctgggcgcg gagtgggcgc tgctgctgca caccgacgga ctgatcgagg gccggggtcc
    12301 cgttcccggt gagccgctgt gggaggaggg gctgctcgag atgctggagc aggagcgcga
    12361 caccgacctg gagaagctgc cggcccggct ggtcgagcgc gcgcaggacc tcaacggcgg
    12421 cccgctctcc gacgacatcg cgatcctgct gctctcgtcg gacaccgcgc cgcaggacgc
    12481 cccccaggcc gggatggcac cgtccgcggt ggagctgccg tgaccgctct cgacctgacg
    12541 ccgcccgagc accgcgcgga ggggcgccta cagcggttgg aggaggggag gcggcactgg
    12601 acgct
//
LOCUS       DASRSC010000257         7465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1575441,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000257 DASRSC010000000
VERSION     DASRSC010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1575441"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..1922)
                     /locus_tag="VFE39_12585"
     CDS             complement(105..1922)
                     /locus_tag="VFE39_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET6259480.1"
                     /translation="MAPRGATQRSAQNLVNLESVTAHVPGDASRVLLDAVSLGIERSE
                     RVGVVGLNGGGKTTLLDIITGDRAPDGGRVSRVGDLNLAHLAQGDTLPPGARVRDVVL
                     AAWHGAADHEWAADAKVRDVLDGLGIGDLERSVEGLSGGEKRRVALAAALVGDPDLVV
                     LDEPTNHLDVEGIGWLAEHLLSRRCGVVVVTHDRWFLDAVCTRTWEVANGRVESYLGG
                     YADWVYARAERSRQADAAEARRQNLARKELAWLRRGAPARTSKPRFRIEAAEALIADV
                     PPPRNTVELLGFATNRLGRTVLELEDATAEIAGRTLLNHVTWRLGPGDRVGIVGVNGS
                     GKTTLLRTLTGERPLQGGRLVKGTTVQLAELSQELVDLPKDMRVLEATEQVAKYVRLG
                     KLELTASSVLERLGFPAARQWTPVGQLSGGERRRLQLTRLLMAEPNVLLLDEPTNDLD
                     VDTLARLEDLLDGWPGTLVVVSHDRYLVERACDTVVALLGDGKITHLPGGIEEYLQRR
                     AAAGTQATGLSAAPAGPAVAKPASSAADQRAARKEASRLERRMTALSKREKQLHEELA
                     AAATDPARLLELDAELKAVLAEQESVELDWLAAAELAES"
     gene            complement(1922..2881)
                     /locus_tag="VFE39_12590"
     CDS             complement(1922..2881)
                     /locus_tag="VFE39_12590"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HET6259481.1"
                     /translation="MLAAVPPPVTVRVPAKINLHLAVGPLREDGYHDLVTVFHAVSLF
                     DEVSLAPSDAPGIEVHGEGVAEVPADETNLAWRAVQLLAERADRDPDVRLVLRKGIPV
                     AGGMAGGSADAAAALVGLSALWKLDLSRDELAVLAAELGSDVTFALYGGTALGTGRGE
                     RIVPVLSRHPQHWVIALHRGGLSTPSVFRELDRLRGGGKPPDRPVEPVLEALTGGDPR
                     QLALSLGNDLQAAAVSMAPELRRTLRAGVGAGALAGLVSGSGPTCAFLCSNADAAIDV
                     ATELAGVGVCRTVRVAHGPVPGARVIDQYEPPGPPTSWPPVRA"
     gene            complement(2943..4472)
                     /locus_tag="VFE39_12595"
     CDS             complement(2943..4472)
                     /locus_tag="VFE39_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HET6259482.1"
                     /translation="MATRRLTDTAAEAAPSSPWPALWALVVGFFMILVDSTIVSVATP
                     AIMGGLGAGVDSVVWVTSAYLLAYAVPLLITGRLGDRFGPRRVYLAGLAVFTLASLWC
                     GLTGSIGELVAARVVQGLGASLMTPQTMAVITRTFPAESRGKAMSLWGATAGVATLVG
                     PILGGVLVDSLGWEWIFIVNVPIGVVGLVLAARLVPELPTHVRRFDLLGVALSAIGMF
                     LLVFGIQEGQTYDWGTITGPISVWSLIVAGLVVMAGFLFWQARTRGEPLVSLGLFRDR
                     NFSLANVAITTVGFAVTAMTFPIIFYAQGVLGMTPTGSALLLVPMAVVSGGLAPIVGR
                     WTDRVHPSWIAGIGMVCFPAALLWLAAIIGPTTPVWQLLLPIALLGVANGFVWAPIAT
                     TATRNLPPSNAGGGAGIYNTTRQIGAVLGSAGIAALIESRLAANLPASPAGGSSEVGV
                     SQLPEALHAGFATAMGQALVLPAVVLLIGLVAVLLFARPRHLSHPLPAETAAPVLPAA
                     K"
     gene            complement(4454..5020)
                     /locus_tag="VFE39_12600"
     CDS             complement(4454..5020)
                     /locus_tag="VFE39_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET6259483.1"
                     /translation="MAAALTSLATSILAMVCERPMHPYEMYRLMMDRFEDRVVKVRPG
                     SLYHTVARLAADGLVEAIGTDREGGRPERTTYRVTDAGRAAMQEWIRGVLAKPVNEFP
                     RFPVALGEAHNLSRAEAIELLTARIRHIEAELAEVEPALAHGATKTAEARLLDGYYLV
                     EMLRAELSWLRRLVARLETKELGWPLDD"
     gene            complement(5099..5953)
                     /gene="rsmA"
                     /locus_tag="VFE39_12605"
     CDS             complement(5099..5953)
                     /gene="rsmA"
                     /locus_tag="VFE39_12605"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010316339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET6259484.1"
                     /translation="MTDEQSRLLGPAEVRALAGELGLRPTKRLGQNFVHDPNTVRRIV
                     RAAELAPDDVVLEVGPGLGSLTLALLPAVRAVHAVEIDPVLAARLPGTAAERAPALAG
                     RLTVTAADALRLRAEDLSGPVPTVIVANLPYNVGVPVVLHLLAELPQLRRGLVMVQSE
                     VAERLAAPPGSKAYGVPSAKLAWFAAARRAGPVPRAVFWPVPNVDSGLLAFDRRDPPP
                     GNRVEVFTLIDAAFAQRRKALRSALSGWAGSPAAAEEALRAAGISPLERAEQLDVTAF
                     ARLAAARP"
     gene            complement(6018..>7465)
                     /locus_tag="VFE39_12610"
     CDS             complement(6018..>7465)
                     /locus_tag="VFE39_12610"
                     /inference="COORDINATES: protein motif:HMM:NF018447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HET6259485.1"
                     /translation="MTRPAAGATAIDAGADPTTGPFGRVPAQELTGRILPFDDEMTGP
                     IAVVAAVAAAPTVEAEPLTQPFAAVTAPEAAPETDAIAVVEAPVSSAVLDAPTRAARR
                     RRASAPRRSPARALVRGTVLAVLIALVGGSATALAMDKTITITVDGQDRTLHTFATDV
                     ASALESAGLSAAPQDRVEPALPTELGDGDHIILSRARKLTLVEGDSERQVWTTAASVG
                     EALQGLGVAATPIQMSAAPTTSIPLAGLALKLNVPRAVSITDGTGAPAQVTTTAGTVA
                     GLLAERGIELGPDDVSVPPGDTPLTDGVSVQVVRNGVGEVVVTRPIPPPEQEIEDPEM
                     ARGKRTVVDRGKPGEQTAIMRVYVQNGKEVRREQVRAGSSTPPEPRVVKVGTNDNIPQ
                     APAISDGSVWDRLANCEATGNWAINTGNGYYGGLQFDAGTWRAYGGTDYAPLPHEASR
                     EEQIDVASKVRDDRGGYGAWPACASKLGLPR"
BASE COUNT         1061 a         2771 c         2667 g          966 t
ORIGIN      
        1 caccgcacgt cacgccgcag cgcgcccacg ggcgttgcgc gagcaccggt cgagggtcgt
       61 gcgcggatat cggtgctacg gcactcgttt ccgcgcacgg gcagctacga ctcggcgagc
      121 tccgcggcgg ccagccagtc cagctcgacg ctctcctgct cggcgagcac cgccttcagc
      181 tccgcatcga gctccagcag ccgggcgggg tcggtggcgg ccgcggcgag ctcctcgtgc
      241 agctgcttct cgcgcttgct cagcgccgtc atccgacgct ccagcctgga ggcctccttg
      301 cgggcggcgc gctggtcggc ggcgctcgac gcgggcttcg ccactgccgg gcccgcaggc
      361 gcggcagaga ggcccgtggc ctgcgtgcct gccgccgccc gccgctgcag gtactcctcg
      421 atcccgccgg gcaggtgcgt gatcttcccg tcgccgagca gcgcgaccac cgtgtcgcag
      481 gcccgctcca ccaggtaacg gtcgtggctg acgacgacga gcgtgcccgg ccagccgtcg
      541 agcaggtcct ccagccgcgc cagagtgtcg acgtcgaggt cgttggtggg ctcgtcgagg
      601 agcaggacgt tgggctcggc catgagcagc cgggtcagct gcagccgtcg ccgctcccca
      661 ccggacagct gcccgacggg cgtccactgg cgggctgcgg gaaagccgag ccgctccagc
      721 accgacgacg ccgtcagttc gagcttgccg agccggacgt acttcgccac ctgctcggtg
      781 gcctccagca cacgcatgtc cttcggcagg tcgacgagct cctggctgag ctcggccagc
      841 tgcacagtgg tgcccttgac cagccggccg ccctgcagcg ggcgctcgcc ggtgagcgtg
      901 cgcagcagcg tggtcttgcc ggagccgttg acgccgacga tgccgacgcg gtcgcccggg
      961 ccgagccgcc aggtgacgtg gttgagcagt gtgcggccgg cgatctcagc agtagcgtcc
     1021 tccaactcca gtaccgtcct gccgaggcgg ttggtggcga acccgagcag ctccacggtg
     1081 ttgcgaggcg gcgggacgtc ggcgatcagc gcctcggcgg cctcgatccg gaaccgcggc
     1141 ttcgacgtgc gcgccggcgc tccgcgacgc agccaggcca gctccttgcg ggcgaggttc
     1201 tgcctgcggg cctccgcggc gtcggcctgc cgcgagcgct cggcgcgggc gtagacccag
     1261 tcggcgtagc caccgaggta gctctccacc cggccgttcg cgacctccca cgtgcgggtg
     1321 cagaccgcgt ccaggaacca acggtcgtgc gtgacgacca cgaccccgca tcgcctcgac
     1381 aacaggtgct cggcgagcca cccgatgccc tcgacgtcga ggtggttggt gggctcgtcg
     1441 aggacgacga ggtcgggatc gccgacgagc gccgcggcca gcgccacccg tcgcttctct
     1501 ccgccggaca ggccctcgac gctccgttcg aggtcgccga tgccgagccc gtcgagcacg
     1561 tcgcggacct tggcgtcggc ggcccactcg tggtcggcgg ccccgtgcca ggccgcgagc
     1621 acgacgtcgc ggacgcgggc accaggcggg agcgtgtcgc cctgcgcgag gtgcgccagg
     1681 ttcaggtcgc cgacgcggct gacccgcccg ccgtccgggg cgcggtcgcc cgtgatgatg
     1741 tcgagcagcg ttgtcttgcc accaccgttg agcccgacca cgccgacccg ctcgctgcgc
     1801 tcgatgccca gcgacacggc gtcgagcagt acgcgcgagg cgtcgccggg gacgtgtgcg
     1861 gtgaccgact ccaggttcac cagattctgg gccgatctct gggttgctcc gcgtggggcc
     1921 atcaggcacg caccgggggc catgaggtgg gcgggccggg cggttcgtac tggtcgatca
     1981 cccgggcacc ggggaccggg ccgtgcgcca cgcgaacggt gcggcacacg cccaccccgg
     2041 ccagctccgt cgccacgtcg atcgcggcgt cggcgttcga gcacaggaag gcgcacgtcg
     2101 gcccggaacc ggagacgagc ccggcgagcg cacccgcgcc aaccccggcg cgcagcgtgc
     2161 gccgcagctc aggagccatg ctgacggcgg cagcctgcag atcgttgccc aggctgagtg
     2221 cgagctgacg aggatcaccg ccggtgagtg cctccagcac cggctcgacc ggacggtcgg
     2281 gcggcttccc gcccccgcgc agccggtcga gttcgcggaa gacgctcggc gtggagagac
     2341 caccacggtg cagcgcgatc acccagtgct gcgggtgccg ggagagcacc gggacgatcc
     2401 gctcgccgcg cccggtgccg agcgcggtac cgccgtagag cgcgaacgtg acgtcgctgc
     2461 ccagctcggc ggccagcacg gccagctcgt cgcgggaaag gtcgagcttc cacaacgccg
     2521 acagcccgac gagtgcggct gccgcatcag cgctcccgcc cgccatcccg ccggcgaccg
     2581 ggatgccctt gcgcagcacc agtcgcacgt ccgggtcgcg gtcggcgcgc tcggccagga
     2641 gctggaccgc gcgccatgcg aggttcgtct cgtcggcggg cacctcggcg acaccctccc
     2701 cgtgcacctc gatccccgga gcgtcactgg gtgcgagaga cacctcgtcg aacaggctca
     2761 cagcgtggaa caccgtgacg aggtcgtggt agccgtcctc gcgcagcggc ccgacggcaa
     2821 gatgcaggtt gatcttcgcg ggaacgcgca cggtgaccgg gggagggacg gcagcgagca
     2881 cccgacaaca ctacgtgcgt ctactcccgg cccgagcggc gcgggccacg aatcggcgtc
     2941 cttcacttgg cggccggcag gaccggcgcc gccgtctcgg cgggcagcgg gtggctcagg
     3001 tgccgcggcc gcgcgaacag cagcaccgcc accagcccga tcagcagcac cactgcaggc
     3061 aacaccagcg cctgccccat cgccgtggcg aaacctgcgt gcagcgcctc cggaagctgt
     3121 gacacaccga cctccgaact cccgcctgcc ggagaggccg gcaggttcgc cgcgagccgc
     3181 gactcgatca gtgcggcgat ccccgcactg cccagcaccg caccgatctg gcgggtcgtg
     3241 ttgtagatcc cggcgccgcc accggcgttc gagggaggca ggttccgcgt cgccgtggtg
     3301 gcgatcggag cccacacgaa cccgttggcc acgcccaaca gggcgatcgg aagcaacagc
     3361 tgccagaccg gggtggtcgg cccgatgatc gccgcgagcc acagcaacgc cgccgggaag
     3421 cacaccatcc cgatgcctgc gatccagctc gggtgcacac ggtcggtcca ccggcccacg
     3481 atcggcgcga gaccgccgga cacgacggcc atcggcacca gcagcagcgc agagccggtc
     3541 ggggtcatcc cgagcacgcc ctgggcgtag aagatgatcg ggaacgtcat cgcggtgacc
     3601 gcgaacccga ccgtggtgat cgcgacgttg gcgaggctga agttccggtc gcggaacagc
     3661 cccagcgaca ccagcggctc tccgcgggtg cgcgcctgcc agaacaggaa gcctgccatc
     3721 accaccaggc cggcgacgat cagcgaccac acggagatcg ggccggtgat cgtcccccag
     3781 tcgtaggtct gcccctcctg gatgccgaag accaacagga acatgccgat cgcgctcagc
     3841 gccacaccga gcaggtcgaa gcggcggacg tgggtcggga gctcgggaac cagccgggcc
     3901 gccagcacga ggccgacgac gccgatcggc acgttgacga tgaagatcca ttcccagccg
     3961 aggctgtcca cgagcactcc gcccaggatc ggcccgacga gcgtggccac cccggcagtg
     4021 gcaccccaca ggctcatcgc cttgccccgg ctctcggcgg ggaaggtccg cgtgatcacg
     4081 gccatcgtct gcggggtcat cagggacgcg ccgaggccct gcacgacccg cgcggcgacg
     4141 agctcgccga tgctgccggt caggccgcac cacagggacg ccagcgtgaa gacggccagc
     4201 ccggcgaggt agacgcggcg cggtccgaac cggtcaccca gcctgccggt gatcagcagc
     4261 ggcaccgcgt aggcgagcag gtaggcgctc gtgacccaca cgaccgagtc cacgccggca
     4321 ccgaggcctc ccatgatcgc gggagtggcg acggagacga tcgtcgagtc caccaggatc
     4381 atgaagaacc cgaccaccag cgcccacagc gcgggccacg ggctggacgg cgcggcctca
     4441 gcagccgtat cggtcagtcg tcgagtggcc atcccagctc cttggtctcc agccgagcca
     4501 cgagccgccg cagccagctc agctcggccc gcaacatctc cacgaggtag tacccgtcca
     4561 gcaggcgggc ctcagcggtc ttggtcgccc cgtgtgcgag ggccggctcc acctcggcga
     4621 gctccgcctc gatgtggcgg atgcgtgccg tgagcagctc gatcgcctcc gcgcgcgaca
     4681 agttgtgcgc ctcgccgagg gcgaccggga accgggggaa ctcgttgacc ggcttggcga
     4741 gcacgccgcg gatccactcc tgcatcgcgg ctcgcccggc gtcggtgacg cggtacgtgg
     4801 tgcgctcggg acgcccgccc tcgcggtcgg tgccgatcgc ctcgaccaga ccgtcggccg
     4861 ccagccgggc gacggtgtgg taaagcgagc ccgggcgcac cttgacgacc cggtcctcga
     4921 accgatccat catcagccgg tacatctcgt agggatgcat cggccgctcg cacaccatcg
     4981 ccaggatgct ggtcgccagc gacgtcagtg ccgccgccac acgcaccact ccgctcggat
     5041 cgttccattg gggttattcc gcgcggaata gtacacgtgg aacccccgcg ggcggcggct
     5101 acgggcgggc ggcggccagg cgagcgaacg cggtgacgtc gagctgctcg gcgcgctcca
     5161 gcggcgagat tccggctgcg cgcagagcct cctccgccgc agccggcgag cccgcccagc
     5221 ccgacagcgc cgagcgcagg gccttgcgac gctgcgcgaa cgccgcgtcg atcagcgtga
     5281 acacctcaac gcgattgccg ggtggcgggt cgcggcggtc gaacgccagc agcccggagt
     5341 cgacgttcgg cacgggccag aacaccgctc ggggcacggg acccgctcgg cgggccgcgg
     5401 cgaaccaggc cagcttcgcg ctcggcaccc cgtatgcctt gcttccgggc ggggcggcga
     5461 gccgctctgc aacctcggac tggaccatca cgagcccgcg ccgcagctgc ggcagctcgg
     5521 caaggagatg aagcaccacg ggcacgccga cgttgtacgg caggttcgcg acgatcacgg
     5581 tgggcaccgg accgctcagg tcctcggctc gcaggcgcag cgcgtcggcc gccgtgacgg
     5641 tgagccggcc ggccagcgcc ggggcccgct cggccgcggt gccgggcagc cgcgcggcaa
     5701 gcacggggtc gatctccacg gcgtgcaccg cgcgcacggc cggcagcagg gccagcgtca
     5761 gcgacccgag ccccggtccc acctcgagca cgacgtcgtc gggtgcgagc tccgctgccc
     5821 ggacgatacg acgcacggtg ttcgggtcat ggacgaagtt ctgtccgagc cgcttggtgg
     5881 gccgcagccc cagctccccc gccagtgccc gtacctccgc gggccccagc aaccgcgact
     5941 gctcgtccgt caccccggga gggtgcaccg gtctgctcgg ccggccgtca ccggggccgt
     6001 cagcagccgg tcaggcatca ccggggcagg ccgagcttgc tcgcgcaggc cggccaggcg
     6061 ccgtagccgc cgcggtcgtc gcgaaccttg ctggccacgt cgatctgctc ctcgcggctg
     6121 gcctcgtggg gcagcggggc gtagtcggtg ccgccgtagg cgcgccaggt accggcgtcg
     6181 aactgcaggc cgccgtagta gccgttgccc gtgttgatcg cccagttgcc ggtggcctcg
     6241 cagttggcga gccggtccca cacactgccg tcggagatcg ccggggcctg cgggatgttg
     6301 tcgttcgtgc cgaccttgac cacccgcggc tccggcggtg tgctgctgcc ggcccgcacc
     6361 tgctcgcgcc ggacctcctt gccgttctgc acatacacgc gcatgatcgc cgtctgctcg
     6421 ccgggtttgc ccctgtcgac gaccgtgcgc ttgccccggg ccatctcggg gtcctcgatc
     6481 tcctgctccg gcgggggaat cggccgggtc acgacgacct cgccgacacc gttgcgcacc
     6541 acctgcaccg acacgccgtc ggtgagcgga gtgtcaccgg gcggcacgga gacgtcgtcc
     6601 ggcccgagct cgatgccccg ctccgcgagc aacccggcca cggttcccgc cgtcgtggtg
     6661 acctgcgccg gcgctccggt gccgtcggtg atcgagacgg cacgcggcac gttgagcttg
     6721 agcgccaggc cggcgagcgg gatgctcgtg gtgggcgcgg cggacatctg gatcggggtg
     6781 gccgccacac cgagcccctg cagggcctcg ccgaccgacg ccgcggtggt ccagacctgt
     6841 cgctccgagt caccctcgac gagggtgagc ttgcgggcgc ggctgaggat gatgtggtcg
     6901 ccgtcgccca gctcggtggg cagcgcgggc tcgaccctgt cctgcggggc cgcggagagc
     6961 ccggccgact ccagcgcgga tgcgacgtcg gtggcgaacg tgtgcagggt gcggtcctgg
     7021 ccgtccacgg tgatcgtgat ggtcttgtcc atggccagcg ccgtggcact gccacccacc
     7081 agtgcgatga gcacggcgag gacggtgccg cgaacgagcg cgcgcgcggg ggaccgccga
     7141 ggcgcggagg cccgacgccg tcgcgcggcg cgagtgggcg cgtcgagcac tgccgacgac
     7201 acgggcgcct cgacgaccgc aatcgcgtcg gtctccgggg cggcctccgg cgccgtcacc
     7261 gccgcgaacg gctgcgtcag gggctctgcc tcgaccgtag gcgcggcggc caccgccgcg
     7321 accaccgcga tcgggcctgt catctcgtcg tcgaacggca ggatccgacc ggtgagctcc
     7381 tgcgccggga cccggccgaa cggacccgtg gtcggatcgg cgcctgcgtc gatggcggtg
     7441 gcgcctgccg ccggccgcgt catca
//
LOCUS       DASRSC010000258        16130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1082991,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000258 DASRSC010000000
VERSION     DASRSC010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 16130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16130
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082991"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1474
                     /locus_tag="VFE39_12615"
     CDS             <1..1474
                     /locus_tag="VFE39_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6259486.1"
                     /translation="VRRRLAFEDDPAGFAATWSTAEAGTAARIAAAQARVGSVVLPDV
                     ELRRIAAVCAAFDVDGMRADLVIARAAVAHAAWRGSDMVAEEDVRVAARLALPHRRRR
                     DPFDEPGMDEDALDAALQEAAQQEPTQEEATQQPPDGPGDGEHGGTPDDGPPDDGGPD
                     DPGSGGGNPPDGGGAPPPQDGTPAPEPSRSDADQQPTGAGAGGHRPGGDGRSVAAPPP
                     SRPFRARLLQVPGIGEGTPGRRSRARTDVGQVVRASGQSPRRAADLHLPATIAAAAPY
                     QLARGRAGAGLLLRTGDLRRAEREGREGNLVLFAVDASGSMAARARMSAVSGAVLSLL
                     RDAYQRRDKVGLVTFRGAGAELVLPPTSSVPAAQVRLAAMRTGGRTPLADGLLRARAV
                     LRVERLRDPRRRPLLVLLTDGRATVAARVGGDPVHDACRAAALLASDGVATIVVDCES
                     GPVRLGLAARIAAAADAPTVGVAELSADHVAGVVRAARAA"
     gene            1502..2116
                     /gene="cobO"
                     /locus_tag="VFE39_12620"
     CDS             1502..2116
                     /gene="cobO"
                     /locus_tag="VFE39_12620"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HET6259487.1"
                     /translation="MPQGQVTTVPEDGLTTRQRRNRPLVVVHTGEMKGKSTAAFGLAL
                     RGWSQGWSIGVFQFVKSAKWKVGEEAAFRALGRVHTETGEGGSVEWHKMGEGWSWTRK
                     QGTEDDHAAAAAEGWAEIRRRIEAQAHDVYVLDEFTYPMKWGWVDVAEVVEVLAARQG
                     RQHVIITGRDAAPELVAAADLVMQTTKIKHPMDSGQKGQRGIEW"
     gene            2151..3125
                     /locus_tag="VFE39_12625"
     CDS             2151..3125
                     /locus_tag="VFE39_12625"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET6259488.1"
                     /translation="MEYTRLGSTGLQVSRVCLGMMSYGSSSWRDWVLDLDASRPLVRS
                     AVDAGITFFDTADVYSLGVSEEVTGTLLGEFFSRREDYVLATKVFSTMSDRPNDRGLS
                     RKHIMSSIDASLRRLGTDHVDLYQIHRFDSETPIEETMEALHDVVKAGKARYIGASSM
                     YAWQFAKAQYTADLGGWTRFVSMQDHYNLIYREEEREMHPLCLDQGVGVIPWSPLARG
                     RLARVAEQRSTTTRSGSDPIGDRMYVEADDAVVHAVADVAKARDLPLAQVALAWMLHQ
                     EAVTAPIVGATKPGHVEDAVAAVDVELSDDEIAALEAPYVPHPVLGHQ"
     gene            complement(3170..3436)
                     /locus_tag="VFE39_12630"
     CDS             complement(3170..3436)
                     /locus_tag="VFE39_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259489.1"
                     /translation="MKAFAIWFAVGFVGAGTYFGSAIVGMVAGVILYLVTVQVWPWTL
                     CHACEGIPRRHRDWGEAAHWRTCGACKGTGKEPRALAHPSRVQW"
     gene            3768..5159
                     /locus_tag="VFE39_12635"
     CDS             3768..5159
                     /locus_tag="VFE39_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HET6259490.1"
                     /translation="MTARAVVIGAPSSGSGKTTVATGLMAALHRRGTTVAPFKVGPDY
                     IDPGYHTLATGRPGRNLDPVLVGAERIAPLARHGAAGAEVAVVEGVMGLFDGRLADGH
                     GSTAQVAGLLGAPVVLVVDCRGQSRSLAALLHGFRSFDPSVRLAGVVLNRVGSARHEA
                     VLRAAADEAGLPVLGALPRHDALAVPSRHLGLVTAAEHGPAAVAAVDAMAELAATHVD
                     LDAVLRLAEPLPPGPAWSAADAARARIRVPEHSYPRTAVFGGPAFTFGYAEHVELLAA
                     AGAEVVVVDPLRDEALPDGTAALVLPGGFPEEHVAALGANEPLRDAVAALAASGAPVH
                     AECGGLLYLCESLDDAPMCGVLPATAAMTGRLTLGYRDAVALEGSSLFPAGARVAGHE
                     FHRCAVAPRAGVPTVGAAPAWGWRSGEPEGLVTEGWVAGGVHASFLHTHPAGDPEAVS
                     RFVAAAAETPGRR"
     gene            complement(5181..6218)
                     /locus_tag="VFE39_12640"
     CDS             complement(5181..6218)
                     /locus_tag="VFE39_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HET6259491.1"
                     /translation="MKAIIYDQPGDASVLRLVDREIPEPGAGDVRIKVIVSGVNPTDW
                     KSRSGALGGEPDPIERVPNQDGAGIVDAVGPGVDGVAVGDRVWVVLAAHERPTSGTAQ
                     EYTVVPAERVFPLPAGADFDLGASIGVPAVTAHRALTVAEGGPARLRPGTLDGRVVLV
                     AGGAGAVGHAAIQLARWAGATVITTVSSPTKAAYATAAGAHHVVNYTDADAVSQIRGI
                     APDGVDLVVEVAIGRNAELDLQVLRPRGTISIYANDGGAPFTVDVRRNMGLNTRYQFV
                     LLYTVGRENITAAAEDINAAIADGALPVGEAAGLPLHHFTLEQAAEAHRAVESGVAGK
                     VLIRVAAEPAS"
     gene            6521..7357
                     /locus_tag="VFE39_12645"
     CDS             6521..7357
                     /locus_tag="VFE39_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005165184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6259492.1"
                     /translation="MELAFERVGPADGEPLLLIMGLGMQMLFWHDDLCAEFTRAGFSP
                     ARFDNRDVGASTHLTHLGAPPLPVVIALPQAVAAYRVSDMAADAVAVLDALGWESAHI
                     VGVSMGGMIAQTVAIEHPHRVRSLTSVMSTPSPRIGRPTLGAAAALAARPGRTAEEAA
                     QRVVDTFRVIGSPGYPLDEDWLRNYARRAFARSKDSTGTRRQLAAVNASPSRVAGLRS
                     VRAPTLVVHGEADPLVGPEAGRATAAAVPGARLVTYPGMGHNLPRELWPSIVRQVAGL
                     VG"
     gene            complement(7370..8839)
                     /locus_tag="VFE39_12650"
     CDS             complement(7370..8839)
                     /locus_tag="VFE39_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HET6259493.1"
                     /translation="MTGVVLGVDIGTSSSKAVLVTLDGAILASAVREHEVSCPQPGWF
                     EMDAGLWWSEFVLLARELLAGRDVDVTAVGVSGMGPCVAVTDAVGTPLRPAILYGVDM
                     RATAQIRRLEDELGAQDILDRGGSALSTQAVGPKLLWIAENEPETFARVRRLFMPSSW
                     LVHQLTGAYVLDHHSASQCSPMYDTEAHAWYEPWVRAIAPDLELPELRWPGEAAGTVT
                     AEAAAVTGLRVGTPVITGTIDAWSEAVSVGAHRVGDLMLMYGTTLFLINTVAQRRTSP
                     VLWSTVGALPGTRNLAGGMATSGAITAWLRRLAGSPDYTTLLDAAGRSGPGARGLLML
                     PYFAGERTPVADPEARGVIVGLTLEHTAGDLYRAALEATALAVRHNVEFFREAGGQID
                     RVVAVGGGVRGALWTQIVSDVTGLPQQIPSVTIGASFGAAFLAGGLVAAPSINVWNPV
                     RTEVTPQPALAATYHRLYGLYRELYASTASVVHQLGRGG"
     gene            complement(8836..10485)
                     /locus_tag="VFE39_12655"
     CDS             complement(8836..10485)
                     /locus_tag="VFE39_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
                     /protein_id="HET6259494.1"
                     /translation="MTTYALPEVREHGTTPPATAFLIASGDLRPSANVAGWPTQAGVE
                     ESLTRAFADLGWTLRRANPVDPEKGHGFIDSQRMGLTVFASIPPDAPLVVAEAVWQYS
                     HHVLAGLRTHRGPILTVANFVGDWPGLVGLLGLNAGLTKMGTPYSTLWSVDFTDSWFR
                     DGLRSWVETGRIEHDANHVRPLPALPAIPEKELGEALADQLQTEKAIIGVFDEGCMGM
                     YNAIIDDELLNPLGIFKERLSQSALYAEMLRVPDEDAAAVGSWLAERGMRFLTGTDDA
                     TELTEAQLSWQYKMYIAALRISDDFGLDAVGIQYQQGLKDLVPASDLAEGLLNNVERP
                     PVTSRDGSRVLWEGLALPHFNEADEGVAVDALLTNRVWSAMGLDPATTLHDVRWGEQF
                     GDEFVWVFEISGSVPPSHLRNGYADAVGWRQSPVFFPAGGSTIRGVSKPGEIVWSRVY
                     IAEGGLHLDIGRATAVELPEEETQRRWAATNPEWPIMHVVLHGITRNQFMARHKANHA
                     QVAYAPDAETADRALLAKAALFARLGVTVHLCGDVPLPETP"
     gene            10771..11769
                     /locus_tag="VFE39_12660"
     CDS             10771..11769
                     /locus_tag="VFE39_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6259495.1"
                     /translation="MVKINDVAREAGVSAATVSRTLNGRSSVDPVLAARVREAASRLG
                     YRPNGVARSLRRQVTDVLALIITDVSNPFFTAITRGVEDVAQSRGLSVLLCNSDEDPG
                     KESTYLHVAEQEQVAGVILSPHRTGSDVAHLRRASIPMVVIDRPLDEPVDSVMVRSRE
                     GARAATNHLIDAGWTRPACITGPEDAWTAQERLEGYLEAVREHGELAELWVHAPFRQQ
                     GGMTGAARLLDAPEPPDALFVANAQMALGVLEEVRRRGLRVGVDLGVITFDDAPWAPF
                     ISPPMSVVAQPAYDVGAQAAQLLMERVKGTAPDAPRHLWLSTMLIVRESSRRHGAG"
     gene            11970..12872
                     /locus_tag="VFE39_12665"
     CDS             11970..12872
                     /locus_tag="VFE39_12665"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HET6259496.1"
                     /translation="MLEYGHCHAQPLGCIFYGEWSGRTREFAYSYVYVEGAGHKILVD
                     VGHDIRSTNKAWNDDNDIVDYQAPDAVLAKVGVRPEDIDTVVLTHAHYDHAGAARWFP
                     NARFYLQRRELESSRWALGQSPLYDSIIAALDPADVTMLEEMARDGRLVLLDGALQLF
                     PGLEIRTAWDTHTQGGQYVVITDHDDHRWVVTGDAMYSYENAEGIDGSGRYVNIGFGG
                     GSGWDGLRLIDEMVMTAGSTDRLVIVHESATFARNPSRRYDDRLSVAELLLAPGQPSR
                     IGAPPAGCDPDLSPAAVLPSPEDS"
     gene            12884..13864
                     /locus_tag="VFE39_12670"
     CDS             12884..13864
                     /locus_tag="VFE39_12670"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET6259497.1"
                     /translation="MPRNRAVLAMAGLLVLGLAACSVPSGNGAAPTGGTSTSKVVGIV
                     TISATDANNARVIAAVTESAKAAGWTVDVVDAAGNADQANSAMRNFVSKGDGMIFDLV
                     FPTSSLGAGLASARQAGVPVATWGGGLGTGVVMTTGDGAPFAQPATQKMVDDLGGSGS
                     VLALTYRAGQVCRDREGVFDQIMSTNPGVQVSKEEVTIPGFLQDGSKYATAWLAGHPQ
                     GSGPLAIWGCWEDPTLGAISALKQQNRTDVKTYGINGSSTAIKAVQDGSLTATVYEDG
                     AAEGKTMFDTTMQAIQAGAGWQPKTVNVPGILVTAQTVGQFVQDHPEALK"
     gene            13909..15417
                     /locus_tag="VFE39_12675"
     CDS             13909..15417
                     /locus_tag="VFE39_12675"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6259498.1"
                     /translation="MSKAFGGVQALAGVSFEIRPGVVHGLVGANGAGKSTLIRCLAGI
                     GLPDSGSIEVDGMPVSISSTDDATRLGLAFIHQEMSLIPGWDVLRNMALGVRPRTRFG
                     VIDWRSTRQRAAEVARRLGMRFGLGSPIGDLSTADQWLVLIGRALMRDARMIAMDEPT
                     ASLSVEEATRLHGIVRELAAAGTAIIFVSHRLDEVSALCEDVTVFKDGRVTKRVVGEP
                     TSKSDLVRAIVGRDLEIPEHGQEAVRAGRTVLEVRDLSDGARVQDVSLTVHEGEVVGL
                     GGLVGAGRTELVKMIYGAERSAGGEVLLDGQRVSFRSPADAVRAGMGLVPEERRSEGL
                     FLDRTIDFNINVATLGSLRRSRWLPLLRSAAGRRRAQSVADLVAVKAESVNVPVGSLS
                     GGNQQKVAIARWLLETPRLLILDEPSRGVDVGARAEVHRVVRELASRGAAVLAVSSDN
                     EELVALCDRVLVMAEGRVTGELSGTEITADRIVSLSFARGSGVGAAAEGATA"
     gene            15414..>16130
                     /locus_tag="VFE39_12680"
     CDS             15414..>16130
                     /locus_tag="VFE39_12680"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6259499.1"
                     /translation="MSTETAGPVDVAPPPGAPRPHRPASRIGLMIVSKYGTLIAMGIM
                     IVIFGTSVPNGAFLTSNNLLNILNQSALTAIIAFGLTMVLVVGEFDLSIGYAASLAGV
                     LVTGLLVNQGLALPVAILLTLLLGAAIGAVNGLLVTKARVNAVVATLGVGTMLIGLSY
                     GYTAGSPIIAVPTSFTNITLGAFIGVQNPIWFMVIVLGLLWVLLNRTPLGQRIQAVGA
                     NTEAARLAGIRTDRVKVAAFV"
BASE COUNT         2270 a         5695 c         5831 g         2334 t
ORIGIN      
        1 ggtgcggcgc cggctcgcct tcgaggacga ccccgcgggc tttgccgcca cgtggagcac
       61 cgcggaagcc ggcaccgcgg cgcgcatcgc cgcggcacag gcccgggtcg gctccgtggt
      121 gctgccggac gtcgagctgc gccggatcgc cgccgtctgc gcggcgttcg acgtcgacgg
      181 catgcgtgcc gacctcgtga tcgcccgcgc cgccgtggcg cacgctgcct ggcgtgggtc
      241 cgacatggtg gcggaggagg atgtacgggt ggccgcgcgg ctcgcgctgc cgcaccggcg
      301 ccgccgtgac ccgttcgacg agccggggat ggacgaggac gccctcgacg cggccctgca
      361 ggaagcggcc caacaggaac cgacccaaga ggaagcgacc cagcagcccc cggacggccc
      421 cggggacggt gagcacggcg gtactcctga cgacgggccc cccgacgacg gtggtcccga
      481 cgacccgggg tccggcgggg gaaatccacc cgacggaggc ggcgcacccc cgccgcagga
      541 cgggacgccc gcgcccgagc cctcccggtc cgacgctgat cagcagccca ccggcgcagg
      601 agccggcggt cacagacctg gcggcgacgg gcggagcgtg gccgctccgc caccgtccag
      661 gccgttccgg gcgcgcctgc tgcaggtccc cgggatcggc gagggcaccc ccggccgccg
      721 gtcgcgggcg cgcaccgacg tcggccaggt cgtccgcgcg tccggacagt cgccgcgtcg
      781 cgccgccgac ctccacctgc ccgccacgat cgccgccgcc gccccgtacc agctggcccg
      841 cggccgggcg ggcgcgggcc tgctgctgcg caccggtgac ctgcgccgcg cggagcgtga
      901 gggccgcgag ggcaacctcg tcctgttcgc cgtggacgcc tcgggctcga tggccgcgcg
      961 ggcgcggatg tccgccgtca gcggggcggt cctctcgctg cttcgggacg cctaccagcg
     1021 ccgtgacaag gtcgggctcg tcacgttccg gggcgcaggg gcggagctcg tcctgccgcc
     1081 cacgtccagc gttccggcgg cgcaggtccg gctcgcggcg atgcgcaccg gcggtcggac
     1141 cccgctcgct gacggactgc tgcgggctcg ggcggtgctg cgcgtcgaac ggttgcgtga
     1201 tccgcgccgt cgtcccctgc tcgtgctgct caccgacgga cgggccacgg tcgcggcgcg
     1261 ggtgggtggc gaccccgtgc acgacgcgtg ccgagccgcg gccctgctcg cgtccgacgg
     1321 cgtcgcgacg atcgtcgtgg actgcgagag cggcccggtc cggctcgggc tcgcggcccg
     1381 catcgccgcc gcggcggacg ccccgacggt gggcgtcgcc gagctgtcgg ccgatcacgt
     1441 ggcaggtgtc gtccgcgccg cgcgagcggc gtagctcgcg acccccgacg aaggaggaac
     1501 aatgccgcag ggccaggtca ccaccgtgcc ggaggatggg ctcaccaccc gccagcgccg
     1561 gaaccggccg ctcgtcgtcg tccacacggg ggagatgaag ggcaagtcga ccgccgcatt
     1621 cgggctcgcg ctgcgcggct ggagccaggg ctggtcgatc ggggtgttcc agttcgtcaa
     1681 gtcggcgaag tggaaggtgg gcgaggaggc ggcgttccgt gcgctcggcc gcgtgcacac
     1741 cgagaccggt gagggcgggt ccgtggagtg gcacaagatg ggcgagggct ggtcctggac
     1801 ccgcaagcag ggcaccgagg acgaccacgc cgctgccgcc gccgagggtt gggcggagat
     1861 ccgccgccgc atcgaggcgc aggcgcacga cgtctacgtg ctcgacgagt tcacctatcc
     1921 catgaagtgg ggctgggtgg acgtcgccga ggtggtcgag gtgctggctg cccggcaggg
     1981 ccgccagcac gtgatcatca ccggccgcga cgccgcgccg gagctggtgg cggcggccga
     2041 tctcgtgatg cagaccacga agatcaagca ccccatggat tccggacaga aggggcagcg
     2101 cggcatcgag tggtgatccg ctgcccgggt cggggctaag gtcgtcagac gtggagtaca
     2161 cacgacttgg ctcgaccgga ctgcaggtct cgagggtgtg cctggggatg atgagctacg
     2221 gctcgtcgtc ctggcgcgac tgggtgctcg acctcgacgc ctcccgcccg ctggtgcgca
     2281 gtgccgtcga cgccggaatc accttcttcg acaccgccga cgtctactcg ctcggggtca
     2341 gtgaggaggt gacgggcacc ctgctcggcg agttcttctc ccgccgcgag gactacgtgc
     2401 tcgccaccaa ggtcttctcg accatgagcg accgcccgaa cgaccgtggc ctgtcgcgca
     2461 agcacatcat gtccagcatc gacgcctcgc tgcgccggct cggcaccgac cacgtcgacc
     2521 tctaccagat ccaccgcttc gactccgaga cgccgatcga ggagacgatg gaggcgctgc
     2581 acgatgtcgt gaaggcgggc aaggcccgct acatcggcgc ttccagcatg tacgcctggc
     2641 agttcgcgaa ggcgcagtac acggccgatc tggggggctg gacgcgcttc gtctcgatgc
     2701 aggaccacta caacctgatc taccgggagg aggagcggga gatgcacccg ctctgcctcg
     2761 accagggcgt cggggtcatc ccgtggagcc cgctggcccg cgggcggctg gcccgcgtcg
     2821 cggagcagcg ctccaccacc acccgctcgg ggagcgaccc gatcggcgac cgcatgtacg
     2881 tcgaggccga cgacgccgtg gtgcacgccg tcgccgatgt ggcgaaggcc cgtgacctgc
     2941 cgctcgcgca ggtggcactg gcgtggatgc tccaccagga agcggtgacc gcccccattg
     3001 tcggggccac caagccgggg cacgtcgagg acgcggtggc cgctgtggac gtcgagctgt
     3061 ccgacgacga gatcgccgcg ctcgaggcgc cctacgttcc gcaccccgtc ctcggccacc
     3121 agtagtcgcc gagcgcgcga catggacaca tgtggggatc gccgcgcctc taccactgca
     3181 cgcgagacgg gtgcgcgagg gcgcgcggct ccttgcctgt gcccttgcac gcgccacagg
     3241 tccgccagtg cgcggcctcc ccccagtcgc ggtgccgacg agggatcccc tcgcacgcat
     3301 ggcacagcgt ccacggccac acctggaccg tgaccaggta caggatcacc ccggcgacca
     3361 tgccgacgat cgcgctgccg aaatacgtac ccgcgcccac gaaccccacc gcgaaccaga
     3421 tcgcgaacgc tttcacgggt tcctcacggg actggcgcaa gcgcgcctgg ccactgatcc
     3481 catcttcacg ctgttggttc ctctctggac gtggcgccgg acgggcgcgc cactccaggt
     3541 cgcagatgtt ccagttcgcg ttacggctcg gcggacctgg cgtcgtggac ggacccttct
     3601 gtcgcgcttg cgggttgtgc cctacacccc cgtgcgaaga tcgctccgca gggctctgag
     3661 ctgcctaaac ccgggttctg cacctatgct ctccccatgg acacagcacg ctgttctgct
     3721 gccgtccgga acggccacac gtgtcgatgt cgcgggcgga ggtggcggtg acggctcggg
     3781 cggtcgtcat cggcgcgcct tcgtccggga gcgggaagac gaccgtcgcc accgggctga
     3841 tggcggcact gcaccggcgc gggaccaccg tggccccgtt caaggtcggc ccggactaca
     3901 tcgaccccgg ctaccacact ctcgccaccg ggcggccggg ccggaacctg gacccggtgc
     3961 tcgtcggggc cgagcgcatc gcgccgctcg cgcgccacgg tgcggccggc gccgaggtcg
     4021 ccgtcgtcga aggggtgatg ggcctcttcg acggtcgcct cgccgacgga cacggctcga
     4081 cggcgcaggt ggcgggcctg ctcggggctc cggtggtgct ggttgtggac tgccgcgggc
     4141 agtcgcgcag cctggcggcg ctgctgcacg ggttccgatc gttcgacccg tccgtgcggc
     4201 tcgccggggt cgtcctcaac cgggtgggca gcgcgcggca tgaggcggtg ctccgagcgg
     4261 cggccgacga ggccgggcta ccggtgctgg gggcgttgcc ccggcacgac gcgctggccg
     4321 tgccgtcgcg acacctcggg ctggtcacgg ccgccgagca cggacccgca gcggtcgcgg
     4381 ccgtggacgc gatggccgag ctcgcggcca cccacgtcga cctggacgcc gtgctgcggc
     4441 tcgccgagcc gctgccgccg gggccggcct ggtcggccgc tgacgcggct cgtgcgcgga
     4501 tacgagtgcc ggagcactcg tatccgcgca cggccgtgtt cggcggcccg gcgttcacct
     4561 tcgggtacgc ggagcacgtg gagttgctgg cggcggcggg tgcggaggtc gtggtcgtgg
     4621 acccgctgcg ggacgaagcg ctccccgacg ggaccgctgc cctggtgctc ccgggcggct
     4681 tcccggaaga gcacgtcgcg gcgctgggcg cgaacgagcc gctgcgggac gcggtcgctg
     4741 cgctcgccgc ctccggcgcg cccgtgcacg ccgagtgcgg cggcctgctc tacctctgcg
     4801 agtcactcga cgacgcgccc atgtgcggcg ttctcccggc cacggctgcg atgaccgggc
     4861 gcctgacgct cggctaccgc gacgccgtcg cgctggaagg ctcctcgctt ttcccggcag
     4921 gcgcacgggt ggcgggccac gagttccacc gctgcgccgt cgccccccgg gccggtgtcc
     4981 caacggtcgg ggctgcgccg gcgtggggct ggcgaagtgg cgagccggag ggcctggtca
     5041 ccgaggggtg ggtggccggc ggggtacatg cctctttcct gcacacgcac cctgcgggcg
     5101 acccggaggc cgtgtcacgt ttcgtggcag cagcggcgga gacgccgggc cggcggtgag
     5161 tcgagcaccg gcacgggtcc ctacgaggcg ggttcggcgg cgacccggat gaggaccttg
     5221 cccgccactc cgctctccac agcccggtgc gcctccgcag cctgctcgag ggtgaagtgg
     5281 tggagcggga gacctgctgc ctccccgacg gggagggcgc catcggcgat cgcggcgttg
     5341 atgtcctcgg cggcggcggt gatgttctcc cggcccacgg tgtagagcag gacgaactgg
     5401 taccgggtgt tgagtcccat gttcctgcgg acgtccacgg tgaaaggtgc gccgccgtcg
     5461 ttcgcgtaga tcgagatcgt gccgcgcggg cgcaggacct ggagatcgag ttcggcgttg
     5521 cgcccgatcg cgacctcgac gacgagatcc acaccgtcgg gagcgatgcc gcggatctgc
     5581 gacaccgcgt cggcgtcggt gtagttgacg acgtgatgcg cgccggcagc cgtcgcgtag
     5641 gcggccttgg tggggctgct gacggtcgtg atcaccgttg ctccggccca acgggccagc
     5701 tggatggcgg cgtgcccgac cgcgccggcg ccgcctgcca ccagcaccac cctgccgtcg
     5761 agagtccccg ggcgcaggcg cgccggccca ccctccgcga ccgtgagagc gcgatgggcg
     5821 gtgacggcgg gaacgccgat gctggcgccc aggtcgaagt cggcgcccgc cgggagggga
     5881 aagacgcgct cggccggcac gacggtgtac tcctgcgcgg taccgctggt cggtcgctcg
     5941 tgcgcagcga gcacgaccca gacccgatca cctaccgcaa cgccgtccac ccctgggccg
     6001 acggcgtcga ctatcccggc gccgtcctgg ttgggcacgc gctcgatcgg atcgggctcg
     6061 ccccccaagg cgccactgcg cgatttccag tccgtcgggt tgaccccgga gacgatgacc
     6121 ttgatccgca cgtcacccgc accgggctcg ggtatctcgc gatcgacgag acggagcacg
     6181 gaggcgtcgc cgggctggtc gtagatgatc gccttcatgg tggtggcctc tcaggtcatg
     6241 gattcggggt ggcatcggcc tggtgtgtca ctcgccgccg gcgtcgacga tgccgacgac
     6301 cgcgcgggca cggccgcgag cgctgccgtg ccggcccgat gtccgaggtg atctcgcgcg
     6361 tcgccacgga ggtgctcacg gtagatcacg tcctccagtc gtacccgccg gtcggattac
     6421 cgtgggagct acccggcgaa cccgtcgggg cgggcccaca tcatccgacg caggaggtgc
     6481 gcggcgtgac gggacgacgg cgcgtgccag gaacggcgac atcgagctcg ccttcgagcg
     6541 ggtcggcccg gctgatggtg agccgctcct gctgatcatg ggcctgggca tgcagatgct
     6601 gttctggcac gacgacctgt gcgccgagtt cacgcgcgcc ggcttcagcc ctgcccggtt
     6661 cgacaaccgc gacgtcggtg cttcgacgca cctcacccac ctcggcgcgc cgccgctgcc
     6721 ggtcgtgatt gcgctgcccc aggccgtcgc cgcctaccgc gtgtccgaca tggccgccga
     6781 cgcggtcgcc gtactcgacg cgctggggtg ggagtccgcc cacattgtgg gcgtgtcgat
     6841 gggcgggatg atcgcgcaga ccgtcgccat cgagcacccg caccgcgtgc ggtcgttgac
     6901 ctcggtgatg tcgaccccgt cgccgaggat cggacggccg acgctcggcg cggccgccgc
     6961 tctggccgcg cggccgggac gcaccgccga ggaggcggcg cagcgggtgg tggacacgtt
     7021 tcgcgtcatc ggctcaccgg gctacccact cgacgaggac tggctgcgca actacgccag
     7081 gcgcgccttc gcccgcagca aggactccac cggcacccgc cgtcagctcg ccgcggtcaa
     7141 cgcctcgccg agccgcgtgg ccgggctgcg cagcgtgcgg gcgccgacgc tggtggtgca
     7201 cggcgaggcc gacccgctgg tcggaccgga ggccggacga gctacggcgg cagcggttcc
     7261 gggtgcccgg ctcgtcacct atcccgggat gggccacaac ctgccgcgcg agctctggcc
     7321 gtcgatcgtg cggcaggtgg cgggcctggt gggctgagct ccgccgggtt cacccacccc
     7381 ggccgagctg gtgcaccacc gacgcggtgc tggcgtagag ctcgcggtac agcccgtaca
     7441 gccggtgata ggtggcggcg agcgcgggct gcggtgtgac ttccgtccgc accgggttcc
     7501 acacgttgat cgacggtgcg gccaccagcc cgccggcgag gaaggcagcg ccgaaactcg
     7561 cgccgatggt gacgctcggt atctgctgcg gcaagccggt cacgtccgag acgatctgcg
     7621 tccagagggc gcctcgcacc ccgcccccga cggcgacgac ccggtcgatc tgcccgcccg
     7681 cctcccggaa gaactcgacg ttgtgccgga cagccagggc cgtcgcctcc agcgccgccc
     7741 ggtacaggtc gcccgcggtg tgctcgagtg tcagccccac gatcacgccc cgtgcctcgg
     7801 ggtcggccac gggcgtccgt tccccggcga agtagggcag catcagcagc ccgcgtgcgc
     7861 cggggcccga acgcccggcg gcgtccagga gcgtggtgta gtccggtgag ccggcgaggc
     7921 ggcgcagcca ggctgtgatg gccccggacg tcgccattcc cccggcgagg ttgcgcgtcc
     7981 cgggaagggc gccgacggtg ctccacagga ccggggacgt acggcgctgc gccaccgtgt
     8041 tgatgaggaa cagcgtggtt ccatacatca gcatcaggtc gccgaccctg tgcgcgccca
     8101 cgctgaccgc ttcggaccag gcgtcgatcg tgccggtgat gaccggggtc ccgacgcgca
     8161 acccggtgac ggctgcagcc tcggcggtca ccgtgcccgc ggcctcgccc ggccaccgca
     8221 gctcgggtag ctccagatcc ggggcgatgg cgcgcaccca cggctcgtac cacgcatgtg
     8281 cctccgtgtc gtacatcggt gagcactggc tggccgagtg atggtcgagc acatacgcgc
     8341 cggtcagctg gtggacaagc caggagctgg gcatgaacaa ccggcgcacc cgcgcgaacg
     8401 tctcgggctc gttctcggcg atccacagca gcttgggccc taccgcctgc gtggacaatg
     8461 cggagccgcc gcgatcgaga atgtcctgcg cgcccagttc gtcctccagg cggcggatct
     8521 gcgcggtggc gcgcatgtcg acgccgtaga ggatcgccgg ccgcaggggc gtgccgaccg
     8581 cgtcggtgac cgcgacgcac gggcccatgc cgctgacgcc caccgctgtg acgtcgacat
     8641 cgcgcccggc gagcagctcc cgggccagca gcacgaactc gctccaccac aggcccgcgt
     8701 ccatctcgaa ccagcccggc tgcgggcacg acacctcgtg ctcccggacg gcggacgcca
     8761 ggatcgcccc gtcgagggtg acgaggacgg ccttgctgct ggaggtgccg atgtcgacgc
     8821 ccagcacgac cccggtcatg gggtctcggg gagcgggacg tcgccacaca ggtggaccgt
     8881 cacgccgagg cgggcgaaca gcgccgcctt ggccagcagc gcccggtcgg ccgtctcggc
     8941 gtcgggcgcg taggcgacct gggcatggtt ggccttgtgc cgggccatga actggttgcg
     9001 cgtgatgccg tgcagcacca cgtgcatgat cggccactcc gggttggtcg ccgcccagcg
     9061 gcgctgcgtc tcctcctccg gcagttcgac agccgtcgcc cggccgatgt ccaggtgcag
     9121 tccgccctcg gcgatgtaca cgcgcgacca gacgatctca cccggcttgg aaacgcctct
     9181 gatggtgctg cctccggcgg ggaagaacac cgggctctgg cgccagccga cagcatcggc
     9241 gtagccgttt cgaaggtgtg agggcggcac cgacccggag atctcgaaca cccacacgaa
     9301 ctcgtcgccg aactgctcgc cccagcggac gtcgtgcagc gtcgtggccg ggtccagccc
     9361 catggccgac cacacccggt tggtcagcag cgcgtcgacg gccacgccct cgtcggcctc
     9421 gttgaagtgg gggagtgcca ggccctccca cagcacgcgg ctcccgtcgc ggctggtgac
     9481 cggaggccgc tcgacgttgt tgagcaggcc ctcggccagg tccgatgccg gaaccaggtc
     9541 cttcaggccc tgctggtact ggatccccac ggcgtccagc ccgaagtcgt cgctgatccg
     9601 cagcgcggcg atgtacatct tgtactgcca gctgagctgg gcctcggtga gctcggtggc
     9661 atcgtccgtg ccggtcagga agcgcatgcc ccgctccgcc agccagctcc ccacggcagc
     9721 tgcgtcctcg tcgggcacgc ggagcatctc ggcgtacagg gcgctctgcg agagccgctc
     9781 cttgaatatg ccgagcgggt tgagcagctc gtcgtcgatg atcgcgttgt acatccccat
     9841 gcagccctcg tcgaagacgc cgatgatcgc cttctcggtc tgcagctggt cggccagcgc
     9901 ctcgcccagt tccttctcgg ggatggcggg cagcgcaggc agcgggcgga cgtggttcgc
     9961 gtcgtgctcg atccggcccg tctcgaccca gctccgcaag ccgtcccgga accacgagtc
    10021 ggtgaagtcg acgctccaga gcgtggagta cggcgtgccc atcttcgtca ggcccgcatt
    10081 cagtcccagc agcccgacca ggccgggcca gtcgcccacg aagttggcga cggtgaggat
    10141 cgggccgcgg tgcgtgcgca ggccggcgag cacgtggtgg ctgtactgcc agacggcctc
    10201 ggccacgacg aggggcgcgt ccggcgggat ggacgcgaag accgtcaagc ccatccgctg
    10261 gctgtcgatg aagccgtgac ccttctccgg atcgaccgga ttggcgcggc gcaacgtcca
    10321 gcccaggtcc gcgaacgccc gcgtcagcga ctcctcgacg ccggcctgcg tgggccagcc
    10381 ggcgacgttg gccgacgggc gaaggtcgcc actggcgatc aggaacgccg tcgcgggcgg
    10441 ggtcgtgccg tgctcgcgga cctccggcag cgcgtacgtg gtcatgggtg actccttcgt
    10501 cgtgatcacc gcgtgcccat ccggtcgcag cacgggccgt gccgcggagc ttatccagaa
    10561 atcgattact cacgtcaagc gcggacggcg ttttgcctgg acacggtcga tatgggcaca
    10621 gtaccgtctg ccagcaaacg tttacccgac gacgcagccg ggaaggccgc agcgacaggg
    10681 agaacggccg tcgtcggaag cggggcccgt accgtgcggg cctcgtcccc gctggagggt
    10741 ggggcgcgag gaggaaggtc ggcggccgaa gtggtcaaga tcaatgatgt tgcgcgggaa
    10801 gccggggtgt cggctgccac ggtgtcccgc accctcaacg gccggtcctc cgtcgacccg
    10861 gtgctcgccg ccagggtccg cgaggccgcg agccgcctcg gttaccggcc caacggtgtg
    10921 gcccgcagcc tgcggcgcca ggtcaccgac gtgctggccc tgatcatcac cgacgtgagc
    10981 aaccccttct tcaccgcgat cacccgcggg gtggaggacg tcgcgcagag caggggcctc
    11041 tcggtactgc tgtgcaacag cgacgaggac cccggcaagg agtcgaccta cctccacgtc
    11101 gcggagcagg agcaggtggc cggggtgatc ctctccccgc accgcaccgg cagcgatgtc
    11161 gcccatctgc ggcgcgcgtc catccccatg gtcgtcatcg atcgcccgct ggacgagccg
    11221 gtggactcgg tgatggtgcg ttcgcgcgag ggtgcgcggg cagcgaccaa tcacctcatc
    11281 gacgcggggt ggacccgccc ggcctgcatc accgggccgg aggatgcctg gacggcccag
    11341 gaacggctcg agggctacct ggaggcggtg cgcgagcacg gggagctggc cgagctgtgg
    11401 gtccacgcgc cgttccggca gcagggcggc atgaccgggg cggcccggct gctggatgcc
    11461 cccgagccgc cggacgccct gttcgtggcc aacgcccaga tggcgctggg tgttctggag
    11521 gaggtccgcc gccgcggact tcgggtcggc gtggacctcg gggtgatcac cttcgacgac
    11581 gcgccgtggg cgcccttcat cagcccgccg atgtccgtcg tggcgcagcc ggcctacgac
    11641 gtcggcgcgc aggctgctca gctcctgatg gagcgggtca agggcactgc gcccgacgcg
    11701 ccgcggcacc tgtggttgtc gaccatgctc atcgtccgcg agagctcgcg acgccacggc
    11761 gcgggctgag ccgccgcgca gcgcaggccg tgccgtaccg gtggttgaca cgccgaaagt
    11821 gcagacccta aggtcagcaa tcgatttcta agatcttgtt ccggcaggcg agcaatcgat
    11881 ttctgcgcca gaccgacccg cccacgccgc ccggtgctct actcaccagc gctcgacgag
    11941 gagtagccat gccgtcgtac tcatagtgga tgctcgagta cggccactgc cacgcgcagc
    12001 cattgggatg catcttctac ggcgagtggt ccggccggac gcgcgagttc gcctacagct
    12061 acgtgtacgt cgagggcgcg ggccacaaga tccttgtcga cgtcggtcac gacatccggt
    12121 ccaccaacaa ggcctggaac gacgacaacg acatcgtcga ctaccaggct cccgacgccg
    12181 tcctcgcgaa ggtgggcgtg cgacccgagg acatcgacac ggtggtcctc acgcacgcgc
    12241 actacgacca cgcgggcgcc gcccgctggt tccccaacgc ccgcttctac ctgcagcgcc
    12301 gcgagttgga gagctcgcgc tgggcactcg ggcagtcgcc gctctacgac tcgatcatcg
    12361 cggcgctcga tcccgccgac gtcacgatgc tcgaggagat ggcgcgtgac ggcaggctcg
    12421 tgctgctcga cggggcgctg cagctgttcc ccggcctcga gatccggacg gcgtgggaca
    12481 cgcacacgca gggtggtcag tacgtcgtga tcaccgacca cgacgatcac cgctgggtgg
    12541 tcaccggcga cgcgatgtac agctacgaga acgccgaggg catcgacggt tcgggccgct
    12601 atgtgaacat cggcttcggc ggtggcagcg gctgggacgg gctgcgcctg atcgatgaga
    12661 tggtcatgac tgccgggagc acagaccggc tggtcatcgt gcacgagtcg gcgacattcg
    12721 ccaggaaccc gtcgcgccgc tacgacgacc gcctatcggt cgcagagttg ctgctggcac
    12781 ccggacagcc ctctcgtatc ggggcgcccc ccgccgggtg cgaccccgac ctctccccgg
    12841 cagcagtgct gccctccccc gaagactcgt gaaggagtca gcgatgccca ggaacagagc
    12901 agttctcgcc atggccggcc tactggtgct gggcctcgcc gcctgcagcg tcccctccgg
    12961 caacggagct gcgccgacag gcggcacgag caccagcaag gtcgtcggga tcgtgacgat
    13021 cagcgccacc gacgccaaca acgcgcgcgt catcgccgct gtgaccgagt ccgccaaggc
    13081 cgcggggtgg acggtcgacg tcgtggacgc cgcgggcaac gcggaccagg cgaactcggc
    13141 gatgcgcaac ttcgtgtcca agggcgacgg gatgatcttc gacctggtgt tccccacgtc
    13201 ctcgctcggg gccggtctcg cctccgcgag gcaggcaggc gtgcccgtcg caacctgggg
    13261 agggggtctc ggcaccggag tcgtcatgac caccggggac ggcgccccgt tcgcccagcc
    13321 cgccacgcag aagatggtcg acgacctcgg tgggagcggg tctgtgctcg ccctcaccta
    13381 ccgggcgggc caggtgtgcc gcgaccgcga gggcgtcttc gaccagatca tgtcgacgaa
    13441 cccgggtgtg caggtctcca aggaggaggt cacgattccg ggattcctgc aggacggctc
    13501 caagtacgcg acggcgtggc tcgccggtca cccgcaggga tcgggaccgc tggccatctg
    13561 gggctgctgg gaggacccca cgctcggcgc gatctcagcc ctgaagcagc agaaccgcac
    13621 agacgtcaag acctacggga tcaacggttc cagcaccgcg atcaaggccg tgcaggacgg
    13681 gagcctcacg gcgacggtct acgaggacgg cgcagccgag ggaaagacga tgttcgacac
    13741 gaccatgcag gccatccagg cgggcgccgg ctggcagccc aagaccgtga acgtgccggg
    13801 gatcctcgtg actgcgcaga ccgtcggcca gttcgtccag gaccaccccg aagcgctgaa
    13861 gtgagagcat ccgtcccctc ggcggatccg ctcatccgga tcacggacgt gagcaaggca
    13921 ttcggcggcg tccaggcgct cgccggtgtg tccttcgaga tccggccggg tgtcgtgcac
    13981 ggcctcgtcg gtgcgaacgg cgccggcaag tccaccctga tccgctgcct ggcgggcatc
    14041 gggctgcccg acagcggctc gatcgaggtc gacggcatgc cggtctcgat ctccagcacg
    14101 gatgatgcga cgcgcctcgg tctggcgttc atccaccagg agatgagcct catcccgggc
    14161 tgggacgtgc tccgcaacat ggccctcggc gtccggccac ggacgcgctt cggcgtgatc
    14221 gactggcgct ccacccggca gcgtgccgcg gaggtggccc ggcggctggg catgcgcttc
    14281 gggctgggct ctccgatcgg cgacctcagc acagcggacc agtggttggt cctcatcggc
    14341 cgggccctca tgcgcgacgc gcggatgatt gcgatggacg agccgacggc gtccttgtcg
    14401 gtggaggagg caacgcggct gcacggcatc gtgcgcgagc tcgccgcagc aggcacggcg
    14461 atcatcttcg tctcgcaccg actggacgag gtgtctgcgc tctgcgagga cgtcaccgtc
    14521 ttcaaagacg gccgcgtcac caagcgggtg gtgggggagc cgacgagtaa gtctgacctc
    14581 gtccgcgcca tcgtcggccg ggacctggag atccctgagc acggccagga ggcggtcaga
    14641 gccgggcgca cggtgctcga ggtccgcgat ctctccgacg gtgcgcgcgt gcaggacgtc
    14701 tccctgacgg tccacgaggg agaggtggtg ggcctgggcg gactggtcgg agccgggcgc
    14761 accgagctgg tcaagatgat ctacggcgcc gagcgctcgg ccggcggtga ggtgctcctg
    14821 gacgggcaac gcgtttcctt ccggtcgcct gccgacgccg tgcgcgccgg gatgggcctc
    14881 gtgccggagg agcggcgctc ggagggcctg ttcctggatc ggacgatcga cttcaacatc
    14941 aatgtcgcca cgctcgggtc gctccggagg tcgcgctggc tgcccctgct gcggtcggcg
    15001 gcggggcggc gccgtgccca gagcgtcgcc gacctcgtgg ccgtcaaggc ggagtccgtg
    15061 aacgtgccgg tcggctcgct gtcgggtggc aaccagcaga aggtcgcgat cgcgcgctgg
    15121 ctgctcgaga cgccgcgtct gctcatcctc gatgagccgt cgcgcggggt ggatgtgggc
    15181 gccagggccg aggtgcaccg ggtcgtccgg gagctcgcat cgcggggcgc ggccgtgctc
    15241 gccgtctcct cggacaacga ggaactggtg gcgctgtgcg accgggtcct cgtgatggcc
    15301 gagggccggg tcaccggcga gctgtccggc acggagatca cggctgaccg catcgtgtcg
    15361 ttgagcttcg cgaggggctc cggagtaggc gcagcagcag aaggagcaac cgcatgagca
    15421 ccgagacagc cggacccgtg gacgtggcgc cgccgcccgg tgcgccgcgc ccgcaccggc
    15481 cggcgagccg gatcgggctg atgatcgtgt cgaagtacgg cacgctcatc gcgatgggca
    15541 tcatgatcgt catcttcggc acctcggtgc cgaacggagc gttcctgacg tcgaacaacc
    15601 tgctcaacat cctcaaccag tcggccctca cggcgatcat cgcgttcggg ctgacgatgg
    15661 tgctcgtcgt gggcgagttc gacctcagca tcgggtacgc cgccagcttg gccggcgtgc
    15721 tggtgaccgg tctgctggtc aaccagggtc tggcgctccc ggtggccatc ctgctcaccc
    15781 tcctgctggg cgcggccatc ggcgcggtca acggccttct ggtaaccaag gcgcgggtga
    15841 atgccgtggt ggcgaccctc ggcgtcggca ccatgctgat cgggctgtcg tacggataca
    15901 cagctggatc gccgatcatc gcggtgccga cctcgttcac caacatcacg ttgggcgcct
    15961 tcatcggcgt ccagaacccg atctggttca tggtgatcgt gctcgggttg ctgtgggtgc
    16021 tgctgaaccg cacgccgctg ggccagcgca tccaggcggt cggcgccaac acggaggccg
    16081 cccggctggc aggcatccgc accgaccggg tcaaggtggc cgccttcgtc
//
LOCUS       DASRSC010000259         7840 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2557580,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000259 DASRSC010000000
VERSION     DASRSC010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2557580"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VFE39_12685"
     CDS             complement(<1..563)
                     /locus_tag="VFE39_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HET6259500.1"
                     /translation="MTVRVGVNGFGRIGRNFWRAVDAQRTAGTSDIEIVAVNDITDNA
                     TLAHLLKYDSILGRLPYDVTSTDEEIVVDGKGFKGLAVRDPAELPWKDLGVDVVIEST
                     GLFTKREAAAKHLDAGAKKVVISAPATGEDLTVVKGVNDGEYDGSQNVVSNASCTTNC
                     LAPLAKVIDDLLGIEKGLMTTIHAYTQ"
     gene            complement(799..1698)
                     /locus_tag="VFE39_12690"
     CDS             complement(799..1698)
                     /locus_tag="VFE39_12690"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6259501.1"
                     /translation="MPDTRYARNGDVEIAYEDLGGAGGDPLLLVMGLGVSRFWWQPGL
                     VAELVGRGFHVVAYDQRDAGESTRFPDREAVHPVVALFRRQPPAYTAEDMADDAIAVL
                     DALGWDSAHLFGHSMGGLLVQRIAIRHPGRVRSITVSGAMPSDAGGLRLLRYLRPAFL
                     VRASRLRFPEGRAGDIAASLALARLVASPGYPFDEAAALAVAERDVVSGVRDSRAQSR
                     QIGAKWSGGRLAELRLPALVLHGTDDQIGRPAAGRDTAAAIAGARLVLLPGVGHDLPR
                     ALWTQVADEVRLVADRATAMSAG"
     gene            complement(1771..2754)
                     /gene="whiA"
                     /locus_tag="VFE39_12695"
     CDS             complement(1771..2754)
                     /gene="whiA"
                     /locus_tag="VFE39_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET6259502.1"
                     /translation="MAMTASVKDELSRLVVTKPCCRRAEVASLLRFAGGLHIVGGRVV
                     VEAEVDTGSVARRLRREIHELYGHTCEAHVITAGGLRRGARYVLRVVRDGEGLARQTG
                     LLDARGRPVRGLPPPVVSGGLCDAEAAWRGAFLAHGSLTEPGRSSSLEVTCPGQEAAM
                     ALVGAARRLGVTAKWREVRGADRVVVRDGDAIGALLTRMGAHESVMAWEERRMRREVR
                     ATANRLANFDDANLRRSARAAVAAAARVARALEILGDDVPQHLLAAGRLRAEHTQASL
                     EELGQLADPPMTKDAVAGRIRRLLHMADKRAADLGIPDTESAVTADMLEEA"
     gene            complement(2798..3844)
                     /gene="yvcK"
                     /locus_tag="VFE39_12700"
     CDS             complement(2798..3844)
                     /gene="yvcK"
                     /locus_tag="VFE39_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010310745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HET6259503.1"
                     /translation="MTSGASAVALGGGHGLHATLSALRLLADGGRIADSVTAVVTVAD
                     DGGSSGRLRRELGLLPPGDLRMALAALASESPAGRRWGALVQHRFGGTGALAGHAVGN
                     LLLAGLMDVLGDPVAALDEVGALLGLRGRVLPMSCEPLDIEAEVTGLGEQPAEPHRIR
                     GQVAVASTPGRVRQVWLRPDRPRACPEAVEAVRAADVLLLGPGSWFTSVLPHLLVPEL
                     RDAVIASRARRVIVLNLAPEPGETAGFSPEQHLAVLSQHAPALRVDAVVADVAAVPVP
                     DRLHRAAAVMLSPGGRVHLAPVAAADPAVPRHDPASLAAALGAVLAGFDPRPASPDAV
                     PVPSVPVAAERRRT"
     gene            complement(3856..4746)
                     /gene="rapZ"
                     /locus_tag="VFE39_12705"
     CDS             complement(3856..4746)
                     /gene="rapZ"
                     /locus_tag="VFE39_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET6259504.1"
                     /translation="MTSSSTIRPAGIEVALVSGLSGAGRSTAAKVLEDLGWFVVDNLP
                     PELIATMVQLGARARGEITRIAVVMDVRSRAFSADLGAVIKDLDARGYKPRLLFLEAS
                     DAVLVRRFEQVRRSHPLQGDGRLVDGIAAERELLRPLRESADLVVDTSTVAVPQLRGT
                     LERAFGPESAQVTRITLVSFGYKYGLPMDSDLVVDVRFLPNPFWIPELRDQTGRDGPV
                     RDYVLSQDGADEFITRYLELLRLVGAGYRREGKRYLTVSVGCTGGKHRSVAISEEIAR
                     RLEGAEGVTVNVAHRDLGRE"
     gene            complement(4743..6848)
                     /gene="uvrC"
                     /locus_tag="VFE39_12710"
     CDS             complement(4743..6848)
                     /gene="uvrC"
                     /locus_tag="VFE39_12710"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET6259505.1"
                     /translation="MPQPASSDPARYRPAPGSIPEAPGVYRFSDQHGRVIYVGKAKSL
                     RQRLNSYFADLAGLHPRTRQMVTTAARVEWTVVATEVEALQLEYNWIKEFDPRFNVRY
                     RDDKTYPVLAVTMNEEFPRLHVYRGPRRKGVRYFGPYAHAWAIRETLDLLLRVFPART
                     CSAGVFKRHGQIGRPCLLGYIDKCSAPCVGRVDADEHRDIVEDFCDFLSGRTDHLVRQ
                     LERKMADASEGLEFERAARLRDDIGALRRAMERQAVVLGDGTDADVVAFAEDELEVAV
                     QVFHVRGGRVRGQRGWVIDKVEPTDTAQLVERFLTQFYGEQAALAGSADDAHQPVPKE
                     ILVPELPAEVDALVDWLSRLRGSRVQVRVPQRGDKRALAETVARNAAEAFTQHKLRRA
                     GDLTARSAALQEIQDALGLDSAPLRIECVDVSHVQGSNVVASLVVFEDGLARKSDYRR
                     FEIRGGAEGGDVAAVAEVVRRRFRRYLAETGSDRDGTEGDARGNGVQDQSEGSGIASP
                     ASTPARPGIDPETGRPRRFAYPPNLLVVDGGQPQVEAAADVLADLGVNDVAVCGLAKR
                     LEEVWLPAEPDPVILSRTSEGLYLLQRVRDEAHRFAITYHRQRRSKSMISSELDAVPG
                     LGPSRRAALLKHFGSLRKLKAADVDAIAALPGFGPRTAAAVLAALHSADRTGQGGPTE
                     SDRIDRTDRTETDAEVPAT"
     gene            6996..7721
                     /locus_tag="VFE39_12715"
     CDS             6996..7721
                     /locus_tag="VFE39_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HET6259506.1"
                     /translation="MGVDKLDTVRKLLVKAERAATGAEAETYTAKAMELMARHGIDAA
                     LLAAARPDTDRVGPQRIEMQDPYSAGKARLLGWTASALGCRWVLHGAWGGKVAAVTVF
                     GHASDRERVELLYTSLQLQASTQLVRVRPAWPGESVAAYRRSWLHGFAVEVHRRLSSA
                     EARAAASAAEPAADGPGVALVLADRRERVDRAYAAAFPRLGTARRTVLSGSGFAAGAR
                     AGERADLGRATGIDRGRRRALGA"
BASE COUNT         1109 a         2923 c         2766 g         1042 t
ORIGIN      
        1 tcctgggtgt aggcgtggat cgtggtcatc agacccttct cgatcccgag caggtcgtcg
       61 atgaccttcg ccagcggggc gaggcagttc gtggtgcagg acgcattcga cacgacattc
      121 tgcgagccgt cgtactcccc gtcgttcacg cccttgacga cggtgaggtc ctctccggtg
      181 gccggcgcgg agatcacgac cttcttggca ccggcgtcga ggtgcttggc cgccgcctcg
      241 cgcttggtga acaggcccgt cgattcgatg acgacgtcga cgccgaggtc cttccacggc
      301 agctccgcgg gatcccgcac cgcgaggccc ttgaagccct tgccgtcgac gacgatctcc
      361 tcgtccgtgg acgtgacgtc gtagggcagc cggcccagga tcgagtcgta cttgagcagg
      421 tgtgccaacg tggcgttgtc ggtgatgtcg ttcaccgcca cgatctcgat gtcactggtg
      481 cctgcggtgc gctgcgcgtc caccgcccgc cagaagttac ggccgatccg accgaacccg
      541 ttcacgccaa cccgcaccgt cacggtgcct ccttgatctc gaaggacgac ccactcgaac
      601 cggtcaccac cctatcggcc ggatgagcag gcgcgcgggc tagtgttcgg aatcgatcca
      661 ccttgttcta ccggcggcta ccccctcggg caacgccctg cgcgacgtcg cgcaagcacc
      721 tccgatcctt ccggcaccgg accctggacc acgccggcgc cctccgccga ccggcgagcc
      781 cggacccctc cgagccactc acccggcgct catcgccgtg gcgcggtcgg ctacgagccg
      841 cacctcgtcc gccacctggg tccacagcgc gcgaggcagg tcgtggccga cgcccggcag
      901 gagcaccagc cttgcgcccg cgatggccgc ggccgtgtca cgacctgcgg cggggcgtcc
      961 gatctggtcg tccgtgccgt gcagtacgag cgcgggcaga cgtagctcgg cgaggcggcc
     1021 gccgctccac ttcgcgccga tctgcctgct ctgtgcgcga ctgtcccgca cgccgctcac
     1081 gacgtcgcgc tcggcgacgg cgagcgccgc ggcctcgtcg aacggatagc cgggcgaggc
     1141 gacgagcctg gcgagcgcga ggctcgcggc gatgtcgccg gcgcgcccct ccgggaaccg
     1201 caaccgcgac gccctcacca ggaaggcggg acgcaggtag cgcagcaggc gcaggccgcc
     1261 cgcgtcactg ggcatcgctc ccgagacggt gatgctgcgc accctgccgg ggtgccggat
     1321 ggcgatgcgc tggacgagca ggccgcccat cgagtggccg aacaggtgcg ccgagtccca
     1381 gccgagggcg tcgaggaccg cgatcgcgtc gtcggccatg tcctcggccg tgtacgccgg
     1441 gggctgtcgg cggaacaggg cgacgaccgg atggacggcc tcgcggtcgg ggaagcgggt
     1501 ggactcgccc gcgtcgcgct ggtcgtaggc cacgacgtgg aagccgcggc cgaccagctc
     1561 ggccaccagg ccgggctgcc accagaaccg cgacacgccc aggcccatga ccagcagcaa
     1621 cgggtcgcca ccggcgccgc cgagatcctc gtaggcgatc tcgacgtcgc cgttgcgagc
     1681 gtaccgggtg tcgggcatcc cagcctcctg acattgcgaa cactgtgtgc agtgccaggt
     1741 tacctctttg cgaacagtgt ccgcaaagcg tcaggcctcc tcgagcatgt ccgcagtgac
     1801 cgcggactcc gtgtccggga tgcccaggtc ggctgcccgc ttgtcggcca tgtgcaggag
     1861 cctgcggatc cggcccgcca ccgcgtcctt cgtcatcggc gggtcggcca gctgacccag
     1921 ctcctccaac gacgcctgcg tgtgctcggc ccgcagcctg cccgcggcca gcaggtgttg
     1981 cggcacatcg tcaccgagga tctccagtgc gcgggccacc ctggccgcag cagccaccgc
     2041 ggccctggcc gaacggcgca ggttagcgtc gtcgaagttg gcgaggcggt tggcggtggc
     2101 acggacctcg cggcgcatcc gccgctcctc ccacgccatc acgctctcgt gcgcgcccat
     2161 ccgcgtgagc aaggccccga tggcgtcgcc gtcgcgcacc acgacgcggt cggcgccgcg
     2221 cacctcccgc cacttcgcgg tgaccccgag cctgcgcgcg gcgcccacga gtgccatcgc
     2281 cgcctcctgc cccgggcagg tgacctccag ggaagagctg cgcccaggct cggtgagcga
     2341 gccgtgcgcg aggaacgccc cccgccacgc cgcctcggcg tcgcacagac caccggagac
     2401 gacgggcggc ggcaggcccc ggaccggacg gccgcgggcg tcgagcaggc cggtctggcg
     2461 ggccagccct tcaccgtcgc gcaccacccg cagcacgtag cgcgcgccac ggcggagccc
     2521 gcccgcggtg atcacgtgcg cctcgcaggt gtgcccgtac agctcgtgga tctcccggcg
     2581 gagcctgcgc gcgacggagc cggtgtcgac ctcggcctcg accacgaccc ggccgccgac
     2641 gatgtgcagg ccaccggcga atcgcagcag ggacgcgacc tcggcgcgtc ggcagcaggg
     2701 cttcgtgacg acgagcctgc tcagctcgtc cttgaccgat gcggtcatcg ccatgtcgac
     2761 ctcctgacgg gatcgggcag gggagcggaa gtgacgttca cgttctcctc ctctcggcag
     2821 caaccggtac cgacggcacg ggcaccgcat cgggcgaggc cggtcgcggg tcgaacccgg
     2881 ccagcacggc acccagcgcg gccgcgagcg acgccgggtc gtggcgcggc accgccggat
     2941 cggccgcggc gaccggggcc aggtggaccc gaccgcccgg ggagagcatc accgcggccg
     3001 cgcggtgcaa ccggtcgggc accggcaccg cggcgacgtc ggccacgacc gcgtcgaccc
     3061 gcagcgccgg tgcgtgctgg gagagtacgg ccaggtgctg ctcgggtgag aacccggcgg
     3121 tctcccccgg ctccggtgcg aggttgagca cgatcacgcg gcgggcacgg ctggcgatca
     3181 ccgcgtcgcg cagctcgggc accagcaggt gcggcaacac gctggtgaac cacgatcccg
     3241 gccccagcag cagcacgtcg gcggcacgga cggcctcgac cgcctcgggg catgcccgcg
     3301 gccggtccgg tcgcaaccac acctgccgca cccgccccgg cgtggacgcg actgccacct
     3361 ggccgcggat ccggtgcggc tcggctggct gctcccccag gcccgtgacc tcggcttcta
     3421 tgtcgagcgg ctcgcagctc atcggcagta cgcgcccccg cagcccgagc agggcgccca
     3481 cctcgtccag cgcggccacc gggtcgccga ggacgtccat gagcccggcg agcagcaggt
     3541 tgcccacagc gtgcccggcc agcgcgccgg tgccgccgaa gcggtgctgc accagggcac
     3601 cccaccggcg gcccgctgga ctctcggagg ccagcgcggc caacgccatc cgcaggtcac
     3661 cgggcgggag cagccccagc tcacggcgga ggcgccccga ggaacccccg tcgtcagcca
     3721 ccgtgaccac cgcggtgacg ctgtcggcga tccggccgcc gtcggcgagc agccgcagcg
     3781 cggacagggt ggcgtgcaac ccgtggcccc cgccgagggc cacagccgac gccccggagg
     3841 tcacgagccc ggcgctcact cgcgccccag gtcgcggtgg gcgacgttga ccgtgacgcc
     3901 ctcggcgccc tcgagcctgc gggcgatctc ctcgctgatg gcgacgctgc ggtgcttgcc
     3961 acccgtgcag ccgaccgaga ccgtcaagta ccgcttgccc tctcggcggt agccggcccc
     4021 gacgagccgc agcagctcca ggtagcgggt gatgaactcg tcggcaccat cctggctcag
     4081 cacgtagtcg cggacgggtc cgtcccggcc ggtctggtcg cgcagctcgg ggatccagaa
     4141 cgggttgggc aggaaccgga cgtccaccac gaggtccgag tccatcggga gaccgtactt
     4201 gtagccgaac gacaccaacg tgatacgtgt gacctgcgca gactccggac cgaacgcgcg
     4261 ttccaacgtg ccgcgcagct gcgggaccgc cacggtcgag gtgtcgacga ccaggtcggc
     4321 gctctcccga agcgggcgca acagctcgcg ctcggcggcg atgccgtcga cgagcctgcc
     4381 gtcgccctgc agggggtggc ttcgccgcac ctgctcgaac cggcgcacca gtaccgcgtc
     4441 ggacgcctcc aggaacagca gccgtggttt gtaaccccgc gcgtccaggt ccttgatgac
     4501 ggcgccgagg tccgcggaga acgcgcggct tcggacgtcc atcaccaccg cgatccgggt
     4561 gatctcgccg cgggcgcgcg cgccgagctg gaccatcgtg gcgatcagtt cgggtgggag
     4621 gttgtccacg acgaaccagc cgaggtcctc cagcaccttc gccgcggtgc tgcgaccggc
     4681 cccggacagc ccgctgacca gagcgacctc gatgccggca ggccttatcg tggatgatga
     4741 cgtcacgtcg ccggcacttc cgcatccgtc tcggtccggt cggtccggtc gatccggtcg
     4801 gactcggtcg ggccgccctg gccggtccgg tccgcggagt gcagcgcggc cagcacggcg
     4861 gcggccgtcc gcggcccgaa gccggggagc gccgcgatgg cgtcgacgtc ggcagccttc
     4921 agcttccgca gcgacccgaa gtgcttcagc agcgctgcgc ggcggctggg cccgagccca
     4981 ggcacggcgt ccagctcgga gctgatcatg ctcttcgacc ggcgctgccg gtggtaggtg
     5041 atggcgaagc ggtgcgcctc gtcccgcacc cgctgcagca ggtacaagcc ctcgctggtg
     5101 cgcgacagga tcaccgggtc gggctcggcc ggcagccaca cctcctccag ccgcttggcc
     5161 agcccgcaca ccgcgacgtc gttgacaccg aggtcggcga gtacgtcggc ggccgcctcg
     5221 acctggggct ggccaccgtc gacaaccagc aggttgggcg ggtaggcgaa ccgtcgtggg
     5281 cgcccggttt cgggatcaat gccgggacgt gcaggggtac tggcggggga agcgataccg
     5341 gaaccttcgc tctgatcttg aacgccgtta ccccgcgcgt cgccctcggt gccgtcgcga
     5401 tcactcccgg tctcggcgag atagcgccgg aaccggcgcc tgaccacctc ggcgacggcg
     5461 gcgacgtccc cgccctccgc gcccccgcgg atctcgaacc ggcggtagtc cgacttccgc
     5521 gcgagcccgt cctcgaacac cacgagcgac gccaccacgt tgctgccctg cacgtggctg
     5581 acgtcgacgc actcgatgcg cagcggcgcg ctgtcgagcc cgagcgcgtc ctggatctcc
     5641 tgcagcgccg ccgaacgcgc cgtgaggtcg cccgcccgcc ggagcttgtg ctgggtgaac
     5701 gcctccgccg cgttgcgcgc cacggtctcg gccagagcac gcttgtcgcc gcgctgcggc
     5761 acacggacct gcacccgcga cccacgcagc cgcgagagcc agtcgacgag cgcgtcgacc
     5821 tctgccggga gctccgggac gaggatctcc ttcgggaccg gctggtgggc gtcgtccgcg
     5881 gatccggcca gtgccgcctg ctcaccgtag aactgggtga ggaagcgttc cacgagctgg
     5941 gcggtgtcgg tgggctccac cttgtcgatc acccagccgc gctggccgcg gacccggccg
     6001 ccccgcacgt ggaagacctg gaccgcgacc tcgagctcgt cctcggcgaa tgcgacgacg
     6061 tcggcgtcgg taccgtcgcc cagcaccacg gcctgtcgct ccatcgcccg ccgcagcgca
     6121 ccgatgtcgt cgcgcagccg ggcggcccgc tcgaactcca gaccctccga cgcgtcggcc
     6181 atcttgcgct cgagctggcg gacgaggtgg tcggtgcggc cggagaggaa gtcgcagaag
     6241 tcctcgacga tgtcacgatg ctcgtcggcg tcgacgcgcc cgacacacgg cgcggagcac
     6301 ttgtcgatgt agcccagcag gcacggccgc ccgatctggc catggcgctt gaacaccccg
     6361 gcgctgcacg tgcgggccgg gaacacccgc aacagcaggt cgagggtctc ccggatcgcc
     6421 cacgcgtggg cgtacgggcc gaagtaccgc acgcccttgc gccgcggccc gcggtagacg
     6481 tgcagccgcg gaaactcctc gttcatcgtg accgcgagca ccgggtaggt cttgtcatcg
     6541 cggtaacgga cgttgaaccg agggtcgaac tccttgatcc agttgtactc gagctgcagc
     6601 gcctcgacct cggtggcgac gacggtccac tccaccctgg ccgccgtggt gaccatctgc
     6661 cgcgtgcgcg ggtgcaggcc ggcgaggtcg gcgaagtagg agttgaggcg ctgacgcagg
     6721 ctcttcgcct tgccgacgta gatcactcgg ccgtgctggt cggagaagcg gtacacccca
     6781 ggggcctccg ggatggaccc gggggccggg cggtaccgcg cgggatcaga cgaggcgggc
     6841 tgtggcatgg cccctccagg gtaggaccgc ccccgacacg ccgtgcctgc accgccccct
     6901 cggcgcgtcg cgggcgtggt gtaggtggaa ctgtcaccgc gccgtgatag gactccctcc
     6961 agtgcgaaca gctgttcgaa caaggggggg atcccatggg ggtcgacaag ctcgacacgg
     7021 tccgcaagct gctcgtgaag gcggagcgcg cagccacggg tgcggaggcc gagacctaca
     7081 cggcgaaggc gatggagctg atggcccgcc acggcatcga cgccgcgttg ctggccgccg
     7141 cccgccccga caccgaccgg gtcggcccgc agcgcatcga gatgcaggat ccctacagcg
     7201 cggggaaggc caggttgctg ggctggacgg ccagcgcgct cggctgccga tgggtgctgc
     7261 acggggcgtg gggcggcaag gtcgcggcgg tcacggtatt cggccacgca tccgaccgcg
     7321 aacgcgtgga gctgctctac acgtcgctgc aactgcaggc cagcacgcag ctggtccgtg
     7381 ttcgcccggc ctggcccggc gagtccgtgg ccgcctaccg gcgctcatgg ctgcacggct
     7441 tcgcggtcga ggtgcatcgg aggctcagca gcgccgaggc ccgggccgcc gcgagcgctg
     7501 ccgaacccgc agcagacggc cccggcgtcg cgctggtgct ggccgatcgc cgcgagcggg
     7561 tcgaccgcgc ctacgccgcg gccttcccgc ggctgggcac ggcccggcgc accgtcctgt
     7621 ccggctcggg gttcgctgca ggagccaggg caggcgagcg ggccgatctc ggtcgcgcca
     7681 ccggcatcga caggggccga cggcgggcgc tgggcgcctg acgtgaccgg acgcacgtca
     7741 cgccgacagc gcgatgagtc cgtccaccac ctggtcgagg agcccgccca ccacgtgggg
     7801 ccgggccacg acgtcgggca ccatgccctc aagccgggtc
//
LOCUS       DASRSC010000260         8549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2719289,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000260 DASRSC010000000
VERSION     DASRSC010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8549
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2719289"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..563)
                     /locus_tag="VFE39_12720"
     CDS             complement(75..563)
                     /locus_tag="VFE39_12720"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET6259507.1"
                     /translation="MKLLDRLLKPTVERLLAERIEAEGLDALYRYRVHSDPARLRIHP
                     TAVVNNAIFNTGGGYITVGAYVFFGHNVCVLAGVHEVDKFGLERQQAIPRSGYDIEIG
                     EGAWLATNVLVAGPVKIGTHAVVGGGSVVRKDVEPYAVVAGNPARFIRFLNRDDGGDP
                     HG"
     gene            complement(665..1261)
                     /locus_tag="VFE39_12725"
     CDS             complement(665..1261)
                     /locus_tag="VFE39_12725"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6259508.1"
                     /translation="MARVPAQERRRQLVDAAFRVIGREGFAAATTRRVCAEAGASLAA
                     FHYCFASKQELLVELTAQTMAGLVAARAGVHVGGSVTDSLRSTLRGYWETVEQDPNRE
                     SVLMALTQHALREPALAGIAEQQYAAYHRTARETLEEIAAGCGVRWALPVGQLARMLV
                     VVTDGVTLAWLVDRDNDAALAALDAFADQLAGLAVAMG"
     gene            1363..2808
                     /locus_tag="VFE39_12730"
     CDS             1363..2808
                     /locus_tag="VFE39_12730"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6259509.1"
                     /translation="MGEFGYASQFPRVLRRFALFAIAFSIVSITTGIFVNYAYGLIQL
                     GPAAIWLWPVAAAGQFLVVLVLAELAGYFPLAGANYQWTARLVGSRFGYVVGYLGVLY
                     GAVGLPGIALIGLAPLTATVLGLDAENVRLLLFIAVLALVVAYLVNIVRVQLAVRVNN
                     VAVFAEIIGTVVLSVVVFLAWVRGGADTPDGGHGFAFLFSTATQAPLADALVGGALIG
                     IFTLVGFEAAADMAEEAVDARRAVPRAMILSVVVSGVLGMLALVGFTVAIPDLPAIQA
                     SPVPLAAIAEYWLGPLVTKIFLAIVVFSMFALIVVAAASNSRLIFAMARDGMLPFSAA
                     LRRVHPRTGTPVPALVTSLVVCLVLLAYGTADGNAFAVLVGATAIVPYLVYLLTLGGY
                     LARRRALVGEGEGGFRLGAAALPVAGLALLWLVAVVAALTLPEAFRSADYVVLGALAL
                     AGLWYLAVLRRRLRDGTAGIGTARPDTVEIG"
     gene            2805..4115
                     /locus_tag="VFE39_12735"
     CDS             2805..4115
                     /locus_tag="VFE39_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HET6259510.1"
                     /translation="MSWRNWAGNQRTDPKRTVVARDVADVVEVVKGAQRDGLRVKAVG
                     SGHSFTAIAVPEDIALRPPANPNLLLVESVTGQVTVPAALPLHRLNLLLWERGLALPN
                     LGDIDAQTVAGAISTGTHGTGATHRGLAAQVRALEIVLADGSVMTCSATQHPEVFAAA
                     RIGLGALGVLTHVTLQAVPAFRLHAVETAMPLQEVLGALDELVAANEHFEFYWFPHTD
                     IAATKRNNRTEAAGPTRGRVAEWVGDDLLGNAAFELTCRLGAAVPRLVPPINRLMAGQ
                     MPAGEYVDRSYRVFTSPRRVRFVEMEYGVPREAIHEALAGLRAAAARHARAVTFPVEV
                     RFAAADDIPLSTAAGRDSAYIAVHVYRGQPHEAYFGAVEAVLAGVGGRPHWGKLHTQT
                     AATLCERYPAFDGFVALRDRLDPEGRFTNAYLDRVLGAPGTSVR"
     gene            complement(4190..4666)
                     /locus_tag="VFE39_12740"
     CDS             complement(4190..4666)
                     /locus_tag="VFE39_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6259511.1"
                     /translation="MTSPGVHLREITDHNRDAVCALRVRRDQKRFVASVASSLDDAAE
                     EPEANPWYRAVYSGDEPVGFVMLSWNVPPGRPGVIGRYFLWRLLIDKRHQRRGIGREV
                     LMQIIDLVRADGGTELFTSYEPGDGEPWPFYQQFGFQPTGEIDDDEIVLRLTLSRR"
     gene            4929..6530
                     /locus_tag="VFE39_12745"
     CDS             4929..6530
                     /locus_tag="VFE39_12745"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HET6259512.1"
                     /translation="MLDHDNEVVSAARRMSYGEQLARCARKFPHRVAFQHGPRSITFP
                     ELDERVTRLANALRERGVERGSRVAILMHNRIEVVEAYLAACRLGAMAVPLNFRLVPD
                     EIAYILGDCGAVALLVDEQLAGVAAKGMVGAEALGTVLATGGGEADVGPRAESYEDAL
                     ASASGTPPGIDVAENEPAFIMYTSGTTGRPKGAVLTHLNLVMAAFTGLAVSGGLGGTG
                     PDASLLGVPMFHIAGLSTSIRGLLDGGRVVIHGAANFDPVSVVDVLEREQITNCFFVP
                     SQWQAICAVPGVKDRRLALRNLVWGASPAPPSVLQALQDCFPGAPAYCAFGQTEMSPT
                     TTLLRGEDAVRKMGSVGTPLPSIEVRIVDEEMRDVPVGEVGEVVYRGPTMMLGYWNKP
                     EETAAAFAGGWFHSGDLCRMDDEGYLYVVDRKKDMIISGGENIYCAEVEAVIDCHPKV
                     AEVAVVGVAHPRWVQTPRAVVVPVDAADPPTEQEIIEHCRTRLASYKKPTSMVVTDAL
                     PRTASGKVQKFRLREQFGNTEVEAG"
     gene            6594..8180
                     /locus_tag="VFE39_12750"
     CDS             6594..8180
                     /locus_tag="VFE39_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6259513.1"
                     /translation="MQKAADLIEAYRVNGALGFWEIADAGPERVAIIDVDGSETTFGQ
                     LRDLANRISHGLRAQGLVDGDTVAVALPNHRAFLALQLATSQLGLYLTALNWHLTAPE
                     LAYILTDSETKLFVAGPRLAVAAAAAADEAGLSPQRRFGVPAFPGFRGLDELVDGMPA
                     TAPERRRQGSVMLYTSGTTGRPKGVRKPLRDATPELSFAARVSLLPRMLGRTAGSGVH
                     LVVAPLYHAAPNAYGSLSVQIGHTVALMDKWTPQLFLELVERHRVTASHMVPIMFHRL
                     LSLPADERARHDLSSLTRIVHAGAPCPVAVKQQMIDWLGPVLHEYYAATEGGGTYVGP
                     QDWLQHPGTVGRAWEDSEVVVLDDDGSALPAGEIGNIYFRSGDEFEYFKAPEKTAAAR
                     RGDLFTVGDLGHLDDEGWLYLSDRRSDLIISGGVNIYPAEIESALLTHPAVADVAVIG
                     MPDEEWGQRVAAMIEPRSFEDLAGESAERLAAELMEHCRGRLAGFKCPTSIEFRELPR
                     TSTGKLSRVRLRDDVLGVAR"
     gene            8356..>8549
                     /locus_tag="VFE39_12755"
     CDS             8356..>8549
                     /locus_tag="VFE39_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6259514.1"
                     /translation="MSTPAEGVLVVTIDRPQARNAVTAAVAQGIAEAMDRLDADDGLR
                     VGVLTGAGGTFCAGMDLKAFV"
BASE COUNT         1213 a         2957 c         3009 g         1370 t
ORIGIN      
        1 cagctactga tggacaccac ctcgaactga cgtgctgacg ggcgcgtcgg tgctcggggt
       61 gttcccgcca gagctcaacc gtgcgggtct ccaccatcgt cccggttcag gaacctgatg
      121 aacctcgccg ggttgcccgc caccaccgca tacggctcga cgtccttgcg caccaccgat
      181 ccgcctccga cgactgcatg cgtaccgatc ttgaccggcc cggcgaccag cacgttggtc
      241 gccagccatg cgccctcgcc gatctcgatg tcgtagccgc tgcgggggat ggcctgctgc
      301 cgctcgagac cgaacttgtc gacctcgtgc acgccggcga gcacgcagac gttgtgcccg
      361 aagaacacat aggcaccgac ggtgatgtag ccgccgccgg tattgaagat cgcattgttg
      421 acgaccgccg tcgggtggat tcgcagtcgg gccgggtcac tgtgcacgcg ataccggtag
      481 agcgcgtcca ggccttccgc ttcgatgcgc tccgcgagca gccgctccac ggtcggcttg
      541 agcaaccggt ccagcagctt catcggatac tccgctcgtt gcgtcccccg ccctgtgcgc
      601 gtcagccgga tgcccgcgaa atggcgtggc caaccgcagg ttcagacgtt cgcgccaggt
      661 gctgtcagcc catggcgacg gccagccctg cgagctggtc ggcgaaggcg tcgagcgcgg
      721 ccagcgcggc gtcattgtcc cggtcgacga gccaggccag cgtcaccccg tcggtcacca
      781 ccacgagcat ccgggcgagt tggcccaccg gcagtgccca gcgcaccccg cagcctgccg
      841 cgatctcctc gagagtctcc cgagccgtgc ggtggtaggc cgcatactgc tgctcggcga
      901 tgcccgcgag cgccggctcg cggagtgcat gctgggtgag cgccatgagc acggactcgc
      961 ggttggggtc ctgctcgacc gtctcccagt agccgcgcag cgtggacctc agcgagtccg
     1021 tgacgctgcc cccgacgtgc acgccggcgc gcgcggccac gaggccggcc atggtctgcg
     1081 ccgtcagctc cacgagcagc tcctgcttcg aggcgaagca gtagtggaac gccgcgagcg
     1141 atgcaccggc ctcggcacag acccgcctcg tcgttgccgc tgcgaagccc tcgcgcccga
     1201 tcacccggaa cgcggcgtcg accagctgcc ggcgacgctc ctgtgccggc acccgagcca
     1261 tgacaaccct ccgtccggac cgaccgggtc aactgtccca gacgattgac acactgtgac
     1321 tgccgtcgca ctctgttgcc cacataacca gtaggaggag cgatgggtga gttcggctac
     1381 gcatcgcagt tcccccgggt gctccgccgg ttcgcgctct tcgcgatcgc gttctcgatc
     1441 gtctccatca ccacggggat ctttgtcaac tacgcctacg gcctcatcca gctcggcccg
     1501 gccgcgatct ggctctggcc ggttgccgcc gcaggacagt tcctggtggt actcgtcctc
     1561 gccgagctgg ctgggtactt cccgctcgcg ggcgccaact atcagtggac ggcccggctc
     1621 gtcggatccc gcttcggcta cgtcgtggga tacctcggcg tgctctacgg cgcggtcggg
     1681 ctgcccggga tcgcgctgat cggactcgcc ccactgacgg ctacggtcct cgggctggac
     1741 gcggagaacg tccggctcct gctgttcatc gcggtgttgg cgctggtggt cgcctacctc
     1801 gtcaacatcg tgcgggtgca gctggccgtg cgggtcaaca atgtcgcggt gttcgccgag
     1861 atcatcggca ccgtggtgct cagcgtcgtg gtgttcctgg cgtgggtgcg cggcggcgcg
     1921 gacacgcccg acggcggaca cgggttcgcg ttcctgttca gcaccgccac ccaggcgccg
     1981 ctggccgacg cgctcgtcgg tggtgcgctg atcgggatct tcaccctcgt cggcttcgag
     2041 gcggcggccg acatggccga ggaggccgtc gacgcccggc gggccgtgcc gcgggcgatg
     2101 atcctctccg tcgtggtctc cggcgtgctg gggatgctcg cactggtcgg tttcactgtg
     2161 gcgatcccgg acctgcccgc gatccaggcg tcgccggtgc cgttggccgc gatcgccgag
     2221 tactggctcg gtccactcgt gacgaagatc ttcctcgcga tcgtcgtgtt ctcgatgttc
     2281 gcactcatcg tcgttgcggc cgcatccaac tcccggctga tcttcgcgat ggcgcgcgac
     2341 gggatgctgc ccttctctgc agccctgcgg cgcgttcacc ctcgtaccgg cacgcccgtt
     2401 cccgcgctgg tcacgtcgct ggtcgtctgc ctggtgctgc tggcctacgg gacggcggac
     2461 ggcaacgctt tcgcggtcct ggtgggggcg acggcgatcg tgccgtacct ggtctacctg
     2521 ctcaccctgg gcgggtacct cgcccggcgg cgggcgctgg tcggcgaggg cgagggcggc
     2581 ttccggctcg gggccgccgc actgcccgtc gcagggctgg cgctgctctg gctcgtcgcg
     2641 gtcgtggcgg cgctgacgct gcccgaggcg ttccgctcgg ccgactatgt cgtgctcggc
     2701 gcgctggccc tggccggact ctggtatctc gccgtgctgc ggcgccggct ccgcgacggc
     2761 acagccggga tcggaacggc gcgccccgac accgtggaga tcggatgagc tggcggaact
     2821 gggcgggcaa ccagcgcacg gacccgaagc ggacggtcgt cgcgcgggac gtcgccgacg
     2881 tcgtcgaggt ggtgaaggga gcgcagcgcg acggcctacg ggtcaaggcg gtcgggtcgg
     2941 ggcactcgtt caccgccatc gccgtgcccg aggacatcgc gctgcgccca cccgccaacc
     3001 cgaacctgct gctcgtcgag agcgtgacgg ggcaggtcac cgtccccgcc gcgctgccgc
     3061 tgcaccggct caacctgctg ctgtgggagc ggggcctcgc cctgcccaac ctcggtgaca
     3121 tcgacgcgca gaccgtggca ggcgcgatct ccaccggaac ccacggcacc ggcgccacgc
     3181 accgcgggct cgccgcccag gtacgggcgc tggagatcgt gctggccgac gggtcggtca
     3241 tgacatgttc ggccacgcag caccccgagg tcttcgcggc cgcccggatc gggctgggcg
     3301 cactgggagt gctcacgcac gtgacgctgc aggccgtccc ggcgtttcgg ctgcacgcgg
     3361 tcgagaccgc gatgccgctt caggaggtgc tcggcgcgct cgacgagctg gtcgcagcga
     3421 acgagcattt cgagttctac tggttcccgc acaccgacat cgccgccacc aagcgcaaca
     3481 accggaccga ggcagccgga ccgacccgcg ggcgcgtcgc cgagtgggtg ggcgacgacc
     3541 tgctcggcaa cgcggcgttc gagctcacct gccggctcgg cgctgcggtg cccaggctcg
     3601 tgccgccgat caaccggctg atggccgggc agatgcccgc aggcgagtac gtcgaccgtt
     3661 cctaccgggt gttcaccagc ccgcgccgcg tccggttcgt ggagatggag tacggcgtcc
     3721 cacgggaggc gatccacgag gccctggccg ggctgcgcgc cgccgccgcc cggcacgcgc
     3781 gcgccgtcac gttcccggtg gaggtgcgct tcgccgccgc cgacgacata ccgctctcga
     3841 cggcggccgg gcgcgacagc gcatatatcg ccgtgcacgt gtaccggggc cagccccacg
     3901 aggcgtactt cggcgccgtc gaggcggtgc tggcgggcgt gggcgggcgc ccgcactggg
     3961 gcaagctgca cacacagacc gcggcgacgt tgtgcgagcg ctacccggcg ttcgacggct
     4021 tcgtggcgct gcgcgaccgg ctcgacccgg agggccggtt caccaacgcc tacctcgacc
     4081 gcgtgctggg ggcaccgggg acctccgtga ggtgacaacg ccctgagcgc gaacccgcgg
     4141 ggaatgatct gatcggcgcc gatggtgctc ggtggagtcc tctgtgcgct caccgcctcg
     4201 acagcgtcag tcgcaggacg atctcgtcgt catcgatctc accggtgggc tggaaaccga
     4261 actgctggta gaagggccac ggttcgccgt cgccgggctc gtagctggtg aacagctccg
     4321 tgccgccgtc cgctcgaacc aggtcgatga tctgcatcag cacctctcgg ccgatcccgc
     4381 gccgctggtg gcgcttgtcg atgagcagcc gccacaggaa gtaccgcccg atgactccgg
     4441 gacgacccgg cggcacgttc cacgagagca tcacgaagcc gaccggctcg tcgcctgagt
     4501 acacggctcg ataccaggga ttggcctcag gctcttcggc tgcgtcgtcg agcgagctgg
     4561 ccaccgaggc cacgaacctc ttttggtctc gtcggacgcg cagtgcgcag accgcgtcgc
     4621 ggttgtggtc ggtgatctcc cgtaggtgca cgcctggaga cgtcatgcca cgcccttcct
     4681 gtgtccagca gcggacctcc ggtccgggcc tcgggtggca gagcagcgta gcccggtcac
     4741 gagttcggcc cgacatgtca cggtcatcgg tcgggcggcg acggtccttg acagcgccgg
     4801 gtggtcgtga tgcgatcgga tcgacttaac gatcgatcag ttatcggccg aaccactctg
     4861 tcgacggccc cgtcctgcgc agctcgatca gccattcgtt gccgagtgga gcgagaagga
     4921 ggagccgcat gctggatcac gacaacgagg tcgtgtccgc tgctcgtcgc atgtcctacg
     4981 gcgaacagct cgcccggtgt gccaggaagt tcccgcaccg ggtggccttc cagcacgggc
     5041 cccgctcgat caccttccct gagctggacg agcgggtcac ccggttggcc aacgcgctgc
     5101 gcgagcgcgg cgtcgaacgg gggagccggg tcgcgatcct gatgcacaac cggatcgagg
     5161 tcgtggaggc ctacctggcc gcctgccggc tcggtgcgat ggccgtgccg ctgaactttc
     5221 ggctggtccc cgatgagatc gcctacatcc tcggcgactg cggggctgtt gcgctgctgg
     5281 ttgacgagca gctcgccggc gtcgcggcga agggcatggt gggcgccgag gcactgggca
     5341 ccgtgctggc cacgggcgga ggagaggccg acgtcgggcc gcgcgcggag tcctatgagg
     5401 acgcgctcgc ttccgcttcg ggcacgccac ccggcatcga cgtggccgag aacgagcccg
     5461 ctttcatcat gtacacctcg ggcacgaccg gcaggccgaa gggcgccgtc ctcacccacc
     5521 tgaacctggt gatggcggcg ttcaccggcc tcgcggtctc cggtgggttg gggggcacgg
     5581 gcccggacgc gtcactgctg ggcgtgccga tgttccacat cgccgggttg agcacctcga
     5641 ttcggggcct gctcgacggc ggacgtgtgg tgatccacgg cgctgcgaac ttcgacccgg
     5701 tgagtgtcgt cgacgtgctg gaacgcgagc agatcaccaa ctgcttcttc gtgccgagcc
     5761 aatggcaggc catctgtgcc gtgccggggg tgaaggaccg tcggctggcg ctgcgcaacc
     5821 tcgtctgggg cgcctcgcct gcgcccccgt cagtgctgca ggcgctgcag gactgttttc
     5881 cgggcgcccc cgcctattgc gccttcgggc agaccgagat gagccccacc accaccctgc
     5941 tgcgcgggga ggatgcggtg cggaagatgg gctcggtggg caccccgctg ccgagcatcg
     6001 aggtgcgcat cgttgacgag gagatgcgtg acgtccccgt cggtgaggtc ggcgaggtgg
     6061 tctaccgggg cccgacgatg atgctcgggt actggaacaa gcccgaggag accgccgccg
     6121 cgttcgccgg gggctggttc cactccgggg acctgtgccg gatggacgac gagggctatc
     6181 tctacgtcgt cgaccgcaag aaggacatga tcatctcggg tggagagaac atctactgcg
     6241 cggaggtgga ggccgtgatc gactgccacc ccaaggtcgc cgaggtggcg gtggtgggcg
     6301 tcgcccaccc gaggtgggtg cagacgccgc gcgccgtcgt cgtgcccgtc gacgcggcgg
     6361 accctccgac ggagcaggag atcatcgagc actgccgtac ccggctcgcc tcgtacaaga
     6421 agccgacctc catggtggtc accgacgctt tgccccggac cgccagcggc aaggtgcaga
     6481 agttccggct gcgcgagcag ttcgggaaca cggaggtgga ggcggggtga ggcgagcgcg
     6541 ttgagctgcg ggtgaccgat cttcaccggt gtgagacgag gggagctgca gcaatgcaga
     6601 aggcggcgga cctcatcgag gcctaccggg tgaacggggc cctcgggttc tgggagatcg
     6661 ccgacgccgg tcccgagcgg gtcgcgatca tcgacgtcga cgggtcggag accacgttcg
     6721 gacagctgcg ggacctggcc aatcggattt cgcacgggct gcgcgcgcag gggctcgtcg
     6781 acggcgacac cgtggccgtc gcactgccca accaccgggc cttcctcgcg ctgcaactgg
     6841 ccacgagcca gctcggcctc tacctgacgg cgctcaactg gcacctgaca gcaccggagc
     6901 tcgcctacat cctgaccgac agcgagacga agctgttcgt cgccgggccg cggctggccg
     6961 tcgccgcggc cgccgcggcc gacgaggcgg gtctttcacc acagcgccgg ttcggggttc
     7021 ccgcgttccc cggcttccga ggactggacg agctcgtcga cggcatgcct gccaccgcgc
     7081 ccgagaggcg gcggcagggg tcggtgatgc tatacacgtc cgggacgacc ggccgtccca
     7141 agggtgtgcg caagccgctc cgtgacgcca ctcccgaact gagcttcgcg gccagggtgt
     7201 cattgcttcc ccggatgctc ggtcgcactg cgggatccgg cgtgcatctg gtggtggcac
     7261 cgctctacca cgcagcaccg aacgcgtacg gcagcctgtc ggtgcagatc gggcacaccg
     7321 tggcgctgat ggacaaatgg accccgcagc tgttcctcga gctggtggag cggcatcggg
     7381 tcaccgcatc gcacatggtg ccgatcatgt tccaccgact gctgtcgctg cccgccgacg
     7441 aacgggctcg ccacgatctc tcgtccctga cccgcatcgt gcacgccgga gccccgtgcc
     7501 cggtggccgt caagcaacag atgatcgact ggctgggccc ggtgctgcac gagtactacg
     7561 cggccaccga gggtggtggc acctacgtcg gcccccagga ctggctgcag catcccggca
     7621 cggtcgggcg cgcctgggag gacagtgagg tggtggtcct cgacgacgac ggcagcgcgc
     7681 tgccggcagg cgagataggg aacatctact tccgcagcgg ggacgagttc gagtacttca
     7741 aggcgccgga gaagaccgcc gccgcccgcc gcggcgacct gttcaccgtg ggcgacctgg
     7801 gccacctcga cgacgagggc tggctctacc tgagcgaccg gcggtcggac ctgatcatct
     7861 ccggcggggt gaacatctat cctgccgaga tcgagtcggc gctgctcacc caccctgcgg
     7921 tcgcggatgt cgcggtgatc ggaatgccgg acgaggagtg ggggcagcgg gtggcggcga
     7981 tgatcgagcc gcgctcgttc gaggacctcg caggggagtc cgcagagagg ctggctgccg
     8041 agctgatgga gcactgccgg ggtcggctcg ccggcttcaa gtgcccgacc tccatcgagt
     8101 tccgggagct gccccggacc tccactggaa agctcagccg cgtcaggctc cgtgacgacg
     8161 tgctgggtgt cgcccgatga gcgagcttgc gagtgaatca gtgtcactgt gccgtcgcgc
     8221 agcgccgacc gagcgtagcg agggagtgcg atgagcgggt gcccggcccg cccgattctg
     8281 gtgctgtgcg ccttcctcga ggagcgccgc gccgggttca ccgaccgata gggagtgtcg
     8341 ttgtcagagg tactcatgag cacgcccgcc gagggcgtgc tcgtcgtgac catcgaccgg
     8401 ccgcaggcgc gcaacgccgt cacagcagcc gtcgcacagg ggatcgccga ggcgatggac
     8461 cggctcgacg ctgacgacgg cctccgcgtc ggggtgctca ccggcgcggg gggcacgttc
     8521 tgcgccggca tggacctcaa ggccttcgt
//
LOCUS       DASRSC010000261        20990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5669029,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000261 DASRSC010000000
VERSION     DASRSC010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 20990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20990
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5669029"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VFE39_12760"
     CDS             <1..420
                     /locus_tag="VFE39_12760"
                     /inference="COORDINATES: protein motif:HMM:NF040114.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6474 family protein"
                     /protein_id="HET6259515.1"
                     /translation="ASRAKRLIGVSKVVAPLLAPYALAAAGVLRARWDAYRAARLGVD
                     PSQLSAYAGPGGALHARLSRVAEALNELESRAGAGAADAARTFAVETRPRLVDLAVAV
                     RAAEQMPTARRRTAYRAIGGELGRIETDLLTHLGIST"
     gene            446..982
                     /locus_tag="VFE39_12765"
     CDS             446..982
                     /locus_tag="VFE39_12765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259516.1"
                     /translation="MSGGSAGGRRAVRGAALAVLSTLLTAVGHVAGGGAVPDLAVLVV
                     LFPLLAVVFVTVAERCRSLARMVTTLAAGQLALHLLMDLLRPAHDLMEPVVASGTGML
                     GMHAAVTLVLAVVLRHADGAAVGLVDAVRRVLPRRLTPLPADRPLPTRAVPGPAVSAR
                     LARALAVAHARRGPPVGC"
     gene            1018..1755
                     /locus_tag="VFE39_12770"
     CDS             1018..1755
                     /locus_tag="VFE39_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009085669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcnI family protein"
                     /protein_id="HET6259517.1"
                     /translation="MSSSVRRSALRAGTVTVVAAAVALVGAAPALAHVTAQPGRAEQG
                     GYTVIAFRVPTESDTAGTVKLEVKLPVDHPITSVRTTPRPGWTATMTKTGLNPPIQSS
                     GRTITETVSAVTWTADPGTRIGPGEFAEFPLSLGPLPTDTNQLVLPAVQTYDDGEVVA
                     WDQPPATDGSEPERPAPTVALSPAAAGDADVATSTTGGTTTTAAANGTDTTARWLGGG
                     GLLVGALGLGVGVGAVLRNRRAAGAGR"
     gene            1752..2324
                     /locus_tag="VFE39_12775"
     CDS             1752..2324
                     /locus_tag="VFE39_12775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HET6259518.1"
                     /translation="MTARVLRAAAVTVLCGLALLLGAGQALAHTRLESSDPADGASLA
                     AGPQRVSLTFNESMQPGFTTLTVVGPDQARYQTGDVTANGGTVSIGVRPLGPAGRYEI
                     GYRVVSDDGHPVTGSVAFTLTTPGPGSTAAAASPTATGAAAAPAPSTPVAAEAPATQD
                     GGGAPVWPWIVGAVVLVGGGVVAAMRLGRG"
     gene            2321..3316
                     /locus_tag="VFE39_12780"
     CDS             2321..3316
                     /locus_tag="VFE39_12780"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HET6259519.1"
                     /translation="MTAPGGGPRPVDRYLLPVAWAGLTVGASFLASVLTGGLAGSTLP
                     VLVGITVTRSGMDIAGVACVGLTLLGALLPQARALPGRALRDLIRVRTTADRSLVLLA
                     ATWLALVLVGILFRVASAFGRPVAELTGGEVVEWATELAAGRGTVLTAGCAAVVLGCA
                     VARLHDRNLVQVRIPMVAALLGLITPGVTGHTGSASDHDLAVISVGAHAGAAALWVGG
                     LGAVLVLVARHRPLLDAVLPRFSRLAGLCVVTITLTGLLSAQFGLGSWAALVSTGYGR
                     LVLAKAGCLVVLACLGGLARRRLAAGRTPVLRWAGLETALMAVTLGLAAALSQAA"
     gene            complement(3330..4760)
                     /locus_tag="VFE39_12785"
     CDS             complement(3330..4760)
                     /locus_tag="VFE39_12785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6259520.1"
                     /translation="MTVQGERAATTFDVVVVGGGAVGENAADYATRAGLSAVIVEAEL
                     LGGECSYWACMPSKALLRTGHAVAAVRRLPGTTADFDPTAVLARRDSFTNNWNDENQV
                     EWAVGAGITLVRGHGRLTGEKTVEVVGGPAGAQTLTARRAVVLCTGSVPVRPPVPGLD
                     TVRTWTSREATSAKEIPARLGVLGGGVVGCELAQAFRRLGSEVTLVQNEPRLLPAMEP
                     FAGERVAAALREDGIDLWLDQGLDSAAPDGDPITMHVGSATITVDELLVATGRRPNTD
                     SVGLETVGLEPGKPLTVDDTGLVEGVEGEWLYAAGDVTGRALLTHQGKYAARIVGAVI
                     AARSIGAELDTRPWGEHVATADHAAVPQVVFTDPEVASVGRTEAQARAAGIDVRVVDL
                     PIAVGGSSLHADGYDGAARLVVDEGRGILAGVTFVGQDVAEMIHAATVAIVGEVPMGR
                     LWHAVPAYPTISEVWLRLLEAYREPA"
     gene            complement(4757..5416)
                     /locus_tag="VFE39_12790"
     CDS             complement(4757..5416)
                     /locus_tag="VFE39_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding oxidoreductase"
                     /protein_id="HET6259521.1"
                     /translation="MQVVIAGAHGKIGLRLAALLAGRGDVVTGVVRNPDHRADLERAG
                     ASAAVVDLECASADELAAHLTGADAVVFAAGAGPGSGVARKDSVDRAAAELLATAAQL
                     AGVRRYLLVSSPGVDAPPEPGTDEVWAAYLRAKKAAEEALRATDLDWTILRPGRLTDD
                     PGTGRVLLAPPPVERSDVTRDDTAAVLAALLDAPRTARRVLELREGDHDVHEAVAAVS
                     G"
     gene            complement(5525..5977)
                     /locus_tag="VFE39_12795"
     CDS             complement(5525..5977)
                     /locus_tag="VFE39_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HET6259522.1"
                     /translation="MTTDAGWVGTAMGQAVPWVRTTGVEFGEVTPERAVAYLPDAAEM
                     HNHVGGPHAAMIFGLGETASGAVAMAAFGASMDRATPLVVRSEIQYVRLARGTLTAAA
                     VLDRPAADVLAELNAGTRPEFTVTVVITDAGGGETTRMSVVWTLRPNR"
     gene            6225..7253
                     /locus_tag="VFE39_12800"
     CDS             6225..7253
                     /locus_tag="VFE39_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HET6259523.1"
                     /translation="MKAVRVHEYHVDPTIDDIPEPKITGPLDVIVKIGGAGVCRTDLH
                     ILEGQWADAMNPQLPYVIGHENAGWVHEVGEGVTNVAVGDTVILHPQPSCGLCLACRA
                     GRDMQCENGFFPGLSDNDGGMAEYLRTTARACVKLNPDTNPADVAALADAGITAYHAV
                     RKAVPSLYPGTTAVVQGAGGLGHIGIQALAALTATRIIAVDRNPEALALAKQIGADET
                     VLADGSHIDAVKDLTGGNGANVVFDFVAEQGAENDAWQMTAPAGSDYIIGYGGELRIP
                     TLDFVGGEKNVIGNIVGTYNDLAELMVLAQAGKVTLHTRQYPLDAALDALHDLDAGRV
                     RGRAILVP"
     gene            7299..8363
                     /locus_tag="VFE39_12805"
     CDS             7299..8363
                     /locus_tag="VFE39_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6259524.1"
                     /translation="MYEKDGEKYFIVDSHSHFWDASRENWKPGAEEYAKGWIDCFYGY
                     HQLGPPETHWDYEKYLKVTPEDFERDMFIEGHVDHAIFQSTYLKEWYTTGFNTAAKNA
                     ELLERWGDRLIVNGRFDPRDGDAGMAELEADAEKYGLKGVKLYTAEWNGGSRGWKLTD
                     PESYRFLEKCQELGIKNIHVHKGPTIWPLNKDAFDVADVDQTATDFTDLNFIVEHVGL
                     PRIEDFCFMAVQEPNVYAGLSVVIGGLMHARPKFFAKVIGELMFWVGEDKMLFGGDYN
                     IWVPKWQVEGFVDWQMPDDEAFTDYAKLTTDTKKKILGLNAARLYDIPVPAELQLSTT
                     AGADLQPGEELVKDVAGAQA"
     gene            8360..9082
                     /locus_tag="VFE39_12810"
     CDS             8360..9082
                     /locus_tag="VFE39_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HET6259525.1"
                     /translation="MTPRPPGSDLRDAVWAALGTVLDPELDEPITDLDFVESCTVSEH
                     DTADAPGVVATVVLRLPTFFCAPNFSFLMVADAFDAVSTVPGVRRADVTLADHHASAE
                     INGGVAAQAGFVGTFGSEAAAELDELRAQFLRKAALAGQDRVARPLVDAGTGPDELAE
                     LTLGSVPPSPDLSRLRERRAALGMAHGDEAPLLVHADGSRVTAAQVPLHLRRARLQRI
                     GIETNGEYCKSLLQTRYPTAVR"
     gene            9153..9848
                     /locus_tag="VFE39_12815"
     CDS             9153..9848
                     /locus_tag="VFE39_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HET6259526.1"
                     /translation="MDVRALVFDVFGTVVDWRSGVAREIARLLGERVDADALADAWRG
                     RYLPSMDRVRRGELPWTSLDGLHRASLDELLGEFGAPETPEQVRDELVLAWHRLDAWP
                     DSVEGLTLLKRHHVVASLSNGNVALLVNMAKHAGLPWDTVLSAELFGHYKPDAEVYDG
                     AARLLGLRPEQVLMVAAHTDDLAAARSRGLRTAYVHRPLERGPDAPPPPPTDPDADLT
                     VGSMTELAHRLTS"
     gene            complement(9838..10578)
                     /locus_tag="VFE39_12820"
     CDS             complement(9838..10578)
                     /locus_tag="VFE39_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6259527.1"
                     /translation="MSDEGPRGWGAPLHARALDAAGVGPGCTLLDLGCGAGAFARAAA
                     DRSAVVTGVDVDRSAVVAAAAAVPEGTFRVGDAHDPPAGPFDVVAAVQLLSHVTNPLA
                     VLRSAGRVGSTVVLTVWGREDECDVRAFGEALAPWLPPRRAPTGPPPLTEPARLRKLV
                     TLAGLTVDVFDEVACPFDYPDADALLGPLLMSGIGRQAVGRAGEPAVRRAVLERLSPQ
                     RTADGGYRLVNTFRVLATVAQEGRGVRR"
     gene            complement(10610..11728)
                     /locus_tag="VFE39_12825"
     CDS             complement(10610..11728)
                     /locus_tag="VFE39_12825"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET6259528.1"
                     /translation="MHGRTEAPFRADHVGSLLRPQNLLAAREQFAAGNLDADGLRAAE
                     DTAIRDAVAMQEEIGLQAATDGEFRRTSWHMDFIYQLGGVSKTDEQIRVRMRNAEGEG
                     AFTSAGLAIHDKVRLERPIFADHYRFLASITTTAVPKLTIPSPSMVHYRGGTAALDKG
                     VYPDVEEFWADLSAAYADEVRALAGLGCRYLQLDDTSLAYLNDPAQRAEMAAKGEDAD
                     HAHLRYIRQINAAIADRPADMKITTHMCRGNFRSSWAAEGGYDFVAEAVFGDLEVDGY
                     FCEFDDERSGGFAPLRFVPPGKQVVLGLVTTKRGELEDADALKRRIEEASKYVPLDQL
                     CLSPQCGFSSTVEGNSLTQEEQVAKLRLIVKVAQDVWG"
     gene            11821..12654
                     /locus_tag="VFE39_12830"
     CDS             11821..12654
                     /locus_tag="VFE39_12830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HET6259529.1"
                     /translation="MQHGIVTFLTDYGIDGPTLATAVEERGLDALFLTEHTHIPTSLK
                     TRWPGGEDLPRHYSHTFDPFVALAAMAAVTDRIALGTAVSLVLQHHPIGLAKTVASVD
                     HLADGRFELGVGPGWNREEMANHGVDPRTRTARMLEHIAAMRAIWENDEAEFHGTFVD
                     FDPIWSWPKPVRMPPVLIGGEGPTVLDRVLSHGDAWLPRRFAPEEIDVLAARIAELRS
                     RAADAGRGHIPVTMYGGDPDRKALDSYAGAGVDRVLFSLPAAGPETVLPGLDEIADLV
                     A"
     gene            complement(12671..13456)
                     /locus_tag="VFE39_12835"
     CDS             complement(12671..13456)
                     /locus_tag="VFE39_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET6259530.1"
                     /translation="MAPARTTGSSITRALRVVEAVAAAGDGVTAKAIARRLGAPLPTV
                     YRALGTLVEEGYLVRLHDVRGYGLGYRVAELHRGLTDQVRPPASVRAILHEVHTAAEA
                     AAYLAVLRDVDIVVAYVDDCTDHPRPDGMRVGEPTAPHATAAGKVMLADLRPGRLAEL
                     IARTGMPRLAPRTVADRRALDRELMRVRSDGAAVEVEEYQPGVAGIAAPVRGPGGDVT
                     GALGISVTRAAFAARRWDLERVVRQAAARVGETAHRASAGWPC"
     gene            13519..13761
                     /locus_tag="VFE39_12840"
     CDS             13519..13761
                     /locus_tag="VFE39_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HET6259531.1"
                     /translation="MNDDELHRQIDDLVAEEHRLERAHVGQPLSESEQQRMEQLGVQL
                     DRYWDLLRQRDARRRAGLDPDDAQERSGDVVEGYRQ"
     gene            13841..14491
                     /locus_tag="VFE39_12845"
     CDS             13841..14491
                     /locus_tag="VFE39_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET6259532.1"
                     /translation="MQITPVSPDLLVEEVTGLVAARPGRVRLALDGPPPARPVALAER
                     VAVELRALGRAVLVVAAADFLRPASVRLEYGREDPDEFLDGWLDVGGLRREVLDPAGP
                     DGSGRVLPRLWDAAADRAYRDGYTELPADGVVLLAGALLLGRGLPLDMSVHLRMNPAA
                     LARTLPDDERWTLPAYARYDAEHDPARAADLLVLVDHPDRPAIRRPERGDCGRGGS"
     gene            complement(14473..14555)
                     /locus_tag="VFE39_12850"
     tRNA            complement(14473..14555)
                     /locus_tag="VFE39_12850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14520..14522),aa:Leu,seq:cag)
     gene            14688..15962
                     /locus_tag="VFE39_12855"
     CDS             14688..15962
                     /locus_tag="VFE39_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 and FHA domain-containing protein"
                     /protein_id="HET6259533.1"
                     /translation="MGRVDRFERRLQGLVGDAFARVFGGSVVPQEVVQALLREAEDHI
                     EELAGGRLLAPNRFTVLLCPSDLDRMAGDRTEIVNTLSASVTEQLADQGWDTYGEVVV
                     FLERSDALHTGQFRTRSSVDPDASRRDAIRARDAGEAPMSQQPGHPEENGQYGYDRGA
                     PGGYGQQTYAPPAYDQRQGGYDQGEQPPQQPGYGQQPGYPQQGGGGGYPQQGGYDQGY
                     GQQQGGGYPPQQGGGYGGQQGGGYEQGYGQPGQPGQPGQPGYPQQGGGYEQGYGQPGY
                     GQPGYGQPGYAPGYDAGYQQGGYQQPYEQGYDAGYDQQQQGYGQQGGYGGYQQGLSAS
                     LSLDDGSGRTYDLTQGSHVIGRGQDSSFRLPDTGVSRRHLEISWDGHTATLTDLGSTN
                     GTTVNGNPVQTWQLGDGDVIRVGHSSLVFRTQ"
     gene            16019..16474
                     /locus_tag="VFE39_12860"
     CDS             16019..16474
                     /locus_tag="VFE39_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010313255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HET6259534.1"
                     /translation="MPELVLQLTRAGFLALLWLFVLVTLRVVRSDLYAASGLRALVPG
                     GRTATRTGRGRTARQLLVTQGALAGTRVSLDSRPILIGRADDSTLVLDDDYASTRHAR
                     IALQGEEWYVEDLGSTNGTYLDRAKVTGPTRVPLGVPVRIGKTVIELRS"
     gene            16566..17903
                     /locus_tag="VFE39_12865"
     CDS             16566..17903
                     /locus_tag="VFE39_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HET6259535.1"
                     /translation="MTLVLRYSARSDRGLVRQNNQDAVYAGPRLLALADGMGGHAAGE
                     VASSLVISAFAPLDEDDPGDDLLAELRHATVEGNAAITRHVADAPDLDGMGTTLTAIL
                     FAGSRLGLVHIGDSRAYQLREGAFTQITKDDTFVQSLIDEGRITEEEAHTHPQRSLLL
                     RAITGQDVDPSLTIREARAGDRYLLCSDGLSGVVSDETLADTLQAYRDPRECADRMIE
                     LALRGGGPDNITCIVADVVDIEFGEDAPIIGGSVGDGSEDGPRPDSAAARASATTLTR
                     VAPQRVVAATKAPAARRSRVKIVMGVVTVLAVLIGGVVLARMWVLQQYYVGVDAQLVS
                     IFQGVHGEVLGIPLQRVAERTDITLDDLPETDRSAVRDGIRALDGLPGARGVVERLRG
                     RMLEPCPPPAPPQPPPPVPDPNLPPVPGAAPPADTTPLPTPAPEPGTTCRSVS"
     gene            17903..19342
                     /locus_tag="VFE39_12870"
     CDS             17903..19342
                     /locus_tag="VFE39_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET6259536.1"
                     /translation="MAAPDTRSEPAPLAPAPLPNGRGIELVLLAFAAVLVTGALVLVE
                     ANQEQQLTRSLLFVGLAYLGLFTIAHFAVRRFAPFADPLILPCVALLNGLGLVMIHRL
                     DLAEVDQALALGRDVPTELIGRQVAWTAIGLALFVAALWLVRDHRTLARYAYTAGFAG
                     IVLLALPGLLPSSISEVNGAKLWIRVGPVGIQPGEFAKILLIVFFAAFLVQKRDLFTT
                     AGRRFLGMELPRARDLAPLLLAWGMSVGVLVLENDLGTSLLFFGILLAMLYVATERVS
                     WMLIGLMFFFAGCTIAYHLFAHVRVRVQVWLDPFGDFANTGYQIGQALFSLGTGGVGG
                     TGLGAGRPDLVPFAESDFMLSSLGEELGLIGLAAILVIYLVLITRGLRSALAVRDSFG
                     KLLAAGLAFALALQIFIVAGGASKLIPLTGLTLPFLSYGGSSLVANYALVALLLRISH
                     AARAPLPRRPATPLPPIAEAGTELVERPR"
     gene            19339..20811
                     /locus_tag="VFE39_12875"
     CDS             19339..20811
                     /locus_tag="VFE39_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HET6259537.1"
                     /translation="MNTPIRRIAIAVMVMILLLMSNLTYVQVVKAGDYRNDPRNQRVL
                     LSEYSRKRGQISANNQILASSVETNDRLRYQRQYSDGAVYGPITGYYSVIYGSSGIER
                     ASDSVLNGSDDRLFGRRLSDLITGRDPSGGNVVLTIDPNVQQTAYDQLQRNGYTGSVV
                     AIRPQTGEILAMVSTPSFDPNPLASHNSDQQNSAWKADRDANPPVLINRAISETYPPG
                     STFKLIDTAAALSSGRYTPDSQLTAAPRVTLQGTNTTLENFNGNACGTSPTASLRDAL
                     QRSCNTAFATLGADLGEQAIRDQANAFGIGQSLSIPMPVAPSTIGDIPDTAALEQSAI
                     GQRDVALTPLQNAMIVATIANGGQVMAPHLVKEIQSQELDPVATTEPDRLGRAIDPTV
                     ARTLTDLMVNNENSYQGAGKITGVQIAAKTGTAEHGNDPKNTPPHVWYVGFAPANNPQ
                     VAVAVLVENGGDRNNLAATGGAVAGPIGRAVIRAALGGGQ"
BASE COUNT         2999 a         7706 c         7379 g         2906 t
ORIGIN      
        1 gcctcgcgag cgaagcgcct catcggcgtc agcaaggtcg tcgctccgct gctggcgccc
       61 tacgcccttg cggccgccgg ggtgctccgc gcccgctggg acgcctaccg ggcggcccga
      121 ctgggcgtgg acccgagtca gctgtccgcc tacgcggggc cgggcggggc cctgcacgcc
      181 cggctgtcgc gggtggcgga ggcgctcaac gagctggagt cgcgggccgg ggcgggcgca
      241 gccgacgcgg cccgcacgtt cgccgtcgag acacgcccgc ggctggtcga cctggcagtc
      301 gccgtgcgtg ccgccgagca gatgcccacc gcgcgccggc gcaccgcata ccgggccatc
      361 ggaggagagc tcggccgcat cgagaccgac ctcctcacgc acctgggcat cagcacgtag
      421 catggtccgg ctaccgtccg tccccatgag cggcggttct gcgggcggcc gccgcgcggt
      481 gcgcggtgcg gcgctggccg tgctgagcac gctgctgacc gccgtcgggc atgtcgccgg
      541 cggcggcgcc gtgcctgatc tcgcggtgct cgtggtgctg ttcccgctgc tcgcggtcgt
      601 gttcgtgacg gtggcggagc gctgccgcag cttggcacgc atggtcacga cgctggcggc
      661 agggcagctc gccctgcacc tgctgatgga cctgctgcgt ccggcgcacg atctgatgga
      721 gcccgtggtg gcgtcgggca ccggaatgct gggaatgcac gcggcggtca ccctcgtcct
      781 cgccgtcgta ctgcggcacg ccgacggagc cgcggtcggg ctcgtcgacg cagtgcgcag
      841 ggtgctgccc cggcgactga ccccgctccc ggccgaccgg ccactgccca cccgtgcggt
      901 acccggtccc gccgtgtccg cgcggctcgc gcgcgctctc gccgtcgccc acgcaaggcg
      961 cggccctccg gtggggtgct gaccagccca ctcacctagc ccacaccgga gacacccatg
     1021 tcctcgtccg tccgccggtc cgcgctgcgc gccggcaccg tcaccgtggt cgccgcggcc
     1081 gtcgccctcg tcggcgcggc gcccgcgctc gcccacgtca ccgcacagcc cggccgcgcc
     1141 gaacagggcg gctacaccgt gatcgccttt cgcgtcccca ccgagtccga cacagcaggg
     1201 accgtcaagc tcgaggtcaa gctgcccgtc gaccacccga tcacctcggt ccgcacgacg
     1261 ccgcgacccg gctggaccgc gacgatgacc aagaccgggc tgaacccgcc gatccagagc
     1321 agtgggcgca cgatcaccga aaccgtcagc gccgtcacct ggaccgccga ccccggcacc
     1381 cggatcggcc ccggtgagtt cgccgagttc ccgctctcgc tgggcccgct gccgaccgac
     1441 accaaccagc tcgtgctccc ggccgtgcag acctacgacg acggcgaggt cgtcgcgtgg
     1501 gaccagccgc ccgccacgga cgggtccgag ccggaacggc ccgcgccgac cgtcgcactg
     1561 agcccggcgg cggcaggcga cgcagacgtc gccacgtcga cgaccggcgg gacgaccacc
     1621 accgcggcgg ccaatggcac cgacaccacc gcccgctggc tcggcggcgg aggcctgctc
     1681 gtcggggcac tcggcctcgg cgtcggagtg ggcgccgtgc tccgcaaccg gcgcgcggca
     1741 ggagcaggcc gatgaccgcc cgcgtcctgc gcgccgccgc cgtcaccgtg ctgtgcgggc
     1801 tcgcgctgct gctcggcgca ggccaggcgc tggcgcacac ccggctggag agcagcgacc
     1861 ccgccgacgg cgcgagcctg gccgccgggc cgcagcgcgt ctcgctgacg ttcaacgagt
     1921 cgatgcagcc cggtttcacc acgctgacgg tggtcgggcc ggaccaggcc cggtaccaga
     1981 ccggggacgt caccgccaac ggtggcacgg tgagcatcgg ggtgcgcccg ctcggccccg
     2041 cgggccgcta cgagatcggc taccgggtgg tgtccgacga cgggcacccg gtcaccggca
     2101 gcgtcgcgtt caccctcacc acgcccggcc cgggcagcac tgccgctgct gcgagcccga
     2161 cggccaccgg cgccgccgcg gcccctgccc cgtccacgcc cgtcgcggcc gaggcccccg
     2221 caacccagga cggcgggggc gctccggtgt ggccgtggat cgtcggagcc gtcgttctcg
     2281 tcggtggtgg ggtggtcgcg gcgatgcggc tgggacgggg atgaccgcac ccggcggggg
     2341 tccgcgcccg gtcgaccggt acctgctgcc ggtcgcctgg gccgggctca cggtcggggc
     2401 atccttcctg gccagcgtgc tgaccggcgg gctggccggt tcgaccctgc ccgtgctggt
     2461 cggaataacg gtcacccgct cgggcatgga catcgccggg gtggcgtgcg tcgggctgac
     2521 cctgctcggc gcgctcctgc cacaggcccg cgccctgccg ggcagggcgc tgcgcgacct
     2581 gatccgggtg cggaccacgg ccgaccgctc actcgtcctg ctggccgcta cctggctggc
     2641 actcgtcctg gtcggcatct tgttccgagt cgcgtccgcg ttcggtcgtc cggtggccga
     2701 actgaccggc ggcgaggtgg tggagtgggc gacagagctc gctgcaggcc ggggcacggt
     2761 gttgaccgcg ggctgcgcgg ccgtcgtcct cgggtgcgcc gtcgcccgcc tgcacgaccg
     2821 caacctcgtt caggtgcgga tcccgatggt cgccgcactg ctcgggttga tcaccccggg
     2881 agtgacgggc cacaccggct cggcgtcgga ccatgacctc gccgtcatct ccgtgggggc
     2941 gcacgcgggc gccgcggcgc tgtgggtcgg cggactcgga gccgtgctgg tgctcgtcgc
     3001 gcggcaccgg cccctgctcg atgccgtgct cccccgcttc tcccggttgg ccggcctgtg
     3061 cgtggtgacg atcacgctga ccggtctact gagcgcccag ttcgggctgg ggagctgggc
     3121 ggcgctcgtc agcaccggct acggccggct cgttctcgcc aaggccggct gcctggtggt
     3181 gctcgcgtgt ctcggcgggc tggcccgacg ccggctcgcc gccggacgca ccccggtgct
     3241 gcggtgggca gggctcgaga ccgcgctgat ggcggtgacc ctcgggctcg ccgccgcgct
     3301 gagccaggcg gcgtgacgct caggacgcgt caggcgggct ctcggtaggc ctccaggagg
     3361 cgcagccaca cctcgctgat cgtcgggtag gccgggaccg cgtgccacag ccggcccatc
     3421 ggcacctcgc ccacgatcgc gacggtggcg gcgtggatca tctcggccac atcctggccg
     3481 acgaacgtca ccccggccag gatgccgcgc ccctcgtcga ccacaagccg cgccgccccg
     3541 tcgtagccgt cggcgtgcag cgacgagcca cccacggcga tcgggaggtc gaccaccctg
     3601 acgtcgatcc cggcggcgcg ggcctgggcc tcggtgcggc ccaccgaggc gacctcggga
     3661 tcggtgaaca ccacctgcgg cacggcggcg tggtcggcgg tggcgacgtg ctcgccccaa
     3721 ggccgggtgt cgagctccgc accgatcgaa cgcgccgcga tcacggcgcc gacgatccgt
     3781 gcggcgtact tcccctggtg ggtcagcagc gcccggccgg tgacatcacc ggcggcgtag
     3841 agccactccc cctcgacacc ctcgaccaac cctgtgtcgt ccaccgtgag cggcttcccc
     3901 ggctccagcc cgacggtctc gagtcccacg gagtcggtgt tcgggcgtcg accggtcgcc
     3961 acgagtagct cgtcgaccgt gatcgtcgcg ctcccgacat gcatcgtgat cgggtcgccg
     4021 tccggcgcgg cggagtcgag cccctggtcc agccacaggt cgatgccatc ctcgcgcaac
     4081 gcggcagcca cccgctcgcc ggcgaacggc tccatggccg gcagcagccg aggctcgttc
     4141 tgcacgagcg tgacctccga gccgagcctg cggaacgcct gtgccagctc gcagccgact
     4201 accccgcccc cgagcacgcc gagccgggcc gggatctcct tggccgaggt cgcctcccga
     4261 gaggtccacg tgcgcaccgt gtccaagccg ggcaccggcg gccgcaccgg gacgctcccc
     4321 gtgcacagca cgacagctcg gcgggcggtg agcgtctggg caccggccgg gccgccgacg
     4381 acctcgaccg tcttctcgcc ggtgagcctt ccgtgcccgc gcaccagtgt gatcccggcg
     4441 cccaccgccc actccacctg gttctcgtcg ttccagttgt tggtgaagga gtcgcggcgg
     4501 gcgagcacgg cggtcgggtc gaagtcggcg gtcgtcccgg gcagcctgcg caccgcggct
     4561 accgcgtggc cggtgcgcag cagcgccttg gacggcatgc acgcccagta ggagcactcc
     4621 ccgccgagca gctcggcctc cacgatcacg gcagagagcc cggcacgggt ggcgtagtcg
     4681 gccgcgttct cacccaccgc gccaccaccg acgacgacca cgtcgaacgt agtcgccgca
     4741 cgctcgccct gcaccgtcat cccgacaccg ccgcgaccgc ctcgtgcacg tcgtggtcac
     4801 cctcgcgcag ctccagcacc cggcgtgcgg tgcgaggggc gtcgagcagc gccgccagca
     4861 cggcggcggt gtcgtcgcgc gtgacgtccg agcgttcgac cggcggcggg gcgagcagca
     4921 cccgcccggt accggggtcg tcggtcagcc ggccgggccg caggatcgtc cagtccaggt
     4981 cggtggcccg cagcgcctcc tcggcagcct tcttcgccct caggtacgcc gcccacacct
     5041 cgtcggtacc gggttccggt ggggcgtcca ccccgggaga ggagacgagc agataccggc
     5101 gcactcccgc aagctgcgcc gccgtggcca gcagctcggc ggcagcccgg tcgacgctgt
     5161 ccttgcgggc cacgccgctg ccgggcccgg cgccggcggc gaacacgacc gcgtcggcgc
     5221 ccgtcaggtg ggcggcgagc tcgtcggcgc tcgcgcactc caggtcgacg accgcggccg
     5281 acgcccccgc tcgctcgagg tcggcccggt ggtcagggtt gcgcaccacc cccgtcacca
     5341 cgtcgccgcg accggccagc aacgcggcca gtcgcaaccc gatcttcccg tgtgctcctg
     5401 cgatcaccac ctgcacaggc gagacggtag tcgcgcggca ccgggggcgc cgggcacgtg
     5461 acccatccgg gagcccggtc cacctgcggc cggtgcggcc ggtccgggcc gcggcagggt
     5521 ggtcctaccg gttcgggcgc agggtccaga ccacggacat ccgggtggtc tcgccgccgc
     5581 cggcgtcggt gatcacgacg gtcacggtga actccggccg cgtcccggcg ttgagctcgg
     5641 cgagcacgtc cgccgccggc cggtccagca cggcggctgc ggtgagggtg ccgcgggcca
     5701 gcctcacgta ctggatctcg gagcggacca ccagcggcgt ggctcggtcc atcgatgcac
     5761 cgaaggccgc catcgccacg gcgccggacg cggtctcacc cagaccgaag atcatcgctg
     5821 cgtgcgggcc gcccacgtgg ttgtgcatct cggcagcgtc cgggaggtac gccaccgcgc
     5881 gctcgggcgt gacctccccg aactcgacgc ccgtggtccg gacccacggc accgcctgtc
     5941 ccatcgccgt accgacccag cccgcgtccg tcgtcatcgg ggcgaggtta ctcgtcagta
     6001 gcaccccgcg ggaagaagcg gactgattcc gcgtgtcggc aggccggttc actgcaccgc
     6061 ttcaccatca gcgctggtca gctgcacgtc agcggcccac cgacgatgtc cgagatgtcc
     6121 gttttctcgc tccgcggaat cggggcggtt gtgagcggca ctacccagga ttggaatcga
     6181 ggcgcgggtt cgcatcgagg actgacatcc acggaggtat gccggtgaag gccgtccggg
     6241 tacacgagta ccacgtcgat ccaacgatcg acgacatacc cgaaccgaag atcacggggc
     6301 cgctcgacgt gatcgtcaag atcggtggcg ccggggtctg ccgcaccgac ctccacatcc
     6361 tggaagggca gtgggcggac gcgatgaacc cgcagctgcc ctacgtcatc ggccacgaga
     6421 acgccggctg ggtgcacgag gtcggtgagg gtgtcaccaa cgtcgcggtc ggcgacacgg
     6481 tgatcctgca tccgcagccg tcctgcggac tctgcctggc ctgccgggcg ggccgggaca
     6541 tgcagtgcga gaacgggttc ttcccggggt tgtccgacaa cgacggcggc atggccgagt
     6601 acctgcgcac caccgcccgg gcctgcgtga agctcaaccc cgacaccaat cccgccgacg
     6661 tcgccgcgct cgccgacgca ggcatcaccg cctatcacgc ggtgcgcaag gccgtgccgt
     6721 ccctctatcc gggcacgact gccgtcgtcc agggcgcagg cgggctcggg cacatcggta
     6781 tccaggcgct tgccgcgctc accgcgaccc gcatcatcgc cgtcgaccgg aacccggagg
     6841 cgctggcgct ggccaagcag atcggcgccg acgagaccgt gctggccgac ggcagtcaca
     6901 tcgacgccgt gaaggacctg accggcggca acggcgcgaa cgtcgtgttc gacttcgtgg
     6961 ccgagcaggg cgcggagaac gacgcgtggc agatgaccgc gcccgccggt tcggattaca
     7021 tcatcgggta cggcggtgag ttgcggatcc cgaccctgga cttcgtgggc ggcgagaaga
     7081 acgtcatcgg caacatcgtc ggtacctaca acgatctcgc cgaactgatg gtgctggcgc
     7141 aggccgggaa ggtcacgctg cataccaggc aatatcccct cgatgcggcg ctcgacgccc
     7201 ttcacgatct cgacgccggc cgggtccgcg gccgggcgat cctcgttcca tagccctcga
     7261 tatccagctg gtccgcatcg acgcgaaagg cccaccacat gtacgagaag gacggcgaga
     7321 agtacttcat cgtcgactca cacagccatt tctgggacgc gagccgggag aactggaagc
     7381 cgggcgcgga ggagtacgcc aagggctgga tcgactgctt ctacggctac caccagctcg
     7441 gcccgcccga gacccactgg gactacgaga agtacctcaa ggtcacgccc gaggacttcg
     7501 agcgcgacat gttcatcgag ggccacgtcg accacgcgat cttccagtcg acgtacctca
     7561 aggagtggta caccaccggt ttcaacaccg cggcgaagaa cgcggagctg ctggaacggt
     7621 ggggtgaccg cctcatcgtg aacggccgct tcgacccgcg cgacggcgac gccggcatgg
     7681 ccgagctcga ggcggacgcc gagaagtacg ggctcaaggg ggtcaagctc tacaccgcgg
     7741 agtggaacgg tggctcccgc ggttggaagc tcacggaccc cgagtcgtac cggttcctgg
     7801 agaagtgcca ggaactcggc atcaagaaca tccacgtcca caagggcccc acgatctggc
     7861 cgctgaacaa ggacgcgttc gacgtcgcag acgtggacca gaccgccacc gacttcaccg
     7921 atctgaactt catcgtcgag cacgtgggcc tgcctcgcat cgaggacttc tgcttcatgg
     7981 cggtgcagga gcccaacgtc tatgcgggtc tgtccgtggt gatcggcggg ctgatgcacg
     8041 cccggccgaa gttctttgcc aaggtcatcg gtgagctgat gttctgggtc ggcgaggaca
     8101 agatgctctt cggcggcgac tacaacatct gggtgccgaa gtggcaggtc gagggattcg
     8161 tcgactggca gatgccggac gacgaggcct tcaccgacta cgcgaagctc acgaccgaca
     8221 cgaagaagaa gatcctcggg ctcaacgcgg cgcggctcta cgacatcccc gtgccggccg
     8281 agctccagct gtcgaccaca gcgggcgcag acctgcagcc gggcgaggaa ctggtcaagg
     8341 acgtcgcggg ggcgcaggcg tgacgccgcg cccacccggt tccgaccttc gggacgcggt
     8401 gtgggccgcg ctggggacgg tgctcgaccc ggagctggac gagcccatca cggacctcga
     8461 cttcgtcgag tcgtgcaccg tctccgagca cgacactgcc gacgctcccg gggtggtggc
     8521 gacggtcgtc ctgcgcctac ccaccttctt ctgcgcgccc aacttctcgt tcctgatggt
     8581 cgccgacgcc ttcgatgcgg tgtcgacggt cccgggggtg aggcgggcgg acgtgacgct
     8641 ggccgaccac cacgcctcgg cggagatcaa cggcggggtg gcggcgcagg ccgggttcgt
     8701 cgggacgttc gggagcgaag ccgctgccga actggacgag ctgcgcgcgc agttcctccg
     8761 caaggcagcg ctcgccggac aggaccgggt cgcccggcca ctggtggacg ccggcaccgg
     8821 ccccgacgag ctcgcggagc tgaccctcgg ctccgtgccg ccgtcaccgg acctcagcag
     8881 gctccgggag cggcgggcgg cgctcgggat ggcgcacggc gacgaggcgc cgctgctcgt
     8941 gcacgccgac ggcagccggg tcacggccgc gcaggtgccg ttgcacctgc ggcgggcgcg
     9001 cttgcagcgg atcgggatcg agacgaacgg ggagtactgc aagagcctcc tgcagaccag
     9061 gtaccccacg gcagtgcgct gacgcgcccg tgcgcggata cgggtgctgg agcactcgta
     9121 tccgcgcacg accgcggtcg tagggtcacc tgatggatgt ccgcgcgctc gtgttcgacg
     9181 tgttcggcac cgtggtcgac tggcgctccg gggtggcgcg agagatcgcg cggctgctcg
     9241 gtgagcgggt tgacgccgac gccttggccg acgcgtggcg cgggcgctac ctcccgtcga
     9301 tggaccgcgt gcgccgcggc gagctgccct ggacttcgct cgacgggttg caccgggcct
     9361 ccctcgacga gctgctcggc gagttcggcg caccggagac gccagagcag gtccgcgacg
     9421 agctggtgct cgcgtggcac cggctcgacg cgtggcccga ctccgtcgaa ggcctgacgc
     9481 tgctcaagcg ccaccacgtg gtcgcctcgc tgtccaacgg caacgtggca ctgctcgtga
     9541 acatggccaa gcacgccggg ctgccgtggg acaccgtgct ctcggccgag ctgttcgggc
     9601 actacaagcc cgacgcggag gtctacgacg gcgccgcacg gctcctcgga ctccggccgg
     9661 agcaggtgct catggtcgcc gcccacaccg acgacctcgc cgccgcccgc tcgcgcggcc
     9721 tgcgcacggc ctacgtccac cggccgctgg agcgtgggcc ggacgcgccg ccgcccccgc
     9781 cgaccgaccc ggatgccgat ttgaccgtcg ggtcgatgac cgagctggcg caccgcctca
     9841 cctcctgacc ccccgcccct cctgcgccac ggtcgccagc acccggaacg tgttcacgag
     9901 gcggtagccg ccgtcggcag tgcgctgcgg ggagagccgc tcgagtaccg ccctgcggac
     9961 ggccggttcg cccgcccgcc cgactgcctg ccgcccgatc ccggacatga gcagtggccc
    10021 gagcagcgcg tcggcatcgg ggtagtcgaa cgggcacgcc acctcgtcga acacgtcgac
    10081 cgtcaaaccg gcgagcgtga ccagcttgcg caggcgggca ggctcggtga gcggtggcgg
    10141 gccggtgggc gcccgccgcg gtggcagcca cggggcgagt gcctcgccga acgcgcgcac
    10201 gtcgcactcg tcctcccgcc cccataccgt gaggacgacg gtcgagccga cccggcccgc
    10261 cgaccggagc acggcgagcg ggttggtgac gtgggacagc agctgcacgg ccgcgacgac
    10321 gtcgaacggc ccggcgggtg ggtcgtgtgc gtccccgacg cgaaaggttc cctccggcac
    10381 cgcggccgcc gcagcgacga cggcggaacg gtcgacgtcc acccctgtga cgacggcact
    10441 gcggtcggcg gcggccctgg cgaaggcccc cgcaccgcag ccgaggtcca gcagcgtgca
    10501 gcccgggccg acgccggccg cgtcgagcgc ccgtgcgtgc agtggcgctc cccagccgcg
    10561 cggaccttcg tcggacacgg ccgcgaggct accgacgcgg cccggccggt cagccccaga
    10621 cgtcctgcgc aaccttgacg atcaggcgca gcttcgccac ctgctcctcc tgcgtgagcg
    10681 agttgccctc cacggtggag gagaagccgc actgcgggga gaggcacagc tggtcgagtg
    10741 gcacgtactt ggacgcctcc tcgatccgcc gcttgagcgc gtcggcgtcc tcgagctcgc
    10801 cgcgtttggt ggtgacgagc ccgagcacca cctgcttgcc cggcgggacg aaccgcaacg
    10861 gggcgaaccc gccggaccgc tcgtcgtcga actcgcagaa gtagccgtcg acctcgaggt
    10921 cgccgaacac ggcctcagcc acgaagtcgt agccgccctc cgccgcccac gacgaccgga
    10981 agttgccccg gcacatgtgt gtggtgatct tcatgtcggc aggtcggtcc gcgatggcgg
    11041 cgttgatctg gcggatgtag cgcaggtggg catggtcggc gtcctcgccc ttcgcggcca
    11101 tctcggctcg ctgcgccggg tcgttcaggt aggccaggct cgtgtcgtcg agctgcaggt
    11161 agcggcaacc gaggccggcc agcgcacgca cctcgtcggc gtaggcggcg gacaggtcgg
    11221 cccagaactc ctcgacgtcc ggatagacac ccttgtcgag cgccgccgtg ccaccccggt
    11281 agtggaccat gctcggcgac gggatcgtca gcttcggcac ggccgtggtg gtgatcgacg
    11341 cgaggaaccg gtagtggtcg gcgaagatcg ggcgctcgag gcgcaccttg tcgtggatgg
    11401 cgagcccggc cgacgtgaag gcgccctcgc cttcggcgtt gcgcatccgc acgcgaatct
    11461 gctcgtcggt cttgctgacc ccgccgagct ggtagatgaa gtccatgtgc cacgacgtgc
    11521 gacggaactc gccgtccgtg gccgcctgca ggccgatctc ctcctgcatc gccaccgcgt
    11581 cccggatcgc ggtgtcctcc gccgcgcgca gaccgtcggc gtccaggttg cccgcggcga
    11641 actgctcccg cgccgcgagg aggttctgcg ggcggagcag gctcccgacg tggtcggccc
    11701 ggaacggcgc ctcagtgcgt ccatgcatgc ccggcaactt atcccgggtg cccgtgcgcg
    11761 cccgcgtgac cttccgcatg atggaaggag cgttctcggc caccgtggaa gtctggcggg
    11821 atgcagcacg gcatcgtcac gttcctgacc gactacggca tcgatggccc gaccctggcc
    11881 acggccgtgg aagagcgcgg tctcgacgcg ctcttcctca cggagcacac acacatcccc
    11941 accagcctga agacgcggtg gccgggcggc gaggacctgc cgcggcacta ctcgcacacg
    12001 ttcgacccgt tcgtcgcgct cgcggcgatg gccgccgtca ccgatcggat cgcactgggc
    12061 acggccgtgt cgctcgtgct ccagcaccat ccgatcggcc tggccaagac cgtcgccagc
    12121 gtggatcacc tcgcggacgg tcgattcgag ctcggcgtcg gcccggggtg gaaccgcgag
    12181 gagatggcca accacggggt cgacccgcgc acccgcacgg cacggatgct ggagcacatc
    12241 gcggccatgc gggcgatctg ggagaacgac gaggcggagt tccatgggac gttcgtcgac
    12301 ttcgacccga tctggtcctg gcccaagccg gtgcggatgc cccccgtcct gatcggtggc
    12361 gagggcccga cggtgttgga ccgcgtgctg tcgcacggag acgcctggct cccacgacgc
    12421 ttcgcacccg aggagatcga cgtcctcgcg gcccggatcg ccgagctgcg gagccgggcg
    12481 gccgatgccg gacgcgggca catccccgtc accatgtacg gcggcgaccc ggaccggaag
    12541 gcgctggact cctacgcagg agccggcgtg gatcgggtgc tgttctcgct gccggccgca
    12601 ggacccgaga ccgtcctgcc cggcctcgac gagatcgccg acctggtggc gtaaccgcac
    12661 acgtgagacg tcagcagggc catcccgccg acgcccggtg ggccgtttcc ccgacgcgtg
    12721 ccgcggcctg ccgcaccacc cgttccagat cccagcgtct ggcggcgaag gccgcccgcg
    12781 tcaccgagat gccgagagca cccgtgacgt ccccgcccgg accgcggacc ggtgcggcga
    12841 tgccggccac ccctggctgg tactcctcaa cctcgacggc ggcgccgtcg gagcggaccc
    12901 gcatcagctc gcggtccagc gccctgcggt cggccacggt gcgcggagcg agcctgggca
    12961 tcccggtgcg ggcgatgagc tccgccagcc gcccgggacg gaggtcggcg agcatgacct
    13021 tgccggctgc ggtggcgtgt ggcgcggtgg gttcgccgac gcgcatcccg tcgggacggg
    13081 ggtggtcggt gcagtcgtcg acgtacgcca ccacgatgtc gacgtcgcgg agcaccgcga
    13141 ggtaggcggc ggcctcggcg gcggtgtgca cctcgtggag gatggcgcgc acggacgcgg
    13201 gcgggcgcac ctggtcggtg aggccgcggt gcagctcggc gacgcggtag ccgagtccgt
    13261 agccgcggac gtcgtgcagc cggaccaggt agccctcctc gacgagcgtg ccgagcgcgc
    13321 ggtagacggt ggggagcgga gccccgagcc ggcgggcgat ggccttcgcg gtgacgccgt
    13381 cgcccgcagc ggcgaccgcc tcgacgaccc gcagggcgcg ggtgatcgag gaaccggtcg
    13441 tgcgcgccgg tgccacaggt tccagtgtcg ggtagaacgt gcgtagcgcg cacctcaccg
    13501 atggacgggg acgacctgat gaacgacgac gagctgcatc gccagatcga cgacctggtg
    13561 gccgaggagc accggctgga gcgcgcccat gtcgggcagc ccctgtcgga gtccgagcag
    13621 cagcggatgg agcagctcgg cgtgcagctg gaccggtact gggacctgct gcgccagcgc
    13681 gacgcacgcc gccgcgccgg cctggacccc gacgatgcac aggagcggtc cggcgatgtt
    13741 gtggagggct accgccagta gccgaccgcc gggcatcacc gggcatgccg tcgaccttgt
    13801 tcggggtgcg ccggacgccg ggttcgggga gcatcgacgc gtgcagatca ctcccgtcag
    13861 cccggacctg ctcgtcgagg aggtgacggg cctcgtcgcg gcgcggccgg ggcgggtacg
    13921 gctcgcgctg gacggaccac ccccggcccg gcccgtcgcg ctcgccgagc gagtggccgt
    13981 ggagctccgg gcgctcggcc gcgcggtgct ggtggtagcc gccgccgact tcctgcggcc
    14041 cgcgtcggtg cggctggagt acggccgcga ggatcccgac gagttcctcg acggctggct
    14101 ggacgtgggc ggcctgcgcc gggaggtgct ggacccggcc gggccggacg gctccgggcg
    14161 ggtgctgccc cggctgtggg acgccgcggc ggaccgcgcg taccgggacg ggtacacgga
    14221 gctccccgca gacggggtgg tactgctcgc cggcgctctg ctgctcgggc gcggactgcc
    14281 gctcgacatg agcgtgcatc tgcggatgaa cccggccgcc ctcgcccgca cccttcccga
    14341 tgacgaacgg tggacgctgc cggcctacgc ccgctacgac gccgaacacg atccggcgcg
    14401 agccgcagac ctgctggtgc tggtcgacca ccctgaccga cctgccatcc gacggccgga
    14461 aagaggtgat tgtgggcgag gagggagttg aaccctcacg tcctttcgga cacacggacc
    14521 tgaaccgtgc gcgtctgcca ttccgccact cgccctgaca gaggaaagtt agcacggggc
    14581 cgacaagccc tgatctgccc cccaggtcgg ggggcgcagc gctgctggct agcacagata
    14641 cgatctgtgc tgccagcaac acgccgatgc gaacggagga agatcggttg ggccgcgtcg
    14701 ataggttcga acgccgtctc cagggactgg tgggcgacgc cttcgcgcgg gtcttcggtg
    14761 gcagtgtcgt tccccaggaa gtcgtgcaag cgctgctcag ggaagccgag gaccacatcg
    14821 aggagctcgc cggaggtcgc ctgctggcgc ccaaccggtt caccgtgctc ctctgcccct
    14881 ccgatctcga tcgcatggcc ggcgaccgga cagagatcgt gaacacgctc tctgcctcgg
    14941 tcaccgaaca actcgccgac cagggatggg acacctacgg cgaggtcgta gtctttctgg
    15001 agcgctccga tgcgctgcac acgggacagt tccgcacccg ctcgtccgtg gaccccgacg
    15061 cttcccgccg ggacgccatc cgagcacgcg acgcaggaga agcacccatg agccagcaac
    15121 cgggccaccc cgaggagaac gggcagtacg gctacgaccg tggcgccccc ggcggatacg
    15181 ggcagcagac gtacgccccg cccgcctacg accagcgtca gggggggtac gaccagggcg
    15241 agcagccacc gcagcagccg ggctacggcc agcagcccgg ctacccgcag cagggcggcg
    15301 gcggcggcta cccgcagcag ggcggctacg accagggcta cggccagcag cagggtggcg
    15361 gctacccgcc gcagcagggc ggtggctacg gcggccagca gggcggtggc tacgagcagg
    15421 gctacggcca gcccggccag cccgggcagc ccggtcagcc cggctacccg cagcagggtg
    15481 ggggctacga gcagggctac ggtcagcccg gctacggtca gcccggttac ggccagcccg
    15541 gttacgcgcc cgggtacgac gccggctacc agcagggtgg ctaccagcag ccctacgaac
    15601 agggctacga cgcgggctac gaccagcagc agcagggcta cgggcagcag ggcggctacg
    15661 gcggctacca gcaggggctc tccgcgtcgc tcagcctcga cgacggctcg ggtcgcacct
    15721 acgacctcac ccagggcagc cacgtcatcg ggcgcggcca ggactcgtcg ttccggctgc
    15781 cggacaccgg cgtgtcgcga cgccacctgg agatcagctg ggatggtcac actgccacgc
    15841 tgacggacct cggctccacg aacggaacca ccgtcaacgg caatccggtg cagacctggc
    15901 agctgggcga cggcgacgtc atccgggtcg ggcactcgag cctcgtcttc cgcacgcagt
    15961 agccgcgggc ccgagctcgg ccaacagcaa tcatcggaga gaacggagtt cgccgcgagt
    16021 gccggagttg gtgctgcagc tgaccagggc gggcttcctc gccctcctct ggctgttcgt
    16081 gctggtcacg ctccgggtgg tgcgttccga tctgtacgcc gcgtcgggcc tgcgggccct
    16141 ggtccccggc ggtcggaccg ccacccggac cgggcgcggt cgcacggccc gccagctgct
    16201 cgtgacgcaa ggagcgctgg cgggaacgcg agtgtcgctg gactcccgac cgatcctcat
    16261 cggtcgggcc gacgactcga cactggtgct cgacgacgac tacgcctcca cccggcacgc
    16321 acgcatcgcg ttgcaggggg aggagtggta cgtcgaggac ctcgggtcga cgaacgggac
    16381 gtaccttgat cgggctaagg tcaccggacc cacccgagtt cccctgggag tccctgtccg
    16441 catcggcaag acggtgatcg agcttcggtc atgagccggc gagcgctgca gtgccgcacc
    16501 ctggcatccg accggggacg gcctcgccgg ccgatctgcc ccgaacgtat tggcggagcg
    16561 ctgacatgac gcttgtcctg cgctactccg cccgcagcga ccgcgggctc gtccggcaga
    16621 acaaccagga cgcggtctac gccggcccgc ggttgctggc cctcgccgac gggatgggcg
    16681 ggcacgcagc gggcgaggtg gcgtcctcac tggtgatctc cgcgttcgca ccccttgacg
    16741 aagacgaccc aggcgacgac ctgctcgccg agctgcgcca cgccaccgtc gagggcaacg
    16801 ctgcgatcac gcggcacgtc gccgacgcac ccgacctcga cggcatgggc accacgctga
    16861 cggccatcct gttcgcggga tcgcggctgg gcctggtgca catcggcgac tcgcgcgcct
    16921 accagctacg cgagggcgcc ttcacacaga tcaccaagga cgacacgttc gtccagtcgc
    16981 tcatcgacga gggccgcatc accgaggagg aggcgcacac gcacccgcag cgctcgctgc
    17041 tgctgcgcgc catcaccggc caggacgtcg acccgtcgct gacgatccgc gaggcgcgtg
    17101 cgggcgaccg gtacctgctc tgctccgacg ggctgtccgg cgtcgtcagc gacgagacgc
    17161 tggccgacac gctgcaggcc taccgcgacc cgcgggagtg cgccgatcgc atgatcgagc
    17221 tggcactgcg cggcggcggc ccggacaaca tcacgtgcat cgtcgcggac gtcgtcgaca
    17281 tcgagttcgg cgaggacgcc ccgatcatcg gcggatcggt gggcgacggc agtgaggacg
    17341 gcccgcggcc ggactccgcg gccgcccgtg catcggccac caccctcacc cgcgtggccc
    17401 cgcagcgggt agtggctgcc acgaaggcgc ctgcggcccg tcgctcccgc gtgaagatcg
    17461 tgatgggcgt ggtcaccgtg ctcgcggtgc tgatcggcgg ggtcgtgctc gcccggatgt
    17521 gggtactgca gcagtactac gtcggcgtcg acgcgcagct ggtctcgatc ttccaagggg
    17581 tgcacggcga ggtgctcggc atcccgttgc agagggtggc ggagcgcacc gacatcaccc
    17641 tcgacgacct cccggagacc gatcgcagcg ccgtccgtga cgggatccgc gccctcgacg
    17701 ggctccccgg cgcccgcggc gtcgtcgaac gcctgcgcgg ccggatgctg gagccctgcc
    17761 cgccgccggc gcctccgcag ccgccacccc cggtgcccga cccgaacctg ccacccgtcc
    17821 cgggcgccgc gccgcctgcc gacacgaccc cgctgccgac gccggcaccc gagcccggaa
    17881 cgacctgccg gtcggtgagc tgatggcagc tcccgacacg cgctccgaac cggcgcctct
    17941 cgccccggcg ccactgccca acgggcgcgg catcgagctc gtcctgctgg cgttcgcggc
    18001 ggtgctggtg acgggcgcgc tcgtgctggt ggaggcgaac caggagcagc agctcacgcg
    18061 gtcgttgctg ttcgtcgggc tcgcgtatct gggcctgttc acgatcgctc acttcgcggt
    18121 gcgcaggttc gccccgttcg ccgacccgct gatcctgccc tgcgtggcac tgctcaacgg
    18181 gctcggcctg gtgatgatcc accggctcga tctggccgag gtcgaccagg cgctggcgct
    18241 gggcagggac gtgcctaccg agctcatcgg caggcaggtc gcctggaccg cgatcggtct
    18301 cgcgttgttc gtcgccgcgc tgtggctcgt gcgggaccac cggacgctgg cccggtacgc
    18361 gtacaccgcc gggttcgcgg gaatcgtgct gctggcgctg ccaggcctgc tgccgtcgtc
    18421 gatctccgag gtcaacgggg cgaagctgtg gatcagggtg gggccggtcg gcattcagcc
    18481 cggcgagttc gccaagatcc tgctgatcgt cttcttcgcg gcgttcctcg tgcagaagcg
    18541 cgacctgttc accaccgcag ggcgccggtt cctgggcatg gaactgcccc gtgcccgcga
    18601 cctcgcgccg ctgctgctcg catggggcat gtcggtcggc gtgctcgtgc tcgagaacga
    18661 cctcggcacg tcgctgctgt tcttcggcat cctgctggcg atgctctacg tggccaccga
    18721 gcgcgtgtcg tggatgctca tcggcctgat gttcttcttc gccggatgca cgatcgccta
    18781 ccatctgttc gcccacgtcc gagtgcgcgt acaggtctgg ctggacccct tcggcgactt
    18841 cgccaacacc ggatatcaga tcgggcaggc tttgttcagc ctcggcaccg gtggcgtcgg
    18901 cggcaccggc ctcggagcgg gccgtccgga cctcgtcccg ttcgcggaga gcgacttcat
    18961 gctctcctcg ctgggtgagg agctggggct gatcggcctg gccgcgatcc tcgtgatcta
    19021 cctcgtgctg atcacccgtg gcctgcgcag cgcgctggcg gtacgcgaca gcttcggcaa
    19081 gctgctcgcc gctgggctcg cgttcgccct cgcgctgcag atcttcatcg tcgcgggcgg
    19141 ggcgtcgaag ctcatcccgc tcaccggcct gaccctcccg ttcctgtcct acggtggttc
    19201 ttcgctcgtc gccaactacg cgctggtcgc gctcctgctg cggatctccc acgccgcgcg
    19261 ggccccgctg ccccgccggc cggccacgcc gctaccgccg atcgccgagg ccggcaccga
    19321 gctggtggag cgtccgcggt gaacactccc atccggcgca tcgcaatcgc ggtgatggtg
    19381 atgatcctgc tgctgatgtc caacctgacg tacgtgcagg ttgtcaaggc gggcgactac
    19441 cgcaacgacc cgcgcaacca gcgggtgctg ctgtcggagt actcccgcaa gcgggggcag
    19501 atcagcgcca acaaccagat cctggccagc agcgtcgaga ccaacgaccg gctgcgctac
    19561 cagcggcagt actcggacgg ggccgtctac gggccgatca ccggctacta ctcggtcatc
    19621 tacggctcca gcggcatcga gcgcgcctcc gattcggtgc tcaacggcag cgacgaccgg
    19681 ctgttcggcc gcaggctctc cgacctcatc accggccgcg accccagtgg cggcaacgtg
    19741 gtgctcacga tcgaccccaa cgtgcagcag accgcctacg accagctgca gcgcaacggc
    19801 tacacggggt ccgtggtggc gatccgcccg cagaccggcg agatcctcgc gatggtctcc
    19861 acgccgtcgt tcgacccgaa cccgctggcc agccacaact ccgaccagca gaactcggcg
    19921 tggaaggccg acagggacgc caacccgccg gtgctgatca accgcgcgat ctccgagacc
    19981 tatccgcccg gctccacgtt caagctcatc gacaccgccg cggcgctcag tagcggtcgc
    20041 tacacacccg acagtcagct caccgcggcg ccgcgggtca cgctccaggg caccaacacg
    20101 acgctggaga acttcaacgg caatgcgtgc gggacgtccc cgacggcgag cctgcgcgat
    20161 gccctgcagc gctcctgcaa caccgccttc gcaacgctgg gcgccgacct gggcgagcag
    20221 gcgatccgcg accaggcgaa cgcgttcggg atcggccaga gcctctcgat cccgatgccg
    20281 gtggcaccct ccacgatcgg cgacatcccg gacacggctg cactggagca gtccgcgatc
    20341 gggcagcggg acgtcgcgct caccccgctg cagaacgcga tgatcgtggc caccatcgcc
    20401 aacggcggcc aggtcatggc gccccacctg gtcaaggaga tccagagcca ggagctagac
    20461 ccggtcgcga ccaccgaacc ggaccggctc ggccgtgcca tcgaccccac cgtcgcgcgc
    20521 accctgaccg atctcatggt caacaacgag aacagctacc agggcgccgg caagatcacc
    20581 ggtgtgcaga tcgcggccaa gaccggcact gcggagcacg gtaacgatcc caagaacacc
    20641 ccgccccacg tctggtacgt ggggttcgcc ccggcgaaca atccccaggt ggccgtcgcc
    20701 gtactcgtgg agaacggcgg cgaccggaac aacctcgccg ccaccggtgg cgcggtggcc
    20761 ggcccgatcg gccgtgcggt gatccgggcc gcgctcgggg gtggacagtg agcacccgac
    20821 ggcccgccac gacatcattc ggcattccga cgtccggtac cgccgtgccg acgagcgcga
    20881 gcgaggagca gtcgtgacgg ttctcgcggc cgggcagaag attgccgacc gctaccacct
    20941 ggaccaccgc atcgccgtcg gtgggatggg cgaggtctgg gaagcctccg
//
LOCUS       DASRSC010000262        12031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2394503,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000262 DASRSC010000000
VERSION     DASRSC010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12031
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2394503"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VFE39_12880"
     CDS             <1..211
                     /locus_tag="VFE39_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259538.1"
                     /translation="SHTIALRANVAGCAALLGRWLEAAAGFERAAADSAAVLGHQHPD
                     TVALQDDLRNVHDPAYFAIPAGVSV"
     gene            complement(243..1031)
                     /locus_tag="VFE39_12885"
     CDS             complement(243..1031)
                     /locus_tag="VFE39_12885"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6259539.1"
                     /translation="MRESAARHDRTGHETKLVLDDLRKAYPGPLPVLDGVTFDVAPGE
                     FVSVIGPSGCGKSTLFNVIAGLERPDSGRVLVDGVDATGHVEPFAYMPQQDLLFPWRT
                     VLDNTTLGLEVAGMSRRAARERARPLFAEFGLTGFESAHPYELSGGMRQRAALLRTVV
                     QDRAVLLLDEPFGALDSLTRTDMQTWLDGVRAHFDWTVLLITHDIREAVFLSDRVVVL
                     GPRPTRVRREVTVNLPRPRELAMITSPRFAAVEEELLGVLRDRS"
     gene            complement(1024..1812)
                     /locus_tag="VFE39_12890"
     CDS             complement(1024..1812)
                     /locus_tag="VFE39_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6259540.1"
                     /translation="MSATRTGWRRTVSAVVPSLVLVALGLVAWQWYVTAADVRPEILP
                     SPLRVATQGWAFRDQLWANTLPTLQVTAVGFGVSLALGWALAIAVDFSPWLRRAFTPL
                     LVASQTLPIIAIAPLLIIWFGFGLLPKVLVIALVTFFPIAVGLIEGFAATERQATNLL
                     RSMGASRWKQFRYVRVPGAMPSFFTALRIGITYAVTGAIFAEYVGATAGLGIFMSLQK
                     NSFRTDLVLAAVVVTALVSVTLFGLTYLVQRLVIPWHGKERRRA"
     gene            complement(1809..2897)
                     /locus_tag="VFE39_12895"
     CDS             complement(1809..2897)
                     /locus_tag="VFE39_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6259541.1"
                     /translation="MSGVRPPSPRTPHVLRARLGTARIVTVLVAVLAVVLAGCAGRGA
                     AGGGDAAGGPQTIRVALDWTPNTNHTGLYVAQQEGWFRDAGLNVQFLPYNNTSPDTLV
                     SSGAADFGISFQDSFTFSKAAGADITSVMAVLQHWATRIAVRADRTDINSPKDLDGKT
                     YGGFGGPGEQQKMQAVIRDAGGKGDFKTVVLGTSAYEALYAGQVDFTEPFMAWEGIEA
                     DLHGQPLKTFAYTDYGFPDAYNVLLIGNSPWLKAHPDLAAKFVQAAQRGYQLGADDPA
                     KAAQLLMDANPGVFTEPELVMRSQLMLSDQYLRVASGKVGEQTLEKWSGFSGWVFDSG
                     VLTGPDGKPLTQRPDFSTWFTDQYLAKP"
     regulatory      complement(2890..3002)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3212..3823
                     /locus_tag="VFE39_12900"
     CDS             3212..3823
                     /locus_tag="VFE39_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HET6259542.1"
                     /translation="MGASTARKCVEVPAGTGRAVHVTAGERIRVVDPAGGQVGDVFAF
                     AGAEHHSAAHTRAATSRLFPAVGEHFVTDRRRPILTLVEDTSPGQHDMLIAACDPERY
                     AALGAPGHRSCAENLRETLAAVGHDYTGPTPQPINVFMRIPVDGDGRLRWLSAPTAPG
                     DAITFEAVLDCVVVVSACPQDIVGINAGGLSPLAIEILPRNPS"
     gene            complement(3846..5465)
                     /locus_tag="VFE39_12905"
     CDS             complement(3846..5465)
                     /locus_tag="VFE39_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016335563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HET6259543.1"
                     /translation="MLSLGALLDDSALALRVLVPGRSGALAGEVLWVHITELPDPSPY
                     LRERELVLTNGLWLDGTDPADFVAGVVRAGAAGMGFGLRAQCTTTPQALVDACRSAEL
                     PLVEIAIDVPFTAVTRAAAAIYTEHRQDALVGMVRRGNALAAAISHGAGASGVLDILR
                     RDHDLPLAVVDRMGRLLGAAAGRLDEGELRIAADALARRPTPLEVDLGPRGPAALFPV
                     GAVGDADAALLCLRPAVALDRAERDALDQAARFLSLEVARQQTVQAIEQRFAGELLDM
                     ILSGAQRAAEVPARLRAFGVDPEAPLAVCAVAFRRRRGPERSGAAGSAAAEAHADPPT
                     LPGVAEAVTDFFLAEGLPAVVAGGSTDVVAVLPWRGPAHGVAAVAGRLAEAVARRFPG
                     ERPVVGLSDPAASAAKLRQPLVQSREACRVLRDRHGGADVATFAELGTYQLLLALQET
                     EQLQAFADSVLGPMRAHDRKRGGELEATLRAFLGHDGQWATTAAAMYVHVNTLRNRLN
                     RISELTGRDVGRTADRVDLFLALEADAVSSG"
     gene            5711..5848
                     /locus_tag="VFE39_12910"
     CDS             5711..5848
                     /locus_tag="VFE39_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259544.1"
                     /translation="MTTAMWLILAVGVFIGMYVGRWWAEIRRANYDMDRIWAMRRNYR
                     D"
     gene            complement(5866..7068)
                     /locus_tag="VFE39_12915"
     CDS             complement(5866..7068)
                     /locus_tag="VFE39_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020657977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6259545.1"
                     /translation="MNRTWGGDGGGGATGATSGARPDEARRHNRAALLRRLHVDGPCT
                     RATLATELGLNRSTIKAVVDGLAEAGVVTEALPAQRKGAGRPSLMVLPKPEAAVVLAV
                     DVRVEQVAMSMVGIGGQVLGRHSWNLHHRTRTPGEVITHVAESAQLLAEELAVTALGV
                     GVSVPGVVRRDDGFVHEAPNLAWREVALGARLVTVLKVPVQVANDAELGALAEHVRGD
                     ARNVDDLVYISADKGVGGGVISDGRPLRGTRGYVGELGHLVVRPGGRPCYCGSRGCWE
                     TEVGEAALCRALGLPEDTPRGVVVAELRSLATAPLPGPLDEFTEWLATGLVTVVNMMA
                     PELVVLGDLFTALPPAVVGEVRAKVHERSLVSRAVGGTRIETSPFGRDAKLVGAAELA
                     FEPILGAV"
     gene            complement(7065..8564)
                     /locus_tag="VFE39_12920"
     CDS             complement(7065..8564)
                     /locus_tag="VFE39_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6259546.1"
                     /translation="MTAPTEGPQSTQTEQKAEPGSTTPAAGTGAAERAAPASARAADF
                     GIDTTARTTGEALRGYLTSLRSGDLGALPALLGLLALFILFTALAPDTFPSLLNLANL
                     LQQGAGQTIIAMGLVFVLLTGEIDLAAGTASGLTASVMALHLVSSGNLLGAMGTPVFL
                     LFAAVIVVAIVLAVLLRIWAGAAAAAVALLLLVVGFAPNPWLEMLLAVCVGVVIGCLT
                     GFLVAKVGMPSFVVTLALFIAWQGVILQLIGDGGTLGLRDPVINAVANGNLSTLGSWV
                     LFIVAAGGYAAVMINRQVARRRLGLVAQPTGLVLIKTAAVVVLAAVATYLLTVNRSPG
                     QLQISGVPYVVPLVLVLLVAGTYVLDRTRFGRHVYAVGGNREAARRAGIDVVRIRAAV
                     FVISTAFAAVGAIVYSSKVGSVSPSAGGGNTLLFAVGGAVIGGTSLFGGRGRISNAVI
                     GGAVLATVNNGLGLLGQPASVVFLVNGIVLLLAAGVDVLSRRRSAVAVR"
     gene            complement(8564..9337)
                     /locus_tag="VFE39_12925"
     CDS             complement(8564..9337)
                     /locus_tag="VFE39_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6259547.1"
                     /translation="MTDPILQLRGVNKSFGAVQVLHDVDLTVRAGEVTALVGDNGAGK
                     STLVKCVAGIHPIDSGEVLFEGRPVTMNAPTDAAKLGIEVVYQDLALADNLDIVQNMF
                     LGRERGKSWLLDEADMEQAARRTLASLSVRTVTSVRMPVAALSGGQRQTVAIAKAVLW
                     DSKVVLLDEPTAALGVAQTRQVLDLIRRLAEQGLGVVLISHNMADVFEISDRIATLYL
                     GRMVAQVPTKDISNTQAVELITAGRSGDLGLQRPESVTV"
     gene            complement(9419..10546)
                     /locus_tag="VFE39_12930"
     CDS             complement(9419..10546)
                     /locus_tag="VFE39_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6259548.1"
                     /translation="MRIRRAALAAAGLGLALTLAACGQNAAAPAAPPASSAPAGGVKV
                     GVILPETASSARWEGFDKPLLTKAMQAQGLDADIQNAQGDEQKFSTLADGMISSGVKV
                     LVIASISSDSGAAVAAKAKAQGIPVIDYDRLNLGGTSDYYVSFDNTKVGELQGQGLVQ
                     GIGNKPGAQVIEIEGAPTDNNATLFHNGQENILKPLYDKGTLKLVQSKPIDNWDNQQG
                     GTDFEQILTGNGGKVDGVVAANDGLAGAIITVLTKYGLNGKVPVTGQDATPDGLQAIL
                     RGDQYMTVYKPIDQEAEATAKLAAALAKGDTAAADAIATQTEKDPKGNRDVKSVLLTP
                     QLITKDNVKTVVDAGAVKASEICVAATQAACNQLGIKSGTN"
     gene            complement(11016..11330)
                     /locus_tag="VFE39_12935"
     CDS             complement(11016..11330)
                     /locus_tag="VFE39_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HET6259549.1"
                     /translation="MTDVSPPKLHPSCVTDLNQPVFDEEWQRRAFGLAVALSEFGHYP
                     WADFQRELINAIGTWEDAPTEARGRWEYYEHWVTALGAVVREHGMLEDGYVNPEDRDP
                     HP"
     gene            complement(11336..11962)
                     /gene="nthA"
                     /locus_tag="VFE39_12940"
     CDS             complement(11336..11962)
                     /gene="nthA"
                     /locus_tag="VFE39_12940"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HET6259550.1"
                     /translation="MTAQFGYPSDREETSAAQVRALEALLIEKGVITNGTVDKVLGYF
                     ESEMTPLNGRKIVARAWTDPEFARRLAADTPAAVAELDLPDGMAGAEGEHLAAVVDEP
                     GIHNLVICSLCSCFPWPVLGLPPYWYKDPVFRARAAREPRAVLKELGVPLDDDTEVRV
                     WDSSGHSRWFVVPQRPAGTEGMSEAELADLVTTESMIGVALADSPAGR"
BASE COUNT         1763 a         4321 c         4256 g         1691 t
ORIGIN      
        1 ttcccacacc atcgcgctgc gcgcgaacgt cgcggggtgc gcggctctgc tgggccgctg
       61 gctggaggcc gctgccgggt tcgagcgcgc cgccgccgat tccgccgccg tgctgggcca
      121 tcagcacccc gataccgtcg ccctccagga cgacctgagg aacgtgcatg atcccgctta
      181 tttcgcgatc cccgcaggag tatcggtctg atctccgcag caccgggttt gcccgacatt
      241 catcaggaac ggtcgcgcaa tacgccgagg agctcctcct ccaccgccgc gaaccgcgga
      301 gacgtgatca ttgcgagctc ccgggggcgc ggcagattca cggtcacctc gcggcggacg
      361 cgcgtgggcc gcggcccgag cacgaccacg cggtcggaga ggaacaccgc ctcccggatg
      421 tcgtgggtga tcagcagcac cgtccagtcg aagtgggcac gtacgccgtc gagccaggtc
      481 tgcatgtccg tgcgggtgag cgagtccagt gccccgaacg gctcgtcgag cagcagcacc
      541 gcgcggtcct gcaccaccgt gcgcagcagg gcggcccgct gccgcatgcc gccggacagc
      601 tcgtacgggt gggccgactc gaacccggtg aggccgaact cggcgaacag cggccgggct
      661 cgctcccggg ccgcccgccg cgacatcccg gccacctcga ggcccagcgt ggtgttgtcc
      721 agcacggtcc gccacggaaa cagcaggtcc tgctggggca tgtacgcgaa cggctcgacg
      781 tgccccgtgg cgtccacgcc gtcgacaagc acccggcccg agtccgggcg ctccaggccc
      841 gcgatgacgt tgaacagcgt ggacttgccg caaccactcg gtccgatcac ggagacgaac
      901 tcgccgggcg ccacgtcgaa ggtgacgccg tcgagcaccg gcagcgggcc gggataggcc
      961 ttgcgcaggt cgtcgagcac cagcttggtc tcgtgcccgg tccggtcgtg cctggcggcg
     1021 ctctcacgca cggcggcgct ccttcccgtg ccacgggatc accagccgct gcacgaggta
     1081 ggtcagtccg aatagcgtga ccgacacgag cgcagtgacc actacggccg ccagcaccag
     1141 atcggtccgg aaagagttct tctgcaggct catgaagatc ccgagtccgg ccgtcgcgcc
     1201 gacgtactcc gcgaagatcg caccggtgac cgcataggtg atgccgatgc gcagcgcggt
     1261 gaagaacgac ggcatcgctc cgggcacgcg cacatagcgg aactgcttcc agcgggacgc
     1321 gcccatgctg cgcagcagat tggtcgcctg ccgctcggtg gcggcgaacc cctcgatcaa
     1381 cccgacggcg atcgggaaga acgtcaccag cgcgatcacc agcaccttcg gcagcagacc
     1441 gaagccgaac cagatgatca gcagcggggc gatcgcgatg atcggcagcg tctgggacgc
     1501 cacgaggagg ggcgtgaacg cgcggcgcag ccacggggag aagtccaccg cgatggccag
     1561 cgcccagccc agcgccagcg acaccccgaa cccgaccgcc gtgacctgca gagtcgggag
     1621 ggtgttcgcc cagagctggt cccggaacgc ccagccctgc gtggccacgc gcagcgggga
     1681 gggcagtatc tccggccgga cgtcggctgc cgtgacgtac cactgccagg ccacgagccc
     1741 gagcgcgacg aggacgagtg acggcacgac cgccgagacc gtgcgtcgcc agcccgtcct
     1801 cgttgcgctc acggcttcgc caggtactgg tcggtgaacc aggtcgagaa gtcgggccgc
     1861 tgggtgagtg gcttcccgtc cgggccggtc agcaccccgg agtcgaagac ccaccccgag
     1921 aagcccgacc acttctccag cgtctgctcg ccgaccttgc cggaggcgac gcggaggtac
     1981 tggtcactga gcatcagctg gctgcgcatg acgagctccg gctcggtgaa gacgccgggg
     2041 ttggcgtcca tcagcagctg ggcggccttg gcggggtcgt cggcgccgag ctggtagccg
     2101 cgctgcgctg cctgcacgaa cttcgcggcc aggtccggat gggctttcag ccacgggctg
     2161 ttgccgatca gcagcacgtt gtaggcgtcg gggaagccgt agtcggtgta ggcgaaagtc
     2221 ttgaggggct gtccgtgcag gtcggcctcg atgccctccc acgccatgaa cggttcggtg
     2281 aagtcgacct gtcccgcgta cagcgcctcg taggcggacg tgccgagcac cacggtcttg
     2341 aagtcgccct tgccccccgc gtcgcggatg acggcctgca tcttctgctg ctcccccggc
     2401 ccaccgaacc cgccataggt cttgccgtcg aggtccttcg gggagttgat gtcggtgcgg
     2461 tcggcgcgca ccgcgatccg cgtggcccag tgctgcagca ccgccatcac cgacgtgatg
     2521 tccgcgcccg cagccttcga gaaggtgaac gagtcctgga agctgatgcc gaagtccgcg
     2581 gcgcccgacg agacgagggt gtcgggcgag gtgttgttgt acgggaggaa ctgcacgttc
     2641 agcccggcgt cgcggaacca tccctcctgc tgggcaacgt agaggccggt gtggttggtg
     2701 ttgggcgtcc agtccagggc gacgcggatc gtctgcgggc cgccggcggc gtctccaccg
     2761 cctgcggccc cgcgaccggc gcacccggcg agcacgaccg ccagcacggc gaccaggacg
     2821 gtgacgatcc gagcggtccc gagacgggcg cgcagcacat ggggtgtacg gggagaaggc
     2881 gggcgtacgc cgctcacgcg ggttcctccc tacgccggta ctaaccgggt caggtgcgaa
     2941 cggtcggcgg ccggtcctgc cgccctctca gcctcggaac gcgggctccc gcgggtcgcg
     3001 tagagaatac gcgtcgcccg cgaacggtgc agcgccccgg ggtcacccgt cgcgatcgcg
     3061 cggagcccga cgaacagccg cacccgcacc accgcggccc gctgccctgt gccgtcaagc
     3121 ccgtggccgg tgtcgctttc gccagccctg gcccgagatc ctgtcggttc ctccctcgac
     3181 gctgcgcgcc ccactcacgt tgggtgagga catgggtgca tcaacggcaa gaaagtgcgt
     3241 ggaggtcccg gccggcaccg gccgggccgt gcacgtgacc gcgggcgagc ggatccgggt
     3301 ggtcgatccg gcgggcggcc aggtcggcga cgtcttcgcg ttcgccgggg ccgagcacca
     3361 cagcgcggcg cacacccggg ccgccacgag caggctgttc cccgccgtcg gcgagcactt
     3421 cgtgaccgac cggcggcgcc cgatcctgac gttggtcgag gacacctcgc cggggcagca
     3481 cgacatgctg atcgccgcgt gcgaccccga gcggtacgcg gcactcggag cccccgggca
     3541 ccggtcgtgc gcggagaacc tgagggagac ccttgccgcc gtcgggcacg actacacggg
     3601 cccgaccccg cagccgatca acgtcttcat gcggatcccg gtcgacggcg acgggcggct
     3661 gcgctggctg tccgcgccga cggcgcccgg cgacgcgatc accttcgagg cggtgctgga
     3721 ctgtgtcgtc gtggtctccg cctgcccgca ggacatcgtc gggatcaacg caggcggcct
     3781 gtcgccgctc gctatcgaga tcctgccccg caacccgtcc tgagccgacc gccgggttcg
     3841 agcggctacc ccgacgaaac ggcgtcggcc tccagcgcca ggaacaggtc gacgcggtcg
     3901 gcggtgcgcc ccacgtcgcg gccggtgagc tcggagatgc ggttcagccg gttccgcaac
     3961 gtgttcacgt ggacgtacat cgcggccgcg gtggtcgccc actgcccgtc gtggccgagg
     4021 aacgcgcgca gcgtcgcttc gagctcgccg ccgcgcttcc tgtcgtgggc gcgcatcggg
     4081 ccgagcacgc tgtcggcgaa ggcctgcagc tgctcggtct cctgcagcgc gagcagcagc
     4141 tggtaggtcc ccagctcggc gaacgtcgcc acgtcggctc cgccgtgcct gtcgcgcagc
     4201 acccggcagg cctcccgcga ctgcaccagc ggttgccgca gcttcgccgc cgacgcggcc
     4261 gggtcgctga gcccgacaac gggccgctcc ccggggaacc ggcgggcgac cgcctcggcc
     4321 aggcgcccag cgaccgcggc caccccgtgg gcgggcccgc gccacggcag cacggccacc
     4381 acgtccgtgc ttccgcccgc cactacggcc ggcaggcctt ccgcgaggaa gaaatcggtg
     4441 acggcctcgg caacgcccgg cagggtcggc gggtcggcat gggcctccgc ggccgctgac
     4501 cccgccgctc cggaccgctc cggaccacgg cggcggcgga aggcgacggc gcagacggcg
     4561 aggggcgcct ccggatcgac accgaaggcg cgcagccggg ccggcacctc agccgcccgc
     4621 tgggctccgg agaggatcat gtccagcagc tcgccggcga agcgctgctc gatcgcctgc
     4681 acggtctgct gtctcgccac ctccaggctg agaaaccggg ccgcctggtc cagcgcgtcg
     4741 cgctcggcgc ggtcgagcgc gaccgcggga cgcaggcaca gcagggccgc gtccgcgtcg
     4801 ccgacggcgc cgacagggaa gagcgcggcc gggccgcggg ggccgaggtc gacctccagg
     4861 ggcgtcggcc gccgggccag tgcgtccgcc gcgatccgca gctcgccctc gtcgagccgg
     4921 cccgcggccg cacccagcag ccggcccatc cggtccacca ccgccagcgg gaggtcgtgg
     4981 tcgcgcctga ggatgtccag cacgccggac gcccccgcgc cgtgcgagat cgccgccgcc
     5041 agcgcgttcc cccggcgcac catgccaacg agcgcgtcct gccggtgctc ggtgtagatc
     5101 gccgcggccg cacgcgtgac cgccgtgaac gggacgtcga tggcgatctc gaccagcggc
     5161 agctcggcgg agcggcaggc gtccacgagc gcctgcggag tggtggtgca ctgggcgcgc
     5221 agcccgaagc ccattccggc ggcaccggct cggaccacac cggccacgaa atccgccggg
     5281 tcggtcccgt cgagccacag cccgttcgtc aggacgagct cccgctcccg caggtagggg
     5341 gacgggtcgg gcagctcggt gatgtgcacc cacaggacct cgcctgccag ggcgccggac
     5401 cggcccggca cgaggacccg cagcgcgagc gccgagtcgt ccagcagtgc accaaggctc
     5461 agcatgctgt gaacgtatcc agctgtggcg cagcaaccta gaggttccgc cagggaccgg
     5521 gcacagcgcc ggccgcggtc gtcaccatcc gcggggtaca tgggcgtcga gcgcccggga
     5581 ccgaagaggg gccgggtcac ggactgctgc acgcccggcg gttccccggc ccgaacggat
     5641 ttccccggag cggtgaaccg taacgccgtc gtcgctctgc gcgagggttc tcctactgga
     5701 ggtgaccgtt atgacgacag caatgtggct gatcctggcg gtaggggtgt tcatcggtat
     5761 gtacgtcggt cgttggtggg ccgagatcag gcgggccaac tacgacatgg accggatctg
     5821 ggccatgcgc cgcaactacc gcgactgatc ccggcccgag gccgctcaga ccgcgccgag
     5881 gatcggctcg aaggccagct cggccgctcc gaccagcttg gcgtcgcggc cgaacgggct
     5941 cgtctcgatc cgggtgcccc cgaccgcccg gctcaccagg ctccgctcgt gcaccttcgc
     6001 acgcacctcc ccgaccacgg caggtgggag tgcggtgaac aggtcgccca gcaccaccag
     6061 ctccggcgcc atcatgttca ccacggtgac gagcccggtg gcgagccact ccgtgaactc
     6121 gtccagcggt ccggggagcg gggcagtggc cagcgatcgc agctcggcca ccaccacccc
     6181 gcgcggggtg tcttccggga gcccgagcgc gcggcacagt gccgcctccc ccacctccgt
     6241 ctcccagcag cccctggatc cgcagtagca gggcctgcca cctggccgga cgacgaggtg
     6301 accgagctcg ccgacgtaac cccgggtgcc acgcaggggg cggccgtcgg agatcacgcc
     6361 gccgcccacg cccttgtccg ctgagatgta gaccaggtcg tcgacgttgc gggcgtcccc
     6421 gcggacgtgc tcggcgagcg cgccgagctc ggcgtcgttc gccacctgaa cggggacctt
     6481 cagcacggtg accagccggg cgccgagcgc cacctcgcgc caggcgagat tgggcgcctc
     6541 gtgcacgaag ccgtcgtcac gccggaccac gcccggcacc gacaccccca cccccagtgc
     6601 tgtcacggcc agctcctcgg cgagcagctg tgccgactcc gcgacgtggg tgatcacctc
     6661 gcccggcgtg cgggtgcggt ggtgcaggtt ccagctgtgc cggccgagca cctgcccgcc
     6721 gataccgacc atcgacatcg ccacctgctc gacacggacg tcgacggcga gcaccacggc
     6781 cgcctccggc ttgggcagca ccatcagcga tggccgaccg gcgcctttgc gctgcgccgg
     6841 cagcgcctcg gtgacgacgc cggcctctgc gaggccgtcg accaccgcct tgatcgtgct
     6901 gcggttcaga cccagctcgg tggccagggt ggcccgcgtg cacggaccgt caacgtgcag
     6961 tcgtcgcagc agcgctgctc ggttgtggcg tctggcctcg tccggccggg cgcccgaggt
     7021 ggcgccggtc gcgccgccgc caccgtcacc accccaggtg cggttcacct gacggcgacc
     7081 gccgagcgcc gccgcgacag cacgtccacc cctgccgcca ggagcagcac gatgccgttg
     7141 acgaggaaca cgacggacgc gggctgcccg agcaggccga ggccgttgtt caccgtggcc
     7201 agcaccgcac cgccgatcac ggcgttgctg atccgcccgc ggccgccgaa cagcgatgtg
     7261 ccgccgatga ccgcaccacc caccgcgaac agcagcgtgt tgccaccacc cgcgctcggc
     7321 gacaccgagc ccaccttcga ggagtagacg atggcgccga ccgccgcgaa cgccgtggag
     7381 atcacgaaca cggcggcccg gatgcggacc acgtcgatac cggcgcggcg cgcggcctcc
     7441 cggttgccgc cgaccgcata gacgtgcctg ccgaagcggg tgcggtcgag gacgtaggtg
     7501 ccggcgacga gcagcacgag cacgagcggc acaacgtagg gcacgccgga gatctgcagc
     7561 tggcccgggg agcggttgac cgtgagcagg taggtcgcca cggccgcgag cacgaccacc
     7621 gcagcggtct tgatcaggac gaggcccgtc ggttgggcga cgagcccgag cctgcgccgg
     7681 gcaacctggc ggttgatcat caccgccgca tagccgccgg cagccacgat gaacaggacc
     7741 cagctgccca gcgtcgagag gttgccgttg gcgaccgcgt tgatcacggg gtcgcgcagg
     7801 cccagcgtgc cgccgtcgcc gatgagctgc aggatcacgc cctgccaggc gatgaacaac
     7861 gcaagcgtga cgacgaacga cggcatgccg accttcgcca ccaggaagcc cgtgaggcag
     7921 ccgatcacca cgccgacgca cacggcgagc agcatctcga gccacgggtt cggcgcgaac
     7981 ccgacgacca ggagcaggag tgccacggcc gcggccgcgg cacccgccca gatccgcagc
     8041 agcaccgcca gcacgatcgc gacgacgatg accgcggcga acagcaggaa gacgggggtg
     8101 cccatcgcgc cgagcaggtt gccgctgctg accaggtgca gcgccatcac cgatgccgtg
     8161 agcccggacg ccgtgcccgc cgccaggtcg atctcgccgg tgagcaagac gaacaccagc
     8221 cccatcgcga tgatcgtctg cccggcgccc tgctggagca ggttggccag gttgagcagg
     8281 ctggggaacg tgtccggcgc gagcgccgtg aacaggatga acagcgccag caggccgagc
     8341 agcgccggca gcgcgccgag gtcgccgctg cgcaggctgg tgaggtagcc acggagcgcc
     8401 tcccccgtgg tcctggcggt cgtgtcgatc ccgaagtcgg cagcgcgcgc cgatgcgggc
     8461 gctgcccgct cggccgcacc ggtcccggcg gccggggtag tgctccccgg ctccgccttc
     8521 tgctccgtct gggtgctctg ggggccctcg gtgggtgccg tcatcagacc gtcacgcttt
     8581 cgggtcgctg caggccgaga tcaccggaac ggcccgcggt gatcagctcg acggcctgcg
     8641 tgttgctgat gtccttggtg ggcacctggg cgaccatccg gcccaggtac agggtggcga
     8701 tgcggtcgga gatttcgaag acgtccgcca tgttgtggct gatcagcacg acgccgaggc
     8761 cctgctcggc gagtcgcctg atgaggtcga ggacctgacg ggtctgggcg acccccagcg
     8821 cggcggtcgg ctcgtcgagc aggacgacct tcgagtccca gagcacggcc ttggcgatgg
     8881 cgacggtctg ccgctgtccg ccggagagcg ccgcgaccgg catccgcacg gatgtcaccg
     8941 tgcgcacgga cagcgacgcg agggtgcgcc gggcggcctg ctccatgtcg gcctcgtcga
     9001 ggagccacga cttgccgcgc tcccggccga ggaacatgtt ctggacgatg tcgaggttgt
     9061 ccgccagcgc gaggtcctgg tagacgacct cgatgccgag cttcgcggcg tcggtgggag
     9121 cgttcatcgt caccggcctg ccctcgaaca gcacctcgcc gctgtcgatc gggtggatcc
     9181 ccgcgacgca cttcaccaac gtcgacttgc cggcgccgtt gtccccgacg agcgcggtga
     9241 cctcgcctgc gcggacggtg agatccacgt cgtgcagcac ctggacggcg ccgaagctct
     9301 tgttcacccc gcgcaactgc aggatcgggt cggtcattcg gacacctccg gtgcccgtgg
     9361 gggatacgag tgtcagaggg gcccactcgt atccgcccac gagggtcgag tcgtcgagtt
     9421 agttggtgcc ggacttgatg ccgagctggt tgcaggcggc ctgggtggcc gcgacgcaga
     9481 tctcggaggc cttgacggca cccgcgtcga ccacggtctt cacgttgtcc ttcgtgatga
     9541 gctgcggcgt gagcagcacc gacttgacgt cgcggttgcc cttcgggtcc ttctcggtct
     9601 gggtcgcgat cgcgtcggca gcggccgtgt cgcccttcgc gagcgcggcg gcgagcttgg
     9661 cggtggcctc ggcctcctgg tcgatcggct tgtagaccgt catgtactgg tcgccacgca
     9721 ggatcgcctg cagaccgtcg ggggtggcgt cctggccggt caccggcacc ttgccgttga
     9781 ggccgtactt ggtgaggacg gtgatgatcg cgccggcgag accgtcgttg gccgcgacca
     9841 cgccgtcgac cttgccgccg ttgcccgtca ggatctgctc gaagtcggtg ccgccctgct
     9901 ggttgtccca gttgtcgatg ggcttcgact gcaccagctt gagggtgccc ttgtcgtaca
     9961 gcggcttgag gatgttctcc tggccgttgt ggaacagcgt cgcgttgttg tcggtcgggg
    10021 cgccctcgat ctcgatcacc tgcgcgcccg gcttgttccc gatgccctgg acgagaccct
    10081 ggccctgcag ctcgccgacc ttggtgttgt cgaacgagac gtagtagtcg ctggtgccgc
    10141 cgaggttgag gcggtcgtag tcgatcacgg ggatgccctg cgccttggcc ttcgcggcca
    10201 cagcggcgcc ggagtcgctg ctgatcgagg cgatgaccag caccttgacg ccgctggaga
    10261 tcatgccgtc ggcgagggtg gagaacttct gctcgtcgcc ctgggcgttc tggatgtcgg
    10321 cgtcgaggcc ctgcgcctgc atcgccttcg tgagcagcgg cttgtcgaag ccctcccaac
    10381 gggccgagct ggccgtctcc gggaggatca ccccgacctt gaccccgccg gccggagcgg
    10441 agctcgcggg cggcgcggcc ggtgccgcgg cgttctggcc acaggcggcg agggtgagcg
    10501 cgaggcccag ccccgcggcc gcgagcgcgg ctctcctgat gcgcattgtc gtccttccac
    10561 agcgtcgggg accccgcggt tgcgggtaaa tgttgtgacg gacaacgtat gtcccccagg
    10621 cgagtggcgc caagcacact gaatcgttca agttgtcact gagaggtcac gattctgtga
    10681 cgctcacccg gatttgttgc gagcgtcacc atgagggcga aagagcgatg aaggcgcgtc
    10741 atgccctttg ggacgcgctg ttggcccccg ccgtcccctg cacagtgacc acgccatcgg
    10801 gttcacgacg aggagagcca cgacgacgga gtcgagccgg tcaccagccc acccgcccgc
    10861 ggcgcgccgc tcgtcgaccg ggtggtctcc gggctcctgc ggcgaccgga ggagacgggt
    10921 acagggtgac ccgcgggcta cgcgtcccgc tgttcgacgc gggctggcca cgctcggccg
    10981 tcctacctcg ggtcacccga tgggctggcg gcgcgctaag gatgcgggtc ccggtcctcg
    11041 ggattgacgt agccgtcctc gagcatgccg tgctcgcgta cgacggcccc gagcgccgtg
    11101 acccagtgct cgtagtactc ccaccggccg cgcgcttcgg tgggggcatc ctcccaggtc
    11161 ccgatggcgt tgatcagctc ccgctggaag tcggcccacg gatagtggcc gaactccgac
    11221 agggcgaccg ccagaccgaa ggcgcgccgc tgccactcct cgtcgaagac gggctggttg
    11281 aggtcggtga cgcaggacgg gtgcagcttc ggggggctca cgtcggtcat cccgctcacc
    11341 gcccggccgg ggagtcggcg agcgcgacgc cgatcatcga ctcggtggtc accaggtccg
    11401 cgagctcggc ctcggacatc ccctccgtgc ctgccggccg ctgcgggacg acgaaccagc
    11461 gggagtggcc gctggagtcc cacacgcgta cctcggtgtc gtcgtccagc ggcacgccga
    11521 gctccttcag gaccgcgcgc ggctcgcggg ccgcgcgcgc ccggaagacc ggatccttgt
    11581 accagtacgg cggtagcccg agcaccggcc aagggaagca cgagcagagc gagcagatca
    11641 cgaggttgtg gatcccgggc tcgtcgacga cggcggcgag gtgttcaccc tcggcgcccg
    11701 ccataccgtc gggcaggtcg agctcggcga cggcagcggg ggtgtcggcg gcgagccggc
    11761 gggcgaactc cgggtcggtc caggcccggg ccacgatctt cctgccgttg agcggggtca
    11821 tctccgactc gaagtagccg agcaccttgt cgacggtgcc gttcgtgatg acgcccttct
    11881 cgatgaggag cgcctccagc gcgcgcacct gcgcagcgct ggtctcctcg cggtcgctcg
    11941 ggtagccgaa ctgcgcggtc actggtgtcc ctcctggtcg actggggcga ggtagacctc
    12001 gtagagctcg gcgtagagcg cgccggcgcc c
//
LOCUS       DASRSC010000263        15742 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4685238,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000263 DASRSC010000000
VERSION     DASRSC010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 15742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15742
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4685238"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VFE39_12945"
     CDS             complement(<1..616)
                     /locus_tag="VFE39_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HET6259551.1"
                     /translation="MRASRLVTLLFTLQRLRGATAAELARELEVSERTIYRDVAALSA
                     AGVPLFTEPGRGGGIRLVDGWRTRLDGLTAQEAAALFAVGAPQVLAELGMSAALAGAQ
                     AKLLATLPAELREHARTVAERFHLDAPGWFHTPQQVTQLAAVAEAVWDQQQLRISYRR
                     RADHVKRTIDPLGLVLKAGTWYLAARSVSGGCIDTARSVSGGCID"
     gene            701..1189
                     /locus_tag="VFE39_12950"
     CDS             701..1189
                     /locus_tag="VFE39_12950"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6259552.1"
                     /translation="MTVREDAVRRPDGSNGIYGVVDKPTFALVIPRDGDQLHMVEQFR
                     YPVGRRRWEFPAGTAPGRAEIDPADLAAQELREETGLQAGQWELIGRLDVAPGMSSQT
                     GTIWLATDLTEGPTKREHTEQDMRAAWFTSAEFEEMARAGEITDSQSLAAYLILQLHD
                     RR"
     gene            1277..2440
                     /locus_tag="VFE39_12955"
     CDS             1277..2440
                     /locus_tag="VFE39_12955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005463822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6259553.1"
                     /translation="MDLRETGEQQLLRSSVAAMAAKYGHDYLIDKARTGGHTTELWAD
                     AAAAGYLGVAVPAEYGGGGAGMVELSIVAEEVAAAGCPLLLIVVSPAIAASVIARSGT
                     TDQKERWLPRFADGSVKMSFAITEPDAGSNSHRITTTAHRDPDGGGWRLRGTKYYISG
                     VDETDATLVVARLEDAASGTLRPAMFIVPTDTPGMRYQPIDMVIHSPEKQFTIYFDDA
                     LVPDDALVGGEEAGLAALFAGLNPERITVAAYTNGLARYALRKGVDYAKDRTVWQAPI
                     GSHQAIAHPLAKAHVEVELARLATTRAAWLYDQGDQAAAGEAANIAKYAAAEAAINAV
                     DAAVQAHGGNGMSVEYGVGTLIGAVRAARIAPVSREMILNYVAQHSLGLPRSY"
     gene            complement(2494..3474)
                     /locus_tag="VFE39_12960"
     CDS             complement(2494..3474)
                     /locus_tag="VFE39_12960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004558400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene cyclase"
                     /protein_id="HET6259554.1"
                     /translation="MTALDWMLDSEPSIRWQALRDLADASAEVVAAERARVATEGWGA
                     RLLALQGDDGQWEGGAYFPARSDDSVDNDLSDDGDHSDDEGQPWTATAYSLLLLRDLG
                     VEPRTDQVCRAVALVRDHCRWEEGGQPFFTGEVEPCINGMTVALGAYFDQDVDGVVAR
                     LLGEQLEDGGWNCWAEHGSVRSSFATTINVLEGLLARERASGGSAESIAARRRGEEYL
                     LERKLFRRKSTGEVVDPAWLQFSFPTRWHYDVLRALEYFRAAGDPPDQRVDEAIHLLR
                     SKQQPDGTWLLENTHPGDVHFTLEDGDGRPSRWNTLRALRVLSWYGQSAG"
     gene            complement(3775..4626)
                     /locus_tag="VFE39_12965"
     CDS             complement(3775..4626)
                     /locus_tag="VFE39_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6259555.1"
                     /translation="MDGVELCLETFGDRVDPALLLISGMAASMDWWDVGFCERLAGEG
                     RFVVRYDHRDTGRSVASPVGKPSYSSDDLATDPLRILDALGIRRAHVAGISMGGGIAQ
                     HLAARHRDRLFTITLIATSPAGARADQKGLPPIERRLAATFENPTPAPVWNDRAAVVD
                     YLVDGERPFAGSLGFDEDRVRRLANIVVDRTRDIAASMTNHWVLEGGSAARFRLADIE
                     VPTMVLHGTADPLFPFGHGEALAAEIPNASLVPLDGMGHEMPPPQLWDVVVPAIVAQT
                     SGDSRRS"
     gene            4902..5381
                     /locus_tag="VFE39_12970"
     CDS             4902..5381
                     /locus_tag="VFE39_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019808538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6259556.1"
                     /translation="MDADIGRLYAERGVAGVRPRFSMALIRLHHRGPMTVKELAGQID
                     VTHSAMSQTVTAMRREGLVETSTGSDARTRTVTLTDRGRALVPFLEAEWRATERAWAE
                     LEAELPYALTRVVDDITAALQRRSFLDRITQQLDRPPDGADEPRRASGDQSGREHRA"
     gene            5378..6634
                     /locus_tag="VFE39_12975"
     CDS             5378..6634
                     /locus_tag="VFE39_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005450599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259557.1"
                     /translation="MSVIDGIIDTRPLRSHPAFRRIWIGTTAASFSGQVAVVAVLYQV
                     WELTHSSAWVGAIGIATAVPTIIFGLLGGALADATDRRRLVAMTSIGAVTAAILLAVQ
                     AGLCLGSLPLVLVLVAAQTSCVALGASARRTFVPRLLPRNQVPAGIALNHVSFQAAML
                     VGPAVAGLVLAHGGLTWAYVLDATAITISLYGVARLPSMRPTGGATPASLASTWEGWR
                     FIVRRPALSGSLASDLAQTVLAMPIALFPVLNEQRFGGSPQTLGLFLSAIAVGGIAAS
                     LTSGILTRVRRPGMVSLVAAATWGLALAAFGLADTLFPTLAWLAVAGAADTISVISRG
                     TLIQLATPDPYLGRVSSVENIVGVGGPGIGNARAGAVASLTSASTAAVTGGLACAVVV
                     VALAATNPALRRWRHQYAEPVDQPSG"
     gene            complement(6707..7939)
                     /locus_tag="VFE39_12980"
     CDS             complement(6707..7939)
                     /locus_tag="VFE39_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6259558.1"
                     /translation="MTTTPSVEPDLMDPALIADPYGGYGRLREQGPVLRGRTMDGGQA
                     WYVTRQQDVRFVLGDPRFVNNPAAVPGVQVDNVRDKTLEKLGIPRELAHYLTESILDA
                     DGADHTRLRKLVSRAFTVRRVSELRPRVEAITAALLDGLPSTVDLIEHFAYPLPITVI
                     CELVGVPEVDRPAWREWGIALLSMNPEVIPGAVRDMVDHVRDLIRRRRAEPADDLLTA
                     LVHAQDEDGDRLSDDELVTMVLTLVMAGHETTAHLIGNGTVALLTHPDQLDLLRRDPS
                     LWPGAVHELMRFCGPVQITRLRYAAEDLDLGGVRVRAGDAVQAVLVSANFDPREYTDP
                     ERLDVTRRPTGRGEGHVGFGHGIHYCLGAALARQEGEVALCALFERFPDLALVNAQQE
                     WVQIPGSRRLAQLPVRLG"
     gene            complement(7975..8682)
                     /locus_tag="VFE39_12985"
     CDS             complement(7975..8682)
                     /locus_tag="VFE39_12985"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6259559.1"
                     /translation="MAAPEFANSRMERVLDTAAELLVRWGYQRVTIDEVARHAGIGKG
                     TVYLHFRTKDALFLTVLLRAHRQIVTAMADLMEAEPAHVLPSRLLRSVFLQLAADPVA
                     RALYLGDAEVLGRLAHEAEGTLGGLVERREEVLGEHLRLLREARCLRTDLEPDALLYL
                     FGAIGAGFFFLGGTPGSRPELDMETRADLLEHALAAAMQVPDPPTAELERLAPVVAEL
                     YRSLIDLIDHEWRRRVR"
     gene            8842..9615
                     /locus_tag="VFE39_12990"
     CDS             8842..9615
                     /locus_tag="VFE39_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6259560.1"
                     /translation="MADYEHLLVKHDGDTVRITMNRPERRNALSEGHLRELLVAFETA
                     GRSDATGIVLAAEGKAFSAGHDFADVAARDLAGVRDLLQLCTRVMRTVQEVPQVVIAR
                     VQAIATAAGCQLVASCDLAVAAESAAFALPGGRGGWFCHTPAVPVARSVGRKRLMEMA
                     LTGDAIDAATAAEWGLVNYAVPDAELDARVDELLARSTRGSRFGKGVGKQTLYAQLDR
                     PEADAYAIAVEVMASLSQSSGAREGMASFLEKRPPTWPD"
     gene            9703..10356
                     /locus_tag="VFE39_12995"
     CDS             9703..10356
                     /locus_tag="VFE39_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6259561.1"
                     /translation="MSSSTSRVPDSGPAADRAYDLTKRLVLSGEFPGGHLFSEGEIAG
                     LLGMSRTPVREAFLRLQAEELLRLIPKRGAVVVPVSPEEAEDVLDAREAIEIAAVRRL
                     LTRPDLVPAAVADLRAALRIQQAHADAGDVHAFAAADEDFHGRIVAAGRNGLTIRFYA
                     TLAERQQRMSMRALGPVPERLPGVLREHAELIATIENGDEQGFGTALRAHLDGIHRS"
     gene            10353..11597
                     /locus_tag="VFE39_13000"
     CDS             10353..11597
                     /locus_tag="VFE39_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259562.1"
                     /translation="MTAHTPVHQPAAGRATVQATGRRAWRPVVIAMFCCGWGGNQFTP
                     LLILYREAGYSVITVDALLGTYVVGLVPALLLTGGLSNRYGRKPVMLAGTAVALLASV
                     LLALGEHGTIWIIAGRLLAGAGVGVAMAVGSTWVKELTDADPASSPGLGTRRPALWLT
                     LGLAAGPGVAGVLAQWAPWPMVLPYVVHIVITAVALPFLLRSPETSGGPVTARGRRYT
                     GIRHPRFRRVITPMAPWIFGSASVAYAIMPQLVDSRLGSWGLAYSTLLVVCTLGAGVA
                     VQPLAKRLDRTTSARGVVVGMIVMSVGLGLSAVAAALRMPLLVLAVAIVLGAAYGIAV
                     VSGLLELQRLARPEELAELTGVYYALAYIGFLFPSLLALLSSLATYTALLCGLAVIAL
                     AGTTVIVRHSRSHLPLKSGVGA"
     gene            complement(11594..12358)
                     /locus_tag="VFE39_13005"
     CDS             complement(11594..12358)
                     /locus_tag="VFE39_13005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET6259563.1"
                     /translation="MIVFDGVGKRFGDNVVLSDLDFTVAPGEHVTLIGPSGSGKTTIL
                     RLLMCLESVNAGTIRVAGRYLSHMEKGGTLVPADEKHSREVRKRIGMVFQQFNLFPNM
                     TVRGNLIEAPTRVLGLSKDEANARAEELLDLVGLRERIDEHPSRLSGGQQQRVAIARA
                     LAMRPDVLLLDEVTSALDPELVAGVLALLRDIGETTDITILCVTHEMGFARDFSHRVM
                     MFDGGQVIEDAPPERLFSEPEHPRTKEFLKAVLSRG"
     gene            complement(12363..13019)
                     /gene="ehuD"
                     /locus_tag="VFE39_13010"
     CDS             complement(12363..13019)
                     /gene="ehuD"
                     /locus_tag="VFE39_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuD"
                     /protein_id="HET6259564.1"
                     /translation="MTQVWDWNYAFAILPSLLEGLGLTVLATVLGSVVAMILGLLLAV
                     LRQGPPLLSVAVRGFVEFVRSTPLLVQIFFLYFVLPQFGIVLSALTVGVIALGVHYAC
                     YASEVYRAGIEAVPRGQWEAATALSLPRSRVWTGVVLPQAIPRVVPALGNYVISMFKE
                     VPLLTAIGLLDVVGRAQQAGDTYFRYVEPYTIAGLLFLLLSYPASLLVRRLERRVGTI
                     "
     gene            complement(13016..13723)
                     /gene="ehuC"
                     /locus_tag="VFE39_13015"
     CDS             complement(13016..13723)
                     /gene="ehuC"
                     /locus_tag="VFE39_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter permease
                     subunit EhuC"
                     /protein_id="HET6259565.1"
                     /translation="MDNLGTFLSVLAQGLPETLIATVGGIVLTILLSFAAGLALLSPS
                     RPVRVISRIYVEGLRGTSEVVQLFWLFFALPVLVGFEIVPIWGGVLVLGLNHGAYGAE
                     IVRGAIQSVPRAQYEGALALSLSPAQRMFRVILPQAVVEMIPPFNNLFIQLLKSTSIL
                     SFVFISEITRQATQILVPRFNPQVLQIFLLLLLVYLVLSLLITAGMRALERVASARLG
                     RGPSPRAKRLASTGGRG"
     gene            complement(13731..14627)
                     /gene="ehuB"
                     /locus_tag="VFE39_13020"
     CDS             complement(13731..14627)
                     /gene="ehuB"
                     /locus_tag="VFE39_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0033286 - ATPase-coupled ectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033288 - ATPase-coupled hydroxyectoine
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0033294 - ectoine binding [Evidence IEA];
                     GO_function: GO:0033295 - hydroxyectoine binding [Evidence
                     IEA];
                     GO_process: GO:0033308 - hydroxyectoine transport
                     [Evidence IEA];
                     GO_process: GO:0051470 - ectoine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine/hydroxyectoine ABC transporter
                     substrate-binding protein EhuB"
                     /protein_id="HET6259566.1"
                     /translation="MTQRSWSRRDFLRRTAAGAVALGGITALSACTSTSSGGGSVLEN
                     ARNSGSMRIGIAGEEPYGFTGTDGRVTGEAPEVARAVLQGIGIRDVQAEQVDFGSLIP
                     ALNANRFDMVCAGMNITPQRCQQASFSVPDYTALTAFLVPKGNPQGVTTFQHIAEKKL
                     PLAVLGAAVEQGYAEAAGVPPGNISPFDDQNALLQAVTAGRVYAAALTDISLKWLAGK
                     NPQAGVEVTPGFTPTQNGREVVSAGGFVFRQADTDVRNAFNTELQTLHGNGRWVQIAS
                     PFGFSQSNLPTAGLTTEQLCAA"
     gene            14828..15727
                     /locus_tag="VFE39_13025"
     CDS             14828..15727
                     /locus_tag="VFE39_13025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259567.1"
                     /translation="MLRIAGLPVWVGPSEVISGARAVMGWTDEAVEVVAGLPERVSRL
                     LDDVDGLVARITTVADRVEAIADRADGIVGSVDEVLVGVRTAVARVDSVLADADGIVR
                     RVDPLLVEVRTTADSAATLVARAGGVAEGAAGLVARADGVAEGAAVLIARAGEVADGA
                     AELVAGAGRVADSAGGVIEKATAVADRAGTVVDQASGAADGATELLAVYQPIARQAAP
                     LARRFVEEFSEEEIRAAIQLVDHLPRFTEHMESDIMPILATLDRVGPDVHDLLDQLKE
                     MRQAIQGIPGLAFFRRRGEEREE"
BASE COUNT         2405 a         5629 c         5450 g         2258 t
ORIGIN      
        1 tatcgatgca ccctccgctg acgctccggg cggtatcgat gcaccctccg ctgacgctcc
       61 gggcggccag gtaccacgtc cctgccttca gcacgagccc gagcgggtcg atcgtccgct
      121 tcacgtggtc ggcgcgcctg cggtaggaga tgcgcaactg ctgctggtcc cacactgcct
      181 cggcgaccgc ggccagctgc gtcacctgct gcggcgtgtg gaaccagccg ggcgcatcga
      241 ggtggaaccg ctccgcgacg gtgcgggcgt gctcccgcag ctccgcgggc agggtggcga
      301 gcagcttggc ctgcgccccg gccagcgccg cgctcatccc cagctcggcg agcacctgcg
      361 gggcgccgac ggcgaagagc gcggccgcct cctgcgccgt cagcccgtcg agacgggtgc
      421 gccagccgtc gacgagccgg atgccgccgc cccggcccgg ctcggtgaac agcggcaccc
      481 cggccgcgga gagcgcagcg acgtcgcgat agatcgtccg ctcggacacc tccagctcgc
      541 gcgccagctc cgccgccgtc gccccgcgca gccgctggag ggtgaacagc agggtgacca
      601 gccgggacgc acgcaccccg gaatcgtgcc cgatccgggc acccggcagg gtgaccaggt
      661 ggagacgata agttcccgcc aggtctacgc caacagctgg atgacggtcc gcgaggacgc
      721 cgtgcgccgc cccgatggca gcaacggcat ctacggggtg gtcgacaagc ccacgttcgc
      781 cctcgtcatc ccgcgcgacg gcgatcagct gcacatggtc gagcagttcc gctacccggt
      841 gggtcggcgc cggtgggagt tcccggccgg caccgcgccc gggcgcgccg agatcgaccc
      901 cgccgacctc gccgcgcagg agctgcgcga ggagaccggg ctgcaggcgg gccagtggga
      961 actgatcggc cggctcgacg tggcaccggg gatgtccagc cagaccggca ccatctggct
     1021 ggccaccgac ctcaccgagg gcccgacgaa gcgcgagcac accgagcagg acatgcgcgc
     1081 cgcctggttc acctccgcgg agttcgagga gatggcgcgc gccggcgaga tcacggactc
     1141 acagagcctc gccgcctacc tgatcctgca gctgcacgac cggcgctgat caacggatcg
     1201 gttgactgtt ccttcctcct cccggcacgc tcgccacccg ccagcagtag cccgaccgac
     1261 ggaaagggtt cgtccgatgg acctgcgtga gaccggcgag cagcagctac tgcgatcctc
     1321 ggtggccgcg atggcggcga agtacggcca cgactacctg atcgacaagg cccgcacggg
     1381 cggccacacc accgagctgt gggccgacgc cgccgcggcc ggctacctcg gcgtggcggt
     1441 cccggccgag tacggcggcg gtggggcggg gatggtcgag ctctcgatcg tcgccgagga
     1501 ggtggcggcg gcgggctgcc cgttacttct catcgtcgtg tcgcccgcga tcgccgcctc
     1561 cgtgatcgcg aggtccggca ccaccgacca gaaggagcgc tggctccccc gcttcgctga
     1621 cggctcggtg aaaatgtcgt tcgcgatcac cgagccggac gccggctcca actcgcatcg
     1681 catcaccacc accgcacacc gcgatcccga cggcgggggt tggcgcctcc gcggcaccaa
     1741 gtactacatt tcgggggtcg acgagacgga cgcgacgctc gtcgtcgctc ggctcgagga
     1801 tgcggcctcc ggcaccctgc ggcccgcgat gttcatcgtt cccaccgaca caccgggaat
     1861 gcgttaccag ccgatcgaca tggtgatcca cagcccggag aagcagttca ccatctactt
     1921 cgacgatgcg ctggtgcccg acgacgcgct ggtgggcggc gaggaggcgg gcctcgcggc
     1981 cctgttcgcc gggctgaacc cggagcggat cacggtggcc gcctacacca acgggctcgc
     2041 gcgctacgcc ctgcgcaagg gcgtcgacta cgcgaaggat cgcacggtgt ggcaggcgcc
     2101 catcggcagc caccaggcga tcgcccaccc gctcgccaag gcccacgtcg aggtcgagct
     2161 cgcccggctc gcgaccaccc gcgccgcctg gctctacgac cagggcgacc aggcggccgc
     2221 gggcgaggca gcgaacatcg ccaaatacgc cgccgcggaa gccgcgatca acgccgtcga
     2281 cgccgcggtg caggcgcacg gcggcaacgg gatgtcggtc gagtacgggg tcggcacgct
     2341 gatcggcgca gtgcgcgcag cccgcatcgc ccccgtgagc cgggagatga tcctcaacta
     2401 cgtcgcccaa cattcgctcg gactgccccg ctcgtactag gaagtcgttt cataacgacc
     2461 tcctcagttg tcatggagct ggtcggcgga cagtcagcca gctgattgcc cgtaccagct
     2521 gaggacacgc agcgcccgta gcgtgttcca ccggctgggc cggccatcgc cgtcctcgag
     2581 tgtgaagtgg acatcgccgg gatgtgtgtt ctccagcagc cacgtgccgt cgggctgctg
     2641 tttggatcgg agcaaatgga tcgcctcgtc cacccgctgg tccgggggat ccccggccgc
     2701 ccggaagtac tccagggcac ggagcacgtc gtaatgccag cgggtcggaa acgagaattg
     2761 cagccaggcc gggtcgacca cttcgccggt gctcttacgc cggaagagct ttcgttcgag
     2821 gaggtactcc tcaccccgac gtcgagccgc gatggattcc gccgagccac cggatgcgcg
     2881 ttcgcgcgcc aacaaccctt ccagcacgtt gatcgtggtc gcgaacgacg aacgaaccga
     2941 accgtgctcc gcccagcagt tccacccgcc gtcctcgagc tgctcaccga gtagccgggc
     3001 gaccacgcca tcgacatctt gatcaaagta cgcaccgagc gcgacggtca taccgttgat
     3061 gcacggctcg acctcgccgg tgaagaacgg ctgcccgccc tcctcccaac ggcagtgatc
     3121 tctgaccagc gccacggccc ggcacacctg atcagtacgc ggttcgaccc cgaggtcacg
     3181 cagcagcagg agactgtacg cagtggccgt ccagggctgg ccctcgtcgt cggaatggtc
     3241 gccgtcatcg gacaggtcgt tgtcaacgga atcatcgctc cgcgcgggga agtacgcgcc
     3301 tccctcccac tggccgtcgt cgccctgcaa ggcgagcagc cgggcacccc acccctcggt
     3361 ggccacccgc gcccgctccg cagccacaac ctcagccgac gcgtcggcta gatcacgcaa
     3421 tgcctgccag cggatcgaag gctcggaatc gagcatccag tccaacgcgg tcatgggctg
     3481 agactagcta ggctccccgg acgagacggt cgtctagccg gacgtgtcac gaatccgaat
     3541 gacgctgatc tgtcacggat caagtggagc cgagccttat cgatgacaga tcagggcgca
     3601 ggcgaccaca cccgggcgcg gtacgcctca cgtccgctcc cgtgctcact cgcaacgctc
     3661 gctgtgcaag ctcgtctcca cgcagcgggg ctgcggagct cccggaagga atgaacagag
     3721 ggccggtcca aagtgtcccg gggtcacgcg tccgtcgacg ctcctggggc cgcgtcagga
     3781 gcggcgtgag tcgccggacg tctgcgccac aatcgccgga acgacgacgt cccacagctg
     3841 aggcgggggc atttcgtgac ccatgccgtc cagcggaacg agggaggcgt tcgggatctc
     3901 ggcggcgagc gcctcaccgt gaccgaacgg aaacagcgga tcagcggtgc cgtgcagaac
     3961 catcgtcggc acctcgatgt cggcgagccg gaaccgagcc gccgagccgc cctccaagac
     4021 ccagtggttc gtcatgctcg ccgcgatgtc gcgggtgcgg tcgaccacga tgttcgcgag
     4081 ccggcgcacc cgatcctcat cgaagccgag cgaaccggca aagggtcgct cgccgtcgac
     4141 gagatagtcg accacagccg cgcggtcgtt ccatacgggc gcgggagtgg ggttctcgaa
     4201 tgtcgcggcg agccgccgtt cgatcggtgg caggcccttc tgatcggccc gtgctcccgc
     4261 ggggctggtc gctatcaggg tgatggtgaa caatcggtca cggtgccgcg ccgccaggtg
     4321 ctgggcgatt ccaccaccca tggaaatgcc ggccacgtgg gcgcggcgga tgcccagagc
     4381 atcgaggatg cggagcgggt cggtcgcgag gtcgtcagag gagtacgacg gctttccgac
     4441 cggggaggcc acggagcggc cggtgtcgcg gtggtcgtac cgcaccacga aacggccctc
     4501 gcccgcgagc cgctcacaga aaccgacgtc ccaccaatcc atcgacgcgg ccatgccgct
     4561 gatcaggagc agcgccgggt ccacccggtc accgaacgtc tccaggcaca actccacacc
     4621 gtccatggtc acgagtcgct ccgccatgta cctgcagacc tgtcccaagc ccgagactca
     4681 tcggagacgc gcgctcgacc tgctccacga ggagatcgcg gacgtgctcg gggagccgac
     4741 cagatccgtt ccgcgctgga gcccgccccg gtgtcgcggc ccccttatat gagtgctgtt
     4801 atattagcgc tgtgacatct gccgacgacg gtgaccgatc cgccggcgac gacccgacgc
     4861 tggagagcgc gatcgggcca ctcaaggact tgctgtcgac gatggacgcc gacatcggcc
     4921 gcctctacgc cgagcgcggg gtcgccgggg tccgcccgcg gttcagcatg gcgttgatcc
     4981 ggttgcatca ccgagggccg atgacggtca aggaactcgc ggggcagatc gacgtcacgc
     5041 attccgcgat gagccagacc gtcacggcga tgcgccggga aggactggtc gagacctcga
     5101 ccggctcgga cgcccgcacc cgcaccgtca cgctgaccga ccgcggtcgc gctctggtgc
     5161 cgttcctcga ggccgagtgg cgcgcgacgg aacgcgcgtg ggcggagctg gaggcggagt
     5221 tgccctacgc cctcacacgt gtggtcgacg acatcaccgc agcgctgcag cgccgctcct
     5281 tcctggatcg catcacacag cagctcgatc ggccaccaga cggagcggac gagccgaggc
     5341 gcgcttccgg cgatcagagc gggcgggagc accgggcatg agcgtgatcg acggcatcat
     5401 cgacacgcgc ccgctgcggt cacacccggc gttccgccgg atctggatcg gcacgacggc
     5461 cgcgagtttc agcggccagg tcgcggtggt ggcggtgctc taccaggtgt gggagctcac
     5521 gcacagttcg gcctgggtcg gcgcgatcgg aatcgcgacc gccgtcccga cgatcatctt
     5581 cggactgctc ggtggcgcgc tggcggatgc caccgaccgc cgccggctcg tcgcgatgac
     5641 cagcatcgga gcagtgaccg cagcgatcct gctcgccgtg caagcgggac tctgtctggg
     5701 atcgttgccg ctcgtcctgg ttctggtggc agcgcagacg tcgtgcgtag ctctcggcgc
     5761 ctcggcccgc cgtaccttcg tgccccggtt gctcccccgc aatcaggttc ccgccggcat
     5821 cgccttgaac cacgtcagct tccaggccgc gatgctggtc gggccggcgg tggctggtct
     5881 ggtcctcgca cacgggggtc tgacctgggc ctacgtcctg gacgccacgg ccatcaccat
     5941 ctccctgtac ggggtggccc ggctgccgag catgcgaccg acggggggcg cgacgccggc
     6001 cagcctggcg tcgacctggg agggctggcg gttcatcgtc cgacggccgg cgttgagcgg
     6061 ctccctcgcg tccgacctcg cgcaaaccgt gctcgcgatg cccatcgcac tcttcccggt
     6121 gctcaacgaa caacgattcg gggggagccc gcagaccctg ggcctgttcc tgtcggcgat
     6181 agcggtcggc gggatcgccg cgagcctcac ctccggcatc ctgacccgcg tccgccggcc
     6241 gggaatggtc tcactcgtcg ctgctgccac ctgggggctg gcgctcgcag ctttcggcct
     6301 cgccgacacg ctgttcccga ccctcgcatg gctcgccgtt gccggagcag ccgacacgat
     6361 ctccgtcatc tcccgcggca ccctgatcca actggccacg cccgacccct acctcggccg
     6421 cgtgagctcc gtcgagaaca tcgtcggcgt cggcggaccg ggcatcggca acgccagggc
     6481 cggagcagtg gccagcctca cctcggcttc gaccgccgcg gtcaccggcg gcctcgcctg
     6541 cgccgtcgtc gtggtcgcgc tggccgccac aaaccccgcg ctgcggcgct ggcgacacca
     6601 gtacgcagaa ccagttgacc agccctcggg ctgaccggac tgagatcggc cgcgggcgga
     6661 gtaatgggca tcaggacccg acaccacagt gccggcggcg gacccctcac cccagacgga
     6721 cgggcagctg ggcgagccgg cgcgaccccg gtatctgcac ccactcctgc tgcgcgttga
     6781 ccagggccag gtccgggaac cgctcgaaca gggcgcacag cgccacctca ccctcctgcc
     6841 gggcgagcgc cgccccgagg cagtagtgga tgccgtgccc gaacccgacg tgtccctcgc
     6901 cgcgcccggt cggccgccgg gtcacgtcga gccgctcggg gtcggtgtac tcccggggat
     6961 cgaagttcgc ggacaccagc accgcctgca cggcgtcgcc ggcccggacc cggacgcccc
     7021 cgaggtcgag gtcctcggcg gcgtagcgca gccgcgtgat ctgcaccggc ccgcagaagc
     7081 gcatgagctc gtgcaccgca ccgggccaca gactggggtc gcggcgcagc aggtcgagct
     7141 ggtcggggtg ggtgagcagg gcgacggtgc cgttcccgat caggtgggcc gtggtctcgt
     7201 ggcccgccat gaccagggtc aacaccatcg tcaccagctc gtcgtcgctg agccggtcgc
     7261 cgtcctcatc ctgagcgtgg acgagcgcgg tcagcaggtc gtcggccggc tcggcgcggc
     7321 ggcgacggat gaggtcccgc acgtggtcga ccatgtcgcg caccgccccc gggatgactt
     7381 ccgggttcat cgacagcagg gcgatgcccc actcccgcca ggcgggccgg tcgacctccg
     7441 gtaccccgac cagctcgcag atcaccgtga tcggcagcgg gtaggcgaag tgctcgatca
     7501 ggtcgacggt ggacgggagg ccgtccagca gcgccgcggt gatcgcctcg acccggggtc
     7561 gcagctcgct gacccgccgc accgtgaacg cccgcgacac cagcttgcgc agccgggtgt
     7621 gatcggctcc gtcggcgtcg aggatcgatt cggtgaggta gtgggcgagc tcgcggggga
     7681 tgcccagctt ctcgagcgtc ttgtcgcgga cgttgtccac ctgcacgccg ggcaccgcgg
     7741 ccgggttgtt gacgaaccgc gggtcgccca gcacgaagcg cacgtcctgc tggcgcgtca
     7801 cgtaccaggc ctggccgccg tccatcgtgc gcccgcgcag cacggggccc tgctcgcgca
     7861 gccgtccgta gcccccatag ggatcggcga tcagggcggg gtccatcaga tccggttcga
     7921 cggacggggt cgtggtcatt cctggctccg tttccggtgc cgcatgctcc ggggtcaccg
     7981 gacgcggcgg cgccactcgt ggtcgatcag gtcgatgagt gatcggtaga gctcggcgac
     8041 gaccggcgcg agccgctcga gctcggcggt cggcgggtcg ggcacctgca tcgcggcggc
     8101 gagagcgtgc tcgaggaggt cggcacgggt ctccatgtcg agctcgggac gactccccgg
     8161 cgtcccaccc aggaagaaga acccggcccc gatcgccccg aacaggtaga gcagcgcgtc
     8221 gggctcgagg tcggtccgca ggcatcgtgc ctcgcgcagc agccgcaggt gttctcccag
     8281 cacttcctcg cggcgctcga ccagcccgcc gagggtgccc tcggcctcgt gcgcgagccg
     8341 gccgagcacc tcggcatcgc ccaggtacag ggcgcgggcc accgggtcgg cggcgagctg
     8401 caggaagacc gaccgcagca gccggctcgg cagcacgtgt gccggttcgg cctccatcag
     8461 gtcggccatc gccgtgacga tctggcgatg ggcgcgcagc agcaccgtga ggaacagcgc
     8521 gtccttggtg cggaagtgca ggtagacggt gcccttgccg attccggcgt gccgcgcgac
     8581 ctcgtcgatg gtgacccgct ggtaccccca ccgcaccagc agttcggcag cggtgtcgag
     8641 cacccgctcc atccggctgt tcgcgaactc cggagcggcc atgcgcaccc acccgtgtga
     8701 cgttgtgacc atctgacatt gtgaccagat ccggatatct ggtcacgagg tcacgatacg
     8761 cccggcgccg agggcgagca agaccgcttc gagacccctc acgcggctgg cgcgcacgcc
     8821 ggtccggact accgtgacga catggccgac tacgaacacc tgctggtcaa gcacgacggc
     8881 gacaccgtcc ggatcacgat gaaccggccg gagcggcgca acgcgctgtc ggagggccac
     8941 ctgcgcgagt tgctcgtcgc gttcgagacg gccgggcgca gcgacgccac ggggatcgtc
     9001 ctcgcagcag agggcaaggc gttctcggcg gggcacgact tcgccgacgt cgccgcccgc
     9061 gacctggccg gcgtccgcga cctgctgcag ctctgcaccc gcgtgatgcg caccgtccaa
     9121 gaggtgccgc aggtcgtcat cgcgcgggtg caggcgatcg cgacggctgc gggctgccag
     9181 ctcgtcgcgt cctgcgacct ggcggtggcc gctgagtcgg cggcgttcgc cctgcccggc
     9241 ggcaggggcg gctggttctg tcataccccg gccgttccgg tggcccgcag tgtggggcgc
     9301 aagaggctga tggagatggc cctcaccggc gacgcgatcg acgccgccac cgcggccgag
     9361 tggggactcg tgaactacgc ggtcccggac gccgagctgg acgcccgggt cgacgaactg
     9421 cttgcgcgct ccacccgtgg cagtcgcttc ggcaagggcg tcggcaagca gaccctctac
     9481 gcgcagctcg accggcccga ggccgacgcc tacgcgatcg ccgtcgaggt gatggcgtcg
     9541 ctgtcgcaga gctccggcgc acgggagggg atggcctcgt tcctggagaa gcgcccacct
     9601 acctggcccg actagctcgg tcgcggtcgc gacccacagc ggctcgggga cgtaacaagc
     9661 gtcatagacg caaccatgta tctatcctcg tacggtggat gcatgtcctc ctccacgagt
     9721 cgcgtgcctg attccgggcc ggcggcggac cgcgcctacg acctgaccaa gaggctcgtc
     9781 ctgtccggtg agttccccgg tgggcacctg ttcagcgagg gcgagatcgc cggtctgctc
     9841 gggatgagcc gcacccccgt ccgcgaggcg ttcctacgcc tgcaggccga ggagctgctg
     9901 cggctgatcc cgaagcgcgg agcggtcgtc gtgcccgtgt caccggagga ggcggaggac
     9961 gtcctcgacg cccgtgaggc catcgagatc gccgccgtgc gccgcttgct gacccgtcct
    10021 gatctcgtgc ccgctgccgt ggccgacctg cgggccgcac tgcgcatcca gcaggcccat
    10081 gccgacgcgg gtgacgtgca tgcgttcgcc gcggcggacg aggacttcca cggcaggatc
    10141 gtcgcagccg gacgcaacgg cctgaccatc cgcttctacg ccacgctcgc cgagcggcag
    10201 cagcggatga gcatgcgcgc gctcggaccg gtgcccgaac ggctgcccgg cgtgctgcgc
    10261 gagcacgccg agctgatcgc caccatcgag aacggcgacg agcagggttt cggcacggcg
    10321 ctgcgcgccc acctggacgg gatccaccgg tcatgacggc gcacaccccg gtgcaccagc
    10381 cggcggcagg ccgggcgacg gtgcaggcga cgggacgcag agcgtggcgg ccggtcgtga
    10441 tcgcgatgtt ctgctgcggt tggggcggga accagttcac cccgttgctg atcttgtaca
    10501 gggaggcggg ctactccgtc atcacggtcg acgcgctgct gggcacgtac gtcgtcggcc
    10561 tcgtcccggc cctgctgctg accgggggcc tctcgaaccg gtacgggcgc aaaccggtca
    10621 tgctcgccgg caccgccgtg gcgctactgg ccagcgtcct gctggcactc ggggagcacg
    10681 gcacgatctg gataatcgca ggccgcctgc tggccggcgc gggcgtgggg gtggcgatgg
    10741 ccgtcgggtc cacctgggtc aaggaactca ccgatgccga ccccgcttcc tcgccgggcc
    10801 tcggtacccg gcgtcccgcg ctgtggctga cgctcggcct cgcggccggc ccgggagtcg
    10861 ccggggtgct ggcccagtgg gcgccgtggc cgatggtcct gccgtacgtc gtccacatcg
    10921 tcatcaccgc ggtcgctctg ccgttcctgc tgcgcagccc cgagacgagc ggagggcctg
    10981 tcaccgcgcg cggtcgccgc tacaccggca tacggcaccc gcggttccgc cgagtgatca
    11041 cgccgatggc accgtggatc ttcggctcgg ccagcgtggc ctacgcgatc atgccgcagc
    11101 tcgtcgatag ccggctcggc tcctggggac tcgcctactc cacgctgctg gttgtctgca
    11161 cgctcggcgc aggcgtcgcg gtgcagccgc tggccaagcg cctcgaccgg acgacgagcg
    11221 cccgcggcgt cgtcgtcggg atgatcgtga tgtccgtagg gctcggcctc agcgccgtcg
    11281 ctgccgcgct ccgcatgccg ctgctcgtgc tcgccgtggc gatcgtgctc ggcgctgcat
    11341 acgggatcgc cgtcgtctcc gggctgctgg agctgcagcg cctggcccgc cccgaggagc
    11401 tcgccgagct caccggcgtg tactacgcgc tggcctacat cggcttcctg ttcccgtcgc
    11461 tgctcgcgtt gctcagcagc cttgccacct acacggcact gctctgcggc ctcgctgtca
    11521 ttgcgctggc gggaacgacg gtgatcgtgc ggcactcgcg ctcacacctg cccctgaaaa
    11581 gcggggtagg cgcctagcct cggctcagga ctgccttcag gaactccttc gtgcgcgggt
    11641 gttccggctc gctgaacagc cgctccggcg gcgcgtcctc gatcacctgg ccgccgtcga
    11701 acatcatcac ccggtgcgag aagtcgcggg cgaaccccat ctcgtgggtg acgcagagga
    11761 tggtgatgtc ggtggtctcg ccgatgtcgc gcagcagcgc gagcacgccg gcgacgagct
    11821 cggggtcgag cgcggaggtc acctcgtcga gcagcagcac gtcgggccgc atggccagcg
    11881 cccgcgcgat cgccacgcgc tgctgctggc cgccggacag ccgcgacggg tgctcgtcga
    11941 tccgctcccg gagcccgacg aggtcgagca gctcctcggc ccgggcgttg gcctcgtcct
    12001 tggacaggcc gagcacgcgg gtgggcgcct cgatcaggtt gccgcgcacc gtcatgttcg
    12061 ggaagaggtt gaactgctgg aacaccatcc cgatgcgctt gcggacctca cggctgtgct
    12121 tctcgtcggc cgggacgagc gtgccgccct tctccatgtg cgagaggtag cgcccggcga
    12181 ccctgatcgt gccggcgttc accgactcca ggcacatgag cagtcgcagg atcgtcgtct
    12241 tgcccgatcc cgacggcccg atcagcgtga cgtgctcgcc gggggcgacg gtgaagtcca
    12301 ggtcggagag cacgacgttg tcgccgaacc gcttccccac cccgtcgaac acgatcatgg
    12361 gctcagatgg ttccgacacg gcgttccaat ctccgcacca gcagcgaggc agggtagctc
    12421 aacaacagga agaggagccc cgcaatcgtg tacggctcga cgtagcggaa gtaggtgtcg
    12481 ccggcctgct gagcccggcc gacgacgtcg aggagcccga tcgcggtgag cagcggaacc
    12541 tccttgaaca tcgagatgac gtagttgccg agagcgggca cgacccgggg gatcgcctgc
    12601 ggcagcacca ccccggtcca gacccgcgag cgcggcaggc tcagcgccgt cgcggcctcc
    12661 cactgtccac gcggtaccgc ctcgatgccg gcgcggtaga cctccgacgc gtagcacgcg
    12721 tagtgcacgc cgagggcgat cacaccgacc gtcagcgcgc tcagcacgat gccgaactgc
    12781 ggcagcacga agtacaggaa gaagatctgc accagtaggg gcgtgctgcg cacgaactcg
    12841 acgaagccgc gcacggccac cgagagcagc ggcgggccct gccgcagcac cgccagcagc
    12901 agccccagga tcatcgcgac gaccgagccg agcacggttg ccagcaccgt gagccccagc
    12961 ccctccagca gcgaaggcag gatggcgaac gcgtagttcc agtcccagac ctgcgtcatc
    13021 ctcgccctcc cgtgctcgcg agccgcttgg ccctcgggga cgggccccgg ccgagccggg
    13081 ccgacgccac gcgttcgagg gcccgcatgc ccgccgtgat gagcagcgac agcaccaggt
    13141 agaccagcag caggagcagg aagatctgca gaacctgcgg gttgaaccgc gggacgagga
    13201 tctgcgtggc ctgccgggtg atctcgctga tgaacacgaa cgacaggatc gacgtgctct
    13261 tgagcagctg gatgaacagg ttgttgaagg gcgggatcat ctcgacgacg gcctgcggca
    13321 ggatcacccg gaacatccgc tgcgccgggg agaggctgag cgccaacgca ccctcgtatt
    13381 gcgcccgcgg caccgactgg atcgccccac gcacgatctc cgccccgtac gcgccgtggt
    13441 tgaggccgag cacgaggaca ccgccccaga tgggcacgat ctcgaacccg acgagcacgg
    13501 gcagcgcgaa gaagagccag aacagctgca cgacctccga cgtaccgcgc agcccctcca
    13561 cgtagatgcg gctgatcacc cgcaccgggc gcgacggcga cagcaacgcg agcccggccg
    13621 cgaacgacag caggatcgtc agaacgatcc ccccgacggt ggcgatcagc gtctccggca
    13681 ggccttgggc gaggacggac aggaacgtcc cgaggttgtc caccgccggc tcaggccgcg
    13741 cagagctgct cggtggtcag tccggcagtc ggcaggttgg actgggagaa accgaacgga
    13801 ctcgcgatct gcacccagcg cccgttgccg tgcagggtct gcagctcggt gttgaacgca
    13861 ttccgcacgt cggtgtcggc ctgccggaac acgaacccgc ccgccgagac gacctcccgg
    13921 ccgttctgcg tgggcgtgaa gcccggggtg acctccacac ccgcctgcgg gttcttgccg
    13981 gcgagccact tcagcgagat gtcggtgagc gccgcggcgt agacccggcc cgcggtgacg
    14041 gcctgtagca aagcgttctg gtcgtcgaac gggctgatgt tgccgggtgg gacgcccgcc
    14101 gcctcggcgt acccctgctc gacggccgcg cccagcaccg cgagcggcag tttcttctcc
    14161 gcgatgtgct ggaaggtggt gaccccctgt gggttgccct tcggcacgag aaacgcggtg
    14221 agcgcggtgt agtccgggac cgagaaggac gcctgctggc accgctgggg cgtgatgttc
    14281 atgcccgcgc agaccatgtc gaagcggttg gcattgagcg ccgggatcag cgagccgaag
    14341 tcgacctgtt cggcctgcac gtcgcggatg ccgatgccct gcaggaccgc gcgggccacc
    14401 tccggggcct cgcccgtgac cctgccgtcg gtgccggtga agccgtaggg ctcctcaccg
    14461 gcgatcccga tgcgcatcga gccgctgttt cgcgcgttct ccagcacgct gcccccgccg
    14521 ctgctggtac tcgtacaggc ggagagcgcc gttatcccac cgagtgcgac cgcaccggcg
    14581 gcggtgcggc ggaggaaatc gcgcctgctc caagatcgct gcgtcatggt ggttcccttc
    14641 cggatcgaac gggcggcgtc aatgccagcc gttacggtcg accaaccgaa cgtaggtgaa
    14701 cacccagggc cacaccggac caggggatgt tgacatccga ctcgttaccc gaccgcaatc
    14761 cgggctgtcc gttccggaac gccggtcgcc cgagccgatc acgttccgat gcgcgaggat
    14821 cagccccatg ctgcgcatcg ccgggctgcc ggtctgggtc ggcccttccg aggtcatctc
    14881 cggcgcgcgg gcggtgatgg ggtggaccga cgaggcagtc gaggtcgtcg cgggactgcc
    14941 cgaacgggtc tctcggctgc tcgacgacgt cgacggcctc gtcgcccgga tcaccaccgt
    15001 cgccgaccgg gtggaggcca tcgccgaccg cgcggacggg atcgtgggat ccgtcgacga
    15061 ggtgctcgtc ggcgtgcgca ccgcggtcgc gagggtggac agcgtcctcg ccgacgccga
    15121 cggcatcgtc cgccgggtcg acccgctgct ggtcgaggtg cgcaccacgg cggacagtgc
    15181 cgccaccctc gtcgcacggg ccgggggcgt ggccgagggc gcggcagggc tggtcgcacg
    15241 cgcagacggc gtcgccgagg gcgcggccgt gctcatcgca cgcgccggtg aggtggccga
    15301 cggcgccgcg gagctcgtgg ccggagcggg cagggtcgcc gacagcgcag gtggcgtgat
    15361 cgagaaggcc accgcggtcg ccgaccgcgc cggcaccgtg gtggaccagg cgtccggcgc
    15421 agcggacggc gccacagagc tgctcgcggt ctaccagccg atcgcgcggc aggccgcacc
    15481 gctcgcccgg cgcttcgtgg aggagttctc cgaggaggag atccgggcag cgatccagct
    15541 ggtcgaccac ctcccccggt tcacggagca catggagtcc gacatcatgc cgatcctcgc
    15601 cacgctcgac cgcgtcggcc cggacgtaca cgatctgctc gaccagctca aggagatgcg
    15661 gcaggcgatc cagggcatcc cgggcctcgc gttcttccgg cggcgcggcg aggagcgcga
    15721 agaataaccc cccgtggggg gg
//
LOCUS       DASRSC010000264         3894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_434434,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000264 DASRSC010000000
VERSION     DASRSC010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434434"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..1301
                     /gene="murA"
                     /locus_tag="VFE39_13030"
     CDS             36..1301
                     /gene="murA"
                     /locus_tag="VFE39_13030"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET6259568.1"
                     /translation="MAEHFAVNGGARLVGAVDVVGAKNSVLKLMAAALLAEGTTTLRN
                     CPEILDVPLMADVLRSLGCTVDVDRDTVMIDVPAEPGAQADYRSVSKLRASVCVLGPL
                     VARCRRAIVPLPGGDAIGSRPLDMHQAGLRKLGAITEIEHGRVVAHAEELRGAQIWLD
                     FPSVGATENILMAAVLADGTTVIDNAAREPEIVDLCTMLQEMGAKIDGVGTSTLTVHG
                     VTGLQPTEHRVIGDRIVGATWAFAAAMTRGEVTVRGIDPHHIDLVLDKLRSAGARTEI
                     GTTEFTITMDGRPRAVDFVTLPFPGFATDLQPMAIALSAVADGTSMITENVFEARFRF
                     VDEMVRLGADARTDGHHAVVRGVDRLSSAPVWASDIRAGAGLVLAGLCADGQTDIWDI
                     EHIDRGYPRFVENLRGLGADIQRKSDPDR"
     gene            1382..2065
                     /locus_tag="VFE39_13035"
     CDS             1382..2065
                     /locus_tag="VFE39_13035"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HET6259569.1"
                     /translation="MITEWLGSLFAMLDALPAWQLYTVVGVLLVLETTVLVGLVTPGE
                     VVLLAAATTVGSAGEYAALAGVAACASMIGQTGGYLLGRRFGWRIRASRAGQWIGEPI
                     WVRAEGVLRGGTGRAITGSRFLAVAHSLVPVLAGTLRMPVRRFARYTALGAVIWALVY
                     VGLGSAASVALRQAAHLVGPTVTGVAVALVVVVLVVRAVRKRRAEQRARLTEHGVVGP
                     SEHPVTHVR"
     gene            2207..3025
                     /locus_tag="VFE39_13040"
     CDS             2207..3025
                     /locus_tag="VFE39_13040"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HET6259570.1"
                     /translation="MTTAAESASNSAKQAARSRPVKIGARVGIAAYGVTHLLIALLAL
                     QVAFGNRGERADQTGAFQSLAQQPFGQVLLWVLVAGFVAVALWSLSQAIWGYAYESDR
                     KKKIEKRAGSAGKALVYVVLAVLAARTAAGGGGGGGGQGATAGVLGLPGGQFIVGAVG
                     IGILVTGVVMVVRGVQKKFLEDMTLPPDRAARAAAVRAGKFGYIAKGITIALIGVLVV
                     IAAVRFRPQEASGLDVALKTLASQPSGPYLLIVVAIGLGAYGVFCFFDARYHRV"
     gene            complement(3036..3362)
                     /locus_tag="VFE39_13045"
     CDS             complement(3036..3362)
                     /locus_tag="VFE39_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259571.1"
                     /translation="MFPGAPLITLVGALLMMGILFLALRWTFGTGRNQPAPLVHDPDD
                     TTGDGLLEEVSRVPTEAAAQILRARLGRAGIRATIGRVDRAYRLLVFHDDLVPARIVV
                     SRGAIE"
     gene            3523..>3894
                     /locus_tag="VFE39_13050"
     CDS             3523..>3894
                     /locus_tag="VFE39_13050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HET6259572.1"
                     /translation="MPYPTDRERDRPWVIRTYAGHSSAERSNELYRRNLAKGQTGLSV
                     AFDLPTQTGYDPDHELATGEVGKVGVPVSHIGDMRRLFDGIPMAEANTSMTINAPAMW
                     LLALYVAVAEEQAAREAPHAVR"
BASE COUNT          543 a         1270 c         1478 g          603 t
ORIGIN      
        1 gtagccctcc gggggagggg atagcgtcgg tgcccgtggc agagcatttc gcggtcaacg
       61 gtggggcccg gctcgtcggc gcggtcgacg tggtcggagc gaagaacagc gtgttgaagc
      121 tgatggcggc agcgctgctg gccgagggca cgacgacgct caggaactgt ccggagatcc
      181 tggacgtccc cctgatggcc gacgtgctgc gcagcctcgg ctgcaccgtc gacgtcgacc
      241 gcgacaccgt catgatcgat gttccggccg agccgggcgc gcaggccgac tacagatcgg
      301 tgtcgaagtt gcgggcgtcc gtgtgcgtgc tcggcccgct cgtggcccgt tgccgtcgcg
      361 ccatcgtgcc gctgcccggt ggcgacgcga tcggttcccg tccgctcgac atgcaccagg
      421 ccgggctgcg aaagctcggc gccatcaccg agatcgagca cgggcgcgtg gtcgcccacg
      481 ccgaggagct gcgcggggcg cagatctggc tcgacttccc cagcgtcggc gccaccgaga
      541 acatcctgat ggccgccgtg ctcgccgacg gcaccaccgt gatcgacaac gcggcacgcg
      601 agccggagat cgtcgacctc tgcacgatgc tgcaggagat gggcgccaag atcgacggcg
      661 tcggcacctc gacgctgacc gtgcacggcg tcaccggatt gcagcccacc gagcaccgcg
      721 tgatcggcga ccgcatcgtc ggcgccacct gggcgttcgc ggcagcgatg acccgcggcg
      781 aggtgaccgt gcgcggcatc gacccgcacc acatcgatct ggtgctcgac aagctgcgca
      841 gcgcgggcgc gcgcaccgag atcggtacga ccgagttcac catcacgatg gacgggcggc
      901 cgcgggccgt cgacttcgtg acgctgccgt ttcccggttt cgccaccgac ctgcagccca
      961 tggcgatcgc gctgtccgct gtcgccgacg gcacgtcgat gatcaccgag aacgtgttcg
     1021 aggcgcggtt ccggttcgtc gacgagatgg tccggctcgg cgccgacgcg cgtacggacg
     1081 gccatcacgc cgtcgtgcgg ggcgtcgacc ggctgtcgag cgcgccggtg tgggccagcg
     1141 acatccgggc cggagcgggg ctcgtgctgg ccggcttgtg cgcggacggg cagaccgaca
     1201 tctgggacat cgagcacatc gaccgcggct acccgcggtt cgtcgagaac ctgcggggcc
     1261 tcggcgccga catccagcgg aagagcgatc cggaccgctg agctgatgcg ggggccaacc
     1321 ccacccgtac tcaggggtct ccccgatggg ccgggggcgc tcgccggacc agtctggacc
     1381 ggtgataacc gaatggctcg gctccttgtt cgcgatgctg gacgccctcc ctgcctggca
     1441 gctctacacc gtcgtcggcg tgcttctcgt gctggagacg acggtgctgg tcgggctcgt
     1501 gacgccaggt gaggtcgtcc tgctcgcggc ggccaccacg gtaggcagcg ccggtgagta
     1561 cgcggcgctg gcgggggtcg cagcctgcgc gagcatgatc gggcagaccg ggggatacct
     1621 gctcggccgg cggttcggtt ggcggatccg ggcgagccgc gccgggcagt ggatcggcga
     1681 gccgatctgg gtacgtgccg agggtgtcct gcggggcggc accggccggg cgatcactgg
     1741 ttcacggttc ctcgcggtgg cgcactcgct ggtgcccgtg ctggccggga cgctgcgcat
     1801 gcccgtgcgc cggttcgccc ggtacacggc gctgggagcc gtgatttggg ccctggtcta
     1861 cgtggggctg gggagcgcgg cgtccgtggc attgcggcag gccgcgcacc tggtcgggcc
     1921 gacggtgacg ggagtggcgg tggcgctggt ggtcgtcgtc ctggtcgtcc gggccgtgcg
     1981 caagcggcgt gcggagcagc gtgcgcggct gaccgagcac ggggtcgtgg gaccgagcga
     2041 gcatccggtg acgcacgtcc ggtgagtgcg accgacagcc ggtttgctga tcgcccggtc
     2101 gggggactgc tgggaaacgg ccgtggggcc ggacagagca gcagtggctg cggcgcgatc
     2161 acttcagtgc gacggcgcgc cctgtacacg gagaggacct ccaaccatga ccacagccgc
     2221 ggagtcggcc agcaattccg ccaagcaggc cgccaggagc cgacctgtca agatcggtgc
     2281 gcgggtgggg atcgcggcct acggcgtcac ccatctgctg atcgcgctgc tggccctgca
     2341 ggttgcgttc ggcaaccgcg gtgagcgggc cgaccagacc ggcgcgttcc agagcctcgc
     2401 gcagcagccg ttcggacagg tgctgctgtg ggtgctcgtc gccgggttcg tcgccgtggc
     2461 gttgtggagt ctctcccagg cgatatgggg ctacgcctac gagtccgacc gcaagaagaa
     2521 gatcgagaag cgggccggga gtgcgggtaa ggcgctggtc tacgtggtgc tggccgtgct
     2581 ggccgcgagg actgcagcgg gcggtggcgg tggaggtggt ggacagggcg ccacggcggg
     2641 ggtgttgggt ctgcccggcg gccagttcat cgtcggcgcg gtggggatcg ggatcctcgt
     2701 gacgggcgtc gtcatggtgg tgcggggcgt ccagaagaag ttcctcgagg acatgaccct
     2761 gccgcccgac cgcgcggcgc gcgcggccgc ggtgcgcgcc gggaagttcg gctacatcgc
     2821 gaagggcatc acgatcgctc tgatcggggt gctcgtcgtc atcgcggcgg tccggttccg
     2881 gccccaggag gcgtccggcc tggacgttgc gctcaaaacg ctcgcctcgc agccgtccgg
     2941 gccgtacctg ctgatcgtcg tggccatcgg gctgggcgca tacggtgtct tctgcttctt
     3001 cgacgcgcgg taccaccgcg tgtagctgac gctggtcact cgatggctcc gcggctcacg
     3061 acgatccgtg ccggcaccag gtcgtcgtgg aacaccagca ggcggtaagc gcggtcgacc
     3121 cggccgatcg tggcgcggat gccggccctg ccgagtcggg cccggagtat ctgcgcggcc
     3181 gcctccgtcg gcacccggga gacctcctcg agcagcccgt cgcccgtggt gtcgtcgggg
     3241 tcgtggacga gaggcgcggg ctggttgcgt cccgtgccga acgtccagcg cagggccagg
     3301 aagaggatgc ccatcatcag cagggcgccg acgagggtga tcagcggcgc gccggggaac
     3361 atgcggaaca ttgtgcggca ggaacgccgt gagcgcgcgg tcgcttttat cgatccggct
     3421 tcgccgcggg tgtgacggag gtcgttcggt tccgttcgtg tagctacgcg ccagtagcat
     3481 gagccaatcc tgttggccgg atccacgatg cggaggtgtc cggtgccgta cccgaccgac
     3541 cgtgagcgcg accgcccctg ggtgatccgg acctatgcgg gccactcctc agcggagcgg
     3601 tcgaacgagc tctaccggcg caatctggcc aagggccaga ccgggctgtc ggtggcgttc
     3661 gacctgccga cacagaccgg ctacgacccc gaccacgagc tggctacggg ggaggtcggc
     3721 aaggtcggcg tgccggtgag ccacatcggc gatatgcgca ggctgttcga cggcattccg
     3781 atggccgagg ccaacacctc gatgacgatc aacgcgcctg ccatgtggct gctggccctc
     3841 tacgtcgcgg ttgccgagga acaggcagca cgggaggcgc cacatgcagt gcgg
//
LOCUS       DASRSC010000265         3825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_267713,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000265 DASRSC010000000
VERSION     DASRSC010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267713"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VFE39_13055"
     CDS             <1..192
                     /locus_tag="VFE39_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259573.1"
                     /translation="AYEHAGVARARIADAVAVLSDLGEQHSEPLVPVELRRAADELER
                     GLGLISTGAAVVADIDARL"
     gene            199..3006
                     /locus_tag="VFE39_13060"
     CDS             199..3006
                     /locus_tag="VFE39_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HET6259574.1"
                     /translation="MAVGQNRRDRIAGAFEALCDAVARALGAAEVAREAAVQAHAEAM
                     VEMWLRTDGLDAVQVDPRLRWLLDNPRLTGPVERVRADRAAAVSPWLETGPQRLADIL
                     AGAAPGAAGREPSEWLGRFGEVGGDGPVPSLWLIGTGRIGTAAPGFPVAVPLLDESHL
                     QITSTHESRTRAEAMLESVLMRVISYFRPGLVQLHVWDVGQFTGALPGLYPLTRTGLL
                     TVHDPSRLPQLLEDLSDRIRRVHTRVLVDGHPSLRALAESHGGRSEPWIVAVLVGNRS
                     ALREEDHRQLQRVARGGLACGVQLVLLDVPMTVNAPVETVRLGDDGLASTSMTGPHVT
                     VEPEPQLPSGAVTSACHAISDEHETWRSRIGTFADLLPLPAHWGQSRSLAGLHAPVGF
                     SDGRPVEIALADASPHALIGGPSGSGKTNLLLTMISSMATRYNPDELEFYLLDFKEGV
                     SFAQFGPGRRDRTWLPHARLVGVNINTDREFGLALLQFLSDEMRRRAEAAKAHEVTKL
                     EELRAADPEGRWPRMVAVIDEFQYLFAERDAVTRAATLLLEDVSRRGRSQGIHLVLAS
                     QDVSGIEAFWGRPAIFEQFVLRIALPRARRVLADLNDATLDLPRWHAVLNHESGMKHG
                     NEVVRIPDASGTGSVAADEPRGRPSVHTVQHDLHDMYHTGQAEPRLFDGSRAPRFAEL
                     AADLSSGTDPAESAGGALTDAAPRALVGQSIDVAGSAAAVRLPSAPGRNLGVLASVGR
                     DAVQVLGAAAASLASQFRPGDVDVVIAPLVAEAAVPAARLRELVSAAGHTAEIVPLDG
                     VRARVDALAAEVNERLGGAVSRRPVVVVLYAADAADTVLEREGTESMRRLLRFGPETG
                     VHVLGWWRSVQRLRALLMMSASVDDLGAWVALDVQGSELQALVPGMMVNWSPRPGRGL
                     FFDRAQHAAPEVVIVPSLEEL"
BASE COUNT          481 a         1348 c         1432 g          564 t
ORIGIN      
        1 gcctatgagc acgccggggt cgcgcgcgcc aggatcgctg acgcggtcgc cgtcctctcc
       61 gacctgggcg agcagcactc cgagccgctg gtgccggtcg agctgcgtcg tgccgccgac
      121 gagctggagc gcgggctggg gctgatctcc accggcgcgg ccgtcgtcgc cgacatcgac
      181 gcacggctgt aggcggcagt ggccgtcgga cagaaccggc gcgatcggat cgccggggcg
      241 ttcgaggccc tgtgtgacgc cgtcgcacgg gcgctcggtg ccgccgaggt cgccagggaa
      301 gcggccgtgc aggcccacgc cgaggccatg gtcgagatgt ggttgcgcac cgacgggctc
      361 gacgccgtgc aggtcgaccc ccggctgcgc tggctgctcg acaaccctcg cctgaccggt
      421 ccggtggagc gggtgcgcgc ggatcgggcc gccgcggtct cgccgtggct cgagaccggg
      481 ccacagcggc tggccgacat cctcgccggc gcggcgcccg gcgcggcggg ccgcgagccg
      541 agcgagtggc tcggccggtt cggcgaggtc ggcggcgacg gcccggtgcc gtcgctgtgg
      601 ttgatcggca cgggccggat cgggaccgcg gctcccggct tcccggtggc cgtccccctg
      661 ctcgacgagt cgcacctgca gatcacctcc acccacgaga gccggacacg cgccgaggcg
      721 atgctcgaga gcgtgctgat gcgggtgatc agctacttcc ggccggggct ggtgcagctg
      781 cacgtctggg acgtcgggca gttcacaggt gcactgcccg ggctctaccc gctcacgcgt
      841 accgggctgc tcaccgtcca cgatcccagc aggctcccgc agttgctcga ggacctgtcc
      901 gaccggatca ggcgcgtgca cacccgggtg ctcgtcgacg gccatccgtc cttgcgtgcg
      961 ctcgccgagt cccacggcgg ccgcagcgaa ccgtggatcg tcgcggtgct cgtcgggaac
     1021 cggtcggcgc tgcgcgagga ggaccaccgc cagctgcagc gcgtggcgag gggcggtctc
     1081 gcctgcggcg tccagctggt gctgctcgat gtgcccatga ccgtcaacgc tccggtggag
     1141 accgtgcgcc ttggcgacga cggcctcgcc tcgacgtcga tgaccggtcc gcacgtcacg
     1201 gtggaaccgg agcctcagct gccttcaggc gcggtcacca gcgcgtgcca cgcgatctct
     1261 gacgagcacg agacgtggcg cagccggatc ggtaccttcg ccgacctgct gcctctgccc
     1321 gcccactggg gccagtcgcg ctctctcgcc ggtctgcacg ctccggttgg tttctcggac
     1381 gggcggccgg tggagatcgc gctggccgac gcgtccccgc acgccctgat cggggggccg
     1441 agcggctcgg gcaagacgaa cctcctgctc accatgatca gttcgatggc cacccgctac
     1501 aacccggacg aactggagtt ctacctcctc gacttcaagg agggcgtctc cttcgcccag
     1561 ttcgggcccg gtcgccggga ccgcacctgg ttgccgcatg cccgcctcgt cggcgtcaac
     1621 atcaacaccg accgtgagtt cgggctggcg ctgctgcagt tcctctccga cgagatgcgc
     1681 cgccgcgccg aggccgcgaa ggcgcacgag gtgaccaagc tggaggagct ccgggctgcc
     1741 gacccggagg gtcgctggcc gcggatggtg gctgtgatcg acgagttcca gtacctgttc
     1801 gccgagcggg acgccgtgac gagggcggcc acgctgctgt tggaggacgt ctcccgccgc
     1861 ggccgctccc agggcatcca cctcgtgctg gccagtcagg acgtctccgg gatcgaggcg
     1921 ttctggggcc gcccggcgat cttcgagcag ttcgtgctcc ggatcgcgct gccccgtgcg
     1981 cggcgggtgc tcgccgacct gaacgacgcg acgctcgacc tcccccgctg gcacgccgtg
     2041 ctcaatcacg agtccgggat gaagcacggc aacgaggtcg tgcgcatccc ggacgcctcc
     2101 ggcacgggct cggtcgccgc ggacgaaccg cgtgggcgcc cgagcgtcca cacggtgcag
     2161 cacgacctgc acgacatgta ccacaccgga caggcggagc ctcggctctt cgacggcagc
     2221 cgcgcgcccc ggttcgccga actcgccgca gatctgtcat cgggtacgga ccccgcggag
     2281 agcgccggcg gagcgttgac cgacgccgct ccgcgagccc tcgtcgggca gtccatcgac
     2341 gttgccggta gcgcggccgc cgtccggctg ccctcggcgc cggggcgcaa cctcggcgtg
     2401 ctcgcctccg tcggccggga cgcggtacag gtgctcggcg ccgcggcggc ctcgctcgcg
     2461 agccagttcc gccccggcga cgtcgacgtg gtgatcgccc ccctggtcgc cgaggcggcc
     2521 gtccccgctg cccggctccg ggagctcgtc agcgcggccg ggcacaccgc ggagatcgtg
     2581 ccgctcgacg gtgtccgcgc gcgggtcgat gctctcgccg ccgaggtgaa tgaacggctg
     2641 ggaggcgccg tgtcgcgccg gcctgtcgtg gtggtgctct acgccgccga cgcggcggac
     2701 accgtgctgg agcgcgaggg caccgagtcc atgcgccggc tgctgcgctt cgggccggag
     2761 accggcgtcc acgtgctggg gtggtggcgc agcgtgcagc ggctgcgggc gctgctgatg
     2821 atgagcgcgt cggtcgacga cctcggggct tgggtcgccc tggacgtcca gggttccgag
     2881 ctgcaggcgc tcgtgcccgg gatgatggtc aactggtcgc cgcgaccggg ccgcgggctg
     2941 ttcttcgacc gcgcccagca cgcggcgccg gaggtcgtca tcgtgccctc gctggaggag
     3001 ctgtgagcga gcttgcgagc gaatcatcga cacagcgccc gcgcgcagcg acgtttcccg
     3061 atggggccgg ctgagcgcag cgcgggcgtg cgaatgagtt gccctgtgtc aagctgcggc
     3121 cggttgatct tggttgagga tgcgttgaag ggcgccgtgg cggtccgcgc agttggttgg
     3181 cgatcgcgac tcggtgcgcc accaggtcgc ggcgcgctcg gacggtctgg cgcagcgcgg
     3241 tggtgggttc ggtgtcggcc agcagcgggc gcagccggcg ccgttcgatc cggacgcggc
     3301 cacatcggcg gccagcagat cacggaccag ggtgcgcagc ccggccgcgt cgtgtgcgac
     3361 cgggaaccgg cgggtctgcc cgccgtcggc gtcgaagatg gctacggcgt gatcgtgttg
     3421 ggcccagtcg acccccgcgg tgggtcgcgc gggttcgatg ggatcggtga cactcatcgt
     3481 tgtctcctcg ctgttggcgc agtgggaaga cacccggtgg tcccgggaca cggctgccgg
     3541 tcgctcactg atcggcgctc gatggcgcac agccctgtag ccagtcggcg cgtcctgggc
     3601 caccggacct cgcagaactc atcgttgacc tcaagggtcg agcgagctgg gcgatggccc
     3661 ggtggggacc ccaggtgcgc ccgaatcctg gatcaacaac caggctcgga cacaaggagg
     3721 gtgcaccagt gagcgaggct gacggcgacg gcgtggcggg ggagcaccgg gcgccctacc
     3781 gggcggtcgg tgggcacgag gccgccggtg agcacaaggc cggtc
//
LOCUS       DASRSC010000266         2542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1580396,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000266 DASRSC010000000
VERSION     DASRSC010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580396"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..672
                     /locus_tag="VFE39_13065"
     CDS             259..672
                     /locus_tag="VFE39_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6259575.1"
                     /translation="MSIVAGAGPAGASAGEAAHAQVDDQTAGHTTAHRSAFPAPVRTP
                     RTLAAAGDLLRALAAPVRIAIVLQLLESDRCVHDLVDALGVAQPLISQHLRVLKSAGV
                     VHGERQGREVVYSLVDDHLAHIVVDAVAHAEEEVR"
     gene            669..1091
                     /locus_tag="VFE39_13070"
     CDS             669..1091
                     /locus_tag="VFE39_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HET6259576.1"
                     /translation="MSAAGQAPTTRALRATRQRAAVSTLLDRLDDFRSAQEIHEELRR
                     RGEGIGLTTVYRTLQTLADSGEVDVLRTGTGEAVYRRCDTVDHHHHLVCRRCGYTVEI
                     EGPGVESWAQQVADTHGFVELSHTAEIFGLCKDCGAGR"
     gene            1163..1795
                     /locus_tag="VFE39_13075"
     CDS             1163..1795
                     /locus_tag="VFE39_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259577.1"
                     /translation="MADDVAGTNGAATATAPSTVRRYDSPESLELAALAAVFEDLQYV
                     LRCCEHLVTALTAPDSGPVQVDVDTALVEALWTGALIGYVRCFSGRTAVLTNDDLDQL
                     GLEGDVGDFHAMIKKLRDHYASRHVNPRESFTIGAAQANDGRPTGVAVVSTPRPIVDD
                     TTVRLLGRVAYALSGLVDARMQEAQARVLAAAATLTPAELARLPLVHLSS"
     gene            complement(1821..2225)
                     /locus_tag="VFE39_13080"
     CDS             complement(1821..2225)
                     /locus_tag="VFE39_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259578.1"
                     /translation="MSSSAIPDMLGVARAALERFHDVTVDDDGALTFAHGGVVCALQG
                     LELEEGLPVLNLTCVVAWDLPPESDIPTRVAFGAGEGLFGTPRVIRGQAGWDVTLRYA
                     FPAVGLEEKAMGTLLMLVVSSASSMRTELTES"
     gene            complement(2222..>2542)
                     /locus_tag="VFE39_13085"
     CDS             complement(2222..>2542)
                     /locus_tag="VFE39_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HET6259579.1"
                     /translation="AEIADSVRQIARLVAEGRIKPDKIDDRTIARYLDEPDMPDVDLF
                     VRSSGEQRTSNFLLWQSAYAEMVFLDTLFPDFDRRHLWEAIEIYARRDRRFGGALDKP
                     GAAS"
BASE COUNT          371 a          917 c          917 g          337 t
ORIGIN      
        1 gcacggcgag cacccagtgc tccggctcgt cgatcgagcg ccccagctca cccccgaggc
       61 agccgtccgc ggcggtgagc agctgaagcg cccggcgcgc ccgtgagagg agcgcggggg
      121 cgtcggcggg agcggcaacg aaccggcaga cgagcagcac ggacggagca tgccaccgca
      181 cgcctacgat ggggcctgcg gccgggggag agctggacca ccggggggcg tggccgccgc
      241 tcccggggat ggggacccat gtcgatcgtc gctggtgccg ggcctgctgg ggcgtccgcc
      301 ggggaggcag cgcacgctca ggtcgacgac cagacggctg gacacaccac cgcgcaccgc
      361 tccgccttcc ccgcgcccgt tcgtaccccg cgcacgctcg cggcggcagg cgacctgctg
      421 cgggcgctgg ccgctccggt ccggatcgcc atcgtgctgc agttgctgga gtccgaccgg
      481 tgcgtgcacg atctcgtcga cgcgctcggc gtcgcccagc cgctgatcag ccagcacctg
      541 cgggtgctca agtcggcggg cgtggtgcac ggcgagcgtc agggccgcga ggtggtctac
      601 tcgctggtgg acgaccacct cgcgcacatc gtcgtcgacg ccgtggcgca tgcggaggag
      661 gaggtcagat gagtgccgcc gggcaggctc cgacgacgcg ggcgctgcgg gccacacggc
      721 agcgggcggc ggtgtccacg ttgctcgacc gcctcgacga cttccgctcg gcccaggaga
      781 tccacgagga actccgccgc cgcggcgagg gcatcgggct caccaccgtg taccgcacgc
      841 tgcagacgct cgccgacagc ggcgaggtcg acgtgctgcg caccggtacc ggtgaggccg
      901 tctaccgccg ctgcgacacc gtcgatcacc accaccacct ggtgtgccgg cgctgcggct
      961 acacggtcga gatcgagggg cccggcgtgg agagctgggc gcaacaggtc gccgacacgc
     1021 acggcttcgt cgagctcagc cacaccgccg agatcttcgg cctgtgcaag gactgcggcg
     1081 ccggccgctg aggccgtctc agaacgacac atccggctcg ccccgcggcc gctagcgtcc
     1141 ggcgcgacgc gaggaggcgc tcatggcgga cgacgtggca ggcaccaacg gggcggccac
     1201 agccaccgca ccgagcaccg tgcggaggta cgacagcccg gagtcgctgg agctggccgc
     1261 cctggcggcg gtgttcgagg acctgcagta cgtgctgcgc tgctgcgagc acctggtcac
     1321 ggcgctgacc gcgcccgact ccggcccggt ccaggtggac gtcgacaccg cgctggtcga
     1381 agcgctctgg acgggggcgc tcatcggcta cgtccgctgc ttctccggcc gcaccgcggt
     1441 gctgacgaac gacgacctcg accagctcgg gctggagggc gacgtcggcg acttccacgc
     1501 gatgatcaag aagctgcgtg accactacgc gtcccggcac gtgaacccgc gcgagtcgtt
     1561 cacgatcggt gccgcgcagg ccaacgacgg caggccgacc ggcgtggcgg tggtgtcgac
     1621 cccgcgcccc atcgttgacg acaccaccgt gcggctgctc ggtcgggtcg cctacgcgct
     1681 gagcgggctg gtcgacgccc ggatgcagga ggcccaggcc cgggtgctag cagcggccgc
     1741 gacactcacg cctgccgagc tggcccggct tccgctcgta cacctgtcct cgtagccccc
     1801 gtgccttcgt ttcctcgaca ctagctttcg gtcagctcgg tccgcatgct cgacgcgctg
     1861 ctcacgacca gcatcaacag cgtgcccatg gccttctcct cgagcccgac ggcggggaat
     1921 gcgtagcgca gtgtgacatc ccagccggcc tgcccgcgga tcacgcgagg cgtgccgaac
     1981 aggccctccc cggcgccgaa cgccacccgg gtggggatgt cggactccgg gggtaggtcc
     2041 caggccacga cgcaggtgag gttgaggacg ggcagtccct cctccagctc gaggccctgc
     2101 agggcgcaca cgacaccgcc gtgggcgaat gtgagcgcgc cgtcgtcgtc gacggtgacg
     2161 tcgtggaagc gctccagcgc cgcgcgggcg acgccgagca tgtccgggat cgcgctgctg
     2221 ctcatgacgc agcgcccggc ttgtcgagcg ccccgccgaa gcgccggtcg cggcgcgcgt
     2281 agatctcgat ggcctcccac aggtggcgcc ggtcgaagtc ggggaacagc gtgtcgagga
     2341 agaccatctc ggcgtaggcc gactgccaga gcaggaagtt cgacgtgcgc tgctcacccg
     2401 aggagcgcac gaacaggtcg acgtcgggca tatccggctc gtcgaggtac cgcgcgatcg
     2461 tccgatcgtc gatcttgtcg ggcttgatcc gcccctccgc gacgagccgg gcgatctgcc
     2521 gcacagagtc ggcgatctcg gc
//
LOCUS       DASRSC010000267         8069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4199913,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000267 DASRSC010000000
VERSION     DASRSC010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4199913"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VFE39_13090"
     CDS             complement(<1..547)
                     /locus_tag="VFE39_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259580.1"
                     /translation="MVAPRSLPDAVGTLFESTPRADSPAYRGRIERLAAQWAELGPPG
                     DPGVAAHLATLLRKLAATLRAEPFWPLAAREIGAELLATGLCGGPGRPIYGFDDVIAP
                     SLRLLRGEAAAVIGMRGPEGERRLVAVLDELVAGVVRSLHELNGAEQRADARMPPVTD
                     RHVRAVYEQSSFGMVILGLDGR"
     gene            complement(674..1030)
                     /locus_tag="VFE39_13095"
     CDS             complement(674..1030)
                     /locus_tag="VFE39_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterol carrier family protein"
                     /protein_id="HET6259581.1"
                     /translation="MPEEIDHGALDAVLDWLDGGPPPERSVLRSAVKESLAAVAAAAP
                     GRSVEVRVPPHGAVQCVSGPRHGRGTPPNVVETDPRTWLALVTGRLTWSEAVQAGTLR
                     ASGTRSGEIACLLPLS"
     gene            complement(1100..3379)
                     /gene="purL"
                     /locus_tag="VFE39_13100"
     CDS             complement(1100..3379)
                     /gene="purL"
                     /locus_tag="VFE39_13100"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HET6259582.1"
                     /translation="MTDTVQNATATPDHSQPYRELGLKDDEYARIREILGRRPTDAEL
                     AMYAVMWSEHCSYKSSKVHLAYFSETTTEEMRERMLAGIGENAGVVEIGDGWAVTFKV
                     ESHNHPSYVEPYQGAATGVGGIVRDIMAMGARPVAVADPLRFGPADAPDTRRVLPGVV
                     AGVGGYGNSLGLPNIGGEVVFDPGYLGNPLVNALCVGAMRTEDLHLAFASGTGNRIIL
                     FGARTGLDGIGGVSVLASETFSANEDGEEESASGRKKLPSVQVGDPFTEKVLIECCLE
                     LFHAGLVVGIQDLGGAGLSCATSELASAGDGGMRVDLDAVPLRASGMTPAEILSSESQ
                     ERMCAVVQPQDVDAFMAVCRKWDVLANVIGEVTDGDRLVISWQGETVVDVPPRTVAHD
                     GPVYRRPVQRSDRQHALVADTADRLLRPTAPAELRAEILRMAASPNLCSRAWVTDQYD
                     RYVRGNTASAQPADAGMVRIDEETGRGVAVATDCNSRFVALDPYAGAQLALAEAYRNV
                     ATSGAVPLAVTNCLNFGSPEDPHVMWQFEQAVRGLADGCAQLGIPVTGGNVSFYNQTG
                     DRAILPTPVVGVLGVIDDVARRVPSGFTRAGDVVLLFGDTRDELGGSEWANVVHGHLG
                     GRPPVVDLAAEQHLAGLLAAAAADGLLTGAHDLSDGGLAQALVEACLVGRQGAAVELP
                     PALDPFVALFSESAGRVLVTAAPDRVDEVRSMASAAGVAVTTLGTSGGAALTVFGLPE
                     LTLAELRSAWEGTLPGLFG"
     gene            complement(3483..4127)
                     /locus_tag="VFE39_13105"
     CDS             complement(3483..4127)
                     /locus_tag="VFE39_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6259583.1"
                     /translation="MGAPTSRAAKAAETEAALKAAAKRIFAERGYVNTKITDITAAAG
                     RAAGSFYNHFASKDELLAALLTDMFAESDLVMDDPDHRADFSDRSAVRWHIAQYWTFY
                     RRHLPEMVALRQAAMANPEFGRRLQEMVSADAAHLVGHLEPVRAAGIPLPGEPDVVVS
                     AFHSLLGQFAYTWLAAGGDGTGREISDEEAIETLTEFVHRGLAGTPPPASDRDR"
     gene            4214..5713
                     /locus_tag="VFE39_13110"
     CDS             4214..5713
                     /locus_tag="VFE39_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET6259584.1"
                     /translation="MEQTLLIVGAGPTGLTLGIELARRGVAVRVVDRSERPAIGSRGD
                     GLQPRTLEVFDDLGVLDAVRDAGAPHAPIRVHVGGRFVAERRMSEWREPTPDVPLPNP
                     WVLGQDRTEGILRDRLGELGVPVEFGTELVAFEQDDDGAIATLGRNGRSERIRAAYLV
                     GADGGRSTVRKALGIPFDGTTDESTRMLLGDVLVNGLDHEHGYWFAAADDPRSGIMLS
                     PLPGGDLFQAACAADADAEPSPATLQAAFDRFCRPLDVRLRELAWVTVWRPNSRLAQR
                     FRDGRVFLAGDAAHTHPPTGGQGLNTGVQDAYNLGWKLAEALDADAARAGDLLDSYEA
                     ERRPVAARVLGISDALLRKYTDGEADAHERGTETHQLELAYRDSPLVRDEREKPGRLR
                     AGDRAPDAPVVDADGRAMQLFDLFRGPHATALAFGGDGEALPGVPVHSVLRAGERATG
                     TAVVDAGGHAFSAYDVADGTTVLVRPDGYVGSITYSPRRVSLPSGASPR"
     gene            complement(5682..6125)
                     /locus_tag="VFE39_13115"
     CDS             complement(5682..6125)
                     /locus_tag="VFE39_13115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018680785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6259585.1"
                     /translation="MTELQPRIINVLLVEDDPGDVLMTREAFDEYLHNRLDVVTDGAA
                     ALSYLRSEEPYADMPRPDLVLLDLNLPRCDGREVLQEVKSDPDLQHIPVIVLTTSQAE
                     EDVLRSYQLHANAYVTKPVDFEGFIEAIKQIDHFFVSVVKLPTGG"
     gene            complement(6122..7702)
                     /locus_tag="VFE39_13120"
     CDS             complement(6122..7702)
                     /locus_tag="VFE39_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003099486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6259586.1"
                     /translation="MTALDRTPPERSGEARLQRLEEGRKHWPLARIFALGASLAALFA
                     LVVVVLGGVALWRLAEARIALVDSAGPALIAAQQLSTALVDQETGVRGYSLAQRAEFL
                     QPYQDGRATEAEMVAHLRELAAGGRLPGLDGRLDAVERAAQDWRTEYAGPVLAGTPGG
                     PDPERGRALFEQVRSAVQTMLADLQVEREAARVSIEGASAFLLVVGVAIAALLASFLV
                     AAGIGLRRGMLRPLSDLAVQVREVVTGDATQPVRAGGPREIVGLGADVEAMRQHIQRD
                     LDDAQAANRRLDEQARELERSNRDLEQFAYVASHDLQEPLRKVSSFCQLLQRRYAGKL
                     DDRADQYIAFAVDGASRMQRLINDLLTFSRVGRTTENFVALDLGETAAAAAAQLEPAR
                     EESDGEIDIGELPVVAGDPALLQALLVNLLGNGLKFHRAGVAPVVRVASRRDGDSFEI
                     TISDNGIGIESEYSGKVFVIFQRLHGRDVYPGTGIGLALAKKIVEFHGGRIDLADTPG
                     PGTTVRFTLPVPREEIDA"
     gene            7846..>8069
                     /locus_tag="VFE39_13125"
     CDS             7846..>8069
                     /locus_tag="VFE39_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6259587.1"
                     /translation="MERAAEDWRTEYAAPVLAGTPGGSDPERGRALFEQVRSAVQAML
                     ADLQAEREAARVGIEGASAFLLVVGVAIAA"
BASE COUNT         1153 a         2904 c         2869 g         1143 t
ORIGIN      
        1 cgcgaccgtc caggcctaag atcaccatgc cgaaggacga ctgctcgtac accgcgcgga
       61 cgtgccgatc cgtgaccggc ggcatccggg catcggcccg ctgttcggca ccgttcaact
      121 cgtgcagcga acggacgacg cctgccacca gttcgtcgag cacggcgacg agccgccgct
      181 cgccctccgg gccgcgcatc ccgatcaccg ctgccgcctc gccccgcagc aggcggaggc
      241 tgggcgcgat cacgtcgtcg aacccgtaga tcggccggcc agggccgccg cacagcccgg
      301 tggcgagcag ctcggcaccg atctccctcg cggcgagcgg ccagaacggt tcggctcgca
      361 gtgtcgctgc gagcttgcgg agcagagtgg cgaggtgagc ggccacgccg gggtcgcccg
      421 gcggacccag ctcggcccac tgggcggcga gccgttcgat gcggccgcgg tacgccgggg
      481 agtctgctcg aggagttgat tcgaagaggg tgccgacggc gtcaggaagg ctgcgcggtg
      541 ccaccacgcg gccacctccc gtcgcctatc cggtcctctt cggccctgat cgcgcaattt
      601 tccgtgtcgt gctgtgtttg cgccaccacc atgtgttggc attcacccgg ggggtgggtg
      661 gacggaagcc cggtcaggac agcggaagca gacacgcgat ctcgccgctg cgggtgccgg
      721 aagcgcgcaa tgttccagcc tgcaccgctt ctgaccaggt cagtcgtccg gtcacgagcg
      781 cgagccaggt cctgggatcg gtctcgacga cgttgggcgg ggtaccgcgg ccgtgccgcg
      841 gcccgctgac gcactgcacg gccccgtgtg gggggacccg tacctcgacg cttcgccccg
      901 gcgccgctgc cgcgacggca gccaggctct ccttgaccgc cgaacgcagc acggaccgtt
      961 ccggtggcgg cccaccgtcg agccagtcca gcacggcatc gagcgcgccg tgatcgatct
     1021 cctccggcat gaggcgaacg ctatcccgca cgagtggcac ctcacccctc ggagaggtgc
     1081 cggcttgccc gaccgcgggt cagccgaaca gacccggcag ggttccctcc cacgccgacc
     1141 gcagctccgc cagcgtcagc tccggaagac cgaagacggt cagcgcggcg ccccctgaag
     1201 tacccagcgt cgtgaccgcc acgcccgcgg ccgacgccat cgaccggacc tcgtccaccc
     1261 ggtccggggc ggccgtcacg agcacgcgcc ccgccgactc cgagaacaac gcgacgaacg
     1321 ggtcgagggc cggcggcagc tccaccgctg cgccctgcct gccgacgagg catgcctcca
     1381 cgagagcctg cgcgagacct ccgtcggaca ggtcgtgggc tccggtgagc agcccgtccg
     1441 ccgccgctgc ggcgagcagg cctgcgaggt gctgctcggc cgcgagatcg accaccggcg
     1501 gccgcccacc caggtggccg tgcacgacgt tcgcccactc gctgccaccg agctcgtcgc
     1561 gggtgtcgcc gaacagcaga accacgtcgc cggcccgcgt gaacccggac gggacgcggc
     1621 gggccacatc gtcgatcacg ccgagcacgc cgaccaccgg ggttggcagg atggcccggt
     1681 cgccggtctg gttgtagaag ctcacgttgc cgccggtcac cgggatgccc agctgcgcgc
     1741 agccgtcggc gaggccgcgc acggcctgct cgaactgcca catcacgtgc gggtcctcgg
     1801 gcgagccgaa gttcaggcag ttggtgacgg ccagcggcac cgctccgctc gtcgcgacgt
     1861 tgcggtaggc ctcggccagc gccagctggg cacccgcgta gggatcgagc gcgacgaacc
     1921 gggagttgca gtcggtggcg acggccaccc cgcgcccggt ctcctcgtcg attcgcacca
     1981 tcccggcgtc ggcgggctgt gccgatgcgg tgttgccgcg gacgtagcgg tcgtactggt
     2041 cggtgaccca cgcccggctg cacaggttgg ggctcgcagc catgcgcagg atctccgcgc
     2101 gcagctcggc cggggccgtg ggccgcagca gccggtcggc ggtgtcggcg acgagcgcgt
     2161 gctggcggtc ggagcgctgc acgggccgcc ggtagaccgg cccgtcgtgc gccacggtgc
     2221 gcggcgggac gtccaccacg gtctcgccct gccagctgat gacgagccgg tctccgtcgg
     2281 tgacctcgcc gatgacgttc gcgagcacgt cccactttcg gcacacggcc atgaaggcgt
     2341 ccacgtcctg cggttgcacg acggcgcaca tgcgctcctg cgactcgctg gacaggatct
     2401 cggccggtgt catcccgctg gcccgcaacg gcacggcgtc caggtcgacc cgcatgccgc
     2461 cgtcacccgc actcgccagc tcggacgtgg cacaggacag cccggcgccg ccgagatcct
     2521 ggatgcccac cacgagacct gcgtggaaca gctccaggca gcactcgatg agcaccttct
     2581 cggtgaacgg gtcacccacc tgcaccgacg gcagcttctt gcgcccgctc gccgactcct
     2641 cttccccatc ctcgttggcg ctgaaggtct cgctggcgag caccgagacg ccgccgatgc
     2701 cgtccagccc tgtgcgagcg ccgaacagaa tgatcctgtt gcccgtgccg gacgcgaagg
     2761 cgagatgcag gtcctcggtg cgcatggctc cgacgcacag cgcgttgacc agaggatttc
     2821 ccaggtagcc ggggtcgaag acgacctcgc cgccgatgtt gggcagaccg agtgagttgc
     2881 cgtagccgcc cactccggcg accacgccgg gcagcacccg gcgggtgtcc ggcgcgtcgg
     2941 ccgggccgaa acgcagcggg tcggccacgg ctaccgggcg agcgcccatc gccatgatgt
     3001 cgcgcacgat cccgccgacg cccgtggccg caccctgata gggctccacg tacgacgggt
     3061 ggttgtgcga ctcgaccttg aaggtgaccg cccagccgtc gccgatctcg acgacgccgg
     3121 cgttctcccc gatcccggcg agcatccgct cgcgcatctc ctccgtcgtg gtctcactga
     3181 agtacgccag gtgcaccttg gaggatttgt aggagcagtg ctcgctccac atcacggcgt
     3241 acatcgccag ctcggcgtcg gtggggcggc gtcccaggat ctcccggatg cgtgcgtact
     3301 cgtcgtcctt cagcccgagc tcgcggtagg gctgcgagtg gtccggggtc gcggtggcgt
     3361 tctggacggt gtcggtgatc cgcacggcgg cggtcggcgc ggtggcggcg gatgtcacgg
     3421 ttcccagcct acgtacagaa gtggggccgc cggcccgcgc ctcggtcggg cgacagcgcc
     3481 ggctaccggt cccgatcgct ggccggaggt ggcgtgccgg cgagcccgcg gtggacgaac
     3541 tccgtcagcg tctcgatcgc ctcctcgtcg gagatctcgc gccccgtccc gtcaccgcct
     3601 gccgccagcc acgtgtacgc gaactggccg agcaggctgt ggaacgccga caccaccaca
     3661 tcgggctccc ccgggagtgg gatcccggcg gcacgcacgg gctcgaggtg gccgaccaga
     3721 tgggcggcgt cggcgctcac catctcctgc aaccgccgcc cgaactccgg gttggccatc
     3781 gcggcctggc gcagagccac catctccggg aggtgcctcc gatagaaggt ccagtactgc
     3841 gcgatgtgcc agcgcaccgc ggaccggtcg gagaagtcgg cacggtgatc ggggtcgtcc
     3901 atgaccaggt cgctctcggc gaacatgtcg gtgagcagcg ccgcgagcag ttcgtccttg
     3961 ctcgcgaagt ggttgtagaa cgacccggcc gcgcgccctg ccgccgccgt gatgtccgtg
     4021 atcttggtgt tgacgtaccc gcgctcggcg aagatgcgct tggccgccgc cttcagcgcc
     4081 gcctcggtct ccgctgcctt ggccgcgcgg ctcgtgggag cgcccaatgc ccgacctcct
     4141 tgacggcgaa cgttagcagc gccgatgatg aacctagatt cagtgaactt aagttcatca
     4201 agtggaggta cccgtggaac agacgctcct catcgtcggc gccggcccga ccggcctcac
     4261 cctcggcatc gagctcgcac gccgtggcgt cgcggtacgg gtcgtcgacc gcagcgagcg
     4321 acccgcaatc ggttcgcggg gggacggtct gcagccacgc acgctggagg tcttcgacga
     4381 cctcggcgtg ctcgacgcgg tgcgggacgc cggcgccccg catgcgccga tccgggtcca
     4441 cgtgggcggc cggttcgtcg ccgagcggcg gatgagcgag tggcgggagc ccacgcccga
     4501 cgtaccgctg ccgaacccat gggtactcgg gcaggaccgc accgagggga tcctgcgcga
     4561 ccggctcggc gagctgggcg tcccggtcga gttcggcacc gagctggtgg cgttcgagca
     4621 ggacgacgac ggggcgatcg ccacgctcgg acggaacgga cggtcggagc gcatccgggc
     4681 ggcatatctg gtgggcgccg acggaggccg gagcaccgtg cgcaaggcgc tcggcatccc
     4741 gttcgacggc acgacggacg agtcgacccg gatgctgctc ggcgacgtgc tggtcaacgg
     4801 cctcgaccac gagcacggct actggttcgc cgccgcggac gacccgcgct ccggcatcat
     4861 gctcagcccg ctgcccggcg gcgacctctt ccaggccgcc tgcgcggccg acgccgacgc
     4921 cgaaccgtcc ccggccacgc tccaggcggc gttcgaccgg ttctgccggc cattggacgt
     4981 ccggctgcgg gagctggcct gggtcacggt ctggcggccg aactcccggc tcgcccaacg
     5041 gttccgcgac gggcgggtgt tcctcgccgg cgacgccgcc cacacccacc cgcccacggg
     5101 cgggcagggc ctcaacaccg gcgtgcagga cgcctacaac ctcggctgga aactggccga
     5161 ggcgctggac gccgacgccg cgcgggcggg cgacctgctg gacagctacg aggcggagcg
     5221 gcggccggtc gcggcgcggg tgctgggcat cagcgatgcg ctgctgcgca agtacaccga
     5281 cggcgaggcc gacgcccacg agcgcggcac ggagacccac cagctggaac tggcgtaccg
     5341 ggacagcccc ctggtgcgcg acgagcggga gaagccgggg cggctgcggg cgggcgaccg
     5401 cgctccggac gcgccggtcg ttgacgcgga tgggcgggcg atgcagctgt tcgacctgtt
     5461 ccggggcccg catgcgacgg cattggcgtt cggcggggat ggggaggcac tgccaggagt
     5521 gccggtgcac tccgtgctcc gcgccgggga gcgcgcgacc gggactgcgg tcgtggacgc
     5581 gggcggacat gcgttcagcg cgtatgacgt ggcggacggc acgacggtgc tcgtacgccc
     5641 ggacggttat gtcgggtcga tcacgtactc cccccggcgc gtcagcctcc cgtcgggagc
     5701 ttcaccacgc tgacgaagaa gtggtcgatc tgcttgatgg cctcgatgaa gccctcgaag
     5761 tccaccggct tggtgacgta ggcgttggcg tgcagctggt agctgcgcag cacgtcctcc
     5821 tcggcctgcg aggtggtgag gacgatcacc gggatgtgct gcaggtccgg atccgacttc
     5881 acctcctgca gcacctcgcg tccgtcgcag cggggcaggt tgaggtcgag caggaccagg
     5941 tccgggcgtg gcatatcggc gtagggctcc tcgctgcgca ggtaggacag tgccgccgcg
     6001 ccgtccgtca ccacgtcgag ccggttgtgg aggtactcgt cgaacgcctc gcgggtcatc
     6061 aggacgtcgc cggggtcatc ctcgaccagc aggacgttga tgatccgggg ttggagctcg
     6121 gtcacgcgtc gatctcctcg cgggggacgg gaagggtgaa gcgcacggta gtgccgggcc
     6181 cgggggtgtc ggcgagatcg atcctgccgc cgtggaactc gacgatcttc ttcgcgagcg
     6241 ccagcccgat accggttccg gggtagacgt cccggccgtg cagccgctgg aagatcacga
     6301 acaccttgcc ggagtactcg gactcgatgc cgatgccgtt gtcgctgatg gtgatctcga
     6361 aggagtcgcc gtcccgccgg gacgccaccc ggaccaccgg cgccacgccg gcgcggtgaa
     6421 acttcagtcc gttgcccagc aggttcacca gcagcgcctg caacagggcg gggtcgccgg
     6481 cgaccaccgg cagctcaccg atgtcgatct caccgtccga ctcctctcgg gcgggttcca
     6541 gctgggcggc tgcggcggcc gccgtctcac cgagatcgag cgcgacgaag ttctcggtcg
     6601 tgcggccgac acgtgagaac gtcagcagat cgttgatcag ccgctgcatc cgggaggcgc
     6661 cgtcgacggc gaacgcgatg tactgatcgg cgcggtcgtc gagcttcccg gcgtacctgc
     6721 gctgcagcag ctggcagaag ctcgacacct tgcgcagcgg ctcctgcagg tcgtggctcg
     6781 ccacgtacgc gaactgttcg aggtcgcggt tggagcgctc cagctcgcgg gcctgctcgt
     6841 cgaggcgccg attcgccgcc tgtgcgtcgt cgaggtcgcg ctggatgtgc tggcgcatcg
     6901 cctcgacgtc cgcgccgagc ccgacgatct cacgcgggcc gcccgcgcga accggttgcg
     6961 tggcatcgcc ggtgacgacc tcgcgcacct gtaccgccag atccgacaac ggccgcagca
     7021 ttccccggcg caacccgata ccggccgcga ccaggaacga cgccagcaat gcagcgatcg
     7081 ccacccccac gaccagcagg aacgccgacg ccccctcgat gctgaccctg gccgcctccc
     7141 gctccacctg taggtcggcc agcatcgtct gcaccgcact ccgcacctgc tcgaacagtg
     7201 cccgtccgcg ctcggggtcg ggcccgccag gcgttccggc gaggacgggt ccggcgtact
     7261 cggtgcgcca gtcctgtgca gcccgttcca cggcgtcgag ccggccgtcg aggccaggaa
     7321 ggcggccgcc ggcggccagc tcgcgtaggt gggcgaccat ttcggcctcg gtcgcccgcc
     7381 cgtcctggta gggctgcagg aactcggcgc gctgggccag gctgtaacca cgcacgccgg
     7441 tctcctggtc gaccagcgcg gtcgacagct gctgcgcggc gatcaacgcc ggtccggccg
     7501 agtcgaccag cgcgatcctg gcctcggcga gtcgccacag cgcgacgccg ccgaggacca
     7561 cgacgaccag tgcgaacagc gctgccagcg aggcaccgag ggcgaagatc cgcgcaagcg
     7621 gccaatgctt ccggccctcc tccaaccgct gcaggcgcgc ctccccactg cgctcgggag
     7681 gcgtccggtc gagagcggtc acggcacctc caccgcgaac ggcgccacct gggcctgggg
     7741 agcgtcctga ggcgcggtgt ccgaggagag cagcaggatt gcgacgtcgt cggacagcgg
     7801 gccgccgacg gcagccttcc cagcctcgac gcccggctcg acgccgtgga gcgggctgca
     7861 gaggactggc gtaccgagta cgccgcaccc gtcctcgccg gaacgcctgg cggctccgac
     7921 cccgagcgcg gacgggcact gttcgagcag gtgcggagtg cggtgcaggc gatgcttgcc
     7981 gacctgcagg cggagcggga ggcggccagg gtcggcatcg agggggcgtc ggcgttcctg
     8041 ctggtcgtgg gggtggcgat cgctgcatt
//
LOCUS       DASRSC010000268         2874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1416650,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000268 DASRSC010000000
VERSION     DASRSC010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416650"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VFE39_13130"
     CDS             complement(<1..676)
                     /locus_tag="VFE39_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259588.1"
                     /translation="MSPIENGGGQERNSALDTPVEKLFVGARVIDPESGLDGPLQVGV
                     RDGTITYVGSERILAESTVDVSGLVLCPGFIDMHSHAQSVNGLRLQALDGVTTSLDLE
                     SGALPVPLTYEVVAEEGRPINYGYAASWATARLHVVDDMPMPARSASGEMPASLDVFQ
                     ARQNAARWNSVASKAEVDRIIAVLEEGIADGAIGIGVLLGYAPDSGRTEYFRTAQLAS
                     RLGVGVF"
     gene            complement(673..1956)
                     /locus_tag="VFE39_13135"
     CDS             complement(673..1956)
                     /locus_tag="VFE39_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET6259589.1"
                     /translation="MTRAEDLIAGLEDRLPGLEALYRDLHEHPELSFQEKRTAAVMAG
                     RLAAAGFEVTTGVGGTGVVGVLRNGQGPTVLLRADMDALPVEEIEKVPYRSRATGTDP
                     DGLAAPVMHACGHDTHMTALVGAIEQLAARRAHWSGTVLAVLQPAEEVGGGARAMLDD
                     GFLERFPRFDVALGQHITSAPSTHLYACPGVFMAAADSLRITVHGRGGHGSTPQATID
                     PVVIAASIVLRLQTIVAREVAPTDVAVVTVAAIHAGRKENVIPAEAELKVNVRTFDPD
                     VRAHVLAAITRIVQAEAAAAGAPRKPEIAPINGFPLLTNDVETTRNVMAAFSEAFGVD
                     HVHEMTPKTGSEDFGCFAEAAGVPSVFWNFGGFDPALFPDGPQRPQVAVAAGRAPGGH
                     SPEFVPTGLEPTLRRAVEAMLTAAAIWLVPGVAAA"
     gene            complement(1943..>2874)
                     /locus_tag="VFE39_13140"
     CDS             complement(1943..>2874)
                     /locus_tag="VFE39_13140"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6259590.1"
                     /translation="AIDEAIEVGRRTGVRVEISHLKAVGPANWGRVGAAMEQIAAARR
                     DGVDVRADQYPYAAGSTTLTTRLPGWAMEGGVPALLRRLADPAMCDRIVAELEADDIW
                     SFLPPRIVVADTPEGPFRRFVGQDLATIAAAIGEPPARAMLEVLRRQRGIVSIIMHGM
                     AEDDVRLALADPTVAVASDGWVLHCVGAGMPHPRSFGTFVRVLGRYVRDEELLDLPTA
                     VAKMTSLPASRLGWSDRGVLRPGAVADVACFDPDRVCDPSTFAEPWQLAQGVVHTLLR
                     GQLVWEDGAPTGAAAGQVVRTMSEKGRSRRDPS"
BASE COUNT          409 a         1082 c          993 g          390 t
ORIGIN      
        1 tgaagacacc cacgccaagc cgcgaggcga gctgcgccgt ccggaagtac tcggtacggc
       61 cggagtcggg tgcgtagccg agcaggacgc cgatcccgat cgcgccgtcg gctatcccct
      121 cctccagcac ggcgatgatg cggtccacct cggccttcga cgcgaccgag ttccagcggg
      181 ccgcgttctg ccgggcctgg aacacgtcca gcgaggccgg catctcgccg gacgccgatc
      241 gggcgggcat cggcatgtcg tcgaccacgt ggagccgggc cgtcgcccac gacgccgcgt
      301 agccgtagtt gatgggcctg ccctcctccg cgaccacctc gtaggtgagc gggacaggca
      361 gcgctcccga ctccaggtcc agcgaggtgg tcaccccgtc gagggcctgc aggcggagcc
      421 cgttgacgga ctgggcatgg ctgtgcatgt cgatgaagcc gggacagagc acgagaccag
      481 agacgtcgac agtcgactcg gcgaggatgc gctcgctccc cacgtaggtg atcgtgccgt
      541 cgcgcacgcc cacctggagg gggccgtcca gcccggactc gggatcgatc acccgcgccc
      601 ccacgaacag cttctccacc ggggtgtcga gcgcggagtt cctctcctgc cctccgccgt
      661 tctcgatggg gctcacgcgg cggccacgcc gggcaccagc cagatcgcgg ccgccgtcag
      721 catcgcctcg acggcacgtc gcagggtcgg ctcgaggccg gtcgggacga actcgggcga
      781 gtggcccccg ggagccctcc cggcggccac cgcgacctgc ggccgctgcg ggccgtccgg
      841 gaagagcgct gggtcgaacc cgccgaagtt ccagaagacc gacggcaccc cggcggcctc
      901 ggcgaagcac ccgaagtcct cgctgcccgt cttgggggtc atctcgtgca cgtggtccac
      961 accgaacgcc tcgctgaacg ccgccatcac gttcctcgtc gtctcgacgt cgttggtgag
     1021 cagcggaaag ccgttgatcg gggcgatctc gggtttccgg ggcgctccgg cggccgcggc
     1081 ctccgcctgc acgatccgag tgatcgcagc cagcacgtgc gcccggacgt ccggatcgaa
     1141 ggtgcggacg ttgaccttga gctccgcctc cgcggggatg acgttctcct tgcggccggc
     1201 gtggatggcg gccaccgtca ccacggccac gtcggtcggc gcgacctccc gcgcgacgat
     1261 cgtctgcagc cgcagcacga tggacgcggc gatgacgacc gggtcgatgg tcgcctgcgg
     1321 cgtcgatccg tgcccaccgc gcccgtggac ggtgatccgc aggctgtcgg ccgcagccat
     1381 gaacacgccg gggcaggcgt agaggtgggt gctgggcgcg gaggtgatgt gctgccccag
     1441 cgcgacgtcg aagcgcggga accgctccag gaacccgtcg tcgagcatcg cgcgggcacc
     1501 accccccacc tcctcggccg gctggaggac ggcgagcact gtgcccgacc agtgcgcgcg
     1561 gcgcgcggcg agctgctcga tcgcgccgac gagtgcggtc atgtgggtgt cgtgcccgca
     1621 ggcgtgcatg accggtgcgg cgagcccgtc cgggtcggta ccggtggcgc ggctgcggta
     1681 gggcaccttc tcgatctcct cgaccggcag tgcgtccatg tcggcccgca gcagcacggt
     1741 cggcccctgc ccgttgcgca gcaccccgac cacgccggtc ccgcccaccc cggtcgtcac
     1801 ctcgaacccg gcggcggcga gccggcccgc catcaccgcc gcggtacgct tctcctggaa
     1861 cgacagctcg gggtgctcgt gcaggtcgcg atagagcgcc tccaggcctg gaaggcgatc
     1921 ctccaggccg gcgatcagat cctcagctcg ggtcacgacg actccgtccc ttctcactca
     1981 ttgtgcgcac cacctgcccg gcggcagcgc ccgtgggggc cccgtcctcc cacacgagct
     2041 gcccccgtag gagcgtgtgg acgacgccct gtgccagctg ccacggctcc gcgaacgtcg
     2101 acgggtcgca gacccggtcc gggtcgaagc aggcgacgtc ggcgacggcg ccgggacgca
     2161 ggacaccgcg gtcggaccag cccagccgcg acgccgggag cgaggtcatc ttcgcgaccg
     2221 ccgtcggtag atccagcagc tcctcgtcgc ggacgtaccg gcccagcacc cggacgaagg
     2281 tgccgaagct gcgagggtgt ggcattcccg cgccaacgca gtgcaggacc cacccgtcac
     2341 tcgcgacagc caccgtaggg tcggcgagcg ccagtcggac gtcgtcctcc gccatcccgt
     2401 gcatgatgat cgacacgatg ccgcgctgcc tgcgcagcac ttccagcatc gcgcgggcag
     2461 gcggttcccc gatcgccgcg gcgatcgtgg caaggtcctg gcccacgaac cggcggaacg
     2521 gcccctcggg cgtgtcggcg acgacgatgc gcgggggcag gaacgaccag atatcgtcgg
     2581 cctccagctc ggcgacgatc cggtcgcaca tcgccgggtc ggccagcctg cgcagcaggg
     2641 ccggcacgcc gccctccatg gcccaacccg gcaagcgcgt ggtgagcgtc gtgctccctg
     2701 ccgcgtaggg gtactggtcc gcacgcacgt cgaccccgtc ccgacgagct gcggcgatct
     2761 gctccatcgc ggccccgacc cgcccccagt tggccggccc caccgccttc aggtgagaga
     2821 tctcgacccg cacgcccgtg cgcctgccga cctcgatcgc ctcgtcgatc gccc
//
LOCUS       DASRSC010000269        10548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3219666,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000269 DASRSC010000000
VERSION     DASRSC010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10548
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3219666"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VFE39_13145"
     CDS             complement(<1..232)
                     /locus_tag="VFE39_13145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259591.1"
                     /translation="MARFGGGTGRVLARTALALAACAVAVAAVVIATEGGTFPGAPVP
                     TVTLAPAAAVGCETAGPYSVPDRGSIGLPAGLG"
     gene            complement(298..1338)
                     /locus_tag="VFE39_13150"
     CDS             complement(298..1338)
                     /locus_tag="VFE39_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6259592.1"
                     /translation="MISDDDPDCDWTAPGVFRCAPDVYRIPLPLPNDGLRAVNVYAVV
                     DGEGWTLVDSGWALAQAGDLLQEALAQLGAGLRDVRRFLVTHIHRDHYTQAVVLRRDF
                     GMRVAVGRGEQPALEVLNGPQRDGPAYLRRLRRAGAGELLQRLTGAWAERVDPTIWEF
                     PDDWIDDGEEIVLGVAERGQRVLQAVATPGHTQGHVVFADPVGGLLFSGDHVLPRITP
                     SIGFEPVTAPNPLADFLASLELLRTLPDAAMLPAHGPVGMRVHERVDELLAHHSKRLD
                     ATLAAVQDGRSTAHEVAAVLPWTRRERRLDELDPFNAMLATLETSVHLDVLAERGVVT
                     VGEVDGEAHYTT"
     gene            1546..1755
                     /locus_tag="VFE39_13155"
                     /pseudo
     CDS             1546..1755
                     /locus_tag="VFE39_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1979..2968
                     /locus_tag="VFE39_13160"
     CDS             1979..2968
                     /locus_tag="VFE39_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6259593.1"
                     /translation="MTTRTRSRPARLVPVGDTALRFRMVHGYRRAFRLAGSGPPVVLV
                     HGIGDSSATWEPVIPALARRHLVIAPDLLGHGSSDKPRADYSVAAYANGVRDLLGVLG
                     VERATLVGHSLGGGVAMQFAYQFPERTERLVLVGSGGAGPDVTPVLRAMTLPGAAGLL
                     HLLRLPTARLQVGVAVQLLRLLGTGFGQDAPDLLRMVDALPDATARAAFIRTLRAVVD
                     WRGQVVTMLDRCYLTRGMPTLLVWGSRDAVVPLAHAYRAHLAMPGSRLEVFDGAGHFP
                     FHSDPARFVTLVEDFLESTEPARWSAEQWRKLLRDPEPMGIGPIHASGERNAT"
     gene            3056..3451
                     /locus_tag="VFE39_13165"
     CDS             3056..3451
                     /locus_tag="VFE39_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6259594.1"
                     /translation="MLDHLSIQASDVDAAAAFYLRVFAPLGIREAIRIDHDGAPVVGF
                     SGPDGFPHFWLGPATGSAGREVHIAFTAPSRTAVDDVYLAAVEAGAEVQHSPRVWPEY
                     HPGYYGVFVRDLDGNNVEAVHHTFPGATT"
     gene            complement(3499..6339)
                     /locus_tag="VFE39_13170"
     CDS             complement(3499..6339)
                     /locus_tag="VFE39_13170"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005440080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HET6259595.1"
                     /translation="MTETVGTTSGRGKKASGKSGARAGLTVERIYTTPGVHPYDEVTW
                     ERRDVVMTNWRDGTVNFEQRGVEFPDFWSVNATNIVTSKYFRGAPGGPGRESSLRQLI
                     DRVVGVYHRAGLDNGYFASADDAEIFAHELTWMLLHQVFSFNSPVWFNVGTSSPNQVS
                     ACFILAVDDTMESILNWYREEGLIFKGGSGAGLNLSRIRSSKELLSSGGTASGPVSFM
                     RGADASAGTIKSGGATRRAAKMVVLDVDHPDIEEFVLTKAKEEAKVRVLRDAGFDMDL
                     GGSDITSVQYQNANNSVRVTDEFMRAVEADTEFGLRSRMTGEVIDKVGAKKLFRTIAQ
                     AAWECADPGVQYDGVINDWHTCPESGRISASNPCSEYMHLDNSSCNLASLNLLTFLGD
                     DGQFDAARFQKVVEMVITAMDISICFADFPTEPIAETTRRFRQLGIGYANLGALLMAT
                     GHAYDSDGGRSLAGAITSLMTGAAYKRSAELAGVVGPYDGYARNAESHQRVMRKHAAA
                     NDDVRTLPASSGVHKLATIAWKDCLAIGARNGWRNAQASVLAPTGTIGLMMDCDTTGI
                     EPDLALVKFKKLVGGGSMQIVNQTVPRALRHLGYPAEQVEAIVEYVAEHGHVIDAPGL
                     RPDHYEVFDCAMGERVIAAMGHVRMMAAVQPFLSGAISKTVNMPETATVEDVEKIYLD
                     GWKLGLKALAIYRDNCKVGQPLSAGKSAKATEKVEAAPVEARPVRRRLPKKRPSETVS
                     FTVGGAEGYLTAGSYPDNGLGEIFVKLGKQGSTLAGVMDAFSMSISVGLQYGIPLEFY
                     VSKFSNLRFEPAGMTDDPDVRIATSVLDYLFRRLALDHLPYEKRAQLGIFSADERTRQ
                     VAGSDYSTGYGEVDVEGLRSSVDAAPVAGEVERTPGAAPVPVEVGSSTELLELRLGKA
                     ADAPLCMTCGTKMRPAGSCYLCEGCGSTSGCS"
     gene            complement(6470..6931)
                     /gene="nrdR"
                     /locus_tag="VFE39_13175"
     CDS             complement(6470..6931)
                     /gene="nrdR"
                     /locus_tag="VFE39_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HET6259596.1"
                     /translation="MRCPFCRHSDCRVIDSREVDDGQATRRRRSCAACGRRFTTVEEA
                     VLAVVKRSGVTEPFSREKVVSGVRRACQGRPVDEDALQQLAHRVEEAVRAGGTAEIPS
                     HEVGLAILGPLRELDEVAYLRFASVYRSFSSIEDFEKEIAELRSPGDAPAD"
     gene            complement(7111..7446)
                     /locus_tag="VFE39_13180"
     CDS             complement(7111..7446)
                     /locus_tag="VFE39_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259597.1"
                     /translation="MALPVPAPWVRARRVLAAAALTVVAATVVVGLGLLAGATAESRA
                     VVGGSSDAEVMTTVTVGPEATVWDLARTVAPAASGPELAAVTDRIVTDNSLTSVRLHR
                     GQVLRVSVS"
     gene            8171..8965
                     /gene="lexA"
                     /locus_tag="VFE39_13185"
     CDS             8171..8965
                     /gene="lexA"
                     /locus_tag="VFE39_13185"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HET6259598.1"
                     /translation="MARDEPGAAAGSEAGPVTPDGGTGSRNGRSAQVSTFPDPPTGPA
                     GLTPRQRKVLEVIRDWVERFGYPPSVREIGDAVGLTSTSSVHHQLRTLERKGYLRRDP
                     NRTRAVDVRGPDDPAAVDDTAAPGAIAVEDTTADDLRPAPAFVPLLGNIAAGGPILAE
                     QAVESVFPLPREIVGEGTLFLLNVRGDSMIEAAITDGDWVVVRQQPVAENGEIVAAMI
                     DGEATVKTLKRRDGHIWLMPANPAYDPIPGDEASVLGRVVAVLRRL"
     gene            complement(9002..9934)
                     /locus_tag="VFE39_13190"
     CDS             complement(9002..9934)
                     /locus_tag="VFE39_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259599.1"
                     /translation="MPAPEARCAVDDPRLTELSGLVVDGGALWAMSDGGSRVELHRLD
                     PSTCAVIDSRTAAVNPYDAEDLALAPDGALWVADIGDNERRRDTVAMIVLPVRGESRL
                     HRLTYPDGPHDAEALLVDTAGRPVVITKEARSPAGVYRTVQPPEGLGPTPLVRVGELT
                     LPPSATQGGPLGVMGSRMVTGAAATADGSVVAVRSYTDAWLYPVRGGDLVAALEATPV
                     QVPLPDEPQGEAVAFESDGTLVSGSETRGVLGEIRAVPEAAGLVADRLPAAAPSTAAP
                     TDEPAPDWLPAGIGAAVVVGALLLVIAAMAVRRR"
     gene            complement(9979..>10548)
                     /locus_tag="VFE39_13195"
     CDS             complement(9979..>10548)
                     /locus_tag="VFE39_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET6259600.1"
                     /translation="DGRTYTLTDTVGFVRHLPHQLVEAFRSTLEETAGADLLVHVVDA
                     SDPLPEDQIKAVRQVLVEIGEQNAGAMPQELLVVNKTDAADDLQLARLRHLLPDAVFV
                     SARRGDGVQKLRERIAELMPRPEVDVELLLPYAEGSLVARVHSEGEVLVEEHTPEGTR
                     MHARVGAELATAVLPFALVGMTAEDGAGR"
BASE COUNT         1549 a         3816 c         3680 g         1503 t
ORIGIN      
        1 cgccgagtcc ggccggaagg ccgatgctgc cgcggtcggg gacgctgtag gggcccgcgg
       61 tctcgcaacc gacggcggcc gccggggcga gggtcacggt gggaacgggg gcgccgggaa
      121 acgtaccgcc ctcggtggcg atgacgacgg cggccacggc gactgcgcag gccgcgaggg
      181 cgagcgcagt acgcgcgagg acccgccccg tcccaccacc gaacctcgcc acggcggaga
      241 gcgtacggcc ggtgtcctgg gctgtcgccg gtgggctcta cgaagagtga attgctatca
      301 cgttgtgtag tgagcttcgc cgtccacctc gccgaccgtc acgactcccc gctcggccag
      361 cacatccagg tgaacagaag tctccagcgt cgcgagcatc gcgttgaacg ggtcgagctc
      421 gtccaggcgc cgttctcgac gcgtccacgg cagcacggcc gccacctcgt gggcggtgga
      481 gcggccgtcc tggacggcgg ccagtgtcgc gtcgagccgc ttgctgtggt gggccagcag
      541 ctcgtcgaca cgttcgtgca cgcgcatccc caccggaccg tgggccggga gcatcgccgc
      601 atcgggcagt gtccgcagga gctccagcga ggcgaggaag tcggccagcg ggttgggagc
      661 agtcaccggc tcgaatccga tcgagggggt gatccggggc agcacgtggt cgccggagaa
      721 cagcaggccg cccaccggat cggcgaagac gacatggccc tgggtgtggc cgggggtcgc
      781 gaccgcctgc agcacccgct gcccgcgctc cgccacgccc aggacgatct cctcgccgtc
      841 gtcgatccag tcgtcgggga actcccatat cgtcgggtcg acgcgctcgg cccacgcgcc
      901 ggtgaggcgc tggagcagtt cgccggcgcc cgcgcgacgc aaccgccgca ggtacgcagg
      961 tccgtcccgc tgcggaccgt tgagcacctc cagtgcgggc tgctcgccgc ggccgactgc
     1021 gacccgcata ccgaagtccc tgcggagcac gacggcctgg gtgtaatggt cacggtggat
     1081 atgggtgacg agaaaccggc gcacatcgcg caggccggca ccgagctggg ccagcgcctc
     1141 ctgcagcagg tcgccggcct gggcgagcgc ccatcccgag tcgacgagcg tccacccctc
     1201 gccgtcgacc accgcataca cgttcaccgc ccgcagcccg tcgttcggga gtggcaacgg
     1261 aatgcggtag acgtcgggcg cacaccggaa cactcccgga gcggtccagt cacagtccgg
     1321 atcgtcgtcg gatatcacag cccgacccta ggcggggccg gggcgatcca tccactcagc
     1381 aagggtcacc gactacacac gctgcgtagt cgacgggcgc cggaccgcgg gacaccaccc
     1441 gtcggtgcac ctcgacatac ccgaaagtgt ggcctcaggt ccgttactgt ccacctcaga
     1501 cggagatcaa ctaccccgtt cggggtgacc gaggaggacg tgcacatggc tgctgacggg
     1561 atccgacccc cagacatgat cggggcgccg ttcctcaccg tggccgaggt cgcccagcgg
     1621 atgcgtgtct cgaagatgac cgtctaccgg ctcgtccaca gcggagaact gccggccgtg
     1681 cgcttcggcc gttcgttccg cgtgcagcgt gccgcggtgg aggagctgat ccgggcggcc
     1741 cgatacgagg ccggccgagc cgtcctcaac gcccaccccg acgccgcggc ggcgacgggc
     1801 ggagcacagg cacgctgagc cccgctgcgg gccggtacgc cgtcgtgatc cccctctggc
     1861 cccgatcacc gcatccgccg ccaggtcaca cgaacctcca gctcgacgtc tcctcatcgg
     1921 cctgagccga gcagccgcaa cactgcggta accactgcga cctaccctgg cggtggtcat
     1981 gaccacgcgc accagatctc gcccggcgcg gctcgtcccg gtcggtgaca ccgcgctgcg
     2041 cttccggatg gtccacggct accgccgggc cttccggctg gccggttcgg gtccgccggt
     2101 ggtgctcgtg cacgggatcg gcgacagctc cgccacctgg gagcctgtca tccccgccct
     2161 ggcccgccgg cacctggtga tcgcccccga cctgctcggc cacggcagtt ccgacaaacc
     2221 gcgggccgac tactccgtgg ccgcctacgc aaacggcgtg cgcgacctgc tcggggtgct
     2281 cggcgtggag cgggcgacgc tcgtggggca ctcgctcggc ggcggggtgg cgatgcagtt
     2341 cgcctaccag ttcccggaac ggaccgagcg cctcgtgctg gtgggcagcg gcggcgccgg
     2401 cccggacgtc acgccggtgc tgcgtgcaat gacgctgccc ggtgcagccg ggctgctgca
     2461 cctgctgcgg ctgccgacgg ccaggctgca ggtcggggtg gccgtgcagc tgttgcggct
     2521 gctcggcacc ggcttcggcc aggacgcccc cgacctgctc cgcatggtcg acgcgcttcc
     2581 cgacgccacg gcgcgcgccg cgttcatccg cacgctgcgg gccgtggtcg attggcgcgg
     2641 tcaagtggtg acgatgctcg accgctgcta cctcacccgc ggcatgccca cgcttctcgt
     2701 ctggggatcg cgcgatgccg tggtgccgct ggcgcatgcc taccgtgcgc acctggccat
     2761 gccgggcagc cgcctggagg tgttcgacgg cgccggccac ttcccgttcc acagcgatcc
     2821 ggcgcgcttc gtcacgctcg tcgaggactt cctcgaatcg acggagcctg cacggtggag
     2881 cgccgagcag tggcggaagc tgctgcgcga cccggagccg atgggtatcg gaccgatcca
     2941 cgcttctggc gagcgcaacg cgacctgact ctccgaccgg gatcgtcggt gtccctgcct
     3001 accgtgaatc cacccacgga cccgtggccc gttccccgca ctcccggagg ccccgatgct
     3061 ggaccacctc tcgatccagg cgtccgacgt cgacgccgct gccgccttct acctgcgcgt
     3121 gttcgccccg ctgggcatcc gcgaggcgat ccgcatcgat cacgacggcg cgccggtggt
     3181 cggcttctcc ggtccggacg ggttcccgca cttctggctc gggcccgcca ccggttcggc
     3241 tggccgggag gtgcacatcg cgttcaccgc acccagccgg accgccgtcg acgatgtgta
     3301 ccttgcggca gtcgaggccg gagcggaggt ccagcactcc cccagggtgt ggccggagta
     3361 ccaccccggt tactacgggg tcttcgtccg ggacctcgac ggcaacaacg tggaggccgt
     3421 ccaccacacc ttcccgggag ccaccacctg acggcgtacc ccggaaccgc acaccacggc
     3481 tccgaagtac gccacgtgtc agctgcagcc gctggtggag ccgcagccct cgcacaggta
     3541 gcaggaaccc gccggccgca tcttcgtgcc gcacgtcatg cacagcggcg cgtcggcggc
     3601 cttaccgagg cgcagctcca gcagctcggt ggagctgccg acctcgaccg ggacgggcgc
     3661 cgcgcccgga gtgcgctcca cctcgccggc cacgggcgcg gcgtcgaccg aggaccgcag
     3721 gccctccacg tcgacctcac cgtagccggt ggagtagtcg ctgccggcca cctgcctggt
     3781 gcgctcgtcc gcggagaaga tgcccagctg ggcgcgcttc tcgtagggca ggtggtccag
     3841 ggcgagccgg cggaacaggt agtccagcac gctggtggcg atgcgcacgt cggggtcgtc
     3901 ggtcataccg gcgggctcga agcgcaggtt ggagaacttc gagacgtaga actccagcgg
     3961 gatgccgtac tgcaggccta cggagatcga catggagaac gcgtccatca cgccggccag
     4021 cgtcgagccc tgcttgccga gcttgacgaa gatctcaccg aggccgttgt ccgggtagga
     4081 gcccgccgtc aggtagccct ccgccccgcc gaccgtgaag gacacggtct cgctcgggcg
     4141 cttcttgggc agcctgcgcc gcaccggcct cgcctccacg ggcgcggcct ccaccttctc
     4201 cgtggccttg gccgacttgc ctgccgacag cggctggccg accttgcagt tgtcgcggta
     4261 gatcgccagc gccttgaggc cgagcttcca gccgtccagg tagatcttct cgacgtcctc
     4321 gacggtggcc gtctcgggca tgttgaccgt cttggagatc gcaccggaga ggaacggctg
     4381 gacggcagcc atcatccgca cgtggcccat ggcggcgatg acccgctcgc ccatggcgca
     4441 gtcgaacacc tcgtagtggt cgggccgcag gcccggcgcg tcgatgacat ggccgtgctc
     4501 ggcgacgtac tccacgatcg cctcgacctg ctcggccggg tacccgaggt ggcggagcgc
     4561 ccgcggcacc gtctggttga cgatctgcat ggagccgccg ccgaccagct tcttgaactt
     4621 gaccagcgcc aggtcgggct cgatgccggt ggtgtcgcag tccatcatca ggccgatggt
     4681 gccggtgggg gcgagcaccg atgcctgcgc gttgcgccag ccgttgcgcg ccccgatcgc
     4741 gaggcagtcc ttccacgcga tcgtggcgag cttgtggacc ccgctgctcg ccgggagcgt
     4801 gcgcacgtcg tcgttggccg cggcgtgctt gcgcatgacc cgctggtgcg actccgcgtt
     4861 gcgggcgtag ccgtcatagg gaccgacgac gccggcgagc tccgccgacc gcttgtaggc
     4921 cgcccccgtc atcaacgagg tgatggcgcc ggcgagtgac cgccctccgt cggagtcgta
     4981 cgcatggcca gtggccatga gcagggcgcc gaggttggcg tagccgatac cgagctggcg
     5041 gaaccggcgg gtggtctccg cgatcggttc ggtggggaag tcggcgaagc agatggagat
     5101 gtccatcgcc gtgatcacca tctcgacgac cttctggaac cgcgccgcgt cgaactggcc
     5161 gtcgtcgccg aggaacgtga gcaggttcag cgaggcgagg ttgcagctgg agttgtccag
     5221 gtgcatgtac tccgagcacg ggttggacgc ggagatccgg cccgactcgg gacaggtgtg
     5281 ccagtcgttg atgacgccgt cgtactggac gccggggtcg gcgcactccc atgcggcctg
     5341 ggcgatggtg cggaagagct tcttcgcccc caccttgtcg atgacctcac ccgtcatccg
     5401 ggaccggagg ccgaactcgg tgtcggcctc gaccgcccgc atgaactcgt cggtgacgcg
     5461 caccgagttg ttggcgttct ggtactgcac cgaggtgatg tcggagccac cgaggtccat
     5521 gtcgaacccg gcatcgcgca gcacccggac cttggcctcc tccttcgcct tggtgagcac
     5581 gaactcctcg atgtccgggt ggtcgacgtc gaggacgacc atcttcgccg cgcgccgcgt
     5641 ggcgcccccg gacttgatgg tgcccgcgct tgcgtcggca ccccgcatga aggagaccgg
     5701 cccggacgcc gtgccaccgg aggacagcag ctccttcgac gagcggatgc gggagaggtt
     5761 gaggcccgcg ccggagccgc ccttgaagat cagcccctcc tcgcggtacc agttgaggat
     5821 cgactccatg gtgtcgtcga cggcgaggat gaaacacgcc gagacttgat tcgggctcga
     5881 cgtgccgacg ttgaaccaca ccggggagtt gaagctgaag acctggtgca gcagcatcca
     5941 cgtgagctcg tgggcgaaga tctcggcgtc gtcggcgctg gcgaagtagc cgttgtcgag
     6001 gcccgcgcgg tggtagaccc cgacgacccg gtcaatgagc tggcgcaggc tcgactcgcg
     6061 ccccggaccg ccgggggcgc cgcggaagta cttgctggtg acgatgttgg tggcgttgac
     6121 cgaccagaag tcggggaact cgacgccgcg ctgctcgaag ttgaccgtgc cgtcgcgcca
     6181 gttggtcatg acgacgtcgc ggcgctccca ggtgacctcg tcgtacgggt ggacaccggg
     6241 ggtggtgtag atccgctcga cggtgaggcc ggcccgcgcg cccgacttcc cggacgcctt
     6301 cttccctcgt cctgacgtgg ttccgacggt ctcggtcatg ctctccctct cgatccggtg
     6361 ccggcggctg ggtccgtcgg tacgcggtcc tggctgcgct tctgctgctg ttcgggggta
     6421 ttcctgggtg gaacgagccg gcgacgacgc cggccggtgc ggatcgggct cagtcggccg
     6481 gagcgtcgcc gggactgcgg agctcggcta tctccttctc gaagtcctcg atggaggaga
     6541 acgagcggta gacactcgcg aaacggaggt aggcgacctc gtcgagctcc cgaagcgggc
     6601 cgaggatggc cagcccgacc tcgtgactgg ggatctcggc ggtgccgcct gccctgacgg
     6661 cctcttcgac acggtgggcg agctgctgca gcgcgtcctc gtcgacaggg cggccctggc
     6721 aggcccgacg aacgcccgag acgaccttct cgcgactgaa cggctcggtg accccgctgc
     6781 gcttgacgac agcgagcacg gcctcctcga cggtggtgaa ccggcgaccg cacgcggcgc
     6841 acgaacgcct gcgccgtgtg gcctggccgt cgtcgacctc ccgggagtcg atcacccggc
     6901 agtcggagtg tcggcagaac gggcagcgca tcggaccgat cccttcaccg aacgttctcg
     6961 atttcgccgt ccgggaaccc cactcctgga cgaacaacac gagtgtaacc acaagatgta
     7021 gggccagggg aagccggagg ccccatcagt gccggtgttc gtccgatcgg gcacgggcgc
     7081 catccggcgc gtcgcgccgg atgccccctg tcagctcacg ctcacccgca gcacctgccc
     7141 gcggtgaagc cgcaccgatg tgagcgaatt gtcggtgacg atccgatcgg tgaccgcggc
     7201 cagctcggga cccgacgcgg caggcgcgac ggtgcgagcg aggtcccaga cggtggcctc
     7261 cggacccacg gtgacggtgg tcatcacctc cgcgtccgac gaaccgccca cgaccgcccg
     7321 cgactcggcg gtggccccgg ccagcagccc gagaccgacc accacggtcg cggccacgac
     7381 ggtgagggcc gccgccgcga gcacgcggcg cgcccgaacc cacggcgcgg gcacggggag
     7441 ggccacgatg gatcgccgga ccagcagtcg ttgacccgag gcctccggca ctgcccgcac
     7501 cacgggacgc ggcagttgag cgggccgcac gaccaccggc accacggccg ggcgcaccgg
     7561 gcgcggcccc cgcggccggc gtgggcctac ccacccgggc cgaccgctcc cacggccgac
     7621 agacggactg cccgacagcc ccacgacacc gccgggctcg tcgagggcga cgcgctcggc
     7681 cgccggcgcg acgcccgacc ccctccggcc ggagctcgcc tggcgccgct tcccgcgatc
     7741 cgccgccgcg tgccgtcgct ctccacctgg ttgatcctcg gccggccgtc cgatctgctc
     7801 gtgctcgtcc atcgccatcc gcctctcgcc gctccccgcg cgactcaaac aaccgttcga
     7861 acgatcgcct gtgggatgtg taccaccggg ggccgacaga atcgagaaaa ctgggcgtgt
     7921 cgttcgaaca tgcgtttggc gaagaacggg atcggggcta ccgtcgtgcc gacggcggtg
     7981 gccccccgtc aacggctcgg gcaaccgggt gagcacccgc accatcggca ccgaccagcg
     8041 gcgccgcacc accgcatgac gagcgacaca gcgcagcacg caggacgaac cacgaacagc
     8101 acgtgagctc caccggcacc accatcgccg gcggcgacca tcgagggcga ccatctagga
     8161 ggcacgacga atggcacggg acgagcccgg cgccgcggcc ggtagcgagg ccggcccggt
     8221 cacacccgac ggcggcacag ggtcgcgtaa cggcaggtcg gcgcaggtca gcaccttccc
     8281 cgaccctccg acggggccgg ccgggctgac accgcggcag cgcaaggtcc tcgaggtgat
     8341 ccgcgactgg gtcgagcggt tcggctatcc gccgagcgtg cgcgagatcg gtgacgccgt
     8401 cggactcacc tcgacgtcct ccgtgcacca tcagctgcgc accctggagc gcaagggcta
     8461 cctgcgccgg gatccgaacc ggacgcgtgc cgtcgacgta cgggggcccg atgaccctgc
     8521 tgcggtcgac gacaccgcgg cgcccggggc gatcgcggtc gaggacacca cagccgacga
     8581 ccttcgtccg gcgccggcgt tcgtgccgct gctcggcaac atcgccgccg gtggaccgat
     8641 cctcgccgag caggccgtcg agagcgtgtt cccgctgccc cgcgagatcg tcggagaggg
     8701 caccctcttc ctgctgaacg tccgcggtga ctcgatgatc gaggccgcga tcaccgacgg
     8761 tgactgggtt gtcgtccggc agcagccggt cgcggagaac ggcgagatcg tggcagcgat
     8821 gatcgacggc gaggccacgg tcaagacgct caagcggcgc gacgggcaca tctggttgat
     8881 gccggccaac ccggcctacg acccgatccc cggcgacgag gccagcgtgc tcggccgcgt
     8941 agtggcggtg ctgcgcaggc tctgaccacg ggtacgcgct gcgggaacgt cgccggatcg
     9001 ctcagcgacg gcgcacggcc atcgccgcga tcacgagcag cagtgccccg acgacgacgg
     9061 ccgcgccgat cccggccggc agccagtcgg gtgcgggctc gtccgtcgga gcggccgtcg
     9121 acggtgcggc cgccggaagc cggtccgcca cgagtcccgc cgcttccggc accgcacgga
     9181 tctcaccgag cacgccgcgg gtctccgacc cggacacgag cgtcccgtcg gactcgaacg
     9241 ccaccgcctc gccctgcggc tcgtccggca gtggcacctg cacgggcgtg gcctcgagcg
     9301 cggccacgag gtcaccgccc cgcacggggt agagccaggc atcggtgtag ctgcgcaccg
     9361 ccacgaccga gccgtcggca gtcgcggcgg caccggtcac cattcgcgat cccatcacac
     9421 cgaggggtcc gccctgcgtg gccgacggcg gcagcgtcag ctcgccgact cgcaccaggg
     9481 gcgtgggccc gagtccctcg ggtggttgca cggtgcggta caccccggcg ggactccggg
     9541 cctccttcgt gatcacgacc ggtcgaccgg ccgtgtccac cagcagagct tcggcgtcgt
     9601 gcgggccgtc ggggtacgtc agccggtgca accgtgactc cccgcgcacc ggtagcacga
     9661 tcatggccac ggtgtcccga cgccgttcgt tgtcgccgat gtcggccacc cacagggctc
     9721 cgtcgggagc gagggccaga tcctcggcgt cgtacgggtt cacggctgcg gttcgcgagt
     9781 cgatgaccgc acacgtggac gggtccagcc gatgtagctc cacccgggat ccgccgtcgc
     9841 tcatcgccca cagcgcgccg ccgtcgacga cgagcccgga cagctccgtg agcctggggt
     9901 cgtcgacggc acaccgggct tccggagcag gcacgccggc gagcgacgct acgagcgcac
     9961 cggccaggag gagctggatc agcgtccggc gccgtcctcg gcggtcatac cgaccagcgc
    10021 gaacggcagc acggcggtgg cgagctccgc gccgacgcgg gcgtgcatcc gggtgccctc
    10081 gggggtgtgt tcttccacca gtacctcacc ctcggagtgc acgcgcgcca ccagcgaacc
    10141 ctcggcgtag ggcagcagca gctcgacgtc gacctcgggc cgcggcatca gctcggcgat
    10201 gcgctcgcgg agcttctgga ccccgtcgcc acgccgtgcg gagacgaaca ccgcatccgg
    10261 cagcaggtgg cgcagccgcg ccagctgcag atcgtccgcg gcgtcggtct tgttgacgac
    10321 gagaagctcc tgcggcatcg cgcccgcgtt ctgctcgccg atctcgacga gcacctggcg
    10381 gacggccttg atctggtcct ccggaagcgg gtcggaggcg tcgacgacgt gcacgagcag
    10441 gtcggcgccc gccgtctcct ccagcgtgga ccggaacgcc tcgacgagct ggtgcggcag
    10501 gtgccgcacg aacccgaccg tgtcggtcag ggtgtaggtg cgcccgtc
//
LOCUS       DASRSC010000270        15263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1744162,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000270 DASRSC010000000
VERSION     DASRSC010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 15263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15263
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744162"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..121
                     /locus_tag="VFE39_13200"
     CDS             <1..121
                     /locus_tag="VFE39_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6259601.1"
                     /translation="RRRQCSLASLTSLVFTGTELTQLRYSEPAGASEPGQTGA"
     gene            118..693
                     /locus_tag="VFE39_13205"
     CDS             118..693
                     /locus_tag="VFE39_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HET6259602.1"
                     /translation="MRRIAIAIGVAAVLLLAGCSTSKDAVAQTGNFEFVAPGGQTTIT
                     YDPPASRGRVNGITGESLLHDGQTVGLENYPGKVVVLNIWGSWCGPCRTEAPELQSVQ
                     DQTGAVVLGIDVRDDRPAATDFVRDRSITFDSIFDAPGRTLAALRGYPRNTVPSTLVL
                     DRQHRVAAVYLTTIRVSELVPLIQRLQAEPA"
     gene            801..1577
                     /locus_tag="VFE39_13210"
     CDS             801..1577
                     /locus_tag="VFE39_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HET6259603.1"
                     /translation="MDPTTTLAVSGPLLLAVAVAVLAGAVSFASPCVVPLVPGYLAYL
                     AGLVGADAPPATLAQAREGRTGRWRVAGAAVLFVAGFTVVFGAILVGVVWLSDALVAN
                     EAALQRIGGVVMIAMGLAFVGLIPVLQNERRVHWVPRAGVWGAPLLGAVFGLGWVPCI
                     GPTLAGVVAVAAGTGGGSLRGVVLTVAYCAGLGLPFVLIALGASRAVRALGWLRRHTR
                     AIQIAGGVLMVGVGFLLVSGLWGGLLAQLQTSVAGFTPVL"
     gene            1685..3226
                     /locus_tag="VFE39_13215"
     CDS             1685..3226
                     /locus_tag="VFE39_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HET6259604.1"
                     /translation="MRTALILLFLLAVAALPGALLPQRSLNQRLVDQYFTDYPAIAPV
                     LDRLGFFDVFAAPWFAAIYLLLMVSLVGCVLPRTFEHAKNLRAAPVVTPRNFARLPHH
                     ATATLDVGPDEAAERIRGRLRRWRRLETEAGSSRTISAEKGYLREAGNLLFHLSLIGL
                     LVGFGAGKLYGYGGQVIVLADGGQFCNTGILGYDSFQAGLRVDGTDLTPFCLRVDDFE
                     ATYLPDGQPESYRAAIGYQTTDDLNAGRVDQWRPYPLAVNSPLRLDGSRVYLINHGYA
                     PRFTVTFPNGQKRTGEVQWQPVDATTYLSQGATKFEPPGITDEQKRRTSQLAITGLLA
                     PTSSGGKVVTSVYPALRDPEVAVDVLRGDLGLDDGRGQSIFQVDQSKIDSGQLVRVAR
                     ANLVPGQSITLDDGTIITFDDVRQWIALQISHDPGQDVVLVFAVLMLVGLGLSLSIRR
                     RRFWARLTPTEDGTRTVVEIGGLARTDRAGYGEEFDRLRAHLLNISDTDGSDVDGPIT
                     AAKDG"
     gene            3226..4155
                     /gene="ccsB"
                     /locus_tag="VFE39_13220"
     CDS             3226..4155
                     /gene="ccsB"
                     /locus_tag="VFE39_13220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HET6259605.1"
                     /translation="MLTELALYSDRLFMGAVGVFVFAMVLHGAEYAVHRSAKAVLVGA
                     GGPALDEHESHPEGTPVRSRSERLGRMGVALVVLGAVLLAASIVTRGLAAQRWPLGNM
                     YEFTSAICLAAVVAWLVVLRRSPASRPVGLFVLLPVIILLFLGGTVLYAPAAPVMPAL
                     QSYWLVVHVTAVTIASGLLMVPGVASLLFLLRRSGRLTGVADRLPSADVLDRLAYRLT
                     IIAFPLYTFAIIAGAIWAEAAWGRFWGWDPKETVAFVAWVIYAAYLHARATAGWRSGP
                     AAWVNVAGFAAVIFNLFFINMVVAGLHSYAGLG"
     gene            4335..5720
                     /locus_tag="VFE39_13225"
     CDS             4335..5720
                     /locus_tag="VFE39_13225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6259606.1"
                     /translation="MRERWGAIPAREPQPGATPPEPRSAEPEVEVEPALPGPAPDVSS
                     IASPGTPAVPPAWPSHPVQDGRLRGAGGGGPVAGTPTDRPEPSGSRADVPPPAAYPPV
                     TGPAAAPMPHRPGRVAIGPERIGPRTPPRNARWIDPAAVAAQEAQRARSGSEQVATDL
                     SSARLLRPTARPPQSGWRRLVYVLSGRLINPGESPADIRRRELTARINQPLLGCYKIA
                     MLSLKGGVGKTTMTATLGATFASLRGDRVVAVDANPDRGTLSQKIPLETSATVRHLLR
                     DAQRVRRYTDVRAYTSQGPSRLEVLASEQDPAVSEAFSEDDYRRTVTLLEHFYNIVLT
                     DCGTGLMHSAMYGVLGVADQLIIVSSGSIDGARSASATMDWLDAHGHGDLVRNSVAVI
                     NCVHRSGGVDLNRVAEHFAARCRSVVRIPFDAHLEEGAEVDLDRLEPATRLALLKLAA
                     AVADAFDGAVV"
     gene            complement(5766..5978)
                     /locus_tag="VFE39_13230"
     CDS             complement(5766..5978)
                     /locus_tag="VFE39_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259607.1"
                     /translation="MLFLIAFVGAAIIALVLWKAMSAGGPQVPQPPRRPNTGSGRPRV
                     SGPDDDPDFLRQLDEKVKRRDDPPGA"
     gene            complement(6082..6306)
                     /locus_tag="VFE39_13235"
     CDS             complement(6082..6306)
                     /locus_tag="VFE39_13235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BldC family transcriptional regulator"
                     /protein_id="HET6259608.1"
                     /translation="MTVTTQASSNERLLTPGEVAALFRVDPKTVTRWASAGRIGSIRT
                     PGGHRRFRESEVRSMLAELTSEATLPPSRP"
     gene            6463..6813
                     /locus_tag="VFE39_13240"
     CDS             6463..6813
                     /locus_tag="VFE39_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4229 domain-containing protein"
                     /protein_id="HET6259609.1"
                     /translation="MTSPPTGQPATGQGPGLAATVGLYALARLALVAVVTVLMLLAGT
                     PFLIAVLVGLIVALPLSMVLFRGLRSRLDSALAESRSRRSAERSALRARLRGEGVAAD
                     SGELAQRQPDPGEH"
     gene            complement(6769..7650)
                     /locus_tag="VFE39_13245"
     CDS             complement(6769..7650)
                     /locus_tag="VFE39_13245"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HET6259610.1"
                     /translation="MATIAQWIEGARPRTLPTAVSPVLAGTGAAIGYGVVAPGRALLA
                     LVVAVALVVGVNYANDYSDGIRGTDDERVGPLRLVGSKVAEPATVRTFAIGCFSIAGL
                     AGLTLVSLSGQWWLIAVGALCIAGARFYTGGRRPYGYSGLGEIAVFVFFGPVAVLGTV
                     ITQSGTPGALSVVTAVGVGLLTCAVLVANNLRDIPTDTVVGKRTLAVMLGDAETRRLY
                     AALVTVPILLSALGGLRSLPMLLGLLVLPLAALLARQVLGGVDGRRLIRVLAQTGMLL
                     LAWSVLTGIGLALSQFT"
     gene            complement(7660..8925)
                     /locus_tag="VFE39_13250"
     CDS             complement(7660..8925)
                     /locus_tag="VFE39_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259611.1"
                     /translation="MRLGTATASLALVGALSVAVATPESADAASLASWSPDLGAGEAA
                     GVTLADGTARLAAAGAYLARLGEGGPIGAALVPTGLLTLLPQQLDITTDRIVATVLGD
                     VPAGSAAAVDIRGRRSTGGWTEWIPAGPADGAPGTASMVAELPEPTSEVQGRLVLTGP
                     HGGGPAVHGLTLTAQPTTKQLTEGGPEQAALSYRVFATREGLVGGTTANGHVITERDH
                     FVALPSRRGLSPRDTSDYSVKVCAPNGRCAFAPVWDVGPWNTRDDYWNPAPTRQEWHD
                     LPQGLPEAQAAYQNDYNSGKDQFNRKVLNPAGIDLADGLFWDALGLNDNAWVTVDYLW
                     TGSERLSTVAGDLPVLAAPVAGAEVVGIAANHAAVPVQCVLGAGADRWLRIGVDQYLA
                     AAAVPGFDAAPSCPTGTGGAPANTGSTAG"
     gene            complement(9044..10207)
                     /gene="menE"
                     /locus_tag="VFE39_13255"
     CDS             complement(9044..10207)
                     /gene="menE"
                     /locus_tag="VFE39_13255"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HET6259612.1"
                     /translation="MTGVRMVQVDGSPPAVAELAGALREALDGGPAVLPFPPRGSAPT
                     AAAGPGAALVIATSGSTGVPKYVVLSATALRASALATVERLEGPARWLLALPAEHVAG
                     AQVVVRSLLAGAAPMVQDLRDGFRPDDFATATAQLGDGRRCTSLVPTQLARLLDAGGT
                     ALDALRGYAAVLVGGAALDPWLHARALAAGVRVVTTYGMSETAGGCVYDGVPLHGVRV
                     DIDETGRIRLGGPTLAEGYLGDPAGTAAAFGDGWFRTGDLGRWRGGRLEVLGRADDMI
                     ITGGEKVAPAAVEQVLAAQAGVRAACVVGLPDVEWGQVVAAAVVADPGDGPQEPDAVR
                     GGRLRAAVLAELGRAAVPRVLHTVPEIPLRGIGKPDRRAIADLLDARQHHPQE"
     gene            complement(10257..11180)
                     /locus_tag="VFE39_13260"
     CDS             complement(10257..11180)
                     /locus_tag="VFE39_13260"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003081878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HET6259613.1"
                     /translation="METAAPSELFDPDAWRVVDGFDFTDITYHRATAAGPAGGTVRIA
                     FDRPEVRNAFRPGTVDELYRALDHARQTPDVGCVLLTGNGPSPKDGGWAFCSGGDQRI
                     RGRTGYQYASGDTAETVDPARAGRLHILECQRLIRFMPKVVIAVVPGWAAGGGHSLHV
                     TADLTLASAEHARFKQTDADVGSFDAGYGSAYLARMVGQKFAREIFFLGREYSAADAH
                     RMGMVNAVVPHAELEKVALEWAKEINGKSPTAQRMLKYAFNLQDDGLVGQQLFAGEAT
                     RLAYMTDEAVEGRDAFLEKREPDWSPFPWHY"
     gene            complement(11241..11804)
                     /locus_tag="VFE39_13265"
     CDS             complement(11241..11804)
                     /locus_tag="VFE39_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HET6259614.1"
                     /translation="MRQQAEKRQRSENGAAGNGVAAAAHAEADAYRNGEDRPLGAYVL
                     VMAVFAATVAGAAGLARLRGRGLPDGVGPWDVVLLAAGTHKLSRTISKDSVTAPLRAP
                     FTRYMGRGGPAEVMEEVRATTGLRKSIGELITCPFCLDVWIATGFAIGLVFAPRATRL
                     VAATFAVLTGSDLLQLAYAMAQRTAEG"
     gene            complement(12049..12255)
                     /locus_tag="VFE39_13270"
     CDS             complement(12049..12255)
                     /locus_tag="VFE39_13270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HET6259615.1"
                     /translation="MARYEYRVLQLRESLVGGKLSADKLERLLNDEAQQGWQLKAITS
                     TEVKGRIGPGGVDGLLVTLERPVG"
     gene            12322..13452
                     /locus_tag="VFE39_13275"
     CDS             12322..13452
                     /locus_tag="VFE39_13275"
                     /inference="COORDINATES: protein motif:HMM:NF014404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HET6259616.1"
                     /translation="MQTLPKGANAPLPPGPVAVHVTASAPVDISALLVAETGKVRGDA
                     DFIFYNQPAASGVRYRPDGVDVDPAAVDAEIERVTVTASLDGRGPATFGALGGLRLDV
                     AVAGKPVAVFEPDGLGAETALLCVEIYRRGSQWKVRAVGQGYANGLAGIATDFGISVE
                     DQPAPPPVASPPADAQPAEAGRITLDKGKVSLAKRQTVSLTKGGAPALRRVAMGLGWD
                     PASSGSTIDLDASAILIGSTGTKVDAVWFMNKLALRGLVEHSGDNLSGHGSGDDETIT
                     ADLQALPAAITAIVFVVNSFRGQQFTEVGSAYCRLLDLGSGAELVRFDLSHSEPRTGV
                     VMSVLRRNGPVWEMTAIGEFHDGRTARAMFGVAEDVVRGLAG"
     gene            13484..14185
                     /locus_tag="VFE39_13280"
     CDS             13484..14185
                     /locus_tag="VFE39_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6259617.1"
                     /translation="MKQVRQTDTVNAQVLDALRAAVITGELQPGTLHSVQTLATQLGV
                     SRTPVREALIKLAQQRMVRFERNRGVRVLQTSLHDLEEVFALRLLLEVAATRRASQQI
                     DESGRRELRRLYQAMERCARADDEYRMWEHDRRFHFVVLQASGNLRLAEYVDGLRDMV
                     LRRGVSTAGLSRSLDDIVAEHRAVLDRIEAGDADGAAEAMRAHLLRTAELLIAQEAGT
                     PPGSVDVDLGWTGRT"
     gene            14333..15103
                     /locus_tag="VFE39_13285"
     CDS             14333..15103
                     /locus_tag="VFE39_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HET6259618.1"
                     /translation="MTADLTWLLIKPGRSITDRNSKDAPAPWVDVPTHCVLVETDEGR
                     ILWDTSCPRDWEERWGPTGLQDFFPYDNVSDEEYLDSRLNQMGIGLDQIDYVILSHLH
                     FDHAGNAQMFKNTNAKLVCHEKEKDFAFGFDGLFTGAHLKTDYQGLTFETVSGDTDFL
                     PGVKLIETPGHTVGCMSMQVDLPDTGTMIFTSDAVYMGESYGPPATPAAIVNNLEQFY
                     ASVEKLRAIQEETDATMVFGHDAEQIHQLRTAPEESYT"
     gene            15100..>15263
                     /locus_tag="VFE39_13290"
     CDS             15100..>15263
                     /locus_tag="VFE39_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HET6259619.1"
                     /translation="MSHPDVELTEETIFTWGAPPLKFGAGASDEVGFEMAGYGVKRAL
                     IVTDPGIHALG"
BASE COUNT         2135 a         5537 c         5353 g         2238 t
ORIGIN      
        1 ccgccgccgg cagtgctccc tcgcgtcgct cacctcgctc gtgttcaccg gcacggagct
       61 gactcagctg cgctacagcg agcccgcggg cgcgagcgag ccggggcaga ccggggcatg
      121 aggcggatcg ctatcgctat cggcgtcgcc gcggtgctgc tgctcgcggg gtgctccacc
      181 agcaaggatg cagtggccca gaccgggaat ttcgagttcg tcgctcccgg cgggcagacc
      241 acgatcacct acgacccacc ggccagccgc gggcgggtga acggcatcac cggggagagt
      301 ctgctgcacg acggccagac ggtcgggctg gagaactacc ccggaaaggt cgtggtgctc
      361 aacatctggg gctcgtggtg cggtccgtgc cggacggagg ctccggagct gcagtccgtg
      421 caggaccaga ccggagcggt cgtcctcggg atcgacgtcc gcgacgaccg ccctgccgcc
      481 accgacttcg tccgcgaccg cagcatcacc ttcgactcga tcttcgatgc ccccggccgc
      541 actctggcgg ccctgcgcgg ctacccgcgc aacacggtgc cgtccacgct cgtgctcgac
      601 cggcagcacc gggtggccgc cgtgtacctg accacgatcc gggtgtccga gctggtcccg
      661 ctgatacagc gcctgcaggc cgagcccgcc tgaccccgcg ctttgcggtt acggccgtcc
      721 ggtccggaac gtgccgattc actacacgtt gtagaacccc gtgcacagga agccgggcac
      781 cgggcctacc ctcgtcggtg atggacccca cgaccaccct cgcagtctcc ggcccgctgc
      841 tgctcgcggt cgccgtcgct gtgctggcgg gtgccgtgag cttcgcgtcg ccgtgcgtcg
      901 tcccgctcgt cccgggttat ctcgcgtacc tcgccgggct ggtcggcgcc gacgccccgc
      961 ctgcgacgct cgcccaggca cgggagggcc gcaccgggcg gtggcgcgtc gccggggcgg
     1021 ctgtgctgtt cgtcgccggg ttcacggttg tcttcggtgc gatcctggtg ggcgtcgtct
     1081 ggctgtccga cgcgctggtg gccaacgagg ccgccctgca gcgcatcggc ggggtcgtca
     1141 tgatcgcgat ggggctggcg ttcgtcgggc tcattccggt gctgcagaac gagcgccggg
     1201 tgcactgggt gccgcgcgcc ggggtgtggg gcgcgccgct cctcggggcg gtgttcggcc
     1261 tggggtgggt gccctgcatc ggcccgacgc tcgcgggtgt cgtcgcggtg gctgcgggca
     1321 ctggtggggg ctcgcttcgc ggcgtcgtcc tcacggtggc ctactgcgca gggctcgggt
     1381 tgccgttcgt gctgatcgcg ctgggtgcgt cgcgggccgt ccgcgccctc gggtggttgc
     1441 gccgccacac gcgcgcgatc cagatcgccg gcggggtgct catggtgggt gtcgggttcc
     1501 tcctcgtcag cgggctgtgg ggcgggctgc tggcccagct gcagacgtcc gtcgcaggct
     1561 tcaccccggt gctgtagcga tggcgtcgac ggagatagcg actcgcccgc cggaaacggg
     1621 cgactcgcgg gcgcagaacg ggttactcgc gctgttgcgg aacgcgtggc ggtccctcac
     1681 ctccatgcgg acggcgctca tcctgctgtt cctgctggcg gtggcggcgc tgccgggcgc
     1741 gctgctgccg cagcgctcgc tcaaccagcg cctcgtcgac cagtacttca ccgactaccc
     1801 ggccatcgcg ccggtgctcg accggctggg cttcttcgac gtcttcgcgg ccccttggtt
     1861 cgcggcgatc tacctgctgc tgatggtgtc gctcgtcgga tgcgtgctgc cgcggacgtt
     1921 cgagcacgcg aagaacctgc gcgccgcgcc cgtcgtgacc ccgcgcaact tcgcccggct
     1981 gccgcaccac gccaccgcca ccctcgacgt cggtccggac gaggcggccg agcggatccg
     2041 cggccggctg cgccgctggc gcaggctcga gacggaggcc ggttccagcc gcacgatctc
     2101 cgccgagaag ggctacctgc gcgaagccgg caacctgctg ttccacctca gcctcatcgg
     2161 gctgctcgtc gggttcgggg caggcaagct gtacggctac ggcgggcagg tcatcgtgct
     2221 tgccgacggc ggccagttct gcaacaccgg aatcctcggc tacgactcct tccaggccgg
     2281 tctgcgcgtg gacggcaccg acctcacgcc gttctgcctc cgcgtggacg acttcgaggc
     2341 cacctacctg cccgacggcc aaccggagag ctaccgcgcc gccatcggct atcagacgac
     2401 ggatgacctc aacgccggcc gtgtggacca gtggcgcccg tacccgctgg ccgtcaacag
     2461 cccgctgcgt ctcgacggca gccgcgtcta cctgatcaac cacggctatg cgccgcgctt
     2521 caccgtgacg ttccccaacg ggcagaagcg caccggcgag gtccaatggc agcccgtcga
     2581 cgcgacgacg tacctttcgc agggcgccac caagttcgag ccgcccggca ttacggacga
     2641 gcagaagcgc cgcaccagcc agttggccat cacggggctg ctggcgccca cgagctccgg
     2701 gggcaaggtg gtcacctcgg tgtaccccgc gttgcgcgac cccgaggtgg cggtcgacgt
     2761 gctccgcggt gacctcgggc tcgacgacgg gcgcggccag tcgatcttcc aggtcgatca
     2821 gagcaagatc gattcaggcc agctggtgcg ggtggcgcgc gcgaacctcg tccccggtca
     2881 gtccatcacc cttgacgacg gaacgattat cacgttcgac gacgtgcgac agtggatcgc
     2941 gctgcagatc agccacgacc cggggcagga tgtcgtgctc gtgttcgccg tgctcatgct
     3001 cgtcgggctg gggttgtcgc tgagcatccg gaggcgccgc ttctgggcac ggctcacccc
     3061 caccgaggac ggcacccgta ccgtcgtgga gatcggcggt ctggcccgca ccgacagggc
     3121 gggctatggc gaggagttcg accgcctccg ggcgcacctg ctgaacattt ccgacaccga
     3181 cggctccgat gtcgacggcc ccatcacggc agcgaaggac ggctgatgct caccgaactc
     3241 gccctgtaca gcgaccgcct cttcatggga gccgtcggcg tcttcgtatt cgcgatggtc
     3301 ctgcacggcg ccgagtacgc cgtgcatcgg tcggcgaagg ccgtgctcgt cggagccggg
     3361 ggcccggccc tcgacgagca cgagtcccac ccggagggga cgcccgtccg cagccggtcc
     3421 gagcgcctgg gccgcatggg ggtggcgctg gtcgtgctcg gggccgtgct gctggcggcg
     3481 tcgatcgtca cccgtggtct cgccgcgcag cgctggccgc tcggcaacat gtacgagttc
     3541 acctcggcca tctgcctggc ggccgtggtc gcatggctcg tggtgctgcg ccggtcgccc
     3601 gcgtcgcgac cggtcgggct gttcgtgctg ctgccggtga tcatcctgct gttcctcggg
     3661 ggcacggtgc tctacgcacc ggccgcaccg gtgatgcctg cgctgcagtc gtactggctc
     3721 gtggtgcacg tcacggctgt caccatcgcg tcgggtctcc tgatggtccc cggcgtggcg
     3781 agcctgctgt tcctgctgcg ccgctccggg cggctgaccg gcgtggccga ccgactgccg
     3841 agcgccgacg tactcgaccg gctcgcctac cgcctcacca tcatcgcgtt cccgctctac
     3901 acgttcgcga tcatcgccgg cgcgatctgg gccgaggccg catggggccg attctggggc
     3961 tgggacccga aggagaccgt cgcgttcgtg gcctgggtga tctacgccgc ctacctgcac
     4021 gcccgcgcca cggcaggctg gcgcagcggg ccggccgcgt gggtcaacgt cgccggcttc
     4081 gccgccgtga tcttcaatct gttcttcatc aacatggtgg tcgcgggcct gcactcctac
     4141 gccgggctcg gctgaccgag cgtgtgacac cgggcccacc catccgcgtc tcgaccccga
     4201 gcagtcgcgc gactaccgtc ggcctccgtg cacgtgatca gtggtggacg cgttcggttc
     4261 gacgggatgt cgtgatgacg gagcgtgatt tcgcggatga tcgcgactac ccggacggct
     4321 cagtcggccg ctatgtgcgt gagcggtggg gagccatccc ggcacgcgaa ccccagcctg
     4381 gcgccacccc gcctgaacct cgctcggcgg agccggaggt cgaggtcgag cccgcgctgc
     4441 ccggccccgc cccggacgtg agcagcatcg catcccccgg cacgccggcg gtcccgcccg
     4501 cgtggccgag tcaccccgtg caggacggcc ggctgcgtgg cgcaggcgga ggcggccccg
     4561 tagccgggac accaacggat cgtccggagc cctccgggtc gcgcgccgac gttcctccgc
     4621 ctgctgcgta cccgcccgtc accggtccag ctgctgctcc gatgccgcac aggccgggcc
     4681 gagtggccat aggccccgag cgcattggcc cgcgcacccc gccccggaac gcccggtgga
     4741 tcgatcctgc cgccgtggcg gcgcaggagg cgcagcgggc tcgcagcggc agcgagcaag
     4801 tggcgacgga cctgtcctcg gcgcgactgc tgcgcccgac cgcacgcccg ccgcagtcgg
     4861 gctggcgccg cctggtctac gtgctgtccg ggcgtctgat aaaccccggg gaaagcccgg
     4921 ccgacatccg acgccgcgag ctcaccgctc ggatcaacca gccgttgctg ggctgctaca
     4981 agatcgcgat gctcagcctg aagggcgggg tcggcaagac cacgatgacg gcgacgctgg
     5041 gtgcgacgtt cgcatcactc cgcggcgacc gggtcgtggc cgtcgacgcc aacccggacc
     5101 gcgggacgct cagccagaag atcccgctgg agacgagcgc cacggtgcgt catctcctgc
     5161 gcgacgccca gcgggtccgc cgctacaccg acgtacgggc ctacacctca cagggcccgt
     5221 cccggctgga ggtgctggcc agcgagcagg atccggccgt ttccgaggcg ttcagtgagg
     5281 acgactaccg gcgcacggtc acgttgctcg agcactttta caacatcgtg ctcaccgact
     5341 gcggcaccgg cctcatgcac tccgcgatgt acggcgtgct gggggtggcc gaccagctga
     5401 tcatcgtgtc gtccggatcg atcgacggtg cgcgcagcgc gtcggcgacg atggactggc
     5461 tcgacgccca cgggcacggt gacctggtgc gcaactctgt ggcagtgatc aactgcgtgc
     5521 accggtcggg cggcgtcgac ctgaaccgcg tggcggagca cttcgccgcg cgctgccgtt
     5581 cggtggtacg gatcccgttc gacgcacact tggaggaggg cgccgaggtc gacctcgacc
     5641 gcctcgagcc tgcgacgcgg ctcgcactgc tgaagctggc cgctgccgtg gccgacgcct
     5701 tcgatggtgc cgtagtctga cgggccgtca tccggagccc cggcccatcg ggccgggcta
     5761 ccggctcagg cgccgggtgg atcgtcccgt cgcttgacct tctcgtcgag ctgacgcagg
     5821 aagtcgggat catcgtcggg tccgctgacc cgtggcctgc cggaccccgt gttcgggcgt
     5881 cgcggcggct gcgggacctg cgggcctccg gcgctcatcg ccttccacag caccagggcg
     5941 atgatcgcgg cgccgacgaa cgcgatgagg aacaacactc caaccacctc cgtcgtcgag
     6001 gttagcggag cgcagcgacc agggtggtga agagccggtt cgctgtccgc tccatcacgc
     6061 gagggcgcaa cgaccaggca ctcagggccg cgacggaggc agcgttgcct cactcgtgag
     6121 ctcggccagc atgctgcgca cctccgactc acggaagcga cggtggccac caggggtccg
     6181 gatcgagccg atcctgcccg cggacgccca gcgcgtcacg gtcttggggt cgacccggaa
     6241 gagagcggcc acctcgccgg gagtaagcag gcgttcgttg ctgctggcct gtgtcgtgac
     6301 tgtcatgtgg acctccagag ccgagggcgt ttccgggcca tcgtgacacc tgggaccccg
     6361 agtactcgaa cggttgtccg gaagtaaagg gctcttcaag gacgaaccgg acacgaatcg
     6421 ggcaccgcag cgacgccctc gattcggtcg tacgctcgtg ccatgacctc gccacccacg
     6481 ggacagccgg ccacggggca ggggccgggt cttgctgcca cggtagggct ctacgcgctc
     6541 gcccgcctag ctctggtcgc cgtcgtgacg gtcctcatgc ttttggcggg cacgccgttc
     6601 ctcatcgctg tgctggtcgg tctgatcgtg gcgctgccgc tgtccatggt gctgttccgc
     6661 gggctccgca gccgtctcga cagcgcgctg gcggagagtc gaagtcggcg gtccgcggaa
     6721 cgctccgcct tacgggcgcg gctccgcggc gagggggtcg ctgcggactc aggtgaactg
     6781 gctcagcgcc agcccgatcc cggtgagcac tgaccaggcc aggagcagca tgcccgtctg
     6841 cgccagcacc cggatcagcc tccggccgtc cacacccccc agcacctggc gcgccaggag
     6901 ggcggcgagc ggcaggacca gcaacccgag cagcatcggc aggctgcgca gcccaccaag
     6961 cgccgacagc aggatcggca ctgtgacaag cgcggcgtag aggcgacgcg tctcggcgtc
     7021 cccgagcatg acggcgagtg tgcgtttccc caccaccgtg tcggtgggga tgtcacgcag
     7081 gttgttggcc accagcacgg cacacgtgag caatcccaca ccgacggcgg tgacgaccga
     7141 gagtgctccc ggcgtgccgc tctgggtgat caccgtgccg agcaccgcca cggggccgaa
     7201 gaagacgaaa accgcaatct cgccgaggcc cgagtagccg taggggcgcc gcccgccggt
     7261 gtagaacctg gcccccgcga tgcacagggc gccgacagcg atcagccacc actgcccgct
     7321 cagcgacacc agggtgagcc cggccaaccc ggcgatcgag aagcacccga tcgcgaaggt
     7381 gcgcaccgtt gccggttcgg ccaccttcga cccgaccagg cggagtggac cgacgcgttc
     7441 gtcgtcggtg ccacggatgc cgtcggagta gtcgttggcg tagttcacgc cgaccaccag
     7501 cgccacggcg accaccagcg cgagcagggc acgccccggt gcgacgacgc cgtagccgat
     7561 cgccgcgccg gtgccggcca ggaccggcga gacggcggtc gggagggtcc gcgggcgggc
     7621 gccctcgatc cattgcgcga tggttgccac gcgggaacgt tatccggcgg tgcttccggt
     7681 gttcgccgga gcgccgccgg tgccggtggg gcaggacggc gccgcgtcga agccgggcac
     7741 ggccgccgcg gccaggtact ggtcgacgcc gatccgcaac caccggtccg ctccggcacc
     7801 gagtacgcac tgcaccggga ccgcagcgtg gttcgccgcg atcccgacca cctcggcgcc
     7861 ggctaccggc gccgccagca cgggcaggtc gccggcgacc gtggacaggc gctccgagcc
     7921 cgtccacagg tagtccacgg tcacccaggc gttgtcgttg agcccgagcg cgtcccagaa
     7981 cagcccgtcg gcgaggtcga tgcccgcggg gttgaggacc ttacggttga actggtcctt
     8041 gccgctgttg tagtcgttct ggtacgccgc ctgcgcctcc gggaggccct gcggaaggtc
     8101 gtgccactcc tgacgggtgg gtgcggggtt ccagtagtcg tcccgggtgt tccacggccc
     8161 cacgtcccag accggtgcga aggcacagcg tccgttgggc gcgcacacct tgaccgagta
     8221 gtcgctggtg tcacgcggcg acagtcctcg gcgtgacggc agtgcgacga agtggtcgcg
     8281 ttcggtgatg acgtgcccgt tcgccgtcgt gccgccgacg agcccctcgc gcgtcgcgaa
     8341 aacccggtag ctgagcgcgg cctgctccgg cccgccctcg gtgagctgct tcgtcgtggg
     8401 ctgagcggtg agcgtgagcc catgcacggc ggggccgccg ccgtgcgggc cggtgaggac
     8461 cagcctgccc tgtacctcgg acgtcggctc gggtagctcg gcgaccatgc tggccgtccc
     8521 gggggcacca tcggcggggc cggcagggat ccattccgtc cagccgccgg tgctgcggcg
     8581 cccgcggatg tcgacggcgg ccgcactgcc cgccgggacg tcgccgagga cagttgcgac
     8641 gatccggtcg gtcgtgatgt cgagctgttg cgggaggagc gtgagcagcc cggtcggcac
     8701 gagcgccgca ccgatcggtc ccccctcccc gagtcgggcg aggtaggcgc ccgcggccgc
     8761 cagccgcgcc gtgccgtcgg cgagcgtgac acccgctgcc tcgccggcac cgaggtcggg
     8821 gctccaggac gcgagggatg cggcgtccgc ggattccgga gtcgccacag cgaccgagag
     8881 cgcgccgacc agggccagcg atgccgttgc cgtgcccagc ctcatgctgc gtctccccat
     8941 gttaccgctg tgaccagagt gccttctggg gcgcagcaga tcggatgagc gagtagcagt
     9001 caagcaaccc agagtgacct gcacgatcga gggaattcgg ttgtcactcc tgcgggtggt
     9061 gctgtcgggc gtcgagcagg tccgcgattg cacgccgatc aggcttcccg atgccgcgaa
     9121 gcgggatctc gggcacggtg tgcagcacgc gcggcaccgc ggcccggccg agctcggcca
     9181 gcacggcggc acgcagccgt ccgcctcgga cggcatccgg ctcctgcggt ccgtccccgg
     9241 gatccgccac gacggccgct gccacgacct ggccccactc cacgtccggc agccccacga
     9301 cgcaggccgc ccgtacgccg gcctgggccg ccaggacctg ttccaccgct gccggggcca
     9361 ccttctcgcc gccggtgatg atcatgtcgt cggcgcggcc gagcacctcg agtcgtccgc
     9421 cgcgccaccg gcccaggtcc cccgtgcgga accagccgtc gccgaacgcc gctgcggtgc
     9481 cggccgggtc tccgagatag ccctccgcaa gggtcggtcc acccagccgg atcctgccgg
     9541 tctcgtcgat gtccacccgc acgccgtgca gcggcacacc gtcgtacacg cagccacccg
     9601 ccgtctcgct catgccgtag gtcgtgacga cacgcacgcc cgccgccagc gcgcgggcgt
     9661 gcagccacgg gtccaacgcg gcccctccga cgagcacggc ggcatagccg cgcagggcgt
     9721 cgagtgcggt gccgccggcg tccaggagcc tggctagctg agtggggacg aggctcgtgc
     9781 agcgcctccc gtcgcccagc tgcgcagtgg cggtcgcgaa gtcgtcgggc cggaagccgt
     9841 cccgcagatc ctgcaccatc ggcgccgccc cggcgagcag cgagcgcacg accacctgcg
     9901 cgcccgcgac gtgctcggcg ggaagggcga gcagccagcg tgccggcccc tcgagccgct
     9961 ccacggtggc cagcgcggac gcccgcagcg ccgtggcgga gagcaccacg tacttgggga
    10021 cgccggtgga gcccgaggtg gcgatcacca gcgccgcacc cggtccggcc gccgccgtgg
    10081 gggccgagcc gcggggcggg aacggaagca cggcgggccc gccgtcgagc gcctcacgca
    10141 aagctcccgc gagctcggcg accgcggggg gcgatccgtc cacctgaacc atccggacgc
    10201 cggtcatcgg ggcacccgcc cccgggcccg aaccggtgcg gggcgccgga cgtttatcag
    10261 tagtgccacg ggaacgggga ccagtccggc tcacgcttct ccaggaacgc gtcccgtccc
    10321 tccaccgcct cgtcggtcat gtacgcgagt cgcgtggcct cgcccgcgaa cagctgctgc
    10381 ccgaccagcc cgtcgtcctg caggttgaac gcgtacttca gcatccgctg cgcggtcggg
    10441 gacttgccgt tgatctcctt ggcccactcc agcgccacct tctccagctc cgcgtgtggc
    10501 accaccgcgt tcaccatgcc catccggtgc gcatcggccg cggagtactc gcgaccgagg
    10561 aagaagatct cgcgggcgaa cttctggccc accatccgtg caaggtaggc cgagccgtac
    10621 ccggcgtcga acgagcccac gtcggcgtcg gtctgcttga accgcgcgtg ttccgcactg
    10681 gccagcgtca ggtcggccgt cacgtgcagc gagtgccccc cgcccgccgc ccagccgggc
    10741 accaccgcga tgacgacctt gggcatgaac cggatcagcc gctggcactc caggatgtgc
    10801 agccgccccg cgcgggccgg gtccacggtc tccgcggtgt cgcccgaggc gtactggtag
    10861 ccggtgcgcc cgcgaatgcg ctggtcgccg cccgagcaga acgcccagcc gccgtccttc
    10921 ggggacggcc cgttgccggt gagcagcacg cacccgacgt cgggcgtctg gcgggcgtgg
    10981 tcgagcgcgc ggtagagctc gtcgaccgtg ccgggccgga aggcgttgcg cacctccggc
    11041 cgatcgaacg cgatccgcac ggtgccgcct gccgggccgg cggcggtggc ccggtggtag
    11101 gtgatgtcgg tgaagtcgaa tccatcgacc acccgccacg catcggggtc gaacagctcg
    11161 gacggggcag cggtctccac gacaagcgac cctatgtccg gggtctgcac cctcatggga
    11221 ccgggctcgg gtttgcccgc ctaaccctcg gcggtccgct gcgccatggc ataggcgagc
    11281 tgcagcaggt cggagccggt cagcaccgcg aacgtggcgg cgaccagccg ggtggcccgc
    11341 ggggcgaaga cgagcccgat cgcgaagccg gtggcgatcc agacgtcgag gcagaacggg
    11401 caggtgatca gctcgccgat cgacttccgc agcccggtcg tcgctcgcac ctcctccatc
    11461 acctccgccg gtccgccgcg gcccatgtac cgggtgaagg gtgcgcgcag tggggcggtc
    11521 accgagtcct tgctgatcgt gcgcgacaac ttgtgcgtgc ccgccgcgag cagcaccacg
    11581 tcccatggac cgacgccgtc cggcagccct ctaccgcgaa gccgcgcgag tcccgcggcg
    11641 cccgccaccg tcgccgcgaa gacggccatc acgagcacgt aggcgcccag cggccggtcc
    11701 tccccgttgc ggtaggcgtc ggcctcggcg tgtgcagcgg ccgccacccc gttgccggca
    11761 gcgccgttct cgctgcgctg ccgtttctcg gcctgctgtc gcatcttgac gtcctctcag
    11821 gtctcgcggc gggtgccgta caccggccta cctaccccgc cggaagcccg gtcacgcgcc
    11881 ggaaccgatc gaactcctca ttcgctcgcg agtggtcccg gccgtcctcc tgggccgcgc
    11941 cagcaggcgt tcggccgcgc tgccggacca ctcgacgagc atcagcgtcg catcgtcgcg
    12001 cggctcgacc ctgcggtgct cccgcacggc gtgcgcgggt ggagctgctc agcccaccgg
    12061 acgctccagc gtcaccagca gcccgtcgac cccaccgggg ccgatgcggc ccttcacctc
    12121 ggtggaggtg atcgccttga gctgccagcc ctgctgtgcc tcgtcgttga gcagcctctc
    12181 cagcttgtcg gccgagagct tcccgccgac gaggctctcg cgcagctgca ggacccggta
    12241 ctcgtaacgc gccatgcgtc tcaggctgcc aggccgcccg gcgggtcccg cgccagcatc
    12301 gccgctggca ggatcgctgg cgtgcagacc ctccccaagg gcgcgaacgc gcctctcccg
    12361 cctggcccgg tcgcggtgca cgtcacggcg agcgcgcccg tcgacatctc ggcgctgctc
    12421 gttgcggaga ccgggaaggt ccgcggcgac gccgacttca tcttctacaa ccagcccgca
    12481 gcctccggcg tccgctaccg ccccgacggc gtggatgtcg acccggccgc cgtggacgcg
    12541 gagatcgagc gcgtcaccgt caccgcgagc ctcgacggac gcggcccggc gaccttcggc
    12601 gcactcggcg ggctccgcct cgacgtggcc gtggccggga agccggtcgc cgtgttcgaa
    12661 cccgatgggc tcggtgccga gacggcactg ctctgtgtgg agatctaccg ccgcggcagc
    12721 cagtggaagg tgcgggcggt cgggcagggc tacgcgaacg ggctggccgg catcgccacg
    12781 gacttcggca tctcggtgga ggatcagccc gcgccgccgc ctgtggcgtc cccgcccgcc
    12841 gacgcccaac ccgccgaggc cggcaggatc acgctcgaca agggcaaggt gtcgctggcc
    12901 aaacgccaga cggtctccct caccaagggc ggggcgcccg cactgcgccg ggtggccatg
    12961 ggtctggggt gggaccctgc ctcgagcgga agcacgatcg acctcgatgc gtcggccatc
    13021 ctgatcggga gcaccggcac gaaggtcgac gcggtgtggt tcatgaacaa gctcgccctc
    13081 cgcgggctcg tggagcattc cggggacaac ctgagcgggc acggctccgg ggacgacgag
    13141 acgatcaccg cggatctgca ggcgctgccc gccgcgatca cggccatcgt gttcgtcgtg
    13201 aactcgttcc gcggccagca gttcaccgag gtgggaagcg cgtactgccg gctgctcgat
    13261 ctcggctccg gagccgagct cgtccgattc gacctcagcc actccgagcc gcgcacgggc
    13321 gtggtgatga gcgtgctgcg caggaacggc cccgtgtggg agatgaccgc gatcggcgag
    13381 ttccacgacg gcaggacggc gcgcgcgatg ttcggggtgg ccgaggacgt cgtgcgcggc
    13441 ctggccggat gacgcaggct caccggaagc cccggccgtc gccgtgaagc aggttcggca
    13501 gaccgacacc gtcaacgccc aggtgctcga cgcgctgcgt gccgccgtca tcaccggcga
    13561 gctgcagccc ggcacgctgc actcggtgca gacgctggcc acgcagctcg gcgtctcgcg
    13621 cacgcccgtc cgcgaagcgc tgatcaagct cgcgcagcaa cgcatggtcc gcttcgagcg
    13681 caaccgcggg gtgcgggtac tgcagacctc gctgcacgac ctcgaggagg tcttcgcgct
    13741 gcggctgctg ctggaggtgg ctgccacccg acgcgccagc cagcagatcg atgagagcgg
    13801 gcgccgcgag ctgcggcggc tctaccaggc gatggaacgc tgcgcccggg ccgacgacga
    13861 gtaccgcatg tgggagcacg accgccggtt ccacttcgtg gtgctgcagg cttcggggaa
    13921 cctgcggctc gccgagtacg tcgacgggct gcgggacatg gtgctgcgcc gcggcgtctc
    13981 gacggcgggc ctgtcgcgga gcctcgacga catcgtggcc gagcaccgcg cggtgctcga
    14041 ccgcatcgag gccggtgatg cggacggagc cgccgaggcg atgcgggcgc acctcctacg
    14101 cactgccgag ctgctcatcg cacaggaggc gggaacccca cccgggagtg tggacgtgga
    14161 tctcggctgg accggccgaa cctgatccat cgacgagtgg gcacacattg acgagtggca
    14221 tgccacatgc cacggtcggc ctgggccgcg cgggcgagcg cggcccggtc tcgacgtcga
    14281 gaggaccacg atggcgactg cgaacaaggt gagcgtgatc ccgtgcgggg ccatgaccgc
    14341 ggacctgacc tggttgctga tcaagccggg ccgctccatc accgatcgga actccaagga
    14401 cgcgcccgcc ccgtgggtcg atgtgcccac ccactgcgtg ctcgtcgaga ccgacgaggg
    14461 ccggatcctc tgggacacca gctgcccgcg cgactgggag gagcgctggg ggccgaccgg
    14521 cctgcaggac ttcttcccgt acgacaacgt cagcgacgag gagtacctcg actcccggct
    14581 gaaccagatg ggcatcgggc tcgaccagat cgactacgtg atcctgtcgc acctccactt
    14641 cgaccacgcc ggcaacgcgc agatgttcaa gaacacgaac gcgaagctcg tctgccacga
    14701 gaaggagaag gacttcgcct tcggattcga cgggctgttc acgggcgcgc acctcaagac
    14761 cgactaccag gggctgacgt tcgagaccgt ctccggcgac acggacttcc tcccgggcgt
    14821 gaagctcatc gagacgccgg gtcacacggt cggctgcatg tccatgcagg tggacctgcc
    14881 cgacaccggc accatgatct tcacctcgga cgcggtgtac atgggcgaga gctacgggcc
    14941 gcccgccaca cccgcggcta tcgtcaacaa cctggagcag ttctacgcgt cggtcgagaa
    15001 gctgcgcgcg atccaggaag agacggacgc gacgatggtg ttcggccatg atgccgagca
    15061 gatccaccag ctgcgtaccg cgccggagga gtcgtacaca tgagtcaccc cgacgtggag
    15121 ctcaccgagg agacgatctt cacctggggt gcgccgccgc tgaagttcgg tgcgggcgcc
    15181 tccgacgagg tcggcttcga gatggccggc tacggcgtca agcgggccct gatcgtcacc
    15241 gaccccggca tccacgcgct ggg
//
LOCUS       DASRSC010000271         5705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4038584,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000271 DASRSC010000000
VERSION     DASRSC010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5705
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4038584"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="VFE39_13295"
     CDS             <1..507
                     /locus_tag="VFE39_13295"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6259620.1"
                     /translation="VPDVDAAVAVVRAAGGTASDPTDEPHGRVADCADDLGLPFALWT
                     GPPGPAHTAQVDEAIAHLVLRVPHAGRARAFYSTVLGWGFAPARVLDGWKVRTAGGEP
                     RPRTSIWGGRREPAAVVPTYAVPDLAAAVLAVRAAGGTSTEPERRRFGTAAECTDDQG
                     GRFQLVQR"
     gene            complement(668..1228)
                     /locus_tag="VFE39_13300"
                     /pseudo
     CDS             complement(668..1228)
                     /locus_tag="VFE39_13300"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            1330..2250
                     /locus_tag="VFE39_13305"
     CDS             1330..2250
                     /locus_tag="VFE39_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6259621.1"
                     /translation="MDRIELGTALRTWRERLHPSDVGLPAGLRRRTPGLRREEVAALA
                     GVSVDYLTRLEQGRGPRPSEGVLGALARALRVTDAERDHLFHLAGGAPPLPGRIRSAV
                     RPSVLRLMDRFTDLPALLLDAKYDVLAWNPMAAALLGDLSSIPLDQRNIARQAFTGVH
                     RLDVEPGERERLDRALVSDLRRSAARYPTDPGLQRLVADLRSASADFERLWTLRELDE
                     RHNDTKRIRHPELGVLELDCNVLEVHGDDQVLLVYSAAPGTRAAEALELLRVVGLQHV
                     GRTRTGYPPTGGRGDADFPASGHDTAQGVR"
     gene            2272..2616
                     /locus_tag="VFE39_13310"
     CDS             2272..2616
                     /locus_tag="VFE39_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6259622.1"
                     /translation="MAADEAFDALADPVRREILTLLAQNDECSAGELSDRINRVGRTA
                     VSSHLRVLRTAGLIAERRNGRFRYYSVDATGPAQDVIQLLQELFQGSLDAAKAAVETG
                     AADADGSIRRAM"
     gene            2616..4046
                     /locus_tag="VFE39_13315"
     CDS             2616..4046
                     /locus_tag="VFE39_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6259623.1"
                     /translation="MSRLALTAEQHVDAPPAAVFALFGAGTGAGWLFDAVCDRVALGA
                     VVTLQAPMFGMAAGPVEILGRISAVRSPSRIEIRHDQPWRGRLRVHVDPDGSGTRVRL
                     IADLDDAGLHWLMRRRGFSVPDGGVEGTHPVGLLTSKSGSASVFASATENLAAMAVEE
                     INAEGGIRGRPVRLIVGDDATDPRLGTIEAWRLVHAGCRAIMASTTSATFTRAAHELR
                     GADVLMVQPVMNEGGMGGGLRVQLGERPESQLRAAAAPIMEVAGGRRWFLAGNDYVWP
                     QVVHSAARRVLADHGGTVVGEGFAPLGTRDFAPLIENVLRSGADIVLSSFVGADLVHF
                     ERQCHAMGVRERCRSLALALDEPTRERIGDDAASGMWGVSGYFEQLPGDINEAFLRRY
                     RAAYGAFAPPVSSISESVYEALHLYAIAARRATDDDAVSTARELHTSHMAFPRGMVSV
                     VGPETMQQRLFLAEATPGGFLVSRAG"
     gene            4151..5698
                     /locus_tag="VFE39_13320"
     CDS             4151..5698
                     /locus_tag="VFE39_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HET6259624.1"
                     /translation="MPVQPPELPAIMKVAEGYGLGLSDADVASFAPMVHGVLASWDAV
                     EELHARTAPTAPDRAWARPEDADNPLNAWYVTCNVTEGGEGPLAGRTVAVKDNTAVAG
                     VPMMNGSATLQGFVPARDATIVSRMLAAGATVTGKAVCEDLCFSGGSHTARTGPVRNP
                     WDPSRTTGGSSSGSAALVASGAVDIATGGDQGGSIRMPASFSGIVGHKPTHGLVPYTG
                     AFPIEQTIDHVGPMTRTVADAALVLNVLAGPDGHDPRQPNDLVPDDYVGALARSAEGL
                     RVGVVSEGFGHPGSEPDVDDAVRAAVDTLREAGLTAEDVSIPWHLHGLKIWDVISIEG
                     AAAQMVDANGYGMNWPGLYDPELIEFYGNKWRADGSQFSETVKLVLLSGRYAINRYQG
                     KHYAMARNLVPQLRAAYDEALARYDVLVMPTTPIQASVIPAEDAPREEVIGRALEMVT
                     NCAPLDVSGHPACSVPAGLANGLPTGMMIIGKRFDDATVLRVAHTYEQAVGGFPAPHA
                     ASVGSAG"
BASE COUNT          788 a         2076 c         2034 g          807 t
ORIGIN      
        1 gtgcccgacg tggacgccgc cgtcgcggtg gtacgcgctg cgggtggcac cgcgagcgat
       61 ccgaccgacg agccgcacgg gcgtgtcgcg gactgcgcgg acgacctggg ccttccgttc
      121 gcgctctgga ccgggccgcc cggacccgcg cacacggccc aggtggacga ggcgatcgca
      181 cacctggtcc tgcgagtgcc ccatgcgggc cgggccaggg ccttctacag cacggtcctc
      241 ggttggggat tcgcgcccgc ccgcgtcctc gacgggtgga aggtccgcac cgcggggggc
      301 gagccacggc cccgcacctc gatctggggc ggtcggcgcg agcccgccgc ggtggtgccg
      361 acctacgccg ttcccgacct cgccgctgcc gtgctggccg tccgggccgc gggcggcacc
      421 agcaccgagc ccgagcggcg ccggttcggg acggcggccg agtgcaccga cgaccagggt
      481 ggacggttcc agctcgtcca acgctgacgc cccgcccgct accagggcac cgggccggag
      541 cggtcgaaga accgcccgtc gggccgtcgg gcgccacggt agcgagcgcc acgatggcgt
      601 cgcttccctc ctccaccgtc tggaagccgc tgttgccgtt gaggtccgtg gccgtgtaac
      661 cggggtcggc ggcgttgatc cggaactcgg gcagcgccct ggcgtactgg ctcgtgatca
      721 tgttcagcgc cgtcttggac gacgggtagg tgagtgcgac gaacgtcgac tccacccggg
      781 ccgggtcacc agtgagcgtg atcgagccca tcccgctcga caccatcacg atccgcggcg
      841 cggccgaccg ccgcagcagc ggcacgaacg cccgggtcat ccggaccgga ccgaacacgt
      901 tcgtcgcgta gcaggcgagc atctcgtctg ctccggtgtc caccgccgcg accgccggcc
      961 cgatgatccc ggcgttgttc accagcacgt ccagccgtcc gtagcgggac tccacctcct
     1021 tcgccgcggc cgcgacagac tcgtcgctgg tcacgtccag cgggagcacg gtcacgtcgc
     1081 cgtgcagcgt ctcggcagcg gcggtgccgc gctcggcgtc gcgcgcaccc aggagcactg
     1141 tccagccgcg ctcggccagc cgccgcaccg tcgcgaaccc aatgcctttg ttcgccccgg
     1201 tcacgagggc gatcgtcgtc tccgtcatgc ctccacgatc gggtccgacc ggcgcccgga
     1261 ccaggtccga cccagcctgg gacggcttgt accaggctgg gctgcagtgg gggcggcatc
     1321 ctggatgccg tggaccgcat cgagctcggc accgcgctgc gcacctggcg cgagcgcctg
     1381 cacccgtccg acgtaggtct gcccgccggg ctgcggcgcc gcaccccggg tctgcggcgc
     1441 gaggaggtgg cagcgctcgc gggggtatcc gtcgactacc tgacgcgact cgagcagggc
     1501 cgcggcccac gcccgtcgga gggggtgctg ggcgccctgg cccgcgcgct gcgcgtcacc
     1561 gacgccgagc gcgaccacct cttccacctg gccggcggcg cgcctccgct gccgggacgc
     1621 atccgcagcg ccgtgcgacc gagcgtgctg cggctgatgg accgcttcac cgacctcccc
     1681 gccctcctgc tcgacgcgaa gtacgacgtg ctggcctgga accccatggc ggccgcgctg
     1741 ctcggcgacc tgtcgtcgat cccgctcgat cagcgcaaca tcgcgcggca ggccttcacc
     1801 ggtgtccacc gtctcgatgt cgagcccggc gagcgcgagc ggctcgaccg cgcgctggtc
     1861 tccgacctgc gccgctccgc ggcgcgctat cccaccgacc ctggcctgca gcggctcgtg
     1921 gcggatctcc ggtcggcgag cgccgatttc gagcgcctct ggacgctgcg cgagctcgac
     1981 gagcggcaca acgacacgaa gcggatccgc cacccggagc tcggcgtact ggagctggac
     2041 tgcaacgtgc tcgaggtgca cggtgacgat caggtgctgc tggtctactc cgccgcgccc
     2101 ggcacgcggg ccgcggaggc cctcgagctg ctcagagtgg tcggactgca gcacgtcgga
     2161 cggacccgga cgggctaccc tccgacaggg ggccgagggg acgctgactt cccggcatcg
     2221 ggtcacgata ctgctcaggg ggtgcggtag tgcgcaggag ggacgaccga gatggctgct
     2281 gacgaggcgt tcgacgccct cgcggatccg gtccgccgcg agatcctcac gcttctcgcc
     2341 cagaatgacg agtgcagcgc aggcgagctg tccgaccgga tcaaccgggt cggccgcacg
     2401 gccgtgtcca gccacctccg cgtgctgcgc accgcaggcc tcatcgccga acgccgcaac
     2461 ggccgcttcc gctactactc cgtggacgcc accggtcccg cccaggatgt gatccagctg
     2521 ctgcaggagc tgttccaggg gtcgctcgac gctgccaagg ccgccgtgga gaccggcgcg
     2581 gccgatgccg acgggtccat ccgccgagcc atgtgatgag ccggctggct ctgaccgccg
     2641 aacagcacgt cgatgcgccg cctgcggcgg tcttcgcgct gttcggggcg ggaacgggcg
     2701 cgggttggct gttcgacgcg gtgtgtgatc gcgtcgctct cggcgccgtc gtcacgctgc
     2761 aggctcccat gttcggcatg gccgcaggac cggtggagat ccttggccgc atctcggcgg
     2821 tccgttcgcc ctcgcgcatc gagatccggc acgaccagcc gtggcgcggc cggctccggg
     2881 tgcacgtcga cccggacgga agcggcacgc gggtgcgcct gatcgccgat ctcgacgatg
     2941 ccggactgca ctggctgatg cgccgccgcg gcttcagcgt gcccgacggc ggggtggagg
     3001 ggacgcaccc ggtgggcctg ctgacgagca agtccgggtc ggcgagtgtg ttcgcctcgg
     3061 ccaccgagaa cctggccgcg atggccgtcg aggagatcaa cgccgagggc ggcatccggg
     3121 gcaggccggt gcggctgatc gtcggggacg acgccaccga cccgcggctc ggcacgatcg
     3181 aggcgtggcg gctggtgcat gcgggctgcc gggcgatcat ggcgagcacg acatcggcca
     3241 cgttcacccg cgccgcgcac gagctgcgtg gagcagatgt gctgatggtg caacccgtca
     3301 tgaacgaggg cggcatgggc ggtggtctgc gcgtccagct cggcgaacgc ccggagagcc
     3361 agctgcgggc cgccgccgcg ccgatcatgg aggtagcagg ggggcgccgg tggttcctgg
     3421 ccggcaacga ctacgtgtgg ccgcaggtgg tgcactcggc ggcgcgtcgg gtgctggccg
     3481 accacggcgg caccgtggtg ggagaaggct tcgcgccact gggcacccgc gacttcgcgc
     3541 cgctcatcga gaacgtcctg cgctcggggg ccgacatcgt cctgtcctcg ttcgtcggtg
     3601 ccgacctcgt gcatttcgag cggcagtgcc acgcgatggg cgtgcgcgag cgctgccggt
     3661 cactcgccct cgccctcgac gagcccacgc gcgaacggat cggcgacgac gcggcgagcg
     3721 ggatgtgggg ggtctcgggc tacttcgagc agctccccgg tgacatcaac gaggcgttcc
     3781 tgcggcgata ccgcgcggcg tacggggcgt tcgcgccgcc ggtctcgagt atctccgagt
     3841 cggtctacga ggcattgcac ctctatgcca tcgccgcgcg gcgggctaca gacgatgacg
     3901 ccgtgtccac ggcacgcgag ttgcacacca gccacatggc cttcccccgc ggcatggtgt
     3961 ccgtcgtcgg cccggagacg atgcagcaac gcctcttcct cgcggaggcg acgccgggcg
     4021 gcttcctcgt gagtcgcgct ggttgacggc acgtcacaag tgcgtcaacg cgccagcact
     4081 cgttgacggg ttgcttacca cctggttacg gtccgatccg atcggcgccg aacacgagga
     4141 gacccagacg atgcccgttc agccccccga actgccggcc atcatgaagg tggccgaggg
     4201 ttatggcctg ggcctgtccg acgccgacgt ggcgtcgttc gccccgatgg tgcacggcgt
     4261 cctggcgtca tgggacgccg tcgaggagct ccacgcgcgg acggcgccga ccgccccgga
     4321 ccgcgcctgg gcccgtcccg aggatgccga caacccgctc aacgcctggt acgtcacctg
     4381 caacgtcacg gagggcggcg agggcccgct cgccggccgc accgtggcgg tcaaggacaa
     4441 caccgcggtg gccggcgtgc cgatgatgaa cggctcggcg acgcttcagg gcttcgtccc
     4501 ggccagggac gccacgatcg tctcccggat gctcgcggcg ggcgcgacgg tcaccggcaa
     4561 ggcggtctgc gaggacctct gtttctcggg cgggtcgcac accgcgcgca ccggccccgt
     4621 gcgcaacccg tgggacccca gccggaccac gggcgggtcc tccagcggga gtgcggcact
     4681 cgtcgcatct ggcgccgttg acatcgccac cggtggcgac cagggcggtt ccatccgcat
     4741 gcccgcttcc ttcagtggga tcgtcgggca caagccgacg cacggcctcg tgccctacac
     4801 cggtgcgttc ccgatcgagc agacgatcga ccacgtcggg cccatgacgc gcacggtggc
     4861 cgacgccgcg ctggtactca acgtcctcgc cggacctgac gggcacgatc cgcgccagcc
     4921 gaacgatctc gtgcccgacg actacgtggg cgcgctggcc cgctcggcgg aggggctgcg
     4981 cgtcggcgtc gtctccgagg gcttcggcca cccgggctcc gagcccgacg tggacgacgc
     5041 ggtccgcgcc gcggtcgaca cgctgcgcga ggccggcctc accgccgagg acgtctcgat
     5101 cccatggcac ctgcacggcc tgaagatctg ggacgtcatc tccatcgagg gcgccgccgc
     5161 gcagatggtg gacgccaacg gctacggcat gaactggcca ggcctctatg accccgagct
     5221 catcgagttc tacggcaaca aatggcgcgc ggacggcagc cagttctccg agaccgtcaa
     5281 gctggtgctg ctcagcggcc ggtacgcgat caacaggtac cagggcaagc actacgcgat
     5341 ggcccgcaac ctggtcccgc aactgcgggc cgcctacgac gaggcgctcg cgcgctacga
     5401 cgtcctggtc atgccgacca cgccgataca ggcgtcggtc atcccggcgg aggacgcccc
     5461 gcgtgaggag gtgatcggtc gcgcgctgga aatggtgacc aactgtgcac ctctggacgt
     5521 ctccggccac ccggcgtgca gcgtgcccgc ggggctggca aacggcctgc cgaccggtat
     5581 gatgatcatc ggaaagcgtt tcgacgacgc caccgtgctg cgcgtggccc acacgtacga
     5641 gcaggcagtc ggcggattcc ccgccccgca tgccgcgtcc gtgggcagcg cgggctgacg
     5701 aagcc
//
LOCUS       DASRSC010000272         8579 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1744336,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000272 DASRSC010000000
VERSION     DASRSC010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8579
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744336"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFE39_13325"
     CDS             complement(<1..554)
                     /locus_tag="VFE39_13325"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET6259625.1"
                     /translation="MRALKLLGLIVVVALTGLLSGGASGAAHTRAAIGTVAAVMSPAR
                     LSAEHPAQPAPAAPAHAVPAAASGPARAPAAPAPAAAPVAPRAVPGTPCVSTARACIE
                     LSSNRAWLISAGAVQYGPVPITHGRAGHLTPPGTFNVTFKNRNHRSSIFNNAPMPYSV
                     FFNGGIAFHEGSLRVLSHGCIHLSR"
     gene            complement(720..1796)
                     /gene="glpX"
                     /locus_tag="VFE39_13330"
     CDS             complement(720..1796)
                     /gene="glpX"
                     /locus_tag="VFE39_13330"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HET6259626.1"
                     /translation="MSPSENGNVTRERRREAPDRNLAMELVRVTEAAAMAAGRWVGRG
                     DKNGGDGAAVDAMRQLIGSVSMRGVVVIGEGEKDEAPMLFNGEEVGNGEGPWCDVAVD
                     PIDGTTLMAKGMPNAVAVLAVAERGAMFDPSAVFYMEKLAVGPEAADVIDITAPVAEN
                     IRRVAEAKHIDVTDVTVCVLDRPRHDSLVKEIRRTGARIQLISDGDVAGAISAARPNT
                     GVDLLYGIGGTPEGIITAAAMKCMGGAMQGRLWPTSDEERAKALDAGHDLDRVLTTDD
                     LVRGDNVFFCATGITDGDLLRGVHYRAGGCTTQSIVMRSKSGTVRMIDGYHRLTKLRE
                     YSSVDFDLSDEQLAAVEHSAPPLP"
     gene            1952..2602
                     /locus_tag="VFE39_13335"
     CDS             1952..2602
                     /locus_tag="VFE39_13335"
                     /inference="COORDINATES: protein motif:HMM:NF025396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4245 domain-containing protein"
                     /protein_id="HET6259627.1"
                     /translation="MSSEPDPTTRPPTRPAAGPPSKPPRSAMTLRDMLAALGVLVLVV
                     LAVGGVSRACSFAPTGPTVDSSRLPVVDAPAELRRLAPLVPLPVRIPAVPPDWRANSV
                     DQDLVAGGGRAVRTGYVTPEGRYLRLLQSDASEPALLAVEAGREPVPGRGPVDVDGQQ
                     WVVYGGQGTAGARNEPIWIAEVATPNGPSVRMLITGSGTDDDFRTLARAATTPEPT"
     gene            complement(2704..2919)
                     /locus_tag="VFE39_13340"
     CDS             complement(2704..2919)
                     /locus_tag="VFE39_13340"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET6259628.1"
                     /translation="MTEQPGVDGLGYEQARDELAEVVRTLETGGLGLDESVALWERGE
                     ALARRCEEQLAGARERVHKVLDATQPD"
     gene            complement(2916..4274)
                     /gene="xseA"
                     /locus_tag="VFE39_13345"
     CDS             complement(2916..4274)
                     /gene="xseA"
                     /locus_tag="VFE39_13345"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET6259629.1"
                     /translation="MTQPNSPEQPWPVRTVARKIAEWVDRLGAGWVEGQLAQVTARAG
                     TGTAFLVLRDPAADVSLQLTAPIGLVRDSGPAVAEGNRVVVHGKPSFFLGRGTLNLRV
                     DQIRAVGIGELLARIERLRQLLAAEGLFDPARKRRPPFLPGCIGLVTGRASAAEHDVV
                     SNATARWPSVRFRIEHTATQGALAVPQIVDALGVLDRDPQVDVIVLARGGGSVEDLLP
                     FSDETLCRAVAACRTPVVSAIGHEPDTPLVDHVADVRCSTPTEAGRRLVPDLAEETAR
                     IAGLRDRARRALAGWVDREQRLLTALRGRPVLADPLQALDARATEVERLREAARACLL
                     RGLDRRGSDLEHVRARLTTLGPAATLARGYAVVQRVPDVAEPGTVLPVLRSVTEVEPG
                     ARLRIRVADGAVAATVADEGGSQGSGTGNGADIGNGNGAVEGGTAKPAPTRPRKKKAS
                     TT"
     gene            complement(4271..4882)
                     /locus_tag="VFE39_13350"
     CDS             complement(4271..4882)
                     /locus_tag="VFE39_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid droplet-associated protein"
                     /protein_id="HET6259630.1"
                     /translation="MNALPLPVRIAAGLVATAVEQARDLPRLVVEFPVTAVSQALQAS
                     MRVQQKVTELAIKGDRALGTLRPVEERPSWAVFDDDEPVQQRHNGASTVTALRPQVRD
                     TLVTLGDEQPTVDDSVETGARTTSAAATHPAPEQAEPVASTPSGPNVLPGYQDLTIPQ
                     LRGRLRHLSADDLQILLDWETGHDNRPPFVTMLSNRIITVTEG"
     gene            4980..5984
                     /locus_tag="VFE39_13355"
     CDS             4980..5984
                     /locus_tag="VFE39_13355"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HET6259631.1"
                     /translation="MSGADKRVLLAKPRGYCAGVDRAVEAVEQALEQHGPPVYVRKQI
                     VHNVHVVETLSERGAIFVDEADEVPEGALVVFSAHGVSPAVRDQARRRELRTIDATCP
                     LVTKVHQEAKRFAREDYDILLVGHHGHEEVEGTSGEAPAHIQLVETAADVAGVTVRDP
                     EKVVWLSQTTLSVDETMETVRALRERFPSLQNPPSDDICYATQNRQVAIKAMAAQCDL
                     VLVVGSKNSSNSVRLVEVALQAGAGAAYLIDYAREIDESWLDGVGTIGLTSGASVPEV
                     LVRGVLDHLAERGWTSMEEVTTAEETLTFSLPRELRPARSGQRLSLQPLSPQQSHAGR
                     "
     gene            complement(5866..6456)
                     /locus_tag="VFE39_13360"
     CDS             complement(5866..6456)
                     /locus_tag="VFE39_13360"
                     /inference="COORDINATES: protein motif:HMM:NF042451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6542 domain-containing protein"
                     /protein_id="HET6259632.1"
                     /translation="MTERGERRAGGRWPMADRSVLATVLGVPPVAAVGIGAALTALGV
                     FVDLARIGSLGVVFTICYIAGCVLAVAWVRRNGLFGPMVQPPLLVAVAVPVVVLLAGS
                     PKPGAGVTQRLLVIGAPLVNAFPIMAWTTGIVVAIGVVRLIVQRPLDSPSAPGEPVSA
                     RRGSAAGRGAAGKGAARSGRGAARGAVRTSESPPRS"
     gene            complement(6453..7979)
                     /locus_tag="VFE39_13365"
     CDS             complement(6453..7979)
                     /locus_tag="VFE39_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET6259633.1"
                     /translation="MDTATISVLLIGVIVGAVLGAGVTWFLVTSRARLAAAEAGRAAA
                     DASAMARADAAGLRAERAGLLERLDDLTEQHDRAVERLRRAESEAAGAAAALRSEREA
                     GAQREELLTRRDTELKQAFQALSADALARNNEQFVALAEGRIKEATAALAAKADGDAV
                     SRAQAIAQLLDPMASTLQRVEGQLRTVEKERESAYAGLREQVSAMRFSSEQLQHETKA
                     LVNALRAPQVRGRWGELQLERIVQLAGMVEHCDFSTQVTGQGQDGGVRPDMVVHLAGG
                     KQVVVDAKVPFAAYLEAVESRDPDVHRERLAAHARQLRQHVDALSAKNYWAAFEPSPE
                     FVVLFVPGDPFLEAALRSDPALLEHAFSHNVVVATPTTLIALLRTVAYSWRQEALARN
                     AAQVHQLGKELHSRLATMGTHVAKLGRSLDSAVASYNQTVSSLEARVLVTARKFTDLE
                     VADDEIAAPGQVERAPRTVAAPELVASAADSLIALHELSRTERPTGEAHPEPRATVTG
                     "
     gene            complement(8174..>8579)
                     /locus_tag="VFE39_13370"
     CDS             complement(8174..>8579)
                     /locus_tag="VFE39_13370"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET6259634.1"
                     /translation="SLDELLPQIHPTLRPTELVAGDLGPVADAVTRYHSGELSAVDAV
                     PVRQRSGEFLEHAWEVLRTVPAGGPVTYTEYAAKAGRPAAVRAAASACARNAVALFVP
                     CHRVLRTDGTLGGFRWGVDAKRWLLAHESPRQ"
BASE COUNT         1159 a         3077 c         3129 g         1214 t
ORIGIN      
        1 cgcgagaggt ggatgcagcc gtgcgagagc accctgaggc tgccctcgtg gaaggcgatg
       61 ccgccgttga agaagacgga gtacggcatc ggcgcgttgt tgaagatcga gctgcggtgg
      121 ttgcggttct tgaacgtcac gttgaaggtg ccgggtgggg tgaggtggcc cgcgcgcccg
      181 tgggtgatgg gtaccgggcc gtactgcacg gccccagcgc tgatcagcca cgcgcggttg
      241 ctggacagct cgatgcaggc gcgggcggtg gacacgcacg gcgtgccggg gaccgcacgg
      301 ggtgcgaccg gggcggccgc aggagcgggc gcagccggtg cccgggccgg ccccgaagcg
      361 gcagccggca ctgcgtgcgc gggggctgcc ggtgcgggct gtgccggatg ctcggcggac
      421 agtcgggcag gcgacatgac ggctgccacg gtcccgatgg ccgccctggt gtgtgccgcg
      481 cccgatgccc cgccgctgag cagtccggtc agggccacca ccacgatgag ccccagcagt
      541 ttcagtgcac gcacgccacc accccagccc ctgtgatcgg cggctcggcg tagtcggctg
      601 cagcccccgg tgcacgttaa ctgtgcaagt ccattgagga tccgcgcaag atgcggctgg
      661 atttatcctg gaactcccgt gagatgctca cgattcaatc acgcagggca catcttcgct
      721 cacggaagcg gcggtgcgga gtgctcgacg gcggcgagct gctcgtcgga caggtcgaag
      781 tcgaccgagg agtactcgcg gagcttcgtc agccggtggt agccgtcgat catccgcacg
      841 gtgccggact tcgaccgcat cacgattgac tgcgtcgtgc accccccggc ccggtagtgc
      901 acaccgcgca ggaggtcgcc gtcggtgatg ccggtggcgc agaagaacac gttgtcgccg
      961 cgcacgaggt cgtcggtggt gagcacccgg tccaggtcgt ggcccgcgtc gagggccttg
     1021 gcccgctcct cgtcgctggt cggccagagc ctgccctgca tcgcgccgcc catgcacttc
     1081 atggccgcgg cggtgatgat cccctcgggc gtgccgccga tcccgtagag caggtcgacc
     1141 cccgtgttcg gacgggcagc agagatagcc ccggcaacgt cgccgtcgga gatgagctgg
     1201 atgcgcgcgc ccgtgcggcg gatctccttc accagcgagt cgtggcgggg tcggtcgagc
     1261 acgcagaccg tgacgtccgt gacgtcgata tgcttcgcct ccgcgacgcg gcggatgttc
     1321 tccgcaaccg gcgcggtgat gtcgatgacg tccgccgcct ccggacccac ggccagcttc
     1381 tccatgtaga acacggctga cgggtcgaac atcgcgccgc gctcggccac ggcgagcacg
     1441 gccaccgcgt tcggcatgcc cttcgccatc agcgtggtgc cgtcgatcgg gtcgacggcg
     1501 acgtcgcacc acggcccctc gccgttgccc acctcctcgc cgttgaacag catgggcgcc
     1561 tcgtccttct cgccctcgcc gatcacgacg acgccgcgca tcgacacgct gccgatgagc
     1621 tggcgcatgg cgtcgacagc cgcgccatcc ccgccgttct tgtcgccgcg gcccacccag
     1681 cgcccggcgg ccatggccgc ggcctcggtg acccggacga gctccatcgc caggttgcgg
     1741 tcgggcgcct cccggcgccg ctcgcgagtg acgttgccgt tctccgatgg actcatctgc
     1801 ggcctcctct tccccgtgcc gagcactcgt ggtgcccgtg ggagccgttt gatttgcacc
     1861 gatcctggca gatcggcatc accaacggtg ctcgcgcggc cggaaactgc cgtagctgag
     1921 acgatgcgca cgtgctggga cgatggggct cgtgagctcc gagcccgatc cgacgacgcg
     1981 gccgccgacc cgcccggccg ccggtccacc gtccaagccg ccgcggtcgg cgatgacctt
     2041 gcgcgacatg ctcgcggcgt tgggcgtgct ggtgctcgtc gtcctcgcgg tcggaggggt
     2101 gtcgcgggcc tgctcgttcg cgccgaccgg gccgaccgtc gacagtagcc gcctccccgt
     2161 cgtcgacgct ccggccgagc tgcgccggct cgcgccgctc gtcccgctgc ccgtccggat
     2221 tccggcggtg ccgccggatt ggcgggcgaa ctccgtggac caggacctcg tcgccggggg
     2281 cggccgggcc gtccgcaccg gctacgtcac gcccgaaggc cgctacctgc ggctgctgca
     2341 gagcgacgcc tccgagcccg cgctgctggc cgtcgaggcc gggcgggaac ccgtgccggg
     2401 gcgcggcccg gtagacgtcg acgggcagca gtgggtggtc tatggcggtc agggcacggc
     2461 cggagcgcgc aacgagccga tctggatcgc ggaggtcgcg acgccgaacg gcccgtcggt
     2521 ccgaatgctc atcacgggca gcgggacgga tgacgatttc cgcacactgg caagagcagc
     2581 cacaacgccc gagccgacct gacgcccgga cgcgacatgg acacatgtgt ctgaacggat
     2641 cagcctcgga acagcgacag gtgtccatgt cgccagtggg aggggcgagg gagggaagtg
     2701 gcgtcagtcg ggctgggttg cgtccaggac cttgtggacg cgttctctgg caccggcgag
     2761 ttgttcctcg caccggcggg ccagtgcctc gccgcgctcc cacagcgcca ccgactcgtc
     2821 caggccgagc ccgcccgtct ccagggtgcg caccacctcg gcgagctcgt cgcgggcctg
     2881 ttcgtagccc agcccgtcga cgccaggctg ttcggtcatg tcgtgctcgc cttcttcttg
     2941 cgtgggcgcg tgggcgccgg cttcgccgtg ccaccctcga cggccccgtt gccgttgccg
     3001 atgtcggcac cgttcccggt gccgctgcct tgcgagccgc cctcgtccgc gaccgtggcc
     3061 gcgactgccc cgtcggccac ccggatccgc agccgtgctc ccggctcgac ctctgtgacc
     3121 gaccgcagga cgggcagcac cgtccccggc tcggcgacgt cgggcacccg ctgcaccacg
     3181 gcgtatccgc gcgccagcgt cgcggccggg ccgagcgtcg tcaaccgggc tcgaacgtgc
     3241 tccaggtcgc tgccgcgccg gtccagccca cgcagcaggc aggccctggc ggcctctcgc
     3301 agccgctcca cctcggtggc acgggcgtcg agggcctgca gcgggtcggc cagcacgggc
     3361 cgcccgcgca gcgcggtgag cagccgttgc tcgcggtcca cccacccggc cagtgcgcgc
     3421 cgggcacggt cgcgcagccc ggcgatgcgc gcggtctcct cggccaggtc cggcacgagg
     3481 cggcggcctg cttcggtggg cgtggagcag cggacgtcgg cgacgtgatc gaccagcggg
     3541 gtgtccggct cgtgcccgat cgccgacacg acgggagtgc ggcacgctgc gacggcgcgg
     3601 cacagcgtct cgtcggagaa cggcaacagg tcctcgacgc tgccgccacc gcgggccagc
     3661 acgatcacgt cgacctgcgg gtcgcggtcc agtacgccga gcgcgtcgac gatctggggt
     3721 acggccagtg ccccctgtgt ggcggtgtgc tcgatccgga agcggacgga cggccagcgc
     3781 gctgtcgcgt tcgacacgac gtcgtgttcc gccgccgacg cccgccccgt gaccagcccg
     3841 atgcacccgg gcaggaacgg cgggcggcgc ttgcgagccg ggtcgaacag accctcggcc
     3901 gccagcagct ggcgcaaccg ttcgatcctg gccagcagct ccccgatgcc gacggcgcgg
     3961 atctggtcga cgcgcaggtt cagcgtgccg cggccgagga agaacgacgg cttgccgtgg
     4021 acgacgacgc ggttgccctc ggccaccgcc ggaccggagt cgcgaacgag cccgatcggt
     4081 gcggtgagct gcagcgacac gtctgcggcg gggtcgcgca gcacaaggaa cgcggtcccg
     4141 gtgccggccc gggcggtgac ctgcgcgagc tggccctcca cccacccggc cccgagccgg
     4201 tcgacccact cggcgatctt cctggcgacc gtgcggaccg gccacggctg ctcgggggag
     4261 ttcggctgcg tcacccttcg gtgaccgtga tgatccggtt cgagagcatc gtgacgaacg
     4321 gcgggcggtt gtcgtggccc gtctcccagt ccaggaggat ctgcaggtcg tcagcgctga
     4381 ggtgacgcag ccggccgcgc agctggggga tcgtcaggtc ctggtaaccc gggaggacgt
     4441 tggggcccga cggggtcgac gctacgggct cggcctgctc gggtgccggg tgtgtggccg
     4501 cggcggatgt tgtcctcgcg cccgtctcca ccgagtcgtc cacggtcggc tgctcgtcgc
     4561 cgagtgtcac gagggtgtcg cgaacctgcg ggcgcagtgc ggtgacggtg cttgcgccgt
     4621 tgtgccgctg ctgcaccggc tcgtcgtcgt cgaagaccgc ccagctgggc cgctcctcga
     4681 ccgggcgcag cgtgcccagt gcacggtcgc ccttgatggc cagttcggtg accttctgct
     4741 gcacgcgcat ggacgcctgc agggcctggc tcacggcggt gacggggaac tcgacgacga
     4801 gccggggcag gtcccgggcc tgttccacgg cggtggcgac aaggccggcg gcgatccgca
     4861 ccggcagcgg cagcgcgttc atgcccccag tctcccttgc cccgcgcccg cgagcagccc
     4921 gggggtttcg gacaacgcgc aacgaatgcg tagaggttcg gggccgtagg gtgaggggca
     4981 tgtccggagc cgacaagcgc gtcctgctgg ccaagccgcg cggttactgc gcgggtgtcg
     5041 accgagccgt cgaggctgtg gagcaggcgc tcgagcagca cggcccgccg gtgtacgtcc
     5101 ggaagcagat cgtccacaac gtgcacgtcg tggagacgct cagcgagcgc ggcgcgatct
     5161 tcgtggacga ggccgacgag gtgcccgagg gtgcgctcgt ggtgttctcg gcccacgggg
     5221 tgtcccctgc ggtgcgcgac caggctcgtc ggcgcgagct gcgcacgatc gacgccacct
     5281 gcccgctcgt gaccaaggtc caccaggagg ccaagcgttt cgcccgcgag gactacgaca
     5341 tcctgctggt cggccaccac gggcacgagg aggtggaggg cacctcgggc gaggcacctg
     5401 cccacatcca gctggtcgag accgccgccg acgtcgccgg cgtcaccgtg cgcgacccgg
     5461 agaaggtcgt ctggctgtcg cagaccacgc tgtcggtcga cgagaccatg gagacggtgc
     5521 gcgcgctgcg ggagcggttc ccgtcgctgc agaacccgcc gagcgacgac atctgctacg
     5581 ccacgcagaa ccggcaggtc gcgatcaagg caatggccgc gcagtgcgac ctcgtgctcg
     5641 tcgtggggtc gaagaactcg tcgaactcgg tgcggctggt cgaggtggca ctgcaggccg
     5701 gggcaggcgc ggcatacctc atcgactacg cccgagagat cgacgagagc tggctcgacg
     5761 gcgtggggac gatcgggctc accagcggcg cgtcggtgcc ggaggtgttg gtgcggggcg
     5821 tgctcgacca cctcgccgag cgcgggtgga cctccatgga ggaggtcacg accgcggagg
     5881 agactctgac gttctcactg ccccgcgagc tgcgccccgc ccgctccggg cagcgccttt
     5941 ccctgcagcc cctctccccg cagcagagcc acgccgggcg ctgaccggct ctcccggggc
     6001 gctcggggag tcgagagggc gctgaacgat cagccggacc acgccgatgg ccaccacgat
     6061 cccggtggtc cacgccatga tcgggaacgc gttgaccagc ggcgcgccga tcacgagcag
     6121 ccgctgggtc acgccggcgc ccggtttcgg tgatccggcg agcagcacca cgaccggcac
     6181 ggccacggcg accagcagag gtggctgaac catcgggccg aacaacccgt tccggcgcac
     6241 ccacgccacc gcgagcacgc agcctgcgat gtagcagatg gtgaagacga cgccgaggct
     6301 gccgatgcgg gccaggtcga cgaacactcc cagggcggtc agtgcggcgc cgatcccgac
     6361 cgccgccacc ggcgggaccc ccagcaccgt ggccagcacg gaccggtcgg ccatcggcca
     6421 ccggccgccc gccctccgtt caccgcgttc ggtcatccag tcacggtagc ccggggctcg
     6481 gggtgcgcct ccccggtggg tcgctcggtg cggctcagct cgtggagggc gatcaatgaa
     6541 tcggccgcgg acgccacgag ctctggtgcg gccacggtgc gcggtgcacg ctcgacctgg
     6601 cccggggcgg cgatctcgtc gtcagccacc tcgaggtcgg tgaacttgcg tgcggtgacc
     6661 agcacacgtg cctcgagaga cgagacggtc tggttgtagc tggcaacggc ggagtcgagg
     6721 ctgcgaccga gcttggccac atgggtgccc atggtggcca ggcggctgtg cagttccttg
     6781 ccgagctggt gcacctgggc ggcgttgcgg gccagcgcct cctgccgcca gctgtacgcg
     6841 acggtgcgca gcagcgcgat gagcgtggtg ggtgtggcga ccacgacgtt gtgcgagaac
     6901 gcgtgctcca gcagcgccgg atcggaccgc agcgcggcct cgaggaacgg gtcgcccggg
     6961 acgaacagca cgacgaactc cggcgacggc tcgaacgccg cccagtagtt cttggccgac
     7021 agggcgtcga cgtgctggcg cagctgccgg gcatgcgcgg cgagccgctc gcggtgcacg
     7081 tcgggatcgc ggctctccac cgcctccaga taggccgcga acggcacctt cgcatcgacc
     7141 accacctgct tgcccccggc caggtgcacc accatgtcgg gccggacgcc gccgtcctgg
     7201 ccctgcccgg tgacctgggt ggagaagtcg cagtgttcga ccatgccggc cagctgcacg
     7261 atccgctcca gctgcagctc accccaccgg cccctgacct ggggcgcgcg caatgcgttg
     7321 accagcgcct tggtctcgtg ctgcagctgc tcggagctga accgcatggc gctgacctgc
     7381 tcgcgcaagc ccgcgtaggc cgactcgcgc tccttctcga ccgtgcgcag ctggccttcg
     7441 acccgctgca gcgtggacgc catcgggtcg agcagctggg cgatcgcctg ggcgcgactc
     7501 accgcgtcgc cgtcggcctt ggcggccagc gccgcggtgg cttccttgat gcggccctcg
     7561 gcgagcgcca cgaactgctc gttgttgcgg gccagtgcgt cggccgagag ggcctggaac
     7621 gcctgcttga gctcggtgtc gcgccgcgtg agcagctcct cccgctgcgc tcccgcctcg
     7681 cgctcgctgc gcagcgcggc cgcggcgccc gctgcctccg actcggcgcg gcgcagccgt
     7741 tcgacagccc ggtcgtgctg ttcggtgagg tcgtcgaggc gctccagcag ccccgcgcgc
     7801 tcggcgcgca ggcctgccgc gtcggcgcgg gccatcgccg aggcgtcggc cgcggcccgc
     7861 ccggcctccg cggccgcgag ccgggcgcgc gaggtgacga ggaaccaggt gacgcccgcg
     7921 ccgagcaccg cgcccacgat cacgccgatg agcagcacgg agatggtggc ggtgtccacg
     7981 gagatcagcg tgcaccggcc ctccgacgga acctggccag gcgcgccggg cccggggtcc
     8041 agcggacggc gcgaatcggt gaacggtcga acagatggtc gaggtcgacg gtcacccggt
     8101 cgagcgaacg gcacactcgt ccgttcctgc tggacgagcg cgccgttcgt gcgtactcac
     8161 gaccgctccg gcgtcactgc cggggcgact cgtgcgccag cagccagcgt ttcgcgtcga
     8221 cgccccagcg gaagccgccg agcgtgccgt ccgtgcgcag gacgcggtgg cagggcacga
     8281 acagcgcgac ggcgttacgc gcacatgccg acgctgccgc ccgcaccgcg gcgggccggc
     8341 cggccttggc cgcgtactcg gtgtaggtga cgggcccacc tgccggcacc gtccgcagga
     8401 cctcccaggc gtgctccagg aactcaccgg agcgctgccg gacgggcacg gcgtccacgg
     8461 cggacagctc gcccgagtgg taccgggtga ccgcgtcggc gacggggccg aggtcgcctg
     8521 cgacgagctc ggtgggccgc agggtcggat ggatctgcgg caggagctcg tccagcgac
//
LOCUS       DASRSC010000273        22786 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3220614,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000273 DASRSC010000000
VERSION     DASRSC010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 22786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22786
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3220614"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VFE39_13375"
     CDS             complement(<1..373)
                     /locus_tag="VFE39_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259635.1"
                     /translation="MPPEPSVPASTTPVQDYLDRPARGATEDHLVVPRSLAQSMPLRW
                     QQVFVGLLADLHDAYGHLAWPDYRVLPSRWERLVDLDEEQLADAGYVADLGQDGQLEY
                     RDSADNVVADPENRRVLAPVAD"
     gene            complement(411..1424)
                     /locus_tag="VFE39_13380"
     CDS             complement(411..1424)
                     /locus_tag="VFE39_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HET6259636.1"
                     /translation="MNLLRPLRLLVPFVAIAAALGLFLGLGFIVFSTGSGGGRPPTYG
                     PCDAGALSSSGSLGRGRAGTRIDDLSDEQRRNAATIIGVARDLGAPPRAWLVALTTAM
                     QESTLRNINYGDRDSLGLFQQRPSQGWGSPAQVTDPVYSTRMFIQRLLAVPGWMNLPV
                     TVAAQTVQRSAFPDAYAKWEGLAAQLVQQLADVADPAGCGRTAVLPDGVASTAIAFAL
                     GELGKPYVWGATGPNTYDCSGLMLRAFQAAGINLPRVSREQFYAGGHVPISQAQPGDL
                     LFYATDPSDPDTIHHVVLYMGNGQMVEAPYTGQSVRVRPVPWHYAELVPLATRPGTTP
                     NRA"
     gene            complement(1443..1787)
                     /locus_tag="VFE39_13385"
     CDS             complement(1443..1787)
                     /locus_tag="VFE39_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259637.1"
                     /translation="MPDLEPTTLPLSQAPAAALSVAARWSAAWVRPPDGTTAQQWLDG
                     LRSTTTEEYLGVLSNVDPGNIPATRVTGEPRAVRVAPRSIQVQVPTDALTLIVLVVDT
                     ETGWRVAGYDRA"
     gene            complement(2047..3966)
                     /locus_tag="VFE39_13390"
     CDS             complement(2047..3966)
                     /locus_tag="VFE39_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259638.1"
                     /translation="MPEGLPPVMGWLPVALVLAAAVLVDARRRRRARTDARPRRRVAL
                     MIVVGLVGGSVLIGQPAFAQSLDCKESPEPDRPGTGLVGSLDPPTYGVGEPGSVYDEV
                     GYAGLVWYNYDLGCAGAAVFNPATTTDTWLGNQTFNGAKFVVAGVNWSHYLIADGGEL
                     LSPLDQLIGDGTRAMYTAVFTTWIGPALLVLAVLLLILALRGDLARQTQRAAFAVIAL
                     VVGSAAYLAPVDWAKAADPLLLNGVNDMQQGFLSQVGLGDRDTLPTVLVDQVIYRNWL
                     RGEFGSPDVPQAQELGRDLLRAQTFTKPEVAEGRDTLQLGEQKKADFATIAGRMGDRY
                     SYFQGKSGSRIGAGILAVVQALCIALFQLLSKVLVLVAMLLLRLLVMTAPAVAVVAVL
                     KPDILPSLLRVAGAAIVNSLVVGALAGLHALLVVSLFRPGSGVDLWLALLVTGVVTVV
                     LWAVARPFRRLVSMVSLTREQFGGIVPGAGTGPMSRVWQRMRGGPAEDRQTRWWDERR
                     GAASGYGYPDGERPEAEPGIRIPPQSGVRPVRRRAETEAAPSPTLVPAARRPALPAGP
                     GGVGSGASTPSWADPGEVDERVIYRRPDAVPLRPGGARPVHAELVDGVPVYRIYRPRS
                     TPAAYSPSGGPRRAD"
     gene            complement(3966..7031)
                     /locus_tag="VFE39_13395"
     CDS             complement(3966..7031)
                     /locus_tag="VFE39_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6259639.1"
                     /translation="MARRDAARDRSSVEGQRGRRRGRALAGEAGLPKYVPEIALRSID
                     GHLTRTGTQVMAWYRLAAQAWSFRSDSQREVLIRQIAAQLGELQGRWLHLRVTTRPYP
                     VHMWAESFDHNALGRMPDVAGALGWDGFLEGEQRHLMGLSMSDKEVFLGVEVSGRSVL
                     DRWVERAAPVLGRVAPAAVRAELAALSSEVGHLDALVAGSGLDAVPASADDIAWLMHR
                     SCALGLPAPRTLSVVPSGVNRWETEDLAAFTDGVDMHQEPYAPTVRVIGRLRTQTVSR
                     NVAVLSLGLMEGLRIPEVDDPWMQHSDRLPFPVEWSARIYVRRPEDVAGELQRQMGKV
                     RSQIRHYTHDHDLDPPMSLARQADRVLEVEDELTSGLTQLNTRLYGWWRVAVSGKDEA
                     EAVGRAQQVLDVYRPKVQIEHPEAQYRYAREFIPGEPLASTAYRRRGSVTWAAAAVPA
                     ATASVGDRRGIMIGETCTATRRPVAWDPWLAQEIRRASGLTAVVGGLGSGKSFLTGVI
                     VYKTLRAGAQWTVLDPSGPLAELTRLPELAPFSRHINLLRADPGILNPYRVVAEPRPE
                     HFLDEEDPERAWRRERSLAAATRRRLVLDVLTGLLPYDVARLPHTRIVLLRAVREVGG
                     ATDRHPGQVIDALRRHARDGQEHAGVVADFLEERRELPQAALLFPDTSRHDPWQADRE
                     YRLTVLTMQGMTLPRPGSPREEWTDGESLAVELLNLASWLTQRTIYDADRNLRKGVAL
                     DETHFLSQVPTGKVLIDRLARDSRKFNVRALFASQLAGDLLRVSGFASLVNAVFVGRT
                     DDDEAQADALRLLKVPTGVGYEQMLGTLSPRPRHDDRPDDTPRQFIFADGHGGVEKIR
                     IDLEAPHLEHVRDALDTNPDASRVLARGVPAATAHTAVRAPDEPAPPGPVALDHILGG
                     GNRLRREYELVGDDLDEEFADLDDPDGLLAVDAGEQQPGGSTSSATNELNELLDDEEL
                     DLLGDVDSDGDPLDQHTIDDDDDGDDDNDNGGPVHRGSERVRTVGEARR"
     gene            complement(7018..7479)
                     /locus_tag="VFE39_13400"
     CDS             complement(7018..7479)
                     /locus_tag="VFE39_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259640.1"
                     /translation="MLVRTDDEIYRVDSVWLGPPRATFPWRARYVSYGVGLLVMIGIM
                     FLQRRVGIGLDFFSVAWALVITVVITRFLGQRINYERPLGQVVELFRHEITGPRARTA
                     VGGGEVRSGHVRVGPVVRVRKASADSASWQRSQTESRAVAGRRKGRSRGKA"
     gene            complement(7484..7744)
                     /locus_tag="VFE39_13405"
     CDS             complement(7484..7744)
                     /locus_tag="VFE39_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259641.1"
                     /translation="MTGPEVLALYAQGLSTNGLQGWIQNNVVPLVLLGIAIILLWIGG
                     RGDNAGVARRSVGLLIGLVALGIAVSGNGPEIGNFLAGLLVG"
     gene            8176..10620
                     /locus_tag="VFE39_13410"
     CDS             8176..10620
                     /locus_tag="VFE39_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HET6259642.1"
                     /translation="MVVRLVIGLAMTLIAVAVTGRRALWLYTLIKSGQPAHDRTDGLN
                     QRIRAQFVEVFGQRKLLKWSVPGLAHFFTFWAFVILITVYLEAYGALFDHNFHIPLIG
                     RWPVLGFLQDFIAAAALISLGVFSVIRLRNAPQRRARASRFYGSHTGGAWLILFMIFN
                     VLWTMFFFRGASSALGVFPYRSGAFVSIGVGHLFDGVSEGTLQVLESVGLLLHIGVML
                     VFTVIIAYSKHLHIFTAPINVSAKRFPKALGPLPVMESNGKPIDFEDPGEDDVFGRGK
                     VEDFTWKGMLDFATCTECGRCQSQCPAWNTGKPLSPKLVIMDLRDHLLAKAPYIIGGK
                     DMPEEGAVSVSGGADQAGGHGHHVPESGFGRVPGSGPEQAMRPLVGTLEQGGVIDPDV
                     LWSCTTCGACVEQCPVDIEHVDHIVDMRRHQVLIESEFPSELGVLFRNLENKGNPWGQ
                     NAKDRLDWTKNLDFEVPVFGADGSGELSADTEYLFWIGCAGAFDDNAKRTVRATAELL
                     HRAGVGYVVLGPEESCTGDPARRSGNEFLFQMLAQQNVEMLNAVFEGREPGTRKIVTT
                     CPHCLNTLGREYPQLSQDGIGGHYEVVHHTQLLNKLVREGKLVPVAAPDGAMADVTYH
                     DPCYLGRHNEIYEEPRELVGAAGVKLTEMPRHADRSFCCGAGGARMWMEEKIGKRVNL
                     ERVDEALGTGAEKIATGCPFCRVMFSDGLTQRQSEDKGTGVEILDVSQMLLAAVQRGD
                     AARDGDANGKVDALGAGSPHTADTTPTSGTQVNGPGDESAVNADPAGDVSVGRAGTGG
                     AEPKGATSRDEPAGGN"
     gene            complement(10648..11547)
                     /locus_tag="VFE39_13415"
     CDS             complement(10648..11547)
                     /locus_tag="VFE39_13415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259643.1"
                     /translation="MEPPRPTPPPWLPAARPQVPPPAVPDPLPTPPPAPPARPRFDWR
                     RLTRSPRGAVGLAITAAALLLWPFSGLSWIPWLVGLGALVVLRILRLDGLLRGWDLHI
                     AGLVVVVGLMMSTSPWAWALAASIGVLLAGLAQLPWWRLAAVGAVLCLVSGLAFAWAN
                     VRDAREAAASYGMLQQESKADQGAPRPQSVLPLLLNRIALGSPGAICDNLLAEPARTQ
                     FAASVGQPDCTAAVMALAARVSDPVRYAQADVTTTPTATGLEVDACSMDWGSRAPAGP
                     QVGHLTIGAVAPSRYVVTGFRPC"
     gene            complement(11600..12550)
                     /locus_tag="VFE39_13420"
     CDS             complement(11600..12550)
                     /locus_tag="VFE39_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET6259644.1"
                     /translation="MGAVGRSSVLPTVGTLDRGGRAIPVRTYDRLPGVPPVRVLWSGG
                     GRHADGFGSLHPHAHDFLVLLYVERGGSSMRVDDREWALGTGDVFVVAPGEVVTPDGV
                     VGAEAPEAWAVAFPPDIIEPSLPGSFLSWRAHPLLFPFVRGVAGGAQRLHVPVDDRAG
                     ISERFAALERELHERRDGYNEAVLAHLTLLLVAVSRLASDVASDLTLRDEPMLAAVFD
                     VIEASYHRPISLKDVASAVGLTPGHLTTIVGRKTGRTVQQWITERRMTEARRLLAETD
                     VTVEAIGARVGYRDTSYFIKRFRRSNGSAPQEWRRAGRRG"
     gene            12637..13281
                     /locus_tag="VFE39_13425"
     CDS             12637..13281
                     /locus_tag="VFE39_13425"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6259645.1"
                     /translation="MTEQVPQHTGHGHGYLPAMGHDRLLRFYDPFTRLLGVRSLHRTL
                     ADQADIAPGHRVLEIGCGTGNLALLVAHRYRGATVVGLDPDPLALARARRKATRDGLP
                     VQWDLGTAGDLPYPDASVDRVLSALMFHHLDEAEKHRALSEIKRVLRPGGRLHLVDFG
                     GNHRGLLARLTRRNPRLQDNAGDRIPARMREAGLTDVGELGRGRLDVTFYRATA"
     gene            13382..13735
                     /locus_tag="VFE39_13430"
     CDS             13382..13735
                     /locus_tag="VFE39_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259646.1"
                     /translation="MLAVLGGLALVVTATLATSVVTPSPERAQITTLPLMFVMLGVGV
                     VGAIAPGQGWWSVLMVVPGAAVGDLAQLAMTGGSWSAVTAGLPAILPPLVALEVWPVV
                     FWVLAARGFRWDPRH"
     gene            complement(13755..14336)
                     /gene="dcd"
                     /locus_tag="VFE39_13435"
     CDS             complement(13755..14336)
                     /gene="dcd"
                     /locus_tag="VFE39_13435"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HET6259647.1"
                     /translation="MLLSDGDLRKELEAGRLVLEPWDPAMLQPSSIDVRLDRFFRVFQ
                     NSRYTHIDPAQQQDELTSAVEPDGDDPFVLHPGEFVLGSTFESVSLPDDLAGRLEGKS
                     SLGRLGLLTHSTAGFIDPGFTGHITLELSNVANLPITLWPGMKIGQLCLFRLSSPAER
                     PYGSHGVGSRYQGQRGPTPSRAHLNFHRADTRR"
     gene            complement(14360..15514)
                     /locus_tag="VFE39_13440"
     CDS             complement(14360..15514)
                     /locus_tag="VFE39_13440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HET6259648.1"
                     /translation="MTELDQPMGSLPDQDHDHDVLLIAGSRPEVARLAPVATAFANAD
                     RIRALTVATGPDPMTVHEAFESLGVPADVTQLLREPPGPQPAAIASLLMTRLDELLVD
                     LDPSAVMVYGGGMTAVVAAQVAFWRQVPVVHLQAGVATDDLLCPFPQEANRRVIGQLA
                     SLFLTTTGDATLGSRVGPNAISVGDTMAANPQPADLRFARLIRRVRAGGPQVVLVGLD
                     RPDSLGVLAGLPDLLDREPDIEIVVYGELASHGAAYPLLQHPRAIVVRNLPLAELVWL
                     IAASAVLVSDDPELVIDAPGLGTPAVLVDGPHIPEPGDSIRSILSPAVMTTVRQVLVA
                     TPKLAPAPSDGLEAVRVEQAVAWMFGLCPSPLLTPNLPFSANDSATNTEV"
     gene            15700..15770
                     /locus_tag="VFE39_13445"
     tRNA            15700..15770
                     /locus_tag="VFE39_13445"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15732..15734,aa:Gly,seq:ccc)
     gene            complement(15862..16134)
                     /locus_tag="VFE39_13450"
     CDS             complement(15862..16134)
                     /locus_tag="VFE39_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259649.1"
                     /translation="MKKLGRPASAATSCGTREPLAAQTGASTKDLMSRMGHDNMRALI
                     DQHATSQADRLIADRQSGLLDEHRRTRRKSPEGEEDGPAGHLVPVS"
     gene            16274..16624
                     /locus_tag="VFE39_13455"
     CDS             16274..16624
                     /locus_tag="VFE39_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259650.1"
                     /translation="MPSVLVLGFGPQLVPGADGDGVLAVLDRELARFGEHDIDASVTL
                     IAPDRTAETTVVAASAAQPWDVVVVGGGIRKPEPVLTLFEKLVNLIRQHAPQAAILFN
                     TSPADTVEAALRWL"
     gene            complement(16897..18096)
                     /gene="dinB"
                     /locus_tag="VFE39_13460"
     CDS             complement(16897..18096)
                     /gene="dinB"
                     /locus_tag="VFE39_13460"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET6259651.1"
                     /translation="MFVSREGTILHADLDAFYASVEQRDDPRLRGRPVIVGGGVVLAA
                     SYEAKAYGVATAMGGTQARSLCPHAIVVPPRMSAYSAASKAVFEVFRNTTPLVEGMSI
                     DEAFLDVGGLGRIAGPPSGIAARLRADVLERVGLPITVGVARTKFLAKVASGVAKPDG
                     LLVVPPEDELGFLHPLAVERLWGVGKVTAAKLREHGISTVGEVAELPENILVSILGRA
                     LGRHLHALAHNLDPRPVQVGRRRRSIGAQRALGRRAPTTPAAIDAVAVALVDRIARRL
                     RTAGRVGRTVVLRLRLADYSRLTRSHTLPQPTCATEAILTTVRVLVARAMPLIEERGL
                     TLVGVAVGNLDDDDAVQLVLPFDEDAGAALDAVLDTLHDRFGSAAITRAVLLGRDPGV
                     TVPLLPE"
     gene            complement(18202..18543)
                     /locus_tag="VFE39_13465"
     CDS             complement(18202..18543)
                     /locus_tag="VFE39_13465"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6259652.1"
                     /translation="MTHGVTIRVAGPRVSFLAEAGGRLVGMMHLVVFERMPSPERAPT
                     RWGYLANAFVLERHRNRGIGSALLDQLVGHAREIGCVRLVLSPSERSVPFYARAGFGP
                     ATMLMAQVLES"
     gene            complement(18548..19042)
                     /locus_tag="VFE39_13470"
     CDS             complement(18548..19042)
                     /locus_tag="VFE39_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6259653.1"
                     /translation="MNTTTTPLLVRASDAETLTGDPGGTIALLADSVSTGGAITCNRS
                     TFRAGADGAPPHFHTRASELFVVLGGSLQVLAGDRLHILDQGDVLLVPPHVPHAFGAA
                     AGVDADVLVVFTPGMDRFDYYRLLDRVHRGDAEPREIGESQERFDNHYVDISLWREAR
                     TTAR"
     gene            19114..20037
                     /locus_tag="VFE39_13475"
     CDS             19114..20037
                     /locus_tag="VFE39_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6259654.1"
                     /translation="MEMREIEIFLALADELHFGRAAERLRVSQARVSQTIKKLERQVG
                     APLFDRTSRRVALTPIGARLRDDLAPAHRRIIDGVTRATDAGRGQGGSLRLAFEAPGV
                     ADLIAEVLDAYRFCSPDSDVQVREADFRDPFGLLRGGEVDALVTLLPVAEPDLTTGPV
                     VLTEPVVLAVPVRHPFARLDSVTLDDLARDTVFRAARPAPPYWVEPPQPWATPGGHPV
                     QRGRPLTTFQELLAVIAGGEGICPLAAHATQYFAHPKIAFVPFRDAPHVEWALVWRRA
                     GETGRVRELARCIRTGAGRTVATRAERRERL"
     gene            complement(20038..20385)
                     /locus_tag="VFE39_13480"
     CDS             complement(20038..20385)
                     /locus_tag="VFE39_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET6259655.1"
                     /translation="MPTWDDVVAIAVALPEVEQSTSYRTPSLTVQGKSFARLRTEAEG
                     GLVLMCQIDEKEALLASGDPAFYTTPHYDGYGAILVDLEKVSHQQLTELIEDAWRDRA
                     PVRVRKAFDAERS"
     gene            complement(20435..21253)
                     /locus_tag="VFE39_13485"
     CDS             complement(20435..21253)
                     /locus_tag="VFE39_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259656.1"
                     /translation="MLVGILLATVAMVLNTFGALLQAEGARRATRTRPAAIQPRYIAG
                     VAVDFLAWGCAVLALRVLPVFAVQAIIGGTIALTAVFNARMLGVRLPVRTRVAVGVCL
                     TGLVMVAASAGSHHPPVRTRGVDVILLVSLLLLGVAVLVLRQGKHSWPLALVAGLGFG
                     GTALSVRAAHVQTGERFDLVPLLGQPATYLVVGFWLVGMVGYTAALSRGDVGPVTAVF
                     TVTEVLVPGMVGIQLLDDPVRPGWAWVLAVGLAAALAGTVVLAKAPPLRPPRVR"
     gene            complement(21314..21784)
                     /locus_tag="VFE39_13490"
     CDS             complement(21314..21784)
                     /locus_tag="VFE39_13490"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6259657.1"
                     /translation="MLIRPARPDEAAFLAALAVRSKGHWPYPAAFLARFARTLALTPD
                     VVAANDVNVGERAGEVRGFYTLLHRGALAVLDDLWLEPAEIGRGSGRLLFEHAVARAT
                     AAGSTVLEWEAEPYATGFYERMGGVTAGWTDSPLGRRMPVMRLPLSRGPAFSAV"
     gene            complement(21813..22085)
                     /locus_tag="VFE39_13495"
     CDS             complement(21813..22085)
                     /locus_tag="VFE39_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259658.1"
                     /translation="MVRLGGAVIAMEIAALGVAYVAFGWLVLLVGAPLVLGTVLVLAF
                     VRASEGPRRKLHEHRAERWDARSAVAGGLMSGFFTPVASGEDRGGR"
BASE COUNT         3501 a         7976 c         8137 g         3172 t
ORIGIN      
        1 ggtcggcgac cggcgccagc acccggcggt tctccgggtc ggccaccacg ttgtcggcgc
       61 tgtcgcggta ctccagctgc ccgtcctggc cgaggtcggc gacgtatccc gcatcggcga
      121 gctgctcctc gtcgaggtcg accagccgct cccagcggct cggcaggacc ctgtagtccg
      181 gccaggccag gtggccatag gcgtcgtgca ggtcggcgag cagcccgacg aacacctgct
      241 gccagcgcag cggcatcgac tgggccagtg accgcggaac cacgagatgg tcctcggtgg
      301 cgccacgagc gggccggtcg aggtagtcct gcaccggcgt cgtggacgcc gggacggagg
      361 gttcgggggg cattgctact cctgctcgta catggacgcc gacctcgacc ctaggcgcgg
      421 ttcggtgtgg tgccgggacg cgtggcgagc ggcacgagct ctgcatagtg ccacggcacc
      481 ggccgcaccc tgacggattg ccccgtgtag ggcgcctcca ccatctgccc gttccccatg
      541 tacagcacaa catggtggat cgtgtccgga tccgacgggt cggtggcgta gaacagcagg
      601 tcacctggct gagcctgaga gatcggaacg tggccgccgg cgtagaactg ctccctcgac
      661 actctcggca ggttgattcc ggcggcctgg aaagcgcgca gcatcagccc ggagcagtcg
      721 taggtgttcg ggccggtcgc gccccatacg tagggctttc cgagctctcc gagtgcgaag
      781 gcgatcgccg tgctcgcaac gccgtccggg agcaccgcgg tccgcccgca gccggccggg
      841 tcggccacgt cggccagctg ctgcaccagc tgggcagcga ggccctccca cttcgcgtag
      901 gcgtcgggga aggccgagcg ctgcacggtc tgcgccgcca ccgtgaccgg gaggttcatc
      961 cagccgggca ccgccaggag ccgctggatg aacatcctcg tgctgtagac ggggtcggtg
     1021 acctgggccg gcgatcccca gccctgggag ggacgctgct ggaagagccc gagggagtcc
     1081 cggtcgccgt agttgatgtt gcgaagggtg gactcctgca tggccgtggt cagcgcgacg
     1141 agccatgcac gcggcggagc cccgaggtcc cgggcgactc cgatgatggt ggcggcgttg
     1201 cggcgctgct catcgctcag gtcatcgatc cgtgtccctg ccctgccccg cccgagcgat
     1261 cccgatgagg acagcgcccc ggcatcacac ggtccgtacg tgggcggccg tccacccccg
     1321 ctgcccgtgc tgaacacgat gaagccgagg ccgaggaaaa gccccagcgc agccgcgatc
     1381 gcgacgaacg gtacgaggag ccgcagcggc cgcagcaggt tcatcgcgtg cgcgcgccgc
     1441 cgtcaggccc ggtcgtagcc ggcgacccgc cagccggtct cggtgtcgac gacgagcacg
     1501 atcagggtga gcgcgtccgt cggcacctgc acctgaatcg aacgcggggc gacccgcacg
     1561 gcccgcggct ccccggtaac ccgggtggcc gggatgttgc cggggtcgac gttgctgaga
     1621 accccgaggt actcctccgt ggtggtggac cgcaggccgt cgagccactg ctgggcggtg
     1681 gtgccgtccg ggggccggac ccacgcagct gaccaacgcg ccgccaccga gagcgcggcg
     1741 gcaggggcct gcgacagcgg cagggtggtc ggctcgaggt cgggcacggg aggtagcgct
     1801 gtcggggcag gtgtggacgg gacggctgcg gatcccggag cacggggtac agaactccca
     1861 gaggccgagg acggccacgg gcctgccgtg ccccggccgt ccgagccgcc gccgagtgca
     1921 ccgatggcga gactcacccc gacagccacg gcgacgacga ccaccgcggt gaccgcaagc
     1981 cgggcaggcg accgcagggg ccactgccag attccgcggt aggcggcggc ccgcccgcga
     2041 ggggttctaa tcggcacggc ggggcccgcc gctcgggctg taggccgccg gagtcgagcg
     2101 cggccggtag atgcgataca ccggtacgcc atcgacgagc tcggcgtgca ccggacgcgc
     2161 gccaccaggg cgcaacggca cggcgtccgg gcggcggtag atcacgcgtt cgtcgacctc
     2221 gccgggatcg gcccaactcg gcgtgctcgc accgctgccg acgccaccgg gcccggcggg
     2281 aagcgcgggc cggcgtgctg cgggaacgag cgtcggagac ggggcagcct cggtctccgc
     2341 gcgccgacgt accggccgca cacccgactg cggggggatc cggatgcccg gctcggcctc
     2401 gggacgctcg ccgtccgggt atccgtatcc cgacgccgcc ccgcgccgct catcccacca
     2461 ccgggtctgc cggtcctcgg cgggcccgcc gcgcatccgc tgccagacgc gggacatggg
     2521 cccggtgcct gcgcccggca cgatcccgcc gaactgctcg cgggtgagcg acaccatcga
     2581 cacgagtcgc cggaacgggc gggcgacggc ccacagcacc acggtcacca cgcccgtgac
     2641 gagcagggcg agccacaggt cgacgccgct gcccggccgg aacaacgaca cgaccagtaa
     2701 cgcgtgcagt ccggcgagcg cccccaccac gaggctgttc acgatcgcgg cgccggcgac
     2761 gcgcagcagc gacgggagga tgtcgggctt gagaacggcc accacggcga ccgcgggcgc
     2821 ggtcatcacc agcagccgca gcagcagcat cgcgaccagc acgagcacct tggacagcag
     2881 ctggaacaga gcaatgcaca gcgcctgcac cacggccagg atgccggcgc cgatacggct
     2941 gcccgacttg ccctggaagt aggagtagcg gtcgcccatc cggccggcga tcgtggcgaa
     3001 gtcggccttc ttctgctcgc cgagctgcag cgtgtcgcgg ccctcggcga cctccggctt
     3061 cgtgaaggtc tgcgcacgca gcaggtcacg acccagctcc tgcgcctgcg gcacgtcggg
     3121 ggaaccgaac tcgccgcgca gccagttccg gtagatcacc tggtcgacga gcacggtcgg
     3181 gagggtgtcc cggtcaccga gccccacctg gctgaggaag ccctgctgca tgtcgttgac
     3241 gccgttgagc agcagcgggt cggccgcttt cgcccagtcg accggagcca ggtacgccgc
     3301 tgagccgacc acgagggcga tcaccgcgaa cgcggcacgc tgagtctgcc gagcaaggtc
     3361 cccacgcagc gcgaggatca ggagcagcac ggccagaacg agtagcgcag ggccgatcca
     3421 cgtggtgaac accgccgtgt acatcgcccg ggtgccgtcg ccgatcagct ggtcgagcgg
     3481 ggagagcagc tcaccgccgt ccgcaatgag gtagtgcgac cagttgacgc cggcgaccac
     3541 gaacttcgcg ccgttgaacg tctggttgcc cagccaggtg tcggtggtcg tggccgggtt
     3601 gaagacggcc gctccggcgc agccgaggtc gtagttgtac cagacgaggc ccgcgtaacc
     3661 cacctcgtcg tagacgctgc ctggctcacc cactccatag gtcggcgggt cgagcgaacc
     3721 gaccagaccc gtgcccggcc ggtctggctc cggcgactcc ttgcagtcga gcgactgcgc
     3781 gaaggcgggc tgtccgatca gcaccgagcc cccgacgagc ccgaccacga tcatcagcgc
     3841 cacccggcgt cggggcctgg cgtcggtgcg cgcccggcgc cgacgacgcg cgtccaccag
     3901 caccgcggcg gccagcacca gcgccaccgg cagccatccc atgaccggcg gcagcccctc
     3961 gggcatcacc gacgggcctc cccgaccgtg cgcactcgtt cgctgccacg gtggacaggg
     4021 ccgccgttgt cgttgtcgtc gtcgccgtcg tcgtcgtcgt cgatcgtgtg ctggtcgagc
     4081 gggtcgccgt cgctgtcgac gtcgccgagg agatcgagct cctcgtcatc gagaagttcg
     4141 ttcagttcgt tcgtcgcgct gctggtcgag ccaccaggtt gttgttcccc ggcgtcgacc
     4201 gccaacagcc catcaggatc gtcgaggtcg gcgaactcct cgtcgaggtc gtcgccgacg
     4261 agctcgtact cgcggcggag ccggttgccg ccgcccagta tgtggtccag tgccacgggt
     4321 ccgggtgggg cgggttcgtc gggagcccga accgccgtgt gagcggtagc cgccggaacg
     4381 ccgcgggcaa ggacccggct ggcgtccggg ttggtatcga gggcgtcgcg gacgtgctcc
     4441 aggtgcggcg cctccaggtc gatacggatc ttctccacgc cgccgtgccc gtcggcgaag
     4501 atgaactgcc gcggagtgtc gtcgggccgg tcgtcgtgcc gaggccgcgg cgacagcgtg
     4561 ccgagcatct gctcgtagcc gacgcccgtg ggcaccttca gcagccgcag cgcatcggcc
     4621 tgtgcctcgt cgtcgtcggt gcggccgacg aacacggcgt tgacgagcga cgcgaacccg
     4681 gagacccgca gcaggtcacc ggcgagctgc gacgcgaaca gcgcacggac gttgaacttg
     4741 cgcgagtcgc gggcgagccg gtcgatgagc accttgccgg tgggcacctg cgagaggaag
     4801 tgggtctcgt cgagcgcgac acccttccgg aggttgcggt cggcgtcgta gatcgtgcgc
     4861 tgggtgagcc aggacgccag gttcagcaac tcgacggcga gcgactcacc gtcggtccac
     4921 tcctcgcgcg ggctaccggg ccggggcagc gtcatcccct gcatcgtgag aacggtgagg
     4981 cggtactccc ggtcggcctg ccaggggtcg tggcgggacg tgtcggggaa cagcagcgcc
     5041 gcctgtggca gctccctgcg ctcctccagg aagtcggcga cgacgccggc gtgctcctgg
     5101 ccgtcacgcg cgtgccggcg caaggcgtcg atcacctggc cgggatgccg gtcggtggcc
     5161 cctccgacct cccgaaccgc gcggagcagc acgatgcggg tgtgcggcag ccgagcgacg
     5221 tcgtagggga gcagcccggt gagcacgtcg aggacgaggc gtcgtctcgt ggctgccgcg
     5281 agggagcgct cacggcgcca cgcccgctcg gggtcctcct cgtcgaggaa atgctccggc
     5341 cgcggctcgg cgaccacccg gtacgggttg aggatgccgg ggtcggccct gagcaggttg
     5401 atgtggcgtg agaacggggc gagctcgggc agccgcgtga gctcggcgag cgggcccgac
     5461 gggtcgagca cggtccactg ggccccggcc cgcaacgtct tgtagacgat cactccggtc
     5521 aggaaggact tgcccgaccc gagcccaccg acgacagcgg tgaggccgga cgcccttcgg
     5581 atctcctgcg cgagccaggg atcccaggcc accgggcgcc gggtggccgt gcacgtctcg
     5641 ccgatcatga tgccgcgccg atcgccgacg gacgcggtgg cggcgggcac tgcggctgcg
     5701 gcccaggtga cggacccgcg gcgccggtag gcggtggacg cgagaggctc gccggggatg
     5761 aactcccggg cgtagcggta ctgcgcctcc gggtgctcaa tctgcacctt cggccggtac
     5821 acgtcgagca cctgctgcgc cctgccgacc gcctccgcct catccttgcc ggacacggcg
     5881 acccgccacc acccgtagag ccgggtgttg agctgggtga gcccgctggt cagctcgtcc
     5941 tcgacctcca gcacgcggtc ggcctgccgg gccagggaca tcggggggtc gaggtcgtgg
     6001 tcgtgggtgt agtgccggat ctggctgcgc accttgccca tctggcgttg cagctcaccg
     6061 gccacgtcct cgggacgccg cacatagatc cgcgccgacc actcgaccgg gaacgggagc
     6121 cggtcggaat gctgcatcca cgggtcgtcg acctcgggga tgcgcaggcc ctccatgaga
     6181 ccgagggaca gcacggcgac gttgcgcgag actgtctgcg tgcgcagccg cccgatcacg
     6241 cgaacggtgg gagcgtaggg ctcctggtgc atgtcgacgc cgtcggtgaa ggccgcgagg
     6301 tcctccgtct cccacctgtt gacgccgctg ggcaccaccg agagcgtccg cggggcgggc
     6361 agtcctagtg cgcacgaccg gtgcatcaac caggcgatgt cgtcggccga agcaggcact
     6421 gcgtccagcc cgctaccggc gacgagcgcg tcgaggtgcc cgacctcgct ggacagcgcg
     6481 gcgagctcgg cgcgcacggc ggccggcgcc accctgccca gcaccggagc cgcccgctcg
     6541 acccagcggt cgagcacgct ccgcccggag acctcgacgc cgaggaacac ctccttgtcc
     6601 gacatcgaca ggcccatcag gtggcgttgc tcaccctcca ggaacccgtc ccaacccagg
     6661 gccccggcga catccggcat ccggccgagc gcgttgtggt cgaacgactc ggcccacatg
     6721 tggacgggat agggccgggt ggtgacgcga agatgcagcc accggccctg cagctctccg
     6781 agttgcgcgg cgatctggcg gatcagcacc tcgcgctgtg agtcgctgcg gaagctccag
     6841 gcctgcgcgg cgaggcggta ccaggccatc acctgggtgc cggtgcgggt cagatgtcca
     6901 tcgatcgacc gcagtgcgat ctcggggacg tacttgggca acccggcctc gcctgcgagc
     6961 gcacggccgc gacgcctgcc gcgctgcccc tcgacggagc ttctgtcgcg agcggcgtca
     7021 cgccttgcca cgtgaacgtc ccttccggcg cccggctact gcccggctct ccgtctggct
     7081 gcgctgccaa gaggcactgt cggctgacgc cttgcgaacc cggacgacgg ggccgacccg
     7141 cacgtggccg gaacggacct ccccgccgcc gactgccgta cgcgccctcg gcccggtgat
     7201 ctcgtgacgg aagagctcca caacctgccc gaggggtcgt tcgtagttga tgcgctggcc
     7261 gaggaagcgg gtgatcacga ccgtgatgac gagtgcccag gcgacggaga agaagtcgag
     7321 cccgatcccg acgcgccgct gcaggaacat gatgccgatc atgacgagca ggccgacgcc
     7381 atagctgacg taccgcgcgc gccacgggaa cgtggcgcgc ggtggaccca accagacgga
     7441 gtcgacgcgg tagatctcgt cgtcggtgcg gacgagcaac tgatcacccg acgaggaggc
     7501 cggccaggaa gttgccgatc tccggaccgt ttcccgagac ggcgatgccg agcgcgacaa
     7561 gtccgatgag cagacccacg ctgcggcggg ccacgcccgc gttgtcaccg cgaccaccga
     7621 tccacagcag gatgatcgcg ataccgagca ggacaagagg gacgacgttg ttctggatcc
     7681 acccctggag accgttggtg ctcaggccct gcgcatacag cgcaagcacc tctggtccgg
     7741 tcatacggct cctccttgcg gcgctaccca cccgaggctg cccgtaccga ccccccgggc
     7801 tggttgatac ccgaacgtgt cgactcggct atccagcgtt gagtagacca tgctgaccag
     7861 cgggagcata gagggtcgcc ctcacggcgg gcacgaggtc gcacgaagtg ccatggacac
     7921 cgccgacggg gtgactgacc tggggaacgt tgggcgtccc actgtctcca ctgatccagt
     7981 gaagaccacc ggagtgaccg ttcggtgatc ggtgcgggcc gactccgggt cccggggcgc
     8041 gccggttgtt accgacgggt aggttaggcc cctgaacggc ccgcatgttt ccggtagcga
     8101 gcgggagcgg cggtctaccc tgctccgaaa cggcccgcgg ttcaccgctc gcgcgccagc
     8161 tctgggagga agcccgtggt tgtcagactg gtcatcggac tggcgatgac gttgatcgcc
     8221 gtggcggtaa ccggccgccg cgcgttgtgg ctctacacgt tgatcaagtc ggggcagccg
     8281 gctcacgacc gcaccgacgg gctgaaccag cgcatccggg cccagttcgt ggaggtcttc
     8341 ggccagcgca agctactcaa gtggtcggta cccgggctgg cccacttctt cacgttctgg
     8401 gcgttcgtga tcttgatcac ggtgtacctc gaggcgtacg gcgcgctgtt cgaccacaac
     8461 ttccacatcc cgcttatcgg acgctggccg gtgctcgggt tcctgcagga cttcatcgct
     8521 gctgcggcgc tgatctccct cggggtcttc tcggtgatcc ggctgcgcaa cgccccgcag
     8581 cgccgtgcgc gggcgtcgcg gttctacggc tcccacaccg gcggggcgtg gctgatcctg
     8641 ttcatgatct tcaacgtgct gtggacgatg ttcttcttcc gtggcgcgtc ctcggccctg
     8701 ggggtcttcc cgtaccggtc gggggcgttt gtctcgatcg gtgtcggcca tctgttcgac
     8761 ggcgtgtccg agggcacgct gcaggtgctg gagagcgtcg ggctgttgct gcacatcggc
     8821 gtgatgctgg tcttcacggt gatcatcgcc tacagcaagc acctgcacat cttcaccgcg
     8881 ccgatcaatg tctcggccaa gcggttcccg aaggcgctgg gtccgcttcc ggtgatggag
     8941 tcgaacggca agccgatcga cttcgaggac cctggcgagg acgacgtgtt cggccgcggc
     9001 aaggtcgagg acttcacgtg gaagggcatg ctggacttcg cgacctgcac cgagtgcggc
     9061 cgctgccaga gccagtgccc ggcgtggaac acgggcaagc cgctgtcgcc gaagctcgtg
     9121 atcatggacc tgcgggacca cctgctggcg aaggcgccgt acatcatcgg cgggaaagac
     9181 atgcccgagg agggcgcggt ctccgtgtcg ggcggtgcgg accaggcagg cggccacggc
     9241 caccacgtcc cggagtcggg cttcgggcgg gtgccgggct ccggcccgga gcaggcaatg
     9301 cgcccgctgg tcggcacgct ggagcagggc ggcgtgatcg accccgacgt gctgtggtcc
     9361 tgcaccacct gcggggcgtg cgtcgagcag tgcccggtgg acatcgagca cgtcgaccac
     9421 atcgtcgaca tgcgtcggca ccaggtgctg atcgagtcgg agttcccgtc cgagctcggg
     9481 gtgctgttcc gcaacctgga gaacaagggc aacccctggg gacagaacgc caaggaccgc
     9541 ctcgactgga ccaagaacct cgacttcgag gtgccggtct tcggtgctga cgggtcgggc
     9601 gagctgtccg ccgacacgga gtacctgttc tggatcggct gcgcgggcgc gttcgacgac
     9661 aacgccaaga ggacggtgcg ggccaccgcg gagctgctgc accgggcggg cgtcggctac
     9721 gtcgtgctgg gcccggagga gagctgcacc ggtgacccgg cgcgccgctc gggcaacgag
     9781 ttcctgttcc agatgctcgc ccagcagaac gtcgagatgc tcaacgcggt cttcgagggc
     9841 cgcgaaccgg gcacccggaa gatcgtgacg acctgcccgc actgcctcaa caccctcggt
     9901 cgggagtacc cgcagctcag ccaggacggg atcgggggcc actacgaggt ggtgcaccac
     9961 acccagctgc tcaacaagct ggtgcgtgag ggcaagctcg ttccggtcgc cgcacccgac
    10021 ggcgcgatgg ccgacgtcac ctaccacgac ccgtgctacc tcgggcggca caacgagatc
    10081 tacgaggagc cgcgcgagct cgtcggcgcc gcgggtgtga agctcaccga gatgccccgg
    10141 cacgcggacc ggtccttctg ctgcggggcg ggtggtgccc gcatgtggat ggaggagaag
    10201 atcggcaagc gggtcaacct cgaacgtgtg gacgaggcgc tcggcaccgg cgcggagaag
    10261 atcgccaccg gctgcccgtt ctgccgcgtg atgttctccg acggcctgac ccagcggcag
    10321 agcgaggaca agggcaccgg cgtcgagatc ctcgacgtct cccagatgct gctcgccgcc
    10381 gtgcaacgtg gtgacgccgc gcgcgacggg gacgccaacg gcaaggtcga cgccctcggc
    10441 gcgggctcgc cgcacaccgc cgacaccact cccaccagcg gaacccaggt caacggaccc
    10501 ggcgacgagt cagcggtcaa cgccgaccct gccggagacg tgtcggtggg tagggccggc
    10561 actggcggcg ccgaaccgaa gggcgccacc tcgagggacg agcccgcggg cgggaactga
    10621 tccggctccg ggcggtttcc tactcgctca gcagggacgg aagccggtca cgacgtagcg
    10681 ggacggcgcg acggcgccga tcgtgaggtg gccgacctgc gggccggcag gcgcacgcga
    10741 accccagtcc atgctgcacg cgtcgacctc cagcccggtg gcggtgggcg tcgtcgtgac
    10801 gtcggcctgg gcgtatcgga cgggatcgga gacgcgcgcc gcgagcgcca tgacggccgc
    10861 tgtgcagtcc ggctgcccca cggacgccgc gaactgagtc ctggcgggct cggccaacag
    10921 gttgtcgcat atggcaccgg gactacccag ggcgatccgg ttgagcagca gcggcagcac
    10981 gctctgcgga cgtggtgcac cctgatctgc tttgctctcc tgctgcaaca tcccgtacga
    11041 cgccgctgcc tcccgcgcgt cgcgcacgtt cgcccaggcg aatgccagac ccgagaccag
    11101 gcacagcacc gcgcccacgg ccgcgagccg ccaccacggc agctgggcga ggcctgcgag
    11161 caggacaccg atgctcgccg ccagtgccca tgcccatgga ctcgtgctca tcatcaaccc
    11221 gacgacgacc accaggcccg cgatgtgcag gtcccaaccc cgcagcaacc cgtcgagccg
    11281 caggatccgc aggacgacga gcgctccgag gccgaccagc cacgggatcc acgacaagcc
    11341 ggagaacggc cacagcagga gcgcggccgc tgtgatcgcg agccccacag cgcctcgtgg
    11401 ggagcgggtg agccggcgcc agtcgaaccg ggggcgggcc ggcggcgcgg gtggcggcgt
    11461 cggcagcggg tcggggacgg ctggtggggg aacctgcggc cgcgcagcgg gcagccacgg
    11521 gggcggggtg ggtcgtggcg gttccatcgc cgtccgaatc tactgctcgg acggtccgtg
    11581 tcccagtagt agctgtgagt cagcccctgc ggccggcccg gcgccactcc tgcggtgccg
    11641 agccgttgct gcgccggaat cgcttgatga agtagctggt gtcgcgataa ccgaccctgg
    11701 cgccgatcgc ctcgaccgtg acgtccgtct cggccaggag ccgccgcgcc tccgtcatgc
    11761 gccgctcggt gatccattgc tggacggtgc gtccggtctt gcgtccgacg atggtcgtga
    11821 ggtggcctgg ggtgaggccc acggcactcg cgacgtcctt cagcgagatg gggcggtggt
    11881 agctcgcctc gatgacgtcg aaaacggcgg cgagcatcgg ctcgtcccgc agggtgaggt
    11941 cgctcgccac gtccgaggcc agccgggaga cggcgacgag caggagcgtg aggtgcgcca
    12001 gtacggcctc gttgtagccg tcccgccgct cgtgcagctc gcgttccagc gcggcgaatc
    12061 gctcggagat gccggcccgg tcatcgaccg ggacgtggag ccgctgagcg ccacccgcca
    12121 ctccgcgcac gaacgggaag agcaacgggt gcgcgcgcca cgacaggaac gatcccggaa
    12181 ggctcggctc gatgatgtcc ggcgggaagg cgaccgccca cgcctcgggc gcttccgcgc
    12241 cgaccacccc gtcgggtgtc acgacttctc cgggtgccac gacgaacacg tccccggtgc
    12301 cgagcgccca ctcccggtcg tccacccgca tggagctgcc gccgcgctcc acgtaaagga
    12361 gtacgaggaa gtcgtgtgcg tgcggatgca gagatccaaa cccgtcggcg tgacgccctc
    12421 cgcccgacca caggacacgg accggcggca cgccggggag ccggtcgtag gtgcggacgg
    12481 gaatcgcccg gccgccgcga tcgagcgtgc cgacggtggg aagaaccgag gatcgtccta
    12541 ctgctcccac gaatccatca ttggcaccag ccgacgtgcg atcgagactc aaggaggaca
    12601 ccggactatc gtcgcattgc tcagaggagt gccaccatga ccgagcaggt tccgcagcac
    12661 acggggcacg ggcacggcta cctccccgcg atggggcacg atcggctgct gcgcttctac
    12721 gatccgttca cccggctgct cggcgtgcgc tccctgcacc gcacgctggc cgaccaggca
    12781 gacatcgctc ccggtcatcg ggtgctcgag atcgggtgcg gtaccggcaa cctcgcgctg
    12841 ctcgtcgccc accggtaccg cggagcgacc gtggtgggcc tcgaccccga cccactcgcc
    12901 ctcgcccgcg cccggcgcaa ggccacgcgc gatggcctcc ccgtgcagtg ggacctcgga
    12961 accgcgggag acctgcccta cccggacgcg agcgtcgacc gggttctgtc cgcgctgatg
    13021 ttccaccacc tcgacgaggc cgagaagcac cgggcgctga gcgagatcaa gcgcgtgctg
    13081 agaccgggcg gccggctgca cctcgtcgac ttcgggggca accaccgcgg cctcctggcc
    13141 cggctcacgc gccgcaaccc gcggctgcag gacaacgccg gggaccggat ccccgcccgg
    13201 atgcgcgagg cgggtctcac cgacgtcggc gagctcggcc ggggccggct cgacgtcacg
    13261 ttctaccgcg ccaccgcgtg agcgacgcgc accgcgtggc aggctcgttc gcggcgaccc
    13321 attgggcgac ctccgcaccg tcgcgtctgg ggcaccgctg ccgagcaatc tcctgccgct
    13381 tgtgctcgcg gtgctgggcg ggctggccct cgtcgtgacc gccacgctgg cgacgagcgt
    13441 cgtcacgccc tcgccggaac gtgcacagat caccacgttg ccgctgatgt tcgtgatgct
    13501 cggcgtcggc gtcgtgggag cgatcgcgcc cggccagggc tggtggtccg tcctgatggt
    13561 cgtgccggga gcagcggtcg gcgacctggc ccagctcgcc atgacgggcg gcagctggtc
    13621 ggcggtgaca gcggggctgc ccgcgatcct gccgccgctc gtggcgctcg aggtctggcc
    13681 ggtcgtgttc tgggtactcg ccgcgcgcgg attccgctgg gacccgcggc attgaccacc
    13741 ccggacgggc gagctcagcg ccgggtgtcg gcccggtgga agttcaggtg ggcccgcgac
    13801 ggcgtcggcc cccgctgccc ctggtagcgg gagccgacgc cgtggctgcc gtagggccgc
    13861 tcggcggggc tcgacagtcg gaacaggcac agctggccga tcttcatgcc cggccacagc
    13921 gtgatcggca ggttcgcgac gttcgacagc tcgagcgtga tgtggccggt gaaaccgggg
    13981 tcgatgaagc cggccgtgga gtgcgtgagc aggcccagcc ggcccaggct cgacttgcct
    14041 tcgagccgcc cggccagatc gtcgggcaac gacaccgact cgaacgtgga gccgagcacg
    14101 aactcgccgg ggtgcagcac gaacgggtcg tcgccgtcgg gctcgacggc cgaggtcagc
    14161 tcgtcctgct gctgcgcagg gtcgatgtgg gtgtagcggg agttctggaa cacgcggaag
    14221 aaacggtcca gccgcacgtc gatgctcgac ggctggagca tcgcagggtc ccacggttcg
    14281 aggacgagac gcccggcttc gagctcctta cgcaggtcac cgtccgacag cagcaccggc
    14341 ccagcgtatc ggcgcgccgt cagacctccg tattggtggc cgagtcgttc gcggagaacg
    14401 ggaggttcgg ggtgagcagc ggtgatgggc acaacccgaa catccaggcg acggcctgct
    14461 cgacccgcac ggcctcgagg ccgtcggacg gtgcgggcgc cagcttcggt gtggccacga
    14521 ggacctgccg gaccgtggtc atgacggccg ggctgaggat gctgcggatc gagtcgccgg
    14581 gctccgggat gtgcggaccg tcgacgagta cggccggtgt gccgaggccg ggagcgtcga
    14641 tgaccagctc cgggtcgtcc gagaccagca cggcgctcgc cgcgatgagc cagaccagct
    14701 ccgcgagcgg caggttgcgt acgacgatgg cccgagggtg ctggagaagc gggtaggcgg
    14761 cgccgtggct cgcgagctcg ccgtagacga cgatctcgat gtcgggttcg cggtcgagca
    14821 ggtcggggag accggcgagg acaccgagcg agtcggggcg gtccagcccg acgagcacca
    14881 cctgtgggcc gccggctcgc acccggcgga tgagccgcgc gaaccggagg tcggcaggct
    14941 gggggttggc ggccatggtg tcgcccaccg agatggcgtt cggcccgacc cgactgccca
    15001 gcgtcgcgtc cccggtggtg gtgaggaaca gcgacgcgag ctgaccgatc acgcgccggt
    15061 tggcctcctg ggggaacggg cagagcaggt cgtccgtcgc cacgccggcc tgcaggtgca
    15121 cgaccggaac ctgccgccag aaggcgacct gggcggccac cacggctgtc atcccgccgc
    15181 cgtaaaccat gacagcggac gggtcgaggt cgacgagcag ctcgtcgagc cgcgtcatca
    15241 gcagggacgc gatcgcggcc ggctgcgggc cgggtggctc ccgcagcagc tgcgtgacgt
    15301 cggcaggcac cccgagcgac tcgaaggcct cgtggacggt catcggatcg ggtccggtgg
    15361 ccacggtgag agcacggatg cggtcggcgt tggcgaacgc ggtggcaacg ggcgccaggc
    15421 gggcgacttc ggggcggctg ccggcgatga ggagcacgtc gtggtcgtgg tcctgatcgg
    15481 ggagcgaacc catgggctgg tcgagctcag tcatgctcgg gtccttcgcg gccgggggag
    15541 ctggccttac ggggctccag cagtgtgaac tggtggtcta ccggccgtcg agcccctatc
    15601 tcacaaagcg ggtcattaga gtgggaacca cccaagtgga gtacggaact ggccttgatg
    15661 gttacgcagg gtgagctgct agagtgtgcg ttacacactg cggatgtagt tcaatggtag
    15721 aacatcagct tcccaagctg aatacgcggg ttcgattccc gtcatccgct cgcaccgcga
    15781 ggcccaggta cgaggaatct cctccgatcc tgggccttcg tcgtatcgag gatcgacggc
    15841 cttcgtgcga tccacgcacg gtcagctcac cgggaccagg tggccggctg gaccgtcttc
    15901 ctcgccctcg gggctcttcc gacgcgtgcg acggtgctcg tccaggagtc cgctttgccg
    15961 gtccgcgatc agccggtcgg cctgactggt cgcgtgctga tcgatcaacg cccgcatgtt
    16021 gtcgtgcccc attcgcgaca tcaggtcctt ggtgctcgcg ccggtctgcg cggccagagg
    16081 ttcccgtgtg ccgcagctcg tggcagcgga ggccgggcgg ccgagcttct tcaccgtctc
    16141 cacccgcttg gtgaggttga tgaagtgcgc ccgacgcaag gcacgccctt cggaccggtg
    16201 aacacgagcg catcgggagc cgccgtgatc gccgcggcaa ccgacgtacc gggcgcggcc
    16261 tagcctcagc tgcatgcctt cggtcctcgt tctcggcttc ggtcctcagc tcgtgcccgg
    16321 tgccgacgga gacggcgtac tcgcggtcct cgaccgcgag ctggctcgct tcggcgagca
    16381 cgacatcgat gcgtccgtga cgctgatcgc gcccgaccgg acggcggaga ccaccgtggt
    16441 cgcagcgtcg gccgcgcaac cgtgggatgt cgtcgtcgtc ggaggcggga tccgcaagcc
    16501 ggagccggtg ctgacactct tcgagaagct cgtgaacctg atccggcagc atgcaccgca
    16561 ggctgcgatc ttgttcaaca cgagccctgc cgacaccgtc gaggcagcgc tgcgctggct
    16621 ctgacgccct cgtccgatcc tctcgcagga cgcacccaag ctagccgaca acaggttctt
    16681 gcgcgcccag gtggcacaag ggctcaggct gctgcgattc aggctcgtga gtcgcgacag
    16741 cggtcccaag aggggccggc tacgtcggac tcctgtgaat ggctgaattc gcgacggatt
    16801 gtgcccttcg actgcctgga tcaacgatcg cttcggagtg tgtccgcgtg ccacgtgtca
    16861 tccgctccac caggaacaat gagtggattg cgtcgctcac tcaggaagta gcggaaccgt
    16921 gactcccggg tcgcggccga gaaggacggc acgtgtgatg gctgcggatc cgaaacggtc
    16981 atgcagggtg tcgagcacgg cgtcgagggc ggcgcccgca tcctcatcga agggcagcac
    17041 gagctggacg gcgtcatcgt cgtcgaggtt gccgaccgcc accccgacaa gggtgaggcc
    17101 ccgttcctcg atcaacggca tggccctcgc cacgagcacc cggacggtgg tgaggatcgc
    17161 ttcggtggcg caggtcggct ggggcaacgt atgggaacgg gtgaggcgcg agtagtcggc
    17221 gaggcgcaag cggagcacga ccgtacggcc gacccgtcct gcggtccgca gccgccgggc
    17281 gatgcggtcg acgagcgcga cggccacggc gtcgatggct gccggggtgg tgggcgcgcg
    17341 acgccccagg gcgcgttgcg cgccgatcga gcgccgcctc cggcccacct gcacgggtcg
    17401 ggggtcgagg ttgtgggcga gcgcgtgcag gtgccggccg agcgcccggc ccagaatcga
    17461 gaccaggatg ttctccggga gctcggcgac ctcgccgacc gtcgagatcc cgtgctcgcg
    17521 cagcttggcc gccgtcacct tgccgacccc ccagagccgc tcgaccgcga gcggatggag
    17581 gaagccgagc tcgtcctcgg gcggcaccac gagcaggccg tcgggcttgg ccacaccgct
    17641 cgccaccttg gcaaggaact tcgtcctcgc caccccgacg gtgatgggca gaccgacccg
    17701 ctcgagcacg tcggcccgca ggcgcgccgc gatcccactc ggcgggccgg cgatccgccc
    17761 gaggcccccg acgtcgagga acgcctcgtc gatcgacatc ccttccacca gaggcgtggt
    17821 gttccggaac acctcgaaca cggccttgct cgcagcggag taggccgaca tccggggcgg
    17881 cacgacgatc gcgtgcgggc acagcgaccg agcctgtgtg ccgcccatgg cggtggcgac
    17941 cccatacgcc ttcgcctcgt agctggcggc gaggacgacg cccccgccga cgatcacggg
    18001 acgcccacgg agccgggggt cgtcgcgctg ctcgaccgac gcgtagaacg cgtccagatc
    18061 ggcgtgcagg atcgtcccct cgcgcgacac gaacatatgt tcgcatcgtt gggcggtgga
    18121 cgcgagcatc gtggacgctg cgcgggcggt ggattccacg accgtgagga tgccgaaggg
    18181 tcgcaggacg gacgcgatcg gtcaggactc cagcacctga gccatcaaca tggtcgcggg
    18241 gccgaacccc gcacgagcgt agaacggcac ggagcgctca ctcggcgaca gcacgagcct
    18301 gacgcaaccg atctcccgcg cgtgacccac gagctggtcg agcagcgccg agccgatgcc
    18361 ccggttgcgg tggcgctcga ggacgaacgc gttggcgaga tatccccacc gcgtcggggc
    18421 acgctccggg ctcggcatcc gctcgaagac caccaggtgc atcatcccga ccagccgccc
    18481 cccggcctcg gcgaggaagg agacccgagg gcccgccact cggatcgtca cgccgtgggt
    18541 catgacctca tcgtgccgtc gtgcgggcct cgcgccagag ggagatgtcc acgtagtggt
    18601 tgtcgaagcg ctcctgcgac tccccgatct cccggggctc ggcatcaccg cgatggaccc
    18661 ggtcgagcag gcgatagtag tcgaaccggt ccatgccggg ggtgaagacg acgagcacgt
    18721 ccgcgtcgac gccggctgct gcaccgaagg cgtgcggcac gtgcggcggg acgagcagga
    18781 cgtcgccctg gtccaggatg tgcaaccggt ccccggcgag cacctgcagg ctgccgccga
    18841 gtacgacgaa cagctcggac gcgcgggtgt ggaagtgcgg cggagcgccg tcggcgccgg
    18901 ccctgaaggt cgacctgttg caggtgatcg ctccacccgt gctgacggag tcggcgagca
    18961 gggcgatcgt gccgccgggg tcaccggtga gggtctcggc gtcggacgcc cggacgagca
    19021 gtggtgtcgt ggtcgtgttc atgtcgtcca cgatggccac gggcggagcc gcgaacaacg
    19081 acgaaaacgg ccacgaccga taacctgtgc gttatggaga tgcgggagat cgagatcttc
    19141 ctcgcgctcg ccgacgaact ccacttcggg cgcgccgccg agcggctgcg cgtgtcccag
    19201 gcccgtgtca gccagacgat caagaagctg gagcggcagg tcggcgcccc gctcttcgac
    19261 cggaccagcc gccgcgtcgc actcaccccg atcggcgcca ggctgcgcga cgacctcgcg
    19321 cccgcccacc ggcggatcat cgacggcgtg accagggcga ccgacgccgg ccgcggacag
    19381 ggcggctcgc tgcgtctcgc gttcgaggca ccgggggtcg ccgatctcat cgccgaggtg
    19441 ctcgacgcct atcgcttctg ctcgcccgac agcgacgtgc aggtccggga ggccgatttc
    19501 cgcgacccct tcggtctgct ccgcgggggt gaggtggacg cgctggtcac actgctgccc
    19561 gtcgccgaac cggacctgac cacgggaccg gtcgtcctca ccgaaccggt ggtgctggcc
    19621 gtgccggtac ggcacccgtt cgcccggctc gactccgtga cgctcgacga cctcgcgcgc
    19681 gacaccgtgt tccgcgccgc tcggcccgcg ccgccctact gggtcgaacc accacagccg
    19741 tgggcgaccc cgggcgggca cccggtgcag cgcggacgcc cgctcacgac gtttcaggag
    19801 ctgctcgcgg tgatcgcggg tggcgagggc atctgtcccc tcgccgcaca cgccacgcag
    19861 tacttcgcgc acccgaagat cgcgttcgtg ccgttccggg acgcgccgca cgtcgagtgg
    19921 gccctcgtgt ggcggcgcgc aggcgagaca gggcgtgtgc gcgagctcgc gcggtgcatt
    19981 cggacgggag ccggccggac cgtcgctacc cgcgcggagc ggcgagaacg cctgtgatca
    20041 cgagcgttcc gcgtcgaacg ccttgcgcac gcggacgggg gcccggtcgc gccaggcgtc
    20101 ctcaatcagc tcggtcagct gctggtgcga caccttctcc aggtcgacga ggatcgcgcc
    20161 gtacccgtcg tagtgggggg tcgtgtagaa ggccgggtcg ccggaagcca gcagcgcctc
    20221 cttctcgtcg atctgacaca tcaggacgag cccgccttcg gcctcggtgc gcagccgcgc
    20281 gaagctcttg ccctgcaccg tgagggaagg ggtccggtag gaggtcgact gctcgacctc
    20341 gggcagcgca accgcaatcg ccacgacgtc gtcccaggtc ggcatgcgga catcctggcc
    20401 tgcacggtgc ccgacgtcgt ggacgaatcg gaggtcagcg cacccgggga ggtcgaagcg
    20461 gtggggcctt ggcgagcacc acggtgccgg cgagtgctgc cgccaggccc actgcgagca
    20521 cccacgccca gccggggcgt accgggtcgt ccagcagttg gatgccgacc atgcccggta
    20581 cgagcacctc ggtcaccgtg aagaccgcgg tgaccggacc gacgtcgccg cgcgacagcg
    20641 cagcggtgta gccgaccatc ccgaccagcc agaacccgac caccaagtac gtggccggtt
    20701 ggccgagcag cggcaccagg tcgaatcgtt ccccggtctg gacgtgcgcg gcgcgcaccg
    20761 acagcgcagt gccaccgaac cccagtccgg ccaccagggc gagcggccag gagtgtttgc
    20821 cctgccgcag caccagcacg gcgacgccca gtagaagcag cgagaccagc aggatgacgt
    20881 cgacgccacg ggtgcggacc ggtgggtggt ggctccccgc gctggcagcc accatgacga
    20941 gaccggtcag gcacaccccg acggccaccc gggtcctgac ggggagccgc actccgagca
    21001 tccgggcgtt gaagactgcg gtgagcgcga tcgtgccccc gatgatggcc tgcacggcga
    21061 agacaggcag tacccgcagt gccagcacgg cgcaccccca cgcgaggaag tcaaccgcga
    21121 cgccggcgat gtaccggggc tggatcgcgg cgggccgcgt ccgcgtggcc cggcgggcac
    21181 cttcggcctg caggagagcc ccgaaggtgt tcaggaccat cgcgaccgtc gcgagcagga
    21241 tcccgaccag cacgttgtca gcatgccgtt ccgtgaccgc cgacgctcgc ccggcccgcc
    21301 gggagcagcc ggttcacacg gccgagaaag ctggtccccg ggataacggg agccgcatca
    21361 ccggcatcct ccggccgagc ggcgagtccg tccagccggc cgtgacgccg cccatccgct
    21421 cgtagaaccc tgtggcatac ggctccgcct cccactcgag gacggtgctg cccgctgccg
    21481 tcgctcgggc cacggcgtgc tcgaacagca gccgacccga cccccgtccg atctcggccg
    21541 gctcgagcca caggtcgtcg agaacggcca gcgcgccacg gtgcagcagc gtgtagaaac
    21601 cgcggacctc acctgcgcgc tccccgacgt tcacgtcgtt cgccgccacc acgtcgggcg
    21661 tgagggcgag cgtgcgcgcg aaacgggcga ggaacgctgc cggatagggc cagtgcccct
    21721 tggaacggac ggcgagcgcc gccaggaaag cggcctcgtc aggacgggcc ggccggatga
    21781 gcatgagacc agtctgcccc gggcgcgatc ggtcagcgac ctccgcgatc ctcgccgctg
    21841 gcgaccgggg tgaagaaccc gctcatcagg ccgccggcaa cggcggaacg agcatcccac
    21901 cgctccgcgc ggtgttcgtg cagtttgcgg cgcggcccct ccgacgcccg cacgaacgcc
    21961 agcaccagca ccgtcccgag caccagcggt gctcccacca ggagcaccag ccagccgaaa
    22021 gcgacgtacg caacacccaa cgcagcgatc tccatggcga tgaccgcccc gcccaaccga
    22081 accacgacga ctttccccct caacccggcg ccgctactgg cgccctacga cgacctatcg
    22141 ccgcagcctg ctcccgcgtg acaggtatga cacagagcga cactgaagaa acagaaagtg
    22201 acggtcgcga gcaatcccga ctggatgaat ttctacggac gacaccctcg ccgggcggac
    22261 ggcgggcggt gggcacggct catacggccg gggacgccga cccctctgct gcgggcacgg
    22321 gctgctcggc cacgggagac cagctaccgg tcatgccacc tggcgaggtg acaccgcctg
    22381 gcggcgtgcc cgctgacggc gtgccgccgg ccgacgggat gccaccagcg gacggggggc
    22441 caccagcgga cggggggccc actgacggcg tgccaccacc cgacggcgtg tcaccgccca
    22501 ggactccacc ggaggcggtg ccgcccgtgg aaggtcccgg ggtccccgta tcggctccgg
    22561 tcgggtcgcc ggcaggtccg ggacttgtgc tggtgacgtc cgacctcggc ggcgtcggag
    22621 tcgtcacggg cggcgggtcg acgggcgttt cgcccagcgc cggatcgact actgcggccg
    22681 tcgtcggcac cgtgccaccg ccaccgcggc caggtttgtt cgtaccgcct ccgggtgcga
    22741 caccaccggc ccgcccagct cccgcaccgc cgggagcgct gttccc
//
LOCUS       DASRSC010000274         4939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4038815,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000274 DASRSC010000000
VERSION     DASRSC010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4038815"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(645..1400)
                     /locus_tag="VFE39_13500"
     CDS             complement(645..1400)
                     /locus_tag="VFE39_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6259659.1"
                     /translation="MSGHSKWATTKHKKAVIDARRGKMFAKLIKNIEVAARTGGGDPD
                     GNPTLYDAIQKAKKSSVPNDNIDRAVKRGSGADAGGADYQTITYEGYGPNGVAVLIEC
                     LTDNRNRAATEVRTAMTRNGGAMADPGSVAYLFTRKGVVIVPKSGDLMEDDVLLAVLD
                     AGAEEVNDLGESFEVVSEATDLIPVRTALQDAGIEYDSADPTFLPSMSVPLDAEAAKK
                     VFRLIDALEDSDEVQNVYANFDVSDEVLEEVNA"
     gene            complement(1615..2262)
                     /gene="pdxT"
                     /locus_tag="VFE39_13505"
     CDS             complement(1615..2262)
                     /gene="pdxT"
                     /locus_tag="VFE39_13505"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET6259660.1"
                     /translation="MGRVVPVIGVLALQGDVREHLAAVTACGDVRGVAVRRPSELDAV
                     DAVVIPGGESTTMSRLLQVFELLEPLRERIAAGMPTYGSCAGMILLAREVLDGRPDQQ
                     QLGGLDVVVRRNAFGRQVDSFETDLRFGGLLGGPVRAVFIRAPWVESAGPGVEVLATV
                     PRLTLTGADAGAAAGRPVAVRQGSVIATAFHPELTGDLRVHGLFCDLVKEAARAA"
     gene            complement(2282..3676)
                     /locus_tag="VFE39_13510"
     CDS             complement(2282..3676)
                     /locus_tag="VFE39_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HET6259661.1"
                     /translation="MPVPGPGYAITARVEVPASASAAGDLTMAVGHVGGVVTAFDVVE
                     AQTDTLVVDISCNALNEAHAEEITAALEALPGVRVRKVSDRTFLLHLGGKLTVESKVS
                     LRNRDDLSRAYTPGVARVCQAIAANPADARRLTIKRNTVAVVTDGSAVLGLGNLGAAA
                     AMPVMEGKAALFKRFAGVDAWPVCLDTQDTEEIIRTVQILAPAYGGINLEDIAAPRCF
                     EIEARLRELLDIPVFHDDQHGTAVVVLGALRNALRVVGKKLSDCKVVVCGVGAAGSAI
                     IRLLRSGAPGAVLAVDIDGIVHPGRTGLDDNLTWIAAHTNESGVSGSLADALVDADVF
                     IGVSAPNLFGAEELATMAPDAIVFALANPDPEIDPAIATAHAAVVATGRSDYPNQINN
                     VLAFPGVFRGLLDAQARDITDAMLLAASAAIADVVTEPNPSFIVPSVFDAAVAPAVAE
                     AVRTATAQRDVAPV"
     gene            3843..>4939
                     /locus_tag="VFE39_13515"
     CDS             3843..>4939
                     /locus_tag="VFE39_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HET6259662.1"
                     /translation="MAVRADAGQRAGSRSSVPTVSDPTRVRNVVLVGPSGSGKTTLVE
                     ALLAHAAAIPRAGAVVEGTTVCDHDPAAVRQQRSVALSVAPLLHEDTKINMIDTPGYG
                     DFVGELRAGLRAADAALFVVPANEGREGAIDPATVAQWEECAAVGMPRAVVVARCDHA
                     RADLEATIAACQDAFGSGVAPLYLPIREGERLTGLYGLLTQTPAGQAPPGAEDGRGEL
                     IEGIIEQSEDESLMDRYLGGEELDVTTLIDDLETAVARGTFHPVIPVCASSRIGLDGL
                     LEVLVGGFPSPLERPLPPVTGVDGTAHPSLTADPDGPLAAEVVRTSADAYVGRVSLVR
                     VFSGTLRPESTVHVSGHHVFIPAQRREEPDGG"
BASE COUNT          740 a         1748 c         1733 g          718 t
ORIGIN      
        1 gtaaacactc cggcctgacc gggtgccacg ctggatgatc tttcctgcct cgtgccggag
       61 attcttccgc agatcttcgc cagtgtgtac gtggcttcgg ctgaccggtt accgtatcga
      121 cctgtcggag cagaccagca cccttaaaaa tgtgatccac cgagaccgca gtatgcggga
      181 aaccggctga gcggtccact cgccctttct ccgggacggt accgcgccgc ttcagatcac
      241 cactatcgag cggatggact cgttccgtgg gcgcggaccg acaagctcac gcactccgcg
      301 tgcgggaaag tcattcagcg cgacttacag aaaagccgcg acgagcgaga gcaccgtcac
      361 gacgtaggac cagccgaaaa ttcgaacgtg cacatcccct gttcctgcac cacgacgcgc
      421 cggcgcccgc cccaccggcg tcgccctccg atgatctacc cccgggagcg cgcccggttt
      481 gggcagacat ccatagtgcc caagaagcta gggagggtgg gatgatcgcc gaacacgtca
      541 cgccggggca gcgctcacgc agtcggggtg cgcagcgggg gtcagccgac ctccgccatc
      601 acctcggcgc tgacgtggaa tgcgacagtg gcgtacgcca acttctacgc gttgacctct
      661 tcgagcacct cgtcggacac gtcgaagttc gcgtagacat tctgcacctc gtcgctgtcc
      721 tcgagcgcgt cgatcagccg gaagaccttc ttggcggcct cggcgtcgag cggcaccgac
      781 atggacggca ggaaggtggg gtcggccgag tcgtactcga tgcccgcatc ctgcagcgcc
      841 gtgcgcaccg ggatgaggtc ggtggcctcc gagacgacct cgaagctctc gccgaggtcg
      901 ttgacctcct cggcaccggc gtcgagcacg gcgagcagca cgtcgtcctc catgaggtcg
      961 ccggacttcg ggacgatcac cacacccttc cgcgtgaaga ggtaggccac cgagccgggg
     1021 tcggccatgg ccccgccatt gcgggtcatg gcagtgcgga cctcggtggc ggcgcggttg
     1081 cggttgtcgg tgaggcactc gatgagcacg gcgaccccgt tggggccgta gccctcgtag
     1141 gtgatcgtct ggtagtcggc gccgccggcg tcggcacccg aaccgcgctt gaccgcgcgg
     1201 tcgatgttgt cgttgggcac cgaactcttc ttggccttct ggatggcgtc gtagagcgtc
     1261 gggttgccgt cggggtcacc gccgcccgta cgcgcggcca cctcgatgtt cttgatcagc
     1321 ttggcgaaca tcttgccgcg gcgggcgtcg atgacggcct tcttgtgctt ggtcgtcgcc
     1381 cacttggagt ggccgctcat cgagtttcgc cgtcctttcc cagctcctgc agaacccgcc
     1441 gggatcccgg acgtccgcct ccggccacct gccgcgcgga ccggatgaca ccgacctctg
     1501 cctgatcggg acgattctac cgacccgctc ggacgacggg cggccgggaa ggggcttcgg
     1561 cctgacggcc ccgtgcgcgg acgcgtcagc cgcggggcgg cgcccggccg cggctcaggc
     1621 cgcgcgggcg gcctccttca ccaggtcaca gaacagcccg tgcacccgga gatcaccggt
     1681 gagctccggg tggaacgcgg tggcgatcac cgatccctgc cgcactgcga ccggccggcc
     1741 tgcagcggcg cccgcgtcag cgccggtgag cgtcagccgc gggacggtgg cgagcacctc
     1801 cacgccagga ccggcggact ccacccaggg cgccctgatg aacaccgcac ggaccgggcc
     1861 gcccagcagt ccgccgaacc gcaggtcggt ctcgaacgag tcgacctgcc gaccgaacgc
     1921 attgcgccgc accacaacgt cgagcccgcc gagctgctgc tggtcggggc gaccgtcgag
     1981 cacctcgcgg gcgagcagga tcattccggc gcacgacccg taggtcggca tcccggcggc
     2041 gatacgctcg cgcagcggtt cgagcagctc gaagacctgc aggagcctgc tcatcgtggt
     2101 cgactcgccg ccggggatca cgaccgcgtc gaccgcgtcg agctcggacg ggcggcgcac
     2161 cgcaacgcca cgcacgtcgc cgcaggccgt cacggcggcc aggtgctcgc ggacgtcgcc
     2221 ctgcagggcc agcacaccga tcacgggaac cacgcgacca atgtagtcgg cgccgcagcc
     2281 gtcagacggg ggccacgtcc cgttgcgcgg tggcagtacg gaccgcctcg gccaccgcgg
     2341 gggcgaccgc ggcatcgaag acgctcggca cgatgaacga cgggttcggc tcggtgacca
     2401 cgtcggcgat cgcggccgat gcggcgagca gcatcgcgtc ggtgatgtcg cgggcctggg
     2461 cgtccagcag gccgcggaac accccgggga acgcgagcac gttgttgatc tggttcgggt
     2521 agtccgagcg gccggtggcc accaccgccg cgtgggcagt cgcgatggcc gggtcgatct
     2581 cggggtcggg attggccagg gcgaagacga tggcgtcggg agccatcgtg gccagctcct
     2641 cggcaccgaa caggttgggc gccgacaccc cgatgaacac gtcggcgtcg acgagcgcgt
     2701 cggcgagcga gccggacacc ccagactcgt tcgtgtgggc ggcgatccag gtcaggttgt
     2761 cgtcgagtcc ggtgcggccg gggtggacga tcccatcgat gtcgaccgcc aggacggcac
     2821 ctggcgcgcc ggagcgcagc agccggatga tcgcggagcc agccgcaccg accccgcaca
     2881 ccacgacctt gcaatccgac aatttcttgc cgaccacgcg cagcgcgttg cgcagcgccc
     2941 caagcaccac caccgccgtg ccgtgctggt cgtcgtggaa gaccgggatg tcgagcagct
     3001 cacgcagccg ggcctcgatc tcgaaacagc gcggcgccgc aatgtcctcg aggttgatgc
     3061 cgccgtaggc cggcgccagg atctggacgg tccggatgat ctcctcggtg tcctgggtgt
     3121 ccaggcagac cggccaggcg tccaccccgg cgaaccgctt gaacagcgcc gccttgccct
     3181 ccatcaccgg catcgcggcg gcggcgccca ggttgcccag gccgagcacg gcggagccgt
     3241 cggtgacgac agcgacggtg ttgcgtttga tcgtgagccg gcgcgcgtcg gcggggttgg
     3301 ccgcgatggc ctggcagacc cgcgcgacac cgggcgtgta ggcacgggag aggtcgtcgc
     3361 gattgcgcag gctgaccttg gactcgaccg tcagcttccc gccgaggtgc agcaggaacg
     3421 tccgatcgga taccttgcgc acccgcacgc cgggcagcgc ctccagcgcc gcggtgatct
     3481 cctccgcgtg cgcctcgttg agtgcgttgc agctgatgtc gacgacgagc gtgtcggtct
     3541 gcgcctccac cacgtcgaac gcggtgacga cgccgccgac gtggccgacg gccatcgtca
     3601 ggtcgcccgc cgcgctggcg gatgcaggca cctcgacccg cgcggtaatg gcatatccgg
     3661 gaccgggtac aggcacgcca agacactacc ttcggcgctc gcgccgccgc gttgcgccgg
     3721 gcctccacgg catgtgatat gcctccggcc ggccggaggg gttgtgacca ccggacctcg
     3781 ggtgtgagat gacgcacatg tgacgttcgg acgtcgacgc agtggtgcgg aggtggtcac
     3841 ccatggccgt cagagcagat gccgggcagc gtgcggggag ccgatcatcg gttccgaccg
     3901 tcagcgatcc gacccgggtg cggaacgtgg tcctcgtcgg gccgtcgggg tcggggaaga
     3961 cgacgctggt ggaggcgctg ctcgcgcacg ccgcggcgat cccgcgcgcc ggcgcggtgg
     4021 tggagggcac cacggtgtgc gaccacgacc cggcggccgt ccggcagcag cgttcggtcg
     4081 cgctgtccgt agccccgctg ctgcacgagg acacgaagat caacatgatc gacacccccg
     4141 gctacgggga cttcgtcggc gagctgcgtg cgggactgcg cgccgccgac gccgcactgt
     4201 tcgtcgtccc ggccaacgaa ggacgcgagg gcgcgatcga tcccgccacc gtcgcgcagt
     4261 gggaggagtg cgccgccgtc ggtatgccgc gcgccgtggt ggtcgcccgc tgcgaccacg
     4321 cccgtgccga cctcgaggcc accatcgccg cctgccagga cgccttcggc agcggtgtcg
     4381 cgccgctcta cctgcccatc cgggagggcg agcggctcac cgggctgtac ggcctgctca
     4441 cgcagacacc tgccggacag gccccgcccg gcgccgagga cggtcgcggt gagctgatcg
     4501 agggcatcat cgagcagtcc gaggacgagt cgctcatgga ccgctacctc ggcggcgagg
     4561 agctcgacgt caccaccctc atcgacgacc tggagacggc cgtcgcccgc ggcacgttcc
     4621 acccggtgat cccggtgtgc gcgagcagcc ggatcgggct cgacgggctg ctcgaggtgc
     4681 tcgtcggcgg gttcccgtcc ccgctggagc ggccgctgcc gccggtcacc ggagtggacg
     4741 gcaccgcgca cccgtcgctc accgccgacc cggacggccc gctcgccgcc gaggtggtgc
     4801 gcacctcggc cgacgcctac gtggggcgcg tctcgctggt gcgggtgttc tccgggacac
     4861 tgcgcccgga gtcgaccgtc cacgtcagcg ggcaccacgt gttcatcccg gcccagcggc
     4921 gtgaggagcc cgacggtgg
//
LOCUS       DASRSC010000275         6241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4039132,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000275 DASRSC010000000
VERSION     DASRSC010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4039132"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VFE39_13520"
     CDS             complement(<1..204)
                     /locus_tag="VFE39_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259663.1"
                     /translation="MRLSTAAPKIWARGAVTLGIALAAALTVACAPVQQAAGPGAAPA
                     TDVFGTPADPAAVQRGGTLTMALS"
     gene            410..832
                     /locus_tag="VFE39_13525"
     CDS             410..832
                     /locus_tag="VFE39_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HET6259664.1"
                     /translation="MPALAPAEQRARLAAASVARLATVRADGTPRLVPITFALVEGLV
                     CSVVDEVKPKRSPRLARLDDVRRDPRVALVVDHYEDDWSALWWVRVDGTAEIHEAGPL
                     RERALAELRAKYPPYAAAPPAGALLVITPSRWSGWTAA"
     gene            887..1321
                     /locus_tag="VFE39_13530"
     CDS             887..1321
                     /locus_tag="VFE39_13530"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6259665.1"
                     /translation="MRSLSITAIGELLGIPLDERRQLRRWADTARRSRQPRPRPASRP
                     RRFDPARRPNPHLAFGHGQHHCLGAALARLEIGVALRALAVALPGLRPRDPIGDIPWI
                     HGLVDSGPAAIRIARPVTVALPRERTAEITTVCLRVRAGVAR"
     gene            complement(1323..2483)
                     /gene="hppD"
                     /locus_tag="VFE39_13535"
     CDS             complement(1323..2483)
                     /gene="hppD"
                     /locus_tag="VFE39_13535"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HET6259666.1"
                     /translation="MTTGGITDALIDPLIGEVAHDDSTDAFPVLGLDHVRFAVGNARQ
                     AAHWYSSAFGMRVVAYRGPETGHRDLAEYVLTSGSARFVLAGEVHAGTWVGARVAERG
                     DGVADLAITVPDVDAAVAHARAAGAMVLTEPHTVSDEHGELRRAAIATYGDVRHTLVD
                     RSRYHGPFAPGFVAREPLFPAAGKRCFQAVDHAVGNVELGRMDHWVSFYNRVMGFTNM
                     AEFVGEDIATEYSALMSKVVASGNHKVKFPLNEPAAGRRRSQIDEFLEFHGGPGVQHI
                     ALATGDIVASVRAMRAMGVEFLATPDSYYDTLTDWVGDTRVPVETLRELGILADRDED
                     GYLLQIFTRPVQDRPTVFFELIERHGSLGFGKGNFKALFEAIEREQEKRGNL"
     gene            2577..3092
                     /locus_tag="VFE39_13540"
     CDS             2577..3092
                     /locus_tag="VFE39_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET6259667.1"
                     /translation="MTTPASSPDALDVELIGLLSEHPRAGALELSRLARVARATVTAR
                     LERLERAGVITGYGPDVDLRAAGFPVQAFVSLEIAQGAIADVRAELLAIPAVLSAHAT
                     TGTADVLCRLAAASHEDLHATLLQIDRSPAVLRSTSVVVLSEVVASRYLPLLRGTGRP
                     RPSRAPAYREC"
     gene            complement(3404..4168)
                     /locus_tag="VFE39_13545"
     CDS             complement(3404..4168)
                     /locus_tag="VFE39_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6259668.1"
                     /translation="MAERRCVEVPGGRIEYEDVPGDPTVAPLLFLHEGLGSLGLWRTF
                     HHRIAAATGRRAVAYSRLGHGFSDLPTQKRTPRFMHDEAATVVPALCAALGLDRPVLV
                     GHSDGASIALLYAARSSDVTGLVVLAPHEFVEEIGLGGIEQARRAYTEGDLRARMARH
                     HRDPDVTFWNWNDVWLDDAFRDWDIRFELPAITCPVLAVQGTADEYGTLAHVEAVRDA
                     ATGPVELLVLDCGHSPHLQRPEDTEEAVTAFVSKLP"
     gene            complement(4224..4577)
                     /locus_tag="VFE39_13550"
     CDS             complement(4224..4577)
                     /locus_tag="VFE39_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET6259669.1"
                     /translation="MTDLLARLRALCLALPEVTEKVSHGEPTWFVRKMFVTYADQHHD
                     DRVAFWCAAQPGTQEELVAAAPTRFFRPPYVGGRGWVGVCLDLDDVDWDEIAELVTDA
                     YRVIAPKKLLARLNR"
     gene            complement(4574..4972)
                     /locus_tag="VFE39_13555"
     CDS             complement(4574..4972)
                     /locus_tag="VFE39_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6259670.1"
                     /translation="MSIHPTLLYNDSKAAIAFLTGALGLVEEQVHTADDGTVGHAELS
                     FGDGVVMLGTRSDPPGPFDTGRAVIYLVLDGADAVDAHHDRAVAAGAEVVQGLVDQPY
                     GSREYAVLDPEGNVWSVGSYRPQVKGVGDA"
     gene            complement(5025..5846)
                     /locus_tag="VFE39_13560"
     CDS             complement(5025..5846)
                     /locus_tag="VFE39_13560"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6259671.1"
                     /translation="MEAPVGEYVRGRPHPALAEHVLGYSGYRERSAAPVRRRQAPTGS
                     CTLILSLGPPIRLHGPAGPTVPVSFLAGMHDAAVITEFTGVQHGVQVDLTPLGVFTLL
                     GRPMPELTNRVPHVDELEVPELTALPARLAEEPGWPERFDRVDDALLRRLDASRARPD
                     PEVAWAWDRLVRTAGAIGIQQLADGTGWSRRHLLNRFRMQVGLAPKPAARVLRFQRAA
                     ELLLPPGPDGGSRARAARSVADVAATCGYADHAHLVREFRTLAGCTPTEYLAERS"
     gene            5894..>6241
                     /locus_tag="VFE39_13565"
     CDS             5894..>6241
                     /locus_tag="VFE39_13565"
                     /inference="COORDINATES: protein motif:HMM:NF024586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HET6259672.1"
                     /translation="MDRPDDLLAELVGMLPVGIFAVDGDGCVLLWNAAAERLTGWSGS
                     DVIGQRVMGPDPTRRTFDAPTAARILDELRAGRTWSGRVPATPVSGRTLYFRAAPGPG
                     STIVGVLQDVDDTE"
BASE COUNT          866 a         2259 c         2254 g          862 t
ORIGIN      
        1 cgacagcgcc atggtgagcg tgccgccccg ctgcacggcg gcggggtcgg cgggagtgcc
       61 gaagacgtcg gtggcgggtg ccgcgccggg tccggcggcc tgctgcaccg gcgcgcaggc
      121 cactgtgagg gccgcggcca gtgcgatccc cagcgtcacc gccccgcgcg cccaaatctt
      181 tggtgctgcc gtgctgagcc tcatcgttct acctccggcc gttcattcgg tcgggtgaac
      241 gtagtgtgta tacacacgag aaaaccatgg cggcgacgca gtcttgacct agccgaaaca
      301 cgaagagtgt tgcgtccgtt gctcgctgtc tccgggcggc tgcgcggtcg ccggcgggca
      361 gcgtccccgc cacaatgagt gcatgactgc cgagctcccg gccggctgag tgcctgccct
      421 cgctcccgcg gagcagcgcg cacggttggc ggcggcctcg gttgcgaggc tggccaccgt
      481 gcgggccgac ggcaccccgc gactcgtgcc gatcacattc gcgctggtgg aggggctcgt
      541 ctgctccgtc gtggacgagg tgaagcccaa gcgcagcccc cggctcgccc ggctcgacga
      601 cgtgcggcgg gacccgcggg tcgccctcgt cgtcgaccac tacgaggacg actggtcggc
      661 cctgtggtgg gtgcgggtgg acggcacggc cgagatccac gaggccggac cgctgcggga
      721 gcgggcactc gccgagttgc gggccaagta cccgccctac gccgccgcgc cgcccgcggg
      781 tgcgctgctg gtgatcacgc cgtcgcgctg gagcggctgg accgcggcgt gagtcaagag
      841 ggcaccagga ccacggcctc gatctgcacc gcgatggacg aggagattgc ggtcgctgtc
      901 gatcaccgcg atcggcgagc tgctcggcat ccccctggac gagcgccggc agctccgccg
      961 atgggccgac acggcccgtc gaagccgcca accgcgaccc cgacccgctt cccgaccccg
     1021 acgcttcgac cccgcccggc ggcccaaccc ccatctcgcc ttcggtcacg ggcagcacca
     1081 ctgcctcggt gccgcactgg cccgactcga gatcggggtc gccctccgcg cactcgcggt
     1141 ggcgctcccc gggctccgac cccgcgaccc catcggggac atcccgtgga tccacggcct
     1201 cgtcgactcc ggaccggccg caatccggat cgcccggccg gtcaccgtcg ccctgccccg
     1261 ggagcgaacg gcggagatca ccaccgtgtg ccttcgcgtt cgtgccgggg tggcccgctg
     1321 actcagaggt tgccccgctt ctcctgctcc cgctcgatcg cctcgaagag ggccttgaag
     1381 ttgcccttgc cgaagccgag tgagccgtgc cgctcgatga gctcgaagaa cacggtgggc
     1441 cggtcctgca ccggcctggt gaagatctgc agcaggtagc cgtcctcgtc gcggtcggcg
     1501 aggataccca gctcgcgcag ggtctcgaca ggcacgcggg tgtcgccgac ccagtcggtg
     1561 agggtgtcgt agtaggagtc cggcgtcgcc aggaactcga cgcccatcgc gcgcatcgcc
     1621 cgcacggacg cgacgatgtc gccggtggcc agcgcgatgt gctgcacgcc cggcccgccg
     1681 tggaactcga ggaactcgtc gatctgggag cgcctccgcc cggcggccgg ctcgttcagc
     1741 gggaacttga ccttgtggtt gccgctggcc acgaccttgc tcatgagggc cgagtactcg
     1801 gtggcgatgt cctcgccgac gaactcggcc atgttggtga agcccatgac ccggttgtag
     1861 aagctcaccc agtggtccat gcggcccagc tcgacgttgc ccaccgcatg gtccacggcc
     1921 tggaagcacc gcttcccggc cgccgggaac aggggctcgc gcgcgacgaa acccggagcg
     1981 aacggaccgt ggtaccgcga ccggtcgacc agggtgtgcc ggacgtcgcc gtaggtggcg
     2041 atcgccgccc tgcgcagctc gccgtgctcg tcggacacgg tgtgcggctc ggtcagcacc
     2101 atcgcgccgg cggcgcgggc gtgcgcgacc gcggcgtcga cgtcggggac ggtgatcgcg
     2161 aggtcggcca ccccgtcccc gcgctcggcc acccgcgcac cgacccaggt gcccgcgtgc
     2221 acctcacccg cgagcacgaa cctggccgac cccgaggtca gcacgtactc ggcgagatcc
     2281 cgatggcccg tctccggccc acggtaggcg accacgcgca tcccgaacgc cgacgagtac
     2341 cagtgcgcgg cctgccgggc gttgccgacg gcgaagcgga cgtggtccag tcccagcacc
     2401 gggaacgcgt cggtggagtc gtcgtgagcg acctcgccga tgagcgggtc gatcagcgcg
     2461 tcggtgatgc cgccagtggt catgccggcc agtagaagtt ctgctgtgcg ctgtgacaag
     2521 ctcctgtagc tgagctatgc gctccgcatg gatgacatgg gcgacgggag caggagatga
     2581 ccacaccggc cagctcgccg gatgcgctcg acgtcgagct catcgggctg ctctccgagc
     2641 acccccgggc gggcgcgctg gagctctcgc ggctcgcccg cgtggccagg gccaccgtca
     2701 ccgcgcggct ggagcgcctg gaacgggccg gggtgatcac gggttacgga cccgacgtcg
     2761 acctgcgtgc cgccggcttc ccggtgcagg cgttcgtgtc gctggaaatc gcccaggggg
     2821 ccatcgccga cgtccgcgcc gagctcctcg ccattccggc ggtgctctcc gcgcacgcca
     2881 ccaccggtac cgcggacgtg ctctgccgcc ttgccgccgc ttcccacgag gacctgcacg
     2941 ccacgttgct gcagatcgac cgctcaccgg ccgtgctgcg ctcgaccagt gtcgtcgtcc
     3001 tgtcggaggt ggtggcgtcc cggtacctgc cgctgctgcg cggcacgggc cgaccgcgcc
     3061 cgagccgggc gccggcgtac cgggagtgct gacccaggtc agcctgtcgg tgcgcacaca
     3121 gcccgcacga acgctgcgat cgccgggtgc gagctggatc cgcgtcggca ggccgccggc
     3181 gtgctgcgcc gcatggggag cggcgtaagc accacgtccg gtacggactc ggcggctgcc
     3241 agttgtggta cgagtgccac gccgcccccg agcggatcgc gctcagcact acgggagagc
     3301 ccgcgaccct ggacgacagc gagcgccgga cggttgtgga cgtctgcgca atggcgtgcc
     3361 gcgacgcggg agctgcgttg atcgtcgggg ccggcacgga cccctacggc agcttgctga
     3421 cgaaggccgt cacggcctcc tccgtgtctt cgggccgctg caggtgcgga gagtggccgc
     3481 agtcgagcac cagcagctcg acgggcccgg tcgcggcgtc ccgcaccgcc tcgacgtgcg
     3541 cgagcgtgcc gtactcgtcg gccgtgccct gcaccgccag caccgggcag gtgatcgcgg
     3601 gaagctcgaa gcggatgtcc cagtcgcgga acgcgtcgtc gagccagacg tcgttccagt
     3661 tccagaacgt cacgtccggg tcgcgatggt ggcgggccat cctcgcccgc aggtcgccct
     3721 cggtgtaggc gcggcgggcc tgctcgatgc cgccgaggcc gatctcctcc acgaactcat
     3781 gcggggccag caccacgagc ccggtgacgt ccgagctccg tgcggcgtac agcagcgcga
     3841 tcgaggcgcc gtcgctgtgc ccgaccaaca cggggcggtc cagcccgagg gccgcgcaca
     3901 gcgccggcac caccgtggcg gcctcgtcgt gcatgaagcg gggcgtccgc ttctgcgtcg
     3961 gcaggtccga gaacccgtgc ccgagccggg agtaggccac cgcacggcgc ccggttgcgg
     4021 cggcgatccg atggtggaac gtgcgccaga ggcccagcga gccgaggccc tcgtgcagga
     4081 acagcagcgg cgcgacggtg gggtcacccg gcacgtcctc gtactcaatg cgcccaccgg
     4141 gcacctcgac acaacggcgc tcggccacgc caagagccta gggcgtgccc gaccctcatc
     4201 ggaggggaag ggggaggtgg tggtcagcgg ttgagccgcg cgaggagctt cttcggcgcg
     4261 atcacccggt aggcgtcggt gacgagctcg gcgatctcgt cccagtccac gtcgtcgaga
     4321 tcgaggcaca cccccaccca gccccgccca cccacgtacg gcggccggaa gaagcgcgtg
     4381 ggagccgcgg ccacgagctc ctcctgggtg ccgggttgcg cggcgcacca gaacgccacc
     4441 cggtcgtcgt ggtgctgatc ggcgtacgtc acgaacatct tgcgcacgaa ccaggtcggc
     4501 tccccgtggc tcaccttctc ggtgacctcc ggcagagcca ggcacagggc gcgcaggcgg
     4561 gcgagcagat cggtcacgcg tctccgaccc ctttcacctg gggccggtag ctgccgaccg
     4621 accagacgtt gccctcggga tcgagcaccg cgtactcccg tgagccatag ggctggtcga
     4681 cgaggccctg cacgacctcg gccccggcgg cgacggcccg gtcgtggtgg gcgtccacgg
     4741 cgtcggctcc gtcgaggacc aggtagatca ccgcccggcc ggtgtcgaac gggccgggcg
     4801 ggtcgctcct ggtgccgagc atgaccacgc cgtcgccgaa gctcagctcg gcgtgcccga
     4861 cagtgccgtc gtcggcggtg tggacctgct cctcgacgag gccgagtgcg ccggtcagga
     4921 acgcgatcgc ggccttcgaa tcgttgtaaa gaagggtggg gtggatgctc atggcgcgac
     4981 gttaggccgg gtcagtctgc ctgggcttga acgaatggga accgtcagct ccgttcggcg
     5041 aggtactccg tgggggtgca gccggccagc gtccggaact cgcgtacgag atgagcgtgg
     5101 tcggcgtacc cgcaggtggc ggcaacatcg gcgaccgagc gggccgcccg ggcgcgcgag
     5161 ccgccgtccg ggccgggcgg cagcagcagc tcggccgccc gctggaaccg cagcacccgc
     5221 gccgcgggct tgggtgcgag gcccacctgc atacggaacc ggttcaacag atgacgccgg
     5281 ctccagcccg taccgtcggc gagctgctgg atgccgatcg cgcccgcggt gcgcaccagt
     5341 cggtcccacg cccaggcgac ttcggggtcg ggccgggcgc gcgaggcgtc caggcggcgt
     5401 agcaatgcgt cgtcgacgcg atcgaagcgc tccggccacc ccggctcctc ggcgagccgg
     5461 gcgggcaggg cggtcagctc gggcacctcg agttcgtcga cgtggggcac gcggttggtg
     5521 agctccggca tcggccgccc gagcagggtg aacaccccga gcggggtgag gtcgacctgc
     5581 accccgtgct gcacgccggt gaactcggtg atcacggcgg catcgtgcat cccggccagg
     5641 aaggagaccg ggacggtcgg acccgcgggc ccgtggagcc ggatgggcgg gccgaggctg
     5701 aggatgagcg tgcacgagcc ggtgggtgcc tgcctgcggc gcacgggcgc ggcgctgcgc
     5761 tcgcggtagc ccgagtaccc caggacatgc tcggcgagcg ccgggtgcgg ccgcccccgg
     5821 acgtactcgc cgacgggtgc ctccaccagc ccagcatggc agcagaacgc cgctcggacc
     5881 tacgctctgc accgtggacc ggcccgacga cctcctcgcc gagctcgtcg gaatgcttcc
     5941 cgtcggtatc ttcgccgtcg acggcgacgg ctgtgtcctg ctgtggaacg ctgcggccga
     6001 acggctcacg ggctggtccg gcagtgacgt gatcggacag cgcgtgatgg gccccgaccc
     6061 cacccgccgc acgttcgacg cgccgaccgc cgcccgcatt ctcgacgagc tccgggcggg
     6121 gcggacgtgg agcggcaggg tgcccgccac ccccgtcagt gggcgcacgc tctatttccg
     6181 cgccgcaccc ggacccggct cgacgatcgt cggggtgctg caggacgtcg acgacacgga
     6241 g
//
LOCUS       DASRSC010000276        10508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1583771,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000276 DASRSC010000000
VERSION     DASRSC010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10508
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1583771"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VFE39_13570"
     CDS             <1..205
                     /locus_tag="VFE39_13570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259673.1"
                     /translation="VIRTLRTAAARCAPSTRPDLVLGLMSEPTPTVGYTSMLSSIPFP
                     ARALRPLLIARFHNRKRKLGLAA"
     gene            497..940
                     /locus_tag="VFE39_13575"
     CDS             497..940
                     /locus_tag="VFE39_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259674.1"
                     /translation="MLTGVLVTVGALLLWRIRPSSWLNDTALVVLVIMGLGKILLGFA
                     PENTDVGLHLMGALNVPLGSMAVLLLSLVMRRTDRGLSAIGMERVATYPAPLWLLWTC
                     RTGAVTRDQPPAAPRRRVPAFPGPRIPDPVFAVERVWNYSSWASS"
     gene            complement(916..1092)
                     /locus_tag="VFE39_13580"
     CDS             complement(916..1092)
                     /locus_tag="VFE39_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259675.1"
                     /translation="MSSDHEYSGDYGYDLLHEVRATVFIPAARTPLPVHGARLPGREL
                     DPDGDLGYDEAHEL"
     gene            1295..1432
                     /locus_tag="VFE39_13585"
     CDS             1295..1432
                     /locus_tag="VFE39_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259676.1"
                     /translation="MLRIERATAGTITLTVNDVDHSLDIDHRTTLLDALRERLDLAGT
                     K"
     gene            1511..1840
                     /locus_tag="VFE39_13590"
     CDS             1511..1840
                     /locus_tag="VFE39_13590"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HET6259677.1"
                     /translation="MAVAGKAATTIEGIGGAATGDALHPVEQALLDHDGYRCGYCTPG
                     QICSAVAVLDEHAAGWPSAVTGDVGAEEIVLDEPEIRERMSGNLCRCGAHVSIVAAVA
                     DAAGARP"
     gene            2408..3202
                     /locus_tag="VFE39_13595"
     CDS             2408..3202
                     /locus_tag="VFE39_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6259678.1"
                     /translation="MDLELTGKRAVVSGGSRGIGFAVADRLAAEGADIALVARDPVAL
                     EEAAARIGRHRATVLVHPADTRDDAAVRAMVDDVVATLGGVDILVNAAAQPAGGPVPP
                     LAELPDDALRAEVETKVLGYLRCARAVAPHMSAQGWGRIINISGLAARQTGSIVGSIR
                     NVAVAAMTKNLADELGPCGITVTAVHPGMTVTERVPELFARLAERDGVTEQDVAARFA
                     AATSIGRLITAEEVADVVTFLASPRSVAITGDAIPVGGGSRGTIHY"
     gene            3281..3724
                     /locus_tag="VFE39_13600"
     CDS             3281..3724
                     /locus_tag="VFE39_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6259679.1"
                     /translation="MDTRQTATELLERIALGDPEKIAELYAERVDWKLDWPPGDPSRS
                     VPWIQDRSTRAGVAEHFRLIAEHHVTGQSSAEIEKLLVDGPDAVVLGELRNTAKPTGR
                     SYVAAFALHLTVENGLISRHHVYEDSLAVWNAFDAVPAPPAVIGA"
     gene            complement(3750..5420)
                     /locus_tag="VFE39_13605"
     CDS             complement(3750..5420)
                     /locus_tag="VFE39_13605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HET6259680.1"
                     /translation="MSTTFEPGPVASTRRWQAGLVDQLLGRLMRLPAGSGYTVRRDLR
                     VPMRDGVQLLADHYEPATSPALGTVLVRGPYGRAALYALLYARLYAARGYHVLLQSCR
                     GTFGSGDSFQPMLREIDDGHDTVAWLRDQPWFDGRLATMGPSYLGFTQWALLMDPPPE
                     LRTAVVSMAPHDFSRSTHGTGAFTLGDFLGWSDLVAHQEETGFVRGLVRSATAGRRLK
                     PGLDGLPLGGAGEEVLAGRASWYRDWVSRPDPGDPYWKPMQLVEALDRVQVPVLLIGG
                     WQDLFLDQTLDQYAHLHRRGVDVALTVGPWTHIDLVTKAAPTTARETLDWLGEHLAGS
                     PGRRRPAPVRIYLTGADGGWRGLPEWPPTADEEVLYLQSDGGLGPDDPPTGAAPSRFT
                     YDPARPTPTVGGRVLAGASGIRDNRALEARPDVLTFTGAVLAADLEVVGAPVVELAHH
                     SDNPHADVFVRICEVDANGRSRNVSDGFVRLDAADPTEVVRVELDAVAHRFGAGHRIR
                     LQISGGAHPRFARNLGTEEPAAAATRMVPSHRTITLGGSRVVLPVAAR"
     gene            5649..5870
                     /locus_tag="VFE39_13610"
     CDS             5649..5870
                     /locus_tag="VFE39_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259681.1"
                     /translation="MWIRLTVSESPVFRKALGAKSAARMPVLDALRAYPKEIALASGS
                     FLISLSCSYLMTETRGRGLSAEEPVRAHS"
     gene            complement(5962..6543)
                     /locus_tag="VFE39_13615"
     CDS             complement(5962..6543)
                     /locus_tag="VFE39_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009084681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HET6259682.1"
                     /translation="MRATPLLIAALLCVPIAACGGGGGASDRAAPPAASPGSVEASGT
                     FAAPPGGVVTYDEALVPVGATAAVDQTAAGGSTTVTLDVAGLMPNRQYGAHAHANPCG
                     AEAKDAGPHYQLTPDPVTPSVDPAYANPHNEIWLDFTTDEQGAGRATSTVAWEFPADR
                     RAKSVVIHTMPTATEPGKAGTAGDRAACITVGF"
     gene            complement(6596..7345)
                     /locus_tag="VFE39_13620"
     CDS             complement(6596..7345)
                     /locus_tag="VFE39_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259683.1"
                     /translation="MGGVAEQDGPTPVPPQERAHREQPPPDAVPNRPDHLDDPRMPAL
                     ERPDGCVLVHLVHPAVRRLPRLRSFDDREEVHDVAGTADRVVDHVCVGAHPELDGVRV
                     GEGRYPVDGYEAVEGAGAGVDPVGSNLVAVAGDEVPHGRAHTVGADHEVRVLDAPVRE
                     FEDDSVGSLDEIDEIDEPASQMHGFRRRERRPGSSADARDESRRCETEAQAPDTAADD
                     RHRVYRSHTQRVEVSAAMYVRLCICGVHRDP"
     gene            7767..9767
                     /locus_tag="VFE39_13625"
     CDS             7767..9767
                     /locus_tag="VFE39_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HET6259684.1"
                     /translation="MTRSEQRGSAMKPFVMNQHGKMVFPANVLGALDFSVLDTLDQFT
                     SVIGRDFEAKAPTGADILARVESGAYPDRFTLLRDLGQNLYWVNRYVLPMFDKRPTRW
                     RDLPRHRTDVFLPLLTPWEDADRKIAAVERGYGELPATWDAPAEDRIFALLFDVFRHK
                     RHHATELPAIPPTVGEMLARPEALTLVLPRHDPDYRVFTFEEILDADEEVPELEALLR
                     WAKVLHNQYPWQRADGALRPVSTIGDDEFVIAYHPRDREVVAFIDRVRAAETDGQRAA
                     APPTVSPRVDARQPVRPYPPVRVREAFGVAPKLEALSVVRGEHICTNADVIRNSSFSW
                     SPMSAAEISAKTGIDQRCYSARPLEQLAFDAARAAIAHSGRAPEEFGAVLVATCTSNR
                     LIPSIACWLSGELGMLQTHASADIVAACAGLPYGLAEAVRQLQEVHRPVLLVCAEKFS
                     DKIGSVRTSRMIFGDGASAMVVAPAEPGEVGDVQVLQTYASGPASEVNSIIWPNPEFD
                     NDITVYGPEVKSLVRRYLAQMISELDEQGDPDSPGRSLLESIELIVPHQANKTMIVDL
                     ARKAGLAEEQLYFNIATMGNVSAASIPIALHDAVGDGVIKRPTRVFAPGFGAGAVGGY
                     AVLTIDPAIIAPEAEPNTAPGIAGLLTPIATTSDDVRIAFGD"
     gene            9873..>10508
                     /locus_tag="VFE39_13630"
     CDS             9873..>10508
                     /locus_tag="VFE39_13630"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HET6259685.1"
                     /translation="MTDQKVAIVTGGARGIGAAITDSLARSGVHVAAGYSSNSRAAEE
                     LAGKLSAEGCSVSVHQGNVGVPEDCQRVVEEVLADRGRVDHLVNNAGVTVDKTMRKMT
                     VDDWHAVLRTNLSGAFYMSKAVLEHMLERGSGRIVNISSVIGQTGNVGQVNYAASKSG
                     LFGMSQSLAREVARKGITVNCVAPGYVETEMVAAVPENVLEKIIAGIPVGRL"
BASE COUNT         1535 a         3688 c         3751 g         1534 t
ORIGIN      
        1 ggtcatccgc accctgcgaa cggcggctgc gagatgcgcc ccctccacgc gtcctgacct
       61 ggtactcggg ctcatgtccg agccgacccc cactgtcggc tacacgtcga tgctgagcag
      121 tatcccgttc cccgcgcgcg cgctgcgccc gctgctgatc gcgcggttcc acaaccggaa
      181 acgcaagctc ggtctcgccg cgtagcgacc ccgccccatc ccggcgcctg accctcgcac
      241 gtgcttgccc gctgcaatgc ggacggccga cgcagcatga gacaggtcgg tccggggggc
      301 cgctgcaagg ggaacggcgt gaccactgcc ggtcctcggc gcggggcgtg gccgaggctc
      361 tggtcgggcg ccgagaactt cttccaacgc gcggggcttc cccagcacca cctgcaacct
      421 ctcgacgatc aggcggcccc tcgtgggaac ccgttcccga tacgtcctgt tcgcggccgc
      481 gtagaacggg tcgttcgtgc tcaccggcgt gctggtgacg gtcggggcgc tcctgctgtg
      541 gcggatccgg ccgtcgtcct ggctcaacga cacggccctg gtcgtcctgg tcatcatggg
      601 gctcggcaag atccttttgg ggttcgcccc cgagaacacg gacgtcggcc tgcatctgat
      661 gggcgcgctg aacgtcccgc tcggcagcat ggccgtactc ctgctcagcc tggtgatgcg
      721 ccggaccgac cggggcctca gcgccatcgg gatggaacgt gtcgccacct atccggcgcc
      781 gctgtggctg ctctggacgt gcagaacggg cgcggtgacc cgggatcaac cgcccgcagc
      841 cccccgtcga cgtgtcccgg catttcccgg gccacgcatt cccgatccgg tgttcgccgt
      901 ggagagggtg tggaactaca gctcgtgggc ctcgtcgtag ccgaggtccc cgtccgggtc
      961 gagctctcga ccggggaggc gagccccgtg aaccggcagg ggcgtgcgtg cagcgggaat
     1021 gaaaacggtg gccctgacct cgtgcagtag gtcgtagccg tagtcacccg agtactcgtg
     1081 atcgctcgac atcaacgtcc cccttgcggc tcaccgattc tcacgctccg tgatcggagt
     1141 gctccttacg gtgcagagag atcatcacct gactggtgtg ggcggggcaa gagcgatcct
     1201 ccgagtggtc ggggggcgag ttcaggagcg cgtagcgggt gtagtagtgg accccactgc
     1261 tgacaggaca cacgccagga cctgacaccc ggaggtgctc cggatagaac gcgctacggc
     1321 cggcacgatc accctgaccg tcaacgacgt ggatcactct ctcgacatcg accaccgcac
     1381 gaccttgctc gacgcgctgc gcgagcggct cgacctggcc ggaaccaagt aggggtgcga
     1441 ccacgggcag tgcggcgcgt gcacggtcct gatcgagggc cgccgggcca actcgtgcct
     1501 gctgctcgcc gtggccgtcg cgggcaaggc ggccacgacc atcgagggca tcggcggtgc
     1561 tgcgaccgga gacgccctgc acccggtaga gcaggccttg ctcgatcatg acggctaccg
     1621 gtgcggctac tgcacgcccg gtcagatctg ctcagcggtc gcggtcctcg acgagcacgc
     1681 ggcgggttgg cccagcgccg tcaccggcga tgtcggcgcc gaggagatcg tgctggacga
     1741 gccggagatc cgggagcgga tgagcgggaa cctgtgccgg tgcggagccc acgtgtcgat
     1801 cgtcgcggcg gtcgcggacg cggcgggggc acggccatga gggagttcgg acgcgacccc
     1861 gggcccgggc ccgggtgcgc cgacttcggc caggcccagc tcgccggggg ctcgctcggc
     1921 accgggtcgt ggggcttcgc gatcaccgag gcccgcgggg cgttgcgcac cgagctcgac
     1981 ggcggggcga ccgtgccgcc cgacggcctg accgcgcgcc ggtgttcggc cacgtcgtca
     2041 accacgacct ggccggctac cacgtggccg cgcacgccga cgtcccgccg atcgaggtca
     2101 gcgctgggtg gacgacgcgg acggtgcttc tttcgtagcg cccgcccgac ctgccgaggc
     2161 gttagccgcg cccacgatcg ggcaccgact gccgctcgtt caagcaggcc gaggcgctcg
     2221 ggttgtccgc cgtcgacggg cgtgctgggc agcgcagcag tccgacgagc tcccgcgcgc
     2281 tgtgtggtcg cggtcctcct tccgagcagc gccgcccacg tcccggccgc cagctccatg
     2341 gctggaatgc gatcgtggac gaggggcgtc gcgcgctggg ccgaccgtcc gctgtaggga
     2401 ggatcaggtg gatctcgaac tcaccggcaa gcgcgccgtg gtgtccggcg gtagccgcgg
     2461 catcggcttc gccgtcgccg accggctcgc cgcagaagga gcggacatcg cgcttgtcgc
     2521 gcgagacccg gtggcgctcg aggaggccgc ggcgcggatc ggacggcacc gggccaccgt
     2581 gctcgtccac cccgccgaca cacgggacga cgcggcggtg cgggcgatgg tcgatgacgt
     2641 cgttgccacg ctgggtggcg tcgatatcct ggtgaacgcc gcggcacagc ctgctggtgg
     2701 cccggtgcca ccgctcgccg aactgcccga cgatgcgctc cgggccgagg tcgagacgaa
     2761 ggttctcggc tacctccggt gcgcccgcgc cgtggcaccc cacatgagcg ctcagggctg
     2821 gggccgcatc atcaacatca gcgggctcgc cgcccgccag accggctcga tcgtggggtc
     2881 catccgcaac gtcgcggtgg ccgcgatgac gaagaacctc gccgacgagc tcggtccgtg
     2941 cgggatcacg gtcacggccg tgcatccggg gatgaccgtc accgagcgcg tgccggagct
     3001 cttcgcccgc ctggccgagc gggacggcgt caccgagcag gacgtcgccg cgcgtttcgc
     3061 cgccgccacg agcatcgggc ggctgatcac cgcagaggag gtggcggacg tcgtgacgtt
     3121 cctcgcctcg ccgcgcagcg tcgcgatcac cggcgacgcc atccccgtgg gcggcggcag
     3181 ccgcggcacc atccactact gaccggagca gcgaccggcg gcgggccgca tgacctccga
     3241 ggtcatcgac gggggtcgtc cagtcgacca gagtggtgcg gtggatacga ggcagacggc
     3301 cacggagttg ctggagcgga tcgcgctggg cgatcccgag aagatcgcgg agctctacgc
     3361 cgagcgggtg gactggaagc tggactggcc gcccggcgac ccgtcgcgat cggtcccctg
     3421 gatccaggac cggtccacgc gcgccggagt ggccgagcac ttccggctca tcgccgagca
     3481 ccacgtcacc gggcagagct cggccgagat cgagaagctg ctggtcgacg gccccgacgc
     3541 ggtggtactc ggggagctgc gcaacacggc gaagccgacc gggcgatcgt atgtcgccgc
     3601 cttcgcactg cacctgaccg tggagaacgg attgatcagc cgccaccacg tctacgagga
     3661 cagcctcgcg gtgtggaacg cgttcgacgc ggtgccggcg ccaccggctg tcatcggcgc
     3721 gtagcaggag gacgccggcc cgcgagcgat cagcgggcag ccacgggcag caccacccgg
     3781 gacccgccga gggtgatcgt gcggtgtgac gggaccatcc gggtcgctgc cgcggctggc
     3841 tcctccgtgc ctaggttgcg ggcgaagcgc gggtgggcgc cgccgctgat ctgcagccgg
     3901 atccggtggc ccgcgccgaa gcggtgggcc accgcgtcca gctccacccg cacgacctcg
     3961 gtggggtccg ccgcgtcgag ccggacgaag ccgtcgctca cattgcgcga gcggccattg
     4021 gcgtcgacct cgcagatccg gacgaacacg tcggcgtgcg ggttgtcact gtggtgggcc
     4081 agctcgacga ccggggcacc gacgacctcg agatccgctg ccagcaccgc tccggtgaac
     4141 gtcaggacgt ccgggcgggc ctcaagcgcc cggttgtcgc ggatgccgct ggcgcccgcg
     4201 agcacccggc ccccgacggt cggggtaggg cgggcggggt cgtaggtgaa ccgtgacggc
     4261 gccgcgccag tgggcggatc gtcgggcccc agcccaccgt cggactggag gtacagcacc
     4321 tcctcgtcgg cggtgggtgg ccactcgggc agtccgcgcc acccgccgtc ggcccccgtc
     4381 aggtagatgc gcaccggtgc gggtctgcgg cgcccggggc tgcccgcgag gtgctcgccg
     4441 agccagtcga gcgtctccct cgcggtcgtg ggcgccgcct tcgtcacgag gtctatgtgg
     4501 gtccaggggc caacggtgag ggccacgtcc acgccgcgcc ggtgcaggtg ggcgtactgg
     4561 tcgagggtct ggtcgaggaa caggtcctgc cagccgccga tcagcagcac cggcacctgc
     4621 acccggtcga gcgcctcgac gagctgcatc ggcttccagt acgggtcgcc cggatccggg
     4681 cgcgacaccc agtcccggta ccaggacgcg cggccggcga gcacctcctc ccccgcgccc
     4741 ccgagcggca ggccatcgag gcctggcttc aggcgccgtc ccgcggtggc ggagcggacg
     4801 agacctcgga cgaaccccgt ctcctcctgg tgggcgacca ggtcgctcca gccgaggaag
     4861 tcgccgaggg tgaaggcacc cgtgccgtgg gtcgagcggc tgaagtcgtg cggtgccatg
     4921 ctgaccacgg cggtccggag ctccggcggt gggtccatga gcagcgccca ctgcgtgaac
     4981 ccgaggtagg acggccccat cgtcgcgagg cgcccgtcga accacggctg gtcccgcagc
     5041 cacgccaccg tgtcgtggcc gtcgtcgatc tcgcggagca tcggctggaa ggagtcgccc
     5101 gacccgaagg tgccgcggca gctctgcagc agcacgtggt agccccgagc ggcgtacagg
     5161 cgcgcgtaca gcagcgcgta gagcgcggcc cggccgtacg ggccgcggac cagcaccgtc
     5221 ccgagtgcag gcgacgtcgc gggctcgtag tggtccgcga ggagctggac gccgtcgcgc
     5281 atcgggaccc gcagatcccg gcgcacggtg tagcccgacc cggcgggcag ccgcatcagc
     5341 ctgccgagca gttggtcgac gagcccggct tgccagcggc gcgtgctggc gacgggtccc
     5401 ggctcgaagg tcgtactcat ccgttgcctc ctggcgggtc aacactcact gacccgagcc
     5461 tcgtcggtga tgttcggggc gtccagcacc ggcgcgcggt tgatgtccgc cgggaacggg
     5521 gattccagga gccccgagcc gtgcccagcg gccgggtcag tcctgcgagg accgttccat
     5581 ggcagcgccc gagaacccgg gacaccaccg cccgactcct cgcacggcgg ccccctctct
     5641 ccgtctctat gtggatccgg ctgaccgtca gcgagagccc ggtcttccgg aaggccctcg
     5701 gtgctaaatc cgcggcgcgg atgcccgtac tcgacgcgct gcgggcctat ccgaaggaga
     5761 tcgcgctggc ctccggctca ttcctgattt cgttgtcctg ctcgtatctg atgaccgaga
     5821 ccaggggtcg cgggctgagt gccgaggagc cggtccgcgc ccactcctga cctcgccgaa
     5881 accgactgaa gagtgcgccc gcgtcggagc cgaccaccac tcccacgtcg acctcgatcg
     5941 ctcagcgccc cacccgcgat ttcagaagcc cacggtgatg caggccgcac gatcaccggc
     6001 ggtcccggct ttacccggct cggtggcggt cggcatcgtg tggatgacga ccgacttcgc
     6061 gcggcgatcc gccgggaact cccacgccac ggtcgaggtc gctcgacccg ccccctgttc
     6121 gtcggtggtg aagtcgagcc agatctcgtt gtgggggttg gcgtaggccg gatcgacgct
     6181 cggtgtcacg ggatcgggcg tgagctggta atgcgggccg gcgtccttcg cctcggctcc
     6241 acacgggttg gcgtgcgcgt gcgcgccgta ctgccggttc ggcatgagcc ccgcgacgtc
     6301 cagcgtgacc gtcgtgctgc cacctgccgc ggtctggtcc acggcggcgg tcgcgccgac
     6361 cgggacgagc gcctcgtcgt aggtcaccac gccccccggc ggggcggcga atgtcccgct
     6421 cgcctcgacg ctgcccgggg acgccgccgg cggggcggct ctgtcggatg cgccaccgcc
     6481 acctccacac gcggcgatcg ggacgcacag cagggcggcg atgagcagtg gtgtcgcgcg
     6541 catgccgagc aggatggcgc atttcaggcc agtcggcagt cctcgtccgg aatgatcagg
     6601 gatcgcggtg gacaccgcag atgcataatc gaacgtacat cgcggccgat acttctactc
     6661 tttgtgtatg gctgcgatac acacggtggc gatcatcggc ggcggtatcg ggggcctgag
     6721 cctcggtctc gcacctacgg gattcatcgc gcgcatctgc agaggatcct gggcgacgct
     6781 ctcggcgacg aaaaccgtgc atctgggacg ccggctcgtc gatctcgtcg atctcgtcga
     6841 gcgacccgac ggaatcgtcc tcgaattcgc ggacgggcgc gtcgagcacg cggacctcgt
     6901 ggtcggcgcc gacggtgtgc gctcgaccgt gcggcacttc gtcaccggcg acagcgacga
     6961 gattggatcc gaccggatct actccggcac cagcgccttc cacggcctca tacccgtcga
     7021 ccggataccg accctccccg acccgcacgc catccagttc tggatgggcc ccgacgcaca
     7081 cgtggtccac tacccgatcg gcggttccgg cgacatcgtg aacttcctcg cggtcgtcga
     7141 aggaccgcag tcgtggaagc cttcggacgg ctgggtggac gaggtgcacg aggacgcacc
     7201 cgtcagggcg ttcgagggct ggcatccgag gatcatcgag atgatcgggg cggttcggca
     7261 ccgcgtccgg tgggggctgt tcgcggtgcg cccgctcctg cggtggtact ggggtcgggc
     7321 cgtcctgctc ggcgacgccg cccacgccat gctcccgcac caggggcagg gcgcgaacac
     7381 caccgtggag gacgccttcg ctctggccgc gctcctcgcg gatgccacgg cggccgggct
     7441 gcccgctgtg ctcgcctcct accaggcact tcggcgggcc cggacccgac agatccagct
     7501 cagctcctgg gcgaccagcg ccctgctcca ccttccgagg gccctgctgc cgcggaacgc
     7561 gacctcagga ccgcccaggt gccgcaggac gtcgggtgga tccacgagta cgacgtccag
     7621 gaggcactgc gcaggcggcg gctgccgccc gagcccgtgt agcgcccagt cggctcgacc
     7681 ggcgaacccg gcggccggat ctccgcacgc cgttgccgga ttcccccgct cgtcggttgt
     7741 gcgccggtcg gcccggaggg ttgagtgtga cccgttccga gcaacggggg tcggcgatga
     7801 agccgttcgt catgaaccag cacggcaaga tggtgttccc agcgaatgtc ctcggggcgc
     7861 tcgacttctc cgtgctcgac actctcgacc agttcacgag cgtgatcggc cgcgacttcg
     7921 aggcgaaggc cccgaccggt gccgacatcc tcgcccgcgt ggagtccggc gcgtacccgg
     7981 atcgcttcac gttgctgcgc gacctgggcc agaacttgta ctgggtcaac cgctacgtgc
     8041 tgccgatgtt cgacaagcgc cccacccgct ggcgcgacct gccgcgccac cgcacggacg
     8101 tcttcctgcc cctgctcacg ccatgggagg acgccgaccg gaagatcgcc gccgtcgagc
     8161 gcggctacgg agaactgccc gcgacgtggg acgcccccgc ggaggaccgc atcttcgcgc
     8221 tgctcttcga cgttttccgg cacaagcgcc accacgcgac cgagctgccg gcgatcccgc
     8281 cgacggtggg cgagatgctc gcccggcccg aggccctgac cctggtcctg ccccgccacg
     8341 acccggacta ccgggtgttc acctttgagg agatcctcga cgcggacgag gaggtgccgg
     8401 agctcgaggc cctgctgcgc tgggcgaagg tgctgcacaa ccagtacccc tggcagcgcg
     8461 ctgacggggc gctgcgcccg gtgagcacga tcggtgacga cgagttcgtg atcgcctacc
     8521 acccgcgcga ccgggaggtg gtcgcgttca tcgaccgggt cagggctgcg gagaccgacg
     8581 ggcagcgggc tgcggccccg ccgacggtct cgccgcgggt ggatgcccgg cagcccgtcc
     8641 ggccctaccc gcccgtacgg gtgcgggaag cgttcggcgt ggcaccgaag ctggaggcgc
     8701 tgtccgtcgt gcggggcgag cacatctgca ccaacgccga cgtcatccgc aactccagct
     8761 tctcctggtc accgatgagc gcggccgaga tcagcgcgaa gacgggcatc gatcagcgct
     8821 gctactccgc tcgtccgctc gaacagctcg cgttcgacgc ggcgcgtgcc gcgatcgcgc
     8881 actccgggcg ggccccggag gagttcggtg cggtgctcgt cgccacctgc acgagcaacc
     8941 gcctgatccc gtcgatcgcg tgctggctct cgggcgagct ggggatgctc cagacgcacg
     9001 cgtcggccga catcgtcgca gcgtgcgcgg gcctgcccta cgggctcgcg gaggcggtgc
     9061 ggcaactgca ggaggtgcac cgtcccgttc tgctggtctg cgccgagaag ttctccgaca
     9121 agatcgggag cgtgcgaacg tcacggatga tcttcggcga cggcgcctcg gcgatggtgg
     9181 tggcgcctgc ggagccgggc gaagtgggcg acgtgcaggt gctgcagacc tacgccagcg
     9241 gtccggccag cgaggtgaac tcgatcatct ggcccaaccc ggagttcgac aacgacatca
     9301 ccgtctacgg gcccgaggtg aagtcactcg tccggcgcta cctggcccag atgatctccg
     9361 agctcgacga gcagggcgac ccggacagcc ccggccgctc gttgctggag agcatcgagc
     9421 tgatcgtccc gcaccaggcg aacaagacga tgatcgtcga cctcgcccgc aaggcggggc
     9481 tggcggagga acagctgtat ttcaacatcg ccacgatggg caacgtgtcc gccgcgagca
     9541 tcccgatcgc actgcacgac gcggtcggcg acggtgtgat caaacgtccg acgcgggtgt
     9601 tcgcgcccgg tttcggcgcc ggcgccgtcg gtgggtacgc cgtgctcacg atcgacccgg
     9661 cgatcatcgc ccctgaggcc gagccgaaca cggcgccggg gatcgccggg ctgctcacgc
     9721 cgatcgcgac cacgagcgac gacgtccgca tcgcgttcgg cgattgaacc gacggggcgt
     9781 cataaccggg tgttgctcgg ggaacacggt aggtggacgg ctccgagccg agaggccgcg
     9841 ggcaggtgga cgtcgacgag gaaggactcg acatgaccga ccagaaggtg gccatcgtga
     9901 ccgggggcgc gcgcgggatc ggcgccgcga tcaccgattc tttggcccgc tccggcgtac
     9961 acgtcgcggc gggttacagc agcaacagcc gcgcggccga ggagctggcc gggaagctct
    10021 cggcggaggg ctgctccgtg tcggtgcacc agggcaacgt cggcgtgcca gaggactgcc
    10081 agcgggtggt cgaggaggtg ctggccgatc ggggccgcgt cgaccatctg gtcaacaacg
    10141 cgggcgtcac cgtcgacaag accatgcgga agatgacggt ggacgactgg cacgcggtgc
    10201 tccgtaccaa cctgtccggt gccttctaca tgtccaaagc ggtgctcgag cacatgctgg
    10261 aacgcgggtc cgggcgcatc gtcaacatca gctccgtgat cgggcagacg ggcaacgtcg
    10321 gccaggtcaa ctacgcggcc tcgaagtccg ggctgttcgg gatgagccag agcctggcgc
    10381 gggaggtggc ccgcaagggg atcacggtga actgcgtggc acccggctac gtcgagaccg
    10441 agatggtggc cgcggtgccc gagaacgtac tcgagaagat cattgccggc attcccgtgg
    10501 gacgactg
//
LOCUS       DASRSC010000277        11348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2402220,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000277 DASRSC010000000
VERSION     DASRSC010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11348
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2402220"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VFE39_13635"
     CDS             <1..297
                     /locus_tag="VFE39_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6259686.1"
                     /translation="LLLDEPFGALDSLTRTDMQTWLDGVRAHFDWTVLLITHDIREAV
                     FLSDRVVVLGPRPARVGREVIVDLPRPRELGMITTPRFAALEEELLGVLRDRSR"
     gene            complement(365..916)
                     /locus_tag="VFE39_13640"
     CDS             complement(365..916)
                     /locus_tag="VFE39_13640"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6259687.1"
                     /translation="MAWWTRNEGEDGGPDPLAQPGDLHELAARNADGAGLYDAGRPED
                     AVPLFELALAGYRATLGDDDPATLTVAGNLGSACVAAGRWRKGITLITANLADRVRVL
                     GNVDPRTLTARDALATAYRLIGNVDDALALSRLVTADRMRILGPAHPDTLTSRLGMAL
                     VTAAVGDVESALTQLTAALDERA"
     gene            1094..1348
                     /locus_tag="VFE39_13645"
     CDS             1094..1348
                     /locus_tag="VFE39_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259688.1"
                     /translation="MRITRSARKHGIAADDVRRVLDAPVRSVVQGKVELHIGLTANRD
                     LIEVVVVPGEPIIVLHAMRLRPSNYRHLTGPGQHSTGPPS"
     gene            complement(1461..2471)
                     /locus_tag="VFE39_13650"
     CDS             complement(1461..2471)
                     /locus_tag="VFE39_13650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6259689.1"
                     /translation="MKNVAAHAGVSLGTVSNVLNRPEMVSERTRRRVQSSIDALGFVR
                     NDSARQLRGGTSRTLAYVVLDASNPFFTDVAMGVQEGADAAGLAVYLCNSGENSDRQA
                     AYLDLLEQQRVEGVLITPVHAEDPRIAALAARGTPVVLVDRASGPGLCSVTVDDVLGG
                     DLAVTHLLDTGHRRIAFVGGPRAIGQVADRIAGAEQAISRAGAGTLTILDTAALNVAE
                     GRRAGERLAGLPSAHRPTAPFCANDLLALGLLQQMVRLGLRVPDDLAIVGYDDIEFAE
                     AAAVPLTSVSQPRHLLGRTAAELLLSEARGDGAHDHQQLVFAPELVVRASTRLIPVSR
                     PV"
     gene            2721..3545
                     /locus_tag="VFE39_13655"
     CDS             2721..3545
                     /locus_tag="VFE39_13655"
                     /inference="COORDINATES: protein motif:HMM:NF020120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase N-terminal
                     domain-containing protein"
                     /protein_id="HET6259690.1"
                     /translation="MTLPAPTPVRFEHAASGFGIGTPTPRLSWRLPVAPPGFAATGCE
                     LELTNADTGSVTTRRVASAEQVLVPWPFAPLASRARVAVRVRVAAGDDVTPWSAPAPL
                     ETTLLGPADWTAGFISPRTIGRIGDPAPVLRGTVHLGGEIVRARLHVTALGLHVTSIN
                     GQRVGDEELAPGWTSYAHRLSARRSPDSRRGCSAGASGAVLVHATVVLGVLPLRPGAF
                     LLPAVGAVVAGLVAPARRFLAIRGRGRGERDAARGGSCAAPRPGRELSRRVRRRHI"
     gene            3542..4708
                     /gene="rhaI"
                     /locus_tag="VFE39_13660"
     CDS             3542..4708
                     /gene="rhaI"
                     /locus_tag="VFE39_13660"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HET6259691.1"
                     /translation="MNEPAAVHAALREQRIETPSWAYANSGTRFKVFTQPGVPRSPQE
                     KIADAAVVHRLTGAAPSVALHIPWDRVDDYGTLAAYATDLGVALGTINANVFQDDDYK
                     LGSVTNPDPRVRRKAIDHLLECVDIMDATGSRDLKLWFSDGMNYPGQDNIRARQDRLA
                     EALRETYDRLGEDQRLILEYKLFEPAFYTTDVPDWGTSYAHCLELGPRAQVCIDTGHH
                     APGTNIEFIVAFLLRAGKLGAFDFNSRFYADDDLMVGAADPFQLFRILHEIVQGGALD
                     PAMGIAFMLDQCHNIETKIPAIIRSVMNVQEATAKALLVDVAALESAQRSGDVLGANA
                     VLMDAYNTDVRPLLARLRENSGLEPDPIAAYHRSGYQQQIERERVGGRQAGWGA"
     gene            4765..6801
                     /locus_tag="VFE39_13665"
     CDS             4765..6801
                     /locus_tag="VFE39_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HET6259692.1"
                     /translation="MTHPAVQALLARSNRLGADPRNTNYAGGNTSAKDTAEDPVTARP
                     VDLMWVKGSGGDLGTLTEPGLAVLRLDRLRALVDVYPGVEREDEMVAAFDFCAHGRGG
                     AAPSIDTAMHGLVEADHVDHLHPDSGIAIATAADGEALTKEIFGDRVVWVPWRRPGFQ
                     LGLDIAAVKRANPQAVGVILGGHGITAWGATSEECEANSLDIISTAARYLTERGSAEP
                     FGAVRAGYEPLPEAERRARAAALAPVVRGLASTDRRQVGHFTDSDAVLEFLAREKLPA
                     LAALGTSCPDHFLRTKVRPLVLDLPATTPLHEAIARLRELHAAYREDYRAYYERHATR
                     DSPAMRGTDPAIVLVPGVGMFSFGANKQTARVAGEFYVNAINVMRGAEAVSTYAPIPE
                     SEKFRIEYWSLEEAKLARMPKPKPLAGRVAFVTGAGSGIGRAVAQRLAGEGACVVVAD
                     RDGDAAATVAAELGNSDVAVPVTVDVTSTEEVAAAVDAAALAFGGVDLVVNNAGLSIS
                     KPLLETTDDDWDLQHDVMAKGSFLVARAAVRAMIVQRMGGDIVYIASKNGVFAGPNNV
                     AYGAAKADQAHQVRLLAAELGEHGIKVNGVNPDGVVRGSGIFAGGWGAQRAAVYGVPE
                     EKLGEFYAQRTLLKREVLPEHVAAAVFVLVGGDLSHTTGLHVPVDAGVAAAFLR"
     gene            6916..8174
                     /locus_tag="VFE39_13670"
                     /pseudo
     CDS             6916..8174
                     /locus_tag="VFE39_13670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418864.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose/L-arabinose isomerase family protein"
     gene            8183..8521
                     /locus_tag="VFE39_13675"
     CDS             8183..8521
                     /locus_tag="VFE39_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259693.1"
                     /translation="MAAVDLGASSGRVAVVEVGPDRPALTELARFPIGPVRLGGTLHW
                     DVLALYRGVLEGLRAAGPRLGSRVAVTPTRCPRGLVAAWLRALSAVGMPLAGRPRSGG
                     GACSEQCGYD"
     gene            8762..9187
                     /locus_tag="VFE39_13680"
     CDS             8762..9187
                     /locus_tag="VFE39_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259694.1"
                     /translation="MDTFIDRKRKVRIQVVGAGVRRVLVGGRWCAMSSECCAGLVGRS
                     SMWPKSLLNVPIHVARVGPPARRSALAWVRDACAADFLHRGWVAAFVALVVALPVVVT
                     GLASAGGRIGVAGIVVVLVATVAVMTRIDLGSGRTPHHE"
     gene            9253..10026
                     /locus_tag="VFE39_13685"
     CDS             9253..10026
                     /locus_tag="VFE39_13685"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6259695.1"
                     /translation="MEPHRWSWRSWWRPPSARGLLFAAVVLVLTCGALAVVKPAGAAV
                     TASVRDVPGPAVGWSSEPAGLVDPARIGGAAPVLAVVGASFAAGVGAAGPHMGWPYLL
                     ARLEGWRVIVSADPGAGFLNPGAHRLGPLARLVGRLDLARLNPSMVIVQGGYNDVGRS
                     PAAVASQVAALFGSIRRSAPHARLGLVSVFSVGAGPTRTAVALDRTIIAAARKADPAV
                     VVFDPLAERWVFPRIGDDLHPSPAGHAWIAARIEQDLRR"
BASE COUNT         1553 a         3929 c         4098 g         1768 t
ORIGIN      
        1 ctgctgctcg acgagccgtt cggggcactg gactcgctca cccgcacgga catgcagacc
       61 tggctcgacg gcgtacgcgc ccacttcgac tggacggtgc tgctgatcac ccacgacatc
      121 cgagaggccg tgttcctctc cgaccgagtg gtggtgctcg ggccgcggcc cgcgcgcgtc
      181 ggccgcgagg tgatcgtgga tctgccgcgg ccccgcgagc tcggaatgat cacgactccg
      241 cgcttcgccg ccctcgagga ggagttgctc ggcgtattgc gcgaccgttc ccgatgaatg
      301 tcgcacaaag ccggtccggc aagatcagac cgatactcct gtgcggatcg cgaaataagc
      361 cgaatcatgc acgttcgtcc agcgctgccg tgagctgggt gagcgccgac tcgacgtcgc
      421 ccaccgcggc cgtcaccagc gccattccca ggcgggacgt gagcgtgtcg gggtgggcgg
      481 gcccgaggat gcgcatccgg tccgcggtga ccagcctgct cagcgcgagc gcgtcgtcga
      541 cgttgccgat cagccggtag gccgtggcga gggcgtccct ggccgtgaga gtgcgcggat
      601 cgacgttgcc gaggacgcgg acgcggtccg cgaggttcgc ggtgatcagg gtgatcccct
      661 tccgccaccg gccggccgcc acgcacgcgg agccgaggtt gcccgccacg gtgagtgtgg
      721 ccgggtcgtc gtctccgaga gtggcccggt atcccgccag ggccagttcg aagagaggca
      781 ccgcatcctc gggtcgcccg gcgtcgtaga ggcctgcgcc gtcggcgttg cgcgcggcga
      841 gctcgtggag gtcgccgggc tgggcgaggg ggtcgggtcc gccgtcctcg ccctcgttcc
      901 gtgtccacca cgccatcgcg ttcacccccg acggttgctg agcaaattac tgcacgaaag
      961 attgccccgt cagttgtcgg gagagatatc acgtagctct cggggcgctg agccgtcatg
     1021 attcggagaa ctgccggaac tgtcacacgg ggtgggcgat cgatgacgcg gagtcgctcg
     1081 taatatgccg gccgtgcgaa tcacccgtag cgcccgtaaa cacgggatcg cggccgacga
     1141 cgtccgccgc gtgctcgacg cgccggtgcg cagcgtcgtg cagggcaagg tcgagctgca
     1201 catcggactc acggcgaacc gcgacctgat cgaggtggtc gtggtgccgg gcgagccgat
     1261 catcgtgctg catgcgatgc ggctgcgtcc gtcgaactat cggcacctga ccggacccgg
     1321 ccagcactcc accggaccgc cgtcgtagca ccggcactac ctcggccgcg atcacaccgc
     1381 cgtggtgcga agggacgtgt gccggccgtc ggtgcaccgg tggcctgaac cgggcacccc
     1441 cgtcaactgt gcgtcggcga tcagaccggt ctgctcaccg ggatcaaccg ggtcgacgcg
     1501 cggacgacca gctccggagc gaacacgagc tgctggtgat cgtgcgcgcc atcgccgcgc
     1561 gcctcggaca gcaacagctc ggcggcagtg cgtccgagca ggtgccgcgg ctggctgacc
     1621 gaggtcagcg gcacggcagc ggcctcagcg aactcgatgt cgtcgtagcc gacgatggcc
     1681 agatcgtcgg gcacccgcag gccgaggcgc accatctgct gcaggagccc cagtgcgagc
     1741 aaatcgttgg cgcagaaggg ggcggtggga cggtgcgcgc tgggcagtcc ggcgagccgc
     1801 tcgccggcgc ggcggccctc ggcgacgttg agcgcggcgg tgtccagaat ggtcagggtg
     1861 ccggcgccgg cacggctgat cgcctgctcg gcgcccgcga tgcggtcggc aacctgcccg
     1921 atggcacggg ggcctccgac gaacgcgatc ctgcggtggc cggtgtccag caggtgggtg
     1981 accgcgaggt cgccgccgag cacgtcgtcc accgtgaccg agcagagccc gggcccggag
     2041 gcccggtcca cgagcacgac cggggtgcca cgcgcggcga gcgcggcgat ccgcggatcc
     2101 tccgcgtgca ccggcgtgat cagcacgccc tccacccgct gctgctccaa caggtcgagg
     2161 taggcggcct ggcggtcgga gttctcaccg ctgttgcaca ggtacaccgc gaggcctgcg
     2221 gcgtcggcgc cctcctgcac gcccatggct acgtcggtga agaacgggtt cgaggcgtcg
     2281 agcacgacgt acgccagcgt ccggctggtg ccgccacgga gctgacgggc cgagtcgttg
     2341 cgcacgaaac ccagcgcgtc gatcgaggac tgcacgcggc gcctggtgcg ctcgctgacc
     2401 atctcgggcc ggttgaggac gttggagaca gtgccgaggg agacgcccgc gtgcgccgcg
     2461 acattcttca tgctcggtgc gccctgtgcc gccaccaagg tcgccttcct tgttgaaacg
     2521 tggttaagag ggtcgggagc catggcgccg gaccgtgctg gacatcgtac gggcagctga
     2581 gcccgtaaga cgcaggcctt gacctgtcga tcgacggtta cctacagtcg cggccgcgat
     2641 tgaaacgttt tatgggtgtc tccgaacttg gtgtttcgga aggcgtcccg ccgtcccgtc
     2701 ccgcgcttcc tggaggccgc gtgaccctgc cagcccccac ccccgtccgc ttcgagcacg
     2761 ccgcgtccgg cttcgggatc ggcacgccga ccccccgtct gtcctggcgg ctcccggtgg
     2821 cgccccccgg gttcgcggcc accggctgcg agctcgagct caccaacgcc gacaccggca
     2881 gtgtcaccac ccggcgggtc gcctcggccg agcaggtcct cgtcccgtgg ccgttcgccc
     2941 cgctcgcatc tcgtgcccgc gtcgcggttc gggttcgggt ggccgcgggc gacgacgtga
     3001 cgccgtggag cgcgccggcg ccgctggaga cgacgctgct cggccccgcc gactggaccg
     3061 ccggcttcat ctccccgcgc accatcggcc ggatcggcga cccggcaccg gtcctgcgcg
     3121 ggaccgttca cctcggaggt gagatcgtcc gagcccggct tcatgtcacc gcgctgggcc
     3181 tgcacgtcac gtcgatcaac gggcagcggg tcggcgacga ggagctggcg ccgggctgga
     3241 cgtcctacgc ccaccgcctg agcgcacggc gctcgcctga cagccggcgg ggctgttccg
     3301 caggcgcttc cggagccgtg ctcgtccacg cgaccgtcgt gctcggtgtc ctcccgctgc
     3361 ggccgggcgc ctttctcctc ccggcggtgg gtgctgtcgt cgcaggacta gtcgctcctg
     3421 cgcgtcgttt ccttgccatc cgcggtcgag gccgcggaga gcgcgacgca gctcggggcg
     3481 ggagctgtgc cgcgccccgt cctggtcgtg agctctcgcg ccgggtgagg agacgacaca
     3541 tatgaacgaa ccggccgccg tccacgccgc gctgcgcgag cagcgcatcg agaccccgtc
     3601 ctgggcctac gcgaactccg gcacgcggtt caaggtcttc acgcagccgg gcgtgccccg
     3661 ctcgcctcag gagaagatcg ctgacgccgc cgtggtgcac cgcctcaccg gcgccgcacc
     3721 cagcgtggca ctgcacatcc cgtgggaccg ggtggacgac tacggcacgc tggccgcgta
     3781 cgctacggac ctgggggtgg cgctcggcac gatcaacgcc aacgtcttcc aggacgacga
     3841 ctacaagctg ggcagcgtca ccaacccgga cccgcgggtg cgccgcaagg cgatcgacca
     3901 cctgctggag tgcgtcgaca tcatggacgc caccgggtca cgggacctca agctgtggtt
     3961 ctccgacggc atgaactacc ccggccagga caacatccgg gcccggcagg atcggctcgc
     4021 ggaggcgctg cgcgagacct atgaccggct cggcgaggac cagcggctca tcttggagta
     4081 caagctcttc gagcctgcct tctacaccac cgacgtcccg gactggggca cctcgtacgc
     4141 acattgcctc gagctcggcc cgcgcgcaca ggtctgcatc gacaccggcc accacgcccc
     4201 tggcaccaac atcgagttca tcgtggcgtt cctgctgcgg gccgggaagc tcggggcgtt
     4261 cgacttcaac tcgcgcttct acgccgacga cgacctgatg gtgggcgcgg cggacccgtt
     4321 ccagctgttc cggatcctgc acgagatcgt gcagggcgga gcgctcgacc ccgcgatggg
     4381 gatcgccttc atgctcgacc agtgccacaa catcgagacg aagatccccg cgatcatccg
     4441 ctcggtgatg aacgtgcagg aggccacggc caaggcgctg ctcgtcgacg tggcggcgct
     4501 ggagtcggcg cagcgctccg gtgacgtgct cggagccaac gccgtgctga tggacgccta
     4561 caacaccgac gtgcggccgc tgctcgcccg gctgcgcgag aactccggcc tggagcccga
     4621 cccgatcgcc gcctaccacc gctccggcta ccagcagcag atcgagcgcg agcgggtcgg
     4681 cggccgacag gccggctggg gcgcctgacc gccatccgca cactctccgc ccagcacccc
     4741 tcccaccccg ctggagaaca gacgatgacc cacccggcag tccaggcgct cctcgccagg
     4801 agcaaccgcc taggcgccga cccgcgcaac accaactacg cgggtggcaa cacctctgcc
     4861 aaggacacgg ccgaggaccc ggtcaccgcc cggcccgtcg acctgatgtg ggtgaaggga
     4921 tccgggggag acctcggcac cctcaccgag ccggggctcg cggtgctccg gctcgaccgg
     4981 ctgcgcgcac tcgtcgatgt ctatcccggt gtcgagcgcg aggacgagat ggttgccgcc
     5041 ttcgacttct gcgcccacgg caggggcggg gccgcaccgt cgatcgacac cgcgatgcac
     5101 gggctcgtcg aggccgacca cgtcgaccac ctgcaccccg actccgggat cgcgatcgcc
     5161 accgctgccg acggcgaggc gctcacgaag gagatcttcg gcgaccgggt cgtgtgggtg
     5221 ccgtggcggc gccccggctt ccagctcggt ctcgacatcg ccgcggtgaa gcgggcgaac
     5281 ccgcaggccg tcggcgtgat actcggcggg cacggcatca cggcctgggg tgccacgagc
     5341 gaggagtgcg aggccaactc cctcgacatc atctccacgg ccgcccgcta cctcaccgag
     5401 cgcgggtccg ccgagccctt cggcgccgtc cgggccgggt acgagccgct gccggaggcc
     5461 gagcggcgcg cgcgggcggc cgcgctcgcc ccggtggtac gcgggctcgc ctccaccgac
     5521 cgccggcagg tgggccactt caccgacagc gacgcggtgc tggagtttct cgcgcgggag
     5581 aagctcccgg cactggcggc actgggcacc tcctgccccg accatttcct gcgcacgaag
     5641 gtccggccgc tcgtgctgga cctgccggcg acgaccccgc tccacgaggc gatcgcccgc
     5701 ctgcgggagc tgcacgccgc ctaccgcgag gactaccgcg cctactacga gcggcacgcc
     5761 actcgcgaca gcccagcgat gcgcgggacc gaccccgcaa tcgtgctcgt ccccggtgtc
     5821 ggcatgttca gcttcggcgc caacaagcag accgcccgcg ttgcggggga gttctacgtc
     5881 aacgcgatca acgtgatgcg cggcgccgag gccgtgtcga cctacgcccc gatccccgag
     5941 tcggagaagt tccgcatcga gtactggtcc ctcgaggagg ccaagctcgc gcggatgccg
     6001 aagccgaagc cgctcgccgg ccgggtcgcg ttcgtgacag gagcgggttc cgggatcggt
     6061 cgtgccgtcg cccagcggct cgcgggcgag ggcgcgtgcg tggtggtcgc cgaccgcgac
     6121 ggcgatgccg cggcgaccgt ggccgccgag ctggggaaca gcgacgtcgc ggtaccggtg
     6181 acggtcgacg tcacctccac cgaggaggtg gccgccgcgg tcgacgcggc cgcgctggcc
     6241 ttcggcgggg tcgacctcgt ggtcaacaac gccgggctgt cgatctccaa gccgctgctg
     6301 gagacgaccg acgacgactg ggacctccag cacgatgtga tggccaaggg atccttcctc
     6361 gtggccaggg ctgccgtgcg ggcgatgatc gtgcagcgca tgggcggcga catcgtctac
     6421 atcgcgagca agaacggggt gttcgccggc ccgaacaacg tcgcctacgg cgccgccaag
     6481 gccgaccagg cgcaccaggt gcggctgctc gccgccgagc tcggcgagca cgggatcaag
     6541 gtcaacgggg tgaacccgga cggcgtcgtc cgcggctcgg ggatattcgc cggtggctgg
     6601 ggcgcgcagc gcgcggcggt gtacggcgtt ccggaggaga agctcggcga gttctacgcg
     6661 caacggaccc tgctcaagcg cgaggtgctg cccgagcacg tcgccgccgc cgtctttgtg
     6721 ctcgtcggcg gagacctctc tcacaccacc ggcctgcacg ttcccgtcga cgcgggcgtc
     6781 gccgcggctt tcctgcgctg acgcgcccgt gcgcggatac gagtgccgga gcactcgtat
     6841 ccgcgcacga ccgacgaagg aggagatcgc ttggccgcag tactcgaccg ggtcaccccg
     6901 cgccgcacct cggtcggctt catctccgac gcgcaggacg gcgcggtggc cgcggagaag
     6961 ctgcgggtcg ccgggtgcga tctcatcgtc ggcttcctgg ccacctacat gacggcgtcg
     7021 atgctggtcc ccgtcgcgca gcgcagcggc gccccggtgc tgctgatcaa cctgcagccc
     7081 acggagaaga tggaccacgc caccgtcgac accggggctt ggctcgccta ctgcggggcc
     7141 tgcccgctgc cggagatggc caacgcgttc gaccgtgccg gggtggagtt ccggtcggtg
     7201 tcgggctatc tggaggacga gcgcgcgtgg gcgcggatcg cccgctgggt gagggcggcc
     7261 ggcatacgcg gggcgctgcg tcacggccgg cacggactga tggggcagct ctacccgggc
     7321 atgctcgacg tctccaccga tctcacctct gtgtcggcgc aactcggtgg acacgtcgag
     7381 gtgctggaac tcgacgacct gcgggtgcgc gtggaggacg tgaccgaccc caggcgcggg
     7441 aacgggtcga gctgacccgt gaggtcttcg acgtcgacgc ctcggtcaac cccgaggacc
     7501 tcgcctgggg cgcacgcgtc tccgtcggcc tcgaccggct cgtggacgac ttcgcactcg
     7561 actcgctggc ctactaccac cgcggcctcg atggcgagca gcacgagcga ctcggagcgg
     7621 gcctgatcct cggcgcctcg ttgctcaccg cccgcggcgt gccggtcgcg ggggagtacg
     7681 agttgcgcaa ctcgatcgcc atgctcgtca tggaccggct cggcgcggga ggctcgttca
     7741 ccgagctgca ggcgctgaac ttcgccgacg acgtggtgga gatgggccac gacggcccgg
     7801 cgcacctggc gatcagcgcg cgcaggccgc tgctgcgggg gctcggcgtg taccacggca
     7861 agcgtggctg gggcgtctcc gtcgagttcg acgtccaacc cggcccggtc acgctgtgcg
     7921 ggctcgggca gcgccgcgac gggcgctacg tcatggtcgc ctcggagggc gccgtcgtgc
     7981 ccgggccact gctgcagatc ggcaacacca cctcgcgggt cgacttcggc tgcgacccgg
     8041 gggagtggac cgacgcctgg agcgcctcgg gtgtcgacca ccactgggcc ctcggcaccg
     8101 gacaccgggt ggcggagctc gcggccgtcg cggagctcgt ggggctggag ttcgtcaagg
     8161 tgcgcgttcc ctgacggcgc gggtggccgc ggtcgacctg ggtgcgtcca gcgggcgggt
     8221 ggcggtcgtc gaggttggcc ccgaccgacc ggcgctcacc gagctcgcgc ggttcccgat
     8281 cggtcccgtc cgcctgggcg ggaccctcca ctgggacgtg ctggccctct accggggcgt
     8341 gctcgagggc ctccgcgccg ccgggccacg gctcggttcg cgcgtcgcgg tgactcccac
     8401 tcggtgtcca cgcggcctcg tcgccgcctg gctgagggca ctgtcggcgg tggggatgcc
     8461 gctggctggc agaccacgga gcggaggcgg ggcctgcagc gagcagtgcg gctacgacta
     8521 gggccagtgc ggcccgtcgg cgtcctgagc ggcgtcgatc gagaatcacg ggcgaggtgc
     8581 cgaccggctc gtcccgttcc ggtagggcgg tcactgatgg gcttcctgtg ccggaaaggt
     8641 cagatgggtg ttcatcggag ccttgctctc acggcctggt atcgaggctg atgccgtgaa
     8701 tgcgaaccat tagtcatcac ctaaccgaac agtgggcgtg ttctaacctg ctgtgactgc
     8761 ggtggacacg ttcatcgaca ggaaaaggaa agtaaggatc caggtggtcg gcgcgggtgt
     8821 tcggcgggtt ttggtgggtg gccggtggtg cgcgatgtcg tcggagtgct gcgcgggctt
     8881 ggtcggccgt tcctcaatgt ggccgaaatc gctgctgaat gttccgatcc atgtggcccg
     8941 cgtcggcccg ccggcgcggc gatctgcgtt agcatgggtg cgtgatgcct gtgcagcgga
     9001 ctttcttcac cgaggatggg tcgccgcatt cgtcgcgttg gtggtcgcgc tccccgtcgt
     9061 cgttacgggc ctggcttcgg ccgggggccg gatcggcgtc gcaggcattg tcgtcgtcct
     9121 cgtggccact gtcgcggtga tgacgcgcat cgatctcggg tcgggccgca caccccatca
     9181 cgagtagaca tctcgtcagc tgtcccgatg cgccgccgcg accccgctcg gctctccacg
     9241 aggagcacga tcatggagcc ccaccgctgg tcctggcgat cgtggtggcg gccgccatcg
     9301 gctcgcggtc tcctgttcgc cgccgtggtc ctggtcctga cctgcggggc gctggccgtg
     9361 gtgaaaccgg ccggtgcggc cgtcacggcg tcggtccggg acgttccggg tcctgcagtg
     9421 ggttggtcgt ccgagccggc aggtctggtc gatccggcgc gcatcggtgg ggccgcaccg
     9481 gtgctggcgg tggtcggcgc atcgttcgct gcgggcgtgg gtgcggccgg gccgcacatg
     9541 gggtggccgt atctgctggc acggctcgag ggatggcgag tgatcgtcag cgcggacccg
     9601 ggtgccgggt tcctcaatcc cggagcacat cgcctcgggc cgctcgcccg actcgtcggc
     9661 cgtctcgatc tggcccggct gaacccctcg atggtgatcg tccagggtgg ctacaacgac
     9721 gtcggccgtt caccggccgc cgtcgccagc caggtggcgg cgctgttcgg ctccatccgt
     9781 cgatcggcgc ctcatgctcg actgggcctg gtgtcggtct tcagcgttgg cgcaggtccg
     9841 acgcgcaccg cggtcgctct ggatcgcacc atcatcgccg ccgctcggaa agccgatccc
     9901 gcagtggtcg tgttcgatcc gctcgctgag cggtgggtct ttccccgcat cggtgacgac
     9961 ctgcatccct ccccggccgg gcacgcctgg atcgccgcgc gtatcgagca ggatctgcgc
    10021 cgctgaccgt cgctgatcga cgcgagcgcc tactcgcaga tcggtggtgt gctggtgcgg
    10081 agcgtggtgt tccacgaaac gagcgtggac agtgagagtg cggcgcagcc ggccagagct
    10141 cgacgctgcc gcagtggtgg gtcaccacga tcgcgatggc ggtgcagtcg ttgcccgtcc
    10201 ccggcccgta ggtctggaag gccgcgctga cctggccggg gtgcgtggtg gcgagggcgt
    10261 tgatgctggt gagaagggcg cggcggcgtc gtcggtcgcg ctgtcctcca ggccgtggtt
    10321 gcccggcggg caggtcgggc cgccgtgcgt cgccgaggcg acgcagttgg tcatgatccc
    10381 cttcggggat cgtgctgtcg accgcgatct tccccgacgt tggctgcgga acgggttgac
    10441 ggtgtagtcg accggggtgc gctgccaccc gcagtggtgc tgcccgccgc ccgaaccctt
    10501 ggttgccggc gcaggagcgc ggcgaggacg gtgcgggctg ctacctccac tatgaagtcc
    10561 gcgcaagtcc tcacgccgct ggttcgcttc accggatggt ccacgagaac aatgacaagc
    10621 aacgaatagc aacaccaaag tctgccctct cgacaatttc gtcacgcacc tatgcattag
    10681 ataacatctt ggtacggtgg catttatgct ccggatcacc gccgtatccg tcggggcggt
    10741 ggatcacctg atccgggcag cggctgcacc gaccacgacc acgaccacgg gcccgaccgc
    10801 tcggacggcc gcgagcgtgg cgcggtggcc ggccacgacg gtgcggccgc cagcgtgggg
    10861 cacggtggtc ggggtggggc ggtgggtgac gcggccgggt aatgggtgcg gcagtgcggt
    10921 cgggggaggc gccgggtcgc tggatgggtt ccggcctagc catggtgggg gtggatcggt
    10981 cgcgccggcg ggggaggtgg cggtgtccgc tggtgctggc ggggtagtag gggcgacgca
    11041 ttcggtgttc ggtggcgcgt tcatcgcggg ggcgagggcc accaccgggc accccgtgat
    11101 cgatgagctg gtcggtttcg cgagttcaca ccgacttgtt caatgcgggc aggagatcgg
    11161 gtctgcagcc atgatcgccg aggcggtggt cgggtcgctc ggttcacacc tgtcgcccat
    11221 ccggggctga ctgttcttcg gcctgtgtcc cggccatcgc cgtcgaggtg agtggctgga
    11281 gaggcctgct tcgcccgaag tcggtgtgcc gtctcctcag gcccgtgctg gtcctcggcg
    11341 aggaggtt
//
LOCUS       DASRSC010000278         9951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4040974,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000278 DASRSC010000000
VERSION     DASRSC010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4040974"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /gene="kdpB"
                     /locus_tag="VFE39_13690"
     CDS             <1..1589
                     /gene="kdpB"
                     /locus_tag="VFE39_13690"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HET6259696.1"
                     /translation="TGESAPVIRESGGDRSSVTGGTTVLSDRVVVKIMTKPGESFVDR
                     MIALVEGASRQKTPNEIALTILLAVLTIIFLLAVVAVAPMSTYAGVNQSLIVLTALLV
                     CLIPTTIGALLSAIGIAGMDRLVQRNVLATSGRAVEAAGDIDTLLLDKTGTITFGNRQ
                     ATELIPVSGVSVRDLAVAARLSSLADQTPEGRSIITLCAREHGLPGEATTAEAVAEFV
                     AFTAQTRMSGVDIGGRSVRKGAASAVAKWTAKSDHQVDAVVDEISDGGGTPLVVAEGG
                     SPDGTGARVLGVIRLSDVVKPGMAARFAELRAMGIRTVMITGDNPRTARAIAAEAGVD
                     DHLAEATPEDKMALIKKEQEGGRLVAMTGDGTNDAPALAQADVGVAMNTGTAAAKEAG
                     NMVDLDSDPTKLIEIVEIGKQLLITRGALTTFSIANDLAKYFAILPAMFVAIYPALGA
                     LNIMHLATPQSAILSAVIFNALIIVALIPLALRGVQYRPTSAANLLRRNLLIYGLGGV
                     IVPFVGIFVIDLLVRLIPGI"
     gene            1593..2210
                     /locus_tag="VFE39_13695"
     CDS             1593..2210
                     /locus_tag="VFE39_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HET6259697.1"
                     /translation="MLTMLWRQTATGLRVLIAFTILCGIVYPLVVYGVGQIPGLAAKS
                     AGSIVYAANGTPTGSSLIGVDPVAANPAADPYFHTRPSASSSGPLGPADTSTSGGSNL
                     SGFNQHLLDLVNQRRELIAQREGVNPALVPPDAVTASGSGLDPDISPAYAGLQVPRVA
                     RVTGLPEDTVRTLVAHATSGRILGFLGEPTVNVTTLNQELQEAQH"
     gene            2322..4853
                     /locus_tag="VFE39_13700"
     CDS             2322..4853
                     /locus_tag="VFE39_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HET6259698.1"
                     /translation="MSRRGELRIHLGAAPGVGKTYAMLGEARRRAERGTDVVVGIVET
                     HGRSKTADLLEGLEVVSRRGVDGYPGLDELDLDAVLARKPEVVLIDELAHTNAPGSRN
                     RKRWQDVEELLEAGSDVLTTVNVQHLESLNDVVQRITGVRQQETVPDEVVRRAEQIEL
                     IDITPEALRRRMAHGNVYAAEKVDAALANYFRPGNLIALRELALLWVADEVDVALQRY
                     RTEKEISEVWETRERVVVALTGDRESETVLRRAARIAKRAGAADLLAVHVLRGDGLAG
                     PPVGAVARLRSLAEDVGASFHTVVGGEDVPAALLDFARGANATQLVIGTSRRSRLARV
                     FVEGIGARVVQDSGSIDVHMVTHDEAGRGLRLPALHSGLSGTRRVLGWVLAAAMPLAA
                     TGVGIVLRGLVGMSTDVVLFFLATVIVAMVGGLGPAVVAAVVGGALLNFFLTPPLYSL
                     TIGTAENLLAIAAMVIVAVLVAIVVDRAARRAQQAARSRAEGALLASFARTVLTRRDP
                     LPRLLEKVREAFGLHSVALLERTGGTASDHWSCVSSSGTVYCMSPEEADVDVEIEDDL
                     HLIGSGRSLPASDRRLLEVVGGQALLALRSQRAAAEADDSRRRAHATETRSALLSTVG
                     HDLRSPLTSIKAAAGSLRDPNLQLPPADRQELMATIEESADRLTGLVDNLLDSARLAS
                     GTVTPLLAPIGYDEVAVRALVGLPNADKVTVDIDESLPDVVADAGLLERVVANVVENA
                     LRYAAGASIVLRASAYADRVELRIVDSGPGVPKHGRDALFAPFQRLGAAVPSDRDGAT
                     GVGLGLSVARGLTEAMDGSLTAEDTPGGGLTVVISMPAWSNGAGR"
     gene            4850..5539
                     /locus_tag="VFE39_13705"
     CDS             4850..5539
                     /locus_tag="VFE39_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6259699.1"
                     /translation="MTEHRVLVVDDDLQILRALRINLTAHGYHVLLAPDGGAALRAAA
                     DGHPDVVVLDLGLPDMDGIEVIEGLRGWSTVPIIVLSARTGGPETVRALDAGADDYVT
                     KPFGMAELLARLRAAVRRAAVSAVDGEPIVDAGDFSVDLAAKKVVRADGNELHLTPTE
                     WGVLELLVRHRGKLVGQRELLRAVWGPTYGTETNYLRVYMAQLRRKLEPEPSRPKHLI
                     TEPGMGYRFEG"
     gene            complement(5562..6263)
                     /locus_tag="VFE39_13710"
     CDS             complement(5562..6263)
                     /locus_tag="VFE39_13710"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HET6259700.1"
                     /translation="MYRPRHRGGILLLGITTGVLLIGSVALTAPPGIVPTDWHAALGV
                     AAVAAAPAVVPMPRRAAAPLEVAIAPPPLPVVAPQATPAPVALPTTSSSAPARAVSAP
                     MTKREVLRAASPVYDVVTATNARRSSAGCPALKLDPRLTAAAQQHASDMAAGGYFSHT
                     SQDGTTFDARIRAEGYGSPGGENIARGQQSEAEVVDDWMASPGHRRNIVDCSFTAIGV
                     GYAGKGDYWVQDFGR"
     gene            6542..>9951
                     /gene="smc"
                     /locus_tag="VFE39_13715"
     CDS             6542..>9951
                     /gene="smc"
                     /locus_tag="VFE39_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET6259701.1"
                     /translation="MHLKSLTLKGFKSFASATTLRLEPGITCVVGPNGSGKSNVVDAI
                     SWVLGEQGAKALRGGKMEDVIFAGTSGRAPLGRAEVTLTIDNSDGALPIDYSEVSITR
                     RMFRDGAGEYEINGSHCRLLDIQELLSDSGIGREMHVIVGQGQLDSVLQSKPEDRRAY
                     IEEAAGVLKHRKRKEKALRKLDAMQANLTRLTDLTAELRRQLKPLGRQAEIARRAQAV
                     QSELRDSRLRLAADDLVTLRDALAREEADESTARARRAEVEEQLSVARVEQERLELAL
                     AQDAPRLAAAQDTWYRLSALAERLSGTVRLAQERTRHLADPQERTPGRDPDELDAEAD
                     AVSEQEDELVAAVSEARRRLSDVLEERAEHEKILAAAERAHMAAVRALADRRAGLATL
                     AGRAEALRSGAAATADEIERLSEALAEAQERTETAEAELARAGDELGALDDGDTGLEQ
                     RCAEAVDAHESARKRVAELVARERELEQQRAHWRARVDALSVGLARRDGSGALLGTDG
                     VLGAVSGLLTVETWARVAVAAALGELADAVAVASPDAAVDALRLLRARDAGRAALVVG
                     SAEQESDRPDPGPAPVGRWAVEGVAAGGALAGAVHRLLAGVVVVDDLAAARAVLESDP
                     GLIAVTVDGDVLGAHRASGGSGAATSALDVQAAVNEAVEQREAVEQELVRLRPALDGA
                     RAEEAARLADVEAAQRVQAAAEQRRSAASAQLGRYEQAVRSATAEAQRLRQRRDDVEA
                     RRSDALLALEAAEQQLAVAEDEPVDDEPDTEIRDAAAESLAAARAEEVEARLTLRTVE
                     ERARAIAGRADSLRRQASSERQARARAAAAREARERGASIAALVARAGETACERIAVS
                     LAAAAAERDAAAAARDSRERALGEARATSTRLTSLMEKLTDAVHRDEVLRAQSRLRLE
                     QLTEKVLADHGLGIDDLVAEYGPDAPVPASAAEMAEYEAAKERGEDVVPPPPMPYDRA
                     AQQRRAQRAEKDLGLLGKVNPLALEEFAALEERYRFLSTQLEDLKNTRRDLLTVVREV
                     DDKILEVFAIAYADVAREFEIVFATLFPGGEGRLILTDPDDMLTTGIDVEARPAGKKV
                     KRLSLLSGGERSLVAVAFLVALFKARPSPFYILDEVEAALDDTNLGRLL"
BASE COUNT         1387 a         3485 c         3706 g         1373 t
ORIGIN      
        1 tcaccggcga gtcggccccg gtgatccgcg agtccggtgg tgaccgcagc tcggtcaccg
       61 gcggcaccac ggtgttgtcg gaccgggtcg tcgtgaagat catgacgaag ccgggcgagt
      121 cgttcgtcga ccggatgatc gcgctggtcg agggtgcctc gcggcaaaag acgcccaacg
      181 agatcgcatt gacgatcctg ctggcggtgc tgacgatcat cttcctgctg gccgtggtgg
      241 cggtggcgcc gatgagcacc tacgcggggg tgaaccagtc gctgatcgtc ctgaccgccc
      301 tgctcgtctg cctgatcccg acgacgatcg gtgcgctgct gtcggcgatc ggtatcgcgg
      361 ggatggaccg gctggtgcag cgcaacgtgc tggccacgtc gggtcgcgcg gtcgaggccg
      421 ccggtgacat cgacacgttg ctgctggaca agaccggcac gatcaccttc ggcaaccggc
      481 aggcgacgga gctgatcccg gtctccgggg tcagcgtgcg ggaccttgcc gtggcggccc
      541 ggctctcgag cctggccgac cagaccccgg aaggccggag catcatcacg ctctgcgctc
      601 gggagcacgg gttgccgggc gaggccacga cggccgaggc cgtcgccgag ttcgtggcgt
      661 tcaccgcgca gacccgtatg tcgggtgtcg acatcggcgg ccggtcggtg cgcaagggtg
      721 cagcgtcggc ggttgcgaag tggacggcga agagtgacca ccaggtcgat gcggtggtcg
      781 acgagatctc cgatgggggc ggtacaccgc tcgtcgtcgc cgaagggggc tccccggacg
      841 gcaccggtgc ccgtgtgctg ggcgtgatcc ggctgtcgga cgtggtgaag cccggtatgg
      901 ccgcacgctt cgccgagttg cgggcgatgg gcatccgcac ggtgatgatc accggcgaca
      961 acccgcgcac cgcgcgggcg atcgccgccg aggcgggggt ggatgaccac ctcgcggagg
     1021 cgacgcccga ggacaagatg gcgttgatca agaaggagca ggagggcggt cggctggtcg
     1081 cgatgaccgg cgacggcacc aacgacgcgc ctgcgctggc gcaggccgac gtcggggtcg
     1141 cgatgaacac ggggaccgcg gcggccaagg aggccggcaa catggtcgac ctcgactcgg
     1201 acccgaccaa gctgatcgag atcgtcgaga tcggcaagca gttgctgatc acccgcggcg
     1261 cgctgaccac gttcagcatc gccaacgacc tggcgaagta cttcgcgatc ctgccggcga
     1321 tgttcgtggc gatctacccg gcattgggtg cgctgaacat catgcatctg gcgacaccgc
     1381 agtcggcaat cctgtcggcg gtcatcttca acgcgttgat catcgtggcg ctgatcccgc
     1441 tggctttgcg cggtgtgcag taccggccca cgagcgcggc gaacctgttg cgccgcaact
     1501 tgttgatcta cgggctgggt ggcgtgatcg tgccgttcgt cgggatcttc gtcatcgacc
     1561 tgctcgtccg tctgattccg gggatctgag acatgctcac catgctgtgg cggcagaccg
     1621 ccaccggtct gcgcgtgctg atcgcgttca ccatcctctg cgggatcgtc tacccgctgg
     1681 tcgtgtacgg ggtggggcag atccccgggc tggcggccaa gtcggcgggc tcgatcgtct
     1741 acgccgccaa cggcacgccc accgggtcgt cgttgatcgg ggtcgacccg gtcgcggcca
     1801 accccgccgc cgacccctac ttccacaccc ggccatcagc ctcgtcgtcc ggcccgctgg
     1861 gcccggccga caccagcacc tcgggtggct ccaacttgag cgggttcaac cagcacctgc
     1921 tcgacctcgt caaccagcgg cgtgagctca tcgcccagcg ggagggcgtc aacccggcgc
     1981 tggtgccgcc cgacgcggtg accgcatccg gctccgggct cgacccggac atcagcccgg
     2041 cgtacgccgg gctgcaggtg ccccgtgtcg cgcgcgtgac cgggctcccc gaggacacgg
     2101 ttcgcaccct cgtcgcccac gcgacgtccg ggcgcatcct gggcttcctc ggcgagccga
     2161 cggtcaacgt caccacgctg aaccaggagc tgcaggaggc ccagcactga ccgctccgcc
     2221 agccggtgcg ccggggatcg tcgtcacggc gatccccggc gcgcacggct gctcactccg
     2281 ccccggctcc tgcaagcggc agccggagca gaatcagtga cgtgagcagg cgcggagagc
     2341 tgcggatcca cctcggggcg gcgcctgggg tcgggaagac ctacgccatg ctcggtgagg
     2401 cccgacgccg ggcggagcgg ggcaccgatg tcgtcgtcgg gatcgtcgag acgcacggcc
     2461 ggtcgaagac cgccgacctg ctggagggcc tcgaagtcgt ctcccggcgt ggcgtcgacg
     2521 gctacccagg ccttgacgag ctcgacctcg acgccgtcct cgcgcgcaag cccgaggtcg
     2581 tgttgatcga cgagctcgcc cacaccaacg cacccggctc gcgcaaccgc aagcgctggc
     2641 aggacgtcga ggagctgctc gaggccggca gcgacgtgct gaccacggtg aacgtccagc
     2701 acctcgagtc gctcaacgac gtcgtccagc ggatcaccgg cgttcgccag caggagaccg
     2761 tgcccgacga ggtggtgcgc cgggccgagc agatcgaact gatcgacatc acgccggaag
     2821 ccctgcgccg acgcatggcc cacggcaacg tctacgccgc ggagaaggtc gatgcggcgc
     2881 tggcgaacta cttcaggccg ggcaacctca ttgcgctgcg cgagctggcc ctgctctggg
     2941 tggcggacga ggtggacgtg gcgctgcagc gttaccgcac cgagaaggag atcagcgagg
     3001 tctgggagac ccgcgagcgg gtggtcgtcg ccctgaccgg ggaccgggag agcgagacgg
     3061 tgctgcgccg ggccgcccgg atcgccaagc gcgccggcgc cgccgacctg ctggccgtgc
     3121 acgtgctgcg cggcgacggg ctcgcggggc cgccggtcgg agccgtggcc cgactgcgct
     3181 cgctggcgga ggacgtcggc gcgagcttcc acacggtggt cggcggggag gacgtgcctg
     3241 ccgccctgct cgacttcgcc cgcggggcca acgccacgca gctcgtgatc ggcacgtccc
     3301 gccggtcccg gctggcgcgg gtgttcgtcg aggggatcgg ggcccgtgtg gtgcaggact
     3361 ccgggtcgat cgacgtgcac atggtcaccc acgacgaggc cggtcgtgga ttgcggctcc
     3421 cggcgctgca cagcgggctg tcgggcaccc gcagggtgct gggctgggtg ctcgcggcgg
     3481 cgatgccgct cgcagccacc ggtgtgggca tcgtgctgcg cggcctcgtc ggcatgtcca
     3541 ccgatgtggt gctgttcttc ctcgccacgg tcatcgtcgc gatggtgggc ggcctcggtc
     3601 ccgcggtggt ggccgccgtg gtcggcggcg cactgctgaa cttcttcctg accccaccgc
     3661 tctactcgtt gaccatcggc acggcggaga acctgctggc gattgcagcg atggtgatcg
     3721 tggcagtgct cgtcgccatc gtcgtcgacc gcgcggcgcg gcgcgcccag caggctgcac
     3781 ggtctcgggc cgaaggggcg ctgctggcgt cgttcgcccg cacggtgctc acccggcgcg
     3841 atccgctgcc ccgtctgctg gagaaggtgc gcgaggcgtt cggcctgcat tctgtcgcgt
     3901 tgctggagcg gacgggtggc acggcatcgg atcactggtc ctgcgtctcc tcgtcgggga
     3961 cggtgtactg catgtcgccg gaggaggccg acgtcgacgt cgagatcgag gacgacctgc
     4021 acctcatcgg cagtggccgt tccttgcccg cctcggaccg gcgcctgctg gaggttgtgg
     4081 gcgggcaggc gttgctcgcg ctgcgcagcc agcgggccgc ggcggaggcc gacgactcgc
     4141 ggcgccgggc ccacgccacc gaaacccgca gcgcgttgct gtcgaccgtc ggccatgacc
     4201 tgcgcagccc gctgacctcg atcaaggctg cggccggcag cctgcgggac ccgaacctcc
     4261 agctgccgcc ggcggaccgc caggagctga tggcgaccat cgaggagtcg gccgaccggc
     4321 tcacggggct cgtggacaac ctgctcgact ccgcccgcct cgcctccggc accgtcacgc
     4381 cgctgctggc gccgatcggg tacgacgagg tcgccgtcag ggcgctcgtc ggcttgccga
     4441 acgccgacaa ggtcacggtg gacatcgacg agtcgcttcc ggacgtggtc gcggacgccg
     4501 gcctgctgga gcgcgtcgtc gcgaacgtcg tcgagaacgc tctgcgctat gcggccgggg
     4561 cctcgatcgt gctgcgggcg agtgcctacg ccgaccgcgt cgagctgcgg atcgtcgact
     4621 ccggcccggg ggtgcccaag cacgggcggg acgcgctgtt cgccccgttc caacggctcg
     4681 gcgccgcggt gcccagtgac cgcgacggcg cgacgggcgt ggggctcggg ttgtcggtcg
     4741 cgcgcgggct caccgaggcc atggacggct ccctcaccgc ggaggacacg cccggcggcg
     4801 gcctgaccgt ggtcatctca atgcccgcgt ggtcgaacgg ggcaggccga tgaccgagca
     4861 ccgcgtgctg gtggtcgacg acgacctgca gatcctgaga gcgctgcgga tcaacctcac
     4921 cgcgcacggc taccacgtcc tgctggcccc cgacggcggc gccgcgctgc gcgccgccgc
     4981 cgacgggcac cccgacgtcg tcgtcctcga cctcgggctg cccgacatgg acggcatcga
     5041 ggtcatcgag gggctgcgcg gctggagcac cgtcccgatc atcgtgctgt cggcccggac
     5101 cggcggaccc gagaccgtcc gcgccctcga cgccggcgcc gacgactacg tcaccaaacc
     5161 cttcgggatg gccgagctgc tggcgcgact gcgggccgcg gtccgtcgtg ccgcggtctc
     5221 cgccgtggac ggcgagccga tcgtcgacgc cggggacttc agcgtcgacc tcgcggccaa
     5281 gaaggtggtg cgcgccgacg gcaacgagct gcacctcacc cccacggagt ggggcgtgct
     5341 ggaactcctg gtacgtcacc gcggcaagct cgtcgggcag cgcgagctgc tccgggcggt
     5401 gtggggcccg acctacggca ccgagaccaa ctacctgcgg gtgtacatgg cgcagctgcg
     5461 ccgcaagctg gaaccggagc cctcccggcc caagcacctg atcacggagc cgggaatggg
     5521 ctaccgcttc gaagggtgat cagcagggtg tggagcggtg gtcagcggcc gaaatcctgc
     5581 acccagtagt cgcccttgcc ggcgtagccc acgccgatcg cggtgaacga gcagtccacg
     5641 atgttgcgcc tgtgaccggg ggaggccatc cagtcgtcca cgacctcggc ctcgctctgc
     5701 tggccccgcg cgatgttctc cccgccgggg gaaccgtagc cctccgcgcg gatcctggcg
     5761 tcgaacgtcg tgccgtcctg gctggtgtgg gaaaagtagc caccggcggc catgtccgag
     5821 gcgtgctgct gggcggcggc ggtgagcctg gggtcgagct tcagcgcggg gcagcccgcc
     5881 gagctccgcc tggcgttggt ggcggtgacg acgtcgtaga cggggctggc ggcccggagg
     5941 acctcgcgct tggtcatcgg agccgagacg gctcttgccg gcgcggacga gctggtggtc
     6001 ggtagggcga cgggggcggg cgtcgcctgc ggggcgacga cggggagggg cggcggcgcg
     6061 atcgccacct cgagtggcgc ggctgcacgc cggggcatcg ggacgaccgc cggggccgcc
     6121 gcgaccgctg ccactccgag cgcggcatgc cagtctgtcg gcacgatccc ggggggagcc
     6181 gtgagagcca cgctaccgat caagagcacc ccggtcgtga tgccgaggag caggatcccg
     6241 ccacgatgcc tgggcctgta cacggccgta acgtagcagg accattggga cgtaccggtt
     6301 ccgccgttcg gctgaatgtc gggtatttca ggtcaagttc agcccatggc tctccgaaca
     6361 tagctttgtg tagtccgcgc gagcccgacc tgtcggtagg gcccgggatc ggcaggcgtt
     6421 ccagcggcgg cgggaggcac tcccgggcga gcggtgctgc gacgccggcg gcggcctaca
     6481 gggattcgtc gcagcctgac ggtaatctgc gtcgaccgcc gcccccctgg agaacccact
     6541 cgtgcatctc aagagcctga cgctgaaggg cttcaagtcc ttcgcctcgg ccacgacact
     6601 gcgcctggag ccggggatca cctgcgtggt cggcccgaac ggctcgggca agtccaacgt
     6661 cgtcgacgcg atcagctggg tcctcggcga gcagggagcg aaggctctcc gtggcgggaa
     6721 gatggaggac gtcatcttcg ccggcacgtc gggacgcgct ccgttgggcc gcgccgaggt
     6781 gacgctgacg atcgacaact ccgatggggc gttgccgatc gactactccg aggtgtcgat
     6841 cacccgccgg atgttccgcg acggcgcggg cgagtacgag atcaacgggt cgcactgccg
     6901 gttgctcgac atccaggagc tgctgtcgga ctccggcatc ggccgcgaga tgcacgtgat
     6961 cgtcgggcag ggccagctcg actcggtgct gcagtccaag ccagaggacc gcagggccta
     7021 catcgaggag gccgcgggcg tcctcaagca tcgcaagcgc aaggagaagg cactccgcaa
     7081 gctcgacgcg atgcaggcca acctcacccg actcaccgac ctcaccgcgg agctgcgccg
     7141 ccagctcaaa ccgctggggc ggcaggcgga gatcgcccgc cgcgcgcagg ccgtccagtc
     7201 cgagctgcgg gactcccggc tccggctcgc cgccgatgat ctggtcactc tgcgtgacgc
     7261 gctggcccgc gaggaggccg acgagtcgac ggcccgcgcc cgccgggccg aggtggagga
     7321 gcagctgtct gtcgcgcgcg tcgagcagga acggctggag ctcgctttgg cacaggacgc
     7381 cccgcggctg gccgccgcgc aggacacctg gtaccgcctc tccgcgttgg ccgagcgcct
     7441 ctccggaacc gtccggcttg cgcaggagcg cacccgccac ctcgccgacc cgcaggagcg
     7501 caccccgggg cgggaccccg acgagctgga cgcggaggcc gacgccgtct ccgaacagga
     7561 ggacgaactg gtcgcggccg tctccgaggc gcgcaggcgg ctgtcggacg tgctggagga
     7621 acgcgccgag cacgagaaga tactggccgc cgccgagcgt gcccacatgg ccgccgtccg
     7681 cgcgctggcc gaccgccggg ccgggctcgc cacgctcgcc gggcgcgccg aggccctgcg
     7741 cagcggtgcc gccgccaccg ccgacgagat cgaacggctg tccgaggcgc tcgccgaagc
     7801 acaggagcgc accgagaccg cggaggccga gctggccagg gccggcgacg agctcggcgc
     7861 cctcgacgac ggcgacacgg ggctcgaaca gcgctgcgcc gaggccgtcg acgcgcacga
     7921 gagcgcccgc aagcgagtcg ccgagctggt ggcgcgcgag cgcgagctcg agcagcagcg
     7981 tgcgcactgg cgggccaggg tcgacgcgct gtcggtcggg ctggcccggc gcgacggttc
     8041 cggcgctctg ctgggcaccg atggtgtgct cggcgcggtg tccggcctgc tcaccgtcga
     8101 gacatgggcc cgcgtggccg tcgcggccgc gctgggtgag ctcgccgacg cggtggcggt
     8161 ggcctcgccc gacgctgccg tggacgcgct gcgcctgctc cgcgcccgcg atgccggccg
     8221 ggcggcgctg gtggtcggct ccgcagagca ggagagcgac cgtcccgacc ccgggccggc
     8281 gcccgtcggg cgctgggcgg tggagggtgt cgccgccggc ggcgcgctcg ccggcgcggt
     8341 gcaccgcctg ctggccggcg tcgtcgtcgt cgacgatctg gccgcggccc gggcggtgct
     8401 cgagtccgac cccgggctga tcgccgtcac cgtcgacggc gacgtcctgg gtgcacatcg
     8461 cgcctcaggc ggctccggtg cggccaccag cgccctggac gtgcaggctg cggtcaacga
     8521 ggccgtcgag cagcgtgagg ccgtcgagca ggagctggtg cggctgcgtc cagcgctcga
     8581 cggcgcccgc gcggaggagg ccgcccggct ggcggacgtc gaggccgcgc aacgggtgca
     8641 ggcggccgcc gagcagcggc gctccgcggc gtcggcgcag ctggggcgct acgagcaggc
     8701 cgtgcggtcg gccaccgcgg aggcccagcg gctgcggcag cgacgcgacg acgtggaggc
     8761 ccgccgctcc gacgccctcc tggcgctgga ggccgccgag cagcagctcg cggtggccga
     8821 ggacgaaccg gtagacgacg agcccgacac cgagatccgg gacgccgccg ccgagtcgct
     8881 cgccgccgcg cgcgccgagg aggtggaggc gcggctcact ctacgcacgg tcgaggagcg
     8941 ggcccgggcc atcgccgggc gcgccgactc cctgcgtcgg caggcgtcgt cggagcggca
     9001 ggccagggcc agggcggctg ccgctcgtga ggcgagggag cggggcgcgt cgatcgcagc
     9061 gctcgtcgcc cgcgcaggcg agacggcgtg cgagcggatc gccgtgtcgc tggccgcggc
     9121 cgccgccgag cgcgacgccg ctgcggcggc ccgcgactcc cgcgagcgcg cgctcggcga
     9181 ggccagggcc acttcgacgc gcctgacctc gctcatggag aagctcaccg acgccgtgca
     9241 ccgcgacgag gtgctgcgcg cgcagtcccg gctgcggttg gagcagctca ccgagaaggt
     9301 gctcgccgac cacggcctcg gcatcgacga cctcgtcgcc gagtacggcc ccgacgcgcc
     9361 ggtgccggca tcggccgcgg agatggccga gtacgaggcg gcgaaggagc gcggcgagga
     9421 cgtcgtgcca cccccaccga tgccctacga ccgcgctgcc cagcagcggc gggcgcagcg
     9481 ggcggagaag gacctcggac tgctgggcaa ggtcaacccg ctcgcgttgg aggagttcgc
     9541 ggcgctcgag gagcgctacc ggttcctgtc cacgcagctg gaggacctga agaacacccg
     9601 tcgcgacctg ctcaccgtcg tccgggaggt cgacgacaag atcctcgagg tgttcgccat
     9661 cgcgtacgcc gacgtggcac gggagttcga gatcgttttc gccacgctgt tccccggcgg
     9721 cgaagggcgg ctgatcctca ccgatcccga cgacatgctc accacgggca tcgacgtcga
     9781 ggcgcgaccg gcaggcaaga aggtcaagcg actgtcgctg ctttccggtg gagagcgctc
     9841 gctggtcgcg gtggcgttcc tggtcgcgct gttcaaggcc cggccgtcgc cgttctacat
     9901 cctcgacgag gtcgaggccg cgctcgacga caccaacctc ggccggttgc t
//
LOCUS       DASRSC010000279        14747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5021383,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000279 DASRSC010000000
VERSION     DASRSC010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 14747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14747
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5021383"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VFE39_13720"
     CDS             complement(<1..212)
                     /locus_tag="VFE39_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259702.1"
                     /translation="MVGRCRHTTVRSSASSPTRSAIAATRSPADLRLDGDKVVDTSGE
                     VRVALAEVLGGDVVEETVEFRHRPTEP"
     gene            139..681
                     /locus_tag="VFE39_13725"
                     /pseudo
     CDS             139..681
                     /locus_tag="VFE39_13725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
     gene            755..1132
                     /locus_tag="VFE39_13730"
     CDS             755..1132
                     /locus_tag="VFE39_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HET6259703.1"
                     /translation="MPLMFLNGTAMSDGADHHLVGGAPLVTRTTTAPRYRFHAVNGRY
                     PALEDVGDGGAAVQGEVYDLSYEQLREVLLPGEPDGLELGVIELADGSGSLAMLLRRE
                     YAQLPPLTDISQQASWRTYQEQA"
     gene            1129..2448
                     /gene="allB"
                     /locus_tag="VFE39_13735"
     CDS             1129..2448
                     /gene="allB"
                     /locus_tag="VFE39_13735"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HET6259704.1"
                     /translation="MILIRAQRAVVDGVEQSAAVLVHGERIEAVLPFDASIDAREVVL
                     ARDEVLLPGLVDTHVHVNEPGRTEWEGFASATRAAAAGGVTTIVDMPLNALPPTVDVA
                     ALEIKRKAADGQCRIDVGFWGGAVPGNAGELRALHDAGVFGVKCFLLDSGVPEFPPLV
                     GEELAVALREVAAFGGLMIVHAEDGTLIRPATGRRYADFVASRPRAAEDSAIATLLRA
                     ARDTGARVHVVHLSSADALPQLAAARAEGVHVTVETCPHYLALTAEEVPDGDTRFKCC
                     PPVREAGNRDALWQGLADGTIDTVVSDHSPCTADMKRLEAGDFGEAWGGIASLQLGLP
                     VVWSEARRRGHGLADVVMWMAERPAALVGLSRKGRIAAGQDADLTVFAPEATFVVGRL
                     HHKNPITPYAGRELHGEIRSTWLRGEPVEVEGAAPPRGRLLNRQDAR"
     gene            2445..3449
                     /gene="alc"
                     /locus_tag="VFE39_13740"
     CDS             2445..3449
                     /gene="alc"
                     /locus_tag="VFE39_13740"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005154774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HET6259705.1"
                     /translation="MTDFRQLPDLALRTLGGGVVWANDEAFAARENLIIPGPALFDRG
                     TFGHKGKVYDGWETRRRREPGHDEAIIRLGAPGIVSGVVVDTSWFTGNYPPEAAVDGL
                     AVDGHPTAADLLADNGWEPLLPRTAIKGDTENVFAVTSDRRITHVRLRIHPDGGVARL
                     RVHGEALPDPRLLDALGTVDLAAIENGGRVIACSNLFYSSPNNLLVPGPPRSMGEGWE
                     TARRRDDGNDWVEVALTGESVITVAEVDTSYFLHNAPGWAALRGRRGDGEWFDLVPRT
                     ALQPDTRHRFALAADAAHPATHVRMDIFPDGGMARLRMHGRLTPEGRADLDRRWTGGR
                     "
     gene            3446..3610
                     /locus_tag="VFE39_13745"
     CDS             3446..3610
                     /locus_tag="VFE39_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259706.1"
                     /translation="MNVPFLRSPHETGTGKIPFDQHFEALARVGYTGWVGLEYMPSTS
                     SERAFDWLAR"
     gene            3719..4042
                     /locus_tag="VFE39_13750"
                     /pseudo
     CDS             3719..4042
                     /locus_tag="VFE39_13750"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
     gene            complement(4169..5431)
                     /locus_tag="VFE39_13755"
     CDS             complement(4169..5431)
                     /locus_tag="VFE39_13755"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6259707.1"
                     /translation="MLLPHPLPLLPSLRLSAPALSGRIEAAVTLSTVARLVASGTAMS
                     KADLVPAAGLARTTVSTAVDELLARGVLRLDGTRPTAGRGRPADRLALSPRGGHVLLA
                     DIGASGAHLAVVDLSQRVLGHTHVDVDVTDGPEVVLRTVESHLTGLLGQAPDDVPVRA
                     VVIGLPGPVDGQLGVPVRPPIMPGWHAYPVTARLQQTYRCPAVLENDVNLRALGEARA
                     LPADQVPLLFVKVGTGIGGGLITREGVLHHGADGAAGDIGHVRVRGAPDDACVCGNVG
                     CIEAVASAGAIARRLAVAHGSTGRGGAGDVSIADVRALVGRGDPVALGLVREAAGLIG
                     ELVATLVHFYNPARVTIGGSLTAASDELLAGVRSVVYQRALPLATRNLVLTNSVLGEY
                     GGLAGAAVLGIERVLSADSIGVLTAGRS"
     gene            5555..7096
                     /locus_tag="VFE39_13760"
     CDS             5555..7096
                     /locus_tag="VFE39_13760"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019816227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6259708.1"
                     /translation="MTARMAEEPLLTMRGIVKQFPGVRALDGVDLDVAAGEVHCLLGQ
                     NGAGKSTLIKVLAGAHRPDTGEIVWAGDRVSFGSPQAADAAGIATIYQELDLVPGLSV
                     AENVVLGHEPARLGFTRPRDAEKVTRALLRRLGHPEIPPGRELGRLSAAGQQVVSMAR
                     ALARDARLIVMDEPSAVLDPEEVRNLFRVIRELTVDGVAVVYISHRLEEIREIGDRVT
                     VLKDGRSVARNLPARTTPTADVIRLMTGRTIEYVFPERPATQPGDVVLEVDGLARTGE
                     FAGVSFTVRAGEVVGLAGLVGSGRSEIIETVYGARKATAGTVRVRGKVLRAGSVPAAV
                     RAGMGLAPEERKSQGLLMAEPVYRNVSLPALARFARFGFLGRDADLTDAGAVTARLDV
                     RPADATRPVRTLSGGNQQKVVLARWLLQGCSVLLLDEPTRGVDVGARSEIYALIRRLS
                     AEGAAIVLVSSEVPEVLGLADRVLVVREGQVVHEAPSAELDEGRVLDLVMEALEPSAK
                     EGAGQ"
     gene            7267..8208
                     /locus_tag="VFE39_13765"
     CDS             7267..8208
                     /locus_tag="VFE39_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005157779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6259709.1"
                     /translation="MLDSGAGRNLGLVAVLALLCVVGVVTADTFLTTSNLLTILTSAS
                     IIGVLTVGVTFVIIGGGIDLSVGKVMALASVWATTVATQSYGPAVMVFCAVAVGVGAG
                     LINGLLIAYGRIVPFIVTLAMLISAQGLAERISGRRSQIVTDPAIAGIANTRVLRIPL
                     LVYIFAAVVAVGWVVLNRTTFGRRTFAIGGNAEAARLAGVDVRRHTVLLYAVSGLCAG
                     IAAIMIASLTTTGSSTHGTLYELDAIAAVIIGGTLLTGGRGTLIGSILGVLVFTTITN
                     LFVLNNLATEVQNIAKGVIIVVAVLIQARAQRSSTAG"
     gene            8246..9319
                     /locus_tag="VFE39_13770"
     CDS             8246..9319
                     /locus_tag="VFE39_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6259710.1"
                     /translation="MPDPLAVPGVLRRRGFLAGALGVGAGAALTACTSNEAPSPTTNA
                     PPAAAAGGGNAAPGRPVTIGFSAPAADHGWIAAIANNAQAQAQAYSDVTFEAVNPTND
                     ITQQIAAVQTLINRGVDALVILPNDGSQLTSVGRAAMQAGIPVVNLDRIFDSPLAYRT
                     WIGGDNYGMGVSAGNYVGERLRTAGVTNPVIGEIAGIDNLPLTQARSQGFADALGTYG
                     FRIGPRQAADFTAQGGQRVTANLLQAAPRLDAVWNHDDDQGIGVLAAINQAGRNEFFM
                     VGGAGSANAMRDIQADKTVLKATVTYSPSMASSAVALARLVAQGRGMGDLAEQEVPAS
                     ITLASATITRENVGNYLPLGFES"
     gene            9355..10500
                     /locus_tag="VFE39_13775"
     CDS             9355..10500
                     /locus_tag="VFE39_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005450398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6259711.1"
                     /translation="MVGYAFMGAAHSQAWRTARRFFDLPLQPDMAVLCGRNAAATTAA
                     AQRMGWRTTETDWKALLRRDDVQLVDVCTPGDTHAEIAVAALEAGKHVLCEKPLANTV
                     DEARAMVAAAERAQARGVRSMVGFNYRRVPAVALARRLVEQGRIGRVRHVRAVYLQDW
                     IVDPEFPLVWRLQAERAGSGALGDIGAHIVDMTQFVTGDRLTGVSALTETFITERPLP
                     EASSGLSASGSAERGPVTVDDAALFLGRFAGGAIASFEATRFATGRKNAIRWEINGSA
                     GSLAFDFESMNELSFYDGTEDPETAGFRRILVTEPTHPYAGAWWPPGHLLGYEHSFTH
                     EVVDLLRDLAAGTDPTPSFADGLQVQLVLDAVVRSAQSGSGWQAIAS"
     gene            10529..11533
                     /locus_tag="VFE39_13780"
     CDS             10529..11533
                     /locus_tag="VFE39_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HET6259712.1"
                     /translation="MARPITLFTGQWADLPFEEVARLAGEWGYDGLEIACWGDHFDPW
                     AAVEDDGYVQGRLDVLEKHGLSVYAISNHLTGQAVCDDPIDERHRGILPARVWGDGEP
                     EGVRRRAAEAMQVTARAAAKLGVDTVIGFTGSKIWKTVAMFPPVPQSMVDDGYRDFAD
                     RWNPILDVFDEVGVRFAHEVHPSEIAYDYWTTVAALEAVGRRPAFGLNWDPSHFVWQD
                     LDPVGFLWDFKDRIYHVDCKDAKRQVGNGRNGRMGSHLAWADPRRGWDFVSTGHGDVP
                     WEACFRMLNTIGYAGPISVEWEDAGMDRLVGAPEALEFVRRLAFDPPSAAFDAAFSSG
                     "
     gene            11684..12691
                     /locus_tag="VFE39_13785"
     CDS             11684..12691
                     /locus_tag="VFE39_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6259713.1"
                     /translation="MGTLAEGRRIASCLRGGIAGPDADAAVRGLRSLLDAEAVGIGGL
                     QGELTWSGRPADRVAAERLAGSVLRKDARSAAGGVAALPIHARDELAGVLVVVGRVPA
                     AAAREAALWVGEALERAVLESSADVAQQAELRALRAEISPHFVYNSLTTIASFVRSDP
                     ERARDLILDFAEYTRHSLARHGDYTTVAGEFRAIEAYLALARAVLGDRLRVQVRIAPE
                     ILPVALPYLVLQPLVENAVRHGVELPGASGLVQVSGEAAGSECVIAVEDDGPGMDPEY
                     AAAVLAGRGRPGSLGLVNVDRRLRTIFGAGYGLVIETAVGAGTRVLVRLPRFQPGVVA
                     S"
     gene            12688..13464
                     /locus_tag="VFE39_13790"
     CDS             12688..13464
                     /locus_tag="VFE39_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HET6259714.1"
                     /translation="MTGLRVLAVDDVAPALEELHRMLVEAPDVATVAVAADPIMALRR
                     IQAGPLDAVFLDIAMPAMTGMELASLLSRMTDPPVVVFVTAYEEHAASAYGIGAVDYL
                     LKPVSAERLAAALARVRRFTAAAAAGTMPAPVDALAAVPVELAGRTCFVRRDDIRFVE
                     AQGDHVRLHTPTGAHLVRMPISRLEEHWRPAGFSRVHRSYLLAVRAVRELRSDLSGGL
                     LAHTDLGDVPVSRRHARDLREQLMDAARRGAFDPANRPLR"
     gene            13536..13883
                     /locus_tag="VFE39_13795"
     CDS             13536..13883
                     /locus_tag="VFE39_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259715.1"
                     /translation="MTSPQTRLALAGSRTGVPPAPPHLAPADAERARRIHRRQLRYAL
                     IVLGLLAALLLGLPVLLAAAPRLDEVRFAGIPVSWLAVAVLPYPVLVALAWCQLRWAE
                     RTERPPRRARGRG"
     gene            13880..>14747
                     /locus_tag="VFE39_13800"
     CDS             13880..>14747
                     /locus_tag="VFE39_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HET6259716.1"
                     /translation="MIFAIVPVLLITLLIGARGVAAMRTTSDFLVASRRISPTVNAAA
                     VSGEYLSAASFLGVAGLVVKDGVGAFWYPVGFAAGYIAMLALVAAPLRRTGALTAPDF
                     AEARLGSPVLRRLAAVVVLVIGFLYLVPQFSAAGQVLAVVGGNPYWVGVVIAGAAVSI
                     TLALGGMRAATYVQAFQFALKLVLFIVPAIWLVLLVGPSTRANALDPVEFTRFPAATP
                     VQFRLDTELTITEPTRVSIAGGPQQVLPPGRYEAQAGSTWVFDVGAAVPQVGAGRPPG
                     GREWERPLLDPGG"
BASE COUNT         1955 a         5175 c         5516 g         2101 t
ORIGIN      
        1 ggctccgtcg ggcggtgccg gaactcgacg gtctcctcca cgacgtcgcc gccgagcacc
       61 tcggccagcg cgacgcgcac ctcgccgctc gtgtcgacga ccttgtcgcc gtccagccgg
      121 aggtccgccg gcgaacgggt cgccgcgatc gccgacctgg tcggcgagga ggcgctcgag
      181 cggacggtgg tgtggcggca ccgcccgacc acgaccctgg accccgagac gggacagggg
      241 aacgcgcacg tgcagttcgc tttcgcggcg caccgggccg tcgtcgacgt ggacgtcgag
      301 ctggggctgg tgaaggtggt cgcgctggac accgcgcagg acgtcggcag ggccatcaac
      361 ccggacgccg tgatcggcca gatccagggc ggcagcgcgc agggcatggg gttggcgctg
      421 atggaggagg ttgtcgtgac ggacggcaag gtgctgaacc cgtcgttcac cgactacctc
      481 atcccgacga tcctcgacat gccgccgatg cggatcaagg tgctggagta cgccgacccg
      541 cacgcccctt acggtgtccg tggtgtcggt gagcctccga cgatctccag cggtcccgcc
      601 gtcgcggccg ctgtccgggc agccaccggt aaggcgctgc ttcgcgttcc cgtccggccc
      661 gagcacatca ccggcgcctg acggcgcccg tgcgcggata cgggtgccgg agcactcgta
      721 tccgcgcacg agcacgacga aggagtgcaa agggatgcca ctgatgttcc tgaacggcac
      781 cgcaatgtct gacggcgcag accaccacct ggtcggcggg gcgccgctgg tcacccgcac
      841 caccaccgca ccgcgctacc gcttccacgc cgtgaacggg cgctaccccg ccctggagga
      901 cgtcggggac gggggagcgg cggtgcaggg cgaggtctac gacctgtcct acgagcagct
      961 gcgggaggtg ctgctgcccg gggagcccga cggcctggag ctcggcgtga tcgagctggc
     1021 cgacggcagc gggtcgctgg cgatgctgct gcgccgggag tacgcgcagc tcccgccgct
     1081 gaccgacatc tcgcagcagg cgagctggcg cacctaccag gagcaggcgt gatcctcatc
     1141 cgggcgcagc gcgccgtggt cgacggagtc gagcagtcgg ccgcggtgct ggtgcacggc
     1201 gagcgcatcg aggccgtgct gccgttcgac gcctcgatcg acgcgcggga ggtggtgctc
     1261 gcccgcgacg aggtgctgct ccccggtctc gtcgacaccc acgtgcacgt gaacgagccc
     1321 ggtcgcaccg agtgggaggg ctttgcctcg gccacccgcg ccgccgcggc cggtggggtc
     1381 acgacgatcg tggacatgcc gctgaacgcg ctgccaccga ccgtcgacgt ggccgcgctg
     1441 gagatcaagc gcaaggcggc ggacgggcag tgccgcatcg acgtcggctt ctggggcggc
     1501 gcggtgccgg gcaacgcggg cgagctgcgc gcgctgcacg atgccggggt gttcggcgtc
     1561 aagtgcttcc tgctcgacag cggcgtcccg gagttcccgc cgctggtcgg ggaggaactg
     1621 gccgtggcgc tgcgggaggt cgcggcgttc ggcggcctga tgatcgtcca tgccgaggac
     1681 ggcacgctga tccgtcctgc gactggccgt cggtacgccg acttcgtggc ctcacgcccg
     1741 cgggccgcgg aggacagcgc gatcgccacg ctgctgcggg cggcgcgcga taccggtgcc
     1801 cgggtgcatg tggtgcacct gtcctcggcc gacgcgttgc cgcagctcgc cgccgcgcgc
     1861 gccgagggcg tccacgtcac cgtggagacg tgcccgcact acctcgccct gaccgccgag
     1921 gaggtgcccg acggggacac ccgcttcaag tgctgcccac cggttcgtga ggccggcaac
     1981 cgggacgcgc tgtggcaggg cctcgccgac gggacgatcg acacggtcgt ctccgaccac
     2041 tcgccctgca cggccgacat gaagcgcctg gaggcgggtg acttcggcga ggcgtggggc
     2101 gggatcgcgt cgctgcagct cgggttgccc gtggtctggt ccgaggcacg tcgccgcggc
     2161 cacggcctcg cggacgtcgt catgtggatg gccgagcgcc cggcggcgct ggtcgggtta
     2221 tcccggaagg gccgcatcgc tgctggtcag gatgccgatc tgaccgtgtt cgcccccgaa
     2281 gcgaccttcg tggtcgggcg gctgcaccac aagaacccga tcaccccgta cgccgggcgg
     2341 gagctgcacg gcgagatccg gagcacctgg ctccggggcg aaccggtcga ggtggagggg
     2401 gcagctccgc cccgtggccg cctcctgaac aggcaggacg cacgatgact gacttccggc
     2461 agctgcccga cctcgcactg cgcacgctcg gcggcggcgt cgtgtgggcc aacgacgagg
     2521 cgtttgccgc gcgcgagaac ctgatcatcc cggggcctgc gctgttcgac cggggcacct
     2581 tcgggcacaa gggcaaggtg tacgacggct gggagacccg gcgccgccgc gagcccggac
     2641 acgacgaggc gatcatccgc ctgggcgccc cggggatcgt ctcgggcgtc gtggtcgaca
     2701 cgtcctggtt caccggcaac tacccaccgg aggcagcggt cgacgggctc gcggtcgacg
     2761 gtcacccgac ggccgccgac ctcctcgcgg acaacggctg ggagccgctg ctgccgcgca
     2821 ccgcgatcaa gggcgacacc gagaacgtgt tcgcggtgac gagcgaccgg cggatcacgc
     2881 acgtgcgtct gcggatccat ccggacggtg gcgtggcccg gctccgggtg cacggcgagg
     2941 cgctgccgga cccgcggctg ctcgacgcgc tcggcacggt cgaccttgcg gcgatcgaga
     3001 acggtggcag ggtcatcgcc tgctccaacc tcttctacag ctcgccgaac aacctcctgg
     3061 tccccggccc accccgctcg atgggggagg ggtgggaaac ggcccggcgt cgcgacgacg
     3121 gcaacgactg ggtggaggtg gcccttaccg gcgagtccgt gatcacggtg gcggaggtcg
     3181 acacaagcta cttcttgcac aacgcgccgg gctgggccgc gctgcgcgga cgccggggcg
     3241 acggcgagtg gttcgacctc gtgcctcgca cggcgctgca gcccgacacc cgtcaccggt
     3301 tcgcgctcgc cgcggacgcc gcccacccgg ccacccatgt ccgcatggac atcttccccg
     3361 acgggggcat ggcccggttg aggatgcacg ggcggctcac tccggagggc agggcggacc
     3421 tcgaccggcg gtggacgggg ggccgatgaa cgtgcccttc ctgcgctcgc cccacgagac
     3481 tggcaccggc aagatcccgt tcgaccagca ctttgaggcc ctcgcccgcg tcggctacac
     3541 cggctgggtc ggcctcgagt acatgccgtc cacgtcgagc gagcgggcct tcgactggct
     3601 cgctcgatga gcgccgcgga ggggtcgggc cgcgcggaca gggccacggt cgccgtgatc
     3661 ggcctcggca tcgtgggggg cccgatggcg gccaacctgg ttggccgccg gtcaggacgt
     3721 gatcggcttc aaccggtcgc gtcccgcggt cgacaggttg gtcgagctcg gcaggcgggc
     3781 ggcggagagt accgccgagg ccgtccgcga ggccgacgtg gtgatcacga ttctcccgga
     3841 cagccccgac gtcgaggcgc tggcgctggg tgaggacggg atctacgcca acgccgagcc
     3901 gggcacgctg cacatcgact gctccaccat ccgccccgac gtgggccgcc gcctcgccgc
     3961 ggccggggcc gagcgccgga tccgagtggt ggactccccg gtcagcggcg gcgaggcggg
     4021 cgcggtcgag ggcacgttgt cgatcatggt gggcggcgag ccggccgacg tcgaggcggc
     4081 acggcccttt ctggacgtgg tcaaggccgc cggcatgcgg ttgcgcgagt tcagcccggg
     4141 ggcgggccgg cacgggagac tgacccgatc aggagcgtcc ggcggtcagc acgccgatcg
     4201 agtcggccga cagcacgcgt tcgatgccca gcacagcagc gcccgcgagg cccccgtact
     4261 cgcccagcac actgttggtc agcacgaggt tgcgggtggc cagcggtagc gcccgttggt
     4321 agacgacgct gcgcacgccg gcgagcagct catcgctcgc tgccgtgagg gacccgccga
     4381 tcgtcacccg cgccgggttg tagaagtgca cgagcgtggc cacgagctca ccgatcagcc
     4441 cggcggcctc gcggaccaga ccgagcgcga ccgggtcgcc gcggcccacc agcgcgcgga
     4501 cgtcggcgat cgacacgtcc cccgccccgc cccggcccgt gctgccgtgc gccacggcca
     4561 gcctgcgcgc gatggccccc gccgacgcca cggcctcgat gcagccgacg ttgccgcaga
     4621 cgcacgcgtc gtcgggcgcc ccgcgcaccc gcacatgccc gatatcgccc gcggcgccgt
     4681 cggcgccgtg gtgcagcacc ccttcccgcg tgatcaaccc gccgccgatg cccgtgccga
     4741 ccttgacgaa cagcagcgga acctggtcgg cgggcagtgc gcgggcctct ccgagcgcgc
     4801 gcaggttgac gtcgttctcc aggaccgcgg gacaccgata cgtctgctgc agccgggctg
     4861 tgacgggata ggcgtgccac ccgggcatga tcggtggccg taccgggacg ccgagctggc
     4921 cgtccaccgg gcccggcagc ccgatgacga ccgcgcgcac cgggacgtca tccggtgcct
     4981 ggccgagcag ccccgtgagg tgtgactcga cggtgcgcag cacgacctcc ggtccatcgg
     5041 tcacgtcgac gtcgacgtgc gtgtggccca gcacgcgctg gctgaggtcg acgacggcca
     5101 ggtgcgcgcc gctcgcgccg atgtcggcca acaggacgtg cccgccccgc ggggacagcg
     5161 ccagccggtc ggcgggacgg ccgcgccccg ccgtgggccg cgtgccatcc agccggagca
     5221 ccccgcgagc gagcagctcg tccacggcgg tgctcacggt ggtgcgggcg aggcccgccg
     5281 cggggaccag gtcggccttg ctcatggcgg tgcccgaggc gaccagccgg gccactgtgc
     5341 tgagggtgac ggcggcctcg atgcggccgg agagcgcggg cgcgctcagg cgcagcgatg
     5401 gcagcagtgg tagcgggtgc ggcagaagca tcacggtccg atttcgtcgg aaaaacagcg
     5461 caaagaagca ttattcggtc gttgtaaggc actggtcaat gctcctacgg ttccaccgcc
     5521 ggtaaccacg tcgaggtggg gtcgaggtgg gggaatgaca gccaggatgg cggaggaacc
     5581 gctgctgacg atgcgcggca tcgtcaagca gttcccgggc gtacgcgccc tggacggagt
     5641 ggacctcgac gtggcggcgg gggaggtgca ctgcctcctc ggtcagaacg gggccggcaa
     5701 gtcgaccctg atcaaggtgc tggccggggc gcaccggccg gacaccggcg agatcgtgtg
     5761 ggcaggtgac cgcgtctcgt tcggctcccc ccaggccgcc gacgcagcgg ggatcgccac
     5821 catctaccag gagctggact tggtgcccgg gctcagtgtg gccgagaacg tggtgctcgg
     5881 ccacgagcct gcccgtctcg gtttcacccg gccgcgcgac gccgagaagg tgaccagggc
     5941 gttgctgcgc cgcctcggcc accccgagat cccgcccggt cgcgaactgg ggcgcctctc
     6001 ggcggcaggc cagcaggtgg tgagcatggc ccgcgcgctc gcgagggatg cgcgcctgat
     6061 cgtcatggac gagccgtcgg cggtgctcga cccggaggag gtgcgcaacc tgttccgggt
     6121 gatccgggag ctcacggtcg acggcgtggc cgtggtctac atctcccatc ggctcgagga
     6181 gatccgcgag atcggcgacc gggtcacggt cctcaaggac gggcgcagcg ttgcccggaa
     6241 cctgcccgcc cgcaccacac cgactgccga cgtcatccgg ctgatgacgg gccggaccat
     6301 cgaatacgtc tttccggagc gcccggccac gcagcccggg gacgtggtgt tggaggtgga
     6361 cgggctcgcg cgcacgggcg agttcgccgg ggtctccttc accgtccgcg ccggtgaggt
     6421 cgtcgggctg gccgggctgg tcgggtcggg tcgctcggag atcatcgaga ctgtctacgg
     6481 ggcgcgcaag gccaccgccg gcacggtgcg cgtgcggggg aaggtgctac gcgcgggctc
     6541 ggtgcccgcc gcggtccggg ccgggatggg cctggcgccc gaggaacgta aaagccaggg
     6601 cctgctgatg gccgagcccg tgtaccgcaa cgtctcgctg cccgcactcg cccggttcgc
     6661 ccggttcggc ttcctgggca gggacgccga cctcaccgac gccggtgcgg tcacggcgcg
     6721 gctcgacgtc cgcccggccg acgccacccg cccggtccgg acgctctccg gcggcaacca
     6781 gcagaaggtc gtgctggccc gctggctgct gcagggatgc tcggtcctgc tcctggacga
     6841 gcccacccgc ggcgtggacg tcggggcgcg cagcgagatc tacgcgctga tccggcggct
     6901 ctcggccgag ggcgcggcga tcgtgctggt gtccagcgag gtgcccgagg tgctcggcct
     6961 cgccgaccgg gtgcttgtcg tccgcgaggg ccaggtcgtc cacgaggccc cgagcgccga
     7021 gctcgacgag ggtcgggtcc tcgacctggt catggaagcg ctcgaaccat ctgcgaagga
     7081 aggtgccggc cagtgagcga gcagggcaca gcatcgggca cggtaccggg cggagcgcag
     7141 gcggagcccg catcggcgcc gggcggagcg caggcggagc cggtatcggc accggccgcc
     7201 gacgagcgga gccgggtcga gcaggcggcg cgcgtcgcgg agcctgcccg gtcgcccctc
     7261 agtcggctgc tcgacagcgg tgcgggtcgc aacctcgggc tcgtcgcggt actcgcgctc
     7321 ctgtgcgtgg tcggcgtcgt cacggccgac acgttcctga ccacctcgaa cctgctgacg
     7381 atcctgacgt cggcttcgat catcggggtg ctgaccgtcg gggtcacgtt cgtgatcatc
     7441 ggcgggggta tcgacctgtc ggtcggcaag gtgatggcgc tggcgtcggt gtgggccacg
     7501 actgtggcga cccagtcgta cggcccggcc gtgatggtgt tctgcgcggt cgccgtcggc
     7561 gtcggggccg gcctgatcaa cggcctgctg atcgcctacg gccggatcgt ccccttcatc
     7621 gtcacgctgg cgatgctgat ctccgcccag ggtctggccg agcggatctc cggtcggcgc
     7681 agccagatcg tcaccgaccc cgcgatcgcg ggcatcgcga acacgcgcgt gctccggatc
     7741 ccgctgctgg tctacatctt cgcggcggtg gtggcggtcg gttgggtggt gctcaaccgc
     7801 accacgttcg gccgacggac gttcgccatc ggcggcaacg cagaggcggc ccggctggcc
     7861 ggggtcgacg tccgcaggca caccgttctg ctgtacgccg tgtccgggct gtgtgccggc
     7921 atcgccgcga tcatgatcgc gtcgctgacc accaccgggt cgagcaccca cggcacgctg
     7981 tacgagctcg acgcgatcgc cgcggtgatc atcggcggca cgctgctcac cggcggccgt
     8041 ggcacgttga tcggctcgat cctcggcgtg ctcgtcttca cgacgatcac caacctgttc
     8101 gtgctcaaca acctggcgac cgaggtccag aacatcgcca agggcgtgat catcgtcgtc
     8161 gccgtgctga tccaggcccg tgctcagcgc tcgtcaactg ccggttgaaa ccgcaggtcg
     8221 accgacccca acgaaggagt ccaccatgcc cgaccccctg gccgtacccg gtgtcctgcg
     8281 gcgccgtggt ttcctcgccg gcgccctcgg cgtcggggcc ggtgccgcgc tgaccgcctg
     8341 caccagcaac gaggccccat cgccgaccac gaacgcgccc ccagcagccg ccgccggcgg
     8401 cggcaacgcc gctccaggca ggccggtgac catcggcttc tccgcgcccg ccgccgatca
     8461 cgggtggatc gccgccatcg cgaacaacgc gcaggcgcag gcgcaggcct actccgacgt
     8521 gacgttcgag gccgtcaacc cgaccaacga catcacccag cagatcgccg ccgtccagac
     8581 cctgatcaac aggggcgtgg acgcgctggt gatcctgccc aacgacggca gccagctcac
     8641 ctcggtcggg cgtgcggcga tgcaggccgg cattccggtg gtcaacctcg accggatctt
     8701 cgactcgccg ctggcctacc gcacgtggat cggtggggac aactacggca tgggtgtaag
     8761 cgcgggcaac tacgtcggcg agcggctgcg caccgcgggc gtgaccaatc cggtgatcgg
     8821 cgagatcgcg ggcatcgaca acctcccgct gacccaggcc cgcagccagg gcttcgccga
     8881 cgccctgggc acctatggct tccggatcgg cccccggcag gcggccgact tcacggcgca
     8941 gggcggccag cgggtcaccg cgaacctgct gcaggcggcg ccgcggctcg acgccgtctg
     9001 gaaccacgac gacgaccagg gcatcggcgt cctcgccgcg atcaaccagg ccgggcgcaa
     9061 cgagttcttc atggtgggcg gagcggggtc ggccaacgcg atgcgcgaca tccaggccga
     9121 caagacggtt ctcaaggcga cggtcaccta cagcccgtcg atggcgtcgt ccgcggttgc
     9181 gctggcgcgg ctcgtggcgc agggtcgggg catgggcgat cttgcagagc aggaggtgcc
     9241 cgcctccatc acgctggcct cggcgacgat cacccgcgag aacgtcggca actacctccc
     9301 gctcgggttc gagtcctgag cgccgtgggt agcgaacggg tgctcggggt cggcatggtg
     9361 ggctacgcct tcatgggcgc cgcccactcc caggcatggc ggacggcgcg acggttcttc
     9421 gacctgccac tgcaaccgga catggcggtg ctgtgcggcc gcaacgccgc ggcgaccacg
     9481 gcggcggcgc agcggatggg ctggcgcacc acggagacgg actggaaggc gctgctccgg
     9541 cgcgacgacg tccagctcgt cgacgtctgc acgccggggg acacgcacgc ggagatcgcg
     9601 gtcgccgcgc tggaggccgg caagcacgtg ctgtgcgaga agccgctggc caacaccgtc
     9661 gacgaggcgc gggcgatggt ggccgccgcg gagcgggcgc aggccagggg cgtgcgcagc
     9721 atggtcggct tcaactaccg gcgggtgccc gccgtcgcgt tggcccggcg gctggtggag
     9781 caggggcgga tcggccgggt gcgccacgtg cgggcggtgt atctgcagga ctggatcgtg
     9841 gacccggagt tcccgctggt ctggcggctg caggcggagc gggccgggtc cggggcgctc
     9901 ggcgacatcg gtgcgcacat cgtcgacatg acccagttcg tcaccggcga ccggctcacc
     9961 ggcgtctcgg cgctgaccga gacgttcatc accgagcggc cactgccgga ggcgtcgagc
    10021 gggttgtcgg cgtccggttc cgcggagcgc ggcccggtga ccgtcgacga cgctgcgctc
    10081 ttcctcggcc gcttcgcggg cggggccatc gcgagcttcg aggccacccg gttcgccacg
    10141 ggtcgcaaga acgcgatccg gtgggagatc aacggcagcg cgggctcgct ggcgttcgac
    10201 ttcgagtcga tgaacgagct gtccttctac gacggcacgg aggacccgga gacggccggg
    10261 ttccggcgga tcctggtcac cgagcccacc cacccctacg cgggcgcgtg gtggccgccc
    10321 ggccacctgc tcggttacga gcactcgttc acgcacgagg tggtcgacct gctgcgcgac
    10381 ctcgcggcgg gcaccgatcc gacgccgtcg ttcgccgacg gcctgcaggt gcagctcgtg
    10441 ctcgacgccg ttgtccggtc cgcgcagagt ggcagcggct ggcaagcgat cgcatcctga
    10501 tcatcctcgc cgaaaggggc accgcgccat ggcgcgaccg atcacgttgt tcaccggcca
    10561 gtgggccgac ctgccgttcg aggaggtcgc ccggctggcg ggggagtggg gctacgacgg
    10621 cctggagatc gcctgctggg gcgaccactt cgatccctgg gccgccgtgg aggacgacgg
    10681 gtacgtgcag ggccgcctcg acgtgctgga gaagcacggg ttgtccgtgt acgccatctc
    10741 caaccatctc accgggcagg ccgtctgtga cgacccgatc gacgagcgac accgggggat
    10801 cctccccgcg cgggtgtggg gcgacggcga gccggagggc gtgcggcggc gagcggccga
    10861 ggcgatgcag gtgaccgccc gggctgccgc gaagctcggc gtcgacacgg tcatcgggtt
    10921 caccgggtcg aagatctgga agaccgtggc gatgttcccg ccggtgccgc agtcgatggt
    10981 ggacgacggc tatcgcgact tcgccgaccg gtggaacccg atcctcgacg tgttcgacga
    11041 ggtgggcgtc cggttcgccc acgaggtgca cccctcggag atcgcctacg actactggac
    11101 gaccgtggcg gcactcgagg ccgtcgggcg ccggccggcg ttcgggctga actgggatcc
    11161 gagccacttc gtctggcagg acctcgaccc cgtcgggttc ctgtgggact tcaaggaccg
    11221 gatctaccac gtcgactgca aggacgcgaa gcgccaggtg ggcaacggcc gcaacgggcg
    11281 gatgggctcg cacctggcat gggccgaccc gcggcgcgga tgggacttcg tctccacagg
    11341 gcacggcgac gtcccgtggg aggcgtgctt ccggatgctc aacaccatcg gctacgccgg
    11401 cccgatctcg gtggagtggg aggacgcggg gatggaccgg ctcgtcgggg cgccggaggc
    11461 gctggagttc gtgcgccggc tcgcgttcga tccgccgtcg gctgccttcg acgcggcgtt
    11521 cagcagcggc tgacctcgtc acgggctggc cctgatgccc ggcgtgacct accgtcgcgc
    11581 tgttcggtga cggaggggct gcatggtgga ggtcggtgcg gtgcgggtgc ccggcagcgg
    11641 ccggccccgc cggtggcgcg gttccggctg ggggcgcgac gcgatgggca ctctggcgga
    11701 gggcaggcgc atcgccagtt gtctgcgcgg cggtatcgcc ggacccgacg cggacgccgc
    11761 cgtgcgaggc ctgcgcagcc tgctcgacgc cgaggcggtg ggcatcggcg ggctgcaggg
    11821 cgagctgacg tggtcggggc gacccgccga ccgggtcgcc gctgagcgac tcgccggcag
    11881 cgtgctgcgc aaggatgcgc gctcggcggc tggcggtgtc gccgcgttgc ccatccacgc
    11941 gcgggacgag ctggccgggg tgttggtcgt ggtcgggcgg gtgcctgcgg ccgcggcgcg
    12001 cgaggcggcc ctgtgggtgg gggaggcact ggagcgggcg gtcctggaga gctccgccga
    12061 cgtcgcccag caggcggagc tgcgggcgct gcgggcggag atctcgccgc atttcgtcta
    12121 caacagcctc acgacgatcg cgtcgttcgt ccgttccgac cccgagcggg cccgtgacct
    12181 cattctggac ttcgccgagt acacgcggca cagcctcgcc cgccacggcg actacaccac
    12241 ggtcgccggt gagttccgcg cgatcgaggc ctacctcgcg ctcgcccgcg ccgtgctcgg
    12301 cgaccgcctg cgggtgcagg tgcggatcgc gccggagatc ctccctgtcg cgctcccgta
    12361 cctggtgctc cagccgctgg tggagaacgc ggtgcggcac ggggtggagc tgccgggtgc
    12421 cagcgggctc gttcaggtca gtggcgaggc cgccggcagc gaatgcgtga tcgccgtgga
    12481 ggacgacggg cccggcatgg atcccgagta cgctgccgcc gtcctggcgg ggcgcggcag
    12541 gcccgggagc ctggggctgg tcaacgtcga ccgcaggttg cggacgatct tcggggccgg
    12601 ctacgggctg gtgatcgaga cggcggtggg cgcgggcacc cgggtactgg tgcgcctgcc
    12661 gcgtttccag ccgggggtgg tggcttcatg accgggctgc gggtgctcgc ggtggacgac
    12721 gtcgcccccg cgctcgagga gttgcacagg atgctggtgg aggcgccgga cgtggccacc
    12781 gtggcggtgg cggcagaccc catcatggcc ctgcggcgga tccaggccgg cccgctcgac
    12841 gcggtgttcc tcgacatcgc gatgcccgcg atgacgggaa tggagctggc atcgctgctc
    12901 tcccgcatga ccgacccacc ggtggtcgtg ttcgtcaccg cctatgagga gcacgcggcg
    12961 tcggcctacg gcatcggcgc tgtcgactac ctgctcaaac cggtcagcgc ggagcgactc
    13021 gccgccgcac tcgccagggt gcggcgcttc actgcggctg cggccgccgg cacgatgccc
    13081 gctccggtcg acgcgctcgc cgcggttccg gtggagctcg ccggccgcac ctgcttcgtc
    13141 cggcgcgatg acatccgctt cgtcgaggcg cagggcgacc acgtccggct gcacaccccg
    13201 acgggggcgc acctcgtgcg gatgccgatc tcccgcctcg aggagcactg gcggcccgcc
    13261 ggcttcagcc gtgtgcaccg cagctatctg ctcgctgtgc gtgccgtccg cgagctccgc
    13321 agcgatctct cgggcgggct gctcgcccac accgacctcg gcgacgttcc ggtcagccgc
    13381 cgccacgccc gtgatctgcg tgagcagctg atggacgcgg cgagacgcgg agcgttcgat
    13441 cccgcgaacc ggccgctccg atgacgagac aggttgcgtg aggggaccgg ccggatgacg
    13501 gggcaccttc gatgacacga cagcgccgga ttgcggtgac gagcccgcag acgcgcctcg
    13561 cactcgcggg tagccgtacg ggcgtcccac ccgcaccgcc gcatctcgct cccgccgacg
    13621 ccgagcgggc ccggcggatc caccgccggc agctgcggta cgcgctcatc gtgctcgggt
    13681 tgctggccgc gctgctgctc ggccttcccg tcctgctcgc cgccgccccg cggctggacg
    13741 aggtgcggtt cgccggaatc ccggtgtcct ggctggcggt ggccgtgctg ccgtacccgg
    13801 tgctcgtcgc gttggcctgg tgccagctgc gctgggccga gcgaacggaa cggccgccgc
    13861 gccgggcgag aggtcgcggg tgatcttcgc catcgtcccg gtcctgctga tcacgctgct
    13921 gatcggggcg cgcggagtgg cggcgatgcg caccacctcg gacttcctgg tggcctcgcg
    13981 ccggatctcc ccgacggtga acgcggctgc cgtctcgggg gagtacctgt cggcggcgtc
    14041 gttcctcggt gtcgcgggcc tcgtcgtcaa ggacggggtc ggcgcgttct ggtaccccgt
    14101 cggcttcgcg gcgggataca tcgcgatgct cgcgctcgtg gccgcgccgc tgcgccgtac
    14161 cggtgcgctc accgcgccgg acttcgccga ggcgcggctg ggttccccgg tgctgcgcag
    14221 gcttgctgcg gtggtggtgc tggtgatcgg gttcctctac ctggtgccgc agttcagcgc
    14281 cgccgggcag gtgctcgccg tcgtcggcgg caacccctac tgggtggggg tcgtgatcgc
    14341 cggcgcggcc gtcagcatca cgctcgccct gggcgggatg cgcgcggcga cctacgtcca
    14401 ggcgttccag tttgcgctca agctcgtcct gttcatcgtc ccggcgatct ggctggtgct
    14461 gctcgtgggc ccgagcaccc gcgccaacgc tctcgatccg gtcgagttca cgcggttccc
    14521 cgcagccacc ccggtgcagt tccggctgga caccgagctc acgatcaccg agccgacccg
    14581 cgtgtccatt gcgggcgggc cacagcaggt gctccctccg ggacgctacg aagcgcaggc
    14641 cgggagcacc tgggtgttcg acgtcggggc ggccgtgccg caggtcggag ccgggcggcc
    14701 gcctggcggt agggagtggg aacggccgct gctcgatccc ggcgggt
//
LOCUS       DASRSC010000280        23666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3876833,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000280 DASRSC010000000
VERSION     DASRSC010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 23666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23666
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3876833"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VFE39_13805"
     CDS             <1..907
                     /locus_tag="VFE39_13805"
                     /inference="COORDINATES: protein motif:HMM:NF018657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HET6259717.1"
                     /translation="AGEIAVLAKVHHALGDGLAVTDALIRLLTDEAPPPVGPPAQDRD
                     PPTGAADRLRRAATVVRGITSLAIAGGAGTSPLAGPVTSAAHRRAGVCFDGAQVRAAA
                     RAHGVGTTVLLLAVVAETLHSLLAERDPDAVPATVRTMVPITTRTSATVRSRALGNRT
                     AAVSIDLPVGPMSPAQRVALVGTALAAGSSAGQPDGAAAVLRILGLLPKQVQALLVRP
                     IYGHRFFHALASVMPGSRRPLHSRGGLIREVYPVLPLADRVGLAVGALHWGRTTAIGI
                     TADPGLVPEIGGIPDRVKASLSLMT"
     gene            999..1946
                     /locus_tag="VFE39_13810"
     CDS             999..1946
                     /locus_tag="VFE39_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6259718.1"
                     /translation="MDTGTALVIAVPAAVLGAAGFGLASAAQQRATKEVAVTPTLSPR
                     LIVELLARPMWLLGLVATIVALALQIVALAFGPLAVVQPVLVTGVAFAAVFSALMQGR
                     HPDPLILLGALCCAAGLSAFLLLARPTAGTSDYGQFDLLASLPLAVVLTAVVIGCLVY
                     AAAVQHSSRVLALALATGVLYGVTAGLMKIITSQLRVGLDEPFRHPVLYVVCIIGPIG
                     FLLSQNTFQQGQLVAPAVAVITSVDPVVGVLIGVGWLGEQIDSSPALLAGECLAALVV
                     IGSIALITARGSWLLQHRLELTPQSALMPRRRAGRPISG"
     gene            1943..2689
                     /locus_tag="VFE39_13815"
     CDS             1943..2689
                     /locus_tag="VFE39_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6259719.1"
                     /translation="MRPLPRNAVVTGASSGIGAAVAQRLAHDGCTLLLVGRDRARLDA
                     VAARTGGRSVLADLREPAGIEAVCDAADGWVELLVHGAGVGAAGPLADTPSDVIDELL
                     AVNLVAPVQLTRALLPTLRAACGHVVFVASVAAIGVAHEAAYSATKAGLRGFADALRV
                     ESDIGVTTVLPGAVETPYYERRGQPYHRRFPRPIGPERVAAALVDAVRRNRAEVFVPR
                     WLTVGARVHGAAPALFAHLSRRMDRREQLS"
     gene            complement(2601..3938)
                     /locus_tag="VFE39_13820"
     CDS             complement(2601..3938)
                     /locus_tag="VFE39_13820"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET6259720.1"
                     /translation="MFTVVALEFRAVVRRGHTLLAVLGAGATWAAAEYLVAVLTPTGS
                     NWTIANSQADNLPVLQVVSFTGPWGVAFVLFTTTAALAALTAPSSTGTARRRAGAVGA
                     LVLAEATPGQVVVFPEKDIHVEDATLPGVVARFAAVARERDVVLVLGVEHRSGAHLYN
                     TALTFPRDGSAPLVYHKRYPVPGVEDALTPGGAPGFLPGDGRGGVAICADMGQVSLGR
                     EYGRAGVGLKAVPALDFHVDAWAQSRVQLLRGVENGFSVARAARHGYLTLSDSNGRVL
                     AQAASTAGIRAASVTADLPSAGGGTPYSRAGDWFAWLCLALSAAALTAALTPPRRTPH
                     RSLGRHPSAGPVSQPPARSPGPPDPDPGSMICTSSTWPPSGWRWRAPVRMSGALLEGS
                     SMSCVLLGPSARTSATPSGLSADPISSAARGGPCGVTGARRARVPPRAPARPL"
     gene            complement(4155..4727)
                     /locus_tag="VFE39_13825"
     CDS             complement(4155..4727)
                     /locus_tag="VFE39_13825"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6259721.1"
                     /translation="MSTRERIAEAARRIVVEEGSAAVSMRRVGKSVGLSQMATYRHYP
                     SRDALLAAVADACFAELAEGWGGHPWSGDDDGALERLLTEHLDFALGQPHLYVFLFTE
                     NRTGARAFPHDFRSGASPTLTPVADALTAGVRRGVFVPHDVWEVALSIAAQLHGLVEM
                     YHGGRIGLAESEFRALCSRAVERILDGVRR"
     gene            complement(4829..5287)
                     /locus_tag="VFE39_13830"
     CDS             complement(4829..5287)
                     /locus_tag="VFE39_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259722.1"
                     /translation="MTVGLVLAAVGLAAACSSHPATSAAPAAISSTVAGAPTVATTEP
                     SSTPSAQQPAGGNRSDAGTDLEAVNCGKVGPTGGAEVDLIADGTAAGTVGCTEAINII
                     SDYYRDAPTMSEGTAHRLVVQGWTCLADTGAFGTGTIGCSKDGFAFHTQP"
     gene            complement(5448..6686)
                     /gene="hutI"
                     /locus_tag="VFE39_13835"
     CDS             complement(5448..6686)
                     /gene="hutI"
                     /locus_tag="VFE39_13835"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HET6259723.1"
                     /translation="MIESWRSSHGATLITGVAELVTNDPSLGAGPLGLVPDAALVVQD
                     GAVAWVGPAAAAPAADQRVDVGGRAVLPGFVDSHAHLVFAGERSAEFEARMSGVPYDG
                     GGIASTVRATREADEEQLRSRLRTLVGEMRAQGTTTVEVKSGYGLTVADEVRALRLAR
                     EVTDETTFLGAHVVPAEYGGRVDDYVALVTGPMLAACAPHARWIDVFCEPASPHAFDG
                     AASRVVLEAGRDAGLGLRVHGNQLGAGPGVQLAVELGAASVDHCTYLTDADVDALASA
                     DTVATLLPGVEFSTRHPYPDGRRLLDAGVTVALATDCNPGTCFSSSMSLCVALAVREM
                     RMTPVEAVLAATAGGAAALRRTDVGRLGVGARADLAVLDAPSYRYLAYRPGVPIVRTL
                     ETGSVSRAPGSPLEPDARFR"
     gene            complement(6706..8049)
                     /locus_tag="VFE39_13840"
     CDS             complement(6706..8049)
                     /locus_tag="VFE39_13840"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HET6259724.1"
                     /translation="MTVEPEGQAFWCEHAWLGQGPVPRVRVAVRDARIAAVETGVDPA
                     PGDERLPGLVLPGFANAHSHAFHRALRGRTHDRGGTFWTWRDRMYAVAARLDPDSYLA
                     LARAAYAEMSLAGVTVVGEFHYLHHGPGGTPYADPNEMGGALVQAAADAGVRLTLLDT
                     CYLAGGLTAAGHTPLDEVQTRFSDGDAAHWAARVDRLEDSDTLRIGAAIHSVRAVPAA
                     QLPQVASATAGRALHVHVSEQPQENEVCEAFYGRTPAALLADHGVLGPSTTAVHATHL
                     TLPDIALLGGSGTTVCACPSTEADLADGVGPFRALRDAGSPLSVGSDQHVMSDLLAEA
                     RLVEAHERLTSGKRGRFRPAALVDALTAQGHACLGWPDAGRIAPGQRADLVAVRLDTP
                     RTAGAAPEQVVLVATAADIDTVLVDGVPVVAGGVHRLGDVGALLRGAIEPFWSMR"
     gene            complement(8046..9290)
                     /locus_tag="VFE39_13845"
     CDS             complement(8046..9290)
                     /locus_tag="VFE39_13845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HET6259725.1"
                     /translation="MTFDAQTFDAQTFDAQTFDALWADLEPVGRHPGTGGYRRFAWTR
                     EDAALREWFVGEAQRRELDVIPDRAGNLWAWWGDPDAYGPGLVVGSHLDSVPDGGAFD
                     GPLGVVSALAALDALRARGWTPRSPLGIAMFSDEEGARFGVACAGSRIITGALDADRA
                     RSLTDVDGTTMAEAMAAAGHDPGQVGPDKEALRRVGTFVELHVEQGKGLVDAREGEGS
                     AVGVASSIWPHGRWRLDLHGRADHAGTTRLTDRDDPMLALAAVLATARTAAQRHAALA
                     TVGKLRVEPNGVNAIPSAVTAWLDARGPVERDVRSVVAEVAAAAGTDPVEESWTPPTD
                     FDTVLRDRIAALLCDAGGPAPVLPTGAGHDAGILSAAGVPTAMLFVRNPTGVSHSPHE
                     HAEFADCHRGVAALAGVMEELA"
     gene            complement(9287..9535)
                     /locus_tag="VFE39_13850"
     CDS             complement(9287..9535)
                     /locus_tag="VFE39_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259726.1"
                     /translation="MTEEEPILSRVERRSTDLVLDAERHGSPRSQFTLWFGANVQVTV
                     TSSTRAARVSRAGRAVFIAPNAPAEMIYPAEALAAGVR"
     gene            complement(9532..11211)
                     /gene="hutU"
                     /locus_tag="VFE39_13855"
     CDS             complement(9532..11211)
                     /gene="hutU"
                     /locus_tag="VFE39_13855"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HET6259727.1"
                     /translation="MEGARPVRAARGSTLTARSWQTEAPLRMLCNNLDPEVAERPDDL
                     VVYGGTGRAARDWVSFDAMVRTLTTLAADETMLVQSGRPVGVLRTHEWAPRVLIANSN
                     LVGDWATWPEFRRLERLGLTMYGQMTAGSWIYIGTQGILQGTYETFAAVAAKRFGGSL
                     TGTLTVTGGCGGMGGAQPLAVTLNGGVCLVVDVDAARLRRRVEHRYLDELGTDLDDAV
                     ARCEQARRERRAWSVGVVGNCAAVLPELLRRGVAVDVVTDQTSAHDPLSYLPDGVDLD
                     DWPAYAAAKPEEFTDRARESMARHVEAMVGFLDAGAEVFDYGNSLRDEARQGGYDRAF
                     AFPGFVPAYIRPLFCEGKGPFRWAALSGDPADIHATDDAVLELFPDDDRLHRWIRAAR
                     ERVAFQGLPARICWLGQGERDRAGLRFNEMVASGALSAPVVIGRDHLDTGSVASPYRE
                     TEAMADGSDAIADWPLLNALVNTASGASWVSIHHGGGVGIGRSLHAGQVTVADGTELA
                     ARKIERVLTNDPASGVLRHVDAGYEVASEVAAAHGLRVPMAERAATEEDQV"
     gene            complement(11299..12837)
                     /gene="hutH"
                     /locus_tag="VFE39_13860"
     CDS             complement(11299..12837)
                     /gene="hutH"
                     /locus_tag="VFE39_13860"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HET6259728.1"
                     /translation="MHAVVVGSDPLTTEEIVAVAREGAGVVLCPDAENEIEASRKVVE
                     ALADDVVPHYGVSTGFGALAARHIPRERRQQLQRSLVRSHAAGSGPEVEREVVRALLL
                     LRLSTLASGRTGVRPATVRAYAGMLAAGITPSVREYGSLGCSGDLAPLAQCALAVMGE
                     GEVRDAVGVLRPAGQALAAAGIEPVVLAEKEGLSLINGTDGMLGMLVLACHDLRALVR
                     TADVAAAMSVEALLGTDAVFAADLQALRPHPGQADSAANLRALLTGSAIMASHRTPDC
                     MRVQDAYSLRCAPQVHGAVRDTIAHAEMVAGRELASAVDNPVVTVDGRVESNGNFHGA
                     PLGYVLDFLAIAVADLASISERRTDRFLDVARNHGLPPFLADDPGVDSGHMIAQYTQA
                     GIVSELKRLAVPASVDSIPSSAMQEDHVSMGWSAARKLRRGVDGLARVVAIELLTAAR
                     ALDLRAPLAPAPATAAVRDAVRRHVAGPGPDRYLAPEIEAAVQLVTTGEALAAAEAVT
                     GPLR"
     gene            12927..13709
                     /locus_tag="VFE39_13865"
     CDS             12927..13709
                     /locus_tag="VFE39_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET6259729.1"
                     /translation="MPGPVKVPAAQQVLAILGYLARQAGPVPAAAITRDLELPRSTTY
                     QLLTTLAESGFVVHLADERRYSLGVAAYELGTGYVRQAPLQRIARVPLGRLVDRTGHS
                     AHLAVLHGREVVYVLEERAPGRPPLVTDVGVRLPAHLTASGRVMLAALPAAQIRALFP
                     QPDAFVTRHDVGPRSLSALRRELVETRRRGHAVEDGEVTPGFVSIAVGVLDHTRHPVA
                     SVAVTFPSYAEPGGHGALVDEVGRTARAIASRIGGHVRPAAG"
     gene            complement(13882..15519)
                     /locus_tag="VFE39_13870"
     CDS             complement(13882..15519)
                     /locus_tag="VFE39_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259730.1"
                     /translation="MTGPTATGGVAGTAAKETLEDYTLRFAPRSYRKWSSGVVAVSAL
                     GGIAYLADFSIGANIGISYGTTNALWGILVFAVVIIATGFPLAFYAARYNLDLDLITR
                     GSGFGYYGSVVTNVVFATFTFIFFALEGSIMAQGLQLGLNIPLWLGYALSTLVIFPLV
                     VYGMSLLSKLQLWTTPLWLVLMVLPFAYLLISHPESVSSFFAYRGQNGQGAPSLGSVM
                     LAAGVCLSLIAQIAEQIDYLRFMPPKTPENSRRWWAATILAGPGWVIFGAAKQVVGLF
                     LAVYIIANVADGATVANEPVHQFLEIYQDIMPPWAALTLAVILVVLSQVKINVTNAYS
                     GSLAWTNSYTRVTKHYPGRLVFLGVNLLIALVLMEANMFEFLNTILGFYANCGMAWVV
                     VVASDIAINKYLLKISPKKPEFRRGMLYAFNPVGFGSMLIAAGVSVVAFFGGLGSGIQ
                     PFSPLVAIGLGLVLPPIIALATKGKYYLRRTDDGIDLPMYDEHGNPSGHVLRCHVCRQ
                     EFERPDMVACGTHDAQVCSLCLSTDKVSDHVLPPLSG"
     gene            complement(15632..16990)
                     /locus_tag="VFE39_13875"
     CDS             complement(15632..16990)
                     /locus_tag="VFE39_13875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259731.1"
                     /translation="MTRRPANRLDAQDVVASFDSATVLHAALSAALRGEPFPHLGNGP
                     LAGAAIRAAGRLPWPLLRRLYTRIGASEGVDPGRLGDVDMNAVAGWLADGYPQRCYPA
                     ALVGSSNGALAHLAAAMQVPWLPGTVLVPVARTGDPQRPVDALHFGERAAPALLDRNP
                     DVVLHHMHDQVQDELMVARMTYFRIKWQTLPEPYARFLTGFLAPGAPVVLVEDRSRWP
                     VVRVGDRHVFQTGAQGGLEPQAYLDRPHTPRPDGEEPEAEWGADPQFGTSLAGWCVAH
                     GHPLLRIAYSGPQAPAHAVASVVRDWYTRRGEPADRLVVPSFVLGDPWRTLNAAAVPF
                     WTFFSVQPALRALEDHLLASAPYRNVDILLFQHGVHSAGIARPEEWVRVARGNGATAR
                     LMAVSPRRFPHDIASLGRYGRMLAELPGAHLTWSPLEAARAVRELNARLDDPGPTHGQ
                     LA"
     gene            complement(16987..17631)
                     /locus_tag="VFE39_13880"
     CDS             complement(16987..17631)
                     /locus_tag="VFE39_13880"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6259732.1"
                     /translation="MRVLIVPGAAVRQYVRPAVEAVASRGIDARLLPAPGEPGAPADL
                     QNYGEELARRISTGSPLDLLVGLSVGAQAAAVAATAVPTGQVRHVMLISPTVDPKVRT
                     APRLLGRWLAGGRLERPGLLSEQAPDWRRAGLRRLVRVVRSALAVRIEDLLPRLGAAL
                     TVGHAEHDVITSHSYAARLAADHRGRFIVVPGATHSWPYADADRFADTVEDVLG"
     gene            complement(17791..19929)
                     /locus_tag="VFE39_13885"
     CDS             complement(17791..19929)
                     /locus_tag="VFE39_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET6259733.1"
                     /translation="MSIIVVSDAAGRIRFATPELTGSSALAVAVEDAVDAVPHVRQVH
                     AYPRTGNVVVWFDPQRCDRTELVEAIGKGLGADPQATAERTPRSADVSNGDLVRLVVG
                     GVALAGLGLRRYALRRPPMLGPSSRTVATGVTIFTGYPFLRGALRSLGRGRGAGTDAL
                     VSAATVASLVLRENVVALTVLWLLNIGEYLQDLTVRRSRRAISELLTGAQTRAWTRLS
                     DGAELEVDIDQLAIGDDVVVHEHVVLPVDGVVVEGDGVVDQAAITGEQLPVSVTVGTP
                     LHAGTVLLRGRLVVRASAVGRDTAIGRIIHRVEQAQRDRAPIQTVGETFSRRFVPTSF
                     ALSALTLLVTRDVRRAMTMLLVACPCAVGLSTPTAISAAIGNGARRGILIKGGAHLEA
                     AGRMDAIVFDKTGTLTTGRPVVTNVVSFDENWSPEQVLAYAASSEIHSRHPLAQAVIR
                     STEERHITIPPHEECEVLVGQGMRVESDGRILVIGSRELLRQQQIRVSREAHVWVRRL
                     QRATETPLLLAVDGTLVGLVSLRDTVRPEARQVLDALRADGVRRVVLLTGDHPETAAA
                     VAAELGIEEFWAQVPPEDKQDLVRSLQAEGHTVAMVGDGTNDAPALALADIGIAMGVS
                     GTDVAVETADVALAGDDLRRLLDLRDLGRRSIGVIRQNYGMSIAVNAAGLLVSAAGAL
                     SPVVAAILHNASSVAVVGNSSRMIRYRLEP"
     gene            complement(19937..20212)
                     /locus_tag="VFE39_13890"
     CDS             complement(19937..20212)
                     /locus_tag="VFE39_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1490 family protein"
                     /protein_id="HET6259734.1"
                     /translation="MIGGFVGRAAGLVASGVAGAVAYDGIKRLARSNVLHGAAVTVTS
                     CALRGARVAETGAEKARLTTADIVSEARERIGEQTPAPGAAVPAHEH"
     gene            20532..21023
                     /locus_tag="VFE39_13895"
     CDS             20532..21023
                     /locus_tag="VFE39_13895"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6259735.1"
                     /translation="MTSPRTPSTADAVSEALMPAVGALRRLLRRAAGPVFTSDTVSTS
                     QREVLVAVGRHPGSPVTDIAQELGLAPNSVSTIVTQLVGAGLLVRETDPHDRRVGRLT
                     LTPRAQEQAVVARARRRTVLHDALDRLTPRQVVELAAGIDALGALADELRALERDREA
                     VHP"
     gene            21095..21898
                     /locus_tag="VFE39_13900"
     CDS             21095..21898
                     /locus_tag="VFE39_13900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6259736.1"
                     /translation="MLALECSGLVHRFGDTVAVDHLDLRIQAGEVFGLLGPNGAGKTT
                     TIRVLNTLLPVQEGSVRVFGLDVRRRATDVRRQLGYVPQQLSIEAALTGRQNVMWFAR
                     LFDVPRAERRARVDEALAAMDLLAVADRRAGTYSGGMIRRLELAQALVNHPALLVLDE
                     PTVGLDPIARAGVWERVRQMRADYGMTVLLTTHYMDEADELCDVVALMHRGRLQVVGS
                     PAALRADIGPDATLDDVFRRYAGTKLVATEEQGGLRDVRRSRTTARKLG"
     gene            21858..22664
                     /locus_tag="VFE39_13905"
     CDS             21858..22664
                     /locus_tag="VFE39_13905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6259737.1"
                     /translation="MSVAVARPRANWAEPVRMLASRTVTLSLVELQKLRSDYTELITR
                     MAQPVLWLLVFGQIFNRIRAIPTGNLSYLAYLTPGILAQSALFVAIFLGIQIIWDRDA
                     GILTKVMVTPTPRAAIVLAKAFAAGVRGLAPVVVLVPVALLLGVAITINPLKLLGAAV
                     ALMLGAGFFACLSMAIAGVAQTRDRLMGIGQLMTMPLFFASNALYPIDIMPGWLRVIS
                     QVNPLTYLVNALRGLLVGAEANLWVDFGVLVGAAVVGIVGAASLLGKLVR"
     gene            22775..23221
                     /locus_tag="VFE39_13910"
     CDS             22775..23221
                     /locus_tag="VFE39_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET6259738.1"
                     /translation="MPMPFPARVLRQLPGRFAVCRFDPNEPVPRWALDGPVEFVSVTR
                     TRAELSVICPVEVLPDPDLAGLEWHCLQIEGPFDFDELGVLASVVDPLAQAGVSVFAV
                     ATYDTDYLLVTDIDGARRALLAAGHEIRPAVTGAWTRPSASGGEHR"
     gene            23218..>23666
                     /locus_tag="VFE39_13915"
     CDS             23218..>23666
                     /locus_tag="VFE39_13915"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HET6259739.1"
                     /translation="MSTDNPWVVRRRVLPAPRLRMFCFPYAGGGASVYRDWPRWLPPD
                     LEVCAVQPPGRESRLAEPPYTRLPDLLPPLADALSGLLDGPFVTFGHSLGGLVSFELV
                     RYLRRAGGPQPEQMYVSGRRPPHLPGRERPIHDLPDDDLLTAVLRLNG"
BASE COUNT         3394 a         8622 c         8384 g         3266 t
ORIGIN      
        1 ggcgggggag atcgcggtac tggccaaggt gcaccacgcc ctgggcgacg gcctcgccgt
       61 caccgacgcg ctgatccggt tgctgaccga cgaggcgccg ccccccgtgg ggccgcccgc
      121 gcaggaccgg gaccctccca ccggggcggc cgaccggctg cgccgcgctg ccaccgtggt
      181 cagggggatc accagcctcg ccatcgccgg gggcgccggg acatccccgc tcgccgggcc
      241 ggtcacgagc gccgcgcacc gccgcgccgg cgtgtgcttc gacggcgcgc aggtccgggc
      301 ggcggcgcgg gcccacgggg tgggcaccac cgtgctgctg ctggccgtgg tggccgagac
      361 gctgcacagc ctgctcgccg agcgcgaccc ggacgccgtg cccgcgacgg ttcgcaccat
      421 ggtgccgatc accacccgga ccagtgcaac ggtccgcagc cgcgcgctgg gcaaccgcac
      481 cgcggccgtc tcgatcgacc tcccggtcgg cccgatgtcc cccgctcagc gcgtcgcgct
      541 ggtggggaca gcactcgctg ccggctcctc ggccggccag cccgacggtg cagcagcggt
      601 gctgcgcatt ctcgggctgc tgcccaagca ggtgcaggcc ctgctggtgc gccccatcta
      661 cgggcatcgg ttcttccatg ccctggcctc ggtcatgccg ggatcccggc gcccgctgca
      721 ctcccgcggc gggcttattc gcgaggttta tcccgttctg ccgctggccg atcgagtggg
      781 cctcgccgtc ggcgcactcc actggggccg caccacagcg atcggcatca cggcagatcc
      841 cggcctcgta cccgagatcg ggggaattcc cgatcgcgtc aaggcgtcgt taagcctgat
      901 gacgtaactg ggacctccac ggggtcacgg aggccgtaac agggttgcgg cgggctcaca
      961 gttgcggcga ctgttctcga tgcgggttgg aggtggcgat ggatacgggg acggcgctgg
     1021 tgatcgcggt gcctgctgcc gtgctcggtg ccgccggatt cgggttggcc agcgccgccc
     1081 agcaacgggc gaccaaggag gtggcggtca ccccgaccct gagcccgcgg ctgatcgtcg
     1141 agctgttggc gcggccgatg tggttgctcg ggttggtggc caccatcgtc gccctcgccc
     1201 tgcagatcgt cgcgctggcc ttcggtccgc tcgccgtggt gcaaccggtg ctcgtcaccg
     1261 gggtggcgtt cgccgcagtg ttctcggcgc tgatgcaagg ccgacacccc gaccctctga
     1321 tcctgctcgg tgcgctgtgc tgtgccgcag gcctctcggc gttcctcctg ctcgcccggc
     1381 ccaccgcggg cacgagcgac tacgggcagt tcgacctgct ggcgagtctg ccgttggcgg
     1441 tcgtcctgac cgccgtcgtt atcggctgcc tggtgtacgc cgctgccgtg cagcactcgt
     1501 cgcgggtgct ggcgctggcg ctggcgaccg gcgtgctcta cggggtcacc gccgggctga
     1561 tgaagatcat caccagccag ctgcgggtgg ggctggacga gcccttccgg cacccggtcc
     1621 tgtacgtcgt ctgcatcatc ggcccgatcg ggttcctgct gagccagaac accttccagc
     1681 aaggacagct cgtggcaccg gccgtggccg tgatcaccag cgtcgatccg gtggtcggcg
     1741 tcctcatcgg cgtgggctgg ttgggcgagc agatcgacag ctctccggcg ctgctcgccg
     1801 gcgagtgtct cgcggccctc gtcgtcatcg gctcgatcgc gctcatcacc gcacgtggtt
     1861 cctggctgct ccaacaccgg ctcgagctca cgccgcagtc cgctctcatg ccccgccgcc
     1921 gtgcgggccg ccccatctcc ggatgagacc gctaccgcgc aacgcggtgg tgaccggcgc
     1981 ctcgtccggg atcggtgcgg ctgtggcgca gcggctcgct cacgacgggt gcacgctgct
     2041 gctggtgggc cgcgaccgcg cacgactcga cgccgtcgcc gcccgcaccg gggggcgctc
     2101 cgtgctggcc gacctgcggg agcccgcagg tatcgaggct gtgtgcgatg ccgccgacgg
     2161 ctgggtggag ctgctggttc acggcgccgg cgtcggcgcc gccggaccgc tggccgacac
     2221 cccttccgac gtcatcgacg agttgctggc ggtcaacctc gtcgctcccg tccagctcac
     2281 ccgcgccctc ctaccgacgc tgcgcgccgc ctgcggccac gtggtgttcg tcgcctccgt
     2341 cgcggcgatc ggggtggcgc acgaggccgc ctactccgcg accaaggcgg gcctgcgtgg
     2401 gttcgcggac gcactccgcg tcgagtcgga catcggtgtc accacggtgc tgcccggcgc
     2461 tgtcgagacc ccgtactacg agcgacgcgg gcagccctac caccggcgct tcccccgccc
     2521 gatcggcccg gagcgggtgg ccgcggccct cgtcgacgcg gtacgccgga accgggcaga
     2581 ggtgttcgtg ccgcgctggc tcacagtggg cgcgcgggtg cacggggcgg cacccgcgct
     2641 cttcgcgcac ctgtcacgcc gcatggaccg ccgcgagcag ctgagctgat cggatcggct
     2701 gacaacccac tgggcgttgc cgacgtcctg gccgacggcc cgagtagtac gcacgacatc
     2761 gagctccctt cgagcagcgc gcccgacatc cgtactggtg cacgccaccg ccatcctgag
     2821 ggcggccacg tcgaactcgt gcagatcatc gagcccggat ccgggtctgg cggtcctgga
     2881 gaccgcgccg gtggctggct gaccggaccc gcggacgggt ggcgcccgag gcttcggtgt
     2941 ggggtccgtc gaggtggcgt cagagccgcc gtgagcgcgg ccgcgctcag ggccaggcag
     3001 agccaagcga accagtctcc ggcccgggag tagggcgtgc cgcctcccgc cgacgggagg
     3061 tcggctgtga ccgaagcggc ccggataccc gccgtgctcg ccgcctgggc gagcacccgc
     3121 ccgttgctgt cgctcagggt gaggtacccg tgccgggcgg cccgcgccac ggagaacccg
     3181 ttctccactc cgcgcagcag ctgcacgcgg ctctgcgccc acgcgtccac gtggaagtcg
     3241 agggcgggca cggccttcag gccgacgccc gcgcggccgt actcccggcc gagcgacacc
     3301 tgtcccatgt cagcgcagat cgccactccg ccccggccgt cgccggggag gaaaccgggg
     3361 gcaccacccg gcgtgagggc atcctccaca cccggtaccg ggtaccgctt gtggtagacg
     3421 aggggggccg agccgtcccg cgggaaggtc agcgcggtgt tgtagaggtg tgcgccggaa
     3481 cggtgctcca cgccgagtac caggacgacg tcccgttccc gcgccacggc cgcgaaccga
     3541 gcgacgacac cgggcagcgt ggcgtcctcg acgtggatgt ccttctccgg gaacaccacc
     3601 acctgtccgg gggtcgcctc agcgagcacg agcgcaccca ccgctcccgc ccgtcggcgt
     3661 gctgtccccg tcgacgacgg agcggtgagc gcggcgagtg ccgccgtcgt ggtgaacagc
     3721 acgaacgcca caccccaagg accggtgaac gagacgacct ggagtaccgg aaggttgtcc
     3781 gcctggctgt tggcgatcgt ccagttcgag ccggtcgggg tgagcacggc cacgaggtac
     3841 tccgccgccg cccacgtcgc cccggcgccg agcaccgcga ggagggtgtg accgcgccgg
     3901 accacggccc ggaactccag tgccacgacg gtgaacacgg cgcgtagtgg gcgtagccgg
     3961 tcatcccgag cagctgcgcc agtgcggcga cagacccggt ggtgcgcgcc gagactcgtg
     4021 gtgcgaacag gagcaccgga agcggggcga gccaggtgac ccaccagacg gggtgcaggc
     4081 cggtaccgac gaagaacagg gcggccgagg cgatcagggc cacggccacc ggcaccgccc
     4141 gggccgcggg gtgctcagcg acggacgcca tcgaggatcc tctcgacggc gcggctgcac
     4201 agcgctcgaa actcgctctc ggccaacccg atccgacctc cgtggtacat ctcgaccagg
     4261 ccgtgcagct gtgcggcgat gctcagcgcg acctcccaca cgtcgtgcgg aacgaacacg
     4321 ccgcgccgaa cgccggccgt cagcgcatcc gccacgggag tgagcgtggg cgaggcgccg
     4381 gagcggaaat cgtgcgggaa ggcgcgagcg cccgtgcggt tctcggtgaa caggaagacg
     4441 tagaggtgcg gctggcccag cgcgaagtcc aggtgctcgg tcagcaagcg ttccagcgct
     4501 ccgtcgtcgt caccggacca tgggtggccg ccccagccct cggccagttc ggcgaagcac
     4561 gcgtcggcga ccgctgcgag cagcgcgtcc cgactggggt agtgccggta ggtcgccatc
     4621 tggctgagcc ccacggattt ccccacccgc cgcatgctga cagccgccga gccctcctcc
     4681 acgacgatcc ggcgggcggc ctcggcgatg cgttccctgg tcgacacgtt tacaccgtaa
     4741 accacgccgt cagaattcgc gcactcaact ggggtcctga ccggcgttcg acccaaggtt
     4801 catctcgagc cggccgtgcc cggactggtc agggctgggt gtggaaggcg aagccgtcct
     4861 tggagcaacc gatcgttccg gtgccgaacg cgccggtgtc ggccaggcac gtccacccct
     4921 gcaccacgag gcggtgggcg gtgccttccg acatcgtcgg ggcatccctg tagtagtccg
     4981 agatgatgtt gatcgcttcg gtgcacccga ccgtgcctgc cgcggtgcca tcggcgatca
     5041 gatcgacctc ggcgccgccg gtggggccga ccttgccaca gttcacggcc tccaggtcgg
     5101 tgccggcgtc ggatctgttc ccgcccgccg gttgctgggc cgacggtgtc gacgacggtt
     5161 cggtcgtggc caccgtgggg gcgccggcga ccgttgacga aatggcggcg ggcgcagccg
     5221 acgtggccgg gtgggacgag catgcggcag cgagtccgac tgcggcgagc accaggccga
     5281 cggtcaacgg tcgaatcgtg cggtcatgca cctgtggact ccttagtgat cactcagaac
     5341 gggctgcatc gccggggtcc gtaccgcctc ggtgcatccg ggcgatccct gaccgagatc
     5401 gggtaggccg ctcggcgtgc tcgtcatcgg cgcgccacct cagctgatca acggaaccta
     5461 gcatcgggct cgagcggcga tcccggcgct cggctcacgc tcccggtttc gagcgtccgg
     5521 acgatcggca ctccgggccg gtaggccagg taccggtagg acggcgcgtc caggacggcc
     5581 aggtcagcgc gggcgcccac gccgaggcgc ccgacgtccg tgcggcgcag cgccgctgcg
     5641 ccaccggcag tggccgccag gacggcctcg accggcgtca tccgcatctc ccgcaccgcc
     5701 agcgccacgc acagggacat cgacgacgag aagcacgtgc ccgggttgca gtcggtcgcg
     5761 agtgccacgg tcacccccgc atcgagcagt cgcctgccgt ccgggtaggg gtgccgggta
     5821 gagaactcga ccccggggag cagtgtcgca accgtgtcgg cggacgcgag ggcgtcgacg
     5881 tcggcgtcgg tgagataggt gcagtggtcc acgctggcgg cgcccagctc gacggcgagc
     5941 tgcacgcccg ggccggcgcc cagctggttg ccgtgcaccc gcaggccgag tcccgcgtcc
     6001 cggccggcct ccagcaccac ccggctcgcg gcgccgtcga aggcgtgcgg tgacgcgggt
     6061 tcgcagaaca cgtcgatcca gcgcgcgtgg ggtgcgcagg cggccagcat cggccccgtg
     6121 accagcgcga cgtagtcgtc cacccggccc ccgtactccg ccggcaccac gtgcgccccg
     6181 aggaacgtcg tctcgtcggt gacctcccgg gcgagccgaa gggcacgcac ctcgtccgcc
     6241 accgtcaggc cataaccgct cttgacctcg acggtcgtcg tgccctgggc acgcatctcc
     6301 ccgacgagtg tgcgcaggcg cgaccgcagc tgctcctcgt ccgcctcgcg cgtggcccgc
     6361 acggtcgagg cgatcccacc gccgtcgtaa gggacgcccg acatcctggc ctcgaactcc
     6421 gccgaacgct cccccgcgaa gacgaggtgc gcgtggctgt cgacgaaccc cggcagcacc
     6481 gcccggcctc cgacgtccac ccgctggtcg gccgcgggtg ccgcggcggc cgggccgacc
     6541 caggcgaccg cgccgtcctg caccacgagg gccgcatcgg gcaccagccc cagcgggccc
     6601 gcgccgagcg acgggtcatt ggtgacgagc tcggcgaccc cggtgatcag cgtcgcgccg
     6661 tgagacgaac gccaggattc gatcatgggt tcaccgcgct ggtgttcatc tcatgctcca
     6721 gaagggctcg atcgcaccac gtagcagcgc gccgacatcg cccagccggt gcacgccccc
     6781 cgccacgacc ggcacgccgt cgaccagcac agtgtcgatg tccgccgcgg tggccaccag
     6841 caccacctgc tcaggggcgg caccagcggt gcgtggggtg tcgaggcgca ccgcgaccag
     6901 gtcggcccgc tgaccggggg cgatccggcc ggcgtccggc cagccgaggc aggcgtggcc
     6961 ctgcgcggtg agggcgtcga ccaacgcggc gggcctgaag cgaccccgtt tgccgctggt
     7021 cagccgctcg tgggcctcga cgagccgcgc ctcggcgagc agatcgctca tgacgtgctg
     7081 gtcgctgccg acggacaggg gcgacccggc gtcgcgcagg gcgcggaacg gtccgacgcc
     7141 gtcggcgaga tccgcctccg tgctggggca ggcgcacacg gtcgtgcccg atccgccgag
     7201 cagggcgatg tccgggagcg tcaggtgggt ggcgtgaacg gccgtggtgc tcggtccgag
     7261 gacgccgtgg tcggcgagca gcgcggcggg tgtccggccg tagaacgcct cgcacacctc
     7321 gttctcctgc ggttgctcgg agacgtgcac gtgcagcgcg cggccggcgg tggccgacgc
     7381 gacctgcggc agctgcgccg cggggaccgc ccggaccgag tggatggcgg cgccgatgcg
     7441 cagcgtgtcg ctgtcctcga gccggtcgac ccgggccgcc cagtgcgccg catcgccgtc
     7501 ggagaaccgg gtctgcacct cgtcgagcgg ggtgtggccg gccgcggtca ggccgcctgc
     7561 gaggtagcag gtgtccagaa gcgtcagccg cacgcccgca tcggcggccg cctggaccag
     7621 agcaccgccc atctcgttcg ggtcggcgta cggggtgccg cccgggccgt ggtgcaggta
     7681 gtggaactcc ccgacgaccg tgacgcccgc cagcgacatc tccgcgtacg cggcgcgggc
     7741 cagggcgagg taggagtccg ggtccagccg cgcggcgacg gcgtacatcc ggtcccgcca
     7801 cgtccagaag gtgccgcccc ggtcgtgggt ccgaccgcgc aacgcccggt ggaaggcgtg
     7861 gctgtgggcg ttggcgaaac cgggcaggac gagacccggc agccgctcgt cacctggtgc
     7921 tgggtccacc ccggtctcga cggcggcgat ccgggcgtcc cggacggcga cgcggacccg
     7981 gggaaccggg ccctgtccga gccacgcatg ctcgcaccag aacgcctgcc cctcgggctc
     8041 caccgtcacg ccagctcctc catgacgcct gcgagggccg cgacgccgcg gtggcagtcg
     8101 gcgaactcgg cgtgctcgtg cggtgagtgg gacacgccgg tcgggttgcg cacgaacagc
     8161 atcgcggtgg ggacgcctgc ggccgacagg atcccggcgt cgtgaccggc cccggtcggt
     8221 agcaccggcg ccggtccccc ggcgtcgcag agcagggcgg cgatgcggtc gcgcaggacc
     8281 gtgtcgaagt cggtcggcgg tgtccaggac tcctccacgg ggtcggtgcc cgcggccgca
     8341 gcgacctccg cgaccaccga ccgcacgtcc cgctccaccg gcccgcgcgc gtcgagccag
     8401 gcggtgacgg ccgacgggat ggcgttcacc ccgttcggtt ccacccggag cttgcccacc
     8461 gtggccaacg ccgcgtggcg ttgagccgca gtgcgcgcgg tagccaggac cgcggccagt
     8521 gcgagcatcg ggtcgtcccg atcggtcagc cgggtggtgc cggcgtggtc ggcgcggccg
     8581 tgcaggtcca gccgccaccg gccgtgcggc cagatcgagc tggcgacacc gaccgccgac
     8641 ccctcgccct cgcgcgcgtc gaccaggccc ttgccctgct cgacgtgcag ctcgacgaag
     8701 gtccccacgc gccggagcgc ctccttgtcg ggtccgacct gcccggggtc gtgtccggcc
     8761 gctgccatcg cctcggccat ggtcgtgccg tcgacgtcgg tgagcgaccg ggcacggtcg
     8821 gcgtccaggg cgccggtgat gatgcggctg ccggcacagg ccacgccgaa ccgcgcaccc
     8881 tcctcgtcgg agaacatcgc gatgcccagc ggactacggg gcgtccatcc cctggcgcgg
     8941 agcgcgtcca gcgccgcgag cgccgacacg acgccgagcg ggccgtcgaa cgccccgccg
     9001 tccggcacgg agtcgaggtg gctgccgacc accagccccg ggccgtaggc gtcgggatcg
     9061 ccccaccagg cccacaggtt gcccgcgcgg tcaggaatga catcgagctc ccgccgctgc
     9121 gcctcgccga cgaaccactc gcgcagcgcg gcgtcctccc gggtccaggc gaaccggcgg
     9181 tacccgcccg tgcccgggtg gcgcccgacc ggctccaggt cggcccacag ggcgtcgaag
     9241 gtctgggcgt cgaaggtctg ggcgtcgaag gtctgggcgt cgaaggtcat cgcacacctg
     9301 ccgcgagggc ttccgccggg tagatcatct cggccggagc gttcggcgcg atgaacacgg
     9361 cccgccccgc ccgtgacacc cgcgccgccc gcgtgctcga tgtgacagtg acctgcacgt
     9421 tggcgccgaa ccacagcgtg aactggctac gcggcgagcc gtgccgctcg gcgtcgagaa
     9481 ccaggtcggt ggaacgccgc tcgacgcgcg agaggatcgg ctcctcctcg gtcatacctg
     9541 gtcctcctcg gtagcggccc gctctgccat cggcacccgc agcccgtgcg cggcggccac
     9601 ctcggatgcc acctcgtacc cggcgtcgac gtggcgcagc accccgctgg ctgggtcgtt
     9661 ggtgaggacg cgctcgatct tccgtgcagc cagctcggtg ccgtcggcca cggtgacctg
     9721 cccggcgtgc agggagcgcc cgatcccgac cccgccgccg tggtggatcg acacccaact
     9781 cgctccggac gcggtgttga ccagggcgtt gagcagcggc cagtccgcga tcgcgtcgga
     9841 gccgtcggcc atcgcctcgg tctcccggta gggcgaggcc accgatcccg tgtcgagatg
     9901 gtcgcggccg atcacgacgg gtgcggacag ggctccggag gcgaccatct cgttgaaccg
     9961 cagcccggcg cggtcccgtt ccccctgccc gagccagcag atccgggccg gcagcccctg
    10021 gaacgccacc cgctcgcggg ccgcgcggat ccaccggtgc agccggtcgt catcggggaa
    10081 gagctccagc acggcgtcat cggtcgcgtg gatgtcggca ggatcgccgg agagcgccgc
    10141 ccaccggaac gggcccttgc cctcgcagaa caggggccgg atgtaggccg ggacgaaacc
    10201 ggggaaggcg aacgcgcggt cgtagccgcc ctggcgggcc tcgtcgcgca acgagttgcc
    10261 gtagtcgaac acctcggcgc ccgcatcgag gaacccgacc atggcctcga cgtggcgcgc
    10321 catcgactcg cgcgcccggt ccgtgaactc ctcgggcttc gcggccgcgt acgccggcca
    10381 gtcgtccagg tcgacgccgt ccggcagata ggacagtggg tcgtgcgcgg aggtctggtc
    10441 ggtgacgacg tccaccgcga cgccgcggcg cagcagctcc ggcagcactg cagcgcagtt
    10501 gccgaccacc ccgaccgacc acgcccggcg ctcccgcctg gcctgctcgc agcgcgccac
    10561 cgcgtcgtcg aggtcggtgc ccagctcgtc gaggtagcgg tgctccaccc gccggcgcag
    10621 ccgcgccgcg tcgacgtcga ccaccaggca gaccccgccg ttgagggtga cggcgagcgg
    10681 ctgcgcgcca cccatgccgc cgcagccgcc ggtgacggtg agcgtgcccg tcaacgagcc
    10741 accgaaccgc ttcgcggcga cggcggcgaa cgtctcgtac gtgccctgca ggatgccctg
    10801 ggtgccgatg tagatccacg agcccgcggt catctgcccg tacatggtga ggccgagccg
    10861 ctccagcctg cgaaactcgg gccaggtcgc ccagtcgccg acgaggttgg agttggcgat
    10921 cagcacgcgc ggcgcccact cgtgcgtgcg cagcaccccg accgggcgcc cggactgcac
    10981 gagcatcgtc tcgtcggccg ccagcgtggt gagcgtgcgc accatggcgt cgaaggacac
    11041 ccagtcccgc gcggcccgcc cggttccgcc gtagaccacg aggtcgtcag gccgctcggc
    11101 cacctccggg tcgaggttgt tgcagagcat ccgcagcggg gcctcggtct gccaggaccg
    11161 ggcggtcagg gtcgagcccc tggcggcgcg gacgggacgg gcgccttcca tgagttgcct
    11221 ccttcgtcgg gtcgtgcgtc agcgggtcgt gcgtcagcgg gtggtgggtc agcgggtcgt
    11281 gggtcagcgg gtcgtgggtc agcgcagtgg gccggtgact gcctcggcgg cggccagcgc
    11341 ctctccggtg gtgacgagct gcaccgccgc ctcgatctcg ggcgccaggt accggtcggg
    11401 gccgggaccg gcgacgtggc gccgcacagc gtcccggaca gcggcggttg ccggggccgg
    11461 ggcgagcgga gcccggagat ccagcgcgcg ggccgccgtg agcagctcga tcgcgacgac
    11521 ccgagccagc ccgtcgaccc cgcgacgcag cttgcgagct gccgaccagc ccatcgagac
    11581 gtggtcctcc tgcatcgcgc tcgacgggat cgagtcgacc gacgcgggca cggccagtcg
    11641 cttcagctcg gacacgatcc cggcctgggt gtactgcgca atcatgtgcc cggagtccac
    11701 cccggggtcg tcggccagaa acggtggcag cccgtggtta cgggccacgt cgaggaaccg
    11761 gtcggtccgc cgctcactga tcgaggcgag atcggcaacc gcgatcgcca ggaagtcgag
    11821 cacgtacccg aggggggcgc cgtggaagtt cccgttgctc tccacccgac cgtcgacggt
    11881 caccacggga ttgtcgaccg cgctcgccag ctcccgcccg gccaccatct ccgcatgagc
    11941 gatggtgtcc cgcaccgccc cgtgcacctg gggggcacag cgcagcgagt aggcgtcctg
    12001 caccctcatg cagtccggcg tccgatgact cgccatgatc gccgaaccgg tcagcagcgc
    12061 gcggaggttg gctgcgctgt ccgcctgccc cgggtgtgga cgcaacgcct gcagatcggc
    12121 ggcgaacacc gcgtcggtgc ccagcaacgc ctccacactc atcgccgcgg ccacgtcggc
    12181 ggtccgaacc aacgcgcgca ggtcgtgaca ggccagcacc agcatcccga gcatcccgtc
    12241 ggtgccgttg atcagcgaca ggccctcctt ctccgcgagg acgacgggct cgatccccgc
    12301 cgcggccaat gcctgccccg caggccgcag cacgcccacg gcgtcgcgga cctcaccctc
    12361 gcccatcacc gccaacgcac actgggcgag cggagccaga tcaccggaac accccaggga
    12421 gccgtactcg cgcaccgacg gcgtgatccc cgcagcgagc atccccgcgt acgcccgcac
    12481 cgtcgccggc cggacaccgg tacgcccgct cgccagcgtc gacagccgca gcagcagcag
    12541 cgcccgcacc acctcacgct ccacctccgg ccccgacccg gccgcgtgcg aacgcaccag
    12601 cgagcgttgc agctgctgcc gccgctcacg agggatgtgc cgcgcggcca gcgccccgaa
    12661 ccccgtcgac accccgtagt gcgggacgac gtcgtcggcc agcgcctcga ccaccttgcg
    12721 gctggcttcg atctcgttct cggcatccgg gcagaggacg acaccggcgc cctcccgggc
    12781 cacggcgacg atctcttcgg tggtgagcgg gtcggagccc acgacgactg catgcatcgg
    12841 ctcataggac accgcacccg ctcgcgtcca ccacggcgcg cggcatagag ctgtccggga
    12901 taccggacag ttgctagcgt cggcgggtgc ccggtccggt gaaggtgccc gcggcgcagc
    12961 aggtgctggc catcctcggc tacctcgcgc gccaggccgg tcccgtgccc gcggccgcga
    13021 tcacccgtga cctcgagcta ccccggtcca ccacctacca gctgctcacg acgctcgcgg
    13081 agagcgggtt cgtcgtccac ctggccgacg agcggcgcta cagcctcggc gtcgccgcgt
    13141 acgagctcgg caccggttac gtccgccagg cgccgctgca gcggatcgcg cgggtgccgc
    13201 tcggccgcct cgtggaccgg acggggcaca gcgcacacct ggccgttctc cacgggaggg
    13261 aggtcgtcta cgtcctcgag gagcgcgcgc ccggccggcc gcccctggtc accgacgtcg
    13321 gagtgcggct gcccgcccac ctcacggcca gcggccgggt gatgctcgcc gcgctacccg
    13381 cggcgcagat cagagccctg ttcccgcagc ccgacgcctt cgtcactcgg cacgacgtcg
    13441 gtccgcggtc gctgtccgcg ttgcgcaggg agctcgtgga gacccgtcgc cgcggtcacg
    13501 ccgtcgagga cggcgaggtc acccccggct tcgtctccat cgccgtggga gtcctcgacc
    13561 acacccgcca cccggtggcc agcgtggccg tcaccttccc ctcgtacgcg gaacccgggg
    13621 ggcacggcgc gctcgtcgac gaggtgggcc ggaccgcgcg ggcgatcgcc agccgaatag
    13681 gcggacatgt gcgccccgct gcgggctagc gctccatccg tagatcgaac aggaggggag
    13741 cgttgcccag ccgccgtcag cctcccgttc gcatccaccg accatgaccc gtccggcgag
    13801 gtggactcat cgcacggcgg tgaggaatcg cgggggcccg gcggcccggc cccgaccgca
    13861 gggccgggtc gccgcgctgc gtcagccgct caggggcggc agaacgtgat cgctgacctt
    13921 gtcggtactc agacacagcg agcacacctg ggcgtcgtga gtcccgcacg cgaccatgtc
    13981 gggccgctcg aactcctggc ggcagacgtg gcagcggagc acgtgaccgg acgggttgcc
    14041 gtgctcgtcg tacatcggca ggtcgatccc gtcgtcggta cgccgcaggt agtacttccc
    14101 cttcgtcgca agggcgatga tcggcggcag gaccagaccg agaccgattg cgacgagcgg
    14161 cgagaacggc tggatccccg atccgagccc gccgaagaag gcgacgacgg acacgccggc
    14221 cgcgatgagc atcgaaccga agccgaccgg gttgaacgcg tacagcatgc cgcgccggaa
    14281 ctccggcttc ttcggcgaga tcttcaacag gtacttgttg atcgcgatgt ccgaggccac
    14341 cacgaccacc caggccatac cgcagttcgc gtagaagccg agaattgtgt tcaggaactc
    14401 gaacatgttc gcttccatca gcaccagtgc gatcagcagg ttgacaccga ggaacaccag
    14461 ccggcccggg tagtgcttcg tgacgcgggt gtaggagttc gtccacgcca gcgatccgga
    14521 gtaggcgttc gtgacgttga tcttgacctg gctgagcacg accagtatca cggccagggt
    14581 cagtgcggcc cacggcggca tgatgtcctg gtagatctcc aggaactggt gcaccggctc
    14641 gttggccacc gtcgcgccgt cggcaacgtt cgcgatgatg tagaccgcga ggaagagccc
    14701 gacgacctgc ttggccgcgc cgaaaatcac ccaacccggg ccggccagga tggtggcggc
    14761 ccaccagcgc cgggagttct ccggcgtctt ggggggcatg aaccgcaggt agtcgatctg
    14821 ctcggcgatc tgcgcgatga gcgaaaggca gacaccggcg gcgagcatga cggagccgag
    14881 gctgggtgca ccctggccgt tctggccccg gtaggcgaag aaggagctca ccgactcggg
    14941 gtggctgatc agcaggtacg cgaagggcag caccatcagg accaaccaca ggggtgtcgt
    15001 ccagagctgc agcttcgaca gcagcgacat cccgtagacc acgagcggga agatgaccag
    15061 cgtggacagg gcgtacccga gccacagcgg gatgttcagg ccgagctgca ggccctgcgc
    15121 catgatcgag ccctcgagcg cgaagaagat gaacgtgaac gtcgcgaaga cgacattcgt
    15181 gaccacggat ccgtagtatc cgaaaccgct gccacgggtg atcagatcca ggtccagatt
    15241 gtaccgggcc gcgtagaacg cgagcggaaa tccggtggcg atgatgacga cagcgaagac
    15301 caggatgccc cacaaggcat tagtggtgcc gtaggagatc ccgatgttcg ctccgatgga
    15361 gaagtcggcc agatacgcga taccgccgag tgccgaaacg gccacaaccc cgctcgacca
    15421 cttgcggtag ctgcgtggag cgaaacgtag cgtgtagtcc tcgagcgtct ccttcgccgc
    15481 ggtaccggcg acaccgccgg tcgcagtggg cccggtcaca ataggcgata gatcagatga
    15541 cacacgcacc tccgagtatt tgtgcagcac tcctgaaggc gagtatcagt cggcgccgaa
    15601 cccgggcggt ttcctttcga taatggctgt gttacgccag ttgaccatgc gtcgggccgg
    15661 ggtcatcgag acgcgcgttc agttcgcgga ccgcgcgtgc ggcctcgagg ggtgaccagg
    15721 tcaggtgcgc ccccggcagc tctgcgagca tgcggccgta gcgcccgagc gaggcgatgt
    15781 cgtgcgggaa acggcgtggg ctcaccgcca tcaggcgggc cgtggccccg tttccccgtg
    15841 cgacccgcac ccactcctcg gggcgcgcga tgccggcgga gtgcacgccg tgctggaaga
    15901 gcaggatatc gacgttccgg tagggagcgc tcgccaacag atggtcctcg agtgcccgga
    15961 gagcgggctg cacggagaag aaggtccaga acggcacggc ggcagcattc agggtgcgcc
    16021 acgggtcgcc gaggacgaag gacgggacca ccagcctgtc ggccggctcg cccctccgtg
    16081 tgtaccagtc gcgaaccacc gaggcgacgg cgtgcgcggg cgcctgcggt ccggagtacg
    16141 cgatgcgcag cagcgggtgt ccatgtgcga cacaccagcc ggccagcgac gtgccgaact
    16201 gcgggtcggc accccactcg gcctcgggct cttcgccgtc cgggcgtggg gtgtgcggcc
    16261 ggtcgaggta ggcctgcggc tccagcccgc cctgggctcc ggtctggaac acgtgccggt
    16321 ctcccacacg gacgacaggc catcgcgacc ggtcctcgac caggaccacc ggggcgccgg
    16381 gtgccaaaaa cccggtcagg aaccgggcgt agggctccgg aagcgtctgc cacttgatgc
    16441 ggaagtaggt catgcgcgcg accatcagct cgtcctggac ctggtcgtgc atgtggtgca
    16501 ggacgacgtc ggggttgcgg tcgagtagag caggcgcggc gcgctcgccg aagtgcagcg
    16561 cgtcgacggg gcgctgcggg tcgccggtgc gggcgaccgg aaccaggacc gttccgggca
    16621 accagggcac ctgcatcgcg gccgccagat gggcgagtgc gccgttgctg gagccgacga
    16681 gcgcggcggg atagcagcgc tgcggatagc catcggccag ccacccggcg accgcgttca
    16741 tgtcgacatc gcccagccgg cccggatcga cgccctccga cgcgccgatc cgcgtgtaca
    16801 ggcggcgcag cagcggccag ggcagcctcc cggcggcccg gatcgccgcg ccggccagcg
    16861 ggccgttccc caggtgtggg aatggctcac cgcgcagcgc cgcggacagc gcagcgtgca
    16921 gcacggtcgc ggagtcgaag gaggcgacga cgtcctgcgc gtcaagccgg ttcgcgggac
    16981 gtcgggtcat ccgagcacgt cctcgacagt gtcggcgaag cggtcggcgt cggcgtaggg
    17041 ccaggagtgg gtggcaccgg gaacgacgat gaaccgaccc cggtggtcgg cggcgagcct
    17101 tgccgcgtag gagtgcgacg tgatcacgtc gtgctcggcg tgccctacgg tcaaggcggc
    17161 gccgagccgg ggcaggaggt cctcgatccg caccgccagt gcggagcgga ccacccggac
    17221 caggcgacgg agtccggccc gccgccagtc cggggcctgc tccgacaaca gcccggggcg
    17281 ttccagccgg ccaccggcca gccacctgcc gagcaggcgg ggcgccgttc gtaccttcgg
    17341 gtccacggtc gggctgatca gcatcacgtg ccgcacctgt cccgtgggca cggccgtcgc
    17401 ggcgaccgcc gcggcctgcg cgccgaccga cagcccgaca agcaggtcga ggggagagcc
    17461 ggtgctgatg cgacgggcaa gctcttcgcc gtagttttgc aggtcggcgg gggcaccggg
    17521 ctcgccggga gccgggagca gacgtgcgtc gatgccacgg gaagccacgg cctcgacggc
    17581 ggggcgtacg tactgacgaa cggcggcgcc gggaacgatc agcacgcgca tgcgggaagt
    17641 ctgaagcatc cgggcagcca ccgctcaccg gcgtccgcct gcaggatcgt ccggcgggcc
    17701 gtgaccggtt tcgccccgcg caatcatcaa ctgttcggcc ctttagcgcc gcggcgaggc
    17761 agcgaccgcg ggagtccgac ggccgagaga ctagggctcc aggcggtagc ggatcatccg
    17821 ggagctgttg cccaccacgg cgaccgacga ggcgttgtgc aggatcgccg ccaccaccgg
    17881 gctcaacgcg ccggcggccg agacgagcag gcctgccgcg ttgaccgcga tcgacatgcc
    17941 gtagttctgc cggatcaccc cgatgctgcg ccggccgagg tcgcgcaggt cgagcagcct
    18001 gcgcagatcg tcgcccgcca gcgcgacgtc ggcggtctcg accgcgacgt cggttcctga
    18061 aacccccatc gcgataccga tgtcggcgag tgccagcgcc ggggcgtcgt tggtgccgtc
    18121 gcccaccatc gcgaccgtgt gtccctcggc ctgcagcgac cggacgaggt cctgcttgtc
    18181 ctcgggagga acctgtgccc agaactcctc gattcccagc tcggcggcga cggcggccgc
    18241 ggtctccggg tggtcgcccg tgaggagcac gacccgccgc acgccgtccg cgcggagcgc
    18301 gtcgagcacc tggcgcgcct ccggacgcac cgtgtcgcgc aacgacacga gcccgaccag
    18361 cgtgccgtcc acagcgagca gcaacggggt ctcggtggcc cgctgcagcc tgcggaccca
    18421 gacgtgggcc tcgcggctca cccggatctg ctgttgacgc agcagctcgc ggctcccgat
    18481 gaccaggatc cggccgtcgg actccacccg catcccctgc ccgaccagca cctcgcactc
    18541 ctcgtgcggg gggatcgtga tgtggcgctc ctccgtggag cggatcacgg cctgggccag
    18601 cgggtgccgc gagtggatct cggagctcgc ggcatacgcg agcacctgct caggcgacca
    18661 gttctcgtcg aaggacacga cgttggtgac caccggacga cccgtcgtga gcgtgccggt
    18721 cttgtcgaag acaatcgcat ccatccggcc ggcggcctcc aggtgtgcgc ctcccttgat
    18781 caggattccg cgccgcgcgc cgttgccgat ggccgcactg atcgccgtcg gcgtcgacag
    18841 cccgacggcg cacgggcacg cgacgagcag catcgtcatc gcccgtcgaa cgtcgcgggt
    18901 gaccagcagc gtcaacgccg acaatgcgaa cgaggtgggc acgaaccggc gggagaacgt
    18961 ctcccccacg gtctggatcg gcgcccggtc ccgctgggcc tgctcgaccc ggtggatgat
    19021 ccggccgatc gccgtgtcgc ggccgaccgc cgacgcccgc acgacgagcc gtccgcgcag
    19081 cagaacggtg cccgcgtgca gcggcgtgcc gaccgtgacc gagacgggca gctgctcgcc
    19141 ggtgatcgcc gcctgatcaa ccacgccgtc gccctcgacg actaccccgt cgacggggag
    19201 caccacgtgc tcgtgcacga ccacgtcgtc gccgatcgca agctggtcga tgtccacctc
    19261 gagctcggcg ccgtcggaca accgggtcca ggccctggtc tgggcgccgg tgagcagctc
    19321 ggagatcgcg cgcctacttc ggcgcaccgt cagatcctgc aggtactcgc cgatgttgag
    19381 cagccacagc accgtgagcg cgacgacgtt ctcccgcagc acgaggctgg ccaccgtggc
    19441 ggcggagacg agcgcgtcgg tgccggctcc cctgccgcgc ccgagcgaac gcagcgcccc
    19501 gcgcaggaac gggtagccgg tgaagatcgt gactccggtg gccaccgtgc ggctgctggg
    19561 tccgagcatc gggggccggc gcaacgcgta tcgccgcaga cccagtccgg ccagcgccac
    19621 cccgcccacg accagccgaa cgagatctcc gttggacacg tcggcggagc ggggcgtccg
    19681 ctcggcagtg gcctgtgggt cggcgcccag ccccttgccg atcgcctcca cgagctccgt
    19741 ccggtcacag cgctgcgggt cgaaccagac gacgacgttg cccgtgcgcg gataagcgtg
    19801 cacctgccgc acgtggggca cggcgtcgac ggcgtcctcc accgccacgg cgagtgccga
    19861 actaccggtc agctcaggag tcgcgaaccg gatccgaccg gcggcgtccg agacgacaat
    19921 gatcgacacg cggtgcctaa tgctcgtggg ccgggacggc cgcgccgggc gcaggcgtct
    19981 gttcgccgat cctctccctg gcttcgctga cgatgtccgc cgtcgtcagc cgggccttct
    20041 cggcccccgt ctcggccacg cgcgccccgc gtagggcgca ggatgtgacg gtgacggcgg
    20101 cgccgtggag cacgttgctc cgcgccagcc tcttgatccc gtcgtacgcg actgcgccgg
    20161 ccacgccgct ggccaccagt cctgccgccc tacccacgaa accgccgatc atctcaccag
    20221 ctccttgtcg agacgaaatc aaggaccgtg cgtgcggacc tgacctccga cccgggctgt
    20281 gcgtcgaccg gggtacctcg ccggggtggt ccactgcagg gcgagcatgc aacgaacagg
    20341 ccccgctcag cgtccagact aggggaacac cgcgtcgccg acctcccacg cgaatgacga
    20401 cggtcatcac agccgtggtg gtccgcgctc gcgggcctac cggcgcagac ctgggcaagc
    20461 gccatcgtcc tctgctacac cagatgcatc tgagtcagat tgactctgta ctagaggtat
    20521 atgctcggcc cgtgacttct cctcgcacgc catcgacggc cgatgccgtg tcggaggccc
    20581 tgatgcctgc cgtcggtgcg ctgcgccggc tgctgcgccg ggccgcgggg ccggtgttca
    20641 cgagcgacac cgtgtcgacg tctcagcgcg aggtcctggt ggccgtcggc aggcaccccg
    20701 gcagccccgt cacggacatc gcccaggagc tcgggctggc acccaacagc gtctcgacga
    20761 tcgtcacgca gctcgtcggg gcaggcctgc tggtccgcga gaccgacccg cacgaccgcc
    20821 gcgtcggccg gctcaccctc accccgcggg cacaggagca ggccgtcgtc gccagggcgc
    20881 gcaggcgcac cgtcctgcac gacgcgctcg accggctcac cccacggcag gtcgtcgaac
    20941 tggccgcggg catcgacgcg ctgggagcgc tggccgacga gctgcgagcg ctcgagcgcg
    21001 accgcgaggc ggtgcacccg tgagcgagct tgcgagcgaa tcaatggcgc agcacgagca
    21061 gagatctgga gtgctacaga acgcccgcga cccgatgctc gcgctcgagt gcagcggcct
    21121 cgtgcaccgg ttcggcgaca ccgtcgccgt cgatcacctc gacctacgga tccaggcggg
    21181 cgaggtgttc ggcctgctcg gcccaaacgg ggcaggcaag accacgacga tccgcgtcct
    21241 gaacacgctg ctccccgtgc aggagggcag cgtccgggtg ttcgggctcg acgtccgccg
    21301 ccgggccacg gacgtgcgcc gccaactcgg ctacgtgccc cagcagctct ccatcgaggc
    21361 cgcgctgacc ggccggcaga acgtcatgtg gttcgcccgg ttgttcgacg ttccgcgcgc
    21421 cgagcggagg gcgcgggtcg acgaggcact cgccgcgatg gacctgctcg cggtggcgga
    21481 ccgccgcgcg ggcacctact ccggcggcat gatccggcgg ctggagctgg cgcaggcgct
    21541 ggtgaaccac cccgcgctgc tcgtgctcga cgagccgacc gtcggcctcg acccgatcgc
    21601 gcgggcgggc gtgtgggagc gcgtgcgaca gatgcgagcc gactacggga tgaccgtgct
    21661 gctgaccacg cactacatgg acgaagccga cgagctgtgc gatgtcgtgg cgctcatgca
    21721 ccgcggcagg ctgcaggtcg tcggctcccc ggccgccctg cgggccgaca tcgggccaga
    21781 cgccaccctg gacgacgtct tccgccgata cgccggcacg aaactggtgg cgacagagga
    21841 gcagggaggg ttgagggatg tccgtcgcag tcgcacgacc gcgcgcaaac tgggctgaac
    21901 cggtccgcat gctcgcttca cggacggtga ccctcagcct ggtcgagctg cagaagctcc
    21961 gcagcgacta caccgagctg atcacgcgca tggcgcagcc agtgctgtgg ctactggtgt
    22021 tcgggcagat cttcaaccgg atccgggcga tcccgaccgg taacctgtcc tacctggcgt
    22081 acctgacccc gggcatcctc gcccagtcgg cgctgttcgt cgcgatcttc ctcggcatcc
    22141 agatcatctg ggaccgggac gcgggcatcc tcaccaaggt catggtcacg cccacgccgc
    22201 gtgccgcgat cgtgctggcc aaggcgttcg ccgcaggggt gcgcgggctc gcgcccgtcg
    22261 tcgttctcgt gccggtggcg ctgctgctcg gcgtggccat cacgatcaac cccctcaagc
    22321 tgctcggcgc tgcggtcgcc ctcatgctcg gcgccggctt cttcgcctgc ctctcgatgg
    22381 cgatcgcggg cgttgcgcag acccgcgacc gtctgatggg catcggccag ctcatgacga
    22441 tgccgctctt cttcgcgtcg aacgcgctct acccgatcga catcatgccg ggctggctcc
    22501 gggtgatcag ccaggtcaat ccgctcacct acctggtcaa cgccctgcgc gggctgctcg
    22561 tcggagcgga ggcgaacctc tgggtggatt tcggcgtgct cgtcggcgcg gccgtggtcg
    22621 gaatcgtggg ggcggcgtcg ctcctgggca agctggtgcg ctgagcccgc cgacctccct
    22681 gctcgcgctc gagggagaag tgagcaccgg tttccgcaag tccgcgccgg ccggttgagc
    22741 tgtaagtccc tgggtcatcc actagaacgg gctgatgccg atgccgttcc ctgccagggt
    22801 cctccgccag ctgcccggcc ggttcgccgt ctgccggttc gacccgaacg aacccgtccc
    22861 acgctgggca ctggacgggc cggtggagtt cgtctcggtg acccggaccc gcgccgagct
    22921 ctccgtcatc tgccccgtgg aggtgctgcc ggatcccgac ctcgccggcc tcgagtggca
    22981 ctgcctgcag atcgaggggc ccttcgactt cgacgagctt ggcgtgctcg cgtcggtggt
    23041 cgacccgctg gcacaggcgg gcgtgagcgt gttcgcggtg gccacgtacg acaccgacta
    23101 cctgctggtg acagacatcg acggggcgag gcgagcgctg cttgccgcgg gccacgagat
    23161 ccgccccgcg gttaccggtg cctggacccg gccgagcgca agcgggggcg agcacaggtg
    23221 agcaccgaca acccgtgggt ggtccggcgc cgggtactgc cggcgccgcg gctgcgcatg
    23281 ttctgcttcc cctatgccgg tggcggtgcg tccgtctatc gggactggcc acggtggttg
    23341 ccgccggacc tcgaggtgtg cgccgtgcag ccgcccggcc gggagtcccg gctggccgaa
    23401 ccgccctaca cccgcctgcc cgacctgctg ccgcccctcg cggacgcgct gagcggactg
    23461 ctggacggac cgttcgtgac cttcggtcac agccttgggg ggctggtcag ctttgagctg
    23521 gtcaggtacc tgcggcgcgc cggtgggccg cagccggaac agatgtacgt gtccggtcga
    23581 cggcccccgc acctgcccgg ccgggaacgg ccgatccacg acctgcccga cgacgacctc
    23641 ctgacggccg tcctgcggct gaacgg
//
LOCUS       DASRSC010000281         9530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2076160,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000281 DASRSC010000000
VERSION     DASRSC010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9530
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076160"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VFE39_13920"
     CDS             complement(<1..331)
                     /locus_tag="VFE39_13920"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700675.1"
                     /note="catalyzes the transamination of the branched-chain
                     amino acids to their respective alpha-keto acids; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid aminotransferase"
                     /protein_id="HET6259740.1"
                     /translation="MTPQFTRTINPAPASKARRDDVLADPGFGRYFSDHMVTIRWTVD
                     RGWHEPAVLPYGPLMLNPASMVLHYGQEIFEGLKAYRQPDGSIASFRPDANAARFRGS
                     AMRMAMPE"
     gene            complement(400..1497)
                     /gene="gcvT"
                     /locus_tag="VFE39_13925"
     CDS             complement(400..1497)
                     /gene="gcvT"
                     /locus_tag="VFE39_13925"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HET6259741.1"
                     /translation="MDDLLTSPLHDRHTALEASLGAFGGWSMPLSYPDGTVAEHTAVR
                     EAVGVFDVSHLGKLAITGPGAADFVNRCFTADLGKIGPGHAQYTLCCTEDGGVVDDII
                     VYLVGPDEVLAVPNAANAARVASLLRDAAPAGIEIVDRHRELAVIAVQGPRSPELFAS
                     MFAGFETVAGLDYMAFADAGQVRVCRTGYTGERGYELLVPAADAAGVWDALLAGAHAV
                     GGRPCGLGARDTLRTEMGYPLHGQDLSVDISPVQAGSGWAVGWSKPEFWGRDALLAEK
                     EAGPARRLRGLRATRRGVPRAGMAVLRNGEQVGTTTSGTFSPTLKTGIALALIDTASG
                     VALDDHVTVDVRGRTLECAVVKPPFVPSHVR"
     gene            complement(1509..1925)
                     /locus_tag="VFE39_13930"
     CDS             complement(1509..1925)
                     /locus_tag="VFE39_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6259742.1"
                     /translation="MEFVQSVTIQAAGDRVWAVLSDVERWPEWTASMNAVRRLEAGPL
                     VTGSRAEVRQPRLPLARWEVTELDPARGFTWVATGPGIRTVGEHIMEAGRSCTVVRLR
                     LLQQGPMGGVLGLLTRGLTRRYLAMEAEGLKRRCEQ"
     gene            1978..2529
                     /locus_tag="VFE39_13935"
     CDS             1978..2529
                     /locus_tag="VFE39_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004561145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6259743.1"
                     /translation="MSAKDELLAAALEHVTAHGVGALTLRRLAAELGTSHRMLIYHFG
                     SWEGLLVAIVQAVEQRQRDRLRALLAEPDLPPGEQARRFWAQLVDPAQWPSERLFFEL
                     YGQALQGRPHAVPLLDGVVDDWLDPLTELTVRSGVPRAEARAHARLRLAVARGLLLDL
                     LATGDVEGVRAAAEVHAAAYRPA"
     gene            complement(2545..3807)
                     /locus_tag="VFE39_13940"
     CDS             complement(2545..3807)
                     /locus_tag="VFE39_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019044553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HET6259744.1"
                     /translation="MGTVSWSVQRRLGTTDAVVLGLAAMLGTGVFAVWAPAAAAAGPW
                     LLLAVVRAGVVAACNAASTADLAVAHPESGGGYVYGRERLSPGAGRLAGVAFLVGKTA
                     SAAAAAGVFGSYVLPSQPLPAAILVIVAATVLNTAGVRWTARGAYALVGGTLAVLLVV
                     VVVGLLGPQVDTMASAATPEVERVAMPGGVLGVLTAAGLVFFAFAGYARIATLGEEVR
                     DPDRTLRRAIAVALGIALVTYVLVAVALLVGLGAERLATEPAPLVALVDTGQASALGV
                     LVRLGAAVAAGSALLSVLVGVSRTALAMARRRELPGALARVCSRGTPRWADLAGGVVA
                     IVLAMLAGPVAAIALSACSVLVYYAVINLAALRLPRANRRWPAWTSVLGLLMCVGLAV
                     LLPVQQVVITAAALAVGWVICTAFALRP"
     gene            3968..5470
                     /locus_tag="VFE39_13945"
     CDS             3968..5470
                     /locus_tag="VFE39_13945"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HET6259745.1"
                     /translation="MSTDLHVASGSPATAETDAVVVGLLPATGDNSGATLAPGADEVA
                     DAFGTGDSGLVALLTVAGATGKADEVVKLPTRGAITAPLLVAVGLGEPEADGSPSAEA
                     VRRASGAAARALAGTASAVSTLGRINLAAAAEGTLLGAYTFSAYRSNGAGRAPVARVG
                     LVGGGDAEETVRTATAVATAVRTARDLVNTPPNDLFPESFAARARELAEAAGVEVEVL
                     DDAALAEGGYGGVLGVGQGSSRGPRLVRLRYAGGPAPRARVALIGKGITFDTGGISIK
                     PAANMDHMTSDMGGAAAVIVTTLLAAQLELPVAVTATVPMAENMPSATAYRPGDVLRM
                     YGGKTVEVLNTDAEGRLILADAIARAAEDEPDYLVETSTLTGAQQVALGLRTAGVMGS
                     DEFRDRVAEHARITGEDGWAMPLPAHMRSELDSRVADIANVTGSRWGGMLVAGTFLRE
                     FVPDGLPWAHIDIAGPSFHTGGPYGYTTKGGTGVPVRTLHALLTDIAKNG"
     gene            complement(5565..5912)
                     /locus_tag="VFE39_13950"
     CDS             complement(5565..5912)
                     /locus_tag="VFE39_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HET6259746.1"
                     /translation="MKLLGMLGMGRRGTLRTADRDDQAHLRAWAEAHEGVEGFVEPRT
                     TVTAMTILLVDRDGEWTRRRIPSADAARRLARSLKMPVYDVQLVGYPQRMRDHDARQR
                     ILRDRQRRENLGG"
     gene            6147..7529
                     /gene="lpdA"
                     /locus_tag="VFE39_13955"
     CDS             6147..7529
                     /gene="lpdA"
                     /locus_tag="VFE39_13955"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET6259747.1"
                     /translation="MPEHNADLVILGGGSGGYACALRAAELGMSVVLVERDKLGGTCL
                     HYGCIPTKALLHAAEVADNAREGDKIGVKSSLAGIDMAGVNSYKDGVIAKLYKGLQGL
                     VKSRKITLVDGTGRFEGPNAVVVGEDRYVGKNVVLATGSYSKTLPGLDIGGRIITSSE
                     AIHLDEVPDRIVILGGGVIGVEFASVFKSFGSDVTIVEALPRLVPNEDEFASKLLERA
                     FRRRKITFKTGVRFTGAKQSDDAVTVSLESGEEIEADLLLVAVGRGPNTANAGFEEAG
                     VRMERGFVLADERLRTNLPNVFALGDIVPGLQLAHRGFAQGIFLAEDIAGLNPPVIDE
                     AGIPRVTYCEPEVASVGLTEAQAKEKHGEIQTLTYDLAGNGKSQILQTQGAIKLIKTG
                     AAGTPGPVVGIHMVGARVGELIGEAQLVYNWDAQAEDVAALVHAHPTQNEAFGEAHLA
                     LAGKPLHSHS"
     gene            7598..9487
                     /gene="sucB"
                     /locus_tag="VFE39_13960"
     CDS             7598..9487
                     /gene="sucB"
                     /locus_tag="VFE39_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HET6259748.1"
                     /translation="MAFSVQMPALGESVTEGTVTRWLKQEGDRVEVDEPLLEVSTDKV
                     DTEIPSPAAGVLQRIVAGEDETVEVGGELAVIGDGSESGSDSGGDVEGGGQGGNTAAQ
                     SAVEAPAEEEADAGGAPAPPNQAESARSDQPTSAPRAATESTGSGTAITMPELGESVT
                     EGTVTRWLKQVGDEIAVDEPLLEVSTDKVDTEIPSPIAGTLLEITAGEDETVEVGGQL
                     AVIGDATEAPSAGTPSGSSPADSAPPDDAPAPERRPEPPSEQDQAAEQPSSNGKVAQA
                     VEQADEQAERDDLPSEQPERPGDDDGGAGRAPYVTPLVRKLAAEHDVDLSTISGSGVG
                     GRIRKQDVLAAAESRRPAPEPEPEAAPEAPAAAAPASAPSAAPARQVPKPAADGPQPG
                     TTVKMPRLRQVIAQRMTESLQVSAQLTTVQDVDVTRIAKLRARAKAEFERREGVKLTF
                     LPFFAKAAVEALKAFPQLNASINVESREVTYHGAVHLAVAVDTPRGLLVPVIKNAEDL
                     NIAGLARRIADVAARTRANKIGPDELSGGTFTLTNIGSAGALFDTPIINQPQVAILGT
                     GKITKQPTVVTGPDGEDSIAIRSVCFLPLTYDHRLVDGADAGRFVSAIKARLEEGAFE
                     ADLGL"
BASE COUNT         1455 a         3530 c         3354 g         1191 t
ORIGIN      
        1 gctcgggcat cgccatccgc atcgcggagc cgcggaagcg ggcggcattg gcatcgggcc
       61 ggaacgaggc gatcgagccg tccggctgcc ggtaggcctt gagcccctcg aagatctcct
      121 gcccgtagtg cagcaccatc gaggccgggt tcagcatcag cggcccgtag ggcagcacgg
      181 ccggctcgtg ccaaccccgg tccaccgtcc agcggatcgt gaccatgtgg tcgctgaagt
      241 accggccgaa ccccgggtcg gcgagtacgt cgtcacgccg ggccttcgat gccggtgcgg
      301 ggttgatggt ccgggtgaac tgcggggtca tggtttcaca ttatctggtc gagcaagccc
      361 cgattgtcgg acgtccgagc tgtgctgtcg gccgtacggc taccgcacgt gggaggggac
      421 gaacggtggc ttgaccacgg cgcactccag agtccgaccg cggacgtcga cggtgacgtg
      481 gtcgtccagc gcgacgccgg acgcggtgtc gatgagcgcc agtgcgatgc cggtcttcag
      541 cgtcggggag aacgttccgg acgtggtggt gcccacctgc tcgccgttgc gcagcaccgc
      601 catgcctgca cgcgggacgc cgcggcgggt ggcccgcagc ccgcgcaggc gccgcgcagg
      661 cccggcctcc ttctcggcga gaagcgcgtc gcggccccag aactcgggct tcgaccaacc
      721 gaccgcccaa ccgctgcccg cctggacggg cgagatgtca acggacaggt cctggccgtg
      781 cagggggtag cccatctcgg tgcgcagggt gtcgcgcgcc cccagcccgc acggccgccc
      841 gcccaccgcg tgcgccccgg ccaggagcgc gtcccacacc ccggcggcgt cggcggcggg
      901 cacgagcagt tcatagccgc gttcgccggt gtagccggtg cggcacaccc gcacctgccc
      961 ggcgtcggcg aaggccatgt agtccagccc ggccacggtc tcgaagccgg cgaacatgct
     1021 cgcgaacagt tcgggcgagc gtgggccctg cacggcgatc accgcgagct cgcggtgccg
     1081 gtcgacgatc tcgatgccgg ccggggccgc atcgcgcagg agcgaggcga cacgagcggc
     1141 attggcggcg ttggggacgg cgagtacctc gtccggtccg acgaggtaca cgatgatgtc
     1201 gtcgacgacc ccgccgtcct cggtgcagca cagcgtgtac tgcgcgtgcc ccggtccgat
     1261 cttccccaga tctgcggtga agcagcggtt gacgaaatcc gcggcgcccg gcccggtgat
     1321 cgccagcttg ccgagatggc tcacgtcgaa gacgccgacg gcctcgcgca cggccgtgtg
     1381 ctcggccacc gtgccgtcgg ggtaggacag cggcatcgac cagccgccga acgctccgag
     1441 gctggcttcg agcgctgtgt gccggtcgtg taacgggctg gtcaggaggt cgtccacgga
     1501 cggggacgct actgctcgca cctgcgtttc aggccctcgg cctccatcgc gaggtagcga
     1561 cgggtgagcc cgcgggtgag cagcccgagc accccgccca tcggtccctg ctggagcagc
     1621 cgcagccgta ccacggtgca gcttcggccg gcctccatga tgtgctcacc gaccgtccgg
     1681 atcccgggcc cggtcgcgac ccaggtgaag cccctggccg gatccaactc ggtgacctcc
     1741 caccgggcga gcggcaggcg gggctggcgc acctcggcgc ggctgcccgt gacgagcgga
     1801 cccgcctcca gccggcgcac cgcgttcatg gaggcggtcc actccggcca gcgctcgacg
     1861 tcggagagca cggcccagac ccgatcacct gctgcctgga tcgtcaccga ctgcacgaac
     1921 tccacggcac ccctcccggt gatgtagtaa ccgggacgga aacgtaccat atggtacatg
     1981 agcgcgaagg acgagctgct cgccgcagcg ctcgagcacg tgaccgccca cggtgtcggc
     2041 gctctcaccc tgcgccggct cgccgccgag ctcggcacca gccaccggat gttgatctat
     2101 cacttcggct cgtgggaggg cctgctcgtc gcgatcgtgc aggccgtcga gcagcgccag
     2161 cgcgaccggc tgcgggcgct gctggccgaa cccgacctcc cacccggcga gcaggcgcgc
     2221 cggttctggg cacagctggt ggacccggcg cagtggccgt cggagcggct gttcttcgag
     2281 ctctacggac aggcgctgca gggcaggccg cacgccgtcc cgttgctcga cggcgtggtg
     2341 gacgactggc tcgacccact caccgagctc accgtgcgca gcggagtccc ccgcgccgag
     2401 gccagggcac acgcccggct gcggctcgcc gtcgcccgcg gcctgctgct ggacctgctg
     2461 gcgaccggtg acgtcgaggg cgtccgcgcc gccgccgagg tgcacgcggc ggcgtacagg
     2521 cctgcctgag caccgttgcg gggatcaggg gcggagggcg aacgcggtgc agatcaccca
     2581 gccgacggcg agggcagcgg cggtgatcac cacctgttgc acgggcagca gcaccgcgag
     2641 gccgacgcac atcagcaggc cgagcactga tgtccaggcc ggccagcgcc ggtttgcccg
     2701 gggcagccgc agggcagcca ggttgatcac ggcgtagtag acgagcaccg agcaggccga
     2761 cagggcgatg gccgccaccg gcccagccag catggcgagc acgatcgcga cgacgccgcc
     2821 cgcgaggtcg gcccagcgcg gcgtgccccg ggagcagacc ctggcgagtg cgccgggcag
     2881 ctcacggcgc cgtgccatcg ccagtgccgt gcggctcacc ccgaccagca ccgacagcag
     2941 ggctgaaccc gccgccacgg ctgcgccgag ccgcacgagc acgccgagcg cgctcgcctg
     3001 cccggtatcg acgagcgcaa ccagcggcgc gggctccgtg gccagccgtt cggcgccgag
     3061 gcctaccagc agcgcgaccg ccacgagcac gtaggtgacc agcgcgatgc ccagcgcgac
     3121 ggcgatggcc cggcgcagcg tccggtcggg gtcgcgtacc tcctcgccca acgtggcgat
     3181 gcgcgcgtac ccggcgaagg cgaagaacac cagcccggcg gcggtgagca ccccgagcac
     3241 cccgcccggc atcgccaccc gctccacctc cggggtggcg gcggaggcca tggtgtcgac
     3301 ctgcgggccg agcaacccga cgacgaccac gaccagcagg accgccagcg tgccgccgac
     3361 cagcgcatac gccccgcgtg ccgtccagcg cacgcccgcg gtgttcagca ccgttgcggc
     3421 gacgatcacc aggatcgccg ccggcagggg ttgcgacggg agcacgtagc tgccgaacac
     3481 acccgccgcc gcggcggccg acgccgtctt gcccaccagg aacgcgacgc ccgcgagccg
     3541 cccggccccc ggcgagagcc gctcccgccc gtagacataa ccgccgccgc tctccgggtg
     3601 ggcgacggcg agatcggcgg tggacgcggc gttgcacgcc gcgacgaccc cggcccgcac
     3661 gacggcgagc agcagccagg gtccggctgc cgctgcggcg ggagcccaga cggcgaatac
     3721 tccggtaccg agcatggccg ccagcccgag cacgaccgcg tcggtcgtgc cgagtcggcg
     3781 ctgcacgctc caggacacgg tgcccaccct tgcacccggg cctacctgat ccggctgcac
     3841 actggtgtca ttaccatctc gaagcgatct cgctcacggg cgagccggac cctgccgggc
     3901 cggcggaacc ggaacgcccg ccaccgaata cccatctctt actcatcgtt cgtcgagagg
     3961 acggcccgtg tcgaccgatc tgcacgtggc aagcggctcc cccgccaccg ccgaaaccga
     4021 cgccgtggtc gtcgggctgc tgccggccac gggtgacaac tccggggcga ccctcgctcc
     4081 cggcgccgac gaggtggccg acgcgttcgg caccggtgac tcggggctcg tcgccctgct
     4141 cacggtggcg ggggccaccg ggaaggccga cgaggtggtc aagctgccca cgcgcggagc
     4201 gatcacggcg ccactgctcg tcgcggtcgg cctcggcgag ccggaggccg acgggtcgcc
     4261 gagcgcagag gccgtccgcc gggcctccgg cgccgccgct cgcgctctcg ccggcaccgc
     4321 gagcgcggtc agcaccttgg gccgcatcaa cctggccgca gccgccgagg gcaccctgct
     4381 cggtgcctac acgttcagcg cctaccgcag caacggcgca gggcgcgcgc ccgtcgctcg
     4441 cgtcggcctg gtcggcggtg gcgacgccga ggagaccgtg cgcaccgcca ccgcggtcgc
     4501 gacggccgtt cgcacggcgc gcgacctcgt caacaccccg cccaacgacc tgttccccga
     4561 gtcgttcgcg gcccgggcgc gcgagctggc cgaggccgcc ggtgtcgagg tcgaggtgct
     4621 ggacgacgcc gcgctcgccg agggcgggta cggcggcgtg ctcggcgtcg gacagggctc
     4681 gtcgcggggg ccccgactgg tccggctgcg ttacgcgggc ggcccggcac cccgggccag
     4741 ggtggcgctc atcggcaagg gcatcacgtt cgacaccggt gggatctcca tcaagcccgc
     4801 cgcgaacatg gaccacatga cgtccgacat gggcggagcg gccgcggtga tcgtcaccac
     4861 cctgctggcc gcgcagctgg agcttccggt ggcggtcacc gcgacggtgc cgatggccga
     4921 gaacatgccc agcgccacgg cgtaccggcc cggcgatgtg ctgcggatgt acggcggcaa
     4981 gacggtcgag gtgctcaaca ccgatgccga gggacggctg atcctggccg acgcgatcgc
     5041 cagggccgcc gaggacgagc ccgactacct ggtcgagacg tccacgctga cgggcgccca
     5101 gcaggtggcg ctcggcctgc gcaccgcggg tgtgatgggc agcgacgagt tccgggaccg
     5161 ggtggccgag cacgcccgga tcaccggcga ggacggctgg gccatgccgc tacccgccca
     5221 catgcgcagc gagctggact cccgcgtcgc cgacatcgcc aatgtcacag gcagccgctg
     5281 gggcgggatg ctcgtagccg ggacgttcct gcgtgagttc gtgccggacg gcctgccgtg
     5341 ggcacacatc gacatcgcag gcccgagctt ccacaccggc ggcccgtacg gctacacgac
     5401 gaagggcggc accggcgtgc cggtccgcac cttgcacgca ctactgaccg acatagcgaa
     5461 gaacggctga ggctaggccg tcctcgcaga ggcactggtc gcgtcgcaga ggccgtgggg
     5521 cctgcgacga ggacagctgg tctgcgagga cgccccgcct gcggctatcc gcccaggttc
     5581 tcgcggcgct ggcggtcgcg gaggatccgt tgcctcgcgt cgtggtcacg catccgctgc
     5641 ggatagccca cgagctgcac gtcgtacacc ggcatcttca acgagcgcgc cagccggcgt
     5701 gccgcgtcgg ccgacgggat ccgccgccgc gtccactccc cgtcgcggtc gaccagcaga
     5761 atcgtcatcg cggtgacggt ggtgcggggc tccacgaacc cctccacacc ctcgtgcgcc
     5821 tccgcccacg cacgcaggtg tgcctgatcg tcacgatccg ccgtccgaag cgtgccccgg
     5881 cggcccatgc ccagcattcc gagtagcttc accgcactcc ctcgctgcgc tcggccggcg
     5941 cctcgcgcgg cccccgtatc catggtttgc tcgcaggttc gctcatcgcg acccacctcc
     6001 ccactggcac caacgctcga ggtcgatggt tttctcccgc gtgcaccagc cgccacgccc
     6061 gtccaccacg gatgacaaga tgtgaacagc ggcgtcaccc gccgctgaca cacgcactgc
     6121 cggcaattcg gtgagggagt caagacgtgc ccgagcacaa cgcggacctg gtgatcctgg
     6181 gcggtggatc gggcggctac gcctgcgcgc tgcgggcggc tgagctgggc atgtcggtcg
     6241 tgctggtcga gcgcgacaag ctcggcggca cctgcctgca ctacggctgc attcccacca
     6301 aggcgctgct gcacgccgcc gaggtggccg acaatgcccg cgaaggcgac aagatcggcg
     6361 tcaagagctc gctggccggc atcgacatgg ccggcgtgaa ctcctacaag gacggcgtca
     6421 tcgccaagct ctacaaggga ctgcagggac tggtgaagtc tcgcaagatc actctggtcg
     6481 acggcaccgg gcgcttcgag ggccccaacg cggtcgtcgt cggcgaggac cggtacgtcg
     6541 gcaagaacgt cgtgctggcc accggcagct actccaagac ccttcccggc ctcgacatcg
     6601 gcggccggat catcaccagc agcgaggcca tccacctgga cgaggtcccg gaccggatcg
     6661 tgatcctcgg cggtggagtc atcggagtcg agttcgcgtc ggtgttcaag agcttcggct
     6721 cggacgtcac catcgtcgag gcgctgcctc ggctggtccc caacgaggac gagttcgcct
     6781 ccaagctgct ggagcgggcg ttccgccgcc gcaagatcac cttcaagacg ggcgtcaggt
     6841 tcaccggggc caagcagagc gatgacgccg tcaccgtctc gctcgagtcc ggcgaggaga
     6901 tcgaggccga cctgctgctg gtcgctgtcg gtcgtggccc gaacaccgcc aacgcggggt
     6961 tcgaggaggc cggcgtgcgg atggagcgcg ggttcgtgct cgccgacgag cggctgcgca
     7021 ccaacctgcc gaacgtcttc gcgctgggcg acatcgtccc cggcctccag ctcgcccacc
     7081 gcgggttcgc ccaggggatc ttcctcgccg aggacatcgc cggcctcaac ccaccggtga
     7141 tcgacgaagc cggcatcccc cgcgtcacct actgcgagcc cgaggtcgcg tccgtggggc
     7201 tcacggaggc gcaggccaag gagaagcacg gcgagatcca gacgctcacc tacgacctcg
     7261 cgggcaacgg caagagccag atcctgcaga cgcagggcgc gatcaaactg atcaagaccg
     7321 gggccgcggg cacgccgggc ccggtcgtcg gcatccacat ggtcggcgcc cgcgtcggcg
     7381 aactcatcgg ggaggcccag ctggtctaca actgggacgc ccaggccgag gacgtcgccg
     7441 cgctggtgca cgcccacccc acccagaacg aagcgttcgg cgaggcccac ctcgcgctgg
     7501 ccggcaagcc gctgcacagc cactcgtgac caggcgcagc cgaccgctgc tccggccacc
     7561 cgaactcgaa ccgcaggaag aggagctccg acccgccatg gccttctccg tccaaatgcc
     7621 cgccctcggt gagagcgtca ccgagggcac cgtcacccgg tggctcaagc aggagggcga
     7681 ccgggtcgag gtcgacgagc cactgctcga ggtctccacc gacaaggtcg acaccgagat
     7741 cccgtctccc gctgcaggcg tgctgcagcg gatcgtcgcg ggtgaggacg agacggtcga
     7801 ggtcggcggc gagctcgccg tgatcggcga cggctcggag tccggttccg actccggcgg
     7861 cgacgtcgag ggcggcggcc agggcggcaa cactgcggcg cagtcggccg tcgaggcccc
     7921 ggccgaggaa gaggccgacg caggcggggc gcctgcgccg ccgaaccagg cggagtccgc
     7981 ccggtccgac caacccacct cggcgccccg ggcggccacc gagtccacgg gctccggcac
     8041 cgcgatcacg atgccggagc tcggtgagag cgtcaccgag ggcaccgtca cacgctggct
     8101 caagcaggtg ggtgacgaga tcgccgtcga cgagccgctg ctggaggtgt ccaccgacaa
     8161 ggtcgacacc gagatcccct cccccatcgc cggcacactg ctcgagatca ccgcgggcga
     8221 ggacgagacg gtcgaggtcg gcggtcagct cgccgtgatc ggtgacgcca ccgaggcccc
     8281 gtccgctggt acgccctccg gcagttcccc ggccgacagc gccccgcccg acgatgcccc
     8341 ggctcccgag cgcaggccgg agccgccgag tgagcaggac caggccgccg agcagccctc
     8401 gagcaacggc aaggtcgcgc aggccgtgga gcaggccgac gagcaggccg agcgcgacga
     8461 ccttccctcc gagcagcccg aacggcccgg ggacgacgac ggcggcgcag gccgggcccc
     8521 gtacgtcacc ccgctggtcc gcaagctggc cgcggagcac gacgtcgacc tctcgacgat
     8581 ctctggctcc ggcgtcggcg gccgaatccg caaacaggac gtcctcgccg ccgccgagtc
     8641 ccgcaggccc gccccggagc ccgagcccga ggcggccccg gaggctccgg ctgcggcagc
     8701 cccggcgagc gcaccgtcgg ccgccccggc gcggcaggtg ccgaagcccg cggcagacgg
     8761 cccgcagccg ggcaccacgg tgaagatgcc ccggctgcgg caggtgatcg cgcagcggat
     8821 gaccgagtcg ttgcaggtgt cggcccagct cacgaccgtg caggacgtcg acgtcacgcg
     8881 catcgccaag ctgcgtgccc gcgcgaaggc ggagttcgag cggcgtgagg gtgtcaagct
     8941 gacgttcctg ccgttcttcg ccaaggccgc cgtcgaggcg ttgaaggcct tcccccagct
     9001 caacgcgtcg atcaacgtgg agagcaggga ggtgacctac cacggggccg tacacctggc
     9061 ggtggcggtg gacacccccc gcgggctgct ggtccctgtg atcaagaacg ccgaggacct
     9121 caacatcgcg gggctggccc ggcggatcgc cgacgtggct gcgcgcaccc gggccaacaa
     9181 gatcggcccc gacgagctct ccggcggcac gttcacgctc accaacatcg gcagcgccgg
     9241 tgcgctcttc gacacaccga tcatcaacca gccgcaggtc gccatcctgg gcacgggcaa
     9301 gatcacgaag cagcccacgg tggtcaccgg gccggacggc gaggactcca tcgccatccg
     9361 ctccgtctgt ttcctgccgc tcacctacga ccaccgcctg gtcgacgggg ccgacgccgg
     9421 ccggttcgtc agtgcgatca aggcccgcct ggaggagggc gcgttcgagg ccgacctcgg
     9481 gctctgacgg ctcaggtcgc cggggatacc ggtcgggtgg cccgagtcgc
//
LOCUS       DASRSC010000282        18557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5352826,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000282 DASRSC010000000
VERSION     DASRSC010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 18557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18557
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5352826"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..729
                     /locus_tag="VFE39_13965"
     CDS             40..729
                     /locus_tag="VFE39_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6259749.1"
                     /translation="MLVAGFPAGSFQTNCYVVAADAGEACVVVDPGQDAVEPLEALLA
                     EHRLTPVAVLLTHGHFDHTFSVAPVCDGHDVPAWIHPEDRSMLADPMKGISAEAAAFF
                     GGRMELREPREVRELTDGAALDIAGFTLRVDHTPGHTRGSVVFTTPTEEGVEVILAGD
                     TLFAGSIGRTDLPGGDHAQMLASLRDKLLTRDDSAVVLPGHGPTTTVGRERASNPFLQ
                     GLELPTTGRGL"
     gene            780..2039
                     /gene="hisS"
                     /locus_tag="VFE39_13970"
     CDS             780..2039
                     /gene="hisS"
                     /locus_tag="VFE39_13970"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET6259750.1"
                     /translation="MSAFAAPKGIPEYVPPDSAGFEHVHNTLVSAADRAGYGLIELPV
                     FEETALYTRGVGESTDVVSKEMYTFADRGERSVTLRPEGTAGVVRSVIEHGLDRAGLP
                     VKLRYAGPFFRYERPQAGRYRQLQQVGIEAIGVDDPALDAEVIAVADEGFRALGLTGY
                     RLEITSLGDTESRPPYRAMLQEFLAGLPLDEATRMRAEINPLRVLDDKRTEVRALLSD
                     APLLVDHLSAASAEHHAAVKQHLDDLGVAYVENPRMVRGLDYYTKTTFEFVHDGLGAQ
                     SGIGGGGRYDGLMATLGGQPLSGVGFGIGVDRTLLACRAEGVAPWSEARCEVFGVPLG
                     DAAKRRLVVLAAQLRRVGVRVDLAYGGRGLKGAMKAADRSGARFALVLGERDLEAGTV
                     GLKDLASGEQQDVALADAADEVLTRLR"
     gene            2076..2633
                     /locus_tag="VFE39_13975"
     CDS             2076..2633
                     /locus_tag="VFE39_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6259751.1"
                     /translation="MATEDRERWEARHAAVSSAGAPDIPMPPDALRGRERLLPDGGRA
                     LDVACGRGTVAGWLAERGFVVDAVDISEAAVAAGAALVVDRGVAERVRWWRYDLDAGL
                     PGGCAGPYDVVVCQRFRDPALYPVLAQRLAPGGLLVLTVQSVVGDEPGRFRASPGELL
                     AAFAELTTLHHTESDGQASLLARRR"
     gene            complement(2740..4329)
                     /locus_tag="VFE39_13980"
     CDS             complement(2740..4329)
                     /locus_tag="VFE39_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005159041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HET6259752.1"
                     /translation="MPTDEPGLPPASEQPGTAGRDTARHRFGRSAGAATTPPPAAFPL
                     PPRPGSGRHGHPDRMGQLDDADEAAAEAPTPPTGWTPVDTRTVRTSASRTAAARGPRT
                     AGRSPGQGDRDEEQADRVVRSGPQAAVATSTASAREPAHGHGHGHSPAPPAGGRVRLL
                     IATLLVPCALATLAGVVLLWPAGGPPTTAQPVTQQPVHAEVVSASASDCSAGSGDGSC
                     VALVVRMADGPMPGRDLVQVVPVEPGSPTFAVGDRVVLGWSGANPNDPGSYQVVDFQR
                     GAPLGWLAAIFAGAVLLLGRWRGLAALAALVLSFAVLLLFVLPSILAGHDPLAVAVVG
                     SCLIMFVVLYLTHGLSARTSTAVLGTLLSLALIGGLGAAFSVAARLTGLDDQTSNLIA
                     TLGTGVDARGLLLAGVLIGALGVLDDVTVTQTSAVWELRRANPELGARALFGAAMRIG
                     RDHVASAVNTLVLAYAGASLPLLLLFTLSGRALGDVVTTQDVATEVVRTLVGSIGLVA
                     SVPITTALAAMVASRESGSTV"
     gene            complement(4404..4940)
                     /locus_tag="VFE39_13985"
     CDS             complement(4404..4940)
                     /locus_tag="VFE39_13985"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HET6259753.1"
                     /translation="MTVTLVVMGVSGVGKSSVAAELVRRTGWVFVEGDDLHPESNRAK
                     MAAGIPLDDDDRWPWLRRVAAWIGEQEAADRSAVVTCSALKRSYRELLQDGHPSVRFV
                     HLLAPPELIAARVTARRNHYMPPSLLDSQLAALEPLGPDEPGIGVDTTGDAAAVAERA
                     LQQLGGDTSEEAADDRSR"
     gene            5010..7085
                     /locus_tag="VFE39_13990"
     CDS             5010..7085
                     /locus_tag="VFE39_13990"
                     /inference="COORDINATES: protein motif:HMM:NF038135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intein-containing Rv2578c family radical SAM
                     protein"
                     /protein_id="HET6259754.1"
                     /translation="MRWSGQQVLDDGVGAEAALPGLRGLLRSVRTPEFAGAVFHEVEA
                     KSVLNRVPGSSPMPFRWTVNPYRGCSHACVYCLAGPTRVLLADGRTRPIAELRVGDAV
                     MGTELVDGQRRYVRTTVRAHWSTTKPAHRLRLAGGSELVASGEHRFLTDQGWRHVSGG
                     WCRSGRRPRLRTGSALLGPGPAAMVGWQRRPHTAAYRQGYLCGLVRGDGLGADGERCF
                     PSEHLELEALGRAHHFLAEAAQHVVELPSAVPVPVPVGAGEPGRIPAPTPRGGIAPIT
                     DVVRWPEQPGDDWCAGFLAGTVDARGSVVRGELRVRHAGEEIAGRAARALHRLGFRFA
                     VEADHGSGPGSSAGRTVRLLGGSAALLRLLQVVDPAVSRHRDVGGAAVTGGTALVVES
                     VQPLGVEMPMYDITTGTGDFVAEGVVSHNCFARNTHTYLDLDAGADFDSQIVVKVNVA
                     RVLERELRSAKWAREPVAMGTNTDPYQRAEGRYRLMPGVIGALARSGTPFSLLTKGTV
                     LTRDLPQLAAAAADVPVGLGVSIALVDRSLQHRLEPGTPSPAARLDLVRRITDAGLSC
                     GVMVAPVLPLLSDSVEALDTLLAQVKQAGANGASVLALHLRPGTREWFLAWLAREYPK
                     LVAPYARLYRRGAYVDPEYRRALGERVAPLLRRHGLSGAATTVRGQGWPAARAPTVET
                     PSGEQLSLL"
     gene            7321..8328
                     /locus_tag="VFE39_13995"
     CDS             7321..8328
                     /locus_tag="VFE39_13995"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HET6259755.1"
                     /translation="MSRRTLEAAGAIVAVAGLSFVGLSAEADAAAQPSPAALSGGTGM
                     SMPMDSIGAYDPQNHSDATFFASVLKGANEVPVTSGPKVGDKNGSATALMRIQGDQVS
                     YAFTWTGIGTPTMGHIHEGPTANGDVQIPFFGTKLPAGRNSVIGTVKVTNKDLLANLK
                     THPEQFYFNLHTAEFPGGAVRGQVHALPEKLNLQAAMTADQVHSVVRGAQIYACTRHN
                     DGSFTFTQNNVDATLGGGIRHTFLKAGPAGPPQWVAPDGSGVTGKALTMIPNGGKNIP
                     ELVLAATPEGRPGGVLSSTQIVMRLNTVGGVAPAGTCNPAAQPKAAVPYTADYLFVTS
                     T"
     gene            8495..10303
                     /gene="aspS"
                     /locus_tag="VFE39_14000"
     CDS             8495..10303
                     /gene="aspS"
                     /locus_tag="VFE39_14000"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018681976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET6259756.1"
                     /translation="MMRTHPAGTLRAEHAGQSVTLAGWVARRRDHGGVIFIDLRDASG
                     SAQVVFREGEMAERAHRLRAEFCVVVTGEVVRRPAGNENPELATGAIEVTATELTVLS
                     ESAPLPFPLDEHSEIGEEIRLKHRYLDLRRSGPAAALRLRSEANRIARTVLHDREFVE
                     VETPTLTRSTPEGARDFLVPSRLRPGSWYALPQSPQLFKQLLMVAGTERYYQIARCYR
                     DEDFRADRQPEFTQLDIEMSFVEQDDVIELGETIVSALWSELAGYEIPRPIPRLTYAD
                     AMARYGSDKPDLRFGVELTDLTEYFADTPFRVFQAPYVGAVVMPGGASQPRKQLDAWQ
                     EWAKQRGARGLAYVLVDDDGSVSERGPVVKNLSEAEREGLPKAVGAEPGDAVFFAAGR
                     PADARELLGAARGEIARRCGLVDENAWSFVWVVDAPMFERVRDDKGDEVGWTAVHHPF
                     TSPNEEWMDRFEEAPDQALAYAYDIVCNGNEIGGGSIRIHRSDIQQRVFALLGMNEAE
                     QREKFGFLLDAFQYGPPPHGGIAIGWDRTCMLLAHSDSIRDVIAFPKSGGGFDPLTGA
                     PAPITPEQRKEAGVDAQPPAAPAAASPAADEPPTKL"
     gene            10344..11285
                     /locus_tag="VFE39_14005"
     CDS             10344..11285
                     /locus_tag="VFE39_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HET6259757.1"
                     /translation="MLHMRVISPVGQTDAVRDLLLGEPGATHVTVLPGIALQPPGDVV
                     EADVARESVDPLLGALSELGVDRAGGVTLEVIDTSLSDAADAAEEAAPGDPSDAVIWD
                     ELVARTGEESRLSVSFQAFLTIACLLAAIGAITNSPVTVVGAMVLGPEFGPLAAIAVG
                     AVLRRGDLVRRGGLALAVGFPLAMVITALAAVGFDATGLLSTATLDNLDQVDFIYKVG
                     PFSFIVALLAGAAGMLALTSAKSASLVGVFISVTTVPAAAFASTALVEGRYAQAAGSA
                     LQLLVNLVGIVVAATAVLLVSHRTAVRERGMRRLSAG"
     gene            11297..12490
                     /locus_tag="VFE39_14010"
     CDS             11297..12490
                     /locus_tag="VFE39_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259758.1"
                     /translation="MVRDLGSFPRPVLAALAAAERLLSRRSGAGIVLADPEDLGGSDR
                     SVVARARVARNPFSLPRTLVVKHYLGRPDPDRPDPFHYEVASCQLFTALPAEARPSPV
                     LIAHDPQSRLLVLEDLGRSSTLADKLFGPDPAAAQRCLLSWARALGRMQAATAGREND
                     FGALLRRLGERAWRDPMAEDARVALADVPGLLEQDLGVTVSPAASLEAQETARLLGGT
                     RYRAFSPSDTCPDNNLVTSRGVRFVDFEWGCFRDVALDAAYFRVPFPGCEASFAMPPG
                     MAETMLEAWRNEIAAVWPDLDEPDRLDRRLLHAQLLWVWLCTWWLLPRIRVRDMPVGL
                     DATRSPRISTALGHYWREMARTAATGGLPATAELGTMVADALAKRFPDAPGSLPVFPA
                     FRSVA"
     gene            12611..13453
                     /locus_tag="VFE39_14015"
     CDS             12611..13453
                     /locus_tag="VFE39_14015"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6259759.1"
                     /translation="MTVQPLLAPLATAPITPGMSTAPAGTPQVGTLVTLGDSTSVGLG
                     DPVPGGGWRGFPVLLRDALGAPTLVNLGRTGARMACVRDEQLPVALLAAPDVAVLFVG
                     MNDTLRSDFDPETLHAHCSAVVGALRAGGAHVLMLRYHDHTQVFRLPAPLRRALYRRI
                     VALNAVTDAVAAGDPAAIGVLDLDVLPGGYEPSSWSVDRLHPSERGHRMLAAGFAALL
                     ACAGFAVPHPVSLECAGGREVTAVQRAAWLVVKGVPWLVRRGRDLGPVILQGLAGELL
                     RMAR"
     gene            complement(13493..13705)
                     /locus_tag="VFE39_14020"
     CDS             complement(13493..13705)
                     /locus_tag="VFE39_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259760.1"
                     /translation="METHTCPLCSLPQTPADAAGLTWSSQHEPDGSTTWICPTCTRAQ
                     LWRIEAMLAVASPTAPIAPVPLRRAA"
     gene            complement(13780..14946)
                     /locus_tag="VFE39_14025"
     CDS             complement(13780..14946)
                     /locus_tag="VFE39_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6259761.1"
                     /translation="MKELNGVIAACPPSGIRRFFDIAAEMDDVISLGVGEPDFVTPWH
                     VREAGIYALEHGYTTYTSNAGLPRLRELICADLASRYGATYEPDGECLVTTGVSEGLD
                     LTLRVLLNPGDEVIVPEPCYVAYAPCVAFAGGIPVSVPTRWEDGFAVDADVVAAAIGP
                     RTKAILIGSPANPTGAVQPRAALEALVRLAEQHDLYLVSDEIYSRLTYSSGHTCLGTL
                     PGARERTVLLGGFSKAHAMTGWRVGWICAPAPVAELCVRVHQYTMLCAPHVSQLAAVE
                     ALSGPDDAVQVMIADYDRRRRVFVKGLREIGLDCPEPEGAFYAFPSIRSSGLDSETFA
                     EQLLRTERVAVVPGSVFGPSGEGHVRCSYATALPLLEEALRRIDRFLRRSAGPR"
     gene            complement(14943..15371)
                     /locus_tag="VFE39_14030"
     CDS             complement(14943..15371)
                     /locus_tag="VFE39_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HET6259762.1"
                     /translation="MTGLPVAEVSEAIATWERSGAIRRYKAVVDWDAVDEDPTSETVT
                     AFIDVSLAPARGVGFDDMAARIARFDEVRSVHLVSGGHDLRCTVTGRTIRAVSDFVSQ
                     KLSTIDRVQATATHFVLKTYKRDGDSFTPPEQDHRLPVTP"
     gene            complement(15368..16636)
                     /locus_tag="VFE39_14035"
     CDS             complement(15368..16636)
                     /locus_tag="VFE39_14035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET6259763.1"
                     /translation="MSQPVHLSARLREVRGSAIRDLLALTARDDVISLAGGLPGTDLL
                     PRERIATATASALADATVVQYGETSGLRRLRAVVAAHETERIGRAIAPDDVVITSGSQ
                     QALDLVARATLDPGDTVVVEDPVYVGALQVFQAAGAALQPVPVDAAGMDLDVLTELLA
                     AGLRPRLVHTVSSFQNPRGVTLAGPRRARLAALADRYGFLVVEDDPYGLIAFDGAPPV
                     PVAAHGERVVRLGSASKVLAPALRVGWLTGPPVLAAAVERLKQCTDLCGSTLTQAVAA
                     DLLADTAWFQAHLRVLRTALRGRAVALSAALHDAFDGAVTCSTPAGGMFCWVEFTDGT
                     QPADLLPHALRHGVGFVPGSAFAVGRPVDHAARLCFASSPEPVLRTAVHRLAAAWREQ
                     RALRRNVEACGRSSNCWSATPSSPTTPSRP"
     gene            complement(16846..17157)
                     /locus_tag="VFE39_14040"
     CDS             complement(16846..17157)
                     /locus_tag="VFE39_14040"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET6259764.1"
                     /translation="MVRIYAQVHLDLLLLAVISSEPGDGAAVMRELRDWSGGRFAPSP
                     RTVYGALNHLERNRLIRRSYVEPRRYKLTDSGRRSLHTRRKEWESFATGVYAVLQRAR
                     T"
     gene            17217..17534
                     /locus_tag="VFE39_14045"
     CDS             17217..17534
                     /locus_tag="VFE39_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259765.1"
                     /translation="MLLIILMFAGSRAQGSRAGRDGPWRTPPLPGRRAEAGRVIGPLG
                     YGAGEQRAPLAGVGVRHRVAAAQHHGQRTRVAPCVGARMVPRCTDISGRFGGSDAAMA
                     RWW"
     gene            17534..>18557
                     /locus_tag="VFE39_14050"
     CDS             17534..>18557
                     /locus_tag="VFE39_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6259766.1"
                     /translation="MSTEADGLFELDEPAPAAASEYPVAGERARATAPLAVRMRPRTL
                     DEVVGQAHLLRPGAPLRRLVEGGAAASVLLYGPPGTGKTTIARLLAGVGHRHFVALSA
                     LSSGVKELRAVIDDARRRRDRLDQQTVLFIDEVHRFSKTQQDALLGAVEDRLVLLVAA
                     TTENPSFSVVSPLLSRSLVLQLQSLTEDEVRELLRRAVGDQRGLNGAVELAQDAEDAL
                     VRLAAGDARRGLTALEAAADGVLGTGSTVIDVPAVERAVTEATVRYDRQGDQHYDVIS
                     AFIKSIRGSDPDAALHYLARRRLLAGEQTVLFIDEVHRFSKTQQDALLAAVENRVVLL
                     VGATTEN"
BASE COUNT         2535 a         6563 c         6848 g         2611 t
ORIGIN      
        1 gcccgacctg cgacgtcgga caggtcctag tctggtgggg tgctcgtcgc agggtttccc
       61 gccggttcgt tccagaccaa ctgctatgtc gtagcagcgg atgcgggcga ggcgtgcgtg
      121 gtggtcgacc ccggccagga cgccgtcgaa cccctcgagg cactgctcgc cgagcaccgg
      181 ctcaccccgg tggcggtgct gctgacccac ggtcatttcg accacacgtt cagcgtcgcc
      241 cccgtgtgtg acggccacga cgtcccggcc tggatccacc ccgaggaccg gtcaatgctg
      301 gccgatccga tgaagggcat ctcggcggag gcggcggcgt tcttcggcgg ccggatggag
      361 ctgcgcgagc cccgcgaggt acgcgagctc accgacggcg cggcgctcga catcgccggg
      421 ttcacgctgc gcgtcgacca cacgccgggg cacacccgcg gctccgtcgt cttcacaacc
      481 cccaccgagg agggcgtcga ggtgatcctc gccggcgaca cgctcttcgc cggctcgatc
      541 ggccgcaccg acctgccggg cggagaccac gcgcagatgc tcgcgagcct gcgcgacaag
      601 ctgctcaccc gcgacgactc cgcggtcgtg ctgcccgggc acgggccgac caccaccgtc
      661 gggcgcgaac gggcgtcgaa cccgttcctg cagggtctcg aattgccgac caccgggcgc
      721 gggctgtgac ggccgcgccg agcgccagcg gagcccgttc cgacgccgtg gagcggcgcg
      781 tgtcggcctt cgcggccccg aagggcatcc cggagtacgt gccaccggac tcggcgggat
      841 tcgagcacgt ccacaacacg ctggtcagcg cggccgatcg ggccggctac gggctcatcg
      901 agctgcctgt cttcgaggag accgcgctct acacgcgcgg cgtgggcgaa tcgaccgacg
      961 tggtcagcaa ggagatgtac accttcgccg accgcgggga acggtcggtc acgctgcgcc
     1021 cggagggcac ggccggggtc gtccggtcgg tgatcgagca cggcctcgac cgcgcaggcc
     1081 tgccggtgaa gctgcgctac gccggcccgt tcttccgcta cgagcgcccg caggccgggc
     1141 gctaccggca gctgcagcag gtgggcatcg aggcgatcgg cgtagacgac ccggccctcg
     1201 acgccgaggt catcgccgtc gccgacgagg gtttccgcgc cctgggcctc accggctacc
     1261 gcctggagat cacctcgctc ggcgacaccg agtcccgccc cccgtaccgg gccatgctgc
     1321 aggagttcct ggccgggctc ccgctggacg aagccacgcg gatgcgggcg gagatcaacc
     1381 cgctgcgggt cctcgacgac aagcgcacgg aggtgcgcgc gctgctgtcg gacgccccgt
     1441 tgctggtcga ccatctctcc gcggcgtcgg ccgagcacca cgctgcggtg aagcagcacc
     1501 tcgacgacct gggcgtggcc tacgtggaga acccgcggat ggtgcggggt ctcgactact
     1561 acacgaagac gaccttcgag ttcgtgcacg acggcctcgg cgcccagtcc gggatcggcg
     1621 gcggcgggcg ctacgacggg ctgatggcca cgctcggcgg gcagccgctg tccggcgtgg
     1681 gcttcggcat cggcgttgac cggacgctgc tggcgtgccg agccgaaggg gttgcgccct
     1741 ggtccgaggc gcgctgcgag gtcttcggcg tgccgttggg cgacgcggcg aagcggcggc
     1801 tcgtggtgct ggccgcgcag ctgcgccggg ttggcgtgcg ggtcgacctc gcctacggcg
     1861 gccgcggcct caagggtgcg atgaaggccg ccgaccggtc gggcgcccgc ttcgcgctgg
     1921 tgctcggcga gcgcgacctc gaggccggca cggtgggcct caaggacctg gcgtcggggg
     1981 agcagcagga cgtcgccctg gcggatgcgg ccgacgaggt gctcacccgc ctgcgctgac
     2041 cgggactcgc cctcccgttg cctaggatca tcgccgtggc caccgaagat cgtgagcgtt
     2101 gggaggcccg gcacgccgcg gtctcctcgg cgggcgcacc ggacatcccc atgccgcccg
     2161 acgcgctccg cggccgcgag cgcctgctgc ccgacggcgg acgcgcgctg gatgtggcat
     2221 gcgggcgggg caccgtggcg ggatggctcg ccgagcgcgg gttcgtcgtc gacgcggtgg
     2281 acatctcgga ggccgctgtg gccgcgggcg cggcgctggt ggttgaccgc ggtgtggccg
     2341 agcgggtgcg ctggtggcgc tatgacctgg acgccgggtt gcccggcggc tgcgccggac
     2401 cgtacgacgt cgtcgtgtgc cagcggttcc gcgacccggc gttgtacccg gtgctcgcgc
     2461 agcgcctcgc ccccggtggc ctgctggtgc tgacggtgca gtcggtggtc ggggacgagc
     2521 ccggtcggtt ccgcgcgtcc cccggggagc tgctcgccgc gttcgccgag ctgacgacgc
     2581 ttcaccacac cgagtccgac ggccaggcga gcctcctggc gcgccgccgc tgagcgcacc
     2641 tccgccgcgg ttgccgagtc ggacgaacgg cacgcaggtc gatgggacgg aagctccgtt
     2701 ggtgtcggct tcatcagcga ttcgcccagg ccgcgccctt cagactgtgc ttccggactc
     2761 gcggctcgcc accatggcgg cgagtgcggt ggtgatcggc acggaggcca ccagcccgat
     2821 gctgccgacc agcgtgcgta ccacctcggt ggccacgtcc tgggtggtca cgacgtcccc
     2881 gagtgcgcgg ccggacagcg tgaacagcag gagcagcggc agcgatgctc ccgcgtaggc
     2941 cagcaccagg gtgttgacgg ccgacgcgac gtggtcgcgc ccgatccgca tcgccgcgcc
     3001 gaagagagcc cgagctccca gctccgggtt ggcccggcgc agctcccaca ccgcgctggt
     3061 ctgggtgacg gtcacgtcgt cgagcacgcc gagagccccg atcaggacac cggcgagcag
     3121 aaggccgcgg gcgtcgacgc cggtgccgag cgtggcgatc aggttgctgg tctggtcgtc
     3181 gaggccggtg agccgggccg cgacggagaa cgcggccccg aggcccccga tcagggcgag
     3241 cgacaggagc gttccgagga cggccgtcga cgtgcgcgcc gagagtccgt gcgtcaggta
     3301 caggaccacg aacatgatca ggcaggagcc gaccactgcc acggcgagcg ggtcgtgccc
     3361 ggccaggatc gagggcagca cgaacagcaa cagaacagcg aagctcagca ccagcgccgc
     3421 gagcgcggcc aggccccgcc agcggccgag caggagcacc gctcccgcga agatcgcggc
     3481 gagccagccg agcggcgcgc cgcgctggaa gtcgacgacc tggtacgacc cggggtcgtt
     3541 cgggttggcg ccggaccagc ccagcaccac ccggtcgccg acggcgaagg tcggcgatcc
     3601 gggttcgaca ggcaccacct ggacgaggtc gcgccctggc atcggcccgt cggccatccg
     3661 gacgacgagc gcgacgcagc tgccgtcgcc cgaacctgca ctgcagtcgg acgcactggc
     3721 ggagaccact tcggcgtgca cgggctgttg ggtgacgggc tgggccgtgg tcgggggacc
     3781 gccggccggc cacaacagaa ccacgccggc gagcgtcgcc agcgcgcacg ggacgagcag
     3841 tgtggcgatg agcagccgca cccgcccccc cgcaggcggc gccggcgagt gaccgtgccc
     3901 gtggccatga gcgggctcac gggccgaggc ggtggaggtc gcaaccgcgg cctgcggtcc
     3961 ggaacggacg acccggtcgg cctgttcctc gtcccggtcc ccctggccgg gggaccggcc
     4021 cgccgtacgc gggccgcggg cggcagcggt gcgggaggcg ctcgtgcgca cggttcgtgt
     4081 gtcgaccggc gtccacccgg tcggcggcgt cggggcctcg gctgccgcct cgtcggcgtc
     4141 atcgagttgg cccatgcggt ccggatgccc gtgccggccc gagcccggcc ggggcggcag
     4201 cgggaacgcg gcgggcggcg gggtcgtggc agcaccggcg gaccggccga accggtgccg
     4261 tgccgtgtcc cgccccgcgg tcccgggctg ctcggatgcg ggtggaagcc ccggctcgtc
     4321 agtcggcacg tgcccatcct gtcaacggcc cgccgcccga cccgccctgg tccaccggac
     4381 gccctccacc cggcgggagc tgatcagcgg gaacgatcat ccgcagcttc ctccgacgtg
     4441 tcgccgccca gctgttgcag cgcccgctca gcgacggcgg cagcatcgcc cgtggtgtcg
     4501 acgccgatcc cgggctcgtc gggcccgagc ggctcgagcg cagccagctg gctgtccagc
     4561 agggacggcg gcatgtagtg gttcctgcgc gcggtgaccc tggccgcgat cagctccggc
     4621 ggcgcgagca ggtgcacgaa ccgcacggag gggtgaccgt cctgcagcag ctcgcgatag
     4681 gagcgcttga gcgccgagca cgtgaccacc gcactgcggt cggccgcctc ctgctccccg
     4741 atccaggcgg ccacccggcg gagccacggc cagcggtcgt cgtcgtccag cgggatgcct
     4801 gctgccatct tcgcgcggtt gctctcgggg tgcaggtcat cgccctcgac gaacacccat
     4861 cccgtacgtc ggacgagctc ggcggccacg gagctcttgc ccacgcccga tacgcccatc
     4921 accaccaggg tcaccgtcat ggaggcagac tggcgcatgc ccccgacagg gagttgcccc
     4981 gggtgtcgaa catgtgttcg aatactgcca tgcggtggag cggacagcag gtgctcgacg
     5041 acggcgtcgg tgcggaggcg gcgctgccgg ggttgcgcgg gctcctgcgg tcggtgcgca
     5101 ctcccgagtt cgccggggcg gtgttccacg aggtggaggc caaatcggtg ctcaaccggg
     5161 tgccgggcag ctcgccgatg ccgttccgct ggacggtcaa cccgtaccga ggctgctccc
     5221 acgcctgcgt gtactgcctc gccggcccca cccgcgtgct gttggccgac gggcgcaccc
     5281 gccccatcgc cgagctgcgc gtcggcgacg ccgtaatggg cacggagctg gtcgacgggc
     5341 agcgtcgcta cgtacgcacc accgtgcggg cgcactggtc caccaccaag ccggcgcacc
     5401 gcctccggct cgccggcggc agcgaactgg tcgccagcgg ggagcatcga ttcctcaccg
     5461 accagggctg gcgtcacgtg agtggtggct ggtgccgctc cgggcggcgg ccgcggctgc
     5521 gcactggcag cgcactgctc ggccccggtc cggcggccat ggtcgggtgg cagcgccgcc
     5581 cccacaccgc ggcctaccgg cagggctacc tgtgcgggct ggtgcggggc gacggtctcg
     5641 gcgccgacgg ggagaggtgc ttcccgtccg agcatctgga gctcgaggcg ctgggtcggg
     5701 cccaccattt cctcgccgag gcggcccagc acgtcgttga gctgccgtcg gcggtcccgg
     5761 tcccggtccc ggtcggcgcc ggggagcccg gccggatccc ggcccccacc ccccgcggcg
     5821 ggatcgcgcc gatcaccgac gtggtgcggt ggcccgagca gcccggcgac gactggtgcg
     5881 cagggttcct cgccggcacg gtcgacgccc gcggctcggt cgtgcggggc gagctgcggg
     5941 tccgccacgc cggcgaggag atcgccgggc gggcagcacg cgcgctgcac cgcctcggct
     6001 tccggttcgc ggtcgaggct gaccacggct ccggtccggg gtcgagcgcg gggcggacgg
     6061 tccggctgct cggcgggtcc gcggcgctgc tccggctgct gcaggtggtc gatcccgcgg
     6121 tcagcaggca ccgagacgtg ggcggtgccg ctgtcaccgg cggcaccgcg ctggtggtgg
     6181 agtcggtgca accgctcggc gtcgagatgc cgatgtacga catcaccacc ggcaccggcg
     6241 acttcgtggc ggagggcgtg gtcagccaca actgcttcgc ccgcaacacc catacctatc
     6301 tcgacctcga tgccggggcc gacttcgaca gccagatcgt ggtgaaggtc aacgtggcgc
     6361 gggtgctcga gcgcgagttg cgctcggcga agtgggcgcg ggaacccgtc gcgatgggca
     6421 ccaacaccga cccgtaccag cgtgccgagg gacgctaccg gctcatgccg ggcgtgatcg
     6481 gggcactcgc gcgctccggc accccgttct cgctgctcac caagggcacc gtgctcaccc
     6541 gcgacctgcc gcagctcgcg gccgcggcgg cagacgtccc ggtgggtctc ggcgtctcca
     6601 tcgcgctggt cgaccgctct cttcaacacc ggctcgagcc cgggacgccc tcgccggccg
     6661 cgcggctcga cctcgtccgg cggatcaccg acgccggcct gtcctgcggt gtgatggtgg
     6721 cgcccgtgct gccgctgctc agcgactccg tcgaggccct cgacacgctg cttgcacagg
     6781 tgaagcaggc cggggccaac ggcgcgagcg tgctggcgct gcatctgcgg cccggcacgc
     6841 gcgagtggtt cctggcgtgg ctcgcgcggg agtacccgaa gctcgtcgcg ccctatgccc
     6901 ggctgtaccg ccgcggcgcc tacgtcgacc cggagtatcg gcgcgcgctc ggtgagcggg
     6961 tggcgccgct gctgcgccgc cacggcctct ccggtgccgc caccacggtg cgcgggcagg
     7021 ggtggcctgc ggcgagggcg ccgaccgtcg agactccgtc gggggagcag ctgagcctgc
     7081 tctgaccgcc gtcggcggcc gggctccgag tcgtgacgtc gaccatcccg gaacatgggg
     7141 aaacccttac tcacgcataa ggtgtgagaa cgcgcgacag gcatccacag ttgcagtcag
     7201 acggcatgat tttcgcctca cagatcgggt tgagtacaac gcgtggctcc gctcgtgtga
     7261 accgtaagcc gatgcgcacg gcaacggcgg ctcacactgg ggaggcccga aatgactcgc
     7321 atgagtcgac gcacactgga ggcggctggc gccatcgtcg cggtcgccgg actgtcgttc
     7381 gtggggttga gcgccgaggc agacgccgcc gcgcagccct cgcctgcggc gctgagcggc
     7441 ggcacgggga tgtccatgcc gatggactcg atcggcgcgt acgaccccca gaatcacagc
     7501 gacgccacgt tcttcgcgtc cgtgttgaaa ggcgccaacg aggtgccggt cacgagcgga
     7561 ccgaaggtcg gcgacaagaa cggcagcgcc acggccctga tgcgcatcca gggcgaccag
     7621 gtgtcgtacg ccttcacgtg gacgggcatc ggaacaccga ccatgggcca catccacgag
     7681 ggccccacgg cgaacggaga cgtgcagatc ccgttcttcg gcacgaagct gccggccggg
     7741 cgcaattcgg tgatcggcac ggtaaaggtg accaacaagg atcttctggc gaatctgaag
     7801 acccacccgg agcagttcta cttcaacctg cacaccgcag agttccccgg cggtgcggtg
     7861 cgcgggcagg tgcacgcgct tccggagaag ctcaacctgc aggccgctat gacggccgac
     7921 caagtgcact cggtcgtcag gggcgcccag atctacgcct gcaccaggca taacgacggc
     7981 tcgttcacgt tcacccagaa caacgtggac gccacccttg gtggcggcat caggcacacg
     8041 ttcctcaagg ccggcccagc gggaccgccc cagtgggtcg cgcccgacgg gtccggggtc
     8101 accggcaagg ccctcaccat gatcccgaac ggcggcaaga acatccccga actggtcctc
     8161 gcggcgacgc cggagggcag gcccggtggc gtgctgtcgt cgacgcagat cgtgatgcgc
     8221 ctcaacaccg tcggtggagt cgcccccgct ggtacctgca accccgcggc acagccgaag
     8281 gcagcggtgc cgtacacggc cgactacctg ttcgtcacct ccacgtagcg cacaagcgcg
     8341 tcgaccccct gtggcagtcc gatcgggacg ccaccagggg gtcgaggcct gttgcggctc
     8401 cacggtcgga tctcggtcgt ccacggggac gagcggacgg tgaccgggga aacctgctgg
     8461 caggccgacc gggggcggcg gatagcctcg acatgtgatg cgcacccacc ccgccggcac
     8521 cctgcgtgcc gagcacgccg gacagtccgt gaccctcgcc gggtgggtgg cgcgccgccg
     8581 tgatcacggc ggggtcatct tcatcgacct ccgggacgcc tccggctccg cccaggtggt
     8641 gttccgcgag ggcgagatgg ccgagcgcgc tcaccggctg cgcgccgagt tctgtgtcgt
     8701 ggtcaccggt gaggtcgtcc gccggccggc ggggaacgag aaccccgagc tcgccaccgg
     8761 tgcgatcgag gtcacggcca ccgagctcac ggtgctgtcc gagtcggccc cgctgccgtt
     8821 cccgctcgac gagcacagcg agatcggcga ggagatccgg ctcaagcacc gctacctcga
     8881 cctgcgccgc agcgggccgg cggccgcgct gcggctgcgc agcgaggcca accgcatcgc
     8941 gcgcacggtg ctgcacgacc gggagttcgt cgaggtggag acgcccacgc tcacccggtc
     9001 caccccggag ggtgctcgcg acttcctcgt gccctcacgc ctgcgccctg gctcctggta
     9061 cgccctgccg cagagccccc agctgttcaa gcagctgctg atggtggcgg gcaccgagcg
     9121 ctactaccag atcgcacggt gctaccgcga cgaggacttc cgcgccgacc ggcagcccga
     9181 gttcacccag ctcgacatcg agatgagctt cgtcgagcag gacgacgtga tcgagctcgg
     9241 cgagacaatc gtctcagcgc tgtggtcgga gctcgcgggg tacgagatcc cgcgcccgat
     9301 cccgcggctc acctacgccg acgcgatggc ccgctacggc tcggacaagc ccgacctgcg
     9361 gttcggcgtc gagctcaccg acctcaccga gtacttcgcc gacacgccgt tccgggtgtt
     9421 ccaggccccg tacgtcgggg cggtcgtgat gccgggcggg gcgagccagc cgcgcaagca
     9481 gctcgacgcc tggcaggagt gggccaagca gcgaggcgcc cgtggcctcg cgtacgtgct
     9541 ggtcgacgac gacggctcgg tgagcgagcg cggcccggtc gtcaagaacc tctccgaggc
     9601 cgagcgtgag ggcctcccga aggccgtggg cgccgagccg ggagacgcgg tcttcttcgc
     9661 cgccgggcgc ccagccgacg cgcgcgaact gctcggcgcc gcccgcggcg agatcgcgcg
     9721 gcgctgcggt ctggtcgacg agaacgcctg gtcgttcgtg tgggtcgtcg acgcaccgat
     9781 gttcgagcgg gtccgtgacg acaagggcga cgaggtcggc tggaccgcgg tgcaccaccc
     9841 gttcacctcg ccgaacgagg agtggatgga ccgcttcgaa gaagcgccgg accaggcgct
     9901 cgcctacgcc tacgacatcg tctgcaacgg caacgagatc ggtggcggct cgatccgtat
     9961 tcaccgctcc gacatccagc agcgcgtgtt cgctcttctc ggcatgaacg aggccgagca
    10021 gcgggaaaag ttcggtttcc tgctcgacgc gttccagtac ggcccgccgc cgcacggtgg
    10081 catcgcgatc ggctgggacc gcacctgcat gctgctcgcg cactccgact cgatccgtga
    10141 cgtcatcgcc ttcccgaagt cgggtggcgg cttcgatccg ctcaccgggg cgcccgcgcc
    10201 gatcacacct gagcagcgca aggaggcggg cgtcgacgcg cagccgcctg cggcgcccgc
    10261 cgctgcgtcc ccggctgccg acgagccgcc caccaagctc tgaacgcgcc tccgtacctc
    10321 cggccgacgc ggggagcccg tccgtgctgc acatgagggt gatctcgccc gtgggccaga
    10381 ccgacgcggt gcgggacctg ctgctaggcg aacccggtgc cacgcacgtg accgtcctgc
    10441 ccgggatcgc gctgcaaccc cccggtgacg tcgttgaggc cgacgtggcg cgggagtcgg
    10501 tggacccgct tctcggcgcg ctgtccgagc tgggtgtcga tcgcgccggc ggcgtcaccc
    10561 tggaggtgat cgacacctcg ctctccgacg ccgctgacgc cgcggaggag gcagcaccgg
    10621 gcgacccgtc ggacgccgtg atctgggacg agctggtggc gcgcaccggt gaggagtcgc
    10681 ggctgtccgt gagcttccag gccttcctca cgatcgcgtg cctgctggcg gccatcggcg
    10741 ccatcacgaa ctcgccggtg acggtggtgg gtgcgatggt gctgggtccc gagttcgggc
    10801 cgctcgccgc gatcgccgtc ggcgcggtgc tgcgccgggg agatctggtg cgccggggcg
    10861 gcttggcgct cgccgtcggc ttcccgctgg cgatggtgat cacggcgctg gcggccgtgg
    10921 gtttcgacgc gaccgggctc ctgtccaccg cgaccctcga caacctcgat caggtcgact
    10981 tcatctacaa ggtgggaccg ttctccttca tcgtggcgct gctcgccggt gccgccggga
    11041 tgctcgcgct gacatcggcg aagtccgcct cgctggtcgg ggtgttcatc tcggtgacca
    11101 ccgtgcccgc cgcggcgttc gcgtcgaccg ctctcgtgga ggggcgttac gcgcaggccg
    11161 ccgggtccgc gctgcaactc ctggtgaatc tcgtgggcat cgtcgtggcg gcgacggcgg
    11221 tgctcctcgt gtcgcatcgt acggctgtcc gtgagcgggg tatgcgccgg ttgtcggcgg
    11281 ggtagggtcg cacacgatgg ttcgcgacct gggcagtttc cccaggccgg tgctcgccgc
    11341 gctcgctgct gccgagcggc tgctcagccg ccggtccggt gccggcatcg tgctggccga
    11401 ccctgaggat ctcgggggca gtgaccggtc ggtggtggca cgtgcgcgcg tggcccgaaa
    11461 tcccttctcg ctgccgcgca cgctcgtcgt caagcactat ctcgggcggc cggatccgga
    11521 ccgccccgac ccgttccact acgaggttgc cagctgccag ctgttcaccg ccctgcctgc
    11581 cgaggcccgg cccagtccgg tcctgatcgc gcacgacccg cagagccggc tgctggtgct
    11641 cgaggacctc ggccgcagct cgacgctcgc cgacaagctg ttcggccccg accccgccgc
    11701 cgcccagcgc tgtctcctca gctgggctcg tgcgctcggc cgaatgcaag ctgcgacagc
    11761 gggtcgcgag aacgatttcg gggcactcct gcgcaggctg ggggagcggg cgtggcgcga
    11821 cccgatggcc gaggacgctc gtgtggcgct ggccgatgtc ccggggctgc tggagcagga
    11881 tctcggggtg acggtgtcac cagcggcctc actggaggcc caggagaccg cgcggctgct
    11941 ggggggcacc cggtaccgcg cgttcagccc gtcggacacc tgcccggaca acaatctcgt
    12001 caccagccgc ggagtccggt tcgtcgactt cgaatgggga tgcttccgcg acgtggcgct
    12061 cgacgccgcc tacttccgcg tgcccttccc cggctgcgaa gccagcttcg cgatgccgcc
    12121 cggaatggcg gagacgatgc tcgaggcgtg gcgtaacgag atcgccgccg tctggccgga
    12181 tctcgacgag ccggaccgac tcgaccgcag gctgctgcac gcgcagctgc tgtgggtgtg
    12241 gctgtgcacc tggtggttgt taccccggat ccgcgtgcgg gacatgccgg tggggctcga
    12301 cgccacccga tccccccgga tcagcaccgc gttgggccac tactggcggg agatggcacg
    12361 caccgccgcc acgggcggcc tgccggccac ggccgagctc ggcacgatgg tcgccgacgc
    12421 gctggcgaaa cggttccccg acgcacccgg ctcgcttccg gtgttcccgg cgttccggtc
    12481 ggtcgcctga gaccaccggg agcggtgcag gtcgggcaat gctggtcagg caccgatcgt
    12541 tcccccacct gcccacgggg cgtcatccgt cgcacacccc tggtgagccg agggcggtga
    12601 tggtgtcagc gtgaccgtgc agcccctgct cgcacccctc gcgactgcgc ccatcacgcc
    12661 cggcatgtcc accgcgccgg ccggaacccc gcaggtgggg acattggtga cactcggcga
    12721 ctccacctcc gtcgggctcg gcgaccccgt gcccggcggg ggctggcgcg gtttccccgt
    12781 gctgctgcgc gatgcgctcg gcgcgccgac actggtcaac ctcggtcgca cgggagcccg
    12841 catggcttgc gtgcgcgacg agcagctgcc ggtggcgctg ctggctgcgc ccgacgtcgc
    12901 ggtgttgttc gtcggcatga acgacaccct gcgctcagac ttcgaccccg agacgctgca
    12961 cgcccactgc tccgcggtcg tcggcgcgct gcgggccggt ggggcgcacg tcctgatgct
    13021 gcgctaccac gaccacaccc aggtgttccg cctgccggct ccgctgcgac gggccctgta
    13081 caggcggatc gtggcgctca acgccgtcac cgatgcagtg gccgccggcg acccggccgc
    13141 gatcggagtg ctggatctgg acgtgctgcc cggcgggtac gagccgtcgt cgtggtcagt
    13201 cgaccggctg catccctcgg agcgcgggca ccggatgctc gcggcagggt tcgcggcgtt
    13261 gctggcctgc gccgggttcg cggtgccgca cccggtgagc ctggaatgcg cgggcgggcg
    13321 cgaggtgacg gcggtgcaac gggcggcgtg gctcgtggtg aagggcgtgc cgtggttggt
    13381 gcggcgtggg cgcgacctcg ggccggtgat ccttcaggga ctggccggcg agctgctgcg
    13441 gatggcccgc tgatccccat gggggtcgac ttgagtccgg cccatcggtt ggtcaggcgg
    13501 cgcggcgcag cgggaccggg gcgatcggtg cggtggggga ggccacggcg agcatggcct
    13561 cgatccgcca cagctgcgcg cgggtgcacg tggggcagat ccaggtggtg gagccgtcgg
    13621 gctcgtgctg gctgctccag gtgaggccgg cggcgtcggc cggcgtctgc ggaagggaac
    13681 agagggggca ggtgtgagtc tccatgcctc ctgagtctgg cgcaccggct ttgcgggcct
    13741 gtgaacccga cgcaaacgat gcactccatg gacgtccgac tagcgggggc ccgcagagcg
    13801 acgcaggaaa cggtcgatcc gcctgagggc ctcctcgagc agtggcagcg cggtggcata
    13861 ggagcagcgc acgtgtccct cgccggacgg gccgaacacg ctcccgggca ccaccgcaac
    13921 gcgctcggtg cgcagaagct gctcggcgaa cgtctccgag tccagccccg aggaccggat
    13981 cgacgggaag gcgtagaagg cgccctccgg ctcggggcag tcgaggccga tctcgcgcag
    14041 gcccttcacg aacacccggc gccgccggtc gtagtcggcg atcattacct gaacggcgtc
    14101 gtcagggccg gacagcgcct ccaccgccgc gagctgcgag acgtgcggcg cgcacagcat
    14161 cgtgtactga tgcacgcgta cgcagagctc ggcgacgggc gcgggcgcgc agatccagcc
    14221 cacccgccaa cctgtcatgg cgtgggcctt cgagaacccg ccgaggagca ccgttcgctc
    14281 ccgggcgccg ggcaacgtgc cgaggcaggt gtgcccgctg ctgtaggtga gcctgctgta
    14341 gatctcgtcg gaaaccaggt agaggtcgtg ctgctcggcg agccggacca gcgcctccag
    14401 cgcggctcgc ggctgcacgg ccccggtcgg gttggcgggg gagccgatga gtatcgcctt
    14461 ggtgcgggga ccgatcgccg cggcgacgac gtcggcgtcg acggcgaagc cgtcctccca
    14521 gcgtgtcggc actgacacgg ggatgccgcc ggcgaacgcg acgcacggcg cgtacgcgac
    14581 gtagcagggc tccggcacga tcacctcgtc gccggggttg agcagcaccc gcagggtgag
    14641 gtcgagtccc tcggagactc cggtggtgac caggcactcg ccgtccggct cgtaggtggc
    14701 gccgtagcgg gaggcgagat cagcacagat cagctcgcgc aggcgcggca gcccggcgtt
    14761 ggacgtgtag gtggtgtagc cgtgctccag ggcgtagatc cccgcctcac gtacgtgcca
    14821 cggcgtcacg aaatccggct cgccgacgcc gagtgagatg acgtcgtcca tctcggcggc
    14881 gatgtcgaag aagcgccgga tgcccgacgg cggacaggcg gcgatgacgc cgttgagctc
    14941 cttcacgggg tcaccggtag ccgatgatcc tgctcgggcg gtgtgaagga atcgccgtcg
    15001 cgtttgtagg tcttgagtac gaagtgggtg gccgtggcct gcacccggtc gatggtggag
    15061 agcttctgcg agacgaagtc cgacaccgcg cggatggtcc gcccggtcac cgtgcagcgc
    15121 aggtcgtggc cgccggagac gaggtggacg ctgcgcacct cgtcgaaccg agcgatccgg
    15181 gcggccatgt cgtcgaaacc cactccacgc gcgggtgcca gcgagacgtc gatgaatgcc
    15241 gtgaccgtct cgctcgtcgg gtcctcgtcg accgcgtccc agtcgaccac agccttgtag
    15301 cggcggatcg cgcccgatcg ctcccatgtc gcgatcgcct cgctcacctc ggcgaccggc
    15361 aacccggtca tggtcgcgat ggtgtcgtgg gagagctggg cgtcgcgctc cagcagttcg
    15421 aggatctgcc gcacgcctcg acattacgtc gaagggctcg ctgctcgcgc caggccgcgg
    15481 cgagccggtg caccgcggtg cgcagcacgg gctcggggct cgacgcgaag cagaggcgcg
    15541 cggcatggtc caccggccgg ccgacggcga aggcgctgcc cgggacgaac ccgacgccgt
    15601 gccgtagcgc gtgcggcagc agatccgcag gttgggtccc atcggtgaac tccacccagc
    15661 agaacatgcc gccggccggg gtcgagcagg tcaccgcgcc atcgaacgcg tcgtggagtg
    15721 cggcactgag ggcgacggcc cggccgcgca gcgcagtgcg cagaacccgc aggtgcgcct
    15781 ggaaccaggc cgtgtcggcg agcagatcgg cagcgacggc ctgggtgagt gtcgagccgc
    15841 acaggtcggt gcactgcttg agccgctcca ccgcggccgc gagcacaggc ggaccggtga
    15901 gccagccgac gcgaagggcg ggcgcgagca ccttcgacgc gctgccgagc cgcacgaccc
    15961 gttcgccatg ggcggcgacc ggtacggggg gtgccccgtc gaaggcgatc aggccgtagg
    16021 ggtcgtcctc cacgacgagg aagccgtacc ggtcggcgag cgcggccagc cgggcgcgac
    16081 gcgggccggc gagtgtcacc cctcgcgggt tctggaagct gctgacggtg tggaccagcc
    16141 gcggacgcag accggcggcc aacagttcgg tgagcacgtc gaggtccatg ccggcggcgt
    16201 cgaccggcac gggctgcagc gccgcgcccg cggcctggaa cacctgcagc gctccgacgt
    16261 agacggggtc ctcgacgacc accgtgtcac cggggtccag cgtcgcgcgg gccacgaggt
    16321 cgagagcctg ctgtgagccg ctggtgatca cgacatcatc gggtgcgatg gcgcgcccga
    16381 tccgctcggt ctcgtgggcc gcgacgacgg cccggagcct gcgcagcccg gacgtctcgc
    16441 cgtattggac gacggtcgcg tcggcaaggg cggacgccgt cgcggtggcg atccgctccc
    16501 gcggcagcag atcggtgccg ggcagcccgc ccgcgaggct gatgacgtcg tcgcgtgcgg
    16561 tcagggccag caggtcacgg atcgcggatc cacgcacttc acgcagccgg gcggacaggt
    16621 ggacgggctg ggacacgggg gctcgccttc gtggtcggtc cgggacgggc cgggacggcg
    16681 atgacgcccg gcctgcgatc gtcctgggtc gacagggcga ggggggcgag cccggcttgt
    16741 ggcgcgccct cctcaggatg ccgcatcgtc ctgcgatgcg gaacttcttc tcataatgtg
    16801 ggaagcgcgg accactcgta cctcacccgt gtcgccggct cgccgtcacg tccttgcgcg
    16861 ctgaagcacg gcgtacaccc ccgtcgcgaa ggactcccat tccttccgcc tggtgtgcag
    16921 cgagcggcgt ccggagtcgg tgagcttgta gcggcgaggc tcgacgtacg agcgccggat
    16981 gagccggttg cgctcgaggt ggttcagcgc cccgtacacg gttcgggggg aaggggcgaa
    17041 cctcccgccg gaccaatcgc gaagttcgcg catcaccgcc gcgccgtcac ctggctccga
    17101 gctgatcacg gcgagcagca gcagatcgag gtggacctgg gcgtatatcc gaaccatctc
    17161 gagcctctcc atcgcggtat gacgtcggaa cttccccgcg ccggagcgag gtgaacatgc
    17221 tgctgataat cctgatgttc gcgggctcgc gagcgcaggg atcgagggcg ggacgtgatg
    17281 gaccgtggcg caccccgcca ttgcccgggc ggcgggcgga ggcgggccgg gtcatcggtc
    17341 cactgggcta cggagccggt gaacagcggg cgcccctcgc cggcgtcggc gtccggcacc
    17401 gcgtcgccgc cgcgcagcac cacgggcagc gaacccgcgt cgcgccctgc gtcggcgccc
    17461 gcatggtccc acgctgcacc gacatctccg ggcggttcgg tggttccgac gccgcaatgg
    17521 cacgctggtg gtgatgagca ccgaggcgga cgggctgttc gagctggacg agccggcgcc
    17581 ggccgccgcg agcgaatacc cggtggccgg ggagcgggcc cgtgccacgg cgccgctcgc
    17641 cgtccggatg cgcccgcgca cactcgacga ggtcgtcggg caggcccacc tcctgcgtcc
    17701 cggcgcaccg ctgcggcggc tcgtggaagg cggagcggcg gcgtcggtgc tgctctacgg
    17761 accacccggc accgggaaga cgacgatcgc gcggctgctc gccggggtcg gccaccgcca
    17821 cttcgtcgcg ttgtcggcgc tgtcgtcggg ggtcaaggag ctccgggcgg tgatcgacga
    17881 cgcccgccgc cgccgcgatc ggctcgacca gcagaccgtc ctgttcatcg acgaggtgca
    17941 ccgcttctcc aagacccagc aggacgcgct gctcggcgcc gtcgaggacc ggctggtgct
    18001 gctcgtggcc gccaccacgg agaacccgtc gttctcggtg gtctcgccgc tgctgtcacg
    18061 ctcgttggtg ctgcagctgc agtcgctcac ggaggacgag gtccgcgagc tgctgcggcg
    18121 ggccgtcggc gaccagcgtg ggctgaacgg tgcggtggag ctcgcgcagg acgccgagga
    18181 cgcgctggtc cggctcgcgg cgggcgacgc gcggcgaggg ctcacggcgc tggaggcggc
    18241 ggccgacggt gtgctcggca ccgggagcac ggtgatcgac gtgccggccg tggagcgggc
    18301 ggtcaccgag gcgacagtgc gctacgaccg tcagggcgac cagcactacg acgtgatcag
    18361 cgcgttcatc aagtccatcc gcggctccga cccggacgcg gccctgcact acctggcgcg
    18421 gcggcggctg ctggcgggcg agcagaccgt gctgttcatc gacgaggtgc atcgcttctc
    18481 caagacccag caggacgcgc tgctggccgc ggtggagaac cgggtggtgc tgctggtcgg
    18541 cgcgacgacg gagaatc
//
LOCUS       DASRSC010000283         5007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3549829,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000283 DASRSC010000000
VERSION     DASRSC010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3549829"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VFE39_14055"
     CDS             complement(<1..696)
                     /locus_tag="VFE39_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6259767.1"
                     /translation="MGTDVLLTSRDGGVLTLTLNRPGRKNAINADLWSALRENLTAAG
                     NDPAVRAIVITGAGGDFCSGADLSGGVAAGHPLGRMRGINEVALLLHELPVPSVAKVD
                     GVAVGAGWNLALGCDLVVCSPGARFSQIFARRGLSLDFGGSWLLPKLVGLQQEKRLAL
                     LAEMIDAAEAHRLGLVTWVVEAEEIDAFVTDLGKRLAAGPPVAIAQSKALLNEGADRT
                     MRDALASEARTQTI"
     gene            complement(710..1819)
                     /locus_tag="VFE39_14060"
     CDS             complement(710..1819)
                     /locus_tag="VFE39_14060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013225683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6259768.1"
                     /translation="MKLVLEPEQQELRSTVRKFLADRSPSAKVREAMASDRGYDADLW
                     NRMAGLGVLGLVVPEELGGAGAGQVERSVVLEELGRALTPSPFLASAVLATDVLLAVD
                     DDAARTELLPRLAAGELVGTVAVGEGGAGWDRQGGATTATARGGSWELAGTKTLVLTG
                     DTADLVLVYASTPDGPGWFAVDGDAAGLIRTVLTGLDPTRRLARLTFAATPARPLVAA
                     DPAAAFDLVADLAAVALAAEQVGGLQRAMELTVDYAKVRVQFGRLIGSYQAVKHGCAE
                     MYSAWEHAVSVLRYAAWVADHDRAELPLAAALAQTYIGPAYFAAATDMVQYHGGVGYT
                     WEHDAHLYYKRAKSSELLFGGPALHRTRLADRLGV"
     gene            complement(1816..3036)
                     /locus_tag="VFE39_14065"
     CDS             complement(1816..3036)
                     /locus_tag="VFE39_14065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6259769.1"
                     /translation="MIMVDATQDYRSSVRAWLTAHAPGPAAGLTSDEQLRRAREFQAA
                     LYDAGYAGITWPKEVGGQGLGTAEQQVFSEEAADFELPTYPFIIGMGMCGPTLVDLGT
                     PEQKHRYLPRLLCGEEIWCQLFSEPGAGSDVASLQTRAVRDGDGWVVNGQKVWTTNAQ
                     WADYGALLARTDPDRPKHAGLTMFIVDMRAPGVTVRPLRDMSGRAPFNEVYFDDVRIP
                     DDGVIGEVNRGWQAAVAMLGHERVSIGATRRPRSNPLEFASLVELARHEGVAADPARR
                     ERLAELYAHERALELFNARMRQETAAGNPPGVRGSVAKLAGALLLRRAIETASDLAGA
                     DAVAWDPDDARAAELALGINSAPASSIAGGTNEVQRNIIGERILGLPKEPQVDRDLPF
                     KEIKVGTQRQGGVR"
     gene            3153..3803
                     /locus_tag="VFE39_14070"
     CDS             3153..3803
                     /locus_tag="VFE39_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6259770.1"
                     /translation="MSTASDLPPGAVDTGAGPSGTPVIPRGGGRDAITGAAREIFAER
                     GYHGASIRDIAKRAGLSLSAMYYWHPSKQHLLAALIEESTYDYFHRCDQALREVGDDP
                     ADRLRALVRATVEYRVRRRVESNIGTQEWRNLEPDNRERLDGLRRSASQLWADIINDG
                     VARGTFRCAHPDDARRSIVAACNAIAQWYEPSGELGLPDLVERYTEIALRIADHRS"
     gene            3884..>5007
                     /locus_tag="VFE39_14075"
     CDS             3884..>5007
                     /locus_tag="VFE39_14075"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET6259771.1"
                     /translation="MAEAYIVGAVRTPIGTRKGALAGVHPADLGAHVLRELVDRTGID
                     PSAVEDVIMGCVVQLGPQAGDIARTAWLSAGLPQSVPGVTIDRQCGSSQQALHFAAQG
                     VMSGTQDLVVAAGVENMAMVPMGTNAAVSKELGASPKGDGWAERYGDQEISQFRGAQL
                     ICEKWGYRRDDLERFALESHRRAVRAIDEGRFAGQITPVAGTDTDEGARRDTSLEQMA
                     QLKGLREGWEITAAVSSQMSVGAGALLVASERAVQTHNLTPLARVHTLAVVGDDPVYM
                     LTGPIPATAAALSRSGLGIDDIDLFEVNEAFAPVVLAWAHETGASLERTNVNGGAIAL
                     GHPLGATGAILATKLVHELHRTGGRYGLQTMCEGGGQANAT"
BASE COUNT          743 a         1857 c         1730 g          677 t
ORIGIN      
        1 gatcgtctgg gtgcgcgcct cactggccag cgcgtcgcgc atcgtgcggt cggcgccctc
       61 gttcagcagc gccttggact gcgcgatcgc caccggcggg ccggcggcca gccgcttgcc
      121 gagatcggtc acgaacgcgt cgatctcctc cgcctccacc acccaggtga ccaggcccag
      181 ccggtgcgcc tccgcggcat cgatcatctc ggcgagcagc gcgagccgtt tctcctgctg
      241 caacccgacc agcttgggca gcaaccacga gccaccgaag tcgagcgaga gcccgcgcct
      301 ggcgaagatc tgcgagaacc gcgcaccggg gctgcagacc acgagatcgc agcccagcgc
      361 caagttccag ccggctccca cggcgacccc gtcgaccttc gcgaccgacg ggaccggcag
      421 ctcgtgcagc aaaagggcca cctcgttgat cccgcgcata cggcccagcg ggtgccccgc
      481 ggcgaccccg cccgagaggt ccgcccccga gcagaagtcg ccacccgccc cggtgatcac
      541 gatcgcccgc accgcggggt cgttgcccgc ggcagtgagg ttctcccgca gcgccgacca
      601 caggtcggcg ttgatcgcgt tcttgcgccc gggccggttc agcgtgaggg tcaggacacc
      661 gccgtcgcgg ctggtgagca ggacgtcggt gcccatccga tctcctccct cagacgccga
      721 gccggtcggc cagccgcgtg cggtgcagcg ccgggccccc gaacagcagc tcagagctct
      781 tggcccgctt gtagtagagg tgcgcgtcgt gctcccaggt gtagccgacc ccgccgtggt
      841 actgcaccat gtccgtcgcc gccgcgaagt aggcgggacc gatataggtc tgggcgagtg
      901 ctgccgccag gggtagctcg gcgcggtcgt ggtcggcgac ccacgccgcg tagcgcaaca
      961 ccgagaccgc gtgctcccat gcgctgtaca tttcggcgca gccgtgcttc acggcctggt
     1021 aggagccgat gagccggccg aactgcacac ggaccttcgc gtagtcgacg gtcagctcca
     1081 tcgcccgctg cagtccaccg acctgctccg cggccagcgc gacggcggcg aggtcggcca
     1141 ccaggtcgaa cgccgccgcc gggtcggcgg cgaccagcgg gcgcgccggg gtggcggcga
     1201 acgtgaggcg ggcgagccgc cgggtcgggt cgaggccggt caggacggtg cgaatgaggc
     1261 cggcagcgtc accgtcgacc gcgaaccagc ccggaccgtc gggtgtgctg gcgtagacga
     1321 gcaccaggtc ggcggtgtcg ccggtgagca cgagcgtctt cgtgccggcg agctcccaac
     1381 tgccgccgcg cgcggtggcg gtggtcgcac caccctggcg atcccacccg gcgccgcctt
     1441 cgccgaccgc gaccgtgccc acgagctctc ccgcggccag ccgcggcagc agctcggtgc
     1501 gggcggcgtc gtcatcgacc gcgagcagga cgtcggtggc gagcacggcg gaagcgagga
     1561 acggcgaggg cgtgagcgcc cggcccagct cctccagcac cacggaccgc tcgacctgcc
     1621 ctgcccctgc gccgccgagc tcctccggca cgaccagccc gagcaccccc agcccggcca
     1681 tccggttcca caggtccgcg tcgtaaccgc ggtccgacgc catcgcctcg cgcaccttcg
     1741 ccgacggcga ccggtcggca aggaacttgc ggacggtgga gcgcagctcc tgctgctccg
     1801 gctcgaggac gagcttcacc ggacaccgcc ctgtcgctgc gtgccgacct tgatttcctt
     1861 gaacgggagg tcacggtcga cctgcggctc cttcggcagg ccgaggatcc gctcaccgat
     1921 gatgttgcgc tgcacctcgt tcgtgccgcc ggcgatgctc gacgccggcg cggagttgat
     1981 gcccagcgcc agctcagccg cgcgcgcatc gtcgggatcc cacgcgacgg cgtcggcgcc
     2041 ggccaggtcg ctcgcggtct cgatcgcgcg gcgcagcagc aacgcgccgg ccagcttggc
     2101 cacggagccg cgcaccccgg gcgggttgcc ggccgcggtc tcctgccgca tccgcgcgtt
     2161 gaacagctcc aacgcgcgct cgtgcgcgta cagctcagcc agccgctcgc gccgggccgg
     2221 gtccgcggcg acgccctcgt gacgggcgag ctcgacgagc gaagcgaact ccagcgggtt
     2281 gctgcgcggc ctgcgggtgg ctccgatcga cacccgctcg tggccgagca ttgccaccgc
     2341 ggcctgccac ccacggttga cctcgccgat caccccgtcg tcggggatcc ggacatcgtc
     2401 gaagtagacc tcgttgaacg gggcgcgccc ggacatgtcc ctcagcggcc gcacggtgac
     2461 gcccggggca cgcatatcga cgatgaacat cgtcaggccc gcgtgcttgg gccggtcggg
     2521 gtcggtgcgg gcgagcaggg ctccgtagtc ggcccactgc gcgttcgtcg tccacacctt
     2581 ctgcccattg accacccagc cgtccccgtc ccgcaccgcg cgcgtctgca ggctcgcgac
     2641 gtccgacccg gcgccgggtt cggagaacag ctggcaccag atctcctcac cgcacaggag
     2701 cctgggcagg taccggtgct tctgctccgg cgtcccgagg tcgaccaggg taggcccgca
     2761 catacccatg ccgatgatga acgggtaggt cggaagctcg aaatcggcgg cctcctcgct
     2821 gaagacctgc tgctccgccg tgcccagccc ctgcccaccg acctccttcg gccaggtgat
     2881 gccggcgtac ccggcgtcgt acagcgcggc ctggaactcc cgggcccggc gcagctgctc
     2941 gtcggacgtg agtccggcgg ccggaccggg ggcatgagcc gtgagccagg cgcgcacgct
     3001 gctgcggtag tcctgggtcg cgtcgaccat gatcatgcgc gacaaccctc cacgacgtcg
     3061 agaggcagat gacccgaact tactgatcac tcgttaagtt gacaacagcc caaccaccca
     3121 ctcaccagac gtcccgccca tatgctgacg ccgtgagcac cgcatccgac ctgccgcccg
     3181 gcgccgtgga caccggtgcc gggccgagcg gcacaccggt cattccccgc ggtggcggcc
     3241 gcgacgccat caccggcgct gcccgcgaga tcttcgccga acgcggctac cacggcgcct
     3301 cgatccgcga catcgccaag cgtgccgggc tcagcctctc cgccatgtac tactggcacc
     3361 ccagcaagca gcacctgctc gcggcgctga tcgaggagag cacctacgac tacttccacc
     3421 ggtgcgacca ggcactgcgc gaggtggggg acgacccggc cgaccggttg cgagcgctgg
     3481 tgcgggccac cgtcgagtac cgcgtgcggc gccgcgtcga gagcaacatc ggcacgcagg
     3541 agtggcgcaa cctggagccc gacaaccggg agcgcctcga cgggctgcgc cgctcggcgt
     3601 cccaactgtg ggccgacatc atcaacgacg gcgtggcgcg cggaacgttc cgatgcgccc
     3661 atcccgacga tgcacgacgt tcgatcgtgg ccgcctgcaa tgcgatcgcg cagtggtacg
     3721 agccgtccgg cgagctcggc ctgcccgatc tcgtcgagcg gtacacggag atcgcgctgc
     3781 ggatcgccga ccaccgcagc tgagctgact ctcccggtca cgttcgattt gactgtacga
     3841 tcgttaagtt accgtcgaat ccgcgacgaa gggggagatt ccggtggctg aggcttacat
     3901 cgtcggtgcc gtccgcacgc ccatcggcac ccgcaagggg gcactggccg gagtccatcc
     3961 agccgacctc ggcgcacacg tcctgcgcga gctcgtcgac cgcaccggga tcgacccctc
     4021 cgcggtcgag gacgtgatca tgggttgcgt ggtgcagctc ggcccgcagg ccggcgacat
     4081 cgcgcgtacc gcgtggctct cggccggcct gccgcagagc gtgccggggg tgacgatcga
     4141 ccggcagtgc ggctcgtccc agcaggcgct gcacttcgcc gctcagggcg tgatgtccgg
     4201 gacacaggat ctcgtcgtcg cggcaggcgt cgagaacatg gcgatggtgc cgatgggcac
     4261 caacgccgcc gtcagcaagg agctcggagc gtcgccgaag ggcgacgggt gggcggagcg
     4321 gtacggcgac caggagatct cccagttccg cggcgcgcag ctgatctgtg agaagtgggg
     4381 ctaccgccgc gacgacctcg agcgcttcgc cctggagagc caccggcggg cggtgcgggc
     4441 gatcgacgag ggacgcttcg ccgggcagat caccccggtc gccgggaccg acaccgacga
     4501 gggcgcgcgc cgcgacacgt cgctggagca gatggcgcag ctcaagggcc tgcgcgaggg
     4561 ctgggagatc accgccgccg tctcgagcca gatgtcggtg ggcgcgggcg cgctgctcgt
     4621 cgcctccgaa cgagcggtgc agacgcacaa cctcaccccg ctggcgcggg tgcacaccct
     4681 cgcggtcgtc ggcgacgacc cggtctacat gctcaccggc ccgattccgg cgacggcggc
     4741 cgccttgagc cggtccggcc tcggcatcga cgacatcgac ctgttcgagg tcaacgaggc
     4801 gttcgccccg gtcgtgctgg cctgggcgca cgagaccgga gcgtcgctgg agaggaccaa
     4861 cgtcaacggc ggtgcgatcg cgctcggcca cccgctcggc gccaccggcg cgatcctggc
     4921 cacgaagctg gtgcacgagc tgcaccgcac cggcggtcgc tacggcctgc agacgatgtg
     4981 tgagggcggc gggcaggcca acgccac
//
LOCUS       DASRSC010000284         1890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4042555,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000284 DASRSC010000000
VERSION     DASRSC010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4042555"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..653)
                     /locus_tag="VFE39_14080"
     CDS             complement(162..653)
                     /locus_tag="VFE39_14080"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET6259772.1"
                     /translation="MRAWLLELFAPDRTPACEIIRSTPGELVGWTTYDLPSLRTWHRD
                     RMIVIGDAAHATSPASGQGASMAVEDAVELARCLRDLPEPTAAFTAYDRLRRARVEKV
                     VAAGARASNSKAAGPVGRVLRDAFLPLVLRTTGRRAQSWLHEYHIDWEAPVGAGGNGM
                     LAR"
     gene            complement(660..851)
                     /locus_tag="VFE39_14085"
     CDS             complement(660..851)
                     /locus_tag="VFE39_14085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259773.1"
                     /translation="MSFVHGHARDTGWVVAHVRRPTRAEDGQLPLLAVVPLPRPAEPD
                     EPEEPESVSSARKSAITRS"
     gene            complement(915..1388)
                     /locus_tag="VFE39_14090"
     CDS             complement(915..1388)
                     /locus_tag="VFE39_14090"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6259774.1"
                     /translation="MSSVRIESVALDEPVVRELIGELDAELQDTYPEEGAVHDRLDAA
                     EVAPGHGVLLVAIEDGTDVPLGCGAVRLREPGIGEIKRMYVRPQARGRGIGRALLSAL
                     EAEAAALFADRVVLETGARQVEAVELYRRAGYAEIERFGEYADAPLSLCMGRELG"
     gene            1442..>1890
                     /locus_tag="VFE39_14095"
     CDS             1442..>1890
                     /locus_tag="VFE39_14095"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6259775.1"
                     /translation="MQDPATAFDAASDTYDDNPHHGTIAEALVRGLRLTIPPALVVDV
                     ATGTGVAAFAALRLLAPERVVAVDIASRMLDRARAKAVHEDPAGRVDWRLARAVPLDL
                     RDGRADVVLCASSLHFLGAAALHDWLRVLRPGGQVAFSIPMADDFHPS"
BASE COUNT          258 a          737 c          639 g          256 t
ORIGIN      
        1 gcctgccgac cggtatgatg atcatcggaa agcgtttcga cgacgccacc gtgctgcgcg
       61 tggcccacac gtacgagcag gcagtcggcg gattccccgc cccgcatgcc gcgtccgtgg
      121 gcagcgcggg ctgacgaagc cgcggcatgc tcgcccgccc cctaccgggc gagcatgccg
      181 ttccctccgg cgccgaccgg cgcctcccag tcgatgtggt actcgtgcaa ccacgactgc
      241 gcccgccgtc ccgtcgtgcg gagcacgagc ggcaggaacg cgtcgcgcag cacgcggccg
      301 actgggccgg cagccttcga gttgctcgcc cgcgcccctg cggccacgac cttctccacc
      361 ctggcccggc gtagccggtc gtacgccgtg aacgcggcag tcggctcggg gaggtcgcgc
      421 aggcagcggg ccagctcgac cgcgtcctcg acggccatcg acgcgccctg ccccgacgcc
      481 ggggacgtgg cgtgtgccgc gtcaccgatg acgatcatgc ggtcgcgatg ccatgtgcga
      541 agcgacggca ggtcgtaggt cgtccacccg accagctccc ccggcgtcga gcggatgatc
      601 tcgcatgccg gcgtccggtc gggcgcgaac agctccagca gccacgcccg caacgccgct
      661 cagctgcggg tgatcgccga cttcctggcc gagctgaccg actccggctc ctccggctcg
      721 tccggctcgg cgggccgtgg gagcggcacc acggcgagca gcggaagctg accgtcctcg
      781 gcgcgggtgg gacgccggac atgagcgacc acccagcccg tgtccctggc gtggccgtgt
      841 acgaacgaca cccccatcac ctccctgccc agtggagcac gaggcaccga caggacgccc
      901 ggccggacca gccgctatcc gagctcccgg cccatgcaca ggctcagcgg agcgtcggcg
      961 tactcaccga agcgctcgat ctcggcatac ccggcacggc ggtagagctc gacggcctcg
     1021 acctgccggg ctccggtctc cagcaccacg cggtcggcga acagtgccgc cgcctcggcc
     1081 tccagcgcac tcaggagcgc cctgccgatt ccccggcccc gggcttgcgg tcgcacgtac
     1141 atccgcttga tctcgccgat gccgggctcc cggaggcgca cggctccgca gccgagcggg
     1201 acgtccgtcc cgtcctcgat cgcgacgagg aggaccccgt gacccggcgc cacctcagct
     1261 gcgtcgaggc ggtcgtggac cgcaccctcc tccgggtacg tgtcctgcag ctcggcgtcc
     1321 agctcgccga tcagctcccg gaccacgggc tcgtcgaggg cgacggactc gatccgtacg
     1381 gacgacatcg gcgcaggata ccgaggcgac ggtgatccgg atgctccgta ggatcccgcc
     1441 ggtgcaggac cccgcgactg ccttcgacgc cgcctccgac acctacgacg acaacccgca
     1501 ccacggcacg atcgccgaag cgctggtccg cgggctgcgc ctgacgatcc cgcctgccct
     1561 ggtcgtcgac gtggccaccg gcaccggcgt cgccgcgttc gccgctctgc ggctgctggc
     1621 gccggagcgg gtggtggccg tcgatatcgc gtcgcggatg ctcgaccggg ccagggcgaa
     1681 ggcggtgcac gaggaccccg cgggccgggt cgactggcgc ctcgcccgag ccgtgccgct
     1741 cgacctccgc gacggcagag ccgacgtcgt gctctgtgca tcgtcgctgc acttcctcgg
     1801 tgcggctgcc ctgcacgact ggctgcgcgt actgcgaccg ggcggccaag tggccttcag
     1861 catcccgatg gccgacgact tccatccgtc
//
LOCUS       DASRSC010000285         5305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2242942,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000285 DASRSC010000000
VERSION     DASRSC010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2242942"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VFE39_14100"
     CDS             complement(<1..270)
                     /locus_tag="VFE39_14100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET6259776.1"
                     /translation="MNTPPTVDSDLGWALGTVMRSYLRAANSAVADVPGGPRGYQVLS
                     AAARGETGSQLALAQLLGVDRTVMTYLIDDLEKAGLVARKPDPADR"
     gene            378..1049
                     /locus_tag="VFE39_14105"
     CDS             378..1049
                     /locus_tag="VFE39_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET6259777.1"
                     /translation="MTLFRLDASIRREGSVSRDVADTVVDAWIRHHPDDTVVRRDLTA
                     DPLPSDAWMFAASATGVPEEERTPEQRSALELAATLADEVLAGDAAVIASPLYNFGVS
                     QHLKTWIDLLITDPRFAPGAQPLAGRPLTVVIARGGGYGEGTPREGWDHGTPYLRRIF
                     ADAFGADVTVVVAELTLADVVPAMAELRPIAAESRAEAHKLAEATGRAHAELVRSATR
                     STSAA"
     gene            1105..2109
                     /locus_tag="VFE39_14110"
     CDS             1105..2109
                     /locus_tag="VFE39_14110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET6259778.1"
                     /translation="MVAFGYSLLCEQRGPRELVADAVAAEDAGFDFEVISDHYFPWLD
                     AQGHAPNAWPVLGAVAAATERVPLMTYVTCPIMRYHPAVVAQKAATVALLAGEGRFTL
                     GLGAGENLNEHVIGRGWPPVNVRHEMLGEAIEIIGELFAGGYVNRKGAHYRVDSAKLW
                     DVPDGGVPIAVAVSGRQSIEAFAPVSDAMVAVEPLPELVQGFDAATGSTLPKIGQQPI
                     SWGADRDEAVARAHEQFRWFAGGWKVNAELPGTAAFAAATQFVRPHDVAAAIPCGPDV
                     AAHVEAVRPFVEAGFTHIALVQVGGETQPDFMAFAAQALLPALREEYGKPQDARIRVR
                     "
     gene            2735..3781
                     /locus_tag="VFE39_14115"
     CDS             2735..3781
                     /locus_tag="VFE39_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET6259779.1"
                     /translation="MAATIRDVATAAGVSQSTVSRALSMPELVNAATRARVLAAARQL
                     GYQPNRAARGLITGRTGNLGLIVPDLSNPFFSSVVKGVQAAARTSDYSLFIADTDEEA
                     SAEVELLRALTQQVDGIVLCSPRSPDAELAEVGVDSAVVLMNRRIDGRPSVTVDNADG
                     MRQAVEHLAALGHERIAYVGGPRTAWSNGQREQGLHRSADATGTDLVHLGHFPPQFEG
                     GVAAADLALASSATAVIAYNDVVALGLLSRLGMRGVPVPEQISVLGCDDIGMSAMTHP
                     ALTTVSIPKREAGRAGVAMLLALLRDGAGAAAGASAPHRELPTQLIVRATTGVAPPTD
                     RRTAGGQSTKGSTR"
     gene            3921..4079
                     /locus_tag="VFE39_14120"
     CDS             3921..4079
                     /locus_tag="VFE39_14120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259780.1"
                     /translation="MAPPESQQVIAQYGRPEYDALIRDHPTATPLATKDIVAAFDRWD
                     REIGGRGQ"
     gene            4076..4224
                     /locus_tag="VFE39_14125"
                     /pseudo
     CDS             4076..4224
                     /locus_tag="VFE39_14125"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein"
     gene            4275..4486
                     /locus_tag="VFE39_14130"
                     /pseudo
     CDS             4275..4486
                     /locus_tag="VFE39_14130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005451969.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            4489..>5305
                     /locus_tag="VFE39_14135"
     CDS             4489..>5305
                     /locus_tag="VFE39_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005450081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HET6259781.1"
                     /translation="MDEPRLATSTLDRITPGVPVATRPPDATVGIVHLGIGAFHRAHQ
                     AVFTEEAMAAEGVGRWGICGVTQRSRAVVDQLGPQDGLYGVLERSDRGVSVRVVGSVL
                     EVVSATEQPELVRSRIADPAVGVVTLTVTEKGYRRDPAGRLDLHDEAVQADVAGAAAP
                     VTAIGRLVRGLQRRVESRAPLTVVSCDNLVANGAVVRALVADFCGALPGAEGEPLWDW
                     VTSSVSFPSSMVDRIVPATTDDDREQARRLLGVRDDGLVVAEPFRQWVIEDDFA"
BASE COUNT          759 a         1903 c         1903 g          740 t
ORIGIN      
        1 gcggtcggcc ggatcgggct tgcgcgccac cagcccggcc ttctcgaggt cgtcgatcag
       61 gtacgtcatc acggtgcggt cgacgccgag gagctgggcc agcgcgagct ggctgcccgt
      121 ttcgccgcgc gccgccgcgg acagcacctg gtacccccgt ggtccgcccg gcacatctgc
      181 gaccgctgag ttcgcggccc gcagatagga ccgcatcacc gtgccgaggg cccaccccag
      241 gtcggagtcg acggtcggcg gtgtgttcac gggcacatcc tacctaggga atccgcagca
      301 caggacgcgc tgttgacacg atcgtctgta gtacagacta tcttggcgcc agtacgacta
      361 cgactgcagg agttccgatg accttgttcc gcctcgatgc cagcatccgt cgcgagggct
      421 cggtgagccg agacgtagcc gacaccgttg tggacgcctg gatccgccac cacccggacg
      481 acacggtcgt ccggcgcgac ctcaccgccg atccgctgcc gtccgacgcg tggatgttcg
      541 ccgcctcggc gaccggcgtc cccgaggagg aacggacgcc ggagcagcga tccgccctcg
      601 agcttgccgc gacgctggcc gacgaggtgc tcgccggtga tgccgccgtg atcgcgagcc
      661 cgctctacaa cttcggcgtg tcgcagcacc tcaagacgtg gatcgacctg ctcatcaccg
      721 atccgcgctt cgcgccgggg gcccaaccgc tcgccggccg ccccctgacc gtcgtgatag
      781 cccgtggcgg cggctacggc gagggcactc cgcgggaggg ctgggaccac ggcacgccat
      841 acctgcggcg gatcttcgcc gacgccttcg gggcggacgt cacggtcgtg gtggccgagc
      901 tgaccctggc cgacgtggtc cccgcgatgg ccgagctgcg cccgatagcc gccgagtccc
      961 gggccgaggc gcacaagctc gccgaggcca ccggccgggc gcacgccgag ctggtgaggt
     1021 cggccacgag gagcacctcg gcagcgtagg tcgccacggc caggggtacc cgccggtcga
     1081 ccgatcaacg atcgagagga cgtcatggtc gccttcggct actccctgtt gtgcgagcag
     1141 cgcgggccgc gcgagctcgt cgccgacgcc gtcgccgccg aggacgcggg cttcgacttc
     1201 gaggtgatca gcgaccacta cttcccctgg ctcgacgcac agggccacgc gcccaacgcc
     1261 tggcccgtgc tgggggccgt cgccgcggcg accgagcgcg tcccgctcat gacctacgtg
     1321 acctgcccga tcatgcgcta ccaccccgcg gtggtggcgc agaaggcagc cacggtggcc
     1381 ttgctggcgg gcgagggacg cttcaccctc ggcctgggcg caggggagaa cctcaacgag
     1441 cacgtgatcg gccgcggctg gcccccggtg aacgtgcggc acgagatgct cggtgaggcc
     1501 atcgagatca tcggggagct gttcgcgggc ggctacgtga accggaaggg cgcgcattac
     1561 cgcgtcgatt cggcaaagct gtgggacgtc ccggacgggg gcgtgccgat cgccgtcgcg
     1621 gtgtccgggc ggcagtcgat cgaggcgttc gcgcccgtgt ccgacgcgat ggtggccgtc
     1681 gagccgcttc ccgaactggt acagggtttc gacgccgcca cgggcagcac cctgcccaag
     1741 atcggacagc agccgatcag ctggggcgcc gaccgcgacg aggcggtcgc gcgggcccac
     1801 gagcagttcc gctggttcgc gggcggctgg aaggtcaacg ccgagctacc agggacagcc
     1861 gccttcgccg ccgccacgca gttcgtgcga cctcacgacg tggcggcggc gattccctgc
     1921 ggccccgacg tggctgcgca cgtcgaggcg gtccgcccgt tcgtcgaggc cgggttcact
     1981 cacatcgcgc tggtgcaggt ggggggcgag acacagccgg acttcatggc gttcgccgcg
     2041 caggccctgc tgcccgcgct gcgcgaggag tacggcaagc cccaggacgc ccggatcagg
     2101 gtgcgctgag ccgcagcacc gggccgggcc gaacgaccga cccgctggtc cggacctgtc
     2161 gcgccaaagc gcgttcgctc gacggggccc gagcactgcg ggccgcgggg cgggcggacg
     2221 tgcgaggggc cccttcgtgc ggacctaccg tgcgcccggg cgtcgtccac gaacccggtg
     2281 gtgttgtaga agcccgcggt gtcggtgccg gcctcgcgga agcggcgcat gccgtccggc
     2341 gggtcaggaa ccaccagggt gcaccgagcc ggatcgccgg gtggacgctc ccccaccccg
     2401 gctcccgcag cacgccgtgg tcgggcaacg tcgacagggg ggagtcggtg gcggccagca
     2461 cctcgatccc gaactgctcg aacagcgccc gcggcccgaa gtcgggcttg gccagcttgt
     2521 cggcgatgtg gtcgtagagc gcgtcgggcc ggtgtcctcg ccgggctggg tggtcaaagt
     2581 ggacgcatgc gaccttcaaa tcccattgca aaggattgca agatcccgca ttgattggca
     2641 agcatccacg gggatcaagg gccttgacgc cagcagcaaa cgatttcaca atctcccaca
     2701 cacgacacga ccgacccaac gatggaggga cgccgtggcc gccacgatcc gcgacgtcgc
     2761 aacggcggca ggtgtctcgc agtccacggt gtcgcgcgcc ttatcgatgc cggagctggt
     2821 caacgcggcc acccgcgcgc gggtgctggc ggcggcccgc cagctcggct accagcccaa
     2881 ccgcgcggcc cggggcctga tcacgggccg caccggcaac ctcggactca tcgtgcccga
     2941 cctgtccaac cccttcttct cctccgtggt gaagggcgtg caggcggcgg cccggacgag
     3001 cgactactcg ttgttcatcg ccgacacgga cgaggaggcg agcgccgagg tcgagctgct
     3061 gcgggcgctc acccagcagg tcgacggcat cgtcctgtgc tcgccccgct caccggacgc
     3121 cgagctcgcc gaggtcggcg tcgacagcgc cgtcgtcctc atgaaccggc ggatcgacgg
     3181 gcgcccgagc gtcaccgtcg acaacgccga cgggatgcgg caggcggtcg agcacctggc
     3241 cgcgctcggc cacgagcgga tcgcgtatgt cggcgggcct cgcacggcct ggtcgaacgg
     3301 ccagcgggag cagggtctgc accggagtgc ggacgcgacc ggcaccgacc tcgtgcacct
     3361 ggggcacttc ccgccccagt tcgagggcgg cgtcgcggct gcggacctgg cgctggcctc
     3421 cagcgcgaca gcggtgatcg cctacaacga cgtggtcgcg ctgggactgc tgagccgcct
     3481 cgggatgcgg ggggtgccgg tgcccgagca gatcagcgtc ctgggctgcg acgacatcgg
     3541 gatgtcggcg atgacccacc ccgcgctcac caccgtctcc atccccaagc gggaggccgg
     3601 tcgggccggt gtggcgatgc tgctcgcact gctccgggac ggtgccggcg cggcggccgg
     3661 cgcctccgcg cctcaccgcg agctccccac ccagctcatc gtgcgcgcca ccaccggcgt
     3721 cgcgcccccc accgaccgcc ggacagcggg cggtcaatcc acgaagggat cgacccgatg
     3781 acgttgcgct ggcgcaccgc tcgaaccact ggtggggtct ccgcgttact gctcatggcc
     3841 ggctgcggcg ctccgagcgg gaggcctacg acacggggta cttctacccc ggccccgcgg
     3901 tcgagggcgt cacgctccag atggctccgc cggagagcca gcaggtgatc gcgcagtacg
     3961 gccgccccga gtacgacgcg ctgatccgcg accaccccac cgccaccccg ctggccacga
     4021 aggacatcgt cgcggccttc gaccgctggg accgcgagat cggcggtagg ggccaatgaa
     4081 tggaccatcg gcacacggcg cgttcaccga actgcggctc gacggcgtcg ggcgccggtt
     4141 cgccggcaag gacgcgctgg tcaacctcga cctgacgatt caggccggcg agttcatcgc
     4201 tctgctcggg ccgtccggct gcggcaagtc cacggcgctg aactgcctgg ccggactgct
     4261 ccccctgacc tcgggagctc acgttcctca ccgccgggcc ggactcgcag accctcgtgg
     4321 tcactctgta cgactcgatg tcggccgccg ggatccgggc gcagcagtcc gtcgacgcga
     4381 tggccgtgat ctacacctcg acgatgctgg tgctgcttgt cgtcgcgctg cggttcgtaa
     4441 ccccacgcag ctggtagcca gagtcaagga gacgcccgaa ccgtgaacat ggacgagccc
     4501 cgcctcgcaa cctcgacgct cgacaggatc acccccggcg ttccggtggc cacccgcccc
     4561 ccggacgcca cggtcggcat cgtccacctc ggcatcggcg cgttccaccg ggcacaccag
     4621 gcggtgttca ccgaggaggc gatggcggcc gagggcgtcg gacgctgggg gatctgcggc
     4681 gtgacgcagc gctcgcgagc cgtcgtcgac cagctgggcc cgcaggacgg cctgtacggc
     4741 gtgctcgagc gcagtgaccg tggtgtctcc gtccgcgtgg tcggttccgt gctcgaggtg
     4801 gtgtcggcga cggagcagcc cgagctggtg cggtcgcgga tcgcggaccc ggcggtgggt
     4861 gtggtgacgc tgaccgtcac cgagaagggc taccggcgcg atcccgccgg caggctcgac
     4921 ctgcacgacg aggcagtgca ggcggacgtg gcgggcgccg ccgcgccggt gacggcgatc
     4981 ggccggttgg tgcgcggtct ccagcgcaga gtggagagcc gcgccccgct caccgtggtc
     5041 tcctgcgaca acctcgtcgc caacggcgcg gtcgtccgcg cgctggtggc ggacttctgc
     5101 ggcgccctgc ccggcgcgga gggcgagccg ttgtgggact gggtgacgtc gtccgtctcg
     5161 ttcccgagca gcatggtgga ccggatcgtg ccggccacca cggacgacga ccgggagcag
     5221 gcccgccgcc tgctgggcgt gcgggatgac gggctggtgg tggccgagcc gtttcgccag
     5281 tgggtgatcg aggacgactt cgccg
//
LOCUS       DASRSC010000286        17235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5840994,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000286 DASRSC010000000
VERSION     DASRSC010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 17235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17235
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5840994"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..663
                     /locus_tag="VFE39_14140"
     CDS             64..663
                     /locus_tag="VFE39_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259782.1"
                     /translation="MTDPELEGPTSVFRLDPELIDAVVGGAAGRQADAALAEQVSTVL
                     PWHATLPIDIAAQLARLARHLGGDDALLRWLDEHPGRPRVVTRAFRLVGLLDQISGSA
                     AVVTALRELRERTPYPPGLEGYLVPDTHSGTLASLAQQIEGLLGDDRPDDAVALSLAT
                     IDALHDVLPRAVELDPDLRGLEEQLGQLRCEIEEAREPA"
     gene            783..2108
                     /locus_tag="VFE39_14145"
     CDS             783..2108
                     /locus_tag="VFE39_14145"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6259783.1"
                     /translation="MDSLRPAAETAGRRPRVVVIGTGFAGFHCLRTLERRLPPGAADV
                     LAVNPADYMLYVPLLPEVAAAILEPRHVAVPLRARLPRTRVVLGNTADIDVAARTCSV
                     LDIERRRRTLQWDRLVVACGSVTRLLSVPGVADHANGFKSIAEAVYLRDHILRQLELA
                     EQASDPAERAARTTFVVVGAGYTGTEVVGQGQLLTRDALRQFPGLRPDEVRWLLLDLA
                     PRVLPGLNPRLSAPAERVLRRRGVDVRLRTTVSEVTPTCAVLSDGTEIPTRTVVWCVG
                     VRPDPLVETLGLPTRAGRLVVDECLTVPGHDDIYAAGDVAAVPDPGRPGEITAMTAQH
                     AQRQGELAGINIAASLGHGQARPYRHADKGFVVDLGGSQAVADPFHVPVTGVVAKAIT
                     RGYHLAALPAGRSRVALDWLVDSLAGRPLVQFGFVSETAVRLADVDRIV"
     gene            2142..4151
                     /locus_tag="VFE39_14150"
     CDS             2142..4151
                     /locus_tag="VFE39_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HET6259784.1"
                     /translation="MTDRLFRDRRDAGRTLAGLLEHYRGRDDVVVLGLPRGGVPVAYE
                     VAQALGAPLDVFVVRKLGVPGREELAMGAIASGGVVVINDDVVRGLGITSDVVQRVAE
                     QEGRELHRREQEYRGGRPMPELEGKTVVLVDDGLATGSSMRAAIAALRRHRPSRIVVA
                     VPAAPGSTCQELSTYVDEVVCATTPSPFYAVGASYWNFDQTTDEEVRALLRAAATSTP
                     AYAAERVLSDADVIRSSVIATEEGVPPDEALFELVGDARLVLIGEASHGTHDFYAARA
                     TMTRRLIEEKGFCAVAVEADWPDAYRVNRYVRGRSDDADAEEALRGFQRFPTWMWRNA
                     DVLDFVGWLRERNDRIDDERRAAGFYGLDLYSLHRSIDEVITYLDRVDPAAARRARER
                     YACFDHYGTDDGQTYGMAAAMGAGETCEREVLEQLQDLQRHGVEYARRDGLLAEDEQF
                     YAEQNARTVKNGAEYYRSMFSGRVLSWNLRDQHMVETLDAVQNHLSRRSGSPAKIVVW
                     EHNSHLGDARATESAARGELNVGQLVRERYGRDSRNIGFTTYTGTVTAADDWGGEAKR
                     KWVRPALPGSVEELFHDVGQKEFMLSFATATRAAETLRTARLERAIGVIYRPETERQS
                     HYFRARPADQFDAVIHIDDTRALEPLERTGVWETGEVPETYPFAV"
     gene            4379..5062
                     /locus_tag="VFE39_14155"
     CDS             4379..5062
                     /locus_tag="VFE39_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HET6259785.1"
                     /translation="MSAPDITRQSLRIPVAGVVLDADVGLPESPRGAVLFAHGSGSGR
                     RSPRNRYVADVLGRAGVVTVLADLLTAEEERIDMQTGTLRFDIGLLAERVTTLTDWVA
                     QNESFAGLGIGLFGASTGAAAALIAAAGRPAVVQAVVSRGGRPDLAGPRLHEVRQPTL
                     LIVGGRDALVLELTREAMRKLGGTVRLAVVPDATHLFEEPGALEQAARLARDWFLQHL
                     RPVPRDGDD"
     gene            5095..5454
                     /locus_tag="VFE39_14160"
     CDS             5095..5454
                     /locus_tag="VFE39_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259786.1"
                     /translation="MTVKRNERAYDHARELIAAGRYVIDDRDAWSEHRPSAQQENDFI
                     EQHGIGEYARWHLGIDDQEDENRKSRYKFPYGDFDKVHRCGLLAAESRAGQRKYFDIE
                     LAVAHLHGMLEALRRTG"
     gene            complement(5510..6751)
                     /locus_tag="VFE39_14165"
     CDS             complement(5510..6751)
                     /locus_tag="VFE39_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET6259787.1"
                     /translation="MIEVAQRAALGATSQSRPLEGLHVVECASFVAGPTGGMTLAQLG
                     ADVIRIDPVGGGSDHRRWPVAADGESYYWASLNKGKRSVAVDMRSDEGREIVIALITA
                     PGLDRGVLIDNVVGRRWMSHDALAARRPDLIHLRVQGYPDGRPAVDYTVNAEVGIPLI
                     TGTEEGGAPVNHVLPAWDLVTGLSVATGVLAALHDRARTGNGAFVELALSDVALAGVA
                     NLGWLSETAASGRDRPRHGNHVYGSFGVDFACSDGQRVMVVALTEGQWYALRTVTGTG
                     PVFDALEASLDADLTRESERYRLRETIAAILRPWFAGRDLAQVSDELDAARVLWGRYL
                     PMTGLLEAHRTGAHPVLADIDMPGGGSAITARSPLRWNGKHGAPGEAPLLGRDTEQVL
                     SEVLGLQQAELGALHDRGVIA"
     gene            7084..7629
                     /locus_tag="VFE39_14170"
     CDS             7084..7629
                     /locus_tag="VFE39_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HET6259788.1"
                     /translation="MTEQPRYSDASAGSGRPVAGGPWSPGSPAAPHRSARRTIASFGR
                     YEDAERAVDRLSDLGFPVERVAIIGQDLQTVEQVTGKMDYPRAAWRGALSGAVPGALI
                     GWIFGLFNWVNPLITAVLLALYGAIIGAVIGAIIGVIIYALQRGRRDFASVTVMRPQG
                     YEVVVDDEVADEAARLLGTGV"
     gene            7631..9520
                     /gene="dnaK"
                     /locus_tag="VFE39_14175"
     CDS             7631..9520
                     /gene="dnaK"
                     /locus_tag="VFE39_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET6259789.1"
                     /translation="MARAAGIDLGTTNSVIAAWEAGQSQVVPNAEGARTTPSVVAFLE
                     NGERLVGQLARRQAILNPKGTIYSVKRFIGRRFEEVEEEAKQVTYDVVPDENGLARIK
                     VRDKLYAPEEISALILRKLADDAGRHLGERVTEAVVTVPAYFNDAQRTATRDAGKIAG
                     LEVLRIINEPTAAALAYGLDKRQHETVLVFDLGGGTFDVSILDVGDGVVEVRATAGDT
                     HLGGDDFDRRIVDHLAEQFQKENSIDLRKDPQALQRLYEAAEKAKVELSSVTQTQVNL
                     PFITADANGPKHLTMTLMRSTFEELTRDLVDRTMEPVRRAMSDAKVGPDDIDEVILVG
                     GSTRIPAVQALVRRLTGGKEPNMTVNPDEVVAVGAGIQAGVLKGDVSDVLLLDVTPLS
                     LGVETQGGVMTRVVDRNTTIPVRRSETFSTAADNQPAVDVVVLQGERERAADNRVLGR
                     FQLTDIRPAPRGEPQIEVVYDIDANGILNVTARDKDTAAEQGITISESTNLDSSEVDR
                     MVQEAERNRSSDQQLRQEVDARNELDSLAYQVQRRLDELGDAAPSHERARAEMLVADA
                     RRAVEEQAPLDRVRQLTGELQQVLAGLSAVSASAGAGGAQGGGSEGATSETEDDVIDA
                     EFDRS"
     gene            9530..10111
                     /locus_tag="VFE39_14180"
     CDS             9530..10111
                     /locus_tag="VFE39_14180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET6259790.1"
                     /translation="MTEQAQDRTQSQPAGVVADQGGAAPEERGGAGSGTPAEGSAEDR
                     LDSGPDAAELEDRWRRALADLDNLRKRYARELGREREAERELVASAFLPVLDTIDRAL
                     EHAGADPRSIVEGVRTLREQALAVLAGLGYRREDETGVPFDPARHEVVGVVEADGGDA
                     APGSVASVVRPGYGAPGRQLRPAAVTVAQTPGG"
     gene            10115..11155
                     /locus_tag="VFE39_14185"
     CDS             10115..11155
                     /locus_tag="VFE39_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HET6259791.1"
                     /translation="MARDYYETLGVSRNAGPDELQQAYRRLARENHPDVNRDPGAEER
                     FKEINDAYHVLSDPSLRKRYDRFGDDFRQVPEDWEERVGAGAGQFRGAPGGGAGGGGG
                     RRYRWSPGGDGGPDFGRDYGGGDFGGRDFGGGFGGVDIEDLLGGMFGGRGRGRAGPVV
                     GADQEAELSLTVEEAYRGGRRQVTLNGPDGPRTYTVTIPRGVTDGTRIRLAGEGGGGM
                     GGAPAGDLYLVVRLLPHPRYRVQGKDIVVDLRLAPWEGALGATVPVTTPGGETKVTVP
                     PGTSTGRRLRLRGEGMPDPRGKPGDLYAEVKIMVPPRLTDEERRLFSELAAVSTFDPR
                     RSGSDEGRGARR"
     gene            11152..11544
                     /locus_tag="VFE39_14190"
     CDS             11152..11544
                     /locus_tag="VFE39_14190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HET6259792.1"
                     /translation="MTAQYLPIRYRPTRGSGRGWLGQEEFARRCGLHPELVRRFVALG
                     LVPAVRDADGALWFSTSQVAAVARLQRLRAALPLNYAALGLVVDLLDRITELETALRV
                     RGHTEPVRPPARPQQPTAIDQKRSERWT"
     gene            11535..14159
                     /gene="clpB"
                     /locus_tag="VFE39_14195"
     CDS             11535..14159
                     /gene="clpB"
                     /locus_tag="VFE39_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HET6259793.1"
                     /translation="MDLNRLTQMSQQALSAAQSVATRAGHTEVDGEHLLLALLDQTDG
                     LVPRLLVAMGVDEGALLSDLEAELARKPRTTRPAADPGQVSITQRLARALEAAEREAR
                     RMKDEYVSVEHLLVALAEEGSATGAGRLLAKHGVTRDGFLAALTRVRGNQRVTSATPE
                     GTYEALVKYGIDLVESARSGRLDPVIGRDAEIRRVVQILSRKTKNNPVLIGDPGVGKT
                     AIVEGLAQRIVNGDVPEGLRDRTIFALDMGLLVAGAKYRGEFEERLQAVLAEVKGAEG
                     RILLFIDELHNVVGAGAAEGAMDAGNMLKPMLARGELHMIGATTVQEYRKRIESDAAL
                     ERRFQPVYVDEPTVEDTVSILRGLRERLEVFHGVRILDGALIAAATLSHRYLTDRFLP
                     DKAIDLVDEACARLRTEIDSMPAALDEITRKVMRLEIEEAALAKEEDPASVARLDQLR
                     RELADLRSEADAMRAQWDAERQAIKRVQELRAELEQVRREIEEAERSYDLNRAAELRY
                     GTLAELERRLEAEGQRLAEKQGEHRLLREVVTAEEIAEIVSVWTGIPVSRLTESEREK
                     LLRLDEVLHERVVGQDEAVQAVADAIIRARSGVKDPRRPTGSFIFLGPTGVGKTELAK
                     ALAEALFDSEDNMVRIDMSEYQERHTVSRLVGAPPGYVGYEEGGQLTEAVRRRPYAVV
                     LFDEIEKAHADVFNTLLQVLDDGRLTDAQGRTVNFRNTVIIMTSNIGSQYMLDGVTPD
                     GQLKPDARELVLAELRSHFRPEFLNRVDDTVLFTPLTLSEIEHIVELMLAELRSRLAD
                     RNLNLEISEDARLFIAREGYDPVYGARPLRRFIAREVETRIARALLREGPADGSTLRV
                     EVEGDQIVVTHHPVGAQV"
     gene            14156..14620
                     /gene="trxA"
                     /locus_tag="VFE39_14200"
     CDS             14156..14620
                     /gene="trxA"
                     /locus_tag="VFE39_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET6259794.1"
                     /translation="MSAPAKPRTSVVTCPHCGQRNRVPAAAASVPRCGNCHNALPWIA
                     DAGDDDFSEVAERSPIPVVVDLWATWCGPCRMVSPALEQLAIERAGDIKLVKVEIDAA
                     PRLAQRFEVRAVPTLLVMRDSEVIARQPGAAPVAALRRWLDQALQPKEGETR"
     gene            14685..14957
                     /locus_tag="VFE39_14205"
     CDS             14685..14957
                     /locus_tag="VFE39_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HET6259795.1"
                     /translation="MTIPVRPDPHLAFARDDVVPRTPQGCEDCLRLGSPWVHLRLCLT
                     CGHVGCCDSSPNRHARAHAGLVGHPIVRSFEPGEDWRWCFADEAFA"
     gene            15027..15878
                     /locus_tag="VFE39_14210"
     CDS             15027..15878
                     /locus_tag="VFE39_14210"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET6259796.1"
                     /translation="MDELGPAGPALRPVGVRESSVAAARHARLPEAATRYTVAVPVHG
                     AVEVSHRAHTVEADAARAAVLQPEGDSDMSFGDGFSCYLVTIDTGKLKDVLEHRLGHA
                     VRRPPDLSESLDLRTPAGRAWARLAGLLASSPVLRHPLLADAVQEMLLARLLLALDHP
                     YRDELDAPVHSWGPGPAHRMVDAIEASPRHPFTVAELSDVAGVSVPALRECCRRHLDT
                     SPTQQLRSIRLTRARRELTEADFGWTSVAGVASGWGFTNTGRFVADFGNRYGEPPWQT
                     LRGPAYA"
     gene            15907..17040
                     /locus_tag="VFE39_14215"
     CDS             15907..17040
                     /locus_tag="VFE39_14215"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET6259797.1"
                     /translation="MSPAPTGGLVTVFVCGDVMLGRGVDQILPHPDAPALRERGVRDA
                     RTYVELAEAVNGLVPRSVGFSWPWGDALALLDELAPDVRVVNLETSITRRGRFAPGKG
                     VHYRMNPANVPCLTAARPDACVLANNHVMDFGQRGLKDTLETLAGAGLRAVGAGRDEA
                     GAWCPAVVSLPGGGRLVIHAFGTPSSGIPVSWAASGQRPGVGFVPELSDAAADRVLDR
                     VQQHGGQLVIASIHWGSNWGYRVFPDQVRFAHRLVDGGVDVVHGHSSHHPRPIEVYRD
                     KLILYGCGDFIDDYEGISGHEGYRDDLRLGYFASIERGTGSMAGLRMVPLQARRLRLC
                     HASGNDAAWLRTVLGRISQDFGTRIDLGPDGMLLLRCTGTDRF"
BASE COUNT         2559 a         5904 c         6350 g         2422 t
ORIGIN      
        1 gttcgcggtg gtctacggcg cggcactcgg tgccctcgcc gtgtcgctcc gctcgccggt
       61 gccgtgacgg atcccgagct ggagggcccg acctccgtct tccggctgga ccccgagctg
      121 atcgacgcag tcgtcggcgg cgcggccggc cggcaggccg acgcggccct cgccgagcag
      181 gtgtccaccg tgctgccgtg gcatgccacg ctgccgatcg acatcgccgc acagctggcc
      241 cggctggccc ggcacctcgg aggcgacgac gccttgctcc ggtggctcga cgagcacccg
      301 ggtcggccgc gggtcgtcac gcgcgccttc cgcctggtcg ggctgctcga ccagatcagc
      361 ggctcggccg cggtggtcac cgccttgcgc gagctgcgcg agcgcacgcc ctacccgccg
      421 ggcctcgagg gttacctcgt gcccgacacc cactcgggaa cgctggcctc cctcgcccag
      481 cagatcgagg gcctgctcgg tgacgaccgc cctgacgacg ccgtggcgct ctcactcgcg
      541 acgatcgacg cgctgcatga cgtgctgccg cgtgcggtgg agctcgaccc ggacctgcgg
      601 gggctggagg agcagctggg gcagctccgg tgcgagatcg aggaggcccg ggagcctgct
      661 tgaggggccg gggtcgcagc cggggcagcg gagcacctgc gtccgacagt gcagggtgga
      721 cggacaccac tgagcctacg aggccgagga ccgcccggtg gtattcgacg accctggccc
      781 gggtggactc actacggccg gcggccgaga ccgcgggaag gcgaccacgg gtcgtggtca
      841 tcggcacggg gttcgcgggt ttccactgcc tgcggacgtt ggagaggcga ctgccgccgg
      901 gggcggcgga tgtcctcgcg gtgaaccccg cggactacat gctctacgtt ccgctgctgc
      961 ccgaagtcgc ggccgcgatc ctggagccgc gccatgtcgc cgtaccgctt cgggcgcggc
     1021 taccgcgaac gcgggtcgtg ctcggcaata ccgccgacat cgacgttgcg gcgcggacct
     1081 gctccgtcct cgacatcgag cggcgccgcc ggacgcttca gtgggaccgg ttggtggtcg
     1141 cgtgtggctc ggtcacccgg ctgctgtcgg tcccaggtgt ggccgatcat gcgaatggct
     1201 tcaaatcgat cgccgaggcc gtctacctgc gcgatcacat cctgcgccag ctggagctgg
     1261 ccgagcaggc gagcgatccg gccgagcggg cggcccggac caccttcgtc gtggtgggcg
     1321 ccggatacac ggggaccgag gtcgtcgggc agggccaact gctcacgcgc gacgcgctgc
     1381 gccagttccc ggggctgcgg cccgacgagg tgcggtggct gctgctcgat ctcgctccgc
     1441 gcgtgctgcc ggggctcaac ccgcgcctgt ccgcgccggc ggagcgggtt ctccggcgcc
     1501 ggggcgtgga cgtgcggctg cggaccacgg tcagcgaggt cacccccacc tgcgccgtgc
     1561 tcagtgacgg cacggagatc ccgacccgca cggtcgtctg gtgtgtgggc gtgcgccccg
     1621 atccgctcgt ggagaccctg ggcctgccca cccgtgcagg ccgcctcgtc gtcgacgagt
     1681 gcctcacggt gcccgggcac gacgacatct acgccgcggg cgacgtggcc gccgtgcccg
     1741 atccgggccg gccaggggag atcaccgcca tgaccgccca acatgcccag cggcagggcg
     1801 agctggccgg gatcaacatc gccgcatcgc tgggacatgg gcaggcccgc ccctaccggc
     1861 acgcggacaa gggattcgtg gtcgacctcg gcggatccca ggccgtcgcc gacccgttcc
     1921 atgtgcccgt caccggtgtc gtcgccaagg ccatcacccg cggctaccac ctggcggcgc
     1981 tgccggccgg gcggtcgcgg gtcgccctgg actggctcgt cgattcgctg gccgggcgcc
     2041 cactcgtgca gttcgggttc gtctccgaga ccgccgtgcg gctcgccgac gtcgatcgca
     2101 tcgtctgacg ccgagcgcgg cgacaacctt caggaggaag catgacggat cggctgttcc
     2161 gtgatcgacg ggatgccggg cggaccttgg cgggtctgct cgagcactac cggggtcgcg
     2221 atgatgtcgt ggtcctgggg ctgccgcggg gtggggtgcc ggtggcctac gaggtcgctc
     2281 aggcactggg agctcccctg gacgtcttcg tcgtccgcaa gctcggggtc cccggacgcg
     2341 aggagctggc gatgggcgcc atcgccagcg gcggggtggt ggtgatcaac gatgacgtcg
     2401 tccgtggcct gggtatcacc tcggacgtgg tccagcgggt ggccgagcag gagggccgcg
     2461 agctccatcg gagggagcag gagtaccggg gcggccggcc gatgcccgag ctcgagggca
     2521 agaccgtcgt tctcgtcgac gacggtctcg cgaccggatc cagcatgcga gccgcgatcg
     2581 cggcgctgcg ccgccaccgc ccttcccgga tcgtcgtggc cgtacctgca gcgccggggt
     2641 cgacgtgcca ggagctctcg acctacgtcg acgaggtcgt ctgcgcaacg acgccgtctc
     2701 cgttctatgc ggtaggcgcc tcctactgga acttcgacca gaccacggac gaggaagtcc
     2761 gtgccctgct gcgggccgcg gcgacgtcga ccccggcata cgccgcggag cgggtcctga
     2821 gcgacgccga cgtgatccgg tcctccgtca tcgcgaccga ggagggcgtg ccgcccgacg
     2881 aggcgttgtt cgagcttgtc ggcgacgcgc gcctcgtatt gatcggcgag gcgtcgcacg
     2941 gcacccacga tttctacgcc gcgcgcgcga ccatgacccg ccggctcatc gaggagaagg
     3001 gtttctgcgc cgtcgccgtc gaggcggact ggcccgacgc ctaccgggtg aaccggtacg
     3061 tccgcggccg aagcgacgac gccgacgccg aggaggcgct acgcggcttc cagcgtttcc
     3121 ccacctggat gtggcgcaat gcggacgtcc tcgatttcgt cggctggctg cgagagcgca
     3181 acgaccgcat cgacgacgaa cgccgcgcgg ccggcttcta cgggctcgac ctctacagcc
     3241 tccaccgctc gatcgacgag gtcatcacct acctcgaccg cgtcgatccg gcggcggcga
     3301 ggcgggcacg ggagcggtac gcctgcttcg accactacgg cacggacgac gggcagacct
     3361 acggcatggc ggccgccatg ggggcaggtg agacctgtga acgcgaggtg ctcgagcagc
     3421 tccaggacct gcaacgccac ggggtggagt acgcccgccg cgacgggctt ctcgcggagg
     3481 acgagcagtt ctatgcagag cagaacgcgc ggaccgtcaa gaacggcgcg gagtactacc
     3541 gctccatgtt cagcggacgc gtcctgtcgt ggaacctgcg tgaccagcac atggtcgaga
     3601 ccctcgacgc cgtgcagaac cacctgtccc gccggagcgg cagcccggcg aagatcgtgg
     3661 tgtgggagca caactcccac ctcggagacg ctcgggcgac cgagtcggcc gcacgcggcg
     3721 agctgaacgt ggggcagctc gtgcgggagc gctacggccg cgactcccgc aacatcggtt
     3781 tcaccaccta caccggcacg gtgacggcag ccgatgactg gggcggcgag gccaagcgca
     3841 agtgggtccg cccggcattg ccgggcagcg tcgaagagct gttccacgac gtcggacaga
     3901 aggagttcat gctgtcgttc gcgacggcca cccgagcagc ggagacgctc cgcactgccc
     3961 gcctggagcg ggcgatcggc gtcatctacc gccccgagac ggagcggcag agccactatt
     4021 tccgcgccag gcccgccgac cagttcgacg ccgtgatcca catcgacgac acgcgggcgc
     4081 tcgaacccct cgaacgcaca ggcgtgtggg agaccggcga ggtgcccgag acctacccct
     4141 tcgcggtctg atgccggttg ccgcggagcg cgtttgcgtc cggcagcgca gggaaggcca
     4201 acgccattgg tcccgggagg ctcaggaccg gccgagtgag acgccgcgaa gctcgcacgt
     4261 ggacatggga cgggctgcgg gccatggtgt gtccacagcc acgaggagac gagcgtgggt
     4321 agcgatcgga atacagctac tggtgggagc gcgtgcgccc tcgctcttgg agtgcgcgat
     4381 gagcgcccct gacatcaccc gtcagagtct gcggatcccg gtcgcgggcg tcgtactcga
     4441 cgccgacgtc ggtctgcccg agtcaccgcg tggggcggtg ctgttcgcgc acggcagcgg
     4501 cagtggccgg cgcagccctc gcaatcgcta tgtcgctgat gtgctcggcc gcgcgggcgt
     4561 ggtcaccgtg ctggccgacc tgctcaccgc cgaggaggag cggatcgaca tgcagaccgg
     4621 gacactgcgg ttcgacatcg gcctgctcgc cgagcgggtc accacgctga cggactgggt
     4681 ggcccagaac gagtccttcg ccggcctggg aatcggcctg ttcggggcca gcacgggggc
     4741 cgcggcggca ctcatcgccg ccgccggccg accggccgtc gtgcaggccg tcgtctcccg
     4801 cggcggccga cccgacctcg ccggaccgcg tctgcacgag gtccgccagc ccaccctgct
     4861 gatcgtcggt ggacgtgacg cactggttct cgagctcact cgggaagcga tgcggaagtt
     4921 gggcggcacg gtccggctcg cggtcgttcc ggacgccacg cacctgttcg aggaacccgg
     4981 cgccctcgaa caggcggctc ggctggcacg ggactggttc ctccagcacc tgcggccggt
     5041 cccacgcgat ggggacgact gacgcgccat cgccaccaca agggatggag accgatgacg
     5101 gtcaaacgga acgagagggc ctacgaccac gcacgagagc tgatcgcggc gggcagatat
     5161 gtcatcgacg accgtgacgc ctggagcgag caccggccgt cggcgcagca ggagaacgac
     5221 ttcatcgaac aacacgggat cggcgagtac gcccgatggc atctcgggat cgacgaccag
     5281 gaggacgaga acaggaagag ccgctacaag ttcccgtacg gcgacttcga caaggtccac
     5341 cgctgcgggc tgttggccgc ggagtcccgc gccgggcagc gcaagtactt cgacatcgaa
     5401 ctcgcggtgg cccacctgca cggcatgctg gaggcattgc ggaggaccgg gtaaccgacc
     5461 gcgacgagca cggactcggc gtcgctggtg ctttcctact ggtgcgttcc taggcgatga
     5521 ccccgcgatc gtggagcgcg ccgagctcgg cctgctggag cccgaggacc tcggacagga
     5581 cctgttcggt gtcgcggccc agcagcgggg cctcgccggg agcgccgtgc ttgccgttcc
     5641 accgcagggg ggagcgcgcg gtgatcgccg acccgccccc aggcatgtcg atgtcggcga
     5701 gaacggggtg ggcgccggtg cgatgggcct cgaggaggcc ggtcatgggc aggtagcggc
     5761 cccacagcac gcgggcggcg tcgagctcgt cgctgacctg ggcgaggtcc cgcccggcga
     5821 accacggccg caggatcgcc gcgatcgtct cccggaggcg gtagcgctcc gactcccggg
     5881 tgaggtcggc gtccaggctc gcctccagag catcgaacac cgggccggtg ccggtcaccg
     5941 tccgcagcgc gtaccactgc ccctcggtga gcgcgaccac catcacccgc tgcccgtcgc
     6001 tgcaggcgaa gtcgacgccg aagctgccgt agacatggtt gccgtgccgg ggccggtccc
     6061 ggccggaggc cgctgtctcg gacagccagc cgaggttcgc gacgccggcc agcgcgacgt
     6121 cggacagggc cagctcgacg aaggcaccgt tcccggtccg tgcgcggtcg tgcagcgccg
     6181 cgagcacgcc ggtggcgacg ctgaggccag tgacgaggtc ccacgccggc agaacgtggt
     6241 tgaccggcgc cccgccctcc tcggtcccgg tgatcagcgg gattccgacc tcggcattga
     6301 ccgtgtagtc gaccgcgggg cggccgtccg ggtagccctg cacccgcagg tggatcagat
     6361 ccgggcggcg cgccgccagg gcgtcgtggg acatccaccg gcgccccacc acgttgtcga
     6421 tcagtacgcc gcggtcgagg cccggcgcgg tgatcagggc gatgacgatc tcacggccct
     6481 cgtcggagcg catatcgacc gccaccgatc gcttgccctt gttcagcgac gcccagtagt
     6541 agctctcccc atcggccgcc accggccagc gtcggtggtc gctgccgccg ccgaccgggt
     6601 cgatccgaat gacgtcggcg cccagctgcg ccagcgtcat cccgcccgtc gggccggcga
     6661 cgaagctggc gcactcgacg acgtgcagcc cttccagcgg cctcgactgt gaggtcgccc
     6721 cgagtgctgc ccgctgtgcc acctcgatca ctctgccccg gtacacccga aggtgcacct
     6781 cgtactcgcc ggcccgcagc ccgattgtgc cgtcccacga cccgagcgcc ccggcttagg
     6841 ccgtgtctcc aggacatcgc aggtccccgc gcgtcgatgg acctggtggg ctgaacacac
     6901 cggacgaggt tgcatcccgc ggaccgatgc gccgtgcctg cggcgctgcg acggtcggct
     6961 gagctcggtt gcccgatgca gcaggagaaa ccccgtgact ctcatgcgtt tcgattcttt
     7021 ccgcgaactc gaccgactgc gtgaacaaac cctgtccgcc gaccgtccga tcggggaggc
     7081 gtcatgaccg aacagccgcg atactccgac gcatctgcag gatcaggtag gcccgtcgcc
     7141 ggcgggccgt ggtcgccagg gagcccggcc gctccgcacc ggtccgcccg gcggacgatc
     7201 gcctccttcg ggaggtacga ggacgccgaa cgcgccgtcg atcgactctc cgacctgggc
     7261 tttccggttg agcgggtggc gatcatcggg caggacctgc agacggtcga gcaggtcacc
     7321 gggaagatgg actatccccg agccgcatgg cgcggcgcgc tgagcggggc tgttcccggc
     7381 gcactgatcg gctggatctt cgggctcttc aactgggtca acccactgat cacggcggtg
     7441 ttgctcgctc tctacggggc gatcatcggt gcggtgatcg gggcgatcat cggcgtgatc
     7501 atctatgcgc tgcagagggg ccgtcgcgac ttcgcgtcgg tgacggtgat gcggccgcag
     7561 ggctacgaag tggtcgtgga tgacgaggtg gccgacgaag cagcccgact gctcggaacg
     7621 ggagtctgat atggcacgcg cagctggaat cgacctcggc acgacgaact cggtcatcgc
     7681 ggcctgggag gccggtcagt cccaggtcgt cccgaacgcc gagggcgcgc gtacgacgcc
     7741 gtcggttgtc gcattcctcg agaacggtga gcggttggta ggccagctgg cccgccggca
     7801 ggcgatcctc aaccccaagg ggacgatcta ctcggtcaaa cgcttcatcg gccgccgctt
     7861 cgaggaggtc gaggaggagg cgaagcaggt cacctacgac gtggtgcccg acgagaacgg
     7921 gctcgcccgg atcaaggtgc gcgacaagct gtacgcgccg gaggagatct ccgcgctgat
     7981 ccttcgcaag ctcgccgacg acgccgggcg ccacctcggc gagcgcgtca ccgaggccgt
     8041 ggtcacggtg ccggcgtact tcaacgacgc ccagcggacc gccacccggg atgcggggaa
     8101 gatcgccggc ctcgaggtac tgcggatcat caacgagccg acggcggcag cgctggcgta
     8161 cgggctggac aaacgccagc acgagacggt actggtgttc gacctcgggg gcggcacgtt
     8221 cgatgtgagc atcctcgacg tcggcgacgg cgtggtggag gtacgcgcga ccgcgggtga
     8281 cacccacctc ggcggcgacg acttcgaccg gcgcatcgtc gaccacctgg ccgagcagtt
     8341 ccagaaggag aacagcatcg acctgcggaa ggacccgcag gcgttgcagc ggctctacga
     8401 ggccgcggag aaggcgaagg tggagctgag ctccgtcacc cagacccagg tcaacctgcc
     8461 tttcatcact gccgacgcga acggtcccaa gcacctcacc atgacgctga tgcgctcgac
     8521 gttcgaggag ctcacccgcg atctcgtgga ccgcacgatg gagccggtcc ggcgcgcaat
     8581 gtcggacgcc aaggtcgggc ccgacgacat cgacgaggtc attctcgtcg gcgggtcgac
     8641 ccggatcccg gcggtgcagg ccctggtgcg gcgcctcacg ggtggcaagg aaccgaacat
     8701 gaccgtcaac cccgacgagg tcgtcgctgt cggtgcgggg atccaggccg gggtgctcaa
     8761 gggcgacgtg tccgacgtct tgctgctcga cgtcaccccg ctgtcgctgg gggtggagac
     8821 gcagggcggc gtcatgaccc gggtcgtcga ccgcaacacg acgatcccgg tccggcgcag
     8881 cgagacgttc tcgacggcgg cggacaacca gcccgccgtc gacgtggtgg tgctgcaggg
     8941 cgagcgcgag cgcgcggccg acaaccgtgt gctcggcagg ttccagctca ccgacatccg
     9001 gccggcaccg cggggggagc cgcagatcga ggtcgtctac gacatcgacg ccaacggaat
     9061 cctcaatgtc accgctcgtg acaaggacac ggcggcggag cagggcatca cgatctcgga
     9121 gagcaccaac ctggactcct ccgaggtgga tcggatggtg caggaggccg agcgcaaccg
     9181 gtcctcggac cagcagctgc gccaggaggt cgatgcgcgc aacgagctcg acagcctcgc
     9241 ctaccaggtg cagcgccggc tcgacgagct cggcgacgca gcgccgtcgc acgagcgggc
     9301 ccgcgccgag atgctcgtgg ccgacgcccg gcgtgcggtg gaggagcagg cgccgctgga
     9361 cagggtgcgt cagctgaccg gtgagctcca gcaggtgctg gccggcttga gcgcggtgtc
     9421 ggcctccgca ggagcgggcg gtgcgcaggg tggcggcagc gagggcgcca ccagcgagac
     9481 cgaggacgat gtgatcgacg ccgagttcga ccgtagctga ggctgtccga tgaccgaaca
     9541 agcacaggac aggacacaaa gccagcccgc cggcgtcgtc gccgatcagg ggggcgccgc
     9601 gccggaggag cgggggggcg cgggatcggg cacgccggcc gaaggatccg ctgaggaccg
     9661 gctcgacagt gggccggacg cggcggagct ggaggatcgc tggcggcgcg ccctcgccga
     9721 tctggacaac ctgcgcaagc ggtacgcccg cgagctcggg cgggagcgag aggccgagcg
     9781 tgagctggtg gcgtcggcat tcctgccggt gctcgacacc atcgacaggg ccctcgagca
     9841 cgccggcgcg gacccacgat cgatcgtcga gggcgttcgg accctgcgtg agcaggccct
     9901 cgcggtcctg gccgggctcg gttaccgccg cgaggacgag accggggtgc cgttcgaccc
     9961 ggcccgtcac gaggtagtgg gcgtggtcga ggccgacggc ggtgacgcgg cgccgggttc
    10021 ggtggcttcg gtcgtgcggc ccggttacgg cgcgcccggc cgtcagctgc ggccggcggc
    10081 ggtgaccgtg gcgcagactc cggggggcta gccgatggcc cgcgactact acgagacgct
    10141 cggggtgtcg cgcaacgcag gccccgacga gctgcagcag gcctaccggc ggctggccag
    10201 ggagaaccac cctgacgtca acagggaccc cggcgcggag gagcggttca aggaaatcaa
    10261 cgacgcctac cacgtgttgt ccgacccgtc gctgcgcaag cgctacgacc gcttcggcga
    10321 cgacttccgg caggtcccgg aggactggga ggagcgcgtc ggcgccgggg cgggccagtt
    10381 ccgcggagcg ccgggcggcg gtgccggcgg cggcggcggt cggaggtaca ggtggtcgcc
    10441 gggcggtgac ggcggtcccg acttcggcag ggactacggg ggcggcgact tcggcggtag
    10501 ggacttcggt ggtggtttcg gcggcgtcga catcgaggac ctcctcggcg ggatgttcgg
    10561 gggccgcggc cggggacgcg cgggccctgt cgtgggcgcc gaccaggagg cggagctctc
    10621 gctcacggtg gaggaggcct atcgcggcgg gcgcaggcag gtcaccctga acggcccgga
    10681 cgggccgcgc acctacacgg tgacgatccc gcgcggagtc accgacggca cccggatcag
    10741 gctggccggc gagggtggcg gcgggatggg cggcgccccg gcaggcgacc tgtacctcgt
    10801 ggtgcggctg ctgccgcacc cgcgctaccg cgtgcagggc aaggacatcg tggtcgacct
    10861 gcggctggcg ccgtgggagg gcgccctcgg agccacggtc ccggtcacga cacccggggg
    10921 cgagaccaag gtgaccgtcc cgccgggtac gtccacagga cggcggctgc ggctgcgcgg
    10981 ggagggcatg cccgacccgc gcggcaagcc gggcgacctc tacgccgaag tcaagatcat
    11041 ggtgccgccc cggctcaccg acgaggagcg gaggttgttc tccgaactcg ccgccgtctc
    11101 cacgttcgat ccgcgccgct ccggctccga cgaggggagg ggagcccgcc gatgaccgcg
    11161 cagtacttgc ctatccggta ccggccgacc cggggatcgg gacgtggctg gctgggtcag
    11221 gaggagttcg cgcgccgctg cggcctgcac cccgaactcg tccggcggtt cgtcgcgctc
    11281 ggcctggtgc ctgccgtgcg tgatgcggac ggagctctct ggttcagcac gtcacaggtt
    11341 gccgccgtcg cccgactgca gcggctgcgc gccgcgctgc cgctgaacta cgcagcgctc
    11401 gggctcgtcg tcgatctgct cgaccgcatc accgagctgg agaccgccct gcgcgtccgg
    11461 gggcacaccg aaccggtgcg cccaccggcc cgtccccagc agcccactgc catcgaccag
    11521 aagcggagcg agcgatggac ctgaaccgtc tgacccagat gtcccagcag gcgctctccg
    11581 ccgcgcagag cgtcgccact cgcgccggcc acacggaggt cgacggcgag cacctgctgc
    11641 tcgcgctgct cgaccagacc gatgggctgg tgccccgact gctcgtcgcg atgggcgtcg
    11701 acgagggtgc cctgctctcg gacctggagg ccgagctcgc gcgcaagccg cgcaccaccc
    11761 gcccggccgc cgacccgggg caggtctcga tcacgcagcg actggccagg gccctcgagg
    11821 ctgccgagcg cgaggcgcgt cggatgaagg acgagtacgt ctcggtggag cacttgctcg
    11881 tcgcgctggc cgaggagggt tccgcgacgg gagcgggccg gctgctcgcc aagcacggcg
    11941 tcacccgcga cgggttcctc gccgcgctga cccgggtgcg gggcaatcag cgcgtcacgt
    12001 cggccacccc ggagggcacc tacgaggcgc tcgtgaagta cggcatcgac ctcgtcgagt
    12061 cggcgcgctc gggccggctc gacccggtga tcggtcggga cgccgagatc cgccgggtgg
    12121 tgcagatcct gtcgcggaag acgaagaaca acccggtgct gatcggcgac cccggcgtcg
    12181 gcaagaccgc catcgtcgag ggcctggccc agcgcatcgt caacggcgac gtgccggagg
    12241 gcctacgcga ccggacgatc ttcgccctgg acatgggtct gctcgtggcc ggggcgaagt
    12301 accgcggcga gttcgaggag cggctgcagg ccgtgctggc ggaggtcaag ggggccgagg
    12361 gccggatcct gctgttcatc gacgagctgc acaacgtggt gggcgccggc gccgcggaag
    12421 gtgcgatgga cgccggcaac atgctcaagc cgatgctcgc ccgcggcgaa ctgcacatga
    12481 tcggcgcaac gacggtccag gagtaccgca agcgcatcga gtccgatgcg gcgctggagc
    12541 gccgcttcca gccggtctat gtcgacgagc cgaccgtcga ggacacggtg tcgatcctgc
    12601 gcgggctgcg cgagcggctc gaggtgttcc acggcgtccg gatcctggac ggggcgctga
    12661 tcgctgcggc gaccctgagc catcgctacc tcaccgaccg gttcctgccc gacaaggcca
    12721 tcgacctcgt cgacgaggcg tgcgcccggc tgcggaccga gatcgactcg atgcccgccg
    12781 cgctcgacga gatcacccgc aaggtgatgc ggctggagat cgaggaagcc gcgctcgcga
    12841 aggaggagga tcctgcgtcg gtcgcccggc tcgatcagct gcgccgcgag ctggccgacc
    12901 tgcgcagcga ggccgacgcc atgcgcgccc agtgggacgc ggagcggcag gcgatcaagc
    12961 gggtgcagga gctgcgggcg gagctggagc aggtccgccg ggagatcgag gaggcggagc
    13021 ggtcctacga tctcaaccgt gcggccgagc tccgctacgg cacgctcgcc gagctggagc
    13081 gcaggctgga ggccgagggg cagcggctcg ccgagaagca gggcgagcac cggcttctgc
    13141 gcgaggtggt cacggccgag gagatcgccg agatcgtctc ggtgtggacc ggtatcccgg
    13201 tgtcccggct gaccgagtcg gagcgggaga agctgctgcg cctcgacgag gtgctgcacg
    13261 agcgcgtggt cggtcaggac gaggcggtgc aggcggtcgc ggacgcgatc atccgggcgc
    13321 gttcgggggt caaggacccg cgacggccca ccggttcgtt catcttcctc ggcccgaccg
    13381 gtgtagggaa gacggagctg gccaaggcgc tggccgaggc gctgttcgac tccgaggaca
    13441 acatggtccg gatcgacatg agcgagtacc aggaacggca cacggtgtcg cggctggtcg
    13501 gcgctccgcc gggatatgtc ggctacgagg agggcgggca gctcaccgag gccgtgcgcc
    13561 gccgtccgta cgccgtggtc ctcttcgacg agatcgagaa ggcgcacgcg gacgtgttca
    13621 acacgttgct gcaggtgctc gacgacggcc ggctcacgga cgcgcaaggc cgcaccgtca
    13681 acttccgcaa caccgtgatc atcatgacgt cgaacatcgg gtcgcagtac atgctcgacg
    13741 gtgtcacccc ggacggccag ctcaagcccg acgcccgcga gctcgtgctg gccgagctgc
    13801 ggagtcactt ccggcccgag ttcctcaaca gggtcgacga caccgtgctg ttcaccccac
    13861 tcacgctgag cgagatcgag cacatcgtcg agctgatgct cgccgagctg cgctcccggc
    13921 tcgcggaccg caacctgaac ctggagatca gcgaggacgc ccgtctcttc atcgcgcggg
    13981 agggctacga cccggtgtac ggtgcccggc cgttgcgccg gttcatcgca cgcgaggtgg
    14041 agacccgcat cgcccgcgcg ttgctgcgtg aggggcccgc cgacggctcg acgctgcggg
    14101 tcgaggtcga gggcgatcag atcgtcgtca ctcatcaccc ggtgggagcg caggtatgag
    14161 cgccccggcg aagccgcgca cgagtgttgt cacctgcccg cactgcgggc aacgcaaccg
    14221 cgtgcccgcc gcggccgcga gcgtcccgag atgcgggaac tgccacaatg ccctgccctg
    14281 gatcgccgat gcgggcgacg acgacttctc cgaggtggcg gagcggtccc cgatcccggt
    14341 ggtggtggac ctgtgggcga cgtggtgcgg gccctgccgg atggtgagcc cggcactgga
    14401 gcagctggcc attgaacggg ccggcgacat caagctggtg aaggtcgaga tcgacgccgc
    14461 gccccggttg gcacagcggt tcgaggtgcg cgccgtcccg acgttgctgg tgatgcgcga
    14521 cagcgaggtg atcgctcggc agccgggcgc cgcgccggtg gcggcgctac gcaggtggct
    14581 ggaccaggcg ctgcagccga aggagggcga gacgcgatga gcgtgcgcaa tcagctccgg
    14641 cagctgtcga ccgggcgcgg taactggtgg aaggcggtta tccggtgacc atccccgtca
    14701 ggcccgaccc gcatctggcc ttcgcccgcg acgatgtcgt gccgcgcacc ccgcagggct
    14761 gcgaggactg tctgcggctc ggatcgccat gggtgcacct gcggctgtgc ctgacgtgcg
    14821 ggcacgtggg gtgctgcgat tcgtcgccga atcggcacgc gcgcgcgcac gccggactcg
    14881 tcggtcaccc gatcgtccga tcgttcgagc cgggagagga ctggcgctgg tgcttcgccg
    14941 acgaggcgtt cgcatgaggg cctccgcgac cggcgacacg tcgcggacct tacgacaatt
    15001 ccaggaaccg gtcgcaggag gtcgttgtgg atgagctggg gccggcgggg ccggcgctcc
    15061 gcccggtggg cgtccgggag agctcggttg ccgcggcgag gcatgcacgg ctgcccgagg
    15121 cggcgaccag gtacaccgtg gctgtcccgg tgcacggcgc agtggaggtc tcgcaccggg
    15181 cgcacaccgt ggaggccgat gcggcccggg cggccgtcct tcagcccgag ggcgactctg
    15241 acatgtcgtt cggcgacggc ttctcctgct acctggtcac gatcgacacc gggaagctga
    15301 aagatgtgct ggagcatcgg ctcgggcacg ccgtccgccg gccgcccgac ctgtcggaga
    15361 gcctggatct gcgaactccg gccgggcggg cctgggcccg cctcgccggg ttgctggcct
    15421 cctcgcccgt gctacggcac ccgctgctgg ccgacgcggt gcaggagatg ctgctggccc
    15481 gattgctgct cgccctcgat cacccgtatc gcgacgagct cgacgcaccg gtgcactcgt
    15541 ggggtccggg gccggcgcac cgaatggtcg acgcgatcga ggccagtcca cggcacccct
    15601 tcaccgtcgc ggagctttcc gacgtcgccg gcgtgagcgt gcccgcactg cgggagtgtt
    15661 gccggcgcca tctggacacc tcgcccaccc aacagcttcg ctcgatccgg ctgacccgcg
    15721 cgcgccgcga actgaccgag gccgatttcg ggtggaccag cgtcgccggc gtggcgtcgg
    15781 ggtgggggtt cacgaacacc gggcgtttcg tcgccgactt cgggaaccgc tacggggagc
    15841 cgccgtggca gacgctgcgc gggcccgcct acgcctgact cgggcagaga gcggcgcgag
    15901 ctatacatga gccctgcccc aaccggcggc ctggtgacgg tgttcgtgtg cggggacgtc
    15961 atgctcggcc gcggggtgga ccagatcctg ccgcacccgg atgccccggc cctccgggag
    16021 cggggcgtgc gggatgctcg gacctacgtc gagctggcgg aggcggtcaa cggtctggtc
    16081 cctcgttccg tcggcttctc ctggccgtgg ggcgacgcat tggcgttgct cgacgagctg
    16141 gctcccgatg tgcgggtggt gaacctcgag acgagcatca cccggcgtgg ccggttcgct
    16201 ccagggaagg gcgtccacta ccggatgaac ccggcgaacg tcccgtgcct cacagcagcc
    16261 cggccggatg cctgcgtgct ggcgaacaac cacgtcatgg acttcggtca gcggggtctg
    16321 aaggacacgc tcgaaacctt ggccggggca gggctgcgcg cggtgggagc gggacgcgac
    16381 gaggccgggg cgtggtgccc cgctgtcgtc tccctccccg gaggcggccg cctggtcatc
    16441 cacgcgttcg gaaccccgtc cagcgggatc cccgtgagtt gggcggcaag tgggcagcgg
    16501 ccgggcgtcg gcttcgtgcc cgagctttcc gacgcggccg ccgaccgggt gctcgaccgg
    16561 gtgcagcagc acgggggtca gctcgtcatc gcctcgatcc actggggatc caactggggc
    16621 taccgggtgt tccccgacca ggtgcgcttc gcgcaccggc tggtcgacgg tggggtggac
    16681 gtcgtgcacg ggcactcctc gcaccatccg cgccccatcg aggtgtaccg cgacaagctg
    16741 atcctctacg gctgcggcga cttcatcgac gactacgagg gcatcagcgg tcacgagggg
    16801 taccgggacg acctccgcct cgggtacttc gcgtcgatcg agcggggcac cggcagcatg
    16861 gcgggactgc ggatggtgcc cctccaggcc cggcggctgc ggctctgcca cgcctcgggc
    16921 aacgacgcgg cgtggctccg caccgttctc ggccgcatca gccaggactt cggtacgcgg
    16981 atagacctcg gaccggacgg gatgctcctc ctgcggtgca cggggacgga caggttctga
    17041 ccgggcggca ggcgctgcgg ggtcattgcc ggcccaggat gggtacccga ggttcgtgcc
    17101 acggcatgac cgagcgatgg gagggacctg cgatggctca gtgcgaggtg tgcggaaacg
    17161 actacgacat ggcgttcgag gtgcacgccc aggacgccgt gcacgtgttc gacagcttcg
    17221 agtgcgcgat ccatc
//
LOCUS       DASRSC010000287         5387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_443689,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000287 DASRSC010000000
VERSION     DASRSC010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5387
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443689"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VFE39_14220"
     CDS             <1..207
                     /locus_tag="VFE39_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259798.1"
                     /translation="IVLTLLDANQANQLAMLIHQTADTFNLGLSNLFLPADPKLGVVL
                     NYGTAALIWFAITTVVVRLVRRIP"
     gene            400..1665
                     /locus_tag="VFE39_14225"
     CDS             400..1665
                     /locus_tag="VFE39_14225"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET6259799.1"
                     /translation="MALVVQKYGGSSVQDADRIKKVAERIVRTHKEGHDVVVVVSAMG
                     DTTDELLDLADQVSPAPPPREMDMLLTAGERISNALVAMAIHALGAQARSFTGSQAGM
                     ITTSRHGDARIVQVNPQRLRDALDEGSIVLVAGFQGVSQQSKDVTTLGRGGSDTTAVA
                     LAAALHADVCEIYTDVDGIFSADPRIVPDAQRLSSITYEEMLEMAACGAKVLHLRAVE
                     YARRYDVPLRVRSSYNDKPGSLVSGSIEEIPVEQAIITGVAHDRSEAKITVTGVPDTP
                     GMAARIFRTVADAEINIDMVLQNVSHSGARRTDITFTLPRTDGARAVTALMAAQPEIG
                     FDEVLHDAEVGKVSLVGAGMRNHPGVTATFCEALSNAGINIETMNTSEIRISVICRVE
                     QLDDAVSALHAAFQLGGAEQAVVHAGSGR"
     gene            1662..2768
                     /locus_tag="VFE39_14230"
     CDS             1662..2768
                     /locus_tag="VFE39_14230"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET6259800.1"
                     /translation="MSEFTNESAGLGASGSGPVVAIVGATGAVGTTMIDIIDARPSVP
                     WSEIRLVASARSAGRVLRVRGQDVTVQALAPEVFDGVDVALFDVPDEVSAEWAPIAAS
                     RGAVAVDNSGAFRMDPDVPLVVPEVNADKTAERPKGIIANPNCTTLSMMAAMGALHRE
                     FGLEALVVASYQAASGAGQSGIDRLYAELAAVGGQEVGMRGGDVAQALAEAGLDPAAS
                     PFPAPLALNVVPWAGSLKDDGWSSEELKVRNEARKILGIPDLKVSATCVRVPVVTTHS
                     LAVHATFATEVSVEAARKVLGEQPSIVLRDDPAAGEWPTPADAVGGDPTWVGRIRQAL
                     DFPNTLELFLCGDNLRKGAALNTYEIAETVAARL"
     gene            complement(2837..3787)
                     /locus_tag="VFE39_14235"
     CDS             complement(2837..3787)
                     /locus_tag="VFE39_14235"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET6259801.1"
                     /translation="MAAVVFTALVAVFTVPDLLFGLDERSPFAQLVSFRPWVLAGVVA
                     LLVLLLVVMVFERRVLPFVAGVLVVLLVGCGVMLPRVVPDPVPTTGTPFTVLAFNTLE
                     GGSDVQQLAALIDQQRPDVVALSEAGVPYSDKLAPLVAPLGYHLHTSIGPRRRDVGGV
                     TAVVSDRLGDVQVRIGNETSSFPYVELSGAGLGGMRVVAFHSVAPVPGSVPEWRSDLA
                     LLKQWCAGPTPAIVAGDFNATLDHSVLRNAISGCEDAAAQRGAGLVPTWGPTPRSRDL
                     LGPQIDHVIANDGIAAETFEVHDIAGSDHRAILTRLRLPH"
     gene            complement(3894..5150)
                     /locus_tag="VFE39_14240"
     CDS             complement(3894..5150)
                     /locus_tag="VFE39_14240"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259802.1"
                     /translation="MQATEEATAGLAGYRAVLGLPGVAPLTGIAFLARIPTSAAAITL
                     TLHVVLTLDHGYAAAGLVGASATVGMAIGAPLLGRLVDRRGLRTMLSLTLCAEAVFWT
                     VAPWLSYPALLVGALLGGLLGMPVYSVVRQSLAAMVLPRLRRPAFALDSMSVEMSYII
                     GPAVGTLLALQVSTSTAMWVVGAGFVVSGVALWLLDPPTRAVESRADTQPGRVREWLD
                     LRLFAALLATTAAVVLIFGTELSMIAGLQTSGQPAAIAVVNAVWCLASLTGGFLYGAA
                     RRSPSLFVLVAAMGVATLPVALGASWWSYALLLVPAGLLCAPSLAASSEAVSALAPEH
                     ARGLVTGLHGSAITVGAAIITPISGLLIDVASPAVAVVAVGSAGIAVAGTAALLARRG
                     GRRRLPLSDPAPVPSVVGPSVDPQPI"
     gene            5247..5387
                     /locus_tag="VFE39_14245"
                     /pseudo
     CDS             5247..5387
                     /locus_tag="VFE39_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700362.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
BASE COUNT          859 a         1891 c         1933 g          704 t
ORIGIN      
        1 atcgtgctca cgcttctcga cgcgaaccaa gccaaccagc tcgcgatgct gatccaccag
       61 accgccgaca cgttcaacct gggcctgtcg aacctgttcc tgcccgcgga tcccaagctc
      121 ggtgtcgtgc tgaactacgg cacggccgca ctgatctggt tcgcgatcac cacggtggtc
      181 gtccggctgg tgcgtcgcat cccctgacct gttgtggcct ccgccaggcc acgcggcgca
      241 gccaccctgt ggtgtctgcc attcccatcg gcagccgctg tcacggccgg tagggtggga
      301 aagcgaccag cacctcgggt gaagcgctcc ggcgcggccc ggtgggcacg gtgagcccga
      361 cagtgtcgcg ccgcgcgaca ccaggacagg aggtcggcgg tggccctcgt cgtgcagaag
      421 tacggcggat cgtcagtcca ggacgcggac cggatcaaga aggtcgccga gcggatcgtt
      481 cggacccaca aagagggcca cgatgtcgtc gtggtggtgt cggcgatggg cgacactacc
      541 gacgagctgc tcgacctcgc cgaccaggtg tcccctgccc cgccgccccg cgagatggac
      601 atgctgctca ccgcgggcga acgtatctcc aacgcgctcg tcgcgatggc gatccatgcg
      661 ctcggtgcgc aggcccgctc gttcaccggc tcgcaggccg ggatgatcac gacgtcccgg
      721 cacggcgacg cacgcatcgt ccaggtcaac ccgcagcggc tgcgcgacgc gctcgacgag
      781 ggctcgatcg tgctcgtcgc cgggttccag ggcgtgagcc agcagtcgaa ggacgtcacc
      841 acgctcgggc gcggcgggtc cgacaccacg gcggtcgcgc tcgccgcggc gctgcatgcc
      901 gacgtctgcg agatctacac cgacgtcgac ggcatcttca gcgctgaccc gcggatcgtt
      961 cccgacgccc agcgcctctc cagcatcacc tacgaggaga tgctggagat ggctgcgtgc
     1021 ggggcgaagg ttctgcacct gcgcgctgtg gagtacgcgc gccggtacga cgtcccgctt
     1081 cgggtgcgta gttcctacaa cgacaagccg gggtcgctcg tcagcggctc gatcgaggag
     1141 attcccgtgg agcaggcgat catcaccggg gtcgcacacg accggtccga agccaagatc
     1201 acggtgaccg gggtgccgga cacacccggc atggccgccc gcatcttccg caccgtggcc
     1261 gacgccgaga tcaacatcga catggtgctg cagaacgtca gccactcggg cgcacggcgc
     1321 accgacatca cgttcacgct gccccgcacc gacggtgcgc gtgccgtgac cgcactcatg
     1381 gcggcgcagc cggagatcgg cttcgacgag gtgctgcacg acgccgaggt cggcaaggtc
     1441 tcgctggtcg gggcaggcat gcgcaaccac ccgggagtca ccgccacgtt ctgcgaggca
     1501 ctctcgaacg ccggcatcaa catcgagacc atgaacacct ccgagatccg gatctcggtg
     1561 atctgccggg tggagcagct cgacgacgcg gtgagcgccc tgcacgccgc gttccagctc
     1621 gggggagcgg agcaggccgt ggtgcacgct gggagcggac gatgagcgag ttcacgaatg
     1681 agtcggcggg gctgggtgcc tcgggcagcg ggccggtcgt cgcgatcgtc ggtgccaccg
     1741 gagccgtcgg aaccacgatg atcgacatca tcgacgcccg cccgtcggtg ccgtggtcgg
     1801 agatccggct ggtggcctcg gcccggtcgg cgggccgggt cctgcgggtg cgcgggcagg
     1861 acgtcaccgt gcaggcgctc gctcccgagg tcttcgacgg ggtcgacgtc gcgctgttcg
     1921 acgtgcccga cgaggtgagc gcggagtggg cgccgatcgc ggcctcccgc ggggccgtgg
     1981 ccgtcgacaa ctccggggcg ttccgcatgg atcccgacgt cccactcgtg gtgcccgagg
     2041 tcaacgcgga caagaccgcc gagcggccca aggggatcat cgccaacccc aactgcacca
     2101 cgctgtcgat gatggccgcg atgggtgcgc tacaccggga gttcggtctg gaggcgctcg
     2161 tcgtcgcctc ctaccaggcc gcctctgggg cggggcagag cgggatcgac cggctctacg
     2221 cggagctcgc cgccgtcggc ggccaggagg tcgggatgcg cggcggcgac gtcgcgcagg
     2281 cactggccga agccggcctc gaccccgccg cgtcgccgtt cccggcgcca ctcgccctca
     2341 acgtcgtgcc gtgggcgggc tcgctcaagg acgacgggtg gtcgtcggag gagctcaagg
     2401 tccgcaacga ggcccgcaag atcctgggca tccccgacct caaggtctcg gccacctgcg
     2461 tgcgggtccc cgtggtcacc acgcactccc tcgccgtgca cgccaccttc gccacggagg
     2521 tctccgtgga ggccgcccgc aaggtcctgg gcgagcagcc ctcgatcgtg ctgcgggacg
     2581 acccggcggc gggagagtgg cccacgcccg ccgacgccgt gggcggcgac ccgacctggg
     2641 tcggccggat ccggcaggcg ctggacttcc cgaacactct cgagctgttc ctgtgcggcg
     2701 acaacctgcg caagggtgcc gcgctcaaca cctacgagat cgcagagacg gtggcagcac
     2761 gtctctagcc gctccgacgc gcggatttcc ggcgtcctgc gtcggcgctg tgacgatctg
     2821 ctggtgggct cgcacctcag tgtgggagcc ggagccgggt gaggatcgcc cggtggtcgc
     2881 tgcccgcgat gtcgtgcacc tcgaacgtct ccgccgcgat gccgtcgttg gcgatcacgt
     2941 ggtcgatctg cggtccgagc aggtcgcggc tgcgcggtgt gggcccccac gtcggcacga
     3001 ggcctgcgcc gcgctgggcc gcggcatctt cgcaaccgga gatcgcgttg cgcaggacgg
     3061 agtggtcgag cgtggcgttg aagtcgccgg cgacgatcgc cggggtcgga cccgcgcacc
     3121 actgcttgag cagcgcgagg tccgagcgcc actccggcac ggacccgggc accggcgcca
     3181 ccgagtggaa cgccaccacg cgcatgccgc cgaggccagc tccgctcagc tcgacgtagg
     3241 ggaacgacga ggtctcgttg ccgatccgca cctgcacgtc accgagccgg tcggacacga
     3301 cggcggtgac accaccgacg tccctgcgcc gcgggccgat cgaggtgtgc aggtggtatc
     3361 ccagcggcgc gacgagtggt gcgagcttgt cggagtaggg aacccccgcc tcgctcagcg
     3421 ccaccacatc cggccgctgc tggtcgatca gcgcggcgag ctgctggaca tcgctcccgc
     3481 cctccagggt gttgaaggcg agcacggtga acggggtgcc ggtggtgggc acggggtcgg
     3541 gaacgacgcg cggcagcatg accccgcagc cgaccagcag cacaacgagg accccggcga
     3601 cgaacggcag cacacggcgt tcgaacacca tcaccacgag caacagcacg agcagcgcca
     3661 cgacgccggc gaggacccac ggccggaacg agacgagctg ggcgaacggg ctgcgctcgt
     3721 ccaggccgaa cagcaggtcg ggcaccgtga acaccgccac gagcgccgtg aacacgacgg
     3781 cggccacgta gcgcaggatc cttctgcggc gatgcggagg cgccggtggc ggcggcggtg
     3841 tgtccgcgtc ctggagccgg ggcggcgatg cggtcatcgc cacgaagtct gccctagatc
     3901 ggttgaggat cgactgaggg tcccacgacc gagggcaccg gcgcgggatc ggacaggggc
     3961 aggcgtcgcc gcccgccgcg gcgggcgagc agcgctgcgg ttccggcgac cgcgataccg
     4021 gcggacccga ctgccaccac cgcgactgcg ggagacgcga cgtcgatcag cagcccggag
     4081 atcggggtga tgatcgcggc gcccaccgtg atggcggatc cgtgcagccc ggtgacgagc
     4141 ccgcgcgcgt gctcgggcgc gagtgcgctg acggcctccg agctggccgc gagcgacgga
     4201 gcgcagagca gcccggcggg taccagcagc agggcgtagg accaccacga ggcgcccaac
     4261 gccaccggca gtgtggccac gcccatcgcg gcgaccagca cgaacagcga cggggatcgg
     4321 cgggccgcgc cgtagaggaa gcccccggtg agcgaggcca ggcaccacac cgcgttgacg
     4381 acggcgatag ccgccggctg cccgctggtc tgcaggcccg cgatcatcga gagctcggta
     4441 ccgaagatca gcacgacagc cgcggtggtg gctagcagcg cggcgaacag gcgcaggtcg
     4501 agccactcgc ggacccggcc cggctgcgtg tcggcgcggc tctccaccgc cctggtgggc
     4561 gggtcgagca gccagagtgc gacgccggag acgacgaacc ccgcgccgac aacccacatc
     4621 gccgtgctgg tcgagacctg caatgcgagc agggtgccga cagccgggcc gatgatgtag
     4681 gacatctcga ccgacatcga gtcgagtgcg aatgcagggc ggcggagccg tgggagcacc
     4741 atcgccgcga gtgactgccg caccaccgag tacaccggca tcccgaggag gccgccgagc
     4801 agcgctccga ccagcagtgc cgggtaggac agccagggtg cgaccgtcca gaacaccgcc
     4861 tcggcgcaca aggtgaggga cagcatggtg cgcagccccc gccggtcgac cagcctgccg
     4921 agcagcggcg ccccgatggc catcccgacc gtggcggacg cgccgaccag ccccgcggcg
     4981 gcgtagccgt ggtcgagggt gagtaccacg tgcagcgtga gtgtgatcgc ggccgccgac
     5041 gtcgggatcc tggccaggaa ggcgatgccc gtgagcggag ccacgccggg caacccgagg
     5101 acagctcggt agccggccag gcctgcagtg gcctcctcgg tcgcttgcac ccgctcatgg
     5161 tgacccggag gaccgacgga actcgaacga gttctgtcca acgtcaaact tgactgattc
     5221 cagaaaagct gttcgactgg tcggcggccg gctcgcccgc tcggtacgag acgcgcgccg
     5281 gcgacaacca ccaccacctg gtctgccgct cgtgcgggcg caccaccgat gtggactgcg
     5341 tggtcggtgc ccggccctgt ctcgcccctg gcgacgacgc gggctac
//
LOCUS       DASRSC010000288        10965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4043854,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000288 DASRSC010000000
VERSION     DASRSC010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10965
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4043854"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..117
                     /locus_tag="VFE39_14250"
     CDS             <1..117
                     /locus_tag="VFE39_14250"
                     /inference="COORDINATES: protein motif:HMM:NF020071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HET6259803.1"
                     /translation="FWTTGYPLVRAELRGRYPKHDWPEDPLTATPTRRPRRR"
     gene            complement(130..582)
                     /locus_tag="VFE39_14255"
     CDS             complement(130..582)
                     /locus_tag="VFE39_14255"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6259804.1"
                     /translation="MKDGFELGCDGPQTLVVGVDGSDTGWRAFHYALGQARRQGARVV
                     AVFADNLQAATFAGAGALALPLVESSADEYREELRGAVEGLGAEHGVAVRFVVAVADP
                     VLALTRVAEEVRADAILVGASTRAAHRLFGSVAVRAVRSGRWPVTVVP"
     gene            802..1245
                     /locus_tag="VFE39_14260"
     CDS             802..1245
                     /locus_tag="VFE39_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020632126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6319 family protein"
                     /protein_id="HET6259805.1"
                     /translation="MSTPQETTTTRRPTSPPEPPTPAPATDTAAAQPEPAGGAEPESG
                     KPRRGRPKGSTARTTRTIELTLTVSGTADGDWQAELKQGTTWLSRGLPVTATAVARAA
                     QELHAELAAPIEAVIGAAREQRAARVAALEAELDQARKALAEIEV"
     gene            1265..2686
                     /locus_tag="VFE39_14265"
     CDS             1265..2686
                     /locus_tag="VFE39_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015103750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6259806.1"
                     /translation="MPGIDPADLQTCLRVLAEVDALPPEHPDAVRIRRATAGIYKSVK
                     LRRRAAKREAVAAADDAVTATTATGAPGRVDDETQGLPLVSTATGATAGTLLRSRACY
                     TCKNRYHVVDAFYHQLCPPCAALNRSRRDARTDLRGRRALLTGGRAKIGMYIALRLLR
                     DGAHTTITTRFPNDAVHRFAAMSDSADWLHRLSVVGIDLRDPAQVVGLADSVAAQGTL
                     DILVNNAAQTVRRSPGSYAALVEAESAGPGPAGELVEITTFDHVRNERPAAIAGGPRQ
                     TPTALAGLALTARSASPERIAAGTAIDAGGLLPDTAAVNSWTQRVEEIDPLELLEVQL
                     CNQTAPFILVSKLRPALAASLARRKYVVNVSAMEGQFSRAYKGPGHPHTNMAKAALNM
                     LTRTSAGEMLESDGILMTAVDTGWITDERPHPTKLRLADEGFHAPLDLVDGAARVYDP
                     IVRGEAGEDVHGCFLKDYAPTHW"
     gene            2823..3029
                     /locus_tag="VFE39_14270"
                     /pseudo
     CDS             2823..3029
                     /locus_tag="VFE39_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020497576.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
     gene            3171..3404
                     /locus_tag="VFE39_14275"
     CDS             3171..3404
                     /locus_tag="VFE39_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259807.1"
                     /translation="MKIDWKSLLVVGVVSLAASVAIVVLVALALVGLSARSGAARPGG
                     GASALSPAAGTTLACVSLLAAVAIVGYGLYLVV"
     gene            complement(3370..3646)
                     /locus_tag="VFE39_14280"
                     /pseudo
     CDS             complement(3370..3646)
                     /locus_tag="VFE39_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684995.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3665..4540
                     /locus_tag="VFE39_14285"
     CDS             3665..4540
                     /locus_tag="VFE39_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6259808.1"
                     /translation="MTISGPTHTLIQLTDLHLVAEGETLPGGVDTAALLGRALQAVAG
                     AAVGPDGGGPSALVLTGDLTEHGRPEQYRRLRAILEPAAERLGAPLVLAAGNHDDRAA
                     LRQHLLGEPRSAEPFDHVVRIGALRIVVLDSTVPGYAHGALRPGQLAWLRAQLAEPAP
                     AGTVLTLHHPPLPSASPLAASIPLVHPEELGAAITGTDVRLVLAGHTHVASAGTLSGV
                     PVWTGGPLATTVDGLAPGATLRTLGAPSVSRIDLFTDGLLTTSVPIAAEVVADVPGEK
                     MEPRLAALREAAGVA"
     gene            complement(4659..5810)
                     /locus_tag="VFE39_14290"
     CDS             complement(4659..5810)
                     /locus_tag="VFE39_14290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HET6259809.1"
                     /translation="MLPLPLDGILVVSCEQAVAAPFATRQLAELGARVIKVERPGDGD
                     FARAYDTTVRGLSSHFVWLNRSKESLALDLKADAEAGADAVLPRLLARADVFVQNFAP
                     GAAERLGLGHEALRARFPRLITCSISGYGPSGPYRDAKAYDLLIQSEAGLVSVTGTPE
                     EPAKSGIPAADIGAGMYAFSGILAALYDRERTGVGTHLEISLFDALVEWMGFPLQYTA
                     GSGAPPQRAGTSHPAIAPYGTFTVGDGTEVVLGIQNEREWAAFCGVVLQRPELAADER
                     FRTGALRVEHRPALHAEIDAVFAACTGAEVTARLATARIAHARRREVAEVLEHPQLVA
                     RERWAEVGSPAGPLPALLPPITLPGRPPRMDPIPDVGEHTQAILRWLDD"
     gene            complement(5865..8798)
                     /locus_tag="VFE39_14295"
     CDS             complement(5865..8798)
                     /locus_tag="VFE39_14295"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HET6259810.1"
                     /translation="MSPDETATQLWSEEQLIADVEAGPQGPQVGAFFDFDGTLIDGYS
                     LGAFARHHLRSGHVFPADIGRMLLIGLRGLTTEEDFEDFFLLGMRAWTGRHEDELTEL
                     GEQLFAQGIAGSLYPEAWRLVEAHRRAGHTVVLASSATRFQVEPAARAMGVDHVLVTP
                     VEIENGLCTGRPGGPPLWRAGKAAAVQAFAQQHDVDLVESYAYSNGDEDVPFLAATGR
                     ARALNPGPGLAAEAARRNWPIGRFRSRGRPGARELVRTVGAVGGTLGGFAAGVAVGAL
                     NSIAGEGTRRDAVDLGLTLAGELGTALGGVRVDVRGAEHLVAARPAVFLFNHQSQLDV
                     LILAKLLRSNFTGVVKKEAANVPGFGLAFRLADVAFVDRTDTTAAKAALEPAVQRLRE
                     GISLVIAPEGTRSATPALGRFKKGAFHVAMQAGVPVVPIVIRNAGELMWRGASTLREG
                     TVQVTVLPPIPTAGWSVEELDERVEEVRGRYVETLAHWGGPAAPSGAVAATAAASGRV
                     TAPPDPSPLRWGRAREMNALETAMWRGEAADPRLRSNVTLLELLDPAPEWERLVAAHE
                     WASRMVPRMRQRVVEPALGVGAPNWVTVGDLDLDHHLSRVRLPAPGSMRQLLDVVQEF
                     AAAPFDRDRPLWRAMLVEGLEGGRAGYVVTTHHSATDGLGAVQLMGMLHSRSAEHDPH
                     RPEPPAPQPELPASPAALLTGQVGRTVRGVPGEVLRRGAGLLGALARPEAVAGRAFDA
                     VRSLGHAVAPPPSGGSPLLADRSGQWHFEVLDVGLDALKAGAKAAGGSLNDGFLAAVL
                     GGFRRYHQHFGAELGTIPIAIPISLRTEDDPQGGNRFTGARLAGSLAEPDPTARIRAV
                     REFVLTARAETRVDTVGLLAPALTWLPGSVLGAASARLISANDVQVSNLPGVGHPIYI
                     AGSRITRMYPFGPLPGCAATITLLSHDGTCCIGVNLDPAAVTEPSVLVAGVRAGLDEV
                     VGLAG"
     gene            complement(8788..>10965)
                     /locus_tag="VFE39_14300"
     CDS             complement(8788..>10965)
                     /locus_tag="VFE39_14300"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF002886.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase"
                     /protein_id="HET6259811.1"
                     /translation="AVLPVKGPALAQPLVDSGEDAVVAAVRVAWLPRERNGQRRVRWA
                     DVVSLRNPRHPPASQQARIVRREPDRVALVVAEPATVGSLRARFAASRSDGSGATSVR
                     AFGDFVARQAALALDRAERALVGDRYKVPRQIVEAIEGSPQFRREVTELAASLGRPEA
                     EVAAKAQADLHGLVASMSPMAVDLLTSALRPLHARAWDVQVDTAGLEKLRECNRRHAL
                     VFLPSHRSYADPLLLADVLAEHDFPRNHVLGGDNLRFWPVGPLARRAGVVFIRRSFGD
                     DEIYKLAVREYFAFLLAKRFNLEWYMEGGRSRTGKLRPPRYGLLANVAEAVERGRTDD
                     VHLVPVSITYDQLREVSAMAAEQVGAPKKGEGLAWLARYAKAQLSPIGTAYVRFAEPI
                     SLRGALGAQLDVQDPDARRLALQKVAFEVAVGINRVTPVVATALVTLALLGVRDRALT
                     LGQVQRVLEPVRGYVRARGCAIDTLRPDPLDTAAGLRRVLGALAASKVVTVYTGGEEP
                     VYAIERGQHLIAAFYRNSAIHHFVDRAIAELMLLNDPADRWDEAMRLRDLLKFEFFFP
                     DRENYRAQLSAELGKLDRSGETADGEQVLASADFLIAHRVLRSFLDAQLVVAERLAAR
                     APRHPFAEKSFLDECSGVGQQMLLQGRLHGPESLSRELFGSALKLAANRDLLGCEDDR
                     SAREELAQRRHAFAAQLRDVVSRVIRIDEIDAESRREAVGVEP"
BASE COUNT         1575 a         4224 c         3752 g         1414 t
ORIGIN      
        1 ttctggacca ccggctaccc cctggtgcgc gcggagctgc gcggccgcta ccccaagcac
       61 gactggccgg aggacccgct gaccgcgaca ccgacgcgcc ggccgcgccg acgttgacac
      121 cttctgccct cagggcacga cagtgaccgg ccaccgcccg gaccgcaccg cccgcacggc
      181 gaccgacccg aacagccggt gggctgcgcg ggtgctcgcg ccgaccagga tggcgtcggc
      241 ccggacctcc tcggccaccc gggtgagcgc cagcaccggg tcggcgacgg cgacgacgaa
      301 gcggacggcc accccgtgct ccgccccgag cccttcgacg gcgccccgta gctcctcgcg
      361 gtactcgtcc gccgagctct ccaccagggg aagggcgagc gccccggcgc ctgcgaacgt
      421 cgctgcctgg aggttgtcgg cgaacacggc gaccacccgg gcaccctgcc ggcgggcctg
      481 gccgagggcg tagtggaagg cgcgccatcc ggtgtcggag ccgtccacgc ccaccaccag
      541 ggtctgcgga ccgtcgcagc cgagctcgaa gccgtccttc atgacccact ctccggtgag
      601 gcaacgcctg agggtgcgca ccgtgacccc ggacggcccg ggccggcggg acgacctggc
      661 gcggcgcgca ccctcccctt tatatagcgt agctaaacac ttttccagtg ctctcgcgcg
      721 agccgacccc gccgccgtcg aacatgtgtt ctaccatcaa cctggttcgc cgaccgtcac
      781 gagagggagc acgccacacc gatgagcacc ccccaggaaa caacaacgac ccggcggccc
      841 accagccccc cggagccgcc cacgcccgcg ccggcaaccg acacggcagc agcacagccg
      901 gagccggcag gcggcgccga gcccgagtcc gggaagcccc gccgcggccg cccgaagggc
      961 tccaccgccc gcaccactcg caccatcgag ctcaccctca cggtcagcgg caccgctgac
     1021 ggcgactggc aggcggagct caagcagggc accacgtggc tcagccgtgg cctgccggtc
     1081 acggccaccg cggtcgcccg cgccgcccag gagctgcacg cggagctggc cgcacccatc
     1141 gaggcggtga tcggcgcggc cagggagcag cgggccgctc gtgtcgcggc gctggaggcg
     1201 gagctggatc aggcgcgcaa ggcgttggcg gagatcgagg tctgagcgcc cgtacccttc
     1261 ggaggtgccc ggtatcgacc ccgctgatct gcagacctgc ctccgggtgc tcgccgaggt
     1321 cgacgcgcta ccccctgagc acccggacgc ggtgcgcatc cggcgagcca ccgcggggat
     1381 ctacaagtcg gtgaaactcc gccgccgggc cgccaaacgg gaagcggtcg ccgccgccga
     1441 cgatgcggtc acggccacca ccgcgacggg tgctccaggc cgggtcgacg acgagacgca
     1501 gggcctaccc ctcgtctcca ccgccaccgg cgccacggcg ggcactctgc tgcgctcccg
     1561 agcctgctac acctgcaaga accggtatca cgtcgtcgac gcgttctacc accagctctg
     1621 cccgccctgc gctgccctca acaggagcag gcgcgacgcc cgcaccgacc tcagagggcg
     1681 ccgtgccctg ctcaccggtg gccgggcgaa aatcgggatg tacatcgcgc tacgactgct
     1741 ccgcgacgga gcgcacacga cgatcaccac gcgattcccg aacgacgcgg tgcaccggtt
     1801 cgccgccatg tccgacagcg ccgactggct gcaccggctg agcgttgtcg gcatcgacct
     1861 ccgcgatccg gcacaggttg tcggcctcgc cgactcggtg gcggcccagg gcacgctgga
     1921 cattctggtg aacaacgccg cgcagaccgt ccgccgttcc ccaggctcct acgccgcgct
     1981 ggtcgaggcc gagagcgccg gccccggacc tgccggcgag ctggtcgaga tcaccacctt
     2041 cgaccacgtg cgcaacgagc gtccggcagc gatcgcgggg ggaccgcgac agacgccgac
     2101 cgcgctcgcc gggctcgccc tcacagcgcg cagcgcgtca ccggagcgga tcgccgcggg
     2161 caccgccatc gacgcgggcg gcctgcttcc cgacaccgct gcggtgaaca gctggacgca
     2221 gcgggtggag gagatcgacc cgctcgagct tctcgaggtg cagctgtgca accagaccgc
     2281 gcccttcatc ctggtcagca agctgcgccc ggcgctggcc gcgtccctcg cgcggcggaa
     2341 gtacgtggtg aacgtcagcg cgatggaggg acagttcagc cgcgcctaca agggaccggg
     2401 gcatccccac accaacatgg cgaaggcggc gctcaacatg ctcacccgca ccagcgcggg
     2461 cgagatgctc gagagtgacg gcatcctgat gaccgctgtc gacacaggct ggatcacgga
     2521 cgagcggccc catccgacca agctgcgact ggccgacgaa ggcttccacg cgccgctcga
     2581 tctcgtcgac ggcgcggcgc gcgtgtacga cccgatcgtg cgcggtgagg cgggcgagga
     2641 cgtgcacggc tgcttcctca aggactacgc ccccacccac tggtaaatgc gcgatcggtt
     2701 tatatctgtc gcattcgtcc cggataacac agaagtcgcg cactattttt gatcgacggt
     2761 tcatgtactg ttccccgaac acctcgtagc gtggcgctca tgaggcggag ggaccggcgc
     2821 ctatgaatgt tccgatcacc gtggtggcga tggtggtcac cgcgctcgcg ttggacttta
     2881 caaatggatt ccacgacgtg gccaatgcga tggccacctc gatcgccacg ggcgcactgc
     2941 ggccgaaggt cgcggtgctg attgccggag tgttgaacct cgtcggcgcc ttcctttccg
     3001 tccaggtcgc gaaggccggg gtggtcgcgc tggtgtccac cttcctggcc gcgggcatct
     3061 acgcagccgc gcggccccga cccgtcaccg cgcacaacgt caacgacgtt cctacggcgg
     3121 gcgagcccgt cggcgccagc gcctgacgcc cgagaaccaa cgaaggacac atgaagatcg
     3181 actggaaatc gctgctggtg gtgggggtcg tgtcgctggc ggcgtctgtc gccatcgtcg
     3241 tgctggtcgc cctcgcactc gtcgggctct ccgcccgctc cggggcggcg cgtccgggcg
     3301 ggggggcgag cgcgctcagc ccggccgccg gcacgacgtt ggcctgcgta agcctgctgg
     3361 cggcggtcgc tatcgtcggc tacggcctct acctggtcgt ctgaccgttg ctgttccgcg
     3421 gggggtcggg ctccggtgac cgcccggtga ccagagcctg tccgtcacgg aagaggacgc
     3481 cgtcggcgca gcggcttcga cctggccgcc ggcgaactgc tcgatgtgaa tgcgcgcgta
     3541 cagcccgcca tgcgcgagca gttcggcgtg aatgccgcgc tccccgaccc ggcccgcctc
     3601 cacggcgagg atcacgtcgg cgccggatgg tggacagccg gtgcgccgac ccacgatgga
     3661 gagcgtgaca atctccggtc ccacccacac cctgatccag ctcaccgacc ttcacctggt
     3721 cgccgagggc gagaccctcc ccggtggcgt cgacaccgcc gccctcctcg gccgcgcgtt
     3781 gcaggccgtc gccggcgccg ccgtggggcc cgacggaggc ggtccgagcg cgctcgtact
     3841 caccggcgac ctcaccgagc acgggcgccc tgagcagtac cggcggctcc gcgcgatcct
     3901 ggagcctgcg gcggagcgcc tcggcgcccc gctcgtcctc gccgccggta accacgacga
     3961 ccgcgctgcg ctgcgtcagc atctcctggg cgagccgcgt tccgcggaac cgttcgacca
     4021 cgtcgtccgc atcggggcgt tgcggatcgt cgtcctggac agcaccgtgc ccggctatgc
     4081 ccacggtgca ctgcggccgg gacagctcgc atggctgcgc gcccagctcg ccgagcccgc
     4141 ccccgccggc accgtgctca cgctgcacca cccgccgctg cccagcgcgt cgccgctcgc
     4201 cgcctccatc ccgctggtgc accccgagga gctgggtgcc gcgatcaccg gcacggacgt
     4261 ccggctggtg ctcgcgggcc acacgcacgt cgccagcgcg ggcacgctga gcggcgtccc
     4321 cgtctggacc gggggtccgc tcgccaccac cgtcgacggg ctcgcgccgg gcgccacgct
     4381 ccgcacgctc ggagcgccgt cggtgagtcg gatcgacctg ttcaccgacg gcctgctcac
     4441 gacgtccgta ccgatcgctg ccgaggtcgt ggcggacgtg cccggcgaga agatggaacc
     4501 gaggctcgcc gcgctccgtg aggccgccgg cgttgcttga tcccaccaaa ccggcgtcga
     4561 gcagcagtcg gggccgcgcg cccgttcggg tcctcgcagg ggccgaggga tctgcgaaga
     4621 ccgggcacgg cgcgcggatc agccgacgat gctcccgctc agtcgtcgag ccagcggagg
     4681 atggcctgcg tgtgctcccc cacgtcaggg atcggatcca tgcggggcgg tcggccgggc
     4741 agcgtgatcg gcggcaggag cgcgggcagt ggtccggcgg gtgagccgac ctcggcccag
     4801 cgctctcgag ccacgagctg ggggtgctcg agcacctcgg ccacctcgcg acgccgggcg
     4861 tgggcgatgc gcgcggtcgc cagccgagcc gtcacctcgg ctccggtgca cgcagcgaac
     4921 acggcgtcga tctcggcatg cagggccggg cggtgttcca cccgcagcgc cccggtccgg
     4981 aaccgctcgt cggcggccag ctccggacgc tgcaggacga ccccgcagaa cgccgcccac
     5041 tcccgctcgt tctggatccc cagcacgacc tcggtgccgt cgcccacggt gaacgtcccg
     5101 tagggcgcga tcgcgggatg gctcgtgccg gcccgctgcg gcggtgcgcc gctgccagct
     5161 gtgtactgca gcgggaaccc catccactcg accagcgcgt cgaacaggct gatctccagg
     5221 tgcgtaccga cgcccgtgcg ctcgcggtcg taaagcgccg cgaggatccc ggagaacgcg
     5281 tacatgcccg ccccgatgtc cgcggcgggg atcccgctct tcgcgggctc ctcgggcgtc
     5341 ccggtgaccg agacgagccc ggcctccgac tggatcagca ggtcgtaggc cttcgcgtcg
     5401 cggtacgggc cggacgggcc gtagccggag atcgagcagg tgatcagccg ggggaaccgc
     5461 gcgcgtaggg cctcgtgccc cagcccgagc cgctcagccg ccccgggcgc gaagttctgc
     5521 acgaagacgt cggcgcgggc cagaagccgg ggcagcacgg cgtccgcgcc cgcctccgcg
     5581 tcggccttga ggtcgagcgc caggctctcc ttggagcggt tgagccagac gaagtgactg
     5641 gacaggccac gcacggtcgt gtcgtaggcg cgggcgaagt cgccgtcgcc cggccgctcc
     5701 accttgatca cccgagcgcc gagctcggcg agctggcggg tggcgaacgg cgcggctacg
     5761 gcctgttcgc aggagacgac gaggatcccg tccaggggca ggggcagcac tctcgcatca
     5821 tgcctgcacc ggcaaccgga ccgtgcgctg ctgcctcgcc ccgctcagcc cgccagcccc
     5881 accacctcgt ccagacccgc ccgcaccccg gccaccagca ccgacggctc ggtgacggcc
     5941 gcggggtcga ggttgacgcc gatgcagcac gttccgtcgt gggagagcaa tgtgatcgtc
     6001 gcggcgcacc ccggcagcgg accgaacggg tacatgcgcg tgatccgcga gcccgcgatg
     6061 tagatcggat ggcccacccc tggcaggttg ctgacctgca cgtcgttggc cgagatcagc
     6121 ctggccgacg cggcacccag tacggaaccg ggcagccagg tcagtgcggg cgcgagaagg
     6181 cccaccgtgt ccacgcgggt ttcggcacgc gcggtgagca cgaactcccg caccgcccgg
     6241 atccgcgccg tcggatccgg ctccgcgagc gagcccgcga gccgggcacc ggtgaaccgg
     6301 ttgccgccct gcgggtcgtc ctcggtgcgg aggctgatcg ggatggcgat cgggatcgtg
     6361 cccagctcag caccgaagtg ctggtggtaa cggcggaagc cgcccagcac cgcggccagg
     6421 aatccgtcgt tcagtgaccc gcctgcggcc ttcgccccgg ccttcagcgc gtccagcccg
     6481 acgtcgagga cctcgaagtg ccattgcccg ctgcggtcgg cgagcagcgg cgatccaccc
     6541 gacggcggcg gcgccacggc gtggccgagc gagcgcaccg cgtcgaaggc ccgcccggcg
     6601 accgcctccg gccgcgccaa cgcgccgagc agcccggcac cgcggcgcag cacctcgccc
     6661 ggcactcccc ggacggtgcg cccgacctgc ccggtcaaca gcgccgcagg cgacgcgggc
     6721 agctccggct gcggcgccgg gggctccggc cggtgcgggt cgtgctctgc cgagcggctg
     6781 tgcagcatgc ccatgagctg cacggcgccg agcccgtcgg tcgcgctgtg gtgcgtcgtc
     6841 acgacgtagc ccgcgcggcc gccctcgagg ccctccacca gcatcgcccg ccagagcggc
     6901 cggtcgcggt cgaacggggc cgccgcgaac tcctgcacca cgtcgagcag ctggcgcatc
     6961 gacccagggg cgggcagccg cacccgcgac aggtggtggt cgaggtccag gtcgccgacg
     7021 gtcacccagt tcggcgcgcc gaccccgagg gcgggctcca ccacccgctg gcgcatccgc
     7081 ggcaccatcc gcgaggccca ctcgtgtgcg gcgacgagcc gctcccattc cggtgccgga
     7141 tcgagcagct ccagcagcgt gacgttggag cgcagtcgcg ggtccgcggc ctcgccgcgc
     7201 cacatggccg tctccaacgc gttcatctcg cgcgctctgc cccatctcaa cgggctcggg
     7261 tcgggcggtg cggtgacgcg tccggacgca gcggccgtcg cagcgaccgc gccggacggc
     7321 gccgccggtc cgccccagtg cgcgagggtc tcgacgtagc gaccgcggac ctcctccacg
     7381 cgctcgtcca actcctcgac cgaccacccg gccgtgggga tggggggcag cacggtgacc
     7441 tgcacggtgc cctcccgcag cgtcgacgcc ccgcgccaca tcagctcgcc cgcgttgcgg
     7501 atgacgatcg gcacgaccgg gacgcctgcc tgcatcgcga cgtggaacgc gcccttcttg
     7561 aacctgccca gcgccggtgt ggccgaccgc gtgccctcgg gggcgatgac cagcgagatc
     7621 ccctcgcgca ggcgttgcac cgcgggctcg agcgcggcct tcgccgccgt ggtgtcggtg
     7681 cggtccacga aggcgacgtc ggcgagccgg aacgccagcc cgaacccggg gacgttggcc
     7741 gcctccttct tcacgacccc ggtgaagttg ctgcgcagca gcttcgcgag gatcagcacg
     7801 tcgagctggc tctggtggtt gaacaggaac accgcgggcc gcgcagccac caggtgctcc
     7861 gcgcctcgga cgtcgacccg caccccgccc agcgcggtac cgagctcccc ggccagggtc
     7921 agcccgaggt cgaccgcgtc gcggcgcgtc ccctcccccg cgatgctgtt gagcgccccg
     7981 accgcgacgc ccgcggcgaa cccgccgagc gtgccgccca ccgcccccac cgtgcggacc
     8041 agctccctgg cccccggccg gccgcgcgac cggaagcgcc cgatcggcca gtttctccgg
     8101 gccgcctcgg ccgcgagacc gggcccgggg ttcagcgcgc gtgcccgacc tgtggcggcc
     8161 aggaacggca cgtcctcgtc gccgttggag taggcgtagc tctcgaccag gtcgacgtcg
     8221 tgctgctgtg cgaacgcctg caccgcggcg gccttgcccg cccgccacag cggcggcccg
     8281 cccggtcggc ccgtgcacag cccgttctcg atctccacgg gcgtcaccag cacgtggtcc
     8341 acgcccatcg cgcgcgccgc gggctccacc tggaacctgg tggccgagga cgccagcacc
     8401 accgtgtgcc cggccctgcg gtgtgcctcc acgagccgcc atgcctccgg gtagagcgac
     8461 ccggcgatcc cctgcgcgaa gagctgctcc cccagctccg tcagctcgtc ctcgtggcgg
     8521 cccgtccacg cccgcatgcc gagcaggaag aaatcctcga agtcctcctc cgtcgtcagc
     8581 ccgcgcaggc cgatgagcag catccgcccg atgtcggccg ggaacacgtg ccccgagcgc
     8641 aggtggtgac gggcgaacgc gccgagcgag tacccgtcga tcagggtgcc gtcgaagtcg
     8701 aagaacgcgc cgacctgcgg gccctgcgga cccgcctcga cgtcggcgat cagctgctcc
     8761 tcgctccaga gctgcgttgc cgtctcatca gggctcaact cccaccgcct cccgccgcga
     8821 ctcggcgtcg atctcgtcga tccggatcac cctgctgacg acgtcgcgca actgggcggc
     8881 gaacgcatgc cgccgctgcg ccagctcctc ccgcgccgac cggtcatcct cgcagccgag
     8941 gagatcccgg ttggccgcca gcttcagtgc gctcccgaag agctcgcggg agagcgactc
     9001 cggcccgtgc agcctcccct gcaagagcat ctgctgcccg acgccactgc actcgtcgag
     9061 gaacgacttc tcggcgaacg ggtggcgcgg ggctcgcgcg gccagccgct cggccaccac
     9121 cagctgcgcg tccaggaacg accggagcac ccggtgggcg atgaggaagt cggcgctcgc
     9181 gaggacctgc tcgccgtccg cggtctcccc cgaccggtcc agcttcccta gctcggccga
     9241 cagctgcgcc cggtagttct cgcggtcggg aaagaagaac tcgaacttca gcaggtcgcg
     9301 caggcgcatc gcctcgtccc agcgatcggc cggatcgttg agcagcatga gctcggcgat
     9361 cgcccggtcg acgaagtggt ggatggcact gttgcggtag aacgcggcaa tgaggtgctg
     9421 cccgcgttcg atcgcgtaga cgggctcctc cccgccggta tagaccgtga cgaccttcga
     9481 cgcggccaat gcacccagca cccggcgcag acctgccgcc gtgtccaggg gatccggccg
     9541 gagcgtgtcg atcgcgcagc cacgggcccg gacgtacccg cgcacgggct cgagcacgcg
     9601 ctgcacctgc cccagcgtca gggcccggtc gcggaccccc agaagcgcca gggtgaccag
     9661 cgcggtcgcg accacgggcg tcacgcggtt gatcccgacg gccacctcga acgccacctt
     9721 ctgcagcgcc agcctccgcg cgtcgggatc ctgcacgtcc agctgtgccc cgagcgcccc
     9781 ccgcagcgag atcggctcgg cgaaccggac gtacgcggtt ccgatgggcg acagctgcgc
     9841 cttcgcgtac cgcgccaacc aggccagccc ctcccccttc ttcggcgccc cgacctgctc
     9901 ggccgccatc gccgacacct cgcgcagctg gtcataggtg atcgagaccg gcaccaggtg
     9961 gacgtcatcg gtgcggccac gctcgacggc ctccgccacg ttggccagca gcccgtatcg
    10021 cggcgggcgc agcttcccgg tgcgggaccg gccgccctcc atgtaccact ccaggttgaa
    10081 ccgcttcgcc agcaggaacg cgaagtactc gcggacggcg agcttgtaga tctcgtcgtc
    10141 gccgaagctc cgccggatga agacgacccc tgctcgcctg gccagcggac ccaccggcca
    10201 gaaccgcagg ttgtccccac cgagcacgtg gttgcggggg aaatcgtgct cggcgagcac
    10261 atcggccagg agcagcgggt cggcatagga ccggtgactg ggcaggaaca cgagcgcatg
    10321 ccgcctgttg cactcgcgca gcttctccag accggcggtg tccacctgga cgtcccacgc
    10381 cctggcatgc agcggccgca gcgcggacgt gagcaggtcg accgccatcg ggctcatcga
    10441 ggcgacgagg ccgtgcaggt cggcctgcgc cttcgcggcc acctccgcct cgggacgccc
    10501 cagcgaggcg gcgagctccg tgacctcacg gcggaactgc ggactgccct cgatcgcctc
    10561 gacgatctgc cggggcacct tgtaccggtc gcccaccagg gcgcgctccg cccgatcgag
    10621 cgccagcgcg gcctgccggg cgacgaagtc accgaacgcc cgcactgatg tcgcgccgga
    10681 cccgtcggaa cggctggcgg cgaaccgggc ccgcagcgac cccacggtgg cgggctcggc
    10741 caccacgagc gccacccggt ccggctcccg tcgcacgatc cgggcttgct ggctcgccgg
    10801 tgggtgccgc ggattccgta aggacacgac gtcggcccag cgcacccggc gttgtccatt
    10861 gcgctcgcgg ggcagccacg ccacccgtac cgcagcgacg acggcgtcct caccggagtc
    10921 gaccagcggc tgggccaacg ccgggccctt gaccggcagg accgc
//
LOCUS       DASRSC010000289         6434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4371688,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000289 DASRSC010000000
VERSION     DASRSC010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6434
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4371688"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..744)
                     /locus_tag="VFE39_14305"
     CDS             complement(70..744)
                     /locus_tag="VFE39_14305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010223782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET6259812.1"
                     /translation="MNLDTAVFDVDGTLVDTNYHHALAWYRAFRRHGITLPVWRLHRA
                     IGMGGDQLVGAVAGDEVEARWGDAVRAAWAEEFAPMLEEVQPFEGVAELLGEVRRRGF
                     RVVLASSGKPEHVEHYLGLFDGKELADAWTTSEDVQRTKPAPDLLEAALRAVDGSAGV
                     MIGDSVWDFRAAAALGAPGYAVRSGGFSVDELCTAGALDVFDSPAELLHQLDETLLSG
                     SGQRVS"
     gene            complement(812..1225)
                     /locus_tag="VFE39_14310"
     CDS             complement(812..1225)
                     /locus_tag="VFE39_14310"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HET6259813.1"
                     /translation="MRLLPRGIDLGRHDDVPGREREVIPVAASINAWGSILEQLGGNH
                     AKETSLITNPDTDPHDYEPTPADGRVIAGSKLFVENAPPPTAGGGDGFAMLVPWSTST
                     GSTARGWPTSSAGARRRGLELDPPGLGAWAVTNAG"
     gene            complement(1708..3024)
                     /locus_tag="VFE39_14315"
     CDS             complement(1708..3024)
                     /locus_tag="VFE39_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6259814.1"
                     /translation="MSYANLDLAASAPALQAVTDHVSQVLPYYASVHRGAGYASQVCT
                     AVLEDARATVGRFVGARDDDVVVFTRSTTDSLNLLARCVPGSVLTLDIEHHANLLAWR
                     GGHHRVISAAETIEATLDALRTALTAQPTALLAVTGASNVTGEVLPIARIVAIAHAAG
                     TRVVLDAAQLAPHRRIDIAATGIDYVALSGHKLYAPYGTGALVGRRDWLDTAPPHLAG
                     GGAVRAVTHRNDTADATWAPAPHRHEAGTPNVLGAAALAAACRTLEPVLDTTAPEHER
                     ALLRRLTDGLDAIPGVHPLTIWTDAPDRVAVVSFTVDGHPAGRVAAYLSAEHGIGVRD
                     GKFCAHPLLARIAGGADAVRASCGISTTTDHIDRLLTALHTLVTRGTTWGYAVIDSRW
                     RPTPDPRDPDPFRLNSRPGATAASWACGEQDRVGRTFASSATTVNA"
     regulatory      complement(3190..3303)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00634"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class IV; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00634"
     gene            complement(3609..5108)
                     /locus_tag="VFE39_14320"
     CDS             complement(3609..5108)
                     /locus_tag="VFE39_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET6259815.1"
                     /translation="MDPEEFRRLGHAVVDWIAEYRARIADLPVRADVEPGWVREQLTP
                     GLLSPGLPEEPQPLSGLLDEIDRVVVPASMHWQHPGFFGYFPANVSLHSLLGDLLSGG
                     LGAQGMLWSTSPAATEVEQVLLDGLADALGLPPSFTFAGGGGGSIQDSASSAALVALL
                     AALHRSAPDWQERGVDGTERVYVTAETHSSLAKAVRVVGLGSRALRIVEPSPGSLAMS
                     PAALAAALRADVDAGLRPVLVCPTIGTTGTGAIDPVRGVARAAAAHGAWVHVDAAWAG
                     VAALCPEHRDLLDGVELVDSFCTDAHKWLLTAFDASMLWVQDATALPAALSITPEYLR
                     NAASESGAVVDYRDWQVPLGRRFRALKLWAVVHGHGLSGLRAHIRSHVALAAELAGWV
                     RDDPGFTLTVPPSLALVCLRVVTGAGPDADDRATRAVLEGVNAAGSEFLTHTVVDGHY
                     SIRVAIGSVATEFPHVRALWERLQADAARYRQNSSVDGVDPAPPRSTHS"
     gene            complement(5117..>6434)
                     /locus_tag="VFE39_14325"
     CDS             complement(5117..>6434)
                     /locus_tag="VFE39_14325"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6259816.1"
                     /translation="PAAEAPPDLSGDWHGSIETPGAPLQIGVRLAGDGGTFDLPAQGL
                     RALALQDMRFDGHAVTFRLPDVPGGAAFDGTLAEDGRITGNYSQGGQSVAFSLTRGPM
                     PPSGRPQEPQPPFPYRSENVTYPSGDLTMAGTLTLPDDAGPFAAVLMITGSGAQDRDE
                     TIAGHKPFLLLADTLTRAGYAVLRVDDRGVGGSGGDLQQASFDDLAGDVLAGLAFLRG
                     RPDIDPTRIGLFGHSEGGYLAPLAAQRAAGGVAFVVLMAGPAVSGEEVLVLQNQLILQ
                     QAGAPPDQIEAQINYVRQLSGLLRVEDYEGARQLARARVAEQTAALAENQRPTPEQVE
                     AQLPATPAFRAFLTFDPAPALRALNVPVLAVYGGKDLQVPARQSEPVLRTLLANNRDA
                     TIQTFPGLNHLMQPTTTGAVTEYSTNETTVAPEVLDLVSAWLRARF"
BASE COUNT          927 a         2162 c         2450 g          895 t
ORIGIN      
        1 cgccgtggcc gtgatcgccc tcggcgacct cgtgtgggtg ctctaccgga agcggcgggg
       61 cgagcccggc taggacacgc gctgaccgct tccggagagg agcgtctcgt cgagctggtg
      121 gaggagctcc gccggggagt cgaagacgtc caacgccccg gccgtgcaca gctcgtcgac
      181 cgagaaacca ccgctgcgca cggcgtagcc gggagcgccg agtgcggccg cggcccgaaa
      241 atcccacacc gagtcgccga tcatcacgcc cgcgctgccg tcgacggcac gcaaggccgc
      301 ctccagcaga tcgggggcgg gcttggtgcg ctgcacgtcc tcggaggtgg tccaggcgtc
      361 ggcgagctcc ttgccgtcga acaggccgag gtagtgctcg acgtgctcgg gcttaccgga
      421 gctggccagc acgacgcgga agccccggcg gcgcacctcg ccgagcagct ccgccacccc
      481 ctcgaagggc tgcacctcct ccagcatcgg ggcgaactcc tcggcccacg cggcccgcac
      541 ggcgtcgccc caccgtgcct cgacctcgtc gcccgccacg gccccgacga gctggtcgcc
      601 gcccatgccg atcgcccggt gcagccgcca gacgggcagc gtgatgccgt ggcgccggaa
      661 cgcccggtac caggcgagcg cgtggtggta gttggtgtcg acgagcgtgc cgtcgacgtc
      721 gaacacggcc gtgtcaagat tcacgtgcgg cgagtacccc gatgtgtggg cggtgacgca
      781 ccggggagac atcgtgctcg cggtcctgga cctaccccgc gttcgtgacg gcccaggcgc
      841 caagccccgg cggatcgagc tccagacctc gccgacgcgc acctgccgaa ctggtcggcc
      901 acccccgggc ggtcgaaccc gtcgatgtgg accacggcac caacatcgca aagccgtcgc
      961 cgccgccagc ggtcgggggt ggagcgttct ccacgaacag cttcgagccg gcgatgaccc
     1021 gtccgtcggc cggggtcggc tcgtagtcgt gcggatcggt gtccgggttg gtgatcagtg
     1081 acgtttcttt cgcgtggttg ccgccgagct gctcgaggat cgagccccac gcattgatgc
     1141 tcgcggccac cgggatcacc tcccgctccc ggccggggac atcgtcatgg cggccgaggt
     1201 cgatgccgcg ggggaggagg cgcaaccggc cacgcccaca accaacgtgg ccgcggcgag
     1261 gggaacaaca agggaacgac cagatatgcg cgtggtccag gctccagtcc gatggctcgt
     1321 cgccccgagg atgtgcacgt agcggctgcc gactcccagg acgaagaaga cgtagatccg
     1381 ttcagggtta ctgcgcggtc gacatggaag aagtccacgg ccagcatggt cgaggcctgt
     1441 gccgaaggaa ctgcttccgt gtcgtatcgg tgtgccggga tggtgccgga ggtatccgcc
     1501 gcggcttgag gatcctgcgg atcgtggatg ctccgacgcg atggcgagct tgagcagttc
     1561 gccgctcacc gccgcggcgc gggccgatca ggacgtgcag agacggcgtt ccgcccgcga
     1621 gttcgtcccc aatctgatag gtctgcgccc ccgctgctgg cccaccgcgc tgctcagccc
     1681 gattgattcc gtcggccgaa ctgcgagcta cgcgttcacg gtcgttgccg acgaggcgaa
     1741 ggtgcgcccg acccggtcct gctcgccgca tgcccagctg gctgcagtcg cgcccggtcg
     1801 gctgttcagc ctgaaggggt cggggtcgcg ggggtctggg gtgggtcgcc agcggctgtc
     1861 gatgacggcg tagccccagg tggtgccccg tgtgacgagg gtgtgcaggg cggtgaggag
     1921 tcggtcgatg tggtcggtgg ttgtggagat gccgcagctg gcgcggacgg cgtcagcacc
     1981 gcccgcgatg cgggcgagca gcgggtgggc gcagaacttg ccgtcgcgga cgccgatgcc
     2041 gtgttcggcg gagaggtagg cggcgacgcg tccggcgggg tggccgtcga cagtgaagct
     2101 cacgacggcg acgcggtcgg gcgcgtcggt ccagatggtc agggggtgca cgccggggat
     2161 ggcgtcgagg ccgtcggtga ggcggcggag cagcgcgcgt tcgtgctcgg gcgcggtggt
     2221 gtcgaggacg ggttcgaggg tgcggcaggc ggcggcgagt gcggcggcac ccagcacatt
     2281 gggggtaccg gcttcgtgac ggtgtggggc gggtgcccaa gtggcgtcgg cggtgtcgtt
     2341 gcgatgggtg acggcgcgga ctgcgccgcc gccggcgagg tggggcgggg cggtgtcgag
     2401 ccagtcgcgg cgtccgacaa gggctccggt gccgtagggg gcgtagagct tgtggccgga
     2461 gagtgcgacg tagtcgatgc cggtggcggc gatgtcgatg cggcggtgtg gggcgagttg
     2521 ggcggcgtcg agcaccacgc gggttccggc ggcgtgggcg atggcgacga tccgggcgat
     2581 cgggagcacc tcgccggtca cgttcgacgc gccggtaacg gccagcagcg ctgtgggttg
     2641 agcggtgaga gcggtacgaa gggcatcgag ggtggcctcg atggtctcgg cagcggatat
     2701 cacccggtgg tggccgccgc gccaggccag gaggttggcg tggtgctcga tatcgagggt
     2761 caggactgag ccgggcacgc accgggccag caggttcagc gagtcggttg tggaacgtgt
     2821 gaagacgacg acgtcgtcgt cacgggctcc gacgaaccga ccgacggtgg cgcgggcgtc
     2881 ttcgagcacc gcggtgcaca cctgggacgc gtatcccgcg ccgcgatgca cgctggcgta
     2941 gtagggcagc acctgcgaga cgtgatcggt cacggcttgc agggcgggcg cgcttgcggc
     3001 gaggtcgagg ttcgcgtagc tcacgaagcg gccgtcgacg agggggactc gaaggtctgc
     3061 accgacgacg gcaggcaacg ggaccggcgt tgtgtcatca acatcgcaga aggtgatatc
     3121 gacagcagga gcagtgggag cgatggacat ggtcgtcctc cggatctgag gacccggatc
     3181 tggcgatcgg gtccgcgctt gcccacaacg ggatcgtgtc ggcctggtcg tcacccggag
     3241 caccccgccg cggaggaggg ttgccggcca gcgagccggg gcttgacgct ggcgctcatg
     3301 acctggatcc gacgttacgg cggccgtcgg tcgatcgtca agccgccgta ccgaccgcgc
     3361 ctccgaccgc cgtcgccacc gaccaggcgg tggcgagcag cgggcggcgc ggcggtggac
     3421 cgagccgcta cccctggttc gtgacctcgg cgcgcagctg ccgctcctgt tcgatgagct
     3481 ccggcgtggc gttcggggcg aagtcctcgg cctcgaagac ccggcggatc tcgatctcct
     3541 cctcgccgaa ggcggggtcg tacgggcagc gcggttctcg tcacgggatg cgggcacttc
     3601 gtgcggctct acgaatgcgt cgaccgcggg ggcgcgggat cgacaccatc gaccgaagag
     3661 ttctgccggt accgtgccgc gtcggcctgc agccgctccc acaaggccct gacatgcgga
     3721 aactccgtcg cgaccgatcc gattgccacg cgaatggagt aatgaccgtc gacgacggta
     3781 tgggtgagga actccgaccc cgcggcgttg acgccctcga gcacggcccg agtggcgcgg
     3841 tcgtcggcgt ccggccctgc cccggtgacg acgcgcaggc acaccaacgc gagcgacggc
     3901 gggacggtca gggtgaaccc ggggtcatcg cgcacccacc cggccaactc cgccgccagc
     3961 gccacgtgcg agcggatgtg tgcccgtagc cccgagagcc cgtggccgtg cacgacggcc
     4021 cacagcttga gcgcgcggaa gcggcgcccg agcggtacct gccagtcgcg gtagtccacg
     4081 accgcgccgg actccgaggc cgcgttgcgc aggtactccg gagtgatcga gagtgctgcc
     4141 gggagggcgg tggcgtcctg cacccagagc atcgaggcat cgaacgcggt caggagccac
     4201 ttgtgggcgt cggtgcagaa ggagtcgacg agctcgacgc cgtcgagcag gtcgcggtgt
     4261 tcggggcaga gagctgccac cccggcccac gcggcgtcga catggaccca ggccccgtgc
     4321 gctgcggcgg cgcgggccac cccgcgcacc gggtcgattg cgccggtacc ggtcgtgccg
     4381 atcgtcgggc agacgagtac cggccgcagc ccggcgtcga cgtcggcccg cagcgcggcg
     4441 gccagcgcgg cgggtgacat cgccagcgag ccgggggagg gctcgacgat ccgcagcgcc
     4501 cgcgacccga gcccgacgac ccgcaccgct ttcgcgagcg aggagtgcgt ctcggcggtg
     4561 acgtacacgc gctcggtgcc gtcgacgccc cgctcctgcc agtccggggc gctgcggtgc
     4621 agggccgcga gcaacgccac cagtgcggcg gaggacgcgg agtcctggat cgacccacct
     4681 ccgccccccg caaaggtgaa ggaggggggc agcccgagcg catcggccag cccgtcgagc
     4741 agcacctgct cgacctcggt cgcggccggc gaggtggacc agagcatccc ctgcgcgccg
     4801 agcccgccgg acagcaggtc gcccagcagc gagtgcaatg acacgttggc cgggaagtac
     4861 ccgaagaaac cggggtgctg ccagtgcatg ctcgccggca ccaccacgcg gtcgatctcg
     4921 tcgagcagac cggacaacgg ctgcggttcc tcgggcagcc cgggggacag caatcccgga
     4981 gtcagctgct cgcgcaccca ccccggctcg acatccgccc gcacgggaag gtcggcgatc
     5041 cgggcgcggt actccgcgat ccagtcgacg accgcgtggc cgagcctccg gaactcctcg
     5101 gggtccatat cgggcatcag aaccgcgccc gcagccatgc ggagaccagg tcgagaacct
     5161 ccggtgcgac cgtggtctcg ttcgtcgagt actcggtgac cgcgccggtc gtggtcggct
     5221 gcatcaggtg gttgagcccg gggaacgtct gaatggtcgc gtcccggttg ttcgcgagca
     5281 gcgtgcgcag caccggctcg ctctgcctcg ctggcacctg caggtccttg ccaccgtaga
     5341 cggcgaggac cggcacgttc agcgcgcgga gcgcgggcgc cgggtcgaag gtgaggaacg
     5401 cccggaacgc gggcgtggcg ggaagctggg cctcgacctg ctccggggtc ggacgctggt
     5461 tctcggccag ggcggcggtc tgctcggcga cgcgggcgcg ggcgagctgc cgggcgccct
     5521 cgtagtcctc gacgcgcagc agcccgctga gctgacgaac gtagttgatc tgggcctcga
     5581 tctggtccgg tggtgcgccc gcctgctgga ggatcaactg gttctgcagc accagcacct
     5641 cctcgccgga gacggccggg cccgccatca gcacgacgaa cgcgacgccg ccggccgcgc
     5701 gctgggcggc cagcggagcg aggtagccgc cctcgctgtg cccgaacagc ccgatccgcg
     5761 tggggtcgat gtcgggacgg ccccgcagga acgcgagccc ggcgagcaca tcgcccgcca
     5821 ggtcgtcgaa gctcgcctgc tggagatcgc cgccggagcc gccgacaccg cggtcgtcca
     5881 cccggagcac ggcgtacccg gcgcgggtga gcgtgtcggc gagcagcagg aacggcttgt
     5941 gtcccgcgat ggtctcgtcc cggtcctgcg cgccgctgcc ggtgatcatc agaacggccg
     6001 cgaaaggccc ggcgtcgtcg ggaagggtga gcgtcccggc catcgtcagg tcgccgctcg
     6061 ggtaggtcac gttctcgctg cggtagggga agggcggctg cggttcctgc ggccggcccg
     6121 aggggggcat gggtccccgg gtcagcgaga acgcgacgct ctggccgccc tggctgtagt
     6181 tcccggtgat ccgcccgtcc tcggcgaggg tgccgtcgaa cgcggccccg ccaggcacgt
     6241 cgggcaggcg gaacgtgacg gcatgcccgt cgaaccgcat gtcctgcaac gccagcgcgc
     6301 gcagtccctg ggcgggaagg tcgaacgtgc cgccgtcgcc tgccaggcgc actccgatct
     6361 gcaacggcgc cccgggtgtc tcgatgctgc cgtgccagtc gccggagagg tccgggggtg
     6421 cttccgctgc cggt
//
LOCUS       DASRSC010000290         4269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4044997,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000290 DASRSC010000000
VERSION     DASRSC010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4044997"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="VFE39_14330"
     CDS             <1..1267
                     /locus_tag="VFE39_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6259817.1"
                     /translation="LVALAALPVLLVATAVFRRRSTVAYAEARERVSIVNADLQENVT
                     GVRIAQAYVREERSAAAFGERSLAYRHSRLRAQRYIATFFPFVAFLSDVAQAVVLGVG
                     AARLASGDLTPGVLTAFLLYLGLFFAPVQQLSQVFDGYQQARVGLSRISDLLRTPTSV
                     PPEPASDPLEVPHRLRGEVELRDVGFTYAGTQAPALEHVSLRVAPGETIALVGATGAG
                     KSTVVKLLARFYEVGTGAVLVDGVDVRRYPLGDYRRRLGIVPQEPHLFTGDVAANIAY
                     ARPDATPAEIEAAARAVGALDLVRGLPGGFRHPVGERGQGLSAGQRQLVALARAELAD
                     PDLLLFDEATAALDPATEAAVLAAGDRVIARRTAFVVAHRLATAARADRIVVLDDGRI
                     VEVGPHAELLAAGGHYARLWRAGELEPAA"
     gene            1634..1903
                     /locus_tag="VFE39_14335"
     CDS             1634..1903
                     /locus_tag="VFE39_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020131788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259818.1"
                     /translation="MPAGPYDDLIAHLVRSTPLSTGEAARVIAEVVGYFSEQLPDFVR
                     RRHAEMKRRGLTNEQIFTALGAELATRRVAPPPLSERQLRRMVYG"
     gene            1915..3741
                     /gene="glmS"
                     /locus_tag="VFE39_14340"
     CDS             1915..3741
                     /gene="glmS"
                     /locus_tag="VFE39_14340"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET6259819.1"
                     /translation="MCGIVGYVGPQDAAPILLEGLGRLEYRGYDSAGLAVSAKGGLKV
                     RKVSGRVADLAAQLPPRLKGSPGIGHTRWATHGEPNETNAHPHLDAAGRIAVVHNGII
                     ENADELRAKLAADGVEFVSQTDTEVVAHLVAAAFTAGAGDLEQAVRQALRQVVGAYGL
                     AVLDAQHPDRIVVARNGSPVLLGVGEKEMFVASDVAALIGYTRQVVYLDDGELATITA
                     GGYRTYTLDDRSTAKSPSTIDWDVVGAEIGNHAHYLIKEIQEQPRTIERALKGRIDER
                     FDTAHLGGLNLSVREAREVKRVKILGCGSACYAGELGAQLIEDLARVPASAEAASEFR
                     YRNPVVEPDTLYVAVSQSGETIDTLAAVQELQRKGGRVIGIVNVVGSTIARQVDGGIY
                     LHAGPEMSVAATKSFTSTVTAFALLALHLGRIRDLSPEQGRRIIAGLNALPGQITEIL
                     EQSDAIAEVAAEIAQSRSVMFVGRRRGWPVAREGAQKLKEISYVHAEAYPSAELKHGP
                     LALISPEMPTVAIVPDDDLLDKNISTLSEIKARKGPVIAIAHRELPADLADRTLVIPK
                     GEPELDPILLSIPLQILAYHAAIVLERDVDKPRNLAKSVTVE"
     gene            complement(3728..>4269)
                     /locus_tag="VFE39_14345"
     CDS             complement(3728..>4269)
                     /locus_tag="VFE39_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MSMEG_0567/Sll0786 family nitrogen starvation
                     N-acetyltransferase"
                     /protein_id="HET6259820.1"
                     /translation="SASASGRRIRPARPVNVLVGVDPVHCRPVRDVAGLAAHHRIRHA
                     VFVEEQGLFPEGDEDAHDARTDVVHVLGLHAGRPAGAVRLFPTGPGEWLGDRLAVLPE
                     FRSANIGAPLVRFAVAAAGARGARLMRAHVQVANERFFHRLGWRTDGPVETYVGHPHV
                     PMCIEPGALCPWLAGTTPR"
BASE COUNT          612 a         1541 c         1525 g          591 t
ORIGIN      
        1 gctggtggcg ctcgccgcac tacccgtgct gctggtcgcc acggcggtgt tccgcaggcg
       61 gtccacggtg gcgtacgcgg aggcccgcga gcgggtcagc atcgtcaacg ccgacctgca
      121 ggagaacgtc acgggtgtgc gcatcgcgca ggcctatgtc cgcgaggagc gcagcgccgc
      181 ggcgttcggc gagcggagcc tggcgtaccg acactcccgg ctgcgcgcgc agcgctacat
      241 cgccacgttc ttcccgttcg tcgcgttcct gtcggacgtc gcgcaggcgg tcgtgctggg
      301 cgtcggagcg gcccggttgg catcgggtga cctcactccc ggtgtgctca cggcgttcct
      361 gctgtacctc gggctgttct tcgcgcccgt ccagcagctc tcgcaggtct tcgacggcta
      421 ccagcaggcc cgcgtcgggc tctcccgcat ctccgatctc ctgcgtaccc ccacctcggt
      481 gccgccggag cccgcgagcg atcccctgga ggtgccgcac cggctgcgcg gcgaggtcga
      541 gctgcgcgac gtcgggttca cctacgccgg cacgcaggcc cctgctctgg agcacgtgtc
      601 gctgcgggtg gcccccggcg agacgatcgc gctggtcggg gccaccggcg cgggcaagtc
      661 cacagtggtc aagctgctcg cccggttcta cgaagtgggc acgggagccg tgctcgtcga
      721 cggcgtggat gtgcggcgct acccactggg cgactaccgg cgcaggctcg gcatcgtgcc
      781 gcaggagccg cacctgttca cgggcgacgt cgctgccaac atcgcgtacg cccgcccgga
      841 cgccacgccg gccgagatcg aggcggctgc ccgggcggtc ggtgcgctcg acctggtgcg
      901 gggtctgccc ggcgggttcc ggcacccggt cggcgagcgc gggcagggcc tctcggccgg
      961 gcagcggcag ctcgtggcgc tcgcccgcgc cgagctcgcc gatcccgacc tgctgctgtt
     1021 cgacgaggcc accgccgcgc tcgaccccgc caccgaggcc gccgtgctcg cggcgggcga
     1081 ccgggtcatc gcacgccgca cggcgttcgt cgtcgcccac cggctggcca cggctgcccg
     1141 ggccgatcgc atcgtcgtgc tggacgacgg ccggatcgtc gaggtggggc cgcacgccga
     1201 gctgctcgcc gcaggcggtc actacgcgcg gctgtggcgc gcgggggagc tggaacccgc
     1261 ggcgtagccg gatcgcagct cccctgtccg cggcaccaca cccgacagcg tcaccgtccg
     1321 cctcaggccg agctccgtcg gccagcacct gaactcttga cgcgccgggc catgggttcg
     1381 gtaatgcatg ccctccccgg tggaacattc catctcgcca catgctcccc gccggggtgc
     1441 tcggttcctc cgcgacatcc agcaggcgga gccacggcgg cggcgctacg ggcggacgcg
     1501 agctcccgga ccgcggtcgc acgcgacgat ccggcacgtc atgggccccc gatggagcga
     1561 aacgcccccg gcctgcccga tatcgggcag ggaacttggt catccgggcc cgcacagggc
     1621 accatctcct ctcgtgcccg caggcccgta cgacgacctc atcgcgcacc tggtccgtag
     1681 cacgcccctc agcacgggtg aggcggcccg cgtgatcgcg gaggttgtgg gctacttttc
     1741 ggagcagcta ccggatttcg tccggcgccg gcacgcggag atgaaacgtc gcgggttgac
     1801 gaacgagcag atattcacag cactcggcgc agagctggcg acgcggcggg tggcaccacc
     1861 gccattgtcc gaacgccagc tgcggcggat ggtctacggc tgagaggact ctctatgtgc
     1921 ggcatcgtcg gatacgtggg accccaggac gctgctccga tcctgttgga ggggttgggg
     1981 cggttggagt accgcggcta cgacagcgcc ggtctcgccg tcagcgcgaa gggcgggctc
     2041 aaggtgcgca aggtgagcgg ccgcgtggcc gatctcgctg cccagctgcc gccccggttg
     2101 aaagggtcac cgggcatcgg gcacacccgg tgggccacgc acggtgagcc caacgagacc
     2161 aacgcgcacc cgcatctcga cgccgcgggg cggatcgcgg tcgtccacaa cggcatcatc
     2221 gagaacgccg acgaactgcg cgccaagctc gcagccgacg gcgtcgagtt cgtgtcgcag
     2281 accgacacgg aggtcgtcgc gcacctggtg gctgcggcgt tcaccgccgg ggccggtgac
     2341 ctcgagcagg ccgtgcgaca ggcgctgcgt caggtcgtgg gggcgtacgg gctcgcagtg
     2401 ctggacgcgc agcacccgga ccggatcgtc gtcgcccgca acggcagccc ggtgctgctg
     2461 ggcgtggggg agaaggagat gttcgtggcg tccgatgtcg ccgcgctgat cggctacacc
     2521 cgccaggtcg tctacctcga cgacggtgag ctcgcgacga tcacggcggg cggctaccgg
     2581 acctacaccc tcgacgaccg gtccacggcg aagagcccgt ccaccatcga ctgggacgtc
     2641 gtcggcgccg agatcggcaa ccacgcgcac tacctgatca aggagatcca ggagcagccg
     2701 cggacgatcg agcgcgcgct gaagggccgc atcgacgagc gcttcgacac tgcccacctc
     2761 ggcggcctca acctcagcgt gcgcgaggcg cgcgaggtca agcgggtcaa gatcctcggc
     2821 tgcggctcgg cctgctatgc gggggagctg ggagcgcagc tcatcgagga cctcgccagg
     2881 gtgccggcga gcgccgaggc ggcgtccgag ttccgctacc gcaacccggt ggtcgagccg
     2941 gacacgctct acgtcgccgt cagccagtcc ggcgagacca tcgacaccct cgccgcggtg
     3001 caggagctgc agcgcaaggg tgggcgcgtg atcggcatcg tcaacgtcgt gggctcgacg
     3061 atcgcccgcc aggtggacgg cggcatctac ctccacgccg gcccggagat gtcggtggcc
     3121 gcgacgaagt cgttcacctc gacggtcacc gcgttcgcgt tgctcgcgct gcatctcggc
     3181 cgcatccgtg acctgtcacc ggagcagggc cgccgcatca tcgcgggact gaatgcgctg
     3241 ccgggccaga tcaccgagat cctcgagcag tccgacgcga tcgccgaggt cgcggcggag
     3301 atcgcgcaga gccgcagcgt gatgttcgtc ggtcgccgcc gtggctggcc ggtggcccgg
     3361 gagggtgcgc agaagctcaa ggagatctcc tacgtgcatg ccgaggcgta cccctcggcc
     3421 gagctcaagc acggtccgct ggcactgatc agcccggaga tgccgaccgt cgcgatcgtt
     3481 cccgacgacg acctgttgga caagaacatc tcgacgctgt cggagatcaa ggcgcgcaag
     3541 ggccccgtga tcgcgatcgc gcaccgcgag ctgcccgccg acctggccga ccgcacgctg
     3601 gtgatcccga agggcgagcc ggagctcgac ccgatcctgc tgtcgatccc gctccagatc
     3661 ctcgcctacc acgccgccat cgtgctggag cgcgacgtgg acaagccccg caacctcgcc
     3721 aagagcgtca ccgtggagta gttccggcga gccaggggca gagcgctccc ggctcgatgc
     3781 acatcggcac gtgcgggtgg ccgacgtagg tctccaccgg cccgtcggtc cgccagccca
     3841 ggcggtggaa gaaccgctcg ttggccacct gcacgtgcgc gcgcatcagc cgcgcgccgc
     3901 gagcacccgc cgcggccacc gcgaaccgca ccagcggggc gccgatgttc gccgagcgga
     3961 actcgggcag taccgccagc cggtcgccca gccactcgcc aggaccggtg gggaagagtc
     4021 gcaccgcccc ggcggggcgg ccggcgtgaa gaccgagcac gtgcacgacg tcggtgcggg
     4081 cgtcgtgcgc gtcctcgtcg ccttcgggga acagtccctg ctcctcgacg aacacggcgt
     4141 gccggatgcg gtggtgcgcg gcgagcccgg cgacgtcgcg gacggggcgg cagtgcaccg
     4201 ggtcgacgcc gaccagcacg ttgaccggcc gcgccgggcg gatccgccgt cccgatgccg
     4261 atgccgatg
//
LOCUS       DASRSC010000291        15098 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_768608,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000291 DASRSC010000000
VERSION     DASRSC010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 15098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15098
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768608"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1107
                     /locus_tag="VFE39_14350"
     CDS             <1..1107
                     /locus_tag="VFE39_14350"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET6259821.1"
                     /translation="VLAEHPSDPAALVCADVRAGAATDLSALRQFARGCAVVTFDHEQ
                     VPQENLRALVGAGVTVHPHPDALLHAQDKLVMRRRLAGLGAAVPHFAEVRDADDVERF
                     GAEHGWPVVLKAVRGGYDGRGVWMLDSPDPALVAELLGAGTPLMAEEAVPMRRELAAL
                     VARSPHGQAAVWPVVETVQEDGQCVQVLAPAPGLDEAVAVSAQELALRIAAELGVTGL
                     LAVELFERTDGVLVVNELAMRPHNSGHWTIEGSRTSQFEQHLRAVLDYPLGTTTPTAP
                     AVVMANVLGAAHPPAMAMDERLHHLFARFPDVKVHLYGKTERPARKVGHVTVLGADMA
                     DVRQRATLAAEWLSAGVWSDGWSPHTGALEVAHA"
     gene            1109..1612
                     /gene="purE"
                     /locus_tag="VFE39_14355"
     CDS             1109..1612
                     /gene="purE"
                     /locus_tag="VFE39_14355"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005453639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET6259822.1"
                     /translation="MPVPLVGVIMGSDSDWPVMEAAAVALAEFDVPYEVGVYSAHRTP
                     QRMLDYATGAAGRGLRVVIAGAGGAAHLPGMVAAATPLPVIGVPVPLAQLDGLDSLLS
                     IVQMPAGVPVATVAVGGARNAGLLAVRILAAGDPALLARMERFQADLAESVLGKDAAL
                     RAAAAQR"
     gene            complement(2147..2428)
                     /locus_tag="VFE39_14360"
     CDS             complement(2147..2428)
                     /locus_tag="VFE39_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259823.1"
                     /translation="MRKLIVPVVGALALIMGASGGGGGVGDELATPVIAVAATSNCHP
                     SYQPPSDQPCVPDGPDLDCPDIGYQVKVVGPDEYRLDADKDGWGCDSYA"
     gene            complement(2943..3635)
                     /locus_tag="VFE39_14365"
     CDS             complement(2943..3635)
                     /locus_tag="VFE39_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET6259824.1"
                     /translation="MTVAARRGRSPEGRAEVRRDLVAAAVELFRVRGYEDTTVDDIAA
                     AAGVGRRTFFRYFRSKEDAISPDHETALARVAEVFETAHPTEPSAPLVLRAGETVFDI
                     YADDPRLSVDRYRLTHEVPALRDRESASVDHYRRLFTRYLRRRFAGDPDGDLRAAVIG
                     AAVVAAHNLALRAWLAAGAPADGMEACRERFRRVAEMLPGEPAGDDDLHAVTQRLEQA
                     VVRLERTTPPEH"
     gene            3710..4867
                     /locus_tag="VFE39_14370"
     CDS             3710..4867
                     /locus_tag="VFE39_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6259825.1"
                     /translation="MDPDFDTYRLSDEHEALRDAVRALAEKEIAPYAAEVDELGRYPV
                     EARAALVKAGFHAVTIPEEYGGQGADELAGCIVIEEVARVCASSSLIPGVNGLGLTPI
                     LLSASDDLKKQVLPSIAAGEAMISYALSEREAGSDAASMKTRARREGDSWVLNGTKAW
                     ITNAGESTWYTVMAVTDPEKKANGISAFVVHSGDPGFDVGPKERKLGIHGSPTREIYF
                     VDCTIPADRIIGAEGTGFKTALQTLDHTRPTIGAQAVGIAQGALDVAISYVKERKQFG
                     RHLAEFQGLQFMIADMAMKVEAARQLVYVATCRAERGEPNLGFISSAAKCLASDTAMS
                     VTTDAVQLLGGAGYTRDFPVERMMRDAKITQIYEGTNQIQRMVMARALLKA"
     gene            complement(5469..5840)
                     /locus_tag="VFE39_14375"
     CDS             complement(5469..5840)
                     /locus_tag="VFE39_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259826.1"
                     /translation="MSKILLRVAQIVGVLVLAGIAVGVVVGLVQWLLGAAVIVAVPLG
                     AWWLYKRVSSRNPKPTALPGTPKAVTGPSGDRRSEIESRAVLDASGRCGWCGSATLHK
                     DEFGFPTTPLTFHRAEIDAML"
     gene            6062..6457
                     /locus_tag="VFE39_14380"
     CDS             6062..6457
                     /locus_tag="VFE39_14380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016337700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET6259827.1"
                     /translation="MAITLSVGVRALVDGPNFAAVATLDPDGGPQTSVVWVGLDDDTL
                     VFSATEDRRKVRNLRRDPRVSVSITDAANPYRHTQLRGTVTIIADPDKTLPKTLSHKY
                     RGEDPPPEGPEVERVIVRLHVEKVAGNVQ"
     gene            complement(6708..7526)
                     /locus_tag="VFE39_14385"
     CDS             complement(6708..7526)
                     /locus_tag="VFE39_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005443155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 3'-phosphotransferase"
                     /protein_id="HET6259828.1"
                     /translation="MSLSRPVISGIPTGPVAVPDAVKALAGSDSVTPVWRNELGGLTF
                     RLEDGRAATRYAKWVAAGTPELELPGEAERLAWAQRWVTVPRVIEHGTDADGTWLVTE
                     AIPGASAVTPRWIADPATAAAAIGRGLRLLHDALPVNQCPFDWSTDTRLARADERIAD
                     GEGPADWFPDHRHLNLAEARSRIGRPPAIDRLVVCHGDACVPNTLLHDDGTFAAHVDL
                     GSLGLADRWADLAVAAWSAEWNYGRGYDGIVYDAYGIGPDPERIAYYRLLWDMA"
     gene            complement(7568..8290)
                     /locus_tag="VFE39_14390"
     CDS             complement(7568..8290)
                     /locus_tag="VFE39_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03089 family protein"
                     /protein_id="HET6259829.1"
                     /translation="MTLTAALLDPLRSGAAAARPLITYYDDATGERIELSSITAANWA
                     AKTANLLRDECDVEPGTAVAMLLPAHWQTAAVLLGAWWCGAEVAAEPADADWVLCDAD
                     RIDLALAAAPAGGVVALSLDAFGKGLPGLPSGVIDFATEVRLHGDDFVPSAPVPDDTA
                     ALAGATVADVLADARGRAAALGLTASDRVLSTLEWSSPEGLSDGLLAVLAAGASLVHV
                     RHPDPAALDRKATSERTTVRLG"
     gene            complement(8305..9720)
                     /locus_tag="VFE39_14395"
     CDS             complement(8305..9720)
                     /locus_tag="VFE39_14395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET6259830.1"
                     /translation="MVASALVLVLTAAAWGLYRDITAGITTTDVIAGGNDDSADNILL
                     VGVDSRTDAQGNPLPPELQRLLNSGPDSGVLNSDTIILLHVPDGGGAAVAFSLPRDSY
                     VDIPGYRQDKINAAYPATKALAAEQLVADGVRDRQRVEAESAQAGRSTLIRTVENLTG
                     QHIDHYAEINLLGFYNLTTAIGGVDVCLRAPVDEPLSGARFPAGPQTISGAGALAFVR
                     QRHGLPEGDLSRIRRQQAFLATVADKVLSGGTLADPGKLRALIDVAQKSLVIDSGWDV
                     LAFAQQASDIAAGNLEFVTIPTRGLETNTRGDVVLVDPGDVRTFVDQRIEEQQRSAEA
                     AREAPPAPPPQVDVIASRYVVDVRNGSALPGMAATVARRMRDLDFSSGTVDNTDPTST
                     STVHYTGSDAKAARAVAAQLGDLPVESNDEVTPGHLLVVLGGDFNPAVLPAPVDDAPA
                     RPTATPAPSAPITAAGVPCID"
     gene            10240..11067
                     /locus_tag="VFE39_14400"
     CDS             10240..11067
                     /locus_tag="VFE39_14400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6259831.1"
                     /translation="MPEQLSLLLPVWAGDRPDFLTEAFRSSVDGQTRRPDQVVIVRDG
                     PVGASLDARIAELAATSPVPVDVVALERNVGLGPALDAGLAACRHNVVARMDADDISL
                     PHRFAVQMPIIESGVDIVGSGLLEFGAHPDDVVGTRTPPTDPRDIRVRARFADPFNHP
                     TVVYRRDLVRSVGGYTDFALMEDYLLWAKMIVAGARVANVAEPLVKYRVGAGAYARRG
                     GLRQLRAEVSIQRRFRALGFTTRAQFVRNLAVRGGYRLVPERLRRVAYRAVIATYRR"
     gene            11117..11968
                     /locus_tag="VFE39_14405"
     CDS             11117..11968
                     /locus_tag="VFE39_14405"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET6259832.1"
                     /translation="MTQPSSLPPEPLCTQGVALLDSGHALDAVEVLRQAVAAGEPSAP
                     DLLVRAYLDSGSWHAAVEWLTPLVQQGHVRFAGRLGVALAELGDRERAEDTLRLAVES
                     GELAAANDLAILLRDEGRLREAVQLLIQVAEAGDGQAAANAVELHLEAGDLQSAIAAA
                     ERYADENRPDTVVALADVRALQGRTDEAEGFYRRAGELGALRAHTAYGQFLAVARGDI
                     EGAEREFREAERHAEPGWAYTLGRFLLDEGRPEEARPYLQVAVDSGDTAALEALIELD
                     GEDPADD"
     gene            12054..12893
                     /locus_tag="VFE39_14410"
     CDS             12054..12893
                     /locus_tag="VFE39_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-Rha--alpha-D-GlcNAc-pyrophosphate
                     polyprenol alpha-3-L-rhamnosyltransferase"
                     /protein_id="HET6259833.1"
                     /translation="MDRDPIRRDPVQEYGDGLAVVTVTRSVRPDVDRIVGSVRVATTR
                     PVRIVLAAVGQASAVCGDTTGADGMEVLRIGEDIGRAAAVNRAVAGLDAGVGWVAIAD
                     PGVRWGAGVLDGLLATAAGRPRAGLLGPRLRAAAGAVVASAGALPSITQALHGRIPTA
                     ACAGPTGWLSTSCVLVRRSAWDSVDGFDPRYLGAPGPVDVADVDLGDRLDRAGWLLVH
                     VPGLEIDTEIDTVVDTEAGVDAHDGHGILTWRADDLRRYLHDRGRAPVRALLALGGRA
                     GRA"
     gene            12983..14053
                     /locus_tag="VFE39_14415"
     CDS             12983..14053
                     /locus_tag="VFE39_14415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HET6259834.1"
                     /translation="MQDVEAVVLVGGKGTRLRPLTLSAPKPMLPTAGVPFLAHLLSRV
                     RAAGVGRVVLGTSYLAETFSAHFGDGAALGLEIEYVVEDHPMGTGGGIRNVAKYLTTD
                     DVLVFNGDVLCGTDLGAVVDTHRSTDADVTLHLVRVQDPRAYGCVPTDESGSVLEFLE
                     KTEDPPTDQINAGCYVFRRAVIDAIPEGRPVSVERETFPGLLSAGARVSGHVDNAYWR
                     DMGTPADLIHGSADLVRGVAPSAALPGPTGESLVLEGAEIADGALVFGGTTVGRGVRI
                     GAGARVEGAMLFDGAVVGEGAVVEHSVIGAGARVEEGALVNDTVVGDRAVVGAHCELR
                     RGLRIWPDVVLPPHGLRFSPDV"
     gene            14046..15005
                     /locus_tag="VFE39_14420"
     CDS             14046..15005
                     /locus_tag="VFE39_14420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005461698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HET6259835.1"
                     /translation="MSEPAGLAHGHEGGSSRVHAREWRPGYLLDLAGVLAPLRRGRGD
                     PTWRSAGTGSAIWRVSTTPDGAATMRLHRRADGTVVAEAWGPGAQWALDGLPALLGES
                     DRPEEFVAHHPLVADAARRMPGLRFGASGRVWDVLVPSVLEQKVTGVEARRSWRELCW
                     RFGDPAPGPAPDGMRVPPTPVQIRAVPDWEWHRAGVDSARRRAILACAAVAHRLETAC
                     ELGGERGRELLRKVPGIGVWTAAEVAQRAWGDPDAVSVGDFHIPTLVGWALVGRPLDD
                     TAMLAVLAPYSPQRQRAVRYVEVSGFRRPRFGPRYSPKDYRAM"
BASE COUNT         2022 a         5250 c         5647 g         2179 t
ORIGIN      
        1 gtgctcgcgg agcacccctc cgacccggcc gcgctggtct gcgccgacgt gcgtgccggg
       61 gcggccaccg acctgtcggc gctccgccag ttcgcgcgcg ggtgcgccgt ggtcaccttc
      121 gaccacgagc aggtgccgca ggagaacctg cgcgcgctcg tcggcgcggg agtgaccgtc
      181 cacccgcacc cggacgcgct gctgcacgcg caggacaagc tggtgatgcg gcgcaggctt
      241 gctgggctcg gcgccgccgt gccgcatttc gccgaggtgc gcgacgccga cgacgtcgag
      301 cgcttcggcg ccgagcacgg gtggcccgtg gtgctcaagg ccgtccgcgg cgggtacgac
      361 gggcgcggcg tctggatgct cgactctccg gatccagcgc tggtcgccga gctgctgggg
      421 gccggcacgc cgctgatggc cgaggaggcc gtgccgatgc ggcgggagct ggccgcgctc
      481 gtcgcgcggt cgccgcacgg gcaggcggcg gtgtggccgg tggtggagac ggtgcaggag
      541 gacggccagt gcgtgcaggt gctggcgccc gcacccggcc tcgacgaggc cgtggccgtc
      601 tcggcgcagg agctcgccct gcgcatcgcg gccgagctgg gcgtcacggg cctgctggcc
      661 gtggagctgt tcgagcgcac cgacggggtc ctggtcgtca acgagctcgc catgcgcccg
      721 cacaactccg ggcactggac gatcgagggc tcgcgcacat cgcagttcga gcagcacctg
      781 cgggcggtgc tcgactaccc cctcggcacg acgacgccca ccgctcccgc cgtggtgatg
      841 gccaatgtgc tgggagccgc gcatccgccc gcgatggcca tggacgaacg gctgcaccat
      901 ctcttcgccc ggttccccga cgtcaaggtg cacctgtacg gcaagacgga acggcccgcc
      961 cgcaaggtcg ggcacgtgac ggtgctcggc gctgacatgg ctgatgtgcg ccagcgcgcc
     1021 acactcgccg cggagtggct ctcggcgggc gtgtggtccg atggctggtc cccgcacacg
     1081 ggtgcgttgg aggtggcgca tgcctgacgt gcccgtcccg ctcgtcgggg tgatcatggg
     1141 tagcgactcg gactggccgg tgatggaggc cgctgccgtt gctctcgccg aattcgacgt
     1201 gccctacgag gtgggcgtgt actcggcgca ccgcaccccg cagcgcatgc tcgactacgc
     1261 gacaggcgcg gctgggcgcg ggctgcgggt ggtgatcgcc ggcgccggtg gtgctgcgca
     1321 cctgcccggg atggtcgccg cggccacacc gttaccggtg atcggggtgc cggtgccact
     1381 cgcccagctc gacgggctgg actcgctgct gtcgatcgtg cagatgcccg ccggggttcc
     1441 ggtggccacc gtggcggtgg gcggcgcgcg caacgcgggc ctgctggccg tccggatcct
     1501 ggcggccgga gacccggcgc tgctggcccg gatggagcgg ttccaggccg acctggcgga
     1561 gtccgtcctc ggcaaggacg cggcgctccg cgctgccgcg gcgcagcgct gaggatcacc
     1621 gaaaatcgcg tggcgcgctg ctgctctccg gtcgtagtgt ggcggtacac gtgagaggag
     1681 gtggtccagc gttgattagc tacaggactc gtgaggtggc tgtccgctag cagcacagga
     1741 cacgtttctc cgacggccaa gtggaagccg gtgtggtgcc ggcgaattcc aggcagtcac
     1801 ccgtgatccc cgggcccgag cccgtccagc tcggctgcgc accccaacgg tgcggcccgg
     1861 ggatcacgca cgtccagcct cccacgagag cccgacatgg acacgtatcg ctgatcggga
     1921 cggcgcgcga acagcgacag gtgtccgtgt cgagagcctc accggaccta ccgggttttc
     1981 gccggtagac aagggccagc gcgggggcgg taagcccggc gttgtcctcc agtgcgcggg
     2041 cgagcccgcg ggcggcgagg gtggtgcacc acaccgtgcc gcgccgcacg agggagcgga
     2101 gcgttgtcct tgctccaggc ccctcggcga ccgaaagcgg gagcggctag gcgtagctat
     2161 cgcagcccca cccgtccttg tcggcgtcga gccggtactc gtccggcccg acaaccttca
     2221 cctggtaccc gatgtcgggg cagtccaggt cgggaccgtc agggacgcac ggctgatcgc
     2281 tcggcggctg atagctcggg tggcagttcg acgtggccgc gacagcgatg accggagtgg
     2341 cgagctcgtc gccgacaccc ccaccgccac ccgacgcgcc catgatcagg gccaacgctc
     2401 ccacgacggg aacgatcagc ttgcgcacgt ttatcccttt cctcgctgga tgcgtggtgc
     2461 atccgcacac tgatcgttgg cctttcaact tccgttactg atctaggttg cagcgggtgg
     2521 acaacggccg cgcaacgtcc actgtggaac gaatccggta tgtgccgcgc ctgcctgtgc
     2581 tgtcggccat ctcgctcgcc gagctgcgca ccctccccgc gctcgccgcc gccgcctgcc
     2641 gcgcactcgg ccgctctgct ctgggacgac ggactccccc acgaggccga cgacgaccgc
     2701 gacagccgtc accgcctcgc cgccaaggtg cgccacggct gcccagcccg ccacgactgc
     2761 gcccacctcg ccaccagcta gggggaccgg atcgaggacg tgcgggccgg tcgactcaac
     2821 cccccgcgac ccgagcgcgg cgcgcgcgac gggcggctgt cgcaggtaga tggcctgtcc
     2881 gtgtcaatca gcacggcaag cggcgttctc ccggcatgtg tccatgtcgc gcagcgggag
     2941 ggtcagtgtt cgggcggcgt ggtgcgttcc aggcggacca ccgcctgctc gaggcgctgt
     3001 gtcaccgcgt gcaggtcgtc gtcgcccgcg ggctccccgg ggagcatctc cgccacccgc
     3061 cggaaccgct cgcggcacgc ctccatcccg tccgcgggag ccccggcagc gagccacgcg
     3121 cgcagcgcca ggttatgcgc agccaccacg gccgccccga tgacggctgc gcgcaggtcg
     3181 ccgtccggat cgccggcgaa ccgccggcgc aggtagcggg tgaacagtcg gcggtagtgg
     3241 tccacgctcg ccgactcgcg gtcgcgcaga gccggcacct cgtgggtgag ccggtagcga
     3301 tccaccgaca gccgcgggtc gtcggcatag atgtcgaaca cggtctcgcc ggcacgcagc
     3361 acgagcggtg cggagggctc cgtggggtgc gcggtctcga agacctcggc gacgcgggcc
     3421 agcgcagtct cgtggtcggg cgagatcgcg tcctccttgc tgcggaagta ccggaagaag
     3481 gtgcggcgcc cgacgcctgc cgcagcggcg atgtcgtcga ccgtcgtgtc ctcgtagcca
     3541 cggacccgga acagctcgac ggcggcggcc acgaggtcgc gccggacctc cgcgcgtccc
     3601 tcgggcgagc ggccgcggcg ggcggcgact gtcatctggc ctccttcaat ggcacacagg
     3661 gtagcaaaga gcttgtacag tggcacgcag tgccgttacg ctgccacgca tggatcccga
     3721 cttcgacacc tatcggctca gcgacgagca cgaggcgctg cgggacgcgg tgcgcgcgct
     3781 cgcggagaag gagatcgcgc cgtacgcggc cgaggtggac gagctgggcc gctacccggt
     3841 ggaggcgcgg gccgcactgg tcaaggccgg gttccacgcc gtgacgattc ccgaggagta
     3901 cggcggccag ggggccgacg agctcgccgg ttgcatcgtg atcgaggagg tcgcgcgcgt
     3961 ctgcgcgtcg tcgtcgctga tcccgggcgt caacgggctg ggcctgaccc cgatcctgct
     4021 ctcggcgtcc gacgacctca agaagcaggt gctgccctcc atcgccgcag gtgaggcgat
     4081 gatcagctac gcgctctcgg agcgggaggc gggctccgac gccgcgtcga tgaagacccg
     4141 cgcccgccgc gagggtgact cgtgggtgct gaacggcacc aaggcctgga tcaccaacgc
     4201 gggggagtcc acctggtaca ccgtcatggc ggtgaccgac ccggagaaga aggcgaacgg
     4261 catctccgcg ttcgtcgtgc actccggcga cccgggcttc gacgtcggtc cgaaggagcg
     4321 caagctcggc atccacggct cgccgacgcg cgagatctac ttcgtcgact gcacgatccc
     4381 ggccgaccgc atcatcgggg ccgagggcac cggcttcaag acagcgctgc agacgctcga
     4441 ccacacccgc ccgaccatcg gcgcacaggc cgtgggcatc gcgcagggcg ccctcgacgt
     4501 cgcgatctcc tacgtcaagg agcgcaagca gttcggtcgc cacctcgccg agttccaggg
     4561 cctccagttc atgatcgccg acatggcgat gaaggtggag gccgcccgtc agctcgtcta
     4621 cgtcgccacg tgccgtgcgg agcgcggcga gccgaacctc ggcttcatct cgagcgcggc
     4681 gaagtgcctg gcatccgata cggcgatgag cgtgacgacc gacgcggttc aactgctcgg
     4741 tggtgccggg tacacgaggg acttccccgt ggagcgcatg atgcgcgacg cgaagatcac
     4801 ccagatctac gagggcacca accagatcca gcgcatggtg atggcccgcg ccctgctcaa
     4861 agcctagatt gccgatcttg gcccacggcc gtccttcgcc ggctttcatg atcaggccgc
     4921 cgtcacgccg tcagtgctct cgccggaggc gagggtgaag acaccgtcga tgaccgtccg
     4981 ggcacgttgg tagctggacg gcacgaggtg ggtggaagtg tgagggtgaa gcctggatcg
     5041 gcatgcccga ggtagtcggc aagctcctcg atcgagacgc tcagcccccg gggaggggac
     5101 gaccatcgtc gtcgagctac cgctgccccg cagctgcgcg agcacgtcga ggcgttcggg
     5161 cggcccagag gtgtcggcgt agtcgtcgat ccgcgccgac cggcgggccg gaagacgagc
     5221 ccggccctgt tctgcccgcc gaggctcgcc cgggcgccgg ggacgggcat cgctgtagag
     5281 cccggcggcg ccgccatcgc ggtcgtgact gaacgcggcc ggcttgccga cagaccgagt
     5341 ccggctggaa ccccgcgcga gcggtgtgca cggccgccgc gcggttacga cgttgagcct
     5401 gcgctcggcg gagcgcggga gacagcctgc gctctcagcc gtggggagca tttcctgccg
     5461 gcccggggtc agagcattgc gtcgatctcg gcgcggtgga aggtcagcgg cgtcgtgggg
     5521 aagccgaact cgtccttgtg cagggttgcc gatccgcacc agccgcagcg gcccgacgcg
     5581 tccagtacgg cgcggctctc aatttcgcta cggcggtcac cggagggacc ggtcacggcc
     5641 ttcggcgtgc ccggcagtgc tgtgggcttc gggttgcggc tggaaacccg tttgtagagc
     5701 caccaggcgc cgagcgggac cgcgacgatc actgcggcgc cgagcagcca ctgtacgagc
     5761 ccgaccacga cgcccaccgc gatgcccgcc agcacgagta ccccgacgat ttgcgccact
     5821 cgcagcagga tcttcgacat agtcggtgtc ccttcgcgtg cccggattcc ccgccgcctc
     5881 tggccgacgg taccgggaca catctggatg gggtggcggc cgttcagcgc tgctcgatcg
     5941 cccatccggt cggtgttagg ccgcacaacc ggctgacact tttccccgca tcgcccaccg
     6001 ctgaacggcc tggtcagagc gtgcgggaat agcgtccggg ccggcgcaag ttcggcctga
     6061 catggcgatc actctctcgg tcggcgtgcg cgccctggtc gacggcccga acttcgcggc
     6121 agtggcgacg ctcgacccgg acggaggtcc gcagacgtcg gtcgtctggg ttggcctcga
     6181 tgacgacacc ctcgtgttca gcgcaaccga ggaccggcgg aaggttcgca accttcggcg
     6241 ggaccctcgc gtgagcgtgt cgatcactga tgcggcgaac ccgtaccggc acacccagct
     6301 gcgcggcacg gtgacgatca tcgcggaccc ggacaagacg ctgccgaaga cgctgagcca
     6361 caagtaccgc ggcgaggacc cgcccccgga ggggccggag gtcgagcggg tgatcgttcg
     6421 gctgcacgtc gagaaggtcg ccggcaacgt ccaatgatcg actttcaggc aatctgatgg
     6481 agatgacacg agcgtcagca gcctgagggt caacggcagc accgggtgca gacagatcat
     6541 ctgttggggt ggcatttgat cagctgtgat ccatggtccg gcttgcaggt cagctcgtcc
     6601 gcctggtcct ctctgtcggt aagaaggcta tcgctcagcc tttgcttcac gggtcgtcga
     6661 tcaccacgag ccaggaaccg tagatccccg agattgatgc gagccggtca agccatgtcc
     6721 cagagcaggc ggtagtaggc gatccgctcc gggtccgggc cgattccgta cgcgtcgtag
     6781 acgatgccgt cgtatccacg gccgtagttc cactccgcgc tccaggcggc gacggcgagg
     6841 tcggcccacc ggtcggccag cccgagcgaa ccgaggtcga cgtgggcagc gaacgtgccg
     6901 tcgtcgtgaa gcagtgtgtt ggggacgcac gcgtccccgt ggcacacgac gaggcggtcg
     6961 atcgcgggtg gccggccgat gcgggaccgg gcctccgcga ggttgaggtg ccgatggtcg
     7021 gggaaccagt cggccggtcc ctcgccgtcg gcgatgcgct cgtcggcgcg tgcgagccgg
     7081 gtgtcggtgc tccagtcgaa cgggcactgg ttcacaggta gggcgtcgtg gagcaggcgg
     7141 agccctcggc cgatcgctgc cgcagccgtc gccgggtccg cgatccaccg aggggttacc
     7201 gccgaggcac cagggatcgc ttccgtgacc agccatgtgc cgtcggcgtc cgtaccgtgc
     7261 tcgatgacgc gcgggacggt cacccaccgc tgcgcccagg caaggcgctc cgcctcgccg
     7321 gggagctcga gttccggggt gccggcggcg acccacttgg cgtacctggt tgcggcgcgc
     7381 ccgtcctcga ggcggaacgt gagaccgccg agctcgttgc gccacaccgg agtgacggag
     7441 tcgctgccgg cgagcgcctt gaccgcgtcg ggaaccgcca cgggaccggt cgggatcccc
     7501 gagatgacgg ggcggctcag gctcatgatc ctcccccctg cactgcagcc cccgacggcg
     7561 gccgggatca gcccaggcgc accgtcgtgc gttccgaggt cgccttccgg tccagcgcgg
     7621 ccgggtcggg gtggcggacg tgcaccagtg acgccccggc ggccagcacc gcgagcaggc
     7681 cgtcgctcag gccctctggg ctcgaccact ccagcgtgga gagcacgcgg tcggacgccg
     7741 tgagcccgag tgcggcggcc cggccgcggg cgtcggcgag cacgtcggcc acggtggccc
     7801 cggccagggc cgcggtgtcg tcggggacgg gggccgacgg tacgaagtcg tcgccgtgca
     7861 gccgcacctc ggttgcgaag tcgatcacgc ccgacggcag cccggggagc ccctttccaa
     7921 acgcgtcgag cgacagcgcc accaccccgc ccgccggggc tgcggcgagt gcgaggtcga
     7981 tgcggtcggc gtcgcacagc acccagtccg catcggcggg ctcggccgcg acctcggccc
     8041 cgcaccacca cgcgcccaac agcaccgctg ccgtctgcca gtgcgcgggc agcaacatgg
     8101 ccaccgcggt accgggttcc acatcgcact cgtcgcggag caggttcgcg gtcttcgccg
     8161 cccagttggc ggcggtgata ctggacagct cgatgcgctc acccgtcgcg tcgtcgtagt
     8221 aggtgatcag cggtcgggcg gcggccgcgc cggagcggag cgggtccagc agggctgcgg
     8281 tgagcgtcac gggctcaccg taggtcagtc gatgcaggga acgcccgccg ccgtgatcgg
     8341 cgccgacggc gcgggggtgg cggtcgggcg ggccggggcg tcgtcgacgg gagcgggcag
     8401 gaccgccgga ttgaagtcgc cgccgagtac cacgagcagg tgaccggggg tcacctcgtc
     8461 gttgctctcg accgggaggt cccccagttg ggcagccacc gcacgcgcgg ccttggcgtc
     8521 ggagccggtg tagtggaccg tcgaggtcga tgtggggtcg gtgttgtcca ccgtgcccga
     8581 ggagaagtcg aggtcacgca tgcggcgggc cacggtggcg gccatcccgg gcagggcgga
     8641 gccgttgcgt acgtcgacga catagcgcga cgcgatcacg tcgacctgcg gcggaggcgc
     8701 gggcggcgcc tcgcgggcgg cctctgcact ccgttgttgc tcctcgatcc gctggtcgac
     8761 gaacgtgcgc acgtcacccg gatcgaccag cacgacgtcg ccgcgtgtgt tggtctccag
     8821 gccgcgggtg gggatcgtga cgaactccag gttgcccgcc gcgatgtcgg acgcctgctg
     8881 cgcgaacgcg aggacgtccc agccggagtc gatgaccagc gacttctggg cgacgtcgat
     8941 caacgcgcgg agtttgcccg gatcggccaa agtgccgccg gagaggacct tgtccgcgac
     9001 cgtggcgagg aacgcctgct gtcggcggat ccgcgagagg tcgccttcgg gcagtccgtg
     9061 ccgctgccgg acgaacgcga gcgcgccggc gccggagatc gtctgcggcc cggcggggaa
     9121 ccgggcgccc gacagcggct cgtcgaccgg ggcccgcagg cagacgtcga cgcccccgat
     9181 cgccgtcgtg aggttgtaga agccgagcag gttgatctcg gcgtagtggt cgatgtgctg
     9241 gcccgtgagg ttctcgacgg tgcggatcag cgtgctgcgg cccgcctgcg ccgactcggc
     9301 ctccacccgc tgccggtcgc gcaccccgtc ggccacgagt tgctcggccg ccagtgcctt
     9361 cgtcgcggga taggcggcat tgatcttgtc ctggcggtaa ccggggatgt cgacgtagct
     9421 gtcgcgggga agggagaagg cgaccgcggc gcccccgccg tcggggacgt ggagcaggat
     9481 gatcgtgtcc gagttgagca caccggagtc ggggccgctg ttcaggaggc gctgcaactc
     9541 gggcggtagc gggttgccct gcgcgtccgt gcggctgtcc acgccgacca gcaggatgtt
     9601 gtccgcgctg tcgtcgttcc cgcccgcaat gacgtcggtg gtggtgatcc cggcggtgat
     9661 gtcgcggtag agaccccaag cggcagcggt gagcaccagg acgagcgccg acgccaccac
     9721 gagggcgatc cggagccgct gccggagggg gagccgggct ttcgccgcag gcgcgggcga
     9781 cgcaggccgg gcgcgtccgg gccggacctt cgtcgtaccc ggtggcgctg cagctcggct
     9841 cgtggtgcgt gagccggacg tccgggaccc acctcgcggc ctcccggccg acgtcccggt
     9901 cgtcgacaac ggatcgggcg tcgtccggcg ggcggacggg taccgggccg atctttccga
     9961 ggaaacctcc ggctcccgcc ggcctgatcc gccggagcgg tacgggctgc tgcgctcccg
    10021 attggacggt gaccgggccg agccgtcggt ccagccccgt ccgttcacgt gtgcacctcc
    10081 gctgaccgtc gttgcgcggt ctcctagggt gcagcaccgg cgcgcccggc cgggcgcgcc
    10141 ggtgcgcgtg tcgcgggtga tcgtcaacgg tgatgatcgt gcgttcgtca cacatggtgc
    10201 ttccgggctc gcctgcccgg gaccagaggg gagccatcgt tgcccgagca gctgtcgctg
    10261 ttgctgccgg tctgggccgg tgatcggccc gatttcctca cggaggcgtt ccgctcctcg
    10321 gtggacggcc agacccggag gccggaccag gtcgtgatcg tgcgtgacgg tccggtgggc
    10381 gcgtcgctcg acgcgcggat cgcggagctc gccgcgacga gcccggtacc ggtggacgtg
    10441 gttgcgctgg aacgcaacgt cggcctcggc cccgcgctcg acgccgggct cgcggcctgc
    10501 cgtcacaatg tcgtcgcgcg gatggatgcc gacgacatct cccttccgca ccggttcgcc
    10561 gtacagatgc cgatcatcga gtcgggcgtg gacatcgtgg gatccggctt gctggagttc
    10621 ggcgcgcatc ccgacgacgt cgtcggtacc cggacgccgc cgaccgaccc gcgcgacatc
    10681 cgcgtccggg cgcggttcgc cgacccgttc aaccacccga cggtcgtgta ccggcgcgat
    10741 ctggtgcgat ccgtcggtgg ctacaccgac ttcgccctca tggaggacta cctgctctgg
    10801 gccaagatga tcgtcgcagg agcgcgggtg gcgaacgtcg cggagccact cgtgaagtac
    10861 cgcgtcgggg caggcgccta cgcgcggcgt ggcgggctgc gccagcttcg cgcggaggtc
    10921 tccatccagc gccgcttccg ggcgttgggg ttcacgacac gggcccagtt cgtgcgcaac
    10981 ctcgcggtac gagggggcta ccggctggtc cccgagcggc tgcggcgggt ggcctatcgc
    11041 gcggtcattg ccacctaccg acggtgaccg ggcgcgggcg ggatgggagc atcactcacc
    11101 cgatcgaggg agtccgatga cccagccgtc ctccttgccg cccgagcccc tgtgcacgca
    11161 gggcgtcgcc ctgctcgact ccggccacgc cctcgacgcg gttgaggtgc tgcgtcaggc
    11221 ggtggccgcg ggtgagccgt cggcccccga cctgctggtg cgcgcctacc tcgacagcgg
    11281 gagctggcac gccgccgtcg agtggctgac gccgctcgtg cagcagggcc atgttcgctt
    11341 cgccggcaga ctcggggtgg cgctcgccga gctcggcgac cgggagcggg ccgaggacac
    11401 gctgcggctg gccgtcgagt cgggtgagct cgcggcggcc aacgacctcg cgatcctgct
    11461 ccgcgacgag ggcaggctcc gcgaggcggt gcagctgctc atccaggtcg cggaggccgg
    11521 tgacggccag gccgccgcga acgcggtcga gctgcacctc gaggccggag acctgcagtc
    11581 cgccatcgcc gcggccgagc ggtacgccga cgagaaccgc cccgacaccg tcgtcgccct
    11641 cgccgacgtc cgggcgctcc agggccgcac cgatgaggcc gaggggttct accggcgggc
    11701 aggcgagctg ggagccttgc gcgcccacac cgcatacggc cagttcctgg cggtcgcccg
    11761 gggcgacatc gagggtgccg agcgcgagtt ccgggaggcg gagcgccacg ccgagcccgg
    11821 ctgggcgtac acgctcggcc gcttcctgct cgacgagggc aggcccgagg aggcgcggcc
    11881 ctacctgcag gtcgcggtcg actccgggga cacggcggcg ctggaggcgc tgatcgagct
    11941 ggacggcgag gacccggctg acgactgaac gcggaacatg cgggagcttc gccgcgccgc
    12001 accgcgggtg gccgccgtgc ccccgcgctc ccgcgccgtt agggtggcgc gccgtggacc
    12061 gtgacccgat ccgccgcgac cccgtgcagg agtacggcga cgggctcgcc gtggtcacgg
    12121 tgacgcgctc ggtccggccc gacgtcgacc gcatagtcgg ctcggtgcgc gtggcgacga
    12181 cccgcccggt ccgcatcgtg ctggccgccg tcgggcaggc gagcgcggtg tgcggcgaca
    12241 ccaccggtgc ggacgggatg gaggtactgc gcatcggcga ggacatcggg cgtgccgccg
    12301 ccgtcaatcg cgccgtggcc ggtctggacg cgggggtggg ctgggtcgcg atcgccgacc
    12361 ccggggtgcg gtggggggcc ggggtgctgg acggactcct cgcgactgcg gccgggcgcc
    12421 cgcgcgccgg cctgctcggc ccccggctgc gcgccgcggc aggcgccgtc gtggcgtccg
    12481 ccggggcgct gccctcgatc acccaggcgc tgcatggccg gatccccacc gcagcctgcg
    12541 ccggcccgac gggctggctg tcgacgtcgt gtgtgctggt gcggcgcagc gcctgggact
    12601 cggtggacgg gttcgacccc cggtacctcg gcgcccccgg tccggttgac gtggccgacg
    12661 tcgacctcgg cgacaggctg gaccgggccg gctggttgct cgtccacgtg ccgggcctcg
    12721 agattgacac ggagatcgac accgtcgtcg ataccgaggc cggtgtcgac gcgcatgacg
    12781 ggcacggcat cctgacgtgg cgcgcggacg atctgcgccg ctatctgcac gaccgcggac
    12841 gcgcacctgt ccgggcgctg ctggcgctcg ggggtcgtgc aggccgagcg tgaccgagtg
    12901 gaaaaccgcg tgcaggaaca gcgagtaccg aacaccgtaa gccgctgacc gagctgtaac
    12961 ccgagctgga ggaggacgcg tgttgcagga cgtcgaggcc gtggtgctgg tcgggggcaa
    13021 ggggacgcga ctgcgtccgc tcacgctgtc ggccccgaag cccatgctgc ccacggcggg
    13081 cgtgccgttc ctggcgcacc tgctgtcccg ggtccgtgct gcgggtgtcg ggcgcgtcgt
    13141 gctcggcacg tcgtacctcg ccgagacgtt ctcggcgcac ttcggcgacg gtgccgccct
    13201 cgggctcgag atcgagtacg tggtggagga ccaccccatg ggcaccgggg gtggtatccg
    13261 caacgtcgcc aagtacctca ccaccgacga cgtcctcgtg ttcaacggcg acgtgctgtg
    13321 cggcaccgac ctgggtgccg tcgtcgacac gcaccgaagc accgacgccg acgtgacgct
    13381 gcacctggtg cgcgtgcagg acccccgtgc ctacggctgc gtccccaccg acgagtcggg
    13441 ttcggtgctc gagttcctgg agaagaccga ggacccgccc accgaccaga tcaacgcggg
    13501 ctgctacgtc ttccgccggg cggtgatcga cgcgatcccc gagggacgtc cggtatcggt
    13561 ggagcgggag acgttccccg gcctgctctc ggcgggggcg cgggtctcgg ggcatgtcga
    13621 caacgcctac tggcgcgaca tgggcacccc ggccgacctg atccacggtt cggccgacct
    13681 ggtgcgcggg gtcgccccct ccgctgcgct gcccggaccc acgggcgagt cgctggtgct
    13741 cgagggcgcc gagattgccg acggcgcgct ggtgttcggc ggcacaacgg tgggtcgggg
    13801 cgtccggatc ggggcgggtg cccgggtgga gggcgccatg ctgttcgacg gtgccgtggt
    13861 cggtgagggc gcggtggtgg agcactccgt gatcggcgcg ggcgcccggg tcgaggaggg
    13921 tgcgctggtc aacgacacgg tggtggggga ccgcgcggtc gtcggggcgc actgcgagct
    13981 gcggcgcggc ctccggatct ggccggacgt ggtcctgccc ccgcacggac tgcgcttctc
    14041 cccggatgtc tgagcccgcc gggctcgcgc acggacacga gggcggaagc tcccgtgttc
    14101 acgcccggga gtggcggccg gggtacctgc tcgacctcgc cggcgtgctc gccccgctgc
    14161 gccggggccg gggcgatccg acgtggcgct ccgcgggcac gggatcggcg atctggcggg
    14221 tctcgaccac cccggacggt gcagcgacga tgcggctgca ccgccgcgcc gacggcaccg
    14281 tcgtagccga ggcgtggggg cccggcgcgc agtgggcgct cgacgggctg ccggcgttgc
    14341 tcggcgagtc tgaccgcccc gaggagttcg tcgcgcacca cccgctggtc gccgacgcgg
    14401 ctcggcgcat gccgggcctg cgcttcggcg catccggtcg cgtctgggac gtactggtgc
    14461 cctcggtgct ggagcagaag gtgaccggcg tggaggcgcg ccggtcgtgg cgggagctct
    14521 gctggcggtt cggggacccc gcccccggac ccgcgccaga cggcatgcgc gtgcccccga
    14581 cgcccgtaca gatccgggcc gtgcctgact gggaatggca ccgcgcgggc gtcgactccg
    14641 cccggcggcg cgcgatcctc gcctgcgccg cggtggcgca ccgtctcgag acggcgtgcg
    14701 agctgggtgg ggagcgaggg cgcgagctgc tgcgcaaggt ccccgggatc ggtgtgtgga
    14761 ccgcggccga ggtggcgcag cgggcgtggg gcgaccccga cgcggtgagc gtcggcgact
    14821 tccacattcc cacgctggtg ggctgggccc tggtcggccg cccgctggac gacaccgcga
    14881 tgctcgcggt gctggccccg tactcgccac agcgccagcg cgcggtgcgg tacgtggagg
    14941 tatccgggtt ccgtcgtccc cgattcgggc cgcggtactc cccgaaggac taccgcgcga
    15001 tgtgagggcc ggacgcggat gtaactctca gctccctgcg gtggcccggg gggagcgcgg
    15061 accacgagct ccgtggccag ctcgacgtgg tgggagtt
//
LOCUS       DASRSC010000292        32646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_607334,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000292 DASRSC010000000
VERSION     DASRSC010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 32646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32646
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_607334"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..967
                     /locus_tag="VFE39_14425"
     CDS             203..967
                     /locus_tag="VFE39_14425"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET6259836.1"
                     /translation="MPGFIQSIERAAAVLRLLGTTDRPLGLKEIAAALDLPAPTAHGI
                     VRTLREVGFVEQDMETSRYRLGPGLRELRSGGWDHHDLRARAMNWADSLAGSTGLAVH
                     LGVAVTRSVRLVHHVFRPDGSPQRIRTGEEQPLHATALGKCLLAFAPVATPHARELDL
                     QRWTGRTCTTVPALEAHLAVARRRGWASDTGEYEAGVGGAAAPLRASGGIVVAAIGVV
                     GPVEELFGLGGELRPWLLEQMVGAAREISAALVEGQ"
     gene            1023..2534
                     /gene="glpK"
                     /locus_tag="VFE39_14430"
     CDS             1023..2534
                     /gene="glpK"
                     /locus_tag="VFE39_14430"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET6259837.1"
                     /translation="MTERVVAAIDQGTTSTRCLLFNRSGRMLAVAQREHRQHYPRPGL
                     VEHDAAEIWRNVTRVVPAALRSAGLTPDNIVGLGIANQRETTVIWNQHTGVPLARAIT
                     WQDTRTDGIVARLAEDGHAPLIKEICGLPPATYFAGPRLRWLLDHVPGAREGAEAGEV
                     LFGTMESWLIWWLTGGPDGGRHVSDVTNASRTMLMDVRTLEWSDELLDVLAVPRRMLP
                     EIRPNAEVYGTCTAVLPGVPVAGALGDQQAALVGQACFSPGEIKCTYGTGAFLLMNTG
                     PRLAESTHGLIPTVGYLLGDKPVYALEGAIAMTGSLVQWFRDSLGMIGTAAQIETLAS
                     SVPDNGGCYIVPAFSGLYSPRWHPDARGVMVGLTSFINRGHLARAVLEATGWQTREVI
                     DAMNADSGVPVTRLKVDGGMTANHLLMQFVADVLDVPVERPLVSEAVSLGAAYAAGLA
                     VGHWSDLEVLRSNWHRAAAWEPAMDAVLRRREHENWGRAVSRSYDWVQTPAGD"
     gene            complement(2547..4016)
                     /locus_tag="VFE39_14435"
     CDS             complement(2547..4016)
                     /locus_tag="VFE39_14435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020634469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HET6259838.1"
                     /translation="MTNSLEHAGAAREAAAHDTPEYDPRLTNEDLAPLREQRWTSYNF
                     FAFWMSDVHSVGGYVTAGSLFALGLAAWQVLVALLIGILIVYLLCNLVARPSQATGTP
                     YPVICRASFGVLGANVPAIIRGLIAVAWYGIQTYLASHSLVIVALTLWPRLVPYADAQ
                     QYGFAGLSLLGWIAFVAMWVLQAAVFWNGMEAIRKFIDFCGPAVYVVMFLLAGYLVMA
                     AGWSNIDLNLAEVSITGWAAVPVMLTAIALVVSYFSGPMLNYGDFARYGRSFAAVRRG
                     NLLGLPLNFLVFALLVVVTAAATQPVFGELITDPVETVARLDNAYAVVLGALTFMIAT
                     IGINIVANFVSPAFDFSNVNPQKISWRAGGMIAAVGSVLITPWNLYSSPEVIHYTLDT
                     LGAFIGPLYGVLIADYYLIKKRRIVVDDLYTLRSDGSYHFHRGYNPTAIGATAVGAVL
                     AMVVVLFGSSEAAAYSWFIGAGIAFLLHTVVSRRRTRTA"
     gene            complement(4126..4860)
                     /locus_tag="VFE39_14440"
     CDS             complement(4126..4860)
                     /locus_tag="VFE39_14440"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HET6259839.1"
                     /translation="MRIMVVNPNTTATITDKIGACAQAAASPGTMVLAVSPDMGPVSI
                     ESHYDEALAVPGLLEQIARGEADGVDGYVVACFGDPGLDAARELAAGPVVGIAEAAMR
                     TAAYLGRGFSVVTTLGRTCGRAWDLAERYGVSRFCRNVRACEIPVLELDAPGSDAREV
                     ITEECRRAVAQDGSDVIVLGCAGMADLCADISARIGVPVVDGVAAATRTAESLVALGL
                     RTGKRGEFAAPRPKSYVGLLGGFTIT"
     gene            complement(4883..5560)
                     /locus_tag="VFE39_14445"
     CDS             complement(4883..5560)
                     /locus_tag="VFE39_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HET6259840.1"
                     /translation="MRTVLLDLDGTLVDSAITITEHLAAALTEVGFPVPDAERLRRLV
                     GPPFETALPQLGLTDEQTSAVITAYRASYDAVAATVTPVYPGVPALLERLREADLRLA
                     IATSKPEQLARKIVAGVGLAGYIELVGGADHVAGRVGKAAVVGSVIERLGVDPAREPV
                     VMVGDRHHDVDGAAEHGIPTIGVAWGYAEPGELDSARLVVTDVQKLAEALRGDVVWST
                     VPPAQRS"
     gene            5709..6839
                     /locus_tag="VFE39_14450"
     CDS             5709..6839
                     /locus_tag="VFE39_14450"
                     /inference="COORDINATES: protein motif:HMM:NF021466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HET6259841.1"
                     /translation="MTGPDWGKLIIAVGLRVEDAAESVIAALARLRGRRHPVIVPFIG
                     HGTTRRARVGARLVLGRDAAAPSVLASAAGGDAPSQRSRRAVLRASIARFLTIEVPHA
                     EVTIHLPGGDVVVHTDRDGYVDHEVELPDVAPGWLEVGLTGPAGSSATARVLVADPEA
                     GIGLVSDVDDTIVHTGLTRGLEFLRVTLLTDVQDRTPLPGAAALYRALVRPADGPERP
                     VFYVSTSPWNLHEMLLQFVALRGFPIGPLLLTDWGPSHTGLFRIGAQAHKLGLVRRLL
                     DEHPHLRLVLIGDSGQEDPEIYASLAREVPDRVAAVYIRRTTGIDPVRAGQVDALAAE
                     ITAAGVPMLAVDDSAQIAVHAAGIGLLDAGAVDAVRAELNTS"
     gene            complement(6935..7948)
                     /locus_tag="VFE39_14455"
     CDS             complement(6935..7948)
                     /locus_tag="VFE39_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HET6259842.1"
                     /translation="MTATGAWPRCSFIADAADDPREGTAPPADRWFLVEHPGPWGRVA
                     LAQSGVHPAAVAAISAWAAAEGGRAVLVRKPGRAPRNETPARPGPGLLPGMRDVIDGA
                     GTPIGGRRRWFRVDSRPGHESIRTGTFVDESELVPAVHAPGERYAGQLSLVCAHGRHD
                     TCCAVRGRPLAAALTAAEPGSVWESSHIGGCRFAPALVLLPHGYVFGGLPAAEGPAVV
                     RAYAEGRVEPRWLRGRSSLPPAVQAAQHHCRAATGASGVDALRLVDVKSEQQADGSRV
                     WHVELSDPACSVRLRERRVETGRPLTCAATAPGWMRLFDLIELTAPGAVDHRPRSSGH
                     TAG"
     gene            complement(7945..9138)
                     /locus_tag="VFE39_14460"
     CDS             complement(7945..9138)
                     /locus_tag="VFE39_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6259843.1"
                     /translation="MTGLDAATFGAQHWGREPRLVRGADPDAFRDLLDLDGVDELLSR
                     RGLRTPFLRLARNGALVDSSSFTGPGGVGAEIGDQVRDDKVAPLFAEGTTVVLQALHR
                     TWPTVIDFATLLAEELGHPVQANAYVTPPSSRGFSAHYDVHDVFVLQLAGSKRWTVHA
                     PVHPDPLRDQPWNAHANAVAARARDDAPVIETVLAPGDVMYLPRGWLHAATALGEVSA
                     HLTVGVHVVTRFALVEALTALVAHDEELRASLPLGIDVADPGQLAPHLDAVRSALAGA
                     LERVTAEDVAHRVRGRVWTGGRPEPVRPVALAAFIEDLAAGDGVRRRTGLRHRLVRRG
                     EHVVLDLPDRQITLPAATEGAVRALASGDTHAVGSLPGMDEADQLVLVRRLLREGVLV
                     PATHS"
     gene            complement(9218..9625)
                     /locus_tag="VFE39_14465"
     CDS             complement(9218..9625)
                     /locus_tag="VFE39_14465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatC protein"
                     /protein_id="HET6259844.1"
                     /translation="MALDDEDMTTTAGGGNEGPADAGAGGGTPGVSDGGADGGADSGA
                     AGPADAGAGGGTPGVSDGGADGGADSGAEGPADAGAGGGTAGVHDGAADGGAEGPADA
                     GAGGGTPGVSDGGADGGAGGDGGADGGADSGAR"
     gene            9821..10834
                     /locus_tag="VFE39_14470"
     CDS             9821..10834
                     /locus_tag="VFE39_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HET6259845.1"
                     /translation="MLEITGLTWALTIGLVVALLALDLVLAAMRPHRVGFREATAWSL
                     FYIGVAIAFGVWFAMAYGSAAGTQYFAGYVVEKSLSVDNLFVFVIIMTTFAVPEEHQH
                     KVLTYGIVLALILRAIFIALGATLLSLFSFMFLLFGLLLIYTAVQLFRHRNEDPDVED
                     NAVVRTARRVLPITPEYVGGRIVTRVDGRLMATPLLIVLLAIGSIDLLFALDSIPAVF
                     GVTQEAYVVFAANAFALLGLRALFFLVKGLLDRLVYLSTGLSIILAFIGVKLILHWAH
                     EDLNASIPEISTPVSLGVIVVVLVVVIVASLIKTRGDPAAKAHAGSLRARKAAAEQER
                     DGA"
     gene            complement(10856..11188)
                     /locus_tag="VFE39_14475"
     CDS             complement(10856..11188)
                     /locus_tag="VFE39_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HET6259846.1"
                     /translation="MAAEQDPERVGRYVRMVAQPGQGAALADTLLRVATGLRDAPGCE
                     LYVINLSADEPDTVWVTEVWADEAASDRALSADLGEVGIGEVLELLAGPPELVDLRPL
                     GGAGLPSG"
     gene            12680..12772
                     /locus_tag="VFE39_14480"
     CDS             12680..12772
                     /locus_tag="VFE39_14480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="putative leader peptide"
                     /protein_id="HET6259847.1"
                     /translation="MDTAPQRPDVPLVRRYHVDLCRTSTAVCPR"
     gene            12866..14143
                     /locus_tag="VFE39_14485"
     CDS             12866..14143
                     /locus_tag="VFE39_14485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HET6259848.1"
                     /translation="MSVETPARPGSSRSLLSPAPTCAPPPAPGPAPSVRWAPLVAFVV
                     VGIALTAVVATGYGLRQAVLLLLGLGLGFALLHSRFGFTSAWRQLVAVGNGAGLRAHA
                     LLLGTTATLFALVIGTGAGLFGSTPAPSAGPLGIALVFGAFLFGMGMQLGGACASGTL
                     FAVGSGQGTIVLTLFGFVAGSVLYTWAYPLVADLPALPAVVLAEHIGWFGSWAVTIAA
                     LVAVVLVSRVVQARRNPPPVDVVPTARGAARIVRGAWPMLAGAVVLAVLGAGVLVVSG
                     GAWGITSAFGLWGAKLLQTLGLHPETWAFWQQPAQAKMLAGPVLADKTSLTDFGIMIG
                     AGIAAASAGAWFLHRSIPWRTALAAVIGGVIMGVGARLANGCNIGAYLAGIASGSLSG
                     WVWGLAALAGTWVGLRGRALFGLSTPAPSDSVC"
     gene            complement(14192..14395)
                     /locus_tag="VFE39_14490"
     CDS             complement(14192..14395)
                     /locus_tag="VFE39_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259849.1"
                     /translation="MELVDGLLASGDLARHHLAHATRADLLRRLGRIPDAVDAYRRAR
                     ELATAEPERRFLDRRLHELGAQH"
     gene            complement(14455..15570)
                     /locus_tag="VFE39_14495"
     CDS             complement(14455..15570)
                     /locus_tag="VFE39_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259850.1"
                     /translation="MPSQKVLDRPIRWEAEDSQERNARVGLLADPGLPTEIAEQVAEE
                     LPNMLADQPRDGMRYTFTVVSETLRRHGDSRGERLIDVAAKRRAREGWDIAICLTDLP
                     VHKDGRPVVAELSREAGAAVLVVPALKVLNPAEQARDIIAGLILEWAAGVGADEGLER
                     LEDRVTSLHRVEPADDDIDLQLTKSGGQLRMLVGMVRVNRPWRLVLGLRGALAAALAT
                     AAFGLVSAPVWQVSGAMSTVRLWIATAVSLAAIIGWLILNHGLWQHPSGIDPRERHRI
                     RLYNAATLLTLAIGVLVSYMVLLVGNFLTGWFVVVPEVFSQAIGRPVSFADYVRLAWL
                     VSSLATIGGALGSGLESTDTVRSATWGTRYRKNRKNH"
     gene            complement(15715..16173)
                     /locus_tag="VFE39_14500"
     CDS             complement(15715..16173)
                     /locus_tag="VFE39_14500"
                     /inference="COORDINATES: protein motif:HMM:NF026010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259851.1"
                     /translation="MTRRVEHWGTSRTAAQQALQEELRGRRGERTGMLKSNSRFRQAA
                     DIWMGTIKERRADSTADAYASCVKNQVLPQLGELRLVEWDVAHLDAFFSWLERARRVA
                     AQDDGTTVEKVRHSTRRMIRHFAVDPRGGLSGPQSVISRHNADQPQTDER"
     gene            complement(16354..16429)
                     /locus_tag="VFE39_14505"
     tRNA            complement(16354..16429)
                     /locus_tag="VFE39_14505"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16394..16396),aa:Lys,seq:ctt)
     gene            complement(16511..16586)
                     /locus_tag="VFE39_14510"
     tRNA            complement(16511..16586)
                     /locus_tag="VFE39_14510"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16551..16553),aa:His,seq:gtg)
     gene            complement(16622..17257)
                     /gene="orn"
                     /locus_tag="VFE39_14515"
     CDS             complement(16622..17257)
                     /gene="orn"
                     /locus_tag="VFE39_14515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HET6259852.1"
                     /translation="MNDRLVWIDCEMTGLDLQRDALIEIAVLVTDGDLNVLGRGVDVV
                     IHADESALAGMPDVVREMHAHSGLTEAVRASTVTVAEAEALALSYVREHVPEPRTAPL
                     AGNSIATDRGFLSRDMPELDNHLHYRMVDVSSIKELCRRWFPRVFYAKPEKGLAHRAL
                     ADIEESIRELQYYRRTLFVPEPGPTTEEAQAAAAQVAGPVATRFDTAEPPR"
     gene            17360..18925
                     /locus_tag="VFE39_14520"
     CDS             17360..18925
                     /locus_tag="VFE39_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HET6259853.1"
                     /translation="MENTIGADQSSAAREIAAGYATEGAALELGAVVVDGVVDPEARV
                     RIPFATLNRHGLVAGATGTGKTKTLQGIAEQLSAAGVPVLLADIKGDVSGMARPGEPG
                     PKIESRARDTGDDWTPTAYPVEFLSLGGTSSAVPIRATLTQFGPILLSKVLDLNDTQE
                     STLGLIFHWADQRGLPLLDTKDLRAVVQHLTSGEGKADLKGIGGVSPATAGVILRALV
                     NLEAQGGEDFFGEPEFEPADLIRQVDGKGVVTLLELSDQAANPRLYSTFLMWLLAELF
                     EELPEAGDLDKPKLVFFFDEAHLLFTDASKAFLERIEQTVKLIRSKGVGVFFCTQLPT
                     DVPNAVLSQLGARIQHALRAFTPNDQAALAKTVRTYPNTKTYDMEEALTTLGTGEAIV
                     TVLSERGAPTPVAWARMRAPRSLMAAIGADAVTAAAKASPLYGKYGQTVDRESAYESL
                     TAKIAATPGPEAAPADVPPPLREPERRRERDEPSMVAEVLASPVVKSFLRSAASALGR
                     EITRGMFGTRRRR"
     gene            complement(19001..20617)
                     /locus_tag="VFE39_14525"
     CDS             complement(19001..20617)
                     /locus_tag="VFE39_14525"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET6259854.1"
                     /translation="MTSDTDAPTTIETPWRRLDKRMLVVSPIAGLVRLLPVAVILLVT
                     GQRGDPMRLWIPLGIGVLVVIGGVVRWRTTQYRITGDRVELHTGWLRRQRRSVPRDRI
                     RTVDLTAKLLHRIFGLSVVHIGAASGSPTENAGLTLDAVSKAEADRLRRELLDRSLIA
                     SGPTATGPTANHPAPPPATELARLRWSWLRFAPLTFSAIAGVGAIGAAVFNLFGELGV
                     SPRDISAVDDAADRLTTTPIWVAVAVLAALLLVVAVIGAMVLFAERWFGYQLTREAAA
                     DGPGWDPAGATASTGIGTGTLRVRRGLLTRRSLSVSEQRLRGVEVVEPLLLRAGHGAY
                     SKALSTGLTRSAQGGALQPPCPRAEAHRVAAAALREHRHEVTLAPLRRHPRAALRRRL
                     TRALAPTAVLVAGVFAAGAAVPVLAGLGLPSLLLLPLAGLVALDRYRNLGHELTTRYL
                     VSRHGSVQRRTVALQRAGVIGWTFRQSLFQRRAGLVAVEAITAAGAGGYEVLDIAAAD
                     GVALADAAVPHLLTPFLAAATPEEPQLDHR"
     gene            complement(20614..21123)
                     /locus_tag="VFE39_14530"
     CDS             complement(20614..21123)
                     /locus_tag="VFE39_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET6259855.1"
                     /translation="MAIATTPALPLRPPAHQVSPRAVRWWQVRALLTLVVLVAPQVVV
                     LLVLGSRAPGWLAYTAAATAAVTLVYAIVVPPLLYRIHRWEVTPEAVYTLSGWLVREW
                     RIAPISRVQTVDTEHGPLQQLLKLASVTVTTASARGPVKISGLEAQAAAELARTLTET
                     TQATPGDAT"
     gene            complement(21183..22334)
                     /locus_tag="VFE39_14535"
     CDS             complement(21183..22334)
                     /locus_tag="VFE39_14535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6259856.1"
                     /translation="MSAAAASVEALFAPEPRVAEHPAVRAARQIAADVLAEHAEAADD
                     PARGVDPAHLRLLAEHGLLSVKIPAAEGGHGADDRVDAETVELVSGACGATWFLTTQH
                     RFPQGLSRGPLPGLDAGAIELGPAAARHRAGLAAATTSAGIAIAHIRRPGPPAVRAEA
                     RPGGWTFSGTADWCTGWGLIEVVMIAASTTDDRFVLALLPATERPGLRPGPRLPLAVM
                     GGTRTVALEMDGMQIDAEDVLATVDVRAWRAHDAARTANTTPASLGLLRRMLVALAEL
                     GTAKARPEAVDLAMTLAERAADRRTEAYALLTTVPLVERVPERTALRAEVTELAVRTA
                     NALIAARSGSALLLASPEQRWAREAAFHLIQAQTAGVRQAQLAAFAHPR"
     gene            complement(22349..23236)
                     /locus_tag="VFE39_14540"
     CDS             complement(22349..23236)
                     /locus_tag="VFE39_14540"
                     /inference="COORDINATES: protein motif:HMM:NF018677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrase ferredoxin"
                     /protein_id="HET6259857.1"
                     /translation="MDAETAAGARCALLAHALDEPLVGTAPVAPRWVCVEHRGAWPRD
                     VEDHPDPAIATFAARARAAGWRLLLIRRPGRRCDETGGPTRVYITDTAPPIARTARLM
                     VAGPSELADLALPAVGDVLPGAVVAEPLLLVCTHGRRDRCCALDGRALALAVAAGSEL
                     DVWECSHLGGHRFAPTALVLPTGYTYGRLDPAGAVAAHKAVSSGEVETGLCRGRSTWS
                     PAGQVAELAVRAATGLRDAHALQVSDVSGGAIVTARDGGRWAVDVAAVDGPGLRPASC
                     GADAVPYAALRAIGLRRLA"
     gene            complement(23284..24903)
                     /locus_tag="VFE39_14545"
     CDS             complement(23284..24903)
                     /locus_tag="VFE39_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6259858.1"
                     /translation="MIDVLTRVLGTVRSGAEQAVGSARAVVALTRSGVLRVERPDRLL
                     GIGEGLLRYGFSIAAGYVVGAARHPDRTAIVDDDGAVTYAELHRRTDAMARGLAAAGV
                     GPGVRVGLLCRNHRGAVETQVAVGKLGADVVLLNTGLSAGQLSEIAVELQLHTVVADP
                     EFDAALKGLPDGCTVVHDLTELDGPGPLPWRPPAGRTIVLTSGTTGTPKGARRPPPHS
                     LAPAAAILSTIPLRAAEPVLIAAPLLHTWGSAALQLAVLHGAPVVLRRRFDPAEFLRV
                     VEEQRCDAVFAVPVMLQRLLELPAAQLDAAWSRHRPRVVAVSGSALPPQVAAAFLDHF
                     GDVLYNLYGSTEVSWVSIASPADLRAAPGTAGRAPPGTRLVVLDDNGRPVPPGTEGRI
                     FVRNDMPFEGYTREGADVEREGRLLGTGDIGRLDEDGRLLITGRADDMIVSGGENVHP
                     GPVEDLLAARPEVREAAVTGVPDEQYGQRLAAYLVLEPGTSVDPDDARGWVRDELGRF
                     SVPRDVEFVEELPRNATGKVVHRELRSPDRR"
     gene            complement(25157..27040)
                     /locus_tag="VFE39_14550"
     CDS             complement(25157..27040)
                     /locus_tag="VFE39_14550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020666335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HET6259859.1"
                     /translation="MTATLAPTGPAPVTVAPPPRRRYERLALAALLAGTAALYLWALG
                     ASGYANSYYAAAVQSMTRSWESFLFGSFDTGNIITVDKPPASLWVMALSGRIFGFSSW
                     SMLVPEALMGVATVALMYAAVRRVSGPRAGLLAGAAMALTPVAVLMFRFNNPDALLVL
                     LMVAAAYATVRATEAAATRWLLLAGALLGLAFLAKMGQALIVVPALALAYLVAAPTGF
                     WRRIGQLLAGGIALVVAAGWWVATVELWPAADRPYIGGSQTNNVLELAFGYNGLGRIF
                     GGSGDPRMGGHGGGGVGGFGGPAGLTRMFGADVGAQVSWLLPAALALLVIGLGLTWRA
                     PRIDRTRASLLLWGTWTIVTALVFSLAEGIFHSYYTIALAPGIAALLGVGGHELWRHR
                     ARWPGRVALALVVAGTAAWAVVLLDRSPEFLPWLRWVVIGVGALAVLALLVRPAGRRW
                     VAVTALVVALTGFAAPAAFAVETAATPHTGSIPAAGPPTDATKVFLDRLRGNPHGLPL
                     GVAAMLGGPQQNIDPELVALLQQAGTKWSAATVGASGSAQLALSSGTTVMAIGGFTGF
                     DPAPTLEQFQSYVRAGEVHYFVADRFEGGRAGSLISTWVEDNFTHVTVGGQTVYDLTQ
                     ARI"
     gene            complement(27058..27228)
                     /locus_tag="VFE39_14555"
                     /pseudo
     CDS             complement(27058..27228)
                     /locus_tag="VFE39_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            complement(27326..28789)
                     /locus_tag="VFE39_14560"
     CDS             complement(27326..28789)
                     /locus_tag="VFE39_14560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6259860.1"
                     /translation="MLLLLLAVASTVVALVTALTLRGFLISRLDGDLRASSRVLTRLG
                     PLDPLLPPDAGGRLGPRPPDSLTAVLRDGSVVSAWVFTRDGGVQPVPPSEYPDLTSIT
                     VDRPPLSADLGPMGAYRLVAVSGGNGEVFVTGFTERPVRDTVARLVTTELIVATVTLL
                     GAGLAGVALVRRELRPLERVAATAARVSTLPLDRGEVSLVERVPAADPRTEVGQVGAA
                     LNHMLDHVGAALEARQDSEMRLRQFVADASHELRTPLAAIRGYAELSRRGELSDEAAY
                     ALARISAQSERMTTLVEDLLLLARLDAGRPLERAEVDLTRLVVDAVSDAHAAGPDHRW
                     QLALPEEPVTVPGDPLRIAQVLANLLANARTHTPTGTAVTVALAVEPTAARLDVVDNG
                     PGVPPELLPHVFKRFARGSTSRSRANGSTGLGLAIVDAVVTAHGGAVRVASAPGRTAF
                     TVHLPSDPPVRMPTRVLHLDPTGSPGTEPTPPRPQLR"
     gene            complement(28822..29562)
                     /locus_tag="VFE39_14565"
     CDS             complement(28822..29562)
                     /locus_tag="VFE39_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6259861.1"
                     /translation="MSTMRDQATALRRADGSAIRILVVDDEASLSDLLSMALRYEGWD
                     VRSAADGGSAVRAAREFRPDAVVLDVMLPDFDGLEVLRRMRAETPDVPVLFLTARDAV
                     EDRIAGLTAGGDDYVTKPFSLEELVARLRGLLRRSGMSAARQGSELVVGDLVLDEDSH
                     EVRRGDAPLELTATEFELLRYLMRNPKRVLSKGQILDRVWNYDFGGQSNVVELYVSYL
                     RKKIDAGRAPMIHTVRGAGYVLKPAAGG"
     gene            29683..30411
                     /locus_tag="VFE39_14570"
     CDS             29683..30411
                     /locus_tag="VFE39_14570"
                     /inference="COORDINATES: protein motif:HMM:NF020922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 family protein"
                     /protein_id="HET6259862.1"
                     /translation="MDTFEGWPADATTFLAEIAADNTREFWAEHRHRHATSVHSPMRA
                     LAAELEPEFGPVRVLRPFVNLRFRPDASPYRTDAGGVATTAGGCPLAVVVSAAGLSVS
                     AGHRSFDAGQLRRYRAALDTPAGAELAQLLAELDGYAVEPGRPLTGIPRGFRADHPRI
                     ALLRRRGVQLVRSRPAGEWLATREPLDWVRVAWRAAGPVVAWLDLHVGAAERVPLPRR
                     VPGPGVADSSTDTMKSVIVESADR"
     gene            complement(30420..31163)
                     /locus_tag="VFE39_14575"
     CDS             complement(30420..31163)
                     /locus_tag="VFE39_14575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-interacting protein"
                     /protein_id="HET6259863.1"
                     /translation="MTTTAPRLLQVRRSERISPRMIRVTLGGDELAGFGGDGPDRRIK
                     MFFPVPGQDRPAVPRATSGGPLWPAGEPRPAIRTYTVRRFDAAAGELDVDFVLHDGHG
                     PAAAWARGACPGSWVGVSEPGGRYEPDPAADFHIVIGDETALPAVATVLDALPAGVPA
                     LAFLEVADADEEQQLPGAATIRWVHRGDREPGVPLTAAVRSTELPAGAGQAWLSGESA
                     CVRDLRKHLLDDRGLDRRKVYATGYWRAR"
     gene            complement(31160..31771)
                     /locus_tag="VFE39_14580"
     CDS             complement(31160..31771)
                     /locus_tag="VFE39_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6259864.1"
                     /translation="MDTRTPRRRVSRAERERQILDAAVAVFGERGYQAASMDVVAERV
                     GVTKPVLYAHFGSKDGLLLACIARARAELLEVTTTAAASADGPEEMLRRGTLAFFEYL
                     ERREPAWTLLYSESTVAGEALEGIRAQQTDFIAALLAVQAPDADPQRLAGWAQVIVGA
                     CERLALWRGRERTVTSEQATEYLMDLVWTGLAAAGGAAAQSSR"
     gene            31858..>32646
                     /locus_tag="VFE39_14585"
     CDS             31858..>32646
                     /locus_tag="VFE39_14585"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6259865.1"
                     /translation="MSEPTADRSADVADAVVDIAIVGSGFAGLGAAVRLAQAGRNDFV
                     ILEKGESLGGTWRDNTYPGCACDVQSHLYSFSFAPNPNWTRLFAQQPEIRAYLEDIAD
                     RYGLRARIRFRSTVVAAEWGGRHWTVRMADGKSITARVLVWGTGALHEPSIPDIAGLD
                     EFAGTVFHSARWNHDHDLRGMRVAVLGTGASAIQFVPAIQPGVARLTVFQRTAPWVLP
                     KPDRAVSPLWRRVYQAAPVVQKLQRALIYARNESLLTGFRKPRRM"
BASE COUNT         4586 a        11794 c        11762 g         4504 t
ORIGIN      
        1 atagatggga taccgctcgg acaggcggtc ctcgcacgct agaaacgccc gtgacctgcg
       61 gtcaacggtc ctcgttcggc attgtcgaac caccaatcgg acgaacatca catgctgccc
      121 cacggggggc gggggtctcg cactgagtga aggcgtgtag cgctgcgtgt ctcacccgtt
      181 cggtgggtcg tacagtcggc tcgtgccggg gttcatccag tccatcgagc gagcggcagc
      241 ggtgctgcgg ctgctcggga ccacggaccg gccgctcggg ctgaaggaga tcgcagcggc
      301 cctcgacctg ccggccccca ccgcccacgg gatcgtccgg acgctgcgcg aggtgggttt
      361 cgtcgagcag gacatggaga cgtcccgcta ccggctcggt ccggggctgc gtgagctccg
      421 cagcggtggt tgggaccacc acgacctgcg cgcccgtgcc atgaactggg ccgactcgct
      481 cgccggcagc acggggctgg ccgtgcatct gggtgtcgcc gtgacgcgct ccgtccggct
      541 cgtccaccac gtgttccggc cggacgggtc cccacagcgg atccgaacgg gcgaggaaca
      601 gcccctgcac gccaccgcgc tcggcaagtg cctgctggcg ttcgcgccgg tggccactcc
      661 gcacgcccgg gagctggacc tgcagcgttg gaccggccgg acgtgcacca cggtccccgc
      721 gctggaggcc cacctcgcgg tcgcccgccg ccgcggctgg gcgagcgaca ccggcgagta
      781 cgaggcgggg gtgggtggtg cggccgcgcc gttgcgggcc agcggtggga tcgtcgtcgc
      841 ggccatcggg gtggtcggtc cggtcgagga gctgttcggc ctgggcgggg agttgcggcc
      901 gtggctgctc gagcagatgg tgggcgctgc acgggagatc tcggcggcgc tggtggaggg
      961 ccaatgacac ggctgacgcg cagcgccaca gttcgccgcc gccggcacgg accggcacac
     1021 cgatgacgga gcgcgtcgtc gccgcgatcg accagggcac cacgtcgacc cgctgtctgt
     1081 tgttcaaccg gtcggggcgg atgctcgccg tggcacagcg ggagcaccgc cagcactacc
     1141 cgcggcccgg gctcgtcgag cacgacgccg cggagatctg gcgcaacgtc acgcgggtcg
     1201 tcccggcggc gctgcgcagt gcgggcctca ccccggacaa catcgtcggg ctcgggatcg
     1261 cgaaccagcg cgagaccacc gtcatctgga accagcacac cggtgtcccg ctggcgcgcg
     1321 cgatcacctg gcaggacacc cgcaccgacg gcatcgtcgc gcgcctggcc gaggacgggc
     1381 acgccccgct gatcaaggag atctgcggcc tgccacccgc cacctacttc gccggcccac
     1441 ggctgcgctg gctgctcgac cacgtgccgg gcgcccgtga gggtgcggag gcgggcgagg
     1501 tcctgttcgg cacgatggag agctggctga tctggtggct caccggtggc ccggacggcg
     1561 gcaggcacgt cagcgacgtc accaatgcca gccgcacgat gctcatggac gtccggacgt
     1621 tggaatggtc ggacgaactc ctggacgtgc tcgcggtgcc ccgccggatg ctgcctgaga
     1681 tccgtccgaa cgccgaggtg tacggcacct gcaccgccgt gcttcccggc gtcccggtcg
     1741 cgggcgcgct cggcgaccag caggcggccc tcgtcgggca ggcgtgcttc tcgcccggcg
     1801 agatcaagtg cacctacggc acgggcgcgt tcctgctcat gaacacgggg ccgcggttgg
     1861 ccgagtccac ccacggcctg atcccgaccg tcgggtacct gctcggcgac aagcccgtct
     1921 acgcgctgga gggcgccatc gccatgacgg ggtcgctcgt gcagtggttc cgcgactcgc
     1981 tggggatgat cggcacggcc gcgcagatcg agacgctcgc cagttccgtc cccgacaacg
     2041 gcggctgcta catcgtcccg gcgttctccg ggctgtactc accgcgatgg caccccgacg
     2101 cgcgcggcgt gatggtcggg ctgacgtcgt tcatcaaccg cggccacctc gcccgcgccg
     2161 tgctcgaggc caccgggtgg cagacccgcg aggtgatcga cgcgatgaat gccgactccg
     2221 gggtgccggt cacccggctc aaggtcgacg gtggcatgac ggcgaaccac ctgctcatgc
     2281 agttcgtggc cgacgtcctc gacgtgccgg tggagcggcc gctggtgtcc gaggccgtct
     2341 cgctgggggc ggcgtacgcg gcggggcttg ccgtcgggca ctggtccgac ctggaggtgc
     2401 tgcgctcgaa ctggcaccgc gcggccgcct gggagcccgc gatggacgcg gtgctgcgcc
     2461 gccgcgagca cgagaactgg ggccgagccg tgagccgcag ttacgactgg gtgcagacgc
     2521 cggcgggcga ctgagcagcc aggccgtcag gccgtccggg tgcggcgacg gctcaccacc
     2581 gtgtgcagga ggaaggcgat cccggcgccg atgaaccagc tgtatgccgc ggcttcggac
     2641 gatccgaaga ggaccacgac catcgcgagt accgcgccga ccgccgtcgc cccgatcgca
     2701 gtggggttgt agccgcggtg gaagtggtag ctgccgtcgg accggagcgt gtagaggtcg
     2761 tcgacgacga ttcggcgctt cttgatgagg tagtagtcgg cgatcagcac gccgtagagc
     2821 gggccgatga acgcgccgag cgtatcgagc gtgtagtgga tgacctcagg actggagtag
     2881 aggttccacg gcgtgatcag cacggacccg acggcggcaa tcattccgcc ggcccgccag
     2941 ctgatcttct gcggattgac gttggagaag tcgaaagcgg gcgagacgaa gttggcgacg
     3001 atgttgatgc cgatcgtcgc gatcatgaag gtgagggcgc cgagcaccac cgcgtaggcg
     3061 ttgtcgagcc gggcgacggt ctcgacgggg tcggtgatga gctcgccgaa caccggctgc
     3121 gtcgcggcgg cggtgaccac gaccagcagc gcgaacacca ggaagttcag cggcaggccg
     3181 agcaggttgc cccggcgcac ggccgcgaag ctgcggccgt agcgggcgaa gtcaccgtag
     3241 ttcagcatcg gcccggagaa gtaggacacg acgagcgcga tcgccgtgag catcaccggc
     3301 accgcagccc acccggtgat gctcacctcc gcgaggttga ggtcgatgtt gctccagccc
     3361 gccgccatca cgaggtagcc ggccagcagg aacatcacga cgtacacggc aggcccgcag
     3421 aagtcgatga acttccggat cgcctccatg ccgttccaga acacggcggc ctgcagcacc
     3481 cacatcgcca cgaacgcgat ccagcccagc agcgacagcc cggcgaaccc gtactgctgg
     3541 gcgtccgcgt agggcaccag acgcggccac agcgtcagcg cgacgatcac cagcgaatgc
     3601 gaggcgaggt aggtctggat gccgtaccag gccaccgcga tcagtccccg gatgatcgcc
     3661 ggcacgtttg cgccgagcac tccgaacgac gcccggcaga tcacgggata cggcgtgccg
     3721 gtcgcctggc tgggtcgcgc gacgaggttg cacaggaggt agacgatcaa gatgccgatg
     3781 agcagcgcaa cgagcacctg ccacgccgcg aggccgaggg cgaacaggct gcccgccgtc
     3841 acgtacccgc cgacgctgtg cacgtcggac atccagaagg cgaagaagtt gtacgaggtc
     3901 caccgctgct cccgcagggg cgcgagatcc tcgttggtga gccgtgggtc gtattcgggg
     3961 gtgtcgtggg cggcagcctc gcgcgctgct ccggcatgtt cgagcgaatt tgtcatgctg
     4021 accccccgga ctcggcgatc gtgcaccgga accgtagatc gctacccggg cgcggagcgg
     4081 gccgttacga gatcttcccg ctggtgaaac atctgccgcg tgtcgtcagg tgatcgtgaa
     4141 acctccgagc aacccgacat aggacttcgg tcgtggtgcc gcgaactcgc cgcgcttgcc
     4201 cgtgcgcagc ccgagcgcca ccagcgactc ggcggtacgc gtggcggccg cgacaccgtc
     4261 caccaccggc acgccgattc gggcgctgat gtccgcgcag aggtccgcca tcccggcgca
     4321 gcccagcacg atcacgtccg agccgtcctg cgccaccgcc cggcggcact cctcggtgat
     4381 cacctcgcgc gcatcggaac ccggggcgtc cagctccagc acggggatct cgcaggcccg
     4441 cacgttgcgg cagaagcggc tgacgccgta ccgctcggcg aggtcccagg cccgcccgca
     4501 cgtccggccg agcgtcgtga ccacgctgaa gccgcgcccg aggtacgccg ccgtgcgcat
     4561 ggccgcctcg gcgatcccga cgacaggacc ggcggcgagc tcgcgggcgg cgtcgaggcc
     4621 ggggtcaccg aaacacgcca cgacgtaccc gtcgacgccg tcggcctcgc cgcgggcgat
     4681 ctgctcgagc agccccggca cggcgagtgc ctcgtcgtag tggctctcga tggacaccgg
     4741 ccccatgtcc ggggacacgg cgagcaccat ggtgcccggc gacgcggcag cctgcgcgca
     4801 cgcgccgatc ttgtccgtaa tcgtcgcggt cgtgttgggg ttgacgacca tgatgcgcat
     4861 ggcgctcacg gacgcggccc gttcacgaac gctgcgccgg cggcaccgtg gaccacacca
     4921 cgtcgccgcg cagtgcctcg gccagcttct ggacatcggt caccaccagc cgggcgctgt
     4981 cgagctcgcc gggctccgcg tacccccacg ccaccccgat cgtcgggatg ccgtgctcgg
     5041 ccgctccgtc gacgtcgtgg tggcggtcgc cgaccatcac gaccggctcc cgggccgggt
     5101 cgacgccgag ccgctcgatc accgacccga cgacggcggc ctttcccacc cggccggcca
     5161 cgtgatcggc gcctccgacg agctcgatgt atccggccag cccgactccg gcgacgatct
     5221 tgcgcgcgag ctgctccggc ttcgatgtcg cgatcgccag ccgcaggtcc gcctcccgca
     5281 gccgctccag cagcgcgggc acgccggggt agaccggggt gactgtggcg gccaccgcgt
     5341 catacgaggc gcggtacgcg gtgatcacgg cggaggtctg ctcgtcggtg agaccgagct
     5401 gcggcagtgc ggtctcgaac ggtggtccga cgagtcggcg caaccgttcc gcgtccggca
     5461 ccgggaagcc gacctccgtc agagccgccg cgaggtgctc ggtgatcgtg atggcggagt
     5521 cgacgagcgt gccgtcgagg tcgagcagga cagtgcgcac cggtccgagc ctacgacccg
     5581 gcgccgggac cgccgggagc caccgctcgg acacccgatg tcgtcgccgc gtcggcgggg
     5641 tcgtgcaccg gtgacgctca gcgaccccgg ggtggcagtt gagcgccacg tgcggtagcc
     5701 cttctcccgt gactggaccg gactggggca agctgatcat cgccgtggga ctgcgggtgg
     5761 aggacgccgc cgagtcggtg atcgcggcgt tggcccgcct gcgtggcagg cgacacccgg
     5821 tgatcgtccc gttcatcggt cacggcacca cccggcgggc ccgcgttgga gctcggctcg
     5881 tgctcggacg cgatgccgcc gctccgtccg tcctggcatc ggctgcgggt ggggatgcac
     5941 cctcgcagcg gtcgcgcagg gccgtgctgc gggcgagcat cgcacggttc ctcaccatcg
     6001 aggtgccgca cgccgaggtg acgatccacc tccccggcgg cgacgtcgtc gtccacaccg
     6061 accgcgacgg ctacgtcgac cacgaggtcg aactgcccga cgttgctccc ggctggctgg
     6121 aggtcgggct gaccggaccc gcgggatcct ccgcgaccgc gcgcgtgctg gtggccgatc
     6181 ccgaggcggg catcgggctg gtgagcgacg tggacgacac gatcgtgcac accggcctca
     6241 ctcgcggtct ggagttcctg cgcgtcacgc tgctcaccga tgtccaggac cgcacgccgc
     6301 tgcctggcgc ggccgccctg taccgggcgc tcgtcaggcc ggcggacggc ccggagcggc
     6361 ccgtcttcta cgtctccacg agcccgtgga acctgcacga gatgctcctg cagttcgttg
     6421 cgctgcgcgg gttcccgatc ggcccgctgc tgctcacgga ctgggggccc tcacacaccg
     6481 gcctgttccg catcggagca caggcccaca agctcgggct ggtgcggcgc ctgctcgacg
     6541 agcacccgca cctccggctc gtcctcatcg gcgacagcgg gcaggaggat ccggagatct
     6601 acgcgtcgct cgcacgcgag gtccccgacc gggtggccgc cgtgtacatc cgccgcacca
     6661 ccgggatcga cccggtccgc gccggtcagg tcgacgccct cgccgctgag atcaccgcgg
     6721 ccggagtgcc gatgctggcg gtggacgaca gtgcgcagat cgccgtacac gccgcgggca
     6781 tcgggctgct cgacgcgggc gcggtggacg ccgtccgcgc ggagctcaac acgtcctgaa
     6841 ccacgaccgg cccagcgagc cgagctgttc agcgcgccct gggtacgcct ggtacatacg
     6901 gccggccggc gacccgccga gacggcgcag tcgatcaacc ggcggtgtgg ccggagctgc
     6961 gggggcggtg atccactgcg ccgggcgccg tgagttcgat cagatcgaac agccgcatcc
     7021 agccaggggc cgtggcggcg caggtcagcg ggcggccggt ctcgacccga cgctcgcgga
     7081 gccgcaccga gcacgctggg tcgctcagct ccacgtgcca gacgcgactg ccgtccgcct
     7141 gctgctcgct cttcacatcg acgagccgca acgcatcgac gcccgacgcc ccggtggccg
     7201 cacggcagtg gtgctgcgcg gcctggacgg cgggtggcag tgacgagcgg ccacgcagcc
     7261 accgcggctc gactcgcccc tcggcgtagg cccgcacgac cgcgggcccc tcggcggccg
     7321 gcaacccgcc gaacacgtag ccatggggga gcagtaccag cgcgggggcg aaacggcagc
     7381 cgccgatgtg gctggactcc cagacgctgc cgggttccgc tgcggtcagc gccgcggcga
     7441 gtgggcgtcc gcgcacggca cagcaggtgt cgtggcggcc gtgtgcgcag accaggctga
     7501 gctgcccggc gtaccgctca cctggagcat gaacggccgg caccagctcc gactcgtcca
     7561 cgaaggtgcc cgtgcggatc gactcgtgcc ccggccgcga gtccacccgg aaccagcgcc
     7621 gtcgcccacc gatcggtgtc ccggcgccgt cgatcacgtc gcgcatgccg gggagcaacc
     7681 ctggtccggg tctcgccggg gtctcgttcc tcggggcccg ccccggtttg cgcacgagca
     7741 cggcccgtcc gccctccgcc gcagcccatg ccgagatcgc cgcgaccgcc gcagggtgga
     7801 cgcccgactg cgcgagcgcg acccggcccc acggcccggg gtgctcgacc aggaaccagc
     7861 ggtccgcggg cggcgccgtg ccctcgcggg gatcgtccgc cgcgtccgcg atgaacgagc
     7921 agcgcggcca cgccccggtc gccgtcacga gtgagtggcc ggcaccagca cgccctcccg
     7981 aagtaggcgg cgcacaagca cgagctggtc ggcctcgtcc atgcctggga ggctacctac
     8041 ggcgtgggtg tccccgctcg ccagtgcgcg caccgcaccc tcggtcgccg ccgggagcgt
     8101 gatctggcga tcgggtaggt cgagcacgac gtgctcgccg cgccggacga gccggtggcg
     8161 caaccccgtc ctgcgccgga cgccgtcgcc ggcggcgagg tcctcgatga acgcggccaa
     8221 agccaccggc cgcacgggtt cggggcgacc gcccgtccag acccggccgc ggacgcggtg
     8281 ggccacgtcc tcggccgtca cccgctccag ggcgccggcc agggccgagc ggacggcgtc
     8341 gaggtgcggg gcgagctgcc cgggatcggc gacgtcgatg cccaggggga gcgacgcccg
     8401 cagctcctcg tcgtgcgcga cgagcgcggt caaggcctcc accagcgcga accgcgtgac
     8461 gacgtgcacg ccgaccgtca gatgcgccga cacctcaccc agggccgtgg cggcgtgcag
     8521 ccaccctcgc ggcaggtaca tcacgtcgcc gggcgccagc accgtttcga tcacgggagc
     8581 gtcgtcgcgc gcgcgggcag ccaccgcgtt cgcgtgtgca ttccagggct ggtcgcgcaa
     8641 cgggtcgggg tgcacggggg cgtgcaccgt ccagcgtttg ctccccgcga gctgcaggac
     8701 gaacacgtcg tggacgtcgt agtgggccga gaatccgcgc gacgacggcg gcgtgacgta
     8761 cgcgttggcc tgcaccgggt gcccgagctc ctcggcgagc agcgtcgcga agtcgatcac
     8821 ggtgggccag gtgcggtgca acgcctgcag caccacggtg gtgccctcgg cgaagagcgg
     8881 ggccaccttg tcgtcgcgca cctggtcgcc gatctcggcg cccaccccgc cgggccccgt
     8941 gaacgagctg gagtcgacca gggccccgtt gcgcgccagc cgcaggaacg gcgtgcgcag
     9001 cccgcggcgc gagagcagtt cgtcgacgcc gtcgaggtcg agcaggtcgc ggaaggcgtc
     9061 ggggtcggcg ccgcgcacga gccgcggctc ccgtccccaa tgctgggcgc cgaaggtggc
     9121 ggcgtccaga cctgtcacgc gggcgactgc ggttggcgac gcgggtgccg aacgccccgt
     9181 tacgggccgc ccggcaccat gcgctggttc ggccacctca gcgggcaccg ctgtccgctc
     9241 caccatccgc tccaccatcg ccgcctgcgc ctccgtcagc gccgccgtcg gagacgccgg
     9301 gcgtgccgcc gcccgctccg gcgtcagccg gcccttcggc tccgccatct gccgcgccgt
     9361 cgtggacacc agcggtaccg ccgcctgcgc cggcgtcggc gggcccctcc gcgccggagt
     9421 cggcgcctcc gtcagcgccg ccgtcggaga cgccgggcgt gccgccgcct gctcccgcgt
     9481 cggccggacc tgcagccccg ctgtccgcgc cgccgtccgc accgccgtcg gagacgccgg
     9541 gggtgccccc gcccgcgccg gcgtcggcag gtccctcgtt cccgccgccg gccgtcgttg
     9601 tcatgtcctc gtcgtcgaga gccactgcgt cctcctctag tgatccgtca ccacagcgat
     9661 gacgagcggg gactaccccc ttgaccaccg atgtagaccg catgatcaac ttcgaaccgg
     9721 tgattcgctc cgccccgccc gaatgcggtg gaaccgcgct cgcgccgccc ccgttgggta
     9781 aatcgtcttt aatgccgatg gcgacggtgt ggaggtacag gtgctggaga tcactgggct
     9841 cacctgggcg ttgacaatcg gtctggtcgt cgcgctcctg gcgctcgacc tcgtactggc
     9901 ggcgatgcgc ccccaccggg tcgggttccg tgaggccacg gcgtggtcac tgttctacat
     9961 cggcgtcgcc atcgcgttcg gcgtctggtt cgcgatggcc tacggcagtg ccgccggcac
    10021 gcagtacttc gccggttacg tcgtcgagaa gagcctctcg gtcgacaacc tgttcgtctt
    10081 cgtgatcatc atgacgacgt tcgcggtgcc cgaggagcac cagcacaagg ttctgacgta
    10141 cggcatcgtt ctcgcgctga tcctccgtgc gatcttcatc gccctcggcg ccacgctgct
    10201 ctcgctgttc tcgttcatgt tcctgctgtt cgggctgctg ttgatctaca cggccgtgca
    10261 gctgttccgg caccgcaacg aggacccgga cgtcgaggac aacgccgtgg tccggaccgc
    10321 ccgccgggtc ctgccgatca cccccgagta cgtgggcggg cggatcgtca cgcgcgtcga
    10381 cggccgcctc atggcgaccc cgctcctcat cgtgctcctc gcgatcggca gcatcgacct
    10441 gctgttcgcg ctcgactcga tcccagcggt gttcggcgtg acccaggagg cctatgtcgt
    10501 cttcgcggcc aacgcattcg cgctgctcgg cctgcgggcg ctgttcttcc tggtgaaggg
    10561 cctgctcgac cggctggtgt acctttccac cggcctgtcg atcatcctgg cgttcatcgg
    10621 cgtcaagctg atcctgcatt gggcgcacga ggacctgaac gcaagcatcc cggagatctc
    10681 cacgcccgtg agcctcggtg tgatcgtggt ggtgctcgtc gtggtcatcg tggcgagcct
    10741 gatcaagacc aggggagacc cggcggcgaa ggcccacgca ggctcgttgc gggcccggaa
    10801 ggcggcagcg gagcaggaga gggatggagc gtgatgctgc gcgcgccgac cgcgttcagc
    10861 cggacggaag tccggcgccg cccagggggc gcaaatccac cagctccggt ggccccgcca
    10921 gcagctccag cacctcaccg atgccgacct ccccgaggtc ggccgacagc gcccggtcgg
    10981 acgccgcctc gtcggcccac acctcggtga cccagacggt gtcgggctcg tcggcggaga
    11041 ggttgatcac gtagagctcg cagcccggcg cgtcgcgcag cccggtggcc acccggagca
    11101 gcgtgtcggc cagtgccgcc ccttggcccg gctgcgcgac catccgcacg tagcgtccga
    11161 cccgctccgg gtcctgctcg gctgccatcg gacgaccgta gcggcgccgc cggcatgggc
    11221 ccggcccggg tgtggcttga gacaagtctg cggcgtcccc ggcagggggc ccgcagtttc
    11281 tggtggtgcg cccgcgaggg aatcaaagcc caacctcgca caagggccgt gcgcgcggaa
    11341 aagggattct cccgccggga gtggccccgc ccaaggcggg cggggccact cggtcggtca
    11401 gtagttctcc cagtatccct tgtggtagtg gtggaaccgg tcgtagtacg gcttgtgcca
    11461 gcggcgccgg gggggccgct ggaaggcgat cttgctgaat gcgctcatct gcgtacatcc
    11521 tttcacttca tctcgaacgc cgtgtggcgt tctaactcta gaacgtacct gaagtcaggt
    11581 cggttcagga ctcgagccgg tattacccaa acggagtagt gattttgcga gccttggggc
    11641 ggtcatgtta cgccgaactt tccggccctg actgtccatg acgtatgaac tcgattatta
    11701 aagagatggt cgagtggcgc ttcgacgaac gccaaatcag gatatgcgca tcgctgccca
    11761 ccaggcgatc ttgtgcagcg gtcggtgacg gtcctcggca tcgcggccgg ggtcctgtgg
    11821 ccctggtagc acccgggtcg tgatgaatcg cgcggaccgg gtggatcgcg cggacgggat
    11881 gatcaggtcg ggatgccgag ctctgcacgt ccgcaggagc gcggcacacc tgtcagcggg
    11941 caacggcacg ccgtcgccga ggcgtgggcc cggttcgccc tccgtcgcca gaggtccgca
    12001 tcaccggcca gaaccgcccg accgggaggt ccgatcgagc ggttacggac gccggcgtca
    12061 gatggccgcg gaccgtcggc tacctcggtg taggcggggg tgcggggctg ccggcgagag
    12121 cgggcaggaa ctcgggcacg gtggggcgga gatgccggga ggcgacggcg gctgcggtgg
    12181 cgagggctcg ccgggcagcg tgctgcccat gggcagcaca gcgtcacagg tccggacggc
    12241 agcgcggtac gtcgatgaga gcgggtcgat gcgcgtgccc gtcagggtcg gccgagatcc
    12301 ggcgcacggt tgccgtcatc ggcacgaggg gacgcagcac ggccaccagc cacccgagca
    12361 cagcagggcc tcgcccgccc gtgcggcggt gcgggtgagc aacagcggtc tggtgatcct
    12421 tcccgatcag gtcgacggcc tcctcgagcc ctttcgccgc ctgggacgga ccggcgggcc
    12481 ggcgatcgcg gccttggcct tggcctcggc ctgtcgatcg tggccgcgat cgccgaggcc
    12541 cacaacaact ctcgaccacc ccagcacctc aggggaggtg ctgctccgga gacccgagag
    12601 acgtgtcaca agcccgcgac acacctcaga tgaaggcgga tcctcgtcgg gacggtggac
    12661 accgagagga gacgaggacg tggacacagc tccgcagcgc cctgatgtcc cgctcgtccg
    12721 ccggtaccac gtcgacctgt gccgcacgag caccgcggtc tgtccgcgct gaccgtccgt
    12781 ccctgcacag gtccgcatcc cgcgcggcgt tccgctcctc atagtccggc gagccgcccg
    12841 cacgcacgcc ctccgaagga aagacatgtc cgtcgagacg ccagctcgtc ccggctcctc
    12901 ccgatccctc ctgtcccccg ccccgacctg tgcgcccccg ccggcgcccg ggcccgcccc
    12961 gtccgtgcgg tgggcgccgc tcgttgcttt cgtcgtggtg gggatcgcgc tcacagccgt
    13021 ggtcgccacc gggtacggcc tccggcaggc ggtcctgctg ctgctcgggc tcggcctcgg
    13081 cttcgcactc ctccactccc gcttcgggtt cacgtcggcc tggcgccagc tcgtcgcggt
    13141 cggcaacgga gccggcctcc gcgcgcacgc gctgttgctg ggcaccaccg cgaccctgtt
    13201 cgcgctggtg atcgggaccg gcgcgggcct gttcggctcc accccggcac cgtcggccgg
    13261 cccgctcggc atcgcactgg tcttcggggc gttcctgttc gggatgggca tgcagctggg
    13321 cggcgcctgt gcttcgggaa cgctgttcgc ggtcgggtcc ggccagggga cgatcgtgct
    13381 cacgctgttc ggcttcgtgg ccggttcggt gctctacacc tgggcctacc cgctcgtcgc
    13441 cgatctgccc gccctgcccg cggtcgtgct ggccgagcac atcggctggt tcggttcgtg
    13501 ggcggtgacg atcgcggcac tggtcgccgt tgtgctcgtc agccgggtcg ttcaggcgcg
    13561 ccgcaacccg ccaccggtgg acgtggttcc gaccgcccgg ggcgcggccc gcatcgtccg
    13621 gggcgcgtgg cccatgctcg ccggggccgt cgtcctcgcg gtgctcggcg ccggagtgct
    13681 cgtcgtctcg ggcggggcgt ggggtatcac cagcgccttc ggcctctggg gggcgaagct
    13741 gctccagacg ctggggctgc acccggagac ctgggcgttc tggcagcagc ccgcacaggc
    13801 gaagatgctg gccggcccgg tgctggccga caagacctcg ctgaccgact tcgggatcat
    13861 gatcggcgcc gggatcgccg cggcatccgc gggtgcgtgg ttcctgcacc ggtcgatccc
    13921 gtggcgcacc gccctggccg ccgtgatcgg cggggtcatc atgggtgtcg gcgcccggct
    13981 cgcaaacggc tgcaacatcg gcgcctacct cgccggtatc gcgtcaggca gcctctcggg
    14041 ctgggtctgg gggctcgcgg cgctcgcggg gacgtgggtc ggactgcgcg ggcgtgccct
    14101 cttcgggctg agcactccgg ctccgtcgga ctcggtctgc tgatcgacca tcggggtcgg
    14161 cgacgtacag ccacgctcgc gtcgccgtgg gtcagtgctg cgcgcccagc tcgtgcaggc
    14221 ggcgatccag gaagcgccgc tccggctccg ccgtggccag ctcccgggct cgccggtagg
    14281 cgtccaccgc gtccgggatg cggcccaggc gccgcaggag gtcggcgcgg gtggcgtggg
    14341 cgaggtggtg gcgggcgagg tcaccgctcg cgagcaggcc gtcgacgagt tccacacccc
    14401 ggacgggatt cgacaggcgc ggcagatgac gttgcatcgt ggccacggca cctgctagtg
    14461 gttcttccgg ttctttcggt atcgggttcc ccaggtcgcg ctgcggactg tgtcggtcga
    14521 ttccaggccg gatccgaggg caccgccgat agtggccagc gaggagacca gccaggcgag
    14581 ccggacgtag tcggcgaagc tcaccggccg tccgatcgcc tgggagaaca cctccgggac
    14641 caccacgaac cagccggtca ggaaatttcc gacgagcagc accatgtatg acacgaggac
    14701 gccgatcgcg agagtcaaca gcgtcgctgc gttgtagaga cggatccgat ggcgttctcg
    14761 cgggtcgatg ccgctcgggt gctgccacag tccatggttc aagatcagcc agccgatgat
    14821 cgcggcgagg gagaccgccg tggcgatcca caggcggacc gtgctcatgg ccccgctgac
    14881 ctgccagacc ggggcggaga ccagcccgaa cgcagcggtg gccagggccg cggcgagcgc
    14941 accgcgcaac ccgagcacca gtcgccacgg ccggttcacc cgcaccatcc cgaccagcat
    15001 ccgcagctgc ccgcccgact tggtcagctg caggtcgatg tcatcgtcgg cgggctcgac
    15061 ccgatgcagg ctcgtcaccc gatcctcgag tcgctcaagg ccctcgtcag cgccaactcc
    15121 ggccgcccat tcgaggatca gcccggcgat gatgtcgcgg gcctgctccg ctgggttgag
    15181 caccttcaac gccggaacga cgagcaccgc ggctcccgcc tcccggctca gctcggccac
    15241 gacgggccgg ccgtccttgt gcaccggcag gtcggtcaag cagatcgcga tgtcccagcc
    15301 ttcccgcgcg cgccgcttgg ccgccacgtc gatcagccgt tccccgcggc tgtccccgtg
    15361 gcgccgcagc gtctccgaga ccaccgtgaa cgtgtagcgc attccgtccc ggggctggtc
    15421 ggccagcatg ttcggcagct cctcggcgac ctgctccgcg atctcggtag gaagcccggg
    15481 atcggccagc agcccgacgc gcgcgttccg ctcctggctg tcctcggcct cccaccggat
    15541 cggcctgtcg agcaccttct gcgatggcat cgctgccccc ttccgggccg ggtcggcctc
    15601 atcggccggt gctgtccgtg cgcgccggag cgccgactcg cccggcgacc aggccaacct
    15661 cggccgatgg gcaggtttct gtcctcgacc cgaggaacgg agagcatgac ccgctcaacg
    15721 ttcatcggtt tgcggctgat cggcattgtg gcgtgaaatc acgctctgcg ggccgctgag
    15781 ccctccccga gggtcgaccg cgaagtggcg gatcatgcgg cgggtggagt gccgcacctt
    15841 ctcgaccgtg gtgccgtcgt cctgcgcggc gacccgccga gcccgctcca accaggagaa
    15901 gaacgcgtcg aggtgggcga cgtcccactc gacgagccgc agttcgccga gctgagggag
    15961 cacctgattc ttgacacagc tcgcgtaggc gtcggctgtc gagtccgccc ggcgctcctt
    16021 gatcgtgccc atccagatgt cggcagcctg ccggaaccgc gagttggact tgagcatccc
    16081 ggtccgctca ccacgacgac cgcgcagctc ctcctgtagc gcctgctggg cagccgttcg
    16141 ggacgtgccc cagtgctcga cccgacgcgt cacaccctcc ggatcgcgga agcggcagcg
    16201 ggcgaccgtc gtcgcggacg accttgatcg acccgtggtg gccgagagca aggggtggac
    16261 gggccatggc ggcagctccg gagagggttt ccgacaggtt tgatctccag aaacgctgac
    16321 agaggtcagg gacacgtccc tgacctctgt gtttggtgcg ccgcgaggga atcgaacccc
    16381 caacccgctg attaagagtc agctgctctg cctattgagc tagcggcgcc tgccatgacg
    16441 cccgaccgct cggcgccgag gaggaacgct acacgttcga cttacgggcc gtgcggccgg
    16501 ggccgcggca tggggtgagt gacgggactt gaacccgcga cttcctggac cacaaccagg
    16561 tgctctacca gctgagctac acccaccatg ggcccggcct cggccgggaa cctacatcag
    16621 cttagcgcgg cggttccgcg gtatcgaagc gggttgccac cggccctgcg acctgggccg
    16681 cggcggcctg ggcctcctcg gtcgtcggcc ccggctccgg gacgaacagc gtgcgccggt
    16741 agtactggag ctcgcgaatc gactcctcga tgtcggccaa tgcccggtgg gcgaggccct
    16801 tctccggttt cgcgtagaac acccggggga accagcgccg gcacagctcc ttgatcgagc
    16861 tgacgtcgac catccggtag tgcaggtggt tgtcgagctc gggcatgtct cgggacagga
    16921 agccgcggtc ggtggcgatc gagttgccgg cgagcggggc ggtgcgcggc tccgggacgt
    16981 gctcacggac gtagctcagc gcgagggcct cggcctcggc caccgtgacc gtggacgcac
    17041 gtacggcctc ggtcagcccg gagtgggcgt gcatctcgcg cacgacgtcg ggcatcccgg
    17101 ccagcgccga ctcatcggca tggatgacga cgtcgacacc gcgcccgagc acgttgaggt
    17161 cgccatcggt caccaacacg gcgatctcga tgagggcgtc acgctggaga tccaggccgg
    17221 tcatctcgca gtcgatccaa accagacggt cgttcacccc cgtcacatta ggcccgtcgc
    17281 cgtcgccgtc acccgcacta cagggtgagt cgcgctgcat ccgtgccgga cggggacggt
    17341 ccagctaggc tccggacccg tggagaacac catcggcgct gatcagagct ctgcagcacg
    17401 ggagatcgct gccgggtacg ccactgaggg ggcggccctc gagctgggcg cggtcgtcgt
    17461 cgacggggtg gtcgacccgg aagcgcgggt gcgcatcccg ttcgccacgc tcaaccggca
    17521 cggcctggtc gcgggcgcga ccggcaccgg taagaccaag actctgcagg ggatcgccga
    17581 gcagctgtcc gctgccggag tcccggtgct cctggccgac atcaagggcg acgtctcggg
    17641 gatggcgagg cccggcgagc ccggcccgaa gatcgagtcg cgggcccggg acaccgggga
    17701 cgactggacg cccaccgcgt accccgtcga gttcctgtcg ctgggcggca ccagctcggc
    17761 cgtcccgatc cgggcgacgc tcacccagtt cgggccgatc ctgctgtcga aggtgctcga
    17821 cctcaacgac acgcaggagt cgacgctcgg gctgatcttc cactgggccg accagcgcgg
    17881 cctgccgctg ctggacacca aggatctgcg tgcggtcgtc cagcatctga ccagcggcga
    17941 ggggaaggcc gacctcaagg gcatcggcgg ggtctcgccg gcgacggcag gagtgatcct
    18001 cagggcgctg gtgaacctcg aggcccaggg tggggaggac ttcttcggcg agccggagtt
    18061 cgagcccgcc gacctcatcc ggcaggtcga cggcaagggc gtcgtcacgc tgctggagct
    18121 gtccgaccag gcggccaacc cacgcctgta ctcgacgttt ctcatgtggc tgctcgccga
    18181 gctgttcgag gagctccccg aggcggggga cttggacaag cccaagctcg tgttcttctt
    18241 cgacgaggcg cacctgctgt tcaccgatgc gtccaaggcg ttcctggagc gaatcgagca
    18301 gacggtgaag ctgatccgct ccaagggcgt gggcgtgttc ttctgcaccc aactgcccac
    18361 cgacgtcccg aatgcggtgc tctcccagct cggcgcgcgg atccagcacg ccctgcgggc
    18421 gttcaccccc aacgaccagg ccgcgctggc caagaccgtg cggacctacc cgaacacgaa
    18481 gacctacgac atggaggagg cgctcaccac gctgggcacc ggtgaggcga tcgtcacggt
    18541 gctctccgag cggggcgccc cgaccccggt cgcctgggcc cggatgcggg cgccgcgctc
    18601 gctgatggcc gcgatcggcg ccgacgccgt cactgctgcg gcgaaggcgt cgcccctgta
    18661 cggcaagtac gggcagacgg ttgaccggga gtcggcctac gagtcgctga cggcgaagat
    18721 cgccgcgacc cccggccccg aggccgcgcc ggccgatgtc ccaccgccac tccgggagcc
    18781 cgagcggcgc cgggagcgtg atgagccctc gatggtggcc gaggtgctcg cctcgccggt
    18841 cgtcaagtcg ttcctccgat ccgcggcgag cgcgctgggc cgggagatca cccgcggcat
    18901 gttcggcacc cgccggagac gctgacccgg ggcggtcgct gttccggaac tccatcgacg
    18961 agcgcgcagc ggatctgcgt gtcggccggt agcaggtcga tcaacggtgg tcgagctgcg
    19021 gctcctccgg cgtggctgcc gcgaggaagg gcgtgagcag gtgcggaacg gccgcgtcgg
    19081 cgagcgccac gccgtctgcg gccgcgatgt cgagcacctc gtagccgccc gcgccggccg
    19141 ccgtgatcgc ctccaccgcc accagccccg cacgacgctg gaagaggctc tgccggaacg
    19201 tccagccgat cacccccgcc cgctgcaggg cgacggtccg ccgctgtacc gagccatggc
    19261 ggctcacgag gtagcgggtc gtgagctcgt ggcccaggtt ccggtagcgg tcgagagcga
    19321 ccaggccggc gagcgggagc agcagcaggc tcggcagccc cagcccggcc aggacgggca
    19381 cggccgcccc ggcggcgaac acgccggcca ccagcacggc ggtgggcgcc agtgcccggg
    19441 tcaggcgtcg tcgcagcgcc gcgcgcgggt gccggcgcag cggcgcgagg gtgacctcgt
    19501 gtcggtgctc gcgcagcgct gccgcggcca cgcggtgcgc ctccgcgcgc gggcagggcg
    19561 gctgcaacgc cccgccctgg gcggagcggg tgaggccggt ggacagcgcc ttggagtagg
    19621 cgccgtgccc ggcccgcagc aacagcggct cgacaacctc cacgccccgc agccgctgct
    19681 cggacacgga gagcgagcgc cgcgtgagta gccctcgtcg gacgcgcagc gtgccggtac
    19741 cgataccggt ggaggcggtc gcccccgccg gatcccaccc gggaccgtcc gcggccgctt
    19801 cacgggtgag ctggtagccg aaccagcgct cggcgaacag caccatggcg ccgatcacgg
    19861 cgaccaccag caggagcgcg gccagcaccg cgacggcgac ccagatcggg gtggtggtca
    19921 gccgatcggc ggcgtcgtcg acggccgaga tgtcgcgggg gctcacgccg agctcgccga
    19981 acaggttgaa caccgccgcg ccgatcgcgc cgacgccggc gatcgcggag aacgtcaggg
    20041 gtgcgaagcg caaccaggac cagcgcagcc gggccagctc cgtcgcgggc gggggcgcgg
    20101 ggtggttcgc ggtaggcccg gtcgcggtcg gcccggacgc gatgagcgag cggtccagca
    20161 gttcgcggcg cagccggtct gcctccgcct tgctgaccgc gtcgagggtc agccccgcgt
    20221 tctccgtcgg ggacccggat gctgccccga tgtgcacgac actcagcccg aagatgcggt
    20281 gcagcagctt cgcggtgagg tccaccgtgc ggatccggtc gcgcggtacg gatcgccgct
    20341 ggcggcgcag ccaccccgtg tgcagctcga cccgatcgcc ggtgatccgg tactgcgtgg
    20401 tccgccagcg caccacgcca ccgatgacga ccagcacgcc gatcccgagg gggatccaca
    20461 ggcgcatcgg gtcgcctcgc tgtcccgtga cgagcaggat caccgcgacc ggaagcaacc
    20521 gcaccaggcc tgcgatcggg ctcaccacga gcatgcgctt gtcgagccgt cgccacggcg
    20581 tctcgatggt ggtcggtgcg tcggtgtcgg acgtcatgtg gcgtcgccag gcgtggcctg
    20641 ggtggtttcg gtgagcgtgc gggccagctc ggcagcggcc tgggcctcca gaccgctgat
    20701 cttgaccggg ccgcgcgccg aggccgtggt gacagtgacg ctggccagct tgagcagctg
    20761 ctgtagcggg ccgtgctcgg tgtcgaccgt ctgcacccgg gagatggggg cgatccgcca
    20821 ctcccggacg agccagcccg acagcgtgta gacggcctcg ggggtgacct cccagcggtg
    20881 gatccggtac agcagcggag gcacgacgat cgcgtacacc agcgtgaccg cggcggtcgc
    20941 tgccgcggtg taggcgagcc agcccggtgc gcgcgagccg agcacgagca gcacgacgac
    21001 ctgcggcgcg acgaggacca ccaacgtcag gagtgcccgc acctgccacc accgcacggc
    21061 ccgggggctc acctggtggg cgggcgggcg gagcgggagt gcaggcgtgg tggcgatggc
    21121 catgcccgga gtctgcccgt gaccgccttc accgtgcgat ccggatgcgg acgggttctg
    21181 cgtcatcggg ggtgggcgaa cgccgccagc tgggcttgcc gtactccagc cgtctgggcc
    21241 tggatcaggt ggaacgccgc ttcccgggcc caccgctgtt cggggctggc caggagcaac
    21301 gcgctgcccg agcgggccgc gatcagcgcg ttcgccgtcc ggacggccag ctccgtgacc
    21361 tcggcccgca gcgccgtccg ctcgggcacc cgctcgacca acggcacggt ggtcagtagc
    21421 gcgtacgcct cggtccggcg gtccgcggcg cgttcggcga gggtcatggc gaggtcgacg
    21481 gcctccggcc gggccttcgc cgtgccgagc tcggcgagag cgacgagcat gcgtcggagc
    21541 agtccgaggg acgcgggcgt ggtgttcgcg gtgcgggccg cgtcgtgcgc acgccacgca
    21601 cggacgtcca ccgtggcgag cacgtcctcg gcgtcgatct gcatgccgtc catctcgagc
    21661 gcgacggtgc gcgtgccgcc catgacggcc agcggcagcc gcggcccggg tcgcagaccg
    21721 ggacgttccg tggcgggcag cagggcaagg acgaaccggt cgtcggtggt ggacgccgcg
    21781 atcatgacga cctcgatcag gccccagccg gtgcaccagt cggcggtccc ggagaacgtc
    21841 cagccgccgg gtcgggcctc ggcccgtacg gcgggcggcc ccggccgtcg gatgtgggcg
    21901 atcgcgatac cggcgctcgt ggtcgccgcg gccagcccgg cacggtggcg ggccgcagcg
    21961 ggacccagct cgatggcgcc agcgtcgagc ccggggagcg gaccgcggga cagaccctgc
    22021 gggaagcggt gctgggtggt gaggaaccag gtggccccac atgctccgct gaccagctcg
    22081 acggtctcgg cgtccacccg gtcgtcggcg ccgtgcccgc cctccgcggc cgggatcttc
    22141 accgacagca ggccgtgttc cgccagcagc ctgagatgcg ccggatcgac tccacgggcc
    22201 gggtcgtcgg ccgcctcggc gtgttcggcg agcacatcgg ccgcaatctg acgcgcggcc
    22261 cgtacggcag gatgctcggc gacccgcggt tcgggtgcga acagcgcctc gacggacgcg
    22321 gccgcagcgg acatcacggg cccggacgtt acgcgaggcg gcgcagcccg atcgcccgca
    22381 gcgcggcgta gggcaccgcg tcggcgccac aggacgccgg ccgcaggccg ggcccgtcca
    22441 ccgcggcaac gtcgacggcc cagcgcccgc cgtcccgcgc ggtgacgatc gcgcctccgc
    22501 tcacgtcgga aacctgcagc gcgtgggcat cgcgcagccc ggtcgccgcg cgcaccgcca
    22561 gctcggccac ctggccggca ggtgaccacg tcgaccggcc gcggcacaac cccgtctcga
    22621 cctcgcccga gctcacggcc ttgtgcgccg ccaccgcccc cgccgggtcg agccgcccgt
    22681 aggtgtagcc ggtgggaagc acgagcgcgg tcggggcgaa gcggtgcccg ccgaggtggc
    22741 tgcactccca gacgtcgagc tcgctcccgg cggccacggc cagtgcgagc gcgcgtccgt
    22801 cgagtgcgca gcagcggtcg cgcctgccgt gggtgcacac cagcagcagg ggctcggcga
    22861 ccacggcgcc gggcagcaca tcgcccaccg cgggcagcgc caggtcggcg agctccgacg
    22921 gtccggccac catgagcctc gcggtgcggg caatgggcgg agcggtgtcg gtgatgtaga
    22981 cccgcgtggg accgcccgtc tcgtcgcacc gccggcccgg acgccggatc agtagcaggc
    23041 gccaaccagc ggcgcgggcg cgcgcggcga aggtggcgat ggccgggtcg gggtggtcct
    23101 cgacatcccg tggccacgct cctcggtgct ccacgcacac ccagcggggt gcgaccggtg
    23161 ccgtgccgac cagcggctcg tcgagggcgt gggcgagcag ggcgcaacgc gcaccggccg
    23221 ccgtctccgc atccaccgcc gcacgatatc cacggctgcc ctaactcagg cgagtgatac
    23281 tgctcatctc cggtcggggc tccgcagctc gcggtgcacg accttgccgg tggcgttgcg
    23341 ggggagctcc tcgacgaact ccacgtcgcg cggcacggag aagcgcccga gctcgtcccg
    23401 tacccaccca cgggcgtcgt cggggtcgac cgaggtgccg ggctcgagca ccaggtacgc
    23461 ggccagtcgc tggccgtact gctcgtcggg cacgcccgtc acggccgcct cccggacctc
    23521 gggccgggcg gcgagcagat cctccaccgg tcccggatgc acgttctcac ccccggagac
    23581 gatcatgtcg tcggcccggc cggtgatgag cagcctgccg tcctcgtcga gcctgccgat
    23641 gtcgccggtg ccgagcagcc ggccctcgcg ttcgacgtcg gcgccctcgc gggtgtagcc
    23701 ctcgaacggc atgtcgttgc gcacgaagat gcgcccctcg gtgcccgggg gcaccggacg
    23761 gccattgtcg tcgaggacga ccagccgggt gccgggcggg gcgcggcccg cggtgcccgg
    23821 tgcggcgcgc aggtcggccg gggaggcgat gctcacccag gacacctcgg tggagccgta
    23881 gaggttgtag agcacgtcac cgaagtgatc caggaatgcc gccgccacct ggggtggcag
    23941 ggccgaaccg ctgacggcca cgacccgcgg ccggtgccgc gaccacgcag cgtcgagttg
    24001 ggcggcgggc agctccagca gccgttgcag catcaccggc accgcgaaca cggcgtcgca
    24061 gcgctgctcc tcgaccacgc gcaggaactc cgccgggtcg aaccggcgcc gcagcaccac
    24121 cggggccccg tgcagcacgg ccagctgcaa cgcggcggat ccccaggtgt gcagcagcgg
    24181 cgccgcgatc agcaccggtt cggcggcgcg gagcgggatc gtcgacagga tcgccgccgc
    24241 cggggcgagg ctgtgcggcg ggggccggcg ggcgcccttc ggggtgccgg tcgtgccgga
    24301 cgtcagaacg atggtgcggc ctgcgggggg tcgccacggc agcgggccgg gaccgtcgag
    24361 ctccgtcagg tcgtggacga ccgtgcatcc gtcagggagc cccttcagcg ccgcgtcgaa
    24421 ctccgggtcg gcgacgacgg tgtgcagctg cagctcgacc gcgatctcgc tcagctggcc
    24481 ggctgagagt ccggtgttga gcagcaccac gtcggcgccc agtttgccca cggccacctg
    24541 cgtctcgacc gcgccgcggt ggttgcggca cagcagcccg acgcgcaccc ccggaccgac
    24601 gcccgcagcg gcgaggcccc gcgccatcgc gtctgtgcgc cggtgcagct cggcgtaggt
    24661 gacggcaccg tcgtcgtcca cgatggccgt gcggtcggga tggcgcgcgg cgcccacgac
    24721 gtagccggcc gcgatactga acccgtagcg cagcaggccc tcgccgattc cgaggagccg
    24781 gtccgggcgc tccacgcgga gcaccccgga gcgcgtgagc gcgacgaccg cccgggccga
    24841 cccgaccgcc tgctcagcac cggaccggac ggttccgagc acccgcgtga gcacatcgat
    24901 cacgcagacc agtgtcgcag cggctgcgcc tgcttcgcag tccggagacc gactgcggct
    24961 tcgatcacac ggcgacagcg cccccggtga tcgccgtgag ccgcacacca gtgcccgggc
    25021 gagggccgta gcgtggggat cgggccgatc atggggcgag tggaaccgca ctctcccggt
    25081 gtgatcacca cgagccgcac gcgggcgccc ttcgcgggga gctggtgtac ggctcgcgac
    25141 gatcttggcc ggatggtcag atgcgggcct gcgtcaggtc gtagaccgtc tggccgccga
    25201 ccgtgacgtg cgtgaagttg tcctccaccc atgtcgagat caaggatccc gcgcgaccgc
    25261 cctcgaaccg gtccgccacg aagtagtgca cctcgcccgc gcggacgtag ctctggaact
    25321 gctccagggt gggagcgggg tcgaaaccgg tgaaaccgcc gatcgccatc accgtcgttc
    25381 cgctggacag ggcgagctgt gcgctgcccg acgccccaac cgtggctgcg gaccacttcg
    25441 tgcccgcctg ctggaggagg gcgacgagct cgggatcgat gttctgctgg gggcccccga
    25501 gcatggcggc gacccccagg gggaggccat gggggtttcc tcggagcctg tcgaggaaga
    25561 ccttcgtggc atcggtcggc ggacccgccg ccgggatgct gccggtgtgc ggggtcgccg
    25621 ccgtctccac ggcgaaggcg gcgggcgcgg cgaagcccgt gagggccacc acgagcgcgg
    25681 tgacggcaac ccagcgccgt ccggcgggcc gcaccagcaa cgcgagcacg gcgagtgcac
    25741 ccacaccgat cacgacccac cgcagccagg gcaggaactc cggggaccgg tcgagcagca
    25801 ccaccgccca cgccgccgtc ccggccacga ccagcgcgag tgcgacccgg cccggccacc
    25861 gcgcccggtg gcgccacagc tcgtgtccgc cgacgccgag cagcgccgcg atccccggag
    25921 ccagcgcgat cgtgtagtac gagtggaaga tcccctcggc gaggctgaag accagtgcgg
    25981 tcacgatcgt ccaggtgccc cacagcagga gggaggcgcg ggtgcggtcg atgcgggggg
    26041 cgcgccacgt gagcccgagc ccgatcacca gcagcgccaa tgcggccggc agcagccacg
    26101 acacctgtgc gcccacgtcg gcgccgaaca tgcgcgtgag cccggccggg ccgccgaagc
    26161 cgccgacacc acctccgccg tggccgccca tcctgggatc cccggaaccg ccgaagatcc
    26221 ggccgagtcc gttgtaaccg aacgcgagct cgagcacgtt gttggtctgc gagccgccga
    26281 tgtacgggcg gtcggccgcg ggccacagct cgacggtcgc cacccaccag ccggctgcga
    26341 cgaccagggc gatcccccct gcgaggagct ggccaatccg gcgccagaac ccggtcggtg
    26401 ccgccacgag ataggcgagc gctagcgcgg gaaccacgat gagggcctgt cccatcttgg
    26461 ccaggaacgc gagtccgagc agcgcgcccg cgagcagcaa ccagcgggtg gcggcggcct
    26521 ccgtggcgcg gacggtggcg tacgccgcgg ccaccatcag tagcacgagc agcgcgtcgg
    26581 ggttgttgaa ccggaacatc agcaccgcga cgggcgtcag cgccatcgcg gcgccggcga
    26641 gcagcccggc gcgcggtccg gacacccggc gcaccgcggc gtacatcagc gcgaccgtgg
    26701 cgacgcccat caacgcctcc ggcaccagca tgctccagga ggagaacccg aagatccgcc
    26761 ccgagagcgc catcacccag agcgacgcag gcggcttgtc gacggtgatg atgttgccgg
    26821 tatcgaacga gccgaagagg aacgactccc agctgcgcgt catcgactgc acggccgcgg
    26881 cgtagtagct gttggcgtag ccggacgcgc cgagggccca caggtacagc gctgcggtac
    26941 ccgccagcag cgctgccagc gcgagccgct cgtaccgcct cctgggcggc ggtgcgaccg
    27001 tgaccggggc cgggccggtg ggggcgagcg tggcggtcat ggctggacct cgtggacggc
    27061 gatcgtgatc cggaaccggg acggggaccg ctcgctcagg tgggcgtgca gccggcggac
    27121 cggcggctcg aggtcggact cctcgttgaa cgcggggacg gcgacgtcga gcacgccgag
    27181 gggcggttgt ccggcggtgg tcggatgcag cgcggtatcg acgctcattg catcgaacgt
    27241 ctatggacgc gctgtccgaa ccctgtgccg atgatgtgaa cgggctgtgc gtcccacgga
    27301 cccgaggtga cgatggtcat tccgctcagc ggagctgcgg ccggggtggc gtcggctcgg
    27361 ttcccgggga gcctgtgggg tcgaggtgca gcacgcgcgt cggcatccgc acaggtggat
    27421 cgctcgggag gtggacggtg aacgcggtcc gaccaggggc gctcgccacc ctcaccgccc
    27481 cgccgtgcgc ggtcacgaca gcgtcgacga tggcgagccc gagcccggtg ctgccgttcg
    27541 cccgtgatct cgaggtggag ccccgcgcga accgtttgaa cacgtggggc agcagctcgg
    27601 gcgggacgcc gggcccgtta tccaccacgt ccagccgggc ggccgtgggt tccacggcga
    27661 gggcgacggt caccgcggtg cccgtcgggg tgtgggtacg ggcgttggcc agcaggttgg
    27721 ccagcacctg ggcgatccgc aacgggtccc ccggtaccgt cacgggctcc tcggggagcg
    27781 cgagctgcca gcggtggtcg gggcccgccg cgtgcgcgtc gctcactgcg tccacgacga
    27841 gccgggtcag gtcgacctcg gcgcgctcca gagggcgccc cgcgtcgagc cgcgcaagca
    27901 ggaggaggtc ctcgacgagc gtggtcatcc gctcggactg ggcggagatc cgcgcaaggg
    27961 cgtacgcggc ctcgtccgac agctccccgc gcctgctcag ctcggcgtac ccgcggatgg
    28021 cggcgagcgg cgtgcgcagt tcgtggctgg catcggcaac gaactggcgt aaccgcatct
    28081 cgctgtcctg acgggcctcc agcgccgcgc cgacgtggtc gagcatgtgg ttgagtgccg
    28141 cgccgacctg gcccacctcg gtgcgcggat cagcggcggg cacccgctcg acgagcgaga
    28201 cctcaccccg gtccagtggc agcgtgctca ctcgcgctgc ggtggccgcc acccgttcca
    28261 gtggccgcag ctcgcgccgc accagtgcaa cgccggcgag tccggcgccc agcagcgtca
    28321 ccgtggcgac gatcagctcg gtcgtgacga gtcgggcgac ggtgtcccgc accggacgct
    28381 cggtgaaacc ggtgacgaag acctcgccgt tcccgcccga cacggccacg aggcggtagg
    28441 cgcccatggg cccgaggtcg gcgctcagtg gcggccggtc cacagtgatg gaggtcagat
    28501 cggggtactc cgacgggggt acgggctgta cgccaccgtc gcgggtgaac acccacgcgg
    28561 agacgacgct gccgtcccgg agcacggcgg tcaggctgtc gggcggccgt gggcccagcc
    28621 ggccgcccgc gtcgggcggc aggagcggat ccaacgggcc aaggcgggtg agcactctgc
    28681 tcgacgcccg caggtcaccg tcgagcctgc tgatcaggaa gccccgcagg gtgagggccg
    28741 tgaccaacgc gaccacggtc gacgcgacgg cgagtagcag cagcagcatc gcgacgagcc
    28801 gtgtccgcag cgagcggctc ctcacccccc ggccgccggt ttgagcacgt agccggcacc
    28861 gcgcacggtg tgaatcatcg gcgcccgccc ggcgtcgatc ttcttgcgca ggtacgagac
    28921 gtacagctcg acgacgttgg actggccgcc gaagtcgtag ttccacaccc ggtccagtat
    28981 ctgccccttg gacagcacgc gcttcgggtt gcgcatgagg taacgcagca gctcgaactc
    29041 cgtggccgtc agctcgaggg gcgcgtcgcc ccggcgcacc tcgtggctgt cctcgtcgag
    29101 cacgaggtcg ccgacgacca gctcggagcc ctgcctcgcc gccgacatcc cggaccggcg
    29161 cagcagtcca cgcagccggg cgaccagctc ctccaggctg aagggcttcg tgacgtagtc
    29221 gtcaccgccc gcggtgagcc cggcgatccg gtcctcgacg gcgtccctcg ccgtgaggaa
    29281 cagcaccggt acgtcagggg tctccgcgcg catcctgcgc aggacctcga gaccatcgaa
    29341 gtcggggagc atcacgtcga gcacgacggc gtccggccgg aactcccggg ccgcgcggac
    29401 ggcggagccc ccgtcggccg cggagcggac gtcccagccc tcgtaccgca gggccatcga
    29461 gagcaggtcc gacagcgagg cttcgtcgtc gacgaccagg atccggatcg cgcttccgtc
    29521 ggcccgccgc aatgccgtgg cctggtccct catcgtcgac atgctagcca gcctcgcgcg
    29581 cgccactgtg ccgtggatga gcgaatcctg tgcgtccgct gtggaaccgc agcgcgtgct
    29641 ccgtcgtcca agttcgcatc ccaccgcacg cgaggagacg acatggacac gttcgaaggc
    29701 tggcccgccg acgccacgac attcctggcc gagatcgccg ccgacaacac ccgcgagttc
    29761 tgggccgagc accggcaccg gcatgccacc tctgtgcact cgccgatgcg ggcgctcgcc
    29821 gccgagctgg agccggagtt cgggccggtc cgggtgctgc ggccgttcgt caacctgcgg
    29881 ttccggccgg atgcctcgcc ctaccgcacc gacgccggcg gtgtggccac cacggcgggg
    29941 ggctgcccgc tcgccgtcgt cgtgtcggca gcggggctgt cggtgtcggc cgggcaccgg
    30001 tcgttcgacg ccgggcagct ccgccggtac cgcgccgccc tcgacactcc ggcgggtgcc
    30061 gagctcgccc agctgctcgc cgagctggac gggtacgccg tcgagcccgg ccgtcccctc
    30121 accggcatcc cgcgcgggtt ccgtgccgac cacccccgca tcgcgctgct gcgccgccgc
    30181 ggggtgcagc tcgtacgttc gcggccggcg ggggagtggc tcgcgactcg cgagcccctg
    30241 gattgggtcc gggtggcgtg gcgggcggcc ggcccggtcg tcgcctggct ggacctgcac
    30301 gtgggtgcgg ccgagcgggt gccgctgccc cgacgagtac ccggtcccgg ggtcgcagat
    30361 agtagcacgg acaccatgaa atcggtcatc gtcgagtcgg ccgaccggta gcgggggctt
    30421 cagcgggcgc gccagtagcc cgtcgcgtag accttgcggc ggtccaggcc gcggtcgtcg
    30481 agcaggtgct tgcgcaggtc acgcacgcag gccgactcgc ccgagagcca cgcctgtccc
    30541 gcccctgccg gcagctcggt cgaccgcacg gccgcggtca gcggcacgcc cggttcccgg
    30601 tcgccgcggt ggacccagcg gatcgtggcc gctcccggca gctgctgctc ctcgtcggcg
    30661 tcggccacct cgaggaaggc gagcgccggc acaccggcgg ggagggcgtc gagcaccgtg
    30721 gcgacggcgg ggagcgccgt ctcgtcgccg atcacgatgt ggaagtccgc cgccgggtcc
    30781 ggctcgtacc ggccgccggg ctcggacacg cccacccagg agccggggca cgcaccccgc
    30841 gcccacgcgg ccgcgggtcc gtgcccgtcg tgcagcacga agtcgacgtc cagctcaccc
    30901 gcggccgcgt cgaaccgcct gacggtgtac gtccggatcg ccgggcgggg ctcgcccgcc
    30961 ggccacagtg gcccgcccga tgtcgcccgg ggcaccgcag gccgatcctg ccctggcacg
    31021 gggaagaaca tcttgatgcg acggtccggt ccgtcgccgc cgaagccggc gagctcgtcg
    31081 ccgccgagcg tcacgcggat catccgtggg gagatccgct cgctgcgtcg cacctgcagc
    31141 agccggggtg ccgtcgtcgt catcgcgacg attgtgccgc tgccccgccc gcggcagcga
    31201 ggcccgtcca cacgaggtcc atcaggtact ccgtggcctg ctcgctggtg accgtccgct
    31261 cgcggccccg ccacagcgcc agtcgctcgc acgctccgac gatcacctgc gcccagccgg
    31321 ccagccgttg cgggtcggcg tcgggtgcct gcacggcgag cagggcggcg atgaagtcgg
    31381 tctgctgggc ccggatgccc tccagcgcct cgcccgccac cgtcgactcc gagtacagga
    31441 gcgtccaggc aggctcgcgt cgctcgaggt actcgaagaa ggcgagcgtg ccgcgccgca
    31501 gcatctcctc cgggccgtcc gccgatgccg cagccgtggt ggtcacctcg agcagctcgg
    31561 cgcgggcgcg ggcgatgcac gcgagcagca gcccgtcctt ggacccgaag tgggcataga
    31621 gcaccggctt cgtcacgccc acccgctcgg ccacgacgtc catggaggcc gcctggtaac
    31681 cgcgctcgcc gaacaccgcc acggccgcgt ccaggatctg ccgctcgcgc tcggcgcgac
    31741 tcacgcgccg gcggggcgtc ctggtgtcca tgccgccaac ctaccgcagg taactcgaat
    31801 ctaccggcag taacctactc gtggtaactt cgtggcgatg ccaacggagg gaacaggatg
    31861 agcgagccga ccgccgatcg cagcgccgac gtcgcggatg ccgttgtgga tatcgccatc
    31921 gtgggcagcg gcttcgccgg cctcggcgcc gctgtccggc tcgcacaggc gggccgcaac
    31981 gacttcgtca tcctggagaa gggcgagagc ctcggcggca cctggcgtga caacacctac
    32041 ccgggttgcg cgtgcgacgt ccagtcgcac ctgtactcgt tctccttcgc gcccaacccg
    32101 aactggaccc gcctgttcgc gcaacagccc gagatccgcg cctatctcga ggacatcgcc
    32161 gaccgctacg gcctgcgcgc ccgcatccgc ttccgctcca cggtggtcgc cgcggagtgg
    32221 ggtggcaggc actggaccgt gcggatggcg gacgggaaga gcatcacggc ccgcgtcctg
    32281 gtctggggca cgggagcgtt gcacgagccg tcgatccccg acatcgcggg gctcgacgag
    32341 ttcgccggca cggtgttcca ttccgctcgc tggaaccacg accacgacct gcggggcatg
    32401 cgcgtggccg tgctcggcac gggcgcgagc gccatccagt tcgtgccggc gatccaaccg
    32461 ggcgtcgccc ggctcaccgt cttccagcgc accgcgccgt gggtgctgcc caagccggac
    32521 cgggcggtgt cgccgctgtg gcggcgggtc taccaggccg cgccggtcgt ccagaagctg
    32581 cagcgggccc tgatctacgc gcgcaacgag agcctcctga ccgggttccg caagccgcgc
    32641 cggatg
//
LOCUS       DASRSC010000293         2575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4048399,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000293 DASRSC010000000
VERSION     DASRSC010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4048399"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1284
                     /locus_tag="VFE39_14590"
     CDS             112..1284
                     /locus_tag="VFE39_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HET6259866.1"
                     /translation="MSTDDALEAALAAAGLAGATPEQTAGGGAIVVPGPVDQQDAAAA
                     EAAAARPRWPDGAATGVGSLPGTDPREAAAMVVGELPLLPHLPELPARGVGADMIGRT
                     AGLLVDIAVDFVPSGYRVTARPGGVHRRAVDLLRTDLDALDEACEVARPGWVKVQAAG
                     PWTLVAGVELHAGHRVLTDRGAVREFTASLQEGLRAHVTEVATRTGAHVLVQLDEPTL
                     PAVLAGTLPTASGYGTVRSVGRNEAQDALRDLVTGLGVPVVVHCCADRPPLRLLADIG
                     AAGIGIDATRPVFSGDTAQPAALDALGEIWDAGTPLLLGLVPTLDPGRPVTSRDLARP
                     AFDLADRLGFDRSRLAALAVPTPTCGLAGATPSWARQALALSRELGEAFLDPPENS"
     gene            1312..2412
                     /locus_tag="VFE39_14595"
     CDS             1312..2412
                     /locus_tag="VFE39_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HET6259867.1"
                     /translation="MHGDRLLLGPGPSTPYPEAVEALARPVLGHLDPEYLAVVHEIGD
                     RLREVFRTDNALTLPVSGTGSAGMEACLASLLEPGETVVVGVNGYFGERICEVAHRGG
                     ARVVRVEAPWGTALDPADVLAAQRAHPQAALVAVVAAETSTGVRNDVAPLGPALAQTD
                     TLLLVDAVTALGGTPLEVDAWQIDACYSASQKCLGAPSGLAPVTLGPRAVEKLRTRTT
                     PIHSFYLDLGLLHEYFLASPPRYHHTASSTLAYALHAGLGRLLEEGVEPVWERHARVG
                     ALLQSALPELGFTVVAPDGARLPQLTTAVLPDELDDALARTRLLADYGIEVGGGLGAY
                     AGRAWRIGLMGHAATERSVVTLLGATRELLKA"
BASE COUNT          299 a          975 c          946 g          355 t
ORIGIN      
        1 ggcccagagc ggtccggcgc gcagatccgt cggggcggct cgagtccact gcttcttcga
       61 cgcccggtac cggaaggcat gactgcgcac ggcgggtggt agacaccggc cgtgagtacc
      121 gatgacgccc tcgaggccgc gctcgccgcg gccggcctgg ccggggccac cccggagcag
      181 acggcgggcg gcggtgcgat cgtcgtgccc ggtccggtcg accagcagga cgctgcggcg
      241 gccgaggccg ccgcagcccg cccgcgatgg cccgacggag ccgccaccgg tgtcggctcg
      301 ctacccggca ccgacccgcg cgaggctgcc gccatggtcg tgggcgagct gcccctgctg
      361 ccgcacctgc ccgagctgcc ggcgcgtggc gtgggcgcgg acatgatcgg gcggacggcc
      421 gggctgctcg tggacatcgc cgtcgacttc gtgcccagcg gctaccgcgt caccgctcgg
      481 ccaggcggtg tccaccgccg ggccgtcgac ctgctgcgta ccgacctcga cgcgctcgac
      541 gaggcgtgcg aggtcgcccg cccgggctgg gtcaaggtgc aggccgcggg gccgtggacg
      601 ctcgtggccg gcgtggagct gcacgcgggg caccgggtgc tgaccgatcg cggtgcggtc
      661 cgcgagttca cggcgagcct gcaggagggg ctgcgcgcgc acgtcacaga ggtggccacc
      721 cgcaccggtg cccacgtgct ggtgcagctg gacgagccga ccctgccggc ggtgctggca
      781 ggcacgctgc cgacggcgtc gggctacggg accgtgcggt cggtggggcg gaacgaggcg
      841 caggacgcgc tgcgcgacct cgtcaccggt ctgggagtgc cggtggtggt gcactgctgc
      901 gcggaccggc cgcccctccg gctcctcgcc gacatcggcg ccgcagggat cgggatcgac
      961 gccacgcgcc cggtcttctc cggcgacacg gcgcagcctg ccgcgctcga cgcgctcggt
     1021 gagatctggg acgccggaac gccgctgttg ctggggctgg tgccgacgct cgaccccggg
     1081 cggcctgtga cgtcgcgtga ccttgcccgc cccgccttcg acctggcgga ccggctgggc
     1141 ttcgaccgat cccgcctcgc tgccctggcc gtccccactc cgacctgcgg tcttgccggt
     1201 gcgacgccct cgtgggcacg tcaggccctc gcgctgtccc gcgagctggg cgaagccttc
     1261 ctcgacccgc ccgagaactc ctgaccaccg tccgcgcgta ccgttcgacc catgcacggt
     1321 gaccgtcttc tgctcggccc gggaccatcg accccgtatc ccgaggccgt cgaggcgctg
     1381 gcccgccctg tgctcggcca cctcgacccc gagtacctcg cggtggtcca cgagatcggc
     1441 gaccgcctcc gcgaggtctt ccgcaccgac aacgcgctca cgctgcccgt cagcggcacc
     1501 ggctccgccg gcatggaggc ctgcctcgcg tcgcttctgg agcccgggga gaccgtggtc
     1561 gtcggcgtga acggctactt cggtgagcgg atctgcgagg tggcccaccg tgggggggcc
     1621 cgggtggtgc gggtcgaggc accgtggggc accgcgctcg acccggcgga cgtgctcgcc
     1681 gcgcagcggg cacacccgca ggccgcgctc gtggccgtcg tcgctgcgga gacgtccacg
     1741 ggggtgcgca acgacgtcgc tccgctcggg cccgcgctcg cgcagaccga cacgctgctg
     1801 ctcgtcgacg ccgtcaccgc actggggggc acccccctcg aggtcgacgc ctggcagatc
     1861 gacgcctgct actcggcctc ccagaaatgt ctcggcgcac cgtcaggcct cgccccggtg
     1921 acgctcgggc cgcgcgccgt cgagaagctg cgcacccgca ccaccccgat ccactccttc
     1981 tacctggacc tcggcctgct ccacgagtac ttcctggcga gcccgccgcg ctaccaccac
     2041 acggcgtcct cgacgctcgc gtacgcgctg cacgccgggc tgggacggct gctcgaagag
     2101 ggtgtggagc cggtctggga gcggcacgcg cgggtcggtg cgctgctgca gtcggcgctg
     2161 ccggagctgg gtttcacggt ggtcgcgccc gacggtgccc ggctgcccca gctcaccacc
     2221 gccgtgctgc ccgacgagct ggacgacgcg ctggcgcgca cgcgcctgct cgccgactac
     2281 gggatcgagg tgggaggcgg cctcggggcg tacgccgggc gggcgtggcg gatcgggttg
     2341 atggggcacg ccgccaccga gcgctccgtc gtgacgctgc tcggcgccac ccgggagctc
     2401 ctcaaggcct gatcgtctgt cgctgcggaa gcggaagccg tggcgccatt accctggaca
     2461 ggtggctaca tcgacggacc agcagctcaa ccgggtcacg ccggtcgagc tggtggacgc
     2521 cgttcgctcg ggacgcatcc gttacccgag tcctggcacc ccgtccgccg atgat
//
LOCUS       DASRSC010000294         9721 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_935309,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000294 DASRSC010000000
VERSION     DASRSC010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9721
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935309"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            53..244
                     /locus_tag="VFE39_14600"
     CDS             53..244
                     /locus_tag="VFE39_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259868.1"
                     /translation="MTGVAGPASPATRAAGGGALTQVAEWAGHSVGVLHQIYAKCIVG
                     QEDAARKRITAALAVKPDA"
     gene            complement(375..448)
                     /locus_tag="VFE39_14605"
     tRNA            complement(375..448)
                     /locus_tag="VFE39_14605"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(412..414),aa:Leu,seq:taa)
     gene            complement(460..1737)
                     /locus_tag="VFE39_14610"
     CDS             complement(460..1737)
                     /locus_tag="VFE39_14610"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6259869.1"
                     /translation="MNVIIIGGGIGGLCLAQGLRRAGIDVTVHERDRAPGSRWEGYRI
                     HINPVGARALHACLSDAGWQEFLATAGPGGDFGFLTEQLEQLVVVEESIMYPDGADPA
                     ESHYAADRATLRRLLRTGLEDVVQFGAEFVGYEQRPDGRVEATFADGRTAVGDVLVGA
                     DGAASRVRRQYLPHVRTVDAGAVSVAHKIWLTDEVRAALPRLGTGMNIVNVDAPCFLF
                     TSAFEPPGGASRPYLLCALVTRPDVLPPDLTELDGDALRAAVDVLVAAWHPQLRRALA
                     ESDPESRTAIAFRAAEPGPAWRSTPVTVIGDAIHLMPPIGGLGGNTALRDAHLLSRLL
                     AAVARGDQDTLAAIGEYESDMREYGTAAVRSALAQKEQALSHGAVATAGARTWFRLCA
                     AVPALRRRTFGQSYAGPAAPRPWERELAVTRDP"
     gene            complement(1734..2318)
                     /locus_tag="VFE39_14615"
     CDS             complement(1734..2318)
                     /locus_tag="VFE39_14615"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6259870.1"
                     /translation="MVGGARVDRREQLVAAAFRRVAEAGFEGLRLRQVAEDVGIDHST
                     LHHHFATKQDLIGAIAEYTTRQFWTTGPETSDPASALRGHLLALRRMVEDQPHLFAVT
                     VELDLRARRDPAVATAMARHEAGWRSFLTGVLVDGTTRGVWDLPVDAEATTELVIAAV
                     KGVRLAPEAAAQVFGQLEALLVGRAPDDTEGERL"
     gene            complement(2377..3789)
                     /locus_tag="VFE39_14620"
     CDS             complement(2377..3789)
                     /locus_tag="VFE39_14620"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6259871.1"
                     /translation="MARNTRIVVVGGGYVGMYTALRLQKKLRRGEADVTVIDPQSHMT
                     YQPFLPEASAGSIEPRHVVVPLRKVLRKCHHLTGRVTRITHATREVTVELVAGNVTTV
                     GYDVLVVAPGSVARTLPIPGLAECGIAFKTVGEAIYLRNHVLSRLDAAATTIDPTLRS
                     RLLTFLVVGGGYAGVEALAELQDMARYATRYYENITPEDLRWVLVEAAGRIMPEVGPK
                     MGRYTVERLLDAGIEVFLDTRVKTMEGGHVVLDDGTEFDTDTIVWTAGVKPNPMLEQT
                     DLPRDARGRVECIAELQVVDMPDVFSAGDCASVPDLSKDDPEAKTSPSAQHAVRQAKV
                     LADNIVAYLRGRQLTKYKHAYAGSVASLGLYKGVAEIYGIKLRGIVAWLMHRTYHVSR
                     MPTWNRRVRIVVDWTGALLLGREVVALGQINDPRAEFNRVANPTPPAQPAQRLAPQQQ
                     GAPVGAGQREEAVRPGLRGA"
     gene            complement(3819..4607)
                     /locus_tag="VFE39_14625"
     CDS             complement(3819..4607)
                     /locus_tag="VFE39_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET6259872.1"
                     /translation="MTRSSTGGAGFSGGLSAVTHPTLGDLDAALVECRACPRLVAWRE
                     LVAREKRAAFRDQEYWGRPVPGLGPEDASLLVVGLAPAAHGGNRTGRMFTGDRSGDVL
                     FAALHAVGLANQPTAVHADDGLELHHTRITSPVHCAPPDNKPTPAERDTCGGWLRAEL
                     ALLRPRAVVVLGAFGWQALLPVLAGAGWLVPRPRPRFGHGVHVELAGDRGPLHVFGCY
                     HVSQQNTFTGRLTSAMLEDVLRRAAHAAGIPPEWGLPPSRALDA"
     gene            4552..6003
                     /locus_tag="VFE39_14630"
     CDS             4552..6003
                     /locus_tag="VFE39_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HET6259873.1"
                     /translation="MTAESPPEKPAPPVDDLVTTSHTLRTPTGELAYTATTGRVVLRH
                     EKHSDGTFDGHEPQAEVFLVAYTLDGADPATRPVTVAFNGGPGSSSVWLHLGLLGPRR
                     VVMGDAGALTPPPWGLVDNPESLLAHSDLVFVDPVSTGYSRAVTGGKPADFHGYTPDV
                     ETIGEVVRLWITRNERWLSPKYLAGESYGTLRAAALAAYLQDRHGMYVNGLMLISSVL
                     DMGSIRFTEGNDRPYPLFLPTYAAIAHHHGFHPGRELREVLDDAEDFASGDYPWALAR
                     GSRLPTADLDRVAGRLAALSGLDEGYVRRVRLRIEHHRFFRELLRGTGRTVGRIDGRF
                     TGWEDDGGGEVTSYDPSIAAITGPYTAAFNSHVRSNLGYASDLPYEVLSGRVQPWSYR
                     DFEGRAVSVVGKLSAAMRANPHLRIHVACGYHDAATPYTAAEYVLAQLPIPDELRTGI
                     EIRYYEAGHMMYVHEPSRLAQSADLAAFVTGSR"
     gene            complement(6018..6971)
                     /locus_tag="VFE39_14635"
     CDS             complement(6018..6971)
                     /locus_tag="VFE39_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET6259874.1"
                     /translation="MPRVAAIDCGTNSIRLLVADVTTSADGTVSLRDVHREMRIVRLG
                     QGVDATGELAPEALERTRAALVDYAAVISREKAERVRMVATSATRDARNRDAFFGMVR
                     DTLGVDAEVISGDEEANLSFVGAVGDLDPDDGPFVVVDVGGGSTELVIGTLDGGKATV
                     QAARSVDIGSVRITERCLAGDPPTGEEIEAARELATAILDEAFDTVPVEGARTWVGVA
                     GTITTLSAVALGLAEYDADVVHRSRLTNEDLHRVTAELLGMSREERATHGAIHPGRID
                     VIGGGALVVDVLARELQARAGITELVVSEHDILDGIARSLV"
     gene            7403..9028
                     /locus_tag="VFE39_14640"
     CDS             7403..9028
                     /locus_tag="VFE39_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6259875.1"
                     /translation="MQPGDHRTHRVENQPPPLAGYDPLACDPALADAVRREGATEALP
                     ALGQLGAVMASPEAREHARLANEHEPVLRTHDRFGHRIDEVEFHPSWHWLLRTAVGWG
                     LHATPWVAEPGTGAHVARAAGFYLMSQLEAGHGCPISMTYAAVPALRHDPALAAAYEP
                     GLRSTTYQFGLAEPATKAGLLAGMSMTEKQGGSDVRAGTTSATPSADGTYQLVGHKWF
                     TSAPMNDLFLTLAQAPGGLTCFVLPRVLPDGTRNAIRLQRLKDKLGNRSNASAELEYA
                     GATGWRLGAEGRGVATIIEMVSMTRLDCVLGSAALTRAALTEAAHHVAHRSAFGSRLA
                     DQPLMQAVVADLALESEAATALALRLAGAVDRGERSLLRLALPASKYLVCKRAPTVVA
                     EALECLGGNGFVEESALPRLYREAPLNSIWEGSGNVTALDVLRAVTREPDSADAVLAE
                     VDAAAGADTWFDRGVRELRTELRGREERRARRLAEMLALCLQGSLLLRYAPDEVSSAF
                     VSSRFLPDGPWRTAGTLPAESHTAALLARVTPT"
     gene            complement(9039..9557)
                     /locus_tag="VFE39_14645"
     CDS             complement(9039..9557)
                     /locus_tag="VFE39_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HET6259876.1"
                     /translation="MSPATPRSPAPGISEADSAAVAAQLGRAPRAVREVAHRCPCGLP
                     SVVQTAPRLEDGTPFPTLYYLTCSRLNSRVGGLEADGRMREMTERLAADPELAAAYRR
                     AHDVYLAERDAIEPLGTDVTAGGMPDRVKCLHVCVAHALAAGPGVNPFGDEALAELGE
                     WWQPGPCARPSS"
BASE COUNT         1357 a         3581 c         3462 g         1321 t
ORIGIN      
        1 gagcggggct gggtagacgg tcacgccaca ccaggcacgg ggcggagaca gagtgactgg
       61 ggtggcgggg ccggcgtcgc cggccacgcg cgccgccgga ggcggcgcct tgactcaggt
      121 cgccgagtgg gccggacaca gcgtgggggt acttcaccag atctacgcca agtgcatcgt
      181 cggccaggag gacgcggccc ggaagcggat caccgcggcg ctcgcggtga agcctgatgc
      241 gtagaacttg ggcacgtatt gggcacagat acccgccaga cgccggtgtc cgccgcacac
      301 agccggacag cacaagatcg cctgccgacc gtgttgttgc tgctcagcgg gcaattttgc
      361 tggttgctgt ggggtgcccc cggtgggatt cgaacccaca ctggaaccct tttaaggggc
      421 ctgcctctgc cggttgggct acgggggcgc cggtccagcc tacggatcac gcgtcacggc
      481 cagctcgcgc tcccacggac ggggggcggc gggtccggcg tagctctggc cgaaggtgcg
      541 ccggcgcagt gcaggtaccg cggcgcacag ccggaaccag gtgcgtgcac cggccgtcgc
      601 cacggcgccg tgggacaggg cctgctcctt ctgcgccagc gcggagcgga cggctgcagt
      661 gccgtactcc cgcatgtcgg actcgtactc cccgatcgcc gccagggtgt cctggtcgcc
      721 tcgcgcgacc gcggccagca ggcggctgag caggtgcgca tcgcgcagcg cggtgttgcc
      781 gccgagcccc ccgatgggtg gcatgaggtg gatcgcgtcg ccgatgaccg tgaccggagt
      841 actccgccac gccggccccg gttccgcggc gcggaaggcg atcgcggtgc ggctctccgg
      901 atccgactcg gccagcgcgc gtcgcagttg cgggtgccag gcggcgacga gcacgtcgac
      961 cgcggcgcgc aacgcgtcgc cgtcgagctc ggtgaggtcg ggtgggagca cgtcggggcg
     1021 ggtcacgagg gcgcacagca ggtacgggcg ggacgccccg ccgggcggct cgaaggccga
     1081 ggtgaacagg aagcaggggg cgtcgacgtt cacgatgttc atgcccgtgc cgagccgcgg
     1141 cagcgcggca cgtacctcgt cggtgagcca gatcttgtgc gccacgctga ccgcgccggc
     1201 atcgactgtg cggacgtggg gcaggtactg ccggcggacg cgcgacgcgg cgccgtccgc
     1261 cccgacgagt acgtcgccca cggcggtgcg accgtctgcg aaggtcgctt ccaccctgcc
     1321 gtcgggacgc tgctcgtaac cgacgaactc ggcgccgaac tgcacgacgt cctccaggcc
     1381 ggtccgcagg agccgccgga gcgtggcgcg gtcggcggcg tagtggctct ccgccggatc
     1441 ggcgccatcg ggatacatga tcgactcttc gacgacgacg agctgttcca gctgttcggt
     1501 caggaacccg aagtcaccgc ccggccccgc ggtggcgagg aattcctgcc acccggcgtc
     1561 ggacaggcag gcgtgcagcg ctcgcgcgcc caccgggttg atgtggatcc ggtagccctc
     1621 ccatcggctg cccggggccc gatcccgctc atggacggtg acgtcgatgc ccgcccggcg
     1681 cagaccctgg gcgaggcaga ggccaccgat accgcccccg atgatgatca cattcacagt
     1741 cgctccccct ctgtgtcgtc cggcgcccga ccgaccagca gtgcctcgag ctgcccgaac
     1801 acctgcgccg cggcctcggg cgccagccgc acgcccttga ccgccgcgat gacgagctca
     1861 gtggtggcct cggcgtcaac cggcaggtcc cacacaccgc gagtggtgcc gtcgaccaac
     1921 actcccgtca gaaaggagcg ccaaccggcc tcgtgacgag ccatcgcggt ggccaccgcc
     1981 gggtcccgcc gggcccgcag gtccagctcg accgtgacgg cgaagaggtg gggctggtcc
     2041 tcgaccatcc ggcgcagcgc cagcaggtgc ccacgcaggg ccgaggcggg atccgaggtc
     2101 tccggtcccg tcgtccagaa ctgcctcgtc gtgtactcgg cgatggcacc gatgaggtcc
     2161 tgcttggtgg cgaagtggtg gtgcagcgtc gaatggtcga tgccgacgtc ctcggcgacc
     2221 tgacgcaggc gcagtccctc gaaccccgcc tcggccacac gccggaacgc cgcggcgacc
     2281 agttgctcac ggcgatcgac cctagctcca ccaaccactt ggttgaggct aggacaccac
     2341 ggggccgtct gccaccccac atgagaaacc gaacggtcag gcgccgcgga ggcccggtct
     2401 gacggcctcc tcgcgctggc ccgcgccgac cggagcaccc tgctgttgcg gtgcgagccg
     2461 ctgtgcgggc tgggccggcg gagtcgggtt tgcgacgcgg ttgaactcgg ccctcgggtc
     2521 gttgatctgg cccagtgcca ccacctcccg gcccagcaac agcgcgcccg tccagtcgac
     2581 gacgatgcgg acgcgccggt tccaggtggg catgcggctc acgtggtagg tgcggtgcat
     2641 gagccacgcg acgatcccgc gcagcttgat cccgtagatc tccgcgacgc ccttgtacag
     2701 cccgaggctc gccaccgacc cggcgtaggc gtgcttgtac ttggtgagct gccggccccg
     2761 caggtacgcc acgatgttgt cggcgagcac cttcgcctgc cgcaccgcgt gctgggccga
     2821 ggggctggtc ttggcctccg ggtcgtcctt cgacaggtcg ggcaccgagg cgcagtcacc
     2881 cgcgctgaac acgtcgggca tgtcgacgac ctgcagttcg gcgatgcact cgacgcggcc
     2941 cctggcgtcc cgcggcaggt cggtctgctc gagcatgggg ttgggcttga cgcccgcggt
     3001 ccagacgatc gtgtcggtat cgaactcggt gccgtcgtcg aggacgacgt ggccgccctc
     3061 catcgtcttc acgcgggtgt cgaggaagac ctcgataccg gcgtccagca gccgttcgac
     3121 ggtgtagcgg cccatcttcg gccccacctc gggcatgatc cgcccggcgg cctcgacgag
     3181 cacccagcgc aggtcctcgg gcgtgatgtt ctcgtagtac cgcgtggcgt agcgggccat
     3241 gtcctgcagc tcggcaaggg cctcgacacc ggcgtagccc ccgcccacca ccaggaacgt
     3301 gagtaaccgg ctgcgcagcg tcgggtcgat cgtggtggcc gcggcatcga gccgggacag
     3361 cacgtggttg cgcaggtaga tcgcctcacc gacggtcttg aacgcgatac cgcactcggc
     3421 caggccggga atcgggagcg tgcgcgcaac ggagcccggc gcgacgacca gcacgtcgta
     3481 gccgacggtg gtgacgttgc ccgcgacgag ctcgaccgtc acctcgcggg tcgcgtgggt
     3541 gatccgggtg acgcgaccgg tgaggtggtg gcacttcctc agcaccttcc gcagcggcac
     3601 cacgacgtgt ctcggctcga tggatccggc cgaggcctca ggaaggaacg gctggtaggt
     3661 catgtgcgac tgcgggtcga tcaccgtgac gtcggcctcc ccgcgccgca gcttcttctg
     3721 cagccgcagt gcggtgtaca tgccgacgta gccgccgcct accacgacga tcctcgtgtt
     3781 gcgcgccatg atgcaatggt cccactctca cggcgacttt acgcatcgag cgcacgtgac
     3841 ggtggcaacc cccattcggg tggtatcccc gcagcgtgtg cggcccggcg caggacgtcc
     3901 tccagcatcg ccgatgtgag tctgccggtg aaggtgttct gctggctgac gtggtagcag
     3961 ccgaacacgt gcagcggccc ccggtcgcct gccagctcga cgtggacccc gtgcccgaac
     4021 cggggccgag ggcgcggcac gagccaccct gctcccgcca gcaccggcag cagcgcctgc
     4081 cagccgaagg cgcccagcac caccaccgcg cgcgggcgca gcagcgccag ctcggcacgc
     4141 agccagccgc cgcaggtgtc acgctcggcc ggcgtcggct tgttgtcggg cggcgcgcag
     4201 tgcaccggcg aggtgatccg ggtgtggtgg agctcgagcc cgtcatccgc gtgcacggcc
     4261 gtcggctggt tggccagacc gacggcgtgc agggcagcga acagcacgtc gccggaccgg
     4321 tcgccggtga acatccgacc ggtccggttc ccgccgtggg cggcaggcgc gagcccgacg
     4381 accagcagcg aggcgtcctc cgggccgagc cccggcacgg gacgtcccca gtactcctgg
     4441 tcgcggaacg cggcgcgctt ctccctggcc acgagctcgc gccaggcgac cagccggggg
     4501 caggcccggc actcgacgag ggccgcgtcg agatcaccta gggtcgggtg tgtgactgcg
     4561 gagagcccac ccgagaagcc cgcaccgccc gtcgacgacc tcgtcaccac ctcacacacg
     4621 ctacgcacgc ccacgggtga gctcgcctac accgcgacca cgggcagggt ggtgctgcgc
     4681 cacgagaagc acagcgacgg caccttcgac gggcacgaac cgcaggccga ggtgttcctc
     4741 gtcgcctaca ccctcgacgg cgccgacccg gccacccgcc ccgtcacggt cgcgttcaac
     4801 ggcgggcccg gctcgtcgag cgtctggctg cacctcgggc tgctcgggcc acgacgcgtc
     4861 gtgatgggcg acgcgggcgc gctcaccccg cccccgtggg gtctcgtcga caacccggag
     4921 tcactgctcg cccactccga cctcgtattc gtcgatcccg tctccaccgg ctactcgcgc
     4981 gcggtcaccg gcggcaagcc cgccgacttc cacggctaca cgcccgacgt cgagacgatc
     5041 ggcgaggtgg tccggctgtg gatcacgcgc aacgagcggt ggctgtcgcc gaagtatctg
     5101 gccggtgagt cctacggcac cctgcgcgcc gccgcgctcg ccgcgtacct gcaggaccgg
     5161 cacggcatgt acgtcaacgg gctcatgctc atctccagcg tgctcgacat gggctcgatt
     5221 cgcttcaccg agggcaacga ccggccctac ccgctgttcc tgcccaccta cgcggcgata
     5281 gcgcaccacc acgggttcca tcccggtcgc gagctgcgcg aggtgctcga cgacgccgag
     5341 gacttcgcct ccggcgacta cccctgggcg ctcgcccgcg gctcccggct ccccactgcc
     5401 gacctcgacc gggtggccgg gcggctggcc gccctcagcg ggctcgacga gggctacgtg
     5461 cgccgggtgc gcctgcggat cgagcaccac cggttcttcc gggagctgct gcgcggcacc
     5521 ggtcggaccg tcggccggat cgacggccgg ttcaccggct gggaggacga cggcggcggc
     5581 gaggtgacca gctacgaccc gtccatcgcg gcgatcaccg ggccctacac cgcggcgttc
     5641 aactcacacg tgcggagcaa cctgggctac gccagtgacc tgccctacga ggtgctgagc
     5701 gggcgggtgc agccctggtc gtaccgggac ttcgagggcc gcgcggtgtc ggttgtcggc
     5761 aagctctcgg cggcgatgcg cgccaacccg cacctgcgca tccacgtggc ctgcggatac
     5821 cacgacgcgg ccacgccgta cacggcggca gagtacgtgc tcgcccagct gccgatcccc
     5881 gacgagcttc gtaccgggat cgagatccgc tactacgaag ccgggcacat gatgtacgtg
     5941 cacgagccct cgcggctggc gcagtcggcc gacctggcgg cgttcgtgac ggggagccgg
     6001 tagctcggcg gccgggctca gacgagtgag cgcgcgatcc cgtccaggat gtcgtgttcg
     6061 ctcaccacga gctcggtgat gccggcgcgg gcctgcagct cgcgcgccag cacgtcgacg
     6121 accagggcac ccccgccgat cacgtcgatc cggccgggat ggatggcgcc gtgggtggcg
     6181 cgctcctccc gagacatgcc gagcagctcg gccgtcaccc ggtgcagatc ctcgttcgtg
     6241 agccgggacc ggtgcacgac gtcggcgtcg tactcggcca gacccagcgc cacggccgac
     6301 aaggtggtga tcgtaccggc gacgcccacc caggtgcgcg cgccctccac cggcaccgtg
     6361 tcgaacgcct cgtcgaggat cgcggtggcc agctcgcggg cggcctcgat ctcctccccg
     6421 gtcggcggat cgcccgccag gcagcgctcg gtgatccgga cgctcccgat gtccaccgag
     6481 cgggcggcct gcaccgtcgc cttgccgccg tccagtgtcc cgatgacgag ttcggtggag
     6541 ccgccgccca cgtcgaccac aacgaatggc ccgtcgtcgg gatcgaggtc gccgaccgcc
     6601 ccgacgaacg acaggttcgc ctcctcgtcg ccggagatca cctcggcgtc gacgccgagc
     6661 gtgtcgcgga ccatcccgaa gaacgcgtcg cggttgcgcg cgtcacgggt ggcgctcgtg
     6721 gcgaccatcc gcacccgttc tgccttctcc cggctgatca ccgcggcgta gtcgacgagt
     6781 gcggcgcgcg tgcgctccag tgcctccgga gccagctcgc cggtcgcgtc gacgccctgt
     6841 ccgagccgga cgatgcgcat ctcgcggtgc acgtcacgca gggagacggt gccgtcggca
     6901 ctggtcgtga catcggcgac gagcagccgg atcgagttcg tcccgcagtc gattgcggcc
     6961 acgcgcggca tgcgctctcc tcggtctggt ccaggggtcg cgcccacggt acgcggggcg
     7021 ccgcactcga acagggcgac ccgaaccgcg agtagcacac gaacgagccg tgcgccgcta
     7081 ccctcggccc gtggtcgacc cccaaaggca cgagaagggc cgggcgggca ccggcgcatc
     7141 gacgtcagct ccggccggct ccgctccgac ccctcgcggc acggccggcg ggctgtccat
     7201 cccggcccaa ccccggcggc cgagccgggc gaggcgcacg gcgggcgttg ccgaaccggc
     7261 gacgtccgga cagccgacaa cggacggccg accgccaggg gcagcaaccg ttgccggggc
     7321 gaccggagtg gaccagccga gcacgggcgc gccgccttcc accctctccg cctcccgggt
     7381 cgagcaaccg gctgaaccgc ccctgcagcc cggcgaccac cgcacgcacc gcgtcgagaa
     7441 ccagccgcca cccctggccg gctacgaccc gctggcctgc gatcccgcgc tcgccgatgc
     7501 cgtccggcgg gagggcgcga cagaagccct gccggctctg ggccagctcg gtgctgtcat
     7561 ggcgtcgccg gaggcgcggg agcacgcacg gctggcgaac gagcacgagc ccgtccttcg
     7621 tacgcacgac cggttcggtc accgcatcga cgaggtcgag ttccacccgt cgtggcactg
     7681 gttgctccgc accgcggtcg ggtggggact gcacgcgacc ccatgggtgg ccgagcccgg
     7741 aaccggcgcc cacgtggcgc gggccgcggg cttctacctg atgagccagc tcgaggccgg
     7801 acacggctgc ccgatctcga tgacgtacgc cgctgttcct gcgctgcgcc acgaccccgc
     7861 gctcgcggcg gcctacgagc cgggcctgcg gtcgacgacc taccagttcg ggctggccga
     7921 gccggcaacg aaagccggac tgctggcggg catgtcgatg acagagaagc agggcggctc
     7981 cgacgtccgc gccggcacca catcggccac cccgtcggcg gacgggacgt accagctcgt
     8041 cggtcataaa tggttcacct cggcgccgat gaacgacctg ttcctcacac tcgcccaggc
     8101 ccccggcgga ctcacctgct tcgtgctgcc ccgcgtgctg ccggacggaa cgcgcaacgc
     8161 catccggttg caacggctca aggacaagct gggcaaccgc tctaacgcgt cggccgagct
     8221 cgagtacgcc ggcgccaccg gctggcggct cggcgccgag ggacgcggcg tcgcgacgat
     8281 catcgagatg gtgtcgatga cccgcctcga ctgcgtgctg ggctccgccg ccctcacccg
     8341 ggcagccctg accgaggcgg cgcaccacgt ggcgcaccgg agcgcgttcg ggtcccggct
     8401 ggccgaccag ccgttgatgc aggccgtggt cgccgacctg gcgctggagt ccgaggcggc
     8461 caccgccctg gccctgcggc tcgccggcgc cgtcgaccgg ggagaacgct cgctgctccg
     8521 gctcgcgctc ccggcgtcga agtacctcgt gtgcaagcgg gcgccgaccg tggtggccga
     8581 ggcgctcgag tgcctcggcg gcaacggctt cgtcgaggag tcggcgctgc cgcggctcta
     8641 ccgcgaggcg ccgctgaact cgatctggga gggctcgggc aacgtcaccg cgctcgacgt
     8701 cctgcgcgcg gtgacccgcg agccggactc cgcggacgcg gtgctggcgg aggtggacgc
     8761 cgccgcgggg gcggacacct ggttcgaccg cggcgtccgc gagctgcgca cggagctgcg
     8821 gggccgcgag gagcggcggg cccgccggct cgccgagatg ctcgccctgt gcctgcaggg
     8881 gtcactgctg ctgcggtacg cacccgacga ggtctcgtcg gcgttcgtga gctcgcgctt
     8941 cctccccgac ggcccgtggc gcacggccgg caccctgccg gcggagtcgc acacagcggc
     9001 tctgctggcg cgggtcaccc ccacctgacg ggcgcggatc aggacgaagg ccgggcgcag
     9061 gggcccggct gccaccactc ccccagctcg gcgagtgcct cgtcgccgaa ggggttcacg
     9121 ccgggacctg cggcgagggc gtgcgcgacg cacacgtgga ggcatttcac ccggtccggc
     9181 ataccgcctg cggtgacgtc ggtgcccagc ggctcgatcg cgtcgcgctc ggcgaggtag
     9241 acgtcgtggg cccgccggta ggccgcggcg agctccgggt ccgcggccag ccgctcggtc
     9301 atctcgcgca tccggccgtc ggcctcgagg ccgccgacac gcgagttcag ccgggagcag
     9361 gtcaggtagt acagcgtcgg gaacggggtg ccgtcctcca gccgcggggc agtctgcacc
     9421 accgacggga ggccgcacgg gcagcggtgt gccacctcgc ggacggcccg cggcgcgcgt
     9481 ccgagctgcg ctgccacggc ggcggagtcg gcctcgctga tgcccggggc aggcgagcgc
     9541 ggcgtcgccg ggctcattgc gggcccgcgg cctcgcgcca cagctgccgg taccacggca
     9601 cgcccgcggc gctcgctccc gcaccggtgg cggacgggtc gaccggcagt tgcacgacgt
     9661 agggcacctc ccccggcgtg acgtagccga gccgggacct ggcctgggcg gcgacctcgg
     9721 c
//
LOCUS       DASRSC010000295         9599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3718475,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000295 DASRSC010000000
VERSION     DASRSC010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3718475"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="VFE39_14650"
     CDS             complement(<1..1505)
                     /locus_tag="VFE39_14650"
                     /inference="COORDINATES: protein motif:HMM:NF039636.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6049 family protein"
                     /protein_id="HET6259877.1"
                     /translation="MKPVAALVAIPVLVLTALTLAGLVLGGPFPTPASAAPARAAAAP
                     FQPEPDEIAGPLRLQLAQLGPRMVTADGPRDLTITGTLTNTGDQPIGQLQIRVQRGIP
                     LGTEGEVRDALDGSAATDATTPRFTSLPGELAPGSQMPVQLTVPLRGAPPTSLALDST
                     GVYELLVNVNGVPRDGQRARLAAVRMLLPVLSLPPDPDEPTAVPSATTGGATPFALLY
                     PIADTPRRVATVPGEPTLLTDDDLAASMAPDGRLGGLVAALAQNAPAGSRVRAATCLA
                     IDPDLVETASLMRAGYEVLGAQGAAPVPGTGAEVAGRWLDSLAAVAKGGCVIALPYAD
                     ADLVALTRGGLGALASSTIRDGRQVLADILHTPVVPGVTWPVDGVTDDPTLDQIAAAD
                     GRSLVLSANGVEHGGVQRHSGVVPIAGGRQPQFAVLTDPLLSRAASGPTSFTEPDPGS
                     GGAVASATPAGAVGPLSTQDAIGALAFRTKEGPGRGAGAAGPLVLAPPHQWA"
     gene            complement(1502..1996)
                     /locus_tag="VFE39_14655"
     CDS             complement(1502..1996)
                     /locus_tag="VFE39_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HET6259878.1"
                     /translation="MSTSRGRSGGHRAGRPSDRRRRLRTVDETSAGGLVVDHASGTAA
                     VIGRLDRRGRLLWSLPKGHIEAGETAEEAAVREVEEETGIIGRVVAPLGTIDFWFVAE
                     DRRVHKTVHHFLLRALGGELSDSDVEVSEVAWVPLDELESRLAYADERRLIRRATELM
                     EETA"
     gene            2098..3564
                     /locus_tag="VFE39_14660"
     CDS             2098..3564
                     /locus_tag="VFE39_14660"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET6259879.1"
                     /translation="MSATALAPGAVPPDLVRSQENAVVEMVQIGPVAEELAARFTQAG
                     HRLYLVGGSVRDALLGRESGDLDFTTDARPDAVLAVLAGWADAIWDTGIAFGTVGARR
                     HGSTVEITTFRADAYDRVTRNPVVAFGDTIEDDLVRRDFTVNAMAVELTVDERRFVDP
                     HGGLAALAAGTLDTPATPEESFADDPLRMLRAARFVSQLGLAPAPRVVAAMTAMASEL
                     GRITRERVQVELTKLVCGAHPRRGIELLVETGLAEQVLPEVPAMQLAVDEHMQHKDVY
                     THSLVVMEQAIEREKPGSPDLVLRLAALLHDIGKPDTRRREPDGRVSFHHHEVVGAKM
                     VRRRLRELRYPKAIIDDVAQLVFLHLRFHGYGNGEWTDSAVRRYVTDAGPLLDRLHKL
                     VRSDCTTRNRRRAAALQRSYDSLEDRIAQLRQQEELDAIRPDLDGNAIMQLLGIPPGP
                     LVGKAYKHLLAVRMERGPLSHEEAEAELRSWASENRVG"
     gene            complement(3574..4914)
                     /locus_tag="VFE39_14665"
     CDS             complement(3574..4914)
                     /locus_tag="VFE39_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259880.1"
                     /translation="MSTSGPPTPPLAVGTPTATATERLGFARLVRTRGYRRLLAVRFA
                     TQWADGVFQAALGGAVLFNPERQADPLAVAAGLAVLLLPYSVIGPFAGALLDRWDRKR
                     VLMSANLARAALVVVVAAIVFAGVTGPGLYLAALAVAGISRFVNSGLSVALPHVVPRR
                     HLVEANTLAVTAGAAMAAIGGASAIGIRQLLGPDDTGSAITTLAAVAGSVLAATLAAG
                     FGRSALGPDRTDPGSRAAVTVLHGLADGARATAATPSVAASFLALAAHRLSFGITTLL
                     TLLLFRYAFTDSGPFRAGLAGIGEAVLLAAAGLGTAALLAPWMVRRWGRPRTIRVALL
                     VATVTQLALAALLSMPAVLVAAFVLGATGQIVKLCTDAAVQGEAGDGVLGRVFALYDI
                     VFNVGYVLAVAVAALLSPSDGNAPWLLGAAALLYVLGLLAHDQQLRRRPRTLAG"
     gene            4956..5555
                     /locus_tag="VFE39_14670"
     CDS             4956..5555
                     /locus_tag="VFE39_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HET6259881.1"
                     /translation="MVDDRSGSGASPGSEPVPGSLLVAAPGLLDPNFRRTVIFMIEHR
                     ERGSLGVVLNRPSEVTVRDVLPSWASLSAQPRAVFVGGPVEAETALCLATVRTGHDPA
                     VIEGLVRVRGPVVLVDLDTDPTMIGPSLRGLRVFAGYSGWDAGQLAGEIDRGDWIVVP
                     ALPDDVLSRQDGGLWGHVLRRQGMPLSLLATYPADVRQN"
     gene            complement(5604..6062)
                     /locus_tag="VFE39_14675"
     CDS             complement(5604..6062)
                     /locus_tag="VFE39_14675"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HET6259882.1"
                     /translation="MPLPLRLILGSLLVLAGVALVAVAVFGARSRLRRNAWVGVRTPA
                     TLLSETAFAVANRAAAPPAGAAGAVAIAGGAVLLAGTSGTLGLVVLAVTVVGVLVLAG
                     VAGAVGDRAAAAVAQPVAGPPACGGSCAGCDLVAGCRDASTSVPNGSDHG"
     gene            6251..9151
                     /gene="leuS"
                     /locus_tag="VFE39_14680"
     CDS             6251..9151
                     /gene="leuS"
                     /locus_tag="VFE39_14680"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET6259883.1"
                     /translation="MSTDTTAAAPRRADGGADDAALPYRYGAPLAAGIERRWQDRWEA
                     EGTFHAPNPSGPWADPAGLGGDKVYLLDMFPYPSGSGLHVGHPLGFIGTDVLGRYLRM
                     TGHTVLHAMGFDAFGLPAEQYAVQTGTHPRTTTEDNIRRYKAQLRQLGLGHDERRSVA
                     TTDVEFYRWTQWIFLQIYNSWYDEAQQRARPIAELEAEYAAGTRPTPDGRSWGELTSV
                     EQRGVVDSHRLAYISEAPVNWCPGLGTVLANEEVTADGRSERGNFPVFRRSLKQWMMR
                     ITAYADRLVADLDRLDWPDSVKAMQRNWIGRSEGAQVRFPVAGVATPIEVFTTRPDTL
                     FGATYMVLAPEHPLVDAITPDAWPSDVDARWTGGAATPSEAVAAYRDAAARKSDLDRQ
                     ENREKTGVFIGAYATNPVNGQPIPVFVADYVLMGYGTGAIMAVPAQDQRDWDFATAFG
                     LPIVRTVQPTEGWDGEAYTGDGPAINSANDEISLDGLGIGEAKRAITDWLAARGAGEP
                     VVQYKLRDWLFSRQRYWGEPFPIVWDADGMPVALPDDQLPVALPEIDDYSPKTYAPDA
                     ADTEPEPPLSRASEWVEVDLDLGSGPRHYRRETNTMPQWAGSCWYYLRYLDPENEERF
                     VDPEVERYWLGKDPARGPADPGGVDLYVGGVEHAVLHLLYARFWHKVLYDLGQVSSEE
                     PFRRLFNQGYIQAFAYTDARGAYVPAEEVAESTAPDGTVGFTWNGQPVRREYGKMGKS
                     LRNVVTPDEMCERYGADTFRLYEMSTGPMDVSRPWSTRDVVGSQRFLQRVWRNLVDER
                     TGEARVTDAEPEVETLRALHRAIAGVHTDFAALHYNTAAAKLIELNNHLTKTYGSTGV
                     PRSVAEPLVLMMAPLTPHVAEELWALLGHEDSLAHGPFPVADEQYLVAETVEYPIQVN
                     GKVRSRIVVPADAGTATVEAAALAEEKVAGLLDGREPRKVIVVPGRLVNVVV"
     gene            complement(9185..>9599)
                     /locus_tag="VFE39_14685"
     CDS             complement(9185..>9599)
                     /locus_tag="VFE39_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6259884.1"
                     /translation="VSTWRRQFEVNVVAVAELTRLLLPALRAARGRVVLLNSGAGLSA
                     SPGWASYAASKFALTAFADALRAEEAVNGLRVTSVHPGRVDTEMQRGVVAQEGGEYHA
                     ETYLRPESVAGAVLLAVTAPDDAALTELVLRPAAR"
BASE COUNT         1407 a         3426 c         3530 g         1236 t
ORIGIN      
        1 gcccactggt gcggcggggc gagcacgagc gggccggccg ccccggcgcc ccggccagga
       61 ccctccttgg tgcggaaggc gagcgcgccg atggcgtcct gggtggacag cggaccgacg
      121 gcgcccgcgg gagtggcgga ggccaccgcc ccgccgctgc cggggtccgg ttcggtgaac
      181 gaggtgggcc cgctggcggc gcggctgagc aggggatcgg tcagcaccgc gaactgcggc
      241 tgccgtccgc cggcgatggg caccacaccg ctgtggcgct gcaccccgcc gtgctcgacg
      301 ccgttcgcgg acagcaccag tgaacgaccg tcggccgcgg cgatctggtc cagcgtgggg
      361 tcgtcggtca cgccgtcgac gggccaggtg acaccgggta cgaccggggt atgcaggatg
      421 tcggcgagca cctgtcttcc gtcgcggatc gtcgagctcg cgagcgctcc caacccacct
      481 ctcgtcagag cgacgagatc cgcgtccgcg tagggcagag cgatcacgca gccgcccttg
      541 gccaccgcgg cgaggctgtc caaccatcgg ccggccacct cggcccccgt cccgggcacc
      601 ggggcggctc cttgtgcccc gagaacctcg taacccgcgc gcatcaggga tgccgtctcc
      661 acgaggtcgg gatcgatcgc gagacaggtc gcagcccgca ccctggaccc ggccggagcg
      721 ttctgggcga gcgccgcgac caggccgccg agccgcccgt ccggagccat ggacgcggcg
      781 aggtcgtcgt cggtgagaag ggtgggttcg cccggcacgg tcgcgacccg ccgcggcgtg
      841 tcggcgatcg ggtacagcag cgcgaacgga gtggcgccgc cggtggtggc ggagggcacc
      901 gccgtcggct cgtcggggtc gggtggcagc gacagcacgg gcagcaacat gcggaccgcg
      961 gccaaccgcg cccgctggcc gtcgcggggc accccgttga cgttgacgag cagctcgtac
     1021 acgccggtgg agtcgagcgc gaggctggtc gggggtgcgc cccgcagcgg gaccgtcagc
     1081 tggaccggca tctggctgcc cggtgcgagc tccccgggaa gcgacgtgaa ccggggagtc
     1141 gtggcgtcgg tggccgcgct gccgtccagc gcgtcgcgca cctcgccctc cgtgcccagc
     1201 ggaatgccgc gctgcacgcg gatctgcagt tgcccgatcg gctggtcgcc ggtgttcgtc
     1261 agcgtgccgg tgatggtgag gtcgcgaggg ccgtcggccg tgaccatgcg gggaccaagt
     1321 tgggccagtt gcagccggag tggtccggcg atctcgtcgg gctccggctg gaacggggcg
     1381 gcggcggccc gagccggggc cgcggacgcc ggggtcggga acggcccacc gaggaccagc
     1441 cccgccagcg tcagcgccgt gagcaccaga actgggatcg cgaccagggc cgcgaccggc
     1501 ttcacgccgt ctcctccatg agctccgtgg cccgcctgat caagcgacgc tcgtcggcgt
     1561 aggccagccg cgactcgagc tcgtcgagcg gcacccacgc cacctccgag acctcgacgt
     1621 ccgagtcgga cagctcgccg ccgagtgcgc gcagcaggaa gtggtgcacg gtcttgtgca
     1681 cgcgccggtc ctcggcgacg aaccagaagt cgatggtgcc cagtggtgcg accacgcggc
     1741 cgatgatgcc ggtctcctcc tcgacctcgc gcaccgccgc ctcctcggcc gtctcaccgg
     1801 cctcgatgtg gcccttgggc agggaccaca gcaggcggcc ccgccggtcc agcctgccga
     1861 tcaccgcggc cgtgcccgat gcgtggtcca cgaccagccc accggccgac gtctcgtcga
     1921 ccgtccgcag ccgccgacgg cggtccgagg ggcgacccgc acggtgcccc ccggagcggc
     1981 cgcgggatgt ggacatgcgg cgatcgtatc gagcagacgg gttacgttgc cgtcacagcg
     2041 ctgccggccc ggcatcctcg tcgctgcgtc ccagcggggc gcctacactg gcccgccgtg
     2101 tccgcaacag ccctggcgcc cggcgccgtc ccccccgacc tcgtacgttc gcaggagaat
     2161 gcggtcgtgg agatggtgca gatcgggccg gtcgccgagg agttggcggc ccgcttcaca
     2221 caggccgggc accggctcta cctcgtgggc ggaagcgtcc gggacgccct gctcgggcgc
     2281 gagtccggag acctcgactt caccacggat gcgcggcccg atgcggtcct cgcggttctg
     2341 gcgggctggg ccgacgcgat ctgggacacc ggcatcgcct tcggcacggt cggcgcgcgc
     2401 aggcacggca gcaccgtcga gatcacgacg ttccgggccg acgcgtacga ccgcgtcacg
     2461 cgcaatccgg tcgtggcctt cggcgacacc atcgaggacg acctcgtccg gagggacttc
     2521 accgtcaacg cgatggcggt ggagctcacg gtcgacgagc ggcgcttcgt cgatccccac
     2581 ggcgggctcg ccgcactcgc cgccggcacc ctcgacacgc cggcgacacc cgaggagtcc
     2641 ttcgccgacg acccgctacg gatgctgcgg gccgcgcggt tcgtctccca gctgggtctg
     2701 gcacccgcgc cgcgagtcgt cgcggcgatg actgcaatgg cctccgagct gggccgaatc
     2761 acccgcgagc gcgtgcaggt cgagctgacc aagctggtct gcggcgcgca cccgcgccga
     2821 ggaatcgagc tgttggtcga gacagggctc gccgagcagg tgctgccgga ggtgccggcg
     2881 atgcaactgg ccgtcgacga gcacatgcag cacaaggacg tctacacgca ctccctggtc
     2941 gtcatggagc aggcgatcga acgcgagaag cccggatctc cggatctcgt gctgcgcctc
     3001 gccgcgctgc tgcacgacat cggcaagccc gacacgcggc gcagggagcc cgatgggcgc
     3061 gtcagcttcc atcaccacga ggtggtcggg gccaagatgg tgcggcgacg gctgcgggag
     3121 ctgcgctacc ccaaggcgat catcgacgac gtcgcccagc tggtgttcct gcacctgcgc
     3181 tttcacggtt acggcaatgg cgagtggacc gactccgcag tgcgccggta cgtcaccgac
     3241 gccggacccc tgctggaccg gctgcacaag ctcgtgcgct ccgactgcac cacgcgcaac
     3301 cggcgccgcg cggctgctct gcagcgcagc tacgactcgc tcgaggaccg gatcgcgcag
     3361 ctgcgtcagc aggaggagct cgacgccatc cgtccggacc tggacggcaa cgcgatcatg
     3421 cagctcctcg gcatcccacc gggcccgctc gtcgggaagg cgtacaagca cctgctcgcc
     3481 gtgcgcatgg agcgcggtcc gctgtcacat gaggaggcgg aggccgagct acggagctgg
     3541 gcgtccgaga accgtgtggg ctgagctcgg acgtcaaccc gccaaggtgc gggggcggcg
     3601 gcgtagctgc tggtcgtgcg ccagcaggcc gagcacgtag aggagggccg ctgctccgag
     3661 gagccatgga gcgttgccgt cggaggggct gagcagggcg gcgacagcca ccgcgagcac
     3721 gtagccgacg ttgaagacga tgtcgtagag cgcgaacacc cgtccgagca ctccgtcgcc
     3781 cgcctccccc tgtacggcgg cgtcggtgca gagtttgacg atctggcccg tggcgccgag
     3841 cacgaacgcc gcgaccagca ccgccggcat cgacaggagc gcggcgagcg cgagctgcgt
     3901 gaccgtggcg acgagcagcg ccacgcggat ggtgcgggga cgaccccacc gccggaccat
     3961 ccacggtgcg agcagcgccg ccgtcccgag gcctgccgcg gcgagcagga cagcctcgcc
     4021 gatccccgcg aggcctgcgc ggaacggccc gctgtccgtg aaggcgtacc ggaacagcag
     4081 cagcgtgagc agcgtcgtga tcccgaacga gagccggtgg gcggcgagcg ccaggaagga
     4141 tgcggccacc gaaggcgtgg cggcggtggc gcgcgcgccg tcggcgagtc cgtgcaggac
     4201 ggtgacggcg gcacgtgagc ccgggtccgt ccggtccggt ccgagggcgc tccggccgaa
     4261 gccggcggcc agggtggcgg cgagcaccga tccggccacg gccgccagcg tggtgatcgc
     4321 ggagccggtg tcgtccgggc cgaggagctg gcggatcccg atcgcgctgg ccccgccgat
     4381 cgccgccatg gccgcccctg ccgtgacggc gagagtgttg gcctcgacga ggtgacgacg
     4441 gggaacgacg tgcggaagcg cgaccgacag cccggagttg acgaaccggc tgatcccggc
     4501 cacggcgagg gcggcaaggt acagaccggg tccggtgacc ccggcgaaga cgatggcggc
     4561 gacgaccacg acgagcgcgg ctcgcgccag gttggcactc atcagcacgc gcttgcggtc
     4621 ccaccggtcc agcagcgccc ccgcgaacgg gccgatcacc gagtacggca acagcagcac
     4681 ggccagcccc gccgcgacgg cgagcgggtc ggcctgccgc tccgggttga acagcacagc
     4741 cccgccgagc gcagcctgga acacgccgtc ggcccactgc gtggcaaagc gcacggcaag
     4801 cagcctgcgg tagccgcgag tacgaaccag ccgggcgaaa cccagtcgct cggtcgccgt
     4861 cgccgtgggc gttcccacgg cgaggggcgg cgtcggtggt cccgatgtcg acacggccga
     4921 aggttacgcg gccgggcgga tgcaacgatg gtcccgtggt ggacgaccgc tccggctctg
     4981 gcgcttctcc cggttccgag ccggtaccgg gcagcctgct cgtggcggcg cccggacttc
     5041 tcgatcccaa cttccggcgc accgtcatct tcatgatcga gcaccgggag cggggcagcc
     5101 tcggcgtggt gctgaatcgg ccgagcgagg tgacggtgcg tgacgtcctg ccgtcctggg
     5161 catcgctgtc cgcgcagccg cgcgcggtct tcgtcggcgg cccggtggag gccgagacgg
     5221 cactgtgtct cgccacggtg cggaccggcc acgatccggc cgtgatcgag gggttggtgc
     5281 gcgtgcgcgg ccccgtggtg ctggtcgacc tcgacaccga cccgaccatg atcggaccca
     5341 gtctccgcgg cctgcgggtg ttcgccggct actccggctg ggacgccggg cagctcgccg
     5401 gggagatcga tcggggcgac tggatcgtgg tgcccgcgct gcccgacgac gtgctgtccc
     5461 ggcaggacgg cggtctgtgg ggccacgtgc tgcgccggca gggcatgccg ctgtcgctgc
     5521 tggcgaccta cccggcggac gtccggcaga actgacgccg gtccgcgcgg cgcgactcca
     5581 cctgcgtggg tgaggcccgg cggtcagccg tggtcggagc cgttcgggac gctggtgctc
     5641 gcgtcgcggc agccggccac gaggtcgcag cccgcgcacg agcccccgca ggcgggtggt
     5701 ccggcgaccg gctgtgccac tgccgccgcg gctcggtccc cgaccgcgcc ggcaacgccg
     5761 gcgagcacga gcaccccgac gacggtcacg gcgagcacga ccaggccgag cgtgccgctc
     5821 gtgccggcga gcaggacagc cccgcccgcg atggcgacgg cgcctgccgc gcctgccggc
     5881 ggcgccgccg cccggttggc tacggcgaac gccgtctccg agagcaaggt cgccggcgta
     5941 cggaccccga cccaggcgtt gcgacgcagc cgcgagcgcg ctccgaacac ggcgacggcg
     6001 acgagcgcga cgccggccag cacgagcagc gagccgagga tcagacggag tggcagcggc
     6061 acggcgacca gggtaggcga acccgatcgc gggcccaccg ccagcggagt tacgctgggt
     6121 acgtggccca ccgactcctc gtcgaggccg cgccgcacta gcagaaccgg gcgcggccga
     6181 cccctcatgc ccgagcggca ggggggtctt ctggttttcg ggccgactag agacttccca
     6241 ggagtgggac atgagcacgg acacgaccgc agctgcacct cggcgcgccg acggcggtgc
     6301 cgacgatgcg gcgctgccct accggtacgg cgcgccgctg gccgcgggga tcgaacgccg
     6361 ctggcaggat cggtgggagg ccgagggcac gttccacgcc cccaacccgt cgggtccgtg
     6421 ggccgacccc gccggcctcg gcggcgacaa ggtctacctg ctggacatgt tcccgtaccc
     6481 ctccggatcc gggttgcacg tcggccaccc gctggggttc atcggcacgg acgtgctggg
     6541 gcgctacctg cggatgaccg gccacaccgt gctgcacgcg atgggtttcg acgcgttcgg
     6601 tctgcccgca gagcagtacg cggtgcagac gggcacgcac ccgcgcacga ccacagagga
     6661 caacatccgg cgctacaagg ctcagctgcg ccagctgggc ctgggccacg acgagcggcg
     6721 ctcggtggcc accaccgacg tcgagttcta ccgctggacg cagtggatct tcctgcagat
     6781 ctacaacagc tggtacgacg aggcgcagca gagggcgcgg cccatcgccg agctggaggc
     6841 ggagtacgcc gcaggcaccc gtcccacccc cgacgggcgg agctggggcg agctgacgag
     6901 cgtcgagcag cgcggggtcg tcgactcaca ccggttggcg tatatctccg aggcccccgt
     6961 caactggtgc cccgggctgg gcacggtgct ggccaacgag gaggtcaccg ccgacggccg
     7021 cagcgagcgt ggcaacttcc ccgtgttccg gcgcagcctg aagcagtgga tgatgcggat
     7081 caccgcttac gccgaccggc tggttgccga cctggaccgg ctggactggc ccgactcggt
     7141 caaggcgatg cagcgcaact ggatcggccg ctcggagggc gcgcaggtgc gctttcccgt
     7201 cgccggcgtc gcgacgccga tcgaggtctt caccacccgc cccgatacgc tgttcggcgc
     7261 cacctacatg gtgctggcgc ccgagcaccc gctggtcgac gcgatcaccc ccgacgcgtg
     7321 gccctccgac gtcgacgcgc ggtggacagg tggggccgcc accccgagcg aggccgtcgc
     7381 cgcgtaccgg gacgccgcgg cccgcaagtc ggatctggac cggcaggaga accgggagaa
     7441 gaccggtgtc ttcatcggcg cctatgccac caacccggtc aacggacagc cgatccccgt
     7501 cttcgtcgcc gactacgtgc tgatgggcta cggcaccggc gcgatcatgg ccgtgcctgc
     7561 tcaggaccag cgcgactggg acttcgccac ggcgttcggc ctgccgatcg tccgcaccgt
     7621 gcagccgacc gagggctggg acggcgaggc ctacacgggt gacggtccgg cgatcaactc
     7681 ggcgaacgac gagatcagcc tggacgggct gggcatcggc gaggccaagc gcgcgatcac
     7741 cgactggctg gccgcgcgcg gggccggcga gccggtcgtc cagtacaagc tgcgcgactg
     7801 gctgttctcc cggcagcggt actggggcga gccgttcccg atcgtgtggg acgccgacgg
     7861 catgccggtc gcgctgccgg atgaccagct gcccgtcgcg ctgccggaga ttgacgacta
     7921 ctcacccaag acctacgccc ccgacgccgc ggacaccgag ccggagccgc cgctgtcacg
     7981 cgcgtcggag tgggtggagg tcgacctgga cctggggtcg gggccacggc actaccgccg
     8041 ggagaccaac acgatgccgc agtgggccgg ctcctgttgg tactacctgc gctatctgga
     8101 cccggagaac gaggagcgct tcgtcgatcc ggaggtcgag cggtactggc tgggcaaaga
     8161 tccggcccgt ggccccgccg atccgggcgg cgtcgacctg tacgtcggcg gtgtagagca
     8221 cgccgtgctg catctgctgt acgcccgctt ctggcacaag gttctgtacg accttggcca
     8281 ggtcagctcc gaggagccat tccgccggct gttcaaccag ggctacatcc aggccttcgc
     8341 ctacaccgac gcccgtggtg cgtacgtgcc tgcggaggag gttgccgaat cgacagcccc
     8401 ggacggcacg gtcggtttca cgtggaacgg gcagcccgtc cgccgggagt acgggaagat
     8461 ggggaagtcg ctgcgcaacg tggtgacgcc cgacgagatg tgcgagcgct acggcgccga
     8521 caccttccgt ctctatgaga tgtcgacggg cccgatggac gtctcccgtc cgtggtccac
     8581 ccgtgacgtg gtcggctcgc agcgcttcct gcagcgtgtg tggcgcaacc tggtggacga
     8641 gcgcacgggc gaggcccgcg tcaccgacgc ggaaccggag gtcgagacgc tgcgtgccct
     8701 gcaccgcgcg atcgctgggg tgcacaccga cttcgcggcg ctgcactaca acaccgccgc
     8761 ggccaagctg atcgagctga acaaccacct gacgaagacc tacggcagca ccggtgtgcc
     8821 gcgcagcgtc gccgagccgc tggtgctgat gatggcgccg ctgaccccgc acgtcgccga
     8881 ggagctgtgg gcgctgcttg gccacgagga ctcgctggcc cacggtccgt ttccggtggc
     8941 cgacgagcag tacctggtgg ccgagacggt cgagtacccg atccaggtca atggcaaggt
     9001 ccgctcccga atcgtcgtcc ccgccgatgc cggcaccgcg acggtggagg cagcggcgct
     9061 ggccgaggag aaggtcgccg gactgctgga cggccgggag ccgcgcaagg tgatcgtcgt
     9121 acccggccgc ctggtcaacg tggtggtcta gccggcccgc ggtgccacgg gaggtcgtcc
     9181 ccggtcagcg ggcggcgggt cgcagcacga gctcggtgag ggctgcgtcg tcgggcgctg
     9241 tgacggccag cagcacggcc ccggccaccg actcgggccg caggtaggtc tcggcgtggt
     9301 actcgccgcc ctcctgcgcg acgacacccc gctgcatctc ggtgtcgacg cgaccggggt
     9361 gcaccgaggt gacccgtagg ccgttgacag cctcttcggc ccgcagtgcg tcggcgaacg
     9421 ccgtcagcgc gaacttgctc gcggcatagg acgcccagcc aggagaagcg gagagtccgg
     9481 ctcccgagtt cagcaggacg acgcgcccgc gggcggcccg cagcgccggt agcagcagcc
     9541 tggtcagctc ggccacggcc acgacgttca cctcgaactg gcgacgccac gtcgagacc
//
LOCUS       DASRSC010000296         5551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4052070,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000296 DASRSC010000000
VERSION     DASRSC010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4052070"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VFE39_14690"
     CDS             <1..397
                     /locus_tag="VFE39_14690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259885.1"
                     /translation="NGTIFGSRGRGRGLLAATLVAAALALGGCGAAGTARLAPAVTAD
                     GVQRVEVAFAGGVVTGGLRRYAVPLGSTVELVVASDVADEVHVHGYDRLSYVTAGATT
                     SVRFVADLPGVFDVELEQRGLPLTQLEIA"
     gene            590..1495
                     /locus_tag="VFE39_14695"
     CDS             590..1495
                     /locus_tag="VFE39_14695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259886.1"
                     /translation="MAVERIMRFLLLVALTALLLPGGATPALAHAGGLTSSSSEARVL
                     AIDPPVPGLDVRAVEFGARLRVDNGTGDTVVVEPLPGSQLSGLPVVAPGGRAYWTDPR
                     VTAAAAGERPDGDRVAWAVPLRVGATAVTVRGEQFWPPGPATAQWVGVALLALAVPAV
                     AGVLGAARRWGRVVLAVATVAVIGAHLVHVLGSAGVPEDQPYPLMLLSAAGYALLGWP
                     LGAVGAWLTVRGHAAGPLLCVAAGGLFAIVITPVDAFTLVDAVVPYAWGADLDRALVA
                     LTLGGGLGVVVAGMAVLRRGTVPAS"
     gene            1496..2716
                     /locus_tag="VFE39_14700"
     CDS             1496..2716
                     /locus_tag="VFE39_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259887.1"
                     /translation="MVLAHGIGSVEGLPLNGELVLQTGGVVVLVSFLAVALLWRRPRF
                     AGPVTGRPLPAVLDAPSLRSALQAVTLAGAVAIVGYGFAGPQDPDANPAPRALYVLLW
                     VGLVPASLVFGPVWRVLNPLRLLHRLTATALRRPVHGTAPLSDRLGYLPAAAGLLVFV
                     WLELVAPGRDRPAVVATFLLAYAVLHTLAALWFGERWFDRADAFEAYSALTGALAPIG
                     RLDTGRIGLRNPFRGLAAVPVAPGLVGFVAVWWGSTVFDGLTGWVGWQALGLPPVLDT
                     LVLLGLVLMVAGLYRAATGALAGPLVTTLVPIAAGYTIAHYVTLLLVEGPRGLTQLAG
                     AQLGAVTAVPAPAIVAGAQIAAVLVGHVVAVFAAHDRSVALLPEHRRLADQVPLVLLM
                     VGYTMAGLFLLVIS"
     gene            2721..3050
                     /locus_tag="VFE39_14705"
     CDS             2721..3050
                     /locus_tag="VFE39_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HET6259888.1"
                     /translation="MMVARSVLLFVLAAVAEIGGAWLVWQGVREQRGLLWIGAGVLAL
                     GAYGFVATLQPDPHFGRILAAYGGIFVAGSLAWGVLVDGFRPDRLDYIGAALCLVGVA
                     VIMYVPR"
     gene            complement(3058..3981)
                     /locus_tag="VFE39_14710"
     CDS             complement(3058..3981)
                     /locus_tag="VFE39_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019812960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HET6259889.1"
                     /translation="MGAGHGHGTEATAGGSASGRYVRRLAISLGVLVAFLVLEVVVGL
                     ATSSLALLSDAGHMFTDVLGVGMALAAIAAARASRRTDRRTFGLYRAEVLAALANAVL
                     LFGVAAWVLVEAVQRFANPPDVPGLPLMLTAAAGLAANLVVFAMLRKGAQESLNVRGA
                     YLEVLADTLGSVGVLLAGAVTLLFGWRYADPIVAIGIGLFVLPRTWSLARQAVRILVQ
                     AAPAHVDMAAIRSELVSLEGVQDVHDVHVWTLTSGMEVGSAHLTVDADCDPAVVLTDA
                     QRLLADRHHLAHFTLQVEPPGARTGCTELAW"
     gene            4182..5225
                     /locus_tag="VFE39_14715"
     CDS             4182..5225
                     /locus_tag="VFE39_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020501957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET6259890.1"
                     /translation="MADRADLPRVAVFSLGGTIASTSTPDERGAAGGVTPRLGAGQLV
                     EAVPQLRDVAELETVSFRQIPSGDLTLRDLVDLAAEITRRFDRGVSGVVVTQGTDTIE
                     ETSFALDLLVRGPRPVVVTGAMRNPTLPGSDGPANLLAAVQVAVAPAHAGSGAIVVMN
                     DEIHAARFVRKTHTSSPAAFRSSTAGPWGWIVEGRPRFAFRLPALGTRFSSVCSDRIP
                     AVALLTSTLGDDARLIAQVGKLGYGGLVLEAFGGGHVPAHVVPALQDLGERVPVVLAS
                     RTGGGETLQKTYGSPGAERDLLGRGLIAAGFLDGLKARILLSLALAAGADIPAVREAF
                     RQVNMSAVDGSCT"
BASE COUNT          662 a         1973 c         2049 g          867 t
ORIGIN      
        1 gaacgggacc atcttcggca gccgaggccg aggccgcggc ctgctcgccg cgacgctggt
       61 ggcggctgcg ctcgccctcg gtggatgcgg ggcggcaggt accgccaggc tcgcgcccgc
      121 cgtcacggcg gacggcgtgc agcgcgtgga ggtggcgttc gcaggcggcg tcgtcaccgg
      181 cgggttgcgg cggtacgcgg tgcccctcgg gtccaccgtc gagctcgtgg tggccagcga
      241 cgtggccgac gaggtgcacg tgcacggcta cgaccggctg agctacgtca ccgcgggcgc
      301 caccacctcc gtgcggttcg tcgccgacct gccgggggtc ttcgacgtgg agctggagca
      361 gcgcggcctc cccctgacgc aactggaaat cgcctgaccc cgccccaccc cgcgagtcgc
      421 ccgattcccg cccgcgagtc gcccggttcg tgcgcgagtc gcccgattcc cgcccgcgag
      481 tcgccccgaa cgactcgcca ggatgcgcga gtcgcccggg agccgggttc ccgtgcgccg
      541 ggtcatggcg agtcgcgggc ggggtggcgg aggctcgtag ggtgagaacg tggcagtgga
      601 gcggatcatg cggttcctcc tgctcgtcgc tttgacggcc ctcttgctgc ccggcggtgc
      661 aacgccggcc ctcgcgcacg ccggcgggct gacgtcgtcc agcagcgagg cgcgggtgct
      721 cgcgatcgac cccccggtac cagggctgga cgtgcgcgcc gtggagttcg gcgccaggct
      781 ccgggtggac aacggcaccg gcgacacggt cgtcgtcgag ccgctgcccg gttcccagct
      841 ctcaggccta ccggtcgtcg caccgggtgg ccgggcctac tggaccgacc cccgcgtcac
      901 ggccgccgct gccggagagc gcccggacgg tgatcgggtg gcatgggctg ttccgctgcg
      961 ggtcggagcc accgcggtga cggtgcgcgg cgagcagttc tggccaccgg gaccggccac
     1021 cgcgcagtgg gtgggcgtcg ccctgctcgc actggcggtc ccggccgtgg ccggtgtgct
     1081 cggggcggca cgccggtggg gccgggtcgt gctcgccgtc gcgacggtcg ccgtcatcgg
     1141 tgcgcacctg gtgcacgtcc tcggctccgc cggggtgccc gaggaccagc cgtacccgtt
     1201 gatgctgctc agcgccgcgg ggtacgcgct gctcggttgg ccgctcggcg cagtcggcgc
     1261 gtggctgacg gtgcgtgggc atgccgcggg tcccctgctg tgcgtcgctg cggggggcct
     1321 gttcgccatc gtcatcaccc cggtcgacgc cttcactctc gtcgacgccg ttgtgccgta
     1381 cgcctggggt gcggatctcg accgcgcgct cgtggcgctg accctcggcg gcgggctcgg
     1441 cgtcgtcgtc gcggggatgg cggtgctgcg gcgcgggacg gtcccggcgt cgtagatggt
     1501 gctcgcgcac ggcatcggaa gtgtcgaagg gctgccgttg aacggtgagc tcgtgctgca
     1561 gaccggcggc gtcgtcgtgc tcgtgtcctt cctcgcggtg gcgctgctgt ggaggcggcc
     1621 gcgcttcgcc ggtccggtga ccggacgacc cctgcccgcc gtcctggacg ccccgtcact
     1681 gcggtccgcg ctgcaggcgg tgactctggc gggggcggtt gcgatcgtcg ggtacggctt
     1741 cgccggcccg caggacccgg acgccaaccc cgctccccgc gcgctctacg tgctgctgtg
     1801 ggtggggctc gttccggcgt cgctggtgtt cgggccggtg tggcgggtgc tgaacccgct
     1861 ccggctgctg caccgcctca ccgcgacggc cctacgccgc ccggtgcacg ggacggcgcc
     1921 cctgtccgat cgactcggct accttccggc tgcggccggg ctgctggtgt tcgtctggct
     1981 ggagctggtg gcgcccggac gggaccggcc cgccgtcgtc gcgacgttcc tgctggccta
     2041 cgccgtcctg cacacgctgg cggcgctgtg gttcggcgag cgctggttcg accgcgctga
     2101 cgccttcgag gcctactccg cactcaccgg agcactcgca ccgatcggtc ggctggacac
     2161 cgggcgcatc gggctgcgca acccgttccg ggggctcgcg gccgtgcccg ttgcgccggg
     2221 gctcgtcggt ttcgtcgcgg tgtggtgggg ctccaccgtg ttcgacggcc tcaccggctg
     2281 ggtgggctgg caggcgctgg gcctgccgcc ggttctcgac acgctcgtgc tcctcgggct
     2341 ggtactgatg gtcgccgggc tctaccgggc cgcaacaggg gcgctggcgg gtccgctggt
     2401 gaccaccctg gtcccgatcg cggctgggta cacgatcgcc cactacgtca cgttgctgct
     2461 ggtggagggg ccgcgtgggc tcacgcagct cgccggcgcc cagctcggcg ccgtcaccgc
     2521 tgtgcccgca cctgcgatcg tggccggcgc ccagatcgcc gccgtgctcg tcggacacgt
     2581 cgtggccgtc ttcgccgcgc acgaccgcag cgtggctctg ctgcccgagc accggcggct
     2641 cgccgatcag gtgccgttgg tgctgctgat ggtgggctac acgatggccg ggttgttcct
     2701 gctcgtgatc tcctgacgcg atgatggtgg cgcgatcggt cctcctgttc gtgctcgcag
     2761 ccgtcgccga gatcggcgga gcatggttgg tgtggcaggg cgtgcgtgag caacgcgggc
     2821 tgctgtggat cggggcggga gtactggcgc tcggggccta cgggttcgtc gccacgctgc
     2881 agccagaccc gcatttcggc cgcatccttg ccgcgtacgg cgggatcttc gtggcgggat
     2941 cgctggcgtg gggggtgctg gtggacggtt tccgcccgga ccgcctcgac tacatcggcg
     3001 cggcgctgtg cctcgtcggc gtcgccgtga tcatgtatgt gccacgctga tgccgcatca
     3061 ccatgcgagt tccgtgcagc ccgtacgggc ccccggcggc tcgacctgca aggtgaagtg
     3121 ggccaggtgg tggcggtcgg cgaggaggcg ctgcgcatcg gtgagcacga cggccgggtc
     3181 gcagtccgcg tcgaccgtca ggtgcgcgga accgacctcc atcccggagg tcagcgtcca
     3241 gacgtgcacg tcgtgcacgt cctgcacgcc ctccagcgag acgagctcgg atcggatggc
     3301 tgccatgtcc acgtgcgcgg gcgcggcctg tacgaggatc cggaccgcct gacgggccag
     3361 cgaccaggtg cgcggcagta cgaagagccc gatcccgatg gcgacgatcg ggtccgcgta
     3421 gcgccagccg aacagcagtg tcaccgcacc ggcgagcaac accccgaccg aacctagcgt
     3481 gtcggcgagc acctcgaggt aggcgccacg cacgttgagg ctctcctgcg cgcccttgcg
     3541 cagcatcgcg aacacgacga ggttcgcggc gagcccggct gcggcggtga gcatcagcgg
     3601 caggcccggc acgtccggcg ggttggcgaa gcgttgcacg gcctccacga gtacccaggc
     3661 ggccacgccg aacagcagta cggcgttggc gagcgcggcg agcacctcgg cgcggtagag
     3721 cccgaacgtc ctgcggtcgg tacgtcggga tgcgcgggcc gccgcgatgg cggcgagagc
     3781 cataccgacg ccgagcacgt cggtgaacat gtgcccggcg tccgagagca gcgccagcga
     3841 cgacgtcgcc aacccgacga cgacctcgag tacgaggaac gcgacgagca caccgagcga
     3901 gatcgcgagg cgccgcacgt aacgtcccga cgcactacca ccggccgtcg cttccgtgcc
     3961 gtgaccgtga cccgccccca tggcccacaa catatgcgga agcgtgcatg agtgcaaacg
     4021 ggcgggggca agccgcgcag cgcccgcccc ggccgcctcg tcgcagccat ccgcgatgct
     4081 tgtgaccgtg acctcgcccg gatccccctc cgtccgaaag ccggcaccgg ctgtgatcgc
     4141 ttgccacgct gtccccgctg tcgcgccggc cggaggaaac ggtggccgat agagccgacc
     4201 tgcctcgcgt agcagtcttc tccctgggcg gcacgatcgc ttcgacgagc acgccggacg
     4261 agcgcggggc agcggggggt gttactccgc ggctcggggc aggtcagctc gtcgaggctg
     4321 tgccccagct tcgggatgtc gccgaactgg agacggtgtc cttccgtcag atcccctccg
     4381 gcgacctgac gctgcgagac ctcgtcgatc tggccgccga gatcacccgg cgcttcgacc
     4441 ggggggtgag cggcgtcgta gtcacgcagg ggacggacac gatcgaggag acgtcctttg
     4501 cgctcgacct gctcgtgcgc ggtccgcggc cggtggttgt caccggcgcg atgcgcaatc
     4561 ccaccctccc ggggtcggac ggacctgcca acctcctcgc agccgtgcag gtggcggtcg
     4621 ctccggccca cgcgggctcg ggagcgatcg tcgtcatgaa cgatgagatc cacgccgcgc
     4681 gcttcgtgcg caagacccac acctcgagcc cggccgcgtt ccggtcgagc acagcgggcc
     4741 cgtggggctg gatcgtcgaa ggacgacctc gtttcgcgtt ccgcctgccc gcgttgggga
     4801 cgcgcttctc ctcggtgtgc tcggaccgga tcccagccgt cgccctgctc acgtccacac
     4861 tgggagacga tgcgcgactc atcgcccagg tggggaagct cgggtacggc gggctggttc
     4921 tcgaggcctt cggcggcggc cacgtccctg cccacgtcgt accggcgttg caggacctcg
     4981 gcgagcgtgt cccggttgtg ctggcctcgc gcacaggcgg cggcgagacg ctccagaaga
     5041 cctacgggtc tccgggcgcg gagcgggatc tgcttggccg cggactcatc gcggctgggt
     5101 tcctcgacgg cttgaaggcc cggatcctgc tgagcctggc cctggccgcc ggcgccgata
     5161 tacctgccgt ccgcgaggcg ttccggcagg tcaacatgtc cgctgtcgac ggcagctgca
     5221 cgtagcagaa gggggcgggc ggaagtgata ggtccatggg gcctcacgca cccgggccgg
     5281 gtcgatgtca tcggcacccg cgcctcgtcc cgggctgagc gagagacccg gagcgcgttc
     5341 ggttgctcag gaccccacga gggcggccac ccggaccatc tcgttgccga cgatcatcat
     5401 gccctcacct cccgtgcccg gcggccagga tctgatccgc tccgcatgcc acggccacgt
     5461 gcgccatcac ctgtgctgaa cggtccgtct cgttgcaggc accgccgcag taggcggcga
     5521 ggccgcggcg ccgcacggac accgatccgt a
//
LOCUS       DASRSC010000297         7983 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_610990,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000297 DASRSC010000000
VERSION     DASRSC010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_610990"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..176
                     /locus_tag="VFE39_14720"
                     /pseudo
     CDS             111..176
                     /locus_tag="VFE39_14720"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(314..1390)
                     /locus_tag="VFE39_14725"
     CDS             complement(314..1390)
                     /locus_tag="VFE39_14725"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HET6259891.1"
                     /translation="MALDESGPSKERPGASAAEPRLVAGRYALGRVLGIGSSAVVHRA
                     RDLWGGADVAVKRFHSGASAHDLRQQRQEMEVLARLDHPGLVGLHGGGTEDGRSFVVT
                     DLVEGPTLAERIRTGPPLAPGAVRTLGRHLAEALAHVHSGGIIHRDVKPANVLLDGVR
                     PRLADFGIARALEGAGATASGCVVGTAAYLAPEQVRGERVGPAADVYALGLVLLEALT
                     GRREYPGLAMESATARLHRAPAVQAGLPGGLGDLLEAMTRDDPERRPSAAQVAALLAA
                     DPAYRVRRSPAGVSRGRHRLPGRGRRKPATARFVLTMFTGAVAVLAGAAPPAASDAVI
                     SVSDQNAAVPAAPTVIPPGGLLGE"
     gene            complement(1454..2266)
                     /locus_tag="VFE39_14730"
     CDS             complement(1454..2266)
                     /locus_tag="VFE39_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HET6259892.1"
                     /translation="MIPVAFSYARPDSLESALALLEEHGEDATVLAGGHSLLPVMKLR
                     LAAPEVVIDIGRLSELHYIHLDGDEVAIGAGTRHRDLEASDVLGAEVPLLAAVARTVG
                     DPQVRHRGTIGGSLAHADPASDLPAAVLTLGGTVVLRGPRGERQVPATGFYTGMFSSV
                     KEPDELIVEIRVPRTGSAGWAYEKFTRRANDWAIVGVAVVDGRVGLVNMGATPLRASA
                     TEAALADGASIEEAAALAAQGTDPTSDIPATAEYRRHLARVLTRRALTTAAG"
     gene            complement(2263..2823)
                     /locus_tag="VFE39_14735"
     CDS             complement(2263..2823)
                     /locus_tag="VFE39_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HET6259893.1"
                     /translation="MKVGITVNGTETTADVEPRLLLAHYLRDVIGLKATNVGCDTTSC
                     GACTVLVDGESVKSCTVLAVQADGQSVTTMEGLSPNSTKLHPVAAAFRAEHGLQCGFC
                     TPGMVMATVGLLKENPKPTEREVREGLEGNLCRCTGYHNIVRAVLAASGQDPDAGQTT
                     TVEADADRSPGGGVESAPGFSTGSGA"
     gene            complement(2844..5138)
                     /locus_tag="VFE39_14740"
     CDS             complement(2844..5138)
                     /locus_tag="VFE39_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HET6259894.1"
                     /translation="MSIMGTRVVRTEDPVFLSRGATYTDDLTDERLNGALHLTLVRSP
                     LAHARITAVDVEAAREAPGVVAVFAGADIDLAPTLLFAGANPGMVRPWLATDKVRFVG
                     EPVAAVLTEQSYQGQDAADLVEIDYDPLPAVVDPRAALTDEVLLFEEVGTNLAGGFGH
                     DEEFDEHFFDDCEVVVTRDIVNQRLAASPLEGRAAAAVWGDDGRVTLWASTQNPQSAR
                     DEVASWLGIDAGLIHLIAPDVGGGFGAKIGADPEFALVVWLAQRLKRAVRWTETRSEN
                     LTGMLQGRAQLQTITIGGNRDGDVLAYRLDVLADAGAYPRLGVFLPFFTRQMAPGVYD
                     IPKVESRARTVVTTTTSTGAYRGAGRPEATAAIERAMDLFAAEIGKDPAEVRRRNVVP
                     PDKFPFETKGGAVYDSGEYAKAIDRVLEGAGYAELRAEQARRRERGDVVQLGIGVSAY
                     VEITGGGAFSEDASVEVHADGSVTVLTGTSPHGQGHATAWAMLTSDRLGIPIEKITVK
                     HGDTDLIPRGAGTMGSRSLQTGGVAVHQAAGELVELAKQRAADLLEASVDDLEVADGA
                     VSVRGTDSNVTLAQLADREQLKVDTVFDSGAPTFPFGAHVAVVEVDIESGKAVVDRIV
                     TLDDAGPIVNPLLAEGQRHGGIAQGISQALLEEVVYDADGNPLTATFADYAFPSAAEL
                     PSFTLLDMATPTTLNPLGAKGIGEAGTIGATPAVQSAVIDAVSHLGVRHIDMPTSPLR
                     VWEAINAARGERGAGSDSSEGAQQ"
     gene            complement(5307..6959)
                     /gene="thiC"
                     /locus_tag="VFE39_14745"
     CDS             complement(5307..6959)
                     /gene="thiC"
                     /locus_tag="VFE39_14745"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HET6259895.1"
                     /translation="MTALEDRTVAPQVTTGPIRGSHKAYLDGPDDLRVPVRRIELGTG
                     EHHDVYDTSGQYTDDSVTIEVRDGLPALRAPWIAAREPVGGAATQLAWARAGVITPEM
                     RFIAIRESVDVELVRSEVARGRAVIPANRCHPEAEPMIIGKAFLVKINANLGNSAVTS
                     SIEEEVEKMVWATRWGADTVMDLSTGKNIHETREWILRNSPVPIGTVPIYQALEKVGG
                     DPTALSWEVYRDTVIEQCEQGVDYMTVHAGVLLRYVPLTAKRVTGIVSRGGSIMAAWC
                     LAHHRESFLYTRFEELCDILRTYDVTFSLGDGLRPGSIADANDEAQLAELRTLGELTA
                     VARARDVQVMIEGPGHVPLHKIAENVRLEEEWCGEAPFYTLGPLATDIAPGYDHITSA
                     IGAATIAARGTAMLCYVTPKEHLGLPDRDDVKTGVITYKIAAHAADLAKEHPRAQARD
                     DALSAARFEFRWLDQFHLSLDPDTALSFHDETLPAEPAKSAHFCSMCGPKFCSMRITQ
                     DVRDYAEAHGLTSVEAIEAGMSEKSDEFTDAGGRVYLPLSDA"
     regulatory      complement(6951..7065)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(7098..7955)
                     /gene="thiD"
                     /locus_tag="VFE39_14750"
     CDS             complement(7098..7955)
                     /gene="thiD"
                     /locus_tag="VFE39_14750"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET6259896.1"
                     /translation="MSERAVGVSPPRVMTIAGTDSGGGAGIPADLRAFAACGVHGCVA
                     VTAVTVQNSVGVTGVHPIPPETVAAQIEAVASDIGLDAAKTGMLASRPIIEAIAGACD
                     RVGIGGTGSTPLVVDPVAASMHGDQLLADDALDAFRALLFPRATVITPNLDEVRLLVG
                     IDVHDRSAQYEAAKVLHGLGPQYVLVKGGHLHEDTDGCVDLLYDGHTFTELPGPRFDT
                     GNTHGGGDSMASAIASGLARGMAVPDAVALGKRYIVEAVRHSYPLGAGHGPVSPLWAV
                     RPWWEEPTP"
BASE COUNT         1116 a         2890 c         2811 g         1166 t
ORIGIN      
        1 gctcgccccg gagatcaccc ggcgggccat cgcgcgcctc accccgccca cgcgccgccc
       61 ggaaccgccg cccggtatcg accgacgacc tcaccccccg cgagaccgac gtgctcacct
      121 gcctggggcg cggcctgtcc aacgccgaga tcgccgcgga gctggtgatc acagaagcga
      181 ccacgaagac gcacgtatcc cgggtgctca ccaagctcgg gctacgttcc cgtgcaggcg
      241 gcgatcgcgg cgcaggatgt gggccagcct ggtcggttga gtccggggtg ccgacgcggc
      301 ccccgggtcc gggctactcg cccagcaaac caccgggcgg aatgacggtg ggcgctgcgg
      361 gaaccgcggc gttctggtcc gagacggaga tgacggcgtc ggaggccgcg ggcggtgcgg
      421 ccccggcgag caccgcgaca gccccggtga acatagtcag cacgaaccgg gccgtcgcgg
      481 gcttccgccg accgcggccg ggcagccggt ggcgcccgcg cgacacacct gccggcgacc
      541 gccgcacgcg gtacgccgga tccgcggcga gaagtgccgc gacctgggcg gcggagggcc
      601 ggcgttccgg gtcgtcccgc gtcatggctt cgaggagatc ccccaacccg ccgggcagcc
      661 cggcctgcac cgccggagcg cggtgcaacc tcgcggtcgc cgactccatc gcgaggcctg
      721 ggtactcgcg gcgtccggtc agggcctcca gcagcacgag ccccagcgca tagacgtccg
      781 cggccgggcc cacgcgctcc ccgcgcacct gctccggcgc gaggtaggcg gcggtgccga
      841 ccacgcaccc gctcgcggtt gccccggctc cctcgagcgc gcgggcgata ccgaagtccg
      901 cgagccgcgg ccggacaccg tcgagcagaa cgttggcggg cttcacgtca cggtggatga
      961 tcccgccgct gtgcacgtgg gcgagcgcct cagccaggtg cctgccgagc gtccgcaccg
     1021 caccgggtgc cagcggtggg cccgttctga tccgctcggc cagcgtcggt ccctcgacga
     1081 ggtcggtcac gacgaacgac ctgccgtcct ccgtgccgcc gccgtgcagg ccgacgagac
     1141 cggggtggtc gagtcgcgcc agcacctcca tctcctgccg ctgctggcgc aggtcgtgcg
     1201 ccgatgcacc ggaatggaag cgcttcaccg ccacgtccgc gccgccccat aggtcgcggg
     1261 cacggtgcac caccgctgac gagccgatgc cgagcacccg gccgagcgcg taccggccgg
     1321 cgacgagccg tggctcggcc gcagacgcgc caggccgttc cttcgacgga ccggactcgt
     1381 cgagggccat gcggaccggt tcccccgatg ggtccgatcc atgcgtaatc cgaaggatcg
     1441 gccccgaacg agatcagccg gcggcggtgg tgagcgcccg gcgggtgagg acccgggcca
     1501 ggtgcctgcg gtactcggca gtggccggga tgtcgctcgt ggggtcggtg ccctgggcgg
     1561 ccagcgcagc ggcctcctcg atcgatgcgc cgtccgccag tgcggcctcc gtggccgagg
     1621 ctcgcagggg tgtcgcgccc atgttgacca gcccgacgcg accgtcgacc acggccactc
     1681 cgacgatcgc ccagtcgttg gcgcgacggg tgaacttctc gtaggcccac ccggccgagc
     1741 cggtgcgcgg caccctgatc tccacgatca gctcgtccgg ctccttcacc gaggagaaca
     1801 tcccggtgta gaagccggtg gcgggcacct ggcgctcccc gcgcggaccg cgcagcacga
     1861 cggtgccgcc gagcgtgagc actgcggcgg gcaggtcgga cgccgggtcg gcgtgggcga
     1921 gggacccgcc gatggtgccg cggtggcgga cctgcgggtc gccgaccgtg cgggcgaccg
     1981 ccgcgagcag cggtacctcg gcgccgagca cgtcggaggc ctccaggtcg cggtgccgcg
     2041 ttccggcgcc gatcgcgacc tcgtcaccgt cgaggtggat gtagtgcagc tcggacagcc
     2101 gtccgatgtc gatgaccact tcgggcgcag cgagccgcag cttcatcacg ggcagcagcg
     2161 agtggccgcc tgccagcacc gtggcgtcct ccccgtgctc ctcgagtagc gccagcgcgc
     2221 tctcgaggga gtccgggcgg gcgtaggaaa aggcgaccgg gatcacgcgc cgctcccggt
     2281 ggagaagccc ggcgcggact cgacgccacc gcccggggac cggtcggcat ccgcctcgac
     2341 ggtggtcgtc tgcccggcgt cgggatcctg tccgctggcg gccagcacgg cgcggacgat
     2401 gttgtggtaa cccgtgcacc ggcagaggtt gccctcgaga ccctcacgca cctcacgctc
     2461 ggtgggcttc gggttctcct tcagcaggcc gaccgtggcc atcaccattc cgggggtgca
     2521 gaagccgcac tgcagtccgt gctcggcgcg gaacgcagca gccaccgggt gcagcttcgt
     2581 ggagttcggg gacagcccct ccatcgtggt gaccgactgc ccgtcggcct gcaccgcgag
     2641 tacggtgcac gacttcacgg actcgccgtc caccagcacc gtgcaggcgc cgcacgaggt
     2701 ggtgtcgcat ccgacgttcg tggccttgag gccgatcacg tcacgcagat agtgcgcgag
     2761 cagcaggcgc ggctcgacgt cggccgtggt ctccgtgccg ttgacggtga ttccgacctt
     2821 cacggtcgtg ttcattgagg tggtcactgc tgcgctccct cggagctgtc gctgccggct
     2881 ccgcgttcgc cacgggcggc gttgatggcc tcccacaccc gcagcgggct ggtgggcatg
     2941 tcgatgtggc ggactccgag gtgggagacg gcatcgatca ccgcgctctg caccgcagga
     3001 gtcgcaccga tggtgcccgc ctcgccgatg cccttggccc cgagcgggtt gagggtggtc
     3061 ggcgtggcca tgtccagcaa cgtgaagctg ggcagctcgg ccgcggacgg gaaggcgtag
     3121 tcggcgaagg tggccgtcag cgggttgcca tcggcgtcgt agacgacctc ctcgagcagc
     3181 gcctgggaga tgccctgggc gatgccgccg tggcgctggc cctccgcgag cagcgggttc
     3241 acgatcggtc cggcatcgtc gagcgtgacg atccggtcga ccaccgcctt gcccgactcg
     3301 atgtcgacct ccacgaccgc gacgtgcgcg ccgaacggga acgtcggcgc cccgctgtcg
     3361 aacacggtgt cgaccttcag ctgctcccga tccgcgagct gggcgagcgt gacattcgag
     3421 tccgtacccc tgaccgacac cgcgccgtcg gcgacctcca ggtcgtcgac gctcgcctcc
     3481 agcaggtcag ccgcacgctg cttcgccagc tccacgagct cgcccgccgc ctggtggacg
     3541 gcgacgccgc cggtctgcag gctgcgcgag cccatggtgc ccgcgccccg cgggatgagg
     3601 tccgtgtcgc cgtgcttcac cgtgatcttc tcgatcggga tgcccaggcg gtcgctcgtg
     3661 agcatcgccc atgcggtggc gtggccctgg ccgtgcggtg aggtgcccgt caggaccgtg
     3721 accgacccgt cggcgtgcac ctcgacggag gcgtcctcgc tgaacgcgcc gccaccggtg
     3781 atctcgacgt acgcggagac cccgatgccg agctggacga cgtcgccacg ctcgcggcga
     3841 cgcgcctgct cggcgcgcag ctcggcgtag cccgcaccct ccagcacacg gtcgatcgcc
     3901 ttggcgtact cgccgctgtc gtagacggcg ccgcccttgg tctcgaaggg aaacttgtcc
     3961 ggcggcacca cgttgcgcct gcggacttcg gccgggtcct tgccgatctc ggcggcaaac
     4021 aggtccatgg cccgctcgat cgccgcggtg gcctccggac gcccggcgcc gcggtaggcg
     4081 cccgtggacg tggtggtggt gacgacggtg cgggcccgcg actcgacctt ggggatgtca
     4141 tagacccccg gcgccatctg gcgggtgaag aacgggagga acacgccgag gcgcggatag
     4201 gcgcccgcgt cggccagcac gtcgaggcgg taggcgagca cgtcgccgtc acggttgccg
     4261 ccgatcgtga tggtctgcag ctgcgcgcga ccctgcagca tcccggtgag gttctccgag
     4321 cgggtctcgg tccagcgcac cgcacgcttc aggcgctgcg cgagccacac cacgagcgcg
     4381 aactcggggt cggccccgat cttcgcgccg aagcccccgc cgacgtccgg ggcgatcagg
     4441 tggatcagcc cggcgtcgat acccagccac gaggcgacct cgtcccgggc cgactgcggg
     4501 ttctgggtgg aggcccacag ggtgacgcgc ccgtcgtccc cccagaccgc cgcggccgcc
     4561 cggccctcca ggggagaggc ggcgaggcgc tggttgacga tgtcgcgcgt gacgaccacc
     4621 tcgcagtcgt cgaagaagtg ctcgtcgaac tcctcgtcgt ggccgaagcc gccggcgagg
     4681 ttggtgccca cctcctcgaa cagcagcacc tcgtccgtca gcgccgcccg gggatcgacc
     4741 accgccggaa gcgggtcgta gtcgatctcg accaggtcgg cggcgtcctg gccctggtag
     4801 gactgctcgg tcagcacggc ggccaccggc tcgccgacga accgcacctt gtcggtggcc
     4861 aaccaggggc ggaccatgcc cgggttcgcg ccggcgaaca acagcgtcgg agccaggtcg
     4921 atgtcggcgc cggcgaatac cgcgacgaca ccgggcgcct cgcgggcggc ctcgacgtcg
     4981 acggcggtga tccgcgcatg cgcaagtggg gaccgcacga gcgtcaggtg cagcgcaccg
     5041 ttcagccgct cgtcggtcag gtcatctgta tatgtggcgc cccgggaaag gaacaccgga
     5101 tcctcggtgc ggaccacacg cgtgcccatg atgctcatcg aacgaaagcc tagctcgcag
     5161 gccacggtcc ggtgggacgg gatgacaaga tctcgggcac accctcaggc gcgttcacac
     5221 cctcggtgac caggcagttg caggaaagcc acgttcacgc agccaagcgc gacacgaacg
     5281 tggcttcccg gcgacaaggg gagatttcag gcgtccgaca gcggcaggta aacgcggccg
     5341 ccggcatctg tgaactcgtc cgacttctcc gacatcccgg cctcgattgc ctccacgctc
     5401 gtgagcccgt gcgcctctgc gtagtcccgg acgtcctgcg tgatgcgcat cgagcagaac
     5461 ttcggcccgc acatggagca gaagtgcgcg ctcttggccg gctccgccgg cagggtctcg
     5521 tcgtggaagg acagcgcggt gtccgggtcg agcgacaggt ggaactggtc gagccagcgg
     5581 aactcgaacc gcgccgccga cagggcgtcg tcacgtgcct gggcccgagg gtgctccttg
     5641 gccaggtcgg cggcatgcgc cgcgatcttg tacgtgatca cgccggtctt gacgtcgtcg
     5701 cggtccggca ggccgaggtg ttccttgggt gtgacgtaac acagcatggc ggtgccccgc
     5761 gcggcgatcg tggcggcacc gatggcgctg gtgatgtggt cgtagccggg tgcgatgtcg
     5821 gtggcgagcg ggccgagcgt gtagaacggc gcctcgccgc accactcctc ctccagccgc
     5881 acgttctccg cgatcttgtg cagcgggacg tggccggggc cctcgatcat cacctgcacg
     5941 tcgcgagcac gggcaaccgc cgtgagctcg ccgagggtcc gcagctcggc gagctgcgcc
     6001 tcgtcgttgg cgtcggcgat cgagccgggc cgcagcccgt cgccgaggga gaacgtcacg
     6061 tcgtaggtgc ggaggatgtc gcacagctcc tcgaaccgcg tgtagaggaa cgactcgcgg
     6121 tggtgcgcga ggcaccaggc ggccatgatc gacccgcccc gggagacgat gcccgtgacg
     6181 cgtttcgcgg tgagtgggac gtagcgcagc agcacgcccg cgtgcaccgt catgtagtcg
     6241 acgccctgct cgcactgctc gatcacggtg tcgcggtaca cctcccacga cagcgccgtc
     6301 gggtcgccgc ccaccttctc cagcgcctgg tagatcggca ccgtgccgat cgggacgggc
     6361 gagttccgca ggatccactc acgcgtctcg tggatgttct tgcccgtcga caggtccatg
     6421 acggtgtcgg cgccccagcg ggtcgcccac accatcttct ccacctcctc ctcgatcgag
     6481 gaggtcaccg ccgagttgcc gagattggcg ttgattttca ccaggaacgc cttcccgatg
     6541 atcatcggct cggcttcggg gtggcaccgg ttggccggga tcaccgcccg tcccctcgcg
     6601 acctcggacc gcaccagctc gacgtccacg ctctcccgga tcgcgatgaa ccgcatctcc
     6661 ggtgtgatga ccccggcccg cgcccaggcc agctgcgtgg cggcgccgcc gaccggctcc
     6721 ctggcggcga tccatggggc gcgcagggcc ggcaacccgt ccctgacctc gatcgtcacg
     6781 ctgtcgtcgg tgtactgccc tgacgtgtcg tagacgtcgt ggtgttcgcc ggtgccgagc
     6841 tcgatccggc ggaccgggac ccgcaggtcg tccggcccgt ccaggtacgc cttgtgtgag
     6901 cccctgatgg gaccggtggt gacctgaggc gcgaccgtac ggtcctcgag tgccgtcatg
     6961 gcaattcctt cctacgccgg cattacccgg acaggttctg cggtcggcgg cgtcgttcgg
     7021 ccgccctctc agcccgttcc accacgagct cccgcgtgtc atctgaagtt gtgctcaccg
     7081 gtcgagtgac cgggtgatca gggggtgggc tcctcccacc aggggcgcac cgcccacagc
     7141 ggcgacaccg gcccatggcc ggcgccgagc gggtacgagt gccggacggc ctccacgatg
     7201 tagcgcttgc cgagcgcaac ggcgtcgggc acggccattc cccgcgccag cccggacgcg
     7261 atggcggatg ccatgctgtc gccgccgcca tgggtgttgc cggtgtcgaa ccgcggtccc
     7321 ggcagctcgg tgaacgtgtg tccgtcgtag agcaggtcga cgcagccgtc ggtgtcctcg
     7381 tgcaggtgtc cgcccttgac cagcacgtac tgcggcccga ggccgtgcag caccttcgca
     7441 gcctcgtact gggcgctgcg gtcgtgcacg tcgatcccga ccagcagccg cacctcgtcg
     7501 aggttcgggg tgatgacggt ggcgcgcggg aagagcaacg cacggaaggc atcgagggcg
     7561 tcgtcggcca gcagttggtc gccgtgcatc gacgccgcaa ccgggtcgac cacgagcgga
     7621 gtggatccgg tgccgccgat cccgacccgg tcgcacgctc cggcgatcgc ctcgatgatc
     7681 ggccggctcg cgagcatgcc ggtcttcgcc gcgtccagcc cgatgtcgct ggccaccgcc
     7741 tcgatctgag ctgccacggt ctccggcggg atcggatgca cgcccgtgac gcccacggag
     7801 ttctgcaccg tgaccgcggt gaccgccacg cacccgtgca caccgcacgc cgcgaacgca
     7861 cgcagatcgg ccgggatgcc cgcgccccca ccggaatcag tgccggcgat cgtcatcacc
     7921 ctcggcggcg agacaccgac ggcgcgttcg ctcatgcttc cctcccgacg ccggcattac
     7981 ccg
//
LOCUS       DASRSC010000298         7255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4545425,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000298 DASRSC010000000
VERSION     DASRSC010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7255
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4545425"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="VFE39_14755"
     CDS             complement(<1..461)
                     /locus_tag="VFE39_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009152889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HET6259897.1"
                     /translation="MTTFPFSAVVGHDDLRLALLLNAVHPGVGGVLVRGEKGTAKSTA
                     VRALAALLPRLHVVAGCRFSCDPDAPDPECPDGPHPAVPAHSGQAESVVGSCPTDTRT
                     ARLVELPVGATEDRLVGSLDLERALSEGVRAYQPGLLADAHRGVLYVDEVNL"
     gene            complement(458..2056)
                     /locus_tag="VFE39_14760"
     CDS             complement(458..2056)
                     /locus_tag="VFE39_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HET6259898.1"
                     /translation="MGNDLRGALLVAGTTSDAGKSALVAGICRWLVRRGVAVAPFKAQ
                     NMSNNSVVTTDGGEIGRAQAMQAHACGLEPSVAFNPVLLKPGSDRSSQVVVRGRVDGT
                     VSARSYYARKAALLDVVGAELAGLRERYDVVVCEGAGSPAEINLRGSDIANMGLAQAA
                     GLPVVVVGDIDRGGVLAHLFGTLAVLDAADQARIAGFVINKFRGDLSLLTPGLDRLRE
                     LTGRPTLGVVPWVENIWLDAEDSLSAVADGVLGRPAPPHGSQWLHVAVVRLPRISNAT
                     DAEALACEPGVAVRYVTEPSRLLDADLVVLPGSKATAADLAWLRRTGLADAVLAHARA
                     GRLVLGICGGYQMLGRRIVDPHGVEGGVEGRDVEGLGLLDLEVAFDAEKHLGNPAGTA
                     WGEQVRGYEIHHGRVVRSGNPPLLHAAGPGEPAAGSVGAAERGDPEGSDAGVVIGTHW
                     HGLLENDGFRRVLLRRVAEQTGRSGFEPAPDTSFAAERDAQLDLLGDLVEEHIDTRAL
                     EHVISHGAPLDLPVVTSGLAGSSR"
     gene            2177..3016
                     /locus_tag="VFE39_14765"
     CDS             2177..3016
                     /locus_tag="VFE39_14765"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family cytochrome oxidase biogenesis
                     protein"
                     /protein_id="HET6259899.1"
                     /translation="MRFLLRPGWLAFIAVVVGFAIACYTLLAPWQFGREAQRDAEQQA
                     IDASYRTAPVPLAELLPAGRGVGPEVEWRQVTVSGTYLPEDEALVRLRVVDGKPAFEV
                     LTPFRTDDGRLVTVDRGSVTTPSGAQVPAFATAPSGNVTLTARLRMNETDPEARPVFE
                     SGGHRQLYAADSRPLATATGLDLEPGYLQLAADQPGVLSPLAVATDTGAAPFTNFSYA
                     LQWLTFGAIALFAMAYFVRLELLQRRGDRKADRTALRQALAGEDPDANPNETPLADRY
                     GRR"
     gene            3325..3810
                     /locus_tag="VFE39_14770"
     CDS             3325..3810
                     /locus_tag="VFE39_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010311185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6259900.1"
                     /translation="MRHKIIPNLWFDTEAEEAAAFYISVFGNSRILKVTHYTDAGPRP
                     AGTVMTVEFELDGQRFVGINGGPEFTFDEAVSFQINCETQEEVDYFWERLSEGGQEGP
                     CGWLKDRFGLSWQVVPTGLEELITDSDPARAQRAMQAMFGMGKLDIAALHSAADGVSA
                     T"
     gene            complement(3821..4786)
                     /locus_tag="VFE39_14775"
     CDS             complement(3821..4786)
                     /locus_tag="VFE39_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HET6259901.1"
                     /translation="MRPHAWVRPAGLLLGMVADAALGDPRRWHPVAGFGSVAAVLERR
                     CYSDRRVTGILHTAVLVVAATGLGVLAERAVAGRPLASVTLTGAATWAVLGGTSLSRH
                     GAALAAELTDGDLVAARSRLPSLCGRDPAVLDADGMTRAGTESMAENTSDAVVAPLLW
                     GAVAGVPGLLGYRAVNTLDAMVGYRSPRYRRFGWAAARLDDLVNLLPSRVAALLFAGL
                     APAVGGSPAAALAAWRRDASAHPSPNAGPVEAAVAGALGVGLGGRTVYPHGVEERPRL
                     GAGRAPSPVDLHRAARLSRLVGWATAGLAAMVASSAPEYRRCAST"
     gene            4844..5494
                     /locus_tag="VFE39_14780"
     CDS             4844..5494
                     /locus_tag="VFE39_14780"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET6259902.1"
                     /translation="MRTELDPVEFGRGPTYRLLTAVVVPRPIAWVSTRSTDGVDNLAP
                     HSFFTVSCVDPPMVQFTSVGRKDSLRNAQETGEFVVCLATEQLFEQVNATATDFPAGV
                     SEFSEVGLTPEPSAVVAPPRVVESPVALECRLHQTLELGDSTVVIGRVVHAAVTTDMF
                     DGDPQSGALPSIDKLRPLARLGRNEWSTIGDVLDITRISHKEWPGHYAGRSSPPTA"
     gene            complement(5461..5706)
                     /locus_tag="VFE39_14785"
     CDS             complement(5461..5706)
                     /locus_tag="VFE39_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259903.1"
                     /translation="MTQPRRPGAGPLAEVMAGMPEVWRRLLAAHVPDRMGRCTSCRTS
                     SGSGERWPCSLHRIATDAERVYGLRLGQAVGGEERPA"
     gene            complement(5923..>7255)
                     /locus_tag="VFE39_14790"
     CDS             complement(5923..>7255)
                     /locus_tag="VFE39_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6259904.1"
                     /translation="IPASTLLGPADLVDRVERGQAKHVVVRAADAPKFAGIEGGYTRI
                     AVGAPVEGWHSYSTAYAEPAEFTPDGVTPAGDPLLLYFTSGTTAKPKLVQHTQVSYPV
                     GHLSTMYWIGLEPGDVHLNISSPGWAKHAWSNVFAPWNAETTVFLYNYNRFDAPALMA
                     QMDRCGVTSFCAPPTVWRMLIQADLSVLRTPPKKVVGAGEPLNPEVIEQVEKAWGVQI
                     RDGFGQTESTVQIGNPPGQTVKPGSMGRPIPGYPIALIDPATGEKAEEGEICIDLSQR
                     PLGLMVGYAGDDKRNAEAMAGGYYHTGDVGRRDDNDYVTYVGRADDVFKASDYRISPF
                     ELESVLIEHPAVAEAAVVPSPDPVRLAVPKAYVVLAAGHEPTEETARSILEFARDNLA
                     SYKRIRRLEFGELPKTISGKIRRVELRGRETELHGADRGDGGPPPSGEFVL"
BASE COUNT         1034 a         2710 c         2523 g          988 t
ORIGIN      
        1 agattgacct cgtcgacgta gagcacgccg cgatgggcgt cggcgagcaa tcccggctgg
       61 tacgcccgca cgccctcgct cagcgcgcgc tccaggtcga gcgagccgac gagccggtcc
      121 tccgtcgctc cgacgggaag ctcgacgagc cgggccgtgc gggtatcggt cgggcaggac
      181 ccgaccacgg actcggcctg cccactgtgc gcgggtacgg ccgggtgcgg gccgtcgggg
      241 cactccggat cgggggcgtc ggggtcgcag ctgaaccggc aaccggccac aacgtgcagc
      301 cggggcagaa gcgccgccag cgcgcgcacg gccgtcgact tcgcggtgcc cttctccccg
      361 cgcaccagca ccccaccgac gccgggatgc acggcgttga gcaacagcgc gagccgcagg
      421 tcgtcgtggc ccacgacggc ggagaagggg aacgtggtca tcgggacgag cctgccagac
      481 cggacgtcac gaccgggagg tcgaggggcg caccgtgact gatcacatgt tcaagtgcgc
      541 gggtgtcgat gtgctcctcg acgaggtcac cgagcaggtc gagctgggca tcccgctccg
      601 cagcgaagct cgtgtccggc gcgggctcga acccggaacg gcccgtctgc tccgcgacgc
      661 gccgcagcag cacgcggcgg aatccgtcgt tctccaggag cccgtgccag tgggtcccga
      721 tgacgacgcc ggcgtcggat ccctctggat cgcctcgctc agcggctcct accgacccgg
      781 ccgccggttc gccgggacct gcggcgtgga gcagtggcgg gttgcccgag cgcaccacac
      841 ggccgtggtg gatctcgtag ccccgcacct gttcgcccca tgccgtaccc gccgggttgc
      901 ccaggtgctt ctcggcgtcg aaggccacct cgagatcgag caggccgagc ccctcgacgt
      961 cacggccctc gacgccgccc tccacaccgt gcggatcgac gatccggcgc ccgagcatct
     1021 gatagccgcc acagatcccg agcaccagcc gcccggcgcg ggcgtgcgcc agcaccgcgt
     1081 cggcgagccc ggtacggcgt agccacgcca ggtcggcggc cgtggctttc gaaccgggca
     1141 gcaccaccag gtcggcgtcc agcaaccggg acggctccgt cacgtaccgg acggcgacgc
     1201 ccggctcgca cgccagcgcc tccgcgtcgg tggcgttgga gatccgggga agccggacga
     1261 cggcgacgtg cagccactgc gacccgtgcg gcggcgccgg ccggccgagc acgccgtcgg
     1321 ccacggcgga cagcgagtcc tccgcgtcga gccagatatt ctcgacccac ggaacgacac
     1381 ccaacgtcgg acgtccggtg agctcacgga gccggtcgag gccgggggtg agcagggaga
     1441 ggtcaccgcg gaacttgttg atcacgaagc ccgcgatgcg cgcctggtcg gccgcgtcca
     1501 gcacggcgag ggtgccgaac agatgggcga ggacgccgcc gcggtcgatg tcgccgacca
     1561 ccaccaccgg cagaccggca gcctgcgcga gccccatgtt cgcgatgtcg ctgccccgga
     1621 ggttgatctc cgcaggcgag cccgcgccct cgcagaccac cacgtcgtag cgctcgcgca
     1681 gcccggcgag ctccgccccg acgacatcga gcagggccgc cttccgcgcg tagtaggacc
     1741 gcgcgctgac cgtgccgtcg acgcgcccgc gcaccacgac ctgcgagctg cggtcgctcc
     1801 ccggcttcaa cagcaccggg ttgaacgcga cgctgggctc cagcccgcag gcgtgcgcct
     1861 gcatcgcctg agcccggcct atctcgccac cgtcggtcgt gaccaccgag ttgttcgaca
     1921 tgttctgcgc cttgaacggc gccacggcca ccccgcgacg tacgagccag cgacagatcc
     1981 cggcgaccag ggcactcttg ccggcgtccg acgtcgtgcc cgccaccagc agcgcaccac
     2041 gtagatcgtt ccccacgctg atcctccccg gggccgcgga cacaccctac gtcgccgggg
     2101 gccctgtttc gtcagtccgg ctgctccggt cggtggcggg cgcgctctcg gacggccgag
     2161 cgcctaccgt ggccctgtgc ggtttctcct acgtcccggt tggctcgcct tcatcgcggt
     2221 ggtcgtcggg ttcgcgatcg cctgctacac gctgctcgcc ccgtggcagt tcggccgcga
     2281 agcccagcgc gacgccgagc agcaggcgat cgacgcctcc taccgcaccg cgcccgtgcc
     2341 gctcgccgag ctcctgcccg ccggccgcgg cgtaggtccc gaggtggagt ggcggcaggt
     2401 cacggtgtcg ggtacgtacc tgccagagga cgaggcgctc gtccggcttc gggtggtgga
     2461 cggcaagccc gcgttcgagg tgctgacccc gttccgcacg gacgacgggc ggctggtgac
     2521 cgtggaccga gggtccgtca ccacgcccag cggcgcccag gtacccgcct tcgcgacagc
     2581 gccgagtggc aacgtcacgc tgacggctcg cctgcggatg aacgagactg acccggaggc
     2641 ccgccccgtg ttcgagtcag gcggacatcg ccagctctac gcggccgact ctcgcccact
     2701 cgccactgcc accgggctcg acctggagcc cggctacctg cagttggcgg ccgaccagcc
     2761 cggcgtcctc agcccgctcg ccgtggcgac ggacacgggc gcggccccgt tcacgaactt
     2821 ctcctacgcc ctgcagtggc tcacgttcgg tgcgatcgcg ctcttcgcca tggcctactt
     2881 cgtgcggctg gagctgctgc aacgcagagg cgaccggaag gccgaccgca ccgccctccg
     2941 ccaggcgctc gccggcgagg acccggacgc gaacccgaac gagaccccgc tcgccgaccg
     3001 ctacggccgc cgctagcccg gtcagcaggg cgatccgagt tcgggttgtc gaccccgtcg
     3061 gtcagcttcg gccgccgctg cagcgccgat gcccggttct ggagcgcagc aatccagcgg
     3121 tgatcaaagc accgatgtcg cgtcacaaat ctgctagcga ctaccgcgcc gggcggcggc
     3181 ggggcggggc gccaaatgag ctgggagggg cgcggcattc gtccggtcgg gcgaagggcg
     3241 ctgagttctc cgccgtcgca gggtccactc cgtgcagcaa tcgacgggcc ctcggtcccg
     3301 cacccgacga gaggacgcag cgccatgcgg cacaagatca tccccaacct gtggttcgac
     3361 accgaggccg aggaggcagc ggccttctac atctccgtct tcggcaactc gcgcatcctg
     3421 aaggtcacgc actacaccga cgccgggccc cgtccggccg ggacggtgat gaccgtggag
     3481 ttcgagctgg acggtcagcg gttcgtcggc atcaacggag gcccggagtt cacgttcgac
     3541 gaagccgtct ccttccagat caactgcgaa acccaggagg aggtggacta cttctgggag
     3601 cggctgtccg agggcgggca ggagggtcca tgtggctggc tcaaggaccg gttcggcctg
     3661 tcgtggcagg tcgttccgac gggactggag gagttgatca ccgattcgga tccggcgcgc
     3721 gcccagcgcg cgatgcaggc gatgttcggg atgggcaagc tcgacatcgc tgcgctgcac
     3781 agcgcagccg acggcgtgtc ggcgacctga cagacccgcg tcaggtggag gcgcaccgcc
     3841 ggtactcggg cgctgaggac gccaccatcg ccgccagccc cgcggtcgcc cagccgacca
     3901 gccgggacag tcgcgcggcc cggtgcaggt cgacaggcga cggggcgcgg cctgcaccga
     3961 ggcgcggacg ctcctccacc ccgtgcgggt agacggtgcg ccccccgagc ccgacgccca
     4021 gcgcgcctgc cacggccgcc tccaccgggc cggcgttcgg gctgggatgg gcggaggcgt
     4081 cccggcgcca cgccgccagc gctgcggcgg gcgagccgcc gacggcgggc gcgagtccgg
     4141 cgaagagcag cgccgccacc cgcgatggca gcaggttgac caggtcgtcg aggcgggcgg
     4201 ctgcccatcc gaagcgccgg tagcggggcg accggtagcc gaccatcgca tcgagggtgt
     4261 tcacggcgcg gtagccgagc agccccggca cgcccgccac cgcgccccac agcaacgggg
     4321 caaccaccgc gtcggacgtg ttctcggcca tcgactccgt gccggcccgc gtcatcccgt
     4381 cggcgtcgag cacggcgggg tcgcggccgc agagcgaggg cagccgtgac ctggctgcga
     4441 ccaggtcgcc gtcggtgagc tccgcggcga gcgcagcgcc gtgccgggag agcgacgtgc
     4501 cgccgagcac ggcccacgtc gccgcgccgg tgagcgtcac ggaggccagc ggacggccgg
     4561 ccacggctcg ctcggcgagc acccccagcc cggtggccgc tacgacgagt acggcggtgt
     4621 gcaggatgcc ggtcacccgc cggtcgctgt agcagcgccg ctccagcacg gcggccaccg
     4681 acccgaaccc ggccaccgga tgccaccggc gggggtcgcc cagtgccgcg tccgcgacca
     4741 tccccaacag cagtccggcc ggccggaccc atgcgtgcgg tctcacgccg gcgacggtac
     4801 ggcgggaccc ggtgccgcct tccctgcgag ggatgattgc tgcatgcgca ccgaactgga
     4861 tcccgtcgag ttcggccgag gaccgaccta tcggctgctg accgcggtcg tcgtgccccg
     4921 cccgatcgcc tgggtgtcca cccgctccac cgacggcgtc gacaacctgg ctccccattc
     4981 cttcttcacg gtctcctgcg tggacccgcc gatggtccag ttcacgagcg tcggcaggaa
     5041 ggactcactg cgcaacgccc aggagaccgg cgagttcgtc gtgtgcctcg cgaccgaaca
     5101 gctcttcgag caggtcaacg ccaccgcgac cgacttcccc gccggggtca gcgagttctc
     5161 ggaggtgggc ctgacacccg agccgagcgc tgtcgtggcg ccgccgcggg tggtcgagtc
     5221 accggtggcg ctggagtgcc ggctgcacca gaccctcgaa ctcggcgact cgacggtcgt
     5281 catcggccgc gtcgtgcacg cggcggtcac gaccgacatg ttcgacggcg acccgcagag
     5341 tggggcgctg ccctcgatcg acaagctgcg cccgctcgcc cggctcggac gcaacgagtg
     5401 gtcgacgatc ggcgacgtgc tcgacatcac ccggatctcg cacaaggaat ggccggggca
     5461 ctacgccggg cgctcctccc cgcccaccgc ctgaccgagt cgcagcccgt acacccgctc
     5521 tgcgtccgtg gcgatccggt gcagcgagca gggccagcgc tcgcccgagc cgctggaggt
     5581 gcggcaggag gtgcaccggc ccatccggtc ggggacgtgc gcggccagca gccggcgcca
     5641 gacctccggc atgcccgcca tcacctccgc cagcggcccc gcacccggcc tacgtggttg
     5701 cgtcacggtc ccctcaacct gcttcgccac accggcgatt cagatctgca cggactgtcg
     5761 ccgagcggca cccggtgtgc gaccggcgcc acccgatcgg cgtctcccgc gcgggccaga
     5821 cccggtcacc ccatgacgct ctgccgctcg tcgcccggca ttcgagtgag ggcgacggcg
     5881 acggtgacac ggaaatctac ccggcgctcg aggcgctcgg gctcacagga cgaactcgcc
     5941 gcttggcggc ggcccgccgt ccccgcggtc agcaccgtgc agctccgtct cccgtcctcg
     6001 cagctccacc ctcctgatct tgcccgagat cgtcttcggt agttcgccga actcgaggcg
     6061 tcggatccgc ttgtacgacg ccaggttgtc gcgggcgaac tccaggatcg accgcgccgt
     6121 ctcctcggtc ggctcgtgcc cggcggccag caccacgtac gccttcggca cggcgaggcg
     6181 caccgggtcg ggggacggga ccaccgccgc ctcggccacc gccgggtgct cgatgagcac
     6241 gctctccagc tcgaacgggc tgatccggta gtccgacgcc ttgaacacgt cgtcggcgcg
     6301 cccgacgtag gtgacgtagt cgttgtcgtc gcggcggccg acgtcaccag tgtggtagta
     6361 cccgcccgcc atcgcctcgg cgtttcgctt gtcgtcaccc gcgtagccga ccatcagccc
     6421 gagcggccgc tgggagaggt cgatgcagat ctccccctcc tcggccttct cccccgtcgc
     6481 ggggtcgatc agtgcgatgg ggtagccggg gatggggcgt cccatcgaac ccggcttgac
     6541 ggtctgcccc ggcgggttgc cgatctgcac ggtgctctcg gtctggccga aaccgtcccg
     6601 gatctgcacg ccccaggcct tctcgacctg ctcgatcacc tcggggttca gcggctcccc
     6661 cgcgccgacg accttcttcg gcggggtccg cagcacgctc aggtcggcct ggatcagcat
     6721 ccgccagacc gtgggcggcg cgcagaagct ggtcacgccg cagcggtcca tctgcgccat
     6781 cagggccggc gcgtcgaacc ggttgtagtt gtacaggaac accgtggtct cggcgttcca
     6841 tggcgcgaac acgttgctcc acgcgtgttt cgcccagccg ggcgaggaga tgttcaggtg
     6901 gacatcgccc ggctccaggc cgatccagta cattgtggac agatggccga ccgggtacga
     6961 cacctgcgtg tgctgcacca gcttcggctt cgccgtggtg ccggaggtga agtacagcag
     7021 cagcgggtcg cccgcggggg tgacaccgtc cggggtgaac tccgcgggct cggcgtaggc
     7081 ggtggagtac gagtgccagc cctccaccgg cgcgccgacc gcgatccgcg tgtagccgcc
     7141 ctcgatcccg gcgaacttcg gcgcgtcggc ggcgcggacc acgacgtgct tggcctggcc
     7201 gcgttccacc cggtcgacga ggtcggccgg gccgagcaag gtggacgccg ggatg
//
LOCUS       DASRSC010000299        14189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_452548,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000299 DASRSC010000000
VERSION     DASRSC010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 14189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14189
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452548"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2018
                     /locus_tag="VFE39_14795"
     CDS             <1..2018
                     /locus_tag="VFE39_14795"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HET6259905.1"
                     /translation="GLRITGPVGEFTVRAPAVLPAGLPDSIDGVVLVAVKGHHTAAAA
                     EVLRDRLAPTALVVSMQNGLTTDLLSEVVGRERLLACFVNFGADVLEPGVVMQGNVGT
                     FRIGELDGRMTDRLREIADALPYAEPTDNILGYLWAKEAYGAMLFAGAVSDLSIADSL
                     EDPRFRPLMLAIAREVLAQAPVTPMPFDGFDPEDLLGSLTRLVAFNRGSAKSHSGIYR
                     DLAVRNRRTEVEIQLDPLTGPLTGYVAELIRAIERGERVCEVANLELLAAYERLERLG
                     RPLHAVVAALPAPQRAKAGPLHGMPVAVKDIIDVAGSPRGNGNPHDMAGPPAGSDAPA
                     VAALRAAGADVFALASLLEYAAGAPHPDLPEAMNPARPDRTAGGSSGGSAALVAAGVC
                     PAALGTDTGGSIRIPAAYCGIVGLKPTSGLVSAEGVTPLSPTLDHVGVLAASVADAAA
                     VLAALTGPGAPPLEEAPARPYRLGVLTDQLADPRLDPELAAITRAALDRLVVAGLVLA
                     DSDGAVLTELAECLEDILLVEAWQVHGRAISGPDADPELFGATTLRLLRSAADVEPRR
                     REAALARRAELLPAAEGLLDGVDVLVGPATPYAAPERTPPVDTPEGEIEGIFSGPYNV
                     TGQPAIVVPCGLTAEGLPVGLQLAARCGDDAGLLRVAAAVEAALAAP"
     gene            complement(2120..3043)
                     /locus_tag="VFE39_14800"
     CDS             complement(2120..3043)
                     /locus_tag="VFE39_14800"
                     /inference="COORDINATES: protein motif:HMM:NF016817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HET6259906.1"
                     /translation="MQELIVPLTDGTLSVPYVDVTGASPGPHLTVLAGIHGTEYTSIA
                     AVREFARGLDPAWICGRITAVPIVNLPAFWERSPFVVPLDGKNLNRSFPGDPAGTAAE
                     VLAHHVTESFIRPTDYLLDLHAGDLPEALEPFVLYDESPVEEESRRLARAYGLGHLVR
                     QARAGRAVGGSTSAVAADLGIPSITAEAGQNGILDRASIDRHLTGLAGVTRLLGIDNR
                     LSPGNGVTAPPVEHDGWVWLRTSTSGWWEPAVAVGTDVDAGDLLGTVSGLLGDDRTEI
                     RAPEAGVPLFVTSSPAVTADGLLMGLARAAR"
     gene            complement(3142..4197)
                     /locus_tag="VFE39_14805"
     CDS             complement(3142..4197)
                     /locus_tag="VFE39_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6259907.1"
                     /translation="MTAPTKTGPTTTAGRAIPTQRTDSEHQEATSERPELSAEDLDAQ
                     SPAADLVRVYLNGIGKTALLSAAQEVELAKRIEAGVFAQHVLDAAVEEGTDLESRYAA
                     DLRAVVRDGMRARNHLLEANLRLVVSLAKRYTGRGMPLLDLIQEGNLGLIRAVEKFDY
                     AKGFKFSTYATWWIRQAITRGMADQARTIRLPVHLVEQVNKLARIKRDLHQKLGRDAT
                     HEELATESGIAEEKIADLLDHARDPVSLDMPVGSEEEAPLGDFIEDGEATDAETTVIS
                     HLLHDDLRRVLATLDDREQQVIRMRYGLDDGQPCTLDQIGRRFGLSRERVRQIEREVM
                     AKLRIGERADRLRAYAS"
     gene            complement(4343..5662)
                     /locus_tag="VFE39_14810"
     CDS             complement(4343..5662)
                     /locus_tag="VFE39_14810"
                     /inference="COORDINATES: protein motif:HMM:NF022783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6259908.1"
                     /translation="MTATSGTGTAVARTADIMAALTHLTGYNAAAAMSSLTEMTRVTG
                     AVWTDALTRSATPLDIAARNLRWWRAMRHRNEPQWHLPNEIVLSTPFAHLRDFTHRDG
                     SDNSSDNESDVVPTLVLPPQAGHSSSVIDFSPTQSQLAVITAAGLVRLWALEWRPATA
                     ATAHVTITDYLDVIDRSVELMGGRANLIGDCQGGWLAAIYAALHPERVHTLTLAGAPI
                     DFHAGESVIASSTRALTTAFGLAPYRALVALGGGNMPGKAVLGNFIAIQPQSEISRQL
                     QLLENIDDAAHVQRYRVFEDWFKNTQDIPGAFYLWLVEHLFWGNELISGDLVVDGRRA
                     DLANITCPLFLLAGSTDHITPPPQLFAAADAVGTPQDQIWCRTAEGGHLGLFMGRDAL
                     RTDWPPLLEIVRALSEPQPAGAAPRRRRGRTADVVADPGGTPRVPAP"
     gene            5841..6260
                     /locus_tag="VFE39_14815"
     CDS             5841..6260
                     /locus_tag="VFE39_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6259909.1"
                     /translation="MCAARPADDDATADGSPAAGWSAQINALGDFIRAQRQLANLSLR
                     EMASMTSVSNAYLSQVERGLHQPSLKVLHSIADALQLSTEQLLGQAGWVTGTGAATAS
                     AESGGTEDAIRKDPRLSPEQRIALLGVYRSFVAGATE"
     gene            6357..6755
                     /locus_tag="VFE39_14820"
     CDS             6357..6755
                     /locus_tag="VFE39_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259910.1"
                     /translation="MTASTDQFADIATRTQEAVTTAVRTWADTVQTFTGSITGSRPAL
                     PDAQVVVDRYFDFAEQVLENQRRLAKSVLTAGAQAAESVTEQAARTAESVAARTVNAT
                     ETVATKAADVTKAAGERTAASARAAQNASK"
     gene            complement(6867..7292)
                     /gene="dtd"
                     /locus_tag="VFE39_14825"
     CDS             complement(6867..7292)
                     /gene="dtd"
                     /locus_tag="VFE39_14825"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HET6259911.1"
                     /translation="MRAVAARVSRASVHVDGEVVGQIDRLGLLVLLGVHRDDSPEAAP
                     AMARKLHELRILPEERSCAEEDAPLLVISQFTLYGDTRKGRRPSWSAAARPEHAEPLV
                     KAVVAALRARGAEVATGVFGARMAVESVNDGPFTVIVEV"
     gene            complement(7289..8818)
                     /locus_tag="VFE39_14830"
     CDS             complement(7289..8818)
                     /locus_tag="VFE39_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HET6259912.1"
                     /translation="MRPHLTPATADSLRTALLRAGYTTDGVLALLGPAAHAALGRGEV
                     EPAFRAARDGGELGVLVRLLLLGSVEPHAAVTAALAPLAPADAAAAGLLRAAGDGWTA
                     ALDLRPYGDTSDSADHMDGDTGGDWWVLSDLDQRRQERDHVTGVGAASLTLAAATARR
                     PVGSLLDLGTGCGVQALHATRHAGAVTGTDLAPRALAMARATFALNDLDVELLDGPWL
                     EPVEGRRFDQIVSNPPFVPGPARVDYVYRDSGQDGDAALAALLGALPEHLEPGGVAQL
                     LGSWLHVRGEDWPDRVRSWLPAGVDAWIVQREVADPALHVGTWQRDAGLDLTAPAVRE
                     QAGAWLDWLDRERVEGVGFGFVTLRRPRGGDPLVVFEDMPGDIEDPLGPEVEGWLDRV
                     DWLRAHTGDEALLGARLTVAPSVVLERYAEAGADGWAEVGSAVARRDGPRWRHEVDGP
                     AAALLAGCQGALPLGELVELLSFAHDRPTGELVAAALPAVREFVRHGVLVPAGELGQG
                     R"
     gene            8863..9462
                     /locus_tag="VFE39_14835"
     CDS             8863..9462
                     /locus_tag="VFE39_14835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HET6259913.1"
                     /translation="MPLCLSQDTDADALLDRDPFALLVGMLLDQQFPMERAFAGPALL
                     AQRLGRTELDARELASYDPDALVEIFRGPPAVHRYPGSMAGRVQALAAAVVDQYDGDV
                     TLIWRDVEDGRELFRRLNALPGFGTQKAQIFTALLGKQRGVTPAGWREAAGGYGQEGS
                     YRSVADVVDVASLGRVREYKQAQKAAAKAGSKAAAPAKG"
     gene            9492..9923
                     /locus_tag="VFE39_14840"
     CDS             9492..9923
                     /locus_tag="VFE39_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6259914.1"
                     /translation="MHQVPPARRDVRVIDPDQVCDAAAVLADGAQLQHSAAVLDALGD
                     VHRLSILLALHHAGDLCVSDLAFAVGMSDSAVSHALRLLRAHGMVSAQREGRLVRYRV
                     VGELTTRLLDVVSEGVVGVDVVLHAHGAAGAHGEAVAVVND"
     gene            10101..10418
                     /locus_tag="VFE39_14845"
     CDS             10101..10418
                     /locus_tag="VFE39_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3099 domain-containing protein"
                     /protein_id="HET6259915.1"
                     /translation="MADPQRSGVEPVLITDAARSYEEELATRKRRYKIMMGMRIPFMV
                     LAAVFYQIPWLAVTLLVLSIPLPWIAVLIANDRLPIKAEAVSRYEAERRQIEAREHPV
                     IEG"
     gene            complement(10405..11295)
                     /locus_tag="VFE39_14850"
     CDS             complement(10405..11295)
                     /locus_tag="VFE39_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HET6259916.1"
                     /translation="MPGTRRPRVVVVGDVAVDVLVAPSGPAVHGADVPARIRTGAGGA
                     GANTAAWLAHLGADVTLVARVGEDAAGSAAAADLADAGVLPALTVDPDVPTCTVVVLL
                     DEGERTMLSDRGAAARLDPADLPALDGADHLHLSGYVLLDPSSRPAGLAALAAARAAG
                     LSTSVDPQAAPALTPEFREWVRGIDLLLPNTDELAALGGSETELLVEMGTAGAVCVTE
                     GARGARWVDHRGRWEVTAPGVDVVDPTGAGDAFDAGLLVAWLRGAGPADALRAGCAAG
                     AAAVGGLGARPTGRRGVSPR"
     gene            complement(11313..12272)
                     /locus_tag="VFE39_14855"
     CDS             complement(11313..12272)
                     /locus_tag="VFE39_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HET6259917.1"
                     /translation="MPHVPVPVSPPALPPPTPIVMSPEVADAVATKRPVVALESTLLA
                     HGLPSPHNRAAADDLETAVRAHGAVPATVAVLDGVPRVGLSGPELDQVCGGGLVKLSA
                     RDLGVAVALGRDGATTVAATAALAHAVGVALFATGGLGGVHRGARDTWDVSADLAALA
                     ATGVFVVCSGVKSILDVPATLEVLETGSVPVIGYRTDAFPGFYRRDSGHPVPWRVDTP
                     TEAAAVWRAHRSLGSPSGMVLAKPVPEADELNAGLHDRLLEEGTALLARRRITGKDVT
                     PALLEHFHAGSGGASLRTNIALVRANAELAAQVAVALAARPRP"
     gene            12283..12531
                     /locus_tag="VFE39_14860"
     CDS             12283..12531
                     /locus_tag="VFE39_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005465298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3039 domain-containing protein"
                     /protein_id="HET6259918.1"
                     /translation="MATQVLPDVETRPEGTDTTGNDEPKMFHYVKKNKIAESAVLGNM
                     VVALCGETFPVTRSPKPGSPVCPDCKKIYDSLRPGGDD"
     gene            12543..13205
                     /locus_tag="VFE39_14865"
     CDS             12543..13205
                     /locus_tag="VFE39_14865"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6259919.1"
                     /translation="MSTVPMRSVLRGWLYDRLVAGMTTDWYREVLIRLPDGARLLDVG
                     IGTGAALARCADVVRAKRLDVVGLDIDTDYLERCRAEIAGAQLSAQVSPVLSSVYDHH
                     GGPYDAVYFSASLMLLPDPVAAIVHVGGQLAPDGRLFATQTFHHRRSPLLERAKPLVR
                     LVTTIEFGRVTYEDDFRRAFAAAGMELMELTTMSSTRHSSYRLAVARVSDHGNGRSTR
                     AA"
     gene            complement(13218..>14189)
                     /locus_tag="VFE39_14870"
     CDS             complement(13218..>14189)
                     /locus_tag="VFE39_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6259920.1"
                     /translation="PGRSIVEHLVRGGQQVFLLSWRNPGTEHATWDLDTYGRAVLDAM
                     DACEHVARTDRMSMMAFGSGGTITAMLLAHLAEIGAQERVASVTFVASVLDHEEAGTA
                     GATLIPKARAAMAASQRTGYLDGRVLAEAHAWLRPADLVWPYWVHNYLGAQSPPASDV
                     MFWLADTVRLPARLHRDLVTIALDNPLVTPGACGMLGTAVDLSKVDRDVYVVAAITGR
                     PSTWRSCYRTTLLLGGTSRFVLGGSTASVVCPPGEATASFRVADTTPAEAERWLAGAR
                     AVAGSWWSDHLAWLDERSGPLRDAPPELGGRGMHAVAPAPGEYVLQR"
BASE COUNT         1973 a         5103 c         5226 g         1887 t
ORIGIN      
        1 agggcttgcg gatcaccggc ccggtgggtg agttcacggt ccgagcgccc gcggtgctcc
       61 ccgccggcct cccggattcg atcgacgggg tggtgctggt ggccgtcaag gggcaccaca
      121 ccgccgcggc cgccgaggtg ctgcgcgacc ggctggcccc caccgcgctc gtcgtgtcga
      181 tgcagaacgg tctgaccacg gacctgctgt ccgaggtggt cggccgcgag cggctcctcg
      241 cctgcttcgt caacttcggc gccgacgtgc tggagcccgg cgtcgtgatg cagggcaacg
      301 tcggaacgtt ccggatcggt gagctggacg ggcggatgac cgaccggctg cgggagatcg
      361 ccgacgcgct gccctacgcg gagccgaccg acaacatcct gggctacctc tgggccaagg
      421 aggcctacgg cgccatgctg ttcgccggtg ccgtgtcgga cctgtcgatc gcagactccc
      481 tggaggaccc gcggttccga ccgttgatgc tggcgatcgc ccgcgaggtg ctcgcccagg
      541 ccccggtcac cccgatgccc ttcgacggct tcgatcccga ggatctgctg ggatcactga
      601 ccaggctcgt cgcgttcaac cggggcagcg ccaagagcca cagcgggatc taccgggacc
      661 tcgccgtgcg caaccgcagg accgaggtcg agatccagct cgaccccctc actggtccgc
      721 tcaccggcta cgtcgccgag ctgatccgcg caatcgagcg cggcgagcgc gtatgcgagg
      781 ttgcgaatct ggagctgctc gccgcctacg agcggctgga gcggctcggc cgtccgttgc
      841 acgccgtagt ggccgcgctg cccgcgccgc agcgggccaa ggcggggccg ctgcacggga
      901 tgccggtcgc ggtgaaggac atcatcgacg tggccgggag cccgcggggc aatggcaacc
      961 cgcacgacat ggccggcccg cccgccgggt cggacgctcc ggcggtggcc gcgctgcggg
     1021 cggccggcgc cgacgtcttc gcgctggcct cgttgctcga gtacgcggcg ggcgcccccc
     1081 atccggacct gccggaggcg atgaaccccg cgcgaccgga ccgaaccgca ggcggatcga
     1141 gcggtgggtc ggcggcactg gtcgccgccg gcgtctgccc ggccgcgctg ggcaccgaca
     1201 ccggggggtc gatccgcatc cccgcggcat actgcgggat cgtcggcctg aagccgacct
     1261 ccggcctggt cagcgccgag ggtgtcactc cgctctcgcc gacgctggac catgtcggag
     1321 tgctggcggc gtccgttgca gacgccgccg cggtgctggc ggcgctgacc gggcccggcg
     1381 cgccgccgct ggaggaggca ccggcgcggc cctaccggct cggggtgctg actgatcagc
     1441 tcgccgaccc gcggctggac cccgagctcg cggcgatcac ccgggcggcg ctggaccggc
     1501 tcgtcgtggc cggcctggtg ctggccgaca gtgacggggc ggtgctgacc gagctggcag
     1561 agtgcctgga ggacatcctg ctggtcgagg cctggcaggt gcacggccgg gcgatcagcg
     1621 gtccggacgc ggaccccgag ctgttcggag ccacgacgtt acggttgctc cgcagcgcgg
     1681 cggacgtcga gccgcggcga cgggaggccg cgctggcgcg gcgggccgag ctgcttcctg
     1741 cggccgaggg gctcctcgac ggggtggacg tcctggtcgg gccggccacg ccctacgcgg
     1801 ccccggagcg gaccccgccg gtggacacgc cggagggaga gatcgagggg atcttcagcg
     1861 gtccgtacaa cgtcaccggg caaccggcga tcgtcgtgcc gtgcgggctg accgccgagg
     1921 ggctgcctgt cgggctgcag ctggcggcgc ggtgcggcga cgacgccggc ctgctgcggg
     1981 tggccgcggc cgtcgaggct gcgctcgcgg cgccctgagc aggcgcggcg cgagcaccgt
     2041 ggtcaccgtg cggggaggcc ggtgctcgcc gtcggccagc aagccggaag agccgttctc
     2101 acgcacgcgt gggaacggct cagcgggccg cgcgggcgag gcccatcagc aggccgtcgg
     2161 cggtgacggc cgggctgctg gtcacgaaca acgggacgcc cgcctcgggc gcccggatct
     2221 cggtccggtc gtcgccgagc aacccgctga ccgtgccgag taggtcgccg gcatcgacgt
     2281 cggttccgac ggccacggca ggttcccacc aaccggacgt gctcgtacgc agccacaccc
     2341 agccgtcgtg ctcgacgggt ggagcggtca ccccgttgcc cggagagagt cgattgtcga
     2401 tcccgagcag gcgggtgacg cccgcgagac ccgtgagatg gcggtcgatg ctcgcgcggt
     2461 cgaggatgcc gttctggccc gcttcggcgg tgatcgaggg gatcccgagg tcggccgcga
     2521 ccgcggaggt gctgccgccg accgcgcgcc cggcccgagc ctgccggacc aggtggccga
     2581 ggccataggc gcgggccaac cggcgggact cttcctcgac gggggactcg tcgtagagga
     2641 cgaagggctc cagtgcctcg ggaagatcgc cggcgtgcag gtccagcagg tagtccgtgg
     2701 gccggatgaa ggactcggtg acgtggtgcg ccagcacctc cgccgccgtt ccggcgggat
     2761 ccccggggaa gctgcggttc aggttcttcc catcgagcgg gacgacgaac ggcgagcgtt
     2821 cccagaaagc gggcaggttc acgatcggca ccgccgtgat gcgcccgcag atccacgcgg
     2881 ggtcgagacc gcgggcgaac tcgcggacag cggcgatgga ggtgtactcg gtgccgtgta
     2941 tgccagcgag caccgtgagg tgtggaccgg gcgacgcacc cgtcacgtca acgtacggga
     3001 cggacagtgt gccgtcggtg agcgggacga tcaactcctg cacaccgtgc actgtgctcg
     3061 cccggccacc gtcgaggaaa ccgacctgcc gcgagtgtgc gcggtatcac cacacacggc
     3121 cgcgcccgcg acgggcggcg atcagctcgc gtaggcgcgc agccggtcgg cccgctcccc
     3181 gatgcgaagc ttggccatca cctcgcgctc gatctgccgg acgcgctcgc gggagagccc
     3241 gaagcggcgg ccgatctggt cgagcgtgca gggctgcccg tcgtcgaggc cgtatcgcat
     3301 ccggatgacc tgctgctcgc ggtcgtccag ggtggccagg acccgccgca ggtcgtcgtg
     3361 cagcaggtgc gagatcaccg tggtctcggc gtcggtggcc tcgccgtcct cgatgaagtc
     3421 gccgagcggc gcctcctcct cggaccccac cggcatgtcc aggctcaccg ggtcgcgggc
     3481 gtggtcgagc aggtcggcga tcttctcctc cgcgatgccc gactcggtcg ccagctcctc
     3541 gtgggtggcg tcgcgtccga gcttctggtg caggtcgcgc ttgatccggg ccagcttgtt
     3601 gacctgctcc acgaggtgca cgggcagcct gatcgtgcgg gcctggtcgg ccatgccgcg
     3661 cgtgatcgcc tgccggatcc accaggtggc gtaggtggag aacttgaagc ccttggcgta
     3721 gtcgaacttc tcgaccgcac ggatcagacc caggttgccc tcctggatca ggtccagcag
     3781 cggcatgcct cggccggtgt agcgcttcgc cagcgagacc acgagccgga ggttggcctc
     3841 caacaggtgg ttgcgggcgc gcatgccgtc gcggaccacg gcccgcaggt cggctgcgta
     3901 ccggctctcc aggtcggtgc cctcttcgac ggccgcgtcg agtacgtgct gggcgaagac
     3961 cccggcctcg atccgcttgg ccagctcgac ctcttgcgcc gcagagagca gagcggtctt
     4021 gccgataccg ttcaggtaca cgcgaaccag gtcggccgcc gggctctgag cgtcgaggtc
     4081 ctctgcggac agctctggac gctcggaggt ggcctcctgg tgctcggagt cggtacgctg
     4141 cgtcgggatg gctcggccgg ccgtagttgt cggcccggtc ttggtgggcg ctgtcatcat
     4201 tcctcccctg ccgcggcccg ggcggggcga tggagactcc cgtcgcccgg ggctgtcacg
     4261 tgtttgaacg cctgcaagcg gcagaacgtt cccgcgagcg ccaggggtgc cgggtattct
     4321 cagcgcctcc tcagtcacgt cgtcaggggg cgggcactcg cggcgtaccg ccgggatcag
     4381 cgacgacgtc ggcggtccgt cctctgcggc gcctcggcgc ggcaccggcg ggctgcggct
     4441 ccgacagcgc gcgcacgatc tccagcaggg gcggccaatc ggtccgcagc gcgtcgcgac
     4501 ccatgaacag gccgaggtgc ccgccctccg cggtacggca ccagatctgg tcctgcggcg
     4561 tgcctacggc gtcggccgcc gcgaagagct gcggtggcgg cgtgatgtgg tcggtcgatc
     4621 cggcgagcag gaacagcggg caggtgatgt tcgccagatc ggcgcggcgg ccgtcgacga
     4681 cgaggtcgcc ggagatgagc tcgttgcccc agaacaggtg ctcgaccagc cacagataga
     4741 aggcgccggg gatgtcctgg gtgttcttga accagtcctc gaagacgcgg tagcgctgga
     4801 cgtgtgcggc gtcgtcgatg ttctccagca gttgcagctg gcgggagatc tccgactgcg
     4861 gctggatcgc gatgaagttc cccagtacgg ccttgcccgg catgttgcca ccccccagcg
     4921 cgacgagcgc ccggtagggg gcgagaccga acgcggtggt gagcgcccgt gtcgaggacg
     4981 cgatgaccga ctccccggcg tggaagtcga tcggagcgcc tgccagggtc agggtgtgca
     5041 cacgctcggg gtgcagcgcc gcatagatcg cggcgagcca gccgccctgg cagtcgccga
     5101 tgaggttggc gcgcccgccc atcagctcga cggagcggtc gatgacgtcg aggtagtcgg
     5161 tgatcgtgac gtgggccgtc gccgccgtcg cggggcgcca ctcgagcgcc cagagccgga
     5221 cgagaccggc cgcggtgatc acggccagct ggctctgggt gggcgagaag tcgatcacgc
     5281 tggaggagtg gccggcctgc ggagggagga ccagggtcgg tacgacatcg ctctcgttgt
     5341 cgctgctgtt gtcgctgcca tcccggtggg tgaagtcgcg caggtgcgcg aacggggtgc
     5401 tgagcacgat ctcgttgggc aggtgccact gcggttcgtt gcggtggcgc atcgcacgcc
     5461 accagcgcag gttgcgggcc gcaatgtcga gtggggtggc gctgcgggtg agggcgtccg
     5521 tccagacggc gccggtcacc ctggtcatct cggtgaggct gctcatggcg gcggcggcgt
     5581 tgtacccggt gaggtgcgtc agtgcggcca tgatgtcggc cgtgcgtgcc acggcagtgc
     5641 cggtgccgga ggtcgcagtc atgtcagact cccatcgacg gtgacggtgc cgaaggtcgt
     5701 tcggtgtccg acaatcggtt actagacggt agctccgata gctatcccca acaaggtgag
     5761 cgtcagtggc gggtatgcca cgcgacagga tcgagtgggt gccctcgccc tccgcggcgt
     5821 gaagcggaat gatgactccc atgtgcgcag cgcggcccgc cgacgacgac gcgacggccg
     5881 acggctctcc cgccgccggg tggtcggccc agatcaatgc gctcggagac ttcatcaggg
     5941 cgcagcgtca gctggcgaac ctgtcgctcc gggagatggc gtcgatgacc agcgtgtcga
     6001 acgcctatct gagccaggtg gagcgcggtc tgcaccagcc gtcgctgaag gtgctgcact
     6061 cgatcgccga cgcgttgcag ctgtccaccg agcagctgct cggccaggcc ggctgggtca
     6121 ccggcacggg ggccgccacg gcgtccgcgg agtcgggcgg aaccgaggac gcgatccgca
     6181 aggacccacg gctcagtccg gagcagcgca tcgcgctgct cggcgtgtac cgcagcttcg
     6241 tcgcaggcgc cacggagtga gagggcggga tcacatcccg cttgcttgac atacttatca
     6301 aacgtgcgca gtctagatag cagacagcca accgagcacg agagagaggt caagccatga
     6361 ctgcgtccac cgaccagttc gccgacatcg ccactcgtac gcaggaggcg gtcaccaccg
     6421 ccgtgcgtac ctgggccgac accgttcaga cgttcaccgg cagcatcacg ggctcccgcc
     6481 cggccctgcc cgacgcccag gtcgtcgtcg accggtactt cgacttcgcc gagcaggtcc
     6541 tggagaacca gcgccggctc gccaagagcg tgctgaccgc aggggcccag gccgccgaga
     6601 gcgtcacgga gcaggctgcg cgcaccgccg agtcggtcgc cgcgcgcacg gtgaacgcca
     6661 ccgagaccgt tgcgaccaag gccgccgacg tcaccaaggc ggcaggcgag cgtacggcag
     6721 cctcggcccg cgccgcgcag aacgccagca agtgagctga cagcaccacc tcgaccacct
     6781 ggcgctgact gcagcacgcg agcagtcgca ggagcgcctg gcgcaggagc cccgcccggc
     6841 agaacgccgg cggggcttcc gccgtctcag acctccacga tcacggtgaa cggaccgtcg
     6901 ttcaccgact ccacggccat tcgggcgccg aacacccccg tcgccacctc cgccccgcgc
     6961 gcccgcagcg cggccaccac ggccttgacc agcggctcgg cgtgctcggg ccgggcggcc
     7021 gccgaccagg agggccgcct gcccttgcgc gtgtcgccgt agagcgtgaa ctggctgatc
     7081 accagcagcg gcgcatcctc ctcggcgcag gaccgctcct ccggcaggat ccggagctcg
     7141 tgcagcttgc gggccatcgc gggcgcggcc tcggggctgt cgtcgcgatg caccccgaga
     7201 agcacgagca ggcccagccg gtcgatctgc ccgacgacct cgccgtccac gtgcacgctg
     7261 gcccggctca cccgggcggc caccgccctc accggccctg acccagctcc ccggcgggca
     7321 ccaggacgcc gtgccgcacg aactcgcgca ccgcgggcag cgccgccgcg accaactcgc
     7381 cggtgggccg gtcgtgggcg aacgacagca gctccaccag ctccccgagc ggcaaagccc
     7441 cctgacaccc ggccagcaac gcggcggccg ggccgtccac ctcatggcgc cagcgtggcc
     7501 cgtcccgccg ggccaccgcg gagccgacct cggcccaccc gtccgcaccc gcctccgcgt
     7561 acctctcgag caccaccgac ggcgctacgg tcaggcgggc tcccagcagc gcctcgtcgc
     7621 ccgtgtgggc gcgcagccag tccacccggt ccagccagcc ctcgacctcc gggcccagcg
     7681 ggtcctcgat gtcgccgggc atgtcctcga agacaaccag cggatcaccg ccacggggtc
     7741 ggcgcagggt gacgaagccg aagccgaccc cctccacccg ttcccggtcc agccagtcca
     7801 gccacgcacc ggcctgctcg cggacggcgg gtgcggtgag gtcgagcccc gcgtcgcgct
     7861 gccaggtgcc gacgtgcaac gccgggtcgg cgacctcgcg ctgcacgatc caggcgtcga
     7921 caccggcggg cagccaggac cgcaccctgt cgggccagtc ctccccgcgc acatgcagcc
     7981 atgagccgag cagctgcgcc accccgcccg gttccaggtg ctcgggcagc gccccgagca
     8041 gcgcggcgag tgccgcgtcg ccgtcctggc ccgagtcacg gtagacgtag tccacccgag
     8101 ccgggccggg cacgaacggc gggttggaga cgatctggtc gaaccggcgg ccttccacag
     8161 gttcgagcca cgggccgtcc agcagttcga cgtcgaggtc gttgagggcg aatgtggcgc
     8221 gggccatcgc gagtgcacgc ggcgcgaggt cggtgccggt gacggccccc gcgtggcggg
     8281 tggcgtgcag ggcctgcacg ccgcaccccg tgcccaggtc gagcagtgag ccgaccgggc
     8341 ggcgcgctgt cgcggcggcc agcgtcagcg acgcggcgcc cactcccgtg acgtggtcgc
     8401 gctcctggcg gcgctggtcg aggtcggaga ggacccacca gtcgccgccc gtgtcgccgt
     8461 ccatgtggtc ggcactgtcc gaggtgtcgc cgtacgggcg caggtcgagc gccgcggtcc
     8521 agccgtcgcc ggcggcccgg agcagcccgg ccgcggcggc gtcggcgggg gcgagcgggg
     8581 cgagcgcggc ggtcacggcg gcgtgcggct ccacggaccc gagcagcagc agtcgcacga
     8641 gcacaccgag ctccccgccg tcccgggcgg cgcggaacgc gggctccacc tctccccggc
     8701 cgagcgcggc gtgcgctgcc ggcccgagca acgccaggac accgtcggtg gtgtagccgg
     8761 cccgcagcag ggcggtgcgc agcgagtcgg cggtggcggg ggtgaggtgg ggtcgcacct
     8821 gagagatcct cccagccgcc gtcgcggcgc tacgcttccg ccgtgcctct ctgcctgagt
     8881 caggacaccg atgccgacgc gcttctcgac cgcgacccgt tcgcgctgct ggtcgggatg
     8941 ctgctggacc agcagtttcc gatggagcgc gccttcgccg gaccggccct gctggcgcaa
     9001 cgcctcggcc gcaccgagct cgacgcccgc gaactggcgt cctacgaccc ggacgcgctc
     9061 gtcgagatct tccgtggccc gcccgccgtg caccgctatc cggggtcgat ggccgggcgc
     9121 gtgcaggcgc tggccgccgc cgtcgtcgac cagtacgacg gcgacgtcac cctgatctgg
     9181 cgcgacgtcg aggacggccg cgagctgttc cgcaggctca acgccctgcc cggtttcggc
     9241 acgcagaagg cgcagatctt cacggcactg ctcggcaagc agcgcggcgt cacaccggcg
     9301 ggctggcgcg aggccgcggg cggctacggc caggaggggt cgtaccgctc ggtcgccgac
     9361 gtcgtcgacg tcgcctcgct gggccgggtc cgcgagtaca agcaggcgca gaaggcggcg
     9421 gcgaaggcgg gatcgaaggc cgcggccccg gcgaagggct gaccggtcgg cccgccacta
     9481 acatcgaagt cgtgcaccag gtgcccccag cgcgtcgtga cgtccgcgtc atcgaccccg
     9541 accaggtgtg cgacgccgca gccgtgctcg ccgacggcgc ccagctgcag cactccgcgg
     9601 cggtactcga cgcgctcggc gacgtccacc ggttgagcat cctgctcgcc ctgcaccacg
     9661 ccggtgacct gtgcgtctcc gacctggcgt tcgcagtggg catgagcgac agcgcggtgt
     9721 cgcacgcgct gcggctgctg cgggcgcacg ggatggtcag cgcccagcgc gagggcaggc
     9781 tcgtccggta ccgggtggtc ggtgagctga cgacacggct gctcgacgtc gtgtccgagg
     9841 gcgtggtggg cgtcgacgtc gtgctgcacg cgcatggcgc cgccggggcg cacggggagg
     9901 cggtggcggt ggtcaacgac tgacggtcgt cggagccgcg acagtttcac tgcgcatttc
     9961 ggtgaggccc cagggtcgac aggcgcgccg acccggtgag tggcacgatg tcccaggcgg
    10021 acgaagtgcc tccggccggg ccaccgctca gcgcagcacc gccgggtgac agcgccgtcg
    10081 aggccaacga gggagttccc gtggcagacc cgcagcgtag tggggtggag ccggttctga
    10141 tcactgatgc cgctcgttcc tacgaggagg agctggccac ccgcaagcgc cgctacaaga
    10201 tcatgatggg gatgcgcatc ccgttcatgg tgctggcggc cgtcttctac cagattccgt
    10261 ggctcgcggt gacgctgctc gtgctgtcca tcccgctgcc ctggatcgcg gtcctgatcg
    10321 ccaacgaccg gctgccgatc aaggcggagg ccgtcagccg ctacgaggcc gagcggcgcc
    10381 agatcgaggc ccgcgagcac cccgtcatcg agggctgacg cctcgccggc cggtgggacg
    10441 ggctccgagc ccgcccaccg cggcggcgcc cgccgcgcag cccgcccgca gggcgtcggc
    10501 cgggccggca ccccgcagcc acgccacgag cagcccggcg tcgaaggcgt ccccggcccc
    10561 cgtcgggtcc accacgtcca cccccggggc cgtgacctcc cagcggccac ggtggtcgac
    10621 ccaccgggca ccccgggctc cctctgtgac gcagaccgcg cccgccgtgc ccatctcgac
    10681 cagcaactcc gtctccgagc cgccgagcgc cgcgagctcg tcggtgttgg gcagcagcag
    10741 gtcgataccc cgcacccact cccggaactc cggcgtgagc gcgggagcag cctgcgggtc
    10801 gacagacgtg ctcagcccgg ccgcgcgggc ggcggccagc gccgcgagcc cggccggccg
    10861 ggacgacgga tcgagcagca cgtagccaga gaggtgcagg tggtcggcgc cgtcgagcgc
    10921 gggcaggtcg gcgggatcca ggcgcgccgc cgctccccgg tccgacagca tcgtgcgctc
    10981 accctcgtcg agcagcacca cgaccgtgca ggtcggcacg tccggatcca cggtcagcgc
    11041 cgggagtaca ccggcgtccg ccaggtcggc tgcggccgca ctcccggcgg cgtcctcgcc
    11101 cacccgcgcg accagcgtga cgtcggcacc gaggtgggcg agccacgcgg ccgtgttcgc
    11161 cccggcgccc ccggcgccgg tacggatccg ggccgggacg tccgccccgt gcacggcagg
    11221 cccggacggt gcgacgagca cgtcgaccgc gacgtcgccg accaccacga cccggggacg
    11281 gcgtgtgccc ggcaccgccc caccactccc gctcacgggc gcggacgggc ggcgagtgcc
    11341 acggcgacct gcgcggcaag ctccgcgttg gcccgcacga gcgcgatgtt cgtgcgcagg
    11401 ctggccccac cgctgccggc gtggaaatgc tccagcagcg cgggcgtgac gtccttgccc
    11461 gtgatgcggc gccgtgccag cagggccgtt ccctcctcga gcagccggtc gtgcaggccg
    11521 gcgttgagct cgtcggcctc ggggacgggc ttggccagca ccatccccga cggggagccg
    11581 agcgagcggt gggctcgcca gacggcggcg gcctcggtgg gtgtgtccac ccgccagggc
    11641 accgggtgcc cggagtcgcg ccggtagaag ccgggaaagg cgtcggtgcg gtagccgatc
    11701 accggcaccg acccggtctc gagcacctcg agggtcgcgg gcacgtccag gatcgacttc
    11761 acccccgagc agacgacgaa cacccccgtg gccgccagcg cggcgaggtc ggcggagacg
    11821 tcccaggtgt cccgggcacc gcggtgaact ccgcccaggc cacccgtggc gaacagcgcc
    11881 acgcccaccg catgcgccag tgccgccgtt gcggccaccg tcgtcgcgcc gtcccggccc
    11941 agcgcgactg ccacgccgag gtcgcgtgcc gacagcttga cgagcccgcc gccgcacacc
    12001 tggtccagct cggggccgga cagcccgacg cggggcaccc cgtcgaggac ggcgacggtg
    12061 gccggcaccg cgccgtgggc ccggaccgcg gtctcgagat cgtcggcggc cgcgcggttg
    12121 tgcgggctgg gcaggccgtg ggcgagcaac gtgctctcca gcgcgacgac ggggcgtttg
    12181 gtcgcgacgg cgtcggcgac ctccggggac atcacgatcg gtgtcggcgg cggcagggcg
    12241 ggtggggaga cgggaacggg cacgtgcggc atcatggagg acatggccac ccaggtgctg
    12301 cccgacgtcg agacccgccc agagggcacc gacacgacgg gcaacgacga gcccaagatg
    12361 ttccactacg tgaagaagaa caagatcgcg gagagcgcgg tgttgggaaa catggtggtg
    12421 gcgctctgcg gcgagacctt tccggtcacc cgctcgccga agcccggctc gccggtctgc
    12481 ccggactgca agaagatcta cgactcgctg cgccctggcg gcgacgactg accgaacatc
    12541 gggtgagcac agtgccgatg cgtagcgtcc tgcgcggatg gctgtacgac cgtctggtgg
    12601 ccgggatgac caccgactgg tatcgcgagg tgctgatcag gctcccggac ggtgcgcgcc
    12661 tcctcgatgt gggcatcggc accggcgcgg cactggcccg ctgtgccgat gtggtgcgcg
    12721 ccaagcggct cgacgtcgtc ggcctcgata tcgacaccga ctacctcgaa cggtgccgcg
    12781 cagagatcgc gggcgcgcag ctgtccgcgc aggtctcgcc ggtgctgtcg tcggtctatg
    12841 accaccacgg cggcccctat gacgcggtgt acttcagcgc cagcctgatg ctgttgcccg
    12901 acccggtcgc ggccatcgtg cacgtggggg gtcagctcgc cccggacggg cggctgttcg
    12961 ccacgcagac cttccaccac cgccgctcgc ccctgctgga gcgggcgaag ccgctggtcc
    13021 gacttgtcac gaccatcgag ttcgggcgcg tgacgtacga ggacgacttc cgtcgggcgt
    13081 tcgccgccgc cggtatggag ctgatggagc tcaccaccat gagcagcacg cggcactcgt
    13141 cgtaccggct cgccgtggcc cgggtcagcg accacggcaa cggtaggagc acgagggcgg
    13201 cctgagcccg ggccggttca gcgctgcagc acgtactccc cgggtgcggg agccaccgca
    13261 tgcatcccgc gcccgccgag ctccggcggg gcgtcgcgca gcgggccgct gcgctcgtcc
    13321 agccacgcca ggtggtccga ccaccacgag ccggcgaccg ctcgggcccc ggcgagccag
    13381 cgctccgcct cagcgggtgt ggtgtccgcg acgcggaagc tcgccgtggc ctcaccgggc
    13441 gggcacacca ccgacgcggt ggagccgccg agcacgaacc ggctggtacc gccgagcaac
    13501 agggtggtgc ggtagcagga tcgccacgtg gacgggcggc cggtgatggc ggcgacgacg
    13561 tacacgtccc ggtcgacctt cgacaggtcg accgccgtgc cgagcatgcc gcacgcgccc
    13621 ggcgtgacca gcgggttgtc cagagcgatg gtcaccaggt cgcggtgcag ccgcgccggc
    13681 aggcgcacgg tgtcggcgag ccagaacatg acgtcggacg ccgggggaga ctgggcgccc
    13741 aggtagttgt gcacccagta gggccagacg aggtcggccg ggcgcagcca cgcgtgcgcc
    13801 tcggccagga cgcggccgtc gaggtagcca gtgcgctggg aggccgccat cgccgcccgt
    13861 gccttcggga tgagggttgc gcccgcggtg ccggcttcct cgtgatcgag cacggaggcc
    13921 acgaacgtca ccgacgccac ccgctcctgc gccccgatct ccgcgaggtg cgccagcagc
    13981 atcgcggtga tcgtcccccc cgaaccgaac gccatcatcg acatccggtc ggtgcgggcg
    14041 acgtgctcac acgcgtccat cgcgtcgagg actgcccgtc cgtaggtgtc gaggtcccag
    14101 gtggcgtgct cggtgccggg gttacgccag gacaggagga acacctgctg gccgccgcgg
    14161 acgaggtgct cgacgatgct gcgtcccgg
//
LOCUS       DASRSC010000300         3411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2907320,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000300 DASRSC010000000
VERSION     DASRSC010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2907320"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /locus_tag="VFE39_14875"
     CDS             <1..282
                     /locus_tag="VFE39_14875"
                     /inference="COORDINATES: protein motif:HMM:NF025210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET6259921.1"
                     /translation="EVLPDPGLAGVEWHCLQIEGPFDFDERGVLASVVDPLAHAGVSV
                     FVVATYDTDYLLVTDIDGARRALLAAGHEIRPAVTDAGTRPSATEGGHR"
     gene            279..1064
                     /locus_tag="VFE39_14880"
     CDS             279..1064
                     /locus_tag="VFE39_14880"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HET6259922.1"
                     /translation="MSTDNRWVVRRRVLQAPRLRMFCFPYAGGGASVYRDWPRWLPAD
                     IEVCAVQPPGRESRLAEPPYARLPDLLPPLADALSGLLDGPFVTFGHSLGGLVSFELV
                     KYLRRAGGPQPEQMFVSGRRPPHLPGRERPIHDLPDDDLLTAVLRLNGTSPEIRSHPE
                     LLELAIPLLRADFAVSETYHYTPEPPLECPLTAFSGLSDPYVTRSEAASWASHTRSGF
                     VLRMLPGDHFFLSTAQGQLLAEITQLLARPPSGYRADASPPAG"
     gene            1177..2382
                     /locus_tag="VFE39_14885"
     CDS             1177..2382
                     /locus_tag="VFE39_14885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6259923.1"
                     /translation="MTEAPALPLYMQREQFDPVDELVQTRDNDGVTRVPTLFGGPPAY
                     LVTRYEDVREVLSDAVRFSNARTRPFQQPDGPHFTDEELEQMRAGQMLAFDPPDHTRL
                     RRMLTPEFTVRRMRRLEPRIVEIVDAALDDIERAGKPADLVSTFAMPVPSLVICELLG
                     VPYADRAEFQERTGRLLDVSVPMRERMAVVRENRAYMARLVDGAMAAPGEDMIGMLIR
                     EHGDDLSHHELIGIASLLLVAGHETTSNMLGLGTLALLRHPDQLALVRDDPAMVGPAV
                     EELLRWLSIVHSGVPRTTTTEVEIAGHTIPAGELVVLALPAANRDPAFITDPGRLDIN
                     RGAPGHVAFGHGVHHCLGAPLARMEMRIAFPALLRRFPNLALAAPDDEVDFHTYNAIY
                     GLRSLPITW"
     gene            2430..2624
                     /locus_tag="VFE39_14890"
     CDS             2430..2624
                     /locus_tag="VFE39_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET6259924.1"
                     /translation="MKIAADRDTCIGAGMCVLTAPAFFDQDDDGIVQLLVEDVSPGDA
                     DVARQAVALCPSGALRVLDD"
     gene            2665..3267
                     /locus_tag="VFE39_14895"
     CDS             2665..3267
                     /locus_tag="VFE39_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6259925.1"
                     /translation="MTATRGQGLRADAARNYERIVVAAGQAFEEVGPAVTLEEVARRA
                     GVGVATVYRRFRNRDQLVRAVFEQLVTAEIEPATAMETDDPWRDLVGALESTVDVLAR
                     RQVVLALARETDAVDVESVRRFVHSMERPLRRAAAAGVVRPELEARDLAAVTVMALAT
                     VHPGDPGGADRRRYLALLIDGLRPAPTTLPPPLTREVGGV"
BASE COUNT          486 a         1220 c         1205 g          500 t
ORIGIN      
        1 gaggtgctgc cggatcccgg cctcgccggc gtcgagtggc actgcctgca gatcgagggg
       61 cccttcgact tcgacgaacg tggcgtgctc gcgtcggtgg tcgacccgct ggcgcacgcg
      121 ggcgtgagcg tgttcgtggt ggccacgtac gacaccgact acctactggt gactgacatc
      181 gatggggcaa ggcgggcgct gctcgccgcg ggccacgaga tccgccccgc ggttaccgat
      241 gccgggaccc ggcccagcgc aaccgagggc gggcacaggt gagcaccgac aaccgatggg
      301 tggttcggcg acgggtactg caggcgccgc ggctgcgcat gttctgcttc ccctacgccg
      361 gtggcggtgc gtccgtctat cgggactggc cacggtggtt gccggccgac atcgaggtgt
      421 gcgccgtgca gccgcccggc cgggagtccc ggctggccga accgccctac gcccgcctgc
      481 ccgacctgct gccgccgctc gcggacgcgc tgagcggact gctggacgga ccgttcgtga
      541 ccttcgggca cagccttggg gggctggtca gcttcgagct ggtcaagtac ctgcggcgtg
      601 ccggtgggcc gcagccggaa cagatgttcg tgtccggtcg acggcccccg cacctgcccg
      661 gccgggaacg gccgatccac gacctgcccg acgacgacct cctgacggcc gtcctgcggc
      721 tgaacggcac gagcccggag attcgctcgc accccgaact gctggagctg gcgatccccc
      781 tgctgcgcgc cgacttcgct gtcagcgaga cctaccacta cacacccgaa cccccgctgg
      841 agtgtccgct caccgcgttc tccgggctgt ccgacccgta cgtcaccagg tcggaggcgg
      901 ccagctgggc gagccacacg cgcagcgggt tcgtgctccg catgctgccc ggcgaccatt
      961 tcttcctgag caccgcgcag ggccagctgc tcgccgaaat cacccagctg ctcgccaggc
     1021 ccccctccgg ctacagggcg gacgcgtcac ccccggcggg ctgaccggca aggtgatcag
     1081 cacagcgctt gacgaggcgc acaatccgat agccaccttt cacttcggtg ctagcatcga
     1141 cggtgtcctt cgagcaagaa agagggtgag caggcgatga cagaagcgcc ggcgctgccg
     1201 ttgtacatgc agcgcgagca gttcgacccg gtcgacgagc tcgtgcagac cagggacaac
     1261 gatggcgtca cccgggttcc gaccctgttc ggcggtcccc ccgcgtatct ggtcacccgg
     1321 tacgaggacg tccgcgaggt actgtccgac gcggtccgct tcagcaacgc ccggacgcgg
     1381 ccgttccagc agcccgacgg tccccacttc accgacgagg agctcgagca gatgcgggcc
     1441 gggcagatgc tcgcgttcga cccgcccgat cacacccgac tgcggcggat gctcacgccc
     1501 gagttcaccg tgcgccggat gcgccggttg gagccgcgga tcgtcgagat cgtcgatgcc
     1561 gccctcgacg acatcgagcg ggccggcaag cctgccgacc tggtgtcgac cttcgccatg
     1621 ccggtcccgt cgctggtgat ctgcgagctg ctcggcgtgc cctacgccga ccgcgccgag
     1681 ttccaggagc gcaccgggcg cctgctcgac gtgtcggtgc cgatgaggga gcggatggcc
     1741 gtggtgcgcg agaaccgcgc gtacatggcg cgcctggtcg acggggcgat ggctgccccc
     1801 ggcgaggaca tgatcggcat gctgatccgg gagcacggcg acgatctgag ccatcacgag
     1861 ctgatcggca tcgcgtccct gctcctggtg gccgggcacg agaccacctc gaacatgctg
     1921 ggtctcggca ccctcgccct gctgcgccac cccgaccagc tcgcactggt gcgcgacgac
     1981 cccgcgatgg tggggccggc cgtcgaggag ctgttgcgct ggctgtccat cgtgcactcc
     2041 ggcgtaccgc gcaccaccac cacggaggtg gagatcgcgg gccacacgat ccctgccggc
     2101 gagctggtgg tgctcgcact ccccgccgcc aaccgggacc cggcgttcat caccgacccg
     2161 ggccggctgg acatcaaccg cggcgcaccc ggtcacgtcg cgttcggcca cggggtgcac
     2221 cattgcctcg gggcgccgct ggcccggatg gagatgcgca tcgcgttccc cgcgctgctg
     2281 cggcgcttcc cgaacctcgc cctggccgcg ccggacgacg aggtcgactt ccacacctac
     2341 aacgcgatct acgggctgcg ctcgctcccc atcacctggt gagcggcaca gcgacaggac
     2401 aggacagcga caggacagga ggctcgggca tgaagatcgc cgcggaccgc gatacctgca
     2461 tcggggcggg gatgtgtgtg ctcaccgcgc ccgccttctt cgaccaggac gacgacggca
     2521 tcgtccagct cctggtcgag gacgtatcgc cgggggacgc cgatgtggct cggcaggctg
     2581 tcgccctgtg cccgtccggt gcgctgcgcg tgctggacga ctgagggcca cgcgctccga
     2641 cgacatgctt gttagcgggc gcggatgacg gcgacgcgcg ggcagggctt gcgagccgac
     2701 gctgcgcgca actacgaacg aatcgtcgtc gccgcaggcc aggccttcga ggaggtcggg
     2761 cccgccgtca cgctcgagga ggtcgcgcgg cgcgccggcg tgggggtcgc cacggtctac
     2821 cggcgcttcc gcaaccgcga ccagctggtg cgcgcggtct tcgagcagct cgtcaccgcc
     2881 gagatcgagc cggccacggc gatggagacc gacgatccgt ggcgcgacct ggtcggcgcg
     2941 ctcgagtcca cggtcgacgt gctcgcccga cggcaggtgg tgctggcgct cgcccgcgag
     3001 acggacgcgg tcgacgtgga gagcgtgcgc cgcttcgtcc actcgatgga acgcccgctg
     3061 cgccgcgccg cagccgccgg cgtcgtgcgg ccggagctgg aggcccgcga cctggccgcg
     3121 gtgaccgtca tggccctggc cacggtgcat cccggtgacc cgggtggtgc cgaccgcagg
     3181 cgttacctcg ccctgctgat cgacgggttg cgaccggcgc cgaccaccct gccgcccccg
     3241 ttgacccggg aggtcggcgg cgtctgacgg agacgagagg atgtgcgggt catcgtggtc
     3301 ggggcaacga ggagggaaac ccgtgggctt ctggatctgt cggacctgcg ccgtcgagca
     3361 cgccgaactg gtcgacgtct gccggatctg tgccgacgag cggcagtggg t
//
LOCUS       DASRSC010000301         4654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1104326,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000301 DASRSC010000000
VERSION     DASRSC010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104326"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1566)
                     /locus_tag="VFE39_14900"
     CDS             complement(19..1566)
                     /locus_tag="VFE39_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HET6259926.1"
                     /translation="MSDTFDYVIVGGGSSGCALAARLSEDPDVTVCLLEAGPSDVDDP
                     AILKLTDWMALLDSGYDWDYLVEPQEHGNSFLRHARAKVLGGCSSHNSCIAFWTPRED
                     LDEWASMGCEGWSADDCWPLIERLETNDGPWTGHGRSGPVTIVQVAPDDPCGVAVLEA
                     AAAAGLPTVRFNEGSTICEGAGFFQINREPDGTRASSSRAYLHPVMDSRPNLEIRTGA
                     WASRVLFDGTRATGVEYQQDIGPGRVTVSARREVVLSAGSIDTPKLLMLSGIGPAEHL
                     RDFGIDVLVDAPGVGSNLDDHVEGLVMWEAAQPMVTRSTQWWEIGLFTRTQPGLDRPD
                     LMMHYGSVPFDLNTVRWGYPTTDNGFCLTPNVTRGRSRGTVRLRTRDFRDRPKVDPRY
                     FTDPEGHDMAVMLAGVKLARRIAEQQPLKEWVARELTPGPDAVTDDELADYITRTHNT
                     VYHPACTAKMGPATDPMAVVDPQLRVRGVDGLRVADASILPFLPAINPNITCMMIGEK
                     ASDLLRG"
     gene            complement(1563..3128)
                     /locus_tag="VFE39_14905"
     CDS             complement(1563..3128)
                     /locus_tag="VFE39_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET6259927.1"
                     /translation="MTAAGNKDSEDLAEFGYSQTLDRSIGKFASFAAGISYISILTGT
                     FQLFYFGFAFGGPAYWWSWPMVFVGQLMVALCFAELAGRYPVAGSVYNWSKRLGSATT
                     SWLAGWVMFTASVVTLTAVVLAYQITLPQIWSGFQLVGDGTGDTDFAVSAVIWGAILI
                     LFTTLVNAFGVKLMSRINSAGVFIELVAAVLLVVLLAVNIVNPPSVIVDTQGLGSDSS
                     LGYLGAFLVASLASAYVMYGFDTASSLGEETVDPRRTAPKAILRAVIASFVIGGLILL
                     FAILAAPNLGDPALGTSAGGLQLIVLQALGTNLGKVFLICIVVAVTVCALAVHTAGIR
                     LMFAMARDNALPGGARLARIDPRRKTPVVPAVLIGVVAILILVVNIGEPKIFTAVTSV
                     AVIMIYLAYLLVTAPMLRKRLRGEWPEPGGSTGYFSLGRFGLPVNILAVVWGAAMALN
                     LAWPRSEVYGEGALAWIAFIFIGAIVVIGLAWYLAKGRHQVGTLPEHMAKQLALDPAV
                     PPATESFPEGSQA"
     gene            complement(3154..4620)
                     /locus_tag="VFE39_14910"
     CDS             complement(3154..4620)
                     /locus_tag="VFE39_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6259928.1"
                     /translation="MPSLFVDGAWTSGCGGTADVINPFDASVVETVDQAGADDVDRAV
                     LAARAAFDGGPWRRIPAADRGALLRRVADLLVRDKEQIARTETLDTGKTLVESRIDVD
                     DVTAVFRYYADLAGKDAGRIVDTGQPHVLSRVVAEPVGVCVLITPWNYPLLQLSWKVA
                     PAIAAGNTMVIKPSEVTPLTSVLLVRLIEEAGAPPGVVNILLGDGRSVGAPLTEHPAV
                     DMVSFTGGLATGQAIIRASAATVKRVAVELGGKNPNIVFADTDFETAVDYALTAVFLH
                     SGQVCSAGARLIVEDSVHDDLVAEVARRAERIRLGNGLEEGSESGPLVSAAHRAKIED
                     FVASAQAEGARLVAGGRRPEEPELQKGFFYRPTVFTDVRRDMRVIREETFGPILTVER
                     FSTEEEAIALGNDTEYGLAGAVWTSDMGRAERVAAALRHGTVWINDYHPYVPAAEWGG
                     FKRSGNGRELGPTGLAEYQELKHVWHNTAPAPMRWFTG"
BASE COUNT          728 a         1662 c         1590 g          674 t
ORIGIN      
        1 tatccgcgca cgggggcgtc agccccgtag caggtccgac gccttctccc cgatcatcat
       61 gcaggtgatg ttggggttga tagccgggag aaacggcagg atcgacgcgt ccgcgacccg
      121 cagcccgtcg accccgcgca cccgcagctg cggatccacc accgccatcg ggtcggtcgc
      181 tgggcccatc ttcgcggtgc acgccgggtg gtagacggtg ttgtgcgtcc tggtgatgta
      241 gtcggccagc tcgtcgtcgg tcaccgcgtc cgggccgggt gtgagctcgc gcgccaccca
      301 ctccttgagc ggctgctgct cggcgatccg ccgcgcgagc ttcaccccgg cgagcatcac
      361 ggccatgtcg tggccctcgg gatcggtgaa gtagcgcggg tcgaccttgg gccggtcccg
      421 gaagtcgcgg gtgcgcagcc gcacggtgcc gcgtgaccgg ccgcgcgtca cgttcggcgt
      481 gaggcagaag ccgttgtccg tggtggggta accccaccgc acggtgttca ggtcgaacgg
      541 caccgagccg tagtgcatca tcaggtccgg gcggtccagg cccggctgtg tgcgcgtgaa
      601 cagcccgatc tcccaccatt gcgtggagcg ggtgaccatc ggctgcgccg cctcccacat
      661 cacgaggccc tcgacgtggt cgtccaggtt ggacccgacg ccaggggcgt ccacgagcac
      721 gtcgatcccg aagtcgcgca ggtgctcggc gggcccgatc ccggagagca tcagcagctt
      781 gggtgtgtcg atcgacccgg cggagagcac cacctcgcgg cgagccgaaa cggtgacgcg
      841 gccgggtccg atgtcctgct ggtactcgac gcccgtcgcg cgcgtcccgt cgaagaggac
      901 acgcgacgcc cacgcccccg tgcggatctc caggttcggc cgcgagtcca taacggggtg
      961 caggtacgcg cgcgacgacg aggcgcgcgt gccgtcgggc tcgcggttga tctggaagaa
     1021 gccagccccc tcgcagatcg tgctgccctc gttgaaccgc acggtgggca gccctgcggc
     1081 ggccgctgcc tcgagcaccg cgacaccgca ggggtcgtcg ggcgccacct gcacgatggt
     1141 cacggggccg gagcggccgt gccccgtcca cggcccgtcg ttggtctcca accgctcgat
     1201 gagcggccag cagtcgtcgg cgctccagcc ctcgcagccc atcgacgccc actcgtcgag
     1261 gtcctcgcgg ggcgtccaga acgcgatgca cgagttgtgc gacgagcacc cgccgagcac
     1321 cttcgcccgc gcatgacgca ggaagctgtt gccgtgttcc tgcggctcga cgaggtagtc
     1381 ccagtcgtag ccggagtcga gcagcgccat ccagtcggtg agcttgagga tcgccgggtc
     1441 gtccacgtcg gacggtccgg cctcgagcag gcacaccgtg acgtcgggat cctccgacag
     1501 ccgcgccgcc agcgcgcagc ccgacgatcc cccgccgacg atcacatagt cgaacgtgtc
     1561 gctcacgcct ggctcccctc cgggaacgac tccgtggccg gagggaccgc ggggtcgagg
     1621 gcgagctgct tcgccatgtg ctcggggagc gtgccgacct ggtggcgtcc cttcgcgagg
     1681 taccaggcca ggccgatcac cacgatggcg ccgatgaaga tgaacgcgat ccaggcgagc
     1741 gccccctccc cgtagacctc cgagcggggc cacgcgaggt tgagtgccat cgcggcgccc
     1801 cacacgacgg cgaggatgtt caccggcagg ccgaaccgcc ccagcgagaa gtatccggtc
     1861 gacccgccgg gctccggcca ttcgccgcgc agccgcttgc gcagcatcgg cgcggtgacc
     1921 aggaggtagg ccaggtagat catgatgacg gccacgctgg tgacggccgt gaagatcttc
     1981 ggctcaccga tgttcaccac gaggatcagg atggcgacga cgccgatcag cacggccggc
     2041 accacggggg tcttgcgccg tgggtcgatc cgcgccaacc gggcgccgcc cggcagggcg
     2101 ttgtcgcggg ccatggcgaa catcaaccgg atgcccgcgg tgtgcacggc cagtgcgcag
     2161 accgtgacgg cgacgacgat gcagatcagg aacaccttgc cgaggttcgt gccgagcgcc
     2221 tgcagcacga tcagctgcag cccgcccgcg ctggtaccca gagccggatc gcccaggttc
     2281 ggcgcggcga ggatcgcgaa cagcaggatc agcccaccga tgacgaacga cgcgatgacc
     2341 gcgcgcagga tcgccttcgg cgcggtgcgc cgcgggtcga cggtctcctc ccccagggag
     2401 ctggcggtgt cgaagccgta catgacgtac gcgctggcca gcgacgcgac gaggaacgcc
     2461 ccgaggtagc cgagcgagct gtcggagccc agcccctggg tgtcgacgat gacgctcggc
     2521 gggttcacga tgttcacggc cagcagcacg accaacagga ccgccgcgac gagctcgatg
     2581 aagacaccgg cgctgttgat ccgcgacatc agcttcacgc cgaacgcgtt gaccagcgtc
     2641 gtgaagagaa tgaggatcgc gccccagatc accgcgctca cggcgaagtc ggtgtcgccg
     2701 gtgccgtcac cgaccagctg gaagccgctc cagatctgcg gcaacgtgat ctggtacgcc
     2761 agcaccaccg cggtgagcgt caccaccgat gccgtgaaca tcacccagcc ggccagccac
     2821 gacgtcgtgg cgctgcccag ccgcttcgac cagttgtaga ccgacccggc caccgggtag
     2881 cgcccggcca gctcggcgaa acacagcgcc accatcagct gcccgacgaa caccatgggc
     2941 caggaccacc agtaggcggg gccaccgaag gcgaaaccga agtagaagag ctggaacgtg
     3001 ccggtcagga tcgagatgta gctgatcccg gcagcgaagc tggcgaattt gccgatgctg
     3061 cggtccagcg tctggctgta cccgaactcg gcgagatcct cgctgtcctt gttgcccgct
     3121 gctgtcatcg tgtcgcctcc gaggatcgat ccatcagccc gtgaaccacc gcatcggcgc
     3181 aggtgccgtg ttgtgccaga cgtgcttgag ctcctggtac tcggccagcc ccgtcgggcc
     3241 gagctcccgt ccgttgcccg agcgcttgaa gccgccccac tccgcggccg ggacgtaggg
     3301 gtggtaatcg ttgatccaca ccgtgccgtg tcgcagcgcg gccgccaccc gctcggcgcg
     3361 gcccatgtcg gacgtccaca ccgcacccgc gaggccgtat tcggtgtcgt tgcccagcgc
     3421 gatggcctcc tcctcggtgg agaagcgctc cacggtgagg atcgggccga acgtctcctc
     3481 gcggatcacg cgcatgtcgc ggcgcacgtc ggtgaagacg gtgggccggt agaagaagcc
     3541 cttctgcagc tcgggctcct caggacggcg cccgcccgcg accagccggg caccctcggc
     3601 ctgcgccgac gccacgaagt cctcgatctt ggcgcggtgt gccgcggaga ccagcgggcc
     3661 cgactcgctc ccctcttcca ggccgttgcc cagccggatc cgctcggcgc gccgcgcgac
     3721 ctcggccacg aggtcgtcgt gcacggagtc ctcgacgatc agccgggcgc ctgccgagca
     3781 gacctgcccc gagtgcagga acacggcggt gagcgcgtag tcgaccgcgg tctcgaagtc
     3841 ggtgtcggcg aagacgatgt tcgggttctt gccgccgagc tcgacggcca cccgcttcac
     3901 ggtggccgcg gacgcgcgga tgatcgcctg gccggtggcg aggccgccgg tgaagctcac
     3961 catgtcgacg gccgggtgct ccgtgagtgg ggcaccgaca ctgcggccgt cgccgagcag
     4021 gatgttgacc acgcccggcg gagcacccgc ctcctcgatc agccgcacca gcagcacgct
     4081 cgtcagcggg gtcacctcgc tcggcttgat caccatcgtg ttcccggccg cgatcgccgg
     4141 agcgaccttc cacgagagct gcagcagcgg gtagttccac ggcgtgatga gcacgcagac
     4201 gcccaccggc tcggcgacga cccggctcag cacgtgcggc tgccccgtgt cgacgatgcg
     4261 gccggcgtcc ttgcccgcga ggtcggcgta gtagcggaaa acggccgtca cgtcgtcgac
     4321 gtcgatgcgg ctctcgacga gcgtcttgcc ggtgtcgagc gtctcggtgc gggcgatctg
     4381 ctccttgtcc cgcacgagca ggtcggccac gcgacgcagc agcgccccgc ggtcggctgc
     4441 tgggatgcgc cgccacggcc ccccgtcgaa cgccgccctc gccgcgagca ccgcccggtc
     4501 gacgtcgtcg gcaccggcct ggtcgacggt ttcgaccacg ctcgcgtcga acggattgat
     4561 cacgtcggcg gttccaccgc acccggaagt ccatgctccg tcaacaaaga ggctgggcac
     4621 gcgttgatcg tcctcacatc aatgtcgccg tcaa
//
LOCUS       DASRSC010000302         6231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1598375,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000302 DASRSC010000000
VERSION     DASRSC010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598375"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VFE39_14915"
     CDS             <1..291
                     /locus_tag="VFE39_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259929.1"
                     /translation="RTTVTDRQGQADSSPPKRVISGLLDRDVLLWNTGFSPAVRPEVE
                     AAPLYSQLGVVRAGRSLFVEDPMVSAPWTWGTVLSLPSVVDALVKQLAGVVA"
     gene            398..2983
                     /locus_tag="VFE39_14920"
     CDS             398..2983
                     /locus_tag="VFE39_14920"
                     /EC_number="2.5.1.147"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010229368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional FO biosynthesis protein CofGH"
                     /protein_id="HET6259930.1"
                     /translation="MPEFLSVPEPPASPSALRRALRRVRDGVALDVTEASVLLAARGE
                     ALDELLSAASRVRDAGLVDAGRPGVVTYSRNVFIPLTRLCRDRCHYCTFATVPHRLPA
                     AFLERDEVLEIARAGAAAGCKEALFTLGDRPEDRWPAAREWLDARGYDSTLDYVRASA
                     IAVLEETGLLPHLNPGVLSWAELTRLKPVAVSMGMMLETTATRLWSEPGGPHYGSPDK
                     EPAVRLRAITDAGRVGVPYTTGILIGIGENRTERAESIFAIRSAARAHGHIQEVIIQN
                     FRAKPDTAMANDPDADLDDLAATIAVSRIVLGPKMRVQAPPNLVGEEFALMLRAGIDD
                     WGGVSPVTADHVSPERPWPAIDELATQTAAAGFTMRERLTAYPKYVLAGSPWIDTRLH
                     AHVAALADPETGLANASAVVTGRPWQEPDGGFESTGRIDLNTSIDAEGRTEDRRGDFD
                     SVYGDWDELRSELAAHQQAAPERLDSDVRAGLALAASDPAALLDPAHEAAAMAVLTAD
                     GAALEELTRLADALRADVVGEEVSYVVNRNINFSNVCYVGCRFCAFAQRERDADAFRL
                     SLDEVAARAVEAAQDGATEVCMQGGIDPQLPVSFYADLVRAVKAAVPDMHVHAFSPME
                     IVSASAKAGVSIEEWLTELRDAGLGSIPGTAAEILDDEVRWVLTKGKLPAAQWLEVVG
                     TAHRLGIPSSSTMMYGHVDEPRHWLGHLRTLAALQDETGGFTEFVPLPFVHHNAPIYL
                     AGLARPGPTTRDNLAVHAFARLALHGRIDHIQCSWVKLGDDLSARILQGGADDMGGTL
                     MEETISRMAGSENGSARTVTELAAIAATAGRPVRQRSTTYREPASRLVPAERTGLRLL
                     PLTSV"
     gene            3042..4049
                     /locus_tag="VFE39_14925"
     CDS             3042..4049
                     /locus_tag="VFE39_14925"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET6259931.1"
                     /translation="MPSPACPPDGVVLDVYATGVCRSDWHAWRGHDPVAVPHVPGHEF
                     AGVIAQAGPGVRSWRVGERVTAPFVCGCGGCDVCVAGDPQVCPNQTQPGFTHHGSFAE
                     QVVVHAAEHNLVRLPERLSLLAAAALGCRFSTAYRALTVHGGLAPGQWLAVHGCGGVG
                     LSAVLIASVIGARVVATDVSPSALAVARERGATATLDVSELPGPAEIAARVHEITDGG
                     AHVSVDALGSVSAATASVLSLRRRGRHVQIGLLLGEAASAPLPMDRVLSWELSIFGSH
                     GIAAHEYAAMLDLIERTGLDPASLVGQVITLEDAGTALAAMDGRTAAGMTVVQVRREI
                     R"
     gene            4046..5191
                     /locus_tag="VFE39_14930"
     CDS             4046..5191
                     /locus_tag="VFE39_14930"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6259932.1"
                     /translation="MISGRSGAEPVYVVGGGPGGLAVAAVLGRYGIPVTVLERGDAVG
                     ASWRTHYDRLHLHTVRGLSGLPGFPIPRAYGRWVARDDMVRYLEQYSAHHRLDVRTGV
                     EVERIDWANGRARWVLRTNRGDEISANSVVVATGNNHTPELPDGAGTFTGALLHAKDY
                     RNPGPYADRDVLVVGVGNTGAEIAADLAAGGAGRVRLAVRTPPHIVRRDVAGWPSQLT
                     GILMRHLPATVVDRAANWQARLSMPDLTPYGMPRPQDGLYTRILTHRVVPVQDVGIVD
                     AVRTGKVEPVASVIGFDGAVVRLADGSEITPDVVIAATGYSRGLNPIVGHLGVLDGRG
                     IPRVSGGAVAPGLYFTGYRISISGALRDMAVDARRIGRAIGRPAAAP"
     gene            5233..5400
                     /locus_tag="VFE39_14935"
     CDS             5233..5400
                     /locus_tag="VFE39_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259933.1"
                     /translation="MGILGELFPGRKISDEAGEAGEGEQWQLGPIDLDNRVVQVQRVE
                     EPEEEAPDAPS"
     gene            complement(5413..>6231)
                     /locus_tag="VFE39_14940"
     CDS             complement(5413..>6231)
                     /locus_tag="VFE39_14940"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6259934.1"
                     /translation="GARSWPGGAARPVLLVHGLSSNDRLWDGVAGRLAAAGHPVVAVD
                     LRGHGTSVDVSDTDDEDDLDAPATDATRAAARDLAAVCAGLGWTAPVVAGQAWGGNVV
                     LQLAADRADLVHALVLVDGGWLHLGDQWPTLDAAWGVLAPPQFDGMSAEVLRSSLRAG
                     HPGWTDAAVDAVLGNLRVRTDGTVTPWLSRERHRAIIGSALRHQPRTLYPLVGAPALL
                     LAATSDTTPPRKREQLAEAVAALPHARLVEFRGGDHDLHAQQPGRVAELIAGLA"
BASE COUNT          842 a         2212 c         2273 g          904 t
ORIGIN      
        1 agaacgacgg tgacggatag acaagggcag gctgactcca gtccacccaa gcgtgtgatc
       61 tctgggctgc tcgaccgcga tgtgctcctg tggaacacgg gcttcagccc ggcggtccgc
      121 ccggaggtgg aggccgcgcc gctctactcc cagctgggcg tggttcgcgc cgggcggtcc
      181 ttgttcgtcg aggacccgat ggtgtctgcg ccgtggacgt ggggcaccgt gctgagcctt
      241 ccgagcgtgg tcgacgcgct ggtcaagcag ctcgccgggg tagtggcctg agggcgcgtg
      301 cacttcgccg cggccccgct cgagcgagtg gcggccgtca cttcgcctgc tcgcgccgcc
      361 ggtcgaccgg ctcactttct cgacctagtg ttcaggagtg cctgaattcc tgagcgtgcc
      421 cgagccgccg gcgtccccct ccgccctgcg ccgtgccctg cgccgggtcc gtgacggtgt
      481 cgctctcgac gtcacggagg cgtccgtgct gctggccgcc cgcggcgagg cgctcgacga
      541 gctgctgtcg gcggcgtccc gggtgcgcga cgccgggctg gtcgatgcgg gcaggcccgg
      601 ggtcgtcacg tactcgcgca acgtgttcat cccgctcacc cgcctgtgcc gtgaccgctg
      661 ccactactgc acgttcgcca cggtgccgca ccggctgccc gccgcgttcc tcgagcgcga
      721 cgaggtgctg gagatcgcca gagcgggcgc ggcggcgggc tgcaaggaag cgctgttcac
      781 cctcggcgac cggccggagg accgctggcc cgccgcccgt gaatggctgg acgcccgcgg
      841 ctacgactcc acgctcgact acgtccgcgc cagcgcgatc gcggtgctcg aggagaccgg
      901 cctgctgccg cacctcaacc ccggtgtgct cagctgggcc gagctgacgc ggctcaagcc
      961 ggtggccgtg agcatgggga tgatgctgga gaccaccgct accaggctgt ggagcgagcc
     1021 tgggggcccg cactacggga gcccggacaa ggagccggcg gtccggctcc gtgcgatcac
     1081 cgatgccggc cgggtgggtg tcccgtacac caccgggatc ctgatcggga tcggcgagaa
     1141 tcgcaccgag cgggcggagt cgatcttcgc gatccgctcg gcggccaggg cgcacggtca
     1201 catccaggaa gtgattatcc agaacttccg cgcgaagccc gacactgcga tggcgaacga
     1261 tccagacgcc gacctcgatg atctggcggc cacgatcgcc gtctcccgga tcgtgctcgg
     1321 ccccaagatg cgggtacagg cgccaccgaa cctcgtcggt gaggagttcg cgctgatgct
     1381 gcgcgcgggc atcgatgact ggggcggcgt ctcgcccgtc accgccgacc acgtcagccc
     1441 tgagcggccg tggcccgcga tcgacgagct ggccacccag acggcagcgg ccggattcac
     1501 gatgcgcgag cgactcaccg cgtacccgaa gtacgtcctg gcgggctcgc cgtggatcga
     1561 cacgcggctg cacgcccatg tcgcggcgct cgccgacccg gagaccggcc tcgcgaacgc
     1621 gagcgccgtg gtcacggggc ggccgtggca ggaacccgac ggagggttcg agtccaccgg
     1681 ccggatcgac ctgaacacct cgatcgacgc cgaggggcgc acggaggacc ggcgcggcga
     1741 cttcgactcg gtgtacggcg actgggacga actgcgttcc gagctcgcag cgcatcagca
     1801 ggcggccccg gagcggctgg actccgacgt acgtgcgggg ctcgcgcttg ctgcctccga
     1861 tccggccgca ctgctcgacc cggcccacga ggcggcggcg atggccgtgc tcaccgccga
     1921 cggcgccgcg ctcgaggagc tcactcggct ggccgacgcg ctgcgtgccg acgtcgtggg
     1981 cgaggaggtg tcctacgtcg tcaaccgcaa catcaacttc tcgaacgtct gctacgtcgg
     2041 gtgccggttc tgcgccttcg cgcagcggga gcgtgacgcc gacgcgttcc ggctctcgct
     2101 cgacgaggtg gcagcgcggg cggtggaggc cgcgcaggac ggcgccaccg aggtgtgcat
     2161 gcagggcgga atcgacccgc agctgcctgt gtccttctac gccgacctgg tgcgggccgt
     2221 gaaggccgcg gtgcccgaca tgcacgtgca cgcgttcagc ccgatggaga tcgtcagcgc
     2281 gtcggccaag gccggcgtct cgatcgagga gtggctcacc gagctgcgcg atgccgggct
     2341 gggctcgatc ccgggcacag cggcggagat cctcgacgac gaggtccgct gggtactcac
     2401 caagggcaag ctgccggcgg cgcagtggct cgaggtcgtc ggcacggcgc accggctcgg
     2461 gatcccgtcg tcgtcgacca tgatgtacgg ccacgtcgac gagccgaggc actggctggg
     2521 ccacctccgc accctcgcgg ccctgcagga cgagacgggc gggttcaccg agttcgtgcc
     2581 gctcccgttc gtgcaccaca acgcgccgat ctacctcgca ggcctcgccc ggccgggccc
     2641 caccacgcgg gacaacctgg cggtgcacgc tttcgcccgg ctcgccctgc acggccggat
     2701 cgaccacatc cagtgctcgt gggtgaagct cggcgacgac ctctccgcgc ggatcctgca
     2761 gggcggcgcg gacgacatgg gcggcacgct catggaggag acgatcagcc ggatggcggg
     2821 ctcggagaac ggctcggcgc gcacggtgac cgagctcgcg gcgatcgccg cgactgccgg
     2881 acgccctgtg cggcagcgca gcacgacgta ccgcgagccc gcgagccgct tggtgccggc
     2941 ggagcggacg gggctccggc tgcttccact gacctccgtc tgaccggggc gtgaaggctc
     3001 tcgtcttcga ggagtacgga ggcccgcttc gggtgcgtga cgtgccgtca cctgcctgcc
     3061 cccctgacgg ggtcgtactc gacgtgtacg cgaccggggt ctgcagatcc gactggcatg
     3121 cttggcgcgg ccatgatccc gtcgcggtgc cccacgtgcc ggggcacgag ttcgccgggg
     3181 tcatcgctca ggcgggccct ggcgtccggt catggcgcgt cggcgagcgc gtgacggctc
     3241 cgttcgtgtg tggctgcggt ggatgcgacg tgtgcgtggc tggcgacccc caggtctgtc
     3301 cgaaccagac ccaacccggg ttcacccacc acggctcctt cgccgagcag gtggtcgtgc
     3361 acgcggccga gcacaacctg gtgcggcttc cggagcggct gagcctcctg gcggcagccg
     3421 cgttggggtg ccgcttctcg accgcgtacc gcgcgttgac cgtgcacggc gggctggccc
     3481 ccggccagtg gctggccgtg cacggctgcg gaggggtcgg cctctccgcc gtgctgatcg
     3541 cgtcggtgat cggagcacgg gtcgtggcga cggacgtctc gccctcggcg ctggcggtgg
     3601 cccgcgaacg aggtgcgacg gcgacgctgg atgtgtcgga gctccccggc ccggctgaga
     3661 tcgcggctcg cgtccacgag atcaccgacg gcggtgcgca cgtgtccgtc gatgccctgg
     3721 ggtccgtgtc ggccgcgacc gcctccgtgc tctcgctacg ccgccgcggg aggcacgtgc
     3781 agatcggtct cctgctgggt gaggccgcgt cagcgccgct gccgatggac cgcgtgctct
     3841 cctgggagct ttcgatcttc ggctcgcacg gcatcgcagc ccacgagtat gcggcgatgc
     3901 tcgacctcat cgagcgcacc gggctcgacc cggcgtcgct cgtcggacag gtgatcacgc
     3961 tggaggatgc cggtacggcg ctggcggcga tggacggacg cacggcggca ggcatgacgg
     4021 tggttcaggt caggcgtgag atccggtgat ctccggccgg tcgggagccg aacccgttta
     4081 cgtcgtcggc ggtgggcccg gcgggcttgc cgtggccgcg gtcctgggca ggtacgggat
     4141 tcccgtcacc gtcctcgagc gcggtgacgc cgtgggagcg agttggcgca cgcactacga
     4201 ccggttgcat ctgcacaccg tccgcggtct gtcgggcctg ccgggttttc cgatcccccg
     4261 ggcgtacggc cgttgggttg cccgcgacga catggtgcgg tacctggagc agtacagcgc
     4321 tcaccatcgt ctcgacgtcc gcaccggcgt cgaggtcgaa cggatcgact gggcgaacgg
     4381 ccgtgcccgt tgggtattgc gcaccaaccg aggcgacgag atatccgcga actcggtcgt
     4441 cgtggccacc ggcaacaacc acaccccgga gctgccggac ggggcgggaa cgttcaccgg
     4501 tgcgctgctc cacgccaagg actaccgcaa ccccggcccc tacgccgacc gcgatgtact
     4561 cgtcgtgggc gtcggcaaca ccggcgccga gatcgcggcc gacctggctg caggcggcgc
     4621 cggtcgcgta cgtctcgccg tccggacccc gccgcacatc gtccgccgtg acgtcgcggg
     4681 ctggccctcc cagctcaccg ggatcctgat gcgccatctg ccggccaccg tcgtcgaccg
     4741 ggcggccaac tggcaggccc ggttgagcat gccggacctg acgccgtacg gcatgccccg
     4801 gccacaggac ggcttgtaca cccggatcct cacccaccgg gtggtgccag tgcaggatgt
     4861 cggaatcgtc gacgcggtgc ggacgggcaa ggtcgaaccc gtggcgtcgg tgatcgggtt
     4921 cgacggggcc gtggtgcgcc tggcagacgg ttccgagatc accccggacg tcgtcatcgc
     4981 cgcgaccggc tacagccggg gcttgaaccc gatcgtcggc cacctgggcg tcctcgacgg
     5041 gcgcggcatc ccgcgggtat ccggtggcgc ggtggcacca ggcctctatt tcaccggcta
     5101 ccggatctcg atcagtggag cactccgcga catggccgtc gacgcccgac ggatcggccg
     5161 ggcgatcggc cggcctgccg cggccccatg atggcgggca cggcctcgga aggaacgtcg
     5221 tacggtgggg tcatgggcat tctgggcgag ctgtttcccg gacggaagat cagcgacgaa
     5281 gcaggcgagg cgggcgaggg cgagcagtgg cagctcggcc cgatcgatct cgacaaccgc
     5341 gtggtgcagg tgcagcgcgt cgaggagccc gaggaggagg ctccggacgc cccgagctga
     5401 tccggcgacc gctcaagcca gcccggcaat gagctccgcc acccggcccg gctgctgcgc
     5461 gtgcaggtcg tggtcaccgc cgcggaactc gaccagccgg gcgtgcggca gtgcggcgac
     5521 ggcctcggcg agctgctcgc gcttgcgcgg cggggtggtg tcgctggtcg ctgcgagcaa
     5581 caaggccggg gctccgacca gcggatacag tgtccggggc tggtgccgga gcgcgctccc
     5641 gatgatcgcc cggtgacgct cgcggctcaa ccacggggtg acggtgccgt cggtgcgcac
     5701 ccggaggttg cccaacaccg cgtcgactgc ggcatcggtc cagcccggat gcccggctcg
     5761 caggctcgat cgcagcacct cggcgctcat cccgtcgaac tggggtggcg cgagcactcc
     5821 ccaggccgcg tcgagtgtcg gccactggtc accgaggtgc agccacccgc cgtcgaccag
     5881 tacgagagcg tgcaccaggt cggcacggtc ggcggcgagc tggagcacga cgttgccgcc
     5941 ccacgcctgc ccggcgacca ccggagccgt ccagccgagg cccgcacaga ccgccgccag
     6001 atcccgggcg gcggcccgtg tcgcgtccgt tgccggggcg tcgaggtcgt cctcgtcgtc
     6061 ggtgtcggag acgtccaccg acgtcccgtg cccgcgcagg tccaccgcca ccaccggatg
     6121 cccggcggcg gcgagccgcc ccgcgacgcc gtcccacagc cggtcgttgg acgacagacc
     6181 gtgcacgagc agcaccggtc gtgcagcacc gcccggccag gacctggcgc c
//
LOCUS       DASRSC010000303         8997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2742338,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000303 DASRSC010000000
VERSION     DASRSC010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8997
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2742338"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="VFE39_14945"
     CDS             <1..754
                     /locus_tag="VFE39_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HET6259935.1"
                     /translation="LLRHPEFATRDLSSVRGISSGAAPMPVEMIEALHRRFGDDVVIV
                     EGYGLTEVTMGATLGPAWRSGVRKVGTVGLPVYDTEVRIVAPEGGNLTPLPQGEAGEV
                     CIRGPQVMVGYKDRPEETAATLVDGWLRTGDIGAIDEDGYLSIVDRKKDMLLYKGYNV
                     YPRELEELLFAQGAVTGAAVVGRPDPVVGELPVAFVVAPGLGDADAGALMEAVNEQVL
                     PYKRLRELHLVDEIPVSAAGKVLKRELRERLP"
     gene            910..1206
                     /locus_tag="VFE39_14950"
     CDS             910..1206
                     /locus_tag="VFE39_14950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259936.1"
                     /translation="MSLLPKPVVAMPTAAASPGDGVAAARSRRDPVARAGRHRRATTP
                     RTLLGLAVDVVGRVGSLVTLAALVVLAATAGAMADGMPAGGSATVTATFDRSGP"
     gene            complement(1217..1432)
                     /locus_tag="VFE39_14955"
     CDS             complement(1217..1432)
                     /locus_tag="VFE39_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259937.1"
                     /translation="MIPVPMDHHEKMRLRAAAFRATRLYPGPVGELLSRELLTWEEFG
                     YRLGGEQLVMRLVDHVLQSPIAQAEAA"
     gene            1647..2333
                     /gene="tenA"
                     /locus_tag="VFE39_14960"
     CDS             1647..2333
                     /gene="tenA"
                     /locus_tag="VFE39_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET6259938.1"
                     /translation="MVTPADSALRTRDVLWSDIEDVYAAILAHPFISGLTDGTLPRES
                     FRHYIVQDAHYLRGYAKALAVCAAKAPDEDGTVMFAAHAAGAIAAERDLHAALMGEMG
                     CTSEEAALEPIAPTTQAYVSYMLASALGGSFAEAVGAVLPCYWIYARVGEELMARSSP
                     DPLYARWIAMYGSEDFQSVVDAVLALTDRIGAGMSEGERTLMRRHFRTTSRYEWMFWD
                     AGYRCETWPV"
     gene            complement(2352..2717)
                     /locus_tag="VFE39_14965"
     CDS             complement(2352..2717)
                     /locus_tag="VFE39_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HET6259939.1"
                     /translation="MAVVAVSLWVATLIVPGIDDTAGTTSTRIGTLVVVALIFGLVNA
                     VLKPLIKVVGCPFYVLTLGLIGLVVNALLFLLVGYIASGIGLPFTVQSFGAAFVGAIV
                     VAVVGFILHFLIPDRFDQR"
     gene            complement(2753..3985)
                     /locus_tag="VFE39_14970"
     CDS             complement(2753..3985)
                     /locus_tag="VFE39_14970"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HET6259940.1"
                     /translation="MMQLKVDADPGEVGMDAGRLERIDTHFRRYVDDGRLAGWTIAVS
                     RRGRVAHLSHYGLADLEERRPVADDTIWRIYSMSKPITSVAALMLYERGALELTDPVS
                     RYIPAFADMRVYQGGSAASPGTVPATEPVRIWHLLTHTAGLTYGWHHAHAVDEIYRAK
                     GFEFGSPRGLDLAGACEVWAQVPLLFQPGTEWNYSVATDVLGRVVEVASGQSLDAFFA
                     EHVFRPLGMNDTGFSVPEDDVERLAALYGPNPAGGLLRNRSMGDAATRAPAFLSGGGG
                     LVSTAGDYHRFTQMLARGGELDGARLLGPRTVAYMTRNHLPGGADLEQFGRPIFSESP
                     YRGVGFGLGFSVVIDAAANKALTHEGEFAWGGLASTAFWIDPVAEITALFFTQLIPSS
                     TYPVRTQLRTLVNQALLD"
     gene            complement(3996..4472)
                     /locus_tag="VFE39_14975"
     CDS             complement(3996..4472)
                     /locus_tag="VFE39_14975"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET6259941.1"
                     /translation="MTNGLSGRVRGTWAVRVEAATLAGMAGGQLAVVEDWHAAVNGGD
                     AARVGDLCTSDVEVGGPRGSGFGRELILDWVGRAGIRMEPVRWFCGAGGAVVEQDARW
                     LDTHTGELGEPVRLATAFVVENGRISRVLRHPDAAGALAALGLRPVDEVGREINRM"
     gene            4560..5537
                     /locus_tag="VFE39_14980"
     CDS             4560..5537
                     /locus_tag="VFE39_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HET6259942.1"
                     /translation="MALHPEPILDISPALRGDAEVLRALLRRRDAGIRADGNRIALVV
                     SGGGMRGAYAGGMAHALDDAGLAGCFDVIYGSSAGAYIGAALLLGNGRGSAHIFFEDM
                     ACRAFIDPRRIGTRRPVVSLDHLIDHVLVHSKPMAWDRLGPSPVPLRVVATAADDLRP
                     HVLDPATPDEWKVALRATASIPLLAGPPVELHGRRWIDGSVAEPVSVLRALQDGATHV
                     LALLNRTAPELRRADLAAGPAPWARALDRLAPGLGMIAQESNRHGPALAVLDDAAHPS
                     RNGAHLLALMPEQDLGVRGLTIDVPRVTQAAAAGYATVTSALCRVRSSQ"
     gene            5649..7349
                     /locus_tag="VFE39_14985"
     CDS             5649..7349
                     /locus_tag="VFE39_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HET6259943.1"
                     /translation="MDADVIVVGAGLTGLVAAAELADAGRRVLLLDQEPETGLGGQAF
                     WAFGGLFLVDSPEQRRLGVHDSFDLAMQDWLGSAAFDRGVDDPTGEDFWGRQWATAYL
                     DFAAGEKRSWLHRQGVRWFPVVGWAERGGYLADGHGNSVPRFHITWGTGPGLLAPFER
                     RVREAAAKGLVELRFRHRVDGLTVTDGVVDGVHGAVLEPSSAARGTASSRNEVGEFEL
                     HAQAVVVTSGGIGADHEMVRANWPSRLGRPPRYMISGVPEHVDGRMLAITERAGGRIV
                     NRDRMWHYTEGVHNWGPIWARHGIRILAGPSSLWFDARGRRFPTPNFPGFDTLGTLEA
                     ITRTGYDHSWLVLTQKVIEKEFALSGSEQNPDLTGKDVRATLGRIRPGAPAPVLAFMN
                     KGVDFVVADTLAELVAGMNKLTRHNTGGADGDIDEPLIDLAELKRQIVARDREIDNPF
                     TKDLQLAAVHSARRYRGDRLARIATPHKILDPANGPLIAVRLNILTRKTLGGLQTDLS
                     CRVLRADGTPFPGLYAAGEVAGFGGGGVHGYRSLEGTFLGGCLFTGRQAGRAAGLSVS
                     "
     gene            complement(7399..7658)
                     /locus_tag="VFE39_14990"
                     /pseudo
     CDS             complement(7399..7658)
                     /locus_tag="VFE39_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004562354.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
     gene            7959..8396
                     /locus_tag="VFE39_14995"
     CDS             7959..8396
                     /locus_tag="VFE39_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259944.1"
                     /translation="MTDRGARRDARPSRAAPVTAVVLGTGDGLLLIVLATAAAGNLDR
                     WSVTDAVSFAVAVFSGLNLLWGAATLLRKMPIGRLLLTTTAGTIGVGAVVLLLVQPAP
                     SGRLVDVVTGVLLVGLQTVIISCAMSRPTLLWIVGGPGPRRDR"
     gene            complement(8437..>8997)
                     /locus_tag="VFE39_15000"
     CDS             complement(8437..>8997)
                     /locus_tag="VFE39_15000"
                     /inference="COORDINATES: protein motif:HMM:NF033748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HET6259945.1"
                     /translation="VACGLQEAPGPSISSPTAVTGAPAPAAGSVAAPIPHSEPVRVEI
                     PVLGVDSELMTLGLEPGGAMEVPPGAYPAGWYSGAPTPGELGPAIIAGHVNWNGHAGV
                     FSRLHEMKPGDQVAVHRRDGSTALFRVDDVAEYPKHNFPTKTVYGNIDHAGLRLITCG
                     GDFDSGARSYLDNIVVYASFVGPATI"
BASE COUNT         1323 a         3184 c         3183 g         1307 t
ORIGIN      
        1 gctgctgcgc catcccgagt tcgccacgcg cgacctgtcg tcggtgcgag gcatctcctc
       61 gggcgcggcg ccgatgccgg tggagatgat cgaggcgctg caccgccgct tcggcgacga
      121 tgtcgtgatc gtcgagggct acgggctgac cgaggtgacc atgggggcca cactcggccc
      181 cgcgtggcgg tcgggcgtac gcaaggtggg caccgtcggg ttgccggtgt acgacacgga
      241 agttcgtatc gtcgctcctg agggcggcaa cctcactccg ctgccgcagg gcgaggccgg
      301 cgaggtgtgc atccggggcc cgcaggtgat ggtcggctac aaggaccggc cggaggagac
      361 cgccgccacc ctggtggacg gctggctgcg caccggcgac atcggtgcca tcgacgagga
      421 cggctacctg tcgatcgtcg accgcaagaa ggacatgctg ctctacaagg gctacaacgt
      481 gtacccgcgc gagctggagg agcttctctt cgcgcagggt gcggtgacgg gagcggcagt
      541 tgtcgggcga ccggatccgg tggtgggcga actcccggtg gccttcgttg tggcgcccgg
      601 gctcggcgac gccgacgccg gcgccctgat ggaggcggtc aacgagcagg tgctcccgta
      661 caagcggctc cgggagctgc acctcgtgga cgagattccg gtgtcggccg ccggaaaggt
      721 gctcaagcgg gagctgcgag agcggttgcc ctgacggagc tctgggagca caccggcacg
      781 gatgagtgac cccacgttaa cgccgggcgt ccggacagcg atgtcccgtt cgtacggata
      841 cccggcatgc gcgcaccgga ccggacaccc gaccgtcatc gccgcttctc gcggcatggc
      901 tgtcgtccca tgtccctgct cccgaagccg gtggtcgcca tgcccactgc cgctgcatcc
      961 cctggtgatg gggtggctgc tgcccggtca cgccgtgatc ccgtcgctcg ggcgggcagg
     1021 caccggcgag caaccacccc gcgcacgctt ctcggcctgg cggtggacgt ggtcggtcgc
     1081 gtggggagcc tcgtgacgct ggcggccctg gtggtgctgg cggcgacggc gggcgcgatg
     1141 gccgacggca tgccggccgg tgggagcgcg accgtcaccg ccaccttcga ccggagcggc
     1201 ccgtgacctc gcgacctcag gccgcttcgg cctgggcgat cggtgactgc agcacgtggt
     1261 cgaccaggcg catcacgagt tgctcgccgc ccaaccggta cccgaactcc tcccaggtga
     1321 gcagctcacg ggagagcagc tctccgacag ggcccgggta cagccgggtg gctcggaagg
     1381 cggccgctcg cagccgcatc ttctcgtggt ggtccatcgg gacggggatc attcggctct
     1441 cctccgcagg tgcttgcaat gcggcgcgct ctcagcggtg agtcgccacc cgtgagcctt
     1501 ccatggtgaa ccaaggtcgg gactacccga cgaatggtac ccgaagagtg actgaggtct
     1561 cacagtgcgc gaaccacggg tatgtgatcg tgcacaggga gattcccatc cgccgccccg
     1621 tcacacctcg ggttagcgtg atcagcatgg tgactccggc cgactccgcc ctgcgtaccc
     1681 gtgatgtgct ctggtccgac atcgaggacg tctacgccgc gatcctcgcc catcccttca
     1741 tctccgggtt gacggacggc acgctgcccc gggagtcgtt ccggcactac atcgtgcagg
     1801 acgcgcacta cctccgcggc tacgcgaagg cactcgcggt gtgcgccgcg aaagcgcccg
     1861 acgaggacgg caccgtgatg ttcgccgcgc acgctgcggg ggccatcgcc gccgaacgcg
     1921 accttcacgc ggcgctgatg ggcgagatgg gctgcacctc ggaggaagcg gcgctggagc
     1981 cgatcgcccc gaccacccag gcctacgtca gctacatgct cgcctcggcg ctcggcgggt
     2041 cgttcgccga ggcggtgggt gctgtcctgc cctgctactg gatctatgcg cgggtcggcg
     2101 aggagctcat ggcccgcagc tcacccgacc cgctgtacgc ccgctggatc gcgatgtacg
     2161 gcagcgagga cttccagtcg gtggtcgacg cggtgctcgc actcaccgac cggatcgggg
     2221 ccgggatgtc cgagggcgag cgcacgctga tgcgcaggca cttccgtacg acgtcccggt
     2281 acgagtggat gttctgggac gcgggttacc gctgcgagac ctggcccgtg tgacgcgtca
     2341 gggccggcgc cctagcgttg gtcgaagcgg tcggggatga ggaagtgcag gatgaatccc
     2401 accaccgcca cgactatcgc accgacgaac gcggcaccga agctctgcac cgtgaacggc
     2461 agcccgatcc cgctggcgat gtacccgacc agcaggaaca gcagcgcgtt gaccacgagc
     2521 ccgatcaggc cgagcgtcag cacgtagaac gggcagccca ccaccttgat cagcggcttc
     2581 agcacggcgt tgaccaggcc gaagatcagg gcgacgacga cgagagtgcc gatccgggtg
     2641 ctggtcgtgc ccgcggtgtc gtcgatcccg gggacgatca gggtggccac ccacaggctg
     2701 acggcgacca ccgccacgcg gatggtaagg gcaagcgtgt tcgacatcgg catcagtcca
     2761 gcagggcctg gttgaccagc gtgcgcagct gggtgcggac agggtaggtg ctcgacggga
     2821 tgagctgtgt gaagaacagc gcggtgatct ccgccacagg gtcgatccag aacgccgtgc
     2881 tggccaggcc gccccacgcg aactcgccct cgtgcgtgag cgccttgttc gcggcggcgt
     2941 cgatgaccac ggagaagccg aggccgaacc cgacgccccg gtagggcgac tcggagaata
     3001 tgggcctgcc gaactgctcc aggtcggcac cgccgggcag gtggttgcgc gtcatgtagg
     3061 cgaccgtgcg cgggccgagc agccgggcgc cgtccaactc gccgccgcgg gcgagcatct
     3121 gcgtgaagcg gtggtagtcg cccgcggtgg agacgagccc gccgccgccg gacaggaacg
     3181 ccggggcgcg agtggcagcg tcgcccatcg atcggttacg caggagcccg cccgcggggt
     3241 tgggcccgta cagcgctgca aggcgctcga catcgtcctc gggcaccgag aatccggtgt
     3301 cgttcatgcc gagggggcga aacacgtgct cggcgaagaa cgcgtcgagc gactgcccgg
     3361 aggccacctc gacgacccgg ccgagcacat cggttgccac cgagtagttc cactccgtgc
     3421 cgggctggaa cagcagcgga acctgcgccc acacctcgca ggcgcccgcg agatccaacc
     3481 cgcgcgggga cccgaactcg aagcccttgg cccggtagat ctcgtcgacg gcgtgggcgt
     3541 gatgccagcc gtaggtgagc ccggcggtgt gggtgagcag gtgccagatc cgcaccggct
     3601 cggtggccgg caccgtgccg ggactggccg ccgacccgcc ctggtagacg cgcatgtcgg
     3661 cgaaggccgg gatgtagcgc gacacggggt cggtgagctc cagcgcaccc cgctcgtaca
     3721 gcatcagcgc cgccacggag gtgatcggct tgctcatcga gtagatgcgc cagatggtgt
     3781 cgtcggcgac gggccgccgc tcctcgaggt cggcgagccc gtagtgactg aggtgcgcca
     3841 cccggccccg gcgcgacacg gcgatcgtcc agccggccag ccgaccgtcg tcgacgtagc
     3901 gccggaaatg cgtgtcgatc cgctccagcc ggccggcgtc catcccgacc tcgcccggat
     3961 cagcgtccac cttgagctgc atcacgcaat cgaccctaca tccggttgat ctcccggccc
     4021 acctcgtcga ccggccgcag acccagcgcc gccagcgctc cggcggcgtc cggatggcgc
     4081 aggacgcggg agatcctgcc gttctcgacg acgaacgccg tggccaaccg caccggctcg
     4141 ccgagctcgc cggtgtgggt gtccagccat cgtgcgtcct gctccacgac ggcaccgccg
     4201 gccccgcaga accagcgcac cggctccatc cggatccccg cccgcccgac ccagtcgagg
     4261 atgagctcac gtccgaaccc cgacccgcgc ggtccaccca cctcgacgtc gctcgtgcac
     4321 aggtccccga ctcgggcggc atcaccgccg ttgaccgcgg cgtgccagtc ctccaccacc
     4381 gcgagctgcc cccctgccat gccggcgagg gtagcggcct cgacgcgcac cgcccaggtt
     4441 ccgcggaccc ggccggacaa cccgttcgtc acgcggagca accctttcgc gtcacgctga
     4501 gcaacccgtt cgcgtcacac tcgggcatga ctcccgctgt tgggagggag gatcgggcgg
     4561 tggccctcca ccccgagccg atcctcgaca tctcgccagc gctgcgcggc gacgcggagg
     4621 tgctccgtgc gctgctgcgg cgccgcgacg caggcatcag ggccgacggg aaccggatcg
     4681 cgctcgtcgt gagcggcggc ggcatgcgcg gcgcgtacgc cggcgggatg gcccacgccc
     4741 tcgacgacgc cgggctcgcc ggttgcttcg acgtgatcta cggcagctcg gcgggtgcct
     4801 acatcggtgc ggcgctgctc ctcggcaacg gccgcgggtc ggcgcacatc ttcttcgagg
     4861 acatggcctg tcgcgcgttc atcgacccgc gccggatcgg cacgcgccgc ccggtggtgt
     4921 cgctggacca cctgatcgac cacgtcctcg tccactcgaa gccgatggcg tgggaccggc
     4981 tggggccgtc accggtgccg ttgcgggtgg tggccacggc ggcggacgac ctgcggccgc
     5041 acgtcctcga tcctgcgaca ccggacgagt ggaaggtggc gctgcgggcg acagcatcga
     5101 tcccgctgct cgccgggccg cccgtcgagc tccacgggcg ccgctggatc gacggctcgg
     5161 tcgcggagcc ggtgtccgtg ctgcgggccc tacaggacgg cgccacccac gtgctcgcgc
     5221 tgctcaaccg caccgctccg gagctgcgcc gggccgacct ggcggcaggc cccgccccgt
     5281 gggcccgcgc gctggaccgg ctcgcgcccg ggctcgggat gatcgcgcag gagagcaacc
     5341 ggcacggccc ggcgctcgcg gtcctcgacg acgccgccca cccctcgcgc aacggggcgc
     5401 acctgctggc actcatgccg gagcaggacc tcggcgtccg cgggctcacc atcgacgtcc
     5461 cgcgggtgac gcaggccgca gcagcgggat acgcgaccgt cacttcggca ctgtgccgcg
     5521 tccggagctc tcaatgatcg acgcgatccg tacctgagcg ccccgccggg gacactggtg
     5581 gacgcaccgg aacgatcacg tgggcacgcg gccttgaccc gactcgtcgc caccaggagt
     5641 gttgccgggt ggacgctgat gtcatcgtgg tcggcgcggg actcacaggt ctcgtcgcgg
     5701 cagccgagct ggccgacgcg ggcaggcggg tgctgctgct cgaccaggag cccgagacgg
     5761 ggctgggcgg gcaggcgttc tgggccttcg gcggcctgtt cctcgtcgac tcccccgaac
     5821 aacgccggtt gggcgtccat gactccttcg acctggcgat gcaggactgg ctgggcagcg
     5881 ccgccttcga ccgcggcgtc gacgacccga cgggcgagga cttctggggc cgccagtggg
     5941 cgacggcata cctcgacttc gccgcaggtg agaagcgttc gtggctgcac cggcaggggg
     6001 tgcgctggtt cccggtcgtg ggctgggcag agcggggcgg ctacctggcc gacggccacg
     6061 gcaactccgt gccgcgcttc cacatcacct ggggtacggg gccgggtctc ctggccccgt
     6121 tcgagcgccg ggtgcgggag gccgcggcga aggggctcgt cgagctgcgc ttccggcacc
     6181 gggtggacgg cttgacggtc accgacggag tggtcgacgg cgtccacggt gcggtgctcg
     6241 agccgagcag cgccgcgcgt ggcacggcga gctcgcgcaa cgaggtgggc gagttcgaac
     6301 tgcacgcgca ggccgtcgtc gtcacctccg gcgggatcgg tgccgaccac gagatggtgc
     6361 gcgccaactg gccgtcccgc ttgggccgcc cgccccgcta catgatctcg ggggtccccg
     6421 agcacgtcga cggccggatg ctggcgatca ccgagcgcgc gggcgggcgg atcgtgaacc
     6481 gcgaccggat gtggcactac accgagggcg tccacaactg gggcccgatc tgggcccgcc
     6541 acgggatccg gatccttgcc ggaccctcct cgctctggtt cgacgcgcgg ggccggcgct
     6601 tccccacacc caacttcccc ggcttcgaca ccctcggcac gctcgaagcg attacccgga
     6661 ccggctacga ccactcctgg ctggtgctca cccagaaggt catcgagaag gagttcgcgc
     6721 tctcgggctc ggagcagaac ccggacctga ccggcaagga cgtccgggcc accctcggcc
     6781 ggatccggcc gggagcaccg gccccggtcc tggcgttcat gaacaagggc gtggacttcg
     6841 tcgtcgccga cacgctcgcc gagctcgtcg caggcatgaa caagctcact cggcacaaca
     6901 ctggcggagc ggacggcgac atcgacgagc cgctgatcga tctcgctgag ctgaagcgcc
     6961 agatcgtcgc ccgcgaccgg gagatcgaca atcccttcac gaaggacctg cagctcgccg
     7021 ccgtgcattc agcacgccgc taccggggcg acagactcgc ccgcattgcg acgccgcaca
     7081 agatcctcga cccggcgaac ggtccactga tcgccgtgcg gctcaacatc ctcacccgca
     7141 agacgttggg gggcctgcag accgacctgt cgtgccgggt gctgcgcgcc gacggcaccc
     7201 cgttccccgg cctctacgca gcaggggagg tcgccggctt cggcggcggc ggcgtgcacg
     7261 ggtaccgctc cctggagggc acgttcctcg gcgggtgcct gttcaccgga cggcaggcag
     7321 gcagggccgc ggggctctcg gtgtcctgac ggccggagac accggcgtga tcgcgtcacc
     7381 gtcgatcacc agctggtgac cgagctcccc gagcttgtgc tcgaaccgct cgttgcgccg
     7441 gatctggtcg atcgcccgcc gcttcgcgac tgccatgagc cacgcacctg ggctgcgcgg
     7501 gacgccggac tccggccact gttcgagggc ggcgacgagg gcgtcctgcg caagctcctc
     7561 ggcgaccccg acatcacgat cagccgggcg agcccggcga tcagccgcgg cgcctcgatc
     7621 cgccagaccg cctcgatcgt cctgttggtc tccgtcacgg cgagccatca gagcagccct
     7681 cccgcgtatg acgctttcgc cacgcgaccg acgacgcggt cggggcgagt tcctcgcgtc
     7741 ggcgccgaac acgatcatgt tgatcgaagg tcccggcgtg cacgaccgcg tacctgcggc
     7801 aggtggcggc ggagttccag gccgggcact gggtgcgggt cgggggcgcc actcggcgcg
     7861 cccggacacc tcgtgatcga gatcgtcgcc gcaccgtgta gcgattgaac gatcatcgcc
     7921 gagcaccagt catggcgtcg ccgactgcga gggcagagat gaccgatcgt ggggcccgga
     7981 gggacgcccg gccgagccgg gccgctccgg tcaccgcagt cgtgctgggc acaggcgacg
     8041 gcctcctgct gatcgtcctc gccaccgctg ccgcagggaa tctcgaccgc tggagcgtca
     8101 ccgacgccgt cagcttcgcg gtcgccgtat tcagtgggct gaacctcctg tggggcgccg
     8161 cgaccctcct caggaagatg ccgatcggcc ggctgctgct gaccacgacc gctgggacca
     8221 tcggcgtcgg tgcggtggtc ctgctcctcg tccagcccgc accgtccggc cggctcgtcg
     8281 acgtggtgac cggtgtgctg ctcgtcggcc tgcagacggt gatcatctcg tgcgcgatga
     8341 gccggcccac gttgttgtgg atcgtcggtg gaccaggccc gcggcgggac cgctagacgg
     8401 cggtggcggg cgcgtcggag cccgtgtcgc cgtcactcag atcgttgccg gcccgacgaa
     8461 gctcgcgtag acgacgatgt tgtcgaggta gctgcgggcg ccgctgtcga agtcaccgcc
     8521 acaggtgatg agcctcaacc cggcgtggtc gatgttcccg taaaccgtct tcgtcgggaa
     8581 attgtgcttc ggatattcgg ccacatcgtc cacccggaag agcgccgtgg agccgtcacg
     8641 ccggtgcacg gcgacctggt ctccgggctt catctcgtgc agcctggaga acacaccggc
     8701 atggccgttc cagttgacgt gcccggcgat gatggccgga cccagctcgc cgggagtcgg
     8761 tgctccgctg taccaaccgg cggggtaagc gcccggtggc acttccatcg ccccgcccgg
     8821 ctccagaccg agtgtcatca gctcggagtc gactccgagg acggggatct cgacgcgtac
     8881 cggctcggag tgcgggatgg gcgcggctac tgaccccgca gcaggagcag gcgcaccagt
     8941 gaccgccgtg ggagaactga tcgacggacc gggcgcttcc tgcagcccac aggcgac
//
LOCUS       DASRSC010000304         7914 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5853223,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000304 DASRSC010000000
VERSION     DASRSC010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7914
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5853223"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VFE39_15005"
     CDS             <1..1213
                     /locus_tag="VFE39_15005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HET6259946.1"
                     /translation="PDTSGPDTSGPDSSQPDASAPDGSVVGSSRPDGSAPDGSAPDGS
                     PPDISPNGSSTNSNGNGSNGGGPEGGGDAGGSPWAAPEWMPRLAEPASGAAAAAPGVA
                     ITHPGAQATPSPYPQHPYQPGQYPPGTYPPASYPPASYPSASYPSAVHPAGSFPGGPP
                     PLRQPPRGISAAGPARTHRWGLGAYLLVEAVFLLTSVLVGWVVMGGGPPAAGGLIAAL
                     AVPTVLAAGTAVVITRLRGNGPRTDLRLQWSWGDVGLGLAFGLGGLALTVPASLVYVS
                     IVGQDASSAVGDVFGPIRTGPVLAALVLVTVVFVAPLCEEIVYRGLLWGGVERLANRW
                     VAFGVTTLLFAMAHFEFTRTPLLLVVALPIAAARLYTGRLLPSIVAHQVNNLLPGIVL
                     MLGLLGLMPAS"
     gene            1406..1858
                     /gene="rpsP"
                     /locus_tag="VFE39_15010"
     CDS             1406..1858
                     /gene="rpsP"
                     /locus_tag="VFE39_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET6259947.1"
                     /translation="MAVKIKLMRLGKIRQPYYRIVVADARNRRSGRAIETIGKYHPKN
                     EPSLIEVDSDRALYWLGVGAQPTDPVQHLLEITGDWQKFKGLPGTEGTLKPQPEKVDK
                     LARFQAALAAAGEEPTTEATTPRKRGDRRPKADSAEGEATTATEATES"
     gene            1855..2094
                     /locus_tag="VFE39_15015"
     CDS             1855..2094
                     /locus_tag="VFE39_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HET6259948.1"
                     /translation="MSVLADALEHLVWGIVDHPDDVQVELVTTRRGRTLEVRVHPEDL
                     GKVIGRGGRTATALRTVVGGIGGRGVRVDVVDTDR"
     gene            2072..2646
                     /gene="rimM"
                     /locus_tag="VFE39_15020"
                     /pseudo
     CDS             2072..2646
                     /gene="rimM"
                     /locus_tag="VFE39_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676803.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
     gene            2775..2990
                     /locus_tag="VFE39_15025"
     CDS             2775..2990
                     /locus_tag="VFE39_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6259949.1"
                     /translation="MTDATKVAAAASSRDPAVGLAAVASLRGLLESLEELQVTNARAQ
                     GWSWQQIADVLRVSRQAVHKKYRRFGF"
     gene            3002..3565
                     /locus_tag="VFE39_15030"
     CDS             3002..3565
                     /locus_tag="VFE39_15030"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HET6259950.1"
                     /translation="MFERFTEKARTVVIGAQKQARERGADTIRSEHLLAALFDVPDNL
                     AVTVLAALGVDRDVVESEIGKLRPDGRPTPDAEALATLGIDLDEVRRQVEEAFGPGAL
                     DRTRAASSRGWGAHIRFDRTAKKVLELALREALLLKHNYIGTEHILLGMLQPANGAAH
                     DILVSHGVRQDATRILVEEFVRGRRAG"
     gene            3589..4335
                     /gene="trmD"
                     /locus_tag="VFE39_15035"
     CDS             3589..4335
                     /gene="trmD"
                     /locus_tag="VFE39_15035"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET6259951.1"
                     /translation="MRIDVVTIFPAYLAPLREALLGRAIGAGLVDLAVHDLRSWTRDV
                     HQAVDDSPYGGGPGMVMRPQVWGAALDTVLEQNGPARLVVPTPAGRPFTQDTAEAWAT
                     EPRLVFVCGRYEGIDQRVMDDAAARMPVDEVSIGDYVLVGGEAAVLVMVEAVVRLLPG
                     VLGNPASAQQDSFSDGLLEGPAYTRPETWRGLSVPEVLRSGNHAAIARWRRDRALERT
                     AERRPDLLAALPEEALDNADRAFLADRSGG"
     gene            4481..4840
                     /gene="rplS"
                     /locus_tag="VFE39_15040"
     CDS             4481..4840
                     /gene="rplS"
                     /locus_tag="VFE39_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET6259952.1"
                     /translation="MNTLDALDAQQLRSDIPAFRPGDTLKVHVKVVEGTRSRIQVFQG
                     VVIRRQGAGVRETFTVRKVSFGVGVERTFPVHSPNLERIELFTRGDVRRAKLYYLREL
                     RGKAAKIKEKRETPSAS"
     gene            5234..6202
                     /gene="lepB"
                     /locus_tag="VFE39_15045"
     CDS             5234..6202
                     /gene="lepB"
                     /locus_tag="VFE39_15045"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET6259953.1"
                     /translation="MHHGLIDGDDDQSSEVPGRHRRRAGESPADRPSVDYQNQADTGG
                     RKARSGRRRRPTFWRELPLLIVVALVLTFLIQTFLAKVYVIPSGSMETTLHGCTGCNN
                     DRVLVDKITYRFADPAPGDVVVFRGPDSWSSEISVQEPTSPVTRALQGFGSLIGLAPP
                     DEKDFVKRVIAVGGQTVSCCDSRDRVMVNGEPLNEPYIYYLPEAGPPRQSPFGPITVP
                     EGQLWMMGDSRNNSADSRVPGHGPVPVANVIGKARVIVLPLSRLSWIQSVNPQSNAVG
                     MPAPGAPAGAPLALGLLGTLPLAAGRRRLHRRDAELRSFLPGHPGP"
     gene            6269..7099
                     /locus_tag="VFE39_15050"
     CDS             6269..7099
                     /locus_tag="VFE39_15050"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET6259954.1"
                     /translation="MPARPALPRTGPRRPARPALPPELRPARAVVRRSAGSWTLQTTL
                     HRHGLGPVAGVDEAGRGACAGPLVVAACVLRPGDAKRLDGLTDSKLLTPAAREQYFAL
                     ITRWAQDHAVVVIPPEEVDRRGVHVANIEGMRRAVAALAGRPGYVLTDGFPVRGFGSP
                     ALAVPKGDQVAACVAAASVLAKVTRDRLMVGLDGEFPQYGFAEHKGYCTPAHDAALAA
                     HGPSRVHRFSFVNVRAARGDDVSALEAAAAVSLTAALVHNGSLTVGLSDVGVEGGLEL
                     "
     gene            7096..7419
                     /locus_tag="VFE39_15055"
     CDS             7096..7419
                     /locus_tag="VFE39_15055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 domain-containing protein"
                     /protein_id="HET6259955.1"
                     /translation="MSAEDLEKYETEMELTLYKEYRDIVAQFSYVVETERRFYLANSV
                     DVAARNADGEIYFEVRMSDAWVWDMYRPARFVKHVRVLTFKDVNVEELDKPDLRLPEG
                     NQFGS"
     gene            7777..>7914
                     /locus_tag="VFE39_15060"
     CDS             7777..>7914
                     /locus_tag="VFE39_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HET6259956.1"
                     /translation="MAAKDVLGRHGEDLAARYLEQRGLVVLSRNWRCRHGELDLVATD
                     AD"
BASE COUNT         1123 a         2838 c         2817 g         1136 t
ORIGIN      
        1 gcctgacacc agcgggcctg acaccagcgg gcccgacagc agccagcccg acgccagcgc
       61 gcccgacggc agcgtggtcg gtagcagccg gcccgacggc agcgcgcccg acggcagcgc
      121 gcccgacggt agcccgcccg acatcagccc gaacggcagc agcaccaact ccaacgggaa
      181 cgggtcgaac ggcgggggtc cggagggcgg cggtgacgca ggcggctccc cgtgggccgc
      241 accggagtgg atgccgcggc tcgcggagcc ggcctcgggg gcggccgccg cagctcctgg
      301 tgtcgcgatc acccatcccg gagcccaagc gacgcccagt ccgtacccac agcacccgta
      361 ccagccaggg cagtacccgc cgggcacgta cccacccgcg tcctacccac ccgcgtccta
      421 cccgtcggcg tcctacccgt ccgcggtcca ccctgcgggg tccttcccgg gcggcccgcc
      481 gccgctccgg cagccgcccc gagggatatc cgctgcaggc ccggcccgga cccaccgctg
      541 gggcctgggt gcgtacctac tcgtcgaggc cgtgttcctg ctcacctccg tgctggtcgg
      601 ctgggtggtc atggggggcg ggccgcctgc tgcgggtggc ctgatcgccg cgctcgcggt
      661 gcccaccgtg ctcgcggccg gtaccgcggt agtgatcacc cggctgcgcg gcaacggacc
      721 gcggacggac ctgcgactgc agtggtcgtg gggcgacgtg gggctgggcc tcgccttcgg
      781 gctcggcgga ctggccctga cggttccggc gtcgctcgtg tacgtctcga tcgtcgggca
      841 ggacgcctcc tcggcggtcg gcgacgtgtt cggccctatc cggaccgggc cggtgctcgc
      901 ggcgttggtg ctggtgaccg tggtgttcgt cgcgccgctc tgcgaggaga tcgtctaccg
      961 cgggctcctg tggggcggag tggagcggct cgcgaaccgc tgggtcgcgt tcggcgtgac
     1021 gacgctgctc ttcgccatgg cccacttcga gttcacgcgc acgcccctgc tgctcgtcgt
     1081 cgcgctcccg atcgcggcgg cacgcctcta cacgggcagg ctgctcccga gcatcgtcgc
     1141 ccaccaggtc aacaatctgc tgccgggcat cgtgctgatg ctcgggctgc tggggctcat
     1201 gccggcgagc tgaggccgcc cccgggcgaa ccaggcggct ctgtgcaccg tcgccggtcg
     1261 tcgtctggca gaatggtgga tcggctccgc ggttggccct ctacttgccg cggccacaac
     1321 acgtcgacga agcgctgccc gtaaccccac gcgagccgcg cggactcacc cgaagcacga
     1381 tgatccgcga ggagcaacga caagcgtggc cgtcaagatc aagctgatga ggctgggcaa
     1441 gatccgtcag ccgtactacc gcatcgtcgt ggccgatgcc cgcaaccgcc gcagcgggcg
     1501 ggccatcgag accatcggca agtaccaccc caagaacgag ccgagcctga tcgaggtcga
     1561 ctccgaccgg gcgctgtact ggctgggtgt cggcgcgcag ccgaccgacc cggtgcagca
     1621 cctgctggag atcaccggcg actggcagaa gttcaagggc ctgcccggca ccgagggcac
     1681 cctgaagccg cagccggaga aggtcgacaa gctcgcccgt ttccaggcgg cgctcgccgc
     1741 ggcgggcgag gagcccacca ccgaggccac cacgccgagg aagcggggcg accgccggcc
     1801 caaggccgac tccgccgagg gtgaggccac cacggccacc gaggccaccg agtcgtgagt
     1861 gtcctcgcgg acgctctcga gcacctcgtc tggggcatcg tcgaccaccc ggacgacgtg
     1921 caggtagagc tcgtcaccac gcgccgggga cgcaccctcg aggtgcgcgt gcaccctgag
     1981 gacctcggca aggtgatcgg ccggggcggc cgcacggcca ctgcgctccg cacggttgtg
     2041 ggcggcatcg gaggccgggg cgtgcgggtg gatgtcgtcg acaccgatcg gtaacggcat
     2101 cgagcgagcg cggcgaggga gcatcgacac cgagctgctc gtcgggatcg ccgccagggt
     2161 ccacggcctg cgcggcgagc tcgtcgtcga cgtgcacacc gactcgcccg acgatcggtt
     2221 cgcccccggt gccgtgctca ccgcgcgccg cgccggggcg ccggacggca cggtcaccgt
     2281 cgagtcctcc cggccgcact ccggccggct gctggtgcgt ttcgccgagg ttcccgaccg
     2341 tgggccgcag aggcgatgcg tggggccagg ctgctgatca aggcggatgc actggcgcca
     2401 cccgccgatc cggaggagtt ccacgtccac cagctcgagg gcctgcgggc cgagctgacg
     2461 gacggctcgg tcgtggggac ggtccgtgag gtcgtgcacg ggccggccgg ggagctgctc
     2521 gtgctcgccc gtcccgagct gccggatgca ctggtgccgt tcgtgcgcgc gatcgtgccc
     2581 accgtcgacc ttgacggcgg ccgggtcgtc ctcacgcctc ccgaaggtct gctcgacctg
     2641 gactgaactc tccgatccct ccttccaccg gggtgacatt cctgacgctc gacgtcagcg
     2701 gtgtcacccc gctggcattt ctgcgccgtc aactagggtt gacagtcgtc gcgttgtcaa
     2761 cgtaggttga caccatgacg gatgcgacga aggtcgcggc ggcggcgtcc agtagggacc
     2821 cggccgtcgg gttggcggct gtggcctccc tgcggggact gctggagtcg ttggaggagc
     2881 tgcaggtcac caacgcgcgg gcgcagggct ggtcctggca gcagatcgcg gacgtgctgc
     2941 gggtgagcag gcaggccgtg cacaagaagt accgccggtt cgggttctga ggggagcgga
     3001 catgttcgaa cgtttcacgg aaaaggcgcg gaccgtggtg atcggcgcgc agaagcaggc
     3061 gcgggagcgg ggcgcggaca ccatccgcag cgagcacctg ctcgccgcac ttttcgatgt
     3121 gccggacaac ctggcggtca ccgtgctggc cgccctcggg gtcgaccgcg atgtggtgga
     3181 gtccgagatc gggaagctac gcccggacgg gcgcccgacg cccgacgccg aggcgctggc
     3241 cactctcggt atcgacctgg acgaggtgcg ccgccaggtg gaggaggctt tcggtcccgg
     3301 cgcgctggac cgcacgcgcg ccgcgtcgag ccgcggctgg ggcgcgcaca tccggttcga
     3361 tcgcacggcg aagaaggtgc tggagctggc gctgcgcgaa gcactgctgc tcaagcacaa
     3421 ctacatcggg accgagcaca tcctgctcgg catgctgcag cccgcgaacg gggccgcgca
     3481 cgacatcctc gtctcgcacg gggtgcggca ggatgccacg cggatcctcg tcgaggagtt
     3541 cgtccgcggc cggcgggccg gctgatccgt ccgcgtacgg ttccccgggt gcggatcgac
     3601 gtcgtcacca tcttcccggc ctatctcgcc ccgctgcggg aggcgctgct gggccgggcc
     3661 atcggagccg ggctcgtcga cctggcggtg cacgacctgc ggagctggac acgtgacgtc
     3721 caccaggccg tcgacgactc cccgtacggc ggggggccgg gaatggtgat gcgcccgcag
     3781 gtgtggggtg cggcactcga cacagtgctg gagcagaacg gcccggcgcg gctggtggtg
     3841 cccaccccgg ccggcaggcc cttcacccag gacacagcgg aggcttgggc caccgagccg
     3901 cggctggtgt tcgtctgcgg gcgttacgaa gggatcgacc agcgggtgat ggacgacgcc
     3961 gccgcgcgga tgcccgtcga cgaggtgagt atcggcgact acgtcctcgt cggcggcgag
     4021 gcggcggtgc tggtgatggt cgaggcggtg gtgcgcctgc tgcccggcgt gctcggcaac
     4081 ccggcgtcgg cgcagcagga ctccttctcc gacggtctcc tggagggccc ggcctacacc
     4141 cgcccggaga cctggcgcgg gctctcggtc ccagaggtgc tgcgcagcgg caaccacgcg
     4201 gcgatcgcgc gctggcgccg ggaccgggca ctggaacgga ccgccgagcg tcggccggac
     4261 ctgctcgccg ctctgcccga ggaggcgctc gacaacgccg accgtgcctt cctcgccgac
     4321 cgttcgggcg ggtgatttca ctctgcccga tcgcgtctgg cactatggac gggttgccag
     4381 cgctcgacgg acacgtcggg cctgtccggc gccaccaacc ttgacaatgg gtcggcgccc
     4441 ccgcacgacc gctatcacgg accgaggacg gatcttcgcg atgaacaccc tggacgccct
     4501 ggacgcacag cagctgcgct ccgacatccc cgccttccgg ccgggagaca cgctcaaggt
     4561 gcatgtgaag gtcgtcgagg gcacgcgatc gcggatccag gtgttccagg gcgtcgtcat
     4621 ccgtcgccag ggcgcaggcg tccgcgagac cttcacggta cgcaaggtgt cgttcggcgt
     4681 cggcgtggag cgcaccttcc cggtgcactc gccgaacctc gagaggatcg agctgttcac
     4741 ccgcggcgac gtccgccggg cgaagctcta ctacctccgt gagctccgtg gcaaggccgc
     4801 gaagatcaag gagaagcgcg agactccgtc cgcgtcctga ccagcccggc acggttgacg
     4861 tagcctcgtg ggcgtggccc tacccgagct ccccgacgac cgacgggccc cgccccggcg
     4921 gtactcgccg gatcagccgg tacccgtccg cccgcccggg cggcagccgg ccggtcccga
     4981 gcaggtggcg ccaccggttc caggagctcc ctaccccggg actccggccg gacgaggggt
     5041 gccgcagccc aacggtgcgc cgtcgctacc cggagcgccg ccgaacgggg ccgccgttcg
     5101 cggcggaccg aacggggcgg gttcgctacc ccgcgggcgc cccgatgccg ggcagccggc
     5161 caacgggagc cgaacccccg gcaggcccgc gcccccgcgg cgtcgcgatc cttctgacga
     5221 cgagccgaca gacgtgcacc acggtctgat cgacggggac gacgaccagt cctccgaggt
     5281 ccccggccgg caccgccgac gggccggcga gtcacccgcg gatcgcccgt ccgtcgacta
     5341 tcaaaaccag gccgacaccg gcggccgcaa ggcccgttcc gggcgccgta gacggccgac
     5401 gttctggcgg gaactaccgc tgctcatcgt ggtggcactg gtgttgacgt tcctcatcca
     5461 gaccttcctc gccaaggtct acgtcatccc gtccggctcg atggaaacca cgctgcacgg
     5521 ctgcaccggg tgcaacaacg atcgtgtgct cgtcgacaag atcacctacc ggttcgccga
     5581 cccggccccg ggcgacgtag tggtgttccg cggcccggac agctggagca gcgagatcag
     5641 cgtccaggag ccgaccagtc ccgtgacccg ggcactgcag gggttcggat cgctgatcgg
     5701 cctcgccccg cccgacgaga aggacttcgt caagcgggtg atcgcggtcg ggggccagac
     5761 cgtgtcgtgc tgcgactccc gggaccgggt gatggtcaac ggtgagccgc tcaacgagcc
     5821 gtacatctac tacctgcccg aggccgggcc gccgcggcag agtccgttcg ggccgatcac
     5881 cgtgcccgag gggcagctgt ggatgatggg cgacagccgc aacaactccg ccgactcccg
     5941 ggtacccggc catggtccgg tcccggtggc gaacgtgatc ggcaaggcac gggtgatcgt
     6001 gctgccgcta tcgcgtctga gctggatcca atccgtgaat ccgcagtcca acgcggttgg
     6061 catgcccgcc cccggtgccc cagccggagc gccgctcgct ctcgggctgc tcggcacgct
     6121 gccactggcg gcagggcggc ggaggctgca ccggcgggat gcggaactgc ggagtttcct
     6181 ccccggtcat cccggcccct gatccgggcc gccgaccctc ggccccgtgt cgcccgcccc
     6241 tgacagcccg gtggagactg ctgacctgat gcccgctcgc cctgcccttc ctcgcaccgg
     6301 gccccgccgt cccgcacggc cggccctgcc ccccgagctg cgccccgccc gcgccgtcgt
     6361 gcgtcgctcc gccgggagct ggaccttgca gaccacgctg cacaggcacg gcctcggtcc
     6421 ggtagccggc gtcgacgagg ccggtagggg cgcctgcgcc ggaccgctcg tcgtcgctgc
     6481 ctgcgtcctg cggccgggcg acgccaagcg gctcgacggc ctcaccgact ccaagctgct
     6541 caccccggcc gcgcgcgagc agtacttcgc gttgatcacc cgatgggcgc aggaccacgc
     6601 cgtggtcgtc atcccgccgg aggaggtgga ccggcggggc gtgcacgtcg ccaacatcga
     6661 gggcatgcgc cgcgcggtgg ctgcgctggc gggccgcccg ggatacgtcc tgaccgacgg
     6721 cttcccggtg cgcggcttcg gcagtcccgc gctcgcggtg ccgaagggtg atcaggtggc
     6781 ggcatgcgtc gcggccgcct cggtgctggc caaggtcacc cgggaccgcc tcatggtcgg
     6841 gctcgacggg gagttcccgc agtacggatt cgccgagcac aagggctact gcacgccggc
     6901 gcacgacgcc gcgttggcgg cgcacgggcc cagccgagtc caccggttct cgttcgtcaa
     6961 cgtgcgcgcc gcccgcggcg acgacgtctc tgcactcgag gccgctgccg ccgtgagcct
     7021 caccgcggcc ctggtccaca atgggtcact caccgtcggg ctgtccgacg tgggtgtcga
     7081 aggagggctg gaactgtgag cgccgaggat ctcgagaagt acgagaccga gatggagctc
     7141 acgctctaca aggagtaccg ggacatcgtc gcccagttct cctacgtcgt ggagacggag
     7201 cgccgcttct acctggccaa ctcggtggac gtcgccgcac gcaacgccga cggggaaatc
     7261 tacttcgagg tccggatgtc ggacgcgtgg gtgtgggaca tgtaccggcc ggcgcgcttc
     7321 gtcaagcacg tcagggtgct gacgttcaag gacgtcaatg tggaggagct ggacaagccg
     7381 gatctgcgac tgcccgaggg caaccagttc ggatcttgac gtcggtttcg ccagtcggat
     7441 cgtcccgtat ttcgcgatat gcatcattgt gtttgtccac ccccgcgatg aatagcatgt
     7501 ccagcggact gtgcgacgaa aggtgtccga ctccgggaat gttcgaaatc cctgcagagc
     7561 acatccacga cacgagggtg ctgcggacgt ggttcgccgg tcgcctcgtg cgcgctgcca
     7621 cgacctgagc acggcagcct gagcccatac acctccgctc cgacggagtg gtcgtgcacg
     7681 gggccgtcgg cacgcagctg tccccgtcgc cctcgatccg tccacagttg cgagaaccgt
     7741 ctgtcccgcg ccgcccccgg cgtcgatcgt gggggcatgg cagcgaagga cgttctcggt
     7801 cgccacggtg aggacctcgc ggcgcggtac ctggagcaac ggggcctggt ggtcctgtcg
     7861 cgcaactggc ggtgccggca cggcgagctc gatcttgtcg ccaccgacgc cgat
//
LOCUS       DASRSC010000305        11977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3071926,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000305 DASRSC010000000
VERSION     DASRSC010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11977
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3071926"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /locus_tag="VFE39_15065"
     CDS             complement(<1..140)
                     /locus_tag="VFE39_15065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET6259957.1"
                     /translation="MDVQVVDHPLAKARLSELRDARTDNPTFRAALRDLTLMLIYEAT
                     RDA"
     gene            complement(247..687)
                     /locus_tag="VFE39_15070"
     CDS             complement(247..687)
                     /locus_tag="VFE39_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6259958.1"
                     /translation="MEEGTSKSPTHSCGTDGPYTDAYQWDTREDCEVRQILDRIGDKW
                     SLLVIALLDRRRLRFTELRREIDGVSQRMLTVTLRQLERDGLVHRTVHAVVPPRVDYE
                     LTSLGVTLHETIQALVMWTESHQNEIAAARAAYDARTAAAANAQ"
     gene            789..2171
                     /locus_tag="VFE39_15075"
     CDS             789..2171
                     /locus_tag="VFE39_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259959.1"
                     /translation="MSGRAWGLLLVLCGAIFLEGIDVSMMGVALPSIRAELGMSTSSL
                     QWVVSAYLLGYGGFVLLGGRAADLLGRRRMFLTWLVVFVAFSGLGGLATEGWVLVVAR
                     FVTGVAAAFMAPAGLSIITTTFAEGRARNRAVLVYAGAAAGGFSLGMVVGGLLTAIDW
                     RWVFFAPVLVAAVILVAAVLLIPREQRPSRIRIGEFDAAGTAGLTGAMLLLVVTVVRA
                     PDVELGTTLGTLAASIALFVAFVLIERRSSAPLVRFGILRSAPLLRANAGALLLVGSF
                     MGFQFTVVLYLQELRGWSEVETGLALAVAGIDAVLAPTLTPWLVNRFGNARVVVGGLL
                     LSAVAYLLFLPVGLDWSFVAMLPALLVLGVAFALAYGPLTIAATNGIADEEQGLAGGL
                     LTTSFQFGAALGLAVVAAVIVANTADDSPQAVFDGFRAGLVVPLVAAVLGAALTASGL
                     RRRTAPARTA"
     gene            complement(2289..3872)
                     /locus_tag="VFE39_15080"
     CDS             complement(2289..3872)
                     /locus_tag="VFE39_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259960.1"
                     /translation="MSAVRAEPAGGPSRRSTGGAGWGCHRPGIGAQLAPVDNRFSWLR
                     PGPLIASRNDVLARWFGDPTDDQRREWLRRLDAYARAEGVPAPPRDRLVARFADRDRI
                     SVAVLGDPGEGDASQYVVVPVLERVAADTDLGIVCSDVVYPAGGVRAYADRFYRPYAG
                     YPAPLYALPGNHDWYDGLTGFMTAFCDAPPDAGAPSPAMAGPLWKRMLRQLLWRRAPK
                     ATAEDVADMRWFRNADSQQAGQPGPYCAIDAGPVRLVLIDVGMDGHIDSDQAEWLCAV
                     SAGDRPKILLTGRPIYTYAHVQACPIEGGGDVNDIVTDPANHYVAAIGGDDHNYQRYP
                     VLQPDGRTIQYVVAGGSGAYLSATHQIPNVDRLAPAVHEEAFRCYPLRGDSLAHFSRR
                     YDKLLGFGLGRLVISPDDATTLMAERVGIAPPRPGSPAVSERARRAADRIFPLPARGT
                     APAHNWMSLLFDSNAVPQFKSFLRLDATRDEIVLSCWAATGCREHELDPVLEDRVRAT
                     RQPDDRWVWTSETPGRPVP"
     gene            complement(3869..4780)
                     /locus_tag="VFE39_15085"
     CDS             complement(3869..4780)
                     /locus_tag="VFE39_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HET6259961.1"
                     /translation="MRTLRTPDERFADLPGFDLDPRYADVPDGDGHTLRMAWVEAGPA
                     DGPVVLLLHGEPSWSFLYRTMIPVLTAAGLRAVAPDLVGFGRSDKPAEISDHSYARHV
                     EWLRALAFDVLDLRDVTLVGQDWGGLLGLRLVAEHLDRFAAVVAANTGLPTGDHDMPE
                     VWWQFRRAVEKAEHLDVARLVQSGCRTRLPPEVRAGYDAPFPDESHKAGPRAMPGLVP
                     TRPDDPATEANRAAWATLSRSEIPFLCAFSDGDPITAAMEPILRRTMPGTAGREHPTI
                     TGAGHFLQEDAGPALAAEVVRFVAGSE"
     gene            5349..5585
                     /locus_tag="VFE39_15090"
     CDS             5349..5585
                     /locus_tag="VFE39_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259962.1"
                     /translation="MPENRITDEATLVAAAVKLTQCDGYVVLAVDPQTGEVDAHGPFD
                     GLTATLKADQLRRDFDRGGLEDVTVGVVRLHSST"
     gene            5907..6869
                     /gene="deoC"
                     /locus_tag="VFE39_15095"
     CDS             5907..6869
                     /gene="deoC"
                     /locus_tag="VFE39_15095"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET6259963.1"
                     /translation="METRAGAGFGDAVRDEASLRRFLRGLPGVDQVGLERRSATLASR
                     SIKKESKLQALDMAISMIDLTTLEGADTPGKVRSLCAQARRPDPEHPDVPPVAAVCVY
                     PDLAGVAVEALRGSAVAVAAVATAFPSGRSSRAVKLADTAFAVDAGASEIDMVIDRGA
                     FLTGRYGAVFDEIVAVKQACGSGERRAHLKVILETGELAGYDDVRRASWLALLAGADV
                     IKTSTGKISPAATLPVVQVMLQAVRDWHRATGELRGVKAAGGIRTAKDAIRHLVAVNE
                     VAGPAWLTPELFRFGASSLLGDLLRQRRTQLDGHYESTDRIGSA"
     gene            6866..8299
                     /locus_tag="VFE39_15100"
     CDS             6866..8299
                     /locus_tag="VFE39_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010315835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HET6259964.1"
                     /translation="MMDWTYAPAPESASLANLQPSYRPFVDGEFVDGGGQPLKTLNPA
                     TEEVLAEVGTASTEDVDRAVAAAHRAFETTWGPMPGAERAKYLFRIARVVAERARELA
                     VLETLDNGKPIRESRDVDVPTASAHLFHHAGWADKLGYAGLGPDPRPLGVVGAVIPWN
                     FPLLMAAWKIAPALACGNTIVLKPAETTPLTALVLAEIAAEAGLPPGVLNVLPGAGDV
                     GAALVGHEGLDKVAFTGSTAVGKEIQRRLAGTGRRLTLELGGKAANIVYDDAPLDQAV
                     DGVVDGIFFNQGHVCCAGSRLLVQESIADEFEALLRARIETLRVGDPLDKNTDVGAIN
                     SRAQLDRIVDLAAAGDAEGARRWTSSCPLPERGLFFPPTVFSGVEQTMRVAREEIFGP
                     VLSVLTFRTPDEAVAKANNTPYGLSAGIWTEKGAKALWTAQRVRAGVVWTNTFNRFDP
                     TAPFGGYGESGFGREGGRIGLEAYLDA"
     gene            8546..9130
                     /locus_tag="VFE39_15105"
     CDS             8546..9130
                     /locus_tag="VFE39_15105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET6259965.1"
                     /translation="MNDRPLRLVVIIGSTREGRFAPTVARWITGEARQHADLDVDVLD
                     LADTALPDRLTGFGQPPPPEVAAVAPRLAAADAFVVVTPEYNHSYPAPLKTLIDWHLA
                     EWQAKPVGFVSYGGMSGGLRAVEHLRAVFAEVHATTVRETVSFHGAWARFGEDARPLD
                     ELECSAAAKTMLDQLAWWADALRTARTRRPYNAG"
     gene            9199..10548
                     /locus_tag="VFE39_15110"
     CDS             9199..10548
                     /locus_tag="VFE39_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259966.1"
                     /translation="MATSVVAPQRTSWAPVAALGLAMLVVTSEMTIAAVTLPRLGADL
                     EVSAAATAWVLLAYALPMAAVAIPAGRWADGADVRSVFALAMIGTAATSVLTALAPTF
                     WLVVVGRLLQGAAGALVVAAYMPIITASVRHGQRGRAIGFVITIMTVGSMAGVPLGGL
                     VAGAFSWREVFLMKIPLLVVVLWVGLRSIPRNPHRGIPRPGTALVCEALLLGGAITAV
                     LLAFEEVQGRPAVTVGLAVLAIGLAVWWSRLAASRPVLTLVRRRAFAVTLVSLLAMSF
                     TGGLIAFLLPYFVADVLGGGPDLTGVALLFFVGAMAPISAVAGAVADRCGTTVVALVG
                     SAIGVLGMLSMLTLDAGSGLVDMAWRLVVLGIGAALFNPAINAAMLAGAPPGSEGVAG
                     GVGMTVRTIAMTVGPSVSALAWTLAGGGVAGFRAGVVVITAAAAVGLLVLFVPVRAR"
     gene            10545..>11977
                     /locus_tag="VFE39_15115"
     CDS             10545..>11977
                     /locus_tag="VFE39_15115"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6259967.1"
                     /translation="MNGAASRRARSTWPPPVMHGRAAELDTVEALLDGARAGRSAALV
                     VRGEAGIGKSALLGHATAEAADMTVLAQTGVETESELPYAALHLLLKDVTEGADDLPE
                     AQAGALRAALGLAAAGPPPDRFLVGLAVLTLLAELACDRPVLCVLDDAHWFDRASAEV
                     LLFVARRLEAEAVVMIFAARTRHAPAFPAPGLAELSLPRLDDVAARCVLDAAADDLPR
                     HVREQLLREAAGNPLALHELPAAHRAGRAPVYPLGPPEAGRGGSAPTHSPVEREFAAR
                     IAALPERVRTLLLVAAADGTCDTGVVLAAAQRLGCSEADLEAAERARLLMFQEGCLGF
                     HHPLIRTAAYGSATMVRKQEAHRALADVLEGRPDVDRRNWHRAAASIGPDEDVAAALE
                     ESARGATDRGSNEASAAAYERAGSLSPDPQRRAWRLAAAAQAAADAGHRDWAAALAVE
                     AGKHARDQVHLARLAMIHARLADEVNRP"
BASE COUNT         1567 a         4258 c         4427 g         1725 t
ORIGIN      
        1 gcgtcccggg tggcctcgta gatcagcatc agggtgaggt cgcgcagagc cgcgcggaac
       61 gtggggttgt ccgtgcgcgc gtcgcgcagc tccgagagcc gggccttggc cagtgggtgg
      121 tcgacgacct gcacgtccat ggcgcagagg ctagccgccc cgcgcaggca tcagattccc
      181 gctcgaacgg cctggtgggg cggcggcttc gggacgagcg cgcagtccgt ccagctcgat
      241 ccagcctcac tgcgcgttcg cggctgcggc tgtgcgcgca tcgtaggcag cacgggcggc
      301 cgcgatctcg ttctggtgcg actctgtcca catgaccagc gcctgaatgg tctcgtgcag
      361 cgtgacgccg agggacgtga gctcgtagtc cacccgcggc ggaacgacgg cgtgcaccgt
      421 gcggtgcacc agcccgtcgc gctcgagctg gcgcagggtg accgtgagca tccgctggct
      481 cacgccgtcg atctcgcgcc ggagctccgt gaagcggagc ctgcgccggt cgagcagggc
      541 gatgaccagc agcgaccact tgtcccctat gcggtcgagg atctgccgga cctcgcagtc
      601 ctcacgggta tcccactggt aggcgtcggt gtagggcccg tcggtgccgc aggagtgcgt
      661 cggtgacttc gaagtgcctt cttccacgcc ttcagatggt gacgcacgat gaggtcggtt
      721 acaagaagga accgaccgaa tggagcaaat cggtgtccct atccccgtcc cccgcgctcg
      781 acgagcgcat gagcggtcgc gcgtgggggc tgcttctcgt cctctgcggc gcgatcttcc
      841 tggaggggat cgacgtctcg atgatgggcg tcgccctgcc atcgatccgc gccgagctgg
      901 gcatgtccac cagctcactg cagtgggtgg tgagcgccta cctgctcggt tacggcggtt
      961 tcgtcctgct gggcggccgg gccgccgacc tgttgggcag gcgccgcatg ttcctcacct
     1021 ggctcgtggt gttcgtggcc ttctccggcc tcggcgggct cgccaccgag ggctgggtcc
     1081 tcgtcgtcgc ccgcttcgtg accggtgtgg ccgccgcgtt catggcgccc gccgggctgt
     1141 cgatcatcac gacgaccttc gccgaggggc gcgcccgcaa ccgcgccgtt ctcgtctacg
     1201 ccggggcggc cgcgggcggc ttctcactcg gcatggtcgt cggcggcctg ctgaccgcca
     1261 tcgactggcg gtgggtgttc ttcgcgcccg tactggtggc cgcagtgatc ctcgtggccg
     1321 cggtgctgct cattccgcgc gagcagcggc ccagccggat ccgtatcggc gagttcgacg
     1381 ccgccggtac ggccggcctg acgggcgcga tgctgctgct cgtcgtcacc gtggtcaggg
     1441 cgcccgacgt cgagctcgga acgacgctcg gcacgctcgc cgccagcatc gcgctgttcg
     1501 tggcgttcgt gctgatcgaa cggcgctcgt cggcaccgct ggtccggttc gggatcctgc
     1561 gctcggcccc gctgctacgg gccaacgcag gcgcgctgct gctggtcggc tcgttcatgg
     1621 gcttccagtt cacggtcgtg ctgtacctgc aggagctgcg cggctggtcg gaggtcgaga
     1681 ccggcctcgc gctcgccgtc gcgggcatcg acgccgtgct cgcgccgacc ctcacgccgt
     1741 ggctggtgaa ccgcttcggc aacgccaggg tggtcgtcgg cgggctgctg ctctcggcgg
     1801 tcgcgtacct gctgttcctg ccggtcgggc tcgactggag cttcgttgcg atgctcccgg
     1861 cactgctcgt gctgggcgtc gcgttcgcgc tcgcctacgg cccactcacg atcgccgcca
     1921 ccaacggcat cgccgacgag gaacagggcc tcgcgggcgg gctgctgacc acgtccttcc
     1981 agttcggggc ggcgctcggg ctggcggtgg tcgcagcggt gatcgtggcc aacacggcgg
     2041 acgactcgcc gcaggccgtg ttcgacggct tccgggccgg cctggtcgtc ccgctcgtcg
     2101 cggcggtgct cggcgccgcc ctcacggcgt ccgggctgcg gcgcaggacc gctcccgcgc
     2161 gcacggcctg agctcgcgcg cggccccggg cccacggctc cactgccgca ccggttcgtc
     2221 ccagaaggcg tgaccgtgcg gccatcgggg ccgcgcaccc cgcggtgaac gagcgtctct
     2281 ctacgggttc acggcaccgg gcggcccggg gtctcgctcg tccagaccca ccggtcgtcc
     2341 ggttgccggg tggcgcgcac ccggtcctcc agcaccgggt cgagctcgtg ctcccggcag
     2401 ccggtggctg cccagcagga cagcacgatc tcgtcgcgcg tcgcgtccag ccgcaggaag
     2461 ctcttgaact gcgggacggc gttcgagtcg aacagcagcg acatccagtt gtgcgcgggt
     2521 gcggtgcctc gggcaggcaa cgggaagatg cggtcggcgg cgcggcgcgc tctctcggac
     2581 acggctggcg agccaggccg tggcggcgcg atgcccaccc gctccgccat cagcgtggtc
     2641 gcgtcgtccg gggagatcac gagcctgccc aacccgaagc cgaggagctt gtcgtagcgc
     2701 cggctgaagt gggcgagcga gtcgccgcga agcgggtagc agcgaaacgc ctcctcgtgg
     2761 acggcggggg cgagccggtc cacgttcggg atctggtggg tggccgagag ataggcaccc
     2821 gagccgcccg ccacgacgta ttggatcgtg cggccgtccg gctgcagcac ggggtagcgc
     2881 tggtagttgt ggtcgtcccc gccgatggct gcgacgtagt ggttcgccgg gtcggtgacg
     2941 atgtcgttca cgtccccgcc gccctcgatg gggcacgcct gcacgtgggc gtaggtgtag
     3001 atcgggcggc cggtgagaag gatcttgggc cggtcacccg cggacaccgc acagagccac
     3061 tcggcctggt cgctgtcgat gtgaccgtcc atgcccacgt cgatgagcac cagccgcacc
     3121 gggcctgcgt cgatggcgca gtacgggccc ggctgccctg cctgctgcga gtcggcgttg
     3181 cggaaccagc gcatgtcggc cacatcctcg gcggtggcct tcggcgcacg ccgccagagc
     3241 agctgtcgca acatccgctt ccacagcggg cctgccatgg cgggcgacgg cgccccggcg
     3301 tcgggcgggg cgtcgcagaa ggcggtcatg aagccggtga gcccgtcgta ccagtcgtgg
     3361 ttgccgggca gggcgtagag cggcgccggg tagccggcat agggccggta gaagcggtcg
     3421 gcgtaggcgc ggacaccgcc tgccgggtac acgacgtcgc tgcagacgat cccgaggtcg
     3481 gtatccgccg cgacgcgttc cagcaccggc acgacgacgt actgcgaggc gtcaccctcg
     3541 ccagggtcgc cgagcactgc caccgagatg cgatcgcggt cggcgaagcg tgcgacgagg
     3601 cggtcgcgcg gtggtgccgg caccccctcg gcgcgggcgt aggcgtcgag ccgacgcagc
     3661 cactcccgcc gctggtcgtc ggttgggtca ccgaaccagc gcgccagcac gtcgttccgg
     3721 gaggcgatca gcggaccggg acgcaaccag gagaaccggt tgtcgacggg cgcgagctgg
     3781 gcaccgatgc ccgggcggtg gcagccccag cctgcgccgc ccgtcgagcg ccgggacggc
     3841 ccgccggcag gttcggcccg gacggcgctc actccgagcc cgcaacgaag cgcacgacct
     3901 ccgccgccag cgccggcccc gcgtcctcct gcaggaagtg cccggcgccg gtgatcgtcg
     3961 ggtgctcccg gcccgccgtg cccggcatgg tgcggcgcag gatcggctcc atcgccgccg
     4021 tgatcgggtc accgtcgctg aacgcgcaga ggaacgggat ctcgctgcgc gacagcgtcg
     4081 cccaggccgc ccggttggcc tccgtggccg gatcgtcggg ccgcgtcggt acgagccccg
     4141 gcatcgcccg cgggcccgcc ttgtggctct cgtcagggaa gggggcgtcg tagccggcgc
     4201 ggacctccgg ggggaggcgg gtgcggcagc cggactgcac cagccgcgcg acgtccaggt
     4261 gctccgcctt ctccacggcc cggcggaact gccaccacac ctcgggcatg tcgtggtcgc
     4321 cggtgggcag gccggtgttc gcggccacca cggcggcaaa gcggtcgagg tgctcggcca
     4381 ccagccgcag gccgagcagg ccaccccagt cctgcccgac gagcgtgacg tcgcgcaggt
     4441 cgagcacatc gaacgcgagc gctcgcagcc attcgacgtg ccgggcgtag ctgtggtcgg
     4501 agatctcggc aggcttgtcc gagcggccga agccgaccag gtcgggggcc acggcccgca
     4561 gcccggccgc ggtcagcacc gggatcatcg tgcggtacag gaacgaccag ctcggctcgc
     4621 cgtgcagcag cagcacgacc ggcccgtccg ccggcccggc ctccacccac gccatacgca
     4681 gcgtgtggcc gtcgccgtca ggaacgtcgg cgtaacgggg atcgaggtcg aagccgggga
     4741 ggtcggcgaa ccgctcgtcg ggggtgcgaa gggtgcgcat cccgtgatct tgacagttca
     4801 cccggctccc gcgggagctc cgcgctgagc gatcttcggc ggcgtatccg gccgccggcg
     4861 cggggctcgg gagatgtcgc cgacggtgac gagcgcgact gcgtgcagag ggcacctcag
     4921 ggctctgcag ggcccggaag cgcgccgcgg ggcccgtcac cgcatcgagg ccgggctgcg
     4981 agcccgatcc ggcggtaccc gattaccttc cgcaatcatc cggatccacc tgccccgatc
     5041 gcgtgatggg ccaccgaggc cagatgagca gcgaaaacgc gcgctcgacg aacccctcga
     5101 cacccgccga cgcaggctga aacggcacca tcgacacgca cagtgataat gactaagcgt
     5161 gtcaagatcg acaaagttga tcctggtcga taccggagat ccaccgattt atgcgcctgt
     5221 cgcccggatg agtgcaccgt cagtggtcga acttgcagat tcaccctgac ccgaatgggt
     5281 ggtctgccac aaacactgtc acgcaggagg ccccgccgac gctgtactta tgactaggag
     5341 gtgatccggt gccggagaac agaatcaccg acgaagcgac gctggtcgct gcggcggtga
     5401 agctcacgca gtgcgacgga tacgtcgtgc tggccgtcga tcctcagacc ggggaggtcg
     5461 atgcacatgg gccgttcgac ggcctgacgg cgactctcaa ggccgaccag cttcgccgcg
     5521 atttcgaccg cggtgggctg gaggacgtga ccgtcggcgt ggtccgccta cacagctcca
     5581 cctgaaccca accacttcac cacgttcccc acaatcgcca tcgcccgctg cgccacggct
     5641 cggcgggcga tgtgctgtcc ggaggcggac gcgacgcgtt ccttgatcac cggtcgggcg
     5701 cgttctccgg ccacctccgg ccgattccgc gccggatcgc tgatcatgcc tgcgccgagg
     5761 cgcgagcgcc ccatgcgcgg tggggaacca atcgtgatcc cggctctcgt cgaacaggat
     5821 ctagcggaca cgccctagac tcaggcgccg tgaccacgac cgtccgcgcc ccgcggcccg
     5881 acgcgcgcag taccggcgga gcacgcctgg agacccgcgc cggcgcgggg ttcggcgacg
     5941 ccgtgcgcga cgaggcgtcg ttgcgccgtt tcctgcgcgg cctgccgggc gtcgaccagg
     6001 tcgggctcga gcgccggtcg gcgacgctgg ccagccgcag catcaagaag gagtcgaagc
     6061 tgcaggcgct ggacatggcc atctcgatga tcgacctcac gaccctggag ggtgctgaca
     6121 cccccggcaa ggtgcggtcg ctgtgcgcgc aggcccgccg tcccgatccc gagcaccccg
     6181 acgtgccgcc cgtcgccgcc gtctgtgtct accccgacct cgccggggtc gccgtggagg
     6241 cattgcgcgg gtcggcggtg gctgtggccg ccgtggccac cgcgttcccg tccgggcgct
     6301 cgtcgcgggc ggtgaagctc gccgacaccg cgttcgccgt ggatgccgga gcgtccgaga
     6361 tcgacatggt gatcgaccgc ggcgcgttcc tcacggggcg ctacggcgcc gtgttcgacg
     6421 agatcgtcgc ggtcaagcag gcctgtggca gcggcgagcg gcgggcacac ctcaaggtga
     6481 tcctcgagac cggcgagctg gccggctacg acgacgtgcg gcgggcgtcc tggctcgccc
     6541 tgctcgccgg cgcggacgtc atcaagacct ccaccggcaa gatctccccc gccgccaccc
     6601 tgccggtggt gcaggtgatg ctccaggccg tccgcgactg gcaccgagcc acgggggagc
     6661 tgcgtggcgt caaggccgcg ggcgggatcc gcaccgccaa ggacgcgatc cggcacctgg
     6721 tggccgtcaa cgaggtggcc ggaccggcgt ggctcacgcc ggagctgttc cggttcggcg
     6781 cgtcgtccct gctcggcgat ctgctccggc agcgccgcac ccagctcgac gggcactacg
     6841 agagcaccga ccggatcggg agcgcgtgat ggactggaca tacgcgcccg cccccgagtc
     6901 cgcctcgttg gccaacctgc agcccagcta ccggccgttc gtcgacgggg agttcgtcga
     6961 cggcggcggc cagccgctca agacgctcaa cccggcgacg gaagaagtgc tcgccgaggt
     7021 cggcacggcg agcacggagg acgtcgaccg ggccgtcgcc gccgcccacc gcgcgttcga
     7081 gaccacgtgg ggtccgatgc cgggcgcgga acgggccaag tacctgttcc ggatcgcgcg
     7141 ggtggtcgcc gagcgcgccc gcgagctcgc cgtactggag acgctggaca acggcaagcc
     7201 gatccgcgag tcccgcgacg tcgacgtgcc caccgcgtcg gcgcacctgt tccaccacgc
     7261 cggctgggcg gacaagctcg gctacgcggg gctgggcccc gacccacggc cgctcggcgt
     7321 ggtcggcgcc gtgatcccgt ggaacttccc gctgctcatg gccgcatgga agatcgcccc
     7381 cgcactggcg tgcggcaaca cgatcgtcct caagcctgcc gagaccacgc cgctcaccgc
     7441 gctggtgctg gccgagatcg cggccgaggc gggactccca cccggtgtgc tcaacgttct
     7501 tccgggcgcc ggcgacgtcg gggccgccct cgtgggccac gaggggctgg acaaggtggc
     7561 cttcaccggg tccaccgcag tcggcaagga gatccagcgc cgcctcgccg gcaccggccg
     7621 gcggctgacg ctggagctcg gcgggaaggc ggccaacatc gtctacgacg atgcgccgct
     7681 cgaccaggcc gtcgacggcg tcgtcgacgg gatcttcttc aaccagggcc acgtgtgctg
     7741 tgccgggtcg aggctgctgg tgcaggagtc gatcgcggac gagttcgagg cgctgctgcg
     7801 cgcccggatc gagacactgc gggtgggcga tccgctcgac aagaacaccg acgtcggcgc
     7861 catcaactcg cgcgcccagc tggaccgcat cgtcgacctc gcggcggcgg gcgacgccga
     7921 gggcgcgcgc cgctggacga gctcgtgccc gctgcccgag cgcggcctct tcttcccgcc
     7981 cacggtgttc tccggagtcg agcagacgat gcgggtggcg cgggaggaga tcttcgggcc
     8041 ggtgctgtcg gtgctcacct tccgcacgcc cgacgaggcc gtcgcgaagg ccaacaacac
     8101 cccgtacggc ctctccgccg ggatctggac ggagaagggc gccaaggcgc tgtggacggc
     8161 gcaacgcgtg cgcgccggcg tcgtctggac gaacaccttc aaccgcttcg acccgacggc
     8221 tcccttcggg gggtacggcg agtccggctt cggccgggaa ggcggccgga tcggactcga
     8281 ggcctacctc gacgcctgac ggcggcatgg cggtcggccc gaggcgcact tcaccgcctg
     8341 agcagggctg ccaggtacga gtcgcgacga tcatcgatgc cgctgcatga tcgagacgga
     8401 cgccggcgcc cccgccgggg ctgtcaggtg cgagtcgtca tgatcatgag ctctccgtcg
     8461 cgcgccggtc acgtgacgga ttcggcggca ccctccccgc tcctagcgtc gctttcacca
     8521 acagcgacga gggaaggagc ccaccatgaa cgatcgacca ctccgtctgg tggtgatcat
     8581 cggcagcacg cgggagggcc ggttcgcgcc gacggtggcc cgctggatca cgggggaggc
     8641 gcggcagcac gccgacctgg acgtcgacgt gctggatctc gccgacaccg cactgcccga
     8701 ccggctgacc ggtttcggcc agccgccacc gccggaggtc gcggcggtcg caccacgcct
     8761 ggcggccgcc gatgccttcg tggtggtcac gccggagtac aaccacagct atccggcacc
     8821 cctgaagaca ctcatcgact ggcacctggc ggagtggcag gccaagccgg tggggttcgt
     8881 gtcctacggc gggatgtcgg gcgggctgcg ggccgtggag cacctccgcg cggtgttcgc
     8941 cgaggtgcac gcgacgaccg tgcgcgagac cgtcagcttc cacggcgcct gggcccggtt
     9001 cggcgaggac gcccgcccgc tcgacgagct ggagtgctcg gcggcggcca agacgatgct
     9061 cgaccagctc gcctggtggg ccgatgccct gcgcaccgcc cgcacccggc ggccgtacaa
     9121 cgcggggtga gcgcgcttga cctcaacctc gcttcagctc ctacggtcgg tttccggagc
     9181 accggaggaa ggacgaccat ggccacgagc gtggtagcgc cgcagcgcac gagctgggcg
     9241 ccggtggcgg cgctgggtct ggcgatgctg gtggtcacgt cggagatgac gatcgccgcg
     9301 gtgaccctcc cccgtctcgg cgccgacctg gaggtctccg cggcggcgac ggcgtgggtg
     9361 ctgctggcct acgcactgcc gatggccgcg gtcgccatcc ccgcaggacg ctgggcagac
     9421 ggcgcggacg tccggtccgt cttcgccctg gcgatgatcg ggaccgcggc gacgagcgtc
     9481 ctgaccgctc tcgcgccgac gttctggctg gtcgtggtgg gcaggctgct gcagggggca
     9541 gcgggcgcgc tggtcgtggc cgcctacatg ccgatcatca cggcgagcgt gcggcacggg
     9601 cagcgcggac gggcgatcgg gttcgtcatc acgatcatga cggtcggcag catggcgggc
     9661 gtcccgctcg gtggcctcgt ggccggcgcg ttcagctggc gcgaggtctt cctgatgaag
     9721 atcccgctgc tcgtcgtggt gctgtgggtg ggcctgcgca gcatcccccg gaacccacac
     9781 cgcggcatcc cgcggcccgg cacagcactg gtgtgcgagg ccctgctgct cgggggcgcg
     9841 atcacggcgg tgctgctcgc gttcgaggag gtccaggggc ggcctgcggt caccgtcggg
     9901 ctggcggtgc tggcgatcgg cctcgcggtc tggtggtcgc ggctggccgc gtcccgaccg
     9961 gtgctcacgc tggtgcgcag gcgcgcgttc gcggtcacgc tggtctcgct gctggccatg
    10021 agcttcaccg gtgggctgat cgcgttcctc cttccctact tcgtcgccga cgtgctcggc
    10081 ggcggcccgg acctcaccgg cgtggcgctg ctgttcttcg tcggcgcgat ggcaccgatc
    10141 tccgcggtcg ccggtgcggt ggccgaccgc tgcggaacga cggtcgtggc gctggtcggg
    10201 agcgcgatcg gcgtgctcgg gatgctgtcg atgctgaccc tcgacgccgg gtccggcctc
    10261 gtcgacatgg cgtggcgact cgtggtgctg gggatcggcg ccgcgctgtt caacccggcc
    10321 atcaacgcgg cgatgctggc aggcgcgccg ccgggatcgg agggggtggc gggcggcgtc
    10381 ggcatgacgg tcaggacgat cgcgatgacc gtcgggccat cggtgtccgc cctcgcgtgg
    10441 acgctcgccg gcggtggggt cgcggggttc cgggcggggg tcgtggtgat cacggcagcg
    10501 gccgcggtcg ggctgctcgt gctgttcgtc ccggtgcgtg cgcgatgaac ggggcggcga
    10561 gcaggcgggc ccggtccacc tggccgccgc ccgtcatgca cggccgcgcc gcggagctgg
    10621 acacggtcga ggcgctgctc gacggcgccc gagccggacg aagcgccgcg ctggtcgtga
    10681 ggggggaggc ggggatcggc aagtccgcgc tgctcgggca cgcgacggcg gaggccgcgg
    10741 acatgaccgt gctggcccag accggcgtcg agacggagtc cgagctgccg tacgccgcat
    10801 tgcacctcct gctcaaggac gtcacggaag gcgccgacga cctgccggag gcgcaggccg
    10861 gcgcgctgcg ggcggccctc ggactcgccg cggcggggcc gccgccggac cggttcctcg
    10921 tcggcctcgc cgtgctgacg ctgctcgccg agctggcctg cgaccgtccg gtgctgtgtg
    10981 tgctcgacga cgcccactgg ttcgaccgag cgtcggcgga ggtgctgctc ttcgtcgcgc
    11041 gccggttgga ggccgaggcg gtggtgatga tcttcgcggc acgcacgcgg cacgcccccg
    11101 ccttcccggc acccggactg gcggagctca gcctgccacg gctggacgac gtggccgccc
    11161 gctgcgtgct ggacgccgcc gcggacgatc tgccgcggca cgtgcgcgaa cagctgctgc
    11221 gggaggccgc cggcaacccg ctcgccctgc acgagctgcc cgccgcccac cgcgccgggc
    11281 gcgcccccgt gtacccgctc gggccgcccg aggcagggcg cggcggctcg gcgccgacgc
    11341 acagcccggt ggagcgggag ttcgccgccc ggatcgcggc gctgccggag cgcgttcgca
    11401 ccctcctgct cgtggctgcg gcggacggca cgtgcgacac gggcgtggtg ctcgcggcgg
    11461 cgcagcgcct cggctgctcg gaggcggacc tggaggccgc cgagcgcgcc cggctgctca
    11521 tgttccagga gggatgtctc gggttccacc atccgctgat ccgtacggcc gcctacggca
    11581 gcgccaccat ggtgcgcaag caggaggcac accgagcgct ggccgacgtg ctcgagggac
    11641 gacccgacgt cgaccgccgc aactggcacc gggccgcggc gagcataggc cccgacgagg
    11701 acgtcgccgc cgccctcgag gagagcgcgc ggggcgccac cgaccggggc agcaacgagg
    11761 cgtcggccgc cgcctacgag cgcgccggat cgctcagccc cgacccgcaa cggcgggcct
    11821 ggcggctggc ggcggcagct caggccgcgg ccgatgccgg gcaccgcgac tgggcggcgg
    11881 cgttggcggt cgaggccggc aagcacgcga gggaccaggt gcacctcgcg cggctggcca
    11941 tgatccacgc acgcctcgcc gacgaggtca accgtcc
//
LOCUS       DASRSC010000306        10733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3072041,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000306 DASRSC010000000
VERSION     DASRSC010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10733
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3072041"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1398)
                     /locus_tag="VFE39_15120"
     CDS             complement(31..1398)
                     /locus_tag="VFE39_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HET6259968.1"
                     /translation="MSATTETPHKKKDRTHYLYIAVVVAVVLGVAVGLVSPELGKNLK
                     PLGTAFVNLIKMMISPIIFCTIVLGIGSIRKAATVGRVGGLALAYFLAMSTFALAIGL
                     IVGNILQPGAGLQLGGLKPYTAPAAESMSDFVVGIIPTTLFSPLVGDSVLSTLFVALL
                     VGFAVQALGRSGQPILRGVKHVERLVFRVLAMVMWAAPIGAFGAIAAVVGETGWSAVI
                     ALFQMMAAFYLTCALFVFGVLGPVLRLGAGISIFSLLRYLTREFVLIVSTSSSESALP
                     RLIAKMEHVGISRPVVGITVPTGYSFNLDGTAIYLTMASIFIADALGRPMAIGEQIAL
                     LLFMIIASKGAAGVSGAGLATLAGGLASHRPDLVDGVGLIVGIDRFMSEARAVTNFAG
                     NAVATVLIGNWVGEFDRERAARVLSGAEPFDETTMLDDHEQAEPEETTPAAVPAPRKP
                     EVLTS"
     gene            1535..3124
                     /locus_tag="VFE39_15125"
     CDS             1535..3124
                     /locus_tag="VFE39_15125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005448918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET6259969.1"
                     /translation="MAGQRSRWSLAGQLFALQAAIIVIVLTGVGVATYFQFDETNRKA
                     TEEEMLGIAHTLAASPEVRDALDAPKPSAVLQPLAERVRMDTATDFVVVMTVDGTRFS
                     HPNPAEIGRTFQGTIGPAARGNQHFTETYAGTLGPSVRAVVPVLDGGNVVALVSVGRT
                     VAAVSRELEQQMPLFLGAATVALLLAAAGSWLVSRWLRRSTHDLGPAELARMYEYYDA
                     VLHAVREGLLLLDRSGRVQLVNDEAQRLLVLPPDVQGRRVDELGLPAELGAALATGEA
                     RDDEIHLTADRIVVVSQGAARWGGRHLGTVVTLRDHTELRALVSELQTIRGFADSLNA
                     QAHEAANQLHTVISLIELGRSDAALEFAATELAVSQQLTDTVLAGISVPELAALVVGK
                     AAEAGERGVTLHVEEGTCVPAGIADPRDLVTIVGNLLDNAIDAARDAPEPRWVRIAAV
                     VTDDAVELRVADSGPGLAAADAERAFRRGWTTKPHDGLGRGLGLALVRQAAERHGGQV
                     ELGRVGAGGAEFAVRLPLVRT"
     gene            3121..3828
                     /locus_tag="VFE39_15130"
     CDS             3121..3828
                     /locus_tag="VFE39_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HET6259970.1"
                     /translation="MIRTLIVEDDPIAAAAHASYVGRVRGFEVVGSAGSGTEALRMLA
                     DRTIDLVLLDVNLPDTSGLEVLRRLRAAGHTTDVIMVTRARDLAVVQAAISFGATQYL
                     VKPFTFSAVRAKLEVYLDYRSRLGAGPVVVQEDVDDLFGTLRAPVESSGLPKGVSRES
                     LDAVVRTLTDAGAAGLTAVEVADAVGASRVTARRYLEYLAETGLAERHVRYGGAGRPQ
                     VEYRWRATGVPGGRAAH"
     gene            complement(3853..5622)
                     /locus_tag="VFE39_15135"
     CDS             complement(3853..5622)
                     /locus_tag="VFE39_15135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET6259971.1"
                     /translation="MPAAPPTGKPPLARPPTARTPSARTSAGTTAAKATPTTKSDTTK
                     IPAAKIPAATTPATKNPAAKVPPARTRPAGGGTASGATGHRASARGASARRAAPGRPP
                     RTLGRALLATAAATVAPGSGHLMLRRQRTGALILGVFLLTLVALGALGLSARKTALLE
                     NLLSTHVLTIIAIGCVVAALAWIAVIVRTYLLARPARLGASRQALGVGVVVALCLVVA
                     APLGFAANLANSQRNLLDSLFTGGGGTAVAEAITKPRLNILLVGSDAGPDRTGTRTDT
                     MMVASIDTKTSRTTLFALPRNIGHAQFPPGSPMAKKFPNGFYDKADPMSGNYLLNAVY
                     AYGHEYPAVAPSGPTADPGMNLLHQTVAYMLGLQLDYYVEVNMAGFASIIDAIGGITV
                     DVGPTPLPIGGVLPDGTHVKPDGYVPAGVQTLNGDQALWFARSRRNSDDYSRMGRQRC
                     LLNSVLQQKSPTDVLTNFQAIASATTNSVSTNIPQQVLPALVSLAGGGNVVLESVSFD
                     PNLPDPNAADGKFDTLSPDFELMREVVQNSINKPPPPPPPPSAAAPTTTSKSTKSKAS
                     SSPTTTSAAPDSSQPQSLAESCG"
     gene            6196..6924
                     /locus_tag="VFE39_15140"
     CDS             6196..6924
                     /locus_tag="VFE39_15140"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HET6259972.1"
                     /translation="MSDQRSSVAAVVLAGGSGTRVGGERNKVYLPIAGRSVLSVSLNT
                     FSAVPEIGPVVLVVRPEDLDLAAEVLDREVEHAVEVVTGGATRQESELAGLRQLAGRI
                     HDGTVGTVLIHDGARPFVGRPLVQKVLRVAQESGGAVPGLPRDDLATAGPDGSGLAGP
                     APDGLVAVQTPQGFWAAPLLAAYEEAAREGFAGTDTASCMQRFAPLVRIRWVEGEQRN
                     FKITYAHDLVVAREVPAPLGAEQM"
     gene            7010..7486
                     /locus_tag="VFE39_15145"
     CDS             7010..7486
                     /locus_tag="VFE39_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6259973.1"
                     /translation="MRFDGAQLSVEVLGPRLRVIRMAGVLDPVTVTRLAAVTEAQMAR
                     CAGHIVIDLGNVRYFGTDDLDVLVTVRDAASAAGVRVHLAGLAAREALLPAGIIGALG
                     QFSTFHTVEQAERELVGQLAAVVTGGAPRGRYARSGPPRTTDRGRGARPGGPLRSA"
     gene            7565..7846
                     /locus_tag="VFE39_15150"
     CDS             7565..7846
                     /locus_tag="VFE39_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259974.1"
                     /translation="MLSRAEADLAHIVDADRAGATRRLAASAARVIMASGESGDRRRA
                     WLRRLLADLLGTDCWIDAVPVIAQLNRAAFDALSAWLCHRLSTEADDGT"
     gene            8506..8727
                     /locus_tag="VFE39_15155"
     CDS             8506..8727
                     /locus_tag="VFE39_15155"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HET6259975.1"
                     /translation="MIEAVELIDAIRDLPEEQKAVIMHLYFRASSLLDASSALDVPKG
                     TVKSRAHYALRAFRKSISESADTEGRAAE"
     gene            8742..9317
                     /locus_tag="VFE39_15160"
     CDS             8742..9317
                     /locus_tag="VFE39_15160"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HET6259976.1"
                     /translation="MNMDHGMNMHHDIEQLVALAVGLSDASDRSAVEAHLAGCPACRQ
                     ELRELREIDAAIRRVPPEAFLEGPPADADLVLQRVLREVRTQPRVRSAAEAASSVRSC
                     GADARTGPELVAAVAEAGDGVRVTAVVVGVPAGEPCTLVVIDRAGGRHSAVRWIAPEC
                     GAAGVQGSASVRIDDVVAVAVVAADGSGIIS"
     gene            9963..>10733
                     /locus_tag="VFE39_15165"
     CDS             9963..>10733
                     /locus_tag="VFE39_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HET6259977.1"
                     /translation="MTDQRERLATYRAKRDFAATPEPAGSEPAAGSGRFVVQRHRARR
                     LHYDLRLEVDGVLASWAVPKGPTLDPKARQMAVHVEDHPIEYFDFEGIIPRGEYGGGD
                     VIVWDWGTWEPSGTEDPLQAIEDGELHFDLHGEKLAGRFVLVRREDGRPGKEQWLLLH
                     KNDEHARQGWDPEEHPQSVKSGRTNDEVAAAPDTLWRSDLPAGEAAVRLARPTLPSWD
                     PPTEDELRALDAIRRQGRWEVQGLEVRVTNLDKVLFPAR"
BASE COUNT         1559 a         3568 c         4056 g         1550 t
ORIGIN      
        1 cgacaacacg cgggcacccc cccaactttg ctaactggtg agcacttccg gcttccgcgg
       61 ggcgggcacg gcggcaggcg tcgtctcctc cggctcggcc tgctcgtggt cgtcgagcat
      121 ggtcgtctcg tcgaacggct cggcaccgct cagcacgcgg gcggcgcgct cccggtcgaa
      181 ctcaccgacc cagttgccga tcaggaccgt ggccaccgcg ttgccggcga agttggtgac
      241 ggcgcgggcc tccgacatga accggtcgat acccacgatg agaccgacgc cgtcgacgag
      301 gtcggggcgg tgcgaggcca ggcctcccgc gagcgtggcg agcccggcgc cggacacccc
      361 tgctgcaccc ttggaagcga tgatcatgaa cagcagcagg gcgatctgct cgccgatcgc
      421 catcggcctg ccgagcgcgt cggcgatgaa gatcgacgcc atcgtcaggt agatcgcggt
      481 gccgtcgagg ttgaacgagt agccggtggg aaccgtgatg ccgacgacgg gacgggagat
      541 gccgacgtgc tccatcttgg cgatcagccg cggcagcgcc gactccgacg acgacgtgga
      601 gacgatgagc acgaactccc gggtcaggta gcgcagcagc gagaagatgc tgatcccggc
      661 gccgagccgc agcaccggcc cgagcacgcc gaacacgaag agcgcgcagg tcaggtaaaa
      721 ggctgccatc atctggaaca gcgcgatgac cgccgaccag ccggtctcgc cgacgaccgc
      781 cgcgatggca ccgaaggcgc cgatcggggc cgcccacatg accatcgcga gcacccggaa
      841 gacgagccgc tcgacgtgct tcacgccccg caggatcggc tggcccgagc gcccgagggc
      901 ctgcaccgcg aagccgacca gcagcgcgac gaacagcgtc gagagcacgc tgtcgccgac
      961 cagcggcgag aacagcgtgg tcgggatgat gcccaccacg aagtccgaca tcgactcggc
     1021 cgcgggtgcg gtgtacggct tcagaccgcc cagctgcagc cccgccccgg gctggaggat
     1081 gttcccgacg atgagaccga tcgccagcgc gaaggtggac atggcgagga agtaggccag
     1141 cgccaacccg ccgaccctgc ccaccgttgc ggccttccgg atggacccga tgcccaggac
     1201 gatcgtgcag aagatgatcg gcgagatcat catcttgatg aggttcacga aggccgtgcc
     1261 gagcggcttg agattcttcc ccagctcggg cgagaccagg ccgacggcca cacccaatac
     1321 caccgcgacg acgacggcga tgtacaggta gtgggtcctg tccttcttct tgtgcggtgt
     1381 ttcggtggta gctgacacgg gtcctccggt aggccgacga cgacggcgga cgttgcgcgg
     1441 ataccggtgc accggcaacc atcgcggcga gcatggaggg cgttgtggtg gcagtcacgg
     1501 tttcgttcat ttcgttcgac gggcggtgac gacagtggcc gggcaacgga gccggtggag
     1561 cctcgccgga cagctgttcg cgctccaggc ggcaatcatc gtgatcgtcc tgaccggcgt
     1621 cggggtggct acctacttcc agttcgacga gacgaaccgc aaagccaccg aggaggagat
     1681 gctcggcatc gcgcacaccc tcgccgcgtc gcccgaggtg cgggacgcgc tcgacgcccc
     1741 gaagccctcg gccgtgctgc agccgctggc ggagcgggtc cgcatggaca cggcgaccga
     1801 cttcgtcgtg gtcatgacgg tggacggcac gcgcttctcg caccccaacc ccgcggagat
     1861 cggccggacc ttccagggca cgatcgggcc ggccgcgcgc gggaaccagc atttcaccga
     1921 gacctacgcg ggcaccctcg gcccgtcggt gcgcgcggtg gtcccggttc tggacggcgg
     1981 gaacgtcgtg gcgctggtgt ctgtcgggcg gacggtggcg gcggtgtcgc gggagctgga
     2041 gcagcagatg ccgctgttcc tcggcgccgc caccgtcgcg ctgttgctgg ccgccgcagg
     2101 gtcctggctg gtcagccgct ggctgcgacg cagcacgcac gatctcggcc cggccgagtt
     2161 ggcgcgcatg tacgagtact acgacgcggt gctgcacgcc gtacgggagg gcctgctgct
     2221 gctcgaccgt tcggggcggg tgcagctggt caacgacgag gcccagcggc tgctggtgct
     2281 gccgcccgac gtgcagggcc gccgtgtcga cgagctcggc ctgccggccg agctgggggc
     2341 cgcactcgcc accggcgagg cgcgcgacga cgagatccac ctgaccgccg accgcatcgt
     2401 ggtggtcagc cagggtgccg cgcggtgggg cggccggcac ctgggcaccg tcgtgacgct
     2461 gcgcgaccac acggagctgc gcgctctggt cagcgagctg cagacgatcc gcggcttcgc
     2521 cgactcgctg aacgcgcagg cccacgaggc ggccaaccaa ctgcacacgg tcatctcgct
     2581 gatcgaactg gggcggtcgg acgccgcgct ggagttcgcg gccaccgagc tcgccgtgtc
     2641 ccagcagctc acggacaccg tgctcgccgg gatctccgta cccgagctgg cggcgctcgt
     2701 cgtgggcaag gcagccgagg cgggggagcg cggcgtcacg ctgcacgtgg aggagggcac
     2761 gtgcgtgccc gccggcatcg ccgatccccg cgacctggtc acgatcgtcg gcaacctgct
     2821 cgacaacgcg atcgacgccg cccgggacgc gccggagcca cgctgggtcc ggatcgccgc
     2881 cgtcgtcacc gacgacgcgg tggagctgcg ggtggccgac agcggtcccg ggctcgccgc
     2941 ggccgacgcc gagcgggcct tccggcgcgg gtggaccacc aagccgcacg acgggctcgg
     3001 ccgcggtctc gggctggcac tcgtgcgcca ggccgccgag cgccacggcg gacaggtgga
     3061 gctggggcgg gtcggtgcgg ggggtgccga gttcgcggtg cgcctcccac tggtacggac
     3121 atgatccgca ccctgatcgt cgaggacgac ccgatcgctg ccgccgccca cgccagctac
     3181 gtcggccggg tgcgcggctt cgaggtggtc ggcagtgcgg gctcgggcac ggaggcgttg
     3241 cgcatgctcg ccgaccggac catcgacctc gtcctgctcg acgtcaacct gcccgacacc
     3301 agcgggctgg aggtgctgcg ccggctgcgt gccgcgggcc acaccaccga cgtgatcatg
     3361 gtgacgcgcg cgcgggacct ggccgtcgtg caggccgcga tctccttcgg tgccacgcag
     3421 tacctggtca agccgtttac cttcagcgct gttcgcgcga agctcgaggt ttatctggac
     3481 taccggtcgc ggctcggcgc cgggccggtg gtcgtgcagg aggacgtcga cgatctgttc
     3541 ggcacgctgc gggccccggt cgagtcgagc ggcctgccga agggtgtcag ccgcgagtcc
     3601 ctcgacgccg tcgtccgcac actgaccgac gcgggggccg ccgggctcac ggccgtggag
     3661 gtcgccgatg cggtcggggc gtcccgggtg acggcccggc gttacctcga gtacctggcc
     3721 gagacggggc tcgccgagcg gcacgtgcgc tacggcggcg ccggcaggcc gcaggtggag
     3781 taccgctggc gggccaccgg cgtcccgggt gggcgagcag cgcactgacc cgcgccgccg
     3841 ccctccggca gctcagccgc aggactcggc gagggattgc ggctgcgagg agtccggagc
     3901 cgcggaggtg gtggtcggac tgctgctcgc tttgctcttg gtcgacttgc tcgtggtcgt
     3961 cggggcggcg gcgctcggag ggggcggcgg cggtggcggc ttgttgatcg agttctgcac
     4021 gacctcacgc atgagctcga agtccggaga gagggtgtcg aacttgccgt cggcggcgtt
     4081 cgggtccgga aggttcgggt cgaacgagac gctctccaac accacgttgc ccccgcccgc
     4141 gagcgacacc agtgcgggca gcacctgctg cgggatgttg gtggacacgc tgttggtggt
     4201 ggctgaggcg atggcttgaa agttcgtgag cacgtcggtc gggctcttct gctggaggac
     4261 gctgttgagc aagcaccgtt ggcggcccat ccggctgtag tcgtcggagt tgcgccgcga
     4321 ccgggcgaac cacagtgcct ggtcaccgtt gagggtctgc acgccggccg gcacgtagcc
     4381 gtcgggtttg acgtgggtac cgtcgggcag aacgccaccg atcggcagtg gggtcgggcc
     4441 gacgtcgaca gtgatcccgc cgatagcgtc gatgatcgac gcgaagccgg ccatgttgac
     4501 ctcgacgtag tagtccagct gcagcccgag catgtaggcg acggtctggt gcagcaggtt
     4561 catcccgggg tcggcggtgg gaccggacgg cgcgacggcc gggtactcgt gcccgtaggc
     4621 gtacacggcg ttgagcaggt agttgcccga catgggatcg gccttgtcgt agaagccgtt
     4681 ggggaacttc ttcgccatcg gtgatcccgg cgggaactgc gcgtggccga tgttgcgggg
     4741 cagtgcgaag agcgtggtgc ggctcgtctt cgtgtcgatg ctcgcgacca tcatcgtgtc
     4801 ggtgcgggtg ccggtgcggt ccgggccggc gtcgctcccg accagcagga tgttgagccg
     4861 cggcttggtg atggcctcgg ccacagctgt gccgccaccg ccggtgaaca gcgagtcgag
     4921 caggttgcgc tgcgagttgg cgaggttcgc cgcgaacccg agcggggcgg cgaccaccag
     4981 gcagagcgct acgaccacgc cgacgccgag cgcctgtcgg gacgcgccaa gccgtgccgg
     5041 gcgggccagc aggtaggtgc ggacgatgac ggcgatccag gcgagcgcgg ctaccacgca
     5101 gccgatggcg atgatcgtca gtacgtgcgt ggacagcagg ttctcgagga gcgccgtctt
     5161 gcgtgcgctg agcccgagcg ctccgagggc gacgagggtc agcaggaaca cgccgaggat
     5221 cagggcaccg gtgcgctgcc tgcggagcat gaggtggccc gaccccgggg caacggtggc
     5281 tgcggccgtg gcgagcagag cgcggccgag tgtgcggggc gggcggccgg gggcagcgcg
     5341 acgggcgctt gcaccgcgcg ccgaggcgcg gtgacccgta gcgcccgagg cggtgccacc
     5401 gccggcgggg cgggttcgtg ccgggggaac cttcgccgcg gggttcttcg tcgcaggggt
     5461 ggtggctgcg gggatcttcg ctgcggggat cttcgtggtg tcggacttcg tggtgggtgt
     5521 cgctttggcg gccgtcgtcc cggcggaggt ccgcgcggac ggggttctcg ccgtgggggg
     5581 cctcgccagc gggggcttgc ccgtcggggg agcggcaggc atgcgctggt cgggtgcttg
     5641 tcgtggcaca ggccgcgtcg gcggctcggg ctcgcgtggc cgctgcggac gccgtggcgg
     5701 catgggacgg gcaggaacat ccgccgggcg cgccacccgt cggggcgggt cgggctgccg
     5761 gggcggaatc cctgcctgca gttcgcgctc gctgcgccgg ggccacggtt gctgtggggg
     5821 caatgggcgt cctccgcccc cgagggggcg ccggccgtca tcgatcaccg tgtccacccc
     5881 ctgcattcga ctgaatggag tacaaccacg actcggcggt gagcctgccg gagcctactg
     5941 cgagtgatga gcgcctcggc gatgcacctc gatcggatgg aataaggcca gctcgtacct
     6001 cgttctcgcg tggctttcga ggtgctcccg caacacagca ggcgccgagc gcacggctcg
     6061 gctgcaagcc ggtgtcggct gtctgccgcg acgggtacat ccgccgacga tactgagcga
     6121 acccggaccg cccatatccc acctcgcggc ggcggtggct tgaccccact gacctctccg
     6181 cgctgcactg gcgcggtgtc cgatcagcgc agcagcgtcg cggccgtcgt actggccggg
     6241 ggcagcggca cccgggtcgg cggtgagcgc aacaaggtgt acttgcccat cgccggacgg
     6301 agcgtgctct cggtctcgct gaacacgttc tcggcggtgc ccgagatcgg tccggtcgtg
     6361 ctcgtcgtgc gtccggagga cctcgacctc gccgcggagg tgctcgaccg cgaggtggaa
     6421 cacgccgtgg aggtcgtgac gggaggcgcg acgcgccagg agtccgagct cgccggcctc
     6481 cggcaactcg ccggccgtat ccacgacggg accgtcggca cggtgctcat ccacgacggc
     6541 gcgcggccgt tcgtcggccg cccgctcgtc cagaaggtac tgcgggtggc gcaggagagc
     6601 ggcggtgccg tgccggggct gccccgcgac gacctcgcga cggccgggcc cgacggcagc
     6661 gggctcgcgg ggccggcacc ggatggcctg gtggcggtac agacgccgca ggggttctgg
     6721 gctgcgccgt tgctcgcggc gtacgaggag gctgcgcggg agggcttcgc cggtaccgac
     6781 acggcgtcct gcatgcagcg cttcgcgccg ctggtgcgca ttcgctgggt cgagggggag
     6841 cagcgcaatt tcaagatcac ctatgcgcac gacctcgtcg ttgcacggga ggtgcctgcc
     6901 ccgctcggcg ccgagcagat gtgaccgtgg tcgtacccgc cgcgcggttg ttcgcgttgc
     6961 aaacgggtac tcggtgcctg gcgggatggc aggacgcggg aggtgccggg tgcgtttcga
     7021 cggagcgcag ctgtcggtgg aggttctggg gccgcggctc cgggtgatcc ggatggcggg
     7081 tgttctcgac ccggtgaccg tgaccagact ggctgccgtg accgaagcgc agatggcgcg
     7141 ctgtgcgggc cacatcgtga tcgacctcgg caacgtgcgt tacttcggca ccgatgacct
     7201 cgacgtgctg gtcacggtcc gggacgcggc aagcgccgcc ggggtccgcg tccacctcgc
     7261 cgggctcgcc gcgcgggagg cgttgctgcc ggcaggcatc atcggtgcgc tcggacagtt
     7321 ctcgacgttc cacaccgtgg aacaggccga acgggagctc gtcggccagc tggccgctgt
     7381 ggtcaccgga ggcgctccgc gcggtcgcta cgcccgttcc ggcccgccgc gcaccaccga
     7441 caggggccgc ggcgcccggc ccggagggcc gctccggagc gcctgacgcc ggacgatccg
     7501 gcggatctcc cggtatcgcg gatctgccgg tatctcagtg ccgggcgagc gtccgcgagg
     7561 tgcgttgctg agccgcgccg aggccgacct cgcccacatc gtggacgccg atcgggcggg
     7621 ggcgacgaga cggctggccg cgtccgccgc ccgggtcatc atggcgagcg gtgagagcgg
     7681 ggaccgccgg cgcgcgtggc ttcgccgtct gctcgccgac ctgctcggca cggactgctg
     7741 gatcgacgcc gtgccggtga tcgcacagct gaatcgcgct gcgttcgacg cgttgtcggc
     7801 gtggctatgt catcgcctga gcaccgaggc cgacgacggg acgtgaagga atcaggattt
     7861 gtccgaactt cctcggtgat ccgtcgaacc tcgcggtcct cccgtccgtg taggtagtag
     7921 aggaaccgaa ccacgccgag aggaggcgtc atgtccagct ccatccccgt cttctaccgc
     7981 aggaagttgg tcaagcgctc cgaagggggt gtctgattcg gatgtccgcg ctcagtgacc
     8041 cggccgaacg ccgccgccga aggatgcaac gacctcgcat gaggatgggc tctcagccgt
     8101 gacaggcgga tcgccatcct agatcgggtc gtgcagcccg ataacggata gcgatcaagc
     8161 tggttcgaac ggtaatgcgg gtgttggtac ggccgacgac gctgagcctg ccggatgtcc
     8221 ggctcgcgac gaggcccttg ttcgagagct gtacgagaaa catggagcgg cggtcctggc
     8281 gtacgcggaa cggctcaccg cgacagaggt gctgctgagg acgtgctgca ggagacgatg
     8341 ctgcgagcat ggcgtcaccc gtcaacggca agggctcggt gagggggtgc tgatgaccgt
     8401 ggcacaacgc atcgtgatca tcgcgcgcgt gccctgtcgg cccgtccgac cgagcagcag
     8461 gaggacgtcg gtgccgcgcc atcccagcgg gaccactccg accgcgtgat cgaggcggtg
     8521 gagctgatcg acgcgatcag ggacctgccc gaggagcaaa aggcggtgat catgcacctg
     8581 tacttccgcg cctcgagcct gctcgatgcc tcctcggcac tggacgtgcc gaagggcaca
     8641 gtcaagtcgc gcgctcacta cgcgttgcgt gcgttccgca agtccatcag cgaatcggcg
     8701 gataccgagg ggagggctgc cgaatgaggg aggacggcga catgaacatg gatcacggca
     8761 tgaacatgca tcacgacatc gagcagctcg tcgcgctcgc ggtgggcctc tcggatgcat
     8821 cggaccgctc ggccgtcgag gcgcacctgg ccggatgtcc ggcctgccgg caggagctcc
     8881 gggagctgcg ggagatcgac gccgccatcc ggcgcgtccc gcccgaggcg ttcctcgagg
     8941 gaccgcccgc ggacgccgac ctcgtcctgc agcgcgtgct gcgcgaggtc cggacgcagc
     9001 cgcgcgtgcg gtcggccgcg gaagcagcgt cgagcgtgcg gagctgcggt gccgacgcgc
     9061 ggacgggacc ggagctcgtt gcggctgtcg cggaggcggg cgacggcgtc cgcgtgacgg
     9121 ccgtcgtcgt gggggtgccc gccggagagc cgtgcacgct cgtggtcatc gaccgcgccg
     9181 gcggccgcca cagcgcggtc cggtggatcg caccggaatg cggtgccgcg ggcgtccagg
     9241 gatcggcttc ggtgcggatc gacgacgtgg tcgccgtggc agtggtggct gccgacggct
     9301 ccggcatcat ctcgtgatca tctgatacac gctcgacgag cgagggagac cggaatggcg
     9361 tcggcgttgg cggaggtcgt cgcgaacctg cgagccgagc tcaacgaggc catgcaggcc
     9421 agcgcggacg aggacctgct cttcgagctc gggaccgtgg agctgcagct gaccgtcggt
     9481 gtcgacaagg agaccaagcc ggggcgaagg tcaagttttg ggtgctcgag gtgggtgccg
     9541 agacgacgct cggatcgagc tcgacccagc ggatcaagct gacactcgat ccgcggcacc
     9601 ccgccactgc cgtgcatcga ggtccggtcc gtcccggggc gtggtggtcg cccgcgaggg
     9661 ttggaccgat ggcgcggtga cggcaggagc ggcgatgacc ccaccagcag cggcaccggc
     9721 gggacaggtg aacggcgccg aggtggaccg ggccgacttc atgcggcttg cccgatccct
     9781 tccggcgccc gccgaggacg ccatcgccga accggctgag ccgcaccagc gcgtgctgct
     9841 cgagcgcaac gtggcggcct tcgagaacgt ggacatgccc gcgctgacgc gggtgctgac
     9901 cgacgacgcc accacggcga acgccgggag tcgggaccgg ccccacgtgg taccccgccc
     9961 gcatgaccga ccagcgcgaa cggctcgcga cctaccgcgc caagcgcgac ttcgcggcca
    10021 cgcccgagcc cgccgggtcc gagcccgcgg ccggttcggg caggttcgtc gtgcagcggc
    10081 accgcgcccg acggctgcac tacgacctcc ggctggaggt cgacggcgtg ctggccagct
    10141 gggccgtgcc caaggggccc acgctcgacc cgaaggcacg ccagatggcc gtgcacgtcg
    10201 aggaccaccc gatcgagtac ttcgacttcg agggcatcat cccgcgcggc gagtacggcg
    10261 ggggggacgt gatcgtgtgg gactggggta cgtgggagcc gtccggcacc gaggatcctc
    10321 tgcaggccat cgaggacggt gagctgcact tcgacctgca cggggagaag ctggccggac
    10381 gcttcgtgct cgtccgcagg gaggacggca ggccgggcaa ggagcagtgg ctcctgctcc
    10441 acaagaacga cgagcacgcg cggcagggct gggatcccga ggagcacccg cagtcggtca
    10501 agagcgggcg tacgaacgac gaggtggcgg ccgcccccga cacgctctgg cgcagcgacc
    10561 tgcccgcggg ggaggcggcc gtccggctgg cccgtccgac gcttccgtcg tgggacccgc
    10621 cgacggagga cgagctccgg gcgctcgacg cgatccggcg ccagggccgg tgggaggtcc
    10681 agggccttga ggtccgggtg accaacctgg acaaggtgct gttccccgcg cgg
//
LOCUS       DASRSC010000307         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1762099,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000307 DASRSC010000000
VERSION     DASRSC010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762099"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /gene="lipB"
                     /locus_tag="VFE39_15170"
     CDS             complement(<1..437)
                     /gene="lipB"
                     /locus_tag="VFE39_15170"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HET6259978.1"
                     /translation="MQRTVHGRRVSGEVPDSCLLLEHMPVYTAGKRTAVSDRPFGDPG
                     APVIDVDRGGKITWHGPGQLTAYPIIKLREPVDVVAYVRALEESMIRVCAEFGLQAVR
                     VEGRSGAWIPASPARGLPERKVGAIGARVARGVTMHGLALNCD"
     gene            567..1457
                     /locus_tag="VFE39_15175"
     CDS             567..1457
                     /locus_tag="VFE39_15175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259979.1"
                     /translation="MINEAELREMTPSERRKLARSLTTVDYPHPLLGIYLARGRRLGA
                     LVSIIACTVLVGWIIVLVLTLHDSFHAQHWTGAWVGFDVILLFAFAATGWAFWRGRQI
                     VIACLIATGTLLCCDAWFDVVLDAGTSDVWLSVASAVVVELPLAFLMFNAARRLIRLS
                     AVVAVSEAGDSSDCDHLPEDELPPLWKIPLFGVGRRPAARASQTRAIETRPSETRPIE
                     TRATETRAPRTRSDEYHGASGRPAPITDLASQPWLPTFPRKRRRTRSSSPSRPTPTRG
                     SARSLPHWSSTCTRSPATSN"
     gene            1361..2194
                     /locus_tag="VFE39_15180"
     CDS             1361..2194
                     /locus_tag="VFE39_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HET6259980.1"
                     /translation="MESFAPDTDPRLREILASLVKHLHAFARDVELTIPEWEKAVDFL
                     TRTGHKSDDERQEFILLSDVLGLSMLVETISNRKFGVATESTVLGPFHVVQSPVRALG
                     DDIDLVKDPHATPCVVTGRVVSTDGTPLPGASIDVWQANDQGFYDVQQPDVQPRTNGR
                     GLFTADENGGFWFRTIVPSYYPIPTDGPVGEVLLATGRHAFRPAHIHFIVSAPGYRDL
                     TTHIFVAGSEYIESDTVFAVKKSLVVDFEEVDDPALAETWRVKVPFRHADFDIVMQPS
                     A"
     gene            complement(2227..>2758)
                     /locus_tag="VFE39_15185"
     CDS             complement(2227..>2758)
                     /locus_tag="VFE39_15185"
                     /inference="COORDINATES: protein motif:HMM:NF019938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HET6259981.1"
                     /translation="STPAGKAFLSRVVHDWEAAADPARAAGIRVAHVRSGLVLGAGGG
                     VLGRLAPPARFGLLPRFGNGYQVMSWISLTDEIRAIRFLLDGPGADERTGPYNLTAPN
                     AVTNGELTAALHAAFRRPDFPWLRVPAPLLKLALGEMSSELLTSARVLPARLLEAGFE
                     FSYPTIGAALAAELPR"
BASE COUNT          438 a          986 c          920 g          414 t
ORIGIN      
        1 cagtcgcagt tgagcgcgag cccgtgcatg gtgaccccgc gcgcgacccg ggcgccgatc
       61 gccccgacct tccgctcggg gagaccgcgg gccggcgagg cggggatcca cgccccggag
      121 cgcccctcga cccggacggc ctgcagcccg aactcggcgc agacccggat catcgactcc
      181 tcaagcgccc gcacgtaggc gaccacgtcg accggttcgc gcagcttgat gatcgggtac
      241 gcggtgagct gccccggccc gtgccaggtg atcttgccgc ccctgtccac gtcgatgacg
      301 ggcgcccccg ggtcaccgaa agggcggtcg gacaccgccg tccgctttcc cgctgtatag
      361 acgggcatat gctcaaggag caggcagcta tcgggaacct cgccgctgac ccgccgcccg
      421 tgcaccgtgc gctgcatgtc ccatccctgg acatagtcca cggcgttcga cccgaagcct
      481 gcgtgagcgt aaacgagttc agcatccaca acatcgagac tacgacgcga gtgagaccac
      541 cgcgtcggct gggaacaaac caaatcgtga tcaatgaggc agaactgcgg gagatgaccc
      601 ccagtgagcg acgcaagctg gcgcggtcgc tcaccaccgt cgactatccg caccccttgt
      661 tggggattta cctggcacga ggccgtcgac ttggcgccct cgtatcgatc atcgcctgca
      721 ccgtgctggt gggctggatc atcgtcctcg tcctcaccct gcatgactct ttccacgccc
      781 agcactggac gggcgcctgg gtcgggttcg acgtgatcct gctgttcgcg ttcgccgcga
      841 ccgggtgggc gttctggcgc ggccgccaga tcgttatcgc ctgcctgatt gccacgggaa
      901 ccctgctgtg ctgtgacgcc tggttcgacg tcgtcctcga cgcgggcacc tccgacgtgt
      961 ggctaagcgt cgcgtcggcg gtcgtcgtcg aactgccgct ggcgttcctc atgttcaacg
     1021 cggcccgccg cctcatccgg ctgtccgccg tcgtcgcggt gagcgaggcc ggcgactcga
     1081 gcgactgcga ccacctgccc gaggacgagc tgccgcccct gtggaagatc ccgctgttcg
     1141 gggtgggcag gcgcccggcg gcccgggcca gccaaacgcg ggccatcgaa acccgcccca
     1201 gcgaaacccg gcccatcgaa acccgggcca ccgaaacacg ggcaccgcgc acccgttcgg
     1261 atgaatatca cggcgccagc ggtagacccg caccgatcac ggacttagcc tcacagccat
     1321 ggctaccgac ttttccgagg aaacggcggc ggacgcggtc atcgagtcct tcgcgcccga
     1381 caccgacccg cggctccgcg agatccttgc ctcactggtc aagcacctgc acgcgttcgc
     1441 ccgcgacgtc gaactgacga ttcccgagtg ggagaaagcg gtcgacttcc tcaccaggac
     1501 cgggcacaag agcgacgacg agcggcagga gttcatcctg ctgtcggacg tcctcgggct
     1561 gtcgatgctg gtcgagacga tctcgaacag gaagttcggc gtcgcgaccg agtcgaccgt
     1621 gctcggcccg ttccacgtgg tccagtcgcc ggtccgcgcg ctcggcgacg acatcgacct
     1681 ggtgaaggac ccgcacgcca caccgtgcgt ggtcaccggg cgggtcgtct cgaccgacgg
     1741 cacgccgctc cccggcgcga gcatcgacgt gtggcaggcc aacgaccagg gcttctacga
     1801 tgtgcagcag cccgacgtgc agccgcgcac caacggccgc ggcctgttca ccgccgatga
     1861 gaacggcggg ttctggttcc gcacgatcgt gccgagctac tacccgatcc cgaccgacgg
     1921 cccggttggc gaggtgctgc tggcgacggg caggcacgcg ttccgtccgg cgcacatcca
     1981 cttcatcgtg tccgctccgg gataccgcga cctgaccacg cacatcttcg tggccggcag
     2041 tgagtacatc gagtccgaca cggtcttcgc ggtgaagaag tccctggtgg tcgatttcga
     2101 agaggtcgac gacccggccc tggcggagac atggcgggtt aaagtcccgt tccggcacgc
     2161 cgacttcgac atcgtcatgc agccgagcgc ctaactccga gggcgcaacg ggtctctcgc
     2221 ccgtcgtcag cgagggagtt cggccgctag cgccgcgccg atcgtcgggt agctgaactc
     2281 gaagccggcc tcgagcagac gcgcgggcag cacccgcgcg ctggtcagca gttccgagga
     2341 catttcgccg agggccagct tcagcagtgg cgcgggcacg cggagccagg ggaagtcggg
     2401 gcgccggaac gcggcgtgca gcgcggcggt gagctcgccg ttcgtcaccg cgttcggcgc
     2461 ggtgaggtta tacgggccgg tccgttcgtc cgcgccgggg ccgtcgagca ggaagcggat
     2521 ggccctgatc tcgtcggtca gcgagatcca gctcattacc tggtacccgt tgccgaagcg
     2581 cggcagcagg ccgaagcggg cgggcggcgc cagccgaccg agcacgccgc cgcccgcgcc
     2641 gagcacgagc ccgctgcgca cgtgcgcgac gcggatgccc gccgcgcgcg ccgggtcggc
     2701 ggcggcctcc cagtcgtgca cgacccgcga gaggaacgcc ttgcccgccg gggtgctc
//
LOCUS       DASRSC010000308         4933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5693351,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000308 DASRSC010000000
VERSION     DASRSC010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5693351"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VFE39_15190"
     CDS             complement(<1..287)
                     /locus_tag="VFE39_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET6259982.1"
                     /translation="MNILLWHVHGSWTTAFVQGPHRYLLPTLPERGPWGGGQPAAWNW
                     PAGAVEMAPADLADAEVDVVVVQRPEELALAEDWHGCTPGRDVSVIYLEHNA"
     gene            536..2170
                     /locus_tag="VFE39_15195"
     CDS             536..2170
                     /locus_tag="VFE39_15195"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HET6259983.1"
                     /translation="MGIRDDLQRIAADTFGWPELRPEQLDAMEHVAGGHDVLAVLPTG
                     SGKSAIYQVPALLRNGPAVVVSPLIALQRDQREGLTESGAPDAVAVNSAQRAGETRAA
                     WDAVDAGDAEYLFLAPEQLARDEVMARLVAAGPALFVVDEAHCVSEWGHDFRPDYLRL
                     GEVVERLGHPPVVALTATAAPPVRADIVSRLGLRDHREVLTSFDRPNLRLAGHEYADA
                     AQRRAEVVERVVELGGVGLLYCATRKDTVAYAGALAERGVAAAAYHAGLRRARRDEVH
                     EQFMNGAMDVVVATSAFGMGIDKPDVRFVLHAASPASLDAYYQEIGRGGRDGDPAVAE
                     LHHHAHDLNLQRFLTARRPKPDALRAALSALDPERPLPPKEIGAASRLSPARRTAALN
                     LLEQAGAALADADGRFIRTGLDAAAAVDRAVTIAEQRREVVRSRIEMIRGYAETTDCR
                     RRLLLGYFGEQVTEPCGRCDNCDAGRSTRPDPAGSAEADAAGLGSEERVQHPQFGEGM
                     VMSTEDDRVTVLFAEHGYKTLSLAAVQENALLEPRE"
     gene            2286..3242
                     /locus_tag="VFE39_15200"
     CDS             2286..3242
                     /locus_tag="VFE39_15200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET6259984.1"
                     /translation="MPTPTRALGQEAQTPAQIPARGWWQVIRRAVKESTADHVSMLAG
                     GVAFFAFLAVFPALIAAVTLYGLIADPAEVARQVESLAGALPQETRPLIAGQLNSVVS
                     SSDSALGVGLVVSLLAALWSASSGTGNLIQAVNLAYDEDETRGFLKLRALALLLTLGA
                     IVFVLVTLTLVAVVPAVLDALPLGTAGRVIAQVVRWLLLVAVVIGALAVVYRVAPDRD
                     APRFRWVSVGALVAAVLWLIGSGAFSLYVNNFGSYNKTYGALAGVVVLMLWLYLTSYI
                     VLLGAEINAESERQTRQDTTRGDPRPIGERSAVAADEVAEGP"
     gene            complement(3256..3753)
                     /locus_tag="VFE39_15205"
     CDS             complement(3256..3753)
                     /locus_tag="VFE39_15205"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HET6259985.1"
                     /translation="MTQRFEFAALGSEIPEPRAPVPLRSDLLRVSVCEPRPGVLVVAP
                     AGEVDLSTVGLLRAASYRAVEAGPDRVVVDLSGLTFCGSTGLVVLMDTQSHAVAHGVT
                     FRTAAAGRPVRRVLQITGLLGVLAHCDSLDEALGDSIDEALGVTVDEAPGAAPDEALG
                     EPRQQ"
     gene            complement(3878..>4933)
                     /locus_tag="VFE39_15210"
     CDS             complement(3878..>4933)
                     /locus_tag="VFE39_15210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259986.1"
                     /translation="NDEAEALVRGIEQRVERETGTQLPPVHGSLRVTQRTSTGFGELT
                     RTVLWTYPRRTLLGLALLVGQAFLYNAVYFTFALVLATFFGVPSASSGLYLIPLALGS
                     LVGPLVLGRLFDTVGRRTMISVSYLGSGVLLIGAGWLFSAGVLSAWTLAAAWAAVFFL
                     ASCGASAAYRTVSEIFPMEIRAMCIALFYAVGTGLGGIVGPVLFGALVETGRAGSVAL
                     GYYVGAVVMIIGGVAELALGVEAEQRPLEDAATPLSAQPIATGADEDRDPTRRPVPTR
                     TYNPRPVDDPERDRELGAILQALGDGPLGPTELAERVDAAAWEPGRFTATLRYGLATG
                     ALLDDGRDDQVRARYHD"
BASE COUNT          740 a         1789 c         1737 g          667 t
ORIGIN      
        1 gcgttgtgct ccagatagat caccgacaca tcccgcccag gcgtacagcc gtgccagtcc
       61 tccgcgagcg ccagctcctc gggccgctgg acgacgacca cgtcgacctc ggcatcggcg
      121 aggtcggcgg gagccatctc gacagcacct gcaggccagt tccacgccgc gggctgaccc
      181 ccgccccatg gcccgcgctc gggcagcgtc ggcaggagat accggtgcgg gccctggacg
      241 aacgccgtgg tccaggagcc gtgcacgtgc cacagcagga tgttcacagt ggaggtgtgg
      301 ccagcagcgc gacgatcaaa cgctgagaag caaactgcta ctccttgtgt acgactacta
      361 cttcccgtag tcacccggaa ccagctcgac ggctcgccgg cgcgcgctcg tcgtgccgca
      421 cggctgtcgg gtttcccgag gtcatgggcc cacgggtacc cggtgtaggg cgggctaccc
      481 gacggttcac ccgaaggacg agtggcgggc atcgaaccgg gtaacgcacg ctgctgtggg
      541 catccgtgac gacctgcagc gcatcgccgc cgacaccttc ggctggccgg agctgcgtcc
      601 agagcagctc gacgccatgg agcacgtggc cggtggccac gacgtgttgg ccgtgctgcc
      661 caccgggtcg ggcaagtcgg cgatctacca ggtccccgcg ctgctgcgga acggtcccgc
      721 ggtcgtcgtc tcgccgctga tcgcgctgca acgggaccag cgcgagggcc tcacggagag
      781 cggtgccccg gacgctgtgg ccgtcaactc agcgcagcgg gcaggcgaga cgagggcagc
      841 atgggatgcg gtggacgccg gcgacgcgga gtacctcttc ctcgccccgg aacagctcgc
      901 gcgcgacgag gtgatggcgc ggcttgttgc ggcgggaccc gcgcttttcg tggtggacga
      961 ggcgcactgc gtgtcggagt gggggcacga cttccggccc gactacctgc ggctcgggga
     1021 ggtcgtcgag cggctcgggc atccaccggt cgtcgccctg acggccacgg ccgcgccgcc
     1081 ggtacgggcc gacatcgtca gccggttggg tctgcgcgac caccgcgagg tgctcacgag
     1141 cttcgaccga ccgaacctgc ggctggcggg tcacgagtac gccgatgcgg cccagcggcg
     1201 cgcggaggtc gtcgagcgcg tcgtcgagct gggtggcgtc ggcctcctgt actgcgcgac
     1261 acgcaaggac accgtcgcgt acgccggggc gctggcagag cggggcgtgg ccgcagccgc
     1321 ctaccacgcc ggtctgcggc gcgcccgacg cgacgaggtg cacgagcagt tcatgaacgg
     1381 cgcaatggac gtcgtcgtgg cgacctcggc gttcgggatg ggcatcgaca agccggacgt
     1441 ccgcttcgtc ctgcacgccg catcgcctgc gtccctggac gcctactacc aggagatcgg
     1501 ccgcggtggt cgcgacgggg accctgcggt cgccgagctg caccaccacg cgcatgacct
     1561 caatctgcag cggttcctca ctgcccgccg cccgaaaccg gacgcgctgc gcgccgcact
     1621 ttccgcactg gaccccgaac gcccgctgcc gccgaaggag atcggcgccg cgagcaggct
     1681 gtctcccgcc cgccgcacgg cggcgctcaa cctgctcgag caggccggag cggcgctggc
     1741 cgacgcggac ggccggttca tccgtaccgg cctcgacgcc gctgcggccg tcgaccgcgc
     1801 cgtgacgatc gccgagcagc gccgggaggt ggtgcggtcg cgcatcgaga tgatacgtgg
     1861 gtacgccgag accacggact gccgcaggcg gctgctgctg ggctacttcg gggagcaggt
     1921 caccgaaccg tgcggacgat gcgacaactg cgacgcgggg agatcgacgc gaccggaccc
     1981 tgccggatcc gccgaggcgg acgccgcagg cctcggctcg gaggaacggg tgcagcaccc
     2041 gcagttcggc gagggcatgg tcatgtccac ggaagacgac cgcgtgacgg tgctgttcgc
     2101 cgagcacggc tacaagacgc tgtcgctggc ggccgtgcag gagaacgcgc tgctggagcc
     2161 gagggagtga cgctgcctcc cgtcacggag tcaggatcgc cgacaccgga cggttgggac
     2221 cgcccgcagc ggctatccgc acgggcgtgt ccaggtccca ggaggaaaga cccaggagga
     2281 actccgtgcc cactcccacg cgcgccctcg gccaggaggc gcagacgccc gcccagatcc
     2341 ccgcacgggg ttggtggcag gtcatccgcc gcgccgtcaa ggagagcacc gcggaccacg
     2401 tgtcgatgct ggctggcggc gtcgcgttct tcgcgttcct cgccgtcttc cccgccctga
     2461 tcgccgcggt gacgctctac ggcctcatcg ccgacccggc agaggtggcc cgtcaggtcg
     2521 agagcctcgc cggcgccctc ccgcaggaga cccggccgct gatcgccggc caactcaact
     2581 ccgtggtctc cagcagcgac agcgccctcg gggtagggct cgtcgtgtcg ctgctggcgg
     2641 cgctgtggtc ggcgtcgagc gggaccggca acctgatcca ggcggtcaac ctggcctacg
     2701 acgaggatga gacgcgaggc ttcctgaagc tgcgcgcgct cgcgttgcta ctcaccctcg
     2761 gcgcaatcgt gttcgtgctc gtgaccctca cgctcgtggc ggtggtgccg gccgtgctcg
     2821 acgcgctgcc gctgggcact gcaggccggg tcatcgccca ggtggtccgg tggctgttgc
     2881 tggtggcggt ggtgatcggc gcgctggcgg tggtctaccg cgtagccccc gaccgcgacg
     2941 cgccccgctt ccgctgggtc agcgtcggcg cacttgtcgc cgccgtgctc tggctcatcg
     3001 gcagtggggc gttcagcctc tacgtcaaca acttcggcag ctacaacaag acctacggag
     3061 cgctggccgg ggtcgtcgtg ctgatgttgt ggctctacct caccagctac atcgtgctcc
     3121 tgggcgcgga gatcaacgcg gagtccgagc ggcagacccg ccaggacacc acccggggtg
     3181 acccaaggcc cataggcgag cggagcgccg tggcggcgga cgaggtggcc gagggtccgt
     3241 aacgcggtgc ggcgcctatt gttggcgagg ttcgccgagc gcctcatccg gagcggcgcc
     3301 cggtgcctcg tcgacggtaa cgcccagtgc ctcgtcgatg ctgtcgccca gagcctcgtc
     3361 gaggctgtcg cagtgcgcga ggacgcccag caggccggtg atctgcagca cccgtcgcac
     3421 cggccgtccc gccgccgccg tgcggaacgt caccccgtgg gcgaccgcgt ggctctgcgt
     3481 gtccatcagg acgacgaggc cggtcgaccc gcagaaggtc aggccggaca ggtcgacgac
     3541 cacccggtcc ggtcccgcct ccacggcccg gtacgaggcg gcgcgcagca ggccgacggt
     3601 ggagaggtcg acctcaccgg ccggtgcgac gacgagcact ccgggccgcg gctcgcagac
     3661 cgacacccgg agcaggtccg accgcagcgg aacaggagct cgcggctctg ggatctcgga
     3721 gccgagggca gcaaactcga agcgctgagt catccggtct ccatccggtg gtgggaggag
     3781 aaggggggac caccggcccc gcgcgtccgt caacgatgcc cgcagccgtg gcaccgcgca
     3841 actgcgccac cccgtcggac gaggcatgat gccgcgctca gtcgtggtat cgggcgcgca
     3901 cctgatcgtc tctgccgtcg tcgaggagcg caccggtcgc gagcccgtac cgcagcgtcg
     3961 cagtgaaccg gcccggctcc catgcggccg cgtccacccg ctcggccagc tccgtcggcc
     4021 cgagcggccc gtcgcccaac gcctgaagga tcgctcccag ctcccggtcg cgctccgggt
     4081 cgtccaccgg ccgggggttg tacgtgcgag tggggacggg ccgccgggtc gggtcccggt
     4141 cctcgtcggc accggtcgcg atcggctgcg ccgacagcgg cgtcgcggcg tcctccagcg
     4201 ggcgttgctc ggcctcgacc cccagcgcga gctccgcgac accaccgatg atcatcacga
     4261 ccgcgccgac gtagtagccc agcgccacgc tccccgcccg gccggtctcc accagtgcgc
     4321 cgaacagcac cggcccgacg atgcctccca agcccgtgcc gacggcgtag aacagcgcga
     4381 tgcacatcgc cctgatctcc atcgggaaga tctccgacac cgtgcggtag gccgcgctgg
     4441 ccccgcagga cgcaaggaag aagaccgccg cccaggcggc cgcgagggtc cacgcggaca
     4501 gcacccccgc cgagaacagc cagccggcgc cgatgaggag cacacccgat cccagatagc
     4561 tgacgctgat catcgtgcgg cgcccgaccg tgtcgaacag ccgcccgagc acgagtggcc
     4621 ccaccaggct ccccagcgcc aacgggatca ggtagagccc gctcgacgca ctcggcacgc
     4681 cgaagaaggt ggccagcacg agggcgaagg tgaagtagac ggcgttgtag aggaacgcct
     4741 gccccacgag cagtgccagc cccaacagcg tccgccgcgg ataggtccac aacacggtcc
     4801 tcgtgagctc gccgaaaccg gtgctcgtgc gctgcgtcac ccgcaggctc ccgtgcaccg
     4861 gagggagttg cgtcccggtc tcccgctcga cccgctgctc gatcccccgc accagcgcct
     4921 cggcctcgtc gtt
//
LOCUS       DASRSC010000309         9665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4385072,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000309 DASRSC010000000
VERSION     DASRSC010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9665
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4385072"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1562)
                     /locus_tag="VFE39_15215"
     CDS             complement(<1..1562)
                     /locus_tag="VFE39_15215"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HET6259987.1"
                     /translation="MTAMTVSGIDEAPTENARLLSWVREVAELTTPERVVWCDGSADE
                     WDRMTTQLVEAGTFVKLNEKKKPNSFWAASDPDDVARVEERTFICSALEADAGPTNNW
                     MDPAEMKATMTELYRGCMRGRTMYVIPFCMGPLDADEPFLGVEITDSEYVVVSMHIMT
                     RMGSAVLPLFDGPAGDRFVPALHSVGAPLEPGQQDVPWPCNETKYITHFPETREIWSF
                     GSGYGGNALLGKKCYALRIASVMARDEGWMAEHMLILKLISPEEKAYYVAAAFPSACG
                     KTNLAMLQPTVPGWRAETVGDDIAWMRFGEDGRLYAVNPEAGFFGVAPGTNWKTNPNA
                     MRTIEKGNSLFTNVARTDDGDVWWEGLEGDPQHLTSWLGEDWTPDSDKAAAHPNSRYT
                     TPISQCPVVAPEWEDPQGVPISAILFGGRRKTTVPLITEARDWQHGTFMGATMSSEKT
                     AAAKGGLGQVRRDPMAMLPFLGYNVGAYFQHWVNIGKGADADKLPRIFYVNWFRRGDD
                     GQFLWPGFGENSR"
     gene            2008..2454
                     /locus_tag="VFE39_15220"
     CDS             2008..2454
                     /locus_tag="VFE39_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259988.1"
                     /translation="MSDTALYVTLLAVSVALTFLVGRLLVTAGEPFLQEVFEDRSVTR
                     SVNLLLSVLFHLITLGVLAIISVMDLVGNDNPFQTFVARLGVVLLVLGVAYGVSMLVL
                     IRVRERRRADQISEHVQERLADRGFGSGPQGTGQPGPTPPTVTPGQ"
     gene            complement(2496..4043)
                     /locus_tag="VFE39_15225"
     CDS             complement(2496..4043)
                     /locus_tag="VFE39_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6259989.1"
                     /translation="MTVIAPARRTQSPAAPRAGHREWIGLAVLSLPTLLVAIDNTVLG
                     FALPAISAALAPSATQLLWLVDIYPLVLAALLVTMGTLGDRIGHRRTLMIGVAGFGTV
                     SAAAALATNALHLVGARALLGFFGAMLMPSALALLRTLFTDRAQRRLAVAIWATGFAG
                     GAALGPILGGVLLQHFWWGAVFLVNVPAMVALLVLATVALPESRRPVPGRLDLPGVLL
                     SLLAMGPTVLAITLVADSGITTTAALALLVGVGSGVLFVRRARRRLAAGEEPLLDVTL
                     FASAVLRLSALANATTMFALTGLLFFSAQYLLLVLGLSPIDAGLVLLPGFLVTMLAGL
                     AAARLGRMFPLRALVPAGLGLAAVGFLLCTTLRGDTSVAVLASAFILVGAGIGLSETI
                     TNDAILAAAPPDRAGSAAAVSETAYEIGAVLGTAVLGSVLTAVYRGTVEVPTSIAPGD
                     AHAARETLGGAVGTAARLSGQEGQALLASARDAFVTGIDVTALVGGLTLATVALVVLL
                     GLRSRAR"
     gene            4188..4733
                     /locus_tag="VFE39_15230"
     CDS             4188..4733
                     /locus_tag="VFE39_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HET6259990.1"
                     /translation="MASARDRVLDAYETLLIEHGAGAVTLDAVAAAASVSKGGLLYHF
                     ASKDALATGLLDRLRERSAADADAIRAAPDGAVAYYVRTSAPGGGVPGGVTRTYLAVL
                     RLADGTTSGQAVRDALAVVDADAYAALREELDDPVLAWLAQLVGDGLYLRTLTGTPLP
                     PGLHVEDLLERMRRYPLASRD"
     gene            complement(4788..5063)
                     /locus_tag="VFE39_15235"
     CDS             complement(4788..5063)
                     /locus_tag="VFE39_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259991.1"
                     /translation="MYTLLVGDAVGPPTVSVHTTADDAWRALDGEVRRRCGMRPRPRR
                     RMDAEAATRLADAWRAGEPDSRFWNVAVHRLPIPLPVIARAAISGAR"
     gene            complement(5218..6648)
                     /locus_tag="VFE39_15240"
     CDS             complement(5218..6648)
                     /locus_tag="VFE39_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HET6259992.1"
                     /translation="MTPTRASQVTVSVLAPQEVVERTLAAGAHLHGCTVLVRETSEAV
                     LRWANSTMTTNGHTTSGQVTVIAFVAVEGGIAAGVVSSSAAVTGAGQLEELVRAAEAA
                     ARGAGPARDAAPLVEPAGGDHRPDDSRWDDPAIETSIGVFGSFAHGLSEVLAGPHRQY
                     GFASHEVMTIWLGTSTGVRRRWVQPTGSVELNAKTPDLTGSAWAGLSTTDFGDVDVAA
                     LAATATRRLEWGKRRVTLPAGRYETLLPPSAVSDLMIYLAWSMEGRPAQEGRSALAGP
                     DGPRVGERLTGLPLTLASDPAAAGLEYEPFLTATRSGEGISVFDNGAPTRRVEWISQG
                     AVGQLAYSRAEAAEFGTTFTPAGDNLLLTGGSDASIADMVARTERGLLLTCLWYIREV
                     DPATLLLTGLTRDGVYLVEKGEVVGEVDHNFRFNYSPLDVLRRTSEVGATERTLPREW
                     KDWFTRAAMPPVRVPEFNMSSVSLGR"
     gene            complement(6645..8156)
                     /locus_tag="VFE39_15245"
     CDS             complement(6645..8156)
                     /locus_tag="VFE39_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HET6259993.1"
                     /translation="MPADRIDPEFRALPLAALADAALSRARELGAQHADLRVERIVSQ
                     SIDLRDGTVTGVADTITVGLAVRVVVDGVWGFASHVELTPARAAATAERAVAVARTLA
                     PVAVERVERADEPVHADAVWVSDYTVDPFTVPTREKVDLLTEWSQRLLASDGVDHVQA
                     GVAQVRENKFYADTAGTTTTQQRVRIDPSVTATAVNRAAGGFETMSSLAPPAGRGWEY
                     LGGDGWDWHGELAQLPELLAEKAKAPSVHAGAYDLVIDPTNLWLTIHESVGHATEYDR
                     AIGYEAAYAGTSFATPDGLGTLRYGSEHMHVTGDRTVQHGLSTIGFDDDGVATGEWDL
                     VRDGVLVGYQLDRTFAPRLGLDRSNGCAFADSPHHVPIQRMANVSLRPDPTHDTSTEE
                     LIAGVDRGIYVVGDKSWSIDMQRYNFQFTGQRFFRIEGGKLAGQLRDVAYQATTTDFW
                     GSLDAVGGRSTWLLHGAMNCGKAQPGQVAAVSHGCPSARFRGINVLNTQEEGQ"
     gene            complement(8202..8507)
                     /locus_tag="VFE39_15250"
     CDS             complement(8202..8507)
                     /locus_tag="VFE39_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259994.1"
                     /translation="MIEERRPGRVPDEGGPVDIAHGFDVTAGFDTDGDGRSDTAVTDD
                     GVDLVLLTDLDGDGFADQVLRIGPDGVVREVAPDPSAGAGTGVVDGLRSGADAGYEP"
     gene            complement(8504..9286)
                     /locus_tag="VFE39_15255"
     CDS             complement(8504..9286)
                     /locus_tag="VFE39_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259995.1"
                     /translation="MPDRYIDAVRARRVELRARRGLLTGGHRAELRRCLALSRIAAAA
                     EASGALAGLAREAADHIETGDRAARAQLPDLLAAAVATVAVDVHAGWAVALRPALRSI
                     ATERNLPIDPSWPRLPAPQLPAVPAAPVPARRVRSLLAGAADGAALWRLALFPVAMLP
                     LLGLPALAGPALAPLAAGAGVATVAATARARRTAAERARLRCHTEQVLAAAALAIDAD
                     LGRRLVELERTVGATLDAAVLRRRAAVDAELALLAPDNGGVQ"
     gene            complement(9359..>9665)
                     /locus_tag="VFE39_15260"
     CDS             complement(9359..>9665)
                     /locus_tag="VFE39_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HET6259996.1"
                     /translation="GFTGGHAVVTVSWTIAALVLLARGISRAALRITGLVLVTAAVAK
                     LVLFDLVALDGLARVAAFLGAGLVLLVAGTRYARLVAEAEAEAGPEQAAQGAPGNVS"
BASE COUNT         1420 a         3522 c         3388 g         1335 t
ORIGIN      
        1 cgggagttct ccccgaagcc cggccagagg aactggccgt cgtcgccgcg gcggaaccag
       61 ttgacgtaga agatccgggg cagcttgtcg gcgtcggcgc ccttaccgat gttcacccag
      121 tgctggaagt aggcgccgac gttgtagccg aggaacggaa gcatggccat cgggtcgcgg
      181 cggacctggc cgagtccgcc cttggccgcg gcggtcttct cgctcgacat cgtggcgccc
      241 atgaacgtgc cgtgctgcca gtcgcgggcc tcggtgatga gcggaaccgt cgtcttgcgg
      301 cggccgccga acaagatcgc tgagatcggc accccctgcg ggtcctccca ctccggcgcc
      361 acgacggggc actgcgagat cggcgtggtg taacgggagt tcgggtgcgc ggcagccttg
      421 tccgagtcgg gtgtccagtc ctcaccgagc cacgacgtca ggtgttgcgg gtcaccctcg
      481 agaccctccc accagacgtc gccgtcgtcg gtgcgggcga cgttggtgaa gagcgagttc
      541 cccttctcga tcgtgcgcat cgcgttgggg ttggtcttcc agttggtgcc gggcgcgacc
      601 ccgaagaacc cggcctcggg gttgaccgca tagaggcggc cgtcctcgcc gaaacgcatc
      661 caggcgatgt cgtcaccgac ggtctcggcg cgccagccgg gcacggtcgg ctgcagcatc
      721 gcgaggttcg tctttccgca tgccgacggg aaagccgccg ccacgtagta cgccttctcc
      781 tcggggctga tcagcttgag gatcagcatg tgctcggcca tccagccctc gtcgcgggcc
      841 atgaccgagg cgatccgcag cgcgtagcac ttcttgccca gcagcgcgtt gccgccatac
      901 ccggagccga agctccagat ctcgcgggtc tccgggaaat gggtgatgta cttggtctcg
      961 ttgcacggcc acgggacgtc ctgctggccg ggctcgagcg gcgcgcccac ggagtgcagc
     1021 gcaggcacga acctgtcgcc ggccgggccg tcgaacagcg ggagcaccgc gctacccatg
     1081 cgcgtcatga tgtgcatcga cacgacgacg tactcggagt cggtgatctc cacaccgagg
     1141 aacggctcgt cggcgtcgag cggccccatg cagaacggga tcacgtacat cgttcgaccg
     1201 cgcatacaac cgcggtagag ctcggtcatg gtggccttca tctcggccgg gtccatccag
     1261 ttgttggtgg gaccggcgtc ggcctcgagg gccgagcaga tgaaggtgcg ctcctcgacg
     1321 cgtgccacgt cgtccggatc ggacgccgcc cagaaggagt tcggtttctt cttctcgttc
     1381 agcttgacga acgtgcccgc ctcgacgagc tgcgtagtca tgcggtccca ctcgtcggcg
     1441 gacccgtcgc accacacgac gcgctcgggt gtggtgagct cggcgacctc gcgcacccag
     1501 gacagcaggc gcgcgttctc ggtcggcgct tcgtcgatcc ctgacacggt catcgctgtc
     1561 atgtttcctc gctccttgcc ggcgccccgg ccctgggaat gctgcgcgca cccgtccgtg
     1621 ctcggccggg tgtgcaggtg aaagatggaa actgacggta ccgccggggt ccggattccc
     1681 ccaccgctgg cgctgtcgat tgactcacag gaccgatcat gtaatcgatt cagaagaccc
     1741 gtaaacgttt tccagcggga attccacgtg gttgcccccg tccccgacca caccgggtga
     1801 ccagcgtgtt gtccgtcggg acgggtccga tcagacggcc tgggatcgga tgatggagac
     1861 acctaccaca gcctgcacat cccggcggcc caaaccggtg cgagcggctc atcgacgccc
     1921 agcggatcac gctccggacg tctacgctgt ggtctcggtc ccgtacaccg cgtagtgccc
     1981 tgatcacgtt cgatgccgga ggacccgatg agcgacaccg ccctgtacgt caccctgctc
     2041 gcagtgtcgg tcgcgctgac gttcctcgtc gggcgtctcc tcgtcaccgc cggtgaaccg
     2101 ttcctgcagg aggtcttcga ggaccgctca gtcacccggt cggtcaacct gctgctctcg
     2161 gtgctgttcc acctgatcac actcggcgtg ctggcgatca tctcggtcat ggacctggtc
     2221 ggcaacgaca acccgttcca gacgttcgtc gcgaggctcg gcgtggtgct gctcgtcctc
     2281 ggcgtcgcgt acggcgtctc gatgctggtc ctcatccgcg tccgggagcg ccgccgggcc
     2341 gaccagatct ccgagcatgt ccaggagcgg ctggccgacc gcggtttcgg gtccgggcca
     2401 cagggcaccg ggcagccggg cccgacgccg cccactgtca cgcccggaca gtgacgcccg
     2461 gggccccgcc cgccggcttc gttcaacgcc ctcggtcagc gagcccggct ccgtagcccg
     2521 agcaggacga ccagcgcgac cgtggccagc gtgaggccac cgaccagcgc cgtgacgtcg
     2581 atccctgtca cgaacgcgtc gcgtgcggag gcgagcaatg cctgcccctc ctgcccggag
     2641 agccgtgctg ccgtcccgac ggcacctccg agtgtctccc tggcggcgtg ggcgtcgccg
     2701 ggtgcgatgc tcgtgggcac ctcgacggtc ccccggtaca cagccgtcag cacgctgccg
     2761 agcacggcgg tgccgagcac ggcgccgatc tcgtaggccg tctccgagac cgcagccgcg
     2821 gagccggcac ggtccggagg cgcagcggcg aggatcgcat cgttcgtgat cgtctcggac
     2881 aggccgatcc ccgctccgac aagtatgaac gcactggcca ggacagcgac cgaggtgtcg
     2941 ccgcgcaggg tggtacacag caggaacccg accgcggcga gcccgagacc cgccggaacc
     3001 agtgcccgca gcgggaacat gcgccccagc cgcgcggcgg ccaaccccgc cagcatcgtc
     3061 acgaggaacc ccggcaggag cacgagcccg gcgtcgatcg gcgagagccc gagcacgagc
     3121 agcaggtatt gggcggagaa gaagagcagc cccgtcaggg cgaacatcgt ggtcgcattg
     3181 gcgagggcgg acagccgcag caccgcagac gcgaacagcg tcacgtcgag caacggctcc
     3241 tcgcccgccg ccaggcggcg gcgcgcgcga cgcacgaaca ggaccccgga ccccaccccg
     3301 accagcagcg cgagcgccgc cgtcgtggtg ataccgctgt cggccacgag agtgatcgcc
     3361 agcaccgtcg gccccatcgc caagagcgac agcagcacac ccggtaggtc gagccgtccc
     3421 ggcaccggcc tcctcgactc gggcagcgcc acggtggcaa ggacgagcag cgccaccatc
     3481 gccggcacgt tgacgaggaa caccgcgccc caccagaagt gctggagcag cactccgccg
     3541 aggatcgggc ccagcgcggc gccgccggcg aagccggtgg cccagatcgc gacggcgagc
     3601 ctccgttgcg cccggtcggt gaacagcgtg cgcagcagcg cgagcgcgga cggcatcagc
     3661 atggcgccga agaacccgag cagggcccgg gcacccacca ggtgaagggc gttcgtcgcc
     3721 aatgctgccg cagcagagac ggtgccgaac ccggcgacgc cgatcatgag cgtccggcgg
     3781 tggccgatgc ggtcgccgag ggtccccatc gtgacgagca gggcggcgag caccagcggg
     3841 tagatgtcga ccagccacag cagctgcgtc gccgacgggg cgagcgcggc gctgatcgcg
     3901 ggcagggcga agccgagcac ggtgttgtcg atggcgacca gcagcgtcgg cagagacagg
     3961 acggccaggc cgatccactc ccggtggccg gcgcggggcg cagcgggtga ctgggtgcgg
     4021 cgggcagggg cgataacggt catgtgtcct cggggaacgc ccgtcaggga cgggtgatga
     4081 tcggggggtt gcgcgttaat ataccgtcta gacggtacag ttcccctcgt tgttcccggt
     4141 cagcgcggcg acgtggcgga gcacacccgg cctcctacac tcggtcgatg gcaagcgccc
     4201 gggaccgcgt gctggacgcg tacgagacgc tgttgatcga gcacggcgcc ggcgcggtca
     4261 cgctcgacgc cgtggccgcc gccgcctcgg tctccaaggg cgggctgctc taccacttcg
     4321 cgtcgaagga cgcgctcgcc accgggttgc tcgaccgcct ccgcgagcgc agcgccgccg
     4381 acgccgacgc catccgggct gcgcccgacg gcgccgtcgc ctactacgtg cgtacgtcag
     4441 cacccggcgg cggcgtgccg ggcggagtca cccgcaccta cctcgccgta ctgcggctcg
     4501 ccgacggcac gacgtcaggc caggccgtgc gtgacgcgct ggccgtcgtg gacgccgacg
     4561 cctacgccgc gctgcgcgag gagctcgacg acccggttct cgcgtggctg gcccagctcg
     4621 tcggcgacgg gctctacctg cgcaccctca ccggcacgcc gttgcccccc ggtttgcacg
     4681 tggaggacct gctggaacgg atgcgtcggt acccgctcgc gagcagggac tgagctccgg
     4741 tcgtcgaacc catcggaaac ggcgtcacac cgcatctcgc cgccgagtca gcgggcacca
     4801 ctgatcgccg cgcgcgcgat cacgggtagc gggatcggca accggtgtac ggccacattc
     4861 cagaaccggg agtcgggctc accagcccgc cacgcgtcgg caagtcgtgt ggcggcctcg
     4921 gcatccatcc tccgccgcgg gcgcggtcgc atcccgcagc gcctacggac ctcgccgtcc
     4981 aacgcccgcc aggcgtcgtc ggccgtggtg tgcacgctca ccgtcggcgg gcctacggca
     5041 tccccgacga gcagcgtgta cacgctgacc acctcgaacg acacttctcg gcccttctct
     5101 cgtccacgac ccggcgacga cgtcggcgca cacaacagtc aacggcatct gggcctccgg
     5161 ggttcgcagc ggcacaccct cgagcaccat ccggtcacag atccgggccg gactcggtca
     5221 gcggccgagt gacaccgagg acatgttgaa ctccggaacc cgcaccggcg gcatcgccgc
     5281 gcgggtgaac cagtccttcc actcccgggg cagcgtccgc tcggtggcac cgacctctga
     5341 cgtgcggcgc agcacgtcga gcggggagta gttgaagcgg aagttgtgat cgacctcgcc
     5401 caccacctca cccttctcca cgaggtacac accgtcgcgg gtgaggccgg tgagcagcag
     5461 ggtggccggg tcgacctccc ggatgtacca gaggcaggtg agcagcagac cgcgctcggt
     5521 gcgcgcgacc atatcggcga tggatgcgtc cgacccgccg gtgagcagca ggttgtcgcc
     5581 tgcgggggtg aacgtcgtgc cgaactcggc agcctcggct cgcgagtagg ccagctgacc
     5641 gaccgcgccc tgactgatcc actccaccct gcgggtggga gcgccgttgt cgaagacgct
     5701 gatcccctcg cccgatcggg tcgcggtgag gaacggctcg tactccagcc cggcagcggc
     5761 cgggtcagag gccagcgtga gtggcaggcc ggtgagccgt tcccccacgc gcgggccgtc
     5821 cggaccggcc agcgcgctac ggccctcctg cgcggggcgt ccctccatcg accaggccag
     5881 gtagatcatc aggtcggaga cggccgacgg cggcaggagg gtctcgtacc gtcccgccgg
     5941 aagcgtgacg cgtcttttcc cccactccag ccggcgggtc gcggtcgctg ccagggccgc
     6001 cacgtcgacg tcgccgaagt ccgtggtcga caacccggcc caggcggacc ccgtgaggtc
     6061 gggcgtcttc gcgttgagct ccaccgatcc cgtcggctgc acccagcggc gccgcacgcc
     6121 cgtggacgtg ccgagccaga tcgtcatcac ctcgtggctc gcgaacccgt actgccggtg
     6181 ggggccggcc aacacctcgg acagcccatg ggcgaacgac ccgaagaccc cgatcgacgt
     6241 ctcgatcgcc gggtcgtccc accgggagtc gtccgggcgg tgatcgccgc ctgccggttc
     6301 gaccagaggc gcggcgtcgc gggccgggcc cgcacctcgc gccgccgcct cggccgcccg
     6361 caccagctcc tcgagctgcc ccgccccggt gacggcggcg ctggagctga cgacaccggc
     6421 cgcgatgcct ccctcgacgg cgacgaacgc gatcaccgtg acctggccgg acgtcgtgtg
     6481 cccgttcgtg gtcatcgtcg agttggccca gcgcagcacg gcctcactgg tctcgcgcac
     6541 gagcacggtg cagccgtgga ggtgcgcccc ggcggccagc gtgcgctcca cgacctcctg
     6601 cggcgcgagc accgacaccg tcacctggct cgccctcgtc ggggtcactg accctcctcc
     6661 tgcgtgttga gcacgttgat gccgcgaaag cgcgctgacg ggcacccgtg gctgacggcc
     6721 gcgacctgcc ccggctgcgc cttcccgcag ttcatcgcgc cgtgcagcag ccatgtcgac
     6781 cggcccccca ccgcgtcgag cgaaccccag aagtcggtgg tggtggcttg gtaggcgaca
     6841 tcgcgcagct ggcctgcgag cttcccgccc tcgatgcgga agaagcgctg gccggtgaac
     6901 tggaagttgt agcgctgcat gtcgatcgac caggacttgt cgccgacgac gtagatcccc
     6961 ctgtcgacgc cggcgatcag ctcctccgtg ctggtgtcgt gcgtgggatc gggacgcagc
     7021 gacacgttgg ccatccgctg gatcggcacg tggtgcgggg agtcggcgaa ggcgcagccg
     7081 ttggagcggt cgagtcccag ccgcggcgcg aacgtccggt cgagctggta accgaccagc
     7141 acgccgtcgc gcacgagatc ccactcgccc gtggcgacgc cgtcgtcgtc gaagccgatg
     7201 gtcgagaggc cgtgctggac cgtccggtcg cccgtgacgt gcatgtgctc cgagccgtag
     7261 cgcaacgtcc cgagcccgtc cggcgtggcg aaactggtgc cggcgtaggc ggcctcgtag
     7321 ccgatggccc ggtcgtactc cgtggcatga ccgacggact cgtggatagt gagccacagg
     7381 ttcgtcgggt cgatgacgag gtcgtaggca ccggcgtgga ccgacggggc cttggccttc
     7441 tcggcgagta gctcgggcag ctgggcgagc tcgccgtgcc agtcccagcc gtcgccgccc
     7501 aggtactccc agccccggcc ggccgggggc gcgagggaac tcatcgtctc gaacccacct
     7561 gcggcgcggt tcacggcggt cgccgtgacg gacgggtcga tccggacgcg ctgctgcgtg
     7621 gtcgtggtgc ccgccgtatc ggcgtagaac ttgttctccc gcacctgcgc gaccccggcc
     7681 tgcacgtggt cgacgccgtc cgaggcgagc agccgctgcg accactcggt gagcaggtcc
     7741 accttctccc gggtgggaac cgtgaagggg tcgacggtgt agtccgacac ccagacggca
     7801 tccgcgtgta ccggctcgtc ggcccgctcg acgcgctcga ccgccaccgg cgcgagcgtg
     7861 cgggccacgg ccacagcacg ctccgccgtg gccgccgccc tggccggggt cagctcgacg
     7921 tgcgacgcga aaccccacac gccgtcgacg acgacgcgca ccgcgagccc cacggtgatg
     7981 gtgtccgcga cgccggtgac ggtgccgtcg cggaggtcga tggactgcga cacgatccgc
     8041 tcgactcgca ggtcggcgtg ctgcgcgccc agctcgcgcg cccggctcaa ggcggcgtcg
     8101 gccagagcgg cgagcggcag ggcccggaac tccgggtcga tgcggtctgc gggcatacct
     8161 cgcaacctac tggccggccg gatgagcacg tcgcgccgac ctcagggctc gtacccggcg
     8221 tcggccccgc ttcgcaggcc gtcgacgacg ccggtgcctg cgcccgccga cgggtccggc
     8281 gccacctccc gaacgacgcc gtccgggccg atccgcagga cctggtcggc gaacccgtcg
     8341 ccgtcgagat cggtgagcag gacgagatcg acgccgtcgt cggtgaccgc cgtgtcggag
     8401 cggccgtccc cgtcggtgtc gaagcctgcc gtgacgtcga acccgtgagc gatgtcgact
     8461 ggcccaccct cgtcgggcac gcgacccggg cgtcgctcct cgatcattgg acgccgccgt
     8521 tgtcaggggc cagcagggcc agctcggcgt cgaccgcggc gcggcgccgg agaaccgcgg
     8581 catcgagggt tgcacccacc gtgcgctcga gctcgaccag tcgccgtccg agatcggcgt
     8641 cgatcgcgag tgcggccgcg gcgagcacct gctcggtgtg gcagcgcagc cgtgcgcgtt
     8701 cggcggctgt gcgccgcgcc cgagcggtgg cggccaccgt ggcaacgccg gcaccggcgg
     8761 caagcggggc cagcgccggc ccggcgaggg ccggcagccc gagcagcggc agcatcgcca
     8821 ccgggaacag cgcgagccgc cagagcgcgg ccccgtcggc agcaccggcg agcagcgacc
     8881 gcacgcgacg ggcggggacc ggcgctgccg gcaccgccgg gagctgcggt gccggcagcc
     8941 ggggccagct cggatcgatc ggcaggttcc gttcggtcgc gatgctgcgc agcgcggggc
     9001 gcagggccac cgcccatccg gcgtggacgt cgaccgcgac ggtggcgaca gccgcagcga
     9061 gcaggtcggg cagctgcgcc cgggccgcgc ggtcgccggt ctcgatgtga tcggccgcct
     9121 cccgggcgag cccggccagc gccccgctcg cctcggctgc cgcggcgatc cggctcaggg
     9181 ccaggcacct gcgcagctcc gcccggtgac cgccggtcag caggccccgc cgcgcgcgca
     9241 gctcgacgcg gcgagcccgg acggcgtcga tgtaccgatc aggcattccc agagcgtcgc
     9301 agcgccggac cgtcggcggg gccgaacggt cgtagcggtg tgcgatcgcg aacgcggctc
     9361 agctcacgtt tcccggggcg ccctgcgccg cctgttccgg gcccgcctcc gcctcagcct
     9421 cggcgacgag ccgtgcgtag cgggtgcccg ccaccagtag caccaacccg gcaccgagga
     9481 acgccgccac cctggccagt ccgtcgagcg cgacgaggtc gaacagcacc agcttcgcga
     9541 cggcggcggt gaccagcacg agcccggtga tccgcagcgc ggccctgctg atcccgcggg
     9601 ccagcaggac gagcgcggcg atcgtccagg acaccgtcac cacggcgtgc ccgccggtga
     9661 agccg
//
LOCUS       DASRSC010000310         9977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4060323,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000310 DASRSC010000000
VERSION     DASRSC010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9977
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4060323"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(416..2434)
                     /locus_tag="VFE39_15265"
     CDS             complement(416..2434)
                     /locus_tag="VFE39_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HET6259997.1"
                     /translation="MPNRLAAATSPYLLQHADNPVDWWEWSDEAFVEAAHRDVPVLLS
                     VGYAACHWCHVMAHESFEDEDTAKQVNAEFVAVKVDREERPDIDAVYMAATQALTGQG
                     GWPMTCFLTPSGEPFHCGTYYPPTPRSGMPGFRQLLGAVTKAWRDDGERVRRSAGEIA
                     ARLADNAAATLPPGAVDPDSLDRAAATLGELFDPRAGGFGGAPKFPPSMALEFLLRHH
                     ERTGSGDALRLAALTCERMARGGMYDQLAGGFARYSVDAAWVVPHFEKMLYDNGLLLR
                     VYAHLARITGAALPRRIAEETAAFLLRDLRTAEGGFASALDADTEGVEGLTYAWTPAQ
                     LREVLGEDDGSWVASLLTVTDRGTFEHGSSTLQLLADPDDPERWERVRPTLLASRNTR
                     AQPDRDDKVITAWNGMAILALAEGGAALGHPEWVDAAAAAADLLLRLHVVDGRLRRSS
                     RDGVVGNAAGVLEDHGCLADALLALHQATGSPRWLTAAGEVLDIALEHFADPERPGTF
                     YDTADDAEALLHRPREFTDNATPSGSAAMASALLTASVLVDGPARYRDAAEAALRSAG
                     TLAEKHPRFAGHWLTAAEAIVRGPLQVAVVGQDSDPARDALLAHARHIAPGGTVIVPG
                     QPDAPGVPLLADRPLVHDVPAAYVCRGFVCDRPVTTVEELTASLGLRP"
     gene            complement(2448..2789)
                     /locus_tag="VFE39_15270"
     CDS             complement(2448..2789)
                     /locus_tag="VFE39_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6259998.1"
                     /translation="MSPFLAEPLLIDPLLIDHGVTAAGPIVLAMPVQDPAPPPGKGEE
                     FGEASPVALVVIVLLAIITALLIRSMTKRIRRLPPSFDPTDKKPGGSADAEQGRGDGE
                     LGSGDAGSDRA"
     gene            complement(2811..3728)
                     /gene="mca"
                     /locus_tag="VFE39_15275"
     CDS             complement(2811..3728)
                     /gene="mca"
                     /locus_tag="VFE39_15275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010126 - mycothiol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol conjugate amidase Mca"
                     /protein_id="HET6259999.1"
                     /translation="MANFAPHSAEPLRLMTVHAHPDDESSKGAATSARYVAEGHEVMV
                     VTCTGGEAGSILNPAMERPEVLADLPAIRRAEMARAAEILGVQHRWLGFVDSGLPEGD
                     PKPPLPEGCFAVQDLDVVTRPLVAAMREFRPHVVVTYDENGGYPHPDHIRCHEISVAA
                     FDAAGDADRYPGTGAPWQPLKLYYSHGFSKARLEAFHESLLARGLESPYAEWLTNWPA
                     DRRDPGERVTTRVQCADWFDVRDKALIAHATQIDPTSRWFLVPLDIQREVWPFEEYEL
                     VRSFVDSRVPEDDLFAGIDAARVAVRPAV"
     gene            4269..4754
                     /gene="greA"
                     /locus_tag="VFE39_15280"
     CDS             4269..4754
                     /gene="greA"
                     /locus_tag="VFE39_15280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET6260000.1"
                     /translation="MTDTQVTWLTQEAYDRLKQELDELIANRPVIAAEINARREEGDL
                     KENGGYHAAREEQGQQEARIRQLQELLRTAHVGTPPTSSDVAAPGMVLKIRYDDEDSE
                     TVLLGSREEGSHGDLQVISPNSPLGAALLGARVGETREYQLPDGGSMKVSLISAEPFR
                     G"
     gene            complement(4784..6028)
                     /gene="ilvA"
                     /locus_tag="VFE39_15285"
     CDS             complement(4784..6028)
                     /gene="ilvA"
                     /locus_tag="VFE39_15285"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006549 - isoleucine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HET6260001.1"
                     /translation="MDTAGPVAALPVGLADVRSARDLLQGVVSPTPVAGSRALSELCG
                     GPVWLKCENLQRTGSFKIRGAYTRMARLDEEQRARGVVAASAGNHAQGVALAAQMLGI
                     EATVFMPERAAIPKVGATRGYGAQVHLVGETIEGSIAAATEFAERTGAVLVHPFDHPD
                     VIAGQGTVGLELLEQVPDVRTVLVCTGGGGLIGGIAAAVKGQRPDVAVVGVQAEGAAA
                     WPASLAAGGPVALESMRTIADGIAVGRPGDVTFPQVAALVDEILTVDEDALSRALLHC
                     LERAKLLVEPAGAAAVAALLQHPRRFATPAVAVLSGGNVDPLVLLHVIQHGMASAARY
                     LSLRVRVGDRPGALAELLALVGSLGANVLDVEHSRIAGTLALGDVEVALALETRGPEH
                     CAELVDALRVAGHAVSEVAGAR"
     gene            6126..6353
                     /locus_tag="VFE39_15290"
     CDS             6126..6353
                     /locus_tag="VFE39_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260002.1"
                     /translation="MTRVRAMAPLLVAAVLAAVAVATVEGAGCDDPGRYELGVNGYEL
                     VGGCIAPGDLMVPEPVQQPPAPTDPGTPAKS"
     gene            complement(6376..7524)
                     /locus_tag="VFE39_15295"
     CDS             complement(6376..7524)
                     /locus_tag="VFE39_15295"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET6260003.1"
                     /translation="MRGFSTNAIHAGQEPDPTTGAVITPIHQSSTFAQDGVGGLRGGY
                     EYSRSANPTRTALQECLAALEGGRHALAFGSGMGASDVLLRVLLRPGDHIVIPHDAYG
                     GTFRLVDKVLQPWGVEYTPVDLADVDALRAALRPTTRVVWCETPTNPLLGIADIAALA
                     DVVRTASARLVVDNTFASPYLQTPLGLGADVVLHSTTKYVGGHSDVVGGALVTGDDEL
                     AERLRFTQNSVGSVPGPFDAWLTLRGVKTLAVRMERHSDNAERVAELLSGHRGVEAVL
                     YPGLPEHPGHEVAAKQMRRFGGMVSFTVAGGRDAALRVCAGTKIFTLAESLGGVESLI
                     EHPGAMTHASVAGSVLEVPDNLVRLSVGIEDVEDLVEDLRQALDAATR"
     gene            complement(7614..8990)
                     /locus_tag="VFE39_15300"
     CDS             complement(7614..8990)
                     /locus_tag="VFE39_15300"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HET6260004.1"
                     /translation="MRYVEHVADLVGNTPLVRLGAVAEGIVAPVLAKVEYFNPGGSVK
                     DRIALRMVEAAEASGELRRGGTIVEPTSGNTGVGLAMIAQRKGYSCVFVCPDKVSEDK
                     RNVLRAYGAQVVVCPTAVEPDHPDSYYRVSDRLVREIEGAWKPNQYANVNNPESHYLG
                     TGPEIWEQTDGRITHFVAGIGTGGTISGTGRYLKEVSDGRVQVIGADPEGSVYSGGGG
                     RPYLVEGVGEDFWPTTYDKNICDRIVAVSDADSFHMTRRLAREEALLVGGSCGMAAEA
                     ALRVARELSADEAERAVVVVLLPDGGRGYLGKVFDDSWMATYGFLPPAQGATVGDVLR
                     RKDGSIPDLVHVHPNETVADAVSYLREFHVSQMPVVRAEPPVMAAEVAGAVVERDLLD
                     ALFTGRATLSDRLEKHMSAPLPTIGAGEALETAMTAFATADAALVLVDGKPAGVVTRS
                     DVLAFLGS"
     gene            9287..9916
                     /gene="shbA"
                     /locus_tag="VFE39_15305"
     CDS             9287..9916
                     /gene="shbA"
                     /locus_tag="VFE39_15305"
                     /inference="COORDINATES: protein motif:HMM:NF007230.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor ShbA"
                     /protein_id="HET6260005.1"
                     /translation="MPSVGDDMSEVEDSDRAEAVRLARRDAELDSLASRAASGHRPSL
                     DQLLRYVRPLVVRYCRARIGSGAIGLATPEDVAQDVLLGLYNALGRWRPDETRVMAFV
                     YGIASNKVVDAYRAAGRNRSVPTDMLPDAADTGAGPEAIAVLGSQIGELRRLLEQLPE
                     HHREVLVLRIALGLSAEETARTIDSTAGAVRVTQHRALTKLRQLAANRG"
BASE COUNT         1454 a         3609 c         3584 g         1330 t
ORIGIN      
        1 acccgtcggg caaccgcccc tgcagccacg ccgcggcgtc ctcggcaccg ggcaggtctg
       61 cctgctgcca cccaccgcgc ccggggccac ggccccctcg gcttccgggt ccgtgccacc
      121 cgcggtgcat ccgtccggtc atcttcgcca cctccgcttg cctcgtcgat tacgagatta
      181 cacagcaagc gtgtccgcgt gcaggatttc gcgcggggtg aacctcacag ccggcccgcc
      241 gcgcccaacg ggcgactcgc ggaccagaac cgggcactcg cgggcgcgat cccggcgact
      301 cgcgggcgca atccccgact cgcgggcgca atcccggcga ctcgcgggcg caatccgggc
      361 gactcgcggg cgggttttgg gcgactctcg ggcggggttt gggcgactcg cggggttagg
      421 ggcgtaggcc caggctcgcg gtcagctcct ccaccgtcgt caccgggcgg tcgcagacga
      481 agccgcggca gacgtacgcc gcgggcacgt cgtgcacgag cgggcggtcg gccagcagcg
      541 ggaccccggg cgcgtccggc tggccgggga cgatgacggt gccgccgggc gcgatgtgcc
      601 gggcgtgtgc gagcagggcg tcccgagccg ggtcgctgtc ctggccgacg accgccacct
      661 gcagcggccc ccgcactatc gcctcggccg cggtgagcca gtgcccggcg aaccgcgggt
      721 gcttctccgc cagcgtgccc gcgctgcgca gggccgcctc ggcggcgtcc cggtaccgag
      781 cgggaccgtc caccagcacc gaggcggtca gcagggcgga cgccatcgcg gcgctaccgc
      841 tgggggtggc gttgtcggtg aactcgcgtg gccggtgcag cagcgcctcg gcgtcgtcgg
      901 ccgtgtcgta gaaggtgccg gggcgctcgg ggtcggcgaa gtgctcgagc gcaatgtcga
      961 gtacctcacc ggcggccgtg agccagcgcg gcgagccggt ggcctggtgc agcgcgagca
     1021 gcgcgtcggc cagacacccg tggtcctcca gcaccccggc agcgttgccc accacaccgt
     1081 cccgcgacga gcgacgcagc cgcccgtcca cgacgtgcag ccgcagcagg agatcggcgg
     1141 ccgcggccgc ggcgtcgacc cactccggat gaccgagtgc cgccccaccc tccgcgagcg
     1201 cgaggatggc catgccgttc caggcggtga tcaccttgtc gtcccggtcc ggctgggccc
     1261 gggtgttgcg cgacgcgagc aatgtggggc ggacccgttc ccaccgctcc gggtcgtccg
     1321 ggtcggcgag cagctgcagc gtggaagatc cgtgctcgaa ggtgcccctg tccgtcacgg
     1381 tcagcaggga cgcgacccat gacccgtcat cctcgcccag cacctcgcgc agctgggccg
     1441 gcgtccaggc gtaggtcagg ccctcgacgc cctcggtgtc ggcgtcgaga gccgaggcga
     1501 agccgccctc cgccgtacgc aggtcgcgca gcaggaaggc cgcggtctcc tcggcgatcc
     1561 gcctgggaag cgcagcgccc gtgatccgcg cgaggtgggc gtagacccgc agcagcagac
     1621 cgttgtcata cagcatcttc tcgaagtgcg gcacgaccca cgcggcgtcg acgctgtagc
     1681 gggcgaagcc accggccagc tggtcgtaca tcccgccgcg cgccatccgc tcacaggtga
     1741 gcgccgccag gcgcagcgcg tcgcctgaac cggtgcgctc gtggtggcgc agcaggaact
     1801 ccagcgccat cgacggcggg aatttcggcg ccccaccgaa cccgccggcc cgcgggtcga
     1861 acagctcgcc gagggtggcg gcggcacggt ccagcgagtc cgggtcgacg gctccgggag
     1921 gcaacgtcgc ggccgcattg tcggccagcc gggcggcgat ctcccccgcg gaacggcgga
     1981 cgcgctcgcc gtcgtcgcgc cacgccttcg tgacggcgcc gaggagttgg cggaacccgg
     2041 gcatgccgga gcggggggtg ggcgggtagt aggtaccgca gtggaacggt tccccggacg
     2101 gggtgaggaa gcaggtcatg ggccagccac cctggccggt cagggcctgg gtggccgcca
     2161 tgtagacggc gtcgatatcc gggcgttcct cgcgatcgac cttgacggcg acgaactcgg
     2221 cgttgacctg cttcgcggtg tcctcgtcct cgaacgactc gtgggccatc acgtggcacc
     2281 agtggcaggc cgcgtagccc accgacagca acaccggcac atcacggtgg gccgcctcga
     2341 cgaacgcctc gtccgaccac tcccaccagt cgacagggtt gtccgcgtgt tggagcaggt
     2401 acgggctggt tgcagcggca agccggttcg gcatgccgcc caccctgtca ggcccggtcg
     2461 ctcccggcat ccccggagcc gagctcgcca tccccacgcc cctgctcggc gtcggcgctg
     2521 ccgccgggct tcttgtcggt ggggtcgaag ctcggcggta gacgccggat gcgcttggtc
     2581 atcgacctga tcaggagcgc cgtgatgatc gccagcagca cgatgacgac gagcgcgacg
     2641 ggcgaggcct cgccgaactc ctcgcccttc ccgggcggcg gcgccggatc ctgcaccggc
     2701 atcgccagga cgatgggccc ggccgccgtg acgccatgat cgatcagcag ggggtcgatc
     2761 aggagcggct cggccaggaa cggactcacg cggcgaacgg tacgcgctgg tcagaccgcg
     2821 gggcgcaccg ccaccctggc ggcgtcgatg ccggcgaaca ggtcgtcctc cggaacgcgc
     2881 gagtcgacga agctgcgcac gagctcgtac tcctcgaacg gccacacctc gcgctggatg
     2941 tcgagcggga cgagaaacca gcggctggtc gggtcgatct gagtggcgtg ggcgatcagc
     3001 gccttgtccc gcacgtcgaa ccagtcggca cattgcaccc gggtggtgac gcgctcgccg
     3061 gggtcgcgcc ggtcggcagg ccagttcgtc agccattcgg cgtagggcga ctcgagccca
     3121 cgggcgagga gggactcgtg gaaggcctcg agccgggcct tggagaaccc gtgggagtag
     3181 tacagcttga gcggctgcca gggagcaccg gtgccggggt accggtcggc gtcgccggcg
     3241 gcgtcgaacg ccgccacgga gatctcgtgg cagcggatgt gatccgggtg cgggtagccg
     3301 ccgttctcgt cgtaggtgac cacgacgtgc gggcggaact cgcgcatcgc ggccacgagc
     3361 ggcctcgtga cgacgtcgag atcctgcacc gcgaagcagc cctcgggcag cggtggcttg
     3421 ggatcaccct cggggagccc ggagtcgacg aagccgagcc agcggtgctg cacgccgagg
     3481 atctcggcgg cccgggccat ctcggcacgt cggatcgcgg gcaggtcggc gagcacctcg
     3541 ggccgctcca tcgcggggtt gaggatgctc ccggcctcac cgccggtgca cgtgacgacc
     3601 atgacctcgt ggccctcggc gacgtagcgc gccgacgtgg cggcaccctt gctggactcg
     3661 tcgtcggggt gcgcgtgcac ggtcatgagc cgcagcggtt cggcactgtg cggagcaaaa
     3721 ttggccatcg gtcggcgcgg tccttccgga tgagcaggtt cagtgcggca ggtcgtgccg
     3781 ggaacgatcg ttgcagagca tcccgacaac ctgcccgtcg actgtggaac gtctcagacg
     3841 tgcccgtgct tccccctccg gttcgtgacg ctccgctacc ccccgagtac gctggaagcg
     3901 ctggggcccc tcgatgcttc cccaatcccg cggcgcgcga gaggcggacg tgcgtggcta
     3961 ctgtgagcgg tcgccaggcc cctcgaacgg cacaaccggg ccggcccgcg tcgtcggcgc
     4021 actgccactg acctgcagtg atgatgttcc cgggaacttt ctcttcaccc caaccgttcg
     4081 tcgaatggct gcgacgagcg gctggcaccg tgttaccgtg aaccgcacac ggcccgcagg
     4141 cgggtcgtgt ttttccttac tcggggctat gccgcccacg agaccggggc cgcgaaggcg
     4201 gcggttcccg tccgcgggcg aacggtccgt ggctgcatgg acctggacag gctggaggag
     4261 tgatgaccgt gacggacacc caggtgacct ggctgaccca ggaagcctac gaccggctca
     4321 agcaggaact cgacgagctg atcgccaacc gccccgtcat cgctgcagag atcaacgccc
     4381 gccgcgagga gggcgacctc aaggagaacg gcggctacca cgccgcgcgc gaggagcagg
     4441 gccagcagga ggcgcgcatc cgccaactcc aggagctcct gcgcaccgcg cacgtgggca
     4501 cgccacccac gtcgtccgac gtcgccgccc ccggcatggt gctgaagatc cggtacgacg
     4561 acgaggactc cgagacggtg ctgctgggct cccgcgagga gggtagccac ggcgacctgc
     4621 aggtgatctc gccgaactcg cccctcggag cggcgctgct cggcgcccgc gtcggggaga
     4681 cgcgggagta ccagctgccc gacggcggca gcatgaaggt ctcgctgatc tcggcggagc
     4741 cgttccgggg ctgaccgggc ccgtaacgcc accggagagc cgttcagcgc gcgccggcca
     4801 cctcggacac cgcgtggccg gcgacgcgca gcgcgtcgac gagctcggcg cagtgctcgg
     4861 gcccgcgggt ctcgagcgcg agcgccacct cgacgtctcc gagcgcgagc gtcccggcga
     4921 tccgggagtg ctcgacgtcg agcacgttgg cgccgagcga tccgacgagt gcgagcagct
     4981 cggccagagc gccgggccgg tcacccaccc gcacccgaag cgacaggtaa cgggccgccg
     5041 aggccatccc gtgctggatc acgtgcagca gcacgagggg atccacgttg ccccctgaga
     5101 gcaccgccac ggccggggtg gcgaaccggc gtggatgctg cagaagggcg gccacagccg
     5161 ccgcacccgc cggctccacc agcagcttcg cgcgctccag gcagtgcagc agggctcggg
     5221 acaacgcgtc ctcgtccacc gtcaggatct cgtcgaccaa cgccgccacc tgcgggaacg
     5281 tgacgtcccc cggccgtccg acggcgatgc cgtccgcgat ggtgcgcatc gactcgaggg
     5341 ccacgggtcc gccggcggcg agcgacgcag gccaggcagc ggcgccttcg gcctgcaccc
     5401 cgaccaccgc tacgtccggg cgctgcccct tcaccgcggc ggcgatgccg ccgatcagcc
     5461 caccgcctcc cgtgcagacg aggacggtgc gcacatccgg cacctgctcg agcagctcca
     5521 gcccgaccgt gccctgccct gcgatgacat ccggatggtc gaacggatgc accagcaccg
     5581 ctccggtccg ctcggcgaac tcggtggccg cggcgatgct gccctcgatc gtctcgccga
     5641 cgagatgcac ctgcgcgccg tagccgcgcg tggcgccgac cttcgggatc gccgcccgct
     5701 ccggcatgaa gacggtggcc tcgataccga gcatctgggc ggccagcgcc acgccctgcg
     5761 cgtggttgcc cgcgctggcg gccaccaccc cgcgggcgcg ctgctcctcg tcgaggcggg
     5821 ccatacgggt gtaggcaccg cgaatcttga aggaccccgt gcgctgcagg ttctcgcact
     5881 tcagccacac cgggccgccg cacagctcgg agagcgcacg ggacccggcc acaggggtgg
     5941 gcgagacgac cccttgcagc aggtcgcgag cgctgcggac gtccgcgagc cccacgggca
     6001 gcgcggcgac cggtccggcg gtgtccatga cggcatcctc gcaccgtcgg agcagcggcc
     6061 ggcggacgcc ggtagcctgg tgccaggggt ggcgccccga cggacgccgc tggcggggag
     6121 gtcggatgac tcgggtgcgg gcaatggcgc cgctgctcgt ggccgcggtg cttgctgcgg
     6181 tggcggtggc caccgtcgag ggtgccggct gcgacgaccc gggccgctac gagctgggag
     6241 tcaacggcta cgagctggta ggaggctgta tcgccccggg tgacctcatg gtgcccgagc
     6301 ccgtacagca gccgcctgca ccgaccgacc cgggcacccc cgccaagagc tgaggccgcc
     6361 ggacgctccc tcgggtcagc gggttgccgc gtccagcgcc tgccggaggt cctcgaccag
     6421 gtcctcgacg tcttcgatgc ccaccgagag gcgcacgagg ttgtccggta cctccagcac
     6481 cgaaccggcc accgaggcgt gcgtcatggc gccggggtgc tcgatcagcg actcgacgcc
     6541 accgagcgac tcggcgagcg tgaagatctt ggtgccggcg cagacccgca gggccgcgtc
     6601 gcggccaccc gccacggtga acgacaccat cccaccgaac cggcgcatct gcttcgccgc
     6661 cacctcgtgg ccggggtgct cgggcaggcc cgggtagagc acggcctcga cgccgcggtg
     6721 gccgctgagc agctctgcaa cgcgctcggc gttgtcgctg tggcgctcca tccgcaccgc
     6781 gagcgtcttg accccccgca aggtgagcca ggcgtcgaac gggccgggga cggatccgac
     6841 ggagttctgg gtgaaccgga gccgctcggc cagctcgtcg tcgccggtca ccagcgcccc
     6901 gcccaccacg tcggagtgac cgccgacgta cttggtggtg gagtgcagca ccacgtcggc
     6961 accgaggccg agcggggtct gcaggtaggg cgatgcgaac gtgttgtcga cgacgagccg
     7021 cgcggacgcg gttcgcacga cgtcggccag cgcggcgatg tcggcgatcc cgagcagcgg
     7081 gttggtgggg gtctcgcacc agacgacgcg ggtggtgggc cgcagcgcgg cgcgcagcgc
     7141 gtcgacgtcg gcgaggtcga ccggggtgta ctcgaccccc cacggctgca gcaccttgtc
     7201 gaccaaccgg aacgtgccgc cgtacgcgtc gtgcgggatg acgatgtggt caccggggcg
     7261 cagcagcacc cgcagcagga cgtcggacgc tcccattccg gatccgaacg cgagcgcgtg
     7321 ccgcccgccc tcgagtgcgg cgaggcactc ctgcagggcg gtacgggtgg ggttggcgct
     7381 gcgcgagtac tcgtagccgc cgcggagccc gcccaccccg tcctgtgcaa aggtggacga
     7441 ctggtggatc ggggtgatca ccgcaccggt ggtggggtcc ggctcctggc cggcgtggat
     7501 cgcattggtg gagaagccgc gcataacgca acgctacgcg gcgcgcctcc ggcgcccgtg
     7561 cgcggatacg agtgtcggag cactcgtatc cgcgcacagc cctcgcggct ccgtcagctg
     7621 cccaggaacg ccagcacatc ggagcgggtc acgacacccg ccggcttgcc gtccacgagc
     7681 accagcgcgg cgtcggcggt ggcgaacgcg gtcatcgcgg tctccagtgc ctcgcccgca
     7741 ccgatcgtgg gcagcggagc agacatgtgc ttctccagcc ggtccgacag cgtcgcccgc
     7801 ccggtgaaca gcgcgtcgag caggtcgcgc tcgaccaccg cgccggccac ctccgccgcc
     7861 atcaccggcg gctccgcccg gacgaccggc atctgcgaca cgtggaactc gcggaggtag
     7921 ctcacggcat cggcgacggt ctcgttgggg tggacgtgca cgaggtcggg gatcgagcca
     7981 tccttgcggc gcagcacgtc acccacggtg gcgccctgcg ccggcggcag gaatccgtag
     8041 gtggccatcc aggagtcgtc gaagaccttg ccgaggtagc cgcgcccgcc gtccggcagc
     8101 aggacgacca ccacggcccg ctcggcctcg tcggccgaca gctcgcgcgc cacccgcagc
     8161 gcggcctctg cggccatccc gcaggagccg ccgaccagca gcgcctcctc gcgtgccagc
     8221 cggcgcgtca tgtggaacga gtcggcgtcg gagaccgcca cgatccggtc acagatgttc
     8281 ttgtcgtacg tggtcggcca gaagtcctcg ccgacgccct ccaccaggta gggccggcca
     8341 ccgcccccgc tgtacaccga tccctcgggg tcggcgccga tcacctggac gcggccgtcc
     8401 gagacctcct tcaggtagcg cccggtgccg ctgatggtgc cgccagtgcc gatgccggcc
     8461 acgaagtgcg tgatccggcc gtcggtctgc tcccagatct ccggacccgt accgaggtaa
     8521 tgcgactccg gattgttcac gttggcgtac tggttgggct tccaggcgcc ctcgatctcc
     8581 ctgaccaggc ggtcggagac ccggtagtag gagtccgggt gatccggctc gacggcggtg
     8641 ggacacacca cgacctgggc gccgtacgcg cgcagcacgt tgcgcttgtc ctcgctcacc
     8701 ttgtccgggc acacgaacac gcagctgtag cccttgcgtt gggcgatcat cgcgaggccg
     8761 acgccggtgt tgccggacgt cggctcgacg atcgtgccgc cacggcgcag ctcacccgac
     8821 gcctccgccg cctcgaccat ccgaagcgcg atgcggtcct tcacgctgcc accggggttg
     8881 aagtactcca ccttggcgag gacgggcgcg acgattccct ccgcaacggc gccgagccgg
     8941 acgagagggg tgttcccgac caggtcggcg acgtgctcga catagcgcat gcctccatgg
     9001 tcgcacccgg cggcagtcgc accgggcggc ggatccccgg cggcacaaca ggtcgagcac
     9061 ccacatggcg cgacgcaacg ggtgcatgtg tcaccgccgg tcgttgcgct ctcatcgatc
     9121 cggccgaacg gagcgcgaaa catggcgaac ggactagtct tcccagcacg gacaaagctc
     9181 atccgaccga gcgcatcggc cgggtgatct cacgacccaa gaccggcggg ccggggccca
     9241 cggtcccgcg ttcccgggac gaccgaccag caggaggggc gcaactgtgc catcggtcgg
     9301 cgacgacatg tcggaggtcg aggactccga cagggccgag gctgtgcgcc tggcccgtcg
     9361 cgacgccgag ctggacagcc tcgcgtcacg cgcagcgtcc ggacaccgtc cgtcgctgga
     9421 ccagttgctg cgctacgtcc ggccactggt tgtgcggtac tgccgggcgc ggatcgggag
     9481 cggggcgatc ggcctggcga ctcccgagga cgtcgcgcag gacgtactgc tcgggctgta
     9541 caacgccctc gggagatggc ggcccgacga gacgcgggtg atggcgttcg tctacggcat
     9601 cgcgagcaac aaggtcgtgg acgcgtaccg cgcggcgggg cgcaaccggt ccgtccccac
     9661 tgacatgctt cccgacgcgg ccgacaccgg tgcgggcccc gaggccatag ccgtgctcgg
     9721 cagccagatc ggtgagctgc gtcggctcct ggagcagctc ccggagcacc accgcgaggt
     9781 gctcgtgctg cggatagcgc tggggctgag cgcagaggaa accgcccgca ccatcgactc
     9841 cacggcgggc gccgtccgcg tgacccagca ccgggcactg acgaagctgc gccagctcgc
     9901 cgcgaaccgc ggctgaccgg caggtttcct cgacgctagc ggtacgcgcg cacgacctcg
     9961 gccagcctgc ggtcgag
//
LOCUS       DASRSC010000311         2784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3242194,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000311 DASRSC010000000
VERSION     DASRSC010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3242194"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..383)
                     /locus_tag="VFE39_15310"
     CDS             complement(216..383)
                     /locus_tag="VFE39_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Insertion element protein"
                     /protein_id="HET6260006.1"
                     /translation="MSERAVPFYCPYCGEEDLRPAEQTEKVPHGAWYCADCLRTFSLK
                     LVGIGVAEVSR"
     gene            complement(402..2072)
                     /locus_tag="VFE39_15315"
     CDS             complement(402..2072)
                     /locus_tag="VFE39_15315"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HET6260007.1"
                     /translation="MPPTTSTPGKRKRGEGQWKLGHREPLNPNERTKKDDDGLNVRAR
                     IENVYAKRGFDSIDPADLRGRMRWWGLYTQRRPGIDGGRTAVLEPEELDDRYFMLRVR
                     IDGGALTTEQLRAIGEVSQDHARDTADVTDRQNIQYHWIQIEDMPAIWKKLEGLGLLT
                     TEACGDTPRVILGSPVAGIAADEKIDPRPAIDAIIERHIGSPEFSNLPRKFKTAISWQ
                     QDVAHEVNDVSFVGVEHPEHGPGFDVWVGGGLSTNPKIAQRLGAWVPLDEVPDVWAGI
                     ISVFRDYGYRRLRHRARIKFLVADWGAAEFRRVLEEEYLGKKLVDGPAPATPERPIDH
                     IGVHKQVDGRNYIGVAPPAGRVSGSTLVALAKAAEAAGSRRVRLTPQQKIVVLDVPDR
                     NVKSLTAELAALGLQAEPSPWRRATMACTGIEFCKLAIVETKERAIRLVEELEDRLAD
                     VSPDVPISIHLNGCPNSCARTQVADIGLKGQIVTDANGNQVEGFQVHLGGGLGLDAGF
                     GRKLRGLKVTSAELGDYVDRVVRRFIDQREDGERFAQWVTRAAEDDLK"
     gene            2384..>2784
                     /locus_tag="VFE39_15320"
     CDS             2384..>2784
                     /locus_tag="VFE39_15320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260008.1"
                     /translation="MDDSVIDLGRRPGSTLRAIAVTLAILAVAAAIGAVDAGAGTGAR
                     IGAGVCAGLCAVGVVTLVRATRRTRRLVIDHAGIRLDLGARRKPAFRLAWTEISGVGL
                     LVVAVTAPHSFLLRLFGWPPPQVGTVLELVPA"
BASE COUNT          344 a          982 c         1001 g          457 t
ORIGIN      
        1 ggacccagct cggccgcacc acgctcggcg acagcgcgta agtcgatctc cgttgccaca
       61 ctcatcgttc tccctcgctg cgctcggtcg tccgcttcgc gggcacgctg tgtcgatggt
      121 tcgctccgca agctccgctc accgctcgtc ctcggtgcgc tcggccgacc gctgcgcgag
      181 ggcgctgtgt tgatggttcg ctccgcaagc tccgctcacc gcgacacctc cgcgactccg
      241 attccgacga gcttcaggga gaacgtccgc aggcagtcgg cgcagtacca ggcaccgtgg
      301 ggcaccttct cggtctgctc cgcgggccgc aggtcctcct ccccgcagta cgggcagtag
      361 aaggggactg cacgctcaga catctcggat cacctcgcgg gtcacttgag atcgtcctcg
      421 gcggcccgcg tgacccactg tgcgaagcgc tcgccgtcct cgcgctggtc gatgaagcgg
      481 cggacgaccc ggtcgacgta gtcgccgagc tcggcgctgg tcaccttgag tccgcgcagc
      541 ttccggccga acccggcgtc gaggccgagg ccgccgccga ggtgcacctg gaagccctcg
      601 acctggttgc cgttcgcgtc ggtcacgatc tggcccttga gcccgatgtc cgccacctgc
      661 gtgcgggcgc aggagttggg gcagccgttg aggtggatcg agatcgggac gtccgggctg
      721 acgtccgcga ggcggtcctc cagctcctcg accagccgga tcgcccgctc cttggtctcc
      781 acgatcgcga gcttgcagaa ctcgatgccg gtgcacgcca tcgtcgcgcg ccgccagggc
      841 gacggctcgg cctgcaggcc cagggcggcg agctcggcgg tcagcgactt gacgttccgg
      901 tcgggcacgt cgagcacgac gatcttctgc tgcggggtga gccgcacccg gcgcgagccg
      961 gccgcctccg cagccttggc cagtgccacc agcgtggatc ccgagacccg gccggcgggc
     1021 ggcgccacgc cgatgtagtt gcggccgtcc acctgcttgt gtacgccgat gtggtcgatc
     1081 gggcgctcgg gtgtggcagg cgcggggccg tccaccagct tcttgccgag gtactcctcc
     1141 tcgagcacgc gccggaactc cgcagcgccc cagtcggcga cgaggaactt gatccgcgca
     1201 cggtggcgca acctgcggta gccgtagtcg cggaacaccg agatgattcc cgcccagacg
     1261 tcgggcacct cgtcgagcgg cacccacgcc ccgagccgct gggcgatctt cgggttcgtg
     1321 gacaggccac cgccgaccca cacgtcgaag cccgggccgt gctccgggtg ctcgacgccg
     1381 acgaacgaga cgtcgttgac ctcgtgggcc acgtcctgct gccaggagat cgcggtcttg
     1441 aacttgcgtg gcaggttgga gaactcgggg ctgccgatgt gccgctcgat gatggcgtcg
     1501 atcgccggcc gggggtcgat cttctcgtcg gccgcgatcc cggcgaccgg cgagccgagg
     1561 atcacccggg gggtgtcgcc gcacgcctcg gtggtgagga ggccgaggcc ctcgagcttc
     1621 ttccagatcg cgggcatgtc ctcgatctgg atccagtggt actggatgtt ctgccggtcg
     1681 gtgacgtcgg cggtgtcgcg ggcgtggtcc tgcgagacct cgccgatcgc ccgtagctgc
     1741 tcggtggtga gcgctccacc gtcgatccgg acgcggagca tgaagtagcg gtcgtcgagc
     1801 tcctcgggct ccagcaccgc ggtgcggccg ccgtcgatcc cgggccggcg ctgggtgtag
     1861 aggccccacc agcgcatccg gccgcgcagg tcggcggggt cgatcgagtc gaacccgcgc
     1921 ttggcgtaga cgttctcgat acgcgcacgg acgttcagcc cgtcgtcgtc cttcttcgtg
     1981 cgttcgttcg ggttgagcgg ctcacggtgg cccagcttcc actggccttc gccgcgcttg
     2041 cgcttgccgg gagtggacgt ggtggggggc aaggcggtgc ctccgggtcg cgacgcgggc
     2101 acgccacggt cgagagcgcg gcaggcctcg gcgccgactg tggtggtggt cggggcgcga
     2161 gatcagcgct gtcgacagag cgcgctgctc acccggtgca ggtccacgtg gcggcgggcc
     2221 acgagtcgca gggtcagcgc ggtcacagag gacagagtgc cacttgtccg gtccacgatc
     2281 catcgacgtc cgcatgctgg gagcggactc acacgcccac tgcgtgcgac cggttctccg
     2341 aaaccgccca ccgcgggccg gatcgtgcgt acggtcccgc gacatggacg acagcgtgat
     2401 cgacttgggg cgacggcccg ggtcgaccct ccgggccatc gcggtgacgc ttgcgatcct
     2461 cgcggtcgcc gccgccatcg gtgcggtcga cgccggggcg gggacgggcg cgcggatcgg
     2521 cgccggtgtc tgtgccgggc tgtgcgcggt gggcgtcgtc acgctggtgc gcgccacccg
     2581 gcggacgcgg cggctggtga tcgaccacgc cggcatccgg ctcgatctcg gcgcccgccg
     2641 caagcccgct ttccggctcg cctggaccga gatctccggg gtcggcctgc tcgtggtggc
     2701 cgtgactgcg ccgcactcgt tcctcctgcg gctgttcggc tggccgccgc cccaggtcgg
     2761 gacggtgctg gagctggtcc cggc
//
LOCUS       DASRSC010000312         7081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_127717,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000312 DASRSC010000000
VERSION     DASRSC010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7081
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127717"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(614..686)
                     /locus_tag="VFE39_15325"
     tRNA            complement(614..686)
                     /locus_tag="VFE39_15325"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Lys,seq:ttt)
     gene            815..1390
                     /locus_tag="VFE39_15330"
     CDS             815..1390
                     /locus_tag="VFE39_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015788011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET6260009.1"
                     /translation="MRSRYPVLAGLIGVLVLPGCTPATVALQPSRSMVRNVEVSATFT
                     EALSTAVTYDRRLVKAGSHAAVSSRSEGGITTVTLVVRGFEPERRYGAHVHSDSCGEK
                     GADAGPHFQNVVDPVQPSVDPRFANPQNEIWLDLRTDETGAGTGETTVAWQIPPDRRP
                     KSVVIHARPTGPGPGVAGTAGARAACVAVEF"
     gene            1412..2554
                     /gene="dusB"
                     /locus_tag="VFE39_15335"
     CDS             1412..2554
                     /gene="dusB"
                     /locus_tag="VFE39_15335"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019806689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET6260010.1"
                     /translation="MSAPTPVAAKPLRIGPYASDTPVVLAPMAGITNAGFRRLCREQG
                     AGFYVCEMITSRGLVERNPLTFRMIAMDPDEHPRSMQLYGVDPATVGAAVRMVAERDL
                     ADHLDLNFGCPVPKVTRRGGGAALPYKRRLFEAIVRAAVDNAGRIPVTVKMRIGIDDE
                     RRTYLDAGRRAADAGVAAVALHARTAAQRYSGTADWSAIARLRDALPAGLPVLGNGDI
                     FCAADALAMVAATGCDGVVVGRGCLGRPWLFGDLEAAFAGRPLPVPPTLGQVAATMRR
                     HAVLLQEHMGADKGIRDMRKHIAWYLKGFPVGSDLRRKLGLVGSLDELDALLAQLDAH
                     QEFPAEADGPRGRQGSPGRVVLPEHWLDDPDDASVPLGAELEHSGG"
     gene            2714..3538
                     /locus_tag="VFE39_15340"
     CDS             2714..3538
                     /locus_tag="VFE39_15340"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018687078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6260011.1"
                     /translation="MDTRDGVPPREASRPSEHSATPPATANAAARHTALAEELAGAGR
                     WEQAYQHLRTAVRLLHGQPVPDELDRLRREHAEAREQSLRDSLTASYNRRYLDERLVA
                     LLDDPLCRGTAGISVALVDVDHFKQVNDTYGHPFGDRVLQRLVTELDAGLPDSAFCAR
                     YGGEEFALVLPGHDLVDAVRICEAARHRIDRHPWHELDPGLRVTVSIGVAHTSGPVTG
                     LERLVGMADALLYGAKHSGRNAVAYQDERNGSVRLAGPAGGRRSIRWRRLRTDTRL"
     gene            4832..6508
                     /locus_tag="VFE39_15345"
     CDS             4832..6508
                     /locus_tag="VFE39_15345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET6260012.1"
                     /translation="MGAPLHAQTAVGNAEATTRVRAAESDSAAGSSRALDRSEQAERT
                     RRTERESGTGNRASHIDETLTRLTAAHAGLTLPRRDGGDEPELPPPAPKRRPRLTAGR
                     LLAGALALLVFATTTFGWGAEGWLESSIRNAAALDPQSGSIVDAAAQLGDENVLIVAT
                     DAGSAPGAGAGTAGGTGASADSVTVAHIPAGGGPLVVLSVPDGLEINRPPCERWDPAA
                     ASYLDQSVPAEARTQLRSALDVGGPRCVTRVVQQLTGLAITKYVGIDLDALSAMADAV
                     GGATVCVTKPVVDGALGPVVPDPGATTLSGVRAADFARATDVQGDPPSDYGRIERQQQ
                     LLAAVLDKAVSGTALLDVAQLVALRPALGRAIVTDGAGLDEVLAVATSLSRLDANGVT
                     FAAVPTAGDANNPGQTVLRDADAAQLFTALRQGSPLPEQARDLRAADSGPVPADVKVE
                     VLNASARAGLAGQVGETLSSLGFGVGQVGNADQPTPQTVIRFSPDEAAAAALLATTVP
                     SATSVPDPGTTGVLQLVLGKSFDDVVRAPAEPIALQADGPSVTPTTATCP"
     gene            6583..6933
                     /locus_tag="VFE39_15350"
                     /pseudo
     CDS             6583..6933
                     /locus_tag="VFE39_15350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026074.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhoU domain-containing protein"
BASE COUNT         1021 a         2690 c         2440 g          930 t
ORIGIN      
        1 gcgggcaaca ccgacctggg tccgtcgatc ggctacgtct tcgccgcgct cgcgctgttc
       61 gccgcggcgg caggcgccac ctggagcgtg gacgccgtga tccgtccgca cctgggacgc
      121 ttcgccccgc tggccggctc cttaccggca gtgtgacgac gatctcaggt aaggcttcgg
      181 ccaacctggc ctgagccgca cctgcacctg caccggtgct gacgctgacg tgctggacgg
      241 acccgagcag cgggtgaacg acaccgggtt cgatgcggag cggcagaacc gggcttcccc
      301 gtggccgggg gatcccgacc ttggccgcgc acacgcagag gagaagtagg caggagtcac
      361 caggcacctg gagcgtggtg ccaccaggcc gctgaccagc atcgcggctg gcgtgcggat
      421 gaaggtggtg caggagacgt tggggctcgt cgagtgcgat cgccgtcttc cccaacggtc
      481 gctggggcgg ccgcggaggc cgccccagcg atggcgccga gggcggcagc gggaacggct
      541 acgcacacac cgcgcacaca cccttcgtcg gcgggtcctg ctagtcctgc tagtccgaag
      601 aacagaatgt gggtgggccc ccgggggatc gaaccccgaa cccgcggatt aaaagtccgc
      661 tgctctgcct gttgagctag aggccccggt gtcgccccgt aggccgggcg acgcagtacc
      721 accctacggc tcccgtctcc ggtcgtgctc gtcactctgt cgtggctgcg gaggccggtc
      781 gtcgctacgg gatgtcaagc tccatcctca agccatgcgc tcccgatacc cggttctcgc
      841 cggactgatc ggcgtactgg tgttgcccgg ttgtaccccg gccaccgtcg cactccagcc
      901 gtcccggtcg atggtgcgca acgtcgaggt cagtgccacg ttcaccgaag cgctcagcac
      961 ggcggtcacc tacgaccgga ggcttgtcaa ggcgggttcg cacgccgcgg tgagttcccg
     1021 gtccgagggc gggatcacca ctgtcaccct ggtggtgcgg gggttcgagc ccgagcggcg
     1081 atacggcgct cacgtacaca gcgattcgtg cggcgagaag ggcgcggacg ctggtccgca
     1141 cttccagaac gtggttgatc cggtgcagcc gagtgtggac ccgcggttcg ccaacccgca
     1201 gaacgagatc tggctcgatc tcaggacgga cgagacgggc gccggtaccg gcgaaaccac
     1261 ggtggcctgg cagatcccgc ccgaccggcg gccgaagtcg gtggtcatcc acgccaggcc
     1321 gacggggccc gggcccggtg tggccggtac ggcgggcgcc cgggccgcct gcgtcgccgt
     1381 cgagttctag cggcgaggac aatagggcat cgtgagtgcc ccgacccccg tcgccgcgaa
     1441 gccgctgcgc atcggcccgt acgcgtccga cacacctgtt gtgctggcgc ccatggccgg
     1501 catcacgaac gcgggtttcc ggcgcctgtg tcgcgagcag ggcgccggct tctacgtctg
     1561 cgagatgatc acctcccgcg gcctcgtgga acgcaacccg ctgaccttcc ggatgatcgc
     1621 gatggatccc gacgagcacc cgcggtcgat gcagctctac ggagtggacc ccgcgacggt
     1681 cggggctgcg gtgcgcatgg tggccgagcg ggatctcgcc gaccacctcg acctcaactt
     1741 cggctgccct gtcccgaagg tcacccgacg cggtggcggg gcagcactgc cgtacaaacg
     1801 gcgcctgttc gaggctatcg tccgggccgc tgtggacaac gcgggtcgga tcccggtgac
     1861 cgtcaagatg cgcatcggca tcgacgacga gcgccgcacg tacctcgacg ccggacgccg
     1921 ggcggccgac gccggcgttg cggccgtcgc gttgcacgcc cgaacggccg cgcagcgcta
     1981 ctccggcaca gccgactggt cagcgatcgc ccgactgcgt gacgccctgc ctgccgggct
     2041 accggtgctc ggcaacggcg acatcttctg cgccgccgac gcactcgcaa tggtggcggc
     2101 caccggctgc gacggggtgg tcgtcgggcg cggctgcctc ggccgtccct ggttgttcgg
     2161 tgacctggaa gctgcattcg ccggccgccc gctgccggtc ccaccgacgc tcgggcaggt
     2221 ggcagccacc atgcgccgcc acgccgtcct gctgcaggaa cacatgggcg ccgacaaggg
     2281 cattcgcgac atgcgcaagc acatcgcctg gtacctgaag ggcttcccgg tcggttccga
     2341 tctgcgccgc aagctcggcc tggtcggcag cctcgacgag ctcgacgcgc tgctcgcaca
     2401 gctcgacgcg caccaggagt tccccgccga agccgacgga cctcggggac gccaggggtc
     2461 gccggggcgt gtggtgctgc ccgaacactg gctcgacgac cccgacgacg cgtcggttcc
     2521 cctcggtgcc gaactggagc actccggcgg ttgatgcacc gacgggtaat gacatgatca
     2581 cgcacggcta gcgtcccggt cgggtggatg ctcccgcgaa cgggtgcgat caactcaagg
     2641 gtcactaccg tcccgtcgac accactggca acaggggagg tgagtggtgg ttacggtgcg
     2701 cgcggctgta cccatggaca cgcgggacgg ggttccgccc cgggaagcgt cacggccgag
     2761 tgagcactct gcaacaccgc ccgccacggc caatgcggcg gcccggcaca ccgccctcgc
     2821 cgaggagctc gccggagccg gccggtggga gcaggcctac cagcacctgc ggaccgcggt
     2881 tcggctcctg cacggccagc ccgtccccga cgagctcgac cggcttcgcc gcgagcacgc
     2941 cgaggcacgt gagcagagcc tccgggactc cctcactgcg agctacaacc ggcgctacct
     3001 cgacgagcgg ctggtcgcgc tgctcgacga tccgttgtgc aggggcacgg ccgggatatc
     3061 cgtcgcgctc gtggacgtcg accacttcaa gcaggtcaac gatacctacg gccacccgtt
     3121 cggcgatcgt gtgctgcagc ggctcgtcac cgagctcgac gccgggctgc ccgacagcgc
     3181 gttctgcgcc cgctacgggg gcgaggagtt cgcgctggtg ctgcccgggc acgacctggt
     3241 ggacgccgtg cgaatctgcg aggcggcgcg ccaccgcatc gaccgtcacc catggcacga
     3301 gctggatcct gggctacggg tgacggtgag catcggggtg gcgcacacgt cggggcccgt
     3361 caccgggctg gagcggctcg tcgggatggc ggacgcgctg ctgtacggcg cgaagcactc
     3421 cggccgcaac gccgtcgcgt accaggacga gcgcaatgga tcggtgcgcc tcgcgggacc
     3481 agccggcggc cggcgctcga tccgttggcg ccgtttgcgt accgacaccc gcttgtgatg
     3541 gaaacgatcc cctccgtaag ctcggcttgg agtcagaggg ggcgggaccg ggtcaccacc
     3601 cgtgcccgcc ccttgctcgt gccaaggagg ttgcgtggag gacggtccga accgtgctcg
     3661 gcgctccacc gcacccggcg gccgcagacg ggcccccgag gcgcccgacg acgttcttac
     3721 ccggcatggc ctgctcggcg tcccatccca cgaggatgtc ccccccgccc cgacaggggg
     3781 ccgggccgcg cggcgacgcg ctgccgagga acgcgaggca gctgcacgtc cgccggttga
     3841 cgatcctcgt gccgccgcgc cgttcgctcg gggtgcgaga ttcgacgccg ctgtgccgtc
     3901 cccgcaggtt cccacgcgcg acccgggtcg ctcggcccgc ggagccggcc ctgcccgatc
     3961 ggccgccggc cccatccgac cggcctccga ccatgggtat ccggctccgc cggtcaccgc
     4021 gacgcctcgc gtgccccgcc ggcccgcccc cgaccccgac ccgtggcggg ccggcccgcc
     4081 acgcgagtcg ggccgccact cgtccccgac cggcgtcccc ccgcgcgcca cacccccgcc
     4141 cctgcgcggt gggccggcct ccgacccagg acgcgatgcc ccctggacgg ctgtcccgcg
     4201 caatccggag cgagacacac gggcgccgac gcgccaccgc cccgctgtcg attccgacgg
     4261 acccacacgg gggcgccacg atggcgggcc gcaggagaaa ggtcgacggg cggcgaaccc
     4321 gaccatgcca gccggcggac ctgcacccgg agcgcagccc gagccagggc gccattcgtc
     4381 gtcccgcccc tcccgcgcag cggccccgga cctcggtgcc gggcgcgcgg caaccccgcc
     4441 cccgcccccg ccctcgcgat ccgagcggtc gggtccgcac ggtcaggtag cgccggacgc
     4501 gtcggcgtcg cgcatgtccg gccgccgccc ggcaggctcc atcgcggcca actccgagcg
     4561 cgccacctcc cggacccagt ccggcgcgcc gtcgcgtgag tcagcggcac cagaggcacc
     4621 ggcccgcgcg ggcggccgag cctcccgtcc acgccgcgag ccggacgccg cccccgccgc
     4681 ccctgccacc tccggacgcc gccgagccca acccaccacc ctcaccaccg atcaaccgac
     4741 cacacgcagc cgaaccacca ccccacgcca cgaccccgac gcagccatct ccacctccgg
     4801 acgccgccga gcccaagcgc ccacatccaa tgtgggcgcc ccgctacacg cccagacggc
     4861 agtcggcaac gccgaagcga cgacccgggt ccgcgccgca gaatccgact ccgcggcggg
     4921 ctccagccgg gcactggacc gcagcgagca ggccgagcgc acgcgccgga cggagcgtga
     4981 gtccggcacg ggaaaccggg cctcacacat tgacgaaacc ctgacccggc tcaccgccgc
     5041 ccatgccggc ctcaccctgc cgaggcgcga cggcggggac gaacccgagc tgccgccccc
     5101 tgcaccgaaa cggcgccccc gcctcaccgc gggccggctg ctcgccggcg cgctcgccct
     5161 gctggtgttc gcgacgacga cgttcggctg gggcgcggag ggttggctgg agtccagcat
     5221 ccgcaacgcg gcggcgctcg acccgcagtc cggctcgatc gtcgacgccg ccgcccagct
     5281 cggcgacgag aacgtgctga tcgtcgcgac cgacgccggc agcgccccag gcgccggggc
     5341 cgggactgcc ggcggcaccg gagccagcgc ggacagcgtc accgtggccc acatcccggc
     5401 cggtggcggc cccctcgtcg tgttgtccgt ccccgacggc ctggagatca accggcctcc
     5461 gtgcgagcgg tgggatcccg ccgcggcgtc ctatctcgac cagagcgtgc cggccgaggc
     5521 cagaacgcag ctgcgctcgg cgctcgacgt cggggggccc cgttgcgtca cgagggtcgt
     5581 tcagcagctg accgggctcg cgatcacgaa gtacgtcggg atcgacctgg acgccctcag
     5641 cgcgatggcg gacgcggtgg ggggcgcgac cgtctgtgtc acgaagccgg tggtggacgg
     5701 tgcgctcggc ccggtcgtgc ccgatccggg tgccaccacc ctctcgggcg tgcgcgccgc
     5761 cgacttcgcg cgggccacgg acgtgcaggg tgacccaccg tcggactacg gacggatcga
     5821 gcgccagcag cagctgctcg ccgcggtcct ggacaaggcg gtctccggaa ccgcgctgct
     5881 cgacgttgcg caactcgtcg ccctgcgccc cgcgctcggc cgagccatcg tgaccgacgg
     5941 cgccgggctc gacgaggtgc ttgcggtggc gacgtccctg agcaggctcg acgccaacgg
     6001 ggtcacgttc gcggcggtgc ccacggcggg tgacgccaac aacccgggtc agacggtgct
     6061 ccgcgacgcc gatgcggcgc agctgttcac tgccctgcgg cagggctccc cgctgcccga
     6121 gcaggcccgc gacctccgtg ccgccgacag cggacccgta ccggccgatg tcaaggtcga
     6181 ggtcctcaac gcatcggccc gggccgggct cgctggtcag gtcggtgaga cgctgagctc
     6241 cctggggttc ggggtcggcc aggtcggcaa cgccgatcaa ccgacgccgc agaccgtcat
     6301 ccgcttctcc cccgacgagg ccgcggccgc cgcgctgctc gcgaccaccg ttccctcggc
     6361 gacctcggtg cccgatccgg gtaccaccgg ggtgctgcag ctcgtgctgg gcaagtcgtt
     6421 cgatgacgtc gtccgcgcac cggccgaacc catcgccctc caggccgacg gaccgtccgt
     6481 caccccgact accgcaacat gtccgtgacc cggtgagcga cgttcaccag cggttcaccc
     6541 ggtcatcagg ctaaatgtcc ggctcacctc tactctcgac gcatgcgtga cgcctaccag
     6601 gagcaactcg acgaattggc cgatgccctg gccggcatgt gcaccgaagt ggcggaggcc
     6661 atgggaaagg ccacccgctc gttactggag tgcgacctgc acctcgccga ggaggtcatc
     6721 tccgaggacg tccgggtcga cgagatccgc gcagcggccg aggagagggc gttcgcgctg
     6781 ctcgcgctgc aggctccggt agccaccgat ctccggatcg tcgtctcggc gatccatggg
     6841 gcgggtgaca tcgagcgcat gggcgacctg gcgctgcacg tggcccagac ggcgcgccgc
     6901 cgccacccgc agccagtgct tcccgacgag gtctgacggt cgtcgccacg gcctgctcga
     6961 accccaccag agccccgaca ccgtcatcgt tgccggacgc catcaccgcg atcatgaaca
     7021 agccccgcta cgccacggcg agctggagcc tgctggccgc cgatatcgcc acggggaaga
     7081 c
//
LOCUS       DASRSC010000313        18686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1768318,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000313 DASRSC010000000
VERSION     DASRSC010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 18686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18686
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1768318"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..282)
                     /locus_tag="VFE39_15355"
     CDS             complement(28..282)
                     /locus_tag="VFE39_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260013.1"
                     /translation="MDDSINVNESVETFNPWTVVNLVFHHLADHGLHPVLGEAGDPAV
                     PAAALLRSFGIEPGRNDESVTSPAVQHELAQIRARMFGEP"
     gene            428..1486
                     /locus_tag="VFE39_15360"
     CDS             428..1486
                     /locus_tag="VFE39_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET6260014.1"
                     /translation="MSSARAERLVNLVLCLLSTRQFLPAERIRQIVPGYADARGDDAF
                     FRMFERDKNELRELGVPLETGRRSSFDTVDGYRIARQDYELGDIELEPDEAAAVALAA
                     RLWDSPDLAVPAHSALVKLRAAGVEVDEDQGRVQPRVRTTEPAFAPLLAAVRAARAVT
                     FEHRRGGPGGVVQRRTVEPWGVVSWRGRWYLAGHDRDRDAPRSFRVSRISEAVTPIGP
                     AGAVTVPADVDLMAMVRRNVDPPAVTGTARVWVADSRAHGLRRLARAVGRYTHGGRPG
                     DELELDLRNVETVARWLAGHGPDVAVLGPESLAAAVRANWAAAAAAHATRDLHADGRF
                     PVSDHHVVVRPGRAGIGP"
     gene            1483..2472
                     /locus_tag="VFE39_15365"
     CDS             1483..2472
                     /locus_tag="VFE39_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HET6260015.1"
                     /translation="MSTGGVTERLPRLLSLVPYLLARPGIPVAEAAADFGVSERQLRR
                     DLELLWMCGLPGYGPGDLVDLSFSGDTVTVTHDAGMRRPLRLTTAEATALLVALRTLA
                     DVPGVVDTAAVQRATAKIERAVGDAGLVGVAVDLSREEAATTAAVREALAVGRALRIV
                     YYTAGRDAVSQRTIDPMRLLIVEGRGYLEAWCRRVEAVRMFRLDRVEEVEVLDEPAAP
                     PAEAEPTDVSEGLFQPSAEHRFAVLLLEREARWVAEYYPVDDVVELDGNRARVLLRYA
                     DPAWLVRLVLGLGGRAQLLEPPELVDAVGSRAREALAAAGGHGDRDTVEQSGG"
     gene            2476..2664
                     /locus_tag="VFE39_15370"
     CDS             2476..2664
                     /locus_tag="VFE39_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260016.1"
                     /translation="MGEFLPALVTAAVGLILLVLVLLLVLRSVHRVRRASAALRLDTG
                     ERATSLHALVNSRRRSHE"
     gene            2762..3067
                     /gene="tatA"
                     /locus_tag="VFE39_15375"
     CDS             2762..3067
                     /gene="tatA"
                     /locus_tag="VFE39_15375"
                     /inference="COORDINATES: protein motif:HMM:NF001854.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HET6260017.1"
                     /translation="MPSLGGWEFVILIGILVLLFGAKRLPDMARSIGQSARVFKGEMK
                     GLKGEEAPAGGDAARAEKPADASAPPAALPATQPAQVQETKAEPTRATQPGSSSTGQ"
     gene            3077..4078
                     /gene="tatC"
                     /locus_tag="VFE39_15380"
     CDS             3077..4078
                     /gene="tatC"
                     /locus_tag="VFE39_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HET6260018.1"
                     /translation="MARLSLRRGGRRPKNPDGTMSLVEHLYELRNRLTIALIAIALTT
                     IIGYIWYGTGFFGLPSLGNLLKEPYCAIPATSRGQFSSDPNACTLLATGPFDQFNLRL
                     KVAMTAGIVLACPVWLYQLWGFITPGLYAKERRYALSFVSVAAVLFVFGAVLAYLVVS
                     QGLSFLLSIGDNVQTTALTGEYYFSFIIALLLIFGVSFEIPLLVVMLNLAGVISYAKL
                     KAWRRGLIFGLFVFAAIATPGQDPFSMLALALALVLLFEIAIQITRVNDRRRAARRAA
                     EGWDEWDPDAPSPIDTSPSQIDTTPSQVDTTPSPIESPSPIETLPASDAARRVDDAT"
     gene            complement(4084..4425)
                     /locus_tag="VFE39_15385"
     CDS             complement(4084..4425)
                     /locus_tag="VFE39_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HET6260019.1"
                     /translation="MTTYAVAHMREVTVGPAIIEYLQRIDETLEPFGGRFIVHGAEPT
                     VLEGDFPGRLIVIAFPDREQAQGWYDSPAYRAILPLRTENSLAEAVLVDGVGDDHRAT
                     DVLAPDMRAHR"
     gene            complement(4530..5021)
                     /locus_tag="VFE39_15390"
     CDS             complement(4530..5021)
                     /locus_tag="VFE39_15390"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HET6260020.1"
                     /translation="MPSTLPDIAPAFVQMAHRIVWATAATVDERGRPWTRVLHPIWQW
                     DGERLTGWIATGPTPAKRAHLAAHPYVSLTYWDPAQDTASVHCRASLHTDDETRTEVW
                     ERFRTGPEPVGYDPAIIPAWDGPLSPGFAALRLEPWMLRVQPAAVLLEGAGDQIMVWR
                     EET"
     gene            5089..7863
                     /locus_tag="VFE39_15395"
     CDS             5089..7863
                     /locus_tag="VFE39_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET6260021.1"
                     /translation="MWKPGSVASSNPAPAEAYAAARSRAARPRLAEFATDLPFELDGF
                     QHTACSALEDGHGVLVCAPTGAGKTVVGEFAVHLALAQGLKCFYTTPIKALSNQKYAD
                     LVERHGAEAVGLLTGDTVVNGDAQVVVMTTEVLRNMIYAGSRQLEQLGFVVMDEVHYL
                     ADRFRGAVWEEVILQLPEHVALVSLSATVSNAEEFGDWLVTVRGDTTVVVDEHRPVPL
                     WQHMMVGSRLLDLFAGEDAAGELQVDPDLVRYTRDLDRQSAVWDRGRGRGSRGGGNGR
                     SSTRFRPPSRTTVIDRLDRNGLLPAITFVFSRAGCDAAVGQCVRSGLRLTTEEEALEI
                     RRTVERRTADLPQGDLAVLGYWEWLEALERGIAAHHAGMLPAFKETVEELFVRGLVRA
                     VFATETLALGINMPARTVVLERLVKFNGEAHAELTPGEYTQLTGRAGRRGIDVEGHAV
                     VVWQPGVDPVQVAGLASRRTYPLRSSFRPGYNMAVNLVGRLGTEAARELLEQSFGQFQ
                     ADRSVVGLARRIERNTEALAGYDESMHCHLGDFAEYAALRRQVGEREKALSRQGVEDS
                     REAAADSLRSLRPGDVIAVPGGRRAGLAVVLDPGLDKDADPRPLVLSEDRWAGRLSPA
                     DFPTPVEPLGRMRLPRRVDHRTPSVRRDLASSLRNTGIVAPATPRRRSRGGNVDDPEL
                     ATMRRALRAHPCHGCDDREAHARWADRYARLERETEQLRQKVRATTHSLARAFDRIRS
                     LLGERGYLDGDEITEHGERLARLWGESDLLTAECLRHGIWEGLEPPELAAVVSALVYE
                     SRRDNGQMPRVPSGAVSEALTSTVRLWAELEGDERRHRVDRTREPDLGFAWPIHRWAR
                     GESLSVVLTAAEQNGAELSAGDFVRWCRQVLDLLDQIAGVMGRSSGVGRAAAQAGTAI
                     RRGVVAVGTV"
     gene            7935..8846
                     /locus_tag="VFE39_15400"
     CDS             7935..8846
                     /locus_tag="VFE39_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016334188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HET6260022.1"
                     /translation="MSTPQGPNPPNGTPHGQPAEQPAWGQQPPGGWGQQGEQGWDPDE
                     TRGVPTHGGPGAAQPGYADQNPNGNSQQQYGSQTQDLPQYGPPPQQGLPQYGHPQQGP
                     PPYGQAPQQGQPQNPSQYDQPTQQGQPAYGQQPYGWPQQSQPQYGQTGPGQPRGAEWP
                     GQQQGWGQYAPAQQWPGQPQGTQQWGQGPEPAKPSRSGKKKFSSSLIIGGAVVVVAVI
                     AVLGFVAPGFFVTRVFDTAALQDGVQRVLTQDYGLANVAGVRCSEGIRVEQGATFQCD
                     ATVDAAPVKVPVTITGSDGAYEVGRPG"
     gene            8906..10123
                     /locus_tag="VFE39_15405"
     CDS             8906..10123
                     /locus_tag="VFE39_15405"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6260023.1"
                     /translation="MTHDVRTLDPDQLRSAHILFGSTIHRGPVDDEWWSRAATSYSPG
                     RTVGIHDGGALVGTAMAFPSRLVVPGGGVLPMAAVTRVGVRADCTRRGLLSAMMRAQL
                     DDVAARGEPVATLRASETRIYGRFGYGVATRGRDLRVRVGRPAWRPDAAQGGSVRLLD
                     RDDIIPTLREVHEGIALTRAGSMTRQDSWWWGAIGRRVEDREPVIAAVHTGAHGDDGF
                     AVAFRTDADSFSEQTLEVADLHAADMEATAGLWRFLLDIDLVSSVQAELRPLDEPLEL
                     LLSDPRACAVTGHHDETWLRLVDVPTALAARSYGAAEPVLLAVHDPLLEKNAGVYRIA
                     GGGAERVAPLGSPMQPQLECDVAALAMAYLGDRSPSQLVATGWWRAHDGAAVHRADAA
                     FTTSVTPWCGTHF"
     gene            complement(10137..11072)
                     /locus_tag="VFE39_15410"
     CDS             complement(10137..11072)
                     /locus_tag="VFE39_15410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HET6260024.1"
                     /translation="MLLDAASLYFRAYYGVPESMTAPDGTPVNAVRGFTDMVARLITD
                     RRPTRLVACLDLDWRPAFRVEALPSYKAHRVPGEPEVAPAGLPEEVPDTLAPQVPVLL
                     EVLAAAGIATAGAEGYEADDVIGTLAAAEREDPVLAVSGDRDIMQIVRDEPVPVRLLY
                     VGRGLAKAEHLGPVEVAEKYGVPAARAGEAYAEMAMLRGDPSDGLPGVPGVGAKTAAT
                     LVGRFGSWAELRAAVTDRSDVRMSPPVRTKLAAAAPYLAVVEPVVRVALDAPVQLDRP
                     DKKPSSPADPDRLAALAERWGVGSSIDRLLTALAR"
     gene            11130..12257
                     /locus_tag="VFE39_15415"
     CDS             11130..12257
                     /locus_tag="VFE39_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HET6260025.1"
                     /translation="MTPAVPSALFADRLRRAGEVAAVHDTDLLLVTPGTDLRYLIGAE
                     GSSHERLTCLVLPAAGHRAPPALVVPRLEAPGLAGLPLEELGVEIVTWTDGDDPYLLV
                     SDLAGGPTRIRLVDSMPAAHVFGLRDAFPEVTQELAGPVIRELRMRKDTAEVAELRAA
                     GAAIDRVHARMGEFLKPGRTEKQVGADIAAAIIEEGHTQAAFVIVGSGPNGASPHHGV
                     SDRVIESGDVVVIDIGGPLPSGYNSDCTRTYAVGEEPSASVREAYAVLQDAQERAVAA
                     ARPSVTAEQVDAAAREPIMAAGLGERFVHRTGHGIGLDVHEEPYIVGGNELQLEPGMA
                     FSVEPGVYLDGEWGARIEDIVVVTDDGCERLNTRPRDLAVL"
     gene            complement(12408..13208)
                     /locus_tag="VFE39_15420"
     CDS             complement(12408..13208)
                     /locus_tag="VFE39_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004560592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6260026.1"
                     /translation="MPEPVRRGLGDFLRSRRERLTPEVVGLPTRRKRRTPGLRREEVA
                     ELAGIGVDWYVRLEQGRTVSPSTTTIDALARALRLDDTERIHLRALAGNPAKAAFVRE
                     RVPGAIRHLVESLSQPAYITGRRWDLLAWNAAAVDLFTDFGRVTDEDRNVLVYILLDP
                     DARRLFGDGWADQAAHVVAQFRATHDLWAPDPAFEELSARLRHGCPEFHRWWGQHDVL
                     GSGAGQKILHHPAKGTLCFEYATFQANDDPALRLTIYTPADSTPPVRP"
     gene            13335..14414
                     /locus_tag="VFE39_15425"
     CDS             13335..14414
                     /locus_tag="VFE39_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020659859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET6260027.1"
                     /translation="MKAAVLSAFGSPLSVESVPDPTLGTGEVIVDVVAAGVLPYAADV
                     LSGKRRHLLTLPVAPGAGAVGRVRAVGPDATRLAAGDWVLCDPTVRSRDDPLTPDITL
                     QGLSARGDGGLRLQRHFGQGSFAEQLLVPTENAFPLGSVDLAHAGRWTALNLCLVPYG
                     GLLAADLKAGETVLVSGATGNFGSAGVAVALAMGAGCVVAPGRNQDMLTELERRFGSR
                     LRTVSLSGDEDQDRERMTQAAPGPIDVVLDLLPPSAGTTPVRAAAMTVREYGRVVLMG
                     GVGMLGGDDLALPYPWIMRNSITVRGQWMYPPQANTRLIELARSGLLDLGQFDVTEFA
                     LDDAAKAIAHAADSNGRFTLTVIRP"
     gene            complement(14431..15474)
                     /locus_tag="VFE39_15430"
     CDS             complement(14431..15474)
                     /locus_tag="VFE39_15430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HET6260028.1"
                     /translation="MRSRSLLAMLAVAAAAAPLGLLGAPAAAAAARPTAVVAMGDSAA
                     SGEGAGDYESGTRGEGGDWCHRSAHAYVRRTGLAAEAVNLACSGAAAADVAFGPGTHN
                     TEGSQAQRLVDVAARYQVTTVVLQVGANDDAALTGTGIACIRAFLNPREPPCRGTLGP
                     LVADRMGATAAKVERAVRDVQEAMRRAGYADGSYELVLASYASPVTERMVPVQGLQGC
                     PYSKADAGWGRTALFPALSAALGGVAQRTGTRFLDMTRATEGFEACSRALNSQEWQRR
                     ITVDPQAFVHGGLDAAGYHLAQESFHPTAGAHAEMGRCVGDFVRSGAPAAACVAGADG
                     RTHLEGTVLAPAA"
     gene            15724..15912
                     /locus_tag="VFE39_15435"
     CDS             15724..15912
                     /locus_tag="VFE39_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET6260029.1"
                     /translation="MASTLTRPRHGKVIAGVCAGLAERFGWSPFVVRLAFLLSCLLPG
                     PQFVIYLVLWVIMPKRPY"
     gene            15938..17482
                     /locus_tag="VFE39_15440"
     CDS             15938..17482
                     /locus_tag="VFE39_15440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6260030.1"
                     /translation="MTRILLLGARVLDPGGPAGPDAVEISDGRITFVGRVAHARERMR
                     GAAVLELDGAVLTPAFVDAHVHATATGLLVDGLDLTGCASPEALLDAVAARAAARPGA
                     LVWGHGWQDQHWPQPPPPRPALDRAAAGAPVYLSRIDVHSALVSSALLDRAPAAVGAP
                     GWSDGPLSADAHHLARRAALTAVDGVARAAAQSAFLTAAAARGVAVVHECAGPDISSA
                     ADLAELLARPGPEVVGYWGEAVTTPGQVRELLATTGACGLAGDLFVDGSIGSRSAALR
                     APYADAEGCTGNRYLDADAVAAHVAACTAAGVQAGFHVIGDAAADVLLAGLDTAATAT
                     SAAAVQAAAHRVEHLEMLDPAQARRLAQLGVVASVQPAFDALWGGRAGMYAERLGAGR
                     AARMNPFAALRDAGVVLALGSDTPVTPLDPWAAVHAAVHHQTPGSGLTPNEAMEAHTV
                     GGYRAAGDRSPLAGRLVPGAPASYVVWDGAGPLAGDPHPPRCLRTVHRGVILHDALRD
                     VGTFPP"
     gene            17613..18413
                     /locus_tag="VFE39_15445"
     CDS             17613..18413
                     /locus_tag="VFE39_15445"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET6260031.1"
                     /translation="MTRSPDLRGQTVLVLHAHPDDEAIFTGITLSRLAEAGARTVLVV
                     ATAGELGQSRLPLRPGETIPERRVAELERAAGLLGVSRLVLLGRRDSGLPGWSDGQHA
                     RALMAADPLRLARHVAEIADDEGAGTLVHDDEHGIYGHPDHRAAFRIGATAAELVGAT
                     GYRVTVDREHLHITARDQHLVHGAARAAAVPFGRVTAEIGLAVSGSPGHLGRKRAAMI
                     SHSSQIAPEDVPDAGFASAYGIEWYRRSGEPGLLDELGNVHLLAGAPA"
     gene            18500..>18686
                     /locus_tag="VFE39_15450"
     CDS             18500..>18686
                     /locus_tag="VFE39_15450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HET6260032.1"
                     /translation="MAAPTASPVASDPGPPPAARRRRPWWAAFAARLVGSAGGGLLLY
                     LSFPPRAQWWLALPAFGL"
BASE COUNT         2511 a         6680 c         6845 g         2650 t
ORIGIN      
        1 actcgtatcc gcgcacgacc ctcgacgcta cggctcgccg aacatccgcg cgcggatctg
       61 ggcgagctcg tgctgcaccg ccggggaggt cacgctctcg tcgttgcgcc ccggctcgat
      121 accgaacgac cgcaggagcg ccgccgcggg cactgctggg tcgcccgcct cgcccagcac
      181 cgggtgcagc ccgtggtcgg cgaggtggtg gaacacgagg ttgaccactg tccacgggtt
      241 gaacgtctcg acgctctcgt tcacgttgat gctgtcgtcc acggtgcctc ttcccgatcc
      301 ggcggtctgc cgagcgtggg cgcggggccc tggcacggtc aagggccggg gtgtcgcaag
      361 ctgagacacc cggtcggttg cgccggtcgg ccacggctac gaccgtgccg gtcgtacgat
      421 gcctggcgtg tcctccgcac gggccgagcg tctggtgaac ctggtgctct gcctgctgtc
      481 cacccggcag ttcctgcccg ccgagcggat ccgccagatc gtgcccggct acgccgacgc
      541 gcgcggggac gacgcgttct tccggatgtt cgagcgcgac aagaacgagt tgcgcgagct
      601 gggtgtgccg ctggagaccg ggcgccggtc gagtttcgac accgtcgatg gttaccgcat
      661 cgcccgccag gactacgagc tcggcgacat cgaactggag ccggacgagg cggcggctgt
      721 cgcgctcgca gcccggctgt gggattcccc cgatctcgcg gtgcccgcgc acagcgcgct
      781 ggtcaagctg cgcgccgccg gggtcgaggt cgacgaggac cagggccgcg tgcagccgcg
      841 ggtgcggacc accgagccgg cgttcgcacc cctcctcgcc gccgtgcggg ccgcgagggc
      901 cgtcacgttc gagcaccgcc gcggcggccc gggtggcgtg gtgcagcgcc gcaccgtcga
      961 gccgtggggc gtcgtgtcct ggcgggggcg atggtacttg gcggggcacg accgggaccg
     1021 cgatgcgccg cgctcgttcc gggtgtcccg gatcagcgag gccgtcaccc ccatcggccc
     1081 ggccggcgcc gtcacggtgc cggccgacgt cgacctgatg gcgatggtgc gtcgcaacgt
     1141 cgatcccccc gcggtcaccg gcacggccag ggtgtgggtg gccgacagca gggcccacgg
     1201 cctgcgccgg ctcgctcggg cggtcgggcg gtacacgcac ggtggcaggc ccggcgacga
     1261 gctggagctc gacctgcgca acgtcgagac cgtggcccgg tggctcgccg gccacggtcc
     1321 cgacgtcgcg gtgctgggcc cggagagcct ggcggcggcg gtccgcgcca actgggcggc
     1381 cgcggcggcg gcccacgcca cccgcgacct gcacgccgac ggccgtttcc cggtgtccga
     1441 ccaccacgtc gtggtacgtc cgggccgcgc ggggatcggg ccgtgagcac cggcggggtc
     1501 accgagcggc tgccgcggct gctctctctg gtgccctacc tgctcgcgcg accggggatc
     1561 ccggtcgccg aggcggcggc tgacttcggg gtcagcgaac ggcagctgcg ccgtgatctc
     1621 gagctgctct ggatgtgcgg ccttcccggc tacgggccgg gtgatctggt ggacctgtcg
     1681 ttttccggtg acaccgtcac cgtcacccat gacgcaggca tgcgtcgtcc gctgcggctc
     1741 accacggccg aggcaacggc gttgctggtg gcgctgcgca cgctggccga cgttcccggc
     1801 gtggtcgaca cggcggcggt gcagcgggcc accgcgaaga tcgagcgggc cgtcggcgac
     1861 gccgggctcg tcggcgtggc cgtcgacctg tcccgcgagg aggcggccac caccgcggcc
     1921 gtgcgcgagg cactggccgt gggccgcgcg ctgcggatcg tctactacac ggccggtcgc
     1981 gacgcggtct cgcagcgcac gatcgacccg atgcggttgc tgatcgtgga ggggcgcggc
     2041 tacctggagg cgtggtgccg gcgggttgag gcggtgcgga tgttccggct ggaccgggtg
     2101 gaggaggtcg aggtgctcga cgagcccgcc gccccaccag cggaggccga acccaccgac
     2161 gtgtcagagg gcctgttcca gccgagcgcc gagcaccggt tcgcggtcct gctgctggag
     2221 cgcgaggccc ggtgggtggc ggagtactac cccgtcgacg acgtggtgga gctcgacggg
     2281 aaccgggcca gggtgctgtt gcgctacgcc gacccggcgt ggctcgtccg gctggtactg
     2341 gggctcggtg gccgggccca gctgctcgaa ccgccggagc tggtcgacgc cgtcggcagc
     2401 agggcgcggg aggcgctggc agcggccggc gggcacggcg atcgtgacac ggttgagcag
     2461 agtgggggat gaaccatggg tgagttcctg cccgcgctgg tgacggccgc ggtcggcctc
     2521 atactgctcg tgctcgtgct gctgctggtg ctcaggtccg tgcaccgggt ccgccgcgcc
     2581 agcgcggcgt tgcgcctcga cacgggcgag cgcgccacct cgttgcacgc gttggtgaac
     2641 tcgcggcgac gcagccacga atgagtgacg acgtcttcgc cgcggcggcc cgtgcaccgg
     2701 acggctgccc cgggcggcgt agagtgcccg acacgtgacc acggcgggaa ggacgagaat
     2761 catgccgagc ctcggcggct gggagttcgt gatcctcatc gggatcttgg tgctgttgtt
     2821 cggcgcgaag cggctcccgg acatggcccg ttccatcgga cagtccgccc gcgtcttcaa
     2881 aggcgagatg aagggcctga agggcgagga ggcaccagca gggggcgacg ccgccagggc
     2941 cgagaagccc gccgatgcgt ctgcgccgcc cgcggcgctt cccgcaacgc agcccgccca
     3001 ggtccaggag accaaggcgg agcccacccg cgccacgcag ccgggctcca gcagcacagg
     3061 gcagtagccc agcccggtgg ccagactttc cttgcggcgg ggcggtaggc ggccgaagaa
     3121 ccccgacggc accatgtcgc tcgtcgagca tctctatgag ctgcgcaacc gtctcacgat
     3181 cgcgctcatc gcgatcgccc tcaccacgat catcggctac atctggtacg gcaccggctt
     3241 cttcggcctg cccagcctgg gaaacctgct caaggagccc tactgcgcca tccccgccac
     3301 ctcacgtggc cagttcagct ccgatcccaa cgcctgcacg cttctcgcga cgggcccgtt
     3361 cgaccagttc aacctgcggc tcaaggtcgc gatgacggcc ggcatcgtgc tcgcatgtcc
     3421 cgtgtggctg taccagctct gggggttcat caccccgggt ctctacgcga aggagcggcg
     3481 ctacgcgctg agcttcgtct ccgtggccgc ggtgctgttc gtctttggag cggtgctggc
     3541 ctacctggtg gtctcgcagg gcctgagctt cctgctcagc atcggcgaca acgtccagac
     3601 cacggcgttg acgggcgagt actacttcag cttcatcatc gcgctgctgc tgatcttcgg
     3661 ggtcagcttc gagatccccc tgctggtggt gatgctcaac ctcgccggcg tcatctccta
     3721 cgcgaagctc aaggcgtggc gccgcggcct gatcttcggt ctcttcgtgt tcgccgcgat
     3781 cgcgaccccg gggcaggacc cgttctcgat gctcgcgctc gcgctcgctc tcgtgctgct
     3841 gttcgagatc gccatccaga tcacgagggt caacgatcgg cgccgcgcgg cccgacgcgc
     3901 cgcggagggc tgggacgagt gggaccccga cgcccccagc ccgatcgaca ccagccccag
     3961 ccagatcgac accacgccca gccaggtcga caccaccccg agccccatcg agagccccag
     4021 cccgatcgag accttgcctg cctccgatgc cgcccgtcgg gtcgacgacg ccacctgagc
     4081 ccgctaccgg tgcgcccgca tatcgggagc gagcacgtcg gtggcacggt ggtcgtcccc
     4141 cactccgtcg accagcaccg cctcggccag ggagttctcg gtgcgcagcg gcaggatggc
     4201 ccggtacgcg ggtgagtcgt accagccctg tgcctgctcc cggtcgggga aggcgatcac
     4261 gatgaggcgg ccggggaagt cgccctcgag caccgtgggc tcggccccgt gcacgatgaa
     4321 ccggccaccg aagggttcca gagtctcgtc gatccgctgc aggtactcga tgatcgccgg
     4381 cccgacggtg acctcgcgca tgtgggcgac ggcgtaggtg gtcatggtgt cctcccggtt
     4441 cgcggccgga gttctgcgac cgtcgacacc agcgtggacg ccggtcgggc gctggtcgat
     4501 gaccggcgag gtaagccgat gcttcgagct caggtctcct cgcgccacac catgatctgg
     4561 tcgccggcgc cctcgagcag caccgctgcc ggctgcaccc gcagcatcca cggctccagc
     4621 cgcagcgcag cgaagccggg ggagagcgga ccgtcccagg ccgggatgat ggccgggtcg
     4681 tagccgaccg gctccggccc ggtgcggaag cgctcccaca cctcggtccg cgtctcgtcg
     4741 tcggtgtgca ggctcgcgcg gcagtggacg ctggcggtgt cctgtgcggg gtcccagtaa
     4801 gtcagcgaga cgtacgggtg ggcggccagg tgcgcgcgct tggccggcgt cggaccggtg
     4861 gcaatccacc cggtcaggcg ctcgccgtcc cactgccaga tcgggtgtag gacgcgggtc
     4921 cacggcctgc cccgctcgtc cacagtggcg gccgtggccc acacgatccg atgcgccatc
     4981 tggacgaacg cgggtgcgat gtcggggagg gtggagggca tggggtgagc gtgccatgac
     5041 caggtgccgt gtcattgtgg atgggcaggg cgggttgatt tcacggcgat gtggaaacct
     5101 gggtcggtgg ccagcagcaa tcccgctccc gcggaggcat acgccgccgc caggagccgt
     5161 gccgcacgtc cccgtctcgc ggagttcgcg acggacctcc ccttcgagct cgacgggttc
     5221 cagcacaccg cgtgttcggc gctcgaggac gggcacgggg tcctcgtgtg cgcccccacc
     5281 ggcgcaggga agaccgttgt cggtgaattc gccgtgcatc tcgcgctcgc ccagggactc
     5341 aagtgcttct acacgacgcc gatcaaggcg ctgtcgaacc agaagtacgc cgatctcgtc
     5401 gagcgccacg gcgccgaggc ggtcgggttg ctgaccggcg acaccgtcgt caacggggac
     5461 gcgcaggtgg tggtgatgac caccgaggtg ctccgcaaca tgatctacgc cgggtcacga
     5521 cagctcgagc agctgggctt cgtggtgatg gacgaggtgc actacctcgc cgaccgtttc
     5581 cgcggcgcgg tctgggagga ggtcatcctc cagctcccgg agcacgtcgc gttggtgagc
     5641 ctgtcggcca ccgtgagcaa cgccgaggag ttcggcgact ggctggtgac cgtgcgcggc
     5701 gacaccaccg tggtggtcga cgagcaccgg ccggtgccgt tgtggcagca catgatggtc
     5761 ggtagccggc tcctcgacct gttcgcgggc gaagacgccg cgggcgagct gcaggtggat
     5821 ccggacctcg tgcgctacac ccgcgacctc gaccggcaga gcgccgtgtg ggaccggggg
     5881 cgtggacggg ggtcgcgcgg cggagggaac ggtcgttcct ccacgcgttt ccgccccccg
     5941 tcacgtacca cggtgatcga ccgtctggac cgcaacgggc tgctgcctgc gatcacattc
     6001 gtgttcagcc gggcgggctg cgacgccgcg gtcggccaat gcgtgcgctc gggcctgcgg
     6061 ctgaccaccg aggaggaagc gctcgagatc cggcgcaccg tcgagcggcg caccgccgac
     6121 ctcccgcagg gcgacctcgc cgtcctcggc tactgggagt ggctcgaggc gctggagcgc
     6181 ggcatcgcgg cgcaccacgc cgggatgctg cccgcgttca aggagaccgt ggaggagctc
     6241 ttcgtccgtg gtctcgtccg cgcggtcttt gccacggaga ccctcgcact cgggatcaac
     6301 atgcccgcgc gcaccgtcgt gctggagcgg ttggtcaagt tcaacggcga ggcgcacgcc
     6361 gagctgacac ctggcgagta cacgcagctc accggccgcg ccggccgccg tggcatcgac
     6421 gtcgaggggc acgctgtcgt ggtctggcag ccgggtgtcg acccggtgca ggtcgccggg
     6481 ctcgcgtcga ggcgtaccta cccgctgcgc agctcgttcc ggcccggcta caacatggcc
     6541 gtcaacctcg tgggacggct cggcacggag gccgcccgcg agctgctgga acagtcgttc
     6601 gggcagttcc aggccgaccg ctcggtggtg ggactggccc ggcgcatcga gcggaacacc
     6661 gaggccctgg ccggatacga cgagtctatg cactgccacc tcggcgactt cgccgagtac
     6721 gccgcgctgc gccgccaggt cggcgaacgg gagaaggcgc tgtcccgcca gggcgtggag
     6781 gacagccgcg aggccgcggc cgactcattg cgctcgctgc ggcccggcga tgtcatcgcg
     6841 gttcccggtg ggcgccgggc aggtctcgcg gtggtgctcg accccggcct cgacaaggac
     6901 gccgacccgc gtccgctcgt gctgagcgag gaccggtggg cgggcaggct ctcgcctgcc
     6961 gacttcccca cgcccgtcga gccgctcgga cggatgcggc tgccccggcg cgtcgaccat
     7021 cggacgccga gcgtgcgccg tgacctcgcg tcgtcgttgc gcaacaccgg gatcgtcgct
     7081 cccgccacgc cgcggcggcg ctcgcgcggc gggaatgtcg acgatccgga gctcgcgacg
     7141 atgcgccggg ccctgcgcgc ccacccctgc catggctgcg acgaccggga ggcgcacgcc
     7201 cggtgggccg accggtacgc gcgcctggag cgcgagacgg agcagctgcg ccagaaggta
     7261 cgggcgacca cccactccct cgcgagggcg ttcgaccgga tccggtcgtt gttgggtgag
     7321 cggggctacc tcgacgggga cgagatcacc gaacacggcg agcggctcgc ccgcctctgg
     7381 ggcgagtccg acctgttgac cgcggagtgc ctgcgccacg gcatctggga ggggctcgag
     7441 ccgccggagc tggcggcggt cgtctcggcg ctggtctacg agtcccgacg ggacaacggc
     7501 cagatgccac gggtgccgtc cggcgccgtg tcggaggcac tcacgagcac ggtgcggctc
     7561 tgggccgagc tggagggtga cgagcggagg caccgggtgg accgcacccg ggagcccgat
     7621 ctcgggttcg cctggccgat ccaccgctgg gcacgcgggg agtcgctgtc ggtcgtgctg
     7681 acggcagccg agcagaatgg cgccgagctg tcggcgggcg acttcgtgcg ctggtgccgt
     7741 caggtgctcg acctgctcga ccagatcgcc ggggtcatgg gccgctccag cggcgtgggc
     7801 cgcgccgcgg cgcaggccgg cacggcgatc cgacgcggcg tcgtggccgt tggtaccgtc
     7861 tgagcggtct cgctgtgcgt gggcggttgg ctgtgctttg atcgccgcac cgcacggtgg
     7921 cagaaaggtc gacgatgagc acgccccagg gccccaaccc cccgaacggc acaccgcacg
     7981 ggcagccggc ggagcaaccg gcgtggggcc agcagccgcc cggcggctgg gggcagcagg
     8041 gcgagcaggg atgggatccc gacgagacgc gcggcgtccc gacgcatggc ggacccgggg
     8101 ccgcccagcc cggctacgcc gaccagaacc cgaacggaaa ctctcagcag cagtacggtt
     8161 cgcaaacaca ggaccttccg cagtacggtc cgccgcccca gcagggccta ccccagtacg
     8221 gccacccaca gcagggcccc ccgccgtatg gccaggcgcc tcagcagggg caaccccaga
     8281 acccctcgca gtacgaccag ccgacccagc agggtcaacc cgcctacggc cagcagccgt
     8341 acggatggcc ccaacagagc caaccgcagt atggccagac ggggccgggc caaccgcggg
     8401 gcgcggagtg gccagggcag cagcagggct ggggccagta cgcgcccgcc cagcagtggc
     8461 cgggtcagcc gcagggcacg cagcagtggg gacagggccc ggagccggcg aagcccagcc
     8521 ggagcgggaa gaagaaattt tcatcgtcgc tcatcatcgg cggcgccgtg gtcgtcgttg
     8581 cggtgatcgc agtgctcggg ttcgtcgcgc ccggcttctt cgtcaccagg gtgttcgaca
     8641 cggccgctct gcaggacggg gtgcagcgcg tcctcaccca ggactacggc ctggcgaacg
     8701 tcgcaggcgt gcgctgcagc gaggggatcc gcgtggagca gggcgccaca ttccaatgcg
     8761 acgccacggt ggacgccgct ccggtgaagg tcccggtcac gatcaccggc tccgacggtg
     8821 cctacgaggt gggccgcccg ggctgaggtc gcttccacgc aaaggggttg ccgcccgcgc
     8881 cgggtgatcg ggcagcatcc gccctgtgac ccatgacgtc cgcaccctcg atcccgacca
     8941 gctgcgctcg gcgcacatcc tcttcggctc cacgatccac cggggtccgg tcgacgatga
     9001 atggtggtcg cgcgcagcca ccagctacag ccccgggcgc accgtcggga tccacgacgg
     9061 gggcgcgctg gtgggcacgg cgatggcgtt cccgagccgg ctggtcgtgc ccgggggcgg
     9121 cgtgctgccg atggccgcgg tcacgcgggt gggggtgcgg gccgactgca cgcggcgcgg
     9181 cctgttgtcg gccatgatgc gggcccagct cgacgacgtc gcggcccgtg gtgaaccggt
     9241 cgcgacgctg cgcgccagcg aaaccaggat ctacgggcgg ttcggctacg gggtcgccac
     9301 ccgcgggcgc gacctgcggg tgcgggtcgg ccgcccggca tggcggccgg atgcagcgca
     9361 gggcgggtcg gtgcggctgc tcgaccgcga cgacatcatc ccgacactgc gggaggtgca
     9421 cgagggcatc gcactgaccc gggccgggag catgaccagg caggacagct ggtggtgggg
     9481 tgcgatcggg cgaagggtcg aggaccgcga acccgtcatc gcagccgtgc acacgggggc
     9541 gcacggggac gacggattcg ccgtcgcatt tcgcaccgac gccgacagct tctccgagca
     9601 gacgctcgag gtcgcggacc tgcacgccgc ggacatggag gcgacggcgg gactctggcg
     9661 gttcctgctc gacatcgacc tggtctcgtc ggtgcaggcc gagctacggc cgctcgacga
     9721 gcccctcgag ctcctgctgt ccgacccgcg ggcgtgcgcc gtcaccgggc accacgacga
     9781 gacctggctt cgtctcgtgg acgtgcccac agcactggcc gcccgctcat acggcgccgc
     9841 ggaacccgtg ctgctcgccg tgcatgaccc actgctggag aagaacgccg gcgtctaccg
     9901 gatcgccggc ggcggcgcgg agcgcgtcgc gccgctcggc agtccgatgc aaccgcagct
     9961 ggagtgcgac gtggcggcgt tggcgatggc ctacctcggg gaccgcagcc cgtcgcagct
    10021 cgtggccacc ggctggtggc gcgcgcacga cggcgcagcg gtgcaccgcg cggacgccgc
    10081 cttcacgacg tccgtcacgc catggtgcgg cacccacttc tgagacccgc aggtggctac
    10141 cgggcgagcg cggtgaggag ccggtcgatc gagctgccga cgccccagcg ctcggccagc
    10201 gcggccaggc ggtcggggtc ggccggggag ctgggcttct tgtcgggccg gtcgagctgc
    10261 accggggcgt ccagcgccac ccgcaccacg ggttcgacga cggcgaggta gggcgccgcg
    10321 gcagccagct tggtgcgtac gggggggctc atccgcacgt cgctccggtc ggtgacggcg
    10381 gcccgaagct cggcccacga accgaagcgc ccgacgagcg tggctgcggt cttcgcgccg
    10441 acgccaggta ccccgggcag cccgtcggac gggtcgccgc gcagcatcgc catctcggcg
    10501 taggcctcgc cggcccgtgc ggcgggcacg ccgtacttct cggccacctc gaccgggccg
    10561 aggtgctcgg ccttcgccag ccctctgccg acgtacagca gccgtaccgg gaccggctcg
    10621 tcgcggacga tctgcatgat gtcgcggtcg ccgctcacgg ccagcaccgg gtcctcccgt
    10681 tcggccgccg cgagcgtccc gatcacatcg tcggcctcat agccctcggc gcccgcggtg
    10741 gcgatgccgg ccgcggccag cacctcgagc aacaccggga cctggggcgc gagggtgtcg
    10801 ggcacctcct cgggcagccc ggcgggcgcg acctcgggct caccgggcac ccggtgtgcc
    10861 ttgtaggacg gcaacgcctc gacgcggaac gcagggcgcc agtccaggtc caggcacgcg
    10921 acgagccggg tgggccgccg atcggtgatc agccgcgcca ccatgtcggt gaacccgcgc
    10981 accgcgttga ccggggtgcc gtccggggcc gtcatcgact ccggcacccc gtagtaggcc
    11041 cggaagtaga ggctcgcggc gtccagcagc atcaacgagg gtgcaggcac ggtcacggtg
    11101 gcacagcttg ccggaatacc ctgctctcca tgacaccggc cgttcccagc gcgctgttcg
    11161 ccgaccgcct ccgccgcgcc ggagaggtgg cggcggttca cgacaccgac ctgctgctcg
    11221 tcacgcctgg taccgatctg cgctacctga tcggcgccga aggctcgtcc cacgagcgtc
    11281 tgacctgcct cgtcctgccc gccgccgggc accgggcccc gcctgcgctc gtcgtgccac
    11341 ggctggaggc gccgggcctc gccgggctgc cgctcgagga gctgggtgtg gagatcgtca
    11401 cctggaccga tggtgacgac ccctatctcc tggtgagcga tctggcaggc gggccaaccc
    11461 ggatcaggct ggtggactcc atgccggccg cccatgtctt cggtctgcgg gacgcgttcc
    11521 ctgaggtgac acaggagctg gccggcccgg tgatccgcga gctgcggatg cggaaggaca
    11581 cagccgaggt ggccgaactg cgggcggccg gtgccgcgat cgaccgcgtg cacgcccgga
    11641 tgggtgagtt cctgaagccc ggccgcaccg agaagcaggt gggcgccgac atcgccgcgg
    11701 cgatcatcga ggagggccac acgcaggcgg ccttcgtgat cgtcggctcc gggccgaacg
    11761 gggcgagccc ccaccacggc gtgtccgatc gtgtgatcga gagcggggac gtcgtggtga
    11821 tcgacatcgg gggcccgctg ccgagtgggt acaactccga ctgcacgcgc acctacgccg
    11881 tgggagagga gccttcggct tcggtgcgcg aggcttacgc agtgctgcag gacgcccagg
    11941 agcgagccgt ggccgccgcc cggccgagtg tcaccgccga gcaggtggac gccgctgccc
    12001 gcgagccgat catggcggcc ggactggggg agcggttcgt ccaccgcacc gggcacggca
    12061 tcggcctgga cgtgcacgag gagccctaca tcgtgggggg caacgagctg cagctcgagc
    12121 cgggtatggc gttcagcgtg gagcccggcg tgtacctgga cggcgagtgg ggcgcccgga
    12181 tcgaggacat cgtcgtggtg accgacgacg gctgcgagcg gctcaacacc cggccccgcg
    12241 acctcgcggt gctctgaccg cgccttctgc gcgctgaccc cgtcctcgca gggcccgtcc
    12301 gtgggccctg cgagggccgg ccggaacacg cccgaaaggg gcgatgacag ccggttctgt
    12361 accgaatcgg ttccaggctc aggcagaggc ggccaggctc gggaagctca cggccggacc
    12421 ggcggtgtcg agtcagcagg cgtgtagatc gtcagtcgta gcgcggggtc gtcattggct
    12481 tggaaggtcg cgtactcgaa gcagagcgtg cccttggcgg gatggtgcag gatcttctgg
    12541 ccggcgccgc tacccagaac gtcgtgctgt ccccaccacc tgtggaattc gggacagccg
    12601 tggcggagtc gggccgacag ctcctcgaat gccggatctg gtgcccagag atcgtgcgtt
    12661 gcccggaatt gggctacgac gtgtgcggcc tggtcggccc agccgtcgcc gaagaggcgt
    12721 cgggcatcgg ggtcgagcag gatgtagacg aggacgttgc ggtcctcgtc ggtgacacga
    12781 ccgaagtcgg tgaacagatc gaccgcggcg gcgttccagg ccagcaggtc ccatcgacgg
    12841 ccggtgatgt aggcaggttg actcaggctc tcgacgaggt gcctgatcgc gccaggcacg
    12901 cgctcgcgga cgaaggcggc cttagccggg ttgcccgcca gtgcccgcag atgaatccgc
    12961 tcggtgtcgt cgaggcgaag cgcgcgggcc aaggcgtcga tcgtggtggt cgaggggctg
    13021 acagttcgcc cttgttcgag gcggacgtac cagtcgactc cgatgccggc gagttcggcg
    13081 acttcttcgc gtctgaggcc gggtgtgcgg cgcttgcgcc gcgtcggcag accgaccacc
    13141 tcaggagtga gtctttcccg ccgtgatcgc aggaagtcac ccagccctct gcggaccggc
    13201 tcgggcacaa ccgtcgcctc tcgtctgggt gggactgact aatcctacga cgacgctagg
    13261 cctggatacg gcgccgccgg ttgcccacga tcggtccggc accgagacgg ccggcacgag
    13321 gagcaggaac aactatgaaa gcggcagttc tgagcgcatt cggctcgcca ctctccgtgg
    13381 agagcgtgcc ggacccgacc ttgggaacgg gcgaggtgat cgtcgacgtc gtggcggcag
    13441 gcgtgctgcc ttatgcggct gacgtcctga gcggcaagcg gcggcacctc ctcaccctgc
    13501 cggtggcacc cggagccggg gcggtgggcc gcgtacgcgc cgtcgggccg gacgcgaccc
    13561 gactggccgc aggcgactgg gttctgtgcg acccgaccgt gcgctcgcgc gacgacccgc
    13621 tgactccgga catcaccctg caaggcttga gcgcccgcgg cgacggcggc ctgcgcctgc
    13681 agaggcattt cgggcagggc tccttcgcag agcaactgct ggtccccaca gagaacgcct
    13741 tcccgctcgg ctcggtcgac ctggcccatg cgggacggtg gactgcgctg aacctgtgtc
    13801 tcgtgccgta cggcggtctg ctcgcggccg acctgaaagc gggtgagacg gtcctcgtca
    13861 gcggcgcgac cgggaacttc ggcagcgccg gcgtcgccgt cgccctcgcg atgggagccg
    13921 gctgcgtcgt cgcgccgggc cgcaaccagg acatgctcac tgagctcgaa cgccgattcg
    13981 gttcgcgcct ccgaaccgtg tcgctgtccg gcgacgagga ccaagatcgt gagcggatga
    14041 cacaggcggc gcccggcccg atcgacgtcg tcctcgacct gctaccgccc tccgcgggga
    14101 cgactcccgt gcgggccgct gcgatgactg tccgggagta cggcagggtt gtgttgatgg
    14161 gcggcgtcgg catgctcggc ggcgatgacc tcgccctgcc ctatccgtgg atcatgcgga
    14221 acagcattac cgtccgcggg cagtggatgt acccgccgca ggcgaacacc cgcctcatcg
    14281 agctggcccg atccggtctc ctcgatctcg gtcaattcga cgtcaccgag ttcgcactcg
    14341 acgatgccgc caaggccatc gcacacgccg ccgacagcaa cggacgattc acactgaccg
    14401 tcatccgccc atgaccctgt tcacagacgc tcaggcagcc ggggcgagca cggtgccttc
    14461 caggtgcgtc ctgccgtcgg cgcccgcgac gcacgcagct gcgggggcgc ccgaccgcac
    14521 gaaatccccg acgcaccggc ccatctcggc gtgcgccccg gcagtgggat ggaacgactc
    14581 ctgggcgagg tggtagccgg ccgcgtcgag gccgccgtgg acgaacgcct gcgggtcgac
    14641 ggtgatccgc cgctgccact cctggctgtt cagagcgcgg ctgcaggcct cgaagccctc
    14701 ggtggcgcgc gtcatgtcga gaaaacgcgt gccggtgcgc tgggcgacgc cgccgagcgc
    14761 ggccgacaga gcggggaaca gtgcggtgcg gccccagccg gcgtcggcct tgctgtaggg
    14821 gcagccctgc aaaccctgca ccggcaccat ccgctcggtg acgggcgagg cgtaggacgc
    14881 cagcacgagc tcgtagctcc cgtcggcgta gccggcccgt cgcatggcct cctgcacgtc
    14941 tcgcaccgcc ctctccacct tcgcagcagt ggcccccatc cggtcggcca cgagcgggcc
    15001 gagggtgccg cggcacggtg gttcgcgcgg attgaggaac gcccggatgc aggcgatgcc
    15061 ggtgccggtg agtgcggcgt cgtcattggc gccgacctgc aggacgacgg tggtgacctg
    15121 gtagcgggcg gcgacgtcga cgagccgctg cgcctgggac ccctcggtgt tgtgggtgcc
    15181 ggggccgaat gcgacgtccg ccgcagcggc gccggagcac gccaggttga ccgcctcggc
    15241 ggcgagcccg gtgcggcgca cgtaagcgtg ggccgagcgg tggcaccagt cgccaccctc
    15301 tccccgggta ccggactcgt agtcgcccgc gccctcgccc gacgcggcgc tgtcgcccat
    15361 ggcgaccacc gcggtgggcc gcgctgcggc cgcggccgcc ggagcgccca gcagccccag
    15421 cggtgccgcg gctgctgcaa cggcgagcat ggcgaggagg gagcgggatc gcatcacgag
    15481 gatgacgcta gggctcgtcc tcgagaactg acagggcgtt caccgagcac tgtggacagc
    15541 gggcgatcct tgcccgcgcg gagcagcgcg gtctcaccgt ccgcgagtgc cgtcagccat
    15601 ccggggtacc tcgggggtgg aatctcaggg gcgactccgg ggcattcacc cagcacaggc
    15661 cccggccaca gtggagactg gacctcagcg gcgagagtcg ccgcgacctg gaaggagccc
    15721 ctcgtggcat cgaccctgac ccgcccgcga cacgggaagg tcatcgccgg cgtctgcgcc
    15781 ggactcgctg agcgcttcgg ctggagcccg ttcgtggtcc ggctcgcgtt cctgctgtcg
    15841 tgcctgctgc ccggcccgca gttcgtgatc tacctcgtgc tgtgggtgat catgccgaag
    15901 cggccctact gatcattgag tgcggtagac aggcggggtg actcgcatcc tgctgctcgg
    15961 cgcccgggtc ctcgacccgg gcggcccggc aggaccggat gccgtcgaga tcagcgacgg
    16021 caggatcacc tttgtcgggc gggtggcgca cgcgcgggag cggatgcggg gcgcggccgt
    16081 gctcgagttg gacggggcgg tgctcacccc ggcgttcgtc gacgcccacg tgcacgccac
    16141 cgccaccggc ctgctggtcg acggcctcga cctcaccggc tgcgcgtccc ccgaggcgtt
    16201 gctcgacgcc gtcgccgccc gcgccgccgc ccgaccgggc gcgctggtct ggggccacgg
    16261 ctggcaggac cagcactggc cgcaaccgcc gcccccgcgc cctgcgctcg accgcgccgc
    16321 cgccggcgcc cccgtctacc tgagccggat cgacgtgcac tccgcgctcg tgtcctctgc
    16381 gttgctcgac cgtgccccgg ccgccgtcgg cgccccgggc tggtccgacg gcccgctgtc
    16441 tgccgacgca caccacctcg cccggcgggc ggcgctgacg gccgtggacg gcgtcgcccg
    16501 ggccgccgcc cagagcgcct ttctcaccgc cgccgcggcc cgcggtgtcg cggtggtcca
    16561 cgaatgcgcc ggtcccgaca tctcctcggc ggccgacctg gccgagctgc tcgcccggcc
    16621 cggccccgag gtggtcggct actggggcga ggcggtgacc acgcccgggc aggtccggga
    16681 gctgctggcc acaaccggtg cctgtggcct ggcgggcgac ctcttcgtcg acggctcgat
    16741 cggatcccgc tccgctgcgc tgcgagcgcc ctacgccgac gccgaaggct gcaccggcaa
    16801 ccggtacctc gatgccgacg ccgtggccgc gcacgtcgcg gcgtgcaccg ctgccggcgt
    16861 gcaggccggc ttccacgtga tcggcgacgc cgccgcggac gtgctgctgg cgggtctcga
    16921 caccgcggcc acggccacct cggccgcagc ggtgcaggcc gccgcccacc gtgtcgagca
    16981 cctcgagatg ctcgaccctg cccaggcccg ccggctggcg cagctgggcg tcgtcgccag
    17041 cgtgcaaccc gcgttcgacg ccctctgggg tgggcgcgcc gggatgtatg ccgagcggct
    17101 cggtgccggc cgggccgcga ggatgaaccc gtttgcggcg ctccgggacg ccggtgtcgt
    17161 gctcgccctc ggctcggaca cgccggtcac tccgctggac ccgtgggccg ccgtgcacgc
    17221 cgcggtccac caccagacgc ccggttcggg gttgacgccg aacgaggcca tggaagcgca
    17281 caccgtcggc gggtaccggg cggccggtga ccgttctccc ctcgcggggc ggctcgtgcc
    17341 cggtgcgccg gcgtcctacg tcgtgtggga cggggcaggc ccgctggccg gcgacccgca
    17401 cccgccgcgc tgtctccgca ccgtgcaccg gggggtgatc ctgcacgacg ctctgcggga
    17461 tgtgggaaca ttcccgccat gacggaccgg acgggctttg cgggcgtggc ccgctgctgt
    17521 ccggggtgct gtcgctgtcg ctgtcgcagc tgacttcccc cgacaacgtc catccgccgc
    17581 cactgcggcc cgcacagtct gcgatcacga ccgtgacccg ttcccccgac ctgcgtggtc
    17641 agaccgttct cgtgctgcac gcccatcccg acgacgaggc gatcttcacc gggatcaccc
    17701 tgagccgcct ggccgaggcg ggtgcgcgca ccgtgctcgt cgtagccacc gccggtgagc
    17761 tcggacagtc gcgcctgccg ctgcgaccgg gcgagacgat ccccgagcgg cgggtggccg
    17821 agctcgagcg ggccgccggg ctgctcgggg tctcgcggct cgtcctgctc ggcaggcgag
    17881 actcgggcct ccccggctgg tcggacgggc agcacgccag ggcgctcatg gccgccgacc
    17941 cactgcggct ggcacgccac gtcgccgaga tcgctgacga cgagggagct ggcaccctcg
    18001 tccacgacga cgagcacggc atctacggcc atcccgacca ccgggccgcg ttccggatcg
    18061 gggccacggc cgccgagctg gtgggagcga cgggctaccg ggtcacggtc gaccgcgagc
    18121 acctgcacat cacggcgcgg gaccagcacc tggtgcacgg cgcggcccgg gccgcggcag
    18181 tgcccttcgg ccgggtcacc gccgagatcg gcctggccgt gtccgggtct cccggacatc
    18241 tggggcgcaa gcgggcggcg atgatctcgc attcctccca gatcgccccc gaggacgtgc
    18301 cggacgccgg cttcgcgagc gcgtacggca tcgagtggta ccggcgcagc ggtgagcccg
    18361 gccttttgga cgaactgggc aacgtgcacc tgctcgccgg agcgccggcc tgaaggacta
    18421 cgcggttacc aagcggcgcg ctgcggcccg tccgaacgcg gatcaccgtc ctcacacgac
    18481 cgcgccgtag gctccctccg tggccgcacc caccgccagc cccgtcgcgt ccgatcccgg
    18541 gccgcccccc gccgcccgtc ggcgccgccc gtggtgggcc gccttcgccg cccggctggt
    18601 cggctcagcg ggtggcgggt tgctgctgta cctcagcttc cccccgcgcg cccagtggtg
    18661 gctggcgctg ccggcgttcg ggctcc
//
LOCUS       DASRSC010000314        10386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_949353,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000314 DASRSC010000000
VERSION     DASRSC010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10386
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949353"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VFE39_15455"
     CDS             <1..440
                     /locus_tag="VFE39_15455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6260033.1"
                     /translation="LNLTAPFRMLRRAVPGMVEQGFGRVVAIASVAAKRGEPYISAYT
                     ASKHGLLGLVRSAASELSTTGVTVNAVCPAYVDTPMTDSTVASISSITGRTPEQAREA
                     LERRQPIGRLVTPDEVADAVLLCVTSGAITGQGINVDGGAVQS"
     gene            459..904
                     /locus_tag="VFE39_15460"
                     /pseudo
     CDS             459..904
                     /locus_tag="VFE39_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230092.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
     gene            complement(960..1805)
                     /locus_tag="VFE39_15465"
     CDS             complement(960..1805)
                     /locus_tag="VFE39_15465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020641546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaX family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HET6260034.1"
                     /translation="MTTVEEAPVVAGVVQPRQLIVTLYGLYARDDGGRLSVAVLVRLL
                     AGLGVDEPAVRSSISRLKRRGLLAPDRRDGSAGYVLTAVAREILDDGDVRIFGRRRAT
                     EADGWVLVVFSVPESEREKRHALRSQLSRLGFGIAAPGVWIAPGHLAGEVTDVLRRRG
                     LDGYVQLFRGEHLAAGGLPDAVRGWWDLEGLRDLYAAFLDRHRPVRARWSRRRTPDQA
                     AAFADYVRLVTDWRRLPYADPGLPLHLLPPRWNGVRAAKLFAELADRLTEPARAHARE
                     LIAGR"
     gene            complement(2095..2469)
                     /locus_tag="VFE39_15470"
     CDS             complement(2095..2469)
                     /locus_tag="VFE39_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260035.1"
                     /translation="MSYSWGAGRNARVEGRHGRAALEAVPAAPAEQIPAPPVADGRAA
                     APGAEHQRVAAELAPMVEVWQRLLAEHVPDRGGRCRTCTKGGTGLPSTPWPCSIHGIA
                     AMARRHHDADLAAATRRQRLSS"
     gene            2559..2795
                     /locus_tag="VFE39_15475"
     CDS             2559..2795
                     /locus_tag="VFE39_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF397 domain-containing protein"
                     /protein_id="HET6260036.1"
                     /translation="MILHNHLHVHVGTNSARWRKSTASNPSGNCVELAELAEGMIGVR
                     NSRHATGPVLVYTRAEIAAFFVAVRHGEFDDLAG"
     gene            2839..2970
                     /locus_tag="VFE39_15480"
     CDS             2839..2970
                     /locus_tag="VFE39_15480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260037.1"
                     /translation="MNLLTTLRKRERQYSWGERGAAMNGAATVSGTTTSGTTIVEQP"
     gene            2967..3902
                     /locus_tag="VFE39_15485"
     CDS             2967..3902
                     /locus_tag="VFE39_15485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6260038.1"
                     /translation="MSAVPDHVEPDGRVPGGGPEDGQPGSIIRGAASGPTVLRMGLGG
                     ELRRRREAAGVTRERAGEAIRASPAKISRLELGRVGFKERDVADLLTLYGVGEQDERA
                     EFLALARRANAPGWWHRYADLLPSWFETYIGLEQAASLIRTYELQFVPGLLQTHEYAR
                     AVTRLGHTDPTEVERRVELRMRRQEILTAANAPTLWAVIDEAALRRTLGGPELSRAQL
                     THLIEMNELPNVSLQIAPLRSGGHAAAGGPFAILRFAEPDLPDIVYLEQLTSALYLDK
                     RTDVDHYAAVMDRLGAQVDPPDRTADIIAQVRAEL"
     gene            3933..4892
                     /locus_tag="VFE39_15490"
     CDS             3933..4892
                     /locus_tag="VFE39_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HET6260039.1"
                     /translation="MRRKLRSPLPQRHGLDAVRLRTPAGTSWTTMRDHLVERLPRVDP
                     ARIDAMLREQRIVDRDGPIAPDAPYVPESFLWFHRDLPTEVPVPFEIGIVHRDDELVV
                     VDKPHFLATIPRGQHIVETALVRLRRELGLPTLSPAHRLDRVTAGLLMFVVRPERRGA
                     YQTLFRDRLVRKTYEAVARYDPQLALPRTARSRIVKERGVICAREEPGPVNAVTTVEL
                     LEHRSGPDGLRLGRYRLTPETGRTHQLRLHMSALGVPILGDDFYPVLTEKPLDDFRRP
                     LQLLASSLEFTDPVSGEERSFRTRIALQSFTSYAAWAGSWSPS"
     gene            5098..5466
                     /locus_tag="VFE39_15495"
     CDS             5098..5466
                     /locus_tag="VFE39_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260040.1"
                     /translation="MTARPAGRAAPTLEAVAARGRVPRNAGRVLAGSTTVGEQAREAV
                     LQAAADLSYVTNRAARSLMTRRSDSIAFVVAEPEERFFGDPHFVLVLRGAHGLRQALA
                     SRLLAVIERGRATRADGDEH"
     gene            5571..7010
                     /locus_tag="VFE39_15500"
     CDS             5571..7010
                     /locus_tag="VFE39_15500"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HET6260041.1"
                     /translation="MTTTQAAPSTDALRFPPGFLWGSATASYQIEGAVDEGGRTPSIW
                     DTFSHTPGRVERGNTGDVADDHYHRFREDVAIMARLGLASYRFSVSWPRITPQVTADS
                     LGPVNPEGIRFYSDLVDALLAVGISPSITLYHWDLPQSLEDTGGWTARSTAERFGEYA
                     EVVAAALGDRVPLFITLNEPWCSAYLGYASGVHAPGRTDDAAALTAVHHLNLAHGLAA
                     GSIRRAAPASRVALTLNLAWVCPETDSPQDAEAARRVDGLQNRVFLDPVLRGSYPADV
                     QADTAAVTDWSFVRPGDLEVISAPLDVLGVNYYSPTRVRHWTRERPKEHADGHGEGKA
                     SPWIACDDVEFPFQHDRKTDMGWSIDPRGMTELLRRVAREAPGCEIMVTENGAAFPDV
                     VNGDGRVVDTDRIDYLRSHLAAVHEAIEDGAPVTGYYLWSLLDNFEWAWGYAKRFGIV
                     HVDYDTQARTPKDSALFYADVIRRNAVPS"
     gene            7109..8740
                     /locus_tag="VFE39_15505"
     CDS             7109..8740
                     /locus_tag="VFE39_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260042.1"
                     /translation="MRLGDEDDLRKGPDSEDRDPKDSDNHDRDAQDRDSEHLPIDVAA
                     LRADDALVEELAAGLVALGELHHGARGSDDELVAMLAAWVADVRPDAQPTITASARDT
                     SPDEKALGAPSLVSLVRPASAHGRHRAAQSASEHGAIPYARRLAVAAAVIMMGTAGLA
                     VGSSDASPGEALWSVSKVFYAERARSVEAAAEVNTGFETARTALQQGRPADAARAIAK
                     ASALLDEVRPAEGRAELAHEHQLLLAALSGEPSGQSAAQAPVAPAPRPPDQPGTSSTP
                     ASTPTPSGSPVLASPPLPAESSTAPRAVAPPTGSGASAVPSVSGVPDNDTAGPSRSGS
                     ASTTVSVEDGTSPSTAPATPLPGSAPQPPTGPTSPTQPESQPQVDPSPQSPVQAGPEP
                     QPEPQPQPQSAPEPQTGSGPLSQPQSAPAAPGDTEGPGQPTGSGSANEKNGGSGGAGV
                     ANSATDGPEGNPGQGEPSQPSDSGGNSDNSTNSNNSGNSDNSGGRNNSESDGGGNSAD
                     GGNSDGGRNSDNGRGSLLSPATDLISSSLRSLRQS"
     gene            complement(8829..9149)
                     /locus_tag="VFE39_15510"
     CDS             complement(8829..9149)
                     /locus_tag="VFE39_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260043.1"
                     /translation="MDTPPFSFLGRPLGPSFRTTVVTLAPGTTRLYDESEWWDALVLV
                     ESGTIDLECRAGGRRRFCSGAVLCFTGLGLRALHNAGAASVVLVAVSRRRPAAGSGAD
                     AATT"
     gene            complement(9248..>10386)
                     /locus_tag="VFE39_15515"
     CDS             complement(9248..>10386)
                     /locus_tag="VFE39_15515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016336643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HET6260044.1"
                     /translation="AIWLVLHVGPSTRANALEPVEFTRFSAATQVQFRLDTELTITEP
                     TRVSIGGGPQQVLPPGRYQAQAGTSWVFDAGAAVPQVGGGRPPGGREWERPLLDPGGY
                     PVLATWSVLIATVFGTMGLPHILVRFHTSADGHGARRTAAITVGLLGVFYLFPPIYGL
                     LGAVLLPELYLSGGTDTVVVALPARVDQSWVGSLFTALLTAGAFAAFLATSLGLLLAV
                     SGAISHDLVPGTLRRLRFTPLAAAAVAVLLALPAARVDVGVLVTSAFAVAASTFCPLL
                     VLGIWWPRLTTQGAVVGMVVGLISSAGVIGVDLFLGAPPGLVAIVLGQPALWSVPLAF
                     ATMVLVSLRGRPPAWAAAAMLRLHLDENEAGSAAADDFRPPRMS"
BASE COUNT         1589 a         3879 c         3598 g         1320 t
ORIGIN      
        1 aactcaacct caccgccccg ttccggatgc tgcgccgggc cgtgccggga atggtggaac
       61 agggcttcgg ccgggtggtc gccatcgcgt cggtcgccgc caagcgcggc gagccgtaca
      121 tctcggcata caccgcgagc aagcacgggc tgctcgggct cgtgcgctcg gcggcgagtg
      181 agctctcgac caccggcgtc accgtcaacg ccgtctgccc cgcctacgtc gacacgccga
      241 tgaccgactc caccgtcgcc tccatcagct ccatcaccgg ccgcaccccg gagcaggccc
      301 gcgaggccct ggagcgccgg cagccgatcg gcaggctcgt cacccccgac gaggtggccg
      361 acgccgtgct gctgtgcgtc acgagcgggg cgatcaccgg ccagggcatc aacgtcgacg
      421 gcggggcggt ccagtcgtga ggtcggtgcg gtcgtgagat cagtgcggtc gtagatcggt
      481 gcggtcgtga tcgagcgcat caaccaccgg agctggcccg gccctccggg ttcgcccatg
      541 ccgtggtggt gaccggacgc accgtgttcc cggccggcca gaccgccctg gacgccgacg
      601 ggcggatcgt ccccggcggt ctggcggtgc agttcgagcg ggcgttgcag aacctgctgg
      661 tggcactgcg ggccgctggc ggcgggccgg agcacctggc gcagctgacg atctacatcg
      721 tcgacatgga cgattatcgt gcccgcgccc gcgagatcgg cgcggtctgg cagcgacact
      781 ggtcgggagg cagtatccgg cgacggctgg catcggtgta gcgcggctgt gggacgtcga
      841 ggcgctcgtg gagctgcagg gcagcgcggt gctgcccgcc gacgatcggg ccggcgaggg
      901 atgagcagcc ctcagcgcag gcactgctcg tcaccgaggc ccctgcccgc gcctgggggt
      961 caccgcccgg cgatcagctc ccgtgcgtgc gcccgtgcgg gctcggtcag ccgatcagcg
     1021 agctctgcga acaacttcgc ggcgcgcacc ccgttccagc gcgggggcag caggtggagc
     1081 ggcaggccgg ggtcggcgta gggaagccgc cgccagtccg tcaccagccg cacgtagtcg
     1141 gcgaacgccg ccgcctggtc cggcgtccgc cgccgtgacc atcgcgcccg taccgggcgg
     1201 tgccggtcga ggaacgcggc gtagagatcg cgcagcccct cgaggtccca ccacccgcgc
     1261 acggcgtcgg ggaggccgcc tgccgcgagg tgctcgccgc ggaacagctg gacgtaaccg
     1321 tcgagaccgc gcctgcgcag cacgtcggtc acctcgcccg ccagatgtcc cggcgcgatc
     1381 cagacgcccg gagccgcgat accgaacccg agccgggaca gctgcgaccg cagcgcatgc
     1441 cgcttctccc gctccgactc cggcaccgag aacaccacga gcacccagcc gtcggcctcg
     1501 gtggcgcgcc gccgcccgaa gatccgcacg tcgccgtcgt cgaggatctc ccgtgccacc
     1561 gcggtgagca cgtacccagc gctgccgtcc cggcgatcgg gggcaagcaa gccacggcgt
     1621 ttgagccgcg agatcgacga tcgaacggcc ggctcgtcga cgcccagccc ggccagcagg
     1681 cgcaccagca ccgccaccga gaggcgtccg ccgtcgtcgc gggcgtagag gccgtacagc
     1741 gtgacgatca gctgccgcgg ctgcacgact cccgcgacca cgggcgcttc ctcgacggtg
     1801 gtcacgcgaa cacgatatcg gctcggccca cacctcgcgc ttgcttgact atcgtcaccg
     1861 ccgcgccata ctcgatccgg gcaacactcg ctcggggagg tgccgcccca tggccagcgc
     1921 ccgcagcgcc catatcgatc cgttctgccg cgagcacctg ccgccctgtg agcagtggcc
     1981 ggagctgtgg tcgaccgtcc tgagctgcag taccccgaac acctcgtccg caggaggacg
     2041 ccgggcaccc ggcacctccg ctggcttccc ggcggggcgt agcaggtggt ccgactacga
     2101 cgacagccgc tgccgccggg tggcggcggc caggtcggcg tcatgatgcc gccgggccat
     2161 cgccgcgatc ccatggatcg aacagggcca tggcgtagat ggcaggccgg tgccgccctt
     2221 cgtgcacgta cgacaacgcc ccccacgatc cggcacgtgc tccgcgagca ggcgctgcca
     2281 gacctccacc atcggcgcca gctcggccgc gacccgctgg tgctccgctc ccggcgcggc
     2341 cgcacggccg tcggccacgg gcggcgcggg gatctgctcg gccggtgccg ccggcaccgc
     2401 ctcgagggcc gcacgtccgt ggcgaccctc gacccgggcg ttccgccccg caccccagga
     2461 gtaactcatc actctccccg ctcgtcgcac ccggcgacat cacccggcct ggtggcgcct
     2521 ccttccacga tcgtgtcttt tcggccttgc atccgcaagt gatcctgcac aaccatctgc
     2581 acgtgcatgt gggaaccaac agcgcccgat ggcgcaagag caccgcgagc aatccgagcg
     2641 gcaactgcgt tgagctggct gaactcgccg aagggatgat cggggtacgc aactcacgcc
     2701 acgcgaccgg ccccgtgctc gtctacaccc gggcggagat cgcggcgttc ttcgtggcag
     2761 tacgacacgg agagttcgac gatctcgcag gttgagcatc ccttcagacg gttgcgtcag
     2821 caaccggagc tcggcagagt gaatctcctc actaccctgc gcaagcgcga gcggcagtac
     2881 tcctgggggg agcgcggtgc ggcgatgaac ggtgcggcga cggtcagcgg gacgacgacc
     2941 agcgggacga cgatcgtgga gcagccatga gcgccgttcc cgaccacgtc gagcccgatg
     3001 gcagggtgcc cggcggcggt cccgaggacg ggcagccggg cagcatcatc cggggtgcgg
     3061 ccagcggtcc caccgtgctg cggatgggcc tcggcggcga gctgcgccgc aggcgggagg
     3121 cggcaggcgt cacccgcgag agagcgggcg aggcgatccg tgcgtctccc gcgaagatca
     3181 gcaggctgga gctcggtcgc gtcggtttca aggagcgtga cgtggccgac ctgctcactc
     3241 tctacggcgt cggcgagcag gacgagcgcg ccgagttcct cgcgctggcc cgtcgggcca
     3301 acgcgccggg gtggtggcac cggtacgccg acctgctgcc cagctggttc gagacctaca
     3361 tcggcctgga gcaggcggcg tctctcatcc ggacctacga gctgcagttc gtgccggggc
     3421 tgctccagac ccacgaatac gcacgtgccg tcacccgtct cggccacacc gaccccaccg
     3481 aggtcgagcg tcgcgtcgag ctgcggatgc ggcggcagga gatactcacc gcggccaacg
     3541 cgcccacgct ctgggcggtg atcgacgaag cggcgctgcg gcggaccctg ggcgggcccg
     3601 aactctcccg cgcgcagctc acccacctga tcgagatgaa cgagctgccg aacgtctccc
     3661 tgcagatcgc gccgctgcgc tccggcgggc acgccgccgc aggcgggccg ttcgccatcc
     3721 tgcggttcgc ggagccagac ctgcccgaca tcgtctacct cgagcagctc accagtgcgc
     3781 tgtacctcga caagcgcacc gacgtcgatc actacgccgc ggtcatggac cggctgggcg
     3841 cgcaggtcga cccgccggac cgcacggcgg acatcatcgc ccaagtccgc gccgagctct
     3901 gaaggccgca tccgcccccg taggctcggg cggtgcggcg caagctccgg tccccccttc
     3961 cgcagcggca cgggctcgac gccgtgcggc tgcggacgcc ggccgggaca tcatggacga
     4021 ccatgcgcga ccacctcgtg gagcggcttc cccgcgtcga cccggcccgc atcgacgcca
     4081 tgctgcgcga gcagcggatc gtcgaccggg acggaccgat agcacccgac gcgccgtacg
     4141 tcccggagtc gttcctctgg ttccaccggg acctgcccac ggaggtgccg gtgccgttcg
     4201 agatcgggat cgtgcaccgc gacgacgaac tcgtcgtggt ggacaagccg cacttcctgg
     4261 ccacgatccc acggggacag cacatcgtgg agaccgcgct cgtgcggctg cgtcgcgagc
     4321 tcggcctgcc cacgctcagc ccggcacacc ggctggaccg cgtcacagcc ggcctgctga
     4381 tgttcgtggt gcgccccgag cggcgcggcg cgtaccagac gctgtttcgc gaccgcctcg
     4441 tgcgcaagac gtacgaggcg gtcgcgcggt acgacccgca gctggcgctc ccccgcaccg
     4501 cgcgcagccg gatcgtcaag gagcgcgggg tgatctgcgc ccgtgaggaa cccggtccgg
     4561 tcaacgccgt gaccacggtc gagcttctgg aacaccgctc cgggccggac ggactgcggc
     4621 tgggccgcta ccggctcacc ccggagaccg gccgcaccca ccagctccgg ctccacatga
     4681 gcgcactcgg cgtgccgatt ctcggcgacg acttctaccc ggtgctcacc gagaagccgc
     4741 tcgacgactt ccggcgcccg ctgcaactgc tcgcctcaag cctggagttc accgaccccg
     4801 tcagcggcga ggagcgcagt ttccgtaccc ggatcgcgct gcagtccttc acgtcctacg
     4861 ccgcgtgggc cggatcgtgg tcaccctcgt aacgtgtcca ctgcggcacg ggagctgatg
     4921 tccgtccagc gccgcgtagg gacatccgag ctggtcacgc ggcattcttc ggaccgcgtg
     4981 ggcttccggg gcgggcacac acggcgcagg ttgtggcggc tgccgcgacg ccggcgcggg
     5041 tatccgcctc ctgggacgtc gaggcgcaga accgcactgt cctgagatcc tcttggcatg
     5101 acggcgagac cggcgggacg agcagcaccg accctggagg cggtagcggc gcgcgggcgt
     5161 gtcccgcgca acgcgggccg cgtgctcgcg ggctctacca cggtcggcga gcaggcgcgc
     5221 gaagcggtgc tgcaggccgc ggccgacctg tcgtacgtga ccaaccgcgc cgcgcgatcg
     5281 ctgatgacgc gccgcagcga ctcgatcgcg ttcgtcgtgg ccgagccgga ggagcggttc
     5341 ttcggcgacc cgcacttcgt gctggtcctg cggggcgccc acgggctgcg ccaggcgctg
     5401 gcctctcggc tgctcgccgt gatcgagcgg ggccgagcca ctcgagccga tggtgatgag
     5461 cactgagatc gtgtggcgcg agaccgcctg agcccacccg ttccggtctg agctccgcgg
     5521 gctgatggac gagatctcga ccgtgccgta atccgatctg agaggaccaa ttgaccacca
     5581 cgcaggctgc tccgtcgacc gatgcactgc ggttcccccc ggggttcctg tggggctccg
     5641 ccaccgcctc ctaccagatc gagggggccg tcgacgaggg cgggcgcacc ccgtccatct
     5701 gggacacgtt ctcccacacc ccggggcggg tcgagcgcgg caacaccggt gacgtcgccg
     5761 acgaccacta ccaccggttc cgcgaggacg tcgcgatcat ggcccggctg gggctcgcct
     5821 cctaccggtt ctccgtgtcc tggccgcgca tcacgccgca ggtcaccgcg gactcactcg
     5881 ggccggtcaa ccccgaaggc atcaggttct actccgacct cgtcgacgcg ctgctcgccg
     5941 tcggcatctc accgtcgatc accctttacc actgggacct gccccaatcc ctcgaggaca
     6001 cgggaggctg gacggcgcgg agcaccgcag agcggttcgg cgagtacgcc gaggtcgtcg
     6061 ccgcggcact gggcgaccgg gtgccgctgt tcatcacgct caacgagccg tggtgcagcg
     6121 cctacctcgg ctacgcgagc ggcgtgcacg ccccgggccg tacggacgac gccgccgcgc
     6181 tcacggcggt gcaccatctg aacctcgcgc acgggctggc ggccggatcg atccggcgcg
     6241 ccgcgcccgc gtcgcgggtg gcgctcacgc tcaacctcgc gtgggtgtgc ccggagaccg
     6301 actctccgca ggacgcggag gcggcgcggc gggtggacgg gctgcagaac cgggtgttcc
     6361 tcgaccccgt cctgcgcggc agctaccccg ccgatgtgca ggccgacacc gcggccgtga
     6421 ccgactggtc gttcgtgcgg cccggcgacc tggaggtgat ctcggcgccg ctggacgtgc
     6481 tgggcgtcaa ctactactcc cccacccggg tgcggcactg gacgcgggag cgccccaagg
     6541 agcacgccga cggccacggt gagggcaagg ccagcccgtg gatcgcgtgt gacgacgtgg
     6601 agttcccgtt ccagcacgac cgcaagaccg acatgggctg gagcatcgac ccgcgcggga
     6661 tgaccgagct gctgcggcgc gtggcccgcg aggcgccggg ttgcgagatc atggtcaccg
     6721 agaacggagc cgccttcccc gacgtggtga acggggacgg ccgcgtcgtc gacaccgacc
     6781 gcatcgacta cctccgcagc cacctggccg cggtgcacga agcgatcgag gacggggccc
     6841 cggtgacggg ctactacctc tggtcgctcc tcgacaactt cgagtgggcg tggggctacg
     6901 cgaagcgctt cgggatcgtc cacgtcgact acgacacgca ggcccggacg ccgaaggaca
     6961 gcgcattgtt ctatgccgac gttattcgga gaaacgccgt gccgagctga gacgactccc
     7021 cacgggatgc cccggccgtg agagcgcccg ggtagtcttg cggccggtgg ctcaccggcc
     7081 ggcaatagcc tcatgaacgg agctcggggt gcggctcggc gacgaggacg atctccgcaa
     7141 gggacctgat tccgaggacc gcgaccccaa ggacagcgac aaccacgacc gggacgcgca
     7201 ggaccgcgac agcgagcacc tccccatcga tgtcgccgcg ctacgagccg acgacgcact
     7261 ggtcgaagag ctggccgcgg ggctcgtcgc gctcggtgag ctgcaccacg gggcgcgcgg
     7321 ttccgacgac gagctggtgg ccatgctcgc ggcctgggtg gccgacgtcc ggccggacgc
     7381 ccagccaacg atcacggcca gcgcaaggga tacctccccg gatgagaagg ccctcggcgc
     7441 tccgtccctg gtctcgctgg tacgccccgc ctcggcgcac ggcagacacc gggctgccca
     7501 gtcggcttct gagcatggcg ccattccgta cgcccggcga ctcgctgtcg ccgccgccgt
     7561 gatcatgatg ggtacggcgg ggctggcagt cgggtcgtcc gacgcgtcac cgggtgaggc
     7621 cctctggtcc gtgtcgaagg tcttctacgc cgaacgtgcc cggtcggtcg aagccgccgc
     7681 cgaggtcaac acgggattcg agacggcgcg caccgcgctg cagcaagggc gtccagccga
     7741 cgccgcgcgg gcgatcgcga aggccagcgc gctgctcgac gaggtgcgcc cggccgaggg
     7801 ccgcgccgag ctcgcccatg agcaccagct gcttctcgcc gcgctcagcg gagagccctc
     7861 cggccagtcc gccgcgcaag ccccggtcgc acccgccccc cggcccccgg accagccggg
     7921 cacgagtagt accccggcgt ccacgccgac cccttccggt agccccgtgc tggcctcccc
     7981 accccttccc gccgagagtt ccaccgcgcc gcgggccgtc gcaccgccca ccggctcggg
     8041 cgcttctgcc gtcccatccg tctccggcgt ccccgataac gacacggccg gcccctcccg
     8101 ctccgggtcc gccagcacca ccgtgtccgt ggaggacggc acctccccct ccaccgcccc
     8161 ggcaacgccc ctccccggat ctgctcccca gccgccgacc ggacccactt ccccgacgca
     8221 gcccgagtcc cagccgcagg tggacccgtc gccgcagtct ccggtccaag ccggacccga
     8281 gccccagccg gaaccgcaac cccagccgca gagcgcgccc gagccccaga ctggatcggg
     8341 gccactgagc cagccccagt cggcgccggc cgccccgggt gacaccgaag gaccgggcca
     8401 gccaaccggg tcaggatcgg cgaacgagaa gaacggcggg agcggcggag cgggtgtcgc
     8461 caactccgcg accgacggcc ccgaaggcaa ccccgggcag ggtgaaccga gccagccatc
     8521 cgacagcggc ggtaactcgg acaacagcac taactcgaac aacagcggta actccgacaa
     8581 tagcggtggc cggaacaaca gtgagagcga cggcggcggc aactccgccg acggtgggaa
     8641 ctcggacggt ggcagaaact ccgacaacgg cagagggtcc ctgttgtcgc cggcgaccga
     8701 cctgatctcc tcgagtctca ggagtctccg gcagtcctag acggtacgtt cccccacgcc
     8761 accgatgcgg gggccggttt gccgcccgat gtaggcactg gtcacaccgt gccagagcgt
     8821 cagcgacgct aggtcgtggc ggcgtcggct cccgacccgg cagcggggcg ccgccgggac
     8881 acggccacca gcaccacgga agcggcgccg gcattgtgca gggcccgcag gccgagcccg
     8941 gtgaagcaca gcacagcgcc ggagcagaag cggcgtcggc cgcccgcccg gcactccagg
     9001 tcgatcgtcc cggactccac gaggaccagc gcgtcccacc attccgactc gtcgtagagc
     9061 cgtgtggtgc cgggcgcgag ggtgaccacg gtggtccgga acgacgggcc cagcggtcgg
     9121 ccgaggaacg agaacggagg cgtgtccatc acgaccgtcc tgcctgcgca gccccgacca
     9181 cgccgtccga cacaccgttc accgaaccct ccgcaccgtc gggcggaccg ctcgggccca
     9241 ctgccggtta cgacatccgc ggagggcgga aatcatcggc cgcggcggat ccggcctcgt
     9301 tctcgtcgag gtgcaaccgc agcatcgccg cagccgccca cgccggtggc ctgccgcgca
     9361 gcgatacgag caccatcgtt gcgaacgcca gcggcaccga ccacagggcc ggctgcccga
     9421 gcacgatcgc gacgaggccg ggtggggcgc cgaggaacag gtcgaccccg atcacgccgg
     9481 ccgaggagat cagcccgacg accatgccca cgacggcgcc ctgggtcgtc agccgcggcc
     9541 accagatccc gaggaccagc aacgggcaga aggtggacgc cgcgaccgcg aatgccgacg
     9601 tgaccagcac gcccacgtcg acccgcgccg cgggcagcgc gagcagcacc gccaccgccg
     9661 cagcggcgag cggcgtgaac cgcaggcgtc gcaacgtccc cggtaccagg tcgtgcgaga
     9721 tcgcaccgga cacggcgagc agcagcccga gggacgtcgc gaggaacgcc gcgaaggcgc
     9781 ccgcggtgag cagggcggtg aacagcgacc cgacccagct ctgatcgacc ctggccggca
     9841 gtgccaccac cacggtgtcc gtgccgccgg acaggtacag ctcggggagc aggaccgcgc
     9901 cgagcagccc gtagatcggc gggaagaggt agaacacccc gagcagcccg acggtgatgg
     9961 cagccgtgcg ccgggcgccg tggccgtcgg cactggtgtg gaaccggacg aggatgtgcg
    10021 gcaggcccat cgtcccgaac acggtggcga tcagcaccga ccacgtggcg agcaccgggt
    10081 acccaccggg gtcgagcagg ggccgttccc actccctgcc gccaggcggc cgcccgcctc
    10141 cgacctgcgg cacggccgcc ccggcgtcga acacccagct ggtcccggcc tgcgcttggt
    10201 agcgtccggg agggagcacc tgctgcggcc cgcccccgat ggacacgcgg gtcggctcgg
    10261 tgatcgtgag ctcggtgtcg agccggaact gcacctgggt ggctgcggag aaccgtgtga
    10321 actcgaccgg ctcgagagcg ttggcgcggg tgctcgggcc cacgtgcagc accagccaga
    10381 tcgccg
//
LOCUS       DASRSC010000315        12273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3244971,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000315 DASRSC010000000
VERSION     DASRSC010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12273
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3244971"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VFE39_15520"
     CDS             complement(<1..209)
                     /locus_tag="VFE39_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET6260045.1"
                     /translation="MTSMWGAPLRDRWGRAWRRDPRQARFLTIASLCWVLRNRAYTPW
                     YLVRYWRLLRFRLANPHVILRGMVFL"
     gene            268..519
                     /locus_tag="VFE39_15525"
     CDS             268..519
                     /locus_tag="VFE39_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260046.1"
                     /translation="MTTERRDGSEPTSRTEEDRVVSRRVDNPTAAGPEDERAYRAADP
                     GPDAEPGGDGPASEGHGSTTERAEYDLDAADESEPTRSE"
     gene            583..1419
                     /locus_tag="VFE39_15530"
     CDS             583..1419
                     /locus_tag="VFE39_15530"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HET6260047.1"
                     /translation="MSRIAVVGSVNLDLVARVSRLPAPGETLAATGLSRVPGGKGANQ
                     ALAARRLGAEVALVAAVGDDPAADEALALLRRDGVRLDGLRREPAHPTGHALITVDDS
                     GETTIVVAAGANATLQVTADDVRGADAVLTVLEVPDSAVAAAAEHATGLFVLNAAPAR
                     AVAPELLRRVDLVIVNSAEFAAIEGLDAAGAVAVTSGGDGAVLRRGGREVARAEPPPV
                     TVVDGTAAGDTFTAALLVALLEGASDADALRSACAAGALAVTRAGAQPSLPTAAELAA
                     VL"
     gene            1416..2354
                     /locus_tag="VFE39_15535"
     CDS             1416..2354
                     /locus_tag="VFE39_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HET6260048.1"
                     /translation="MTHPKPLIIDTDPGVDDAVAIMLALASPEVELRAVTTVFGNVPL
                     PRTTENAGRLLALCDRSDVPVGAGAARPLVHPQRERAEAWHGGDGLGGRAEALPAPIV
                     EPDPRGAVGLMASVLRAAGEPVTIAPIGPLTNVALLLAVHPELAPRIDRIVVMGGALG
                     AGNTSGVAEFNVHCDPEAAHRVFTQADVPVTMVPLDLTLRCPAGPEWLDELASGGPRC
                     AELARVIVRYRAAYRTRYGIDAVALHDAVAVLEAMLPGTLATTAMPIQVACDLGPARG
                     ATVAVPGAHGAPVHVALDADPDPVLAEILARLLRLS"
     gene            complement(2409..3056)
                     /locus_tag="VFE39_15540"
     CDS             complement(2409..3056)
                     /locus_tag="VFE39_15540"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6260049.1"
                     /translation="MSSAVVTGPPVTTVLVGQPPALMCAAHALARRGIELLATVAPRD
                     AVRLVSLHRPQVVVVNLDGGQAALTVIATVTATVQQIPVLTLTAVPDHATVLAAVQAG
                     AVSHLVAPGNADELADAVRCTAAGQAVFSPGLADVVLEQFGRPVDPQEGARRLTQREA
                     DVLRLVVDGLTARQIAARLVLSPRTVENHVQNVLRKLGLRSRAALVRYAIENGLA"
     gene            complement(3169..3834)
                     /locus_tag="VFE39_15545"
     CDS             complement(3169..3834)
                     /locus_tag="VFE39_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6260050.1"
                     /translation="MTDELDEITVMVVDDHPIWREGVARDLTERGLSVVATAPDAEAA
                     VRIALATRPRVVLMDLNLGVTSGVRAIEGILEKLPETRILVLSASGEHTDVLEAVKAG
                     ATGYLLKSAGADELLAAVRRTADGFPVFTPGLAGLVLGEYRRLASEPEGGDGAAPVLS
                     ERETEVLRLVAKGLTARQIGERLVLSHRTVESHVQNTLRKLQLHNRVQLVRYAIEQGL
                     TDS"
     gene            complement(3831..4985)
                     /locus_tag="VFE39_15550"
     CDS             complement(3831..4985)
                     /locus_tag="VFE39_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5931 domain-containing protein"
                     /protein_id="HET6260051.1"
                     /translation="MTPPSATETEQPLAPLWRGLLVYRVLTLLSAAAVVLYSLNAYAV
                     PAGAVAVLAVMVAWTVATGYGYLGGLPGATDRRGHVAIADVVVTVGVMATTPLVQTPA
                     QFAADAPVMGSIWTPGAALACALAYGVRGGLAGALALSAALLAFQAHWRAEVGDIQLL
                     LLVGVTIGFASTVLRRSAERLRHAVAAEAGMRERERLARAVHDGVLQVLGYVRRRGAE
                     LGGAAAELGVLAGEQEFALRTLLTTGAAPVDVHGRRDLAAALRMLASEYVSVSTPAHR
                     VELPAHVVDELVAVAGAALTNVAEHVGPDAPAWVLLEETGDGVEICVRDEGPGIPDGR
                     LATAEAEGRLGVARSMRGRVHDLGGTISCDTGPGRGTDWTISVLKRGVGA"
     gene            complement(5029..5985)
                     /locus_tag="VFE39_15555"
     CDS             complement(5029..5985)
                     /locus_tag="VFE39_15555"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6260052.1"
                     /translation="MSTDPAPNPHATAEQVAAARHDRKLANVLYHDWEAGSYDEKWSI
                     SYDERCITYAADRFRLVAGEGSRPYEHALELGCGTGFFLLNLMQAGVAKRGSVTDLSP
                     GMVQAALRNADGLGLDVDGRVADAELIPYEDATFDLVVGHAVLHHIPDVELALREVLR
                     VLKPSGRFVFAGEPTTVGDAYARRLGALTWKAATTITKLPALQSWRRPQEELDESSRA
                     AALEAEVDIHTFDPAELEEIARRAGARDVKVVTEEFTAAMLGWPVRTFEAAVPPGKLG
                     MRWAMFAYRGWQRLSWLDRNVLARVVPRGWFYNALVTGTRIP"
     gene            complement(6001..6780)
                     /locus_tag="VFE39_15560"
     CDS             complement(6001..6780)
                     /locus_tag="VFE39_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HET6260053.1"
                     /translation="MSEFVNLEVDSGVGTIRLDRPPMNAFSRQVQEELKACAEEATRR
                     ADVRAVVVYGGEKVFAAGADIKEMIGMSYGDIAPVARRLSACFGSISEIPKPTVAAIT
                     GYALGGGMEVALGCDRRVCGDNAKLGQPEILLGIIPGGGGTQRMARLIGAPRAKDLIF
                     TGRMVGAEEALAIGLVDQVVPADEVYTAARSWAEQFVDGPAVALAAAKKAIDGGLDVD
                     LRTGLDLEAELFAALFATEDRQAGMTSFVENGPGKAQFTGR"
     gene            complement(6846..7610)
                     /locus_tag="VFE39_15565"
     CDS             complement(6846..7610)
                     /locus_tag="VFE39_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6260054.1"
                     /translation="MQGVAVRRSTSTLLRDVDWAVELDERWVVLGPNGAGKTTLLRLA
                     AAELHPTTGSVDVLGERLGRVNVFELRTRIGLTSASLGMRVPGDEVVRDVVVSAGYGV
                     LGRWRERYDATDTDRAEQLLDALGVRGLAERTFGTLSEGERKRTLIARALMTDPELLL
                     LDEPAAGLDLGGREDLVGRLAKLAADPDAPASVLVTHHVEEIPPGYSHGLLLRGGRVV
                     AAGLLEDVLTDENLTATFGLPLKVQRRRGRYTAWLR"
     gene            7766..9775
                     /gene="glgX"
                     /locus_tag="VFE39_15570"
     CDS             7766..9775
                     /gene="glgX"
                     /locus_tag="VFE39_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET6260055.1"
                     /translation="MPVFPLGAHADGRGVRFAVASTSADAVEVCLVDGPDGALSEKRV
                     SLTERTFGVWHGYIDGVTPGQRYGYRVHGPYRPWDGLRANPAKILVDPYARQITGRVT
                     DLHAARGWVDDPMTGTLNTVDSLGHVPLSVVTGPDVPAAGLLLPEVPWSETVIMELHV
                     RGYTKLHPEVPPEHRGTYLGLAHPAVVEHLRALGVTAVELLPVASIAEEPPLLQSGRT
                     NYWGYATLGFLAPHAGYASVPGAEIEEFRTMVSALHAAGIEVILDFVPNHTAEGGVGG
                     TTLSYRGLDAPAYYSLGGNGRDADITGTGNTLDAGSPTVIRLVCDAMRHWVTVFGVDG
                     FRIDLASVLGRPRSGAFDPHGPLLTAIATDPVLSRVKLIAEPWDATGEGYRVGGFGVA
                     WSEWNGRYRDAVRDFWRGHGGIGEVASRLTGSSDLYRLSGRRPWASVNFVTAHDGFTL
                     RDLVSYERKHNEANGENNRDGTDDNRSQNFGVEGETSSPVFGSRRLATARAMLGTLLL
                     STGTPMLLGGDELWRTQRGNNNAYCLDDETSWVDWTDDDDAASLTAFTARVAEIRRSS
                     PALHRDRFYRDGEVLWWHPSGRRIGGHDWHDSRLRTFGLLRAEWLLLLHAGDQAVPAT
                     LPPGGPFTPVLDSTRPDGEPVSRRTLPGGTTITLQPRSLLLLRTP"
     gene            9843..10070
                     /locus_tag="VFE39_15575"
     CDS             9843..10070
                     /locus_tag="VFE39_15575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6260056.1"
                     /translation="MPRYDFRCRECGSTFEVSRPMSAASDPAACPAGHDDTVKLLTTV
                     AVGGLAPRAGSRAAAPAAAAGGCCGGGCCGG"
     gene            complement(10100..11425)
                     /locus_tag="VFE39_15580"
     CDS             complement(10100..11425)
                     /locus_tag="VFE39_15580"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6260057.1"
                     /translation="MKTATADISVIGTATTQRQGTFAALRVRNFRIYLAGQSVASTGT
                     WMQSIALDWLVLELTHSASAVGITMALQFLPMLLLGMYGGMIADRYPKRAILMVTQSL
                     NGALSAGLAVLTITGHVRVEYVYLFALAAGLVFVVDNPTRQVFVNEVVPAPYVRNAIA
                     LNAAVFQTTRLLGPAVSGVLIGTVGTGWAFAANSASYLAPLIGLLLIRSADLVRPPTV
                     VRAPGQLRSALRYVAARPHVGWTILLVGVIGTFGLNFPVVLTAMADSTFHGGANLYGL
                     FNVVLALGSVAGALIAGGRTRTRLRTLVVMAMVFGLAQVAAALAPGLGTFLGALVALG
                     MVNLALQAMANSSVQSWVDPAVRGRVMGLYMLAFVGGTPLGGPTIGWITSTFGPRAGM
                     AVCGLVPMIAAVGVAVALRRKAVNEPQIARPEPVAEAVIAPRIAQPEAA"
     gene            11531..>12273
                     /locus_tag="VFE39_15585"
     CDS             11531..>12273
                     /locus_tag="VFE39_15585"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6260058.1"
                     /translation="MTVTYDPAQLETFLAVAQTRSFTQAAARLGIRQPTVSQHVRKLE
                     EATGRVLVQRDTHTVTITTDGEAMIGFARSILAAHDHAAGYFSGQRPQGRLRIGMSDD
                     LALTRLPQILRDFRQDNPLVDLDLTIDQSGTLHRRLENDRLDLFVGKRPRGEHRGQLV
                     RRDRLVWVGNAATRVEFSRPLPLVVYPPPSLSRVAMQNALERTNTAYRSACICRGVNG
                     LIAAVAAGIGISALAGSLVPVQLTTLGPQ"
BASE COUNT         1765 a         4661 c         4252 g         1595 t
ORIGIN      
        1 aggaacacca tgccgcgcag gatcacgtgg gggttcgcca gccggaaccg cagcagccgc
       61 cagtaccgga ccaggtacca gggggtgtag gcgcggttgc ggagcaccca gcacagcgag
      121 gcgatcgtga ggaagcgggc ctgccgaggg tcgcggcgcc aggcccgtcc ccagcggtca
      181 cgcaggggcg cgccccacat gctggtcatg gatcgacgat agggcggggg cgtttcaccg
      241 gcgctcccac ccgggtagcc cgctgccgtg accaccgaga gacgcgacgg cagcgaaccg
      301 acgagtcgca ccgaggagga ccgggtcgtg tcccggcggg tcgacaaccc gacggccgcc
      361 ggtcccgagg acgaacgggc ctaccgcgct gccgaccccg gacccgacgc cgaaccgggc
      421 ggggacggcc cggcgagtga gggtcacggg agcaccaccg agcgcgctga gtacgacctg
      481 gacgccgccg acgagagcga gccgacccgt tcggagtgat ccagcgctct cacgagaccc
      541 acgctcttgc gcccgcccgc gtcaccgacc atcgtccgcg aggtgtcccg gatcgcggtg
      601 gtcggcagcg tcaatctcga tctcgtggcc cgggtgtcgc ggctgcccgc gcccggggag
      661 acgctggccg cgaccgggct gagccgggtg cccggcggga agggcgccaa ccaggcactg
      721 gcagcccgca ggctcggggc cgaggtggcc ctggtcgctg cggtcggcga cgatcccgct
      781 gccgacgagg ccctcgccct gctgcggcgc gacggcgtac ggctcgacgg gctgcgccgg
      841 gagcccgcgc acccgaccgg acacgccctc atcacggtgg acgactccgg cgagaccacg
      901 atcgtcgtcg ccgccggggc gaacgcgact cttcaggtga ccgcggacga cgtccgggga
      961 gcggacgccg tcctcaccgt cctggaggtc ccggacagcg cggtggcggc cgccgctgag
     1021 cacgcgaccg ggctgttcgt gctcaacgcc gctcccgcgc gggcggtcgc acccgagctg
     1081 ctccggcggg tcgacctggt gatcgtcaac agcgcggagt tcgccgcaat cgagggcctt
     1141 gacgcggcag gtgccgtcgc cgtcacctcc ggcggggacg gcgcggtgct gcgccgcggc
     1201 ggccgggagg tggcccgtgc cgagccgccg ccggtgaccg tcgtcgacgg caccgcagca
     1261 ggtgacacgt tcaccgccgc gctgctggtg gcgctgctgg agggcgcctc cgacgccgac
     1321 gccctgcgca gtgcgtgcgc ggccggggcg ctggccgtga cacgcgccgg cgcgcaaccc
     1381 tccctgccca ccgcagccga gctggcggcc gtcctgtgac gcacccgaag ccgctgatca
     1441 tcgacaccga ccccggagtg gacgacgcgg tcgcgatcat gttggcgctg gcctcgccgg
     1501 aggtcgagct gcgggccgtg acaacggtgt tcggcaacgt cccgctgccc aggaccaccg
     1561 agaacgcggg gcggctcctg gcgctgtgcg accggtccga cgtgccggtg ggcgcaggtg
     1621 ccgcgcgtcc gctcgtgcac ccgcagcgcg agcgcgccga ggcgtggcac ggcggtgacg
     1681 gactcggcgg gcgggccgag gcgctgcccg ccccgatcgt cgagccggac ccgcgcggag
     1741 ccgttgggct gatggcatcg gtgctgcgtg cagcaggcga accggtgacc atcgcaccga
     1801 tcgggccact gacgaacgtg gcgctgctgc tcgccgtgca cccggagctg gcgccccgca
     1861 tcgaccggat cgtggtgatg ggcggcgccc tcggtgccgg caacacgagc ggtgtcgccg
     1921 agttcaacgt ccactgcgac cccgaggcgg cccaccgggt gttcacccag gccgacgtgc
     1981 ccgtgacgat ggtgccgctc gacctcaccc tgcgctgtcc cgccggcccc gagtggctcg
     2041 acgagctggc atccggtggc ccccgctgcg ccgagttggc tcgggtgatc gtccgatacc
     2101 gcgcggcgta ccgcacccgg tacggcatcg acgccgttgc gctccacgac gccgtcgccg
     2161 tgctggaggc gatgctgccc ggcacgctgg caacgacggc gatgccgatc caggtggcgt
     2221 gcgatttggg gccggcccgg ggcgccaccg tcgcggtgcc gggcgcccac ggggcacccg
     2281 tccacgtcgc cctcgacgcc gatccggacc ccgtgctggc cgagatcctg gcccgcctgc
     2341 tccgcctgag ctgagaagct cgtgcgcgga tacgagtgct ggagcactcg tatccgcgca
     2401 cggggccgtc aggccagccc gttctcgatc gcgtagcgca ccagtgcggc gcggctgcgc
     2461 aggcccagct tgcgcaggac gttctgcacg tggttctcca cggtgcgcgg ggagaggacc
     2521 agccgggcgg cgatctggcg cgcggtgagc ccgtcgacca ccagccggag cacgtccgcc
     2581 tcccgctggg tgagccgccg cgcaccctcc tgcggatcca ccgggcgccc gaactgctcc
     2641 agcaccacgt cggcgaggcc ggggctgaag accgcctgcc cagctgcggt gcagcgcacg
     2701 gcgtcggcca actcgtccgc gttgccgggc gcgacgaggt ggctcacggc acccgcctgc
     2761 acggcggcga gcacggtcgc gtggtcggga acggcggtga gggtgagcac cgggatctgc
     2821 tgcacggtcg cggtgaccgt cgcgatgacc gtgagcgcgg cctggccccc gtcgaggttc
     2881 accacgacca cctgcggacg gtgcagcgag accagccgga cggcgtcccg gggagcgacc
     2941 gtggccagca gttcgatccc acgacgggcc aacgcgtgcg ctgcgcacat gagtgcggga
     3001 ggttggccga cgagcacggt ggtgaccggc ggaccggtca ccaccgcgct gctcatccgc
     3061 cccccgtccg gcgcatgatc atggcgcgga tcctcccaag gagttacgcc acgcggaaac
     3121 cgttcggaga tctccacccg ttcgccacga cgtgatgcca tatcgtgatc aactgtcggt
     3181 caggccctgc tcgatggcgt agcggaccag ctgcacgcga ttgtgcagct gcagcttgcg
     3241 cagcgtgttc tgcacgtggc tctccaccgt gcgatgcgac agcacgagtc gctccccgat
     3301 ctgccgcgcc gtgaggccct tggccaccag ccgcagcacc tcggtctccc gctcgctgag
     3361 cacaggcgcc gcgccgtcgc ctccctccgg ctcgctcgcc agccgccggt actcgccaag
     3421 gaccagcccg gccaggcccg gggtgaacac cgggaagccg tcggccgtgc gccgcacggc
     3481 tgcgagcagc tcgtcggccc ccgccgactt gagcaggtac cccgtggccc ccgccttcac
     3541 cgcctccagc acgtcggtgt gctcgccgct cgccgagagc acgaggatcc gggtctcggg
     3601 cagcttctcc aggatcccct cgatcgcccg tacgccggag gtcacgccga ggttgagatc
     3661 catgagcacc acgcgcggcc gggtcgcgag cgcgatccgc accgcggcct ccgcgtccgg
     3721 ggcggtggcg acgacgctca ggccgcgttc ggtcaggtcg cgggccacgc cctcccgcca
     3781 gatggggtgg tcgtccacca ccatcacggt gatctcgtcg agctcgtccg tcatgctcct
     3841 accccccgct tcaggacgct gatggtccag tccgtgcccc gacccggtcc ggtgtcgcag
     3901 gagatcgtgc cacccaggtc gtgcacccgg ccgcgcatcg accgggccac gcccagccgc
     3961 ccctcggcct ccgcggtggc cagcctgccg tcggggatgc caggcccctc gtcgcgcacg
     4021 cagatctcca cgccgtcccc cgtctcctcc agcaggaccc atgcaggcgc gtccggcccg
     4081 acatgctccg cgacgttcgt cagcgcggca cccgccaccg ccacgagctc gtcgacgacg
     4141 tgcgcgggca gctcgacgcg gtgcgcaggg gtcgacacgc tgacgtactc ggaggcgagc
     4201 atccgcagcg cggcggcgag gtcgcggcgc ccgtgcacgt cgacgggcgc cgctccggtg
     4261 gtcagcagcg tgcgcagcgc gaactcctgc tccccggcga gcacgccgag ctccgcggcc
     4321 gccccgccca gctcggcacc ccgccgccgc acgtacccga gcacctgcag cacaccgtcg
     4381 tgcaccgcgc gggccagccg ctcccgctcg cgcatccccg cctcggcggc gaccgcgtgc
     4441 cggagccgct cggccgagcg gcgcagcacc gtggacgcga agccgatcgt caccccgacg
     4501 agaaggagca gctggatgtc gcccacctcg gcgcgccaat gcgcctggaa ggcgaggagc
     4561 gcggccgaca gggcgagcgc ccccgccagc ccacctctga ctccgtaggc gagcgcgcag
     4621 gccagggccg cccccggcgt ccagatcgac cccatcacgg gcgcgtcggc ggcgaactgc
     4681 gcaggtgtct gtacgagcgg tgtggtcgcc atgaccccga cggtcaccac gacgtcggcg
     4741 atcgcgacgt ggccgcgccg gtccgtcgcc cccggcaggc cgcccaagta gccgtagccg
     4801 gtggccaccg tccaggcgac catcaccgcg agcacggcga cggcgccggc aggcacggca
     4861 tacgcgttga gcgagtacag gacgacggcg gccgcactga gcagcgtcag cacccggtag
     4921 accagcagac cgcgccacag cggtgcgagc ggctgctccg tctcggttgc cgacggcggg
     4981 gtcaccgccc catcctggcc gacgggcacc gcacccggat cagtaggact acgggatcct
     5041 ggtgcccgtg acgagcgcgt tgtagaacca cccacgtggc acgacgcggg caagcacgtt
     5101 ccggtccagc caggacagcc gctgccagcc gcggtaggcg aacatggccc agcgcatccc
     5161 gagcttcccc ggtggcacgg cggcctcgaa cgtgcgcacc ggccagccga gcatcgccgc
     5221 cgtgaactcc tcggtgacga ccttgacgtc gcgcgccccc gcccggcgcg cgatctcctc
     5281 gagctcggcc ggatcgaacg tgtggatgtc gacttctgcc tccagcgcgg ccgcacggga
     5341 cgactcgtcg agctcctcct gcggccgccg ccagctctgc agcgccggca gcttcgtgat
     5401 ggtcgtggcc gccttccagg tgagcgcacc gagcctgcgc gcgtacgcgt caccgaccgt
     5461 tgtcggctca cccgcgaaca cgaaacgccc gctcggcttc agcacccgca gcacctcgcg
     5521 gagcgccagc tcgacgtcgg ggatgtggtg cagcacggcg tgcccgacga ccaggtcgaa
     5581 cgtcgcgtcc tcgtaaggga tgagctcggc gtcggccacc cggccgtcga cgtccaggcc
     5641 gagcccatcg gcgttgcgca gggcggcctg caccatgccc ggcgagaggt cggtgaccga
     5701 cccccgcttc gcgaccccgg cctgcatcag gttgagcagg aagaaccccg tgccgcaacc
     5761 gagctccagc gcgtgctcgt agggccggct gccctcgccg gccaccagcc ggaagcgatc
     5821 ggcggcgtag gtgatgcagc gctcgtcgta ggagatcgac cacttctcgt cgtacgagcc
     5881 cgcctcccag tcgtgataga ggacgttggc cagcttccgg tcgtgccggg cggccgccac
     5941 ctgctcagcg gtggcatgcg ggttcggtgc cgggtccgta ctcatgggta gggagggtaa
     6001 tcagcggccc gtgaactggg ccttgcccgg cccgttctcg acgaacgacg tcatgccggc
     6061 ctgccggtcc tcggtggcga agagcgccgc gaagagctcc gcctccaggt ccagaccggt
     6121 gcgcaggtcg acgtcgagcc cgccgtcgat tgccttcttg gccgccgcca gcgccaccgc
     6181 gggaccgtcg acgaactgct ccgcccagct ccgggccgcg gtgtacacct cgtcggccgg
     6241 cacgacctgg tcgacgaggc cgatggccag cgcctcctcc gccccgacca tccggccggt
     6301 gaagatcagg tccttggccc gcggcgcccc gatcagccgg gccatgcgct gggtgccgcc
     6361 accgccgggg atgatcccca agaggatctc cggctggccg agcttcgcgt tgtcgccgca
     6421 gacccgccgg tcacacccca gcgcgacctc catcccgccg ccgagggcgt agccggtgat
     6481 cgccgcgacc gtcggcttcg ggatctccga gatcgacccg aagcaggccg agagcctccg
     6541 cgcgaccggt gcgatgtccc cgtaggacat gccgatcatc tccttgatgt cggcgcccgc
     6601 cgcgaacacc ttctccccgc cgtacacgac cacggcccgg acgtcggcgc gccgcgtggc
     6661 ctcctcggca cacgccttca gctcctcctg tacctgcctg ctgaacgcgt tcatcggcgg
     6721 ccggtccagc cggatcgtgc ccacaccgga gtcgacctcg aggttcacga attcgctcac
     6781 gaccgcaccc taacccggcc ctccggcccg ccctcgcggc gaacgaggag ccgggacggc
     6841 gcgggtcagc gcagccaggc cgtgtaccgg ccgcggcggc gctgcacctt gagcgggagc
     6901 ccgaaggtcg cggtcaggtt ctcgtcggtc agcacgtcct cgagcagccc ggcggcaacc
     6961 accctgcccc cgcggagcag caggccgtgg ctgtagccgg gcgggatctc ctccacgtgg
     7021 tgggtgacca gcaccgacgc cggcgcgtcc gggtcggcgg ccagtttcgc gagccgcccg
     7081 acgaggtcct cgcggccacc gaggtcgagc ccggcggcgg gttcgtcgag caacagcagc
     7141 tctgggtcgg tcatcagcgc ccgcgcgatc agcgtgcgct tgcgctcgcc ctccgagagc
     7201 gtcccgaacg tccgctccgc caggccacgg acacccagag cgtcgagcag ttgctccgcc
     7261 cgatcggtgt cggtggcgtc gtagcgctcc cgccagcgac ccagcacgcc gtaccccgcg
     7321 ctcaccacga cgtcacgcac cacctcgtcg cccgggaccc gcatccccag cgacgccgag
     7381 gtgagcccga tccgggtgcg cagctcgaac acgttgaccc gcccgagccg ctcccccagc
     7441 acgtccaccg agcccgtcgt cgggtgcagc tccgccgcgg ccagccgcaa gagggtggtc
     7501 ttgcccgcgc cgttcggccc cagcaccacc cagcgctcgt ccagctccac agcccagtcg
     7561 acgtcacgca gcagcgtcga cgtggaccgc cgcaccgcca cgccctgcat ccggacgacg
     7621 aggtcggtgc tgtcggcaac gagtccgtca ccacgagcga caccatcgcg cgcagcatca
     7681 gcgcgccccc cgccggcgct acgtgcggtg agtaaagact cagattgcga ttccagcgct
     7741 gccacgggct ctatcctcgc cgatcgtgcc ggtcttccca ctgggtgccc atgccgacgg
     7801 ccgtggggtg cgcttcgccg tggcctcgac cagcgccgac gccgttgagg tatgcctcgt
     7861 cgacgggccg gacggggccc tgagcgagaa gcgggtgtcg ctcacggagc gcacgttcgg
     7921 cgtctggcac gggtacatcg acggggtgac gcccgggcag cgctacggct accgcgttca
     7981 tggcccgtac cggccgtggg acgggctgcg ggccaacccg gccaagatcc tcgtcgaccc
     8041 ctacgcccgg cagatcaccg gtcgcgtcac cgacctgcat gcggcccgcg gctgggtcga
     8101 cgacccgatg acgggcacgc tgaacaccgt cgactcgctc ggtcacgtgc cgctctccgt
     8161 cgtcaccggg cccgacgtcc ccgcggcggg cctgctgctg cccgaggtgc cgtggtcgga
     8221 aaccgtgatc atggagctgc acgttcgcgg ctacacgaag ctgcaccccg aggtgccgcc
     8281 cgagcaccgc ggcacctacc tcgggctcgc ccacccggcc gtggtcgagc acctgcgcgc
     8341 gctcggcgtc acggccgtcg aactgctgcc cgtggcatcc atcgccgaag agccgccgct
     8401 gctgcagagc ggccggacga actactgggg ctacgccaca ctcggcttcc tcgctccgca
     8461 cgccgggtac gcgagcgtcc cgggcgcgga gatcgaggag ttccgcacca tggtcagcgc
     8521 gttgcacgcc gccgggatcg aggtgatcct cgacttcgtc cccaatcaca cggcggaggg
     8581 cggggtgggc ggcacgacgc tctcctaccg cggcctggac gcccccgcgt actactcgct
     8641 cggcggcaac ggccgcgacg ccgacatcac cggcaccggc aacaccctcg acgccggctc
     8701 gcccaccgtg atcaggctgg tgtgcgacgc gatgcggcac tgggtcaccg tcttcggtgt
     8761 cgacggcttc cggatcgacc tcgccagcgt gctcggccga ccgcgctcgg gggcgttcga
     8821 cccccacggg ccgctgctga ccgccatcgc gaccgacccg gtgctgtccc gggtcaagct
     8881 catcgccgag ccgtgggacg ccaccggcga gggctaccgg gtcggtggct tcggcgtggc
     8941 ctggtcggag tggaacggcc gctaccgcga cgcggtgcgc gacttctggc gcggccacgg
     9001 cggcatcggc gaggtggcgt cccgcctgac cggcagctcc gacctgtacc ggctctcggg
     9061 acggcggccg tgggcgtcgg tcaacttcgt cacggcacac gacggtttca cgctgcgtga
     9121 cctcgtgtcc tacgagcgca agcacaacga ggccaacggc gagaacaacc gcgacggcac
     9181 cgacgacaac cgctcgcaga acttcggcgt cgagggcgag acgtcctcgc cggtgttcgg
     9241 gtcccggcgg ctcgcgaccg ctcgcgcgat gctcggcacg ctcctgctgt cgaccggaac
     9301 cccgatgctg ctggggggtg acgagctctg gcgcacccag cgcggcaaca acaacgccta
     9361 ctgcctcgac gacgagacgt cctgggtgga ctggaccgac gacgacgacg cggcctcgct
     9421 cacggcgttc accgcgcggg tggcggagat ccggcgcagc tcccccgccc tgcaccgcga
     9481 ccgcttctac cgcgacggcg aggtgctctg gtggcacccg tcggggcgcc ggatcggtgg
     9541 acacgactgg cacgacagcc gcctgcgcac cttcgggctg ctgcgcgcgg agtggctgct
     9601 gctcctacac gccggcgacc aggcggtgcc cgccacgctg ccccccggcg ggccgttcac
     9661 gccggtgctg gacagcaccc ggccggacgg agagccggtc agccgccgca cgttgcccgg
     9721 gggcaccacc atcacgctcc agccacgctc gctactgctc ctccgcactc cctgacccca
     9781 gcccgcgcga tgtgtccatg tggcaggtgg gatcggcggg ggtgaggacg taggctctcg
     9841 ctgtgccccg ttatgacttc cggtgtcgtg agtgcggctc gacgttcgag gtcagccgtc
     9901 cgatgagcgc cgcgagcgac ccggccgcct gcccggccgg ccatgacgac acggtcaagc
     9961 tgctcaccac cgtcgccgtc ggcgggctag caccccgggc gggctcgcgc gccgcagccc
    10021 cggcggctgc cgccggcgga tgctgcggcg gcgggtgctg cggcggctga gccccttcct
    10081 cggcctgcag gcgcggccgc tacgccgcct ccggctgtgc gatccggggc gcgatcaccg
    10141 cctccgcgac cggttccggc cgcgcgatct ggggctcgtt caccgccttc cgccgcagcg
    10201 ccaccgcgac cccgaccgcg gcgatcatcg gtacgaggcc gcagacggcc atgcccgccc
    10261 gcggtccgaa cgtcgacgtg atccagccga tcgtcggccc gcccagcggc gtcccgccga
    10321 cgaacgcgag catgtaaaga cccatcacgc ggcctcggac cgcggggtcc acccaggact
    10381 gcaccgacga gttggccatg gcctgcaagg cgaggttgac catcccgagt gcgacgagcg
    10441 cgccgaggaa ggtgccgagt ccgggcgcca gcgcggcggc cacctgggcg agcccgaaca
    10501 ccatcgccat gaccacaagc gtgcgcaggc gagtgcgtgt ccggccccct gcgatcaggg
    10561 cgccggccac cgagcccagt gccagcacca cattgaacaa gccgtagagg ttggcgccgc
    10621 cgtggaacgt cgagtcggcc atcgccgtca ggaccacggg gaagttcagc ccgaaggttc
    10681 cgatcacccc gacgagtaag atcgtccatc ccacgtgcgg acgcgcggcc acataccgca
    10741 gggccgaacg gagctggccg ggggcgcgca ccacagtggg ggggcgcacc aggtcggccg
    10801 aacggatgag cagcagcccg atcaacgggg ccaggtagga ggccgagttc gcagcgaacg
    10861 cccagccggt gcccaccgtg ccgatcagca cacccgagac ggcgggaccg agcagccggg
    10921 tggtctggaa gaccgctgcg ttcagcgcga tcgcgttgcg cacgtagggc gccggcacca
    10981 cctcgttgac gaacacctgc cgggtcgggt tgtcgaccac gaacaccagc cccgcggcga
    11041 gcgcgaacag gtagacgtac tcgacgcgga catggccggt gatcgtcagg acggcgagcc
    11101 ccgcgctcag cgccccgttc agcgactggg tgaccatcag gatcgcccgc ttcgggtagc
    11161 ggtccgcgat catgcccccg tacatcccga ggagcagcat cggcaggaac tgaagcgcca
    11221 tcgtgatgcc gacggccgac gcgctgtgcg tcagctccag caccagccag tccagagcga
    11281 tgctctgcat ccacgtgcct gtgctcgcga cgctctggcc ggcgaggtag atgcggaagt
    11341 tccggacgcg cagcgcggcg aatgtgccct ggcgctgcgt cgtcgcggtc ccaatgacgg
    11401 atatgtcggc cgtcgccgtt ttcacagaaa gccttccgta gcgcggaaaa aaaatcaacc
    11461 tcatccagtc tggaggagaa cgcgctattc tgaacgccga tcagccgact aactgttatc
    11521 gcgaaggggt atgaccgtca cctacgaccc ggcacagctc gagacgttcc tcgccgtcgc
    11581 ccagacccgg agtttcaccc aggcggctgc ccggctgggc atccgccagc cgaccgtcag
    11641 ccagcacgtc cgcaagctgg aggaggccac ggggcgagtg ctggtgcagc gcgacacgca
    11701 caccgtcacg atcaccacgg acggcgaggc catgatcggc ttcgcgcggt cgatcctcgc
    11761 ggcccacgat catgccgcgg gctacttcag cgggcagcgc ccgcagggca ggctgcggat
    11821 cggcatgtcc gacgacctgg cgctcacgcg gctgccgcag atcctgcgcg acttccgcca
    11881 ggacaacccg ctcgtcgacc tcgacctcac catcgatcag agcggcaccc tgcaccgcag
    11941 gctcgagaac gaccgactgg acctgttcgt gggcaagcgc ccgcgcggcg agcaccgcgg
    12001 ccagctcgtc aggcgcgacc gcttggtgtg ggtgggcaac gccgcgaccc gcgtggagtt
    12061 cagccggccg ctcccgctgg tcgtctaccc gccgccgagc ctcagccgcg ttgcgatgca
    12121 gaacgcgctc gagcggacca acaccgcgta ccggagcgcc tgcatctgtc gtggggtgaa
    12181 cggactcatc gccgccgtcg cggcgggcat cggcatctcc gcgctggcgg gcagcctcgt
    12241 cccggtgcag ctgacaacgc tgggcccgca gca
//
LOCUS       DASRSC010000316         5807 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2751248,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000316 DASRSC010000000
VERSION     DASRSC010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2751248"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..645)
                     /locus_tag="VFE39_15590"
     CDS             complement(196..645)
                     /locus_tag="VFE39_15590"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HET6260059.1"
                     /translation="MTEDPTRATARPGSPLHGSVGPADPHADPETNPAMRPVRVPEPE
                     GPTVPTPTTPGAPDTARRPAVTRTRTSGLWTGLLLSAVVLLFLLIFIMQNNAPMGINF
                     LAWSGTLPTGVALLLAAIAGLLLVAIPGGLRILQLRRAAQRSDRHLH"
     gene            complement(783..1247)
                     /locus_tag="VFE39_15595"
     CDS             complement(783..1247)
                     /locus_tag="VFE39_15595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6260060.1"
                     /translation="MTSEAIAATVHRCWAADLEPRTMYEILRLRVEVFVVEQACPYAE
                     LDGRDLEERARHYWLGGSGQPEPVLGCVRLLKEPTGDYRIGRLCTTRTVRGRGLGRRL
                     MEAALAEVGDGGCVLDSQEHLAGFYSRYGFVPTGPTYDWDGVAHVPMRRDGR"
     gene            1369..2745
                     /locus_tag="VFE39_15600"
     CDS             1369..2745
                     /locus_tag="VFE39_15600"
                     /EC_number="1.8.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycothione reductase"
                     /protein_id="HET6260061.1"
                     /translation="MHHHDLVVIGTGSGNSIIDSRFDELDVALVEHGVFGGTCLNVGC
                     IPTKMYVYPADVAEAVRLAARYGIDASIDKVRWSDIRDRVFGRIDPISAGGREYRVER
                     CPNVTVHQGHARFTGHKELVVERTDGSGTDTISGDRIVIAAGGRPAVPEQIASSGVPF
                     HTSDTVMRLDSLPEHILIVGGGYIAAEFAHVFSAFGSRVSIVGRSELLLRSQDETVAE
                     RFTATACERWDVHLGQEVVRASGGGGDVVLELSDGTTLRGDVLLVATGRMPNSDLLDL
                     DRTGVPTHPDGRIVVDDQQRTPVDGIFAAGDVSSPYQLKHVANHESKVVAHNLLHPDA
                     PRRTDHRFVPAAVFTDPQIASVGLTEDECRAAGHDYCVKIQAYGDVAYGWAMEDTGGF
                     CKVLAERGTGRLLGAHVVGVQAATVIQPLVQAMTFGLGAREMATEQYWIHPALPEVVE
                     NALLGLDL"
     gene            2840..3790
                     /locus_tag="VFE39_15605"
     CDS             2840..3790
                     /locus_tag="VFE39_15605"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET6260062.1"
                     /translation="MPMTVAESPSRPAGTARPILDGRRGVAAQIAVYVFVIVPLLALL
                     AAVPFAWGWGLGWTDVVLALVFYVISGLGITVGYHRYFTHGSFKANRPLRIALAVAGS
                     LAMQGSVIDWVADHRRHHAFSDKEGDPHSPWLFGTGPTALARGFWHSHMGWLFERDQT
                     NEQRFTPDLLADRDITRVDRLFVIWSVATLLVPAVLGGLLTWSWWGALTAFFWAGLVR
                     VALLHHVTWSINSICHMIGDQPFAARDHSRNVWPLAVLSFGESWHNLHHADPTCARHG
                     VQRGQVDISARVIRIFEKVGWAHSVRWPTQRRLDKLARTS"
     gene            complement(3816..4610)
                     /locus_tag="VFE39_15610"
     CDS             complement(3816..4610)
                     /locus_tag="VFE39_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HET6260063.1"
                     /translation="MTADRPLTVWQKLVLTFSRRWRGSGFWYGLAIAVIWPFALFGTR
                     IGWRGGEHLPRTGGALLAINHVSFSDPIFNVAFTISHGRMPRFLAKSELWDVPVVRSV
                     LGQGGHIPVYRASTRAGDAYRDAIAAIKRGEIVAFYPEATYTADPDGWPMKGKNGIGR
                     IALVTGAPVIPVGNWGTQDVLPPGSARPRLLSRRKVTVVAGPPVDLSAWLGGPRTRTA
                     LDGATAAIMADVTRLVAEIRGETPPAMPFDPAATGPNGIEQERPAS"
     gene            4852..5553
                     /locus_tag="VFE39_15615"
     CDS             4852..5553
                     /locus_tag="VFE39_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6260064.1"
                     /translation="MPENSLPRIEPVGAPGADAAAVVLVLHGGRARSRESSERRRLAY
                     LRMVPFARTLAAAGPAVYMLRYRVRGWNTPAKDALRDAQWALGEIAQRHPGTPVVLVG
                     HSMGGRAALGAAGAPNVIAVCALAPWLDESDPVDQLAGRIVLIAHGDREHYTDPRESY
                     AYAVRAKRVTDRVARFDVHGAGHFMLSRAGDWRSLVRRFVLGVTGIELEDPEITNALQ
                     QPAPDGLRAALPASR"
BASE COUNT          855 a         2118 c         2000 g          834 t
ORIGIN      
        1 agcacgacat ccgccgcgag ggcgcgatcc tcgaccggca ccggttcgaa cgcgtggcgg
       61 cggagctgcg gacgcgcttc ggctggcgct gacgcccggt accgggcgac tcgcgggcgg
      121 gaacggggcg actcgcgggg caaaacccga cgactcgcgg accggaaccg ggcgactcgc
      181 gggcgggtgg gtcgctcagt gaaggtggcg gtcgctccgc tgcgcggcgc ggcgcagctg
      241 caggatgcgc aggccgcccg ggatggcaac gagcagcagc cccgcgatcg cggcgaggag
      301 cagcgcaaca ccggtgggca gcgtgcccga ccaggcaagg aagttgatcc ccatcggcgc
      361 gttgttctgc atgatgaaga tcaacaagaa gagcaggacc actgcggaca ggagcaagcc
      421 cgtccacaac ccactggtgc gggtgcgcgt gacggccgga cggcgggcgg tgtcgggggc
      481 gcccggcgtg gtcggggtcg gcactgtcgg cccctccggc tccgggacgc gcacgggccg
      541 catcgcaggg ttcgtctccg gatcggcgtg cgggtcggcg gggccgacgc tgccgtgcag
      601 gggtgagccc ggccgtgcgg tcgcccgcgt cgggtcctcc gtcatctgcc tacctcgctc
      661 cgctcacgac gtgcttcctg cgcgttcgaa cgggagtatc ccgcaacggc cggtacggag
      721 cggaacacgg tgcgtcaatg tcgctcggaa ccgctgcaac tcgggaacca cgggaaggtc
      781 gatcagcggc cgtcgcggcg catcggtacg tgcgcgacgc cgtcccagtc gtaggtgggt
      841 ccggtcggga cgaagccgta gcggctgtag aacccggcga gatgctcctg cgagtcgagc
      901 acgcagccgc cgtcgccgac ctccgcgagc gccgcctcca tcagccgcct ccccagcccc
      961 cgtccacgca cagttcgcgt ggtgcacagc cgcccgatgc ggtagtcgcc ggtcggctcc
     1021 ttgagcagcc gcacacaccc gagcaccggc tcgggctgcc cggagccacc gagccagtag
     1081 tgccgggccc gttcctccag gtcgcggccg tcgagctcgg cgtacgggca ggcctgctcc
     1141 acgacgaaca cctcgaccct cagccggagg atctcgtaca tggtgcgggg ctcgagatcg
     1201 gccgcccaac aacggtgcac agtggccgca atcgcctcgc tggtcatgcc gactgcctac
     1261 cagatcggcc gaaccggcgc tgggccggac gtggagctgc cccaccagca gatgtgaggg
     1321 ccgtcggacc gcccggcgtc cacggccgac cgcagctacc gtgatgacgt gcaccaccac
     1381 gatctcgtcg tcatcggcac cgggtccggc aactcgatca tcgacagccg gttcgacgag
     1441 ctcgacgtcg cgctcgtcga gcacggcgtg ttcggtggca cctgcctgaa cgtgggctgc
     1501 atcccgacga agatgtacgt ctatcccgcc gacgtcgcgg aggccgtccg gctcgcggcg
     1561 cgctacggca tcgacgcgag catcgacaag gtccgctgga gcgacatccg cgaccgcgtc
     1621 ttcggccgga tcgacccgat ctccgccggc ggccgggagt accgggtgga acgctgcccc
     1681 aacgtcaccg tccaccaggg gcacgcccgg ttcacggggc acaaggaact cgtcgtggag
     1741 cgcaccgacg gctccggcac ggacaccatc tccggcgacc ggatcgtcat cgccgccgga
     1801 ggacgcccgg ccgttccgga gcagatcgcg tcctccggcg tcccgttcca cacgtccgac
     1861 accgtcatgc ggctcgactc cctgcccgag cacatcctga tcgtcggcgg cggctacatc
     1921 gccgccgagt tcgcgcacgt gttctccgcc ttcggcagcc gcgtctcgat cgtcgggcgc
     1981 agcgagctgc tgctgcgcag tcaggacgag accgtcgccg agcggttcac ggccaccgcc
     2041 tgcgagcgct gggacgtcca cctcggccag gaggtcgtcc gggcgagcgg cggcggtggg
     2101 gacgtggtgc tggagctcag cgacggcacg acgctgcgcg gggacgtgct gctcgtcgcg
     2161 accggccgca tgcccaacag cgacttgctc gacctcgacc ggaccggtgt gccgacccac
     2221 cccgacggtc ggatcgtcgt cgacgaccag cagcgcacgc cggtcgacgg gatcttcgcc
     2281 gcgggcgacg tgagctctcc gtaccagctc aagcacgtgg cgaaccacga gtccaaggtc
     2341 gtggcccaca acctgctgca ccccgacgca ccgcggcgca ccgaccaccg gttcgtgcct
     2401 gccgcggtct tcaccgatcc gcagatcgcc tccgtcggcc tcacggagga cgagtgccgt
     2461 gcggccggcc acgactactg cgtcaagatc caggcctacg gcgacgtcgc gtacggctgg
     2521 gcgatggagg acaccggcgg cttctgcaag gtgctcgccg aacgaggcac cggcaggctc
     2581 ctcggcgcgc acgtggtggg tgtgcaggcc gccacggtga tccagccact ggtccaggcg
     2641 atgacgttcg gcctcggcgc ccgcgagatg gccaccgagc agtactggat ccatccagcc
     2701 ctgccggagg tcgtggagaa tgcgctgctc ggactcgacc tgtaggacgt cacacacgtt
     2761 ttggggtgcc accgggcagg gaatgcctga tagacaacgg atcagtaagg tgacccttcc
     2821 tgcgagagcc acgaggtgca tgcccatgac cgttgccgaa agtccgagcc gaccggccgg
     2881 aacggccaga ccgatcctgg acggcaggcg cggcgtcgcg gcgcagatcg ccgtctacgt
     2941 gttcgtgatc gtcccgctgc tggcgctgct cgctgccgtc ccgttcgcct ggggctgggg
     3001 cctgggctgg acggacgtcg tgctcgccct ggtcttctac gtgatcagcg gcctcggcat
     3061 caccgtcggc taccaccggt atttcaccca cggctcgttc aaggcgaacc ggccgctgcg
     3121 catcgcgttg gcggtcgccg gcagcctcgc gatgcaggga tcggtgatcg actgggtggc
     3181 cgaccatcgc cgtcaccacg cgttctccga caaggagggt gacccgcact cgccgtggct
     3241 gttcggaacc gggcctacag cgctggcccg cggcttctgg cactcgcaca tgggctggtt
     3301 gttcgagcgc gaccagacca acgagcaacg cttcacgccc gacctgctcg ccgatcgcga
     3361 catcacgcgc gtcgaccgct tgttcgtcat ctggtcggtg gccacactgc tcgttccggc
     3421 cgtgctcggt ggcctgctga cgtggtcgtg gtggggtgca ctcaccgcgt tcttctgggc
     3481 cggcctggtg cgcgtcgcgc tgctgcacca cgtcacctgg tcgatcaact cgatctgcca
     3541 catgatcggc gaccaaccct tcgcagcccg cgaccactcg cgcaacgtct ggccactcgc
     3601 cgtgctcagc ttcggcgagt cgtggcacaa cctgcaccac gcggacccca cctgcgcccg
     3661 gcacggcgtg cagcgcggcc aggtcgacat ctcggcccgc gtgatccgga tcttcgagaa
     3721 ggtcggctgg gcccactcgg tgcgttggcc cacgcagcgg cggctggaca agctggcccg
     3781 caccagctga accggcggtg gccccgcgga cggcgtcagc tcgcggggcg ctcctgctcg
     3841 atgccgttcg gcccggtcgc ggccgggtcg aacggcatgg cgggtggcgt ctctccccgg
     3901 atctcggcca ccagccgcgt gacgtccgcc atgatcgcgg cggtcgcgcc gtcgagggcg
     3961 gtgcgggtac gagggccacc cagccacgcg gagaggtcga cgggtggtcc ggccacgacc
     4021 gtcaccttcc ggcgcgacag caaccggggg cgggcactgc ccggaggcag aacgtcctgc
     4081 gtgccccaat ttccgaccgg gatcaccggt gccccggtga ccagtgcgat ccggccgatc
     4141 ccgttcttgc ccttcatcgg ccagccgtcc gggtcggcgg tgtaggtggc ctccggatag
     4201 aacgccacga tctcgccacg cttgatcgcg gcgatggcgt cgcggtaggc gtcgccggcg
     4261 cgggtggagg cgcggtacac cgggatgtgc ccgccctggc ccagtaccga gcgcacgacc
     4321 ggcacgtccc agagctccga tttcgcgagg aagcggggca tccggccgtg cgagatcgtg
     4381 aaggcgacgt tgaagatcgg gtccgagaac gacacgtggt tgatcgccag caacgcaccg
     4441 ccggtgcggg gcaggtgttc accgccccgc cagccgatcc gggtgccgaa cagcgcgaat
     4501 ggccagatca ccgcgatcgc gagcccgtac cagaacccag acccgcgcca gcgccgagag
     4561 aacgtcagta cgagcttctg ccacaccgtg agcggccggt ccgccgtcat acgtcctcct
     4621 cgcgcgctgt ccggcgtccg ttcccgcacg ccgcctctcg atcatctcag gcgcccaacg
     4681 gtggtcccac cggcgcagca cccgctccag ggtgcccgtc ccccatgacc ggagggttcg
     4741 cgacccggat gtgatctcgg tcccagcagt gatccggcag gcccagatca gcggttacca
     4801 ggacgcagag tgcatatccg tcccgctgcc gaccgcccgc gaggtactcc cgtgccagag
     4861 aactccctcc cccgcatcga gccagtgggt gcgcccggcg cagatgccgc cgcggtggtg
     4921 ctcgtcctgc acggcgggcg cgcccgcagc cgggagagca gcgaacggag gcggctggcg
     4981 tacctgcgga tggtgccgtt cgcgcggacg ctcgccgccg ccggccccgc cgtctacatg
     5041 ctgcgctacc gcgtccgcgg atggaacaca ccggcgaagg acgccctgcg cgacgcgcag
     5101 tgggccctcg gcgagatcgc gcagcgacac cctggcaccc ctgtcgtgct ggtcgggcac
     5161 tcgatgggtg gccgggcagc gctgggcgcg gcaggtgccc ccaacgtcat cgccgtgtgc
     5221 gcgctcgcgc cgtggctgga cgaatccgat ccggtggacc agcttgccgg ccgcatcgtc
     5281 ctcatcgccc acggcgaccg cgagcactac acggacccgc gcgagtccta cgcctacgcc
     5341 gtgcgggcca agcgcgtgac cgaccgcgtc gcccggttcg acgtgcacgg cgccggtcat
     5401 ttcatgctca gccgcgcggg cgactggcgg tcgcttgtcc gtcgcttcgt gctcggggtc
     5461 accggaatcg aactcgagga ccccgagatc acgaacgctc tgcagcagcc cgcaccggac
     5521 ggcctgcgcg cggcgcttcc cgcctctcga tgactcgacc acccggcccc ggcgcccgcc
     5581 ggggccgggt gatggactgc gcgctccggc tacacacgcc acccgaacta catcggtgac
     5641 accgcgcgtc tcggccgcac ccgaggccgt gttcgcgctg ctcgcgaacc cacaccgaca
     5701 ccacgagttc gacggttcgg gcaccgtccg gagggcggta tccggtccgc agcagctcac
     5761 tctcggcgcc cggttcggga tggacatgaa gatgcggctc ccgtatc
//
LOCUS       DASRSC010000317         4626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1115445,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000317 DASRSC010000000
VERSION     DASRSC010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4626
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115445"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..886
                     /locus_tag="VFE39_15620"
     CDS             <1..886
                     /locus_tag="VFE39_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HET6260065.1"
                     /translation="PSEAVKVALTLWGAHVLVARRAVMHRWKYALSPIVPVSVVLLTL
                     LVLQPDLGMTVSLGIVLIALLFFAGAPMRLMVALTVGALGGAIVLALSASYRLSRISA
                     FLSPESVDPLKAGFQSNQALYSLADGGLFGVGLGQGRAKWSYLPNAHNDFIFAIIGEE
                     LGFLGAFAVLALFATLAYTGLRIAARNTDPWLKLVVATSTTWLVAQAAINISYVVGLL
                     PVTGLQLPLISSGGTSLVVTMFVFGILANAARHEPEAIAALKQHGQGRVARLLRLPLP
                     LPYRAPAARRPTEWGRVR"
     gene            883..2058
                     /locus_tag="VFE39_15625"
     CDS             883..2058
                     /locus_tag="VFE39_15625"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET6260066.1"
                     /translation="MSSGPAGRRAPSVVVAGGGSAGHIEPALALADAVRRLRPDARIT
                     ALGTERGLDTTLIPARGYPLELIPPVPLPRKPSADLLRLPGRVRDAVTRVREVLDAVQ
                     ADVVAGFGGYVALPAYLGARGRVPIVVHEANARAGLANKVGARCAAFVAVAVPGTGLP
                     GEQVVGMPLRRSITGLDRMALRAQARHWFGLPPHGPVLLVFGGSQGARTLNEAVAAAL
                     PGLLRAGIAVLHAHGRGGSAAPPARGYVATPYIERMDLAYAAADAVLGRCGMTTVAEV
                     TAVGLPAVYVPLPHGNGEQALNAAPVVALGGGVLVDDAELTGERAMAELIPLLTNPGR
                     LTAMGRAARSTGHADAADRLARLVLEIADGYGPGTDRGHPGTESHGLRKGPDPWAGA"
     gene            2058..3452
                     /gene="murC"
                     /locus_tag="VFE39_15630"
     CDS             2058..3452
                     /gene="murC"
                     /locus_tag="VFE39_15630"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006239850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET6260067.1"
                     /translation="MAVELGGRVHLIGIGGAGMSGIARILLARGATVSGSDAKDSRAV
                     LALRALGARVEVGHDPGHLPHAPATVVVSSAIRATNPELAAARERGLDVVHRAQALAA
                     LTEGRRLAAVTGTAGKTSTTSMLTVALQHCGLDPSFAIGGDLAASGSGAHEGSGDIFV
                     AEADESDASFLAFSPMVAVVTNVEADHLDHYGTAEAYVAAFETFLDRIAPGGALVACS
                     DDSGAEALARVAEGRGIHVRRYGRGGCDAQLLDFRPDGTGARVVLRHDGVQHLLRMAV
                     PGEHMALNALAALLAGVELGAGVDGLLAGLAAFDGVRRRFEFRGRAAGVAVYDDYAHH
                     PSKVAAQLRAARDVVGDRARVVVAFQPHLYSRTREFAGAFGAALGLADEVVVLEVYGA
                     REDPEPGVSGALVADAVTLPPDRVHYVPRWSDVPSVVAGVARPGDLVITMGAGDVTVL
                     APEILLELERRERA"
     gene            3664..4353
                     /locus_tag="VFE39_15635"
     CDS             3664..4353
                     /locus_tag="VFE39_15635"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HET6260068.1"
                     /translation="MLIALLLLVGLGLTGRVLLYDMGLADVEDVQVTSAGAGGTTSGS
                     PIVPVDAVLAAAAVTRGAPLAAVDTGAVAGRVAQVPGVESVRVSRAWLHTVTIEITER
                     VAVATVPTLQGPALVDRLGVVYPGPASPGLPRFTFRPAGPDDPATRAAVSVLTALPEA
                     LRSQVVTVDATDTAPDVPGQVTLGLTENRQVQWGSPDRSGEKAAVLVPLLTQRGQVYD
                     VTSPDLPTIKR"
BASE COUNT          549 a         1680 c         1734 g          663 t
ORIGIN      
        1 gccgtcggag gcggtcaagg tcgccctgac gctgtggggt gcgcacgtgc tcgtcgcccg
       61 gcgcgcggtg atgcatcgct ggaagtacgc gctgtcgccg atcgtgccgg tctccgtggt
      121 gctgctcacc ctgctcgtgc tgcagccaga cctcggcatg acggtctcgc tgggcatcgt
      181 gctcatcgcg ctgctgttct tcgcaggcgc cccgatgcgg ctgatggtcg cgctgaccgt
      241 gggtgcgctt ggcggagcca tcgtgctcgc gctcagcgcg agctaccggc tgagccggat
      301 cagcgcgttc ctctcgcccg agagcgtcga cccgctcaag gccggtttcc agtccaacca
      361 ggcgctctac tcgctcgccg acggcgggct gttcggcgtc gggctcgggc agggccgggc
      421 gaagtggagc tacctgccca acgcccacaa cgacttcatc ttcgcgatca tcggcgagga
      481 gctgggattc ctgggtgcgt tcgccgtgct cgcgctgttc gccacattgg cctacacggg
      541 cctgcgcatc gcggcccgca acaccgaccc gtggctcaag ctcgtcgtgg ccacctccac
      601 gacctggctg gtggcgcagg ccgccatcaa catctcctac gtcgtggggc tgctgccggt
      661 gaccgggctg cagctcccgc tgatctcctc gggaggtacc tcgctcgtgg tcaccatgtt
      721 cgtcttcggg atcctcgcca acgcggcgcg tcacgagccg gaggccatcg cagcgctcaa
      781 gcagcacggc cagggccgcg tcgcgcggct gctgcggctg ccgttgccgt tgccctaccg
      841 agcccctgcc gcccgccggc ccacagagtg gggacgggtc cgatgagcag tgggcctgcg
      901 gggcgccgcg ctccctcggt cgtcgtcgcg ggcgggggga gtgccgggca catcgaaccc
      961 gcgctcgccc tcgcggacgc cgtgcgcagg ctgcggcccg acgccaggat caccgccctc
     1021 ggcaccgaac gcggcctcga tacgacgctc atcccggccc gcggctaccc actggagctg
     1081 atcccgccgg tgccgctgcc gcgcaagccg agcgctgacc tgctgcggct gcccggccgg
     1141 gtgcgcgacg cggtcacgcg ggtccgcgaa gtgctcgacg ccgtgcaggc cgacgtcgtg
     1201 gcgggcttcg ggggatatgt ggcgctgccg gcctacctgg gggcccgcgg ccgggtgccg
     1261 atcgtcgtcc acgaggcgaa cgcgcgggcg gggctggcca acaaggtcgg tgcgcggtgc
     1321 gcggctttcg tggccgtcgc ggttcccggc accgggctgc cgggcgagca ggtcgtcggg
     1381 atgccgctgc ggcggtcgat caccgggctg gatcggatgg ccctgcgcgc gcaggccagg
     1441 cattggttcg gccttccccc gcacgggccg gtacttctgg tgttcggcgg ctcgcaaggt
     1501 gcccggacgc tgaacgaggc cgtcgccgcc gcactgcccg gcctgctgcg ggccgggatc
     1561 gcggtcctgc acgcgcacgg gaggggtggc agcgcggcac cacccgcccg tggctacgtc
     1621 gcgacgccct acatcgagcg gatggacctg gcctacgccg cggccgacgc cgtgctcgga
     1681 cggtgcggca tgacgacggt cgccgaggtc accgccgtcg ggctcccggc cgtctacgtg
     1741 ccgctgccgc acggcaacgg cgagcaggcg ctcaacgcgg cgcccgtcgt cgcactgggc
     1801 gggggagtgc tcgtggacga cgccgaactc accggggagc gggccatggc cgagctgatc
     1861 ccgttgctga ccaaccccgg gcgtctgacg gcgatgggcc gggcggcccg ctccaccggc
     1921 cacgccgacg cagcggaccg cctcgctcgc ctggttctcg agatcgccga cgggtacggt
     1981 ccgggcacgg atcgtggcca tccgggcacc gagagccacg gcctgcgcaa aggacctgat
     2041 ccgtgggcgg gggcgtgatg gctgtcgagc tgggtgggcg cgtccacctc atcgggatcg
     2101 gtggcgccgg gatgagcggc atcgcccgga tcctgctcgc caggggggcc accgtctcgg
     2161 gttcggacgc gaaggactcg cgtgcggtgc ttgccctgcg cgcgctcggt gcgcgcgtcg
     2221 aggtcggcca cgaccccggg cacttgcccc acgcgcccgc caccgtcgtc gtctcgagcg
     2281 cgatccgtgc caccaacccc gagcttgccg ccgcgcgcga gcggggtctc gacgtggtgc
     2341 accgcgccca ggccctcgcc gccctgacgg agggcaggcg cctcgcggcc gtcaccggca
     2401 cagcgggcaa gacgtccacc acctcgatgc tcaccgtcgc gctgcagcac tgcgggctgg
     2461 atccgtcgtt cgcgatcggc ggtgacctcg ccgcgtccgg ctcgggcgcc cacgagggca
     2521 gcggcgacat cttcgtggcc gaggccgacg agagtgacgc gtcgttcctc gcgttctccc
     2581 cgatggttgc ggtggtcacc aacgtcgagg ccgaccatct cgaccactac ggcaccgcag
     2641 aggcctacgt cgcggcgttc gagacgttcc tcgaccggat cgcgcccggc ggcgccctcg
     2701 tggcctgctc cgacgactcc ggcgcggagg cgctggcccg ggtggcggag ggccgcggta
     2761 tccacgtgcg ccggtacgga cgtgggggct gcgacgcgca gctgctggac ttccgtccgg
     2821 acggcaccgg ggcgcgggtc gtgctgcgcc acgacggcgt ccagcacctg ctccggatgg
     2881 ccgtacccgg cgagcacatg gcgctcaacg cgcttgccgc gctgctcgcg ggcgtcgagc
     2941 tgggcgcggg cgtcgacggc ctgctggcgg ggctcgccgc gttcgacggc gtgcgtcgcc
     3001 ggttcgaatt ccgcggccgg gcggccggcg tcgcggtcta cgacgactac gcccaccacc
     3061 cgagcaaggt cgcggcccag ctgcgggctg ctcgggacgt ggtgggggac cgggccaggg
     3121 tggtggtggc cttccagccg cacctctact cccgtacccg tgagttcgcg ggcgcattcg
     3181 gggcggcgct cgggctcgcc gacgaggtgg tcgtgctgga ggtctacggc gcgcgcgagg
     3241 accccgagcc cggggtcagc ggcgcgctcg tggccgatgc cgtgacgctg ccgcccgacc
     3301 gcgtgcacta cgtgccgcgc tggtcggacg tgccgtccgt cgtggccggc gtggcgcggc
     3361 ccggcgacct cgtgatcacg atgggcgccg gcgacgtcac cgtgctggca ccggagatcc
     3421 tgctcgagct ggagcggcgg gagcgggcat gagcggacat gccatcggca ggcgatcgag
     3481 ctcgaggagc gtcggaaagg cgggcgagtg agcggttcga caaccccccg ccccgccgct
     3541 tcccgcccgg cggccgcggg ttcgaccgtg gcccggccca cccggcgtcc gactggtcgg
     3601 ccggaccgcc ccccgccgcg accggtcccg gcgtcccgtt accggcgccg caggctcgct
     3661 gcggtgctga tcgcgctgct gctgctcgtc ggactgggcc tgaccgggcg ggtgctgctc
     3721 tatgacatgg ggctggccga tgtcgaggac gtgcaggtca cctcggcggg tgcaggtgga
     3781 acgacgagcg gttcccccat cgtgccggtc gacgccgtgc tcgccgcggc ggcggtgacc
     3841 aggggagccc cgctggccgc cgtcgacacc ggcgcggtcg ccggacgggt ggcgcaggta
     3901 cccggcgtgg agtcggtccg ggtctcgcgg gcctggctgc acacggtcac catcgagatc
     3961 accgagcggg tcgcggtcgc gacggtgccg accctgcaag gccccgcgct cgtggaccgc
     4021 ctcggcgtgg tctaccccgg cccggcctcg cccgggctgc cgcggttcac cttccggccc
     4081 gccgggccgg acgaccccgc cacgcgcgca gcggtgagcg tgctcacggc gctgcccgaa
     4141 gcgctccggt cgcaggttgt gaccgtggac gccacggaca cggcgccgga cgtgccgggg
     4201 caggtcactc tcgggctgac cgagaacagg caggtccagt ggggttcccc ggaccgctcg
     4261 ggggaaaagg cggcggtgct cgtgccgttg ctcacccagc gcggtcaggt ctacgacgtg
     4321 acgagtccgg acctgcccac gatcaagcgg tgagcagcga gcatacgatt tcgggcattc
     4381 cgacgccgtg acgccgagcg atcgcagcgc cggagcaggt gaggtcaaga tcgacccggt
     4441 cggggggttg cctgcacgcg gcgcgcctgc tggaccggga ccctaacgcg acctagcgtt
     4501 cgaacatgaa ggcccggacc cccggggtcc gacccgaagc tggactggaa ggtgcgccat
     4561 gacgcccccg cacaactacc tggccgtcat caaggtcgtc ggcatcggtg gcggcggcgt
     4621 gaacgc
//
LOCUS       DASRSC010000318         4059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5538973,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000318 DASRSC010000000
VERSION     DASRSC010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5538973"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..556
                     /locus_tag="VFE39_15640"
     CDS             110..556
                     /locus_tag="VFE39_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6260069.1"
                     /translation="MTVADDAAELRVRGWRTLAALHGRLNEALEKALQSGFGLSVVEY
                     TVLDTLGRQDGWHMRMQQLAGAATLSHSATTRLVTRLETRRLLSRYLCDDDRRGIYST
                     LTAQGKELLEQARPVHDRTLKESLDAARAVPELAPLVDALHALPHP"
     gene            577..1812
                     /locus_tag="VFE39_15645"
     CDS             577..1812
                     /locus_tag="VFE39_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6260070.1"
                     /translation="MTQDRPATAPTRDRPNPQYAHLGEALGTDFFSVRDQFTDEQWNR
                     FIAVRKFVDDEVLPDIGEYWEKAELPWHLFRRLPELGIVGEDIDGYGAAAMSPLACGL
                     VHMELHRGDGSLGTFLAVHAGLAMQSIAMCGSEEQKQRWLPSMSTMDSVGAFALTEPQ
                     HGSDSVALETTARRDGDRWVLNGAKRWIGNATIADLVVVWARDTDSGDVLGFVVETPA
                     DGYTASVITGKVSLRSVWQADITLSGVRVPDENRLTNARTFRDCGRVLANTRGICAWM
                     ALGHATAGYDAAMRYAMARQQFGRPLASFQIVQQRLVGMLADLTGMQLYCMQLGRLAD
                     AGRLSPTVAGLAKMHNTRTARKILSEARDLLGGNGILLEHHVVRHWADIEAIHTFEGT
                     ETMQTLIVGRDITGIGAFT"
     gene            complement(1849..2229)
                     /locus_tag="VFE39_15650"
     CDS             complement(1849..2229)
                     /locus_tag="VFE39_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET6260071.1"
                     /translation="MSRSVHDLLAAARSRLDRPDPHRAAAMVERGAILVDTRPGWQRE
                     QEGELPGALVIERNHLEWRLDPSSDARIPEATDHDVTWIVFCSEGYSSSLAAASLQDI
                     GLHNATDLDGGYQAWTKAFGPVRG"
     gene            complement(2404..2892)
                     /locus_tag="VFE39_15655"
     CDS             complement(2404..2892)
                     /locus_tag="VFE39_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine dioxygenase family protein"
                     /protein_id="HET6260072.1"
                     /translation="MLLHAPTPLVLADLTALTREVADEVHAGRHAITIDPERRWYRLL
                     RSDSYVDVWLISWATEQTAELHDHAGSIGALTVVSGSLVEQRWVGDGLRSRRLRAGRS
                     VGFPLGHVHDVGNPEPTPAVSVHAYSPPLTAMSYYAVEPDRRLRRTRSVLVESGSSEG
                     VG"
     gene            3129..3836
                     /locus_tag="VFE39_15660"
     CDS             3129..3836
                     /locus_tag="VFE39_15660"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HET6260073.1"
                     /translation="MATLLDDGSWDVQGRRLQFPVRITAATAAFATYLVRTARAGSLV
                     SGTGLELVSVAGRTPLFLGFVTYRAGDLDSYHEVAVAFLARHRGRVGPYIHQLPVTQT
                     FTMEAGRALWGLPKWLARAELDITGRDATCHLTDDADRHVLTAALRTLPWRIPLTIPG
                     ALTALAPRGDTVLISRVRAKVGGIRFGVRGATAVLGSGHPMADELRAVGLPRRPVATL
                     TAEHLEFDMAAAVAVPR"
     gene            complement(3895..>4059)
                     /locus_tag="VFE39_15665"
     CDS             complement(3895..>4059)
                     /locus_tag="VFE39_15665"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6260074.1"
                     /translation="DELTDPAGSRLLESTSGSPDQTVIWYEGLWHEIYNEPEREGPLS
                     DLREWLAAHR"
BASE COUNT          609 a         1468 c         1391 g          591 t
ORIGIN      
        1 cctactcggc cggttgttac ctgcgcatct aacaggcgta agcaacagtt gcacacgcgc
       61 tctatcggcg gcaacccggt atgctcctcc ggagaagcgg aggtgaccga tgaccgtcgc
      121 ggacgatgcc gccgagctgc gggtccgggg ctggcgcacg ttggccgcgt tgcacggacg
      181 cctgaacgag gcgctggaga aggcgctgca gtccgggttc gggttgtctg tcgtcgagta
      241 cacggtgctc gacactcttg gccggcagga cggctggcac atgcgcatgc agcagctcgc
      301 cggcgccgcg acgctctcgc acagcgccac gacgcgcctc gtcacgcgtc tggagacccg
      361 ccgtctgctg agccgctatc tctgcgacga cgaccggcgc gggatctaca gcacgctcac
      421 cgcgcagggc aaggagctgc tggagcaggc acgtccggtg cacgacagga cgctgaagga
      481 gagcctcgac gccgcgcgcg ccgtacccga gctcgcccca ctcgtcgacg ccctgcacgc
      541 actgccgcac ccctgacgac gtgccaatat cggccgatga cccaggaccg gccggccacc
      601 gcccccacca gggatcgtcc gaacccccag tacgcccacc tcggcgaggc gctgggcacc
      661 gacttcttct ccgtgcgtga tcagttcact gacgagcagt ggaaccgatt catcgccgtc
      721 cggaagttcg tcgacgacga ggtgctgccc gacatcggtg agtactggga gaaggccgag
      781 ctcccctggc acctgttccg caggctgccc gagctgggga tcgtcggtga ggacatcgac
      841 ggctacggcg cggccgcgat gagcccgctg gcctgcgggc tggtgcacat ggagctgcac
      901 cgcggcgacg gctcgctcgg cacgttcctc gccgtgcacg ccgggctcgc catgcagtcg
      961 atcgcgatgt gcggctcgga ggagcagaag cagcgctggc tgccgtccat gtccacgatg
     1021 gacagcgtcg gggcgttcgc actcaccgag ccccagcacg gttcggactc cgtcgccctc
     1081 gagaccacgg cccgccgcga cggcgaccgc tgggtactca acggcgccaa gcgctggatc
     1141 ggcaacgcca cgatcgccga cctcgtggtc gtgtgggccc gcgacaccga ctccggcgac
     1201 gtgctcggct tcgtcgtcga gacccccgcc gacggctaca ccgcgagcgt gatcaccggc
     1261 aaggtgtcgt tgcggtccgt gtggcaggcc gacatcacgc tgtccggggt gcgggtgccg
     1321 gacgagaacc ggctcacgaa cgcccggacg ttccgggact gcgggcgggt gctcgccaac
     1381 acccgcggga tctgcgcgtg gatggctctc gggcacgcga cggcgggcta cgacgcggcg
     1441 atgcgctacg ccatggcccg gcagcagttc ggcaggccgc tggcgtcgtt ccagatcgtg
     1501 cagcagcggc tcgtgggcat gctcgcggac ctcaccggca tgcagctcta ctgcatgcag
     1561 ctgggtcggc tggccgacgc cggacggctg tcgcccaccg tcgccggact ggcgaagatg
     1621 cacaacaccc gcaccgcacg gaagatcctc tccgaggccc gcgacctgct ggggggcaac
     1681 ggcatcctgc tcgagcacca cgtggtgcgg cactgggccg acatcgaggc catccacacg
     1741 ttcgagggca ccgagacgat gcagaccctc atcgtcgggc gcgacatcac cgggataggc
     1801 gccttcacct gacggcagtc gtgcgcggat acgagtgctc cggcactcct atccgcgcac
     1861 ggggccgaag gccttcgtcc aggcctgata cccgccgtca agatcggtgg cgttgtgcaa
     1921 gccgatgtcc tgcagcgacg ctgcggcgag gctggagctg tagccctccg agcagaagac
     1981 gatccaggtc acgtcgtggt cggtggcctc gggaatgcgc gcgtcggagg acgggtcgag
     2041 gcgccactcc aggtggttgc gctcgatcac cagggcaccc ggcagctcgc cctcctgctc
     2101 ccgctgccac cccggccggg tgtcgacgag gatcgcgcct cgctccacca tggcggcggc
     2161 gcggtgtggg tccgggcggt ccagccgcga gcgggcggcg gccagcaggt cgtgcacgct
     2221 ccgactcatc gcactccctc gctgcgctcg gtcggcgctt cgcgaaggcg cagtaacacc
     2281 ggttcgctcg caagctcgct cactcgcact ccctcgccgc gctcggtcag cgcttcgcga
     2341 aggcgcagta acaccggttc gctcgcaagc tcgctcacta gcacttcctc gctcgcaact
     2401 cgctcatccg actccctccg acgagcccga ctcgaccaga acgctgcggg tgcgacgcag
     2461 ccggcggtcc ggctccaccg cgtagtacga catggcggtc aacggcgggg agtaggcgtg
     2521 cacgctcaca gcgggcgtcg gttcggggtt gccgacgtcg tgcacgtgcc cgagcgggaa
     2581 gcccacgctg cgcccggccc gcaaccgtcg cgaccgcagc ccgtcgccca cccagcgctg
     2641 ctcgacgagg ctgccggaca cgacggtgag ggctccgatc gagccggcat ggtcgtgcag
     2701 ctccgccgtc tgctcggtgg cccagctgat cagccacacg tcgacgtagc tgtcgctgcg
     2761 cagcagccgg taccagcggc gttcgggatc gatcgtgatg gcgtggcggc cggcgtgcac
     2821 ttcgtccgcg acctcacgcg tcagagcagt gaggtcggcg aggaccagcg gcgtgggagc
     2881 gtgcagcagc acggacgtac tccagtgacg acggcggacg agcgggcgaa aatgtcagct
     2941 cgtgcgacag cactggtcga agtggtcggc ccgacggcaa ccccagatct ctccgaggac
     3001 ggcggcaggc gacacgacaa catctgagca caccggacgt ctcgcatgcc acctcgtccc
     3061 gaggcgcggg aaggagcggc gccgcgccgg tcaaatgtcc gacagagctg tgcgtcagac
     3121 tgcggtgcgt ggcgactctc ctcgatgacg gcagctggga tgtccagggg cgcaggctcc
     3181 agttcccggt gcggatcacc gccgccaccg ccgcgttcgc gacctacctc gtccgcaccg
     3241 cccgagcggg cagcctggtc tcgggcacgg ggctggagct cgtgtccgtg gccggccgga
     3301 cgccgttgtt cctggggttc gtcacttacc gggccggcga cctcgacagc tatcacgagg
     3361 tcgccgtcgc atttctggcc cgccatcgcg gacgagtcgg cccgtacatc caccagctcc
     3421 cggtgacgca gacgttcacg atggaggcgg gccgggcgct ctggggactc ccgaaatggc
     3481 tggcccgcgc cgagctcgac atcacgggcc gcgacgccac ctgccacctc actgacgacg
     3541 ccgaccggca cgtgctcacc gccgcgttac gcaccctgcc gtggcggatc cccctgacca
     3601 tccccggtgc gctcaccgcc ctggcaccgc gtggggacac cgttctgatc agcagagtgc
     3661 gtgccaaggt cggcggcatc cggttcggcg tccgcggcgc caccgccgtg ctgggctccg
     3721 gccacccgat ggccgacgag ctgcgagccg tcggcctccc ccggcgcccg gtggcgacgc
     3781 tgaccgcgga acacctcgag ttcgacatgg ccgccgcggt cgcggtcccc cgctgatccc
     3841 cgcggaatcc aggtctccac cccggcgagg gcggatcagc gggtcccgcg gcgatcagcg
     3901 gtgcgccgcg agccattccc tcaggtccga cagcgggcct tcgcgttccg gctcgttgta
     3961 gatctcgtgc cagaggccct cgtaccagat caccgtctgg tccggcgagc cgctggtgga
     4021 ctccagcagc cgggagccgg ccgggtcggt gagctcgtc
//
LOCUS       DASRSC010000319         7978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3572937,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000319 DASRSC010000000
VERSION     DASRSC010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7978
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3572937"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="VFE39_15670"
     CDS             complement(<1..1086)
                     /locus_tag="VFE39_15670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6260075.1"
                     /translation="MTGPLLHLATPAEWRAALAVCAIAPPSLAEVGFVHLSTPEQVAL
                     PAGRLFPGRSDLVLLVLDPDRIGVPIRFEPGLPTDPPSMRFPHAYGPVPAAAVLAVLP
                     YRPRGDGGFDAPVIERTDPGWRHAVLEPSLLRRTATAEVPVAGGIAVRTASVPASPEH
                     NQLLVSGRVDATQVAADADRALNGAPHRWALLTGAHLGATAAGLAARGWEVQELVGMA
                     APAGGEPVARVEQVDVEALRPLVEADWRSAVPGIGAAELAQIVAGQRLEEAVLDVRYL
                     AVPSGGELVASAILKIDGGTALLDAVTTEPEHRRRGHGDALLRTAMALAAEAGCDLVV
                     LDAAAGEWPEQWYARRGFVEVTRSWSAR"
     gene            1198..1299
                     /gene="mftA"
                     /locus_tag="VFE39_15675"
     CDS             1198..1299
                     /gene="mftA"
                     /locus_tag="VFE39_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677709.1"
                     /note="Mycofactocin is a small molecule electron carrier
                     derived from the final two amino acids, Val-Tyr, of MftA,
                     the mycofactocin precursor. It plays a role in redox
                     homeostasis and the metabolism of alcohols and aldehydes
                     in Actinobacteria, including Mycobacterium tuberculosis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin precursor MftA"
                     /protein_id="HET6260076.1"
                     /translation="MSPIEQSEPTTEEPLVEETLVEEVSIDGMCGVY"
     gene            1319..1606
                     /gene="mftB"
                     /locus_tag="VFE39_15680"
     CDS             1319..1606
                     /gene="mftB"
                     /locus_tag="VFE39_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332343.1"
                     /note="MftB, a small protein, is a peptide chaperone that
                     assists the radical SAM enzyme MftC in performing two
                     modifications to the C-terminal Val-Tyr dipeptide of the
                     mycofactocin precursor peptide, MftA. MftB's role is
                     analogous to the role of PqqD in the biosynthesis of PQQ,
                     a cofactor that derives entirely from a Tyr and a Glu in
                     the precursor PqqA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis chaperone MftB"
                     /protein_id="HET6260077.1"
                     /translation="MSAQADAGFDPDRAYRCSPRVALRPEPFGALVYHFGTRRLSFLK
                     TPQLVAVVSGLEQHDSVHAAIDAAAVDEAQRPAYLRALAGLATNGTIEERQ"
     gene            1603..2853
                     /gene="mftC"
                     /locus_tag="VFE39_15685"
     CDS             1603..2853
                     /gene="mftC"
                     /locus_tag="VFE39_15685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677707.1"
                     /note="MftC is a radical SAM/SPASM enzyme that catalyzes
                     the first two steps in biosynthesis of the electron
                     carrier mycofactocin from the terminal Val-Tyr dipeptide
                     of the precursor peptide MftA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA];
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin radical SAM maturase"
                     /protein_id="HET6260078.1"
                     /translation="MKLVDHFQYGLNSPICLTWELTYACNLACVHCLSSSGRRDPREL
                     TTAEAKGVIDELQRMQVFYVNIGGGEPTVRPDFWELLDYAVGHDVGVKFSTNGIKLDE
                     KRAQQLAATDYVDVQISLDGATAEINDHVRGPGSYATAIRALENLAAAGFGQPKISVV
                     MTRQNVDQLDEFATIADRFGAQLRITRLRPSGRGADVWDELHPTAAQQRQLYDWLVAH
                     GDNVLTGDSFFHLSAYGEALPGLNLCGAGRVVCLIDPVGDVYACPFAIHENFLAGNVR
                     SPGGFQQVWRQSELFTQLRQPQTGGACRSCGFYDSCQGGCMAAKFFTGLPLDGPDPEC
                     VRGFGEQALAAREADTLVPRSDVDHSRSARRNSRGPVPVQIGMPGRADGQQVRPDRAC
                     ETSPLAGIPEDASDSRLRVPVAGG"
     gene            2956..5130
                     /locus_tag="VFE39_15690"
     CDS             2956..5130
                     /locus_tag="VFE39_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6260079.1"
                     /translation="MRSLVRPVTLGGRVAPSRVMFGPHETNLARFREFSDRHVAYYAR
                     RAAGGAGVIVTEVASVHDSDWPYERAPRAADATGGWTEVANACRPYGPLVLAGLGHAG
                     AQGSSAYGQQALWGPSGVADVVSREVPMELEQPEIDELVTGFSAAAELAAMAGLDGVE
                     INAGQHSILRQFLSGLTNRRADGYGRDRARLLREVLAAARSGLDRDGRGDRVLGLRLC
                     ADELAPWGGITPEQAIATACTVAGSIGYLVVVRGSGMSVFATRPDLHVPPGFNRLLCA
                     TVRAGLGEAVPVVLQGSVVDPGFAQAALDDGVADLVEMTRSQIAEPDLVAHVRAGTLE
                     RIRPCTLSNQHSGVRDPRNPIVSDEAEPRAGHETVDPPVSSPASRPFPARESPTICPR
                     AARIPPASRPFSARESPAIGARVARYPPPSRPVARAPVSEEAPTGESRTNAVRVRRSV
                     LVVGGGPAGMEAARVLTLRGHRVRLAERSGRLGGALLLAAAVAGRERMGVLVPWWERE
                     LARLGVEVQTGVEVGVPELDGAQRDGTAVLLATGSRPAPRPYRSDGPVLDAAEFEAAV
                     LAAGSTEAVLAGLPGGTVVVHDPIGDWTGVGIAEQVVATGRPTAIVTRDPVAGAQLGR
                     TGDLVPANTRLQQACVSRELYSLLLDVRDGRAVLEHVWTGERREIDCAVAIDCGYRLP
                     EDALWAARPHLDRAGDCIAPRTVYESVLEGRRAAARVERSAA"
     gene            5127..7067
                     /locus_tag="VFE39_15695"
     CDS             5127..7067
                     /locus_tag="VFE39_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin system FadH/OYE family
                     oxidoreductase 2"
                     /protein_id="HET6260080.1"
                     /translation="MMLFSPLRLGPLTLRNRIVFAAHLTNSAEAGRPTAQHAAYYAAR
                     AVGGAGLIITEELSVHPADRPYEKLIGGFDPGAIAGYRRIADAVHAHEVPVLAQLNHN
                     GGQASGMYSRLPVWAPSAVPDPMFREVPHAVTVEEIRELVAGYALVAGHCAAGGFDGV
                     ELQCSQASILRQFLSPLTNRRTDRYGGVLENRVRIVREVVAAVREVLGPERAVGVRLC
                     GDEGIPGGTSLAEAVQTARLLERDGGVDYVNTSTGVATATLHLVEAPMPVPAGYALAV
                     PSAIRAAVSLPVVGVGRFTDPAQAERALREGHCDLVGVVRGQIADPAFATHARAAAPR
                     RIRTCTGCNQECVGRVALNARLGCVVNPRAGHEAVPLPAPARAGRRVLVVGGGPAGLQ
                     AAATAVERGHRVMLCERLPRTGGQVIDAASAPGRAEFAALVHDLLAECLRLGVDVRTG
                     TAVDVSAVEREQPDVVVLATGARPALPVWAGGLDRVVDVRDVLCGRAEPSGSVLVYDE
                     LGFHQATSVAELLAARGCAVEIATPGMVVGQDLGLTLDMERFHRFAHGAGIVMSPDRT
                     VLDAAGAAPGVRLTLLHHTVGAVSEAEYDWVVSAVPAVPEHELWTALRGRGPAVHRIG
                     DCLAPRRAHAAVVEGHRVGVAL"
     gene            7064..7765
                     /gene="mftE"
                     /locus_tag="VFE39_15700"
     CDS             7064..7765
                     /gene="mftE"
                     /locus_tag="VFE39_15700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0140604 - mycofactocin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycofactocin biosynthesis peptidyl-dipeptidase
                     MftE"
                     /protein_id="HET6260081.1"
                     /translation="MTARLGERTWTELQGGMPRTVLVPVGSVEQHGPHLPLDTDARIA
                     AAVALRVADGDPSLLVAPALGYGASGEHQGFPGTLSIGHAALRAVLVELGRSAGAWAE
                     RLVFVNGHGGNLPTVPEAVLQLRDEGRDAAWFGCAAPGGDAHAGRTETSILLALDPVL
                     VRWEAAEAGNTAPLAELLPTLRHGGMAAATPNGVLGDPLGATAEEGTRLLAGMTTALQ
                     HALPRWHLNPRAHLT"
BASE COUNT         1009 a         2850 c         3040 g         1079 t
ORIGIN      
        1 ccgggccgac caggagcgcg tgacctcgac gaacccccgg cgtgcgtacc actgctccgg
       61 ccactcgccg gcggccgcgt ccaggaccac gagatcgcag ccggcctcgg cagccagggc
      121 catcgccgtg cggagcaggg cgtcgccgtg cccacgtcgg cggtgctccg gctcggtcgt
      181 cacggcatcg agcagtgcgg tgccaccgtc gatcttgagg atcgccgagg cgacgagctc
      241 cccgccgctg gggacggcga ggtaacggac gtcgagcacg gcctcctcga gacgctggcc
      301 ggcgacgatc tgggcgagct ccgcagcgcc gatcccgggc acagcgctgc gccagtcggc
      361 ttccaccagg gggcggagcg cctccacgtc gacctgctcg acgcgcgcga ccggttcccc
      421 gccggcgggc gccgccatcc cgaccagctc ctgcacctcc cagccgcgag cggcgagccc
      481 ggcggccgtg gcacccaggt gcgcgcccgt cagcagggcc cagcggtgcg gggcaccgtt
      541 gagagcgcgg tcggcgtcgg cggcgacctg tgtggcgtcc acacggccac tcaccagcag
      601 ctggttgtgc tcgggcgagg cgggcaccga cgcggtgcgg acggcgatac cgcccgccac
      661 gggcacctcg gcggtcgccg tgcgtcgcag cagcgacggt tccagcacgg cgtgccgcca
      721 gcctgggtcg gtgcgctcga tgacgggggc gtcgaacccg ccgtcgccgc gcgggcggta
      781 cggcagcacg gcgagcaccg ccgcggcggg gaccgggccg taggcgtgcg ggaaccgcat
      841 cgacggcggg tcggtcggca gcccgggttc gaagcggatc gggacgccga tccggtcggg
      901 gtcgagcacc agcagcacca ggtcggaacg gccggggaag agccggccgg cgggcagcgc
      961 gacctgctcc ggggtggaca ggtggacgaa ccccacctcg gcgagcgagg ggggcgcgat
     1021 tgcacagacg gcgagcgcag cccgccactc agcgggcgtg gcgaggtgca ggagcggtcc
     1081 ggtcacggcc cgcagcatgc ccgatgcaat atggctgtcc ggaaggagag ccttgtattg
     1141 gcacccggtg ccaattaccg ttcgtcgcga ccaccccgtc gacggcagga ggcccccatg
     1201 tccccgatcg agcagtccga gcccacgacc gaggagcccc tcgtcgagga gacgctcgtc
     1261 gaagaggtct cgatcgacgg catgtgcggt gtttattagc gcggaagcgg ctacgtccat
     1321 gagcgcgcaa gcggatgcgg gtttcgaccc ggatcgggcc taccggtgca gcccccgggt
     1381 cgcgctgcgt cccgagccgt tcggggcgct cgtctaccac ttcggtaccc gcaggctgtc
     1441 gttcctgaag accccgcagc tcgtcgccgt ggtctccggc ttggaacagc acgactccgt
     1501 gcacgccgcg atcgacgcgg cggccgtcga cgaagcgcag cgcccggcgt acctgcgggc
     1561 gctggccggg ctggccacca acggcaccat cgaggagcgt cagtgaagct ggtcgaccac
     1621 ttccagtacg ggctgaactc gccgatctgc ctgacctggg agctcaccta cgcctgcaac
     1681 ctggcgtgcg tgcactgcct gtcgtcctcg ggacgccggg acccgcggga gctgaccacc
     1741 gcggaggcca agggcgtgat cgacgaactg cagcggatgc aggttttcta cgtcaacatc
     1801 ggcggcggcg agccgacggt acggcccgac ttctgggagc tgctcgacta cgcggtgggc
     1861 cacgacgtcg gcgtcaagtt ctcgacgaac gggatcaagc tcgacgagaa gcgggcgcag
     1921 cagctcgccg ccaccgacta cgtcgacgtc cagatctccc tcgacggcgc caccgccgag
     1981 atcaacgacc atgtgcgagg ccccggctcg tacgcgacgg cgatccgggc gctggaaaac
     2041 ctcgccgccg ccgggttcgg gcagccgaag atcagcgtcg tgatgacgcg gcagaacgtc
     2101 gaccagctcg acgagttcgc gacgatcgcg gacaggttcg gcgcgcagct ccgcatcacc
     2161 cggctgcggc cctccggccg tggtgccgac gtctgggacg agctgcaccc caccgcggcg
     2221 cagcagcggc agctctacga ctggctcgtg gcgcacggcg acaacgtgct gaccggcgac
     2281 tcgttcttcc acctttccgc ctacggggaa gcgttgcccg gactcaacct gtgcggtgcg
     2341 ggtcgcgtcg tctgtctgat cgacccggtc ggggacgtct acgcgtgccc gttcgcgatc
     2401 cacgagaact tcctggcagg caacgtccgg tcgcccggtg gcttccagca ggtctggcgg
     2461 cagtcggagc tgttcacgca gctgcggcag ccgcagacgg gcggcgcgtg ccgcagctgc
     2521 ggcttctacg actcgtgcca gggcggctgc atggcggcca agttcttcac cggcctgccg
     2581 ctggacgggc cggacccgga gtgcgtgcgc gggttcggcg agcaggcact cgcggcgcgc
     2641 gaggcggaca cgctggtccc gaggtcggat gtggaccatt cccgttccgc gcgccgtaac
     2701 tcccgcggcc ccgtaccggt gcagatcggg atgccaggcc gtgccgacgg ccagcaggtg
     2761 cgacccgaca gggcctgcga gacgagtccg ctcgccggga taccggagga cgcctccgac
     2821 tcgcggctgc gggtcccggt cgcgggcggc tgaaggcttg ccgctccggt ccgcgtcagc
     2881 gtcgacgcgg ccggagcggc cgtgtgaccg aggcctgcgg agcgtcgacc acgagccgcg
     2941 gaggtgggtg ggaacgtgcg gagcctggtc cggcccgtca cactcggcgg acgggtagcg
     3001 cccagccgcg tgatgttcgg cccgcacgag acgaacctcg cgcgcttccg cgagttctcc
     3061 gaccggcacg tggcctacta cgcccggcgg gcagcaggtg gagcgggcgt gatcgtcacc
     3121 gaggtggcga gcgtccacga ctccgactgg ccttacgagc gcgcaccccg tgccgccgac
     3181 gcgaccggcg gctggacgga ggtggcgaac gcctgccgcc cgtacgggcc gctcgtgctc
     3241 gccgggctcg ggcatgccgg cgcgcagggc agctcggcgt acggacagca ggcgctgtgg
     3301 gggccctccg gagtggccga cgtggtcagc cgcgaggtgc cgatggagct cgagcaaccc
     3361 gagatcgacg agctcgtcac cgggttctcc gccgccgccg agctggccgc aatggccgga
     3421 ctcgacggcg tcgagatcaa cgcgggacag cactcgatcc tgcgccagtt cctgtccggg
     3481 ctcacgaacc gccgcgccga cggctacggc cgggaccggg cccggttgct gcgtgaggtg
     3541 ctggccgccg ctcggtcagg gctcgaccgt gacgggcgcg gcgaccgggt cctcgggctg
     3601 cggttgtgcg ccgacgagct cgcgccgtgg ggcgggatca cgccggagca ggccatcgcg
     3661 accgcatgca cggtggccgg ctcgatcggc tacctggtcg tcgtccgcgg atcggggatg
     3721 tcggtgttcg cgacccggcc ggacctgcac gtgccgcccg ggttcaaccg cctgctgtgc
     3781 gcgactgtcc gcgccgggct cggcgaggcc gtgccggtcg tgctgcaggg cagcgtcgtc
     3841 gacccggggt tcgcgcaggc cgcactcgac gacggggtcg ccgatctcgt cgagatgacc
     3901 cgaagtcaga tcgccgaacc tgatctggtc gcccacgtcc gggcggggac gctggaacgc
     3961 atccggccct gcaccctgtc caaccagcac tccggcgtcc gcgacccgcg caacccgatc
     4021 gtctcggacg aggccgagcc gcgggccggg cacgagaccg tggacccgcc ggtgtcgagc
     4081 cccgcgagtc gcccgtttcc cgcccgcgag tcgcccacaa tctgcccgcg agccgcccgc
     4141 attccgcccg cgagtcgccc attttccgcc cgcgagtcgc ctgcaatcgg cgcgcgagtc
     4201 gcccgttacc cgcccccgag tcgccccgtg gctcgcgctc ctgtgagcga ggaggcgccg
     4261 acgggcgaat cgcgcacgaa cgccgtccgg gtgcggcggt cggtgctcgt ggtgggtggg
     4321 gggccggccg ggatggaggc cgctcgggtg ctgaccctgc gcggacatcg cgtgcggctc
     4381 gccgagcggt cgggccggct cggcggggca ctgctgctcg ccgcggccgt cgcaggtcgg
     4441 gagcgtatgg gtgtgctcgt gccgtggtgg gagcgagagc tggcccggct gggggtcgag
     4501 gtgcagacgg gcgtcgaggt gggcgtcccg gagctggacg gcgcccagcg cgatgggacg
     4561 gcggtgctgc tcgcgaccgg ttcgcggccg gcgccgcgtc cctaccggag cgacggcccc
     4621 gtgctcgacg ccgccgagtt cgaggccgcg gtgctcgccg ccggatcgac ggaggcggtg
     4681 ctggccgggc tgcccggcgg caccgtcgtc gtgcacgacc cgatcggcga ctggacgggg
     4741 gtcgggatcg ccgagcaggt ggtcgccacg gggcgcccga cggcgatagt cacccgcgac
     4801 ccggtggcgg gcgcccagct cggccgcacc ggtgacctcg tccccgccaa cacgcggctg
     4861 cagcaggcct gcgtgtcgcg cgagctgtac tcgctgctgc tcgatgtgcg cgatggccga
     4921 gcagtgctgg agcacgtctg gaccggcgag cggcgcgaga tcgactgcgc agtggcgatc
     4981 gactgcgggt accggctgcc ggaggacgcc ctgtgggcgg cccgccccca cctggaccgc
     5041 gccggcgact gcatcgcccc gcgcacggtg tacgaatccg tgctcgaggg gcggcgcgcg
     5101 gccgcacggg tggagcggag cgcggcatga tgctgttctc gccgctacgg ctcgggccac
     5161 tgaccctgcg caaccggatc gtcttcgccg cgcacctcac gaactcggct gaggccggcc
     5221 gtccgacggc acaacacgcg gcctactacg cggcacgcgc cgtcggcggg gccggactaa
     5281 tcatcaccga ggagctttcg gtccatccgg ctgatcggcc gtacgagaag ctgatcggcg
     5341 ggttcgaccc cggcgccatc gccggttacc ggcggatcgc cgacgccgtg cacgcgcacg
     5401 aggtgccggt gctcgcgcag ctcaaccaca acggtgggca ggcatcgggg atgtactcgc
     5461 ggctccccgt gtgggcgccg tcggcggtgc cggacccgat gttccgcgag gtgccccatg
     5521 ccgtcaccgt cgaggagatc cgggagctcg tcgccgggta cgcgctcgtc gcggggcact
     5581 gcgcggcggg cgggttcgac ggcgtcgagc tgcagtgctc gcaggcgtcg attctccggc
     5641 agttcctctc cccgctgacg aaccggcgca ccgatcggta cggcggcgta ctggagaacc
     5701 gtgtgcggat cgtgcgtgag gtcgtcgccg cggtgcggga ggtgctcggc ccggagcgcg
     5761 cggtcggggt ccggctgtgc ggcgacgagg gcatcccagg cgggacctcg ctcgcggagg
     5821 cggtgcagac cgcgcggctg ctcgagcgcg acggcggggt cgactacgtc aacacctcga
     5881 ccggggtcgc gaccgcgacg ctgcacctcg tcgaggcgcc gatgcctgtg ccggcgggct
     5941 acgcgctggc ggtcccgtcc gcgatccggg ccgccgtgtc gttgccggtg gtcggggtcg
     6001 gccggttcac cgatcccgcg caggccgaac gcgcactccg tgaggggcac tgcgacctgg
     6061 tcggcgtcgt ccgcgggcag atcgccgatc cggccttcgc gacgcacgcc cgcgccgccg
     6121 cacctcgccg gatccgcacc tgcaccgggt gcaaccagga gtgcgtgggt cgggtggcgc
     6181 tcaacgctcg actcggctgt gtggtcaacc cgcgcgcggg ccacgaggcg gtcccgctcc
     6241 cggcaccggc ccgggcgggg cggcgggtac tcgtggtcgg cggcgggccg gcaggactgc
     6301 aggccgcggc gacggcagtc gagcgggggc accgggtgat gctgtgcgag cgcctgccac
     6361 ggacgggcgg gcaggtaatc gacgccgcgt cggcgccggg gcgggcggag ttcgcggcgc
     6421 ttgtgcacga cctgctcgcc gagtgtctgc ggctcggggt cgacgtccgc acgggcaccg
     6481 ccgtcgacgt gtcggccgtg gagcgtgagc agcccgacgt cgtcgtcctc gccacgggtg
     6541 cccggccggc gctcccggtg tgggcgggtg gactggaccg cgtggtcgac gtgcgtgacg
     6601 tgctgtgcgg ccgcgccgag ccgtccggct cggtgttggt gtacgacgag ctcggcttcc
     6661 atcaggccac gtcggttgcc gagctactcg ccgcgcgggg ctgcgccgtg gagatcgcga
     6721 cgccgggcat ggtcgtcggg caggacctcg ggctcaccct ggacatggag cggttccacc
     6781 gctttgcgca cggggccggc atcgtgatgt cgcccgaccg caccgtcctg gatgccgcgg
     6841 gtgctgcacc cggcgtccgg ctcacgctgc tgcaccacac cgtcggcgca gtgtccgagg
     6901 ccgagtacga ctgggtggtc tccgccgtgc cggcggtgcc ggagcacgag ctgtggaccg
     6961 cgttgcgcgg tcgtggcccg gccgtgcacc ggatcggcga ctgcctggcg ccccgcaggg
     7021 cccacgccgc cgtcgtcgag ggacaccggg tgggggtggc actgtgacgg ctcggctcgg
     7081 tgaacgcacc tggacggagc tgcagggtgg gatgccgcgc accgtgctgg tgccggtggg
     7141 ttcggtggag cagcacggac cgcacctgcc gctcgacacc gacgcgcgga tcgcggccgc
     7201 cgtcgccctt cgcgttgctg acggcgaccc gtccctgctg gtcgcgccgg cgctgggcta
     7261 cggcgcctcc ggcgagcacc agggcttccc gggcaccctc tcgatcgggc acgcggccct
     7321 gcgcgcagtg ctcgtcgagc tgggcaggtc ggcgggggcg tgggcggagc ggctggtgtt
     7381 cgtcaacggg cacgggggaa acctgccgac agtgcccgag gccgtcctcc agctgcgcga
     7441 cgagggccgc gacgcggcct ggttcggctg tgccgcgccg ggaggggatg cccatgcggg
     7501 gcgcacggag acctcgatcc tcctcgcgct cgatcctgtc ctcgtccgat gggaggcggc
     7561 cgaggcgggc aacacggcgc cgctggcgga gctcctcccg accctgcgcc atggtggcat
     7621 ggccgcggcc actccgaacg gcgtgctggg tgatccgctg ggcgccaccg ccgaggaggg
     7681 cacccgtctg ctggccggta tgacgacggc gctgcaacac gccctgcccc gctggcacct
     7741 gaacccccga gcccacctca cctgacccgc gagtcgcccg ttgggggccc gtgagtcgcc
     7801 cggatcccgc ccgcgagtcg cccgttctgg gcccgcgagt cgcccggatc ccgcctctga
     7861 cttgcccgtc cccgacagtg agttgccgga accgcccggc gagtcgtccg tatcgcgtgc
     7921 ggcagagcag gtcgcgcagg cgcgggttgg gcgactctca tgggcgggaa gggcgcga
//
LOCUS       DASRSC010000320         1935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1771078,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000320 DASRSC010000000
VERSION     DASRSC010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1771078"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..87
                     /locus_tag="VFE39_15705"
                     /pseudo
     CDS             10..87
                     /locus_tag="VFE39_15705"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            216..815
                     /locus_tag="VFE39_15710"
     CDS             216..815
                     /locus_tag="VFE39_15710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260082.1"
                     /translation="MGGFKGSHSEYVRIPFVDHTAFTVPDGVDDLSAVFASDSAPTGW
                     MGAHLGGVQPGDVVPVWGCGAVGQMAARSAMILGAERVIAGTPDGPRAHLARSDPRVP
                     QGRKRVRPRCLRRPGRPLPHGRPHEQALTLRGAQQHGERYIPMLLDRIAAGELKTAHL
                     ATHPMSLEQGARGYDLFKNKKQNCLRAVFSPDGFSPNGG"
     gene            1025..>1935
                     /locus_tag="VFE39_15715"
     CDS             1025..>1935
                     /locus_tag="VFE39_15715"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET6260083.1"
                     /translation="MRVAVVGAGVVGLSTTAALLQGGVDVVCYERTGVPMAQRSAGSS
                     RIFRLAHPTPDLVRLARAARAGFGRWEQAAETSMIVPSGCVISGDRAQGWAAAMAAAV
                     APHEIVDAPSGELRLPALTPPSGALLDPSGGVIDVDAVRAYLIEVTRAAVAGVQVYAI
                     EDTPAGASVWSPAGEARFDAVVLAAGVGTSPLAAQVGIYTPPALAHHVRFTFPIDPSV
                     AWQSWIDKPTAGLATYQHQSGPGRWAVGGEIDPELTAWEVGRDVSASASYNAVLTYAR
                     EHLAVEPRIVDSLYCVTTPGLGDGFQVR"
BASE COUNT          270 a          653 c          695 g          317 t
ORIGIN      
        1 cacgagttca tgggcgagat cgtcgatgtc ggcccggagg agcgcaagca caagcccggc
       61 gaccgggtgc tcgtctgctc gttcatcgcg tgcggtcgct gctggtactg cgagcacgag
      121 ctgtactcct gttgcgacaa cagcaacacc aacccggcga tcaccgaaag catgtgggtc
      181 aggctcccgg aggcatcttc ggttagtcgc atgcgatggg tgggttcaag gggagccact
      241 ccgagtacgt ccgcatcccc ttcgtcgatc acacggcgtt cacggttccc gacggcgtcg
      301 acgacctgtc ggcggtcttc gcctccgact cggcgccgac cggctggatg ggcgcccacc
      361 tcggcggcgt gcaacccgga gacgtggtcc ccgtctgggg ctgcggagcg gtcggccaga
      421 tggcggcccg ctcggcgatg attctcggcg ccgagcgcgt gatcgcgggt accccagacg
      481 gaccgcgggc acaccttgcg cgaagcgatc ctcgcgtgcc gcaagggcgg aagcgtgttc
      541 gtcctcggtg tcttcgccgg cctggtcgac cacttcccca tgggcgccct catgaacaag
      601 cccttaccct gcggggcgcc cagcaacatg gtgagcgcta catcccgatg ctgctcgatc
      661 ggatagccgc gggcgagctc aagactgctc acctcgcgac ccacccgatg tcattggagc
      721 agggcgcacg gggctacgac ctgttcaaga acaagaagca gaactgcctg cgcgcggtgt
      781 tcagccccga cgggttcagc cccaacggcg ggtagccccg cggattccgg cgccacgaca
      841 tgcgccggta cttcgagcac gtcggccgcg acctcgccga cgcggccgag tgctggcgct
      901 ctgtcttcac caggcgctca ccggcttgcc gacgggcaac ccgccgggtg ctggcctcac
      961 ccggcgcacc cctcagctcg gatctcgcgc gttttcgggg tctcgggcat tacgctcggt
     1021 tcacgtgagg gtcgcggtcg tcggtgccgg cgtggtcggg ctgtccacta cggccgcgct
     1081 gctgcagggc ggcgtggacg tggtgtgtta cgagcggacg ggcgtgccga tggcgcagcg
     1141 gtccgccggg tcgtcgcgga tcttccggtt ggctcatccc accccggacc tggtgaggct
     1201 cgcgcgggcg gcccgggcgg ggttcggtcg gtgggagcag gctgcggaaa cgtcgatgat
     1261 cgtcccgagt ggctgtgtga tcagtgggga ccgtgcgcag ggctgggcag cagccatggc
     1321 tgctgccgtg gcaccgcacg agatcgtcga tgcgccctcg ggcgagctgc gactacctgc
     1381 gctcacgccg ccgtccggag cgctgctcga cccatcgggt ggcgtgatcg acgtcgacgc
     1441 ggtgcgggcc tacctgatcg aggtgacccg ggctgccgtc gccggggtgc aggtgtatgc
     1501 gatcgaggac acccccgccg gcgcaagcgt gtggagtccg gccggggagg ctcgcttcga
     1561 cgcggttgtg ctggcggcgg gagtcgggac atcgccgctc gccgcgcagg tcggcatcta
     1621 caccccgccc gcgctggcgc accacgtccg gttcaccttc ccgatcgacc cgtcggtggc
     1681 gtggcaaagc tggatcgaca agccgaccgc cgggttggcc acgtaccagc accagagcgg
     1741 tcccggaagg tgggcggtgg ggggagagat cgatcccgag ctgacggcct gggaggtggg
     1801 tcgcgacgtg tcagcgtccg cgtcgtacaa cgccgtcctc acgtacgcgc gcgagcacct
     1861 cgcggtcgag cctcgcatcg ttgacagcct gtactgcgtc acgacgcccg gtttgggcga
     1921 cgggttccag gtccg
//
LOCUS       DASRSC010000321        23316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4392651,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000321 DASRSC010000000
VERSION     DASRSC010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 23316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23316
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4392651"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..339)
                     /locus_tag="VFE39_15720"
     CDS             complement(73..339)
                     /locus_tag="VFE39_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter complex subunit
                     F"
                     /protein_id="HET6260084.1"
                     /translation="MTIVFAVVLGMLGLAAALVIVRLLLGPETLDRIAALDAFVVLIV
                     AGAAVYVAYYEDGSNIPLLAAVALVGFVGSATAMRLVERRERHR"
     gene            complement(336..920)
                     /locus_tag="VFE39_15725"
     CDS             complement(336..920)
                     /locus_tag="VFE39_15725"
                     /inference="COORDINATES: protein motif:HMM:NF006521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HET6260085.1"
                     /translation="MTVHDDRSARGPASRLRARLPGVVWLTLVWVLLWGTFTPLSIVG
                     GVVVGVLVTALFRMPRASTRLPVRPLRLLGLVVYLGYDLVVSGLEVSRQILRHGPHAR
                     GAIVVVPLLTRSDQVATAMANALSLAPGMMALQTDQDHDVWYVYALGPRDRAGVERAR
                     LRTMDMQRRVLAAFGSPAELAEAERRIAAAGARR"
     gene            complement(917..2449)
                     /locus_tag="VFE39_15730"
     CDS             complement(917..2449)
                     /locus_tag="VFE39_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit D"
                     /protein_id="HET6260086.1"
                     /translation="MTTVLTLPVLLPMLGAAVTVLGSRRAALQRVVGVVVLSVVAVLA
                     GVLLVVADERGPIVTQLGGWAAPVGIALVADRLSALLLLVSTLVTLAVLVYAIDQRIA
                     DYGRGTASTTFHPMYLMLCAGVSLAYLTGDLFTLFVAFELMLTASYVLITRRTNASRI
                     RAGMTYVTVSLLSSLLFLTAIALIYAATGTVNLADLAGRIGTLPSGLRSVLGLMLLVV
                     FGIKAAIVPLHFWLPDSYPNAPGPVAALFAGLLTKVSFYALLRTQTLLFTRDEPWTLL
                     LVLAVATMLVGALGALVQNDLNRLLSFLLVSHIGFMLFGLAVFDAAGVAGAALYAAHH
                     ITVLATLFLVSGLITRRTGTVALDRMGGLARSAPWLAVLFAIPALTLAGMPPTAGFVA
                     KLALLEAGAAAGVDTRIVAGVVIMASLLTLYALVRVWVRVFWGKPCAPLPDEDDADEV
                     VVGTARSSVPMYVATGLLVAVSLAIAVFAGPLTALTARAGADLVDPVSYRTAVLGGRA
                     SR"
     gene            complement(2446..2934)
                     /locus_tag="VFE39_15735"
     CDS             complement(2446..2934)
                     /locus_tag="VFE39_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter subunit C"
                     /protein_id="HET6260087.1"
                     /translation="MTMTMTVTMNPVTINVVMALTVGVLYAVGFTLLLSRSLMRVLIG
                     VVVLGHGTNLLLQLAGGPPARPPIVGTAPPESFADPLPQALALTAIVITFALTTFLLA
                     LGYRAFVLVGHDEVQDDVEDRRIARQKVPSAPLEAGDGEEDVEGADDEVEAAAARGRD
                     GS"
     gene            complement(2934..3485)
                     /locus_tag="VFE39_15740"
     CDS             complement(2934..3485)
                     /locus_tag="VFE39_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005455561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HET6260088.1"
                     /translation="MTTTRDESRDPVPFEEWDRPREAWLLSGGCRRPGQRSLLLEVTT
                     RAVFPTVLVFSLYLLLVGHYGPGGGFSAGLVAGLAFVLRYIAGGSDDLGAVVRVPAPV
                     VIGTGLVVALLTALAPVAWGAPVLSATKLAVALPLLGRVELQTSVFLDVGVYVLIIGV
                     VLDLLRTLGTGIEHDMHDAGEDP"
     gene            complement(3482..5845)
                     /gene="mbhE"
                     /locus_tag="VFE39_15745"
     CDS             complement(3482..5845)
                     /gene="mbhE"
                     /locus_tag="VFE39_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen gas-evolving membrane-bound hydrogenase
                     subunit E"
                     /protein_id="HET6260089.1"
                     /translation="MLTLVVAHLAVAALLPLVSARHRRAAFGVAAVLPAATLLWALAN
                     TGPALHGGLTATTAWAPTLGLTLSFRLDALALAMVVLVSGIGAVILVYCIGYFGTDSP
                     EAPRNAALLLAFAGAMLGLVLADDLLTLYVFWELTSIASFLLVGQGGEQREERRAAVQ
                     ALLVTVFGGLAMLLGFVLLGEAAGTYVIPEILAAGQAGRLHGGVVTAALLLILLGAFT
                     KSAQLPFHPWLPAAMVAPTPVSAYLHAASMVKAGVYLVARLAPGFSDLAVWWVPVVVI
                     GLATMVVGGWRALSATDLKQLLAFGTVSQLGFLIVLLGSGGPLGEISALAGMALLLAH
                     GLFKAPLFMTVGAIDHATGSRDLRKLSGLRTAMPGTAAAAALAAASMAGLPPLLGFVG
                     KEAAFEGFLDEGGLRGWIIALGLVLGSTITAAYSARFLWGAFARKPGIEPTPVHHRPG
                     ALLTVPIWICAVAGLVLGIAYPVVDALGASYAAPYPDVSGYHLALWHGLGLPLLLTAV
                     ALGGGMVLHARRDRVTALASRSPLDAQRGYELTIAAIARVALGVTGRLQVGSLPAYLA
                     TILVAFVLLPGAAAVLGGGWPAGHRLYHSVMQVPLAIVVVIAALAVARAKRRFTAVLL
                     VGVIGYGVGGLFIVDGAPDLALAQFLVETLSLVAFVFVLRRLPADFTEERPPRRVRVP
                     KAVLGTVGGAAVAAFAVVLSGARTEPSSTIGEFVRLAPAEAGANNVVSAIIVDFRALD
                     TVVEIGVLFIAAAGVASLVLATRYDRRGRRRVESGQSSPQHEEDVLG"
     gene            complement(6310..6525)
                     /locus_tag="VFE39_15750"
     CDS             complement(6310..6525)
                     /locus_tag="VFE39_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260090.1"
                     /translation="MFDLRSIIALLFGVYGIILTVMGLFFESAAELQKAGGIDINLWS
                     GIVMILVAALFALWAWLRPLVPPGERR"
     gene            complement(6639..8315)
                     /locus_tag="VFE39_15755"
     CDS             complement(6639..8315)
                     /locus_tag="VFE39_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HET6260091.1"
                     /translation="MGVLAQTDLRLDTTAIDFVFVVFYFVLVLGIGVMARRSISSSLD
                     FLLSGRSLPAWVTGLAFVSANLGAIELIGMMANGAQYGVATFHYYWIGAIPAMVFLGI
                     VMMPFYYGSKARSVPEFLRKRFNSTTQRVNAVTFAVASVLIAGVNLFALGLVLEALLG
                     WSLNLAIPIAALVVLSYTFLGGLSAAIYNEVLQFFVILALLVPLTVAGLARVGGWDGL
                     KQSVSALPPGPEQVSSWPGTALTEIANPFLSVLGIVFGLGFVLSFGYWTTNFAEVQRA
                     LSARSMSAARRTPIIAAFPKALIPLVVVVPGMIAGVLVPQLTTLKQGGATDVTYNNAL
                     SLLMNEVLPNGILGVALAGLLASFMAGMAANVSSLNTVFTYDIWQDWVRPGRPDDYYL
                     RVGRTVTVVGCVLAIGTAFIAGSFQNLMDYIQTLFSFFNAPLFAIFILGLFWKRMTGT
                     AGWTGLIAGTVSAVTVDALVRSGVIAVSSQAGSFIGASAAFVVGIAVAVVVSTVTAPK
                     PDAELVGLVWSLTPRDRMRTGEDEGSWWQSPVVLGAIVIALTIVLYLLFA"
     gene            complement(8736..10904)
                     /locus_tag="VFE39_15760"
     CDS             complement(8736..10904)
                     /locus_tag="VFE39_15760"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HET6260092.1"
                     /translation="MTDLQVDPALRAFVADELLPGVDLDPEWFWSTCAALDERFAGRT
                     EELLARRDELQERIDAWHRERGGGDDESFVDAYEAFLTDIGYLLPPASPQVRVSHVDA
                     EIAEIPGPQLVVPSTVPRYALNAVNARWGSLFDALYGTDVLPLDHELERGYDERRGAQ
                     VIAEADRLLDELFPLATGSHADATAYRVQDGALVVETKDGDTGLADPGQFAGRRSGSD
                     GGGDDDGRPAAVRAVLLRRNGLHVELTIDPEHRVGRQHHADVADVALESAVTTIVDFE
                     DSVATVDGPDKAEAYRTWLGLMTGTLTSTFEKGGETVERKVNGDRSYTAPDGSELTLP
                     GRSVLLVRNVGHHMRTDAVRTADGDPIAEGVLDALVSATAALYDLRGLGRYSNSRAGS
                     VYIVKPKQHGPDEVALTVELFAAVEEALGLEPATLKIGIMDEEKRTSLNLEACIAQAQ
                     DRVIFVNTGFLDRTGDEIHTDLEAGPVVRKNDMKTTPWLLAYEDRNVDVALRAGFVGQ
                     AQVGKGMWAKPAAMREMLETKGAQPEGGANTAWVPSPTAATLHALHYLRTDVLGRQRE
                     LAGRETDRRELLRVPLLTGELTDDEKRHELETNAQSILGYVVRWVGLGIGCSTVPDLA
                     GVGLMEDRATLRISSQLIANWLHHGLTDEAQVRETFARMAALVDEQNANEPGYEPMAK
                     DLDNSPSFQAALDLVLTGRSEPNGYTERALTTWRQKAKTS"
     gene            complement(11021..11155)
                     /locus_tag="VFE39_15765"
     CDS             complement(11021..11155)
                     /locus_tag="VFE39_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260093.1"
                     /translation="MGPRRKLQLSLLLAAAVALLLALTGVVGPAAATPVPAPAAVVSS
                     "
     gene            11593..13047
                     /locus_tag="VFE39_15770"
     CDS             11593..13047
                     /locus_tag="VFE39_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HET6260094.1"
                     /translation="MPLAAGVRAAFERMLDPADVISNPVQCRAYECDGLTGYRVVPEL
                     VLLARDAAQVAAAVRVCAEHRVPFVARGAGTGLSGGALPVADGVVISLARLKRVLEID
                     PQNRRAVVEPGVTNLEITAAAAPHGLYYAPDPSSQQVCTIGGNVAENSGGAHCLKYGF
                     TTNHVLSCEVVLPDGSIVTLGSDTGEQAGPDLRGVFLGSEGTLGITTKITVRLLRTPE
                     SVRTLLADFPSVAAAGDVVSDIVAAGIVPAAVEMMDTLAIEAAEEAVHAGYTVGVPAA
                     LVVELDGPAAECDVQFEQVKEICDRHGCTRLHIAGSPEERAAIWRGRKAAFAAVGRIS
                     PDYFVQDGVVPRTRLAEVLDRIAGMGRDAGIRVANVFHAGDGNLHPLVLYSAAAGETE
                     RAEKLSGQIAELCVELGGSLSGEHGIGTDKACSMPKMFSRDDLAVMQRARSAFDPHGI
                     CNPGKVLPTPRLCGERPGKYKPHPLEESGVIERL"
     gene            13077..14264
                     /locus_tag="VFE39_15775"
     CDS             13077..14264
                     /locus_tag="VFE39_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6260095.1"
                     /translation="MLRPTDLRSVRDALLDTAGTVSIAGAGTAADWAGSLEPVEAVLD
                     TTGLTGVITHNPGDMTVSVLAGTPLRALTEELAPHGQHVSYDAARVADGATVGGLIAT
                     ADAGPAALVFGSLRDLVIGVTIVLADGTVARSGGHVIKNVAGYDLAKLLHGSYGTLGV
                     LVEVVLRLHPVPKRTATLVVDCSLADAAEQAARVLGGQYEPAALEWVSAGAGDGQLLL
                     RVEGTESALSARLQQLREVLGAGAQEAAEPAGERPARRAGADESAARAADPWAHHATL
                     VRGTSDHAVLRIGALPSALPGLLADLPATTVTAGLGTGVATVSLAPEAVADAHAVVHA
                     AGGTSVLRARPAASDAPAWGPPPSAIAVLRAVKAEFDPRGRFGPGRFAPWPLTRNEEK
                     GSR"
     gene            14261..15622
                     /locus_tag="VFE39_15780"
     CDS             14261..15622
                     /locus_tag="VFE39_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HET6260096.1"
                     /translation="MTTPAGSAETNLPQTGPSSFDDRRPPERELLDDCVHCGFCLPTC
                     PTYQLWGEEMDSPRGRIYLMDLAAKGEIDLDGPLTEHIDRCLGCMACVTACPSGVQYD
                     RLLESVRPQIERNVERAPSDRLFRNAIFALFPYKRRLRAAAVPGALYQRLRRMPAIAK
                     LAGKLPGRLAAMESLLPPVSVREAFARLPVHTPAVGERRARVALLSGCVQDVFFHRVN
                     EATVRVLAAEGCDVLVPADQQCCGALELHSGREDSALVRARRAIAVFETLDVDHVVTN
                     VAGCGSSMKEYGHLLSDDPEWAERAAAFSARVRDVHEVLAELGPRAPRHPFRGRVAYH
                     DACHLGHAQKVRAQPRAVLRSIPDLELVDLPEAELCCGSAGIYNMIMPDAAADLGARK
                     AANVRSVEPDVIVTANPGCLLQIGKYLGAAGGAADGGGPIPLLHPVQLLDAAIRGVPV
                     PRR"
     gene            15751..16311
                     /locus_tag="VFE39_15785"
     CDS             15751..16311
                     /locus_tag="VFE39_15785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020643298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6260097.1"
                     /translation="MSIDWTRMLVEQLEWHWQEHLRPRLDGLADDEYFWEPAPGAWNV
                     RPRGTSTAPVQAGCGEFTVDFAFPEPVPPPVTTIAWRLAHLIVGVFGARAGSHFGGRP
                     IDYESFDYAGSAGAALRQLDEVHHAWVAGVRGLDAAALERPVGSAEGPFAEHPMAELV
                     LHINREAIHHGAEVLLLRDLYRTTGT"
     gene            16394..18079
                     /locus_tag="VFE39_15790"
     CDS             16394..18079
                     /locus_tag="VFE39_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HET6260098.1"
                     /translation="MWATVDLLILSSAHPPAGVVLAAYEQNPEAVGGSLGLSALVSVI
                     PLAVVLVLLGVLRVRAPWAALAGLAAALVVAIIGFDMPVGISLSAAAHGATYGLFPIL
                     WIVVNALWVFNMTVATGHFDVLRRSFGALSIDQRTQAILVAFCFGGLLEALAGFGAPV
                     AITAVMLVALGFQPLKAAVVALVANTAPVAFGAMGVPVITLARVTGLPLDQVASMVGR
                     QTPLLALFVPLVLVFIVDGRRGVREAWLPAVACGVVFAIGQFLTSNFFSVELTDIVAS
                     LLGAIVVFVLARVRPQAVWAARQSTMAAAGGPGTAAKPDSPTEVRKAYAPYVIIVVVF
                     ALAQLPGLKPLIDSLKIAFKWPFLDVVGIDGKPHSTTMFSLTWLSSAGTLMVIAGVIV
                     AALLGVGARRALATWGRTVVDLRWAILTVAAVLALAYVMNASAQTTAIGLFVAGAGGA
                     LAFLSPWLGWLGVAVTGSDTSANALFGSLQVTAAQGAGLPPELLAGANSSGGVLGKML
                     SPQNLTIAAAAVKLEGQEGVLLRKVIGWSLGMLVVLSVLVALQATPVLGWMLP"
     gene            complement(18154..19140)
                     /locus_tag="VFE39_15795"
     CDS             complement(18154..19140)
                     /locus_tag="VFE39_15795"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HET6260099.1"
                     /translation="MPTSFPPPTGDAEAENTLAAIGEFGLISRVTRGRTQPAGTLLGP
                     GDDAAVVAAPDGRVVACTDVLVEGVHFRLDWSAADQVGRKAAAANLADVAAMGAVPTA
                     LLVGLACSKDTPVETLTGLADGLWAEADLAGVGVVGGDVAASPTLVLSVTALGSLEGR
                     SPVTRAGARPGEALALCGRIGWAAAGLAVLSRGFRSPVAVVGAHRVPEPPYAAGPQAA
                     LAGATSMIDISDGLVADLGHVARASGVVLDVRSKAFEVPARLVDVGAALGVDPLEWLL
                     TGGEDHALAATFPGPVPDGWTAIGTVRAGDPRVLVDGRPYESGTAGWDHFAR"
     gene            19216..19452
                     /locus_tag="VFE39_15800"
     CDS             19216..19452
                     /locus_tag="VFE39_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HET6260100.1"
                     /translation="MVQAYILVQTEVGKAAAVAAEISGITGVVSAEDVTGPYDVIVRA
                     EADDVDLLGQLVVAQVQGVGGITRTLTCPVVHLS"
     gene            19497..20024
                     /locus_tag="VFE39_15805"
     CDS             19497..20024
                     /locus_tag="VFE39_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003091996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3515 domain-containing protein"
                     /protein_id="HET6260101.1"
                     /translation="MIAIAIPLIFAVVVAAISITMRGRGAAEPPRPDTGPLAVVPVDA
                     PDAAGPECTILLAALPADLSGAGGTLHERPLADPAPPGVRAWAAAPQPVVLRCGLPRP
                     VELTPTAALLEVNGVRWLKLDDGVPDPDVASYVAVDRPVYVVLTAPLDAGSGPLQQVS
                     DLVRNSLPATEVKVR"
     gene            20059..20751
                     /locus_tag="VFE39_15810"
     CDS             20059..20751
                     /locus_tag="VFE39_15810"
                     /inference="COORDINATES: protein motif:HMM:NF014762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET6260102.1"
                     /translation="MVTMSRATEDYLKAIYHLAHRGEPVTTGLLAHELGVSSPSVSSM
                     VKRLEDGDLLTRPDHRSLHLTDSGERAALRVVRRHRLLETFLARMLDVPWDEVHAEAE
                     LLEHALSDRLEERIDAVLGHPTHDPHGDPIPPRDGPHEESWGEALATVPPGSRFLVER
                     VSDRDSGALRYLGDLGVFPGVVVTVEEQAPFGGPRWVRIGGERHPLGGPLTQLVHGHV
                     VGEAHVVEKAEQ"
     gene            20748..22010
                     /locus_tag="VFE39_15815"
     CDS             20748..22010
                     /locus_tag="VFE39_15815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HET6260103.1"
                     /translation="MTATKGTAPSVDEIRARGGLRSRLAFFGPAIVAAIAYVDPGNFA
                     TNFSAGAGFGYLLLWVIVAANLLAMLIQTLSAKLGLATGRNLPEMCREQFPRPVTRVM
                     WVQAELVAIATDLAEVIGGAIALNLLFGIPLLTGGVITGIVAFALLGLQARGYRPFEL
                     AIAGLFGVILLGFLSTVLRIGIDVGDAAAGLVPRFEGTDSLLLATGILGATVMPHVIY
                     LHSALTNARIPATSVSDRRFLLRYQQIDVLIGMGLAGLVNASMLLISAALFFGTDVPA
                     TDTLGGVYAGLARVLDQPAAFAFALALLASGFASSGVGTYAGQVVMQGFIQRRIPLLL
                     RRLLTISPALVVLGIGWDPTRALVLSQVVLSFGIPFALVPLVLLTRRRDVMAELVNRR
                     LTTAVAALAAAVIIALNAFLIYRTVTGG"
     gene            complement(22027..23139)
                     /locus_tag="VFE39_15820"
     CDS             complement(22027..23139)
                     /locus_tag="VFE39_15820"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HET6260104.1"
                     /translation="MSGDKVRVAVVFGGRSSEHAISCISAGSVLSYLDRERFEVVPVG
                     ITTSGAWVLGTDDPRELAISDRVLPSVDESRTALALPGDPTRGGLVVLDPDRAGEVFS
                     RVEVVFPVLHGPFGEDGTIQGLLEMAGVPYVGAGILASAAGMDKEFAKKLLAAEGLPV
                     GDLVVLRPGTATLTSEQRDRLGLPVFVKPARAGSSVGITRVTDWAVLDGAIALAREHD
                     PKVLVEAAIVGREVECGVLEFPDGTVRASVPAEIRIVGTGAGGSTPEFYDFQAKYLDD
                     VCEFDIPAKLDDAVSDRLREMAVAAFRALDGQGLARVDFFLGPAGELTINEVNTMPGF
                     TPISMFPRAWAVTGIDYPTLLTTLVETAIARGTGLR"
BASE COUNT         3466 a         8463 c         8215 g         3172 t
ORIGIN      
        1 cgagcgcgcc gagcaggctc gacagggcgc cgaggagcag gaacgccgcg gagaccgtgt
       61 ccagcacggt gttcaccggt gccgctccct gcgctccacc agccgcatcg cggtggccga
      121 accgacgaag ccgaccagcg cgaccgcagc cagcagcggg atgttggagc cgtcctcgta
      181 gtaggccacg tacaccgcag cgccggcgac gatcagcacg acgaacgcgt cgagcgccgc
      241 gatgcggtcc agcgtctccg ggccgagcag caggcggaca atgacgaggg cggcggcgag
      301 cccgagcatc cccagcacga ccgcgaacac gatggtcacc gcctcgcccc cgccgccgca
      361 atccgccgct ccgcctccgc cagctccgca ggcgagccga acgccgccag gacccgccgc
      421 tgcatgtcca tcgtgcgcag ccgggcgcgc tcaacgccgg cccggtcgcg cgggccgagg
      481 gcgtagacgt accagacgtc gtggtcctgg tcggtctgca gcgccatcat gcccggtgcc
      541 agcgacagcg cgttggccat ggcggtggcg acctggtcgg acctcgtgag caacggcacg
      601 accacgatcg cgccccgggc gtgcggcccg tgccgcagga tctgccggct cacctccagg
      661 cccgacacca cgaggtcgta cccgaggtag acgacgagtc cgagcaggcg cagcggccgc
      721 acgggcagcc gggtcgacgc ccgcggcatc cggaacagcg ccgtgacgag caccccgacc
      781 accaccccgc cgacgatgct cagcggcgtg aaggtgcccc acagcagcac ccacacgagc
      841 gtcagccaga ccacgccggg cagccgggca cggagcctgg acgcgggtcc ccgcgcgctc
      901 ctgtcgtcgt ggacggtcat cggctcgccc gcccgcccag caccgcggtg cggtacgaca
      961 ccgggtccac caggtccgcc cccgcgcgag cggtgagggc cgtcaacggt cccgcgaaca
     1021 ccgcgatcgc caggctgacc gccaccagca gtccggtcgc gacgtacatc ggtaccgacg
     1081 acctggccgt gccgacgacg acctcgtcgg catcgtcctc gtcgggcagc ggggcgcacg
     1141 gctttcccca gaacacccgc acccacaccc ggaccagcgc atagagggtc agcagactgg
     1201 ccatgatcac cacgccggcc acgatccggg tatccacccc cgccgccgcc ccggcctcca
     1261 gcagtgcgag cttggcgacg aaccccgcgg tcggtggcat gccggccagc gtcagcgccg
     1321 gtatcgcgaa aagtacggcg agccagggcg cgctgcgggc gagcccgccc atcctgtcca
     1381 gtgccacggt gccggtgcgg cgggtgatca gaccgctcac caggaacagc gtggcgagca
     1441 cggtgatgtg gtgggccgcg tacagcgccg cccctgccac gccggcagcg tcgaacacgg
     1501 ccagcccgaa cagcatgaac ccgatgtggc tgaccagtag gaacgagagc agacggttga
     1561 ggtcgttctg gaccagcgcg cccagcgcac cgaccagcat cgtcgcgacg gccagcacga
     1621 gcagcagcgt ccacggctcg tcgcgggtga acagcagcgt ctgggtgcgt agcagagcat
     1681 agaagctcac cttggtgagc aggccggcga acagcgcggc gaccgggccg ggcgcgttgg
     1741 gatagctgtc gggcagccag aagtgcagtg ggacgatcgc cgccttgatc ccgaagacga
     1801 ccagcagcat caggccgagc accgagcgca ggccggacgg aagcgtcccg atccggccgg
     1861 cgaggtcggc caggttcact gtgccggtgg cggcgtagat cagggcgatc gccgtgagga
     1921 acaacagcga cgacagcagg ctgacggtca cgtacgtcat gccggcccgg atccgcgacg
     1981 cgttcgtgcg gcgcgtgatc aggacgtagg acgcggtcag catcagctcg aacgccacga
     2041 acagggtgaa caggtccccc gtcaggtagg cgagcgacac cccggcgcag agcatcaggt
     2101 acatcgggtg gaaggtcgtg ctcgcggtgc cgcgcccgta gtcggcgatc cgctggtcga
     2161 tcgcatagac cagcactgcg agcgtcacca acgtcgagac gagcagcagc agggccgaga
     2221 gccggtcggc caccagcgcg atgcccaccg gggcggccca gccgccgagc tgcgtcacga
     2281 tcgggccacg ctcgtcggcc accaccagca gcacaccggc gagcaccgcc accaccgaca
     2341 ggacgacgac acccaccact cgctgcagcg ccgcccgccg gctgccgagc accgtgaccg
     2401 ctgccccgag catgggcagc agcaccggca gcgtcaggac cgtggtcacg agccgtcccg
     2461 accgcgcgcc gctgccgcct cgacctcgtc gtcggcgcct tcgacgtcct cctcgccgtc
     2521 acccgcctcg agtggcgcgg agggcacctt ctgccgcgcg atccgccggt cctcgacgtc
     2581 gtcctgcacc tcgtcgtgcc cgaccagcac gaaggcccgg tagcccagcg cgagcaggaa
     2641 cgtcgtgagc gcgaaggtga tcacgatggc cgtcagggcg agtgcctgcg gaagcgggtc
     2701 ggcgaacgac tccggcggcg ctgtcccgac gatcggcggt cgcgcaggcg ggccgccggc
     2761 gagctgcagc agcaggttgg tgccgtggcc caggacgacc acgccgatca gcacgcgcat
     2821 cagggaccgc gacagcagca gggtgaagcc gaccgcgtag agcactccga cggtcagcgc
     2881 catgaccacg ttgatcgtga ccggattcat cgtcaccgtc atcgtcatcg tcatcaggga
     2941 tcctccccgg cgtcgtgcat gtcgtgttcg atgcccgtgc cgagcgtgcg cagcaggtcg
     3001 agcaccacgc cgatgatcag gacgtacaca ccgacgtcga ggaacacgct ggtctgcagc
     3061 tcgactcggc cgagcagcgg cagcgccacg gcgagcttcg tggccgagag caccggtgcg
     3121 ccccacgcca ccggcgccag cgccgtcagc agtgcgacca ccagcccggt cccgatgacg
     3181 accggggccg ggacccgcac cacggcgccg aggtcgtcgc tgccgcccgc gatgtaacgc
     3241 agtacgaacg cgagcccggc cacgagaccg gcggagaacc cgccccctgg cccgtagtgc
     3301 cccaccagca gcaggtacag ggagaagacc agcacggtcg ggaagaccgc ccgggtggtc
     3361 acctccagca ggaggctccg ctgcccggga cggcgacagc cgccggagag cagccacgcc
     3421 tcccgcggcc ggtcccactc ctcgaagggg accgggtcgc ggctctcgtc ccgcgtggtc
     3481 gtcatccgag tacgtcctcc tcgtgctgcg ggctgctctg accgctctcg accctgcgcc
     3541 tgccccgccg gtcgtagcgg gtggccagca cgaggctcgc caccccggcc gcagcgatga
     3601 acaggacgcc gatctccacg acggtgtcca gcgccctgaa gtcgacgatg atggcactga
     3661 ccacgttgtt cgcccccgcc tctgcaggcg ccaaccggac gaactcgccg atcgtcgagg
     3721 acggctcggt gcgtgcgccg gacagcacca ccgcgaacgc cgcgacggcg gccccaccca
     3781 cggtccccag caccgccttg ggcacccgca cccgccgcgg cggccgctcc tcggtgaagt
     3841 cggcgggcag cctgcgcagg acgaagacga acgccacgag cgacagcgtc tcgacgagga
     3901 actgcgccag cgccaggtcc ggggcaccgt ccacgatgaa cagcccgccc acgccgtacc
     3961 cgatcacccc gaccaggagc acggccgtga aacggcgctt ggcccgcgcg acggccagcg
     4021 ccgcgatcac gacgacgatc gccagcggca cctgcatcac cgagtggtag agccgatggc
     4081 cggcgggcca tccgccgccc agcacggcgg ccgcaccggg cagcagcacg aacgcgacca
     4141 ggatcgtggc caggtatgcg ggcagggagc cgacctgcag gcgtcccgtc acgcccaggg
     4201 cgacgcgggc gatggccgcg atcgtgagct cgtacccgcg ctgcgcgtcg agcgggctgc
     4261 gggaggcgag cgcggtgacg cggtcgcgcc gcgcgtgcag caccatgcca ccgccgagtg
     4321 ccaccgcggt gagcaacagc ggcaggccga ggccgtgcca gagcgcgagg tggtagccgg
     4381 acacgtcggg gtacggggcg gcgtagctgg ccccgagcgc gtcgacgacc gggtaggcga
     4441 tcccgagcac gaggccggcg acggcgcaga tccagatggg cacggtgagc agcgcgcccg
     4501 gccgatggtg caccggcgtc ggctcgatgc cgggcttgcg ggcgaacgca ccccagagga
     4561 aacgggcgct gtacgccgcg gtgatcgtcg atccgagcac cagcccgagc gcgatgatcc
     4621 acccgcgcag gcccccctcg tcgaggaagc cctcgaacgc ggcctccttg cccacgaacc
     4681 cgagcagagg tggcagcccg gccatcgacg ccgcggcgag tgcggccgcg gccgccgtgc
     4741 cgggcatggc ggtgcgcagc ccggacagct tgcgcaggtc gcgcgagccc gtcgcatggt
     4801 cgatggcgcc gacggtcatg aacagcggtg ccttgaacag cccgtgcgcg agcagcagcg
     4861 ccatcccggc gagcgcggag atctctccca gcggaccgcc gcttccgagc agcacgatga
     4921 ggaacccgag ctggctgacc gtgccgaagg cgagcagctg cttcaggtcg gtggccgaca
     4981 gcgcccgcca cccgccgacg accatcgtcg cgagcccgat gacgaccacc ggcacccacc
     5041 agacggcgag gtcggagaac cccggtgcca gccgggccac caggtagacc ccggccttca
     5101 ccatcgacgc cgcgtgcagg taggcactga cgggagtcgg tgcgaccatc gcggcgggaa
     5161 gccacgggtg gaacggcagc tgcgccgact tcgtgaacgc cccgagcagg atcagcagca
     5221 gcgccgccgt gacgaccccg ccatgcagcc tgcccgcctg cccggcggcg aggatctccg
     5281 ggatcacgta ggtgcccgcg gcctccccga gcagcacgaa gccgagcagc atcgccaagc
     5341 cgccgaacac ggtgaccagc agcgcctgca ccgcggcgcg ccgctcctcc cgttgctcgc
     5401 cgccctgccc gacgagcagg aacgacgcga tggaggtcag ctcccagaac acgtagagcg
     5461 tgagcaggtc gtcggcgagc accaggccga gcatcgcgcc cgcgaaggcg agcagcaggg
     5521 ccgcgttgcg cggcgcctcc ggggagtcgg tcccgaagta gccgatgcag tacacgagga
     5581 tgacggcgcc gatccccgac accagcacaa ccatcgccag cgccagcgcg tcgagccgga
     5641 agctgagcgt caggccgagc gtgggcgccc atgccgtggt ggccgtcaga ccgccgtgga
     5701 gcgcagggcc ggtgttggcg agtgcccaga gcagcgtcgc ggcgggcagg acggccgcca
     5761 ctccgaacgc agctcgccgg tgacgcgccg acaccagcgg cagcagagcg gccaccgcga
     5821 ggtgcgccac gacgagcgtg agcaacggcg actcctcccg gtccccgggc gcgccacgac
     5881 ggccatgccc tccggacggg agagctaccc cggaacagcg tgatcatcgc ccgacctcgc
     5941 gcggcccgca ggaccggaca ccggacattt cgaacggccg ggcgctgcac ggaagcctac
     6001 cgggggtccg tttcctcccg aacctcccac cgcggacccc gacgtgaggc gcctcacgat
     6061 cgttactgga acgatccaga agatcgatgc gctataccgg atgcaggcgc atggcgcgcc
     6121 cgccgcccgc aacgcagcgg agtggcaaga accgacgagg aggacagcat gaccgacacc
     6181 accaggcgaa tcagtgccgg gctccggcgg gccctgcgtc gcctcgacga ctggaccttg
     6241 accgcgttca acccgcgcta ccccacgccg agccagcgcg actgagcgag aaccggcgag
     6301 cgccgctcct cagcggcgct cgccgggcgg taccagcggt cgcagccagg cccacagcgc
     6361 gaacagcgcg gcgaccagga tcatgacgat cccggaccac aggttgatgt cgatgccgcc
     6421 ggccttctgc agctcggccg cgctctcgaa gaacaggccc atcaccgtca ggatgatgcc
     6481 gtagacgccg aacagcagcg cgatgatcga gcgcaggtcg aacagctgcg acgcggcgct
     6541 gggagcggcg tcgctggcgc cgtggtcgac ggcggtctcg tggggttcgc tcacggatgc
     6601 ctcctgtcgg gactcactgc cgggttgtct ctgctgggtc aggcgaagag caggtacagc
     6661 acgatggtca gcgcgatcac gatcgcgccg agcacgaccg gcgactgcca ccaactgccc
     6721 tcgtcctcgc cggtgcgcat ccggtctctc ggcgtgagcg accagaccag gccgaccagc
     6781 tcggcgtcgg gcttcggcgc cgtgaccgtc gagacgacca cggcgaccgc gatcccgacg
     6841 acgaacgcag cgctcgcgcc gatgaatgac cccgcctgcg acgacacggc gatgaccccg
     6901 ctccgcacca gcgcgtcgac cgtcaccgcg gagaccgtgc cggcgatcag ccccgtccag
     6961 cccgcggtcc cggtcatccg cttccagaac aggccgagga tgaagatcgc gaacagcgga
     7021 gcgttgaaga agctgaacag ggtctggatg tagtccatca ggttctggaa actgccggcg
     7081 atgaacgccg tgccgatcgc gagcacgcag ccgacgaccg tgactgtgcg gccgacccgc
     7141 aggtagtagt cgtccggccg gccggggcgg acccagtcct gccagatgtc gtaggtgaac
     7201 accgtgttga gcgagctgac gttggccgcc atgcccgcca tgaacgaggc cagcaggccg
     7261 gccagtgcca ccccgaggat gccgttgggc agcacctcgt tcatcagcag cgacagtgcg
     7321 ttgttgtagg tgacgtcggt ggcgccgccc tgcttcagcg tggtgagctg cggcaccagc
     7381 accccggcga tcatgcccgg aacgaccacg accagcggga tcagtgcctt agggaaggct
     7441 gcgatgatag gcgtgcggcg cgcggccgac atgctgcggg ccgagagggc gcgctgcacc
     7501 tcggcgaagt tggtggtcca gtagccgaat gagagcacga acccgagccc gaagacgatg
     7561 ccgagcaccg acaggaacgg gttggcgatc tcggtgagcg cggtgcccgg ccacgacgac
     7621 acctgctccg gacctggcgg gagcgcactc accgactgct tcagcccgtc ccagcccccg
     7681 acgcgggcga gcccggcaac ggtgagcggg accagcaggg cgaggatcac gaagaactgc
     7741 aggacctcgt tgtagatcgc cgccgacagc ccaccgagga acgtgtagct gagcacgacc
     7801 agtgccgcga tcgggatcgc gaggttcaac gaccacccga gcagcgcctc gaggaccagg
     7861 ccgagtgcga acaggttcac cccggcgatc agcaccgagg ccaccgcgaa cgtcaccgcg
     7921 ttgacccgtt gggtcgtgga gttgaaccgc ttccggagga attcggggac gctacgggcc
     7981 ttactgccgt agtagaaggg catcatcacg atgccgagga acaccatcgc cggaatggcg
     8041 ccgatccagt agtagtggaa cgtggccacg ccgtactgcg cgccgttggc catcatcccg
     8101 atgagctcga tcgcgccgag gttcgccgag acgaacgcca ggcccgtgac ccaggccggc
     8161 agtgaccgcc ccgacagcag gaagtcgagg ctcgacgaga tcgaccggcg cgccatcacc
     8221 ccgatcccca gtaccaacac gaagtagaag acgacgaaca cgaagtcgat cgcggtcgtg
     8281 tcgagccgca gatcggtctg tgcgaggacc cccaccgttg ttccctccac cgctcatcga
     8341 acccgtggtg aacggatcat tcgccttgcg accggatccc gccatcgctg gcggtgggga
     8401 accatccgaa cgacacccac ttgttgccgt acccgtggcc gggtggtcac cgccgagcgg
     8461 gacgtatcgg acatccgatg tggagggtcg tcagtggacg caggcggggg gcctcacaag
     8521 caggtggtca tccgtggggc gtcgtcgacg gcagcggcaa ccagcggctt cgcgaagcag
     8581 cggctgctcg gcgagtcgcg gtagagacag aacctctgca tcggctcgta ccctgcgctg
     8641 ggatacaggg ccagcgcctc gggctgaccg gcggcgctcc ttcgtcgcgc tcgtgcgcgg
     8701 atacgagtgc cagagcactc gtatccgcgc acgggctagg aagttttggc cttctgccgc
     8761 catgtggtga gggcccgctc ggtgtagccg ttcggctccg agcggccggt gagcaccagg
     8821 tcgagggccg cctggaacga cgggctgttg tccaggtcct tcgccatcgg ctcgtagccc
     8881 ggctcgttcg cgttctgctc gtcgacgagc gccgccatac gggcgaacgt ctcccgcacc
     8941 tgcgcctcgt ccgtgaggcc gtggtgcagc cagttcgcga tgagctggct ggagatccgc
     9001 agcgtcgcgc ggtcctccat gagcccgacg ccggcgaggt cgggcaccgt ggagcacccg
     9061 atgcccagtc cgacccagcg cacgacgtag ccgaggatgg actgcgcgtt ggtctcgagc
     9121 tcgtggcgct tctcgtcgtc ggtgagctcg ccggtgagga gcggcacccg cagcagctcg
     9181 cgccggtcgg tctcgcgccc ggcgagctcg cgctgccggc cgaggacgtc ggtgcgcagg
     9241 tagtgcagcg cgtgcagggt ggcggccgtc ggcgacggca cccacgcggt gttcgcgccg
     9301 ccctccggct gcgcgccctt cgtctcgagc atctcgcgca tcgcggccgg cttggcccac
     9361 atgcccttgc cgacctgtgc ctgcccgacg aacccggcgc gcagcgccac gtcgacgttg
     9421 cggtcctcgt aggcgagcag ccatggcgtg gtcttcatgt cgttcttgcg cacgaccggc
     9481 ccggcctcga ggtccgtgtg gatctcgtcg cccgtgcggt cgaggaaccc ggtgttcacg
     9541 aagatcacgc ggtcctgggc ctgcgcgatg catgcctcga ggttgagcga cgtgcgcttc
     9601 tcctcgtcca tgatcccgat cttcagggtg gccggctcca ggccgagcgc ctcctcgacg
     9661 gcggcgaaca gctcgacggt gagggccacc tcgtcgggac cgtgctgctt gggcttgacg
     9721 atgtatacgc tgccggcgcg ggagttggag tatcgcccga gaccgcgcag gtcgtacaac
     9781 gccgcggtgg cggacaccag agcgtcgagc acaccttccg cgatcggatc gccgtcggcg
     9841 gtgcggaccg cgtcggtgcg catgtggtgc ccgacattgc gcacgagcag cacggagcgg
     9901 cccggcaacg tcagctcgga tccgtcgggg gcggtgtaac tgcggtcgcc gttgaccttg
     9961 cgctcgaccg tctcgccgcc cttctcgaac gttgaggtca gcgtgcccgt catcagcccc
    10021 agccacgtgc ggtacgcctc ggccttgtcc ggcccgtcga cggtggccac ggagtcctcg
    10081 aagtcgacga tcgtggtgac cgccgactcg agcgcgacgt cggcgacgtc ggcgtggtgc
    10141 tgacggccga cccggtgctc ggggtcgatc gtgagctcga cgtgcagccc gttgcggcgc
    10201 agcagcaccg cccgtacggc tgcggggcgc ccgtcgtcat cgccaccgcc gtcggagccg
    10261 gagcggcgcc cggcgaactg gccggggtcg gccaggccgg tgtccccgtc cttcgtctcg
    10321 acgacgagcg caccgtcctg cacccggtac gcggtagcgt ccgcatggct gcccgtcgcc
    10381 aacgggaaga gctcgtcgag cagccggtcg gcctcggcga tcacctgggc gccgcggcgc
    10441 tcgtcgtagc cgcgctcgag ctcgtggtcc agcgggagga cgtcggtgcc gtagagcgcg
    10501 tcgaagagcg agccccaccg tgcgttgacc gcattgagcg cgtaccgcgg gacggtggac
    10561 ggcaccacca gctgcgggcc ggggatctcg gcgatctcgg cgtcgacgtg ggacacacgc
    10621 acctgcggcg acgcgggcgg gagcaggtag ccgatatcgg tgaggaaggc ctcgtaagca
    10681 tcgacgaacg actcgtcgtc gccgccaccg cgctcccggt gccaggcgtc gatccgctcc
    10741 tgcagctcgt cgcgccgggc gagcagctcc tcggtgcgtc ccgcgaaccg ctcgtccagc
    10801 gccgcgcagg tggaccagaa ccactcggga tcgaggtcca ctccgggcag gagctcgtcg
    10861 gccacgaacg cacgcagcgc agggtcgacc tgcagatccg tcacgtctcc accatccgct
    10921 cgtcgttcgt ccagagtcag cgcatctgga taccccgacc tggcccgttc tagaacatcc
    10981 ttctaccagg cggactaggc attccgcatc gcggaagtga ttaggacgac accaccgcgg
    11041 cgggcgcagg gaccggagtg gccgccgcgg gcccgaccac acccgtcagc gcgagcagca
    11101 gcgccactgc tgcggcgagg aggagagaca gctgcagctt ccgacgtgga cccattgctc
    11161 ccaccttccg agggatcggc tcatggtcga tcaacgtccg ggccgtgcgg gggtgacgac
    11221 ccgcggccag agggtcgcac gcacgaccgc ccgcgccctg accgggtcgc cggcagccca
    11281 ggcgggcggg gcgtccgggg gaagccaact actgcggggt ggggcctggt ttggccggtc
    11341 accgctacga agtccaggac gaggtggcgc atgccatcgc ccatctcgca agccggcggt
    11401 cgggcctcgg ttacgggcac cgaggccgct gtggacggcg gatgtcagga ctgcgcgtcc
    11461 ggaccgcggc atgtcaggac ggcccgtccg gcccacgtga acccgctgac tgctcccggc
    11521 atgtggtacc gaggagacgt acgctcacgt cggcggagcg cagaccattc ggaccggatg
    11581 aagggggcgt cgatgccact ggccgcgggc gtgcgggcgg cgttcgagcg gatgctcgac
    11641 ccggccgacg tcatcagcaa cccggtgcag tgcagggcct acgagtgcga cggtctcacc
    11701 ggataccggg tcgtgcccga actcgtgctg ctggcgcgcg acgcggcaca ggtcgcggcg
    11761 gcggtgcggg tgtgcgccga gcaccgggtg ccgttcgtgg cccgcggtgc gggcaccgga
    11821 ctgtcgggcg gtgcactccc ggtcgcggac ggggtggtga tcagcctggc ccggctcaag
    11881 cgggtgctgg agatcgatcc gcagaaccgc cgcgccgtcg tcgagcccgg cgtcacgaac
    11941 ctggagatca ccgcggccgc ggccccgcac gggctgtact acgcacccga cccgtccagt
    12001 cagcaggtct gcacgatcgg cggcaacgtc gctgagaact ccggtggcgc ccactgcctc
    12061 aagtacgggt tcaccacgaa ccacgtgctg tcctgcgagg tcgtgctgcc cgacggctcg
    12121 atcgtcacgc tgggctccga cacgggcgag caggcgggtc ctgacctgcg cggggtgttc
    12181 ctgggctccg agggcacgct cgggatcacc acgaagatca ccgtgcgact gctgcgcacc
    12241 cccgagtcgg tgcgcacact gctcgccgac ttcccctccg tcgcggcggc gggcgacgtc
    12301 gtctcggaca tcgtggccgc agggatcgtg cccgccgccg tcgagatgat ggacaccctc
    12361 gccatcgagg ccgccgagga ggcggtgcac gcgggctaca ccgtgggcgt tccggcggcg
    12421 ctcgtcgtcg agctggacgg gccggccgcg gagtgcgacg tccagttcga gcaggtcaag
    12481 gagatctgcg accggcacgg ctgcacccgc ctgcacatcg cgggctcccc ggaggagcgt
    12541 gccgcgatct ggcgaggacg caaggccgcc ttcgccgccg tcggccggat ctcccccgac
    12601 tacttcgtgc aggacggggt ggtgccgcgc acgcggctcg ccgaggtgct cgaccggatc
    12661 gccgggatgg gccgcgacgc cggtatccgg gtggccaacg tcttccacgc cggtgacggg
    12721 aatctgcacc cgctggtgct ctacagcgcc gctgcgggag agaccgagcg cgcggagaag
    12781 ctgtcgggac agatcgccga actgtgcgtg gagctcggtg gttcgctctc gggcgagcat
    12841 ggtatcggca ccgacaaggc atgctcgatg ccgaagatgt tctcccggga cgacctggcc
    12901 gtcatgcaga gggcgcgctc tgccttcgat ccgcacggga tctgcaaccc cggcaaggtg
    12961 ctgcccacgc cgcggctgtg cggcgagcgc cccggaaagt acaagcctca tccgctggag
    13021 gagtcgggag tgatcgagcg cctgtgacac tcgagccgaa ccagaagtcc gccaccatgc
    13081 tgcggccgac cgacctacgg tccgtccgcg atgcgctgct cgacacggcc ggcaccgtct
    13141 cgatcgccgg cgccggcacg gccgccgact gggcaggttc gctcgagccc gtcgaggcgg
    13201 tgctcgacac caccggactg accggtgtga tcacgcacaa tcccggcgac atgacggtgt
    13261 ccgtgctcgc gggcactccc ctgcgcgcgc tcaccgagga gctggcgccg cacggccagc
    13321 acgtctccta cgacgcggcg cgggtcgccg acggggcgac ggtcggcggc ctgatcgcca
    13381 ccgccgacgc cggtccggcg gcgctggtct tcggatcgct gcgcgacctc gtcatcggcg
    13441 tcaccatcgt gctggccgac ggcacggtgg cccgcagcgg cggccacgtg atcaagaacg
    13501 tggccgggta cgacctggcg aagctcctgc acggttccta cggcacgctc ggcgtgctgg
    13561 tcgaggtcgt gctgcggctg caccccgtcc cgaagcgcac ggccacgctg gtggtcgact
    13621 gctcgctcgc cgacgcggcc gagcaggccg cccgcgtgct gggcggccag tacgaacccg
    13681 cggcgctgga atgggtctcg gccggggcag gcgacgggca gctgctgctg cgcgtcgagg
    13741 gcacggagag cgccctgtcc gcccggctcc aacagctgcg ggaggtgctg ggcgcgggcg
    13801 cgcaggaggc cgccgaacca gcgggcgaac ggcccgctcg tcgcgcgggt gcggacgaga
    13861 gcgccgctcg tgcggccgat ccgtgggccc accacgccac gctcgtacgg ggcacgtccg
    13921 accacgcggt actgcgcatc ggggccctgc cgagcgcact gcccgggctg ctggccgacc
    13981 tgccggccac caccgtcacc gcgggcctcg gcaccggtgt cgcgaccgtc tcgctggcgc
    14041 cggaggccgt cgcggacgcc cacgcggtcg tgcacgccgc gggcggcacg tccgtgctgc
    14101 gggcgcgtcc tgccgcgtcg gacgcgccgg catggggacc gccaccctcc gcgatcgccg
    14161 tgctgagggc cgtcaaggcg gagttcgacc cgcgcggccg gttcggcccg ggccgcttcg
    14221 ctccctggcc actgacccga aacgaggaga agggctcccg ttgaccactc ccgccggatc
    14281 cgccgagaca aatcttccgc agaccgggcc gtcgagcttc gacgaccgcc gcccgccgga
    14341 gcgggagctg ctcgacgact gcgtgcactg cggcttctgc ctgcccacct gcccgacgta
    14401 ccagctgtgg ggggaggaga tggactcccc gcgcggccgg atctacctga tggacctggc
    14461 ggcgaagggc gagatcgatc tcgacgggcc gctgaccgag cacatcgacc ggtgcctggg
    14521 ctgcatggcg tgcgtgacgg cctgcccgtc gggtgtgcag tacgaccggc tcctcgagtc
    14581 ggtgcgaccg cagatcgagc ggaacgtgga gcgcgcgccg tcggaccggc tgttccgcaa
    14641 cgcgatcttc gcgttgttcc cgtacaagcg ccggctgcgc gccgcggccg tgcctggggc
    14701 gctctaccag cggctgcgcc ggatgccggc catcgcgaag ctcgccggga agctcccggg
    14761 gcggctcgcc gcgatggagt cgctgctgcc gccggtgtcg gtgcgcgagg cgttcgcccg
    14821 gctgccggtg cacacgccgg ccgtcggcga gcggcgggcg cgcgtcgcct tgctgtcggg
    14881 gtgcgtacag gacgtgttct tccaccgggt caacgaggcc accgtccgtg tgctcgcggc
    14941 cgagggatgc gacgtgctgg tgccggccga ccagcagtgc tgcggcgcgc tggagctgca
    15001 ctcgggccgt gaggacagcg cgctggtcag ggcgaggcgt gcgatcgcgg tcttcgagac
    15061 cctggacgtg gaccacgtcg tcaccaacgt cgccggctgc ggctcgtcga tgaaggagta
    15121 cggccacctg ctctccgacg acccggagtg ggccgagcgg gcggcggcgt tcagcgcgcg
    15181 cgtgcgtgac gtgcacgagg tgctcgccga actgggcccg cgcgcgccgc ggcacccgtt
    15241 ccgcggtcga gttgcctacc acgacgcctg ccacctcggg cacgcgcaga aggtgcgtgc
    15301 tcagccgcgc gcggtgctgc gctcgatccc ggacctcgag ctggtggacc tgccggaggc
    15361 cgagctgtgc tgcggctccg ccggcatcta caacatgatc atgccggacg ccgccgctga
    15421 tctcggcgcc cgcaaggcgg ccaacgtgcg ctcggtggag cccgacgtga tcgtcaccgc
    15481 gaaccccggt tgcctgttgc agatcggcaa gtacctcggg gccgccggcg gcgcggcgga
    15541 cggcggcggt ccgatccccc tcctgcaccc cgtgcagctc ctggacgcgg cgatccgcgg
    15601 ggtgccggta ccgcgacgct gaccgaccac gagcggagac cggacgagcg gcgcactcgc
    15661 gcggaccgaa cggaccacgg tgccctttgt gcgggtgccg cggcggggcg tcgcggcttc
    15721 tgtcggggtt cgcccatacc gtctgccgtc atgagcatcg actggacacg catgctcgtc
    15781 gagcaactgg agtggcactg gcaggagcac ctgcgcccgc ggctcgacgg actcgccgac
    15841 gacgagtact tctgggagcc ggcgccgggg gcgtggaacg tccgcccgcg cggcacgtcc
    15901 accgcgcccg tacaggccgg ctgcggtgag ttcaccgtcg acttcgcgtt tcccgagccg
    15961 gttccaccgc cggtcaccac gattgcctgg cggctggccc acctgatcgt cggggtgttc
    16021 ggggcccggg cgggatcgca tttcggcggc aggccgatcg actacgagag cttcgattac
    16081 gccggttcag ccggagccgc cctgcgacag ctcgacgagg tgcaccacgc ctgggtggcg
    16141 ggcgtacgcg ggttggatgc cgccgccctg gagcgcccgg tcgggtcggc ggagggtccc
    16201 ttcgccgagc accccatggc cgagctcgtc ctgcacatca accgcgaggc gatccaccac
    16261 ggcgccgagg tcctcctgct gcgcgacctg taccggacca cagggacata acggtcacca
    16321 gaccgcggcg aattaggctt aatgggctat ctcgctgtgc cggatcaaga gcggcaccgc
    16381 acactgcgcg accatgtggg ccactgtcga tctgctgatc ttgtcctccg cacatccacc
    16441 ggccggcgtc gtgctggccg cgtacgagca gaatccggag gccgtcggcg ggtctctcgg
    16501 gctctccgcg ctggtctcgg tgataccgct ggccgtcgtg ctggtgctgc tcggtgtcct
    16561 gcgggtccgc gcgccgtggg cggcgctggc cgggctcgcc gccgcgctgg tggtggcgat
    16621 catcggcttc gacatgccgg tggggatatc cctgtcggcg gccgcgcacg gggccactta
    16681 cggcctcttc ccgatcctgt ggatcgtggt gaacgccctc tgggtgttca acatgaccgt
    16741 ggccaccggc cacttcgacg tgctgcgccg cagcttcggc gcgctgtcga tcgaccagcg
    16801 cacgcaggcg atcctggtgg cgttctgctt cggcgggctg ctggaggccc tcgccgggtt
    16861 cggtgccccg gtcgcgatca ccgcggtgat gctcgtggca ctcggattcc agccgctcaa
    16921 ggcggcggtg gtcgccctgg tggccaacac cgcccccgtc gcgttcggag cgatgggcgt
    16981 tccggtgatc acgctcgccc gcgtcaccgg gctgccgctg gaccaggtgg cgtccatggt
    17041 cggacggcag acgccgctgc tcgcgttgtt cgtcccactc gtgctggtgt tcatcgtcga
    17101 cggcaggcgc ggcgtgcggg aggcctggct gccggccgtc gcgtgcggtg tcgtgttcgc
    17161 gatcggacag ttcctgacgt cgaacttctt ctccgtagag ctcaccgaca tcgtcgcgtc
    17221 gctgctcggc gcgatcgtcg tgttcgtgct ggcgcgggtc cgcccgcagg cggtgtgggc
    17281 ggcccggcag tcgacgatgg ccgcggcagg tggtcccggc acagcggcga agcccgactc
    17341 gcccaccgag gtgcgcaagg cctatgcgcc ctacgtgatc atcgtggtcg tcttcgcgct
    17401 ggcccagctc cccggcctca agccgctgat cgactccctg aagatcgcct tcaagtggcc
    17461 gttcctcgac gtcgtcggta tcgacggcaa gccgcacagc accaccatgt tctcgctgac
    17521 ctggctgtcc tcggcaggca ccctgatggt gatcgccggc gtcatcgtgg cggcccttct
    17581 cggcgtgggg gcacggcgtg ctctcgccac ctgggggcgc accgtggtcg acctgcgctg
    17641 ggccatcctc accgtggccg cggtgctggc gctggcctac gtcatgaacg cctcggcgca
    17701 gaccacggcg atcgggctgt tcgtggcggg tgccggcggc gcgttggcct tcctgtcccc
    17761 ctggctcggc tggctcggtg tggcagtcac gggttcggac acctccgcca acgcgctgtt
    17821 cggctcgctg caggtcaccg ccgcccaggg cgccgggctg ccgcccgaac tgctggccgg
    17881 cgccaactcc tcgggcggtg tgctcggcaa gatgctctcg ccgcagaacc tcacgatcgc
    17941 cgcggccgcc gtgaagctgg agggccagga gggcgtgctg ctgcgcaagg tgatcggctg
    18001 gagcctcggg atgctggtcg tgctttcggt gctcgtcgcg ctgcaggcca ccccggtgct
    18061 cggctggatg cttccgtagc tgctcggcca gcccccggga gccgaactgc acgcgcggat
    18121 cccaggggtc tgccgccata ccggtcgctg cgctcagcgg gcgaagtggt cccagccggc
    18181 ggtgccgctc tcgtagggcc tgccgtcgac cagaacccgg ggatcacccg ccctgaccgt
    18241 gccgatcgcc gtccagccgt cggggaccgg ccccgggaac gtcgccgcga gcgcgtggtc
    18301 ctcgcccccc gtgagcagcc actccagcgg gtcgacgccg agtgcggcgc cgacgtcgac
    18361 cagccgggcg ggcacctcga aagccttgct gcgcacgtcg agcacaacac ccgacgcccg
    18421 cgcgacgtgc ccgaggtcgg cgacgagacc gtccgagatg tcgatcatgg acgtggcccc
    18481 ggccagcgct gcctgcggcc cggcggcgta gggcggttcc ggcacgcggt gcgcgccgac
    18541 caccgccacc ggggagcgga acccgcgaga cagtactgcc agccccgccg ccgcccaccc
    18601 gatccgcccg cacagggcga gcgcctcgcc cggccgcgcc cccgcccggg tgaccggcga
    18661 cctgccctca agcgatccca gcgctgtcac cgagagcacg agagtcgggg atgccgccac
    18721 gtcaccgccg acgaccccga cgcccgcgag gtccgcctcc gcccacagtc cgtcggccag
    18781 gccggtcagt gtctcgaccg gggtgtcctt gctgcaggcg agcccgacga gcagcgccgt
    18841 gggcaccgct cccatcgccg cgacgtccgc caggttggcg gccgccgcct tgcgccccac
    18901 ctggtcggca gccgaccagt cgagccggaa gtgcacgccc tcgaccagca cgtcggtgca
    18961 ggcgaccacg cggccgtcgg gcgcggccac caccgcggcg tcgtcccctg ggccgagaag
    19021 cgtgccggcg ggctgcgtgc gcccccgggt cacccggctg atcagaccga actcaccgat
    19081 ggcagccagt gtgttctccg cctcggcgtc cccggtcggg ggcgggaacg atgtcggcac
    19141 agctgccacc tccggtacgt tcggcttccg actttcctac cccggagagc tcgaccgaag
    19201 tggaaggggc acgccgtggt gcaggcttac atcctggtcc agaccgaggt cggcaaggcc
    19261 gccgccgtcg ccgccgagat ctccggcatc accggcgtgg tctccgcgga agacgttacg
    19321 ggtccctacg atgtgatcgt gcgcgctgag gccgacgacg tcgatctgct cggccagctc
    19381 gtggtcgcgc aggtccaggg cgtgggtggc atcacgcgga ccctcacctg cccggtggtc
    19441 cacctctcct gaccatggca ccaccacccg tccgctcgag gccgtcgctg ccggtcgtga
    19501 tcgcgatcgc gatcccgctg atcttcgccg tggtggtggc cgcgatctcc atcacaatgc
    19561 ggggtcgcgg ggcggccgaa cccccacgcc cggacaccgg cccactcgcg gtcgtgcccg
    19621 tcgacgcacc cgatgcggcg gggccggagt gcacgatcct gctcgccgcg ctccccgccg
    19681 acctgtccgg agcgggcggc actctgcacg aacggccgct ggccgacccg gccccgcccg
    19741 gggtgcgggc ctgggcggcg gcgccccaac cggtcgtgct gcgctgcgga ctcccgcgcc
    19801 cggtcgagct gaccccgacc gcagccctcc tcgaggtcaa cggggtgcgc tggctgaagc
    19861 tcgacgacgg ggtacccgac ccggatgtgg cgagctacgt ggccgtggac cgtccggtct
    19921 acgtggtgct caccgcaccg ttggacgccg gcagcgggcc gctccagcag gtgtccgacc
    19981 tcgtgcgcaa ttcccttcct gccaccgagg tgaaggtgcg ctagcagaat ctgttagaca
    20041 catctaacat cgcttttcat ggtgactatg tcccgggcga ccgaggacta cctcaaggcg
    20101 atctaccacc tcgcccaccg aggagagccg gtcaccacgg gcctgctggc ccacgagctc
    20161 ggcgtctcct cgccgtcggt ctcgtccatg gtcaagcggc tggaggacgg ggacctgctc
    20221 acccgcccgg atcaccggtc gctgcacctc accgacagcg gggaacgggc ggcactgcgg
    20281 gtggtgcgcc gacaccggct gctggagacg ttcctcgccc gcatgctcga cgtgccgtgg
    20341 gacgaggtgc acgccgaggc cgaactgctg gagcacgccc tgtccgaccg gctcgaggag
    20401 cgcatcgacg ccgttctcgg ccaccccacg cacgacccgc acggtgaccc catccccccg
    20461 cgcgacggcc cgcacgagga gagctggggc gaggcgctgg ccaccgtgcc gcccggcagc
    20521 cggttcctgg tggagcgcgt ctcggatcgc gacagcgggg ctctgcggta tctcggcgac
    20581 ctcggagtgt ttcccggggt cgtcgtcacc gtcgaggagc aggcgccgtt cggcgggccg
    20641 cggtgggtgc gaatcggtgg cgagcggcac ccgctgggcg ggccactcac gcagctggtg
    20701 cacggccacg tcgtcggcga ggcccacgtc gtcgagaagg ccgaacagtg acggccacga
    20761 agggcaccgc gccgagcgtc gacgagatca gggcacgggg cgggctgcgg agccggctcg
    20821 cgttcttcgg cccggcgatc gtcgccgcga tcgcctacgt ggaccccggc aacttcgcca
    20881 cgaacttctc ggcgggtgcc gggttcggct acctgctgct gtgggtgatt gtcgccgcga
    20941 acctgctcgc gatgctcatc cagacgctgt cggcgaagct cgggctggcc accgggcgca
    21001 acctcccgga gatgtgccgc gagcagttcc cgcgcccggt gacgcgcgtg atgtgggtgc
    21061 aggccgagct cgtggccatc gccaccgacc tggcggaggt gatcggcggc gccatcgcgc
    21121 tgaacctgct gttcgggatc ccgctgctca ccggcggagt gatcaccgga atcgtcgcgt
    21181 tcgcactgct cggcctgcag gccaggggct accgcccgtt cgagctggcc atcgcgggcc
    21241 tgttcggcgt catcctgctc gggttcctca gcacggtgct acgtatcggc atcgacgtgg
    21301 gcgacgccgc tgccgggctc gtcccccgct tcgagggcac cgacagcctg ctgctggcca
    21361 ccggaatcct cggcgccacc gtcatgccgc acgtcatcta cctgcactcc gcgctcacga
    21421 acgcccggat ccccgccact tccgtcagcg accgccggtt cctgctccgc taccagcaga
    21481 tcgacgtcct gatcgggatg gggctggccg gcctggtcaa cgcgtccatg ctgctgatct
    21541 ccgcggcgtt gttcttcggc accgacgtgc cggccaccga caccctcggg ggggtctacg
    21601 cggggctggc gcgagtgctc gaccagcccg cggcgttcgc gttcgcactg gccctgctgg
    21661 cctcgggctt cgcctcgtcc ggggtgggca cctacgccgg gcaggtcgtg atgcagggtt
    21721 tcatccagcg ccggataccg ctgttgctgc gcagactcct caccatcagc cccgcgctcg
    21781 tcgtgctggg tatcggctgg gaccccacca gagcgctcgt gctctcccag gtggtgctgt
    21841 cgttcggcat cccgttcgcg ctggtgcccc tggtactgct cacccgccgc cgcgacgtga
    21901 tggccgagct cgtcaaccgc aggctcacca ccgcggtcgc ggcgctggct gcggcggtga
    21961 tcatcgcgct caacgccttc ctgatctacc ggaccgtcac cgggggctga ggggcggcgc
    22021 aggtcttcaa cgcaggccgg tgccgcgcgc gatggccgtc tccacgagtg tcgtgagcag
    22081 ggtcgggtag tcgatccccg tcactgccca ggcgcgcggg aacatcgaga tcggggtgaa
    22141 gccgggcatc gtgttgacct cgttgatggt cagctccccc gcgggcccga ggaagaagtc
    22201 gacgcgcgcc agaccttgac cgtcgagcgc ccggaacgct gccacggcca tctcccgcag
    22261 ccggtcggac accgcgtcgt ccaacttggc cgggatgtcg aactcgcaga cgtcatcgag
    22321 gtacttggcc tggaagtcgt agaactcagg tgtgctaccg ccggcgccgg tgccgacgat
    22381 gcggatctcg gcgggcacgc tcgcccgcac cgtcccgtcc gggaactcga gcacgccgca
    22441 ctccacctcg cggccgacga ttgcggcctc caccagcacc ttcgggtcgt gctctcgggc
    22501 cagggcgatc gcgccgtcga gtacggccca atcggtgacc cgggtgatcc ccaccgacga
    22561 cccagcccgc gcgggcttca cgaacaccgg cagcccgagc cggtcacgct gctcggacgt
    22621 gagcgtggcg gtgcccggac ggagcaccac gaggtctccg acgggcaggc cctcggccgc
    22681 cagcagcttc ttggcgaact ccttgtccat cccggctgcg ctcgcgagga ttcccgcccc
    22741 gacgtacggc acgcccgcca tctccagcag gccttggatc gtgccgtcct cgccgaaggg
    22801 gccgtgcagc accgggaaga ccacctcgac gcgggagaag acctcgcccg cccgatcggg
    22861 atcgaggacg acgagcccgc cccgcgtcgg gtcgccgggc agggccagcg ccgtgcgcga
    22921 ctcgtcgacg gacgggagca cgcgatcgga gatcgccagc tcgcgcgggt cgtcggtgcc
    22981 gagcacccag gccccggacg tggtgatgcc gaccggcacg acctcgaagc gctcccggtc
    23041 gaggtacgag agcaccgacc cggcggagat gcaggagatc gcgtgctcgc tgctgcgccc
    23101 gccgaacacc acggccaccc gcaccttgtc cccgctcatg gcgcccgaga gtaggcggag
    23161 tgggccggcc gggcagacgg gggctcctgc gagccaggcg gaattcccac tcagctatag
    23221 cggggagccc gtcgcacggc tcgtcccggt gcagcgccgg gcgccgcgca cctcggacgt
    23281 gacgcaggcc tgttcgaggc gcccgaggac ttcgac
//
LOCUS       DASRSC010000322         6258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5047334,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000322 DASRSC010000000
VERSION     DASRSC010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6258
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5047334"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(591..1919)
                     /locus_tag="VFE39_15825"
     CDS             complement(591..1919)
                     /locus_tag="VFE39_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET6260105.1"
                     /translation="MQENDRTRPLPPDDSYLRSLTVATERAVRDAEPIGSAHDGAPGD
                     VRGLVRTAAGGLDDELVALSHDLHAHPEEAFAEHRSVRAVADLLERHGHGAEIGIGGL
                     ATSLVATAGTRGPHIAVLAEYDALPGVGHACGHNIICATATGAFLAAAQVQDRVGGRV
                     SLIGTPAEEGGGGKETMARAGVFADVDAVIMLHPFTHDIAMHPFLGRRQVEMVFRGVA
                     AHAAAQPFMGRNALDAAVAAYQGVAALRQHLPGSDRVHGIFSDGGARPNVVPERAALL
                     FYLRSAAPETLRDLAGRVDSIAHGAAAMTGCTVELRWDELPPYLPIRFNQALAGRWAV
                     NQEPVGRTPLPTGVVPEFLTGSTDLGNLSYRMPAIHPMIAVSEPTVALHTREFATASG
                     GPAGDRAVRDGALGLALTAVDYLADAGLREAVHAEFAAAGGALDVPTYFH"
     gene            complement(2070..2726)
                     /locus_tag="VFE39_15830"
     CDS             complement(2070..2726)
                     /locus_tag="VFE39_15830"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HET6260106.1"
                     /translation="MRIVDLSQPVHAGMQVYPGDPEVAVTPAATLDRDGVNVLAVHSG
                     SHTGTHVDAPFHVLADGARLDELDLALFHGPAVTADLLGRAPRTAVRWADLEPVADRL
                     RAGVVLVLHTGWSVHFGTPAYLEHPWLTPDAAERLVATGVRALAVDTLNPDPTPPLGT
                     GIGDLPVHRAVLGAGGVIVENLTNVAQLATLSDPLLSFFPLHLAGADGAPVRAVGLGT
                     "
     gene            complement(2731..3312)
                     /locus_tag="VFE39_15835"
     CDS             complement(2731..3312)
                     /locus_tag="VFE39_15835"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HET6260107.1"
                     /translation="MRDSRMGIFVGWYSKITPRRWVVAGRAAILVGAVGAGVLGMAVG
                     PLTGLATANAATPTPSRTAPSGTAPAATTSLVPGTPCTAIAKACIDLAHNEAWLVNDG
                     HVVRGPVRISPGAAGENTPIGTFDVQWKDLHHRSKEFNNAPMEYSVFFAPGGIAFHEG
                     SLQKRSAGCVHLSAVNAAAWYSYLKVGDQVQVH"
     gene            complement(3482..>6258)
                     /gene="hrpA"
                     /locus_tag="VFE39_15840"
     CDS             complement(3482..>6258)
                     /gene="hrpA"
                     /locus_tag="VFE39_15840"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HET6260108.1"
                     /translation="KVQRLPIEKVSQASANQRAGRCGRVADGVCIRLYSEDDFDARPG
                     FTDPEILRTNLASVVLQMIALELGALETDRRLTPVGRALAALPVDPRLGRMLVEADRT
                     GCLREVLVIAAALSVQDPRERPTEQRQAADQKHARFTGAWTAGGTTLDADGSDFIAFL
                     NLWRYLAEQRDALSGNRFRRQLREEFLHYLRIREWQDLHSQLRSAARNAGMTLNDTEA
                     DADRIHVAVLSGLLSQIGLRDGGAETAGAARGRADAASAPRGGGNREYLGARGAKFMI
                     FPGSSLAKKQPRWVMAAELVETSRLFARTVARIKPEWIEPLAGHLIKRSYSEPHWSRK
                     RAAAVATERVTLHGIPVVVGRTVDFGRIDPEESRELFIRHALVEGDWDTRHRFFHANR
                     ELLEDVEELEHRARRRDLVVDEETLVAFYTDRIPADVVSGRHFDSWWKKTSRTRPDLL
                     DFTEEMLRTAQADAVDRSAYPDHVEAGGLTLPLSYAFEPGARTDGVTVDVPVAALHQV
                     DPTPFTWQVPGLREELVTALIRTLPKQLRRLYAPAPDHARAVLARLHSADAPDEPLLD
                     GLERELGRMRGVDIPRAAWELDRLPEHLTVTFRVVDETGREVATGTDLDALRRRLAPK
                     VREELAAAGAGIERTGLTTWTLDTLPRELPVRRGGHVVTGHPGLVDRGATVDVRVFPT
                     AAERDPQMWRGVRRLLLLDTPSPAKQVQRGLDNASKLALSRNPHGSVAALLDDCVTAA
                     ADALITAAGGPPWDERSYVRLRKLFAERLASTTLQVLQAVRGVLTIWHRVQAQLGELR
                     APVLAAGVADLTAQVNALVGPGFATTAGPSRLADVARYLEAVERRIEKLRADPVRDAG
                     WTAQVQVVADEYAAELAGLPPGVEPSPQLREIRWLIEELRVGLYAHPMRTRYPVSIKR
                     IQKAIDDLPR"
BASE COUNT          859 a         2213 c         2311 g          875 t
ORIGIN      
        1 cttcgagcgc gccgcggagc tgttcgcgga gggcgcgctg cggccggagg tgatgatcag
       61 ccaccggatg ggcctcgacg agtacccgca ggcgctcgag cagttccgct ctggcgtggg
      121 gcgcaaggtc caggtcctgc ccggtaagcg ctgaccgggg gcggcagctc ccgtcgtacc
      181 ggcctgaccc gctctcgctc gtggacgcgc agaccgcggt cgccgatgtc cggcgcttgg
      241 tggaggggct gcgtccgccg accctggacg cgatgggcct ggtcggcgcg gtccgagccc
      301 acctcgcggg gcccgccgtc gcgctcgacg cgcccgacga ccttcccgcg ctgggagcgg
      361 ggggaagcgg tcgcagccgt cgcggcctcg gcagcccaac gtggtcctgc tcgacctggt
      421 cgcccgcaac ctcaccaacc cgcagatcgc ggcggagctc gggctgagcg gcaagaccgt
      481 ccacaaccac gtgtcgaact cttcacgaag ctgcaggtcg ccgaccgcgc ccaggccact
      541 gcgatggctc ggcgctccgg gctgggagcg gcaggcgagg agcgttcggt tcagtggaag
      601 taggtgggga cgtcgagggc cccgcccgcc gcggcgaact ccgcgtgcac ggcctcccgc
      661 agcccggcgt cggccaggta gtcgacggcc gtgagggcca gcccgagggc gccgtcgcgc
      721 acggctcggt ccccggccgg tccgccggac gccgtagcga actccctggt gtgcagcgcc
      781 accgtcggct cggacaccgc gatcatcggg tggatggccg gcatgcggta gctgaggttg
      841 cccaggtcgg tggagccggt gaggaactcg ggcaccacac ccgtcggcag cggcgtgcgc
      901 ccgaccggtt cctggttgac cgcccagcgc ccggccagtg cctggttgaa gcggatcggc
      961 aggtacggcg ggagctcgtc ccaccgcagt tccaccgtgc acccggtcat ggccgccgca
     1021 ccgtgggcga tggagtcgac gcgtccggcc aggtcgcgca gggtctccgg cgccgccgaa
     1081 cggaggtaga acagcaacgc cgcccgctcg ggcacgacgt tgggccgggc gccgccatcg
     1141 ctgaagatcc cgtgcacccg gtcgctgccc ggcaggtgct gacggagcgc ggcgacgccc
     1201 tggtacgcgg cgaccgcggc gtcgagtgcg ttgcgaccca tgaacggttg cgcggccgcg
     1261 tgcgcggcga ccccgcggaa caccatctcg acctgacggc ggccgaggaa cgggtgcatc
     1321 gcgatgtcat gcgtgaacgg gtgcagcatg atcacggcgt cgacgtcggc gaagacgccc
     1381 gcccgcgcca tcgtctcctt gcccccgcca ccctcctcgg cgggcgtgcc gatcagcgag
     1441 acccggccgc ccacccggtc ctgcacctgc gcagcggcga ggaacgcgcc ggtggccgtt
     1501 gcgcagatga tgttgtggcc gcaggcgtgc ccgacgccgg gcagcgcgtc gtattcggcg
     1561 agcaccgcga tgtgcgggcc gcgcgtgcct gcggtcgcga cgagcgatgt cgccagccct
     1621 ccgatgccga tctccgcgcc gtgcccgtgc cgctccagca ggtccgccac cgcacgcacc
     1681 gaccggtgct cggcgaacgc ctcctccggg tgcgcgtgca ggtcgtggct gagcgcgacg
     1741 agctcgtcgt caaggccgcc cgcggcagtt ctgacgagcc cgcgcacgtc gccgggcgcg
     1801 ccatcgtggg cggagccgat cggctctgcg tcccgcaccg cccgctccgt cgcgaccgtg
     1861 agcgagcgga ggtagctgtc gtcgggcggc agcggtcggg tccggtcgtt ctcctgcatg
     1921 atcgcgacag tagtgcggcg accacgaact ttcaccgcgt cctcgcgggc ccagatgacg
     1981 cctcgcggcg ttgccgccct tgcgaagaca cccggtattg ctccggcaac ggttgtggcc
     2041 accgcactgg gcgaaccggg gctcccgggt caggttccca ggccgaccgc acgcaccggg
     2101 gcgccgtcgg cgccggcgag gtggagcggg aagaagctga gcagcgggtc ggagagggtg
     2161 gcgagctgcg cgacgttcgt gaggttctcc acgatcaccc cgcccgcgcc gagcaccgcg
     2221 cggtgcaccg gcaggtcgcc gatgccggtg ccgagcgggg gcgtcgggtc cgggttgagg
     2281 gtgtccacgg ccagcgcgcg cacgccggtt gcgacgagcc gctcggccgc gtccggcgtc
     2341 agccacgggt gctccaggta ggcgggcgtg ccgaagtgca ccgaccagcc ggtgtgcagg
     2401 accagtacga cgccggcccg gaggcggtcg gcgaccggct cgaggtccgc ccagcgcacg
     2461 gccgtacgcg gggcgcggcc gagcaggtcg gccgtcaccg ccgggccgtg gaagagggcg
     2521 agatcgagct cgtccagccg ggcgccgtcg gcgagcacgt ggaagggcgc gtcgacgtgc
     2581 gtgcccgtgt gcgacccgga atgcacggcc agcacgttga ccccgtcccg gtcgagggtg
     2641 gcggcaggtg tcaccgcgac ctcgggatcg ccgggataga cctgcatccc cgcgtgcacg
     2701 ggctgggaga ggtcgacgat tcgcatcctg tcagtgcacc tggacctggt cgccgacctt
     2761 cagatagctg taccaggccg cagcattgac ggccgacagg tgcacacagc cggcggacct
     2821 cttctgcagg ctgccctcat ggaaggcgat cccgccgggg gcgaagaaaa cggaatactc
     2881 catcggcgcg ttgttgaact ccttgctgcg gtggtgcagg tccttccact gcacatcgaa
     2941 ggtgccgatg ggggtgttct cacccgcggc gcccgggctg atccgcaccg ggccgcgcac
     3001 gacgtgtccg tcgttgacga gccaggcctc gttgtgggcg agatcgatgc acgccttcgc
     3061 gatcgcggtg cacggggtgc cgggcaccag ggaggtggtg gcggccggag ccgtgccgct
     3121 gggggctgtt cgggagggag tgggggtagc cgcgttggcg gtggccagtc cggtcagcgg
     3181 cccgacggcc atccccagca cccctgcgcc gactgctccg acgagtatcg ccgcccgacc
     3241 tgcaacgacc cagcgccggg gtgtgatctt gctgtaccaa cccacgaaaa tacccattct
     3301 gctgtcccgc acgacctacg atactgatcg atcataaagg caacgaacgt atcggccgcg
     3361 tgatgctgcc cggctgtctc ggcctttcgg atgtcgctgt accgacattc cggtacgtgg
     3421 aggtatggcc ctttttgtga ggatgagact cgcacaatga cacggtcccg gggtgggaga
     3481 gtcagcgggg caggtcgtcg atcgccttct ggatgcgctt gatcgacacc ggataccggg
     3541 tgcgcatggg gtgggcgtac agccccaccc gcagctcctc gatcagccag cggatctcgc
     3601 gcagctgcgg cgacggttcc acccccggcg ggagccctgc aagctcggcc gcgtactcgt
     3661 cggccaccac ctgcacctgc gcggtccagc cggcgtcgcg gacggggtcg gcccgcagct
     3721 tctcgatgcg ccgctccacg gcttccaggt agcgggcgac atcggcgagc cgggacggtc
     3781 cggccgtggt ggcgaaaccg gggccgacca gggcgttgac ctgtgccgtc aggtcggcga
     3841 cgcccgccgc aagcaccggc gcgcgcagct cgccgagctg tgcctgcaca cgatgccaga
     3901 tggtgagcac accgcgcacg gcctgtagta cctgcaacgt cgtggaggcc agccgctcgg
     3961 cgaacagctt ccgcagccgc acgtaggacc gctcgtccca cggcgggccg ccggcggcgg
     4021 tgatcagtgc atcggcggcg gcggtgacac agtcgtcgag cagcgcggcg acggagccgt
     4081 gcgggttacg ggacagcgcc agcttcgacg cgttgtccaa gccgcgttgc acctgcttcg
     4141 cgggcgaggg cgtgtccagc aggagcaggc ggcgcacgcc gcgccacatc tgcggatcgc
     4201 gctcggccgc cgttgggaac acacggacat cgacggtggc accgcggtcg accagtccgg
     4261 ggtggcccgt gacgacgtgg ccgccccgac gcaccgggag ctcgcggggc agcgtgtcga
     4321 gggtccacgt ggtcagtccg gtgcgctcga tcccggcacc tgccgcggcc agctcctcgc
     4381 gcaccttcgg ggcgagcctg cgccggagcg cgtccaggtc ggtgccggtc gccacctcgc
     4441 ggccggtctc gtcgaccacc cggaacgtga cggtgagatg ctcgggtagc cggtccagct
     4501 cccaggcggc gcgagggatg tcgacgccgc gcatgcggcc gagctcgcgc tccaggccgt
     4561 cgagcagcgg ttcgtccggt gcgtcggcgg agtgcagcct cgccagcacg gcccgcgcgt
     4621 ggtcgggtgc gggagcgtaa agccgccgca gctgcttggg cagcgtccgg atcagcgccg
     4681 tcaccagctc ctcgcgcagg cctgggacct gccacgtgaa cggggtcggg tccacctggt
     4741 gcagtgcggc caccggcacg tcgaccgtga cgccgtcggt acgtgcgccc ggctcgaacg
     4801 cgtacgacag cggcagcgtg agccctccgg cctcgacgtg gtcggggtag gcggagcggt
     4861 ccaccgcatc ggcctgtgcc gtgcgcagca tctcctcggt gaagtcgagc aggtccggac
     4921 gggtccgcga cgtcttcttc caccacgagt cgaaatgccg cccggacacg acatcagccg
     4981 ggatgcgatc ggtgtagaac gcgacgagcg tttcctcgtc gacgaccagg tcgcggcggc
     5041 gcgcccggtg ctccagctcc tcgacgtcct cgagcagctc ccggttggcg tggaagaacc
     5101 ggtgccgcgt gtcccagtcg ccctcgacga gcgcgtgccg gatgaacagc tcgcgcgact
     5161 cctcgggatc gatccggccg aagtcgaccg tgcgcccgac gacgaccggg atcccgtgca
     5221 gcgtgacccg ctcggtggcg acggccgcag cccgcttgcg cgaccagtgc ggctcggagt
     5281 acgagcgctt gatcaggtgc ccggccagcg gctcgatcca ctccggcttg atccgtgcga
     5341 ccgtgcgggc gaacagccgc gaggtctcca cgagctcggc tgccatcacc cagcgcggct
     5401 gcttcttcgc gagcgacgag cccgggaaga tcatgaattt ggcaccgcgg gcaccgaggt
     5461 actcgcggtt tccgccaccg cgcggagcgc tcgcggcatc ggcacggccg cgcgcagccc
     5521 ccgccgtctc ggcaccgccg tcgcgcagcc cgatctggga gagcagcccg gacagcacgg
     5581 cgacgtggat ccggtcggcg tcggcctcgg tgtcgttgag ggtcatgccc gcgttgcggg
     5641 ccgccgaacg gagctggctg tgcaggtcct gccactcacg aatgcgcagg tagtggagga
     5701 actcctcccg cagctgccgg cggaagcggt tgccgctgag cgcgtcgcgc tgctcggcga
     5761 ggtagcgcca gaggttcagg aaggcgatga agtcggaacc gtcggcgtcg agcgtcgtcc
     5821 cgcctgcggt ccacgcgccg gtgaaccgcg cgtgtttctg gtccgcggcc tgacgctgct
     5881 cggtcgggcg ctctcggggg tcctggaccg acagcgccgc ggcgatcacc agcacctcgc
     5941 gcaggcagcc ggtgcggtcg gcctcgacca gcatgcggcc cagccggggg tcgacgggca
     6001 gcgccgccag cgcccggccg acgggagtga gcctccggtc ggtctcgagg gcacccagct
     6061 ccagcgcgat catctgcagc acgaccgacg cgaggttggt gcgcaggatc tccgggtcgg
     6121 tgaacccggg acgggcgtcg aagtcgtcct cgctgtagag gcggatgcag acgccgtcgg
     6181 cgacccggcc gcagcggccc gcgcgctggt tggcgctcgc ctgcgacacc ttctcgatgg
     6241 gcagccgctg caccttca
//
LOCUS       DASRSC010000323         4618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1446904,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000323 DASRSC010000000
VERSION     DASRSC010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1446904"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /locus_tag="VFE39_15845"
     CDS             <1..967
                     /locus_tag="VFE39_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsH"
                     /protein_id="HET6260109.1"
                     /translation="EELVFHEPTTGASSDIDQATKIAKAMVTEYGMSARLGAVRYGRD
                     QGDPFLGRSMGNQADYSLEVAHEIDEEVRALIEAAHTESWEILNTYRDVLDDLVFELL
                     EKETLTRRDLERIFDRVEKRPRITAFNDFGGRTPSDKPPIQTPAERARERGEPWPPHT
                     EPARDPAPVGSYPGGGYAVPAPGPPNGGPVPTPGAPGYPGGPGQPPFPTAAPGGYGPS
                     APQQTWGSPPNPGQGQQPNPGQPNSVQPNYGAPADWRPATTPHGQSWPPAGQWQQQPQ
                     QPQQWPPQQWPPVAGNGHGGDGSAAAPSPEPPHENGKAPNEDGSR"
     gene            1045..1608
                     /gene="folE"
                     /locus_tag="VFE39_15850"
     CDS             1045..1608
                     /gene="folE"
                     /locus_tag="VFE39_15850"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET6260110.1"
                     /translation="MDHARAEAAVRELLIAIGEDPERDGLRDTPARVARAYEEMFAGL
                     YTEPDTVLERTFDEHHQELILVKDIPIFSTCEHHLVPFHGMAHVGYIPNKDGRVTGLS
                     KIARLVDLYARRPQVQERLTGQVADALVRKLAPRGVIVVIEAEHLCMGMRGIRKPGSR
                     TVTSAVRGMLQSSPTSRAEALALIRSG"
     gene            1668..2498
                     /gene="folP"
                     /locus_tag="VFE39_15855"
     CDS             1668..2498
                     /gene="folP"
                     /locus_tag="VFE39_15855"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET6260111.1"
                     /translation="MGVLNVTPDSFSDGGRYLDREHAVAHGVAMHEAGADLVDVGGES
                     TRPGAGRVEAGVEKARVLPVIRELVAEGVQVSIDTTRAAVAEAALEAGASIVNDVSGG
                     LADRAMPATVAQARVPWILMHWRGHSDRMNALATYEDVLGEVRDELVSRVDAAVLAGV
                     EPSRLVLDPGLGFAKTAAHNWTLLRRLDVLTSLGFPVLVGASRKRFLGQLLAGADGSL
                     RPFDYRDGATAAISALAAYNGAWGVRVHDVHGTLDAVAVATAWQLGASKARTGPGDHR
                     "
     gene            2495..2887
                     /gene="folB"
                     /locus_tag="VFE39_15860"
     CDS             2495..2887
                     /gene="folB"
                     /locus_tag="VFE39_15860"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HET6260112.1"
                     /translation="MTDRIELRGLKVRGNHGVFAHERRDGQDFVVDVTVWMDLAPAAA
                     SDDLTDTLDYGMLAQRVAAIVGGEPCDLIETVAGRVAENVMGDERVQAVEVVLHKPQA
                     PIPLEFADVSVVARRSRRSAPGARIIPA"
     gene            2884..3393
                     /gene="folK"
                     /locus_tag="VFE39_15865"
     CDS             2884..3393
                     /gene="folK"
                     /locus_tag="VFE39_15865"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET6260113.1"
                     /translation="MTRAVLSLGSNLGDRLAHLRAAVSGFADVLIAASPVYETAPWGG
                     VEQDDFLNAVIVVDDPGTDAWGWLRRGQDLENASGRVREVRWGPRTLDVDVVTVDGPD
                     GAVRSSDPELLLPHPGTPERATVLRPWLDIDASAELPGLGPVAALLGALGPGAESGMR
                     RRDDLVLVP"
     gene            3390..3851
                     /locus_tag="VFE39_15870"
     CDS             3390..3851
                     /locus_tag="VFE39_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3180 domain-containing protein"
                     /protein_id="HET6260114.1"
                     /translation="MTLTRPRHLLAVALVTALVVNLAVRLTYNSLPPLPLPAGATFGV
                     LGLAEVVAGNLLRARIRRRPGTQPVRPLVAARAVLVAKASSLAGAVMAGVWGGLLAHV
                     LPRAADVAAAASDALAAGVGLACALVLVGGALWLEWCCRTPDDESDEKLGE"
     gene            3893..>4618
                     /locus_tag="VFE39_15875"
     CDS             3893..>4618
                     /locus_tag="VFE39_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET6260115.1"
                     /translation="MMAQATMGVPLPTQKRQRRGVLAPVLGLVGLAICGLVVIGLVGS
                     TVGLGGVLVGALCALLPVGPVVATFLWIDRWEPEPPRLLLVAFLWGACFAALSALLVN
                     SSAVLVADQVLGQGSGDLVGTVAVAPVVEEAVKGAFLVGLLVFRRREFDGIVDGIVYA
                     GLVGGGFAFTENILYIGRAFADDAMVGQGSGVLAVLLLRGVLSPFAHPLFTVMTGIAA
                     GIASRSRSIPVRVVTVLIGYLLAV"
BASE COUNT          608 a         1609 c         1720 g          681 t
ORIGIN      
        1 cgaggagctc gtcttccacg agcccaccac cggcgcctcc tccgacatcg accaggctac
       61 gaagatcgcc aaggcgatgg tcaccgagta cggcatgagc gcgcggctcg gtgcggtgcg
      121 gtacggccgc gaccagggcg atccgttcct cggtcgctcg atgggcaacc aggccgacta
      181 ctcgctcgaa gtggcccacg agatcgacga ggaggtgcgc gcgctcatcg aggccgcgca
      241 cacggagtcg tgggagatcc tcaacaccta ccgggacgtc ctggacgacc tggtgttcga
      301 gctcctggag aaggagacgc tcacccgcag ggacctcgag cgaatcttcg accgggtgga
      361 gaagcgcccg cggatcaccg ccttcaatga cttcggcggg cgtaccccgt cggacaagcc
      421 tccgatccag acccctgccg agcgcgccag ggagcgcggc gagccgtggc cgccgcacac
      481 ggagccggca cgtgaccctg ctccagtcgg gtcctatccg ggcgggggtt acgcggtgcc
      541 cgcacccggc ccgcccaacg gtggcccggt gcccaccccg ggtgcgcccg gttaccccgg
      601 cggacccggc caaccgccgt tcccgacggc cgcccccggc gggtacgggc cgtccgcccc
      661 gcagcagacc tggggttccc cacccaatcc tgggcagggg cagcagccca acccgggcca
      721 accgaactcc gtgcagccca actacggggc gccggccgac tggcgccccg ccaccacccc
      781 ccacggccag tcctggccgc cggcggggca gtggcagcag cagccgcagc agccgcagca
      841 gtggccgccg cagcagtggc cgccggtggc cggtaacggc cacggcggtg atgggtctgc
      901 tgcagctccg tcccccgagc cgccgcacga gaacggcaag gccccgaacg aggacgggtc
      961 gcgctgagtc cgggagcgtt gggcgacacc ggggcctccg tggacggggc gttcgcaaga
     1021 gggccggcga acggcagacc ggcgatggac catgctcgcg ccgaggcggc ggtgcgcgag
     1081 ttgctgatcg cgatcggcga ggatcccgag cgcgacgggc tgcgtgacac acccgcgcgc
     1141 gtggcgaggg cctacgagga aatgttcgcc gggctctata ccgagccgga cacggtgctg
     1201 gagcggacgt tcgacgagca ccaccaggag ctcatcctcg tcaaggacat cccgatcttc
     1261 agcacgtgcg agcatcatct ggttcccttt cacggtatgg cgcacgtcgg ctacatcccg
     1321 aacaaggacg ggcgggtcac cggactgtcg aagattgcgc gtcttgtcga cctctacgcc
     1381 cggcgccccc aggtccagga gcggctcacc gggcaggtcg ccgatgcact ggtgcgcaag
     1441 ctggcccccc gcggtgtgat cgtcgtgatc gaggccgaac acctctgcat gggcatgcga
     1501 ggcatccgta agcccgggtc ccgcacggtg acgtcggcag tacggggcat gctccagtcc
     1561 tcgccgacgt cacgtgccga ggcgctcgcc ctcatccgga gcgggtgaga cacgtgggtc
     1621 cccgcacgga cccgccaggc ctgccccagc cggggcggtg cgtggtgatg ggcgtcctga
     1681 acgtcacgcc tgactcgttc tcggacgggg ggcgctacct cgaccgcgag cacgccgtgg
     1741 cgcacggcgt cgcgatgcac gaggcgggcg ccgatctcgt cgacgtcggt ggggagtcga
     1801 cgcgtccggg agccggtcgc gtcgaggccg gtgtggagaa ggcccgcgtc ctgccggtga
     1861 ttcgggagct ggtggcggag ggcgtgcagg tcagcatcga cacgaccagg gccgcggtcg
     1921 cggaggctgc cctcgaggcc ggggcgagca ttgtcaacga cgtctccggc gggctggccg
     1981 accgggcgat gccagccact gtggctcagg cgcgcgttcc gtggatcctg atgcactggc
     2041 gggggcacag cgaccggatg aacgcccttg ccacctacga ggacgtgctc ggcgaggtac
     2101 gcgacgagct ggtgtcgagg gtggacgccg cggtgcttgc cggcgtcgag ccctcgcggc
     2161 tggtgctcga tcccggcctg ggtttcgcga agaccgcggc gcacaactgg acgctcctgc
     2221 gcagactcga cgtgctgacc tcgctcggct tcccggtgct cgtgggcgcc tcgcgcaagc
     2281 gcttcctcgg ccagctgctc gccggtgccg acggctccct gcggccgttc gactaccggg
     2341 acggggccac ggcagcgatc agtgccctgg cggcctacaa cggcgcatgg ggggtgcggg
     2401 tgcacgacgt gcatggcaca ttggacgcgg tcgcggtggc caccgcgtgg cagctgggcg
     2461 cgtcgaaggc gcggaccggt ccgggggacc accgatgacc gatcgcatcg agctgcgtgg
     2521 gctgaaagtc cgcgggaacc atggggtgtt cgcgcacgag cgccgcgacg ggcaggactt
     2581 cgtcgtggac gtgaccgtgt ggatggacct ggcgcccgcg gcggcgtccg acgatctcac
     2641 cgacacgctc gactacggga tgctcgccca gcgggtggcc gcgatcgtcg ggggagagcc
     2701 gtgcgacctg atcgagacgg tggcgggacg cgttgccgag aacgtgatgg gcgacgaacg
     2761 tgtgcaggcg gtcgaggtcg tgctgcacaa gccgcaggcc ccgatcccgc tggagttcgc
     2821 cgacgtctcg gtcgtcgccc gccgctcccg ccgcagtgcg ccgggggcac ggatcattcc
     2881 ggcgtgaccc gcgccgtgct gtcgctcggg tccaacctcg gcgatcggct cgcgcacctg
     2941 cgggcggccg tcagtgggtt cgccgacgtg ttgatcgcgg cttcgcccgt gtacgagacg
     3001 gcgccgtggg gcggtgtcga gcaggacgac ttcctcaacg ccgtgatcgt cgtcgacgac
     3061 cccggcaccg acgcgtgggg ctggttgcgc cggggacagg acctggagaa cgcgtccggc
     3121 cgggtccgtg aggtgcggtg gggaccgcgg acgctcgacg tcgacgtcgt cacagtcgat
     3181 gggccagacg gtgcggtccg cagcagcgac cccgaactgc tgctcccgca tcccggcacg
     3241 ccggagcggg ccaccgtgct ccgcccctgg ctcgacatcg atgcgagcgc tgaactgccc
     3301 ggacttggtc cggtcgcggc gctgctcgga gccctcggac ccggcgccga gagcgggatg
     3361 cggcggcgcg acgacctggt cctcgtgcca tgaccctcac ccgcccgcgt cacctgctcg
     3421 ccgtcgcgct ggtgacggcg ttggtcgtga acctggcggt gcggctcacc tacaactcgc
     3481 ttccgccgtt gccgctcccg gcgggcgcga cgttcggcgt cctcgggctg gccgaggtgg
     3541 tggccggcaa cctcctgcgc gcacgtatcc ggcgccgtcc cggcacgcag ccggtgcgtc
     3601 cgctggtggc ggccagggcg gtgctcgtcg ccaaggcgtc gtcgctcgcg ggtgccgtca
     3661 tggccggcgt gtggggcggg ctgctcgccc acgtgctgcc ccgtgctgcg gacgtcgcgg
     3721 cggccgcgag cgacgcactg gccgctgggg tcgggctggc ctgcgcactc gtactcgtcg
     3781 gcggcgcgct gtggctcgag tggtgctgcc gcactccgga cgacgaatcc gacgagaagc
     3841 tcggcgagtg atgcgtgcag ggcaccacag cggccgcccg taggctcggc gcatgatggc
     3901 ccaggcgacg atgggcgttc cgctgcccac tcagaagagg cagcgccgcg gcgtgctcgc
     3961 cccggtgctg gggctggtcg ggctggccat ctgcgggctc gtggtaatcg gcctggtggg
     4021 cagcactgtc ggtctgggcg gagtcctcgt cggtgcgctc tgcgcgctgc tgccggtcgg
     4081 ccctgtcgtc gcgacgttcc tgtggatcga ccgctgggag cccgagccgc cacggctgct
     4141 gctggtggcg ttcctgtggg gagcatgctt cgctgctctc tcggcgctgc tggtcaactc
     4201 gagcgcggtg ctggtggccg accaggtgct cggccagggc agcggcgacc tcgtcgggac
     4261 cgtcgcggtc gcgccggtgg tggaggaggc ggtcaagggc gccttcctcg tcggcctgct
     4321 cgtcttccgc aggcgcgagt tcgacggaat cgtcgacggg atcgtctacg ccgggctggt
     4381 gggcgggggt ttcgccttca ccgagaacat cctctacatc ggccgggcgt tcgcagacga
     4441 cgcgatggtc gggcagggca gcggcgtgct cgccgtcctc ctgctgcggg gcgtgctctc
     4501 accgttcgcc catccgctgt tcaccgtgat gaccggtatc gccgccggca tcgcttcgcg
     4561 gagccgcagc atccccgtcc gcgtggtcac ggtgctcatc ggctatctcc tggcggtg
//
LOCUS       DASRSC010000324         9005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_789750,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000324 DASRSC010000000
VERSION     DASRSC010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9005
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789750"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="VFE39_15880"
     CDS             <1..1291
                     /locus_tag="VFE39_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HET6260116.1"
                     /translation="EVKATNGDNHLGGDDWDERIISWLVDRFKNGQGIDLTKDKMAMQ
                     RLREAAEKAKIELSSQSTATINLPYITVDADKNPLFLDETLSRAEFQRITSDLLDRTR
                     APFNQVIKDAGIAVGQIDHVVLVGGSTRMPAVTDLVKELTGGKEPNKGVNPDEVVAVG
                     AALQAGVLRGEVKDVLLLDVTPLSLGIETKGGVMTKLIERNTTIPTKRSEIFTTADDN
                     QPSVQIQVFQGEREIAAYNKKLGMFELTGLPPAPRGVPQIEVSFDIDANGIVNVSAKD
                     LGTGKSQAMTITGGSALGKDEIDRMMREAEQHAEEDKKRREEAEVRNQAESLVYQTEK
                     FVKENDEKLPSDVKDGVNAALGEAQEALKGTDTDAIKSSMEKLATESQKMGTALYQQP
                     GADGAAPGADAGQAQGNNAGDDDVVDAEIVDEEKDKK"
     gene            1288..1962
                     /gene="grpE"
                     /locus_tag="VFE39_15885"
     CDS             1288..1962
                     /gene="grpE"
                     /locus_tag="VFE39_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET6260117.1"
                     /translation="MNDHDRNERGENGADGERVVVRDRRRIDPVTGEVRVPVGEPAGT
                     APTEAPEADTGLDAVAAAELAAQVAERTSDLQRVSAEYANYRRRVDRDRESVLVGARV
                     QFVSELLTVLDDLDRAEAHGDLTGPFKAVADKLVAVVQKLGLEPFGLEGEPFDPSVHE
                     AVQHEDSGVDGPSITVVSTVLRRGYRIADRVLRPAMVMVADRADDGTGTEPAPTTTEP
                     DPAEGP"
     gene            1982..3190
                     /gene="dnaJ"
                     /locus_tag="VFE39_15890"
     CDS             1982..3190
                     /gene="dnaJ"
                     /locus_tag="VFE39_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET6260118.1"
                     /translation="MTQRDWIEKDFYRELGVASGASADDIKKAYRKLARELHPDANPG
                     DANAEARFKSVSEAYGVLSDEKKRREYDETRALFAGGGGFGGGGGFPGGFGSGASGQG
                     FDLNDLFGRAGASGAGGSAGAGGLGDILGGIFGNRAGTGPTTTGARAQRGADVESELR
                     IDFVDAVRGVEVQLRLSSPGRCERCGGTGSRPGSTPRQCPTCGGVGLVSRSQGAFAFS
                     EPCRDCRGTGRIIDDPCPECGGDGVSTRTRLLTVRVPAGVADGQQVRLKGQGEPGRGG
                     APAGDLYVKVHVNSHRLFGRSERNPDDLTLTVPVTYPELVLGSTLTVPTLGDPVSVRI
                     PAGTASGRTFRVRGRGVQRKGGKTGDLLVTVEVAVPSSLDDAATESLQAYAEATKSFD
                     PRADLLGGRR"
     gene            3187..3624
                     /locus_tag="VFE39_15895"
     CDS             3187..3624
                     /locus_tag="VFE39_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6260119.1"
                     /translation="MTAGGPALPPGANPDSPVFVISVAAELAGLHAQTLRSYDRLGLV
                     SPGRSPGGGRRYSVRDIALLREVQRLSQEEGVNLAGIKRIIELEELVDELRARLDDMT
                     RELLAAHSAAEQAAASVHASYRRDLVPVDRRQALVVWRPKPRA"
     gene            complement(3869..5041)
                     /locus_tag="VFE39_15900"
     CDS             complement(3869..5041)
                     /locus_tag="VFE39_15900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HET6260120.1"
                     /translation="MTAEQLLTADLPNVGESRSEQSGMVEIIRSSFAMVEPRAHELGR
                     HFYATLFRIAPETRDLFPVNMEVQRSRLLRALVHVVQMVDQPDDLVPFLEQLGRDHRK
                     FGVLAQHYDAVGAALLGTIALHAGAAWTPEVQKAWTDAYGIVAGAMITAAQAERGPAS
                     WLGRVLEHTRVGWDLAMITVQASEPIPYRAGQYLSVETPQRPRLWRYLSPANAPRADG
                     VLEFHVRAVDGGWVSRAIVAHSRVGDTWRIGPPMGRMHVEPGSGRDLLMVTGGTGMAP
                     IKALLEDLAARPETPHTQVFVGGRTWDDLYDFPSLRRLSYSNTWLDVIPVVEKDEGGS
                     GAERGTLADVVTRYGAWADHDVLVCGSPAMIRSTVSRMLVAGTPLDRIRYDPFTMD"
     gene            5451..8081
                     /gene="clpB"
                     /locus_tag="VFE39_15905"
     CDS             5451..8081
                     /gene="clpB"
                     /locus_tag="VFE39_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HET6260121.1"
                     /translation="MDSFNPTTKAQQAISAAVQAAALAGNPDVGAPHLLGALLAQGDG
                     IAVPLLTAVGADVAAVRADLAQLVNRLPSAAGATVSAPQLSRDAIAAINAAQQLATEM
                     GDEYVSTEHLLVGLASAPGRVGQSQVADLLRRHGATPEALREAFAKVRGSSRVTSPDP
                     EGTYQALEKYGVDLTERARKGELDPVIGRDTEIRRVVQVLSRRTKNNPVLIGEPGVGK
                     TAIVEGLAQRIVAGDVPESLRGKRVVALDLGSMVAGAKYRGEFEERLKAVLKEITDSA
                     GQVITFIDELHTIVGAGATGEGAMDAGNMIKPMLARGELRMVGATTLDEYRQRIEKDP
                     ALERRFQQVFVGEPSVEDTVGILRGLKERYEVHHGVRITDAALVAAAALSDRYITARF
                     LPDKAIDLVDEAASRLRMEIDSRPVEIDTVERAVRRLEIEEMALEKESDAASVERLAA
                     LRAELAEQRERLSALTARWHNERDAIESVRELKEQLEALRGESERAERDGELGRAAEL
                     RYGKIPTLEKQLAEATAVTAPDADVMLKEEVGPDDVADVVSAWTGIPAGRMLEGETAK
                     LLRMEDELAKRVVGQSEAVRAVSDAVRRARSGIADENRPTGSFLFLGPTGVGKTELAK
                     ALAEFLFDDERAMVRIDMSEYSEKHSVARLVGAPPGYVGYDQGGQLTEAVRRRPYTVV
                     LFDEVEKAHPDVFDTLLQVLDDGRLTDGQGRTVDFRNTILVLTSNLGSQAIADPSLDD
                     RGRRDAVMSNVQRHFKPEFLNRLDDVVVFHALSTEELTHIVDIQLDVLRRRLAKRRLT
                     LEVSDAGREWLAMNGFDPVYGARPLRRLVQSAIGDQLAKELLSGDVREGDTVRVDLDP
                     TADGGMGGLIVGRGLPVTMS"
     gene            8212..8871
                     /locus_tag="VFE39_15910"
     CDS             8212..8871
                     /locus_tag="VFE39_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260122.1"
                     /translation="MGLSSTVWFVIAVIALLLGAALLFADRRGGSGGAKDRKRWAAAH
                     GWEYLDTDPVLPSRWRYGTIHQGGPGVARNLMSGDVPTSYGSRHAYVFDHEQAGRLNS
                     VLAAVQVQDQLPAAVELRLPSAPLPDDAGLDLLEPVGERYAFVSDAEAVRPLLTPRLA
                     EASDRVGDDVELLWAEESWVLATAPLGSSAERVQALLADLADVATVLEQSLGVGPGVD
                     R"
BASE COUNT         1374 a         3092 c         3290 g         1249 t
ORIGIN      
        1 cgaggtgaag gccaccaacg gtgacaacca cctcggtggc gacgactggg acgagcggat
       61 catcagctgg ctcgtcgacc ggttcaagaa cggccagggc atcgacctga ccaaggacaa
      121 gatggcgatg cagcggctgc gtgaggccgc ggagaaggcg aagatcgagc tgtcgagcca
      181 gtcgaccgcc acgatcaacc ttccctacat cacggtcgac gcggacaaga acccgctgtt
      241 cctcgacgag acgctgtccc gggccgagtt ccagcggatc acctccgacc tgctcgaccg
      301 cacccgtgca ccgttcaacc aggtcatcaa ggacgccggt atcgccgtcg gccagatcga
      361 ccacgtggtg ctcgtcggtg gttccacccg catgcccgcc gtcaccgatc tcgtcaagga
      421 gctgaccggc ggcaaggagc ccaacaaggg cgtcaacccg gacgaggtcg tcgccgtcgg
      481 cgccgccctg caggccggtg tgctgcgcgg tgaggtcaag gacgtcctgc tgctcgacgt
      541 caccccgctg tccctcggca tcgagaccaa gggcggcgtg atgaccaagc tcatcgagcg
      601 caacaccacg atccccacca agcggtcgga gatcttcact acggccgacg acaaccagcc
      661 gtccgtgcag atccaggtgt tccagggcga gcgtgagatc gcggcctaca acaagaagct
      721 cggcatgttc gagctgacgg gtctgccgcc cgctccccgc ggcgtgccgc agatcgaggt
      781 ctccttcgac atcgacgcca acggcatcgt caacgtgtcc gccaaggatc tgggcaccgg
      841 caagagccag gccatgacga tcaccggcgg atccgcactg ggcaaggacg agatcgaccg
      901 gatgatgcgc gaggccgagc agcacgccga ggaggacaag aagcgtcgcg aggaggccga
      961 ggttcgcaac caggccgagt cgctcgtcta ccagacggag aagttcgtca aggagaacga
     1021 cgagaagctc ccgtccgacg tcaaggacgg cgtcaacgcc gctctcggcg aggcgcagga
     1081 ggcgctgaag ggcaccgaca ccgacgcgat caagtcgtcg atggagaagc tcgccaccga
     1141 gtcccagaag atgggcaccg cgctgtacca gcagccgggt gccgacggtg ctgctccggg
     1201 cgccgatgcc gggcaggcgc agggcaacaa cgccggcgac gacgacgtcg tggacgccga
     1261 gatcgtcgac gaggagaagg acaagaagtg aacgaccacg accgcaacga gcgcggcgag
     1321 aacggcgccg acggcgagcg ggtcgtggtc cgggaccggc gccggatcga cccggtgacc
     1381 ggggaggtgc gggtcccggt cggcgagccg gccgggaccg cccccaccga ggcaccggag
     1441 gccgataccg gcctggatgc cgtcgccgct gccgagcttg ccgcccaggt ggccgagcgc
     1501 acgtctgacc tgcagcgggt cagcgcggag tacgccaact atcggcgccg cgtcgaccgt
     1561 gaccgcgaga gcgtgctggt cggggctcgg gtgcagttcg tctccgagct gctcaccgtg
     1621 ctcgacgacc tggatcgggc cgaggcgcac ggtgacctca ccggcccgtt caaggccgtc
     1681 gccgacaagc tggtggccgt cgtacagaag ctcggcctgg agccgttcgg gctggagggt
     1741 gagcccttcg acccgtccgt ccacgaggcg gtgcagcacg aggacagcgg ggtcgacggg
     1801 ccgagcatca cggtggtctc gacggtgctc cgtcgcggct atcggatcgc cgaccgcgtc
     1861 ctgcggcccg ccatggtgat ggtcgccgac cgcgccgacg acggcaccgg taccgagcct
     1921 gctccgacca ccaccgaacc cgacccggcc gaggggccct gagcagacgc gaggaggcgg
     1981 agtgacccag cgcgactgga tcgagaagga cttctaccgc gagctgggcg tcgcctccgg
     2041 cgcgtccgcg gacgacatca agaaggcgta ccggaagctc gccagagagc tgcacccgga
     2101 cgccaacccc ggcgacgcca acgccgaggc gcggttcaag tccgtgtccg aggcgtacgg
     2161 ggtgctgtcc gacgagaaga agcgccgtga gtacgacgag acacgcgcgc tgttcgcggg
     2221 tgggggtggt ttcgggggcg gcggtgggtt ccccggcggt ttcggcagcg gcgcatccgg
     2281 ccagggcttc gacctgaacg acctgttcgg tcgcgccggt gcttccggtg ccggcgggtc
     2341 ggccggcgcc ggaggtctgg gcgacatcct cggcggcatc ttcggcaacc gtgccgggac
     2401 gggccccacc acaacgggcg cccgggccca gcgcggtgcc gacgtcgaga gcgagctccg
     2461 aatcgacttc gtcgacgccg tgcgtggtgt cgaggtgcag ctgcggctgt cgtcgccggg
     2521 gcgctgcgag cggtgcggtg gtaccgggtc ccgccccggc agcacgccgc ggcagtgccc
     2581 gacgtgtggc ggcgtcggtc tggtcagccg cagccagggc gcgttcgcgt tctccgagcc
     2641 gtgccgcgac tgccgcggca ccggccggat catcgacgac ccgtgccccg agtgcggcgg
     2701 cgacggcgtc agcacccgca ctcgtctgct caccgtccgc gtcccggccg gggtggcgga
     2761 cgggcagcag gtgcggctca aggggcaggg cgagcccggc cggggcggtg ctccggccgg
     2821 tgacctgtac gtgaaggtgc acgtcaactc gcaccggctg ttcgggcggt cggagcgcaa
     2881 tccggacgac ctgaccctca cggttccggt cacctacccg gagctggtgc tcggcagcac
     2941 gctgaccgtg cccacgctcg gggaccccgt gtcggtgcgg atccccgccg gtacggcgag
     3001 cgggcgcacg ttccgcgtgc gtgggcgcgg cgtgcagcgc aagggcggaa agacgggcga
     3061 cctgctggtc accgtcgagg tggcggtgcc gagctcgctg gacgacgcgg ccaccgagtc
     3121 gctgcaggcc tacgccgagg ccaccaaatc gttcgacccg cgcgccgacc tgctcggagg
     3181 ccggcgatga ctgcgggcgg tccggcgctg cccccgggtg ccaacccgga cagcccggtg
     3241 ttcgtgatct cggtggcggc ggagctggcc gggctgcacg cccagacgct gcgcagctac
     3301 gaccggctgg ggctcgtgag ccccggccgg tcgccgggcg gcggtcggcg ctactcggtt
     3361 cgcgacatcg ccctgctccg cgaggtgcag cgactctccc aggaggaggg cgtgaacctc
     3421 gcgggcatca agcggatcat cgagctggag gagctcgtcg acgagctccg ggcccgtctc
     3481 gacgacatga cccgggagct gctcgccgcg cacagcgcgg ccgagcaggc ggcagcgagc
     3541 gtccatgcgt cctaccgtcg ggatctcgtg ccggtggatc gccggcaggc cttggtggtg
     3601 tggcggccga agccgcgggc gtgacgtccc gtctcagccc gtcggctgga cgaccatccg
     3661 cccggtggtc gtcccgccgg tgggagtact caggtgcggg atcacaagat cctggacgtc
     3721 ggcgtacggc cacccgccgt cgagagcaca actgtggtcc ggccaccgtg agtctgaggt
     3781 tctcgggcag tatcaggtcc agctggccgc acgatgtgcg ccagcatgtc gggcaggacg
     3841 gccgcccgac tctctgaccc agctctcatt aatccatcgt gaacgggtcg taccggatgc
     3901 ggtcgagggg cgtgcccgcc acgagcatcc gggacacggt cgaccggatc atggccggtg
     3961 agccgcagac cagcacgtcg tggtcggccc acgccccgta ccgggtgacg acgtccgcca
     4021 gggtgccgcg ctcggccccg gagcccccct cgtccttctc cacgacgggg atgacgtcca
     4081 gccaggtgtt gctgtaggac agcctgcgca ggctggggaa gtcgtacagg tcgtcccagg
     4141 tgcgcccgcc gacgaacacc tgcgtgtggg gtgtctccgg ccgcgccgcc aggtcctcga
     4201 gcagcgcctt gatcggcgcc atgccggtgc cgccggtgac catcaggagg tcccgccccg
     4261 atccgggctc cacgtgcatg cggcccatcg gcggaccgat ccgccaggtg tcgccgaccc
     4321 gcgagtgggc gacgatggcg cggctcaccc agccgccgtc gaccgcgcgg acgtggaact
     4381 ccagcacccc gtcggcgcgg ggcgcgttgg ccggggagag gtagcgccac aggcgcggcc
     4441 gttgtggtgt ctcgacgctc aggtactggc ccgcccggta cggaatcggc tcgctggcct
     4501 gcacggtgat catcgcgagg tcccagccga cccgcgtgtg ctccagtacg cgcccgagcc
     4561 aggatgccgg gccgcgctcg gcctgtgcag cggtgatcat ggcaccggcc acgattccgt
     4621 acgcgtcggt ccaggctttc tgcacctccg gcgtccaggc ggcgcccgcg tgcagggcga
     4681 tcgtgccgag cagcgccgcg ccgacggcgt cgtagtgctg ggcgagcacc ccgaacttgc
     4741 ggtggtcgcg gccgagctgc tcgaggaagg gcacgaggtc gtcgggctgg tcgaccatct
     4801 ggacgacgtg cacgagcgcc ctgagcaggc ggctgcgctg cacctccatg ttgacgggga
     4861 acaggtcccg cgtctccggg gcgatccgga acagcgtggc gtagaagtgc cgtccgagct
     4921 cgtgggcgcg tggctcgacc atcgcgaagc tggagcggat gatctcgacc atcccggact
     4981 gctcgctgcg ggactcaccg acgttgggga ggtcggcggt gagcaactgt tcagcggtca
     5041 tgaaaggggc cggtacttcc tgctcggtgt cgggggaagg gacgtgggtc ccgggtgagg
     5101 cagcctaacg cccgagagac ggtctggtga ccctcgttgc ccaccggtaa cgcggttcac
     5161 ggggtacagc cgtccgtcca cccgctcgtc ccggccccgg cgaccgttcg gcgcacggcg
     5221 attcgggcgc ccgtcgacga gcacaacggc cacgcaaggt ccccggaaag cggtgacttc
     5281 atcgccctca cgggccttcc gggcaccccg caccctgagc aacgatgcgg gcgggtctca
     5341 gccgtcgacg gcgggagagc tgagctgagc ggaacacgct caactttcct gacgttgtga
     5401 cgatcgaagg gcacgatggt gtccaggcga caatgcagga ggaacagggg atggactcct
     5461 tcaaccccac caccaaggcg cagcaggcca tctcggccgc ggtgcaggcc gctgcgctcg
     5521 ccggcaaccc cgatgtcggc gccccgcact tgttgggggc cctgctcgcg cagggcgacg
     5581 ggatcgccgt gccgctgctc acggcggtcg gcgcggacgt ggcggccgtg cgcgccgatc
     5641 tcgcgcagct ggtcaaccgg ctgccctccg cggccggcgc caccgtgagc gcaccgcagc
     5701 tgtcccggga cgcgattgcc gcgatcaacg cggcccagca gctcgccacc gagatgggtg
     5761 acgagtacgt ctccaccgag catctgctcg tcgggctcgc gtcggcgccc ggtcgagtgg
     5821 ggcagagcca ggtcgccgac ctgctgcgcc gccatggcgc cacgcccgag gcactgcgcg
     5881 aggcgttcgc gaaggttcgc ggctcgtccc gggtcaccag ccctgatccg gagggcacct
     5941 accaggcact ggagaagtac ggcgtcgacc tcaccgagcg ggcccgcaag ggcgagctcg
     6001 accccgtgat cggtcgcgac accgagatcc gtcgcgtcgt gcaggtgctg tcgcggcgca
     6061 cgaagaacaa cccggtgctg atcggcgagc cgggcgtcgg caagacggcg atcgtcgagg
     6121 ggctggccca gcggatcgtg gcgggcgacg tcccggagtc gctgcgcggc aagcgcgtcg
     6181 tggcactcga cctcgggtcg atggtcgccg gcgcgaagta ccgcggtgag ttcgaggagc
     6241 ggctgaaggc cgtcctcaag gagatcaccg actccgcggg ccaggtcatc acgttcatcg
     6301 acgagctgca cacgatcgtc ggggcgggtg ccaccggcga gggcgcgatg gacgccggaa
     6361 acatgatcaa gccgatgctc gcccgcggtg agctgcggat ggtcggagcc accacgctcg
     6421 acgagtaccg ccagcgcatc gagaaggatc cggcgctgga gcgccggttc cagcaggtgt
     6481 tcgtggggga gccgtcggtg gaggacacgg tcggcatcct ccgtgggctc aaggagcgct
     6541 acgaggtgca ccacggcgtg cggatcaccg acgctgcgtt ggttgcagct gccgcgttgt
     6601 ccgaccggta catcaccgcc cgtttcctcc cggacaaggc gatcgacctg gtcgacgagg
     6661 ccgcctcgcg gctccggatg gagatcgact cgcggcccgt cgagatcgac accgtcgagc
     6721 gcgctgtgcg ccgactggag atcgaggaga tggcgctgga gaaggagtcc gacgcagcgt
     6781 cggtcgagcg gctcgcggcg ctgcgggccg agctggccga gcagcgcgag cggctctccg
     6841 ccctcaccgc ccgttggcac aacgagcgcg acgccatcga gtcggtacgc gagctcaagg
     6901 agcagctgga ggcgctgcgt ggggagtcgg agcgtgccga gcgcgacggc gagctcggcc
     6961 gggccgccga attgcgctac ggaaagattc ccacgctgga gaagcagctg gccgaggcca
     7021 cggccgtcac cgctccggac gccgatgtga tgttgaagga ggaggtcggc ccggacgacg
     7081 tggccgacgt cgtctccgca tggaccggca tcccggcagg ccgcatgctc gagggcgaga
     7141 cggccaagct gctgcgcatg gaggacgagc tcgcgaagcg cgtcgtcggc cagtccgagg
     7201 ccgtacgcgc cgtgtccgac gccgtgcgca gggccaggtc gggcatcgcc gacgagaacc
     7261 ggcccaccgg atcgttcctg ttcctcggtc ccaccggtgt gggcaagacc gagctggcca
     7321 aggctctcgc ggagttcctg ttcgacgacg agcgggcgat ggtccggatc gacatgagcg
     7381 agtactcgga gaagcacagc gtcgcccggc tggtcggtgc accacccggc tacgtcggtt
     7441 acgaccaggg tgggcagctc accgaggccg ttcggcgccg cccgtacacc gtggtgctgt
     7501 tcgacgaggt cgagaaggcg caccccgacg tcttcgacac gctgttgcag gtgctcgacg
     7561 acggcaggct gaccgacggc cagggccgca cggtcgactt ccgcaacacg atcctggtgc
     7621 tcacctccaa cctcggttcg caggcgatcg ccgacccgtc gctcgacgac cgcggcaggc
     7681 gcgacgccgt gatgtcgaac gttcagcggc acttcaagcc ggagttcctc aaccggctcg
     7741 acgacgtggt cgtcttccac gcactgtcca cggaggaact cacgcacatc gtcgacatcc
     7801 agctcgacgt gctgcgccgg cgcctggcga agcgcaggct cacgctggag gtctccgacg
     7861 ccggacgcga gtggctggcc atgaacggct tcgaccccgt ctacggggca cgtccgctgc
     7921 gcaggctcgt gcagtccgcc atcggcgatc agcttgcgaa ggagttgctg tccggcgacg
     7981 tccgcgaggg cgacacggtg cgcgtcgacc tcgaccccac cgcggacggc ggcatgggcg
     8041 ggctgatcgt gggccgcggt ctgccggtca cgatgtcctg agccctgcca tcggtacggc
     8101 ccggcgcgaa ccggcgtcct ggagcgccgg ctcgcgccga ttcacccaca ggggtcagcg
     8161 ctggttcgtg gcatctgcgc acttccggcg ccatcggtct aggctccgcc cgtgggcctc
     8221 tcgtcgacgg tgtggttcgt catcgccgtg atcgcgctgc tcctcggcgc cgcgctgctg
     8281 tttgccgacc gccgtggcgg gtcgggtggc gccaaggatc ggaagcgctg ggccgccgcg
     8341 cacggctggg agtacctgga cactgatccg gtgcttccga gccggtggcg ctacggcacg
     8401 atccaccagg gcgggccagg tgtggcgcgc aatctgatga gtggcgacgt gcccacgtcc
     8461 tacgggtcgc ggcacgccta cgtgttcgac cacgagcagg ccggcaggct caactccgtg
     8521 ctggcggcgg tgcaggttca ggaccagttg cccgccgcgg tcgagctgcg gctgccgtcg
     8581 gccccgctgc ccgacgacgc cggtctcgac ctgctggagc cggtcggcga acggtacgcg
     8641 ttcgtgtccg acgcggaggc cgtgcgaccc ctgctcacgc cacgcctggc ggaggcgagc
     8701 gaccgggtcg gggacgacgt ggagctgctg tgggcggagg agtcctgggt gcttgcgacg
     8761 gcgccgctcg gctccagcgc ggagcgggta caggcgctgc tggccgacct cgcggacgtg
     8821 gccaccgtgc tcgagcagtc gctcggtgtc gggccgggcg tggaccgctg accttctcgg
     8881 acgcgcccat ccggccgcga ggtggcgcgc gggtggtccg tcactaggct cgcgccgtgc
     8941 ccaccgcgct gatcacagga gccaccgcag gcatcggggc cgcgttcgcg tcccgactcg
     9001 cacgc
//
LOCUS       DASRSC010000325        10881 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5542911,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000325 DASRSC010000000
VERSION     DASRSC010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10881
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5542911"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..126
                     /locus_tag="VFE39_15915"
     CDS             1..126
                     /locus_tag="VFE39_15915"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6260123.1"
                     /translation="MGLGATRVSHEPELPWVVLADPEGNEFCVLPPRSDGSDQAE"
     gene            complement(114..578)
                     /locus_tag="VFE39_15920"
     CDS             complement(114..578)
                     /locus_tag="VFE39_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HET6260124.1"
                     /translation="MSDPVPTLYDWCGGSAALHRLTEVFYDRVLADPVLEPVFRHMGA
                     DHRDHVARWLGEVFGGPTDYTHQLGGYPAMLQHHLGLGITEEQRARWAALIATSADEA
                     GLPDDPEFRSAFVAYVEWGTRIARANSVPGATPPEHAPTPRWGWGEAPPYSA"
     gene            complement(604..1344)
                     /locus_tag="VFE39_15925"
     CDS             complement(604..1344)
                     /locus_tag="VFE39_15925"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HET6260125.1"
                     /translation="MDAPSLDLAADLVGGWLQLPGSASAELMGSVGFDFVCVDQQHGL
                     IGDDALLPMAQALAATGTPVVVRVTGNEPSAIGRALDRGAAGVIVPLVDTAQDAARAV
                     AACHYPPRGGRSYGPTRRAWSPGGPDPQCIVMVETLAAVQALPDIVAVEGLDAVFVGP
                     SDLALSAGLPLGAQYDDPGFERLLHEIVDECRSRDLPVGIYSASPAHVHRFRELGFTF
                     FTLLSEAAILRAAALAHLTNARASGVTL"
     gene            1460..2419
                     /locus_tag="VFE39_15930"
     CDS             1460..2419
                     /locus_tag="VFE39_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HET6260126.1"
                     /translation="MSDIAPHGLHHVTAIASDPQRNVDFYTRVLGLRLVKQTVNFDAP
                     DTYHLYYGDTQGTPSTILTFFPWQGVPSGRQGSGLTTATAFSVPPESMGWWQRHLTGL
                     GLELDGPRTRDGEEVLTLRDPDGMVIDLVAAAGDARSGWDGVAGVPAEHAVRGLHAVT
                     MSERLPEATAELLSATLGMRLQGEDGDRTRFAMGAGGTGAVVDVAADSRPRGLQAGGT
                     VHHIAFRAPDQATQARWRQELVEAGLHVTEIIDRQYFTSIYFHEPGGVLLEIATDQPG
                     FTVDEPLLELGRSLKLPPWLEPSREQIAAALPPLRVDEPVESS"
     gene            2416..3057
                     /locus_tag="VFE39_15935"
     CDS             2416..3057
                     /locus_tag="VFE39_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6260127.1"
                     /translation="MREAATGPTLQRPHVWLPGTGGPPLLFLHGTGGDEHDMLPLRGH
                     LAPDAPVLSPRGTVLENGMPRFFRRLREGVFDEDDLRERTDELAEFLTAAGTEHGVAP
                     GSWVAVGFSNGANIASALLFRRPEALAGAVLLAAMVPFGDVPGLDVNLTGKRVAVVNG
                     RRDPMATAAQTRTLVAQLRDRGAAVVEFPHDGGHWIDVAVLPRIREFLAAGPA"
     gene            complement(3118..4452)
                     /locus_tag="VFE39_15940"
     CDS             complement(3118..4452)
                     /locus_tag="VFE39_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6260128.1"
                     /translation="MIRRSRRSTLAAAVVAAALVLSACGAGGRPDGGSGGEGGPATVG
                     ITATSVKIGAHFPLTGVAAPGYSEIPTGAKAYFDFVNAAGGVNGRKIDYVFRDDAYNP
                     TQTSQVVNELVLQDQVFALLSGLGTPTHSAVLDYLNSEGVPDLFVSSGSLLWDQPAKN
                     PQTFGWQPDYEIEGKIVGQYVAQNFPQAKVGLFLQDDDFGRDGELGARRYLGAQIVAA
                     ERYTPGNTDVAPQIAALQAAGADLVIGFNVPSYTALSQLQSLRLNYRPQWIYSNVGSD
                     PGLVGSLLARFSQGAVTDAGLLDGVITTEYLPGVDTPDNPWTQMFQRVWDEHGGDGEL
                     TNYRIYGMSQAYTFVQALQAAGPDPTRQGVVQAVERAGAQFQGPWVAPFRFSADRHAG
                     ISGMEVTRITGGKTESLTPVLVTDNGDAPITQSTSRPTTPPANGIPAVPAAG"
     gene            complement(4449..5495)
                     /locus_tag="VFE39_15945"
     CDS             complement(4449..5495)
                     /locus_tag="VFE39_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6260129.1"
                     /translation="MPRSTFGRHALITLAALVAVVLVLELTSPFRNSQFATMSYYAIA
                     AAGLTVLTGLNGQISLGHGALMAVGAYTTGLLLTGDGGLPLPVVMLVAAAVTALVGAV
                     VGAAAARLHGPYLAGATLALAVAVPGLAIYFDTALGGEQGLPVTSPHVPGWFADAVYF
                     LSANETPTAKYLAYVGWGTLLLVLLLLANLMAGRTGRVWRAVRDDEVAAELAGIRLGR
                     VRVLAFVVSAACAGLAGAVLGIVVRLAAPSGFTIVLSLGLLTAVVVGGLGSLVGALLG
                     SALLVFLPPAVTNIGIGAGLDGTQAAQLAPFVYGVVLVLVMLLAPSGAVGVMKKLWNR
                     SRRNRRGPSTRRTG"
     gene            complement(5606..6484)
                     /locus_tag="VFE39_15950"
     CDS             complement(5606..6484)
                     /locus_tag="VFE39_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005452321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET6260130.1"
                     /translation="MQQFLNVTLTGLGQGAVYAAFALALVLIWRSTRIVNFAQGAMAM
                     FTTYLALTVIEGGQSYWVGLAVALVTGLVLGAVVERLVIRPVEGGPELNAVIVTLGLF
                     VALQALAAILFGSTFRSFPAPFDLRGFAVGGINIAFTANSIYTIAAVVVVMLALVALF
                     RFTDLGLKMRAAAFSQEVSRLLGVPVGRMLTLGWALAAVVGSLSGLLIAGGSLVFPAY
                     MDAVIVYGFVAAVLGGLDSPGGAVVGGLTIGLLLSYVSGYLGSELVALAALAILVAVL
                     LVRPQGLFARTAARRV"
     gene            complement(6513..7289)
                     /locus_tag="VFE39_15955"
     CDS             complement(6513..7289)
                     /locus_tag="VFE39_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6260131.1"
                     /translation="MSEPASPSSAVRTGTLEVRGLTTAYGPVTALDAVDITAERGKVT
                     AVLGANGAGKSTLLRTISGLVRPDAGQVLLDGTDLTRAPAEAIARAGIAHVPEGRGVI
                     TELTVEENLRLGALLGASRRRAGSMPQSDRAGRSLAQVYEMFPPLAERRRRDAHVLSG
                     GERQMLVIGRALLSGPDVLLLDEPSLGLAPRIVAQIFGLLRDLVDSDGVTVLLVEQNA
                     RSALSIADVGIVLNLGRVVARDSAAALAADDGLRHAYLGF"
     gene            complement(7286..8029)
                     /locus_tag="VFE39_15960"
     CDS             complement(7286..8029)
                     /locus_tag="VFE39_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6260132.1"
                     /translation="MGVRFGGLVALDDVSLAVGAGQVHGVIGPNGAGKTTLFNVCCGF
                     VRPDTGTVLRRGKPVRDLRPHRLAGMGIARTLQGVGLFTGLTVAENVMVGADRHRRSG
                     FAATLFALARSDRDERALRERAMAALCELGADGYADRLPGSLPYPVQKRVALARALAA
                     EPDLLLLDEPASGLSNDEMTELGELIRTLTDRMSVVLVEHHMDLVMSVCDRITVLDFG
                     RVVAEGTPDEVRDDPAVIDAYLGEEATHS"
     gene            8297..9601
                     /locus_tag="VFE39_15965"
     CDS             8297..9601
                     /locus_tag="VFE39_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019815573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6260133.1"
                     /translation="MLPTGRSAPGTRINAGGDRVDDAGSAGAAPTYSASRAPARPLPD
                     ESSEALRVLGGVLLDEVDGLADRLTVMVLQREPVYAELDMVEELRGACRVNLERGVQV
                     LAEQVPGGVDPEDTSRQTGRRRARQGVPLEVVLRAYRLGGRVMWEALLGASRGRFSGR
                     YDDVLLDAASYVWRVNDSSSSALVEAYRLEELRLQSHDLSRRHAVLDGLIEGRGSDPA
                     FLRDAATVLGLPERGPVLCVVAPVEPSGEDPLRSPQEALSAHGLVSSWFLRPRDEVGV
                     VALAARPHEVALDALRPCAEGRVGVSPVVDGLGAIDAGYRMAHTAARTLSGPGLAVLD
                     ERLPEALLVDSPELVPRVVRTGLGSLLDLPVVDRDMLLETLQELLAHGGSPTYAAAAL
                     FCHRNTVIYRMRRIEAATGRSMGDPRDRLLLTLGVMAARMGA"
     gene            complement(9617..10792)
                     /locus_tag="VFE39_15970"
     CDS             complement(9617..10792)
                     /locus_tag="VFE39_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016333140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HET6260134.1"
                     /translation="MTIGTAERRARLGRRHLLAPATGAPGSVAGVVGVADALVALHAT
                     DPASVVLAVLARSGADVPTVERVLHDERALVRMLSMRRTVWVVALDVAPVVQAACTLD
                     VAAVQRRRLVEHIGRSGVTDEPAAWLADVEASTLKALLERGEATAVELVADEPRLRTR
                     LAVPPDDTAQNMTSRAMLLLSADGHIVRGRPLGGWTSTQYRWSPVSAWWPDGMPELEQ
                     GAARTELARRWLTVFGPAPVADLKWWTGWTMARTRAALAALGAVPVDLDGVPGVVLPH
                     DVEPVPDPGPWVALLPALDPTPMGWQDRAWFLGPHKAALFDRSGNIGPTVWCDGRIVG
                     GWAHRADGTVALRLLEDVGSEATAAVHAAAERLAARIGDVRVTPRFRTPLERELVAS"
BASE COUNT         1550 a         3864 c         4051 g         1416 t
ORIGIN      
        1 gtggggctgg gcgccacgcg cgtctcgcac gagcccgagc tcccgtgggt ggtgctcgcc
       61 gaccccgagg gcaacgagtt ctgcgtcctg cccccgcgct cggacggatc ggatcaggcg
      121 gagtagggag gggcctcgcc ccagccccac cgcggtgtgg gcgcgtgctc ggggggagtg
      181 gcgccgggca ccgagttggc gcgggcgatc cgggtgcccc actcgacgta ggcgacgaag
      241 gcggagcgga actcggggtc gtcgggcagg ccggcttcgt cggcgctcgt ggcgatgagt
      301 gccgcccagc gggcccgctg ctcctcggtg atcccgaggc cgagatggtg ctgcagcatc
      361 gccgggtagc caccgagctg gtgcgtgtag tccgtggggc cgccgaacac ctcgccgagc
      421 cagcgggcga cgtggtcgcg gtggtcggca cccatgtgtc ggaacacggg ctcgagcacg
      481 gggtcggcga ggacccggtc gtagaacacc tcggtcaacc gatgcagggc agcggaaccg
      541 ccgcaccagt cgtagagcgt ggggacgggg tcggacacgg cagcgctccc atcgattacc
      601 gaattacagc gtaacaccgc tggcgcgagc gttggtcagg tgtgccaggg ccgccgcccg
      661 gagaatcgcc gcctcggaca ggagtgtgaa gaaggtgaac ccgagctcgc ggaaacgatg
      721 gacgtgcgcg ggagaggcgg agtagatgcc caccgggagg tcgcggctcc ggcattcgtc
      781 gacgatctcg tgcaggaggc gctcgaatcc gggatcgtcg tactgggcgc ccagcggtag
      841 gccggcgctc agggccagat ccgacggccc gacgaacacc gcgtccagtc cctcgaccgc
      901 aacgatgtcg ggcagcgcct ggactgctgc gagggtctcg accatcacga tgcactgcgg
      961 gtccggtccg ccgggcgacc aggcccggcg cgtcgggccg tagctgcggc cgccccgcgg
     1021 cgggtagtgg cacgcggcga cggcccgcgc cgcgtcctgc gcggtgtcga ccaacggcac
     1081 gatcacgcct gctgcgccgc ggtccagcgc ccggccaatc gcgctcggct cgttgccggt
     1141 gacgcgtacc acgaccggcg tgccggtcgc ggccagcgcc tgggccatcg gaagcagggc
     1201 gtcgtcgccg atcaggccgt gctgctggtc gacgcagacg aagtcgaagc ccaccgagcc
     1261 catgagctcg gcggaggcgg aaccgggcag ctgtagccag ccacccacga ggtcggcggc
     1321 caggtcgagg gagggagcgt ccactgggtg aactctgcca ggcgctcgcc ccgaagggga
     1381 gttccgcgtg cagccggaac aatccgtgca tctgcttgct ttagcaacta tcatgccgcc
     1441 aggtgagggg agaacgagga tgagcgacat cgctccgcac ggactgcacc acgtcaccgc
     1501 gatcgcgtcg gacccgcaac ggaacgtgga cttctacacc cgggtcctgg gtctgcggct
     1561 ggtcaagcag acggtcaact tcgacgcgcc cgacacgtac cacctgtact acggagacac
     1621 gcagggcacg ccgtccacga tcctgacctt cttcccgtgg cagggtgtgc cctcgggacg
     1681 ccagggcagc ggcctgacca cggccaccgc gttcagcgtg ccgccggagt cgatgggctg
     1741 gtggcagcga cacctcacgg gcctcgggct cgagctcgat gggccgcgca cccgcgacgg
     1801 cgaggaggtg ctgaccctgc gcgaccccga cgggatggtg atcgacctcg tcgcggctgc
     1861 gggagacgcc cgctcgggct gggacggcgt ggcgggcgtg ccggccgagc acgcggtacg
     1921 gggactgcac gccgtgacca tgtcggagcg gctgccggag gccaccgcgg agctgctctc
     1981 cgcgacgctc gggatgcggc tgcagggcga ggacggcgac cgcacgcgct ttgcgatggg
     2041 ggcaggtggc accggcgcgg tggtcgacgt ggccgccgac tcgcgcccgc gtgggctgca
     2101 ggcgggcgga actgtgcacc acatcgcctt ccgcgccccc gaccaggcca cccaggcccg
     2161 ctggcggcag gagctcgtcg aggcggggct gcacgtcacc gagatcatcg accgccagta
     2221 cttcacctcc atctacttcc acgagccggg gggagtgttg ctggagatcg cgaccgacca
     2281 gccgggtttc accgtcgacg agccgctgct ggagctgggg cggtcgttga agctgccgcc
     2341 gtggttggag cccagccggg agcagatcgc ggcggcgctg ccgccgctgc gggtcgacga
     2401 gcccgtggag tcgtcatgag ggaagcagcg acgggtccga cgttgcagcg tccgcacgtc
     2461 tggctcccgg gcaccggcgg accgccactg ctgttcctgc acggaaccgg cggcgacgag
     2521 cacgacatgc tcccgctgcg tgggcacctt gcccctgacg ccccggtgct gtcgccgcgc
     2581 gggacggtgc tggagaacgg catgccgcgg ttcttccgga ggctccgtga gggcgtcttc
     2641 gacgaggacg acctgcgcga gcggaccgac gagctggcgg agttcctgac cgcggccggg
     2701 accgagcacg gggtggcccc gggatcgtgg gtggcggtcg gattctccaa cggcgcgaac
     2761 atcgcgtccg cgctgctgtt ccggcgcccc gaggcgctgg ccggcgccgt gttgctggcg
     2821 gcgatggtgc cgttcggcga cgtgccgggc ctcgatgtga acctgacggg taagcgcgtg
     2881 gccgtcgtca acggccgccg cgacccgatg gcgaccgcag cccagacgcg cacgctcgtc
     2941 gcgcagctgc gggaccgcgg cgcggcggtc gtcgagttcc cgcacgacgg cggtcactgg
     3001 atcgatgtgg cggttctccc tcggatccgg gagttccttg ccgctgggcc cgcgtgagtc
     3061 agcggggtca ctcgtcggcg tcgacgacgt gaccccgctg acggtcacgg aggtcgttca
     3121 gcccgctgcg ggcaccgccg ggatgccgtt cgccggtggg gtcgtgggcc ggctggtcga
     3181 ctgggtgatg ggggcgtcgc cgttgtccgt caccaggacc ggcgtgaggc tctcggtctt
     3241 gccgcccgtg atgcgggtga cctccatccc ggagatgccg gcgtgccggt cggcggagaa
     3301 ccggaacggc gcgacccacg gtccctggaa ctgggccccg gcccgctcga ccgcctggac
     3361 gacaccctgc cgggtcgggt cgggccccgc ggcctgcaac gcctgcacga aggtgtaggc
     3421 ctgggacatg ccgtagatgc ggtagttcgt cagctcgccg tcgccgccgt gctcatccca
     3481 cacgcgttgg aacatctgcg tccacgggtt gtccggcgtg tccacgccgg gcaggtactc
     3541 ggtcgtgatc acgccgtcga gcaggccggc gtccgtgacg gcgccctggg agaaccgggc
     3601 cagcagagac ccgacgagcc cggggtccga gccgacgttg gagtagatcc actgcgggcg
     3661 gtagttcagc cgcagggact gcagctgcga cagcgcggtg taggagggca cgttgaaccc
     3721 gatgacgagg tcggccccgg cggcctgcaa cgcggcgatc tgcggagcga cgtcggtgtt
     3781 gcccggcgtg taccgctcgg cggccacgat ctgggcgccg aggtaccggc gggcgccgag
     3841 ttcgccgtcc cggccgaagt cgtcgtcctg gaggaacagg ccgaccttgg cctgcgggaa
     3901 gttctgcgcc acgtactgcc cgacgatctt cccctcgatc tcgtagtcgg gctgccagcc
     3961 gaaggtctgc gggttcttgg cgggctggtc ccacagcagt gagccggagg agacgaacag
     4021 gtcgggcaca ccctcgctgt tgaggtagtc gagcacggcc gagtgcgtgg gcgtgccgag
     4081 cccactgagg agcgcgaaca cctggtcctg cagcaccagc tcgttgacga cctggctggt
     4141 ctgcgtcggg ttgtaggcgt cgtcgcggaa gacgtagtcg atcttccggc cattgacgcc
     4201 gcctgcggcg ttgacgaagt cgaagtaggc cttcgccccc gtcgggatct cgctgtagcc
     4261 gggcgccgcg acgccggtga gggggaagtg cgccccgatc ttcaccgacg tcgcggtgat
     4321 gccgacggtg gcaggaccgc cctcgccgcc agacccgccg tcaggacggc cgcctgcacc
     4381 gcacgcgctg aggaccagcg ctgccgcgac taccgccgcc gccagcgtcg agcgcctgct
     4441 tctcctgatc atcctgtcct cctcgtcgag ggaccccgcc gatttctcct gctccggttc
     4501 cacagcttct tcatcacgcc gacggctccc gacggtgcga gcagcatcac gagcacgagc
     4561 accacgccgt agacgaacgg cgcgagctgc gcggcctgcg tgccgtcgag cccggcgccg
     4621 ataccgatgt tggtgacggc gggcggtagg aacaccagta gcgcgctccc gagcagcgcg
     4681 cccacgaggc tgccgagccc gccgacgacc accgccgtga gtagccccag ggacagcacg
     4741 atggtgaatc cgctgggtgc agccaggcgc accacgatgc cgagtaccgc cccggccagg
     4801 ccggcgcagg ccgcgctgac gacgaacgcc agcacgcgca cccggcccag ccggatgccg
     4861 gccagctcgg cggccacctc gtcgtcgcgg acggcgcgcc agacccgccc ggtgcgtccg
     4921 gccatcaggt tggccagcag caggagcacc agcagcagcg tcccccagcc gacgtaggcg
     4981 aggtacttgg cagtgggcgt ctcgttggcg gagaggaagt agacggcgtc ggcgaaccag
     5041 ccgggcacgt ggggagaggt gacgggcaga ccctgctcgc cgcccagcgc cgtgtcgaag
     5101 tagatcgcga gacccggtac cgcgacggcc agcgccagcg tcgctccggc caggtagggc
     5161 ccgtgcagcc gggccgcggc cgccccgacg acggcgccga ccagcgcggt gactgcggcc
     5221 gcgaccagca tgacgacggg cagcggcagg cccccgtcgc cggtgagcag cagccccgtc
     5281 gtgtaggcgc ctacggccat cagggcgccg tggcccaggg agatctggcc gttgagtccg
     5341 gtcaggacgg tcagcccggc ggccgcgatg gcgtagtagg acatcgtcgc gaactgcgag
     5401 ttgcggaacg ggctcgtcag ttccagcacg agcaccacgg caacgagcgc ggcgagggtg
     5461 atcagcgcgt gccggccgaa cgtcgagcgg ggcatccggc ggcgcagcgg ggcgaagcgc
     5521 cggccgggag ctcccgccgc gggcgcctcg gccgcgggga cccgctcggc cgccggcgtg
     5581 ccggtcctgt cgatctgctg ggccatcaga ccctccgcgc cgcggtgcgg gcgaacaggc
     5641 cctgcggccg caccagcagc accgcgacga ggattgccag cgcggccagc gccaccaact
     5701 cgctgccgag gtagccgctg acgtaggaca gcagcagtcc gatggtcagc ccgccgacca
     5761 cggcgccgcc gggggagtcg agcccgccga gaaccgcggc gacgaacccg tagacgatca
     5821 ccgcgtccat gtaggcgggg aagaccaagc tgccgccggc gatgagcagc ccggacagcg
     5881 acccgaccac cgcggccagc gcccagccga gcgtgagcat ccgcccgacc ggcacgccga
     5941 gcagccgcga cacctcctgg ctgaacgcgg cggcccgcat cttcagcccg agatcggtga
     6001 agcggaacag cgccaccagc gcgagcatca ccaccacgac ggccgcgatg gtgtagatgc
     6061 tgttcgcggt gaacgcgatg ttgatcccgc ccactgcgaa cccgcgcagg tcgaacggtg
     6121 cagggaagga ccggaacgtg gagccgaaga ggatcgcggc cagcgcctgc agcgccacga
     6181 acagcccgag cgtgacgatc acggcgttga gctccgggcc gccctccacg ggccggatca
     6241 ccagtcgctc gacgacggcg cccagcacca gcccggtgac cagcgcgacg gcgagcccga
     6301 cccagtagga ctgcccaccc tcgatcacgg tgagcgccag gtaggtcgtg aacatggcca
     6361 tggcgccctg ggcgaagttg acgatgcggg tggaccgcca gatcagcacc agagcgagtg
     6421 caaaggccgc gtagaccgcc ccctgaccga gcccggtcag cgtgacgttg aggaactgct
     6481 gcactgctct cccctcatca agcgcgggcg attcagaagc ccaggtaggc gtggcgaagg
     6541 ccgtcgtcgg ccgcgagcgc cgccgcgctg tcccgcgcca ccacccggcc caggttgagc
     6601 acgatcccca cgtccgcgat ggagagggcg ctgcgcgcgt tctgctcgac gagcagcacg
     6661 gtgacgccgt cgctgtcgac gaggtcgcgc agcagaccga agatctgcgc gacgatccgg
     6721 ggtgccaggc caagcgacgg ctcgtcgagc agcaggacgt cggggccgga gagcagcgcg
     6781 cgtccgatga cgagcatctg ccgctcgccc ccggagagca cgtgcgcgtc gcggcggcgg
     6841 cgctcggcca gcggcgggaa catctcgtac acctgcgcca agctgcggcc cgcacggtcg
     6901 gactgcggca tcgatcccgc gcgtcgacgg gacgctccga gcagcgcgcc caggcgcagg
     6961 ttctcctcca ccgtgagctc ggtgatcacc ccgcggccct cgggcacgtg cgcgatcccg
     7021 gcgcgcgcga tcgcctcggc cggagcgcgg gtgaggtcgg tgccgtccag cagcacctgt
     7081 cccgcatccg gccggaccag gcccgagatg gtgcgcagca gcgtcgactt gccggccccg
     7141 ttggcgccga gcacggccgt gaccttcccg cgctcggcgg tgatgtccac ggcgtcgagc
     7201 gccgtgaccg ggccgtacgc cgtggtgagg ccccggacct ccagcgttcc cgtgcgcacc
     7261 gctgatgacg ggctcgcagg ctcgctcacg agtgcgtcgc ctcctcgccc aggtaggcgt
     7321 cgatcaccgc ggggtcgtcg cggacctcgt caggggttcc ctcggccacg accctgccga
     7381 agtcgagcac ggtgatccgg tcgcagaccg acatcacgag gtccatgtgg tgctccacca
     7441 gcaccaccga catccggtcg gtgagcgtcc ggatcagctc gccgagctcg gtcatctcgt
     7501 cgttgctcag cccgctggcc ggctcgtcga gcagcagcag gtcgggttcc gcggcgagcg
     7561 cgcgggccag cgcgacccgc ttctgcacgg ggtaggggag gctgccgggg agccggtcgg
     7621 cgtagccgtc cgcgccgagc tcgcagagcg cggccatcgc gcgctcgcgc agtgcccgct
     7681 cgtcccggtc ggagcgggcc agcgcgaaca gggtggcggc gaagcctgac ctgcggtgcc
     7741 ggtccgcacc caccatcacg ttctcggcca cggtgagccc cgtgaacagc ccgacgccct
     7801 gcagcgtgcg cgcgatgccc atcccggcga gccggtgcgg gcgcaggtcg cgcaccggtt
     7861 tcccgcggcg cagcaccgtg ccggtgtccg gacggacgaa gccgcagcag acgttgaaga
     7921 gggtggtctt gcccgccccg ttcgggccga tcaccccgtg cacctgaccg gccccgacgg
     7981 cgagggacac gtcgtcgagg gcgacgagcc cgccgaaccg gacgccgatc gcgctggctg
     8041 cgaagacggc ggtcgaaaga tccggctgtg ccggcggtcc gttcggtgat gtcggcccgt
     8101 tcggtgatgt cagcgctgac acgtgggcgt ctcccggggc tcgggcggcg gtgaccccga
     8161 tcaccggggg tgcccacact ttgggcgccg cgcccagcac gcaccatgtg cgcggcgccc
     8221 aagatcgtgg cccgaaaact gggcagagcg cccctggggg tggacggagc tgtgcgcgct
     8281 gttgcagagt gcgcggatgc tgcccaccgg gaggagcgcc cccggaaccc gtatcaacgc
     8341 tggcggagac cgtgtcgacg atgcagggag cgccggcgct gccccgacct acagcgcatc
     8401 ccgtgcgccc gcgcgcccgc tcccggacga gtcctccgag gcactgcggg tgctcggcgg
     8461 cgtgctcctc gacgaggtcg acgggttggc cgaccggctg acggtgatgg tgctgcagcg
     8521 cgagccggtg tacgccgaac tggacatggt ggaggagctg cgcggcgcct gccgggtgaa
     8581 cctggagcgc ggggtgcagg tgctcgccga gcaggtaccg ggcggggtgg accccgagga
     8641 cacctcgcgg cagaccggca ggcgccgcgc gcgccagggc gtgccgctgg aggtcgtcct
     8701 gcgcgcgtac cggctgggcg ggcgcgtcat gtgggaggcc ctgctcggcg cctcgcgcgg
     8761 ccggttctcc ggacggtacg acgacgtgct gctggacgct gcgagctacg tctggcgagt
     8821 caacgacagc agctcgtcgg cgctcgtcga ggcgtaccgg ctcgaggagc tgcggctgca
     8881 gagccacgac ctgagcaggc gtcacgccgt gctcgacggt ctcatcgagg gccgtgggag
     8941 tgatccggcg ttcctgcggg acgcggccac cgtcctcggg ctgcccgagc gcggtccggt
     9001 gctgtgcgtc gtggcgccgg tggagccgtc cggggaggac cccctgcggt caccgcagga
     9061 ggcgctgtcc gcgcacggcc tcgtctcgtc ctggttcctg cgcccacgcg acgaggtcgg
     9121 cgtggtggcg ctggcggcac ggccgcacga ggtggcgctc gacgcgctgc gcccgtgcgc
     9181 cgagggacgg gtgggcgtct ccccggtggt ggacgggctc ggggcgatcg acgcggggta
     9241 ccggatggcg cacaccgcgg cccgcacgct gtccgggccc ggtctggcgg tgctcgacga
     9301 gcggcttccc gaggcgctgc tggtggacag ccccgagctg gtgccccggg tcgtgcgtac
     9361 ggggctgggt agcctgctcg atctgcccgt cgttgaccgg gacatgctgc tggagacgtt
     9421 gcaggagctg ctggcacacg gtggctcgcc gacgtacgcc gcggcggcgc tgttctgcca
     9481 ccgcaacacg gtgatctacc ggatgcgccg gatcgaggcg gccaccgggc gctccatggg
     9541 ggatccccgg gaccggctgc tgctcaccct cggggtgatg gcggcacgca tgggtgcgtg
     9601 atcttgcggg gcccggtcac gacgcgacga gctcgcgttc cagtggcgtg cggaaccgcg
     9661 gcgtcacccg gacgtcgccg atccgcgccg cgagtcgctc ggccgccgcg tgcacggcgg
     9721 cggtcgcttc gctgccgacg tcctccagca gccgcagtgc aactgtgccg tccgcgcggt
     9781 gggcccagcc gcccacgatc cgcccgtcgc accagaccgt cgggccgatg ttgccgctgc
     9841 ggtcgaacag ggcggccttg tgcgggccga ggaaccaggc ccggtcctgc cagcccatcg
     9901 gcgtcgggtc cagcgcgggc agcaacgcca cccacggccc ggggtcgggc accggctcga
     9961 cgtcgtgggg cagcaccacc ccggggacgc cgtcgaggtc cacgggaacc gcacccagcg
    10021 cggcgagcgc ggcgcgggtg cgggccatcg tccagcccgt ccaccatttc agatcggcca
    10081 ctggcgccgg cccgaacacg gtgagccacc gccgcgccag ctcggttcgc gccgcaccct
    10141 gctccagctc aggcatgccg tccggccacc acgccgacac gggcgaccag cggtactgcg
    10201 tcgaggtcca accgccgaga gggcgaccgc gcacgatgtg accgtcggcg gagagcagca
    10261 gcatggcgcg gctcgtcatg ttctgcgcgg tgtcgtcggg cggtacggcg agccgcgtgc
    10321 gcaaccgcgg ctcgtcggcc acgagctcca cagccgtggc ctccccccgt tccagcagcg
    10381 ctttcaacgt ggacgcctcg acgtcggcga gccacgctgc cggctcgtcg gtcaccccgg
    10441 accgcccgat gtgctcgacg agcctgcggc gctggacagc ggcgacgtcg agcgtgcacg
    10501 cggcctgcac caccggtgcg acgtcgagcg ccaccaccca gaccgtccgc cgcatcgaca
    10561 gcatccgcac gagtgcgcgc tcgtcgtgca gcacccgctc gaccgtgggc acgtcggcgc
    10621 cggaccgggc gaggaccgcc agcacgacgg acgcggggtc ggtggcgtgc agcgcgacga
    10681 gcgcgtcggc cacgccgacc actccggcca ccgagcccgg cgcgcctgtt gcgggagcga
    10741 ggagatggcg ccgcccgagc cgtgcccgtc gctccgccgt accgatcgtc atcgccgcag
    10801 ccacggggag atgatggacc acctcgggta cggctcagcg cgcgaccggg caggcgaagg
    10861 ccgcccgtcc gccgcacgtc a
//
LOCUS       DASRSC010000326         6446 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5213395,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000326 DASRSC010000000
VERSION     DASRSC010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6446)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6446)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6446
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5213395"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="VFE39_15975"
     CDS             <1..550
                     /locus_tag="VFE39_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6260135.1"
                     /translation="RVVGQAGDGAALLAAVDAHRPDVVLADVQMPGIDGLAALRMLRA
                     RADAPPAAVLTTFDLDDYVAEALRIGVQGFLLKDAEPEALVRAVRDLAAGGAVLDPRI
                     TARLLPRLAAGSAAPRPAGLTLREQQVLRGLAAGDSNAGIASRLGLAEATVKKYVSAL
                     LTKLGAENRVQAALLAQGWGLN"
     gene            589..1749
                     /locus_tag="VFE39_15980"
     CDS             589..1749
                     /locus_tag="VFE39_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HET6260136.1"
                     /translation="MAGRRAYLPRAASELAVVLVPAVAVLWSEPPFSWSVPSALVACG
                     LLQLRHIAPPLAVVGGLWGLAGGLGWPAALVALYALGRRGGRVSTTIPWLVLPVVAAV
                     TPVLMTQALPWQRMVLTVAFVGLYTVVPASLGLLMATRERLTESLRELERARDEALAA
                     SQDAARAQERARIGREIHDAVGHHATLIAVGAAAIAASTGEDETRRGAEQLRALAKRA
                     LAEMRVALGLLDGREHAAGLTEIAALVAGSKAAGVDVELLNSGAPGEVGPAVGRAVYR
                     VVQESLTNAARHAAGAAVRVELRWNPLELQVQVQNRPPTRPSRWRRDPLGGGGAGLTG
                     LAERVASVGGQLSSGPTAADGFAVRATFPLRGQPAPAPAAAPAHEEQLPAVR"
     gene            complement(1756..2208)
                     /locus_tag="VFE39_15985"
     CDS             complement(1756..2208)
                     /locus_tag="VFE39_15985"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6260137.1"
                     /translation="MTTSHPSVAGPAALPRALPELLSQVAHQVSRRVETALGPDGPTL
                     DQWRVLALLTDGAGHPMTEIASHARVPAPTLTKIVDRLVDRALVYRRVDEGDRRRVLV
                     FLSEHGRTIHERLAPAVAGIGDALMDELGSHEAHRLVELLERLRELPR"
     gene            2372..3433
                     /locus_tag="VFE39_15990"
     CDS             2372..3433
                     /locus_tag="VFE39_15990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6260138.1"
                     /translation="MARDTLDVAFVVPRSGPAAIVGPSCEACGLLAADEVNADGGVLG
                     RELRLRVVDGGRSPGRVASEIDDLITAGTVEAVTGWHISAVRQQLAPIVSGRAPYVYS
                     PLYEGGERTPGVFLAGETPDRQLLPALRWMARERGVRTWCVVGDDYVWPLVSADVARR
                     YAHRCADIWILDEVFVPLGTQDFAAAVGRVERSGADGVLMLLVGSDAVRFNRAFATAG
                     LDAACVRLSPLMEENMVLATGVDATRELYAAAGFFEALATASGLDFSRRYAARFGVQA
                     PVLGSLGESCYEGVLLLARLIERAGGLGQAELALAAETLAYDSPRGTVQMAGNHLMQN
                     VYLARADGLELDVVAQLAP"
     gene            3490..3774
                     /locus_tag="VFE39_15995"
     CDS             3490..3774
                     /locus_tag="VFE39_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HET6260139.1"
                     /translation="MPTYGYRCPSCGSFELVRPMAEASDPAWCPDCGEVARRIFGGPA
                     LRRLDPGVRRALDAEVRSSDAPQVVTSVPGRSRRATPITTDPRHARLPRP"
     gene            3810..5057
                     /gene="fmdA"
                     /locus_tag="VFE39_16000"
     CDS             3810..5057
                     /gene="fmdA"
                     /locus_tag="VFE39_16000"
                     /EC_number="3.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014670338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004328 - formamidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formamidase"
                     /protein_id="HET6260140.1"
                     /translation="MPDVVFSIDQAKSMRDQRVPGHNRWHPDIPPAATVRPGQSIRVE
                     CREWTDAQIGNNDSANDVRDVDLTHAHMLSGPIFVEGAEPGDLLVVDILDLGPVPQEV
                     GDVPGQGWGYTGIFAKANGGGFLTDTFPDAYKAIWDFAGQKCTSRHIPGVEFTGITHP
                     GLFGTAPSPDLLARWNARERALIATDPDRVPPLALPPLEESTLGGTATGEALAAIGRD
                     GARTVPARENGGNHDIKNFTRGSRVFYPVHVPGALFSGGDLHFSQGDGEITFCGAIEM
                     GGFIDFHVDLIKGGMETYGVTTNPILMPGNVEPRYSEFVTFIGIGVDHTSDTQLYNDA
                     TVAYRNACLNAIEYLKKFGYSGAQAYLLLGAAPIEGRVSGVVDIPNACCSLYLPTAIF
                     DFDIRPSASGPVKADRGSCAVSS"
     gene            5054..6094
                     /locus_tag="VFE39_16005"
     CDS             5054..6094
                     /locus_tag="VFE39_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6260141.1"
                     /translation="MTGPDAPYDVHEIPDFTLECGATLRPAKVAYQTYGTLNAAKDNA
                     IVYPTWYSGRHWDNEWLIGEGLALDPERWFIIVPNMLGNGLSSSASNTPPPWDRARFP
                     QIAVRDNVASQHRLVTEVFGIETLPLVLGWSMGAGQTYQWAVSHPEMVQRIVPFCGSS
                     RTAPHNKVFLDSLRYALRADAAFAEGWYPPDAWPVRGLRAFARIYAGWGFSQAFYWDE
                     VWRELGYSSLEDFAVGFWEGFFLDDRDPNNLLTMLDTWWHGDVGTTPGFASTEAALAS
                     IRARALVMPAEKDLYFPPEDEQWAVSHMPNAELRVIPGVWGHFAGGGANPVDIAFIDQ
                     AVSELLAEPSGI"
     gene            6312..6385
                     /locus_tag="VFE39_16010"
     tRNA            6312..6385
                     /locus_tag="VFE39_16010"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6346..6348,aa:Pro,seq:ggg)
BASE COUNT          869 a         2231 c         2352 g          994 t
ORIGIN      
        1 ccgggtggtc gggcaggcgg gcgacggcgc cgcgctgctc gccgccgtcg acgctcaccg
       61 accggacgtc gtgctcgccg atgtccagat gcctgggatc gacgggctgg ccgcgctgcg
      121 gatgctccgc gcccgcgccg atgcgccgcc cgccgcggtt ctcaccacct tcgacctcga
      181 cgactacgtc gccgaggccc tgcggatcgg ggtgcagggc ttcctgctca aggacgccga
      241 gccggaggcg ctcgttcggg ccgtgcgtga tctcgcggcc ggtggcgccg tgctcgatcc
      301 gcgcatcaca gcgcggctgc tcccccgcct cgcggccggc tcggccgcgc cgcgacctgc
      361 cgggctgacc ctgcgcgagc agcaggttct gcgtgggctc gccgcgggcg attcgaatgc
      421 ggggatcgcc tcccggctcg gcctggccga ggccaccgtc aagaagtacg tgtcggcgct
      481 gctcaccaag ttgggtgcgg agaaccgcgt gcaggcagcc ctgctggcgc agggctgggg
      541 cctcaactga ggcgcaggtc ggcgggagga ccgagcgggg gagatcgggt ggcgggtcgg
      601 agggcgtatc tgccccgcgc tgcgtcggag ctggcggtcg tgctggtgcc cgcggtcgcg
      661 gtgctgtggt cggagccgcc gttctcctgg tcggtgccgt cggcactggt ggcctgcggg
      721 ctgctgcagc tgcgccacat cgcgcccccg ctcgccgtcg tgggtggcct gtggggcctg
      781 gcgggcggac tcggatggcc cgccgcgctg gtcgcgctct atgcactggg taggcgcggc
      841 ggacgcgtgt ccaccacgat tccctggctg gtcctgccgg tggtcgcggc tgtcacaccc
      901 gtgctgatga cccaggcgct gccctggcag cgcatggtgc tgaccgtagc gttcgtcggg
      961 ctgtacacgg tggtgcctgc ctcgctggga ctgctcatgg ccacccgcga acggctgacc
     1021 gagagcctgc gtgagctcga acgcgcgcgc gacgaggcgc ttgcggcctc gcaggacgcg
     1081 gcgcgggctc aggagcgcgc gcggatcggc cgggagatcc acgatgcggt cgggcaccac
     1141 gccacgttga tcgcggtcgg agcggcggcg attgccgcct ccaccggtga ggacgagacg
     1201 cgccgcgggg ccgagcagct ccgtgccctc gccaagcggg cgctggcgga gatgcgggtc
     1261 gcgctgggcc tgctcgacgg gcgcgagcac gccgccgggc tcaccgagat cgcggcgctg
     1321 gtggccgggt cgaaggcggc cggtgtcgac gtggagctgc tgaacagcgg cgcgccgggc
     1381 gaggtcggtc ctgccgtcgg acgggcggtg tatcgcgtgg tgcaggagtc gctcacgaat
     1441 gcggctcggc acgcggcggg tgcagcggta cgcgttgagt tgcgctggaa tccgctggag
     1501 ctgcaggtgc aggtgcagaa ccgacccccg acccgcccgt cccgctggcg gcgcgacccg
     1561 ttgggcggcg gcggagccgg cctgaccggc ctcgcggagc gcgttgcgtc ggtgggtggg
     1621 cagctgtcgt cgggtccgac ggccgccgac gggttcgccg tgcgggccac attcccgttg
     1681 cgcgggcagc cggcaccggc acccgccgcc gcaccggccc acgaggagca gctgccggcc
     1741 gtcaggtagc cggcgtcacc ggggcagttc gcgcaggcgt tccagcaact ccacgagccg
     1801 gtgggcctcg tggctgccca gctcgtccat cagcgcgtca ccgatgccgg cgaccgccgg
     1861 tgccagccgc tcgtggatgg tgcgtccgtg ctcggagagg aacaccagca cgcgccgccg
     1921 gtcgccctcg tccacccgcc ggtacaccag cgcgcggtcc accagccggt cgacgatctt
     1981 ggtcagggtg ggggcgggga cccttgcgtg actggcgatc tcggtcatgg ggtgacctgc
     2041 accgtcggtg aggagcgcca gcacccgcca ttggtcgagc gtgggcccgt cgggcccgag
     2101 cgcggtctca acccggcgtg agacctgatg cgccacctgg ctgagcagct ccgggagcgc
     2161 gcgggggagc gcggctggtc ccgccacgga cgggtgagaa gtggtcacgt catcctcctt
     2221 cggcgcgtga cgtcggcacc gacatcgtca acactatggt ctggcaggaa gtcagcccag
     2281 aggaagccag ctcagaggaa gccagcccag aggaagtcag ctcagaggaa gtcacctcga
     2341 accggacgac gtgcgggacc gggggtgcgc catggcacgg gatacgctcg acgtcgcttt
     2401 cgtggtgccg cgcagcggcc cggcggcgat cgtggggccg tcctgcgagg cgtgcggcct
     2461 gctggccgcc gacgaggtga acgcggacgg cggggtgctc ggccgcgagc tgcggcttcg
     2521 ggtcgtcgac ggggggcgct cgcccggcag ggtggcgagc gaaatcgatg acttgatcac
     2581 tgccggaacg gtcgaggcgg tcaccggctg gcacatctcg gctgtgcgtc agcagctcgc
     2641 gccgatcgtg tccgggcgcg cgccctacgt gtacagcccg ctctacgagg gcggcgagcg
     2701 gacaccgggg gtcttcctgg ccggcgagac cccggaccgg cagctgctcc cggcactgcg
     2761 ctggatggcc cgggagcggg gcgtacggac atggtgcgtc gtcggcgacg actatgtctg
     2821 gcctctcgtc tcggcggacg tggcgcggcg atacgcacac cgctgcgccg acatctggat
     2881 cctcgacgag gtcttcgtgc cgctgggtac ccaggacttc gcggcggccg tgggtcgggt
     2941 cgagcgcagc ggcgcggacg gtgtgctgat gctgctggtc ggctcggacg ccgtgcgctt
     3001 caaccgcgca ttcgccaccg cggggctcga cgccgcgtgc gtgcggctca gtcccctcat
     3061 ggaggagaac atggtgctcg ctacgggcgt cgacgccacc cgcgagctgt atgcggcggc
     3121 gggcttcttc gaggcgctgg ccaccgcgtc cggcctcgac ttctcccggc gttatgcggc
     3181 ccggttcggc gtgcaggcgc cggtgctcgg ctcgctcggt gagtcctgct acgagggcgt
     3241 tctgctgctg gcgcgactga tcgagcgggc aggcggcctc gggcaggcgg agctggctct
     3301 cgccgccgag acgctggcct acgacagccc tcgggggacg gtgcagatgg caggcaacca
     3361 cctgatgcag aacgtctacc tggcgcgcgc ggacgggctc gagctggacg tcgtcgctca
     3421 gcttgcgcct tgactggtca ttcctatcgc aatattttca tatgacagtt tctgatcagg
     3481 aggtgagccg tgccgaccta cggctaccgg tgcccctcgt gcggttcctt cgagctcgtc
     3541 cggccgatgg ccgaggcatc ggacccggcg tggtgtcccg actgcggcga ggtcgcacgt
     3601 cggatcttcg gcgggccggc gctgcgcagg ctcgatcccg gagtgcgccg tgcactcgac
     3661 gccgaggtgc gcagctccga cgccccgcag gtggtgacct ccgtgcccgg acgttcccga
     3721 cgggccaccc cgatcacgac cgatccccgg cacgcccgcc tgccccgacc ctgagctggc
     3781 ggtctcaact tccctccttg gaggtcccca tgcccgacgt cgtcttttcg atcgaccagg
     3841 ccaagtcgat gcgcgaccag cgggtgccag ggcacaaccg ttggcacccc gacattccgc
     3901 ccgcggccac cgtgcgaccc gggcagtcga tccgcgtcga gtgtcgtgaa tggacagacg
     3961 cccagatcgg caacaacgac tccgcgaacg acgtgcgtga cgtcgacctg acccacgcgc
     4021 acatgctgtc cggcccgatc ttcgtcgagg gtgccgagcc cggcgacctg ctcgtcgtcg
     4081 acatcctcga tctcggtccc gtaccgcagg aggtcggcga cgttcctggt cagggctggg
     4141 ggtacaccgg gatcttcgcc aaggccaacg gcggtggctt cctcaccgac accttcccgg
     4201 acgcgtacaa ggcgatctgg gacttcgccg ggcagaagtg cacgtcccgg cacatcccgg
     4261 gtgtcgagtt caccggcatc acccaccccg gcctcttcgg gaccgccccg tcacccgacc
     4321 tgctcgcccg ctggaacgct cgcgagcgcg cgctcatcgc gaccgatccc gaccgcgttc
     4381 cgccgctggc cctgccgccg ctggaggagt ccaccctcgg cggtaccgcc accggggaag
     4441 ccctcgccgc catcggccgc gacggcgccc gcacggtccc ggcccgggag aacggcggca
     4501 accacgacat caagaacttc acccgcggct cgcgcgtctt ctacccggtg cacgtgcccg
     4561 gtgcgctgtt ctccggcggg gacctgcact tcagccaggg cgacggcgag atcacgttct
     4621 gcggcgcgat cgagatgggc ggtttcatcg acttccacgt cgacctgatc aagggcggga
     4681 tggagaccta cggggtgacg accaacccca tcctcatgcc gggcaacgtc gagccgcgct
     4741 actcggagtt cgtgacgttc atcggcatcg gcgtcgacca cacgagcgac acccagctct
     4801 acaacgacgc caccgtcgcc tatcgcaatg cgtgtctgaa cgccatcgag tacctgaaga
     4861 agttcggata ctcgggtgcg caggcctacc tgttgctcgg agccgcgccc atcgagggcc
     4921 gggtcagcgg ggtggtggac atccccaacg cgtgctgctc gctttacctc ccgaccgcga
     4981 tcttcgactt cgacatccgg ccatcggcat cgggccccgt gaaggcggac cgggggagct
     5041 gcgcggtgtc atcatgaccg gacctgatgc cccgtacgac gtccacgaga ttcccgactt
     5101 caccctcgaa tgtggtgcga cgctgcgccc cgccaaggtg gcctaccaga cgtacgggac
     5161 gctcaacgcg gcgaaggaca acgccatcgt ctacccgacc tggtactcgg gccggcactg
     5221 ggacaacgag tggctgatcg gcgaggggct ggcgctcgac cccgaacgct ggttcatcat
     5281 cgtgccgaac atgctgggca acgggctgag ctcgtcggcg tcgaacacgc cgccgccgtg
     5341 ggaccgggcg cggttcccgc agatcgcggt gcgcgacaac gtggcgtcgc agcaccgcct
     5401 cgtcaccgag gtgttcggga tcgagacgct cccgctcgtg ctggggtggt cgatgggtgc
     5461 gggacagacc taccagtggg cggtcagcca cccggagatg gtgcaacgga tcgtgccgtt
     5521 ctgcgggtcc tcgcgtacag cgccgcacaa caaggtcttc ctcgactcgc tgcgctacgc
     5581 cctgcgcgcg gacgccgcgt tcgccgaggg ctggtaccca ccggacgcct ggccggtgcg
     5641 gggcctgcgg gcgttcgccc ggatctacgc cggctggggc ttctcccagg cgttctactg
     5701 ggacgaggtc tggcgtgagc tcgggtacag ctcactcgag gacttcgccg tcggcttctg
     5761 ggagggcttc ttcctcgacg accgcgaccc caacaacctg ctgacgatgc ttgacacctg
     5821 gtggcacggg gacgtcggca ccacgccggg tttcgcctcg accgaggctg cgctcgcctc
     5881 gatccgcgca cgcgccctcg tcatgcctgc ggagaaggac ctctacttcc cgccagagga
     5941 cgagcaatgg gcggtctcgc acatgccgaa cgcggagctg cgggtcatcc cgggcgtctg
     6001 gggccacttc gccggtggcg gagccaaccc ggtcgacatc gcgttcatcg accaggccgt
     6061 gtcggagctg ttggcggagc cgtcggggat ctgacggcta ccgaccggta cccacgaagg
     6121 tcgcccgcgc agggcgaccg agggcggtag tgcgctggac tcgtccggca caaactggcc
     6181 gggccgtgac ctcacgagag ccgcggggag tggcgggaag tggagctacc gggccagtgg
     6241 ggggagtcac gaccggccgg tgggggcgga tcagcggtgc ggtaggctga tcgagcctcc
     6301 tcgggggcgg ccgggacgtg gcgcagcttg gtagcgcact tgactggggg tcaaggggtc
     6361 gcaggttcaa atcctgtcgt cccgacggca cgatgggcgg ttccctttcg tcgggggagc
     6421 cgcccatcag cgtttctacc tgcgga
//
LOCUS       DASRSC010000327         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1287316,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000327 DASRSC010000000
VERSION     DASRSC010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1287316"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2287)
                     /locus_tag="VFE39_16015"
     CDS             complement(<1..2287)
                     /locus_tag="VFE39_16015"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HET6260142.1"
                     /translation="MTLEIGLLDVAVWELAQDIWTLTGLVGELPADLPRRAEVLRALE
                     RMVDVVDPDEVSGCAALGRAELASVLAAPAHASAHRVHAVGHAHIDSAWLWPVRETVR
                     KVARTFSNVLDLMDQDEEFVFAASSAQQYAWLQEHYPELFERVKARVAQGRFVPVGGM
                     WVESDTNMPGGEALVRQFVMGKRFFLEKLGVDPLEVWLPDSFGYSAALPQIIAGAGAR
                     WFLTQKISWNETNVMPHHTFRWEGIDGTQVFTHFPPVDTYNSELSAAELARAQRQYAE
                     KGRANTSLVPFGWGDGGGGPTREMLAAAARARDLEGSPTVRITTPAEFFATAEDEYPR
                     PPVWSGELYLEFHRGTYTSQTRTKRGNRRSEHLLREAELWATTAAVRTGAAYPADILE
                     RCWRTVLLQQFHDILPGTSIAWVHQEAERNYARVAAELEDVIARSLAALAGDGDREIV
                     VNAGPYARDGVPALGGAVRGYECSDTRIRARPRTGEDVTVTETADGVVLANSAVRVVV
                     DARGLLTSVRDLAADRELVPPGATANLLQLHRDTPAKWDAWDIDEHYRRTVRDLVDLD
                     ELAVVERGPSRAAVRIVRSTGSSTITQVVAVHAGSAEIDVVTDVDWHERQKLLKLAFP
                     LDVHADRATSEIQFGHVHRSTHANTSWDAARFETVAHRWVHVGEPGYGVAVANDSTYG
                     HDTLRATRPDGGTTTTVRLSLLRAPLFPDPTADQGAHRLAVALRVGSTIGDAVASGYR
                     LNLPLRTVRGAHAVAPLLAVDD"
BASE COUNT          323 a          825 c          843 g          318 t
ORIGIN      
        1 gatcgtcgac cgccagcagt ggggcgacgg cgtgggcgcc gcggaccgtg cggagcggca
       61 ggttgagccg ataacccgag gccactgcgt cgccgatcgt cgaaccaacc ctcagcgcga
      121 cggcaagccg gtgcgcaccc tggtcggcgg tggggtcggg gaacagcggc gcgcgcagca
      181 gcgacagccg cacggtggtc gtcgtgccgc cgtcgggacg ggtcgcgcgg agggtgtcgt
      241 ggccgtaggt ggagtcgttg gccacggcca ccccgtatcc cggctcgccc acgtgcaccc
      301 agcggtgcgc gaccgtctcg aaccgcgccg cgtcccacga tgtgttggcg tgggtcgagc
      361 ggtgcacgtg gccgaactgg atctcggacg tggcgcggtc cgcgtgcacg tcgagcggga
      421 acgcgagctt gagcagcttc tgcctctcgt gccagtcgac gtccgtgacg acgtcgatct
      481 ccgcagagcc tgcgtgcacg gcgacgacct gggtgatcgt ggacgaaccg gtcgaccgga
      541 cgatccggac cgcggcgcgg gacgggcccc gctcgaccac cgccagctcg tcgaggtcga
      601 cgaggtcgcg gacggtgcgc cggtagtgct cgtcgatgtc ccaggcgtcc cacttggccg
      661 gcgtgtcgcg gtgcagctgc agcaggttcg ccgtggcgcc gggcggcacg agctcccggt
      721 cggcggcgag gtcgcgcacg gaggtcagca ggccgcgggc gtcgacgaca acccggacgg
      781 cggagttggc cagcaccacg ccgtccgccg tctccgtcac agtgacgtcc tcgccggtgc
      841 ggggccgtgc gcggatacga gtgtcagagc actcgtatcc acgcacggct ccgccgaggg
      901 ccgggacgcc gtcgcgggcg taggggccgg cgttgaccac gatctcccgg tcgccgtcgc
      961 ccgcaagcgc cgccagcgac cgcgcaatca cgtcctccag ctccgcggcg accctggcgt
     1021 agtttcgctc ggcctcctgg tgcacccagg cgatggacgt gccaggcaga atgtcgtgga
     1081 actgctgcag cagcacggtg cgccagcagc gctccaggat gtcggcgggg taggcagcgc
     1141 cggtgcgcac ggcggcggtg gtggcccaca gctcagcctc ccgcagcagg tgctcgctgc
     1201 ggcggttgcc ccgcttcgta cgcgtctgcg aggtgtacgt gcctcggtgg aactccaggt
     1261 acagctcgcc cgaccacacg ggcggtcgcg ggtactcgtc ctcggcggtg gcgaagaact
     1321 cggcgggcgt ggtgatccgc accgtcggcg agccctccag gtcgcgcgca cgggcggccg
     1381 cggccagcat ctcgcgggtc ggtccgccgc cgccgtcgcc ccagccgaac ggcaccagcg
     1441 aggtgttggc cctccccttc tcggcgtact ggcgctgcgc ccgggccagt tcggcggcgg
     1501 acagctcgga gttgtaggtg tcgaccggcg ggaagtgggt gaagacctgc gtgccgtcga
     1561 tgccctccca gcggaaggtg tggtgcggca tcacgttggt ctcgttccac gagatcttct
     1621 gcgtgaggaa ccagcgggcg cccgccccgg cgatgatctg cggcagcgcc gccgagtagc
     1681 cgaaggagtc gggcagccac acctccagcg gatccacgcc cagcttctcg aggaagaacc
     1741 gcttgcccat gacgaactgg cgcaccagcg cctccccgcc cggcatgttg gtgtccgact
     1801 ccacccacat cccgccgacg ggcacgaacc ggccctgcgc cacccgggcc ttgacccgct
     1861 cgaacagctc cgggtagtgc tcctgcagcc aggcgtactg ctgcgcggac gaggccgcga
     1921 acacgaactc ctcgtcctgg tccatcaggt cgagcacgtt ggagaaggtg cgtgccacct
     1981 tgcggacggt ctcccggacc ggccagagcc aggcggagtc gatgtgggca tgccccaccg
     2041 catgcacgcg gtgcgcgctg gcgtgggccg gagcggcgag caccgacgcg agctcggccc
     2101 gcccgagtgc ggcgcagccg gacacctcgt cggggtccac gacgtccacc atgcgctcca
     2161 gggcccgcag cacctcggcg cgtcgcggca ggtcggccgg gagctcgccg accagcccgg
     2221 tgagcgtcca gatgtcctgt gccagctccc acaccgcgac gtccagcagc ccgatctcca
     2281 gcgtcacgag ccggtagagc ggatccgag
//
LOCUS       DASRSC010000328         6225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4562274,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000328 DASRSC010000000
VERSION     DASRSC010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6225
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4562274"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VFE39_16020"
     CDS             complement(<1..455)
                     /locus_tag="VFE39_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET6260143.1"
                     /translation="MCPIVDRARAGLAELADPVAAAAMQRYMKSAMPFRGVAKPARER
                     LLRTLVDECPFPDAPALRDAVRELWGGARFREERYLALALVDHRRYRQWPDPSWVPLL
                     REWIVAGAWWDFTDEIASRRIGPLLRAHPVALTPVLRLWSTDPDRWLRRT"
     gene            complement(577..1548)
                     /locus_tag="VFE39_16025"
     CDS             complement(577..1548)
                     /locus_tag="VFE39_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HET6260144.1"
                     /translation="MANTSSRTLRLLSLLQTHRYWPGAELADRLEVSVRTLRRDIDRL
                     RELGYPVEASRGVDGGYQLAPGAALPPLVLDDEEAVALAVGLQTAAQGGVAGIAESSV
                     RALAKVVQVMPPRLRHRVDALRAMTVPAGWGSSGPSVDPGDLTVLAQACRDTERLEFG
                     YTSASGERTERHVEPLRLVPLGRRWYLVAYDLTRHDWRSFRLDRLDRPRSTAARFRPR
                     ELPAEDAAAFVRAGIGQVPAPHTVEVLVEAPAAVVREQVGRWGTVEDAGDGRCLLRMK
                     SDSLDWPTMALGGLGADFTVLSPPELLDHVRSWARTFSRATGPTGHD"
     gene            1644..2195
                     /locus_tag="VFE39_16030"
     CDS             1644..2195
                     /locus_tag="VFE39_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HET6260145.1"
                     /translation="MTTTTTTAGATVTGERADLLQTLAQHRYFLRHTVQGLTDEQARQ
                     RTTASELCLGGLIKHLTDVERKWVDFILEGPSAMGGGWEGGVPPQEVIDSWPANFRLL
                     PEETLAGALAEYEAVARRTDELVSTLPDLDASHPLPEAPWFEKGARWSHRRVLLHIMA
                     ETAQHAGHADIIRESMDGQKTMG"
     gene            2247..3272
                     /gene="argC"
                     /locus_tag="VFE39_16035"
     CDS             2247..3272
                     /gene="argC"
                     /locus_tag="VFE39_16035"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HET6260146.1"
                     /translation="MHVLRIAVAGASGYAGGELLRLLLAHPEVEIGALTAGGSAGTPL
                     GAHQPHLLPLAGRILVDTTPETLAGHDVVFLALPHGRSAELAAQLGDDTVVIDCGADH
                     RLTDGAAWDRWYGGEHAGSWPYGLPELPGARDALRGTTRVAVPGCYPTAVTLALAPAL
                     AAGLVTPDVVVTAVTGTSGAGKSLKPHLLGAEVMGSLSAYGVGGVHRHTPEMTQNLSG
                     VAGTPVVVSFTPVLAPLPRGILASCSAPLAGDGADAREVYEKAYTDEPFVHLLPEGTW
                     PTTAATLGANTAHLQVAVDPDAQRLVAVAAIDNLTKGTAGGAVQCMNLAVGLPETTGL
                     PLTGVAP"
     gene            3380..4489
                     /gene="argJ"
                     /locus_tag="VFE39_16040"
     CDS             3380..4489
                     /gene="argJ"
                     /locus_tag="VFE39_16040"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HET6260147.1"
                     /translation="MKASGALDLALVVNDGPEAGAAGVFTRNKVKAAPVLWSQQVLTS
                     GRLRAVVLNSGGANACTGPEGFQTAHATAEKAAELLGCGAVEVAVCSTGLIGKQLPRD
                     VVLAGVEKATAGLDATAEAGLAAATAVMTTDTVAKQAAHVDPAAWSIGGFTKGAGMIA
                     PSMATMLTVLTTDAVLDPAELEPALRAAVRVSFDRLDIDGSMSTNDTVLLLASGASGV
                     TPAPDEFDAALTSVCQDLARQMQADAEGVTKRITVQVTGAADEDDAVTVARTVARDSL
                     VKTAIFGSDANWGRIAAAAGYADADVDPDRLDITINGSLLCRGGVAAGGRTDANLSGP
                     DVLVAIELGIGSGTAEIRTTDLSYAYVEENSAYPS"
     gene            4521..5393
                     /gene="argB"
                     /locus_tag="VFE39_16045"
     CDS             4521..5393
                     /gene="argB"
                     /locus_tag="VFE39_16045"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HET6260148.1"
                     /translation="MRLKRAGEKAGVLAEALPWLQRFHDQVVVVKYGGNAMIDEELKQ
                     AFAQDMVFLRLAGIHPVVVHGGGPQITAMLDRLGLPAEFRGGLRVTTPETIDIVRMVL
                     VGQVGRELVGLVNQHGPYAVGLSGEDAGLFTAEKRTALVGGEPVDIGLVGDVVHVNPD
                     AVLDIVRAGRIPVVAGVAPDIDGTVYNINADSAAAALAAALGAAKLVVLTDVEGLYAN
                     YPDPESVISALRAEELEPMLPTLESGMAPKMEACLRAVRGGVPQAHVIDGRVPHSVLL
                     EVFTSEGVGTMVVP"
     gene            5467..>6225
                     /locus_tag="VFE39_16050"
     CDS             5467..>6225
                     /locus_tag="VFE39_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET6260149.1"
                     /translation="MSEELTTRWKTAMMDNYGTPPLALVRGSGAEVWDADGRRYLDMV
                     GGIAVNALGHAHPAVVEAVTRQISTLGHTSNLYITQPPLDLAEKLLELLDQPAARVLF
                     CNSGAEANEVAFKMARRTGRPKMIAAENAFHGRTMGALALTGQAPKRTPFEPMTPGVS
                     HVPYGDVAALDAAVGPDTAAVFLEPILGEAGAVNPPAGYLEAAREITATRGALLVLDE
                     VQTGIGRTGAWFAHQRNGIVPDVVTLAKGLGGGLP"
BASE COUNT          910 a         2229 c         2258 g          828 t
ORIGIN      
        1 gtgcgacgca gccagcggtc cgggtcggtg gaccagagcc gcagcacggg cgtcagcgct
       61 accgggtgcg cccgcagcag cgggccgatc cgcctgctcg cgatctcgtc ggtgaagtcc
      121 caccacgccc ccgcgacgat ccattcccgg agcagcggca cccacgacgg gtcgggccac
      181 tgccggtatc ggcggtgatc gacgagggcg agcgcgaggt agcgctcctc gcggaagcgc
      241 gccccgcccc acagctcccg cacggcgtcc cgcagtgcag gggcgtcggg gaacgggcac
      301 tcgtcaacca gcgtgcggag cagccgttcc cgcgcaggct tcgcgacacc tcggaacggc
      361 atcgcggact tcatgtaccg ctgcatcgcc gccgcggcga ccggatcggc cagctccgcg
      421 aggcccgcgc gggcacgatc gacgatcgga cacacgccgg gaaccgtacg tcgaatcacc
      481 caccgatcgc cgtgccggta cacgccctgc acgcggcgtg cggcctgccg gacaggtgtc
      541 tgggcgcgcg ccccgagccg cgccgggtca gtcacgtcag tcgtgtccag tcgggccggt
      601 ggcccggctg aaggttcggg cccagctgcg gacgtggtcg agcagctcgg gaggggagag
      661 caccgtgaag tccgcaccca gcccgcccag cgccatcgtg ggccagtcca gcgagtccga
      721 cttcatccgc agcaggcacc ggccgtcccc ggcgtcctcg acggtccccc agcgcccgac
      781 ctgctcacgg acgaccgccg ccggggcctc gaccagaacc tcgacggtgt gcggggccgg
      841 gacctggccg atgcccgccc gcacgaacgc cgccgcgtcc tccgcgggga gctcgcgcgg
      901 ccggaaccgg gcggcggtgc tgcgcggccg gtcgaggcgg tcgaggcgga agctgcgcca
      961 gtcgtgccgg gtgaggtcgt aggccacgag gtaccagcgg cgcccgagtg ggacgagccg
     1021 cagcggctcc acatggcgct cagtccgctc gccgctcgcc gaggtgtagc cgaactcgag
     1081 ccgctccgtg tcccggcagg cctgggcgag gacggtgagg tcgccggggt ccacgctcgg
     1141 gccggagctc ccccaccccg caggcaccgt catcgcgcgc agggcgtcca cccggtgccg
     1201 cagccgcggt ggcatcacct gcaccacctt ggccagagct cgcaccgacg actccgcgat
     1261 ccccgcgacc ccgccctgcg cggccgtctg cagcccgacg gcgagggcga ccgcctcctc
     1321 gtcgtcgagc accagcggtg ggagcgcagc acccggcgcc agctggtagc cgccgtcgac
     1381 cccgcggctc gcttccacgg gatagcccag ctcgcgcagc cggtcgatgt cccgacgcaa
     1441 cgttcgcacg gagacctcga gccggtccgc cagctctgcg cccggccagt accggtgggt
     1501 ctgcagcagc gagagcaacc gcagcgtccg cgagctcgtg ttggccatgt ttccgattct
     1561 cccgcaattg aggtcagaaa ctggcacttg ggcttcataa cgtactcctc gacggggcga
     1621 ccgatccgga aggcggagag acgatgacga cgacaaccac cactgcgggt gcgaccgtga
     1681 ccggagagcg agccgatctg ctgcagacgc ttgcgcagca ccgctacttc ctgcgccaca
     1741 ccgtgcaggg cctgacggac gagcaggccc ggcagcgcac cacggccagc gagctgtgcc
     1801 tgggcggcct gatcaagcac ctcaccgacg tcgagcggaa atgggtcgac ttcatcctcg
     1861 agggcccgtc cgcgatgggc gggggctggg agggcggcgt gcctccgcag gaggtcatcg
     1921 actcgtggcc ggcgaacttc cgcctgctcc ccgaggagac cctcgccggc gcgctcgccg
     1981 agtacgaggc cgtggcacgg cgcaccgacg aactggtgag cacgctcccc gacctcgacg
     2041 cctcccaccc gctgcccgag gccccgtggt tcgagaaggg tgcgcgctgg tcgcaccggc
     2101 gggtgttgtt gcacatcatg gcggagaccg cccagcacgc tgggcacgcc gacatcatcc
     2161 gcgagtccat ggacgggcag aagaccatgg ggtgatcggc gtcgcgcgct ttttgtttgc
     2221 atcgagttgc gctctcgtgc atagtcatgc acgtgcttcg gatcgcagtg gcaggtgcca
     2281 gcgggtacgc agggggggag ctgctccggc tcctcctcgc gcatcccgag gtggagatcg
     2341 gcgcgctgac ggcgggcggg agtgcgggca cgccgctggg cgcgcaccag ccgcacctgt
     2401 tgccgctggc cggtcgcatc ctcgtcgaca cgacgccgga gacgttagcc ggacatgacg
     2461 tcgtcttcct ggcgctgccg cacgggcgct ccgcggagct ggcggcgcag ctgggtgacg
     2521 acacggtggt tatcgactgc ggcgccgacc accggctcac ggacggggcc gcgtgggacc
     2581 gctggtacgg cggtgagcac gccgggagct ggccctacgg gttgcctgag ctgcccggcg
     2641 cccgggacgc gctgcgtggc accacccggg tggccgtccc gggttgctat cccacagcgg
     2701 tcaccctcgc gctggccccg gcgctggcag caggcctcgt cacgcccgac gtcgtcgtca
     2761 ccgccgtcac cgggacctcc ggcgccggga agtcgctcaa gccgcacctg ctcggtgccg
     2821 aggtgatggg ctccctctcg gcgtacgggg tgggcggcgt gcaccggcac acgccggaga
     2881 tgacgcagaa cctgtcgggc gtagcgggca ccccggtggt cgtgagcttc acgcccgtcc
     2941 tcgccccgct tccgcgcggg atcctggcca gctgctccgc gccgctggcc ggtgacggcg
     3001 ctgacgcccg cgaggtgtac gagaaggcct acaccgacga gccgttcgtg cacctgctcc
     3061 ccgagggcac ctggcccacc acggcggcga cgctcggtgc caacaccgcc catctacagg
     3121 tcgccgtcga tcccgacgcg cagcggctgg tcgccgtcgc cgcgatcgac aatctcacga
     3181 agggcaccgc cggaggcgcg gtgcagtgca tgaacctggc cgtcgggctg ccggagacca
     3241 ccggcctgcc gctgacgggg gtggctccgt gagtgagctt gcgaacgaac catcagcgcc
     3301 gagtgagctt ccgagccagc agtcgagaga gggcgttgtt tcgccgaagg ggttccgggc
     3361 ggcgggggtc gccgccggga tcaaagccag cggagctctc gacctcgcgc tcgtcgtcaa
     3421 cgacgggccg gaggccgggg ccgcgggcgt gttcacccgg aacaaggtca aggccgcgcc
     3481 ggtgctgtgg tcccagcagg tgctcaccag cgggcggctg cgcgctgtcg tgctcaactc
     3541 gggcggggcc aatgcgtgca ccggtccgga gggcttccag actgcgcacg ccaccgcgga
     3601 gaaggcggcc gagctgctgg gctgcggcgc ggtggaggtc gcggtgtgct ccaccgggct
     3661 gatcggcaag cagctgccgc gggacgtggt gctggccggc gtcgagaagg ccaccgcagg
     3721 gctggacgcc accgccgaag ccggactggc cgccgccacc gccgtgatga ccaccgacac
     3781 cgtcgcgaag caggccgccc acgtcgaccc ggccgcctgg agcatcggcg ggttcaccaa
     3841 gggcgcgggc atgatcgcgc cgtcgatggc cacgatgctg accgtgctga ccaccgacgc
     3901 ggtgctcgac ccggccgagc tcgagcccgc gctgcgtgcc gcagtccgcg tcagcttcga
     3961 ccgcctggac atcgacggct cgatgtccac caacgacacc gtgctgctgc tcgcgtcagg
     4021 tgcgtcggga gtgacgcctg cgccggatga gttcgacgcc gcgctgacgt cggtgtgcca
     4081 ggacctcgca cggcagatgc aggccgacgc agagggcgtc accaagcgga tcaccgtgca
     4141 ggtcaccggt gcggccgacg aggacgacgc cgtgacggtc gcccggacgg tggcccggga
     4201 cagcctggtc aagacggcga tcttcggctc ggacgccaac tggggccgca tcgccgccgc
     4261 cgccggctac gccgatgccg acgtcgaccc ggaccgcctc gacatcacga tcaacggctc
     4321 cctgctgtgc cggggcggtg tcgcggcagg cggacgcacg gacgcgaatc tgtcgggtcc
     4381 ggatgtcctc gtcgcgatcg agctcggcat cggcagcggc accgccgaga tccgcaccac
     4441 cgacctgtcg tacgcctacg tggaggagaa cagtgcctat ccctcctgag gcgaacgccg
     4501 acgggaccgg ctccaccgcc ctgcgcctga agcgggcggg ggagaaggcc ggcgtgctgg
     4561 ccgaggcact gccgtggctg cagcgcttcc acgaccaggt ggtcgtcgtg aagtacggcg
     4621 gcaacgcgat gatcgacgag gagctcaagc aggcgttcgc gcaggacatg gtgttcctgc
     4681 ggctcgccgg catccatccg gtcgtggtgc acggcggggg gccgcagatc accgcgatgc
     4741 tcgatcggct cgggttgccg gccgagttcc gtggcggcct tcgcgtcacc acgccggaga
     4801 cgatcgacat cgtccggatg gtgctggtcg ggcaggtcgg tcgtgagctc gtcgggctgg
     4861 tcaaccagca cggcccgtac gctgtggggc tgtcgggaga ggacgccggg ctcttcaccg
     4921 cggagaagcg caccgcgctc gtcggtgggg aacccgtcga catcgggctg gtcggtgacg
     4981 tcgtccacgt caacccggac gcggtgctcg acatcgtccg ggccgggcgc atcccggttg
     5041 tcgcgggcgt cgcccccgac atcgacggca cggtctacaa catcaacgcc gacagcgcag
     5101 cagccgcgct cgccgccgcc ctgggcgccg cgaagctcgt cgtcctcacc gacgtcgagg
     5161 ggttgtacgc gaactacccc gacccggagt cggtgatcag cgcgctcagg gccgaggagc
     5221 tggagccgat gctgccgacg ctggagagtg ggatggcgcc caagatggag gcctgcctgc
     5281 gggcggtgcg cggcggcgtg ccgcaggccc acgtgatcga cgggcgggtg ccgcactcgg
     5341 tgctgctcga ggtgttcacc agcgagggag tcggaacgat ggtggtcccg taatgagcga
     5401 gcttgcgagc gaatcatcga tacagcgcct ccgcgcagcg ctgaccgagc gtagcgaggg
     5461 agtgcgatga gcgaggagct gaccacgcgg tggaagaccg cgatgatgga caactacggg
     5521 acgccgccgc tcgcgctcgt gcgcggaagc ggcgccgagg tgtgggacgc cgacggccgc
     5581 cgctacctcg acatggtcgg cgggatcgcc gtcaacgcgc tgggtcacgc gcaccccgct
     5641 gtcgtcgagg cggtgacgcg gcagatctcc acgctcgggc acacgagcaa cctctacatc
     5701 acccagcctc cgctcgatct ggccgagaag ctcctggaac tgctcgacca gcccgcggcc
     5761 agggtcttgt tctgcaactc cggcgcggag gcgaacgagg tggcgttcaa gatggcgcgc
     5821 cgcaccggcc ggccgaagat gatcgccgcc gagaacgcct tccacggtcg cacgatgggt
     5881 gcgctcgcac tcaccggcca ggcgccgaag cgcaccccgt tcgagccgat gaccccgggc
     5941 gtctcgcatg tgccctacgg cgacgttgcc gcgctcgacg cggccgtcgg cccggacacc
     6001 gccgcagtgt tcctggagcc gatcctgggc gaggccggcg cggtgaaccc gcctgcgggc
     6061 tacctcgagg cggcccgtga gatcaccgcc acccgcgggg cactgctcgt cctggacgag
     6121 gtgcagaccg gcatcggtcg gaccggcgcc tggttcgcgc accagcgcaa tgggatcgtc
     6181 cccgacgtcg tgacgctggc gaaggggctc ggcggcggtc tcccc
//
LOCUS       DASRSC010000329         5861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5050161,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000329 DASRSC010000000
VERSION     DASRSC010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5861
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5050161"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..256
                     /locus_tag="VFE39_16055"
     CDS             <1..256
                     /locus_tag="VFE39_16055"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HET6260150.1"
                     /translation="PAARQAIELVGSAGHLARALALGVIGFLAGTAALFADPARAGGL
                     DAALRTLSGTALGSSLLVVVAAGFAAFGLFCLADAATRRA"
     gene            382..1572
                     /locus_tag="VFE39_16060"
     CDS             382..1572
                     /locus_tag="VFE39_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6260151.1"
                     /translation="MDPVRNPFAPGAGQRPPELAGRDREVDAFEIVLERVARGRPERS
                     LVLTGLRGVGKTVLLGELRSMAVRAGWGAGKIEARPEADLRRPLSAALHRAIRDLAVR
                     HRAPDRVDEVLGILKAFALRSAPDGAKLRDRWQPGIDVPVKAGRADSGDIEIDLVELF
                     TEVAELAADVGTGVAILIDEMQDLRPDDISALCAACHELSQSRAPLVVVGAGLPHLPA
                     VLSASKSYSERLFKYVRIGQLDRPDADFALLAPAEREDASFDTDALDALFDASSGYPY
                     FVQAYGKAAWDAAPRSPISAADVKLAAPEAEAELAVGFFGSRYERATPAEREYLRAMA
                     ELADGRDEPVATAGIAEHLARRASSLSPARDSLLKKGLVFSAQRGQIAFTVPHFGRYL
                     LTQG"
     gene            1782..2099
                     /locus_tag="VFE39_16065"
     CDS             1782..2099
                     /locus_tag="VFE39_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET6260152.1"
                     /translation="MQDPKLEIQMLHDRVMLKPETTGERRSGAGIVIPATAEVAKRLV
                     WGEVFGVGANVRTVKVGDRVLFARDDQYEVDVQGATYLVLRERELHAVATERTEHGTG
                     LYL"
     gene            2152..4791
                     /locus_tag="VFE39_16070"
     CDS             2152..4791
                     /locus_tag="VFE39_16070"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HET6260153.1"
                     /translation="MPERQSSPGEVTARPETDVTGNDSSRGSAAQERPTDSAGGTEDV
                     AVAEQVAAQVAGSPPATEPGPVEPTAVAPTGDEPVLDVAAAVEMAQPGAEVSAGAAAP
                     PQPAPSARPAEQAQPGPLRQRPTPARPASSARPRPRPRPAPEGDESPTVRTAVGSSAA
                     AEDESPTVRTTVGSPAPGTPGPGSTADRARAGGAPAHGAAATGAPASARMAPAQGQAP
                     TVAVRAAGASAAAPPARPAGRTSASTAWFNPVDNESTQLIPRTPVAPAGPPAPAADPP
                     TERLSITRVTASPDATATSVGTAAPATSGPPGLPPTDGPDADRPEAGKSPPRRRRLLL
                     LTGVVGTLAVLYVGDLVLSSGSVPRGVSVVGVQVGGLGLDEAEQRLRGEIEPRTREPI
                     QVSVGEARSEIDPAAAGLGVDWDATLAVAGSQPLNPITRIASFFTHHEIGVVTTKNDD
                     SLTAALEELSPIVDKAPVEGSVRFEGATPVPVDPVAGQQLDVPAAAGVLQRDWASGRL
                     VALPLNELPPSTTPEDVVAAIEQVARPAVSAPVRMIGEGGAQGTVEPEVIASALTFRA
                     DGDRLVPEINPNAVTDALKPQLASSEVPGRDASLDFSSGGPVVVPSQDGRGVDYTASL
                     ASLLDVLTGTGPRQITAVYADQPAELTTEELNGLGITGVIGEFTTGGFAADSGRNIKR
                     GAEQINGMIVKPGETFSLNGATEPRDASHGYVEAGIISDGHASRGIGGGVSQVATTLY
                     NAAYFAGMVDVAHKEHSFYISRYPAGREATVFEGSIDLKFRNDNPTGVLIQTVWTPSS
                     LTVRLYGTKVYDVTSTPGPRTKPTSPKTITIPAGEPCSPSQGAPGFTVTDTRTLRNVS
                     TGESRNETRTVRYNPSPIVACGG"
     gene            4895..5320
                     /locus_tag="VFE39_16075"
     CDS             4895..5320
                     /locus_tag="VFE39_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET6260154.1"
                     /translation="MRIADVLRSKGTNVATVDPTATVAELIGELASHNVGALPVVEGG
                     QLVGIVSERDVVRRLHADGPGLLEARVGDIMIPGVTTCAPGDSVSDIAAVMTNRRFRH
                     LPVVVDGKLAGIVSIGDLVKARIDLLESEREQLQSYIAG"
     gene            complement(5377..>5861)
                     /locus_tag="VFE39_16080"
     CDS             complement(5377..>5861)
                     /locus_tag="VFE39_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HET6260155.1"
                     /translation="IERDNGQALAHVAGEIQAFLDAPDLMGTAGNGRLKEERALLGVA
                     LADVQGMLGALTGYLMASAEDPSSIYKAGQHTVRLLMAVGDLLIGWLLLRQAEVALIA
                     LGEDGPATTGASKDGAFYEGKVAVARFFAKTVLPQLTAQRVVVETADNSLMELAEAAF
                     "
BASE COUNT          864 a         2110 c         2055 g          832 t
ORIGIN      
        1 ccccgccgcc cggcaggcga tcgagctcgt cgggtcggcc gggcacctgg cgcgggcact
       61 cgccctcggc gtgatcggtt tcctcgccgg aactgccgcg ctgttcgccg acccggcacg
      121 cgccggtgga ctcgacgccg cgctgcgcac gctgagcggc accgcgctgg gttcgtcatt
      181 gctggtggtg gtggcggccg ggttcgccgc gttcgggttg ttctgcctgg ccgacgcggc
      241 cacccggcgc gcctgatcgg gctttctctt gctttctagc gattcaccga gatagaggaa
      301 tagcatccga tctgcgacac gccatcgggg ttgctagccg cttctacggt gggcacaaga
      361 ttcgccgata ccgtccgatg cgtggatccc gtgcgcaacc cgttcgcccc cggtgccggc
      421 cagcgacctc cagagctcgc ggggcgcgac cgtgaggtcg atgcgttcga gatcgtgctc
      481 gagcgggttg cgcgcgggcg cccggagcgc agcctggtgc tcaccggtct gcgcggcgtc
      541 gggaagaccg tgctgctcgg cgagctgcgc tccatggccg tccgcgcggg gtggggagcc
      601 ggcaagatcg aggcgcggcc cgaggcggat ctgcgcaggc cactgtcggc tgccctgcac
      661 cgggcgatcc gggacctcgc cgtgcggcac cgtgctcccg accgcgtgga cgaggtgctg
      721 gggatcctca aggcgttcgc cctgcgttca gcgccggacg gcgccaagct gcgcgaccgc
      781 tggcagcccg gcatcgacgt gcccgtcaag gcagggcgcg ccgactccgg ggacatcgag
      841 atcgacctgg tcgagctgtt caccgaggtg gccgagctgg cagcggacgt cggcaccggc
      901 gtcgcgatcc tcatcgacga gatgcaggat ctgcggcccg acgacatctc ggcgctctgc
      961 gcggcctgcc acgagttgtc ccagtcccgc gcgccgctcg tggtggtggg tgccgggttg
     1021 ccgcacctgc cggcggtgct ctcggcgtcc aagtcctact cggagcgcct gttcaagtac
     1081 gtgcggatcg gccagctcga ccggccggac gcagacttcg cgctgctcgc gccggccgaa
     1141 cgcgaggacg cctcgttcga caccgatgcc ctcgatgccc tcttcgacgc ctccagcggc
     1201 tacccctact tcgtccaggc ctacggcaag gccgcatggg acgccgctcc gcgcagcccg
     1261 atcagcgcgg cggacgtcaa gctggcggcc ccggaggccg aggccgagct cgccgtcggc
     1321 ttcttcggct cccgctatga gcgggccacc ccggccgagc gggagtatct gcgggccatg
     1381 gccgaactcg ccgacggccg cgacgagccc gtggccaccg ccgggatagc cgagcacctc
     1441 gcgcgccggg cctcgtccct gtcacctgct cgcgacagcc tgctgaagaa gggtctggtg
     1501 ttctccgcgc agcgtgggca gatcgccttc acggtgccgc atttcgggcg ctaccttctc
     1561 acgcaagggt gaagtcatag cccgaatggg tgaaacatga tgctagtgct tggcgaagta
     1621 acgggtgctc gacccgcttc gataggactt acagccccgc agtgctgtcg gacccccgac
     1681 ctgacagcac cgcggggcct tctcgttccc ggcccgctgc cggcccgacg aacgacgagg
     1741 tcaggaacgg gacgatcagg ggaggccgta gcatctggac ggtgcaggac cccaagctcg
     1801 agatccagat gctgcacgac cgcgtgatgc tgaagccgga aaccacaggc gagcgccgca
     1861 gcggcgctgg catcgtgatc ccggcaaccg cggaggtcgc caaacgtctt gtctggggag
     1921 aggtgttcgg cgtgggggcg aatgtccgca cggtgaaggt cggcgaccgg gtgctgttcg
     1981 cccgcgacga ccagtacgag gtcgacgtgc agggcgctac gtacctggtt ctgcgggaac
     2041 gtgagctgca cgccgtcgcc acagagcgca ccgagcacgg cacggggttg tacctgtgac
     2101 gaaccgggtc cgctgaaggc ccggtccgag ttgtgagggg aaacggactt catgcccgag
     2161 cggcagtcct cgcccggcga ggtgacggcc cgtccggaga cggatgtgac gggaaacgac
     2221 agcagccggg gatcggcggc gcaggagcgc ccgaccgact ccgcaggggg gacagaagac
     2281 gtcgcggtgg ccgagcaggt ggccgcacag gtcgcgggat cgccgccggc aaccgagccc
     2341 ggtccggtcg aacccaccgc cgtcgcgccc accggtgacg agcccgtcct ggacgttgct
     2401 gctgccgtcg agatggcgca acccggggcc gaggtgagcg caggtgcagc ggcaccgcct
     2461 cagccggctc catctgcacg cccggcggag caggcgcagc ccggtccact gcggcagcga
     2521 cccaccccgg cgcgaccggc gtcatcggca cgaccccgcc cgcgcccccg gccggcgccc
     2581 gagggcgacg agagcccgac cgtccgcacc gccgtcggca gctctgccgc cgcggaggac
     2641 gagagcccga cggtccgcac gacggtcggt tccccagctc cgggtacccc gggtcccgga
     2701 tccacggccg accgggcccg cgcagggggc gccccggccc atggggccgc agcgaccggc
     2761 gccccggcgt cggcccgcat ggctccggcc caggggcagg cgccgactgt cgccgtgcgc
     2821 gctgccggtg cgtccgctgc ggcgccaccg gcccgccccg ccgggcgaac cagcgcgtca
     2881 acggcctggt tcaacccggt cgacaacgag tccacccagt tgatcccgcg cacgcccgtc
     2941 gcgccggccg gccctccggc gccggcagca gacccaccca ctgaacgcct ctccatcacc
     3001 cgcgtcaccg catctcctga cgccacggcc acgtcggtcg gcaccgcagc gccggcgacg
     3061 tccggccctc cggggctccc gcccaccgac ggccccgatg ccgaccggcc ggaggccggt
     3121 aagagtcctc cgcgcaggcg caggctcctc ctgctcacgg gggtggtcgg cacactggcc
     3181 gtgctctacg tcggcgacct ggttctcagc tccggatcgg tgccgcgcgg cgtcagcgtg
     3241 gtcggggtgc aggtgggcgg cctcggcctg gacgaggccg agcagcggct gcgcggggag
     3301 atcgaaccgc gcacgcgcga gccgatccag gtcagcgtgg gtgaggcacg cagcgagatc
     3361 gatcccgccg ctgcgggcct cggcgtcgac tgggacgcga cgttggccgt ggccggttcc
     3421 cagccgctca acccgatcac ccggattgcg tcgttcttca cgcatcacga gatcggcgtg
     3481 gtcaccacga agaacgacga ctcgctcacc gcggcgctcg aggagctgtc gccgattgtc
     3541 gacaaggctc ccgtcgaggg gtcggtgcgg ttcgagggcg ccacgccggt tccggtcgac
     3601 ccggtggcgg gccagcagct cgacgtgccg gcagcggccg gggtgctcca gcgcgattgg
     3661 gcctccggac ggctggtagc cctgccgctc aacgagctgc cgccgtccac cacgccggag
     3721 gacgtcgtcg ccgcgatcga gcaggtcgca cggcccgccg tgtcggcgcc ggtcaggatg
     3781 atcggtgagg gcggggccca gggcaccgtc gagccggagg tcatcgcctc ggcactgacg
     3841 ttccgcgccg acggcgaccg gctggtcccg gagatcaacc cgaatgccgt caccgatgcg
     3901 ctgaagccgc agttggcgtc atcagaggtt ccgggtcgcg acgcgtcact cgatttctcc
     3961 tcaggtggcc ccgtcgtcgt gccgtcacag gacggccgcg gtgtcgacta cacggccagc
     4021 ctcgcgagcc tgctcgacgt gctcaccggc accgggccgc gccagatcac ggccgtctac
     4081 gcagaccagc ccgccgagct caccaccgag gagctgaacg ggctcggcat caccggcgtg
     4141 atcggcgagt tcaccaccgg tggcttcgcc gccgactccg gccggaacat caagcgcggt
     4201 gcggagcaga tcaacgggat gatcgtcaag cccggggaga cgttcagcct caacggcgcc
     4261 actgagcccc gcgatgcgag ccacggctac gtggaagccg gcatcatctc cgacggccac
     4321 gcttcgcgcg gcatcggtgg cggcgtctca caggtggcca ccacgctgta caacgcggcg
     4381 tacttcgccg ggatggtcga cgtggcgcac aaggagcaca gcttctacat cagccggtac
     4441 ccggccggtc gtgaggccac cgtcttcgag ggttccatcg atctgaagtt ccgcaacgac
     4501 aaccccacgg gcgtgctgat ccagacggtg tggacgccga gttcgctgac cgtgcggctc
     4561 tacggcacga aggtctacga cgtcacgtcg acgccgggcc cgcgcaccaa gccgacgagt
     4621 ccgaaaacga tcacgatccc ggccggagag ccgtgcagcc cgagccaggg cgcacctggt
     4681 ttcaccgtca ccgacacccg cacgcttcgc aacgtcagca ccggcgagtc gcgcaacgag
     4741 actcggaccg tccggtacaa cccgtctccg attgttgcgt gcggagggtg agcgccggcg
     4801 tacctcagta acccactcgt ggctcgggtc ttccgcgaaa cgggtcacag ttcgtactct
     4861 cggtccaatg gcgccaaagg atcgagggtt ggacatgcga atcgcggacg tgctgcggag
     4921 caagggcacc aacgtggcca cagtcgaccc cacggccact gtcgccgagt tgatcggtga
     4981 gctggcctcc cacaatgtcg gggcgctgcc cgtcgtcgag ggcgggcagc tggtggggat
     5041 cgtctccgag cgcgacgtcg tgcgcaggct gcacgccgac ggaccggggc tgctcgaggc
     5101 cagggtcggg gacatcatga tccccggtgt caccacctgt gcgcccggcg acagcgtgtc
     5161 cgacatcgcc gctgtgatga ccaaccgccg gttccggcac ctgcccgtgg tggtcgacgg
     5221 caagctggcc ggcatcgtca gcatcggcga cctggtcaag gcgaggatcg acctgctcga
     5281 gtccgagcgc gagcagctgc agagctacat cgccggctga cgaaacgaac ggcgccctcg
     5341 tccggaccca ccggacgaga gcgccgtcgg tgctgctcag aatgctgctt ccgccagctc
     5401 catcagtgag ttgtccgccg tctcgaccac cacgcgctgg gcggtgagct gcgggagcac
     5461 cgtcttggcg aagaaccggg cgacggctac cttgccctcg tagaaggccc cgtccttgga
     5521 tgcgccggtc gtggccgggc catcctcgcc gagcgcgatg agcgccacct cggcctggcg
     5581 tagcagcagc catccgatca gcaggtcgcc gacggccatc agcagccgca cggtgtgctg
     5641 cccggccttg tagatgctcg acgggtcctc ggcggaggcc atcaggtagc ccgtgagcgc
     5701 ccccaacatg ccctgcacgt cggcgagggc cacgccgagc agcgcgcgct cctccttcaa
     5761 ccggccgttg cctgctgtgc ccatgaggtc cggggcgtcg aggaatgcct ggatctcgcc
     5821 ggccacgtgg gcgagcgcct gcccgttgtc ccgctcgatc t
//
LOCUS       DASRSC010000330         5123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4397297,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000330 DASRSC010000000
VERSION     DASRSC010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4397297"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VFE39_16085"
     CDS             <1..926
                     /locus_tag="VFE39_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6260156.1"
                     /translation="AKEQTLEVLADGVYALTADGDPNIGAVEGEDFLVCFEALATPVA
                     ARKWLARLREHTDKPVRYLVLSHYHAVRVLGASAFDAEVIVAHENTRALVAERGKEDW
                     ESEFARMPRLADGADSVPGLTWPTLTFSDRMTIDLGGDRGDLVLQYCGRGHTEGDIVA
                     WLPRHRILFAGDLVEAEAALYTGDAFHRDWAGATLDRVAAMGAEMLVGGRGGVSHGAP
                     AVGSAIAQTRHFLQVLITEVGAVQQHGGTLKEAFERSHGALHERYGRWPIFEHCLPFD
                     VSRLWDELSGVERPIIWTAERDQEVWDQLQS"
     gene            923..2557
                     /locus_tag="VFE39_16090"
     CDS             923..2557
                     /locus_tag="VFE39_16090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HET6260157.1"
                     /translation="MNRVVVIGNGPVGQTAALLLARWGVATVVLDQRPVRDRVGSKAI
                     CQQRDVLDVWDAVDAGARIAAEGITWTTARTFHRGAELFSHTFVDAGCSPFPPFVNIS
                     QSRIEEILDECIGAQPLIEVRWGRRVTSLLQDADGVVLACDDGTQVQASFVVACTGAR
                     CDELRAQLGISFDGHSFDDRFLICDIRADLPEWATERHFHFDPAWNPGRQVLLHPCPD
                     STFRIDWQLPSDYDLDVDENSGALHRRIRAVIGDRPYEVLWKSVYRFQSRCVDRMRVG
                     RVLLAGDAAHLVSPFGARGLNSGVADAENAAWKIAFVVQGWAPVDLLESYHHERHAAA
                     RENLEITTATMDFLTPRSAAQVAHRHHVLSRAGHDPAARALVDSGRLAEPFWYVDSPL
                     TTPSPARPFIGRPPRGTVPPAGPGILVPDAPVLVRGDEGSGTRIHARRFRQLAREGLL
                     LLVAPGADAAAAKAATQGAEAPVRVLELAAIDQTGALGAALDARPGDVWMVRPDAHVA
                     AVLEHPTSAIIQTALHRALATTDREEDDHGVLPTVR"
     gene            2532..3731
                     /locus_tag="VFE39_16095"
     CDS             2532..3731
                     /locus_tag="VFE39_16095"
                     /EC_number="1.13.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004411 - homogentisate 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase domain-containing
                     protein"
                     /protein_id="HET6260158.1"
                     /translation="MAYYRRSGEVPPKRHTQHRTPDGGLYYEELMGEEGFSSDSALLY
                     HRGIPSAIVDASPWELPAVDTTPNHPLVPRHLKLHELFPGEDWKAVDAVTGRRLVLGN
                     ADVRISYVVAGEASPLYRNAIGDECVYVESGAATVETVFGTLDARQGDYVLLPRATTH
                     RWLPVGAAPLRLYVIEAASHITPPGRYLSRFGQLLESAPYCERDLHGPAEPLLVDGTD
                     VEVLVKHRGSQGITGTRYVCPTHPFDVVGWDGCMYPYTFNVNDFEPITGRVHQPPPVH
                     QVFEGAGFVICNFVPRKVDYHPLSIPVPYYHSNVDSDEVMFYCGGSYEARKGSGIGQG
                     SISLHPGGHSHGPQPGSYERSIGVQQVDELAVMVDTFRPLELGEGGRAAEDSGYAWTW
                     SGRGPRS"
     gene            3728..4927
                     /gene="fahA"
                     /locus_tag="VFE39_16100"
     CDS             3728..4927
                     /gene="fahA"
                     /locus_tag="VFE39_16100"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005438083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HET6260159.1"
                     /translation="MIELPGSTLFGPANLPYGVFSVPGDRRRRIGVAIGDHVLDVAPL
                     ADASDCGAELARLLAAPVLNPLLAAGRPTWTELRARLTDWIHGPADVLRPHLLAVEEV
                     ELHLPVDVGDYVDFYASEHHATNVGRIFRPGSEPLTPNWKHLPIGYHGRSGTIVVSGT
                     DIVRPQGQRRGDAGPEFGPSTRLDIEAEVGFVLGGATRMGESVAVADVAAHVFGVFLL
                     NDWSARDIQAWESTPLGPFLGKSFATSVSPWVVPLDALDAARVAPPPRDPPLLPYLRD
                     VDDRGLDLELEVALNGYVVSRPPFAGMYWTAAQQLAHLTVNGASVRPGDVLASGTVSG
                     PEKEQRGSLLELSWGGTEPIPLPAGDTRTFLQDGDEITITATAPGTAGSRIGLGEVRG
                     RVLPARP"
BASE COUNT          687 a         1828 c         1853 g          755 t
ORIGIN      
        1 ccgcgaagga gcagaccctc gaggtgctcg ccgacggcgt ctacgcgctg acggcggacg
       61 gcgaccccaa catcggtgcc gtcgagggtg aggacttcct cgtctgcttc gaggcgctcg
      121 ccactcccgt cgcggcgcgg aaatggctgg cccggctacg cgagcacacc gacaagccgg
      181 tgcgctacct cgtgctcagc cactatcacg ccgtgcgggt gctcggtgcc tccgcgttcg
      241 acgccgaggt gatcgtcgcc cacgagaaca ccagggcgct ggtggcggag cggggcaagg
      301 aggactggga gagcgagttc gcgcggatgc cgcgcctcgc cgacggcgcc gactccgtac
      361 ccggtctcac ctggcccacc ctcaccttct ccgaccgcat gacgatcgac ctgggcggtg
      421 accggggtga cctggtgctg cagtactgcg ggcgcgggca caccgagggc gacatcgtgg
      481 cctggctgcc gcggcaccgg atcctgttcg ccggcgatct cgtggaggcc gaggcagcgc
      541 tctacaccgg cgacgcgttc caccgcgact gggccggcgc gacgctggac cgggtggcgg
      601 cgatgggggc cgagatgctg gtcggcgggc gcggcggcgt gagccacggg gcccctgccg
      661 tcgggtcggc cattgcccag acgcggcatt tcctgcaggt gctgatcacc gaggtaggag
      721 ccgtgcagca gcacggcggc acgctgaagg aggcgttcga gcgcagccac ggcgcgctgc
      781 acgagcggta cgggcggtgg ccgatcttcg agcactgcct gcccttcgac gtctcccgcc
      841 tctgggacga gctgtcgggc gtcgagcgtc cgatcatctg gacggcggaa cgcgaccagg
      901 aggtctggga ccagctccag tcatgaaccg ggtcgtcgtc atcgggaacg ggccggtcgg
      961 tcagaccgcc gcattgctgc tcgcccggtg gggcgtggcg acggtggtgc tcgaccagcg
     1021 gcccgtccgg gaccgcgtcg gctcgaaggc gatctgtcag cagcgcgatg tgctcgacgt
     1081 ctgggatgcc gtcgatgccg gagctcggat tgcggccgag ggcatcacct ggaccactgc
     1141 tcggaccttc caccgtggtg cggagctgtt cagccacacc ttcgtcgacg caggctgctc
     1201 accgttcccg ccgttcgtga acatctcgca gtcgcgcatc gaggagatcc tcgacgagtg
     1261 catcggtgcg cagccactga tcgaggtgcg gtgggggagg cgggtcacct cgctcctgca
     1321 ggacgccgac ggggtggtgc tggcttgtga cgacggcacg caggtgcagg cgtcgttcgt
     1381 ggtcgcgtgc actggggcgc ggtgcgacga gctgcgcgcc cagctcggta tcagcttcga
     1441 cggccactcc ttcgacgatc gcttcctcat ctgcgacatc cgtgccgacc tgcccgaatg
     1501 ggctaccgag cggcacttcc acttcgaccc cgcatggaac ccgggccggc aggtgctgct
     1561 gcatccctgc ccggactcga cgttccgcat cgactggcaa ctgccctctg actacgacct
     1621 ggacgtcgac gagaactctg gcgcgctgca ccggcgcatc cgtgcggtga tcggcgaccg
     1681 gccgtacgag gtcctctgga agtcggtgta ccgcttccag tcgcgttgcg tcgaccggat
     1741 gcgcgtcggg cgggtgctgc tggccgggga cgctgcgcac ctggtgtcgc cgttcggtgc
     1801 gcgcggcctc aactccgggg tggcggacgc tgagaacgcc gcctggaaga tcgcgttcgt
     1861 ggttcagggc tgggccccgg tggacctgct ggagagctac caccacgagc ggcacgccgc
     1921 ggcgcgggag aacctcgaga tcaccacggc gacgatggac ttcctcaccc cgcggagcgc
     1981 tgcgcaggtg gcgcaccgcc accacgtgct ctcccgcgcc ggtcacgatc ccgccgctcg
     2041 cgccctcgtc gactccgggc ggctcgcgga gccgttctgg tacgtcgact cgccgctcac
     2101 cacgccgagc cccgcccgtc cgttcatcgg caggccgccc cgggggacgg tgccgcccgc
     2161 ggggcccggg atcctggtac ccgatgcgcc ggtgctcgtg cgcggagacg agggctctgg
     2221 cactcgtatc cacgcacggc ggttccgaca gctcgcgcgc gaggggctcc tactgctggt
     2281 cgcaccgggc gccgacgccg ccgcggcgaa ggcggccacg cagggtgccg aagccccggt
     2341 gcgggtcctg gagctcgcgg cgatcgacca gaccggcgcg ctcggtgccg cgctggatgc
     2401 gcggccgggg gacgtctgga tggtgcgacc ggacgcgcac gtcgccgccg tgctcgagca
     2461 tccgacgtcg gccatcatcc agactgcgct gcatcgcgcg ctggccacca ccgaccgtga
     2521 ggaggacgac catggcgtac taccgacggt ccggtgaggt gccgcccaag cggcacacgc
     2581 agcaccggac gcccgacgga gggctctact acgaggagct gatgggggag gagggcttct
     2641 cgtccgactc cgcgttgctc taccaccgcg gcatcccgtc ggcgatcgtc gacgcgagcc
     2701 cgtgggagct gcccgccgtc gacaccacgc cgaaccaccc gctggtcccg cggcacctca
     2761 agctgcacga gctgttcccc ggcgaggact ggaaggccgt ggacgccgtc acggggcgca
     2821 ggctcgtgct cggcaacgcc gacgtccgga tctcctatgt cgtggctggc gaggcgtcgc
     2881 cgctctaccg caacgcgatc ggtgacgagt gcgtctacgt cgagtcgggg gcggccaccg
     2941 tcgagacggt gttcggcacg ctcgacgcgc ggcagggcga ctacgtgctg ctgccgcggg
     3001 cgacgacgca ccgctggctg cccgtcggcg ccgcaccgct gcgcctgtac gtgatcgagg
     3061 cggcctcgca catcaccccg ccagggcgct acctctcccg cttcggtcag ctgctggaga
     3121 gcgcgcccta ctgcgagcgt gacctgcacg gtccggccga gccgctgctc gtcgacggca
     3181 cagacgtcga ggtgctggtg aagcaccgcg gcagccaggg gatcaccggc acccgttacg
     3241 tctgtccgac gcacccgttc gacgtcgtcg gctgggacgg ctgcatgtac ccctacacgt
     3301 tcaacgtcaa cgacttcgag ccgatcaccg gccgggtgca ccagcctccg ccggtgcacc
     3361 aggtgttcga gggcgccggt ttcgtgatct gcaacttcgt gccgcgcaag gtcgactacc
     3421 acccgctgtc gatcccggtg ccgtactacc actccaatgt ggactcggac gaggtgatgt
     3481 tctactgcgg cgggagctac gaggcccgca agggatcggg catcgggcag ggttcgatct
     3541 cgctgcaccc cggcggtcac agtcacggtc cgcagccggg atcgtacgag cgcagcatcg
     3601 gggtgcagca ggtcgacgag ctcgccgtca tggtcgacac gttccggccg ctggagctcg
     3661 gcgagggcgg gcgggccgcc gaggactcgg gctatgcctg gacctggtcg ggacggggac
     3721 cccgttcgtg atcgaactcc ccggcagcac gctgttcggc ccggcgaacc tgccctacgg
     3781 ggtgttctcc gtgccgggcg accggcggcg gcggatcggc gtcgcgatcg gtgaccacgt
     3841 gctcgacgtc gccccgctcg cggatgcgtc ggactgcggc gctgagctgg cgcggctgct
     3901 cgccgcgccg gtgctcaacc cgctgctcgc agccggtcgc cccacgtgga ccgagctgcg
     3961 tgcccgcctc accgactgga tccacggccc cgccgacgtg ctgcggccgc acctgctggc
     4021 ggtcgaggag gtggagctgc acctgcccgt cgacgtcggg gactacgtcg acttctacgc
     4081 cagcgagcac cacgccacga acgtcggccg tatcttccgg cccggctcgg agccgctcac
     4141 gccgaactgg aaacacctcc cgatcggcta ccacggacgc agcggcacca tcgtcgtgtc
     4201 cggcaccgac atcgtccgtc cacagggaca gcgccggggc gacgccgggc cggagttcgg
     4261 tccgtcgacg cggctggaca tcgaggccga ggtcggcttc gtcctcggcg gggccacgcg
     4321 gatgggggag tcggtcgcgg tcgccgacgt cgccgcgcac gtgttcgggg tgttcctgct
     4381 caacgactgg tccgcacgcg acatccaggc ctgggagtcc acgccgctcg ggccgttcct
     4441 cggcaagtcc ttcgccacgt ccgtctcgcc gtgggtggtg ccgctggacg cgctcgacgc
     4501 ggcgcgggtc gccccgccgc cgcgcgaccc gccactgctg ccctacctgc gcgacgtcga
     4561 cgaccgtggg ctggacctcg agctggaggt ggccctcaac ggttacgtcg tgtcccggcc
     4621 gccgttcgcc ggtatgtact ggacggcggc gcagcagctc gcgcacctga ccgtcaacgg
     4681 ggcgtcggtg cgtccgggcg acgttctcgc gagcgggacc gtctccggcc cggagaaaga
     4741 gcagcgcggc tcgctgctcg agctgagctg gggcggcacc gagccgatcc cgctgcccgc
     4801 cggcgacacc cgcacgttcc tccaggacgg cgacgagatc acgatcaccg ccacggcccc
     4861 cgggacggcc ggtagccgca tcggcctggg cgaggtccgc ggccgcgtcc tgccggcccg
     4921 cccctgaccg cgcgaccccg gcgcctccgc agcgtggctg tggacgggca ggagagcggc
     4981 ggctcagggc tgaaccgggc aactcagggg cgggatatgg gcgactcgcg cgggaggggc
     5041 gactcgcggg ttgacgacgg cgggtcgctt gcgggatccg ggcggcgtcc cgcgggacgg
     5101 gcgtctcgcg gcgactcgcg ggc
//
LOCUS       DASRSC010000331         8128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5867983,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000331 DASRSC010000000
VERSION     DASRSC010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8128
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5867983"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1296)
                     /locus_tag="VFE39_16105"
     CDS             complement(37..1296)
                     /locus_tag="VFE39_16105"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6260160.1"
                     /translation="MLLVEATSAALVVLGLLGQAPPTPAQLQVAGVLCLLGIAHTEVA
                     VGVERVRRRVTEGNHVDLSSVWTFAAAVALPASYAALVAVIVHCHLWWRAWRLRVPLY
                     RQIFSSATVVLACLASATVLERAGGPAEAGSNRQLWALALALLVYTTVNSCLVAGAIA
                     VSTPQAELSKVFAPWDENVLEIATLCLGAITAIAIGINPWLVLLVLPPLLVLHRAVLV
                     RQLEEAASTDGKTGLLNAAAWHAQAERALRRSNRHDGPRGVLVLDLDHFKAVNDTHGH
                     LAGDQVLAAVADAMRTEVRDRDLVGRFGGEEFVVLLAGLEGSGSVELEAVAERIRRRV
                     ANLRVEIPTPDGPLTVAGLTVSVGVAVSPAEGGDLRTLLQIADTALYAAKRAGRNVVR
                     LGLALPSQHPTPTQLPTAAGDPPAATG"
     gene            complement(2524..3789)
                     /locus_tag="VFE39_16110"
     CDS             complement(2524..3789)
                     /locus_tag="VFE39_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6260161.1"
                     /translation="MADGALGAVFRVREFRVVWAAELLSIAGDQLARVALAVLVYGRT
                     GSAAWAAATYALTFLPALLGGVLLAGLADRYRRREVMIASDVVRAVLVGAMAIPQLPL
                     WALCALVVVVVLLGSPHTAAQGALLPEILHGELYERGLAVRQITSQSAQLVGFASGGV
                     LVAAISPSMALLGNAASFSLSAVLVRAGLADRPRPGATEGGDAAAGADAPVGGLRGAV
                     AALFEIAADPRRRALVLLAWLVGWYVVPEGLAAPYADQLGAGPAVVGVLMAADPLGSV
                     IGAWLFVRFVPAQTRARIVGVLAVGAGLPLVLCLFRPAVPLTLVLWAVSGMLSTAYLL
                     QTQASFVRATPVVGRGRAIGVAASGIIAAQGAAVLIGGVLADEWDPSTAIAVAGAVGA
                     LLSLGAAVAWHRANRHHREPADTTVLASG"
     gene            complement(3889..5076)
                     /gene="ald"
                     /locus_tag="VFE39_16115"
     CDS             complement(3889..5076)
                     /gene="ald"
                     /locus_tag="VFE39_16115"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005163704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HET6260162.1"
                     /translation="MLVGVPREVKNHEYRVAITPAGVQELTSRGHEVLVERDAGAGAG
                     LPDDDFRAAGARTSADPDEVWGAAELLLKVKEPVPQEYHRLRADQVLFTYLHLAASKE
                     CTDALVTAGTTAVAYETVRLPDGSLPLLAPMSEVAGRMAPQVGAHHLQRTAGGRGVLL
                     GGVSGVPAGKVAVIGAGVSGMNAASIAVGMQAEVMVLDTNINRLREIDFTYRGHLQTI
                     TSNAYEVERACLWADLVIGAVLVPGAKAPTLVSNDIVSRMRPGSVLVDIAIDQGGCFA
                     DSRPTTHDDPVFGVHESIFYCVANMPGAVPNTSTRALTNATLPYVLALAGRGLAGAVG
                     DDPALAAGVNVAAGHIVQPEVAAAHGMPAVPLQDVLGDLPGPGRERPVPARVANLPPR
                     GAR"
     gene            complement(5091..5729)
                     /locus_tag="VFE39_16120"
     CDS             complement(5091..5729)
                     /locus_tag="VFE39_16120"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET6260163.1"
                     /translation="MQLVLVRHALPERIHASDLAEADRPGGPADPGLTELGERQAARL
                     VDALAPDDVAGLYVSPLARARGTAAPLAAVLGRDPEIVEDLREYDADSDHYVPVHEMA
                     RLDPAAWERMRAGLLPAYVDVPAFTARVDAAFERIIAAHPGRETAVVVAHAGVVNTWL
                     AHLLRLDRPLTFPLDYTGITRILAGRDGRRAVRTVNEIAHVGDLLTLTAGAE"
     gene            complement(5734..6159)
                     /locus_tag="VFE39_16125"
     CDS             complement(5734..6159)
                     /locus_tag="VFE39_16125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET6260164.1"
                     /translation="MSTLFSKIIDGELPGRFVWSDDRAVGFLSINPLGPGHTLVVPRE
                     EVDQWTDAEPGLLAHLTSVAHAIGAAQRSVWEPPRVGLVVVGFEVPHLHLHVFPAWEM
                     ADFDFARAAKSVDDAEQDAHADALRGALRAAGHSDNVPG"
     gene            6205..6666
                     /locus_tag="VFE39_16130"
     CDS             6205..6666
                     /locus_tag="VFE39_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HET6260165.1"
                     /translation="MARTIATNTTVDRDELTEFLRPRHHVILLTPRNDGGWQGSPVTS
                     GVDAEGRIVISTYPDRAKAGNVARHGKAAVVVLSDDFDGPWVQVDGDAELITLPDALE
                     PLVDYFRSISGEHPDWDEYRAAMTTQGKALIRITPTRWSPIATGGFPARLG"
     gene            6802..>8128
                     /gene="glgX"
                     /locus_tag="VFE39_16135"
     CDS             6802..>8128
                     /gene="glgX"
                     /locus_tag="VFE39_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HET6260166.1"
                     /translation="MRPWPGHAYPLGATYDGSGTNFAIFSEVAENVELCLFSSRGKEE
                     RIPLTEVDGFVWHGYLPNVEPGQRYGYRVHGPYDPAKALRCNPNKLLIDPYAKAVDGP
                     VEWDEAVFGYPFGDPEGRNDADSARFVPKSVVVNPFFDWGTDRSPRIPYHETVIYEAH
                     VKGLTIAHPEIPEQLRGTYTGLAHPVMIDHLKNLGVTAVELMPVHEFLDDHHLQEKGL
                     ANYWGYNTIAFLAPHHAYAMPGLRPGNQVQEFKAMVRDLHDAGIEVILDVVYNHTAEG
                     NHMGPTLSLRGIDNAAYYRLVEDDPRYYMDYTGTGNSLNVRNPHTLQLIMDSLRYWVT
                     EMHVDGFRFDLASTLAREFYDVDRLSVFFDLVQQDPVISQAKLIAEPWDVGPGGYQVG
                     NFPPLWTEWNGKYRDTVRDFWRGEPGTIGEFASRLTGSSDLYQHDGRRPY"
BASE COUNT         1340 a         2948 c         2807 g         1033 t
ORIGIN      
        1 tgctccagca ctcgtatccg cgcacgggcc cgcacgtcag ccggtggcag ccggaggatc
       61 cccagccgca gtaggcagct gggtgggggt ggggtgctgg ctcggcagcg cgagccccaa
      121 ccgcacgacg ttgcgcccgg cgcgcttggc ggcgtacagg gccgtgtcgg cgatctgcaa
      181 cagggtccgc aggtcgccac cctcggcggg ggagacggca acgccgaccg agacggtgag
      241 gcctgcgacc gtcagcgggc cgtcgggggt ggggatctcg acccgcaggt tcgcgacgcg
      301 ccgccggatc cgttcggcca ccgcttccag ctctaccgag ccgctgccct ccaggccggc
      361 gagcaggacc acgaactcct cgccgccgaa ccgccccacg aggtcacgat cgcgcacctc
      421 ggtgcgcatc gcgtcggcca cggccgcgag cacctggtca ccagcgaggt gaccatgtgt
      481 gtcgttgacc gccttgaagt ggtccaggtc gaggacgagc acgccgcgcg ggccgtcgtg
      541 ccggttcgag cgccgtagtg cgcgttcggc ctgggcgtgc caggcagcgg cgttgagcag
      601 tccggtcttg ccgtcggtgc tggccgcctc ctcgagctgc cgcacgagga cggcccggtg
      661 caggaccaac aggggcggca ggacgagcag gacgagccac gggttgatgc cgatggcgat
      721 ggccgtgatg gccccgaggc agagggtcgc tatctcgagc acgttctcgt cccatggcgc
      781 gaacaccttg gagagctcgg cctggggggt gctcaccgcg atcgcgccgg cgaccaggca
      841 gctgttgacg gtggtgtaga cgagcagggc gagcgccaac gcccacagtt gccggttgct
      901 gccggcctcg gccggtccgc ctgcacgctc gagcacggtg gcggacgcga gacacgcgag
      961 caccacggtg gcggagctga agatttgacg gtagagggga acgcgcagcc gccatgcccg
     1021 ccaccacagg tggcagtgga cgatcacggc cacgagcgcc gcgtaggagg cgggcagagc
     1081 gaccgccgcc gcgaaggtcc acaccgagct caggtcgacg tggttgccct cggtgacccg
     1141 gcgccggacg cgctcgacgc ccacggcgac ctccgtgtgc gcgatgccga gcaggcacag
     1201 gacgccggcg acctggagtt gggcgggcgt cggcggggcc tggccgagca gcccgaggac
     1261 gacgagcgcg gccgaggtgg cctcgaccag cagcacaggc gcgagaagcc gggtcgggag
     1321 cgaccagatg gcccaccgac gaacagcatt cgggtgggcc cgggaactgc ccgcctccgt
     1381 ggctccttca gatcccatcc gcgctctctg tccgctgggc gacgaaggga gcgtagtgca
     1441 gcccggcggc gatgactacc cgcacgggcg gtatcacgaa gcgctcgtgt cacagtgaag
     1501 cggctcggag cgacaggccg taacgacccc ccttccgggt cagtgaggta ggccgaatgc
     1561 acggcgcccg tgtggcccaa tcggcgcaaa ctggcgtcga tatgcaacat agtcccgcgc
     1621 tcggttcgaa cgacttgtgc gggagagcct cgcgcaacgc tcgcacaccc gtaattcgac
     1681 tgcgcaccgc aagaccggaa cctctcggag gccgcggcgt cgcgtacgaa cgaggcgagg
     1741 aggtaggcgc catgcgcaac cacaactggt gaatcgaacg accaacccca tccacaggag
     1801 ggagtgcaga cgtgcgcaac cacaactggt gaaccgacca cgtccacacc gacccggcca
     1861 aggagatgcc cgtgcgcaac cacaactggt gagcccaccc cgacccgtcg atccgctgct
     1921 tcggcccggg cctacgcccg gccacgcccg ccctgccaag gagaagcccg tgcgcaacca
     1981 caactggtga acccgctctg tcatccccgt cgagccgaag gaggaccggc atgcgcaacc
     2041 acaactggtg aaggaatttc gcgacaccca ctcgacgacc cgagttagca atgggcaacc
     2101 aattggtgat cacgatccgc taccgcgtcg cggtgggaaa agaggcagct atgcctaacc
     2161 acaactggtg agcgcacacg agcacttcgg acctcgagag gaggctctgc atgcgcaacc
     2221 acaactggtg aacacgacca ggttcccgct gcccggagga gaacccgtgc gcaaccacaa
     2281 ctggtgaccc cgcacccgcc aaccggccga tcggaggagc acccgtagtg cgcaaccaca
     2341 actggtgagc aggcccatca cccgccaccc cgggaaggag cgcgacgtgc gcaaccacaa
     2401 ctggtgaccc cgacccacgg ctcaccccac acggaggagt acgcgccatg cgcaaccaca
     2461 actggtgacg tcaggcagat cgggcaggca gatcacgtcg gcccgcgggg ctatcggcca
     2521 gtatcagccg cttgccagca cggtcgtgtc ggccggctcg cggtggtgcc ggttggcccg
     2581 gtgccacgcc acggcggctc ccagcgacag cagcgctccc accgcaccag ccaccgcgat
     2641 ggcggtggac gggtcccact cgtcggccag gaccccgccg atgagcacgg ccgcaccctg
     2701 tgccgcgatg atgccggacg ccgcgacccc gatcgctcgt ccgcgaccca ccaccggcgt
     2761 cgccctgacg aagctcgcct gggtctgcag gaggtaggcg gtggacaaca tccccgacac
     2821 cgcccacagc acgagcgtga ggggaacggc gggacggaac aggcagagca ccagcggcag
     2881 cccggccccg acagcgagca ccccgactat gcgcgcccgc gtctgcgccg ggacgaaccg
     2941 cacgaacagc catgcgccga tcacgctccc gagcgggtcg gctgccatca gtactccgac
     3001 gactgcgggg cccgcgccga gctggtccgc gtagggcgcc gcgagcccct cgggcacgac
     3061 gtaccagccg acgagccacg cgagcagcac gagcgcgcgc cgccgggggt cggcggcgat
     3121 ctcgaacagt gcggcgacgg cgccgcggag gcctccgacg ggggcgtcgg cgccggcagc
     3181 ggcgtcgccc ccttccgtgg caccgggccg tggccggtcc gccaggccgg cgcggacgag
     3241 taccgccgac aacgagaacg acgcggcgtt gcccagcagc gccatggacg ggctgatggc
     3301 ggccacgagc accccaccgc tggcgaaccc caccagctgt gcactctggc tcgtgatctg
     3361 ccggaccgcg agcccccgct cgtagagctc cccgtgcagg atctcgggca ggagcgcgcc
     3421 ctgggcggcg gtgtggggcg agccgagcag cacgacgacg acgacgagcg cacagagcgc
     3481 ccacagcgga agctgcggga tcgccatggc gccgacgagc acggcgcgca cgacgtccga
     3541 cgcgatcatg acctcacggc gtcggtaccg gtcggcgagg ccggcaagca gcaccccgcc
     3601 gagcagcgcg ggcaggaagg tgagtgcgta ggtggcggct gcccatgccg ccgagccggt
     3661 gcggccgtac accagaacgg cgagggcgac acgcgccaac tggtcgcccg cgatcgacag
     3721 cagctcggcc gcccagacga cgcggaactc ccgcacgcgg aagaccgcgc ccagtgcacc
     3781 atccgccata cggctcgttc tctccctcga cacggtgccg gggcccgcgg actcaaccgg
     3841 ctagcacgcg gcgtaagcag tcgtgttcgc ggcgtcagct tggtcgcgtc atcgtgcccc
     3901 gcgcggcggg aggttcgcaa cgcgggccgg gacaggtcgc tcccggccgg gccccggcag
     3961 atctccgagg acgtcctgca gcggcacggc tggcatcccg tgcgccgcgg ccacctccgg
     4021 ttggacgatg tggccggctg ccacgttgac gcccgccgcg agcgcgggat cgtcgccgac
     4081 ggcgccggcc aggccgcgtc ccgcgagggc caacacgtag ggcagggtgg cgttggtgag
     4141 cgcacgggtg gacgtgttcg gtaccgcgcc cggcatgttt gccacgcagt agaagatcga
     4201 ctcgtggacg ccgaacaccg ggtcgtcgtg ggtggtcggc cgggaatcgg cgaagcagcc
     4261 gccctggtcg atcgcgatgt ccaccagcac cgaacccggc cgcatccggg agacgatgtc
     4321 gttggacacc agggtcggcg ccttcgcccc cggtaccagt acggcgccga tgacgaggtc
     4381 ggcccacagg caggcgcgct ccacctcata ggcgttgctg gtgatcgtct gcaggtgacc
     4441 gcggtaggtg aagtcgatct cgcgcaaccg gttgatgttc gtgtcgagca ccatcacctc
     4501 ggcctgcatc cccaccgcga tgctggcggc gttcatcccc gagacaccgg ccccgatgac
     4561 ggcgaccttc ccggccggga ccccggacac cccgccgagc agcacacccc tcccaccggc
     4621 ggtgcgttgc aggtggtggg cgccgacctg cggggccatc cggcccgcca cctcgctcat
     4681 cggggcaagc aggggcagcg acccgtccgg cagccgcacg gtctcgtacg cgacggcggt
     4741 ggtaccggcg gtgacgagcg cgtccgtgca ctccttcgag gcggcgaggt gcaggtaggt
     4801 gaagagcacc tggtcggcgc gcagccggtg gtactcctgc ggcaccggct ccttcacctt
     4861 cagcaggagc tccgcggcac cccacacctc gtcggggtcg gcgctggtgc gggcccccgc
     4921 ggcacggaag tcgtcgtcgg gtagacctgc gcccgccccg gcgtcgcgct cgacgagcac
     4981 ctcgtggccc ctgctcgtca gctcctgcac gccggccgga gtgatcgcca cccggtactc
     5041 gtggttcttg acctcgcgcg ggactccaac cagcatccgg ggatcctgcc ttactcggcg
     5101 cctgccgtca gggtgagcag gtcgcccacg tgggcgatct cgttgacggt gcgcaccgcc
     5161 cgacggccgt cccggcccgc gaggatccgg gtgatcccgg tgtagtccag cgggaacgtc
     5221 agcgggcggt cgagccgcag aaggtgcgcg agccaggtgt tcaccacccc ggcatgcgcg
     5281 acgaccacgg cagtctcccg gccggggtgc gccgcgatga tccgctcgaa cgccgcgtcg
     5341 acccgcgcgg tgaacgccgg gacgtcgacg taagcaggta acaggccggc acgcatccgc
     5401 tcccaggcgg ccgggtcgag ccgggccatc tcgtggacgg ggacgtagtg atcggagtcg
     5461 gcgtcgtact cgcgcaggtc ctcgacgatc tcggggtcac gcccgagcac agcggcaagt
     5521 ggcgccgccg tgccgcgtgc cctggccagc gggctgacgt agagcccggc gacgtcgtcg
     5581 ggcgcgagcg cgtcgacgag ccgcgcggcc tgccgctccc ccagctccgt cagcccgggg
     5641 tcggcgggtc cgccgggccg gtcggcctcc gcgaggtcgg acgcgtggat gcgctccggc
     5701 aacgcgtgcc ggaccaggac cagctgcacc tcgtcagccg ggcacgttgt cggagtggcc
     5761 ggccgcgcgc agggcgccgc gcagggcatc ggcgtgcgcg tcctgctccg cgtcgtccac
     5821 ggacttcgcg gccctggcga agtcgaagtc ggccatctcc caggcgggga agacgtgcag
     5881 gtgcaggtgc ggcacctcga agccgacgac caccagcccc acccgcggcg gctcccagac
     5941 gctgcgctgc gccgcaccga tcgcatgcgc cacggacgtc aggtgggcga gcaggccggg
     6001 ctcggcatcg gtccactggt cgacctcctc gcgcggcacg accagcgtgt ggccgggccc
     6061 gagtgggttg atcgacagga acccgacggc acggtcgtcg gaccagacga aacgcccggg
     6121 cagctcgccg tcgatgatct tcgagaagag ggtgctcatc agatgcgacc ctagcgcccg
     6181 tgcgtggacg gctagggtcg gagcatggcc aggaccatcg ccacgaacac caccgtcgac
     6241 cgcgacgagc tcaccgagtt cctccggccg cgccaccacg tgatcctctt gacgccgcgc
     6301 aacgacggcg gttggcaggg ctcgccggtc acgtccgggg tggacgccga gggccgcatc
     6361 gtcatctcca cctaccccga ccgggcaaag gcgggcaacg tcgcgcggca cgggaaggcc
     6421 gctgtcgtgg tgctgtcgga cgacttcgac ggcccgtggg tgcaggtcga cggcgacgcc
     6481 gagctgatca ccctccccga cgcactcgaa ccgctcgtcg actacttccg ctcgatctcc
     6541 ggcgagcatc ccgactggga cgagtaccgt gccgcgatga ccacgcaggg caaggccctg
     6601 atcaggatca cacccacccg gtggagcccg atcgccaccg gcgggttccc cgcccgtctg
     6661 gggtgacgct ccgccgcatc cggcggaccg tgggggtacg ccggtaacct cttccggacg
     6721 gaccagcgct gctccgcgcc agtggtcgat cgaacgaacg gccgcggccg gacaacgact
     6781 cacgaaacac cggaggactt catgcggccg tggccaggtc acgcctaccc gctgggcgcc
     6841 acctacgacg gctcgggcac gaacttcgcg atcttctcgg aggtcgcgga gaacgtggag
     6901 ctctgcctct tcagcagccg tggcaaggag gagcggatcc cgctcaccga ggtcgacggc
     6961 ttcgtctggc acgggtacct gcccaacgtg gagcccgggc agcgctacgg ctaccgggtg
     7021 cacgggccct acgacccggc aaaggccctc cggtgcaacc cgaacaagct gctcatcgac
     7081 ccctacgcga aggccgtcga cggccccgtg gagtgggacg aggccgtgtt cggctacccg
     7141 ttcggcgacc ccgagggtcg caacgacgcc gactccgcgc ggttcgtgcc caagtccgtg
     7201 gtggtcaacc cgttcttcga ctggggaacc gaccgctcgc cacgcatccc ctaccacgag
     7261 acggtcatct acgaggcgca cgtcaagggc ctcaccatcg cgcacccgga gattcctgag
     7321 cagctgcgcg gcacgtacac ggggctcgcg cacccggtga tgatcgatca cctcaagaac
     7381 ctcggcgtga cggccgtgga gctcatgccg gtgcacgagt tcctcgacga ccaccacctg
     7441 caggagaagg ggctcgccaa ctactggggc tacaacacca tcgccttcct cgccccgcac
     7501 cacgcctacg ccatgcctgg cctgcgcccc ggcaaccagg tgcaggagtt caaggcgatg
     7561 gttcgcgacc tgcacgacgc aggcatcgag gtgatcctcg acgtggtcta caaccacacc
     7621 gccgagggca accacatggg ccccacgctg tctctgcgcg gcatcgacaa cgccgcctac
     7681 taccgcctcg tcgaggacga cccgcggtac tacatggact acaccggcac gggcaactcg
     7741 ctcaacgtgc gcaaccccca cacgctgcag ctgatcatgg actcgctgcg gtactgggtc
     7801 accgagatgc acgtcgacgg gttccgcttc gacctcgcgt ccaccctcgc ccgcgagttc
     7861 tacgacgtcg accggctgtc ggtgttcttc gacctcgtgc agcaggaccc ggtgatcagc
     7921 caggccaagc tcatcgccga gccgtgggac gtcggccccg gcggctacca ggtgggcaat
     7981 ttcccgccgc tgtggacgga gtggaacggc aagtaccgcg acaccgtgcg cgacttctgg
     8041 cggggcgagc cgggcaccat cggcgagttc gcctcccggc tcaccggctc ctcggacctg
     8101 taccagcacg acggtcgccg gccctatg
//
LOCUS       DASRSC010000332        16868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5381821,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000332 DASRSC010000000
VERSION     DASRSC010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 16868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16868
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5381821"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VFE39_16140"
     CDS             <1..444
                     /locus_tag="VFE39_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HET6260167.1"
                     /translation="DSLIEVWQANSGGRYRHLNDQWPAPLDPHFNGLGRTVTDANWHY
                     RFVTIKPGAYPWGNHHNAWRPAHIHFSLFGRSFTQRLVTQMYFPDDPLFGQDPIFNSV
                     PDPAARQRMISTFDLGATRPEWALAYRFDIVLRGREQTPFEEPHE"
     gene            437..985
                     /gene="pcaG"
                     /locus_tag="VFE39_16145"
     CDS             437..985
                     /gene="pcaG"
                     /locus_tag="VFE39_16145"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HET6260168.1"
                     /translation="MSELLTPSQTVGPFLSLGLTWPEGYLVVPPGTPGAIRIAGVLTD
                     GAGAPVGDGLIETWQADADGRFDHPDDPRGASWSGFRGFGRCATDAEGRFEIITVKPG
                     LLGDGQAPHIDVTVLARGLLDRVVTRIYFPDEAAANAADPLLGSLPPERAATLVAQEA
                     GQGELRFDIRLQGEGETVFLAV"
     gene            1138..1677
                     /locus_tag="VFE39_16150"
     CDS             1138..1677
                     /locus_tag="VFE39_16150"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET6260169.1"
                     /translation="MWGDVVEVGGAALAVTVPLDEMSRASRVRLRHPLLVQVVRYMIV
                     GGLGTVVNAVIFLVLRTWWDTMPANLVALVLSTAVSTEVNRRFTFGGIEVHRWRSYVQ
                     SGGTVLFYAFYSSTVLMLLDGVVDSPTAWEQTVVVAVASVLGGLIRFLVLRYWVFGPD
                     DHDEAGIAHTGGLRPTLRP"
     gene            1674..2672
                     /locus_tag="VFE39_16155"
     CDS             1674..2672
                     /locus_tag="VFE39_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HET6260170.1"
                     /translation="MTATQPATEAFEREPVALTERITDDGSSRWPVEPGRYRLIVARA
                     CPWANRSVIVRRLLGLEPVLSMGIAGPLHDERSWRFHLDPDNRDPVLGIEYLGEAYRK
                     ADPDFDAGITVPAVVEIASGKVVTNGFQQLTLDLSTQWTAHHRDGAPDLYPEEQRAEI
                     DEINEAVYHDVNNGVYKCGFAKGQASYERAYRGLFARLDALSERLATRRYLVGDTITE
                     ADVRLWVTLVRFDAAYHGHFKCNRQQLAEMPVLWAYARDLFQTPGFGDTIDFEQIKQH
                     YYGVHTQINPSGIVPLGPDPSGWLTPHGREELGGRPFGNGTPPGPPADDERVPPIS"
     gene            2958..3521
                     /locus_tag="VFE39_16160"
     CDS             2958..3521
                     /locus_tag="VFE39_16160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HET6260171.1"
                     /translation="MPPPPVVMLLCGSLRAGSSNDSVLRTAQAVAPAQVSAAYYRGLA
                     ELPHFNPDDDTDPLPAPVAELRAAISEAAAILICTPEYAGTLPGSFKNLLDWTVGGTE
                     ICDKPVAWVDAAAIGRGHGAEATLRAVLGYTGAHIVEAACVKIVVDPQTVGADGVITD
                     PRVREQLVGVLATLAGVQARNPELDHV"
     gene            3750..4865
                     /locus_tag="VFE39_16165"
     CDS             3750..4865
                     /locus_tag="VFE39_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HET6260172.1"
                     /translation="MCLLTVILATPGAAAASPADPAAQPSGADLGPDRLPHCAAVVAA
                     MSPRDRLAQRIMVGVDAADPATTAETVRATQVGGIFVGGNATALLTDQALRGVQAMSR
                     IPLAVAADDEGGRVQRIDDLDGELPSARKMAAQLTPEQVQQLGLQRGRQQLARGITMN
                     LAPVVDLGGQPAGAVIGDRSFGTDPDTVASYAAAFAEGERKAGVFSVLKHFPGHGRAV
                     GDSHKGRVTAPSLDELRSADLKPYATLLGPGGPLADGRTGVLVGHLDVPGLTTGLPSS
                     LTPAVYQLLRTEYGFAGLVLTDDLGAMKAVTGTFELPEAVERALAAGADMALWSSGGR
                     VSPVLDGLAQALAAGRIDPGANDAAVTRVLKAKRACS"
     gene            complement(4873..10575)
                     /locus_tag="VFE39_16170"
     CDS             complement(4873..10575)
                     /locus_tag="VFE39_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET6260173.1"
                     /translation="MTEQSFYRVVIVNRGEPAMRLIHAVREWNAEITSPRSGTDTEGR
                     TALRTIALYTAVDRHAMYVREADESVLIGPDDPEQAFAASPYLDYAELERALRVARAD
                     AVWPGWGFVSEKADFARLCRDLGIVFIGPSPEVMERLGDKIAAKRLAEEVGVPLAAWS
                     GGPVPDVEAAREHAERIGYPLMVKATAGGGGRGIRRVDAPEELEEAFERASSEASKTA
                     GDATVFLERAILGGRHVEVQVIADATGDVWTLGVRDCSVQRRNQKVIEESASTALDAE
                     QEELLRTSAAELVRAAGYVNAGTVEFLYEPKERLLSFLEVNTRLQVEHPVTEVTTGVD
                     IVKLQLHVAAGGRLADIAPEAPPARGHAMEARLTAEDPEQGFAPAPGRIEHLALPSGP
                     GIRVDTGVTTGDLIPPQFDSMIAKVIAWGRDRGEARARLSRALRQTAAVIDGGTTNKA
                     FLLDLLDRPELRSGDTDTTWLDSLMAGGYAPPRRLDVALLATAVEAQDEHVARQRERL
                     FASAERGRPEVGHETWHQVDVRAAGESYRLRVAQTRRNRYRVDVDGHVVDVDAERTGR
                     FERKLTVGGQTFAVLSVPQGSDYLVEVDGAVHRISGGEAGIVRAPAPAMVVSIPIAPG
                     DVVEAGDVVAVVESMKLETALRAPVAGRVCEVLVDPNTQVEGGTKLVRLEPDADAGTA
                     PGERSDLSGLAGPPAVGSDPGAVAADALMSLRFLVLGFDIDERDARPLLAALTAARAE
                     LPPDDPGVLAGETAVLRIFADLSALSRNRRGPEDETQQIDPAGEEARNPQEYLYAYLR
                     SRDADTEGLPESFREKLRRALAHYGVPDLEPSGTVATTALSSALYRMFLAHRRAAAHV
                     PVVLELLQWRLRHPESLPVSAREEYQRTLDRLVRATQLRHPVVGGLARRVRYTCFDAP
                     LIAAERAAGQAQVRAELDRLSDDPTVRAAQIDTIVAAAEPILGVFGERHHAAMLEVMT
                     RRYYRVRTLTDVRVEDRGGRPLLTATYSLKDGSGQHGSDQGEVEQVVLATTVHTAPDA
                     PPAGDPMAVQGDLRRLLAQLPEGRTALLDLYVIAGEAPDADPERSGEKIRAMLGRLPN
                     RLARVAVAVRRPGGDERSAWFTFRPDPADRRPVEDRTLRGLHPMVAERLGLWRLSGFE
                     LTRLPSPVDVHLFRAVGKDVPDDRRLIALSDVRELTVLRDEQGRIRALPQLEHVLDSC
                     LDALRSARAADSGDAKLDWNRVLLYVWPVVDVPLDELDSVVRVLAPRTEGLGLEQVLV
                     QFRHGGDGRDIDGRGGTANGEPREFLLRLSRPPGAGLTVQVTEPPTGPLRELDAYAQK
                     VIRARRRGAVYPYELVPMLLRDPDNGGSADTARGARGVFTEYDLDPSGAPVVVERPPG
                     GNTANIVLGTVTTPSQRYPEGMTRVVLIGDPTKALGALAEPECARVLAAIELARRFEA
                     PIEWFAVSAGAKIAMDSGTENMDWISRVLRGIIEFTQDGGEINVIVTGINVGAQPYWN
                     TEATMLMHTKGILVMTPDSAMVLTGKQSLDYSGGVSAEDNFGIGGYDRIMGPNGQAQY
                     WAPDLSGAVDVLLAHYAHTYRAPGERFPRPAHTADPVDRDISDSPHSGPGCDFATVGE
                     VFTANPGRKKPFDIRSVLHAVADADHPTLERWVDMIDAECVVVLDAHLGGQPVALLGI
                     ESRPLPRRGRPPVDGPTAFTAGTLFPKSSKKTARAINAASGNRPLVILANLSGFDGSP
                     ESLRQLQLEYGAEIGRAVVNFDGPIVFCVVSRYHGGAFVVFSATLNDNMEIAAVEGSY
                     ASVLGGAPAAAVVFAGEVNKRTAADPRIADLETRIAEAQQAGSESEAARLTAELAALR
                     PGVRSEKLGQVADEFDAAHSVERAQRVGSVHEIIPATRLRPYLAEAVQRGMTKTGPRR
                     "
     gene            10745..11599
                     /locus_tag="VFE39_16175"
     CDS             10745..11599
                     /locus_tag="VFE39_16175"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6260174.1"
                     /translation="MLTVTRGDLTFDVHEHGSGDDVPALLLHGFPQRGTAWDGVAERL
                     AAHGYRTLAPDQRGYSPCARPRGRSAYRLTDLVGDALAVVDALVGAGGRVHVVGHDWG
                     AAVAWRLAARHPDRVRSLTAVSVPPPATYVRSLFTTRQALASWYVYALQLPALPERVL
                     RAEGRPFSAALVAGLRRTGQGRTAAERDAAGLADPSALTAAINWYRGGLRGTRGDVDP
                     PVQVPTLFVWSDGDTAITRQATELTQQQVAGPFRYVELRGVSHWILDESPGQLADLLL
                     DQMGEYPG"
     gene            11647..12015
                     /locus_tag="VFE39_16180"
     CDS             11647..12015
                     /locus_tag="VFE39_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6260175.1"
                     /translation="MEVLASRVLLHPVDMQRSVAFYRDVLGLAVAREFPGGTVFFLGG
                     GFLEVSGTADGPPGDAVALWLQVRDLAAAHRELAEHVVREPRQEPWGLREMWISDPNG
                     TRIVLVQVPEDHPIRRDTRH"
     gene            complement(12036..13472)
                     /locus_tag="VFE39_16185"
     CDS             complement(12036..13472)
                     /locus_tag="VFE39_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6260176.1"
                     /translation="MGVVAPSARPTLVIGVLASCGLAVSLMQTLVVPLLPQFPRLLSA
                     SPGTVAWLVTATLVAGAVCAPVLGRLGDMYGKRRILLVALAMVTIGSALGAVAPGVGL
                     LIAARVLQGASLGVIALGISIMRDLLPPDRVGSGIALMSSSLGIGGAICLPITGLVAQ
                     HASWRWLFAGAAVLGALQFLLVRKVVRESPQRSGGRFDLPGAVGLALALVCVLLAISK
                     GTEWGWGSARVIGLLAAALVLFALWSRLELRARSPLVDLRVSARPEVLWTNIASILIG
                     FGMFAGFLVTTQVLQAPVATGYGFGLSLLAAGVVLLPIGGAMTIFSPVSARVSRRRGA
                     RTTLVLGTVVLAVGNVAMATLPASIPLVMAASTVTAIGAALAYSALPLLIMDAVPTSE
                     TAAANSLNTLMRMLGTSSCSAVVAAVTTGMTTVVGGRLLPSGDAYTVVFLAAAAAALG
                     GAAIAAFTPRAKAAGPSTPAPVPAPARA"
     gene            13711..15042
                     /locus_tag="VFE39_16190"
     CDS             13711..15042
                     /locus_tag="VFE39_16190"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HET6260177.1"
                     /translation="MTRAQSAPDFAIASLGPCRITSPMAPLLESRRTTEHYVDEHDRV
                     LLDDTLAGITSRGVPPGELPGMEPCGPRSKIFFDPAKTRAAIVTCGGLCPGLNDVIAG
                     LVRTLTYHYRVRRVVGIRNGYQGFVADYGHDVIELTPESVRDIATDGGTVLGTSRGPQ
                     DPEEIVDCLERLHVNVLFVVGGDGSMRGAMQIAHTVAERGLRLAVVGIPKTIDNDIPY
                     IDQSFGFQTAFSLASDAIRAAQVEAKSTANGIGLVKLMGRHSGFIACYAALARSGADV
                     VLIPEVPFGLDGDQGLLTHLRRRVAERGHAVVVAAEGAGQELLGGSGDASDASGNARL
                     QDIGPYLRRRIGDHFADAGVETSIRYIDPSYAIRSVPANPYDSVYCVRLSQAAVHAAM
                     SGRTEMVVGRYRRRFVHIPMKVAVSRRNQVDPHGDLWMAVLESTGQPVRFA"
     gene            15083..15889
                     /locus_tag="VFE39_16195"
     CDS             15083..15889
                     /locus_tag="VFE39_16195"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009077365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6260178.1"
                     /translation="MTAPEDFPRTAIVTGSDSGIGRATAVALAAAGCDVGITWHSDEA
                     GAQDTADEVRAHGRKAEIRQLDLTALPGAAAVVDDLADALGGVDVLVNNAGTGTRTLL
                     LDIDYETWRSVLAVDLDGAFLCLQRAARRMIDVGRGGRIVNITSVHEHQPRVGAGPYC
                     AAKGGLGLLTKVAALELAEHGITVNAVAPGEIATPMTGQEDEDPRLVVRPGVPLRRPG
                     DAREVAAVVAFLASSAASYVTGASWPVDGGMLQMGPMAGSHLTSDDWRRP"
     gene            15933..16754
                     /locus_tag="VFE39_16200"
     CDS             15933..16754
                     /locus_tag="VFE39_16200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6260179.1"
                     /translation="MHGLAMRFLGHSTVRMELAGRVVLTDPVLTSGIGPLRRVGPLPA
                     PGDHADVDLVLISHLHGDHLHLPSLRRLPRSARVVVPRGAGGWLRARGIARVEELAPG
                     EHLDDGDLRVTGVPATHSGHRWGPRSTRGPQAQAMGHLLASPDVRVYAAGDTDLFPGM
                     AALAGQPLDVALLPVWGWGPTLGAGHLDPARAAAAVELLRPRVAVPVHWGTLALAGLA
                     RVPGRPGARMRRLLVEPPREFAAAVVAAGGTTRVAVTEPGAAVVLPAPAADSEMS"
     gene            16760..>16868
                     /locus_tag="VFE39_16205"
     CDS             16760..>16868
                     /locus_tag="VFE39_16205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET6260180.1"
                     /translation="MSGSWTDAASIGYPALFGGVLLGSLVPVVPTGAVVG"
BASE COUNT         2301 a         6126 c         6115 g         2326 t
ORIGIN      
        1 gattcgctca tcgaggtctg gcaggccaat agcggcggca ggtaccgcca cctgaacgac
       61 cagtggccgg ccccgctcga tccgcacttc aacggtctcg gccgcaccgt cacggacgcg
      121 aactggcact accggttcgt caccatcaag cccggcgcct acccgtgggg caaccaccac
      181 aacgcctggc gccctgcgca catccacttc tcgttgttcg gccgctcgtt cacgcagcgg
      241 ctcgtgaccc agatgtactt cccggacgac ccgctgttcg gccaggaccc gatcttcaac
      301 tccgtgcccg atcccgctgc ccggcagcgg atgatctcga cattcgacct gggcgccacc
      361 cgcccggagt gggcgctggc gtaccgcttc gacatcgtgc tccgcggccg cgagcagacc
      421 ccgttcgagg agccgcatga gtgagctgct gaccccgtca cagaccgtcg ggccgttcct
      481 gagcctcggg ctgacctggc ccgaggggta cctcgtcgtc ccgcccggaa caccgggggc
      541 gatccggatc gccggcgtgc tcaccgacgg tgcgggcgca cccgtcggcg acggcctgat
      601 cgagacctgg caggccgacg ccgacggccg gttcgaccac ccggacgacc cgcggggggc
      661 ctcctggtcg gggttccggg gcttcgggcg ctgcgccacc gacgccgagg gtcgcttcga
      721 gatcatcacg gtcaagcccg gtctgctcgg tgacgggcag gccccgcaca tcgatgtcac
      781 ggtgctggcc cgcggactgc tcgaccgtgt cgtcacgagg atctacttcc ccgacgaggc
      841 cgccgccaac gcggccgacc cgctgctcgg ctcgcttccg cccgagcgcg ccgccacgct
      901 ggtggcgcag gaggcgggcc agggcgagct gcgcttcgac atccgcctcc agggcgaggg
      961 cgagacggtc ttcctggccg tctgactgcc gtcttgcccc gctccgtccc cgtgttcccg
     1021 atcacttcac gatgtggcga cgaaccggaa tcgatgatcg ctcacagcgt tgtcacaggt
     1081 gagggacagc gcgaacttcg gttcccctgc gataaaggga tcacgaggtt cgcggagttg
     1141 tggggggacg tggtcgaggt ggggggcgca gcgcttgcgg tgaccgtgcc gctggacgag
     1201 atgtcgaggg cgtccagggt gcggctgcgg cacccgttgc tggtgcaggt ggtccggtac
     1261 atgatcgtcg gtggcctcgg caccgtcgtg aacgccgtga tcttcctggt gctgcggacg
     1321 tggtgggaca ccatgccggc caacctcgtc gccctcgtgc tgagcacagc ggtgagcacc
     1381 gaggtgaacc ggcggttcac cttcggcggc atcgaggtcc accggtggcg gtcctacgtg
     1441 cagagcggcg gcacggtgct gttctacgcg ttctacagct ccacggtgct gatgctcctc
     1501 gacggggttg tcgactcgcc gacggcgtgg gagcagacgg tggtggtggc cgtggcgagc
     1561 gtgctcggcg gactgatccg gttcctcgtg ctgcggtact gggtcttcgg cccggacgac
     1621 cacgacgagg cggggatcgc gcacaccggc ggtctgcggc ctacattgcg accatgactg
     1681 ccacgcagcc cgccacggag gcgttcgagc gcgaaccggt ggcgctcacc gagcggatca
     1741 ctgacgacgg gtcttcgcgc tggccggtgg aaccggggcg ctaccggctg atcgttgcca
     1801 gggcatgccc gtgggcaaac cgcagcgtca tcgtccgcag gctgctcggg ctggagccgg
     1861 tgctgtcgat gggcatcgcc ggacccctgc acgacgagcg gagttggcgg ttccacctcg
     1921 accccgacaa ccgggacccg gtgctcggca tcgagtacct cggcgaggcc taccgcaagg
     1981 ccgaccccga cttcgacgcc ggcatcaccg tgcccgcggt cgtggagatc gccagcggga
     2041 aggtggtgac caacgggttc cagcagctca ccctcgacct ctccacgcag tggacggctc
     2101 accaccgcga cggcgcgccc gacctctacc cggaggagca gcgcgcggag atcgacgaga
     2161 tcaacgaggc cgtctaccac gacgtcaaca acggcgtcta caagtgcggc ttcgcgaagg
     2221 ggcaggcgtc gtacgagcgt gcctaccggg ggctgttcgc ccggctcgat gcgctgtcgg
     2281 agcggcttgc cacccggcgc tacctcgtgg gcgacacgat caccgaggcc gacgtgcggc
     2341 tgtgggtcac actggtgcgc ttcgacgccg cctaccacgg ccatttcaag tgcaaccgcc
     2401 agcagctggc cgagatgccg gtgctgtggg cgtacgcgcg cgacctgttc cagacgcccg
     2461 gcttcggtga cacgatcgac ttcgagcaga tcaagcagca ctactacggc gtgcacaccc
     2521 agatcaaccc gtcaggaatc gtgccgctcg ggcccgaccc gagcggctgg ctcaccccgc
     2581 acgggcggga ggagctcggc ggccgcccgt tcgggaacgg caccccgccc gggccgccag
     2641 cggacgacga gcgcgtgccg ccgatcagct gagcagctcg tagccggcgc ctgccacctc
     2701 gtcggtcgcg gtggcgcgcc ggtcgaggct cagcagcacg gcgccggcct ggccggcgct
     2761 gagcgcgacc acgaggtcgt acaccgcgcc cccgacgatg cccgagcgtc gtgccgccgc
     2821 gaggaggtcg gagggcgggg cgccctgcag tcgcggggtc gacgggggaa gtggccgctg
     2881 aggaaggccg tgacgagagg cgcaggcacc cgctgctgcg gcggcgggac gggatatcgg
     2941 ctcggtaggt tgggtaaatg cctccaccac cggtagttat gttgctgtgc ggaagcctgc
     3001 gggccggttc ctccaacgac tccgtgctgc gcaccgcaca ggccgtcgct cccgcacagg
     3061 tgagtgccgc ctactacagg gggctggcgg agcttccgca tttcaatccc gacgacgaca
     3121 ccgatcccct gccggctccc gtggccgagc tgcgtgcggc gatctcggaa gcggccgcga
     3181 tcctgatctg caccccggag tacgcgggca ccctgcctgg ttcgttcaag aacctgctgg
     3241 actggaccgt cggcggcacc gagatctgcg acaagccggt ggcctgggtc gacgcggccg
     3301 ccatcggccg tggtcacggt gccgaggcca cgctgcgggc ggtactcggt tacaccggtg
     3361 cccacatcgt ggaggcggcc tgcgtgaaga tcgtcgtgga tcctcagacg gtcggcgcgg
     3421 acggcgtcat caccgacccg cgggtgcgcg agcagctcgt cggggtgctg gcgacgctgg
     3481 ccggggtcca ggcacgcaac ccggagctcg accacgtcta gtcgacgacg aggcattcgc
     3541 gtgggagggc gaccgggatg ccggccttcg cgcgctggac acccagctgg tggcctgagt
     3601 ccgcgatccc gcgcgtgacc cgggcgaccg ccgggggaat gatggccgcg atgccgaccc
     3661 gtgcgccgtc tccgtcgtcc ccgcagtcgc cagcgccgtt gccgctgagt cacggccggg
     3721 gccggacacg tgccggtgcc gccctgctgg tgtgtctcct cacggttatc ctcgcgacac
     3781 ccggtgccgc cgcggcatcg cccgccgacc cggcggccca gcccagcggt gcggacctcg
     3841 gccccgaccg gctgccccac tgcgctgccg tggtcgccgc gatgtcaccg cgcgaccggc
     3901 tcgcgcagcg gatcatggtg ggggtcgacg ccgccgaccc ggccaccacc gccgagacgg
     3961 tccgcgccac gcaggtgggt gggatcttcg tgggcggcaa cgccaccgcg ctgctcaccg
     4021 accaggccct gcggggcgtg caggcgatgt cccgcatccc gctcgccgtc gccgcggacg
     4081 acgagggcgg ccgggtgcag cgcatcgacg accttgacgg cgagctgccc agcgcccgga
     4141 agatggcagc gcagctcacc ccggagcagg tgcagcaact cggcctccag cggggccggc
     4201 agcagctcgc ccgcggtatc acgatgaacc tagccccagt ggtcgacctc ggcggccagc
     4261 cggcgggcgc tgtgatcggg gaccggtcct ttggcacgga ccccgacaca gtggcgagct
     4321 acgcggccgc gttcgcggag ggcgagcgga aggcgggcgt gttcagcgtg ctcaagcact
     4381 tccccggtca cggccgcgcg gtcggcgact cgcacaaggg ccgggtcacc gccccgtcgc
     4441 tcgacgagct gcgcagcgcc gacctgaagc cctacgccac cctgctgggc ccgggcggcc
     4501 cactcgccga cgggcgcacc ggcgtgctcg tcgggcacct ggatgtgccg gggctcacca
     4561 ccggcctgcc cagctcgctc acacccgcgg tctaccagct gctgcgcacc gagtacggct
     4621 tcgccgggct cgtgctcacc gacgacctgg gcgccatgaa ggccgtgacc ggcacgttcg
     4681 agctccccga ggccgtcgaa cgggcgctgg ccgccggtgc cgacatggcg ctgtggtcca
     4741 gcggcggccg cgtcagcccg gtgctggacg ggctggcgca ggcgctggcc gcaggccgca
     4801 tcgaccccgg ggcgaacgac gccgcggtga cgcgcgtcct gaaggccaag cgcgcctgct
     4861 cctgatcccg gcctaccggc gggggcccgt cttcgtcatt ccgcgttgca cggcctccgc
     4921 cagatagggg cgcaggcgcg tcgccgggat gatctcgtgc accgagccga cccgctgcgc
     4981 ccgctctacg ctgtgcgccg cgtcgaactc gtcggccacc tgcccgagct tctcggagcg
     5041 gacgccgggg cgcagtgccg cgagctcggc ggtgaggcgc gccgcctcgg actccgagcc
     5101 ggcctgctgg gcctcggcga tccgcgtctc gaggtcagcg atgcggggat cggcggcggt
     5161 gcgtttgttc acctcgcccg cgaacacgac ggccgccgca ggcgccccgc cgagcaccga
     5221 cgcgtaggag ccctcgacgg cggcgatctc catgttgtcg ttgagcgtcg cggagaacac
     5281 gacgaacgcg ccaccgtggt agcgggagac cacgcagaat acgatcgggc cgtcgaagtt
     5341 gaccaccgcc cggccgatct cggcgccgta ctcgagttgg agctggcgca acgactccgg
     5401 ggagccgtcg aagcccgaga ggttcgccag gatcaccagt ggccggttgc cgctggccgc
     5461 gttgatcgcc ctggcggtct tcttcgacga cttcgggaac agcgttcccg ccgtgaacgc
     5521 cgtcggcccg tccaccggcg gccgcccgcg gcgggggagc ggacgcgact cgatgccgag
     5581 cagcgcgacc ggctgcccgc ccaggtgcgc gtcgagcacc accacgcact cggcgtcgat
     5641 catgtcgacc cagcgctcca gcgtggggtg gtcggcgtcg gccaccgcgt gcagcaccga
     5701 gcggatgtcg aacggcttct tgcgccccgg gttcgccgtg aacacctcgc ccaccgtcgc
     5761 gaagtcgcag cccgggccgg agtgcgggga gtcgctgatg tcgcggtcca ccggatcggc
     5821 ggtatgggcc gggcggggga accgctcgcc gggcgcccgg taggtatgcg cgtagtgcgc
     5881 gagcagcaca tccaccgcac cggacaggtc gggagcccag tactgcgctt gcccgttcgg
     5941 gcccatgatc cggtcgtagc cgccgatgcc gaagttgtcc tctgcgctca cgccgccgga
     6001 gtagtcgagc gactgcttgc cggtgagcac catcgcgctg tcgggcgtca tcacgaggat
     6061 gcccttggtg tgcatgagca ttgtcgcctc ggtgttccag tacggctggg cgccgacgtt
     6121 gatgccggtg acgatcacgt tgatctcgcc gccgtcctgg gtgaactcga tgatcccgcg
     6181 cagcacccgg gagatccagt ccatgttctc ggtaccggag tccatcgcga tcttggcccc
     6241 cgccgacacc gcgaaccact cgatcggcgc ctcgaaacgg cgggcgagct cgatcgcggc
     6301 gagcacgcgc gcgcactccg gttcggcgag cgcgccgagg gccttggtcg ggtcgccgat
     6361 caggacgacg cgcgtcatgc cctccgggta gcgctgcgac ggagtcgtca cggtgccgag
     6421 cacgatgttg gcggtgttgc cgcccggcgg acgttcgacc acgaccgggg cgccgctcgg
     6481 atcgaggtcg tactcggtga acacgccccg ggcaccgcgc gcggtgtcgg cggagccacc
     6541 gttgtcgggg tcgcgcagca gcatcgggac cagctcgtac gggtagaccg ccccgcgacg
     6601 gcgcgcccgg atcaccttct gcgcgtaggc gtccagctcg cgcagcgggc cggtcggcgg
     6661 ctcggtgacc tggacggtga gcccggctcc cggcgggcgg gacaaccgca gcaggaactc
     6721 acgcggctcg ccgttcgcgg tgcccccgcg tccgtcgatg tcccgtccgt cgccgccgtg
     6781 gcggaactgc accagtacct gctccaggcc gagcccctcg gtgcgcggcg cgaggacgcg
     6841 gaccaccgag tccagctcgt cgagcggcac gtcgaccacc ggccagacgt agagcaggac
     6901 ccggttccag tccagcttgg cgtcgccgct gtcggcggcg cgtgctgaac gcagggcatc
     6961 gaggcacgag tcgaggacgt gctccagctg cggcagcgcc ctgattcggc cctgctcgtc
     7021 gcgcagcacc gtcagctcgc gcacgtcgga cagcgcgatc agcctccggt cgtcgggcac
     7081 gtccttgccc accgcgcgga acaggtgcac gtcgaccggc gacggcagcc gggtcagctc
     7141 gaacccggac agccgccaca gcccgagccg ctcagccacc atcgggtgca gcccgcgcag
     7201 cgtgcggtcc tcgaccgggc ggcgatcagc cgggtcgggc cggaacgtga accaggccga
     7261 ccgctcatcg ccaccgggtc tacgcaccgc cacggcgacg cgtgcgagcc ggttgggcag
     7321 cctgccgagc atcgcgcgga tcttctcccc ggagcgctcc gggtcggcgt ccggtgcctc
     7381 accggcgatg acgtagaggt cgagcaatgc ggtgcggccc tccggcagct gcgcgagcag
     7441 ccggcgcagg tcgccctgca ccgccatcgg gtcgcccgcc ggcggcgcgt ccggcgcggt
     7501 gtgcaccgtg gtggccagca cgacctgctc gacctcgccc tggtcggagc cgtgctggcc
     7561 ggacccgtcc ttgagcgagt aggtcgcggt gagcagcggg cgcccgcccc ggtcctcgac
     7621 ccggacgtcg gtcaacgtcc gcacgcggta atagcgcctt gtcatcacct cgagcatcgc
     7681 cgcgtggtgc cgctcgccga acacaccgag gatcggctcg gccgccgcca cgatcgtgtc
     7741 gatctgcgcc gcgcggacgg tggggtcgtc ggacagccgg tccagctccg cgcgcacctg
     7801 ggcctggccc gctgcccgct cggcggcgat cagcggggcg tcgaagcagg tgtagcgcac
     7861 gcggcgggcc agtccaccga cgacggggtg ccgcagctgc gtggcccgga cgaggcggtc
     7921 gagcgtgcgc tggtactcct cgcgggccga caccggcaac gactcggggt ggcgcagccg
     7981 ccactgcagc agctcgagca ccaccggaac gtgcgcagcg gcccggcggt gggccaggaa
     8041 catccggtag agcgcgctgc tcagcgcggt cgtcgcgacc gtgccggacg gctccaggtc
     8101 cggcacgccg tagtgcgcga gcgcacggcg cagcttctcc cggaacgact ccggcaaccc
     8161 ttcggtgtcg gcgtcgcggg agcgcaggta ggcgtacagg tactcctgcg ggttgcgggc
     8221 ctcctcgccg gccgggtcga tctgctgcgt ctcgtcctcg gggccgcggc ggttgcgtga
     8281 cagcgcgctc aggtcggcga aaatgcgcag gacggccgtc tccccggcga gcacaccggg
     8341 gtcgtccgga gggagctcgg cacgggccgc ggtgagcgcg gccagcagcg ggcgggcgtc
     8401 gcgctcatcg atgtcgaatc cgagcaccag gaaccgcagc gacatcagcg cgtcggccgc
     8461 cacagccccg gggtcagacc ccaccgccgg tggccccgcc agcccggaca gatcggaccg
     8521 ctcgcccggg gcggtacctg cgtcggcgtc gggctccagg cggacgagct tcgtcccgcc
     8581 ctccacctgg gtgttggggt cgacgagcac ctcgcacacc cgccctgcga caggcgcgcg
     8641 aagcgccgtc tccagcttca tgctctcgac gacggccacc acgtcgcctg cctcgacgac
     8701 atcgcccggc gcgatcggga tcgagaccac catcgccggc gccggggcgc ggacgatccc
     8761 ggcctcgcca ccggagatgc ggtgcaccgc gccgtcgacc tcgacgaggt agtccgagcc
     8821 ctgaggcacc gagagcaccg cgaaggtctg cccgccgacg gtgagcttgc gctcgaagcg
     8881 ccccgtgcgc tccgcgtcga cgtcgacgac gtgaccgtcc acgtccaccc ggtaccggtt
     8941 gcgccgggtc tgggccaccc gcagccggta ggactcgccc gccgcgcgta cgtccacctg
     9001 gtgccatgtc tcgtgcccca cctcggggcg cccacgctcg gccgaggcga acagccgctc
     9061 gcgctgccgc gcaacgtgct cgtcctgcgc ctcgacggcg gtggcgagca gcgccacgtc
     9121 gagccgccgg ggcggggcgt agccaccggc catcaggctg tcgagccagg tggtgtcggt
     9181 gtcgccggag cggagctccg ggcgatccag caggtcgagc aggaacgcct tgttggtggt
     9241 gccgccgtcg atcaccgcgg cggtctggcg cagcgcccgg gagagccgcg cacgcgcctc
     9301 gccgcggtcc cggccccacg cgatcacctt ggcgatcatc gagtcgaact ggggggggat
     9361 caggtcgccc gtggtgacgc cggtgtccac ccggatgccc ggtccgctgg gcagggccag
     9421 gtgttcgatg cgaccgggtg ccggtgcgaa gccctgctcg gggtcctcgg ccgtcagccg
     9481 cgcctccatg gcgtgcccgc gcgcgggtgg ggcctccggt gcgatgtccg cgagccggcc
     9541 gcccgcggcg acgtgtagct gcagcttgac gatgtcgacg ccggtggtga cctcggtgac
     9601 cggatgctcc acctgtagcc gcgtgttgac ctccaggaac gacagcagcc gttccttggg
     9661 ctcatacagg aactcgaccg tgccggcgtt gacgtagccc gccgcccgca ccagctcggc
     9721 cgcggacgtc cgcagcagct cctcctgctc ggcgtcgagc gcggtggacg ccgactcctc
     9781 gatcaccttc tggttgcgcc gctgtaccga gcagtcgcgg accccgagcg tccagacatc
     9841 gccggtggcg tcggcgatca cctgcacctc gacgtggcgc ccgccgagga tcgcccgctc
     9901 gaggaagacg gtggcatcgc ccgcggtctt cgacgcctcc gagctcgctc gctcgaaggc
     9961 ctcctccagc tcctccggcg cgtcgacccg ccggataccg cgcccaccgc cccccgcggt
    10021 ggccttgacc atcaacgggt agccgatccg ttccgcgtgc tcccgggcgg cctcgacgtc
    10081 ggggaccggg ccgccgctcc acgcggccag cggcacgccg acctcctcgg cgagccgctt
    10141 cgccgcgatc ttgtcgccga gccgctccat cacctcgggc gacggcccga tgaacacgat
    10201 cccgaggtcc cggcacagcc gggcgaagtc ggccttctcg gagacgaagc cccagcccgg
    10261 ccacacagca tcggcccgcg ccacgcgcaa tgcgcgctcg agttccgcgt aatcgaggta
    10321 ggggctcgca gcgaaggcct gctcggggtc gtccggcccg atcaggacgg actcgtcggc
    10381 ctcgcggacg tacatcgcgt gccggtccac cgccgtgtac agcgcgatcg tgcgcaaggc
    10441 cgtgcggccc tcggtgtcgg tgccggatcg tggcgaggtg atctcagcgt tccactcccg
    10501 caccgcgtgg atgagccgca tcgccggctc gccccggttg acgatgacga cccggtagaa
    10561 cgactgctcc gtcacgatct actcactctc gtcgctggtg ccaggggccg gccccgcccg
    10621 tggcgcttcc ggcggaccgg agcgcccgga actcctggcc cgggcccacc caactcgtcc
    10681 tcccgccatc gcacaactga cgagcgcgta accgcccacc gaggggcgca tgcatgatga
    10741 tcccgtgctg acggtgacgc ggggcgacct gaccttcgac gtgcacgagc atggctccgg
    10801 cgacgacgtc ccggcgctgc tgctgcacgg cttcccgcag cgcggcaccg cctgggacgg
    10861 ggtcgcggaa cggctcgccg cgcacggcta ccggaccctc gctcccgacc agcgcggcta
    10921 ctcgccgtgc gcgcgtccgc gtggccggag tgcctaccgg ctcaccgatc tcgtcgggga
    10981 cgcgctggcg gtggtggatg ccttggtcgg tgcgggcggg cgcgtgcacg tggtcgggca
    11041 cgactggggt gccgcggtcg cctggcgcct ggcggcgcgg caccccgacc gcgtccgctc
    11101 gctcaccgcg gtgtccgtcc ccccgccggc gacgtacgtg cgctcgctgt tcaccacccg
    11161 gcaggcgctc gcgtcctggt acgtctacgc gttgcagctc cctgcgctgc cggagcgggt
    11221 gctccgggcc gaaggccgcc cgttctcggc ggcactggtc gcgggtctgc ggcggaccgg
    11281 gcagggaagg acggcagcgg agcgggacgc ggccgggctc gccgacccgt cggcgctgac
    11341 cgcggcgatc aactggtacc gcggagggct ccgcgggacg cgcggtgatg tggacccacc
    11401 cgtacaggtg cccacgctgt tcgtctggag cgacggcgac accgccatca cccggcaggc
    11461 aacggagctg acgcagcagc aggtcgccgg tcccttccgc tacgtggagc tgcgcggcgt
    11521 gagccactgg atcctcgacg agtcgcccgg tcagctcgcc gatctgctcc tcgaccagat
    11581 gggcgagtac ccgggctgac gtcgcgacac tagggtcccg gccatggcca gcgtccggtc
    11641 gtcctgatgg aggtcctcgc ctcccgggta ctgctgcacc cggtcgacat gcagcgcagc
    11701 gtcgcgttct accgggacgt gctcgggctc gcggtggccc gggagttccc gggtggcacg
    11761 gtgttcttcc tgggcggtgg cttcctggag gtctccggga ccgccgacgg gccgccgggg
    11821 gacgcggtcg cgctctggct gcaggtgcgt gacctcgccg ccgctcaccg tgagctcgcc
    11881 gagcatgtcg tgcgggagcc ccgccaggag ccgtggggcc tgcgcgagat gtggatctcg
    11941 gatccgaacg gtacgaggat cgtgctggtc caggtgccgg aggaccatcc gattcgccgg
    12001 gacacgcgcc actaggagcg cgtggaatcg ccccgtcatg cccgtgccgg cgcgggcacc
    12061 ggcgcgggag tgctcggccc ggccgccttc gcccgcgggg tgaaggccgc gatcgccgcc
    12121 ccacccagtg ccgcggccgc cgccgcgagg aacacgacgg tgtacgcgtc gccggagggc
    12181 agcagccgtc cgccgaccac cgtggtcatg ccggtggtga ccgccgccac caccgcgctg
    12241 caggaggagg tgccgagcat ccgcatcagc gtgttgaggc tgttcgccgc tgctgtctcg
    12301 gacgtgggca ccgcgtccat gatgagcagc ggcagcgcgg agtaggcgag cgcggcgccg
    12361 atggcggtga cggtggatgc ggccatcacc agcgggatcg acgcgggcag cgtcgccatc
    12421 gcgacgttgc cgaccgcgag cacgacggtg ccgagtacga gcgtggtgcg ggcgccgcgg
    12481 cgacgcgaca cgcgggcgga gaccggcgag aagatcgtca tcgcccctcc gatcgggagc
    12541 agtacgaccc cagcggcgag gagcgacagc ccgaacccgt acccggtcgc gacgggcgcc
    12601 tgcaggacct gcgtggtcac gaggaacccg gcgaacatgc cgaacccgat gaggatcgac
    12661 gcgatgttgg tccagagcac ctcgggccgt gccgagaccc gcaggtccac cagcggcgag
    12721 cgcgcccgca gctccaacct gctccagagc gcgaacagca ccagagcggc ggcgagcagg
    12781 ccgatcacgc gggcgctgcc ccagccccac tcggtgccct tggagatcgc gaggagcacg
    12841 cagaccagcg cgagggcgag cccgacggca cccggcaggt cgaagcggcc gccggagcgc
    12901 tgcggcgact cccgcaccac cttgcggacc aggaggaact gcagggcgcc gagcacggcg
    12961 gctcccgcga agagccagcg ccagctcgcg tgctgcgcga cgaggccggt gatcggaagg
    13021 cagatcgcgc cgccgatgcc gagcgaggag ctcatcaacg cgatgccgct cccgacccgg
    13081 tcgggcggca gcaggtcgcg catgatgctg atgccgagcg cgatcacgcc cagcgacgcg
    13141 ccctgcagca cgcgggccgc gatcaggagc ccgacgccgg gcgcgacggc gcccagcgcc
    13201 gacccgatgg tcaccatcgc gagcgccacg agcaggatgc ggcgcttgcc gtacatgtcg
    13261 ccgagcctgc ccagcacggg tgcacacacc gcgcccgcca ccagcgtggc cgtgacgagc
    13321 cacgccaccg tcccgggcga cgccgagagc aggcgcggga actgtggcag gagcggaacg
    13381 accagggtct gcatcagcga gacggcgagg ccgcacgagg caaggacgcc gatgacaaga
    13441 gtcggacgag cagacggtgc gaccacgccc atgaggtgac cacgtaattg ttgtcattag
    13501 caagcgtttt gtcgccttcg ataggttcgt cacgtgccgg ggggcgcgac ttgcggcccg
    13561 gcctagccag cgcatggccg gcagggcatc ctggaacggt caagaaggcc atggtcccgg
    13621 cgctgcggcg tggtcgtccg tcccgtcgat gcagggagcg tcgccgtgag cacagccggc
    13681 tcggacgatc gacaggggag cgtcgtcgag ctgacccgcg cccaatccgc gccggacttc
    13741 gccatcgcga gcctcggacc gtgccgcatc acctcgccga tggctccgct gctcgagtcc
    13801 cggcgcacga cagagcacta cgtcgacgag cacgaccgcg tgctgctcga cgacaccctc
    13861 gccggcatca cctcgcgtgg ggttcccccg ggagagctgc cggggatgga gccgtgcggg
    13921 ccccgtagca agatcttctt cgacccggcc aagacccgcg ccgcgatcgt cacctgcggc
    13981 ggcctgtgcc cgggtctcaa cgatgtgatc gccgggctcg tccggacgct gacctaccac
    14041 taccgggtgc gcagggtggt cggcatccgc aacggctacc aggggttcgt cgccgactac
    14101 gggcacgacg tcatcgagct cacgccggag tcggtgcggg acatcgcgac cgacggcggc
    14161 accgtgctcg gcacgtcacg tgggccgcag gaccctgagg agatcgtcga ctgcctcgag
    14221 cgcctgcacg tcaacgtgct gttcgtcgtc ggcggcgacg gttcgatgcg cggggcgatg
    14281 cagatcgccc acacggtggc ggagcgcggg ctccggctcg ccgtcgtcgg catcccgaag
    14341 accatcgaca acgacattcc ctacatcgac cagagcttcg gcttccagac cgccttcagc
    14401 ctcgccagcg acgcgatcag ggctgctcag gtcgaggcga agtcgacggc gaacggcatc
    14461 gggctggtca agctcatggg ccgacactcc ggcttcatcg cgtgctacgc cgcgctggcc
    14521 cgcagcgggg ccgacgtcgt gctgatcccg gaggtgccct tcgggctcga cggcgaccag
    14581 gggctgctca cgcacctgcg ccggagggtc gccgagcgag ggcacgccgt cgtcgtcgcg
    14641 gccgaagggg cgggtcagga gctgctgggc gggtcaggtg acgcgagcga cgcctccggc
    14701 aacgcccgcc tgcaggacat cggcccgtac ctgcggaggc ggatcggcga ccacttcgcc
    14761 gacgccggcg tggagacctc gatccgctac atcgatccca gctacgccat ccgcagcgtg
    14821 cccgccaacc cgtacgactc ggtgtactgc gtgcgcctgt cgcaggcggc ggtgcacgcg
    14881 gcgatgtcgg gacgcacgga gatggtggtg ggccggtacc ggcggcggtt cgtgcacatc
    14941 ccgatgaagg tggcggtgag caggcgcaac caggtcgacc cgcacggcga cctctggatg
    15001 gcggtgctgg agtccaccgg gcagccggtg cgcttcgcct gacggacgcg cacatcccga
    15061 tcgtcgtggg taccccggag gaatgactgc tcccgaggac tttccccgca ccgcgatcgt
    15121 cactggctcc gactccggca tcgggcgcgc gacggcggtc gcgctcgccg cggcgggctg
    15181 cgacgtcggc atcacctggc actccgacga ggccggtgcg caggacaccg cggacgaggt
    15241 gcgcgcccac ggacggaagg ccgagatccg gcagctcgac ctcaccgcgc tgcccggcgc
    15301 agccgccgtc gtcgacgacc tggccgatgc gctcggcggc gtcgacgtgc tggtcaacaa
    15361 cgccggcacg ggtacgcgca ccctgctgct ggacatcgac tacgagacct ggcggtcggt
    15421 gctcgcagtt gatctcgacg gcgcgttcct gtgcctgcag cgcgccgctc gtcgcatgat
    15481 cgacgtcggg cgcggtggcc ggatcgtgaa catcacgagc gtgcacgagc accagccgcg
    15541 cgtgggtgcc gggccctact gcgccgccaa gggcgggctc gggctgctca ccaaggtcgc
    15601 tgcgctggag ctcgccgagc acggcatcac ggtcaacgcg gtcgccccgg gggagatcgc
    15661 gacgccgatg acgggtcagg aggacgagga cccgcggttg gtcgtccgcc ccggcgtgcc
    15721 gctgcgccgg cccggtgacg cccgggaggt ggccgccgtc gtggcgttcc tcgcgtcgtc
    15781 ggccgcgtcg tacgtgaccg gcgcgtcctg gccggtggac ggcgggatgc tccagatggg
    15841 cccgatggcg ggctcgcacc tgacctcgga cgactggcgc cggccgtagc gcggagtcgg
    15901 cgagcagacc ctgcgcccgc gatactgatc acgtgcacgg gctcgcgatg cggttcctcg
    15961 gacattccac ggtgcggatg gagctggccg ggcgggtggt gctcaccgat ccggttctga
    16021 ccagcgggat cgggccactg cgccgagtcg ggccgctccc ggcgcccggc gaccacgccg
    16081 acgtcgacct cgtcctgatc tcgcacctgc acggcgacca cctgcacctg ccgtcgctgc
    16141 gccggttgcc gcgcagcgcg cgggtggtgg ttccgcgcgg cgcggggggc tggctgcgcg
    16201 cgcgggggat cgcgcgggtg gaggagctcg cccccggcga gcatctggat gacggcgacc
    16261 tgcgcgtcac cggagttccc gctacgcaca gcggccaccg ctggggcccg cgttccacgc
    16321 gcggcccgca ggcgcaggcg atggggcacc tgctcgcgag cccggacgtc cgggtctacg
    16381 ccgcaggcga caccgacctg ttcccgggta tggccgcgct cgccgggcag ccgctcgacg
    16441 tcgcgctgct gccggtgtgg ggctgggggc cgacactcgg ggctgggcac ctcgacccgg
    16501 cccgagccgc tgcagcggtg gagctgctgc gcccccgggt ggcggtgccg gtgcactggg
    16561 ggaccctcgc cctggctggg ctggcccgcg ttcccggccg gccgggggcg aggatgcgca
    16621 ggctgctcgt cgagccgccg cgtgagttcg ccgcggcggt ggtggctgcg ggcgggacca
    16681 ccagggtcgc ggtcacggag ccgggtgcgg cggtcgtcct gccggccccg gcggccgact
    16741 cggagatgtc gtgatcgcgc tgtccggcag ctggaccgac gcggcctcca tcgggtaccc
    16801 ggctctgttc ggcggggtgc tgctcggatc gctggtcccg gtcgtgccga ccggtgccgt
    16861 ggtcgggg
//
LOCUS       DASRSC010000333        14783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2105239,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000333 DASRSC010000000
VERSION     DASRSC010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 14783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14783
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105239"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VFE39_16210"
     CDS             complement(<1..834)
                     /locus_tag="VFE39_16210"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HET6260181.1"
                     /translation="MDVTWLRSAALGVGVIAHGARIVAVRTPDREGRWADVALGMADE
                     DAYRRDRAYLGACVGRYANRIAGGTFTLDGREHRIPLNEPATALHGGPGGFDAVLWKR
                     EDPRDASVTLRRTSPEGENGFPGTVEVAVTYAVEGAELRIEHEARTDAPTVINMTNHA
                     YWNLAGAGSVEDHEVQLTASRFLPVDEHLIPSGPPAAVAGTPFDLRAPVRIGARLREG
                     HPQLQATRGYDHTYVIDGEPGTDGLRHAARVVEPRTGRTLELRTDQPGVQFYTGNMLD
                     GS"
     gene            904..1110
                     /locus_tag="VFE39_16215"
     CDS             904..1110
                     /locus_tag="VFE39_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016701093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260182.1"
                     /translation="MSDEQWFYCLKHGTVEPAEACRAADRLGPYPDRETAARALDIAR
                     ERTEAADRADREWAERGARDPEGD"
     gene            complement(1124..1492)
                     /locus_tag="VFE39_16220"
     CDS             complement(1124..1492)
                     /locus_tag="VFE39_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HET6260183.1"
                     /translation="MGYQLLADLVMLAHFGFLLYVALGGFLAWCRRWLIVPHAAAVAW
                     GLLSATVGMACPLTAWEDEARRRAGEQGLPRGFVDTYLTGVVYPAGSLVAAQVLVAAL
                     VLVSWVGVAHRRRRRTELPS"
     gene            complement(1519..3168)
                     /locus_tag="VFE39_16225"
     CDS             complement(1519..3168)
                     /locus_tag="VFE39_16225"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HET6260184.1"
                     /translation="MGGQHRKGGPRRWPVVAFTAVLVVSAGGVIGVRALTSCRPVDMT
                     IAADPAVAEPVRQALEGEGAVRCVTVRVVEASATQVDDIRSGATGLPVVWIPDSSLAL
                     GDLDTASGSIVDQGPSLASSPVVLVVPRADARRYGQIEQPISWNTLLTHPQPPALPDP
                     RTDRAGLAALTALHTAIGDEGSRQRPGFVNAVLALSRGSLHSAAEGYARIDRDGGSAR
                     AFFASEQSVVRRMRAGATGKGGAPVEAIALREAAAALDFPFVRIHTRQPDGLADAVDA
                     VEAQLRGPAGRQAFAAAGLRAPDGTRLPGPGGRESGWLSTRVPPAAPPPRPLAVETLR
                     VWSSLTLRSQVLAAIDVSGSMAARAGNSDRITLTSAAAQSGLDLFPDTASVGLWAFSS
                     RPPPRTPWQELVPIGPLTEPVAGAASRRAALTAATSTLPDRLGGGTALYDTVVAATRA
                     VRTDYEQGSSNTVVLVTDGDDEASDKTDLPTALQTLVAEADPARPVPVIAIGLGPEAD
                     GEALRQIAQATGGRFYEADDPQDISGVIFDALSERGCRPGC"
     gene            complement(3186..3602)
                     /locus_tag="VFE39_16230"
     CDS             complement(3186..3602)
                     /locus_tag="VFE39_16230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260185.1"
                     /translation="MQPFSPRDCSRITGVRMTKRAKAAISIGSPSSTSVGEREPQMHD
                     GPAAQRLTVDDLGHERFGWLHTFTADRQLRQFTDRRGTRARAVQVTMSQHVPYLVVDD
                     IAPVTGEALSTTTLRDRVGPEWRVAHRVVRPVSYRQ"
     gene            complement(3624..4409)
                     /locus_tag="VFE39_16235"
     CDS             complement(3624..4409)
                     /locus_tag="VFE39_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET6260186.1"
                     /translation="MTSRPGRRHDHDAGGDPELLAAKAALRDEVWAALTEAGVARFPG
                     ARNRISNFTGAEAAAERLRETDSWRAAEAVKSNPDSAQLPVRQRALEDGKTVYMAVPR
                     LAEPEPFFLLDPDHLADPPRKAASIAGASRSARRVTVAELSPVQLVVTGCVAVGEDGA
                     RLGKGGGFADLEFALATAAGLITPETVVVTTVHELQVRPAGSVPTAAHDAPVDLVVTP
                     ERVVDCRGTRGDRPVDGIRWAELTEEKIAAIPLLRALSPRHRG"
     gene            complement(4406..5215)
                     /locus_tag="VFE39_16240"
     CDS             complement(4406..5215)
                     /locus_tag="VFE39_16240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6260187.1"
                     /translation="MSPNSSVAVVTGAARGFGREIARRLLARGHRVIITDLDADAVTA
                     TAAELAASGGDVTAIVADARSAADHRRVAAVAAELGPVTVWVNNAGVLRSGRAWGQPE
                     QDQVLMVDVNVMGVVHGSRAAVELMREHGGHLLNIASMSAHGPVPGLAVYAATKSAVL
                     TFSTSLQGDLDLARVPIRVHALCPDAADTALVRDEQASPDVAILFSQRTLLTPGAVAD
                     AAVALLDGRRVVRSLPTHRAGLMRLGSLLPRVALPVLALLRSRGDRRRIRS"
     gene            complement(5286..6890)
                     /locus_tag="VFE39_16245"
     CDS             complement(5286..6890)
                     /locus_tag="VFE39_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015802478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HET6260188.1"
                     /translation="MRLTQTSDLWWKNAVVYCLDIETYADGNGDGCGDIRGLIQQIDH
                     LDRLGVTCLWLMPFFPTPDRDDGYDITDFYGVDERLGTLGDMVELVRTARDRGIRVIA
                     DLVVNHTSAQHPWFRSARSSPDSPHRDWYVWQDEPPAEGPQGVVFPDQETSLWEYDKK
                     AKQYYLHRFYKHQPDLNVANPAVRDEVARIMGFWMQLGLAGFRVDAVPFFLETDGQDD
                     ATALPDPHDYLADLSAFLRRRNGEAVLLGEVNLPYPDTMPFFGTNDGGGSTDELTMCF
                     DFIGMQQMYLALARADAEPLAETLRQRPDPPEDGHWATFVRNHDELTLDKLTDGQRTE
                     VFDAFGPDEDMQLYGRGLRRRLPPMLDGDQRRIRMVYSLLFSLPGTPVLYYGEEIGMG
                     EDLSLPGRFAVRSDMDWTAVQEQQADPDSLLSWMRLLVDRYRDCPELAWGRYTMLDPG
                     REARPVLAHRSGTDGTTVVALHNFADAEVEAVPVLQGLAGAELTDVLDPRADPVRVGD
                     DGTISVTLPPYGYRWLRCAPERVARS"
     gene            complement(6887..7852)
                     /locus_tag="VFE39_16250"
     CDS             complement(6887..7852)
                     /locus_tag="VFE39_16250"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015102202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03885 family FMN-dependent LLM class
                     oxidoreductase"
                     /protein_id="HET6260189.1"
                     /translation="MTLFGFHASHEQIHPSTLLAAVRRAEDAGFDAAMSSDHISPWSA
                     RQGQSAFAWSWLGAALQATSLPFGVVNAPGQRYHPAIIAQAIGTLGAMYPGRFWAALG
                     TGEASNEHITGDGWPRKDVRNARLRECVDVIRALLRGEEVSHDGLVTVDRARVWTLPE
                     EPPPLVGAAVSAATARWCAVWADGLITINQPVGTLREIIGAYRDAGGRGRLHLQVHLC
                     WDPDERTAEAIAMDQWRSNVFPPPACWDIDSAKVFDAVSEHVPLELVRNSVMISSDLG
                     LHTAQLAEYAALGFDEIALHHVGQEQDGFVDAFGAKVLPQLRGAA"
     gene            complement(7930..8487)
                     /locus_tag="VFE39_16255"
     CDS             complement(7930..8487)
                     /locus_tag="VFE39_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003094607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6260190.1"
                     /translation="MPNAPAPEDRDPASDEVDYWSFIDIAVERTVRTLPAVDTRAMRL
                     VLSLYRASNAMVYDLESAIHRPRGWSWAGFRVLFVLWLAGPLEAKRVAQLAGMSRAAV
                     SALVNTLERDGLVSRRKAGHDRRTVELSLGDAGEHLVAAAFEAHNERERTWASALSDD
                     EVVQLTALLEKLMASNVAAAAKRRR"
     gene            complement(8624..9886)
                     /locus_tag="VFE39_16260"
     CDS             complement(8624..9886)
                     /locus_tag="VFE39_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HET6260191.1"
                     /translation="MTAETLDLALRAAELDATDPLAAHRDRFVPSPDVVAYLDGNSLG
                     RPLAGTAERLDRFVREEWGGGLIRAWTGDGPAGSAQPWMGWPGWVGDRIAAAALGAAP
                     GQTVVADSTTVLLYKLARAAVDAQARDGRTEIVLDTDNFPTDRYVLEGIAAERGCVLR
                     WIEPDPAEGVTPEQVAAVVGPTTGLVLFSHVAYRSGWLADAAAITRIVHDAGGLMLWD
                     LCHSAGSVQLELDAWGVDLAVGCTYKYLNGGPGSPAFGYVRRDLQDVLRQPIQGWMGR
                     RDPFLMGPGYTPAAGVRGVVSGTPPILAMVPLLSALDQLDEAGIAAVRAKSVALTEFA
                     VELVDAWLAPHGVEVASPRDPARRGGHVTIRRAGFHSVLDELWRRGVIPDFREPDGIR
                     IGLAPLSTSFAEVHAGLAVLRDVLARPG"
     gene            complement(9883..10761)
                     /locus_tag="VFE39_16265"
     CDS             complement(9883..10761)
                     /locus_tag="VFE39_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HET6260192.1"
                     /translation="MTDAGGTRPLEDGIVREFGVESSPDGRVNLSYGAYLHLDELLSA
                     QHPVSRPVHHDELLFIVQHQTSELWLKLVLHELCAVQAHLAADDLRPALKGLARVKHI
                     QRTLTDQWSVLATLTPSEYAEFRSFLGTSSGFQSDQYRAVEFVLGNKNAAMLELFGHD
                     ERARAQLAELLGRPSVYDEFLRYLARHGHDVPRELLERDVTQPHTFTPELVPVFRRIY
                     EDSHEHWEAYEACEELVDLEENFQLWRFRHLKTVERTIGVKRGTGGSSGVAFLRAALD
                     LTFFPELYAVRTEIGG"
     gene            complement(10825..11274)
                     /locus_tag="VFE39_16270"
     CDS             complement(10825..11274)
                     /locus_tag="VFE39_16270"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HET6260193.1"
                     /translation="MAFQVTEVQRFLKGADYPMDGKALAELAKKNGAGDDLVGALRDI
                     GKVDGPNEVMKQLKGELGGSTPGGDKSAEKSYKDVRRPAFQVNEVQKYLKGADYPMDG
                     KELAELAKKNGAGDDLVGALQGIGKVDGPNAVMKELKDHLGGRPGDA"
     gene            complement(11347..12594)
                     /locus_tag="VFE39_16275"
     CDS             complement(11347..12594)
                     /locus_tag="VFE39_16275"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6260194.1"
                     /translation="MLHPPAPRERPTPPTLCRATADDLTAMATADGRGFGMHYTDTDI
                     EDFRPLFDPDRFLLAQDPADGAKQDGPILGITGSFPFDVTLPGGTTLPAPGVTWVSVA
                     VTHRRQGILRAMMEEQHRGFVADASAVSLLTASEGAIYGRFGYGIATVHRSVEITRRR
                     ATFRPGAPDPGGIRQVDAHEARLLLPEIHRRWAALVPGALSRSDRWWDNHMLDRESRR
                     GGATALFYLVHEDGYASYRIEHGERTCQVAELVSVTEEAHAALWRVLLALDLVETVAR
                     WSHPLDDPLQYLLADPRQVRTTRIRDGMWARILDVPAVLSARRYAAELDVVLDVHDPF
                     LGLGGRFRLRGGPDGASCERVDAGSPTVAVDVTVLGSLLFGGERAETLARAGLLETDD
                     PAALRRVDTAFLADRLPQHGTEF"
     gene            complement(12648..13754)
                     /locus_tag="VFE39_16280"
     CDS             complement(12648..13754)
                     /locus_tag="VFE39_16280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET6260195.1"
                     /translation="MKIAHLVPTLHPGGPEIGLVDLAGAARETGLELVVVALASTSET
                     SQVSALQRLGVPVAELGLAPWDPRAVPRIAGLLRERKVQLVHTHLPPADVVGAAAAVR
                     NRIPAVSTLHRIENLPADRLDRLKRTAKILARQRFMTRTIAISQVQREWYRGLSGSEK
                     NLVLVPNGVADPGPLDAAERHRRRAALGVGDGDVLAVSTSPMRRDQGHELLLDALESL
                     PDGLPLVVALAGDGPLRPWLESRVDGSDDLADRVRFVHRQQEPTALLGAADLILHTGR
                     SGAAPTALLRAMAAGVPAVATRVGGVPEIVTAGNGVLVPLDPGAIADALVALTEDGAR
                     RERLGKAARARFLAEYEAVGWAGRLRDLYQTVLS"
     gene            13922..>14783
                     /locus_tag="VFE39_16285"
     CDS             13922..>14783
                     /locus_tag="VFE39_16285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260196.1"
                     /translation="MDVRPVGLQDDDTPVIMLVDAATAVERGLVEDWMRQEELRPVAV
                     LPVKGPALAQPLVDSGEDAVVAAVRVAWLPRERNGQRRVRWADVVSLRNPRHPPASQQ
                     ARIVRREPDRVALVVAEPASVGSLRARFAASRSVERPSGAGSVRAFGDFVARQAALAL
                     DRAERALVGDRYKVPRQIVEAIEGSPEFRREVRELAASLGRPEAEVAAKAQADLHDLV
                     ASMSPMAVDLLTSALRPLHARAWDVQVDTAGLEKLRECNRRHALVFLPSHRSYADPLL
                     LADVLAEHDFP"
BASE COUNT         2027 a         5346 c         5385 g         2025 t
ORIGIN      
        1 cgacccgtcc aacatgttcc ccgtgtagaa ctggacgccc ggctggtctg tgcgcagctc
       61 gagcgtccgc ccggtgcggg gctccacgac acgcgcggcg tggcgcagtc cgtcggtacc
      121 tggctcgccg tcaatcacgt aggtgtggtc gtagccgcgg gtcgcctgca gctgtggatg
      181 gccctcgcgc agccgcgcgc cgatccgcac cggtgcgcgc aggtcgaacg gggtaccggc
      241 cacggccgcg ggcggaccgg acgggatgag gtgctcgtcc accggcagga agcgcgacgc
      301 cgtgagctgc acctcgtggt cctcgacgct gccggccccg gcgaggttcc agtacgcatg
      361 gttggtcatg ttgatcacgg tcggggcgtc ggtgcgggcc tcgtgctcga tgcgcagctc
      421 cgccccctcg accgcgtagg tgacggcgac ctccaccgtg cccggaaagc cgttctcccc
      481 ctcggggctg gtgcgccgca gcgtcaccga cgcgtcgcgg gggtcctcac gcttccagag
      541 gacggcgtcg aagccaccgg ggccaccgtg caacgcggtc gcgggctcgt tgagcgggat
      601 ccggtgctcc ctgccgtcga gggtgaacgt gcctccggcg atccggttgg cgtaccggcc
      661 cacgcaggcg cccaggtagg cacgatcgcg ccggtaggcg tcctcgtcgg ccatgccgag
      721 cgcgacgtcc gcccaccggc cctcgcggtc gggcgtgcgg accgccacga tccgggcacc
      781 gtgggcgatc acgcccaccc ccagtgcagc actgcgcagc caggtcacgt ccatggtcgg
      841 ccatgctgcc cgagccgtgg cgggacgagc gccgggggcc gcggggtgac acgctgtgga
      901 cccatgagcg acgagcaatg gttctactgc ctcaagcacg gcacggtcga gccggcggag
      961 gcctgccgcg cggccgaccg cctcggcccc taccccgacc gcgagacggc ggcgcgagca
     1021 ctcgacatcg cgcgcgagcg cacggaggcc gccgaccgcg ccgaccgcga gtgggctgag
     1081 cggggtgctc gcgatcccga gggggactga ccggccgagc gggtcaactc ggcagctcag
     1141 tgcggcgccg gcgccggtgc gcgaccccca cccacgagac cagcaccagt gccgccacca
     1201 gcacctgcgc cgcgacgagg ctgccggcgg ggtacaccac gccggtcagg taggtatcga
     1261 cgaagccgcg cggcaggccc tgctcacccg cgcggcgccg agcctcgtcc tcccaggcgg
     1321 tgagcgggca ggccataccg acggtcgcgc tgagcaggcc ccaggccacc gcggcggcgt
     1381 gcggcacgat cagccaccgg cggcaccagg cgaggaaccc acccagcgcg acgtagagca
     1441 ggaacccgaa gtgggccagc atcaccaggt cggcgagcag ctggtaaccc atccaccgag
     1501 gatgccagga gatccggttc agcagcctgg ccgacaccca cgttcggaga gcgcgtcgaa
     1561 gatgaccccg ctgatgtcct gcgggtcgtc ggcctcgtag aaccggccgc cggtggcctg
     1621 cgcgatctgg cgcagcgcct cgccgtcggc ctctggtccc aacccgatcg cgatcaccgg
     1681 aaccggccgg gccgggtcgg cctcggccac cagtgtctgc agcgcggtgg gtaggtcggt
     1741 cttgtcgctc gcctcgtcgt ccccgtcggt gacgagcacc accgtgttcg agctcccctg
     1801 ttcgtagtcg gtgcggacgg ccctggtggc ggccactacc gtgtcgtaaa gggcggtccc
     1861 gccaccgagg cggtcgggca gcgtgctggt ggcggcggtg agcgcggcgc gtcgggacgc
     1921 tgcaccggcg accggctcgg tcaacgggcc gatcggcacg agctcctgcc acggggtccg
     1981 cggcggcggg cgcgaggaga acgcccacag cccgaccgac gccgtatccg ggaacaggtc
     2041 cagaccggac tgtgccgcgg ccgaggtgag tgtgatccgg tcgctgttgc ccgcacgcgc
     2101 cgccatcgag ccggacacgt cgatcgcggc cagcacctgg ctgcgcaagg tgagcgagct
     2161 ccacacccgc agcgtctcga ccgctaacgg ccgcggtggg ggtgcagcgg gcggtacccg
     2221 ggtgctcagc cacccggact cgcggccgcc ggggccgggc agccgggtcc cgtccggtgc
     2281 cctcaggcct gcggcggcga acgcctgccg gccggccggg ccgcgcagct gggcttctac
     2341 ggcgtccacc gcgtcggcga gcccgtcggg ttgccgggtg tggatgcgga cgaacgggaa
     2401 gtccagcgcg gcggccgcct cccgcagggc aatggcctcg acgggtgcgc cgcccttccc
     2461 cgtggcaccc gcccgcatcc gccgcacgac ggactgctcg gacgcgaaga aggccctggc
     2521 gctcccgccg tcgcggtcga tgcgggcgta tccctccgca gccgagtgga gcgagccgcg
     2581 ggacagtgcg agcaccgcgt tgacgaaccc cgggcgttgc ctgctgccct cgtcgccgat
     2641 cgcggtgtgc agggccgtga gcgccgccag cccggcgcgg tcggtgcggg ggtcgggcag
     2701 ggcgggcggt tgcggatggg tgaggagcgt gttccacgag atgggctgct cgatctggcc
     2761 gtatctccgg gcatcggccc gcgggacgac cagtacgacc ggcgacgagg ccagcgacgg
     2821 cccctggtcg acgatcgagc ccgatgcggt gtccaggtct ccgagggcga gcgaggagtc
     2881 cgggatccag acgaccggca gccccgtagc gcccgagcgg atgtcgtcca cctgcgtcgc
     2941 cgacgcctcg acgacccgga cggtgacgca ccgcactgcg ccctcgccct ccagcgcctg
     3001 acgcacgggc tcggccacag cgggatcggc ggcgatggtc atgtcgaccg ggcggcagga
     3061 ggtgagtgct cggactccga tcacaccgcc tgcgctgacc accagcacgg cggtgaaagc
     3121 cacgaccggc cagcgccgcg gtccgccctt ccggtgctgg ccccccaccg cgccctcccc
     3181 ctgtgttact ggcggtagct aaccggacgg accacccgat gcgccactcg ccattcgggg
     3241 ccaactcgat cacgcagcgt agtcgtcgac agggcctcac cggttaccgg cgctatgtcg
     3301 tcaaccacca ggtagggcac gtgctgggac atggtcacct ggactgccct ggcccgggtc
     3361 cccctccgat ccgtgaactg ccgaagctgc cggtccgcgg tgaacgtgtg cagccagccg
     3421 aagcgttcat gcccgaggtc gtcgactgtg agccgctgcg cggccggccc gtcgtgcatc
     3481 tgcggttcgc gttcaccaac cgaggtcgag gacgggctgc cgatcgagat ggccgccttc
     3541 gcccgcttcg tcatccggac gcccgtgatg cggctgcagt ccctcgggct gaacggctgc
     3601 acccccaccc cgtggccctg gccctatccc cgatgacggg gtgacagggc ccgaagcagc
     3661 ggtatcgcgg cgatcttctc ctcggtcagt tcggcccagc ggatcccgtc gacgggccgg
     3721 tcaccccgcg taccgcggca gtccactacg cgctccggcg tcacgacgag gtcgacgggt
     3781 gcgtcgtgcg ccgccgtcgg cacgctgccg gcggggcgca cctgcaactc gtgcaccgtg
     3841 gtgacgacga ccgtctcggg ggtgatcagg ccggccgcgg tggcgagcgc gaactccagg
     3901 tccgcgaacc cgccgccctt gcccagccgg gcaccgtcct ccccgaccgc gacgcacccg
     3961 gtcaccacga gctgcacggg tgacagctcc gccacagtta cccggcgcgc cgatcgcgag
     4021 gcgcccgcga tcgacgccgc cttccgcggc gggtcggcaa ggtgatcggg gtcgagcagg
     4081 aagaacggct ccggctcggc cagccgcggc acggccatgt agacggtctt gccgtcctcg
     4141 agcgcacgct gccgcaccgg gagctgagcg gagtccgggt tcgacttgac ggcctctgcg
     4201 gcccgccacg agtcggtctc gcgcagccgc tccgcggcgg cctccgcccc ggtgaagttg
     4261 gagatccggt tgcgggcacc ggggaagcgc gccaccccgg cctcggtgag cgccgcccac
     4321 acctcgtccc gcagggccgc cttcgccgcc aacagctccg gatcgcctcc ggcgtcgtgg
     4381 tcgtggcgac ggcccggcct gctcgtcacg agcggatccg gcgccggtcg ccgcggctcc
     4441 gcagcagcgc gagcacgggc agggccacgc gcgggagcag cgagccgagc cgcatgagcc
     4501 ccgcccggtg ggtgggcagg gagcggacca cgcgacgtcc gtcgagcagc gcgaccgcgg
     4561 cgtcggccac cgcgccgggg gtgagcaggg ttcgctgcga gaacaggatc gcgacatcgg
     4621 ggctcgcctg ctcgtcgcgc accagcgcgg tgtcggcggc gtcggggcac aacgcatgaa
     4681 cccggatcgg cacgcgggcg aggtcgagat cgccttgcag gctggtactg aaggtcaaca
     4741 cggccgactt cgtggcggcg tacaccgcaa gtccgggcac cggcccgtgc gccgacatcg
     4801 acgcgatgtt gagcagatgc ccgccgtgct cgcgcatcag ctcgaccgcg gcccgcgaac
     4861 cgtggaccac gcccatgaca ttcacgtcga ccatcagcac ctggtcctgt tccggctgcc
     4921 cccacgcccg tccggagcgc aacacacccg cgttgttcac ccacaccgtc accggcccca
     4981 gctcggcggc cacggcggcg acgcggcggt ggtcggccgc gctgcgggcg tcggcgacga
     5041 tcgcggtgac gtcgccaccg gaggccgcca gctccgctgc ggtggcggtg acggcgtcgg
     5101 catccaggtc ggtgatgatt acccggtgcc cgcgggcgag gaggcggcga gcgatctcgc
     5161 ggccgaaccc acgggcggca ccggtgacga cagcgacact gctgttgggg ctcacggccg
     5221 acactgtgtc atgggacgcc tcggtcaccg acgacccggt tgcggacgcc gaggtgggcg
     5281 cgctcctagg accgcgccac ccgctccggg gcacaacgca gccaccggta cccgtagggc
     5341 ggcaaggtca ccgagatcgt cccgtcgtca ccgacccgga ccggatccgc gcgtggatcg
     5401 agcacgtcgg tgagctccgc tccggcgagc ccctgcagca ccgggacggc ctcgacctcg
     5461 gcgtcggcga agttgtgcag cgcgacgacc gtggtgccgt cggtgccgct gcggtgcgcg
     5521 agcaccggcc gggcctcccg tccggggtcg agcatggtgt agcggcccca cgcgagctcc
     5581 gggcagtccc ggtacctgtc gacgagcagc cgcatccagg acagcaacga gtcggggtcg
     5641 gcctgctgct cctgcaccgc cgtccagtcc atgtccgagc gcaccgcgaa ccggccgggc
     5701 aggctcaggt cctcacccat accgatctcc tcgccgtagt agagcaccgg cgttccgggc
     5761 agggagaaga gcaggctgta gaccatccgg atccggcgct ggtccccgtc gagcatcggc
     5821 ggcaggcgcc ggcgcaggcc gcggccgtag agctgcatgt cctcgtcggg cccgaaggcg
     5881 tcgaagacct ccgtgcgttg gccgtcggtg agcttgtcga gcgtcagctc gtcgtggttg
     5941 cgcacgaacg tggcccagtg cccgtcctct ggcgggtcgg gccgctgtcg cagcgtctcg
     6001 gccagcggtt cggcgtcggc ccgcgccaga gcaaggtaca tctgctgcat gccgatgaag
     6061 tcgaagcaca tcgtgagctc gtcggtcgag cccccgccgt cgttcgtgcc gaagaacggc
     6121 atcgtgtccg ggtagggcag gttgacctcg ccgaggagca cggcctcgcc gttgcgcctg
     6181 cggaggaacg cgctcaggtc ggcgaggtag tcgtgcgggt cggggagcgc ggtggcgtcg
     6241 tcctggccgt cggtctccag gaagaacggc acggcgtcga cgcggaagcc agccaggccc
     6301 agctgcatcc agaagcccat gatccgcgcg acctcgtcgc gcaccgccgg gttggccacg
     6361 ttcaggtcgg gctgatgctt gtagaaccgg tgcagatagt actgcttcgc cttcttgtcg
     6421 tactcccaga ggctggtctc ctggtccggg aacaccacgc cctgcgggcc ctcggcaggc
     6481 ggctcgtcct gccagacgta ccagtcgcgg tggggggagt cggggctgct gcgcgcgctc
     6541 cggaaccacg ggtgttgggc ggacgtgtgg ttgaccacga ggtcggcgat cacccgaatc
     6601 ccgcggtccc gggcagtgcg gaccagctcc accatgtcgc cgagcgtgcc gagccgttcg
     6661 tcgaccccgt agaagtcggt gatgtcgtag ccgtcgtcgc ggtcgggtgt ggggaagaac
     6721 ggcatcagcc acaggcaggt gacgccgagc cggtcgaggt ggtcgatctg ctggatgagg
     6781 ccgcggatgt caccgcagcc gtcgccgttg ccgtcggcgt aggtctcgat gtcgaggcag
     6841 tagacgacgg cgttcttcca ccacaggtcg gatgtctgcg tgagtctcat gccgcacccc
     6901 gcagctgggg gaggaccttg gccccgaacg cgtcgacgaa cccgtcctgc tcctggccga
     6961 cgtggtgcag cgcgatctcg tcgaagccca gcgcggcgta ctccgcgagc tgcgcggtgt
     7021 gcaggccgag gtcgctcgag atcatcaccg agttgcgtac cagctccagt gggacatgct
     7081 ccgacacggc gtcgaacacc ttcgccgagt cgatgtccca gcaggcgggc ggcgggaaga
     7141 cgttgctgcg ccactggtcc atcgcgatcg cctcggccgt ccgctcgtcg gggtcccagc
     7201 agaggtggac ctgcaggtgc agccggccgc gcccgccggc gtcccgatag gcgccgatga
     7261 tctcgcgaag cgtcccgacg ggctggttga tcgtgatgag gccgtcggcc cagacggcgc
     7321 accaccgcgc cgtcgcggcg ctcaccgccg caccgaccag cggcggcggt tcctccggca
     7381 gggtccagac acgggccctg tcgacggtga cgagcccgtc gtggctcacc tcctcgccac
     7441 gcagcagggc cctgatcacg tcgacgcact cgcgcagccg ggcgttgcgc acgtccttgc
     7501 ggggccaccc gtccccggtg atgtgctcgt tgctcgcctc accggtgccc aaggcagccc
     7561 agaaccggcc ggggtacatc gcgccgagcg ttccgatcgc ctgcgcgatg atcgccgggt
     7621 gataacgctg gccgggtgcg ttgaccacgc cgaacggcaa cgacgttgcc tgcagcgccg
     7681 ccccgagcca ggaccaggcg aatgcggact ggccctgccg cgcactccag ggggagatgt
     7741 ggtccgacga catcgcggcg tcgaatccgg cgtcctcggc tcggcggacc gcggccaaca
     7801 gggtgctggg atggatctgc tcgtgcgagg catggaagcc gaatagcgtc attccgtcct
     7861 ctacccccaa aagggggctc cggaatcgcc tgcttcgccc gatttgccgg ccgaacctcg
     7921 ccgcccaggt cagcggcgtc gcttcgccgc cgccgcgaca ttgctcgcca tgagcttctc
     7981 caacagtgcc gtgagctgga cgacctcgtc gtcggacagg gcggacgccc acgtccgctc
     8041 ccgctcgttg tgcgcctcga aggcagccgc gaccaggtgc tcgcccgcat cgccgaggga
     8101 cagctccacg gttcgccggt cgtggcccgc cttccggcgg gacaccaggc cgtcccgctc
     8161 cagggtattg accagcgccg acaccgccgc ccggctcatt cccgccagct gagccacccg
     8221 ctttgcctcg agcggcccgg ccagccagag cacgaacagc acccggaacc ccgcccacga
     8281 ccagccacgg gggcggtgga tcgccgactc caggtcgtag accatcgcgt tcgacgcgcg
     8341 gtagaggctg agcaccaacc gcatggcacg cgtgtccacc gcggggaggg tgcgaacggt
     8401 ccgctcgacc gcgatgtcga tgaacgacca gtagtcgacc tcgtcggacg ccgggtcgcg
     8461 gtcctcggga gccggagcgt tgggcacagg gcgcagcgta ggcggctcgc cccgtgacca
     8521 ggggaagcac cgccacggta ggccgcacag tcgcggggtg gttgatcgcc gcgagatcga
     8581 ggctccggcc ctccgcaggg ccagaacccg gatctcgccc cgatcagccc ggccgcgcca
     8641 gcacgtcccg gagcacagcg agcccggcgt ggacctcggc gaagctcgtg ctcagcgggg
     8701 cgaggccgat ccggatcccg tccggctcac ggaagtcggg gatcacgccg cgccgccaga
     8761 gctcgtcgag caccgagtgg aagccggccc ggcggatcgt tacgtgtccg ccccggcgcg
     8821 cggggtcgcg cggcgaggcc acctcgacgc cgtgcggggc gagccacgcg tccaccagtt
     8881 cgaccgcgaa ctccgtcagc gccacggact tggcccgtac tgcggcgatc ccggcctcgt
     8941 ccagctggtc cagggccgag agcagcggca ccatcgccag gatcgggggc gttccgctga
     9001 ccacgccgcg gacgcccgcc gcaggcgtat agccggggcc catcaggaac gggtcgcgcc
     9061 ggcccatcca cccctggatc ggctggcgca gcacgtcctg caggtcgcgc cggacgtagc
     9121 cgaacgccgg tgagccgggg ccgccgttga ggtacttgta ggtgcagccg acggccaggt
     9181 ccacgcccca cgcgtcgagc tccagctgca ccgaccccgc ggagtggcag aggtcccaga
     9241 gcatgagtcc gcctgcgtcg tgcacgatcc gagtgatcgc cgcggcgtcg gcgagccagc
     9301 ccgaccggta ggccacgtgg ctgaacagca ccagccccgt ggtcggcccc acgactgccg
     9361 cgacctgctc aggggtgacc ccctcggcgg ggtcgggctc gatccagcgc aggacgcacc
     9421 cgcgctccgc agcgatcccc tccaggacgt agcggtccgt cgggaagttg tcggtgtcga
     9481 ggacgatctc ggtgcggccg tcgcgggcct gcgcgtcgac cgcggcgcgg gcgagcttgt
     9541 agagcagcac cgtcgtcgag tcggcgacca cggtctggcc gggggcggca ccgagcgcgg
     9601 cggcggcgat ccgatcgccg acccacccgg gccagcccat ccacggttgc gccgagcccg
     9661 cgggcccgtc gcccgtccac gcgcggatga gcccgccgcc ccactcctcg cggacgaacc
     9721 ggtcgagccg ttcggcagtg ccggccagcg ggcggccgag ggagttgccg tcgagatagg
     9781 cgacgacgtc ggggctgggc acgaaacggt cgcggtgcgc ggcgagcgga tcggtggcgt
     9841 ccagctcggc cgcgcggagt gccaggtcga gtgtctcggc tgtcatccac cgatctccgt
     9901 gcgcaccgcg tacagctccg ggaagaacgt gaggtccagc gctgcccgca ggaacgcgac
     9961 gccgctggag ccacccgtgc cgcgcttcac cccgatggtg cgttcgacgg tcttgaggtg
    10021 ccggaacctc cagagctgga agttctcctc caggtcgacc agctcctcgc aggcttcgta
    10081 ggcctcccag tgctcatggg agtcctcgta gatgcgacgg aacaccggca cgagctcggg
    10141 agtgaacgtg tgcggttgcg tgacgtcacg ttccagcagc tcgcgcggta cgtcatggcc
    10201 gtggcgggca aggtagcgca ggaactcgtc gtagacgctc ggtcggccca gcagctccgc
    10261 cagctgcgcg cgggcccgct cgtcgtgccc gaacagctcc agcatcgcgg cgttcttgtt
    10321 gccgagcacg aactccaccg cgcggtactg gtcggactgg aagcccgacg aggtgccgag
    10381 gaagctccgg aactccgcgt actccgacgg ggtgagggtg gccagcaccg accactggtc
    10441 ggtcagggtg cgctggatgt gcttgaccct ggccagcccc ttgagcgccg ggcgcaggtc
    10501 gtcggccgcg agatgggcct gcaccgcgca cagctcgtgc aacaccagct tcagccacag
    10561 ctcggacgtc tggtgctgca cgatgaacag cagctcatcg tggtgcaccg gcctgctcac
    10621 cgggtgctgc gccgacagca gctcgtcgag gtgcaggtac gcgccgtagg aaaggttcac
    10681 ccggccatcg ggcgagctct cgacgccgaa ctcgcggacg atgccgtcct cgagcgggcg
    10741 ggttcccccg gcgtcggtca tgcccgcact gtagggccgt cagcgcgcag gcccccctgc
    10801 acggtgccac cgggcggcgg cccgtcaggc gtcgccgggt ctgcccccga ggtggtcctt
    10861 gagctccttc atcacggcgt tcggaccgtc gaccttgccg atgccctgca gggccccgac
    10921 gaggtcgtcg cctgcgccgt tcttcttggc cagctcggcg agctccttgc cgtccatcgg
    10981 gtagtcggcg cccttgaggt acttctgcac ctcgttgacc tggaacgccg ggcgccggac
    11041 gtccttgtag ctcttctcag cggacttgtc gccgcccggg gtggagccgc ccagctcgcc
    11101 cttcagctgc ttcatcacct cgttcggacc gtccaccttg ccgatgtccc gcagcgcccc
    11161 gacgaggtcg tcgcctgcgc cgttcttctt ggcgagctcg gcgagggcct tgccgtccat
    11221 cgggtagtcg gcacccttga ggaagcgctg cacttcggtc acctggaaag ccatgagccg
    11281 gggctacccg atggccacgc cgatatgcgg cccgctcccc tcggggggtt ggcgctgccc
    11341 cgagcctcag aactccgtgc cgtgctgcgg taggcggtcg gccaggaacg cggtgtcgac
    11401 gcggcgcagc gcggcggggt cgtccgtctc gagcagcccg gcgcgcgcca gggtctcggc
    11461 ccgttcgccg ccgaacaaca gcgagcccag caccgtgacg tcgaccgcga cggtcggact
    11521 gcccgcgtcc acgcgctcgc agcttgcgcc gtccggcccg ccgcgcagcc ggaaccgccc
    11581 gcccaggccg aggaacgggt cgtgcacgtc gaggacgacg tcgagctcgg cggcataccg
    11641 gcgtgccgac agcactgcgg gcacgtcgag gatccgcgcc cacatcccgt cccggatacg
    11701 ggtggtgcgc acctgccgtg gatcggcgag caggtactgc agcgggtcgt cgagcggatg
    11761 ggaccaacgg gcgaccgtct ccacgaggtc gagggcgagc agtacccgcc acagcgcggc
    11821 gtgcgcctcc tcggtgacac tcacgagctc agcgacctgg caggtccgct ccccgtgctc
    11881 gatccggtag gaggcgtagc cgtcctcgtg cacgaggtag aagagcgccg tggcgccgcc
    11941 gcggcgtgac tcgcggtcga gcatgtggtt gtcccaccac ctgtcgctgc ggctgagtgc
    12001 gcccggcacg agggcggccc atcggcggtg gatctcgggg aggagcagtc gggcctcatg
    12061 ggcgtcgacc tggcggatgc cgcccggatc gggagctccg ggacggaacg tggcgcggcg
    12121 gcgggtgatc tccacggagc ggtgcacggt ggcgatgccg tagccgaagc ggccgtagat
    12181 cgcgccctcg ctggccgtga ggagcgagac cgcggaggcg tcggccacga aaccgcggtg
    12241 ctgctcctcc atcatcgcgc gcaggatgcc ttggcggcgg tgggtcaccg ccacggacac
    12301 ccacgtcacg cccggggcgg gcagcgtggt gccgccgggc agggtgacgt cgaatgggaa
    12361 cgagccggtg atcccgagga tcggcccgtc ctgcttcgcc ccgtccgcgg ggtcctgggc
    12421 caacaggaag cggtcggggt cgaacagcgg tcggaagtcc tcgatgtccg tgtcggtgta
    12481 gtgcatgccg aacccgcggc cgtccgccgt ggccatggcg gtgaggtcgt cggcggtggc
    12541 gcgacacagc gtgggcggcg tggggcgttc gcggggcgca ggtgggtgca gcacggtcgc
    12601 ggattctgac ggatgcggca cgtcgcgtcg acgggtatgt ggcccgatca gctcagcacg
    12661 gtctggtaga ggtcccgcag ccgcccggcc cacccgacag cctcgtactc ggcgaggaac
    12721 cgggcgcgcg cggccttgcc gagccgctcc cggcgtgcgc cgtcctcggt gagcgcgacg
    12781 agcgcgtcgg cgatggcgcc ggggtcgagc ggcacgagca caccgttgcc ggccgtgacg
    12841 atctccggga cacccccgac ccgagtggcg acggcgggca ccccggcggc catcgcccgc
    12901 agcaacgccg tcggtgcggc gccggaacgg ccggtgtgca ggatcaggtc ggccgccccg
    12961 agcagcgccg tgggttcctg ctggcggtgc acgaagcgga cgcgatcggc caggtcgtcg
    13021 ctcccgtcga cgcgcgactc cagccatggc cgcagcggcc cgtcgccggc cagcgcgacc
    13081 accagcggaa gcccgtccgg cagcgactcg agggcgtcga gcaggagctc gtggccctgg
    13141 tcgcggcgca tcggggacgt actcaccgcg agcacgtcgc cgtcgccgac ccccagggcg
    13201 gcccggcgac gatgccgctc ggcggcgtcg agggggccgg ggtcggccac cccgttcggg
    13261 acgaggacca ggttcttctc ggaccccgac aggccgcggt accactcccg ctgcacctgc
    13321 gagatggcga tcgtccgggt catgaaccgc tgccgggcga ggatcttcgc ggtgcgtttg
    13381 agccggtcga gccggtcggc cggcaggttc tcgatgcggt gcagggtgga cacggccggt
    13441 atccggttgc ggacggctgc cgcggccccc accacgtcgg ccggcggcag gtgcgtatgc
    13501 acgagctgca ccttccgctc ccgcagcagc cccgcgatcc ggggcaccgc acgcggatcc
    13561 cacggggcga gccccagctc cgcgaccggg actccgagcc gctgcagcgc gctgacctgg
    13621 ctcgtctccg atgtggacgc gagcgcgacc accacgagct ccaggcccgt ctcccgcgcc
    13681 gccccggcga ggtcgaccag cccgatctcg ggtccgccag ggtggagagt cgggacgagg
    13741 tgcgcgatct tcacatgccc tccgggtgac gtcgacggcc cccgcagggc gcggcggccg
    13801 tgcacgcccg gccactctgc gtgcgtgcgg gcgccgacac taccgctgcc gctcgactcg
    13861 gcgtgatggt cacatcgtga ccggggccgg gcaggatgat gcgtcgacgg ggaggtgggg
    13921 aatggacgtg cgtccggtcg gcctgcagga cgatgacacc ccggtgatca tgctcgtcga
    13981 cgcggccacg gcggtggagc gtgggctcgt cgaggactgg atgcggcagg aggagctgcg
    14041 cccggtggcg gtcctgccgg tcaagggccc ggcgttggcc cagccgctgg tcgactccgg
    14101 tgaggacgcc gtcgtcgctg cggtacgggt ggcgtggctg ccccgcgagc gcaacgggca
    14161 acgccgggtt cgctgggccg acgtcgtgtc cttgcgcaat ccgcggcacc caccggcgag
    14221 ccagcaggcc cggatcgtgc ggcgggagcc ggaccgggtg gcgctcgtgg tggccgagcc
    14281 cgccagcgtg gggtcgctgc gggcccggtt cgcggcgagc cgttccgtcg agcggccctc
    14341 cggcgcgggg tcggtgcggg cgttcggtga cttcgtcgcc cggcaggccg cgctggcgct
    14401 cgaccgggct gagcgcgccc tggtgggtga ccgctacaag gtgccccggc agatcgtcga
    14461 ggcgatcgag ggtagccccg agttccgccg tgaggtcagg gagctcgccg cctcgctggg
    14521 gcgtcccgag gcggaggtgg ccgcgaaggc gcaggccgac ctgcacgacc tcgtcgcctc
    14581 gatgagcccg atggcggtcg acctgctcac gtccgcgctg cggccgctgc acgccagggc
    14641 gtgggacgtc caggtggaca ccgccgggct ggagaagctg cgcgagtgca accggcggca
    14701 tgcgctcgtg ttcctgccca gtcaccgatc ctacgccgac cccctgctcc tggccgatgt
    14761 gctcgccgag cacgatttcc ccc
//
LOCUS       DASRSC010000334         4050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2105653,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000334 DASRSC010000000
VERSION     DASRSC010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2105653"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..1528
                     /gene="zwf"
                     /locus_tag="VFE39_16290"
     CDS             152..1528
                     /gene="zwf"
                     /locus_tag="VFE39_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET6260197.1"
                     /translation="MAADPVGALVIFGATGDLAKLETFPALVGLVDRGVLTVPVIGVA
                     RSGWNLDRFREYAAQSLRLNGMDPEAPSARTMLGLLRYVDGDLDDSATYEAMSREMGD
                     CRRALYYLEVPPVLFGRIAEGIAAAGRADGARVMVEKPFGTDLASAIALDQTMHDHFP
                     EDAIYRVDHWLGLDPLNDVLVARFANSMFEPLLDRDHVESIQITMAEAFDVADRGRLY
                     DHTGAIRDVVQNHMLQVLATVIADPPDGSGMDSWLDAKSRVISALRPLTPADAVRGQY
                     DGYRDVEGVDPVSTVETYVAVRLALDSWRWAGVPILIRAGKTMPVTATEVSIRFRPVP
                     FDVFGISRTRMHNTLRFRIWPNTEIGLALAGKKPGAGREPQMQDLVFAEDSASDMRPY
                     DRLIGAALDGNPVLFARQDTVEAAWRVVDPVLGDAVPVQGYQRGTWGPKEADSLLPNG
                     DDWHDPVA"
     gene            1610..2974
                     /locus_tag="VFE39_16295"
     CDS             1610..2974
                     /locus_tag="VFE39_16295"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6260198.1"
                     /translation="MIVGGGFGGLFAARALRHRDVAITLVDRAQHHLFQPLLYQVATG
                     ILSEGQIAAPLRDVLKRHRNVECVLADVVDVDAAGRRVVAVRPGGDRMTLPYDDLVVA
                     AGVRQSYYGHDEFARWAPGMKTVADALAIRRRVFGAFELAETATDPDEQRRWLTFAVV
                     GAGPTGVELAGQIREVATRTLRKEFRHIRPEDARVLLFDGGSAPLASFGTALSAKAAA
                     ALRDLGVELHFGSIVTDVDGEGLQVRDRAGQSICHGAGTVIWAAGVEAPPVAEALAVA
                     TSARRDRAGRICVEPDLTIRGHPEISVVGDMMSLDALPGVAEVAMQSGLYAGHRIRQR
                     LRDRTGNGNGHGTGKGIAAFRYYDLGSAAYLSRGNAVVSAGPVRFAGFGGWLAWLFIH
                     LAFLTGYRNRLGAVITWAAAFIRDARRERAFTTQQIERLQDVYEGPPRKDRPAVGPSP
                     PHPP"
     gene            2988..>4050
                     /locus_tag="VFE39_16300"
     CDS             2988..>4050
                     /locus_tag="VFE39_16300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HET6260199.1"
                     /translation="MSAPTRRLLRHVPAVRGFVGLAAAIGVVTALLVIAQAELLANTV
                     ARAFLGGAGPAELTVPLVALLGVVLGRAALGWGGEVASQRAAASAVAQLRAVVLDHVL
                     RLGPRHPGLPSSGQLATLAGRGVDGLDGYVGRYLPQLIIGAVVPLVVGIRIFTADWVS
                     ALLVGVTVPLIPLFMVLIGLHTRARTGRQWRALAVLGHHFLDVVAGLDVLTAYGRARR
                     QGRQIAAMSEQYRVETMRGLRVAFLSSLALELLATLSVALVAVSVGLRLVGGQLDLAT
                     GLLVIVLAPEVYQPLRAVAARFHDSAEGAVAAESVFAVLDTSPHGRVPVTEVPAPDPS
                     AGPVRLDAVGVDGRSGPVLD"
BASE COUNT          531 a         1450 c         1468 g          601 t
ORIGIN      
        1 tacgtcgtcg acacgagctt cttcccgagc atcggtgccg tgaacccgtc cctgacggcg
       61 atggcgaacg cgctgcgcgt cggtgaccac gtgctcgaac gcctcggctg acgacgcctg
      121 gcgaccacat cggacggaca ggagtacaca catggctgcg gatcccgtgg gcgcgctggt
      181 catcttcggg gcgaccggcg acctggccaa gctggagacg ttccccgccc tcgtcgggtt
      241 ggtggacagg ggggtgctca ccgtccccgt catcggtgtg gccaggagcg ggtggaacct
      301 ggaccggttc cgcgagtacg cggcacagtc cctacgcctc aacggcatgg acccggaggc
      361 accgtcggcc cggacgatgc tcggcctgct ccgttacgtg gacggggacc tggacgacag
      421 cgccacctac gaggcgatgt cccgggagat gggcgactgc aggcgggccc tgtactacct
      481 ggaggtgccc ccggtcctgt tcggacgcat cgccgagggg atcgcggcgg cggggcgggc
      541 cgacggcgct cgggtgatgg tggagaagcc gttcggcacc gacctggcca gtgcgatcgc
      601 cctcgaccag acgatgcacg accacttccc agaggacgcg atctaccgcg tggaccactg
      661 gctgggcctc gacccgctca acgacgtcct cgtcgcccgg ttcgccaact cgatgttcga
      721 accgctgctg gaccgtgacc acgtggagag cattcagatc acgatggccg aggcgttcga
      781 cgtcgccgac cgcggtcggt tatacgacca caccggcgcg atccgcgacg tcgtgcagaa
      841 ccacatgctg caggtgctcg ccaccgtcat cgccgaccct ccggacggat ccgggatgga
      901 ctcgtggctg gacgccaagt cacgggtgat ctccgcgctc cgaccgctga cccccgctga
      961 cgccgtccgc ggtcagtacg acggctatcg cgacgtggag ggcgtcgatc cggtgtcgac
     1021 cgtggagacc tacgtcgcgg tccggctcgc gctcgactcg tggcgctggg cgggagtgcc
     1081 gatcctcatc cgcgccggga agaccatgcc cgtgaccgcg acggaggtga gcatccggtt
     1141 ccggcccgtt cccttcgacg tgttcggtat ctcccgcact cgcatgcaca acacgctgcg
     1201 gttccggatc tggcccaaca cggagatcgg gctcgccctc gcggggaaga agcccggcgc
     1261 cgggcgcgag ccgcagatgc aggacctcgt gttcgccgag gacagcgcgt cggacatgcg
     1321 accctacgac cgcctcatcg gcgccgcgct cgacgggaac ccggtgctgt tcgcccgcca
     1381 ggacaccgtg gaggccgcgt ggcgggtcgt cgacccggtg ctcggcgacg cggtccccgt
     1441 gcagggatac cagcgcggta cctggggacc caaggaggcc gacagcctgt tgcccaacgg
     1501 tgacgactgg cacgacccgg tggcctgacc atcccggccg aggcatcgca gggcatcgga
     1561 ggaccgcgtg tcgccagatg atccggatcg gaccggacaa caccgtgtcg tgatcgtcgg
     1621 gggcggcttc ggtgggctct tcgccgcgcg ggcgctgcgt caccgggatg tcgccatcac
     1681 actcgtcgac cgcgcccagc atcacctctt ccagccgctc ctctaccagg tcgccaccgg
     1741 gatcctgtcc gaggggcaga tcgccgcgcc gctgcgtgat gtcctcaagc ggcaccgcaa
     1801 cgtcgaatgc gtgctcgccg acgtcgtgga cgtggacgcc gccggccgcc gggtcgtggc
     1861 cgtgcgcccc ggcggtgacc ggatgacgct gccctacgac gacctggtcg tggccgccgg
     1921 agtgcgccag tcctactacg ggcacgacga gttcgcgcga tgggcccccg ggatgaagac
     1981 cgtcgcggat gcgctggcga tccgccgcag ggtgttcggt gcgttcgagc tcgccgagac
     2041 cgcgacggac cctgacgagc agcggcgctg gctcaccttt gccgtcgtgg gtgccgggcc
     2101 gacgggagtg gagctggccg ggcagatccg cgaggtcgcc acccgcaccc tgcggaagga
     2161 gttccggcac atccgcccgg aggacgcccg agtcctgctg ttcgacgggg gcagcgcacc
     2221 gctcgcgtcc ttcgggaccg cgctgtccgc gaaggcggcc gcggcactgc gtgacctcgg
     2281 ggtcgagctg cacttcggct cgatcgtcac cgacgtggac ggagagggcc tgcaggtccg
     2341 cgaccgcgcc ggacagagca tctgccacgg ggcgggaacc gtgatctggg cggcgggggt
     2401 ggaggcgcca ccggtggccg aggcactcgc cgtcgccaca agcgcccggc gggatcgcgc
     2461 agggcggatc tgtgtggagc cggacctcac catccgcggc cacccggaga tctccgtcgt
     2521 cggggacatg atgagcctcg acgcgctgcc cggcgtggcc gaggtcgcga tgcagtcggg
     2581 gctctacgca gggcaccgga tcaggcagcg gctccgagac cggaccggca acggcaacgg
     2641 ccacggcacc ggcaagggca tcgcagcctt ccgctactac gacctcggct cggccgccta
     2701 cctgtcccgc ggcaacgccg tcgtctcggc gggcccggtg cggttcgccg gtttcggggg
     2761 ctggttggcc tggctgttca tccacctcgc cttcctcacc ggctaccgca accgcctcgg
     2821 cgcggtgatc acctgggccg ccgccttcat tcgggacgca cgacgcgagc gcgcattcac
     2881 cacgcagcag atcgagcggc tgcaggacgt ctacgagggg cctccccgga aggatcggcc
     2941 ggccgtcggg ccgtcaccgc cgcacccccc atgatcaccc gagcaccgtg agcgcgccga
     3001 cccggcgcct gctgcgccac gtcccggccg tccgcgggtt cgtcgggctc gccgccgcga
     3061 tcggcgtggt cacggcgctg ctggtgatcg cccaggccga gctgctggcg aacacggtcg
     3121 cccgggcgtt cctcggtggg gccgggccgg ccgagctcac ggtgccgctc gtcgccctgc
     3181 tcggcgtcgt actgggcagg gcggcgctgg gctggggcgg ggaggtcgcc tcccagcggg
     3241 cagcggcctc cgcggtcgcg cagctgcggg ccgtcgtgct cgaccacgtc ttgcggctcg
     3301 ggccgcgcca ccccggactc ccctccagcg ggcagctggc gacgctcgcg ggccgcggcg
     3361 tggacggcct cgacgggtac gtcggccgct acctccccca gctgatcatc ggcgccgtgg
     3421 tgccgctcgt cgtcggcatc cggatattca ctgccgactg ggtctctgcg ctgctcgtcg
     3481 gcgtcaccgt cccgctgatc ccgctgttca tggtgctgat cgggctgcac acccgggccc
     3541 ggacgggccg gcagtggcgg gcgctcgcgg tgctgggcca ccacttcctc gacgtcgtgg
     3601 ccgggctcga cgtgctgacc gcgtacgggc gagcgcgccg gcaggggcgc cagatcgcgg
     3661 cgatgtccga gcagtaccgc gtcgagacga tgcgcggcct gcgggtggcg ttcctgtcct
     3721 cactcgcact ggagctgctc gccacgctct cggtggcact tgtcgcggtg tcggtggggc
     3781 tgcggctcgt cggcgggcag ctggacctgg cgaccggcct gctggtgatc gtccttgccc
     3841 ccgaggtgta ccaaccgctg cgggccgtgg ccgcccggtt ccacgacagc gccgaggggg
     3901 cggtcgctgc ggagagcgtc ttcgcggtgc tggacacgtc gccccacgga cgggtgccgg
     3961 tcactgaggt gcctgcaccc gacccctcgg ccgggcctgt gcggctcgac gcggtcgggg
     4021 tggacgggcg gtccggaccg gtgctcgacg
//
LOCUS       DASRSC010000335         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5546002,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000335 DASRSC010000000
VERSION     DASRSC010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5546002"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VFE39_16305"
     CDS             <1..963
                     /locus_tag="VFE39_16305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET6260200.1"
                     /translation="ASVISVVIPNWNGRRWLPGCLAALAAQERAPDEIIVVDNGSSDG
                     SVEYLRHEHPEVTVLALSRNTGFAAAANIGVSIPAGEFVALVNADVELAPDWLARMAA
                     ALEAHPGAASVACKMLSLRDPTIVDDAGDVLRRDGVCEQRGRFGPDDGRWDAAGEVFG
                     ACAGAALYRRSAVVSAFGFHEPYFAYLEDVDLALRLRLAGWTCRYEPAVALHAAQGSS
                     GALPGGAAFLATRNTLVLVARFWPVRWLGLVAYRQLGWAWHAARERRLRAHLRAVAAA
                     VPLLPAALRARRALRHGAVIPICTAIPSRPWRGPSAEGRPGEHG"
     gene            complement(1183..>2316)
                     /locus_tag="VFE39_16310"
     CDS             complement(1183..>2316)
                     /locus_tag="VFE39_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET6260201.1"
                     /translation="ETVAWCRHDGTIERVKVSELYVTEALDRVEATEAAAGEIISVAG
                     IPEITIGDTLADADDPQPLPVITVDEPSLSVTIGINTGPLAGQDGTLLTASQVKARLE
                     RELVGNVSLRITETERPDVWEVQGRGELALAILVELMRREGFELTVGKPQVVTREIDG
                     KTNEPVERMSVDAPDEYVGVITQLMALRKGRMEQMVNHGTGWVRMEYLVPARGLIGFR
                     TEFLTETRGTGLVHHVFDRWEPWHGELRTRPTGSLVADRRGTVAAFALFNLQERGILF
                     VEPGDEVYEGMIVGENSRAEDLDVNAVKEKHLTNIRSSTADELVRLVPKRELSLDQAL
                     EFLRADECVEVTPHSVRLRKVTLDKTDRVREARAVRAAAGSAA"
BASE COUNT          295 a          845 c          816 g          360 t
ORIGIN      
        1 gcctcggtga tctccgtcgt catccccaac tggaacgggc ggcggtggct gcccgggtgc
       61 ctggcggccc tcgccgccca ggagcgcgcc ccggacgaga tcatcgtggt cgacaacgga
      121 tcgagcgacg gctcggtcga gtacctgcgc cacgagcacc ctgaagtgac ggtcctcgcc
      181 ctgtcgcgca acaccggctt cgccgccgcc gcgaacatcg gggtcagcat ccccgccggc
      241 gagttcgtgg cgctggtcaa cgcggacgtc gagctggccc ccgactggct ggcgcggatg
      301 gccgccgcgt tggaggccca cccgggcgcg gcgtcggtgg cctgcaagat gctgtccctc
      361 cgcgacccca cgatcgtcga cgacgccggc gacgtgctgc gccgcgacgg cgtctgcgag
      421 cagcgcggcc gcttcggtcc cgacgacggt cgctgggacg ctgcgggcga ggtgttcgga
      481 gcgtgcgccg gggccgcgct gtaccgccgg tccgcggtcg tgagcgcctt cggcttccac
      541 gagccctact tcgcctacct ggaggacgtc gacctcgccc tgcgcctgcg cctggccggg
      601 tggacctgcc gctacgagcc tgcggtcgca ctgcacgccg cgcagggctc ctcgggcgcg
      661 ctgcccggcg gtgctgcgtt cctggccacc cggaacacgc tcgtgctagt ggcccgcttc
      721 tggcctgtac gctggctggg actggtcgcc taccggcagc tcgggtgggc gtggcatgcg
      781 gcgcgcgaac ggaggctgcg ggctcatctg cgcgctgtgg ccgcggcggt tccgctgctc
      841 ccggcggcgc tgcgtgcgcg ccgcgccctt cgccatggcg ctgtcatccc gatctgcacc
      901 gcaattccgt cgcggccctg gcgcggcccg tcagccgagg gccgtcccgg ggagcacggg
      961 tagctgcggg ccctccgcgt ctgcgcgcga cacgaacgca cgtcaacggc gtctatcgag
     1021 gagagtgggt gcgggggcgg cctaccaaca accaatccgt tccaaacctg ggatcacggc
     1081 gccagtttgt ccgctggagc gcgcatagcg accacctcgt gccaaacgtg cggcaccgat
     1141 cccggtttgt caccgattgg cggcggaacc cgcgctcgaa gctcaggccg ccgagccggc
     1201 cgcggcccgc accgcccgcg cctcgcgcac ccggtcggtc ttgtcgagcg tcaccttgcg
     1261 caggcgcacc gaatgggggg tcacctcgac gcattcgtcg gcgcgcagga actcgagcgc
     1321 ctggtccagt gacagttccc gcttgggcac caaccgcacg agctcatccg ccgtcgagga
     1381 gcggatgttg gtgaggtgct tctccttgac cgcgttgacg tccaggtcct cggcgcgcga
     1441 gttctcgccg acgatcatcc cctcgtagac ctcgtcgccg ggctcgacga acaggatccc
     1501 gcgctcctgg aggttgaaca gcgcgaacgc ggccaccgtg ccgcgacggt cggccacgag
     1561 cgaaccggtg ggccgggtgc gcagctcgcc atgccacggc tcccagcggt cgaacacgtg
     1621 gtgcaccagg ccggtgcccc gggtctcggt gaggaactcg gtccggaagc cgatcagccc
     1681 ccgggccggc acgaggtact ccatgcgcac ccagccggtc ccgtggttga ccatctgctc
     1741 catgcgcccc ttgcgcaggg ccatcagctg ggtgatgacg ccgacatact cgtcgggagc
     1801 gtcgacgctc atgcgctcga ccggctcgtt ggtcttgccg tcgatctcgc gggtcaccac
     1861 ctggggcttg ccgacggtga gctcgaagcc ctcccggcgc atcagctcca cgaggatggc
     1921 cagggccagc tcgccgcggc cctggacctc ccatacgtcg gggcgctcgg tctccgtgat
     1981 gcggagcgag acgttgccga caagctcgcg ctccagccgg gccttgacct ggctggcggt
     2041 gagcagggtg ccgtcctggc cggccagcgg accggtgttg atgccgatgg tgaccgacag
     2101 cgacggctcg tccaccgtga tcaccggcag cggctgggga tcgtcagcgt cggccagggt
     2161 gtcgccgatc gtgatctccg ggatcccggc cacggagatg atctcgcccg ccgcggcctc
     2221 ggtggcctcg acgcggtcca gggcctcggt gacgtagagc tcggagacct tgacgcgctc
     2281 gatcgtgccg tcgtgcctgc accaggcgac ggtctc
//
LOCUS       DASRSC010000336         3716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5216275,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000336 DASRSC010000000
VERSION     DASRSC010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5216275"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..658
                     /locus_tag="VFE39_16315"
     CDS             8..658
                     /locus_tag="VFE39_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET6260202.1"
                     /translation="MVPAEEMGEYTDEPVPDRYSDGRRLDRSPREYGDDRWDDRFSYD
                     DDSQHDRYEDGWDRRPPRPVAMDRPVPRGMGGASGLGQPAVRGSLAIDPDAAMREPIR
                     AVPPAPPAVPEQRDRAARGAAALARITTLQPSSYKEARTIGERYRDGVPVIMNLTELD
                     VDDAKRLVDFAAGLAFALRGSIDKVTSRVFLLTPADVEVSADDARRLAERNIFRQD"
     gene            663..953
                     /locus_tag="VFE39_16320"
     CDS             663..953
                     /locus_tag="VFE39_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET6260203.1"
                     /translation="MPLAIQFLLYYVLFFFWLLLIGRIVAELVRSFARQWVPAGPSAV
                     ALEIIFTATDPPVKLLRRVIPVVRIGGVGLDLSIMVLLLVVFILMNVAQPTP"
     gene            1004..1873
                     /locus_tag="VFE39_16325"
     CDS             1004..1873
                     /locus_tag="VFE39_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HET6260204.1"
                     /translation="MPLTPADVHNVAFSKPPIGKRGYNEDEVDAFLDLVEAELARLIE
                     ENEDLREQVSQLEQRLGNAQADLEDARSRPPAQLGAPVSPPTQQIQRQVEPPPMRQME
                     PPRSMSGPPSLVDSGEQGDHHVQVAKVLSMAQEMAERLTSEAKSEADAMLTDARTKSE
                     QLLSDARTKSDGLVNDARSRAETMLNDARTRAETLERQSRDKAATLVGDAERKQAEII
                     GTITRDKSVLEKKIDELRTFEREYRTRLKTYLESQLRDLGERGSAAPSDARSSSNGGY
                     ASTGYGQRADAGS"
     gene            1934..3193
                     /locus_tag="VFE39_16330"
     CDS             1934..3193
                     /locus_tag="VFE39_16330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260205.1"
                     /translation="MLLLVLILVLIAFGLLVVALLSGSVLWAWVSVVVSVAAAAVLLI
                     DWLQRRNAVKAGTDAAAGTGPDASRPRSEVEPVTEILPVIPPSGVPMAGAGGVNGAPR
                     DVPAARFDQVLDGTQTVIMPVVQPSGSSDRPPGATPGISPSGGDSSPSVTKTGGDRSS
                     SVPRPSDAGDVTGIASGSAEKPAIRPDDRPAASAAPSATSPEVAGTAPRVPGTTGVPS
                     GASAPVPVDLEKADRQQADRDATDRDTTPEPGAAPASDGPADRRDASAEATVAVDARQ
                     ADTAAGPAVPDGAPADGSAADAYVEASSATAAAVEDGSPAADATATAVGEATAGEPVL
                     PPPGSDVEPPEEPRDVAAAALIAALEDEVLVIDEQPRYHVESCRSLAGHALIPLPARE
                     AIELGFTPCGWCAPDRTLASRYPAPAR"
     gene            3466..>3716
                     /locus_tag="VFE39_16335"
     CDS             3466..>3716
                     /locus_tag="VFE39_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HET6260206.1"
                     /translation="MKAEATADHAGYPLVPAHPSFPSLEREVLDAWDADGTFVASVEA
                     RPAGENGSNEFVFYDGPPFANGLPHYGHLLTGYVKDVVPR"
BASE COUNT          563 a         1357 c         1273 g          523 t
ORIGIN      
        1 cttcggcatg gtgcccgccg aagagatggg cgagtacacc gacgagccgg ttcccgaccg
       61 ctactccgac ggacgccggc tcgaccggtc ccctcgcgag tacggcgacg accggtggga
      121 cgaccgcttc tcctacgacg acgactccca gcacgaccgg tacgaggacg ggtgggaccg
      181 ccgcccgcct cgtccggtgg ccatggaccg gcccgtgccg cgtggcatgg gaggtgcgtc
      241 ggggctgggt cagcccgcgg tccggggctc gctcgcgatc gaccccgacg ccgcgatgcg
      301 cgagccgata agggccgtcc cccctgctcc gcccgccgtt cccgagcagc gcgaccgcgc
      361 cgcacggggg gccgccgcgc tggccaggat caccaccctg cagcccagca gctacaagga
      421 ggcgcgcacg atcggcgagc gctaccgcga cggcgtgccg gtgatcatga acctcaccga
      481 gctcgacgtc gacgacgcga agcggctcgt cgacttcgcc gccgggctcg cgttcgcgct
      541 gcgcgggtcg atcgacaagg tcaccagccg tgtctttctt ctcacacctg ccgacgtcga
      601 ggtgtccgcc gacgacgccc gcaggctcgc agaacgaaac atcttccgcc aggattgagg
      661 gcgtgcccct cgccatccag ttccttctgt actacgtgct gttcttcttc tggctgctgc
      721 tcatcgggcg gatcgtggcg gagttggtac gcagcttcgc ccgccagtgg gtgcctgcgg
      781 ggccgagcgc cgtggcgctc gagatcatct tcacagccac cgatccgccc gtgaagttgt
      841 tgcggagagt gatcccggtc gtccggatcg gcggcgtcgg actggacctg tcgattatgg
      901 ttctactgct ggtcgtgttc atacttatga acgttgccca gccgacgccg tagtgagccc
      961 cagtcacacg gtgccgccgc cgaggactgc atgaggtgat ccgatgccgc tgacgcccgc
     1021 cgacgtccac aacgtcgcgt tcagcaagcc ccccattggt aaaagggggt acaacgagga
     1081 cgaggtcgac gcgttcctcg acctggtcga ggccgaactg gctcgcctga tcgaggagaa
     1141 cgaggacctg cgcgagcagg tctctcagct cgaacagcgc ctcgggaacg cacaggccga
     1201 tctggaggac gcgcgctcgc gtccgcctgc tcagttgggg gcgccagtgt cgccgccgac
     1261 gcagcagatc cagcggcagg ttgagccgcc gcccatgcgg cagatggagc caccgcgctc
     1321 catgtcgggc cctccgtcgc tggtagacag cggtgagcag ggcgaccacc acgtccaggt
     1381 ggcgaaggtc ctgagcatgg cccaggagat ggccgagcgg ctcacctccg aggccaaatc
     1441 cgaggccgac gcgatgctca ctgacgcccg taccaagtcc gagcagctgc tgtcggacgc
     1501 tcgtaccaag tccgacggtc tcgtcaacga cgcccgctcg cgggccgaga cgatgctgaa
     1561 cgacgcccgt accagggcgg agaccttgga gcggcagtcg cgcgacaagg cagccacact
     1621 ggtcggtgac gccgagcgca agcaggcgga gatcatcggc acgatcaccc gcgacaagtc
     1681 ggtgctcgag aagaagatcg acgagctgcg cacgttcgag cgtgagtacc gcacccggct
     1741 caagacgtac ctggagtccc agctcaggga cctcggagag cgcggttcgg ctgctccgtc
     1801 cgatgctcgg tcgagctcaa acggcggcta cgcctctacc ggatacggcc agcgcgccga
     1861 cgccggtagc tgaccgagac ctacacgggc ggggcggcgg gttcccgccg caccgccgca
     1921 cacggagaga atcgtgctgc tcctggtcct gatcctcgtc ctgatcgcct tcggcctgct
     1981 ggtggtggcg ctgctgtccg gcagcgtgct gtgggcctgg gtgtccgtcg tagtcagcgt
     2041 cgccgcagcc gcagtactgc tgatcgactg gctgcagcgc cgcaacgccg tgaaggcggg
     2101 caccgacgcg gcggccggca cgggccccga tgcctcacgg cccaggtcgg aggtggagcc
     2161 ggtcaccgag atcctgccgg tcatcccgcc gtccggagtc cccatggccg gggccggcgg
     2221 cgtcaacggc gcgccccggg acgtcccggc ggcccgcttc gatcaggtcc tcgacggcac
     2281 gcagacggtg atcatgccgg tcgttcaacc gtccggctct agcgatcgac cgcccggcgc
     2341 tacccctgga atcagcccat cgggcggtga ttcgtcaccg agcgtaacca aaaccggtgg
     2401 cgatcgttct tcctcggtcc cgcgcccgag tgacgcggga gacgtcaccg gcatcgcgtc
     2461 cggctccgcc gagaaaccgg cgatccggcc cgacgaccgg cccgctgctt ctgctgctcc
     2521 ttccgccacg tccccggagg tcgcgggcac cgcaccccgc gtgcccggca ccaccggtgt
     2581 cccctctggc gcgtccgccc ccgtaccggt cgacctcgag aaggccgatc gccagcaggc
     2641 cgatcgcgac gcgaccgatc gcgacacgac gccggagccg ggtgccgcac ccgcgtcgga
     2701 cggcccggcg gaccgacggg atgcgtccgc ggaggccacc gtggccgtcg atgcgcggca
     2761 ggcggacacg gccgcgggtc ctgctgtccc ggatggggcc cctgctgacg gctcggccgc
     2821 cgacgcgtac gtcgaggctt cctccgccac cgctgcggct gtggaggatg gctcgcctgc
     2881 ggctgacgcc accgccaccg ctgtgggcga ggccacggcg ggcgagccgg tcctgccccc
     2941 gcccggctcc gacgtcgagc cgccggagga gccgcgggac gtcgctgcgg ccgcgctgat
     3001 cgccgccctc gaggacgagg tactcgtgat cgacgagcag ccccgttacc acgtcgagag
     3061 ctgccggtcg ctggccggcc acgcgctgat cccgctgccg gcgcgcgagg ccatcgagct
     3121 cggcttcacc ccctgcggct ggtgcgcgcc cgaccgcacg ctggccagca ggtaccccgc
     3181 tccggcccgg taggccccac cggaataccg agtggcgaca gggcgctacg cttgatgcac
     3241 aggcaccgat ccggccatca ccggggagcc tccggaagaa caggcgtcca gcccggcaac
     3301 gggcagggcc cgcctcagta gaaccggacg ggagcggccc gtcacagccg accgttgagt
     3361 ggctgcccac cttgggtggc aagcggggtg gtaccgcggc gcccgcgagg gccgtcgtcc
     3421 ccgtgcaggc agcagcacca gccacgcacg aggagcacac gcgcaatgaa ggccgaggcc
     3481 acagccgacc atgccgggta cccccttgtg ccggcgcacc cgtcctttcc ctcgctggag
     3541 cgcgaggtgc tggacgcctg ggacgccgac ggcaccttcg ttgcgtcggt cgaggcgcgt
     3601 cccgctggtg agaacggcag caacgagttc gtcttctacg acgggccccc cttcgccaac
     3661 ggcctgccgc actacgggca cctgctcacg ggctacgtca aggacgtcgt gccgcg
//
LOCUS       DASRSC010000337        14502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1778629,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000337 DASRSC010000000
VERSION     DASRSC010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 14502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14502
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1778629"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VFE39_16340"
     CDS             complement(<1..476)
                     /locus_tag="VFE39_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020502217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET6260207.1"
                     /translation="MIGAGQLARMTHQAAIALGQSLRVLAEHPSDPAALVCADVRAGA
                     ATDLSALHHFARGCAVVTFDHEQVPQENLRALVGVGVTVHPHPDALLHAQDKLVMRRR
                     LAGLGAAAPQFAEVRHAGDVERFGAEHGWPVVLKAVRGGYDGRGVWMLDSPDPALVA"
     gene            573..1082
                     /locus_tag="VFE39_16345"
     CDS             573..1082
                     /locus_tag="VFE39_16345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET6260208.1"
                     /translation="MPLVESVLAAIPQPYREVAVKHRELVKFAVVGGTTWVIDTAIFL
                     FLKGTVLSDKPLTAKVISVLVATIVSYVMNREWSFRTRGGRDRHHEAALFFIISGVGV
                     AVYTAPLAISRYVLDLKVPGVSLLTQEVADFVSGQIIGVLVGMAFRWWAFRRFVFPDE
                     NARSKIPTG"
     gene            complement(1093..2379)
                     /locus_tag="VFE39_16350"
     CDS             complement(1093..2379)
                     /locus_tag="VFE39_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011875056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6260209.1"
                     /translation="MRRRILQSTLLVVAITALVLGGPLTITTWRLVEDFTRGELTSSL
                     ERVAGRLDAMAGPNGVDPAQIDMRALELAIPAGGRLVLQLPGQAPQVIGPEVGANPVL
                     ETIPFGPGGSITLEEPNLVLRAEQVQVALVVALLVVLSVATGAVVATVTARRLAEPLS
                     GVADRAARLGAGDFRSAPERHGIPELDRVSDVLDTSAAALAELVQRERALVGDVSHQL
                     RSRLTALQLRLDELAAHPDPSARREALAALEQAERLSGVLDELLEAARAARAAGAQLQ
                     DLREGLSTVADEWRPALRAAGRSLKVRVPAGLLARVTPTRIREAVGALVENAIQHGAG
                     TVTLSARATENSLLIEVSDAGSGVPEELVPHVFDRGVSVGSSTGLGLALARALVEADG
                     GRLELSRARPPMFTIFLPIARADDVMGAPRPVTTPR"
     gene            complement(2392..3048)
                     /locus_tag="VFE39_16355"
     CDS             complement(2392..3048)
                     /locus_tag="VFE39_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6260210.1"
                     /translation="MTTVLLAEDDAAIAEPLSRALQREGYEVDVAVDGEAALERVRCG
                     HVDLLVLDLGLPGMDGLEVCRRVRLDAPDLPVLMLTARTDEVDFVVGLDAGADDYVGK
                     PFRLAELLARVRALLRRLTPESVEVGGVRMELSGRRVLVDGAEVGLANKEFELLRVLL
                     LRAGQVVTRDEILREVWNDPDMKTSKTLDMHMSWLRRKIGGERRIATVRGVGFRFNHD
                     "
     gene            3247..4026
                     /gene="hisN"
                     /locus_tag="VFE39_16360"
     CDS             3247..4026
                     /gene="hisN"
                     /locus_tag="VFE39_16360"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020633580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HET6260211.1"
                     /translation="MDSDLELALRLADTADAITLDRFRAADLRVTRKPDRTPVTDADT
                     AAEDAIRRTISAERPDDAVLGEERGGDVPESGRGWVLDPIDGTKNFSRGMPVWATLIA
                     LTVDGEPVVGVASAPALGRRWWAARGAGAWTSDTPGGEPRRIGVSGVTDLADAYVSTT
                     DIQTFAGLGRREHYLRLVDACWESRAFGDFWQHCLVAEGVLDIAVDVLASTWDLAALM
                     PILTEAGGALTDLGGVSTFAGGDGLSSNGEVHDAALAIIGR"
     gene            4037..4486
                     /locus_tag="VFE39_16365"
     CDS             4037..4486
                     /locus_tag="VFE39_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET6260212.1"
                     /translation="MIELTYEDFTPGRCFDLGTTVVDGDEMVAFARRFDPQPFHVDDD
                     AAAASIFGGLAASGWFTAGLWMRAYVDSVLARAASVGSPGGEEIRWPAPVFAGDELRA
                     SMEVLEARRSRSRPGLGLVRLRAFMHRGDELVYRSTFTGMFATRDAV"
     gene            complement(4508..5677)
                     /locus_tag="VFE39_16370"
     CDS             complement(4508..5677)
                     /locus_tag="VFE39_16370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6260213.1"
                     /translation="MTRMRFGIFLAPFHRAGENPTSALQRDLKLIEHLDELGYDEAWI
                     GEHHSAGSEIIASPEIFIAAAAERTRNIRLGTGVTSLSYHNPLWVAERMVLLDHLTRG
                     RVMLGVGPGSLPTDSAMIGLNPTDTRELLEENLDVVVRLLRGDEPVNAKTRTHTLINA
                     RLHLRPYSDPLFDVAVAAVASPTGPRLAGRHGLGLLSIGATLTKEGFDALAHHWNVME
                     ERATTYGTVVDRLKWRLVGLMHIAETREQAYKDVEYGIEQWFRYFQKVAAFLQMAVEG
                     GEIREMIDYVNEAGIGAIGTPDDATAQVQKLVAQSGGFGAMLLLAHEWANPEATRRSY
                     ELIAQHVMPQFQGQAQATLDAKARATETRSGHAEQQNAAVAHMTEKYEKELADRG"
     gene            complement(5754..6671)
                     /locus_tag="VFE39_16375"
     CDS             complement(5754..6671)
                     /locus_tag="VFE39_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HET6260214.1"
                     /translation="MVVPDSELPERVTIYEVGARDGLQNESAVVPVEVKAEFLDRLAA
                     AGLRVLEATSFVHPRWVPQLADAADLLQRLVRVDGVDYPVLVPNERGLDRALAAGVQH
                     VAIFASATETFAKRNLNRSLDEQFAMFAPVVDRALAQGLRVRGYVSMCFGDPWEGPVA
                     PEQVAAVGRRLVEMGCHQLSLGDTIGTGTPGHVDAVLDACAAGGVPMQQVAVHFHDTY
                     GQALANTLAALRRGVTTVDASAGGLGGCPYAESATGNLATEDLVWMLDGLGIQHGVDL
                     HALVATSTWMARHLGRPSPSNVVRALSGT"
     gene            complement(7014..7502)
                     /locus_tag="VFE39_16380"
     CDS             complement(7014..7502)
                     /locus_tag="VFE39_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007031913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HET6260215.1"
                     /translation="MAYPDDLLVEGEQVVMHKHPHWKMLVVPVLVLLLVVGVGTFLAA
                     LVRTTTWAPWAWLALAVVGLGLVVRLTVVPVVRWRTTHFVVTNRRVLVREGVLTRHGI
                     DIPMRRINSVQFRHTVFERILGCGTLVIESASDEPLEFDDVPGVQKVHTMLYDELADA
                     QT"
     gene            complement(7519..8325)
                     /locus_tag="VFE39_16385"
     CDS             complement(7519..8325)
                     /locus_tag="VFE39_16385"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HET6260216.1"
                     /translation="MNRIAQPLDADVLRTSLVGPWAQVDVVERTGSTNADLMAAAAAG
                     APDRTVLVAEHQEAGRGRLARSWVAPPGSGITVSVLFRPQGVPPSRFGWLPLLAGLAT
                     LDAVRELTAAPAGLKWPNDLLLGAGSRKVAGILAEVADPGRPAVVVGIGLNVVASPPD
                     QPGATSLAAEGGPSDRSAVLVALLTRLLEREASWREGRGDADATRLRADYRAGCASLG
                     SEVRVELPGGTVLTGIAEDVDRDGRLLLLDAAGHRRAVAAGDVVHLRPAD"
     gene            8568..10211
                     /locus_tag="VFE39_16390"
     CDS             8568..10211
                     /locus_tag="VFE39_16390"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HET6260217.1"
                     /translation="MSAATEPIADASGAAAGEPDIHTTAGKLADLYQRIDGAVHAGSE
                     AAVERQHAKGRQTARERIDQLLDPGSFVELDALTRHRSTNFGMQEKRPFGDGVVTGTG
                     TIDGRPVAVFSQDATVFGGALGEVHGEKIVKVMDLATKIGCPVIGINDGGGARIQEGV
                     VALGLYAEIFVRNVRSSGVVPQISLIMGPAAGGAVYSPVLTDFTVMVDGTSHMFITGP
                     DIIKTVTGEEVDFEDLGGGRAHNTKSGVAHYLADDEADAFAYVKELLGFLPSNNLSEP
                     PSYPAPEPTAGAISDGLTETDLELDSLVPDSPNTPYDIREVINRVVDDGEFLEVHELF
                     APNIVVGYGRVEGRSVGVVANQPTQFAGCLDIDASEKAARFVRTCDAFNVPVLTFVDV
                     PGFLPGTEQEWNGIIRRGAKLIYAYAEATVPKVTVITRKAYGGAYDVMGSKHLGADVN
                     VAWPTAQIAVTGSAGAVSILYRKELTGLEGDELAARRAELQQEYEDTLANPYIAADRG
                     YIDGVIPPSHTRGYVGRALRMLATKREAVPTRKHGNIPL"
     gene            10208..10459
                     /locus_tag="VFE39_16395"
     CDS             10208..10459
                     /locus_tag="VFE39_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit epsilon"
                     /protein_id="HET6260218.1"
                     /translation="MTPLGRPGEEPTPEERRALFRVVRGEPTDEEVAALTVVLAAAAA
                     SAGEPAVPSVRDRWSDPTARFRVPLHPGPGAWTRSTWPR"
     gene            10614..10886
                     /locus_tag="VFE39_16400"
     CDS             10614..10886
                     /locus_tag="VFE39_16400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260219.1"
                     /translation="MDAMIIRIGLVALVGLAVLIALASIPSRGKRERNAGRATRSHPY
                     VVRHGSRPGDGGYHGAFSVGHDSGGGCGGGSHGGSGDGGSGGGGGC"
     gene            10995..11621
                     /locus_tag="VFE39_16405"
     CDS             10995..11621
                     /locus_tag="VFE39_16405"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HET6260220.1"
                     /translation="MRVVLASASPARLSVLRAAGLDPLVEVADVDEEALLEALPGAPP
                     AEKVTHLAGAKAATVARRVGAAHPDAVVIGCDSMLLVDGELVGKPSGVEQARKRWRTM
                     AGATGELFTGHAVLRLADGEIERVAEGHATTTVRFADPSDAELEAYLATGEPLAVAGA
                     FTLDGRGGWFVDGIDGDPSNVIGISLPLTRRLLAGVGVGVTELWTPTR"
     gene            11799..12704
                     /locus_tag="VFE39_16410"
     CDS             11799..12704
                     /locus_tag="VFE39_16410"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HET6260221.1"
                     /translation="MALPNNTDPKLAEYAHPERLVTTEWLAQNLGADGLVVVESDEDV
                     LLYDTGHIPGAVKVDWHTELNDPVTRDYVDGQGFAELCGERGISRDTTVVFYGDRNNW
                     WATYALWVFSLFGHPDVRILDGGRAKWVAEGREMTTDQTKPSPVEYPVVERDDATIRA
                     FKDEVLAHLGLVGRGGALVDVRSPGEYSGELLHMPDYPQEGAVRGGHIPGAASVPWAR
                     AAAEDATFKPRSDLEAIYLQEQGLTADDDVVAYCRIGERSSHTWFVLTHLLGFGRVRN
                     YDGSWTEWGNSVRVPIVQGSERGSA"
     gene            12790..13221
                     /locus_tag="VFE39_16415"
     CDS             12790..13221
                     /locus_tag="VFE39_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HET6260222.1"
                     /translation="MSLPPQLAELVEDFSDVGPKDRLQLLLELSQELPELPERYAHAA
                     DTMEQVHECQSPLFLAVEVAPDDAVHLFFSAPREAPTTRGFASIMHTGLDGEPAAEVL
                     AIPDDFYTALGLAQAVSPLRLRGMAAMLARIKNQVRSATAA"
     gene            13375..14289
                     /locus_tag="VFE39_16420"
     CDS             13375..14289
                     /locus_tag="VFE39_16420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04222 domain-containing membrane protein"
                     /protein_id="HET6260223.1"
                     /translation="MTLAATADTWGISGPAFLVAYLLIAAAVWVATFRARRSLADPQA
                     TSPVADVTARLHDVAYLNGGAELAVYSALSSMHLRGTITSARGAVQAAGRLDPGADDL
                     ERAIHFTARVAMQRNRLQFHRPVLVALAVIEKRLVTAGLLLSEEQRLRIRRLGSWMLA
                     VAGLGLVRLLAGVAEAKPVGYLLLALVMVTAVAAVQLARAPRRTRLGDRTLAALRSEH
                     HGLSPEVKPDWSVYGPAGAALGIGIFGMGALWASDPAFADELAAQRVAAGGSSGDGGG
                     GFSSYGGDGGGGGGGGGCGGGGGGGCGG"
     gene            14282..>14502
                     /locus_tag="VFE39_16425"
     CDS             14282..>14502
                     /locus_tag="VFE39_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020422374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HET6260224.1"
                     /translation="MAERHALLPAGVGVGWRPEIAGWVARLPGPAFCEVIAETLAPAA
                     PPRGVVELREQGIPVVPHGVRLSLGGAEPV"
BASE COUNT         2097 a         5187 c         5199 g         2019 t
ORIGIN      
        1 gcgaccagcg ctggatccgg agagtcgagc atccagacgc cgcgcccgtc gtacccgccg
       61 cggacggcct tgagcacgac gggccacccg tgctcggcgc cgaagcgctc gacgtcgccg
      121 gcgtggcgca cctccgcgaa ctgcggcgcg gcggcaccga gcccggccag cctccgccgc
      181 atcaccagct tgtcctgggc gtgcagcagc gcgtccgggt gcgggtggac ggtcaccccc
      241 acgccgacga gcgcgcgcag gttctcctgc ggcacctgct cgtggtcgaa ggtgaccacc
      301 gcgcatccgc gcgcgaagtg gtgcagcgcc gacaggtcgg tggccgcccc ggcacgcacg
      361 tcggcgcaga ccagcgcggc cgggtcggag ggatgctccg cgagcacgcg aagcgactgg
      421 ccgagggcga tggcggcctg gtgggtcatg cgggccagct gaccggcacc gatcatgccg
      481 acgacgggga ggggatgaga agaggacgcg tccacgatgc gggaagccta cctgcgggcg
      541 cctcccggcc ggcgtctggc acgatcgaac aggtgccctt ggtcgaatcg gtgctggccg
      601 ccattccgca gccctaccgg gaagtcgccg tcaagcaccg cgagctcgtc aagttcgccg
      661 tggtcggcgg cacgacgtgg gtgatcgaca ccgcgatatt cctgttcctc aagggcacgg
      721 tgctcagcga caagccgctc acggcgaaag tcatctccgt gctggtggcg acgatcgtgt
      781 cctacgtgat gaaccgcgag tggtccttcc ggacccgcgg cggccgtgac cgtcaccatg
      841 aggccgcgct gttcttcatc atcagtggtg tcggcgtggc ggtctacacg gccccgctgg
      901 cgatatcgcg gtacgtcctc gacctcaagg tgcccggcgt gagccttctc acgcaggagg
      961 ttgccgactt cgtcagcggt cagatcatcg gggtactggt cggcatggcg ttccgctggt
     1021 gggcgttccg ccggttcgtc ttccccgacg agaacgcgcg cagcaagatt ccgacgggct
     1081 gagcggcgcg gctcaccggg gcgtggtcac cgggcggggg gcccccatga catcgtcggc
     1141 ccgcgcgatc ggcaggaaga tcgtgaacat cggcggccgg gcccgggaca gctccagccg
     1201 tccgccgtcg gcctcgacga gcgcacgagc cagcgcgagc cccagaccgg tcgaggaccc
     1261 cacggaaacc ccgcggtcga acacgtgcgg caccagctcc tcgggcaccc ccgatcccgc
     1321 gtcgctgacc tcgatcagca ggctgttctc cgtcgcccgc gcgctgagcg tgaccgtgcc
     1381 ggcgccgtgc tggatcgcgt tctcgaccag cgccccgacc gcctcccgga tccgggtcgg
     1441 cgtgaccctg gccagcaacc cggccggcac gcgcaccttc agcgaccgcc cggcggcgcg
     1501 tagcgcgggg cgccactcgt cggccacggt gctcagtccc tcgcgaaggt cctgcaactg
     1561 tgcccctgcc gcccgagcgg cgcgcgcagc ctcgagcagc tcgtcgagca ctcccgagag
     1621 ccgctcggcc tgttccagcg cggcgagggc ctcccggcgg gcgctcgggt cggggtgcgc
     1681 ggccagctcg tcgagccgca gctgcagtgc cgtcaaccgg ctccgcagct ggtgcgacac
     1741 gtcaccgacc agcgcgcgtt cgcgctggac cagctccgcc agcgccgcag ccgaggtgtc
     1801 gaggacgtcg gagacgcggt ccagctccgg gatgccgtgc cgctccggcg cggagcggaa
     1861 gtcgccggcg ccgaggcggg cggcccggtc ggcgacgccg ctgagcggct cggccaggcg
     1921 acgggccgtc acggtggcca ccaccgcccc cgtggccacc gagagcacca ccagcagcgc
     1981 gacgacgagc gcgacctgca cctgctccgc gcgcaggacc aggttgggct cctcgagcgt
     2041 gatcgacccg ccgggtccga aggggatcgt ctccaacacc gggttcgcgc ccacctcagg
     2101 cccgatcacc tgcggcgcct ggccgggcag ctgcagaacg agccggccgc ctgccggtat
     2161 ggcgagctcg agcgctctca tgtcgatctg ggcggggtcg accccgttcg ggccggccat
     2221 ggcgtccagc cgcccagcta cccgctccag gctcgaggtg agctcaccgc gggtgaaatc
     2281 ctccacgagc cgccaggtgg tgatggtgag cggccccccg agcaccaggg cagtgatggc
     2341 gacgacgaga agtgtcgact gcaggatccg gcggcgcaaa cgacagaccg atcagtcgtg
     2401 gttgaaccgg aaaccgacgc cgcgaacggt cgcgatccgc cgctcaccgc cgatcttgcg
     2461 gcgcagccag gacatgtgca tgtcgagcgt cttggacgtc ttcatgtctg ggtcgttcca
     2521 gacttcccgc aggatctcat cgcgggtcac gacttggccg gcacgcagca gcagcacgcg
     2581 cagcagctcg aactccttgt tcgcgaggcc gacctcggcg ccgtcgacca gaacccgccg
     2641 gcccgacagc tccatgcgga ccccgccgac ctccaccgat tcgggggtga gccgacgcag
     2701 cagcgcccgc accctggcga gcagctcggc aagccggaaa ggcttgccca cgtagtcgtc
     2761 ggcgcccgca tcgaggccga cgacgaagtc gacctcgtcg gtgcgcgcgg tgagcatcag
     2821 aacgggcagg tcgggggcat ccagccgcac ccggcggcac acctccaggc cgtccatccc
     2881 gggcagaccg agatcgagga ccagcaggtc gacgtgcccg caccgcactc gttccagtgc
     2941 ggcctcgccg tcgacggcga cgtccacctc gtaaccctcg cgctgcagtg cgcgcgacaa
     3001 gggctctgcg atagcggcgt cgtcctcggc taacaggacg gtggtcacac ttgcagtcta
     3061 ggcgcctctt gaacggccaa cccacaatgc aggtgtgacc gaggaccctc gcacgtccgc
     3121 gccacccatg tccgacgaac tccgtatcat gcggggccgg ctcgaccggc tcgaccggca
     3181 caaccgtcac gccgctgcgg gagcggatcg agggcgtccc gggcgagtga ggcaggctct
     3241 cgtcacgtgg acagcgatct cgagctcgcc ctgcgtctcg ccgacaccgc ggacgcgatc
     3301 accctcgacc ggttccgcgc ggccgacctg cgggtgaccc gcaagcccga ccgcaccccc
     3361 gtcaccgacg cggacacagc ggccgaggac gcgatccgcc ggaccatctc cgccgagcgc
     3421 ccggacgacg cggttctcgg cgaggagcgc gggggggacg tgccggagtc cggccgcggc
     3481 tgggtcctgg acccgatcga cggtacgaag aacttctcgc gcggcatgcc cgtctgggcc
     3541 accctgatag ccctcaccgt ggacggcgag cccgtcgtcg gggtcgccag tgctcccgcg
     3601 ctgggcaggc ggtggtgggc cgcccgcggc gccggtgcct ggaccagcga caccccgggc
     3661 ggggaaccgc gacggatcgg cgtctccggc gtcaccgacc tggccgacgc ctacgtgtcc
     3721 accacggaca tccagacctt cgccgggctc gggcgccgcg agcactacct gcggctcgtc
     3781 gacgcgtgct gggagagccg tgccttcggt gacttctggc agcactgcct ggtggccgag
     3841 ggcgtgctcg acatcgccgt cgacgtgctc gcgagcacct gggacctggc cgcgctgatg
     3901 ccgatcctca ccgaggcggg tggcgccctg accgacctcg gcggcgtgtc gacgttcgcc
     3961 ggtggcgatg ggctctcgtc caacggcgag gtgcacgacg ccgcgctggc catcatcggg
     4021 cggtagggcg catccgatga tcgagctcac gtacgaggac ttcaccccgg gccggtgctt
     4081 cgacctgggc acaacggtcg tggacgggga cgagatggtg gcgttcgccc gtcggttcga
     4141 cccgcagccg ttccacgtcg acgacgacgc tgccgcggcc tcgattttcg gtggcttggc
     4201 ggcgtccggc tggttcaccg cggggttgtg gatgcgcgcc tacgtcgaca gcgtgctggc
     4261 ccgcgccgcg tccgtgggct ctcccggcgg cgaggagatc cggtggcctg cgcccgtctt
     4321 cgccggggac gagctgcgcg cgtccatgga ggtgctcgag gcacggcgct cccgcagccg
     4381 ccccggcctg gggctggtgc ggctgcgggc attcatgcac cgtggcgacg aactcgtcta
     4441 ccgcagcacg ttcacgggca tgttcgcgac ccgcgacgcg gtctagggcc gctgcagggc
     4501 gcgagactca gccccggtcg gcgagctcct tctcgtactt ctcggtcatg tgggcgacgg
     4561 cggcgttctg ctgctcggcg tgcccggagc gggtctcggt cgcacgcgcc ttggcgtcga
     4621 gcgtggcctg cgcctggccc tggaactgcg gcatgacgtg ctgggcgatg agctcgtagg
     4681 accgccgcgt cgcctctggg ttggcccact cgtgcgccag cagcagcatc gcgccgaaac
     4741 cacccgactg ggcgacgagc ttctgcacct gtgcggtggc gtcgtccggc gtgccgatcg
     4801 cgccgatgcc cgcctcgttg acgtagtcga tcatctcccg gatctcgccg ccctccaccg
     4861 ccatctgtag gaaggccgcc accttctgga agtagcggaa ccactgctcg atgccgtact
     4921 cgacgtcctt gtacgcctgc tcgcgggtct cggcgatgtg catgagaccg acgaggcgcc
     4981 acttcagccg gtctacgacg gtgccatacg tcgtcgcccg ctcctccatg acgttccagt
     5041 ggtgtgccag cgcgtcgaag ccctccttgg tcagggtggc gccgatggag agcaggccga
     5101 ggccgtgccg accggcgagg cggggcccgg tcggcgacgc gaccgccgcg accgcgacgt
     5161 cgaacaacgg gtcggagtac gggcgcaggt gcagccgggc attgatcagc gtgtgcgtgc
     5221 gcgtcttcgc gttcaccggc tcgtcgccgc gcagcagtcg aacaacgacg tcgaggttct
     5281 cctcgagcag ctcgcgcgtg tcggtcgggt tcaggccgat catcgcggag tccgtgggca
     5341 gtgagcccgg tccgaccccg agcatgacgc ggccgcgcgt gaggtggtcg agcagcacca
     5401 tccgctcggc cacccacaac gggttgtggt agctcagcga cgtcacgccg gtgcccagcc
     5461 tgatgttcct ggtgcgctcc gcggcggcgg cgatgaagat ctccggcgag gcgatgatct
     5521 ccgagccggc cgagtggtgc tcgccgatcc acgcctcgtc gtagccgagc tcgtcgagat
     5581 gctcgatcag cttcagatcg cgctgtaggg cgctggtcgg gttctcgcct gcgcggtgga
     5641 atggggcaag aaagatgccg aaacgcattc gggtcatgtg aactctccaa tcgttgcgga
     5701 cagtcaccac gaccgtaggc atgccaccct tccgggtgca cctcctcgtt cgatcaggta
     5761 ccgctgagtg cccgtacgac gttcgacggt gacggcctgc cgaggtggcg ggccatccag
     5821 gtgctcgtgg cgacgagcgc gtggaggtcc accccgtgct ggatacccag gccgtcgagc
     5881 atccagacga ggtcctctgt ggccaggttg ccggtggccg actctgcgta cgggcagccg
     5941 ccgagtccgc cggcgctggc gtcgacggtg gtgactccgc ggcgcagcgc ggcgagcgtg
     6001 ttggccagcg cctgtccgta ggtgtcgtgg aaatgcacgg cgacctgctg catcggcacc
     6061 ccacctgcgg cgcacgcgtc gagcacggca tcgacgtggc cgggtgtgcc ggtaccgatc
     6121 gtgtcgccca gcgagagctg gtgacagccc atctcgacga gcctgcgccc taccgcggcg
     6181 acctgttccg gcgcgaccgg gccctcccac ggatcgccga agcacatcga cacgtatccg
     6241 cgcacccgca gaccctgcgc cagcgcccgg tcgacgaccg gggcgaacat cgcgaactgc
     6301 tcgtcgaggc tgcggttgag attccgcttc gcgaaggtct ccgtggcgct ggcgaagatc
     6361 gccacgtgct gcacgccggc cgccagcgcg cggtccagcc cgcgctcgtt cggcacgagc
     6421 acggggtagt cgacgccgtc gacacgcacc agccgctgca gcaggtcggc ggcgtcggcg
     6481 agctggggca cccacctcgg gtgcacgaag ctggtggcct cgagcacccg gaggcccgcg
     6541 gccgccaggc ggtccaggaa ctcggccttc acctcgacgg gcaccaccgc cgactcgttc
     6601 tgcagcccgt cccgcgcgcc gacctcgtag atcgtgacgc gctcggggag ctcggaatcg
     6661 ggcaccacca tcgggaggtc agtggcgctt cgtccacgac cgcgcacccg ttcctcgcca
     6721 ccgttcgccg acatcacccc agcttgcggg caccgcggtc gccggtcaac gccactgctc
     6781 gaaacggatt gaaatgggcc ggagaagaca cgagaaccga tccgccgtcg ctagaattgc
     6841 ggcatgccgc aggaggccga gtgaccatta atccctttcg gccatcggat gcggaaagtc
     6901 ctgcaccatt gcctgatgct ggcgcgagcc gctgatgaac gaacctggcc gaccacggac
     6961 ttgacccggc ggtcgatcgc ggccaccacg tacgcgtggc tgtgaacgag ggactacgtc
     7021 tgggcgtcgg ccagctcgtc gtacagcatc gtgtgcacct tctgcacgcc cgggacgtcg
     7081 tcgaactcca gcggctcgtc cgacgccgac tcgatcacga gcgtgccgca gcccaggatc
     7141 cgctcgaaca ccgtgtgccg gaactgcacg ctgttgatcc ggcgcatcgg gatgtcgatg
     7201 ccgtggcgcg tgagcacccc ctcgcgcacc agcaccctgc ggttggtcac gacgaaatgc
     7261 gtggtgcgcc agcggacgac gggcacaacg gtgagccgga cgacgagccc gagcccgacc
     7321 acggcgagcg ccagccaggc ccacggcgcc caggtcgtgg tccggaccag cgcggcgagg
     7381 aaggtgccga ccccgaccac gagcagcagc accagcaccg ggacgacgag catcttccag
     7441 tgcgggtgct tgtgcatgac gacctgctcg ccctcgacga ggagatcgtc cgggtaggcc
     7501 acacgtccac ggtactgctc agtcggctgg tcgcaggtgc accacatcgc cggccgcgac
     7561 ggcgcgccgg tggcctgccg cgtcgaggag cagcagccgg ccgtcccggt cgacgtcctc
     7621 ggcaatgccg gtgagcaccg tgccgccggg gagctccacc cgcacctcgg accccaggct
     7681 tgcgcacccg gcgcggtagt ccgctcgcag ccgcgtggcg tccgcgtcac cgcggccctc
     7741 gcgccacgag gcctcgcgct cgaggagccg ggtgagcagc gcgacgagca cggccgagcg
     7801 gtcggaaggc ccaccctccg cggccagcga cgtggcgccg ggctggtcgg ggggcgacgc
     7861 gacgacgttc agcccgatgc cgacgacgac ggcggggcga ccggggtcgg cgacctccgc
     7921 gagaatcccg gccaccttgc gggacccagc accgagcagc aggtcgttcg gccacttcag
     7981 gcccgccggg gcagcggtga gctcgcggac tgcgtcgagt gtggcgagcc cggcgagcag
     8041 gggcagccag ccgaagcgcg acggcggcac gccctgcggc cggaacagga cgctgacggt
     8101 gatcccggaa ccgggcgggg ctacccagct gcgcgcaagc cggccgcgac cggcctcctg
     8161 gtgctcggcg acgagcaccg tgcggtccgg cgcgccggcc gccgcggcgg ccatcaggtc
     8221 ggcgttggtg gatccggtgc gctcgacgac gtcgacctga gcccagggcc cgacgagcga
     8281 cgtgcgcagc acatccgcgt ccagcggctg agcgatccgg ttcatagagg cggacgctac
     8341 cggcggtccg cgcccggtgc ggggtgcggc gcgatacccg cgacggatgg acatcgcggg
     8401 gcggcggacg tgccggtgga cgtcagcggc ggtgccgggc tgtgcggctc accacggcag
     8461 ctgccggtta ccaccgcatt aagctgcgca gccatgagca agcgcgcgag tggagcgtgg
     8521 gcacaacctg tgcccgagtt gctgaccgag cgcagtgagg gacgacgatg agcgcagcca
     8581 ccgagccgat cgccgacgcg tccggcgccg cagccggcga gccggacatc cacacgacgg
     8641 ccggcaagct ggccgacctc taccagcgca tcgacggcgc ggtgcacgcc ggcagcgagg
     8701 ccgcggtgga gcgccagcac gcgaagggcc gccagaccgc acgcgagcgg atcgaccagc
     8761 tgctcgaccc cggctcgttc gtcgagctcg acgccctcac ccggcaccgc tccaccaact
     8821 tcgggatgca ggagaagcgc ccgttcgggg acggtgtggt gaccggtacc ggcacgatcg
     8881 acggccgccc ggtcgcggtg ttcagccagg acgccacggt gttcggcggg gcgctcggcg
     8941 aggtgcacgg cgagaagatc gtcaaggtga tggacctcgc caccaagatc ggctgccccg
     9001 tcatcggcat caacgacggc ggtggcgcgc gcatccagga gggcgtggtc gcactcggcc
     9061 tctacgccga gatcttcgtg cgcaatgtgc gcagctcggg cgtcgtcccg cagatctcgt
     9121 tgatcatggg tccggccgcg ggtggcgccg tgtactcccc cgtactcacc gacttcaccg
     9181 tgatggtcga cggcaccagc cacatgttca tcacgggccc ggacatcatc aagaccgtca
     9241 ccggcgagga ggtcgacttc gaggacctcg gcggcgggag ggcgcacaac accaagtccg
     9301 gtgtcgcgca ctacctcgcc gacgacgaag ctgacgcgtt cgcctacgtc aaggaactgc
     9361 tgggcttcct gccgtccaac aacctctccg agccgccgtc ctacccggcg ccggaaccga
     9421 cggccggcgc gatctccgac gggctcaccg agaccgacct cgagctcgac tcgctcgtcc
     9481 cggactcgcc gaacaccccg tacgacatcc gggaggtgat caaccgggtc gtcgacgacg
     9541 gcgagttcct cgaggtgcac gagctgttcg ccccgaacat cgtcgtcggc tacggccggg
     9601 tcgagggaag gtcggtcggg gtggtcgcga accagcccac ccagttcgcc ggctgcctcg
     9661 acatcgacgc ctccgagaag gccgccaggt tcgtccgcac gtgcgacgcg ttcaacgtcc
     9721 cggtgctcac gttcgtcgac gtgcccgggt tcctgcccgg cacggagcag gagtggaacg
     9781 ggatcatccg ccgcggcgcg aagctgatct acgcctacgc cgaggccacg gtcccgaagg
     9841 tcacggtgat cacccgcaag gcgtacggcg gcgcgtacga cgtgatgggc tccaagcacc
     9901 tgggcgccga cgtcaacgtg gcctggccga ccgcgcagat tgcggtgacc ggttcggcag
     9961 gcgcggtcag catcctgtac cgcaaggagc tcacggggct ggagggcgac gagctcgctg
    10021 cccgccgtgc ggagctgcaa caggagtacg aggacacact cgccaatccc tacatcgcag
    10081 ccgaccgggg ctacatcgac ggcgtcatcc ccccgtcgca cacccgcggc tacgtcgggc
    10141 gcgcgctccg catgctggcc acgaagcggg aggccgtgcc cacccgcaag cacgggaaca
    10201 tcccgctgtg acgccgctcg gccgcccggg cgaggagccg acgcccgagg agcgcagggc
    10261 gctcttccgg gtggtgcggg gcgaacccac ggacgaggag gtggccgcgc tgaccgtggt
    10321 gctcgccgcg gcagccgcgt cggcaggtga gccggccgtg ccctccgtcc gcgaccgctg
    10381 gtccgacccc acagcgcgct tccgtgtccc cctccatccg ggccccggtg cgtggaccag
    10441 gtcgacctgg ccccgctgac gctgcgcggc cgcccagggg tggagcggcc gcctcaaggt
    10501 ctgctcaagt tcctggcctc ccggacgtga cccaccccac acttcaggtg atcgaccggg
    10561 ggtgaccgag tccacgggga cgccgggctc gcggccggga gggagggacc ggcatggacg
    10621 caatgatcat ccggatcggg ctcgtcgcgc tcgtggggct ggcagtgctg atcgccctgg
    10681 ccagcattcc gagccgcggc aagcgggagc gcaacgccgg gcgcgcgacc cgcagccacc
    10741 cctacgtcgt ccgtcacggc agtcgtcccg gggacggcgg gtaccacggt gcgttctccg
    10801 tcggccacga ctccggtggc ggctgtggcg gcggctccca cggcggcagc ggtgacggcg
    10861 ggagcggtgg gggcggtggc tgctgacgcc ggcccgccgc gccgggtccc cggcaccgag
    10921 ttgcatgacg gggcaccggc acgacctcgt ggcgggatga agccgccgcg ggaccggccg
    10981 tagggtcgga tgccgtgcgc gtcgtactgg cctctgcctc ccccgcccgc ctgtccgtgc
    11041 tgcgcgcggc cggcctcgac ccgctggtcg aggtcgcgga cgtcgacgag gaggcgctgc
    11101 tcgaagcgct gccgggcgcc ccgcccgccg agaaggtgac ccacctcgcc ggcgccaaag
    11161 ctgccaccgt ggcgcggcgc gtcggggccg ctcatcccga cgccgtggtg atcggctgcg
    11221 actcgatgct cctcgtcgac ggggagctcg tgggcaagcc gagcggcgtc gagcaggcgc
    11281 gtaagcggtg gcggacgatg gccggtgcca ccggtgagct gttcaccggc cacgccgtgc
    11341 tccgcctcgc cgacggcgag atcgagcggg tggccgaggg ccacgccacg acgaccgtac
    11401 ggttcgccga cccgagcgac gccgagctgg aggcgtatct cgccaccgga gagccccttg
    11461 cggtcgcggg tgccttcacc ctcgacgggc gcggcggctg gttcgtcgac ggcatcgacg
    11521 gcgacccgtc gaacgtcatc gggatcagcc tcccgctcac gcggcggctg cttgcagggg
    11581 tcggcgtcgg tgtgacggag ctctggacgc ctacacggtg acatgacctc cgacgaatcc
    11641 gggaaacgtc caggctcgcg cctccaccga cggtgcccag acgttcccga agcgacgggt
    11701 gagcgacagc agcagttgcc gaatgagcgt cgcccggcgt gaacctccgt ctcctgcgca
    11761 ggctgcgaac ggagaacggc gaggactagc cttggcgcat ggcgctgccc aacaacaccg
    11821 acccgaagct ggccgagtac gcccacccgg agcggctggt caccaccgag tggctcgcgc
    11881 agaacctggg tgccgacggg ctggtcgtcg tcgagtccga cgaggacgtg ctgctctacg
    11941 acaccggcca catcccgggc gctgtgaagg tcgactggca caccgagctg aacgaccccg
    12001 tcacccgcga ctacgtggac gggcaggggt tcgccgagct gtgcggcgag cgcggcatct
    12061 cccgcgacac caccgtggtc ttctacggcg accgcaacaa ttggtgggcc acctacgcgc
    12121 tctgggtgtt ctccctgttc ggccaccccg acgtgcgcat cctcgacggc ggccgggcca
    12181 agtgggtggc cgagggccgc gagatgacca cggaccagac gaagccgtcc ccggtcgagt
    12241 acccggtggt cgagcgcgac gacgccacga tccgcgcctt caaggacgag gtgctggccc
    12301 acctcgggct cgtgggccgg ggaggggcac tggtggacgt ccggtcgccc ggcgagtact
    12361 cgggcgagct cttgcacatg cccgactacc cgcaggaggg cgcggtccgc ggcggccaca
    12421 tcccgggcgc ggcgagcgtc ccgtgggccc gcgcggccgc tgaggacgcc acgttcaagc
    12481 cccgttccga cctggaggcc atatacctcc aggagcaggg cctcacggcc gacgacgacg
    12541 tggtcgctta ctgccgcatc ggggagcgct cgagccacac ctggttcgta ctcacgcacc
    12601 tgctgggctt cggcagggtc cgcaactacg acggcagctg gaccgagtgg ggcaactccg
    12661 tgcgcgtgcc gatcgtgcag ggatcggagc gcggctcggc atgagtgagc ttgcgcgcga
    12721 atcggtgtca cagcgcctgc gcgctgtggc cccatgctat gggccgaccg agcgcagcga
    12781 ggttgtgcga tgagccttcc gccgcagctc gccgagctgg tcgaggactt ctcggacgtc
    12841 gggccgaagg accggctgca gctgctcctc gagctctcgc aggagctgcc cgagctcccg
    12901 gagcgctacg cccacgcggc cgacacgatg gagcaggtgc acgagtgcca gtcgccgctg
    12961 ttcctggcgg tcgaggtcgc cccggacgac gcggttcacc tgttcttctc ggccccacgc
    13021 gaggcgccca ccaccagggg tttcgcctcg atcatgcata cgggcctgga tggggagccg
    13081 gccgccgagg tgctggccat acccgacgac ttctacacgg cgctcgggct cgcccaggcc
    13141 gtgagtccgc tgcggctgcg agggatggcg gcgatgctcg cgcggatcaa gaaccaggtg
    13201 cgttcggcca ccgcggcctg agcggtcgtc acagccaccg cggcctgagc ggtcatcacg
    13261 caccgacgac cgacgcacgc gggaacctca agtcctggtg aagaacgttc cctgcgctgc
    13321 gtgagggcgg caacactgtg aggcgtctca taaggacggc tcaggaggtg tgcgatgacc
    13381 ctcgcagcga cagctgacac ctggggcatc agcggtccgg cgttcctggt ggcctacctc
    13441 ctgatcgccg cggcggtctg ggtggccacg ttccgagccc gccggtcact ggcggatccg
    13501 caggccacga gcccggtcgc cgacgtgacg gcgcgtctgc acgacgtggc gtacctgaac
    13561 ggcggggccg agctcgccgt gtactcggca ctgagctcga tgcacctgcg cggcacgatc
    13621 acgtcggcgc gcggcgccgt ccaggccgcc ggcaggctcg atcccggagc cgacgacctg
    13681 gagcgcgcga tccacttcac cgcccgcgtc gcgatgcagc gcaaccggtt gcagttccac
    13741 cgcccggtgc tggtcgcgct ggccgtgatc gagaagcggc tcgtgaccgc gggcctgctg
    13801 ctgtcggagg agcaacggtt gcgaatccgg cggttggggt cgtggatgct cgcggtggcc
    13861 ggcctcggtc tggtgcggct gctggccgga gtggccgagg ccaagcccgt cgggtatctg
    13921 ctcctggcgc tggtcatggt gaccgcggtg gccgccgtgc agctcgcgag ggcgccccgg
    13981 cgcacccggc tcggcgaccg cacgctcgct gccctgcgct ccgagcacca cgggctgtcc
    14041 ccggaggtga agccggactg gtcggtctac gggcctgcgg gggctgccct gggcatcgga
    14101 atcttcggga tgggcgcgct gtgggcgtcc gacccggcgt tcgccgacga gctggccgcc
    14161 cagcgggtgg ctgcaggtgg gtcgtcgggc gacggcggcg gcgggttcag cagctacggc
    14221 ggcgacggcg gcggtggcgg tggcggtggc gggtgcggcg gaggcggtgg cggtggctgc
    14281 ggtggctgag cgccacgcgc tgctccccgc cggggtcggc gtcggctggc gaccggagat
    14341 cgccggctgg gtggcccgcc tgcccgggcc ggcgttctgt gaggtgatcg ccgagacgct
    14401 cgctcccgcg gctccacctc gaggggtggt ggagctgcgg gagcaaggca tcccggtggt
    14461 gccgcacgga gtccggctct ccctcggcgg agccgaaccc gt
//
LOCUS       DASRSC010000338        10995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5217158,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000338 DASRSC010000000
VERSION     DASRSC010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10995
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5217158"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFE39_16430"
     CDS             <1..208
                     /locus_tag="VFE39_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260225.1"
                     /translation="DLDRRLVAALQLNGRASWTPGYGDLYHYMTHVVGALPGVRTADM
                     TLQARTLKRAWVRINPDGTRERAT"
     gene            205..1122
                     /locus_tag="VFE39_16435"
     CDS             205..1122
                     /locus_tag="VFE39_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020495999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HET6260226.1"
                     /translation="MTTEQQPWQWDEPTWRGHVERVRAGTRLVPESWPRGARVAVAIS
                     FDPDHETIPLRDGETRPGKLAQGEYGARVAAPKILALLAEYGVPASFFMPAVSALLHP
                     DEVEAYAAGGHEVAVHGWIHERNMLLERSEELDLTARAIEVLEKLSGVRPVGIRTPSW
                     DFSDHTLAIIEQLGLRYDSSLMADDEPYELVAAGRPTGIVEIPVEWIRDDAPYLMMER
                     YGALRPYTPPRELLEIWRDEFDAAHAEGGLFQLTLHPHIIGHRSRLLVLRELLAHIAG
                     SAGVWFATHAEIAEVARAQLKPAPASAPS"
     gene            1119..1430
                     /locus_tag="VFE39_16440"
     CDS             1119..1430
                     /locus_tag="VFE39_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260227.1"
                     /translation="MSDLVTGPAPYARRDATLMDATADALGVWLGTQAKDRSGIWRDL
                     AVRHRPTEVPTHHRPVLGYAAHRGIAVPGLERLVTQIGELEAGAPMAEQRITDLSKAL
                     R"
     gene            1427..2626
                     /locus_tag="VFE39_16445"
     CDS             1427..2626
                     /locus_tag="VFE39_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020496000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HET6260228.1"
                     /translation="MTGPVPSPAVLDDVDVAAVHQWLADRSEDLVDDLVAYTERETPS
                     DDTALLAAGLSHVEGWLGDRLGRPRSKAHHESADHGDVLVLEYDGAGDVAGRGVLTVL
                     AHYDTVWGAGTLAEWPVRIDGDRLTGPGVFDMKAGLVQAVWAVRALRAAGLPHPPLRL
                     LLNGDEEIGSPFSRPLIEKASADAAAVLVFEASADGALKTARKGVGLFDVHVRGVESH
                     AGLEPAAGASAIDEIARVVRTLHAATDLDEGTTVNVGVLRGGTRSNVMAGAAEAEVDV
                     RVASTAAEARIDELLGSLRPHDPRAAITVHGGWNRPAMERGPAIAAMYALVRRAGERL
                     GVELAETSVGGASDGNFAAAMGRPVLDGLGAVGDGAHARHEYVSIAGMVERTAVAAGV
                     ITAFTHP"
     gene            2787..3725
                     /locus_tag="VFE39_16450"
     CDS             2787..3725
                     /locus_tag="VFE39_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HET6260229.1"
                     /translation="MPALARRRGAGPLALLVVVAAVLSGCSPGPGSGITVQAAQFPWS
                     AAKLTNAILAEVVAAHPELGVSTITTIQVGPATAWAGAQRGDVDLLSEVAMPNQEELA
                     AKAADRIHLVHETYGNAQQGWFVPTYATQPGQPLAGLRSVTQLNDYAVALGGKLIDAD
                     PSFITTKQNAARLAGYGLRLEQVTSSEAAQLAELRRAYERRQPILVYLYHPHWAFEVF
                     DLTQLEEPNPYQPDCFTTGNGACAMPAYAAWTAASTDLQRGAPRFHAMLERFELPLPD
                     VEAMLKAVDVDDADVDEVARRWVADHPAAVRSWASA"
     gene            3710..4948
                     /locus_tag="VFE39_16455"
     CDS             3710..4948
                     /locus_tag="VFE39_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010232448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HET6260230.1"
                     /translation="MGERMNAPARPMIRCKRVTKVFGPAAAGSGPAVDEALAMAHDGV
                     GRAEIQQRTDATLAVYDVSFDVLPGELFVVMGLSGSGKSTLVRLLNRLIEPSSGVIEV
                     DGRELGSLDDTELRELRNRRMSMVFQHFALLPHRTVRQNAEYGLKIRGVPDEERREKG
                     EWALEQVGLADRAEAHPGQLSGGMQQRVGLARALASDTDVLLMDEPYSALDPLIRRDM
                     QALLARLQSELRKTIVFITHDLNEAMLLGDRIMLLKDGRPVQVGTGPEILAAPADDYV
                     ADFVSDVDRTRVLTAADVMREARVVVRPDERPADVLRRLGESEATGAYVLDDDRRILG
                     VVRDDWLARAAATGTDSISRAGLDGDYLTTEPDRPLIDLVDQVGRNVVPLAVVDEGSR
                     LLGVVPRAAVLASLAAPGRS"
     gene            4951..5889
                     /locus_tag="VFE39_16460"
     CDS             4951..5889
                     /locus_tag="VFE39_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6260231.1"
                     /translation="MPRIEIGTYVEQFITWLLTAIPGVLDGISAVVTVVVDALTAVLA
                     GPPFVVWVLLLTVAALLVRGWAFAVFTLVAFLLIAGLNLWQETMETLAVVLVAAVVAT
                     AVGVPLGIWAARSRAVGTVLRPVLDLMQTTPVFVYLIPAVFFFGIGVVPGVVATTIFS
                     IPPAVRLTELGIRGVDPEVVEAAHAFGAHPRQILREVQLPMALPSIMAGVNQVIMLAL
                     SMVVVAGLAGAGGLGTVVVSAVTQLDIAAGVDGGLAVVILAIFLDRFTAALAERPNRG
                     MWGRLRSRRAGAATTAPRVAETRTRAEDPVPAPGLS"
     gene            complement(5997..7295)
                     /locus_tag="VFE39_16465"
     CDS             complement(5997..7295)
                     /locus_tag="VFE39_16465"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HET6260232.1"
                     /translation="MTARRDASPTSVRARRRRDRRRGPEQCRTGRGRPGGLRIADLAE
                     WAAGVRPTPGDEELARRALVDTVAGTLAAVDGPDTGWTAGSPAVLRWAAVGHVLDIDD
                     VHLPSSSRVSVVCVPATLACGGGSREYLAAAGVMARLGAALDREHDRAGWDATCTVGA
                     PAAAVAAGLALGLDATALATAMALAVPAASGVQGSCGAPDRPLRVGLAAAAGVRAAHL
                     AAAGAPVDPATLDQWFAMIGGVDALDGPLVPDGLTVKPYPCSDALQRVISAVRLLAPL
                     PRDQIAAIRVHIPQSALQPLIRSAPRTGQEATRSLEYAVAVALLDGFPGLASFTDAAV
                     GRPEAKQLAGLVDIRPEPGGIGILDGDVRITVELMDGTVEHAVLDVPDGHPRRPLSDA
                     DLSAKVAGCVGPELAATVLTLGWDDAAALLHGALPEGAAR"
     gene            7414..8220
                     /locus_tag="VFE39_16470"
     CDS             7414..8220
                     /locus_tag="VFE39_16470"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HET6260233.1"
                     /translation="MSAPPPDTGMRTVLTTLRVLEVVSELQPVGVSAVARATGIPKSS
                     VQRCLVTLGQAGWLTQAKEDTHHWVLTGRALSIGMRGSTEKGLREAALAVMQDLRDET
                     RETIHLLGFGEEHAPAGGVEPATDPALVVIDRLDGPEPVRTWVRLGTRVPLHATCAGL
                     AVLARLPGAEVDRLLRGRLERYSDHTIVDRDALAAELRDVRERGYAMADRSWRRGIGA
                     VAAAVIDAAGDPVAALVISTPMQRFDTDRARVLGPLVAAAASRVSNDLRF"
     gene            8315..8764
                     /locus_tag="VFE39_16475"
     CDS             8315..8764
                     /locus_tag="VFE39_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5709 domain-containing protein"
                     /protein_id="HET6260234.1"
                     /translation="MSSDDDRTDGYDNDSGLLEPEDSLDDRGVEDVLDEGYSPPERPL
                     GVDEWGTTPREEHDGEGLERRLARELPDGGTAEWDGLGDAGDTDGELIDDEVGTARSG
                     RLVASDNGGFSDEDDELYAEDVGVDGAAASAEEAAVHVVADRDDDET"
     gene            complement(8925..9203)
                     /locus_tag="VFE39_16480"
     CDS             complement(8925..9203)
                     /locus_tag="VFE39_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6158 family protein"
                     /protein_id="HET6260235.1"
                     /translation="MTDRSHGIAPQELDDDDLRREVAHLHETRHDTLLGGSESALRAH
                     TERMLALEREFLLRFPEDSAPDPMRTRAGSRESAGQSMPGRDLDDAPG"
     gene            9418..>10995
                     /locus_tag="VFE39_16485"
     CDS             9418..>10995
                     /locus_tag="VFE39_16485"
                     /inference="COORDINATES: protein motif:HMM:NF039462.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6461 domain-containing protein"
                     /protein_id="HET6260236.1"
                     /translation="MIGTGDEAGVPEAPAGLADALVEAVLSALPTVAARIPAAPVAAL
                     GRLAAPIAPADPLRPAPQRRSGVDHLLAERLLGALELTVPDVRLDVPDVLVQHVRAPL
                     PQPTGAVAGMAAGGESETRTAVVLLDQVAPGLRELVTGLVGALAEHERVAPLLEAAPG
                     AEPEVAAAHGGVHLALGVVTALAVLRAARLPIVGAEPPVVVGVALDATLRVLRQRPMP
                     AAYAAALRERLRDEYVVPRFSAGDVPVQEHRFAMVEGPFPPLAGEPVNGLVEAMPGGI
                     VVRTGVRGAAVPVWLRVLSEEPAVVDLATWHEVVDVSYTAVEGAASVLGPAAPAPAHL
                     LGQTPPWPGPVRARVSARGRDEHGDGREGGERYEIVVWPAPLAPEVVHKATDRLGHRL
                     RGEPEPPVVVRPEVAYRWVRGTGIGEAATVTVVTGSGAEEVLAAFGADPADPVPLAEL
                     FDRCGIDPWVAVLPVDGAVLALEYNGWQGAQGPVLRPASARGRAASMYWNVNALSSLS
                     FARGGEVLASFEPPPPTR"
BASE COUNT         1415 a         3940 c         4134 g         1506 t
ORIGIN      
        1 cgatctggat cggcggctcg tcgccgcgct ccagctcaac ggccgtgcgt catggacgcc
       61 cggctacggc gacctctacc actacatgac gcacgtggtc ggcgcgctgc ccggcgtccg
      121 taccgccgac atgacgctgc aggcccggac gctcaagcgg gcctgggtcc ggatcaaccc
      181 cgacggcacc agggagcgcg ccacatgacc accgaacagc aaccgtggca gtgggatgag
      241 cccacgtggc gcgggcacgt ggaacgcgtg cgggccggca cccggctggt gccggagagc
      301 tggccgcgcg gggccagggt ggcggtggcg atctccttcg accccgacca cgagacgatc
      361 ccgctccgcg acggcgagac ccggcccggg aagctcgcgc agggggagta cggcgcgcgc
      421 gtggccgccc cgaagatcct cgcgctgctc gccgagtacg gggtgcccgc cagcttcttc
      481 atgcctgcgg tgtccgcgct gctgcacccc gacgaggtcg aggcgtacgc cgccggtggt
      541 cacgaggtgg ccgtgcacgg ctggatccac gagcgcaaca tgctgctcga acgctccgag
      601 gagctcgacc tcacggcccg cgcgatcgag gtcctggaga agctcagcgg ggtgcgcccg
      661 gtgggtatcc gcaccccgag ctgggacttc tccgatcaca ccctggcgat catcgagcag
      721 ctcgggctgc gctacgactc gtcgctgatg gccgacgacg agccctacga gctggtggcc
      781 gcgggccggc cgacggggat cgtggagatc ccggtcgaat ggatccgcga cgacgccccg
      841 tacctgatga tggagcgcta cggcgcgctg cggccctaca ccccgccgcg cgagttgctg
      901 gagatctggc gcgacgagtt cgacgccgcc cacgccgagg gcgggctctt ccagctcacc
      961 ctgcatccgc acatcatcgg ccaccggtcc cggctgctcg tcctgcgcga actgctggcc
     1021 cacatcgcgg gcagcgcagg cgtttggttc gccacccacg cggagatcgc cgaggtcgct
     1081 cgcgcccagc tcaaaccggc gccggcgagc gcgccatcat gagcgacctg gtcaccggcc
     1141 cggccccgta cgcgcgccgc gacgccacgt tgatggacgc caccgccgat gcgctcgggg
     1201 tgtggttggg cacccaggcc aaggatcgca gcggcatctg gcgcgacctt gccgtgcgcc
     1261 accggcccac cgaggtaccg acgcatcatc gcccggtgct ggggtatgcc gcgcaccgcg
     1321 ggattgccgt ccctggtctg gaacggctgg tcacccagat cggcgagctg gaggcgggtg
     1381 ccccgatggc agagcagcgc atcaccgacc tgtcgaaagc gctccgatga ccggcccggt
     1441 cccgtccccg gctgtgctgg acgacgtcga cgtcgccgcg gtgcatcagt ggctcgccga
     1501 ccgcagcgag gatctcgtcg acgacctcgt cgcctacacc gagcgggaga cacccagcga
     1561 cgacaccgcg ctgctcgcgg ccgggctctc ccacgtggag ggctggctcg gcgatcggct
     1621 cggcaggccc cggagcaagg cacatcacga gtcggccgac cacggcgacg tgctcgtcct
     1681 cgagtacgac ggggccgggg acgtggccgg tcgaggagtg ctcactgtgc tggcgcacta
     1741 cgacacggtg tggggggcgg gcacgctggc cgagtggccg gtgcggatcg acggcgaccg
     1801 gctgaccggt cccggcgtgt tcgacatgaa ggccgggctc gtgcaggccg tctgggcggt
     1861 gcgggcgttg cgcgccgcgg gcctgcccca cccgccgctg cgcctgctgc tcaacggcga
     1921 cgaggagatc ggcagcccgt tctcccggcc actgatcgag aaggccagtg cggacgcagc
     1981 ggcggtgctg gtgttcgagg cgagcgcgga cggtgcgctg aagaccgcac gcaagggcgt
     2041 cggcctgttc gacgtgcacg tacgcggcgt cgagtcgcac gccgggctgg agccggccgc
     2101 cggggcgagc gcgatcgacg agatcgcccg ggtggtgcgc acgctgcacg cggccaccga
     2161 cctggacgag ggcaccaccg tcaacgtcgg tgtgctccgt ggcggcacgc gctcgaacgt
     2221 catggcgggc gctgcggagg ccgaggtcga cgtgcgcgtc gcgtcgacgg ctgccgaggc
     2281 ccggatcgac gagctgctgg gctcgctccg gccgcacgac ccacgcgccg cgatcaccgt
     2341 gcacggcggg tggaaccggc cggcgatgga gcgcggccca gcgatcgcgg cgatgtacgc
     2401 gctggtgcgg cgggccgggg agcgtctggg tgtcgagctg gccgagacgt cggtaggcgg
     2461 ggccagcgac ggcaacttcg ccgccgccat gggccggccg gtgctcgacg ggctcggtgc
     2521 cgtcggagac ggtgcgcatg cgcggcacga gtacgtgagc atcgccggca tggtcgagcg
     2581 caccgcggtg gccgccgggg tgatcacggc gttcacgcac ccgtagcggc cggttccgga
     2641 accgcccacc gggggccgga ggccgaccag gctgacggcg gggcgccggg taggcccgcg
     2701 gatcacgtgc gggtgatgct tcgagtggcg caccgagttc cgcccgggca gcatcgcgag
     2761 gtcgttgccc cggagaagga gccgccgtgc ccgctctcgc gcgccgtcgc ggcgccggtc
     2821 cgttggcgct gctggtcgtc gtcgcagcgg tgctcagcgg gtgcagcccc ggtcccggtt
     2881 cggggatcac cgtgcaagcc gcccagttcc cgtggagcgc ggcgaagctg acgaacgcga
     2941 tcctcgccga agtcgtcgcc gcgcacccgg agctgggcgt ctcgaccatc accaccatcc
     3001 aggtcggtcc ggcgacggcg tgggccggcg cccagcgggg ggatgtggac ctgctcagcg
     3061 aggtcgccat gcccaaccag gaggagctcg cggcgaaggc agccgatcgg atccacctcg
     3121 tgcacgagac ctacggcaat gcccagcaag gctggttcgt gccgacctat gcgactcagc
     3181 cgggccagcc gctcgccggg ctgcgcagcg tcacccagct caacgactac gcggtcgcgc
     3241 tcggggggaa gttgatcgac gccgatccca gcttcatcac gacgaagcag aacgccgcgc
     3301 ggctggccgg ctacggcctg cggctcgaac aggtgacctc cagcgaggcc gcccagctcg
     3361 ccgagctgcg ccgcgcctac gagcgccgcc agccgattct cgtgtacctg taccacccgc
     3421 actgggcgtt cgaggtgttc gacctgaccc agctggagga gcccaacccc taccagcccg
     3481 actgcttcac caccgggaac ggggcctgcg ccatgcctgc ctatgccgcc tggaccgctg
     3541 ccagcacgga tctgcagcgg ggcgcgccgc ggttccacgc gatgctcgag cgcttcgagc
     3601 tgccgctacc cgacgtcgag gcgatgctca aggccgtcga tgtggacgac gccgacgtcg
     3661 acgaggtcgc tcggcggtgg gtcgccgacc accccgctgc tgtccgttcg tgggcgagcg
     3721 catgaacgcg ccggcccggc ccatgatccg gtgcaaacgg gtcaccaagg tgttcgggcc
     3781 cgcggcggcc ggttccgggc cggctgtcga cgaggcgctc gccatggccc acgacggcgt
     3841 cgggcgcgcg gagatccagc agcgcaccga cgcgacgctc gcggtgtacg acgtgtcctt
     3901 cgacgtgctg ccgggggagc tgttcgtcgt gatgggactg tccggctcgg gcaagtccac
     3961 gctggtgcgg ctgctcaacc ggctcatcga gccgagcagc ggcgtgatcg aggtcgacgg
     4021 ccgtgagctc ggctccctgg acgacaccga gctgcgcgag ctgcgcaacc ggcgcatgag
     4081 catggtgttc cagcacttcg ccctcctgcc gcaccgcacg gtgcggcaga acgcggagta
     4141 cgggctcaag atccgcgggg tgcccgacga ggagcgccgg gagaagggcg agtgggcgct
     4201 ggagcaggtc ggcctcgccg accgggccga ggcccacccc ggccagctct ccgggggcat
     4261 gcagcaacgc gtcgggctcg cacgggcgct ggccagcgat accgacgtgc tgctgatgga
     4321 cgagccgtac tcggcgctcg acccgctgat ccgccgcgac atgcaggcac tgctcgcccg
     4381 gctgcagtcc gagctgcgca agacgatcgt gttcatcacc cacgatctca acgaggccat
     4441 gctgctcggc gaccggatca tgctgctcaa ggacggtcgg ccggtgcagg tcggcaccgg
     4501 ccccgagatc ctcgccgcgc ctgccgacga ctacgtcgcc gacttcgtct ctgacgtcga
     4561 tcgcacgcgc gtgctcacgg cggcggacgt gatgcgggag gcgcgggtgg tggtccgccc
     4621 cgacgagcgg cccgccgacg tgctgcggcg cctcggcgag tccgaggcga ccggtgccta
     4681 cgtgctcgac gacgatcggc gcatcctcgg cgtcgtccgg gacgactggc tggcgcgcgc
     4741 ggccgccacc gggaccgact cgatctcccg ggccggcctc gacggcgact acctgaccac
     4801 cgagcccgac cggccactga tcgacctcgt cgaccaggtg ggccgcaacg tcgtcccgct
     4861 ggccgtcgtc gacgaggggt cccggctgct cggcgtggtc ccgcgggcgg ccgtgctcgc
     4921 gtcactggcc gcccccggca ggagctgacc atgccccgca tcgagatcgg tacctacgtc
     4981 gagcagttca tcacctggct gctcaccgcc atccccggcg tgctcgacgg gatcagcgcg
     5041 gtcgtcacgg tggtcgtcga cgcgctcacg gcggtgctgg ccggtccacc gttcgtcgtc
     5101 tgggtgctgc tgctgaccgt ggcggccctc ctcgtgcgcg ggtgggcgtt tgccgtgttc
     5161 accctggtcg cgttcctgct gatcgccggg ctgaacctgt ggcaggagac gatggagacc
     5221 ctcgcggtcg tgctcgtcgc cgccgtggtc gcgaccgccg tcggtgtgcc actggggatc
     5281 tgggcggcgc gcagccgcgc tgtcggaacg gtgctgcggc cggtcctcga cctcatgcag
     5341 accaccccgg ttttcgtcta cctgatcccg gccgtgttct tcttcggcat cggtgtggtg
     5401 cccggcgtgg ttgccaccac aatcttctcg atcccgcccg cagtgcgcct cacggagctc
     5461 gggatccgcg gggtggaccc ggaggtcgtc gaggcggccc acgcgttcgg ggcgcatccg
     5521 cggcagatcc tgcgcgaggt ccagctgccc atggcgctcc cgtcgatcat ggcaggggtc
     5581 aatcaggtga tcatgctggc gctgtccatg gtggtcgtcg ccgggttggc cggggccgga
     5641 gggctcggca cggtggtcgt gagcgcggtg acgcagctcg acatcgccgc aggcgtcgac
     5701 ggcggcctcg ccgtcgtgat cctggcgatc ttcctcgacc gcttcaccgc ggcgctcgcg
     5761 gagcgcccga accgggggat gtggggaagg ctccggagcc ggcgtgccgg cgcggccacc
     5821 acggcgccgc gcgtcgccga gacacgcacc cgcgccgagg acccggtgcc cgcacccggc
     5881 ctgtcctgag ccgctgaggc accggcgagc aggtcgcccg cgtcgcccgc cgcccaggcg
     5941 ggcggtgagc cgaatcaggt cattgagccc gctgtagccg cgccccgtgc cagccctcag
     6001 cgcgcggcgc cctcgggcag ggcgccgtgc agcagcgctg cggcgtcgtc ccagcccagg
     6061 gtcagcaccg tcgcggcgag ctccggtccg acgcatcctg cgaccttggc cgacaggtcg
     6121 gcgtccgaca acggtcggcg tgggtgccca tcgggcacgt ccagcaccgc gtgctcgacc
     6181 gtgccgtcca tgagctccac ggtgatccgg acgtcgccgt cgaggatgcc gatcccaccc
     6241 ggctcgggcc ggatgtcgac gagcccggcc agctgcttcg cctcaggacg gccgactgcg
     6301 gcgtcggtga agctggcgag gccgggaaag ccgtcgagaa gcgcgaccgc taccgcgtac
     6361 tcgagcgagc gggttgcctc ctgaccggtg cggggagcgc tccggatcag cggttggagt
     6421 gccgactgcg ggatgtgcac gcggatggct gcgatctggt cgcggggcag cggtgccagc
     6481 aaccggacgg cgctgatcac gcgctgcagc gcgtcgctgc aggggtaggg cttcaccgtg
     6541 agcccgtccg gcacgagggg cccgtcgagc gcgtccactc cgccgatcat ggcgaaccac
     6601 tggtcgagcg ttgcggggtc gacgggcgcg cccgccgcgg ccaggtgggc ggcccgcacg
     6661 cccgcggccg cggccaggcc cacccgcagc ggcctgtccg gggcgccgca ggagccctgc
     6721 acgccgctcg cggccggcac cgcgagtgcc atcgccgtgg ccagcgcggt ggcgtcgagc
     6781 ccgagtgcca gcccggcggc gacggccgcg gcgggtgccc cgacggtgca cgtggcgtcc
     6841 catcccgccc ggtcgtgctc ccggtcgagc gctgcgccga gccgcgccat caccccggcc
     6901 gcggccaggt actcccggct accaccaccg caggccaggg tggccggcac gcagaccacg
     6961 ctcacccgcg aggacgaggg caggtgcaca tcgtcgatgt cgagaacgtg cccgaccgcc
     7021 gcccagcgca gtaccgcggg cgacccggcc gtccagccgg tgtccgggcc gtcgacggcc
     7081 gcgagtgtgc ccgccacggt gtccacgagt gctcgccggg ccagctcctc gtcacccggg
     7141 gtcgggcgga cgcctgcggc ccactcggcg aggtcggcga tccggagacc cccagggcgt
     7201 ccacgcccgg tgcggcactg ctccgggccg cgacgacggt cacgacgtct acgcgcacga
     7261 accgaggtcg ggctcgcgtc gcgtcgtgcg gtcatgccgc cgtccgtcat cgaatccccc
     7321 gatcgtgccg gcctccggca catgtgccgc actacggtcc ggccgtgtgc cacgatcggt
     7381 gaccaacttc ggcactgtca agggggacct cggatgtcgg caccaccacc ggatacgggg
     7441 atgcgcaccg tgctcaccac gcttcgtgtg ttggaggtgg tctccgagct gcagccggtc
     7501 ggggtctccg cggtggcacg ggccaccgga attccgaaga gttcggtgca acgatgcctg
     7561 gtcaccctgg gccaggccgg ctggctcacc caggcgaagg aggacacgca ccattgggtc
     7621 ctcaccggcc gggcgctgtc gatcgggatg cgcggctcca cggagaaggg gttgcgggag
     7681 gcggcactgg ccgtcatgca ggacctgcgg gacgagaccc gcgagaccat ccacctactc
     7741 ggcttcggcg aggagcacgc tcccgccggg ggtgtcgagc ccgccaccga cccggcgctg
     7801 gtggtgatcg accggctgga cggcccggag ccggtccgga cctgggtccg gctgggcacg
     7861 cgcgttccgc tgcacgcgac ctgcgcaggc ctcgcggtac tcgcccgcct gcccggcgcc
     7921 gaggtggacc gcctgctccg cgggcggctg gagcgctact ccgaccacac gatcgtcgat
     7981 cgcgatgcgc tcgccgccga gctgcgggac gtgcgcgagc gcggctatgc gatggccgac
     8041 cggagctggc gccgcgggat cggggcggtg gccgctgcgg tcatcgacgc cgcgggcgac
     8101 ccggtcgcgg cgctcgtgat ctcgaccccg atgcagcggt tcgacaccga ccgcgcccgc
     8161 gttctcggac cgctggtggc cgcggcggcg tcgcgggtca gcaacgatct gcgattctga
     8221 gttctccgac ggggtgtccg aaagtccgca ccgcggtccg ccggttggtg agacctggca
     8281 ggctggcctg cggaacggtt tcctggaggg tgacgtgagc agcgacgacg accgcacaga
     8341 cgggtacgac aacgactccg ggctgctcga gcccgaggac agccttgacg accgcggtgt
     8401 cgaggacgtc ctcgacgagg gctactcacc gccggagcgc ccgttgggcg tggacgagtg
     8461 gggcaccacg ccgcgcgagg agcacgacgg cgagggcttg gagcgcaggc tcgcccggga
     8521 gctgcctgac ggcggcacgg ccgagtggga cggcctgggc gacgcaggcg acaccgacgg
     8581 cgagctgatc gacgacgagg tgggaaccgc ccgctccggt cggctcgtgg cttccgacaa
     8641 cggcgggttc tccgacgagg acgacgagct ctacgcggag gacgtaggtg tcgacggcgc
     8701 cgcggcctcg gcggaggagg cggcggtcca cgtcgtcgcc gaccgggacg acgacgagac
     8761 ctgagaatta tcgtccggct gacagatcga cccgctatgg cgaggttcct ttgcccggct
     8821 ggcgagcctg gacctgaacc tgctggtcgc gcggtgtgag ctgatccgcg agcgacccgc
     8881 gccggtgcgg gtcgaccgtc ctggtgtggt tcggtcgcgc tcgatcagcc cggtgcgtcg
     8941 tcgaggtcgc ggcccggcat cgactgtccc gcgctctccc ggctgccggc ccgcgtccgc
     9001 atcgggtcgg gtgcgctgtc ctcggggaag cggaggagga actcgcgttc gagcgcgagc
     9061 atccgttcgg tgtgggctcg gagcgccgac tcgctgcccc cgagcagcgt gtcgtgccgg
     9121 gtctcgtgca ggtgcgccac ctcacggcgg aggtcgtcgt cgtcgagttc ctggggagcg
     9181 atgccgtgtg atcgatctgt catgcaggag gagtgcccac gcagcggatc gagcattccc
     9241 ccggttgtca gagaaccggg tgacgcccgg tccgggccgt cgtccagaat agccgctcgg
     9301 cgaccagggg gcgggtctgc agtggaccga ccggaacgat cacgaagact gctcgcggcc
     9361 caggctgccg cgcctatgcg ggtgcaggcg ccggtgcccg gcgggtggcc tggtgacgtg
     9421 atcgggaccg gagacgaggc cggagtgccc gaggcacccg ccgggctcgc cgacgccctg
     9481 gtagaggccg tgctgagtgc gcttcccacg gtggctgccc gcatccccgc agcgcccgtg
     9541 gccgcgctcg gcaggctcgc ggcgccgatc gccccggccg acccgcttcg acccgcgccg
     9601 cagcgccggt ccggggtgga ccacctgctc gccgagcggc tgctcggcgc gctggagctc
     9661 accgtccccg acgtgcggct cgacgttccg gacgtcctcg tccagcacgt gcgcgcgccg
     9721 cttccgcagc cgacgggcgc cgtggccggc atggcggcgg gtggtgagag cgagacccgc
     9781 actgcggtcg tcctgctcga ccaggtcgcg cccggtctgc gcgagctggt caccggcctc
     9841 gtcggcgcgc tggcggagca cgagcgggtc gccccgctgc tggaggcggc cccgggcgcc
     9901 gagccggagg tcgcggcggc gcacggcgga gtgcacctcg ccctcggcgt cgtcacggcg
     9961 ctggcggtgc tgcgggcggc tcggctcccg attgtcgggg cggagccgcc ggtcgtggtc
    10021 ggggtggcgc tcgacgcgac cctgcgggtg ctgcggcagc gcccgatgcc cgccgcgtac
    10081 gccgccgcgc tgcgcgagcg gctgcgcgac gagtacgtgg tcccgcggtt ctccgcaggc
    10141 gacgttcccg tgcaggagca ccgcttcgcg atggtcgagg gaccgttccc gccgctggcc
    10201 ggggagcccg tgaacgggct ggtcgaggcg atgcccggcg ggatcgtcgt ccgtaccgga
    10261 gtgcggggcg ccgcggtgcc ggtgtggctc agggtgctgt ccgaggagcc cgccgtggtg
    10321 gacctggcca cgtggcacga ggtcgtcgac gtcagctaca ccgcggtcga gggggcggcg
    10381 tcggtgctcg gtcccgctgc gcctgcgcct gcgcacctcc tgggccagac gccgccctgg
    10441 ccgggaccgg tccgcgcgcg cgtgtcggcg cgcgggcgcg acgagcacgg cgacggcagg
    10501 gagggtggcg agcgctacga gatcgtggtc tggccggcgc cgctcgcgcc ggaggtggtg
    10561 cacaaggcga ccgaccggct cggccaccgg ctgcgggggg agccggagcc gccggtcgtc
    10621 gtccggccgg aggtggccta ccgctgggtc cgcggcaccg gtatcggcga ggcggccacg
    10681 gtcaccgtcg tcaccgggtc gggcgcggag gaggtgctgg cggcattcgg cgccgacccg
    10741 gcggacccgg tgccgctcgc cgagctgttc gaccggtgcg ggatcgaccc gtgggtcgcc
    10801 gtgctgccgg tcgacggtgc ggtcctcgct ctcgagtaca acggctggca gggggcacag
    10861 ggcccggtgc tccggccggc gtccgcacgc ggacgcgccg ccagcatgta ctggaacgtc
    10921 aatgcgcttt cgagcctgtc ctttgcccgc ggcggggagg tgctggcctc cttcgagcct
    10981 ccgccgccca cccga
//
LOCUS       DASRSC010000339         9270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_305548,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000339 DASRSC010000000
VERSION     DASRSC010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9270
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305548"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /gene="tsaB"
                     /locus_tag="VFE39_16490"
     CDS             complement(<1..714)
                     /gene="tsaB"
                     /locus_tag="VFE39_16490"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET6260237.1"
                     /translation="MLVLALDTATPAVTAGVVELTGAGPVERSVRVTHDARKHAELLM
                     PGVLAACSDARVALRDVDAVVTGTGPGPFTGLRVGMVTAAALGDALGVPVHGVCSLDA
                     IAADDFRGAAPGGPLLVVTDARRREVYWALYAALRDADFPDAVTRIDGPHVEAPAALA
                     DRVAELGAVSAAGGSAGLVGLPVREPASPSPAGLVAMAAAALRSGAVPGPLEPLYLRR
                     PDAVEPRARKPVSAPPSRGA"
     gene            complement(892..1566)
                     /locus_tag="VFE39_16495"
                     /pseudo
     CDS             complement(892..1566)
                     /locus_tag="VFE39_16495"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
     gene            complement(1605..2027)
                     /gene="tsaE"
                     /locus_tag="VFE39_16500"
     CDS             complement(1605..2027)
                     /gene="tsaE"
                     /locus_tag="VFE39_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET6260238.1"
                     /translation="MPAAADTEALGERLAAGLGAGDLVVLSGPLGAGKTVLVRGLARG
                     LGVAGPVTSPTFVIAREHRPLPGGVGVPLVHVDAYRLGGAAELDDLDLDTDLADAVVA
                     VEWGEGVAERLSARHLLVRLTRRPDDVRVASVHRVEAA"
     gene            complement(2069..3175)
                     /locus_tag="VFE39_16505"
     CDS             complement(2069..3175)
                     /locus_tag="VFE39_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6260239.1"
                     /translation="MLNRGQIWGGIAGAAGAAVTGVAVGVAARKRSQVAADRRRLATQ
                     LSESAPGPGVTAVGESSSVTADDGVRLSCEEIEAPDGNAALTVVLVHGFALDRRTWHF
                     QRRTLPELADPTLRLVLYDQRSHGRSERAPRESCTIEQLGHDLDAVIRALAPEGPLVL
                     VGHSMGGMTIMALAEQNPELFAERVAGVALVSTSVGEVASAGLHGALLSRHNPLTRGV
                     GLLARLQPKLVEGVRKVAGEVIWAITRSLAYGDRNVEPWLVDLVDTMISTNAVDALTD
                     FVDTVGSHDRIAALPALATCEVLVAAGDADRVIPVSHSDVIAAELPDATLVRFEGAGH
                     LPMLEQPLAMDAALVDLLRRSAARSRPRRLRRRA"
     gene            complement(3169..4314)
                     /gene="alr"
                     /locus_tag="VFE39_16510"
     CDS             complement(3169..4314)
                     /gene="alr"
                     /locus_tag="VFE39_16510"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET6260240.1"
                     /translation="MPGSSRAETVVDLDAVRHNINVLRAAASGAGVMAVVKADGYGHG
                     ASTVARAALDAGAGWLGVCTLEEAMDLRDAGITAPLLSWLHLPDDDFAEAVAAGVDLS
                     VSSRRHLAGVLAGGRRAGRPVRLHLKVDTGLGRNGAQPTEWADLLDDAAKAAADGAAE
                     VVAVWSHLAHADAPHHPTIDRQAARLTTAWQEAKDRGLAPIRHLANSAATLTRPDLHF
                     DLVRPGIAVYGLDPLDRQPGQSPLRPAMTLRSRVALVKRVPAGEGVSYGHEWTTPRET
                     TLALLPIGYADGVPRRLGREGRMRVLLGGVLRPVVGRVCMDQVMVDCGDDPVREGELA
                     VLFGPGDHGEPTAQDWADELGTIHYEIVTGVHGRRVTRTVAGGGGTC"
     gene            complement(4385..5827)
                     /locus_tag="VFE39_16515"
     CDS             complement(4385..5827)
                     /locus_tag="VFE39_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET6260241.1"
                     /translation="MRGVWTAQQVRDAEAVLLDRTPGDTLMRRAAFGLAVHARRMLAR
                     EGGVAGRRVVLLVGGGNNGGDALWAGVELRRRGVGVTAVLLDAARAHPAGCAALRRAG
                     GRFVDPDAGLVAEFGADLVIDGIIGISGRGALREPAARLVAAVDHAGVPVLAVDLPSG
                     LDPDTGAVDGPAVTATATVTFGALKPVHVLAAHRCGEVHLVDIGLGPVLPEPHAQVLE
                     AADVARRWPIPGPEDDKYTQGVVGIAAGSATYPGAAVLATGAATLATSGMVRFAGSAA
                     PLVRDHWPEVVITESISDAGQTQAWAVGPGIGTDDAGRAVLAEAIARDVPLCIDADGI
                     TLLAAHADLRDALRGRPVVLTPHDREFARFAGEVGTDRIGAARRAAAELDVTVLLKGN
                     ATVVAESGGRAFVHPATSSWAATAGSGDVLTGIIGALLAAGLEPWWAASCAAHVHGLA
                     AALAARGAPAPASRLQAAIPEAIRAVRAAG"
     gene            6040..6867
                     /locus_tag="VFE39_16520"
     CDS             6040..6867
                     /locus_tag="VFE39_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6260242.1"
                     /translation="MTRTRPFSRPPEARNAKEAVEQLSRPGPHRPVRGDLGMVGIPGV
                     VFAPEEGLGLPAVAFGHDWLQPAERYADLLRHLASWGIVAAAPSSHRGPLPSHAGFAA
                     DLRTTLDICAGVRLGDGRISVDSRRTALAGHGIGGGVALMAAQDRPRVRAVVTIAVAQ
                     TRPSALDSARALTIPVLHLAAGKDTVAPAAGHAEPIAASAGGPVWLRTLSKASHTGFL
                     EGVHWSDFVLSGSPDAKTRRVTRALVTAFLLQHLCDEDRVEMLIDGKVPGTTLAERH"
     gene            complement(7062..7466)
                     /locus_tag="VFE39_16525"
     CDS             complement(7062..7466)
                     /locus_tag="VFE39_16525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260243.1"
                     /translation="MTPLVPPPDPDALHLIARSCAGLGDALAEVGRRVRGLSEQIAHD
                     WPDGRGHEWVQRTARLGHELGREAAAAVELGEAYARHAADPPAWGALPPMVGPAARHA
                     GVRLGGTEAQRVDEGRGMRIAQLPDDASTEPN"
     gene            complement(7463..7774)
                     /locus_tag="VFE39_16530"
     CDS             complement(7463..7774)
                     /locus_tag="VFE39_16530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260244.1"
                     /translation="MSSELRLQPDRLRSHAVAAASLSEELRGLLPVMPPPGAPGRPAL
                     AAEHERLQAAVLVAIRELAELSAAVAGAATAAVAADGAVARVLRHVEEGLGPSQGGER
                     V"
     gene            complement(7824..9161)
                     /gene="glmM"
                     /locus_tag="VFE39_16535"
     CDS             complement(7824..9161)
                     /gene="glmM"
                     /locus_tag="VFE39_16535"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010316185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET6260245.1"
                     /translation="MRRLFGTDGVRGLANGELLTPELAVALSGSAARVLAERDRSHRP
                     IAVVGRDPRASGEMLEAAVVAGLTSAGADAVRVGVLPTPAVAFLVGALDADLGVMISA
                     SHNPMPDNGIKLFASGGHKLPDALEEAVEAGVAAPAQRPTGAGIGRVRDLADAAERYR
                     DHLVAATRASLAGLRVVVDCAHGAASTVAPEAYRRAGADVIALHADPDGLNINDGVGS
                     THIGPLQKAVREHGADLGIAHDGDADRCLAVDATGAEVDGDQIMAVLAVAMQEAGELA
                     HDTLVTTVMSNLGLHLAMQAAGIAVRITQVGDRYVLEELRTGGFSLGGEQSGHIVLPQ
                     HSTTGDGLLTALRLMARMTATDSSLAALTAIVTPLPQVLRNVHVVDKAAVADSAAVRD
                     AVEAAQVELGGTGRVLLRPSGTEQLVRVMVEAPTAELAEATAVRLVEVVAAAR"
     gene            complement(9203..>9270)
                     /gene="rpsI"
                     /locus_tag="VFE39_16540"
     CDS             complement(9203..>9270)
                     /gene="rpsI"
                     /locus_tag="VFE39_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET6260246.1"
                     /translation="VIERKKYGLKKARKAPQYSKR"
BASE COUNT         1240 a         3531 c         3378 g         1121 t
ORIGIN      
        1 cgcgcctctg ctcggcggtg cgctcaccgg cttgcgggct cggggctcca cggcgtcggg
       61 acggcgcagg tagagcggct ccagcggtcc gggcacagcg ccggatcgca acgcggcagc
      121 cgccatggcg acgagccccg ccggcgacgg cgatgccggc tcccgaacgg gcaacccgac
      181 cagcccggcc gagccgcctg cggcggagac ggcgcccagc tcggcgacgc ggtcggccag
      241 cgccgcaggg gcctcgacgt gcggcccgtc gatccgggtg acggcgtcgg ggaagtcggc
      301 gtcgcggagg gcggcgtaca gggcccagta gacctcgcga cgacgggcgt cggtgaccac
      361 gagcaacggg ccgccgggcg ccgcaccgcg gaaatcgtcg gccgcgatcg cgtcgagcga
      421 gcagacaccg tggaccggca caccgagcgc gtcgccgagc gcagcggcgg tcaccatgcc
      481 gacccgcagg ccggtgaacg ggccggggcc ggtgcccgtg accacggcgt cgacatcgcg
      541 aagggcgacg cgggcatcgg agcaggcggc gagtaccccg ggcatgagga gctcggcgtg
      601 cttgcgggcg tcgtgggtga cgcggacgga ccgttcgacc gggcccgcgc cggtcagctc
      661 gacgacgccc gccgtgacgg cgggcgtcgc ggtgtccagg gccagcacga gcacagcccc
      721 gagcctacga gcaggtccga cggccgaatg gacggggcac cagccctgtg atcggatcga
      781 gactcgacga gcggcacgtt tgtgcggacc tgttggacgg ggcggccgtt cctgcggtgg
      841 ggggcgcgag cggtgctgtg cggcgcacct tgctcaccgc gctcgggccg ctcaccacga
      901 ggcgagcagc tccgggaggt cggcgaggcg ctggatcacc ccgtcgggct gcacgtccac
      961 cggcacctgc tgcacggctg gaatgtccga gtgcggcacc agcaccgcgc gcatccccac
     1021 gcccttggct ccattgatgt cgtcgtaggg ccggtcgccc atgtacacgc agcggcccgg
     1081 atcggtgacg ccgacggctg ccatcgcggc acggaacgcg gtggggcgcg gcttggtcca
     1141 cgggagctcg gtggtccaca cgcacgcgtc gaagtgctcc agcacgccgt cgcgccggaa
     1201 gagctcctcg tgccattccc gtggccacgc cgtggacgac agcatgccga gcttcagccc
     1261 acgctcgcgc agcgcggcga gcatcggggc cgcgtccggg tcggtggctg tgaactgcac
     1321 ccagtactcg cggtacgcct cgagcgcggc ctcgtaccgt tcgcgcgggt ggtcctcggc
     1381 atccgcggca tcggccagca cctgggcgag cgtgaacgcg cggtgctcct cgtgcacggt
     1441 catccagcgg gcctcgtcgg cggcaaggag cgcggccgcg agcacggcgg cgcggtcggg
     1501 ctcgtcggca tgcagcacgt cagcgagcct gcgccaaccg gcgaggtgat cgaccgtggt
     1561 ccagggggtg agcgtgccgc ctcagccgac gatcaccgcg tcgatcacgc cgcttccacc
     1621 cggtgcacgc tcgccacccg cacgtcgtcg ggacggcggg tgagccgcac gagcaggtgc
     1681 cgcgccgaga ggcgctccgc cacgccctcg ccccactcga cggccaccac ggcgtcggcg
     1741 aggtcggtgt ccaggtcgag atcgtccagc tctgcggccc cgccgagccg gtaggcgtcc
     1801 acgtgcacga gcggcacgcc gacaccgccc ggaagggggc ggtgctccct ggcgatcacg
     1861 aaggtcggtg acgtgacggg cccggccaca ccgagcccgc gtgccaggcc gcgcacgagg
     1921 acggtcttgc ccgccccgag cggcccggac aacaccacca ggtcgccggc tccgagcccg
     1981 gcggccagcc gctcgcccag cgcctcggtg tcggcggccg ccggcagctc cagctcgacg
     2041 tcgagctcga ccgagcgctc caccggggtc acgcgcgccg ccgcagcctg cgcggcctgc
     2101 tccgcgcagc ggagcggcgg agcaggtcca ccagcgcggc gtccatggcc aggggctgct
     2161 ccagcatcgg gagatgcccc gcaccctcga agcgcacgag cgtggcatcc ggcagttccg
     2221 cggcgatcac gtcgctgtgc gagacgggga tcacgcggtc ggcgtcgcct gccgcgacga
     2281 gcacctcaca ggtcgcaagg gctgggagcg cggcgatccg gtcgtggctg ccgacggtgt
     2341 cgacgaagtc ggtcagcgcg tccaccgcgt tcgtactgat catcgtgtcg acgaggtcga
     2401 ccagccatgg ctccacgttg cggtcgccgt aggcgaggct ccgcgtgatc gcccagatca
     2461 cctcacccgc caccttccgg acgccctcca ccagcttcgg ctgcagccgg gccagcagtc
     2521 cgacgccccg ggtgaggggg ttgtgccggg acagcagggc gccgtggagc ccggcgctgg
     2581 ccacctcgcc caccgaggtg gacaccagcg ccacacccgc gacgcgctcg gcgaacagct
     2641 cggggttctg ctccgcgagg gccatgatcg tcatgccgcc catcgagtgc ccgacgagca
     2701 ccaggggtcc ctccggcgcg agcgcccgga tcaccgcgtc gagatcgtgg ccgagctgct
     2761 cgatcgtgca gctctcccgg ggagcacgct cggagcgtcc gtggctgcgc tggtcgtaga
     2821 gcacgagccg cagggtcggg tcggcgagct cgggcagcgt gcggcgctgg aagtgccagg
     2881 tgcggcggtc gagcgcgaac ccgtgcacga gcacgacggt gagcgccgcg ttcccgtccg
     2941 gcgcctcgat ctcctcgcac gacagccgca caccgtcgtc cgcggtgacc gaggatgact
     3001 cgcccactgc cgtcacgccg ggaccggggg cactctcgga gagctgcgtg gcgagccgcc
     3061 gccgatcggc ggcgacctgc gagcgcttgc gggcagccac cccgacggcg acaccggtga
     3121 cggcggcccc cgcggccccg gcgatcccgc cccagatctg cccccggttc agcacgttcc
     3181 gccaccgccg gcgacagtgc gggtgacccg gcgcccgtgc acgccggtca cgatctcgta
     3241 gtggatggtg ccgagctcgt ccgcccagtc ctgggcggtc ggctcaccgt ggtcaccggg
     3301 gccgaacaac acggcgagct ccccctcccg gaccgggtcg tccccgcagt ccaccatgac
     3361 ctggtccatg cagacgcgcc ccacgacggg ccgcagcacg ccgccgagca acaccctcat
     3421 ccggccctcg cggccgagcc tgcggggcac gccgtccgcg tacccgatcg ggagcagcgc
     3481 gagcgtggtc tcgcgcggcg tcgtccactc gtgcccgtaa gagacgccct cccctgcagg
     3541 cacacgcttc accagcgcca cgcgcgaccg cagcgtcatc gcgggccgca acgggctctg
     3601 tcccggctgg cggtccaacg ggtcgagccc gtagaccgca ataccgggac ggacgaggtc
     3661 gaagtgcagg tcgggccggg tgagggtggc cgccgagttg gcgaggtgcc ggatcggggc
     3721 cagaccccgg tccttcgcct cctgccacgc ggtcgtgagc ctcgcagcct gcctgtcgat
     3781 cgtggggtgg tgcggcgcgt cggcgtgtgc gaggtgcgac cagaccgcga cgacctcagc
     3841 ggcaccgtcg gcggcagcct tcgcagcgtc gtcgagcagg tcggcccatt ccgtgggctg
     3901 ggcgccgttc cggcccagcc cggtgtcgac cttgaggtgc agccgcaccg gccggccggc
     3961 acggcgcccg cccgccagca ccccggcgag gtgccgccgg gaggagaccg acaggtcgac
     4021 ccctgccgcc accgcctcgg cgaagtcgtc gtcgggcagg tgcaaccagg acagcagcgg
     4081 tgcggtgatc ccggcgtccc gcaggtccat ggcctcttcg agcgtgcaga cccccagcca
     4141 gcccgccccc gcgtccagcg cggcgcgggc gaccgtcgat gccccgtgac cgtagccgtc
     4201 ggccttcacc acggccatga cacccgcgcc ggaagccgcg gcccgcaaca cgttgatgtt
     4261 gtgccggacc gcgtccagat cgacgacggt ctcggcccgc gaactccccg gcagatcgac
     4321 cacgcccgca tcatcgcatc cgcaaccacc ccatcgggga cggatagcag tcttcggctc
     4381 ggcttcagcc cgcagcccgg accgcgcgga tcgcctccgg gatcgccgcc tgcaacctcg
     4441 acgcgggcgc gggcgcaccc cgcgcggcca gcgccgccgc gagcccgtgc acgtgagcgg
     4501 cgcagctcgc tgcccaccac ggctcgagcc cggcggccag cagcgcgccg atgatcccgg
     4561 tgaggacgtc gccggagccg gcggtggccg cccaggacga cgtggcgggg tgcacgaacg
     4621 cgcgtccacc cgactcggcg acgacggtgg cgttgccctt cagcagcacg gtgacgtcca
     4681 gctcggccgc ggcccgacgc gcggcgccga tccggtcggt gccgacctca cccgcgaagc
     4741 gggcgaactc gcggtcgtgc ggcgtgagca ccacaggcct gccgcgcagc gcgtcgcgca
     4801 ggtcggcgtg cgccgcgagc agggtgatgc cgtcggcgtc gatgcagagg ggcacgtcgc
     4861 gggcgatcgc ctccgcgagc acggcccggc cggcgtcgtc ggtaccgatg ccgggcccga
     4921 ccgcccatgc ctgggtctgc ccagcgtccg agatcgactc cgtgatgacg acctccggcc
     4981 agtgatcccg gacaagggga gcggcgctgc cggcgaaccg gaccatcccg ctggtcgcga
     5041 gggtcgccgc gccggtggcc agtacggcgg cgcccggata ggtcgccgag cccgcggcga
     5101 tacccaccac accctgggtg tacttgtcgt cctccggacc ggggatgggc cagcgccggg
     5161 cgacgtcggc tgcctcgagg acctgagcgt ggggctccgg cagcaccggc ccgagtccga
     5221 tgtcgaccag gtgcacctcg ccgcaccggt gcgcggcgag cacgtgcacg ggcttgagtg
     5281 cgccgaacgt gacggtggcg gtggcggtga cagccgggcc gtcgacagcg ccggtgtcgg
     5341 gatccaggcc gctggggagg tcgacggcga ggacgggcac gcccgcatgg tccacggcgg
     5401 ccaccaggcg ggccgcgggc tcgcgcagcg ctccacgccc ggagatgccg atgatgccgt
     5461 cgatcaccag gtcggccccg aactcggcca cgagcccggc gtccgggtcg acgaagcggc
     5521 cacccgcgcg gcgcagcgcg gcgcaaccgg ccgggtgcgc ccgcgctgcg tcgagcagca
     5581 ccgccgtgac accgacgccg cgcctgcgca gctccacgcc cgcccacagc gcatcgccac
     5641 cgttgttgcc cccgccgacg agcagcacca cccggcgccc ggccacccca ccctcgcggg
     5701 cgagcatccg gcgcgcgtgc accgcgagcc cgaacgccgc acggcgcatc agcgtgtcac
     5761 cgggtgtgcg gtccagcagc acggcttctg catcacgcac ctgctgggcc gtccagacgc
     5821 ccctcatcga ccgtccatcg ctggcatggt cggagcgtag gacgccggtg cccacccgcg
     5881 ccggacgact cgcgtcacca ggacgtcaga gcggatcacg accgaccgtt tccgctgacg
     5941 tccgctcgtc cccctcgccc ggccgcgatc gtgtgccgcg gcccctggcg ccgcgttgcg
     6001 ttcgcccacc ggacgtcgct aggactacgg tgcagcgctg tgacccggac ccgcccgttc
     6061 tcccggccgc ccgaggcgcg caacgcgaag gaagccgtcg aacagctctc tcggcccggc
     6121 ccgcaccgcc ccgtgcgggg cgacctcggc atggtcggca tcccgggcgt cgtgttcgcg
     6181 ccggaggagg ggctcggcct gcccgccgtc gcgttcgggc acgactggtt gcagcccgcc
     6241 gagcgctatg ccgatctgct gcgtcacctc gcgtcctggg gcatcgtcgc cgccgccccg
     6301 tcgagccacc gcggaccgct cccgagccat gccggcttcg ccgccgacct gcgcaccacg
     6361 ctcgacatct gcgcgggcgt ccgcctcggc gacgggcgca tcagcgttga ctcccgccgc
     6421 accgccctcg cggggcacgg gatcggcggc ggggtggcgt tgatggcggc gcaggaccgt
     6481 ccccgcgtcc gtgccgtcgt gacgatcgcc gtcgcgcaga cgcggccgtc cgctctcgac
     6541 tccgcccgcg ccctcacgat tcccgtgctg cacctcgccg cgggcaagga caccgtcgcg
     6601 cccgccgccg gccatgccga gcccatcgcg gcgtcggcag gcgggcccgt ctggttgcgc
     6661 acgctctcca aggcgagcca caccgggttc ctcgagggcg tgcactggag cgacttcgtt
     6721 ctgtccggca gccccgatgc gaagacccgc cgcgtcaccc gcgccctcgt caccgccttc
     6781 ctgctgcagc acctgtgcga cgaggaccgc gtcgagatgc tcatcgacgg aaaggtgccc
     6841 ggcaccaccc tcgccgagcg tcactgacgc gagcgcccgc aggcccgcgc cggcatgcgg
     6901 cgagcgacac gcgggtgcgg gctgggcacc ggaacgacga ctcggtgggg cgacgggcga
     6961 ctcgcgggcg ggaaacgggc gactcgcggg cggacgaccg gcgactcgcg ggcctgaaac
     7021 gggcgactcg cgggcgggcg gggggcgtcg gcggcgggcg gtcagttcgg ttctgtgctc
     7081 gcgtcgtccg ggagctgggc gatccgcatc ccgcgcccct cgtcgacacg ctgtgcctcg
     7141 gtgccgccga ggcgtacgcc cgcgtggcgg gcagcggggc cgaccatcgg cgggagcgcg
     7201 ccccacgctg gcgggtccgc cgcatgccgc gcgtacgcct cacccagctc gaccgcggcc
     7261 gccgcctcac ggccgagctc gtggcccagc cgggcggtgc gctgcaccca ctcgtggcca
     7321 cggccgtccg gccagtcgtg ggcgatctgt tccgacagcc cgcgcacgcg cctgcccacc
     7381 tcggccaggg catcgccgag cccagcacac gaccgggcga tgaggtgcag ggcgtcggga
     7441 tcgggcggcg gcacgagcgg cgtcacactc gctcgccgcc ctgcgaggga ccgagcccct
     7501 cctcgacgtg gcggagcacg cgcgcgaccg caccgtcggc tgccaccgcc gcggtggccg
     7561 ccccggcaac cgcggcgctg agctctgcca gctcgcggat cgcgacgagc acggcagcct
     7621 gcaacctctc gtgctctgcc gcgagggccg gtcggccggg ggctcccggc ggtggcatga
     7681 ccggcagtag cccgcgcagc tcctcggaga ggctcgcagc ggcgaccgcg tgggagcgca
     7741 gtcgatcggg ttgcaggcgc agctcggacg acatggcgcg atcgttccgc acccagcggt
     7801 ggcgccgggg cggaacggga gaatcagcga gcggcggcca cgacctcgac cagccggacg
     7861 gccgtggctt ccgcgagctc cgcggtaggg gcctcgacca tcacacgtac cagctgctcg
     7921 gtgccggacg ggcgcagcag cacccgtcct gtgccaccga gctccacctg cgcggcctcg
     7981 acggcgtcgc gcaccgcggc ggagtcggcg acggcggcct tgtcgaccac atgcacgttg
     8041 cgcaacacct gcggcagcgg cgtcacgatc gcggtgagcg cggccagcga cgaatcggtg
     8101 gccgtcatcc gcgccatcag ccggagcgcg gtgagcagcc cgtcgccggt ggtggagtgc
     8161 tggggcagca cgatgtgccc ggactgctcg ccaccgaggc tgaacccgcc cgtccgcagc
     8221 tcctcgagga cgtaccggtc gccgacctgc gtgatgcgca ccgcaatgcc tgccgcctgc
     8281 atcgcaaggt gcaggccgag gttgctcatc acggtggtga ccagcgtgtc gtgggcgagc
     8341 tccccggcct cctgcatggc aacggcgagc acggccatga tctggtcacc gtcgacctcg
     8401 gcgcccgtgg cgtcgaccgc caggcagcgg tcggcgtcgc cgtcgtgggc gatgccgagg
     8461 tcggcaccgt gctcgcggac ggccttctgc aacggtccga tgtgggtgga cccgaccccg
     8521 tcgttgatgt tcagcccgtc cgggtcggcg tgcagcgcga tgacgtccgc gcctgccctg
     8581 cggtacgcct ccggcgcgac ggtggacgct gccccgtggg cgcagtcgac cacgactcgc
     8641 aggccggcga gcgaggctcg cgtggcggcg accaggtggt cgcggtaccg ctcagcagcg
     8701 tcggcgaggt cgcgcacccg accgatcccg gcgccggtcg gacgctgcgc cggggcggcg
     8761 acacccgcct cgaccgcctc ctcgagcgca tcgggcagct tgtgaccccc cgaggcaaag
     8821 agcttgatcc cattgtccgg catcgggttg tgcgacgccg agatcatcac tccgaggtcg
     8881 gcatcgagcg ccccgaccag gaacgcaaca gcgggagtgg gcagtacccc gacgcgcacg
     8941 gcgtcggccc ctgccgacgt caggcccgcg acgacggcgg cctcgagcat ctccccgctg
     9001 gcccgcgggt cacggccgac aacggcgatg ggccggtggg accggtcgcg ttccgcgagc
     9061 acacgagcgg cagacccgga caacgcaaca gcgagctccg gtgtgaggag ctcgccgttg
     9121 gccagtccgc gaacgccgtc ggtgccgaac agacgtcgca cggtgaccga gcttgcgagg
     9181 ttcccggccg acagagccaa gatcagcgct tgctgtactg aggagcctta cgggccttct
     9241 tgaggccgta cttcttgcgc tcgatcacgc
//
LOCUS       DASRSC010000340        15897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2762025,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000340 DASRSC010000000
VERSION     DASRSC010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 15897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15897
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2762025"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VFE39_16545"
     CDS             complement(<1..393)
                     /locus_tag="VFE39_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET6260247.1"
                     /translation="MDAAVSPRPTAAIAWVALGVVYVLWGSTYLGIRLILADVPPLFA
                     SGARFLVGGSLLGLVVLAVAGRKAFRMTWQQLGTTALSGLLLPAWGNGLVTIAQQKVA
                     SGLAALLIAAVPLYIVVLRALTGDRPRPD"
     gene            555..1118
                     /locus_tag="VFE39_16550"
     CDS             555..1118
                     /locus_tag="VFE39_16550"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020637734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6260248.1"
                     /translation="MATATVRPAAVSDVEEIVRIQACTWEAAYAELVPAAALAQLRSE
                     AAYHAWTAAVGAGAGHEVLVATEGQWTVGFCAAAVAAPPEGSTAPTEPTLGPGAWAEV
                     SALLVEPRWGRRGHGGRLLATAAEALRGTGAVYGLAWVPESDEASRRFYARAGWEPDG
                     TLRVLDTGDGELREVRITGSLDLNLQP"
     gene            complement(1173..1928)
                     /gene="dapB"
                     /locus_tag="VFE39_16555"
     CDS             complement(1173..1928)
                     /gene="dapB"
                     /locus_tag="VFE39_16555"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET6260249.1"
                     /translation="MTENGIRVGVLGARGRMGAQVCAAVEAAPDLVLVAQIDEGDALT
                     GLVDAGAQVAVDFTHPGVALDNVRFCIEHDIAAVVGTSGFDEAKLDAVRGRLAERPGH
                     VLVAPNFGIGAVLSMQFARQAARFFESVEVIELHHAGKVDAPSGTAVHTAALIAEARA
                     EAGAGPLPDATTSELPGARGADIGGIRVHSVRLPGLVAHQEVVLGTTGETLTIRHDSF
                     DRASFMPGVLMAVRAVRERPGLTVGLDALLDLA"
     gene            complement(1956..2435)
                     /locus_tag="VFE39_16560"
     CDS             complement(1956..2435)
                     /locus_tag="VFE39_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HET6260250.1"
                     /translation="MSPETVIAALLIVVGIVGIVVPVLPGLILVLAGVAVWAIPRGDA
                     LGWTVLGIATAIVVLGSVAKYLLPGRRLRDSGVPGRTVAAGAVLGVVGFFVIPVVGLF
                     LGFVLGIYLAELVRLRGSGQAWLSTRQALAAVGWSIVIELATGLLAAAVWIGGLVVG"
     gene            complement(2523..3857)
                     /locus_tag="VFE39_16565"
     CDS             complement(2523..3857)
                     /locus_tag="VFE39_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HET6260251.1"
                     /translation="MTDVLVTPGAGALAPAPGVSRTVLPGGLRVLTETVPGVRSVSLG
                     IWIGIGSRDETPLQAGAAHYLEHLLFKGTRRRTAVDIAEQLDAVGGELNAFTEKEHTC
                     YYAHVLDTDLPLAVDVLADVVTDATMAPADVEVERGVVLEEIAMRDDDPEDLLGELFD
                     EALFGEHPLGRPVIGSEASVRGMSRETLHDFWRGEYTTPRMVVAAAGNLVHSEVVELV
                     AAALGGAAARAGSAPPVGPRRLERAALTAGPRLVLRPEDTEQVHIMLGAPGLSRHDPR
                     RTVLTVLNTALGGGPSSRLFQQVREQRGLAYSVYSAVTSYSDTGHVAVYAGCAPERLD
                     EVVTVVRGVLAEVAVNGLTASEVARAQGNLRGGLVLGLEDTPSRMNRLGRNELDHGRQ
                     RTIVESLDRISAVTQEQVADLARELLSAPLTAAVVGPYDKERDLPASLRSLT"
     gene            complement(3854..6136)
                     /locus_tag="VFE39_16570"
     CDS             complement(3854..6136)
                     /locus_tag="VFE39_16570"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET6260252.1"
                     /translation="MTDAQTTDPAENVHETTAVIDNGSFGSRTIRFETGRLARQAAGS
                     VVAYLDDETMLLSATTASKQPKEHFDFFPLTVDVEERMYAIGKIPGSFFRREGRPGTD
                     AILTCRLIDRPLRPSFVDGLRNEIQIVVTVLSLDPQDPYDALAINAASASTQLAGLPF
                     SGPIGGVRVALIDGQWVAFPQYEDLQRAVFDMVVAGRIVGDDVAIMMVEAEATTETIE
                     LVAGGAQAPTEDVVASGLEAAKPFIRSLCVAQQQLADVAAKPTGNFPTYPAYQDDVFE
                     AVAANASDDLAQALAIAGKQERESRLDEVKAAVLDKVGPQFEGREKEIGAAFRALNKK
                     LVRQRILRDQIRIDGRGITDIRPLSAEVEVVPRAHGSALFERGETQIMGVTTLNMLRM
                     EQQIDSLGPETHKRYLHHYNFPPYSTGETGRVGSPKRREIGHGALAERALLPVLPTRE
                     EFPYAIRQVSEALSSNGSTSMGSVCASTMALYNAGVPLKAPVAGIAMGLVIDEVEGQT
                     RYVALTDILGAEDAFGDMDFKVAGTSEFVTALQLDTKLDGIPSEVLAAALSQAKDARL
                     TILEVIGEAIDRPDEMSQFAPRVTAIKVPVDKIGEVIGPKGKMINSITEQTGADISIE
                     DDGTIYVGAADGPSAEAAIGMINAIANPQLPKIGERFLGTVVKTAAFGAFVSLVPGKD
                     GLVHISKLGQGKRIAKVEDVVKVGDKIRVEVTDIDNRGKISLIPVGEESENGTAPATE
                     GVDTGDAADIDAAAAEVSPS"
     gene            complement(6357..6626)
                     /gene="rpsO"
                     /locus_tag="VFE39_16575"
     CDS             complement(6357..6626)
                     /gene="rpsO"
                     /locus_tag="VFE39_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011559497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET6260253.1"
                     /translation="MALDAAQKKTILDTYGVHEGDTGSPEAQVALLTKRISDLTEHLK
                     IHKHDHHSRRGLLLLVGRRRRLLKYLASVDVARYRSLIERLGLRR"
     gene            complement(6867..7319)
                     /locus_tag="VFE39_16580"
     CDS             complement(6867..7319)
                     /locus_tag="VFE39_16580"
                     /inference="COORDINATES: protein motif:HMM:NF014849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HET6260254.1"
                     /translation="MRLFTALWPPAEAVAALAAVTAPAGSPSGWQAVDPATWHLTLAF
                     HGEADPGVFARRLDRAVGGVPGPRLRIAGAGAFPGVRWAGVEADVSGRLAAPVTAAGG
                     DPAAFVPHLPVLRKRRRPGQDADPDPPAPVGVAQRPVVASGGGAAGGQ"
     gene            complement(7323..7832)
                     /locus_tag="VFE39_16585"
     CDS             complement(7323..7832)
                     /locus_tag="VFE39_16585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET6260255.1"
                     /translation="MADAGAGPLGFRMAVNRDRQRELYGAPLGDRVRRLTSALGISQS
                     RLARTLGISPAMLSQLVSARRVKIGDPTVLARLLMLDQRCHGVRIRPEPRAVDALLAE
                     VARARWQWNAPRVPAPRDPQGPGSGTAAAVLRGIADPARLAAAAAALGPGFPELAEVL
                     RQAASRPGA"
     gene            complement(7898..8839)
                     /locus_tag="VFE39_16590"
     CDS             complement(7898..8839)
                     /locus_tag="VFE39_16590"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET6260256.1"
                     /translation="MQRWRGLEAVPTGWGRSVVTVGVFDGVHRGHQQLIGRAVERGRE
                     RGLPTVLVTFDPHPAELIRPGSHPARLTSLRRRADLVAGLGVDAFCVLPFTTELARTE
                     PAEFAHEVLVERLHAADVVVGCNFTFGRGAAGDVALLAQLGQRFGFGVEGLELITDDG
                     ITFSSTYIRACIDAGDVEAATAALGRPHRIEGVVVHGDRRGRELGFPTANLSTPPYTA
                     LPADGVYAGRFAMTTVDGGSRLLPAAISVGTNPTFSGRARTVEAYVLDVDEDYYGFEV
                     ALDFEHRLRGQERFDGVEALVAQMRRDVERTRELLGG"
     gene            8994..9863
                     /gene="truB"
                     /locus_tag="VFE39_16595"
     CDS             8994..9863
                     /gene="truB"
                     /locus_tag="VFE39_16595"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET6260257.1"
                     /translation="MNAVSSGLVVVDKPTGLTSHDVVARLRRILSTRKIGHAGTLDPM
                     ATGVLVCGVGRATKLLGHLALDTKAYAATVRLGVVTSTDDAQGEVVGTTDATGIDDAA
                     IAVGIAALTGAIEQVPSSVSAIKVDGKRAYARVRAGEEVVLAARPVTVSEFRLLARRG
                     GDLDVMVECSSGTYVRALARDLGSALGVGGHLTALRRTRVGPFTLENARTLEELEREP
                     RLTLPLDDAVRVAFPRRDLDAALAADISHGRPLPAAGVEGTYGVFAPDGRALALVSER
                     GELARPVVVLAPA"
     gene            complement(10032..10889)
                     /locus_tag="VFE39_16600"
     CDS             complement(10032..10889)
                     /locus_tag="VFE39_16600"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HET6260258.1"
                     /translation="MTDRAMMGAMRMGTTTAEVIGSRVIAAALGPSRPSARSGPLGPA
                     AGPTGAGPAVRALAGIGPRGPEVPGPAGAVEPLTPQGIVDVLARLQAGADAGRRGEGV
                     GPVNRLLSAVVGAAVDDVGADREFAAGMVTELAKRYLRAVRGHARGTPVPRAWQEVID
                     RWDPATADPGRIALAGGLTLVGLDLAPAVVSICTLLGRTPGPAERADVQTIIGLIAHR
                     LEAAGAGDPGAAADDAGLGLLLSHHDAWRQAEHLWALRGRPSEAELERAAMDWRAGLV
                     ARGLLAGPG"
     gene            11149..11730
                     /locus_tag="VFE39_16605"
     CDS             11149..11730
                     /locus_tag="VFE39_16605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6260259.1"
                     /translation="MTVSPAPTTISTLGNAELVELAARGDNRAWAEIVLRYRGLVTAV
                     VRSFRLQDADARDAEQRTWLRLVEHLRSLRDPDRLGGWLSTTASRECLRILREAQGVV
                     TVEMDAVPDPESGVEERVVDADTAARLWRIVAALPPRGRTIMNALFAEEPRPYAEVAQ
                     STGIPIGSLGPSRARLIDRVRRSFDEGAFATGA"
     gene            complement(11749..14763)
                     /locus_tag="VFE39_16610"
     CDS             complement(11749..14763)
                     /locus_tag="VFE39_16610"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET6260260.1"
                     /translation="MTERLASPPLVGREDDVAALTAALDEVQQGRMATVFLVGDAGVG
                     KTRLVQDLVSRADRANATVLLGGATDIAESPPFWPVASALRTLLRSPRGEEVQRQLSP
                     WADQLEELVALRPAVPALAGPQARVQTLELLHRVVLRLAGGSVVVLVVEDLQWADRST
                     LDLLAYLVANLADERVLVVATHRTELPTDASPARTMISELRRHRQVRAMEIMPLRRDA
                     VAEMVRALAPGRTDLVDLVWNRSAGNAFIAEETVRAALDGDPTGLPTTLRELVLSRVN
                     RLSEPALRAVRAIALCDGPLPHHLLEAVLGGGGPDLFDALREAVNAGVVVVDDGVDAY
                     RLRHGLMTDVVIRELLPGERVHLHRRYAMALEAAWTRELPGVDARLAHHWQSAGDVQR
                     AAAATVAAAEAAGRVRGYAEAHRHWLRAARLAGQLSAAPVPRSGCLERAAEAAHLAGD
                     ADQAVALLGERLRMAETPDEPGDLDEALLHARTGQYLVAAGRGAEAALAFGRATALLP
                     AVGAEQERAEVLGGHASALWTAGDFAAAQSTAERALELARQMGLAVEEARALATLGFS
                     LAYREDPEAGENALRESLAVAERAAEPREIARAYRSLAELLSGPLNELDRGIEIARAG
                     IERVRALGMVRSAGVGLISVAANGLFRRGRWDEADSAIAEAWELAPSGAEALELRLAR
                     ARMDIGRGRLAPAEDDLEAVEVLSASTVGPRYRIPLLTLRAGLAMWQDRPDVALDHVG
                     FGLDVVEQGSDDVWLVAPLVWHGARARAELARLGLRRPDDGTDARLRRHVADLTRTAA
                     SSVPAIRDSVMGFVEMCKAEDSRAQGHSDPQAWERVAEFWQARQQPYPTAYAHLRRAE
                     ALLALRAHSAAATEALRQAERLARGLGATPMLAEVADLAERGRVKIDVPAPVPHAVPT
                     PEAMTGPARPEPADELTALTARELEVLTELANGHTNREIAQRLFISEKTVGVHVSRIY
                     AKIGVHSRVQASAVLFRSTHGRRGKHTSGPVP"
     gene            14940..15734
                     /locus_tag="VFE39_16615"
     CDS             14940..15734
                     /locus_tag="VFE39_16615"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HET6260261.1"
                     /translation="MAIGDMERQASDPGTTQHLARLAGTTVPASIPEVVRRLAAIHEY
                     ASGTSMLRDGDGIASFTRLYHVITTRIGQMVDSGEFKAADFLIRLDIEFAERYLCALR
                     TYAEDMNRTPGVWRLLFDNRSDPRIAPVNFAIAGVNAHINFDLACALVSTWKHVAPDG
                     DGPESAQFHDYRLINDVFEREMDPLREDLGSLLSRGPDDAIWDRTANWMADLVIRFTR
                     DLAWDEAKRVWERGADDKVMQASAEKLDAIAAFVGERLLRAPVLPV"
BASE COUNT         2247 a         5890 c         5681 g         2079 t
ORIGIN      
        1 gtcggggcgc gggcggtcgc cggtgagcgc gcgcagcacg acgatgtaga gcggtaccgc
       61 ggcgatgagc agggccgcca ggccggacgc caccttctgc tgcgcgatcg tcaccaggcc
      121 gtttccccac gcgggcagca gcagaccgga cagcgccgtc gtaccgagct gctgccacgt
      181 catccggaac gccttccgcc cggcgacggc gagcaccacc aggccgagca gcgatccccc
      241 cacgaggaac cgcgcgccgc tggcgaacag cggcgggacg tcggcgagga tcagccggat
      301 gccgaggtac gtggagcccc agaggacgta gacgacgccg agcgccaccc aggcgatggc
      361 agcggtcggg cggggcgaga cagcggcgtc caccgccctg acgttgccat agccaacaac
      421 tggtgtcgcc ccgaaaacgg gacgaccgcg cacacactgt cgctaccgcg catacgtcct
      481 ccggtgtgca cggtgtgcac ggtggccgtg gccccgaacc gccggcaccg cggccgtcgg
      541 cttcaattgc ccgcatggct accgccaccg tccgtcccgc cgcagtctcc gacgtcgagg
      601 agatcgtgcg catccaggcc tgcacgtggg aggcggcgta cgccgagctc gtccctgccg
      661 cggcgctcgc gcagctgcgg agcgaggcgg cataccacgc ctggacggcc gccgtcgggg
      721 cgggcgccgg ccacgaggtg ctcgtcgcca ccgagggcca gtggacggtc gggttctgcg
      781 ccgccgccgt cgccgcacca ccggagggct ccacggcacc gacggagccg acgctggggc
      841 cgggagcctg ggccgaggtc agcgcgctgc tcgtggaacc gcgctggggc cggcgcggcc
      901 acggcgggcg gctgctcgcc accgccgctg aggcactgcg cggaaccgga gcggtctacg
      961 ggctggcgtg ggttccggag tccgacgagg cgtcccgccg gttctacgcc cgggcgggct
     1021 gggagcccga cggcacgctc cgcgtgctcg acaccggtga cggcgagctg cgcgaggtac
     1081 ggatcaccgg ctccctggat ctgaacctcc agccgtagca ccgccggccg gtctcggtga
     1141 accgtcagtg gtgaggtgcg gggcggcgct ggtcaggcca ggtcgagcag cgcgtcgagg
     1201 ccgacggtca ggccggggcg ctcgcgaacg gcgcgcaccg ccatcagcac accgggcatg
     1261 aacgacgccc ggtcgaacga gtcgtggcgg atggtcagcg tctcccccgt cgtgcccagg
     1321 acgacctcct ggtgggccac caggcccggg agccgcaccg agtgcacgcg gatgccgccg
     1381 atgtccgcgc cacgggcacc gggcagctcg ctcgtggtgg cgtccgggag cgggccggca
     1441 cctgcctcgg cccgcgcctc ggcgatcagc gccgccgtgt gcaccgccgt gcccgacggg
     1501 gcgtcgacct tgcccgcgtg gtgcagctcg atcacctcta cggactcgaa gaaccgcgcg
     1561 gcctggcggg cgaactgcat cgacagcacc gcgccgatgc cgaagttggg ggccacgagc
     1621 acatgtccgg gacgctccgc gagccgcccg cgcaccgcgt cgagcttcgc ctcgtcgaac
     1681 ccgctggtgc cgacgaccgc cgcgatgtcg tgctcgatgc agaaccgcac gttgtcgaga
     1741 gccacgccgg gatgagtgaa gtcgacggcc acctgtgcac cggcgtccac cagcccggtg
     1801 agcgcgtcgc cctcgtcgat ctgcgcgacg agcaccaggt ccggcgccgc ctcgacggcc
     1861 gcgcacacct gcgcacccat gcgcccccgc gcgcccagca cgcccacccg gatcccgttc
     1921 tcagtcacgc ccgcagacta atcagcgcct gcgcgttaac cgacgacgag ccctccgatc
     1981 cacaccgccg cggcgagcaa cccggtggcc agctcgatga cgatgctcca gcccaccgcc
     2041 gcgagcgcct gccgggtgga cagccaggcc tgcccgctcc cccggagacg taccagctcg
     2101 gcgaggtaga tcccgagcac gaaacccagg aacaacccga cgaccgggat cacgaagaac
     2161 ccgaccacgc ccaataccgc ccccgcggcg accgtgcggc cgggcacgcc ggagtcacgt
     2221 agccgccggc cgggcaagag gtatttcgcg acgctgccga gcacgacgat cgccgtcgcg
     2281 atcccgagca cggtccagcc gagggcgtcc ccgcgcggga tcgcccacac ggcgaccccg
     2341 gcgagcacca ggatcagtcc gggcagcacc ggcaccacga tcccgacgat gccgacgacg
     2401 atcagaagcg cggcgatcac ggtctccggg gacaccggct gatcctggca cgcgcggcgc
     2461 cggatcggac gaacggcact ctcgtcccac gatgctggac gaacgggccg ctcggccggg
     2521 cgttacgtca gggaacggag ggacgccggg aggtcccgct ccttgtcgta ggggcccacc
     2581 accgcggccg tgagaggcgc ggagagcagc tcgcgcgcca ggtcggccac ctgctcctgt
     2641 gtgacggccg agatccggtc gaggctctcg acgatcgtgc gctgccgccc gtggtcgagc
     2701 tcgttgcggc cgagccggtt catccgggac ggagtgtcct ccaacccgag caccagcccg
     2761 ccacggaggt tgccctgtgc gcgggcgacc tcgctggcgg tcaacccgtt caccgccacc
     2821 tcggcgagca ccccgcgcac caccgtgacg acctcgtcga gacgctctgg cgcgcatccg
     2881 gcgtagacgg ctacgtggcc cgtgtcggag tagctggtga ccgccgagta gacggagtag
     2941 gccagcccgc gctgctcgcg gacctgctgg aacagccgcg agctcggtcc accgccgagc
     3001 gcggtgttga gcacggtcag taccgtgcgc cgcggatcgt ggcgcgacag gcccggcgcg
     3061 ccgagcatga tgtgcacctg ctcggtgtcc tccggccgga gcaccaagcg cggaccggcc
     3121 gtcagcgcgg cccgctcgag gcggcggggc cccacgggcg gagccgaacc ggccctcgcc
     3181 gccgcgccac ccagcgccgc cgccaccagc tcgacgacct cggagtggac caggttgccc
     3241 gccgcagcga cgaccattcg cggggtggtg tactccccgc gccagaagtc gtggagggtc
     3301 tcgcggctca tcccccggac ggacgcctcg gagccgatca ccggccggcc cagcgggtgc
     3361 tcgccgaaca gagcctcgtc gaacagctcg cccagcaggt cctcgggatc gtcgtcacgc
     3421 atcgcgatct cctcgagcac cactccccgc tcgacctcga cgtcggccgg ggccatcgtc
     3481 gcgtcggtga cgacgtcggc gaggacgtcg accgcgagcg gcagatcggt gtcgagcacg
     3541 tgcgcgtagt agcaggtgtg ctccttctca gtgaacgcgt tgagctcgcc gccgacggcg
     3601 tcgagctgct ccgcgatgtc caccgccgta cgccgccgcg tgcccttgaa cagcaggtgc
     3661 tcgaggtagt gcgcggcacc ggcctgcaac ggcgtctcgt cgcgggagcc gatgccgatc
     3721 cagatcccga gcgacaccga gcggacgccc ggcacggtct cggtaagcac ccgcaggccc
     3781 ccgggcaaca cggtgcggga gacgccgggg gcaggggcga gcgcccccgc ccccggcgtc
     3841 acgagaacgt cggtcaactg ggcgagacct cagctgccgc ggcgtcgatg tcggcagcgt
     3901 cgcctgtgtc gacgccctcg gtggcgggcg cggtgccgtt ctcggactcc tcgcccaccg
     3961 ggatcaggct gatcttgccg cggttgtcga tgtcggtgac ctcgacgcgg atcttgtcgc
     4021 cgaccttgac gacgtcctcc accttggcga tccgcttgcc ctggcccagc ttcgagatgt
     4081 gcacgaggcc gtccttgccc ggcaccagcg acacgaaggc accgaacgcg gcggtcttga
     4141 ccaccgtgcc gaggaaccgc tcaccgatct tcggcagctg cgggttggcg atcgcgttga
     4201 tcatgccgat cgccgcctcg gccgacgggc cgtccgccgc accgacgtag atcgtgccat
     4261 cgtcctcgat ggagatgtcg gcaccggtct gctcggtgat cgagttgatc atcttgccct
     4321 tcgggccgat gacctcgccg atcttgtcga ccgggacctt gatggcggtg acccggggcg
     4381 cgaactggct catctcgtcg gggcggtcga tcgcctcgcc gatgacctcg aggatcgtga
     4441 gccgggcgtc cttggcctgc gacagggccg cggccagcac ctcggacggg atgccgtcga
     4501 gcttcgtgtc gagctgcagc gccgtgacga actcggacgt gccggcgacc ttgaagtcca
     4561 tgtcgccgaa cgcgtcctcg gcgccgagga tgtcggtgag cgcgacgtag cgggtctggc
     4621 cctccacctc gtcgatcacc aggcccatcg cgatgcccgc caccggcgcc ttgagcggca
     4681 caccggcgtt gtacagcgcc atcgtggacg cgcagaccga gcccatcgac gtggacccgt
     4741 tggagctcag cgcctccgac acctgccgga tggcgtaggg gaactcctcg cgcgtgggca
     4801 gcacgggcag cagcgcccgc tccgccagcg cgccgtggcc gatctcgcgg cgcttcggcg
     4861 agcccacccg gccggtctca ccggtcgagt acggcgggaa gttgtagtgg tggaggtagc
     4921 gcttgtgcgt ctccgggccg agcgagtcga tctgctgctc catgcggagc atgttgagcg
     4981 tggtgacacc catgatctgg gtctcgccgc gctcgaacag cgccgagccg tgggcgcgcg
     5041 ggaccacctc gacctcggcc gacagcggcc ggatgtcggt gatgccgcgg ccgtcgatgc
     5101 ggatctggtc gcgcaggatg cgctggcgca ccagcttctt gttcagcgcg cggaacgccg
     5161 cgccgatctc cttctcgcga ccctcgaact gcgggccgac cttgtcgagc accgcggcct
     5221 tgacctcgtc gaggcgcgac tcgcgctcct gcttgccggc gatagcgagc gcctgggcca
     5281 ggtcgtccga cgcgttcgcg gccacggcct cgaacacgtc gtcctggtag gcggggtagg
     5341 tcgggaagtt gcccgtgggc ttggcggcga cgtcggccaa ctgctgctgc gccacgcaca
     5401 ggctgcggat gaacggcttc gccgcctcca gcccggacgc gaccacgtcc tcggtgggcg
     5461 cctgcgcgcc cccggcgacg agctcgatcg tctcggtggt ggcctcggcc tccaccatca
     5521 tgatcgcaac gtcgtcaccg acgattcggc cggcaacgac catgtcgaac actgcccgct
     5581 gcaggtcctc gtactgcggg aaggccaccc actggccgtc gatcagggcg acgcggacgc
     5641 cgccgatcgg gccggagaac ggcagtccgg cgagctgcgt ggacgccgac gcggcattga
     5701 tcgccagcgc gtcgtagggg tcctgcgggt cgagcgacag caccgtgacc acgatctgga
     5761 tctcgttgcg cagaccgtcg acgaacgacg ggcgcagcgg ccggtcgatg agccggcagg
     5821 tcaggatggc gtcggtgcca gggcgaccct cgcggcggaa gaacgagccg gggatcttgc
     5881 cgatggcgta catccgctcc tcgacgtcca ccgtgagggg gaagaagtcg aagtgctcct
     5941 tgggctgctt gctcgcggtg gtggcggaca gcagcatggt ctcgtcgtcg aggtaggcga
     6001 cgacggagcc ggctgcctga cgcgcgaggc gcccggtctc gaaccggatg gtgcgggagc
     6061 cgaagctccc gttgtcgatg acagctgtgg tttcgtgcac gttctcagcc ggatcggttg
     6121 tctgagcatc ggtcatgaac tggacatctc ctctttcaga gggagacccc acgcccacac
     6181 cgggacgatc ctcggccgag ccggtcttcg atcgaagccc ccgggacgtc aatgtgttga
     6241 cccgggagcc actaccgagg accggcggat catctaccgg aggcgtggcg tcgtggagtt
     6301 gtggtgccgg cccggacctg cggagcgatc accgcatggt ccgggccggc gggtggtcag
     6361 cggcggaggc cgaggcgctc gatcagcgac cggtagcgcg cgacgtcgac cgaagccagg
     6421 tacttcagca gccggcggcg acggccgacc agcagcagga gaccccgccg ggagtggtgg
     6481 tcgtgcttgt ggatcttgag gtgctcggtc aggtcgctga tccgcttggt cagcagcgca
     6541 acctgcgcct cgggggaacc ggtgtcaccc tcgtggaccc cgtaggtgtc caggatcgtc
     6601 ttcttctgtg cggcgtcgag tgccacgtgc tcctctttcg ttgatgtgcc gtgaggccgg
     6661 cgccaagaat gtgagacgcc gggagacggt cacggccacc acggactgca gccgaaccca
     6721 gcactccacg ttaccaggca cgatagtgcg cccgtcagcg ccgcctccct agcctccgac
     6781 gccgccgggt gtggccggac ggggcgcgct actcagccag tcggacccgg tggatcggac
     6841 agtagcggga gccgcccctg gcgggctcac tggccaccag cagcacctcc tccggatgcc
     6901 accacgggcc gctgtgcgac gcccaccggt gcaggtgggt cgggatcggc gtcctggccg
     6961 ggcctgcggc gcttccgcag gaccgggagg tgcgggacga acgcggccgg gtcgcccccc
     7021 gccgcggtca caggggcggc cagccggccc gacacatcgg cttccacccc ggcccaccgg
     7081 acgcccggga acgcacccgc accggcgatc cgcagccgcg gccccggcac ccctcccacg
     7141 gcgcggtcga gtctcctggc gaacacccct gggtcggcct caccgtggaa cgccagcgtg
     7201 aggtgccagg tggccgggtc gacggcctgc cacccggatg gcgacccggc cggcgccgtg
     7261 acggccgcga gggcggcgac cgcctccgca ggcggccaca gcgcggtgaa caggcgcatc
     7321 gatcaggccc ccggacggct cgcggcctgg cgcagcacct cggcgagctc cgggaatccc
     7381 gggccgagcg cggctgctgc ggcagccagc cgggccgggt cggcaatccc gcggagcact
     7441 gcggcagccg tgccggagcc gggcccctgg ggatcgcgcg gcgccgggac gcgcggcgcg
     7501 ttccactgcc accgagccct cgcgacctcg gcgagcaggg cgtcgaccgc gcgcggctcc
     7561 ggccggatgc gcaccccgtg gcagcgctgg tcgagcatca gcaaccgcgc gagcaccgtg
     7621 gggtcgccga tcttgacgcg gcgcgcgctc accaactggc tgagcatcgc cgggctgatc
     7681 cccagcgtcc gggcgaggcg cgactgcgag atgcccagtg ccgaggtgag ccggcgaacg
     7741 cggtcgccga gtggcgcgcc gtagagctca cgctgccgat cgcggttcac cgccatccgg
     7801 aaaccgagcg ggcctgcacc tgcgtcagcc atggcaggga tgctgacacc tcgagcaccg
     7861 cttccgcggc cgaacgccga aactgcccgg gctgccgtca gccgcccagc agctcacggg
     7921 ttcgctcgac gtcgcgccgc atctgggcga ccagcgcctc tacgccgtcg aagcgctcct
     7981 gcccgcgcag ccggtgctcg aagtcgagcg cgacctcgaa cccgtagtag tcctcgtcga
     8041 cgtcgagcac gtaggcctcg accgtgcgcg cccgtcccga gaacgtggga ttggtgccca
     8101 ccgagatggc cgccgggagc agcctgctcc cgccgtcgac ggtggtcatc gcgaaccggc
     8161 ccgcgtagac gccgtcggcc ggcagggcgg tgtagggcgg ggtggacagg ttggccgtcg
     8221 ggaagcccag ctcgcggccg cgccggtcgc cgtgcaccac cacgccctcg atccggtgcg
     8281 ggcggcccag cgcggccgtc gccgcctcga cgtcacccgc gtcgatgcag gccctgatgt
     8341 aggtggagga gaacgtgatg ccgtcgtcgg tgatcagttc gagcccctcg acgccgaacc
     8401 cgaaccgctg ccccagctgc gcgagcagcg ccacgtcacc cgccgcgcca cgcccgaagg
     8461 tgaagttgca cccgaccacc acgtccgccg cgtgcagccg ctccacgagc acctcgtgcg
     8521 cgaattcggc gggttcggtg cgggccagtt cggtggtgaa gggcagcacg cagaacgcgt
     8581 ccacaccgag cccggcgaca aggtcggcgc ggcggcggag gctcgtcaac cgggccgggt
     8641 gactgcccgg gcggatcagc tcggcggggt gcgggtcgaa cgtcaccagc acggtgggga
     8701 gcccccgttc ccgcccgcgc tcgaccgcgc gcccgatgag ctgctggtgg ccgcggtgca
     8761 ccccgtcgaa cacgccgacg gtgacgacgc tgcgacccca gccggtcggc acggcctcga
     8821 gcccacgcca gcgctgcacg ggccgagcct acggaaccgc cccaccaccc ccgcgagtcg
     8881 cccgtttccc gcccgcgagt cgccccatcc ggtcccgcga gtcgcccgtt ccctgggcac
     8941 cgccgctggc ccggcggaac cgtcccgacg tggggcagga agaatcgccc cgcgtgaacg
     9001 ccgtctcctc cggcctggtc gtggtcgaca agcccacggg cctgacgtcg cacgacgtcg
     9061 tcgcccggct gcgccggatc ctgagcaccc gcaagatcgg ccacgcgggg acgctcgacc
     9121 cgatggccac cggcgtgctc gtctgcgggg tcggacgtgc aaccaagctg ctcgggcacc
     9181 tcgcgctcga caccaaggcg tacgcggcga ctgtgcgcct cggcgtggtg acgagcacgg
     9241 acgacgccca gggcgaggtc gtcggcacga cggacgccac gggcatcgac gatgcggcga
     9301 tcgccgtcgg gatcgccgct ctgacgggcg cgatcgagca ggtgccgagc tcggtcagcg
     9361 cgatcaaggt cgacgggaag cgggcctacg cgcgggtgcg ggcaggcgag gaggtcgtgc
     9421 tggcggcgcg accggtgacg gtgtcggagt tccggctcct cgcccgacgc ggcggcgacc
     9481 tcgacgtcat ggtggagtgc tcctcgggca cctacgtgcg agccctcgcc cgcgacctcg
     9541 gctccgcgct gggcgtcggc gggcacctga cggcacttcg ccgcacgcgc gtcgggccgt
     9601 tcacgctgga gaacgcccgc accctcgagg aactcgaacg cgagccccgg ctgacgctgc
     9661 cgctggacga cgccgtccgc gtggccttcc cgcgccgtga cctcgacgcc gcactcgcgg
     9721 ctgacatctc gcacggccgc ccgctccccg ctgcgggggt ggagggcacc tacggggtct
     9781 tcgccccgga tggccgggcg ctggcgctgg tcagcgaacg cggcgagctg gcccgcccgg
     9841 tcgtcgtgct cgcccccgcg tagggcgctc ggcgggcgga gaacgggacg actcgcgcac
     9901 ccgaaacggg cgactcgcgg gcccgaaacg ggcgactcgc gggccgaaat cgcgcgactc
     9961 gcgcgcggaa tcgggcgact cgcgggccgg aaacgggcga ctcgcgggcg ttccccggtc
    10021 ctggtgccgc gtcagccggg gcctgcgagc agtccccgcg cgacgaggcc ggcccgccag
    10081 tccatcgccg cccgctccag ctcggcttcg gacggtcgtc cgcgcagcgc ccacaggtgc
    10141 tcggcctgcc gccaggcgtc gtggtgggag aggaggaggc cgaggccggc atcgtcggcc
    10201 gcggcgcccg ggtctcccgc tcctgcggcc tccagtcggt gggcgatcag cccgatgatc
    10261 gtctgcacgt cagcgcgttc ggcaggaccg ggcgttcgtc cgagcagggt gcagatgctc
    10321 acgacggcag gcgcgaggtc gagcccgacc agcgtgagcc cgccggccag ggcgatccgt
    10381 ccggggtcgg ccgtcgccgg atcccaccgg tcgatcacct cctgccatgc cctgggcacg
    10441 ggtgtcccgc gagcgtggcc ccggacggcg cgcaggtagc gcttcgccaa ctccgtgacc
    10501 atcccggcag cgaactcccg gtcggcgccg acgtcgtcga ccgcggcacc gaccaccgca
    10561 ctgagcagcc ggttgaccgg acccacgccc tctccgcgcc gaccggcgtc cgcaccggct
    10621 tgcagccggg ccagcacgtc gacgattcct tgcggcgtga gcggttcgac ggctcccgcc
    10681 gggcccggta cctccggtcc cctcggcccg atcccagcca gtgctctcac cgccggtccg
    10741 gctccggtcg gcccagcagc cggccccagc ggccccgacc gcgccgacgg tctggatgga
    10801 cccagcgccg ccgcgatcac cctcgacccg atcacctcgg ccgtcgtggt gcccattcgc
    10861 atcgctccca tcatcgcccg gtcggtcatc tccgccacga gtcctcctcg gtgaggccgt
    10921 cggccgcgca gcgccggtag cgcacggcga gcgtcgaacc gcggaagagt cgcgtggtgg
    10981 ggccggtgat acgggtggag cgccacagcc accgatatag ggggtccggt agggcggacg
    11041 tagggcgttg tcctgattac acgcgcgcct gctgcgccga ggctggcttc tcaaccggcg
    11101 cacgaccgaa tcccgcggcc gtgacgctca tgacgaagga gcctggcgat gaccgtgtcc
    11161 cccgcgccga cgacgatctc gacactggga aacgccgaac tggtcgagtt ggccgcgcgg
    11221 ggcgacaaca gggcgtgggc ggagatcgtg ctccgttacc ggggtctggt gaccgcggtc
    11281 gtccgctcct tccggctgca ggatgccgac gcccgcgacg ccgagcagcg cacgtggctt
    11341 cggctggtgg aacaccttcg ctcgctgcgg gaccccgacc ggctgggcgg gtggctgtcg
    11401 accactgcat cgcgtgagtg cctgcggatc ctgcgggagg ctcagggcgt cgtgaccgtc
    11461 gagatggacg cggtccccga cccggagtcg ggcgtggagg aacgggtggt cgacgcggac
    11521 actgcggccc gcctgtggcg gatcgtggcc gcgctgccac cgcgtggtcg gacgatcatg
    11581 aacgcgctgt tcgccgagga gccgcggccc tacgcggaag ttgcgcagag cacggggatc
    11641 ccgatcggca gtctcgggcc gagccgggcc cgcctcatcg accgggtccg gcgcagcttc
    11701 gacgagggcg ccttcgcaac cggcgcctga cacggaggag tcgccgtgct atggaacggg
    11761 tccgctcgtg tgcttgcccc ggcggccgtg ggtcgaccgg aacagcaccg cgctggcctg
    11821 tacccggctg tggacgccga tcttcgcgta gatgcggctg acgtgaacgc cgacggtctt
    11881 ctcgctgatg aacagccgct gggcgatctc ccggttcgtg tgaccgttgg ccagctcggt
    11941 gaggacctcc agctcccggg ccgtcaacgc ggtcagttcg tcagcgggct ccggccgggc
    12001 cgggccggtc atcgcctcgg gggtcggtac cgcgtgcggc accggggccg gcacgtcgat
    12061 cttgacgcgg ccacgctctg ccaggtcggc gacctccgcg agcatcggtg tcgcacccag
    12121 cccccgggcg aggcgctcgg cctgccgcag cgcctcggtg gccgcggccg agtgcgcgcg
    12181 gagcgcgagg agcgcctcgg cacggcgcag gtgggcatag gccgtggggt acggctgctg
    12241 gcgggcctgc cagaactcgg ccacccgctc ccacgcctgg ggatcggagt ggccctgcgc
    12301 ccggctgtcc tcggccttgc acatctcgac gaagcccatc acgctgtcgc ggatcgcggg
    12361 gaccgacgag gctgcagtgc gggtcaggtc ggcgacgtgc cggcgcaacc gcgcgtcggt
    12421 gccgtcgtcc ggcctgcgga gccccagccg ggcgagctcg gcacgcgccc tggcgccgtg
    12481 ccagaccagc ggcgccacga gccacacgtc gtccgaaccc tgctcgacca cgtccaggcc
    12541 gaaaccgacg tggtccaacg ccacgtccgg acggtcctgc cacatggcga gaccggcgcg
    12601 gagggtgagc agcgggatgc ggtagcgggg cccgacagtg cttgccgaca gcacctcgac
    12661 ggcctcgagg tcgtcctccg ccggggcgag gcgacccctg ccgatgtcca tacgtgcccg
    12721 ggccagccgc agctcgagcg cctccgcgcc gctgggcgcc agctcccacg cctcggcgat
    12781 ggccgagtcc gcctcgtccc agcggccccg tcggaagagg ccgttggccg cgacggagat
    12841 cagcccgact cccgcactgc gaaccatccc cagggcgcgg acgcgctcga tccccgcgcg
    12901 cgcgatctcg atgcccctgt ccagctcgtt caacggcccc gagagcagct cggcgaggct
    12961 gcggtaggcg cgggcgatct cgcggggctc ggccgcccgc tccgccaccg cgagcgactc
    13021 gcgcagcgcg ttctccccgg cctccgggtc ctcccggtag gccaggctga aaccgagcgt
    13081 cgcgagcgcc ctggcctcct cgaccgcgag ccccatctgg cgcgccagct cgagcgcgcg
    13141 ctcggcggtc gactgcgcgg ccgcaaagtc acccgccgtc cacaacgccg acgcatgccc
    13201 tccgaggacc tcggcccgct cctgctccgc tccgaccgcg ggcagcagcg ccgtggcacg
    13261 tccgaacgcg agggcggcct cagcaccacg gcccgccgcc acgaggtact ggccggtgcg
    13321 ggcgtgcagc agcgcctcgt cgagatcacc gggctcgtcg ggcgtctccg ccatgcggag
    13381 ccgctccccg agcagcgcaa cggcctggtc ggcgtccccg gcgaggtgcg ccgcctcggc
    13441 ggcccgctcc agacacccgg agcgcgggac gggtgcggcg gacagctgcc cggcaagccg
    13501 cgccgcccgc agccaatggc ggtgcgcctc ggcgtaaccg cgcactcggc ctgccgcttc
    13561 ggccgccgcg accgtggccg ccgccgcgcg ctgcacgtca cccgccgact gccagtggtg
    13621 ggcgagccgg gcgtcgacgc cgggcagctc gcgcgtccag gcggcttcga gcgccatcgc
    13681 gtagcgccgg tgcaggtgca cgcgctcacc gggcagcagc tcccggatca cgacgtcggt
    13741 catcaggccg tgccgcagcc ggtaggcgtc cactccgtcg tcgaccacga cgacgcccgc
    13801 gttgaccgcc tcgcgcaagg cgtcgaacag gtcgggcccg ccgccgccca gcacggcctc
    13861 gaggaggtgg tgcggcagcg gaccgtcgca cagggcgatg gcccgcacgg cacgcagcgc
    13921 cggctcggac aggcggttga cgcgggacag cacgagctcg cgcagcgtcg tcggcaggcc
    13981 ggtcggatcc ccgtcgaggg ccgcccgcac ggtctcctcc gcgatgaacg cgttgccggc
    14041 cgagcggttc cacaccagat ccaccaggtc cgtccggccc ggggcgaggg cgcgcaccat
    14101 ctccgccacc gcgtcccggc gcagaggcat gatctccatg gcccgcacct ggcggtgccg
    14161 ccggagctcg ctgatcatcg tgcgcgccgg ggaggcgtcg gtgggcagct cggtgcggtg
    14221 ggtcgcgacg acgagcacgc gctcgtcggc gaggttcgcg accaggtagg cgagcagatc
    14281 gagggtggac cggtcggccc actgcaggtc ctccacgacg aggacgacga cggagccccc
    14341 ggccaggcgc aggaccacgc ggtgcaggag ttcgagggtc tgcacccgcg cctgcgggcc
    14401 cgccagcgcc gggaccgccg ggcggagcgc gaccagctcc tccagctggt ccgcccatgg
    14461 cgacagctgc cgctgcacct cctcaccccg aggcgagcgc aggagggtgc gcagtgccga
    14521 cgcgaccggc cagaacggcg ggctctccgc gatgtcggtg gcgccgccga ggagcacggt
    14581 ggcgttcgca cggtcggcgc ggctcaccag atcctgaacc agccgcgtct tgccgacacc
    14641 cgcgtcgccg accaggaaga ccgtagccat ccggccctgc tgcacctcgt cgagcgcagc
    14701 cgtgagcgca gcgacgtcgt cctccctgcc gacgagcgga ggcgacgcca atcgctcggt
    14761 cacgttcggc gacgttagcc atgcccggga ccggaagcca gaggcacggg cattcgcttc
    14821 acatggtgga gctgttcgcc ccaggaccag ccgccctggg tcggtatcag gacgacaggt
    14881 gaaacctcct cctgccggag ctgtcagcgg gacgggtccg tcgaacgaaa ggagtcgcag
    14941 tggccatcgg ggacatggag cgacaggcaa gcgatcctgg gacgacgcag cacctggcaa
    15001 ggctcgccgg gaccaccgtg ccggcatcga tccccgaggt cgtacggcgg ctcgccgcca
    15061 tccacgagta cgcgtcgggc acctcgatgc tccgcgacgg cgacgggatc gcgtcgttca
    15121 cccggctcta ccacgtcatc accacgcgga tcgggcagat ggtcgacagc ggggagttca
    15181 aggcagcgga tttcctgatc cggctcgaca tcgagttcgc cgagcgctac ctgtgcgcgc
    15241 tgcgcaccta cgccgaggac atgaaccgca cgcccggcgt ctggcgtctg ctgttcgaca
    15301 accgcagcga cccgcgcatc gccccggtga acttcgcgat cgccggcgtg aacgcccaca
    15361 tcaacttcga tctcgcgtgc gcgctggtgt ccacctggaa gcacgtcgcc cccgacggcg
    15421 acgggcccga gagcgcccag ttccacgact accggctgat caacgatgtc ttcgagcggg
    15481 agatggaccc cctgcgcgag gacctcggct ccctgctcag ccgcgggccg gacgacgcca
    15541 tctgggaccg cacggccaac tggatggccg atctcgtcat ccgcttcacc cgggacctgg
    15601 cgtgggacga ggcgaagcgg gtgtgggagc gcggagcgga cgacaaggtg atgcaggcct
    15661 cggcggagaa gctcgatgcg atcgccgcgt tcgtcggtga gcgcctgctg cgggcaccgg
    15721 ttctccccgt gtagtgcgaa cagcgatatc cactcgacgc ccggtccgcc accgagcacc
    15781 catttccgtt cgtgggacac atcgaggcga tggccgtcgg gcaccagctt cccgacggcc
    15841 ggacgctctt ctccgacgtc tcattccgcg tcggcgacgg tgccaagatc gccctcg
//
LOCUS       DASRSC010000341         8509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4726745,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000341 DASRSC010000000
VERSION     DASRSC010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4726745"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="VFE39_16620"
     CDS             <1..399
                     /locus_tag="VFE39_16620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET6260262.1"
                     /translation="EAARALALDGAQIVVSISAWPLSATDPHPDPAQDRWMRRFDLFD
                     RARALENQIVWLAANQAGTFGSLRFASGAKVVDPGGEVLATTGVTAGTAVAELDVAAV
                     VESARRAMGYLRDLRPDAYGAAAAAHPAAA"
     gene            411..1235
                     /locus_tag="VFE39_16625"
     CDS             411..1235
                     /locus_tag="VFE39_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HET6260263.1"
                     /translation="MTAIRIGAVAAGFGRDLTFGLSRIATLVEHARRDGVQLLVLPDA
                     ALGGYLDDITDPDPTALPPALDPTGPELKTVAALARDMVVCVGFCEADGDLRYNTAAC
                     VTGDGVLGLHRKVHQPPGESRVYAAGSEFRAVDTPVGRLGMLVDYDKTFPESARSLAL
                     DGAQVLACLSAWPTSITNRAPRMADDRQAKLFDLYDRSRAAENHVVLVSSNLTGAVGR
                     MRFLGQAKVVGPDGEILARTWSKAGIAVAEVDVTGSVAAARRVLAHLRERRPDAYR"
     gene            1280..1591
                     /locus_tag="VFE39_16630"
     CDS             1280..1591
                     /locus_tag="VFE39_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6260264.1"
                     /translation="MPFEVLAEPTRRRILDLLRERPRLVGELTAELGLSQPGTSKHLR
                     VLRDAGLVTVRADAQRRWYELDPGPLAELDAWLAPYRWMWADRLDALERHLDADTSEG
                     S"
     gene            1588..2343
                     /locus_tag="VFE39_16635"
     CDS             1588..2343
                     /locus_tag="VFE39_16635"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HET6260265.1"
                     /translation="MSENLDTVDGRSVLTMERRLKHPPERVWRAVTEPERLAEWFPTT
                     VTPELRVGGVVKFGFGDAGTVTDFDPPRLFAFTWSGEHLRWELHPDGPGTRLVLVHTF
                     DDRAGAASFAAGWHTCVVALELVLDGRAGEDPGVDHVALHERFVTGFGLDAGSAETTS
                     DGWCVRFERQLTRPADEVWAALSEQAPTGAVRDGQVLEHDAEAGGRLRWELVEGTGHG
                     ARLMLTHTGTEGEPEPALAAGGARIAKLLARLG"
     gene            complement(2400..2987)
                     /locus_tag="VFE39_16640"
     CDS             complement(2400..2987)
                     /locus_tag="VFE39_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET6260266.1"
                     /translation="MLAIGDVARRVLATPPSCGTSRLVCVDGPSGSGKTSLAERLAAA
                     LGDPPLVHMDDLYPGWDGLADAVPLLHDRIVAPLAAGRPAGYRRYDWDLGAYAEEWDL
                     DVPPLLLVEGVASGSRQVARYATLLVWVEAPRAERFRRGIERDGETYRPHWERWARQE
                     EAHFAVEGTRARADLRVDGAPSASHDGERELVLLP"
     gene            complement(2999..4627)
                     /locus_tag="VFE39_16645"
     CDS             complement(2999..4627)
                     /locus_tag="VFE39_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HET6260267.1"
                     /translation="MLGLMQHRSLALPHVFHRAEQYFGHKTIVTAGAQDESTTTVAEW
                     AQRVRRLATVLDTLDVSADGRVGTFCWNTGRHLELYMAVPCTGRVLHTLNVRLFPDQL
                     GYIVNHAEDEVVFVDRSLLPVLWPLVDKFETVRHFVVIDDGADAPLPDDPRVHDYEEL
                     LAVAAPFEGTFTIEDENTAAAMCYTSGTTGNPKGVVYSHRSAVLHALISLTADAVALS
                     ERDVVLPVVPMFHANAWGLPYGCLLAGTDLVFPGPNMTPQAIVALLERHRVTVTGGVP
                     TIWMGMLPLLPAHDLSALRVILCGGSAAPRALSESYREAIGLPMLHAWGMTETSPIAT
                     MSTLRSHHTALTEAERADARARQGQAVPLVDLRLVDPSTGETKPWDDAATGEIQAAGP
                     WIAAQYYRGEGEGAQFTDDGWLRTGDVATVDSYGSVRLVDRTKDLIKSGGEWIGSVEL
                     ENEIMAHPKVAEAAVIAVPHPKWVERPLACVVVKPGETLTAEEVIAHLEPRVAKWWLP
                     DAVEFIDEVPKTSVGKFSKKTLREKFGGYQVAGH"
     gene            4743..6035
                     /locus_tag="VFE39_16650"
     CDS             4743..6035
                     /locus_tag="VFE39_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HET6260268.1"
                     /translation="MTPTDQTASVCLDVGSTWTKAVLVRPDGTLAGFAEHPTTTGDVL
                     AGMDAAVRAVSAAGPSGSGVQPELLACSSAGGGLRLAVIGSERLATTEAGHRVACSAG
                     AHVVHVHAGPLEPADVRVLRSCRPGVVLLVGGADGDDPAGLLHNAGRLARARIRTPIV
                     LAGNSAAQDDALALLRATGRTVVACDNVLPRRGELAPGPARAALIELYQSHAVGGRGP
                     AVAPRFRRLVRVATSDAVGHAATALARICDARVLVVDVGCATTDVHSADQVDVRRTVE
                     GDLGVRAAAGGVLLEGQAEGIVDPVEADLLGPTVARMASELGLVPRDPGSAAEDRRIA
                     ALATVIAVRRHLRVHHDAGRDVELVVLSGGVFRQRGRSGGLAAVVATLRTDPVLAPAL
                     CEAPVVVDADFAVVPAGLLAARGRTTAAESLLRDHLLG"
     gene            complement(6068..6571)
                     /locus_tag="VFE39_16655"
     CDS             complement(6068..6571)
                     /locus_tag="VFE39_16655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007035313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HET6260269.1"
                     /translation="MNSTTVPTTTVQVHRVHIRATPEAVWEAITSPEWTKRYGYHAPA
                     DYDLRAGGAYRSLPSEAMVAHGVPADGPVIDGEVLEVDPPRRLVQTWRMLFTPELVAE
                     GFTRLTYEIEPEAAGVTMLTVTHDVTGAPITAASVSAPPSDAGGGWSVILSDLKTLLE
                     SGEPLYR"
     gene            complement(6568..6891)
                     /locus_tag="VFE39_16660"
     CDS             complement(6568..6891)
                     /locus_tag="VFE39_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HET6260270.1"
                     /translation="MKDDDTVFHALADASRRELLDRLYARDGQTLRELTAGLEMTRQA
                     VSKHLGVLEAANLVVTRRSGREKLHFLNPVPIRLIHDRWIDKYTEYQVTALVDLKTEL
                     EENRP"
     gene            complement(6925..7434)
                     /locus_tag="VFE39_16665"
     CDS             complement(6925..7434)
                     /locus_tag="VFE39_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260271.1"
                     /translation="MTDAEEALRAALRDPALDGGISATALLVGVRSRQQRLLRRRQHA
                     KTLAAAAAAVLVVGGAAVMTARAPQVAPAQQPAPVAPAPTAPAVPPVSPTPVPTAVPP
                     APLTAVSPLVTLMHTPVAVPTPSRGPNGSATQASTPEPRTGAPTSETRAPALSTRRGA
                     PPAAEPTHP"
     gene            complement(7431..7961)
                     /locus_tag="VFE39_16670"
     CDS             complement(7431..7961)
                     /locus_tag="VFE39_16670"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET6260272.1"
                     /translation="MNIVDDPAAAPSFESFVLANGAELVAFARRLTMDHHRAEDIVQD
                     VLARIGLSWSRMATVDDVRAYVFKAILNDFLSWRRRLSSAERPTADLTALRTGYTTGV
                     EDAVLDRDELQEALALLSPVQRAVLVLRYYHDQPDARIAALLGCREGTVRSHARRALT
                     TLRSSQPHRTSEGTRR"
     gene            complement(7958..>8509)
                     /locus_tag="VFE39_16675"
     CDS             complement(7958..>8509)
                     /locus_tag="VFE39_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class F sortase"
                     /protein_id="HET6260273.1"
                     /translation="GLRTPQRSGATIAPPADSAGGAATVEALAPSTPVSLEIPAIEVH
                     SSLLRLGLNPDGSVEVPPLDSPDAGWYENSPTPGQRGPAVILGHVDSARTGPGVFFDL
                     PAVQPGDGITVGRADGTTAAFRVDRVAEYAKSAFPTAAVYGDIDHAGLRLITCGGAFD
                     RSARSYEDNIVVYASAVSAGQAG"
BASE COUNT         1097 a         3026 c         3157 g         1229 t
ORIGIN      
        1 gaggcggcgc gagcgctcgc gctcgacggc gcccagatcg tcgtctcgat ctcggcctgg
       61 ccgctgagcg ccaccgaccc ccatccggat ccggcccagg accggtggat gcgccgcttc
      121 gacctcttcg accgggccag ggcactggag aaccagatcg tctggctcgc ggcgaaccag
      181 gccggcacgt tcggctcgct ccggttcgcg tccggcgcga aggtcgtcga tcccggcggc
      241 gaggtgctcg ccaccaccgg tgtcacggcg ggtaccgcgg tagcggagct ggacgtcgcg
      301 gccgtggtgg agtcggcccg gcgagccatg ggatacctgc gcgacctgcg gccggatgcg
      361 tacggcgcgg cggcggctgc gcaccccgcg gccgcctgaa caagccgggc gtgacagcga
      421 tccggatcgg agccgtggct gccggcttcg gcagggacct gacgttcggc ctctcccgca
      481 tcgcgacgtt ggtggagcac gcccgccgcg acggcgtgca actgctcgtg ctgcccgacg
      541 ccgcgctcgg cggttacctc gacgacatca ccgatcccga tcccactgcg cttccgcctg
      601 cactcgatcc caccggaccg gagctgaaga ccgtcgcggc gctggccagg gacatggtgg
      661 tgtgcgtcgg attctgcgag gccgacggtg acctgcgtta caacaccgcg gcgtgcgtca
      721 ccggcgacgg agtgctgggc ctgcaccgga aggtgcacca gccgcccggc gagtcgcggg
      781 tctacgcggc aggctcggag ttccgcgccg tggacacgcc ggtcggacgg ctcggcatgc
      841 tcgtcgacta cgacaagacc ttcccggagt cggcccgcag cctcgccctc gacggcgcac
      901 aggtgctcgc ctgcctctcg gcatggccga ccagcatcac gaaccgcgcc ccacggatgg
      961 ccgacgaccg gcaggccaag ctgttcgacc tctacgaccg gtcgcgggcg gcggagaacc
     1021 atgtggtgct cgtctcgtcg aacctgaccg gggcggtcgg ccggatgcgc ttcctcggcc
     1081 aggccaaggt cgtcggccca gacggggaga tcctggcccg cacctggtcg aaggcgggca
     1141 tcgcggtggc cgaggtggac gtcacgggat cggtggcggc ggcgcgccgg gtgctggcgc
     1201 acctgcggga gcgacgaccc gacgcctacc gatgattaat gctcttgcgg ttatatagct
     1261 gtttgagtat gttcgaggcg tgccgttcga ggtgctcgcc gagccgaccc gtagacgcat
     1321 cctcgacctg ctccgggagc gcccgcggct ggtcggggag ctgaccgcgg agctcgggct
     1381 gagccagccc ggcacgtcga agcacctgcg ggtgctccgc gacgccggcc tggtgacggt
     1441 gcgggccgac gcccagcgcc gctggtacga gctcgaccct ggcccgctcg ccgagctgga
     1501 cgcctggctc gcgccgtacc gctggatgtg ggccgaccgg ctcgatgccc tcgaacggca
     1561 cctcgacgcc gacacctcgg agggatcatg agcgagaatc tggacaccgt cgacggccgc
     1621 tccgtgctga cgatggagcg ccggttgaag cacccgccgg agagggtgtg gcgggcggtc
     1681 accgaacccg agcggctcgc ggagtggttc cccaccacgg tgacgccgga gctgcgcgtc
     1741 ggtggcgtcg tcaagttcgg gttcggcgac gccggcaccg tcaccgactt cgacccgccg
     1801 cggctgttcg cgttcacctg gagcggcgag cacctgcgct gggagctcca ccccgacggc
     1861 ccgggcacac ggctcgtgct cgtgcacacg ttcgacgacc gggctggcgc cgccagcttc
     1921 gccgcgggct ggcacacgtg cgtcgtcgcg ctcgagttgg tgctggacgg gcgggcaggc
     1981 gaggacccgg gcgtcgacca cgtggccctg cacgagcggt tcgtcaccgg cttcgggctc
     2041 gacgccggat cggccgagac cacgtccgac ggctggtgtg tccgcttcga acggcagctc
     2101 acccgtccgg ccgacgaggt gtgggcagcc ctgtcggagc aggccccgac aggcgccgtc
     2161 cgggacgggc aggtgctgga gcacgacgcg gaggcgggcg ggaggctgcg ctgggaactg
     2221 gtcgagggca ccggccacgg cgcccgcctg atgctcacgc acaccggcac cgagggcgag
     2281 ccggagccgg ccctggcggc cggcggcgcc cgcatcgcga agctcctggc gcgcctcggg
     2341 tgagcagcct gccggggcgg tcgcgatcga aacgcggctg gtgcgactcg ggtcccgtgt
     2401 caggggagca gtacgagctc cctctccccg tcgtgcgagg cggagggggc tccgtccacg
     2461 cgtaggtcgg cgcgggcccg ggtgccctcg acggcgaagt gcgcctcctc ctgccgggcc
     2521 cagcgctccc agtgcgggcg gtaggtctcg ccgtcgcgct cgataccgcg gcggaaccgt
     2581 tcggcccggg gtgcctcgac ccagacaagc agcgtcgcgt agcgcgcaac ctgccgggac
     2641 cccgacgcca ctccctcgac gagcagcagc ggcggcacgt cgaggtccca ctcctctgca
     2701 taggcgccga ggtcccagtc gtaccggcgg tacccggcgg gtcgtccggc cgcgagcggc
     2761 gcgacgatcc ggtcgtgcag cagcggcacg gcgtcggcga ggccgtccca gccggggtag
     2821 aggtcgtcca tgtgcaccag cggcgggtcg ccgagtgcag cggccagccg ctccgcgagc
     2881 gacgtcttgc ccgaacccga cggaccgtcg acgcacacca gccgcgacgt tccgcacgac
     2941 ggcggcgtgg cgagcacccg gcgagcgacg tcgccgatcg ccagcatgcc gccgcgggtc
     3001 agtgcccggc cacctggtag ccgccgaact tctcgcggag cgtcttcttg gagaacttgc
     3061 cgacgctggt cttgggcacc tcgtcgatga actcgaccgc gtcggggagc caccacttcg
     3121 cgacgcgggg ctccaggtgg gcgatcacct cctccgcggt gagcgtctcg ccgggcttga
     3181 ccaccacgca tgccaggggc cgctccaccc acttcgggtg cggtaccgca ataacggcgg
     3241 cctcggcgac cttggggtgc gccatgatct cgttctccag ctccacggag ccgatccact
     3301 ccccgccgga cttgatcagg tccttggtcc ggtcgacgag gcggacggag ccgtagctgt
     3361 cgaccgtggc gacgtcgccc gtgcggagcc agccgtcgtc ggtgaactgc gcgccctcac
     3421 cctcgccgcg gtagtactgg gccgcgatcc acggcccggc cgcttggatc tccccggtgg
     3481 cggcgtcgtc ccacggcttg gtctcgccgg tcgaggggtc gacgagccgc aggtcgacca
     3541 gcggaacagc ctgcccctgc cgggccctgg cgtcggcccg ctccgcttcg gtgagggcgg
     3601 tgtggtggct gcgcagcgtg gacatcgtgg cgatcgggct ggtctcggtc atcccccagg
     3661 catgcagcat cggcaggccg attgcctccc ggtagctctc ggacaacgcc ctcggcgccg
     3721 ccgagccacc gcagagaatc acccgcagcg ccgacaggtc gtgggccgga agcagcggca
     3781 gcattcccat ccagatggtc gggacgccgc cggtgacggt gacgcggtgc ctctccagca
     3841 gcgccacgat cgcctgcgga gtcatgttcg ggccggggaa caccaagtcc gtccccgcga
     3901 ggaggcagcc gtagggcagg ccccatgcgt tggcgtggaa catcgggacc acgggcagca
     3961 cgacgtcgcg ctcggacagc gccaccgcgt cggcagtcag cgagatcagc gcgtgcagca
     4021 cagcggaccg gtggctgtaa acgacgccct tggggttgcc ggtggtgccc gacgtgtagc
     4081 acatcgcagc cgcggtgttc tcgtcctcga tcgtgaaggt gccctcgaac ggcgccgcca
     4141 cggcgagcag ttcctcgtag tcgtgcaccc gcgggtcgtc gggcagcggc gcgtcggcgc
     4201 cgtcgtcgat caccacgaag tgacgcaccg tctcgaactt gtcgaccagc ggccagagca
     4261 ccggcagcag cgaccggtcg acgaacacga cctcgtcctc cgcgtggttc acgatgtagc
     4321 cgagctggtc gggaaacagc cggacgttca acgtgtgcag cacgcgtccg gtgcacggca
     4381 ccgccatgta cagctcgaga tgtcgacccg tgttccagca gaacgtgccg accctcccgt
     4441 cggcgctcac gtcgagggtg tcgaggacgg tggcgagccg ccgcacccgc tgcgcccact
     4501 cggcgaccgt cgtggtgctc tcgtcctggg cgcctgccgt gacgatcgtc ttgtgcccga
     4561 agtactgctc ggcccggtgg aagacgtgcg gcagggcgag ggagcggtgc tgcatcagtc
     4621 cgagcatctg gcctcctgtg tgctctgtgc tctgagtaac tcgcaagtta ccgccggttc
     4681 ggcccggtga ccatcgcgcc gacacaggcc atcgggcccg tctgccgtta ggctcaggtg
     4741 ccgtgacgcc caccgatcag accgcctcgg tctgcctcga cgtgggttcc acctggacga
     4801 aggccgtact ggtccgtccg gacggcacgt tggcgggctt cgccgagcac ccgacgacca
     4861 caggtgacgt gctggcgggg atggatgccg ccgtccgcgc ggtctcggcc gccggcccgt
     4921 caggctccgg ggttcagcca gagttgcttg cctgctcgtc ggcgggcggc ggcctgcgcc
     4981 tcgcggtgat cggttccgaa cggctggcca cgacggaggc cggccatcgc gtcgcctgct
     5041 cggcgggcgc ccacgtcgtg cacgtacatg ccggtccgct cgaacccgcc gacgtccggg
     5101 tgctgcgcag ctgccggcca ggggtggtgc tgctcgtcgg gggcgcggac ggcgacgacc
     5161 cggccgggtt gctgcacaac gcgggccgcc tggcgcgcgc ccgcatccgc actccgatcg
     5221 tgctcgccgg gaactcggca gcccaggacg acgcgctggc gctgctgcgg gccaccggcc
     5281 gcaccgtcgt ggcgtgcgac aacgtgctcc cacgccgtgg tgagctggcg ccggggccgg
     5341 cgcgggcggc gttgatcgag ctctaccaga gtcatgcggt cggcggccgg ggcccggccg
     5401 tcgcgccccg gttccgcagg ctcgtgcgcg tggcgacctc cgatgccgtc ggccatgccg
     5461 ccacggcgct cgcccggatc tgcgacgctc gggtgcttgt cgtcgacgtc ggttgcgcca
     5521 ccaccgatgt gcactccgct gaccaggtgg acgtccgccg aacggtcgag ggcgacctcg
     5581 gcgtccgcgc cgcggcaggc ggggtgctcc tcgaaggtca ggccgagggc attgtcgacc
     5641 cggtcgaggc ggacctgctc ggcccgaccg tcgcccggat ggcgagcgag ctcggcctcg
     5701 taccgcgcga tccgggcagc gcggccgagg atcgccggat cgcagcgctc gccacggtga
     5761 tcgcggtgcg ccggcacctg cgggtgcacc acgatgcggg acgggacgtg gagctggtgg
     5821 tgctctccgg cggtgtcttc cggcagcggg gccggtcggg tggcctcgcc gccgtggtgg
     5881 cgacgctgcg caccgacccc gtcctcgcgc ccgctctgtg tgaggcgccg gtggtggtgg
     5941 acgccgactt cgccgtggtc ccggccgggt tgctcgccgc aagggggcgc acgacggccg
     6001 ccgagtcctt gctgcgggac cacctgctcg gctgagtccg agcaggtggt ccggcccgcg
     6061 tgcgcggtca gcggtagagc ggctcgccgc tctcgagcag ggtcttgagg tcgctgagga
     6121 tgacgctcca gccaccgccc gcgtccgatg gcggtgcgct gaccgaggcc gcggtgatcg
     6181 gtgcgccggt gacgtcgtgc gtgaccgtca gcatcgtgac gccggccgcc tccggctcga
     6241 tctcgtaggt gagccgggtg aagccctccg cgacgagctc cggggtgaag agcatgcgcc
     6301 acgtctggac cagccggcgg ggcggatcga cttcgaggac ctcgccgtcg atcaccgggc
     6361 cgtccgccgg gacgccgtgc gccaccatcg cctcgctcgg gagggatcgg tacgccccgc
     6421 ccgcgcgcag gtcgtagtcg gcgggggcgt ggtagccgta ccgcttcgtc cactccggac
     6481 tggtaatcgc ctcccagacc gcctcgggcg tcgcccggat gtgcacccgg tgcacctgga
     6541 cggtggtggt gggaacggtg gtgctgttca tggccggttc tcctcgagct cggtcttgag
     6601 gtccacgagc gccgtgacct ggtactcggt gtacttgtcg atccaccggt cgtggatcag
     6661 ccggatcggc accgggttga ggaagtgcag cttctcccgc cccgaccggc gcgtgacgac
     6721 gaggttggcc gcctccagca cgcccaggtg cttgctgacc gcctgccgcg tcatctcgag
     6781 gcctgccgtc agctcccgca gcgtctgacc gtcgcgggcg tacagccggt cgagcagctc
     6841 acgccggctc gcatcggcca gggcgtggaa gacggtgtcg tcgtccttca cgtgggccag
     6901 aataggcaac tagaaggttg cctgtcaagg gtgcgtgggc tccgccgccg gcggtgcgcc
     6961 ccgccgggtc gacagtgccg gtgccctggt ctccgacgtc ggggccccgg tccgcggttc
     7021 gggcgtggat gcctgcgttg cgctgccgtt cgggcccctg ctcggcgtcg gaacggccac
     7081 cggtgtgtgc atcaaggtca ccagcggact cacggcggtc agaggggcgg gcggtacggc
     7141 cgtgggcacg ggggtcggag agaccggtgg cacggcaggc gcggtcggcg cgggtgcgac
     7201 cggcgcgggc tgctgggcag gcgccacctg cggtgctcgc gcggtcatca ccgccgcacc
     7261 gccgacgacc agcacggcag cagcggctgc ggcgagcgtc ttcgcgtgct ggcgcctgcg
     7321 cagcagccgc tgctgccggg accgcacgcc gacgagcagc gcggtcgccg agatgccccc
     7381 gtccagggcg gggtcgcgca gggcggctcg cagcgcttct tccgcgtcgg tcatcgccgg
     7441 gtcccttcgc tcgtgcggtg gggctgcgag ctgcgcaggg tcgtcagggc gcgccgggcg
     7501 tgggagcgca cggtgccttc gcggcacccg agcagcgccg cgatccgggc gtcgggctga
     7561 tcgtggtagt agcgcagcac gaggacggcc cgctgcacgg gcgagagcag tgccagcgcc
     7621 tcctgcagct cgtcgcgatc caggacggca tcctcgacgc cggtcgtgta gccggtgcgc
     7681 agtgcggtga ggtccgcggt ggggcgctcg gcggacgaca gccggcgccg ccacgagagg
     7741 aagtcgttga ggatcgcctt gaagacgtag gcgcggacgt cgtcgaccgt ggccatccgc
     7801 gaccacgaca ggccgatcct ggccaggacg tcctggacga tgtcctcggc ccggtggtgg
     7861 tccatcgtca ggcgccgtgc gaacgccacg agctccgcgc cgttggcgag cacgaacgac
     7921 tcgaacgacg gcgcagccgc cggatcgtcg acgatgttca cccggcctgt cctgcggata
     7981 cggcggaagc gtagacgacg atgttgtcct cgtagctgcg cgcgctccgg tcgaaggcgc
     8041 cgccgcaggt gatcagccgc agcccggcgt gatcgatgtc gccgtacacc gcagctgtgg
     8101 ggaacgcgct cttcgcgtac tcggccacgc ggtcgacgcg gaacgccgca gtggtcccgt
     8161 cggcgcggcc caccgtgatc ccgtcgccgg gctgcacggc tggcaggtcg aagaagaccc
     8221 cgggccccgt gcgcgccgag tcgacatggc cgaggatcac cgcgggtccc cgctggcccg
     8281 gtgtcggcga gttctcgtac cacccggcgt ccggggagtc gagcggtggc acctcgacgc
     8341 tcccgtccgg gttcagcccc agccgcagca gcgacgagtg cacctcgatc gccgggatct
     8401 ccagcgacac cggggtcgag ggagcgagcg cctccaccgt cgccgccccg cccgccgagt
     8461 cggcgggtgg agcgatggtg gcgccggacc gctgcggcgt gcggaggcc
//
LOCUS       DASRSC010000342         9182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5056311,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000342 DASRSC010000000
VERSION     DASRSC010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9182
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5056311"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFE39_16680"
     CDS             <1..243
                     /locus_tag="VFE39_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HET6260274.1"
                     /translation="CPVRTECLAHALDQRIEFGVWGGMTERERRALLRARPDVGSWAE
                     LLADARIAHYSSAAAETADALAMVAEPEAAQLEESA"
     gene            complement(311..1447)
                     /locus_tag="VFE39_16685"
                     /pseudo
     CDS             complement(311..1447)
                     /locus_tag="VFE39_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466701.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
     gene            complement(1444..2472)
                     /locus_tag="VFE39_16690"
     CDS             complement(1444..2472)
                     /locus_tag="VFE39_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HET6260275.1"
                     /translation="MTSSTWPAALSAARLHVVSGKGGTGKTTVAAALALALASGGRRT
                     LLIEVEGRQGIAQLFDTPPLPYEERKIAVAPGGGEVRALAVDTEAALLEYFELFYRLG
                     MAGRTLRRMGAIEFATTLAPGLRDVLLTGKAKECVNRRGPDGRPVYDAVVLDAPPTGR
                     VISFLDVTKAMADLAKSGPIHSQAAGVVQVLHSELTAVHLVTLLEDLPVTETLETVDE
                     LTAAGFPIGAVIVNRVRPQWLPDRSVTAAANGRVDAERIRAGLSAAGLDLPADVIDGL
                     VAETVDHAVRVHAEGIALSRLDDGPLLPRLELPQLVDGVDLGSLYELAETLVEQGVAV
                     HTLSGAAS"
     gene            2546..2725
                     /locus_tag="VFE39_16695"
     CDS             2546..2725
                     /locus_tag="VFE39_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260276.1"
                     /translation="MSAPGPSVTTWEYLTAPVLIHSTQAILNNFGRDGWELVAVTTGA
                     TPEQLVAWFKRPVQG"
     gene            2730..3194
                     /locus_tag="VFE39_16700"
     CDS             2730..3194
                     /locus_tag="VFE39_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009946926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET6260277.1"
                     /translation="MASPSERLRELDIALPSVAAPAGSYIPARRTGSLVFTSGQVPFV
                     DGAPAGIGKVGAEIEPDEAYRLARICALNALAAVDALVGIDAVTGVVKVVGFVASAPG
                     FTGQPGVVNGASDVLGEIFGEAGRHARSAVGVAELPLGVPVEVELVVEVEGP"
     gene            3191..3781
                     /locus_tag="VFE39_16705"
     CDS             3191..3781
                     /locus_tag="VFE39_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012782874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260278.1"
                     /translation="MTAADQACHELLVRLAGRLPDELHWRLRDWLADGGQAALSATLP
                     RELLRHRVGLTDDERELLAAAVGQWGASRRLLDAVLPAHASDEPAVAFAPGPDGLDQA
                     ALSILAVVRGHPGCVALRQAWRSGGRGRPRVVLVLGGERPWVLTGTLQRVLRAHGDRT
                     PCVEVLPPHGEPPSYHRAAIMGSVSLWRSVAALVGT"
     gene            3872..5932
                     /locus_tag="VFE39_16710"
     CDS             3872..5932
                     /locus_tag="VFE39_16710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260279.1"
                     /translation="MEDETSPETTHDLLLSLAGRVDDDLLAWARELAAVGEDARAVEL
                     LTASLVAARTVLPDPVRAGLVAAARSARIDLDPAAALPPSRAEQGSAHRFDAGPPDDV
                     VSAVLLAVPARQVQGCRILVARRLTPAGSAPGPLPHPVVLVEVLPGARATDVLAYQLA
                     ATLERAGVPASVEVLTADAPVPAYHAAALRRARLIRSDDGGERHMAEPEWAPGRPSAV
                     FGAGPASMPGHRHVRHPLAPPQPDVAGLLGTPARPGDLRGDPRPAPAADALPQRTPGP
                     NPEPVDPEAGTLSLPAVPNRGTPLSAVRPEASDPPPVRPDAEPSAASVDPPEGHEPDA
                     PPGPSSPSGPPTGQDPRPRPGPTARTLRPPGTPDARPPLPSPIPLVRRNGPTPTAHLF
                     EPVAEQPGEAAEQLFGEQIQRPAEAVDNPRPPRNTAMDTPLPHRMNGLSRTLGETPAF
                     DSLNDPLSGPLSEPLLAPLLDPTIREDDPLGVDGRMPAAPEPARAEEDWSSEWQSGSW
                     AMSPSALDDDEPEDTSDASPEIAAPDVEERPDARPAPRRAARHRYVDEEGDADGEGRP
                     PEPEAEAPRDRSPEAQPAPEPEPVAEPVAEDAPAAAPEPDGTPDPVPASADLGLRPES
                     LARLSDADRQLLARLQAELLDGRKPRVSRRAGIANGSGTNGSSGNGSGINGAPRADPP
                     DLAG"
     gene            complement(5937..6836)
                     /locus_tag="VFE39_16715"
     CDS             complement(5937..6836)
                     /locus_tag="VFE39_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6260280.1"
                     /translation="MGSVDQLRVGLVGGGPWARTVHGPALAAHPGTAMAAVWTRRPEF
                     AHEVAEEFGARAHARLDDLLDDVDVVAFAVPPQVQGELAVRAAAVGRHLICEKPLAGS
                     VEDARAVVDAVQRAGVHSSMVLTMRHAPAVREWLAALHSAPAGPDTVASARWLSGSLL
                     GGPYSASAWRIEHGALLDLGPHVIDLLDAAVGAVTSVGWAHHDQPDLWRFGLVHEGGA
                     HSTVTLSMRLPIDPSEIEFAVFGPNGRHRLAGRHADPRTCFAALLDELVAAIDGSGPP
                     PALDVARGLRLQELVEQVRSAAG"
     gene            6866..7675
                     /locus_tag="VFE39_16720"
     CDS             6866..7675
                     /locus_tag="VFE39_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6260281.1"
                     /translation="MPSEPVVPRPAATVMLVRDNPDPPPGRTPLQVFLQRRDPGMAFA
                     PRMTVFPGGGVDATDHDVLPSWAGPDPRWWGERLDCDATLAGALVQAAVRETFEECGV
                     LLAAPELPARLAGYRADLVARRLTFAQVLAETGLVLRADLLRAWARWITPPTSPRRYD
                     TVFFVARVPEGQEADAHTTEAVEATWWHPAEAVEHWQRGEIQLMAPTFRTLQEIAEHP
                     DSGAVLAAADDRVVRPVIPRVRREGEQIVVVLPDDPDFHIAVDHLGPGASA"
     gene            7672..8463
                     /locus_tag="VFE39_16725"
     CDS             7672..8463
                     /locus_tag="VFE39_16725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6260282.1"
                     /translation="MSHPLYGKLRPVTPLASVLLAQNPSPMTLEGTNTWVLRAPGVEE
                     CVVVDPGEADEEHLGRVAAHGPVALVLLTHRHHDHAGGARRFAELTGAPVRALDPSLV
                     LGSEALGDGDVVAVAGVEVRVVGTPGHTSDSLSFLLDGPDSETAVLTGDTILGRGTTV
                     IAHPDGALRPYLDSLRRLAELLPGTAVLPGHGPELPDAPAAAAAYLAHREQRLEQVRG
                     ALGRLGPDATARQVVELVYADVDRALWPAAEWSVRAQLDYLRASA"
     gene            complement(8472..>9182)
                     /locus_tag="VFE39_16730"
     CDS             complement(8472..>9182)
                     /locus_tag="VFE39_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6260283.1"
                     /translation="LETSIVLIAVALVGIVTGSVGFAVTHAVRTHHPVPARPAGVFGA
                     LAVPGLRTVVLASLGFGLVIGTVEVGVPAVTAAAGSAALGGVLLSAWSVASVLAGVAY
                     SLRPWPRPLHLRLPVLLLAFAVCVGAMAVAGPLPALVVAMLLAGATITPQVTAHSLGV
                     EATAPAGTATEAFGWVVTAATLGLAAGQSMAGIVVEAAGPPAAFLASGSAGVVLAVVL
                     WLRRGTLAPTTTPAPVAA"
BASE COUNT         1217 a         3491 c         3363 g         1111 t
ORIGIN      
        1 tgtccggtcc gcacggaatg cctggcacat gcccttgacc agcgcatcga gttcggcgtc
       61 tggggcggca tgaccgaacg ggagcggcgc gccctgctgc gcgcccgccc cgacgtgggg
      121 tcgtgggccg agctgctggc cgatgcacgc atcgcgcact attcgtcggc ggcggcggag
      181 acggctgacg cgctcgccat ggtggcggag ccggaggccg cgcagctgga ggagtcggcg
      241 tgagcgttga ccggctaccg ggcggagcgc agggcccgtc ccggtgcggc ggccgcatgc
      301 tcctcgccgt cgcccgccgc gagccgatct cacgcagccc gtcgaggtcg gcgatgtcgt
      361 tcgccaccac cggcaccctg gtcacggcca cggcggggtg ggcgctggtg aaccgggaga
      421 gcagccgctc ctcccgctcg gccaggtcca cccggtcggc atgcagccgc agcaccgcgg
      481 ccgcgagcgg cgcgccgcgc agaccctcgg cggcatcgcg tgccttcgtc gccgacagct
      541 gcgcccgcac gggatgggtg cggttgagca cgagaccggc gagcggcatg tcgtcggcgg
      601 agagccggtc cacgaagtag gcggcctcgc gcagggcatc gggttccggc gcggcgacca
      661 ccaggaacgc ggtgccgggc gagcggagca gcgcatacgt ggcggttgcg cgctcgcgaa
      721 acccgccgaa catcgtgtcg aaggcctgca cgaacgccga agcgtcggcc agcagctgcc
      781 cgcccaggat cgtggagacc gccttggcga acaaggtgaa gccggccccg acgatcttgc
      841 gcagccccct cccgcccgcg cgcgggggcg ccgacaacag ccggatcatc cggccgtcga
      901 ggaagttcga catgcgctgg ggtgcgtcga ggaagtcgag cgccgagcgc gacggcgggg
      961 tgtcgaccac gatcaggtcc cactcgccgg tggccgcgag ctgccccagc ttctccatcg
     1021 ccatgtactc ctgcgtgccc gagaacgacg aggagatggt ctggtagaag ggattctcga
     1081 tgatgctctc ggcgcggccc ggctctgcgt gtgcgtagac catctcgtcg aaggtccggc
     1141 gcatgtccag catcatcgcg tgcagttcgc cgccgcccgg cgcgccgacg cccgactcgt
     1201 tgacgcctgc cacgcggcca ggctcgttgt tgagagccgc caggccgagc gcctgcgcga
     1261 gccgcttggc cggatcgatc gtgaggacca ccgtgcgccg cccgcgctcg gcggccctga
     1321 tcgccagggc ggccgacgtg gtcgtcttgc cgacgccgcc ggagccgcag cagaccacga
     1381 catgggtgtc cgggtcgtcc agcagcgcgt cgatgtccag caccggggca acgcgggcgg
     1441 ggctcacgag gccgcaccgc tcagcgtgtg caccgcgaca ccctgctcga cgagcgtctc
     1501 cgccagctcg tagagggagc cgaggtcgac accgtcgacc agctgcggca gttccagccg
     1561 gggcagcaag ggcccgtcat cgagccggga gagcgcgatg ccctccgcgt gcacgcgtac
     1621 agcgtggtcg acggtctcgg cgaccaggcc gtcgatcacg tcggcaggga gatcgaggcc
     1681 ggccgccgac aaccccgcac ggatccgttc ggcgtcgacc cgtccgttcg cggcggcggt
     1741 gacggagcgg tccggcagcc attgcggccg cacccggttg acgatcacag caccgatcgg
     1801 gaagccggca gcggtcagct cgtcgacggt ctcgagcgtc tcggtcaccg gcaggtcctc
     1861 caacagcgtc acgaggtgga cggcggtcag ttccgagtgc agcacctgca ccacgccggc
     1921 cgcctgactg tgaatcggtc cgctcttcgc cagatcggcc atcgccttgg tgacgtcgag
     1981 gaagctgatg acgcggcccg tcggcggggc gtcgagcacc acggcgtcgt agacggggcg
     2041 cccgtccggc ccgcggcggt tgacgcactc cttcgccttg ccggtgagca gcacgtcccg
     2101 cagcccgggc gccagcgtgg tggcgaactc gatggccccc atccggcgca gcgtccggcc
     2161 tgccatcccg agccggtaga agagctcgaa gtactccagg agcgcggcct cggtgtccac
     2221 cgcgagcgcc cggacctccc cgccaccggg agccacggcg atctttcgct cctcgtaggg
     2281 cagcgggggc gtatcgaaca gctgggcgat gccctgccgg ccctccacct cgatgagcag
     2341 cgtgcgcctg ccccccgagg ccagtgccag cgcgagggca gcggccaccg tggtcttgcc
     2401 cgtgccgccc ttgccggaga cgacatgcag gcgggccgcg gacagcgcgg cgggccacgt
     2461 ggaagacgtc acgtctgtca ggttacggag cttgcgtccg accggccgcc tgaacggtgt
     2521 gaggcgctgg ctacagtgac cgctcatgag cgcccccggt ccatcggtca cgacgtggga
     2581 atacctcacc gctccggtgc tgatccacag cacccaggcg atcctcaaca acttcgggcg
     2641 cgacggctgg gagctggtgg cggtcacgac cggtgccact ccggagcagc tcgtcgcgtg
     2701 gttcaagcgc ccggtacagg gctgacggca tggcgtcccc gagcgagcgg ctccgcgagc
     2761 tggacatcgc actcccgtcc gtcgcggccc ccgcagggtc ctacatcccg gcgcgccgca
     2821 cgggttcact ggtgttcacc tccggtcagg tgccgttcgt cgacggagcg ccggccggta
     2881 tcgggaaggt cggcgccgag atcgagcccg acgaggctta ccggctcgcg cggatctgcg
     2941 ccctgaacgc gctcgccgcg gtggatgccc tcgtgggaat cgacgccgtc accggtgtgg
     3001 tgaaggtggt cggcttcgtc gcctcagctc ctggtttcac cgggcagccc ggcgtcgtca
     3061 acggggcgtc cgacgtgctc ggcgagatct tcggcgaggc gggcaggcac gctcgctccg
     3121 ccgtcggggt ggccgagctg ccgctgggcg tcccggtcga ggtggaactg gtcgtcgagg
     3181 tggagggccc gtgaccgccg ccgaccaggc ctgccacgaa ctcctggtcc ggctggcagg
     3241 caggcttccc gacgaactgc actggcgcct gcgcgactgg ctcgccgacg gcggccaggc
     3301 cgccctcagc gccacgttgc cgcgggaatt gctgcgccat cgcgtcgggc tcaccgacga
     3361 cgagcgggag ctgctcgcgg ccgccgtcgg gcagtggggg gcctcgcgcc ggctgctgga
     3421 cgccgtgctg cctgcgcacg cgagcgacga gccggccgtg gccttcgcgc ctggtcccga
     3481 cgggctggac caggccgcgc tcagcatcct cgccgtggtc cgcggccacc ccggctgtgt
     3541 agcgctgcgg caggcctggc gcagcggggg gcgcggcagg ccacgggtcg tgctcgtgct
     3601 gggcggcgaa cggccgtggg tactcaccgg cacgctgcag cgggtgctac gcgcccacgg
     3661 cgaccgcaca ccgtgcgtgg aggtgctccc gccgcatgga gagccaccgt cctaccaccg
     3721 ggccgccatc atgggatcgg tatcgctgtg gcgctccgtg gcggcgctcg tcgggacgtg
     3781 acgggccgcc gtgacaggcg ggggatgacg gtccgcaggt gtcaggcgcc gcatgacagt
     3841 cggcttgacc ggcgggacgg gaggaggccg agtggaggac gagacgtcac cggagacgac
     3901 gcacgacctg ctgctgtcgt tggccggccg cgtggacgac gacctgctcg cctgggcgcg
     3961 ggagctggcc gccgtgggcg aggacgcccg tgcggtcgag ctcctgaccg cgagcctggt
     4021 ggctgcccgc accgtgctgc ccgatccggt gcgcgccgga ctcgtcgcgg cggcccggtc
     4081 cgcgcgcatc gacctcgatc ccgccgccgc gctgccgccg tcgcgcgcgg agcagggttc
     4141 cgcgcaccgg ttcgacgccg gtccgcccga tgacgtcgtc agcgcggtcc tgctggctgt
     4201 gcccgcccgc caggtacagg gctgccgcat cctggtcgcg cgccgcctga cgcccgccgg
     4261 ctcggcgccc ggaccgctgc cgcacccggt ggtgctcgtc gaggtcctgc ccggtgcccg
     4321 cgccacggac gtgctggcct accagctcgc cgccaccctc gaacgggccg gcgtccccgc
     4381 gtcggtcgag gtcctcacgg cggacgcgcc cgttcccgcc taccacgccg ctgctctccg
     4441 ccgcgcccgc ctgatccggt cggacgacgg cggcgagcgg cacatggccg aaccggaatg
     4501 ggcgccgggc cgcccctctg cggtgttcgg tgccgggccg gcgtcgatgc ctggccaccg
     4561 ccacgtacgc catccgctgg ctcctcccca gcccgacgtc gcgggcctgc tgggcacccc
     4621 ggcccgaccg ggtgacctgc ggggcgaccc gcgcccagca ccggctgccg acgcgctccc
     4681 gcagcggacc ccgggcccga accccgagcc ggtggacccc gaggcgggca ccctgtcgtt
     4741 gccggccgtt ccgaaccgcg gcaccccgct ctcagccgtc cgccccgagg ccagcgaccc
     4801 gccgcccgtg cggcccgacg cggagccgag cgcggcgtcc gtcgacccgc cggagggcca
     4861 cgagcctgac gcacctcccg gcccctcgtc gccgtccggc ccgccgaccg ggcaggaccc
     4921 gcggccgcga ccgggcccga ctgcacgcac cctgcgcccc cctggcaccc cggacgcccg
     4981 gcctcccctg cccagcccga ttccgctggt gcgccgcaac gggcccacgc cgaccgcgca
     5041 cctgttcgaa cccgtcgccg aacagcccgg cgaggccgcg gaacagctgt tcggcgagca
     5101 gatccagcgg ccggccgagg cggtggacaa ccctcggccg ccccggaaca ccgccatgga
     5161 cacgcccctc ccacaccgga tgaacggcct gtcgcgcacg ctgggcgaga cgcctgcctt
     5221 cgactccctg aacgatcccc tcagcgggcc gctgagcgag ccgctgctgg caccgctgct
     5281 cgaccccacg atccgcgagg acgacccgct cggcgtcgat ggaaggatgc ccgccgcgcc
     5341 cgagccggcc cgcgccgagg aggactggtc gtcggaatgg cagagcgggt catgggcgat
     5401 gtccccgtcg gcgctcgacg acgacgagcc ggaggacacg tccgacgcat cccccgagat
     5461 agccgcgcct gacgtcgagg agcgccctga tgcgcggcct gcgcctcggc gcgccgcccg
     5521 gcaccggtat gtcgacgagg agggcgatgc cgacggcgag ggcaggccgc cggagcccga
     5581 ggccgaggcg ccccgagaca ggtctcctga ggcccagccc gctcccgagc ccgagccggt
     5641 ggccgagccg gtagccgagg acgctccggc agccgcgccc gagccggacg gcacgcccga
     5701 tcccgttccc gcgtcggccg acctcgggct gcgcccggag tcgctggcac ggctgagcga
     5761 tgccgaccgc caactgctcg cccggttgca ggccgagctg ctcgatggcc gcaagccacg
     5821 ggtcagccgc cgggccggta tcgcgaacgg gtcgggcacc aacgggtcga gcggcaacgg
     5881 ctcgggcatc aacggggccc cccgggccga cccgcccgac ctcgccggct gacgcctcag
     5941 ccggccgcgc tcctgacctg ctcgacgagc tcctgcagcc gcaggccgcg cgcgacgtcc
     6001 agcgccggcg gcggccctga tccgtcgatt gccgcgacga gctcgtcgag cagcgcggcg
     6061 aagcaggtgc gcgggtcggc gtgacggccg gcgagccggt ggcgcccgtt cggcccgaac
     6121 acggcgaact cgatctccga ggggtcgatg ggcagccgca tcgacaacgt cacggtgctg
     6181 tgtgccccgc cctcgtggac caacccgaac cgccagaggt cgggctggtc atgatgggcc
     6241 cagcccaccg acgtgaccgc tccgacggcg gcgtcgagca ggtcgatcac gtgggggccg
     6301 aggtcgagga gcgccccgtg ctcgatacgc catgccgagg cggagtacgg gcccccgagc
     6361 agcgaccctg acagccagcg agcggaggcg accgtatccg ggccggccgg ggccgagtgc
     6421 agagcggcga gccactcgcg tacggcgggg gcgtgccgca tcgtcagcac catcgagctg
     6481 tggacgcccg cacgctgcac ggcgtccacc accgcgcgcg cgtcctcgac ggaaccggcg
     6541 aggggcttct cgcagatcag gtgccgaccg actgcggcgg cccgcacggc cagctcaccc
     6601 tggacctgcg ggggcacggc gaaggcgacg acgtcgacat cgtcgaggag gtcgtcgagc
     6661 cgggcgtgcg cgcgcgcccc gaactcctcc gcgacctcgt gagcgaactc gggtctgcgg
     6721 gtccatacgg ccgccatcgc ggtgccggga tgcgccgcga gcgcaggccc gtgcaccgtc
     6781 cgggcccagg gcccgccacc caccagaccg acccgcagct gatccaccga acccatcctg
     6841 cacggtcccg ggttagtgtc cggccatgcc ctccgagccc gtcgtcccga gacctgcggc
     6901 gaccgtcatg ctcgtgcgcg acaaccccga tcccccacca ggacgcacgc cgctgcaggt
     6961 cttcctgcaa cggcgggacc ccggcatggc gttcgcgccc cggatgaccg tctttcccgg
     7021 cgggggtgtc gacgcgaccg accacgacgt gctcccgtcc tgggccgggc cagatccgcg
     7081 ctggtggggc gagcggctcg actgcgacgc cacgctcgcc ggcgcgctcg tacaggccgc
     7141 ggtgcgggag acgttcgagg agtgcggagt gctgctggcc gcgccggagc tcccggcccg
     7201 gctggcgggc taccgcgccg acctcgtcgc gcggcggctg acgttcgcgc aggtgctcgc
     7261 cgagaccggc ctcgtcctgc gtgcggacct gctccgcgcg tgggcgcgct ggatcacccc
     7321 gccgacctca ccccggcgct acgacaccgt gttcttcgtc gcgcgcgtcc cggaggggca
     7381 ggaggccgac gcacacacca ccgaggccgt cgaggccacg tggtggcacc ccgccgaggc
     7441 cgtggagcac tggcagcgcg gggagatcca gctgatggca ccgacgttcc gcacgctgca
     7501 ggagatcgcc gagcaccccg acagcggcgc ggtgctggcg gctgccgacg accgcgtcgt
     7561 acggccggtg atcccgcggg tgcgacggga gggcgagcag atcgtggttg tgctgcccga
     7621 cgacccggac ttccacatcg ccgtcgacca cctcggcccg ggagcgagcg catgagccac
     7681 ccgctgtacg ggaagcttcg gcccgtgacc ccgctggcgt cggtgttgct cgcgcagaac
     7741 ccgtccccga tgacgctcga gggcacgaac acctgggtgc tgcgtgcacc gggcgtggag
     7801 gagtgcgtgg tcgtcgaccc cggcgaggcg gacgaggagc acctgggtcg ggtcgccgcg
     7861 cacgggcccg tggcgctcgt gctcctcacc caccgccacc acgaccacgc gggcggcgcg
     7921 cggcgcttcg ccgagctcac cggcgcaccg gtgcgggccc tggatccctc gctggtcctg
     7981 ggctccgagg cactcggcga cggggacgtc gtggccgtcg ccggggtcga ggtgcgggtg
     8041 gtgggcaccc cggggcacac gtcggactcg ctctcgttcc tgctcgacgg ccccgactcg
     8101 gagacggctg tcctcaccgg cgacacgatc ctgggccggg gcaccacggt catcgcccac
     8161 cccgacggcg ccctcaggcc gtatctggac tccctacggc ggctcgccga gctcctcccc
     8221 ggcaccgccg tgctgcccgg gcacgggccc gagctcccgg acgccccggc ggcggcggcc
     8281 gcctacctgg cccaccggga gcagcggctc gagcaggttc gcggggcact cgggcggctc
     8341 ggcccggacg cgacggcgcg gcaggtggtg gagctggtct atgccgacgt ggaccgggcg
     8401 ctctggcccg ctgccgagtg gtcggtgcga gcgcagctgg actacctgcg cgcctcggcc
     8461 tgagacccgc tctaggcggc caccggggcc ggggtcgtcg tcggggcgag ggtgccgcgg
     8521 cgcagccaga gcaccacggc gagcaccacg ccggccgagc cgctcgccag gaaggccgca
     8581 ggcggtccgg ctgcctctac cacgatcccc gccatcgact gcccggcggc gagcccgagc
     8641 gtggccgcgg tgaccaccca gccgaacgcc tccgtcgccg tgcccgcagg tgcggtcgcc
     8701 tccacaccga gggagtgcgc ggtgacctgg ggcgtgatcg tggcgcccgc cagcagcatc
     8761 gcgacgacca gcgcaggcag cgggcccgcc accgccatcg caccgacgca caccgcgaac
     8821 gccagcagca gcaccggcaa ccgcaggtgc aacgggcgcg gccagggccg cagcgagtag
     8881 gcgacgcccg ccagcaccga cgcgaccgac catgccgaca gcagcacccc gccgagggcc
     8941 gccgagcccg cggcggccgt caccgcgggc acgccgacct cgacggtgcc gatgaccagg
     9001 ccgaacccca gcgacgcgag cacgaccgtg cggaggcccg gcacggcgag ggcgccgaac
     9061 acgcctgcgg gtcgggcggg cacggggtgg tgcgtgcgga ccgcgtgggt gacggcgaag
     9121 ccgaccgagc ccgtcacgat cccgacgagc gcgaccgcga tcaggacgat gctcgtctcg
     9181 ag
//
LOCUS       DASRSC010000343         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2437391,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000343 DASRSC010000000
VERSION     DASRSC010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2437391"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VFE39_16735"
     CDS             <1..717
                     /locus_tag="VFE39_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005162959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET6260284.1"
                     /translation="GQRWTSLEELAKAGHRIDVTELEPDLYSLTATPDVGIGQHAKLV
                     RTPAGNLLWDPLGYLDDGAVRRVRELGEVAAIVASHPHMFGVQVEWSRALGGPPVLVA
                     ERDLEWLARPDPVIRPWSGKVELLPGVTLTQPGGHFPGSAVAHWAAGADGTGVLLSGD
                     TIMPNPDRTSVSFMRSYPNRIPLSGAVVERVSQHVEGFEFERLYGNFGAVIDADARAI
                     VRRSADRHIGWVRGDFDHLT"
     gene            767..1807
                     /locus_tag="VFE39_16740"
     CDS             767..1807
                     /locus_tag="VFE39_16740"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET6260285.1"
                     /translation="MTDVTSRTKPLRISFLAFVEHSGVSGAHSDGLREGFRLFERAEA
                     LGYDAGWVRTRHLEPYLSAPLPFLSAVSQRTQRLRLGTSVVPIRYENPVRLAEDAATT
                     DLIAEGRLELGLSSGYAHNEALFGPLYGPIDRDFTDEVDARLRQFLDAVRGGTLAVAD
                     ARTGFAPEGTPLTVQPVSPTLSDRISYGAGRLASAVRTGDLGLGLQLSTLNTEITDLS
                     FEDSQARTITAYRQAHREATGRDGYVSVGRMILPVLREADREAYAWLIERSEQRQRAH
                     GTPEAPPLHFGIAHSGSPESITAALSRDAGLQAADELVVVLPFGHDPAVSHRILETVI
                     GEVTPALGMHAA"
     gene            1972..>2491
                     /locus_tag="VFE39_16745"
     CDS             1972..>2491
                     /locus_tag="VFE39_16745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6260286.1"
                     /translation="MRSRDTRTAYLLLAPSLFGVAAFLVLPILVVVWMALQRWDLIGP
                     STFVGLDNVVAVAQDGRFARSLLVTVVFVAIVIPAQTVLGLVAALLLNRGLRGSAVFR
                     VLYVLPWICAPLVLGVVWRWILAPTDGALNALLGRRIEWLADPALALPSVAAVTVWTQ
                     VGYVALFFVAGLR"
BASE COUNT          327 a          899 c          887 g          378 t
ORIGIN      
        1 gggcagcggt ggacgagcct cgaggagctg gccaaggcgg gccaccggat cgatgtcacc
       61 gagctggagc cggacctgta ctccctcacg gccactcctg acgtcggcat cggtcagcac
      121 gccaagctgg tccgcacgcc ggccggcaac ctgctgtggg acccgctcgg ctacctggac
      181 gacggcgccg tccggcgggt gcgtgagctg ggcgaggtcg ctgcgatcgt ggcgagccat
      241 ccgcacatgt tcggcgtgca ggtggagtgg agccgggcgc tgggcgggcc tccggtgctg
      301 gtcgccgagc gggacctgga gtggctggcc cgccccgatc cggtgatccg cccgtggtcg
      361 ggcaaggtgg agctccttcc cggggtgacg ctgacccagc cgggcgggca tttcccgggc
      421 agcgcggtcg cacactgggc ggccggggcc gacggcacgg gggtgctgct cagcggcgac
      481 acgatcatgc cgaacccgga ccgtacctcc gtcagcttca tgcgcagcta cccgaaccgg
      541 atcccgctgt cgggcgccgt ggtcgaacgg gtcagccagc acgtcgaggg cttcgagttc
      601 gagcggctgt acggcaactt cggggccgtg atcgacgcgg acgcgcgcgc catcgtgcgc
      661 cggtcggccg accgccacat cggctgggtc cggggggact tcgatcatct gacctgagta
      721 ggggttgatc acagggcagc gatcccggtg ctttcgctgt gctgagatga cggacgtgac
      781 gtcccggacg aaacccctgc gcatcagctt tctcgccttc gtcgagcact ccggcgtgag
      841 cggcgcgcat tccgacgggc tgcgggaggg cttccggctg ttcgagcgcg ccgaggcact
      901 ggggtacgac gcgggctggg tccgcacccg gcacctcgag ccctacctgt ccgctccgtt
      961 gccgttcctg tccgcggtgt cgcagcgcac gcagcggctg cgcctgggca catccgtcgt
     1021 gccgatccgc tacgagaacc cggtgcggct cgcggaggac gccgcgacga ccgacctcat
     1081 cgccgagggg cggctcgagc tggggctgag ctccggctat gcgcacaacg aggcgctctt
     1141 cggcccgctg tacggaccga tcgaccgcga cttcaccgac gaggtcgacg cacggctgcg
     1201 gcaattcctc gacgccgtcc gtggcggcac cctggcggtg gccgacgcgc gcacgggctt
     1261 cgcgccggaa ggcactccac tcaccgtcca gccagtgagc cccacgctgt ccgaccgcat
     1321 ctcctacgga gcgggccggc tcgccagcgc cgtgcgcacc ggcgacctcg gtctcggcct
     1381 gcagctctcg acgctcaaca ccgagatcac cgatctgagt ttcgaggact cccaggcccg
     1441 gacgatcacg gcataccggc aggcgcaccg ggaggcaacg ggccgcgacg ggtacgtcag
     1501 cgtcggccgg atgatcctcc ccgttctgcg cgaagcagac cgcgaggcct acgcctggct
     1561 catcgaacgc tcggagcaac ggcaacgggc gcacggcacc cccgaggcgc caccgctgca
     1621 cttcggcatc gcgcactccg gctcaccgga gagcatcacc gcggcgctct cccgcgacgc
     1681 cgggctgcag gcagccgacg agctcgtcgt ggtgctgccc ttcggccacg acccggcggt
     1741 ctcgcaccgg atcctcgaga cggtgatcgg cgaggtgacg cctgcgctcg gcatgcacgc
     1801 agcctgaccc gcggcccgcg gcagtccttg acagcaacgc ggggacgatc ttagctccgg
     1861 tgacgaatcg accgcggtcg cgatcaacct cgggggacgg cagtgacggc aggacacaac
     1921 ggtccggctc cccccaggcg cccaggccgc cggccgcgcg gatccgccct cgtgagatcc
     1981 cgggacacac gcacggccta cctgctgctc gccccgagcc tgttcggcgt ggcagcgttc
     2041 ctggtgctgc ccatcctggt cgtggtgtgg atggccctgc agcgatggga cctcatcggc
     2101 ccaagcacgt tcgtcggcct cgacaacgtc gtcgcggtgg cccaggacgg ccggttcgcc
     2161 cggtcgttgc tggtcaccgt ggtgttcgtg gcgatcgtga tcccggcaca gaccgtgctg
     2221 ggcctcgtcg cggcgctgct gctcaaccgc ggcctgcgcg gctcggcggt cttccgggtg
     2281 ctctacgtgc tgccgtggat ctgcgcgccg ctggtgctcg gggtggtgtg gcgctggatc
     2341 ctcgccccca ccgacggcgc cctgaacgcc ctgctcgggc gccgcatcga gtggctggcc
     2401 gacccggcgc tcgcgctgcc gtccgtcgcg gcggtcaccg tgtggacgca ggtcggctac
     2461 gtcgcgctgt tcttcgtcgc cgggttgcgc g
//
LOCUS       DASRSC010000344         6276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1128037,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000344 DASRSC010000000
VERSION     DASRSC010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1128037"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VFE39_16750"
     CDS             <1..405
                     /locus_tag="VFE39_16750"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HET6260287.1"
                     /translation="DRDDDRLLANVTVFRLDDVFNPTGGEIGYWAHPDARGRGIVVEA
                     VDLVVAHAFSPVRAGGLGRHRLQIGAAWSNAARRQVAERAGFTFTARYRQDGVIGYGE
                     GRTLDDGAGYERLATDGSSRASRTSGVRSRRG"
     gene            complement(386..1558)
                     /locus_tag="VFE39_16755"
     CDS             complement(386..1558)
                     /locus_tag="VFE39_16755"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET6260288.1"
                     /translation="MHRRTAAVRPPSRRLVRSLAVVAALALSVAACTTTGPGTNSSAP
                     ADGPQRVSLQLQWSTQGQFAGYLAAVDQGFYTKRGLDVQIKEGGVDIVPQTVLAQGQA
                     DYAIAWVPKALASREQGAEITDVAQIFQRSGTYQVSFANKGIASAANLAGKRVGNWGF
                     GNEFELFAGMTAAGLTPATDVTLVQQQFDMQALLSGEIDAAQAMSYNEYAQVLEAKNP
                     ATGQLYTPADFSVINWNDTGTAMLQDAIWASTQRLADPAFQDTTTRFIAASIEGWAYC
                     RDNVASCRDIVVKAGSTLGASHQLWQVNEVNKLIWPAPAAGAGAIDPAAWAQTVRVAR
                     TAVNADGQTVLTRDPDPEAYTNTYVERAVGTLKAEGVDVVGSGYTPTTVTLNPGGS"
     gene            complement(1582..2379)
                     /locus_tag="VFE39_16760"
     CDS             complement(1582..2379)
                     /locus_tag="VFE39_16760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6260289.1"
                     /translation="MSAGRSERLAARGGALRVARVWPAVAFGITGLVLWELVVVLGRV
                     PAFLLPPPSAIVAQIGAQAPVIVATTAVTGGNAAVGLVAGFVLGVALALLAARSRLFQ
                     DLATPVVLAASAVPIVALAPVFTTMFGSTTEVARRLVVTVLVIVPVFVSTARGLRQVQ
                     PVHAELMTSLAATPWQHARFVRLPGAVPYIMTGLRLAAPAAVIAAIVAEYFGGLQNGL
                     GSRISSAAANTAYARAWAFVAASIALGLLFHLAGLLAERLVTRGLRT"
     gene            complement(2412..3203)
                     /locus_tag="VFE39_16765"
     CDS             complement(2412..3203)
                     /locus_tag="VFE39_16765"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6260290.1"
                     /translation="MTSGGAVTLAGVEKHFGDVHALDGIDLEVCAGEFVSLIGPSGCG
                     KSTLLRIVADLEHPTGGTALVGGKTPQQARLDQDYGIAFQQAGLLEWRSVTENVELPL
                     HVHGVGKADRRARAAELLELVGLHEFAGSRPSELSGGMQQRVAIARALAPSPRLLLMD
                     EPFGALDEMTRERMQAELLRIIGETGAAVLFVTHSIPEAVVLSDRVVVMSPRPGRITE
                     IVPGRRPGARAATDLVPDDVEDVRESGAFFAAITAVREALRKGHE"
     gene            complement(3200..4069)
                     /locus_tag="VFE39_16770"
     CDS             complement(3200..4069)
                     /locus_tag="VFE39_16770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HET6260291.1"
                     /translation="MGSRSRTVLTTVAAFVLLVTLWELAKLVLPADGVTVGGTRVLPR
                     TDDGSMPHVWTVLGRLAEPEVAGAATTRTVAAAVASGALFTLGLAMGGLVLGGVLGVL
                     LALVMQRFRLVERAALPYVVASQTVPLIAIAPLVAGWGGNIAILGWSWQPWASVMVIS
                     SYLAFFPIAVGMLRGLTSPARADVELFRTLASGWWTTLVRLRLPASVPHVVPAIRLAA
                     AAAVVGAIVAEISTGTRGGIGRLIIEYAQTATGDPSRMYTAILGAAVLGLVAAGAVGL
                     LDMGLRRYRGSVS"
     gene            complement(4198..4935)
                     /locus_tag="VFE39_16775"
     CDS             complement(4198..4935)
                     /locus_tag="VFE39_16775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6260292.1"
                     /translation="MVGTVLVLGGRSEIGVAVARRLAPGATVVLAARRSADLDVEEKA
                     LLDAGAAAVDRIEFDADDIAAHRTVLREVVSRHRRLDVVVVAFGVLGDQTRAEHDPGH
                     AVEVVHTDYVAHVSVLTHLAAILRIQGCGTLVVFSSVAGVRVRRANYVYGSAKAGLDG
                     FTSGLADAMHDSGARVLLVRPGFVIGRMTEGMRPAPFSSTPDQVADATVRALQSGRDE
                     VWVPTVLRPVFAVLRHAPRALWRRLPR"
     gene            5022..5291
                     /locus_tag="VFE39_16780"
     CDS             5022..5291
                     /locus_tag="VFE39_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSMEG_0570 family nitrogen starvation response
                     protein"
                     /protein_id="HET6260293.1"
                     /translation="MIFEVRWPDGSARPYYSPSLVVEEYLTVGESYPVADFVSRSREA
                     LGIASERVREKYGIPCSRAAASLAAIELTAATFADGTVRVERLSR"
     gene            5370..6203
                     /locus_tag="VFE39_16785"
     CDS             5370..6203
                     /locus_tag="VFE39_16785"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET6260294.1"
                     /translation="MRAAVLGFPVAHSLSPVLHTAAYAALGLDDWRYELHECDEAGLA
                     GFVAGLDREWAGLSLTMPLKRVALDVAAEASPLAMATGAANTLVLRDGRRFADNTDVA
                     GIAAALQGAGVGGAGTEAGRAVVLGAGGTAQAALAALHELGISDVDVLVRSVERAGEL
                     RAAADRIGSAPAVSDALADPTRATAAIVGAHVVISTLPSGAADAIAGAGPDTVLLDVV
                     YAPWPTRFAATAAAAGAQVVSGLEMLLHQAVAQVELMTRHTAPVADMRAALDEAVAVH
                     P"
BASE COUNT          921 a         2345 c         2221 g          789 t
ORIGIN      
        1 gaccgcgacg acgaccgcct gctcgccaac gtgaccgtgt tccggctcga cgacgtcttc
       61 aaccccaccg gaggcgagat cgggtattgg gcgcacccgg acgcgcgagg ccgcgggatc
      121 gtcgtcgagg ccgtcgacct cgtggtggcg cacgcgttca gcccggtgcg ggccggcggg
      181 ctcggccggc accggctgca gatcggcgcc gcgtggtcga acgccgcccg ccgccaggtg
      241 gccgagcgcg ccggcttcac cttcactgcc cggtaccggc aggacggagt gatcggctac
      301 ggcgagggcc ggacgctcga cgacggcgcc gggtacgagc ggctcgccac cgacggctca
      361 tcgcgggcga gccgcacgag cggggtcagg agccgccggg gttgagcgtg accgtggtgg
      421 gtgtgtaacc ggaacccacc acgtcgacgc cctccgcctt cagcgtcccg accgcccgct
      481 cgacgtaggt gttggtgtac gcctccgggt cggggtcgcg cgtgagcacc gtctgcccgt
      541 cggcgttgac ggccgtccga gccacccgca ccgtctgcgc ccacgccgcc gggtcgatcg
      601 cgcctgcccc cgcggccggt gcaggccaga tcagcttgtt gacctcgttg acctgccaca
      661 gctggtggct ggcgccgagc gtcgatccgg ccttgacgac gatgtcccgg cacgacgcga
      721 cattgtcccg gcagtaggcc cagccctcga tcgaggcggc gatgaaacgc gtggtggtgt
      781 cctggaaggc ggggtcggcg agccgctgcg tgctggccca gatcgcatcc tgcagcatcg
      841 cggtgccggt gtcgttccag ttgatcaccg agaagtcggc gggcgtgtag agctgccctg
      901 tcgcggggtt cttcgcctcg agcacctgcg cgtactcgtt gtaggacatg gcctgcgcgg
      961 cgtcgatctc gccggacagc agcgcctgca tgtcgaactg ctgctgcacc aacgtgacgt
     1021 cggtggccgg ggtgagcccg gccgccgtca tgcccgcgaa gagctcgaac tcgttgccga
     1081 agccccagtt gcccacccgt ttgccggcca ggttcgccgc ggacgcgatc cccttgttcg
     1141 cgaacgacac ctggtaggtg cccgagcgct ggaagatctg ggcgacatcg gtgatctcag
     1201 cgccctgctc ccgcgacgcc agcgccttgg gcacccaggc gatcgcgtag tcggcctggc
     1261 cctgcgccag caccgtctgc gggacgatgt cgaccccgcc ctccttgatc tgcacgtcga
     1321 gcccgcgctt cgtgtagaag ccctggtcga cggccgcgag gtagccggcg aactgcccct
     1381 gggtggacca ctgcagctgc aatgacaccc gctgcggccc gtcggccgga gccgagctgt
     1441 tcgtgcccgg ccccgtcgtc gtgcacgcgg cgacggagag agccagcgcg gcgaccaccg
     1501 cgagcgagcg cacgagtcgt cgggacggcg gccggacggc cgctgttcgt cggtgcatga
     1561 ggacctccgg ggatggttcg atcaggtgcg aagaccgcgg gtcaccagcc gctcggcgag
     1621 cagcccggcg agatggaaca gcagccccag tgcgatggag gcggcgacga aggcccaggc
     1681 gcgtgcgtaa gcggtgttgg ctgctgccga ggagatcctg ctgcccagac cgttctgcag
     1741 gccgccgaag tactccgcga cgatcgcggc gatcaccgcg gccggtgcgg cgagccgcag
     1801 gccggtcatg atgtacggca ccgctcccgg cagccggacg aaccgcgcat gctgccacgg
     1861 cgtggcggcg agcgacgtca tcagctcggc gtgcaccggc tgcacctggc gcagcccgcg
     1921 cgcggttgag acgaacacgg gaacgatcac caacaccgtg acgacgagcc ggcgcgcgac
     1981 ctccgtggtg gagccgaaca tcgtggtgaa cacgggggcg agcgccacga tcggcacggc
     2041 gctcgcggcc agcaccaccg gcgtcgcgag atcctggaag agccgggacc gcgctgctag
     2101 cagcgccagc gccacgccga ggacgaaccc ggccaccagc ccgaccgccg cgttgccccc
     2161 ggtgacggcg gtggtggcga cgatgaccgg ggcctgggca ccgatctgcg cgacgatcgc
     2221 gctcggcggg ggcagcagga aggcgggcac ccggcccagc accaccacca gctcccacag
     2281 caccagccct gtgattccga acgccacggc cggccagacc cgggccaccc gcagcgcacc
     2341 cccccgcgct gcgagccgct cgctccggcc tgcgctcacg cccgccgcac cgggtaccgc
     2401 gcccgcccgt gtcactcgtg ccccttgcgc agcgcctcgc gcacggccgt gatcgcggcg
     2461 aagaaggcgc cggactcacg aacgtcctcc acgtcgtcgg gcacgagatc tgtggccgcc
     2521 cgggcgccgg gcctgcggcc cggcacgatc tcggtgatcc ggcccggccg cggcgacatc
     2581 accaccaccc gatccgacag caccacggcc tcgggaatcg agtgcgtcac gaacagcacg
     2641 gccgcaccgg tctccccgat gatccgcagc agctcggcct gcatccgctc gcgggtcatc
     2701 tcgtccagcg ccccgaacgg ctcgtccatg agcagcagcc gcgggctggg ggcgagcgcg
     2761 cgggcgattg cgacgcgctg ctgcatcccg cccgacagct cgctcgggcg ggacccggcg
     2821 aactcgtgca agccgacgag ctcgagcagc tccgctgccc gcgctcggcg gtccgccttc
     2881 ccgacgccgt ggacgtgcag cggcagctcg acgttctcgg tgaccgaccg ccactccagc
     2941 aggccggcct gctggaaggc gatgccgtag tcctggtcga gccgtgcctg ctgcggcgtc
     3001 ttgccgccga ccagcgcggt gccaccggtc gggtgttcca ggtcggcgac gatgcgcagc
     3061 agcgtcgact tgccgcagcc agacggcccg atcagcgata cgaactcgcc cgcgcacacc
     3121 tccaggtcga tgccgtccag cgcgtggacg tcaccgaagt gcttctccac cccggccaac
     3181 gtgacggccc cacccgatgt cacgagacgc tcccccggta gcggcgcagc cccatgtcca
     3241 gcaggccgac cgcacctgcg gcgacgagcc cgagcacggc ggcgccgagg atggcggtgt
     3301 acatccgcga cgggtcgccc gtggcggtct gggcgtactc gatgatcagg cgtccgatgc
     3361 cgccgcgggt gccggtggag atctccgcga cgatcgctcc cacgacggcg gccgcggcgg
     3421 cgagccggat cgcgggcacc acgtgcggga ccgacgcggg cagccgcagg cgcaccagcg
     3481 tggtccacca gcccgacgcc agggtgcgga acagctcgac gtcggcgcgg gcaggcgagg
     3541 tgagcccgcg caacatcccc acggcgatgg ggaagaacgc caggtaggag gagatgacca
     3601 tgacactggc ccacggctgc cacgaccagc cgaggatcgc gatgttgccg ccccagccgg
     3661 cgacgagtgg ggcgatcgcg atgagcggca ccgtctgcga cgcgacgacg tagggcaggg
     3721 ccgcccgctc cacgagccgg aagcgctgca tgaccagggc cagcagcaca ccgagcaccc
     3781 cgccgagcac cagcccgccc atcgcgaggc cgagggtgaa cagcgcgccg gacgccacgg
     3841 cagcggcgac cgtgcgcgtg gtcgcggcac cggcgacctc gggctcagcc agccgcccga
     3901 gcaccgtcca cacgtgcggc atcgacccgt cgtcggtgcg aggcagcacc ctggtgccgc
     3961 cgaccgtgac gccgtcggcg ggcagaacga gcttcgccag ctcccacagg gtgacaagca
     4021 ggacgaatgc cgcgacggtg gtcagaaccg tgcgcgaccg acttcccatc gggaggctcc
     4081 tgacgctcgg ccgaacccgg agcgctcccc gggtggtgga aggcgatact gcgagccgcg
     4141 gatgaccgta gcgttaccgg cgcgttccca gtagtgcctg ctgctgcgca tgcacgttca
     4201 ccggggcagg cgtcgccaca gcgccctcgg cgcgtgccgg agcaccgcga acaccgggcg
     4261 cagcaccgtc ggcacccaga cctcgtcccg cccgctctgc agcgcgcgca ccgtcgcgtc
     4321 ggccacctga tccggagtgg acgagaacgg agccggcctc atcccctcgg tcatccgccc
     4381 gatcacgaag ccgggacgca ccagcagcac ccgggcgccg ctgtcgtgca tggcgtcggc
     4441 gaggccgctg gtgaaaccgt cgaggcccgc cttcgccgac ccgtaaacgt agttggcgcg
     4501 gcggacccgc acaccggcga ccgaggagaa caccacgagc gtgccgcacc cctggatgcg
     4561 caggatcgcg gcgaggtggg tgagcaccga gacgtgcgcg acgtagtcgg tgtgcacgac
     4621 ctccacggca tgtcccgggt cgtgctcggc cctggtctgg tcgccgagca cgccgaaggc
     4681 gacgaccacg acgtcgagcc tgcggtgcct gctcaccacc tcgcgcagaa cggtgcgatg
     4741 ggcggcgatg tcgtcggcgt cgaactcgat gcggtcgacc gcggcggccc cggcatcgag
     4801 aagcgccttc tcctcgacgt cgaggtccgc gctccgccgc gctgcgagca cgaccgtggc
     4861 ccctggcgcg agcctgcgcg ccaccgccac tccgatttcg ctgcgtcccc cgagcaccag
     4921 cactgtcccc accaccgcgg cagcctggca taccgcgccg acagagctgc ggcgacacat
     4981 cagggcaaca cgacgaacct agcctggcgg gcgtgccgga gatgattttc gaggtccgct
     5041 ggcccgacgg gtcggcgagg ccctactact ccccatccct cgtggtagag gagtacctga
     5101 ccgtcggcga gtcgtatccc gtcgccgatt tcgtgtcgcg cagccgggag gcgctcggga
     5161 tcgccagcga gcgggtgcgg gagaagtacg ggataccctg ttcacgcgct gcggcgtcgc
     5221 tggccgcgat cgagctgaca gcagcaacct tcgccgacgg cacggtgcgc gtcgagcggc
     5281 tgagccgctg acgcccgacc ccgccggatc cggactccct cgtgaccccg ccgatactcg
     5341 gggctgcacg tcgagcacat acttgcccga tgcgcgccgc cgtcctcggc ttcccggtcg
     5401 cccactcgct ctcgccggtc ctgcacacgg ccgcgtacgc cgcgctcggc ctggacgact
     5461 ggcggtacga gctccacgag tgcgacgagg ccggcctggc gggcttcgtc gcggggctcg
     5521 acagggaatg ggcggggctg tccctgacga tgcccctcaa gcgggtggcg ctcgacgtcg
     5581 ccgcggaggc ctcgccgctg gcgatggcca ccggcgccgc aaacacgctc gtcctgcgtg
     5641 acgggcgccg gttcgccgac aacaccgacg tcgccgggat cgccgccgcg ctccagggcg
     5701 ccggggtggg cggggccggc accgaggcgg ggcgcgccgt ggtgctcggc gcaggaggca
     5761 cggcgcaggc tgccctcgcc gcgctgcacg agctggggat ctccgacgtg gacgtgctgg
     5821 tccgctccgt cgaacgggca ggcgagctgc gcgccgcggc cgaccggatc gggtcggcgc
     5881 ctgccgtcag cgacgcgctg gccgacccga cccgcgcgac ggctgcgatc gtcggagccc
     5941 acgtcgtgat ctccacgctg ccctccggag ccgccgacgc catcgccggt gcaggcccgg
     6001 acaccgtcct gctcgacgtg gtctacgcgc cgtggcccac acgattcgcc gccacggcgg
     6061 ccgcggccgg agcgcaggtg gtgagcgggc tggagatgct gctgcaccag gccgtcgcac
     6121 aggtggagct gatgacgcgc cacaccgcac cggtggcgga catgcgtgcc gctctcgacg
     6181 aagcggtcgc ggtacacccc tgacccaccc gcccgcgccc cgcgagtcgc cccaaaacag
     6241 cccgcgagtc gcccgaaatc cgcccgcgag tcgccc
//
LOCUS       DASRSC010000345         4191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2602833,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000345 DASRSC010000000
VERSION     DASRSC010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2602833"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1446
                     /locus_tag="VFE39_16790"
     CDS             <1..1446
                     /locus_tag="VFE39_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HET6260295.1"
                     /translation="GLGVRELRRAAKEMDADETVAALLVELAVAADLVGESDGVAPEW
                     VPTTNADVWAAGGPELRWSLIARTWLDLPRLPGLVGRKDDAGRAISALSEAVRRPLAP
                     RDRRRVLAGLAELAPGVAPGSPAALRDVLAWRAPRRGGRLRDEVVGWALAEGTVLGLV
                     ALDALSTPGRVLLTDSDGLVAALRATLPEPVNHVLLQADLTAVAPGPLEPELARELDL
                     VAEVESAGGATVYRFTDATIRRALDAGRTASELHELLAKRSATPVPQGLTYLVDDVAR
                     RHGRLRGGVAASFLRSDDAVLIAEVLAHSETAALELRRIAPTVVVSPLPLVELLDGLR
                     AAGFSPAAEDTGGAVLDLSDRGRRTAPRRRLPGRSAAPPEPGTEQLAALVSRMRAGDA
                     LAGVRRDAGAPTPANGTIPDLLRSAAAARRSVWIGFVDGHGVAGERVLEPTSVGGGVV
                     EGRDGVDGALHRVPLHRITSIAVVESSPSDS"
     gene            complement(1507..2109)
                     /locus_tag="VFE39_16795"
     CDS             complement(1507..2109)
                     /locus_tag="VFE39_16795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HET6260296.1"
                     /translation="MTRQSGFGQRAVATAATISAKIVVAGGFGVGKTTFVGSVSEIVP
                     LTTEAVMTEASAGVDDLAATPNKVTTTVAMDFGRVSLDSELILYLFGTPGQHRFWFMW
                     DDLVRGAIGAVVLVDTRRLADSFAAIDFFEDRGLPYVVGLNCFDGLLQHRIEDVREAM
                     SIDQSIPIVSCDARIRQSTKQTLIALVEHAMQHRMTAGAR"
     gene            complement(2150..2761)
                     /locus_tag="VFE39_16800"
     CDS             complement(2150..2761)
                     /locus_tag="VFE39_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010305141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF742 domain-containing protein"
                     /protein_id="HET6260297.1"
                     /translation="MNSGPDDERRQSSEPTFADVMNGFSFDAGRSPRRRRWGRRRGAD
                     VELNDDLAADTDDAARPTPPPAQIPPGPPTGPIDIRSIQAEEVEQEVGASAVRPYAWT
                     RGRTKASLELAIETLVSTSQRGRDQIGLLQVEHRAVAELCEQTRSVAEVAALLSLPLG
                     VARVLLGDMAGLGVVTVHQTASSAGNVPDLALMERVLSGLRRL"
     gene            complement(2826..3251)
                     /locus_tag="VFE39_16805"
     CDS             complement(2826..3251)
                     /locus_tag="VFE39_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HET6260298.1"
                     /translation="MTAPQTQPSRFGWLVTNFAERVPGVAHAIVVSADGLLLTASDRL
                     PRDRADQLAAVSSGLVSLTQGAARCFDAGGVVQTVVEMDRGIVLLMSISDGSCLAVLA
                     SPSCDIGLIGYEMTLLVDRVGQLLTPELRAELQGSFGGI"
     gene            3915..4070
                     /locus_tag="VFE39_16810"
     CDS             3915..4070
                     /locus_tag="VFE39_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260299.1"
                     /translation="MGRFERNHAEAISSKIGVVSPMSNSSDFRAEDAEGAGSAMIGML
                     GANNPSG"
BASE COUNT          556 a         1462 c         1570 g          603 t
ORIGIN      
        1 gggctgggtg tgcgggagct gcgcagggcg gccaaggaga tggacgccga cgagacggtg
       61 gcggcgctgc tcgtggagct ggcggtggcg gccgacctgg tgggggagag cgacggcgtg
      121 gcaccggaat gggtgcccac caccaacgcc gacgtgtggg cggccggcgg ccccgagctg
      181 cgctggtcgc tgatcgcgcg cacgtggctg gatctgccgc ggctgcccgg cctcgtcggc
      241 cgcaaggacg acgccgggcg ggcgatctcc gccttgtccg aggctgtccg gcgcccgctc
      301 gccccgcgcg accggcgccg ggtgcttgcc gggctggccg agctcgctcc cggcgtcgcg
      361 cccggctcgc ctgccgcgct tcgagacgtc cttgcctggc gcgcgccacg ccgtggcggg
      421 cgactgcgcg acgaggtggt cggctgggcc ctggcggagg gcaccgttct cggtctcgtc
      481 gccctggacg cgctgtccac cccgggacgg gtgctcctca ccgactccga cggcctggtc
      541 gctgccctcc gcgcgaccct gcccgagccg gtcaaccacg tgctcctgca ggccgacctc
      601 accgccgtgg ccccggggcc gctggagccg gagctcgcca gggagctcga tctggtcgcg
      661 gaggtcgagt cggcgggtgg agccaccgtc taccgcttca ccgacgccac gatccgccgt
      721 gcactcgacg ccgggcgcac ggcctcggag ctgcacgagc tgctcgcgaa gcgctcggcc
      781 acccccgtgc cacagggcct gacgtacctc gtcgacgacg tggcccgccg ccacggccgg
      841 ttgcgcgggg gcgtggcagc ctcgttcctc cgctccgacg acgcggtgct catcgccgag
      901 gtcctcgccc actccgagac cgcggctctg gagctgcgcc gcatcgcgcc gacggtggtg
      961 gtctccccgc tgccgctcgt cgagctgctg gacgggctgc gcgccgcagg gttcagccct
     1021 gccgcggagg acaccggtgg cgccgtgctc gacctctccg accggggcag gcggaccgcg
     1081 ccgcgacggc gtctcccggg tcgctccgct gcgcccccgg agccaggcac cgagcagctc
     1141 gcagcactcg tgtcccggat gcgcgccggg gacgcgcttg ccggcgtgcg ccgggacgcc
     1201 ggagcgccga ccccggccaa cggcacgatc cccgacctgt tgcgttcggc cgccgctgcc
     1261 cgccgcagcg tctggatcgg ctttgtggac gggcacggtg tggccgggga gcgcgtactg
     1321 gagcccacga gcgtgggcgg cggggtggtg gagggtcggg acggcgttga cggcgcccta
     1381 caccgcgtgc cgctgcaccg gatcaccagc atcgcggtgg tcgagagctc gccgagcgat
     1441 tcctgagctc gggcgtccgt cgggagaatc gtccgaacgc ggggctctag accgcctgcc
     1501 gtgcgatcag cgggctcctg cggtcatcct gtgctgcatg gcgtgctcga ccagcgcgat
     1561 gagggtctgc ttcgtggact gccggatgcg tgcgtcgcag ctgacgatcg ggatcgactg
     1621 gtcgatcgac atggcctccc ggacgtcctc gatgcggtgc tggagcagcc cgtcgaagca
     1681 gttgagaccc acgacgtagg gcagcccgcg gtcctcgaag aagtcgatcg ccgcgaacga
     1741 gtcggccagc ctgcgggtgt cgaccagcac cacggcgccg atcgcgcccc gcacgaggtc
     1801 atcccacatg aaccagaacc ggtgctgccc cggtgttccg aacaggtaga ggatcagctc
     1861 cgagtcgagc gacacgcggc cgaagtccat ggccacggtg gtcgtgacct tgttcggggt
     1921 ggccgcgaga tcgtcgacgc ccgcgctggc ctcggtcatg acggcttccg tggtcagcgg
     1981 gacgatctcg gagacggacc cgacgaacgt tgtcttgccg acaccgaagc cgccggccac
     2041 cacgatcttg gccgagattg tcgccgcggt ggcgaccgcc cgttgcccga agccggactg
     2101 acgggtcaac gccgacgcgg agcgaggggg agacccgtca gtggggtaac tagagccgac
     2161 ggagtccact caacaccctt tccatcagcg cgaggtccgg cacgttgccg gcgctgctcg
     2221 cggtctggtg cacggtcacc acgccgagcc ctgccatgtc gcccaagagc actctggcga
     2281 ccccgagcgg cagcgacaga agtgcggcga cctcggcgac agagcgcgtc tgctcgcaca
     2341 gttcggcgac ggcacggtgc tcgacctgca acaggccgat ctggtcccgg ccacgttggc
     2401 tcgtggacac cagtgtctcg atggccagct cgaggctggc cttggtacga ccacgggtcc
     2461 aggcgtacgg ccggaccgcg gaggcgccca cctcctgctc gacctcctcc gcctggatgc
     2521 tgcgaatgtc gatcggcccc gtcggcgggc cgggcgggat ctgcgcgggg ggcggggtag
     2581 gccgcgcggc atcatcggta tcggcggcga ggtcgtcgtt gagctcgacg tctgcgcccc
     2641 gcctgcgtcc ccatctccgc cgtcgcggcg accgaccggc gtcaaagctg aagccgttca
     2701 tgacgtcggc gaaagtcggc tccgaggatt gccggcgctc gtcgtcggga ccggagttca
     2761 tcggacatca cctgccttct accgatcggt gccgtaccgg gggtcgtgat ggggtggtgg
     2821 tctggtcaga tcccgccgaa ggagccctgc agctcggcgc gcagctccgg cgtgagcagc
     2881 tggccgacgc ggtcgacgag caatgtcatc tcgtaaccga tcaggccgat gtcgcagctc
     2941 ggcgacgcca gcactgcgag gcaggagccg tcgctgatgg acatgagcag gacgatcccg
     3001 cggtccatct ccaccaccgt ctgcacgaca ccgccggcgt cgaagcagcg tgcggcgccc
     3061 tgtgtgaggc tgaccagccc ggatgacacg gcggcgagct ggtcggcgcg gtcgcggggc
     3121 aggcggtcgg acgccgtgag caacaacccg tcggccgaca ccacgatggc gtgggcgaca
     3181 cccggcacac gctcagcgaa gttggtgacc agccatccga agcggctcgg ctgcgtctgt
     3241 ggcgccgtca tgaagactcc tcgtcgtgat tcacaccagc gttggcaggt ttagtaccac
     3301 cgttggtccc cgtgccggat tctgctgcct cgccgcgcaa ccggctctcg cgaccttgac
     3361 gaacgccgtg ctggtagctg gcgagccggc cgcggacggt ctcagcgctg cgcgatgagg
     3421 agaccggcgg ggccattacg gcagatcctg cactgcccgg cacgagccgt gctcgtgggc
     3481 gtcgtttcgg cagtccggcg ggcgtcagct cgtcggactg gtcgaccact ccgctcgctg
     3541 cacgccaacc ctcgtccgcc aggctcgcga actcctggtc ccggggcgac accggagcag
     3601 cagcctgcgg tacgaccgca ttctccgtag tcggtggggc aggggtcgga gcctgcgcta
     3661 ccggagccgg agccggagcc ggagccggag ccggagcggg agcgggagcg cgagccggag
     3721 cgggtgcccg tgtgggcgcc ggagccggag ccggagccgg cgccggggtc ggtgccggtg
     3781 cgggagtcgg tgcggggcga gccaccggag ccggcaccgg cgcaggcgac cgggtggggc
     3841 tcggggtcgg gcgggccgcg cgctgtaccg ccggccgctg ttcgccgccc tgctcggtct
     3901 gccagttaac gggtatgggg cggttcgagc ggaaccacgc cgaggcgatc tcctcgaaga
     3961 tcggggtggt ctcgcccatg tcgaactcgt cggacttccg ggcggaggac gccgagggcg
     4021 ccggctccgc gatgatcggc atgctcggag cgaacaaccc gtccggctga acagccgggg
     4081 cgagctgggc ctgctgagca ggctgagcct gctcggcgga ctgagcggac tgagcggact
     4141 gagcgggctg ggccggctgt gcggattgag cgggctgggc cgactgtgcg g
//
LOCUS       DASRSC010000346         6969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4240451,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000346 DASRSC010000000
VERSION     DASRSC010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4240451"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..953
                     /locus_tag="VFE39_16815"
     CDS             204..953
                     /locus_tag="VFE39_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005453051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET6260300.1"
                     /translation="MTKPPHAGSADVAAARLRSIPEASVARLAVYLRMLGELAEQGAE
                     TVSSDELAAATGVNPAKLRKDLSYIGSYGIRGVGYEIAALVRQLERTLGLDHLRAVAL
                     VGVGNLGHALAGYSGFGGRGFPVTALFDVDPDLVGIHINGIVVEHVRAIPQVCAEREV
                     TIGMIATPGPAAQGVCDLLVSSGVRCILNFAPVVLQVPHEVEVRKVDLAVELQILAFH
                     VARKHLAGQDPATVNGHVIEHASNGSGGMLP"
     gene            950..2320
                     /locus_tag="VFE39_16820"
     CDS             950..2320
                     /locus_tag="VFE39_16820"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HET6260301.1"
                     /translation="MSVLVVGLSHRSAPVEVLERAAVMAGEVPKLLDEMLRGTHVSEA
                     VLLSTCNRIEVYAVVDAFHGGLADISAVLSRHAGMPLAELTEHVYVHYAGSAVQHLFA
                     VSAGLDSMVVGEAQILGQLRSAYAAADEAGTVGRVLHDLAQNALRIGKRVHASTGIDA
                     AGASVVSEALADAAAALGGSLEGRRAVVVGAGAMGALAAAHLRRAGAAEIVVLNRSPE
                     RAVRLAENTEAQGTPAHAGGLDALAGELATADLLVACTGAVGTVVDRVTVAAAVADRP
                     ARPLAVCDLGLPRDVEAGVAALPGVTLIDLVALQRRLESDQRGAAVAAAQELVAEEAQ
                     AYLAAQRSAEVTPTVTALRRRASDVIDAELLRLDSRLPDLDLRVREEFTRSVRRVVDK
                     LLHTPTVQVKRLAEGPDGDSYAAALRALFELDPQTPAAVAIQRSGDVIAALEARADIP
                     AQEESR"
     gene            2317..3285
                     /gene="hemC"
                     /locus_tag="VFE39_16825"
     CDS             2317..3285
                     /gene="hemC"
                     /locus_tag="VFE39_16825"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009071975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HET6260302.1"
                     /translation="MTLRIGTRPSALAVAQAGTIADRLSSAGHPAELVTVTTSGDRSG
                     APVAELGVGVFVSALREALVRGEVDVAVHSYKDLPTAPDPRLHMAAVPQREDPRDALV
                     ARDGMVLGELPPGATVGTGSPRRRAQLEALGLGLDVVPIRGNVDTRIGKVRSGELDAV
                     VVAAAGLRRLGRIDEATELLDPLQMLPAPAQGALAIECRVDDLELGRALSLALDDEHT
                     RAAVIAERAVLATLEAGCHAPVGALADVVSDWEPISGDAGGDDNGRVVDRVSLRAVVG
                     TTDGALLRASVTGNLDDAEKLGSALAAELLDMGGLAALGEPLDREI"
     gene            3285..4826
                     /locus_tag="VFE39_16830"
     CDS             3285..4826
                     /locus_tag="VFE39_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional uroporphyrinogen-III
                     C-methyltransferase/uroporphyrinogen-III synthase"
                     /protein_id="HET6260303.1"
                     /translation="MNRAPSTPGRVAFVGSGPGDPGLLTVRAREAIVNAPLVVTDPDV
                     PEVILALVADGAEVRPAVGQPADVARDLVAEARSGRTVTRLVSGDPLTVDSVVQEAIA
                     VAAADVAFDVVPGVPAGTAVPAYAGVPLGSAHIEADVRAGVDWERLAAAPGTLVLHAS
                     PSHLSEVVSALTEHGLAAQTPVAVTASGTATTQKTVQATLATMASEAAELEGPLVITV
                     GAEVGLRAELSWWESRALYGWRVLVPRTKDQAGAMSDRLAVHGATAEEVPTIAVEPPR
                     SPTQMERAVKGLVDGRYQWVVFTSTNAVRAVWEKFAEFGLDARAFSGVKIACVGTATA
                     EKVRSFGIVPELVPSAEESQESSSEGLLSIFPPYDDVLDPVDRVLLPRADIATETLAA
                     GLRDRGWEIDDVTAYRTVRAAPPPAPIREAIKTGGFDAVCFTSSSTVRNLVGIAGKPH
                     ARTIVACIGPQTAETAREFGLRVDVQPDEARVPALVDALAAHAARLRAEGALPPPKKA
                     KARRR"
     gene            4998..5978
                     /gene="hemB"
                     /locus_tag="VFE39_16835"
     CDS             4998..5978
                     /gene="hemB"
                     /locus_tag="VFE39_16835"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HET6260304.1"
                     /translation="MAFPLHRPRRLRSTPALRRLVAETDLRPRHLVLPMFVKEGAGEP
                     VPIASMPGVVQHTRDSLRKAATEAVAAGVGGLMLFGIPERRDAAGSAGVDPDGVLNVA
                     LRDVSAEVGDATVLMADTCLDEFTDHGHCGVLDADGRVDNDATLAVYAEMAVAQAEAG
                     AHLVGPSGMMDGQVGVIREALDAAGHLDTGILAYTAKYSSAFYGPFREAVESQLQGDR
                     KTYQQDGANVREALRELALDLEEGADLVMVKPALAYLDVLRVVAEAADVPVAAYQVSG
                     EYAMVEAAAANGWLDRDRTVLETLTASRRAGADIVLTYWATEVAGRLTIE"
     gene            6088..6558
                     /locus_tag="VFE39_16840"
     CDS             6088..6558
                     /locus_tag="VFE39_16840"
                     /inference="COORDINATES: protein motif:HMM:NF025475.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4282 domain-containing protein"
                     /protein_id="HET6260305.1"
                     /translation="MTYDPGNPGEQPQPPVGQGWSGGQQPPFPGGYGQQPPPGWGPPP
                     GQPGYGGGPTGLGPTGSPQMQAKGFFTALFDFGFNSFATPSVVKVIYVIGMVILGLAY
                     VGFVIAGFTQGAGQGLLVLILGGVVALFYLTLFRITLEFYYAVVRMSEDIHHRR"
     gene            6602..>6969
                     /locus_tag="VFE39_16845"
     CDS             6602..>6969
                     /locus_tag="VFE39_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260306.1"
                     /translation="MSTPSDPQRGDQQPDDGPRVGDGSDGTQEPPSGQQPGEAGQPGE
                     AGQPGQYGQPGPYGQPGQYGQPGQYGQPGPYGQPGQYGQPGPYGQPGQYGQPGPYGQP
                     GQYGQPGQYGSAPYGQPGPYG"
BASE COUNT          955 a         2488 c         2519 g         1007 t
ORIGIN      
        1 gagcacgact gcatccaaga tcgcaacttt gtgcgtgcct tcacaagcga ctaccgtgta
       61 atccagtcga cgtcaacgcc caatcctggg gtactcggcc gggtgtatac cggttggtgg
      121 ggtctgtggt gcggtcgcgc gcatatcagc atcaccggaa aaatcagcac attgagtaca
      181 gcaccatcga ggagacgttg agcgtgacga agccgccgca tgccggctcc gcggatgtcg
      241 ccgccgcgag gctgcggtcc attcccgagg ccagtgtcgc tcggctcgcc gtctacctcc
      301 ggatgctcgg cgaactggcc gagcaaggcg ccgagacggt ctccagcgac gagctcgccg
      361 ccgccaccgg ggtcaacccg gccaagctgc gcaaggacct ttcctacatc ggctcgtacg
      421 gcatccgcgg ggtcggctac gagatcgcgg cgctggttcg ccagctcgag cgcacgctcg
      481 gcctggatca cctgcgggcg gtggcgcttg tcggagtggg caacctgggc cacgcgctgg
      541 cggggtacag cggcttcggt gggcgcggct tcccggtcac ggcactgttc gatgtcgacc
      601 ccgacctcgt cggcatccac atcaacggca tcgtcgtcga gcacgtccgg gcgatcccgc
      661 aggtctgcgc ggagcgggag gtcacgatcg gcatgatcgc cacgcccggt cccgccgccc
      721 agggcgtgtg cgacctgctc gtgagcagcg gcgtgcgctg catcctcaac ttcgcccctg
      781 tcgttctgca ggtgccccac gaggtggagg tccgcaaggt cgacctggcc gtggagctgc
      841 agatcctggc gttccatgtg gcacgcaagc accttgccgg gcaggacccg gccacggtca
      901 acggccacgt catcgaacac gcgtcgaacg gttccggagg gatgcttccg tgagcgtctt
      961 ggtagttggt ctgtcgcacc gcagcgcacc ggtggaggtg ctggaacgcg ccgccgtcat
     1021 ggcgggcgag gtgccgaagc tgctcgacga gatgctgcgt ggcacgcatg tctccgaggc
     1081 cgtgctgctg tcgacgtgca accgcatcga ggtctacgcc gtcgtcgatg cgttccacgg
     1141 cggcctcgcc gacatctccg cggtgctgag ccggcacgcc gggatgccgc tggccgagct
     1201 caccgaacac gtgtacgtgc actacgccgg gtcggccgtg cagcacctgt tcgccgtgtc
     1261 cgccggcctc gactcgatgg tcgtcgggga ggcccagatc ctcggccagc tccgtagcgc
     1321 gtacgccgcc gccgacgagg ccggcaccgt cgggcgcgtg ctgcacgacc tcgcccagaa
     1381 cgcgttacgc atcgggaagc gggtgcacgc gagcaccggc atcgacgccg caggcgcgtc
     1441 cgtcgtctcc gaggcgctcg ccgacgccgc cgccgcgctc ggcgggagcc tggagggccg
     1501 tcgggcggtc gtcgtcggcg ccggggcgat gggagcactt gcggcagcgc acctgcggcg
     1561 cgcgggcgcc gccgagatcg tcgtgctgaa ccgctcaccg gaacgggccg tgcgccttgc
     1621 cgagaacacc gaggcgcaag gcacaccggc ccacgccggg gggctggatg cgctggcagg
     1681 ggagctggca accgccgacc tcctcgtcgc ctgcaccggg gccgtcggca ccgttgtcga
     1741 ccgcgtcacg gtggcggctg ccgtggccga ccgccccgcg cgcccgctcg cggtctgcga
     1801 tctcggcctg ccccgcgacg tcgaggcggg ggtcgcggca ctgccgggcg tcacgctgat
     1861 cgacttggtc gccctgcagc gcaggctcga gtccgaccag cggggcgctg ctgtcgcagc
     1921 cgcccaggag ctcgtggccg aggaggcgca ggcctacctc gccgcccagc gctccgccga
     1981 ggtcaccccc accgtcacgg cactgcggcg gcgcgcgtcc gacgtgatcg acgccgagtt
     2041 gttgcggctc gactcccgcc tccccgacct ggacctgcgg gtgcgcgagg agttcaccag
     2101 gagcgtccgc cgtgtcgtcg acaagctcct gcacaccccc accgtgcagg tgaagcgact
     2161 cgctgagggc ccggacggcg acagctacgc agcagcgctg cgggccctgt tcgagctcga
     2221 cccgcagacc ccggccgcag tggccatcca gcgcagtggc gacgtgatcg cagcgctgga
     2281 ggcccgggcc gacatcccag cgcaggagga gtcgcgatga ctcttcgcat cggtacccgt
     2341 cccagcgcgc tcgccgtagc acaggcgggc accatcgccg accggctgag ttccgccggg
     2401 catccggccg agctggtcac ggtgaccacg tcaggcgacc gctcgggcgc acccgtcgcg
     2461 gagctcggcg tcggggtgtt cgtctcggcg ctgcgcgagg cgctggtgcg tggcgaggtc
     2521 gacgtcgccg tgcactccta caaagacctg ccgaccgcgc ccgatccgcg gctgcacatg
     2581 gcggccgtgc cacagcggga agacccccgc gatgcgctgg tcgcccgcga cgggatggtg
     2641 ctcggcgagc tccccccggg ggcgacggtg ggcaccggtt caccacgccg ccgagcgcag
     2701 ctcgaggcgc tcgggctcgg cctcgacgtc gtcccgatcc ggggcaatgt cgacacgcgc
     2761 atcggcaagg tccgctccgg cgagctcgac gccgtggtcg tggctgcggc cgggctgcgc
     2821 aggctgggac gcatcgacga ggccaccgag ctgctcgacc ccctgcagat gttgcccgcg
     2881 cccgcccagg gtgcgctggc catcgagtgc agggtcgacg acctggagct gggtcgcgcg
     2941 ctctccctgg cgctcgacga cgagcacacc cgggccgccg tcatcgcgga gcgcgcggtg
     3001 ctggccacgc tggaagccgg ctgtcacgcg cccgtcggcg cgctggccga tgtggtgtcc
     3061 gactgggaac cgatatccgg cgacgccggg ggggacgaca acggacgcgt ggtcgaccgc
     3121 gtgtcgttgc gcgcggtggt cggcacgacc gacggagcgc ttctgcgcgc ctccgtcacc
     3181 ggaaacttgg acgacgccga gaagctcgga tcggccttgg ccgcagagct gctcgacatg
     3241 gggggcctcg ctgccctcgg agaaccgctg gatcgggaga tctgatgaac cgagcaccga
     3301 gtaccccggg acgggtcgcc ttcgtgggca gtggccccgg cgaccccggc ctgctgaccg
     3361 tccgcgcgcg ggaggcgatc gtgaacgcgc cgctggtcgt caccgatccg gacgttcccg
     3421 aggtgatact tgcgctggtc gccgacggag ccgaggtgcg gcccgcggtc ggccagcccg
     3481 ctgatgtcgc gcgtgacctg gtcgcggagg cccgctccgg ccgcaccgtc acgaggctcg
     3541 tcagcggcga cccgctgacc gtcgactcgg tcgtgcagga ggccatcgcg gtggccgccg
     3601 ccgatgtcgc gttcgacgtc gtgcccgggg tcccggccgg cacggccgtc cccgcctacg
     3661 ccggcgtgcc gctgggctcg gcgcacatcg aggcagacgt ccgggcgggc gttgactggg
     3721 aacggctcgc cgcagcgccg ggaacgctcg ttctgcacgc ctccccgtcg cacctgtcgg
     3781 aggtggtgag cgcgctcacc gagcacggtc tcgccgcgca gacgccggtg gccgtcaccg
     3841 cgagtggcac cgcgaccacg cagaagaccg tccaggcgac tctcgccacc atggcctcgg
     3901 aggccgccga actcgagggg ccgctggtga tcaccgtcgg cgccgaggtg gggctgcgcg
     3961 ccgagctgtc ctggtgggag tcgcgcgcgc tgtacggctg gcgcgtgctg gtgccccgca
     4021 cgaaggacca ggcgggtgcc atgagcgacc ggctggccgt gcacggcgcc accgccgagg
     4081 aggtgccgac gatcgctgtc gagccgccgc gctcgcccac gcagatggag cgggccgtca
     4141 agggcctggt cgacggccgt taccagtggg tggtcttcac ctccaccaac gccgtgcgtg
     4201 cggtgtggga gaagttcgcc gagttcgggc tcgacgcccg tgccttctcc ggcgtcaaga
     4261 tcgcctgtgt cggcacggcg accgcggaga aggtgcgcag cttcggcatc gtgccggagc
     4321 tggtgccgtc ggccgaggag tcgcaggagt cctcgtccga ggggctgctg tcgatcttcc
     4381 cgccttatga tgacgtgctc gacccggtcg accgcgtgct gctgccgcgc gccgacatcg
     4441 ccaccgagac gctcgctgcc ggcctacgtg accgtggctg ggagatcgac gacgtcaccg
     4501 cgtaccgcac ggtgcgcgcg gcgccgccgc ccgccccgat ccgcgaggcg atcaagaccg
     4561 gcgggttcga cgccgtgtgc ttcacgtcgt cgtccacggt gcgcaacctg gtcgggatcg
     4621 cgggcaagcc gcacgcccgc acgatcgtgg cgtgcatcgg cccgcagacg gccgagacag
     4681 ctcgcgagtt cggcctgcgg gttgacgtgc agcccgacga agctcgcgtg cccgcactgg
     4741 tcgacgcatt ggcggcgcac gccgcgcgac tccgggcgga gggcgccctc ccgcctccga
     4801 agaaggcgaa ggcccgccgc cgctgatccg ggccgacccg acgttgcaga gaagccacgt
     4861 tcacgcaacc agcttgcgtg gacgtggctt cctgcaatcc gggggtgcgg acgggcgggt
     4921 gtgggcggcg ccgacctgct gatcatgggc aagtcccgtc ggacgtcatg ccgtgcaggg
     4981 agtcgtagtg tgggcgtatg gcgtttcccc tgcatcggcc gcggcggctg cggtccacgc
     5041 ccgccctgcg ccggctcgtc gccgagaccg acctccggcc ccggcacctc gtgcttccga
     5101 tgttcgtgaa ggagggtgcg ggcgagccgg tgccgatcgc gtcgatgccc ggcgtggtcc
     5161 agcacacccg cgactcgctg cggaaggcgg ccaccgaggc cgtcgccgcg ggtgtgggtg
     5221 gcctgatgct cttcggtatc cccgagcggc gtgacgccgc gggttccgcc ggcgtggacc
     5281 ccgacggagt gctgaacgtc gcattgcgtg acgtcagcgc cgaggtcggg gatgccaccg
     5341 tgctgatggc cgacacgtgt ctcgacgagt tcaccgacca cggccactgc ggtgtgctcg
     5401 acgcggacgg ccgggtggac aacgacgcca cgctcgccgt ctacgccgag atggctgttg
     5461 cccaggccga ggccggggcg cacctggtcg ggcccagcgg gatgatggac ggccaggtcg
     5521 gcgtgatccg cgaggcgctc gacgccgcag gccacctcga caccggcatc ctcgcgtaca
     5581 cggccaagta ctcctcggcg ttctacggac cgttccgcga ggccgtcgag tcgcagctgc
     5641 agggtgaccg caagacctac cagcaggacg gcgccaacgt gcgggaggcc ctgcgcgagc
     5701 tcgcgctcga cctcgaggag ggcgcagacc tggtgatggt caagcccgcg ctggcatacc
     5761 tcgacgtgct gcgcgtcgtg gccgaggccg ccgacgttcc ggtggcggcc tatcaggtgt
     5821 ccggcgagta cgccatggtg gaggcggcgg cggccaatgg atggctggac cgcgaccgca
     5881 cggtcctcga gacgctcacc gcgagccgcc gcgcgggtgc ggacatcgtc ctcacctact
     5941 gggccaccga ggtcgccggc aggctcacga tcgagtagat cgtggtgtcg tgaccggttc
     6001 gcaacactcg tcgtcgccgc ggatgatccg aaatgcgccg aatcggcgat acgatcgcgc
     6061 ccccgcttga tcatgtggag gctcgcgatg acgtacgacc ccgggaaccc cggcgagcag
     6121 ccccagccgc ccgtcggcca gggctggtcc ggcggtcagc agccgccctt tccggggggc
     6181 tatggccagc agccgcctcc cggctggggt cctccccccg gtcagccggg ctacggcggc
     6241 ggcccgacgg gcctcgggcc gaccgggagc ccacagatgc aggcaaaggg cttcttcacc
     6301 gcgctgttcg acttcgggtt caacagcttc gcgacgccct cggtggtgaa ggtcatctac
     6361 gtcatcggca tggtgatcct cggcctggct tacgtcggct tcgtcatcgc cgggttcacc
     6421 cagggcgccg ggcaggggct gctcgtgctc atcctcgggg gagtcgtcgc tctgttctac
     6481 ctgacgctgt tccggatcac cctcgagttc tactacgccg tcgtccgcat gtcggaggac
     6541 atccaccacc ggcgttgaga gttgcgtgga cagccaatca ggcgagccct acggtagccg
     6601 cgtgagtaca ccgtccgatc cgcagcgcgg ggaccagcag cctgacgacg gtccccgcgt
     6661 cggtgacggc tcggacggca cgcaggagcc gccgtccggc caacagccgg gcgaggcggg
     6721 acagccgggc gaggcgggac agccgggcca gtacggtcag cccggtccgt acggccagcc
     6781 tggccagtac ggtcagcccg gccagtacgg ccagcccggt ccgtacggcc agcccggcca
     6841 gtacggccag cccggtccgt acggtcagcc cggccagtac ggtcagcccg gtccgtacgg
     6901 tcagcccggc cagtacggtc agcccggcca gtacggatca gcgccctacg gccagcccgg
     6961 gccctatgg
//
LOCUS       DASRSC010000347         6279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4728349,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000347 DASRSC010000000
VERSION     DASRSC010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4728349"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VFE39_16850"
     CDS             complement(<1..411)
                     /locus_tag="VFE39_16850"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6260307.1"
                     /translation="MSTGDERASRTGVQAVDRAVAILRCFDARRPDLGISEIARMTGL
                     STSTTHRLLVAMQSNRLVRQTSDRRYGLGPLLVQLARSGAVPTTLRDAALPFMRALRD
                     EIDETVAVHELLPTGERAVVDQVESRQELRRTYTD"
     gene            528..1742
                     /locus_tag="VFE39_16855"
     CDS             528..1742
                     /locus_tag="VFE39_16855"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HET6260308.1"
                     /translation="MLPLDGFRVLDLTRFLSGPYCTMVLAELGAEVIKVEQPITGDDS
                     RRLAPKVNGESYPSAMPNRSKRSISIDLKSDRGRELFLRLARDADLVIENFRPGVMRK
                     LGIDYDAVRAARPDVLYCSISGFGQTGPYRDRPGFDIMAQGAIGLMRMTGEPGGRPAK
                     VGIAINDIAAGATAIYSVLAAQIKRARTGTGQHIDISLVDAGLAWTVWESGAYFATGE
                     VPQGTGTRHRRSTPYQAYRTADGYVTIGANNDRLWKRLVSDVLDRPDWLTDPRFVTLA
                     DRMDHIDELEVEIERITTMRATEEWIKLLERAGVPGGPVLTYDEALADPHVQASGMLV
                     DLEHPLIGAMRTIGPPTKFSDLDFRVRGPAPWLGQHTAQVLRDSGVDDAELGELFASG
                     VAYDAHPDLERS"
     gene            1745..2527
                     /locus_tag="VFE39_16860"
     CDS             1745..2527
                     /locus_tag="VFE39_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HET6260309.1"
                     /translation="MSDHILVERADAVATVVLNRPQTHNAITVEMYRELPAVLGGLDD
                     DPAVKAIVIRGSGQKAFASGADISEFERERGDAERARSYNEHVAAAERALEAMAKPTI
                     AMVHGYCIGGGAGLALACDLRFADDRSRFAITPAKLGLVYSLESTKRVVDLVGPSRAT
                     WILVSGQQIPAGRALQLGLFDEVTGTDDLARLTYDFAELVTTRAQFSVRQGKQMVRRV
                     VAGQRSDDEETTGIRNSSFDTADYAEGVRSFLEKRPPRFTWS"
     gene            2548..2997
                     /locus_tag="VFE39_16865"
     CDS             2548..2997
                     /locus_tag="VFE39_16865"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET6260310.1"
                     /translation="MTATQDLHPAVAHAAAAAAEARPEFGEFFLARFLGLDISYDDQA
                     RTCTVVLPYAAHLCNPQGSMHGGVITTAMDISMGHLCHRFLSTAVTIEMNLRFFRPLT
                     GTGRCHAAVLRPGRRIVHLEARLTDEQDRLVAFAAGSWHRLDAEPST"
     gene            3155..4003
                     /locus_tag="VFE39_16870"
     CDS             3155..4003
                     /locus_tag="VFE39_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010696110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET6260311.1"
                     /translation="MRLASIRTGTTEVPAIVDPVRGVVPVPALFPGFDGDLLAVITQG
                     LHGELAKLAETAPDELFVPRETVAFGPPYRHPRLIWGIGLNYVDHAADLSEQVPDEPA
                     SFMKGDHTVIGPGEPIPIPRQSARTTAEAELGLVIGRYCRNVSEAEAPDHVLGVTTIL
                     DQTAEDILERNPRFLTRSKNFPGFFSFGPEIVPLADLPNGVRDLSALEVSTVLNGAVH
                     RSNTVARMRYSPQFLVSFHSAVMPLQPGDIIATGTPGAAHVRPGDTVQCRIPGVGVLA
                     NPVVQG"
     gene            complement(4289..5401)
                     /locus_tag="VFE39_16875"
     CDS             complement(4289..5401)
                     /locus_tag="VFE39_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HET6260312.1"
                     /translation="MDVEGQVELVFSGEVARTGNHVNLSAVRTEGEHLWLAGDETATI
                     ERLVLDSPSVPTRGGSERSFALADFVDLPGPPDEEADIEGIARAGGWLWAVGSHSLAR
                     KQIKPHHSEVKAVKRLAKIKRQANRYVMARLAVEPGADGRPEPVRVAADGRHSALVGG
                     AGSENLADLLGDDEHLAPFLAIPSKDNGLDVEGLAAHGESLYVGLRGPVLRGWAVVLE
                     LLPVPDPTDPARLALGAFGDGTRYRKHFLDLGGLGVRDLCPDGNDLLVLAGPSMALSG
                     PVRVYRWHHAATAVTGRVVRGEEISLVTALPHGDGVDHPEGIALLEPGDPAGARLLVV
                     YDSPSLARRPTSHSVLADRVVIDSAQPRAQWEANPT"
     gene            5641..6045
                     /locus_tag="VFE39_16880"
     CDS             5641..6045
                     /locus_tag="VFE39_16880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6260313.1"
                     /translation="MLRCLIVDDSPRFLDAARGLLEREGIAVVGVASTGPEALERAGE
                     LLPDVTLVDIDLGGESGFDVVRRLHREAHLAPSRMILISSHAEEDYADLIAASPAAGF
                     LSKTRMSADAVRDLLQLRGDGEPDPVSGPRGS"
BASE COUNT          966 a         2269 c         2121 g          923 t
ORIGIN      
        1 atcggtgtag gtccgccgca gctcctggcg gctctcgacc tggtcgacga ccgcccgctc
       61 cccggtcggg agcagttcgt gcacggccac cgtctcgtcg atctcatcgc gcagcgcgcg
      121 catgaacggc agcgcggcgt cccgcagcgt cgtcggcacc gcgccgctgc gggcgagctg
      181 caccagcaac gggccgagcc cgtagcggcg atcgctggtc tggcggacga gccggttgct
      241 ctgcatggcg accagcagtc ggtgcgtcgt gctcgtggag agcccggtca tccgggcaat
      301 ctcgctgatc ccgaggtcgg gccggcgagc gtcgaagcag cgcaggatcg ccaccgcccg
      361 gtcgacggct tgcacgccgg tgcgcgacgc ccgctcgtcc cccgtgctca cgcctcgccc
      421 ccgctctctc ggaggtcggc gtcctccgtt cccttgacct gcgtcacatc ggatcgtagg
      481 cttcccgtaa cacgggacgc aatcccgcaa tacgggaaag gcacggaatg ctcccactcg
      541 acggtttccg ggtcctcgat ctgacccggt tcctctccgg gccgtactgc acgatggtgc
      601 tggccgagct cggcgctgag gtgatcaagg tcgagcagcc gatcaccggg gacgactcac
      661 gcaggctggc tccgaaggtg aacggagaga gctatccgtc cgcgatgccg aaccggagca
      721 agcgcagcat ctcgatcgac ctcaagagcg accgcggccg cgagctcttc ctccggctcg
      781 cccgcgatgc cgatctcgtg atcgagaact tccgcccagg tgtgatgcgc aagctcggca
      841 tcgactacga cgcggtgcgc gcggcccgcc cggatgtcct gtactgctcc atcagcggct
      901 tcgggcagac cgggccgtac cgggaccgtc cgggcttcga catcatggcg cagggcgcca
      961 tcggcctgat gcggatgacc ggtgagcccg gcggccgccc tgcgaaggtc ggcatcgcga
     1021 tcaacgacat cgccgcgggc gccaccgcga tctactcggt gctggccgcc cagatcaagc
     1081 gggcgcgcac cgggacgggc cagcacatcg acatctcgct cgtcgacgcc ggcctggcgt
     1141 ggacggtgtg ggagtccggc gcctacttcg ccaccggcga ggtgccgcag ggcaccggca
     1201 cccggcaccg tcggtccacg ccgtaccagg cgtaccgcac cgccgacggg tacgtcacga
     1261 tcggcgcgaa caacgatcgg ctgtggaagc ggctggtgag cgatgtgctc gaccggcccg
     1321 actggctgac ggacccccgc ttcgtcactc tcgccgatcg gatggaccac atcgacgagc
     1381 tcgaggtgga gatcgagcgc atcacgacga tgcgtgcgac cgaggagtgg atcaagctcc
     1441 tggagcgggc cggcgtgccc ggtggtccgg tgctgaccta tgacgaggcg ctcgccgatc
     1501 cgcacgtgca ggccagcggg atgctcgtcg acctggagca cccgctgatc ggggcgatgc
     1561 gcacgatcgg cccgcccacg aagttctccg acctggactt ccgggtccgc gggccggcgc
     1621 cgtggctggg ccagcacacc gcccaggtcc tgcgcgactc cggtgtggac gatgcggagc
     1681 tgggtgagct gttcgccagc ggtgtggcct atgacgccca cccggacctc gagaggagct
     1741 gagcgtgtcc gaccacatcc tggtagagcg cgcagacgcc gtggccacgg tcgtgctcaa
     1801 ccggccgcag acccacaacg cgatcaccgt cgagatgtac cgggagctac cggccgtgct
     1861 cggcggcctc gatgacgacc ccgccgtgaa ggccattgtg atccgcggct ccgggcagaa
     1921 ggcgttcgcc tccggtgccg acatcagcga gttcgagcgc gagcgcggcg acgccgagcg
     1981 ggcccgcagc tacaacgagc acgtcgccgc cgcggagcgg gcgctcgagg cgatggcgaa
     2041 gccgacgatc gcgatggtgc acggctactg catcggcggt ggcgccggcc tggcactggc
     2101 ctgtgacctg cgcttcgccg acgaccgcag ccgcttcgcc atcaccccgg ccaagctcgg
     2161 gctggtctac agcctcgagt ccaccaagcg ggtcgtcgac ctcgtcgggc cgtcgcgcgc
     2221 cacctggatc ctggtctccg gtcaacagat ccccgccggc cgggccctgc agctcggcct
     2281 gttcgacgag gtgaccggca cagacgatct cgcccggctc acctacgact tcgccgagct
     2341 tgtgacgacg cgggcgcagt tcagcgtccg ccagggcaag cagatggtgc gcagggtcgt
     2401 cgccgggcag cgcagcgacg acgaggagac cacaggcatc cgcaactcct ccttcgacac
     2461 cgccgactac gccgaggggg tgcgctcgtt cctcgagaag cgccccccgc ggttcacctg
     2521 gtcgtgactc cgccgatgac tccgccgatg accgccacgc aggacttgca ccccgccgtc
     2581 gcgcacgccg cggccgccgc ggcggaggcc cggcccgagt tcggcgagtt cttcctcgcc
     2641 cgcttcctcg gcctcgacat cagctacgac gaccaggccc gcacctgcac cgtcgtcctg
     2701 ccctacgccg cgcacctgtg caacccgcag ggctccatgc acggcggggt gatcaccacg
     2761 gccatggaca tctccatggg ccacctctgc caccgcttcc tgtccaccgc cgtgacgatc
     2821 gagatgaacc tgcgtttctt ccggccgctc accggcaccg gccgctgcca cgcggcggtg
     2881 ctgcggccgg gccgccggat cgtgcacctc gaggcccgac tcaccgacga gcaggaccgg
     2941 ctggtggcgt tcgcagccgg ctcctggcac cgcctggacg ccgagcccag cacgtagacc
     3001 actcacccgg caacggagcc ggggaaggca cagcgccgct ccgccgacgg ggcagacccg
     3061 gccgagacca ccgagaccac agggcatcgg tgagcaggcg tcccggtcgc cggaccaacc
     3121 actgccgccg acccgcgatt cgaactggag aaccatgcga ctggccagca tccgcaccgg
     3181 caccaccgag gtccccgcga tcgtggaccc ggtccgcggt gtcgtccccg tcccggcgct
     3241 cttccccggg ttcgacggtg atctgctcgc cgtcatcacg cagggcctgc acggcgagct
     3301 ggcgaagctc gccgaaaccg cgcccgacga actgttcgtc ccccgggaga ccgtcgcctt
     3361 cggccctccg taccggcacc cgcggctgat ctggggaatc ggcctcaact acgtcgacca
     3421 tgcggccgac ctctccgagc aggtgcccga cgagcccgcc tccttcatga agggcgacca
     3481 caccgtcatc ggcccgggag agcccatccc gataccgcgc cagagcgccc ggaccacagc
     3541 agaggcggag ctggggctcg tcatcggccg gtactgccgc aacgtgtccg aggcagaggc
     3601 gcccgaccat gttctcggtg tcacgacgat cctcgaccag accgcggaag acattctcga
     3661 gcgcaacccg cgcttcctca cccgctccaa gaacttcccg gggttcttct cgttcggccc
     3721 agagatcgtg ccgctagccg acctgccgaa tggggtgcgt gacctgtcgg cgctggaggt
     3781 gtcgaccgtg ctgaatggcg cggtgcaccg ctccaacacg gtggccagga tgcggtactc
     3841 gccgcagttc ctggtcagct tccacagcgc ggtcatgccg ctgcaacccg gtgacatcat
     3901 cgcgacgggc acaccgggcg cggcgcatgt ccgtccgggc gacacggtcc aatgccgcat
     3961 cccgggcgtg ggtgtgctgg ccaaccccgt cgtgcagggt tagcggacgc gccggcccag
     4021 tcgagcaggc gcgggcggcg ctacggcttg ccggtcaggg cggagaaggc ggcggcgacg
     4081 acgagacccc ccaccacgat cgcgatcagg aagcccgaaa ggccaaaaga ctcggaaatt
     4141 gaagcaatag cctgaagtgt gccacccatt gtcccgtcgt cctctctgga ttcccacctg
     4201 cggcgcgggg cgatacgtga accatgctcg ctcggatctc cgggagcgat agagcgagcc
     4261 ccacagtccg gagccacaac accccagctc aggtggggtt tgcctcccac tgagcccgtg
     4321 gttgcgcgct atcgattacc acgcgatcag caagcaccga gtgcgaggtg ggcctgcggg
     4381 ccagggacgg gctgtcgtag acgacgagca gccgcgcacc cgcggggtcg cccggctcga
     4441 gcagcgcgat gccctcaggg tggtcgacgc cgtcgccatg gggcagtgcc gtcacaagac
     4501 tgatctcttc gccgcgcacc acccgcccgg tcacggccgt cgccgcgtga tgccagcggt
     4561 acacccgcac cgggccggag agcgccatcg acggccctgc cagtacgagc agatcgttgc
     4621 cgtctgggca caggtcccgt acaccgaggc cgccgaggtc gaggaagtgt ttgcggtagc
     4681 gggtgccgtc gccgaatgcg ccgagcgcca gccgggcggg atcggtggga tcggggaccg
     4741 gcagcagctc gagcaccaca gcccacccgc gcagtaccgg gccgcgcagg ccgacgtaca
     4801 gcgactcgcc gtgcgcggcg aggccctcga cgtcgagccc attgtccttg gacgggatcg
     4861 cgaggaaggg cgcgaggtgc tcgtcgtcgc ccagcagatc ggcgaggttc tcggagccgg
     4921 cgccgccgac gagcgccgag tggcggccgt cggcagccac ccgcaccggc tcgggtctgc
     4981 cgtccgcacc aggttcgacg gcgagccggg ccatcacata gcggttggcc tgtcgcttga
     5041 tcttggcaag ccgcttgacc gccttcacct cgctgtggtg tggcttgatc tgtttgcggg
     5101 ccaagctgtg tgatccgacg gcccagagcc agcccccggc ccgggcgatg ccctcgatgt
     5161 cggcctcctc gtcaggtggc ccgggcaggt cgacgaagtc ggccagcgcg aaactgcgct
     5221 cgctcccacc gcgggtgggc acggacgggg agtcgagtac gagccgctcg atcgttgccg
     5281 tctcgtctcc ggcgagccag aggtgctcgc cctctgtccg caccgccgag agattgacgt
     5341 gattgccggt gcgcgccacc tcaccgctga acaccaactc gacctgaccc tcgacgtcca
     5401 cgagtcccat tgtcgtgggc actgtgtcgt ggacaacact gtgtcgtggg caacactgtg
     5461 tcgtggccat cgccgtaggc tcaggtcagc gcccgtgacg gcccggccct gtgccagctc
     5521 cgtcttcgaa cagacctgca cccggtcgtg gtcggccgcg cgccgcacgg cggctgcgcc
     5581 ccctgcacgt ggctctgctg ctggcatgcg cgctcagcag cgacgtgcga ttcttgagcc
     5641 gtgctgcgct gcctcatcgt cgacgacagc cctcggttcc tcgatgccgc gcgcggcttg
     5701 ctggagcggg agggcatcgc ggtcgtgggt gtggcgtcga ccggccctga ggcgctcgag
     5761 cgcgccgggg agctcctgcc cgacgtcacg ctggtggaca tcgacctcgg cggagagagc
     5821 ggcttcgacg tggtgcggcg actccaccgc gaagcgcacc tggccccgtc acggatgatc
     5881 ctgatatcca gccacgcaga ggaggactac gccgatctga tcgcggcgag cccggcggcc
     5941 gggttcctgt ccaaaaccag gatgtccgcc gacgcagtcc gggacctgct ccagctccgg
     6001 ggtgacggcg agcccgaccc tgtcagcggg cctcgaggaa gttgagcaca gcgaggacga
     6061 gccgtacaga ttcgggttcc atcgtcctgg ccagcactat cggctgatct cacattctgt
     6121 caggtcacta cgctgcggag gtgtcggcga agcgttcacg ggcgcgagcg caaggccagc
     6181 aaccgcgtga aatccgaccg gaagatgagc tgtgggcggg ctggcccatc acatgatcgt
     6241 cgcgagcggc ccgctcgcgt tggtcgtggg cgccgcctt
//
LOCUS       DASRSC010000348         5341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_636306,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000348 DASRSC010000000
VERSION     DASRSC010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_636306"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..804
                     /gene="modA"
                     /locus_tag="VFE39_16885"
     CDS             25..804
                     /gene="modA"
                     /locus_tag="VFE39_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6260314.1"
                     /translation="MRMAAAAAAGLLVLAGCGGGTTNGAAAGGSSAAPEDRTLTVFAA
                     ASLTESFGELEKQFETDNPGVDVVLNYGGSSDLAQQIVNGAPADVFASASDSTMKTVS
                     DAGLVDGTPSVFAKNVLQIAAVPGNPKGIMSFADLAKPDLKVVVCAPQVPCGAAAGKI
                     ETATGVTLRPVSEEPDVKSVLGKVTSGDADAGLVYVTDVASAGDKVQGVDFPEAAQAP
                     TTYPIAVITDAPQTELGRSFVNMVTGEPGQKVLGAAGFEAP"
     gene            810..1637
                     /locus_tag="VFE39_16890"
     CDS             810..1637
                     /locus_tag="VFE39_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6260315.1"
                     /translation="MPSDASARRSDEHETGVGLPRLLWVPATLAFALIALPVLGLVLR
                     ADWPRVPALLLSESALDALRLSLLTAGISTALCIVLGGPLAVVLARGRLRALRLLRSV
                     VLLPLVLPPVVGGLALLFLLGRTGLLGRGLDVAFGVTIPFTTAAVVLAQTFVALPFLV
                     VSLEGSLRTAGRRYEAVAATLGASPGLAFRRVTVPLVLPGLASGTVLAFARCLGEFGA
                     TIAFAGSLQGTTRTLPLLVYLERETDVDAAVALSLLLVVVAVAVIAVARPRTVEGAR"
     gene            1634..2737
                     /locus_tag="VFE39_16895"
     CDS             1634..2737
                     /locus_tag="VFE39_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6260316.1"
                     /translation="MSRLVARVVVRRAAFTLDVALSAEPGEVLAVLGPNGAGKSTLLG
                     VLAGLLRPDAGHVRVGDVTVTDPGSGVHVPPHQRGVGLLAQQPLLFPHLSAVGNVAFG
                     PRAHGVPRREAERRARELLTAVDAAELADRRPAGMSGGQQQRVALARALAPGPGLLLL
                     DEPLAALDVDATPAMRSLLRRVIRDAKQTAVLVTHSALDALVLADRVVVLTGGRVVEQ
                     GSTRDVLGRPRSPFTARIAGLDLVPGTACPEGLRTADGMVVSGLHNGDSVGPADGEPA
                     VAVFPPSAVAVFAERPHGSPRNVLHVRLAAIEPHGDVVRLRAGAVSGGPEWMDGLAAD
                     VTPAAVADLAAEPGAELWFAVKATEVGIHAASS"
     gene            2855..4024
                     /locus_tag="VFE39_16900"
     CDS             2855..4024
                     /locus_tag="VFE39_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET6260317.1"
                     /translation="MTRLLLRIAPAVAALLLLVALGTPATAAAQDELSQRTDYVVHTR
                     TSAEAGAAADVLEVRPTVIFDQVSAGFAASLTRAQVDRLHGLPGVLAVEEDRRITAFE
                     PRAAPGRILREGTQLDPPNWGLDRIDQRALPLEAGYTTRATGAGVTIYVLDTGIDTTH
                     PQFGGRASAGANTVDDVAGDCDGHGTVVAGIAASRDYGVAKEAQLRSVKVLDCSGGGT
                     LSSLLAGIDYVARNQQGPSIAVMSWSYGPSDVLTSAVTELVKRGVFVASSAGNTGSND
                     CTAAPRAADGVMVVANSTIDDHRAETSSTGSCVDLYAPGTSIVSTVPGGTSSYTGSSM
                     AAPHAAGVAALYKQTFGDAPSRTVEQWIVGHATQRVIAGGGEGGTPNLLLCTGGL"
     gene            complement(4045..>5341)
                     /locus_tag="VFE39_16905"
     CDS             complement(4045..>5341)
                     /locus_tag="VFE39_16905"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HET6260318.1"
                     /translation="YRSARRAFDEGWRDTTPGERMTALLAMADTLEAHGEELVEIEAR
                     DTGKPTGLTLSEEIGPMADQLRFFAGAARQLEGRATGEYMAGHSSSIRREPLGVIGSI
                     TPWNYPMMMAIWKIGPALAAGNTMVLKPSDTTPASTVRLGELFADVLPPGVLNVVCGD
                     RSTGVAMSENPIPAMLAITGSIPAGKSVAAAAASRVTRVHLELGGNAPVIVFDDADVA
                     AAAADIAVAGFFNAGQDCTAATRVIAHADVADALAEALAAEAGGVLLSRDEQPGAEDL
                     FIPPLNNPDQLAKVSGLVERVPSHARVLTGGAPADGPGWFYPPTVVAGLAQDDEIVQT
                     EVFGPVITVQTFTDEDEAVAYANGTPYGLASSVWTRDHSRTIRLSRALDFGCVWVNCH
                     IPLVAEMPHGGFKESGYGKDLSGYSLEDYTRIKHVMSKY"
BASE COUNT          664 a         1853 c         2023 g          801 t
ORIGIN      
        1 gactccgggc ggaacgtcat gaggatgcgg atggcggcgg ccgcggcggc cgggctgctg
       61 gtcctggccg ggtgcggtgg gggaacgacg aacggtgcgg ccgccggggg gtcgtccgcc
      121 gcaccggagg atcgcaccct caccgtcttc gccgcggcgt cgctgaccga gtcgttcggc
      181 gagctggaga agcagttcga gaccgacaac ccgggcgtgg acgtcgtcct caactacggg
      241 ggctcgtccg acctcgcgca gcagatcgtg aacggtgcgc ctgccgacgt gttcgcatcg
      301 gcgagcgact ccacgatgaa gacggtgtcc gatgccgggc tcgtcgacgg aacgccctcc
      361 gtgttcgcca agaacgtgct gcagatcgcc gccgtgccgg gcaacccgaa ggggatcatg
      421 tccttcgccg acctcgcgaa gcccgacctc aaggtcgtcg tctgcgcacc gcaggtgccc
      481 tgcggcgcag ccgcggggaa gatcgagacc gcgaccggcg tgaccctgcg tccggtcagc
      541 gaggagcccg acgtgaagtc ggtgctgggc aaggtcacga gcggcgacgc cgacgccggc
      601 ctcgtctacg tcaccgacgt ggcgtcggca ggcgacaagg tgcagggcgt cgacttcccg
      661 gaggcagcgc aggcccccac cacctacccc attgccgtga tcacggacgc gccgcagacc
      721 gagctgggcc ggtccttcgt gaacatggtg acgggcgagc cgggccagaa ggtgctgggg
      781 gcggccggct tcgaggcgcc gtgaccgcag tgcccagtga cgcctctgcc cggcggtcgg
      841 acgagcacga gaccggcgtg gggttgcccc gcctgctctg ggtgcccgcc acgctggcct
      901 tcgcgctgat cgcgctgccc gtgctcggac tcgtcctccg ggccgactgg ccacgcgtcc
      961 cggcgctgct gctcagcgag tcggcgctcg acgcgttgcg actctcgctc ctgacggcgg
     1021 ggatctcgac ggccctgtgc atcgtcctcg gtgggccgct cgcggtggtg ctggcccggg
     1081 gcaggctgcg cgcactgcga ctgctccgct cggtggtgct gctgccgctg gtgctgccgc
     1141 cggtcgtggg cggactggca ttgctgttcc tgctcgggcg caccgggctg ctcggccgtg
     1201 ggctggacgt cgcgttcggc gtgacgatcc cgttcaccac cgctgcggtg gtgctggcgc
     1261 agacgttcgt cgcgctgccg ttcctcgtcg tcagccttga ggggtcgctg cggacggctg
     1321 gccggcgcta cgaagcggtg gcggcaacgc tcggagcatc gccgggcctg gccttccggc
     1381 gtgtcacggt gccgctggtg ttgcctggcc tcgcgtcggg cacggtactg gcgttcgcgc
     1441 gctgcctggg cgagttcggc gcgacgatcg ccttcgccgg cagcctgcag ggcacgacgc
     1501 gcacgctgcc gctcttggtc tacctggagc gggagaccga cgtggacgcc gcagtggcgc
     1561 tgtcgctgct gctggtggtg gtggctgtcg ctgtgatcgc ggtggcgcgg ccgcgcaccg
     1621 tcgagggcgc acggtgagtc ggctcgtcgc gcgcgtggtg gtgcggcggg cggcgttcac
     1681 cctcgacgtg gcgctgtcgg cggagccggg cgaggtgctg gccgtgctcg ggccgaacgg
     1741 ggcgggcaag tccactctgc tcggggtgtt ggccgggctg ctgcgaccgg acgcggggca
     1801 cgtccgcgtg ggagacgtga cggtgaccga ccccggatcc ggcgtgcacg tgcccccgca
     1861 tcagcgcggc gtggggttgc tggcgcagca gcccctgctc ttcccgcacc tgtcggccgt
     1921 cgggaacgtt gccttcggtc cgcgggccca cggcgtgccg cgccgggagg cggagcggcg
     1981 ggcccgcgag ctactgactg ccgtcgacgc ggcggagctg gccgaccggc ggccggccgg
     2041 catgtccggg ggccagcagc aacgggtggc cctggctcgt gcgctggcgc cggggcccgg
     2101 gctcctgctg ctcgacgagc ccttggcggc gctggacgtc gacgcgaccc cggccatgcg
     2161 ctcgctgctg cgccgcgtga tccgcgacgc gaagcagacc gccgtgctgg tcacccactc
     2221 ggcgctcgat gcgctcgtgc tggccgatcg ggtcgtggtg ctgaccgggg gccgggtggt
     2281 ggagcagggc tccacgcgag acgtgctcgg tcgaccgcgc agcccgttca ccgcgcggat
     2341 cgccgggctg gacctcgtgc cgggcacggc gtgtcccgaa gggctgcgga cggcggacgg
     2401 gatggtcgtg tcgggacttc acaacggcga cagcgtcggt cctgccgacg gtgaacccgc
     2461 ggtcgccgtg ttcccgccct cggcggtcgc ggtcttcgcc gaacggcctc acggcagtcc
     2521 gcgcaacgtg ctgcacgtgc ggctggcggc gatcgaaccg cacggtgacg tggtgcggct
     2581 gcgggccggg gccgtgtcgg gaggacccga gtggatggac gggctcgctg ccgacgtgac
     2641 gccggcggcg gtggccgatc tcgctgcaga gccgggggcg gagctgtggt tcgcggtcaa
     2701 ggccaccgag gtcgggatcc acgccgcgag cagctgacgt ccacaagggc ccttctggac
     2761 ggggggttcg attacaccca cgtacctcac ctgtcacccc tgccccaatc gtgtctctgt
     2821 ccccactcgt ttccgggccg taccgtcgct gcacgtgaca cgcctgctcc tgcggatcgc
     2881 tccggcggtg gcagccctcc tgctcctcgt tgccctcggg actccggcga cggccgcggc
     2941 gcaggacgag ctgtcccagc ggaccgacta cgtggtgcac acccgcacga gcgccgaggc
     3001 gggggcggca gccgatgtgc tcgaggtacg accgacggtc attttcgacc aggtgtccgc
     3061 gggattcgcc gcgagcctca cccgggctca ggtcgaccgg ctgcacgggc tgcccggcgt
     3121 gctcgcggtc gaggaggacc ggcggatcac cgcgttcgag ccgcgtgccg ccccggggcg
     3181 gatcctgcgc gagggcaccc agctcgaccc gcccaactgg ggcctggacc gcatcgacca
     3241 gcgtgccctc ccgctcgagg ccggctacac gacgcgggcc acgggggcag gtgtgaccat
     3301 ctacgtgctg gacaccggca tcgacacgac gcacccccag ttcggcggca gggcgagcgc
     3361 gggggcgaac acggtcgacg acgtggcggg tgactgcgac ggtcacggca cggtggtggc
     3421 gggtatcgcc gcctcccggg actacggcgt ggccaaggag gcacagctcc ggtcggtcaa
     3481 ggtgctcgac tgcagtggtg gcggcaccct gtcgtcgctg ctggcgggca tcgactacgt
     3541 ggcgcgcaac cagcagggcc cgtccatcgc ggtgatgtcg tggagctacg ggccgtcgga
     3601 cgtgctgacg tcggcggtga cggagctcgt gaagaggggc gtcttcgtgg cctcgtccgc
     3661 gggtaatacc gggtcgaacg actgcaccgc cgcgccacgc gccgcggacg gcgtcatggt
     3721 cgtggcgaac tcgacgatcg acgaccaccg ggcggagacc tcgtccaccg gcagctgcgt
     3781 ggacctctat gcgccgggaa cgtccatcgt ctcgaccgtt cccggcggca caagctccta
     3841 cacgggctcc tcgatggcgg cgccgcacgc ggcgggagtg gcagcgttgt acaagcagac
     3901 gttcggcgac gcgcccagcc gcaccgtgga gcagtggatc gtgggtcacg cgacgcagcg
     3961 cgtcatcgcc ggcggtggcg agggtggcac cccgaatctc ctgctctgca cgggcggcct
     4021 gtgacgctca ggcgctgtac ctgctcagta cttgctcata acgtgcttga tccgcgtgta
     4081 gtcctccagc gagtagcccg acaggtcctt gccgtagccg gactccttga acccaccgtg
     4141 cggcatctcc gccaccaggg ggatgtggca gttgacccag acacagccga agtccagggc
     4201 gcgcgacagc cggatggtcc tggagtggtc gcgcgtccag acgctcgacg cgaggccgta
     4261 gggcgtgccg ttggcgtagg cgacggcctc gtcctcgtcg gtgaacgtct ggacggtgat
     4321 caccggtccg aagacctcgg tctgcacgat ctcgtcgtcc tgggcgagcc cggccaccac
     4381 ggtgggcggg tagaaccagc cgggaccgtc cgcgggggca ccgccggtga gcacccgtgc
     4441 gtgcgatggc acgcgctcga ccaggcccga caccttcgcc agctggtcgg ggttgttgag
     4501 cggggggatg aagaggtcct ccgctccggg ctgctcgtca cgggagagga ggacaccgcc
     4561 ggcctcggcg gccagcgcct ccgccagcgc gtcggccacg tcggcgtggg cgatgacgcg
     4621 ggtggcggcc gtgcagtcct ggccggcgtt gaagaacccg gcgaccgcga tgtcggcggc
     4681 ggccgctgcc acgtcggcgt cgtcgaacac gatcaccggc gcgttgccgc cgagctcgag
     4741 gtgcacgcga gtgacgcggg aggctgcggc ggcggccacc gacttcccgg ccgggatgct
     4801 gccggtgatg gccagcatcg ccgggatcgg gttctcgctc atcgcgacgc cggtggaccg
     4861 gtcgccgcac acgacgttga gcaccccggg cggcagcacg tcggcgaaca gctcaccgag
     4921 ccgcactgtg ctggccggcg tggtgtccga cggcttgagc accatcgtgt tccccgccgc
     4981 gagcgccggg ccgatcttcc agatcgccat catcatgggg tagttccacg gcgtgatgga
     5041 gccgatcacc ccgaggggtt cgcgccggat cgacgaggag tggccggcca tgtactcgcc
     5101 cgtcgcgcgg ccctcgagct ggcgggcggc gccggcgaag aaccggagct ggtcggccat
     5161 cgggccgatc tcctccgaca gcgtcagccc cgtcggcttg ccggtgtcgc gcgcctcgat
     5221 ctccacgagc tcctcgccgt gggcctcgag ggtgtcggcc atcgcgagca gcgcggtcat
     5281 ccgctcgccc ggcgtggtgt cgcgccagcc ctcgtcgaac gcgcgccgcg cactccggta
     5341 g
//
LOCUS       DASRSC010000349         1755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3424450,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000349 DASRSC010000000
VERSION     DASRSC010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3424450"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..384)
                     /locus_tag="VFE39_16910"
     CDS             complement(139..384)
                     /locus_tag="VFE39_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260319.1"
                     /translation="MTSNDELLLALRAAARALSSNRSIRDLDRRSRASSGELLYLPVV
                     YVRQRILAIRARWADPRVARNLAVTHGVHVRPILVDP"
     gene            902..1072
                     /locus_tag="VFE39_16915"
     CDS             902..1072
                     /locus_tag="VFE39_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260320.1"
                     /translation="MTERAHEVRGPLALGVAVGVALRALSDLTPAAAGVRDGRLKVLL
                     RASLALSTTREL"
     gene            1122..>1755
                     /locus_tag="VFE39_16920"
     CDS             1122..>1755
                     /locus_tag="VFE39_16920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_103557054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HET6260321.1"
                     /translation="MMTVEDWAEVRRLHRAEGLPIKEIVRQTGLARNTVRTALRSERA
                     PTYQRPSRGSVVDAYEPAIRVLLASWPRMPGPVIAQRIGWSYSEGPLKKRLALIRPEY
                     LGVDPVDRVAYQPGEITQCDLWFPDTKIPVGNGQRWMLPVLVMVLGYSRYLSATMLPS
                     RQAGDLLAGMWQLISGLERVSKTLVWDREAAIGGTGKLTAPAAAFAGTLAT"
BASE COUNT          249 a          592 c          641 g          273 t
ORIGIN      
        1 ccgatgctcg acctgaacgg tcccgctgac ccgatcgagg actacctcgc cgcattgcaa
       61 ctgctcgagg gcgtggcgca cgacgtcgat gtcgtggtcc ccggccacgg gtcgatcggc
      121 ggacctgatc gggtgcagtc acggatcgac caggatcggg cgtacgtgca cgccgtgcgt
      181 gaccgccaga ttccgagcga cccgcgggtc ggcccatcgg gctcggatgg cgaggatccg
      241 ttgcctgacg tacacgaccg gcaggtacag cagctcgccc gaagaagcgc gagagcgtct
      301 gtccaggtcg cggatgctgc ggttgctgct cagggcgcga gcggcagcgc gcagcgccag
      361 cagcagctca tcgttggacg tcatcaggtg ttcccagttc aaccggtcgg gcgggtacgg
      421 cacgcggcac gtcggacggt gctgcggcgg cagaggcgaa gcgacgtgcg ccgggttctg
      481 cccccaccca gctcggtggt cggggacgca catgagggcg gcgctctggt gcgcgtccct
      541 gactctaggg gttccgaacg ccgtccccgc cgatttcgcg ggctcgacgt tgtccgggcc
      601 gctgcgacgt gacagagttc tcccgcttgg atggttgacg aacacagctg cagctcgatg
      661 tcccgtccca gcgcccccac ccgggtggct gcgtctcccc tgtcgatccg agcccggacc
      721 gtggtgggga agcgcacgca ctcgtgacgg cgcgtcgcgg aaggcggccg tccttgtccc
      781 gttcctgccc ggtcccagcc tccaccccgc cgtcgcgcgc gctccgcgcc acgagctgta
      841 cggcggcgcg agagcgactc gacgacacct ctctcgcctc cccggctgaa ggagcacccc
      901 agtgaccgaa cgggcccatg aggtgcgcgg acctctggcg ctcggggtcg ccgtgggggt
      961 ggcgctgcgt gcgctcagcg atctcacacc ggccgctgcc ggggtgagag acgggcgact
     1021 caaggtgctg ctgcgcgcca gcctcgccct gtcgacgact cgtgaactct gacccccgta
     1081 ggtaccggga aagttgaccc cttcccgtga ggctgcggga ggtgatgact gtggaggact
     1141 gggcagaggt ccgtcggctg caccgtgccg agggcttgcc gatcaaggag atcgtccgtc
     1201 agacggggtt ggcgcggaac acggtgcgga cggcgctgcg tagcgagcgg gcaccgacgt
     1261 accagcggcc gtcgcggggt tcggtggtcg acgcctatga gccagcgatc cgggtgctgc
     1321 tcgcctcatg gccgcggatg ccgggcccgg tgatcgcgca acgcatcggc tggtcgtatt
     1381 cggaggggcc gctcaagaag cgcctggcgc tgatccgccc ggagtacctc ggtgtcgatc
     1441 cggtcgaccg ggtcgcctac cagcccgggg agatcaccca gtgcgacctg tggttccccg
     1501 acacgaagat cccggtcggg aacgggcaac gctggatgct gccggtgctg gtcatggtgc
     1561 tgggctactc gcgctacctg tcggcgacga tgctgccgtc gcggcaggcc ggggacctgc
     1621 tggcggggat gtggcagctg atctccgggc tcgagcgggt ctcgaagacc ctggtctggg
     1681 accgcgaagc agcgatcggc ggcaccggga agctcaccgc cccagccgcg gcgttcgccg
     1741 ggacgctggc gacca
//
LOCUS       DASRSC010000350        10249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3095562,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000350 DASRSC010000000
VERSION     DASRSC010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10249
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3095562"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VFE39_16925"
     CDS             <1..396
                     /locus_tag="VFE39_16925"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET6260322.1"
                     /translation="LSAPPVEEIKVGTQILQSLNLRPRKLEIVSCPSCGRAQVDVYKL
                     ADEVTAGLTGMEVPLRVAVMGCVVNGPGEAREADLGVASGNGKGQIFVRGQVIKTVPE
                     HAIVETLIEEAMKIAETMEAEGEPQVTVG"
     gene            543..1376
                     /locus_tag="VFE39_16930"
     CDS             543..1376
                     /locus_tag="VFE39_16930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET6260323.1"
                     /translation="MLRVAGARLLDDRDRAGVCAVLESDPVAACMVAARVEVAGLDPW
                     RLGGELWGVGSRFDGLCFSGANLVPLRGERSALRSFADRARRYGRTCSSLVGRAELVL
                     PLWNELAGHWAPAREVRADQPLMALAGRPGVAADPLVRPVRPEELDRYLPAAVAMFTE
                     EVGIDPRAGDGGVGYRSRVAELVAAGRAFARFEGDEVVFKAEIGALSSQVGQIQGVWV
                     HPALRGRGLGTAGTAAVVERLAALGRMSSLYVNGFNLVARSAYHRIGFTQVGSFATIL
                     F"
     gene            1391..2200
                     /locus_tag="VFE39_16935"
     CDS             1391..2200
                     /locus_tag="VFE39_16935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HET6260324.1"
                     /translation="MTQEPSGFRPSPSIDTEVAHSPRIWNYWLGGKDNYEVDRRIGDD
                     FRELFPPIVDIARSSRAFLKRAVQHLAGEAGVRQFLDIGTGLPTVDNTHEVAQRVAPE
                     SRIVYVDNDPIVLAHARVLLLSSAEGATTYIDADLYDSAAILDVAARSLDFTRPIALM
                     LMNILGHVPDFDQAREIVTRLVAALPPASYLVVADGTTDDGPFDAAIQMWNDAGSLPY
                     ILRTPEQIGQYFDGLELLEPGVVSCPFWRPDESHVGAPSHVDEFGAVGRTR"
     gene            2254..2781
                     /locus_tag="VFE39_16940"
     CDS             2254..2781
                     /locus_tag="VFE39_16940"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET6260325.1"
                     /translation="MAAASVVEGDWRARLEEFGRQKWRLYLRHPWMLQIAQGRPMLGP
                     NSMASTDIVLRAMDELGLDEHEMLAAVVLLSSFVAGLAKTTIEAMQAADRTGVSDDEW
                     WEIQGEYVGPAVAEGQLPMMTKVGEAGAFTSMFDHFEFGLQRVLDGLGALIDARSGAA
                     RPPADQAKRTTGCGQ"
     gene            complement(2869..4152)
                     /locus_tag="VFE39_16945"
     CDS             complement(2869..4152)
                     /locus_tag="VFE39_16945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6260326.1"
                     /translation="MIILDTALRRREAEGRPIRVGMIGAGFMGRGLANQIVNSVPGMN
                     LVAVANRTLANAERAYGEAGVEPVRVENASELDTAIEKGTPAVLEDAFELLKAVHLDC
                     IVDVTGAVEFGARVTVAAIERGLPVVTMNAELDGTVGPLLALRARDAGVVLTGCDGDQ
                     PGVQGNLMRFVQGLGVTPLVAGNIKGLQDEYRTPTTQKAFAERWGQDPHMVTSFADGT
                     KISFEQAIVANAFGLTVPRRGMNGWEHEGHVDELTDRYDVDRLRELGGIVDYVVKSKP
                     GPGIFVLGTHDDPKQRHYLELYKLGKGPLYSFYTPYHLCHFEVPNSVVRAVDFGDAAL
                     TPPAGPHVDVVATAKQDLRAGRAVDGLGGYDTYGVAETTSITRSEGLLPMGVAEGCVL
                     KRDVQKDAVLTYADVTLPPGRLVDQLREEQAKLFA"
     gene            complement(4149..4775)
                     /gene="rfbC"
                     /locus_tag="VFE39_16950"
     CDS             complement(4149..4775)
                     /gene="rfbC"
                     /locus_tag="VFE39_16950"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HET6260327.1"
                     /translation="MIDSPGRPEEDDPSPMQIHTTPLPGAALVDLKLLEDDRGFFARA
                     FCRQEFVDAGLEPLVEQANISYNHKAGTLRGFHYQLEPNAETKLIRCYRGAIYDVIVD
                     LRQESPTYLQWFGAELSEDNRTAMYVPRLFAHAYLTLTDAAEVIYQVSTPYTPGAERG
                     LRWDDPALAVDWPIPPAIVSEKDAAWPLLSAAGSDGAPRIGTASATAR"
     gene            4949..5365
                     /locus_tag="VFE39_16955"
     CDS             4949..5365
                     /locus_tag="VFE39_16955"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6260328.1"
                     /translation="MTCSSTTCAQPPGPGRSAHGADVLKVGHGPGLLLGLLARRDDVG
                     RIVGVDPSVEMRRLAVRRLAAEIATGRLELRRGDAAATGLPDASVDIVVSVNSVAIWP
                     ELERVENGLRSRFADVRRMRTRRCTVFDARVENTLP"
     gene            5473..7254
                     /locus_tag="VFE39_16960"
     CDS             5473..7254
                     /locus_tag="VFE39_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HET6260329.1"
                     /translation="MLAALAVLLVTAGCGLFGDDGPKNVATAFLTAWSAGDDARAAAL
                     TDDPVAAGQLLGVTREALGPATLVAELAQVRTATARAAASVEMTWQLGPDRTWTYLNE
                     LELLPVPDTEEGWVVHWGPTVVHPDLAARQRLELRTDPAQPAPVVDRAGVPLLTATPV
                     VAVLLDRTTAGDLPAVTGALARGLSPLDPQITQESITEGAARTPDGQVYPVALLRDTD
                     YQGVKQAIHDLPGVRFSSAERLLAPDAGFARQVLPAVRTETAAQLEGVAGWSVLLVDS
                     SGATIRTLAEEPPRPGSTVTIGMDRAVQTAAEDAVEPVAQQAMLVAVAPSSGDLLAVA
                     QNGPADVAGALALTGRYPPGSTFKIVTATAAAAEDGLSATSPVACPGSTVIGGRPVPN
                     SGLFDLGTVPLLTAFARSCNTTFARIASQLDAAALPAAALRLGLGADYAVPGLTTITG
                     SVPVSADAVQRAEDGFGQGEVLATPLGMALTAATVAHGAPVVPQLLRDRPTQVLRPAA
                     QPIAAELEQLRPMMRAVVTDGTATQLAGLGEVFGKTGTAEFTNGGRSHGWFVGYRGDV
                     AFAVLVVDAGSSTPAIEVAARFLSTIG"
     gene            7309..7689
                     /locus_tag="VFE39_16965"
     CDS             7309..7689
                     /locus_tag="VFE39_16965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HET6260330.1"
                     /translation="MSLHRIAALALAADVIAVVVFAAIGRISHAESGSLLGLLGTAAP
                     FLVGLAAAWATPVVRAQPPGLRAGAVVLAGTAVLGLAVRAAFTGRLPLSFAVVTVVTL
                     AVLLLGWRALSLLVSHRAASRALR"
     gene            7803..8660
                     /gene="map"
                     /locus_tag="VFE39_16970"
     CDS             7803..8660
                     /gene="map"
                     /locus_tag="VFE39_16970"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HET6260331.1"
                     /translation="MPARTLLTPGTVSPTRPVPAQIPRPEYVGKDRPSRSNDPWVQTP
                     EVIEAMRVASRIAAQALQVGGEAVAPGVTTDEVDRVVHEFLVDHDAYPSTLGYKSFPK
                     SCCTSLNEVICHGIPDSTVIEDGDIVNIDVTAYIGGVHGDTNATFLAGDVAEEDRLLV
                     ERTREATMRAIKAVRPGRELNVVGRVIESYAKRFGYGVVRDFTGHGIGRSFHSGLVVL
                     HYDEPDVHTVLEPGMTFTIEPMITLGTIDYDIWPDAWTVVTKDRARTAQFEHTVLVTA
                     DGVEILTLP"
     gene            complement(8647..9093)
                     /locus_tag="VFE39_16975"
     CDS             complement(8647..9093)
                     /locus_tag="VFE39_16975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260332.1"
                     /translation="MTPPRPDVAALLRAGRLQRVPADSEAAVARLVVAEQHLDTARRL
                     LDEQLDLEIAYVALYDAARKAVTAAMLAGGLRATNRTGAHEAVAIWCAESLGQRNPAA
                     RHFDALRRRRNRSEYDDLVLSGADVSVDLADAAAIVAAATAAVRAA"
     gene            complement(9090..9701)
                     /locus_tag="VFE39_16980"
     CDS             complement(9090..9701)
                     /locus_tag="VFE39_16980"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6260333.1"
                     /translation="MSQSAPPLLPIFRSAAQLAVLAELFTGSARELSIGELARRTGVP
                     QATVSREVARLVGAGILADRDVGRTRLVSARAESPIHAELTALLLKVAGPPAVLRELL
                     TDVAGVREAYIYGSWARRQHGEPGDEPADIDVLVVADDGPDDDRAATVRRVRAVADEA
                     TDRLGRDVTVSVLTPAEWTEGRGGFVRSVRDSPLVPLDLRPAG"
     gene            complement(9733..>10249)
                     /locus_tag="VFE39_16985"
     CDS             complement(9733..>10249)
                     /locus_tag="VFE39_16985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016335038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6260334.1"
                     /translation="YTEGLTPFWDALHEYWRDPAAGRDAVQASLHLDSTRWQYVHGVP
                     AGRLDRVSPDTWTLDQALLDRPGNAEIQMQLFWDYRTNLDAYPAFHAYFRRHRPPTLI
                     VWGEHDEIFGVDGARAYLRDLPDAELHLLDAGHFALETHGPEIAALIRDFLPRALRGT
                     GDSDHRAAASA"
BASE COUNT         1347 a         3609 c         3748 g         1545 t
ORIGIN      
        1 ctctcggcgc cacccgtcga ggagatcaag gtcggcacgc agatcctgca gtcgttgaac
       61 ctgcgtccgc gcaagctgga gatcgtgtcc tgcccgtcct gcggacgcgc gcaggtggac
      121 gtctacaagc tcgccgacga ggtcacggcc gggctcaccg gcatggaggt gccgctgcgc
      181 gtggccgtca tgggttgcgt cgtcaacggc ccgggggagg cccgtgaggc cgacctgggc
      241 gtggcgtcgg gcaacgggaa gggccagatc ttcgtcaggg gccaggtcat caagaccgtt
      301 ccggagcacg ccatcgtcga gaccttgatc gaggaggcga tgaagatcgc cgagacgatg
      361 gaggccgagg gggagccgca ggtgaccgtc ggctgagcgg agcgggccgc gcatcggcac
      421 cgtcggctga gcggagcggg ccgcgcatcg gcaccgtcgg ccgagcggag cgtggcgagt
      481 tcgatcctct ggtgagcgat cccgtcgtgt gcatcgcggc gatcgtctgg catcgttaag
      541 gagtgcttcg tgtcgccgga gcgcggctcc tcgacgaccg ggaccgcgcg ggagtatgcg
      601 cggtcctgga gagtgatccg gtggccgcct gcatggtcgc cgctcgtgtc gaggtggcgg
      661 ggctcgatcc ctggcgtctt gggggcgagc tgtggggtgt gggttcccgg ttcgacggtc
      721 tctgtttctc cggcgccaac ctcgtaccgc tgcggggcga gcgctcggct ctgcgcagct
      781 tcgccgatcg ggcccgccgg tacgggcgca cctgctcgtc cctcgtcggc cgggcggagc
      841 tggtgctgcc gctgtggaac gagcttgcgg gccactgggc gcccgcccgg gaggtgcgtg
      901 ccgaccagcc gctgatggcg ctcgccgggc gacccggggt ggccgccgac ccgctcgtgc
      961 gaccggtacg tcccgaggag ctggaccgct acctgccggc ggccgtcgcc atgttcacgg
     1021 aggaggtcgg catcgacccc cgtgccggtg acggcggggt cggttaccgg tcgcgtgtgg
     1081 ccgagctcgt tgccgccggg cgggcgtttg cccgtttcga gggcgacgag gtcgtgttca
     1141 aggcggagat cggggcgctg tcgtcccagg tcgggcagat tcagggcgtg tgggtgcacc
     1201 ccgccctccg gggccgcgga ctcggcacgg ccggtaccgc cgcagtggtc gagcgcctgg
     1261 ctgcgctggg ccggatgtcg agcctgtacg tcaacggctt caacctggtg gcgaggtccg
     1321 cgtatcaccg gatcggattc acccaggtcg gcagcttcgc cacgatcctg ttctagaagg
     1381 gaccgcgcgc gtgacgcagg aaccgtccgg cttcaggccg tcgccatcga tcgacacaga
     1441 ggtggcgcac tccccgcgga tctggaacta ctggctcggc ggcaaggaca actacgaggt
     1501 cgaccggcgc atcggcgacg acttccgtga gctcttcccg ccgatagtcg atatcgcccg
     1561 gtcctcgcgg gccttcctca agcgggccgt gcagcacctg gccggggagg ccggggtccg
     1621 ccagttcctc gacatcggca cggggttgcc gacggtggac aacacccacg aggtcgcgca
     1681 gcgggtggcg cccgagtcgc gcatcgtcta cgtcgacaac gacccgatcg tcctggctca
     1741 cgcgcgggtg ttgctgctca gctcggccga gggagccacc acttacatcg acgccgacct
     1801 ctacgactcg gccgcgattc tcgacgtcgc cgcccggagt ctcgacttca cgcggccgat
     1861 cgcgctcatg ctcatgaaca tcctcggcca cgtccccgac ttcgaccagg cccgggagat
     1921 cgtcacacgg ctggttgctg ccctgccgcc tgccagctat ctggtggtgg cggacgggac
     1981 caccgacgac ggcccgttcg acgccgccat ccagatgtgg aacgacgccg gatcgttgcc
     2041 gtacatcctc cgcaccccgg agcagatcgg gcagtacttc gacggactgg agctgctcga
     2101 gccgggcgtc gtctcctgcc cgttctggcg gcccgacgag agccacgtcg gcgcgcccag
     2161 ccacgtggac gagttcggcg ccgtcggccg cacgaggtga gcgccgccgg cgctcagccc
     2221 gccgtggcgt ggtagcgggt gtacggcgag gacgtggcag ccgcgagcgt cgtcgagggc
     2281 gactggcggg cgcgcctgga ggagttcggg cgccagaagt ggcggctgta cctgcgacac
     2341 ccgtggatgc tgcagatcgc gcaggggcgc ccgatgctcg gcccgaactc gatggcgtcc
     2401 actgacatcg tgctgcgggc catggacgag ctcggcctcg acgagcacga gatgctcgca
     2461 gcggtggtgc tgctcagctc gttcgtcgcc gggctggcca aaaccacgat cgaggcgatg
     2521 caggcggccg accgcacggg cgtcagcgac gacgagtggt gggagatcca gggcgagtac
     2581 gtcggccccg cggtcgccga gggacagctg cccatgatga ccaaggtggg cgaggcgggg
     2641 gccttcacct cgatgttcga ccatttcgag ttcggcctcc agcgggtgct cgacggcctc
     2701 ggtgcgctga tcgacgcgcg cagcggtgcc gctcgaccgc ccgctgacca ggcgaagcgg
     2761 accacagggt gcggccagta ggaatccccg atggaagcgg ttcgcagctc atgcacccgg
     2821 gcgggcgagc tgcgacccgc tcgcccgctc tcggtcgttc gcggtccctc aggcgaagag
     2881 cttcgcctgc tcctcgcgca gctggtccac gagccgcccc ggcggcaggg tgacgtcggc
     2941 gtaggtgagc acggcgtcct tctggacgtc ccgcttgagc acgcagccct cggccacccc
     3001 catcggcagc aggccctcgg accgagtgat gctggtggtc tcggctacgc cgtaggtgtc
     3061 gtacccgccg aggccgtcga cggcccggcc ggcccggagg tcctgcttgg ccgtcgctac
     3121 cacatcgacg tgcgggcccg cgggcggggt gagggctgcg tcgccgaagt ccacggcgcg
     3181 gaccacggag ttgggcacct cgaaatggca taggtggtag ggggtgtaga agctgtacaa
     3241 ggggcccttg cccagcttgt agagctccag gtagtgccgc tgcttcgggt cgtcgtgcgt
     3301 gccgagcacg aagatgccgg gacccggctt cgacttcacc acgtagtcga cgatcccgcc
     3361 cagctcgcgg agccggtcca cgtcgtagcg gtccgtgagc tcgtcgacgt gcccctcgtg
     3421 ctcccagccg ttcatgccgc gccttggcac ggtgagcccg aacgcattcg ccacgatcgc
     3481 ctgctcgaag gagatcttcg tgccgtcggc gaagctcgtg accatgtgcg ggtcctggcc
     3541 ccagcgctcg gcgaacgcct tctgcgtggt cggcgtgcgg tactcgtcct gcagcccctt
     3601 gatgttgccc gcgaccagtg gcgtcacccc aaggccctgc acgaaccgca tcaggttgcc
     3661 ctgcacgcct ggctggtcgc cgtcgcagcc ggtgagcacg acaccggcgt cgcgggcccg
     3721 gagggcgagc agcgggccga ccgtgccgtc cagctccgcg ttcatggtga cgacgggcag
     3781 gccccgctcg atcgccgcca cggtgacgcg cgcgccgaac tcgaccgcgc cggtgacgtc
     3841 gacgatgcag tccaggtgga cggccttgag cagctcgaag gcgtcctcga gcaccgcggg
     3901 ggtgcccttc tcgatcgcgg tgtcgagctc ggaggcgttc tccacccgca cgggctcgac
     3961 gcctgcctcg ccgtaggcgc gctcggcgtt ggccagggtc cggttggcca cggccacgag
     4021 gttcatgccc ggcaccgagt tgacgatctg gttggccagg ccccggccca tgaacccggc
     4081 gccgatcatg ccgacgcgga tcgggcggcc ctcggcctcg cgcctgcgca gcgcggtgtc
     4141 cagaatgatc atcgggccgt cgctgacgct gtgccgatgc gcggggcgcc gtcgctgccc
     4201 gccgcggaga gcagcggcca tgccgcgtcc ttctcggaca cgatcgccgg cgggatcggc
     4261 cagtcgacgg cgagcgccgg gtcgtcccat cgcagaccgc gctccgcgcc gggggtgtac
     4321 ggcgtgctga cctggtagat gacctcggcg gcatcggtca gggtcaggta ggcgtgcgcg
     4381 aacaggcgcg gcacgtacat cgccgtgcgg ttgtcctcgg agagctcggc gccgaaccac
     4441 tgcaggtagg tgggcgactc ctgccgcagg tcgacgatca cgtcgtagat cgcgccccga
     4501 tagcagcgga tgagcttcgt ctccgcgttg ggctcgagct ggtaatggaa cccgcgcagc
     4561 gtgccggcct tgtggttgta ggagatgttg gcctgctcga ccagcggctc gaggcccgcg
     4621 tcgacgaact cctgccggca gaatgcgcgc gcgaagaaac cgcgatcgtc ctcgaggagc
     4681 ttcaggtcga cgagtgccgc gccggggagc ggggtggtgt ggatctgcat gggggaggga
     4741 tcgtcctctt cgggtcgtcc ggggctgtcg atcacgtcgt gtcgcacgat ggtagatggg
     4801 cgggtggcga tcggctcacc gccgatcagc gcgtgcagcg caccccggag gtacctgcag
     4861 tcgcgtaccg gctccgcccg ggcagtcccg tccggccacc agcacctcgc gggccctctg
     4921 cgcccgcgcg atctgggcgt cgatcgcggt gacctgctcg tccaccacct gcgcccaacc
     4981 gcccggtccc ggtcgcagcg cccacggtgc cgacgtcctg aaggtcggtc acgggcccgg
     5041 cctcctcctg gggctgctgg cccgccggga cgacgtcggg cgcatcgtcg gggtggatcc
     5101 ctcggtggag atgcggcggc ttgcggtgcg caggctcgcg gccgagatcg ccaccggacg
     5161 actggagctc cggcgcggtg acgccgccgc gaccggcctg ccggatgcgt cggtggacat
     5221 cgtcgtctcg gtcaactcgg tggcgatctg gcccgagctc gagcgcgtcg agaacgggct
     5281 gcgatcacgc ttcgccgacg tccgccggat gcggacccgg cgctgcacgg tgttcgacgc
     5341 gcgggtggag aacacgcttc cgtaacctct gatcgattgt ctcgcggtag ccgaatgggc
     5401 cggcggccgg ctcggcatac tgaccgggtg ctccctaccc gtccgattcg tgcagctcgg
     5461 cggatgcgcg tgatgctcgc tgcgctggcg gtgctgctcg tcacggccgg gtgtgggctc
     5521 ttcggcgacg acggcccgaa gaatgtggcc accgcgttcc tcacggcatg gtcggcaggc
     5581 gatgacgcgc gtgcggcggc cctcaccgac gatccggtgg ccgctggtca gctgctgggc
     5641 gtcacccgcg aggcgctcgg tccggcgacc ctggtcgccg agctcgccca ggtgcgcacg
     5701 gccaccgcgc gggccgccgc gtccgtcgag atgacctggc agctggggcc ggaccgtaca
     5761 tggacctacc tcaacgagct cgagctgctg cctgtacccg acaccgaaga gggctgggtc
     5821 gtgcactggg ggcccactgt cgtgcatccc gacctggccg cccgacaacg gctggagctg
     5881 cgcaccgacc cggctcagcc ggcccccgtc gtcgaccgcg ccggtgttcc cctgctcacg
     5941 gccaccccgg tcgtcgccgt gctgctggac cggacgaccg cgggcgacct gccggccgtg
     6001 acgggggcgc tcgcccgcgg gctctccccg ctggatccgc aaatcacgca ggagtcgatc
     6061 accgaagggg cggcgcgcac accggacggg caggtctacc ccgtcgcact actgcgcgac
     6121 accgactacc agggggtgaa gcaggcgatc cacgatctgc ccggcgtccg cttcagcagc
     6181 gccgagcggc tgctggcgcc ggacgccggt ttcgcccggc aggtgctgcc cgcggtccgc
     6241 accgagaccg ccgcgcagct cgagggtgtg gcgggctggt ccgtgttgct cgtcgattcc
     6301 agcggcgcca ccatccgcac gctggccgag gagccgccgc gcccgggcag caccgtcacg
     6361 atcggcatgg accgcgccgt gcagaccgcg gctgaggatg ccgtcgagcc ggtggcgcag
     6421 caggcgatgc tcgtcgcggt ggcgccgtcg tccggtgatc tgctggccgt cgcgcagaac
     6481 ggtccggccg acgtcgcggg tgcgctcgcc ctgaccgggc gctacccacc gggttccacg
     6541 ttcaagatcg tcacggctac ggcggcagcc gccgaggacg ggctctcggc gacatcgccg
     6601 gtggcctgcc ccggcagcac cgtcatcggc ggccgcccgg tgcccaactc cggcctcttc
     6661 gacctcggca ccgtcccgct gctcacggcg ttcgcccggt cctgcaacac cacgttcgcg
     6721 cgcatcgcgt cacagctgga tgcggccgcg ctgccggcgg cggcgttgcg actcggtctg
     6781 ggggccgact acgcggtgcc ggggctcacc accatcaccg gctccgtgcc tgtctcggct
     6841 gacgccgtgc agcgcgccga ggacgggttc ggccaggggg aggtgctggc caccccgctg
     6901 gggatggctc tcaccgcagc cacggtggcc cacggcgcgc cggtggtgcc gcagctgctg
     6961 cgcgaccggc ccacccaggt gctgcgtccg gccgcccagc cgatcgccgc cgagctggaa
     7021 cagctgcgcc ccatgatgcg ggcggttgtc accgacggca cggccacgca gctcgccgga
     7081 ctcggtgagg tgttcgggaa gaccggcacc gccgagttca ccaacggcgg gaggtcccat
     7141 ggctggttcg tcggctaccg gggagacgtg gcgttcgcgg tactggtggt cgacgccggg
     7201 tcgtccactc ccgccatcga ggtcgcggcg cggttcctgt ccacgatcgg ctgatcgacg
     7261 cgggtcgcgc agatccacgc ccttgctcgg gcccttaggc tggagcgcat gtccctccac
     7321 cggatcgccg cgctcgcgct cgcggctgac gtgatcgcgg tggtcgtctt cgccgccatc
     7381 ggccggatca gccacgccga gtccgggagc ctgctgggcc tgctcggcac cgcggcgccc
     7441 ttcctggtcg ggctcgccgc cgcctgggcc acgccggtcg tgcgggcgca gccgcccggg
     7501 ttgcgggcag gtgcggtggt gctcgccggc acggccgtgc tcgggctcgc cgtgcgggcg
     7561 gcgttcaccg gacgtctccc actgagcttc gccgtggtca cggtcgtcac gctcgccgtg
     7621 ctgctgctcg gctggcgggc gctgtcgttg ctggtctctc accgcgccgc gagccgcgcc
     7681 ctccgctgat cacaccggtt ctgccgctcg agcgccggcc ctccgtcgag gtatccgccg
     7741 gcaccacacc ggtgtgagcc tgaccccgct gtccgtgccg gcgcggtgct tacactggcg
     7801 gcatgcccgc ccgcacgctg ctgacccctg gcaccgtctc gccgacccgt cccgttcccg
     7861 cgcagatccc acggccggag tacgtcggca aggaccggcc gtcccgcagc aatgacccgt
     7921 gggtgcagac gcccgaggtg atcgaggcca tgcgggtggc cagccggatc gcggcacagg
     7981 cgctgcaggt gggtggcgag gccgtggcgc ccggtgtcac caccgacgag gtcgaccgcg
     8041 tggtccacga gtttctcgtc gaccacgacg cctacccgtc cacgctgggc tacaagagct
     8101 tcccgaagtc gtgctgcacg agcctcaacg aggtgatctg ccacggcatc cccgactcga
     8161 ccgtgatcga ggacggcgac atcgtgaaca tcgacgtcac ggcgtacatc ggcggcgtcc
     8221 acggcgacac gaacgccacg ttcctcgccg gtgacgtcgc cgaggaggac cggctcctgg
     8281 tcgagcgcac ccgcgaggcc acgatgcggg cgatcaaggc ggtgcgcccg gggcgtgagc
     8341 tcaacgtcgt gggtcgcgtg atcgagtcct acgccaagcg cttcggctac ggcgtggtgc
     8401 gcgacttcac gggccacggc atcgggcggt ccttccacag cgggcttgtc gtgctgcact
     8461 acgacgagcc ggacgtgcac acggtcctcg agccgggcat gacgttcacg atcgagccga
     8521 tgatcacgct cggcacgatc gactacgaca tctggcccga cgcgtggacc gtcgtcacca
     8581 aggaccgcgc caggactgcg cagttcgagc acaccgtgct ggtcaccgcc gacggtgtgg
     8641 agatcctcac gctgccctga cggctgccgt cgccgccgcg acgatcgcgg ccgcgtcggc
     8701 gaggtcgacc gagacgtcgg cgccgctcag gacgaggtcg tcgtactccg agcggttgcg
     8761 tcggcggcgc agggcgtcga agtgccgagc ggccgggttg cgctgcccga ggctctccgc
     8821 gcaccagatc gccaccgcct cgtgtgcgcc ggtccggttc gtcgcccgca gcccgcctgc
     8881 gagcatcgcc gcggtgacgg ccttgcgcgc cgcgtcgtag agcgcgacgt aggcgatctc
     8941 caggtccagc tgctcgtcga gcaggcggcg cgccgtgtcg agatgctgct ccgcgacgac
     9001 gaggcgggcc acggcggcct cggagtcggc gggcacccgc tgcaggcggc cggcgcgcag
     9061 cagcgcggcg acgtccggcc ggggcggggt caccctgcgg gccgcaggtc gagcggcacc
     9121 agtggggagt cgcgtacgga gcgcacgaac ccgccgcgtc cctcggtcca ctccgccggc
     9181 gtcagcacgc tcaccgtgac gtcccggccg aggcggtcgg tggcctcgtc cgccacggcg
     9241 cgaacgcgtc gcaccgtggc ggcccggtcg tcgtcgggcc cgtcgtcggc caccaccagg
     9301 acgtcgatgt ctgcaggctc gtcgccgggt tcgccgtgct gccgacgagc ccagctgcca
     9361 tagatgtagg cctcacgcac ccccgccaca tcggtcagca gctcgcgcag caccgcgggc
     9421 ggccccgcaa ccttgagcag cagggccgtg agctcggcgt ggatcgggga ctcggccctg
     9481 gcgctcacca gccgggtgcg cccgacgtcg cgatcggcga gaatgccggc gccgaccagg
     9541 cgcgcgacct cccgggacac cgtcgcctgc ggaacgccgg tgcgtcgcgc cagctcaccg
     9601 atcgagagct cccgtgcgct gccggtgaac agctcggcga gcacagccag ttgcgccgcc
     9661 gagcggaaga tcggcagcag cgggggcgca gactgactca caacctggat gatttcatcc
     9721 tgagtgtgga tgtcaagcgg acgccgccgc gcgatgatct gagtcgcccg tcccgcggag
     9781 ggcgcggggc aggaagtcgc ggatcagtgc ggcgatctcg gggccgtggg tctccagggc
     9841 gaagtggccg gcgtcgagca ggtgcagctc ggcgtcgggg agatcgcgca ggtaggcgcg
     9901 tgcgccgtcg acgccgaaga tctcgtcgtg ctcgccccag acgatgagcg tgggcgggcg
     9961 gtggcggcgg aagtaggcgt ggaacgcggg gtaggcgtcg aggttggtgc ggtagtccca
    10021 gaacagctgc atctggatct cagcgttgcc cggtctgtcg agaagggcct ggtcgagcgt
    10081 ccaggtgtcg gggctgacgc ggtcgagccg cccggccggc accccgtgga cgtactgcca
    10141 gcgcgtgctg tcgaggtgca acgacgcctg cacggcgtcc cggccggcgg ccgggtcacg
    10201 ccagtactcg tgcagggcgt cccagaacgg cgtcaagccc tcggtgtag
//
LOCUS       DASRSC010000351         5756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3587368,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000351 DASRSC010000000
VERSION     DASRSC010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5756
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3587368"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1448)
                     /locus_tag="VFE39_16990"
     CDS             complement(99..1448)
                     /locus_tag="VFE39_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET6260335.1"
                     /translation="MTQPPDRVPARTVLRLAAPALPVLAAEPLYLLVDTAVVGRLGAV
                     PLAGLAVAGVVFAQVTSQLNFLSYGTTARAARLHGAGRRGAAVAEGVQATWLALAVGL
                     LVLVVGQLVARPVAAALGNGGAIADAAVSWLRIALCGAPLVLVTLAGNGWMRGVQDTA
                     RPLRFVLAGNGLSALACILLVHGVGGWAGLGLVGSAVANVVGQTVGASLFLGALVRAA
                     RADGVALRPAPSVLRSQLGLGRDLMARSLAFQACFLSAAAVATRFGAATAAAHQIVLQ
                     LWFFQALVLDAVAIAAQSLVGAALGSPERGAERARALAGQVTRYGIVLGLGFGALFAA
                     LSGVLPPVFTPDPEVLAAVPVPWWFFVAMQPVAGVVFALDGVLLGAGDAAYLRTSTLA
                     AAVVGFLPLIWASLAWGWGLTGIWAGLMAFMLIRLVAVGRRARSDRWAVTGAVRPGA"
     gene            complement(1472..2119)
                     /locus_tag="VFE39_16995"
     CDS             complement(1472..2119)
                     /locus_tag="VFE39_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020671024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET6260336.1"
                     /translation="MTSDTGPTRIEPDARAARRAFLVAALRRPATMGAVAPSSPRLGA
                     VLASVVPRCGSPVVVELGPGTGAVSAVIATKLPAGARHLAVELDSDMVAFLRRTRPEL
                     EVVPGNAADLGALLALRGVTSVDAVICGLPWALFDDATQTALLGEISRAIGATGAFTT
                     FAYLHGMTLGAARRFRRTLRETFDEVLVTATVWRNLPPAFVYVCRRPHAVPRADG"
     gene            complement(2231..2482)
                     /locus_tag="VFE39_17000"
     CDS             complement(2231..2482)
                     /locus_tag="VFE39_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260337.1"
                     /translation="MNASHSEGPIPVTVTVDDAHLAAIDELAARLRAAGMDVEQVLAP
                     VGVITGSAPSGWEALADLDGVAGVEPQRTMRLPPPGTPQ"
     gene            complement(2479..3693)
                     /locus_tag="VFE39_17005"
     CDS             complement(2479..3693)
                     /locus_tag="VFE39_17005"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET6260338.1"
                     /translation="MMRPSREPAASTTGRYLLVINDDLFGDDEASRAVVREVSGVQEV
                     VSTRDAQPGALDARQTGPGALLFAELGVAVVTVVPDRVGATGLFEPADDRLIAVEPER
                     IVHALQDAVPVDALQDSPFIDDAEATWGLQATGVLAAAESGAGISVAVLDTGIDLRHP
                     DFADRDITSRSFVDGQTAQDVQGHGTHCAGTMAGPVDPAEGPRYGVANGARLLVGKVL
                     SDQGSGTDADILAGMSWAISAGAQVISMSLGADQREISTAYETVGRRALAAGTLVVAA
                     AGNNASRSEGDPGFVGVPANSPSIMAVGAVDATLAVADFSASATEVGGGEIDIAAPGV
                     QVHSSWPLPRRYNTISGTSMATPHVAGIAALWAERSGARGRALWDELVGAAQPLGPPA
                     QDVGAGLVQAPS"
     gene            complement(3762..4760)
                     /locus_tag="VFE39_17010"
     CDS             complement(3762..4760)
                     /locus_tag="VFE39_17010"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET6260339.1"
                     /translation="MGGSLDAAVAAAAGLLMRARDVTLLAHVNPDADSLGSALALGIV
                     LRRHGAAVRVSFAAPEQVPETLVPLDVLGLVVGPEQVPVAPELLVVCDAAEPSRLGAL
                     ADRLATAGSSVMIDHHVSNPGFGDVQVLDPGVEATVVLAHRVLVAMGEPLDADVARCL
                     YAGLVTDTRGFRTAGEAAHRMAADLVATGVDPEELVRPIMDTHPYVWLATLGEVLQNA
                     VLEPDAAGGLGLVHACVPFEDTTRFRAEEVDSIVDVLRTTAEAEVAAVVKQVGHRRWV
                     TSLRSKGRVDVAAAAGLLDGGGHRAAAGFTRDGTADEVLAALRGALAALDPAKRPA"
     gene            complement(4790..5239)
                     /gene="rbfA"
                     /locus_tag="VFE39_17015"
     CDS             complement(4790..5239)
                     /gene="rbfA"
                     /locus_tag="VFE39_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET6260340.1"
                     /translation="MVDQARARRLAKRISQIVAAALEHEVKDPRLKMVTITDTRVTGD
                     LREATLYYTVIGETVDADPDTAGAAAALASATGVLRSLVGAGTGIRHTPSLTFVPDQV
                     PDEARRMEELLARTRESDAEVARLAAGARPAGDPDPYRTPRERADEE"
     gene            complement(5258..5557)
                     /locus_tag="VFE39_17020"
     CDS             complement(5258..5557)
                     /locus_tag="VFE39_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HET6260341.1"
                     /translation="MFVGALELDVLLGDIHSLKQKRSVVRPVLAELRRKFEVAVAEAG
                     HLDLHRRALLGVSCVAADHGHVTEVLDRCERLVAARPELELLSARQRMLGPDDDS"
BASE COUNT          866 a         2202 c         2006 g          682 t
ORIGIN      
        1 cgggcaagac gacgctgctg cggacggtcg ccggggagct ggcgccggcg gcgggcagca
       61 tcgcgcgtgg tccgcggggt gacgtcgcag gcgcccggtc aggccccggg acgcaccgcg
      121 ccggtcaccg cccaccggtc cgagcgggcc cgtcgcccta cggccacgag acggatcaac
      181 atgaacgcca tcaggcccgc ccagatccct gtcaggcccc acccccacgc gagcgatgcc
      241 cagatcagcg gcaggaatcc gacgaccgcg gcggccagcg tggacgtccg caggtaggcg
      301 gcgtctccgg caccgagcag caccccgtcc aaggcgaaca cgaccccggc cacgggctgc
      361 atggccacga agaaccacca gggcaccggt accgcggcga gcacctcggg atccggcgtg
      421 aacaccggcg gcagcacccc ggagagcgcg gcgaacagcg ccccgaaccc gaggccgagc
      481 acgatcccgt agcgcgtgac ctgcccggcc agggccctgg cccgctcggc gccccgctcg
      541 ggcgagccga gcgccgcgcc cacgagcgac tgcgccgcaa tcgccacggc gtcgagcacc
      601 agggcctgga agaaccacag ctgcaggacg atctggtggg ccgccgccgt cgcggcccca
      661 aatcgggtgg ccaccgcagc ggcggacagg aagcaggcct ggaacgcgag gctgcgggcc
      721 atcaggtcgc gcccgagccc gagctgcgac cgcagcacgg aaggcgcagg ccgcagcgcc
      781 accccgtcgg ccctggcggc ccgaacgagc gcgccgagga acaggctcgc gccgaccgtt
      841 tgaccgacga cgttggcgac cgccgacccg acgagtccga ggcccgccca cccgcccacc
      901 ccgtgcacga gcaggatgca ggccagcgcg gacagcccgt tgccggcgag gacgaagcgc
      961 aacggccggg ccgtgtcctg gacaccgcgc atccagccgt tgccggccag cgtcaccagc
     1021 acgagcggcg ctccgcacaa cgcgatccgc agccaggaca cagccgcatc ggcgatcgct
     1081 ccgccgttgc cgagcgccgc ggcgaccggc cgcgcgacga gctgaccgac cacgagcacc
     1141 agcagcccga cggcgagcgc gagccaggtg gcctgcaccc cctcggccac ggcggcgccg
     1201 cgcctgcccg ccccgtgcag ccgcgccgcg cgagccgtgg tgccgtagga caggaaattc
     1261 agctggctcg tgacctgcgc gaacaccacg cctgcgacgg cgaggccggc cagcggcaca
     1321 gcacccagcc tgccgaccac cgcggtgtcg acgagcaggt acagcggctc ggcggccagc
     1381 accggcaacg ccggagctgc cagcctcagc actgtccggg ccggcacgcg atcgggcggc
     1441 tgggtcatcg ggaacgggtc atgggcactg ctcacccgtc ggcgcgcggc accgcatgcg
     1501 gacggcggca cacgtagacg aacgcgggcg gcaggttgcg ccacacggtg gcggtgacca
     1561 gcacctcgtc gaacgtctcg cgcagggtgc ggcggaagcg tcgagccgcg ccgagcgtca
     1621 tgccgtgcag gtaggcgaag gtggtgaagg caccggtcgc gccgatcgcg cggctgatct
     1681 cgcccagaag cgctgtctgc gtcgcgtcgt cgaacagcgc ccatggcagc ccgcagatca
     1741 cggcgtcgac ggaggtgacc ccgcgcaggg cgagcagcgc gcccaggtcc gccgcgttgc
     1801 cgggcacgac ctccagctcc gggcgggtcc ggcgcaggaa cgcgaccatg tcggagtcca
     1861 gctcgacggc gaggtgccgg gcaccggccg gtagcttcgt ggcgatcacg gcgctcaccg
     1921 caccggtacc cgggccgagc tccacgacga cgggggaacc gcaccgcggt acgaccgacg
     1981 cgagcaccgc ccccagccgc ggcgagctgg gcgcgaccgc gcccatcgtc gcgggcctgc
     2041 gcagggcagc gacgaggaag gcccgtcggg ccgccctcgc atccggctcg atacgggttg
     2101 gtccggtatc ggacgtcatc tggctgggct cctcggtctc ggtcgctgca caccgtagcc
     2161 ggacgggcag cagggccgat caaccggcga ggtccgggga ttgactgctg catcgacctc
     2221 ttgcatcgac ctattgcgga gtacccggcg ggggcaggcg catcgtgcgc tgcggttcca
     2281 ccccggccac gccgtcgagg tcggccaacg cctcccaacc ggacggcgcc gatccggtga
     2341 tcacgccgac cggcgccagt acctgctcga cgtccatccc ggccgcgcgc agccgcgcgg
     2401 ccagctcgtc gatcgcggcg agatgggcgt cgtcgacggt gacggtgacg ggaatggggc
     2461 cttcgctgtg ggaagcgttc acgacggagc ctgcaccagc ccggcgccga catcctgcgc
     2521 gggcggtccg agcggctggg cggcgccgac gagctcgtcc cacagcgccc tgccgcgggc
     2581 cccgctgcgc tccgcccaca gcgcggcgat cccggcgacg tgcggggtgg ccatgctggt
     2641 gcccgagatc gtgttgtagc gccgcggcaa cggccagctg gagtgaacct ggacacccgg
     2701 cgcggcgatg tcgatctcgc caccgccgac ctccgtggcc gaggcggaga agtcggccac
     2761 cgccagggtg gcgtccaccg ctcctaccgc catgatcgac gggctgttcg ccggcactcc
     2821 gacgaacccg gggtcgccct ccgaccggct cgcgttgttc ccggcggccg ccacgacgag
     2881 cgtcccggcc gcgagcgcac ggcgccccac cgtctcgtag gcggtggaga tctcccgctg
     2941 gtccgcaccg agggacatcg agatcacctg cgcacccgcg ctgatggccc agctcatccc
     3001 ggcgaggatg tcggcgtcgg tgccgctgcc ctggtcgctc agcaccttgc cgaccagcag
     3061 ccgggccccg ttggccaccc cgtagcgcgg cccctcggcc gggtcgacgg ggcccgccat
     3121 cgtcccagcg cagtgcgtcc cgtgtccctg gacgtcctgc gcggtctggc cgtcgacgaa
     3181 cgaccgtgac gtgatgtcgc ggtcggcgaa gtcgggatgg cgcaggtcga tgccggtgtc
     3241 gagcaccgcg acgctgatcc ctgccccgga ttccgccgcc gcgagcacgc ccgtggcctg
     3301 cagcccccag gtggcctctg cgtcgtcgat gaacgggctg tcctgcaggg cgtcgaccgg
     3361 aacggcgtcc tgcagggcgt gcacgatccg ctccggctcc acggcgatga gccggtcgtc
     3421 ggccggttcg aacagcccgg tcgcccccac ccggtccggc accactgtca ctacggcgac
     3481 cccgagctcg gcgaacagca gcgcgcccgg ccccgtctgc cgggcgtcga gcgcgccagg
     3541 ctgggcgtcc ctggtggaga cgacctcctg cacgccgctc acctcgcgta ccaccgcgcg
     3601 cgacgcctcg tcgtccccga acaggtcgtc attgatcacg agcaggtagc gcccggtggt
     3661 cgaggccgcc ggttcacggg aaggtcgcat catcggccca tcgtcgccga agatcgatcc
     3721 gctgtcagat cgccgtgccg ctcaggtggc agggcgggac gtcatgcggg tcgtttcgca
     3781 ggatcgagcg ctgccagtgc tccgcggagg gcggcgagaa cctcgtcggc cgtgccgtca
     3841 cgcgtgaaac ccgccgcggc ccggtgaccg cccccatcga gcagcccggc cgccgccgcc
     3901 acatcgacgc gccccttcga ccggagcgag gtgacccagc gccggtggcc cacctgcttc
     3961 acgaccgccg cgacctcggc ctccgccgtg gtccgcaaga cgtccacgat gctgtcgacc
     4021 tcctccgccc ggaagcgggt ggtgtcctcg aacgggacac atgcgtgcac cagacccagc
     4081 ccacccgccg catccggctc gagcacggcg ttctgcaaca cctcacccag cgtggccagc
     4141 cacacgtagg ggtgcgtatc catgatcgga cgcaccaact cttcgggatc gacgcccgtg
     4201 gcgacgaggt cggcggccat gcggtgcgct gcctcccccg ccgtccggaa accacgggtg
     4261 tcggtgacca accccgcgta gaggcaccgg gcgacgtcgg cgtcgagcgg ctcccccatc
     4321 gcgacgagca cgcggtgggc cagcaccacc gtggcctcga cgccggggtc cagcacctgc
     4381 acatcgccga agcccgggtt ggacacgtga tggtcgatca tcaccgacga acccgcagtg
     4441 gccaagcggt ccgcaagcgc accgagtcgg gacggctccg cggcatcgca gaccaccagc
     4501 agctcaggag caaccggtac ctgctccggc ccgaccacca accccagcac gtccaacggg
     4561 acgagggtct cgggcacctg ctccggcgcc gcgaacgaca cccggaccgc agcgccatgc
     4621 cgccgcagca cgatgcccaa cgccagcgcg ctaccgagcg agtcggcgtc ggggttcaca
     4681 tgggccagga gcgtgacgtc acgcgcacgc atcagcagtc cggccgcggc cgcgaccgcg
     4741 gcgtcgaggg agccgcccat cccgaccccg ttcccgcgag cccgacctgt cactcctcgt
     4801 cggcccgctc acgcggagtg cggtacggat cgggatcacc ggcgggacgg gcacctgccg
     4861 cgagccgcgc cacctccgca tcggactccc gcgtgcgggc gagaagctcc tccatgcggc
     4921 gggcctcgtc cggcacctgg tcgggcacga acgtgagcga gggcgtgtgc cggataccgg
     4981 tgcccgcccc cacgagggag cgcaggacgc cggtggcgct cgccagtgcg gctgcagcgc
     5041 cggcggtgtc cgggtcggcg tcgaccgtct ccccgatcac cgtgtagtac agcgtggcct
     5101 cgcggaggtc accggtgacc cgggtgtccg tgatcgtcac catcttcagg cgaggatcct
     5161 tgacctcgtg ctccagggca gcggccacga tctgcgagat ccgcttggcc agccgccgcg
     5221 cgcgggcctg gtccaccatc acgtgctcct ctaggtctta cgagtcgtcg tcagggccca
     5281 gcatgcgctg ccgcgccgag agcagttcga gctccgggcg ggcggcaacg agccgttcgc
     5341 accgatcgag cacctcggtg acgtgaccgt ggtcggcggc gacgcagctg accccgagca
     5401 gcgcgcgccg atgcaggtcg aggtggcccg cctcggcgac ggccacctcg aacttgcggc
     5461 gcagctccgc cagaacgggc cggaccaccg accgcttctg cttgagggaa tggatgtccc
     5521 ccagcaggac gtccagctcc agcgctccga cgaacatggg tctccacgat cttcctgggt
     5581 gagacgccgg tggggggact ggccgaagcc ggtcccccca cgtcgcgtca gctcctcggc
     5641 ttctcgcgca tctcgaaggt ctcgatgacg tcgccgagct tgaggtcgtt gaaactgccg
     5701 agcgtgagac cacactcgaa gccctctcgg acctccaccg cgtcgtcctt gaaccg
//
LOCUS       DASRSC010000352         3100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1132072,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000352 DASRSC010000000
VERSION     DASRSC010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1132072"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFE39_17025"
     CDS             complement(<1..338)
                     /locus_tag="VFE39_17025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6260342.1"
                     /translation="MPQFRIADAARLLGVSDDTVRRWIEAGALPVQPDASNRKVIDGA
                     ALAAFARDHAHAPPDPSDVQSSARNRFVGLVTSVLTDKVMAQVEMQCGPHRVVSLMSS
                     EAVRELGLEPG"
     gene            453..1613
                     /locus_tag="VFE39_17030"
     CDS             453..1613
                     /locus_tag="VFE39_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HET6260343.1"
                     /translation="MRSFLDEKVLPAEAEYDAFRTERRGTPQEWDLPPVVEELKAEAR
                     ARGLWNLFLPSVSGLSNLEYAAVAEVSGWSPVIAPEAINCQAPDTGNMETLHLFGTPE
                     QKEQWLTPLLEGEIRSAFAMTEPDVASSDATNVQTTIRRDGDEYVISGRKWWISGSAD
                     ERCKIFIVMGKTDPDAASHRQQSMVLVPRDTPGLEIKRHLPTFGYQDQHGHSELAFHD
                     ARVPVTNLLGEEGGGFAIAQARLGPGRIHHCMRLIGMAERAIDLMVRRAQSRVAFGRP
                     LADQGVVREQIAQSRIDVEQARLLVLKTAWLIDRYGSKGAASEIAAIKVVCPRIACAV
                     IDRAIQIHGGAGMSDDVPLAYFYVWARALRLADGPDEVHLRAVARQELGRHN"
     gene            1827..2888
                     /gene="moaA"
                     /locus_tag="VFE39_17035"
     CDS             1827..2888
                     /gene="moaA"
                     /locus_tag="VFE39_17035"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HET6260344.1"
                     /translation="MTASELGLPAVRRPISRDPARPADPRLVDTFGRVATDLRISLTD
                     RCNLRCSYCMPAEGLDWMPREEQLTDDELLRLITIALRDLGIQELRFTGGEPLLRRGL
                     EGLIAASAALQPRPDISLTTNGIGLARRAESLAEAGVNRLNVSMDTLQQERFATITRR
                     DRLSDVLDGLAAARAAGLGPVKINTVLLRGVNDDEAVPLLRFAVDDGYELRFIEQMPL
                     DAQHGWERSEMITAGEIMVRLRESFDLTPDPTERGGAPAERWLVDGGPAVVGVIASVT
                     RPFCGACDRTRLTADGQVRSCLFARTETDLRAMLRGGSTDAEIADTWRHAMWGKLAGH
                     GIDDPGFLQPDRPMSAIGG"
     gene            2903..>3100
                     /locus_tag="VFE39_17040"
     CDS             2903..>3100
                     /locus_tag="VFE39_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HET6260345.1"
                     /translation="MSTTTIRATVRYFAAARAAAGAEAEQVELPTDGTVEDLLGTIRD
                     RHGPELARVLERCTFLLDEVAV"
BASE COUNT          463 a         1118 c         1061 g          458 t
ORIGIN      
        1 cccggctcga ggcccagctc ccgcactgcc tcgctggaca tcagggacac cacccggtgc
       61 ggcccgcact gcatctccac ctgcgccatc accttgtcgg tcaggaccga cgtgaccagc
      121 ccgacaaagc ggttgcgcgc cgagctctgg acgtcggagg gatcgggcgg ggcgtgagca
      181 tggtcgcggg cgaacgcggc gagcgcggcc ccgtcgatga ccttgcggtt cgacgcgtcc
      241 ggctgcacgg ggagcgctcc ggcctcgatc caccggcgga ccgtgtcatc gctgactccc
      301 agcaaccgcg cggcgtcggc gatcctgaat tgcggcacaa aggggatcct agagccgcga
      361 atgcgacatc gcccagcgcg ggctcctgct gtggttggct gcgtgtcgtg gacttcgaac
      421 ccagcgccgt cgctgccgac ctcaccaaac gcatgcgttc gttcctcgac gagaaggtct
      481 tgcctgccga ggcggagtac gacgccttcc gcaccgagcg ccgcggcacc ccgcaggagt
      541 gggaccttcc cccggtcgtc gaggagctca aggccgaggc acgcgcgcgc gggctgtgga
      601 acctgttcct gccgagcgtc tccgggctgt ccaacctcga gtacgctgcg gtggccgagg
      661 tctccggctg gtcgccggtc atcgctcccg aagcgatcaa ctgccaggcc ccggacaccg
      721 ggaacatgga gaccctgcac ctgttcggga cacccgagca gaaggagcag tggctcaccc
      781 cgctgctcga gggcgagatc cgctcggcct tcgcgatgac cgagcccgac gtcgcgtcct
      841 ccgacgccac caacgtccag accacgatcc gccgcgacgg cgacgagtac gtcatctccg
      901 ggcggaagtg gtggatctcc gggtcggccg acgagcgctg caaaatcttc atcgtcatgg
      961 gcaagaccga cccggacgcc gcgtcgcacc ggcagcagtc catggtgctc gtaccccggg
     1021 acaccccggg cctggagatc aagcgccacc tgccgacgtt cggctaccag gaccagcacg
     1081 gacactcgga gctggccttc cacgacgcac gcgtgccggt gacgaacctg ctcggcgagg
     1141 agggcggcgg cttcgcgatc gcgcaggctc ggctcggccc cggccgcatc caccactgca
     1201 tgcggctgat cgggatggcg gagcgggcca tcgatctcat ggtgcggcgc gcgcagtccc
     1261 gtgtggcgtt cggccggccg ctggcggacc agggtgtggt gcgcgagcag atcgcccagt
     1321 cacgcatcga cgtggagcag gccaggctgc tcgtgctcaa gacagcctgg ctgatcgaca
     1381 ggtacggctc gaagggcgcg gcctccgaga tcgcagcgat caaggtcgtg tgtccccgga
     1441 tcgcgtgtgc ggtgatcgac cgcgcgatcc agatccacgg tggcgccggg atgagcgacg
     1501 acgtgccgct ggcctacttc tacgtgtggg cgcgtgccct tcggctggcc gacggtcccg
     1561 acgaggtgca cctgcgcgcc gtcgcccgcc aggagctggg ccggcacaac tgaccaccac
     1621 tgcaccgggc gagttcgggt gagcaagggg ttcgtgatcg gtccgatccg atcagcagcg
     1681 gcgccgctga cctttcgggc agcagtacac gctcgtaggt tcgcagaagc gggctgagtc
     1741 cacgatttgc tccgcagttg cggattcctg tccaggtgca gatcacgacc cgtttctgac
     1801 ctgtctggga cggttacgct catggtgtga cggcatccga gctcggcctc cccgcagtgc
     1861 ggcggcccat ttcccgagac ccggcccgcc ctgccgaccc ccggctggtc gacaccttcg
     1921 gtcgggtcgc cacggacctg cggatctccc tgaccgaccg gtgcaacctg cgctgctcct
     1981 actgcatgcc cgcggagggc ctcgactgga tgccgcgcga ggagcagctc accgacgacg
     2041 agctgctccg gctgatcact atcgccctgc gcgacctcgg tatccaggag ctgcgcttca
     2101 ccggcggcga gccactgttg cggcggggcc tcgaaggtct gatcgcggcg tccgcggcgc
     2161 tgcagcctcg tccggacatc tcgctcacca ccaacggcat cgggctcgcc cgccgggcgg
     2221 aatcgctcgc cgaggccggg gtcaaccggc tgaacgtctc gatggacacg ctgcaacagg
     2281 agcggttcgc caccatcacc cggcgcgacc ggctctccga cgtgctggat ggcctcgccg
     2341 ccgcccgcgc ggccgggctc ggcccggtca agatcaacac ggtgctgctg cgcggcgtga
     2401 acgacgacga ggcggtcccg ctcctgcgct tcgccgtcga cgacggttac gagctgcggt
     2461 tcatcgaaca gatgccgctc gacgcccagc acggctggga gcgttccgag atgatcacgg
     2521 cgggcgagat catggtgagg ctccgcgaga gcttcgacct gacgcccgac cccaccgagc
     2581 gcggcggtgc ccccgccgaa cgctggctgg tggacggcgg ccccgccgtg gtcggcgtca
     2641 tcgcgtcggt gacgcgcccc ttctgcggcg cctgcgaccg cacccggctc accgccgacg
     2701 gacaggtccg ctcgtgcctg ttcgcgcgca cggagaccga cctgcgggcg atgctgcgcg
     2761 gcggttcgac cgatgccgag atcgccgaca cctggcgcca cgccatgtgg ggcaagctcg
     2821 ccggccacgg catcgacgac cccggcttcc tgcagccgga ccgcccgatg agtgcgatcg
     2881 gcgggtgaga aacatgtccg ggatgagcac caccaccatc cgcgccaccg tgcgctactt
     2941 cgctgcggcc cgcgcagccg ccggcgcgga ggcggagcag gtggagctgc cgacagacgg
     3001 caccgtcgag gaccttctcg gcaccatccg cgaccggcac ggccccgagc tggcccgtgt
     3061 gttggagcgg tgcaccttcc tgctcgacga ggtcgcggtc
//
LOCUS       DASRSC010000353        10935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5554529,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000353 DASRSC010000000
VERSION     DASRSC010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10935
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5554529"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="VFE39_17045"
     CDS             complement(<1..1442)
                     /locus_tag="VFE39_17045"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HET6260346.1"
                     /translation="MVADSPFVSDVPAAAALAAWGEGCTAAGCPARLEVVRLPIGEAV
                     GRVTAEPVWARRSSPAFDSAGMDGIAVRAADTVGAAETTPVLLDDFAVVDTGDPLPEG
                     YDAVVMREHVHRGGNGTAEVRTAVPPYQHVRSIGEDITATELLLPAGHRLRPVDVAAA
                     AAAGAVELAVRRAPVVVIVPTGDEIRPIGTDPAPGEILDTNSLMLAAQARELGCEARV
                     SPIVRDDPAVITEALRDAAAEADLVVLVAGSSAGRDDYTARVVAGAGTLMVHGVAVRP
                     GHPVVLGAVDATPVLGAPGYPVSAALTFDIFAAPLLAALEGAAPRERPSTTARLARKL
                     ASNVGMDDWVRVRLGRVGGDVVATPLPRGAGVLTSLVRADGLLVVPAGLEGHHAGERV
                     RVELLRGLAEIGRTIVAIGSHDLVLDMAASALRADDPLVTLASSNVGSLGGLVALRDG
                     LCHIAGSHLLDPATGEYTLPYVDKILGGDDV"
     gene            1553..2146
                     /locus_tag="VFE39_17050"
     CDS             1553..2146
                     /locus_tag="VFE39_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260347.1"
                     /translation="MCLVGITATLFDVNMTTTPSQQAHPAGYLSRWRERRRERLRLAR
                     QDRLAAQLAELHHIQTLIADARTLVASGWVQDGWFTYRDERGQQCTVRGMNLQGMADR
                     PVTGACLVGAIAHAGGGLAALRTAPVQGALDVTWQTLHRDQHEPGHWCPVPTVRTNHV
                     RDLTRWNDHSSRLPDEVTALLHTAEQAAAAQIERLRA"
     gene            complement(2166..3113)
                     /locus_tag="VFE39_17055"
     CDS             complement(2166..3113)
                     /locus_tag="VFE39_17055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET6260348.1"
                     /translation="MSASAPSMLVIAVPAAVVGAASFGLASALQQRATKEVPTSRTLN
                     PGLLVQLAHRRDWLAATAAVGLGLALQVVALAFAPLTLVQPLLVTGVLFGTLFAARLA
                     HRPLDRQILLGALASVVGLAAFLALARPSGGSTVLPGLPTLLPLAVILAAIVAGCVVL
                     SSLPRVSPTVQVGALALATGICYGVTAGLMKVVTGQIRDGGLAEIFGHPAFYVVCVVG
                     PMGFLLSQNTFQHGLLIAPALATITIVDPLVGIAIGVAWLGEKLDSSPPVLVGEAISA
                     VVVIAGIAVLAHRGTQLRHVIEDQLQTGDQTGTAPPTAG"
     gene            complement(3167..3652)
                     /locus_tag="VFE39_17060"
     CDS             complement(3167..3652)
                     /locus_tag="VFE39_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260349.1"
                     /translation="MTHNVLAGLAAGAAGTTALDAVSYLDMVLRARPPSSTPEDTVRR
                     LEEALPLRLHPEGRDSEAASNRRQGLGALLGIAAGLAVGAGYGLVRPRLGRLPLAVLG
                     TGAGLAANIGTTGPMVAMGVTDPRSWPVSSWLSDLLPHLAYGFATAAVFELTCHARRS
                     A"
     gene            complement(3698..4930)
                     /gene="glp"
                     /locus_tag="VFE39_17065"
     CDS             complement(3698..4930)
                     /gene="glp"
                     /locus_tag="VFE39_17065"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HET6260350.1"
                     /translation="MSGREFFATRTVAEALAGFRPARRTVLEDVALDGALHRVPATDV
                     AAVAALPGFARSTVDGYAVRAADTYGASEGLPSYLDLVGAVRMGTAPTVAVRPGGCVA
                     MPTGGVLPEGADAVVMVEHTAETMPGTIEVTRPVAPLEGLVRADEDVAAGAPLVPGGR
                     PLRAPDLGLLAAAGVTTVRVHARPRVVIVSTGDEVVPPSTAQLCPGQVRDATASALAG
                     LVRDAGGEPVLAGIVPDDAGALEATLRDALRDADLVVVSAGSSVGARDETAGAVAALG
                     DIWCHGLALKPGKPTLLAECGSDSGNVPLIGLPGNPLSALVVFRLVGVPLVWRLAGCP
                     VPPPEPSTCARLARDLPSAAGRLDVVQVTVRDGTATPIFGPSALLSVLTRADGCLVVP
                     EPATGLAAGSEVQVTLYR"
     gene            complement(4927..6684)
                     /locus_tag="VFE39_17070"
     CDS             complement(4927..6684)
                     /locus_tag="VFE39_17070"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HET6260351.1"
                     /translation="MTELSDRMGEVRSLRVPGVEARAGKFPGPSLIPALNAIQARLGW
                     LPREELEELAREARRPRYEIEGLISFYPHFRTEPPAKVALHACHDLPCWLRGGEERLA
                     ELRDRYSADSDVELVEVSCLGRCDIAPAVAVNERPAPVADADDLVDAAKGSGVGDAAA
                     ATRSEPWPNDPYPAGSGVEDRYATLRALLAGKLDADEIVSMLKDSGLRGMGGAGFPTG
                     QKWGLVRDAQPGSTKYAIVNADESEPGTFKDRQILAQQPHLVLEGLLLGMAVVGAEEG
                     WVFIRHEYGPEEHVLREEIDALRAAGVVGPDACGSGRRLSVDIFVSPGGYILGEETAL
                     LECMEGHRGEPRNKPPFPGNYGLHGRPTLINSVETFADVPVIVQRGAQWWKDQGAGDS
                     VGWKFFAVSGHVERPDVYCVPMGTTVRELIDLAGGVTGGAAVGAVQPGGASSNFIGPD
                     QLDLRLDFGTAAEAGTMLGSGALVVLAEGTDLLAASTNVLRFFRNESCGKCVPCRVGS
                     TKAHELLRDVLASGNSTLDDAQRGRILQLEEAMRKTSICGLGQVALGPVVSVLGLDKG
                     GAAAREQPRPNGAAAQPDR"
     gene            complement(6681..9659)
                     /gene="fdhF"
                     /locus_tag="VFE39_17075"
     CDS             complement(6681..9659)
                     /gene="fdhF"
                     /locus_tag="VFE39_17075"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HET6260352.1"
                     /translation="MTAVQEPTTAVRTVTATIDGRETTVAEGTSIYDAAKQVGVDIPV
                     LCHNERYDPVGVCRMCVVDTGGRVFAAACVRPCEDGMEVTTSSPELERNRATLTELLV
                     SDQPPRAEDPKQTTTGDNLLLALADGFGVARETTDLPCGSGRGVDSSNPVIDVNHDAC
                     ILCDRCVRACDDIQGNDVIGRSGKGYSTRIAFDLNDPMGASSCVTCGECVQACPTGAL
                     TNKPIRDIPIRPREELDAVDSVCPYCGVGCALTYYVDRERGAIAFAEGRDQPGSQSRL
                     CVKGRYGWDYAASPQRITVPLIRVDAAYPKGPLSADVRGEGRGSANGATNDRGRGGNG
                     GNRSGGGKDDPGGKRGGGRKPGGLVDYDEVLPHFREATWEEALDLVARRLKEIHAEGG
                     PGAIAGFGSAKCSNEEAYLFQKLIRTGFGTNNVDHCTRLCHASSVAALFEGVGSGAVS
                     TTYGDVANADVVIITGSNPTANHPVASSFFKQARRRGTKIIYVDPRASTVAEHSDIFV
                     QLKPGTDVAFYNSVMHEVIRLGLMDREFIANRTSNYDELARTVADYPPERAAQITGVD
                     ADQIREVARTWGEANAAVVYWGMGISQHTTGTDNARCLIALCSITGNVGRPGTGLHPL
                     RGQNNVQGASDAGLIPMFYPDYQGVDLDGTRQHFEQAWGTTLNPNRGLTVTEIIKSVL
                     QPGGVRGMYMLGENPFLSDPNINKVRKALSALDFLVVQDIFLTETAEFADVILPATSY
                     LEKDGTYTNTDRRVQLGRKVMDPPGQARADWQIVQDIAVRIGLDWAYATPSEVFDEMV
                     ALMPSYKNLSYDNLGLSGKLYPNADPEHSDGTIVMFDEKFNTDDGLAHLVPAQWLPAR
                     ELPDAEFPLVLNTGRLLEHWHTGSMTRRSYALDAISPVAEVYLHPKDAADRGLAHGEM
                     VRVRSRRGEIELLVRVSHREQLGNCFIPFHFREAAANLLTIDEVDPTGKIPEFKFCAV
                     QVEALGAAHAETTSTAGVRA"
     gene            complement(9838..10410)
                     /locus_tag="VFE39_17080"
     CDS             complement(9838..10410)
                     /locus_tag="VFE39_17080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260353.1"
                     /translation="MFSPSARANDVEVAGSAGPADPARRERRRVPGGPRPGRQPALHA
                     PTVDGAEHSLRTTTDGFDKCNTFAQGYVDLVGSWVTDVAAGRPAPTTVAGAGVQDRPT
                     SAVPPLAWYESVWVHAGALAVLLVGFGGFGLDAAWRRLRGRAARRAPWPARVLAVSAG
                     TTGDRARAGLLIAAGAVFVPWAVYLGLLLP"
     gene            10385..10825
                     /locus_tag="VFE39_17085"
     CDS             10385..10825
                     /locus_tag="VFE39_17085"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HET6260354.1"
                     /translation="MARALGENTGATSYHLRQLAEHGFVEEVPERGRGRERWWRAAVR
                     DIRFPPRSRMDEEVRAAFDELGRLNMADDVAAFARFQQQRDSIGEWGDALLFARGALN
                     LTVPELRRFWEDYMALYERHARAAASPTQGARRVLVRFVAFPEV"
BASE COUNT         1574 a         3930 c         3975 g         1456 t
ORIGIN      
        1 acatcgtcac caccgaggat cttgtccacg tagggcagcg tgtactcccc ggtggccgga
       61 tccagcaggt gcgagccggc gatgtgacag agcccgtcgc gcagcgccac gaggccaccc
      121 agcgacccga cgttggacga ggccagcgtc accagcgggt cgtcggcccg cagcgcggac
      181 gcggccatgt cgagcaccag gtcgtgcgag ccgattgcca cgatcgtgcg gccgatctcg
      241 gcgagcccgc gcagcagctc cacccgcacc cgttcgccgg cgtggtggcc ctccagcccg
      301 gccgggacga ccagtagccc gtccgcgcgc accaacgatg tgagcacgcc ggcgccgcgg
      361 ggcagcggcg tcgccaccac gtcgccgccg acccggccca gccgcacccg cacccagtcg
      421 tccatcccga cgttggaggc gagcttgcgg gcgagccgcg ccgttgtcga gggccgttcc
      481 cgcggcgccg cgccctcgag tgcggcgagc agtggcgcag cgaagatgtc gaacgtgagc
      541 gcggcggaca ccgggtagcc gggggcgccg agcacgggag tggcgtcgac ggccccgagc
      601 accaccgggt gtccgggccg gacggccacc ccgtgcacca tgagcgtgcc ggctcctgcg
      661 acgacccgcg cggtgtagtc gtcgcggccc gcgctggacc cggcgacgag caccaccagg
      721 tcggcctccg ccgcggcgtc ccgcagggct tcggtgatca cagccgggtc gtcgcggacg
      781 atcggcgata cccgcgcctc gcagcccagc tcacgcgcct gcgccgcgag catcagggag
      841 ttcgtgtcga ggatctcgcc gggggcggga tcggtgccga tcggacggat ctcgtcgccg
      901 gtcgggacga tcaccacgac cggggcacgc cggacggcga gctcgacggc gcctgccgcg
      961 gcggccgcgg ccacgtcgac cggacgcagc cggtggccgg ccgggagcag cagctcggtg
     1021 gcggtgatgt cctcgccgat ggaacgcacg tgctggtacg gcggcaccgc ggtgcgcacc
     1081 tcggccgtgc cgttgccgcc gcggtgcacg tgctcgcgca tgacgacggc gtcatagccc
     1141 tcgggcagcg ggtctccggt gtcgaccacg gcgaagtcgt cgagcagcac cggggtggtc
     1201 tccgccgcgc ccaccgtgtc ggccgcgcgg accgcgatgc catccatgcc ggcggagtcg
     1261 aacgcggggg aggagcgccg cgcccacacc ggttccgcgg tcacccggcc gacggcctcg
     1321 ccgatgggca gccgcaccac ctccaggcgc gccgggcagc cggcggcggt gcacccctcg
     1381 ccccacgcgg cgagcgccgc tgcggcgggg acgtcggata caaacggaga gtcggcgacc
     1441 acgtgggcga cggtacgacc gggcaggacg aggtggacat cggggagggg tacctgccag
     1501 caggagtccg agcactccgg tcagcaccgg agcgtgagcc acgctggcgg tgatgtgcct
     1561 ggtgggcatc accgcgacgc tgttcgacgt gaacatgacc accacaccct cccagcaggc
     1621 tcacccggcc gggtatctca gccgatggcg ggagcgcagg cgggaacggc tgcgtctggc
     1681 ccgtcaggat cgcctcgccg cgcagctggc agagcttcac cacatccaga ccctcatcgc
     1741 tgacgcccgc accctcgtcg cctccggctg ggtccaggat ggctggttca cctaccgcga
     1801 cgagcgggga cagcagtgca ccgtcagagg catgaacctg caggggatgg cggacaggcc
     1861 ggtcaccggg gcctgcctgg tcggggcgat cgcgcatgcc ggcggcgggc tggcggcact
     1921 tcgcaccgca cccgtgcagg gagcgctgga cgtgacgtgg cagacgctcc accgcgacca
     1981 gcacgagccg ggccactggt gccccgtccc caccgttcgc accaaccacg tccgcgacct
     2041 caccaggtgg aacgaccact ccagccgcct ccccgacgag gtgaccgcac tgctccacac
     2101 cgccgagcag gccgcggcag cgcagatcga acgactccgc gcgtaaggcg gctttcgtcg
     2161 atgcctcacc cggcggtcgg cggcgcggtc cccgtctggt cccccgtctg gagctggtct
     2221 tcgatgacgt gtcgcagctg tgtgccgcgg tgggcgagca cggcgatacc ggctatcacg
     2281 accacggccg agatcgcctc gccgacgagc acgggcggcg agctgtcgag cttctcaccc
     2341 agccaggcca cgccgatcgc gatcccgacc agcggatcca cgatcgttat cgtcgcgagg
     2401 gcgggcgcga tgaggagccc gtgctggaag gtgttctggc tgagcaggaa ccccatcggg
     2461 ccgaccacgc acaccacgta gaacgcgggg tgcccgaaga tctccgccag ccctccgtcg
     2521 cggatctgtc cggtcacgac cttcatcagg ccggcggtga ccccgtagca gataccggtg
     2581 gccagggcca gcgccccgac ctgcacggtg ggggacaccc gcggtagcga gctgaggacc
     2641 acacagcccg cgacgatcgc cgccaggatc accgcgagcg ggagcagcgt cggcagcccg
     2701 ggcaggaccg tcgagccgcc gctcggccgg gcgagggcca ggaacgcggc caacccgacc
     2761 accgaggcca gcgcgccgag gaggatctgg cggtccagag gacgatgtgc cagccgcgcg
     2821 gcgaagaggg tcccgaagag gacaccggtc accagcagcg gttggaccag cgtcagcggt
     2881 gcgaacgcga gggcgacaac ctggagcgcc agaccgaggc cgacggcggc cgtcgccgcg
     2941 agccagtccc gccggtgcgc gagctgcacc agcagccccg ggttcaacgt cctgctggtc
     3001 ggaacctcct tggtggcccg ctgctgcagc gcgctggcca gtccgaagct cgccgcaccg
     3061 accacagcgg cgggaaccgc gatgacgagc atcgacgggg ctgaggcgga cacgggacct
     3121 ccacgatgtg acgtctcacg ggtcgaacat cccaggcgcg cgaggtctag gccgaccggc
     3181 gggcgtggca ggtcagctcg aacacagccg ccgtggcgaa cccgtaggcc aggtgcggca
     3241 ggagatcgga gagccacgag ctcaccggcc agctccgcgg gtcggtgacg cccatcgcca
     3301 ccatcggccc ggtggtgccg atgttggcgg cgagcccggc tccggtgccg aggacggcga
     3361 gcgggaggcg gcccagccgc ggacgcacga gcccgtatcc ggcgccgacg gcgaggcccg
     3421 cggcgatccc cagcagagca ccgagcccct ggcggcggtt ggacgccgcc tcgctgtcgc
     3481 gaccttccgg gtgcaggcgc agcgggaggg cttcctcgag ccggcgcacg gtgtcctccg
     3541 gcgtcgagct gggcggtcgg gcgcgcagca ccatgtcgag gtagctgacc gcgtccaatg
     3601 cggtggtccc ggccgcgccc gcggcgagac cggccagcac gttgtgtgtc atgacccggg
     3661 gcttccccgt ctccccacgg ggtagacctg ctcggcgtca gcggtacagc gtgacctgca
     3721 cctcggaacc tgcggcgagc ccggtcgcag gctccggcac gacgaggcag ccgtcggcgc
     3781 gggtgagcac cgacaggagc gccgacgggc cgaagatcgg tgtcgccgtc ccgtcgcgca
     3841 cggtgacctg cacgacgtcc aacctgcctg cggccgatgg cagatcgcgg gcgaggcggg
     3901 cgcaggtcga cggttccggc ggcgggaccg gacagcccgc cagccgccac accagcggca
     3961 cgccgaccag ccggaacacc acgagcgccg agagcgggtt gcccgggagc ccgatgaggg
     4021 ggacgttgcc gctgtccgac ccgcactccg cgagcagtgt cggcttgccg ggcttgaggg
     4081 cgagcccgtg acaccagatg tcgcccagcg cagccaccgc gcctgctgtc tcgtcgcgag
     4141 ctcccaccga cgaccctgcc gagaccacca cgaggtcggc gtcgcgcagc gcgtcgcgca
     4201 acgtggcctc cagcgcgccc gcgtcgtcgg gcacgatccc ggccagcacc ggctcgccgc
     4261 cggcatcgcg cacgagaccg gcgagtgccg aggccgtggc gtcacggacc tgccccgggc
     4321 acagctgtgc ggtggacggg ggcaccacct cgtcaccggt cgagacgatc accacccgcg
     4381 ggcgggcgtg tacccggacc gtggtgacgc cggcagcggc gagcagcccc aggtcggggg
     4441 cgcgcagcgg ccggcccccg ggcaccagcg gcgcccctgc cgcgacgtcc tcgtcggcgc
     4501 gtacgagccc ctcgagcggc gccacgggcc gcgtcacctc gatcgtgccg ggcatcgtct
     4561 cggcggtgtg ctccaccatg acgacggcat cggcaccctc gggcagaacc ccgccggtcg
     4621 gcatcgcgac gcatccgccc ggccgcacgg cgacggtcgg cgccgtaccc atgcggaccg
     4681 cccccaccag gtccaggtag gagggcaggc cctcggacgc gccgtaggtg tcggcggcgc
     4741 gtactgcgta gccgtcgacg gtggaccggg cgaagcccgg cagtgccgcc acggcggcca
     4801 cgtcggtggc cggaacgcgg tgcagagcgc cgtcgagcgc tacgtcctcc agcacggtgc
     4861 gccgggcggg ccggaacccc gccaacgcct cggccaccgt gcgggtggcg aagaactcgc
     4921 gtccgctcac ctgtcgggct gggccgccgc gccgttcggc ctgggctgct cccgcgcggc
     4981 cgcccctccc ttgtcgagac cgagcacgct caccaccggg ccgagcgcca cctgcccgag
     5041 cccacagatc gacgtcttgc gcatggcctc ctcgagttgc aggatccggc cgcgctgggc
     5101 gtcgtccagc gtggagttgc ccgacgccag tacgtcgcgg agcaactcgt gcgccttcgt
     5161 cgaccccacc cggcacggca cgcacttacc gcacgactcg ttacggaaga accgcagcac
     5221 gttggtcgac gccgcgagca ggtcggtgcc ctcggccagg acgacgagcg caccggatcc
     5281 gagcatggtg cccgcctcgg ccgccgtgcc gaagtcgagc cgcaggtcga gctggtcggg
     5341 gccgatgaag ttggacgacg caccgcccgg ctgcacggca ccgacggccg cgccgcccgt
     5401 gaccccaccg gcgaggtcga tcagctcgcg gaccgtggtg cccatcggca cgcagtacac
     5461 gtcgggacgt tcgacgtgcc cggacacggc gaagaacttc cagcccaccg agtcgcctgc
     5521 gccctggtcc ttccaccact gcgcaccgcg ctgcacgatc accggcacgt cggcgaacgt
     5581 ctccaccgag ttgatcagcg tgggcctgcc gtgcaggccg tagttgccgg ggaacggggg
     5641 cttgttgcgc ggctcgccgc ggtggccctc catgcactcc agcagcgcgg tctcctcacc
     5701 gaggatgtag ccgccggggg agacgaagat gtcgacgctg aggcgccgcc cgctcccgca
     5761 cgcgtccggc ccgaccacgc ccgctgcccg caacgcgtcg atctcctcgc gcagtacgtg
     5821 ctcttcgggg ccgtactcgt gccggatgaa gacccagccc tcctcggcgc cgaccacggc
     5881 catcccgagc agcaggccct ccagcacgag gtgtggctgc tgggccagga tctggcggtc
     5941 cttgaacgtg ccgggctcgg actcgtcggc gttgacgatg gcgtacttgg tgctccccgg
     6001 ctgggcgtcg cgaacgagcc cccacttctg gccggtgggg aagccggcgc cacccatccc
     6061 gcgcaggccg gagtccttca gcatggagac gatctcgtcg gcgtcgagct tccccgcgag
     6121 cagcgcacgt agcgtggcgt agcgatcctc gacgccagac ccggcggggt aggggtcgtt
     6181 gggccacggt tcgctacggg tggccgcggc ggcgtcgccc acgcccgacc ccttggcggc
     6241 gtcgacgagg tcgtcggcgt cggccacggg cgccgggcgc tcgttgaccg ccaccgcagg
     6301 cgcgatgtcg caccggccga ggcacgacac ctcgacgagc tcgacgtcgc tgtcggcgct
     6361 gtagcggtca cgcagctcgg cgagacgttc ctcgccgccg cgcagccagc acggcaggtc
     6421 gtggcaggcg tgcagcgcca ccttggccgg cggctccgtg cggaagtgcg ggtagaacga
     6481 gatcagtccc tcgatctcgt accgcggccg gcgggcctcc cgggcgagct cctccagctc
     6541 ctcgcgcggg agccaaccca gccgggcctg aatcgcgttc agtgccggga tgagtgacgg
     6601 tccggggaac ttcccggcac gcgcctccac acccgggacg cgcagtgagc ggacctcgcc
     6661 catgcggtcg gacagctcgg tcatgcacga actcctgcag tcgatgtcgt ctccgcatgt
     6721 gccgcgccga gggcctcgac ctgcaccgcg cagaacttga actcggggat cttgccggtg
     6781 gggtcgacct cgtcgatcgt caggaggttg gccgccgcct cgcggaagtg gaacgggatg
     6841 aagcagttgc ccagctgctc gcggtgcgag acgcgcacca gcagctcgat ctcgccgcgc
     6901 cgcgaccgca cccgcaccat ctccccgtgg gccagcccgc ggtcggcggc gtccttcggg
     6961 tgcaagtaca cctcggcaac cggtgagatc gcgtcgagcg cgtaggaccg ccgcgtcatc
     7021 gagccggtgt gccagtgctc gagcaacctg ccggtgttga gcaccagcgg gaactcggcg
     7081 tcgggaagct cccgtgccgg gagccactgc gccggcacga ggtgagcgag cccgtcgtcg
     7141 gtgttgaact tctcgtcgaa catgacgatg gtgccgtcgg agtgctccgg gtcggcgttc
     7201 gggtagagct tcccggacag accgaggttg tcgtacgaga ggttcttgta cgagggcatg
     7261 agcgccacca tctcgtcgaa cacctcgctc ggggtcgcat aggcccagtc caacccgatc
     7321 cggaccgcga tgtcctgcac gatctgccag tcggcgcggg cctggccggg cgggtccatc
     7381 accttgcggc cgagctgcac ccgccggtcg gtgttggtgt acgtgccgtc cttctcgagg
     7441 tacgaggtgg ccggcaggat cacgtcggcg aactcggcgg tctcggtgag gaagatgtcc
     7501 tggacgacga ggaagtccag cgccgagagc gccttgcgga ccttgttgat gttggggtcg
     7561 gagaggaatg ggttctcccc gagcatgtac atcccgcgca ccccgccggg ctgcagcacc
     7621 gacttgatga tctcggtgac ggtcaggccg cggttcgggt tgagggtggt gccccacgcc
     7681 tgctcgaagt gctgccgggt gccatcgagg tcgacgccct ggtagtcggg gtagaacatc
     7741 gggatcagcc cggcgtcgga cgcgccctgc acgttgttct gcccgcgcag cgggtgcaga
     7801 ccggtgcccg gacgcccgac gttgccggtg atcgagcaca gcgcgatcag gcagcgggcg
     7861 ttgtcggtgc cggtggtgtg ctgggagatc cccatccccc agtagacgac ggcggcgttc
     7921 gcctcgcccc acgtcctcgc gacctcgcgg atctggtcgg cgtcgacgcc ggtgatctgc
     7981 gcagcccgct ccggcgggta gtcggcgacg gtgcgcgcga gctcgtcgta gttcgacgtg
     8041 cggttcgcga tgaactcgcg gtccatcagc ccgagccgga tcacctcgtg catcaccgag
     8101 ttgtagaacg ccacgtcggt gcccggcttg agctgcacga agatgtcgga gtgctcggcc
     8161 acggtggacg cgcgcgggtc aacgtagatg atcttcgtgc cgcgccgcct ggcctgcttg
     8221 aagaacgagc tggccacggg gtggttggcc gtcgggttgg agccggtgat gatcacgacg
     8281 tcggcgttgg cgacgtcgcc gtaggtggtg gacaccgcgc cggagccgac gccctcgaac
     8341 agcgccgcca cggaggacgc gtggcacagc cgggtgcagt ggtcgacgtt gttggtgccg
     8401 aagccggtgc ggatcagctt ctggaagagg taggcctcct cgttggagca cttcgccgac
     8461 ccgaacccgg cgatcgcgcc ggggccgccc tcggcgtgga tctccttcag gcgacgggcg
     8521 acgaggtcga gcgcctcctc ccaggtggcc tcccggaagt gcggcaacac ctcgtcgtag
     8581 tcgacgaggc cgccgggctt gcggcccccg ccccgcttgc cgccggggtc gtccttgccg
     8641 ccgccgctgc ggttgccgcc gttgccgccg cggccccggt cgttggtggc gccgttggcc
     8701 gagccgcggc cttcgccgcg cacatcggcc gacagcgggc ccttggggta ggcggcatcg
     8761 acgcggatca acggcaccgt gatccgctgc ggggaagcgg cgtagtccca gccgtagcgg
     8821 cccttgacgc agagcctgct ctgcgagccg ggctggtcgc ggccctcggc gaaggcgatc
     8881 gcgccgcgct cgcggtcgac gtagtaggtg agcgcacagc cgacaccgca gtaggggcac
     8941 accgagtcga cggcgtcgag ctcctcgcgc gggcggatcg ggatgtcgcg gatcggcttg
     9001 ttggtgagcg ctccggtcgg gcaggcctgg acgcactctc cgcaggtgac gcaggagctc
     9061 gcgcccatcg ggtcgttgag gtcgaaggcg atgcgggtgg agtagccctt gccggagcgg
     9121 ccgatcacgt cgttgccctg gatgtcgtcg caggcacgga cgcaacggtc gcagaggatg
     9181 caggcgtcgt ggttgacgtc gatcaccggg ttcgacgagt cgacaccgcg ccccgacccg
     9241 cacggcaggt cggtggtctc gcgggcgaca ccgaacccgt cggccagcgc cagcagcagg
     9301 ttgtcaccgg tggtcgtctg cttggggtcc tcggcgcgcg ggggctggtc ggacaccagc
     9361 agttcggtga gcgtggcccg gttgcgctcg agctccgggc tggaggtggt gacctccatg
     9421 ccgtcctcgc acggccgcac gcaggccgcg gcgaacacgc gccctccggt gtcgaccacg
     9481 cacatgcggc agacaccgac cgggtcgtat cgctcgttgt ggcagagcac cgggatgtcg
     9541 acgccgacct gtttggcggc gtcatagatg gacgttccct cggcgacggt ggtctcgcgg
     9601 ccgtcgatcg tggcggtgac cgtgcgtaca gcggtcgttg gttcctgcac ggctgtcatc
     9661 tcggcctccg gcggtcaggt tcgtgacggt actgacctta gtcgactgta tgcaatcgcg
     9721 tcagtctcgc gcagcagatc ggccagtgca ccggcggccg gttgctggga tgtgatcatc
     9781 agatcacccg atccggctgc gaacgcgcag cacgaagcct agtcctcgcc gcgaatctca
     9841 gggcaggagc aggcccaaat agacagccca cggcacgaag accgcgccgg ccgcgatcag
     9901 gagccctgcg cgcgcacggt ctccggtggt cccggccgac accgccagta cgcgggccgg
     9961 ccacggggca cggcgggcgg cccggccgcg cagccgccgc catgccgcgt ccaggccgaa
    10021 ccccccgaag ccgaccagca gtaccgccag cgcgccggca tggacccaga ccgactcgta
    10081 ccaggccagt ggcggcaccg ccgaagtcgg ccggtcctgc acgcccgcgc ccgccaccgt
    10141 ggtgggcgcc ggccgcccgg cggcgacgtc cgtgacccag gagccgacga ggtcgacata
    10201 gccctgcgcg aacgtgttgc acttgtcgaa gccgtcggtc gtcgtccgca gcgagtgctc
    10261 ggcgccgtcg acagtgggag cgtgtagtgc cggttgccgg cctggtcgag ggccgcccgg
    10321 tacgcggcga cgctctcgac gggcggggtc agccggtcct gccgatccag cgacctcgac
    10381 gtcattggcg cgggcactcg gcgagaacac gggggccacc agctaccacc tgcggcagct
    10441 cgccgaacac gggttcgtcg aggaggtgcc ggagcgcggg cggggccggg agcgctggtg
    10501 gcgggccgcc gtgcgggaca tccgcttccc gccgcgcagc cggatggacg aggaggtgcg
    10561 ggcggcgttc gacgagctcg gccggctgaa catggcagac gacgtggcgg cgttcgcgcg
    10621 gttccagcag caacgcgatt ccataggcga gtggggcgac gccctgctgt tcgcccgcgg
    10681 cgcgctgaac ctcacggtgc cggagctgcg ccggttctgg gaggactaca tggcgcttta
    10741 cgagcgccac gcccgagccg ctgcgtcacc cacccagggg gctcgccgcg tcctggtgcg
    10801 gttcgtggct ttcccggagg tatgagcggt ccccggtgac ccagcgtgct cttcgcaggg
    10861 cccgcgggcc ctgcgaagag cacgctgggt caccggggac cgctcatacc tccgggaaag
    10921 ccacgaaccg cacca
//
LOCUS       DASRSC010000354         5244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1624738,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000354 DASRSC010000000
VERSION     DASRSC010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1624738"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1479
                     /locus_tag="VFE39_17090"
     CDS             184..1479
                     /locus_tag="VFE39_17090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HET6260355.1"
                     /translation="MDEPSPPTSGGWDLWRLPARAVALVLGVEVAAVAVVIAGISVPT
                     PGAVGLAAFLTLLSVVHTELATGIERIRRRAAETSYFDLSSVWTFAAALLLPPVLATA
                     VIAAVYLHLWQRVWRPAKVPLHRHVYTTATVVLAAYAANAVVQGAGGLPTAAVDVAGV
                     AGIGLAVLLYVLVNTVLVAAVIACTSERVGVRELLGRWDDNALEVATVCMGGLAAVAL
                     GFTPGLIALVLPPILVLHRAVLVHQLEEVACTDAKTGLLNAAAWQVQGARTVRQVHRS
                     QGTAAVLILDLDHFKLVNDSHGHLVGDDVLAAVAAELRATVREQDVVGRFGGEEFVVL
                     LPDLPPGPSGDTEMRAVAERIRKRVACLTVPVGTPDGVLTVRGLSVSVGGAAVPTDGT
                     SLEQVLNAADASLYAAKREGRNLVRFAGPVEVPSPRSAT"
     gene            1526..3526
                     /locus_tag="VFE39_17095"
     CDS             1526..3526
                     /locus_tag="VFE39_17095"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HET6260356.1"
                     /translation="MTALRSVRGPADLKRLEVDQLAPLAAEIRRELVASVSRTGGHLG
                     PNLGVVELTIALHRVFDSPHDTLIWDTGHQAYVHKMLTGRQAGWDQLKKTGGLSGYPC
                     RAESEHDHVENSHASTSLSWADGIARAYALTGQQRHVVAVIGDGALTGGMAWEALNNI
                     AAGERSPGADGAAGRRLGADGAAGDRNIVIVVNDNGRSYAPTIGGLADRLATLRLQPG
                     YERVLDAGKQALARTPVVGGALYAGLHALKAGVKDALSPQELFSDLGLKYFGPVDGHD
                     IAAMESALRRARHFGGPVIVHAVTRKGAGYPPAENDEAEQMHSPAAFDPETGVPTGPP
                     SVGWTSVFSEEMVALGARRPDVVAITAAMLGPTGLAPFAAAFPERCIDVGIAEQHALT
                     SAAGLAAGGLHPVVALYSTFLNRAFDQLLMDVALHRKAVTLVLDRAGVTGNDGPSHNG
                     MWDLSILGIVPGMRVAAPRDAETLREELAEAVAIDDGPTAIRFPKGSVIESVPAVRRL
                     DGVDVLREPGPDGDRDRAVLMVCAGAFGELGIAAAQRLEQQGVTVTVVDPRWVLPVPE
                     MLVRLAREHRLVVTVSDCGRHGGFGSALADVLRAAECDVPLRDLAIPQRFLDHGSRAD
                     VLAAAGLTAQDVARRVTEWAAALPGVQVVDEPTPSAPGEQAR"
     gene            3526..4191
                     /locus_tag="VFE39_17100"
     CDS             3526..4191
                     /locus_tag="VFE39_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6260357.1"
                     /translation="MRVLIVEDERALADAVARGLRREGMAVDVAYDGDTGHEKSVITR
                     YDVVVLDRDLPGMSGDRLCAEIVASGATTRVLMLTASGTLADKVDGLSRGADDYLAKP
                     FDFPELVARCRALGRRATPAVPPLLTAGDLALDPARRTVHRAERPVELTRKEFGVLEV
                     LLGAGGAVVSSEELLERVWDENADPFTTTVRVTVMTLRKKLGDPGVIETVVGAGYRVP
                     AAG"
     gene            4205..>5244
                     /locus_tag="VFE39_17105"
     CDS             4205..>5244
                     /locus_tag="VFE39_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6260358.1"
                     /translation="MAEPGRPVARPAGGSRLGRRGVGLRPRLTLVSTAVVALVSALLL
                     WLGWLLVGGVARAVPSLPPGTTVRVGDMTVPAEQLHDAVGAAARADVLRAGLIAFPLV
                     VAAAAVVSWVLVGRVLGPLYAVTATARRLTVESLDTRTGLHGARGEVAELAAGFDAML
                     DRLQAAFDAQRRFVANASHELRTPLSVLRTEVDVTLSDPAADVEELRRMAEVVRDASR
                     RADDLVAGLLLLARTEGAGLADVRPVDLAVLVVPALAAVRADATRRGLRVQVRAAPAP
                     ATGDPVLLERVAGNLLENAVRHNVVGGWVDVCTGVRDGYAELQVSSTGPVIPADRVQE
                     LFEPFRRGPVART"
BASE COUNT          648 a         1907 c         1922 g          767 t
ORIGIN      
        1 ttcccgcgca cgaccccatc accacgtccc cctggcacta tggaaccgcg cgctcactga
       61 gcgtgacgaa cgagtcgccg agatccaccc cgaagagcga tcacggtcct cctgacacgc
      121 catccacggc tcactagcct cggggacgac acgtacccgt gtcggcggaa ggagatcccg
      181 gtcgtggacg agccgtcgcc gccgacttca ggcgggtggg acctgtggag gctgccggcc
      241 cgggccgtcg ccctcgtcct cggcgtggaa gttgccgccg tcgcggtcgt catcgccggc
      301 atcagcgtcc cgacaccggg ggctgtcggg ctcgcggcgt tcctgacgct gctcagcgtc
      361 gtgcacacgg agctcgccac cgggatcgag cggatccgac gccgcgccgc ggagacctcc
      421 tacttcgacc tgagctccgt gtggaccttc gccgcggcgc tcctgctccc gccggtgctc
      481 gcgacggccg tcatcgccgc ggtgtacctg cacctgtggc agcgggtgtg gcgcccggcg
      541 aaggtgccgc tgcaccggca tgtgtacacg acggccacgg tcgtgctcgc ggcgtacgcc
      601 gcgaacgccg tcgtccaagg ggccggcggc ctgcccaccg ctgccgtcga cgtggccggc
      661 gtagcgggta tcgggctggc tgtgctgctc tacgtcctgg tcaacaccgt gctcgtcgcc
      721 gctgtgatcg cctgtacgag cgagcgggtc ggggtgcgcg agctcctcgg ccggtgggac
      781 gacaacgccc tcgaggtcgc caccgtctgc atgggcgggc tggctgcggt cgcgctcggc
      841 ttcacaccgg gcctcatcgc gctcgtgctg ccaccgatcc tcgtgctgca ccgcgccgtg
      901 ctggtccatc agctcgagga ggtggcgtgc accgatgcga agaccgggct gctgaacgcc
      961 gcggcctggc aggtgcaggg ggcgcggacc gtgcgccagg tacaccggtc ccagggcacc
     1021 gccgccgtcc tgatcctcga tctcgaccac ttcaagctgg tcaacgactc gcacggccac
     1081 ctcgtcgggg acgacgtgct cgcggctgtg gcagcggagc tacgcgccac ggtgcgtgaa
     1141 caggacgtcg tcggccggtt cggtggcgag gagttcgtcg tgctgctacc cgatcttcct
     1201 cccggacctt cgggagacac cgagatgcgc gcggtggccg agcgcatccg caagcgcgtc
     1261 gcgtgtctca ccgttcccgt cggtacgccc gacggcgtcc tcaccgtcag aggcctgtcc
     1321 gtctcggtcg gcggggccgc ggtcccgacc gacggcacga gcctcgagca ggtgctgaac
     1381 gccgccgacg cgtccctcta cgcggcgaaa cgcgaggggc gcaacctcgt ccgcttcgcg
     1441 ggtcctgtcg aggtccccag cccgcgcagt gccacctgaa cggcatcgcc gggccgggct
     1501 caatggtctc tcgtaagctg agccggtgac cgcgttgcgt tccgtccgtg gcccggccga
     1561 tctcaagcgg ctggaggtcg accagcttgc cccgctcgcc gcggagatcc gccgcgagct
     1621 cgtggcttcg gtttcccgca ccggcgggca ccttggcccc aacctcggcg tcgtcgagct
     1681 caccatcgcg ctgcaccggg tgttcgactc cccgcatgac accctcatct gggacaccgg
     1741 ccaccaggcc tatgtccaca agatgctcac cggccggcag gccggctggg atcagctcaa
     1801 gaagaccggt gggctgtccg gctacccgtg tcgggcggag agcgagcacg accacgtcga
     1861 gaacagccac gcgtccacct cgctgtcctg ggccgacggc atcgcccgcg cctacgcgct
     1921 caccggccag cagcgtcacg tcgtcgcggt gatcggcgac ggcgcgctca cgggcggaat
     1981 ggcctgggag gcgctgaaca acatcgcagc cggcgagcgg agccctggcg ccgacggcgc
     2041 cgcaggccgc aggctcggcg ccgacggcgc tgcgggggac cgcaacatcg tcatcgtcgt
     2101 gaacgacaac ggccggtcgt acgcgccgac gatcggcggg ctggccgacc ggctggccac
     2161 gctccgcctg cagccggggt acgaacgggt cctcgacgcg ggcaagcagg ccctcgcgcg
     2221 cacccctgtc gtcggcggtg cgctctacgc cgggctgcac gccctcaagg cgggtgtgaa
     2281 ggacgcgttg agcccgcagg agctgttctc cgacctcggc ctgaagtact tcgggcccgt
     2341 cgacgggcac gacatcgccg cgatggagtc agcgctgcgc cgggcccgcc atttcggcgg
     2401 cccggtgatc gttcatgccg tcacgcgcaa gggcgcgggc tacccgcccg cagagaacga
     2461 cgaggccgag cagatgcaca gcccggccgc gttcgaccca gagacagggg tccccaccgg
     2521 gccgccttcg gtcggctgga ccagcgtgtt ctccgaggag atggtggcgc tcggggccag
     2581 gcggcccgac gtcgtcgcga tcacggcggc gatgctcggc cccaccgggc tcgcgccgtt
     2641 cgccgcggcg ttccccgagc gctgcatcga cgtcggcatc gccgagcagc acgcgctcac
     2701 ctccgccgcg gggctggccg cgggcgggct gcacccggtc gtcgcgctgt actcgacatt
     2761 cctcaaccgg gccttcgacc agctgctgat ggacgtggcg ctgcaccgta aggccgtcac
     2821 cctcgtgctg gaccgggccg gcgtcaccgg caacgacggg ccgtcccaca acgggatgtg
     2881 ggacctgtcg atcctcggga tcgtgccggg catgcgggtc gccgcgccgc gcgacgccga
     2941 gaccctgcgg gaggagctgg ccgaggcggt cgcgatcgac gacggtccca ccgcgatccg
     3001 gttccccaag ggctcggtga tcgagtccgt gcccgcggtg cggcgcctcg acggtgtgga
     3061 cgtcctgcgc gagcccggcc cggacggcga ccgggatcgc gcggtgctca tggtctgtgc
     3121 cggtgcgttc ggtgagctcg gcatcgcggc ggcgcagcgg ctggagcagc agggcgtcac
     3181 ggtcaccgtc gtcgacccgc gttgggtgct gccggtgccc gagatgctcg tgcggctggc
     3241 gcgcgagcac cggctcgtcg tcactgtctc cgactgcggc cgccacggcg ggttcggttc
     3301 cgccctcgcc gacgtactgc gcgccgccga gtgcgatgtc ccgctgcgcg acctggcgat
     3361 cccgcagcgc ttcctcgacc acggcagccg cgccgacgtg ctcgccgccg ccgggctgac
     3421 cgcacaggac gtggcgcggc gggtcaccga gtgggccgct gccctgccgg gggtccaggt
     3481 cgtcgacgag cccacgccgt cggccccggg agaacaggcg cgctgatgcg cgtgctcatc
     3541 gtcgaggacg agcgtgcgct ggcggacgcg gtcgcacggg ggctgcgccg cgagggtatg
     3601 gccgtcgacg tggcctacga cggcgacacc ggccacgaga agtccgtcat cacccgctat
     3661 gacgtggtgg tgctcgaccg ggatctgccc ggcatgtcgg gtgaccggct gtgtgcggag
     3721 atcgtcgcgt ccggggccac cacgcgggtg ctgatgctca ccgccagcgg cacgctcgcc
     3781 gacaaggtcg acggtctgtc ccgcggcgcg gacgactacc tcgccaagcc cttcgacttc
     3841 cccgagctcg tggcgcgctg ccgggcgctc gggcgccggg ccacccccgc cgtgccgccg
     3901 ctgctcacgg cgggcgacct cgcgcttgac ccggcgcgcc gcaccgtgca ccgggcggag
     3961 cggcccgtgg agctgacgcg caaggagttc ggcgtgctgg aggtcctgct cggtgccggt
     4021 ggggccgtgg tgagcagtga ggagctgctg gagcgcgtct gggacgagaa cgccgacccg
     4081 ttcaccacca ccgtccgggt caccgtgatg accctgcgca agaagctcgg tgaccccggt
     4141 gtgatcgaga ccgtggtcgg tgccgggtac cgggtaccgg ccgcgggctg agcggggtgc
     4201 tgccatggcc gaaccagggc ggcccgtggc ccggccggcc ggcgggtcgc gactcggtag
     4261 gcgaggggtg gggctgcgcc ctcgactgac gctggtcagt acggccgtcg tcgcgctggt
     4321 gagcgctctg ctgctctggc tgggctggct gctggtcggc ggcgtggccc gtgcggttcc
     4381 ctcgctgccg cccggtacga ccgtccgtgt gggtgacatg accgttcccg ccgaacagct
     4441 ccacgatgcc gtcggcgccg ccgcgcgggc cgacgtgctg cgggcaggac tgatcgcgtt
     4501 cccgctcgtc gtagccgcgg ctgcggtggt gtcctgggtg ctggtcggcc gggtgctcgg
     4561 tccgctgtac gcggtgacgg ccaccgcccg acggttgacc gtcgagtcgc tggacacccg
     4621 cacggggctg cacggcgcgc gcggcgaggt cgccgagctc gccgcgggat tcgacgcgat
     4681 gctggaccgg ctgcaggccg cgttcgacgc gcagcgccgg ttcgtcgcca acgccagcca
     4741 cgaactgcgt acgccgctct cggtgttgcg cacggaggtg gacgtcaccc tgtccgatcc
     4801 cgcggccgat gtggaggagc tacggcggat ggccgaggtc gtccgggacg ccagccgccg
     4861 cgctgacgac ctcgtcgccg ggttgctgct gctggcccgc accgaaggtg ctgggctggc
     4921 cgacgtgcgg cccgtcgacc tcgccgtgct cgtcgttccg gcactggccg ccgtccgcgc
     4981 cgacgccacg cggcgagggc tgcgggtaca ggtccgggcg gcccccgcac ccgcaaccgg
     5041 cgacccggtg ctgctcgagc gcgtggcggg gaacctgctg gagaacgcgg tgcgacataa
     5101 cgtcgtcggc ggctgggtgg acgtctgcac cggcgtgcga gacggctacg cggaacttca
     5161 ggtgtcctcc accggccccg tgatccccgc ggaccgggtg caggagctgt tcgagccgtt
     5221 ccgtcgcggg cccgtggcgc gcac
//
LOCUS       DASRSC010000355         5210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_481763,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000355 DASRSC010000000
VERSION     DASRSC010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5210
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481763"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..989
                     /gene="era"
                     /locus_tag="VFE39_17110"
     CDS             57..989
                     /gene="era"
                     /locus_tag="VFE39_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015800096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET6260359.1"
                     /translation="MTGGFAEVPDGFRSGFACFVGRPNAGKSTLTNALIGSKIAITSS
                     RPQTTRHAIRGVVHRPDAQLVVVDTPGLHKPRTLLGSRLNDVVRETWAEVDVIGFCVP
                     ADAPVGRGDEFIVNELRSVASRTPVLGIVTKTDLAQPEQIVERLTQLNGLMDFAEVVP
                     CSAVDGFQVDLLTDLLVARLPEGPPLYPDGELTDEPEETLVAELIREAALEGVRDEMP
                     HSIAVVIEEMVPREGRHEKAAPMLNVHANIYVERQSQKGIMIGKGGERLRQVGTDSRR
                     QIEALLGTKVFLDLHVKIAKDWQRDPRQLRKLGF"
     gene            1426..2199
                     /gene="recO"
                     /locus_tag="VFE39_17115"
     CDS             1426..2199
                     /gene="recO"
                     /locus_tag="VFE39_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010695659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET6260360.1"
                     /translation="MALYRDTGVVLRVQKLGEADRIITLLTRRRGKVRAVAKGVRRTK
                     SRWGARLEPFNHVDVQCYTGRSLDIVTQAETVDAFGVGIIGDYPRYTAGCAILETADR
                     LVSEEGEPAMRVYLLLVGAIRALSGRERDPSLVLDAFLLRAMTHAGWAPAISDCARCA
                     EPGPHPAFNIAAGGAVCPRCRPAGSVMPSRETFALLDALLHGDWDIADAAAPTTRRDT
                     SGLVAAHLQWHLERQLRSLPLVERRSFPRAERASVQETI"
     gene            complement(2223..2474)
                     /locus_tag="VFE39_17120"
                     /pseudo
     CDS             complement(2223..2474)
                     /locus_tag="VFE39_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005152028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(2404..2973)
                     /locus_tag="VFE39_17125"
     CDS             complement(2404..2973)
                     /locus_tag="VFE39_17125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260361.1"
                     /translation="MLLLLPAAVALLVPAARPAVAGAGRGLAGVERTRVTTVLGGVDP
                     TAAEPSAPRALRYLAARLPVGLLGGVVLTLLVLGTGFAATILWSWITGTPWLLEDEQA
                     TVFSGEILAYYTVPGMVLLYLGLAGATGVVRWERALLARLLSPSREEQLARRVTELAG
                     TRDAVVAGGGAATARPRQPRRPPRPPHPA"
     gene            complement(3056..3820)
                     /locus_tag="VFE39_17130"
     CDS             complement(3056..3820)
                     /locus_tag="VFE39_17130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HET6260362.1"
                     /translation="MISSPPPDGIVGWVVALMDALGAPGVGLAIALENVFPPLPSEVF
                     LPLAGFAAARGETSLVAAIVWTTLGSLVGALVLYGIGAALGRDRLRAIVDRMPLVNLR
                     DVDRAEEWFARHGAKAVFLGRMVPLVRSLISVPAGVERMHPALFAALTTAGSLIWNTA
                     FVLAGYTLGENWHVVESYAGIASKVVLVAVVLAVVVFVAKRLSRRGQQRGGDGVPLPS
                     DDNSPTEQLPAVLEAAPTERIPAVPPGPPMRHGPHQ"
     gene            3917..4702
                     /locus_tag="VFE39_17135"
     CDS             3917..4702
                     /locus_tag="VFE39_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET6260363.1"
                     /translation="MARFQPPPPHPSGARPPAIPRELVPNHVALVMDGNGRWANARGL
                     PRIEGHRRGEASLMDVARGAIDIGVRWLSAYAFSTENWKRSPDEVRFLMGFNRDVIHR
                     RVDEMHEMGVRVRWAGRRPRLWRSVVKELEIAEEKTRHNDVLTLTMCVNYGGRAEIAD
                     SVRQIARLVAEGRIKPDKIDERTIARYLDEPDMPDVDLFVRSSGEQRTSNFLLWQSAY
                     AEMVFLNTLFPDFDRRHLWEAIEIYARRDRRFGGALDKPGTAS"
     gene            4699..5103
                     /locus_tag="VFE39_17140"
     CDS             4699..5103
                     /locus_tag="VFE39_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260364.1"
                     /translation="MSSNAIPDMLAVARAALERFHDVTVDDDGALTFAHGGVVCALQG
                     LELEEGLPVLNLTCVVAWDLPPESDIPTRVALGAGEGLFGTPRVIRGQAGWDVTLRYA
                     FPAVGLDDKAMGTLLMLVVSSASSMRAELTES"
BASE COUNT          770 a         1859 c         1896 g          685 t
ORIGIN      
        1 cgcggatacg agtgccagag cacccgtttc cgcgcacgac cggcgggggg tgcggcgtga
       61 ccggcgggtt cgccgaggtc ccggacggct tccggtccgg gttcgcctgc ttcgtcgggc
      121 ggcccaacgc gggcaagtcg acgctgacca acgcactcat cggatcgaag atcgcgatca
      181 cctccagcag gccgcagacc acgcgccatg ccatccgcgg ggtcgtgcac cggcccgacg
      241 cgcagctcgt ggtggtcgac accccgggcc tgcacaagcc gcgcacgttg ctcggtagca
      301 ggctcaacga cgtcgtccgc gagacctggg ccgaggtcga cgtgatcggc ttctgcgtgc
      361 cggccgacgc gcccgtgggc aggggcgacg agttcatcgt caacgaactg cgctcggtcg
      421 ccagccgcac accggtgctc gggatcgtca ccaagaccga cctcgcgcag cccgaacaga
      481 tcgtcgaacg gctcactcag ctcaacgggc tcatggactt cgccgaggtg gtgccctgct
      541 cggcggtcga cgggttccag gtcgacctgc tcaccgacct gctcgtcgcc cggctgccgg
      601 agggcccccc gctctacccc gacggcgagc tcaccgacga gccggaggag acgctggtcg
      661 cggagctgat ccgcgaggcc gcgctggagg gcgtgcgcga cgagatgccg cactccatcg
      721 ccgtggtgat cgaggagatg gtgccgcgcg agggcaggca cgagaaggct gcgccgatgc
      781 tgaacgtgca cgccaacatc tacgtggagc ggcagagcca gaagggcatc atgatcggca
      841 agggcggcga gcggctacgc caggtcggca ccgactcgcg gcggcagatt gaggcgctgc
      901 tcggcacgaa ggtgttcctc gacctgcacg tcaagatcgc gaaggactgg cagcgcgacc
      961 cccgccagct gcgcaagctc ggcttctgag ggccgacgat cgccggcccg cgaggccggc
     1021 cctacctgcc ggcagcggag aacgttgccg ggatcctgct cggaggagcg cgggacgcca
     1081 ccgttccccg gctcgttcta cggcctcgac gcggagcagg ccgaggtgac cctgtggcgt
     1141 cagatcgacg gcgcgggcga cgcgggggaa cggggggccc gacatcgacg gccggcatgc
     1201 cgcctggctg cgcaccgtca ccgactggaa cccggtcagc gcggtcaccg cggccgtccg
     1261 cgagctctgc ggcacccgac ggcgccgacg aacgggacat ggccgctgga agcacccggt
     1321 gctcgcctcg ctgggctgga cggcgcttcc tgctgctggt gttcgtgcca ttgtgcacgg
     1381 cccggtacgc ccggccgcgg ggctgacacg ccgtacgctg accgcgtggc tctctaccgc
     1441 gacaccggcg tggtgctgcg ggtgcagaag ctcggcgaag ccgaccggat catcacgctg
     1501 ctcacccgcc gccgcgggaa ggtgcgcgcg gtggcgaagg gggtgcggcg cacgaagtcg
     1561 cggtggggtg cgcggctcga gccgttcaac cacgtcgacg tgcagtgcta caccggccgc
     1621 tcgctcgaca tcgtcaccca ggccgagacc gtcgacgcgt tcggcgtggg catcatcggc
     1681 gactaccccc gctacacggc aggttgcgcg atcctcgaga cggccgaccg gctcgtctcg
     1741 gaggagggcg agcccgcgat gcgggtgtac ctgctgctgg tgggggcgat ccgcgccctg
     1801 tccggccggg agcgggaccc atcactcgtg ctggacgcgt tcctgctgcg tgcgatgacg
     1861 cacgccggct gggctcccgc gatcagcgac tgcgcgcgct gcgccgagcc cggcccccac
     1921 ccggcgttca acatcgccgc cgggggagcg gtgtgcccgc gctgccgccc ggccggctcg
     1981 gtcatgccgt cgcgggagac gttcgcgctg ctggacgccc tgctgcacgg cgactgggac
     2041 atcgccgacg ccgcggcccc gaccacccgc cgcgacacca gcgggctcgt ggccgcgcac
     2101 ctgcagtggc atctggagcg gcagctgcgc tcgttgccgc tggtggagcg gcgctcgttc
     2161 ccgagggccg agcgtgcatc ggttcaggag acgatctgaa ccagtgcgtg atcgcgcctg
     2221 tctcaggagc cgaggtagcg caggatcgcc agcacccgcc tgctgtaccc ggccgcctcg
     2281 gtgatcccga gcttcccaaa gatcgacgcg acgtgcttct ccaccgcgct caccgacatc
     2341 cgcaaccgct ccgccgtgcc cgcgttcgtc agcccctcgg ccatggcgtc gagcacgtcg
     2401 cgctcacgcg gggtgaggcg ggcgaggggg tcgacgcggc tgccgcgggc gagcagttgc
     2461 cgcaccacct ccggcgacga cggcgtcgcg ggtgcccgcc agctccgtga cgcggcgggc
     2521 gagctgctcc tcccgcgacg gcgagagcag ccgggccagc agcgcgcgct cccaccggac
     2581 gacgccggtc gctcctgcca gaccgaggta gagcagcacc atcccgggca ccgtgtagta
     2641 ggcgaggatc tcgccactga agacggtggc ctgctcgtcc tcgagcagcc acggcgtccc
     2701 ggtgatccag gaccacagga tggtggccgc gaaccccgtg ccgaggacga gcaaggtgag
     2761 cacgaccccg ccgaggagcc ctaccggcag ccgggcggcc aggtagcgca gggcccgcgg
     2821 ggcggagggt tcggcggcgg tcgggtcgac cccgcccaaa accgtcgtca ccctggtccg
     2881 ctcgacgccg gccagcccac ggccagcacc cgccaccgcc ggccgggcag cggggacgag
     2941 cagggccacg gcggccggca gcaggagaag cacgtgcgcg gcggccgtga gggcgccgag
     3001 ggcgagccct gtgcccctcc gggcagcccg cccgatcgtc cctaccagcg ggaggctact
     3061 ggtggggccc gtgccgcatc ggcgggccgg gggggaccgc cgggatccgt tccgtgggcg
     3121 cggcctcgag gaccgctggg agctgctccg tggggctgtt gtcatccgac gggagaggaa
     3181 caccatcgcc gccgcgctgc tgcccccgcc gcgacagccg cttcgcgacg aacacgacga
     3241 cggccagcac gaccgccacg agcaccacct tcgaggcgat ccccgcgtag ctctcgacga
     3301 cgtgccagtt ctcgccgagc gtgtatccgg ccagcacgaa cgcggtgttc cagatcaagc
     3361 tcccggctgt ggtgagtgcg gcgaacagcg cggggtgcat gcgctcgacg ccagcgggca
     3421 ccgagatgag gctgcgcacc agcggcacca tccggccgag gaacacggcc ttggcgccgt
     3481 gccgggcgaa ccactcctcg gcgcggtcga cgtcgcgcag gttcacgagc ggcatgcggt
     3541 cgacgatcgc gcgtaggcgg tcgcggccga gcgcggcgcc gatgccgtac agcaccagtg
     3601 ccccgacgag cgagccgagc gtggtccaga cgatcgcagc gacgaggctc gtctcgccgc
     3661 gggccgcagc gaatccggcg agtgggagga agacctcgct cggaagtggg gggaagacgt
     3721 tctcgagggc gatggcgagc ccgacgccgg gcgcgccaag ggcgtccatc agggcgacga
     3781 cccagccgac gatcccgtcg ggcggagggg agctgatcat gccaccgatg gtaggaaagt
     3841 ccgatccgtc gggtactggg gtattcgccg gacgcaccct gcggtgcgcc acaccgccgc
     3901 cgtaggctgg cccgccgtgg cccgattcca gcccccgccg ccgcacccgt cgggtgcgcg
     3961 gcccccggcg attccccgtg agctcgtgcc gaaccatgtc gccctcgtca tggacggcaa
     4021 cggtcgctgg gcgaatgccc gtgggctccc ccggatcgag gggcaccggc gcggcgaggc
     4081 gtccctgatg gacgtcgccc gcggagcgat cgacatcggt gtccgctggc tgtcggccta
     4141 cgccttctcc acggagaact ggaagcgcag ccccgacgag gtgcgcttcc tcatgggctt
     4201 caaccgtgac gtcatccacc gtcgcgtcga cgagatgcac gagatgggtg tgcgcgtccg
     4261 atgggcgggc cgccggcccc ggctgtggcg ttcggtggtc aaggagctgg agatcgccga
     4321 ggagaagacg aggcacaacg acgtcctgac cctcaccatg tgcgtcaact acggcgggcg
     4381 cgccgagatc gccgactccg tgcggcagat cgcccggctc gtcgcggagg ggcggatcaa
     4441 gcccgacaag atcgacgagc ggacgatcgc gcggtacctc gacgagccgg atatgcccga
     4501 cgtcgacctg ttcgtgcgct cctcgggtga gcagcgcacg tcgaacttcc tgctctggca
     4561 gtcggcctac gccgagatgg tcttcctcaa cacgctgttc cctgacttcg accggcgcca
     4621 cctgtgggag gccatcgaga tctacgcgcg ccgcgaccgc cgcttcggcg gggcgctgga
     4681 caagccgggc accgcgtcat gagcagcaat gcgatcccgg acatgctcgc cgtcgcccgc
     4741 gcggcgctgg agcgtttcca cgacgtcacc gttgacgacg acggtgcgct cacgttcgcc
     4801 cacggcggcg tcgtgtgcgc gctgcagggc ctcgagctgg aggagggact gcctgtcctc
     4861 aacctcacct gcgtcgtggc ctgggacctg cccccggagt ccgacatccc cacccgggtg
     4921 gcgctcggcg ccggggaggg cctgttcggc acgccgcgcg tgatccgcgg gcaggccggg
     4981 tgggacgtca cactgcgcta cgcgttcccc gccgtcgggc tcgacgacaa ggccatgggc
     5041 acgctgttga tgctggtcgt gagcagcgcg tcgagcatgc gggcggaact gaccgaaagc
     5101 taggagatca ggaggacagg tgcacgagcg gtagccgcgc cagctcgaca ggtgtgagag
     5161 tcccggccgc tgcgagcacc cgggactgag cctcctgcat ccgggcgtcg
//
LOCUS       DASRSC010000356         5143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2281402,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000356 DASRSC010000000
VERSION     DASRSC010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2281402"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..737
                     /locus_tag="VFE39_17145"
     CDS             <1..737
                     /locus_tag="VFE39_17145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020635854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HET6260365.1"
                     /translation="LLARADERGLQPRREEVDLDEVAESERGRPADSAVVAEVHAQPV
                     RVIGDRGQLARVVRNLVDNARRHARSRVLVMVTREEADTAVIEVSDDGPGVPVADRGR
                     VFERFVRLDDARARSDGGSGLGLAIVAEVVTAHGGTVEVGDAPGGGALFRVRLPAQPY
                     GPVADLDPPVATTVRPVPHPRARPVAPVAQPVRDQDAKRDPETSPWGLPALEPTSDPR
                     GLPAQLPRQWSIRSGGAADQPGSIIL"
     gene            complement(713..1387)
                     /locus_tag="VFE39_17150"
     CDS             complement(713..1387)
                     /locus_tag="VFE39_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017982770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6260366.1"
                     /translation="MRVLLVEDEVPLAEMVRRGLAREGHSVQVVHNGLAGLAAASTRQ
                     HDVVVLDIMLPGLSGYRVLQRLREAEIWTPVLMLTAKDGEYDEADAFDLGADDYLVKP
                     FSFVVLLARLRALLRRSGDARPAVLTVGSLRLDPARHRVHRGDTEITVTPREFGVLEH
                     LMRHAGEVVTKTDILRAVWDAHYEGEVNVVEVYVGYLRRKIDAPFGTSTIETVRGVGY
                     RMIEPG"
     gene            1509..3110
                     /locus_tag="VFE39_17155"
     CDS             1509..3110
                     /locus_tag="VFE39_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010227159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HET6260367.1"
                     /translation="MPVATSGPVLRSAVDPSGQDAARHAASHRDLVADLRQKLAAARL
                     GGPERARMRHVERGKMLPRERVDSLVDPSSPFLELSPLAAGGMYDDEAPAAGLITGVG
                     RVAGRECVIVANDATVKGGTYYPMTVKKHLRAQEVALHNRLPCIYLVDSGGAYLPEQD
                     SVFPDREHFGRIFYNQATMSGLGIPQIAAVLGSCTAGGAYVPAMSDEAVIVRNQGTIF
                     LGGPPLVQAATGEVVTAEELGGGDLHSRISGVTDHLANDDAHALRIVRSIVGTLGPRA
                     PRPWDVLPAEEPAVDPASLYDVVPTDSRTPYDVREVIARIVDGSRFHEFKAEYGTTLV
                     TGFARLHGHPVGIVANNGILFSESALKGAHFIELCDQRGVPLVFLQNISGFMVGREYE
                     AGGIAKNGAKMVTAVASTRVPKLTVIIGGSFGAGNYSMCGRAYSPRFLFMWPGARISV
                     MGGEQAAMVLSAVRGNGNGESDEDFRDRIRAQYEHQGHPYYSTARIWDDGVIDPKDTR
                     TVLGLALSVAANAPLADPAFGVFRM"
     gene            3255..>5143
                     /locus_tag="VFE39_17160"
     CDS             3255..>5143
                     /locus_tag="VFE39_17160"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET6260368.1"
                     /translation="MFDVVLVANRGEIAVRVLRTLRQLGVRSVAVYSDADADAPHVAL
                     ADVAVRIGPAPAAQSYLSIPAVIDAARATGAQAIHPGYGFLSENTAFAAACEEAGIVF
                     VGPPAAAIEAMGDKIRAKQTVAKAGVPVVPGSDGAGLSDDDLVAAVEQIGYPVLLKPS
                     AGGGGKGMREVHRPDQLADAIASARREARGSFGDDTLLVERLVSTPRHIEIQVLADAH
                     GNVVHLGERECSLQRRHQKIVEEAPSALLTEAQRAAMGAAAVEAARAVGYTGAGTVEF
                     IVEGDGAEGDLPFFFMEMNTRLQVEHPVTELVTGLDLVELQLRVAAGEPLPITQEDVR
                     LTGHAVEVRVYAEDPAAGFLPTGGRILALREPAGPGIRVDSGVAEGLVVGSDYDPMLA
                     KVIAHGADRAEAVRRLDAALRDTMLLGLYTNIGFLRALLADDDVRSARLDTGLVGRRG
                     DAWTAAELPADVLVAAAAHAMLELEPTGPVVDPFDVPGGWRIGEPAWTTWQMLVAGHE
                     PVIVRVSGRASSAECVVGDRTPVSVRAHRRPDAIEVTVDGVTRRYHWAHDGETLWLGR
                     DGHAWGVREQAPLEAAATAEAGAGGPVLSPMPGTVTVVEVAEGQQVAAGDRLVVVEAM
                     KMEHVL"
BASE COUNT          698 a         1843 c         1873 g          729 t
ORIGIN      
        1 tgctgctggc cagggccgac gagcgcggcc tgcagccacg ccgcgaggag gtggacctcg
       61 acgaggtcgc cgagagcgag cgcggccgcc ccgccgacag tgcggtggtg gccgaggtgc
      121 acgcccagcc ggtgcgggtg atcggcgacc gcggacagct ggcgagggta gtgcgcaacc
      181 tcgtcgacaa cgcgcgccgg catgctcgtt cgcgggtgct cgtcatggtc acgcgggagg
      241 aggccgacac cgcggtgatc gaggtgtcgg acgacgggcc gggggtgccg gtcgccgacc
      301 ggggcagggt gttcgagcgg ttcgtgcggc tcgacgacgc ccgcgcccgg tccgacgggg
      361 gttccgggct gggcctggcg atcgtcgccg aggtcgtgac cgcgcacggc ggcaccgtgg
      421 aggtgggcga cgctccgggc ggcggagcgc tgttccgggt gcggctgccg gcgcagccct
      481 acgggccggt cgccgacctc gatccgcctg tcgcgacgac ggtccggccg gtgccgcacc
      541 cgagggcgag accggtcgcg cctgttgcgc agccggtgcg tgaccaggac gcgaagcgcg
      601 atccggaaac ttcgccgtgg ggcctcccgg ccctcgaacc cacgagcgat ccgcgcgggc
      661 tgccggcgca gctgccccgg cagtggtcca tccggtcggg cggggcggcc gatcagcccg
      721 gctcgatcat cctgtagccc accccgcgca ccgtctcgat ggtgctcgtg ccgaagggtg
      781 cgtcgatctt gcggcgcagg tagccgacgt acacctcgac gacgttgacc tcgccctcgt
      841 agtgggcgtc ccagaccgcc cgcaggatgt cggtcttcgt gacgacctcg cctgcatgcc
      901 gcatcaggtg ctccagcacg ccgaactctc gcggggtcac cgtgatctcg gtgtcgcccc
      961 ggtgcacgcg gtgccgggcc ggatcgaggc gcagcgagcc gacggtcagg acggcaggcc
     1021 tggcgtcccc cgagcgccgc aggagggcgc gcagccgggc gaggagtacg acgaacgaga
     1081 acggcttcac gaggtagtcg tcggcgccga ggtcgaacgc gtcggcctcg tcgtactcgc
     1141 cgtccttcgc ggtcagcatg agcaccgggg tccagatctc ggcctcgcgc agccgttgca
     1201 gcacgcggta gccggagagg cccggcagca tgatgtcgag caccacgacg tcgtgctgcc
     1261 gtgtgctcgc cgcggccagg cctgccagtc cgttgtgtac gacctgtacc gagtgtccct
     1321 ctctcgccag tccgcgccgc accatctcgg ccagcggtac ctcgtcctcg accagcagta
     1381 ctcgcacggg gtcagaccgt agcgggggcc gccgaacgtc ccgggcccag ctccaccgca
     1441 cccaccggac ggtggcgccg caagttaatg agcgttaaca tcggctgcag ctcaccgagg
     1501 gaggtcgtat gcccgtcgcc acgtccggtc cggtgctccg cagcgccgtc gacccgtccg
     1561 gccaggacgc cgcgcgccac gccgcctcgc accgcgacct ggtcgccgac ctgcggcaga
     1621 agctggccgc ggcgcggctc ggtggtccgg agcgggcacg catgcgacac gtcgagcgcg
     1681 gcaagatgct gccgcgtgag cgcgtcgact cgctggtgga cccgtccagc ccgttcctcg
     1741 agctctcgcc gctcgccgcg ggcggcatgt acgacgacga ggcccccgct gccgggctga
     1801 tcaccggcgt cggccgggtg gccgggcgcg aatgcgtcat cgtggcgaac gacgccaccg
     1861 tcaagggcgg cacctactac ccgatgacgg tcaagaagca cctgcgcgct caggaggtgg
     1921 cgctgcacaa ccggctgccg tgcatctacc tcgtcgactc cgggggcgcg tacctgcccg
     1981 agcaggacag cgtcttcccc gaccgcgaac acttcggtcg gatcttctac aaccaggcca
     2041 cgatgtccgg gctcggcatc ccgcagatcg ctgcggtgct cggctcctgc accgcgggcg
     2101 gtgcctacgt gccggcgatg agcgacgagg cggtgatcgt ccgcaaccag ggcacgatct
     2161 tcctgggtgg gccgccgctg gtgcaggcgg caaccggcga ggtggtcacg gctgaggagt
     2221 tgggtggcgg cgacctgcac tcgaggatct ccggggtcac cgaccacctg gcgaacgacg
     2281 acgcgcacgc gctgcggatc gtccggtcga tcgtcggaac gctcggtcca cgggccccgc
     2341 ggccgtggga cgtcctcccc gcggaggagc cggcggtcga cccggcgtcg ctctacgacg
     2401 tggtgcccac cgactcccgc accccctacg acgtgcgcga ggtgatcgcc cggatcgtcg
     2461 acgggtcgcg gttccacgag ttcaaggccg agtacggcac cacgctggtc accggcttcg
     2521 cgcggctgca cgggcacccg gtcggcatcg tcgcgaacaa cgggatcctg ttctccgagt
     2581 cggcgctcaa gggcgcgcat ttcatcgagc tgtgcgacca gcgtggcgtc cctctggtgt
     2641 tcctccagaa catctcgggg ttcatggtcg gccgcgagta cgaggcgggc ggcatcgcga
     2701 agaacggcgc gaagatggtc acggccgtcg cctcgacccg ggtgcccaag ctcaccgtga
     2761 tcatcggcgg ctcgttcggc gccgggaact actcgatgtg cgggcgggcg tactccccga
     2821 ggttcctgtt catgtggccg ggcgcgcgca tctccgtcat gggcggtgag caggccgcga
     2881 tggtgctgtc cgccgtgcgc ggcaacggga acggggagtc cgacgaggac ttcagggacc
     2941 gcatccgcgc ccagtacgag caccagggcc acccctacta ctccacggcg aggatctggg
     3001 acgacggcgt gatcgacccg aaggacacga gaaccgtcct cggcctcgcg ctctcggtgg
     3061 cagcgaacgc gcccctggcc gacccggcat tcggcgtctt ccggatgtga gccccgtgcc
     3121 cgcccgccct gccctggaaa ccccgccgat caaggcgctg acgtcgcttg tcgagctcca
     3181 ctcaccacat ctgcgccttg atcaccgctc gtgggccggt gcggtgccgc tgttccagcc
     3241 ttcgggaggc ttccgtgttt gacgtcgtgc tcgtcgccaa ccggggtgag atcgccgtgc
     3301 gtgtgctgcg cacgctgcgg cagctggggg tgcggtcggt ggccgtgtac tccgatgccg
     3361 atgccgacgc gccgcacgtc gccctcgccg atgtcgccgt ccggatcggc cccgcgcccg
     3421 ccgcgcagag ctacctctcg atcccagccg tgatcgacgc ggcgcgggcc accggagccc
     3481 aggcgatcca tcccgggtac gggttcctga gcgagaacac cgccttcgcc gccgcctgcg
     3541 aggaggcggg catcgtgttc gtcgggccac cggcggccgc gatcgaggcg atgggcgaca
     3601 agatccgcgc caagcagacc gttgccaagg ccggggtccc cgtcgtcccg ggctcggacg
     3661 gcgccggcct gtcggatgac gacctcgtgg ctgccgtcga gcagatcggg tatccggtgc
     3721 tgctcaagcc cagcgcgggc ggtggcggca agggcatgcg cgaggtgcat cgccccgacc
     3781 agctcgcaga cgccatcgcg tccgcccggc gcgaggcccg tggttccttc ggcgacgaca
     3841 cgctgctggt cgagcggctc gtcagcaccc ctcgccacat cgagatccag gtgctggccg
     3901 acgcccacgg caacgtcgtg cacctcggcg agcgcgagtg cagcctgcag cggcggcacc
     3961 agaagatcgt cgaggaggca ccgtcggcgc tgctgaccga ggcgcagcgc gccgcgatgg
     4021 gcgcggcagc cgtcgaggcg gcgcgggcgg tgggttacac cggtgcaggg acagtggagt
     4081 tcatcgtgga gggcgatgga gccgagggcg acctgccgtt cttcttcatg gagatgaaca
     4141 ccaggctgca ggtggaacac ccggtcaccg agctcgtcac cgggctcgac ctcgtcgaac
     4201 tgcagctgcg ggtcgccgcc ggcgagccac ttcccatcac ccaggaggac gtgcgcctca
     4261 ccgggcacgc cgtcgaggtg cgggtctacg ccgaggatcc ggccgcaggc ttcctgccca
     4321 ccggcggccg gatcctggcg ctgcgcgaac cggccgggcc ggggatccgc gtcgactccg
     4381 gagtggccga gggcctcgtc gtcggctcgg actatgaccc gatgctggcc aaggtcatcg
     4441 cccacggtgc cgaccgtgcc gaggccgtac gccgcctcga cgctgcgctg cgcgacacga
     4501 tgctgctcgg cctgtacacc aacatcggct tcctgcgcgc cctgctcgcc gacgacgacg
     4561 tccggtcggc gcggctggac accggtctcg tcggccgtcg cggggacgcc tggaccgccg
     4621 ccgagctgcc cgccgacgtg ctcgtcgcgg cggccgccca cgccatgctg gagctcgaac
     4681 ccaccggtcc ggtggtcgac ccgttcgacg tgcccggcgg ctggcgcatc ggtgagcccg
     4741 cgtggacgac atggcagatg ctcgtggcgg ggcacgagcc cgtgatcgtc cgggtgagcg
     4801 gacgggcctc gtcggccgag tgtgtcgtcg gcgaccgcac tccggtgtcc gtgcgtgcgc
     4861 accgtcgccc cgacgccatc gaggtgaccg tggacggcgt gactcgccga taccactggg
     4921 cgcacgacgg cgagaccctg tggctcgggc gcgacgggca cgcctggggt gtccgcgagc
     4981 aggccccgct cgaggcggcc gccacagcgg aggcgggcgc aggcggaccc gtgctctccc
     5041 cgatgcccgg caccgtcacc gtcgtcgagg tcgccgaggg gcagcaggtc gccgcgggcg
     5101 accgcctcgt cgtcgtcgag gcgatgaaga tggagcacgt gct
//
LOCUS       DASRSC010000357        16133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1626553,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000357 DASRSC010000000
VERSION     DASRSC010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 16133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16133
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1626553"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="VFE39_17165"
     CDS             complement(<1..1545)
                     /locus_tag="VFE39_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020645324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET6260369.1"
                     /translation="MITATDLELRAGSRILLSGATLRVQPGDRIGLVGRNGAGKTTSM
                     RVLAAEGEPYAGSVGANSPVGYLPQDPREGDLTVTAKDRVLSARGLDEMLRKMEKAQT
                     AMAELVDGAQNDKAVREYGRIEERFSALGGYAAESEAARICAHLGLPDRVLHQQISTL
                     SGGQRRRVELARILFAASDGGGGGAPSATTLLLDEPTNHLDADSITWLRSFLAAHDGG
                     LVVISHDTELLAAVVNKVWFLDATRSEVDVYNMDWKRYQEARATDEKRRRRERANAEK
                     KASVLHTQAAKMGAKATKAVAAKNMARRADQMLAGLEPERKNDRVAKIRFPDPSPCGR
                     TPLTAEGLSKAFGSLEVFTGVDLAVDRGAKVVVLGLNGAGKTTLLRLLAGTETPDAGR
                     VLPGHGLRTGYYAQEHDTLDLDATVWENVRHASPDTPEQQLRTLLGSFMFSGDQLEQP
                     AGTLSGGEKTRLALAGLVSSAANVLLLDEPTNNLDPASREQVLEALRRFAGAVVLVTH
                     DPGAVEA"
     gene            1668..2456
                     /locus_tag="VFE39_17170"
     CDS             1668..2456
                     /locus_tag="VFE39_17170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6260370.1"
                     /translation="MTDLTDLSGRTALITGASRGIGFAIAGELLSRGASVTITARKPD
                     ELAAAAEKLVADGAGGDASRVLAVAGNAGSAESREEAVAKTVETFGGLGILINNTGIN
                     PIYGSLMDADLGAVRKIFDTNVVAALGFVQLAYRAWMREHGGAVVNLASTAGLRSTGM
                     IPVYGASKAALIRLTEELAWQLGPGIRVNAVAPSVVKTRFASALYAEGEEKVSAAYPM
                     KRLGEPEDVAALVGFLVSDAASWITGETVRVDGGILATGSLGSG"
     gene            complement(2501..3668)
                     /gene="dgt"
                     /locus_tag="VFE39_17175"
                     /pseudo
     CDS             complement(2501..3668)
                     /gene="dgt"
                     /locus_tag="VFE39_17175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
     gene            3815..5017
                     /locus_tag="VFE39_17180"
     CDS             3815..5017
                     /locus_tag="VFE39_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lycopene cyclase family protein"
                     /protein_id="HET6260371.1"
                     /translation="MPRPEADVLVVGGGPAGRALAAESAVRGLRTVLVDPAPDAPWRV
                     TYGCWAAELPSSLPASTVAAAVRGRAVALTCHDLGWDYAVLDVPTLRAHLDRLLDRAG
                     VTVRAGRAVAMAAPDTVELADGTYVRAAVVVDAGGRRQPLRTASAAPRPAAEQTAYGV
                     VVDESVAAGILAAGEALFMDWRPDHGEPGSPTFLYAVPLGGGAVLLEETSLARRSGLP
                     MPVLRRRLLARLARHDIAPPSDAPVERVLFPVDSPRHRTPGVLGFGAAAPLVHPASGF
                     SVATALTLAPRVADALAAHLPADPRAALAAAEAVVWPAATRAVHRFRRIGLEALLRMP
                     PGEVPGFFEVFLGLPERHRWAYLTARDDLRGTVATMNALFARSDWRLRRRLVLPALRP
                     PAQAAPEG"
     gene            5109..5558
                     /locus_tag="VFE39_17185"
     CDS             5109..5558
                     /locus_tag="VFE39_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020670968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET6260372.1"
                     /translation="MSAYRTIVVGTDGSATSFAAVDRAAAVAGDSNAELVIVCAYTPA
                     GRDDTAEAEDALKDEAYLVRGWTPAEETLRTAEERALAAGAGRVRTVAADGAPVEVLR
                     KVVADANADLLVVGNRGLNTLSGRLLGSVPSDAARRAGVDVLIVHTT"
     gene            5712..6539
                     /locus_tag="VFE39_17190"
     CDS             5712..6539
                     /locus_tag="VFE39_17190"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HET6260373.1"
                     /translation="MSVTSTATLGLTAYLDRIGAPGARAADLPTLRRIVAAHTASIAF
                     ENLDAFTGRDVRLDPDGLVAKLVHRGRGGWCFEQNLLLRGALDALGYRTTGLAARVLW
                     GRPADAPVTPRGHMLLRIDLPEGPHVVDVGFGGLTLTGVLALEPEVEQTTPHEPFRLW
                     PDGPGFVMQARIGGDWRPLYRFDLAEQHLVDYEVSSWYLTHHPASHFRSGLMAARPDI
                     DRRYALGGATLSVHHLDGPTERRTLETPAAVRRALEEQFLLDVSELPDLETALARLF"
     gene            complement(6540..6713)
                     /locus_tag="VFE39_17195"
     CDS             complement(6540..6713)
                     /locus_tag="VFE39_17195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260374.1"
                     /translation="MDHRRDTAGLLDVLREAFGAEELGRVHEEIERRAGVPEFVAAME
                     YVQSSELVVPVQP"
     gene            complement(6949..7353)
                     /locus_tag="VFE39_17200"
     CDS             complement(6949..7353)
                     /locus_tag="VFE39_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007024600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET6260375.1"
                     /translation="MSDTTETTVSPDDVVRGAAGMPEHPTQRAEEPSIDDLEEAMRDV
                     VDPELGINVVDLGLVYGIQAEGGVATIDMTLTSAACPLTDVIEEQTRAALTGSAGLVD
                     DIKINWVWMPPWGPDKITEDGREQLRALGFRV"
     gene            complement(7350..7835)
                     /locus_tag="VFE39_17205"
     CDS             complement(7350..7835)
                     /locus_tag="VFE39_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET6260376.1"
                     /translation="MQLEQMYQEIILDHYRTPHGAGLREPFDAESFQINPTCGDEITL
                     RVKLDGDTVSDVSYETLGCSISQASASVLTDLVVGRSVEESLRVLAAFQEMAQGRGQV
                     EPDEDVLGDGVAFAGVARYPARVKCALLGWMAFKDAVVRTPQSGGGTCIDTRDSEEAE
                     S"
     gene            complement(7838..9118)
                     /locus_tag="VFE39_17210"
     CDS             complement(7838..9118)
                     /locus_tag="VFE39_17210"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015785964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET6260377.1"
                     /translation="MTTLEGVNVQKLRADFPILARTVREGRPLVYLDSGATSQRPRQV
                     LDAERAFLEQHNAAVHRGAHQLAEEATDAYESARARIAAFIGADAGEVVFTKNATEGI
                     NLVAYAMGNSSIRTGLGPESERFALGPGDEIVITELEHHANLVPWQELCRRTGATLRW
                     YSITDEGRVDLDSIELTERTKLVAFAHQSNVLGTILPVAELVRRARAVGALVLLDACQ
                     SVPHMPVHLGELGVDFAVFSGHKMLGPSGIGVLYGRAELLAAMPPFLTGGSMIELVRM
                     EGTTYAPPPQRFEAGVPMTSQVVGLGAAVDYVVDIGMDAIHAHEMALVQQTLDGLASV
                     PGVRVIGPRDLVDRGGAVAFDVDGVHAHDVGQVLDDRGIAVRVGHHCAWPLHRRFGVA
                     ATVRASFHVYSTPDEVTALVEGVQAAREFFGVVS"
     gene            complement(9118..9903)
                     /gene="sufC"
                     /locus_tag="VFE39_17215"
     CDS             complement(9118..9903)
                     /gene="sufC"
                     /locus_tag="VFE39_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET6260378.1"
                     /translation="MSTLEIKDLHVSVATEDGAKEILKGVDLTIRAGETHAIMGPNGS
                     GKSTLAYAIAGHPKYEITSGEVTLDGEDVLAMTVDERARAGLFLAMQYPVEVPGVSTS
                     NFLRAAATAVRGEAPKLRTWVKEVKSAMSDLDIDPDFAERNVNEGFSGGEKKRHEVLQ
                     LALLKPKIAVLDETDSGLDVDALRVVSEGVNRYREGGETGVLLITHYTRILQHITPDV
                     VHVFAGGRIVESGGPELADELEKSGYVRFTGSAVDAAVAGAAV"
     gene            complement(9936..11168)
                     /gene="sufD"
                     /locus_tag="VFE39_17220"
     CDS             complement(9936..11168)
                     /gene="sufD"
                     /locus_tag="VFE39_17220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET6260379.1"
                     /translation="MTVPNTAPEVAGLAPELAGAKKGQGETPISSASERFTSYDVEAF
                     EVPGGREENWRFTPMRRLRGLHDGTARFDGTAAIEVSAAGSAGAGSAGFPRGVSVETV
                     ARDDARIGAGGVPADRVAAAAWSTFREATVVTLDGSPAPVTVTVTGPGEGATAVGHLQ
                     LRTTPNTSATVVVEYRGSGALADNLEVVLADGSRLTLVVTGEWADDAVHVGAQHMSIG
                     RDAVLRGTSVSLGGDVVRISPTVKYRGPGGDAELAGLGFADAGQHLEQRLLVDHAVPN
                     CTSNVIYKNALQSVKGLSAHTVWIGDVLIRAAAENTRTFEYNRNLVLTEHARADSVPN
                     LEIETGEIAGAGHASATGRFDDEQLFYLQSRGIPEDVARRLVVRGFFGEILNRITLPE
                     LRERLEAAIEAELAVTGA"
     gene            complement(11209..12636)
                     /gene="sufB"
                     /locus_tag="VFE39_17225"
     CDS             complement(11209..12636)
                     /gene="sufB"
                     /locus_tag="VFE39_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003885890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET6260380.1"
                     /translation="MTTAPEALTQEETLATLGRYDFGWADPDAAGATARRGLSADVVA
                     DISALKNEPQWMLDFRLKALDLFERKPMPRWGADLSGIDFDNIKYFVRSTEKQATSWD
                     ELPADIKNTYDRLGIPEAEKARLVAGVAAQYESEVVYHQIREDLEEQGVIFLDTDTGL
                     REHPELFREYFGSVIPSGDNKFSALNSAVWSGGSFIYVPKGVKVDIPLQAYFRINTEN
                     MGQFERTLIIVDEGAYVHYVEGCTAPIYKSDSLHSAVVEIIVKKGGRCRYTTIQNWSN
                     NVYNLVTKRAKAEEGATMEWVDGNLGSKVTMKYPAVWLMGEHAKGEVLSIAFAGEGQH
                     QDAGAKMVHLAPHTSSTIISKSVARGGGRTSYRGLVQVNPRAHHSRSTVKCDALLVDT
                     ISRSDTYPYVDVRNDEVSMGHEATVSKVSGDQLFYLMSRGLTEDEAMAMVVRGFVEPI
                     ARELPMEYALELNRLIELQMEGSVG"
     gene            complement(12633..13301)
                     /locus_tag="VFE39_17230"
     CDS             complement(12633..13301)
                     /locus_tag="VFE39_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET6260381.1"
                     /translation="MEQGPTTASAVAAELALSGPAVRRHLDALIADGEAEVRDASRRG
                     PRGRGRPARHYLLTDAGRTRFGHGYDDLAVAALRYLAEYGGEAAVRGFARSRVESLLG
                     QGAEQVAAAHGRDARADALADVLTSRGYAAQAREAGSGVQLCQHHCPVAHVAAEFPVL
                     CEAETQAFAELLGTHVQRLATIARGDSACTTHVPLEAPDVQRGYTTTTTNSTGTSLRG
                     RQSE"
     gene            13487..15073
                     /gene="mptB"
                     /locus_tag="VFE39_17235"
     CDS             13487..15073
                     /gene="mptB"
                     /locus_tag="VFE39_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol phosphomannose-dependent alpha 1,6
                     mannosyltransferase MptB"
                     /protein_id="HET6260382.1"
                     /translation="MVARELDGARAPVPVVAPPRDPSRAARRLGLTGTLLMAVGALGS
                     GALPVPNPLFGLRLLGLPSRNTTIAIAITYAGIGMLVFAWLWVGKMLRSNGAVAPAPT
                     RAQLARTAVLWAIPLAVAPPMFSRDVYSYLAQSAILARGLDPYVLGPAQALGVDDPLT
                     RSIPTIWRDTPAPYGPLFLWLGRGITALTGNDVVLGLFAHRVLALLGVGLIVWSLPRL
                     ARRCGLDAGLAMWLGAANPLVLFHLVSGIHNEALMIGLMLAGMEIGLRAGPRVLDVQF
                     LLGAVLIVGASAVKVPALLALGFLGTEWARRRGGRLRDVASAAGTLGAVTVVGYAALA
                     LATGLGLGWVSTLDTPSIIRSWLSVSTDLGLIGGQIGILGGLGDHTDSVLLLTRGLGG
                     LGAGILVVMLLLATLRGRVDPVTGMGTGLGAVVLLGPIVHPWYLLWAAIPLAATQGLP
                     RYRRAALAASAVLALLVPPTGADFSFRAYQLPMAILAGILMLVLVLLVQRRQLAGQRG
                     VDVDAWPGRVPTPEPERGPH"
     gene            15085..16014
                     /locus_tag="VFE39_17240"
     CDS             15085..16014
                     /locus_tag="VFE39_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET6260383.1"
                     /translation="MNTPPPAVAVRGLVKRYGRTTAVDGLDLCLPSGSVLALLGPNGA
                     GKTTTVEICEGFLRADAGEVRVLGVDPAGAPDALRARIGVMPQGGGAYPGVRAAEMLR
                     LVAACSAHPLDVPWLTSVLGLDSCARTPYKRLSGGQQQRLALACAIVGRPELVFLDEP
                     TAGMDPQGRRVVWELIQALRRDGVAVLLTTHLMEEAEALADEVVIVDRGRVVAQGSPT
                     ALTSTTEQQELRFRARSGMDVDGLIAALPAGYGAAEPIAGRYVVRGRIDPSVLSTITS
                     WCAGQGALADDVQVARRSLEDVFLELTGRELRS"
BASE COUNT         2119 a         5922 c         5673 g         2419 t
ORIGIN      
        1 agcctcgaca gcgccggggt cgtgggtgac gagcacgacg gcccccgcga agcggcgcaa
       61 cgcctcgagc acctgctcgc ggctcgccgg atcgaggttg ttcgtgggct cgtcgagcag
      121 gaggacgttg gcggcgctcg agacgagccc ggccagcgcg aggcgcgtct tctcaccgcc
      181 cgagagcgtg ccggcgggct gctcgagctg gtcgccggag aacatgaacg acccgagcaa
      241 cgtgcgcagt tgctgttcgg gggtgtcggg cgaggcgtgc cggacgttct cccacacggt
      301 ggcgtcgagg tcgagggtgt cgtgctcctg cgcgtagtag ccggtgcgca gcccgtggcc
      361 gggcagtacc cggccggcgt cgggcgtctc ggtgccggcc agcagccgca gcagcgtggt
      421 cttgccggcc ccgttgagcc cgagaacgac gaccttggcc ccccggtcga ccgcgaggtc
      481 gacgccggtg aacacctcca gcgagccgaa cgccttggag agcccctccg ccgtgagcgg
      541 cgtgcgcccg cacggcgacg ggtcggggaa ccggatcttc gccacgcggt cgttcttccg
      601 ctcgggctcg aggcccgcga gcatctggtc ggcccggcgc gccatgttct tcgcggccac
      661 cgccttggtg gccttcgcgc ccatcttcgc cgcctgcgtg tgcagcaccg aggccttctt
      721 ctcggcgttg gcgcgctccc ggcggcggcg cttctcgtcg gtcgcccgcg cctcctggta
      781 gcgcttccag tccatgttgt agacgtcgac ctcgctgcgc gtggcgtcga ggaaccacac
      841 cttgttgacg accgcggcca gcagctcggt gtcgtggctg atcacgacga gaccgccgtc
      901 gtgcgcggcg aggaacgagc gcagccaggt gatcgagtcg gcgtcgaggt ggttggtggg
      961 ctcgtcgagc agcaacgtgg tggcgctcgg cgctccccct cccccgccgt cggaggcggc
     1021 gaacaggatg cgtgccagct cgacgcggcg gcgctgccca ccggagaggg tggagatctg
     1081 ctggtgcagc acgcggtcgg ggaggccgag gtgcgcgcag atgcgagcgg cctcgctctc
     1141 ggcggcgtac ccgccgagag cggagaagcg ctcctcgatg cgcccgtact cgcgcacggc
     1201 cttgtcgttc tgcgcaccgt cgacgagctc cgccatcgcc gtctgggcct tctccatctt
     1261 ccgcagcatc tcgtcgagac cacgggcaga caggacgcgg tccttggcgg tgacggtcag
     1321 gtcgccctcg cgcgggtcct gcggcaggta accgaccggg ctgttggcgc cgaccgagcc
     1381 ggcgtacggc tcgccctcag ccgcgagcac gcgcatcgaa gtggtcttgc cggcgccgtt
     1441 gcgcccgacc agcccgatgc ggtcgccggg ctggacgcgc aacgtggccc cggacagcag
     1501 gatccgggac ccggcacgca gttccaggtc ggtggcggtg atcacgagga gaactccagg
     1561 gggtccggaa gaggatgtgc gagggccggg gcagccgcta ctcgatccgg atcacccctc
     1621 cagactaccg acccccgcga gcggattcgg gaagtagggt gccgcacatg accgatctca
     1681 ccgacctgtc gggccgcact gccctgatca caggcgccag ccgcggaatc ggattcgcca
     1741 tcgccggtga gctgctctcc cggggtgctt cggtcacgat cacggcgcgc aagcccgacg
     1801 agctggccgc ggccgccgag aagctggtgg ccgacggcgc ggggggcgac gcctcccggg
     1861 tgctggcggt ggcgggcaac gcggggtcgg cggaatcccg cgaggaggcg gtcgcgaaga
     1921 ccgtcgagac cttcggcggg ctcgggatcc tgatcaacaa caccggcatc aacccgatct
     1981 acggctcact catggacgcc gacctcggcg ccgtccgcaa gatcttcgac acgaatgtcg
     2041 tcgccgcgct gggattcgtg cagctggcct accgggcgtg gatgcgcgag cacggcgggg
     2101 cggtggtcaa cctggcgtcc accgccgggc tccgctccac gggcatgatc ccggtctacg
     2161 gcgcgtccaa ggccgcactg atccggctca ccgaggagct cgcgtggcag ctcggaccgg
     2221 ggatccgcgt caacgccgtg gcgccgtcgg tggtcaagac caggttcgcc agcgcgctct
     2281 acgcggaggg cgaggagaag gtcagcgccg cctacccgat gaagcgcctc ggcgagcccg
     2341 aggacgtggc cgcgctcgtc gggttcctgg tgtccgacgc cgcctcgtgg atcaccggcg
     2401 agaccgtgcg ggtcgacggg gggatcttgg ccaccggctc gctgggctcc ggctgaggtt
     2461 cacgcccacc gcgctactgc acccccgcta ctgcaccccc ctacagcacc gccgcgtcgg
     2521 tccagagccg gccggagcgg ccggtcagca cgtcgagcag cgcgctggcc tgaccgtcgg
     2581 tcagggacgc gacgtagtcc accaccgctc ggccgcgggc catccggtcc acctccgcgg
     2641 cggacacgac ctcgtcgagc tcgccccggg ccagctcgac gaggtcgtgc agccggggcg
     2701 gcgtgcgggt ctcaccgtcg tcctcgccga gccagtcgtg cagcgcgccg accaggccgg
     2761 tcagcaccgt ggcctgcccg cgctggtggg aggtgaggtc ggcccgctgc aggacgaacc
     2821 ggtggtgtac gaacttgagc accgccacct cgtgccactg ttcggtcgcg agtgcgacgt
     2881 gtgccgaccg gacgggcgga tcggccagca cccgcacacc gcccacgagc cgtgcagtcc
     2941 tcgaggtcgt ggatggcata ggcgatgtcg tcggcgatgt ccatcacggc cgcctccacg
     3001 gtctgctgcc acggcccgat gggcccggcg aacacctcgc ggctcgcccg caggtcgtcc
     3061 agctccgtgg agtacgccga gaacttggcc gagcccgcgt ccggcgcgga cgacaacggt
     3121 ccgccgccgc gcggcgggag cgccgccgtg ctcgggtgcg ggaccgggtg gcccagccgc
     3181 gcccacgggt acttcaggac cgcggcgcgg acggccgcgg tgaggtccag ccccgtgcct
     3241 ccggggccgt ggagatcgat cgtggtgacg atgcggaagc tctgggcgtt gccctcgaac
     3301 ccgtccggca gaccgagctt ctcgcggccg aggcggtcga gcacctgctc gccgagatgc
     3361 ccgaacggtg ggtgcccgag gtcgtgggcc agtgccccag cctcgacgac atcggcgtcg
     3421 cagccgccca gccggtcgag cacggcggcc gtggcggggt cgcccgtcag gcgctcggcg
     3481 atcgcccgcc ccacctgggc cacctgcagg ctgtgcgtga gcctgttgtg gacgagcaac
     3541 cccgaccccg tcgggctcac cacctgggtc accccggcca gccgggcgaa gaacggcgag
     3601 gccacgatcc ggtcccggtc gacgcggaac gggctgcgcg cgagagctgc gccggtctcc
     3661 gccaccagcg agcgccgggt tgcacgcgcc ccgtggtcga ccgacccggg ctcggccgca
     3721 gggccgctcc ccgttcggct ccccaggtgg tcggtcacgg gctgattcgc cactgcagca
     3781 ggttagttgc cgcacctacc ggtagaacgg gccggtgcct cgccccgaag cggatgtcct
     3841 cgtggtcggc ggcggccccg cgggccgcgc gctggccgcg gagtccgcgg ttcgcgggct
     3901 gcgcaccgtc ctggtcgatc cggcgcccga cgcgccatgg cgggtgacct acggctgctg
     3961 ggccgccgag ctgccctcat cccttcccgc atcgaccgtc gccgccgctg tgcgcggccg
     4021 ggcggtcgcg ctcacgtgcc acgacctcgg ctgggactac gcggtgctgg acgtcccgac
     4081 actgcgcgcg catctcgacc gcctgctcga ccgcgcaggc gtcaccgtcc gggccgggcg
     4141 cgcggtcgcg atggcagcgc ccgacaccgt cgagctcgcg gacgggacgt acgtcagggc
     4201 cgcggttgtc gtggacgccg gtggacgccg gcaaccgttg cgcaccgcga gcgcggcacc
     4261 gcgccccgcg gcggagcaga ccgcctacgg ggtggtggtg gacgagtcgg tggccgccgg
     4321 catccttgca gcaggtgagg cgctcttcat ggactggcgc ccggaccacg gcgagccggg
     4381 ctcgccgacg ttcctctatg ccgtaccgct gggcggcggg gccgtactgc tcgaggagac
     4441 ctcactcgcc cggagatcag gactcccgat gccggtgctg cgacgccggc tgctcgcgcg
     4501 gctcgcgcgg cacgacatcg cgccgccgtc cgacgcgccg gtcgagcgcg tgctgttccc
     4561 cgtcgactcc ccgcgccacc gcacgcccgg tgtgctcggc ttcggcgcag cggctcccct
     4621 cgtgcacccg gccagcgggt tcagcgtcgc gacagcgctc accctcgctc cccgggtggc
     4681 agacgcactc gccgcgcacc tcccggcgga cccccgcgcc gcgctcgcgg cggccgaggc
     4741 tgtggtgtgg cccgcggcga cccgcgcggt gcaccgcttc cgccgcatcg ggctggaggc
     4801 cctcctgcgc atgccacccg gcgaggtgcc ggggttcttc gaggtcttcc tcgggctgcc
     4861 cgagcggcac cggtgggcct acctcaccgc gcgcgacgat ctccgcggca ccgtggccac
     4921 catgaacgcg ctgttcgcgc ggtccgactg gcggctgcgt cgcagactgg tcctgcccgc
     4981 gctgcgcccg cctgcgcagg ccgcgccgga ggggtgaccc cgatcatccg gtgacgggca
     5041 ccggctggcg atgcctcctg ctaccgccgg taacctccgg accaccttcc cccgagccgg
     5101 aggctctcat gtccgcctac cgcaccatcg tcgtcggcac cgacggctcc gccacctcat
     5161 tcgccgccgt ggaccgggcc gctgctgtcg cgggcgacag caacgccgag ctcgtcatcg
     5221 tctgcgccta cacgcccgcc ggccgtgatg acaccgccga agccgaggac gccctcaagg
     5281 acgaggcata cctcgtgcgt ggctggacgc ccgcggagga gacgttgcgc acggccgagg
     5341 agcgcgccct cgccgcaggc gcaggcagag tccgcacggt ggccgccgac ggcgcacctg
     5401 tcgaggtgct gcgcaaggta gtcgccgacg cgaacgccga cctgctcgtc gtcggcaacc
     5461 gggggctcaa cacgctgagc gggcggctgc tcggctccgt cccgtccgac gcagcgcgcc
     5521 gcgcaggtgt ggacgtgctc atcgtccaca ccacctgacg tcccggagcc gggtggcagg
     5581 tgctcgccgg catcgccagg ttgctccgcg ggcccagcgc cggtcctcgg ccgcgccacg
     5641 gaacctgcac ccgtggctgc tccagccccg ctcttgacct cgatgcgggt gcagcctcga
     5701 tgatgtggtt catgagcgtc acgagcacgg ccacccttgg cctcaccgcc tacctcgacc
     5761 ggatcggcgc ccctggtgcg cgtgccgccg acctcccgac gctccgtcgc atcgtggccg
     5821 cccacacggc gtcgatcgcg ttcgagaacc tcgacgcgtt caccggacgc gacgtgcggc
     5881 tcgacccgga cggtctcgtg gccaagctcg tccaccgtgg acgcggcggc tggtgcttcg
     5941 agcagaacct gctgctgcgc ggggccctgg acgcgctcgg gtaccgcacc acgggtctgg
     6001 ccgcccgggt gctgtggggc aggccggccg acgcaccggt gaccccgcgc gggcacatgc
     6061 tgctgcgcat cgacctgccg gagggcccgc acgtcgtcga cgtcggcttc ggcggactga
     6121 ccctcacggg cgtgctggcg ctcgaaccgg aggtggagca gaccacgccg cacgagccgt
     6181 tccggctgtg gcccgacggg cccggattcg tcatgcaggc ccgcatcggc ggcgactggc
     6241 gcccgctgta ccgcttcgac ctcgcagagc agcacctcgt cgactacgag gtgagcagtt
     6301 ggtacctcac ccaccatccg gcgtcccatt tccgcagcgg gctgatggcc gcccgaccgg
     6361 acatcgaccg ccgctacgcg ctgggcggcg ccaccctctc ggtgcaccac ctcgacggcc
     6421 ccaccgagcg caggaccctc gagacgcccg cggcggtccg ccgcgccctg gaggagcagt
     6481 tcctgctcga cgtctccgag ctgcccgacc tcgagaccgc acttgcccgc ctgttctgat
     6541 cagggctgca cgggcaccac gagctcgctg ctctgcacgt actccatcgc cgcgacgaac
     6601 tcgggcacgc cggcacgccg ttcgatctct tcgtggacgc ggccgagctc ctcggcaccg
     6661 aacgcctcgc gcaggacgtc cagcaggcct gcggtgtcgc gccgatgatc cacggcgtga
     6721 cggtggtgta gaggaggtgg ccggtcggat cggcgcggtc cggaagagcg ggaccacctg
     6781 ttcgccatcg gccccgcgtc acgcaggctg ccgagtcggc gggctgaccc ggacggtcgg
     6841 ttgcgcggcg cctcgaaccg gacggcggga gcggcagcga aacgacaaac gacatcctcg
     6901 tccggctcta gcggacgaga atgccgttcg ttggcggacg gaccgcggtc agacccggaa
     6961 gcccagggcc cgcagctgct cgcggccgtc ctcggtgatc ttgtcggggc cccacggcgg
     7021 catccagacc cagttgatct tgatgtcgtc gaccaaaccc gcgctgccgg tcagagcggc
     7081 ccgcgtctgc tcttcgatca cgtcggtcag cgggcatgcc gccgacgtga gcgtcatgtc
     7141 gatggtggcc accccgccct cggcctggat gccgtacacg aggccgaggt cgacgacgtt
     7201 gatgcccaac tcggggtcga ccacatcacg catggcctcc tccaggtcgt cgatcgatgg
     7261 ctcctcggcg cgctgggtag ggtgctcagg catgccggcc gcgccgcgca ccacgtcgtc
     7321 gggactcact gtggtctcgg tggtgtcact catgactctg cctcctcgga atcccgggtg
     7381 tcgatacacg ttccgccgcc gctctgcggg gtccggacga cggcgtcctt gaacgccatc
     7441 cacccgagca gggcgcattt cacccgagcc ggatatcttg ccacgcccgc gaacgcaacg
     7501 ccgtccccga gcacatcctc gtccggctcc acctgcccgc gtccctgggc catctcctgg
     7561 aacgctgcca gcacccgcag cgactcctcg accgagcggc cgacgacgag gtcggtgagc
     7621 accgacgccg acgcctggct gatcgagcag cccagcgtct cgtaggacac gtccgacacc
     7681 gtgtcgccat cgagcttgac ccgcaacgtg atctcgtcgc cgcaggtggg gttgatctgg
     7741 aacgactccg cgtcgaacgg ctcacgcagt ccggcaccgt ggggcgtgcg gtagtggtcc
     7801 aggatgatct cctggtacat ctgctccaac tgcacggcta gctcaccact ccgaagaact
     7861 cgcgcgcggc ctgcacaccc tcgaccaggg cggtgacctc gtcaggtgtg ctgtagacgt
     7921 ggaacgacgc ccgtaccgtg gcagccacgc cgaaccggcg gtgcagcggc caggcgcagt
     7981 ggtggcccac ccgaacggcg atgccccggt cgtcgaggac ctggcccacg tcgtgggcgt
     8041 gcaccccgtc gacgtcgaag gccaccgctc caccacggtc gacgaggtcg cgcgggccga
     8101 tgacccggac acccggcacg ctcgccagcc cgtcgagggt ctgttggacc agcgccatct
     8161 cgtgggcgtg gatggcgtcc atgccgatgt cgaccacgta gtcgacggcc gcaccaaggc
     8221 ccacgacctg cgacgtcatc ggcacgcccg cctcgaaccg ctgaggcggc ggggcgtagg
     8281 tggtgccttc catccgcacc agctcgatca tcgagccacc ggtgaggaac ggcggcatcg
     8341 cggcgagcag ctccgcccgc ccgtagagca cgccgatgcc ggacggtccg agcatcttgt
     8401 gtccggagaa cacggcgaag tcgacgccga gctcgccgag gtgcacgggc atgtgcggca
     8461 ccgactggca cgcgtcgagc agcaccagag cgccgaccgc gcgggcccgg cgcaccagct
     8521 ccgccaccgg caggatcgtg cccagcacgt tggactggtg ggcgaacgcg accagcttgg
     8581 tgcgctcggt gagctcgatc gagtcgaggt ccacccggcc ctcgtcggtg atcgagtacc
     8641 agcgcagggt ggcgccggtg cggcggcaga gctcctgcca cggcaccagg ttggcgtgat
     8701 gctcgagctc ggtgatcacg atctcgtcgc ccggcccgag cgcgaagcgc tccgactcgg
     8761 ggccgaggcc cgtgcggatg ctcgagttgc ccatcgcata ggcgaccagg ttgatgccct
     8821 cggtggcgtt cttcgtgaac accacctcgc cggcgtcggc gccgatgaac gcggcgatcc
     8881 gcgcccgcgc cgactcgtag gcgtcggtcg cctcctcggc gagctggtgc gcgccacggt
     8941 gcacggccgc gttgtgctgc tcgaggaagg cgcgctcggc gtcgagcacc tggcgcggcc
     9001 gctgtgaggt ggcccccgag tccaggtaga ccagcggccg cccctcgcgg acggtgcgcg
     9061 ccagaatcgg gaagtccgcc cgcagcttct ggacgttcac accctcgagg gtggtcatca
     9121 gaccgctgcc cccgccaccg ccgcgtcgac ggccgatccc gtgaaccgca cgtacccgga
     9181 cttctccagc tcatcggcca gctccggccc gccggactcg acgatgcgcc cgcccgcgaa
     9241 gacgtgcacc acatcgggcg tgatgtgctg caggatccgg gtgtagtgcg tgatcagcag
     9301 gacaccggtc tcgccgccct cgcggtagcg gttgaccccc tcggacacga cgcgcagcgc
     9361 gtcgacgtcc aggccggagt cggtctcgtc gagcaccgcg atcttcggct tgagcagcgc
     9421 aagctgcagc acctcgtgcc gcttcttctc gccgccggag aagccctcgt tgacgttgcg
     9481 ctcggcgaag tccgggtcga tgtcgaggtc gctcatggcg gacttgacct ccttgaccca
     9541 ggtgcgcagc ttcggcgcct cgccacggac ggccgtggcc gccgcacgca ggaagttgga
     9601 cgtcgagacg cctgggacct cgaccgggta ctgcatggcg aggaacaggc cggcacgggc
     9661 gcgctcgtcg acggtcatcg cgaggacgtc ctcgccgtcc agggtgacct cgcccgaggt
     9721 gatctcgtac ttcgggtgcc cggcgatggc gtaggcgagc gtggacttgc cggacccgtt
     9781 cgggcccatg atcgcgtggg tctcgccggc gcggatcgtg aggtcgacgc ccttcaggat
     9841 ctccttggcg ccgtcctcgg tcgcgaccga gacgtgcagg tccttgatct ccaaggtgga
     9901 cattggtgtc agtgctccgt gtggttgtgt gcgcgtcagg cgccggtgac agcgagctcg
     9961 gcctcgatcg ccgcttccag gcgctcgcgc agctcgggaa gggtgatccg gttgaggatc
    10021 tcgccgaaga agccacggac gacgagcctt cgggcgacgt cctcggggat accgcggctc
    10081 tgcaggtaga acagttgctc gtcgtcgaac cggccggtgg cgctcgcgtg accggcaccg
    10141 gcgatctcgc cggtctcgat ctccaggttc ggcaccgagt cggcccgggc gtgctcggtg
    10201 agcacgaggt tgcggttgta ctcgaacgtc cgcgtgttct ccgcggccgc gcggatcagc
    10261 acgtcaccga tccacacggt gtgggcgctg aggcccttga cgctctgcag cgcgttcttg
    10321 tagatcacgt tcgacgtgca gttcggcacc gcgtgatcga ccagcagccg ctgctccagg
    10381 tgctggcccg cgtcggcgaa cccgagcccg gccagctcgg cgtcgccgcc ggggccgcgg
    10441 tacttcacgg tcgggctgat ccgcaccacg tcgccaccca aagacaccga ggtgcctcgc
    10501 aggaccgcgt cccggccgat cgacatgtgc tgcgcgccca cgtgcacggc gtcgtcggcc
    10561 cactcgccgg tgacgaccag cgtgagccgc gatccgtcgg ccagcacgac ctccagattg
    10621 tcggccagcg cgcccgaccc gcggtactcc acgacgacgg tggccgaggt gttcggggtg
    10681 gtgcgcagct gcagatggcc cactgccgtg gcgccctccc ccgggcccgt gaccgtgacg
    10741 gtgaccgggg ccggcgagcc gtcgagcgtg acgacggtgg cctcccggaa cgtcgaccac
    10801 gccgcggcgg cgacgcggtc ggctggcacg ccgcccgcgc cgatccgcgc gtcgtcacgt
    10861 gcgacggtct cgaccgacac gccgcgtgga aagccggcgg agccagcacc ggcactgccg
    10921 gccgcgctga cctcgattgc cgcggtgccg tcgaacctcg ctgtgccgtc gtgcaggccg
    10981 cgcagccggc gcatcggagt gaagcgccag ttctcctccc ggccgccggg gacctcgaac
    11041 gcctcgacgt cgtaggaggt gaagcgctcg ctcgccgacg agatcggcgt ctcgccttgc
    11101 cccttcttgg cgccggccag ctcgggtgcg aggccggcga cctcgggtgc ggtattcggc
    11161 accgtcatga ttcgtgaatc cttttccgca ggctccgaag attcccggtc atccgaccga
    11221 gccctccatc tgcagctcga tgagccggtt cagctccagc gcgtactcca tgggcagctc
    11281 gcgcgcgatg ggctcgacga acccgcgcac gaccatcgcc atggcctcgt cctcggtcag
    11341 cccgcggctc atcagataga agagctggtc gccgctgacc ttcgagaccg tggcctcgtg
    11401 gcccatcgag acctcgtcgt tgcggacgtc gacgtagggg taggtgtcgg accggctgat
    11461 cgtgtcgacc agcagcgcgt cgcacttcac cgtggagcgc gagtggtgcg cccgtgggtt
    11521 cacctgcacc aggccccggt aggacgtgcg gccaccgccg cgcgccaccg acttcgagat
    11581 gatcgtcgag gacgtgtgcg gcgcgaggtg caccatcttc gccccggcgt cctggtgctg
    11641 gccctcgccg gcgaacgcga tcgacaggac ctcgcccttc gcgtgctcgc ccatcagcca
    11701 cacggccggg tacttcatgg tgaccttgga gccgaggttg ccgtcgaccc actccatggt
    11761 ggcgccctct tcggccttgg cccgcttcgt caccaggttg tagacgttgt tcgaccagtt
    11821 ctggatcgtg gtgtagcggc agcggccgcc cttcttcacg atgatctcga ccactgccga
    11881 atgcagcgag tcggacttgt agatcggcgc cgtgcagccc tcgacgtagt gcacgtatgc
    11941 accctcgtcg acgatgatca gcgtgcgctc gaactggccc atgttctcgg tgttgatccg
    12001 gaaataggcc tgcagcggga tgtccacctt gacgcccttg ggcacgtaga tgaacgagcc
    12061 gcccgaccac accgccgagt tgagcgcaga gaacttgttg tcgccgctcg ggatgaccga
    12121 gccgaagtac tccctgaaca gctccgggtg ctcgcgcagg ccggtgtcgg tgtccaggaa
    12181 gatcacgccc tgctcctcca ggtcctcgcg gatctggtgg tagacgacct cggactcgta
    12241 ctgtgccgcg acgccggcca ccaggcgggc cttctccgcc tccgggatgc ccagcctgtc
    12301 gtaggtgttc ttgatgtcgg ccggcagctc gtcccaggac gtcgcctgct tctccgtgga
    12361 ccgcacgaag tacttgatgt tgtcgaagtc gatgcccgag aggtcggcac cccagcgcgg
    12421 catcggcttg cgctcgaaca ggtcgagtgc cttgagccgg aagtcgagca tccattgcgg
    12481 ctcgttcttc agtgcggaga tgtcggcgac gacgtcggcg gacaggccgc gacgtgcggt
    12541 ggcgccggcg gcgtccggat cggcccagcc gaagtcatat cggccgagcg tcgcgagcgt
    12601 ctcctcctgg gtgagtgcct caggagcggt cgtcattcgg actgcctccc tctcagggac
    12661 gtgcctgtcg agttcgtggt ggttgtggtg taaccccgct ggacatccgg agcttccagc
    12721 ggaacgtggg tggtgcacgc ggagtcgccc cgcgcgatgg tggccagtcg ctgcacgtgt
    12781 gtgccgagca gctcggcgaa cgcctgagtc tcagcctcgc acagcaccgg gaactcggcc
    12841 gcgacgtggg ccaccggaca gtggtgctgg cacagctgca cgccggagcc cgcctcgcgg
    12901 gcctgtgccg catagccgcg tgaggtgaga acgtccgcaa gcgcatctgc ccgtgcgtcg
    12961 cggccatgcg cggccgcgac ctgctcggcc ccctggccga gcaggctctc cacccggctg
    13021 cgcgcgaagc cgcgcaccgc cgcctccccc ccgtactcgg cgaggtagcg cagcgcggcc
    13081 acggcgaggt cgtcgtagcc gtgcccgaac cgggtgcgcc cggcgtcggt gagcaaatag
    13141 tgccgggccg gtcgcccgcg gccccgggga ccacggcggg acgcatcgcg cacctccgcc
    13201 tcgccgtcgg cgatcagcgc atcgaggtga cgccggacgg caggaccgga cagcgcgagc
    13261 tcggccgcca cggccgacgc ggtggtcggc ccctgctcca tcagcaggcg ggccacagct
    13321 gcgcgggtgc ggccgtcacc gctcgacggc ccctcgtggg cgtcgaccgg cagctgcctc
    13381 ccgcttttca caacaccagt gttgcgtata tgtccggcca gggcaaagga tgggtgctgt
    13441 ccgggtgagc accctcacgt gcagggcggc tcgctacgct gcgggcatgg tcgcacggga
    13501 gctggacggc gcacgcgcac cggttccggt cgtagcgccc ccgcgcgatc cgagccgtgc
    13561 tgcccggcgc ctgggactga ccgggaccct gctgatggct gtcggggcgc tcggttcggg
    13621 tgcgctcccc gtgcccaacc cgctgttcgg gctgcggctg ctgggcctgc ccagccgcaa
    13681 caccacgatc gccatcgcca tcacctacgc cggcatcgga atgctggtgt tcgcctggct
    13741 ctgggtcggg aagatgctcc ggtccaacgg tgccgtcgcg ccggcaccga cccgtgcgca
    13801 gctcgcccgc acggccgtgc tgtgggccat cccgctggcc gtcgcgcccc cgatgttctc
    13861 ccgagacgtc tacagctacc tcgcgcagag cgcgatcctg gcccgcggtc tcgaccccta
    13921 tgtgctgggc ccggcgcagg cgctcggcgt cgacgacccg ttgacccgca gcatccccac
    13981 gatctggcgc gacacgccgg cgccctacgg gccgctcttc ctctggctcg gccgcggaat
    14041 caccgcgctg accggcaacg acgtggtcct gggcctgttc gcccaccgcg tgctggcgct
    14101 cttgggagtg gggctgatcg tctggtcgct cccccggttg gcccgccgct gcgggctcga
    14161 cgccgggctt gcgatgtggc tcggcgcggc caacccgctc gtgttgttcc acctggtcag
    14221 cggcatccac aacgaggccc tcatgatcgg gctgatgctt gccggcatgg agatcgggct
    14281 ccgggccggg ccgcgggtgc tggacgtgca gttcctgctg ggcgccgtcc tcatcgtcgg
    14341 cgcgtccgcg gtgaaggtgc cggcactgct cgcgctcggc ttcctcggca cggagtgggc
    14401 gcgacggcgc ggtgggcggc tgcgcgacgt tgccagcgcc gcgggcacgt tgggcgcggt
    14461 cacggtggtg ggctacgccg ccctcgcgct cgccaccggt ctcggactcg gctgggtttc
    14521 cacgctggac acccccagca tcatccgcag ctggttgtcg gtctccacgg atctcggcct
    14581 gatcggcggc cagatcggca tcctcggcgg cctcggcgac cacaccgaca gcgtgctgct
    14641 gctcacccgc gggctgggcg ggctcggtgc cggcatcctc gtcgtgatgc tgctgctcgc
    14701 caccctccgc gggcgggtcg acccggtgac cggcatgggc accggcctgg gcgccgtcgt
    14761 gctactcggc ccgatcgtcc acccgtggta cctgctctgg gccgcgatcc cgctcgccgc
    14821 gacgcagggc ctgccgcgct accggcgggc cgcgcttgcc gcctccgccg tcctcgccct
    14881 gctcgtcccg cccacgggcg ccgacttcag cttccgcgcc taccagctgc cgatggcgat
    14941 cctcgccggg atcctcatgc tcgtgctggt gctgctcgtc cagcggcggc agctcgccgg
    15001 gcagcggggc gtggacgtcg acgcgtggcc cgggcgggtg ccgactccgg agccggagcg
    15061 cggacctcac tagactcgac ggccgtgaac actccgccgc ccgccgtcgc ggtccggggg
    15121 ctggtgaagc ggtacggacg caccaccgcg gtcgacggac tggacctctg cctgcccagc
    15181 ggatccgtgc tggccctgct cggccccaac ggtgcgggca agaccaccac cgtcgagatc
    15241 tgcgaaggat tcctgcgcgc cgacgccggt gaggtgcggg tgctcggcgt cgatccggcc
    15301 ggcgcgcccg acgcactgcg cgcccggatc ggcgtcatgc cgcagggcgg gggcgcctac
    15361 ccgggcgtcc gtgccgccga gatgctgcgg ctcgtcgcgg cctgctccgc ccacccactc
    15421 gacgtcccgt ggctcacgtc cgtgctcggc ctggacagct gcgcccgcac gccctacaag
    15481 cggctgtccg gcgggcagca gcagcggctc gccctggcct gcgccatcgt cgggcgcccc
    15541 gagctggtgt tcctcgacga acccaccgcc ggcatggacc cgcagggtcg ccgggtggtg
    15601 tgggaactga tccaggcctt gcgtcgcgac ggagtggccg tcctgctcac cacgcacctc
    15661 atggaggagg ccgaggcgct cgccgacgag gtggtgatcg tggaccgcgg gcgcgtggtc
    15721 gcgcagggct cccccacggc gctcacctcg acgaccgagc agcaggagct acgcttccgc
    15781 gcccgctccg gcatggacgt cgacggcctg atcgcggcgc tgcccgccgg ctacggggcg
    15841 gccgagccga tcgccgggcg ctacgtcgtg cggggccgga tcgacccctc ggtgctgtcg
    15901 acgatcacgt catggtgcgc cgggcagggg gccctcgccg acgacgtgca ggtggccagg
    15961 cgaagcctcg aggacgtgtt cctggagctg accggacggg aactgcgctc gtgaaccgat
    16021 tcgcaccagg caccttcacc ccgcagccag gcgccggcgc gccattgcgg atgctcgccg
    16081 cccagtcggg caccgagctg cggctggccc tgcgcaacgg cgagcaggtg ctg
//
LOCUS       DASRSC010000358        14229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2284542,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000358 DASRSC010000000
VERSION     DASRSC010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 14229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14229
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2284542"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VFE39_17245"
     CDS             <1..924
                     /locus_tag="VFE39_17245"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HET6260384.1"
                     /translation="PGLPWHLVGGPTSIGEPLVARQVRRLQPDVVFSPMQTMGSWGRT
                     YGLVLTLHDLIYYRNRTPPPEFAAPIRLLWRLYHLAWWPQRVLLNRADAVVTVSEATA
                     TQIAEHGLTHRPVTVVPNAPDSGLDLPRTPRTDALVYMGSFMPYKDVGTLAGAMPALP
                     DHELHLMSRISPEERARLCALAPGGRLVFHDGASDETYREVLRGATALVSASRDEGFG
                     IPLVEAMALGTPVVVSDIPVFREVGGDAAAYVAPGDVAGFVRAVRELGVPGVWEERSA
                     AARAQAARYSWENSARVLLALLERVAAGRAR"
     gene            complement(935..2128)
                     /locus_tag="VFE39_17250"
     CDS             complement(935..2128)
                     /locus_tag="VFE39_17250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET6260385.1"
                     /translation="MKPLPVKPLRVLIGAETYPPDVNGAARFAERLATGLAGRGHDVH
                     VVAPSATGSRTREIRDGVTVHGIRSHRYFMHSDFQVCMPWEAGPDTAALMEEIDPDVV
                     HTQAHMVVGRGIVKAAYRTGRPLVATNHLMPENLIAYSPIPQVLQRAFYTLAWKDLGR
                     VFGRANVVTAPTPRAVELLVRHAGLVDAIPVSCGIDAARYRAAPQVPGVVPTVLFVGR
                     LDQEKRVDELIRAFAALPQDLPAELEIVGDGARRADWTALAAALGIAERVRFRGFVSE
                     EELLDAYARAAVFCMPGVAELQSLVTLESMAAGLPVVAADAMALPHLVRPGRNGWLYT
                     PGDVPELTTRLALLLSDPAMRRRMGAAGREIVAEHAIGATLSTFEGIYERALGRDRLQ
                     VERAA"
     gene            complement(2183..3187)
                     /locus_tag="VFE39_17255"
     CDS             complement(2183..3187)
                     /locus_tag="VFE39_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260386.1"
                     /translation="MIHLLAGSGIAIALAMFGAVCFAGAAVLQHGAVSAESRGAVGGH
                     AGKALSLRGLRAVTRRPGWLAGLALAGGGSALHAIALVLAPLSVVQPIGVLAVPIAVL
                     LTAVQRGRRPAGGVYVGVLLSVGGVAVFVATAASTAVSTPPPDQATLFASLVVAAIVL
                     LLAGLGLARSGWIRCVACAMAGAAAFGLVSALVRAVSQSVASGDVGPLEPPVLAAVAG
                     IGAAVLVGGWLVQQAFASGPPEVVIACLTVVDPIVAVLLGAVLLGEGAATPAGTSVLL
                     VTAAVTAAAGVVALARHHPDAVAARSARVGPGAAESSLPEPSPTPAAHTRLVQSLYRR
                     "
     gene            complement(3184..3900)
                     /locus_tag="VFE39_17260"
     CDS             complement(3184..3900)
                     /locus_tag="VFE39_17260"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HET6260387.1"
                     /translation="MTTNGGAGHEPGGTTWAGETAVVTCVIAAVLPLGLLHVNGIGVL
                     DPLERTISDYVFLTGGYALLGIAAVALAVASLVLASGLRRATLPDPGPPTALMGSAAA
                     ALVLVAAFPTHHPGTTPGLVSTVHRAAGGWVVVVVPLACWMVARRARTTVAWAAAAPT
                     LTWSAGGAGAVSAFFLMSHLPIVIGGSPGFPLLGGVQRVLYSLVMLVLVATARATRLA
                     VERAPIPASLPSAVELRGAT"
     gene            complement(3996..4673)
                     /locus_tag="VFE39_17265"
     CDS             complement(3996..4673)
                     /locus_tag="VFE39_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260388.1"
                     /translation="MTRTRNLTVLAACVVVSLLAAWYATAQFAPPASPPPTGSVRLGP
                     EPGDDVAGYLARLPAELPPAGVDALALVQFGAELTAPEAVAAAGGTVIDTAVLRVPLP
                     RVQTALRFEPLDPGVAPDTALRTARERAQLQADADAARVGGRAHDVASAEAAALAEPG
                     CRCVLALVVRSDRAGLEAVMARSAVRAVEAAPPGTADRELALAPLLPEQTVRTDPPPD
                     DGPVPPS"
     gene            complement(4687..5571)
                     /locus_tag="VFE39_17270"
     CDS             complement(4687..5571)
                     /locus_tag="VFE39_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020660614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET6260389.1"
                     /translation="MRVDAGLSRLLGLSRTAVAALAEDGRVAVDGRAAGKSDRLTAGS
                     WLEVELPEPEAPAEIAGPAAVVAGLTVLHADDDIVVVDKPVGVAAHPSPGWTGPTVVS
                     GLAALGFRVSTSGAAERQGIVHRLDVGTTGVMVVATSEHAYTVLKRAFKERTVDKRYH
                     AVVQGHPDPSSGTIDAPIDRHPKHDYRFAVVADGKPSVTHYDTIEAFRAASLLDVRLE
                     TGRTHQIRVHLSALRHPCVGDLAYGADPKLAKRLGLERQWLHARSLGFEHPADGRWAE
                     FTSPYPADLARALDLLAD"
     gene            complement(5609..6193)
                     /gene="lspA"
                     /locus_tag="VFE39_17275"
     CDS             complement(5609..6193)
                     /gene="lspA"
                     /locus_tag="VFE39_17275"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET6260390.1"
                     /translation="MSEPASDPTTQSAPGAAAGSKVRLLALIAVLVLAVDIITKVVAV
                     AQLEDRAPVELLGGLVYLQLVRNPGAAFSLATGYTWVLTIVAIAVVVVIVRVARRLRS
                     TGWAVALGLVLGGALGNLTDRLFRAPGPLQGHVVDVVSLFTPDGRAWPVFNLADSSIV
                     SGGVLLVLLALLGRELDGRRTSRKADTEDGRVGE"
     gene            complement(6201..7694)
                     /locus_tag="VFE39_17280"
     CDS             complement(6201..7694)
                     /locus_tag="VFE39_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009080341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HET6260391.1"
                     /translation="MASLEVVLGVVAAVVLLGVLAVRASVRLGLPSLLIYLGIGILLG
                     ESVLGVQFSDAPLTESLGLGALVLILTEGGLTTRWEAVRPALGVGIALSTVAVAVSIA
                     VVAAALHLLLDLDWRAAFLWGAVLSSTDAAAVFSVLRGVGVSPRLAGALELESGMNDA
                     PVVLAVILLASADSFTWVTPLLIVYELAAGALIGAVLGFGGAWALRRAALPATGLYPL
                     AAIAVCVLAYSAGQLAHASGLLATYVAALVLGNADLPHRGGVRSFAEGLGSLAQIGLF
                     VLLGLYASPPRLLNAVVPALVAGVVLIVAARPLSVVAATAPFRLPWREQAFLSWAGLR
                     GAVPIVLALIALIEGAPSAQALVDVVFVLVVVLTLVQGPTLPWVARRLGVARTAEPRE
                     LSVDAAPLDELSADMLQVRVPVGSQLRGVYLGELRLPRGATVSLVVRDEKAFTPTAET
                     RLRERDQLLVVTTAVARRATEERLRAVDQRGRLARWHGGREAKGRRG"
     gene            complement(8690..9442)
                     /locus_tag="VFE39_17285"
     CDS             complement(8690..9442)
                     /locus_tag="VFE39_17285"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6260392.1"
                     /translation="MDAASVWAERQEGRGIPPEILATAQNNPFHHDVDFFVAPPQPVD
                     TLSRRAALELLADGGTVLDIGCGGGAAAFALVGRITHVTGVDQQQDMLDSFVAAAEAR
                     ALPYRTVLGRWPDVAAEAGTADVVVSHHVLHNVVDLPPFVRALAAAARRGVVVEMMTQ
                     HPMAWLDPLWERFHGLHRPPSATTDDAVGVLRELGIQPAVQRWERVSAPRDAAWVARR
                     LCLPEDRVSEVDAILDDLDRSRVAATLSWTVH"
     gene            complement(9507..10088)
                     /locus_tag="VFE39_17290"
     CDS             complement(9507..10088)
                     /locus_tag="VFE39_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005151316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6260393.1"
                     /translation="MSIQQRRERERAQRHELIVRTARELAESEGWEAVTTRRLSERID
                     YSQPVLYSHFTGKDAIIGAVAVQGFAELADALAAARAGAGTTNEAVVAVIRTYLAFAD
                     ANPALYDAMFVLAIDLPFAQAATPAPLQAAFGQLHDVLAPLAGDRDSGVFAEVWWSTM
                     HGLVTLARGGRIPAGAQEQRVAILVDQLTGAPQ"
     gene            10188..10568
                     /locus_tag="VFE39_17295"
     CDS             10188..10568
                     /locus_tag="VFE39_17295"
                     /inference="COORDINATES: protein motif:HMM:NF025453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4267 domain-containing protein"
                     /protein_id="HET6260394.1"
                     /translation="MSVTSIAGALAALVSVGIIAIGTRYVVAPVASARTFGLPDWPRG
                     DAAAWLRLKGIRDIVSGLVILVPLALGQVQVLGWIVLVASITPFGDALVVLRNKGSKI
                     LGYAMHGGTAVAALVIAGLLLIGS"
     gene            10571..10816
                     /locus_tag="VFE39_17300"
     CDS             10571..10816
                     /locus_tag="VFE39_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260395.1"
                     /translation="MSLIVPCFDDSVVVRSGEAEGIGRAPTTVVIAPGVERFEYFGHL
                     ERIAYGEVPPESLLEVEEVYDIYFMNSPAWESARATT"
     gene            complement(10841..11116)
                     /locus_tag="VFE39_17305"
     CDS             complement(10841..11116)
                     /locus_tag="VFE39_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HET6260396.1"
                     /translation="MKVSVRVRPGARADIVGGHWDGPRGPALLVAVRARAVEGFANAA
                     VLAALAEAFGVRRGDVALVSGARGRNKVVAVEGDEAVLAARHTALLG"
     gene            complement(11139..>14229)
                     /gene="ileS"
                     /locus_tag="VFE39_17310"
     CDS             complement(11139..>14229)
                     /gene="ileS"
                     /locus_tag="VFE39_17310"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET6260397.1"
                     /translation="FVASVEARPAGENGSNEFVFYDGPPFANGLPHYGHLLTGYVKDV
                     VPRYQTMRGKHVERRFGWDTHGLPAEVEAEHELGIKDKSEIEEMGVAAFNEACRTSVL
                     RYTDEWRAYVTRQARWVDFDNDYKTLDLDYMESVMWAFKTLWDKGLIYSGFRVLWYCW
                     HDGTPLSATETRMDDVYLDRQDPAVTVGLRLRAPGTDLDNALALVWTTTPWTLPSNLA
                     MAVNPAVEYALVRAASGGRYVLASARLAHYARELGSVEDGELEVLRTMPGSELLGLSY
                     VPPFDFFEGWENAHRILAADYVTTEDGTGLVHIAPAFGEEDKVVTDAAGIETVVPVDS
                     RGEFDSRVAPYAGMQVFDANPRIIRDLRDGAPYAQGVLLRHETYDHPYPHCWRCGNPL
                     IQRAVDSWFVQVTAFRDRMVELNQEITWVPEHVRDGQFGKWLEGAHDWSISRNRYWGS
                     PIPVWVSDDPQYPRVDVYGSLDELERDFGVRPNDLHRPYIDELTRPNPDDPTGHSTMR
                     RVTEVLDCWFDSGSMPFAQVHYPFENRDWFEDHYPGDFIVEYNGQTRGWFYLLHVLVT
                     ALFDRPAFRSCMAHGIVLGDDGQKMSKSRRNYPDVNEVFERDGSDAMRWFLMASPILR
                     GGNLVVTEQGIREGVRQALLPLWNTWYFLSLYARAAGRRGVVRTDSTNVLDRYVLART
                     AALVDDVTAAMDVYDVAGATEHVRDHAEALTNWYVRRSRERFWAGDADAIDTLHTVLE
                     VTARVAAPLLPLTMEKVWQGLTGGRSVHLADWPTSGELPHDAALVTAMDRVRQVASSA
                     LSLRKARGLRVRLPLATLAVAAADVAALEPFTDILRDEVNVKDVVLTTDVAKYGRFEI
                     VVNARACGPRLAGDTQKVIRAVKAGEWTANDDGTVTAAGIELLPGEFTEKLVAAGAED
                     PGTATAALPGSTGLVVLDSAVTPELAAEGTARDVVRVVQQARRDSGLDVSDRIELVLD
                     GPSAVLDAVRTHEEFVAGEVLAVSVAYTDVPDPTLAGAVAAPDGAAAEVRVLVTRTR"
BASE COUNT         2093 a         5186 c         5143 g         1807 t
ORIGIN      
        1 cccggtctcc cgtggcacct ggtcggcgga cccaccagca tcggggagcc gctcgtcgcc
       61 cggcaggtgc ggcggctgca gccggacgtc gtgttctccc ccatgcagac gatgggcagc
      121 tgggggcgca cctacgggct cgtcctgacg ctgcacgatc tcatctacta ccgcaaccgc
      181 actccgccgc ccgagttcgc cgccccgatc cggctgctct ggcggctcta ccacctcgcg
      241 tggtggccgc agcgggtgct gctgaaccgc gccgacgcgg tcgtcacggt ctccgaggcc
      301 acggcaacgc agatcgcgga gcacgggctc acccacaggc ctgtgaccgt ggtgccgaac
      361 gctccggact ccggcctcga cctcccgcgg acgccgcgca ccgacgcgct cgtctacatg
      421 ggctcgttca tgccctacaa ggacgtggga acactcgcgg gcgcgatgcc cgcgctgccc
      481 gaccacgagc tgcacctgat gagccggatc tcccccgagg agcgggcgcg cctgtgcgcg
      541 ctggctccgg gcggtcgtct ggtgttccac gacggcgcaa gcgacgaaac ctaccgcgag
      601 gtgctgcgcg gagcgacggc gctggtctct gcgtcgcggg acgaggggtt cgggatcccg
      661 ttggtggaag ccatggccct gggcacgccc gtcgtggtca gcgacatccc ggtgttccgc
      721 gaggtgggcg gagacgcagc ggcctacgtc gcgccgggtg acgtggcggg tttcgtccgt
      781 gccgtgcggg agctcggcgt gcctggtgtc tgggaggagc gctcggcggc ggcacgggcg
      841 caggccgccc gctacagctg ggagaactcg gcacgggtgc tgctcgccct gctcgaacgc
      901 gtcgcagccg gccgcgcccg ctgaggcgcc ggggtcaggc ggcgcgctcg acctgcagcc
      961 ggtcgcggcc cagcgcgcgc tcgtagatgc cctcgaaggt gctcagcgtg gcgccgatcg
     1021 cgtgctcggc cacgatctcg cgaccggccg cgcccatccg gcgccgcatc gcgggatccg
     1081 agagcagcaa ggcgagccgg gtggtgagct cggggacgtc gccgggtgtg tagagccagc
     1141 cgttgcggcc cgggcggacg aggtgcggca gcgccatcgc gtctgcggcg accaccggga
     1201 ggcccgcggc catcgactcc agcgtgacga gactctgcag ctccgccacc cccggcatgc
     1261 agaacaccgc agcgcgggcg tacgcgtcga gcagctcctc ctcggagacg aacccgcgga
     1321 accgcacgcg ctcagcgatc ccgagagcgg cggccagggc cgtccagtcc gcccgccgcg
     1381 cgccgtcgcc gacgatctcc agctcggccg gcagatcctg gggcagcgcc gcgaacgccc
     1441 tgatcagctc gtcgacgcgc ttctcctggt ccagccgacc cacgaacagc acggtcggaa
     1501 cgacgccggg gacctgcggg gcggcacggt aacgggcggc gtcgatgccg caggacaccg
     1561 ggatcgcgtc gacgagcccg gcgtggcgca cgagcagctc cacggcgcgc ggcgtgggcg
     1621 cggtgaccac gttcgcccgg ccgaagacgc gcccgaggtc cttccacgcc agggtgtaga
     1681 aggcccgctg caggacctgc gggatcgggg agtacgcgat gaggttctcc ggcatgaggt
     1741 ggttggtggc cacgagcggc cgaccagtgc ggtaggcagc cttcacgatg ccgcgcccga
     1801 cgaccatgtg cgcctgcgtg tggacgacgt ccgggtcgat ctcctccatc agcgcggcgg
     1861 tgtccgggcc ggcctcccac ggcatacaga cctggaagtc ggagtgcatg aagtaacggt
     1921 gggagcggat cccgtgcacg gtcacgccgt cacggatctc gcgcgtccgt gatccggtgg
     1981 ccgacggggc gacgacgtgc acatcgtggc cgcgcccggc caggccggtg gcgagccgct
     2041 cggcgaagcg cgccgcgccg ttgacgtcgg gcgggtacgt ctcggcgccg atcaggaccc
     2101 gcagcggttt gacgggcaga ggcttcacag gagaggtcct ctcaggtcag tacagcgttg
     2161 agggaggaac cggcgctggg gatcaccggc ggtacaggga ctgcaccaac cgcgtgtgag
     2221 cggccggggt aggggaaggc tccggcaggg agctctcggc tgcgcctggg cccacccgcg
     2281 cgctgcgggc ggcaacggcg tcgggatggt gccgggccag tgcgacgaca cctgccgccg
     2341 cggtaacggc ggcggtgacg agcagcaccg acgtcccggc gggggtggca gcgccctcgc
     2401 cgagcagcac ggccccgagg agcactgcca cgatcgggtc gacgaccgta aggcaggcga
     2461 tgaccacctc cggcggcccg gaggcgaacg cctgctgcac gagccagccg ccgacgagca
     2521 cggctgcgcc gatgccggcc acagcggcga ggacgggggg ctccagcggc ccgacgtcgc
     2581 cggacgcgac cgactgggac acagcccgca ccagcgccga cacgagtccg aacgccgcag
     2641 cgcccgccat ggcgcaggcc acgcaccgga tccagccgga acgggcgagc ccgagcccgg
     2701 ccagcaggag cacgatcgcg gctacgacga ggctcgcgaa cagggtggcc tggtcgggcg
     2761 ggggcgtgct gacggccgtg cttgcggcgg tggcgacgaa cacggccacc ccgccgacgc
     2821 tgaggagcac gccgacgtac accccgccgg ccggccgacg gccccgctgg accgcggtga
     2881 gcagcaccgc gatggggacg gccagcaccc cgatcggctg gacgaccgac agcggcgcca
     2941 gcaccagcgc gatggcgtgc agtgcggatc cacccccggc cagcgccagg ccggcgagcc
     3001 agcccggacg ccgggtcacc gcgcgcagcc cgcgcaacga cagggccttg cccgcgtgtc
     3061 cgcctacggc cccacgggac tcggcgctga cggccccgtg ctgcaggacg gcggctccgg
     3121 cgaagcagac ggcgccgaac atggcgagcg cgatcgcgat gccggaaccg gcaaggaggt
     3181 gtatcacgtc gcacccctga gctcgacggc ggacgggagc gacgccggga tcggagcgcg
     3241 ttcgaccgcc agccgggtcg cccgggcggt agcaaccagc accagcatca ctagagagta
     3301 gagaacacgt tgaacccctc cgagcagggg aaatccgggt gaaccgccga taacgatcgg
     3361 gagatgactc atcagaaaga aggcacttac cgctccggcg ccgcccgcgg accacgtcag
     3421 ggtgggtgcg gcggcggccc acgcgaccgt ggtgcgggcc cggcgggcca ccatccagca
     3481 ggccagcggc accaccacga cgacccaacc gcccgccgcg cggtgcaccg tggagaccag
     3541 gccgggggta gtgccgggat ggtgggtcgg gaaggccgcc acgagcacca gtgccgcggc
     3601 cgcgctcccc atcagcgccg tgggcggtcc cgggtcgggc agcgtggcac ggcgcaggcc
     3661 gctcgcgagc acgaggctcg cgacggccag ggcgacagcg gcgattccca gcagcgcgta
     3721 gccgcccgtg aggaacacgt agtcgctgat cgtccgctcc agcgggtcga gcaccccgat
     3781 gccgttgacg tgcaggagac ccagcggcag gaccgcggcg atcacgcatg tgacgacggc
     3841 cgtctcgccc gcccacgtcg tgccgccggg ctcatgcccg gcccccccgt tcgtcgtcat
     3901 tcgcccaccg tgccaggccg acgctgagac agacatcagg atccccaccg agtccctccg
     3961 ggggtatccc ctacttgccc tgatcagggg acccttcacg acggcggcac cgggccgtcg
     4021 tcgggcggcg gatcggtccg caccgtctgt tcgggcagca gcggcgccag cgcgagctca
     4081 cgatccgcgg tgcccggcgg cgcggcctcc acggcccgca cggcggaccg cgccatcacc
     4141 gcctccagcc cggcgcggtc ggaccggacg acgagcgcga ggacgcaccg gcacccgggc
     4201 tcggcgagcg ctgccgcctc ggccgaggcc acgtcgtgtg cgcgcccgcc gactcgtgcc
     4261 gcgtccgcgt cggcctgcag ctgcgcgcgc tcgcgggctg tgcggagcgc ggtgtccggc
     4321 gccacccccg ggtcgagggg ctcgaaccgc agcgcggtct gcacccgcgg cagcggcacg
     4381 cgcaggaccg cagtgtcgat cacggtacct ccggcggccg cgaccgcctc gggggcggtg
     4441 agctcggcac cgaactgcac cagcgcgagc gcatcgaccc ccgctggcgg gagctctgcc
     4501 ggcaaccgcg ccaggtaccc ggccacgtca tcccccggct ccggcccgag ccgtaccgaa
     4561 ccggtgggcg gcggggacgc gggcggcgcg aactgcgccg tcgcatacca ggcggccagc
     4621 agggacacca cgacgcaggc cgcgagcacc gtcaggttcc gcgtccgcgt catcggccgg
     4681 caggcatcag tcggcgagca ggtccagcgc cctggcgagg tctgccggat acgggctcgt
     4741 gaactccgcc cagcgaccgt cggcgggatg ctcgaagccg agcgagcgcg cgtgcagcca
     4801 ctgccgttcc aggccgagcc gcttggccag cttcgggtcg gcgccgtagg cgaggtcacc
     4861 gacgcacggg tggcgcaaag ccgagaggtg cacgcggatc tggtgggttc ggcccgtctc
     4921 gagccggacg tccagcagcg acgcggcgcg gaacgcctcg atcgtgtcgt agtgggtgac
     4981 gctgggcttg ccgtccgcca ccaccgcgaa ccggtagtcg tgcttggggt ggcggtcgat
     5041 gggggcgtcg atcgtgccgc tggacgggtc ggggtggccc tggaccacgg cgtggtagcg
     5101 cttgtcgacg gtgcgctcct tgaaggcccg cttcagcacc gtgtaggcgt gctccgacgt
     5161 ggccaccacc atgaccccgg tggtgccgac gtcgagccgg tgcacgatgc cctgacgctc
     5221 ggccgccccc gaggtcgaga cgcggaaccc cagcgcggcc aggccgctga cgaccgtggg
     5281 tccggtccag ccgggactcg ggtgggcggc gacgccgacg ggcttatcga ccacgacgat
     5341 gtcgtcgtcg gcatgcagga cggtgagccc cgcgacgact gcggcaggcc cggcgatctc
     5401 cgcgggcgcc tccggctcag gcagctccac ctcgagccag gaacctgcgg tcagccggtc
     5461 ggacttaccg gcggcacggc cgtccacggc taccctgccg tcctcggcga gtgcggcgac
     5521 cgcggtacgg gagaggccca gcagccggga gagcccggcg tcgacacgca tgccgtcgag
     5581 gccgtcgggc acggggagct ggcgtgtgtc actcaccgac gcgcccgtcc tcggtgtcgg
     5641 ccttccgaga ggtgcggcgg ccgtcgagct cgcggccgag cagggccagc agcacgagca
     5701 acaccccgcc ggagacgatc gaggagtcgg cgaggttgaa caccggccag gcccggccgt
     5761 ctggggtgaa cagcgacacg acgtccacga cgtgcccctg cagggggccg ggtgcacgga
     5821 agagccggtc ggtgaggttg ccgagcgcac ccccgagcac caggcccagg gcgaccgccc
     5881 aacccgtgga ccgcaaccgc cgggccaccc ggacgatcac gaccaccacg gcgatggcaa
     5941 cgatcgtgag cacccaggtg tagccggtgg ccaaggagaa cgcggcaccc gggttgcgga
     6001 ccaactgcag gtagaccagg ccgcccaaca gctcgaccgg cgcgcggtcc tccagctgtg
     6061 cgaccgccac caccttcgtg atgatgtcga cggcgagcac cagcacggcg atcagcgcga
     6121 gcagccggac cttggagccg gcggcggcgc cgggggcgga ttgtgtcgtc gggtcgctcg
     6181 cgggctcgct cacgcctcca tcatccccta cggcccttcg cctcccggcc gccgtgccag
     6241 cgcgcgagcc tgccgcgctg gtcgactgcg cgcagccgct cctcggtggc ccgccgagcc
     6301 actgccgtcg tcacgaccag cagctggtcg cgctcccgca ggcgggtctc cgccgtcggg
     6361 gtgaacgcct tctcgtcacg caccaccaga ctgacggtgg caccgcgcgg cagccgcagc
     6421 tcgcccaggt acaccccgcg cagctgcgag ccgaccggca cgcgtacctg cagcatgtcg
     6481 gcgctcagct cgtccagcgg ggcggcgtcg acggagagct cacggggttc ggcggtgcgt
     6541 gccacaccga gccgccgggc gacccacggc aaggtcgggc cctgcaccag cgtgagcacg
     6601 acgacgagca cgaacacgac gtcgaccagg gcctgcgcgc tcggtgcgcc ctcgatcagc
     6661 gcgatcagtg ccagcacgat cgggacggcc ccgcgcaggc ccgcccacga caggaacgcc
     6721 tgctcccgcc acggcaggcg gaacggcgcg gtcgcggcca ccacggagag cgggcgcgcc
     6781 gcgacgatca gcaccacgcc cgcgacgagt gcgggcacca ccgcgttgag cagccggggc
     6841 ggcgaggcgt agagcccgag cagcacgaac agtccgatct gggcgagcga gccgagtccc
     6901 tcggcgaagg agcgcacccc gccgcggtgc ggcaggtcgg cgttgcccag cacgagcgca
     6961 gcgacgtagg tggccagcag ccccgaggcg tgcgcgagct ggcccgcgga gtaggcgagg
     7021 acgcagacgg cgatcgcggc cagcgggtac aggccggtgg cgggcaaggc ggcccgccgc
     7081 agcgcccatg cgccaccgaa gcccagcacg gcgccgatca gcgcgccggc agcgagctcg
     7141 tagacgatga gcagcggcgt gacccaggtg aacgaatccg ccgaggccag caggatgacc
     7201 gcgagcacaa ccggcgcatc gttcatcccg gactccagct ccagggcccc ggcgaggcgc
     7261 gggctcacac cgacaccgcg cagcacgctg aacacggcag ccgcatcggt ggacgacaga
     7321 accgcccccc acaggaacgc cgcccgccag tcgaggtcca gcagcaggtg gagcgccgcc
     7381 gcgaccacgg caatgcttac cgccaccgcc accgtggaca gcgcgattcc cacgccgagc
     7441 gccggccgca ccgcctccca gcgggtggtc aacccgccct cggtgaggat gagcacgagc
     7501 gccccgagcc cgagcgactc ggtgagcggg gcatcggaga actggacacc gagcacggac
     7561 tcgccgagca ggatgccgat gccgaggtag atgagcaacg agggcaggcc cagccgcacg
     7621 gaagcacgga cggcgagcac gccgagcagc accactgcgg ccacgacgcc gagcacgacc
     7681 tccaggctcg ccatccccac ctccgccccc acacggtatc ggcgcaggtg acggcgctgg
     7741 cgcaggggga cagggcggga aacggggggc tgcccccacc ccggacccgg gcacccgcag
     7801 gatggtgtgc ccgccgatgg gctccctcac ctgctcctat ggtcatcacg acggcgtcgg
     7861 tgaacacggc gcggcccgac ccggtgcgcg cgctcatggc tccgtccacc tggctcggcg
     7921 ccgcgcacct gctcctcgat gcggtagtgg cacggcatac gcgttcgtgc tgctcacggg
     7981 gctcttcttc accgtgctgc tgctgccgtt cgcgctgatc gggctgcccg tgtggatcgt
     8041 gacggcgtgg gtgagcgccg cgctggccgg atcgagtgtg cccggctcag cctgctgctc
     8101 ggcgtgcaga tccggccccc agccgatgcc ggccggtgac cggaacccgc tgcgctacgg
     8161 aggcatcaac cccggcgtgc ggcgccgcgg cgcacaccag ctactggcgc tgccgctcgg
     8221 tgtgctgacg tccgggatca cctatttcct cctttcgggt gctgccgcgc tgctcgcgat
     8281 gccggtgctc acgttcctgg tgccgtcgca gggcgccacg gcgttcggca tcccgttcgc
     8341 cgacaccggc ggcggccgcg cactgctcgc cgggatcggg ctcgtgctcc tgctcctcgc
     8401 gcccaccgtg gtgcgcggcc tcaccgcgct cgacgcgtcg gtcgcccgtg ccggggacgt
     8461 cacggcgagc ggtctcaccg gtccgatcac ggtggaccgc gtcagcggcg acgtggccct
     8521 gttcgacctg gccgggccgg tcgacgtcac cagcaggtcg ggtgtgatca gcggcagggg
     8581 tctgcgcagc acggcggtgc gcgccacgcc gacagccgct ccggcgacat ccgcatccgt
     8641 ccctcccgct gacaccgcgc cgctcggcgt ggacgcgcgg ctgctgcgct cagtgcaccg
     8701 tccaggacag ggtggctgcg acgcgggagc ggtccaggtc gtcgaggatg gcgtcgacct
     8761 cggagaccct gtcctcggga aggcacagcc gacgggccac ccacgccgcg tcccgcggag
     8821 ccgacacgcg ctcccaccgc tgcacggccg gctggatccc gagctcgcgc agcactccca
     8881 ccgcatcgtc cgtggtggcc gacggcgggc ggtgcagacc gtggaagcgc tcccacagcg
     8941 ggtcgagcca cgccatcggg tgctgggtca tcatctccac gaccactccc cggcgggctg
     9001 ccgcagcgag cgcacgcacg aacggcggca ggtcgacgac gttgtgcagc acgtggtggg
     9061 agacgaccac gtcggcggtg ccggcctcgg cggccacgtc gggccagcga ccgaggacgg
     9121 tgcggtacgg gagggcgcgc gcctccgcgg ccgcgacgaa ggagtcgagc atgtcctgct
     9181 gctggtccac accggtgacg tgggtgatcc gccccaccag cgcgaaggcg gcggcccctc
     9241 ccccgcagcc gatgtcgagc accgtgccgc cgtcggcgag cagctcgagc gcagcccgtc
     9301 gcgagagtgt gtccacgggc tgcggtggag ccacgaaaaa gtcgacgtcg tggtgaaacg
     9361 ggttgttctg agccgtggcg aggatctctg gggggatgcc ccggccctcc tgacgctccg
     9421 cccacacgct cgccgcatcc atgagctcga gtatgcgccg cgttcgccca tgtcgatctc
     9481 gggctccgct gcgctcggcc tcccgttcac tgcggggcgc ccgtgagctg gtcgacgaga
     9541 atcgccacgc gctgctcctg agcgccggca ggaatgcggc caccacgggc gagggtgacc
     9601 agcccgtgca tcgtgctcca ccagacctcg gcgaacacgc cggagtcccg gtcgcccgcc
     9661 agtggcgcga gcacgtcgtg gagctgaccg aacgccgcct gcagaggcgc gggcgtcgcg
     9721 gcctgcgcga acggcaggtc gatggcgagg acgaacatgg cgtcgtacag agctgggttg
     9781 gcgtcggcga aagccaggta cgtgcggatc acggcgacca cggcttcgtt cgtggttccc
     9841 gcccccgccc tggcggccgc gagcgcatcg gccagctcgg cgaacccctg cacggcgacg
     9901 gcaccgatga tggcgtcctt gccggtgaag tggctgtaga gcaccggctg gctgtaatcg
     9961 atccgctcgg agagccgccg ggtcgtgacc gcctcccagc cctcggactc ggcgagctcg
    10021 cgggccgtcc tgacgatcag ctcgtggcgc tgggcacgct cccgctcgcg gcgctgctgg
    10081 atggacacgc gctgaatcct agcactgcta gtcaatcgat cagtgctcat gatagcgtcg
    10141 aattcagatc tagcttcgct aggtcgagat ctcgacagag gagagccatg tccgtgacgt
    10201 ccatcgccgg cgccctcgcc gctctggtca gcgtcggcat catcgccatc ggaacgcgtt
    10261 acgtcgtggc accggtggcg agcgcgcgga ccttcggcct gcccgactgg ccgcgcggcg
    10321 acgccgcggc ctggctgcgt ctgaagggca tccgggacat cgtgtcgggg ctcgtcatcc
    10381 tcgtcccgct cgcgctcggg caggtccagg tgctcggctg gattgtgctg gtcgcgtcaa
    10441 tcaccccgtt cggtgacgcg ctcgtcgtgc tccggaacaa ggggagcaag atcctcgggt
    10501 acgcgatgca cggcggcacg gccgtggctg ccctcgtgat cgccggcctt cttctgatcg
    10561 ggagctgacc gtgtcgctga tcgttccctg cttcgacgac tccgtggtcg tccgctccgg
    10621 cgaggccgag gggatcgggc gggccccgac caccgtggtc atcgcgccgg gtgtcgagcg
    10681 gttcgagtac ttcgggcacc tcgaacgcat cgcctacggc gaagtaccgc ccgagagcct
    10741 cctggaggtg gaggaggtct acgacatcta cttcatgaac agcccggcgt gggagtccgc
    10801 tcgcgccacg acctgacgac gcggggacgg gtcgctcccg tcacccgagc agcgcggtgt
    10861 gccgggccgc cagcacggcc tcgtcgccct cgaccgcgac gaccttgttc cgaccacggg
    10921 ctccggacac gagcgccacg tcgccccgac ggaccccgaa cgcctcggcg agcgcggcca
    10981 gcaccgcggc gttggcgaag ccctccaccg ctctcgcccg caccgccacc agcagcgcgg
    11041 gtccgcgcgg tccgtcccag tgcccgccga cgatgtcggc gcgggcaccg ggccggaccc
    11101 ggacactcac cttcacggga gcagcgcgac gagcgccgtc agcgggtgcg ggtgacgagg
    11161 acgcgcacct ccgccgccgc tccgtcggga gcggcgactg ccccggccag ggtggggtcg
    11221 gggacatcgg tgtaggccac cgacaccgcc agcacctcac cggccacgaa ctcctcgtgt
    11281 gtgcgcacgg cgtcgagcac cgcggagggg ccgtcgagaa cgagctcgat ccgatcggac
    11341 acgtccaggc ccgagtcgcg gcgggcctgc tgcaccacgc gcaccacgtc gcgcgcggtg
    11401 ccctcggcgg ccagctccgg ggtgaccgcc gagtccagca cgacgaggcc cgtggagccg
    11461 ggcagggccg cggtcgctgt accggggtcc tccgcccccg ccgccaccag cttctcggtg
    11521 aactcgccgg gcaacagctc gatccccgca gcggtgacgg tgccgtcgtc gttggccgtc
    11581 cactcccccg ccttgacggc gcggatcacc ttctgggtgt cgccggccag ccgcgggcca
    11641 caggccctgg cgttgaccac gatctcgaac cggccgtact tcgcgacgtc ggtggtgagc
    11701 acgacgtcct tcacgttgac ctcgtcgcgc aggatgtcgg tgaacggctc cagcgccgcg
    11761 acgtcggcgg ccgccaccgc gagggtggcc agcggcagcc gcacccgcag cccgcgggcc
    11821 ttgcggagcg acagcgccga ggacgccacc tgccgcacgc ggtccatcgc ggtcaccagc
    11881 gcggcgtcgt gcgggagctc acccgaggtg ggccagtcag cgaggtgcac cgaccgcccg
    11941 cccgtgagcc cctgccagac cttctccatc gtcagcggca gcagcggcgc ggcgacccgt
    12001 gcggtcacct ccagcacggt gtgcagggtg tcgatcgcgt cggcgtctcc tgcccagaac
    12061 cgctcgcgcg agcggcgcac gtaccagttg gtgagcgcct cggcgtggtc gcggacgtgc
    12121 tcggtcgccc cggcgacgtc gtagacgtcc atcgcggcgg tgacgtcgtc gacgagcgcc
    12181 gccgtgcgcg ccagtacgta gcggtcgagc acgttcgtcg agtcggtgcg gaccacgccc
    12241 cggcgccccg ccgccctcgc gtagagcgac aggaagtacc acgtgttcca cagcggcagg
    12301 agcgcctgcc gcacgccctc ccggatgccc tgctcggtga cgaccaggtt gccgccgcgc
    12361 aggatgggcg acgccatgag gaaccagcgc atggcgtcgg agccgtcgcg ctcgaagacc
    12421 tcgttgacgt ccgggtagtt gcgccgcgac ttcgacatct tctgcccgtc gtcgccgagg
    12481 acgatgccgt gcgccatgca cgagcggaac gctggccggt cgaacagcgc tgtgaccagc
    12541 acgtgcagca ggtagaacca gccacgcgtc tgcccgttgt actcgacgat gaagtcgccc
    12601 gggtagtggt cctcgaacca gtcgcggttc tcgaacgggt agtgcacctg ggcgaacggc
    12661 atcgagccgg agtcgaacca gcagtcgagt acctcggtca cccggcgcat cgtcgaatgc
    12721 ccggtggggt cgtccgggtt gggcctggtc agctcgtcga tgtacgggcg gtgcaggtcg
    12781 ttcgggcgca caccgaagtc gcgctccagc tcgtcgagcg agccgtacac gtcgacgcgc
    12841 gggtactgcg ggtcgtccga cacccacacc gggatgggcg atccccagta gcggttgcgg
    12901 gagatcgacc agtcgtgggc gccctccagc cacttgccga actgcccgtc gcggacgtgc
    12961 tccggcaccc aggtgatctc ctggttgagc tccaccatgc ggtcgcggaa cgccgtcacc
    13021 tgcacgaacc acgagtcgac ggcccgctgg atgagcgggt tgccgcagcg ccagcagtgg
    13081 gggtacgggt ggtcgtaggt ctcgtggcgc agcagcacgc cctgcgcgta cggagccccg
    13141 tcacgcaggt cacggatgat ccgcgggttg gcgtcgaaca cctgcatgcc cgcgtagggg
    13201 gccacccggg agtcgaactc accacgggag tccaccggaa cgaccgtctc gatgcccgcc
    13261 gcgtcggtga cgaccttgtc ctcctcaccg aaggcgggcg cgatgtgcac gagcccggtg
    13321 ccgtcctcgg tggtgacgta gtcggcagcc aggatccggt gcgcgttctc ccagccctcg
    13381 aagaagtcga acggcgggac gtaggacagc cccagcagct cgctgcccgg catcgtccgg
    13441 agcacctcga gctcaccgtc ctcgacggag ccgagctcgc gcgcatagtg ggccagccgc
    13501 gccgacgcga ggacgtaacg cccgccggag gcggcgcgca ccagcgcgta ctcgactgcg
    13561 gggttgaccg ccatggccag gttggagggc agcgtccacg gggtggtggt ccagacgagc
    13621 gccagcgcgt tgtccaggtc ggtgccagga gcgcgcagcc gcaggccgac cgtgaccgca
    13681 gggtcctgcc ggtccaggta gacgtcgtcc atcctggtct cggtggccga cagcggcgtg
    13741 ccgtcgtgcc agcagtacca gagcacccgg aatccggagt agatcaggcc cttgtcccac
    13801 agggttttga aggcccacat gacgctttcc atgtagtcca ggtcgagcgt cttgtagtcg
    13861 ttgtcgaagt cgacccagcg ggcctggcgg gtgacgtagg cgcgccactc gtcggtgtag
    13921 cgcaggacgg aggtgcggca ggcctcgttg aaggccgcta cgcccatctc ctcgatctcg
    13981 gacttgtcct tgatcccgag ctcgtgctcc gcctcgacct cggccggcag tccgtgggtg
    14041 tcccagccga accggcgttc gacgtgcttg ccgcgcatcg tctggtaacg cggcacgacg
    14101 tccttgacgt agcccgtgag caggtgcccg tagtgcggca ggccgttggc gaaggggggc
    14161 ccgtcgtaga agacgaactc gttgctgccg ttctcaccag cgggacgcgc ctcgaccgac
    14221 gcaacgaag
//
LOCUS       DASRSC010000359        24157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2119390,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000359 DASRSC010000000
VERSION     DASRSC010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 24157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24157
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2119390"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VFE39_17315"
     CDS             <1..218
                     /locus_tag="VFE39_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260398.1"
                     /translation="YTGLGVRRQQAGHTVMIAAHTSFAPLVCAAGLGFHDLAGDIGVL
                     TSPLAERVGTEDGSGAVVQAVDAAAAR"
     gene            complement(841..2091)
                     /locus_tag="VFE39_17320"
     CDS             complement(841..2091)
                     /locus_tag="VFE39_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6260399.1"
                     /translation="MPPLPTSEIERVFSEEYGRAVAVLVRVFGDIDVAEEAVQDAFTA
                     AVRRWSSTGLPPSPAGWIITTARNRAIDRLRREASRDDRHAQAALLHARADGSTAGDP
                     AQASTAEESTVRDDRLRLIFTCCHPALGAGVRVALTLRLLGGLTTAEIARAFLVSEST
                     MAQRLVRAKGKIRDARIPYRVPNEADLPDRLRAVLAVVYLIFNEGYTASSGERLVREE
                     LCADAIRLSRLLAELMPDEPEVMGLLALMLLVESRRCTRITPDGDLVLLADQDRSLWD
                     HGLIAEGQAIVRRCLRRNQPGPYQIQAAINAVHSDAPTAAATGWGQIVALYDQLLPLA
                     PSPVVALHRAVAVAEVDGADAALTLVDRLGLDRHHLFHAIRADLLRRLGRDTEAVQAY
                     DAAIARAENAAERTFLQRSRRALA"
     gene            2406..3842
                     /locus_tag="VFE39_17325"
     CDS             2406..3842
                     /locus_tag="VFE39_17325"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET6260400.1"
                     /translation="MTVDVVTRDGTQERVSDDAMAELTARLRGPLITADDPAADVEPV
                     PVWNAMHVDRPAVTARCTGTTDVVEAVTFAREHGLLLAVRGGGHSVAGFSTVRDGMLV
                     DLSSMRGVQVDPGRRLVRVQGGALLADVDRETQAFGLAAPLGRVSETGVAGLSLGGGY
                     GHLQTRLGLSCDNIVEAQVVCADGRIRIASAGSNPDLFWAIRGGGGNFGIVTSFTFRL
                     HPVGPVVAFAGTFYPLEDLPDVERRWRDHVVIAPDQVTSFIVTMTFPAAPEMPEVIHN
                     RPVAIVGGVYCGDPDDGMRVMQPLRELGTPLFDMSQPMPYTAVQSSFDPLFQRRSLRA
                     YWKSQYLDELTDDAIEAIAGRALDRPAPLSMVNTFHIGGAVHAVGPEETSFAERSAPF
                     MVSIDTNWSDPAQDSAAIAWGRDAWEEMTKYGNGNVFLNFTGLADESVRAGVDSAFGR
                     NLRRLGQIKATYDPDNLFQVNNNIMPAQ"
     gene            4189..4680
                     /gene="ectA"
                     /locus_tag="VFE39_17330"
     CDS             4189..4680
                     /gene="ectA"
                     /locus_tag="VFE39_17330"
                     /EC_number="2.3.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033816 - diaminobutyrate
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019491 - ectoine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate acetyltransferase"
                     /protein_id="HET6260401.1"
                     /translation="MSLGRPRVADGVACWNLAAASRVLDVNSRYAYLLWFRDFAATSI
                     VARLDGEIAGFVTGFRRPDEPRTLVVWQVAVAESARGCGVAGAMLDALFDAVPYVNHL
                     ETTITPDNGGSIALFIGFARRRFAEIRRTELFGSDLLGADHEPEILFRIGPIDRRNHW
                     DDV"
     gene            4680..5939
                     /gene="ectB"
                     /locus_tag="VFE39_17335"
     CDS             4680..5939
                     /gene="ectB"
                     /locus_tag="VFE39_17335"
                     /EC_number="2.6.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045303 - diaminobutyrate-2-oxoglutarate
                     transaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HET6260402.1"
                     /translation="MTDFDVESQVRSYCRSWPVVFDTARGARLTDVDGRSYLDFFAGA
                     GALNYGHNPPALKEPLLEYLGRDGITHGLDMYTVAKGEFLRTFEELVLRPRGLNYKVQ
                     FPGPTGANTVESALKLARKIAGRESIINFTHAFHGMTLGALSVTGNSMKRGGAGIPLV
                     HATPMPFDNYFDGTVPDFLWLDRLLSDSGSGLNEPAGVIVETVQGEGGINPARIEWLQ
                     GLADLCRRHHILLIVDDVQMGVGRTGPFFSFEIAGIEPDIVCLSKSISGYGLPMALTL
                     IKPEYDQWGPGEHNGTFRGNNPAFVTATAALREYWSDDALERSTLAKGERVEAALTEL
                     ADSSQAVELTPKGRGLARGLSFSHPGMAAKTCAAAFERGLLMETAGPSDEVMKIMPPL
                     TISNAELEEGLAVVGDAVRTVTGGVVA"
     gene            5936..6343
                     /locus_tag="VFE39_17340"
     CDS             5936..6343
                     /locus_tag="VFE39_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine synthase"
                     /protein_id="HET6260403.1"
                     /translation="MIVRTLDGITDTERDVRTEHWRSKRLVLAGDGVGFSLHETVLDP
                     GSVNDFWYANHIEAVFVTEGEGELYDKDNDVTYRLAPGSVYVLNGNEHHQLRPRTRMR
                     TVCVFNPPVTGREVHDENGVYPLIILSAEEEAS"
     gene            6343..7248
                     /gene="thpD"
                     /locus_tag="VFE39_17345"
     CDS             6343..7248
                     /gene="thpD"
                     /locus_tag="VFE39_17345"
                     /EC_number="1.14.11.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectoine hydroxylase"
                     /protein_id="HET6260404.1"
                     /translation="MTVLDAPTTDRYPTRIADRPALIQRGEPTVWGGTDAPGPLSRDE
                     LTAHDRDGFVTVPELLTPGEVASYAAELDRLAADPGIRADERTIVEKSSQQVRSIFEV
                     HRISEAVAALVADERVAGRARQILGSDVYVHQSRINYKPGFGGGGFYWHSDFETWHAE
                     DGMPVPRAVSISIALTENYPHNGCLMIMPGSHRTFVACVGETPADHYKESLREQEIGT
                     PDPDSLTTLADRHGIAMLTGPAGSATLFDSNCMHGSASNITPYSRSNIFVVFNSVENG
                     LTEPYAAPSPRPSYIATREIAPITG"
     gene            complement(7253..8032)
                     /locus_tag="VFE39_17350"
     CDS             complement(7253..8032)
                     /locus_tag="VFE39_17350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HET6260405.1"
                     /translation="MAWNSFVALGDSFTEGLDDWRPDGSPRGWADRVAECLGANRPAF
                     RYANLAVRGKLLDQIVADQLPVAERLRPDLIAFCAGGNDIIRFTCDTDDLARRFDSAL
                     ERVTATGAQVFVFTGFDLGRMHPIIRRLRGRVACYNELMRASAERHGSTVVDLWGMAS
                     LADPRTWGSDRLHLTAEGHRRVALRVLETLGEPVAEDWRAPLPEAQPSPWRHRQREDL
                     LWMRDFVMPYVRRRLRGDKTGDGFAPKRPELLPLDGGQPAL"
     gene            8195..8878
                     /locus_tag="VFE39_17355"
     CDS             8195..8878
                     /locus_tag="VFE39_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3159 domain-containing protein"
                     /protein_id="HET6260406.1"
                     /translation="MARCNPKPDASLAEILGGRSGALEATVPVVAFVAVFTLVDAAAW
                     AWPVAWAGGAAVLAAAAIGGVRLAGGRRPRAVLFGLLGVAAAALVALYTGRAVDFFLV
                     QIVSNAASALAWAVSIVIRWPLLGLVVGTVLGQHSRWRRDPHLLRGYQRASWVWVGQY
                     LVRVAVFLPLYFADAVFALGVARSVLTWPLVALSVALSWPLIRSALPPGHRGLRHPAE
                     GSPGSRSST"
     gene            9117..9992
                     /locus_tag="VFE39_17360"
     CDS             9117..9992
                     /locus_tag="VFE39_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260407.1"
                     /translation="MQARPKGGLDLTVNKVIAGAGAAATSAVLGSFFGAAGTVTGAAL
                     GSVASTVASTVYQRSLDRTRDRLVTRIRLTGRHGAQGAQDQATQVMMPVPRVSPEGVT
                     ARVRVEPAVPARPSKRRFVLWSAATVLVFVLGLLAVTGIEWAKGSTLTSGESGTSVGR
                     VIEGNHSSRHRVEPVDPTPTTTPEPSSTPDPSADPSTSSSAEPSVGPTPSNGRDSGSP
                     TSAPAAPGAGSGGNDGSGNGGSGNGGSGNDGSGNGGSGNDGSGATGDPGSGSGGQGGG
                     SVPTPLVPVPGGPGN"
     gene            complement(10145..10813)
                     /gene="thiE"
                     /locus_tag="VFE39_17365"
     CDS             complement(10145..10813)
                     /gene="thiE"
                     /locus_tag="VFE39_17365"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET6260408.1"
                     /translation="MPGLDGDALRARLADARLYLCTDARRRTGDLAEFAEAALAGGVD
                     IVQLRDKGADGALEAREELAALEVLAEACARHGALLAVNDRADVAVAAGADVLHLGQD
                     DLPIEWARKIVGDDVVVGRSSHSAEETATAAAEAGVDYFCVGPCWPTPTKPGRPAPGL
                     DLVRTVAGRSPSRPWFAIGGIDHDRLDEVLGAGADRIVVVRAITDSDDPRAAAATLAA
                     RLRG"
     regulatory      10861..10968
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10960..12132
                     /gene="thiO"
                     /locus_tag="VFE39_17370"
     CDS             10960..12132
                     /gene="thiO"
                     /locus_tag="VFE39_17370"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HET6260409.1"
                     /translation="MKNSRLTVIGAGVIGLSCAWRAAAAGLRVTVLDPAPASGASWVA
                     GGMLAPVTEAWPGEEALLELGVESVRRWRGFAEEVGAAAGRPAGLRTEGTVVVATGAG
                     DRAELDTLAGYLARLGRDVERLSGRELRRLEPALGPDVRGGLSVPDDLAVDNRVLLAA
                     LRVAGEGAGVTFVERAARAVLDDGTRVTGVRTDGADLASDVVLICAGAHSGSLHPVLH
                     GLVRPVKGEILRLAHRSGAFPPPTRTVRALVDGRPVYAVPRDGGGLVIGATQAETGFD
                     TEVTVGGVRDLLRDAERVLPGIAEYALVESAAGLRPGSLDNLPLIGALGPDGLLVATG
                     HHRNGMLLAPVTADAVLELLHGRPVPEAVRAADPDRCVSGANRHHEEVARARVDQR"
     gene            12110..12310
                     /gene="thiS"
                     /locus_tag="VFE39_17375"
     CDS             12110..12310
                     /gene="thiS"
                     /locus_tag="VFE39_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HET6260410.1"
                     /translation="MHVWINGEQRELEAGASVLDALTALGAPSSGVAVAVDGEVVPRA
                     GWSGTGLVDGARIEVLTAVQGG"
     gene            12313..13086
                     /locus_tag="VFE39_17380"
     CDS             12313..13086
                     /locus_tag="VFE39_17380"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010308431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HET6260411.1"
                     /translation="MTIDTGLLVIGDRKIASRLIMGTGGAANLEILERALVASGTSLT
                     TVAMRRIDAATGTGVLDLLRRLDIEVLPNTAGCRTAAEAVLTARLAREALETDLVKLE
                     VVADERTLLPDPIELLDAAEQLVDDGFTVLPYTNDDPVLARKLEQVGCAAVMPLGSPI
                     GTGLGIRNPHNIEMIVAAASVPVVLDAGIGTASEAAQAMELGCDAVLLATAVTRAGDP
                     ERMAHAMRLAVEAGRAARGAGRIPRRYWAQASSPDPDAS"
     gene            complement(13114..13653)
                     /locus_tag="VFE39_17385"
     CDS             complement(13114..13653)
                     /locus_tag="VFE39_17385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005441708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HET6260412.1"
                     /translation="MPLFSFEGLSPTVHPDAFIAPTATLVGDVRVEAHASIWYNAVLR
                     ADFGPIIIREGANVQDGCVLHGGDDPITEVGAGATIGHLCVVHGCVIGPEALIGNGST
                     IQDGARIGARCLVGAGATVAPNTVVPDGQLVLGTVARVKGPLTESARWWVEGNPGIYQ
                     ELARRHRAGVTPVVPAFDC"
     gene            13777..14634
                     /locus_tag="VFE39_17390"
     CDS             13777..14634
                     /locus_tag="VFE39_17390"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HET6260413.1"
                     /translation="MIGRRGTPLVLAVLLAGCGSAPLSSSQPSALATPVSSLPSSPPA
                     GSAGEAAALPIYYVAETPAGFRLLREFHRVPSADPGSDAAREMLANPTGTDPDYRSHW
                     PPGTTLRTPVRTDGGTIVVDLSGADAARVDAELAEPTVQQLVFTVQGALQSTAPVRIL
                     VDGEPVDQLWGAVSTAEPVERGDDYALRSLVQIDAPAHGATVGREVEVRGEAAVFEAT
                     VGWEVLRDGAVVRSGFTSTTEGQRFAPFAFAVTLEPGEYVVRVLEDDPSGGQGRPVLT
                     DDKAVTVRP"
     gene            complement(14663..15274)
                     /locus_tag="VFE39_17395"
     CDS             complement(14663..15274)
                     /locus_tag="VFE39_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260414.1"
                     /translation="MRFYAERPARVALQLLVDLLAIGWLVLWVLVAGTARDLILGLQG
                     PGRSLIGAGDAIRGAFDDAARTAQPVPFVGDDLARALGTGTGAGESLAAAGREQVETV
                     ATVASGAAIAIVLLAAMPVLLVWLPMRVRYARAARSAIAVRSVDSDLLALRAMARLPV
                     RRLLAVSPDPAAAWRRDDRSVVHGLAALELRSLGLRTPARPPD"
     gene            complement(15407..16519)
                     /locus_tag="VFE39_17400"
     CDS             complement(15407..16519)
                     /locus_tag="VFE39_17400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HET6260415.1"
                     /translation="MRDVVGQLEGWWNKGETAALATVVGTYSSAPRQPGASMLVGPGG
                     EAVGSVSGGCVEGAVYELGHQVLTDGRPVLQRYGVSDDDAFAVGLTCGGIIDIFVEKI
                     DQDSYGQLGAVAEAIRSESPVAVATVVSGPAERLGKRLVVWPDRTEGGTGSARLDDAV
                     RDDARGLLDAGRNATLHYGVDGQRRGEGLDVFVESFAPPPRMIVFGAIDFAAAVAKIG
                     SFLGFRVTVCDARPVFATASRFPGANEVVVEWPHRYLAAEAEAGRIDARSVLCVLTHD
                     PKFDVPLLEVALRLPEVGYIGAMGSRRTHEDRLARLREAGVTEAEIGRMSSPIGLDLG
                     ARTPEETAVSIAAEMIALHWGGAGARLAEREGPIHV"
     gene            complement(16512..17645)
                     /locus_tag="VFE39_17405"
     CDS             complement(16512..17645)
                     /locus_tag="VFE39_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET6260416.1"
                     /translation="MTTSTPAPTDDPVPGLVGFTVVLRNAGLAITTDRVATFLAALDA
                     LDVTSRVQTYWAGRLTLCSDPDDLPRYDRAFEDWFTPPSGGRTQITDNRPPPPKLAAL
                     TPSAEDGESDDDGREDPLIRARASGTEVLRNRDLAELSPAEREHLRRLLALLHPELPA
                     RVSRRMRPARHGGTDPGATLRAALRNQGELRELRRRDHSRRPRKIVLLVDVSGSMEPY
                     ADALLRFAHVVVRRSPGAVEAFTLGTRLTRVTRELRMRDAERALGAAAKAIPDWSGGT
                     RLGEVLQAFVDRWGQRGAARRAVVVVFSDGWERGSTDLLGQQMARLRRLAHRLVWVNP
                     HVGKEGYAPVQGGIVAALPYLDDLLAGHSLATLERLLEVVRDA"
     gene            complement(17652..18551)
                     /locus_tag="VFE39_17410"
     CDS             complement(17652..18551)
                     /locus_tag="VFE39_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HET6260417.1"
                     /translation="MTDRPNIAGSPVELAAALRSTGYLADDGVATATFLAIQMNRPLF
                     CEGEPGTGKTALAHALAEVFGAELIRLQCHDGIDASQALYDWDFPRQLLHLRALEAAA
                     GASAERLDADSVESSLYDPRFLLARPILRALQSSPSVLLIDEIDRADDEFEAFLLEVL
                     TEHAVTIPEVGEIRAKVPPLVVLTSNRTREVHDALKRRCLYLWLDHPDLQREIEILHS
                     RLPGLPERLAAQVAGAVQQLRRADLLKPPGVAETIDWARALQLVGARHLDVDSAAATL
                     GAVLKYREDADRVRKQLDTLLAS"
     gene            19041..19691
                     /locus_tag="VFE39_17415"
     CDS             19041..19691
                     /locus_tag="VFE39_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HET6260418.1"
                     /translation="MENNEMTSRPDSRKGHKLLRLAVAGAVVVGVPLAVAGTANAAPD
                     SAWDRLAQCESGGNWNINTGNGFSGGLQFTPSTWRAFGGTGSANQASREQQIAVAERV
                     LATQGWNAWPVCSVKTGVRGNTAEVRVSAPVATRIEAPAKAEVKNKVQAPAKARTAAP
                     RLTKNGANYTVVKGDTLSKIAAERRLPGGWQAIYQRNTDVLSNPNVLSIGQQLDLA"
     gene            complement(19759..20202)
                     /locus_tag="VFE39_17420"
     CDS             complement(19759..20202)
                     /locus_tag="VFE39_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018332366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HET6260419.1"
                     /translation="MTDVTNGAAVLRAAVGDAPLDVAEHAALVDQAAAGAVVTFAGVV
                     RDHDGGRSVQGLEYSAHPTAEKVVAEVAAEIAAHARGVRAIAVSHRVGRLAIGDVALA
                     CAVAADHRREAFETCAELVEEVKRLLPVWKHQVFTDGTEEWVGST"
     gene            complement(20199..20684)
                     /locus_tag="VFE39_17425"
     CDS             complement(20199..20684)
                     /locus_tag="VFE39_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HET6260420.1"
                     /translation="MSPADRRARVVTASNRAAAGVYADRGGPLIVDWLRERGFETPDP
                     AVVPDGEPVGDALRAAVADRVDVVITTGGTGISPTDATPEITRAVLDYEVPGLADAIR
                     AAGAPTVPTAVLSRGLTGVAGRTLVVNLPGSTGGVRDGLSVLADVLEHAVDQLRGGDH
                     R"
     gene            complement(20681..21160)
                     /gene="moaC"
                     /locus_tag="VFE39_17430"
     CDS             complement(20681..21160)
                     /gene="moaC"
                     /locus_tag="VFE39_17430"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HET6260421.1"
                     /translation="MELTHVDAAGAARMVDVTDKEPTARTAVATGTLRTTAEVIELLR
                     RDGLPKGDALATARIAGIMGAKRTPDLVPLCHPIALSGVVLDLEPGGAEVRIRATVRT
                     TDRTGVEMEALTAVAVAGLALHDMIKAVDPAAVLDGVRVERKDGGKTGTWTRPEDRS"
     gene            21568..22161
                     /locus_tag="VFE39_17435"
     CDS             21568..22161
                     /locus_tag="VFE39_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010692827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase family protein"
                     /protein_id="HET6260422.1"
                     /translation="MRYRGRHRAPTSTGRTIARTALAGAVAGAPLVIAAPAAHAASDS
                     TWDRVAQCESGGRWDINTGNGFHGGLQFTPSTWKGFGGTEFAPVAYQASRAQQIVVAE
                     RVLAKQGWGAWPVCSRKAGAVGEPPSQRSAPATQVRLSAPTPAATSGSYVVKRGDTLS
                     TIAAQHGVADGWKGLVQKNPGLAGNPNRISPGQHIAV"
     gene            complement(22206..22772)
                     /locus_tag="VFE39_17440"
     CDS             complement(22206..22772)
                     /locus_tag="VFE39_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET6260423.1"
                     /translation="MLAAGAGRRMGGPKALVELDGEPLVRRALRVLAAGGCEPLVVVL
                     GAAAAEVAGIVPSGVTVIVAEDWAEGMGASLRAGLAALEALSPVPDAALIHLVDLPGV
                     TAAAVARLGAGSAPDVLLRAAYDGKPAHPVLIGRSHWSGVRATAIGDAGARAYLAGHP
                     DLAIVECGDLADPDDVDTPEQLARFRSR"
     gene            complement(22790..22969)
                     /locus_tag="VFE39_17445"
     CDS             complement(22790..22969)
                     /locus_tag="VFE39_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260424.1"
                     /translation="MAKTAQQGRIDPSWPELPDGEYPVSELASPLQGSLSPFGEVEFP
                     LEEVPYVHPVTEINK"
     gene            23145..>24157
                     /locus_tag="VFE39_17450"
     CDS             23145..>24157
                     /locus_tag="VFE39_17450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HET6260425.1"
                     /translation="MPTPLVDWLRAQDDETLAVLLRLRPDLAVPPPADLTVLATRAGI
                     RASVHRACDDLDRITLAVLEALVVAGADSAPVPLDEVRRLLGPDVSVTALSGPLTALR
                     DRALAWGPDGELSLVPAVHEVVPRHPGGLGREAAGPAGSSGLPELLAALQPDERRVLE
                     ALAAGPPIGRSRAGSDPDSPVGRLLARGLLLRVDAETVELPRQVGLALRGERPLGEVA
                     ITPPDIGARDRGAHSVDRTAGGAALGVLRQLELLIAFWGRTPPPVLRSGGLGVRELRR
                     AAKEMDADETVAALLMELAVAADLVGESDGVAPEWVPTTNADLWAAGGPELRWSLIAR
                     TWL"
BASE COUNT         3395 a         8558 c         8760 g         3444 t
ORIGIN      
        1 cgtacacggg cctgggggtc cgccggcaac aggccgggca caccgtgatg atcgccgctc
       61 acacctcgtt cgcgccgctg gtgtgtgctg ccggcctcgg tttccacgac ctggccggcg
      121 acatcggcgt gctgacctcc ccgctggccg agcgcgtcgg aaccgaggac ggctccggtg
      181 ccgtcgtgca ggcggtggac gcagccgccg cgcgctgagg ctcgggcgct cagccgttct
      241 gtgccgtccc gcgacgttca ggcgagtgcg ggggcgccgg agacggcgga ggacgtgcgg
      301 ggccggacgc ccggccgtgt gatcgggacg cacgcccccg atgcggtgcg cccggcggtt
      361 tcggcggcgc cggaccacac ggctcaaacg ggcgccgact tcagcagtcc gggcacaccg
      421 ccggtggcga cgacaagacc gccgggcggt ggatcgtcga cacctgcggt cgtcccgtca
      481 ggagggccat acagctgctc gaggtcgacc cacaccggcc ggtccagcgg tggggatgac
      541 tggcgggtgc tggacgacca cgattggtac acatctcctc cgaccgacga aaactcggca
      601 gccgggggaa gcggccacgc atctgctcga cgaagggtct catgccgtgc gccgcccgac
      661 gccgacgcgc gttcgtgcag ggtccgacta ctaccacgcc cggtcgatcg agccggcgcg
      721 gaagcgggca gtcgaggcgg ggtggtcacc cggaccagct tcgaggtggc gaccgcaggg
      781 cttctcgggc agcgatatgc cccgccggct cgccgatccg gtgagctccg cggtcctcgg
      841 tcaggcgagc gcccgccgac tgcgctggag gaacgtgcgt tcggccgcgt tctcggcgcg
      901 ggcgattgcc gcgtcgtacg cctgcaccgc ctcggtgtcg cggcccaagc gtcggaggag
      961 gtcggcgcgg atggcgtgga acaggtggtg acggtcgagg ccgaggcggt cgacgagggt
     1021 gagtgccgcg tccgctccgt ccacctcggc caccgctacc gcgcggtgaa gcgccacgac
     1081 cgggctcggg gcaagtggga ggagctggtc gtacagggcc acgatctgcc cccagcccgt
     1141 ggcggccgct gtgggcgcgt cgctgtggac ggcgttgatc gccgcctgga tctggtacgg
     1201 gccgggctga ttgcgccgga ggcatcgccg gacgatcgcc tggccctcgg cgatgaggcc
     1261 gtgatcccag aggctgcggt cctggtcggc gagcaacacg aggtcaccgt ccggggtgat
     1321 acgggtgcag cggcgcgact cgacgagcag catgagcgcg agcagcccca tgacctccgg
     1381 ttcgtcgggc atgagctcgg ccaggagccg gctgaggcgg atggcgtccg cacagagctc
     1441 ctcgcggacc aggcgctcgc ccgagcttgc cgtgtagccc tcgttgaaga tgaggtagac
     1501 gacggccagc acggctcgaa ggcggtccgg gaggtcggcc tcgttcggca cgcggtacgg
     1561 aatccgcgcg tcgcggatct ttcccttggc ccgcaccagc cgctgggcca tcgtcgactc
     1621 ggatacgagg aaagcgcgcg cgatctccgc ggtggtgagt ccgcccagga gccggagcgt
     1681 cagcgcgacc cggactccgg cgccgagtgc gggatgacag cacgtaaaga tcaggcgcag
     1741 tcggtcgtcg cgcactgtgc tctcctcggc tgtgcttgcc tgggccgggt ccccagcggt
     1801 ggatccgtcg gcccgggcgt gcaagagggc ggcctgggcg tgccggtcgt cgcgggaggc
     1861 ctcgcggcga aggcggtcga tcgcccggtt gcgggcggtg gtgatgatcc atcccgctgg
     1921 gctcggcggc agtccggtgg acgaccaccg tcgcaccgcc gcggtgaacg catcctggac
     1981 cgcctcctcg gcgacgtcga tgtcgccgaa gacgcggacg agcacggcca ccgcgcgtcc
     2041 gtactcctcg ctgaagacgc gttcgatctc ggaggtggga aggggcggca tgcgagggat
     2101 cgctcctcca ggacttcgtg accgtatggc cggcagtgtc ccggcttctc accgtctaca
     2161 cgaactgcca ctggccggtt cgacaaggcg cgcggcacgg cagcaggggg agtgtgttgc
     2221 gttgtccggc gcaccgcgag ccgcccggta ctgcgactga agagctgatg tggcggggtg
     2281 gagccgcgcg taaccttgcc cgctcacggt ccgtgccagc agcgatgtac ctgaccgccg
     2341 tccggcggcc gggcaggcga cgcccggctg tcgcagtgtg cagccaaggc aagggggctg
     2401 ttcacatgac cgtcgacgtc gtcactcgcg atggcacgca ggaacgcgtc tccgacgatg
     2461 ccatggcgga gctgacggcc cggttgaggg gcccgctcat caccgccgac gatcctgctg
     2521 ccgatgtcga gcccgtcccg gtctggaacg cgatgcacgt cgaccggccg gctgtgacgg
     2581 cccggtgcac gggaaccacg gacgtcgtgg aggcggtgac ctttgcccgc gagcacgggc
     2641 tgctgctggc ggtgcgcggc ggcgggcact cggtggccgg tttctccacg gtccgcgacg
     2701 gcatgctcgt cgacctgtcc tccatgcgcg gcgtgcaggt cgaccccggg cgtcggctgg
     2761 tccgggtcca gggcggtgcc ctgctcgccg acgtcgatcg ggagacgcag gcattcggcc
     2821 tggccgcccc gttgggtcgc gtgtccgaga cgggcgtggc cgggctgagc ctgggtggcg
     2881 gttacggcca cctccagaca cggctcgggt tgtcctgcga caacatcgtc gaggcgcagg
     2941 tggtgtgcgc cgacggccgg atccgcatcg cctcggcggg cagcaacccc gatctgttct
     3001 gggccatccg cgggggtggc ggcaacttcg ggatcgtcac gtcgttcacc ttccggctgc
     3061 accccgtggg gccggtcgtg gcgttcgccg ggacgttcta cccactcgag gatctcccgg
     3121 acgtcgagcg ccggtggcgg gaccatgtgg tgatcgcgcc cgatcaggtc acctcgttca
     3181 tcgtgacgat gaccttcccg gccgcgcccg agatgccgga ggtgatccac aatcggccgg
     3241 tcgcgatcgt cgggggcgtg tactgcggag atccggacga cgggatgcgg gtgatgcagc
     3301 cgctccgcga gttgggcacg ccgctgttcg acatgtccca gcccatgccc tacaccgcgg
     3361 tgcagtcgtc gttcgacccg ctcttccagc gcaggtcgct gcgcgcgtac tggaagtccc
     3421 agtacctcga cgagctcacc gacgacgcca tcgaggccat cgccgggcgc gcgctcgacc
     3481 ggcccgcgcc gctgtccatg gtcaacacct tccacatcgg cggcgccgtc catgctgtcg
     3541 gaccggagga gacctcgttc gccgagcgct ccgcgccctt catggtgtcg atcgacacca
     3601 actggtccga cccggcccag gacagcgccg cgatcgcctg gggacgggat gcgtgggagg
     3661 agatgaccaa atacggcaat ggcaacgtct tcctcaactt caccggcctc gctgacgagt
     3721 cggtacgggc gggggtggac agcgcgttcg gccgcaacct tcggcgcctc ggccagatca
     3781 aggcgaccta cgacccggac aacctgttcc aggtgaacaa caacatcatg ccggcccagt
     3841 agcgcccgcc gaccgttgct ggccgcgacg gctcgaccac actgtgacac aatagggggc
     3901 gccggcggag taggtgtgac ctatgttgcg aatgtttgat cgcgcgatcg agggcgctcg
     3961 tggcgccgac gggcggcggc cgaagaaacc gccgggtagc agcggggacg gcccaatgcc
     4021 cacaactgtg gaagtataaa cgctcatgtg atgagggtca cagtttgggc gttaatgcgg
     4081 caataattcc gccgtagcag cagcggccgt gttgcaccgc tgttaccttt cttcgacatg
     4141 gccgcacccg caacggactc acgttccgct ccccggactg tcgaacaggt gtcgctcggc
     4201 cgtccgcggg tggccgacgg agttgcctgc tggaacctcg cagcggcgtc ccgtgtgctc
     4261 gacgtgaact cgcgctacgc gtaccttctg tggttccggg acttcgcagc gacatcgatc
     4321 gtggcccggt tggacggcga gatcgccggg ttcgtcacgg gcttccggcg ccctgacgag
     4381 ccgcgcacgc tcgtcgtctg gcaggtggca gtagccgagt cggcccgcgg ctgcggagtt
     4441 gccggcgcga tgctcgacgc gctgttcgac gccgtgccgt acgtcaacca cctggagacc
     4501 acgatcacgc ccgacaacgg ggggtcaatc gctctgttca tcggattcgc gcgaaggcgc
     4561 ttcgcggaga tccgacgaac ggagctgttc ggctccgacc tcctcggcgc ggatcacgag
     4621 cccgagatcc ttttccggat cggtcctatt gatcgacgaa accattggga tgatgtgtga
     4681 tgaccgactt cgatgtggag tctcaggtcc gcagttactg ccggagttgg ccggtggtgt
     4741 tcgacactgc ccgcggtgca cgcctcaccg atgtcgacgg tcgctcgtac ctcgatttct
     4801 tcgcgggcgc gggcgctctc aactatggcc acaatccgcc ggcgctgaag gaaccattgc
     4861 tcgagtacct cggccgcgac ggcatcacgc acggtctcga catgtacacc gtggccaagg
     4921 gcgagttcct gcgcacgttc gaggagctcg tgctgcggcc acgcgggctc aactacaagg
     4981 tgcagttccc gggccccacc ggggccaaca ccgtggaatc ggcgctcaag ctcgcccgga
     5041 agatcgccgg gcgggagtcg atcatcaact tcacgcacgc cttccacggg atgactctgg
     5101 gcgcgctgtc ggtcacgggc aactcgatga agcggggcgg cgccggtatt ccgctcgtgc
     5161 acgccacgcc gatgcccttc gacaactact tcgacggcac ggtgccggac ttcctctggc
     5221 tggaccgcct cctgagtgac tccggcagtg gcctcaacga gcccgctggg gtcatcgtcg
     5281 agacggtgca gggagagggc ggcatcaacc ccgcccggat cgagtggctg cagggcctcg
     5341 ccgacctctg ccgacggcac cacatcctgc tgatcgtcga cgacgtgcag atgggcgtcg
     5401 ggcgcacggg ccccttcttc tccttcgaga tcgcggggat cgagcccgac atcgtctgcc
     5461 tctccaagtc gatcagcggc tatggcctgc cgatggcact cacgctgatc aagccggagt
     5521 acgaccagtg gggccccggc gagcacaacg gcacattccg cggcaacaac cctgcgttcg
     5581 tcacggcgac cgccgctctg cgcgagtact ggtccgacga cgcgctggag cgctccacgc
     5641 tggccaaggg cgagcgcgtc gaggcggcgc tcacggagct ggccgattcg tcccaggccg
     5701 tcgagctgac gcccaagggt cgcggcctgg cacggggtct gtccttctcc caccccggga
     5761 tggcggccaa gacgtgtgct gcggcattcg aacgaggact cctcatggag acggcagggc
     5821 cgtccgacga ggtcatgaag atcatgccgc cgctgacgat cagcaacgcg gaactcgagg
     5881 agggactggc agtggtgggc gatgcagttc gtaccgtcac cgggggggtg gttgcgtgat
     5941 cgtgcgcacg ctggacggaa tcaccgacac cgagcgcgac gtgcggaccg agcactggcg
     6001 cagcaagcgg ctcgtgctgg ccggcgacgg cgtcgggttc tcactgcacg agaccgtgct
     6061 cgatccgggc tcggtcaacg acttctggta tgccaaccac atcgaggccg tcttcgtgac
     6121 cgaaggcgaa ggcgagctct acgacaagga caacgacgtg acctaccgcc tggcgccggg
     6181 gtccgtctac gtcctcaacg gcaacgagca ccaccagttg cgtccgcgga cgcggatgcg
     6241 gacggtgtgc gtgttcaacc cgccggtaac gggccgcgag gtgcacgacg agaacggcgt
     6301 gtatccgctg atcatcctgt ccgcggagga ggaggcttcc tgatgaccgt gctcgacgca
     6361 cccacgaccg atcgctaccc cactcgaatt gccgaccggc ccgcactgat ccagcgcggc
     6421 gaacccaccg tctggggtgg gacggacgcg cccggtcccc tgtcgcggga cgagctcacc
     6481 gcacatgacc gtgacggctt cgtcactgtg ccggagctgc tgacccctgg cgaggtggcg
     6541 tcgtacgccg cggagctgga ccggctggcc gcggatcccg ggatccgcgc ggacgagcgc
     6601 acgatcgtcg agaagagctc gcagcaggtg cgctcgatct tcgaggtaca ccggatcagc
     6661 gaggccgtgg ccgcgctcgt cgccgacgag cgcgtggccg ggcgtgcgcg ccagatcctc
     6721 ggctccgacg tctacgtcca ccagagccgc atcaactaca agcccggctt cggcggtggt
     6781 ggtttctact ggcactcgga cttcgagacc tggcacgccg aagacggcat gccggtgccc
     6841 cgtgccgtga gcatctccat cgcgctcacc gagaactacc cgcacaacgg ctgcctgatg
     6901 atcatgcccg gctcgcaccg cacgttcgtc gcgtgcgtcg gcgagacccc cgccgaccac
     6961 tacaaggagt cactgcggga gcaggagatc ggcacacccg acccggacag cctcacgaca
     7021 ctggccgacc ggcacgggat cgcgatgctg acgggcccgg ccggctcggc cacgctgttc
     7081 gactccaact gcatgcacgg ctccgccagc aacatcacgc cgtactcgcg gtccaatatc
     7141 ttcgtggtgt tcaacagcgt ggagaacggc ctgaccgagc cgtacgcggc accgtcgccg
     7201 cggccgtcct acatcgcgac gcgcgagatc gcgccgatca cgggctgacg tcctacaggg
     7261 cgggttggcc gccgtcgagg gggagcagct ccgggcgctt gggggcgaat ccatcgcccg
     7321 tcttgtcacc ccgcagccgg cggcggacgt agggcatcac gaagtcgcgc atccagagca
     7381 ggtcctcgcg ctggcggtgc cgccacggcg agggctgtgc ctcgggcagt ggcgcgcgcc
     7441 agtcctcggc gacgggctcg ccgagcgtct ccagcacccg gagcgccacc cggcggtgcc
     7501 cctccgcggt gaggtgcaga cggtcactgc cccaggtgcg gggatccgcc agcgaggcca
     7561 tgccccagag gtcgacgacg gtgctgccgt ggcgttcggc gctcgcccgc atcagctcgt
     7621 tgtagcaggc cacccggcca cgcaggcggc ggatgatcgg gtgcatccgg cccaggtcga
     7681 agccggtgaa gacgaacacc tgcgcgccgg tggcggtgac ccgctccagc gcggagtcga
     7741 accggcgcgc gaggtcgtcg gtgtcgcagg tgaagcggat gatgtcgttg ccgcccgcac
     7801 agaacgcgat caggtcggga cggagccgtt cggccacggg gagctggtcg gcgacgatct
     7861 ggtcgagcag cttgccgcgc acggcgaggt tggcgtagcg gaacgccggc cggtttgcgc
     7921 ccaggcactc ggccacccgg tcggcccagc cgcgcggcga gccgtccggc cgccagtcgt
     7981 ccagaccctc ggtgaagctg tcaccgagcg ccacgaagct gttccaggcc acgtcgcgcc
     8041 ccttccgttt acgtccgctt cacccccttg acgggtgcag atcgctccaa tatcacccag
     8101 gaccagtgat ccgcgccagg ttccggccgg gtgatgtgcc ctcggatgac cacgcgaacc
     8161 acgcatccgg gctgtcatag ggcacccttg cctcgtggcc cgttgcaacc cgaagcccga
     8221 tgcgtccctg gccgagatcc tgggcggtcg cagcggcgcg ctggaagcaa ccgttccggt
     8281 cgttgcgttc gttgccgtct tcaccctcgt cgacgccgcg gcgtgggcct ggccggtggc
     8341 atgggcaggc ggggcggcgg tgcttgcggc cgcagcgatc ggcggggtgc ggctggccgg
     8401 tggtaggcgc ccgcgcgctg tgctgttcgg gctgctcggg gtggccgcag ccgctctggt
     8461 ggcgctctac accggccgcg cggtcgactt cttcctggtt cagatcgtgt ccaacgccgc
     8521 aagcgcgctg gcctgggcgg tgtcgatcgt gatcaggtgg ccgctcttgg gcctggtcgt
     8581 gggtacggtg ctcggccagc acagccggtg gcggcgcgac ccgcacctgc tgcgcggcta
     8641 ccagcgggcc agctgggtgt gggtcggcca gtacctcgtg cgggtggcgg tgttcctgcc
     8701 gttgtacttc gccgatgccg tgttcgccct gggcgtcgcg cggtcggtgc tgacctggcc
     8761 gctcgtcgcc ctgagtgtcg ccttgtcctg gccgctgatc cggtccgcgc tgccgccggg
     8821 acaccgcggg ctgcgccatc cggcggaggg gtcgccggga tcccgctcct cgacgtgagg
     8881 gcggtcacga acggtgatca tctggagaga tccacggctg cggggcgttg acccaggtgg
     8941 acctcgacaa cacatacatg tgatcgccga caggagccat gccccaattc cccacagccc
     9001 cccgccctga ctcgtcccgg accggcccac gccggacccc gccgccccgc atcccgccgc
     9061 cccggatccc gccgctcccc ccgacgcggg tctccccgtc cgcgaacgag acggcggtgc
     9121 aggcgcggcc gaagggcggc ctggacctca cggtcaacaa ggtgatcgcc ggagccggtg
     9181 ccgcggccac gagcgcggtg ctcgggtcct tcttcggagc agcgggcacg gtgacaggcg
     9241 ccgcactggg gtcggtggcg agcaccgtgg cctcgacggt ctaccagcgt tcgctggacc
     9301 gcacccggga caggctggtg acgcggatcc ggctcaccgg ccggcacggc gcccagggag
     9361 cgcaggacca ggccacccag gtcatgatgc cggtgccgcg tgtctcaccg gagggggtca
     9421 cggcgcgggt ccgcgtcgaa cccgccgttc cggcgcggcc ctcgaagcgg cggttcgtcc
     9481 tgtggtcggc ggcgaccgtg ctcgtgttcg tgctcggcct gctcgccgtc accgggatcg
     9541 agtgggccaa ggggtccacg ctcacctcgg gggagtccgg cacgtccgtc gggcgggtga
     9601 tcgaggggaa ccacagcagc cggcacaggg tcgagccggt cgatccgacc ccgaccacga
     9661 cgccggaacc gtcgagcact ccggacccca gcgccgatcc cagcaccagt tcgagcgccg
     9721 agccgagcgt cggcccgact ccgtcgaacg gtcgtgactc cgggagcccc acctccgcac
     9781 ccgccgcccc cggcgccgga tcgggaggta acgacggttc cgggaacggt ggttccggga
     9841 acggtggctc gggcaacgac ggctcgggca acggtggctc gggcaacgac ggctccggcg
     9901 ctacgggcga cccgggcagc ggctccggcg gtcagggcgg cggctcggtg cccaccccac
     9961 tcgtgccggt cccgggcggg cccgggaact gaggttccgg cagccgacgc accggctgcg
    10021 gatgcttgca cctgacccgc aaccgggtgg gtggtccgtg cgaccggtgt gtgggcagga
    10081 tcagccgctc agccgctcag ccgctcagcc gctcagccgc tcagccgctc agccgctcag
    10141 ccgctcagcc gcggagccgg gcggccaagg tcgcggcggc cgcgcgaggg tcgtcggagt
    10201 ccgtgatcgc ccgcaccacg acgatccggt cggcgcccgc gccgagcacc tcgtcgagcc
    10261 ggtcgtggtc gatgccgccg atggcgaacc agggccgcga cggcgaacgg ccggcaacgg
    10321 tgcgcacgag atcgagtccg ggcgccggac ggcccggctt ggtcggtgtc ggccagcagg
    10381 ggccgacgca gaagtagtcg acgcccgcct ccgccgcggc ggttgcggtc tcctcggcgc
    10441 tgtgcgacga gcgcccgacc accacatcgt cgccgacaat cttgcgcgcc cactcgatgg
    10501 gcaggtcgtc ctggccgagg tgcaggacgt cggccccggc ggccaccgca acgtcggccc
    10561 ggtcgttcac cgcgagcaga gctccgtgcc gggcgcacgc ctcggcaagc acctccaggg
    10621 cggcgagctc ctcgcgggcc tccagcgcgc cgtcggcacc cttgtcgcgc aactggacga
    10681 tgtcgacccc gcccgcgagc gccgcttcgg cgaactcggc gaggtcgcca gtgcgtcgcc
    10741 gggcgtcggt gcagaggtag aggcgggcgt ctgccagtcg cgcgcgaagc gcgtctccgt
    10801 ccagtccggg cacggtgacc gaccctacgc ggcgcgcacc gcggcgcgta ctgtgaaccg
    10861 caggtgacgc acgggagccc ggccagcggg ctgagagggg atccagcgat cccgaccgtc
    10921 gaacctgatc cgggtcatgc cggcgcaggg agcggggaga tgaagaattc acggttgacg
    10981 gtgatcgggg caggcgtgat cggcctgtcc tgcgcctggc gcgctgccgc cgcggggctg
    11041 cgggtcacgg tgctcgaccc cgcgccggcg tccggggcgt cctgggtggc aggcggcatg
    11101 ctcgcgccgg tcaccgaggc gtggcccggc gaggaggcac tgctggagct cggcgtcgag
    11161 tccgtccggc gctggcgggg cttcgccgag gaggtcggcg ccgccgcggg ccgcccggcc
    11221 gggctgcgga ccgagggcac ggtggtcgta gccaccggcg cgggcgaccg cgccgagctg
    11281 gacacgctgg cgggctatct cgcgcggctc ggccgcgatg tcgagcgcct ctccggccgc
    11341 gagctgcgcc gcctggagcc cgcactgggc ccggacgtcc gcggtggcct gtccgtgccc
    11401 gacgacctgg cggtggacaa ccgtgtgctg ctcgcggcgc tgcgggtcgc gggcgagggt
    11461 gccggcgtca cgttcgtgga gcgcgccgca cgggccgtac tcgacgacgg aacgcgcgtg
    11521 acgggcgtgc gcaccgatgg cgccgacctg gcgtccgatg tcgtgctcat ctgcgcaggc
    11581 gcccactccg gctcgctgca cccggtactc cacgggctgg tgcgtccggt caagggcgag
    11641 atcctgcggc tggcgcaccg cagcggggcg ttcccgccgc ccacccgtac cgtgcgggca
    11701 ctggtggacg gccgtcccgt ctacgccgta ccccgcgacg gaggcgggct cgtcatcggc
    11761 gccacgcagg ccgagaccgg tttcgacacc gaggtcaccg tcggcggcgt gcgcgacctg
    11821 ctgcgtgacg cggagcgggt gctccccggc atcgccgagt acgcgctggt ggagagcgcg
    11881 gcagggctgc ggcccggcag cctggacaac ctgccgctga tcggcgcact cggcccggac
    11941 gggctgctcg tcgccaccgg ccaccaccgc aacgggatgc tgctcgcgcc ggtcaccgcc
    12001 gacgcagtgc tggagctcct gcacggccgt ccggttcccg aagcggtgcg cgcggccgac
    12061 ccggatcgat gcgtcagcgg agcgaaccgg caccacgagg aggtagcacg tgcacgtgtg
    12121 gatcaacggt gagcagcgtg agctggaggc cggcgcgagc gtgctcgacg cgctgaccgc
    12181 gctgggcgcc ccgagcagcg gcgtggccgt cgcggtggac ggggaggtcg tcccccgggc
    12241 cggctggtcc ggcaccggcc tggtcgacgg cgcgcggatc gaggttctga cggcggtgca
    12301 gggaggatga cgatgaccat cgataccggg ctcctggtga tcggcgaccg gaagatcgca
    12361 tctcggctga tcatgggtac gggcggggca gcgaacctgg agatcctgga gcgcgcgctc
    12421 gtcgcgtccg gtacctccct gaccacggtg gccatgcggc gcatcgacgc cgcgaccggc
    12481 acgggggtgc tcgacctgct gcgccgcctg gacatcgagg tgttgcccaa cacggcgggc
    12541 tgccgcaccg ccgcggaggc cgtgctcacc gcgcggttgg cgcgcgaggc gctggagacc
    12601 gacctcgtca agctcgaggt cgtggccgac gaacgcacgt tgctgcccga cccgatcgag
    12661 ttgctggacg ccgctgagca gctcgtcgac gacgggttca cggtgctgcc ctacaccaat
    12721 gacgaccccg tgctggcccg caagctggaa caggttggct gcgcggccgt catgccgctg
    12781 ggttcgccga tcggcaccgg gctcggcatc cgcaacccgc acaacatcga gatgatcgtg
    12841 gctgcggcgt cggtcccggt cgtgctcgac gcagggatcg gcacggcctc ggaggccgcg
    12901 caggcgatgg agctgggctg cgacgccgtg ctgctcgcca cggccgtcac gcgggcgggc
    12961 gacccggagc ggatggcgca cgcgatgcgg ctggccgtgg aggccggtcg ggccgcccgc
    13021 ggcgcaggcc gcatcccgcg gcgctactgg gcccaggcct cgtcgccgga ccccgacgcg
    13081 tcgtagcgca ggcagtcacc ccccgcgggg gcatcagcag tcgaacgccg ggacgaccgg
    13141 ggtgactccg gcgcgatggc gcctggcgag ctcctggtag atgcccgggt tgccctcgac
    13201 ccaccagcgg gccgactccg tcagggggcc cttcacacgg gcgaccgtgc ccagcacgag
    13261 ctggccgtcg ggcaccacgg tgttcggcgc gaccgtggcc cccgcgccca cgagacagcg
    13321 ggctccgatg cgggcgccgt cctggatcgt cgaaccgttc ccgatcagcg cttcgggacc
    13381 gatcacgcac ccgtgcacca cgcacaggtg cccgatcgtc gcgcccgcac cgacctccgt
    13441 gatggggtcg tctccgccgt gcagcacaca gccgtcctgg acgttcgccc cttcgcggat
    13501 gatgatcggt ccgaagtcgg cccgaagcac cgcgttgtac cagatggatg cgtgcgcctc
    13561 gacgcgcacg tcgccgacga gtgtcgcggt gggggcgatg aacgcgtccg ggtgcacggt
    13621 cggggacagg ccctcgaagg agaacaacgg catgggccgc agggtgccac ccgggtgctt
    13681 ccggcgcgtc gcccggtcgg cggcccggga ccaaggcacc gtggagggct gcaggcgttc
    13741 ctgcggaacg gggcgcgctg tgtggaggtt gtcggggtga tcgggcgacg gggcacgcca
    13801 ctggtcctcg ctgtgctgct cgccgggtgc ggcagcgcac ccctctcgtc gagccagccg
    13861 tcagcactcg cgacgcccgt gtcgagcctc ccgtccagcc cgccggcagg ctcagcgggc
    13921 gaagcggcgg cactgccgat ctactacgtg gccgagaccc cggccggctt ccggttgctg
    13981 cgtgagttcc atcgcgtccc gtctgccgac ccgggtagcg acgcggcccg cgagatgctc
    14041 gcgaacccca ccgggaccga ccccgactac cgctcgcact ggccgcccgg cacgaccttg
    14101 cgtacccccg tgcgaaccga cggcgggacg atcgtggtcg atctgtcggg cgctgacgct
    14161 gcgcgcgtcg atgccgagct cgcggagccc accgtgcagc agctggtgtt cacggtccag
    14221 ggagcgctgc agtccaccgc cccggtgcgg atcctggtgg acggcgagcc ggtggaccag
    14281 ctgtggggag ccgtgtccac ggccgagccg gtggagcgtg gcgacgacta cgcgctccgc
    14341 tcgctggtcc agatcgacgc tccggcacac ggcgcaacgg tcggccgcga ggtcgaggtg
    14401 aggggcgagg ccgcggtgtt cgaggccact gtcgggtggg aggtgctgcg cgacggcgca
    14461 gttgtgcggt cgggcttcac gagcacgacg gagggccagc ggttcgcccc gttcgcattc
    14521 gcggtgacgc tggagccggg ggagtacgtg gtgcgggtgc tggaggacga cccgtcgggc
    14581 ggccagggac gcccggtcct cactgacgac aaggcggtga cggtgcgccc ctgagggccg
    14641 ctcagggctc cggacacctg cctcagtcgg gtggtcgcgc aggggtccgc aggcccaggc
    14701 tccgcagctc cagcgccgcg agcccgtgca ccacggaccg gtcgtcacgg cgccatgcgg
    14761 ccgccgggtc gggggacacg gcgagcagcc gccggaccgg cagccgggcc atggcccgca
    14821 gggcgagcag atcggagtcg acggaacgca cggcgatggc cgaccgtgcc gcccgcgcgt
    14881 agcggacgcg catcggcagc cacaccagca gcaccggcat cgccgccagc agcacgatcg
    14941 cgatcgccgc gccggacgcg acggtcgcga ccgtctccac ctgctcccgc ccggccgcgg
    15001 ccagcgactc gcccgccccg gtgccggtcc ccagcgcacg agcgagatcg tccccgacga
    15061 acggcaccgg ctgcgcggtc cgcgccgcgt catcgaaagc gccgcggatc gcgtcgcccg
    15121 ccccgatcag actccgtccg gggccctgca ggcccaggat caggtcgcgc gcagtacctg
    15181 cgaccagcac ccacaggacc agccagccga tggccagcag gtcgaccagg agctggagcg
    15241 ccacccgagc ggggcgttcg gcgtagaacc gcatggcggg cgagcgtacg gatgggccgc
    15301 agccgatcgg tcgcccggtt cctgtccgcg agtcgcccgg ttcacgtccg cgagtcccct
    15361 gcctcgacag cgcggcggcg gaggcggcgt cccaggcctt gggcggtcac acgtgaatcg
    15421 ggccctcgcg ctcggcgagc cgggcgccgg caccgcccca gtgcagggcg atcatctccg
    15481 ccgcgatcga caccgccgtc tcctccggcg tgcgggcacc gaggtccagc ccgatcgggg
    15541 acgacatgcg gccgatctcg gcctcggtca cgcctgcctc gcgcagccgg gccaaccggt
    15601 cctcgtgggt gcgccgcgag cccatcgcgc cgatgtagcc gacctccgga agccgcaggg
    15661 ccacctcgag cagcgggaca tcgaacttcg gatcgtgggt gagcacgcac agcacacttc
    15721 gggcgtcgat gcgcccggcc tccgcctcag cggcgaggta gcggtgcggc cactcgacga
    15781 cgacctcgtt ggcaccgggg aatcgggagg cggtggcgaa gacgggacgg gcgtcgcaca
    15841 cggtgacccg gaatcccagg aagctgccga tcttcgcgac cgccgccgcg aagtcgatcg
    15901 ccccgaacac gatcatgcgg ggcggcggtg cgaacgactc gacgaacacg tcgaggccct
    15961 cgccgcggcg ctgcccgtcc acgccgtagt gcagcgtcgc gttgcgccct gcgtcgagca
    16021 ggccacgggc gtcgtcccgc accgcgtcgt cgagccgggc ggagcccgtg ccgccttcgg
    16081 tgcggtccgg ccagacgacg agccgcttgc cgaggcgctc ggcaggcccc gagaccacgg
    16141 tggccaccgc gaccggcgac tccgagcgga tcgcctcggc cacggcgccc agctggccgt
    16201 agctgtcctg atcgatcttc tccacgaaga tgtcgatgat tccgccgcag gtcagcccga
    16261 ccgcgaaggc gtcgtcgtcg gacacaccgt atcgctgcag caccggcctg ccatcggtca
    16321 gcacctggtg ccctagctcg tagacggcac cttcgacgca gccgccggag acgctaccga
    16381 cggcctcgcc ccctggtccg acgagcatcg aggccccggg ctgccgcgga gcggagctgt
    16441 aggtccccac gaccgtcgcg agcgcggcgg tctcgccctt gttccaccag ccctcgagct
    16501 ggcccactac gtcacgcatc tcgtaccacc tcgagcagtc gttccagcgt tgccagactg
    16561 tgacctgcca acaagtcgtc caagtacggc agcgcggcga ctattccgcc ctgcacgggc
    16621 gcgtagccct ccttccccac gtgcgggttc acccacacca ggcggtgcgc gagcctgcgc
    16681 aggcgggcca tctgctgccc gagaaggtcg gtcgagccgc gctcccagcc gtcggagaag
    16741 accacgacga cggcgcgccg ggccgcgccc cgttgccccc agcggtcgac gaacgcctgg
    16801 agtacctcgc ccagtcgggt gccgccggac cagtcgggta tcgccttggc ggccgcgccc
    16861 agcgcgcgtt cggcgtcacg catccggagc tcccgggtga cccgggtgag ccgggtaccg
    16921 agggtgaacg cctccacggc cccgggcgag cggcgcacga cgacgtgggc gaagcggagc
    16981 aatgcgtcgg cgtagggctc catcgagccg gagacgtcga cgagcagcac gatcttgcgc
    17041 ggccggcgcg agtggtcgcg ccgccgcagt tcgcgtagct caccctggtt gcgcagggcg
    17101 gcccgcagcg tcgcaccagg gtcggtgcca ccgtgccgtg ccggccgcat cctgcgcgac
    17161 acgcgggcgg gcagctcggg gtgcaggagg gcgagcagcc tgcgcaggtg ctcgcgctcg
    17221 gcggggctga gctcggcgag atcgcggttg cgcagcacct ccgtgccgct ggcccgcgcc
    17281 cggatcagtg ggtcctcccg gccgtcgtcg tcgctctcgc cgtcctcggc cgacggggtg
    17341 agcgcggcca gcttcggcgg cggcggacgg ttgtcggtga tctgcgtgcg cccgccggac
    17401 ggcggtgtga accagtcctc gaaggcgcgg tcgtagcgcg gcaggtcgtc ggggtcggag
    17461 cagagcgtga gccggccggc ccagtaggtc tgcacccggc tggtgacgtc gagtgcgtcc
    17521 agcgcggcga ggaatgtggc cacgcggtcc gtggtgatcg ccagtcccgc gttgcggagc
    17581 acgacagtga agcccaccag acccgggacc gggtcatcgg tcggcgccgg cgtggacgtg
    17641 gtcatgacac ctcaggaggc gagcagggtg tcgagctgct tacggacgcg gtcggcgtcc
    17701 tcccggtact tgaggaccgc accgagcgtg gcggcggcgc tgtcgacatc caggtgccgg
    17761 gcgccgacga gctgcagggc gcgggcccag tcgatcgtct ccgcgactcc cggcggcttg
    17821 agcaggtcgg cccggcgcag ctgctggacc gcacccgcca cctgcgcggc cagccgttcg
    17881 ggcaacccgg gcagccgcga gtgcaggatc tcgatctcgc gctgcaggtc ggggtggtcg
    17941 agccagaggt agaggcagcg gcgcttgagg gcgtcgtgca cctcgcgggt gcggttggag
    18001 gtgagcacga ccagcggtgg caccttcgct ctgatctcac cgacctcggg gatcgtgacg
    18061 gcgtgctcgg tcagcacctc gagcaggaat gcctcgaact cgtcgtcggc gcggtcgatc
    18121 tcgtcgatca ggagcacgct cggactggac tgcagtgcgc gcaggatcgg ccgggccagc
    18181 aggaagcgcg ggtcgtagag ggaggactcc acactgtcgg cgtccagcct ctcggcactt
    18241 gcccctgccg cggcctcgag cgcgcgcagg tgcagcagct ggcgcgggaa gtcccagtcg
    18301 tagagagcct gcgacgcgtc gatgccgtcg tggcactgca gccggatgag ctcggcaccg
    18361 aacacctccg cgagcgcatg cgccagcgcc gtcttgccgg tgcccggttc gccctcgcag
    18421 aacagcgggc ggttcatctg gatggcgaga aaggtggcag tggccacgcc gtcgtcggcg
    18481 aggtagccgg tggagcgcag cgcagccgcc agctcgacgg gcgaacccgc gatgttcggg
    18541 cgatcggtca cgcatccagc atgactgaac gacgtccccg cagccaccgc gggtgaggcc
    18601 ccacacgggg ctccgtggag cgcatgtcac gcgctgtgac cagtccatct actgccgaac
    18661 tgcgaatact tccactttga ttgcaagtta cgtctcgtga tgcggacgtt atccgtcggg
    18721 cggctgacct gcggatattc gaggagcggt ggttgcgtcc gcggcctaac gtgatgtcgg
    18781 tcacccatcc gggatttgac gtattcccgc ccaggactcg tagcgtctac ggccggtcgt
    18841 ccgagaggac cgatcggaat acatccggat ccgtcgcgcc aggccctgtc ccgcggttcc
    18901 ggtccgtcga gatggagagc gggacagggc cgaggaagct atttcgtcga agtcgcctta
    18961 ggacgtcgac gggatggcag gagctgttcg ccgggtcccc tcccaacccg ttgacgagca
    19021 ggttctcttc gaccgtgcga atggagaaca acgaaatgac ttctcgaccg gattcgcgta
    19081 agggtcataa gctgctgcgt ctcgctgtgg ccggagcagt tgtggttggt gtcccgcttg
    19141 ctgttgccgg caccgcgaac gccgcccccg actccgcttg ggacaggctg gctcaatgtg
    19201 agagtggcgg caactggaac atcaacaccg gaaacggttt ctccggcggt ctgcagttca
    19261 ctcccagtac ctggcgtgcc ttcgggggga ccggttcggc caaccaggcc agccgtgagc
    19321 agcagattgc cgtggccgag cgggtgctgg caacccaggg ctggaacgcg tggcccgtct
    19381 gctcggtcaa gacgggtgtg cgcggaaaca ctgccgaggt gcgggtctcc gcgccggttg
    19441 cgaccaggat cgaggctccc gccaaggcgg aggtcaagaa caaggtgcag gccccggcca
    19501 aggcgaggac cgctgccccg cggctcacca agaacggtgc gaactacacc gtcgtcaagg
    19561 gcgacacgct cagcaagatc gcggccgagc gacgcctgcc gggcggctgg caggccatct
    19621 accagcgcaa cacggacgtc ttgagcaacc cgaacgtcct gagcatcgga cagcagctcg
    19681 acctcgcctg acgcacccgt gcggggtacg ggccaccagc gcccgtaccc gcccggcgtc
    19741 gggacgctcg cgggatcatc aggtcgagcc gacccactcc tcggtgccgt cggtgaagac
    19801 ctggtgcttc cagacgggca gcaggcgctt cacctcctcc accagctcgg cgcaggtctc
    19861 gaaagcctcg cgtcggtggt cggccgcaac ggcgcaggcc agcgcgacgt cgccgatcgc
    19921 gagccgtccg acccgatggc tcacggcgat cgcccggacg ccccgggcgt gcgcggcgat
    19981 ctcggcggca acctcggcga cgaccttctc cgcggtcggg tgagcgctgt actcgagccc
    20041 ttgcacggaa cggccgccgt cgtggtcgcg caccacaccg gcgaacgtca cgaccgcgcc
    20101 tgccgccgcc tggtcgacga gcgcggcatg ctcggccacg tcgagcggcg cgtcgccgac
    20161 agcggcccgc agaactgccg ctccgttcgt cacgtcggtc atcgatgatc gcctcctcga
    20221 agctggtcca ccgcgtgctc cagcacatcg gcgaggaccg acagaccgtc ccgaacgcca
    20281 cctgtcgatc cgggcaggtt caccacgagt gtgcggcccg caacgcccgt gaggccgcgc
    20341 gacagcaccg cggtcggcac cgtcggcgcc ccggcggccc gaatggcgtc ggccaggccg
    20401 ggcacctcgt agtcgaggac ggcccgggtg atctccgggg tcgcgtcggt gggcgagatc
    20461 ccggtgccgc cggtggtgat cacgacgtcg acccggtccg cgacggctgc ccgcagtgca
    20521 tcccccaccg gctcaccgtc gggcaccacg gccgggtccg gggtctcgaa ccctcgctcg
    20581 cgcagccagt cgacgatgag cggcccgcca cggtcggcgt agacgcccgc tgcagcccgg
    20641 ttcgacgcag tcaccacacg ggcgcgccgg tctgcggggc tcacgaccgg tcctccgggc
    20701 gcgtccaggt gccggtcttg ccgccgtcct tgcgctccac gcgcaccccg tcgagcacag
    20761 cggcggggtc gacagccttg atcatgtcgt gcagggcgag cccggcgacg gcgacggcgg
    20821 tgagtgcctc catctcgacg ccggtgcgat cggtggtgcg caccgtggcc cggatccgta
    20881 cctcggcgcc gcccggctcg aggtccagta cgacgccgct cagcgcgatg gggtggcaga
    20941 gcggcacgag gtcgggggtg cgcttggcgc ccatgatccc ggcgatccgg gcggtggcca
    21001 gtgcgtcgcc cttcggtagg ccgtcgcggc gcagcagctc gatcacctcg gcggtggtgc
    21061 gcagcgttcc ggtggccacc gcggtgcgcg cggtgggctc tttgtcggtc acgtccacca
    21121 tgcgggcggc accggcggca tcgacgtggg tcagctccac actcgaaccc tacggcgtga
    21181 tcggaggcga tgctcggggc gcgaagcgtg cgcccaaatg ggtgatctcc atcacgccga
    21241 cgcaacctct ggcagtaccg agcgttgtgg tcaacgtaat gctgagcagg cacgatcctg
    21301 ccgtgttggg ccattaggtg acacggttgg gacgaaatgc ttccaatgtg gtgggttttt
    21361 tccccgccct tccggacgta gcgtcctccg cggctcgacc ggaacgcgct ccccaggtcc
    21421 gttcgagccc cggaaccgca gcgctcccct gtccggcggt cccggccccc gttgcctcac
    21481 gaagcgcggg acaggaacgg actatggcgg ccccggccgc cccgccagct ccccggcggg
    21541 tccaggcgca gcgcacggag atacgtcatg cgctaccgcg gccgtcaccg tgcaccgacc
    21601 tccaccgggc ggacgatcgc ccgcaccgct ctcgccggag ctgtcgccgg cgctccgctc
    21661 gtgatcgccg cgcctgcggc gcacgcggct tccgacagca cctgggaccg cgtcgcgcag
    21721 tgcgagagcg gtggccggtg ggacatcaac accggcaacg gctttcacgg cgggttgcag
    21781 ttcacgccga gcacgtggaa gggcttcggc ggtaccgagt tcgctcccgt cgcatatcag
    21841 gcgagccggg cccagcagat cgtcgtcgcc gagcgcgtgc tggccaagca gggctggggc
    21901 gcgtggccgg tgtgctcccg gaaggccggc gccgtcggag aacctccgtc ccagcgcagt
    21961 gcgccggcca cgcaggtccg gctcagcgcg cccacccccg ctgccaccag cgggtcctac
    22021 gtggtgaagc gcggggacac cctctccacg atcgccgccc agcacggggt cgccgacggc
    22081 tggaagggcc tggtgcagaa gaaccccggg ctcgccggca atccgaacag gatcagcccc
    22141 ggccagcaca tcgcggtctg aacactcccc ggccgggccg cagcacaccg aaccccggac
    22201 gtgggtcagc gggaccggaa gcgggccagt tgctcggggg tgtcgacgtc gtcggggtct
    22261 gccaggtccc cgcactccac gatggcgaga tccgggtgcc cggccaggta cgcgcgggcg
    22321 ccggcatcgc cgatggccgt ggcccggacg cccgaccagt gtgaccgtcc gatcaggacg
    22381 ggatgggccg gcttcccgtc gtacgctgca cgcaacagca cgtcgggcgc gctgccggcc
    22441 ccgagccgcg caaccgccgc cgccgtgacg cccggcaggt cgaccaggtg gatgagcgcg
    22501 gcgtccggga ccggggagag ggcctccagc gcggcgagcc ccgcgcggag ggaggccccc
    22561 attccctcgg cccagtcctc ggccacgatc acggttacgc cggacgggac gatcccggcc
    22621 acctccgcgg ccgcagcgcc gagcaccacg acgagcggct cacagccgcc ggcggcgagc
    22681 acccgcaggg cacgccggac gagcggctcg ccgtccagct cgacgagcgc cttgggaccg
    22741 cccatccggc gtccggcgcc ggcggcgagc agcagaccgg tcggggcgcc tacttgttta
    22801 tctccgtgac agggtgcacg tagggcacct cctcgagcgg gaactccact tcgccgaagg
    22861 gcgacagcga gccctgcaat gggctggcga gctcgctcac cggatattcg ccgtcgggca
    22921 gctccggcca cgacgggtcg atccggccct gctgggcggt cttggccaca gtgtcctcct
    22981 cggggtcggg ctttcgaacg attattccgt accggacccg gcgccgaggg acgaggtgcc
    23041 atgcccacct ccggcgtcgg tgcggcctac atgccgccgc gcgggcagtt gcggtcggcg
    23101 atacgaccgg tgtcctcgcg gcagtcccga ctaccgtgga cctgatgccc actccgctgg
    23161 tcgactggtt gcgcgcccag gatgacgaga cgctcgcggt cctgcttcgg ctgcgtccgg
    23221 atctcgccgt gccgcccccc gcggacctca ccgtgctggc cacccgagcc ggtatccggg
    23281 cgtccgtgca ccgcgcctgc gacgacctcg acaggatcac gctcgcggtt ctcgaggccc
    23341 ttgtcgtcgc cggcgccgac tccgcacctg tgccgctcga cgaggtccgc cgcctgctcg
    23401 gtcccgacgt ttcggtcacg gcactcagcg gcccgctcac ggcgttgcgc gaccgggctc
    23461 tggcctgggg acctgacggc gagctgtccc tcgttcccgc cgtgcacgag gtggtgccgc
    23521 gccaccccgg cggtctcgga cgtgaggcgg ccggtccggc agggtcgtcg gggctgcccg
    23581 agctgctggc cgccctgcaa cccgacgaac gccgggtgct cgaggcgctc gccgccggac
    23641 cgccgatcgg ccgcagtcgc gcggggtcgg atccggacag cccggtcggc aggttgctcg
    23701 cccgtgggct actcctgcgc gtggacgccg agacggtcga gctgcctcgg caggtggggc
    23761 tcgcgctgcg tggggagcgc ccgctcggcg aggtggccat taccccgccc gacatcgggg
    23821 cgcgagaccg cggagcacac tccgtcgacc gcaccgcggg cggcgcggcg ctcggggtgc
    23881 ttcgccagct cgagctgctc atcgcgttct ggggcagaac tccgccgccg gtgctgcgct
    23941 ccggggggct gggtgtgcgg gagctgcgca gggcggccaa ggagatggac gccgacgaga
    24001 cggtggctgc gctgctcatg gagctggcgg tggccgccga cctggtgggg gagagcgacg
    24061 gcgtggcgcc ggaatgggtg cctaccacca acgccgactt gtgggcggcg ggcggccccg
    24121 agctgcgctg gtcgctgatc gcgcgcacct ggctgga
//
LOCUS       DASRSC010000360         8378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4579040,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000360 DASRSC010000000
VERSION     DASRSC010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8378
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4579040"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..379
                     /locus_tag="VFE39_17455"
     CDS             95..379
                     /locus_tag="VFE39_17455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260426.1"
                     /translation="MAIYQMIGVRNELLFASALMSEPADQPIQPAATALIGRDSTNWA
                     ALTSAMTLSALPGDRVRGLPALVLRRAHRRRGEVLTGDIMIGQEQTSWTC"
     gene            367..600
                     /locus_tag="VFE39_17460"
     CDS             367..600
                     /locus_tag="VFE39_17460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260427.1"
                     /translation="MDVLTPQPLGPTGLKVTPLCVGSGELGDMTSVHGYAVPEEQALA
                     TVRAFAGPFTFVDTSNYGAGTSERRIGAVARWR"
     gene            591..836
                     /locus_tag="VFE39_17465"
     CDS             591..836
                     /locus_tag="VFE39_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260428.1"
                     /translation="MEVMQQLVAEGLVDNIGVAGGPVDLLRKFVATGAFEVVLTYNRW
                     TLLHRSAEPIRLATHPVPDELWTVLADLTPAEENWLR"
     gene            833..1930
                     /locus_tag="VFE39_17470"
     CDS             833..1930
                     /locus_tag="VFE39_17470"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET6260429.1"
                     /translation="MTAPAPTPRTPTTFGVVGSGWRSLFFLRLAQAAPDHLRAAGVVT
                     RTAERGQEVTDAWGVPTFRTVEELLAADRPDFVIAAVPWGQMPVTTREVVALGVPVLA
                     ETPPAPDLDGLRSLWSDVGGSGLVQVAEQYLLMPGHAARLAVLRDGLIGEPTSVQLSS
                     THMYHAVSLIRGLLGVDMDEAVVTGREFTAPLVDPLSSDGWKQDAVPEARTTTIATID
                     FGGRSGLYDFTDNQWWNPLRARRILVRGSMGELVDDRLVRMVDPTSPVESHLVYRRTG
                     VDMNLEGNDLDFVSVDGRVVYRNPFSGTRLSEDDIAVAAFLEATGAWARGEGPEPYPL
                     AQGCQDHAIALAIGESARTGHDVRVTKEPWA"
     gene            2031..3140
                     /locus_tag="VFE39_17475"
     CDS             2031..3140
                     /locus_tag="VFE39_17475"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HET6260430.1"
                     /translation="MRAAVLREPGTPLQVEDLEVDAPHAGEVLVRVEAAGVCHTELHY
                     MSGDIPCPLPIVLGHEGCGVVEHIGSGVDTVAVGDRVAFTWRPRCGFCEFCMTGRPVM
                     CIYGRVQARSGGLMDGTSRLRRGEEKVHHFLGVSCFAERAVVSQRAVVPLPEDVPVAV
                     AAVTGCAVITGLGAVVNVGGQLAGRPLLVIGAGGVGLSAVMGAVLVGAGPVVVVDVAA
                     DKLELARRLGATHTVDAGILDAAGVVESVQELTGGGAEVAVDAVGSPQTLRQAFTALR
                     PGGTAIAVGLAAVDATAAIPINELVQQQKHLVGSLYGSANPPVDLPRILSLYQSGRLP
                     LEALLGAQHPLEGVEDAYRALRAGAPGRAVVVPGP"
     gene            complement(3416..4072)
                     /locus_tag="VFE39_17480"
     CDS             complement(3416..4072)
                     /locus_tag="VFE39_17480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6260431.1"
                     /translation="MSSQIPAAAEAGLSGRRRQAAQNDTAILDAARAVFLDDPKAPIA
                     AVAQRAGVGISALYRRYPGKEDLLRTLCHDGLRRYLAEAEAAAADPDAWSAFSTFLQR
                     IVDADVHSLTVHLAGTFVPTAEMQRDAERAGNLGIALFERARAAGRLRPDVVGQDVGL
                     ALEACAAIRVPDPGRTRELRRRVLATHLAGLAADGDPRPPLPGPPPQAAELNWRWEQR
                     "
     gene            4153..5298
                     /locus_tag="VFE39_17485"
     CDS             4153..5298
                     /locus_tag="VFE39_17485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HET6260432.1"
                     /translation="MSNTDIRPFRVDIPQDALDDLDARLRGARWPHELPGSADVYGVP
                     VARVRELVAHWLDGFDWRAVEARLNAYPQFVTEIDGEDIHFLHVRSPRADATPVILTH
                     GWPGTVVEFLDVIGPLTEPASADDPAFHLVIPSLPGFGFSGPTRSGGWGTRRTARAWV
                     ELMSRLGYDRYGAIGNDAGSMISPEIGRIDGEHVVGVHVTQFFSFPSGDPAEFEKLTE
                     ADHAALAHLQSFLDTKFAFNVLHSQQPQTLAFALADSPVGLLAWNAQLMGESLDPDFV
                     LANVAIYWLTGTAASAIRFYYEDAHLAEEPAAEPTVAPTGLAMFAGDFQSIRAFAGRD
                     HANIVSWNSYDPGLPAGGRQDAPGHYAAHEATDLLVGDIRRFFAQLG"
     gene            5425..6450
                     /gene="cofD"
                     /locus_tag="VFE39_17490"
     CDS             5425..6450
                     /gene="cofD"
                     /locus_tag="VFE39_17490"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HET6260433.1"
                     /translation="MKVTVLVGGVGGARFLLGVKAALGLPAVGSVSEPRIDAVGAGAS
                     DHEVTAVVNVGDDIWMHGLRICPDMDTCLYTLGGGIDPERGWGRAGETWAVRDELAAY
                     EADPTWFGLGDRDLATHLVRTRMLRAGYPLSDVTAALCHRWRPGVTLLPATDDRVETH
                     VVIDDPETGPKARKAIHFQEWWVRHKAEPLAHSFASVGAEQATVLPAVRDAITGADAV
                     LIAPSNPVVSIGSLLEIPGMRGALRETTARVVGLSPIVGGRPLRGMADACLTAIGVDT
                     TAEAVGRHFGARADGGLLDGWLVHTGDSADVPGVEVLSLPLLMTDVDATAEMARRALE
                     LAGAAVA"
     gene            6434..7810
                     /locus_tag="VFE39_17495"
     CDS             6434..7810
                     /locus_tag="VFE39_17495"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012796123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HET6260434.1"
                     /translation="MRQLPDQAVTDQAADHAAPDAITVLPVRGLPEFRPGDDLGGELA
                     AAAPWITHGDVVVVTSKVFSKVEGRLVPAPTDPEARDALRRELVDAETERVLARRGRT
                     AIVAGKLGIVQAAAGIDGSNVRRDELALLPADPDASASRLRAELLRLLGVEVAVVVTD
                     TMGRSWRVGQTDVAIGSAGLEVLHRYAGSTDAEGNELLVTEVAIADELAAAADLVKGK
                     LGGLPVAVVRGMRPVDDGSTARALVRPLDEDMFWLGTDEAIARGRREAVLLRRSTRDF
                     SDEPVDEAALRRAVGVALTAPAPHHSTPIRFAWVRSAGRRDAVLHGMAARWREHLEAD
                     GRPPEEVERRMRRGDLLRRAPELVLAFRTGDGMHTYPDEARRMGERTMFTVAGGAAVQ
                     SLLVALAAEGLASCWVGSTIFAPDVVRRALDLPDDWQPLGAVAVGVPAEPLRPRPPRD
                     AAGGLVEW"
     gene            7819..8361
                     /locus_tag="VFE39_17500"
     CDS             7819..8361
                     /locus_tag="VFE39_17500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET6260435.1"
                     /translation="MAAAELRAWAPAEEGQRALRHALLAFLAARPEDACSRTCVPGHL
                     TASALVLDADGEHTLLTLHPRVGRWLQLGGHCEDSDVSLRDAALREAAEESGIDGLTI
                     DPEPLHVDVHPITCSLGVPTRHLDVQYLVRAPAGAVPRISVESDDLRWWPLDALPDDS
                     DAVATVVAAARGRRAATPAC"
BASE COUNT         1063 a         3000 c         3115 g         1200 t
ORIGIN      
        1 ggccagctcg acgtggtggg agttcactgt cccccgtcga cgatgcggag cagcgtgcgg
       61 agcgcgaggc gtcccataac ccggcatcgc caccgtggcg atctaccaga tgatcggggt
      121 gcgaaacgag ctgctgttcg ccagtgcact gatgtccgag ccggccgacc agcccatcca
      181 gccggcagcc accgccctga tcggccgaga ctccaccaac tgggccgccc tcacctccgc
      241 gatgacgttg agcgcgctgc cgggtgatcg cgtacgtggt cttccagcgc tggttcttcg
      301 ccgggctcac cgcaggcgcg gtgaagtcct gaccggtgac atcatgatcg gacaggagca
      361 gacttcgtgg acgtgctgac cccgcaaccc ctcggtccca ccgggctgaa agtgaccccg
      421 ctgtgcgtgg gcagcggcga gctcggcgac atgacctccg tccacggcta cgcggtgccg
      481 gaggagcagg ccctcgccac cgtccgggcg ttcgccggcc cgttcacgtt cgtcgacacc
      541 tccaactatg gcgcgggcac cagcgagcgt cggatcggcg cggtggcccg gtggaggtga
      601 tgcagcagct ggtcgccgag ggactggtcg acaacatcgg tgtcgcgggc ggcccggtgg
      661 acctgctgcg caaattcgtg gccaccggcg ccttcgaggt ggtcctcacg tacaaccgct
      721 ggacactgct gcaccgctcc gccgaaccga tccgcctggc cacccacccc gtccctgacg
      781 agctctggac cgtgctcgcc gacctcacac ctgccgagga gaactggctg cgatgacagc
      841 acctgccccc acaccccgca cccccaccac gttcggtgtg gtcggcagcg gctggcgctc
      901 gctgttcttc ctgcggctgg cccaggccgc ccccgaccac ctgcgggccg caggcgttgt
      961 cacccggacc gccgagcgcg gccaggaggt caccgatgcg tggggggtgc cgaccttccg
     1021 cacggtcgag gagctgctgg ccgcggatcg tcccgacttc gtcatcgcgg cggtgccgtg
     1081 ggggcagatg cccgtcacca cgcgggaggt ggtggccctc ggcgtgcccg tgctggccga
     1141 gaccccaccc gcccccgacc tcgacggcct gcgctccctg tggtccgacg tgggcgggag
     1201 cgggctcgtg caggtggccg agcagtacct gctgatgccg ggccacgccg cccggctcgc
     1261 ggtgttgcgc gacggtctca tcggcgagcc gacctcggtg cagctctcct ccacgcacat
     1321 gtatcacgcg gtctcgctca tccgcggcct gctcggcgtg gacatggacg aggccgtggt
     1381 caccgggcgc gagttcaccg cgccgctggt cgacccgctc tcatccgacg gatggaagca
     1441 ggacgcggtg cccgaggcgc gcaccaccac catcgccacc atcgacttcg gcggccgctc
     1501 cggcctctac gacttcaccg acaaccagtg gtggaacccg ttgcgcgccc gtcggatcct
     1561 cgtccgcggt tccatgggcg agctcgtcga cgaccgcctc gtgcggatgg tcgacccgac
     1621 gagccccgtg gagtcgcacc tcgtctaccg ccgcacgggc gtcgacatga acctcgaggg
     1681 caacgacctc gacttcgtga gcgtcgacgg cagggtggtg taccggaacc cgttctcggg
     1741 cacccgcctg tcggaggacg acatcgccgt ggcggcgttc ctggaggcca ccggagcgtg
     1801 ggctcgtgga gagggcccgg agccctaccc gctggcgcag ggctgccagg accacgccat
     1861 cgcactggcg atcggcgagt ccgcccgcac cgggcacgat gttcgcgtga cgaaggagcc
     1921 gtgggcctga tgccgctggt gcggaccgat ctacccgaca ccaccacgga cgagcagcgc
     1981 cgcgcgatcg cccacggcgt caaccgcggc ctcgcccgcg ggacggccgc atgagggccg
     2041 ccgtcctgcg cgagcccggc acgccattgc aggtggagga tctggaggtc gacgccccgc
     2101 atgccggcga ggtgctggtg cgggtggagg cggcgggggt gtgccacacc gagctgcact
     2161 acatgtccgg cgacatcccg tgtccgttgc cgatcgtgct ggggcacgag ggttgtgggg
     2221 tcgtcgagca catcggttcc ggggtcgaca cggtggcggt gggggaccgg gtcgcgttca
     2281 cctggcgccc acggtgcggg ttctgcgagt tctgcatgac cggccggccg gtgatgtgca
     2341 tctacggccg ggtgcaggct cgctcgggcg ggttgatgga cggcacctcg cgcctgcgtc
     2401 gcggcgagga gaaggtgcac cacttcctgg gcgtttcgtg cttcgcggag cgggcggtgg
     2461 tgtcgcagcg ggcggtggtg ccgctgcccg aggacgtgcc ggtcgcggtc gccgcggtca
     2521 ccggctgcgc ggtgatcacg gggctgggcg cggtggtgaa cgtgggcggg cagctggcgg
     2581 gccggccgct gctggtgatc ggcgcgggcg gggtcgggct gtcggcggtg atgggcgcgg
     2641 tcctggtcgg tgccggaccc gtcgtggtgg tggacgtggc ggcggacaag ctggagctgg
     2701 cacgccggct gggcgccacc cacaccgtcg acgccggcat cctcgacgcg gccggtgtgg
     2761 tggagtcggt gcaggagctg accgggggcg gcgcggaggt ggcggtcgac gccgtcggca
     2821 gcccgcagac cctgcgccag gccttcaccg cgctgcgccc cggcggcacc gcgatcgccg
     2881 ttgggctggc cgcggtggac gccaccgccg caatcccgat caacgagctg gtgcagcagc
     2941 agaaacacct ggtcggcagc ctctacggct cggccaaccc cccggtggac ctgccccgga
     3001 tcctgtcgct gtaccagtcg gggcggctcc cgctggaggc gctgctcggc gcccagcacc
     3061 ctctcgaggg cgtcgaggac gcctatcggg ccctgcgcgc cggcgccccc ggccgcgccg
     3121 tcgtggtgcc cggcccgtga ccgagtacgc cacctatccg agcctgcgcg acatggtggt
     3181 gctgatcagt cgtggggcag cggctcggcg cggagtcccc ggcgccgccg gtactgacag
     3241 ctcggggatc agctgctcgc cagttcgtgg atgacggacc gcagcggcga gctgcgccgc
     3301 acggcggccc ggatgcggct cggcgcggtg ggcgggcagc cgcaccgcgc ccactcggct
     3361 cgtggtgcgc ggctcgacgc cggccgactg acggctgctg ggagtggcat cccgctcagc
     3421 gctgctccca ccgccagttc agctcggcgg cctgtggggg tggtccgggc agcggcgggc
     3481 gggggtcgcc gtcggcggcg agtccggcca ggtgggtggc gaggacgcgg cggcgtagct
     3541 cgcgcgtgcg tccgggatcg gggacgcgga tcgccgcgca cgcctccagc gcgagcccca
     3601 cgtcctgccc gaccacgtcc ggccggagcc gcccggcggc gcgggcgcgc tcgaacaggg
     3661 cgatgccgag attgccggcc cgctcggcgt cccgctgcat ctccgcggtg gggacgaagg
     3721 tgccggccag gtgaacggtc agcgagtgga cgtcggcgtc cacgatgcgc tgcagaaaag
     3781 tgctgaacgc gctccaggcg tccgggtccg ctgcggcggc ctccgcctcg gcgaggtagc
     3841 ggcgcaaccc gtcgtggcag agcgtgcgca gcagatcctc cttcccggga tagcggcggt
     3901 agagggcgct gatgccgaca cccgcgcgtt gcgcgacggc ggcgatcggc gccttcggat
     3961 cgtcgaggaa caccgcgcgg gcggcgtcga ggatcgccgt gtcgttctgg gcggcttggc
     4021 ggcgtcgacc ggagagaccg gcttcggcgg ccgccgggat ctgcgaggac atctttggat
     4081 cctactctgg aacggatcgt tccgctctgc tatagtaaag gggaacaaaa cgttccgttc
     4141 cgaggagagc ctgtgtcgaa caccgacatc cgcccgttcc gcgtcgacat cccgcaggac
     4201 gcactggacg acctggacgc ccggctgcgc ggggcccggt ggccccacga gctgcccggc
     4261 tctgccgacg tctatggcgt gcccgtcgcc cgggtccggg agctcgtcgc gcactggctc
     4321 gacgggttcg actggcgggc ggtcgaggcc cggctgaacg cctaccccca gttcgtcacg
     4381 gagatcgacg gcgaggacat ccacttcctg cacgtgcggt caccccgggc cgacgccacg
     4441 cccgtgatcc tcacccacgg ctggcccggc accgtggtgg agttcctcga cgtcataggt
     4501 cccctgaccg agcccgcgtc ggcggatgac ccggccttcc acctggtgat cccgtccctg
     4561 cccggcttcg gcttctccgg gcccacccgc agcggcgggt ggggcacgcg tcggacggcg
     4621 cgggcgtggg tcgagctgat gagcaggttg ggctacgacc gctacggcgc gatcggtaac
     4681 gacgcaggct ccatgatctc cccggagatc gggcggatcg acggcgagca cgtcgtcggg
     4741 gtgcacgtca cgcagttctt ctcgttcccc tcgggcgacc cggccgagtt cgagaagctc
     4801 accgaggccg accacgcggc gctggcgcac ctccagtcgt tcctcgacac gaagttcgcc
     4861 ttcaacgtgc tgcacagcca gcagccgcag accctcgcct tcgccctggc cgactcgccg
     4921 gtcgggctgc tcgcctggaa cgcgcagctc atgggcgaga gcctggatcc tgacttcgtc
     4981 ctggccaacg tcgccatcta ctggttgacc ggcacagctg catcggccat ccgcttctac
     5041 tacgaggacg cccacctcgc cgaggagccc gccgctgagc ccaccgtcgc gcccaccggg
     5101 ctggccatgt tcgccgggga cttccagtcg atccgggcgt tcgctgggcg cgaccacgcg
     5161 aacatcgtca gctggaactc ctacgacccc ggcctccctg cgggcggtcg gcaggacgcc
     5221 ccgggtcatt acgccgcgca cgaggcgacc gacctgttgg tgggggacat ccggcggttc
     5281 ttcgcgcagc tgggctgaga gctgctccag gctccccggc tccgggagcg ccactttccc
     5341 gccttccggg gcagaaggtg ctttcctgac cttgccgtgc cctcggcgac ggggggcccc
     5401 ggccccggac gtaggctcgg tggggtgaag gtcacggtgc tggtcggggg cgtcggcgga
     5461 gccaggttcc tgctcggggt gaaggcagct cttgggctcc ccgccgtggg gagcgtcagc
     5521 gagccgcgca tcgatgccgt cggtgcgggg gcctcggacc acgaggtcac ggccgtcgtg
     5581 aacgtcggtg acgacatctg gatgcacggc ctacgcatct gccccgatat ggacacgtgc
     5641 ctgtacacgc tcgggggcgg catcgacccc gagcgtggct ggggccgggc cggggagacg
     5701 tgggcggtgc gcgacgagct ggccgcgtac gaggccgacc ccacgtggtt cgggctcggc
     5761 gaccgcgacc tcgccacaca cctcgtccgc acccggatgc tgcgggccgg ctacccgctc
     5821 tccgatgtca ccgcggcgct gtgccaccgc tggcggcccg gcgtcacgtt gctgcccgcc
     5881 accgacgacc gcgtggagac ccacgtcgtc atcgacgacc cggagaccgg cccgaaggcg
     5941 cgcaaggcga tccacttcca ggagtggtgg gtacgccaca aggccgaacc tctcgcgcac
     6001 agcttcgcct ccgtcggtgc ggagcaggcg accgtgctcc ccgcggtccg cgacgcgatc
     6061 acgggcgccg acgccgtgct gatcgcgccg tcgaacccgg tggtgagcat cgggtcgctg
     6121 ctcgagatcc cggggatgcg cggcgcgctg cgcgaaacga cagcgagggt cgtcggactg
     6181 tcgccgatcg tgggcggcag gccgcttcgg gggatggccg acgcgtgcct caccgcgatc
     6241 ggcgtcgaca ccacggcgga ggccgtcggt cggcacttcg gggctcgggc cgacggcggc
     6301 ctgctcgacg gctggctggt gcacaccggc gacagcgccg atgtccccgg cgtcgaggtg
     6361 ctgtcgctcc cgctgctgat gacggatgtc gacgccacgg cggagatggc gcgccgcgcc
     6421 ctggagttgg ccggtgcggc agttgcctga ccaggccgtg accgaccagg ctgccgacca
     6481 cgcggccccc gacgccatca ccgtcctgcc ggtccgcggg ctgcccgagt tccggcccgg
     6541 cgacgacctg ggtggtgagc tggccgccgc cgcgccgtgg atcacccacg gggacgtcgt
     6601 cgtggtcacg tcgaaggtgt tctccaaggt cgagggacgc ctcgtacccg cccccaccga
     6661 cccggaggcc cgcgacgcgc tgcgccgcga gcttgtggac gccgagaccg agcgggtgct
     6721 cgcgcggcgc gggcgcacgg cgatcgtcgc cgggaagctc ggcatcgtgc aggccgccgc
     6781 cggtatcgac ggctccaacg tgcggcgcga cgagctggcg ctgctgccgg ccgaccccga
     6841 cgccagtgcc tctcggttgc gggcggagct tctgcggttg ctcggggtcg aggtcgccgt
     6901 ggtcgtcacg gacacgatgg gccgctcgtg gcgggtcggg cagaccgacg tcgcgatcgg
     6961 ctcggccggg ctcgaggtgc tccaccgtta cgccggcagc accgacgccg agggcaacga
     7021 gctgctggtc accgaggtcg cgatcgccga cgagctggcg gccgctgccg acctcgtgaa
     7081 gggaaagctg ggcggcctgc cggtggccgt ggtgcgcggc atgcggccgg tggacgacgg
     7141 ctccaccgcc cgcgcgctcg tgcgaccgct cgatgaggac atgttctggc tgggcaccga
     7201 cgaggccatc gcccggggca ggcgggaggc ggtgctgctg cgccgtagca cccgtgactt
     7261 ctccgacgag ccggtggacg aggctgcact gcgccgcgcg gtcggcgtcg cgctcacggc
     7321 gcccgcgccg caccacagca cgccgatccg gttcgcttgg gtgcgctccg ccggacgacg
     7381 ggacgcagtg cttcacggca tggcggcccg atggcgggag cacctggagg ccgacgggcg
     7441 cccgccggag gaggtcgagc ggcggatgcg ccgcggggac ctgttgcgcc gcgcgcccga
     7501 gctcgtactg gccttccgca ccggcgatgg catgcacacc taccccgacg aggcccgccg
     7561 catgggcgaa cgcacgatgt tcaccgtggc ggggggcgcg gcggtgcagt cgctcctggt
     7621 ggcgctggcc gccgaggggc tggcgtcctg ctgggtgggg tccacgatct tcgctccgga
     7681 cgtcgtgcgc cgagcgctcg acctgccgga cgactggcaa ccgctcgggg cagtcgccgt
     7741 cggggtgccg gccgaaccgc tccgaccgcg gccgccgcgg gacgcggctg gggggctggt
     7801 cgagtggtag cgccggcgat ggccgcagcg gagctgcggg cctgggcacc ggctgaggag
     7861 ggacagcgcg cgctccggca cgccctgctc gccttcctcg ccgcccggcc cgaggatgcc
     7921 tgctcacgga cgtgcgtgcc ggggcacctc acggcctccg cgctggtcct cgacgcggac
     7981 ggcgagcaca cgctgctcac cctgcaccca cgggtcggac ggtggctcca gctcggcggc
     8041 cattgcgagg acagcgacgt ctcgctgcgg gacgccgcgc tgcgggaggc ggcggaggag
     8101 tccggcatcg acgggctgac gatcgacccg gagccgttgc acgtggatgt gcacccgatc
     8161 acctgttcgc tcggcgtgcc gacgcgccac ctcgacgtcc agtacctggt gcgcgcgcct
     8221 gccggggcgg tgccgcggat cagcgtcgag tccgacgatc tgcggtggtg gccgctcgat
     8281 gcgctccccg atgacagcga tgcagtggcg acggtggtgg ccgccgcgcg cggacgccgg
     8341 gccgccactc ccgcctgctg accacgccgg aagtcccc
//
LOCUS       DASRSC010000361        11619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_318003,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000361 DASRSC010000000
VERSION     DASRSC010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 11619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11619
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318003"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VFE39_17505"
     CDS             complement(<1..1008)
                     /locus_tag="VFE39_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6260436.1"
                     /translation="MDAAGSGTTTIRTAIPARLDRLPWSSFHWRIVIGLGAVWILDGL
                     EVTIVGAVASRMIEPGSGITLNAADIGTGAALYVAGACIGALFFGQLTDRLGRKKLFM
                     ITLAIYLVATVLTAFAFAPWYFFLCRFFTGVGIGGEYSAINSAIDELIPARNRGQVDL
                     AINGSFWVGSGLGALVSLLLLDASLFAPSLGWRLAFGLGFVIGLGILLVRRHLPESPR
                     WLVLRGRMEEAERIVEDIEQRVRAETGVELPPPSYEIVVRLRQNIRYRDLVAIAFRRY
                     PNRAVLGLALFIGQAFLYNAVVFDLGTLLSEVFGVSSSAVPGYMALFAASNFLGPLLL
                     GR"
     gene            complement(1206..1430)
                     /locus_tag="VFE39_17510"
     CDS             complement(1206..1430)
                     /locus_tag="VFE39_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2905 domain-containing protein"
                     /protein_id="HET6260437.1"
                     /translation="MAKLLGTWLVGGGIILVVAGLLAWAGLLSWLGHLPGDIRIVSGS
                     TRIYIPITSMLLVSLALNALLWLFLWLFNR"
     gene            2291..2719
                     /locus_tag="VFE39_17515"
     CDS             2291..2719
                     /locus_tag="VFE39_17515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HET6260438.1"
                     /translation="MSSTGYATFDTTLAKTNGVLKQIEESYGWPKERRNQSYGALRAV
                     LHALRDRLTVEEAAQLSAQLPMLIRGIYFEGWDPTKVPAKMHLDDLLARIRHEFPFEV
                     PGGPERLLGTTVEALRRHITEGEWEDIRASLPKDVVAVLP"
     gene            3136..3879
                     /locus_tag="VFE39_17520"
     CDS             3136..3879
                     /locus_tag="VFE39_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260439.1"
                     /translation="MRKATAAAGALFAAGAVFSTGLVDTVARSPNRNVDLGMAPIGPN
                     ATGGRVHWAREVGRASSGDGLEPAVVAGIFIPIEPAEADPDATARELPSAPDVGPEPS
                     PRTATSEMSDLAAVIEMPAGVELNVPIVGTISPPRFAVSDGRVRNPDLTVPASHGAVG
                     VGVSESPEVEFSEVGAVRAHLSPVAARDIGGSLCALPRDSGPLLAMAGSTVSGARAML
                     DMSSDGTTRLAHGACSRPAGVSSSCPRAS"
     gene            complement(3822..4643)
                     /locus_tag="VFE39_17525"
     CDS             complement(3822..4643)
                     /locus_tag="VFE39_17525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6260440.1"
                     /translation="MPTALVTGASRGLGAAFAQRLARDGLDLVLVARDTVGLQRVASE
                     AARRGVSVEVLAADLADATDRAAVERRVADPHRPVDLLVNNASFECGVDFRTAAAAPL
                     QTEIDVNVTAVMMLTHAALPGMIERGQGWVINVASFAGYLSASGSAHSATKAWMLAFT
                     DTVEASLSGTGVRMIALVPGRLRTGKHRDLEPVGTSLMWLDPAAVVDACLSDLARGRT
                     VCTPGLLYGAVVRTLETPRRTLRTLARLAGHRRGYMRQDARGHELETPAGREQAP"
     gene            5366..6862
                     /locus_tag="VFE39_17530"
     CDS             5366..6862
                     /locus_tag="VFE39_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260441.1"
                     /translation="MFATAVVENVVRAPRSGFELGTDGGTAGDRSTGPSSDGGRVNAQ
                     RVTFSVAELFAATAPGSSSPEIGGGVFDDLSDAATPGSAGAGGSAAPAGAGAVPGAAG
                     PVAPAPRSTGAGSAPPLDTGSGSPGPGGLLPGLLPGLLPGLLPGGGGTGGGGVPGGGE
                     GGPGVTVPLPDIGTPGVGVPGGALRDLPVVGDVRTPGIAVSPAQLRTPDLSVSAGNGA
                     VGIGVSDTALVTPDVTVTEVQAGPAGLSPAKVTVPNVGITGGGATLSGNAAPRLAVPG
                     VSVSKTEVITPDVELGNARIALPDATLPELTLPQLQVDASNPGSAVEDAASAVTDTVE
                     QAATGVVDEGVDVVGQTVESVGQTVGAVDETVGLVGETLGSLDRTSRSGSGSTNSGND
                     SGSSRSTSAGASDDSRRSGSSGISGIVGALSGSGSNNSDSDSSGKNSDDNSGGSRSDN
                     SGSSSSSSNNDSGRDSGNSGNSSRGDSGSSGGSGRSGGSGLGSILGRG"
     gene            complement(6877..8490)
                     /locus_tag="VFE39_17535"
     CDS             complement(6877..8490)
                     /locus_tag="VFE39_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET6260442.1"
                     /translation="MPTLTADAVVIGGGHHGLVAAAVLADAGWDVCVLESADVVGGAI
                     RSAQLHPGFTADLFSAFYPLSAASPVLRALELERHGLRWSHAPSVLAHPPRPDGAPAA
                     VLHRDRERTAAGLAQHDPRDGEAWLRLCSQWDAVGDAVLRTLFTAFPPVRGPVQLLRR
                     IGSAEALRLARFLALPVGRMGEELFASEAARLLLAGNAVHADAPSDATVSGVFGWLLA
                     MLGQQYGFPAPVGGSGELAVALARRATAAGADLRTGEHVERIEVSGGRAVAVHTATGL
                     SVRVRRAVVADVAAPTLYRDLLLPEVLPPRLHADLVRFVWDTPVVKVNWALDGRIPWR
                     APDVAGAGTVHVGADARGLVRWSGDIESGTVPASPFLLVGQMSTADPSRSPDGTESAW
                     AYTHLPRGVAHDEAAEELAERIDAVLEEFAPGFGARVLARSVQRPRDLQAADPNLVHG
                     AVNGGTAQLFQQLVFRPVPGLGRSETVVGGLYLGSASAHPGGGVHGVCGWLAARAALA
                     DHGSFGALRRRLTSAALELLYRDSPPTRT"
     gene            complement(8566..9150)
                     /locus_tag="VFE39_17540"
     CDS             complement(8566..9150)
                     /locus_tag="VFE39_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017981576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03086 family metal-binding protein"
                     /protein_id="HET6260443.1"
                     /translation="MDIKNRYTRAQDGFDRIVSALPLDRWDDPSACSEWSARDVLGHV
                     VWGQELVRHLATGQPFESQVGAPGAPNPGQLIGADPLGTWRAARAAADAVLTPAALAR
                     VVSLRAFGEVPLEAFVTALVTDFLAHTWDIEYPAGLDVRLDPDLVPGCFAWSRQFEMR
                     VPGGIGPELTPPAGADEQTRFLAYLGRGVPAAIG"
     gene            9308..9676
                     /locus_tag="VFE39_17545"
     CDS             9308..9676
                     /locus_tag="VFE39_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HET6260444.1"
                     /translation="MRYMLLIYGCERPDAGTPAHAAKIAAVRAFTQQCEDDGVLLASD
                     PLHTTETATTVRVRSGDTLMTDGPFAETAEQLGGYFILDCADLDEALQYAARCPMAAE
                     GSVEVRPVLDRQPAALQVSE"
     gene            9677..10924
                     /locus_tag="VFE39_17550"
     CDS             9677..10924
                     /locus_tag="VFE39_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HET6260445.1"
                     /translation="MSESGAVLDRVFRTERARVLAALIRGLGDFELAEDALSEALAAA
                     LGRWPTDGVPRDPAAWLVTVARRAAVDRIRRARVGEAKYEQVARAAGDRSAVDELPDL
                     LSEGLGRLGDDRLSLLFTCCHPALGHEAQVALTLQAVAGLTAAQIARAFLVGEATMAQ
                     RLVRAKRKIRDAGIRFAVPDDAALPDRLTGALAVIYLVFNEGYLATTGDGLLRPELAA
                     EAIRLGKMLAALMPDEAEPLGLVALMLLQDSRRDTRTGPGGGLVLLEDQDRTRWDRAQ
                     IDEGLGLVETALRRGRPGLYQLQSAIAAVHASSPTAGDTDWPQIVALYGELLRRHPSP
                     VVALNRAVAVAMVEGPQRGLELLAGIEGLEQYRLFHAARGDLLRRQDRTPEAVAAYRR
                     ALRLATNPAERRFLDGRLSALGE"
     gene            complement(10925..>11619)
                     /locus_tag="VFE39_17555"
     CDS             complement(10925..>11619)
                     /locus_tag="VFE39_17555"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family substrate-binding domain-containing
                     protein"
                     /protein_id="HET6260446.1"
                     /translation="VAEARRTLRQADRVLASAERHRRAAAGVLRVGFEASGAGELTTR
                     ARAEFARRNPGVRVEPKRFDWGQEVAALRAGRVDVAFVWLPADLTGMHSEVVHTERRV
                     VGLPIGHPLAGREGISVLDVGEEPLMWTERAPREWVDWWAVNPRPDGSSPRWGPTNDN
                     VEEMLEQVAEGTAICFCPLSMARYYARPDLAWVPLTDVEPLRVALAWVEGADTSLVRG
                     FACVVRELATKR"
BASE COUNT         1797 a         4328 c         4021 g         1473 t
ORIGIN      
        1 acggcccagc agcagcggac cgaggaagtt gctcgccgcg aacagggcca tgtagccggg
       61 caccgccgac gagctgacgc cgaacacctc gctgagaagt gtccccaggt cgaacacgac
      121 cgcgttgtag aggaaggcct gcccgatgaa cagcgcgagg cccaggaccg cacggttggg
      181 gtagcgccga aaggcgatcg cgacgaggtc ccggtagcga atgttctgcc gcaggcggac
      241 gacgatctcg taggacggcg gcggcagctc gacgcccgtc tccgcccgca cgcgctgctc
      301 gatgtcctcg acgatgcgct ccgcttcctc catccgccca cggagcacca accagcgcgg
      361 gctctccggt aagtgcctgc gcaccagcag gatgcccagg ccgatcacga agccgaggcc
      421 gaaggccagc cgccacccga ggctcggcgc gaacagcgac gcgtcgagca gcagcagcga
      481 caccagagcc ccgagccccg accccaccca gaagctcccg ttgatggcga ggtcgacctg
      541 gccccggttc cgggccggga tcagctcgtc gatcgcggag ttgatcgccg agtactcccc
      601 gccgatgccg accccggtga aaaaccggca caggaagaag taccagggag caaacgcgaa
      661 cgcggtgagc acggtcgcca ccaggtagat ggcgagagtg atcatgaaca gcttcttgcg
      721 cccgagccgg tcggtgagct gaccgaagaa caacgcaccg atgcacgcac ccgccacgta
      781 gagcgctgcg cccgtcccga tgtcggcggc gttcagcgtg atcccgctgc ccggctcgat
      841 catccgggag gccaccgcgc cgacgatcgt gacttccagg ccgtcgagga tccacaccgc
      901 gcccaggccg atcacaattc gccaatggaa cgacgaccac ggcagccggt cgagccgagc
      961 cggtattgcg gtccggatgg tcgttgttcc cgagccggcg gcgtccatga cgtgccacct
     1021 cctccaactc aggccagcga aacagccgca ttctgcgagt caccggtgcc cacgggcacc
     1081 gacccggcga cacaacccct cgacgtctcg ttcgacccgt cagcgcgacc gggagcgatg
     1141 aatgacgtcg aaaggccctc cgtgcgacgc gccggcgagg tcgatgtcgc gatcccgcag
     1201 agccgtcagc ggttgaacag ccagaggaac aaccagagaa gggcgttcag ggcgagcgag
     1261 acgagcaaca tcgatgtgat cgggatgtag atcctggtgc tcccgctgac gatccggata
     1321 tcgcccggga gatgccccag ccaggacagc aatcctgccc aggcgaggag cccggcgacg
     1381 accaggatta ttcctccgcc caccagccat gtgcccagga gcttggccat cgcacgagaa
     1441 tgcgcctgat ctccgctccc cgcatgttca atccgcccat tgtggcggaa cacgacatcg
     1501 ctgtcggatc cccgagaccg cggccattcc cctcaggatc gtcgccggct ccggtcggga
     1561 tcctcggctg aacggtagcc gtaagccgct tcgcgcacgg gggtgtggtg acgaccaaca
     1621 gcgccgcgag cggcgtgacc accccgagcg ccaccgtgag cgtcgtcgcg tggttgacca
     1681 gcgtgacacg ctcgcgcgcc gccggatcct gcggccgttc ccacagccgg tgcccggcga
     1741 tgagcgcaat gcagatcaca gcagccgaca ccaggcccac tgtcagcagg cgcggccagc
     1801 tcatggcgtc cgcgatccac gatatgggcg gtgaggcgag cccgaacacg cggtgcccgc
     1861 tgccgcgacc acgacaccgc tccgaacgcc ggcaccgaga ccacaccgac gccgaggcct
     1921 ttcgacgcga aggcggtgac cggacggcgc cggccggctc gaacggcagg tgctctcgat
     1981 cggcccctcc ctcccggtcc gcgaaccgcc gatctcgtcg gctccacgcc ggccccgttc
     2041 cgggatcagg gtgccgacga attctgcggc acgtcaccgg gcgacacctg gccccgccag
     2101 catcagtaag gacggcgcca cccactctgg cgggtccaga tgccgaccca caccacccgc
     2161 cactcccccg acgtgacccc gagggtcgaa ggggcggccc gatccatcac ctgccaggcg
     2221 caggtcatcg gacggccgat gggtcaccgt gggcggaaac cattcgcacg ccaggagaag
     2281 gtgcaccgac gtgagctcaa cgggatacgc gacattcgac acgacgctgg cgaagacgaa
     2341 cggggttctc aagcagatcg aagagtccta cgggtggccg aaggagcgcc gcaaccagtc
     2401 ctacggcgca ctgcgcgccg tcctgcatgc cctgcgcgac cggctgaccg tcgaggaagc
     2461 ggctcagctg tccgcgcagc tcccgatgct gatccgcggc atctacttcg agggctggga
     2521 cccgacgaag gtcccggcga agatgcacct cgacgactta ctggcgcgga tccgccacga
     2581 gttccccttc gaggtcccgg gcggccccga acggctcctc ggcacaacgg ttgaggcact
     2641 ccgccgccac atcaccgaag gcgagtggga ggacatccgg gcgagcctgc ccaaggatgt
     2701 cgtggccgtc ctaccctgac cggccctctg gtgacccagc gtcatccccc gtccggggcc
     2761 aaccgagtcg tcgccgtgcg taacgggcag cggtcatcgt gccactacgc ctttcagctc
     2821 tacttgttcc cccgatgagc gtattcaccg gacaactgag ccgatgagcg gtcagttctc
     2881 ggcgacaagt ggtttccccc ttccttcagg tgagggtgcc gacctgagcg acaacctcta
     2941 gccgccgagt tgatcatccg ctgctccgag ccggagcccg gcttccgccc ggaagccgag
     3001 caaaggtcga ccggagcgat atccgtggcg gccctgctgc ggagggaggg ccgtggaccg
     3061 cacgcggccg accgtccgct ccagccgcgc ggacattccc ggccgttgac gcctcccgcg
     3121 cctcttcgcc gcagcatgcg gaaggcgacc gcggcagctg gagcgctgtt cgcagccgga
     3181 gcggtcttca gtaccgggct ggtggacacc gtggcccgga gtccgaacag gaacgtcgac
     3241 ctcggcatgg cgcccatcgg ccccaacgcg actggcggcc gagtgcactg ggcgcgtgag
     3301 gtcggacgcg catcctccgg cgacggcctc gaacccgccg tggtggcagg gatcttcata
     3361 ccgatcgaac cggccgaggc cgaccccgac gcgaccgcac gcgaactccc gtccgcgccg
     3421 gacgtcggcc ccgagccgtc acctcgcacc gccacgtccg agatgtcgga tctcgccgcg
     3481 gtcatcgaga tgccggcggg cgtcgagctg aacgtcccga tcgtcggcac catcagccca
     3541 ccccggttcg ccgtgtcgga cgggcgggtc aggaaccccg acctcaccgt tccggcgagc
     3601 cacggcgccg tcggagtcgg cgtcagcgaa agccctgagg tcgagttctc cgaggtggga
     3661 gccgtccggg cacacctgtc accggtcgcc gcgcgcgaca tcgggggcag tctctgtgca
     3721 ctcccccgcg actcggggcc actgctcgcc atggccggca gtaccgtgtc cggcgccagg
     3781 gcaatgctcg acatgagcag cgacggcact acgcgcctcg ctcacggagc ttgttcgcgt
     3841 ccggccggcg tctcgagctc atgcccgcgg gcatcctgac gcatgtaccc gcggcggtgt
     3901 ccggcgagcc gcgccagcgt gcgcagcgtg cgccgtggcg tctcgagcgt cctgaccacc
     3961 gcgccgtaga gcaggccggg cgtacagacc gtgcggccgc gggcgaggtc ggacaggcag
     4021 gcatccacga ccgcagcagg atccagccac atcagtgacg tgccaaccgg ctccaggtcg
     4081 cggtgcttgc cggtccgcaa ccgaccaggg accagcgcga tcattcggac tccggtgccg
     4141 gacaaagacg cctcgacggt gtcggtgaac gccagcatcc acgccttcgt ggcggagtgc
     4201 gccgatccgg atgccgagag gtagccggcg aaactggcga cgttgatcac ccacccctga
     4261 ccgcgctcga tcatccccgg cagagctgcg tgggtcagca tcatcacggc ggtgacgttg
     4321 acgtcgatct cggtctgcag cggtgccgca gcggccgtcc ggaagtcgac cccgcactcg
     4381 aagctcgcgt tgttcaccag cagatcgacg ggccggtggg gatcggcgac cctgcgttcc
     4441 acggccgccc gatccgtggc gtcggcgagg tcggccgcga gcacctcgac gctcacaccc
     4501 cgacgggccg cctcgctcgc cacgcgctgc aggccgacag tgtcgcgcgc gacgagtacg
     4561 aggtcgagac cgtcgcgggc gaggcgctga gcgaacgcgg cgccgaggcc gcgactcgca
     4621 cccgtaacga gcgctgtcgg catgggcgca ccctacgaca gcagtctccg ggccggtcgt
     4681 ccaaacacgg ccacgtcgca cccgctcgac cgctggcctc ggcgcaacgc tctccggctg
     4741 tcgcacagat ggccctgagg cccgtcacgc accgccatgg cgggtggcca tacgacaact
     4801 tcccgcggac aggtaacggc gcgagttccg cctccgacta cgccgtacgg cctgctcacg
     4861 acacgcgcgc ggtcgacgac tgacgccacc tgagcggtga acggcattct aggcgggatg
     4921 agtggtcatc tccgctgttc aggtgagagg ggacgagcgg acgaacagca cctagcaggc
     4981 aggtgacgaa ggcgggtagg aaaacgaccc ccaccgcaag acatctccac cgacagctca
     5041 cccaacccgg agaggccccg acgtggaatc gcccgcccgc tcgaacggac accgtcatgg
     5101 atcgtcgaac agggtccccg tcgccgagtt gatcgtccgc tgcggccagt ggcgcgtcga
     5161 cccttccccg ggcgtggact acggggcgcc ggcctcggtc tcagtggggt ccctgctgcg
     5221 ccgcgaaggt cgcgcccccc attctgccga ccggccgctc cagccgcgtg ggcactcccg
     5281 gcctccggtc cagccactcg aacccccacg acggaacgtc cgcaaggcca cggccgccgc
     5341 aggcgtcttg ttcgccgctg gggcgatgtt cgcgaccgcc gtcgtggaga acgtcgtccg
     5401 ggccccgcgc agcggcttcg aactggggac ggatggcggc acagcgggcg accgttcgac
     5461 cgggccgtcg tcggacggcg ggcgggttaa cgcgcagcgg gtcacgttct ccgtggcaga
     5521 gctgttcgcg gcgaccgcgc cgggctcgtc cagcccggaa atcggtggtg gcgtgttcga
     5581 cgatctgagc gatgccgcga cacccggctc cgcaggcgct gggggatccg cggcgccggc
     5641 gggagcggga gccgtcccag gggctgcggg accggtcgct cccgcccccc gaagtaccgg
     5701 cgcgggcagc gcaccgcccc tggacaccgg ctccggttcg cccggccccg gcggcctcct
     5761 ccccggcctg ctacccggcc tgctacccgg tctcctcccc ggcggcggtg gcaccggggg
     5821 cggtggcgta ccgggcggtg gcgagggcgg gcccggcgtg accgtccccc tcccggacat
     5881 cggcacgccc ggtgtcgggg tccctggtgg tgctctgcgg gacctccccg tcgtcggtga
     5941 cgtcaggacg ccggggatcg ccgtgtcgcc ggcacagctc aggaccccgg acctctcggt
     6001 ctccgccggg aacggcgccg tcgggatcgg ggtttcggac accgctctgg tcacgcccga
     6061 cgtgaccgtc accgaggtac aggccggccc ggcaggcctc tcgccggcca aggtcacagt
     6121 gccgaacgtc gggatcacgg gcgggggcgc cacgctctcc ggcaacgccg cgcctcggct
     6181 cgcggtgccc ggcgtctcgg tgtccaagac agaggtcatc actcccgatg tggagctcgg
     6241 caacgccagg atcgccctgc cggacgcgac actgcccgag ctgacgctgc cccagctgca
     6301 ggtcgacgcg agcaatccgg gctcggccgt cgaggacgcc gcctccgcgg tgacggacac
     6361 cgtcgagcag gccgcgacgg gcgtggtgga cgagggcgtc gacgtggttg gccagacggt
     6421 cgagtccgtg ggccagacgg tcggtgcggt cgacgagacc gtcggcctgg tgggtgagac
     6481 gctcggctcg ctggacagga ccagccgctc ggggagcgga tccacgaact cggggaacga
     6541 ctccggcagc tcgaggagca catcagccgg cgccagcgac gactcacgtc gctccggcag
     6601 ctcgggcatc tccggcatcg tgggcgcctt gagcggctcg ggcagcaaca actccgacag
     6661 cgactcctcg ggcaagaact ccgacgacaa ctccggcggc tcgcgcagcg acaactccgg
     6721 cagctcgagc agcagttcca acaacgactc cggccgcgac tccggcaact ccggcaactc
     6781 cagccgcggc gactccggga gctccggcgg ctcggggcgc tcgggcggct ccggcctggg
     6841 cagcatcttg ggccggggct gagaggcccg cgagggctat gtcctggtgg gtggcgagtc
     6901 gcggtagagc agctccagcg cggcggacgt cagacggcgc cgcagcgccc cgaacgaccc
     6961 gtggtccgcg agcgcggccc tggcggccag ccagccgcag actccgtgca ccccgccacc
     7021 ggggtgggcc gacgcactgc ccagatacag cccgccgacg accgtctcgg accggccgag
     7081 ccccggaacc ggacggaaca ccagctgctg gaacagctgc gcggtgccac cgttgaccgc
     7141 gccgtgcacg aggttcggat cggcggcctg caggtcacgc ggccgctgca ccgagcgggc
     7201 gagcacgcgc gcaccgaacc cgggcgcgaa ctcctccagc accgcgtcga tgcgctccgc
     7261 gagctcctct gctgcctcgt cgtgggccac cccccgcggg agatgcgtat atgcccacgc
     7321 gctctccgtg ccgtccgggg accgggacgg gtcggccgtg gacatctgcc cgaccaacag
     7381 gaacggcgac gcggggaccg tgcccgactc gatgtccccc gaccaccgca ccagcccgcg
     7441 cgcgtccgct cccacgtgga cggtgccggc gccggcgaca tcgggcgccc gccacgggat
     7501 ccgcccgtcg agcgcccagt tgaccttcac caccggggtg tcccacacga accgcacgag
     7561 gtcggcgtgc aaccgcggtg gcagcacctc gggtagcagc aggtcgcgat acagggtggg
     7621 ggcggcgaca tcggcgacga ctgcacgccg cacccgcacc gagagtccgg tcgcggtgtg
     7681 caccgccacc gcacgcccgc cgctgacctc gatccgctcg acgtgctcgc cggtgcgcag
     7741 gtcggcaccg gcggcggtgg cccggcgggc cagagccacc gccagctccc ccgagccgcc
     7801 caccggggcc gggaacccgt actgctggcc cagcatcgcg agcagccagc cgaacacccc
     7861 gctcaccgtg gcgtccgacg gcgcgtcggc gtgcaccgcg ttgcccgcca gcagcaaccg
     7921 ggccgcctcc gacgcgaaca gctcctcgcc catccgcccc accggcaacg ccaggaagcg
     7981 cgccagccgc aacgcctccg cgctgccgat ccggcgcagc agctgtaccg ggccgcgcac
     8041 cggcgggaac gcggtgaaga gcgtccgcag caccgcgtcg cccacggcgt cccactggct
     8101 gcacagccga agccacgcct caccgtcacg cgggtcgtgc tgggccagac ccgccgccgt
     8161 tcgctcccgg tcgcgatgca ggaccgcggc gggcgcaccg tccggacgcg gcggatgggc
     8221 cagcacgctc ggagcgtgcg accaccgcag cccgtgccgt tccagctcca gcgctcgtag
     8281 cactggagac gcggcagaga gcgggtagaa cgcgctgaac aggtcggcgg tgaagccagg
     8341 gtgcagctgc gccgagcgga tcgccccgcc gaccacgtcg gcggactcca gcacgcacac
     8401 gtcccagccg gcatcggcga gcaccgccgc ggccaccagt ccgtgatggc caccgccgat
     8461 cacgaccgcg tcggcggtga gggtgggcat gacgccacta tctgctgctt cccactcggc
     8521 ggcacctcag gggcccccgt cgcgcggccg ccagcgcatt gccgctcagc cgatggctgc
     8581 cggcacgccc cggccgaggt acgcgaggaa ccgggtctgc tcgtcggctc ctgcgggcgg
     8641 ggtgagctcg gggccgatac cgccagggac gcgcatctcg aactgccgcg accaggcgaa
     8701 gcagccgggc acgaggtcgg gatccagccg gacgtcgagc ccggcggggt actcgatgtc
     8761 ccacgtgtgg gccaggaagt cggtgaccaa cgccgtcacg aacgcctcaa gagggacctc
     8821 gccgaacgcc cgcaacgaga cgacgcgggc gagcgccgcc ggcgtcagga cggcgtcggc
     8881 ggcggcgcgc gccgcgcgcc aggtgccgag cgggtcggca ccgatcagct gcccggggtt
     8941 cggcgctccg ggcgccccga cctgggactc gaagggctgc ccggtggcca ggtgtcggac
     9001 cagctcctga ccccacacga cgtgcccgag cacgtcacgg gcgctccact cgctgcacgc
     9061 ggacggatcg tcccagcggt cgagcggcag cgcggacacg atccggtcga acccgtcctg
     9121 ggcgcgggtg tagcggttct tgatgtccat cggtagctcc ctgcagtcgt ggtgcggaca
     9181 gctcagagga cggatgagga cagcagaact cgacacctgc ccggtgtcga tttcccggat
     9241 ccgcgttcgt cctgggggtg cggctaccgt ccggtggacc gcgacggctc acaggagggt
     9301 tgtgtccatg cgctacatgc tgttgatcta cggatgcgag cgaccggacg cgggcacccc
     9361 cgcccacgcc gccaagatcg cggcggtccg cgcgttcacg cagcagtgcg aggacgacgg
     9421 cgtactcctg gcgtccgatc ccctgcacac cacggagacc gcaacgaccg ttcgcgtgcg
     9481 cagcggcgac actctgatga ccgacggccc cttcgccgag acggccgagc agctgggcgg
     9541 ctacttcatc ctcgactgcg cagacctcga cgaggcgctc cagtacgccg cgcgctgccc
     9601 catggccgca gagggctcgg tcgaggtgcg accggtcctc gatcggcagc ctgccgccct
     9661 gcaggtgtcc gagtaggtgt ccgagtccgg cgccgtcctg gaccgggtgt tccgcacgga
     9721 gcgggcccgc gtgctcgccg cgctgatccg gggcctcggt gacttcgagc tcgccgagga
     9781 cgcgctgtcg gaggccctcg ccgcggcact gggccgctgg cccaccgacg gggtgccgcg
     9841 cgacccagcg gcctggctgg tgaccgtcgc gcggcgggcc gccgtggacc gcatccggcg
     9901 cgcccgggtg ggtgaggcca agtacgagca ggtcgcgcgg gcggccgggg accggagtgc
     9961 ggtggacgag ctgccggacc tgctgtcgga gggcctcggc cgcctcggcg acgatcggct
    10021 gagcctgctg ttcacctgct gccaccccgc gttgggccac gaggcgcagg tcgcactgac
    10081 cctgcaagcc gtcgccggcc tgaccgcggc ccagatcgcc agggccttcc tcgtcggcga
    10141 ggcgacgatg gcccagcggc tggtgcgagc caaacgcaag atccgcgacg ccgggatccg
    10201 cttcgccgtg ccggacgacg ccgcgctccc cgatcggctg acgggcgcgc tggccgtcat
    10261 ctacctggtc ttcaacgagg gctacctcgc caccacgggc gacgggctgc tccggccgga
    10321 gctcgccgcc gaggcgatcc ggctgggcaa gatgttggcc gcgctcatgc ccgacgaagc
    10381 agagccactc gggctggtcg cattgatgct gctgcaggac tcgcggcgcg acacccgcac
    10441 cgggccggga ggcgggctcg tactgctcga ggaccaggac cgcacccggt gggaccgggc
    10501 gcagatagac gagggtctcg gcctggtcga gaccgccctg cgccgcggcc ggcccgggct
    10561 ctaccagctc cagtcggcga tcgccgccgt acacgcatcg tccccgaccg cgggcgacac
    10621 cgactggccc cagatcgtcg ccctttacgg cgagctgctc aggcggcacc cgtcgccggt
    10681 ggtcgcgctc aatcgggcgg tggccgttgc catggtcgag ggcccgcagc gcgggctgga
    10741 gctgctggcc gggatcgagg gtctggagca gtaccggctc ttccacgccg cgcgcggtga
    10801 cctgctgcgc aggcaggacc ggacgcccga ggccgtggcg gcctaccggc gggcgctccg
    10861 gctggcgacc aacccggccg agcgccggtt cctcgacggc cggctcagcg cgctcgggga
    10921 atgatcaccg cttggtggcg agctcgcgga cgacgcaggc gaacccccgt accagcgacg
    10981 tgtcggcgcc ctcgacccag gcgagggcca cccgtagcgg ctcgacgtcg gtgagcggca
    11041 cccaggccag gtcggggcgg gcgtagtacc gggccatgct cagcgggcag aagcagatcg
    11101 cggttccctc cgccacctgc tcgagcatct cctcgacgtt gtcgttggtc ggcccccacc
    11161 gcgggctcga tccgtcggga cgcgggttga ccgcccacca gtccacccac tcgcgcggcg
    11221 cccgttcggt ccacatcagc ggctcctcgc ccacgtcgag cacggagatg ccctcgcgcc
    11281 cggcgagcgg gtggccgatg ggcagaccga cgacccgccg ctcggtgtgc accacctcgg
    11341 aatgcatgcc ggtgaggtcc gccggcagcc agacgaaggc cacgtcgacc cggccggcac
    11401 gcagcgcggc gacctcctgc ccccagtcga accgtttggg ctccacccgc acgcccgggt
    11461 tccgccgggc gaactcagca cgcgccctgg tggtgagctc gcccgcgccg ctggcctcga
    11521 atccgacccg caacaccccg gccgccgcgc gccggtgccg ctcggctgag gccagcacgc
    11581 gatcggcctg ccgcagggtg cgccgcgcct cggccacga
//
LOCUS       DASRSC010000362        12756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4738090,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000362 DASRSC010000000
VERSION     DASRSC010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12756
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4738090"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3631)
                     /locus_tag="VFE39_17560"
     CDS             complement(<1..3631)
                     /locus_tag="VFE39_17560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HET6260447.1"
                     /translation="MGQTRDETTAASDASDAGLADLCRLYSRHLMAEGPGSDELPEAD
                     AVLLAVVRAHRRLAVRRAQGESLIEVSGPGPQDTGPVVGVVTDEMPFVVESLLAGLDR
                     LDAKVRRVIHPTVVVRRDVLGDLTEVLGSPPADQRASGALAEAWIRVELDPFPADEAD
                     ELAAELRLVLRDVRDVALDGAQILDAVQGVAADLADLSPGSPLAEVEDARRLLHWLVD
                     GRFSVLGYLRYDSDCVDGEHFELRAAPAAGLGVLRRDDVAARVFHEASDADGSVAHRV
                     LVLTRASAPSRVFRPVYPSLLAIRLVDGAGRVVAEHRFLGALTAAALHENVLDIPGIE
                     RRVRAAINSAGSPLESYAGQRMLEVISEYPREELFWAGEQSLRDTAVGVLALTQPRRL
                     RLFLAREPYRRFFSCLVYLPRDRYSTRARLAMQNVLVNELHGWRIEHTASVGGSSLAL
                     VHFTVHSDPAAAPPDQLRLQGQLAAAIMTWDEWVLDVAGADDDEIADYVAGVPEGYKD
                     AVDPVGALADLRVVRDLGEEPELQLSIEPGTHGAELRFRLFLLGPGVTLSTVLPVLQS
                     LGVEVLDERPHEINRPDGSRCWIYDFGLRLDAATRDAMAGRTAAETQRLFCAAFLAAW
                     RGDAEVDGFNALVLRAGLPWQDAALLRAYARYTAQLGSPFGLQYTVDTLIAHPSVTAA
                     LVRLFHARFDPVPDAADRHRAHVEALAGATALIDEVPGLDADRILRAYLNVIEATLRT
                     NYYRRRPFFSFKFDSGAVLDMPAPRPRFEIFVYSPRVEGVHLRFGAVARGGLRWSDRL
                     QDYRTEILGLAKAQAVKNAVIVPVGAKGGFVVRRSDADPAEVAACYRTFICGLLDVTD
                     DRAIVGEREKTVPPPGVVRHDGDDSYLVVAADKGTARFSDLANEVAASYGFWLGDAFA
                     SGGSVGYDHKAMGITARGAWESVKRHFRELGVDTQRESFSVVGVGDMSGDVFGNGMLL
                     SPHIRLLAAFDHRHVFVDPDPDSDRSHAERKRLFGLPRSSWDDYDRDAISPGGGVWLR
                     TAKSVPVGPQMRASLGIAEGVDSLSPPELIRAVLRAPADLLWNGGIGTYVKARGESHA
                     DAGDKANDAVRVDGRELRVKVVGEGGNLGLTQLGRIEFARAGGKINTDAIDNSAGVDC
                     SDHEVNIKILLDRLVVAGELEREARNKLLAAMTDEVAELVLADNRAQNALLGFSLTIA
                     AETAAV"
     gene            3898..4692
                     /locus_tag="VFE39_17565"
     CDS             3898..4692
                     /locus_tag="VFE39_17565"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HET6260448.1"
                     /translation="MGGIAEHERQQEVLRLLDTAGRVSVADLVRRFGVTAVTIRKDLE
                     VLERRHLLARVRGGAVSAEGADEGAFEMRLRHGTHAKTAIARAAAALVPDGAAIALDC
                     STTCYYLAHELRGRRSLIVVTNGLRAAESLSDSGVTVVLPGGTLRRSSWSLVGDFGDL
                     VASRGRLAMGFFGVRSLSREHGLMELSVEETTVKRRLAAACTEIYGLFDSSKIGRFAL
                     HSFAPAHRITALYTDDGAAPDALQGFADLEIQVHRVPVETAAGGAA"
     gene            4689..6218
                     /locus_tag="VFE39_17570"
     CDS             4689..6218
                     /locus_tag="VFE39_17570"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020667369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HET6260449.1"
                     /translation="MRHRTVIAVDLGAESGRVVACSFDGERVDLEVVHRFPNGASDVD
                     GHLRWDVERLWSEITDGLGRAGRGTDRIDSVGVATWGIDYGLLDADGLLIDQPVSHRD
                     ARTDGRLAEAAARVGRDRLYDATGTQLMECNTVFQLLADVAAGRLDGAATLLMVPDLF
                     HQRLSGTTVAEFTAVTTTGAYDVAARRWATELLDDLGIPTHMLPDVADAGTDLGPVLP
                     AAVGHPAYSRARVVLPGSHDTASAVAAVPFTHPDAAYVSSGTWSLVGAETPEPVVTPA
                     ARAANLTNEGGVYGTNRLLRNVAGLWVLQECRRQWAREGLPLTYAEIVARAEQATPRK
                     SHVDVDHPGFLAPGDMPARIRAECRRTGQPEPQDVGEVARCVLESLALRYRAAIDAVG
                     SVTGRPAALVHVVGGGSRNGLLNQLTADVTGLPVQAGPVETTALGNALVQLVALGELS
                     GLTDARELVRAGEPTLRYEPQLDQGWDEIYESSLGHAADDPEMPARPLESDKEGSDHA
                     R"
     gene            6208..7236
                     /locus_tag="VFE39_17575"
     CDS             6208..7236
                     /locus_tag="VFE39_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016332010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HET6260450.1"
                     /translation="MRVRRSITAAVAVALAGVVALSGCSQKNPDAAAGGGGGAKRVSV
                     AFVPKLQGIPYFEAMNTGGQRAAQELGIDWIYRGSTTADPGAQTEIVNSLIQQRVDVL
                     VVAPNDPDSMAPVLQEARDAGIHVMTSDTDAPGSVREAFVNQATADGVGTALTDALLG
                     AMGGSGKYAIVSCGQTAANLNSWIEVQRKVTAEKYPNAEIVDVVYAGEDEAQSVAMAK
                     DLMNAHPDLTGLVGECTASAPGVAKAVTEAGKVGQVFTVGLGTPQSMKQYLDNGSSSA
                     AILWDVEQLGYLTAWAGKQLAAGTPLQPTNNVSSALPAVQWTDSDKQLLLGNPLRITK
                     DNVDQYKY"
     gene            7240..8802
                     /locus_tag="VFE39_17580"
     CDS             7240..8802
                     /locus_tag="VFE39_17580"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HET6260451.1"
                     /translation="MAETITEQDHRALLRLHGVQKRYGGVRALRGVDFEVHAGEVHAL
                     VGENGAGKSTLIKIVSGAEVADEGSVEVDGVPLGGGSTQAAMEAGIATVYQEPHLFGE
                     LTVAENVFLGRELLAGGRVDWATQRGRVGELLEQIGLDRAIGDVRVADLPVAEQQLVS
                     IAKAFAHSAKVLILDEPSAILTDREIEVLFGVVRSLRAAGMGIIYISHRLDELAQISD
                     RVTVMRDGEVVATRPTRSLSVRQVAELMVGHELRADTADRAVPTGDPVLDVAALGRSG
                     AFEALDLQVRPAEVVALYGLVGSGTDAIARALYGIEPADSGAVRVDGRAVRLHTARDA
                     AAAGIAMLPGNRKQQGVFADKSIAFNISSAHLRYLAKFGFWFDRARERSLAQGFLTRI
                     AIKAPGPDTAVGALSGGNQQKVVLARQLVRAPRVLVLEEPTQGVDVGAKEEIHRLVLE
                     LADGGSAVLVISTDLPEVLQLADRVLVVRRGAMVREFGRGASQAEVLAAAAGDNGDDP
                     TSVAADAEGART"
     gene            8799..9809
                     /locus_tag="VFE39_17585"
     CDS             8799..9809
                     /locus_tag="VFE39_17585"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6260452.1"
                     /translation="MTATTGQVDQVPPVTRRRPRVRAIEGQEVALIGVTGLLWLVLSL
                     ATDTFLTSGNLQNILFTVAPIALIGIGMTAVIVTAGIDVSVGSQAAVVMVVVALLVRD
                     AGFAFLPALAVAVVSGLVLGALNGLIVAYGGIHPIIVTFGTLNIYRFVALQIFGSEQV
                     AGVPGAFAPIGGGAEGRVFGVPVAWWLTLVLAAGMWCYMRFWATGRHLYAVGGDAFAA
                     RLAGVRVRRRQISAYVLTGACVGLAACVLIGSGGLVQQNVGAGLELRVIAAVVIGGTS
                     IIGGRGTVLGTLLGALLVGTVTSAVTLLSWPTELTQLFIGGFILVAVGVDLLRERRRS
                     AR"
     gene            9806..10882
                     /locus_tag="VFE39_17590"
     CDS             9806..10882
                     /locus_tag="VFE39_17590"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET6260453.1"
                     /translation="MTDTRVPSAPGAAPPGGEGLAQRVLRLALTERVVLLAVLLVVVV
                     VWFRVLGDGGYLVAPYDVRYLGNALESMVPFALLAIAELFVIVSGRGGIDLSVGAIVS
                     LAGMVFGLMVGEWGWPLLPSIVACVAVGGLLGAVNGALVAYLRFPALIATLATYYAYS
                     SLALVSNANAPISTPPIVALHSLTRNVHLPLLPPVPLHVFTILVPCAVLAWVVLSRST
                     YGRSLYAVGTNDIAAGYATQPVTRTRFTAYVVSGLLSGVVALVTVAQFASARPDAGVV
                     GNGMALPAITIAVLGGVAIQGGIGRVGGVVVAALLVTWLNAGMLLAFEGSEGSQYQLL
                     ALGLVLVLSALLNTLALRRYGRLA"
     gene            10879..11655
                     /locus_tag="VFE39_17595"
     CDS             10879..11655
                     /locus_tag="VFE39_17595"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HET6260454.1"
                     /translation="MTFTARPTEVPDELVALTRRLGDPAADLVVLAEGNTSVRLDDDR
                     FAVKASGSRMDAADTADFVVGELAELIAVLENGAVDQDGLTRLLSAGPTRASIETLVH
                     VAALAAGAEWVAHTHPTAVVGLLAVTDAEALWSAPLFPDEAVVLGEPAWVPYATPGIM
                     LGRSVHAAIHERLDRDGVPPRLVLLGNHGIVALGATAAEVETVTTMAVKAARVRAVAL
                     SAGTPAPLEVRHARELAGRPDEAARRALLGRSPTRSQEVP"
     gene            11652..12518
                     /locus_tag="VFE39_17600"
     CDS             11652..12518
                     /locus_tag="VFE39_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020498170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HET6260455.1"
                     /translation="MNPLGIHALVWAGGWSRAECERAVRSTREAGYDLIEIPALDPAA
                     IDAAHTKAVLAEHGIRGVCSLGLDLDADISSPYPDVVMRGEAKLADALAVVTGIGGDY
                     LGGAVYSALAKYDRPLDPRGRENMVAGLRRLARSAADHGVTVGLEPVNRYESNAINTV
                     ADALAVIEEVGEPNVVVHLDVYHANIEERDFVSPVVAAGDRLGYVHVGESHRGYLGTG
                     TIDFPAVFGALAAAGYTGPVTFESFSRAVVSPDLSDRLAVWRDLWSDGMDLAVHARGF
                     VADGLATAKARA"
BASE COUNT         1828 a         4637 c         4460 g         1831 t
ORIGIN      
        1 gcaccgccgc cgtttctgcg gcgatggtga ggctgaaccc gagcagcgcg ttctgcgccc
       61 ggttgtcggc cagcacgagc tcggcgacct cgtcggtcat cgcggccagc agcttgttcc
      121 gcgcctcccg ctccaactcg ccggccacga ccaaccgatc cagcaagatc ttgatgttga
      181 cctcgtggtc ggagcagtcg acccccgccg agttgtctat ggcatcggta ttgatcttgc
      241 cgccggccct cgcgaactcg atccgcccaa gctgggtcaa cccgagattg ccaccctccc
      301 ccacgacctt gacccgcagc tcccgcccgt ccacgcgaac cgcgtcattc gccttgtcgc
      361 cggcatcggc gtgcgactcg cccctcgcct tcacataggt gccgataccg ccgttccaca
      421 gcagatcagc cggtgcccgc aacaccgccc ggatcaactc cgggggactg aggctgtcga
      481 ccccctcggc gatccccaac gaagcccgca tctgcggacc gaccggcacc gatttcgctg
      541 tgcgcagcca caccccacca cctgggctga tcgcatcccg gtcatagtcg tcccacgacg
      601 aacgcggtaa tccgaacaac cgtttacgct ccgcatgact ccgatccgaa tccgggtccg
      661 gatcgacgaa cacatgccgg tgatcgaacg cggccagtag ccggatgtgc ggcgacagca
      721 gcatcccgtt gccgaacaca tcaccggaca tgtccccgac acccaccacg ctgaacgact
      781 cccgctgggt gtccaccccc agctcccgga aatgccgttt cacgctctcc cacgcaccgc
      841 gggcagtgat acccatcgcc ttgtggtcgt acccgacgga accaccggag gcgaacgcgt
      901 caccgagcca gaaaccgtag gaagccgcca cctcgttcgc caggtccgag aaacgcgccg
      961 tccccttgtc cgcggccacc accagatagc tgtcgtcacc gtcgtgacgc accactcccg
     1021 gcggcggcac cgttttctcc cgctcgccga cgattgcacg atcgtcggtc acatccagca
     1081 acccacagat aaacgtccga tagcacgcgg ccacctccgc ggggtccgcg tccgaccggc
     1141 gcaccacgaa gccgcccttc gcccccaccg ggacgatcac cgcgttcttc accgcctgcg
     1201 cttttgccag gcccagaatc tccgtgcggt agtcctgcag ccggtccgac caccgcaacc
     1261 caccccgagc caccgcgccg aaccgcagat gcacaccttc cacccgcggc gagtacacga
     1321 agatctcgaa cctcggccgc ggtgccggca tgtccagaac cgcgcccgag tcgaacttga
     1381 acgagaagaa cggtcgcctg cggtagtagt tggtgcgaag cgtcgcctcg atgacgttca
     1441 ggtaggcccg caggatccgg tccgcgtcca accccgggac ctcgtcgatc agcgcggtgg
     1501 ccccggcgag cgcctcgacg tgggctcggt gccggtcggc ggcgtcgggc accggatcga
     1561 accgggcatg gaacagacgt accagcgcag ctgtcaccga tgggtgggcg atgagggtgt
     1621 ccacggtgta ctgcagcccg aacgggctac ccagctgcgc ggtgtaccgg gcgtacgcac
     1681 gcaacagcgc cgcgtcctgc cacggcagtc ctgcgcgcag caccagggca ttgaacccgt
     1741 cgacctcggc gtcaccgcgc catgccgcga ggaaggccgc gcagaagagg cgctgtgtct
     1801 ccgcagcggt gcggccggcc atggcatcgc gggtcgcagc gtcgagccgc agcccgaagt
     1861 cgtagatcca gcagcggctc ccgtcgggcc ggttgatctc atggggccgc tcgtcgagca
     1921 cctccacccc gaggctctgc agcacgggaa gcaccgtgga cagcgtgaca cccgggccca
     1981 gcaggaacag gcggaaccgc agctcggccc cgtgggtgcc gggctcgatc gacagctgca
     2041 gctccggttc ctcacccagg tctcgaacga cccgcaggtc ggcgagcgcc ccgaccgggt
     2101 cgacggcgtc cttgtagccc tccggcaccc ccgccacata gtccgcgatc tcgtcgtcgt
     2161 ccgccccggc gacgtcgagc acccactcgt cccacgtcat gatcgcggcg gcaagctgac
     2221 cctgtagacg gagctggtcg ggcggtgcgg ccgccggatc gctgtgcacg gtgaaatgaa
     2281 ccagcgcgag gctcgaccct ccgacgctgg cggtgtgctc gatccgccac ccgtgcagct
     2341 cgttcaccag cacgttctgc atggccagcc gggcgcgcgt ggagtatcgg tcgcgcggca
     2401 ggtagaccag gcaggagaag aagcgccgat agggctcacg cgccaggaac agccgcagcc
     2461 ggcgcggctg cgtgagtgcc agcactccga ccgccgtgtc acgcaggctc tgctcaccgg
     2521 cccagaacaa ctcctcgcgc gggtactcgg agatcacctc gagcatgcgc tgccccgcgt
     2581 aggactccag cggggatccg gccgaattga tcgcggcccg gacccgccgt tcgatccccg
     2641 ggatgtccag cacgttctcg tgcagcgcgg ccgccgtcag cgcaccgagg aagcggtgct
     2701 ccgccaccac ccgccccgcg ccgtcgacga gcctgatcgc gagcaacgac gggtacaccg
     2761 ggcggaacac gcggctgggc gcgctcgctc tggtgagcac cagcacccgg tgggcgacgg
     2821 agccgtccgc gtccgacgct tcgtggaaca cccgggcggc aacatcgtcg cggcgcagaa
     2881 caccgaggcc cgcggccggt gccgcccgta gctcgaagtg ttcgccgtcg acgcagtcgc
     2941 tgtcgtagcg gagatagccg aggaccgaga accgcccgtc gaccagccag tgcagcagac
     3001 gacgtgcatc ctccacctct gccaggggcg atcccggcga gaggtcggcc agatcagcag
     3061 ccactccctg caccgcatcg aggatctggg ccccgtccag cgccacatca cgcacgtcac
     3121 gcagcaccaa ccgcagctcc gccgccagct cgtccgcctc gtcggcgggg aacgggtcca
     3181 gctcgacgcg tatccacgcc tcggccagcg cgccggatgc ccgctgatcc gcaggcggcg
     3241 agccgagcac ctcggtgaga tcgccgagca catcgcgccg cacgaccacg gtcggatgga
     3301 tcacccggcg gaccttggcg tccagcctgt cgaggcccgc gagcagggac tccaccacga
     3361 acggcatctc gtcggtgacg acccccacga ccggcccggt gtcctgcgga ccaggtccgc
     3421 tgacctcgat cagcgactcg ccctgcgcgc gacgcacggc gagtcgccgg tgcgcacgca
     3481 caaccgcgag gagcacggcg tcggcttcgg gcagctcgtc cgaacccgga ccctcggcca
     3541 tgaggtgacg ggagtagagc cggcacagat cggccaggcc ggcgtcactc gcgtcgctcg
     3601 ccgcggtggt ctcgtcgcgg gtctgcccca tcgccttccc ccgaactcag tccgacggcg
     3661 gagcctggca ccttagtact gcaacggcgg ttccggctgg tctgtgccgg cggggagatc
     3721 aaccggggcg cacaccgaaa gccggtgggc gccgtgagtg agcaaaacga aacccggacg
     3781 ctggccgaac ggcagtgatg cgccggctgt ttcttctccc ttcattgacg ggttacgggg
     3841 atgtttccta aggtccaccg cgggtgaccg gggtcaccgg tactgggggt gggtccggtg
     3901 gggggcatcg ccgagcacga gcggcagcag gaggtcctgc gcctcctcga caccgccggc
     3961 cgcgtgagcg tcgccgatct cgtccgccgg ttcggggtca ccgcggtgac gatccgcaag
     4021 gacctcgaag tgctggagcg ccgtcacctg ctcgcccgtg tccgcggcgg cgccgtctcg
     4081 gcggagggcg ccgacgaggg ggcgttcgag atgcggctga ggcacggcac gcacgcgaag
     4141 acggcgatcg cccgcgccgc ggccgcgctg gtgcccgacg gggcagcgat cgccctggac
     4201 tgcagcacca cctgctacta cctcgcccac gagctccggg ggcggcgcag cctcatcgtc
     4261 gtcacgaacg gcctgcgggc ggccgaatcg ctcagcgact ccggcgtcac cgtcgtgctc
     4321 cccggcggca cgctgcgccg ctcgtcctgg tcactggtcg gcgacttcgg cgacctcgtg
     4381 gcgagccgcg gccggctcgc catgggcttc ttcggcgtcc gctcgctgtc ccgggagcac
     4441 ggcctgatgg agctctcggt cgaggagacg actgtgaagc gcaggctcgc cgcggcctgc
     4501 acggagatct acgggctctt cgactcctcc aagatcggtc gcttcgcgct gcacagcttc
     4561 gcgccggcac accgcatcac ggcgctctac accgacgacg gcgccgctcc cgacgcgctg
     4621 cagggcttcg cggacctgga gatccaggtg caccgggtgc cggtcgagac cgccgcgggt
     4681 ggggcggcat gaggcaccgc acagtgatcg cggtcgacct cggcgccgag agcggcaggg
     4741 tcgtcgcctg cagcttcgac ggcgagcgcg tcgacctgga ggtggtgcac cgctttccca
     4801 acggcgcgag cgacgtcgac ggccacctcc gctgggacgt cgagcggctc tggagcgaga
     4861 tcaccgacgg gctggggcgc gccgggcgtg gcacggaccg gatcgacagc gtcggcgtcg
     4921 ccacctgggg catcgactac ggcctgctgg atgccgacgg tttgctgatc gatcagccgg
     4981 tgtcgcaccg ggacgcgcgc accgacggac ggctcgcgga ggccgccgcg cgtgtcggcc
     5041 gcgaccgtct ctatgacgcc accggcaccc agctcatgga gtgcaacacg gtcttccagc
     5101 tgctcgccga cgtggccgcg ggccggctcg acggcgcggc gaccctcctg atggtcccgg
     5161 acctgttcca ccagcggctg agcggaacca ctgtcgccga gttcaccgcc gtcaccacca
     5221 ccggcgccta cgacgtggct gcgcggcggt gggccaccga gctgctcgac gacctgggca
     5281 tccccacaca catgctcccc gacgtcgccg acgcgggcac cgacctcggg cccgtgctcc
     5341 cggccgccgt cggccacccc gcctactcca gggcccgggt ggtgctgccc ggcagccatg
     5401 acaccgcctc cgcagtggcc gccgtgccgt tcacgcaccc cgacgccgcg tacgtctcct
     5461 ccggcacctg gtcgctggtc ggcgccgaga cgccggagcc cgtcgtgacc ccggcggccc
     5521 gcgccgcgaa cctgaccaac gagggcggcg tgtacggcac gaaccgcctg ctgcgcaacg
     5581 tcgccggtct gtgggtgctc caggagtgca ggcggcagtg ggcgcgtgag ggtctcccgc
     5641 tgacgtacgc ggagatcgtc gcccgcgcgg agcaggcgac gccgcggaag agccacgtcg
     5701 acgtcgacca tcccggcttc ctggctcccg gcgacatgcc cgcgcgcatc cgcgccgagt
     5761 gtcgccgcac ggggcagccg gagccgcagg acgtcggtga ggtcgcccgg tgcgtgctgg
     5821 agagccttgc gctgcgatac cgcgccgcga tcgacgccgt cgggagcgtg accggccggc
     5881 ctgccgcact cgtgcatgtg gtcggcggcg gctcgcgcaa cgggctgctc aaccagctca
     5941 ccgccgatgt gacgggcctg cccgtgcagg caggccccgt ggagacgacg gccctgggta
     6001 acgcgctcgt acagctcgtc gcgctcggcg agctgtccgg cctgaccgac gcgcgcgagc
     6061 tcgtccgggc gggcgagccg accctgcgct acgaaccgca gctcgatcag ggctgggacg
     6121 agatctatga gtcgagcctc ggccatgcgg ccgacgaccc cgagatgccg gcacgtccgc
     6181 tggagtcaga caaggaaggg agcgatcatg cgcgttaggc gctcgatcac ggcggcggtg
     6241 gccgtggcac tggcgggggt ggtggccctc tccggctgca gccagaagaa tccggacgcc
     6301 gcggccgggg gcggcggcgg cgcgaagcgg gtcagcgtcg cgttcgtacc caagctgcag
     6361 ggcatcccct acttcgaggc gatgaacacc ggagggcagc gggccgcgca ggagctcggc
     6421 atcgactgga tctaccgcgg ctcgaccacc gccgaccccg gcgcgcagac cgagatcgtg
     6481 aactcgctga tccagcagcg ggtcgacgtc ctggtggtcg ccccgaacga cccggactcg
     6541 atggccccgg tgctgcagga ggcccgcgac gccggcatcc acgtgatgac cagcgacacc
     6601 gacgcccctg gctccgtccg cgaggcgttc gtcaaccagg ccaccgcgga cggcgtcggt
     6661 accgcgctca ccgacgcact cctgggcgcg atgggcggct ccggcaagta cgcgatcgtg
     6721 tcgtgcggcc agaccgcggc gaacctgaac tcctggatcg aggtccagcg gaaggtgacg
     6781 gcggagaagt acccgaacgc cgagatcgtc gacgtcgtct acgcgggcga ggacgaggcg
     6841 cagtccgtcg ccatggcgaa ggacctcatg aacgcccacc ccgacctcac cggcctggtc
     6901 ggcgagtgca cggcgtcggc accgggcgtc gcgaaggccg tcaccgaggc cggcaaggtc
     6961 ggtcaggtct tcacggtcgg gctcggcacc ccgcagtcga tgaagcagta cctggacaac
     7021 ggctcgtcct cggcggcgat cctgtgggac gtcgaacagc tgggctacct caccgcgtgg
     7081 gcgggcaagc agctcgccgc tggcacgccg ctccagccga ccaacaacgt cagctccgcc
     7141 ctgccggccg tgcagtggac cgactcggac aagcagttgc tgctcggcaa cccgctacgg
     7201 atcacgaagg acaacgtcga ccagtacaag tactgaccga tggcggagac catcaccgag
     7261 caggaccacc gagccctgtt gcggctgcac ggcgtgcaga agcgctacgg cggtgtgcgc
     7321 gccctgcgcg gcgtcgactt cgaggttcac gcgggcgagg tgcacgcact ggtcggtgag
     7381 aacggcgcgg gcaagtccac gctgatcaag atcgtgtccg gggccgaggt cgccgacgag
     7441 ggcagcgtcg aggtggacgg cgtgccactc gggggcggca gcacccaggc cgcgatggag
     7501 gcggggatcg ccaccgtcta ccaggagccg cacctgttcg gcgagctcac ggtggccgag
     7561 aacgtgttcc tcgggcgcga gctgctggcg ggcgggcggg tcgactgggc cacccagcgg
     7621 ggcagggtgg gcgagctgct ggagcagatc gggctcgacc gggcgatcgg ggacgtccgc
     7681 gtcgccgacc tcccggtggc cgagcagcag ctcgtctcga tcgcgaaggc gttcgcgcac
     7741 tcggccaagg tcctgatcct cgacgagccg tcggcgatcc tcaccgaccg cgagatcgag
     7801 gtgctcttcg gcgtggtccg cagcctgcgg gccgcgggca tggggatcat ctacatcagc
     7861 caccggctgg acgagctcgc gcagatcagc gaccgggtca ccgtgatgcg ggacggcgag
     7921 gtggtggcaa cccggccgac gcggtcgctg tcggtccggc aggtggccga gctgatggtc
     7981 ggccatgagc tccgcgcaga caccgccgac cgggccgtcc ccacgggcga tccggtgctg
     8041 gacgtggcgg ccctgggccg gagcggggcg ttcgaggcgc tcgacctgca ggtgcgaccg
     8101 gccgaggtgg tcgcgctgta cgggctggtc ggctcgggca ccgacgcgat cgccagggcg
     8161 ctctacggca tcgagcccgc cgactcgggc gctgtgcggg tggacggccg ggcggtccgg
     8221 ctgcacaccg cgcgcgacgc cgcggcggcc gggatcgcca tgctgcccgg caaccggaaa
     8281 cagcagggcg tgttcgccga caagtccatc gccttcaaca tctccagcgc ccacctgcgc
     8341 tacctggcga agttcgggtt ctggttcgac cgcgcccgcg aacggtcgct cgcgcagggc
     8401 ttcctcactc gcatcgcgat caaggcgccg ggccccgaca ccgccgtcgg tgccctctcc
     8461 ggtggcaacc agcagaaggt cgtgctggcc cgccagctcg tacgggcccc ccgggtgctc
     8521 gtgctggagg agcccacgca gggcgtcgac gtcggggcga aggaggagat ccaccggctg
     8581 gtgctcgagc tcgccgacgg cgggagcgcc gttctggtca tctccacgga tctgcccgag
     8641 gtgctgcagt tggccgaccg ggtgctcgtc gtccggcggg gtgccatggt gcgggagttc
     8701 gggcgcggag cgagccaggc ggaggtgctc gccgcagccg cgggcgacaa cggcgacgac
     8761 cccacgtcgg tggccgccga cgcggaagga gcccgcacgt gaccgccacc accggacagg
     8821 tcgatcaggt gcccccggtg acccgccgca ggccccgggt gcgggcgatc gaggggcagg
     8881 aggtcgcgct catcggcgtg accgggctgc tctggctcgt cctctcgctg gccaccgaca
     8941 cgttcctcac ctcgggcaac ctgcagaaca tcctcttcac ggtggcaccg atcgcgctga
     9001 tcggcatcgg tatgaccgca gtgatcgtca cggcgggcat cgacgtctcg gtgggcagcc
     9061 aggccgccgt cgtgatggtc gtcgtcgccc tgctcgtgcg cgacgcgggg ttcgcgttcc
     9121 tgcccgcgct ggccgtggcc gtcgtgagcg ggctggtgct gggcgccctc aacgggctga
     9181 tcgtcgccta cggcggcatc cacccgatca tcgtcacgtt cggcacgctg aacatctacc
     9241 ggttcgtcgc tctgcagatc ttcggcagcg agcaggtcgc cggtgtgccc ggcgcattcg
     9301 caccgatcgg cggtggcgcc gagggtcgcg tgttcggagt gcccgtcgcg tggtggctca
     9361 ccctggtcct ggccgccggc atgtggtgct acatgcggtt ctgggccacc ggccgccacc
     9421 tctacgccgt cggtggcgac gcgttcgccg cccggctggc cggtgtgcgg gtgcggcgac
     9481 ggcagatcag cgcctacgtc ctcaccggcg cctgtgtggg gctcgcggcg tgcgtgctga
     9541 tcggcagcgg ggggctcgtc cagcagaacg tcggcgccgg cctcgagctt cgggtgatcg
     9601 ccgcggtcgt gatcggtggg acgagcatca tcggcggccg cgggaccgta ctgggcaccc
     9661 tgctcggcgc gctgctcgtc ggcaccgtca ccagcgccgt cacgctgctg agctggccca
     9721 ccgagctcac ccagctgttc atcggcgggt tcatcctcgt ggcggtcggc gtcgacctgc
     9781 tgcgcgagcg gcggaggagc gcgcgatgac cgacacccgg gtcccctcgg cacccggggc
     9841 ggcacctccc ggtggtgagg gcctcgcgca gcgcgtgctg cgcctggccc tgaccgagcg
     9901 ggtcgtgctg ctcgcggtgc tgctcgtcgt cgtggtcgtg tggttccgcg tgctgggcga
     9961 cggcgggtac ctcgtcgcgc cctacgacgt gcgctatctc ggtaacgcgc tcgagtcgat
    10021 ggtcccgttc gcgctgctcg cgatcgccga gctgttcgtg atcgtctccg gacgcggggg
    10081 gatcgacctg tcggtcggcg cgatcgtgtc gctcgccggc atggtgttcg ggctgatggt
    10141 cggcgagtgg gggtggccgc tgctcccgtc gatcgtcgcg tgtgtcgcgg tgggcggcct
    10201 cctcggcgct gtcaacgggg cgctcgtggc gtacctgcgg ttccccgcgc tcatcgccac
    10261 gctcgccacg tactacgcgt acagctcgct ggcgctggtg agcaacgcga atgccccgat
    10321 ctccacgccg ccgatcgtgg cgctgcactc cctcacgcgg aatgtgcacc tgccgctgct
    10381 gcccccggtg ccgctgcacg ttttcacgat cctggtcccg tgcgccgtcc tggcctgggt
    10441 ggtcctgtcg cggtccacct acggccgctc gctgtacgcg gtcggcacca acgacatcgc
    10501 cgccggctac gcgacccagc cggtgacgcg cacacggttc accgcctacg tcgtctccgg
    10561 cctgctctcg ggcgtcgtgg cgctggtgac cgtggcgcag ttcgcctcgg cgcgaccgga
    10621 cgccggcgtc gtcggcaacg ggatggccct gcccgccatc acgatcgcgg tgctcggcgg
    10681 cgtcgcgatc cagggcggga tcgggcgggt gggcggcgtg gtcgtcgcgg cattgctcgt
    10741 cacgtggctc aacgcgggca tgctgctcgc gttcgagggg tcggagggca gccagtacca
    10801 actgctcgcg ctggggctcg tcctcgtgct gtcggccctg ctcaacacgc tggccctgcg
    10861 ccgctacgga cggctcgcat gacgttcacc gcccgcccga cggaggtgcc cgatgagctg
    10921 gtcgcgctca cccgccggct cggcgacccc gccgccgacc tggtggtgct cgccgagggc
    10981 aacacgtcgg tccggctcga cgacgaccgg ttcgcggtca aggccagtgg ctcgcggatg
    11041 gacgcggcgg acacggccga cttcgtcgtg ggcgagctgg ccgagctgat cgcggtgctg
    11101 gagaacgggg ccgtcgacca ggacgggctg acgcgcctgc tgtcggccgg gccgacgcgc
    11161 gcgtccatcg agacgctcgt gcacgtggcc gccctcgccg ccggggcaga gtgggtggca
    11221 cacacgcacc ccaccgcggt cgtcgggctg ctggccgtca ccgatgcgga ggcgctgtgg
    11281 tcggcaccgc tgttcccgga cgaggccgtg gtgctcggcg agccggcctg ggtgccatac
    11341 gcgacgccgg gaatcatgct cggacggagc gtgcacgccg cgatccacga gcggctcgac
    11401 cgcgacggcg tcccgccccg gctcgtgctg ctgggcaacc acggcatcgt cgcgctgggc
    11461 gccaccgccg ccgaggtcga gaccgtcacg acgatggcgg tcaaggcggc cagggtgcgc
    11521 gccgtggcgc tgtcggccgg caccccggcc ccgctcgagg tgaggcacgc gcgcgagctc
    11581 gccggccggc ccgacgaggc ggctcggcgc gcactgctcg gacgctcccc gacgagatcc
    11641 caggaggtcc catgaacccg ctcggcatcc acgcgctcgt gtgggcaggc ggctggtccc
    11701 gtgccgagtg cgagcgagcc gtgcgcagca cccgggaggc cgggtacgac ctgatcgaga
    11761 tcccggccct cgaccctgcc gcgatcgacg ccgcgcacac gaaggccgtg ctcgccgagc
    11821 acggcatccg gggtgtctgc tcgctcgggc tcgacctcga cgcggacatc tcctccccct
    11881 accccgacgt cgtgatgcgc ggggaggcga agctcgccga cgcgctggcc gtcgtgacgg
    11941 gtatcggcgg tgactacctg ggcggcgcgg tctactccgc gctggccaag tacgaccggc
    12001 cactcgaccc gcgcgggagg gagaacatgg tggcgggact gcgccggctc gcccgcagcg
    12061 ccgccgacca cggcgtgacg gtggggctcg aacccgtcaa ccgctacgag tccaacgcga
    12121 tcaacacggt cgccgacgcg ctggccgtga tcgaggaggt gggcgaaccg aacgtcgtcg
    12181 tccacctgga cgtctaccac gccaacatcg aggagcggga cttcgtctcg ccggtggtcg
    12241 cggccggcga ccggctcgga tacgtgcacg tgggcgagtc gcaccgcggc tatctgggaa
    12301 ccggcaccat cgatttcccc gccgtcttcg gcgccctcgc cgcagccggc tacaccgggc
    12361 ccgtcacgtt cgagtcgttc tcgcgcgcgg tcgtctcccc cgacctctcc gaccgcctcg
    12421 ccgtctggcg cgacctgtgg tcggacggca tggacctggc cgtccacgcg cgtggcttcg
    12481 tcgccgacgg actggccacg gcgaaggcgc gcgcgtaagg gatcaaaccg cgcggaacgt
    12541 ggccgtcatg gccctgtctt cgtgttccgg cttgtggcag tggaagacgg accgcgcaca
    12601 tctgttggtc accgcgcaca cctcatgacg tgtgtgcggg tgactactgg tgtgcgcttg
    12661 ggcctggcct caaaagcgca catcggacac aagcgtgttc atgaatgcaa gcggtgccca
    12721 atccgcgtgc gcatgcgcct atagagactt ttcgct
//
LOCUS       DASRSC010000363        14874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5231578,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000363 DASRSC010000000
VERSION     DASRSC010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 14874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14874
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5231578"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2482
                     /locus_tag="VFE39_17605"
     CDS             <1..2482
                     /locus_tag="VFE39_17605"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260456.1"
                     /translation="FLVSGENIIRNLELGRARADELGGAMPVGYLPDEFGHCAQMPQI
                     LAAAGFAHACLWRGVPASVDGHAFRWVAPDGSAVRVEYLVGGYGNAASLFADPARFAQ
                     RAAALESQLRPLFGAEPVLGMYGTDHSAPVRSLVGLVAGLAEGGGPVQMQITTLGDYI
                     AGRDAGANGIAVVRGELRSHAAANILPGVLSVRPHLKQAMARAERMVERYAEPLAALW
                     SPTWPQRFLDMAWWRLVDTSGHDSVTGCGVDETAVQVFARISEAEQLGQAVRDRVVGA
                     IGRRVPRDAVVVVNPSATDRTDLVTLDLEVPAEWQAVALEQADGTLLPAQVESSTPAL
                     LHEERMDAAALPTFFPRIHGRELFGLEIVTWAVHADERVLQFDLARHGGEEPFDPDAL
                     RAAVAATAVEGTWTIRLRAQERRVITGLVSAPALGWSSARVVPAPAPAHPSVEQADHA
                     QDRSLDNGLVRVTVHDDGTLRIVAADGTVLDGVGRMVDGGDRGDTYNYAPPATDAVLD
                     APLTVEVTAPDGGPLRSAITVDRSYSWPAGMDWERDRRSDACESASVSMRVELRAGEP
                     FVRLELAIDNRSADHRVRLHVPVARPASASHAEGQLAVVERGLTAEGGDRLGAHSAGT
                     APSGEHPLPTFPAHGFVDAGGAGLLLTQACEYELADGALALTVLRCTGQISRNVHPYR
                     EEPAGPQIPTPQAQMIGTTTVSLAVLPHGGDWVDAGLVGAGERYRHPLHATPGHDLPG
                     GPLESHAGLAAEGAVLTSVRRRDGMLEARLVAQSPDPVTAVLRGHFDAARTVDLRGLP
                     GRDLPVVDGRLELPLRPWEIATVRLGER"
     gene            2494..3234
                     /locus_tag="VFE39_17610"
     CDS             2494..3234
                     /locus_tag="VFE39_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016335517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6260457.1"
                     /translation="MPDRPQVIVDRASPVPLYFQVAHQLEQAIESGELPPGARLDNEI
                     ALAEELGLSRPTMRQAIQYLVDKGLLVRRRGVGTHVARARVRRPVGLTSLFDDLSGSG
                     QQPTTTVLAHSVQPAAGVVAAALEVPEGTMVMSLERLRRTRGEPLAIMHNHLRADLVQ
                     LDPAVLEKRGLYEVLRDAGITLAAAQQSIGARKATGAEARLLDEARGAPMLTMRRTTF
                     DDRGLVVEYGDHLYRASRYSFEVALTGR"
     gene            3459..4736
                     /locus_tag="VFE39_17615"
     CDS             3459..4736
                     /locus_tag="VFE39_17615"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET6260458.1"
                     /translation="MISANAAQGGKNATEADWIVDYVIPEFTKQMAAEGVQATVTFEP
                     SGVDDEQFKTKLSLDMRTGGGADIVNLDGIWVGEFAQAGYIKPLDEVVGEPATTWDGW
                     AQIPDSVEQLASFEGKRYGVPAGTDGRVLFYNKELFAQAGLPANWQPTSWQEVLDAAT
                     ALKKLPGVDPLQLNGGTAMGEATTMQGVLPLLAGTGSVLNTDGKWLGDTPALRDVLGL
                     YQSVYGGGLGDPQLQQEAKGRDKSFQEFAAGRIGILLESDYFWRSVIEPKAGIAPMPN
                     RDQVVGYAKIPAQRPGAGVNGRDFVSMSGGAMRTVNPGTDYPQQAWKLLAFMNSAEAT
                     RALLGDTARITQREDVNRDVLARDPMLSFISTQVLPITAYRPGLAEYPQVSQALQEAT
                     AAVVSGTPPEQALQQYRTALEGALGGTDNITSG"
     gene            4708..5631
                     /locus_tag="VFE39_17620"
     CDS             4708..5631
                     /locus_tag="VFE39_17620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET6260459.1"
                     /translation="MAPTTSPPADLAPPREVATDAAGLGRVRASAFVAPGLALVGVFL
                     VFPALWTLYLGLTNYRLTGIAAASPRFVGLDNYTTALGDPEFLNALWLTLLYVLGSAV
                     IGQTILGFVLAWTMRTVHRTVRTVVESLVMLAWILPSSVVAFLWIAMLDRDSGTLNAL
                     LGTPGMAWLIDHPLLSIVVFNTWRGTAFSMLLFSAALGGVPPSQLETARMAGASGWQQ
                     LRDVVFPHIRGHILTNLLLISLWTFNDFSPYLITAGGPDNRSETLPIFVYQTAIFDGQ
                     LGFGTAISLLMLLINLVLAVVYLRLLRERRS"
     gene            5628..6449
                     /locus_tag="VFE39_17625"
     CDS             5628..6449
                     /locus_tag="VFE39_17625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET6260460.1"
                     /translation="MRVRAVVGRIGFYVLISLITAFMAIPVLWLLFAPFDRTPSLSTS
                     LPEWTWQNFTGLLANPYALTSLGNSVLLALGSMVLVTVLGALAAYALSRVRIPGRDGL
                     LYALLLLSSIVTGTAAMVPIFLLMFELNLIDSQLGVVLVLSGGLLPAAIFILKDFMDG
                     TPRSYEESARVFGAGPLQILRDIVLPVVRPGIATIAVWTVVNGWGNFLTPFILLRSPE
                     KSPAAVVLYTFYTEGGQANLGLISAFSLLYSIPVVAMYVFVNRRYGFRFHGGIKR"
     gene            6449..7585
                     /locus_tag="VFE39_17630"
     CDS             6449..7585
                     /locus_tag="VFE39_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6260461.1"
                     /translation="MADIELRRLVKEYPGGVRAVDGLDLTIADGEFFALLGPSGCGKT
                     TLLRSIAGLETVTSGRIEIGGRDVTRLAPGARDIAMVFQDYALFPHMTVRDNIAYPLR
                     IKKMGKDERHAKAEETAEGLSLGALLDRRPGQLSGGQQQRAALARAVAVRPQVFLFDE
                     PLSNLDARLRLEARTFLKRLQRELGVTTVFVTHDQAEALAMSDRMAVMDAGRIRQIGT
                     PTEVFQRPTSTFVASFIGSTPMNLLDAVVDRDTLRVADVTLPLPAAARPHVRDGEQLV
                     FAARPEYMTFRTEPDPTGLEGVVAIVENMGVGSLVSLEVAGPTDATRIVQVTVPEGQE
                     PEIGSRGWAVAAPERVLLYRRDDGELVGSARSDATDPVLAAAGN"
     gene            7587..8795
                     /locus_tag="VFE39_17635"
     CDS             7587..8795
                     /locus_tag="VFE39_17635"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HET6260462.1"
                     /translation="MSPVAYRGRWTLDDRGEGFYRELPVNVPDGAPALTVRLAFDRRA
                     GVLDLGCFGPAGFRGWSGGARDSFTIAADWATPGYLPGPLEGGTWRVCLGLHRIPPGG
                     LAYEVVVDTAPATAPPVPPALPPPQRPPRRELPAEPGRRWLAGDLHSHTVHSDGALTV
                     DELAHLAAAGGLDFLAVTDHNTVSHHPSLAAAGRRAGVVLLPGQEVTRDAGHANAFGD
                     VGWIDFRRPADDWLTETEQRGGLLSVNHPLANDRAWRHPMARRPRFAEIWHWTWLDRR
                     WGGPLAWWLAWDPTVLPLGGSDFHSPAQGRPLGVPTTWVQCPGGDVTPDDVLAGISAG
                     RIALSAGRDAPVLLRVGDELVVVGGDGTYLVDPHGGRRVVLGDRQSYPARPGPHRLEA
                     PDNALVALVA"
     gene            8846..9064
                     /locus_tag="VFE39_17640"
     CDS             8846..9064
                     /locus_tag="VFE39_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260463.1"
                     /translation="MKQTQQYKLSHMQRTSHTAAAFVATAVVGTVLLGNAPNAEASDD
                     VRGNRGWEASGNRGWSREVRTGSDSDDR"
     gene            complement(9191..10027)
                     /locus_tag="VFE39_17645"
     CDS             complement(9191..10027)
                     /locus_tag="VFE39_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010230350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HET6260464.1"
                     /translation="MDELTGFPARPVVGLLHPGMMGAALGSAMKRAAGAVIWAAAGRS
                     VATSKRAELADLVGVPDVAEVARRSDVIVSICPPHVARAVAEQVAAALGDRAHRPLFV
                     DANAVSPATVQGIGELLGPGRVVDGAVIGPPAWEPGRTVLWLSGAAAAVVAGLFEGSP
                     FEARVLGPDLGAASALKACYALQSKAIPAAWLAVEAAARGYGVQDALRSQLARDGVDL
                     DAKLDDVGARAGAKAWRWVGEMEEAADAVAAVGVPDGFSRSAAEMYRRMAEDRPIDQH
                     KR"
     gene            complement(10111..10914)
                     /locus_tag="VFE39_17650"
     CDS             complement(10111..10914)
                     /locus_tag="VFE39_17650"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HET6260465.1"
                     /translation="MLGVSHATSGAALGLAVAGFAPRWVEMDPSAGTVLTFAAVCAGA
                     ALLPDLDHPSSVATRRFSVASWLACHVVRPLSGFVYDLTKGRRDTGKGTHRGLTHTVA
                     AAVLLGLAVNLASARWGTPVLVGTLFVCLALAIKGLDALVPGPPSLVIAGGLTYAVEE
                     FVPGGTAGTAGWLGTAVALGMVVHSIGDAITESGAPLLWPIRIRRRSWYPVGTPRPLR
                     FRTGGRVEAWVVAPALTVATFLLAMYVVPGVAPLLLDLRAEVERLLGRV"
     gene            complement(11065..12048)
                     /gene="meaB"
                     /locus_tag="VFE39_17655"
     CDS             complement(11065..12048)
                     /gene="meaB"
                     /locus_tag="VFE39_17655"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HET6260466.1"
                     /translation="MAREIDVPALAKGVLGGSRGVLARAITLVESSRADHRQAAQQLL
                     MELLPAAGGARRVGISGVPGVGKSTFIETLGTRLTGAGHRVAVLAVDPSSTRTKGSIL
                     GDKTRMARLATDENAFIRPSPSAGTLGGVARATRETIVLMEAAGYDVVLVETVGVGQS
                     EVAVAAMVDTFLLLTMARTGDSLQGIKKGILELADVVAVNKADGPHEKEATAAARELT
                     GALRLVTPTSASWRPRVLPCSAQTGTGMAEVWAAVEDHRAALEEAGELAGRRADQQVQ
                     WMWRMVRDQLMDRLQADPDVRAAIPQLERDLREGELTPTLAAQAILARLGL"
     gene            complement(12139..14304)
                     /gene="scpA"
                     /locus_tag="VFE39_17660"
     CDS             complement(12139..14304)
                     /gene="scpA"
                     /locus_tag="VFE39_17660"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HET6260467.1"
                     /translation="MSLPDFTRVPLESDAAPAEEWVADVPTWDAPEGIGVRPLYTAAD
                     LADVDFLATYPGIAPYLRGPYPTMYTSQPWTIRQYAGFSTASESNAFYRRNLAAGQKG
                     LSIAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRRLFDGIPLGEMSVSMTMNGA
                     VLPVLALYIVAAEEQGVSHDRLTGTIQNDILKEFMVRTTYIYPPQPSMQIISDIFSFT
                     SREMPRFNSISISGYHIQEAGATNDLELAYTLADGVEYLRAGLDAGLDIDRFAPRLSF
                     FWANGMNFFMEVAKLRAARLLWAKLVAQFSPQNDKSLSLRTHCQTSGWSLTAQDVYNN
                     VIRTCIEAMAATQGHTQSLHTNALDEALALPTDFSARIARNTQLLLQQESGTTKVIDP
                     WGGSYYVERLTYDLARRAWAHIQEVEMAGGMAQAIDAGIPKLRVEEAAARTQARIDSG
                     RQPVIGVNKYVVDQDEDVEVLKVDNAEVRREQLEKLRRLREERDVREVDAALDALTAA
                     AASIAEGSPRTDEQNLLALSVNAARAKATVGEISDALEKVFGRHAGQIRIISGVYSQE
                     AGTSPAIERARELVDEFATEEGRQPRILVAKMGQDGHDRGQKVIATAFADLGFDVDVG
                     PLFQTPGEVARQAVEADVHVVGVNSLAAGHLTLVPELRDELAKLDRPDIMVVVGGVIP
                     PGDYPALRDAGAAAVFGPGTVIAEAAVDLLEKLAAQLRS"
     gene            complement(14375..>14874)
                     /locus_tag="VFE39_17665"
     CDS             complement(14375..>14874)
                     /locus_tag="VFE39_17665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HET6260468.1"
                     /translation="LPRFRLAEPYEAHRDRSDARLAETGARPRAFLATLGPLAAYTAR
                     AGFARNLLQAGGIEVTEAGPTETAQEVGAAFAEAGTPVAVVCSTDRLYGERGAEVVAA
                     LRDAGARYVLLAGRADVPGVDDRLFAGVDALAVIESVYAAQENSAQESSAQESSAQQI
                     SEEER"
BASE COUNT         2070 a         5479 c         5203 g         2122 t
ORIGIN      
        1 gttcctggtc tccggcgaga acatcatccg gaacctggag ctgggccgcg cgcgggccga
       61 tgagctcggc ggggcgatgc cggtggggta cctgcccgac gagttcggcc actgcgcgca
      121 gatgccgcag atcctcgccg ccgcggggtt cgcccacgcc tgcctgtggc gcggcgtccc
      181 cgcgtcggtg gacggtcacg cgttccggtg ggtcgcgccc gacggcagtg cggtgcgagt
      241 ggagtacctg gtgggcgggt acggcaacgc ggcgtcgctc ttcgccgacc cggcccggtt
      301 cgcgcagcgg gccgcggcac tggagtcaca gttgcgtccg ctgttcggcg ccgaaccggt
      361 gctcggtatg tacggcaccg accattcggc cccggtccgc tcgctggtcg gcctcgttgc
      421 ggggctggcg gagggcggcg ggccggtaca gatgcagatc accacgctcg gcgactacat
      481 cgccggccgg gacgccggcg caaacggcat cgcggtcgtc cggggtgagc tgcgcagcca
      541 cgcggcggcc aacatcctgc ccggcgtgct ctcggtgcgc ccgcacctca agcaggccat
      601 ggcccgtgcg gagcggatgg tggagcgcta cgcagaaccg ctcgccgccc tgtggtcgcc
      661 cacgtggccg cagcggttcc tcgacatggc gtggtggcgg ctcgtcgata cgtccgggca
      721 cgactcggtt accggctgcg gcgtggacga gaccgccgtg caggtgttcg cccggatctc
      781 cgaggccgag cagctcggcc aggccgtgcg cgaccgggtg gtgggcgcga tcgggcggcg
      841 cgttccccgg gacgcggtcg tcgtggtcaa cccatcggcg accgaccgca ccgacctcgt
      901 gacgctggac ctggaggtgc ccgccgagtg gcaggccgtg gcgctggagc aggccgacgg
      961 cactctgcta ccggcgcagg tcgagagcag cactcccgcc ctgctgcacg aggagcggat
     1021 ggacgccgcg gcgctaccga cgttcttccc gcgcatccac ggccgcgagc tgttcggcct
     1081 cgagatcgtc acctgggccg tgcacgccga cgagcgcgtg ctgcagttcg acctcgcccg
     1141 ccacggcggt gaagagccct tcgacccgga cgcgctgcgc gccgcggtcg cggccaccgc
     1201 agtggaggga acctggacga tccggctgcg cgcgcaggag cgccgggtga tcaccgggct
     1261 ggtgagcgcg ccggcgctgg gctggagctc ggcacgcgtg gtcccggcac ccgcgcctgc
     1321 ccacccatcc gtcgagcaag cggaccacgc acaggaccgc agcctcgaca acgggctggt
     1381 gcgggtcacc gtccacgacg acgggaccct gcggatcgtc gcggccgacg gcacggtgct
     1441 cgacggggtg ggccggatgg tggacggtgg cgaccgcggt gacacctaca actacgcgcc
     1501 gcccgccacg gacgccgtgc tcgacgcgcc actcaccgtc gaggtcacgg cgccggacgg
     1561 gggcccgctg cgctccgcga tcaccgtcga ccgctcctac tcctggcccg ccgggatgga
     1621 ctgggagcgc gaccggcgca gcgacgcgtg cgagtcggcg agcgtgtcga tgcgggtgga
     1681 gctgcgggcc ggggagccgt tcgtgcggct ggagctcgcg atcgacaacc gcagcgcgga
     1741 ccaccgcgtg cgcctgcacg tcccggtggc gcggccggcg tcggcctccc atgccgaagg
     1801 gcagctcgcg gtggtggagc ggggccttac cgcggagggc ggcgaccgtc tcggcgcgca
     1861 ctcggccggc accgcgccca gcggagagca tccgctgccg acgttcccgg cccacggctt
     1921 cgtcgacgcg ggcggggccg ggctgctgct cacgcaggcc tgcgagtacg agctggccga
     1981 cggtgcgctc gccctgaccg tcctgcgctg cacgggccag atcagccgga acgtgcaccc
     2041 ctaccgcgag gagcccgccg ggccgcagat ccccaccccc caggcgcaga tgatcggcac
     2101 gaccaccgtg tcgctcgcgg tgctcccgca tggcggggac tgggtggacg ccggcctcgt
     2161 gggggcgggc gagcgctacc ggcacccgct gcacgccacc ccgggccatg acctaccggg
     2221 cggcccgttg gagagccacg cgggcctcgc ggcggagggc gccgtgctga ccagcgtgcg
     2281 gcgccgggac ggcatgctcg aggcgcgcct ggtggcgcag tccccggatc cggtcacggc
     2341 cgtactccgc gggcatttcg acgctgctcg gaccgtcgac ctgcgcggtc tgcccggccg
     2401 ggacctcccg gtggtcgacg ggcggctgga gctgccgctg cggccctggg agatcgccac
     2461 cgtccggctc ggagaacggt gaggagcgcg ctgatgcccg accgcccgca ggtcatcgtc
     2521 gaccgcgcaa gccccgtacc cctctacttc caggtcgccc accagctcga gcaggcgatc
     2581 gagtccggcg agctgccccc tggggcgcgc ctcgacaacg agatcgcgct cgccgaggag
     2641 ctcgggctgt cccggcccac gatgcgccag gcgatccagt acctggtgga caagggcctg
     2701 ctggtacgcc gacgcggcgt cggcacgcat gtcgcccggg ccagagtgcg ccgcccggtc
     2761 gggctcacca gcctgttcga cgacctttcc ggctcgggcc agcagcccac gacaacggtg
     2821 ctcgcgcaca gcgtgcagcc ggcggcaggc gtggtcgcgg cagccctgga ggttccggag
     2881 ggcaccatgg tgatgtcgct ggagcggctg cgccgcaccc ggggcgagcc cctcgcgatc
     2941 atgcacaacc acctgcgcgc cgacctcgtg cagctcgacc ctgcggtgct cgagaagcgc
     3001 ggcctgtacg aggtgctgcg cgacgccggg atcaccctcg ccgccgcaca gcagagcatc
     3061 ggtgcccgca aggcgaccgg agcggaggcc cggctgctcg acgaggcccg cggcgcaccg
     3121 atgctcacga tgcgccgcac gacgttcgac gaccggggcc tcgtggtgga gtacggcgat
     3181 catctgtacc gggcctcgcg ctactcgttc gaagtcgcgc ttaccggacg ctgagatcgt
     3241 tcaccccatc gtggacgttg gcccaaggtc atgacatagt cctgtcgtcc tgtcgtgacg
     3301 tactgccaat ggggtgcggg atgtagatca gcaaaggggc tcgggcggca ttcgcgctcg
     3361 cggcgatcac cgggcttacg ctcacgggtt gcggtggcgg cggaccggcg ggcgccggag
     3421 atccccagcg caatgtggac gcgaaaaccg tcaagctcgt gatctctgcg aacgcggcgc
     3481 agggcggcaa gaacgccacc gaggcggact ggatcgtcga ctacgtgatc cccgagttca
     3541 caaagcagat ggcggccgag ggcgtgcagg ccaccgtcac gttcgagccg tctggcgtcg
     3601 acgacgagca gttcaagacg aagctgtcgc tggacatgcg caccggcggc ggggccgaca
     3661 tcgtcaacct cgacggcatc tgggtcggcg agttcgccca ggccggctac atcaagccgc
     3721 tcgacgaggt ggtgggtgaa cccgccacga catgggacgg ctgggcgcag atccccgact
     3781 ccgtcgagca gctggcgtcg ttcgagggga agcgctacgg ggtgcctgcc ggtacggacg
     3841 ggcgcgtgct gttctacaac aaggagctgt tcgcgcaggc cgggctgccc gccaactggc
     3901 agccgaccag ctggcaggag gtgctcgacg ctgccacggc gttgaagaag ctgcccggcg
     3961 tcgacccgct gcagctcaac gggggcaccg cgatgggcga ggccacgacc atgcagggcg
     4021 tcctcccatt gctcgccggc accggttcgg tgctcaacac cgacggcaag tggctgggcg
     4081 acaccccggc tctgcgtgac gtcctcggcc tctaccagtc cgtctacggg ggcggcctcg
     4141 gtgatccgca gctgcaacag gaggcgaagg ggcgcgacaa gtcgttccag gagttcgccg
     4201 ccggacggat cggcatcctg ctggagagcg actacttctg gcgctccgtg atcgagccga
     4261 aggcgggcat cgcgcccatg ccgaaccgcg accaggtggt cgggtacgcc aagatccctg
     4321 cacagcggcc gggcgcgggc gtcaacggac gagacttcgt cagcatgtcc ggcggtgcga
     4381 tgcgcaccgt caaccccggc acggattacc cgcagcaggc ctggaagctg ctggcgttca
     4441 tgaactccgc cgaggcgacc agggcgctcc tgggcgacac ggcccggatc acccagcgcg
     4501 aggacgtcaa cagggacgtg ctggcccgcg atccgatgct cagcttcatc tccacgcagg
     4561 tcctgccgat caccgcgtac cggccggggc tcgcggagta tccgcaggtg tcccaggcgc
     4621 tgcaggaggc aaccgctgcg gtcgtctccg gaacgccgcc ggagcaggcc ctgcagcagt
     4681 accggaccgc gctggagggt gcgctcggtg gcaccgacaa catcacctcc ggctgacctc
     4741 gcgccgccgc gcgaggtcgc gacggatgcc gcgggcctcg gcagggtccg ggcctcggcg
     4801 ttcgtcgcac ccggcctggc gctggtggga gtctttctgg tcttcccggc cctgtggacg
     4861 ctctacctcg ggctcaccaa ctaccggctc accgggatcg ccgctgcgag cccgaggttc
     4921 gtgggcttgg acaactacac gaccgcgctc ggcgatccgg agttcctgaa cgcgctctgg
     4981 ctcacgctgc tctacgtgct gggctccgcg gtcatcgggc agaccatcct cgggttcgtg
     5041 ctggcctgga cgatgcggac cgtccaccgg accgtccgga cggttgtcga gtcgctggtg
     5101 atgctggcgt ggatcctgcc cagctcggtg gtcgcgttcc tctggatcgc gatgctggac
     5161 cgcgactccg gcaccctgaa cgcgctgctg ggcacgcccg ggatggcctg gctcatcgac
     5221 cacccgctgc tctcgatcgt cgtcttcaac acctggcgtg gcacggcgtt ctcgatgctg
     5281 ctgttcagcg ccgcgctggg cggggtgccg ccctcgcagc tggagacggc gcggatggcg
     5341 ggcgcatcgg gatggcagca gctgcgagac gtggtgttcc cgcacatcag gggccacatc
     5401 ctgaccaacc tgctgctgat cagcctctgg acgttcaacg acttcagccc ctacctgatc
     5461 accgcgggcg gccccgacaa ccggtcggag acactcccga tcttcgtcta ccagaccgcg
     5521 atcttcgacg ggcagcttgg cttcggcacg gcgatctcgc tgctgatgct gctgatcaac
     5581 ctggtgctcg cggtcgtcta cctccggctg ctgcgggagc gccgctcatg agggtccgtg
     5641 cggtggtggg gcgcatcggc ttctacgtgc tgatctcgct catcacggcg ttcatggcga
     5701 tcccggtgct gtggctgctc ttcgccccct tcgaccgcac cccctcgctg tccacgtcgc
     5761 tgcccgaatg gacgtggcag aacttcacgg ggctgctcgc caacccctac gccctcacct
     5821 cgctgggtaa ctccgtgctg ctggcgctgg ggtcgatggt gctggtgacg gtgctcggtg
     5881 cgctcgccgc gtacgcactc tcccgggtac ggatccccgg ccgcgacggc ctgctgtacg
     5941 cgctgctgtt gctgtcctcg atcgtgacgg gcacggccgc gatggtgccg atcttcctgc
     6001 tgatgttcga gctgaacctc atcgactccc agctcggcgt ggtgctggtg ctcagcggcg
     6061 gcctgctgcc ggcggcgatc ttcatcctca aggacttcat ggacggcacg ccccgctcct
     6121 atgaggagtc ggcgcgggtg ttcggcgcgg ggccgctgca gatcctgcgc gacatcgtgc
     6181 tgccggtggt gcgccccggc atcgccacca tcgccgtctg gaccgtggtg aacgggtggg
     6241 gcaacttcct cacgccgttc atcctgctgc gcagcccgga gaagtcccct gcggcggtcg
     6301 tgctctacac cttctacacc gagggcggcc aggcgaacct cggcctgatc tcggcgttct
     6361 cgctgctcta ctcgatcccg gtcgtcgcca tgtacgtctt cgtcaaccgg cgttacgggt
     6421 tccgcttcca cggagggatc aagcgctgat ggccgacatc gagctgcgcc ggctggtcaa
     6481 ggagtacccg ggcggcgtcc gcgccgtcga cggcctcgac ctcacgattg ccgacgggga
     6541 gttcttcgcg ctgctcgggc cgtccggctg cggcaagacc acgctgctgc ggtcgatcgc
     6601 cgggctggag acggtgacga gtggccggat cgagatcggc gggcgcgacg tcacccggct
     6661 ggcgccgggg gcgcgggaca tcgccatggt gttccaggac tacgcgctct tcccgcacat
     6721 gacggtgcgc gacaacatcg cctacccact gcggatcaag aagatgggca aggacgagcg
     6781 gcacgccaag gccgaggaga ccgccgaggg actcagcctc ggcgcgttgc tcgaccggcg
     6841 gcccgggcag ctgtcgggcg gccagcagca gcgtgccgcg ctggccaggg cggtggccgt
     6901 ccggccgcag gtcttcctgt tcgacgaacc gctgtccaac ctcgacgccc ggctgcggct
     6961 cgaggcccgc accttcctca agcggctgca gcgcgagctc ggcgtcacca ccgtgttcgt
     7021 cacccacgac caggccgagg cgctggcgat gtccgaccgc atggccgtca tggacgccgg
     7081 ccggatccgc cagatcggca cgccgaccga ggtcttccag cgccccacca gcacgttcgt
     7141 cgcgagcttc atcggatcga cgccgatgaa cctgctcgac gccgtcgtcg accgagacac
     7201 gctgcgggtt gccgacgtga cgctgccgct gcccgctgcc gcccggccgc acgtccgcga
     7261 cggggagcag ctggtgttcg ccgcacggcc ggagtacatg accttccgca ccgagcccga
     7321 tccgaccggt ctggagggcg tggtggccat cgtcgagaac atgggggtcg gctcgctggt
     7381 gtcgctcgag gtggccggcc ccaccgatgc caccaggatc gtccaggtca cggtgccgga
     7441 ggggcaggag ccggagatcg gctcccgggg ctgggccgtc gcggctccgg agcgggtgct
     7501 gctctaccgg cgcgacgacg gcgagctggt cggcagcgcg cggtccgacg cgaccgaccc
     7561 cgtgctcgcc gcggccggga actgacgtgt caccggtcgc gtaccgcggc cggtggaccc
     7621 tcgacgaccg cggcgagggc ttctaccggg agctcccggt gaacgtgccg gacggcgcac
     7681 ccgccctgac cgtccgcctg gcgttcgacc gccgcgcggg cgtgctcgac ctcggttgct
     7741 tcggccccgc ggggttccgc ggctggtccg gcggcgcccg ggactcgttc acgatcgcgg
     7801 cggactgggc gacgccgggc tacctaccgg gtccgctcga gggtgggacg tggcgggtct
     7861 gtctgggact gcaccgcata ccgcccggag gcctcgccta cgaggttgtg gtggacaccg
     7921 caccagccac cgcgccaccc gtcccacccg cgctgccccc tccgcaacgg cctccgcgac
     7981 gcgagctgcc cgcagagcct ggccggcgct ggctcgcggg cgacctgcac agccacacgg
     8041 tccactccga cggcgcgctg accgtcgacg agctggccca cctcgccgcc gccggcgggc
     8101 tcgacttcct ggcggtgacc gaccacaaca ccgtgagcca ccatccctcc ctggccgcgg
     8161 cgggtcgccg ggccggggtc gtgctgcttc ccgggcagga ggtcacccgc gacgccggcc
     8221 acgccaacgc gttcggcgac gtcggatgga tcgacttccg ccgccccgcc gacgactggc
     8281 tgaccgagac cgagcagcgc ggcgggctgc tgtcggtcaa ccacccgctc gcgaacgacc
     8341 gtgcatggag gcacccgatg gcccgcaggc cgcgcttcgc cgagatctgg cactggacgt
     8401 ggctcgaccg gcgctggggc ggcccgctcg cctggtggct cgcctgggac ccgaccgtgc
     8461 tcccgctcgg cggcagcgac ttccacagcc ctgcccaggg ccgcccgctc ggggttccca
     8521 cgacgtgggt gcagtgtccg ggcggtgacg tcacgcccga cgacgtgctg gcaggtatct
     8581 cggcgggacg gatcgcgctg agcgcgggac gtgatgcgcc cgtcctgctc cgcgtcggtg
     8641 acgagctcgt ggtcgtcggc ggcgacggga cctacctcgt cgacccccac ggcggccgtc
     8701 gcgtcgtact cggcgaccgg cagtcgtacc cggcccggcc gggcccgcac cgcctcgaag
     8761 cgcccgacaa cgccctggtc gccctggttg cgtgaggcga acgactcacg gacaccttcg
     8821 agtggaaatg ccgcatccag ctgggatgaa acagacacag cagtacaagc tgtcccacat
     8881 gcaacgcacg tcacacaccg ccgcggcctt cgtcgccaca gcggtggtcg gcaccgtcct
     8941 gctcggcaac gcgccgaatg cggaggcgtc ggacgacgtt cgcggcaacc gaggctggga
     9001 agcatcgggc aaccggggct ggagccggga ggtacgcacc ggatccgatt ccgacgaccg
     9061 ctagccggcc cacgagaccg gggcgttacc cgccccgtcg accgccgccg cccacctccc
     9121 cccggtgcgg cggcggttcg tcgttccgtc ccgtttcccg gatccgtgcc gaccccgacg
     9181 gcgcgcagcc tcagcgtttg tgctggtcta tcgggcggtc ctccgccatc cgccggtaca
     9241 tctcagccgc ggagcgggag aacccgtcgg gcacgccgac cgcggccacc gcgtccgcgg
     9301 cctcctccat ctcccccacc cagcgccacg ccttcgcacc ggcgcgcgcg ccgacgtcgt
     9361 ccagcttggc atcgaggtcg acaccgtcgc gggccagctg ggaacgcagc gcgtcctgca
     9421 cgccgtagcc gcgggccgcc gcctctaccg cgagccaggc agccgggatc gccttgctct
     9481 gcaacgcgta gcaggccttc agcgcgctcg ccgcgcccag gtccggaccg agcaccctgg
     9541 cctcgaacgg cgacccctcg aacaaccccg ccaccaccgc ggcggcggcg ccggacagcc
     9601 acagcaccgt gcgacccggt tcccaggccg gcggcccgat caccgctccg tcgacgacgc
     9661 gaccggggcc caggagctcc ccgatcccct ggaccgtcgc cggcgacacc gcattggcgt
     9721 cgacgaacag ggggcggtgc gcccggtcgc cgagcgcagc tgcgacctgc tccgcgaccg
     9781 ccctcgccac gtgcggcggg cagatcgaga cgatcacgtc ggagcggcga gccacctccg
     9841 cgacgtcggg gacgccgacg aggtcggcga gctcggcgcg cttggaggtg gcgacactgc
     9901 ggcctgcggc cgcccagatc accgcacccg cggctcgctt catcgccgac ccgagcgcgg
     9961 cacccatcat tccgggatgc agcagcccga ccaccggacg ggccgggaac cctgtgagtt
    10021 cgtccacccg accatcatcg accatcgccc ggacggcggt acgacgctcc tcgcgaacat
    10081 ccggatgcgg cacatcacgg gtcgatcagc tcacacgcgg cccaacagcc gttccacctc
    10141 ggctcgcagg tccagcagga gtggcgccac cccgggtacg acgtacatgg ccagcaggaa
    10201 cgtcgccacg gtcagcgcgg gggctaccac ccaagcctcc accctgccgc ccgtgcggaa
    10261 gcgcaacggg cgcggggtgc ccaccggata ccagctgcgg cggcggatcc ggatcggcca
    10321 cagcagcggg gcgcccgact ccgtgatcgc gtcgccgatc gagtgcacga ccatgcccag
    10381 cgccaccgcc gtgccgagcc agccggccgt gcccgccgta ccgccgggca cgaactcctc
    10441 caccgcgtag gtcagcccac ccgcgatcac cagcgacggc gggccgggca ccagcgcgtc
    10501 gagacccttg atcgccaggg cgaggcagac gaagagcgtt ccgacaagca caggggtgcc
    10561 ccaccgcgcc gaggcgagat tcacggccag cccgagcagc acggcggccg ccacggtgtg
    10621 ggtgaggccc cggtgcgtgc ccttcccggt gtcgcgacgt cccttcgtca ggtcgtagac
    10681 gaacccggac aacggccgga cgacatggca ggcgagccag gacgccacgg agaaccgtcg
    10741 ggtcgccacc gacgacgggt ggtccaggtc gggcaggagc gcggcgcccg cgcacaccgc
    10801 ggcgaaggtc agcaccgtcc ccgcgctcgg atccatctcc acccagcgcg gcgcgaaacc
    10861 agctacggcg aggccgaggg ccgcaccgga cgttgcgtga ctgaccccga gcaccggatg
    10921 acgttatcga tcatgcgtgc cctggcatgt gaggcgcgct ggacgagctc ctgatcaagg
    10981 cgcagatgtc gctcgcgcgg cggcacgagc ggcctccgcg ccttgatcga cgccttgcac
    11041 cgtggctgct tgccggcgga gcactcagag acccagacga gccaggatcg cctgcgccgc
    11101 gagggtgggg gtgagctcgc cctcgcggag gtcgcgctcc agctgcggga tcgcggcgcg
    11161 cacgtccggg tcggcctgca gccggtccat cagctggtca cgcaccatcc gccacatcca
    11221 ctgcacctgc tggtcggcac gccggccggc cagctcgccc gcctcctcga gcgccgcgcg
    11281 gtggtcctcc accgcggccc acacctccgc catgccggtg ccggtctgcg cgctgcacgg
    11341 aagcacccgg gggcgccacg acgcactcgt gggcgtcacg agtcgcagag caccggtcag
    11401 ctcgcgggcc gccgccgtgg cctccttctc gtgcgggccg tcggccttgt tgacggcgac
    11461 gacgtcggcc agctccagga tccccttctt gatcccctgc agcgagtcgc cggtgcgggc
    11521 catcgtgagc agcaggaacg tgtcgaccat cgcggcaacg gccacctccg actggcccac
    11581 ccccaccgtc tcgacgagca cgacgtcata gccggccgcc tccatcagca cgatcgtctc
    11641 ccgcgtcgcg cgcgccaccc cgccgagcgt cccggcactg ggcgagggac ggatgaacgc
    11701 gttctcatcg gtcgccagcc gcgccatccg cgtcttgtcg cccaggatcg agcccttcgt
    11761 ccgggtggac gacggatcga ccgcgagcac cgccacccgg tggcccgccc cggtgagacg
    11821 cgtgcccagg gtctcgatga aggtcgactt cccgacgccg ggtacgccgc tgatccccac
    11881 acgccgggct ccgccggctg ccggtagcag ctccatcagc aactgctgcg ccgcctgccg
    11941 gtggtcggca cggctcgact cgaccagggt gatcgcccgc gcgagcaccc cacgcgaccc
    12001 gccgagcacg cccttggcca gggcaggcac atcgatctcg cgggccacgg ccctcctccc
    12061 gagcgtccga ccaccgacat cgacctcagc gtgatccgcc gtgcgcgata gcacgctgcc
    12121 gtcgagctcg atggcaggtc acgacctcag ttgggccgcc agcttctcca gcaggtccac
    12181 ggcggcttcc gcgatcaccg tgcccgggcc gaacaccgcg gcggcccccg cgtcgcgcag
    12241 tgccgggtag tcccccggcg ggatcacccc gcccaccacc accatgatgt cggggcggtc
    12301 gagcttcgcc agctcatcgc gcagctccgg caccagcgtc aggtgtccgg cggcgagcga
    12361 gttgacgccg acgacgtgca cgtcggcctc caccgcctgc cgtgccacct cgccgggggt
    12421 ctggaacagc ggcccgacgt cgacgtcgaa accgagatcc gcgaacgccg tggcgatcac
    12481 cttctggccg cggtcgtgcc cgtcctgacc catcttcgcc acgaggatgc gcggctggcg
    12541 cccctcctcg gttgcgaact cgtccaccaa ctcgcgggcc cgctcgatcg ccgggctcgt
    12601 gccggcctcc tgggagtaca caccggagat gatgcggatc tgaccggcgt ggcgcccgaa
    12661 caccttctcc agcgcgtcgg agatctcccc caccgtggcc ttcgcgcggg cggcgttcac
    12721 cgacagggcg agcaggttct gctcgtcggt gcgcggtgag ccctccgcga tcgaggcggc
    12781 cgcagcggtg agggcgtcga gggcggcgtc gacctcgcgc acgtcgcgct cctcgcggag
    12841 gcgacgcagc ttctccagct gctcgcggcg cacctcggcg ttgtcaacct tcaggacctc
    12901 gacgtcctcg tcctggtcca ccacgtactt gttgacgccg atcaccggct gccgcccgga
    12961 gtcgatgcgc gcctgcgtgc gcgccgccgc ctcctccaca cgcagcttcg ggatgccggc
    13021 gtcgatcgcc tgcgccatcc cgcccgccat ctcgacctcc tggatgtgcg cccacgcgcg
    13081 gcgcgccagg tcataggtca gccgctcgac gtagtagctg ccgccccacg ggtcgatgac
    13141 cttcgtggtg cccgactcct gttgcaggag aagctgtgtg ttgcgtgcga tgcgggccga
    13201 gaagtcggtc ggcagggcga gcgcctcgtc gagcgcgttg gtgtgcaggc tctgggtatg
    13261 gccctgcgtc gcggccatcg cctcgatgca ggtgcggatc acgttgttgt agacgtcctg
    13321 cgcggtcagc gaccagcccg aggtctggca atgggtgcgc aggctgagcg acttgtcgtt
    13381 ctggggcgag aactgcgcca ccagcttcgc ccagagcagg cgtgcggccc gcagcttggc
    13441 gacctccatg aagaagttca tcccgttcgc ccagaagaac gacaggcgcg gggcgaaccg
    13501 gtcgatgtcc aacccggcgt cgagcccggc ccgcaggtac tccaccccgt cggcaagcgt
    13561 gtaggcgagt tcgaggtcgt tggtcgcacc cgcctcctgg atgtggtagc cggagatgga
    13621 gatcgagttg aaccgcggca tctcccgcga ggtgaacgag aagatgtcgg agatgatctg
    13681 catcgacggc tgcggcgggt agatgtaagt ggtgcgaacc atgaactcct tgaggatgtc
    13741 gttctggatg gtccccgtga gcctgtcgtg ggagacgccc tgctcctccg ccgccacgat
    13801 gtagagggcg agcacgggca gcacggcacc gttcatcgtc atcgacaccg acatctcgcc
    13861 cagcgggatg ccgtcgaaga gcctgcgcat gtcgtagatc gagtcgatcg ccacacccgc
    13921 catgccgacg tcgcccgcca cccgcggatg gtcggagtcg tacccccgat gcgtagccag
    13981 gtcgaacgcg atcgacaggc ccttctgccc cgcggcgagg ttgcgccggt agaacgcgtt
    14041 cgactcggac gcggtggaga acccggcgta ctggcggatc gtccagggct ggctcgtgta
    14101 catcgtgggg tacggcccgc gcaggtacgg cgcgatgccc gggtaggtgg cgaggaagtc
    14161 gacgtcggcg aggtcggccg ccgtgtagag cggccgcacg ccgatcccct ccggcgcgtc
    14221 ccacgtcggg acgtcggcga cccactcctc ggcgggggct gcgtcgctct ccaggggcac
    14281 cctcgtgaag tcgggcaggc tcatcgctcg ccctcgctgc gctcgggcgg cgcttcgcgg
    14341 aggcgctgta cccatgattc gctcgcaagc tcgctcatcg ctcctcctcc gaaatctgct
    14401 gggccgaact ctcctgggcc gaactctcct gggccgaatt ctcctgggcc gcgtacaccg
    14461 actcgatcac cgcgagcgcg tccaccccgg cgaagagccg gtcgtccacg ccggggacgt
    14521 cggccctgcc ggcgaggagc acgtaccggg cacccgcatc ccgcagcgcc gccaccacct
    14581 ccgcaccccg ctcgccgtag agcctgtcgg tcgagcacac caccgcaacg ggggtgccgg
    14641 cttcggcgaa cgcggcgccg acctcctggg ccgtctcggt cgggcccgct tccgtgacct
    14701 cgatcccgcc tgcctgcagg aggttgcggg cgaagccggc ccgcgcggtg taggccgcca
    14761 gggggccgag cgtggcgagg aatgcgcggg gccgggcccc ggtctcggcg agccgggcgt
    14821 cggaccggtc gcggtgggcc tcgtagggct cggcgagccg gaaccgcggc aggc
//
LOCUS       DASRSC010000364         5355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1632428,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000364 DASRSC010000000
VERSION     DASRSC010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632428"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFE39_17670"
     CDS             complement(<1..185)
                     /locus_tag="VFE39_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HET6260469.1"
                     /translation="MRIALAQVVSTPDPAHNLGLIEDGARRAAAAGARVVVFPEAAMC
                     CFGVPLGPVAEPLDGPWA"
     gene            217..876
                     /locus_tag="VFE39_17675"
     CDS             217..876
                     /locus_tag="VFE39_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6260470.1"
                     /translation="MQVSPASRESGRDRAYRHLRETVLVDPAVQGTFLNEIELAQDIG
                     VSRTPVREALLLLVADGLVEMVPKRGAYVPPLSGRQIKELMELRGLLERHAASVTLAA
                     GTVPLALMREALAEQERLVDAGSEADTANVFIEWDRRFHQCLVDAARNELIARTYAGL
                     RTRQVRVGVAALFRATDRQHAVCLEHRCILEALESGDEAAAHAAIEDHLRITLQLFLE
                     A"
     gene            complement(928..1743)
                     /locus_tag="VFE39_17680"
                     /pseudo
     CDS             complement(928..1743)
                     /locus_tag="VFE39_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420334.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1914..2603
                     /locus_tag="VFE39_17685"
     CDS             1914..2603
                     /locus_tag="VFE39_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020632129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2848 domain-containing protein"
                     /protein_id="HET6260471.1"
                     /translation="MSELIFDLPDGTTAAVTVIRLLNGGYAGREQEEVAAHVAELTEL
                     GIPAPAVTPALYPVAPYLAAQVDGVPVQHGRTSGEAEWALVVAGEAPGDLLLTVACDH
                     TDRELEVHGVAWSKNAGLDVLGRGAWRLVDVEDHLDDLRLRAWVAQDGGPEQLIQDAP
                     LAALLPPRFWLDELGELLVPGTVLLSGTVAMLPGVDQFADRWRVELADPVTGALLELS
                     YAVERMPEPIG"
     gene            2761..3819
                     /locus_tag="VFE39_17690"
     CDS             2761..3819
                     /locus_tag="VFE39_17690"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005164558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET6260472.1"
                     /translation="MTRTAVPALDEHRTARISPLLSPALLRHELPIDETVAETVTRAR
                     AEVVDILDGRDDRLIVVVGPCSIHDPDAALDYAYRLAEHASRVAGELRIVMRAYFEKP
                     RSTVGWKGLINDPGLDGTFDVNTGLRTARRLLLDVSALGLPVGCEFLDPITPQFLADV
                     VSWGAIGARTAASQVHRQLCSALSMPVGIKNSTDGDVQVAVDGVRAAAASHVFMGINP
                     DGLAALITTAGNPDCHVILRGGSSGPNHDAASVGAALDRLRAVDLPARVIVDASHGNS
                     GKDHVRQAEVVREIAGRIATGEPGVAGVMMESFLVAGRQDLGPSMTRGQSITDACMDW
                     ETTATLLDELAESVAARC"
     gene            complement(3904..4950)
                     /locus_tag="VFE39_17695"
     CDS             complement(3904..4950)
                     /locus_tag="VFE39_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005466212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET6260473.1"
                     /translation="MHILCAGDEFISADLLARALEPALERVGLAGDFIRVDAPWPTEP
                     FTAVDGVREAAGDPRKIAELVRGADALVTHLAPVTAEVLDAGRGSLRVVGVTRGGPVN
                     VDVPAATAAGIPVVHLPGRNLGAVAEFCVGTMICAMRGLPAASAELSAGRWDGAGFRY
                     DRVGLELRAATVGLVGLGAVGLRVAELLRGFGSRLLAHDPYAEPAAADRVGVRLVDLP
                     ELLRESDVVSLHARLTGETTRMIDEAALAAMRPGSVLVNTARGELVDTRALDAALASG
                     HLRAAVLDVFDPEPPSPDDPLPRRPGVLATPHLAGASRQVAEESAERIAAEVARVLTG
                     ARPTACINPETLTA"
     gene            complement(4959..>5355)
                     /locus_tag="VFE39_17700"
     CDS             complement(4959..>5355)
                     /locus_tag="VFE39_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260474.1"
                     /translation="YVTHDYQEALALGDRIGVLREGRLVQVGTPTQVWTEPVNTFVAR
                     RRLGERARLRRGGACPAARRRVQARARPAHRRSPMTVTMDAPPQAQRLRLEALRKTYT
                     ARGRGDGVTLTAASERVHLFDPASGQRLR"
BASE COUNT          789 a         1941 c         1923 g          702 t
ORIGIN      
        1 gcccacggcc cgtccagcgg ctcggccacc ggaccgagcg gtaccccgaa gcagcacatg
       61 gccgcctccg ggaacaccac gacccgtgcc cccgccgccg cggcccggcg tgcgccgtcc
      121 tcgatcaggc cgaggttgtg ggccgggtcg ggtgtcgaca cgacctgggc gagtgcgatc
      181 cgcacaggaa acctccagtt aatgtataca tgatgcatgc aggtatcgcc cgcgtcccga
      241 gagtcggggc gcgaccgtgc ctatcgccac ctgcgggaga ccgtactggt cgatccggcg
      301 gtgcaaggaa ccttcctcaa cgagatcgag ctcgcgcagg acatcggcgt gtcacgcaca
      361 ccggtgcgag aggcgctgtt gctgctggta gccgacggcc tggtcgagat ggtgcccaag
      421 cgcggagcgt acgtgccacc gctgtccggg cggcagatca aggagctgat ggaactgcgg
      481 gggctgctgg aacggcacgc cgcctccgtc actctcgccg caggcacggt gccgctggcg
      541 ctcatgcgcg aggcgctggc cgagcaggag cgactcgtcg acgccggctc cgaggccgac
      601 accgcgaatg tgttcatcga gtgggaccgg cgcttccacc agtgcctcgt cgacgccgca
      661 cgcaacgagc tgatcgcccg cacgtacgcc ggcctgcgga cccgtcaggt acgggtcggg
      721 gtcgcggccc tgttccgggc caccgaccgt cagcacgccg tatgtctgga gcaccggtgc
      781 atcctcgagg cactggaatc cggcgacgag gctgcggccc atgcggcgat cgaggaccac
      841 ctgcggatca ccttgcagct gttcctggag gcgtgaccga agccgcgtat tggacgaggt
      901 aggcctcgtc ctcggccggt cgggattgcg acccaacgcc aacccggcca gcgtcaccag
      961 ccctgccacg aggagataga gcgccaccgg gacccagctc ccccagctgc tgagcagggc
     1021 ggtgaacacg agcggcgcca ccgccccgcc gaagacgccc gcgatcgtgt acgcgagagc
     1081 acctggccgc ggctcatgcc cacgacggtg gtggcgtagg tgagcacgaa gacggtgaag
     1141 agcgcgtagg tgacgtccgg cccgatacgg ctgggtactc cggcgagcag cgcgcggcgc
     1201 tgggtgcgga agacgtcgcg gaccggcgcc gccggctggt cgccccgctc cttgatcgca
     1261 cggaatacgg gcgtgtcctc cagcttcaac cggatccaca gcccgaagcc gaccagcacc
     1321 gcggacagca ggaacgcgat ccgccagccc catgccagga atgcctcgtc cgacatcgag
     1381 agggtgagga cggcgagggc accgttcgag agcagattgc ccgccggtgg cccgagctgc
     1441 gcggcggagg accagaaccc ccgccggcgc gggtcgccga actcgctgga gagcagcacc
     1501 gcaccgcccc actccccacc cacgccgaca ccttgggcga agcgcagcag caccaggatg
     1561 atcggcgcgg ccgcggtcgg agccggggaa gaacagctgc gggaacacca gcgccgcggc
     1621 ggccgagtag acggcgaagt cgtaccactc gagggacgtg ccggtgaggc tcgcgacgaa
     1681 ggccttcgtc agggaacgcc ggttgatctc cggtgtcggt gggccggacg tcgggtcggc
     1741 catcaggtcc tcgtgtcggt gggttgccac actctgggtg gcgcgccgtc atactttcgg
     1801 tatacaaacg gcatgcgcaa gccctgggat gaccggcagt gagcgagctt gcgagcgagg
     1861 cattaacaca gcgccgtccg cgaagcgcgg accgagcgca gcgagggaga gcgatgagcg
     1921 aactgatctt cgatctgccc gacggcacca ccgccgccgt cactgtcatc cggctgctga
     1981 acggcggcta cgcgggccgt gagcaggagg aagtcgcggc gcacgtcgcc gagctgaccg
     2041 agctcgggat ccccgcgccc gcggtgacgc ccgccctcta ccccgtggcg ccctacctgg
     2101 ccgcacaggt cgacggcgtg cccgtgcagc acgggcgcac gtccggcgag gccgagtggg
     2161 cgctcgtcgt cgcgggcgag gcgccggggg acctgctgct caccgtcgcc tgcgaccaca
     2221 ccgaccggga acttgaggtg cacggcgtcg cgtggagcaa gaacgcaggc ctcgacgtgc
     2281 tagggcgcgg cgcgtggcgg ctcgtggacg tcgaggacca tctcgacgac ctgcggctgc
     2341 gtgcgtgggt ggcgcaggac ggcggcccgg agcagctgat ccaggacgcc ccgctggcag
     2401 ccctgctgcc gccccggttc tggctcgatg agctgggtga gctcctggtg cccggcaccg
     2461 tgctgctgtc cggcaccgta gcgatgctgc cgggcgtcga ccagttcgcc gaccgctggc
     2521 gggtggagct cgccgacccg gtcaccggcg cgctgctcga gctctcctac gcggtagagc
     2581 gcatgccgga gcccatcggc tagagtcgat ctcgtgcagc gccagtactg gtttaccgct
     2641 ccggcccgtg acgggtcggc gggcgaaccg ctgcgctgac cttcccccca cgagccggat
     2701 cacgagccgg ctcggtggga tcgtcgacgg ggccggctcg cctgagagag agccgacgtc
     2761 atgacccgca ccgccgtccc cgcgctggac gagcaccgca ccgcccggat cagccctttg
     2821 ctgtcccctg ccctgctgcg ccacgagctc ccgatcgacg agacggtggc cgagaccgtc
     2881 acccgggccc gcgccgaggt cgtggacatc ctcgacggcc gtgacgaccg gctgatcgtc
     2941 gtcgtcggcc cgtgctcgat ccacgacccg gacgccgcgc tcgactacgc ctaccgactt
     3001 gcggagcacg cctcccgcgt cgccggcgag ctgcgcatcg tcatgcgggc ctacttcgag
     3061 aagccgcgct cgacggtcgg ctggaagggc ctgatcaacg accccggcct ggacgggacg
     3121 ttcgacgtca acaccgggct ccggacggca cggcggctgc tgctcgacgt ctcggcgctc
     3181 gggctccccg tcgggtgcga gttcctcgac ccgatcaccc cgcagttcct cgccgacgtc
     3241 gtgagctggg gcgcgatcgg cgcgcggacc gcggccagcc aggtgcaccg gcagctgtgc
     3301 agtgcgctgt cgatgcccgt cgggatcaag aactcgaccg acggcgacgt ccaggtcgcg
     3361 gtggacggcg tccgcgccgc cgccgcgagc cacgtgttca tggggatcaa ccccgacggc
     3421 ctcgccgcgc tcatcacgac ggcgggcaac ccggactgcc acgtcatcct gcgcggcggc
     3481 tcgtccggtc cgaaccacga cgccgcatcg gtcggtgccg ccctggaccg cttgcgggcg
     3541 gtcgacctgc cggcccgggt gatcgtcgac gcgagtcacg gcaacagcgg caaggaccac
     3601 gtccggcagg ccgaggtcgt gcgggagatc gccggccgga tcgcgacggg cgagccgggc
     3661 gtcgccggcg tgatgatgga gagcttcctc gtggccggcc ggcaggacct cgggccgtcg
     3721 atgacccgcg gccagagcat caccgacgcc tgcatggact gggagacgac agcgaccctg
     3781 ctggacgagc tggcagagtc ggtagcggcg cgctgctgag cggcgaggcg tcgtcgcaga
     3841 gacgctgtcg tcatcgcagg gtccacagcc gctgcgagga acacaccacc tctgcgagga
     3901 cgcctaggcc gtcagcgttt ccgggttgat gcaggccgtt ggtcgcgccc ctgtcagtac
     3961 ccgtgctacc tcggcggcta tgcgctccgc cgactcctcc gccacctgcc gagaggcacc
     4021 ggccaggtgg ggtgtggcga gcacgcccgg ccgccggggt agcgggtcgt ccgggctggg
     4081 cggctcgggg tcgaacacgt cgagcaccgc tgcccgcagg tgcccggagg ccagtgccgc
     4141 gtccagggcg cgggtgtcca cgagctcgcc gcgggcggtg ttgacgagca ccgatcccgg
     4201 tcgcatcgcg gcgagtgcgg cctcgtcgat catccgtgtc gtctcgccgg tcagccgggc
     4261 gtgcaggctg acgacgtcgc tctcccgcag cagctcgggc aggtccacca gccggacgcc
     4321 cacgcggtcg gccgcggctg gttcggcgta cgggtcgtgc gcgagcaggc gggagccgaa
     4381 gccgcgcagc agctcggcca cccgcagccc gaccgccccc agcccgacca ggccgacggt
     4441 cgccgcgcgc agctccaggc ccacgcggtc gtagcggaac ccggcgccgt cccagcgccc
     4501 ggcggacagc tcggccgacg ctgccggtag cccccgcatg gcacagatca tcgtgccgac
     4561 gcagaactcg gcgaccgcgc cgagattgcg ccccgggagg tggacgacgg ggatcccggc
     4621 cgccgtggcc gcggggacgt cgacgttcac cgggccaccg cgggtgacgc cgaccacgcg
     4681 gaggctgccc cgacccgcgt cgagcacctc cgccgtcacc ggagcgaggt gggtgaccag
     4741 cgcgtcggca ccgcggacca gctccgcgat ctttcgcggg tcacctgcgg cctcccgcac
     4801 gccgtcgacc gccgtgaacg gctccgtcgg ccatggggca tcgacccgga tgaaatcccc
     4861 agcgagcccc acccgctcca gcgcgggttc gagcgcgcgg gcgagcaggt cggcggagat
     4921 gaactcgtca cctgcacaga ggatgtgcat gctccccgtc accgcagccg ctgcccggac
     4981 gccgggtcga acagatggac gcgctcggac gccgccgtga gagtgacgcc gtcgccgcgg
     5041 ccgcgggcgg tgtacgtctt gcgcagcgcc tccagccgca gccgctgtgc ctgcgggggg
     5101 gcgtccatgg tgacggtcat cgggctcctc ctgtgagctg ggcgggctcg ggcctgcacg
     5161 cggcgccgag ccgcggggca ggctcccccg cgccgaagac gtgcacgttc gcccagtcgg
     5221 cggcgcgcca cgaacgtgtt gaccggctcg gtccatacct gcgtgggcgt cccgacctgt
     5281 accagcctgc cctcgcgcag caccccgatc cggtcgccca gtgcgagcgc ctcctggtag
     5341 tcgtgcgtga cgtac
//
LOCUS       DASRSC010000365         8668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3105251,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000365 DASRSC010000000
VERSION     DASRSC010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 8668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8668
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3105251"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="VFE39_17705"
     CDS             complement(<1..413)
                     /locus_tag="VFE39_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018331895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HET6260475.1"
                     /translation="MTGGFAQVPDGFRSGFACFVGRPNAGKSTLTNALIGSKIAITSS
                     RPQTTRHAIRGIVHRPDAQLVVVDTPGLHKPRTLLGSRLNDVVRETWAEVDVIGFCVP
                     ADAPVGRGDEFIVNELRSVASRTPVLGIVTKTDLA"
     gene            complement(490..1743)
                     /gene="lhgO"
                     /locus_tag="VFE39_17710"
     CDS             complement(490..1743)
                     /gene="lhgO"
                     /locus_tag="VFE39_17710"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HET6260476.1"
                     /translation="MTFPVTPKFDVVVVGGGIVGLATAHAVARTGRSVAVVEREQRLG
                     THQTGHNSNVIHSGLYYAPGGLKARLAVAGCAETVAFCREHDLPHEVTGKLVVATEPE
                     ELPRMAELARRGAANGVPVHELDQAGMRQHEPHVRGIAALHVPSTGICDYRLVAEKIG
                     ELVAKEGGEIHVGRSVLRLVRRREDVLVRSDGTDLLAKQVVVCAGLRCDELAKASGAD
                     PGIRIIPFRGEYVGFSDRAAALVKGLIYPVPDPAFPFLGVHATRGIDGHVHAGPNAVL
                     ALAREGYSWGTIKPKELAQSLAYPGMLRLARRHWKYGLGEVHRSLSKKAMVQQIQRML
                     PDVQAEDLSPAGAGVRAQAVKPDGSLVDDFLFVGQPGLQGERGPGSILHVLNAPSPAA
                     TAALPIGREILERLTGERIAPLSAT"
     gene            complement(1740..2126)
                     /locus_tag="VFE39_17715"
     CDS             complement(1740..2126)
                     /locus_tag="VFE39_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET6260477.1"
                     /translation="MSDQGDLAPEALDPEDAKIVTLARSVRARTGAAEGAAVRDTDGR
                     TYAATSVALPSLSLSALQAAVAAAVSSGAPGLEAAAVVTSSDTVDAPSLAAVHDLAPT
                     AHVLRADPTGAVRQIHSPQPTTETSA"
     gene            complement(2110..3504)
                     /locus_tag="VFE39_17720"
     CDS             complement(2110..3504)
                     /locus_tag="VFE39_17720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010226185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HET6260478.1"
                     /translation="MTLPLIVIAVLLVPLAGLFAAADAALNSVSRARVDALVRAGRPG
                     SRALATVVADRPRHVNLLLLLRLAAETAATVLLTVALMSSIKPPWVAALTAGAIMVVV
                     SYVLIGVGPRTLGRQHPYRVGMLVAAPIRALATLLGPLTKLLIVVGNAITPGQGFRQG
                     PFSSEVELRELVDMASTSGVVDEDERQMIHSVFELGDTLVREVMVPRPDVVWTERDTP
                     VEKVLRLALKSGYSRIPVLGESIDDIVGVAYLKDLVQAVQSGDPADDPAADGQVAALP
                     SVMRAAVFVPDSKRIDELLKEMQRTRNHMAIVVDEYGGTAGVVTIEDILEEIVGEITD
                     EYDSEEVPDVQELDGRRLRDSARLPVEDLVELFPGVVGVEGELAEVLEAADVDTVGGL
                     LAQQLGRVPLPGARAEIPGLRLLAEGGKDARGRIRINTVLVEPVDPAADDEERDGAGA
                     ERTDRREESNVRPG"
     gene            complement(3553..4089)
                     /gene="ybeY"
                     /locus_tag="VFE39_17725"
     CDS             complement(3553..4089)
                     /gene="ybeY"
                     /locus_tag="VFE39_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET6260479.1"
                     /translation="MSIEVCNESGVTVDESLIASVARFALDAMHVNPAAELSILAVTT
                     EVMSELHERWMEEPGPTDVMSFPMDELTEESRRPDAPEMGPALLGDIVLCPEFATAQA
                     RTAGHALADELHLLTVHGVLHLLGYDHAEPDEEREMFALQSKLLADWRTAAAEAAARE
                     AQRRSDSAVLGTVGLEDS"
     gene            4161..5408
                     /locus_tag="VFE39_17730"
     CDS             4161..5408
                     /locus_tag="VFE39_17730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020658546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HET6260480.1"
                     /translation="MVTVQPSTNWAGNIVFGARRFHRPRSVPQLQELVATGDRIRALG
                     TGHSFSRIADTTGDLVSLADLPPVTRIDADAATVTVSAGMRYGEVTGQLHAAGFALPN
                     LGSLPHISIAGACSTGTHGSGDGNGALATAVSALELVGPDGELRAVRRGDADFPGSVV
                     ALGALGIVTALTLDLRPTFGLRQWVYEGLRGFDDVEEVLASAYSVSLFTYWTGAGFDQ
                     VWLKQRDTDPERPQRWLGADRADGQRHMVRGVDPAHCTPQLGVAGPWHARLPHFRLEF
                     TPSSGDELQSEYLLPRETLVAALWALHRIRDTIAPVLQVCEVRSVAADDLWLSLAHDR
                     DSIALHFTWVADTPAVLPVVAAIEAALKPFAARPHWGKVFGTDPDTVRALWPRLPDVE
                     RLVRAHDPAGKFRNDMLDLYLPA"
     gene            complement(5486..7069)
                     /locus_tag="VFE39_17735"
     CDS             complement(5486..7069)
                     /locus_tag="VFE39_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6260481.1"
                     /translation="MKLSRRAVVASTIALLVALSVSAPAAMAAPVPAQAGLDRFLNQQ
                     LSWGPCADFARSDADRQAYADPKFDCTYLEVPLDYAQPDGKVARIAMLRQKALDPAAR
                     IGSLFTDPGGPGGSGVSFLPALAGLIGTGEVAQKFDLIGFDPRGVGVSEPTIDCSNTA
                     EIDAERADLDVDTAPQGVAQTEAEYKAFAQRCADRVGLDVLANVGTRDVARDLNIAHR
                     VVGDEKLSYVGYSYGTQIGAEYAEQFPGSVRALVLDGAVDPSQNPVESRLTQSRGFQR
                     AFDNFATACVARAGCPLGTDVPGASRKLQGLLKPLITAPIAAPGGRTLSYNDAMTGVV
                     TALYSESLWGFLLDGLVQVTQGNGDNLLRLADAYYERRPDGSYSNLLEAFPVVHCADS
                     DRLTDRQVALDISRQSLQIAPFFDAGFGPSNALEQCSFWPVPPTSEPGPVEAPGLPTV
                     LVVSSTGDPATPYEDGVSLAKQMQARLLTVENDGHTVALQGANPCADTTATRYLVDLT
                     LPGGDTTCARPAPPAPAGG"
     gene            complement(7129..8154)
                     /locus_tag="VFE39_17740"
     CDS             complement(7129..8154)
                     /locus_tag="VFE39_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET6260482.1"
                     /translation="MAVPDAALLALLGSRDESLRVAEDMLEADVHVRGNELTLSGAPA
                     DVAFAERVFTELIMLAERGQQVSTDAVRRTVEMLADGEADERARIGESPAEVLSLDIL
                     SRRGKTIRPKTLNQKRYVDAIDAHTIVFGIGPAGTGKTYLAMAKAVQALQAKMVNRII
                     LTRPAVEAGERLGYLPGTLYEKIDPYLRPLYDALHDMIDPESIPKLIAAGTIEVAPLA
                     YMRGRTLNDAFIILDEAQNTTPEQMKMFLTRLGFGSKIVVTGDVTQVDLPGSTRSGLT
                     VVRDILGDVDDVHFSLLTSTDVVRHRLVADIVDAYARWDAGNERPNLRAAAPRTGPPQ
                     DRRTRRR"
     gene            8408..>8668
                     /locus_tag="VFE39_17745"
     CDS             8408..>8668
                     /locus_tag="VFE39_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260483.1"
                     /translation="MHDDSALVVPRIARFAQERLGPALYRDRVPVQVTAWAAPGEPVP
                     FAAAVQQEFRRFEVGSVWGRPWGTVWFHVTGSVPQAWTDSAAR"
BASE COUNT         1207 a         3103 c         3104 g         1254 t
ORIGIN      
        1 tgcgcgaggt cggtcttggt gacgatcccg agcaccggtg tgcggctggc gaccgagcgc
       61 agttcgttga cgatgaactc gtcgcccctg cccacgggcg cgtcggccgg cacgcagaaa
      121 ccgatcacgt cgacctccgc ccaggtctcg cggaccacgt cgttgagcct gctgccgagc
      181 agcgtgcgcg gcttgtgcag gcccggggtg tcgaccacca cgagctgcgc atcgggccgg
      241 tgcacgatcc cgcggatggc atggcgcgtg gtctgcggcc ggctggacgt gatcgcgatc
      301 ttcgacccga tgagcgcgtt ggtcagcgtc gacttacccg cgttgggccg cccgacgaag
      361 caggcgaacc cggaccggaa gccgtccggg acctgggcga acccgccggt cacgccgcac
      421 cccaccctgg tcgtgcgcgg aaacgggtgc tctggcactc gtatccgcgc acgggcgccg
      481 caggcccggt cacgtcgcgc tcagcggagc gatccgctcg ccggtgaggc gctccaggat
      541 ctcgcgcccg ataggcaatg cagcggtagc ggcaggagaa ggcgcgttga gaacgtgcag
      601 aatggagccg ggcccgcgct cgccctggag accgggctgc ccgacgaaca ggaagtcgtc
      661 caccagcgag ccgtccggct tcacggcctg cgcgcgcacc cccgcgccgg cgggcgagag
      721 gtcctcggcc tgcacgtccg gcagcatccg ctggatctgc tgaaccatgg ccttcttcga
      781 cagcgagcgg tgcacctcgc ccagcccgta cttccagtgc cgccgggcca gccgcagcat
      841 gccggggtag gccagcgact gcgcgagctc cttcggcttg atcgtgcccc acgagtagcc
      901 ctcgcgcgcc agggccagca ccgcgttcgg accggcgtgg acgtgcccgt cgatcccccg
      961 ggtggcgtgc acgccgagga aggggaacgc cgggtcgggc accgggtaga tcaggccctt
     1021 gacgagcgcg gcggcccggt cgctgaaccc gacgtactcg ccacggaacg ggatgatccg
     1081 gatcccgggg tcggcaccgg aggccttcgc cagctcgtcg cagcgcagcc cggcgcagac
     1141 caccacctgc ttggcgagca ggtcggtgcc gtcgctgcgc accagcacgt cctcgcggcg
     1201 gcgcaccagc cgcagcaccg agcggccgac gtggatctcg ccaccctcct tcgcgaccag
     1261 ctccccgatc ttctccgcca ccagccggta gtcgcagatg ccggtggacg gcacgtggag
     1321 cgccgcgatg ccgcggacgt gcggctcgtg ctgccgcatc ccggcctggt cgagctcgtg
     1381 caccggcacg ccgttggcgg cgccgcggcg ggccagctcg gccatccgcg gcagctcctc
     1441 gggctcggtg gccacgacga gcttgcccgt gacctcgtgc ggcaggtcgt gctcccggca
     1501 gaatgcgacg gtctccgcgc agccggccac cgcgagccgc gccttgaggc cgccgggcgc
     1561 gtagtagagc ccggagtgga tcacgttgga attgtggccg gtctgatgtg tgccgagccg
     1621 ctgctcgcgc tccacgacgg cgaccgagcg accggtgcgg gccacggcgt gggcagtggc
     1681 caggccaacg atgccgccgc cgaccacgac gacgtcgaac ttcggggtga cggggaaggt
     1741 catgcggacg tctccgtggt gggctgaggt gagtggatct gccggacggc cccggtgggg
     1801 tcggcgcgca ggacgtgggc ggtgggagcg aggtcgtgca cggcggcgag tgaaggggcg
     1861 tccaccgtgt cggacgaggt gacgaccgcg gcggcctcga gcccaggagc accgctggac
     1921 accgcggcgg cgacggcggc ctgcagcgca ctgagcgaca gcgacgggag cgcgacggac
     1981 gtggccgcgt aggtgcggcc gtcggtatcg cggacggccg caccctcggc cgcgcctgtg
     2041 cgcgcgcgga ccgagcgggc cagcgtcacg atcttggcgt cctccgggtc gagggcctcg
     2101 ggggccaggt caccctggtc ggacattgct ctcctcccga cggtcggtgc gctcagcgcc
     2161 cgcgccgtcc cgttcctcgt cgtccgcggc ggggtcgacc ggctcgacaa gcaccgtgtt
     2221 gatccggatc cggccgcggg cgtccttgcc accctccgcc agcagccgca gcccgggaat
     2281 ctcggcccgc gcgcccggca gcggcacccg gccgagctgc tgggcgagca ggccgcccac
     2341 ggtgtcgacg tcggcggcct ccagcacctc cgcgagctcc ccctcgacgc cgaccacccc
     2401 cgggaacagc tccacgaggt cctccaccgg caggcgtgca gagtcccgga gcctacggcc
     2461 gtccagctcc tggacgtcgg gcacctcctc ggagtcgtac tcgtcggtga tctcgccgac
     2521 gatctcctcg aggatgtcct cgatcgtcac gacacccgcg gtgccgccgt actcgtcgac
     2581 gacgatcgcc atgtggttgc gggtgcgctg catctccttg agcagctcgt cgatgcgctt
     2641 gctgtcgggc acgaacaccg cggcccgcat caccgagggc aacgcggcca cctgcccgtc
     2701 ggccgcggga tcgtcagccg ggtcgccgga ctgcactgct tgaaccaggt ccttgaggta
     2761 cgcgacgccc acgatgtcgt cgatgctctc gccgagcacc gggatgcgcg agtacccgct
     2821 cttcaaggcc aaccgcagca ccttctcgac cggggtgtcg cgttcggtcc acaccacgtc
     2881 cgggcgcggc accatcacct cgcgcacgag cgtgtcgccc agctcgaaca ccgagtggat
     2941 catctgccgc tcgtcctcgt cgaccacgcc gctggtgctc gccatgtcga ccagctcgcg
     3001 cagctccacc tcggaggaga acgggccctg ccggaagccc tgcccggggg tgatcgcgtt
     3061 accgaccacg atcagcaact tcgtgagcgg gcccaacagc gtggcgagcg cccggatcgg
     3121 cgccgcgacc agcatcccga cgcggtaggg gtgctggcga ccaagggtgc ggggcccgac
     3181 cccgatgagc acgtagctca ccacgaccat gatcgcaccg gcggtgagcg cagcgaccca
     3241 cggcggcttg atgctgctca tcaacgccac cgtgagcagg acggtagcgg cggtctcggc
     3301 ggcgagccgc agcagcagga gcaggttgac gtgccgtggt cgatcggcca cgacggtggc
     3361 gagcgcccgc gacccggggc gccccgcccg cacgagcgcg tcgacgcggg cccgggagac
     3421 cgagttgagc gcggcgtcgg cagcggcgaa caggcccgcc agcgggacga gcagcaccgc
     3481 gatcacgatc aacgggaggg tcatgacgct cgttccgacg agcttggcgc ggtgccgtcc
     3541 atgacgtgcc ggtcagctgt cctcgagccc gacggtaccc agcaccgcgg agtcgctccg
     3601 gcgctgcgcc tcgcgcgcgg cggcctccgc agcggcggtg cgccagtcgg ccaggagctt
     3661 gctctggagg gcgaacatct cgcgctcctc gtcgggctcc gcgtggtcgt agccgagcag
     3721 gtgcagcacg ccgtgcacgg tgagcaggtg cagctcgtcg gcgagcgcgt ggcccgccgt
     3781 gcgggcctgc gcggtcgcga actccgggca gagcacgatg tcgccgagca gcgccggccc
     3841 catctccggc gcatccgggc ggcgggactc ctcggtgagc tcgtccatgg gaaacgacat
     3901 gacgtcggtg gggcccggct cctccatcca gcgctcgtgc agctcgctca tgacctccgt
     3961 ggtgacggcc aggatactca gctcggcagc gggattgacg tgcatcgcgt ccagggcgaa
     4021 acgcgccacc gaggcgatga gcgactcgtc gacggtgacg ccggactcgt tgcagacctc
     4081 gatcgacacc tgttacccct gatccgccgg gtcgtgaaca gtggccagcg tagaccgtgg
     4141 ccctctgtag gttcgcctgc atggtcaccg tgcagccgtc gacgaactgg gcaggcaaca
     4201 tcgtcttcgg agcccgccgc ttccaccgac cgcgttcggt gccgcaactc caggagctgg
     4261 tggcaaccgg cgaccggatc cgggcgctcg gcaccggcca ctcgttcagc cgaatcgccg
     4321 acaccaccgg cgatctggtg tcgctcgccg acctcccacc cgtcacacgc atcgacgcgg
     4381 acgccgccac cgtcacggtg tcggcaggca tgcgctacgg cgaggtgacc gggcagctgc
     4441 acgcggccgg gttcgccctg cccaacctcg gctccctgcc gcacatctcc atcgcgggcg
     4501 cctgctcgac cggcacccac ggctccggcg acgggaacgg ggcactcgcg acggccgtgt
     4561 ccgccctcga gctggtgggc cccgacggcg agctgcgcgc cgtgcgccgc ggggacgccg
     4621 acttccccgg ctcggtggtc gccctcggcg cgctggggat cgtcaccgca ctgacgctcg
     4681 acctgcggcc cacgttcggc ctccggcagt gggtgtacga gggactgcgc gggttcgacg
     4741 acgtcgagga ggtgctcgcg tccgcctaca gcgtcagcct cttcacctac tggaccggcg
     4801 cgggcttcga ccaggtctgg ctcaagcagc gcgacaccga ccccgagcgc ccgcagcgct
     4861 ggctgggtgc cgaccgcgcc gacgggcagc ggcacatggt gcggggcgtc gacccggccc
     4921 actgcacgcc acagctcggc gtagcgggcc cgtggcacgc gcggctgccg cacttccggc
     4981 tcgagttcac cccgagtagc ggtgacgagt tgcagtcgga gtacctgcta ccgcgggaga
     5041 cgctcgtcgc cgcgttgtgg gccctgcacc ggatccgcga cacgatcgcg cccgtcctgc
     5101 aggtctgcga ggtccgctcg gttgccgccg acgacctgtg gctctctctc gcacacgacc
     5161 gcgacagcat cgccctgcac ttcacctggg tcgccgacac ccccgccgtg ctcccggtgg
     5221 tggccgccat cgaggccgcg ctgaaaccgt tcgccgcccg gccgcactgg ggcaaggtct
     5281 tcggcaccga cccggacacc gtccgtgcgc tctggccgcg cctccccgac gtcgagcgcc
     5341 tggtgcgggc ccacgacccg gccggcaagt tccgcaacga catgctcgac ctctacctac
     5401 cggcctgacg ccggctcgcg ctggcaccag agcgtgtcgt gcgcggatac gagtgcttca
     5461 gcactcgtat ccgcgcacag gggcgtcagc cgcccgcggg cgcgggtggc gcgggacggg
     5521 cgcaggtggt gtctcccccc gggagggtga ggtcgacgag gtaacgcgtg gcggtggtgt
     5581 cggcgcacgg gttggccccc tgcagggcga cggtgtgccc gtcgttctcc actgtcagca
     5641 gccgggcctg catctgcttc gccagcgaca cgccgtcctc gtacggggtg gccgggtcgc
     5701 cggtcgacga gacgaccagc accgtgggca ggccgggtgc ctcgacggga ccgggctcgc
     5761 tggtcggcgg gaccggccag aacgagcact gctcgagcgc gttggacggc ccgaacccgg
     5821 cgtcgaagaa cggggcgatc tgcagcgact gccggctgat gtcgagcgcg acctgacgat
     5881 cggtgagccg gtccgagtcg gcgcagtgga ccaccgggaa ggcctcgagg aggttgctgt
     5941 agctgccgtc ggggcgccgc tcgtagtagg cgtcggccag ccgcaggagg ttgtcgccgt
     6001 tgccctgggt cacctgcacg agaccgtcca gcaggaagcc ccacagcgat tcggagtaca
     6061 gcgcggtgac gaccccggtc atggcgtcgt tgtagctcag cgtccggccc cccggcgccg
     6121 cgatcggtgc cgtgatgagc ggcttcagca gtccctgcag cttgcgcgac gcgcccggta
     6181 cgtccgtacc cagcgggcat cccgctcgcg ccacgcaggc cgtggcgaag ttgtcgaacg
     6241 cccgctggaa gccacgcgac tgggtcagcc gcgactccac cggattctgc gacgggtcga
     6301 ccgcgccgtc gagcacgagt gcacgcacgg agccggggaa ctgctcggcg tactccgcgc
     6361 cgatctgggt gccgtaggag tagccgacgt agctcagctt ctcgtcgccc accacccggt
     6421 gggcgatatt caggtcgcgg gccacgtcac gggtgccgac gttggcgagc acgtcgaggc
     6481 cgacccggtc cgcgcaccgc tgcgcgaagg ccttgtactc ggcctcggtc tgcgccacgc
     6541 cctgcggcgc cgtgtcgacg tcgagatccg cgcgttcagc gtcgatctcc gcggtgttcg
     6601 agcagtcgat cgtcggctcc gacacgccga cgccgcgtgg gtcgaacccg atgaggtcga
     6661 acttctgggc cacctcgccg gtgccgatca ggccggcgag ggccggcagg aagctgacgc
     6721 ccgaaccgcc ggggccaccg ggatcggtga acagcgagcc gatccgcgcc gccgggtcga
     6781 gcgccttctg ccggagcatc gcgatccggg cgacctttcc gtcgggctgc gcgtagtcga
     6841 gcggcacctc gaggtacgtg cagtcgaact tcgggtcggc gtaggcctgt cggtcggcgt
     6901 cgctacgcgc gaagtcggcg caggggcccc aggacagctg ctggttcaga aaccgatcga
     6961 gtcctgcctg cgcgggcacc ggtgccgcca tggctgcggg cgcagagacg gacaaggcga
     7021 ccagaagggc gattgtgctc gcgacgaccg cgcgacggga caacttcacg aacgggatcc
     7081 tgacggtgtt ctgcctcccc ggcaaccacc tgggccatgc cgtgtcgatc agcgccgacg
     7141 cgtccgccgg tcctgcggtg ggcccgtgcg cggagcggct gcccgcaggt tcggtcgctc
     7201 gttgccggcg tcccagcgcg cgtaggcgtc gacgatgtcg gccaccagcc ggtgccgcac
     7261 gacgtcggtg ctcgtgagca gggagaagtg cacatcgtcg acgtcgccga ggatgtcgcg
     7321 caccacggtg agcccggaac gggtgctacc gggcaggtcc acctgcgtga cgtcaccggt
     7381 cactacgatc ttggagccga acccgagccg cgtcaggaac atcttcatct gttcgggcgt
     7441 ggtgttctgg gcctcgtcaa gaatgatgaa cgcgtcgttg agcgtgcgcc cgcgcatgta
     7501 cgccagtggc gccacctcga tcgtgccggc agcgatcagc ttcgggatcg actcggggtc
     7561 gatcatgtcg tgcagcgcgt cgtagagcgg gcgcaggtac gggtcgatct tctcgtagag
     7621 cgtgccgggc aggtacccga gccgctcccc cgcctcgacg gccggtcggg tgaggatgat
     7681 ccggttgacc atcttggcct gcagcgcctg tacagctttg gccatcgcga ggtaggtctt
     7741 gccggtacct gcggggccga tgccgaagac gatcgtgtgc gcgtcgatcg cgtcgacgta
     7801 gcgcttctgg ttgagggtct tgggccggat cgtcttgccg cgccgggaca ggatgtccag
     7861 gctcagcacc tcggccggcg actccccgat gcgagcacgc tcatcggcct cgccgtcggc
     7921 gagcatctcg acggtgcgcc gcaccgcgtc ggtggacacc tgctggccac gctcggcgag
     7981 catgatcaac tcggtgaaga cacgctccgc gaacgccacg tcggccgggg cgccggacag
     8041 ggtgagctcg ttgccacgca catggacgtc ggcctcgagc atatcctcgg cgacgcgcag
     8101 gctctcgtcg cgcgaaccga gcagcgcgag cagagcggcg tccggaacgg ccatgcggga
     8161 ctggacgggg tccggatgtg acaagggctg tgccgcctgc tttccatgtc tctgctggct
     8221 ctggaccctc cgatgctacc gccaccagcg ggtggtgcgc ccgggctttc ggcggcaagt
     8281 actgctcgcc cgggccgggt gaggtccggc gccaaccagc gatgcgcttc cggctctgcc
     8341 gtaccgagcc cgccgcaacc cgcttcccgt tttcggcgca gatgacgaag ccagccagga
     8401 tggacgggtg cacgatgact ccgccctcgt cgtcccgcgt atcgcccgct tcgcgcagga
     8461 gcggctcggc cccgctctgt accgggaccg ggtcccggtg caggtgacag cctgggcggc
     8521 gccgggcgag ccggtgccgt tcgcggcggc cgtgcaacag gagttccggc ggttcgaggt
     8581 cgggtcggtg tggggcaggc cgtggggcac ggtgtggttc cacgtcacgg ggtcggtccc
     8641 gcaggcatgg acggattccg ccgcgcgg
//
LOCUS       DASRSC010000366        18182 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2287619,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000366 DASRSC010000000
VERSION     DASRSC010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 18182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18182
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2287619"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VFE39_17750"
     CDS             <1..303
                     /locus_tag="VFE39_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET6260484.1"
                     /translation="PALVRSGSPPALLLGARGGRLDPRVARTVVHRALAAVPGAPDVG
                     PHGLRHAAATHMLEGGADLRYVQELLGHAKLATTQLYTHVTVERLKVVHEQAHPRA"
     gene            278..1159
                     /locus_tag="VFE39_17755"
     CDS             278..1159
                     /locus_tag="VFE39_17755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010305995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HET6260485.1"
                     /translation="MSRRTRGRDVVLVESVQLRAMTGPVVTVPVAPSADPVVPVVPVV
                     DTADPDAVYEVWTAYLDQPTRQLRDRLVLHYTPLVRSVAHRVAAGLPSYVERADLVQC
                     GIFGLIDAIERFDPQRCPRFESYAGQRIRGAIFDELRAQDWVPRSVRGRVRDLERAQE
                     RLESRLLRTATDRELAQELGVPVHELRMLTRQVQLISVEALEESSGGVSELFADDAAP
                     DPMVVVQSHETLRQLSVAVEQLGERDRTVVRLYYLENRTLAEIGSLLGVTESRVCQLH
                     SRLVGRLRGRLEELAAG"
     gene            complement(1233..2060)
                     /locus_tag="VFE39_17760"
     CDS             complement(1233..2060)
                     /locus_tag="VFE39_17760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HET6260486.1"
                     /translation="MALERRQGRLRLRPRAGRPLRAALTAGLLCAVTAALGGMGPSSL
                     AGSSGAGQKGASQNEAASGEARRGNTGPRNTGPDNTGPDKAVVFTSVRGPAHLTALAP
                     PVPGPPPADEERPELVAPAPPPEAGVSAPGARYAWPLVPAPAVVTPFRAPAHPYGPGH
                     RGVDLAGAPLQPIVAARGGTVVFAGPVGGRDLVSVQHDDGLRTTYEPLQPTVRAGAIV
                     LAGEVIGLLQPGHAGCATPACLHWGVRRDRADYLDPLVLLRPLRVRLLPVPVPWPDG"
     gene            2410..3309
                     /gene="rpsB"
                     /locus_tag="VFE39_17765"
     CDS             2410..3309
                     /gene="rpsB"
                     /locus_tag="VFE39_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET6260487.1"
                     /translation="MAVVTMKQLLDSGVHFGHQTRRWNPKMKRYILTERNGIYIIDLQ
                     QTLSYIDRAYEFVRETVAHGGTIMFVGTKKQAQEAIADEAGRVNMPYVNQRWLGGMLT
                     NFQTVHKRLQRMKELESLEQTGGFEGRTKKEILMLTREKTKLEKTLGGIRDMTKVPSA
                     VWIVDTKKEHIAVGEARKLGIPVVSILDTNCDPDEVDFPIPGNDDAIRSAALLTKVIA
                     RAAADGLMARSARSRGDGDEKPEAGVGSDEPLTEWEQNLLTNGAGSDGASLTAAAPAT
                     EAPNPAAEPAPASTSNGTQQTAG"
     gene            3408..4226
                     /gene="tsf"
                     /locus_tag="VFE39_17770"
     CDS             3408..4226
                     /gene="tsf"
                     /locus_tag="VFE39_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009074497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HET6260488.1"
                     /translation="MANYTAAEVKKLRELTGAGMMDCKNALEQADGNIEQAVEALREK
                     SAKSVDKRTERTTTNGLVAASGGTMIELNSETDFVAKSADFIQLAETILQVAVEQQPA
                     DVEALNAAPLGDGTVLDAVQALSGRIGEKLQIKRYVHLDGPVAVYLHRRATDLPPAIG
                     TVVSYEGADSDAARSVAMHIAAAQPRYTTREEVPADVLEAQRREAEATAKEEGKPEQI
                     VPRIVEGKIKKFYADSVLLEQVSVLDESKKTMKDLVDEAGLTVRGFARFEVGHA"
     gene            4261..5031
                     /gene="pyrH"
                     /locus_tag="VFE39_17775"
     CDS             4261..5031
                     /gene="pyrH"
                     /locus_tag="VFE39_17775"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET6260489.1"
                     /translation="MTDLRTGADRARPGFRRVLLKLGGEMFGGGAVGVDPRVVETVAR
                     QIAEVVATGAQVAVVIGGGNFFRGSELQQRGMDRARADYIGMLGTVMNCLALQDFLER
                     EHHIDTRVQTAITMGQVAEAYIPRRAIRHLEKGRVVIFGAGVGMPYFSTDTAGAQRAL
                     EIGAEALLLAKSVDGVFTADPKLDAGAKMFDEITHREVLEQGLKVADATAFSLCMDNK
                     MPIIVFNLLVEGNIARAVRGERIGTLVSTPDYAQEPRE"
     gene            5034..5591
                     /gene="frr"
                     /locus_tag="VFE39_17780"
     CDS             5034..5591
                     /gene="frr"
                     /locus_tag="VFE39_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019818374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET6260490.1"
                     /translation="MIDETLFDAEEKMEKAVSVAKDDLASVRTGRATPNMFSRVAVDY
                     YGSMTPINQLASINVPEARMAVIKPYDTGQLRALEKAIRDSDLGLNPSNDGQIIRVVI
                     PQLSEERRREMVKVARSKGEDARVTIRSIRRKAMDELHRIVRDGDAGEDEVGRAEKEL
                     QAATDRYVHQVDDLVKHKEAELLEV"
     gene            5630..6466
                     /locus_tag="VFE39_17785"
     CDS             5630..6466
                     /locus_tag="VFE39_17785"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET6260491.1"
                     /translation="MVPPARRPSRAGRDLAAAIAVGTALGVVILSSLLIERHFFVGVL
                     AVATAVATWELAGALRRGAGIEVPLGVLLLGGQAMVWLSWPYGLRGLAVSFAVTALAV
                     LLWRMRTGAAHYVRDVTAGLFTAAYVPLFCSFTILMLVADDGLGRVLTFLICGVASDV
                     GGYAAGVLLGRHAMAPTISPKKSWEGFAGSQVAGMLAGALCVHLLLGGVWWAGAITGA
                     LLVVSATLGDLVESMVKRDLGIKDMGTLLPGHGGLLDRMDSLLPTAVVAWVLLSIVVP
                     VS"
     gene            6463..7209
                     /locus_tag="VFE39_17790"
     CDS             6463..7209
                     /locus_tag="VFE39_17790"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET6260492.1"
                     /translation="MIRPFRGVAAAGAAGPIPSPNIWNWPEVYEQENAAQDVDGAVWT
                     VLREVAPWAGADVLDVGCGDGFHLPVFAAAAASVTGVEPHPPLVERARGRVGDRARVL
                     RAGAAALPLPDASVDLVHARTAYFFGPGCEPGLAEAQRVLRPGGVIAIVDLDATVPPY
                     GRWMRADLPRYDPARAESFFERQGFSMRRVPTVWRFPDRASLEAVLQIEFSRAVAERA
                     IAETGGLTLPVGYRVHVRRKQSFHRVTPPL"
     gene            7410..7940
                     /locus_tag="VFE39_17795"
     CDS             7410..7940
                     /locus_tag="VFE39_17795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260493.1"
                     /translation="MRGPVVPYLEPVAGGVWWTVGASALDTGAGTVILAAGLGVTGGL
                     VVALRKRYATGAPLPPGGRGRFLRLLGITVALVAVAGTVLGSVGLGELGVPLACAMIG
                     VALIPLSTQLDERSLLATGGALMVLGAVGALLALNSVGSLYPQGVVGLVAGAIFWAAA
                     AYRTGLLAEARGRVRR"
     gene            8017..8475
                     /locus_tag="VFE39_17800"
     CDS             8017..8475
                     /locus_tag="VFE39_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260494.1"
                     /translation="MVITQERRTSSVLPTLALIWSCLAVSLGVWWWPAQRYPFGPGDP
                     DSFGTVLGILPAALVPPVVVAAGTWGLVAAVLARVRPAGRGAMAVVVGTAVVYAVGFG
                     LVVPGLQPLTLVGYLMAMFGPVALFCAVLAGRGAGAAVRGQWVCWCWWWR"
     gene            8445..9152
                     /locus_tag="VFE39_17805"
     CDS             8445..9152
                     /locus_tag="VFE39_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260495.1"
                     /translation="MGVLVLVVALTWISGLADGAVLRRYLDVITTTLHKAGPSAALLF
                     FLVGGLLWGVLGARTVLRARAGRPTPGWTRPEAAAQWGRVAAVIAAVCALPYGLVRLT
                     WLTPWPLGGSAEALATEPEMRLHGLLLGLAALAGAVLTTGLVSRWGEVWPRWMPVLRG
                     RRVPVAAAVVPGALVAALFTVAAVPMMALAISEGQLWGLLVFPFPVWGPALGAAVLGY
                     AIRRRDEAVPPGTIEVS"
     gene            9149..10255
                     /gene="rlmN"
                     /locus_tag="VFE39_17810"
     CDS             9149..10255
                     /gene="rlmN"
                     /locus_tag="VFE39_17810"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET6260496.1"
                     /translation="MTALPLVFDAPRRATPPRHLADLDPADRRAVVGELGLPAFRADQ
                     LARHYFGRLTADLDAMTDLPAGVRDTLRALLPPLVTPVLEQACDGGATRKTLWKGHDG
                     TRAESVLMGYPDRATVCVSSQAGCGMACPFCATGQGGLQRNLSTAEIVDQVRQAAAAA
                     RDGALGSPARLSNVVFMGMGEPLANYKRVVAAVRRITEPAPDGLGISARGVTVSTVGL
                     VPAIDKLAAEGLPVTLAVSLHTPDDELRDTLVPVNNRWKVSEVLAAARRYVQATGRRV
                     SIEYALIRDINDQPWRADLLGTLLRQQIGAKRVHVNLIPLNPTPGSEWDASPRRVQDE
                     FVARVQAAGISCTVRDTRGREIAAACGQLAASEA"
     gene            complement(10336..11403)
                     /locus_tag="VFE39_17815"
     CDS             complement(10336..11403)
                     /locus_tag="VFE39_17815"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HET6260497.1"
                     /translation="MRLPVMPPVSPMLAKPIGDIPAGQLYEPKWDGFRSIIFRDGDEV
                     EIGSRNERPMTRYFPEVVEAVLAHFPERAVVDGEIVVADTARNTLDFEALQQRIHPAA
                     SRVKLLSEQTPASFIAFDLLALGDDDLTEKPLEDRRAALERAFADAPPPIHLTPVTRD
                     LDTARKWFEQFEGAGLDGLIAKKPDLRYQPDKRVMSKIKHQRTADCVVAGYRVHKSGP
                     DALGSLLLGLFDDRGVLNSVGVVGAFPMATRRSLFAELQPLVTTFDGHPWNWAAHEQG
                     ERTPRKNETSRWNAGKDLSFVPLRPERVVEVRYDYMEGDRFRHTAQFVRWRPDREPES
                     CTYDQLERPVRFNLADVLTSG"
     gene            11805..12362
                     /gene="mug"
                     /locus_tag="VFE39_17820"
     CDS             11805..12362
                     /gene="mug"
                     /locus_tag="VFE39_17820"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020663852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HET6260498.1"
                     /translation="MPRLTRAELEAARDRTIEDVLPGPGDPPLRVLFCGINPGLVSAA
                     TGHHFARPGNRFWPALHGAGFTPRLLLPAEQGELRGLGLGITNMAPRATARADELTVA
                     EMVAGGKRLQELVEQQRPGWAAVVGVTAYRAAFHVPRAVVGPQDERLGPTRIWVLPNP
                     SGLNAHWSLAAMVDEFARLRVAADS"
     gene            complement(12638..13618)
                     /locus_tag="VFE39_17825"
     CDS             complement(12638..13618)
                     /locus_tag="VFE39_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase small subunit domain-containing
                     protein"
                     /protein_id="HET6260499.1"
                     /translation="MTRSPSRSAIELEVGDRVVRLTNPDRVYFPARGETKLDLARYYL
                     AVSDGIVRALRDRPCMLHRFPTGVTGEKVHQKRLPKGAPDWVRTVRVHFPRWNRHADE
                     LCVQHPADVVWAVQMSTVEFHPWNSRCTDTEKPDEWRIDIDPMPEASYTDVRRVAHVA
                     HEVLGELGATGWPKTSGGKGMHIYVRIAPDHGFGDVRRAAHAFAREVGRRCDLVDLTW
                     WRKDRDPASIFVDYNQNARDHTIRSAYSVRGVPEAVVSTPIRWDEVDDADPRDFTIAT
                     VPPRYAELGDLHAGIDDAVFLIDELLEWADRDARDGADEPPTEDVQNLDG"
     gene            complement(13687..13965)
                     /locus_tag="VFE39_17830"
     CDS             complement(13687..13965)
                     /locus_tag="VFE39_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015104418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2631 domain-containing protein"
                     /protein_id="HET6260500.1"
                     /translation="MDLEDGVARNSRELARRPAVDPQDEPSAEWGWHGGFPRGSVIAG
                     WASVVILLLLTIGNHEGNTEDIWLVGFAVLIAFGIVMQTIRRRNAWRR"
     gene            14090..15292
                     /gene="dxr"
                     /locus_tag="VFE39_17835"
     CDS             14090..15292
                     /gene="dxr"
                     /locus_tag="VFE39_17835"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018682007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HET6260501.1"
                     /translation="MSKRSVLVLGSTGSVGTQALDVLAAHPDRFAVTGLAAGGGDLAL
                     LARQVREHGVRRVAVARADAAAALREELRGVEVLAGPDAATELTATAGADTVLNGITG
                     SVGLGPTLAALASGATLALANKESLVAGGALVTGAAAPGQIVPVDSEHSALAQCLRGG
                     RAEEVDRLVLTASGGPFRGRRRSELVGVTVAEALAHPTWDMGPVVTINSANLVNKGLE
                     LIEAHLLFDVPYDRIDVVVHPQSIVHSMVTFVDGSTLAQASPPDMKLPIALALGWPER
                     LPGAVVPCRWDAAQSWTFEPLDDDAFPGVRLARAAGDAGGCLPAVLNAANEEAVAAFV
                     AGSLPFPGICEVLEGVLDAADGWRADPATVAEVLAAEEWARAHAREATARTAGGASIG
                     SGLPGATT"
     gene            15289..16575
                     /locus_tag="VFE39_17840"
     CDS             15289..16575
                     /locus_tag="VFE39_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009943691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET6260502.1"
                     /translation="MMTVIGIVIFFVGILFSIAWHELGHFTTAKWFGIKVPEFMVGFG
                     RTIWSRKVGETEIGLKAVPLGGYIRMIGMIPPAKGQTLGRSRRTGPFQGLIDDARRQS
                     AMDVLPEDADRQFYLRAPWKRIIVMFAGPFMNLILAVVLFAIVLMGIGVPTSTTSVGA
                     VSACVVSATAATEQCPPGAPPTPAAAAGFRPGDRIVAFNGHPYSQFDGRDLQDAIRAS
                     SGPVTITVERNGQRLDLNATPIRTQLASFDGTSQTVQGGFLGVSLVSAYQRQDIGAVF
                     GQIGDIVARTGNAIVQLPSRVPGLFGSVFLGQQRDVNGPIGIVGASRIGGEILASDAP
                     PVAELSVFLQLLAGLNISLFLFNLLPIPPLDGGQIFPAIWESIKRRIARLRGRPDPGP
                     VDVAKLMPVAYVVALVFIGWSALLFVADIINPVQLF"
     gene            16644..17198
                     /locus_tag="VFE39_17845"
     CDS             16644..17198
                     /locus_tag="VFE39_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260503.1"
                     /translation="MSVPAGLFRPDQSPALRAAVPEVYPDARPLAVFDVDGVLADVRH
                     RLHHLEVRPQRWERFFRSADRDPLLTEGAERLRAALVDHDVMYLTGRPERTRRLTEHW
                     LACHDLPVGPLVMRADDDHRPARWLKRDVLRTLATVREVVSVLDDDPAVVSVLEADGW
                     PVELATWLPHSSTLRSAQEDKGRT"
     gene            17295..>18182
                     /gene="ispG"
                     /locus_tag="VFE39_17850"
                     /gene_synonym="gcpE"
     CDS             17295..>18182
                     /gene="ispG"
                     /locus_tag="VFE39_17850"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HET6260504.1"
                     /translation="MSVSLGMPAPPAPTLAPRRNTRQLQVGPVGVGSEHPISVQSMTT
                     TLTSDINATLQQIAELTAAGCDIVRVACPSQDDADALPAIARKSQIPVIADIHFQPKY
                     VFAAIEAGCAAVRVNPGNIRKFDDQVKQIAAAAKDHATPIRIGVNAGSLDRRLMEKYG
                     KATPEALAESAMWEASLFAEHDFHDIKISVKHNDPVVMVRAYELLAQECDYPLHLGVT
                     EAGPAFQGTIKSAVAFGALLRQGIGDTIRVSLSAPPVEEIKVGTQILQSLNLRPRKLE
                     IVSCPSCGRAQVDVYKLADE"
BASE COUNT         2519 a         6372 c         6587 g         2704 t
ORIGIN      
        1 ccggcgctgg tccggagcgg gtctccaccc gcgctgctac tcggggcgcg tgggggccgg
       61 ctggacccgc gggtggcgcg caccgtcgtg caccgcgcgc tcgccgcggt gccgggcgcg
      121 cccgatgtcg ggccgcacgg cctgcggcac gcagcagcca cgcacatgtt ggagggcggc
      181 gcggacctac gttacgtaca ggagttgctc ggtcacgcta agctagcaac cactcagctc
      241 tacacacatg tgacagtcga gcggctgaag gtggttcatg agcaggcgca cccgagggcg
      301 tgacgtcgtc ctggtcgagt cggtgcagct cagggccatg accgggcccg tcgtgacggt
      361 cccggtcgcc ccatctgctg acccggtggt cccggtggtc ccggttgtcg acacggccga
      421 cccggatgcg gtctacgagg tctggaccgc ctatctggac cagccgacac gccaacttcg
      481 cgaccgtctg gtgttgcatt acacgccgct ggtccgttcg gtcgcccatc gcgtcgccgc
      541 cgggcttccg tcctacgtcg agcgcgccga cctcgtccaa tgcggcatct tcgggctcat
      601 cgacgccatc gagcggttcg acccgcagcg ctgcccgcgg ttcgagagct acgccgggca
      661 gcgcatccgc ggcgcgatct tcgacgagct cagggcgcag gactgggtgc cgcgcagtgt
      721 ccgcgggcgc gtgcgggatc ttgaacgtgc gcaggagcgg ttggagagcc ggttgctgcg
      781 cactgcgacc gaccgggagc tggcccagga gctgggcgtg ccggtgcacg agctgcgcat
      841 gctcacgcgg caggtgcagt tgatcagcgt cgaggcgctg gaggagagca gcggcggcgt
      901 gtcggagctg ttcgccgacg acgccgctcc cgacccgatg gtggtcgtgc agtcccacga
      961 gacactgcgg cagctctcgg tggcggtcga gcagctcggc gagcgcgacc ggaccgtggt
     1021 ccggctctac tacctcgaga accggacgct ggccgagatc ggctcgctgc tcggagtgac
     1081 ggagtcgcgg gtatgccagc tccattcacg gctggtggga cggctgcgag gacggctgga
     1141 ggagctcgcc gccggctgag acccaccggc tcccggtcag ctcctcctcg ctgccggtcg
     1201 cgaaccggcc cggtgaacca cgcgccgttc attcagccgt ccggccacgg cacgggcacg
     1261 ggcagcaggc ggacccgcaa cgggcgcagc agcacgagcg ggtcgaggta gtccgcccgg
     1321 tccctgcgga cgccccagtg caggcacgcc ggcgtggcgc agccggcgtg accgggttgg
     1381 agcaggccga tgacctcgcc ggccagaacg atggcgcccg cccggaccgt gggctgcagc
     1441 ggctcgtacg tggtgcgcaa cccgtcgtcg tgctgcaccg acacgaggtc gcggccgccg
     1501 acgggcccgg cgaacacgac cgtgccgccg cgtgcagcga cgatcggttg caggggcgcg
     1561 ccggcgaggt cgacgccgcg gtgtcctggc ccgtagggat gggcgggcgc ccggaacgga
     1621 gtgaccacag ccggagcggg gaccagtggc cacgcgtacc gggccccggg agcggaaaca
     1681 cccgcctctg gcggcggagc gggagccacg agctccggcc gctcctcgtc cgcgggcggt
     1741 ggacccggaa ccgggggagc gagtgcggtg aggtgggccg gtccgcggac cgatgtgaaa
     1801 acgacggcct tgtccgggcc ggtgttgtcc gggccggtgt tgcgcgggcc ggtgttgccc
     1861 cgacgggcct cgcccgaggc cgcctcattc tggctggcac ccttctgacc ggccccgctc
     1921 gagcccgcga ggctggacgg ccccattccg cccagagcgg cggtgactgc gcacaggagt
     1981 cccgccgtca gtgccgcccg cagcgggcgg ccggcccgtg gtcggagcct cagccgcccc
     2041 tgacgtcgct ccagggccat accctcaccc tgcccccgcg gaggcccgcc aacaccggct
     2101 ccgctgcggc tgtggacgcc cgatgacgca tgtggacaac ccggggacgc ggcgagaggt
     2161 gctatgtcac cgcggcgtac acttcccctg caccccgact tgatgtcagg ggtgacttcg
     2221 cgcgctcgcg cgcctgcctg gttcggccat tctctgggcc ggccggcgga acggtggacg
     2281 ggcggtcccg cgctacatca cgggtccggc caccggcgcg acgccagggc ggtaccgccg
     2341 accggcgctc cgcaaaccgc gcgcgtggtc gccgagcgac cacgcccgac cgaaaggtga
     2401 acaccggccg tggccgtcgt caccatgaag cagttgctgg actcgggtgt gcacttcggg
     2461 caccagaccc ggcgctggaa cccgaagatg aagcgctaca tcctcaccga gcgcaacggt
     2521 atctacatca tcgacctcca gcagaccctg tcgtacatcg accgggccta cgagttcgtc
     2581 cgcgagaccg tggcgcacgg cggcacgatc atgttcgtcg gtacgaagaa gcaggcgcag
     2641 gaggccatcg ccgacgaggc gggccgcgtc aacatgccgt acgtcaacca gcgttggctg
     2701 ggcggcatgc tcaccaactt ccagaccgtc cacaagcgtc tccagcggat gaaggagctg
     2761 gagtcgttgg agcagacggg gggcttcgag ggtcgcacca agaaagagat cctcatgctc
     2821 acgcgcgaga agaccaagct cgagaagacc ctcggtggca tccgggacat gaccaaggtc
     2881 ccgagcgcag tctggatcgt cgacaccaag aaggagcaca tcgccgtcgg cgaggcccgc
     2941 aagctgggca tcccggtggt ctccatcctg gacacgaact gcgaccccga cgaggtcgac
     3001 ttcccgatcc ccggcaacga cgacgccatc cgctcggctg cgctgctcac caaggtgatc
     3061 gcgcgggccg cggccgacgg gctcatggcc cgctcggcca ggagtcgtgg cgacggcgat
     3121 gagaagccgg aggccggggt cggcagcgac gagccgctga ccgagtggga gcagaacctg
     3181 ctcaccaacg gcgctggttc cgacggtgcc agcctgacgg ctgccgctcc cgcgaccgag
     3241 gcgcccaacc cggccgccga gcccgcgccg gccagcacca gcaacggcac gcagcagacc
     3301 gcgggctgat cccgctgccg ccgcgggacc gcacccggtc ccgcggcggc gctcgcaccg
     3361 cacctgccgc ccgcccacac ctcagaaacg gacgaagagg caccaccatg gcgaactaca
     3421 ccgccgccga ggtcaagaag ctccgtgagc tcaccggcgc cggaatgatg gactgcaaga
     3481 atgcgctcga gcaggccgac ggcaacatcg agcaggccgt cgaggcgctg cgcgagaaga
     3541 gcgccaagtc cgtcgacaag cgcactgagc gcaccacgac caacggcctg gtcgcggcgt
     3601 ccgggggcac catgatcgag ctcaacagcg agaccgactt cgtggccaag agcgccgact
     3661 tcattcagct ggcggagacc atcctgcagg tcgccgtcga gcagcagccc gccgacgtcg
     3721 aagcgctcaa cgctgccccg cttggcgacg gcacggtcct cgacgccgtc caggcgcttt
     3781 cggggcggat cggcgagaag ctgcagatca agcgttatgt ccacctcgac ggcccggtcg
     3841 cggtctacct gcaccgccgc gccacggacc tcccgcctgc catcggcacc gtggtgtcct
     3901 acgagggagc cgactccgac gccgcccgca gcgtggcgat gcacatcgcc gccgcccagc
     3961 cgcggtacac cacccgcgag gaggtgcccg ccgacgtgct cgaggcacag cgtcgggagg
     4021 ccgaggccac ggccaaggag gagggcaagc ccgagcagat cgtgccccgg atcgtcgagg
     4081 gcaagatcaa gaagttctac gcggactcgg tcctgctcga gcaggtttcc gtgctcgacg
     4141 agtccaagaa gacgatgaag gacctcgtcg acgaggccgg gctcaccgtg cgcgggttcg
     4201 ctcgcttcga ggtcggccac gcctgagcgg ccgggctgcc gagatgagtg acgtgacgtc
     4261 atgacggacc ttcggaccgg cgccgaccgc gcccgccccg gcttccgccg cgtgctgctc
     4321 aagctcggcg gcgagatgtt cggcggtggg gctgtcggcg tcgatccgcg ggtcgtcgaa
     4381 accgttgcgc gccagatcgc cgaggtcgtg gccaccggtg cccaggtcgc ggtggtgatc
     4441 ggcggcggca acttcttccg tggctcggag ctgcagcaac gcggcatgga ccgcgcgcgc
     4501 gccgactaca tcggcatgct cggcaccgtg atgaactgcc tcgcgttgca ggacttcctg
     4561 gagcgcgagc accacatcga cacccgggtg cagacggcca tcacgatggg ccaggtcgcg
     4621 gaggcctaca tcccccgccg cgcaatccgg cacctggaga agggccgcgt ggtgatcttc
     4681 ggcgcgggcg tcgggatgcc ctacttctcc accgacacgg ccggtgccca gcgcgcgctg
     4741 gagatcgggg cggaggcgtt gctgctcgcc aagagtgtcg acggtgtctt caccgccgat
     4801 ccgaagctcg atgccggtgc caagatgttc gacgagatca cccaccgcga ggtgctcgag
     4861 caggggctca aggtcgcgga cgccacggcg ttcagtctct gcatggacaa caagatgccg
     4921 atcatcgtgt ttaacctgct ggtggagggc aacatcgcac gagcagtgcg gggtgagagg
     4981 atcggcactc tagtcagcac gccggactat gcgcaggagc ctagggagta gccgtgatcg
     5041 acgagacgct cttcgacgcc gaggagaaga tggagaaggc cgtctcggtg gccaaggacg
     5101 atctcgcgag cgtccgcacc gggcgggcca cgccgaacat gttctcccgc gtagccgtcg
     5161 actactacgg ctcgatgacg ccgatcaacc agctcgcctc catcaacgtc ccggaggcgc
     5221 gcatggccgt catcaagccc tatgacaccg gccagctcag ggcactggag aaagcgatcc
     5281 gcgactccga cctcgggctc aacccgagca acgacggcca gatcatccgc gtggtgatcc
     5341 cgcagctgtc cgaggagcgg cgccgcgaga tggtcaaggt cgcccgcagc aagggcgagg
     5401 acgcgcgcgt cacaattcgc agcatccgcc ggaaggcgat ggacgagctg caccgcatcg
     5461 tgcgcgacgg cgacgcgggc gaggacgagg tcgggcgggc ggagaaggag ctacaggccg
     5521 ctaccgaccg gtacgtccac caggtcgacg atctggtgaa gcacaaggaa gccgaactcc
     5581 tcgaagtctg acttcggtga cggcctcctc ctccccccac actgcggcca tggtccctcc
     5641 ggcccgccgg ccctcccgcg cgggccgcga cctggctgcg gcgatcgcgg tgggcaccgc
     5701 gctcggcgtc gtgatccttt cgtcgctgct gatcgaacga catttcttcg tcggcgtact
     5761 cgcggtggcc acagccgtcg ccacctggga gctcgcgggt gcgctgcgcc gaggtgccgg
     5821 gatcgaggtg ccgctcgggg tgctgctcct tggcgggcag gcgatggtgt ggctgtcgtg
     5881 gccgtacggc ctgcggggtt tggccgtgtc tttcgcggtc acggcactcg cggtgctgct
     5941 gtggcggatg cgcaccgggg cggcgcacta cgtccgcgat gtcacggccg gtctgttcac
     6001 cgccgcgtac gtgcccctgt tctgctcgtt caccattctc atgctggtcg ccgacgacgg
     6061 cctgggccgc gtgctgacgt tcctgatctg cggggtggcg tccgatgtcg gtggctacgc
     6121 cgccggtgtc ctactggggc ggcacgcaat ggcccccacg atcagcccga agaagtcgtg
     6181 ggagggcttc gccgggtcgc aggttgccgg catgctcgcc ggtgcgctgt gcgtccatct
     6241 tctgctcggc ggagtctggt gggccggtgc gatcaccggt gcgctgctgg tggtcagcgc
     6301 cacgctgggc gacctcgtcg agtcgatggt caagcgcgac ctcggcatca aggacatggg
     6361 cacgctgctg cccggtcacg gcgggctgct ggaccggatg gactcgctgc tgcccaccgc
     6421 ggtcgtggcc tgggtgctgc tcagcatcgt cgtgcccgtg tcgtgatccg gccgttccgc
     6481 ggcgtcgcag ccgccggcgc agccgggccg atccccagcc ccaacatctg gaactggccc
     6541 gaggtctacg agcaggagaa cgccgcacag gacgtcgacg gggcggtgtg gacggtgctg
     6601 cgcgaggtcg cgccgtgggc cggcgcggat gtgctcgacg ttggttgcgg cgacggattc
     6661 cacctaccgg tgttcgcggc ggcggcggcc tctgtgacgg gtgtcgaacc gcacccgccg
     6721 ctggtcgagc gggcccgcgg ccgcgtcggt gaccgcgcgc gggtgctgcg ggcaggcgct
     6781 gccgcgctcc cgctcccgga cgcgagtgtc gacctcgtgc acgcccgcac cgcatacttc
     6841 ttcggccccg gttgcgagcc ggggctggcg gaggcgcagc gggtgctgcg tccgggcggg
     6901 gtgatcgcca tcgtcgacct ggacgccacc gtgccgccct acggccggtg gatgcgtgcc
     6961 gacctcccgc gctacgaccc ggcgcgcgct gagagcttct tcgagcgtca aggcttctcg
     7021 atgcgccggg tgccgaccgt gtggcggttc cccgaccgtg catcgttgga ggcggtgctg
     7081 cagatcgagt tctcccgcgc cgtcgccgag cgggccatcg cggagaccgg cggcctcacc
     7141 ctgcccgtcg gctaccgggt gcatgtgcgg cgcaagcaga gcttccaccg cgtgaccccg
     7201 ccgttgtgac cccgccgtcg tgaccccgcc gtcgtgactc cgccgccatg acccgggcgc
     7261 gcagtggcac ggccggagtt gcaccgcccc agtggcgcca ccggagcgac cgggggcggc
     7321 acggcccgag tggcacatcc gccgtggcgg agctggttca ggtcggtggc ccccgcgagc
     7381 gcctgggccg aactggatac cgtggaccca tgcgtggacc cgtggtgccc tacctcgagc
     7441 cggtcgcagg cggagtgtgg tggacggttg gtgcgtcggc gctcgatacc ggtgctggca
     7501 cggtcatcct ggcggccggg ctcggtgtca ccggcgggct ggtggtcgcc ctgcggaagc
     7561 ggtacgcgac gggagccccg ctgccgccgg gcgggcgcgg gcggttcctg cggctgctcg
     7621 gcatcaccgt ggcgctcgtg gccgtggccg gcacggtgct cggctccgtc ggcctgggcg
     7681 agctcggggt cccgctcgcc tgcgcgatga tcggcgtggc gctcataccc ctgtcgacgc
     7741 agctggacga gcggtcgctg ctggccaccg gcggtgcgtt gatggtgctc ggcgcagtgg
     7801 gcgcgctgct tgcgctgaat tctgtgggca gcctgtaccc acagggcgtg gtcgggctcg
     7861 tggccggtgc aatcttctgg gcagcggccg cgtaccgcac cgggttgctc gcggaggcgc
     7921 gggggcgggt acgccgctga ggctcccacc ggcgggggac cccagtcccc ctcggccggg
     7981 agggcagcgg aaccgggtcg gcggatcgtc tccgccatgg tcatcacgca agaacgcaga
     8041 acgtcgtcag tactgcccac actggccctg atctggtcgt gcctggctgt ctccctcggc
     8101 gtgtggtggt ggccagccca gcgctacccg ttcggtccgg gggatccgga cagcttcggc
     8161 actgtgctgg gcatcctgcc cgctgccctg gtgccccccg ttgtcgtggc cgcggggacg
     8221 tgggggctgg tggccgccgt gctcgcccgg gtgcggccgg cgggacgcgg cgcgatggcc
     8281 gtggtggtcg gcaccgcggt ggtgtatgcg gtgggcttcg ggttggtcgt ccccgggctg
     8341 caaccgctca ccctggtcgg ctacctgatg gcgatgttcg ggccggtcgc gctgttctgc
     8401 gcggtgctgg ccgggcgtgg cgctggcgcg gcggtccgtg ggcagtgggt gtgttggtgc
     8461 tggtggtggc gctgacctgg atcagcgggc tcgccgacgg ggcagtactg cggcgctacc
     8521 tggacgtcat caccacgact ctccacaagg ccgggccgtc cgccgccctg ctgttcttcc
     8581 tcgtcggggg cctcctgtgg ggcgtcctcg gtgcccgaac cgtgctgcgg gcccgcgcag
     8641 gccgtccgac cccggggtgg acgcggcccg aggctgcagc gcagtggggg agggtggccg
     8701 cggtgatcgc ggcggtctgc gcgctgccct acggcctggt ccgcctcacc tggctgacgc
     8761 cgtggccgct cggcggcagc gccgaagccc tggccaccga gccggagatg cggctgcacg
     8821 ggctgctact cggcctcgct gcgctggccg gagcggtgct gaccacgggc ctcgtgtccc
     8881 gctggggcga ggtctggccg cgctggatgc cggtgctccg ggggcgtcgc gtgccggtcg
     8941 cggcggccgt ggtgcccggc gcgctggtgg cggccctgtt cacggtggca gcggtcccga
     9001 tgatggcgct ggcgatcagc gagggtcagc tctgggggct gctcgtcttc ccgttccccg
     9061 tgtggggccc cgcgctcggc gctgcagtgc tggggtacgc catccgccgc cgggacgagg
     9121 ccgttccacc tggcacaatc gaagtgtcat gactgccctg cccctggtgt tcgacgcgcc
     9181 caggcgcgcc acccctccgc gccacctcgc cgatctcgac ccggccgacc gccgggccgt
     9241 ggtcggcgag ctcggcctgc ccgccttccg cgccgaccag ctggcccggc actacttcgg
     9301 acggctcaca gccgacctcg acgcgatgac cgacctgcct gccggtgtcc gcgacaccct
     9361 gcgggcactc ctgccgccac tcgtcacgcc cgtgctggag caggcctgcg acggcggggc
     9421 cacccgcaag acgctgtgga agggccacga cggtacccgg gccgagtcgg tgctgatggg
     9481 ttatccggac cgtgccacgg tgtgcgtgtc gagccaggcg ggctgcggaa tggcctgccc
     9541 gttctgcgcc accgggcagg gcgggctgca gcgcaacctg tccacggccg agatcgtcga
     9601 tcaggtccgg caagccgcgg ccgcggcacg cgacggcgcg ctgggctctc ctgcccggct
     9661 gtcgaacgtg gtcttcatgg gcatgggcga gcccctcgcg aactacaagc gggtcgtggc
     9721 ggccgtccgg cggatcaccg agcccgcccc tgacggcctg ggcatctccg cgcgcggggt
     9781 caccgtgtcc accgtgggtc tggtgccggc gatcgacaag ctggccgccg agggcctgcc
     9841 cgtcacgctc gcggtctcgc tccacacgcc ggacgacgag ctgcgcgaca cgctcgtgcc
     9901 cgtcaacaac cgttggaagg tgagcgaggt gctcgccgcg gcccgccgtt acgtgcaggc
     9961 caccgggcgc agggtatcga tcgagtacgc gctcatccgt gacatcaacg accagccgtg
    10021 gcgggccgac ctgctcggca cgctgttgcg tcagcagatc ggcgccaagc gtgtccacgt
    10081 caacctcatc ccgctcaacc ccacgcccgg cagcgagtgg gacgcctctc cccggcgggt
    10141 gcaggacgag ttcgtggcgc gggtgcaggc ggccgggatc tcctgcaccg tgcgcgacac
    10201 ccgcggccgg gagatcgccg cggcgtgcgg gcagctggcc gcctcggagg cctgacgccc
    10261 cgacctccgg ctgaccgtta tcgggcctgc gcgaaccctt ggcgctcatt cgtcgagggc
    10321 cgctacgccc tgccgtcaac cgctggtgag gacgtcggcg aggttgaagc ggaccgggcg
    10381 ttccagttgg tcgtatgtgc acgactccgg ctcgcggtcc ggccgccagc gcacgaactg
    10441 cgcagtgtgc cggaaccggt cgccctccat gtagtcgtag cggacctcga ccacgcgctc
    10501 cggacgcagg gggacgaacg acaggtcctt gccggcgttc caccggctgg tctcgttctt
    10561 gcggggcgtc cgctcgccct gctcgtgcgc cgcccagttc cacgggtgcc cgtcgaacgt
    10621 cgtcacgagc ggctgcagct cggcgaacag cgaccgccgt gtcgccatcg ggaacgcgcc
    10681 gaccacgccg acggagttga gcacgccgcg gtcgtcgaag aggccgagga gcaacgagcc
    10741 gagcgcgtcc ggcccggact tgtgcacccg gtagccggcc accacgcagt cggcggtgcg
    10801 ctggtgcttg atcttgctca tcacgcgctt gtcgggttgg taacgcaggt ccggcttctt
    10861 cgcgatgagc ccgtccaagc cggcgccctc gaactgctcg aaccacttcc gcgccgtgtc
    10921 gaggtcgcgc gtgaccggcg tgaggtggat gggcggtggc gcgtcggcaa aggcgcgctc
    10981 gagtgctgcg cggcggtcct ccaacggctt ctcggtgaga tcgtcgtcgc cgagggcgag
    11041 cagatcgaag gcgatgaagc tggccggcgt ctgctcggac agcagtttga cgcggctcgc
    11101 ggccggatgg atccgctgct ggagcgcctc gaagtcgagg gtgttgcgcg cggtgtcggc
    11161 gaccacgatc tccccgtcga cgacggcgcg ctcggggaag tgggcgagca ccgcctccac
    11221 gacctccggg aagtagcggg tcatcggccg ttcgttgcgg ctgccgatct ccacctcgtc
    11281 gccatcccgg aagatgatcg accggaatcc gtcccatttg ggctcgtaga gctggcccgc
    11341 ggggatgtcg ccgatcggct tggccagcat cggcgagacg ggtggcatga cgggtaggcg
    11401 cacgcggcca gtctggcgac tgccggcctc gcggtcgagc cgatacacgc atcagcaatg
    11461 ctcggcggcc ctcagagccg gaccgccggc tcggcctcgc cggtgccacg accagcctca
    11521 tcgcattgtc cggttgaacg gcgttcccag agtgaacgcg gcaccagtgt gggcggcgat
    11581 ctccgtgtgg acggcggctc cggttggaac tgagagcccg tccccgttcg gtcgcgaata
    11641 tcgcagggca ttcggttcac ccccggtcgg gacgccgcac tcctgcacgc gatggggagc
    11701 aaccgcgttg aacggttgtg accacccctt cggccgggcg ccggccgtgc ggtggggcag
    11761 acaggggatc atcggggcgt gaccgcttcc gtgaccgcat ctgcatgccc cggctgaccc
    11821 gggccgagct ggaggcggcg cgcgaccgca cgatcgagga cgtgctgccc gggcccggcg
    11881 acccgcccct gcgcgtgctc ttctgcggca tcaacccggg cctcgtgtcg gcggccaccg
    11941 gccaccactt cgcccggccg ggcaaccggt tctggcccgc gctgcacggc gccggcttca
    12001 ccccgcggct gctgctcccc gcggagcagg gagagctgcg cgggctgggg ctgggcatca
    12061 ccaacatggc gccgcgggcc acggcgcgcg ccgacgagct gacggtggcc gagatggttg
    12121 cgggtggcaa gcggctgcag gagctcgtgg aacagcagcg gcccggatgg gcggcggtgg
    12181 tcggcgtgac cgcttaccgg gcagctttcc acgtgccgcg cgccgtggtc ggtccgcagg
    12241 acgagcggct gggccccacg cggatctggg tgctgcccaa tccgagcggc ctcaacgccc
    12301 actggtccct agcggcgatg gtggacgagt tcgcccggct ccgcgtggca gccgactcct
    12361 gacccgacgg gcgacccgcc cacagccccg ggccgggctg gcccacgacg cccaccacgt
    12421 cgctaccggg cacagcgcac cggggttggc ttcggcgggc ggcccggcca cagtccgcgg
    12481 ccaggcggcc gaccacgcac acgactggcc accgcacaca ccgcatgatg tgtgtgcggc
    12541 gaccaagggg tgtgtgcggc ggagcgtgca ccagtggccc gacgcaacac cgcccgacgc
    12601 atgctgcggc ggggggtggc ggttgctgag cgacccatca gccgtccagg ttctggacgt
    12661 cctccgtcgg cggttcatcg gcgccgtcgc gcgcgtcgcg gtcggcccac tcgagcagct
    12721 cgtcgatcag gaagacggca tcgtcgatgc cggcgtgcag gtcgcccagc tcggcgtagc
    12781 ggggcgggac ggtggcgatc gtgaagtcgc gcgggtcggc gtcgtccacc tcgtcccagc
    12841 ggatcggggt ggagacgacc gcctccggca cgccgcgcac cgagtaggcg ctgcggatcg
    12901 tgtggtcgcg cgcgttctgg ttgtagtcga cgaagatcga cgccgggtca cggtccttgc
    12961 gccaccaggt gaggtcgacg aggtcgcacc gcctgcccac ctcacgcgcg aacgcgtgcg
    13021 ccgcccgccg cacatcgccg aaaccgtggt cgggcgcgat ccgcacgtag atgtgcatgc
    13081 ccttgccgcc cgaggtcttc ggccaccccg tcgcgccgag ctcgccgagc acctcgtggg
    13141 cgacgtgcgc cacccggcgg acgtcggtgt agctcgcctc gggcatgggg tcgatgtcga
    13201 tgcgccactc gtcgggcttc tcggtgtcag tgcaccggga gttccacggg tggaactcca
    13261 ccgtggacat ctgcaccgcc cacacgacgt cggccgggtg ctgcacgcac agttcgtcgg
    13321 cgtgccggtt ccaccgggga aaatggacgc ggaccgtccg cacccagtcc ggtgcgccct
    13381 tcggtagccg cttctggtgc accttctcgc ccgtgacgcc ggtggggaag cggtgcagca
    13441 tgcacgggcg atcgcggagg gcgcgcacga taccgtcgga gacggcgagg tagtagcggg
    13501 ccaggtcgag cttggtctcc ccgcgggcgg ggaagtagac ccggtccggg ttggtcagcc
    13561 ggacgacgcg gtcaccgacc tccagctcga tcgcggagcg ggacggtgag cgagtcatcg
    13621 acacaccgcc ctcacgaagc gccgggcggc cgcagcgagg gagtgcgtgg agccggccat
    13681 cgagcctcag cggcgccagg cgttgcggcg gcggatcgtc tgcatcacga ttccgaacgc
    13741 aatgaggacg gcgaagccga ccagccagat gtcctcggtg ttgccctcgt ggttgccgat
    13801 cgtgagcagc agcaggatca cgacgctcgc ccacccggca atcaccgagc cgcgcgggaa
    13861 cccgccgtgc cagccccact ccgccgacgg ctcgtcctgc gggtccaccg cgggtcgcct
    13921 cgccagctct cgggagttcc tcgccacgcc gtcctccagg tccacgatgt gggcctcatc
    13981 ctcgcacagg cgcggagcgg ggatgatggg cggtgatgag tgagcctgcg agcaaatcaa
    14041 tgtcacagcg cctgcgcgaa gcgctgaccg agcgcagcga ggggatgcga tgagcaagcg
    14101 atccgttctc gtgctgggct cgacgggctc cgtcggcacc caggcgctcg acgtcctcgc
    14161 ggcccacccc gaccggttcg cggtgacggg tctcgccgcg ggcggaggcg acctcgccct
    14221 gctcgcccgg caagtgcgtg agcacggtgt gcgccgggtg gcggtggcgc gcgccgacgc
    14281 tgccgccgcg ctccgcgagg agctgcgcgg cgtcgaggta ctggcaggtc cggacgcggc
    14341 caccgagctg acggccaccg cgggcgcgga caccgtgctc aacggaatca ccggatcggt
    14401 cggactcggc ccgacgctcg ccgcgctcgc ctccggggcc acgctcgcgc tggccaacaa
    14461 ggagtcgctg gtggcggggg gagccctggt caccggggca gctgcgccgg gtcagatcgt
    14521 cccggtcgac tccgagcact ccgccctcgc ccagtgcctg cgcggcgggc gggcggagga
    14581 ggtggaccgg ctcgtgctca ccgcgtcggg cggcccgttc cgcgggcggc ggcggtccga
    14641 gctcgtcggc gtcaccgtgg cggaggcgct cgcccatccc acgtgggaca tgggtccggt
    14701 ggtcacgatc aactcggcga acctggtcaa caaggggctc gagctcatcg aggcgcacct
    14761 gctcttcgac gtgccctacg accggattga cgtggtggta cacccgcagt cgatcgtgca
    14821 ctcgatggtc acgttcgtcg acgggtccac gctcgcccag gccagcccgc cggacatgaa
    14881 gctgcccatc gcgttggccc tcggctggcc ggagcggctc cccggagccg tggtgccgtg
    14941 tcgctgggac gccgcccaga gctggacgtt cgagccgctc gacgacgacg ccttcccggg
    15001 cgtgcggctg gcaagggcgg ccggcgacgc gggcgggtgc ctgccggccg tgctcaacgc
    15061 tgccaacgag gaggccgtgg ctgcgttcgt cgccgggtcg ctgcccttcc ccggcatctg
    15121 cgaggtgctc gagggcgtgc tcgacgccgc cgacggctgg cgcgccgatc ccgctaccgt
    15181 tgcagaggtg ctggccgcgg aggagtgggc acgggcccac gcacgcgagg ccaccgcacg
    15241 caccgccggt ggagcgagca tcggtagcgg actacctgga gcaaccacat gatgaccgtg
    15301 atcggtatcg tgatcttctt cgtggggatc ctcttctcga tcgcctggca cgagctcggc
    15361 cacttcacca cagccaaatg gttcgggatc aaggtgcccg agttcatggt cggcttcggg
    15421 cgcacgatct ggtcgcgcaa ggtcggtgag acggagatcg ggctcaaagc ggtgccgctc
    15481 ggcggctaca tccggatgat cgggatgatc ccgcctgcga agggccagac gctcggccgc
    15541 agccgccgga ccggaccgtt ccaggggctc atcgacgacg cgcgcaggca gtcggcaatg
    15601 gacgtgctcc ccgaggacgc cgaccggcag ttctacctgc gggcgccgtg gaagcgcatc
    15661 atcgtgatgt tcgcaggccc gttcatgaac ctcatcctcg cggtggtgct gttcgcgatc
    15721 gtgctcatgg gcatcggcgt acccacgtcg accacatcgg tcggtgcggt gagcgcgtgc
    15781 gtggtctcgg ccacggccgc gacggagcag tgcccgcctg gtgcgcctcc cacgccggcc
    15841 gcggccgcgg ggttccgtcc cggcgaccgg atcgtcgcgt tcaacgggca tccttactcg
    15901 cagttcgacg gtcgtgacct gcaggacgcg atccgggcct cgtcgggccc cgtgaccatc
    15961 accgtcgaac gcaacgggca gcgcctggac ctgaatgcga cgcccatccg tacccagctg
    16021 gccagcttcg acggtacgtc gcagaccgtc cagggcgggt tcctcggcgt cagcctggtg
    16081 tcggcctatc agcggcagga catcggcgcg gtcttcggcc agatcggcga catcgtcgcg
    16141 cggaccggaa acgcgatcgt gcagctgccc tcacgggtgc cggggctgtt cgggtcggtg
    16201 ttcctcggtc agcagcggga cgtcaacggc ccgatcggca tcgtcggtgc ctcccgcatc
    16261 ggcggggaga tcctcgcctc cgatgcccct ccggtcgcgg agctctcggt cttcctgcag
    16321 ctgctggccg ggctgaacat ctcgctgttc ctgttcaacc tgctgccgat ccctccgctg
    16381 gacggcgggc agatctttcc ggcgatctgg gagtccatca agcggcggat cgcgcggcta
    16441 cgtggccgcc ccgaccccgg ccccgtcgac gtcgcgaagc tgatgcccgt cgcctacgtc
    16501 gtggcgctgg tgttcatcgg gtggagcgca ctgctgttcg tcgccgacat catcaacccg
    16561 gtgcagctct tctgatcgtg atgtaaacgt gctcctggcc acggcatggg gtgaggcggc
    16621 cgggggtctg gatactggag ccggtgagcg ttcccgcggg cctcttccgc cctgatcaga
    16681 gtccggcatt gagggcggcc gtgcccgagg tgtacccgga cgcacgtccg ctcgccgtgt
    16741 tcgacgtcga tggcgtcctg gccgacgtcc ggcatcggct gcaccacctg gaggtccggc
    16801 cacaacgctg ggagcggttc ttccggtcgg cggaccgcga cccgctgctg accgagggcg
    16861 ccgagcgact gcgcgcggcg ctcgtcgacc acgacgtgat gtacctgacc ggccgcccgg
    16921 agcgcacccg caggctcacc gagcactggc tggcgtgcca cgacctgccc gtcggtccgc
    16981 tcgtgatgcg ggccgacgac gaccatcggc cggcccgctg gctcaagcgg gacgtgctgc
    17041 ggacgctcgc gaccgtgcgg gaggtggtgt cggtgctgga cgacgacccc gccgtcgtct
    17101 ccgtgctgga ggccgacggg tggccggtcg agctggccac ctggctgccg cactcgtcga
    17161 cattgcgcag cgctcaggag gacaagggcc gcacctagca tcgacggcgg accggcgccg
    17221 cggctgtacc ccacgagagt gcccgcgacc aaggtggagg cggcgccgca cggtgacggg
    17281 ggatactggg ggtcgtgagc gtctccctgg gcatgcccgc acctcccgca ccgaccctgg
    17341 ccccgcgccg gaacactcgg cagctgcagg tcggtcccgt cggcgtcggg tcggagcacc
    17401 cgatctccgt tcagtccatg accaccaccc tgacttcaga catcaacgcc acgttgcagc
    17461 agatcgccga gctcacggcg gcgggctgcg acatcgtccg tgtcgcctgc ccgagccagg
    17521 acgatgccga cgccctgccc gcgatcgcgc ggaagtcgca gatcccggtg atcgccgaca
    17581 tccacttcca gcccaagtac gtgttcgccg cgatcgaggc gggttgtgcg gcggtacggg
    17641 tgaacccggg caacatccgc aagttcgacg accaggtcaa gcagatcgcg gccgccgcaa
    17701 aggaccacgc caccccgatc cggatcggtg tgaacgcggg gtcgctggac aggcgcctga
    17761 tggagaagta cggcaaggcc acacccgagg cgctcgcgga gtcggcgatg tgggaggcga
    17821 gcctgttcgc cgagcatgac tttcatgaca tcaagatctc ggtcaagcac aacgacccgg
    17881 tcgtgatggt gcgcgcctac gagctgctag cgcaggagtg cgactacccg ctgcacctcg
    17941 gtgtgaccga ggcaggcccg gcgttccagg gcacgatcaa gtcggccgtc gcgttcgggg
    18001 cgctgctgcg ccagggcatc ggcgacacga tccgcgtctc actctcggcg ccacccgtcg
    18061 aggagatcaa ggtcggcacg cagatcctgc agtcgttgaa cctgcgtccg cgcaagctgg
    18121 agatcgtgtc ctgcccgtcc tgcggacgcg cgcaggtgga cgtctacaag ctcgccgacg
    18181 ag
//
LOCUS       DASRSC010000367        12531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2450085,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000367 DASRSC010000000
VERSION     DASRSC010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12531
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2450085"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VFE39_17855"
     CDS             <1..659
                     /locus_tag="VFE39_17855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET6260505.1"
                     /translation="SLDRGGIVLVDLGRAVRVTTSGGNGSRRSLESGGERIRRAAYEL
                     FSQYGVRDVGVDAVIARAGTAKMTLYRNFPSKDELILDFLRRRERLWTEEWLQHESRL
                     RGERPQDQLLAIFDLFSEWFERPDFEGCAFLTTMMEINDRDNPVFQASVEHLARIRGY
                     LRGLAEAAGMADPERVARQWHVLMKGSIMAAHEGDAGAASAARELGVLLLRSHGVPVD
                     "
     gene            complement(723..1886)
                     /locus_tag="VFE39_17860"
     CDS             complement(723..1886)
                     /locus_tag="VFE39_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HET6260506.1"
                     /translation="MGFDPRTATGVPTEPVGSIPRPTELQDAYAKYDAGEIGRDDLEK
                     LQDAAVKHSIERFEATGSPIISDGEQRWSSFATYPIADTLAGTGLAPGLGPGGQFFAI
                     FADGHGRQLPKLERGPFRYNQHAADTLRKSIPYASRPMKQAVIAPSMLALLYPLKDPV
                     EGYSREDFEAALIDECETDIREAFAAGAVRVSVDFTEGRLATREDPRNPWTGAGLLPH
                     FIELNNRVMARFSAAERRNIGLHTCPGGDRDSVHSADVPYNNLLRTMFDINAGYFLIQ
                     LASERDRDPVYETIGQHLRDADGVPQMAYIGTTVTQSPRVESPQEIADQLIRAANFIP
                     KEQLGSTDDCGFSPFSIDEKPNHGSPDYARDVAFQKITSRVEGSRLAAEKLGL"
     gene            2317..2760
                     /locus_tag="VFE39_17865"
     CDS             2317..2760
                     /locus_tag="VFE39_17865"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET6260507.1"
                     /translation="MAELEPRNFLQPCLLLLLREQPDHGYELAARLRPMHDGDGDAGG
                     VYRALRSLERQGLVRSEWHTSDVGPARRTYYVTPDGVTSLDSLADSLGQTHEALHVFL
                     DRYARVVAAARRGCPEGSRIDRRADSCGRGNGVHAARRADRPADR"
     gene            2681..3247
                     /locus_tag="VFE39_17870"
     CDS             2681..3247
                     /locus_tag="VFE39_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET6260508.1"
                     /translation="MTVELTAADAETAFTRRDGPIGLLTADAPALAAACHAMARRFHR
                     GGRLIAFGTGCSAADAAHVVVEFVHPVIIGKRALPALSLPGGSAGFTAELGLFGEAED
                     IALAIAPDGGDEAVADALAAARERGLLTVALLGGAVDGPVALLPDLDHVIAVHSDDPR
                     IVREAQVTSYHVLWELVHVFLDAPEVLA"
     gene            3244..3528
                     /locus_tag="VFE39_17875"
     CDS             3244..3528
                     /locus_tag="VFE39_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004559482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HET6260509.1"
                     /translation="MNTASSTGPSALSPDPHPGEVCITCSDMAVAVTVTALLGDGLAL
                     VDTGAGPGTEEEVSVALVDAGVGHTVLVHAGEAIARVEPPGPIQHRSRSS"
     gene            3525..4178
                     /locus_tag="VFE39_17880"
     CDS             3525..4178
                     /locus_tag="VFE39_17880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET6260510.1"
                     /translation="MSVESLYPFLHSGATDLNVLLADVARSTAEKVGEIGRLRHEVLA
                     AHAAELAACATAMAERFDRGGRLLAFGNGGSSTDAAAMVALCVDPGPAWRPLPALALA
                     ADVATLTALANDVGFENVFARPVAAAGRPADIALGLSTSGGSANVLRGLAVAHERGLL
                     TVGLAGYSGGAMAEAAARGEIDHLFVMPSASVHRIQEAQTTTYHVLWELVQRALGHR"
     gene            4175..6481
                     /gene="hypF"
                     /locus_tag="VFE39_17885"
     CDS             4175..6481
                     /gene="hypF"
                     /locus_tag="VFE39_17885"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HET6260511.1"
                     /translation="MTEPSLQVQPHTEGRVRARIRVSGIVQGVGFRPFVHGLAGQLGL
                     VGFVGNDESGVVIEAEGTAPALDAMLAALRDRPPALALVTHIAATNMPLRGDTAFAIA
                     ASSARGPRATLISPDTATCAECLAELRDPADRRYRHPFITCTNCGPRFTIVTGVPYDR
                     VATTMAGFPMCPACAAEYHDPADRRFHAQPVCCPDCGPTLRLVDADGHDLPGDPITGA
                     ARMLIDGGVLAVKGLGGYHLAALAGNEQAVAELRARKHREDKPFAVLAPGLAAAATLA
                     ELGPAEAAELASRRAPIVLLRRRRSAPLAAAVAPGNDHIGLMLPYTPVHHLLLDDLAA
                     PIVLTSGNLSDEPIAHLDDDAAARLGGIADAFLTHDRPIRTRADDSVLRVVAGRTYPI
                     RRSRGYTPEPLQIGVAAGEPVLGCGAELKNTFCVLRGAEAFPSHHVGDLENAETLRAY
                     TDGIDHLGRLLDVTPTVLAHDLHPEYLSTKYAIERGAVEPGLRLVGVQHHHAHLAACL
                     AENGEAGPAIGIACDGLGYGTDGVLWGGEVLEVSLTGFRRLAHLEPAPLPGGATAIRQ
                     PWRMAAAWLHATGTDPAGLAVAARNSPVWTQVERLLSSDLEVPTTTSAGRLFDAVAAL
                     VGIRDRVNYEGQAAIELEQAASAVGECEGYPVRLDDQLIRAGDLIRAVVADLRVGTPV
                     PVIAARFHTGLHDALVAAAVRSAADTGLEVVALSGGVFQNLRLLHGLSARLTAVGLRV
                     LVHSRVPCNDGGISLGQVAVAAASTAGT"
     gene            complement(6544..6957)
                     /locus_tag="VFE39_17890"
     CDS             complement(6544..6957)
                     /locus_tag="VFE39_17890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010241882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HET6260512.1"
                     /translation="MTTGEGARQVLLDTSVVITPPTAGLASIADYVAVSAITVAELEY
                     GVGAAGDAEERLRRRRRLGVIVDAMEVIPLDIAVAASYGLVANMVRSTGRNPRPRRLD
                     LLIAATAERHGLSLATRNADDFRVLDRVVHVIEVQ"
     gene            complement(6954..7253)
                     /locus_tag="VFE39_17895"
     CDS             complement(6954..7253)
                     /locus_tag="VFE39_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HET6260513.1"
                     /translation="MAEPIRQSELRNNNAEIMRRVAEGESFTVTVHGRAVADLVPHQR
                     TSPRRRLIPAAALDEILAASGPAPDPGRWARDMADADELFGDDRPSDPWDRAGER"
     gene            complement(7312..9135)
                     /locus_tag="VFE39_17900"
     CDS             complement(7312..9135)
                     /locus_tag="VFE39_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007023881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HET6260514.1"
                     /translation="MSDVPLDHHALLSDCGAAALVTSAGSVDWLCLPRFDSPPVLARL
                     LDQAAGHFLIAPAIGGSASRRRYRPSGLVLETTWDAPDGDLLLTEAMALGREDRGHQL
                     GRNAPGVLLRAVRCVRGEVTVRVEYAPRPEFGLVQPWLSRERHAVVAHGGATVLILST
                     ELDMDVDEGTAHAVVTLTEGQELAFALTQGSAWDPAPAPWSSRQIRQGLDTTDEAWRS
                     WSQLHQRYEGPHRDLVHHSGVVLQGLTCARTGAVAAAPTTSLPEGVGSGRTWDYRFTW
                     VRDASMTLQGLWIAACPDEAGRFFSFLATAAATQLHLGQDLQIMFGVGGERDLFEREL
                     PHLSGWRGSGPVRTGNDAWRQRQLDVYGALLDAAYTLRGQLGELDDPTRTFLVAAVDA
                     AAARWKDDDQGIWEMRGPGRPFLHSKLMCWVAVDRGLAMSDVLQPDQDRAATWAAAHR
                     EIRDAILTQGWSDHAGAFTQAFGSDELDASVLLLAIVGFLPPDHPGLHATIDAVERGL
                     ADERGLLYRYRVDDGFDSTEGTFLLCTFWLAHALAITGQTARARVVLDRAARCATPLG
                     LFAEQIDSTTGELLGNFPQAFSHLGLVTAAHALAEAERAAR"
     gene            complement(9216..9977)
                     /gene="hypB"
                     /locus_tag="VFE39_17905"
     CDS             complement(9216..9977)
                     /gene="hypB"
                     /locus_tag="VFE39_17905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007034619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HET6260515.1"
                     /translation="MTDLSAGQGHRHGHGHDDHSHGHGHTAPVRTEPDTRTVALEVDL
                     LAKNDDLAGRNRRWLADRGVIALNLMSSPGSGKTTLLTRTIRDLGDELRCAVLEGDQE
                     TLLDAERIGATGARVVQINTGAGCHLDADMVAAGLHALAPEPDSLLFVENVGNLVCPA
                     LFDLGEAGRVVIMSVTEGADKPLKYPHMFRTADLMLVNKVDLLPYVDFDVADCVSYAR
                     RANPAVEVLEISATRGDGLDAWYDWLRHRRGRLRD"
     gene            complement(10022..10354)
                     /locus_tag="VFE39_17910"
     CDS             complement(10022..10354)
                     /locus_tag="VFE39_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HET6260516.1"
                     /translation="MHELSITQTVVTTVTQRMRDARVRRIRLEVGRLSGLVPDAVRFC
                     FEMVAAGTTCEGAALEIDEPPGRAVCRTCGAAFETGEVLPLCDDCGSADVEVQGGREL
                     RIREVEVV"
     gene            complement(10368..11540)
                     /gene="hypE"
                     /locus_tag="VFE39_17915"
     CDS             complement(10368..11540)
                     /gene="hypE"
                     /locus_tag="VFE39_17915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HET6260517.1"
                     /translation="MTQDLGSNIPVRANWAEEAVAAHAGAEHLTREEQVLARIAKARA
                     RKAKIKEERITLSHGAGGKATQTLIEAVFLDAFRNPVLEQLEDGAQLAPTWSNGVTGR
                     LAFTTDSYVVSPLFFPGGDIGDLAVNGTVNDLAMCGARPLYLSCGFILEEGFPVADLQ
                     RIVASMAAAAEAAGVQIVTGDTKVVERGKADGCYINTAGVGVLERPVVLSAAAAQPGD
                     VVLVSGPIGDHGVTVMLARGELDISADIVSDTAPLHEITAGLLDAAGDGVRLLRDATR
                     GGVATICNEVAVASQVAVVLDEAALPVRPVVNGASELLGIDPLYVACEGRLVAVVAAE
                     QADAALAALHAHPLGDGAAVIGTIKDDPPGLVLLRTAFGGTRIVDLLVGDPLPRIC"
     gene            complement(11537..>12531)
                     /gene="hypD"
                     /locus_tag="VFE39_17920"
     CDS             complement(11537..>12531)
                     /gene="hypD"
                     /locus_tag="VFE39_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009945356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HET6260518.1"
                     /translation="HGLEDHLPESVRLVHGPGCPVCVLPMGRVDDAIAIAKDPDVIMT
                     AFGDMMRVPGGAGSFFDAKAEGADIRMVYSPLDALKIARKNPDKRVVFMAIGFETTAP
                     STAMTVLRAAAEGLDNFSIFCNHVTIIPAIKAILDSPDLRLDGFIGPGHVSTVIGCRP
                     YEWIARDAGKPLVCAGFEPLDILQSVYQLLLQLKEGRCEIENQYTRVVPWNGNAKALQ
                     VIGEVMELRPHFEWRGLGFISHSALQVRERYAQFDAERIFSVPGVRVADPKACQCGEV
                     LKGVLKPWECKVFGTACTPETPIGTCMVSSEGACAAYYNFGRFSRERVKEATRT"
BASE COUNT         1757 a         4486 c         4414 g         1874 t
ORIGIN      
        1 cgtcgcttga tcgtggcggt atcgttcttg tggacctcgg cagggcggtg cgggtgacga
       61 catcaggcgg caacggctcc aggcgatcgc tggagagcgg cggcgagcgc atccgccgtg
      121 cggcctacga gctgttcagc caatacgggg tacgggacgt cggggtcgat gccgtgatcg
      181 cccgcgccgg caccgccaaa atgacgttgt accgcaactt cccgtcgaag gacgagctga
      241 tcctcgattt cctccgccgc cgcgaacggc tgtggaccga agaatggctc cagcacgaga
      301 gccggttgcg tggcgaacgt ccccaggatc agctgctggc catcttcgat ctgttctccg
      361 aatggttcga gcgccctgat ttcgagggtt gtgcgttcct caccacgatg atggagatca
      421 acgatcggga caacccggtg ttccaggcga gcgtggagca cctcgcgcgc atccgcgggt
      481 acctccgtgg gctcgccgag gcggccggca tggctgaccc ggagcgcgtc gcgcggcagt
      541 ggcacgtgtt gatgaagggc tccatcatgg cggcccacga gggcgacgcc ggtgcggcgt
      601 cggccgcgcg tgagctcggt gtgctgctgc tgcggtcgca cggcgtcccc gtcgactgag
      661 ccgctgaaaa ggccagggcc ggcccgaccg tcgattcggt cgaagcggcc ctggggtatc
      721 ggttacaggc cgagcttctc cgcggcgagg cggctgccct cgacccgcga cgtgatcttc
      781 tggaaggcca cgtcgcgggc gtagtcaggc gaaccgtggt tgggcttctc gtcgatcgag
      841 aacggcgaga agccgcagtc gtcggtggat ccgagctgtt ccttgggaat gaagttcgcc
      901 gcgcggatga gctgatcggc gatctcctgc ggagactcca cccggggact ctgcgtgacg
      961 gtggtgccga tgtaggccat ctgcggcacg ccgtcggcgt cgcgcagatg ctggccgatg
     1021 gtctcgtaca ccgggtccct gtcccgctcg gacgcgagct ggatcaggaa gtaccccgca
     1081 ttgatgtcga acatcgtgcg gagcaggttg ttgtagggga catcggcgga gtgcaccgag
     1141 tcgcggtcgc caccggggca ggtgtggagg ccgatattgc ggcgttccgc ggcggagaac
     1201 cgggccatca cgcggttgtt cagctcgatg aaatgcggca acagacccgc cccggtccac
     1261 gggttgcgcg ggtcctcgcg cgtggcgagc ctgccctcgg tgaagtccac cgacacccgc
     1321 acggccccgg cggcgaacgc ctcccggatg tcggtctcgc actcgtcgat cagcgcggcc
     1381 tcgaaatcct cgcggctgta gccctccacc gggtccttca gcggatacag cagcgccagc
     1441 atcgacggcg cgataacggc ctgcttcatc ggcctgctcg catacgggat cgacttgcgg
     1501 agggtgtccg cggcgtgctg gttgtaccgg aacggacccc gctcgagctt cggcagctgc
     1561 ctcccgtggc cgtccgcgaa gatcgcgaag aactggccgc ccgggccgag ccccggcgcc
     1621 aggccggtgc cggccagggt gtccgcgatc gggtacgtcg cgaacgacga ccaccgctgc
     1681 tcgccatcgg agatgatggg tgagccggtg gcctcgaacc gctcgatcga gtgcttcacg
     1741 gctgcgtcct gcagcttctc gaggtcgtcc cggccgatct cacccgcgtc gtatttcgcg
     1801 taggcgtcct gcagctcagt gggccgggga attgatccca ccggctcggt ggggactccc
     1861 gtggccgtgc gcgggtcgaa gcccatggat ggatctcctc cttgagattc cttggcacgc
     1921 gtacgccgtg gaccggcgtg atccatatcc tcggtctgct tgatggcgaa tgctcgatca
     1981 cgcacaccgg acgcagctcg tccaccgcgc gcaattggcc acgcgcagtg gagggagaca
     2041 ggtctgtctc agacctgttc ggcaaagagt agtcgcctct cgatcggcgg tcaacgagcg
     2101 acttatccgc gggaacggtg acctaccgcg accgtgattc tcccgttctt gggcggattc
     2161 cagcgggtgt gatagcgact acacctgacg gggtcgcccc gaacaggtgg atcccgacca
     2221 ccggggagac cgacctgtct tgtggttgcc gtcacacagt ggcaacatcg gcaaagtcag
     2281 gcgcccacca ccgccgaccg gagggcatcg ccccacatgg ccgagctcga accgcgcaac
     2341 ttcctccagc cttgtctgct gctgctcctc cgagagcagc ccgaccacgg ttacgagctc
     2401 gctgcgcggt tgcggcccat gcacgacggc gacggcgacg ccggcggcgt ctaccgcgcg
     2461 ttgcgcagcc ttgaacggca gggcctggtc cgctcggaat ggcacacgtc cgacgtcggg
     2521 ccggcgcggc gcacctacta cgtcacgccc gacggcgtga cctccctcga ctcgctcgcc
     2581 gacagcctcg ggcagacgca cgaggctctg cacgtcttcc tcgatcgcta cgcgcgggtg
     2641 gtcgccgccg ccaggcgggg atgccccgag gggagccgaa ttgaccgtcg agctgacagc
     2701 tgcggacgcg gaaacggcgt tcacgcggcg cgacgggccg atcggcctgc tgaccgctga
     2761 cgcccccgct ctcgccgccg cctgccacgc catggcgcgg cggtttcatc gcggcggcag
     2821 actgatcgcg ttcggcaccg gctgttccgc cgctgacgcc gcgcatgtcg tcgtggagtt
     2881 cgtgcatccg gtgatcatcg gcaagcgggc gctgccggcg ctgtcgttgc cgggtgggtc
     2941 cgccgggttc accgccgagc tcggtctgtt cggcgaggcc gaggacatcg ctctggcgat
     3001 cgcgcccgac ggcggcgacg aagcagtcgc cgacgcgctg gcggccgccc gggagcgcgg
     3061 gctgctgact gtggcgctgc tcggcggagc ggtcgatggc ccggtcgccc tgttgcccga
     3121 tctggaccac gtcatcgcgg tgcactcgga cgacccccgc atcgtgcgcg aggcccaggt
     3181 cacctcgtac cacgtgctct gggagctcgt acacgtcttc ctcgatgccc cggaggtact
     3241 ggcatgaaca cggcttcgag caccggcccg agcgccctgt cgcccgaccc gcaccccggc
     3301 gaggtctgta tcacctgctc cgacatggcc gtcgcggtta cggtcaccgc tctgctcggc
     3361 gacggtctcg ccttggtcga caccggcgcg gggccgggca ccgaggagga ggtcagtgtc
     3421 gcgctcgtcg acgcaggggt tggtcacacc gtcctcgtcc atgcgggcga ggccatcgcc
     3481 agggtcgaac cgcccgggcc gatccagcac cggagccggt cgtcatgagc gtcgagtccc
     3541 tctacccctt cctgcactcg ggggccaccg acctgaacgt gctgctggcc gacgtcgccc
     3601 ggtcgacagc ggagaaggtc ggagagatcg ggcggctgcg ccacgaggtg ctcgccgccc
     3661 atgccgctga gctggcggcc tgcgccacgg cgatggcgga gcggttcgac cgcggcgggc
     3721 ggttgctggc cttcggcaac ggcggctcga gcacggacgc cgccgcgatg gtcgcgctct
     3781 gcgtcgaccc cgggccggcc tggcggccgt tgcccgccct cgcgctcgcg gccgacgtcg
     3841 ccacgctcac cgcgctcgcc aacgacgtcg gtttcgagaa cgtcttcgcc cggccggtgg
     3901 cagcagcggg ccggccggcc gatatcgcgc tgggcctgtc gacgagcggg gggtcggcca
     3961 acgtgctgcg cgggctggcc gtcgcccacg agcgtggcct gctcaccgtc ggactggccg
     4021 ggtactccgg cggcgcgatg gcagaggcgg ccgcccgcgg cgagatcgac cacctcttcg
     4081 tgatgccgtc ggcgtcggtg caccgcatcc aggaggcgca gaccaccacg taccacgtgc
     4141 tgtgggagct ggtgcagcgc gcgctgggcc atcggtgacc gaaccgtcgt tgcaggtcca
     4201 gccgcatacc gaggggcggg tacgggcccg gatccgggtc agcgggatcg tgcagggcgt
     4261 cgggttccgg ccgttcgtgc acgggctggc cgggcaactg gggctcgtcg gcttcgtggg
     4321 caacgacgag tccggtgtgg tcatcgaggc cgagggcacg gcgcccgccc tggacgccat
     4381 gctcgccgcg ctgcgcgacc ggccgcccgc gctggcgctc gtcacccaca tcgcggcgac
     4441 gaacatgccg ctgcgcggcg acaccgcctt cgccatcgcg gccagctcgg cccggggccc
     4501 ccgggccacc ctcatctcgc ccgacacggc cacctgcgct gagtgtctgg ccgagctgcg
     4561 ggacccggcc gaccgccgct atcgccaccc gttcatcacc tgcacgaact gcgggccccg
     4621 gttcacgatc gtcaccggcg tgccctacga ccgggtcgcc acaaccatgg ccggcttccc
     4681 gatgtgcccc gcctgcgctg cggagtacca cgacccggcc gaccggcggt tccacgcgca
     4741 gccggtgtgc tgtccggact gcggtcccac gctgcggttg gtcgacgccg acggccacga
     4801 cctccccggg gacccgatca ccggcgcggc gcggatgctc atcgacggcg gggtgctggc
     4861 cgtgaagggg ctaggcggct accacctggc ggcgctcgcc ggcaacgagc aggccgtcgc
     4921 ggagctgcgg gcccgcaagc accgggagga caagccgttc gcggtgctgg ctcccggcct
     4981 cgcggcggcc gccacattgg cggagctcgg gccggccgag gctgccgagc tcgcgtcacg
     5041 gcgcgcgccg atcgtgctgc tgcgcaggcg ccgtagtgcg ccacttgccg ccgccgtcgc
     5101 ccccggcaac gaccacatcg ggctcatgct gccctacacg cccgtgcacc acctgctgct
     5161 cgacgacctc gccgctccga tcgtgctgac cagcggcaac ctctcggacg aaccgatcgc
     5221 ccacctcgat gacgatgccg ctgcccggct gggcggcatc gcggacgcct tcctgaccca
     5281 cgaccggccg atccgcacgc gcgccgacga ctcggtgctg cgggtggtgg ccgggcggac
     5341 ctacccgatc cgccgctccc gcggctacac cccggagccc ctgcagatcg gcgtcgccgc
     5401 cggggagccg gtgctcggct gcggcgcaga gctgaagaac acgttctgcg tgctgagggg
     5461 cgcggaggcc ttcccctcgc accacgtcgg tgacctggag aacgccgaga cgctgcgggc
     5521 gtacaccgac gggatcgacc acctcggacg cctgctcgac gtgacgccca ccgtgcttgc
     5581 ccacgacctg cacccggagt acctgtccac caagtacgcg atcgagcgcg gcgcggtcga
     5641 gcccggtctg cgcctggtcg gcgtgcaaca ccaccacgcg cacctggcgg cgtgcctggc
     5701 ggagaacggc gaggcagggc ccgcgatcgg gatcgcctgc gacggcttgg gctacggcac
     5761 cgacggtgtg ctgtggggtg gtgaggtgct ggaggtctcc ctcaccggct tccggcggct
     5821 cgcgcacctc gagcccgcgc cgctgcccgg tggggccaca gccatccggc aaccgtggcg
     5881 gatggctgcg gcctggctgc acgccactgg caccgacccg gccggccttg ccgtcgcggc
     5941 gcggaactct ccggtgtgga cgcaggtgga gcggctcctg tcatcggatc tggaggtgcc
     6001 caccacgacg agcgcaggtc gcctcttcga cgccgtcgcg gccctcgtcg ggatccggga
     6061 ccgggtgaac tacgagggcc aggcggccat cgagctggag caggcggcgt cggcagtcgg
     6121 ggagtgcgag ggctaccccg tccggctcga cgaccagctg atccgcgcag gcgacctgat
     6181 acgcgccgtc gtcgccgacc tgcgggtcgg tacccccgtt ccggtgatcg ccgcccgctt
     6241 ccacaccggg ctgcacgacg ccctggtggc cgcggctgtg cggtcggcgg ccgacaccgg
     6301 gctcgaggtg gtggccctgt ccggcggggt gttccagaac ctccggctcc tccacggcct
     6361 gtccgctcgg ctcaccgcgg tggggctgcg ggtgctcgtg cactcccggg tgccgtgcaa
     6421 cgacggcggg atcagcctcg gccaggtggc ggtggccgcc gcgagcacag ccggaacctg
     6481 acgaccggac gaacgcgcac tggttcacgt cggcagtaga acccgtgcgc actcgccatg
     6541 gcgtcactgc acctcgatca cgtggacgac gcggtccagc acgcggaagt cgtcggcgtt
     6601 gcgcgtggcg aggctcaggc cgtggcgctc ggcggtggcg gcgatgagca ggtcgaggcg
     6661 ccgcggacgg gggtttcggc ccgtagatcg aaccatgttg gcgactaggc cgtaggacgc
     6721 ggcaacagcg atgtcgagcg ggatcacctc catggcgtcg acgatgacgc ccaggcggcg
     6781 cctgcgtcgt agccgctcct cggcatcccc ggcggcgccc accccgtact ccagttcggc
     6841 gactgtgatc gcgctgaccg ccacgtagtc ggcgatggac gcaaggcccg cggtgggcgg
     6901 cgtgatgaca acggacgtgt ccagcaggac ctgacgggcg ccctcgcctg tcgtcaccgt
     6961 tcgccggccc gatcccacgg gtcggaggga cggtcgtcgc cgaagagctc gtcggcgtca
     7021 gccatgtcgc gcgcccaccg gccggggtca ggcgcgggcc cggatgctgc caggatttcg
     7081 tcgagggcgg cggccggtat caggcgcctc ctggggctcg tacgttgatg cggcaccaga
     7141 tcggcgactg cgcgtccgtg cacggtcacg gtgaaggact ctccttcggc cacccggcgc
     7201 atgatctcgg cgttgttgtt gcgcagctcg gactgcctga tgggctcggc catgttcgcc
     7261 acaatagcta tgagatagcg gtaacgctat ctcggcacac tgtgctcggc ctcaacgagc
     7321 ggcgcgttcc gcctcggcga gagcgtgggc cgcggtgacg aggccgagat ggctgaaggc
     7381 ctggggaaag ttgcccagga gctcgcccgt cgtgctgtcg atctgctcgg cgaacagtcc
     7441 gaggggggtc gcgcagcgtg ctgcgcggtc cagtacgaca cgggcgcgag cggtctggcc
     7501 ggtgatcgcg agggcgtgag ccagccagaa ggtgcagagc aggaaggtgc cctccgtgga
     7561 gtcgaaaccg tcgtcgacgc ggtagcggta gagcagcccg cgctcgtcgg ccaggccgcg
     7621 ctcgacggcg tcgatggtcg cgtgcagccc gggatgatcg gggggcagga acccgacgat
     7681 ggcgagcaac agcaccgagg cgtccagctc gtctgagccg aaggcctggg tgaacgcccc
     7741 cgcgtggtcg ctccagccct gcgtgaggat ggcgtcgcgg atctcgcggt gcgccgccgc
     7801 ccaggtggcc gcccggtcct ggtccggttg cagcacatcg ctcatcgcca gcccccggtc
     7861 cacggccacc cagcacatca gcttgctgtg caggaagggg cggcccggac cgcgcatctc
     7921 ccagatcccc tggtcgtcgt ccttccaccg ggcggcagcg gcatcgacgg ccgcgacgag
     7981 gaacgtgcgt gtcgggtcat ccagctcacc caactggccc cggagcgtgt aggcggcgtc
     8041 gaggagcgcg ccgtagacgt cgagctggcg ttgtcgccag gcatcgttgc cggtccggac
     8101 cgggccactg ccccgccagc ccgacaggtg ggggagttcc cgttcaaaca ggtcccgctc
     8161 gccgccgacg ccgaacatga tctgcaggtc ctgcccgagg tggagttgcg tcgccgccgc
     8221 ggtcgcgagg aaggagaaga accggcctgc ctcgtccggg cacgccgcga tccacagccc
     8281 ctgcagggtc atgctggcgt cccgtaccca ggtgaaccgg tagtcccagg tacggccgct
     8341 ccccacgccc tcgggcaggc tggtggtcgg ggctgcggcc accgcgccgg tgcgtgcgca
     8401 ggtgaggccc tgcagcacca ccccgctgtg gtgtaccagg tcgcggtgcg gaccctcata
     8461 gcgttggtgc agctgcgacc agctccgcca tgcctcgtcc gtggtgtcga gaccctgtcg
     8521 gatctgccgg gagctccatg gggccggggc cgggtcccag gcgctcccct gggtcagggc
     8581 gaaggccagc tcctgccctt ccgtcaacgt caccaccgcg tgggcggtgc cctcgtccac
     8641 gtccatgtcc agttccgtgc tgaggatcag caccgtcgca cccccgtgcg ccaccacggc
     8701 atgacgctcg cggctcagcc agggctgcac caacccgaac tccgggcgcg gggcgtactc
     8761 gacgcgcacg gtcacctctc cgcggacgca gcgcacggcc cgcagcagca ctccaggcgc
     8821 gttccggccg agctggtgac cccgatcctc ccggcccagc gccatggcct cggtcagcag
     8881 caggtcaccg tccggggcgt cccatgtcgt ctcgaggacg agcccggagg gccggtagcg
     8941 acgcctgctg gccgatccgc cgatggccgg ggcgatcagg aagtgtcctg ccgcctggtc
     9001 gagcagtcgc gccaggaccg gcgggctgtc gaaccggggc aggcacagcc agtccacgga
     9061 ccctgcgctg gtcaccagcg ccgccgcccc gcagtccgac aacagcgcgt ggtggtccag
     9121 cggtacgtcg ctcatcgcac tccctcgctg cgctcggtcg acgcttcgcg aaggcgctgt
     9181 gacactgatt cgctcgcacg ctcgctcacc gcggctcagt cccgcagcct gccccgacgg
     9241 tgacgcagcc agtcgtacca cgcgtcgagc ccgtccccgc gggtcgcgga gatctccagc
     9301 acctcgacgg cggggttggc gcggcgggcg taggacacgc agtcggccac gtcgaagtcg
     9361 acgtagggga gcaggtcgac cttgttcacc agcatcaggt cggccgtgcg gaacatgtgc
     9421 gggtacttca gcggcttgtc ggccccctcg gtcaccgaca tgatcaccac gcggccggcc
     9481 tccccgagat cgaacagggc cgggcagacc aggtttccga cgttctccac gaacagcagg
     9541 gagtccggct cgggggcgag ggcgtggagc cccgcggcga ccatgtcggc atcgagatgg
     9601 cagcccgcgc cggtgttgat ctggacgacc cgcgctccgg tcgcgccgat ccgctcggcg
     9661 tcgagcaggg tctcctggtc accctccagg accgcgcacc gcagctcatc gccgagatcg
     9721 cggatcgtac gggtgagcag ggtggtcttg cccgagccgg gcgagctcat caggttgagc
     9781 gcgatgacgc cccggtccgc gagccaccga cggttgcgcc cggcgaggtc gtcgttcttc
     9841 gccagcaggt cgacctcgag cgcgacggtc cgcgtgtccg gttccgtgcg gacgggagcc
     9901 gtgtgcccgt gcccgtggct gtggtcgtcg tgcccgtgcc cgtgcctgtg cccttgcccc
     9961 gccgacaggt ccgtcatccg cacgccggcg tcatcggaac agccgcatgt cgcacacacg
    10021 ctcagaccac ctcgacctct cggatccgca gctcccgccc gccctgcacc tcgacgtcgg
    10081 cgctgccgca gtcgtcgcac aggggcagca cctcgccggt ctcgaacgcc gccccacagg
    10141 tgcggcacac cgcccgcccc ggcggctcgt cgatctcgag ggccgcgccc tcgcatgtgg
    10201 tgccggccgc gaccatctcg aagcagaacc ggacggcgtc cggcacgaga ccggacagcc
    10261 ggccgacctc cagccggatc cggcgcacac gtgcgtcccg catccgctgg gtcacggtgg
    10321 tgacgacggt ctgggtgatc gacagctcgt gcatcgccgg gcgagggcta acagatccgg
    10381 ggcaggggat cgcccaccag caggtccacg atccgcgtgc cgccgaacgc cgtgcgcagc
    10441 agcaccagcc ccggcgggtc gtccttgatc gtgccgatca cagccgcgcc gtcgccgagc
    10501 gggtgcgcgt gcagcgccgc cagcgccgcg tcggcctgct cggccgcaac gacggcgacg
    10561 agccttccct cgcaggcgac gtacagcggg tcgatgccga gcagctcgga cgccccgttg
    10621 acgaccggcc gcacgggcag cgcggcctcg tcgagcacca cggccacctg cgaggccacc
    10681 gccacctcgt tgcagatcgt ggcgacaccg ccgcgagtgg cgtcccggag caggcgcacc
    10741 ccgtcgccag ccgcgtcgag cagccccgcc gtgatctcgt gcagcggcgc ggtgtcggag
    10801 acgatgtcgg cggagatgtc gagctcgccg cgcgcgagca tcacggtgac gccgtggtcg
    10861 ccgatcggac ccgagacgag cacgacgtcg ccgggctgcg cagccgcggc cgagagcacg
    10921 acggggcgtt ccagcacccc cacgcctgcc gtgttgatgt agcagccgtc ggccttgccc
    10981 cgctcgacga ccttggtgtc gccggtgacg atctgcacgc cggcggcctc tgcggcagcg
    11041 gccatggacg ccacgatgcg ctgcaggtcg gcgaccggga agccctcctc gaggatgaaa
    11101 ccgcaggaca ggtacagcgg tcgtgcgccg cacatcgcga ggtcgttgac cgtgccgttg
    11161 accgcgaggt cgccgatgtc gcccccgggg aagaacagcg gggacaccac gtaggagtcg
    11221 gtcgtgaacg cgagccggcc ggtcacgccg ttcgaccacg tcggggcgag ctgggcgccg
    11281 tcctccaact gctccagcac cgggttgcgg aacgcatcga ggaacactgc ctcgatgagc
    11341 gtctgcgtcg cctttccgcc cgcgccgtgg gagagcgtga tccgctcctc tttgatcttc
    11401 gccttgcggg cgcgggcctt cgcgatccgg gccagcacct gctcctcacg ggtgaggtgc
    11461 tcggcgcccg cgtgggccgc gacagcctcc tccgcccagt tcgccctgac cgggatgttc
    11521 gagccgagat cctgggtcat gtccgggtcg cctccttgac gcgctcccgg ctgaaccggc
    11581 cgaagttgta gtacgcggca cacgctccct cggacgagac catgcaggtg ccgatcggag
    11641 tctcgggcgt gcacgcggtg ccgaagacct tgcactccca cggcttgagg acgcccttga
    11701 gcacctcgcc gcactggcag gccttcggat cggccacccg cacgcccggc acggagaaga
    11761 tccgctccgc gtcgaactgg gcgtagcgct ccctcacctg tagcgccgag tgcgagatga
    11821 agcccagccc gcgccactcg aagtgcggcc gcagctccat cacctcgccg atcacctgca
    11881 gcgccttggc gttgccgttc cacggcacca cgcgcgtgta ctggttctcg atctcgcagc
    11941 ggccctcctt cagctgcagc agcagctggt agaccgactg caggatgtcg agcggctcga
    12001 agccggcgca gaccagtggc ttgcccgcgt cgcgagcgat ccactcgtag ggccggcacc
    12061 cgatcacagt ggagacgtgg cccgggccga tgaagccgtc caggcgcagg tcgggggagt
    12121 cgaggatcgc cttgatcgcc gggatgatcg tgacgtggtt gcagaagatc gagaagttgt
    12181 cgagaccctc ggcggctgca cgcagcaccg tcatcgcggt ggacggcgcg gtggtctcga
    12241 acccgatggc catgaagacc acgcgcttgt cggggttctt ccgcgcgatc ttcaacgcgt
    12301 cgagcggcga gtagaccatc cggatgtccg cgccctcggc cttggcgtcg aagaacgacc
    12361 cggccccgcc cggcacccgc atcatgtcgc cgaacgcggt catgatcacg tcggggtcct
    12421 tggcgatcgc gatcgcgtcg tccacccgtc ccatcgggag cacgcagacc gggcagccgg
    12481 gaccgtgcac gagccggacc gactccggca ggtggtcctc gaggccgtgc t
//
LOCUS       DASRSC010000368         3292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_5886037,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000368 DASRSC010000000
VERSION     DASRSC010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5886037"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VFE39_17925"
     CDS             complement(<1..1035)
                     /locus_tag="VFE39_17925"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6260519.1"
                     /translation="MTAWTLITGGTVLSVDPAIGDLHRGDVLIENDVIREVAPRIDAP
                     EAQRIDATNRIVMPGLVDTHRHMWQAAVRQIAADWTIGEYVEHMLGGLGPKFSAEDVH
                     IADLLSGLEALNNGITTIFDWSHIVNTPEHADAAVEGLTESGIRGVYGYGLPRTPAPS
                     WYTADAARVRKTWFTSDDNRVTMALASLGPEFASMDEADVDLRLARELDLRTSLHLGC
                     GLLGGMRAITGMHERGLLGPDLIFVHCNTCTDEELRLIADTGGHVAISPRVEMQMGHG
                     YPATGRLIAAGVQPSLSVDVVSGVGGSLFAEMRGMLEAERGWQNYLTLQRGEWTSRLE
                     LRTRDAVRIAT"
     gene            complement(1032..2702)
                     /locus_tag="VFE39_17930"
     CDS             complement(1032..2702)
                     /locus_tag="VFE39_17930"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HET6260520.1"
                     /translation="MRAEGIYLNGRVWPGVRDGTGGDPTAIAVSGGRVVALDTDAVVR
                     DLAGPGTAIVDLGGRRVVPGLIDGHIHAVRAGASFDRELHWTGMPNVQSALKSIQGAT
                     ATCEPGEWIRAVGGWHPTQFTEGRSPTREELDGVAPDHPVYLQVLYEYSVLNTAAVQR
                     SGLDRMRQDPPGGVIERDADGRPTGRVSGLGAFSQCLAAMPRRTRDQERASTAAMMRE
                     LHAAGLTGLVDAGGFGMSPERYDSLFDLWRRDELSMRMRLYFSAVDPGHEPEQLEAWL
                     RHQQSRFGDDLLRVLGVGEVVHFGCHDFEGLEPFEICEEARADFLRITRATARRGWPM
                     QVHAVLDSSLDVILDVWEEVHAETPLTDLRFVIAHADRISPRNAARLAALGAGVVVDD
                     HLVFKAGASERVWGQEAIHRAPPLGELRRAGIPIAAGTDATRASSFSPWLALWWMVTG
                     RSLDGVRRRAPEHLLTREEALAAYTAGSAWMSFEEHERGNLFPGALADFAVLDADYLT
                     VDESAIPAIAADLTVVGGQPVFSTGAVADATRSAVSIPEPERAKELSR"
     gene            complement(2699..>3292)
                     /locus_tag="VFE39_17935"
     CDS             complement(2699..>3292)
                     /locus_tag="VFE39_17935"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HET6260521.1"
                     /translation="DRARAAVAESWRAAEGSSLTYLPFVARAVVEAIAKFPHVNASVG
                     DDELLVYNRVNLGIAVDLDADGLVVPVVRDADGKRLRAIARDVTRLARTARERKSTAD
                     DFAGGTFTISNPGPYGTLLTAAVINQPQVAILATDAVTPRPVAVEVPGGGYAVVVRPV
                     GNLALSFDHRAIDGGYAARFLREVKNGLEQRDWSAEL"
BASE COUNT          435 a         1190 c         1186 g          481 t
ORIGIN      
        1 ggtggcgatg cgcacggcgt cgcgcgtgcg cagctccagt cggctggtcc actcgccccg
       61 ctgcagcgtc aggtagttct gccacccgcg ctcggcctcc aacatgccgc gcatctcggc
      121 gaagaggctc ccgcccacgc cgctgacgac gtcgacgctg aggctcggct gcacccctgc
      181 ggcgatgagc cgcccggtgg ccgggtagcc gtggcccatc tgcatctcca cgcgcgggga
      241 gatcgcgacg tgccccccgg tatcggcgat gagccgcagt tcctcgtcgg tgcatgtgtt
      301 gcagtgcacg aagatcaggt ccggaccgag caggccgcgt tcgtgcatgc cggtgatggc
      361 gcgcatcccg ccgagcagcc cgcaccccaa gtgcagcgac gtccgcaggt cgagctcccg
      421 ggccagccgc aggtccacgt cggcctcgtc catggaggcg aactccggtc cgaggctggc
      481 gagcgccatc gtgacccggt tgtcgtcgga ggtgaaccag gtcttgcgga cgcgggcggc
      541 gtcggcggtg taccagctgg gggccggcgt gcgcggcagg ccgtagccgt agacgccgcg
      601 gatgccggac tcggtgagcc cttcgacggc tgcgtcagcg tgctcggggg tgttgacgat
      661 gtgcgaccag tcgaagatcg tcgtgatgcc gttgttgagc gcctccagcc cgctgagtag
      721 gtcggcgatg tgcacgtcct ccgcgctgaa cttcggcccg aggccgccga gcatgtgctc
      781 gacgtactcg ccgatcgtcc agtccgcggc gatctggcgc actgcggcct gccacatgtg
      841 gcggtgggtg tcgaccagtc cgggcatcac tatccggttg gtcgcgtcga tgcgctgggc
      901 ttccggcgcg tcgatgcggg gggccacctc gcggatgacg tcgttctcga tgagcacatc
      961 gccccggtgc aggtcgccga tcgcggggtc gacgctgagg acggttccgc cggtgatgag
     1021 ggtccacgcg gtcatcggga gagctccttc gctcgttccg gttcggggat ggagacggcg
     1081 ctgcgagtag cgtcggcgac agcgccggtg gagaagacgg gttgcccacc gacaacggtg
     1141 aggtcggcgg cgatggcggg gatcgcgctc tcgtcgacgg tgaggtagtc ggcgtcgagt
     1201 acggcgaagt cggcgagcgc accggggaac aggttcccgc gctcgtgctc ttcgaacgac
     1261 atccaggcgc tcccggccgt gtaggcggcc agcgcctcct ctcgcgtgag caggtgctcg
     1321 ggagcgcgcc gccgcacgcc gtccagtgag cggccggtga ccatccacca gagcgcgagc
     1381 catgggctga acgacgacgc gcgggtggcg tcggtcccgg cggcgatcgg gatccccgcg
     1441 cggcgcagtt cgccgagcgg cggcgcgcgg tggatggcct cctgccccca cacccgctcc
     1501 gacgcgcccg ccttgaacac aaggtggtcg tcgacgacga cccccgcgcc gagggccgcg
     1561 agacgtgcgg cgttgcgcgg gctgatccgg tccgcgtgcg cgatgacgaa ccgcaggtcg
     1621 gtgagcgggg tctccgcgtg gacctcctcc cagacatcga ggatgacgtc aaggctgctg
     1681 tcgagcactg catgcacctg catgggccaa ccgcggcggg ccgtcgcccg cgtgatccgg
     1741 aggaagtccg cgcgcgcctc ctcgcagatc tcgaacggct ccaggccctc gaagtcgtgg
     1801 cacccgaagt gcaccacctc gccgacgccc aggacgcgca gcaggtcgtc gccgaagcgg
     1861 ctctgctggt ggcgcagcca cgcctcgagc tgttcgggct cgtggcccgg gtccaccgca
     1921 gagaagtaga gccgcatccg catgctcagc tcgtcgcgcc gccacaggtc gaacaggctg
     1981 tcgtagcgct cggggctcat gccgaagccc cccgcgtcga ccagaccggt gagaccggcc
     2041 gcgtgcagct cgcgcatcat cgccgccgtg ctcgcccgct cctggtcccg ggtacgccga
     2101 ggcatggccg ccaggcactg ggagaacgca cccaggccgc tgacccggcc cgtcggccgg
     2161 ccgtcggcgt cgcgctcgat gacgccgccc ggtgggtcct gacgcatccg gtccaggccg
     2221 ctgcgttgca cggccgcggt gttcaggacg ctgtactcgt agaggacctg caggtagacg
     2281 gggtggtccg gggccacacc gtcgagctcc tcgcgggtgg gcgaccggcc ctcggtgaac
     2341 tgggtggggt gccagccgcc gacggcccgg atccactcgc cgggctcgca cgtcgcggtg
     2401 gcgccctgga tgctcttcag ggctgactgg acgttcggca tcccggtcca gtgcagctcg
     2461 cggtcgaacg acgcccccgc gcgcaccgcg tggatgtgcc cgtcgatcag accgggcacc
     2521 acgcggcggc caccgaggtc gacgatggcc gtgccggggc ccgcgaggtc ccgcaccacc
     2581 gcgtcggtgt cgagcgcgac cacgcgcccg cccgacaccg cgatcgcggt cgggtcaccc
     2641 ccggtcccgt cccgcacgcc cggccagacc cggccgttga ggtagatccc ctccgccctc
     2701 acagctctgc actccagtct cgctgctcca ggccgttctt gacctcccgg aggaaccgag
     2761 cggcgtaccc gccgtcgatc gcgcggtggt cgaacgacag cgcgaggttc cccaccgggc
     2821 gcaccaccac ggcgtagccg ccgccgggca cctccaccgc gaccgggcgc ggggtcaccg
     2881 cgtccgtggc gaggatcgcc acctgcggct ggttgatcac cgcggcggtg aggagggtgc
     2941 cgtacgggcc ggggttgctg atcgtgaagg tcccaccggc gaagtcgtcc gcagtgctct
     3001 tgcgctcccg cgcggtgcgg gccagccggg tgacgtcccg ggcgatcgcc cgcagccgct
     3061 tcccgtcggc gtcccggacg accgggacga cgaggccgtc cgcgtcgagg tcgaccgcta
     3121 tcccgaggtt gacccggttg tacacgagca gctcatcgtc gccgacgctc gcgttgacgt
     3181 gcgggaactt cgcgatcgct tccaccaccg cccgcgcgac gaacggcagg tacgtcaggc
     3241 tgctgccctc ggcagcccgc cagctctcgg cgaccgccgc cctggcccgg tc
//
LOCUS       DASRSC010000369         7923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2123832,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000369 DASRSC010000000
VERSION     DASRSC010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2123832"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFE39_17940"
     CDS             <1..553
                     /locus_tag="VFE39_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HET6260522.1"
                     /translation="AREGGTTVVLDATQSVGWFDADLSWADAVVAAAYKWLLSPRGAA
                     WMAMRPELEVVPHLAGWYAAADPWAGIYGLPMQVAQSARALDTSPAWYSHVGAAAVLP
                     WLAGLDRAAVHAHCVGLADGLRTALGMEPAGSAIVSVRTPRALERLTEAGVVASGRAG
                     AARLAFHLYNTEADMTRAAAALA"
     gene            complement(680..1411)
                     /locus_tag="VFE39_17945"
     CDS             complement(680..1411)
                     /locus_tag="VFE39_17945"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase E"
                     /protein_id="HET6260523.1"
                     /translation="MPADAPTILATSIGFSSRNRSQLDWAPGPIFPYAFELARTSGRP
                     KLCYIGTATGDAATYISAFYGAFADHDVTTSHLALFTMPNMENVRAHLLSQDVIWVGG
                     GSTANLLAVWRVHGLDRILRECWESGVVLGGVSAGSLCWHESGTTDSFGPELAAITNG
                     LAFLPYSNCAHCDSEVRRRPVFHELIGNGTIGAGYATEDGVGIHYRGTEFVEAVADRA
                     DVYGYRVERGDDGTVTETTITPRQL"
     gene            complement(1424..1921)
                     /locus_tag="VFE39_17950"
     CDS             complement(1424..1921)
                     /locus_tag="VFE39_17950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HET6260524.1"
                     /translation="MSGAPPTGPPRLTKATQDALQGALAAEHAALWCYSLIVAFLPAD
                     QLGQAGEDSDAHMTLRTAVEKSLTDAGTPPVSAQPAYSTPQPVVDGPSAAALAVVAET
                     DALAAWRAVVERTGNRGLRQYALDVLSAGTLRCARWRVVVNSPPAILPFPGRAGPAGP
                     SEPAR"
     gene            complement(1918..2397)
                     /locus_tag="VFE39_17955"
     CDS             complement(1918..2397)
                     /locus_tag="VFE39_17955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260525.1"
                     /translation="MRAPIGPATSPGISRRQVLAGLLLLPPALAGCSTAPVPQGPDPL
                     IALADAARTDAALATAAITASPNLADRVQPLVEARTAHAAALDAEVARLDPARPSTTP
                     ATTPAPAQGSATLAQVRAAVLASGEAAATAALDLPAERVGLVASVAACCNAYAEVLT"
     gene            2545..3087
                     /gene="rimP"
                     /locus_tag="VFE39_17960"
     CDS             2545..3087
                     /gene="rimP"
                     /locus_tag="VFE39_17960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET6260526.1"
                     /translation="MPSPRPAQDAGQPPGQLHGVLEPIVRDAGFELDQLDVRAAGRRH
                     TIKVVVDSDSGVGLDDIAHLSRAVSAELDLHEHLIGGSYNLEVTSPGVDRPLTGRRHW
                     RRAHLRQVAVRTHDGVTFTGRVGPAGEEAVTLLVDGELRGLRYADVAHAGVQVEFRPA
                     PESELQLLQGASEPPEEETA"
     gene            3084..4103
                     /gene="nusA"
                     /locus_tag="VFE39_17965"
     CDS             3084..4103
                     /gene="nusA"
                     /locus_tag="VFE39_17965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006236779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HET6260527.1"
                     /translation="MNVDIAALRTIEREKDIPFETVIEAIETALLTAYRHTDGHQSHA
                     RIDIDRKTGAVRVMAQELGPEGDVDSEWDDTPEGYGRIAATTARQVIVQRLRDAEHER
                     TYGEFAAKEGEIVAGVIQRDARANARGVVIVQLSGQTEAVLPQGEQVPGERYEHGSRI
                     RCYVLGVTRGMRGTQITLSRTHPNLVRKLFALEVPEIVDGSVEIVAVAREAGHRSKIA
                     VRSTVPGVNPKGACIGPVGARVRGVMSELAGEKIDIIDWSEDPATFVGNALSPSKVVS
                     VTVLDARTRTARVVVPDFQLSLAIGKEGQNARLAARLTGWRIDIRSDADPRGAVDAPE
                     SESVG"
     gene            4244..4576
                     /locus_tag="VFE39_17970"
     CDS             4244..4576
                     /locus_tag="VFE39_17970"
                     /inference="COORDINATES: protein motif:HMM:NF016204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET6260528.1"
                     /translation="MGCRTRSKADGLLRVVVVDGVLTPDPRRRLPGRGAWLHPAPECL
                     DRAERRSAFPRALRVPGPLDTGAVRVRIEAGGRSEHANEAMRMGSTASGARERPGSVT
                     RGSKVDPS"
     gene            4670..7777
                     /gene="infB"
                     /locus_tag="VFE39_17975"
     CDS             4670..7777
                     /gene="infB"
                     /locus_tag="VFE39_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013228121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET6260529.1"
                     /translation="MAGKARVHELAKELGVSSKQVLSKLQDLGEYVKSPSSTVEAPVA
                     RKLRESMSNGNDSGGRRSGGGRPRPGGGTPGPSAVPSRPAQPSGGSVPGPGRPGPQQP
                     SAPAASAPAASAPAPSSTGSQDRPRPTGPRPGPRPGPAATPAPPERSEPQQPAAQAPA
                     ASLPSQPATRPAQPDPGRPQPGQAQPGQGPATPGQQRPAAADAGGVVPPRPSGPRPGP
                     RTPRVGNNPFGVGSGAPPRPAAPRPGPPAPGQPPAQGGQGQGQGAGPSQGGPSQGAPR
                     PGGPRPGAPRPGEPGRAGPRPPSPGNMPPRPNPGMMPARPAAGRGGPGGPGGRGGPGG
                     RPAGPGGRPGGPGGRPGGGGFRPGGGGPGGPGGGGAPAGGGFRSGPGGGGRPGGGGGR
                     GRGGAAGAFGRPGGPARKGRKSKRQKRQEFDSMQAPSVGGVRLPKGHGETIKLPRGAS
                     LTDFADKINANPAALVQVLFHLGEMVTSTQSVSDEILELLGSEMNYAVQVVSPEEEDR
                     ELLDSFSISYGEDAGGEEDLVIRPPVVTVMGHVDHGKTRLLDTIRKANVMEGEAGGIT
                     QHIGAYQVVTELEGYERPITFIDTPGHEAFTAMRARGAKSTDIAVIVVAADDGVMPQT
                     VEAINHAQAADVPIVVAVNKIDKEGANPAKIRQQLTEYGLVAEEYGGDTMFVDISARE
                     NINIDQLLEAILLTADASLDLRANPDMEAQGVAIEAHLDRGRGPVATVLVQRGTLRVG
                     DSVVAGDASGRVRRMFDEHGDDVIEAYPSRPVQVIGFTSVPGAGDTFLVVDEDRVARQ
                     IADRRRARERNAELASRRKRVSLEDLDAALKETNQLNLIIKGDNSGTVEALEDALLQL
                     EVGEDVELRVIHRGVGGITEGDINLAIADNVIVLGFNVRAEGKATELANREGVEIRYY
                     SVIYQAIEDIEQALKGMLKPEFEEVELGRAEVREVFKSSRVGTIAGCLVMSGTIRRNA
                     RGRLLRDSVVIADNLPISSLRRFKDDVVEVREGFECGYTLGNYSDIKVGDVIETFEMR
                     EKPRS"
     gene            7847..>7923
                     /locus_tag="VFE39_17980"
     CDS             7847..>7923
                     /locus_tag="VFE39_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020646010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 family protein"
                     /protein_id="HET6260530.1"
                     /translation="MFVGALELDVLLGDVHSLKQKRSVVR"
BASE COUNT         1136 a         2743 c         2962 g         1082 t
ORIGIN      
        1 ggcccgcgag ggcggcacga cggtggtgct ggacgcgacc cagtccgtcg gctggttcga
       61 cgccgacctg agctgggccg acgccgtggt ggccgcagcg tacaagtggc tgctcagccc
      121 gcgcggggcc gcgtggatgg cgatgcggcc cgagctggag gtcgtaccgc acctggcggg
      181 gtggtatgcg gccgccgacc cgtgggcggg catctacggc ctgccgatgc aggtggcaca
      241 gagtgcgcgg gcgctcgaca cctcccccgc ctggtacagc catgtcgggg ctgcggccgt
      301 cctgccgtgg ctcgccggcc tggaccgggc ggccgtgcac gcgcactgtg tcggcctggc
      361 cgacgggctc cggacggcgt tggggatgga gcccgccggg tctgcgatcg tgtccgtacg
      421 gaccccgcgc gcgttggagc gcctgacgga ggccggggtc gtggcgagcg gccgcgccgg
      481 agcggcgcgg ctggcgttcc acctctacaa caccgaggcc gacatgacgc gggcggccgc
      541 cgccctcgct tgaccccatc tcggggcgga accccacgtc atcaccgtgg cgccgggggg
      601 ttcgccggca ggccggcgtc ggtggacggc gtcttcagcc tggtacgcag ccgagcaccc
      661 aggccccggc tgctcgacgt cagagctgcc ggggggtgat cgtcgtctcg gtcaccgtgc
      721 cgtcgtcgcc gcgttcgact cggtagccgt agacgtctgc gcgatccgcg accgcttcga
      781 cgaactcggt gccccggtag tggattccga cgccgtcttc ggtggcgtaa ccggcgccga
      841 tcgtgccgtt gccgatcagt tcgtggaaaa ccggccgacg cctcacctcg ctgtcgcagt
      901 gcgcgcagtt cgagtacggc agaaaggcca ggccgttggt gatcgcagcc agctccggcc
      961 cgaacgagtc ggtggtgcca ctctcgtgcc agcagagcga cccggccgag acgccgccga
     1021 ggaccacgcc ggactcccag cactcgcgca ggatccggtc cagcccgtgt acccgccaga
     1081 ccgcgagcag gttcgcggta ctgccgccgc cgacccagat gacgtcctgg gagaggaggt
     1141 gcgctcggac gttctccatg ttcggcatcg tgaacagggc cagatgagac gtcgtgacgt
     1201 cgtggtcggc gaatgcaccg tagaaggcgc tgatgtaggt ggccgcgtca ccggtcgcgg
     1261 tcccgatgta acagagcttc gggcgtccgg acgtcctggc caactcgaag gcgtagggga
     1321 agatcgggcc cggcgcccag tccagctggc tccggttccg tgaggagaag ccgatcgagg
     1381 tggcgaggat ggtgggtgca tcagcaggca tgccctgatc gaatcatcgg gccggttcgg
     1441 acggacccgc cgggccagca cggccgggga agggcaggat cgcgggcggc gagttgacga
     1501 ccacccgcca ccgagcgcag cggagtgtgc ccgcgctgag gacgtcgagt gcgtactggc
     1561 gcagcccgcg gttaccggtg cgctccacca ccgcgcgcca tgcggcgagc gcgtcggtct
     1621 cggcgacgac cgccagcgcg gccgcggagg gcccgtcgac gacgggctgc ggggtgctgt
     1681 aggcgggttg cgccgagacg ggaggcgtcc cggcatccgt gagtgacttc tcgaccgccg
     1741 tgcgcaacgt catgtgggca tccgagtctt caccggcctg tccgagctgg tcggccggca
     1801 ggaaggcgac gatgagtgag tagcaccaca gggcggcgtg ttctgcggcg agcgcgccct
     1861 gcagcgcgtc ctgggtcgcc ttcgtgagtc ggggcggtcc ggtcggaggt gccccgctca
     1921 cgtgagcacc tctgcgtagg cgttgcagca cgccgccacc gatgcgacga gccccacgcg
     1981 ctcggcgggc agatcgagtg cggcggtggc cgcggcctcg ccggatgcga gcaccgccgc
     2041 gcgcacctgt gccagggtgg cggacccctg cgccggagcg ggcgtcgtgg ccggcgtggt
     2101 ggacggcctc gcggggtcga gccgggcgac ctcggcgtcg agggcggcgg cgtgcgcggt
     2161 gcgggcctcg acaaggggct gtacgcggtc ggcgaggttc ggcgacgcgg tgatcgccgc
     2221 cgtggccagc gcggcgtccg tgcgtgcggc atcggcgagc gcgatcagcg ggtcgggccc
     2281 ctggggcacg ggggcggtcg agcagccggc cagcgcgggc gggaggagca gcagtccggc
     2341 gagcacctga cgacgggaga ttccgggcga ggtcgccggc ccgatcggtg ctctcacgac
     2401 ttctcctcac cctgctcgcc gcgggcacat cctgccaggc cgcggccgtg gcccggcgcg
     2461 ataccctggc aaactcaccg caagcgccag cggcatccgc cgcagtgtcg cgtaggcgga
     2521 gcacgacaga gcaggagtca tccgatgccc tccccccgcc ccgcgcagga tgccgggcag
     2581 ccgcccggac agctgcacgg cgtgctggaa ccgatcgtcc gtgatgccgg attcgagctg
     2641 gaccagctcg acgtccgtgc cgcgggccgc cgtcacacca tcaaggtcgt ggtggactcc
     2701 gactccggcg tcgggctgga cgacatcgcg catctgtccc gggccgtctc ggccgagctg
     2761 gacctgcacg agcacctcat cggcggctcc tacaacctcg aggtcacgtc ccctggcgtg
     2821 gaccggccgc tgaccggtcg ccggcattgg cgccgggccc acctgcgtca ggtcgccgtc
     2881 cgcacccacg acggggttac gttcaccggc cgggtcgggc cggccggcga ggaggctgtc
     2941 acgctgctcg tcgacggcga gctgcggggg ctgcgctacg ccgacgtggc acacgccggg
     3001 gttcaggtgg agttccggcc cgcacccgaa tcggagctgc agctgctgca gggggcgtcc
     3061 gagccacctg aggaggagac ggcatgaacg tcgacatcgc ggcgctgcga accatcgagc
     3121 gcgagaagga catcccgttc gagacggtca tcgaagcgat cgagaccgcc ctgctcaccg
     3181 cctacaggca caccgacggc caccagtcgc acgcccgcat cgacatcgac cgcaagacgg
     3241 gcgctgtccg cgtgatggcg caggagctgg gtcccgaggg tgatgtcgac tccgagtggg
     3301 acgacacccc cgagggctac ggccggatcg cggccaccac cgcgcgccag gtgatcgtcc
     3361 agcgcctgcg cgacgcagag cacgagcgga cctacggcga gttcgcggcg aaggaaggcg
     3421 agatcgtcgc gggggtcatt cagcgcgacg cgcgggccaa cgcgcgcggg gtggtgatcg
     3481 tgcagttgag cggccagacc gaggccgtgc tgccgcaggg ggagcaggtg cccggcgagc
     3541 ggtacgagca cggttcgcgg atccggtgct acgtgctcgg agtgacgcgg ggaatgcgcg
     3601 ggacgcagat cacgttgagc cggacgcatc ccaatctcgt acggaagttg ttcgcgctcg
     3661 aggttccgga gatcgtcgac ggctcggtgg agatcgtcgc ggtggccagg gaggccgggc
     3721 accggtcgaa gatcgccgtc cggtcgaccg taccgggggt caatccgaag ggtgcgtgca
     3781 tcggccccgt cggggcgcgg gtgcgcggcg tgatgagcga gcttgccgga gagaagatcg
     3841 acatcatcga ctggtcagag gacccggcga cgttcgttgg caatgccctg tcaccgtcga
     3901 aagtggtgtc ggtcacggtt ctcgatgcgc gcaccagaac cgcgcgcgtg gtggtgcccg
     3961 acttccagct gtcgctggcc atcggcaagg aaggccagaa tgcccggttg gcggcccggc
     4021 tcacgggctg gcgcatcgat atccgtagcg acgccgaccc ccgaggggcc gtcgacgcac
     4081 ccgagtcgga gtcggtcggc tgagtcgcaa gggacccagc gaaggcggaa cgcgcaacgg
     4141 tgctgcgcta cactccacct cggcccacaa ctcgggttcg gctcccgccc gtccggcgcc
     4201 cgctgcggat ctgcgatccg cggcaggacc tcttcggacc tgcatcggtt gccggacccg
     4261 gtcgaaggcc gacggtctgt tgcgggtcgt cgtggtggac ggggtgctca ccccggaccc
     4321 gcggcggcgg ctccccggac gaggtgcatg gttgcacccg gctccggagt gcctggaccg
     4381 tgccgagcgg cgctcggcgt tcccgcgggc gctacgcgtc ccgggtccgc tggacaccgg
     4441 tgcggtacga gtcagaatcg aggctggggg tcggagcgag cacgcgaacg aagcaatgcg
     4501 gatgggcagt accgcgagcg gggcccggga gagacccggg tccgtcacac gaggaagcaa
     4561 ggtcgacccg tcatgagcca gtcgtgaaga tcgcaccatg aacgtgcttc gacactaggt
     4621 tcgaggtcga gcggaaaccg ccgccggcct ccgataaaca aggagtgcag tggcaggcaa
     4681 ggcccgcgtg cacgagctcg caaaggagct cggcgtcagc agcaagcaag tcctgagcaa
     4741 gttgcaggat ctcggcgagt acgtgaagtc cccgtcctcg accgtcgagg cccccgtggc
     4801 ccgcaagctt cgtgaatcca tgtccaacgg caacgactcg ggtggtcgtc gttccggtgg
     4861 cggcaggccg cgccctggtg gcggcactcc gggtccgtcg gccgtgccgt cgcgcccggc
     4921 gcagccgtcg ggtggttcgg tccccggacc gggacgcccg gggccgcagc agccgtccgc
     4981 cccggcggcg tcggccccgg ccgcatccgc tccggccccg tcctcgacgg ggtcgcagga
     5041 ccgtcctcgg cccaccggcc cgcgcccagg tcctcggccc ggccccgcgg ccactccggc
     5101 cccgccggag cgcagcgagc cgcagcagcc cgccgcgcag gccccggccg cgtcgctgcc
     5161 gtcgcagcct gcgactcgcc ccgcgcagcc cgatcccggt cggccgcagc ccgggcaggc
     5221 ccagccgggt cagggcccgg cgacgcctgg tcagcagcgg cccgccgctg ccgacgcagg
     5281 tggggtcgtc ccgccgcgtc cgagcggccc gcgtcccggc ccgcgcacgc cgcgggtggg
     5341 taacaacccg ttcggtgtcg gttccggcgc cccgccgcgc ccggccgctc cgcgtcccgg
     5401 cccgcccgca cccgggcagc cccccgcaca gggtggccag ggtcaggggc agggcgctgg
     5461 tcccagccag ggcggtccca gccagggcgc ccctcgtccc ggcggtccgc gtcccggggc
     5521 cccgcgtccc ggtgagcccg gccgcgccgg cccgcgcccc ccgtcgcccg gcaacatgcc
     5581 tccgcgcccg aaccccggca tgatgccggc gcgtccggct gccggacgtg gtggtccggg
     5641 tggtcccggt ggccgtggtg gtcccggtgg tcgtcctgcc ggtcccggcg gtcgtcccgg
     5701 tggtcccggc ggtcgtcccg gcggtggcgg cttccgtccc ggcggcggtg gtcccggtgg
     5761 tcctggtggt ggcggcgctc cggcaggtgg cggtttccgc agcggtcccg gtggcggtgg
     5821 ccgtccgggt ggcggcggcg gtcgcggtcg tggtggagcc gcaggtgcct tcggccgtcc
     5881 cggtgggccg gcccgcaagg gtcgcaagtc gaagcggcag aagcgccagg agttcgactc
     5941 gatgcaggcc ccctcggtcg gcggcgtccg cctgccgaag ggccacggag agacgatcaa
     6001 gctcccgcgt ggtgcctcgc tcaccgactt cgccgacaag atcaatgcca accccgccgc
     6061 gctggtgcag gtgctgttcc acctcggtga gatggtcacc tccacgcagt cggtgtccga
     6121 cgagatcctc gagctgctcg gcagcgagat gaactacgcc gtgcaggtcg tcagcccgga
     6181 ggaggaggac cgcgagctgc tcgacagctt ctccatctcc tacggcgagg acgccggtgg
     6241 cgaggaggac ctggtcatcc ggccgccggt cgtcaccgtc atgggtcacg tcgaccacgg
     6301 caagacgcgc ctgctcgaca cgatccgcaa ggccaacgtc atggagggcg aggcaggcgg
     6361 catcacccag cacatcggtg cctaccaggt ggtcaccgag ctcgagggct acgagcggcc
     6421 catcactttc atcgacaccc cgggccacga ggcgttcacc gccatgcgtg cccgcggtgc
     6481 caagtcgacg gacatcgcgg tcatcgtggt ggcggccgac gacggcgtga tgccgcagac
     6541 ggtggaggcg atcaaccacg cccaggcggc cgacgtgccg atcgtggtcg cggtcaacaa
     6601 gatcgacaag gagggggcca accccgccaa gatccgtcag cagctcaccg agtacgggct
     6661 ggtcgccgag gagtatggcg gcgacacgat gttcgtcgac atctcggcgc gcgagaacat
     6721 caacatcgac cagctgctcg aggcgatcct gctcaccgcc gacgcctccc tcgacctccg
     6781 ggccaacccc gacatggagg cgcagggcgt cgcgatcgag gcccacctgg accgcggtcg
     6841 cggtccggtg gccacggtgc tggtgcagcg cggcacgctc cgggtcggcg actcggttgt
     6901 ggcgggcgac gcctccgggc gcgtccggcg gatgttcgac gagcacggcg acgacgtcat
     6961 cgaggcgtac ccgtcgcgtc cggtgcaggt catcgggttc acctcggtgc caggcgcggg
     7021 cgacacgttc ctcgtcgtcg acgaggaccg ggtggctcgc cagatcgccg accggcgccg
     7081 ggcccgcgag cgcaacgccg aactggcgtc gcgtcgcaag cgggtcagcc tggaggacct
     7141 ggacgccgcg ctcaaggaga ccaaccagct caacctcatc atcaagggcg acaactcggg
     7201 aacggtcgag gccctcgagg acgcgttgtt gcagctggag gtcggcgagg acgtggagct
     7261 gcgggtgatc caccgcggtg tcggtggcat caccgagggc gacatcaacc tcgccatcgc
     7321 cgacaacgtc atcgtcctgg gcttcaacgt ccgggccgag ggcaaggcga cggagctggc
     7381 caaccgcgag ggcgtggaga tccggtacta ctcggtgatc taccaggcca tcgaggacat
     7441 cgagcaggcg ctcaagggca tgctcaagcc ggagttcgaa gaggtcgagc tgggccgcgc
     7501 ggaggtccgc gaggtcttca agtcctcccg ggtcggcacg atcgccggtt gcctcgtgat
     7561 gtccggcacg atccggcgga acgcgcgggg ccggctgctg cgcgacagcg tggtgatcgc
     7621 ggacaacctc ccgatcagct cgctgcggcg gttcaaggac gacgtggtcg aggtccgcga
     7681 gggcttcgag tgcggttaca ccctcggtaa ctacagcgat atcaaggtcg gcgacgtcat
     7741 cgagaccttc gagatgcgcg agaagccgag gagctgacgc gacggggggg accggctccg
     7801 gccggtcccc ccaccggcgt ctcaccagga agatcgtgga gaccccatgt tcgtcggagc
     7861 gctggagctg gacgtcctgc tgggggacgt ccattccctg aagcagaagc ggtcggtggt
     7921 ccg
//
LOCUS       DASRSC010000370         5958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_649735,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000370 DASRSC010000000
VERSION     DASRSC010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649735"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1559)
                     /locus_tag="VFE39_17985"
     CDS             complement(<1..1559)
                     /locus_tag="VFE39_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009157112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HET6260531.1"
                     /translation="MSAPGAGPADARPDAAAGGRLSHRQILTIFAGLMIGMFLAALDQ
                     TIVATSIRTIADDLGGLSLQAWATTAYLITATITTPLYGKLSDLFGRKPLFLTAISIF
                     LVGSAACTFATSMYQLAAFRGIQGLGAGGVFSLALTIIGDIVAPRERARYQGYFVAVF
                     GTSSVLGPVAGGFFAGHADILGITGWRWVFLINVPLGIIALIVVTKVLNVPHIKRKHR
                     IDWLGAIALTVALVPLLIVAEQGRRWGWHSTSALVCYAVGVVGIGAFLVLERRIGDDA
                     LLPLRFFSNGVFGWGSIAAFVAGIGMFGVLALLPLYLQIVKGSSPTKAGLQTLPLVLG
                     IMSMSVFSGQLISRTGRYKVWPVIGLSLMIVGAALLSRVDVDTPYWRTALIMIVFGWG
                     LGSNMQPLTLAVQNAMPPRDMGVATASATFFRQMGGTLGTAVFLSVLFSELGGRVADN
                     FRTASATPQFQAAVADPAVVAHPANEPILEAVQGGTEPSLEDSSFLSAADPVLSRPIL
                     DGFADSMSVVFV"
     gene            complement(1570..2076)
                     /locus_tag="VFE39_17990"
     CDS             complement(1570..2076)
                     /locus_tag="VFE39_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6260532.1"
                     /translation="MPTQDELAIADAVGSQLIRLVRLVERKQVQYQAEHPDAVERATY
                     HLLVHLVKDGARRAGSLAEAVHSDPSTISRQIGHLVRLGLVERTADPVDGRATLLAAT
                     EEGRRVFEENRRIRNERIAEMLERWSVDDRRALSELLTRFTSDFERSVPRPAEHTAAT
                     SDVAAVRS"
     gene            complement(2234..3448)
                     /locus_tag="VFE39_17995"
     CDS             complement(2234..3448)
                     /locus_tag="VFE39_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HET6260533.1"
                     /translation="MRDAVICEPLRTPVGGFGGSLRDVPAHELAAVVIRGLLDRTGLP
                     PESVDDVLLGHCYPTMDAPAIGRVAALDAGLPVTVNGLQIDRRCGSGLQSVLYAAMQV
                     QAGASELVLAGGAESMSGAPFYSTSMRWGAKGGPGVMLHDALARGRVTAGGINFPVPG
                     GMLETAENLRREYSIPREEQDEFAVRSHHRAAAARDEGRFADEIVPVTVKGRKGDTVV
                     DTDEHIRADSSLEKLTKLRPVLGRDDPSATVTAGNASGQNDGASICVVTHPAKAAELG
                     LRPLVRLVSWGVGGVPPATMGIGPVPAVAKALDSAELTLADIDLIELNEAFAAQVLAC
                     TREWKLAPEDFDRLNVNGSGISLGHPVGATGGRILATLTREMDRRAVRYGLETMCIGG
                     GQGLAAVFERVA"
     gene            complement(3509..3961)
                     /locus_tag="VFE39_18000"
     CDS             complement(3509..3961)
                     /locus_tag="VFE39_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HET6260534.1"
                     /translation="MRVFNGVGELRAAVGTHLGVSDWVTIDQSHIGMFADATGDHQWI
                     HVDEDRAKEGPFGTTIAHGFLTLSLLPVLTWQIYRVENVKMGINYGLNRVRFTSPVPV
                     GSKVRGSIELVDISDVDGGVQVTNKVIVEIEGSERPALVAEWLTRQHL"
     gene            4103..5047
                     /locus_tag="VFE39_18005"
     CDS             4103..5047
                     /locus_tag="VFE39_18005"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6260535.1"
                     /translation="MGTAADRAPPQRPEGAWHEAVLYSSSAHLVERLAPQVAAAVSAG
                     DPVVAVLDETTCEQLRKTLGPDSDAVDFQDPHEVHRMPAFTVAVRWARTSRRITNPDG
                     RALVVGQQVDEIDRGPQHWARLDIGVNVATAGLPITVLCPFADDVAHLPTVHATHPLL
                     STAKGSEPSPGYRQPEESVLDYPPPPPPDLGPPVAELTFVPDDLVELRHLVVRVAGAV
                     GLDTDRVADTVLAVNELASNSVEHGPGWGRLRLWSDAGVIAEVADGGRLTLPFPGMTM
                     PPPGGARGRGLWLASELSDSLEVWSESGSTVIRVQMSG"
     gene            5096..5872
                     /locus_tag="VFE39_18010"
     CDS             5096..5872
                     /locus_tag="VFE39_18010"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HET6260536.1"
                     /translation="MDAVARFAELVGHPDPPLDRAALALAAGADAELDPARWLAELDR
                     LADGVTSLDALVHRLFVKEAFAGNVHDYYDPRNSLLPHVLTRKLGIPITLAIVTIEVG
                     RRAGIALEGVGMPGHFLVRAVGTHTVLDVFHGGIELDAADCEALFRSSSGAGPDVSFG
                     PHLLATAPTRSILLRMLENLRLVYRIRQRPGDLEWVVRMRLALPGAGLADLLELATAL
                     GDQARWLEGARLLEEQLPTASAAGAERLRTAARTLRAHLN"
BASE COUNT          900 a         2133 c         2056 g          869 t
ORIGIN      
        1 acgaacacca cgctcatgga gtccgcgaat ccgtcgagaa tcggtcggct caggacgggg
       61 tcggctgccg acaggaacga ggagtcctcg agcgacggct cggtgccacc ctgcacggcc
      121 tcgaggatcg gctcgttggc cgggtgggct accaccgccg gatcggcgac ggccgcctgg
      181 aactgcgggg tggcggatgc cgtccggaag ttgtcggcca cccgcccccc cagctcgctg
      241 aacaggactg acaggaagac cgccgtgccc agcgtgccgc ccatctggcg gaagaacgtg
      301 gcggaggcgg tggcgacgcc catgtcccgc ggcggcattg cgttctgcac ggcaagggtg
      361 agcggctgca tgttgctgcc gagaccccag ccgaacacga tcatgatcag ggcggtccgc
      421 cagtaggggg tgtcgacgtc gacgcgtgag agcagcgcag ccccgacgat catcagcgag
      481 agtccgatga ccggccacac cttgtaccgc ccggtgcgcg agatcagctg accggagaag
      541 accgacatcg acatgatgcc cagcaccagc ggcaaagtct gcaggccggc cttcgtgggc
      601 gaggagccct tgacgatctg caggtacagc ggcagcagcg ccagcacgcc gaacatgccg
      661 atgcccgcga cgaacgcggc gatcgagccc cagccgaaca cgccgttgct gaagaaccgc
      721 aacgggagca gcgcgtcgtc gccgatccgt cgctcgagca ccaggaacgc gccgatgccg
      781 acgacgccga cggcgtaaca gacgagcgcg gacgtggagt gccatcccca cctgcggccc
      841 tgttcggcga cgatcaggag tggcacgagc gccactgtca gggcaatcgc ccccagccag
      901 tcgatgcggt gcttccgctt gatgtgcggg acgttgagca ccttcgtgac gacgatcagc
      961 gcgatgatcc cgagcgggac gttgatcagg aagacccagc gccacccggt gattcccagg
     1021 atatcggcgt ggccggcgaa gaatccgcct gccactggcc cgagcacgct ggacgtgccg
     1081 aagaccgcca cgaagtagcc ctggtacctg gcgcgttcgc gcggcgcaac gatgtcgccg
     1141 atgattgtca gggccagaga gaacacgccc cccgctccca gtccctggat cccgcggaag
     1201 gctgcgagct ggtacatcga cgtggcgaag gtgcaggccg ccgagccgac gaggaagatc
     1261 gagatcgcgg tgaggaacag cggcttgcgc ccgaagaggt ccgacagctt gccgtagagc
     1321 ggcgtcgtga tcgtcgccgt gatcaggtag gcggtggtcg cccaggcctg caggctgagc
     1381 ccgccgaggt cgtcggcgat cgtcctgatc gaggtggcca cgatcgtctg gtcgagcgca
     1441 gcgaggaaca tgccgatcat cagcccggcg aagatcgtca ggatctgccg gtggctcagt
     1501 cgacctcctg ccgcggcgtc gggccgggca tcggcgggcc cggctccagg cgcggacacg
     1561 aggctctcct caagaccgga cggctgccac gtcactggtg gcggcggtgt gctcggccgg
     1621 acgtggcacc gacctctcga aatcgctggt gaagcgggtc agcagctcgg acagcgcacg
     1681 ccggtcgtcg accgaccacc tctcgagcat ctccgcgatc cgctcgttgc ggatacgccg
     1741 gttctcctcg aacacccgcc tgccctcctc ggtggcggcc agcagcgtcg cccggccgtc
     1801 caccgggtcg gcggtgcgct cgaccagccc caggcgcacc aggtggccga tctggcggct
     1861 gatggtcgac gggtcggagt gcacggcctc ggcgagggac cccgcgcggc gcgccccgtc
     1921 cttcaccagg tggacgagca ggtggtaggt ggcccgctcc acggcgtccg gatgctcggc
     1981 ctggtactgc acctgcttgc gctcgaccag gcggaccagc cggatgagct ggctgccgac
     2041 cgcgtcggca atggcgagct cgtcctgggt cggcacggtg tagctccctg cggattatgt
     2101 cggcttgcat gtggcaagca aaagcttgtc tcatacaagc atgtaccggg ccctggcagt
     2161 tcggcaaccg aagccggtgt gatatgcgct gtccgcagtg tgagcggcgg ccggcgaagg
     2221 ccgtcagggc cggtcaggca acgcgttcga agaccgcagc gaggccctgg cctcctccga
     2281 tgcacatcgt ctcgaggccg tagcgcaccg cacggcggtc catctcccgc gtgagcgtgg
     2341 ccaggatccg ccctccggtg gcgcccacgg ggtgaccaag ggagatgcct gagccgttga
     2401 cgttgagccg gtcgaagtcc tccggcgcga gcttccactc gcgggtgcag gccaggacct
     2461 gagcggcgaa cgcctcgttc agctcgatca ggtcgatgtc ggccagggtg agctcggcgg
     2521 agtccagggc cttcgcgacg gcgggcacgg gcccgatgcc catcgttgcg ggcggcaccc
     2581 cacccacgcc ccacgacacg agcctgacca ggggccgcag cccgagctcc gctgccttgg
     2641 ccgggtgggt gaccacgcag atggatgcgc cgtcgttctg cccgctggcg ttgcccgcgg
     2701 tgacggtcgc cgacggatcg tcccgcccca gtaccggtcg cagcttggtc agcttctcca
     2761 gcgacgagtc ggcgcggatg tgctcgtcgg tgtccaccac ggtgtcgccc ttccggccct
     2821 tcaccgtgac cggcacgatc tcgtcggcga accggccctc gtcccttgcg gcggcagcgc
     2881 ggtggtgcga gcggaccgcg aactcgtcct gctcctcgcg cgggatcgag tactcccggc
     2941 gcaggttctc cgcggtctcg agcatgccgc cgggtaccgg gaagttgatc ccgcccgccg
     3001 tgacacggcc acgggcgagt gcgtcatgca gcatcacgcc cggcccgccc ttcgcgcccc
     3061 agcgcatgga cgtggagtag aacggcgccc ccgacatcga ctcggccccg cccgccagca
     3121 ccagctccga cgcaccggcc tgcacctgca tcgccgcgta caggacggac tgcagcccgg
     3181 aaccgcagcg ccggtcgatc tggaggccgt tgacggtgac cggcaggccg gcgtccagcg
     3241 cggcgacgcg gccgatcgcc ggcgcgtcca tcgtcgggta gcagtgcccg agcaggacgt
     3301 cgtcgaccga ctccggcgga agcccggtgc ggtccagcag gccgcggatc accacggctg
     3361 ccagctcgtg ggcaggtacg tcccgcaacg agccgccgaa gccgcccacc ggggtccgta
     3421 gcggctcgca gatgaccgcg tcgcgcatca ggtcgtctcc ttcgtcgtcc ctgtcctaca
     3481 cgtactgccg gcatgtactg ccggagtcct acaggtgctg ccgggtcagc cactccgcca
     3541 cgagcgcggg gcgttccgaa ccctcgatct ccacgatgac cttgttggtg acctgcaccc
     3601 cgccgtccac gtcgctgatg tcgacgagct cgatgctgcc cctgaccttg ctcccgacgg
     3661 gcaccggcga ggtgaacctc acccggttca ggccgtagtt gatgcccatc ttgacgttct
     3721 cgacccggta tatctgccag gtcagcacgg gcaggagcga gagcgtcagg aacccgtgcg
     3781 cgatcgtggt gccgaacggg ccctccttcg ctctgtcctc gtcgacgtga atccactggt
     3841 gatcgcctgt ggcatctgcg aacatgccga tatgggactg gtcaatcgtg acccagtcgc
     3901 tcaccccgag gtgcgtgccc accgcagccc gcagctcccc gacaccgttg aagacgcgca
     3961 tcgccgctcc tgatccctcc gctgtagcac cgaacgatct cggctgccga tccaccttgc
     4021 catacgggcc ccgggtgatt cgcgatgctc cgagcgggta cgcgcttggc gtcggcactc
     4081 gaagcagggg acggggacgg ccatgggaac cgcagcggac agagcgccac cgcagcgacc
     4141 cgagggtgcg tggcacgagg ccgtgctgta ctcctcgtcc gcccaccttg tcgagcggct
     4201 ggctccgcag gtcgcagcag ccgtgtcggc cggcgacccg gtcgtggcgg tgctcgacga
     4261 gaccacttgc gagcagctgc ggaagacgct gggccccgac tccgacgcgg tggacttcca
     4321 ggacccccac gaggtgcacc ggatgcccgc cttcaccgtc gccgtgcggt gggccaggac
     4381 cagcagacgc atcaccaacc ctgacggccg tgcactcgtc gtcggccagc aggtggacga
     4441 gatcgaccgc gggccacagc actgggcgcg cctggacatc ggcgtgaacg tggcgaccgc
     4501 cgggctgccg atcacggtgc tctgcccgtt cgccgacgat gtcgcccacc tgcccaccgt
     4561 gcacgccacc cacccgctgc tgagcacggc gaagggctcg gagccgagcc ccgggtaccg
     4621 gcagccggag gagtcggtac tggactaccc gccgccaccg ccacccgacc tcggcccgcc
     4681 ggtcgcggag ctcaccttcg tcccggacga cctggtggag ctccgtcacc tcgtcgtccg
     4741 ggtggccggc gccgtcggcc tcgacaccga ccgcgtggcg gacacggtgc tcgcggtgaa
     4801 cgagctcgcc tcgaacagcg tggagcacgg gccgggatgg ggccggctgc gtctgtggag
     4861 cgacgcaggc gtcatcgccg aggtcgccga cggcggtcgg ttgaccctgc cgttcccggg
     4921 catgacgatg ccgccaccgg ggggcgcccg tggccgcggg ctctggctcg cctcggagct
     4981 gtccgactcg ctcgaggtgt ggagcgaatc cggcagcacc gtcatccggg tgcagatgag
     5041 cggctgacgt cgcctcggtg ggacaacgcg cagtccacag ggccaccatg acggggtgga
     5101 tgccgttgcg cgcttcgcgg agctggtcgg gcatcccgat ccgccgctgg accgcgccgc
     5161 gctcgccctc gccgcgggcg ccgacgcgga gctcgatccc gcgcggtggc tggccgagct
     5221 cgaccggctc gcggacggcg tcacctcgct cgatgcactc gtgcatcggc tgttcgtgaa
     5281 ggaggcgttc gcgggcaacg tgcacgacta ctacgacccc cgcaattccc tgctcccgca
     5341 cgtcctcacc cgcaagctcg ggatcccgat caccctcgct atcgtcacga tcgaggtcgg
     5401 caggcgggcc ggcatcgcgc tggaaggcgt cgggatgccc gggcacttcc tggtccgggc
     5461 ggtcgggacg cacaccgtcc tcgacgtctt ccacggcggc atcgagctcg acgcagccga
     5521 ctgcgaggcg ctgttccgat cctcctcggg agccggcccc gacgtgtcgt tcggcccaca
     5581 cctgctggcc accgccccga cgcgctcgat cctgctgcgg atgctggaga acctgcgcct
     5641 cgtgtaccgg attcggcaac gccccgggga cctcgagtgg gtcgtgcgga tgcggctcgc
     5701 gctaccgggc gccgggctcg ccgaccttct cgagctggcc acggcactgg gggaccaggc
     5761 ccgctggctc gagggggccc ggctgctgga ggagcagctc ccgaccgctt ccgcggccgg
     5821 ggcggagcgg ctccgcactg cggcacgcac tctacgggcg cacctcaact gacctggagc
     5881 ggtcggatcc cccagctgcc cgtccacgtc tcccccggcg cgatggtgag cagccccgcg
     5941 ccggagttca gtgcgtcc
//
LOCUS       DASRSC010000371        13583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3760583,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000371 DASRSC010000000
VERSION     DASRSC010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 13583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13583
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3760583"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2174
                     /gene="ligA"
                     /locus_tag="VFE39_18015"
     CDS             <1..2174
                     /gene="ligA"
                     /locus_tag="VFE39_18015"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET6260537.1"
                     /translation="RVQKEVGEELHYLCELKIDGLAVNLLYEDGKLTRALTRGDGRSG
                     EDVTLNMRTLREVPEQLTGTAEFPVPALVEVRGEVYFRLEDFQALNASLVEAGKPPFA
                     NPRNTAAGSLRQKDPKVTASRHLRLICHGFGKREGFALERQSQGYDALRAWGLPVSDR
                     TVVLDGVDEVVAHVEYWGEHRHDIEHEIDGVVVKVDEVPLQRRLGSTSRAPRWAIAFK
                     YPPEEATTKLLDIQVNVGRTGRVTPFAQMEPVVVAGSTVSLATLHNADEVRRKGVLIG
                     DHVVIRKAGDVIPEVLGPVVDLRDGSEREFVMPTHCPECGTRLVRQKAGDVDQRCPNA
                     RSCPAQLRERLFHVAGRGAFDIEGLGYEAATALLVSGVVQDEGDVFTLTEEDLLRVEL
                     FRTKAGELTANGHKLLANLQAAKDRPLWRVLVGLSIRHVGPTAAQALAREFASMDAIE
                     AAAEAAARDTAAAGIAITSDGSLDDPDDSLGAAATSSALPTGDAATDAAAPDGAAGND
                     AERSGDEAVIDGEAADAAAAEGAALDGAGPEVAAPSAADGAAEAGAPELSEDERVAAA
                     QRAARVAARAQTKALAAALAPIAGVEGVGPTIAAAVRDWFTVDWHREVVQKWRAAGVR
                     MVDEVDESVPRTLDGLSIVVTGSMDSYSRDEAKEAITARGGRAAGSVSKKTAFVVAGD
                     APGSKYEKALEIGVPVLDEAGFRVLLERGPDAARDVATVYG"
     gene            2285..3004
                     /locus_tag="VFE39_18020"
     CDS             2285..3004
                     /locus_tag="VFE39_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HET6260538.1"
                     /translation="MLQAVLNGSRPADAHPALPVLARHLARDARDVAQLGITSVHVHP
                     RGPDQQETLDPIYVGDTVAAIRAQVPGMEIGVTTGRWIEPDPVKRIEAVLAWGHLGVG
                     KPDVCSVNVHEKGWVDVCSAAASVGIGVELGVWTSGDAVTLRNSGLPRGTTRVLAEST
                     VSDPETSVAEAVRILRALGTLPIPILLHGEEDGAWPVLEYAMRMGIDARIGFEDVLVT
                     PRGWLATGNDHLAELALEIRV"
     gene            3096..4169
                     /locus_tag="VFE39_18025"
     CDS             3096..4169
                     /locus_tag="VFE39_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010225296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin reductase"
                     /protein_id="HET6260539.1"
                     /translation="MGVLGEIGQRARVAATWFTQPLLPDDLLGTLNPLWSTREPHARV
                     VGLRRETADTTTLLLRTGTARPGHRAGQFVGIGTRLDGVWHWRTYSVTSCPADPLLAV
                     TVTAVPGGTVSGALAHRTPVGTLLRIGPPAGEFVLPDQVPEKLLFLTAGSGITPVMGM
                     LRDLVASRPEALGSAVLVHCDRTPSDLVFGPELRALSTTTGLTLVERHTAVEGRLTPD
                     NLTDVVPDWAARKAWACGPAGLLDALTEHWATAGDPAALHVELFTPPATIAPDATGGR
                     VSFTTSGIEADAGPGTPLMVAGEAAGALLPNGCRMGICHTCVGRLRSGAVRDLRTGDL
                     HDTPGEPVRTCVSGAAGDVEIEL"
     gene            4181..5362
                     /locus_tag="VFE39_18030"
     CDS             4181..5362
                     /locus_tag="VFE39_18030"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020644577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HET6260540.1"
                     /translation="MATQTFAPSMTPSAAAHLTDEQVETLGAELDALRAEVVGSLGEA
                     DARYIHRVIAVQRGLEVGGRGALIFSLFPPAWVVGTAALSVAKILDNMEIGHNVLHGQ
                     WDWMRDPKIHSTTWEWDHASPAASWKKSHNYEHHTFTNIRGKDRDLGYSIMRITPEQE
                     WKPTDLFQPLVNLGLSMIFEWGIAIYDIEIEKVKDGTKPWPQAKAELMGLWRKARRQL
                     AKDYVLFPLLSGPGFLSTVAANATANVVRNVWSHAVIFCGHFPDGVETFDFDEERLEG
                     ETKGQWYLRQMLGSANLSGSKLFHLMTGNLSHQIEHHLFPDVPSNRYAEIAPRVQEIC
                     RRYGLPYVTGSLPRQYGKVLRKIARLAFPGGGPAPAATPPVRLPDQARRPLAAPARLA
                     S"
     gene            complement(5472..6728)
                     /locus_tag="VFE39_18035"
     CDS             complement(5472..6728)
                     /locus_tag="VFE39_18035"
                     /inference="COORDINATES: protein motif:HMM:NF024945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET6260541.1"
                     /translation="MDRPWSRVPSWVGAALRPELAGTTDEIIAAVRAEVPDYARPLTG
                     RFGMRITEGVSVALGQFVDLLGRDEPLGDSRVYRALGQLEHREGRTLAALQLAYQVGT
                     RTMWRRLGGSTTARQLPPEVIFSLAEALFSYVEQITAASVAGWAAEESSRASSLQARR
                     HALVEVLARHPPAAPAEVDRVAAAAGWVLPARLAALVVEDAVAVAARLPGAIAADLDP
                     VGVVIVTVTSIGAARTNHVAGSGGARIDPASDNTAWLDRVRTRLGGRPAVLGPVVDPA
                     QAHRSVARAQAAWPLHVAGLLHPGSDETLARADDHLLALLLAAEPDLAADLYERAVAP
                     LRTLPAGAAARGEETLRAWLDAHGDITATAAALHVHPQTVRYRLAGLRDTFGGALDDP
                     AARLELAVALRMSVDSCCGTDHTTES"
     gene            complement(6741..7397)
                     /locus_tag="VFE39_18040"
     CDS             complement(6741..7397)
                     /locus_tag="VFE39_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HET6260542.1"
                     /translation="MSFLLRVVLPDKPGSLGAVATALGNAGADILGVDVVERAHGHAI
                     DDLAVELPPGRPPDVLITAAESVPGVEVESVRPHSGKLETHRELELIEAITGEPARGL
                     QLLADGVPRIFRAGWALVAERDGSRSYRLAESSAAPETRAGDLPWLPLRRAIVVDPGV
                     HWVPEPWSALDTELAAAPFGPGPRALVVGRPGGPAFRPSELARLTHLAGIVTTVLGSQ
                     "
     gene            7766..8095
                     /gene="gatC"
                     /locus_tag="VFE39_18045"
     CDS             7766..8095
                     /gene="gatC"
                     /locus_tag="VFE39_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET6260543.1"
                     /translation="MSADPATSGSSTGITRDEVAHLARLSRLAVTDAELDLFAGQLEV
                     ILSAVSRVGEVAADDIPPTSHAVPLQNVFRPDELRPGLTQEQALACAPAAEGGRFRVP
                     QILGEEA"
     gene            8092..9612
                     /gene="gatA"
                     /locus_tag="VFE39_18050"
     CDS             8092..9612
                     /gene="gatA"
                     /locus_tag="VFE39_18050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET6260544.1"
                     /translation="MTAELTRLTAAELAKKIHSREVSAVEVAQAHLDRIAAVDGEVHA
                     FLHVATGAALASAGMVDESLAAGTAPASPLAGVPLALKDVFTTLDMPTTAGSKILEGW
                     RPPYDATVSSRLRAAGITILGKTNMDEFAMGSSTEYSAYGTTRNPWDTTRVPGGSGGG
                     SAAALAAFEAPLAIGTDTGGSIRQPAAFTGTVGVKPTYGGVSRFGLIACASSLDQGGP
                     CARTVLDAALLHEVIAGHDPLDSTSIDERVPAVVEAARLGASGDLSGLRIGVVREMGG
                     EGYQSGVRASFEEALRQLEKLGAEAVEVSCPHFEYGLAAYYLILPSEVSSNLARFDAM
                     RYGLRVDKGASAEEVMAATREAGFGPEVKRRIMLGTYALSSGYYDAYYGQAQKVRTLI
                     ARDFAAAFAQADVLVSPTTPTTAFPIGDKVDDPLAMYLNDLATIPTNLAGTAALSVPS
                     GLADGLPVGLQVMAPALGEAVMYRVGAAYEAARNAEDGGPLIHRAPSLHTQVGDAA"
     gene            9609..11138
                     /gene="gatB"
                     /locus_tag="VFE39_18055"
     CDS             9609..11138
                     /gene="gatB"
                     /locus_tag="VFE39_18055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005444061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET6260545.1"
                     /translation="MSELASESSTRRAATLVDFDEVVERFDPMLGLEVHVELNTNTKM
                     FCGCPTAFGAEPNTQVCPTCLGLPGSLPVVNRTAVESAMRIGLALNCDIAPWGRFARK
                     NYFYPDMPKNFQTSQYDEPIAVNGHLDVLLDDGTTVRVEIERAHMEEDTGKSLHVGGA
                     TGRIHGAEYSLLDYNRAGVPLIEIVTKMIPGTRERAPEVARAYVTALRDLLRALGVSD
                     VRMDQGSMRCDVNLSLSPRDSGILGTRTETKNVNSLRSVERAVRYEMTRQAGVLLAGE
                     PIVQETRHFEEAGGTTRPGRRKETAEDYRYFPEPDLVPIAPPAEWVEELRGTLPELPW
                     ERRRRLQADWSLTDEELRDLVNAGALDLIEATVAAGAPAGEARSWWVAYLTQQANARG
                     VELPGLPITPEQVARVIELVASGSLNNKLARQVVDGVLADEGSPDDVVAARGLAVVSD
                     EGALVTAVDEALAAQPDIAEKIRGGKVQAAGAIVGAVMKATKGQADAKRVRELVLERC
                     T"
     gene            complement(11148..11771)
                     /locus_tag="VFE39_18060"
     CDS             complement(11148..11771)
                     /locus_tag="VFE39_18060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260546.1"
                     /translation="MTSPGEWGPWVDGGAWLVIDNTPVDWIYRNIDRVHSSWAAARAG
                     RFQFHFQVGHPLGVPDFAYAGEIALAVILADPTGELATLQEGTRDYPSALRRALVAAL
                     WEAGFAIGNAKKARARTDTAYISGCLFRVVGLCAHALHGAAGRWLITEKGAVQAAHDL
                     PNAPPEFALHAHRVLGHVGTTEPELDAAIADATELLAATTRACDAEG"
     gene            complement(12274..13503)
                     /locus_tag="VFE39_18065"
     CDS             complement(12274..13503)
                     /locus_tag="VFE39_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6260547.1"
                     /translation="MFNAPHAGVDPFAVTVRSVGEPHPAHAALRAVGPIVRADAPAGG
                     PAWIITDYALAREVLAHPRIVKDPAYAPAHWDPRIARLEPPVAAQPSLTTLDGPAHEQ
                     LRRAHTPLFTARRMHDYSGRITGIARELLTGIPAADGTVDLTADFTTRFPLAVICEVL
                     GVPLDRIDQAIAACRGMYGDDPEVEIAMAAFAELAAAGLRDGRDGLAAALRDAVPDEI
                     TETQLHYLLFTLIYAGQLTTDPALGFLLARVLDIDRAAAEGTTIDEFVRETLRLHSPA
                     PFTLWRFTSADVDLAGVRLPARSPVLVDIQGINTDPGRPIGPDLTFGAGPHYCTGAQL
                     AQLELSAVVEVLRTDFPDARLAVPFAELQQIDIGGIQGSRLTALPVTLTGPPSTGLGG
                     VPVVDAPTRGAAGSPPC"
BASE COUNT         1943 a         4739 c         5001 g         1900 t
ORIGIN      
        1 agcgggtgca gaaggaggtg ggggaggagc tgcactacct ctgtgagctg aagatcgacg
       61 gtctggcggt caacctgctc tacgaggacg gcaagctcac ccgcgcgctc acccggggcg
      121 acggccgctc gggcgaggac gtcacgctca acatgcgcac cctgcgtgag gtgcccgagc
      181 agctcaccgg cacggccgag ttcccggtgc ccgcgctggt cgaggtgcgc ggtgaggtct
      241 acttccggct ggaggacttc caggccctca acgcctcact ggtggaggcg ggcaagccgc
      301 cgttcgccaa cccgcgcaac accgcggccg ggtcgttgcg gcagaaggac ccgaaggtca
      361 ccgcatcgcg ccacctccgg ctgatctgcc acggcttcgg caagcgcgag gggttcgcgc
      421 tggagcggca gtcccagggc tacgacgcgc tgcgggcatg ggggttgccg gtctcggacc
      481 gcacggtggt cctcgacggc gtcgacgagg tcgtcgcgca cgtcgagtac tggggcgagc
      541 accgccacga catcgagcac gagatcgacg gcgtcgtcgt caaggtcgac gaggtgccgt
      601 tgcagcggcg gctcgggtcc acctcacggg cgccgcgctg ggccatcgcg ttcaagtacc
      661 cgccggagga ggccaccacg aagctcctcg acatccaggt caacgtgggc cgtacgggcc
      721 gggtcacgcc gttcgcgcag atggagccgg tcgtcgtcgc cgggtccacc gtctcgctgg
      781 ccaccctcca caacgccgac gaggtgcggc gcaagggcgt gctcatcggc gaccacgtcg
      841 tcatccgcaa ggcgggcgac gtcatccccg aggtgctcgg gcccgtcgtc gacctccgcg
      901 acggctccga gcgcgagttc gtcatgccga cgcactgccc cgagtgcggc acccgcctgg
      961 tccggcagaa ggcgggcgac gtcgaccagc gctgccccaa cgcacgctcc tgtcccgcgc
     1021 agctgcgtga gcggctgttc cacgtggcgg ggcggggcgc gttcgacatc gaggggctgg
     1081 gctacgaggc ggccacagcg ctgctcgtct ccggggtcgt gcaggacgag ggcgacgtct
     1141 tcaccctcac cgaggaggat ctgctgcgcg tcgagctgtt ccgcacgaag gcgggggagc
     1201 tgacggccaa cggccacaag ctgctggcca acctgcaggc ggcgaaggac cggccactgt
     1261 ggcgggtgct ggtcgggttg tcgatccggc acgtcgggcc cacggcagcg caggcgctcg
     1321 cccgcgagtt cgcctcgatg gatgcgatcg aggcggctgc ggaggccgcg gcccgagaca
     1381 ccgccgcagc ggggatcgcc atcacctccg acgggtcgct ggacgacccg gacgattccc
     1441 tcggtgcggc ggccacctca tcggctctcc ccacgggcga tgcggccacc gatgccgcgg
     1501 cccccgacgg ggccgccggc aacgacgcag agcgttcagg cgacgaagcg gtgatcgacg
     1561 gggaggcggc cgacgcagcg gcggccgagg gtgcagctct cgacggcgcc ggtccggagg
     1621 tcgcggcccc ctcggctgca gacggagcgg ccgaggcagg ggcgccggag ctctccgagg
     1681 acgagcgggt cgcagccgct cagcgggcgg cgcgggtggc ggccagggcg cagacgaagg
     1741 cactcgccgc ggcgctggcg cccatcgccg gggtggaggg cgtcggcccc accatcgctg
     1801 ccgcggtgcg cgactggttc acggtcgact ggcaccggga ggtcgtccag aagtggcgtg
     1861 cggcgggtgt ccgcatggtc gacgaggtgg acgagtccgt accgcgcacg ctcgacgggc
     1921 tctccatcgt cgtcaccggg tcgatggaca gttactcccg tgacgaggcc aaggaggcga
     1981 tcacggcccg cggcggccgg gccgcaggct cggtgtcgaa gaagacggcg ttcgtggtgg
     2041 ccggcgacgc tcctggctcg aagtacgaga aggcgctgga gatcggcgtg ccggtcctcg
     2101 acgaggcggg tttccgggtg ctcctggagc gcgggccgga cgcggcgagg gatgtggcga
     2161 ccgtctacgg atgacccccg atcgtgtcag ccacaggtcc gtatgatcga cgtgccgaga
     2221 gtctccggtg gtgggacata cggccatatg gtgactatta ctctcattgg tagggggtgt
     2281 cagggtgctg caggcggttc tgaacggatc gcggcccgcg gatgcgcatc ccgcgctccc
     2341 cgttctcgcc cggcatctcg cgcgcgatgc tcgcgacgtg gctcaactgg ggatcacgtc
     2401 ggtacacgtg cacccgcgtg ggcccgatca gcaggagacg ctcgacccga tatacgtcgg
     2461 cgacacggtt gcggcgatcc gcgctcaggt accggggatg gagatcggcg tcacgaccgg
     2521 tcgctggatc gaacccgacc ccgtcaagcg catcgaggcg gtgcttgcgt ggggccacct
     2581 cggggtcggc aagcccgacg tctgctcggt caacgtgcac gagaaaggct gggtcgacgt
     2641 ctgttccgcg gcggcctcgg tcgggatcgg cgtcgagctc ggagtctgga ccagcggcga
     2701 cgccgttacc ttgcgcaaca gcggcctgcc acggggcacc acgcgtgtgc tcgccgagtc
     2761 caccgtctcc gacccggaga cctcggtggc cgaggcggtg cggatcctcc gcgccctcgg
     2821 tacgctcccg atcccgatcc tcctgcacgg tgaggaggac ggcgcctggc cggtgctgga
     2881 gtacgccatg cggatgggta tcgacgcgcg catcgggttc gaggacgttc tggtgacccc
     2941 gaggggttgg ctcgccacgg gcaacgatca cctcgcggag ctcgcgctgg agatccgcgt
     3001 ctgatccacg cttttggcta tcgcaacaga aggaacggcc cggacgtggt cagcgacgtg
     3061 gccagctgtg cgaagtgcgc aataccgtcg agtacatggg agtgctgggc gaaatcggac
     3121 agcgggcacg ggtagcggcc acctggttca cgcagccgct gctgcccgat gacctgctcg
     3181 gcacgctgaa cccgctgtgg tccacccggg aaccccacgc ccgcgtggtg ggtctgcgcc
     3241 gggagaccgc cgacaccacc accctcctgc tgcgcaccgg gaccgcacgt ccggggcacc
     3301 gggcgggcca gttcgtcggc atcggcaccc ggctggacgg ggtgtggcac tggcggacct
     3361 actcagtgac gtcctgcccc gctgacccgc tgctcgccgt gaccgtgacg gccgtgccgg
     3421 gcggcacggt gtcgggggcg ctggcgcacc gcaccccggt ggggacgctg ctgcggatcg
     3481 gtcctccggc cggcgagttc gtgttgccgg accaggtgcc ggagaagctc ctcttcctca
     3541 ctgcgggcag cgggatcacc ccggtcatgg gcatgctccg cgacctggtc gcctcccggc
     3601 ccgaagcgct cggcagtgcg gtgctcgtgc actgcgaccg cacgccgtcc gacctcgtgt
     3661 tcgggcccga actgcgcgcg ctctccacca ccaccgggtt gacgctggtg gagcgacaca
     3721 ccgccgtcga gggcaggctc accccggaca acctcaccga cgtcgtcccc gactgggcgg
     3781 cccgaaaggc gtgggcctgc gggcccgcag gcctgctcga cgccctcacc gagcactggg
     3841 ccacggccgg cgacccggcc gcgctgcacg tcgagctgtt cacgcccccc gcgaccatcg
     3901 ctcccgatgc caccggcggc cgcgtgtcct tcaccaccag cgggatcgag gccgacgccg
     3961 gcccgggcac gccgctgatg gtggcgggcg aggcggcggg agcgctgctg cccaacggct
     4021 gccgcatggg catctgccac acctgcgtgg gccggctgcg ctccggcgcc gtgcgcgacc
     4081 tacgcaccgg cgacctgcac gacacccccg gcgaacccgt ccgcacctgt gtctccggtg
     4141 cggcgggcga cgtcgagatc gaactctagg aggcacctcc atggccacac agaccttcgc
     4201 accctccatg acgcccagcg ctgccgcgca cctcaccgac gagcaggtcg agaccctcgg
     4261 cgccgagctc gacgccctcc gcgccgaggt ggtcgggagc ctcggtgagg ccgacgcccg
     4321 ctacatccac cgggtcatcg cggtgcagcg gggcctcgag gtcggcggcc gcggcgcgct
     4381 gatcttctcc ctgttcccgc ccgcctgggt agtcggcacg gctgcgctgt cggtggccaa
     4441 gatcctcgac aacatggaga tcggccacaa cgtcctgcac ggccagtggg actggatgcg
     4501 cgacccgaag atccactcga cgacctggga gtgggaccac gcctcgcccg cggcgtcgtg
     4561 gaagaagtcg cacaactacg agcaccacac cttcaccaac atccgcggca aggatcgcga
     4621 cctcggctac tcgatcatgc ggatcactcc ggagcaggag tggaagccca ccgacttgtt
     4681 ccagccgctc gtgaacctcg gactctcgat gatcttcgag tggggtatcg cgatctacga
     4741 catcgaaatc gagaaggtca aggacggcac caagccgtgg ccgcaggcga aggcagagct
     4801 gatgggcctc tggcgcaagg cccggcgcca gctcgcgaag gactacgtgc tgttcccgct
     4861 gctgtccggc cccgggttcc tcagcacggt ggcggccaac gccaccgcca acgtcgtgcg
     4921 gaacgtctgg tcgcacgcgg taatcttctg cggccatttc ccggacggcg tcgagacctt
     4981 cgacttcgac gaggagcggc tcgagggtga gacgaagggc cagtggtacc tccggcagat
     5041 gctggggtcg gccaacctgt ccggcagcaa gctcttccac ctgatgaccg gcaacctgag
     5101 ccaccagatc gagcaccacc tgttccccga cgtccccagc aaccgctacg cggagatcgc
     5161 gccgagggtg caggagatct gcaggcgcta cggcctgccg tacgtcaccg ggtccctgcc
     5221 gcggcagtac ggcaaggtgc tgcgcaagat cgcccggctg gccttccccg gcggcggccc
     5281 cgccccggcg gccaccccgc ccgtccggct gcccgaccag gcccgccggc ccctggccgc
     5341 cccggcgcgc ctggcctcct gacgccgacg aaaatccgca gcgccttcgt acgggggtct
     5401 cggccgatag ggccgcccga acgcgccgtt cgtgcggggg aggggtgccc tgccggtccc
     5461 gggcggcggg gtcagctctc cgtggtgtgg tcggtaccgc agcagctgtc caccgacatg
     5521 cgcagggcca cggccagctc cagccgggcg gccgggtcgt ccagcgcccc accgaacgtg
     5581 tcccgcagcc cggccagccg gtaccgcacg gtctgcgggt gcacgtgcaa tgcggctgcg
     5641 gtagcggtga tgtcgccgtg cgcgtccaac caggcgcgca gcgtctcttc gccgcgggcg
     5701 gccgcgcctg cgggcagcgt gcgcagcggt gcgacggcgc gctcgtacag gtcggcggcg
     5761 aggtcgggct ccgccgcgag gagcagcgca agcaggtgat cgtcggcgcg ggccagcgtc
     5821 tcgtcggagc cggggtgcag caggcccgcg acgtgcagtg gccatgccgc ctgcgcgcgg
     5881 gcgaccgagc ggtgggcctg ggccgggtcg accaccgggc cgagcaccgc aggccgcccg
     5941 cccagcctgg tccggacccg gtcgagccag gcggtgttgt ccgatgccgg gtcgatgcgt
     6001 gccccgcccg atcctgcgac atggttggtg cgcgctgcgc cgatgctcgt gacggtcacg
     6061 atgacgacgc cgaccgggtc gaggtcggcg gcgatcgccc cgggcaaccg ggccgcgacc
     6121 gccacggcgt cctctacgac gagcgccgcc agccgggccg gcagcaccca gcctgctgcg
     6181 gcggccaccc ggtcgacctc ggcgggtgcc gccggcgggt gccgggcgag cacctccacc
     6241 agcgcgtgcc ggcgggcctg gagtgagctc gcgcggctgg actcctcggc agcccagccg
     6301 gccaccgatg cggcggtgat ctgctcgacg tagctgaaca gcgcttccgc caggctgaag
     6361 atcacctcgg gcggcagctg ccgggccgtc gtgctgccgc cgagccggcg ccacatcgtc
     6421 cgcgtgccga cctgatacgc caactgcagc gccgcgagtg tgcgcccctc acggtgctcg
     6481 agctggccga gggcccggta gaccctggag tcgccgaggg gctcgtcgcg accgagcagg
     6541 tcgacgaact gcccgagcgc caccgagacg ccctcggtga tccgcatccc gaaccggccg
     6601 gtcagcggcc gggcgtagtc cggcacctcg gcgcgcacgg cggcgatgat ctcgtcggtc
     6661 gtcccggcga gctccggccg aagcgccgcg ccgacccagc tgggcacgcg ggaccagggc
     6721 cggtccatgt cagcgtccca tcactggctg ccgagcaccg tcgtcacgat cccggcgagg
     6781 tgggtgagac gcgccagctc cgagggccgg aacgccggac cgcccggccg gccgaccacc
     6841 agtgcccgcg ggccgggtcc gaacggcgcc gccgcgagct cggtgtcgag ggcgctccac
     6901 ggttcgggca cccagtgcac cccggggtcg acgacgatgg cgcgtcgcag cgggagccac
     6961 ggcaggtcgc ccgcacgggt ctccggtgcc gccgagctct cggccagccg gtaggaccgc
     7021 gagccgtcgc gctcggcgac gagtgcccag ccggctcgga agatccgtgg cacgccgtcg
     7081 gcgagcaact gcaggcctcg cgccggttcg ccggtgatcg cctcgatcag ctcgagctcg
     7141 cgatgcgtct cgagcttccc ggaatgcggg cgcaccgact cgacctccac acccggcacg
     7201 gactcggccg cggtgatcag cacatccggt ggccgtcctg gcggcagctc gacggccagg
     7261 tcgtcgatgg cgtgcccgtg tgcccgctcg acgacatcga cgccgaggat gtctgcgccc
     7321 gcgttgccca gcgccgtggc gacggctccg agactgcccg gcttgtccgg cagcaccacc
     7381 cggagcagga acgacacact tcacgtcctc tcgcgaaaag cacccagccg cgatggacct
     7441 gcgccactcg ggcgggccga cgcggcactc cgagcaggat tgtgtcagga cgggccccct
     7501 ccggcgcaac atcttccgca ccggaggcgg cccgcggaat ccccactccg gacacggtgg
     7561 attcggctgg tggcggcggc cccggcacat gtcgcgcaga ccaccgcggc ccggcgaagg
     7621 cctggtcgtg ggacgactag acttgagtgg tcgacgtccc gcgccggtgc cgtttgcgtc
     7681 cgcccggttc gcggggcgcg gacgagagcg cacgccgaca ccatgacgcg cagcacctga
     7741 gcagtatgaa ccgccctgga gggacatgtc cgccgatccc gcgacgtcgg gtagcagcac
     7801 ggggatcacc cgggacgagg tcgcgcacct cgcccggctg tcccggctcg ccgtcaccga
     7861 cgcggagctc gacctcttcg ccggccagct cgaggtgatc ctctcagccg tgagccgggt
     7921 gggtgaggtc gccgccgacg acatcccgcc gacctcgcac gcggtgccgc tgcagaacgt
     7981 gttccggccc gacgagctac gccccggtct cacccaggag caggcgctcg cgtgtgcccc
     8041 cgccgccgag ggcggccgct tccgggtgcc ccagatcctg ggggaggagg catgaccgcc
     8101 gagctcaccc gcctcactgc cgcagagctc gccaagaaga tccactcgcg cgaggtgtcg
     8161 gctgtcgagg tcgcgcaggc ccacctcgac cggatcgcgg cggtcgacgg cgaggtgcac
     8221 gcgttcctgc acgtggcaac cggcgcggcg ctggcctcgg ccgggatggt cgacgagagc
     8281 ctcgctgccg gcaccgcgcc cgcgtcgccg ctggcgggcg tcccgctggc actcaaggac
     8341 gtgttcacca cgctcgacat gcccaccacg gccgggtcga agatcctcga gggctggcgc
     8401 ccgccctacg acgccacggt ctcgtcgcgg ctgcgcgccg cggggatcac gatcctgggc
     8461 aagaccaaca tggacgagtt cgcgatgggc tcctctaccg agtactcggc ctacggcacc
     8521 acccgcaacc cgtgggacac cactcgcgtc ccgggaggtt ccgggggcgg ctcggcagct
     8581 gcgctcgccg cgttcgaggc gccgctcgcg atcggcaccg acaccggcgg ctcgatccgt
     8641 cagcctgcgg ccttcaccgg cacggtgggg gtcaagccga cctacggtgg ggtttcccgg
     8701 ttcgggctca tcgcctgcgc ctcctcgctc gaccagggcg gcccgtgtgc ccgcacggtg
     8761 ctcgacgcgg cgctgctgca cgaggtgatc gccggacacg acccactcga ctccacctcg
     8821 atcgacgagc gtgtccccgc cgtcgtggag gcggcgcggc tcggggcgtc gggcgacctg
     8881 tccggcctgc gcatcggcgt ggtgcgcgag atgggcggcg agggctacca gtccggcgtc
     8941 cgggcctcgt tcgaggaggc gctgcggcag ctggagaagc tcggcgcgga ggccgtcgag
     9001 gtctcctgcc cgcatttcga gtacgggctc gcggcgtact acctgatcct gccgagcgag
     9061 gtctcctcga acctcgcccg cttcgacgca atgcgttacg ggctgcgcgt cgacaagggc
     9121 gcctcggcgg aggaggtcat ggccgccacc cgtgaggccg ggttcgggcc cgaggtgaag
     9181 cggcgcatca tgctgggcac ctatgcgctc tcgtcgggct actacgacgc ctactacgga
     9241 caggcgcaga aggtgcgcac gctcatcgcc cgcgacttcg ccgccgcgtt cgcccaggcc
     9301 gacgtgctgg tctcgccgac cacgcccacc acggcgttcc cgatcggtga caaggtcgac
     9361 gacccgctgg ccatgtacct caacgacctg gccacgatcc ccaccaacct ggcgggcacg
     9421 gccgcgctgt cggtgccgtc cgggctcgcg gacgggctcc ccgtcggcct gcaggtgatg
     9481 gcccccgcgc tcggcgaggc cgtcatgtac cgggtggggg cggcgtacga ggccgctcgc
     9541 aacgccgagg acggcggccc gctgatccac cgcgcgccgt ccctccacac acaggtggga
     9601 gatgccgcat gagcgagctc gcgagcgaat catcgacgcg gcgtgccgcc acgctggtcg
     9661 acttcgacga ggtcgtggag cgtttcgacc cgatgctcgg gctcgaggtg cacgtcgagc
     9721 tcaacacgaa cacgaagatg ttctgcggtt gccccaccgc gttcggggcc gagccgaaca
     9781 cgcaggtgtg tcccacgtgc ctgggcctgc cggggtcgct gccggtggtc aaccggacgg
     9841 ccgtggagtc ggcaatgcgg atcgggctcg cgctgaactg cgacatcgca ccctggggcc
     9901 ggttcgcgcg gaagaactac ttctaccccg acatgccgaa gaacttccag acctcgcagt
     9961 acgacgagcc gatcgccgtc aacgggcacc tggacgtgct gctggacgat gggacgacgg
    10021 tccgtgtcga gatcgagcgc gcgcacatgg aggaggacac cggcaagtcg ctccacgtcg
    10081 gcggcgcgac gggccggatc cacggtgccg agtactcgct gctggactac aaccgtgcgg
    10141 gcgtcccgct gatcgagatc gtcacgaaga tgatcccggg aacgcgggag cgggcacccg
    10201 aggtggcgcg tgcgtacgtc accgcgctgc gcgacctgct ccgggcgctc ggcgtctccg
    10261 acgtccgcat ggaccagggg tcgatgcgct gtgacgtgaa cctgtcgctg tccccgcgcg
    10321 actccgggat cctcggtacc cgcaccgaga cgaagaacgt caactcgctg cggtcggtgg
    10381 aacgcgccgt ccggtacgag atgacgcggc aggcaggcgt gctgctggcg ggtgagccga
    10441 ttgtgcagga gacgcggcac ttcgaggagg ccggcggcac cacccggccg ggccgccgca
    10501 aggagaccgc ggaggactac cggtacttcc cggagcccga cctggtgccg atcgcgccac
    10561 ccgccgagtg ggtcgaggag ctgcgcggca cgctgccgga gctgccgtgg gagcggcgtc
    10621 ggcggctgca ggccgactgg tcgctcaccg atgaggagct tcgcgacctc gtcaacgccg
    10681 gcgccctgga cctcatcgag gccacggtcg cggcaggtgc tcccgcgggc gaggcccggt
    10741 cgtggtgggt ggcctacctg acccagcagg cgaacgcgcg cggcgtcgag ctgcctgggc
    10801 tgccgatcac gccggagcag gtggcccggg tgatcgagct cgtggcgtcc gggtccctga
    10861 acaacaagct cgcccgccag gtcgtcgatg gcgtgctggc cgacgagggc tcgcccgacg
    10921 acgtcgtcgc ggcccgggga ctcgcggtcg tctccgacga gggcgcgctg gtgacggccg
    10981 tcgacgaggc gctggccgcg cagccggaca tcgcggagaa gatccgcggc ggcaaggtgc
    11041 aggccgcggg cgccatcgtc ggggcggtca tgaaggcgac gaagggccag gcagacgcca
    11101 agcgagtccg ggagctggtg ctggagcgct gcacctgacg gcctgtgtca accctccgcg
    11161 tcacacgctc tcgtggtggc cgcaagaagt tcggtcgcgt cggcgatggc ggcatcgagc
    11221 tcgggttcgg tggtgccgac gtgaccgagt acgcggtgtg cgtggagcgc gaactcgggc
    11281 ggtgcgttcg gcagatcgtg ggcagcctga acggcgccct tctccgtgat gagccagcgt
    11341 ccggcggctc cgtgcagggc gtgggcgcag agtccgacga cgcggaacag gcaaccgctg
    11401 atgtaggcgg tgtcggtgcg tgcgcgggcc ttcttggcgt tcccgatggc aaagccggct
    11461 tcccagagtg cggcgaccag tgcgcgccgc aaggcgctgg ggtagtcgcg ggtgccctcc
    11521 tgcagcgtgg ccagttcgcc ggtgggatcg gcgaggatca cggccagggc gatctcgccg
    11581 gcataggcga agtcgggaac gccgagaggg tgaccgacct ggaagtggaa ctggaagcgg
    11641 ccagctcggg ctgcggccca tgaggagtgg acgcggtcga tgttgcggta gatccagtcg
    11701 acgggcgtgt tgtcgatgac gagccatgcg ccgccgtcga cccagggccc ccactccccg
    11761 ggcgaggtca ctcgagcatc aggtccggcg gtgtcgcgcg caatctcgcc gagttgctgc
    11821 agatccaagg gtggcctgta gtacaggcca agatcgatgt cggactcggc ggtatgtgtg
    11881 cctcgtgcac gactcccgcc caaggtgacc ccgacaacgc cgggcacggt catgaggcgt
    11941 tgagcaagtg ctcgtagctg ttcgtggtcg agcatgcctt catcgttccg cacacgcaca
    12001 cccattggcc gatcccacca gggagtgcgc accggcggtg gcatcgccgg gcggggactc
    12061 cggtagggct gtcgagggtt cggtcggaga gtgcggcatg tcgtcctccg gtgcgggtcg
    12121 aagacggacc cgcgcttggg tctgggtcgc ggctccggca tcgctcactg ttcggcgctc
    12181 tacccggcgc tcagcccttg tcgacggtcc gcgcgaccag gtgaccacca ggcccaggcc
    12241 ccgcgtgtct cagggcggac gtcgggtgtc ggctcaacag ggcggtgacc cagcggcacc
    12301 tcgggtgggt gcatcgacga cggggacgcc gccgagacct gttgagggtg gaccagtgag
    12361 cgtgactggg agggcggtca agcggcttcc ttggatgccg ccgatgtcga tctgttgaag
    12421 ttcggcgaac ggcaccgcaa ggcgggcgtc ggggaagtcg gtcctgagca cctctacaac
    12481 cgcgctcagc tcgagttggg cgagttgggc gccggtgcag tagtgcgggc cggcgccgaa
    12541 ggtgaggtcc ggcccgatcg gccggccggg gtcggtgttg atgccctgga tgtcgacgag
    12601 caccggggag cgagcgggga gccgcacccc ggcgagatcg acgtccgcgg aggtgaaccg
    12661 ccagagggtg aagggtgccg gcgagtgcag gcgcagcgtc tcccggacga actcgtcgat
    12721 ggtggtcccc tccgctgccg cgcggtcgat atcgaggacg cgcgcgagca ggaatcccaa
    12781 cgccgggtcg gtggtgagct gtccggcata gatcagcgtg aagagcagat agtggagctg
    12841 tgtctcggtg atctcgtcgg ggaccgcatc gcgcagcgcg gcggcgaggc cgtcccggcc
    12901 gtcgcggagc ccggcggcgg ccagctccgc gaaggcggcc atggcgatct ctacctcggg
    12961 gtcgtcgccg tacattcccc gacaagcggc gatggcctgg tcgatcctgt cgagcggtac
    13021 gccgagcacc tcgcagatga cggcgagggg gaacctcgtg gtgaagtccg ccgtcaggtc
    13081 gaccgttccg tcagcggcgg ggattccggt gagcagctcg cgggcgatcc ccgtgatccg
    13141 ccctgagtag tcgtgcatcc gccgggcggt gaacagcggg gtgtgcgcgc ggcgcagttg
    13201 ctcgtgcgcc gggccgtcga gggtggtgag cgacggctgc gcggcgacgg gtggttcgag
    13261 acgtgcgatc cgcggatccc agtgcgccgg cgcgtacgcg gggtccttca cgatgcgcgg
    13321 gtgagcgagg acctcccggg ccagcgcata gtcggtgatg atccatgctg ggccgccggc
    13381 cggggcgtca gctcggacga tcgggcccac ggcgcgcagg gcggcgtggg cgggatgggg
    13441 ttcgccgacg ctgcggacgg tgacggcgaa cgggtcgacg ccggcgtgag gcgcgttgaa
    13501 catcaaggct ccttggaagc tgcctggatc gtggtgggta gggctgttgt cgacggctgg
    13561 cccggatggt tccgacggcg tgg
//
LOCUS       DASRSC010000372         7848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_2127044,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000372 DASRSC010000000
VERSION     DASRSC010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2127044"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1998)
                     /gene="secA"
                     /locus_tag="VFE39_18070"
     CDS             complement(<1..1998)
                     /gene="secA"
                     /locus_tag="VFE39_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET6260548.1"
                     /translation="MRSTVPLLNRLLRAGETKLVKRLARYADDIDKLEPEVQALTDAQ
                     LRAKTEEFKERYQAGESLDDLMFEAFAVVREGAVRTIGQRAFPVQLMGGAALHLGNIA
                     EMKTGEGKTLTSVFPAYLNAISGDGVHVVTTNDYLAKRDSENMGRIHRFLGLTVGVIL
                     SEMPPVVRRGQYAADITYGTNNEFGFDYLRDNMAWKAADMVQRGHNFAIVDEADSILI
                     DEARTPLIISGPAEQSARWYQEFARMSPLLKCDLHYEVDERKRTVGVTEEGVSLVEYQ
                     LGIDNLYEAANTPLVGYLNNALKAKELFKKDKDYIVRDGQVLIVDEFTGRVLAGRRYN
                     EGMHQAIEAKEGVEVQAENQTLATITLQNYFRLYETLSGMTGTAQTEAAELHQIYKLG
                     VVPIPTNRPMIRKDQPDLIYKTEEAKFAAVAADIAEKYRAGQPVLVGTTSVEKSEYLA
                     KLLVGLQVPHEVLNAKHHEREAAIVAQAGHAGAVTVATNMAGRGTDIVLGGNPEFLAD
                     LELRGRGLDPVERREEYEAAWDDTVAAMKAQVAAEAEEVKEAGGLYVLGTERHESRRI
                     DNQLRGRSGRQGDQGESRFYLSLGDELMRRFNGQLVESIMNRFNLPDDVPIEAGMVTK
                     AIRSAQTQVEQQNFEIRKNVLKYDEVLNKQRTVIYDERRRVL"
     gene            complement(2163..2795)
                     /gene="raiA"
                     /locus_tag="VFE39_18075"
     CDS             complement(2163..2795)
                     /gene="raiA"
                     /locus_tag="VFE39_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET6260549.1"
                     /translation="MEIVVTGRNVEVPEHFRVHVAEKVARLERYDHKIMGMEVELFHE
                     PNRRQLKNCQRVEITGRGCGPAARAEASAQDFYAALDAAVAKLDARLRRSHDRRRVTG
                     KRARVLAGRGAATAVLDELRQVDVSPDDVDIPGPRGHDEYLPTPEEPVPGRIVRSKVH
                     TADPMSVDDALSRMELVGHDFYLFADADSGEPCVVYRRKGYDYGMIRLAR"
     gene            complement(3011..3736)
                     /locus_tag="VFE39_18080"
     CDS             complement(3011..3736)
                     /locus_tag="VFE39_18080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015787378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HET6260550.1"
                     /translation="MRARELGAALVDLVLPGDCAGCGDPVHPWCPRCLVRLGEPCWPA
                     VPGVPGVLAVGRYTGPLRAALLRYKERGRRDLAAPLAALLAGPLDEVMQPPPAVTWLL
                     PAPSRPAAARARGGDHVVRLCRALAAGRPDVRVARPLRLMRRARDSVGLDAVQRAQNL
                     AGRLCVRPAALPPEGAAVLLVDDVVTTGATLRACRSALARAGVGVDVALVLCDATRRE
                     TRREEAPWRPGNADPRRERGVRS"
     gene            complement(4745..6481)
                     /locus_tag="VFE39_18085"
     CDS             complement(4745..6481)
                     /locus_tag="VFE39_18085"
                     /inference="COORDINATES: protein motif:HMM:NF022113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HET6260551.1"
                     /translation="MIPRRFGSALALVLALAGAAGCANVPESSDVQVLRQISEGEGGA
                     LPPGPVEGSNPLDLVRDFVFASGSSADEHGSARRFLAPDVAEWDDAASLTVLDGQFDT
                     VPAPGAQDASTGVTTIRIRGTAVGRMSSSGSFEPDQATFQQDVTVVRRDGQWRISSVP
                     AGVVVPLSIFRDNYRQVRTWFVDPVRRLVVADLRYVPSVPAKAQTSRALELLLAGPSP
                     ALLGAAVSQLPPGAQLRSNAAVGPDGALIVDLTGVGDLDEPARQLLAAQVVLTLAEVN
                     VAKVRLLVDGEPLLNDRPELTREDVAALSAEVQPGADVPGLVVAGGRVRQLTGPEPGA
                     PLPGPVGNGAYDVESAASTGDGQRLAAVVRIGARRTLLVGGGPDGGVSPVSLNAATMT
                     RPSWTPTGGEVWTVLDGAVVARVLVDSAGPARTAQVNADGLAALGPIRDLRLSRDGMR
                     VVAVVGGALYTAAVARSIDGEVAIRNIHRLRPDDLGEVVAADWRSAESIVAITRGTQM
                     LVGQVSVDGLVVQQVLGNNLTPPLTAIAAASNRPLLVTDQTGVWSFAGGDQAAWRQVL
                     GGAPDGAPLYPG"
     gene            complement(6478..>7848)
                     /gene="mtrB"
                     /locus_tag="VFE39_18090"
     CDS             complement(6478..>7848)
                     /gene="mtrB"
                     /locus_tag="VFE39_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MtrAB system histidine kinase MtrB"
                     /protein_id="HET6260552.1"
                     /translation="SDEPSAAAAGEFRAVLAASRGAGAVAAGPVEDVPADLRDAVAAG
                     TLSSKYVTLTEQGVDIPTLIVGQPVRTANGELEFYLLFPLTAEQRTLGWVQSTLIVGG
                     LILLLLLAGIAGLVTRQVVRPVRQAAEIAERFADGHLDERMPVQGEDDVARLGESYNE
                     MASSIQSQIRQLEEFGALQRRFTSDVSHELRTPLTTVRMAADVLYASREQLLPALRRS
                     SELLVAELDRFETLLADLLEISRLDAGVAELGAERVDMHGVVARAVEAVRGIADETGT
                     PLELELPTGVYAEVDPRRVERIVRNLVANAIDHGEGRPVNVVLAHDERVVAVLVRDHG
                     VGLRPGEAGLVFNRFWRAEESRARRSGGSGLGLSIAIEDARLHGGWLQAWGAVGQGAA
                     FRLTLPRTLGDAVESSPLPLGPEPGAVTGGGEASVPTPPRGRPVTVVVPSWSEGDDPH
                     EREDIP"
BASE COUNT         1120 a         2852 c         2750 g         1126 t
ORIGIN      
        1 gaggacccgg cgccgctcgt cgtagatgac ggtgcgctgc ttgttcagca cctcgtcgta
       61 cttgagcacg ttcttgcgga tctcgaagtt ctgctgctcg acctgcgtct gggcactgcg
      121 gatggccttg gtgaccatgc cggcctcgat cggcacgtcg tccggcaggt tgaaccggtt
      181 catgatcgac tcgacgagct ggccgttgaa ccggcgcatc agttcgtcgc cgagggagag
      241 atagaagcgc gactcaccct ggtcgccctg acggccggag cggccgcgta gctggttgtc
      301 gatgcgccgc gactcgtggc gctcggtgcc cagcacgtac aggccgcctg cctccttgac
      361 ctcctcggcc tcggcagcca cctgcgcctt catcgccgcg acggtgtcgt cccatgcggc
      421 ctcgtactcc tcgcgccgct cgaccgggtc gagcccgcgt ccgcgcagct ccaggtcggc
      481 gaggaactcg gggttgccgc ccagcacgat gtcggtgccg cggcctgcca tgttcgtggc
      541 cacggtgacc gcgccggcgt gcccggcctg ggcgacgatc gccgcctccc gctcgtgatg
      601 cttcgcgttg aggacctcgt gcggcacctg gagccccaca agcagcttcg ccaggtactc
      661 cgacttctcg acgctcgtcg tgccgacgag aacgggctgg ccggcacggt acttctcggc
      721 gatgtccgcg gcgaccgcgg cgaacttcgc ctcctccgtc ttgtagatca ggtccggctg
      781 gtccttgcgg atcatcggcc ggttggtcgg gatcggcacc accccgagct tgtagatctg
      841 gtgcagctcg gcggcctccg tctgggccgt gccggtcatc cccgacagcg tctcgtagag
      901 ccggaagtag ttctgcaggg tgatggtggc cagcgtctgg ttctccgcct ggacctccac
      961 cccctccttg gcctcgatgg cctggtgcat gccctcgttg tagcggcgcc ccgccagcac
     1021 gcggccggtg aactcgtcga cgatgagcac ctgcccgtcg cggacgatgt agtccttgtc
     1081 cttcttgaac agctccttgg ccttgagagc gttgttcagg tagcccacca gcggggtgtt
     1141 cgcggcctcg tagaggttgt cgatgccgag ctggtactcc accagcgaca cgccctcctc
     1201 ggtgacgccg acggtgcgct tgcgctcgtc cacctcgtag tgcaggtcgc acttgagcag
     1261 cggcgacatg cgggcgaact cctggtacca acgggcgctc tgctcggccg gccccgagat
     1321 gatcagcggg gtgcgggcct cgtcgatgag gatggagtcg gcctcgtcga cgatggcgaa
     1381 gttgtgcccg cgctgcacca tgtcggccgc tttccaggcc atgttgtcgc gcaggtagtc
     1441 gaagccgaac tcgttgttgg tgccgtaggt gatgtcggcg gcgtactggc cgcggcggac
     1501 cacgggcggc atctccgaca ggatcacccc gacggtgagg ccgaggaacc ggtggatccg
     1561 gcccatgttc tcggagtcgc gcttggccag gtagtcgttg gtggtgacga cgtgcacgcc
     1621 gtcgccggag atcgcattga gataggcggg gaacacggag gtgagcgtct tgccctcacc
     1681 ggtcttcatc tccgcgatgt tgccgaggtg gagcgccgcc ccgcccatga gctgcaccgg
     1741 gaaggcgcgc tggccgatcg tgcgcaccgc gccctcgcgg accacggcga acgcctcgaa
     1801 catgaggtcg tcgagcgact cgcccgcctg gtagcgctcc ttgaactcct cggtcttcgc
     1861 gcgtagctgg gcgtcggtca gcgcctgcac ctctggctcg agcttgtcga tgtcgtcggc
     1921 gtagcgcgcc agccgcttca ccagcttcgt ctcaccggca cggagcagac ggttcaagag
     1981 cggcacggtc gacctcattc accttcgcgg acaagtacgc gggttcttgt gtagcagccc
     2041 gccatgccac ggcgaactac ctgtccaggg tagcgaagct acggacatca ccctgcacaa
     2101 cgccggaggg gcggcacacg ttccggtcgg aacgcacgcc gcccctctcg ggtggtgtgc
     2161 agttaccgcg cgaggcggat catcccgtag tcgtaaccct tgcggcggta gacgacgcac
     2221 ggctcgccgc tgtcggcgtc ggcgaacagg tagaagtcgt ggccgacgag ctccatccgg
     2281 gacagggcgt cgtccacgct catcgggtcg gcggtgtgga ccttgctgcg cacgatgcgc
     2341 ccgggtaccg gctcctcggg ggtgggtagg tactcgtcgt gcccccgggg accggggatg
     2401 tcgacgtcgt cgggtgacac atcgacctgt cgcagctcgt cgaggacggc agtggcggcg
     2461 ccgcgcccgg ccagcaccct ggcgcgcttg ccggtgaccc tgcggcggtc gtgtgaacgg
     2521 cgcagccgcg cgtcgagctt cgcaaccgcc gcgtcgagcg cggcgtaaaa gtcctgagca
     2581 ctggcctcgg ctctggcggc cgggccacat cccctgccag tgatctcaac ccgctggcag
     2641 ttcttgagct gacggcggtt cggctcgtgg aagagctcga cctccatgcc catgatcttg
     2701 tggtcgtagc gttccagacg agcgaccttc tcggcgacat ggacccggaa atgctccggg
     2761 acctcgacgt tacggccggt gacaacgatc tccacgcaga cttccctcgc tctcagcgtc
     2821 gggttggcgt tgcagactgc accggggatt cccacccccg ggcgcacgat cggtggatcc
     2881 tcctgaatag gggcctcggc ggatgcggag cacggtaacg cgcatcacgg ttcggcaaca
     2941 gccctgagcg gtaagtggcg cggacctcgg tggatgcggt gaacaacatt cccaggacgc
     3001 ctcctggccg ctacgatcgc acgccccgtt cacgtcgtgg atcagcgttc ccgggccgcc
     3061 agggggcctc ctcccgcctc gtctcccgcc tcgtcgcgtc gcagaggacg agcgcgacgt
     3121 cgaccccgac accggcccgg gccagcgcgg agcggcaggc acgcagggtg gcgccggtgg
     3181 tgaccacgtc gtcgaccagg agcacggcgg ccccctccgg cggtagggcg gcaggacgta
     3241 cgcagaggcg tccggcgagg ttctgtgcgc gctgcacggc atcgagaccc accgagtcgc
     3301 gcgcccggcg catgagccgc agcggacggg caacccgcac gtcgggtcgg cccgcggcca
     3361 gcgcacggca gagccggacc acatggtcgc caccacgggc ccgcgctgct gcgggtcgcg
     3421 acggtgccgg cagcagccac gtgacggccg ggggcggctg catgacctcg tcgagcggcc
     3481 cggcaagcag cgctgcgagg ggcgccgcaa gatcgcgccg accgcgctcc ttgtagcgca
     3541 gcagcgcagc gcgcagcggg ccggtgtacc gccccaccgc cagcacgccg ggcacgcccg
     3601 gaacggcggg ccagcacggc tcccccagcc gcaccaggca ccgcggacac cacgggtgca
     3661 ccgggtcgcc acacccggcg cagtcgccgg gcagcacgag gtccaccagc gccgccccca
     3721 gctcccgagc cctcatgccc tcagcatgcg ctccggcacc acagcattgc cccgccgaac
     3781 ccccagttgt ccacacccca cccccccacc caccaccccc gcgagtcgcc cagaatccgc
     3841 ccgcgagtcg ccccctccta ccgcgcgagt cgcccagaat ccgcccgcga gtcgccccct
     3901 gcgcagctcg cgagtcgcca tctgcatgtc ggtgtccgcg tcgcacgtct gatccggctg
     3961 cacggggacc tcacgggatc agcgagtggg atcccgcagt ggtacggacc gctgggggtg
     4021 cggcccgaat cgtcgatcat gctggccgat cggacggccg gcgacatgat caacgcacgg
     4081 gccccggatc ggcacggacg acgggtcggt cccgaaagcg gatcgagctg ggccgatcgg
     4141 atcgacggtt gcgcgatcgc cggacggggc gcgtcggtaa gaaaccgctc tcgcccgcgt
     4201 ctggaacgct ggtcatgccg ggtggagaga tcagctgccg gccaaaggac ggggccgcgg
     4261 aacggcacag gccgccggtc tctcggcatc gatcatcgcg cgccgacgcg ctcgggcagc
     4321 gacatgatca cgcccggacg tggagcggca cccaccgcag tctccggttc gaaacgctga
     4381 tcatgctcgg cccgtgccgg gtggcatcct ccgccggcag acagtgcggt cagggacggg
     4441 accagatcgg tgcggacccg gtcgagcgcg ccgctgcgga tgatcatgcc cgcgggcatc
     4501 gggttggctg cccgcacgtc atcgcgtacc gacccacgag ccggtccaga atcgactcac
     4561 acgaaggttc ctggtccact cgcgcgtcgc ttcgggtcga actcgcgccc cgatatcggg
     4621 cgagaccccg gcgggaacgg gcgactcacg ggcgggaagc gggcgactcg cgggcgggaa
     4681 gcgggcgact cgcgagcggg aacagggcga ctcgcgggcg ggaacggggc gactcgcggg
     4741 tgggtcagcc ggggtagagg ggggccccat cgggggctcc gcccaggacc tgacgccagg
     4801 cagcctggtc accgccggcg aagctccaca ccccggtctg atcggtgacg agcagcgggc
     4861 ggttggaggc ggccgcgatt gccgtgagcg gaggggtcag gttgttcccg agcacctgct
     4921 gcacaacgag gccgtcgacc gagacctggc cgacgagcat ctgcgtaccg cgcgtgatgg
     4981 ccacgatcga ctcggcggag cgccagtcgg ccgcgacgac ctcgccgagg tcgtctggcc
     5041 gcagccggtg gatgttgcgg atggcgacct cgccgtcgat gctgcgggcc acagcggcgg
     5101 tatagagcgc tccgccgacg accgccacca cacgcatacc gtcgcgggag agccgcaggt
     5161 cgcggatcgg gccgagcgcg gccagcccgt ccgcgttgac ctgggcggtg cgcgcaggcc
     5221 cggcgctgtc gaccaggacc ctggcgacga ccgccccgtc caggacggtc cagacctcgc
     5281 cgccggtcgg ggtccaggac ggccgggtca tcgtcgcggc gttgagcgac acgggtgaga
     5341 ccccgccgtc gggcccgccg ccgaccagca gcgtccgcct cgcgccgatc cggacgacgg
     5401 ccgcgagccg ttggccgtcg cccgtggacg cagcggactc cacgtcgtag gcaccgttgc
     5461 ccacgggacc ggggagcggg gcgcccggct cggggccggt cagctgccgg acccggccgc
     5521 ccgcgaccac gaggccgggc acgtcggcac cgggctgcac ctcggcggac agcgccgcga
     5581 catcctcgcg ggtcagctcg ggccggtcgt tcagcagcgg ttcgccgtcc accaacagcc
     5641 gcaccttcgc cacgttgacc tcggccagcg tcagcaccac ctgtgcggca agcagctgcc
     5701 gcgccggctc gtcgaggtcg ccgactccgg tgaggtcgac gatcagcgcg ccgtccggtc
     5761 cgacggccgc attcgaccgc agctgggcac ccggcgggag ctgggagacg gccgcgccca
     5821 gcaatgccgg ggacgggccg gcgagcagga gctccagtgc gcgggaggtc tgcgccttgg
     5881 ccggcacgct gggcacgtag cgcaggtcgg ccaccacgag cctgcggacg gggtcgacga
     5941 accaggtacg cacctgtcgg tagttgtccc ggaagatcga cagcggcacg acgacaccgg
     6001 ccggcacgct cgagatccgc cactgcccgt cgcgacgcac gaccgtgacg tcctgctgga
     6061 acgtggcctg gtccggctcg aacgacccgg acgacgacat cctgccgact gccgtgccac
     6121 ggatccggat cgtcgtgacg cccgtgctcg cgtcctgggc cccgggggcg ggcacggtgt
     6181 cgaactgccc gtcgaggacc gtgaggctcg cggcgtcgtc ccactccgcg acgtcggggg
     6241 cgaggaagcg gcgtgcggag ccgtgctcgt cggcgctgct gcccgaggcg aagacgaagt
     6301 cacggaccag gtcgagcgga ttgctgccct cgacgggacc gggcggcagc gcgccgccct
     6361 cgccttcgct gatctggcgc agcacctgca cgtccgagct ctccggaacg ttcgcacagc
     6421 ccgccgcccc ggcgagcgcg aggaccaggg cgagcgctga accgaaccgg cggggaatca
     6481 cgggatgtcc tcgcgttcgt gggggtcgtc gccctccgac caggacggca cgaccaccgt
     6541 gacgggccgc ccgcgcggcg gcgtgggtac cgaagcctcg ccaccgcccg ttacggctcc
     6601 gggctcgggc ccgagcggca gcgggctgga ctcgaccgcg tcaccgaggg ttcgcggcag
     6661 tgtgagccgg aacgccgcgc cctggccgac cgcaccccac gcctgcagcc aaccgccgtg
     6721 cagccgcgcg tcctcgatgg cgatggacag gccgagtccg ctgccgccgc tgcgccgcgc
     6781 ccgcgactcc tcggcccgcc agaaccggtt gaacaccagc cccgcctccc ccggacgcag
     6841 cccgacgccg tggtcccgga ccagcacggc gacgacgcgc tcgtcgtggg ccagcacgac
     6901 gttgaccggc cgtccctcgc cgtggtcgat cgcgttggcc accaggttgc gcacgatccg
     6961 ctccacccga cgcgggtcga cctcggcata cacacccgtg ggaagctcca gctcgagcgg
     7021 tgtgccggtc tcgtcggcga tcccgcgcac ggcctcgacc gcccgcgcca ccaccccgtg
     7081 catgtccacc cgttcggccc ccagctcggc cacgcccgcg tcgagcctgc tgatctccag
     7141 gaggtcggcg agcagcgtct cgaaccggtc gagctccgcc acgagtaact cggagctgcg
     7201 ccgcagcgcc ggcagcagct gctcccggga ggcgtagagc acgtcggccg ccatgcggac
     7261 ggtggtcagc ggcgtgcgca gctcgtggct gacatcggag gtgaaccggc gttgcagtgc
     7321 gccgaactcc tcgagctggc ggatctggct ctggatactg ctcgccatct cgttgtagga
     7381 ctcgccgagc cgggctacgt cgtcctcacc ctgcacgggc atccgctcgt ccaggtgccc
     7441 gtcggcgaac cgctccgcga tctccgccgc ctgccgcacc ggccgcacga cctgccgcgt
     7501 caccagcccg gcgatcccgg cgagcagcag gaggaggatg agcccgccga cgatcagcgt
     7561 gctctgcacc cacccgagcg tgcgctgctc ggcggtcagc gggaacagca ggtagaactc
     7621 cagctcgccg ttggcggtac gaactggctg tccgacgatc agcgtcggga tgtcgacgcc
     7681 ctgctcggtg agcgtcacgt acttgctcga cagggtgccc gccgcgactg cgtcgcgcag
     7741 gtccgccggc acgtcctcga ccgggcctgc ggcgaccgcg cctgcgccgc ggctggcggc
     7801 gaggaccgcc cggaactcgc cggccgccgc ggccgacggc tcgtcgga
//
LOCUS       DASRSC010000373        10512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3601567,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000373 DASRSC010000000
VERSION     DASRSC010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10512
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3601567"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..424
                     /locus_tag="VFE39_18095"
     CDS             170..424
                     /locus_tag="VFE39_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET6260553.1"
                     /translation="MRPDIHPAYAPVVYQDRSTGDAFLTRSTATSSERISWADGNTYP
                     LIRVDITAYSHPFWTGAARVLDSAGAVEKFRRRYGDRARR"
     gene            complement(464..1315)
                     /locus_tag="VFE39_18100"
     CDS             complement(464..1315)
                     /locus_tag="VFE39_18100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET6260554.1"
                     /translation="MTIPRLLAVSDLHVRYPDNRSIVEGLQPTSDGDWLIVAGDVAEQ
                     VGEIEWALALLRERFARVIWVPGNHELWTRGKDPVPLRGGERYRLLVERCRTLDVLTP
                     EDPFPVWDGAGGPATVVPLFTLYDYSFLPAGTATSEEGLAAAYAAGVVCTDEHLLHPD
                     PFPDRAAWCAARLAESERRLAECDPEIPTVLVNHWPLTRLPTEVLRYPEFALWCGSER
                     TADWHVRYRATAVVYGHLHIPRVTWEDGVRFIEASLGYPREWRARDERNGTVVSEQLL
                     RQVLPTP"
     gene            1449..2771
                     /locus_tag="VFE39_18105"
     CDS             1449..2771
                     /locus_tag="VFE39_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HET6260555.1"
                     /translation="MSVTTKVRQGVRWGLRHALIRRTLERRMRDGDVSARLMFDEDVV
                     AEPFPFYDILREQGRLVDNGIVLNTAHHDVATAVLRSPDFGVVGGPSGQAPAALRFAE
                     RVAGHGPVGPVEPPSMLSVDPPDHTRYRKLVTRAFSARAVAALRSRTEEVAAELLDQM
                     AAKGRNADLVDDYASLLPATVIAEMLGAPAEMRRQFLEWGAGGAMSLDAGLSYRDFRL
                     SERHLEALQNWMLGHFEHIRRNPGDNILSALVHAHTEEGRLTEKEVTSIAVLLLAAGF
                     ETTVNLIGNGAAVLTAHPDQLALLRAEPQRWGNAVEEILRFDSPVQRTARFAGRDAEV
                     AGEQVRAGRIVVMLVGGANRDPAVFIDPHQFDITRENADGHLAFSSGIHYCLGAALAK
                     MEGEVGLRALFDRFPDLALDGPPRRRPTRVLRGYDAMPVRLSSATVNA"
     gene            complement(2787..3269)
                     /locus_tag="VFE39_18110"
     CDS             complement(2787..3269)
                     /locus_tag="VFE39_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HET6260556.1"
                     /translation="MSTDPPGFELPLRLLLGFRVLIDGLHAELARQGHPDVRPAHGFV
                     LQAVGHQGTTAVELGRRLGVTKQAAAKHVEALERLGYLRRAADAADARRKLIRLTDRG
                     MDCLARSARIFDELRAEWGATLGEDRMRALEADLRTMTPETPLRLDFPGWFGGARSDG
                     "
     gene            3329..3703
                     /locus_tag="VFE39_18115"
     CDS             3329..3703
                     /locus_tag="VFE39_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020662136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET6260557.1"
                     /translation="MPVIRHADSRRTTTPNAVMTTHASPSQGGSALAVWRVEMAAGAA
                     GPQHAFDAEQVWTALAGGATVELDGDAVAMHPGDTVVMPVGVQRRVLADASTGFTAVV
                     AAPAGARASVPGGEPVLPAWIA"
     gene            complement(3754..4803)
                     /locus_tag="VFE39_18120"
     CDS             complement(3754..4803)
                     /locus_tag="VFE39_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET6260558.1"
                     /translation="MSRSSEIDAAPPGPLTAWLRAEVPELPVGDRPVGVERISGGHSN
                     LTYRITDAAGGRWALRRPPTGMVLATAHDMGREWRFISALAPTPVPVAPPVAYCADTG
                     VIGAEFYLMKFVDGEVLGDEASGHRLESGGTRRTAGLDTVDVMAALHAIDPDAVGLGD
                     LRRSGNYLERQLRRWHRQVHESAVSDLSTVDAVHARLVERAAALPPSDVRIAHGDFRP
                     GNLAFGPDGRVRAVFDWELAALGDPLADLGWLIASWGRPGDAVPPTVPGPSLVDGYPD
                     RDELVARYAERSGRDVSQLDFYVAFARWRSACIGAGVHSRYAAGVMGAGEADAGAARL
                     ASLQRQAEAALAALG"
     gene            complement(4947..5357)
                     /locus_tag="VFE39_18125"
     CDS             complement(4947..5357)
                     /locus_tag="VFE39_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HET6260559.1"
                     /translation="MTGRHAFEYALLRVVPRIERGEAFNAGVLLYCRPLDYLGARVHL
                     DRHRLAALDPDADGVVIGRALDAVVAMCGAGRAEAARYSGPAGAEDRGRRFRRLTAPR
                     STIVQAGPVHTGLTADPKTELDRLLESLVLPLST"
     gene            complement(5354..6256)
                     /locus_tag="VFE39_18130"
     CDS             complement(5354..6256)
                     /locus_tag="VFE39_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HET6260560.1"
                     /translation="MATSPDRSGSRRAGGERVLRHVTAIRYVTPLREGGSLPGLMEAD
                     DLGTYVVKFHGAGQGRKALVAEIVSGELARGLGLPVPELVTVDLDPALGASEPDQEVQ
                     ELLRASPGLNLGMDFLPGALDLDPTAFAVGPDLAGRVLWFDALVGNVDRSWRNANMLF
                     WHRAPYLIDHGATLTFQHAWPGAEGWVRRPYDAANHVLLGSDPDLDAADDALAPQVTA
                     PLLQTAVDAVPDRWLVDEPGFDGPDDVRAAFVEQLLARRDAREAWLPGVRAAAAARKD
                     GQREPPSTRGSKPPYWVTHGQSRA"
     gene            6352..7749
                     /locus_tag="VFE39_18135"
     CDS             6352..7749
                     /locus_tag="VFE39_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005163435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HET6260561.1"
                     /translation="MTDVQPIARKSLVDEVPPPGRLLVLGLQHVLVMYAGCVAVPLIV
                     GSALHLDTRTIGLLVNADLVVAGIVTLIQTLGIGKILGVRLPIVTGATFTAVTPMILI
                     GQNFGLPGVYGSMIISGIFGMLIAVPFSRIIRFFPPVVTGSVITIIGLSLISAAAGLI
                     AGNDSTAPSYGDLSGLGLAGGIVLVIILITRFVRGFFGQIAVLTGLVIGALVAVPMGL
                     TDFSGVAAAPWFGVSTPFMFGPPQFPIAGVISMCVVMLVIFAESTADMLAVSEAVDEP
                     LEPARIARGLAADGLSGVLGGVLNSFPDTIFAQNVGLVQLTGVRSRFVPAVAGAILVL
                     LGLVPKFGEVVASLPGPVVGGAGLIMFATVTAVGIRTLHKVRFDGTNNLMIVAVSLGV
                     GMLPVVAPTFYAKVPETLGIIFGSSITSCVIVAFLLNLLFNHLSWGRPPGGPPLAHVG
                     SAAVIHHADEARTDP"
     gene            complement(7785..8294)
                     /locus_tag="VFE39_18140"
     CDS             complement(7785..8294)
                     /locus_tag="VFE39_18140"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HET6260562.1"
                     /translation="MNRSLPPPARDWALLLARVLIGVVMFAHGYQKLIINGIGRTTEG
                     FETLSIPLAIVSASFVTVVEFTCSVLLVAGTLTPVVCALMLVIMSGAAVFVHIPNGIF
                     VANGGWELVGVIAAGLIAITATGPGRFSVDHIVRTRQQQHQRDMQIPPRHAAEEEAVT
                     IWRSPVRGK"
     gene            8581..9828
                     /locus_tag="VFE39_18145"
     CDS             8581..9828
                     /locus_tag="VFE39_18145"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET6260563.1"
                     /translation="MVLRTAAAAPTTSFSTLSHLECSRDGSRHDADVVQGTSPNGAPL
                     LARYDLQRAAATVTPAQIAARPPDLWRYHELLPVRDPAHVVTLGEGMTPLLDLPRHGT
                     RLGVPRLRMKDEGLVPTGAFKARGAAVGISRAAELGVTGIAMPTNGNAGAAWASYAAR
                     AGMGALIAMPVDAPVITRRECVVAGAELYLVDGLIGDAGRLIAGAVARRGGFQDTSTL
                     REPYRIEGKKTMGYEIVEQLGWRAPEVILYPTGGGVGIIAIYKALLELRELGWLSGDL
                     PRLVAVQATGCAPIVDAFHAGHAESTAPADAHTVAFGITVPKALGDFLVLDAVRSTGG
                     TAVAVTDEELLAAQVALARDEGTWVCPEGAACFAAVGQLRESGWLDGTEDVVVLNTGT
                     GLIYPDTVQVDVPTLPKDGVIPG"
     gene            9863..>10512
                     /locus_tag="VFE39_18150"
     CDS             9863..>10512
                     /locus_tag="VFE39_18150"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET6260564.1"
                     /translation="MTAARAPGYGAVGAQHEEDSVMDGPLTLAIDVGGSGLKATVLSP
                     EGEMLSERERRETPYPCAPPVLLDELAALAATQPTYDRVSVGFPGAIRRGRVREVPAF
                     SRRGPGQQPDPELVSLWTGFELETALEQRFGKPTRVANDADVQGCAVISGHGMELVIT
                     LGTGVGCAVFFDGMLLPHMELSHGRFGHGHSIEVACGDNERQKIGKEHWRERVMDAL"
BASE COUNT         1533 a         3817 c         3662 g         1500 t
ORIGIN      
        1 ccggccggcg accgttccgt cgtcgtcgat cccacaaccg ggcgctgtgc acaagacggc
       61 cgtgaacccg gtccctgcgg cggtcggcat ccccacctcc gaaacaaaat gacaatcgtt
      121 ctcgttaagg aggacagtaa cagcccggag caccacaagg gagatcacga tgcgccccga
      181 catccacccc gcctacgcac ccgtcgtcta tcaggaccgg tccaccggcg acgcgttcct
      241 cacccgctcg accgccacct cgagcgagag gatctcctgg gcggacggca acacataccc
      301 gctgatcaga gtggacatca ccgcctactc gcacccgttc tggacggggg cggcgcgggt
      361 gctcgactcg gccggagcgg tcgagaagtt ccgccgccgc tacggcgacc gcgcgcggag
      421 gtgacgtcgc agggcctgcg tccggtggtc cggagcaggc ccgtcagggc gtcggcagca
      481 cctggcgcag cagctgttca ctgaccaccg tgccgttgcg ctcgtcgcgg gccctccact
      541 cccgcgggta gccgagagac gcctcgatga agcgcacgcc gtcctcccac gtgacacgcg
      601 ggatgtggag gtgcccgtag acgacagcgg tggcccggta ccggacgtgc cagtccgccg
      661 tgcgctccga gccgcaccag agcgcgaact ccgggtagcg cagcacctcc gtcggcagcc
      721 gggtgagcgg ccagtggttc accaacacgg tcgggatctc ggggtcgcac tccgcgagcc
      781 gacgttccga ctccgccagg cgcgcggcgc accaggccgc gcggtccgga aacgggtcag
      841 ggtggaggag gtgctcgtcc gtacacacca cccccgccgc gtacgccgcc gccaggccct
      901 cctccgaggt tgccgtacct gcgggcagga acgagtagtc gtagagcgtg aacagcggca
      961 cgaccgtcgc cggtccgcct gcaccgtccc agaccggaaa cggatcctct ggagtgagca
     1021 cgtcgagtgt gcggcaccgc tcgacgagca accggtagcg ctccccgccg cgcaatggca
     1081 caggatcctt gccgcgcgtc cacagctcgt ggttgcccgg gacccagatc acccgggcga
     1141 acctctcccg gaggagggcc agggcccact cgatttcgcc gacctgttcc gcgacgtcgc
     1201 ccgcgacgat cagccagtcc ccgtccgagg tcggttgcag gccttcgacg atgctgcggt
     1261 tgtccgggta gcggacgtgc aggtcgctga cggcgagcag cctcgggatg gtcacggaac
     1321 cagtcgtacc tgacgggagg tcagcttgcg cgccccggcc tcctgatcgc tgcggcctcc
     1381 tgatcacacc ggggtagctc ggccgacccg gaacggtaac gtcggtctaa agcgacgggg
     1441 ggtcgaatat gtcggtgacg acgaaggtgc gacagggtgt ccgctggggg ctgcggcatg
     1501 cactgatccg gcgcacgctc gagcggagga tgcgggacgg cgacgtgagc gcccggctca
     1561 tgttcgacga ggacgtggtg gccgagccgt tccccttcta cgacatcctg cgtgagcagg
     1621 ggcgtctggt cgacaacggc atcgtcctca acaccgcaca ccacgacgtc gccacggcgg
     1681 tgctgcgcag ccccgacttc ggggtggtcg gcggcccgag cggccaggcc ccggcagcac
     1741 tgcggttcgc ggagcgcgtc gcggggcacg ggccggtcgg gccggtggaa ccgccgtcga
     1801 tgctttccgt cgacccaccc gaccacaccc gctaccgcaa gctcgtcacc agggcgttca
     1861 gcgcccgcgc ggtggccgcc ctgcgcagcc gtacggagga ggtcgccgcc gagctgctcg
     1921 accagatggc ggcgaagggc cgcaatgccg acctcgtcga cgactacgcc agcctcctgc
     1981 cggccaccgt gatcgccgag atgctgggcg cgcccgccga gatgcgccgg cagttcctgg
     2041 agtggggagc gggcggcgcg atgtcgctgg acgccgggct ctcgtaccgc gacttccggc
     2101 tctccgagcg gcacctcgag gccctgcaga actggatgct cggccacttc gagcacatcc
     2161 gccgcaaccc cggcgacaac attctctccg cgctcgtgca cgcacacacg gaggaggggc
     2221 ggctcaccga gaaagaggtc accagcatcg cggtgctgct tctcgccgcg gggttcgaga
     2281 ccacggtcaa cctcatcggc aacggcgccg cggtgctcac cgcccacccc gaccagctcg
     2341 ccctgctgcg ggccgagccg cagcgctggg gcaacgccgt cgaggagatc ctccgcttcg
     2401 actcgcccgt gcagcgcacc gcgcggttcg cgggccgcga cgccgaggtg gccggcgagc
     2461 aggtccgggc cggtcggatc gtcgtgatgc tggtgggtgg cgccaaccgc gacccggcgg
     2521 tcttcatcga tcctcaccaa ttcgacatca cgcgagagaa cgcggacggt cacctcgctt
     2581 tttccagcgg catccactac tgcctgggcg cggcactcgc gaagatggag ggcgaggtgg
     2641 ggctccgcgc gttgttcgac cgcttccccg acctggcgct cgacggtccc ccacgccgcc
     2701 gacccacccg cgtcctgcgt gggtacgacg cgatgccggt gcgcctgtcc tccgccaccg
     2761 tcaacgcctg acgcggccgg ccgcggtcat ccgtccgatc tcgcgccgcc gaaccacccg
     2821 gggaagtcga ggcgcaatgg cgtctcggga gtcatcgtgc gcaggtcggc ctcgagtgca
     2881 cgcatgcggt cctcgcccag cgtggcgccc cattcggcgc gcagctcgtc gaagatccgg
     2941 gcggagcggg cgaggcagtc catcccacgg tcggtaagcc tgatcagttt gcggcgcgcg
     3001 tcggcggcgt cggctgcacg gcgcaggtaa ccgaggcgct ccagcgcctc gacgtgtttg
     3061 gcggcagcct gcttggtcac gcccagtcga cggccgagct cgacggccgt ggtgccctgg
     3121 tgaccaaccg cctgcaggac gaagccgtgc gccgggcgaa cgtcgggatg accctgacgg
     3181 gcgagttcgg cgtgcagccc gtcgatcagt acgcggaacc cgagcagcag ccgcagcggc
     3241 agctcgaaac ccggtggatc agttgacatg ttcgacaacc aggttcacta ttccgacaac
     3301 cacattgtct atcttagggg agcccgtcat gcccgtcatc cgccacgccg acagccgccg
     3361 gaccaccacc ccgaacgccg tcatgaccac ccatgcgtcc cccagccagg gcgggagcgc
     3421 gctggccgtc tggcgggtgg agatggcggc gggcgcagca ggcccgcagc acgcgttcga
     3481 cgccgagcag gtatggacgg cactggccgg cggcgccacc gtcgagctgg acggagacgc
     3541 ggtcgccatg catccgggcg acaccgtggt gatgccggtc ggcgtgcagc gtcgcgtgct
     3601 ggccgacgcc tcgaccgggt tcaccgcagt cgtcgccgcg cccgcaggtg ccagggcgtc
     3661 ggtgccgggc ggtgagccgg tgctgccggc ctggatcgcc tgacccgcgc cgcggccgcg
     3721 gtctgccacc ggtgctcggg ccgcccgggc ctgtcagccg agcgcggcca gtgcggcttc
     3781 ggcctggcgt tgcagcgacg ccaggcgggc cgccccggcg tccgcctcgc ctgcgcccat
     3841 gacgcctgct gcgtagcggc tgtggacacc ggcgccgatg caggccgagc gccaacgggc
     3901 gaaggccacg tagaagtcga gctgggagac gtcgcggccg gagcgctcgg cgtagcgggc
     3961 cacgagctcg tcgcgatcgg gatacccgtc caccaggctg ggccccggga ccgtgggcgg
     4021 caccgcgtcg ccgggacggc cccaggatgc gatcagccag ccgaggtcgg ccagcgggtc
     4081 gccgagggcg gccagctccc agtcgaacac cgcgcgcacc cggccgtccg gcccgaaggc
     4141 gaggttgccc ggccggaaat caccgtgcgc gatccggacg tcggacgggg gcagcgccgc
     4201 ggcccgctcg acgagccgag catggacggc gtccaccgtg gacaggtccg acaccgccga
     4261 ctcgtgcacc tgccggtgcc accgccgcag ctgccgctcc agatagttgc cggagcgccg
     4321 caggtcgccc aggccgaccg catcgggatc gatcgcgtgc agcgccgcca tcacgtcgac
     4381 ggtgtcgagg cctgcggtac gccgggtacc gccggactcg agccggtggc ccgacgcctc
     4441 gtcaccgagc acctcgccat cgacgaactt catgagatag aactcggcac cgatgacgcc
     4501 cgtgtcggcg cagtacgcga ccggtggcgc caccggtacc ggtgtgggcg ccaacgccga
     4561 gatgaagcgc cactcgcggc ccatgtcgtg tgcggtggcg agcaccatgc cggtcggcgg
     4621 gcgtcgcaac gcccacctgc cgcccgcggc gtcggtgatc cggtaggtga ggttggaatg
     4681 cccaccggag atgcgctcga cgccgacggg cctgtccccc accggcagct cgggcacctc
     4741 cgcgcgcagc cacgcggtca gcgggccagg gggtgcagcg tcaatctccg atgatcggct
     4801 caccacccca tccaatctcc ccgcccccgg aaggccacgc ccgccccgcc cgcgagtcgc
     4861 ccgatccacg cccgcgagtc gccctgctcc cgcccccgag tcgcccgatc cccgcccggg
     4921 ggccgggccc gagggcgcac cgggcgtcac gtcgacagcg ggaggaccag agactccagg
     4981 aggcggtcga gctcggtctt cgggtcggcg gtgagcccgg tgtgcacggg gccggcctgc
     5041 acgatcgtgc tgcgcggagc ggtcagccgc cggaaccgcc tgccgcggtc ctccgccccc
     5101 gcaggccccg aataccgagc cgcctcggcg cggccggcgc cgcacatcgc caccaccgcg
     5161 tcgagggcgc ggccgatcac caccccgtcg gcatcgggat cgagtgcggc gagccggtgc
     5221 cggtcgaggt gcacacgcgc cccgaggtag tccagtggac ggcagtacag gagcacaccg
     5281 gcgttgaacg cctcaccgcg ctcgatccgc ggcaccaccc gcagcagggc gtactcgaag
     5341 gcgtggcggc ccgtcatgcc cggctctgcc cgtgcgtgac ccagtacggc ggcttcgatc
     5401 cgcgggtgga cggcggttcg cgctgcccgt ccttgcgcgc agcggcggcc gcccgcacac
     5461 ccggcagcca cgcttcgcgc gcatcacgcc gcgccaggag ctgctcgacg aaggccgccc
     5521 ggacgtcgtc agggccatcg aagccgggct cgtcgacgag ccatcggtcg ggcacggcgt
     5581 caaccgccgt ctgcaacagc ggagctgtga cctgcggggc gagggcgtcg tcggccgcgt
     5641 cgaggtcggg gtcggagccg aggagcacgt gattggccgc gtcatagggc ctccgcaccc
     5701 agccctccgc ccccggccag gcgtgttgga acgtcagcgt ggcgccatgg tcgatgaggt
     5761 agggcgcgcg gtgccagaac agcatgttgg cgttgcgcca cgagcggtcg acgttgccga
     5821 cgagcgcgtc gaaccacagc acccgccccg cgagatccgg acccacggcg aacgcggtgg
     5881 ggtcgaggtc gagcgcgccc ggtaggaagt ccatgccgag gttgagcccc gggctggccc
     5941 gcaacagctc ctgtacctcc tggtcgggct ccgaggctcc cagcgccggg tccaggtcga
     6001 cggtgaccag ctccggcact ggcaggccca gcccgcgcgc cagctccccg gaaacgatct
     6061 ccgcgacgag ggccttgcga ccctgcccgg ccccgtggaa cttcacgacg taggtgccga
     6121 ggtcgtcggc ctccatgagc cctggcagcg acccaccctc gcgcaacggc gtgacgtagc
     6181 ggatcgccgt gacgtgccgc agcacccgct caccccctgc tcgacgcgac ccggatcggt
     6241 cgggcgacgt ggccatcctc gcaccacggc gcacccgctt ttctggcgcc gtcacgcctc
     6301 gttaacgagt gcgatctacg gtccctcaca acgatgacga gaggcgggcg catgaccgac
     6361 gtgcaaccca tcgccaggaa gagcctggtc gacgaggtgc caccaccggg ccgcctcctg
     6421 gtgctcggcc tgcagcacgt gctcgtgatg tacgccggat gtgtggccgt gccgctgatc
     6481 gtcgggagcg cgctgcacct ggacacccgc acgatcgggc tcctggtgaa cgccgacctc
     6541 gttgtggcgg ggatcgtcac gctgatccag accctcggca tcgggaagat cctcggcgtg
     6601 cggctgccga tcgtcaccgg cgccaccttc accgccgtca ccccgatgat cctgatcgga
     6661 cagaacttcg gccttcccgg cgtctacggc tcgatgatca tctccgggat cttcgggatg
     6721 ttgatcgctg tgccgttctc gcggatcatc cggttcttcc cgcccgtcgt gacgggcagc
     6781 gtcatcacga tcatcgggct atcgctgatc tcggcagcgg ccggactgat cgcgggcaac
     6841 gactcgaccg ccccgagcta cggcgacctc tccgggctcg ggctcgccgg ggggatcgtg
     6901 ctggtcatca tcctgatcac ccggttcgtg cggggcttct tcggtcagat cgcggtgctc
     6961 accggactcg tgatcggggc gctggtggcg gtcccgatgg gcctcacgga cttctccgga
     7021 gtcgccgccg caccctggtt cggagtgtcc acgccattca tgttcgggcc gccgcagttc
     7081 ccgatcgccg gcgtgatctc gatgtgcgtc gtgatgctgg tgatcttcgc cgagtcgacg
     7141 gcggacatgc tcgccgtcag cgaggccgtc gacgagccgc tggagcccgc ccggatcgcg
     7201 cgcggtcttg ccgccgacgg gctctccggc gtgctcggcg gtgtgctcaa ctcgttcccc
     7261 gacacgatct tcgcccagaa cgtcgggctc gtgcagctca ccggcgtccg cagccggttc
     7321 gtccccgccg tggccggcgc gatcctcgtg ctgctgggcc tcgtgcccaa gttcggcgag
     7381 gtggtggcct cgctgcccgg tccggtcgtc ggcggcgccg gcctgatcat gttcgcgacg
     7441 gtgacggcag tcggcatccg caccctgcac aaggtgcgct tcgacggcac gaacaacctc
     7501 atgatcgtgg ccgtatcgct gggcgtcggg atgcttcccg tggtcgcgcc gacgttctac
     7561 gcgaaggtgc cggagaccct cgggatcatc ttcggctcct cgatcacctc gtgcgtgatc
     7621 gtggcgtttc tgctgaacct gctgttcaac cacctgtcgt ggggacggcc gccgggcggc
     7681 ccgccgctgg cccacgtcgg atcggcagcc gtcatccacc atgcggacga ggcgagaacc
     7741 gacccctgat caccccgtca cagtgcggtc gcagggttgt tctcctactt gccccgcacc
     7801 ggactccgcc agatcgtgac ggcctcttcc tcagcagcgt gtcgcggggg aatctgcatg
     7861 tcgcgctggt gctgctgctg ccgggtgcgg acgatgtggt cgacgctgaa gcggccgggc
     7921 cccgtggcgg tgatggcgat caggccggcc gcgatcacgc cgacgagctc ccacccgccg
     7981 ttggcgacga agatgccgtt ggggatgtgc acgaagaccg ccgcaccact catgatcacc
     8041 agcatcagcg cgcacaccac cggcgtgagc gttcccgcga ccagcagcac gctgcaggtg
     8101 aactcgacca ccgtcacgaa cgaggccgag acgatcgcga gcgggatgct cagggtctcg
     8161 aacccttccg tggtgcggcc gatgccgttg atgatcaact tctgataccc gtgggcgaac
     8221 atcacgacgc cgatgaggac acgcgccagc agaagtgccc agtcgcgcgc cggcgggggc
     8281 agggaacggt tcatcggatc ctccgcagcg ctgtgtgatc aaatgagctc ggtaagtgac
     8341 catcaaagtc aacctcacgt agccacctga tcacggaagg tcatacgatt ccctcgacgg
     8401 ccgggcatcg ctgcgcgatg agcagtcggc ataacggtca gacgggcaca accgaacgtg
     8461 cttgcaccga cggtcgcgcg ggacgcacca ctcgccgtga tcttcgtcag gtggtgatca
     8521 tcgtcaccgc tgcggcgcac cacagttgac gcgctcgtcg cagcaggatt gcctgccagc
     8581 gtggttctcc gcacggctgc cgccgcaccg acgacgtcct tctccaccct ctcgcacctc
     8641 gaatgctccc gcgacgggtc ccgccacgac gctgatgtcg tccagggcac ctcgccgaac
     8701 ggtgccccac tgctcgcccg ttacgacctg caacgggccg ctgccaccgt cacaccggcg
     8761 cagatcgccg cccgtccacc ggacctgtgg cgctaccacg agctgctgcc cgtgcgggat
     8821 ccggcgcacg tcgtcacgct cggggagggc atgacgccgc tgctcgacct accccgccac
     8881 ggcacccggc tgggcgtccc ccgcctccgc atgaaggacg aggggctggt gcccaccgga
     8941 gcgttcaagg cgcgcggcgc cgcggtcggg atctcgcgtg ccgctgagct cggcgtcacc
     9001 gggatcgcga tgccgacgaa cggcaacgcc ggcgccgcgt gggccagcta cgcggcgcgc
     9061 gccggcatgg gtgcgctgat cgcgatgccc gtcgacgccc cggtgatcac ccggcgcgag
     9121 tgcgtggtgg cgggtgccga gctgtacctg gtcgacgggc tgatcggcga cgccgggcgg
     9181 ctgatcgccg gggcggtcgc gcggcgcggc ggcttccagg acacgtcgac gctgcgcgag
     9241 ccgtaccgca tcgaaggcaa gaagacgatg ggctacgaga tcgtcgagca gctgggctgg
     9301 cgtgcaccgg aggtcatcct ctatcccacc gggggcggcg tcgggatcat cgccatctac
     9361 aaggcgctgc tcgagctgcg tgagctggga tggctgtccg gcgacctgcc gcggctcgtg
     9421 gcggtccagg cgacggggtg cgccccgatc gtcgacgcgt tccacgccgg acacgccgag
     9481 agcacggcac ccgccgacgc gcacaccgtc gcgttcggga tcaccgtgcc gaaggcgctg
     9541 ggtgacttcc tcgtgctcga cgccgtgcgg tccacaggtg gcaccgccgt ggccgtcacc
     9601 gacgaggagc tgctcgccgc acaggtggcg ctggcccgcg acgagggcac gtgggtctgc
     9661 ccggaggggg ccgcgtgctt cgccgcggtg gggcagctgc gggagtcggg ctggctcgac
     9721 ggcaccgagg acgtggtcgt cctcaacacc gggaccgggc tgatctaccc ggacacggtg
     9781 caggtcgacg tgccgaccct gcccaaggac ggtgtgatcc ccggctgacg ggcagccctg
     9841 atcttcgtgc ggagtactcg cgatgacagc cgcgcgcgcg cccggctacg gtgccgtggg
     9901 ggcacagcac gaggaggatt cggtcatgga cggaccgctc accctggcta tcgacgtcgg
     9961 cggctcaggg ctcaaggcca cggtgctgtc gccggagggc gagatgctgt ccgagcgcga
    10021 gcgccgcgag acgccgtacc cgtgcgcgcc ccccgtgctg ctcgacgagc tcgccgctct
    10081 cgctgccacg cagcccacct acgaccgggt gtcggtcggc ttccccgggg cgatccgccg
    10141 cggccgggtc cgcgaggtac cggccttctc ccggcgcggg cccggccagc agccggaccc
    10201 cgaactggtg tcgctgtgga ccggcttcga gctggagacg gcgctcgagc agcgcttcgg
    10261 caagccgacg cgggtggcca acgacgccga cgtgcagggc tgcgccgtca tctccggcca
    10321 cggcatggag ctggtgatca cgctcggcac cggcgtcggg tgcgcggtgt tcttcgacgg
    10381 catgctgctg ccgcacatgg agctctcgca cggccgcttc gggcacgggc actccatcga
    10441 ggtggcctgc ggcgacaacg aacggcagaa gatcggcaag gagcactggc gcgagcgtgt
    10501 gatggacgct ct
//
LOCUS       DASRSC010000374        14439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1962511,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000374 DASRSC010000000
VERSION     DASRSC010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 14439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14439
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1962511"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VFE39_18155"
     CDS             <1..235
                     /locus_tag="VFE39_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HET6260565.1"
                     /translation="DLIEDGCAVLAKEGRMMAYRHRGFWQPADTVKERTALEAAYQGG
                     TRPWMLWEAAGEERDRDPLQAAIAAMSDANIER"
     gene            256..927
                     /locus_tag="VFE39_18160"
     CDS             256..927
                     /locus_tag="VFE39_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HET6260566.1"
                     /translation="MLSLLPERLDRILLLGAHCDDIAIGAGGSLIELCRAHPGVTVSA
                     LVLTGGGSLREEEERAALTSFCPGAQLEVAVLDLPDGRVPTRWERAKLAVEELRSLGE
                     PDVIFAPAPHDAHQDHRTLAKLVPTVFRDHLTLGYEILKWEGDLAQPTVYLPLAEPVL
                     REKVAKLHEHYGSQRDRSWFDAETFGGLARIRGVQCHARYAEAFHASKLVLGVAPLTG
                     VDPVD"
     gene            983..2008
                     /locus_tag="VFE39_18165"
     CDS             983..2008
                     /locus_tag="VFE39_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET6260567.1"
                     /translation="MRILLTGHQGYLGTVMAPILTAAGHELTGLDSGLFADCVLGSLD
                     TPDVPGLRTDLRDVRVEQLQGFDAVVHMAALSNDPLGSLAPEITYDITHHASTRLARL
                     AKAAGVTRFAYASTCSVYGAQSGDELVDEDAPLKPVTPYAISKVRVEDDLAELADSDF
                     VPFSLRNATAFGFSPRLRADIVLNNLVGRAILTGKVTVLSDGTPWRPLAHAEDIAGAV
                     VAALAAPADVVRARAFNVGTEKNNRTVAEIAEAVVEAVPGAELEITGEAGNDPRSYRV
                     DFSRARKELEFEAQWTIPDGAQQLATEYRDRGLTQDAFDNRFTRLAVLSRRQQAGELD
                     EAMRVIA"
     gene            2076..2363
                     /locus_tag="VFE39_18170"
     CDS             2076..2363
                     /locus_tag="VFE39_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HET6260568.1"
                     /translation="MRGPSMCRNIRTLHNFEPPATRDEVHGAALQYVRKISGSTQPSQ
                     ANADAFARAVEAVSAATHRLLDELVTAAPPKNREVEAVKARERSAARYSTG"
     gene            complement(2384..3274)
                     /locus_tag="VFE39_18175"
     CDS             complement(2384..3274)
                     /locus_tag="VFE39_18175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260569.1"
                     /translation="MITLYLNRQIADDRRRESLFSGDFYLYTGQPGAAALVKHAESLI
                     ADTFGDLDPERAQFEMTVDEFIARVGPLKSEFTNGQRTKELCQEFAVTIGVDPDRTYF
                     DIPRLRVIPAENFLTAGVSYNYKAHRDMWYGHPQQLVNYWVPVFPVVGDNVMSMYTDY
                     FDKPVKNGSNAYDYDEWVAKHRHAAAEKITTDDRPHPLPLEDIDSRGEIRIAGSAGDV
                     MMFSSNHLHASAPNLSGVTRFSYDLRTINIDDVRAGNGPRNVDSGATGTTLGDFLRVS
                     DLAPLQADEFADAVAASPKH"
     gene            complement(3417..4646)
                     /locus_tag="VFE39_18180"
     CDS             complement(3417..4646)
                     /locus_tag="VFE39_18180"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020664749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HET6260570.1"
                     /translation="MTPTRYAVATAVAAALGLAGCTAPEASPIERGSASTTPAAAPLA
                     PARTGTPELRVSVVAAGLSHVWDLGFLPDGRVLLTERAGRIALLSSTQPGATVTPVTA
                     DLSDVYVRGEGGLMGMVVHPDFAQTRRFTTCQTHMKAGTPTDVRLVTWQLSADGTAAS
                     RVADPLVGGMPINPSGRHSGCRPTLTADGALLVGTGDTARGAIAQDLASLGGKVLRID
                     LDTGGPAPGNPYADAQNPAQRLIWSYGHRNVQGVAVTPDTGKAYSAEHGPTTDDEVNL
                     LGAGANYGWDPSQGGTVGGYDESVPMTDLQRFPDAVPAAWSSGNPVEAVSGAAFLSGR
                     QWGDLDGVLAVGALRGEKLLLMQLGPGGAVANVTIPAPLDGAFGRLRAVRVAPDGALF
                     VSTSNGTDDRVLRIDPV"
     gene            4791..8159
                     /locus_tag="VFE39_18185"
     CDS             4791..8159
                     /locus_tag="VFE39_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HET6260571.1"
                     /translation="MPRSYLRFPHLHADTLVFVAENDVWTAPVSGGRAYRLTADDVPV
                     AQPRLSPDGAQVAWTSWREGAPEVFVADLDGGGARRLSYWADPRTQTIGWTPAGEVLA
                     VSAAGQASPRRGWAYAIPATGGTPRRLDFGPVSDVALPAGGGPAVVVTSLMTRDMAWW
                     KRYRGGTAGKLWWDAAGSGEFVRLAADLDGQLAAPMLIGSGDGLRIAFISDHEGWGNL
                     YSLGADGAGLRRHTDHGGPDAPAFYVRHASTDGERIVYESAGELWILDSLDSAAPRRL
                     DVRLGGPRTARDPFRVRTADELSWVAPDRTGRTSIAQVRGTVHRLTHRDGPARTLLAE
                     PGARARLARPLGDDRAVWVDDVEGEDAICIAPLDERADGGQPLRRYGAGAVGRVLELA
                     AAPDGSKLALAAHDGRLLVLELAGSRAGELRELARGADGEISDLCWSPDSAWLAYSDP
                     VEAGLSRIMMARLADDTLVAVTEPRFADTDPTFTFDGKYLAFLSRRSFDPIYDQHSFD
                     LTFPASWRPFLVPLAARTPSPFGASPDGRPVSAQDEGPEDPPAPDPSDPSAEPTPDAG
                     GEGTGKPEKKKGDPPPEVVVDVEGLAARVVPVPVVAARYSGMQAGKDCLLWYRSPVSG
                     VLGDTRAGTDEKAERPVLERYDLVRRKLDVIADPVSSFAVSGDGSRLVVRDGGALRVL
                     RTDRSGSGAPGEGDGDEFEIDTRRIVVTVDPTAEWRQMFDEAGRLMRDHFWTEDMAGV
                     DWAAELARYRPLVDAVGSHDDLVDLMWELQGELGTSHAYVMGHGAGDHSGQPGLLGAD
                     LEPGEPDGWRVVRVLPPETSAPAARSPLSGPGVDVRAGDLILEVGGKPVDPAYGPAPL
                     LVSRAGELVELTVRSGPDREDAGEVRRVVVRPLGSETSLRYQDWVAGRRAFVADRSEG
                     RLGYLHVPDMMAPGWAQLHRDLTRETSREGLILDVRGNSGGHTSQLVVEKLARKVIGW
                     DLPRHRQPTTYPEDAPRGPVVALADELSGSDGDIVTAAIKRLGIGPVVGVRTWGGVVG
                     IDSRYGLVDGTRVTQPRYAFWFDDAHWGVENHGVDPDVEVVIAPQDWVAGRDPQLERA
                     VDMALEALAERPAVRAPDVASRPSRRRPDLPPRP"
     gene            complement(8255..9421)
                     /locus_tag="VFE39_18190"
     CDS             complement(8255..9421)
                     /locus_tag="VFE39_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase family protein"
                     /protein_id="HET6260572.1"
                     /translation="MHYHPQQPDRTGDGEIHDNYGPEAAFAVRAEAGLPTTKWEEEVA
                     RGLELGLQGADSIVDRRIPTFSRGELPHFAGINTFLKAPYVEDVRRCGEYDVAVLGAP
                     FDGGTTYRSGTRFGPQGIRKISALYGPYSFELGVDLRESITIGDLGDIFTIPGNIEKT
                     FDQITKAVSHVYESGAFPVVLGGDHSIGYPTTRGVAPHLNGGNLGIIHFDRHVDTQET
                     DLDERMHTTPWFHATDLPNVPAKNLVQIGIGGWQAPRPGVKVGRERGTTIMTVTDCVE
                     MGIEDAAQRALDVAWDGADAVWLSFDVDCLDAAFVPGTGWPEPGGFLPREVLKFIQII
                     ADARPLAGIEVVECSPPYDNAEITALIATRVICDTLGCLVRSGHLPAARKKPAP"
     regulatory      complement(9476..9583)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00442"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="guanidine-I (ykkC/yxkD leader) riboswitch;
                     Guanidine-I riboswitches control genes whose products are
                     involved in modification or pumping out guanidine as a
                     toxic compound from bacteria; This is class of guanidine
                     riboswitches is based on the ykkC/yxkD leader; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="guanidine"
                     /db_xref="RFAM:RF00442"
     gene            9815..13381
                     /gene="mfd"
                     /locus_tag="VFE39_18195"
     CDS             9815..13381
                     /gene="mfd"
                     /locus_tag="VFE39_18195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005459230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET6260573.1"
                     /translation="MAKLSGLLRSSLSDPDLRGVVEAARDTRADEVPALRIEGPGALR
                     PFLAAAFGTAADAGGAGHTVLAVTATDREAEDLGAAAGDLLGRAAVAVLPSWETLPHE
                     RLSPRPDTVGRRLEIFRRLANPETAPRVVVAAARSLIQPIAPGLGRLDPVQLRVGDIA
                     DFDALLVRLVELAYTRVEMVTARGEFAVRGGIIDVFPPTAEHPVRVELWGDEVSELRY
                     FAVADQRSVGPVEELVAPGCRELLLTEPVRERAAALARAHENNPPLRELLEHLAEGIP
                     SEGMESLVPALVGGELELLTDLLPADGLVLLADPERIRTRSADLVRTGQEFLEASWFA
                     AGTGGAAPIDVGASAYRDLGEVLDHASATGRPVVSLSPLLSGGDDALAPTVHEVESYR
                     GDTDRALVDLRAHVATGGTAVLVVAGQGTAQRSLEQLREADVPAKLVEELADIPEPGL
                     VTVTCGRLAEGFTAHGLVVLTEADLTGNRAAGTEPRKLASKRRNAVDLVTLQPGDYVV
                     HSQHGIGKFVEMRERTVGGATREYLVLEYASSKRGQPADRLFVPTDSLDEISRYVGGE
                     VPTLNKLGGGDWAKTKGRARKAVRQIAAQLVQLYAARQSAPGHAFAKDTPWQRELEDA
                     FPFTETPDQLAAIEEVKRDMEQPVPMDRVISGDVGFGKTEIAVRAAFKAVQDGKQVAV
                     LVPTTLLATQHLQTFSDRMRAFPVTVKGLSRFTDNAEAKQTIEGLADGTVDIVVGTHR
                     LLQTGIRWKDLGLVIVDEEQRFGVEHKEHITALRTHVDVLTLSATPIPRTLEMSLAGI
                     REMSTIATPPEDRHPTLTYVGAYDEKQVGAAIRRELLRDGQVFYVHNRVSTIDRAARR
                     IRELVPEARVAIAHGQMNEDLLERTVNGFWHNEFDVLVCTTIVENGLDISNANTLIVE
                     RSDTLGLSQLHQLRGRVGRGRERGYAYFLFPPEHPLTETAHDRLATIAQHSELGAGTA
                     VAMKDLEIRGAGNILGAEQSGHIAGVGFDLYIRLVGEAVAAFRQQAGAGDGEDVEQLA
                     DVRVDLPVDAHVPHDYVTGERLRLEVYRKIAEAGEAADLDAIVEEITDRYGEPPTPVR
                     NLLAVAAFRQTCRALGVAEVAVAGNGVRIGPVDLPDSATLRLKRLYPKAVYRPAARAV
                     LVPKPVESGRIGGAPLRDVELLDWCTKLLRDLLAPAKVPASV"
     gene            13604..14026
                     /locus_tag="VFE39_18200"
     CDS             13604..14026
                     /locus_tag="VFE39_18200"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HET6260574.1"
                     /translation="MYGPELATYGQRVGGFLIDVGIPTGLLVAVLIAALATRDLTMIM
                     VVYGLSAAMLTAFVLWNSGYRQGRSGQSIGKRALGVRLVTAETGQPVGFGRAVGRQFA
                     HLLDGIPFSLGYLWPLWDEQRQTFADKVCCTLVVQADM"
     gene            14094..>14439
                     /locus_tag="VFE39_18205"
     CDS             14094..>14439
                     /locus_tag="VFE39_18205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260575.1"
                     /translation="MRKQVGRVAAAVAVVGAMVSGCGLDQAGSAVIVGNDAVPLGRVQ
                     SQLDVALGKTDQIAQLTAQGGTPADLSRSIVTREVLHDLLQRRAAEDGIVVTDAQVEA
                     ELAGSGGADAVVN"
BASE COUNT         1993 a         5112 c         5283 g         2051 t
ORIGIN      
        1 cgacctgatc gaggacgggt gtgcggtgct ggcgaaggag ggccggatga tggcgtatcg
       61 ccaccgcggg ttctggcagc cggccgacac ggtcaaggag cgcaccgcgc tggaggcggc
      121 ctaccagggc ggcacccggc cgtggatgct ctgggaggcc gccggcgagg agcgcgaccg
      181 tgatcccctg caggctgcta tcgctgccat gagcgacgcc aacatcgagc gctgacgccc
      241 ggtgccccga gaatcatgct gagcctcctg ccggagcgtc tcgaccggat cctgctgctc
      301 ggcgcccact gcgacgacat cgcgatcggc gccggcggct ccctgatcga gctgtgccgc
      361 gcgcaccccg gcgtcaccgt ctcggcgctg gtgctcaccg ggggcggctc cctgcgggag
      421 gaggaggagc gcgcggcgct cacctcgttc tgtcccgggg cccagctcga ggtcgcggtc
      481 ctcgacctgc ccgatggacg cgtcccgacc cggtgggagc gggccaagct cgccgtcgag
      541 gagctgcgtt cccttggcga gccggacgtc atcttcgccc cggccccgca cgacgcccac
      601 caggaccacc ggacgctcgc gaagctcgtc cccaccgtgt tccgcgacca tctcacgctc
      661 ggctacgaga tcctcaagtg ggagggcgac ctcgcgcagc ccaccgtcta cctgccgctg
      721 gccgaaccgg tgctccggga gaaggtggcc aagctgcacg agcactacgg ctcccaacgc
      781 gaccgcagct ggttcgacgc cgagacgttc ggcgggctgg cccggatccg cggggtgcag
      841 tgccacgcgc ggtacgcgga ggcgttccac gcgtccaagc tggtgctcgg cgtggccccc
      901 ctcaccgggg tcgacccggt ggattgaacc tccccgccat ccacgacgac cactcggacg
      961 accactcgaa gggatcctcc acgtgcgaat tctgctgacc ggccaccagg gctacctggg
     1021 caccgtgatg gccccgatcc tcaccgcggc aggccatgag ttgaccgggc tggacagcgg
     1081 cctgttcgcc gactgtgtcc tcgggtcgct ggacaccccg gacgtgccgg gtctgcgcac
     1141 cgacctgcgg gacgtaaggg tcgagcagct gcagggcttc gacgccgtcg tccacatggc
     1201 tgcgctgtcc aacgacccgc tggggtcgct ggcacccgag atcacctacg acatcaccca
     1261 ccacgcctcc acacggctgg cgcggcttgc caaggcggcg ggcgtcaccc gcttcgccta
     1321 cgcgtccacc tgctcggtct acggtgctca gtccggtgac gagctcgtcg acgaggacgc
     1381 cccgctcaag cccgtcaccc cgtacgcgat ctcgaaggtg cgtgtcgagg acgacctggc
     1441 cgagctcgcc gacagcgact tcgtgccttt ctccctgcgc aacgccaccg cgttcgggtt
     1501 ctccccgcgg ctgcgcgccg acatcgtgct caacaatctc gtcggccgcg ccatcctcac
     1561 gggcaaggtc accgtgctct ccgacggcac tccgtggcgc ccgctcgcac acgccgagga
     1621 catcgcaggc gcagtcgtgg ccgcactcgc cgcgcctgcc gacgtcgtgc gcgcacgggc
     1681 cttcaacgtg ggcaccgaga agaacaaccg gaccgtcgcc gagatcgccg aggccgtggt
     1741 cgaggcagtg cccggcgccg agctggagat caccggagag gccggcaacg acccgcgctc
     1801 ctaccgggtg gacttctccc gggcgcgcaa ggagctcgag ttcgaggcgc agtggaccat
     1861 tccggacggc gcgcagcagc tcgccacgga gtaccgcgac cgcggactca cgcaggacgc
     1921 gttcgacaac cggttcactc ggctcgcggt cctgagccgt cgccagcagg cgggcgagct
     1981 ggacgaggcc atgcgcgtca tcgcctgaca gcatccgagc gctcgaacga gcggcattct
     2041 cgtccagtag gttcggacgg gactgccgtt cgttcgtgag ggggccttcc atgtgccgca
     2101 acatccggac tctgcacaac ttcgagccgc ccgccacccg cgacgaggtg cacggcgccg
     2161 ccctgcagta cgtccgcaag atcagcgggt cgacccagcc gtcacaggcc aacgcggacg
     2221 cgttcgcccg ggccgtggag gccgtctccg cagcaacgca tcgcctgctc gacgagctgg
     2281 tgacggccgc gccgccgaag aaccgcgagg tggaggcggt taaggcccgg gagcgctccg
     2341 ccgcgcgcta ctccacaggc tgaggcgcgc ggcagagccc tgttcagtgc ttgggggagg
     2401 cggccacggc gtcggcgaac tcgtcggcct gcagcggcgc gaggtcggac actcggagga
     2461 agtcaccgag ggtggtaccg gtggcgccgc tgtcgacgtt gcggggaccg ttgccggcgc
     2521 ggacgtcgtc gatgttgatg gtgcgcaggt cgtaggagaa gcgcgtgacg ccggacaggt
     2581 tgggcgcgga cgcgtggagg tggttggagg agaacatcat cacgtcgcct gcactccccg
     2641 cgatgcggat ctctccccgg gagtcgatgt cctcgagggg gagcgggtgg ggccggtcgt
     2701 cggtggtgat cttctcggcg gccgcgtggc ggtgcttcgc gacccactcg tcgtagtcgt
     2761 aggcgttgga gccgttcttg acgggcttgt cgaagtagtc ggtgtacatg ctcatgacgt
     2821 tgtcgccgac gacggggaag accggcaccc agtagttgac cagttgctgc gggtggccgt
     2881 accacatgtc gcggtgggcc ttgtagttgt aactgacccc ggcggtcagg aagttctcgg
     2941 ccgggataac tcgcagccgc gggatgtcga agtaggtgcg gtcgggatcg acgccgatgg
     3001 tgacggcgaa ctcttggcac aactccttcg tgcgctgtcc gttggtgaat tcggacttca
     3061 gcgggcccac ccgggcgatg aactcgtcga cggtcatttc gaactgggca cgctcggggt
     3121 cgaggtcacc gaaggtgtcg gcaatgaggc tctcggcgtg cttcaccaac gcggctgcac
     3181 cgggctgccc cgtgtagagg tagaaatcgc cggagaacag cgattcgcgt cggcggtcgt
     3241 ctgcgatctg acggttcagg tagagcgtga tcacgcaatt cctcgcttct ccggtcgatg
     3301 cacgtgcgac ttcgcgaagg gaggttaccg caccatcggg acggccgatc ctacgaagga
     3361 gcttagtagg acgtcccacg tggaatggta ggagtcagcc gggtgggcgg atgcggtcaa
     3421 acggggtcaa tccggagcac gcggtcgtcc gtaccgttgg acgtcgagac gaacagcgcg
     3481 ccgtcaggag cgacccgaac ggcacgcagc ctgccgaagg cgccgtcgag cggggcgggg
     3541 atggtgacgt tggccacggc gccgccgggg ccgagctgca tgagtaggag cttctcgccg
     3601 cgcagggcac ccaccgcgag cacaccgtcg aggtcgcccc actgccgccc ggagaggaac
     3661 gccgcgccgg acacggcctc cacggggttg cccgaactcc acgcggcggg caccgcgtcg
     3721 gggaagcgct ggaggtcggt catcggcacc gactcgtcgt acccgccgac ggtgcccccc
     3781 tgggacgggt cccatccgta gttggcgcct gcccccaaga ggttcacctc gtcgtcggtg
     3841 gtggggccgt gctcggcgga atatgccttg ccggtgtcag gtgtgaccgc gacgccctgg
     3901 acattgcggt gcccgtagct ccagatgagt cgctgcgcag ggttctgggc gtccgcgtac
     3961 gggttgccgg gagcgggccc gccggtgtcc aggtcgatgc ggagcacctt gccacccaga
     4021 gacgcgaggt cctgcgcgat ggcgccgcgc gcggtgtccc cggtgcccac gagcagggcg
     4081 ccgtcggcgg tgagggtcgg ccggcacccg gagtgccgtc ccgacgggtt gatcggcatg
     4141 ccgccgacca gcgggtccgc gaccctcgac gccgccgtgc cgtcggcgga gagctgccac
     4201 gtcaccagcc ggacgtccgt gggcgtgccg gccttcatgt gggtctggca cgtggtgaac
     4261 cggcgggtct gcgcgaagtc ggggtgtacg accatgccca tcagcccgcc ttccccgcgg
     4321 acgtacacgt ccgacaggtc cgcagtgact ggcgtcaccg tcgctccggg ctgcgtgctc
     4381 gacagcagcg cgatccgccc ggcgcgctcg gtcagcagta cccggccgtc gggcaggaag
     4441 ccgaggtccc agacgtgtga caggcccgcc gccaccacgc tgacccgaag ctccggcgtg
     4501 ccggtgcggg caggggccag cggggctgcc gccggcgtgg tgcttgcgct accccgctcg
     4561 atcgggctgg cctccggcgc ggtgcatccg gccagcccga gtgccgctgc gacggcggtc
     4621 gcgaccgcgt agcgggtggg cgtcattcgc tccgtcggct tggcctcgca ccgcagcatg
     4681 cgcccatcct gccgccgtcc gggtggaact cccgtatgca acacccgata tcggagaatc
     4741 ttgtcgatct cgggcgcctg ccgccccgga ctgatcgcta cggtctggcc atgccccgca
     4801 gttacctccg gtttccccac ctgcacgccg acacgctcgt cttcgtggcc gagaacgacg
     4861 tctggaccgc gccggtgtcg ggcgggaggg cctaccggct caccgccgac gacgtgcccg
     4921 tcgcccagcc acggctgtcc cctgacggcg cgcaggtcgc gtggacgtca tggcgtgagg
     4981 gcgctcccga ggtgttcgtc gccgatctcg acggcggggg tgcgcgccgg ctgtcctact
     5041 gggctgatcc ccgtacccag accatcgggt ggacgccggc gggggaggtg ctcgcggtca
     5101 gcgcagcggg gcaggcgtca ccgcggcggg ggtgggccta cgcgatcccg gcgacgggcg
     5161 gcaccccgcg ccgcctcgac ttcgggccgg tctccgatgt cgcgctgccc gcagggggcg
     5221 gtccggcggt ggtcgtcacc agcctcatga cgcgggatat ggcctggtgg aagcggtacc
     5281 ggggcggtac ggccggcaag ctctggtggg atgcggccgg gtcgggggag ttcgtccggc
     5341 tcgcggccga cctcgacggg cagctggccg cgccgatgct gatcggctcg ggcgacgggc
     5401 tgcgcatcgc cttcatctcc gaccacgagg gctggggcaa cctctactcg ctcggcgccg
     5461 acggtgccgg gttgcgccgc cacaccgacc acggcggccc ggacgcgccg gcgttctacg
     5521 tgcggcacgc cagcaccgac ggcgagcgga tcgtgtacga gtcggcaggc gagctctgga
     5581 tcctcgactc gctcgacagc gccgcccccc ggcggctcga cgtgcggctc ggcggcccgc
     5641 gcaccgcgcg cgacccgttc cgcgtccgca cggccgacga actcagctgg gtcgcacccg
     5701 accgcaccgg gcgcacgagc atcgcgcagg tgcgcggcac tgtccaccgg ctcactcacc
     5761 gcgacggccc cgcccgcacg ctgctcgccg agccgggggc ccgggcccgg ttggcccgcc
     5821 cgctgggcga cgaccgtgcg gtgtgggtgg acgacgtgga gggggaggac gcgatctgca
     5881 tcgccccgct cgacgagcgg gccgacggcg ggcagccgct tcgccgctac ggagccggcg
     5941 cggtgggccg cgtgctggag ctggccgccg ccccggacgg gtcgaagctg gcgctggccg
     6001 cgcacgacgg gcgactgctg gtgctggagc tcgccggttc ccgtgcgggg gagctgcgcg
     6061 agctggcccg gggcgccgac ggcgagatct cggacctctg ctggtcgccc gacagtgcct
     6121 ggctggccta cagcgacccc gtcgaggccg gtctcagtcg gatcatgatg gcccggctcg
     6181 ccgacgacac gctggtggcc gtcaccgagc cgcgcttcgc cgacaccgat cccacgttca
     6241 ccttcgacgg caagtacctg gccttcctgt cccggcgcag cttcgacccc atctacgacc
     6301 agcactcctt cgacctcacc ttcccggcgt cgtggcggcc gttcctggtg ccgctggccg
     6361 ctcgcacgcc ttcgccgttc ggcgcgagtc cggacgggag gcccgtgtcg gcgcaggacg
     6421 agggcccgga ggacccgccc gctcccgacc cgtcggaccc gtcggccgag ccgacacccg
     6481 acgccggcgg cgagggcacg ggcaaaccgg agaagaagaa gggtgaccct ccgcccgagg
     6541 tcgtggtcga cgtcgagggc ctggccgccc gggtggtgcc ggtgccggtc gtcgcggccc
     6601 gctacagcgg catgcaggcc ggaaaggact gcctgctgtg gtaccgcagc ccggtctccg
     6661 gggtgctggg ggacacccgc gccggcaccg acgagaaggc ggagcggccg gtgctggagc
     6721 gctacgacct ggtgcgccgc aagctcgacg tgatcgccga cccggtcagc tcgttcgccg
     6781 tgagcggtga cggctcccgg ctcgtcgtgc gcgacggcgg agcgctgcgg gtgctgcgca
     6841 ccgaccggtc cggctccgga gcgccggggg agggcgacgg tgacgagttc gagatcgaca
     6901 cccgccggat cgtcgtcacg gtcgacccca ccgccgaatg gcgccagatg ttcgacgaag
     6961 ccggcaggct gatgcgcgac cacttctgga cggaggacat ggccggcgtg gactgggccg
     7021 cggagctggc gcgctaccgg ccgctcgtcg acgccgtggg cagccacgac gacctcgtcg
     7081 acctgatgtg ggagctgcag ggcgagctcg gtacctcgca cgcgtacgtg atgggccacg
     7141 gggcaggtga ccacagcggg cagccggggt tgctgggcgc cgatctcgag ccaggggagc
     7201 cggacggatg gcgggtggtg cgcgtgctgc ccccggagac gtctgcaccc gcggccagga
     7261 gcccgctgtc cgggccgggc gtcgacgtcc gggcaggcga tctgatcctg gaggtcggtg
     7321 gcaagccggt cgaccccgcc tacggcccgg ctccgctcct ggtgtcgcgg gcgggggagc
     7381 tcgtggagct caccgtgcgc tccggacccg atcgggagga cgcaggcgag gtacgccggg
     7441 tagtggtgcg gccgctgggg tccgagacct cgctgcgtta ccaggactgg gtggccggca
     7501 gacgtgcctt cgtggccgac cggtccgagg gcaggctcgg ctacctgcac gtgcccgaca
     7561 tgatggcgcc gggctgggcg cagttgcacc gcgacctgac ccgggagacg agccgcgagg
     7621 ggctgatcct cgacgtccgc ggcaacagcg gtggtcacac gtcacagctg gtggtggaga
     7681 agctggcgcg caaggtgatc ggctgggacc tgccgcggca ccggcagccc accacctacc
     7741 cggaggacgc gccgcgcggc cccgtcgtcg cgctcgccga cgagctgtcg ggctccgacg
     7801 gcgacatcgt cacggcggcg atcaaacggc tgggtatcgg tccggtggtc ggcgtccgca
     7861 cctggggcgg ggtggtcggg atcgacagcc gctacggcct cgtcgacggc acgcgcgtga
     7921 ctcaaccgcg gtacgcgttc tggttcgacg acgcgcactg gggcgtcgag aaccacggcg
     7981 tcgaccccga cgtcgaggtg gtgatcgccc cgcaggactg ggtggcgggg cgcgaccccc
     8041 agctggagcg ggccgtcgac atggcactcg aggcgctggc cgagcggccg gccgtgcggg
     8101 cgccggacgt ggccagccgc ccgagccgac gtcgcccgga cctcccgccg cgcccctgac
     8161 ggcgatggtg ctgccctccc gggtgaacgg cactcccgcc cgatccgtcc ggacgggagt
     8221 gccgtgcgtg cggggaggcc tcagccctgg ttggtcacgg ggccggcttc ttccgggccg
     8281 ccggcaggtg ccccgagcgc accaggcagc cgagggtgtc gcagatgacc cgggtggcga
     8341 tcagggcggt gatctccgcg ttgtcgtagg gcggggagca ctccaccacc tcgatgccgg
     8401 cgagcgggcg cgcgtcggcg atgatctgga tgaacttcag gacctccctc ggcaggaacc
     8461 cgccgggctc gggccagccg gtgcccggca cgaacgctgc gtcgaggcag tcgacatcga
     8521 acgacagcca gacggcgtcg gcgccgtccc acgccacgtc gagcgcgcgc tgtgcggcgt
     8581 cctcgatgcc catctcgacg cagtcggtga cggtcatgat cgtggtgccg cgctcgcggc
     8641 cgaccttcac accgggccgc ggggcctgcc agccgccgat gccgatctgc accaggttct
     8701 tggcggggac gttcgggagg tccgtggcat ggaaccaggg cgtggtgtgc atccgctcgt
     8761 ccaggtcggt ctcctgggtg tcgacgtggc gatcgaaatg gatgatcccg aggttgccgc
     8821 cgttcaggtg cggggcgacg ccgcgggtgg tggggtagcc gatcgagtgg tcaccgccga
     8881 gcaccacggg gaacgcgccg ctctcgtaca cgtgcgacac ggccttggtg atctggtcga
     8941 aggtcttctc gatgttgccc gggatcgtga agatgtcgcc gagatcgccg atggtgatcg
     9001 actcccgcag gtcgacaccc agctcgaagc tgtacggccc gtagagcgcg gagatcttcc
     9061 ggatgccctg cggcccgaag cgggtgccgg aacggtaggt cgtgccgccg tcgaacggcg
     9121 ccccgagcac ggccacgtcg tactcgccgc agcggcgcac gtcctccacg tagggcgcct
     9181 tcagaaaggt gttgatgccg gcgaagtgcg gcagctctcc ccgggagaag gtcgggatcc
     9241 tccggtcgac gatcgagtcg gcgccctgca ggcccagctc gagcccgcgc gccacctcct
     9301 cctcccactt cgtcgtcggc aggccggcct cggcccggac ggcgaacgcc gcctcggggc
     9361 cgtagttgtc gtggatctcg ccgtcgccgg tcctgtcggg ctgctggggg tggtagtgca
     9421 cgtgcactcc tcacggtcgg gggcgccgga gcggagcagc cggcgcccac cagctgtctc
     9481 ccgggctttt gtcccgccgt ggggtggccg cacgtggcgc ggccgcctgc atctctcgga
     9541 ccagcccccc gtcaccgggg gcggaaccct agacgcgcct cactcgtgtg agtcaccacg
     9601 agcctagaca cgctttgttg ccgccgcgcg cagctgccgt taccccgccg tgaaaggcgc
     9661 cgggccgctg ctctcaccgt cgggacgtgg gcgcgagatc caggccagtg cctggcgagc
     9721 gtaggctggc ggacacgacc ccaccgggcg ttcctcccgg gtaggggtct gtcctgtgtg
     9781 cgctgcccat gctctgcgag gggtccccga caccgtggcc aagctctccg gtctgctccg
     9841 ttccagcctg tccgatcccg acctgcgggg cgtcgtcgag gccgctcgcg acacccgtgc
     9901 cgacgaggtg cctgctctgc ggatcgaggg cccgggagcg ctgcgcccgt tcctcgctgc
     9961 ggcgttcggc accgccgccg acgcgggcgg cgcaggccac acggtgctcg ccgtcaccgc
    10021 caccgaccgg gaggccgagg acctcggcgc cgcggcaggc gacctgctcg gccgggccgc
    10081 agtcgccgtc ctgccctcgt gggagacgct cccgcacgag cggctctcgc cccgccccga
    10141 caccgtgggg cgcaggctcg agatcttccg gcggctggcg aatcccgaga cggcgccgcg
    10201 cgtggtggtc gccgccgcgc gcagcctcat ccagccgatc gcgccggggc tgggtcggct
    10261 cgaccccgtc cagctgcggg tgggcgacat cgccgacttc gacgcgctgc tcgtacggct
    10321 cgtcgagttg gcctacacgc gcgtcgagat ggtcaccgcc cggggcgagt tcgcggtgcg
    10381 cggcgggatc atcgacgtgt tccccccgac ggccgagcac cccgtccgcg tcgagctctg
    10441 gggcgacgag gtcagcgagc tgcggtactt cgccgttgcc gaccagcgct ccgtcgggcc
    10501 cgtcgaggag ctggtcgcgc cggggtgccg cgagctgctg ctcaccgagc cggtgcggga
    10561 gcgagccgcg gcgctcgccc gcgcccacga gaacaaccct ccgctgcgcg agctcctcga
    10621 gcacctcgcc gagggcatcc cgtcggaggg catggagtcg ctggtaccgg cgctggtcgg
    10681 gggcgagctg gagctgctca ccgacctgct gccggcggac gggctcgtgc tgctcgccga
    10741 cccggagcgg atccgcaccc gcagcgccga cctggtgcgc accgggcagg agttcctgga
    10801 ggcgtcgtgg tttgccgcgg gtaccggtgg cgcggcaccg atcgacgtcg gggcgtccgc
    10861 ctaccgcgac ctcggcgagg tgctcgacca cgcctcggcc accggacggc cggtggtcag
    10921 cctgagcccg ctgttgtccg gtggtgacga cgccctcgcc ccgaccgtgc acgaggtcga
    10981 gagctaccgc ggcgacaccg accgcgcact cgtcgacctg cgtgcgcacg tcgccaccgg
    11041 cggcacagcc gtcctcgtcg tcgccggtca gggcaccgcc cagcgctcac tggagcagct
    11101 gcgtgaggcg gacgtgccgg ccaagctggt cgaggagctc gccgacatcc cggaaccggg
    11161 gctggtcacc gtcacctgcg ggcgactcgc cgagggcttc accgcgcacg ggctcgtcgt
    11221 cctgaccgag gccgacctca ccggcaaccg ggccgcgggc accgagccgc gcaagctcgc
    11281 gtccaagcga cgcaacgccg tcgacctggt gacgctgcag cccggcgact acgtcgtgca
    11341 ctcccagcac ggcatcggca agttcgtcga gatgcgcgag cgcaccgtgg gcggtgccac
    11401 ccgcgagtac ctcgtgctgg agtacgcctc gtccaagcgg ggccagcctg ccgaccgcct
    11461 cttcgtcccc acggactcgc tcgacgagat cagccgctac gtcggcggcg aggtacccac
    11521 gctcaacaag ctcggcggcg gtgactgggc caagacgaag ggccgcgccc gcaaggcggt
    11581 ccgccagatc gcggcgcagc tcgtgcagct ctacgccgcg cgccagtccg cgcccggcca
    11641 cgcgttcgcc aaggacacgc cctggcagcg tgagctcgag gacgcgttcc cgttcaccga
    11701 gacgcccgac cagctcgccg cgatcgagga ggtcaagcgg gacatggagc agccggtccc
    11761 gatggaccgg gtgatctccg gcgacgtggg cttcggaaag accgagatcg cggtccgcgc
    11821 tgcgttcaag gcggtgcagg acggcaagca ggtggcggtg ctcgtaccca ccacgctgct
    11881 cgccacccag cacctgcaga cgttctccga ccggatgcgg gccttccccg tgacggtcaa
    11941 ggggctctcc cggttcaccg acaacgccga ggcgaagcag accatcgagg ggctggccga
    12001 cggcaccgtc gacatcgtcg tcggcacgca tcgcctgctg cagaccggca tccggtggaa
    12061 ggacctcggc ctcgtgatcg tcgacgagga gcagcgcttc ggtgtggagc acaaggagca
    12121 catcacggcg ctgcgcaccc atgtcgacgt cctcacgctg agtgcgacgc cgatcccccg
    12181 gacgctggag atgagcctcg cgggcatccg cgagatgtcg acgatcgcca ccccacccga
    12241 ggaccggcat ccgacgctca cctacgtcgg cgcctacgac gagaagcagg tgggcgccgc
    12301 gatccggcgc gagctgctgc gcgacggcca ggtgttctac gtgcacaacc gcgtgtcgac
    12361 gatcgaccgc gcggcgcgga ggatccgcga gctcgtgccc gaggcgcggg tggcgatcgc
    12421 gcacgggcag atgaacgagg acctgctgga acgcacggtc aacgggttct ggcacaacga
    12481 gttcgacgtc ctggtctgca ccacgatcgt ggagaacggc ctggacatct ccaacgccaa
    12541 caccctgatc gtcgagcgtt ctgacacgct cgggctttcg cagctgcacc aactacgcgg
    12601 ccgggtgggc cgcgggcgcg aacgcggcta cgcgtacttc ctgttcccgc cggagcaccc
    12661 gctcaccgag accgcccacg accggctcgc gacgatcgcc cagcacagcg aactcggcgc
    12721 gggcacggcc gtggcgatga aggacctgga gatccggggc gctggcaaca tcctgggcgc
    12781 ggagcagtcc gggcacatcg ccggcgtcgg gttcgacctc tacatccggt tggtcggcga
    12841 ggccgttgcc gcgttccgcc agcaggcggg tgcaggggat ggcgaggacg tcgagcagct
    12901 cgccgacgtg cgggtggacc tgccggtcga cgcgcacgtc ccgcacgact acgtcacggg
    12961 ggagcggctg cggctggagg tctaccgcaa gatcgccgaa gcaggcgagg ccgccgacct
    13021 ggatgccatc gtcgaggaga tcaccgaccg ctacggggag ccgcccacgc cggtgcgcaa
    13081 cctcctcgcc gtcgccgcgt tccgccagac ctgccgggcc ctcggcgtgg cagaggtggc
    13141 agtggcgggc aacggcgtcc ggatcgggcc cgtagacctg cccgactcgg ccacgctgcg
    13201 gctgaagcgg ctgtatccga aggccgtcta ccggccggct gcgcgggccg tcctcgtgcc
    13261 gaagccggtc gagagcggcc gcatcggcgg cgcaccgctg cgcgacgtcg agctgttgga
    13321 ctggtgcacg aagctgctgc gcgacctcct ggccccggcg aaggtgcccg cctccgtctg
    13381 atcagcgggg acggctatag gtcgggtacc gctccccgct cgggcgggat cggccggcgc
    13441 atccgggcag gccgtcggta acgctgctcg gcctccggga gagcttccgg caggagccct
    13501 cccgggaacg gcccggggcg gggcatgacc cccggagcgt cgtttgttcg ttcatcagga
    13561 gcccgagccg gcatcccgcg gaaggcccct gcaaccgcct ctcgtgtacg gacccgagct
    13621 cgcgacctat ggccagcggg tcggcggttt cctcatcgac gtcggcatcc cgaccgggct
    13681 gctggtcgcc gtcctgatcg ccgcgctggc cacccgcgat ctcacgatga tcatggtggt
    13741 ctacggcctc tcggcggcga tgttgaccgc cttcgtgctg tggaacagcg ggtaccgcca
    13801 gggcaggtcc gggcagagca tcggcaagag ggcgctgggc gtgcggctcg tcacggcgga
    13861 gaccgggcaa ccggtcgggt tcggccgcgc cgtcggccgg cagttcgccc acctgctcga
    13921 cgggatcccg ttctccctcg gctacctgtg gccgctgtgg gacgagcaac ggcagacctt
    13981 cgcggacaag gtgtgctgca cgctggtggt gcaggccgac atgtgaaccg cggcacggag
    14041 ccgacgcacg ggcgccggtg gacgggccgg gagcgcgtga gagggtggtg gccgtgcgca
    14101 agcaggtggg gcgcgtcgca gcggccgtag cagtcgtcgg cgcgatggtc agcgggtgtg
    14161 gtctggatca ggcggggtcg gcggtgatcg tcggcaacga tgccgtgcca ctcgggcgcg
    14221 tccagtcgca gctcgacgtg gcgctgggca agaccgacca gatcgcccag ctcactgcgc
    14281 agggcggaac gccagccgac ctctcccgca gcatcgtcac ccgcgaggtg ctgcacgacc
    14341 tgctccagcg acgggccgcc gaggacggca tcgtcgtgac cgacgcgcag gtcgaggccg
    14401 agctggcggg cagcggcggc gccgacgcgg tcgtcaacg
//
LOCUS       DASRSC010000375         6467 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_1473027,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000375 DASRSC010000000
VERSION     DASRSC010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 6467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6467
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473027"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..178)
                     /locus_tag="VFE39_18210"
     CDS             complement(<1..178)
                     /locus_tag="VFE39_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET6260576.1"
                     /translation="MQPRATRHLFVTGGVASSLGKGLTASSLGQLLTSRGLRVIMQKL
                     DPYLNVDPGTMNPFQ"
     gene            488..1525
                     /locus_tag="VFE39_18215"
     CDS             488..1525
                     /locus_tag="VFE39_18215"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HET6260577.1"
                     /translation="MESMERALAERVVHGLDDTLDHVSLRRQASSRLRGQVAFDLAMW
                     AVLDPATIMWGSCVVDNAPRDFDDLEDAVFENEYRARDVLKLVDLAAGPGIGTLCGVT
                     GGDPAISPRYRQILAPRGFTDELRLVMHDGRAAWGLLCLLRAGGSFDDGEVDALAGLG
                     QPFATALRRSLVRGAADVTGPAPAGLILATTTGRILDMAEDARRLLDATPVDDLPLVI
                     QAVAARRAVGRPASAACPTRDGRWLAFHASALGDRLAVVVEVVRPHQLADLVVRSRGL
                     TPCEQEVLAAVARGRPDRMIARELMLPEHAMQDHLRSVFAKFDVGSRTELVAALFFEH
                     FAPLHAADSTV"
     gene            complement(1551..2471)
                     /locus_tag="VFE39_18220"
     CDS             complement(1551..2471)
                     /locus_tag="VFE39_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010697200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HET6260578.1"
                     /translation="MISLRYHAVSIAAVFLALAIGVVLGASGVSDRLLSAVSAKADDL
                     GGQVQTLTAQRDALAAGQRASDEFAQRIGPAAVRGLLQGRTVALVTSGAEPADRDAVL
                     ALLGPAGATVSGEIALTQAVGDPARADQLRELTSQLLPTGAQLPAASDTGSLVGGLLA
                     GVLVASSGQVAPGQADALLAGLSAAGFVAPGDRPRPSDLVLVLTGGAIPGVDGGDAAA
                     VIARLAAQLDRAGGGAVLAGRAGSADATGAVGVARADPNITASLSTVDDVQTGPGRVS
                     AVLALREQLDGRAGRYGTAVTAADGATPAA"
     gene            complement(2468..3652)
                     /gene="steA"
                     /locus_tag="VFE39_18225"
     CDS             complement(2468..3652)
                     /gene="steA"
                     /locus_tag="VFE39_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HET6260579.1"
                     /translation="MRLSGLRHRSRPELPGLSGVARVDRRTDGLLRRVKQGEIAVLDQ
                     VDLDRATADALVAAEVAAVVNVSPSISGRFPNLGPEVLIDAGITLVDSVGAEAMHAIK
                     DGSRIRLHDGVVYAGELRLADGVEQTADAVADAMVEAKSGLTHQLEAFAANTIEFMRR
                     ERAMLLDGAGVPDVEVELAERQVLVVAAGFDHATELARLKHYIREYKPVLVGVGAGAD
                     ALLAAGYRPQLIVGDPGEVSTEALTCGADVVVPAFSDGHAPGLHRVQDLGAGAVTFPS
                     SANPEDLALLIAAHHGASLVVTVGLSASMAEFLDRGRSGSIASTFLTRLQLGGTLVDG
                     RVIATLYRSRISFGAILLMVAAALVAVVVALLVSDAGDAVLVWLAHGWSGVAEQVTGW
                     FG"
     gene            complement(3789..5600)
                     /gene="recN"
                     /locus_tag="VFE39_18230"
     CDS             complement(3789..5600)
                     /gene="recN"
                     /locus_tag="VFE39_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET6260580.1"
                     /translation="MLAEMRIQGLGVIDDATLELDPGLTVLTGETGAGKTMVVTGLSL
                     LGGGRAETSRVAEGARRAVVEGRFSADPAALAVADEVGADADEDGTLIAVRTVGADGR
                     SRAHLGGRSVPVGVLSRLAEVTLAVHGQNDQLRLLRPAEQRALLDRFAGEAVGVPLER
                     YRTVRAEWQRVAAELVERRDGARRLAQEADLLRHGLTEITAVDPQPGEDVELVELAHR
                     LSAADDLREVASGAQFALVGAEDGGDPDAPGALALIGDARHRLAAAGDPHLTALDARL
                     EEVLALLGDVGAELTGYLDRLDADPERLAVVLSRQAELKALTRKYAADIDGVIAWADA
                     ARERLAGLDTSDEALSALAARRDALGLELAEHAAAVTAARAEAAARLAADTTGELGGL
                     AMPDARLLVAVVPREAGPGAADALRVGPTRLVAGTDGVDEVELRLVAHTGAAPLPLHK
                     GASGGELSRVMLALEVALAGADPVPTMVFDEVDAGVGGRAAVEIGRRLARLAARHQVI
                     VVTHLPQVAAYADRHLVVDKTAGNDAAPTRSRVRTLAEGDRIVELARMLAGLDDTDTG
                     RAHAEELLGAARAHREADRAGTNGAVRKSRRKTAARV"
     gene            complement(5635..>6467)
                     /locus_tag="VFE39_18235"
     CDS             complement(5635..>6467)
                     /locus_tag="VFE39_18235"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013675907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET6260581.1"
                     /translation="ASGVRLRVIGDEWAEVENGDLPASLEPRVVEGRPGCAEGAEVVL
                     VLGGDGTLLRAAETARPVGVPLLGVNLGRVGFLAEAEEESLDHALEAIVEGSYSVEER
                     MTLDAVARTNGHVLARTWALNEATVEKVSRERILEVVLEVDGRPVSAFGCDGVICATP
                     TGSTAYAFSAGGPLVWPQVKALLLVPSNAHALFARPMVIAPDSEVAIEVDQSGPPAVL
                     ECDGRRTVALPPGARVELAAGATPVRMVRLDGQPFADRLVRKFNLPVRGWRGAPQSAS
                     "
BASE COUNT          883 a         2494 c         2279 g          811 t
ORIGIN      
        1 gctggaacgg gttcatcgtg cccggatcca cgttcaggta cgggtccagc ttctgcatga
       61 tcacccgcag accgcgtgag gtgagaagtt gcccgaggct cgaggccgtc agacctttac
      121 ccagcgagga cgcgaccccg ccggtgacga acagatgacg cgtggcgcga ggctgcacgg
      181 ggtttcaggg tacataggac agcgggcacc ggcgccccta cccgcgtgtc gaggacaaga
      241 tgcctgatcg ggcgcggtcc gttcaggtgg atccgaacac tgacggtgag accgcggtgt
      301 cggcccgtgg cccccaccgg gcggacgacc ccgaccgggg gagacccgag atcccggcca
      361 tacctcacgg tattccgcct ggatgttgcg ttcatggccg gtgaggcgag tcaacccttg
      421 ggaacctcgc ggtccgcggc ccatccctcg tccctggggg atgtggcggg cccgggacgg
      481 agccggaatg gaaagcatgg aacgggcgct ggccgagcgc gtggtgcatg gcctcgacga
      541 caccctcgac cacgtgtcgc tacggcggca ggcgagcagc cgcctccgcg ggcaagtcgc
      601 gttcgacctg gccatgtggg cggtcctgga tccggcgacg atcatgtggg gatcctgcgt
      661 ggtcgacaac gcaccccgcg acttcgacga cctcgaggac gcggtcttcg agaacgagta
      721 ccgggcgcgc gacgtgctga agctggtcga cctcgccgcc gggcccggca tcgggacgct
      781 ctgcggggtc accgggggcg accctgccat cagcccgcgc tatcgccaga tcctcgctcc
      841 ccgcgggttc accgacgaac tgcgcctggt gatgcacgac ggccgggccg cgtggggcct
      901 gctgtgcctg ctgcgggcgg gcggttcgtt cgacgacggg gaggtcgacg cgctggccgg
      961 cctgggacag ccgttcgcca ctgcgctgcg cagatcgctg gtccgcggcg ctgccgacgt
     1021 caccggtccg gccccggccg ggctcatcct ggccacgacc accggtcgca ttctcgacat
     1081 ggctgaggac gcacgccggc tgctcgacgc caccccggtc gacgacctgc cgctggtgat
     1141 ccaggccgtc gcggcccggc gcgccgtagg gcgcccggcc agcgccgcgt gcccgacccg
     1201 cgacgggcgc tggctggcct tccacgcgtc cgcgctgggc gatcgcctgg cggtggtggt
     1261 ggaggtggtc cggccgcacc agctggccga cctggtcgtg cggagcaggg ggctcacccc
     1321 ctgcgagcag gaggtgctgg ccgccgtcgc ccgcggccgg cccgaccgga tgatcgcccg
     1381 cgagctcatg ctgcccgagc acgcgatgca ggaccatctg aggtccgtgt tcgcgaagtt
     1441 cgacgtcggg agccgcaccg agctggtcgc ggccctgttc ttcgagcact tcgccccgct
     1501 gcacgccgcg gacagcaccg tctgagcgag caggcgtgac ctgcgcggcg tcacgcggcc
     1561 ggggtggctc cgtccgcggc cgtcaccgcg gtgccgtagc gcccggcacg cccgtcgagc
     1621 tgctcacgca gcgccagcac cgccgacacc cggccgggcc ccgtttgcac gtcgtcgacc
     1681 gtggagagcg acgcagtgat gttcgggtcg gcccgcgcca cccccaccgc gcccgtcgca
     1741 tccgccgaac ccgcgcgccc ggcgagcaca gccccgccgc ccgcgcggtc cagctgggcg
     1801 gccagccgcg cgatgacggc cgcggcgtcg cccccgtcga cgccggggat cgccccgccc
     1861 gtgagcacga gcacgaggtc cgacggccgt ggccggtcac cgggtgcgac gaacccggcc
     1921 gccgagagcc cggcgagcag cgcgtcggcc tgcccggggg cgacctgccc ggacgacgcc
     1981 accaggacgc cggcgagcag cccgcccacc aggctgccgg tgtcggaagc cgcgggcagc
     2041 tgcgcgccgg tcggcagcag ctgtgaggtg agctcgcgga gctggtcggc gcgagcgggg
     2101 tcgccgacgg cctgcgtgag tgcgatctcg ccactcactg tggcgccggc cgggccgagc
     2161 agggcgagta ccgcgtcccg gtcggcgggc tccgcgcccg acgtcaccag cgcgacggtg
     2221 cggccctgca gcagcccgcg gaccgcggca ggcccgatcc gttgtgcgaa ctcatcggac
     2281 gcgcgctgac cggcggcgag ggcgtcgcgc tgggcggtca gcgtctgaac ctgcccgccg
     2341 aggtcgtcgg ccttggcgga gaccgccgag agcagccggt cggacacgcc gctcgcaccc
     2401 agtaccacgc cgatcgcgag cgcgaggaac actgcggcga tcgacaccgc gtggtaccgc
     2461 agcgagatca tccgaaccac cccgtgacct gctcggcgac ccccgaccaa ccatgggcga
     2521 gccagaccag cacggcgtca cccgcgtccg acacgagcag cgcgaccacc accgcaacca
     2581 gcgctgcggc gaccatcagg aggatcgcac cgaacgagat gcggctgcgg tacagcgtgg
     2641 cgatcacccg gccgtcgacg agcgtgccgc cgagctgcaa ccgggtgagg aacgtcgacg
     2701 cgatgcttcc cgagcgtcca cggtcgagga actcggccat gctcgccgac agcccgacgg
     2761 tgaccacgag cgacgcgccg tggtgcgccg cgatcagcag ggcgaggtcc tcggggttgg
     2821 cggagctcgg gaacgtcacc gcgcccgccc cgaggtcctg aacgcggtgc aggccagggg
     2881 cgtgcccgtc ggagaaggcc gggacgacga cgtcggcgcc gcaggtcagc gcctcggtgg
     2941 acacctcccc tggatcgccg acgatgagct gcggccggta cccggccgca agcagcgcgt
     3001 ctgcgccggc gcccacgccc acgagcaccg gcttgtactc acggatgtag tgcttcagcc
     3061 gagcgagctc ggtcgcgtgg tcgaaccccg ccgcgacgac gagcacctgc cgctcggcca
     3121 gctcgacctc gacgtcgggc acgcctgcgc cgtccagcag catggcgcgc tcgcgccgca
     3181 tgaactcgat ggtgttggcc gcgaacgcct cgagctggtg cgtgagaccg gacttggcct
     3241 ccaccatggc gtcagccacg gcatccgcgg tctgctcgac gccgtccgcc agccgcagct
     3301 ccccggcgta gaccacgccg tcgtgcagcc ggatccggct cccgtccttg atcgcgtgca
     3361 tcgcctcggc gccgacgctg tcgaccagtg tgatccccgc atcgatcagc acctccggcc
     3421 cgaggttggg aaaccgcccg gagatcgagg gggacacgtt gacgacggcg gccacctcgg
     3481 ccgcaacgag ggcgtccgcg gtggcgcggt cgaggtcaac ctggtcgagc accgcgatct
     3541 cgccctgctt gacgcggcgg agcagtccgt cggtgcgccg gtcgacacgc gcgacgccgg
     3601 acagcccggg cagctccggc cgggagcggt gccgcagacc ggataacctc atttcacgca
     3661 cccgccttcc atgaacgcac tgtggcatct ggggcatgtg ataccgggaa ggcacgccgc
     3721 gcgatcaccc gagcgttcac ccccggcgac ccgcgaagcc ctaccggacg ccggcacggg
     3781 tggccgtgtc agacgcgggc agctgtcttc ctgcggctct tgcgcaccgc gccgttcgtg
     3841 cccgcccggt cggcctcgcg gtgggcccgg gcggcaccca gcaactcctc ggcgtgggcg
     3901 cggccggtgt cggtgtcgtc cagcccggcc agcatccgcg ccagttcgac gatgcggtct
     3961 ccctcggcca gcgtgcgcac ccggctgcgg gtgggggccg catcgttccc cgccgtcttg
     4021 tccaccacga ggtgccggtc ggcgtaggcc gcgacctgcg gcaggtgcgt caccacgatc
     4081 acctggtggc gcgccgccag gcgggcgagc ctgcgcccga tctccaccgc ggcccggcct
     4141 ccgacgccgg catcgacctc gtcgaacacc atggtgggga cggggtcggc cccggcgagc
     4201 gccacctcca gcgcgagcat gacgcgcgac agctcaccgc cggacgcgcc cttgtgcagc
     4261 ggcagcggtg cggcgcccgt gtgtgcgacc agccgcagct ccacctcgtc caccccgtcg
     4321 gtgccggcca ccagcctggt gggcccgacc cggagcgcgt cggcggcgcc cggcccggct
     4381 tcgcgtggga cgactgccac gaggagccgc gcgtcgggca tggccagccc gcccagctcc
     4441 cccgtggtgt cggccgccag ccgcgcagcc gcctcggcgc gggccgccgt gaccgccgca
     4501 gcgtgctcgg ccagctcgag gcccagcgca tcccggcggg ccgcgagtgc gctcagtgct
     4561 tcgtcagacg tgtcgagacc ggccagtcgc tcccgggccg cgtcggccca ggcgatcacg
     4621 ccgtcgatgt cggccgcgta cttacgggtg agtgccttga gctcggcctg ccgcgatagc
     4681 accaccgcca gtcgttccgg atcggcgtcg agccggtcga gatagcccgt gagctccgcg
     4741 ccgacgtcgc cgagcagcgc gagcacctcc tccaaccgcg cgtccagcgc ggtgaggtgg
     4801 gggtcaccgg cggcggcgag gcggtggcgc gcgtcgccga tgagcgcgag cgcacccggc
     4861 gcgtcggggt cgccgccgtc ctcggccccc accagggcga actgggctcc cgaggcgacc
     4921 tcgcgcaggt cgtcggcggc ggacagccga tgggccagtt cgacgagctc cacgtcctcg
     4981 cccggttgtg ggtccaccgc agtgatctcc gtgaggccgt ggcgcagcag gtcggcctcc
     5041 tgcgccagcc gccgcgcccc gtcacggcgc tccacgagct cggcggccac ccgctgccac
     5101 tcggcgcgga ccgtcctgta gcgctccaac ggcaccccga ccgcctcacc ggcgaaccgg
     5161 tccagcagcg cccgctgctc cgcggggcgc aggagccgca gctggtcgtt ctgcccatgg
     5221 acggcgagcg tgacctctgc caaccgggac agcaccccga ccggcaccga ccgcccaccc
     5281 aggtgcgcgc gggaccggcc gtcggcaccc acggtgcgaa ccgcgatcag cgtgccgtcc
     5341 tcgtcggcgt cggctcccac ctcgtcggcc accgccagcg ccgccgggtc ggcgctgaac
     5401 cggccctcga ccacggctcg ccgcgcccct tcggccacgc gcgaggtctc cgcccgcccg
     5461 cctccgagca gcgacagccc ggtgacgacc atcgtcttgc ccgcaccggt ctcaccagtg
     5521 agcactgtga gcccgggatc cagttcgagc gtcgcatcgt cgatcacgcc gaggccctgg
     5581 atccgcatct cggccagcat ggggcgaagc gtacggcgcc gacccgacat cgcttcagct
     5641 ggcggactgc ggcgcgccgc gccatccccg gacgggcagg ttgaacttgc gcaccagccg
     5701 gtcggcgaag ggctgtccgt ccagccgaac catccgcacc ggagtggcgc ccgcagccaa
     5761 ctcgacgcgc gctccgggcg gcagggccac cgtgcgccgc ccgtcgcatt cgaggacggc
     5821 aggcggaccg gactgatcaa cttcgatcgc cacctcgctg tccggggcga tcaccatcgg
     5881 cctggcgaac agcgcatgcg cattgctcgg taccagcagc agcgccttga cctgaggcca
     5941 gaccagggga ccgcctgcgg agaacgcgta cgccgtggac cccgtcgggg tcgcgcagat
     6001 cacgccgtcg cacccgaacg ccgacaccgg ccgcccgtcc acctcgagaa ccacctcgag
     6061 gatccgctcc ctgctgacct tctcgacggt cgcctcgttg agcgcccacg tccgggccag
     6121 cacatgcccg ttggtgcggg cgacggcgtc gagcgtcatg cgttcctcga ccgagtagct
     6181 gccctccacg atggcctcga gggcatggtc gagcgactcc tcctcggcct cggccaggaa
     6241 cccgacccgc ccgaggttca ctccgagcag cggcaccccc accggacgag ccgtctctgc
     6301 ggcccgcagg agcgtcccgt ccccgcccag cacgagcacc acctcggcgc cctcggcgca
     6361 cccgggccgc ccttcgacga cacgcggctc gagcgaggcc ggcaggtcgc cgttctccac
     6421 ctcggcccac tcgtcgccga tcacccggag ccggacaccg gacgcgg
//
LOCUS       DASRSC010000376        10672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_4746636,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000376 DASRSC010000000
VERSION     DASRSC010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10672
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4746636"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VFE39_18240"
     CDS             complement(<1..312)
                     /locus_tag="VFE39_18240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260582.1"
                     /translation="MQQPPQWEPQSRVQEQPAWEPQSRVQEPPAWEPRAREPQPWEAQ
                     PRERLRAPEPRAPEPLSREPLSREHLSREPLSAWESQSREPQVREPLLREPQPRDAQS
                     RD"
     gene            complement(693..1703)
                     /locus_tag="VFE39_18245"
     CDS             complement(693..1703)
                     /locus_tag="VFE39_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HET6260583.1"
                     /translation="MRLGVLDVGSNTVHLLVVDAHRGGHPTPMTSDKTELRLAENLDV
                     SGALTKAGADALVRAVQRASVAAAAAGCADLLAFATSALRDAANSAAVLAKVRDQTGV
                     ALQVLPGEDEARYTFLAVRRWYGWSAGRLLVLDIGGGSLEIAAGLDEHPAVAESLPMG
                     AGRLTREWFRSDPPSRREIAALRDHLDAQLAPVAHRLRRAGEPELAVGTSKTFRSLAR
                     LTGAAPSSAGPRVHRSLTDVGLRQLAAFITRMSSGDLAELEGVSPSRSHQLVAGALVA
                     EASMRALRVSQLEICPWALREGVILRRLDTLDGTVQTDRSAQDAPSVLGRLTSYEARC
                     TG"
     gene            complement(2235..2924)
                     /locus_tag="VFE39_18250"
     CDS             complement(2235..2924)
                     /locus_tag="VFE39_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013222396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET6260584.1"
                     /translation="MTRVLIVEDEESFADPLAFLLRKEGFTTAVAGTGQDALEEFDRN
                     GADIVLLDLMLPGMSGTDVCKALRTRSAVPVIMVTARDSEIDKVVGLELGADDYVTKP
                     YSARELIARVRAVLRRGGEGGTEVDGLPGVLEAGPVRMDVERHVVSVNGADVALPLKE
                     FDLLEYLLRNVGRVLTRGQLIDRVWGADYVGDTKTLDVHVKRLRSKVEADPSAPRHLV
                     TVRGLGYKFES"
     gene            complement(2939..4144)
                     /locus_tag="VFE39_18255"
     CDS             complement(2939..4144)
                     /locus_tag="VFE39_18255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET6260585.1"
                     /translation="MDALLCVAIAVGALALGLVLGVRWSRPRDDAIPTRVEPAPGPPM
                     ADLLQRVFRSADEGLVVLNRGGEVVLHNVRAVELGVVRLGRPDARAAAACQQVLESGS
                     SLAVDLSPLDHRGRQPAAVLAHVRPLGDGFSMVEAGDTSDAVRLEATRRDFVANVSHE
                     LKTPVGAVGLLAEAVLDAADDPVEVRRFGTKILNEATRLGNLVTELIALSRLTGAERL
                     PEMGVVDVDEVVREALARSRLSAESARIEIIVDPPTGLEVEGDLTLLVTALSNLVENA
                     IAYSPPDASVSVSRRRAGDWVEIAVTDRGIGIAPEHQKRVFERFFRVDPARSRATGGT
                     GLGLAIVKHVLANHGGEVRLWSSPGTGSTFTMRLPVHAEPDDETGTDPADAAVGNFAR
                     RPELPDRVG"
     gene            complement(4285..5028)
                     /locus_tag="VFE39_18260"
     CDS             complement(4285..5028)
                     /locus_tag="VFE39_18260"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007030857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglyceromutase"
                     /protein_id="HET6260586.1"
                     /translation="MAGMGTLVLLRHGQSVWNAENRFTGWVDVPLSETGEQEARRGGE
                     LMREHALLPDVVHTSVLRRAISTANLALDTCDRHWIPVHRDWRLNERHYGALQGKNKK
                     QTLEQFGEEQFMEWRRSYDVPPPPIEPGSEYSQDGDPRYRGRQVPRTECLADVVDRFL
                     PYWESAVVPDLHAGKVVLLAAHGNSLRALVKHLDGISDTDIVGLNIPTGIPLRYDLDD
                     DLRPVVAGGTYLDPEAAVAAAAAVAAQGR"
     gene            complement(5030..5653)
                     /locus_tag="VFE39_18265"
     CDS             complement(5030..5653)
                     /locus_tag="VFE39_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260587.1"
                     /translation="MRIGTYVIALVLAAGTALAGCSVTVQGTAAPEPAAASAAPASGP
                     APSGDVFADTQGRFGLAPPAGWAVDTSGTKGTAVVFMEPQPAGSAAERFGANINVLVV
                     QTPADLSATIAGARQELQGLSGYTSTADEAVTLHDGTPAHILGGTFVDSASRLSLRNV
                     QLFTVHDGTAIVATGTSPADAWGSYGTVFDTSLRSLTVAHVTRGPGS"
     gene            complement(5728..6246)
                     /locus_tag="VFE39_18270"
     CDS             complement(5728..6246)
                     /locus_tag="VFE39_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009079374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET6260588.1"
                     /translation="MDDDEDIWLTADELKAWLSYVAMSTLLEAALDRQLQRDSAMSHP
                     YYQILAMLSDVPGRTLRMSDLAAITQSSQSRLSHAVARLEANGWIRRIPCADDRRSTL
                     AQLTEEGFAALAAAAPGHVRTVREHLFDRLTSEQVGQLREICRSVLSGLSAERGRMKM
                     LIELIESEPSAP"
     gene            6328..6834
                     /locus_tag="VFE39_18275"
     CDS             6328..6834
                     /locus_tag="VFE39_18275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET6260589.1"
                     /translation="MPVQRLNHAVLYVRDLERSVDFYTEALGFRVVNHMPGRAAFLQA
                     EASTNDHDLGLFAVGSSAGASQAGRATVGLYHLAWEVDTLAELQRIAAVLTERDSLVG
                     ASDHGSTKALYAHDPDGLEFEVSWLVPADLLTEDDQIKTVPLDLGAEIERYGAQTKGG
                     VGVSFAAG"
     gene            complement(6837..7595)
                     /locus_tag="VFE39_18280"
     CDS             complement(6837..7595)
                     /locus_tag="VFE39_18280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET6260590.1"
                     /translation="MTARTLDRLAGEPLWAQLLADLRRRLEHDEFTVAFPGELALVSE
                     YGVSRHTVREAVRRLRDEGLVIAGRGRTPRRAEPAEIEQPLGEIFSLFSAVEATGQVQ
                     RSVVRTLDVRADGVVAARLGLEESTPLVHLERLRFADSDPLAVDRVWLPERLAAPLLD
                     VDFSRTALYAEYAARCGVRVTGGSERVRAVVPSEAERELLGVGTGVAAFVIDRLGCAG
                     ERPVEWRQTLVRADRFSVTARFSAREGYRMDGSA"
     gene            complement(7658..7789)
                     /locus_tag="VFE39_18285"
     CDS             complement(7658..7789)
                     /locus_tag="VFE39_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET6260591.1"
                     /translation="MTQRGPVRSGPLDEQLCAEIVELRQALHGLTGRLRSAAGAGAG"
     gene            complement(7849..8511)
                     /locus_tag="VFE39_18290"
     CDS             complement(7849..8511)
                     /locus_tag="VFE39_18290"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HET6260592.1"
                     /translation="MLAVEKSLRALLTQGAQDGTLGPGSKLPTERDLVERLSAPRSAI
                     RRALDVLERDGVVIRHVGRGTFLTEAGLPPAEGAPPDTSPAEIMQVRLLLEPPVAELA
                     ARVATQADLDRIAECLRGGEGSEGFEGFEAWDARLHRAIALAVHNGLLINMFDVMNTA
                     RSLPVWGSLKRRTSSPERRRRYHEEHAAIVDALRDRDPTGARDSMRRHLQNVSDNLLG
                     RH"
     gene            8654..9532
                     /locus_tag="VFE39_18295"
     CDS             8654..9532
                     /locus_tag="VFE39_18295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HET6260593.1"
                     /translation="MKLVTFSTGSDARVGVVSADGEAVHDVGELLPPSCRLLDVIGAW
                     DDLGPVLRRRAAELPALPLESVELLAPIPEPRRNVWCIGKNYREHAVEFGRSGYDTPS
                     RSEALPDKPIMFSKATTAVTGPGALVDPGPTTELDYEGELTVIIGTGGRGISREDAYS
                     HVWGYTIINDVTARDVQRDHKQWLLGKSFDTHCPMGPYAVTADEIGDVTALELETTVN
                     GERRQFAPLKDLIFDVPELIATISAGTTLLPGDLIATGTPAGVGLGFDPPRFLTSGDV
                     VEITITGLGTLSNRIR"
     gene            9545..10306
                     /locus_tag="VFE39_18300"
     CDS             9545..10306
                     /locus_tag="VFE39_18300"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET6260594.1"
                     /translation="MQVNGKELAVEVEGDGPAVLLVHGLGGTSNFYQVQTDALVERFR
                     VIRVDSAGAGRSPVADGISVESHADDLAAVLAELGVDSAKVVGHSMGTLVVRALAARH
                     PGTVSALALLGAVREPADAGRQAQRDRAAVLREKGTAAVAPGVVANALSETTRRDRPE
                     VAAFVRELVMRQDAEGYARNCEALAAATDPGPIDPGLPLLLVTGSDDKVGPPEASQEL
                     ADAHGSATVEILPGVGHWTALEAAGATTDHLLKFL"
     gene            10335..>10672
                     /locus_tag="VFE39_18305"
     CDS             10335..>10672
                     /locus_tag="VFE39_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013672557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HET6260595.1"
                     /translation="MPVPTSKTLFRGVNILDSTGADPYPGDVLVENDRIAAVGDVDPA
                     AAEGARIIEGRGRTLMSGLCDAHTHFSWNNSADLDGLGTLPVEEHLLFCIESARTYID
                     SGYTMCLGAAS"
BASE COUNT         1609 a         3851 c         3717 g         1495 t
ORIGIN      
        1 gtcacgcgac tgcgcgtcac ggggctgggg ttcacggagc agcggctccc gcacctgggg
       61 ctcccgcgac tgcgactccc acgccgacag cggctcccgc gacaagtgct cccgcgacaa
      121 gggctcccgc gacaggggtt ccggcgcccg gggctccggc gcccgcaggc gctcgcgagg
      181 ctgcgcctcc cacggctgcg gctcccgcgc ccgcggctcc cacgccggcg gctcctgcac
      241 tcgcgactgc ggctcccacg ccggttgctc ctgcacccgc gactgcggct cccactgcgg
      301 cggctgctgc acccgcgaat cgtgcggcag cgactcccac ggctgcggct cgcgctgccg
      361 aggctcccac ggctccggct cgcgcggcag ggactcccgc gcctgacgct cggaaggctc
      421 gcggacggac tcccatggct ccggctgcct acgcgacggc ggttggcccg gcactggcgg
      481 aacccgctcg cgcagcatcg aacgatcggg ctccggagcc tgcgcgggcc gccactcggg
      541 agcgaatgat ccgcttccgt tcacgcgcgg gggccccgcg tcgtcctcgt cgggtgcgcg
      601 ccgacggcgg cgacgaccgg tcgcaccggc gtcaccgtgc tcagcgagca gctcggcgac
      661 agtgcgctga cgcgggtggt cggtatcgga gctcatccgg tacaccgtgc ctcgtacgag
      721 gtcaagcgtc caagtacact cggcgcgtcc tgcgccgagc ggtccgtctg aaccgtccca
      781 tcgagcgtgt cgagcctccg gaggatgaca ccctcccgca acgcccacgg acagatctcg
      841 agctggctca cgcgcagtgc acgcatcgaa gcctcggcga ccaacgctcc ggccaccagc
      901 tgatgggacc ggctggggct gacgccttcg agctcggcca ggtcgccgga ggacatccgg
      961 gtgatgaacg cggccagctg ccgcagcccg acgtcggtga gtgaccggtg cacccgtggg
     1021 cccgcgctcg acggcgccgc accggtgagc cgggccagcg agcggaacgt cttggacgtg
     1081 ccgacggcga gctcgggctc cccggcgcgg cgcagcctgt gggccacggg ggcgagctgg
     1141 gcgtcgaggt gatcacgcag agcggcgatc tcgcggcggg acggtgggtc ggagcggaac
     1201 cactcgcggg tgagccgccc cgctcccatc gggagcgact ccgcgacagc ggggtgctcg
     1261 tcgagaccgg ccgcgatctc cagcgagcca cctccgatgt cgagcaccag caaccggcct
     1321 gccgaccagc cgtaccagcg gcggacggcg aggaaggtgt accgggcctc gtcctcaccc
     1381 ggaagaacct gcagtgcgac accggtctgg tcgcgcacct ttgccagcac cgccgcggag
     1441 ttcgcggcgt cacgcagcgc ggaggtggcg aacgcgagca gatcggcgca gccggccgcg
     1501 gcggcggcca cgctcgcccg ctgcacggcc cgcaccagcg cgtccgcacc ggctttcgtg
     1561 agcgcgcccg acacgtcgag gttctcggcg agccgcagct cggtcttgtc ggacgtcatc
     1621 ggcgtgggat ggccaccacg gtgcgcgtcc accacgagaa ggtggacggt gttggaaccg
     1681 acgtcgagca caccgaggcg cacgtgatca ggctactgcc ccgcgtgatg tccccgtgac
     1741 cgaacccgtc acaaccagca acactcgatc accgccagcc acatttggca cggatccccg
     1801 cccccaaacc cactgatcaa ggcacaaacg tcgcaagtcg accgtgattc acagctcttg
     1861 tgccttgatc accgggatgt gcgcggcagg gcaggttgtc cacatgtgca cgcagttgtc
     1921 cacaggtttg ccatcgccgc ccgccagccg tcggtaccga gtgatcgcct gtggatgcga
     1981 cagcctctcc gcatcagtcc cggagtgatc cgggaccacc tcgaagatca tttcctgagg
     2041 agttctgaag caccaggcca ggcacatcgc tcgcttcacg ccggatgtct catgagacat
     2101 gctgacgcga cgaacaggtc ttcgccgacc tcatggccac ggtcggcgcg gccgtcacac
     2161 gccgaagttc gaggcggggc gggctagggg tgcggggcgg gggctggccg ggcgggggct
     2221 ggtgagcggg gcggtcagct ctcgaacttg tagcccaggc cccgcacggt caccaggtgc
     2281 cgaggagcgg aggggtcggc ctcgaccttc gagcgcagcc gcttgacgtg gacgtcgagt
     2341 gtcttggtgt cgccgacgta gtcagcgccc cagacgcggt cgatcagctg tccgcgggtg
     2401 agcaccctgc cgacgttgcg cagcaggtac tcgagcaggt cgaactcctt gagcggcagg
     2461 gccacgtcgg ctccgttcac cgacacgacg tgccgctcga cgtccatccg caccgggccg
     2521 gcctccagca cgcccggcag gccgtcgacc tccgtcccgc cttcaccgcc gcggcgcagg
     2581 acggcgcgga cgcgggcgat gagctcacgg gcggagtagg gcttggtgac gtagtcgtcg
     2641 gcgcccagct cgagcccgac caccttgtcg atctcgctgt cgcgggccgt caccatgatc
     2701 acggggacgg cggagcgggt gcggagcgcc ttgcagacgt cggtgccgct catgccggga
     2761 agcatgaggt cgagcagcac gatgtcggcg ccgttgcggt cgaactcctc gagcgcgtcc
     2821 tgccccgtgc cggcgacggc cgtggtgaac ccctccttgc ggagcaggaa cgcgagcggg
     2881 tcggcgaagg actcctcgtc ctccacgatg agcactctgg tcatggggtc ctcccagctc
     2941 acccgacgcg gtcgggtagt tcgggccgcc gggcgaagtt gcccacggcg gcgtcggcag
     3001 ggtccgtgcc ggtctcatcg tcgggttcgg cgtggacggg tagccgcatg gtgaaggtag
     3061 atccggtccc cgggctgctc cagagccgga cctcgccacc atggttggcg agtacgtgct
     3121 tgacgatcgc gaggccgaga cccgtgccgc cggtggcccg ggaccgggcg gggtcgacgc
     3181 ggaagaaccg ctcgaagacc cgtttctgat gctcgggcgc gataccgata ccgcggtcgg
     3241 tgaccgcgat ctccacccag tcgcccgctc ggcgccgcga cactgacacg gaggcatccg
     3301 ggggtgagta ggcgatcgcg ttctcgacga ggttcgacag tgcggtgacg agcagcgtca
     3361 ggtcgccctc gacctccagc cccgtcggcg ggtccacgat gatctcgatc cgggcggact
     3421 cggcggagag cctggagcgg gccagcgcct cgcgcacgac ctcgtcgacg tcgacgactc
     3481 ccatctcggg cagtcgctcg gcaccggtca gccgggagag cgcgatcagc tcggtgacga
     3541 ggttgcccag ccgggtggcc tcgttgagga tcttggtgcc gaatcggcgc acctcgaccg
     3601 ggtcgtcggc ggcgtcgagc accgcctcag ccagcaggcc gacggccccc acgggcgtct
     3661 tgagctcgtg gctgacgttg gccacgaagt cgcgacgggt ggcctcaagc cggacggcgt
     3721 cggaggtgtc gcccgcctcg accatgctga agccgtcacc gagcgggcgg acgtgggcaa
     3781 gtacggcggc gggctggcgg ccgcggtggt cgagcgggga caggtcgacc gccaacgacg
     3841 acccggactc gagcacctgc tggcaggcgg cagcagcgcg ggcatcgggg cggcccaggc
     3901 ggacgacgcc gagttcgacg gcgcgcacgt tgtgcagcac cacctcacca ccccggttca
     3961 gcaccaccag accctcgtcc gccgaccgga agacccgttg gagaaggtcg gccatgggag
     4021 ggccgggcgc gggttccaca cgggtcggga tggcgtcatc gcgggggcgg ctccaccgga
     4081 ccccgagtac gaggccgagc gcgagcgctc cgaccgcgat cgccacgcac aggagcgcgt
     4141 ccacggcggc atcgtaggca ggccagccac atatcgggcc agttcaggag ccgttcgtga
     4201 cgctgatgac gccggagacg gcagatttcg accctcgttc acgatccgtt catccgttcg
     4261 gggacgaggc ggacaggccg ccggtcaacg cccctgggct gccaccgcgg cggcagccgc
     4321 gacggcggcc tccgggtcga ggtacgtgcc accggccacc acggggcgca ggtcgtcgtc
     4381 gaggtcgtag cgcagcggga tacccgtggg gatgttcagc ccgacgatgt cggtgtccga
     4441 gatcccgtcg aggtgcttga cgagcgcccg cagcgagttg ccgtgggcgg cgagcagcac
     4501 gaccttgccg gcatggaggt cgggcacgac cgccgactcc cagtagggca ggaaccggtc
     4561 gaccacatcg gcgaggcact ccgtgcgcgg aacctgcctg ccccggtagc gcgggtcgcc
     4621 gtcctggctg tactccgacc cgggctcgat ggggggtggt gggacgtcgt aggagcggcg
     4681 ccactccatg aactgctcct cgccgaactg ctcgagcgtc tgcttcttgt tcttgccctg
     4741 cagagcgccg tagtgccgct cgttgagccg ccagtcacgg tgcacgggga tccagtgccg
     4801 gtcgcacgtg tcgagcgcca ggttcgcggt ggagatcgcg cggcgcagca ccgaggtgtg
     4861 cacgacatcg ggcagcaacg cgtgctcgcg catcagctca ccgccgcggc gggcctcctg
     4921 ctcacccgtc tccgagagcg gcacgtcgac ccagccggtg aagcggttct cggcgttcca
     4981 cacgctctgc ccgtgtcgca gcagcaccag tgttcccata ccggccactc taagagccgg
     5041 gaccacgggt cacgtgggcc accgtcaagc tgcgcaacga cgtgtcgaac accgtcccgt
     5101 aggaccccca tgcgtccgcc ggcgacgttc ccgtggccac gattgccgtg ccatcgtgca
     5161 ccgtgaacaa ctgcacgttg cgcagcgaga gccgggacgc ggagtcgacg aacgtgcccc
     5221 cgaggatgtg tgcgggcgtg ccgtcgtgca gggtgacggc ctcgtccgcg gtcgaggtgt
     5281 agccgctgag gccctgcagt tcctggcggg caccggcgat cgtggccgac aggtccgcgg
     5341 gcgtctgcac gaccagcacg ttgatgttgg cgccgaaccg ctcggcagcc gatccggccg
     5401 gctgaggttc catgaacacc accgcggtgc ccttggtccc gctggtgtcg acggcccatc
     5461 cggctggcgg ggcgagcccg aagcggccct gggtgtcggc gaacacatca ccggacgggg
     5521 ccggcccgga cgccggggcg gcggacgccg ccgcaggctc gggagccgcg gtgccctgga
     5581 cggtgaccga gcagccggcc agcgccgtgc ccgccgccaa caccagcgcg attacgtacg
     5641 tgccgatccg cattccgccc cctccacgat gggtagccat gatcacccat cacggcgccg
     5701 gagaaaccag gacccgcgga ggctgcatca gggggccgat ggctccgact cgatgagctc
     5761 gatcagcatc ttcatccgcc cccgctcggc cgacagcccc gagaggacgc tgcggcagat
     5821 ctcgcggagc tgcccgacct gctcagaggt cagccggtcg aacaagtgct cacgcacggt
     5881 gcggacatgg ccgggcgcag cggcggccag cgccgcgaag ccttcctcgg tgagctgggc
     5941 gagcgtactg cgccgatcat cggcgcaggg tatccgacgg atccagccgt tggcttcgag
     6001 ccgggccacc gcgtgggaga gccggctctg cgagctctgc gtgatggcgg cgaggtcact
     6061 catccgcagc gtccgccccg ggacgtccga cagcatggcc aggatctggt aatacggatg
     6121 ggacattgcg gaatcgcgct gcagctgccg gtccagagcc gcttccagca gcgtggacat
     6181 ggcgacatag gacagccacg ccttcaactc gtcggccgtc agccagatgt cctcgtcatc
     6241 gtccacccga ttagggtacc tagaaagttg agccctcacg ttttctgccg taccgtctct
     6301 ttcgacactg accggtcgga ggactggatg cctgtccaac gtctcaacca tgccgtgctc
     6361 tacgtacgcg accttgagcg gagcgtcgac ttctacaccg aggcactggg tttccgcgtc
     6421 gtcaaccaca tgccggggcg ggccgcgttc ctgcaggccg aggcctcgac gaacgaccac
     6481 gacctcggcc tgttcgccgt cggctcgagc gcgggggcct cgcaggccgg ccgcgccacg
     6541 gtggggctct accacctggc ctgggaggtc gacacactcg ccgagctgca gcgcatcgcg
     6601 gccgtactca ccgagcgcga ctcgctggtg ggcgcctcgg accacggatc cacaaaggcg
     6661 ctctacgccc acgatcccga cggcctcgag ttcgaggtga gctggctcgt gcctgccgac
     6721 ctgctcaccg aggacgacca gatcaagacg gtgccgctcg atctcggggc agagatcgag
     6781 cgatacggcg cgcagaccaa ggggggcgtc ggcgtctcct tcgccgccgg ctgacgtcag
     6841 gcgctgccgt ccatgcggta gccctcccgc gccgaaaagc gtgccgtgac actgaaccgg
     6901 tcggcccgga cgagcgtctg cctccactcg accgggcgct ctcccgcaca gccgaggcgg
     6961 tcgatcacga acgccgccac gccggttccg acgccgagca gctcgcgctc cgcctcgctg
     7021 ggcaccaccg cccgcacccg ctcactcccg ccggtcaccc gaacaccaca gcgggcggcg
     7081 tactcggcgt agagggccgt gcgactgaag tcgacgtcga ggagcggcgc agcaagccgc
     7141 tccggcagcc agacgcgatc gacggcgagc gggtcggagt cggcgaagcg caggcgctcg
     7201 aggtgcacga gcggcgtcga ctcctccagc ccgagccgag ctgcgaccac cccgtcggcg
     7261 cgcacgtcga gcgtccggac gacgctgcgc tgcacctgac cggtggcctc caccgccgag
     7321 aacaggctga agatctcccc gaggggctgc tcgatctccg cgggctccgc ccgccgcggg
     7381 gtacggccgc ggcctgcgat gacaaggccc tcgtcacgca gccgccgcac cgcctcgcgg
     7441 acggtgtgcc ggctgacgcc gtactccgag accagcgcca gctcgccagg aaaggccacg
     7501 gtgaactcgt cgtgttccag gcggcggcgc aggtcggcca gcagctgcgc ccataggggt
     7561 tcaccggcga gacggtccag cgtccgggcc gtcatgtggc gccctccgct gctggcatcg
     7621 taatgaggtt ggagctcatg gggtccggtc acggcaatca gcccgcgccg gcgcctgcgg
     7681 ccgagcggag ccgaccggtg agcccgtgca gtgcctggcg gagctcgacg atctcggcgc
     7741 acagctgctc gtcgagggga ccggaccgga ccggtccgcg ctgggtcatg ctgccaccgt
     7801 atgctgccac gccgatctcg ggcaccccgg atccgcccag ctgggcactc aatgcctgcc
     7861 cagcaggttg tcggagacgt tctggagatg ccggcgcatg ctgtcacggg caccggtcgg
     7921 atcgcgatcg cgcagagcgt cgacgatcgc tgcgtgctct tcgtggtagc gccggcgccg
     7981 ctcggggctg gacgtgcggc gcttgaggct gccccacacc ggcaggctgc gagcggtgtt
     8041 catgacgtcg aacatgttga tgagcaggcc gttgtgcacg gccaaggcga tggcgcgatg
     8101 cagtcgggcg tcccaggcct cgaagccctc gaagccctcg cttccctcgc cgccgcgcag
     8161 gcactcggcg atgcggtcga gatcggcctg ggtggccacc cgcgcggcga gctcggccac
     8221 cggcggctcg agaagcagcc ggacctgcat gatctccgcc gggctggtgt cagggggcgc
     8281 gccctccgcc ggcggcagcc ctgcctcggt gaggaacgtg ccgcgcccga cgtgccggat
     8341 cactacgccg tcgcgctcga gaacgtcgag ggcacgccgg atcgcgctgc gcggagcgga
     8401 cagccgctcc acgaggtcgc gctcggtggg gagcttggag cccgggccga gcgtgccgtc
     8461 ctgcgcgccc tgcgtgagca gggcacgcag cgacttctcc acggccagca tgcggatacc
     8521 gtaccgcatg ttgaaccaat gacgaccaat ttgctcacgt cgatcgacat cggtcgtccc
     8581 ctcgacagca gtgacaggaa gccgtacggt cgaccaggtc cagattggtt taattggttg
     8641 agattggtga gcaatgaagc tggtgacgtt ctccaccgga tccgacgcgc gggtcggtgt
     8701 ggtcagtgcc gacggggagg ctgtccacga cgtcggcgag ttgctgccac cgagctgccg
     8761 gctgctcgac gtgatcgggg cgtgggacga cctcggcccg gtgctgcgcc ggcgggcggc
     8821 cgagctgccg gcactgccgc tggagtccgt cgaactgctc gcaccgatcc ccgagccgcg
     8881 gcgcaacgtc tggtgcatcg gcaagaacta ccgtgagcac gccgtggagt tcgggcgcag
     8941 cggctacgac accccgagcc gatcggaggc cctcccggac aagccgatca tgttctcgaa
     9001 ggccaccacg gcggtgaccg gtccgggtgc tcttgtggac cccggtccca ccaccgagct
     9061 ggactacgaa ggcgagctca ccgtcatcat cggcacgggc ggccggggca tctcccgcga
     9121 ggacgcctac agccacgtct ggggctacac gatcatcaac gacgtcacgg cgcgggacgt
     9181 gcagcgcgac cacaagcagt ggctgctggg caagtcgttc gacacccact gcccgatggg
     9241 gccctacgcc gtcacggccg acgagatcgg cgacgtcacc gcgctcgagc tggagaccac
     9301 cgtcaacggg gagcgccggc agttcgcgcc gctcaaggac ctgatcttcg acgtgcccga
     9361 gctcatcgcc acgatctccg ccggtaccac gctgctgccc ggcgacctca tcgccacggg
     9421 cacgcccgcc ggggtcggcc tcggtttcga cccgccgcgc ttcctcacca gcggcgacgt
     9481 cgtcgagatc acgatcaccg gcctgggcac gctgtccaac cgcatccgct gagagaggac
     9541 ccctgtgcag gtcaacggta aggaactcgc cgtcgaggtc gagggggacg gccccgccgt
     9601 gctgctcgtg cacggcctcg gtggaacctc gaacttctac caggtccaga cggacgcgct
     9661 cgtcgagcgc ttccgcgtga tccgcgtcga ctccgcgggc gcgggccgct cgccggtggc
     9721 tgacgggatc agcgtggaaa gccacgccga cgacctcgcc gccgtgctcg ccgagctcgg
     9781 tgtcgacagc gccaaggtgg tcggacactc gatgggcacc ctcgtcgtgc gcgcgctcgc
     9841 cgcccgccac cccggcaccg tgtccgcgct cgcgctgctc ggggcggtgc gggagcccgc
     9901 cgacgccggc cggcaggccc agcgcgaccg cgccgcggtc ctgcgggaga agggcactgc
     9961 cgccgtcgcc cccggcgtcg tcgccaacgc cctctccgag accacccgcc gcgaccgccc
    10021 ggaggtcgcg gcgttcgtcc gcgagctcgt gatgcgccag gacgcagagg gctatgcccg
    10081 caactgcgag gccctcgccg cagccaccga tcccggcccg atcgaccccg gcctgccgct
    10141 gttgctcgtg accggctccg acgacaaggt cggaccgccc gaggcgagcc aggagctcgc
    10201 cgacgcgcac ggctcagcga ccgtggagat cctgccgggc gtcgggcact ggaccgcgct
    10261 cgaggccgcc ggcgccacca ccgaccacct gctcaagttc ctgtagttcc accctcagcg
    10321 acgaggagtc caccgtgccc gtcccgacga gcaagaccct gttccgcggc gtcaacatcc
    10381 tcgactccac cggagccgat ccctaccccg gcgacgtgct ggtcgagaac gaccgcatcg
    10441 ccgccgtcgg cgacgtcgac cccgccgccg ccgagggcgc ccgcatcatc gagggccgag
    10501 ggcgcaccct gatgtccggg ctgtgcgacg cccacaccca cttcagctgg aacaacagcg
    10561 ccgacctcga cggcctgggc accctgcccg tcgaggaaca cctcctgttc tgcatcgaat
    10621 cggcccgcac ctacatcgac tccggctaca caatgtgcct cggggccgcc tc
//
LOCUS       DASRSC010000377        12150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Pseudonocardia sp. isolate SMAG_U5261 k141_3765269,
            whole genome shotgun sequence.
ACCESSION   DASRSC010000377 DASRSC010000000
VERSION     DASRSC010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37970118
            Sequence Read Archive: SRR11834729
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Pseudonocardia sp. (soil metagenome)
  ORGANISM  Pseudonocardia sp.
            Bacteria; Actinomycetota; Actinomycetes; Pseudonocardiales;
            Pseudonocardiaceae; Pseudonocardia.
REFERENCE   1  (bases 1 to 12150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 23:25:49
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,672
            CDSs (total)                      :: 3,655
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 17
            tRNAs                             :: 17
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 58
            CDSs (without protein)            :: 58
            Pseudo Genes (ambiguous residues) :: 0 of 58
            Pseudo Genes (frameshifted)       :: 19 of 58
            Pseudo Genes (incomplete)         :: 49 of 58
            Pseudo Genes (internal stop)      :: 6 of 58
            Pseudo Genes (multiple problems)  :: 15 of 58
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12150
                     /organism="Pseudonocardia sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3765269"
                     /isolate="SMAG_U5261"
                     /isolation_source="soil"
                     /db_xref="taxon:60912"
                     /environmental_sample
                     /country="USA: California"
                     /lat_lon="35.5 N 121 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            233..3613
                     /locus_tag="VFE39_18310"
     CDS             233..3613
                     /locus_tag="VFE39_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020420078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HET6260596.1"
                     /translation="MGWNNPDVPWSKLEAALSGRPRDGASLTARSAGGERIDTGGPEV
                     DGGDSPAWSRKREPYTPPELPEPESRVPYAELHCHSNFSFLDGASHPEELIEQAARLG
                     LDAIALTDHDGMYGVVRFAEAAAELGVRTVFGAELSLGLTAPQNGVADPEGSHLLLLA
                     RHPEGYRSLCRTISTAQLRGKEKGRPVYDLDEVVADTAGQVLVLTGCRKGAVRQALRT
                     GGRDAAGKELRRLVERFGADHVAVELTHQALPTDSERNDALADLARDAGLPTIASTAA
                     HYAAPHRFPLATALAAVRSRRSLDDADGWLPPAGTAHLRSGAEMAARFDQRYPGAVAR
                     AAAVGVECAFSIDLVAPELPPFDVPPGHTEATWLRELTFRGVARRYGSYAEHPEAVET
                     VERELAIIEKKNFPGYFLIVHDIVEFCRRSDILCQGRGSAANSAVCYALGITNVDAVA
                     HRLLFERFLSPARDGYPDIDLDIESGRREEVIQYVYHRHGRGCAAQVANVITYRPRSA
                     VRDMAKALGFSPGQQDAWSKQIERWHGPVGDTAIAPAGMPGQVVELADELLGFPRHLG
                     IHSGGMVICDRPVSEVVPVEWARMEGRTVVQWDKDDCASAGLVKFDLLGLGMLTALHL
                     MIDLVAERHGEKIELHELQPTDPDVFAMLQRADSVGVFQVESRAQMATLPRLKPRIFY
                     DLVVEVALIRPGPIQGGSVHPYIQRRNGAEWKHDHPLLANALDKTLGVPLFQEQLMQV
                     AVDVAGFTAADADELRRAMGSKRSEEKMERLRARFFDGMAANGITGEVADGIFTKMLA
                     FANFGFPESHSISFASLVYYSAWFKHYHPAAFCAALLNSQPMGFYSPQSLVADARRHG
                     VLTRGPDVNLGRAEAVLQPDQRSRGGQTIRLGLAEVRGIGLELAEEIDAERERGGPYR
                     DLADLARRVRLTVPQAEALATAGAFGCFGVDRRSALWAAGVVAAVRPEQLPGTAVGLE
                     APALPGLTGVELTAADVWATGVSPDSHPMQHLRERLDGLGAVRIDGLDAVGRDLVAGD
                     PEQTPPRVLVGGLVTHRQRPATARGVTFLNLEDESGMLNVTCSEGLWARYRAVAVGST
                     ALLVRGRLERSPEGVLNLVADRLQRLVLTVPVKSRDFR"
     gene            complement(3651..4649)
                     /locus_tag="VFE39_18315"
     CDS             complement(3651..4649)
                     /locus_tag="VFE39_18315"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET6260597.1"
                     /translation="MRTTVRKPRGRHNAKDQTTASVRNRASWRRYGAVMAFDLVDLQL
                     FVQVVDSGSITDGARRCHLSLPSASARIRLMERELGTSLLQRHRRGVHPSAAGQQLLG
                     HARTVIGQLSRMRGDLAAFADGMHADLTLLANTAAASTFLPDALIRFLAEHPDTDVNL
                     EERPSQQIVGAIAEGRAELGVVADTVELGLLHTLPLRPDPVVAVTTPGHPLTARHRIA
                     FAECLTFPFVGLVEGNPFQEHLGGRAQPLGLRPRYRARLSDPDAICRAVAAGIGVAVL
                     PAAVVSRLRRELAITTIPLTDSWADRRLLLCTTDPGRLSSPARALATHLTHELRVP"
     gene            4648..5988
                     /locus_tag="VFE39_18320"
     CDS             4648..5988
                     /locus_tag="VFE39_18320"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET6260598.1"
                     /translation="MASARDGWSLIGICLGFFVVLLDATIVNVALGAIEAALGGGLGG
                     QQWVVGAYTVAFGALLLSAGAAGDRCGSRRMYLLGLALLAGAGMACALAGDLPQLLIA
                     RVVQGAGAALVTPCSLALITHRFPDARARARALGAWGGISGMGLAAGPVLGGLLVDAA
                     GWRAVFWAVVPFAVLAAALVVATTPETPRRTAVRPDLVGQALAAVALTALTAALVTGP
                     SAGWNSSMALMAAGAGLASAVAFAASQRLVRAPMIPPSLFADSQFGVAVGVGALFNFG
                     LYGTLFCVALYLEQTLVTSTRTTGLVLLPLTVVVAICAALSGRINARTGPRVPMVLGL
                     GAGVLGSVALAGMGAGSAPVVVGAAAALLGGVGLAMPAMTSVAVSAVPAASAGLGAGV
                     LNAARQAGGAVGVAGLGAVLLDPAGRPSLLAAMLVVAAGYVLALLLTVGLIRTP"
     gene            complement(6057..6710)
                     /locus_tag="VFE39_18325"
     CDS             complement(6057..6710)
                     /locus_tag="VFE39_18325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR1/FUN34/YaaH family transporter"
                     /protein_id="HET6260599.1"
                     /translation="MSVSDARPRTGNVTREASATAGGNPALLGLPALLPGSLTLGLWL
                     IGYLDTPTLPGVMIPAITSSSGLFLLVACVWAARVGQGAAAAIFGTFAAFWLSFGFLL
                     LGVINGWFGIATEPSLRAEQIREVQSTFVLSWLIVFATLTVATLRLPLAFTAAFAVVC
                     TTLALVLGFVVNGNPLFATLGGITTFVFCAIYAYIFVDAMTQELGGEAMPMGAPIQQ"
     gene            complement(6988..7311)
                     /locus_tag="VFE39_18330"
     CDS             complement(6988..7311)
                     /locus_tag="VFE39_18330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007029509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HET6260600.1"
                     /translation="MILIVLRAKIRPEKHDEWLAGIRQYTADVRSEPGNLSFDYFQNG
                     DDPNEFAMIEVFADSEAGAAHVATDHATKFFAWMPAMVAERPRINYQELDGEAWTEMA
                     EVTPE"
     gene            7500..8129
                     /locus_tag="VFE39_18335"
     CDS             7500..8129
                     /locus_tag="VFE39_18335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HET6260601.1"
                     /translation="MHDDTGYDGAGYDDSDYIDLDLSEVQVGDEPWRQRRFVGCRFVD
                     ADLRGLVTESCTFDECDFTGADLGDSSHRGSAFRTCMIRRATLGGATFRGCSLLGTSL
                     IDCRLRPLVVTDCDLTLAALGRADLRKTDLSGLRLREANLVEADLREADLRGADLSGA
                     RLLHTRLEGADLRGARINPDGLVQARLTGATVDAEQALAFAVAHGLRIA"
     gene            8156..9061
                     /locus_tag="VFE39_18340"
     CDS             8156..9061
                     /locus_tag="VFE39_18340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET6260602.1"
                     /translation="MGHPAITQGRRAGWENGAVATLMDGKATLNEILGELASRVEKLQ
                     ANGVTPGLGTLLVGDDPGSHSYVRGKHRDCAKVGITSIQKELPADATQADVERAIDEL
                     NADPACTGFIVQLPLPQGLDAGAALERIDPAKDADGLAPVSLGRLVLGEPGPLPCTPR
                     GIVELCRRYGVELAGARVTVVGRGVTVGRPLGLLLTRRSENATVTLCHTGTRDLAAEV
                     RRADVVVAAAGVPGLITADMVRPGATVLDVGVTRTDLGLVGDVAPEVVEVAGRLAPMP
                     GGVGPMTRAMLLTNVVEAAERSLSE"
     gene            9156..9419
                     /locus_tag="VFE39_18345"
     CDS             9156..9419
                     /locus_tag="VFE39_18345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009949964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3017 domain-containing protein"
                     /protein_id="HET6260603.1"
                     /translation="MHAPMILVMVIAAAGMVRVLTQHWREGAVLLGGALIAAAVLRVL
                     LPVDRVGLLAIRSRATDVLCYTAFGVVMTALAVTITRGSFDFP"
     gene            9667..10980
                     /locus_tag="VFE39_18350"
     CDS             9667..10980
                     /locus_tag="VFE39_18350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018684206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HET6260604.1"
                     /translation="MTDDARAARIVDTLVEAFSDLMAADPAAFRTKFRKMAADPFAFY
                     RGSACVFYADMAERDDPWADERTSRVWIQGDLHAENFGTYMDGDGVLIFDVNDFDEAY
                     VGHFTWDVTRFAASLALLGWRKAIPDPDITTLVERYTRAYLDQVRWFVDQADDRSVSL
                     RLATTDGVVQQVLQLARLRTRVELLDKITEPEGFDRILRDGPGLRRLDDGERAMVTDA
                     FERYLDSIPESKKFRGVTYTVKDVAGRSGFGIGSAGLPAYTVLIEGYNQALDNDVVLS
                     MKQGNVAAPSRVVTDPAIAAGFRHHGHRTVVSQRALQAHADPLLGWTDLDGVGFVVSE
                     VSPYEADLDWSELTEPSEIAPVVDYLGRATAKVHCVADSDADHSVVPFQTEDAIMGVV
                     AGREDEFVAWVVEFAHEYAAQVRADHALFVEAFRSGAIPGVASTS"
     gene            complement(11024..11965)
                     /locus_tag="VFE39_18355"
     CDS             complement(11024..11965)
                     /locus_tag="VFE39_18355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET6260605.1"
                     /translation="MIIQTEALRREFLVGTRLRRRRVPAVEDVTLTVARGEAVGFLGP
                     NGAGKSTTIKMLTGVLVPTSGRASVCGLDPVRERRELARRIGVVFGQRSQLWWDLPLV
                     ESFDLHGAIHRVPAARYRERLDECVELLDMGGFLRTPVRQLSLGQRMRGEVTAALLHS
                     PELLVLDEPTIGLDLTSKERLRSFLSAMNARGDVTLLLTTHDLPDVERLCRRVVVIDQ
                     GRVLVDDELAVLRQRFPGHRALVVELMAPAPPLLGLTGVVDVTVEAQGLRQRLEFSPG
                     ATTAAELIAAVAQHVELRDVSVFESSIEDLVRTIYLR"
     gene            complement(11962..>12150)
                     /locus_tag="VFE39_18360"
     CDS             complement(11962..>12150)
                     /locus_tag="VFE39_18360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET6260606.1"
                     /translation="MCYVVPGAFVAYFPALALLGRPDPLGLPEALQWSSPLVAAIAVT
                     GAGLVWRTGVRHYQGTGS"
BASE COUNT         1704 a         4211 c         4466 g         1769 t
ORIGIN      
        1 ttggcgcccg ggtgatgatt ggggaccggg agcgcggttg tggacgccgg cggtcccggt
       61 tcggcgatca tcgccgccac gcgggctctg tgccgttcgg gacggcaccg cggcgtaggc
      121 ccggcccggg ctgtcgccgc atcaggttgg ccacgcgagg accttggccc tgtgacgagt
      181 tcgcctggtc gggcggttga gctgatcatc gaacacatgt tcgactgagc gcgtgggctg
      241 gaacaacccg gacgtaccgt ggagcaagct ggaggcggcg ctctcagggc gcccgcgcga
      301 cggcgcgagc ctcaccgcgc ggagcgcagg cggagagcgc atcgataccg gaggtccgga
      361 ggtcgacggc ggtgacagcc cggcgtggtc gcgcaagcgt gagccctaca ccccgccgga
      421 gctcccggag ccggagtccc gcgtcccgta cgccgagctg cactgccact ccaacttcag
      481 cttcctcgac ggggcgagtc accccgagga gctgatcgaa caggctgctc ggctcggcct
      541 cgacgcgatc gcgctcaccg accacgacgg catgtacggg gtggtccggt tcgccgaggc
      601 cgccgccgag ctgggggtcc gcacggtctt cggcgccgag ctgtccctcg ggctcacggc
      661 cccgcagaac ggcgtggccg atccggaggg cagccacctg ctgctgctcg cccgccaccc
      721 cgaggggtac cgctcgctgt gccgcaccat cagcaccgcc cagctgcgcg gcaaggagaa
      781 ggggcgcccg gtctatgacc tcgacgaggt cgtggccgac accgcgggac aggtgctggt
      841 gctcacgggt tgccgcaaag gtgcagtgcg ccaggcgctg cgcaccggcg gtcgtgacgc
      901 cgcgggcaag gagctgcgcc gcctggtcga gcggttcggg gccgaccacg tggccgttga
      961 gctcactcat caggccctgc ccaccgacag cgaacgcaac gacgccctcg ccgatctggc
     1021 gcgcgacgcc ggcctgccga cgatcgcgtc cacagcagcg cactacgccg ccccgcaccg
     1081 gttcccgctg gccacggcgc tggcggcggt gcggtcccgg cgcagcctcg acgacgccga
     1141 cggctggctg ccacccgcag gcaccgccca cctgcgctcg ggcgcggaga tggcggcccg
     1201 gttcgaccag cgctacccgg gtgcggtggc ccgggcggcg gcggtcgggg tggagtgtgc
     1261 gttctcgatc gacctggtag cgccggagct gccgccgttc gacgtcccac caggacacac
     1321 ggaggccacc tggctgcggg agctgacctt ccgcggggtc gcccgccgct acggcagcta
     1381 cgccgagcac ccggaggccg tcgagacggt cgagcgggag ctcgcgatca tcgagaagaa
     1441 gaacttcccc ggctacttcc tgatcgtcca cgacatcgtc gagttctgcc gccgctccga
     1501 catcctctgc cagggccggg ggtcggcggc caactccgcg gtctgttacg cgctgggcat
     1561 caccaacgtc gacgccgtgg cgcaccggct gctgttcgag cggttcctgt ctcccgcccg
     1621 ggacggctac cccgacatcg acctcgacat cgagtccggt cgccgcgagg aggtgatcca
     1681 gtacgtctac caccgccacg gccgtggttg cgccgcccag gtggccaacg tgatcaccta
     1741 tcggccgcgc tcggccgttc gcgacatggc aaaggcgctg gggttctccc cgggccagca
     1801 ggacgcgtgg agcaagcaga tcgagcgctg gcacggcccg gtgggcgaca ccgcgatcgc
     1861 gccggccggg atgcccggcc aggtcgtcga gctggcggac gagctgctcg gcttccctcg
     1921 ccacctcggc atccattccg gtggcatggt catctgcgac cggcccgtct cggaggtggt
     1981 gcccgtcgag tgggcgcgga tggagggccg caccgtcgtc caatgggaca aggacgactg
     2041 cgcgtctgcg ggcctggtca agttcgacct gctcggactg ggcatgctca ccgcgctcca
     2101 cctgatgatc gacctggtcg ccgagcgcca cggcgagaag atcgagctgc acgagctgca
     2161 gcccaccgac cccgacgtgt tcgcgatgtt gcagcgcgcc gactcggtcg gggtgttcca
     2221 ggtggagtcg cgtgcgcaga tggccacgct gccccggctg aagccacgga tcttctacga
     2281 cctggtcgtc gaggtggcgc tgatccggcc cggccccatc cagggcggtt cggtgcaccc
     2341 ctacatccag cggcgcaacg gggcggagtg gaagcacgac cacccgctcc tggcgaacgc
     2401 gctggacaag acgctcggcg tgccgctgtt ccaggagcag ctcatgcagg tggccgtcga
     2461 cgtcgcgggt ttcaccgccg ccgacgccga cgagctgcgc cgcgccatgg gctccaagcg
     2521 ctccgaggag aagatggaga ggctacgggc gcggttcttc gacgggatgg cggccaacgg
     2581 catcaccggc gaggtggccg acgggatctt cacgaagatg ctggcattcg ccaacttcgg
     2641 gttcccggag agccactcga tcagcttcgc gtcgctggtc tactacagcg cctggttcaa
     2701 gcactaccac cccgcggcgt tctgcgcggc gctgctcaac tcccagccga tgggcttcta
     2761 ctcgccccag tcactcgtgg ccgacgcccg ccggcacggc gtgctcacgc gggggccgga
     2821 tgtcaatctc ggccgggccg aggcggtgct gcaacccgat cagcggagca ggggtgggca
     2881 gacgatccgg ttggggcttg cggaggtgcg ggggatcggt ctcgagctgg ccgaggagat
     2941 cgacgccgag cgcgagcggg gcgggcccta ccgcgacctc gccgacctgg ctcgccgggt
     3001 gcgcctcacc gtgccgcagg ccgaggcgct cgccactgcg ggggcgttcg gctgcttcgg
     3061 tgtggaccgc cgttcggcgc tgtgggcggc gggggtggtg gccgcggtgc gccccgagca
     3121 gttgcccggc accgccgtgg ggctggaggc gcccgcgctg cccggtctca ccggcgtcga
     3181 gctcaccgcc gccgacgtgt gggcaacggg tgtctccccg gacagccacc ccatgcagca
     3241 cctgcgtgag cggctcgacg gtctgggcgc cgtgcgcatc gacggtctcg acgccgtcgg
     3301 acgtgacctc gtggccggtg acccggagca gaccccgccg cgggtgctgg tcggcgggct
     3361 cgtgacgcac cggcaacggc cggccaccgc gcgcggtgtc acgttcctca acctggagga
     3421 cgagagcggg atgctcaacg tcacgtgctc ggaggggctg tgggcgcgct accgcgcggt
     3481 agcggtcggc agcacggcgc tgctggtccg cggacggctc gagcgcagcc cggagggcgt
     3541 gctcaacctg gtggccgacc ggctccagcg gttggtcctc accgtgccgg tgaagtcgcg
     3601 cgacttccgc tgaggtcgtg tggtgggcag ccctgccgcc tgatcgcggt tcacggaacg
     3661 cggagctcat gggtgaggtg cgtggcgagc gcacgagcag gtgaggaaag cctcccgggg
     3721 tcggtggtgc acagcagcaa ccggcgatct gcccagctgt cggtgagcgg gatggtcgtg
     3781 atggcgagct cacgtcgcaa ccgggacacg acggccgcag ggagcactgc gacaccgatg
     3841 ccggcggcca cggcgcggca gatcgcgtcg ggatcggaca gcctggcgcg gtagcggggg
     3901 cgcagtccga ggggctgagc tcggccgccg aggtgttcct gaaaggggtt gccttcgacg
     3961 agaccgacga agggaaacgt gaggcactcg gcgaacgcga tccggtgccg ggcggtcagc
     4021 ggatgtcctg gcgtcgtcac cgcgacgacg ggatcgggcc gcaacggcag tgtgtgcagg
     4081 aggcccagct ccacggtgtc ggcgacgacg ccgagctctg cgcgaccctc ggcgatggcg
     4141 ccgacgatct gctggctcgg ccgttcctcc aggttgacgt cggtgtcggg gtgctcggcg
     4201 aggaagcgga tcagggcgtc gggcaggaag gtggacgcgg cagcggtgtt ggccagcagt
     4261 gtgaggtccg catgcatccc gtcggcgaac gcggcgaggt cgccacgcat ccgcgagagc
     4321 tggccgatca cggtgcgggc gtgtccgagc agctgctgcc ccgcggcgga cggatgcacg
     4381 ccccgtcggt gccgctgcag cagggacgtg cccagctccc tctccatcag gcgaatcctc
     4441 gcgctggccg aggggagcga gagatggcaa cgccgcgcgc catcagtgat gcttccactg
     4501 tccaccacct gcacgaacag ttggagatcc acgagatcga acgccatcac cgcaccgtac
     4561 cggcgccatg aggctctgtt cctcacagag gctgtggtct gatctttcgc attgtggcga
     4621 cctcgaggct tgcgcacggt ggtcctcatg gcatctgcgc gggatggctg gtcgttgatc
     4681 gggatctgcc tggggttctt cgtcgtcctg ctcgacgcga cgatcgtgaa cgtcgcactc
     4741 ggtgcgatcg aggcggcgct gggcggtggg ctcgggggtc agcagtgggt ggtcggcgca
     4801 tacaccgtcg cgttcggggc gctgctgctc agcgccggtg cggcgggcga ccggtgcggt
     4861 tcccgccgga tgtacctgct ggggctggcg ctgctcgccg gcgccggtat ggcctgcgca
     4921 ctggccggtg atctcccgca gctcctgatc gcgagggtgg tgcagggagc aggcgccgcc
     4981 ttggtgacgc cctgctcgct ggccctgatc acacaccggt tccccgacgc ccgcgctcgg
     5041 gcccgcgccc tcggcgcgtg gggcgggatc tccgggatgg ggctcgcggc gggcccggtc
     5101 ctcggcggcc tcctggtgga cgccgcaggc tggcgtgcgg tgttctgggc cgtggtgccg
     5161 ttcgcagtgc tcgccgccgc actcgtggtc gccaccacgc ccgagacgcc ccgccgcact
     5221 gcggtgcgtc ccgatctcgt ggggcaggcc ctggctgcgg tcgcactgac cgcgttgaca
     5281 gctgcgctgg tgaccgggcc gtcggccgga tggaacagct cgatggcgct catggccgct
     5341 ggagccgggc tggcgtcggc ggtcgccttc gcggcatccc agcgcctggt cagggcgccg
     5401 atgattccgc ccagtctgtt cgcggacagt cagttcggcg tggcggtcgg ggtgggtgcc
     5461 ttgttcaact tcggactcta cggaacgctg ttctgcgtcg cgctgtacct ggaacagacg
     5521 ctcgtaacct cgacgcggac gaccggcctg gtcctcctgc cgctgacggt ggtggtcgcg
     5581 atctgtgccg cgctgtcggg ccggatcaac gcccggacgg gcccgcgggt gccgatggtg
     5641 ctcgggctcg gcgccggtgt cctcgggtcc gtggcgctcg ccgggatggg cgcgggctcg
     5701 gcgcccgtgg tggtcggagc ggccgccgcg ctgctcggcg gagtgggcct ggcaatgccg
     5761 gcgatgacgt cggtcgcggt gagcgctgta ccggcggcga gtgccgggct cggtgccgga
     5821 gttctgaacg ctgcccggca ggctggaggg gccgtagggg tggcgggact cggggcggtc
     5881 ctgctcgacc cggccggccg gccgtccctg ctcgccgcga tgctggtggt cgccgcgggg
     5941 tacgtgctgg ctctgctgct caccgtcggg ctcatccgaa caccgtgatc gacgggaacc
     6001 tggccgaccg gctccagcgg ttggcgctca ctgtgccggt gaaagccggt cgcctctcac
     6061 tgctggatcg gggcgcccat cggcatggct tcgccgccga gttcctgcgt catcgcatcc
     6121 acgaagatgt aggcgtagat cgcgcagaac acgaacgtcg tgatgccgcc gagcgtggcg
     6181 aacagcgggt tgccgttcac cacgaaaccg agcacgagcg cgagcgtggt gcagacgacg
     6241 gcgaacgccg cggtgaacgc gagcggtagc cggagcgtgg ccacggtgag cgtcgcgaac
     6301 acgatgagcc aggacagcac gaaggtggac tggacctccc ggatctgctc cgctcgcaac
     6361 gacggctccg tggcgatacc gaaccagccg ttgatgacgc ccagcagcag gaacccgaag
     6421 ctcagccaga acgcggcgaa ggtgccgaag atcgcggccg ccgcgccctg ccccacccgc
     6481 gccgcccaga cgcacgccac cagcaggaac aggcccgacg atgacgtgat cgcggggatc
     6541 atgaccccgg ggagtgtggg cgtgtcgagg tagccgatga gccacaggcc cagcgtgagg
     6601 ctgccgggca gcagagccgg cagaccgagc agagcggggt ttccgccggc ggtggccgac
     6661 gcctcccgag tcacgtttcc cgtgcgcggc cgggcgtcgc tcacgctcat ccactcgctc
     6721 ctcgttgaga cggccgcctc accggcatcg atacgccgga tggaccacat tgtgggcccg
     6781 atcgcctgat cgcgacaccg tcgccggccc gaccggggtg caggtgcggg aggagaccgg
     6841 gtcttgcacg acacgggcct gttcggacgc ggtgcagcgg tggatcggca ccacaggaaa
     6901 gatcgacagt tgtcggctcg gccgagccga atgtgcccta tcccggttgc gggggcccgc
     6961 tggacagccg gagcggattg gctccgttca ctcgggcgtg acctcggcca tctcagtcca
     7021 ggcttcgccg tccagttcct ggtagttgat ccgtggccgc tccgcaacca tcgccggcat
     7081 ccaggcgaag aacttcgtgg cgtggtcggt ggcgacgtgc gctgcgccgg cttccgagtc
     7141 cgcaaacacc tcgatcatcg cgaactcgtt cgggtcgtcg ccgttctgga agtagtcgaa
     7201 cgagaggttc ccgggctcgc ttcgcacgtc ggccgtgtac tgcctgattc cggccagcca
     7261 ctcgtcgtgc ttctccggtc ggatcttcgc cctcaggacg atgagaatca tgcggggcct
     7321 ccatgggctg gcgttcactc ggctgccggc aacattgccg acctggtaaa aggcagtctg
     7381 ccacgggtgg gcacgaccca tcagcgatcg ctgaggcagg ctcgttcacg tcaccggcaa
     7441 ccgagggacc ggcgttcggg ccagccacgg cgacggcctg gggatggcag ggtgaccgcg
     7501 tgcacgacga caccggatac gacggcgccg gatacgacga cagcgactac atcgatctgg
     7561 acctgtccga ggtgcaggtg ggggacgagc cgtggcggca gcggcggttc gtgggctgcc
     7621 ggttcgtcga cgccgacctg cgtggcctgg tcaccgagtc ctgcacgttc gacgagtgcg
     7681 acttcaccgg cgccgacctc ggtgactcca gccaccgcgg cagcgcgttc cgcacctgca
     7741 tgatccggcg ggccacgctg ggcggtgcca ccttccgcgg ctgctcactg ctcggcacct
     7801 ccctcatcga ctgccggctg cggccgcttg tcgtcaccga ctgcgacctc acacttgccg
     7861 ccctcggccg ggccgacctg cgtaagacgg acctgtccgg gctgcggctg cgcgaggcga
     7921 acctcgtcga ggccgacctg cgcgaggccg acctgcgggg cgcggacctc agcggcgccc
     7981 ggctgctgca cacccggctc gagggcgccg acctgcgggg cgcccgcatc aacccggacg
     8041 gactcgtgca ggcccggctc accggggcca ccgtcgacgc cgagcaggcc ctcgccttcg
     8101 ccgttgccca cggcctccgc atcgcctgat cccgcgcccc gggtcgcgag atgagatggg
     8161 gcacccggcc atcactcagg gccgacgcgc gggctgggag aatggggccg tggcgaccct
     8221 gatggacggc aaggcgactc tgaacgagat cctgggcgag ctcgcgagcc gggtcgagaa
     8281 gctgcaggcc aacggcgtga cgccgggcct gggcaccctt ctcgtcggtg atgaccccgg
     8341 atcgcattcc tacgtccggg gcaagcaccg cgactgcgcg aaggtcggga tcacctcgat
     8401 ccagaaggag ctgccggccg acgcgacgca ggccgacgtc gagcgggcga tcgacgagct
     8461 caacgcggac cccgcgtgca ccggcttcat cgtgcagctc ccgctcccgc agggcctcga
     8521 cgccggcgcc gccctggagc gcatcgatcc cgcgaaggat gccgacggac tggcacccgt
     8581 cagtctcggt cgcctcgtgc tgggcgagcc cgggccgctg ccgtgcacgc cgcgcggcat
     8641 cgtcgagctc tgccgccgct acggcgtgga gctggccggc gcgcgcgtga ccgtggtcgg
     8701 ccggggcgtg acggtgggtc gtccgctcgg gctgctgctc acgcggcgca gcgagaacgc
     8761 cacggtgacg ctctgccaca ccggaacgcg cgacctcgcc gccgaggtgc gccgcgccga
     8821 tgtggttgtg gccgcggccg gtgtacccgg cctgatcacg gccgacatgg tccggccggg
     8881 cgcgacggtg ctcgacgtgg gcgtcacccg caccgatctc ggcctcgtcg gtgacgtcgc
     8941 gcccgaggtg gtcgaggtgg ccggccgact cgcgccgatg ccgggtggcg ttggcccgat
     9001 gacgcgagcg atgctgctga cgaacgtggt ggaggcagcc gaacggagct tgtcggagtg
     9061 agtatcgacg ccgaacggag cttgtcggag tgaagcatgg acgccgaacg cgcgtccggg
     9121 gtgtgatggc gggcgacctt cgcacacggc tccccgtgca tgcgccgatg atcctggtga
     9181 tggtcatcgc ggcagcgggc atggtccgcg tgctcaccca acactggcgc gagggtgcgg
     9241 tgctgctcgg cggggccctg atcgcggctg cggtgctgcg cgtcctgctc ccggtggacc
     9301 gcgtcggcct gctggcgatc cgtagccgcg cgaccgacgt cctctgctat accgcgttcg
     9361 gggtggtgat gacggcgctg gccgtgacga tcacacgcgg ctcgttcgac ttcccgtgaa
     9421 cgctgccgcg cgcgctcaga ccgcgcccac tgcacaggac ggcgggcgag cagtaccccg
     9481 agggtggcgt agccggccag cgcaggtgca ggccgcccac aagccgccgg ccgtcgcgcc
     9541 gaggacgacc gtgacgggca ggaccacgcg accgggcgat cgtgcgcgcc agggaagtgg
     9601 gaatgaatgg gacgcgccgg gcctccggtg ccgccgcgga cggcgcgtcg gtgcacgatc
     9661 ggtcgcatga ccgatgacgc ccgcgcggcc cgcatcgtcg acaccctcgt cgaggcgttc
     9721 tccgacctga tggcggcaga cccggctgcg tttcggacga agttccgcaa gatggcagcc
     9781 gacccgttcg cgttctaccg cggcagcgcc tgcgtgttct acgcggacat ggcggagcgg
     9841 gacgacccgt gggccgacga gcggacgagc cgggtctgga tccagggcga tctgcacgcg
     9901 gagaacttcg gcacctacat ggacggcgac ggggtgctga tcttcgacgt caacgacttc
     9961 gacgaggcat atgtcgggca cttcacctgg gatgtcaccc ggttcgctgc gagcctggcg
    10021 ctgctgggct ggcgcaaggc gatccccgac cccgacatca cgacgctcgt agagcggtac
    10081 acccgggcct acctggacca ggtgcgctgg ttcgtcgacc aggccgacga ccgcagcgtc
    10141 tcgctgcggc tggccaccac cgacggcgtg gtccagcagg tgctccagct ggcccggctg
    10201 cggacgcggg tagagctgct ggacaagatc accgagcccg aggggttcga tcgcattctg
    10261 cgtgacgggc ccggcctgcg ccgcctcgac gacggggagc gcgcgatggt cacggacgcg
    10321 ttcgagcgct acctcgactc catcccggag tcgaagaagt tccgcggggt cacctacacg
    10381 gtgaaggacg tggccgggcg gtccggcttc ggcatcggca gcgcggggct cccggcgtac
    10441 acggtgctga tcgagggcta caaccaggcg ctggacaacg acgtggtgct ctcgatgaag
    10501 cagggcaacg tcgcggcgcc ctcgcgggtg gtgaccgacc ccgctatcgc cgctgggttc
    10561 cgccaccacg gccaccgcac ggtggtgtcg cagcgcgcgc tgcaggccca cgccgacccg
    10621 ctgctgggct ggaccgacct cgacggcgtc ggattcgtcg tcagcgaggt gtcgccgtac
    10681 gaggccgacc tggactggtc ggagctcacc gagccgtcgg agatcgcgcc ggtggtcgac
    10741 tacctgggcc gggcgacggc gaaggtgcac tgcgtggccg actccgacgc cgaccactcg
    10801 gtcgtcccat tccagacgga ggacgcgatc atgggagtgg tcgccgggcg ggaggacgag
    10861 ttcgtggcct gggtggtgga gttcgcccac gagtacgcgg cccaggtgcg ggccgaccac
    10921 gcgctgttcg tcgaggcgtt ccggagcggc gcgatccccg gagtcgccag cacctcgtga
    10981 gtggatacga gtgctctggc acctgtatcc gcgcacgggc gcgtcagcgc aggtagatcg
    11041 tgcgcacgag gtcctcgatg gacgactcga agacggagac gtcgcgcagc tccacgtgct
    11101 gggcaacggc cgcgatcagc tcggcggcgg tggtcgcgcc cggcgagaac tcgaggcgtt
    11161 gccgcaaccc ctgcgcctcg acggtgacgt cgacgacccc ggtgaggccg agaagcggcg
    11221 gtgcaggcgc catgagctcg acgacgagcg cccggtggcc ggggaaccgc tgccgcagca
    11281 cggccagctc gtcgtccacc agcacgcggc cctgatcgat gaccaccacc cgccggcaca
    11341 gccgctcgac gtcgggcagg tcgtgcgtcg tgagcagcag cgtgacgtcg ccgcgcgcgt
    11401 tcatcgcgga caggaacgag cgcagccgct ccttgctcgt gaggtccagg ccgatcgtcg
    11461 gctcgtccag cacgagcagc tcgggggagt gcagcagcgc ggccgtgacc tcgccgcgca
    11521 tccgctggcc cagcgacagc tgccgcaccg gcgtacgcag gaacccgccc atgtcgagca
    11581 gctccacgca ctcgtccagc cgctcgcggt agcgcgccgc cggtacgcgg tggatggcgc
    11641 cgtgcaggtc gaagctttcg accagcggca gatcccacca cagctggctg cgctgcccga
    11701 acaccacccc gatgcggcgg gcgagctccc ggcgctcgcg cacggggtcg agcccgcaca
    11761 cgctcgcccg tcccgaggtc gggacgagca ccccggtcag catcttgatc gtcgtggatt
    11821 tgccggcgcc gttcgggccg aggaacccga ccgcctcccc ccgagccacg gtgagcgtca
    11881 cgtcctccac ggcggggacg cggcgccgcc gcagccgcgt gccgacgagg aactcacggc
    11941 gcagcgcctc ggtctggatg atcatgaccc cgtcccctgg tagtgccgga cgccggtgcg
    12001 ccagaccagc ccggctcccg tcaccgcgat cgccgcgacg agcggagagc tccactgcag
    12061 cgcctccggc agtccgagcg ggtcgggccg ccccagcagc gcgagcgccg ggaagtacgc
    12121 cacgaacgcc cccggcacga cgtagcacat
//
